LOCUS       DAYASV010000001       105555 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYASV010000001 DAYASV010000000
VERSION     DAYASV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 105555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.001"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(100..1368)
                     /locus_tag="ACGI68_000001"
     CDS             complement(100..1368)
                     /locus_tag="ACGI68_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136465.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIIDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            complement(1379..2191)
                     /locus_tag="ACGI68_000002"
     CDS             complement(1379..2191)
                     /locus_tag="ACGI68_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136466.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTESWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            complement(2200..3399)
                     /locus_tag="ACGI68_000003"
     CDS             complement(2200..3399)
                     /locus_tag="ACGI68_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136467.1"
                     /translation="MKLKSKISIFIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEGSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILASKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            complement(3411..4235)
                     /locus_tag="ACGI68_000004"
     CDS             complement(3411..4235)
                     /locus_tag="ACGI68_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0136468.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINIITLFRKYYNLIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKVSL"
     gene            complement(4228..4944)
                     /locus_tag="ACGI68_000005"
     CDS             complement(4228..4944)
                     /locus_tag="ACGI68_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0136469.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            complement(5016..5372)
                     /locus_tag="ACGI68_000006"
     CDS             complement(5016..5372)
                     /locus_tag="ACGI68_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0136470.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            5728..6186
                     /locus_tag="ACGI68_000007"
     CDS             5728..6186
                     /locus_tag="ACGI68_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0136471.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            6190..7152
                     /locus_tag="ACGI68_000008"
     CDS             6190..7152
                     /locus_tag="ACGI68_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0136472.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            7146..7895
                     /locus_tag="ACGI68_000009"
     CDS             7146..7895
                     /locus_tag="ACGI68_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0136473.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDIMPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(7961..8263)
                     /locus_tag="ACGI68_000010"
     CDS             complement(7961..8263)
                     /locus_tag="ACGI68_000010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0136474.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(8402..9034)
                     /gene="thiE"
                     /locus_tag="ACGI68_000011"
     CDS             complement(8402..9034)
                     /gene="thiE"
                     /locus_tag="ACGI68_000011"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFH0136475.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            complement(9057..9845)
                     /gene="thiM"
                     /locus_tag="ACGI68_000012"
     CDS             complement(9057..9845)
                     /gene="thiM"
                     /locus_tag="ACGI68_000012"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFH0136476.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDALSKNSNIEFLN"
     gene            complement(9869..10663)
                     /gene="thiD"
                     /locus_tag="ACGI68_000013"
     CDS             complement(9869..10663)
                     /gene="thiD"
                     /locus_tag="ACGI68_000013"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFH0136477.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     regulatory      complement(10763..10855)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(10948..11259)
                     /locus_tag="ACGI68_000014"
     CDS             complement(10948..11259)
                     /locus_tag="ACGI68_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0136478.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     gene            11532..12776
                     /locus_tag="ACGI68_000015"
     CDS             11532..12776
                     /locus_tag="ACGI68_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136479.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYDMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDGLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            12973..13842
                     /locus_tag="ACGI68_000016"
     CDS             12973..13842
                     /locus_tag="ACGI68_000016"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HFH0136480.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(13896..14084)
                     /locus_tag="ACGI68_000017"
     CDS             complement(13896..14084)
                     /locus_tag="ACGI68_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFH0136481.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(14244..14834)
                     /gene="epsC"
                     /locus_tag="ACGI68_000018"
     CDS             complement(14244..14834)
                     /gene="epsC"
                     /locus_tag="ACGI68_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFH0136482.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            complement(14861..15769)
                     /gene="cysK"
                     /locus_tag="ACGI68_000019"
     CDS             complement(14861..15769)
                     /gene="cysK"
                     /locus_tag="ACGI68_000019"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFH0136483.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            complement(16006..16632)
                     /locus_tag="ACGI68_000020"
     CDS             complement(16006..16632)
                     /locus_tag="ACGI68_000020"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFH0136484.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEKFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVDDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            complement(16651..18708)
                     /gene="kdpB"
                     /locus_tag="ACGI68_000021"
     CDS             complement(16651..18708)
                     /gene="kdpB"
                     /locus_tag="ACGI68_000021"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFH0136485.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            complement(18733..20409)
                     /gene="kdpA"
                     /locus_tag="ACGI68_000022"
     CDS             complement(18733..20409)
                     /gene="kdpA"
                     /locus_tag="ACGI68_000022"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFH0136486.1"
                     /translation="MVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDNFI
                     YKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGLSF
                     NTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKKKT
                     LGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGPVA
                     ALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKNKK
                     QGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSSLF
                     TTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVFLC
                     GLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGFHG
                     LSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGVVMFFGRYVSIIVLLAISSLLASK
                     KAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            complement(20456..20548)
                     /gene="kdpF"
                     /locus_tag="ACGI68_000023"
     CDS             complement(20456..20548)
                     /gene="kdpF"
                     /locus_tag="ACGI68_000023"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFH0136487.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            complement(20959..21591)
                     /locus_tag="ACGI68_000024"
     CDS             complement(20959..21591)
                     /locus_tag="ACGI68_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0136488.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            complement(21963..22925)
                     /locus_tag="ACGI68_000025"
     CDS             complement(21963..22925)
                     /locus_tag="ACGI68_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0136489.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            complement(22946..23896)
                     /locus_tag="ACGI68_000026"
     CDS             complement(22946..23896)
                     /locus_tag="ACGI68_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0136490.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMFLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            complement(23919..25415)
                     /locus_tag="ACGI68_000027"
     CDS             complement(23919..25415)
                     /locus_tag="ACGI68_000027"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0136491.1"
                     /translation="MSKILEIQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEEYMKICEDFDFNIDPHIVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVSD
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            complement(25428..26612)
                     /locus_tag="ACGI68_000028"
     CDS             complement(25428..26612)
                     /locus_tag="ACGI68_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136492.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYKKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISSYFKSKGLKIGIDAFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            complement(26605..27555)
                     /locus_tag="ACGI68_000029"
     CDS             complement(26605..27555)
                     /locus_tag="ACGI68_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFH0136493.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINDNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKMDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            complement(27557..28651)
                     /locus_tag="ACGI68_000030"
     CDS             complement(27557..28651)
                     /locus_tag="ACGI68_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0136494.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISSITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVDS
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            29126..29395
                     /locus_tag="ACGI68_000031"
     CDS             29126..29395
                     /locus_tag="ACGI68_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFH0136495.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            29513..30265
                     /locus_tag="ACGI68_000032"
     CDS             29513..30265
                     /locus_tag="ACGI68_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0136496.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYAE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIYIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(30468..31733)
                     /gene="hisD"
                     /locus_tag="ACGI68_000033"
     CDS             complement(30468..31733)
                     /gene="hisD"
                     /locus_tag="ACGI68_000033"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFH0136497.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(31997..32479)
                     /gene="cdeM"
                     /locus_tag="ACGI68_000034"
     CDS             complement(31997..32479)
                     /gene="cdeM"
                     /locus_tag="ACGI68_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HFH0136498.1"
                     /translation="MENKKCYSEDWYERGESTAKWFQNDREEYEREAYDEDRERRGSN
                     CGCSDSGENRPRNCERFRREAEIREREAREAFCESSEKKKEALAYECEARKLWEEAEK
                     YWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCNCCRK
                     "
     gene            complement(32596..33813)
                     /locus_tag="ACGI68_000035"
     CDS             complement(32596..33813)
                     /locus_tag="ACGI68_000035"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFH0136499.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     misc_binding    complement(33898..34133)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(34199..34439)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(34522..36378)
                     /locus_tag="ACGI68_000036"
     CDS             complement(34522..36378)
                     /locus_tag="ACGI68_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0136500.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGTYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     gene            complement(36895..37734)
                     /locus_tag="ACGI68_000037"
     CDS             complement(36895..37734)
                     /locus_tag="ACGI68_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFH0136501.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            complement(37948..39645)
                     /locus_tag="ACGI68_000038"
     CDS             complement(37948..39645)
                     /locus_tag="ACGI68_000038"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFH0136502.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            complement(39711..40301)
                     /locus_tag="ACGI68_000039"
     CDS             complement(39711..40301)
                     /locus_tag="ACGI68_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFH0136503.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            complement(40411..41208)
                     /locus_tag="ACGI68_000040"
     CDS             complement(40411..41208)
                     /locus_tag="ACGI68_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFH0136504.1"
                     /translation="MEKREKRIIISSLLSVSILMGLASIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            41564..42085
                     /locus_tag="ACGI68_000041"
     CDS             41564..42085
                     /locus_tag="ACGI68_000041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0136505.1"
                     /translation="MKFERLRTSENKLYYKAMELYKISFPFHKQRKSSLQAEILKNKE
                     YQFNLIYDKNQFVGIILCWETDNFIYVEHFCIFPEIRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHKKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            complement(42505..43719)
                     /locus_tag="ACGI68_000042"
     CDS             complement(42505..43719)
                     /locus_tag="ACGI68_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0136506.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(44184..45119)
                     /locus_tag="ACGI68_000043"
     CDS             complement(44184..45119)
                     /locus_tag="ACGI68_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HFH0136507.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            45407..45973
                     /locus_tag="ACGI68_000044"
     CDS             45407..45973
                     /locus_tag="ACGI68_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0136508.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRKRVNKND"
     gene            complement(46164..48200)
                     /locus_tag="ACGI68_000045"
     CDS             complement(46164..48200)
                     /locus_tag="ACGI68_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFH0136509.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPAGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYRLKDDKISYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(48369..49046)
                     /locus_tag="ACGI68_000046"
     CDS             complement(48369..49046)
                     /locus_tag="ACGI68_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0136510.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            49389..49517
                     /locus_tag="ACGI68_000047"
     CDS             49389..49517
                     /locus_tag="ACGI68_000047"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136511.1"
                     /translation="MSRIERRKKDRESKLAKVKTALSILPILINLIDKLIKLLKDL"
     gene            49763..49939
                     /locus_tag="ACGI68_000048"
     CDS             49763..49939
                     /locus_tag="ACGI68_000048"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136512.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            50100..50228
                     /locus_tag="ACGI68_000049"
     CDS             50100..50228
                     /locus_tag="ACGI68_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136513.1"
                     /translation="MGRLERRKNKRENKFNKIKNAFSFTLVLINLVLAILRLIKEL"
     gene            50457..50642
                     /locus_tag="ACGI68_000050"
     CDS             50457..50642
                     /locus_tag="ACGI68_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0136514.1"
                     /translation="MILLEQRKLRIGFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(50684..53032)
                     /locus_tag="ACGI68_000051"
     CDS             complement(50684..53032)
                     /locus_tag="ACGI68_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0136515.1"
                     /translation="MKNLSKKAVILSLSLVVVSPLVHKINAQDIEKNREVRYETRKAN
                     NLENMTMEEAFPDENFRKVICDKLSILDDGNPIGISQKAIIESTKGLTLGSKSIKNLS
                     GINYFIGLENFSCRGNNLTSLDLSSNINLKELYCNENQLTSLDLSNNRELTTLHCGEN
                     NLTDLVIENSKLDELNCRKNNLKSLDITKATNLTTLLCFENELTSLNLSKNQKLKILK
                     CDYNNLNDLDISTNLELQDLNCSFNQLENINLDNNKELVKLICHNNKLQNLNLKNHEK
                     LVKLYCNQEQLSTLNVENCINLEDLTCSRSNLKNLDISSNKNLKYLECVANKLTNLNI
                     ENNIELLELYCYDNEIKALDISKNIDLENLTCYKNKLDSLNTSKNKNLKYLYCSQNEL
                     TSLDVTKNTELVRLYCGENRLTSLDVSKNTKLKELDWSNQKKSTSTGGSSGSGGGSSS
                     TNEESSEPTSTPSKEKLTGVDRNETSVKISQKGWNKADNIVLINDSSISDALSATPFA
                     KSKDAPILLTKNNNLNKLTEKEINRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRI
                     SGNDRYETSIKLAKELDKNSNLSKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQND
                     DMKDIKDLIANKNISKSYVIGGESLFNNKEVNNTLPSVTKIAGSDNNKEVNNTLPSVT
                     KIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLVDALSVGVLAGKTESPVV
                     LVGNGLDNSQKELIKNKKFKNITQIGGNGNEKAFNEVENLVK"
     gene            complement(53189..54001)
                     /locus_tag="ACGI68_000052"
     CDS             complement(53189..54001)
                     /locus_tag="ACGI68_000052"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0136516.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKTYKIPRVNSGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICNKLGTVFRNRRAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKL
                     GHEGIAKLIVEGVLNKNINNEGVKQMYKHTIVYDGEVDKISATVVGWGYNDGKILICD
                     IKDYVPGQTQNLYVIGGAACEKIGSMTKEKFTMIKGNDRFDTLYKALDFINR"
     gene            complement(54080..54943)
                     /locus_tag="ACGI68_000053"
     CDS             complement(54080..54943)
                     /locus_tag="ACGI68_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFH0136517.1"
                     /translation="MVEVKEEKTFDEQIDILKSRGLIINDKEDAKFVLSNVNYYRFTA
                     YLLSFKNDDGSYKEGTTFEEVYDIYRFNKEFRILLTDLLGSIEIAFRTYIAYTLAIKH
                     GACGYLERESFKDEKFYINFLTALEREKSNNSDKLFIIHHKEKYEGKLPIWVATEIMT
                     FGMLSKLYSNMLPEDTRYIKNNLCRVNTLLVKSWLQSLTQVRNQCAHYGRIYNNNFRI
                     ITIKNEYKKYNLDNKKIFSYILAMKHLTMDKLIWNSFFIKLQKLINDYNNSIDLKLIG
                     FPNNWIEILAK"
     gene            complement(55204..55461)
                     /locus_tag="ACGI68_000054"
     CDS             complement(55204..55461)
                     /locus_tag="ACGI68_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0136518.1"
                     /translation="MDNLISFIPEQLLILVAALYVLGVGFKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGINQTYKQLKDGEK"
     gene            complement(55465..55755)
                     /locus_tag="ACGI68_000055"
     CDS             complement(55465..55755)
                     /locus_tag="ACGI68_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136519.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            complement(55786..56352)
                     /locus_tag="ACGI68_000056"
     CDS             complement(55786..56352)
                     /locus_tag="ACGI68_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136520.1"
                     /translation="MTFKELVNKVRNLVLEAKKVTIEDTENKFTSENVEEALKECINR
                     ADEAFQEADSGKELIATAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            complement(56386..56568)
                     /locus_tag="ACGI68_000057"
     CDS             complement(56386..56568)
                     /locus_tag="ACGI68_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136521.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAIED
                     YIIKNSGVVEGAEPTI"
     gene            complement(56568..56873)
                     /locus_tag="ACGI68_000058"
     CDS             complement(56568..56873)
                     /locus_tag="ACGI68_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136522.1"
                     /translation="MTEYVEVGRRIFFDEEGKIIFYEGQSKGNVPDRKNIKKIEYIDL
                     EYDYVDYDKYKIIGIDIRTKQPILEEIPVYMSEEGKRIQELENQILIAENEKSGGLL"
     gene            complement(56897..57502)
                     /locus_tag="ACGI68_000059"
     CDS             complement(56897..57502)
                     /locus_tag="ACGI68_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136523.1"
                     /translation="MIEKLNENASLSDLITTFENVKDELQIGKNNIASTLGNPFVGTD
                     KLSVTKTKIQTLKNTLAISLSMKNISAQSTETLHSLIGKVAGIANIFIAQGECTQVGE
                     KTFQLYQVDNNTPKTCSANAISFNLAFTPKFIIVYRNAYNIGLCSFVFYSSFRNIITA
                     VPQPSNFGEMYMPNTFDCVVGHTCILPVKDYKIPHFFYAIA"
     gene            complement(57713..57847)
                     /locus_tag="ACGI68_000060"
     CDS             complement(57713..57847)
                     /locus_tag="ACGI68_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFH0136524.1"
                     /translation="MWYKIIKKYYEDELWTKEQVKTAVEKNKITTIEYKNIVGEDYIV
                     "
     gene            complement(57848..58213)
                     /locus_tag="ACGI68_000061"
     CDS             complement(57848..58213)
                     /locus_tag="ACGI68_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136525.1"
                     /translation="MIKIWDKKEKINGVSAEEILKGNYDFQTSEVFLILDDYNRVTNI
                     ESVNTIKSIYKLDKNLTALETAEKYLEMHKKQEEDIKAHEELEKNSKNATIATHEEVK
                     ALREETATLTFAMMEKELI"
     gene            complement(58231..59364)
                     /locus_tag="ACGI68_000062"
     CDS             complement(58231..59364)
                     /locus_tag="ACGI68_000062"
                     /inference="COORDINATES: protein motif:HMM:NF025330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136526.1"
                     /translation="MTIKLEEDSSIKEVMDSFENIKNDLQNAKDNLASTLGSPFVSTD
                     SFSTTKTKIQTFKSTLATNLTKKGVSTSSNETVQNMVSKVDDIELGNTDIPNWYKPKN
                     IIFDGVTSALQQYPPCVAVENYIYFLCNNSLGLSLLRYDTLTNVNSVLAKPSVCDDAL
                     MVYYDGYIYRIGGYSNSSANSTCQKYNIKTNTWSYISNMITPRGGTCGNTYSNEIHVL
                     YGYTDTNSNSTASAISEYYKISTNTWSTKTSFPPSRDRRLFVCAKTGTYYFCITGATD
                     PNNIWETYINRYDAGNGALTKIEGWYKSFATSINDMLYTVYYGDTYLYVKGYNQNGRV
                     FSKEYVKKNSTFPYNPLLKAVSVKNKYIYYYENNMYKCFIPEL"
     gene            complement(59557..60702)
                     /locus_tag="ACGI68_000063"
     CDS             complement(59557..60702)
                     /locus_tag="ACGI68_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HFH0136527.1"
                     /translation="MATKLEENAKLREVIDTLETTKDDLQTVKNNIADVLGLPFTGND
                     KLETTKNTLSSIRSTFVSNLSKKGVSTVSNAPFKTLAENVGKIEQGNMIVPPWYVPKN
                     IFIKALSTAVKDKTPCVSAEKKIYFINGYTGTSGLNNLCYDTVLNTTTTKNIPSSFVE
                     SALFFYDGYIYKIGGFSGYSAPTAICQKYNILTSSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSTYKIVVFGL
                     KSSISSHYFSTINYDPVSGTYSYAGEGFDSNTFAVQLGSTLYVSDYGYKAGSGKCGVV
                     IFSSSGIKKLTFDKGAVPNYSYFGASVDGRQIYFILNSEITCFIPEL"
     gene            complement(60719..61795)
                     /locus_tag="ACGI68_000064"
     CDS             complement(60719..61795)
                     /locus_tag="ACGI68_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0136528.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTSNIETAKTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESG
                     FKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEIRSEARSELNVYVINGNA
                     ETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISITDANDRFTSDKLDGVLE
                     EIMVEISGQRTKGITIVNNLIDMI"
     gene            complement(61807..62424)
                     /locus_tag="ACGI68_000065"
     CDS             complement(61807..62424)
                     /locus_tag="ACGI68_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFH0136529.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            complement(62417..63466)
                     /locus_tag="ACGI68_000066"
     CDS             complement(62417..63466)
                     /locus_tag="ACGI68_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0136530.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEKTYKMKCETTGPVFELGQLIPIEYVDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSSDLVNLVQEEIDPLQNQGEGLGLAPIGHRVTVEGVTSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TIDSNSIVVRGEVVG"
     gene            complement(63467..63886)
                     /locus_tag="ACGI68_000067"
     CDS             complement(63467..63886)
                     /locus_tag="ACGI68_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0136531.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLNIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            complement(63891..64151)
                     /locus_tag="ACGI68_000068"
     CDS             complement(63891..64151)
                     /locus_tag="ACGI68_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFH0136532.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESINPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            complement(64165..66057)
                     /locus_tag="ACGI68_000069"
     CDS             complement(64165..66057)
                     /locus_tag="ACGI68_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0136533.1"
                     /translation="MADDLVLANDRDVRLIIAHWEDFYEPAVIDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKLSRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLDLGDIELQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDILLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            complement(66050..66751)
                     /locus_tag="ACGI68_000070"
     CDS             complement(66050..66751)
                     /locus_tag="ACGI68_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFH0136534.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFCRGQFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNMKVSLEEYNIVEDAENGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWLICKKQLGNGSLYKKVYELNKSMMDKANKGKN
                     LSKYTIYKGQVLKLG"
     gene            complement(66767..69160)
                     /locus_tag="ACGI68_000071"
     CDS             complement(66767..69160)
                     /locus_tag="ACGI68_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFH0136535.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMPITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVQGITTGTVLIIQGLGWITNAALNVANVFAQNWSIIAPAVYG
                     VVAAIAIYKGILLASAIVTGVSSFVNALYALGAYKACAALAAQELALFGKISAQTIEA
                     MVTAQATAAQYGLNAALYACPLVWIVLGFIAVIAVIFMVVAAINKFAGTSLTVLGVIV
                     GAVFAAVATIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DSVNNAGNALGKAFVDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNRWVGDAPEKVKLERMGYKDIGAEFQKGNALGTKWQNAISNKLKDTFDINK
                     MLEDAKDKLGLKDLWDKDNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNINSEADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            complement(69225..69740)
                     /locus_tag="ACGI68_000072"
     CDS             complement(69225..69740)
                     /locus_tag="ACGI68_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0136536.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREEE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     repeat_region   69871..70758
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=69936..69964
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(70834..70983)
                     /locus_tag="ACGI68_000073"
     CDS             complement(70834..70983)
                     /locus_tag="ACGI68_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136537.1"
                     /translation="MHENLLDIDRIELIKELGNIFEKMKNENPDEFYRFVSLVKEECR
                     KKREK"
     gene            complement(71035..71850)
                     /locus_tag="ACGI68_000074"
     CDS             complement(71035..71850)
                     /locus_tag="ACGI68_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFH0136538.1"
                     /translation="MMNELMNFEGKDIEVFEFEGQILFNPKHVAECLDISDVNSSIRK
                     FNDNQVVKLTNSKMHNMQFRKLHNTGENFLTESGVYKLIFKSKKEEAERFQDWISDEV
                     LPAIRQTGAYITNNADPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSKLLTA
                     KTLYKKAGIDLPIEINEEEHYFDTKQIASKLKLYSKSNKPAQMAVCEIIKKIDLEDSE
                     VKGVWETNGSWTGTVNKYTESVIDKIRNWIEENNRPTKIAGEKKNYHVVYKIE"
     gene            complement(71968..72150)
                     /locus_tag="ACGI68_000075"
     CDS             complement(71968..72150)
                     /locus_tag="ACGI68_000075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136539.1"
                     /translation="MSNKLGRPPKENSKKLRFEVRLNQEQADILNECAENLKISKTDV
                     VIRGIELVKEKIDKNK"
     gene            complement(72337..72861)
                     /locus_tag="ACGI68_000076"
     CDS             complement(72337..72861)
                     /locus_tag="ACGI68_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136540.1"
                     /translation="MWGKFKKLSLLKKILVIFLIYFVVFTVSMMIHQAIRDSKNRDEV
                     NEGNITKENIISEREKEDIYKQEMQAKVDSMIPEDLKDKTTYYVNILNPTKGEGYIVS
                     IQVENSRFNDENECRNFTKEFVNNIKDMNDIHSVRISFIVDVTLTYNVFLDDWNNIKN
                     NVNLIDDLDFSSGN"
     gene            complement(74090..74251)
                     /locus_tag="ACGI68_000077"
     CDS             complement(74090..74251)
                     /locus_tag="ACGI68_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136541.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(74592..75032)
                     /locus_tag="ACGI68_000078"
     CDS             complement(74592..75032)
                     /locus_tag="ACGI68_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0136542.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(75104..75574)
                     /locus_tag="ACGI68_000079"
     CDS             complement(75104..75574)
                     /locus_tag="ACGI68_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0136543.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(75591..76901)
                     /locus_tag="ACGI68_000080"
     CDS             complement(75591..76901)
                     /locus_tag="ACGI68_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HFH0136544.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFVSATRATSSLSDRGIVAIPLEL
                     DWGIDKDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNTKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDKDLVESSLIYWAAGAIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVS"
     gene            complement(76902..77087)
                     /locus_tag="ACGI68_000081"
     CDS             complement(76902..77087)
                     /locus_tag="ACGI68_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136545.1"
                     /translation="MIILSKTLSKEDNYKFTKEQIVNSKKYVNRKDLLNAILKENDLY
                     SFSEVEDRINKFMKGVS"
     gene            complement(77071..77508)
                     /locus_tag="ACGI68_000082"
     CDS             complement(77071..77508)
                     /locus_tag="ACGI68_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0136546.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYLSGRTLKKNSFDVHYFPKSNDKSFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTDKMDTVELFGGLKRGDNFE"
     gene            complement(77501..77926)
                     /locus_tag="ACGI68_000083"
     CDS             complement(77501..77926)
                     /locus_tag="ACGI68_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFH0136547.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTTYASYVEYGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            complement(77926..78273)
                     /locus_tag="ACGI68_000084"
     CDS             complement(77926..78273)
                     /locus_tag="ACGI68_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136548.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSCKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(78267..78668)
                     /locus_tag="ACGI68_000085"
     CDS             complement(78267..78668)
                     /locus_tag="ACGI68_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136549.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLVAYLTTYGKNKILTFRCLRW"
     gene            complement(78678..78911)
                     /locus_tag="ACGI68_000086"
     CDS             complement(78678..78911)
                     /locus_tag="ACGI68_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136550.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(78911..79810)
                     /locus_tag="ACGI68_000087"
     CDS             complement(78911..79810)
                     /locus_tag="ACGI68_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HFH0136551.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKYFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            complement(79822..80385)
                     /locus_tag="ACGI68_000088"
     CDS             complement(79822..80385)
                     /locus_tag="ACGI68_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HFH0136552.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANEAIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(80455..80703)
                     /locus_tag="ACGI68_000089"
     CDS             complement(80455..80703)
                     /locus_tag="ACGI68_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136553.1"
                     /translation="MDNKFKNLQHLIDSIDLGLDIEFDLYNKPYNISIGDDDTRFITL
                     CPNGDTKYYKNGKDMVDNYNIDGKLLKDLWKDIQIVNM"
     gene            complement(80705..82240)
                     /locus_tag="ACGI68_000090"
     CDS             complement(80705..82240)
                     /locus_tag="ACGI68_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFH0136554.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINNIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWATDGLNFSERIWGKHRHALVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLRTSNICRE
                     LDGKVFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSNMKYN
                     EWYEKHIEGRLGKEKADTLRKMQLNESKDRKQFEEYKKVLGNEIPSKFDEYQHMKYND
                     TIRYEEAKKLYKDVNWQVKNQRNLTSGSVHSVPFESKPNSVFDNYKDGKLIQRRYYGN
                     TGKPRLDLDLTDHRNPKQHKIVPHKHDWLADENNHDKVKREKDMELTKAHKIANKDIL
                     KGE"
     gene            complement(82230..83660)
                     /locus_tag="ACGI68_000091"
     CDS             complement(82230..83660)
                     /locus_tag="ACGI68_000091"
                     /inference="COORDINATES: protein motif:HMM:TIGR01538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0136555.1"
                     /translation="MYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGEEYY
                     YGNADINNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNADDD
                     NLTKLLNDLLGEEFDDTITELYLNASNKGIEWLHPYINRKGEFKYVIIPAEEAIPIWD
                     SKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFIERGNSFIQEFLYDEYGKMT
                     DIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLNFYKSLIDIYDNNISTLADNLDEI
                     QEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRLEKN
                     IIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEYLKI
                     SGSKSYDYKSVQITFNHSMIINESEKIDMAAKSIGIISDETIVSNHPWVEDVNDELER
                     LKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            complement(83675..84928)
                     /locus_tag="ACGI68_000092"
     CDS             complement(83675..84928)
                     /locus_tag="ACGI68_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFH0136556.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYSKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFPTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANNKGLEKNLFMYCDSAEPDRIKMWKSAGY
                     EAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            complement(84906..85598)
                     /gene="terS"
                     /locus_tag="ACGI68_000093"
     CDS             complement(84906..85598)
                     /gene="terS"
                     /locus_tag="ACGI68_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFH0136557.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPKETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKGKEEMVKELKKYESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            complement(85668..86177)
                     /locus_tag="ACGI68_000094"
     CDS             complement(85668..86177)
                     /locus_tag="ACGI68_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136558.1"
                     /translation="MYKKVLNILLNYYKAINSKEVVELVLTPVLICIISYMFFNSSIN
                     KEVVISLNKDVLTLSGLLVAFGVCIITLLFTTYNKSISEAKDIKTERKVNGFNISYFQ
                     FIQLKAYYTVIMEILVVVICFINTIMLTRYYSNIIFYVLIFFTVHIILSLTTLIISMF
                     HLSWKDRGD"
     gene            complement(86198..87172)
                     /locus_tag="ACGI68_000095"
     CDS             complement(86198..87172)
                     /locus_tag="ACGI68_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136559.1"
                     /translation="MSNFKKRYIDVYRIDIINKINGSVYSDEEILKEVIKTFFKDKEY
                     CKENKNGINITKDKKFWITEVKCSEDDLIIKVKLEYTKYNQNTNIINAHTKEVEGNKK
                     MDQGDSNKQHLFIKFMKENNIAVVLFERVFVGVPMIELSKNLHLYYEDMIENNKFDNN
                     MSKTSINITQIASKDFIEQILDLDGVSKITLNVDREKFGTDEDNLFSNLNNSRRHNDL
                     IYKPTFRTRYLPSEVKRCCEKYIKGEEFRNKKINRIIIEGEKNKRKLKLDTEGIKLCR
                     EIEVSLDIDNHIDSEDIFDKMNNLIDDLIENNSVFFNLMYQEVAMSEA"
     gene            complement(87475..87711)
                     /locus_tag="ACGI68_000096"
     CDS             complement(87475..87711)
                     /locus_tag="ACGI68_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFH0136560.1"
                     /translation="MPNFGTTFVTEEAVYNMSFRSNKAEAKLFTKWVTKALKQIRIHG
                     YYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVY"
     gene            complement(88196..88675)
                     /locus_tag="ACGI68_000097"
     CDS             complement(88196..88675)
                     /locus_tag="ACGI68_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFH0136561.1"
                     /translation="MDKESIKEIVRELRKEEREDRKKGVLYNTRLLMKHYNDFKRHID
                     SAVSEAKDVDYLEDDLGKLDNEELYILSIKKSKARTIIMIAHIDSAMKTLKLRQERLH
                     SYEKYRALELYYCDEKTYEEIAEILNCGVVTSRRWINEMIKELGVYLFGIDGLKLTV"
     gene            complement(88668..88805)
                     /locus_tag="ACGI68_000098"
     CDS             complement(88668..88805)
                     /locus_tag="ACGI68_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136562.1"
                     /translation="MELISPGKIVRCAWCGRRFYKLDKSKVKYCSRSCSKRARRSDLS
                     G"
     gene            complement(88829..90199)
                     /locus_tag="ACGI68_000099"
     CDS             complement(88829..90199)
                     /locus_tag="ACGI68_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFH0136563.1"
                     /translation="MEFKPHPYQEYTIRKTIDNNNIGLLLDMGLGKTVCALTAISELM
                     YDYFDISKVLVIAPLRVARDTWSSEVKKWEHLKHLKVSKVLGSKLDRVRALSTDSDIY
                     IINREMVPWIVDFYKRKWPFDMVVIDELSSFKSNKAQRFKSLKKVLPLTKRVVGLTGT
                     PTPNSLIDLWAQMYLLDRGERLGKTITGYKERYFEPGQKNYQTGAIYNWQPKDGAENA
                     IHNKIKDICISLKAEDYLNMPKKIDNKIEIHLDSKILKYYKELEKEKILELEKDIITA
                     SSAAVAANKLLQLANGAIYDNDKNVKELHREKLEALKEIIDVSNGKPIIVFYNYKHDY
                     NRLMKEFKSLKPRTIENSKDIYDWNNGRIQLLLCHPASTGHGLNLQSGGSIIVWFGLT
                     WSLELYQQANARLYRQGQRETVIIHHLICKGTIDEQVMEALENKDKGQSALLEAVKAK
                     LKEYRE"
     gene            complement(90148..90474)
                     /locus_tag="ACGI68_000100"
     CDS             complement(90148..90474)
                     /locus_tag="ACGI68_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFH0136564.1"
                     /translation="MLESKIEKRLKKEIELLGGKAMKFISPGEAGVPDRIVLLPEGHV
                     IFVELKAPGKKLRKLQQYKMRELRELGFKVKCVSTLKEIDDFIKEVKGWNLNHIHTKN
                     TQLEKL"
     gene            complement(90647..90772)
                     /locus_tag="ACGI68_000101"
     CDS             complement(90647..90772)
                     /locus_tag="ACGI68_000101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136565.1"
                     /translation="MYVKVSVNRIKNRVNLKILKVSTMSTKKMALLTLMLTPKMQ"
     gene            complement(90756..93173)
                     /locus_tag="ACGI68_000102"
     CDS             complement(90756..93173)
                     /locus_tag="ACGI68_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFH0136566.1"
                     /translation="MDIKASEIEHINVRHDGQLMLAIGKNKLETHWKNKSILWSELVN
                     RLSKTLRTQETYTEYRKMSKTEKDRVKDVGGFVGGSLKNGRRKAENIANRSIITLDID
                     YANKDIWEDITLLNDYACLMYSTHSHTENNPRYRLVIPLTRPVLPEEYQAISRMIADT
                     IGIDMFDDTTYQPHRLMYFPSTSIDGDYIFKFQDGEFLNPNEILDLYLDWTDVSYWPE
                     SSRERQKFNTQLKKQQDPIEKAGIIGAFCRSYSIKETIETFLNEVYIPGIDETRYTYS
                     EGSTSGGVVIYDDKFSYSHHGTDPASGILCNAFDLVRIHKFGELDEDAKPETPVNRLP
                     SFTRMSEFASSDTKVRKTIGRENLDKAKDDFGDIDFEDDEWLTRLDYDNKGSYKKTTN
                     NILMFIENDPYLKGKIAYNEFSNRAVVLGKLPWRKDDKLNDWNDSDDSGLRHHIETIY
                     NISSPSKVNDALIIAFENNTFHPIKDYLNSLKWDGIKRVDTLLIDYLGAEDNHYTRTI
                     IRKVLVAAVARVFNPGIKFDNMMVLSGPQGMGKSTFIKKLGGDWYSDSLTTVQGKEAY
                     EQLQGVWLLEMGEMMATKKADIEAVKHFLSKSEDIYRVAYGKRTSRFLRQCVVIGTTN
                     DKEFLRDKTGNRRFWPIDAGVKKTNKSIFNGQLDNERNQIWAEAVELYKANEQLYLSD
                     EEKKEAERQQRTHSEENAKSGIIEEYLNKPITKNWYDLSISEKREYIHGSDFGDLKEG
                     TILREKTCVMEIWVELFNGEPKQLTPILSREINDILKGLDDWISYNGNLRFGKIYGRQ
                     RAYVRKS"
     gene            complement(93185..95143)
                     /locus_tag="ACGI68_000103"
     CDS             complement(93185..95143)
                     /locus_tag="ACGI68_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFH0136567.1"
                     /translation="MRTLSIDIETYSDLDIKKVGVYRYVDSANFEILLFAYAFDNEEV
                     KVIDLVNDEELPKEVIEALNDNKVIKSAFNANFERTSISKFLNINLKPNEWSCTMIKA
                     LTLGLPGSLDSVSKALKFNEDKQKMKEGKALIQYFCKPCKATKVNKGRTRNLPIHDME
                     KWNKFKEYCKQDVVVEREIRNKLSKYKTTEREIKLWYLDQRINDTGIKVDTELIENAI
                     ECDKRYTEKLTKEAIKITGLNNPNSPAQLKKWLSDKVGFEITSLTKESIPEILKQVDD
                     ENVVRILELRKLMSKTSIKKYEAMKLAKGNDNRVRGLLQFYGANRTGRWAGRLVQVQN
                     LPQNHIEDLDLARNLLKEGDFDLIELLYDSVPDVLSQLIRTAFIPSEGHRFIVSDFSA
                     IEARVIAWLAGEKWRLDVFNSHGKIYEASASQMFKIPIENIKKGSELRQKGKLAELSN
                     GYGGSVGALISMGAIKMGLKEEELQPIVTAWRNANPNITKFWWDVDKAAKKAIKDRTI
                     VEIQHGIKFIYNPGVLFIELPSSRRLSYLRPKIEPHTTFSGDKITYEGMEQTSKQWKR
                     IDTYGPKLVENIVQATARDCLREAMFNVTDAGYSIVMHVHDELVIDVDKKGGSLEEVN
                     SIMGKEISWAKGLPLKADGYECDYYKKD"
     gene            complement(95143..95715)
                     /locus_tag="ACGI68_000104"
     CDS             complement(95143..95715)
                     /locus_tag="ACGI68_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFH0136568.1"
                     /translation="MSNSVQSTKVVTGKVRLSYCNIFKSRAMVEGAEPKYSVCILIPK
                     SDKVTLGRIKKAIDAAKEQGKTSKWGGKLPGNLKTPLRDGDAERADEAEEYVGMYFLN
                     ANSTQKPGIVDKDLNEILDNTEVYSGCYGRVSINFFPYNSAGNKGIGCGLQNVQKLAD
                     GEVLGGARASAEADFSDDFEYEDEEEDFLS"
     gene            complement(95738..96310)
                     /locus_tag="ACGI68_000105"
     CDS             complement(95738..96310)
                     /locus_tag="ACGI68_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HFH0136569.1"
                     /translation="MSNQEITKLVENNLNLVHFSINRFFKSYVEQHPYLYEEFYQEGC
                     IGLYKAALNYDSNRGKFSTVAVSYIRIEMFKALRYGEKHYRNSIEGLDVSVFEDSKRP
                     LKDIHGFYEIQFNYDVDYIRKIARKSYLKDIDKIVDWILQDKKISDIGELLNVSSTVI
                     CGRIKLFSKQVRDLDKFGEITSNIKSIYQI"
     gene            complement(96363..96698)
                     /locus_tag="ACGI68_000106"
     CDS             complement(96363..96698)
                     /locus_tag="ACGI68_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136570.1"
                     /translation="MPLDAGIKDKLDESISIILDLKDRKKSIEKDLDYEKDNLLDMLK
                     ILNITEYNSTEENAKVIVMDFKRESLIKDKVMSTFLEVNDNHLDRVYIPEHIKVSPVC
                     FVSVRAKDN"
     gene            complement(96713..97879)
                     /locus_tag="ACGI68_000107"
     CDS             complement(96713..97879)
                     /locus_tag="ACGI68_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFH0136571.1"
                     /translation="MPLQHARLSASGAHRWLHCTPSIKLEENYPPSTSIYAEEGTVAH
                     ELAEVKLMLEYEKISKKAYNARIKKIQKSEYYNSEMEDYIQSYVENVVELVNDSKAIC
                     DDVIVMLEERLDFSEWVPEGFGTGDVVVISDGILQVIDLKYGKGLEVSAIENPQLRLY
                     GLGAYNQFEMLYDIDLIKTTIIQPRLDNISSEEIEVTKLLTWADNVKKKAQMAFNGEG
                     EFVSGSHCGFCRAKNDCRKRAEDNLKLARKYDFADTFALNKYEIADILGFAKNIQDWL
                     KDVQSYALEQAEKHGVKYPGYKLVEGRSNRKYVDEQEVAKVLLNSDYDEEKIYKPRTL
                     KGISDMEKAIGKKSFAKLLSDLIIKPVGKATLVVESDKRSEINSIDSAKKDFEI"
     gene            complement(97891..98328)
                     /locus_tag="ACGI68_000108"
     CDS             complement(97891..98328)
                     /locus_tag="ACGI68_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136572.1"
                     /translation="MVEVNVNVKVKVEAPEFTNALLVVANALGGLNLGKAMQIEPINI
                     TDMKEEKKVEVKKAEKIKKVEVKEDVKEEIAEAKEEIEKNNENTTSEVKYTKEEVRTK
                     AAQVSKAGKKDKLKELFGEFGASKLSEVKEEDYSAFMNKLESL"
     gene            complement(98399..98548)
                     /locus_tag="ACGI68_000109"
     CDS             complement(98399..98548)
                     /locus_tag="ACGI68_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136573.1"
                     /translation="MKLLSLDTNNKNVVTLKKDGKVIADIVFKDIKTGKKISVGILNK
                     KVLVK"
     gene            complement(98529..98921)
                     /locus_tag="ACGI68_000110"
     CDS             complement(98529..98921)
                     /locus_tag="ACGI68_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0136574.1"
                     /translation="MDISESITKQFSDSLKSLIEIEISKQETDRVKSQTIEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTAFMRRLVSTGLIKSLVRGDGRKISRYEV
                     DDFIERNQGKNLDELLKAVERGDEIAES"
     gene            complement(98952..99149)
                     /locus_tag="ACGI68_000111"
     CDS             complement(98952..99149)
                     /locus_tag="ACGI68_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0136575.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELDIDVSGLYKLLRNKSSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            99225..99599
                     /locus_tag="ACGI68_000112"
     CDS             99225..99599
                     /locus_tag="ACGI68_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6173 family protein"
                     /protein_id="HFH0136576.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            complement(99609..99818)
                     /locus_tag="ACGI68_000113"
     CDS             complement(99609..99818)
                     /locus_tag="ACGI68_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136577.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMSKSEKRKISEITKERIRK"
     gene            complement(99865..100680)
                     /locus_tag="ACGI68_000114"
     CDS             complement(99865..100680)
                     /locus_tag="ACGI68_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFH0136578.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSNGMPDVKMTTKWTQKGRLFLYDLLKTNNILPDIEKEYSYQTSILG"
     gene            complement(100683..100904)
                     /locus_tag="ACGI68_000115"
     CDS             complement(100683..100904)
                     /locus_tag="ACGI68_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0136579.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            101057..101620
                     /locus_tag="ACGI68_000116"
     CDS             101057..101620
                     /locus_tag="ACGI68_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0136580.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            101644..103233
                     /locus_tag="ACGI68_000117"
     CDS             101644..103233
                     /locus_tag="ACGI68_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0136581.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IAFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            103496..104002
                     /locus_tag="ACGI68_000118"
     CDS             103496..104002
                     /locus_tag="ACGI68_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFH0136582.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            104242..105372
                     /locus_tag="ACGI68_000119"
     CDS             104242..105372
                     /locus_tag="ACGI68_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0136583.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKEDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITVEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLNNKTITVRHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
BASE COUNT        33853 a        18555 c        11275 g        41872 t
ORIGIN      
        1 gtgtctcatg agctaaaaag tttattttga gacactccct tacttatact tatctgtgat
       61 acatataaaa gtttaaagtt ctaaaattaa ttctaacact taatttgcaa caaaaaataa
      121 cttttcttgt aaacttatat ctccatttat tagctcagct tcataaatat tctttttaag
      181 tttcatatca aatacttgaa attttactgg cttagttccg actacatcaa taatcccttt
      241 aaatcctttt aattctcctg atatactgtt cttaatacct gaaatagaat atatcttatc
      301 atccagtagc ttaactttcc taactccaga tgatgcttca ttataatcta cttttaaatc
      361 aagtatctca taatcttcta atttaatctc attagaatta atatcataaa caagattagt
      421 tatggaatta ctcatatccg taactataag attcaattta ttgtttttta ccatataatc
      481 tagcttagtt ttacttaata agccaatctc ctttggattt gcattctttt ctttgtttag
      541 attatccata aatttattac ttatattaaa agtgttatct tctatatcat atataataaa
      601 gttagagtaa ctatcatgtc cttcaatata tccattttta cttttattat ttactatttc
      661 taagtataat ttacttccaa tagaaaattt aatttcattg atattttgtt gttctccatt
      721 ctgtaatcct aaattaaatc tatttaataa tttataagac ttatttttta tatctatctt
      781 taatatacaa atattattga ctttagaacc tgaaatatta gtttcactaa ttaccatata
      841 taaattacct ttatatattt tgttaaataa ggttttccaa ctattttctt taaaatcaga
      901 cttatttaaa ttaatatcaa aattttcttc taccccactt tttaaatttt tatttgaaac
      961 ctttatacaa tcatttaaag cattcattcc ttgtactaaa tcagacttaa cagaggtata
     1021 cgcttctcct atattcaaat catctttata tataaattct tttcctttat cattaacaaa
     1081 gtcactgtat tttttgtcaa tatttaaata atcatttcta attcttgatt ttgggtttgt
     1141 attatttctt actccatttt tacttataat tacctcctca taagaaaatt tgcttatatt
     1201 aggtgtatat actacactta ctctccctat atcttttaca ttgcctttta aagtttttac
     1261 agatagttta tccttattaa aataactcaa tattaaggtc atagtaatca ttaaaaaaca
     1321 tactattata aaacatattg tatattttcc tatatttttt gctttcataa tatccctcct
     1381 aaaagtcaat cttttgcttc aaaagtttat tagatataag actacataca aatatgttta
     1441 tacttgaata tagaacagta aatacatcac tgtattcgta aggtataata aattctatag
     1501 aaattataaa cactaaaatt tctataacta aaaatagaaa acttattaaa gtgcttactt
     1561 tataaataga tttatttata agaattgatg taaatactac agatacaaat cctatcaata
     1621 aaaatatata ctctacaaat agtatttcaa agctatatgg aagtaacatt tttaactcat
     1681 atattgtttc ttgtacaaaa ccatagtttg tgacattacc aagcatatgt ctaggcaaca
     1741 acttacttgc aataaacaaa gatattgtaa gtactactgt atagctatac actagaaata
     1801 atatatttaa taatttagat atataaatat tgattctatt tttaggtagc attataagtg
     1861 tatatataga tttacttcta ccattaaagt ctctatacca aataaaaaaa gcatagcaaa
     1921 tacatgcaat tataccaaac ataaaccacg attctgtaga acccattata tttctaaagc
     1981 ttgagaaacc tgtaacattt accgtttcta tcgtatttcc gatttttgtt aactgttcac
     2041 ttttaactgc attttctatt atgtaattat agtcatttaa attttttact atgtttaaaa
     2101 ttacaaaaga acaacaacaa attattataa atatataatt ctttatattt ctttttaatt
     2161 caaattcgaa taatcttatt aaatctttca tcaactcacc tactttattt caaattctaa
     2221 catactattt gaactgttaa attgtaattc tccttcatat acaatttcct tagaattttt
     2281 atttattata tatagcataa atttatcatc ccaagataat atagagttta gccttgatgt
     2341 atatgcaacg attttatttg aatcaatttc aatacttcta attcctcttt tttctctatt
     2401 atctaaaaca tatttatctt catctaagct tttatctaat atattatagg taagtaaatt
     2461 taactctgta attgaactat tatcataatc attcgacatt accatatata atttattagc
     2521 ttctaacttt gcttttgcta aagccatttt gctatcagtt tttaattcca ttatgctttc
     2581 cttattttct tcaagattat actcaagtaa atgaaattta gaattactat tatcacctat
     2641 gtttgtattt agtacattta ctaaaatata catcttatcc tcatatttgc tagcataact
     2701 tacacctatg ttctctatgt tttggtattt ttcggaaaaa taattactta aatttatact
     2761 ttttatagtc ttgatttttt tattttctgg attaattttt attatatcta tagtacttga
     2821 atattctttg ttattttctg atgaatcact tgtagcataa agtacagata catataagta
     2881 ttttttatat ctttttgatg ctagtatctt tgcataataa tcatctgtac ttttatctac
     2941 tttatatctt gtccttgtaa aattattcat atctaatttt tcatattcaa tttctttatc
     3001 actaccttct gatggaaata cacctttttc taaatcttct tttttctcat ctatcacaaa
     3061 gtttatatct tttgactcct cattcatttc aaatccaaca tgagcaatta aatctgaacc
     3121 actagataca tcaattaaat tatttgtgtt cttactatcc ttttgtagtt ttattaattc
     3181 agtctttttg gttttgttgc ttccatttat tgtataatat gaatttatat atccattcaa
     3241 agcatatcca aaatttatgt ttatatggtc taattcatta gagtcacctt ttatagtttt
     3301 tatctttaaa ttatcttcat tatttaataa acctatacta aaatatattg ctaaagtcaa
     3361 aaatataata aatatagata ttttagattt taatttcata ccacccctcc ttaaagagat
     3421 actttctttt taattagcaa ataagatatt ccatttagaa taaacattat aattaaaaaa
     3481 cttaataacc ctgctactaa attgttaaat tgaaatccta taagattata atattttcta
     3541 aataaagtga ttatatttat atatattgtt gaaaaatata agcctgctat aatcagcaaa
     3601 gtgattaaaa ttacataaaa tttatttata taggaaaaga taaataatat acagcaattt
     3661 acaatagaaa tagctataag tagtaaaaat acataaatag ccataaaaga gataaaatca
     3721 attggtatac tcttcaagtt taaataatat aaatctttgg caaagttcat attgattata
     3781 ggcatatttc taaaaatgaa attaaatata tatttacata taaataatgt tgctgtctga
     3841 cttatcaaaa aaccatatat cattgttatc aaggctattg atttagatat acaaatctta
     3901 aacttatttt gaggtaacat cataagtgta taaatacttt gattatttga aaagaactct
     3961 cttttccaca taaaaatata gtatataaaa agtcccaata tcccaaagaa caatatactt
     4021 tggataaaac caagattact aagtatccct agtccataaa atatacctcc aaaatcttct
     4081 attgctaagt tcataataaa tttagaattt ttaaatttat taatttcgaa taaatttaaa
     4141 ctaagtgtaa ttaatgaaaa aaatgatata taagctatat aaaacttatt gcttcttctc
     4201 aaatctacat ttattagata tagaagttta ttcattctta tatacctccc tcatcttatc
     4261 taatatagat ttaccttctc tttctcttaa ttcttctgca ttaaatgaag ttataatctg
     4321 cccttcatca agaattacaa cctcatcaac taaactttct atctctacta tctcatgagt
     4381 agttataata atggcttgat tttcctccat atattgcgct attactccta taaactcttc
     4441 cctcttaaac aaatcaattc ctgtaaatgg ttcatcaaga agtatgtact ctgggtcttg
     4501 gcaaaatcca agtattaatt ttactcttgc aatgtttcct tttgacaagt tatcaattat
     4561 ttcatcttca tccaatttaa atatgtccat catttctttt gatttttctt tattccactt
     4621 tgaataaaaa atttccataa attcaaatgt ttcttttatt gttgtattgc taaagtgaga
     4681 ttgtacatct ggaacaaaag ccaatttatt gtataccttt tcatttattt tttctccatc
     4741 tatacgaatt tctccactat caatgtttat aagtcctgct attgctttta acatagttga
     4801 tttaccaaca ccatttatac caagtaatgc agttattttt cccttttcta tattaaaact
     4861 tacatttttt aaagcttcta tctttcttat tttaagccct tgcatccttt tatatgtttt
     4921 gctgacattc tttatttcta tcatatttac tatcctccac atccaatatg cttataaata
     4981 aactttctaa actaagtttt ctatttgtta aaaaactaat acttatcttc tattattttt
     5041 aaaacttctt ctttgctaac atttattgct ttcatatctt ctataaaaac agatagtgat
     5101 tcatttataa gctctaattt taaatttttt aatatatttt catcaactgt tacactgctt
     5161 tgataatttt taaaagtatt tattatattc atatcaccca tctccttata tgctctttga
     5221 actgtattta agtttacttt tagattaagt gccatttctc ttcttgatgg tattgtctct
     5281 cctggtttca actctcctgt tactatttgt ctctttatat actttactat ttgcaaataa
     5341 atgggagttg tattatctaa ttcaaaattc atgattcctc ctattctagt gtattaatac
     5401 gatagtacac ttactttaaa aaaatacgcc tttatttcag actttatcta gtatcctatt
     5461 aagtgtatta tacgattaat acacttaatt tgtaaatact tattttataa attgctttat
     5521 tttaatttgc taaaaaatct aaagtaaagc ttaaataata actttacaaa atggagcatc
     5581 aaatgagagg atatatattt tatgttatgt tagacttgaa aacaagcaaa cgagataaga
     5641 ttatatgttt tcaactaatc aaaatcttat atgtattaat gcttaaaaat ctagtaagaa
     5701 tatgaacaca cattaggagg taaacaaatg aattgcaaaa ttgaaatcaa aaatatcgaa
     5761 tcagtcacag tggctaccat gcgttataat ggacctatga ctgaggcaac aaagtatttt
     5821 ccaaatgtat ttaaagctat aaaaggaaag tctaacggtg caccattctt ttgctactat
     5881 gatgttgacc aaaaaactgg tgttgcagaa atggaattat gtgttccaac tgctgaaact
     5941 ccaaatcgta tgggtataac tacaaaagaa tttcctaaaa caaaagcttt attttttact
     6001 cacacaggct cttatagtaa tttgccaaaa acgtatgaaa tgatatttaa atatattcat
     6061 gaaaataata taaaaataca aactccttgg agagaagtat ttattaaagg accaggtatg
     6121 ttgataaaag gaaacccaga taactatatt acagagataa tatttccact taaagaggag
     6181 gaataatata tgttagcgat tgaaatagac aatttagtaa aagaatataa aaatggtgtg
     6241 aaagctttaa atggtctaag ctttaatgta aatgctggag aaattttttc tttacttggt
     6301 cctaatggtg ctggtaaatc ttctcttata aatattttga ctacctttta caaacctaca
     6361 tctggaaatg taacaatgtt tggaaaagat ttagtagata atccttcttg gatacgtact
     6421 caaattgctt gtgtagcaca gcaaatctct attgatgaac atttatccct tatggaaaat
     6481 atggtatttc aaagtaaaat gtataaagta gaaccacaga ttgcaaaaca aagaatagac
     6541 tctttaattg ataagtttga cttatctagt tacttaaaat atcctacttc atcatattca
     6601 gggggtgtaa aacgtagatt ggacatagct atgaatatgg tatcatctcc taaaatccta
     6661 tttttagatg aaccaactgt tggtatggat gtcgattcaa gaaaatctat gtgggatatg
     6721 ttattaaaaa ttagagatga gtatggcaca actatatttc taactactca ttatcttgaa
     6781 gaagctgaac aattgagtga taatatttgt attatgaaga atggaaagga tttagcacaa
     6841 ggaacaccat caagtttacg tagttacatt agacaaaata tactacgtat aacttttcat
     6901 aatactgaag atataaaaaa atataaagat tcaattaaaa gtactggttt agtaaagttt
     6961 atgagcgtac gagaaaattc aatttttata agtgtaaacg atagtagaac tgcttttact
     7021 ttaataaata aatggctttt agaacatgat atagaatttg atgcaataga aattgtagag
     7081 ccaagtctcg aagatgtatt tttagcactt acaagttcta aaaaaagctt aaaggaggaa
     7141 tgggaatgtt aaatatctta tggcgtagta tgaagtggcg ttttaaaaat ccaatctcat
     7201 ttgtagttac tatattacaa ccttttcttt ggcttgtgct ttatagttct attgcaaacc
     7261 aaactatgaa taatataaat atcaataatt atactgcttt tatactacca ggtatcattg
     7321 tactggttgt gttttcttct tgtagtagtg gtggtattat aaactttata atgaagaaca
     7381 gtggaagttt ctatagagtc ttaattgcac ctatcagcag gtattcaatc gtccttggtc
     7441 aattattaga agctatctta gtttctttca tagaagtaac tattttgtgt atagtaagta
     7501 tattcttctc agtaaggata gaatctggca ttggtggaat cctacttatg atagtactga
     7561 tatttatgac agccttcttt ttatcaagtc tagcttattc tataagttta ttattgccaa
     7621 atgaaatagt ttatgaaaca attatgactg caattgtact tcctattttc tttttaagtt
     7681 cagctctttt ccctatagaa agtttatctg gaggattaaa agtagcagtg atgctcaatc
     7741 catttaccca tgttattaac gctttacgta gcttaatatt tggtgagact attcttattg
     7801 gagatataat gcctgttatc ctactatttt taatattgtg ctgttcaagt ttttcacttg
     7861 ctatgtggag attaaaaaaa gaaatggtaa gctaagtttt aagcctatta ataacaaata
     7921 ataaaaatgg actctattat atagaatcca cttttattat ttattttaat atttctaaaa
     7981 ccgaatttaa cacagcatga ccattacaag tgccatagtt taccatatct attatagata
     8041 ctggagctct atctccaact atagacttta cgtcatcaag cataaatcta agttgtggtc
     8101 caagaagtaa aatatcaaag ttatcaagct ccgattttat atctccttct ccaactgccc
     8161 atattttaca gtctatattt cttttttttg catccttctc cattttattt accaataggg
     8221 tagaagacat gcctccaaca cacactaaca atatcctaat catttatagc tcctttttaa
     8281 ccaaatctac atatatcttg attaaaaagt atatgtagaa aagtttatca tttataattg
     8341 cttaaatttt actcttagat tatatcaaat ttttccattt ttttcactac cagagccaat
     8401 tttatatttt taatgtccac ttttccactt ctttagagat atcttcagct tttaatatag
     8461 ctgatattat agcatacccg tcactttcta aacatttaag tttatctata ttgtctaaat
     8521 ttatacctcc aattagtaca aatggcatgt ctactttttc tttaatttct ttaacagttt
     8581 caaacgaagc tgattttgca tctaatttag tagatgttgt gaatatagac cctacaccta
     8641 aatagtctgc gccatcattt tttgcacaaa tagcttcttc taaagttctt gctgatactc
     8701 ctagaatttt attgtttcct ataagctttc taacctctct aacatcaatg tcactctgtc
     8761 ctacatgtac tccatctgca tcacaaataa gtgctatatc cactctatca tttattataa
     8821 acttaacacc atatctttta gttatttctc taagtttcat agcttttctt aaaaactctc
     8881 ttccagaagc atttttttct ctaagttgta cagttgttat tccagaagat attgcatctt
     8941 caagacattt ataaaagtct ctacctttaa gcatttcaga gtcagttact aaatacaact
     9001 ttaaacattt ttttaaagat tctttatcaa tcattttaag tctcccttac tctttattaa
     9061 tttaaaaatt caatattaga atttttactt aaagcatcta tatccatttg ataaatagca
     9121 ttcataagat tttctttaaa tgtacctatt ggtggattat ttaaattagc taattcccca
     9181 cttaaagaca ttgtaagaac acccattgtt gctgcttcta atatattctc agtaacagcc
     9241 ataaaacttg caatcaagct agcagtcata catccagttc ctgtaattcc ttttaattta
     9301 tcagttccat tgtttatttt atatgtacct ttaccattag ttattatatc tatttttcca
     9361 gttgctacaa ctacacattc aagcttttca gcaatctttc ttattatctc agtagaatct
     9421 tcttcttcat caaatgaatc tacaccttta ccccttacat taaagccacc tataaacttt
     9481 atttctgata cattcccttt aacaacacta aatttgactt catttaaaag tctacttgta
     9541 agttctgctc tcgctttgct tgcaaatact ccaactgggt ccaaaacaac aggtttattg
     9601 tacttatttg ctgcttttcc agctaatagg aataaatcca acatatttga gttcatagtt
     9661 cctatattta ttactacaga gtttgctaca cttaccattt cttctacttc ctcatatgaa
     9721 aaactcataa gaggacttgc tcctacagct aaagttacat tagcacaatc atttatagtt
     9781 acattattag tataatgaat taccaatgga tttaattttt ttacatcttt aattaaatta
     9841 tacatattct acctccaata tttttttatt actcaaattt ataaaaatga tgtactggtc
     9901 ctactccatg acctatatca aaacttcttt ttattgcttc tgttatatag attttactta
     9961 gatttactgc ttctgttatt tcatatccta atgctagata agatgttatt gcagaagata
    10021 atgtgcaccc tgttccatgt gtattttttt tatcaattct ttcactttta taaactttaa
    10081 atatattttt accaactaaa atatccactg cttctccatc taaatgacct cctttcataa
    10141 gtacaaattt aggtccaagt tgtagtattt cttctcctac tctcttcata tcatctacat
    10201 tatgtatttt tattcctgtt atttcttctg cctctggtat atttggtgta attatgtatg
    10261 caagtggtat caaatactta attaagtttt cttttgcctc tggctttaat agataatatc
    10321 cacttttaga tatcattact gggtccacaa ccaaatattt tggattgtat ttctttagat
    10381 tctcaactat ttcaagtata atctcaggac ttgaaaccat tcctattttt acagctcttg
    10441 gaggtatatc ttcaaagact gcctctattt gtttttttat tattttctta tttaaatcct
    10501 ctacagcaaa aactccttta gtattctgtg ctgttatagc tgttattaca ctcataccat
    10561 aagtaccaat tgcactaaac gttttcaaat cagcctgaat cccagctcct ccagatgaat
    10621 cagagccggc tattgtaagt gttggtattt tataattact cattgttgcc tcctaaagtt
    10681 taatttaaat aacttaatta aaaaagctat acctacaata ggtatagctt agaaatagca
    10741 tatatcacaa ataatacata cttgaatttt tactaagctt tcctacgctg gtattatcca
    10801 gatcaggtgt aagggtcggt atacctctca gctcttggct cccctagcta ttttaattta
    10861 ctatatttat aacttcatta tattacaata gtatttattg tcaagttttt acaccatatg
    10921 cacccattat aatgttacta taaatattta tgttaattgt tctatttcct taccaataat
    10981 ataatcttct ttattatcaa ttaaatttat aagtatactt gggtttttta ttatagacaa
    11041 ttttttattt ctagttaatt tttttatata gtattctaac ttcatagcat tgctttttgt
    11101 gttggtcaca aaataaactt ctagtttttc aaatcccttt gctcttgtgt attttgaatt
    11161 aattcctaat ttatgttcat tcattcttct aactatatta ctggtatatc cagtgtatag
    11221 agaatcatct ttacatctta taatatatgt aaaatacacc ttaaatcaca tcctaacatt
    11281 aaatatataa atattatatt atagaattaa aaaaaatggt gtgtattttg aatatgcaat
    11341 cttcaaagaa ttaattataa tatatgtata aaacatactc tacatgaatc taatgctaaa
    11401 ttttgacata ataataacat atttatttct ataataaaaa attcacaaat tataaaaaat
    11461 aaaattttca aaaaaacaaa ttgacacata taaaaaaatt atgataccct aataaaaagg
    11521 gggcaatgat tatggaaaac aatttaaata cagctaaaag aaataatact gaattattac
    11581 aaaatgactc acttaaggtc aacttacaga taggtgtaga ctttacactt ttatcaaaat
    11641 cacttaaagc cttttataaa aaagaaaaag atttttattg cattgctctt gcgccatgca
    11701 atgtatctag tgaaaaagca aatagaaaaa taagtattta cgatatgata aaagaaatta
    11761 atattttaat tgaaagtata actgataaaa atgaactttt aagtgaagaa ctctttacta
    11821 ataatttatc agaattttat gacgaatctt tagatgagat ttatatagac ttaaaacaag
    11881 cgtatttata tattaaaaag aatacagatg gtcttaacat agaagactca attgaatatg
    11941 tatttgatgt agatataagc aatgatataa aagcaaaaga aaatccacta attaatttta
    12001 attcactttc tttagtagta tggagtggcg aaacaaaaca tattttacaa caaatgaata
    12061 ttttagattt agaggatatt cactcttatt ctatagaaaa taatacaaat ataactatct
    12121 atgaaaataa tactcttcaa tataagtttg atgaagaatc tgaaaatata aaagacagta
    12181 gtatagatat aattgaacaa atgcttatac ttcctgatga cttgaattat gataaagatg
    12241 aagttgaaaa tatgaaaaac agacttgcaa aaataaatat taaatattta cagacactta
    12301 aagaaaagga tataaaaata aaattaataa attcaaatct aactgatgaa ccagaattta
    12361 gtgatttaaa atatcaatta cctccatgtt gggtaagatc aggcaagact tggaaagatg
    12421 tacctggtat atatagaaat aattctatag tggctaaaat aggttatagt aaccctagct
    12481 atgcaaatgt acattcatca aaaaatttag aattacatga aactgcacat gctatagata
    12541 aaaatgtttt aaataaaaaa tcaaattctg aagaatttat ggaagtattt gctcaggaaa
    12601 gatataagct ttatgaccct aagcaagttg cacatgcata tataagcaag tttatagaag
    12661 aattttttgc agaatcattt gtacattatt atcttgatga agattcaaaa aatactttaa
    12721 aggaaaattg tcctctaaca tatgattttc ttgaaaaact tgaactaaac tattaagtgc
    12781 gctaatatcc aataatacat ttaactatcc tcttttcatc aacaataacc atataataat
    12841 ttatactttg aataaatcac tatctataaa ggatttctta gtatttatgt tataatattt
    12901 ataatggtta tgttttaaaa tagtttatgt aacccataat ttaaataaat aataaaagaa
    12961 ggatgaaaag ttatgaagtt aaatagaaat gatagctgtt ggtgtggaag tactaaaaaa
    13021 tataaaaaat gtcatatgga tatggataat aaaatagatt catttgctaa gttaggtcat
    13081 attgtaccaa ctcatgatat tataaaaaat gaaagtcaaa ttgaggaaat aaaaaaaagt
    13141 gctgtaataa acacaaaaat tttggattat atttcagata aaatcgaaat tggaatgtct
    13201 acagaagata taaataagtt ggttcatgac tttacactac aaaatggagc tatccctgct
    13261 ccactagact ataatggatt tcctaaaagt gtatgtacat cagtaaataa tgaagtttgt
    13321 catggtattc caagtaaaga cattatctta aaagatggag atattataaa tgtagatgtt
    13381 tctactattt ttaatggata ctattcagat gcttcaagaa tgtttatgat aggtaatgtt
    13441 aatgaaaatg caaaaaaatt agttcaagtt acaaaggaat gtgtccaaaa aggtcttgaa
    13501 gttgccaaac catggggatt tttaggagat ataggtgatg ctatcaactc ttatgcaaag
    13561 aaaaatggat actctgttgt tagagaaata ggtggtcatg gagttggatt aagctttcat
    13621 gaagaccctt ttgtaagtta tgtatctaaa aaagggactg agatgctatt ggttcctggg
    13681 atgatattca caatagaacc tatggtaaat gaaggaactc acgaaatttt tgttgatgaa
    13741 gaaaataatt ggactattta tacaaaagat ggtaaattat ctgctcaatg ggaatgtatg
    13801 gttttagtta cagaaacagg tgttgaaata ctaactgaat aaaagacaaa tgcctataaa
    13861 aattgaaaat tcaattttta taggcattta ttttattaat gcacatctat tgcatcaact
    13921 ggacagcttt ctctagcttc tgctgctgat tcttcagcat cacttggtac ttcatcaact
    13981 ataacatgtg atttcccatc atccttcata tcaaatactt ctggacatat tgatggacat
    14041 aatccacaac ctatacaagt atcttgatta acatttgctt tcataataaa atcctcctag
    14101 acgctacaaa tttaaaatct tgtttatcaa agattgatga accaaaactg taacattcag
    14161 ttctttttta ttatgtcctc aaatttgaaa aaaatacctt ttaaaataat ttttataaag
    14221 tttattttat agttatataa atcctatata taatatacat tatctaattc atcatactca
    14281 taggacactt cttttttatt cttttttaca ggttctaggt ttcttatttt tactattttt
    14341 gctggtatac caacaactgt tgcaccatta ggtacatcct taacgacaac agaatttgca
    14401 ccaatttttg aatttgaacc tatatttaaa ggtcctagta tttttgttcc agcaccaatt
    14461 aatacatcat caccaacagt tggatgtctc tttccagtat cttttcctgt agctcctaaa
    14521 gtagctcctt gatatattgt caccctattc cctacttcag ctgtctctcc aattacaaca
    14581 cccattccat ggtctatcaa tataccttcc cccattttag caccaggatg tatttctatg
    14641 ccagtcataa atctggatat ctgggatatt aatcttgctg taaaaagctt cttcttctta
    14701 taaagagcat gagccatcct atgcattatc attgcatgaa ctgatggata aagtaaaaaa
    14761 acttctatct tagacctagc tgctggatca ttcttcatta tatattcaat atctttattt
    14821 attttcttaa acaaagtatt cactccctca aataaaatta ttaaaatatt cccatagaca
    14881 tatacttatc aactccatct ggagctactg taactatctt tttatctgga tacttttttg
    14941 ccatctctat agcaccaaat atatttgctc cagaagatat ccctactaaa attccctctt
    15001 ttgctgcaag taatttaact gtatcaaatg catcttcatc atctactaat attacttcat
    15061 caactacact agaatcataa ttttcaggaa caaagttagc acctattcct tgtattttat
    15121 gaggtccagt cttgttttct gatatagctg gagatttttt tggttcaagt gcaattactc
    15181 ttatatttgg attttgttct tttaaatatc ttgcagttcc tattaaagta ccacctgttc
    15241 caactccaca tacgaatata tctaaatctt tgacatcatt cataatttca actgctgttg
    15301 ttttataatg tgcatttgga ttatctttat tttcaaattg ttttaggctt ttatagtttg
    15361 gattttcttt taaaagttca tttgcttttt ctatggaacc tgccattcca gctttaccat
    15421 ctgtaagcac caaatttgct ccataagcct tcattaaatc tcttctctca acgctcattg
    15481 tttcgggcat tacgattact acttcatatc cttttaattt tcctatcata gataatgcaa
    15541 taccagtatt tccactagtt gcttcaacta gtacatctcc cttttttaat tctcctcttt
    15601 gctctaagcc ctctaacatt tcataaacag ctctatcttt tatacttcct gctggattta
    15661 ctttttctaa ttttacatat atgttagaac acccattagt acagcctaaa ttattcagcc
    15721 ttactacagg tgtgtttcct atcaactcta atgcgttatt atataacata ctatttcctc
    15781 ctatatgtcc aaatctatta tcaaattgat aacgataaat atattatagt attatcattt
    15841 tgataatgca accttttttt ggattttata cttacaatca ctttattcca aatattagta
    15901 atattcaaat aaaaaagtga aaatcattat cgattttcac tctttaaaat ttaaccttta
    15961 aattaaatct tgcgtaatat tttgttgcta aaattccaca aaatactata taagacctaa
    16021 tatttttgcg acctctacat taacttttaa tacattaact ctatcttcac ctaaaactcc
    16081 aaaaagtttt ttggaagtat tatcatctac aattttttgt aatttacttt ctgatattcc
    16141 agacgctttt gctatagctg gtatctgaat ttttgcagag gctggactta tattagggtc
    16201 aagtcctgaa cctgatgctg taagtaaatc tgttggtata tcctctcttt tgacagttgg
    16261 attatccttt aaaaactttt ctatatcttt ttgtactcta tcatgaagtt ctggatttgt
    16321 agctccataa ttaaaagacc ctgatgatac acctccataa gatgtatttc catctttatc
    16381 tggtactaaa tcttcttttg tataagtatt atagtttact gatgatactc ttcccttaaa
    16441 gaatctcgca tctgtaaagc tttgaccaat aagttctgaa cctactttta caccatttac
    16501 ttctatcata cttccattgg ctttatcttt aaatgcaact tgacttacac ctgtcaaaac
    16561 aagtggatat acaagtccac aaacaactaa caatacaata cttacaagta atgctggctt
    16621 taatgttttc atttttatat cctccattta ttaacctaaa ttaagaacca ttactaaagg
    16681 tgtaatcaat aaatcaatta attttattcc taaaaatggt actataactc caccaagacc
    16741 aaatataagc atatttctca ttaacatagc ttgtgatttc ataggtctat attttactcc
    16801 cttcattgca agaggtatta aacatgggat aattatagca ttaaatatta aagctgataa
    16861 tatcgcacta aatgacgtag aaagtttcat tacatttaat atctccattt gaggtattgc
    16921 aagagtaaat atagctggta taatcgcaaa atattttgca acatcatttg ctatactaaa
    16981 tgttgtcaaa gccccacgag ttattaaaag ctgttttcct atttcaacaa cttccaatat
    17041 cttagttggg tctgaatcta aatctaccat atttgctgct tcttttgcag atgttgtacc
    17101 actattcatt gcaagaccta catctgcctg agcaagtgca ggagcatcat ttgttccatc
    17161 acctgtcatt gcaactattt taccctctaa ttgttctttt tttattgcat ctattttatc
    17221 ttctggttta cattctgcaa taaatccatc tactccagct tctcttgcaa ttgtagctgc
    17281 tgtcaaggga ttatcccctg tacacattat tgtctttatt cctatctctc gaagtctagc
    17341 aaatctttct acaagccctg attttactgt atcttttaag tagataattc catatatctc
    17401 attatctaca cacactacta aaggtgttcc tcctagttta gctactcgat ttacattttc
    17461 ttctaaatct tctggcactg taccaccaga ttcttttact ctttttatta tgctatcata
    17521 agcacctttt cttatagctc taccatcagc taagttaata ccactcattc tagtttgtgc
    17581 agaaaattct acaaactcag cttcctctgc ctcttttgtc ttacctgttg aacctaattt
    17641 catacctaaa tcaactatag attttccttc tggtgtatca tcttttaatg aacacataac
    17701 agaataattt attaaatctt ttgaatctgc atttccaact gctataaatt ctgatgcaag
    17761 tctattccca taagtaatcg tacctgtctt atccaaaatc atagtatcaa cgtctccaca
    17821 agcttctact gcttttcctg acattgcaat tacattaaat ctagttactc tatccatacc
    17881 tgcaatacca atagctgaca acaatccacc tatagtagtt ggtattaaac ataccaaaag
    17941 agctatcata gtagaaattg gaatctttac acctgcatag tttgccattg gataaagaga
    18001 tattaaaaca accataaata taagtgttaa ccctactaat aaagtattta aagcaatctc
    18061 atttggcgtt ttttgtcttg aagcaccttc tacaagagct atcattttat ctaagaacga
    18121 ttctcctggt tttgttgtta ttcttatctt taaccagtca cttacaactg tagtaccacc
    18181 tgtaacagat gcgaaatctc cacctgattc tctcatgaca ggtgcagatt ctccagttat
    18241 tgctgactca tccacagaag ctataccctc tataacttct ccatcatttg gaataacttc
    18301 tccagcattt actaaaacaa tatcaccaag ttttagctca cttgcactta tcattttttc
    18361 atttcctaac gtatctataa cccttgctac tgtatctttt ctagttttct taagtgtatc
    18421 tgcttgagct ttacctcttc cttcagctac agcttcagca aaatttgcaa aaaggactgt
    18481 tataaataag attattgtta caatcccatt ataagctctt aagttattgc cttcatcacc
    18541 aaacaaacta ggtactattg taagtatcaa tgttactata aatccaattt ctacaacaaa
    18601 cataactgga tttttcatca tgtatttagg atttaatttt ttaaaagctt ctataatgga
    18661 agttttgaaa atatcttttg taataaactt tgttttttta gtttccataa aactttactc
    18721 cctcccaatt ttttagtgcc acaacactag atgctccgaa ataggtccaa gcgctaaggc
    18781 tgggaaaaat gttaaagcac caacaattaa aacaacaagt actaaaacta ttgtaaatgt
    18841 aaaattatcc gttctaagtg ttcctatact ttcatttaca gcttttttag atgctaacaa
    18901 acttgatatt gctagtagta ctataataga cacatatctt ccaaagaaca taactacacc
    18961 tgttgttata ttccaaaaca tagtgttatc tcctagacct tcaaatcctg aaccattatt
    19021 ggctgctgaa gatgcaaact catacaatac ctgtgataat ccatggaatc caggatttga
    19081 aattccttca agccctgctg gtattgctac tgaaagacca gagaacatta gtattaaaag
    19141 tggatgtaat ataataagta aagcaataag cttcatttct tttccttcga ttttcttagt
    19201 taaaaactct ggtgttcttc ctaccattaa tccacataag aatactgcta ttatagcgta
    19261 catcaacata ttcatcagac ctacgccttt accaccaaat actacattta gcatcatatt
    19321 taaaagtggt actaatcctc ctaaaggagt cagtgtatca tgcatattat taactgtacc
    19381 tgtcgtaaag gaagtagtca ctgttgtgaa taatgatgat tgtgctatac caaatctaac
    19441 ttccttacct tccatacttc ccatactttg attaagccct aattgtgata aaataggatt
    19501 tcctgcactt tctgctttaa agcacactac tagacctatt aaaaatatga ttgacattgc
    19561 gccaaacaca acccaacctt gttttttatt ctttatcatg tgaccaaatg caacaacaca
    19621 agctcctggt aatatcatca tagataatat ttctactata tttgatatta ttgttggatt
    19681 ttcaaatggg tgtgatgaat ttgaaccaaa gaatccacca ccatttgttc ctaaatgttt
    19741 tattgattca agagctgcca caggacctct agctatatct tgaagctttc cttctatagt
    19801 agtcactgtt tctgtcccag caaaagtttg tggtactcct tgtgtaacta atataagccc
    19861 aataattaaa gctcctggta aaagtactct tgttgttatc ctaattaaat ctacataaaa
    19921 atttcccaat gttttctttt taccgactag acctcttaca aatgccatag cagccgcata
    19981 tcctgttgca gctgatgtaa acatcatata tattatcact gtcatctgac taaagtatga
    20041 aagtcctgat tcacctgaat aatgttgaag atttgtattt gtcataaaac ttattattgt
    20101 attaaatgat agtccttgtt ccatactttt aattccactt ggattaaaaa tatgcatact
    20161 ttgagttcta agtatgatat atcctataaa taccataact gcatttgtaa ataataaagc
    20221 taatgcatac tgtttccagt tcatttcttt tttcttatct atactacata ccttatatat
    20281 aaaattgtcc actttatcaa atagtctatc tccaaatgtt ttttgattag tagctatatg
    20341 atataaatac ttgcccatag gtattactaa aatcataaaa atcgctaaca ctattactat
    20401 ctgcaccata aaaattgttc cccccaattc aacttactta ttagccttat actgtttaaa
    20461 atttttcagg attaataagc gcatatacca aatatattat taacattata attacaactg
    20521 ccagaaacca cataggtaat ccctccatac attacattat tttttttcag tttgtttccc
    20581 acaccaatca gcaagtaact tcatggaaac aaatcctatg aataacaatg ctatcataac
    20641 tatatccaac attttagctc ctccatttaa tttaatctct tttacaaatt ttagttttgt
    20701 attgctactt catttatctt atcattttag gtataaagat aggattaaga aacatccatt
    20761 aatgattagt atttcataaa tataaaattg ggtttttaaa atgcacatct agcaatcgta
    20821 ttcccagtca ttccaacata attctatatt tgagatttgc aaaaacctag atgggattat
    20881 actttctata aagatttaga cacaaaaaag gtggcaaatc tgccacccct aataaaatct
    20941 aaagttattt aatttatatt aacattctgg aactatatca ttacttatat ccatatccaa
    21001 acttttacca tgataataaa taaaatcaat taccttgcca ctaattgtaa cagcaatcaa
    21061 agaataaaag atatcaaacg ctgatagata aaataatgat aacaacaata caataacatc
    21121 tgttgctaaa taaacttgcc ctatgttcaa agatgtagtc ttagaaatta ctaatgctaa
    21181 agcatcgtct cctcctgatg cacaacctgc ttttactact atacctacac cagttccaac
    21241 agttacacct gccaaaatag ttgataaaag catattttgt gattgtggta ctagaggtcc
    21301 tatagcttca aataagtcat ataacactga aaaagttata gaagctacta ttgaatatat
    21361 aaggaatttt ttaccgaaaa atttatatcc tactaaaagt agtaaaccat ctattacaac
    21421 atttgctatt gctggttgta tattgaataa attttttaac agtagtaaaa tacctaacac
    21481 tccaccttct gttatgttgt ttaaataata aaaattataa actccaaatg ctaatattgc
    21541 tgaacctaaa aatataagtc caatcctttc taaaaataac cgcctggcca tacatatccc
    21601 ttcctttttc tgaatttata tatattatat acaaaagaat tggctttgta taccgcactt
    21661 ttcaaatctt taattataca aaatttctat atttttttca acaatttttt tatgtttttt
    21721 tatgtttttt taatactaca cctacactat agtaattcca atactttaga cgttttcctt
    21781 ttttccataa gtttatatta gttttactta taatttagta taattaagtc tttgtttata
    21841 ttaatttttt atttgtttag aaatactatt caataattta tcactaaatt aaattaaaaa
    21901 aagagttact ttatatagtt tctaaagtaa ctctaaaaat cttatttatt atctatgaca
    21961 atttaccatt cacccttgaa ctctgaaata ttatctttgt taacttcaac ttgtttaatt
    22021 tgtgtaacag gttctatttt ttcaccttta aggaatttag atgccttttc tatgactaat
    22081 ccaccttctt ctacagcaga catataagtt gtaccatcta ttaacccatc agttatagct
    22141 tttaatccat ctttacttcc acccaaacct atgactttaa ctttatcctt aagaccagca
    22201 tcttttaaag cttgacctgc acctgtagca gaattatcat cttgacatat tataacatta
    22261 aaatcagttt tcttttgaat taaatcttct gttactttta aagcttgctc tttctcccaa
    22321 tttgaagttt gattataaac tatttctata ttactatctt ttaattcttt ttcaagacct
    22381 ttttttctta atacttgtgg agtagttcct ggtactcctt ctattaatac agctttccca
    22441 ccagtttctc ctaataagtt tttagcaatt tttccagtta atgctcctgt ttcttcctca
    22501 ttttgtccta tatatgtaac taaaccttta aattctccag ttgggttact tcctatagaa
    22561 tttgtaaatg ctactatagg aacgtctgaa tcattagcag ctttaactat tctctcactt
    22621 acagcagaat caactccaca taatgcaatc atgtctaccc ctttagccat aaaatcttca
    22681 gcttgtgata attgtttact agcatcccat tgagcatctg catattcaac gcttacacct
    22741 aattctttag attttgcatc tataccttgt ttagttgcta caaagtaacc tgttgaacct
    22801 ggtagtaata ctgctatttt cttttctcca tttttttctt tgtcaccacc atttgaacaa
    22861 ccaactaaac caaatatcat aacaagtgat agcaacaatg ctaaatgttt cattaatttt
    22921 ttcataaaaa aatccccctt attttttatc ttgaagcaag ttttttcttg ctataagaat
    22981 caaatccaac tgctgctaaa attattaatc ctttaaatat gtactggaaa tatacatcta
    23041 ttccaagaag attaaaactg ttgtttataa cactcataag taatactcct ataacagttt
    23101 taagtacaga accagaacct cctgccactg atgtacctcc tattactaca gatgcaattg
    23161 catccatatc atatccttca cctgcaatcg cagttgcagt cccaagtctt cctgttagta
    23221 cgattccagc tattcctgca agtacagcat taataataaa cactttcact ttgtgagaat
    23281 ctacatttat accattaagt ttacttgctt cttgattccc tcctacagaa taaacatatc
    23341 taccaaactt agttttatta agtacaataa acgaagctaa caccactata aagaaaatta
    23401 ctactggtat tggtatatta aacaaatatc catttccaat gtaatcaagt attggtatat
    23461 tttcagtaat tggatatcct cctgtaagca tcaacagcaa tcctcttaca atagtgacca
    23521 tagccatagt ggctattaat gatggtacat ttatctttgc aactactata ccattgatta
    23581 atcctattac tgcacaaatc gctacaccta agaacatgga tgcaaacaaa ttagttccac
    23641 cagtcgcaaa tttcattgtt atagcacctg caagagctgc tactgctcca actgatatat
    23701 caaagtttcc agatattata acaaaagtca tacctgctga aattattcca attatagaag
    23761 attgtcgtaa aagattaagt atattttgtt tacctaaaaa tgatggagtt gcaatactta
    23821 tagctataca taaagctaaa aaacctaata atactccata atctcttaaa tttaacttct
    23881 caaaagcttt cttcatctca gtcctccatt ttcatttttt atacattaaa tatactgttc
    23941 attatatctt gaacctgtat attctcattt tttaaatcat ctacaataga accttctcta
    24001 gttactaaaa ttctatcact tactcttatt acttcttcta aatcagaaga tataaatatt
    24061 actcctattc ctttacttga aaattctttg attgtattga aaatatcttc ttttgcagca
    24121 atatctattc cctttgtcgg ctcatcatat attattaaat ctaaatcttt ctctaaccac
    24181 ttagaaacta ctactttttg ttgatttcca ccactaagct cttttacttc ttgctttaca
    24241 tcactaactt ttattcctaa ttccttaaca ccttttttag aaaactcaat ttctttattt
    24301 ctatctataa acaaaaattt cttgaattta tcaatttgta ctgatgatga gtttgtgtat
    24361 atagcttgtt ctaaaattaa tcctagattt tttctatctt caggaatcaa tcctattttg
    24421 ttttttatag caaccttagg agaagttatg tctacttttt taccattgat aaaaatatct
    24481 ccactatctt tattatctat accaaatatc aaatttataa tttcagttct tcttgaacct
    24541 acaagacctg caagacctac aacttcgcct ctataaactt taaatgaaac attttttatt
    24601 ttgttagacc agcttatatt ctttacttct agtactggtg gctcttcata ttttgcaaat
    24661 gtataatcct tcttctcaat aagtttgtga ttatcatttc ctgtcataag ttgagttatc
    24721 tttaatttag ttaaatcctc tacatcataa gttccaatca taattccatt tttcattata
    24781 cttgcactat cacatacttt aaaaacttcc tctaatatgt gagaaatata gactattgtt
    24841 ttacctttag actttagtct acttattatt ttaaatagcc catctttttc attattagta
    24901 agtgatgttg taggttcatc cattatgata atttcagaat tacatgaaac agctttcatt
    24961 atctcaacca tttgttggtt tgctacactt aaattagaaa ctactatatg tgggtctata
    25021 ttaaaatcaa aatcctcaca aattttcata tattcctcaa gcatgtcttt cttgtttaaa
    25081 aacacacttc cttttaaaat ttctctacca agaaaaatat tttcaagtac tgtaagcgtt
    25141 ggtatcaagc ttaattcttg ataaattata ctaataccat tttctttaga atgatgtgga
    25201 tttttaaaca ttatttcatt accagtcaaa attactttac ctttagtttg agaatgaact
    25261 cctcctaaaa ttttaataag tgtagatttt cctgctccat tagctcccaa aagagctctt
    25321 acttctccct tttctaactc aaaatttatc ccttttaaaa catctacctt tccaaaactt
    25381 ttatatattt gttgtatttc caatatctta ctcactttat cacctcacta attccgaaac
    25441 cattttataa tttcatcaat attttctttt ggtgctgaca acaaatccca agataaaaca
    25501 tatcctttta ctgttgtctt atttatattt gaaatatttt cacttatgta cttaggtgta
    25561 acaggagata taatttcctt tttatttatt tcaactccta catatatatc tttattaaga
    25621 tttcttgtca taaaatcgat ttcctctagt acaaattcta taggatatac atctccttca
    25681 tactccttaa ttcctaaaat atctaagaaa tttctttttc caatctcttt attcttatgt
    25741 atggattcat caataaacct ttcaaattca aatggtaaac cagctggagc ttttgtagtt
    25801 ctatacatca ttggttttat aaaatctgct acttcttgta atttttctaa atcttgacct
    25861 gtaaagtaag aaataaaagg agaaaaagca tctataccta ttttcaatcc cttactttta
    25921 aaataactac ttatctctaa taatgccttg tatatatttt gattttttgt ttcaaaaaaa
    25981 tcttcccata ttttattttt aaatttgtat tttccattaa tgtattctgt tataccaaat
    26041 ggagtatctt cattatctat aactccctta gacacattat tcatttctct cattaacgac
    26101 tctttatcaa tattattttt tttatatata tttaaacaat ctttacaaaa acaattaaaa
    26161 acaccattta gtttattcga aaaagaacca taccttattt tatctaaaaa aattccatca
    26221 aatttaatat cactaaaata agtactatat attttcttta tattttttat attttcctta
    26281 ttatttgggc aataaaactc aaaattttca tcctcttgta aagcatagcc cttgacttcc
    26341 ttccccatat aatcaattag taaattactt ttttcaagta atcctatttc agaaaaacat
    26401 ggcaaccaaa gatatagttc aacattatat ttatttaata gttttttagt ttctacatat
    26461 aattcttcat ctacactcca tcccatgata accttttcta tatcaataca gtccaataat
    26521 tctagtagtt tacattttat atcattaaac tttatttctt tagccttaaa acctccagta
    26581 aaaatttgta ctattacctt ttttctatcc atgcttgacc ctcctattag ctaatgccat
    26641 agcaagtctt atccccccat atactggctg attttcttga gatataaatt ttaaattttt
    26701 atctgtattt tctgttttta aattattcat taacatttga gcatatacag ttttatttaa
    26761 tgtaccacca gacaaaacta tatttatttc gcttttatcc attttcattg gattagatat
    26821 ttttctcaat attacttgag ctaatttaga tagttcagtt gatgattctc ttaatatttc
    26881 aatagccaat ttatcacctt gttcggcaca atcaatcact aatgaagcta agtttgctat
    26941 ttcaacatta tcatttatat cagtaatcat atatgcaagt tgttcaaaac tatttgctcc
    27001 aaagaagtcc aagacacaac taaataaatc actatactca tgacattcat cacaataaag
    27061 caatactttc tttaataatt ttctccctat atcatatcca gaacccaaat cactaaaatt
    27121 atacccccat ccaccaactc tatatatttc tccatcttct ttaattccca gtataattga
    27181 acctgtccca gccacaatta ctaagccagg ggaatcagct tgtgcataaa aagctaaaac
    27241 tgcatcattt gccagatata ttttttcttt gttaatccca atttttaata tttcatccat
    27301 tataatctta taatcattag gagaatcaca accagatata ccaaatacag tgtactcaat
    27361 atcgtcataa tcgatatata aatcttttaa tattttttta aatccattat taatattttc
    27421 atatgttttt ttaattccaa ctgacttata attagttgaa ccagtggtat gttctttaag
    27481 tatgtttcca tctaagtcac ttacacaaaa tttagttttt gttccccctc catctattga
    27541 aaccaaatat ttcacattaa tatcctcctt ttttattaga taaggaatat tgatagtcag
    27601 tttcttttaa acacatacta attactccat ttatacttcc ttctctcaat gagtctactt
    27661 ttattaattt aggaaaattt tcttctccaa cattattgct tacatctttt tctataaact
    27721 ccatgtcact cttggttaat atactacact gtataatcat agcttgtgga tttaggacac
    27781 atacaagagt ccttataaag tttgatatta tagataaaat ttctttctta aatttattat
    27841 ctttactttc taaatatctt tttcttaaac tcataatata atcttcaaga tttaagttgt
    27901 tagagttata cttaccaaat tctatattta gataacttaa ttctccagca aaatttgtag
    27961 aacctaaata caattcacca tttataacta tacctgaacc aataccatct tctaaataca
    28021 taagagccat atttttatat ttgtctttat actgtttttg ataaatccca atagtagtaa
    28081 tatttgtatc attttctata aacactttag tattgtattt actctttatt aaattttcta
    28141 aatttatacc ttcccaactt tttatagttg gtatattttt aactacacct ttttttacaa
    28201 ctcctgggac accaattcca atagccctta tattgtttcc tttttccttt tttattagag
    28261 tatctataac aaaaaaagta tcttggttaa cgttttcata cgtatccatt cttatattaa
    28321 attcttcttg ataaattatt tcacctaata aattggcaac tacccctata caacaatttc
    28381 tataataata taaagctatt atgtgttcta agtttgcatt tatttcaaat aattgagctc
    28441 ttcttcctcc agtagattca cttagtccac ttactttaac ttcatttttc aaagctaatt
    28501 gctctacaat cttattaact gtagctaaac tcaattcagt aatgcttgaa atttctggtt
    28561 ttgttatagg accttcattt tttataatgt tttttattaa atcttcattt acttttttta
    28621 tcgtttgcgg ttttccaact atatttttca tagacaacac cttactttat aaactttatt
    28681 tattaagttt agtataatat ttacaagtta aatttgtcaa taaattttca ataaaacttt
    28741 aaaatcaaat ttctctattg tctgcttcat aattattctt cctgactaag ttttattgtt
    28801 catattcctt tcaactgttc atattagttt gatgatataa atacatatta tagtattgac
    28861 tataataaaa tatcctcata caaagacagt tgcaattatc tatctgaata tatataataa
    28921 aaatgtaaat ttgtcattga actctatctt aaaagcgaaa tcaatgttca gatgaatttt
    28981 tcaaaccaga aaggagtatt tagtttatgg gaattggaac ttgtgcatag caaaggctat
    29041 tgtacataat aaaaaatttt atttttaatt gtgagtggca gtagcctttg ctaatcctaa
    29101 agaataatta tttaggaggt gcaaaatgag ctatcaaaat tcgattgact tactgcctaa
    29161 agaactgatt gagcaagtgc aagaatatat agatggtaaa gtgatttata ttcccaaaaa
    29221 gcaagagcac aaaaagcatt ggggtgaaaa cacaaacacc aaacaagtct tagcttctcg
    29281 taattctcaa atctgtataa atttccaaaa tggaatgagt atcaagcagt tatcagagaa
    29341 atactttttg acagagaaaa gcatacaaag aatacttaaa cagcaaaatt tataattaga
    29401 aatgtgtcac gttttatgcg tggcacattt ttattctaag aaagaacatg aggtttttca
    29461 ttcataccta tattgttata attaacttaa caaataaaaa ggaggaaaat atatgtcata
    29521 ttttcagtat caaggtaatt cttgttttta taaagaatat ggacaaggta aaccgataat
    29581 tttcctgcat ggtaatactg gttcttctaa tatgtttaaa gcacttgtgc cattatatgc
    29641 agaaaatttt agatgtatct taattgattt tttaggtaat ggacagtcgg atagagtttc
    29701 tcaattttct cctgacatat ggcatgatga agccttacag acaatagcac tgattgaaca
    29761 tttgaattgt ggtaaagttg gcttaattgg aacaagtgga ggagcatggg cagctgttaa
    29821 tactggatta gaacgtcccg atttagttga agcaattata gcagatagct ttgatggacg
    29881 tactcttaat gataacttca tagataatct aatcactgga agggaaaaat caaagcaaga
    29941 tattcaagca cgaaaattct atgaatggtg tcatggaaaa gattgggaga atgttgttaa
    30001 tcttgataca aaagctctat tgcaatgtgc aaacgagaaa cgccctttgt ttcataaaga
    30061 attgtgtaaa ttagaaatgc caattttatt tacaggaagt aaggaagacg aaatgtgtcg
    30121 tcataatctt gaagaagagt acaagcaaat ggcaactttt atttcaaaag caagtattta
    30181 catttttagt cagggaggac accctgctat attgacaaat gcagatgaat ttgcacagtt
    30241 agcaaaagtt tttttcaagt attaactaaa aatattaact atttcataaa cattatttag
    30301 aaaagaaata ttaagttagt atttaaacac acagatagta ttattctatt atatacagat
    30361 ataaaagaag cgccgagacc cacaaaatat agtatttatt caggttctca gcgttttcct
    30421 tatctcagtc taatactaag tctcgactgc caaaataaaa ttttttacta ctttttaaac
    30481 ctaacctcag cagcctttgc atgtgcatat aatccctctt cttttgccag cttttcagct
    30541 acaggtgcta atgattgtat agctttttca ttaaatatct gctttgtaca agttttgata
    30601 aaagttccaa cccaaacacc accagtatat cttgatgctt taagtgttgg taatgtatga
    30661 ttggtacctg aaacatagtc accaaacacc tcagcagaaa gatttccaat aaataaagaa
    30721 ccatagttag ttaatctatc acaaatatca ttatactcat ttactgcaac ttctaaatgt
    30781 tctggtgcat aaaaattact tattttaatg gcttcttcca tatcatcaac caatattatc
    30841 tctccattat tttcccatga actatatgct atgtcctttg ttggcaaatc ttttagcata
    30901 ctttctatat ttttttcaac tttttgtgct acttccaagc tatttgtcaa taatattcct
    30961 cttgcattta aatcatgctc acattgtgct aataaatcag ctgcaatata aactggattt
    31021 gaagtttcat ccgctattat aagaacttca cttggcccag cgacaaaatc aatccctact
    31081 tgtccataac attgtctttt agcttcagtt acaaacttat ttcctggtcc tacaattatg
    31141 tcaactggat taatcttttc tgttccatat gtaaatgctg ctatagcttg aactcctcca
    31201 gttgcatata tttcatcagc acctgctata tccatagcta ccaatgtctt aggatttatc
    31261 ttttcagtat ttttcatagt tggtgaacat gccactattc tttttactcc tgctactttt
    31321 gcaggtatta taagcataag agctgttgaa aacaatggat actgacctcc tggaacatat
    31381 gcaagacatg actctattgg aatattagtt tgacctaaga ttacacctgg ataaatttct
    31441 ttttcaaaag ggttttcaaa acttagtttt tgagctttag caaattctct tatatttttg
    31501 gcagcaatct ttaagttatt tataaattcg tcatctacat gcttataagc ttcctttatt
    31561 tcttccttag taattctaaa ttcatctctt gtatttctat caaacttctc attatatttt
    31621 ttaagtgcaa catccttatt atttcttata tcttctatta tagaacttac ttgctctctt
    31681 aatatctttt catctacctt ttctttcata cttgatgttt taatactttt catttttacc
    31741 tcctaatttt acaatctcat actcatttat tttacaatat tatcctcatt taagatatat
    31801 aaataaataa aaactcccac cctctatcaa aagctagagg atgggagttt aatcatattg
    31861 tactcacctc actaactgag cactatttgt ctaataaata tacttatatt tcttctaatc
    31921 attatttttt tctgaatttt ctctttatta tacataatat gatatatttt aactattgat
    31981 ttgtcaatta atttttttat tttctacagc agttacaatt acatttatgg catttatggc
    32041 aacaattatt attgtttcct ccattatttc catttcttct agcttcacat tccatacctt
    32101 catcaaataa tctagcagct tctgctaaat attcgattcc tttatagttg tattttgaat
    32161 attcatccca gtatttttct gcttcttccc ataattttct agcttcacat tcatatgcta
    32221 atgcctcttt ctttttctct gaagattcac agaatgcttc tcttgcttct ctttctctta
    32281 tctcagcttc tcttctaaat ctttcacagt ttctaggtct attttctcct gaatctgaac
    32341 atccacagtt tgaacctctt ctttctctat cttcatcata tgcttctctt tcatattctt
    32401 ctctatcatt ttggaaccat ttagctgtag attctcctct ttcataccaa tcttctgaat
    32461 aacatttttt attttccata taacatatct ctccctttaa ttttaatttt tttatgagat
    32521 gttttgtttc atctcatact atatagtatt aaaatcgtca aaatatgtta catataaact
    32581 tatatttttt cttttttaac ttattttcaa cattttattc attttttcgc agtatccttt
    32641 atcttttatt agacataaac tgttgtttat ttcatattca gaatcagcat aaaccataat
    32701 agcaagttca tcatcatcat ttaaaatatc gaattttact aaacaattct caactatatt
    32761 tagaatttca tctttattac attttttaaa tctaagaagt gctccacact tttctctaac
    32821 tattttatta acttgtattt tatctttagt aaaagaatct atacttgaaa ctctattatc
    32881 tattatatct ataacatctg aataaaccgc acttcctgtt gctaaacttc cagctccttt
    32941 acctacaaaa gaaacttctc ctactgaatc tccattcact atgacagcat tgaactcatt
    33001 gtctattcta gcaaggatat tatctttttc tactaaaaca ggtctaacaa aagcacttac
    33061 tttatcattt tcatacttac tttgaccaat caattttatt ttacaattta atttttttgc
    33121 atattcaata tctttttcat ctatatctgt aattccttct aaatacacat ctttccaata
    33181 tactctattg tcataagcta gagttgataa tattgatagt tttctagcag catcataccc
    33241 taatacatct gactctggat ttgcttcagc aaaacctaat tcttgtgctt gtcttaatgc
    33301 catatcataa gatagattct catcatacat tttagataat ataaaattag tagttccatt
    33361 taaaattgca tttatactat ctatattatt tccttctagt gattctatta ttggttttaa
    33421 tactggtata cctcctgcaa ctgaagcttc aaatttaata cttactttat tttcacttgc
    33481 taattttgca agctcatctc cacattcagc taacaaatct ttatttgctg taacaacatg
    33541 aattttattg tttaatgctt tttttatata agataatgat ggttctaaac cacccatgac
    33601 ttcaacaagt atatctatat ctttattaaa tatatcatct atattattag ttattatatt
    33661 agaatatttt ttatccttat gtttttccaa attattaact aaaattccaa ctatttctat
    33721 atttcttttt tctttattat tatctattat atctataagt ccacttccta caactccata
    33781 tcctagtact ccaattttca ctttattctc cattttattt tcctcctaaa tttataattt
    33841 tccgattttt ttttattttc aatcttttat tgcaataaaa atagactcat ccctatataa
    33901 agggacgagt ctatactcgc ggttccaccc aatttgattt tatcatttat tgataaaatc
    33961 cactttacac tttaacgcag ttaacggtta gtcttactaa aactttttaa agtaacttca
    34021 gaccacagct cctaggtagt tttcaatttc tcaaacttag aaagtctttc agcctgtgaa
    34081 ctttcctctc tgatagtcga attaaattta ctcttcctat tcattgcttt tatattttct
    34141 ataattatta tgattactgt ttatattttt aaataacaaa aaactctcat ccttataaca
    34201 taaggacgag agtattattc ccgcggttcc acctaattta gttttcatat gtcatgaaaa
    34261 ctcactttac actttaacgc agctaacgac tattcttact aaaatctact aatgcagatg
    34321 acttcagaaa gtagctccta ggtagttttc aatccctcta atttaaaaag tcttccagcc
    34381 aacggactct tctctctggt aatcgaatta gatttactct tcctattcat cgccttttta
    34441 tttattatgt tgtaatttat tttaaaccct tctaaatcaa ttgtcaatac ttttttaaaa
    34501 ttttattttt tatatttaaa cttataaatc cgagaactct atttctaatt ttttacttaa
    34561 ttcattgctg atttctcttt tatgattata tttttcaata ttatttttac ttctaacagg
    34621 caatgaaact ataaattcac ttccaatacc aagttgactt tttaaagata tacttccctc
    34681 atgcaactca accaaagatt tcactaatga aagtccaata ccacttcctt taactctgta
    34741 agaaagttca ttgtcaatct gttcaaacct attaaatatt ttatctattt tttcttttgg
    34801 tattcctatt ccactatcag atattttcat attaaataca ttatctttca catataaaaa
    34861 tatatttatt tttccatctt tcttattgta cttaattcca ttagaaagta aatttaatat
    34921 tattctttcc aatttttctg aatcaaataa cattgaaaat tcttctattt ctgtatcaaa
    34981 tgtaagttgt atattatttt ggcttgcaaa atcagctaca gacatacata tctcctctat
    35041 aaatgcaatt atatcagatt cttctggttt aaaatctaaa tatcctgatt gcatttttgt
    35101 aaaatctata acattattta ctaatcttaa tattctattt ccattttgtt ttattatacc
    35161 taaatttctt tttaaagtat catttgattc tattaaatct gcttcttttt tttctatcac
    35221 ttgtaatgaa gatgatatta agtttatagg cgttctaagt tcatgggata aatttgcaaa
    35281 aaattccatc ctagttttat ccatttcctc ttctaatctt tttcttctac ttatatccct
    35341 tgctgttcca attattccaa taacacttcc atagctatca aaaaacggcc tttttgtaat
    35401 ctcaatacac ttgttttcat cattcatact tatatttaac tcaaatgtaa ctactttttt
    35461 attttcaact acacccttat cactttcaaa tatcttatct attaaagctt cttcttctga
    35521 tattttataa ctattcttcc ctaataattc ttctttattc atacaagcta aattcaaaaa
    35581 acctgaatta aatccaatat aatttccata cacatcttta taaaacatat tctctggaat
    35641 tgagtgttct atcatcttca ttaaatcttt tgagttataa tttctattac tgtaaccact
    35701 atatattata ttgtctacta tccctatagt acctaaaagt tcgtcttcat catacaaaaa
    35761 atatttttca accataaaaa atgtttcttc atattcaaaa agattttgta aaaatctttt
    35821 tttactagat actccttctc tataatcatc tctaaataat tttcctgtat tctctcccca
    35881 tacttctata tcatttttac caaagatatc tttcgttgtt ttaatgttgt tatttaatat
    35941 tggtattaaa ttttcactgc aatctatata tgtgccttct tcgtctttaa tataagctac
    36001 tttttccttt ttatcaaagg aatcgattat ctgtctataa tcaaatacaa ttttaccaaa
    36061 atacaaaatt atgtatgaat cccctattat ctgttttttt atatttatcc aatttttaag
    36121 tgtcggaatg taccttttta tacaatccac ttcatttgag aacaactctt tgaaatattt
    36181 tttagactta aatacaggca atacatctag tatattttta tcacaaatac cttcttctaa
    36241 ttgtaatact ttgcccatac ccttacttat atattctact cgatattcta tatcttcttg
    36301 tctctttaca attctacaat ataaaaaagg tacaggtata ttttttataa aatttacata
    36361 tttattatgg gtatccatat tctaaccact acctttcaat gttatttata ataatactac
    36421 ttaattaatt atatgacaat tttctattaa ttataacata ttttataaga tatacttgtg
    36481 aataaattta attttatgta atttattatt tgtttataat tatttatgtc gcttttttct
    36541 aaatctaatt acagtagctt attctaatat attggtaatt agaaatacag gcgcatataa
    36601 aaacacatga ctaaatactg cattatttga aaatacagaa attaactcat taggactata
    36661 atttttattt agtatatttt ttgatactta ccaattttat ctcttactta tcaatagtca
    36721 tctttttaaa gatgattata aattaaaact atcccattaa ataaaatttg tcaagttata
    36781 ctaaaaataa aaaaagccca gaaaaattct agacttttta acttaaatgt caatatttct
    36841 ttattaattc aattacattt ataatactaa atactctctt ttattttaaa tactttactt
    36901 taacatttca tgtcttatct ttctaaaata caatcttgaa tttacctcat agccaataac
    36961 gatagaccaa ctactaaaat acatccaggt cataagtaca attatttcag ctatacttcc
    37021 atagattact tcatagttag catagtaatt tgtataataa gaatataaaa atgatactaa
    37081 aaaccaagca aaagtagcaa aaatagcacc tggcgcagat tctttaatag ttaacttttt
    37141 atttggagtg tatttatata aaagtgtaaa tatcactatt attgttatta tacctacagt
    37201 gtatcttaga atatcccata tttttataaa tatctcatcc aatcctacaa gattaaaaat
    37261 aaaataacct attttttccc cataaactaa aaatatcata gataaaaaaa taaggaccaa
    37321 aagcattatt gtaaataaaa atgatataga taaaatttta aaaaaagacc tagtttcttt
    37381 aactttatag gctctattca ttcctttaat aagagctttt actgctcttg aaaatgtcca
    37441 catagtaagt acaaaactga ctgctaaaaa tttcaggttt ctattgtcta ttgcagaaac
    37501 tatgattgaa gataacacag caaaagcacc ttctggcatc atatttctaa acaaagctat
    37561 atacttattt aaatgaagaa tcggtatata agctatagaa cttattgtaa atattaaaaa
    37621 aggaaatatg gacaacatta agaagaaaga tacttctgct gactttgaac ttaattcact
    37681 gtaattggat tttgcaatta tatattcaat caatttctta tctataccaa acataaatat
    37741 ccccttttct atcaaattaa tttcattata caatattaat tatactcaga ttcattctat
    37801 aatataatta tttaagtaaa aataacacaa ataaaagctg aaaataacaa ttctactaac
    37861 atttccatat tctactcaat gaaagtaaat tataattttc agctaaataa atattattac
    37921 aaaattatct tataaaataa taatatttta ttttaatttt tcgaccatgt catctatata
    37981 ctttttcaaa ccttgaatct ttttatctgc ttcaatatca ctagctcctt ttacagctat
    38041 gtaaaattta agttttggtt ctgttccaga tggtcttata gctacccaag attcatcttc
    38101 caaaaagtat tttaaaacat cagatttagg aagattatct ataccatttt tataatcttt
    38161 tttatcaact actttaatac tatttatata atcaatttga ttttctctaa aataagatat
    38221 tatttctttt atcttttcta caccttctat accttttaaa gtcaaagata tagtctgttc
    38281 tttgaaaaat ccatactttt tataaagttc tattaagcca tcatataaac tcataccttt
    38341 tgaatagtaa aatgctgcca tttcagatat taacaatgaa gaaactacac catctttatc
    38401 tctagcatga gtcccaacta agtaaccata actttcttca tatccaaata cataacttct
    38461 atttttattt tgttcaaata attttatttt ttcaccaata aatttaaaac ctgtaagaac
    38521 atttaataca tcaacattat ttgctttagc tatatctgct ccaaattctg atgtaactat
    38581 ggtttttatt attgtaggat tttcttttaa tttattattt tcttttaatc cttctattat
    38641 ataatgtgtc agcataccac caatttgatt tcctgtaagt aaagcatact cacctgtagt
    38701 agtttttact gctacaccta ctctatcaca atctgggtca gttgctatta caaggtctgc
    38761 tcctttttct tttgcgagta atatacctct atttaaagct tttttctctt ctggatttgg
    38821 ataatcaagc cctgcaaaat ttgggtctgg attttcttct tctggaacta caattatatt
    38881 ttcaaaacca acttctttca atgctcttct tattggaaca ttccctgttc cacaaagagg
    38941 tgtataaact atcttaaagc ttttacctac atttttaact aaatcttttc ttataatttg
    39001 gctttttact gcttctataa atttagtatc ttgtgattcg tccaacaaaa ctaccaaatt
    39061 tttattagct tccgttaagg ttggtattgt actataatca tgtatactat ttactttctc
    39121 tgttattgca tttgcaatct ctggcataac ttgtgctcca tcttcccagt ataccttata
    39181 tccattatat tctggaggat tatggcttgc tgttatgact ataccagcga ttgcatttaa
    39241 gcttctaaca gcaaaagata attctggagt agttctcaaa ctatcaaata tatatgcttt
    39301 tataccacat gctgccaaag tatttgcaga ttctatacag aattgtctag acttgtgtct
    39361 attatcatga gctattacta ctcctctact tgtttcttct tttgttgtgt tttctaatat
    39421 atagtttgcc aatccaaaag tagctcttct aactgtgtac ttatttattc tattcgttcc
    39481 tgcttcaatt attcctctaa gacccgcagt cccaaattct aaatccttat agaatctatc
    39541 ctctatttct ttttcattat tttttatatt ctccaactct agctttgtat cttcatcaaa
    39601 atatgaaccc tttatccact cttcataagt tttagtataa tccataataa acccctctct
    39661 actgtatgta tttattgcaa ctgacgcata aacaagcatt ttaatgtaat ctataactct
    39721 tctatatact tattaattga attaataaaa atgtctgcca ttattctatg accctctttg
    39781 gttggatgta acccatcaaa atataatttt ttatatcctt ctagttccat atttttatta
    39841 tattctgaat aaaaatctac gaaattagta ttaaatgtca tacaaaattt ttttatccaa
    39901 taactatatg attctaagtt ttgagatact atattaaaat cagtaaaaca actccaatct
    39961 tccctaacat ttggaatatc tggttttaat ggtataccta ttataggtat aatatttttt
    40021 gaatatgact gatgcaccat agccattata ttagaattaa taatttcatt tcccgcacca
    40081 gcaataaaat cattggtgcc acccattata aaaactatat caggagagtt ttttacaaca
    40141 tcttcattaa acctagctaa cataccactt gttgtatcac catttatacc cttatttact
    40201 atttctaatc gagtctcttt atttacaatg tttgtccatg aattacttcg tgatactcca
    40261 aatccatacg ttaaactgtc tcctaagcag actattttca tcttatcccc ccttagatat
    40321 atttttatgt tttaagaata atctaacagt ttagttaatt catattttat acttttttgt
    40381 ccttcaagta aagttattct attcactaat ttaatgtaga tatatatcta tacttttcct
    40441 tccccaattt gaagcttttc cttcactgtt cataaaaatg tctattctat tacctttaat
    40501 tgcacctcca caatcttctg ctacaaaagt catacccagt ttaggtatat atacttttgt
    40561 tccatatggt attacacgtg ggtcaactgc aataacaccc catcttggag ttgtaccagt
    40621 agatgttatt gtatctcctg catatgcagt ggcaaccact ttcatatgtt tgcctcctct
    40681 tatatttgct cctcttgatg ctattagtac tttttcttta gttccatatt ttataatttt
    40741 tgtagttggc tccttagata ttacttcttt tgataagact tttttgacta acttgccatc
    40801 atggtaagtt aaattataga ctagtttttt ttctccttct tgaccttctt gataaacttt
    40861 agattttcct tttaacaaat tactatcatt tacatgcttt gtatcaaatg gaattttttc
    40921 aatctcttct tttttacttt tagttacctt aacaactttt attttcatat catcttttaa
    40981 ctctgtatct aaacttggat taatcttatc ttcagaatta tactttacac cctgttcatc
    41041 caaaagttcc tcaactgttt tcttaaagga agatactttt tgctcttgac ctttaacggt
    41101 taaacttatg tcttctttat ttaatataga atatatactt gccaaaccca ttaaaattga
    41161 tacacttaat aaagatgata ttattatcct cttttctctt ttctccataa taatctcctt
    41221 ccatgttcaa attttaaatt aatattattc attacagttt taaccttata ttatatatgt
    41281 atattatagc tttccaaatt tatgtaactt ttttgtaact tctaaaatta ttaagattac
    41341 ttttagacct agaaaagtcc ataaatatac tgtttttaga ttatcaaagc ttaatataat
    41401 agtttttagt tatgtcttaa gcaattatct cttagactat gagatataag cataaataaa
    41461 agaaagaaat gacactcaac ttaaaacaca ttgtatgcaa atactgacta tggtaaaatg
    41521 aactcataaa taaattggaa ttttcgtgat taggaggagt aaagtgaaat ttgaacggtt
    41581 aagaaccagt gaaaataaat tatattacaa ggctatggaa ttatataaaa ttagtttccc
    41641 atttcataaa caaagaaaat cttctttgca agcagaaatt ttgaaaaata aagaatatca
    41701 attcaattta atttatgata aaaaccagtt tgtcggcata attttatgtt gggaaacaga
    41761 taactttatc tatgtagaac atttctgcat ttttccagaa attcgcaata atagatatgg
    41821 gcaaagagcg ttagagcttt taaataagaa aggaaaaact attattttag aaattgacct
    41881 tccaattgat gagatttcaa tttatcgcaa ggcgttctat gaaagagtaa aatataagac
    41941 aaataattat gaacacattc atccaccata ccataaaaaa tttaatgggc ataacctgat
    42001 tgtaatgtct tacccagaaa agttatctaa aatggaatat gataaattta atcactatct
    42061 aagaaataat gtcatgagtt tctaaatata attcctattt attgaactgt ataaaacttc
    42121 ataattaaat aaaagagatg tctcaaaata taaataagac tcttttccat tgtttaaata
    42181 ataaagatat attggatttc atacaaagca ggatgcatca tgaactaaaa aattcatgat
    42241 acatcctctt tttcacgctt aaagttaaga aaacaggtga gattttatag acaaaattac
    42301 attttatata acaaatttta ttctattttt ataaatttag gtttctattt ttgtgtaata
    42361 atacagataa ttcagatttc tattttgaaa cctctatttt tctaaataac tgtttaagac
    42421 cgacaaaaaa ggtgattcaa atttttattt tgaaacactt tcttttaatt tgattaatct
    42481 ttaagtccta tattaaattt ttacttattc tttatttttc aacatttttt ccatatctaa
    42541 aatcttttca attacatcct taaaaattgg ttcttctcta agctcattat aactgtcatc
    42601 attaagtaca attaacaagt ttgtatatat attaaaagta tataaatttt tagactctac
    42661 cttatcaaaa aatctcattt gacttacatc caaactttca tgtataggat atttttctaa
    42721 tatttctttt agactatata aagctttttt tgcttcttct ttcatgttaa aaaataaaaa
    42781 aattagagat aattccatat aatccatata catactaaaa aatgcacctt tatcttcact
    42841 gagcattttt tttatttcta aagaaagatt tgcatacccc agcaacttat tttttatata
    42901 ctccatttct ctctcaattg catttttatt aaaatttcta ttctccaatt ttttctttaa
    42961 ttccttgtat tgatttttgt atacattaat tattccctta caatccatgc ttatattaaa
    43021 tacattacta aacaatttat tttgaagcaa tatttctgct tcttcaagtc tattttgctt
    43081 taaatatatc atagctaaat taacacttgt atcccctatt gacttatgta tcatatttaa
    43141 atacaactct gccttttcat aatcctccaa catacaatat gcgtttgata atataataag
    43201 agctgtttct tttatttgga tatcatctgt atttctcact atatcttcaa gaatattcgt
    43261 tgtttctttt actctgtcat taagttcact cttaccaaat ttatatgaat acatctgata
    43321 cagcgaacat atccttagct tcaataaata gcttttagga tacttctcta tatacatatt
    43381 ggaaagcttt tttacagatt ctaaatcttg tctaaattct tcttttggtt cttctgttga
    43441 taaatcaatt tcaattctag caaagcctga ttctaattct ttataaattt tcattacttc
    43501 ttcttcactt aaatcaatct tgtaatttaa aagcttatca atagacacat taaaaaagtc
    43561 tgctaataaa ggcaataatt ctatatctgg gtaagaattt ccactctccc acttagatac
    43621 tgctggtgtt gacacaccta taaactttgc taattgttct tgagttaaat ttcgttcttt
    43681 tctaagacac tgtatgactt tacctatttt aatttgcata tatacctccc tagtacatct
    43741 gttgtaattt aagtatataa aaaaaatatt tactagtaaa tcgagggaaa gttaacaaaa
    43801 tgtctgtaat ttaaccattg gttatatttt aatctaaaac cctatctcat tgtcttgaaa
    43861 tataaaatct attaattaag tttaagtata acctaattaa tagattcaca ttaaatagca
    43921 tttaaatatt tatattttct ctttactaca acttactata tttaaacttt caaggaattt
    43981 taaataaaat aaattccata tacaatgata ataatactga gtgttaataa tttatttata
    44041 ataacaccca cctattcaat aaatggcatt atagcaaaaa atcacttctg ggctctctct
    44101 tttctacttt gcatatacat aatccacttt tcaaatatta tatataaata aatttaaagt
    44161 aaatatatca ttatattttt ttattacaat atttctattt tattatttaa ccaacaaatt
    44221 atatcctcaa aaacttcttc tctatttatt tcattcaaca attcatgcct tccatctttg
    44281 taaagtttgc aagttacatc ttttactcct aattttatat atctatccct tagtcttaag
    44341 actcctttac cattttttcc tatggggtct ttatctccag atattatgta aataggtata
    44401 tcaagtggca ctttttttaa gttttcttta tcctcaattt cttttaaccc ttgaactaaa
    44461 tcataaaaaa agccacaact acacactact ccacaaaatg ggtcatttat atatttttca
    44521 acttgttctt tatctctact taaccagtca aacttagtct tttcatttct tcttattatt
    44581 tcgccgccaa atatcaaatt atttatttta ttacttttag aacgtcttcc atacttttta
    44641 atctcacgat tgattatcag atgagcaaga tttagaatga ttccttgctt tccatttgaa
    44701 ccacatagta taagaccaga taaattatta ctgtaatcca ttatatatct ttgacttgca
    44761 aatgaaccca tgctgtgtcc aaatagataa attggcagat cttcattttc tttctttata
    44821 atatttgtta aagtatacat gtcctctaca agacatctaa atccttcttt ttcagctaaa
    44881 tgtccaacat tttctattat ctttgcagtt ttcccatgac ctctatggtc attgatatat
    44941 actatataac catttttagt aagtaccttg gcaaaagtct catacctttg tgcagtttca
    45001 gccattccat gtgcaatttg gataactgcc tttggttttt ttatattttc atcttcccat
    45061 ttatatgtat atatatctaa accttcctct cctttgaaag taaagtttgt acacttcata
    45121 attatccctc cagattgatt ttattcagta tattaattta tatattatat tacaagtatt
    45181 ttcaagtcaa aatctttcta aaaaatttaa gaattattaa ttcctcatag aagttatcat
    45241 tggtacatag aagtttttac ataaaattat acaataattt aattaattaa tagttacata
    45301 aaaaagtaat actttaatat attttgacac catattgaca ttagaattaa ttcatgatat
    45361 tctgttttcg acaaaacaac atctaaaaat aaaggagata tatttcatgg attattcaaa
    45421 tgaactaaaa gaactctttt taatgaatca aacatatgca actcttttta cccttacaaa
    45481 caaaatacaa atagaaggag ataaatattt tggtatactt acttcaagac agtatatgac
    45541 tattttatca attcttcatt taccagagga agaaacaaca ttaaataata ttgcaagaaa
    45601 aatgggtaca agtaaacaaa atataaatag actagttgct aatcttgaaa aaaatggata
    45661 tgttgatgta attccaagtc ctcatgataa gagagcaatt aatgttaaag ttacagactt
    45721 aggaaaaaag gttatggtaa catgtagtag gactggaata aactttatgg cagatgtatt
    45781 tcacgaattt accaaagatg aattagagac cttatggagt ttactaaaaa aaatgtatcg
    45841 ttttaatggt gaagaacaag atggctttga agaagatgct aattttatgg aatatgaaga
    45901 aatagataaa attaaatctg aagcacttga agaatttgca aaaagaagaa agagagtaaa
    45961 taaaaacgat taatactatc gcatctataa tactactttt ttatagtaca atattttata
    46021 taactataat gtcattttta ttttttaaca aaacaatcat tacatagatt aggtgttctc
    46081 aggaactctt tttctgtttt ttgtacaaaa aaaggagatg attagacttt tattttaaag
    46141 catctccttt ttcactatta tgattattct actcctaaaa tcatatcttt taatttttgt
    46201 aaatcattaa ttggttgact acaactatta ctttgacaca cgtaataact aatcttatca
    46261 tccttcaatc tatagttatt taaaaaacct ataatagtgt tttcttcatt gtatttcttt
    46321 gctaaaaaag ttgcatttgg tataaaattt tcacttatca attctttaaa ggcaattaaa
    46381 tcagaatctt ccttaaaaat acatataatt tccttagtag aataaagttc aaacatcaat
    46441 gaaagcatat aaaagctgta gccagctgga gaactcttta cattatccac atacagtttt
    46501 aattgtttat atgacatttc ttcaagtcta ttatctccag taattttcgc aagtcttata
    46561 agattgtata attgaacaga atttcctgat ggaatagctc catcatacaa atcttttggc
    46621 cttgctatta aattttcact atctttacca tatatataaa atccactttt ttcataatcc
    46681 caaaaaaggt tgatacagct ttcattcaaa tttaaagcct tttctaaata tttcatatta
    46741 aatgttgact cataaagctc tatatacgcc cagattaaaa aagcataatc atctaaatat
    46801 gctaaatagt cggaacttcc atctctatat ctagctaata atctacctga ctcatttaca
    46861 agattattat ttataaagtt aagacactta tttgaatact ctaaatatat gtcatttttt
    46921 aaagtactat atgcctttgt taaagcaact atcatcaatg cattccatga tgtaagtatc
    46981 ttgtcatctt tatgcaaaga tgttctttct tttctatatt caaatacctt tttactaagg
    47041 tcagctatct tttcattatg tctctcatat tctttatttt ttataagatt aggaatactt
    47101 ttgccttcaa aatttccact actagttata tcaaaataat tattaaagaa aattccatct
    47161 tcttcaccca aaacttcaat tatctcaaga ggattaaatg tataaaattt accttcttca
    47221 ccttcactat cagcatcctg tgctgaataa aacccacctt ctttatcctt catctctcta
    47281 actacataat ctatagtttt tatagcaatt tctttataca actctttctt tgttatttta
    47341 tacgcatcta aaaaagctat agtaagcata gcattatcat atagcatttt ttcaaaatga
    47401 ggagccaacc actttttatc agtagagtat cttgaaaacc caaatccaat atgatcaaac
    47461 aagcctcctc tatacattcc atctaaagtt ttttccacca tttttaacac atctttgtct
    47521 ttttctatac tataatattt cattaaaaac ataagattct gaggacttgg aaatttagga
    47581 gcattaccaa aaccaccata cttttcatca taaatagcct taaatactct aactgatgaa
    47641 ctcaacatct cttttgataa atcaccctca gtatttttta caccaaaatc atccttaaga
    47701 gcttctatta tttcatctcc agattttatt aatatatctc tgcttgtatt ccacttttca
    47761 gatacattct ctaataaatc tataactcca ggtctattat accttgaata ttttgggaaa
    47821 tatgttcccg caaaaaaagg ttttttatct ggtgtcatta ttatggtcat tggccatcca
    47881 ccactacctg tcatagcttg gcaaactgtc atatacacac tatctacatc aggtctttcc
    47941 tctttatcta cttttattgc cacaaagttt ctattcatta tttcagcaac ttcttcatct
    48001 tcaaaagatt ctttttccat aacatggcac caatgacatg ttgaatatcc tacacttaaa
    48061 aatattggtt tatcctcttc ttttgctttt ttaaaagcct catcattcca actataccag
    48121 tttataggat tataagcatg ctgtaaaaga tatggagatt tttcatttat tagattatta
    48181 ggttttctat tactgtccaa aataccacct catttgttat tttattatat gttttgcaat
    48241 aaacaaatag atatacactt ataataagtg tatacccatt tttaactata catatttaat
    48301 ttttcatata attaattctt ctagtaaata atttttataa ctactaaaac aaaataaaaa
    48361 atatcaaatt atttttttaa aggtttttca aaagaaggag gattaatatc tatcttagta
    48421 tcgtgatttg gccctggagt agacaaatcg aaatataatt tagaatcttc tgtaactcca
    48481 aaatctctat ttgaacctgt atctttaact ttatttaaag catctctaaa tagagcatta
    48541 tgtgcttctt ctctattaag taagaaatct attgtttctt ttacatattt atcatctatt
    48601 tgtctataca agtactcata aactacctta gctctttgtt cagaagctat atttgaaagt
    48661 aagtcagcta ctaaatcccc tgtaactgta acgtaattag cagtccaagg agctcctgat
    48721 gagtttatta agactggtga tagaccagtt aatacatgtg tttctatttc gcctgtatta
    48781 actgcattat aatcaacatc atgtccattt aataaattta tagtttgtgc taccatttcc
    48841 atatggctaa gctcttctgc tgctatatca agaaataaat cttttatttg agggtctttt
    48901 attctaaaac tttgggacaa gtattgcata gctgctttta actctccatt tgcaccaccc
    48961 aactgttctt gcattaaaac agcatattga ggatttggtc tttctacttt tacttctttt
    49021 aataatgctt tatcgtgttt aaacatatga atacctccgt aaattattat tcaaaattat
    49081 tatttactta agttgtaata ttattctagg ttatttgttt attgttgtta ttttttacaa
    49141 gtgttggtat ttcaatgttt tattaaggtc agttttgttg tttatgtttt attatgttta
    49201 atttaaattt attctattat ttattttcgg tggcaaaagg gtggcaagtt tggttttgtc
    49261 accatttatt tttatttgta aaaaatatag tttatcaata taactttatt gaaaaatatg
    49321 gaaataaata ttgttttcgc cggttcggca tgttataata taaatataga aagggggtga
    49381 aaaggaaaat gagtagaata gaaagaagaa aaaaagatag agaaagcaaa ctcgccaaag
    49441 ttaaaactgc tctctctatc ttacccattt taataaattt aattgataaa ctaattaaat
    49501 tattaaaaga tttatagttt tgtaagctag gaagaattta atcttcttcc tagtgacttc
    49561 tttctaaata ttataacaca ttttctataa aatatgagtg ataaagttta taaattctgt
    49621 cttattatag ttgctataac tatagtaata gacataatac ttttattttt acattttaat
    49681 atcaacaata taataggatt aatagtaagc attttattgt taatatttgt tacattacaa
    49741 tataaaaaag gaggaaaata gtttggagca aaaagaatct aaaatagttt acagtaaagg
    49801 tggttcaggt acttattcgg ctaaaatttc tttaccttta agccaacttg aaaaaatggg
    49861 atttacaagg gaaaaacgta aggctgtagt tatttttaag gaagatgaga ttataataaa
    49921 aaaatctgat gaggaataac tctaaaataa gtgggtgtat aatgtaaaca tccacttatt
    49981 tttattatat tttgtatttt atattaatat attttataac tttattgaaa aatatggaat
    50041 atttattggc tttcacgtca cgtgatgata taatataaat atagaaagga ggtaagaaag
    50101 tggggagatt agaaagaaga aaaaataaaa gagaaaacaa atttaataaa attaaaaatg
    50161 ctttctcttt cacattagtt ttaattaatc tagtactcgc aatactaaga ttgattaaag
    50221 aattataagt ttcaatccta ggaggaaaat accttccttc taggtagctt ctttctaata
    50281 ttataacaca ttttctaatt taatatgaat aataaatttt atagaatttg tatgattatt
    50341 cttggtatca ctatagtttt aaatattatt aatataataa taagttttac ttttaaagct
    50401 cttattgctt tggtatttac tttattattg ctggcattta ttaatagtag aaaggaatga
    50461 tattattgga gcaaagaaaa cttagaattg gatttaataa aagtggcaaa ggctctttta
    50521 cacccagatt gatacttcca atgtcatgga ttaaagagat gaatatctct cctgatgaaa
    50581 gggatgttct tgttactttt gaggatggaa aaataattat tgaaaaaact gaaaatgaat
    50641 aaaaaaagga ggtagtaact atttccagtt actacctttc aatctattta actaagtttt
    50701 ctacttcatt aaatgctttt tcatttccat ttccaccaat ttgagttata tttttaaact
    50761 ttttattctt gattaactct ttttggctat tatccaatcc atttccaact aatactactg
    50821 gagattcagt tttacctgca agcactccta ctgataaggc atctactaaa tcgtcttgtt
    50881 tattcattcc attcttagct acatataagt cattaagtgt atctttgcta tagaaatgac
    50941 ttattacttt tgagttagtt tctgttctat cagaacctgc tattttagtt acagatggta
    51001 atgtattatt cacttcttta ttattatcag aacctgctat tttagttaca gatggtaatg
    51061 tattattcac ttctttatta ttaaataaag attctccacc tattacatat gattttgata
    51121 tgtttttatt agctattaag tcttttatat ctttcatatc atcattttga ttagtaagaa
    51181 gaattggcat ttcctctttt gctgatatag ctcccatact tactgcatca gctagtccct
    51241 tttctccatt taccactact acttttgaca ggttagaatt tttgtctaat tcttttgcaa
    51301 gttttattga tgtttcatat ctatcatttc cagatattct aattacattt aatccttttt
    51361 tctttaaatc agacactact ttttcatcaa cagactttaa tccgcccact atgtacacat
    51421 tttttgcttc taatctattt atttcttttt ctgttaattt atttaagtta ttatttttag
    51481 taagtaatat tggtgcatct ttagacttag caaatggtgt tgctgataaa gcatccgata
    51541 tgctagagtc atttattaac actatattat cagctttatt ccatcctttt tgacttattt
    51601 tcactgatgt ttcatttctg tctacacctg ttaatttttc cttagatggt gttgaagtag
    51661 gttcagagga ctcttcattt gtagatgatg aacctcctcc actccctgac gaacctcctg
    51721 ttgatgttga ttttttttga tttgaccaat ctaattcttt taattttgtg tttttgctta
    51781 catctaagct ggttaatcta ttttccccac aatatagcct tacaagttct gtattttttg
    51841 ttacatccaa gctagttaat tcattttgtg aacaatataa gtatttaaga tttttatttt
    51901 tacttgtatt taaactatct aatttatttt tataacaagt taaattttct aagtcaatat
    51961 ttttacttat atctaaagct tttatttcat tgtcataaca gtataattct aaaagttcta
    52021 tattattctc tatattcaaa tttgttaact tgtttgctac acactctaga tattttaaat
    52081 tcttattgct acttatatcc aaattcttca gattacttct actacaagtt aaatcttcca
    52141 aattaataca attttccaca tttaaagtac ttaattgttc ttgattacaa tatagtttta
    52201 caagcttttc atgatttttt aaatttaaat tttgcaattt attgttgtga caaattaact
    52261 taacaagttc tttattatta tctaaattta tattttctaa ttgattaaaa gaacagttta
    52321 aatcctgtaa ttctaaattt gtagatatat ctaaatcatt taaattatta taatcacatt
    52381 tcaaaatttt taatttctga ttcttagata agttcaaact agtcaattca ttttcaaaac
    52441 ataaaagagt tgtcaaatta gttgctttag ttatatctaa actctttaaa ttattttttc
    52501 tacagttcaa ttcgtctaat ttagaatttt ctataactaa gtctgttaga ttattttctc
    52561 cacaatgcaa cgttgttaac tctctattat tacttaaatc tagacttgtt aattgatttt
    52621 cgttacaata caattctttt aaatttatgt tgctacttaa atctagactc gttaaattat
    52681 ttcctctaca actaaaattt tctaatccaa taaaataatt aattccagat aaatttttta
    52741 ttgatttgct ccctaaggtt aaaccttttg tagattctat aatcgctttt tgtgaaattc
    52801 caattggatt tccatcatct aaaatactta gtttatcaca aattactttt ctaaaatttt
    52861 catctggaaa agcttcttcc attgtcatat tttctaagtt attagctttc cttgtttcat
    52921 atctaacttc cctatttttc tcaatatctt gtgcatttat cttatgtacc aaaggtgata
    52981 caactaccaa tgaaagtgat aaaataactg cttttttgct taaatttttc atccccacac
    53041 ctcattcaaa taatatttac ccacttgaat tgtatcacat aatattaacc ttagaaacaa
    53101 actttaatat tattatataa taaattattt ttttattatg aatgaggcat tagaagataa
    53161 caactacttt tagttgctac tttttaattt atctattaat aaaatctaat gccttataaa
    53221 gtgtatcaaa tctatcatta ccctttatca tagtaaattt ttctttagtc atagaaccaa
    53281 tcttctcaca tgctgcacca ccaatgacat aaagattttg cgtctgacct ggcacgtaat
    53341 cttttatatc acatatcagt attttcccat cattataacc ccaaccaact acagttgcag
    53401 agattttgtc aacttctcca tcataaacaa ttgtatgttt gtacatctgt ttaactccct
    53461 cattatttat atttttattt aatacacctt ctacaattaa cttagcaata ccttcatgac
    53521 ctagtttctt agctttctca taatcttctt tattatcaca gaagaaactt tcaattaata
    53581 ctgctgtagg atttgaacta tttaagatat ataatccttt atctaattta gctcttctat
    53641 ttctaaatac tgtacctagc ttattacata ttctagttgc atattctaaa cctttattac
    53701 tataatatag aacttctgaa ccttttcctt gaccatcact tgcatttaga tgtagttcta
    53761 taagtaaatc atatcctcca ctattaactc taggtatttt ataagtcttt tcttctgctt
    53821 tagttttaaa ctgcttttca gggcatatta ttacatctgc cttatgaccc tcttttctaa
    53881 atgtatctgc taatactggt gcaagagatt tattgtattg gtattcgtta actactccat
    53941 cagcagaagt gcatgcacca ctttttaaaa tactgtgtcc tactgtaata catattttca
    54001 ttatttattt tcctccttct tttcaataaa aaaacactta catatagtaa gtgtttataa
    54061 acttcttaaa gtaactattt tatttagcca atatctctat ccaattatta ggaaaaccaa
    54121 taagctttaa gtctatagaa ttattataat cattaattaa cttttgaagt tttataaaaa
    54181 aactattcca aattaattta tccatagtca aatgcttcat agcaagtata taagaaaata
    54241 tctttttatt atccaagtta tactttttat attcgttttt tattgttata atacggaaat
    54301 tattattata tattctacca taatgagcgc attgatttct gacctgtgtt aatgattgta
    54361 accaagattt aactaataaa gtattcactc tacacaaatt attttttatg tatctagtat
    54421 cttctggcaa catgtttgaa tataattttg acaacatacc aaaagtcatt atttccgttg
    54481 caacccaaat gggaagtttt ccttcatatt tttctttatg gtgtataata aaaagcttat
    54541 ctgaattatt acttttttct ctctctaatg ctgtcaaaaa attaatatag aatttttcat
    54601 ctttgaaact ctccctttct agatatccac aagcaccatg tttaattgct aatgtatatg
    54661 caatgtatgt cctaaatgct atttctatgc tccctaacaa atctgttaat aatatcctaa
    54721 attccttatt aaacctatat atatcataaa cttcttcaaa cgtagttcct tctttatatg
    54781 agccatcatc attcttaaaa cttagaagat atgctgtaaa cctataataa ttgacattac
    54841 ttaatacaaa tttagcatct tctttatcat ttattattaa tcctctactt tttaaaatat
    54901 ctatttgttc atcaaatgtt ttttcttctt ttacttcaac cattgttttc accttttcat
    54961 agatatactt ttatataata aaacccacca attaatatta gtgggtaaat ttatctgtcc
    55021 cgcctatttg agctataaaa gaagcttggc gggttccttc aaaaatcaat gtcccgtata
    55081 tttgagcata taaatgcttt acgagttctg tactattatt atatacttat tagctataaa
    55141 agtatactct atttaatgaa attttttaaa tttttgtgag taataatatt tttgttaatt
    55201 tcattacttt tcaccatcct tcaactgttt gtaagtttga tttataccta ttgcaactcc
    55261 ccaacaaatt acaccttgta agactgcaac aggatttaat cctagcatcc atattgagaa
    55321 acctattcca agtatcagta acactactgg aatgtattta ttatctaatt gtttatattt
    55381 cttgaatcca acacctaaaa cataaagagc agctactaaa attagcaact gctctggtat
    55441 aaaacttatt aaattgtcca tcttttattt tcctccaatt ctatttataa tttcatcaat
    55501 tctaagatgt gcttgttttg tacttgcttc tacttttgca actcgttcac tgatatctaa
    55561 gaaacttgta ttaaactttg ctacatcatg ctttatatct cttacattct cacacagaaa
    55621 agttatttgt tgttctaatt ttgtagttgt ttctgtatca tcttgtatct ttctgtttga
    55681 gctatttcta aatgctaagt aggatattac aactcctgca actgcacata atagatttat
    55741 gctaatttct tccatacatc ctcctagttt taataatata atttattatg cgaaacaagc
    55801 aatataataa cctgtaaaag gatatggctc ataactagta tcatatgaat gaactgaaaa
    55861 accagtttta ttgctgtcta tagtaatcca tgcacgttca gaacctcctg tattacttcc
    55921 taaagaacta ccactgggat tataataata tacacttctt ctaaagtaaa catagacaat
    55981 aaggtaactt gcactcttgc caaaattagc agaaaaagga ttaccatcat atccatcaat
    56041 aggaccatat ctaatagaat atttgctttt caattgagtt tctaaattgc ttatattgct
    56101 cttaaatcct gtaatatagt tcgcataatc ttgaaatgtt tgttctgatg tagcaggaga
    56161 gccgatagca gttgcaatta actcttttcc actatcggct tcttgaaaag cctcatctgc
    56221 tctatttata cattctttca atgcttcttc tacattttca cttgtaaact tattttctgt
    56281 atcttctatg gttactttct ttgcttctaa tacaagattt ctaactttat taactaactc
    56341 tttaaaagtc atttagtcac cttctttcaa taaaaaaaga accctctata tagttggttc
    56401 tgctccttct actactccac tattttttat aatataatcc tctatagcct ttctgtactc
    56461 tgtgttagtc acgtcatcta actgaaattc tctatttttc aatgggttta accctccgtt
    56521 taatattctc tctgctaata ttcttactac aacattattt atattcatta taatagtcct
    56581 cctgattttt cattttctgc aatcaatatt tgattttcta attcttgtat tctctttcct
    56641 tcttcactca tatatactgg tatttcttct aagattggtt gcttagttct tatatctatt
    56701 cctattattt tgtacttatc ataatcaaca taatcgtatt ccaaatctat atattctatt
    56761 ttttttatat tttttctatc tggaacattt cctttcgatt gtccttcata gaatattatt
    56821 ttgccttctt catcaaaaaa tattcttctt cctacctcta catattcagt catagtttcc
    56881 tcctttaact tattttttaa gcaattgcat agaaaaaatg tggtattttg taatctttca
    56941 ctggcaaaat acaagtgtga cctactacac aatcgaatgt gttaggcatg tacatttcac
    57001 caaaattgct aggttgaggc actgctgtta taatattacg aaaagatgag taaaaaacaa
    57061 aactacacaa acctatattg taagcatttc tatagacaat aataaatttt ggagtaaaag
    57121 ctaagttaaa acttattgcg tttgctgaac aagtttttgg cgtattatta tctacttgat
    57181 agagttgaaa agttttttct ccaacttgag tacattctcc ttgtgcaata aaaatatttg
    57241 cgattccagc tactttacca attagtgaat gaagtgtttc agttgattga gcagatatat
    57301 ttttcataga caaactaatt gctagtgtat ttttcaaggt ctgtattttg gttttagtta
    57361 cacttagctt atcagttcca acaaaaggat ttcctaatgt actagctata ttatttttac
    57421 caatttgcaa ttcatctttc acgttctcaa aagttgttat taagtcactt agactagcat
    57481 tttcgtttaa tttttctatc ataaaaatat cacctttcta tttttagtaa ctgtttgtca
    57541 agaaatggtt actttttaaa atagaaaagt gattgatttt aagtaatttt ttcaaagtat
    57601 atatagtata aataatttta tttttaatag aaaaacaagc aaaataaaca agtataaagc
    57661 tttttagtaa ctctttcttg acaaatagtt actatataaa attaaaaaag gactatacaa
    57721 tgtaatcctc tccaacgata tttttatatt ctattgttgt tatcttattc ttttctactg
    57781 ctgttttaac ttgctcttta gtccataatt catcctcata gtattttttt attattttat
    57841 accacatcta tatcaactcc ttttccatca ttgcaaaagt taatgttgct gtttcttctc
    57901 ttaatgcttt tacttcttcg tgtgtagcta tagttgcatt tttagaattt ttttctagtt
    57961 cctcatgtgc ttttatatct tcttcttgtt tcttatgcat ttctaaatat ttttctgctg
    58021 tttctaaagc agtcaaattc ttatctagtt tatatataga tttaattgta ttaactgact
    58081 ctatatttgt aactctgtta taatcatcaa gtattaaaaa tacttcactt gtttgaaaat
    58141 cataattacc ttttaaaatt tcttctgctg aaactccatt tattttttct tttttatccc
    58201 atatctttat cattttttat tttctccttt ttataattca ggtataaagc atttatacat
    58261 attattctca tagtaataga tatatttatt cttcactgaa actgctttaa gtagtggatt
    58321 gtatggaaat gtagaatttt ttttaacata ttctttagaa aatactcttc cattttgatt
    58381 gtatcctttt acataaagat atgtatcgcc ataatacaca gtatataaca tgtcattaat
    58441 acttgttgca aaacttttat accaaccttc tatttttgta agagctccat ttcctgcatc
    58501 atatctattt atataagttt cccaaatatt atttgggtct gttgctcctg ttatacaaaa
    58561 ataataggtg cctgtttttg cacatacaaa taatcttcta tctcgagatg gcggaaagct
    58621 tgttttagtt gaccaggtat ttgtactaat tttataatat tcactgatag cacttgctgt
    58681 tgaattacta tttgtatctg tgtaaccata caaaacatgt atttcattag aataagtatt
    58741 cccgcatgtt ccacctctag gtgtaatcat attagaaata tacgaccaag tatttgtttt
    58801 tatattatat ttttggcaag ttgaatttgc cgaactattg ctatatccac ctatcctgta
    58861 aatataacca tcataataaa ccattaaagc gtcatcacaa accgatggtt tcgctaagac
    58921 actattaaca tttgtcaatg tatcatagcg taaaagcgat aaacctaaac tgttattgca
    58981 aagaaaatat atatagtttt ctactgctac acatggagga tattgttgta atgccgaagt
    59041 aactccatca aaaattatat ttttaggttt ataccagttt ggtatgtctg tatttcctag
    59101 ttcaatatca tcaactttac ttaccatatt ttggacggtt tcatttgaag aagttgaaac
    59161 acctttttta gttaaattag ttgctaatgt actcttgaaa gtttgtattt ttgttttagt
    59221 agtactaaat gaatcagttg aaacaaaagg acttcctaat gtactagcta agttgtcttt
    59281 agcattttga aggtcatttt ttatattctc aaaagagtcc ataacttctt ttatacttga
    59341 atcttcttct aattttatag tcattaaatt cctgcctcct tttttaagta tcctttcgtt
    59401 tagaaatggt tacttaatac ttttgtaatc tttgaattta ctacattgta tattaaagta
    59461 aattaattta aaatataaag ctgtttttag aatagtttaa atttctgtcc cttttacttt
    59521 tttaagtaac catttctaaa caaatagtta ctaattttat aattctggta taaaacaagt
    59581 aatctcacta tttaaaataa aatatatttg cctaccatca actgaagctc caaaataact
    59641 ataattagga acagctcctt tatcaaaagt taatttttta attccactac tagaaaaaat
    59701 aacaactcca catttgccac ttccagcttt ataaccataa tctgatacat ataaagttga
    59761 acccaattgt acagcaaaag tattactatc aaagccttct cctgcataac tataagttcc
    59821 acttacaggg tcgtaattaa ttgttgaaaa atagtgagaa gaaatagaac tttttagccc
    59881 aaacacaaca attttataag tgctgacttt agcgcaaaca aaataatgcg attgttttga
    59941 atctattata gttgattttg tagaccaagt gtttaatgtt gtattataat attcaacaac
    60001 tctgactgct tgagatgaag aagaatcttc tccgccatac aaaacatgta tttgatttga
    60061 atacatttca caacaagcac ttcctcttgc ggtaggcata tttgctattt cagtccaaga
    60121 actagttaaa atattatatt tttgacatat agctgtggga gctgaatatc cactaaaacc
    60181 acctatttta tagatatatc catcataaaa aaatagcgct gattctacaa aggaagaagg
    60241 aatattttta gttgttgttg tgtttaaaac agtatcatag caaagattat ttaaacctga
    60301 tgtacctgta taaccgttta taaagtaaat ctttttttct gccgaaacac atggtgtttt
    60361 atctttaacc gcagtagaca aagctttaat aaatatattt ttaggaacat accaaggtgg
    60421 aacaatcatg ttgccttgct ctattttacc aacattttca gctaacgttt taaaaggtgc
    60481 attactcaca gttgatacac cttttttact tagatttgaa acaaaagtac ttcgtattga
    60541 acttaatgta ttcttagttg tttctaattt gtcatttcct gtgaaaggca aacctagaac
    60601 atcagctata ttatttttga ctgtctgcaa atcatctttc gtggtttcta atgtatctat
    60661 cacttctctt agttttgcat tttcttctaa tttcgttgcc atttttcact ctcctttatt
    60721 aaatcatatc tattaaatta ttcactatag ttattccttt agttctttga ccacttattt
    60781 ctaccattat ttcctctaat actccatcta acttatcact tgtaaatcta tcattagcat
    60841 ctgtaatact tatactggta tctataagct gtatactgct aatagaatcc tctattttct
    60901 tagacgaata agtagtcatt tcagacactc tgttatcatc cacagttgca ttaataaaat
    60961 gagtttctgc atttccattt atcacataaa cgtttaattc tgaccttgct tcacttctaa
    61021 tttctataga attatcatct ataattttaa agtttgtaac tacattttct tttgtagtag
    61081 catctataat atttacaact attctctgtg ttaataaact atgtgttaca gttgctttga
    61141 atccactttc tgcatcctca acccaatcat caattgcgac cctttgagtg tatgcaacat
    61201 ttgagccacc tgcgattaat tggtcgattt tagtatttaa ctctgtttta gcagtttcta
    61261 tgttgcttgt taattctgtt ttagttgtat caattttagt attaacagta cctattttag
    61321 tttctaagtc ttgtatatct ttgagtgttg caaagattat tgttgggtca attttaagtt
    61381 ctatattatt tacattagat acaataagca cagttttaac cttcatgtct accactgcac
    61441 cttgttctat agaaggttta taacactctt tgtatttaga aatggcaatt aaattatttt
    61501 catcatctaa atatcctatt tctcttatca taaacccgcc tacacttgat ggtattaaac
    61561 tctctaatat tatacaattt ggtgcagttt catctgtagt tgtatttcca atattgcctt
    61621 cccataccac gtttttgaga gctgtctgac tctcagttgg agtatattca ctccctcctc
    61681 catcaccaag ttgaattttt acaaatccca ctttattacc tgtgacactt gcatttgcta
    61741 tctttgcttt ccctacatct gtaattatag tgtaataact tttatctata gccaatatat
    61801 cacctcctaa aatattgtta tctcttggta tccaactcca ttgccagtta atacatcaat
    61861 ttctccataa gtttctatat ctggtggact ccaagggtat atagttattt cttgacctat
    61921 tagggttgtt ataccaaaat tcatataatt gtctttactt acaagcactc tagtgtaatc
    61981 taaagtcatg ttgcaaggtt taatactact tacaaaagaa tgaacctcct caaaccaatc
    62041 ttgatttcta gcatcacttt caagatgtat attataagta gcattattaa tagttaattc
    62101 ataattgcct tctccaacta tactatctag ccagttcctt aaaaatctct ctgagtaagg
    62161 tagtttactt atatatttac taaaaatcct aaaccttcta tcttctaaac tctcattact
    62221 tttaggagtt atagacatta tcttttccca tctttttata ccacttggag ttaaatcctc
    62281 taaaaactgg tcacttgata ggtcatttaa tttttcatgt agtgttttta tttctttgtt
    62341 ttctacatta aatactttta tatattcttc tttatcttgt agaatttgtg gtaagtaatt
    62401 tattagatta atctctttat ccaactacct cacctctcac tactatactg ttactatcta
    62461 ttgttagatt agatttaacc tcatttatca ttgtatttgc aatgtctaat actccatcaa
    62521 tactaagtaa tctagtttca atttgagata tacggactat taagttttct tcatcttccc
    62581 aactcatgtt aagttcattt aaatagtcgt ctattgcttc ttctgcaatt gattttatat
    62641 tctcccaagt gtagccattt ttgtatgtta tctctgctga tatatttata gttgtacttg
    62701 taactccttc aactgtgact cggtgtccaa ttggtgctaa tccaagacct tctccttggt
    62761 tttgtagagg atcaatttct tcttgaacta aattaactaa atcactagat ggaactttga
    62821 agttagaatt aattattact aacttaacag tacctccacc gtcccacaca ggataaacct
    62881 taactcctcc aacatcttgt attttgttaa cttcatcttt ataattttgc atatttccac
    62941 caaaactctg tgagtttagg ctatcataat atctttgtct taaactatct tcactttctt
    63001 catcttctcc attaattagt atttcagtta actcagcagt ttctaatttg tctacatatt
    63061 caattggaat caactgacca agttcaaata caggtccagt agtttcacat ttcattttat
    63121 atgttttttc agatattctc tcaattgcca cataattgta ttctcctaag ttgaatcttg
    63181 aatcaatagg aatatctata ttaaaaactc cttttgcaat tgtattggtt gcaggtaaag
    63241 gtgtaatacc tctttcttta catctcttct ctaaatagta ataactagca gtatctacaa
    63301 aagtttggtc tagtaattca tccatggcaa tgtatgtttc tgtaagttct atagcaacag
    63361 gagcaagagc attatatatt atagaacctt cccttttatc aaaagtatct ggtacactat
    63421 ctaacattct tttaattata ttttcaaatg tcattaactc aaacaattat acactcacca
    63481 ccttctctgc ttttatattt ccatatttac tgtaaactgt gaacttacaa tgtactttac
    63541 cctttacatt ttgaaactca aaattatcta cattttcaac cctatcatct tgaattagtg
    63601 cttctttgat tcgtctttca agttcgggga ttacaaagga tataggctct ccaataaggt
    63661 cgttcaactc gactccataa ttccaactat atattagatg ttggtatctc tctgtgttta
    63721 aaattaaaaa gatggtttgt tttaatgctt caacatcatc acaaatacca tctatcttag
    63781 atttttctat atttaattta aaggtcttac ttggttcttg ccttacatca aaattaatta
    63841 ttgatacatc ttcaatgtca taatctaaat tatcgcttgg taacacttca tcacatccta
    63901 tccaatacta aatattgttg tcctccttgc atacgaatta agactacttt atctcctatt
    63961 tttttatctg tatatctttt aaatgtatct gtttgtatta gaaaaatttc accaatagat
    64021 agtttttgtt ctatcttaac tcttagagga ttaatacttt ctattgttcc aaatacaacc
    64081 ctcattgggt tgcttgtttc tactgcatcc attgcagctt tttttattat ctgtaataaa
    64141 tcttggctca tattgtcacc tcacttatat aaatcttctc acatgtgtgt atgcttttcc
    64201 ttttctataa gaattaacag actctatttt taccacatct cctgtttgtg gtgaatgaat
    64261 tatttgattg tttccaatat acattacaac atgattacta cttcctccac caattctaca
    64321 taataagtct cctgctttcc acttgcttct atcttttaaa tctactgctt ttcctgcttt
    64381 actttgtgca gaagcggtac gaggaatttt tatacctatt tgtttataac accattgagt
    64441 aaacccactg caatcaaaat tattaggacc ttctgctcca tacacataat tacaacccag
    64501 tttacttttt gctatactaa ttaatttatc ttctttagag ttattatttg tactactttg
    64561 gttattacct tcaacttgat atgtttgttc ttcatctcca cctataatta tatagccatt
    64621 ctttctacca aattttttac attcactagc attagctaat agtatatcta tatgatatgt
    64681 tccgtttgtt tcaacatata ttcttcctcc attatcttta actgtatata ctttgttgtc
    64741 ataggcagta ccaggaagta taatttttac tttatctcca tattcaaaaa ctggatgttt
    64801 ctttagaaaa tcatcagtat accaagtttt cttaactcct tctcgattca ttggaccagc
    64861 aacagttctt gattttacat caagtggctt tccattgcaa tctgtttttc cgccttccat
    64921 tgcattgttc cctggataat atgcagtaaa tatagcagga acttttttac ctgtattttt
    64981 tttcgtaata ctttgtgcag gaccattttt cttttcatct ttattattag tatttccact
    65041 actatatgag cttgaagaat atgaagcaaa ttcgtctcca tcaacaaggg tcaaatccat
    65101 aaagtgcgaa ttattttcaa atgtatgttt tactttctca actaacatat aattttgtaa
    65161 ttcaatatct cccaaatcta aaaaaacagg tactaaacaa cctgctctca ctctaatatc
    65221 accaagtaca ttttttaaac ttaatgactt agttttctta ttatatagtt ttagaagtat
    65281 atcacacttt tgttttatct ctgcttcact catgtttttg tctactgtat caaacatttg
    65341 aagtattccc caactcctca tatgcgttga gtcttgagca atatacacat ctctttttcc
    65401 tgtttcttca ttatctctca caagtttaat ctttgtgtaa gtatcactat ctattgatga
    65461 attatagtca aaatcttcta ttacatcatt gttcataaca gtatctaatt tcattgatgc
    65521 aacattcttt aatgttattc ttccaaaatc atcatataac acatacattt cttttttctc
    65581 tcttagagta tcatctagtg ctgttagtat catgtcaaag agtgttttat tttcttcgac
    65641 tctagatagt ttatacttag tatcttctat gacattgtat tttaaattaa aatctttagc
    65701 caacattttt acaagttcac ttgctgtttt attgctatac acataagtat ctttgttctt
    65761 aaaatatctt aactggtcgt aagcaacaat tttaatgtga ttttctttat ctcttttctt
    65821 ctgaaatata tatccataga atatacctac acctttgtaa tatagtctta cagagttacc
    65881 ttcacaaaac tctaatatat catccatgac tattgtaaat tcaagcttag atggtgttcc
    65941 tcgtctctct atttcccatg tgataccatc aatgacagca ggttcataga aatcttccca
    66001 atgtgctatg attaatctta catctctatc attcgccaga actaaatcat cagccaagtt
    66061 ttaacacctg ccctttgtaa atagtgtatt tacttaagtt tttgccctta tttgccttat
    66121 ccatcataga tttatttagt tcgtatactt tcttatataa tgaaccatta ccaagttgct
    66181 tcttgcagat taaccaaagg ctatcccctg cttttactgt atatgtttta ccatttggct
    66241 tattggatga atctggtcta aattcttttg gtttcatgac tggaggggga gtcctaccat
    66301 agtttgtctt ttcaggagtt gcaggaacta actttttagt tgagtaatct ctatattgct
    66361 ttaactttat tgcaactttt gtatctgagc cattttctgc atcttctact atgttatatt
    66421 cctcaagaga tactttcata ttagtgttaa atagtacttt attacctaat tccctcgata
    66481 caataaattg aaatggctta caatcagttt ttagtaattc tagtttactt aaaaagaatt
    66541 gaacatctct aaattgacct ctacaaaaag gtaatttatt atgtgtaaat tctgcttcaa
    66601 aacttatttc agataatcct tcttttttta atatatttac ttctccaaca tttattaaat
    66661 caactgtctt gtttttattt gtaactttaa tctctaactt tggcggaggt attggtaatt
    66721 gtactccatc taaataaaag tcataagcca tttatatccc tcctctctaa actattccct
    66781 cagctgatac aaccatagcg tcgtttaatt tttctgttag tacatttact attccatcta
    66841 catctgcctc actatttatg ttgtttgtat tgttcatgtc aattttaatg tttactcctg
    66901 tgaatctatt tattgtttct tgctctgcaa tatctctaag atattttaag tcttcttgac
    66961 ttttatccat tgttttagcc attttggcag tatttcccgc cgtgtccttt acattttttc
    67021 ctaaatctcc accaaatcct ccaagattat taagtggatt gtctttatcc cacaaatctt
    67081 ttaatcctag cttatctttt gcatcttcta gcatcttatt aatgtcaaaa gtatctttta
    67141 atttattaga tatagcattt tgccatttag ttccaagtgc atttcctttt tgaaattctg
    67201 ctccaatatc tttgtatccc attcgctcca attttacttt ctctggtgcg tctcctaccc
    67261 atctgtttaa gctatcaatc tgttgtttga tgtaactatt atctgcctta actggcgtaa
    67321 acgttgcctc tcctacttta ccaatattta tccctggtat tttatttatt aggtcaatta
    67381 acttatttac ccctcttatt gctatatttg ctccatccac aaaagcctta ccaagtgcgt
    67441 ttcctgcatt atttacagaa tcattcaatg atgccatttt ctcaattata aaaattacac
    67501 cttttgcaat tgcttgtttc atcaaatata cacactgatt ccatccattc gcaattcctt
    67561 catttacatc tacacatcta ttaaatagcc atatcattat attttgtatt gttgcaactg
    67621 ctgcaaatac tgcacctaca atcaccccta gaacagtcaa agacgtacct gcaaatttat
    67681 ttattgccgc tactaccata aatattacag ctattactgc tataaatcca agaactatcc
    67741 acacgagcgg gcaagcatac aacgctgcat ttaggccata ttgtgctgct gttgcctggg
    67801 ctgttaccat cgcttctata gtttgtgcag atattttacc aaataaagct aattcttgtg
    67861 ctgccaatgc tgcacaagct ttatatgccc ctaaagcata cagggcattt acaaaagatg
    67921 atactccagt tacaattgca cttgcaagta aaattccttt atatattgca atagctgcga
    67981 caactccata aacagcaggt gcaataatac tccaattttg agcaaagaca ttagcaacat
    68041 ttagtgcagc attagtaatc catcccaatc cttgtattat cagtacagtt cctgttgtaa
    68101 ttccttgtac aaaatctgaa aaaaaatcac tatttaatat attttttata atatttaatg
    68161 tatcataaat gtttgaagat attgcaatca taacattccg catagataca acaatctcac
    68221 taaaacgtac actttgcaaa gctccgctta tcttactgag agtctcagaa aatatcatat
    68281 aagcatcatt tttcatcatt gtgagagctt gagaaaaagt gattggcata cttgcaaatt
    68341 tcttttctat ctcatcagaa gctttaaaca gtgcatttct tataacatct gctgttattg
    68401 ctccgctact tgataattcc ttcaattggt ctttagtttt tcccattgca tcagctattt
    68461 tagtggctaa taagggtgca ttttccatta tggaacggaa ctcatcacct tgaagttttc
    68521 ctgcacccat ggcttgtgtt aattgataca ttgctgcact agcttcattt gctgatgttc
    68581 ctccaattac aaaagcttta ttcataagtt ctgtaaactt tatcacctct gctgaacttc
    68641 caaaagcatc tcctgcaagt attccaagtt tagcaatctg tgctgctgta tctgcataac
    68701 tagctcttgc actttgggct gacaaataaa tcattttatt aagctggtct gttgtctgta
    68761 agccatcatt cattagattt aaacgtgctt ttgtacttgc aattgtatct gctgcttttg
    68821 taatactttc tattcctttt attccaatgt atatcccagc aatacttttt aatttagaca
    68881 ctaatagaga acctgcttca ctaccttgcc ttattttatt attaaagttt tcctgctcat
    68941 ttgtatttct tccaattttc tgttctatcc ttgtgagaat actttctatg ttattcaaac
    69001 tttgttgaga agcttgtata ttacctgcat tgagtggatt attcaatctg ccttgcaatc
    69061 tctctaaact attaattgtt gtattaatag aattagtcat attacgaaat gctggtgtca
    69121 ttccgtcgaa aatctttatt gaagtttgta ttgtagccat ttcctcactc tcctttcata
    69181 aatttccata taaaaaaaca cctacattag taagtgttta ttatttatgc ccaaaactgt
    69241 ttaccacagc tcaaacatgt aactctaact ttctttgctc ctaaatttcc agctactaaa
    69301 ccaatgcctc ctgctatagt agcccctgct acagcttttc ctatgccaaa acctttctta
    69361 tgagcagaca atgaaacaga gccacaccta gggcaacgag caacatcatc ttcaagtaca
    69421 ttagtttgtt tatactcctc ttccctcaat atctcttttt cctcttcgat atttattttt
    69481 tcacttaatt ctttaaaagc tggatatata atatttttac actcttttat gtccagccct
    69541 agtaacttca ttgcaatatc aattgctaag attttatctc ctttagtttc tttataaata
    69601 ttagtcagat ttaattcttt tccatgaaca tttataaata aatcatcatt gttaacttta
    69661 tctgctaatg tagctgttcc gcaatttgag caaaatttgc ctgcaccagt tatctcataa
    69721 ccacaatttg aacaaaacac aacaatcacc tcacataata cttattttat agcactatta
    69781 tactatatac gtaaaatttt tacattatta tcacctcctt tcataaaaaa aaacacttac
    69841 tcatttgtaa gtgtttttga attattttta attttaagtc cacatagtta atataaaacg
    69901 agcatcattt atcattgcta atcgtgcatt tgtacattta cataccacat agttaatata
    69961 aaaccataca ctagattcta ccccaaaaat gaagctcaat atatttacat accacttagt
    70021 taatataaaa cgcgttaatg cagtaccata acgatatggg actaactaat ttacatacca
    70081 catagttaat ataaaaccgt ataggaatga atgcaggtag tgcattttct agtacattta
    70141 cataccacat agttaatata aaacgcaacc cctaaagacc ttctaaaaaa attactctta
    70201 gatttacata ccacatagtt aatataaaac acaggtgtat ttacctatag aacaacaaga
    70261 gttacattta cataccactt agttaatata aaacattcaa gaagtaactg taattgttca
    70321 tctgtaaaat aatttacatt ccatatagtt aatctaaaac ctggtcttaa aaacatacat
    70381 gttgcaaaag tgaatgatga atttacattc catatagtta atctaaaacg aacactaaca
    70441 aaaaagctaa aagtggtcac tatattattt acattccata tagttaatct aaaacgaatg
    70501 atacattaga atatatattt tgtagtgaat gatttacata ccacttagtt aatataaaac
    70561 catcctactt agatactgca caaatagtat cgcgaatctt tacataccac ttagttaata
    70621 taaaattgac cagtacgaat gcgaggctag caatgataaa cgactttaca taccacattg
    70681 ttaatataaa aaccttgagt gacgcagcaa aaggggcaaa ggatacagca tttacatacc
    70741 acttagttaa tataactcct acctatatta tatcattttt tactacataa agcacttgaa
    70801 acagtaaaat attcaagtgc tttatttgtc tatttatttt tctctttttt tcctacattc
    70861 ttctttcact aaacttacaa atctataaaa ttcatctgga ttttcatttt tcattttttc
    70921 aaagatattt ccaagttctt tgataagttc tattctatct atatcaagta aattctcatg
    70981 cattgtatta atccccccaa aactaaacta aaatatttaa tacaactgat aaatttactc
    71041 aattttataa accacatgat aattcttctt ctcacctgca atcttggtag gtctattatt
    71101 ctcctctatc caatttctta ttttatctat tacactttct gtatacttat ttacagtacc
    71161 agtccaactt ccattagtct cccaaactcc cttaacttca ctatcttcta aatcaatctt
    71221 tttaataatc tcacaaacag ccatctgagc tggtttatta ctcttagaat atagttttaa
    71281 cttagatgct atttgttttg tgtcaaaata atgttcttct tcgtttatct ctattggcaa
    71341 atcaattcct gcctttttat ataatgtttt agctgtaagt aacttagatt tattgtcaaa
    71401 acctgcacca tccaatagtt cttttaacat agatgtgctg ttataagcca gttgtaattt
    71461 ttcaatctca cttgcttttt ctcttagttt ttcggggtca gcattatttg ttatgtatgc
    71521 accagtttgt cgaatggctg gaagtacttc atcacttatc caatcttgaa atctctcagc
    71581 ttcttctttt ttagatttaa atataagttt ataaacacca ctttcagtta gaaaattttc
    71641 tcctgtatta tgcaattttc taaactgcat attatgcatt ttagaattag tcaacttaac
    71701 tacttgatta tcattaaatt ttctaatact actgttcaca tctgaaatat ctaaacactc
    71761 tgccacatgc tttggattaa ataaaatttg cccttcaaat tcgaatacct ctatatcttt
    71821 tccttcaaaa ttcattaatt cattcataat aaattcctcc ttaaatttga ttgtaagaag
    71881 taccttacta tgatagaata tatttcatag aaggtaactt ctttgggaaa cagtcgcaag
    71941 tgctttggtc ggtgcagcgg ctgtttttta tttgtttttg tcaatcttct ctttcaccaa
    72001 ttcaattcct ctaattacaa catctgtttt agatatttta agattttcag cacactcatt
    72061 taatatatct gcttgttctt ggttaagtct aacttcaaat cttaattttt tggaattttc
    72121 ttttggtggt ctgcctaatt tattggacat cttatcacct ctctttttat tgtccgtact
    72181 taaattataa tatagtacgt acaataattc aagagttttt tactaatttt ttctaattat
    72241 tttactcaac cgaccaattt tgagcaaaac aaaagcacct accaaaaagt aagtgctttc
    72301 tatataacag atttaatttt aaaataaagt attcacctaa tttccagacg agaaatccaa
    72361 atcatcaatt aaatttacat tattttttat attattccaa tcatctaaaa atacattata
    72421 agtgagtgtc acatcaacaa taaagcttat tctcactgaa tggatatcat tcatatcttt
    72481 tatattgttt acaaattctt tagtaaagtt tctacactca ttttcatcat taaacctaga
    72541 attttccact tgaatactaa ctatatatcc ctcacctttt gttgggttta atatgttaac
    72601 ataatatgtt gttttatctt ttaagtcttc tggtatcata gagtctactt ttgcttgcat
    72661 ctcttgttta taaatatctt ctttttctct ttcgctaatt atattttctt ttgtaatatt
    72721 tccttcattt acttcatctc tattttttga atctcgtatc gcttgatgaa tcatcattga
    72781 tactgtaaat acaacaaagt atattaaaaa tatcactaaa attttcttca acaaacttaa
    72841 ttttttaaat tttccccaca taatgaactc tcccctatta aattctttat tcaaatataa
    72901 ttctatctaa aaataagaac ttagtctatt gatttaaaat attttgagca aaataaaagc
    72961 acctaccaaa aataagtgct tctttcttct atttagtttt tctccacatt gttaatataa
    73021 aagtttggag ttttaaaatt atcattccta tcttttgctt tatttacata ccacatagtt
    73081 aatataaaat tgaggtagaa aatgataaaa agacttttag agtaatcttt acataccaca
    73141 tagttaatat aaaacctgtt aatgtaatca tttcaccatc ataagttgcg aaatttacat
    73201 tccatatagt taatctaaaa caataattgg tgatggtaca ggatatacaa atgcaacatt
    73261 tacattccat atagttaatc taaaacagaa aattcattgt acaattcttc tttgtatttt
    73321 ttaatttaca ttccatatag ttaatctaaa accccaaaat aaacttagta tttccaatat
    73381 ctacacatac acacctctct caaatttgca gtgaaccatg agtagtgcaa ttgataacat
    73441 ttatcacaca ccctcaatac cttgtattcc aattgttaaa cttcaattta tcgcaaatat
    73501 tgctcactgc aaaatctcta catttttatt atatcataaa aatattattt ttgaatatct
    73561 gtaccaattt gtggtataat aaaagcaagg aaataattta ctttatacaa gagtagctat
    73621 ttccatcaaa attgatttaa agaattattt ttttaaatca cccttattgg cgtctgggtg
    73681 attttttatt ttgtcataaa tataagctga tataacacca gctagtatgc ttaataaaaa
    73741 aactatcata taatttcacc tccttcctta tttggaattt ggcgtttaat atgaaaataa
    73801 tcacccttcg cactttcgat tattatcctt gctacaatta ttataacata taattattac
    73861 atatttttcc atttttttta tataaacaat gaaattcaag taaataaata cctacttatt
    73921 tatatatatt ttataaatta attgctttat aatcaagttt tcaatttttt aataaaaatt
    73981 tttatttttt attttatata aacaatattt tttctaattt gtggtataat aaaagcaaga
    74041 agaactacaa tctattttgc ggtagagtga agttcataat taaatgaatc tatttgaact
    74101 tatggaactt gattttaaaa tcaaattccc agccactttt actcttgcca cgagttgagt
    74161 ggctttttac ttttagaaat actttacaaa ttaagcaaaa tattaaacta gcaataacgc
    74221 cagctattac attaagtaaa aagttattca tacttcccac ctcctttcat taggaagtag
    74281 gttttatctc agtatgaact ccactctata aattgtagat tacatcttct tgctaaaaat
    74341 attataacat ataattatta catattttac ctattctata tttatttttt tattttgcta
    74401 tcttcttcgt cccctctttc tctctctttc agcttctttc attgcttcct cttcatcttc
    74461 tatctttata agtattgagg cggctgctaa cgctctctca ttaacttcta aattcatata
    74521 ttcacttggc ttccacttta atttttgaat acaataatga gtgatgctag catcaaaatc
    74581 gccacctctg attagttttt tgcttcttct actttatcct caaaagatgt atcaaatcca
    74641 ttgacttcat tcacttttac tgtataattg acatactcac ctgctgtaag cattgtcttt
    74701 aataactgag cttctcccat tactccataa ctattttgga gttcggcatc ctttaaatct
    74761 ggaaatactg tagatgctac acataattca gctacataac tattgtagtc aatttcactt
    74821 gtatattgtc cagtatgctt accattgtta ccaatcactt ttactctttt agtacacttt
    74881 cttcttagtg cttcgtcttc ttcagatgat aaaactttta attcccattc aactggtttc
    74941 ccttctttat ctaaaaatct gttactcgct acatatttta cattatcaac ctttattgca
    75001 ttttgactta aaaaagcact taaattactc atattattct aatctccttt tattttaatt
    75061 tttcatataa aaaatacaca tatataattt ataaatgtgt attttactcc attcctgcca
    75121 ataaattaaa tttttctact aattcccaat cctcaaaagt gaaatccata tcttcatcta
    75181 aatactcacc atcagcatca aatttagtaa ttattccact gtccatatta caatctttaa
    75241 gtactactgt ctgtcttcct acagcagatg tagggtcttc atttgtaact tgtatgtcaa
    75301 aataaatatc ctcaccagtt tctttatatc tgtaaagtaa ttctctaaaa atagaagtat
    75361 tataatgaaa tgttgcactt ccagtatttg tactcccagt tgttttattt ccctttgttg
    75421 ttcttcctag aattggaact tcacttttat ttttttccat tttagcctct aaatctatag
    75481 cttgcataaa attatatctt ttgccttcta tagttataaa acattcagct ttctttgcac
    75541 taactgtatc tttagcattt attgtttgag ccatattatc actcccctct ctaactaact
    75601 gaaactgtca tataaagctt actcatagca tttataacct taacagcatc agatactatg
    75661 acagttttct tatcatttcc aagctctaca ctaacatcat cagttttaaa atcttctatt
    75721 gcccttatat tctctaattc tttatggtgt ttaacaacat cattccagaa acttattctt
    75781 cctgccttat cattcggaac tttacctaaa tacttttcat taaataaagt tgcaatatca
    75841 ttagcaattt ggtcaagtac tctaacactt tggttacttg aaaaatcgtc atttttatca
    75901 tctgtaaatg atacaaaagt atttatatcc tctaacacat gaacttcatc tcccaccttg
    75961 tggaatataa atttaccagt ttttaaagct tcttcaagtt gtatttgtgt ataattaaca
    76021 tcaacatcaa actcaccatc atacttttta ttagtattag atttatttat atcgcatcct
    76081 gctatagctc cagccgccca ataaattaaa ctagattcaa ctaaatcttt atctttaatc
    76141 ttattttcta cagacactac accttcataa tctgcatcac ttttcttata tagtactgtt
    76201 tgaaacttag ctcctacctt atctctcatt ctctttgtaa attctacaaa taaactttta
    76261 atttctgttg ttgtagccaa acatcctaaa gcattaaagc tatagctttc tattttatcc
    76321 aagaaagctt ggtactctgc tcctgtcaca gcttcgccat tagttccacc agtaaataca
    76381 agtcctgcac ttgcttctag tgttgcatcc ttcttccaag tgatatagtc attgtcttgt
    76441 aagtctgtaa taacctttgc tatttgagta tctaccttct tattatctaa aagtgttaca
    76501 acatcaaatt tagtgttatc atctatattt gttgtaactg ttacttttaa gtcatttcct
    76561 ctgataccac tatattttgc tgtggctata gtacaactgg ctttaacgcc tttatttaat
    76621 ttataaaaat atcccaacct tatatttttg aataaatctc tcaaaccttt cagcttctca
    76681 tgagtataat catatccaaa atacttcact gaatacttct caaaatcatc actggttact
    76741 tgaaatacgt ctttatctat gccccaatct aactctaaag gtattgcaac aatacctcta
    76801 tccgataatg aactggttgc ccttgtagca cttacaaaat ttatatatgc accaggtagg
    76861 accttatttt gtgttacaaa tgttcctcca cctaaagcca tctaactcac tcctttcata
    76921 aatttattta ttctatcctc tacctctgag aaggaatata aatcattttc ttttaaaatt
    76981 gcatttaata aatcttttct atttacatac ttcttagagt taactatctg ctccttagta
    77041 aacttgtagt tatcttcttt gcttaatgtt ttactcaaaa ttatcacctc tcttcaaacc
    77101 accgaataac tctactgtat ccatcttatc tgtgtcatta ctttttatag taaagtagtt
    77161 ataatcaaca aagaaatgaa gaacattatc tataatttca aagttcatat ttgtacctct
    77221 gactaagtct ccatcaattt ctatatactc taattcctcc agtaacatct cagctatctc
    77281 atttatttca aatgatttat cattactttt aggaaaataa tgtacatcaa aagagttctt
    77341 ttttaatgtc ctgccactta gataggatac tttgcttgga tttaaaggaa caataaaaaa
    77401 acaaggttca tttatacctt gctccacatc ttcactataa attgtataac tctctccaaa
    77461 tgttttatct aatttaatag atattccatc aattatatta ttaagcatca aatactcctt
    77521 taagcaatat taatagtttt ttctctataa tcttatcaac ttggctttgt agttccatct
    77581 ctgaaattgt taagaaatgt tgtcctttaa cccaaccttt tccatcttta gttctatggc
    77641 catattcaac ataacttgca tatgtggtcg gattaacaac ctctataata taattatttc
    77701 cttgtttata cacaggaagc gacctagcat aagccactcc attccatcct tgtcgtaaga
    77761 atcctgtatc aactggtgtt cttctaatta ctttcccaag taatcgtgct gctaattctc
    77821 ttgctgcatc cttgcaaaac ttatctaaat caatctttgt aagctcctcc atctttttac
    77881 aaactctttt aaactctcta aaatcaacac tgccccatct agccattatg ctttatcctt
    77941 aaataactca agtattattt cttgatgatt tggatatata gctgattctc cacttcttac
    78001 atactcttta tcatttataa taagttttga acctgcttta atttctatat ctggagatat
    78061 aaagagttta atagtttgct ctagcttagc tactttccct tctgtagcag aaactatatt
    78121 tttacatgaa agtttacatg gttgattttc taatacaatc acttctttat tgttagttcg
    78181 ttttgttaca gggtctttaa ttggctgata ctcaactata gtacatttat ctctatataa
    78241 catttctatt gcttttctag ttttacttac catcttaagc acctaaaggt taatatctta
    78301 ttcttaccat aagtagtaag ataagctact aagctatcaa agcgttgttc tggtgtttga
    78361 gagccacttc ctatagcaaa atctaccttt gtatcacctt ctgatataga cttttctaca
    78421 gcttcaaagt taatgctttc tatatctaat tgccccatat ttttcttggt aaataagaac
    78481 tctccaacta tcatatcaac ttcaatttct ttcaattcaa ttggcatagt ttttatatta
    78541 caatctagtt taataatatt ttctattttt tctcttacaa aatctattaa ccacttatcc
    78601 ccatctttta atatatatcc aaaactttca agtctttttt ctatatcatc aattatatta
    78661 tttcccataa ttttcaccta ctttttagta agtttatttt tctctttaag ctgtttattt
    78721 tcttcttcta aagactcaac ttttgacctt aaaatattat tttcagctat taaatctttt
    78781 acatttaatg acttgccata ctttactgcc ttaccagttt catctatcaa atcatatccc
    78841 atctctaaga aatcatctat tttacattct tctatagtta atattctatt taatttcctt
    78901 acttgtgcca ttatgctcca gctccttcaa caacaaattg tattgcatca gcttttttat
    78961 ttaatataaa tacatcctca aaactttctt caaagtagaa gtattttccc tctgtaactg
    79021 ctgttggttc gtctaactta gagaactgat aagaaacagg tgtaattatt gcacttgggt
    79081 gaactaagga cataaagatt tgtttagctc ctgctcctac tttccatcca gttgtaaaat
    79141 catatgcagt tttcattaga ttagatggta ctttaattat tttaactgtg tcaatatcag
    79201 ttgtttgacg attaagagaa gttcctgcat cctttatatt tactgttctt tgtatctctt
    79261 ttgcattttt gataagtgta tttactactg gagtaacata caatattctt ccattttcag
    79321 gtactctagc ttctgtcatt ttttccatta acttatcaaa tacttctaat acgtttgttg
    79381 ttgtaagaac agttgtatct gctgtgttac ctaatgcggt ccaatcagca tatattttag
    79441 atatacagta agcatccatc tctggaaact tttgttcctc attatatact tttgttatat
    79501 tgcctattga agccacataa ttagtttggt ttatatctgc tggatgaacc aatgttgacc
    79561 atttcctttg attagttaat accttaggtt cccaagcatt atcatagttt ctttgagcta
    79621 ctgctattgt atctctgttt gaatctactc ttccagttgt agatatagtt ggtatttcta
    79681 ttgttttaga accagtccat ctatatcttc cattatttgg tgttgcatac aaatccccga
    79741 agtttaaagt ataaggatat gcttgtgcta aaacatttga atattctttt gcataattta
    79801 gtgctgccat tttatttcct cctatttatt attattttca tgaggtctta ccccagtaaa
    79861 attaaaacca aaatcattta tcttaggctc ttgccctggt gttatagtat ctattttagg
    79921 ctcttcacct tctagtgttg cattaaacaa ataatcttta tcctgtttca aagggtttat
    79981 ttgctcttca aaagcttttt gtctatcttt actatttctt agtgcttcta tatctaaatg
    80041 agcttttaac gctatttcat ccctacattt aatagactta aaagcatcat ttaaccaata
    80101 attaaagtcc ttttcttcaa tttctttttt gtaggtttct tccaaagttt tcttatcagt
    80161 ttcataagtt gtttttagat tctctacatc ttcttttgtc atacctcctt caaacttttt
    80221 aatagcttca ttagctgtat taagttgtgt ttcaagattt gtataatctt cttgagtaat
    80281 tgtagtctct tttattttct tttctataga tttttgaaga gaaactacat caattttgtt
    80341 attttctatt tttattcctt ctagcaattc ttttaaccaa tccatctttt aaatatctcc
    80401 tttcattttt tacataataa aagcacctac taatttatca tttagcaagt gcttttacat
    80461 atttactatt tgtatatctt tccataaatc ctttagtaat ttaccatcaa tgttgtaatt
    80521 atcaaccata tccttaccat ttttataata ctttgtatct ccattagggc aaagagtaat
    80581 aaaccttgta tcatcatctc caatagatat attatatggt ttattatata agtcaaattc
    80641 tatatctaat cctaaatcaa tagaatcaat taaatgttgt aaattcttaa atttattatc
    80701 cattttactc tcctttcaaa atatctttgt tagctatttt atgagctttt gtaagctcca
    80761 tatccttctc tctttttacc ttatcatggt tattttcatc agctaaccaa tcatgtttat
    80821 gaggtacaat tttatgttgc tttgggtttc tatggtcagt taagtctaaa tctaatctag
    80881 gttttcctgt attaccatag tatcttcttt gaattaattt accatctttg taattatcaa
    80941 atacactatt tggttttgat tcaaatggca ctgaatgaac acttccacta gttaaatttc
    81001 tctgattctt aacttgccaa tttacatcct tataaagctt tttagcttcc tcatacctta
    81061 tagtatcatt atacttcata tgttgatatt catcaaattt agaaggtatt tcatttccta
    81121 ataccttttt atattcttca aattgttttc tatctttact ctcatttagt tgcatttttc
    81181 taagggtatc agctttttct tttccaagtc taccctctat atgtttctca taccactcat
    81241 tatacttcat attagatggt acataatatg tttttccatc ttctcctttt gctgctctgt
    81301 aaccttcttc atcttcaaac caaggagctg ttgttgtcct acaacgacaa tgaaatggtg
    81361 gagctgttat tccaacttga taatctttca tatcaaatac tttaccatct aattctctgc
    81421 atatatttga agttcttaaa tctagtgtag caattatctc atatttctct acatctaaat
    81481 cattaaaaca atcttttcta cttgctgatg caaagaaagc tgattcagtc attattaagt
    81541 tcttagcttg agatttagat acattaaatc ttttagcaaa gtcatttact agattctttg
    81601 gattttcacc tctaataatt gattgagtta gcttagtatg tagttcattt actaaagcat
    81661 gtctatgctt accccaaatt ctttcactaa agtttaatcc atctgttgcc catggtttag
    81721 agataatttt atttattcta ttagtatcaa gactcattaa actccaacca acgtttactc
    81781 cttgttgaac attaaaagct gtatgatagt atccacttgt ataaatatct ctcattagtt
    81841 tatcaatacc atcaagttca tttccatata aaacttctac ttgttgctgt atttgtaact
    81901 ttaaagcttc aagccttgtt atatgaactc ttgcactagc attttctaac tctttcatcc
    81961 acttttgatt tatagcattt tctttaccat atttaatata ttcttctaca ctccatttaa
    82021 actcttctag ttctcttgta tttagtagtt tcttagcttc taataaagat attccttcat
    82081 ttttggcaaa tctgttgtac catgctaata tatctttttc tatattattt atagctagtt
    82141 tatattgctt ttctaattca agataatatt ttacactttt gttattttga gcttcttcta
    82201 attgttcaaa tctctttctc caataatctt tatgtttcat ctataacacc atcttgatta
    82261 ttaggaatta aatcatcata ctctttttga gtatcttcct gttttttaag tctctcaagt
    82321 tcgtcattaa catcctcaac ccaaggatga ttggaaacaa tagtttcatc tgatatgatt
    82381 ccaattgatt tagctgccat atctattttt tcagactcat ttattatcat tgagtggtta
    82441 aaagtaattt gaactgattt ataatcataa cttttactac cacttatctt taaatactca
    82501 catacaaacc acaaaagttc tctaattgct tttttaaact tcttttcagt tttggagcat
    82561 tttaaatcaa gtagtgaata taaaaattta agtgctacac cagatttgtc acctgtgttt
    82621 tgagattctg gattaactcc ttgaccaaag ataattatat tcttttccaa tctatcaaga
    82681 agctcctttt tagcttcaac tggtatattt atctctagtt tatcaactcc acctccacca
    82741 tctactttaa ttgatttata gtatcttata ttatctataa actcttgtag acttgttcct
    82801 ggatattctt ttaatacata aataacctct tgtatttcat ctaagttatc tgctagtgta
    82861 gaaatattat tgtcatatat atctattaat gatttataga aatttaaatc tgatacgcac
    82921 ttttcattat ttttaaaagg tataaatgga actttacccc atccctgttc tttgttattt
    82981 attctaaaat gaccttcttg tatatcagtc atttttccat attcatcata taaaaattct
    83041 tgaataaaac tattacctct ttcaataaag taagttacgt cattttctgt gtagtactca
    83101 actcttttta ttttatttcc atctatatct tcaatataat aaaacctaat aaatgcaact
    83161 aattccctct gtcttttact atcccaaata ggaattgctt cttcagcagg tattattaca
    83221 tatttaaact caccttttct attaatatat ggatgtaacc attcaatacc tttgttacta
    83281 gcattgaggt agagttctgt tattgtatcg tcaaattctt ctcctagtaa gtcatttaaa
    83341 agcttagtga gattatcatc atctgcatta aatactatgg gatttccgac actatagcct
    83401 accttttggt caactaaaag cttatggtag ttgttaattg ctttattatt aactttagta
    83461 aaatcatcaa ccttagctcc atctaagaga taatatcttc tcttattgtt tatatctgca
    83521 ttgccataat agtattcttc accttgttta tatttctctg gtctatgttt taaaatataa
    83581 tgctctataa cttttactag gttaaaggtg ctctcttttt ttaactgaac ttttattaaa
    83641 tctgtttcac ttatataaat atttaacgcc tcctttactt taagaagctt attccattat
    83701 ttttaagctt attatctata gaatatctaa gcgcagccat tgcatcatcc ataaactcaa
    83761 ctggttcatc aagatataat ccagttcttt catcttgttt ccatttccat tgttgtattt
    83821 cttttatggt attagtgcaa ctaggatgta catgtattct taattgtttc aaataatcta
    83881 tttgagcttt aacacttcct ggtccttttt taactccttt agcttcatat cctgcactct
    83941 tccacatctt aattctatct ggttcagcac tatcacagta cataaataga ttcttttcta
    84001 aacctttatt atttgcaatc tttatgattt ctgaggtatc catttcatgt acatatattt
    84061 cgttacatat atataattct ccatccttaa agccaattct aagtactaca tttgcatggt
    84121 taaatccaaa gtcttgtgat aacctcatat tgtcaaaata ctcaaattct gtaggaaatt
    84181 catgtataac ataattttta agtattgctc caccagtttc tccccattct ccaagaccat
    84241 agactttgta cccttctggg tcttgctctt ttctcatttg cattcttctg tagtaagcct
    84301 catctatgaa tctattttgt agataagtac tatgatgagt aaatatatca tcatttttat
    84361 agtcaaaata ctttcttttt atccaatgag tagctgagac tggattaaat gtaaatgtca
    84421 tttgatagta taggttagga ttagttaaaa tacctcttaa acggtcatct agtatgtcta
    84481 tgtcactttc cataagttct gtagcttctt cacaccaaac ccatgttaat tttcctttcg
    84541 agaagttaat tgattttaat ttttctcttt gttttgcatc attaactcct ctgaaaatta
    84601 tagagttacc agtaacttta ctcttaattt ctaaaggatt taaagtagtt ttccaatact
    84661 tatcagcttg tttactataa atacgattta tagctcctgt aagctctgca tacgttgaat
    84721 acttatgtgt agcttctgac tttctaacta ctaatagatt agctccttga tacttcttat
    84781 ctcctaactt tagtatatag tcttgtgcta cattaacaga ttttccactc cctgctgaac
    84841 ctttcattgc tctgtatctt tttttagtaa aattagcttc cttgaaatct ggattaaaat
    84901 ttactctaac tatcatttct atcaccataa tctacactta ttttcaactc atcatctcca
    84961 atatcatctt tacttaggtt atcaacttca catttcaact tctcaactct tgttttctgc
    85021 tcctctgtag ccaaattcca atccttatga atcatttcat catactgttt aattaaactt
    85081 ctaagttcac tcatagctct actctgtgca ttaagaaaag atgcttgcct atcccatgca
    85141 aattgaaatt catactctat cttttcacca ttttctgtgc tttcatattt ctttaattct
    85201 ttaaccattt cttccttgcc tttaacatac attatctttt gtgctcttat tattgctgca
    85261 tattgaattg ttatctgttc ccaaagaata tcaaatttat cttttataga tatttcttgt
    85321 atcaattctc tagtttcttt gggtagatac tttgagaaga aaccaaactt ttcagcattc
    85381 ttattctctt ttggagcacc atgaccaact gaatttttat tagaaaaggg tgcacctctt
    85441 ttatttatag gtgcaccctt ctttttttca ctagcccagt tgtatctttt tatccatgac
    85501 tttaaagtgt ttaaactaat gtcatacttt gatgatattt ccttttgttt cattcctttt
    85561 aagtaatctt gttttacctt ttctttgaca tcttgcacat caccacctct ttatttgttt
    85621 gttttgggaa taaaaaaaca atcctattta ataggattgt ttcaaatcta gtctcctcta
    85681 tccttccagg ataaatgaaa catacttatt attaatgtag ttagagataa aattatatga
    85741 actgtaaaaa atattaaaac atagaaaatt atattagaat aatatcgagt aagcattatt
    85801 gtattgataa aacatattac aacaaccaaa atctccatta ttacagtata atatgctttt
    85861 aactgtatga attgaaagta tgatatatta aatccattta cctttctttc tgtttttata
    85921 tcttttgctt ctgatattga tttgttatat gtagtaaaca ataatgtaat aatgcaaact
    85981 ccaaaagcta ctaatagtcc tgacaaagtt agtacatctt tgtttaaaga aattacaact
    86041 tctttattaa ttgacgaatt aaaaaacatg tatgaaataa tacatattaa tactggtgtt
    86101 aaaactaact ctactacttc tttagaattt atagccttgt aataattgag taagatattt
    86161 aatacctttt tatacatttt aacacctcct tcgcaatcta tgcttcactc atagcaactt
    86221 cttgatacat taaattaaaa aaaactgaat tattctcaat taaatcatct atcaaattat
    86281 tcatcttatc aaaaatatct tcactatcta tatgattatc tatatcaaga gaaacctcta
    86341 tttctctaca caactttata ccctcagtat ccaactttaa ttttctcttg tttttctctc
    86401 cctcaattat tatcctattt atttttttat ttctaaattc ctctccttta atatattttt
    86461 cgcaacatct tttaacttca ctaggtaaat atctagttct aaatgtgggc ttatatatca
    86521 aatcgttatg tcgtcttgaa ttatttaaat tagaaaacaa attatcttca tcagttccaa
    86581 acttctctct atcaacattt aaagttattt ttgaaacacc atctaaatct aatatttgtt
    86641 ctataaaatc tttacttgct atttgtgtaa tattaattga tgtttttgac atattattat
    86701 caaatttatt attttcaatc atatcctcat aatatagatg taagttttta cttagttcaa
    86761 tcatgggaac ccccacaaaa actctttcaa ataatactac tgctatatta ttttccttca
    86821 taaatttaat aaataggtgc tgcttattgc tatctccttg atccattttt ttattaccct
    86881 ctacttcttt agtatgagca tttataatat ttgtattctg attgtattta gtatattcaa
    86941 gtttcacctt gattattaaa tcatcctcgc tacattttac ttctgtaatc caaaactttt
    87001 tatctttagt aatattaatt ccatttttgt tttctttaca atattcttta tccttaaaga
    87061 aggtctttat tacctctttt aaaatttctt catctgaata gacactccca ttaattttat
    87121 ttataatatc tattctataa acatcaatat atcttttttt aaaattactc attataccat
    87181 tattcctttc acaaatattt ataatgatat aattctatat aatcagtcaa tatccttcaa
    87241 taatcattcg acaaccacaa aataagtcta aataatactt ttaccttatt cctgtctata
    87301 atgtacaaat atatttcatt tttaattcat tacataaaaa aaagaccctc cattaataca
    87361 gtcatttaaa tcatttctat taattcttta atcttttata tacctcttta taattcatac
    87421 ttttatctat caacctaggt gatttcatag atataactct ttcaagtgct tgtattaata
    87481 aacaaactct tgtatttcat ctgtaaaatc ttttctgacc tttttacctt ctgttcttat
    87541 atccagccat tcttgatctt tttctgtggc aatatagtaa ccatgtattc taatttgttt
    87601 aagtgctttt gtaacccact tcgtaaatag ttttgcttct gctttattac ttctaaatga
    87661 catattgtac acagcttctt ctgtaacaaa agtagtacca aagttaggca atttatcttt
    87721 aaagtttcta gtgtaggaat ctccgacagt agactcatta atttcttttt atattctcta
    87781 tctatatttc ttaatgtatc acgaatattt actatgccta gttcctctcc tacgtcattt
    87841 gcattaaatc aaacttcttc tccatttttt gaccatatca tccttacatt tttctcttgt
    87901 aaaattttca acatactact acctcctaaa ttagttatta ataaagaata ttcaatttga
    87961 acacccttat tttcgaccct tcaatatgag ggattaaaat tgtataaaaa agacctagaa
    88021 atcactctaa gtcatgaatt actcatttat cattctcctt atttacaaaa taaaaaagac
    88081 taagttgggg taacttagcc tttttcaagg gggaatatat tatacacttg tttcatacta
    88141 ccattataac agggttaaaa tatcattaaa atatcatctt tttatcatat atttatcaaa
    88201 cagttaattt caagccatct ataccgaata aatacactcc cagttccttt atcatttcat
    88261 taatccaacg tcttgatgta actactccac aatttaatat ctctgctatt tcttcatatg
    88321 ttttttcatc acaatagtaa agctctaaag ctctatactt ttcataagaa tgtaaacgct
    88381 cttgtcttaa tttcaaagtt ttcattgctg aatctatatg tgctatcata ataatagttc
    88441 ttgctttact tttctttata cttaaaatat acaactcttc attatcaagc ttacctaaat
    88501 catcctctaa gtaatctaca tctttagctt cacttaccgc tgagtctata tgtctcttaa
    88561 aatcattata atgcttcatc aataatctag tattatataa aacgcctttt ttcctatctt
    88621 ctctttcttc ttttcttaac tctcttacaa tttcttttat actttcttta tccacttaaa
    88681 tcactccttc ttgctctctt cgaacaactt ctactgcaat acttaacttt agatttatcc
    88741 aacttataaa atcttctacc acaccaagca catcttacta ttttacctgg gcttataagt
    88801 tccatctttt ctttttactt tcataccttc actccctata ttcttttaat tttgctttga
    88861 ctgcttcaag taatgcactt tgtcctttat ctttattctc tagagcctcc attacttgtt
    88921 catctattgt acctttacag attagatgat ggataataac agtttccctt tgcccctgtc
    88981 tataaagcct tgcattggct tgttgataca gttctaagct ccaagttaat ccaaaccaaa
    89041 caattatact tccacctgat tgtaaattaa gtccatgtcc tgtacttgct ggatgacaaa
    89101 gtaataattg tattcttcca ttattccaat catatatatc ttttgagttt tctattgttc
    89161 ttggtttcaa actcttaaac tctttcatta aacgattata atcatgttta tagttataaa
    89221 aaactataat aggcttacca tttgaaacat ctataatttc ttttaaagct tctaattttt
    89281 ctctatggag ttcctttaca tttttatcat tatcataaat tgctccatta gctaattgta
    89341 aaagtttatt tgctgctaca gctgctgatg aagctgttat tatatctttt tctaattcta
    89401 atatcttctc tttttctaat tctttgtaat atttaagtat tttactatca aggtgtattt
    89461 caattttatt atctattttt ttaggcatat tcaaataatc ttctgctttt aagcttatac
    89521 aaatatcttt tattttatta tgtattgcat tttctgctcc atctttaggc tgccaattat
    89581 atatagctcc tgtttgataa tttttttgtc ctggctcaaa gtatcgttct ttatatcctg
    89641 taatagtttt acctagtctt tcacctctat caagtaagta catctgtgcc cataagtcta
    89701 ttaaactatt tggtgttggt gtaccagtaa gcccaactac tcttttagtt aaaggcaata
    89761 ctttctttaa acttttaaat cgttgtgctt tatttgattt aaaagaacta agttcatcta
    89821 tcacaaccat atcaaatggc cattttcttt tataaaaatc cactatccag ggtaccattt
    89881 ctctatttat tatataaata tctgagtctg tacttaaagc tctcactcta tctaatttac
    89941 taccaagaac tttagatact tttagatgct ttagatgctc ccattttttt acttcactac
    90001 tccaggtatc tcttgcaact cttaaaggtg ctataactaa aacttttgat atatcaaagt
    90061 aatcatacat taattcactt atagctgtta gagcacagac tgttttacct aatcccatat
    90121 ctaatagtaa acctatatta ttattatcta tagtttttct aattgtgtat tcttggtatg
    90181 gatgtggttt aaattccatc ccttaacctc cttgataaaa tcatctatct cttttaatgt
    90241 gcttacacac ttaactttaa atcctaattc tcttaattct cgcattttat attgttgaag
    90301 ctttcttagt tttttacctg gtgctttaag ttctacaaat ataacatgtc cttctggtaa
    90361 taagacaatc ctatctggca cacctgcctc acctggtgaa ataaacttca tagcctttcc
    90421 acctaacaac tcaatctctt ttttaagtct tttttctatt tttgattcta acaaaatatc
    90481 acttccttaa aatttaagtg tcaacaatgt caacagattt tctatatata tgtatatata
    90541 catattaggc atacatatgt atatacgtat atgcctaata ttacttatac tactttatac
    90601 ttaatttttg ttgacattgt tgacattaat acctcaaaca ttgtaattac tgcatcttag
    90661 gtgtcaacat aagcgtcaac aaagccattt tttttgttga cattgttgac acttttaaaa
    90721 ttttcaaatt tactctattt ttaattctgt tgacactaac ttttacgtac ataagctctc
    90781 tgcctaccat atattttccc aaaccttaaa tttccattat atgatatcca atcatctaat
    90841 ccttttaata tatcattaat ttctcttgat aagataggcg tgagttgttt aggttctcca
    90901 ttaaatagct caacccatat ctccataaca cacgtttttt ctcttaatat tgtcccttct
    90961 tttaaatcac caaaatctga accatgaata tattctctct tttctgaaat acttaaatca
    91021 taccaattct tagtaatagg tttatttaga tactcttcga taattccaga tttagcattt
    91081 tcttctgagt gagttctttg ttgtctttca gcttcttttt tctcctcatc tgatagatat
    91141 agctgttcat tggctttata taattctact gcttctgccc aaatttgatt tctttcatta
    91201 tcaagttggc cattaaatat actcttgtta gtcttcttta ctcctgcatc tattggccaa
    91261 aaccttctat tcccagtctt atctcttaaa aattctttat cattagttgt tcctataact
    91321 acacattgac gtaaaaatct tgaggttctc ttcccatatg cgaccctata tatatcctct
    91381 gatttgctta gaaaatgctt aactgcctca atatctgctt ttttagtagc catcatttca
    91441 cccatttcca gcaaccatac tccttgtaat tgttcatacg cttctttacc ttgtacagta
    91501 gttaaactat cagaatacca gtctccacca agttttttaa taaaagtact ttttcccatt
    91561 ccttgaggac cagataaaac catcatatta tcaaacttta ttcctggatt aaatactctt
    91621 gctactgctg ctactaaaac ttttcttatt atagtccttg tatagtggtt atcttctgca
    91681 cctaaatagt caattaaaag tgtatccact ctcttaatgc catcccactt taaagaattt
    91741 aaataatctt ttataggatg aaaagtatta ttttcaaaag caattattag agcatcattt
    91801 acttttgatg gtgatgagat attgtaaatt gtttctatat gatgtctaag cccagaatca
    91861 tcactatcat tccaatcatt taatttatca tcttttctcc aaggtaactt acctaaaacg
    91921 acagctctat ttgaaaattc attataagct atttttcctt tcaaatatgg gtcattttct
    91981 ataaacatta agatattgtt tgttgttttc ttataacttc ccttattgtc ataatctaac
    92041 ctagttaacc attcatcatc ttcaaaatct atatcaccaa aatcatcctt agctttatca
    92101 aggttttctc ttcctatagt ctttcgtact ttagtgtcac tgcttgcaaa ttcgctcatt
    92161 cgagtaaatg aaggtaatct atttacaggc gtttcgggtt tagcatcttc atcaagttca
    92221 ccaaatttat gtatcctaac taaatcaaaa gcattgcata aaattccgct tgctgggtct
    92281 gtaccatgat gactatatga aaacttgtca tcataaataa ctactccacc acttgtacta
    92341 ccttctgaat atgtatacct ggtttcatca ataccaggaa tatatacttc atttaagaaa
    92401 gtttctatag tttcttttat actataggac ctgcaaaatg cacctataat tccagccttt
    92461 tcaatagggt cttgttgttt ttttaattgt gtattaaact tttgcctctc cctcgaactt
    92521 tctggccaat agcttacatc tgtccagtct aaatataaat ctagtatttc atttgggttt
    92581 aaaaactctc catcttgaaa tttaaatata tagtctccat caattgaagt acttggaaaa
    92641 tacataagtc tatgaggttg atatgtagta tcatcaaaca tatctattcc tatagtatct
    92701 gctatcatcc tggaaattgc ttgatattct tctggtagta ctggtctagt taaaggtatt
    92761 acaagtctat atctgggatt attctcagta tgtgagtgcg tagaatacat aagacaagca
    92821 taatcattta ataatgttat atcctcccat atatctttat tcgcataatc tatatctaaa
    92881 gttataatac ttctatttgc aatattctct gcttttcgtc ttccattttt taagcttcca
    92941 cctacaaatc caccaacatc tttaactcta tctttttcag tctttgacat cttcctatat
    93001 tctgtatatg tttcttgagt tcttaatgtt ttacttaatc tatttacaag ttcggaccat
    93061 aaaatacttt tatttttcca atgtgtttct aatttatttt ttcctatggc cagcatgagt
    93121 tggccatcat gtcttacatt tatgtgttca atttcactgg cctttatatc cataaatcaa
    93181 tcacctagtc tttcttataa taatcacatt catatccatc tgctttaaga ggaagacctt
    93241 tagcccaaga tatttctttt cccataatac tgttaacttc ttctaaagaa cctccttttt
    93301 tatctacatc aattacaagc tcatcatgta catgcattac aatgctataa cctgcatctg
    93361 tcacattaaa catagcctct cttaagcaat ctctagctgt agcttgaaca atattctcaa
    93421 ctaacttagg tccataagta tctattcttt tccattgttt acttgtctgt tccatacctt
    93481 catatgttat cttatcccca ctaaatgtag tatgtggctc tatcttaggc cttagatacg
    93541 ataatcttct actacttggt agttctataa ataaaacgcc tggattataa ataaatttaa
    93601 tcccatgttg aatttctact atagtcctat cttttatagc ttttttagct gccttatcta
    93661 catcccacca aaacttagtt atatttggat tagcatttct ccatgcagta acaataggtt
    93721 gaagctcttc ttctttaagc cccattttaa tagcccccat agaaattaag gcccctacac
    93781 ttccgccata tccattcgat aattctgcta gctttccttt ttgtctaagt tcagaacctt
    93841 ttttaatatt ttcgattgga attttaaaca tctgactggc actagcttca tatatttttc
    93901 catgagaatt aaacacatcc agtctccact tctcacctgc aagccaagct ataactctag
    93961 cttctattgc actaaaatct gacactataa atctatgacc ttcacttggt ataaaagctg
    94021 tccttatcaa ttgacttaag acatctggta cactatcata taaaagctct attaaatcaa
    94081 aatctccttc ttttaatagg tttctagcta ggtctaaatc ctctatatga ttttgtggta
    94141 aattctgtac ttgtactaat cttcctgccc atctaccagt cctattagcc ccataaaact
    94201 gtagtagacc tcttactcta ttgtcattac ctttagctaa tttcatagcc tcatatttct
    94261 ttatagaagt tttggacatt aattttctaa gttctaaaat tctaactaca ttttcatcat
    94321 caacttgttt taatatttct ggaatacttt cttttgttag gcttgtaatc tcaaagccaa
    94381 ctttatcact taaccatttt tttaattgag ctggactatt tggattattt agaccagtta
    94441 ttttaattgc ttcttttgta agtttttcag tatatctttt atcacattct attgcattct
    94501 ctattaactc tgtatccact ttaataccag tatcattaat tctctggtct aaataccata
    94561 atttaatttc tctttcggta gtcttatact tactaagttt gtttcttatt tctctttcaa
    94621 ctacaacatc ttgtttacaa tattctttaa atttattcca tttttccata tcatgtattg
    94681 gtaaatttct agttcttccc ttgttaactt ttgtagcctt acaaggttta cagaaatatt
    94741 gtattaatgc tttaccttct ttcattttct gtttatcttc attaaacttt aaagccttag
    94801 acacactatc taaacttcct ggaagtccta gtgttaacgc ctttatcatt gtacatgacc
    94861 actcatttgg ttttaaatta atatttaaaa atttacttat tgatgttctt tcaaaattag
    94921 cattaaatgc tgattttata actttattat catttaaagc ttctattact tcttttggta
    94981 actcttcatc atttactaaa tcaataactt ttacctcttc attatcaaaa gcataggcaa
    95041 ataacagtat ctcaaaatta gcagagtcta catatctgta gactccaact ttttttatat
    95101 ctaaatcact atatgtttct atatcaattg ataaggtcct cattaactta agaagtcctc
    95161 ttcttcatct tcatattcaa agtcatctga gaaatctgct tctgcactag ctctagctcc
    95221 tccaagtact tctccatctg ctaacttttg aacattttgt aagccacaac ctattccttt
    95281 atttcctgca ctattatatg ggaaaaagtt tatactaact cttccataac aaccactata
    95341 tacctctgta ttatctaata tttcatttaa gtctttatca actattcctg gcttttgagt
    95401 actatttgca tttaaaaaat acattcctac atattcctct gcttcatcag ctctttctgc
    95461 atctccatca cgaagaggtg tttttaaatt acctggtaat tttccacccc atttagaagt
    95521 tttaccttgt tctttagctg catcaatagc cttctttatt cttcctaaag tcaccttatc
    95581 tgattttggt attaaaatac aaactgaata cttgggctct gcaccttcta ccattgctct
    95641 gcttttaaag atattgcaat aacttaatct tacctttcct gttactacct ttgttgattg
    95701 tactgaatta ctcataattt tcttcctcct aatattttta tatttggtat attgatttta
    95761 tatttgaagt tatttctcca aatttatcta aatctctaac ttgcttacta aacaatttaa
    95821 ttcttccaca aataactgtt gaagaaacat ttaataactc tcctatatca gatatctttt
    95881 tatcttgtaa aatccaatct actattttat caatatcttt taaatatgac tttcttgcaa
    95941 tttttcttat ataatcaaca tcatagttaa attgaatttc ataaaatcca tgtatatctt
    96001 ttaatggtct tttactatct tcaaatacac ttacatctaa accttctata gagtttctat
    96061 aatgtttttc gccataacgc aaagctttaa acatttctat tcttatgtaa gaaacagcca
    96121 ctgtagaaaa cttgcctctg ttactatcat aatttaatgc tgctttatat aacccaatac
    96181 atccttcttg ataaaactct tcatataagt atggatgttg ctctacataa gacttaaaaa
    96241 atctatttat actaaaatgt acaagattca aattattttc aactagttta gttatttctt
    96301 gattactcat aaaattatat ccccttttaa atctttttat agctactagg aattaaatat
    96361 ttttaattat cctttgctct tactgataca aagcatactg gactaacttt tatatgttct
    96421 ggtatataca ctctatcaag atgattgtca tttacttcga ggaaagttga catcacttta
    96481 tcctttatta aactttctct tttaaaatcc atcactataa cttttgcatt ttcttcagtt
    96541 gagttatatt ctgtaatatt tagtatcttt aacatatcta gtaaattatc tttttcataa
    96601 tctaaatctt tttcgatact cttttttcta tcctttaaat ctaaaattat acttatgctt
    96661 tcatctaatt tatcttttat acctgcatct aatggcatgt aatatccccc atttatattt
    96721 caaaatcttt tttcgctgaa tctatactat taatttctga ccttttatca ctttctacta
    96781 ctaaagtagc ttttccaact ggttttataa ttaaatcact tagtagttta gcaaaactct
    96841 ttttacctat agctttctcc atatcactaa ttccttttaa ggtccttggc ttataaattt
    96901 tttcttcatc ataatctgaa tttaataaaa ccttagcaac ttcttgctca tctacatact
    96961 ttctattact tctgccttct acaagcttat atcctggata tttaacacca tgcttttcag
    97021 cttgttctag tgcataactt tgaacatctt ttaaccaatc ttgtatattc ttagcaaaac
    97081 ctaaaatatc tgctatctca tatttattaa gagcaaatgt gtcagcaaaa tcatattttc
    97141 tagctagttt aaggttatcc tcagctcttt ttctgcaatc atttttagct ctacaaaatc
    97201 cacaatgact gccacttaca aactctcctt caccattaaa agccatttga gccttcttct
    97261 taacattatc tgcccatgta agtaatttag taacttctat ttcttcactt gatatgttat
    97321 caagtctagg ttgaattatt gttgttttaa ttaaatcaat atcgtatagc atttcaaact
    97381 gattatatgc ccctaaacca tatagtctaa gttgaggatt ctctatagct gaaacttcta
    97441 aaccttttcc atactttagg tctataactt gaagtatacc atcagatatt acaacaacgt
    97501 ctcctgttcc aaatccttct ggaacccact cactaaaatc gagtctttct tctaacatca
    97561 ctattacatc atcacatata gctttactat catttactaa ttcaactaca ttctcaacat
    97621 aagattgtat atagtcctcc atttcagagt tataatactc acttttctgt atctttttaa
    97681 ttcttgcatt atatgccttt ttacttattt tttcatactc tagcattaat ttaacttctg
    97741 ctaattcatg tgcaactgtt ccttcttctg catatatact agttgatggt ggatagtttt
    97801 cttctaattt tatactagga gtgcagtgaa gccatctatg ggctccactc gcactaagtc
    97861 ttgcatgttg taatggcatt tatgtacccc ctataaactt tctaatttgt tcataaaagc
    97921 tgaataatct tcttctttta cttcacttaa cttactagct ccaaattcac caaataactc
    97981 cttaagctta tccttcttac ctgccttact tacttgtgct gccttagttc ttacctcttc
    98041 ttttgtgtat ttaacttcac tagttgtatt ttcattattc ttttctattt cttcctttgc
    98101 ttctgctatt tcttctttaa cgtcttcttt tacttctacc tttttaatct tttcagcttt
    98161 tttaacttca actttcttct cttctttcat atctgttata tttataggtt ctatctgcat
    98221 tgcttttcct agattaagac ctcctaaagc attcgccact actaatagtg catttgtaaa
    98281 ttctggtgct tctactttaa cttttacatt tacattcact tcaaccattt taaatctctc
    98341 ctttttgtgt tatactttac ttgtgttata ttttattttt tatttttttg tgtgttggtt
    98401 attttaccaa cactttttta tttaatattc caaccgatat tttcttacca gttttaatat
    98461 ctttaaatac tatatctgct ataacttttc catctttttt aagagttact acattcttat
    98521 tattagtatc aagactcagc aatttcatcc cccctctcta ctgcttttaa aagttcatcc
    98581 aaatttttac cttgatttct ttcaataaaa tcatcaactt catatcttga aatttttcta
    98641 ccatcacctc taactagtga ttttatcaaa cctgtgctta ctaacctacg cataaaagct
    98701 gtatctaact ttaaaattcc ccttgcttct tctactgtta ttagataatt tggataacct
    98761 ctttttatta aaacaactat atctttaggc tccagtacct ttaccttttg ttcaatagtt
    98821 tgactcttaa ctctatctgt ctcttgttta cttatctcta tttctattaa gctttttaag
    98881 ctatcactaa attgttttgt tatgctttca gaaatatcca tgtgaaaatg ctctcccctc
    98941 ttttcaatta tttatcttcc aaccaattcg tctaatgtaa tatctaaata atcagctaat
    99001 tttattagag tatctatagt tggattagaa cttttatttc tcagtaattt atataaacca
    99061 cttacatcta tatccaattc ttttgccaat ttataaggtt ttatatctct ttttcttagt
    99121 atttgcttta tattgtcgcc tattaacatt gtattcacct cagttctaca tggtataata
    99181 taaacatgga ataaattcca aaattttcaa aaggatggtg tattatgcag ttttcaaaca
    99241 atatatctaa agaattacaa gaaagtataa gaaagacagt ctctgatact tctaacacag
    99301 agtattttta ctatgctgaa tttcaatata aaattattct taaatctata gaggaatttg
    99361 aaaaagaact tgatgatgaa catgaaattg cactaaaatt gactaacttt ggaaaagatg
    99421 tattaatgat tgttgaagaa gtaggttatc ataacccttg tctaattcac tactacggca
    99481 tagtaaatgg agtttattct gaaattcttc aacacacatc acagataaac tttatgataa
    99541 cttcagtaaa gaaaactgac ccttcaaaac ctgctagaag aataggtttt atattgtaat
    99601 tatcattctt attttctgat tctttcttta gtaatttcac taatttttct tttttcactt
    99661 ttactcatct catgtaaata caaaactata cattggtaca tataatctat aaaatcacta
    99721 tttgattgag ctgttatctg actaaataac tgtgcttgca attgattttc aagcatggtt
    99781 attcttttct ctatttcttc tattttctta ttttccaatt caatctctcc tttccaaaat
    99841 attctgtatt tagttttcaa agtactaacc taatattgaa gtttgataac tatattcttt
    99901 ttctatatct ggtaatatgt tatttgtttt caataaatca tataaaaaca atctaccttt
    99961 ttgagtccac ttagttgtca tttttacatc aggcattcca ttacttctag ttatgtctat
   100021 tgtttctgaa tgagtgtatc ccttcccttg atgttgttta tataaaagcc attgtccact
   100081 ttgtttatat tgaatccctc tttcatgaag tattttgttc atttcttttc cactcattcc
   100141 ataatccttt gctatttgag ttatagtgac tagacctttg tttttaagta tcatatctgt
   100201 ataatctgcc tttggtttta gttcttttat tacttggtct ttcatttttc cttctagttg
   100261 taatttctca tttacttcta cttgctctat aagatgttgt aatgcttctt tatatgtagt
   100321 tggtagttta ggttgttcat tttttaatac ttgctccatt tcttcaaatt tttttgtata
   100381 tattcctgta aatgccgtac cttttatgcc tgtcatttta tttgctatga agtcacaacc
   100441 tatctttgtt attgcatagc atggcttctc tcttttataa tcatctaaat aagtattttc
   100501 aataaaataa tcagatgggt acaaatctgt actcatgttt ttattggctt cttccatttg
   100561 gtttatataa tttcttatat cccttagtaa aatcttgtgt tctttttcaa ccattagagc
   100621 tatatctcta ctgtcagttg ttagtttatt attaactttt actattttta aatctttcat
   100681 atttaactcc tctcttttaa acaacatctt gttgtatttg tatttaaaaa aattttttct
   100741 attgatactc caaaacattt agataatttt attgctattg ctaaacttgg tactcttgtt
   100801 ccactttcaa tcattccata ataactagta gttataccta cttttaatgc aacatctttt
   100861 tgctttaaat tcctttctgt cctaagttcc tttagattgt tcaagctata tcctccttac
   100921 caacattttg ttgtttatac ttatataata cacaactata tgttgtttgt aaatagtttt
   100981 tttatttttt tatttcattt tacaactatt tgttgtatta tttaataaag gcaactttat
   101041 taagaggagt gattatatga gcattttatc agatagattg aaatttctta ggaaagaaaa
   101101 aggtgtaatg caaaaagaaa ttgccaacta tcttaacata actactagtg catatggatt
   101161 ttatgaacaa ggtaaaagaa cacctacccc agaaatgtta tctagtttag ctgaatattt
   101221 tggcactact gttgattatt taataggtag gtatgataat aaagcaagca atatttctag
   101281 caaaacttct tgtaataata cattatttca aaaaagacta aaagaactta gagctgaaaa
   101341 aaatatgact caagaagatg ttgcaaataa attaaactta accaaaagcg cttatggtta
   101401 ttatgaacaa ggaaagacag ttcctgatgc ttatatgtta tctagccttg ctgaaatatt
   101461 taatgtgact actgattatt tattaggtag gtctattgta aaaaatgata tagacactgt
   101521 agctgcacat agagttaatc ctcacaaaga tttaccagag gaagctcaag aacaactcaa
   101581 tgattatatt gaatttttaa taaataagta taaaaaataa attaaaagta ggtgataaat
   101641 tatatgaaat ctataatatc agatgtcata aaccgattta atgctacacc atttttattt
   101701 gttggttctg gtttaactag aagatactat aatcttccaa attgggagga tttactaaaa
   101761 gtctttgcag aaaaaattag taatgatgac tttatatata caagttataa aaataaagca
   101821 aaatcagaga acccaaaaat gggtataaat cctagaattg cagaacttat tgaaaatgat
   101881 tttaataaaa aatggttttc agattcttct atacgaagct tagactctaa gtatttagat
   101941 attgttaaat caggagtttc tccatttaag gcagaaattg ctatgtatat taaaaataat
   102001 tctaaaatag tagataaata tcaagatgaa gttgaaaaac ttagtaatat ctctaaaaaa
   102061 agtttatcag gatttattac aacaaattat gattgttttt tggaaactat cgttgataat
   102121 tatacaacat atataggtca agaaaattta gttttttcat ctatacaagg tattgcagaa
   102181 atttataaaa ttcatggatg tgtttcttct ccaaatagta ttgtaataaa tgaagctgat
   102241 tatatagact ttgacagcaa aagtgcctac ttggcagcta agctaatgac tatatttgtt
   102301 gagtttccta tcatatttat tggttactct gttacagatg taaatataaa aaaaatactt
   102361 aatgctatag ttaattgtct atctaatgaa aatgctaaaa aactcgagga acgtttcata
   102421 ttcattgagt atgaaaaaaa ctttaatgat atagaaatat caagtcatac aattgctttt
   102481 gataataaaa tgattactat gactaaaatt aaactggagg attttaattt actctatgag
   102541 gcattatctg aaaaaaaatc aaaattacca gttagaatat taagaatgtt caaacaagaa
   102601 ttttacgatt tcacaattac taacaaacct actgctaaaa ttcgggttgg tagtgttgat
   102661 gatacaagaa taaaagatga agatttagtt ttagcagttg gcaaggcaag tgattttggt
   102721 cttaaaggat taaaaggatt aagttttgat gaatggtatc gtgatatagt catgaatgac
   102781 ttagaatttt catcagatga attacttgaa tatgcttatc catctttaat taagcaatat
   102841 aataaattac cattaaataa acatcttttt aatagcacac ttgaatttcc agattatagg
   102901 acaattgcac tagaaagtga ctttgaaaat attattagca atagtattaa aaaaaataga
   102961 aataatactt acataaaaaa tagaagtgtt tatggtattt ggaatgatga acatcaatca
   103021 tttgaaaaag ctacaagact tattgctttt ttagaagaaa aagaaattga cactgaacag
   103081 ttagaaaatc tacttaagaa aatatttgag gaaaatccta atattttaga gtctgcaaaa
   103141 acaagcgaaa aaacaaattt aagaaggctt attagaatat atgactacat gaaatactct
   103201 aatatgcaaa agagtcttgg ttatagtgag taaaactcta cccaccgctc caaaactcct
   103261 ttaatcgctt taagcacaaa tacaaattta gtgctaacta tactaacaca catttgtaag
   103321 tgcttacata tattatatgt aattatatta taatcatata ctataatcca tattttgtct
   103381 acagctaaaa ttatccaatc ttttttggat aaaaaatgaa tatttagagc agttcacacc
   103441 tgctctttat atataacaaa aatcaaaaat acattctttt tataggggga tttcaatgaa
   103501 caaactagac gcacttttag acttagcaaa taatgaagag atagaaattt actacactga
   103561 caaaatagca gatgacataa aaggattgta tataaacaga caaggactaa agattatatc
   103621 attacttaat tcattaaaac aaaacaatgc taaactaata gaaatcttag cagaagaatt
   103681 aggacatcat tttaccagtg ttgggaacta tgtatcttca aaaaacagtt acaaaaataa
   103741 aatcttgata gacaaaactg aaaacaaagc attaaaatgg gcatgtgaat ttcttataac
   103801 agaagaagaa ataatacatg ttattaattc acacgctaca agtgtatacg aaatagctga
   103861 agaattacaa gttagcatca acttcttact aaaaagatta gaatttctat caaaaaagaa
   103921 aagcatgttg gacttaggaa ataatagatt tttagtatta actaatttgc caaatttcta
   103981 catatatgag gatatttttt aaactcattt attctacttt tatagatttt ttacttaata
   104041 aatatatatt tcaatattat tataataaac tacacataaa agctaaaaaa ttgtaagaat
   104101 attaagaaaa taattaagta aaaaccagat aaacaaaatt aagataatat tgtacataac
   104161 aaaagtatat aaagagcagt taatctgctc ttttatataa acaccaaaca aacatacatt
   104221 ctaaaaggga gggatactat tatgaaaggt ggagtaagaa aaagaagtaa caaatggtat
   104281 tactactttg acctaggcat agtagaagga aaaagaaaaa aagtagaaag agttggaggc
   104341 aatactaaaa aagaagccga aaaagcctta agagaagcac taaatgaata tgaaaattct
   104401 ggcatagtat ttgaagaaag caatatcagt ttatcagact acttagactt ttggtacaaa
   104461 gaatatgtct tacttaactg taaatacaac actcaagaaa gctaccgaat aaacatagaa
   104521 aaacatataa agccaaagct aggagcttac aaagtaaaag ctttaactcc tgcaatacta
   104581 caaaacttca taaataaaaa gtacaaagag gattactctc aaaatacatt acaagtatta
   104641 aaagccatat tacataggtc attaaaatca gcagtccatc cttacaaaca catacgagaa
   104701 aaccctatgc aatatgtaag cataccaaaa actaaatcta aaacagaaac taataaagtt
   104761 aaaactatta cagtagaaga atttaatcaa atactaaata tatttcctca agattcattt
   104821 caacgtatag ttttactaat tggatttcat actggtatgc gaagaggtga aattattgca
   104881 ctaaaatggg ataatataga ccttaataat aaaactatca cagtaagaca tactctgatt
   104941 aaaaagccaa atggaatgtt tgaattaggg caaccaaaaa cagaaagctc ttgcagaact
   105001 atatttacag gtgacacttt aataaaggca ttaaaagaac ataaattata tcaaaagaaa
   105061 atgaaattaa aatatggaga attttacttt gatagtgact gggtatgtac caaagaaaat
   105121 ggtcaacaag tgaatactca cactttagac actatagtaa gacaaattcg agtagcttta
   105181 aacaatgact tccattttca ttctttaaga catgcacatg ctactctatt attagaaaat
   105241 ggtgctaaca ttaaagacat acaaaaccgt ttgggtcata gccaactatc aactacaatg
   105301 gatacctatt cacatgtaac tgataaaatg aaaaatgaaa ctgtagatat atttgaaaaa
   105361 attacaaatt agagtttgcc acccaaaaat ataatacggt ggcaaatggg tggcaaaatc
   105421 taatttatct attttaaaag ctaaaattat caaatttata tagtcagcta tacgcatgta
   105481 atttcaaggc tttagagtat ataacaacca taactaatat aaggtattaa taataaatct
   105541 aatgaataaa actta
//
LOCUS       DAYASV010000002        88458 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYASV010000002 DAYASV010000000
VERSION     DAYASV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 88458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.002"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(157..756)
                     /locus_tag="ACGI68_000120"
     CDS             complement(157..756)
                     /locus_tag="ACGI68_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136584.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(957..1169)
                     /locus_tag="ACGI68_000121"
     CDS             complement(957..1169)
                     /locus_tag="ACGI68_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFH0136585.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(1242..2957)
                     /locus_tag="ACGI68_000122"
     CDS             complement(1242..2957)
                     /locus_tag="ACGI68_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0136586.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKVKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2957..3511)
                     /locus_tag="ACGI68_000123"
     CDS             complement(2957..3511)
                     /locus_tag="ACGI68_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFH0136587.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(3504..4106)
                     /gene="coaE"
                     /locus_tag="ACGI68_000124"
     CDS             complement(3504..4106)
                     /gene="coaE"
                     /locus_tag="ACGI68_000124"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFH0136588.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(4115..6763)
                     /gene="polA"
                     /locus_tag="ACGI68_000125"
     CDS             complement(4115..6763)
                     /gene="polA"
                     /locus_tag="ACGI68_000125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFH0136589.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            6915..7002
                     /locus_tag="ACGI68_000126"
     tRNA            6915..7002
                     /locus_tag="ACGI68_000126"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6949..6951,aa:Leu,seq:caa)
     gene            complement(7189..7632)
                     /locus_tag="ACGI68_000127"
     CDS             complement(7189..7632)
                     /locus_tag="ACGI68_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136590.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(7635..7832)
                     /locus_tag="ACGI68_000128"
     CDS             complement(7635..7832)
                     /locus_tag="ACGI68_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0136591.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(8024..8890)
                     /locus_tag="ACGI68_000129"
     CDS             complement(8024..8890)
                     /locus_tag="ACGI68_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0136592.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(9116..9985)
                     /locus_tag="ACGI68_000130"
     CDS             complement(9116..9985)
                     /locus_tag="ACGI68_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0136593.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(10491..10712)
                     /locus_tag="ACGI68_000131"
     CDS             complement(10491..10712)
                     /locus_tag="ACGI68_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136594.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(11213..12193)
                     /locus_tag="ACGI68_000132"
     CDS             complement(11213..12193)
                     /locus_tag="ACGI68_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136595.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(12357..13229)
                     /locus_tag="ACGI68_000133"
     CDS             complement(12357..13229)
                     /locus_tag="ACGI68_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0136596.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANSKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKYEK"
     gene            complement(13393..13944)
                     /locus_tag="ACGI68_000134"
     CDS             complement(13393..13944)
                     /locus_tag="ACGI68_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0136597.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(13964..14866)
                     /locus_tag="ACGI68_000135"
     CDS             complement(13964..14866)
                     /locus_tag="ACGI68_000135"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFH0136598.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(14881..17253)
                     /locus_tag="ACGI68_000136"
     CDS             complement(14881..17253)
                     /locus_tag="ACGI68_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HFH0136599.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(17474..18163)
                     /locus_tag="ACGI68_000137"
     CDS             complement(17474..18163)
                     /locus_tag="ACGI68_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0136600.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVSDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITNSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYVK
                     FIYNLFKSLTDTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(18404..19783)
                     /locus_tag="ACGI68_000138"
     CDS             complement(18404..19783)
                     /locus_tag="ACGI68_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0136601.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPYLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEVCLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENINKYIRIKGTITRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(19777..20490)
                     /gene="rgbR"
                     /locus_tag="ACGI68_000139"
     CDS             complement(19777..20490)
                     /gene="rgbR"
                     /locus_tag="ACGI68_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HFH0136602.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(20740..21291)
                     /locus_tag="ACGI68_000140"
     CDS             complement(20740..21291)
                     /locus_tag="ACGI68_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HFH0136603.1"
                     /translation="MEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGIFYF
                     HKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYKDIP
                     PKLYSDSDKTNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVLQII
                     LLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(21395..22198)
                     /gene="zupT"
                     /locus_tag="ACGI68_000141"
     CDS             complement(21395..22198)
                     /gene="zupT"
                     /locus_tag="ACGI68_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFH0136604.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(22349..23512)
                     /locus_tag="ACGI68_000142"
     CDS             complement(22349..23512)
                     /locus_tag="ACGI68_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0136605.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGDNPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAILEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(23572..24759)
                     /locus_tag="ACGI68_000143"
     CDS             complement(23572..24759)
                     /locus_tag="ACGI68_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136606.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(25122..26822)
                     /locus_tag="ACGI68_000144"
     CDS             complement(25122..26822)
                     /locus_tag="ACGI68_000144"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0136607.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(27065..27340)
                     /locus_tag="ACGI68_000145"
     CDS             complement(27065..27340)
                     /locus_tag="ACGI68_000145"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0136608.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(27459..28010)
                     /gene="mobB"
                     /locus_tag="ACGI68_000146"
     CDS             complement(27459..28010)
                     /gene="mobB"
                     /locus_tag="ACGI68_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HFH0136609.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLNDYKNISKY
                     IESCIKNEILSISNVEIKNILKE"
     gene            complement(28015..29196)
                     /locus_tag="ACGI68_000147"
     CDS             complement(28015..29196)
                     /locus_tag="ACGI68_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFH0136610.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVISDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKLSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(29407..30141)
                     /locus_tag="ACGI68_000148"
     CDS             complement(29407..30141)
                     /locus_tag="ACGI68_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136611.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            30503..31408
                     /locus_tag="ACGI68_000149"
     CDS             30503..31408
                     /locus_tag="ACGI68_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HFH0136612.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(31568..32410)
                     /locus_tag="ACGI68_000150"
     CDS             complement(31568..32410)
                     /locus_tag="ACGI68_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0136613.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(32394..33152)
                     /locus_tag="ACGI68_000151"
     CDS             complement(32394..33152)
                     /locus_tag="ACGI68_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0136614.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(33169..33639)
                     /locus_tag="ACGI68_000152"
     CDS             complement(33169..33639)
                     /locus_tag="ACGI68_000152"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0136615.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(33639..34382)
                     /locus_tag="ACGI68_000153"
     CDS             complement(33639..34382)
                     /locus_tag="ACGI68_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HFH0136616.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(34386..34799)
                     /locus_tag="ACGI68_000154"
     CDS             complement(34386..34799)
                     /locus_tag="ACGI68_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0136617.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(34825..35577)
                     /locus_tag="ACGI68_000155"
     CDS             complement(34825..35577)
                     /locus_tag="ACGI68_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0136618.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(35817..37211)
                     /locus_tag="ACGI68_000156"
     CDS             complement(35817..37211)
                     /locus_tag="ACGI68_000156"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0136619.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVIWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(37231..37440)
                     /locus_tag="ACGI68_000157"
     CDS             complement(37231..37440)
                     /locus_tag="ACGI68_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HFH0136620.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(37469..38914)
                     /locus_tag="ACGI68_000158"
     CDS             complement(37469..38914)
                     /locus_tag="ACGI68_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0136621.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            39024..39818
                     /locus_tag="ACGI68_000159"
     CDS             39024..39818
                     /locus_tag="ACGI68_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFH0136622.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            39869..41191
                     /locus_tag="ACGI68_000160"
     CDS             39869..41191
                     /locus_tag="ACGI68_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFH0136623.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(41292..42509)
                     /gene="cdeC"
                     /locus_tag="ACGI68_000161"
     CDS             complement(41292..42509)
                     /gene="cdeC"
                     /locus_tag="ACGI68_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HFH0136624.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(42692..42872)
                     /gene="ssrS"
                     /locus_tag="ACGI68_000162"
     ncRNA           complement(42692..42872)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGI68_000162"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(42987..43898)
                     /locus_tag="ACGI68_000163"
     CDS             complement(42987..43898)
                     /locus_tag="ACGI68_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136625.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDYDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDE
                     ELYNINEQESEYNYNEKNTKTRAKKKR"
     gene            44110..44508
                     /locus_tag="ACGI68_000164"
     CDS             44110..44508
                     /locus_tag="ACGI68_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136626.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(44631..45653)
                     /gene="ccpA"
                     /locus_tag="ACGI68_000165"
     CDS             complement(44631..45653)
                     /gene="ccpA"
                     /locus_tag="ACGI68_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HFH0136627.1"
                     /translation="MKMKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEV
                     IKETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANT
                     YSDKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYT
                     VSTSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKS
                     LIKYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVA
                     GFNDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRES
                     VTERKK"
     gene            complement(45900..46100)
                     /locus_tag="ACGI68_000166"
     CDS             complement(45900..46100)
                     /locus_tag="ACGI68_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136628.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(46134..46313)
                     /locus_tag="ACGI68_000167"
     CDS             complement(46134..46313)
                     /locus_tag="ACGI68_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136629.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(46495..46701)
                     /locus_tag="ACGI68_000168"
     CDS             complement(46495..46701)
                     /locus_tag="ACGI68_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136630.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(46749..47174)
                     /locus_tag="ACGI68_000169"
     CDS             complement(46749..47174)
                     /locus_tag="ACGI68_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136631.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            47346..47573
                     /gene="acpP"
                     /locus_tag="ACGI68_000170"
     CDS             47346..47573
                     /gene="acpP"
                     /locus_tag="ACGI68_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0136632.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(47717..48721)
                     /locus_tag="ACGI68_000171"
     CDS             complement(47717..48721)
                     /locus_tag="ACGI68_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFH0136633.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(48735..49637)
                     /locus_tag="ACGI68_000172"
     CDS             complement(48735..49637)
                     /locus_tag="ACGI68_000172"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0136634.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEELINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(49832..51007)
                     /locus_tag="ACGI68_000173"
     CDS             complement(49832..51007)
                     /locus_tag="ACGI68_000173"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFH0136635.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(51094..51939)
                     /locus_tag="ACGI68_000174"
     CDS             complement(51094..51939)
                     /locus_tag="ACGI68_000174"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HFH0136636.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(52037..52834)
                     /locus_tag="ACGI68_000175"
     CDS             complement(52037..52834)
                     /locus_tag="ACGI68_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFH0136637.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(52853..53863)
                     /locus_tag="ACGI68_000176"
     CDS             complement(52853..53863)
                     /locus_tag="ACGI68_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0136638.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(53882..54664)
                     /locus_tag="ACGI68_000177"
     CDS             complement(53882..54664)
                     /locus_tag="ACGI68_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFH0136639.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(54723..55859)
                     /locus_tag="ACGI68_000178"
     CDS             complement(54723..55859)
                     /locus_tag="ACGI68_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009272751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFH0136640.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(56568..57035)
                     /locus_tag="ACGI68_000179"
     CDS             complement(56568..57035)
                     /locus_tag="ACGI68_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFH0136641.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(57114..57656)
                     /locus_tag="ACGI68_000180"
     CDS             complement(57114..57656)
                     /locus_tag="ACGI68_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136642.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(57708..58394)
                     /locus_tag="ACGI68_000181"
     CDS             complement(57708..58394)
                     /locus_tag="ACGI68_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0136643.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(58394..59254)
                     /locus_tag="ACGI68_000182"
     CDS             complement(58394..59254)
                     /locus_tag="ACGI68_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0136644.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            59389..60189
                     /locus_tag="ACGI68_000183"
     CDS             59389..60189
                     /locus_tag="ACGI68_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HFH0136645.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            60214..60783
                     /gene="yfcE"
                     /locus_tag="ACGI68_000184"
     CDS             60214..60783
                     /gene="yfcE"
                     /locus_tag="ACGI68_000184"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFH0136646.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(61118..62170)
                     /locus_tag="ACGI68_000185"
     CDS             complement(61118..62170)
                     /locus_tag="ACGI68_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HFH0136647.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADTISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(62377..63603)
                     /gene="pepT"
                     /locus_tag="ACGI68_000186"
     CDS             complement(62377..63603)
                     /gene="pepT"
                     /locus_tag="ACGI68_000186"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFH0136648.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(63693..64745)
                     /gene="ytvI"
                     /locus_tag="ACGI68_000187"
     CDS             complement(63693..64745)
                     /gene="ytvI"
                     /locus_tag="ACGI68_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFH0136649.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFRINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(64894..66147)
                     /locus_tag="ACGI68_000188"
     CDS             complement(64894..66147)
                     /locus_tag="ACGI68_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFH0136650.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(66278..69805)
                     /locus_tag="ACGI68_000189"
     CDS             complement(66278..69805)
                     /locus_tag="ACGI68_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0136651.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKDNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(69792..71003)
                     /locus_tag="ACGI68_000190"
     CDS             complement(69792..71003)
                     /locus_tag="ACGI68_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFH0136652.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(71049..74876)
                     /gene="addA"
                     /locus_tag="ACGI68_000191"
     CDS             complement(71049..74876)
                     /gene="addA"
                     /locus_tag="ACGI68_000191"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFH0136653.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGCRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRTNNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKNDILVEKKSDEEKESIEEILEKIDVNNPDSDY
                     YGEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(74876..78343)
                     /gene="addB"
                     /locus_tag="ACGI68_000192"
     CDS             complement(74876..78343)
                     /gene="addB"
                     /locus_tag="ACGI68_000192"
                     /EC_number="3.1.-.-"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HFH0136654.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNPTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(78333..79163)
                     /locus_tag="ACGI68_000193"
     CDS             complement(78333..79163)
                     /locus_tag="ACGI68_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFH0136655.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(79272..81491)
                     /locus_tag="ACGI68_000194"
     CDS             complement(79272..81491)
                     /locus_tag="ACGI68_000194"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0136656.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDNLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     KECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            complement(81484..83397)
                     /locus_tag="ACGI68_000195"
     CDS             complement(81484..83397)
                     /locus_tag="ACGI68_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136657.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERSLRLNYSEELKITTYISFVKKELVKYWPLIIEKCDEISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYREILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFIEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            complement(83639..85651)
                     /locus_tag="ACGI68_000196"
     CDS             complement(83639..85651)
                     /locus_tag="ACGI68_000196"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0136658.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLNTTLNYNQSTGAYEGTINYNQDPE
                     YLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTVNASQTRLNGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKDSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKDNLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSAPGNSSGGMIEKD
                     YNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPDLFTSIHYNGSTNK
                     QGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVL
                     RSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSLK"
     gene            complement(86156..88174)
                     /locus_tag="ACGI68_000197"
     CDS             complement(86156..88174)
                     /locus_tag="ACGI68_000197"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0136659.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIR
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT        27902 a        15655 c         8992 g        35909 t
ORIGIN      
        1 agttaaaaat ttgaatgatg atgaccaatt accaaatcat ggtaaattat ttaaaagaat
       61 atgtcaaaaa aagaataagc tctttcaaat gcataaaaaa tttataccca agttatactt
      121 aatcatgcaa attggcagaa cttattcttt aaaactctaa cttttaagtt tttctttaat
      181 aatataattt attacttttt ttctaatatc ttcagcatta taatcatctt ctacttctat
      241 atccaaaagt tttgctattg acctcatatc ttctctagtc tttgacctat agcttgatat
      301 taattcatct tttattttct ctggttctaa aagatattct tcatctcctc ttttgaaaac
      361 aacgtatttt tgagcatatg aacttctatt gtagtatata tatcttgata tttttcttaa
      421 aatttgatta tatgaaagtc gcttgttaat atcaagtttt aagtactctg aaaaagcaaa
      481 tagctctttt tttgatttaa ataatgcttc tttctttata gttcttgata ttgaagttac
      541 actttcttga tatgcacact tttttaacat ttttaaatat actctattaa gaccaatact
      601 ttgattgaat ttttttatat tattattaat aatcttatcc aagttacctt caaagtattt
      661 ttttgctcct ttttgtgata cataactctc aagttctttg tgggccatat caagtatcat
      721 tttttttaaa tcctctttat atcccctaga ttccatgata ctccctccct tagctattta
      781 tagtatattc aaaaagctca agaaatgtga gtcattacaa aattttttta ctttttatgt
      841 atatattaat ttaaaagggg agataatagt aatgtaaaat caaacatact caatctcccc
      901 catttttaga cctctattac tcccccaata gaggtctttt tttagtttat caatttctat
      961 tcaattccct ttagttcaaa tcctaattct gctatcactt cttttaattt ttcaatagat
     1021 acatcatctt tcatatctac agatgcacac ttattttcta aatctacatc taaaacttca
     1081 ataccatcta tatcttctgt taatgcagtt ttaagatggt ttacacaatg accacaactc
     1141 ataccttcta ctaatagttt cttcttcatt taattacctc cttataccct ataagggtat
     1201 tctatcatat agtttatact ttttaaatag aaattaatca tttaatttga ttcattcgat
     1261 tttatatcta aattagatat tccctcatat acatcaaaat catttggagt aattttacct
     1321 tttaatctac tattacttac aatatagttg tttctcacag ctaaacttat aaatgttgca
     1381 ttatctttta tatatgtctg tacatctttg tatgcttttt ttgtttcaat ttcagacgta
     1441 gaacttgtaa gtgcagacat atatccattt aacttatcat cagtatatcc actactcgct
     1501 ataatactac ttgcatctgg tataattgat agtttccatc caactaatgc taaatcataa
     1561 tttttagaat taagagcatt ttccatatcc ttatctgaga gctgatttac tgtactattt
     1621 attcctatag cttttaagtt ttcatttatt atgtttgcag tttttattct ctcactatta
     1681 tctctgttta ctattatctt taagttcatt tcagaaagac ttttctttac ttcctctttt
     1741 tctctatttt ttttctcttc agcttctttt ttgattctat cttcttctgc ttttttaact
     1801 tttgcttctt cttctggcgt aagcttcttt gagttctttt tagctgattt tttctgattt
     1861 tttactttat tgtcattttg atttactgag tttacatttg catattctac cttagcaaga
     1921 cactctatcg ccttatcttt attaaactct aaatccttca tttcactatt atagtatttt
     1981 gattttgaat taagtggaaa attaactgga gtagcatcat ccatataacc ttcactaata
     2041 attctatctt tatctataga gtgtgcaatt gcttttctaa aattaacatc tttgaaaaaa
     2101 ggattattat aattaaataa tacacactca taatctcttc cttgatattt tgttatatta
     2161 aactcttttt cttgaaactt acttaaatca tttaatgtaa cattggttat atcactatca
     2221 agagccataa ccatagatgt ccttgcatct tcattaggca ccatacctac ttttatgttc
     2281 ttcatggttt ttggaacttc ttcatagtaa tctttgttta cagataaaac cattccttct
     2341 ctttctgtat atgattctat tttatatttc ccatttccta tcatattatt attcttgtca
     2401 tttacatctt ttgaatctaa atgattttga gatacaattg gaaaaattaa actatcaatt
     2461 gaaaaagcat atctagcttt aaattttatt gtaaaatctt tatctgattt aatattcaca
     2521 gaagctatat ttttagtaaa ttcattatat ggactatctg cacttttttg aattaaacca
     2581 atagtatagt ttacatcatt tgaagtaaca ttttttccat catgccactt tgcatctttt
     2641 agctttatat ctatactcat tccatcctga gctatattat actccttaac taattgagga
     2701 attacattgt agttctcatc tatagtaaat aaactatcat aaactaaatt cattatataa
     2761 ccaacagact tatcagtatt agttatagga tttatagttt ttggagttac cattgttaag
     2821 tttatatact tagcatctat agaactttga ctttgtaaac ttgaatcact actactttgt
     2881 tttgttttag tattactaca accagctacc ataagagtta tagctaaaac gactgttaag
     2941 acttttattc tcttcattag ttttttcctt tcttgccata tatcttatta taatgttcgt
     3001 aattattttt tactttttct acatattttg aagtttgctt aaaaggtata tcatgtagat
     3061 tttcgccatc tttactatat tcattatttt ctaaccattt tttcacatta cctgaaccac
     3121 cattatatgc agctatcact aaatctaatt taccaaattc tttgtataac ttacttaaat
     3181 accaacagcc aagtcttata ttagtctctg ggtcgaaaat atccacattt tttaaattta
     3241 gttcctctgc tccccaatct ctagttatgt ctaatatttg cattaatcct tttgcttcct
     3301 tttttgaaac agcagaacta ttaaacttac tttcggcttt aataacactg taaactatat
     3361 tttcatctaa attaaattct ttcgaatact tttctacata ctctgaatat tttttaggat
     3421 ataaaaattt atgtattact ttgctttcca ttaatagtgc cccaaataag attataaaaa
     3481 tagatagaat caatactttc ttactattca cgccaattct ccttcatgta ctcaataaat
     3541 ttatgtgctt tactttctaa ttcagttatt gttcccgaat tatctattat ataatcacca
     3601 tatttggatt tttcttcctg actcatctgt gaatttatcc tactaagtgc ttcctgctca
     3661 ctgcaattat ctctcttttg tattctacta atttgtactt cttgtttaca agtaactact
     3721 aatagcttgt caaccatatc taaaaaacca ctttctacca atattgccgc atctaacact
     3781 actatatttt cgcctttttt tctcaatttt tctatttcac ttattatttt ttctcttatt
     3841 cgtggatgag tcaagttatt taattcctta agtttttctt catcactaaa aactattttc
     3901 ccaagagctt ttctatttag agttccatca tcatttttta tactttgccc aaaatggaca
     3961 aatacttttt ccaataacaa cttatcctca aatatcttcc tggatataat atcagcatcc
     4021 actataggaa tgttaagatt tctaaatata tttgataggc tgctttttcc acagcctatt
     4081 cctcctgtaa gtcctaatat caacataaaa tcacctactt tgtctcatac caagaactac
     4141 ctacatttaa atctacatct aagtgaacat ccatatttac agcattttcc atttcttctt
     4201 ttactatttt cttaactata tctatttcat catcaattgc ttcaactatc aactcgtcgt
     4261 gaacttgtag tataagtttt gatttcaaat tgttctcttc caatttttta tgaacattta
     4321 tcatagcaat ttttattata tctgctgcac ttccttgtat tggtgcattc attgcaaatc
     4381 tctttcctct atttctttcc ataaaattgc ttgactttat ttctggtata tatcttcttc
     4441 tgtttaaaat tgttacagca tatccatcct cactagcctt ttctactgtt gtgtccataa
     4501 actcttttat tttatggtat ttagcaaaat aactttcgat atattctttc gcctttggaa
     4561 caggtatatt taaatcatca gctagaccaa agtcactttt accatatata ataccaaagt
     4621 ttacagcttt agctgcacct ctcaattcac tagtaacatc ttccatagga acattaaaaa
     4681 cttgtgaagc tgttttggta tgaatatctt catgatgttt aaatgcttct atcatatttt
     4741 catcctgact catatgtgca agaactctta gctctacttg tgagtaatcg gcatcaacca
     4801 acttacagtt atcatctgca ataaacactt ttcttatttt tctacccatt tccattttta
     4861 taggtatatt ttgaagattt ggttctgtag atgaaattct tcctgtagtc gttatagttt
     4921 gattaaatga tgaatgtatt ctattacttt ctggatttat tatatttaaa agtccttcaa
     4981 cgtaagtaga atttaacttt actatttgtc tatactcagt tatcttatct attattggat
     5041 gcttatctct taatttttct aaaacatctg cattagttga atatccagtt ttagtttttt
     5101 taattattgg aagctctaat ttttcaaata atataacacc caattgtttt ggtgaattta
     5161 tattgaatgg ctctcctgat aattcataaa tttccttttc taatgtagct attatttctt
     5221 taaattcaac actcaattca tccaatatac ttttgtctac ttttatacct tcatactcca
     5281 tatgacctaa gacttctact aaaggcatct ctacatgata aaaaagacca tccatactca
     5341 tatccattag acttctttcc atcataggaa ctgtttcttt aactgtgcat attatttgac
     5401 ccatatactc tgctaaatcc tcaaattcta aatcctcata gtttttagat ttaacaccct
     5461 ttcctaataa ttcttctttt gatttaacct tttttgataa atacttcata gctattgcac
     5521 tacaatcata tgaggtagat gaagttgaat ctattaagta ctcagctatt gttatatcaa
     5581 aatacatatt ttctaagttt atattatatg gttttaaaga tatataatca tcttttaaat
     5641 tatatccata ctttttaatt tcattatttg aaaatatact atctaacttt gacacttcat
     5701 ttgaatctac ataatatatt ttttctccat ctacacttat aaaaatatat attatatttt
     5761 tttcaagtat attaccttgt cctctaacag tttttaaaat aacttgtcct tttttgttta
     5821 cttcatctat aaatttttct atatctttta attttaatat ttctattttt tcttctacgt
     5881 gttctgtgtc atctgaatcg accaaatcaa ctaatcttga taagagactt gtaaatccaa
     5941 agtatctaaa tttatctaaa agttcatctt tatcataatc gccaaaaatc atactatcta
     6001 aatccacttc tattggcaca tctcttataa ttgttgcaag tcttttactt tgaatagcta
     6061 tttctttgtt ttcctctatt ttctttttta cactaccttt aagcttatct gtattctcta
     6121 ttagattctc tatactagaa aactctttta taagctttat acctgttttt tcacctattc
     6181 caggtactcc aggtatatta tcagacttgt ctcccataag accctttaaa tcgataaatt
     6241 gtgttggagt catctcgtat ttttcaatta cagaattaaa gttgtattct tcaacttctc
     6301 caactccttt ttttgttata agagtagttg tattgtctga agcaagttga atagcatctt
     6361 tgtctccagt tactatgtat accttatatc cattttcttc tgctttttta gaaacagtac
     6421 ctataatatc atctgcctca tagccatcta tttccaatct atttattcta aatttatcta
     6481 aaaggtcttt taatggctgt aattgttctg ccagttcatc tggcattccc tttcttccag
     6541 ccttatactc tttatattct aaatgtctaa atgtaggtgc ttttctgtca aatgctacac
     6601 ttatatgtgt tggtttatat atatctatca ttttaaatag cattgttgta aatccatata
     6661 ttgcattggt ctttagccct tctttattat tcatttctgg aagagcataa aaagctctat
     6721 ttattattga atttccatct attataatta attttttttc caaattatat cactcctaaa
     6781 aattatctat ttttgtaaca tatatattat ataccaatat actgttattt aatattccca
     6841 taataataat agtatattaa aattaaatat gttgcaaaca atatattttt ttgctataat
     6901 aatatacatg acatgcccga gtggtggaat tggtagacgc agtggactca aaatccaccg
     6961 gactaatact ccgtgccggt tcgagtccgg cctcgggcac cattaaaaac tcctaaagct
     7021 atttattcaa tagattagga gtttttttat ttttgattta aattttaagt tattttttga
     7081 aattactgtg tctttttaaa atttatataa ttatataaat ttatcttcta gcattaattt
     7141 aaaaactaat ttatttttat ataattttct attttatatc ataacttatt acaacttttt
     7201 atctaattta ttgtatacaa caacactaca aataaattga aataaaataa gcaatccaag
     7261 aacaactaaa acccacttgc ttattaccat tacagaaaat acactcaata tagaaacaat
     7321 aaatataaga attacaagaa cataggacat gatttgatta gtgatacgtc cagctttatc
     7381 acgtaacata attcgtcttt catcattcat ctctatcctt tgatttttta tcttttcttc
     7441 ataatctact gctctttcag gtttactcca atacatatat tgaagaatca tacatacacc
     7501 tggacctaaa gcagctccag ataatcccca aataaaagcc tcaaatgaaa actcagtaaa
     7561 tgtagccaat aaaacaagta aaattccaca aataaaatat ataattccaa catataacat
     7621 attctttttc atttttactc ctcctcataa ataaatattt cttcaatact catatcaaag
     7681 tatctagcta tcttaaaggc taagatgata gaaggattat acctaccatt ttctaatgaa
     7741 cctatagttt gtcttgatac ttgcaaaata gaagcaagtt cttcttgttt tatgcctctt
     7801 tcttttctta tttcttctaa tctatttttc acatctatcc ccctttaata tctcaactac
     7861 ggaaagtcta ctttccataa ttatacaact atattccatt tatgtaaagc ttactttcca
     7921 tatttacaaa aaaataacag actattttca gcctgtaatt ttagaatttc atgttgttta
     7981 ttaattttaa tgttatttat taattccata ttatttatta attttaactt ggtttctgaa
     8041 ttggaatcca aacttctgta gtataatcag gagcattgat atcgccattg ggataaagtt
     8101 caatatctgg taaattagca tacacatacc cagaacttgg taaccactca gtaataattc
     8161 ttttttgcaa ttcctgtatt gccagagcat ttggcattgg agaaacacat tcaaatactg
     8221 cccatgtgcc tgatggtatt aagtattcat gagtatcttc taatacaggc ttatctgttg
     8281 gagcagcaat ataataatct aaacccttac tatttgtaca cacactaact ccaagcaatc
     8341 cacaaggttc attatttatt aactctgaaa gtttatcaat tgttccattt tttatgcatt
     8401 tagcccacaa cttaggaact ttctcaaagc actcttctac aatcatgttc atactttctt
     8461 ttataccaac aatacgaaac cctttctttt ctacaatacg atattgcatt tctatatttc
     8521 cttttacata aatgcttaat gtcatcttag gatatgcttt taaaacaaca ccctttgctc
     8581 tagctgcaga tggagaaatg ttatgtatat tttgaaaagc tctattaaaa gatgttggtg
     8641 aatcgtaacc atacttctca gatagttcta aaattttgat gttactgttc tgaagttcaa
     8701 atgcagcctt agtcatacgt cttcgtctaa tatattcaga taatggtact tctgcaatat
     8761 atgaaaacat tctttgaaaa tgaaaggttg aacaacatgc aatttttgct gcttctgcat
     8821 aatccactcc actttcgaga ttttcttcaa tataagtaat agcttgattt aatttattta
     8881 tccattccat ttgatattac ctccaagcaa attatacagt ttaataaaat aatatacctc
     8941 tctattcatg cacaaaattg taagcattta aatttataag catttttcaa tcaatacaat
     9001 aaatataaaa attgtattat acaataaaaa agttcaataa cattatatta taaaatgtta
     9061 ttgaactttt tataaaaatt aatttaagtg tagttaaatt aagtattaca ctatcttata
     9121 tattacaata tcctactatc ttatagtctt ctggcacttt ataatctttg tctggagtac
     9181 ctaaattcca ttttaaatca aacatagttg cacttattat tgttgcaaaa taaagcataa
     9241 ctataccaat gtctattttt tcttcatata aattagtatg accatctttt ctaacagcta
     9301 aaactacagt gcctccatca actataaatc tccatggttg tctatttaat gtagatggag
     9361 ctaatcttgc atagtgaaat gcatcaagaa gtgctctttc ttctaagatt tctatacttg
     9421 caacatttcc ccattcgttc atataaacaa catcttctac acctagtcta gtagacccct
     9481 tatctgttac aacttgcata tcagattttg aataattttg cccagttttt gtagcattta
     9541 atactttagc attacttaca ttttctccat atccaatagc tataattcct gttacctctt
     9601 tatctgaatt aattccaagt ttttctttta ccttttttcc atcttcaaaa gtaacccaac
     9661 aagaaccaat tccaagttct gttactttaa agattatact ttctcctatg taccctgaat
     9721 tttcaatgta attatctttt atgtcagata atattataat ataacttgga gaatctatca
     9781 tatttccttt gtaccctgct actttatcta aattattaaa aacttcactt ctattcataa
     9841 ttcttacatc aacatctatt tctggaacta gtttcttaca agattctgag tatttcttaa
     9901 tttcactaag atattcttct ttaatatctg tgttcttata ttctctaaca gactttctat
     9961 ttaatatgat tgttttataa tccataattt tatcctcccc aaactagtat tgtcttaatt
    10021 atatacccat taatatatta ttttactcat ttatttaata aataaagatt acaattttgt
    10081 aattattatt tattaataat attccacttt tacattaata atgaccagtt gtgcaaaatc
    10141 cattgccaac caccaaataa aaacatatta taaaggtaat ataaaataac aaacaaatct
    10201 taacaaaaaa ttaaactttt tttacacgaa agaaggcttc ccgttgcctt ctttattttt
    10261 ataaaaaaat aagctgtttc taaaatgatt tcacattttt gaaacagcca agttattcta
    10321 taatgacaac tataatttga ggatgtgagg gagagttgtc attataaatt tagggtaatt
    10381 atattatgtt ttaagctagt tctttctata tttatataat acattttata tatgaatttt
    10441 atatgagatt ggtataaatg atatatgaaa ttacatatgt ttatattata ttaaatttta
    10501 ttattacatt ttccacaaca atactcatat gtctccacat tatttggaat atttacaaat
    10561 acaaattctt tacagttctc acaatacttt aatatagata tagttccaca agtgtaacat
    10621 tcaaatatta cacaatcctc attttcctct actatttcaa actttttact atgttcacca
    10681 cactctgggc acttgtgatt atttacattc ataaatactc ctccttatta gtatagttac
    10741 atgtcaaaaa cttaattaat gtattctata ttacttatta tatattctat aataaatcat
    10801 attctaatat aataaaacaa attaaataaa aaaaaaccat aaattttaaa tttatggatt
    10861 aattcttgta atgataactc tatttgagga tgtgagggag ggttatcatt acaatagggt
    10921 attatattat ggtttttaag ctagtctttc tacacctcat atgatacata tgctatatga
    10981 attttatatg atttttacat aaatatttta tgaaatttaa attttttagt cattttgaaa
    11041 tttgtaaaaa gttctctaaa aatccacagc atcataaaca gccaaaattt aagtagaaca
    11101 tcataaaaaa atttgtccaa taattttaac aagtaatctc acctcattat aattattgac
    11161 acaaacttaa ataaacaatc tataaatctt ctataaatct taaactttcc ccctattgat
    11221 acacaatttc tagtttgtca attttatttt gcatttctaa gaaaatttct tcaacagaat
    11281 tgttaaatat attgcctaaa tagattcttt tatctttttg aaatgaataa gattttacac
    11341 taccatcaga agaaatatat aaatgactcc actctgctcc atattttgaa aacctctcct
    11401 ttgtatcttt aaacctcata tcttctattt ttattgcttt ttctctgtct cctcttacta
    11461 cagtgtgacc cctttttatt aaactggtta tttttatatc ttcttttaat ttaatacgtg
    11521 tatcatcatt ttttgaatat actttccata tcactttatg acgataatta tctaattcct
    11581 ttattaagct tctatcaata gtaaaactat ctttagttac cacaattaaa tcaaaatggt
    11641 ctaacatata ttgaaatatt tctctcatac ttttattttt taaaatatcc tcaccaataa
    11701 cctctataaa tctacagcct ttctcataca tgcagttcaa taaacttgtg atttcagaaa
    11761 aataatcatt tgtattatta tctccatcta tttctatagt aaagtacaaa ggagtaatgt
    11821 atgatttatt tcctgttatc tcaaatacac ttgatagtgt ctgtatatca tcttcaaacg
    11881 ttaagtatac attctcataa aacataaaaa attccataac tttttctttc atattatgga
    11941 tatttctata tctattggat aatatattta ttatatcgtc agtagtattt ttcccatcac
    12001 attcagccag tataacaatt tgcttccaac ttaagctatc cacatttccg atactttttt
    12061 tatatccatc ttcttcatca taaataggac caattataat gttttctttt ggccacagat
    12121 gaaccctagt tccatctttt aatttcggat atttattttt tacagactca taaaatctac
    12181 ttgtatcacc catgattctt tactaccccc catagtttaa atattctatg ctaaaaatag
    12241 cactttattt ttattaattc aacaattatt caaaattggt cattatatat gtttaaatgg
    12301 atttaacatc aaccttattt atatgttatt gatgtttata tatgttatat aaaatcttat
    12361 ttctcatact tcaatgtaat cttaactatc tctggtttac aattaagcct aattaaaact
    12421 tttgagcctc ctaagccttt atttaaaatc atagtgctat tatctttttc atattttccc
    12481 cagtcatact taggaaagaa ctctctattt ggagataata cacctccaac aaaaggtatt
    12541 cttattatac ctccatgaac atgtcctgct aaaaccaaat ctgctcccca ttttgcatat
    12601 ttttcaaaga aaaatggtgt atgtgctaaa agaatgttat attcattttc attaacttta
    12661 cctagattgt cttctacaaa attgtcactt agttctaatt ttttgctatt agcaaataaa
    12721 tatggatagc attccaatgg tataattaaa ccatataaat taagatggct attacctcta
    12781 tatattctta cagattcatt ttccatattt attattttct ttgaatatag ttccttaaaa
    12841 taatccttat atagatgttt atactttttt attaaagatt tttgttcatg atttccaatt
    12901 atatgatata cttcatattt ttcaaccaaa ttatctatta aatctaatgc aacttgaaaa
    12961 tttttatttt ctccatctac taaatctcct gttataaata taacatctgg gtttaatgat
    13021 tcaattttat ctatcaatac cttattattt ttatcaaaca ccttattatg taaatcactc
    13081 acttgaacta ttttaaagtt atcaaattcc tttggaattt ttttgttgaa aatagtatat
    13141 tctgtcgttt ctatatgctg agtttcatac ttatacagaa tatacaataa tattaatact
    13201 aatactgtca ttgaaacctt tataatcata ttctcttcct ctttattcta ttttgttaat
    13261 attattataa tgcttttatg ttttttttaa aacaataaca tcatcacata catataaatc
    13321 ttttcgacat tataaaaata agttatcctt ttctaaaatt ttcatttatt ttagaaaagg
    13381 ataactataa aattatctat aaacctctat attgtgaaat atagtttttc ctttatttaa
    13441 atttttatat atatgctcta aagttccatc aaacctaata gaatgtccag actctaaaat
    13501 ataagttttc ccatccacat taaggtcaag tttaccttct attacgagta catactcttc
    13561 taaaacatga ctgtgtgtag atgaaacatg agtacagttt tcctcaagct caattataaa
    13621 aagttcaaac cttctttgag ggctaaaagg gtatattgga tataacttca tgttcccttt
    13681 ttgctcaaca ataggctcta cttcttcttt ctttataacc tcataggtat ttgtattttc
    13741 atttaatagt gtagtaaaat ttattttaaa tccagatgaa attttccata aagtagatac
    13801 tgtcggactt gattcacctc tctcaatctg tccaagcatt gctttactga cacctgtcat
    13861 ctttgaagct ttgtctaaac ttatgcccat ttcttgtctc aataacctta aattttctcc
    13921 cacattagga aatttattat ccatgttaaa atcctcctga tagctatttg tattctatga
    13981 tttttactcc tgtaagttct actatctctt ttaattttaa catatgattg ttacaaattt
    14041 catcaaattc atatatataa tcttcaagcc ctaatgcctt atatttatcg attccaaatt
    14101 tatgatatgg aagtaactcc atcttgcctc ctggaacatt ttgtttgaca aataatgcag
    14161 tatttcttat attttcttca ctatcattta ctccataaat aattggcact cttataacca
    14221 tagatttatt caactttgaa agcctacata tattatctaa tataatttta ttacttactc
    14281 ctgtatattc tttatgaata ttatcatcca tagattttat atctacaaat atgtggtcta
    14341 ttttctcaaa tacatcattt accttattcc aatcaaaata tccacaagtt tcaattgcaa
    14401 tatttatgcc tttatcataa aaaatatcaa ctaattctct taaaaaatca atctgaagtg
    14461 taggttcacc tccagaaaaa gttatccctc cattagattc aaagaaaaat ataaaatctt
    14521 tttcaacttc ctctactatt tcttcaacac tcatttcttc ggtcattata caaatggcat
    14581 ctttaagaca taccttgata cactctccac ataaatcaca tttattattt atttgcgctc
    14641 tatcaaatag tgaacttata ttttgaggac aaacatctat acatctatta caggatacac
    14701 atttatcttt cataacacct aattttacta tgttactcca ggaatctgga ttagaacacc
    14761 acttacattt taatttacat ccttctaaaa atatagtagt ccttattcca tctccatcat
    14821 ttactgaaaa atgttgtact tttacaactc tacctttttt actcataatc tacctcataa
    14881 ttaaaaattg ctatgtgaat ttctagctat tatagcatct tgcatttctc ttgataaatt
    14941 aacaaattga gtactatatc cagcaactct cactaataaa tctttatatt cttctggatt
    15001 ttcttgagct tttaatagag tatcatttga tatcgtatta aattgtacat ggaatcctcc
    15061 taaagcaaaa tatgatttaa tcatagatgt tagatttcta agtcctcttt cagtttccac
    15121 caaatcttga ttcagtctta aatttagaag tgttccacca gtatgaacat catgatttat
    15181 ttttgatgca gatttcattg ctgctagtgg acttgttgta tcagtaccaa caaatggaga
    15241 aactccctct gtcaaaggtt ttgtcgcttt tcttccacaa ggagttgcgc ctacgatttt
    15301 tccagttggt acatagtttg atatacccat aaatgcacta ttaaatttag agccaaaaat
    15361 atccttgtat tttctagttt cagtataata aaaatcagaa acttcaattg ctaatgaatc
    15421 tacataatca ttatcatttc catacttagg aacatccaac gcaagttttc ttaattcttc
    15481 ataaccttcc caatcattat ttagtgcttt tgttaattct tctaacgtag tcaccttatc
    15541 ctcaaatact aattttttta tagctgccaa tgaatttgaa actacaccta accctatacc
    15601 agttaataca ggacctatat tatattttgc tcctccttta cttaaatcaa ctcccttatc
    15661 caaacatcca tctacacaag tagataaaaa tggtcttggt accatttctt tatgaatttg
    15721 ttgagcaaca gtagttccta taacagaact ttttacaaga tatgctaatt gctttaaaaa
    15781 ctcatcctta agttcttcaa aagaagtaaa ttctgtgata ttttttgtat ctaaccccat
    15841 tttttctcca gttaatctac tcttaccttc attcatagca tattctaaag ctgctgcaaa
    15901 attgatattt acggctgaag tccactgtcc tgtttttcta aaatgaggaa ctacacaacc
    15961 acagttactc caatctctag catcttctgc atcatatcca tcttgtaata acatttgtgc
    16021 ccctgcacta tcgctgtgta tagcagggaa accagttcct tgttttacca atttagcaac
    16081 tgccattacg aaattgtctg gtgctccttg actcactcta acacttaatc ctggttggtg
    16141 agtttttaca ctttctgtag cctttaaaca catatatgag atatcattcg ttccatcagt
    16201 accatttctc tttttaccac caacagtaag attttggaat tgattatatc ctgcaaagta
    16261 atttgctgta tttgctgata tagtccatac ccattctgat aacttaatcc aaagtgcttc
    16321 tatcaattct tgagcttctg attcagttat tttaccttct ctagcatcat tttcataata
    16381 tggatacata tattgatcaa atctacctaa atttaatgcc aatggatttt ctgatagaat
    16441 accacctaat tgaacaaacc atacaaattg tattgcttca taaaaattag ttggagggtt
    16501 tgctggtaca ttcatacata tctctgaaat ttttataagt tctaactttc tcttttcatc
    16561 tgtttcttgc ttactcatct cttttgcaag atttgaatat ctttgtgcca atgtcataat
    16621 accctgtgca actatagaaa cagatttgta aaaatctatc ttttcaatat tttctgaaac
    16681 tgatatatct aattctttaa tttttttatc tgcatcttcc ttaatccctt tatatccttt
    16741 tacaaacaat atatcttgat aatctggtgt tacttcacca actgcctgtc tccacttaga
    16801 atcattatct attattcctg tatctactaa tatcttagct gtatcttctg ggattcttgc
    16861 taagaaaact tcttccaaag attttccctt ccaatatgga aatatttctt ttcttataaa
    16921 ttctatttgt tcttttgata ttacatatgg gtcttgagtt cttttatcaa aagtatccat
    16981 ttctttgtct acccattgcc aagaaaactc tggtgtcaat atccctgttc ttctaaattt
    17041 tcctgctgta cctactatta attcattttc aaagattttt ataggaagtt ctttacatgt
    17101 ctccaaaaaa gctttagctc ttcttataca gatagcttct ccctcagttt ttttatgtga
    17161 ttctgtaaaa attctagccc tttcacaaca tatcataggt ttagaattaa tatattcttg
    17221 tctaaatgct tctattctat tagtttgact catttaaaat ccccctttgg ataatatata
    17281 ctacattttt gtatgttata acatacttta attatattat actgtccttt agcataaaat
    17341 ggaagcaaaa taacttattt tttttcatct accttgaaca cctatatatt aatacttttt
    17401 ttgttaaatt tataaaatct aaaaaaattt tctatgatat attttctata ttgttatagt
    17461 tatgtctaat atactaatga tttgaaatat caaatttgta tactagactt agttttaaaa
    17521 ccttagttga ttgtttaact ttgttaactt caacatgtat atccttcata taaggaagct
    17581 ctacaacacc tactccctta ttaaagtctt cttcaaccat agaaaaaatt tctttctcag
    17641 ttaagttcgt atcagtcaat gatttaaata aattgtatat aaatttaaca tatttatcac
    17701 tctcagataa cttatcatct tctggatact ctttagcaca gagaatatct attgttactt
    17761 ttccttcttt atcgtcttca gacatagaat agaataaact tcgaatattt ttttcaggtg
    17821 gtttttcagc accatttata gatactacaa agctaccatc tgattttgag ttagtaatac
    17881 tataaccact gtctattata gctttcgaaa atctttgaga taaatctttt tttacttctt
    17941 ctctaccttt cgcatcatta atcttttgtg agtaattatt aaatagctca atagattcat
    18001 cttttttatc ttcaacttgt tctttcaatg aatctttact aatttgttct gagctactta
    18061 agttcttatc agatacagga gacatacacc ctaccatcaa tgctaagcta agatttaaag
    18121 ccacaattat tgccactcta tttatattcc tttttacttt catacacaat cctcccatca
    18181 cactaaccgc tctaaagtat tattcattgc actagaatat atttatattt tctttatatt
    18241 aaagatttat tattcaatac atatcacctt cctatccaat tcttataact tactattagt
    18301 aaaattataa gctccttaat acagaaataa ggatgttatc ttttgttatt aaaagataac
    18361 atccttattt ttttgtatca atacatatgt cccaactggt aacttaatcg ccccaactgt
    18421 cagtatcttg gtccaatggt atatatatat ttagtataaa tttatcgata ctattttcaa
    18481 ataataattc tccattatat ttttgtaaag aagatttaat actctgaata cctattccat
    18541 gtacgaactt atccattttg tttgttaaca atatattttt attaaaagta agctgattta
    18601 gtttactatt ttcacattta agtacaaaaa atgaccttgt tatagttcct ttaattctaa
    18661 tatatttatt aatattttca tccactattt tatcacatgc ttctattgca ttatccaaaa
    18721 tattagaaaa tatgcttgac acatctatag gttttacaaa atttactttt gaaaaattta
    18781 tagagcaagt aaatttaatt ccttttttta aacatacttc actcttttcg ttgattataa
    18841 tgtctaatat catatttcct gtattataga tatatttaaa tgtttttatt tcatctttta
    18901 aattgttaac atattcatta atttctttgc tattgtttct aaggttgtct atacaataaa
    18961 tgtgattatt tatatcgtga tatagtttct taactcgcat atgagattct tgaatactta
    19021 aataatgcgc atattgcata tctaacttat tttttattaa ttcagcttga gattttaatc
    19081 tattctcttt tattatttct ttaaacaata gtataagaaa tatactacta gctcctacca
    19141 attttggatt aacaaaatta tcaaaatagt atccttctga atgtaatgac ccaaaccgtt
    19201 ctaatttttt taatacataa tttacaagca tattcattgt attaactaat aaggcaaata
    19261 ataaatatac ataatttctc ttatcttggc taattaattt tatagacttt ctaaaaaagc
    19321 taaatataag atatataaga ataattatta aattactata tatctgaatt tttatagatt
    19381 ctaagtaagg tactaataaa gattctttag atgaaataac attacctgta attgcaaaaa
    19441 atagtattct atatataaca gcatccaaac atttataata tacactaaat agataaaaga
    19501 aaatggaata cttaatataa cccatcttat aaaaataatt atagtatcct ccccaagcaa
    19561 taaatgctaa tatataaaat aaatagttta tattactaaa gtaatttcct ccattaaata
    19621 atctacttat ctgaattata atataaataa gaaacaagac aatatagaat actatttttt
    19681 catttttttt actatccttt ttataaatta aaattccgtt cattatagaa aactgtataa
    19741 atatagataa aactactaat agctcagtca taaagtttag catattgtat caccaagaaa
    19801 ttttaagaat ttctccttaa cttctttata tctatatcta ctaataggta cctcttctcc
    19861 actttccagt attgcagtat taggctttat attttcaaca tatcttaaat ttactataaa
    19921 acttttatgg catcttataa atttatatgg atttaattct ttttctattt tgcctaaact
    19981 atattttata tcaaaattct tgtttattgt gtgtattagc atatcttttt tttgaacttc
    20041 tatatatttt atttcattta aataaatctt atatgtatta gacttatttt ttattgtaat
    20101 atgactttcc ttatttattt caatatcttt gatacaagtt agcacatgtt tttttagttc
    20161 ttccaactct attggcttta gtaaatacct ataagctctt acctcataac cttcttgcac
    20221 ataatctatt aatgaagtta caaatattat ttccatttca ttgtctactt ctctaatctt
    20281 tcttgcagta tccattccat ttaatttttc catttttata tcaagaagta atatatctat
    20341 atcttttaaa ttactttcca gtaactcttc tccagatata tacttttgta tctcatattc
    20401 tatacttata tcttttaata tattctcaag gtaatcttct attaaaagtt gtgtttcttt
    20461 ttcatcttca caaactgcta tcttaatcat aaaatgcctc cttacataat ttctagttaa
    20521 tacaattaaa atatttataa tatatttaaa gataattttg tatatctgat actttaggat
    20581 aacattttta aattattttt atcaatactc aatcccatac aactattatt tgatatttca
    20641 atttaaagac agaaattact ataattaaaa acagtactta ctgacaaaag aagatgtctt
    20701 aaaatattaa aaaaataatt ttaggacacc ttcttttatt tatttaaact ttttaagcct
    20761 taaataatac attaatctcc tatataattg tactgcatat aaaatcaaca atataatctg
    20821 tagtacaata gcagttacag atgctccata accactatat aaatttctta tttcaatatt
    20881 caataaatat ataaatatta ctactaacat aaagaatata aacatattca taaataaaat
    20941 tgactttact ttatttgttt tatcagaatc actatataat ttaggaggta tgtctttgta
    21001 ttctttcgac catatatacc atgcagatgt acccataaaa ctaccaatac tcctaaatac
    21061 aagattccag ccatttgatt tatgttcatc aaaataatta ttctctacat catagtagta
    21121 atcaacagtg tattttattt ttcttggact tcccttatga aaataaaata ttcctccaaa
    21181 tttatttatc ttatataaat tatatccttt agcttcaata tcttctaacc atttctctat
    21241 cttatcaggt gaactatacc agtcaaattt gaattttatt ttcttttcca tagcactcat
    21301 tctccaaaat gatattttta aatagcttaa aaatctattt aaataattat atcattttga
    21361 tactaatttt aggttacaat catatattta aaagttattt aaatagtaat aaactaatag
    21421 ccataacacc cattcctgca atcaatccat aaatagataa atgatgttcc ccgtactctc
    21481 tagctgatgg caataattca tctagtgata tgaataccat aattcctcct acaattgcaa
    21541 atacaattcc aagagttata tcatttaaaa aatttctaag aagtacatat ccaataatcg
    21601 ctcctaaagg ctcagacata ccagataaaa aggaataata aaaagctttc tttttatctc
    21661 ctgtggcata ataaactggt acagatacag atataccttc tggtatattg tgtattgcaa
    21721 ttgcaattgc tatcggaata gctattgtaa catcatcaag agctgatata aatgtagcta
    21781 atccttcagg aaaattgtga attgctatag ccattgctgt aaatatacct gttcttaaaa
    21841 gagacttatt ttttttgata ttcttttctt ctatattctc cattttcttt atttcatgtg
    21901 gattttcttc ttgtggaatt agcttatcaa taacagcaat gatagccata cctataaaaa
    21961 aagctattac agttgaccaa cttcctaatt tctctcccat agattttgtc aaagcatctt
    22021 gtgctttagg aaaaatttct atcatagaaa cataaatcat aacaccagca gaaaatccta
    22081 agcttataga aagaaactta gtatttgttt ttttagcaaa aaatgctata caacttccta
    22141 taccagtaga taaaccagcc aataatgtta tcaaaaatgc aaatattaca ttattcaaat
    22201 aaatcacctt ctttttaatt gatattgatt atcattttct ttatgctatc atatatattt
    22261 tatatagtca atatttataa aaaatataca caataaaaaa gcagagatat aaacttagtc
    22321 tctgcttttt ctttatagta tattaaaatt atttatttaa gaaatccact gcatattgca
    22381 catataaagc agttccaatt tctagagcat cttcatctat attaaagcat ccatgatgat
    22441 gaggataaca agcacctttt gactcatttc tagctcctac aaatgctagt gcaccaggtg
    22501 ctatattcat aaactcagct aaatcttctg ctcctgttac tttttcaaat agtgttatac
    22561 agtcttctcc aaatagctta attgctgcat ctgtagctat ctttgaacat tctttgtcat
    22621 ttataactgc tggtgttaag tacccatact ctaattcagc atctgctcta tatgcttctg
    22681 ctgtagattt tgctatacgt tctaaaattc caggaatttg ctttctaagc tctggattaa
    22741 atagccttat tgtaccttct aagatagctt cactcgcaat tacattaaat ctagttccag
    22801 aatttaaaac tccaacactc acaactaatg gttccagagg acttacttct ctactaacca
    22861 tagattgtag attcataact attgctgaac ttgcaagcac tgcatcaaca ccttgatgag
    22921 gaagtgaacc atgaccacct ctacctttta cagtaatttt aaagaaatca gcagaagcca
    22981 ttcttggtcc ttcttctacc gatatggtcc cagattctac atcagtccaa agatgtattc
    23041 caaacacact atctacacct tccatagcac catcttgaat catagctctt gcacctttac
    23101 caacttcttc tcctggctga aaaaataatt ttacagttcc attgatacta tccttaatat
    23161 catttaacac ttttgctgcc cctagaagca ttgatgtgtg accatcatga ccacaagcat
    23221 gcataagtcc ttcattttta gatttgtatt ctacatcagt gcattcaaca acttgtagag
    23281 catccatatc acccctaagt gctacagtct ttcctggatt atctcctttt atagttgcaa
    23341 ttacacctgt acctccagct gatacatatg gtatccctat tttatcaagt tcttctttaa
    23401 ctctctttga tgttctaact tcttccatac ttttctcagg attttcatga aattcccttc
    23461 taagcttaat tacatagtct ttataactct ttgttatttc tttaatatcc attctatacc
    23521 tccaagtagt acacattctt cttatttaaa atacttttta tcaattaaat actatattag
    23581 atttacaaag atacctgcta ttactacaga agctattgta actgtagtaa atcctccaac
    23641 aagcatcttt ggaagcattt tatccattaa aaattctttt tcttctggtg tttcagccaa
    23701 agcctttact gcctcttcag ttaatatata gtttggtgga aaaccataaa gtgatgtaag
    23761 cgaaactgaa aaagccattt ctttactttc acctaaaagc ttacctacta taatagagaa
    23821 aattcccata cctattacac caaatattat gattcctaca agaggaactg ccatttcaac
    23881 aagcattgct ggtgtagctt ttgctaaccc tgcaaatata aaagccatca atgatgtcat
    23941 taaccatcca aatgaaccag atttattaag tggttttcta tcaactattc caagttctga
    24001 tgctattaca ccaaatatta aacatataac atatcttgat acaacttcat ttgtaagatt
    24061 tgtaaattgt actgctaacc atgctacaaa tcctaatcta agtattatca tatatgtcgt
    24121 atcatatttt tctggtaagt ctggtattat cttaaatctg cttttttgtt caggtaaatc
    24181 cttaactttt tcttcatctg tatttacagt tacttttcca cttctaaagt ctgataaaag
    24241 tctttttcct tcttttttaa gcattaatgc tgtaatagga taacccgcaa atccttgtgc
    24301 aacatacatc aaaattgcaa gtactgataa acttgcaaga ccttttgctg tagcagcatc
    24361 tgccatcatt atagatgcaa ctagccctcc agtaagagga ggtgtagcta ttactgctgt
    24421 atcccatcca aatactactg tccctaatgc caaagcacct acacatattc ctactattcc
    24481 tgccaaggct attactattg ttttccattg acctgctaat tccttaatac tcatcatagt
    24541 tcccatatga gttattaaca aaagcatacc taattgagct aatggtgttc ctaaagcggc
    24601 taagtccaca atattttctg ggaaaaaagt ccaaaatcct actataaata taattgctga
    24661 tacaaaaacc gacggaacaa aagctttagt ttttgctgaa actatatctc ctatcgtcag
    24721 tgatactaaa actagacaaa aagctgttat tgcctccata caaaatcccc cctcataata
    24781 aatatatttt tataaaatgt atgtatagtt atccattatt ttactacttt tgaaaaacat
    24841 ttcaagtcaa aattcgattt ttttcataaa attcgataaa ttccataaat taaagttagt
    24901 tttctaaatt attctttttg taaaaacgtt atcccgacca ttggctattt aactctattc
    24961 ttacaaagag aattaaatgt aatttgcaaa aaataaaaca taggatacat tttattcata
    25021 aaacatctta taaatataaa atgaattgtc tttatgaatc ttaatatatc ctatgtttgt
    25081 aagtttaatg tgtatgttag ctatggtgta aaatttgtat actagttatt gtgtaaccta
    25141 ctatatactc catttttgtt tactagctct tcatgagtac ctctttcttc aattccttta
    25201 tccgtcaata ctatgatttc atcagcattt tttatagtag tcaatctatg tgcaacaact
    25261 aaatttgttc tatctttact taattcctct aaagactctt gaatttctct ttctgttaca
    25321 ttgtctaaag cagatgtagc ctcatccaat attattattg gaggattctt taaaaagatt
    25381 cttgaaattg aaattctttg tttttgccca cctgataatt ttacgcctct ttctcctata
    25441 taagtatcat atccatctgg caaagtctct acaaagtcat gtattctagc ttttttagca
    25501 gcagctatca tttcttcatc tgtagcatta gggttcccac aaagaatatt atctcttata
    25561 gttcctgtaa ataaaaatac atcttgttga actataccta tattttttct caatgatttt
    25621 aaactaacat cttttatact ctttccatca atttttatat cacccttatc aaattcataa
    25681 aatcttggca atagattaca taatgtagtc tttcctcctc cagaaggtcc aactaatgct
    25741 atagtcttac cagcttttat agataaactt aaattatcca aaacttgagt cttatcttca
    25801 tatgtgaaag atatattttc tatctcaatg tcacctttta cattttcaag ttcaatagga
    25861 tttttagctt ctttttgatg gtcttgttcc attatctcta tgaatctttt aaaacctgtc
    25921 ataccatttt gatattgctc tgtaaagttt attaattttt tcataggttg tataaacatt
    25981 tttacatata gtatataagc agcaaaatca cccaaagtaa tctttcctat ataagtaaaa
    26041 taaccaccag ctattacaac tacccactct aagatatcca tgtataaatt cataccagag
    26101 taatattctg ccattacctt ataagaatct tgtcttgctc ttctaaataa tttattgctt
    26161 cttacaaact tatttaactc ttctttctca gtacaaaaag acttagttac cttcatacct
    26221 gctatactat tttctaaagt agcatttaca tcacctatag taactctact cttcataaaa
    26281 gcaatattca tcttatcttt tttagctatt gcaaaccaaa ttataaacgg taatatagca
    26341 aatattataa gcgtaagagg aatatttata tctataagta taaagaatga accaaccaac
    26401 attactatag aaatgaataa atcttcaggc ccatgatgtg caagttctgt tatatccatt
    26461 aaatcattta ctattctaga catagttact ccagatttgt tattatcaaa atacgtattt
    26521 ggaagtcttt gtaagtgtgt aaatacctca tttctcatat cagcttgcat atctacacca
    26581 ataacatgtc cccaatactg cataaaatag ttaagccctg cttttgctat aaatataagt
    26641 ataagtgcta ctgcaaaaac tactaacatt cttaagtttt tatttggaac aacgtcatcc
    26701 attatatttc tagttatcat aggataaact aaatcacata aggaaaatag aaaagctgct
    26761 ataaggtcta gtgtaaaaat tttcttatat ggcttataat atttgacaaa ttgttttatc
    26821 ataagtatta aatccctcct ttattagtac ttctaaaaaa tacccataaa ttagtataac
    26881 ataacaataa ctttaaatct tttataaact tacaaagtaa taacaatctt tttcataaaa
    26941 ttgaagattt atatcatata gttgtatact tgaaacaaca taaaaagagc tatatctcta
    27001 tatttaaaat tttataaaga tacaactcta tatttaatac taaatttttt ttataaatac
    27061 aaaactactt caatgcattt aatatctttt tatctaatgc tgttgtattt atacctgtta
    27121 tatatcctgt tgcctttatt gtaggtatat cataagttgt tggtagtata gtagtagata
    27181 cgattaaatc agcaccttct tgttttgttg aacattcagc aattttacat tgctcaactt
    27241 ctgcttgtat attattttct ttaaccaatc tttctactct gtcacaaact attgttgatg
    27301 ttgcgattcc tgctccacag gctactaata tttttttcat atattgtcct cctacaattt
    27361 ctttaatatt ttataactca aaataaaatt gataaatttc ttatatcttt atttatataa
    27421 ataattatat cattttatat aagaagaaca tcttaaaatt attcttttaa tatgtttttt
    27481 atttcaacat tgcttatact taaaatttca ttttttatac aagactctat gtactttgat
    27541 atatttttat aatcatttaa attaaactgt tcaatattca atttatattc tatatcactg
    27601 acaataccta tcaaatcaga cttattagat aaaatatcct catacttacc ttttctagtt
    27661 acttccaatc ttttaaatct ataattttta tatccttcta tgataatcaa gtccatatct
    27721 tcatataatt gaatcatttt atataatgga ttcttctctt ttagttcttc aatatatgct
    27781 actcgtttat ctgatattat acagactttt ctcgccccag attttctatg tttataagta
    27841 tcttttcctt cctcatccat atcaaaatca tgacaggtat gtttaataac tccaacacga
    27901 tacccttttt tatttaattc ttttattatg ccttcaatca atgtagtttt acctactcct
    27961 gaatatgaga caactgacaa tatattatac ataaaattgt gtcctttcat aaaactatct
    28021 aataacaaca tccactaatt gattttcttt tattgtctca ttctttttaa tcttaatcat
    28081 acaattactt cttagagttg cagataatct agcttttgca tctttcttat catttagctc
    28141 tacatacaat ttaccttcac ttgtatatag ttttccactt acaaatctat ctttatttga
    28201 aagtttatta aattctcctt taaatatagc ttttatatga tgtgttccct tcttatatgt
    28261 taaatactca ataacaggaa ctatcacatt atcaaatacc actattgctg catgtggatt
    28321 accagaaaga ccaaataata gcttttctcc caatgtacct acataacaag ctccaccagg
    28381 tttaatgttt atcttccaaa ataattctgt tgcccctatt tttttgaata catctggaat
    28441 taaatcttta tctccaacag aaacaccacc tgtggtaatt aaaaaatcta cactctgtaa
    28501 catattcttt accttttttt ctaactccaa tatatcatca gaacaattgc atattgtatc
    28561 tgcttgtatt ccaagtttta ataatcttga atataaggta tatctattac tatcgtatat
    28621 tttaccattc tctaaagtct catttatatc ttgtagttct gtacctgtat tcaaaattcc
    28681 aacttttaaa tcatcataaa cagttacttt ggatattcca agacttgcta atactccaat
    28741 accatcatat cctatattct gatttttatt aataatcaat tgcccctttt ttatatcctc
    28801 acccttaaat atgtaattat cgtattcctt taattttata tttaaagtta tcatatcact
    28861 tataactgtt acatcttctt tttttattac acaatttgca ccctgtggta tctttccacc
    28921 agtcattatc ttcactgctt catttttatt taatacccta ttaaatacat ctcctgcata
    28981 caaagtccct ataaccttta aatttctatt ttcagaattt gcattatatg cataaccatc
    29041 atagggtgac ctatcaaatg gaggactatc aattgttgaa tatatatctt ctgcaactac
    29101 cttatttact gcatctataa gatttactat cttgaccctg tttattggta aaacattttt
    29161 ttcaataatt tttatggcat cttctaattc tatcatttta aatactcccc aatatatttt
    29221 ttatttaaga tatttctatg taatatatta tagattataa aagttggaaa tacttttatc
    29281 ttatatttaa ttataactaa tagttatcta taagtgcagt tcaaatttta taaatctaat
    29341 aaaaaaactg ttcccagcta gaaatttcct tctatcttga aacagcatca tatgctttag
    29401 aatattttat tctaataaag ttccttttat tatttttaca attttatcac tttcaatagt
    29461 ttttatagat tttattttgt tatccacaat acttattgta agtacctctt tttttaatat
    29521 tttgttatct tctttcaatc tataactctt aactacttta ctgccttcat gattatatgc
    29581 tgttaaatca taattcgcat ctcctacaat ttcatttatt ctggaatctt caacaccaac
    29641 atactctccc aggtcaatat gtctaaaatt ttctagtgat acagccgatt tttcatggtc
    29701 acttttttca atcactatac tagattcagc atcaatgact gatatacctt gtgatgagaa
    29761 agaattggtt tcaacattga caactttatt ttcgttaata tcaacatata aagcacttcc
    29821 atctagttca tcatttccag tatattttgg ataaactaat gtcatataac ttgatgtatt
    29881 tatatcttgc attgtatttg attcttttaa aactttaggt tttatataat aaaatgtatt
    29941 ataaggtata cccatattct ttataatgta cttataatct ttattcataa tttcattaac
    30001 atcattttga acttttgtca tagttttttg ctgtgttgct ttacttgcta cttgattttt
    30061 ctctttagta ttttctttat gacatccaac aagtataata cctaaaatag ttacacaaac
    30121 taatgacctt tttaacttca taaatcaatc ctcctttctt aaattttata gtatttatct
    30181 atatctcaat ttacagttat ctcttttttc aataattata tttattatat tataaaataa
    30241 ttatataata tttatttgaa tttaataaga ttaagaagct aattgtaatt tttctgtaat
    30301 aaaaaacact ttaaagttga attttgaaaa ttcaatttac gatattttta ttgtaagtac
    30361 cttacaacat tataccaaat tattcctaaa caatcaaaaa ctccatgaat atttaaaggt
    30421 tttctatttt aaaaatcacc tttgactatt aatttatcaa atatgttata atagttttag
    30481 ataatttaag gaggattaaa taatggattt taaacaactt gaagtatttg ttgctgttgc
    30541 aaaacatcag agtttttcaa aggctgcaag agaacttttt ttaactcaac ctactgtaag
    30601 cgcacacata cagaatttag agagagagct agaaactgtt cttataaata gaagcaacaa
    30661 agttattaca ctcacaaaat ctggtgaaat tctttatgaa catgctatat atatattgaa
    30721 taattgcaaa cgtgcaattt atgatataaa agaatattca ggaaaaatag aaggtattat
    30781 agatatagca tgtagttcca tacctgaaac ttatatttta cctgatttta tgaagagttt
    30841 ttctatgagt tatccagatg taaaattttc cataagccat tatgattctc aatatgctat
    30901 atcagaaatt ttaaatgaac gaataagctt tggattagtt ggttctaaaa tcaataatcc
    30961 tcaaatagag tacctagact tattagatga tgaacttgtt cttatcactc cttcagattt
    31021 taaaattgat aataaaaata attgtatcga tataggagaa ttggcttatc taaattttat
    31081 tatgagaaaa gaagggtcag gtacaagaaa cttaatacta aacacactta gtaaaaataa
    31141 ctttccagta agtaaattaa atgtaatcgc tcatgtagaa tcaaatgagg ctattaagga
    31201 aatggttaga ttaggtcttg gagtgtcctt tatatcgtac atttcagcta tcgattatct
    31261 caatgcaggt aaaataaaat gttataaaat aaaggatgtt gattttacta gaaagttttt
    31321 cttcatttac tctaaaaaga aaactttttc cccattagaa gataaatttc taaacagatt
    31381 atgtgagtat ttcgaaatta taatataata actttaataa agtagtgctt aattttgagt
    31441 ttataactaa ttaataagct aattataact atcacataaa tgcttataaa ttattatgaa
    31501 ccatctttct tttttattta atttagtcaa aaaatccctc gttcaaatga acttgggatt
    31561 tttttattta tactactcca atagcagcca atactataca aataatcata actgaaatta
    31621 atatcgtagt tgtctttacc ttttttccta ataaccaata cataataccg aatatcatag
    31681 ctggaagcat acatggcatt atttggtcta agtaagtttg aataggctct gcccattctc
    31741 cactacctac attaacacta agttttaaca ttacattaga tgcagtcata cctccaatta
    31801 ccataagccc taacattgaa gctgcttcca taacttttga aactatactt gaaccactca
    31861 catctttaaa gaattgagtt ccatacttaa atcctttatc tagacatata tagcgtataa
    31921 taaaatgagg aacatttatt attaaaagga ataaaatcgg tcccattatg ttaccctgag
    31981 aagctaaagc tataccaacc ccagctgcta taagctttaa tgttccccag aaaaatgaat
    32041 ctcctatccc agaaactgga cccattaaac ttgatttaac agcatttata ctattagcat
    32101 caaattcttt attctttgca ttttcttctt ccatagctcc agatatacct accaaaagag
    32161 tacttatatg aggagtaact gacataaatt ctaaatgtct ttttaaagcc ttagatttct
    32221 tttctttatc atctttatat aatctacgga ttattggtgc catcatgtat gcatatgcta
    32281 tattttgcat acgttcgtag ttccaagatg aatctaatgt caatgacctt ctaaaacatt
    32341 tccaaaagtc tttcctcgtt attttactat ctggtgaaat tgtctctaat ttcttagaac
    32401 tcgttgtcat ctactattac ctccttctct cctacaaagt taacatatat taaagctatt
    32461 gctaatccaa acaatgcaac tcctgttact gttatgttta ggtaagcaac caatataaat
    32521 cctaataata aaaatgctac tgttttctta ttaagcatca ttctagctaa catggcaaaa
    32581 cctattgctg gaatcacgcc tgctgcaaca tccattccat gagttacata tgatggtata
    32641 aatgaaagta ttttttctat tactggaaca cctaatgaaa atcctatacc tactattatt
    32701 gctaatatta atttatttcc tataccaaat aataattgca cacgttctac gccttcaata
    32761 ttcccctctt ctgcataacg gtcagcaata tgagctcccc atgtaagtac tactgtcatt
    32821 aagaagttat ctataagcaa tactagagtt gctataggaa ctgcaagtac aactgctgct
    32881 gatacatctt gacccgttaa tattacaaac gctgtggcaa taatagaacc agacacaaaa
    32941 tcaggaggat tacttgcccc aacaggaaat gcacccataa atactaattc taatgttgaa
    33001 cctattacta tacctgtatg aagattacct agtgctaaac caacaagtgg tgcaacaact
    33061 ataggtctat tatgcattgt agaacctaca aaactatgca tatatgcaaa gaatgcaatt
    33121 agagttatca aaataacttg tgtaaattcc attttcatcc ccctaaaatt atattaattt
    33181 cattacattt gtagcatcat catcaggaac taattgtaca aatacattta cgccttctga
    33241 atctaactct ttaatcatct ttatatcatc ttttgataca tgcactgctt tagatatatt
    33301 ttcacgatta tcatctgatt ttgttccacc aaggtttata gatttaatag cttttacctc
    33361 tttagcaagt ctgtaaacat cttctacaga ttcacaaagt atcaataagt tgtatttatc
    33421 agtaacacct gagttaagtg cttttataga atctgctatg cttttcatta ctaacttaac
    33481 attagatggt ttagccattt taagtcctgc cattcttaat tcatctttta ataagtcatc
    33541 acttgctatc aatatacagt ctgtacttaa aaactttgtc catgtaaatg ctacttgtcc
    33601 atgtataagt ctgtggtcta ctcttactaa tttaatcatt ataattcctc cctaattttt
    33661 ttgatttcat ccatgaatgt ttttacgtgt tctacactaa catccccaaa atctttgtaa
    33721 ttatctttaa agtaacttcc tattattgcc atatctccta atttaagctg tttttttgca
    33781 ttttctaatg taactccagc agctataacc aatgggaagt ctcctaaatt tttacgaaac
    33841 agttctattt tttccattga tgtttcttga cctgtagcat tttctgtaac tgctatagca
    33901 tcacatcttg tcattcctat ttttaaatct tcttcaacat cattttctga taatactggc
    33961 tgatatttaa aacgtacgcc tccaattaaa tacgctggac attttgaacg ttgtaattta
    34021 aaaaactctt ctaaagtagc ttcatctcta ggtttaacat gacctactac agaatcaacc
    34081 tgtatataac tagcattata ttttgttgcc agttcaaatc ccatagtatc aacatttaaa
    34141 cagtttactc catatggtat tgataaatta gcttttgata catattcaag tattctttct
    34201 aaatcataat aattaccata gtaattttct aacattattc catctacacc attttcttca
    34261 aagattgtaa tctctttttt agctctttca aagatatctt ctggagtatc tccttttaag
    34321 tgtatcattg ctataattgg ttttttactt ttaaatactg ataatattcc cttttttttc
    34381 ataaactaaa atacctcttc ttcttcttta atttgtaatg tcacgctttg tattcctctt
    34441 ctagattctt ccactaattg gtctatatct tcaactgtta attcatcaga aaaccttgta
    34501 agaagttcta ataccatatt caaattcatt ccagaaaata acctaacatt atgtagatta
    34561 gttaatctta acattgctgt acatacactt gctccatata agtcagataa aattatatat
    34621 tcacattctt tatcttctga tattgttttt tctaattcta aagcaaaatc tgaagcactt
    34681 tgtccagggt acaaaccata cgaactgaca caatctgcta aatctcctac tatcattttt
    34741 actgcattta aaagcccttt tgaaaagtct ccatgagatg ctaatattat ctttttcata
    34801 ctcatcctcc tactaaatta tagattatat ttctgctgat actgcatatt tgtacttatc
    34861 acttcttata aattgctcat tgtaatataa gacatcatta ttttctgtat acaccgattg
    34921 tcttactttc aatagtggca tattacttgc acatttaaga tggtcagaat attctttgtt
    34981 tgaatgtttt acttcaaata tattttctac tcgtttaggt aatgtgctta tattattaga
    35041 tataacctcc attaaagatt tatcttctaa ttcttctttt aataaaaaca tgtaatctat
    35101 tctaaaatag tcaacttcaa aaataacagg tacttcatct attaatctaa gtcttttaat
    35161 acatattacc ttctcttcta gttcaatact taaagcttca gctacttgct tatctgcttt
    35221 ttttattaaa acagagatta ttttggtact tggttttgca ttaatacgat gacaagaatt
    35281 agtaaaacta tttcctgcac atgttgcttc tattctctct ggtattgtta cataagtccc
    35341 tttaccacgt tttttacata atatatcttc ttttacaagt tcattaagtg catttctcac
    35401 agttatacga cttatattaa attctttaca taagtctcct tcagatggaa gtttatctcc
    35461 tggtttatat atgccagatg ctatttgttt ttttatgttg tcatatagtt gttgatataa
    35521 aggaacaaag gaatttactt ctaaatccat ttcttttttt tgtctctttt catccatttt
    35581 gctcacctcc ctatgtaaca tttttataac attatataac aagttcttta ttacatatta
    35641 taacaagatg aaatcgttgt caattttttt ttaaataatt tttaatttga cagtattttc
    35701 ttttattttc tctagtgtga caaattgcta tttttacata aaaataagac tgttgtacaa
    35761 cagattaaat tgtttataca acagccttta ttcacttatt acatttatat ttttacttat
    35821 aaatttatat taaaaaatgc cttatatccc ttatacattt catatatgtc tactttagag
    35881 acaatctcat gtggtgcatg catatttaaa actccaacac cacagtctat aacctctgca
    35941 ccttgatttg ccaatatatg ggctatagtt ccgcctcctc cttggtctac ttttccaagt
    36001 tcagcagttt gccatattac atttccttta ttaaaaattc tctttacttc tgacataaat
    36061 tcagcattag catcattaca tccactcttt cctcttgaac ctgtatactt agttaaaact
    36121 actccatttc ccatatatgc cgaatttctc ttttcatatg cttctccaaa atttgggtca
    36181 tatccagcag aaacatcagc tgataatacc ttactatttg ctaaagctct tcttacttta
    36241 atatcacaat aatctccttc taaagcaatc agttcagcca cagtattttc aaaaaacttt
    36301 gagtgcatac ctgtattccc tttagaacca atttcttctt tatctacaca taatgcaact
    36361 gaagtaaatt ctggttgttc agtttctagt atagctttta cagcagcata tgaacatacc
    36421 ctatcatcat gcccatatcc caacaccata gacctatcaa gccctaaatc tctagcctta
    36481 ccagctggaa ctacttcaat ttcagcactt aataaatctt cttcaactat attgtatttc
    36541 tcatttaaaa tctttaatat atttgctgtt ataggttctt tttcttcatc ttctaaaggc
    36601 atatttccaa ttaatatatt taaagcttct ccactaattc cttctgatag tttcttttgc
    36661 atttggtctc cagccaaatg aatcaataaa tcagttacac aaaatactgg gtcactatct
    36721 tcttccccaa tagatatgtc cacttttgtt ccatcattta atataactat accatgtaaa
    36781 gcaagtggaa tagccgtcca ttggtatttt tttattccac cataataatg agtcttaaaa
    36841 aaacctagat tactctcttg atacagtgga tttggtttta aatctaatct tggcgaatct
    36901 atatggcttc ctataatctt catacctttt tctatataat ttttacctat tacaaaaagt
    36961 gctactgcct tctctttatt tataacataa atcttatcac cacaacttat gcttcctttt
    37021 tccatagctt catctattga aatatagtta ttttctttag ccaattttat aatttctttt
    37081 actgaaagtc tttcagtctt tgacttactt aaaaactcca tataattgct agaatattgc
    37141 ataatttgtt caatagtatt ttcttttctt tcaaaatccc aagcattctt aaattcgtac
    37201 tttaagttca tattttcctc ctactaagtt ctaattttta ttgtttttca tgtattgctc
    37261 atattcttct ggtgaaacta ctttgacact atctagtctt gatctaaagt gggctctaaa
    37321 gttttctaaa tattctttcc ttaaaatttc tctttctttg atttcatcag cagaaaggat
    37381 tccctcttta tttttctttg ctaattcatt aatcctatct aatttcttct tgtcaaacat
    37441 tttttcctcc taaaaaacct ctatttattt aataaaataa tttattgctt tttatatcta
    37501 acttgttttt atattcacaa aaaaactcat ctatattatc acttattata gatattccct
    37561 tttctagttc ttccatgctt gggcgagcta tatttatcct aaagaatcta tcatccacta
    37621 gattgtcaaa aaaataagtc cctggaagca ttgacactcc tttgtttaac ataaaatttg
    37681 taaatgcttg agatgtatat cctcttggta attctaaaaa aaagtttatt cctccacaag
    37741 attttctaac ttttaattta ccactcaatt tgtcattaat caagtttttt gccaatttat
    37801 acttatcagt atatatcttt tctatatgac tcaaataatt cttccaatca aaattattca
    37861 tatagtaata cattgatttt tgaattaaac ctggtgttga tatatctgat gaatatttag
    37921 cccataaaac tttttttaac aactcatttg gcatttctac aattcctatc ctaagacctg
    37981 gcataagtat ttttgaaaag ctttttatat atactactct atttttatca tcataacttc
    38041 taattgttct attgtccttt gattcaaaaa caaaatcact tataaaatca tcttctatta
    38101 tataaaaatc atgttcttct gccaattcta tcaatttctt ttttttatat gttgagtaag
    38161 atattccagt tggattttga aaattaggca taatgtatat aagtcttggt tttatttttt
    38221 ccaactttaa ttttaaaata cctatatcaa ttccatcatc taacataggt atagatatta
    38281 tttttgcacc tctactttta aacacttcaa ttgctccatt gtaagaaggt tcttccataa
    38341 aaacaacatc tgaataactt ataagtcctt tacaaactat atctatacct tgttgagcac
    38401 ctgatattat ttgaatattt tccttagtag tttttatatt tccatcactt agatactcta
    38461 ccattttttc tcttaattct tcagagccaa gaccttcatc atattcaaat attgaactac
    38521 catctttaga taatgccata tttacagcat ttttaaatga atcgatagga aacatatcat
    38581 tagatggatt cccattgtca aaatgaatta aatcttctct ttgcacaata atcttatttt
    38641 tcctgttatc tataccgctt ttataagaat ttaaacttga tacaaaagta ccactcccta
    38701 taattttata tacatatcct tcattttcta aaagctcgta taccttaact atagtagtgt
    38761 tatttacttg taaaaaattt gatagttttc ttattggagg caatttttca tgttctacta
    38821 actctttatt tattatcaat tcttttatat gattaaatat ctgtatatat ttagttgatt
    38881 ttttctcatt taaatctatt ctgtattttt ccaaatttat caccgctcat tgtatttata
    38941 ttttattgat gcttttatgt atatttgata ttatttagta tgtatatata ataccaaaat
    39001 ttttataaaa aggggaaata tttatgtttt acgactatca catgcactca agtttctcta
    39061 cagacggaaa atctacaatg gaagaaatgg taaaaaaatc tattgaactc ggtttggagg
    39121 aaatttgttt cacagaccat gttgactatg atgtatatgc tgatgattct ttttcaattg
    39181 tctacgaaga ttattttaag agtttagaga ctcttcaaag caaatataaa gataaaatat
    39241 caataaaaaa aggaattgaa tttggagttc aaactcaatt aatggataca tacaaaaagg
    39301 aagcacatca atatccattg gactttataa tatgttctat ccatgctata gatacaatgg
    39361 acttatacct tggtaattat tttaaagata aaactcaaca tgaagtttat gaaaattact
    39421 atctttattt atataatatt gttaagaatt ataaagatta ctctgtctta ggtcatttag
    39481 atttgattaa aaggtatgca ccttatgaca ctattttaga tgatagacta ttttctgata
    39541 taattgaaga aactcttaaa caagctattt atgatggcaa aggtatcgag ataaatacct
    39601 cttgttatag atataatctt ccagacctaa ccccatctaa gtatatactt caaatgtata
    39661 aagacttggg aggggaaatc ataaccacag gttcagattc acatcatata agtcaagttg
    39721 catgtgagtt tgactacata tattcattac taaaaaatat gggatttaaa tatgtatcta
    39781 aatttaataa attgaaacca gaatttataa aattataaat ttttacacct ccttgtgaat
    39841 atatttaaaa gataaggagg tgattttttt gaaagtacct tatttagtac tatcaacagt
    39901 tattttattt atatccaatt ttattgtaag aatatttggt tttttatata aaatattctt
    39961 atcaagagca ctaggtgaaa caggtttagg tatctatcat atgattttta actttttgat
    40021 gatttgctta gctgttacaa caacaggtat acctactgca cttagttgtc tagttgcaaa
    40081 gagaaaagct ttaaacgata gacacaatac taatgcttta tttatatcaa ctttatatat
    40141 atcatttttc gtagcactaa ttatatctat agttgcatct ttcaatagtt cttttttatc
    40201 acttaaattt ttaaaagatg ctaaattaaa tctatttata ctagctgttt gtcctgctat
    40261 agttataata actctttcaa acgtactaag aggatattac tatggcataa agaatgtaaa
    40321 aatccctgct attggacaaa tcattgaaca aattggtaaa atattatttg tttttttact
    40381 agttatgtac ataaataata aatctatgaa ttgttatata gctctattag gtatatcaat
    40441 cggtgagttg agtaatatta tatttatgct tatttgctta tggcgagatt catcatttga
    40501 caataggtat attataagca taaaagattt ttataattct tctatggaaa cattaaaaat
    40561 gtctattccc ataacttgta atagaatgtc aaacatactt ttgcaatcta taagttctat
    40621 gatgatacca tcaagattag cattatcagg aatgacttat caacaatctt taagtatgta
    40681 tggtattgtc agtgggatgg taatgccttt tatattttta ccttttactg ttggttcagc
    40741 gcttattgta aatttaatac caactatttc acaagaaatg gctctaagaa agcgtaagag
    40801 tgtaattaaa aaaatcaaat actccatact tcttacttta tttgtaggca tactatcatc
    40861 aatatttttt tatttctttg gaaaagattt atgtatatta gtatttaaaa atacacttgc
    40921 aggagaatat ctaaaagcta tgtttttagt tccactattt atgtccctaa atcaaactct
    40981 atctggcatt ttacattcaa taagaaaaga attagcttca agtataaata ctattactgg
    41041 catgttaatt caactaatag ctttatatgt atttcttcca attccaggac ttaatattta
    41101 tgcatacata tacacaatga cgatagtatc aatctttact tgtctattac atactatagt
    41161 gctttttaaa tcactaaaat ctatacgcta gttataaaaa cgtgaaggct accaatgtaa
    41221 attatacact ttggtagcct tccttagatg atttttattt ataatttaaa ctgtctatat
    41281 atcttaccta tctatctgtg gcaacttggc tttccacttc catgattagg tccacattgc
    41341 gaaaaatcac atactttaga gaaagtatca acaagtcttg gttggattgg atttggtgat
    41401 gctaaaacta ctatttgttc acttattaat gtgctaactg tttcttgtaa gcaaagtact
    41461 aaatctatat catttggtat aattaattca tagaaaggtt cacaacattc gttgcattct
    41521 aattttaata tagattttcc tatacaatct acagttaagt tagtttgata ttcttgtctt
    41581 aaagtaacag ctcttccgtc agctggcaag catattggtg ctgaaagtgt gttaaaagta
    41641 aattctacat aatcaactct tctcttacat ccacctcctg ctctaactgc tgggaaagct
    41701 aatgcaacga actctgtaca tccacatcta cctcttagct ctagtactaa attacgaact
    41761 gctacagtta atcctctttg tttataagct actgatttgc cttttccttg tctacagcac
    41821 tcagcgtttc tttctccaca aactgcaaat tcaaaagttg tttcacagtt tcttcctact
    41881 atatctccta atgggtctaa atcgaaatct tctaaagttg tcattcctgt gtctataacg
    41941 attccatcat tacttaagca tattttttct atagttacac ttgcttgacc tgcacttgga
    42001 actgaaccaa atacttctac tacttctgtt tcaaaagtta atggctctcc atttggtcct
    42061 gttgcatctg taaatgtttg gaattgcata gtatcaaata ttctattagc ctctattgca
    42121 aatggcactg catttgatac agagaagttt cttggagata taggattaca gcattctggt
    42181 ccacatttgt tttcaaaaca ttcatctgaa tccatttcac atggttcaca atcacattta
    42241 caattgtcat ggcatccaca attgtcatca catggattag gtttgcatgg attgcatggg
    42301 ttaggtttac atggattaca tgggttgcat ggattgcaat catcatggca ctcacactta
    42361 ttatcagtat tacatccatt atgatggtgt gattttttaa aacctctcat gtcttctgaa
    42421 tttatttcat ctgtatacac ttcttcttca ttcattgtag acattggctg attcatcatt
    42481 cttcttttat tttttttata atcttgcatt tttcattgcc ctccctgtaa gcgattctta
    42541 ttaagtcttt ggctttttga agcctaatta atactatgct tttataatct taattgttac
    42601 ttgctttttg aatttatcaa taattaggcg tatttagtag aaaaatgcat attaataaaa
    42661 aaagtactac tatttgaaat agtagcactt tttttgtccc gaaacaccgg tggctaagta
    42721 aattcacacc ctaggcactc ccaggtgggt gacttgccag taacctttaa cgtccaaact
    42781 aactaggcat gttataattt accagacacg gtctcccgta tttcatttgt aggttgaatt
    42841 taaaaaagcc ttatcgtatg tttcaggatt tcatattatt attatataat aaattttgaa
    42901 ttttttaaag tggttttata tagtaattga gataatctta ttctatcatt actttttctt
    42961 ccttttttct ttatgtattt agttaattat cgttttttct tagctctagt tttagtattt
    43021 ttttcattat aattgtattc ggattcttgc tcattaatgt tgtatagttc ctcatcatat
    43081 actatatctt catcattttc tatattttca tagcctgtgt tcttatcttc tactaattca
    43141 tcttcattat cctccatttc accttcgtct gttaactcat cataatcatc atccaattct
    43201 ttgtcttcac aatctaattc ttcgtcttca taatttaact catcattatt gtcttcatca
    43261 tcattattac tatcatcacc tcttaaaaga cttcctaaca atttgagatt attcatacta
    43321 ccaagattgt ttatatttgc caaagagctc aaactaaatc caccttcacc aaagtcatct
    43381 ggtaaaaata attctgcaaa taaatctatc ttctttttca ttcctatagc tttttcaact
    43441 gaattttttc ttttttctgg gaatacatct ataatttcct ttatcatcat attcttttta
    43501 tcatcactat ccatttttct gaaaaaatcc gtttcatctt ctgtatctac catatctata
    43561 ttatttaatt tttttattcc tctaactaaa tctaaaacac tttctatttt tattagtatt
    43621 tttctttcat ctcttgacat gtatttttta gttcttgtta ttatttccat tcccatttct
    43681 aaatcttctt cacttaaatc catgagcctt aaacctcttt ttatatcatt tgcatcaaac
    43741 cttaaattgc catgtgtaga ctgtgaaagt ttatcatttg catttttttt gttttttcta
    43801 actctattat ttccttttga tttattttta tcatcaaata tagagatatt accattactc
    43861 aatgcaatca ctccaatcaa aaatagtaag ttattcaaga caattccccc ttatattata
    43921 taattgccct atattaaagt atgtttttca tattttaatg ttccattttt aaagctcagt
    43981 gcatatatat tatctataag aatttttatt tatattatat atcactcaca attcaccact
    44041 tcaatattat ttctatatat aaatagtatg aatataattc aatatatatt tatagaaagg
    44101 ggttttttta tgaaaaaatt agatacagag tcattagaaa aaatggctaa acaaaaaaat
    44161 attgacaagg ataaaataga aaagatggct gatagttaca aaggaaaatc agaaaatgaa
    44221 cttatggagg aacttataaa aataggtaag aacctagatg gcagagatga agtagtatca
    44281 aaattcaaag catttttaga tgaaaatcaa cgcaaaaaac tggataatat aatggaaaaa
    44341 atctcagatg cagaaaacca aagagatact aaaccagcta aaactaagaa ggctaaacct
    44401 actaaaggta agtcaaattc acctgcacca aaaaatacac caggtagtca acaaaaaaaa
    44461 tctaaaagtt tatttaagaa aactaaaaag tctaaccctc atgaatagat aaaaataaac
    44521 tatttaagta attagataat tatttttagt taatctatta ttaaatttga tattattgac
    44581 ataaaaaatc aggtagtgcc ttaggcacta cctgattgat tttaatagtt ttattttttt
    44641 ctttctgtaa cactttctct atcaacaatt ctataaggta attctatttt cttattctca
    44701 attaattcct tatttattaa ctttataacc attcttattg ctactgctcc catatcgtat
    44761 agaggttgat atactgtagt aagtttaggt ctatacattc tagctatctt aacatcatta
    44821 aatcctgcaa cagatatgtc ttctggaacc ttatatccag catcacatat agcatttata
    44881 gcacctatgg cagcctcatc acctgttaca aaagccgcat gaggtattat tccatcatct
    44941 aatagttctt tcatactatt gtatccactc tcataatcag ttccaccata cttaatcaaa
    45001 ctcttgtcta attctatatt gttatttgaa agtgctttct cataaccagc aagtctttcc
    45061 atttctaaaa cagtatcatc tttactcgtc attataaaag ctatcttttc atgacctttc
    45121 tttataagat gctctgtcat atcaaaagta gcttcttcgt tgctagtact tactgtatat
    45181 atatcataat ttctagcagt tttacttata tatgtcgctg gtattccaca attttgtata
    45241 tagttgatat gttcttgttc aactatccat gaaatcataa ctataccttc cacttgtttt
    45301 gctctcaata gatttatact cttaagttct tgttccttat cagagtatgt attcgctaaa
    45361 agaatatcat agtcatacat tttagcaacc tcttctatcc catttaacac ctcattaaca
    45421 aaagaatctg aaacttctgg aactattacc cctattaatt gactcttctt tgttactaag
    45481 cttctagcaa gtggatttgg tatatatcca gtctctttta taacctctaa aactttttgt
    45541 ttgacttcat cagttacagg ttttgaatca tttataactc tagatacagt agatattgac
    45601 actcctgctt gtttagcaac atcttttatc gttatattgc ctttcatctt catcctcctt
    45661 attttactaa atttatctaa aactgaacca aatttttgaa ggttcctatt ttaactacat
    45721 ttatataagt ttttatttta ccataaaagc aagtacattt aaatacatta tattttatat
    45781 tataccctat attcaacaaa tttaataata ttcattctta atttaaatca tattaaaaaa
    45841 taaaataaaa aaatgctggt ataaaatacc agcatttttt tattctaatg atttaagtat
    45901 tagcagccgc aacctacttc accatcaaag aatccaccac atgaacaagt tattagcaat
    45961 aatattaaga aaggtatcca tgacattatg tcaatgtctc tccagcaatc ttcaaaagct
    46021 aagaatagga agaatagtac tattatccac catcctcctc caccaaagca tccttcttct
    46081 ccaaaaaatc tatctaacat aacaaagccc tccttttttt aataactgtg ttcttattct
    46141 aagcatccac cacagcagca aagtattaat aaaactatta ggaatggtat ccatgccatt
    46201 atgtcaaatt ctgcccagct atctccaaaa gctaagaata ggaagaatag tactattatc
    46261 caccatcctc ctccaccaaa acatccttct tctccaaaga atctatctaa cattatacgt
    46321 tacctcctgt tgatattttg ttgagctttt cgctcctaac atatactatg catttggttt
    46381 ccattttgct aatgtatttt tactattttt ttaaattttt taatttgaac taaaaaaggt
    46441 aaaaccataa cttgtataag ttactggttt tacctattta ataatcaata ttttttagca
    46501 acaacaacca tcatcgaagt taaataataa caatactatt aatataaccc atatccatat
    46561 attgtcatcg cataataagt cttctaaaat gtctgtgcat aatagtaaga atactataac
    46621 tattattatc aacattttat tcccaaaaaa tccttcgcat cctccaaaga aacgttccat
    46681 attgtcttct aattttgtca taactaatcc tccttgaatc attaagagac taagttttgt
    46741 ctctttaatt aaatatatgt agtaatagac agataagtta cttctaaaaa gtttttattg
    46801 attagtttat ttatttcttt ttcaccaagt ttattgtatc tatcaacttc atccagataa
    46861 tcttttttct cctctgatat agtatagatt aaactttcta ttgattttat tttgtactcc
    46921 ttatatcttt ctatcaagaa ggatgttttt gaattttcta ttcctttctt tacatgcatt
    46981 aaccttgata tgtagtcttc tttttcatta gatacataac tctccttttc agctacatct
    47041 tcaaggatta aattggtctc cattacataa tcctctatac gttgcatatc taaagttatt
    47101 gaattataat aaaaatttac tgctataagt aaaaacattc ctactgaagt tagtaatagt
    47161 cctacatatt tcatagcatt tcctcctaag ttatatttgt tatcaataaa gtttattcat
    47221 caaataatac tttattacta catttataaa attttattat tttttataga ttccatattg
    47281 aaaaattctt gatattatat taaactaaca ttaaaattaa ttaaaactgc tttaggaggc
    47341 ttaaaatgtt tgagaaaata aaagaaataa tagcagaaca attaggggta gacaatttag
    47401 atgagataac tatggaagca tctttaatgg acgacttaga agctgattca ttagatgctg
    47461 ttgaaattat aatggccctt gaagatgaat ttggaataga aataccagac gaggaagctg
    47521 agaactttaa atgtattggt gatatatgta aatatatcga agaaaacaaa tagtatcgtt
    47581 ttgtctacaa atataaatag tctgtgtaaa cttctacata ataaattata ttagagttcg
    47641 cacagactag attacattta tttacaatat ataaaataca cgctcttata tttaatttat
    47701 atactaagta tataaattac ttttcttcat aaaacgtaac attctttatt attatatcta
    47761 tagttccatc ttgattttgt tttacagtat acttcattgg gtcttcaaaa tcagttagat
    47821 tcccttttat gtcaaaacca ttatccgttt ttatacttct ttttttaagc tttttctcaa
    47881 cccatttttt atctatacta aaactttcgt caagaccttt ttcttccata tgctctttaa
    47941 agctatcctt taactcatca tctttaattg aattatcaac aaagtcattt atatcaactt
    48001 catgtttttc cttcagtgta taatttaata tacttcttac atcttcagct tgctttatat
    48061 cattactaag agcattggtt atccaatttt cagctgtatt tttaaatacc ttggtcttgt
    48121 acttatcatc atctattttt ttagcattta aaaattctgt tacaaactta gattctgacc
    48181 cttctttttc agcatcctta tctaaaagcc ttagatgaaa ctcatcattt ataccactta
    48241 gactaatcaa agctccttgc ttttgtctta aagtctctgg gataccaatt tcatttgaaa
    48301 ccatttgaat attaaattta tcatccacaa attcaattga atgagtgtac aactttttat
    48361 aatctaattt cagtattgct acactctttt catctttaat agtgtataaa caaattgcca
    48421 aatcacaaga ttctagtgca gcattaattt tcataacgtc aaacaagtaa gatgcaattt
    48481 ctttagaatt tttcaaaaaa gtactttcat catatattat ttgttcacaa cagtttttaa
    48541 ttaaattgtc attataatct ttaaaaacac cttttcttaa atcctcatct tttgcaattc
    48601 tttttatagc tttctggaaa aaaccatcca cttcttgatt aactttgcct tcaaagtcat
    48661 ttaatattgg aacatcacta tttttgtcca aaacatgtat tataaattta tgtattatca
    48721 tatatatcac cccattatct taatttcgat attaactctt tcatatcctc tggtatatct
    48781 gctttaaatt tcaatttttc ttttgttctt ggttgttcaa actctagctt atatgcatga
    48841 agtgcttgcc tatttattag ctcttcatct acataaccat acaactcatc acctattatt
    48901 ccatgaccta tatgagccat atgtactctt atttgatgtg ttctaccagt ttctaagctt
    48961 acttctaata cagttgcatt ttctagtctt tctattactt tatagtgagt aacagatgac
    49021 tgtcctcttt catctattat cctctttatt gaatcttctg taggtctata aattggctca
    49081 tctatagttc cctcattttc ttttataatt ccatcaacta ctgttatata ctttttttca
    49141 actttatttt cactcattgc tgtagacaaa gtatgatgtg cataagcatt ttttgctact
    49201 ataactaacc ctgaagtatt catatctaac ctatttacaa atcttatttt tacgttttct
    49261 ttttgattat ctatatagta actaatacca tttgctattg ttttatcata gtggcttttt
    49321 gttgggtgaa ctaccataaa cggaggttta ttcaccataa ttatatcaaa atcatcataa
    49381 attatctgta aatttaaatc ttgaggttca aaatttgctt tttcctcgtc tatttttact
    49441 tctattaaat caccactata cacctttaaa ctcggttttt tatatactcc atttactaaa
    49501 acactctttt ccctcttcat ttttgataat gacctaactg agaaatttag cttgtccaat
    49561 aaaacttctt ttaaagtcat ttcttcttca tttgtatacg atataagatt atatctttga
    49621 ttctcttttt taaacaaact tttacctact ttcttttata tcttcctact ttttatatct
    49681 tcaaaatcat gatattatat attttacact atttttgttt aatagataat aattattttt
    49741 aactataaaa acctgtaaga ataacaaata gttattctta caggttaaat tcataattaa
    49801 atatttaatt tgtaaatatt ataatctaaa actatctctt aactattaaa gtagttccca
    49861 ttcctccacc tatacaaagt gtagcaagac cagttttagc atctcttctc ttcatttcat
    49921 ataaaagtgt agtaagtatt cttgctcctg agcatcctat tggatgtcct atagctattg
    49981 ctccaccatt aacattaact ttattcatat ctatatttaa gtctcttatt acagctacag
    50041 attgggcagc aaatgcctca ttagcttcaa ctaaatctat atcttcaata gtcatattag
    50101 cagcttctaa agctttttta gttgctggaa ctggtccata tcccattatt ttagggtcaa
    50161 caccagctgt tccataagaa actatagttg caagaggctc tattcctagt tcttcagctt
    50221 tttctttagc cattactact agcatagcag caccatcatt tattcctgat gcattaccag
    50281 cagtaactgt tccatctttt ttaaatgcag gtcttaactt agcaagtttc tccattgtag
    50341 tgccaggctt aatatattca tctttatcta ctacagtgtc accttttctt ccttttataa
    50401 caacaggaac tatttcttca tcaaattttc cttcagcttg agctttttca gctttatttt
    50461 gacttgcaag agctaattca tcttgttctt ctctagttat attccattgc tctgctatgt
    50521 tttcagcagt aatacccatg tgatagttat taaatatgtc tgataatcca tcttttatca
    50581 ttgaatcaac aaaagcagca tcacccattc ttgcaccata tctcgcactt ggtactaaat
    50641 aaggagacat actcatgttt tcagctccac caactaacat tatatcagca tcacctaatg
    50701 ctataagttg agatgccatt gaaacagatc ttaatccaga accacaaact atatttatag
    50761 tcatagctgg tttttctact ggtattcctg ctcctaatgc tatttgtctt gctatatttt
    50821 gtccaagacc tgctgtaagt actcccccta aaagagattc atctatcata tctggagtta
    50881 tgttagctct ttttatagct tctttagctg ctgttacccc taactctacc gctgaaactg
    50941 atttaaatgc tcctccaaaa cttcctactg ctgttctagc tgcactggca attactactt
    51001 ctctcatatt cattaccccc attaaattag atttttcata tatccgaaat tgttttttaa
    51061 acaattttat ttaaactttt aattcccaaa ttcttattta ttataatcat agaatcctat
    51121 cttagttttt cttcctaatt gattagctct aaccatttta gctaaaagtg gatgaggtct
    51181 atatttagta tctccaaatt cagtatataa aacgttcatt atagctaaaa caacatctaa
    51241 tccgattaaa tcacctaatg ctagtggtcc cattggatgg tttgctccta atttcatagc
    51301 ttcatctatt tcttctttac ttgcaacacc atctgcatat ataccaacag cttcatttat
    51361 cataggtata agtattctat ttactacaaa tccaggagat tcagatacat ctactggtac
    51421 tttattgata ctcttagata attcaaatac tgtatcaaaa gtaacttttg atgttaactg
    51481 accacttata acttcaacta atttcatcat aggaactgga ttaaagaaat gcattcctat
    51541 aactttatct ggtctcttag tagaagaagc tatttctgtt atagataatg atgaagtatt
    51601 tgttgccaag atagtatctt ctttacataa ttcatctagt aacttgaaaa catctttctt
    51661 tatattcatg tcttctacag atgcttctat tattaaatcc atatctttta aatcttcata
    51721 gttagtagtt gaactaacat gacttaatat ttctgctttt gtagcttcat ccatttttcc
    51781 cttagtaact aacttagtta aatttttatc taataaagct aaacatttat ctatagcacc
    51841 ttgagttcta ctctttaaac atacatcatg tccacaactt gcaaaagttt gtacaatacc
    51901 acttcccata gttccactac ctattacagc taatttcata gttataacct cctgttattt
    51961 tagtatttat attctgttaa ctagattaaa atactattta atctagttaa cagcaaatat
    52021 atttttaatt agatttttac ccttttataa agttagcttc tctcttttca acgaaagctg
    52081 acattccttc tttttggtct ttagttgaaa aacaaagacc aaataaatta gattctatat
    52141 ctattccagt atttatatca gtttgagcac caagttgtat tgcttcttta gagtatctaa
    52201 ctgcaagctg agcattttta gctattatct tagctaattt ctcaacttct tctattaaaa
    52261 tgtctggctc aacgactcta tttactagcc ctattttttc agcttcatca gcttttataa
    52321 cttgacctgt aaagattaat tcttttgctt ttgccattcc aaccaatctt gtaagtcttt
    52381 gagttcctcc atatcctgga gttattccaa gagttacttc tggctgacca aatttagctt
    52441 tagcagatgc aattcttata tcacatgcca ttgcaagttc acatcctcca cctaaagcaa
    52501 atccgtttac agcagctatc actacttttt tactattttc tatttctcca aaagcttttg
    52561 ctcctaagat actaaaatct ttagcagcta cagcatctaa atctttcatg tatgcaatat
    52621 ctgctccagc tacaaatgcc tttccttccc ctgtcaatat tacaacatca atagtttcat
    52681 cattattaat atctacaaat acttcataaa gttcttctaa agtctttgaa tttattgcat
    52741 taagggcttt aggtctattc attttcactg tacatatatt tccatctact tcaacagcta
    52801 cattctcata aactttaaca tcacttgtac tcataatacc ctccccaaga aattagttag
    52861 ctaaaacttc acctttttct tttgcaacac ttaactgact gataagttct ggaagcactt
    52921 tatgaacatc tccaactata ccaacatcag catatttaaa tattggagct tctggatttt
    52981 tatttatagc aactataaac tcagcatctt ccataccagc tatatgttgt attgctccag
    53041 atataccaca tgctatataa aggtctggtc ttacagtttt accagtttga ccaacttgtc
    53101 ttgctttatc taaccaacct gcatctatag tggcacgaga accagaaact tctccaccta
    53161 taatttcagc taattcataa agtatgtcta agttttcttt tccacccatt ccacgtccag
    53221 cagaaactaa tatcttagca tcttctattt taacttgttt tttagcttct tttattactt
    53281 gtacaacttg aactaattta tcagcatcat taaattctac cttgaaacgg ttaattacag
    53341 cttctttagt ttcatcaggt tcatttttct tcataacccc tggtctaact gtagacattt
    53401 gaggtctgaa atctttacaa actattgttg ccattatatt tccaccaaag gcaggtcttg
    53461 tcattaataa taattttgta tcttcagcta ctgcaagacc tgtacagtca gcagtaagac
    53521 ctgtatgtat tctagcagaa actctaggtg ctaaatctct acctattgaa gttgcaccaa
    53581 ataatacaac tatagggtca gctgctttta ttgcttcata agctgctttt gtatatggtt
    53641 cagttgtata cactgctaaa gcttcatcat ctactactat tacctcatct gcaccatagt
    53701 gtgctaatgt atctattaaa ccttctacct tactacctaa aagtaatgca gaaacttttg
    53761 tatcataatc ttttgctatt tctgtagcct ttcctagtaa ttctaaagaa acagtttgaa
    53821 ttacattttc tctttgttct attactacta aaacgttacc cattctttat ccccccttaa
    53881 attatatgat atacttctct tttaatttat ctatgataat tccagctgat gttttcgcat
    53941 cttcattgta tattgtacca gctggtttaa ctgattttgt aaatgattta aatacactag
    54001 ttggagaacc tttaagacct aaattagaag ggtcaacttc tatatctttt acagtccatg
    54061 tttctactac atcattttcg aaagcatcat atattcttcc aactttcatg tatcttggtg
    54121 tgttcatatc tttaagagtt gttataaggc atggcatttt aacttttaag tcatggcaac
    54181 aatcttcaaa ttgtcttttt actaatacat attcaccttc agtttttatt tcttcagcat
    54241 atgttattga tggaagattt aaatgttcag ctatttgagg tccaacttgt gcagtatctc
    54301 catctatcgc ctgtcttcca gctattataa tatcaaaatc tatatttttt aatgctcctg
    54361 ctaatgctga tgaagttgcc caagtatcag cacctgcaaa tgctctatct gttaataata
    54421 tacctctatc tgcacccatt gctaaagctt cttttaaagc catatctgct tgaggaggtc
    54481 ccattgttat aacagttaca tgagcaccca tttcttcttt taattttata gcttcttcta
    54541 aacctgcttt atcatcaggg tttattatac ttggtactcc atctctaatt aaagtacctg
    54601 tattaggatc tagtttaact tctgttgtat ctggaacttg ttttatacaa acgactatat
    54661 tcataaatcc ttcctccata tctattttaa tatcgaccta ttgtgtatat tttaatattt
    54721 atctatttta ataattttcc tgaaataacc attctttgaa cttcactagt tccttcatat
    54781 atttcagtta tcttagcatc tctcatcatt ctttctactg gatagtcacg agtgtatcca
    54841 tatcctccat gaagttgtac agcttttgta gtaacttcca tagctgtttc agctgcaaat
    54901 aattttgcca ttgctgcttc tactccataa ggttttccta agtctttatt tatagctgct
    54961 tgatatacaa ggtgtctagc cgcttgtacc ttaacttcca tatcagctaa ttggaattgt
    55021 gtattttgga attttgataa tggtctacca aattgtactc tttcttttac atatttaaca
    55081 gtttcatcaa gagcaccttg tgctaaacct aaagcttgtg cagctatacc aattctacca
    55141 ccatcaagag tagacattgc tatcttaaat ccttgacctt cttttccaag taaattttct
    55201 ttaggtattc tgcaatcctc aaatattaat tcactcgtag ctgaacctct tatacccatt
    55261 ttcttttctt taactccaaa gctaaaccca ggagttcctt tttcaactat aaatgctgat
    55321 attcctttgt tccccttaga tttatcagtc attgccatta ctacatatat gtcaccagct
    55381 attgcatttg ttataaatat ttttgagcca ttaagtatgt attcatcccc gtctaaaaca
    55441 gcagttgttt gttggccaga cgcatctgta ccagcattag gctcagtaag accaaatgct
    55501 cctaattttt ctccacttgc taatggtctt aagaattttt gtttttgttc ttcattacca
    55561 tattgatata taggccatga gccaagagat gtatgagctg ataatataac tcctgtagta
    55621 ccacaaactc tagacaattc ttcaactgcc attatatatc ctacagtgtc tccaccttct
    55681 ccaccatatt cttttggata tggtataccc atcattcctg cttttgccat tttttccact
    55741 gtttcataag gaaatctttc ttcttcatca agttctgttg ctaaaggttt aacttcattt
    55801 tcagcgaagc ttacatatag ctctttaagc atctgatatt ttttagaatt taaatccata
    55861 atagcctcct cagtatttta taaatatttt attttcattt attaattaaa atctcggcaa
    55921 catctataat aaggtatttg aatatacgtc gagaaattta taaccttaat caaactacgc
    55981 tctatattta tatgtgatta tactaaccaa ttatgacatt aatttaatca taaattgtta
    56041 atttaaaatc aactatactt aaaaaaaatc tcgctttttg gaattttgtg aaatttcaag
    56101 ttagaataag atttctttca cactggttag agataaaata atttgtttac gatatttata
    56161 ttaaggtata ttaaacatta aatattgtct gtacatctgt tttttagcca tgtacagaag
    56221 atttttatcg tataaaaatt attttaatag agctaaataa tcatattttt atctgactat
    56281 tttttatttt taaatatctt acatctataa tagtatcact caaaggataa tttttcaata
    56341 atttctaatt atttttttaa caatatttat tacatttttt ccactttttt gtcatttttt
    56401 tattgtttcg aaaaaaaatc agaatttttt aactttattt tggattatta gtttaaatta
    56461 gattttcctc ttagatttat aaatatttac acaaaaaaac tacctctgta tagattgtat
    56521 acagaggtag ttttttacta attcctacta tagtttacta tattttttta ccaattctta
    56581 caaacatcta cccatttttc acacttagaa aacttctcca tttcttcaca tgtaagttct
    56641 attgcagagt tactactgcc acatgcagga aatactgtat caaatctttt cattgaatca
    56701 tctaaataga cttttataga attttttaaa ccaaatggac agactccacc aatagcatgt
    56761 ccagtaaact ccaagacttc ttctggtgtc aacattttag ccttacaatt aaattcagcc
    56821 ttatacttct tattatcaat cttagcatca ccagctgtca caattaaaat tgcatcatct
    56881 cctattttaa atgagagtgt cttagctatt cttgctggaa taaccccagc tgcttcagct
    56941 gcaagttcca cagtagcact agattgttca aattctaata tactgtcttc cttgccaaat
    57001 tgtttaaaat actctcttac attacttatt gacattaaat acccctccta gtctgaactt
    57061 ttataaattt catattattc ttaattatat ctataattga tttatttttc aaattattcc
    57121 ataatattag cattccatgt atcattattt tttccaacta ctagataaag tccttttgcc
    57181 attggaatat tgacaatata ttctccttta tactctacac tcataccaga ttcattaaag
    57241 tacctataat caaaatttag tccttttgga attacaaaat caacataacc ttgattattt
    57301 ggttttattc catatttcag agttctttca gttatagaat ttttttcatt aaataaatct
    57361 acatttacat atgcagaaca cttattcatt ccctttttat tttccacaca attttcaaac
    57421 ttcattttag cactgtttaa tttataacta tatttactat tattgcttat tttaaatctt
    57481 acaatagtac caacatctct acttctcctg ctatcaaaat caaaaacaat ttcctctaaa
    57541 tgatttattt cataatcttt gacacttacc atatctctat atactaattt atttatagct
    57601 atgaatagtg taaaaatccc caaaataaaa aatgaaactt ttataaactt cttcattttt
    57661 agtctcctta tattttgtgc tattgtgcta taatattaat caaaatttta ataattaaac
    57721 ttattctttt ttgaaatctt aatagaaaaa tacattgata ttataaaaat tattattgtt
    57781 aaaaatgtca tactatataa atttatatac ttagtattag ctatatcagt tacataattt
    57841 gaaagatata ctcctccaac tttactataa ttaaaatgct ttacttgata tacaatcata
    57901 ctaatcatct tcaaaagact tccatataca aagtaaatta aaatacttac tataaaacca
    57961 caaactttca tttttccata tccaaactta aaaattaaag gcagtaaaat cgtcaaaatt
    58021 gctaaaaata tatttacaga aaaaaataca tcatttaaaa ccatatttct aaagaacact
    58081 agtgatgtca atttacttag taaggacaca attattgtta cagaaattat taatcctaca
    58141 cctaataaat attttgagta aactatatct tctatactaa ctggcatact ccttataaaa
    58201 atccttgaat cattttcata atcacactca aatgtattta taaatatcaa ataactaaca
    58261 acaatactta ctgtataata tgaaattgaa ttaaggaaaa aataaaacac aaaaaatagc
    58321 aatatgtact ttaaattacg gataccatct gaactaatcc ctattattaa atcttttctt
    58381 attaaattga acatcatctc acctcattta cataataata cattagttct tctatagtag
    58441 gatttttgac cttatgctta tcaatttcta tcaataggct ttttactttc aagctatctc
    58501 caactttaac aagtgcctca ttataatatt tatactcttt cacaccaact atgctttctt
    58561 taattgcttc taactctgct ttatttgccc taattatctt atattcttgt tctattaatt
    58621 ctttcttaac agtaaaaatc tgatttcctc tatttataaa aacaagataa tcagcaattt
    58681 taattaaatt atcagtatta tgagtagaaa ttattacact actacttcca tttttcacaa
    58741 aatcttgtaa gtactgcatt atttcagttc ttacaattgg gtctatccca gcagttggtt
    58801 catccaaaat caataactta gcattgtgac taagtgcact tgataacata agcttttcag
    58861 attgaccttt agataaattt tcaatatgca tattttcttc tatatcaaac ttattaaggt
    58921 attcatcaaa cctttctcta tcaaaattct tataaaataa agaaactatt tttctaaatt
    58981 ccttaacctt taaatgcgga taaaattgta aagaatcata tacaaaacca atattgtttt
    59041 taaagcttat tggagaattt tcaatagttt cattacaaag ttcaatttca ccttcatctg
    59101 catcaataat ccccattata atttttatta aagtcgtttt tcccgaacca tttggtccta
    59161 taatcccaac aatatcccct tcctttaact ctaagtttat attatttaac ttgaattttc
    59221 ctaatttttt tgtgatattc cttatgctaa acacatcttc acctcttatg cattttttta
    59281 tacccaccta tacatatttt aacacattca ctacaaaggt catttataaa tttataaatt
    59341 aagaattata aaactatgtt ataatacaaa gtatgaagag gtgaatatat gaaaagaatt
    59401 ataactataa atacaaacca attaaataag tcccttgaga ctaaagaact cttaactaga
    59461 aaacttatca atgctggctt tgaagtgtat tcagatattt atccagatac agagcttatt
    59521 atatcaattg gtggtgatgg ctctttttta agaacagtta gagatttcga ttttcctgaa
    59581 attcccataa tgggaataaa tacaggtcat ctggggtttt ttcctgatat tttaccagat
    59641 aagattgata gctttattga agcttataca aaaaaagatt atattattca agaaatgtca
    59701 cttcttaatg cggaagtata tacaactact agtggttcta atatgcttgc tgtaaatgag
    59761 gttgttataa gaggagacaa gtctaggact atacacctaa atttaagcct tgataataaa
    59821 catatacaaa attttagtgg agatggtatg attatttcta cttccacagg ttcaacagcc
    59881 tataactatt ctgctggagg tagtattgtt gatataaatc ttgaacttat gcagattaca
    59941 cctttacatc cgataaatac caatgcatat aggtgcttta catcaagtat aatatgttca
    60001 aatgaatctg ttataaagat tgcaccagaa tatagatttg aagattctgt attgattgtc
    60061 gttgatggag ttgagcacag atttagacaa atcgaaaata taaaagttag tatatctgat
    60121 gcaaaaataa aattacttag aatgtcaaac tatgagtttt ggcatagagt atctgaaaaa
    60181 tttttataga ctgtacttag gaggattttt aatatgaaaa taggaataat gagtgatact
    60241 catggtagct tactttactt tgaaaaagcg ttaaatgtat tatctgattg tgatgtttta
    60301 ttacatggtg gagatgtctt atatcatgga cctagaaatg atattccaga aggatataat
    60361 cctaaaaaat ttattgagac attaaataaa ttagaaaaca ttgtgattgt aaaagggaac
    60421 tgtgatgcag atgttgacca gatggttata gaacatccta ttcagagtcc ttatgtcatg
    60481 agccaatttg gtgaaattag aattatattg aatcatggtt atatagaatc agaagaagaa
    60541 attattgaca aagcaaagaa aatgggtggt gacatattag ttttaggaca tactcatgta
    60601 aaaaagctat acatggatga taatttaata gtaataaatc caggaagtac ttctatccca
    60661 aaagatggaa gccattctgt cgcaattata gatatcataa aaactgatga tgaggatgag
    60721 cttgaacttg atataaactt aattgacatc aatactggaa atataataaa tatcaatgat
    60781 tagtaaattt ttaataaaaa taaaataagc tttaatttat taaactcata ataaaaacaa
    60841 gtaaaccatt ttatttctat ctcaattaga agtttaagta caatacctta aacttctaat
    60901 taaatagaat ctggtttact tgttttatta tatgctatat tgaaatataa cttagtttac
    60961 ttatttcatt atatattata ctgaaatata acttagttta cttattttat tatatattat
    61021 attgaaacat agcttagttt acttatttta ttatatatat tgaagtgcaa cttggtttac
    61081 ttgctattat tatatattaa ttttgattta cacaatatta ctgtaaggct aatgcttctg
    61141 catatggttt actattttta cctccaccaa cttgtacaaa tttatcagcc tttacttttt
    61201 ttataaaact tttctgtgca ctacttaaac ttccacttgc aagtactaca ggtgactttg
    61261 tttttgatgc taatactcca gcagcaagcc catcaactaa atcagcttct ctaccacttc
    61321 catcttttgc aaaatacata ttattaacct tacttgattt tccataaaac ttttctaaaa
    61381 ctttcgcatt agtatctttt ctatctgtcc ctgctagtct ttctgagttt actaactgtc
    61441 cttcaagttt tttacttata ctgtactccc cacctactat gtatgtctta gttgagtttt
    61501 gtgtaataaa attctttgcc tcacttctta tgctatcctt acctgtatat attatagctg
    61561 tattatcact tgcagctggg cttgatattg aagttgcatc tgccagtcca ttatacccat
    61621 taaccactgc cacttttgag acacctaaat caccagtatc atctagctgt ctagtaagaa
    61681 gtaaagatgt ttcttctcta tctgctccac cgattctact gactaatact tccatatctt
    61741 caagagattc ttttacactt gatgaaatag ctccttcact tccaattaaa ataacttctg
    61801 ctggattaag cctttttatt tcctctgtta cattatatgg tatactattt ttatttgtaa
    61861 gcagtattgg tgcatccaat ttacttgcca aaggagttga agccaatgca tctggcaatg
    61921 aatttgagtt aactagaaac accctttctg aaccattatt ccaaccactt tgagatattt
    61981 ttatcgctgt atcaaatcta tcttcaccaa taagtttttc ctcctttgga gattttctac
    62041 cttgaagata attgttcata tctacctgtg cattttgatt tttactaata gtgtctgcat
    62101 atacacaact tggcaacata atcccaagtg aaagtgcaaa tgctattatt tttttagatt
    62161 taattttcaa tacaattccc ccttttaaat gttaatacct attgatattc atacttttta
    62221 ttgataccct aaactaaaaa taattatctt ttataaaaat aattacaaaa atagctatac
    62281 cattttaagt acaacacaag cttataatca gcttgactta taaagaatga tatagctatt
    62341 ataactttaa atgcttaatt tttaaatact ttttaactaa actctttcag catatttttc
    62401 agctattctt acaattagtt tagtagcttt ctccatagaa ctaactggta tacactcatt
    62461 tttcccatgg aagtttaatc caccagtaaa tatatttgga caaggtaatc cattaaaaga
    62521 taatcttgct ccatcagtac cacctcttac aggaactaaa cgtggtttta tacctaattc
    62581 ctccatagct tctttagcta tatcaactat aaacataact ggctcaatct tttctttcat
    62641 attgtaataa gagtctttta aatccaattc cactctatta tcgtatttct tatttatttt
    62701 ttctatattc tctatcataa tagattttct attttcaaat ttttctttac aatggtctct
    62761 aataatataa gccatactta cattctcgac atttccacct atagaattta aatgccaaaa
    62821 tccttcatat ccttctgttg tttctggtct ttcatctgct gggaacattt ctgatatttc
    62881 agcagctata tgtatagcat ttaccatttt atttttagct gaacctggat gaacatttct
    62941 accatgtata gtaatggttg catttgcagc attaaaattt tcacattcaa gttctccaac
    63001 tattcctcca tctaaagtat atgcatattt tgctccaaat ttttcaacat caaataaatc
    63061 agcacctcta ccaatttctt catctggtgt aaatcctatt tttatatctc catgcttaat
    63121 ttctggatga tttattagat actctattgc agtcactatc tcagctatac cagctttatc
    63181 atcagaccca agtagagttg ttccatcagt gactattaaa tcgtcacctt taaattcttc
    63241 caactctgga gaatccttta catacgtaat aacatttaac gcttcattta aaactatgtc
    63301 ttttccatca taattttcta ttattcttgg tttaatatct tttccactaa catcttcaat
    63361 tgtatctaaa tgtgatatga atcctatagt atccacacca tcagtatttc cttttaaagt
    63421 agccattata taactatttt catctaaact cacatcatca acacccattg tttttagctc
    63481 ttcaactata tacttagcta aaactctttg accttctgaa gatggacagt tctcacactg
    63541 agaattagat gttgtatcaa atgatacata ttttaaaaat ctttccacta ctttttgttt
    63601 cacgtatata ccctctttct taataagatt aatatttttt tatttacctg tgaataaaat
    63661 tttattttta aaaaatctat caattattat aattaaaaaa tatccacctt cttaagtata
    63721 tcatgcatac ataccaaacc caaacaaaaa attactccaa ttattccttt tacttggatt
    63781 ccaataaaaa tcgctgctag ggtagctaat ggatttagtc caacatttac tgatactagt
    63841 ttaggttcaa gtatttctct aatcaaagac aaaacaaaaa atatcacagt catacttata
    63901 gctataaaat aattttttat aaggaaataa taaacaatta ctggaagata tattgttatt
    63961 atacctagga atggaattaa atccattaaa gctcctacaa accctactat tagaccgtat
    64021 ttcaatccaa ataaagcaaa acttccccag attgcaaaaa atgttatagc cataagtatt
    64081 gtataggccc ttatatatcc aacaagtgaa agccctgcct ctttctttac gttttttacc
    64141 ttctttctaa catcaacagt aaacatatta taaaaactat tttcaatctt atcaaggtct
    64201 tttgctataa agtacgtagc aataaacaag gtaattataa atattataat atatggaata
    64261 gaagtagcca atccaagtat actagtaaga agattttttg taatttgcat tacttcacca
    64321 ctgaatttgt ttactaattg ttctacatca aaattggcta tttcttgaaa tgatttcata
    64381 aagtcattta ttttatttaa aaaatacata atagtattac ttatatcctc aggattagca
    64441 gatatattat ttaaaaatcc cattaattct ttcattgaat ttattactat tattgtagta
    64501 actgtactga ctatagccac tgcaagaaaa cttagaacaa gagtagagat acctttattt
    64561 atacgaaact tattttctaa cttttgtgat ataggattaa tcataagtgc tattatagca
    64621 cctagaaaaa atggagctat gtatggaaac gctttgtaaa taaaaataaa taacaatgta
    64681 tatatacaaa aaaagatagt gttgtttttt aatttgtata aaaaattatt ttctagtatg
    64741 ctcatcttaa aaatctcctt tcttctaatt tatattttta ttttgtccat ttcatactag
    64801 ttttatttga atacaaaata aaaagaagtg tataaatata aaagatatct tttatattta
    64861 tacacttcta ataaatttaa gaacattatt acattatgta tatattctta tttttttaat
    64921 tctatttttt tctatacttt ctatacaaaa tcttatatta tcaacctcaa ttacttcatt
    64981 ttcttctggc aatctgctta ggtgtccaat tataaaacct cctatggaat caaactcctc
    65041 agattccaag tttacaccaa taagttcatt aacatcacca attttagtac ttccatctac
    65101 aatatactca tcttctttta taacttgtat ttcatcctct tcttcatcat attcatcctc
    65161 tatatctcca acaatgactt ctactaaatc ttctatagtt aaaagtccag atgttccacc
    65221 atactcatct acaactatag ccatctgact tttttcaagt ttcatctctt caagaagttg
    65281 tgttatcttt ttgaactcat acgtgaaaaa aggttctctc atataatttt taatatcaaa
    65341 tgactctatt tcttcatcag ataaaaatat aatatctttt atattaagta ttcctacaat
    65401 atcatctata gtttcttcat acacaggcat tctgcttaat ttttcagttt taaatacctg
    65461 tattatctca tcataactat cttccatatc tatagctacc atatctattc tttgtaccat
    65521 tgcattttta gcttgcatat ctccaaattc aaaaacatta tttatgattt ctctctcttc
    65581 catttcaagg acgccttctt catgacttac atttaccata gtttttagct cttcttcagt
    65641 tatgaatgat ttagctcctt tatttgttat accaaataat ttaaatataa tatttgtaat
    65701 tatattaaat atccatacta ttggtcttaa tataaatata attgctttta taggtttaga
    65761 tactaataga gaaacctttt ctgaattatt tgctgctatt gtttttggag taatctcacc
    65821 aaatattaat actaatactg tcataacagc tgtagcaagt gccactcctt tttctcccat
    65881 taatcctata aacaaagatg ttgatatgga tgttgctgct atatttacga cgttatttcc
    65941 aaccaatata gaacttaaaa gcttatttgg attttctatt aatgaactta ctaacttagc
    66001 tcctttaact ccttcatctt gcatatatct gattctgatt ttactcaacg acattaaagc
    66061 cgtttcagat gctgagaaaa atgcagaccc tataagtaat actattaaaa aaataatctg
    66121 aatcaaatta ttgggcgatt ccaacactta ccacactcct acttacatgt ttttatatta
    66181 atattaatta taaataactt taatattagt ttttattata tcacaattac atattatttg
    66241 gaaatattag ataatatatt caattaacat atattttcta actatattca atttttactt
    66301 ttgaacctat tccagcctca ctagatgaaa ctaatagctt aacaggaact ctatttttaa
    66361 gttcctcgac atgacttatt atacccacac ttagattatt actatgcaat ctttccaatg
    66421 attctataac aatttctaaa agttcactat ctaatgaacc aaaaccttca tctaaaaaga
    66481 aaaattctaa tggtgcactt ccctttaatt gtatttgaga tgaaagagca agtgctaatg
    66541 atagagatgt caagaatgtc tctccaccag atagagtatc aatgcttctt ctttctcctc
    66601 cattaaagtt atctctcatt acaaagttta gtgttgagtc aatttcaagt gcatatcttc
    66661 cttttgttat tccatccaat cgttttgatg cttccaacgc tatatatttt aattggtttg
    66721 tagaaacata ttctacaaat ttatttcctt gtacacattt atctaattct tccaataagt
    66781 ccacattgtg ttcaactacc ttaagttgtt tattaaaatc atttatctta tctaaagagt
    66841 cttttaatgt tattaactga ttttgattag ctccaatatc tttggatata tttcctattt
    66901 ctactttcaa attataaatt tcatctttta actgttcaaa gtactccttc tttatgcttc
    66961 ttccatttaa tttttctttt gatgatttta ttttaaatga taataacttt tgttcttctt
    67021 catgctctag tatttcttca atcaattttt ttactgcatc agtatctaat aaagctcttt
    67081 ttactgcata tatactttca aatttatttt ctgctaatag tttatttagt gttgattttt
    67141 ggtctttata ttgttcctta gcagttttaa gtctaccatc tatattgtgc ttttcagcaa
    67201 tgtttttttc atattctaat ctttgctctt ccaatttttt cttacttgat tcttcttgtt
    67261 ctaatatctt acatatattt tcttcaacat tatgtaaaag atttttagct aaatcacctt
    67321 tcgttatgct aataacttct ttatacttat tatctctact caatttcttt tctacatata
    67381 attctctagc ctttattaat tctatctcta tttcatgtag tttactctca ttttctctta
    67441 ttttaatatc tagtgagtct ctatttttta ataaatttga atgcccagta cttagtaatt
    67501 ctaattcttt ttcattctct cttatttctt ttacttttga caatagatta gagactttag
    67561 taatcgtctt taatcctagg tattcctgtt taagtttttt acatttattt tctaaacctt
    67621 ctatttctat gtttaaatct cttgttaatt tcttataatt attcagactc tctcttattt
    67681 ttaattcttc tttttcaatt ttattttttt cttctttagc taatgttact ttattttcag
    67741 tactctcttt ttctttctcc cactcctgaa tattagattt taacaatgca agttttcttt
    67801 gttcttcatc aagttttttt aataattgac tagatggaat cccccctagt tttcctttta
    67861 catcttcaag ttctttagtc ttcatttttt ctatagataa atacccactt actttagcat
    67921 ttaattcctc tatattgttt cttatactta ttttttcttt ttctaatttc tccagttttt
    67981 ctttgacaaa agatatattt tcatcatact tagttatatc atgattttca tgatgttttg
    68041 acccacaaac tggacaaggc atattttctt ttaattcacg tctaagttca gaggctaaat
    68101 ttaaatactt tagttcttct aattcttttt ctaagtcatc tctgttcttt ctattatttt
    68161 caagtttttc atttagtaaa tttacttccc tttcagtatt aaacttactt tcaagtatta
    68221 tctttaattc atcttgtata ctgcttttct taatttcatt ttctttagta ttatttgctt
    68281 tatttcttaa ttcagtaaca tattcagatt ttaaaagtaa atcagcactc ttaccaggac
    68341 attttttaag aagaacatcc aagtgaagtg ataaattttc taaattacga tttacatcat
    68401 tcttatcttt atcaatatat ctaactttta aatttatatt ttctgtctct ttgagtattt
    68461 cttctagttt atttagcttt tgattttttt cttccaatac gttgttgtaa tctttctcgt
    68521 attcatatgc tagaaatatt ttttgtttta attctgcact tatatttact ttatcaatct
    68581 ttccttcaac ttcttttata gatttagtaa ctgcatcttt tctggactct gacacctgct
    68641 ttactttttc taattcaact ttagtcttat ttaaattaat tccactttct tttagctctt
    68701 ttaactctct atctaataaa actaactctt cttctaattt tattgcttgt tgcaatctta
    68761 ttttttcttc actcaattta gggacttctt cattcttaat tttgtttatt ttttcatatc
    68821 tattttttgt aacttcaagc tcctgattta atattgctaa tttcttttct aaattactta
    68881 ctgcgaagct atcttcatct atcttttttt ctagattttg aactgaactt atgtatgggt
    68941 ctaccttttt agcgttacta tcattttcta caagagcaat cttttctttt atctcactat
    69001 tttttaaatc aagctcattt tttctaagtt catttttttc taactttaat tgttcttcat
    69061 atactgtttt actttcttca taagattttt gagctaggtc taaagcatta tccttatctt
    69121 tttccaaatt ttttaactct attaattctt ggtttatatt gttgtatact tcctctgtaa
    69181 ttccatcata ttgacttaat tttgatttca aatcttttaa attttgtaac tgtatatttt
    69241 ttctcttttt cactttttct ccaagacttc ttccatattt ctcaagatta aaaattctct
    69301 ctaacatgtc tcttctatct gcacgtgata atcttagaaa ctcattaaat ttgtcttgtg
    69361 gtaaaactac tgacctagta aaatcatttg ctgttaatcc tacaacctgt gctacttttt
    69421 catttacttc cacaacttta tcagctaaaa cattttgggt attgtcattc aatacttcta
    69481 ttaatctagc attagaggtc tttgttcctg ttttgcttct agcaatggtt ctatctacaa
    69541 tgtatcttct tctagtgttt ttgcttccta tctcaaactc gtatgatata attgccttat
    69601 cgcacacact gtttatatat tcttttgtat ttctggatat atttccatac atagcaatag
    69661 ttattgcatc taagattgta gattttccac ttccagtagt tccaaatatc ccaaacaaac
    69721 ctctttcaat taatttttca aagtctataa cttgtttatc aatatagcta ttaagccctg
    69781 taagttctaa tctaattggt ctcatctgct gattcacctt cttcacttat tatatctaga
    69841 aataaatcca taagttctcc ttttggctct acacctttac taaaagaata gaactctcta
    69901 aacaactctg ccatagactt ttcttttata tctacacttt cttgttcata acaagatgtt
    69961 attattggtt ttatttctat tatgtctttt aatagttcct tcattttttt aatttcattc
    70021 tgtgatatta tctcatcagt atttatttca aaatatgacc aaatctctct atcttgattt
    70081 tgctcacata tatctagtgc ttcttctatt ccattacatt taaatacttc tataggtttg
    70141 taattattaa aatatacatc ttcaattatt ggtttttcac cagcttttat atccactatg
    70201 tatgcccctt tggtatacgc tctctcatcc ttgctatatt gaagtggaga accagaatag
    70261 tatgcattta aacgttcgga agctttttgt tgtttatgta aatgacctag tgcagtatat
    70321 tgagcctttt ctggcaaatc ttttctttca actaaaaaac ttccacctaa ctgtataggt
    70381 ctttctgatt cagtactctc tccacctatt acaaatatat gtgatacggc tatatttata
    70441 gtatcacttc taaaattttc ttctaaactt ctaaatatat ctcctacctt ttcagaataa
    70501 tttttttgtt tctcaaactc atcttcactt gaaaaaactt catttaatct tttttcactt
    70561 ggataaggta atgtagcaac cacaatattt tctccattta tattcagttt tgtacatccc
    70621 tgagttgcat atgttatttc aaacccttta tacttagctt ctatcgtagc acttaatgga
    70681 taaccatata taagtatccc ctgttcatgt gctagaggtg ttattgcaga gagtctctct
    70741 ggattatcat gatttcctga aattattaat acacacctct gaccattatt ggcaagtctt
    70801 gatacagtct gataaaatag tttttctgct tgtgctgggg gattagaagt atcatataca
    70861 tctccagcaa ttataaccat atctatttca tttgattcta ctatttttat aaattcctca
    70921 caaaactttg cttgttcctc aattctagaa tggccttcta gggacttccc tagatgccaa
    70981 tctgatgtat gaataaatct cattttttcc ccttcctcta tcttttaggt ttaatttata
    71041 tataaaaact aataacaaac agcttcatca actccaaata aatatataca tttctctttt
    71101 actgacttac cagttaaagt ttcaataata tctgcatata aatccaactg ttttttatat
    71161 ttctctatta tttgatttat attttcctca tttacaaaat ctgttttata atccaccaaa
    71221 actatttgat tatcttcttc aaaataagca tcaactatac ctcttaacat gacactttca
    71281 ttatcataaa gttttttatc gtcattgttt ataagctttt cataaatata tatgtctttc
    71341 atatttactt gtgcataaat tgatttttct ctatttataa tttcagcatt caacattctt
    71401 tttcctatgt tagaggcaaa aaatttatat attttatatg gatttactat actagcttgt
    71461 ttttctgtta ttataccttt tgaaacaaaa cctcttattt gtgattttat atcatttaca
    71521 ctacttacat tttttaagtc taaaacctcc ataactaaat ggactattgt acctctttct
    71581 gttccactta ttttttctct ttcttcttca ttttgaataa atagaggctt ttttaatatt
    71641 actttttgtt caaatatagt atttattaac tcttcttcat aattattttg tatctttttt
    71701 atttctgtaa cagatatagt agcaggtttt cttgtactaa attcatatgg atatatatag
    71761 tttaattttt cttctatttc accgtaataa tcactgtcag gattatttac atctattttt
    71821 tcaagaattt cttcaatact ttctttttct tcatcactct tcttttccac taaaatatca
    71881 ttcttattcc acaactttcc ataccatttt gaattatgtt caacattaaa aactgcgtct
    71941 aatcctactt cttctagtag attagataaa tccctatgtc ttaatacaca aggcattatc
    72001 caatctaaaa aatttttacc ttttaatatc tcgtattgtg atattgtatc taaactttca
    72061 attccatttg accatctctt tatactatct tgtatatttc tagtagaacc agttataatc
    72121 aatttttctt tagctcttgt aaatgcaaca tataaaactc tcatctcttc tgataaattt
    72181 tctatattta ttttgctttt taatgcttct ttggctatac ttggaaaaga aatccttctc
    72241 tcatagtcta caaattgagg tccataccct aaattatgat gatataaaat actcttttta
    72301 aaatcttgag tattgaaatt tttacccatt gcagagcata tgactactgg aaattctaga
    72361 cctttacttt tatgaatact cattattcta acaacatttg cattctcacc aagtgttttt
    72421 gcacttccca tatcagaact agattttttt aatttctcta taaaatttac aaagttgaat
    72481 attcctttaa gactggtttc ttcaaattgt tttgctctct caaacaatac ttttaaatta
    72541 gcttgtcttt gacttccacc tggcaatgct ccaacatagg catagtatcc agtccttgtg
    72601 tacaagtacc atataaattc atctgtactc atgtacattg acttttcttt aaattccttt
    72661 agcttaatta aaaaatcttt acttttgttt atgcactctt cacttggtat aaactcagac
    72721 tcattttcat tttgagaatc tgtaaatcca tcatactctg ctgtactttt taaaacctca
    72781 taaaaaattt tatctttact ctgaactcta atatcaatca aatcctctgg tgtaaatcca
    72841 aatataggtg attttaatac agatataaga ggtatatctt gcattggatt atctattatt
    72901 tgaagtaatg acaatattgt ttttatttca atcgtatcaa aataccccac tccaacatca
    72961 gcatacgttg gtatatccat attcatcaat tcatctgcaa acactggagc ccatgcagat
    73021 gtagctctta aaagaataac tatatctcta aattccacag gtctatatcc atctattccc
    73081 ttatcataga ccttttgaat ttttccatct tcattgactt tcattaaatc tttgataatg
    73141 tttccaacca ttctagcctc aagttgtata ttatctaatt tttcttcttc ctctatttca
    73201 ttttctttgt tattagttct atcgtcttta tcatttataa tatcatcatc aagtttatta
    73261 tctttttgta ttaggtgtat ctccgttgct ccaccaataa tagatttctc gtctgtatcc
    73321 acattaaaat ttgcacctaa atttagtctt tctttttctg tatattcaat ttctcctata
    73381 ttctcattca ttatattttc aaatatataa tttacagcat ctacaacttc ttctctactt
    73441 ctaaagtttt tgtaaagcat tatttttcta tgggagcttc cctttttatc attatagtta
    73501 ttatattttt gcaaaaataa ttctggcttt gcttgtctaa atctatatat actctgtttt
    73561 acatcaccaa ccataaatct atttggagtt tctgtatttg caacagcttt aagcagtact
    73621 tcctgcacta gattactatc ctgatattca tctataaata tttcataaaa cttgtttcta
    73681 catccaactg ctatatcaga aggtacaata ttaccttttt catctacatc tgtaagtata
    73741 tttaatgcaa aatgctctat atcgttaaaa tctattattc ctttttctct tttcttattg
    73801 ctatattcct cttcaaatct cagtacaaca ctagatattg gtttaactat gttataaaga
    73861 tattttattt cttctctaat agaatcatta tctttattga atgtagcact tactatactt
    73921 tctaaagact tcttagtctt atctcttata gtttttgcct tttcttttga ttcttttata
    73981 tacgaaggag catcttttga aattcttttt actcctttta cataattttc aaaggacata
    74041 cttgccatct ttttatatgc ctcatcccaa gatttattac atgcttgtga aatgtcagca
    74101 atttttttat attcaacgga taacttttct gcaaaagttt ctaattcttc tattgattcc
    74161 acttctttta atgccctttc catatttaga caaagaccat taatctctat ttttacagtg
    74221 tctaatatag ctctagccca aatagactgt gaaaaatcaa aattttcatc aatattaaat
    74281 cgttctgctg agtcaatcaa ccatttttta ggttcaggag aagccattgc aaatgaatat
    74341 attcccaaga taatatcttg tacttcttta tctcctcctc tttctgcata actttcaact
    74401 aaatttaaaa acccctcatc tctttcttca tataaatctt caaatacttc ctctattgct
    74461 tcttgtttca aaatagcaca ttcagtttgg tctcctattc tgaaattagg gtctaagttt
    74521 attctatgaa agtttgattt tataacatcc aaacagaatg agtgtattgt tgtaatacta
    74581 gatttattta ataaaacaag ttggttttgt aaatgtttat tttctgggtt ttcatctaag
    74641 gcttttccta tagcatcccc tatcctctct ctcatttctg acgcagcagc attagtaaat
    74701 gtaacaacta ataacttatc tatatcaatt ggattttctc tacttgttat catttgaatt
    74761 atacgctcaa ctagaacggc tgtttttcct gaaccagcag cagcagcaac caataaattg
    74821 cattctctac tttctataac ttctaattgc tcttttgtcc atttaggaga actcactaat
    74881 taacatctcc tttcatcatt tttattatct cttcattact cttattattt aaatttttat
    74941 acttgttatc ttttatagtt gaatcaaact gacatatagc agagtaatca caaaagtcac
    75001 atgatgttcc atctttatgt ttatatggag ctatacgtat ttctccactt aacatttctt
    75061 cacataaatc ctttatagca tgccttacat attttctaag cagtttgaaa tcctcatagc
    75121 ttattgctga agtactttta cctacatttc catccttagt aagacctact ggtattacta
    75181 aagagttttt cctttctcca tctattaatg atttatccat ctctttaact atgtgagagt
    75241 cttttactac aagccccttc attctaagtt cttttaatat tgcttcttgt atttcttcat
    75301 catctttatc ttcattaaat tttgctattg gattatttat tctacaatat agtattgcag
    75361 ctggatttat atttatatct tcatcctttg cactttcaag gattgcatct aaatacacaa
    75421 gtaattgtaa ttgtagacca taatatattt ctgttagact tatggactta ttgcctgact
    75481 tataatctat tattcttata tatttatttt ctccttcttc aaattcatca actctatcta
    75541 tttgtccaat taaacttact tcttgtccat tttctaacac catttttata ggtggatatt
    75601 ttccattgtt gccaaattta acttcataat ctattggttc aaatgaacct tgttttattt
    75661 gctgactaat tatagttata gcagttgtca acatattctt taatctatat gcaaggtatt
    75721 tatacctctc agaactattt aatatatatc ctggtatctt taaaattatt tcatcaacta
    75781 ttatacctat ctttaattct atatattttt catcaatatc tctccaagtt aaattatctt
    75841 gtaaaagttc ttttgaaaac ctatctaaaa tattatgaat aaaagttcct aaatctggtg
    75901 gtgtaaatga atactcttta cgcttttttg cttttagacc atattgtata aagtaagcaa
    75961 atggacattc agcatatttt tcaagcctag atatgctaag agaattactt ttatacaatg
    76021 accttatttt tttctcttca atcttatgaa cttgattagt ataactaaga ccttttataa
    76081 ctttttttgt tatagaacta tagatttcat cttttaaata gtacctatat atatctaacc
    76141 aaatactatt aatctcttct gtattgtaac catcactgtc ataatttttt attacgttta
    76201 taagttcatt aaatgtaggg gatttaaccg ttattttttt aagtatatct ttatcagtgc
    76261 ttttattttc ttccactaaa taacttttat tttctatatt tggaaatatc ttcttaagtc
    76321 ttgatataat tattgatggt ctaagcgttt taccttcatg gtctgatatt ggatatgtta
    76381 tggttaaatt cttagatgtc gaagtaagag ctttatatac aagaaactgt tcttcaaaac
    76441 tcctagtctt actatcaata tcaacttcga ttcctttgtt tccaagactt tccctatcat
    76501 tatcactcaa tattccacta tcctttgtaa tcagcggaaa cacaccatca gtagttccaa
    76561 ctaggtataa atatttcgtg tctggatttt tcattctatc cacactactt acaagtacct
    76621 ggtctatact aggaggaacc agtcctaatt catattcatc aaaacctaag gttattaatt
    76681 ttataaattt ttctaaagaa attatttcat cacccataag ttccaccatt tggtctaata
    76741 tgtccactac tatgtcccaa acttgtgagt attgatttgc aacatcaagt tcccctttat
    76801 ccttaaaatt tactattaaa ctttctatag tttctggcat atttatatct aataaaaatt
    76861 catatatata tctacagatt tcttttactc tattttttcc tcttaatttt tcttgtaaaa
    76921 ttactatagg ctctaatact ctatttttta tttcatttat tttctctttt aattccagtt
    76981 caaattcact ttcttgacct gaaagacttt gggttattct ataatcccat ttttcatcaa
    77041 accatttttt acctttaatc ccattagcca acacataatt ttctagtaaa tttatatcat
    77101 cattatcaat tcctataagt ccacttttta aatacctaaa catagtttca tacccatatc
    77161 gtctattctt catttctaaa gctgaaataa ttaaaataac tattggatta ctttttgcct
    77221 ctctcttctt atctataaag tttggtatat tatattcatt aaatatagag tgtactaaaa
    77281 aatcatatct atttaaatct cttgtagcaa tggttatatc tctatatctg acattcttat
    77341 ctctaactaa atgtacaatt tctctggcta tctcttccac ctctgaatat aaattgttaa
    77401 actcttttat ctttatattt tcagttggat ttgaatatat tttatatgga taagcattat
    77461 aaaatctttc taagtgctgt aattccttaa ctttttcaaa tctttttata acacctgtgt
    77521 ttaaatttac ttgtggcaat atttttatgc cttcttcgtt gcacaattga gtcaattttg
    77581 aataagtaaa tttcgttcta gaaaacatat cagatttact gtacccaata tagttgatat
    77641 catctacagt caaagagata ttaacacttt tagacttatt taatatactc tttatgacat
    77701 tgtactgatt tggtgtaaaa cctgtaaatt catctatata aacacaagct ccatctaaat
    77761 aactgcttaa ttcaatttta cttgccaatg aagttaacat atcttgagca tctacataat
    77821 tttcatgcaa tttcccttca aaagaattat atattttact tatatctttt aattttaaac
    77881 ttaaagtttc attatccaat tctccagata tattttctaa catttcaggg cttatattgt
    77941 attgtttcat ttctgatatc atatctgtaa ttgaacttac aaaaccagac tgcgattttg
    78001 attttgaaaa cacatttaat tcttcactta catcttcaat agctctatat accatcattg
    78061 cttttccact agaatttata tttacatctg tcaatcctcc tacttgagaa aaaactatat
    78121 tacttagtgt cttaaagcta agtaccctaa ctcttagata cttatctttt tcttttccta
    78181 aaaatagttt acttactcta ttttcagcct caaatgtgta ctgttctgga actaaaagta
    78241 taattgatgt cgtttcattt ttttgagctt cttttttaat ttcatctagt atataggtgg
    78301 attttccact tccacttcta cctaatacaa acctaagtcc cataaatttc cgcctccaaa
    78361 atactataat ctatttcttt gccattacat aacttattaa attcacaata cttgcaatgc
    78421 tttttatttc tatagcacat acgttcttct ttattatata ccttttctat atcattgtat
    78481 agattgtctt cgtatgtatg ttcttcataa ttagtgtttt ttatcatact tatataattt
    78541 tgaattttat ttctatttgc ttcatgtttt tcttcatcat ataaaatggt aattggtaag
    78601 tcatcaaatt caggttgata atatctcata tttatatctt taacatttat gtccattttt
    78661 aaaactttag gtattacttc ttttgctaaa aacatataaa caatagtttg tattctattt
    78721 tctacgtgtt tatagtcaat ctttttagac tcggttttcc aatcccatat ttctatattg
    78781 ttatttccaa ttacaactaa atcatacttt gcctgtatta tatccccatt taatgtcatc
    78841 cttacttcat attctggcaa atacgttttt cctttttcaa taggaacaac tttttttata
    78901 ttttccatcc actttaaaaa ctttttacta tttttatcat cctcattaaa atagattcca
    78961 agaggtatat tgctaaaata tctttcacaa atcaaatgaa aatctcttcc tgtttttaaa
    79021 gtttcataat actctctact accaacatca tcatttttcc aatttattct atcaatatat
    79081 ttatatttaa atttcaatgg acatgattta tatgtgttaa ttgaattttg actataagaa
    79141 aaatatttta atttatcgtt cactcatatc acttctctct taactttatt ttttagtagt
    79201 tacttactgt acatattgtt ttatcccctt ttagatatat ttcaagcaat cttttaataa
    79261 cttattatat attaatttga ccttttttca ataatatgtt gattaagtct gcttaaatat
    79321 atacatggtt tttgctcttt tctcttattc cctatatcac tttcatctct ataagcactt
    79381 cctgaaagta ctaacatttc ctttgctcta gtaatcccta catataaaag tctaatcttt
    79441 tcatttatag aatcaatctt cgttttatga gcataatctg ttgaaatact tccttttaat
    79501 atagcttcaa cttctgattt aataattgcc atagggtttt tatatttttc ttttagatac
    79561 catttatcac tctgaaactt ttgattaata ttgtcaggga aattatattc aacaagacct
    79621 agtaaaaaca cacaatccca ttccatcccc tttgatttat gatagttaca tagtgttata
    79681 ctaccgggct ctggttcata tccatttatc tcatatacca catctattat atgattaaac
    79741 accctgttct ttacatcaaa caataaatta tatacatctt ctaaatccat atttatattt
    79801 tctgatacta gatactttat ataaaaagat atataatcca gtacagcttt atcttctttt
    79861 tctaaattaa gttttttccc aataaacaaa actaagaggt ctaaccttat tattggatat
    79921 tctaatatct gttttataat atctattcct cttttaaaac ttaaatatat gtcactttct
    79981 tctttaaatt ctattaaatc attatctaac ttactaaatt caaaattata aataatttct
    80041 tcagtagaat agttttttaa ttcttctaaa aaatacttta tttcttcttc attatcagta
    80101 tctataaaaa ctttattaag tacaactatt aacttttcta catcatcaca atgtaacata
    80161 aaatcaataa tatatgctat gttgtttata accttccttt tattcaaaga atttggtcct
    80221 aattcttcaa aagttagatt attctctcta agttctttag caacttgtgt cacttgttca
    80281 ttaaatggaa ccaatatacc tatactttta tctggatatt tattttttat acctttaaca
    80341 tatctagcag tttgctctat ttcaattttc caactctcat accactttac atttatatta
    80401 taatgttctg gattaggatt ttctttatat cccattccat ttggcacagt ttttatttgc
    80461 atatcttcaa gagcatgtct acattctttc tgtctaaatt cctttgtaac atacttgaca
    80521 agagtgtttg ctaaatcaag tatatctttt gaacttcgat ttgacatatc catcctataa
    80581 cagaaatctg cactatttat aaattcttta aaaaatttag ggtctgatga tgaaaatgtt
    80641 cctgttatac tctgatttat atctccaact ctaactaggt ttttattttc gctagatata
    80701 atttttatta tattaccttg aatttcattt gagtcctgac actcatcttc aaatatatat
    80761 ttgtatctag tttgaaactt ctttctaagc ccttcgtcta agcttaaagt tttttctgct
    80821 aaaattaata tatcatcata atctagcaat ccattttgct ttaattttct atcatacaat
    80881 ttatatattg gtaaaataat tttaagcatt cctttgtgtc ctcttgacaa tatttcttca
    80941 aggttatctg gagaaattcc cttatactta agttcactta ttgcatttcc tgcaaattca
    81001 aaaaaacctc tttcccaggc ttcaagagtt atttctttcc agcgttcatc tttttgctcc
    81061 tttaaaaacc atctaaaagc tctttctcca ccttcaaatc taaatctact tatacattca
    81121 tttaatataa tacttttttg caaatcgtca gctatgttaa aatcctcact taacattaca
    81181 acttctggct tttcctttat tatttttaca gccaaactgt gtatagtcat tacttcatac
    81241 gaattagtat catctatttt attctcttca agaatttttc ttattctacc tttaaaatta
    81301 tttacagcac tattcatata tgtaagaatt aatattttac cacccttatg tttctgctca
    81361 agtaatagtt tagctactaa atttgttatt ataaatgttt ttccagcacc tggtactgct
    81421 ggtactgcca tagtaccatt atcgtaattt attattggta tctggtcttc tctataattt
    81481 atattaatca taatttttat ctcctttatg tgagattttt aaaagtatac tatataatat
    81541 actctcttgg atataaccat taatcgcata ttcactttta aatgcataga ttttctttgc
    81601 atttaataac aaattatata ctgtattgta tagatagtat tttttataat attcttccat
    81661 ttcatttgta taaatctttt tttcttcaaa agattttcta agtacaatta aattacttat
    81721 atctttttct attttcatat tccaagcatt acttcctata tctacccata tttgtatact
    81781 tctatctata tttgatgata tatatgaata aggagtagtt attactattt tgtctttgga
    81841 tttcaactct tctatatcga caacagagta ataatcgttt attgtacttt ttaaagcttc
    81901 tataaatatc ttttcttttt tcatcccaag tagacttaaa ctttcactaa aagactcact
    81961 ttcagctatt atctttttac ataacccaac attcttttta ccttctttta aatttaacat
    82021 tttatctatg taaaattgag ttaaaaattc atgtattttt atatcactct tctttttttc
    82081 ttcaatatac tgtaaaatct cacgatactg actatgttca tctatattat caatacttaa
    82141 atgtcttgtt tcattaaaaa tcttaaatgc ttttatccta tttatttcta aaagagtttc
    82201 taaaaagctt aagtattcat catcttttat aaaatctaaa tatccataaa atatacatgt
    82261 agccactacc aatgcattcc catatggata atccacagct tttttatcct tcttagtact
    82321 aaaaacatct atattttttt ctatcaaaga atctctaatt tgatgctcca atatactatt
    82381 gtttatagga gatataattg ctatatctct tggagataca tcttcttcta taagagatat
    82441 aactttttca cttattaaat ctaacatttc attatataat tgacttgatt gatttaattc
    82501 tatatttgca ggtaataagt ataaatcttc tattctaatc tccttctttt taatattttc
    82561 ttctttaggc ttaatttttg atattatttt ctcatttata tagtctatat ccacattatt
    82621 aaatacagaa taatccctac tataatcaaa atatatataa ctttgtaatg tattatccaa
    82681 tgcttctgtt ataaaatcaa cttctgcatt acttgagctt tcaaggctat ccacaattat
    82741 atatctcact tctgtaaata agttttttaa ataaaaatca tcattaagta ggtattgatt
    82801 gtataaatat atgcttaatg cattatctaa aattgaattt gatagtaagg aatttatata
    82861 atatgatata atttcatcca tttgagtgta tgaaaatcta tatatactat ctttattttt
    82921 ctttgatgaa tatatttttt ctccaataaa atcaaaatca attaaattaa atgcagactt
    82981 atttatgtta gtcattatac tatttgctat ggatttattg gttcctgtaa tatcttcaaa
    83041 atatccttct tgtatccttt tttccttaac tttttgcact attaaatatt cacttaaact
    83101 atttgatata aatgttggag atacaacctt tgtacttatt tcatcacatt tttctattat
    83161 tagtggccag tatttaacta attctttctt cacaaaactt atatatgttg ttattttcag
    83221 ttcttcacta taatttaatc ttagagacct ttcatatcgt atctttgtgt tattgtttgg
    83281 tactaacaaa agaatacacc ttgaattatt accagtttta tctaataatt ctctatattt
    83341 atctaaagct tcatttccat attcttttaa actacttttt tcaaataaat attccatata
    83401 atcaccttcc tcaattaaat taagtgtata ttatctaatt atatcatatt gtgtatttta
    83461 caaaacacat tcaaattatt gcatttaact acaaaaaaag actgtatata tacttgcatt
    83521 cacacgtgca aatgtacata cagtcttact attgatacac ttatatttca ttttttgact
    83581 ttagtataaa tttacaacta taaatttaca ttaagttttt aaatattttt atttccattt
    83641 attttaatga atcttctatt cctttaccta tttgtgtacc catttctttt acttttgcag
    83701 atgagtttat taaactcata tcattttcat tatccaaaaa tgcacattct acaagtacag
    83761 ctggcatatc attacttctt aaaacgtata aataatcttt tgtagaatca ctaggaagta
    83821 ctcttgtttt tatacctcta tttgtaagtt taaatttctc taaaattcta tttaatatat
    83881 tggtagctac agttttagta gtccctccat ttttatcttt aagcttataa aatacttcta
    83941 caccatgacc ttgtttatta gttgagccat tataatgtat acttgtaaat aaatctggtt
    84001 tcaatgaatt agatagagca gttctatttc caagagataa agtcttatct gtgtctcttg
    84061 tcattattac attgaatccc tttgaacgta aatattcagt tgttgcaagt gaagtattta
    84121 aattgtaatc tttttcaatc attcctccag atgaatttcc aggtgctgaa ccaccatgcc
    84181 ctgggtcaat cataactgtc ttgcccccac tatttcctgg ttctgttgga gtcgtattgt
    84241 gtttagataa tgatgatgct atagagctca ttaccttaga agtcaatcct cctcctattt
    84301 tgtatacctt attagctgat ttagcttcta aattatcttt atggtatgca gatacatacg
    84361 tctttggtgt tataagtatt ggagacttta ggttcgcagc caatggacct gctgctaaag
    84421 catcaacaag tacatctgat ttagctacta aaacagcctc taactcatca tctgtataga
    84481 atttttttat tacgtttgca tttgtttcgt gtctatctgc tccgtatact ctattattag
    84541 taacactatc tttagttatt ccatttacct tatttataac atttgttgat atcatttgag
    84601 gaccacctat aaaataagca ttttgtaaat cctcactctt taaccattta tatgtattgt
    84661 ctgtaatact atctttatca gttaatataa ttggttgctt gtcttgacct gcttttgctg
    84721 ctatagtaag tgcatccact tctccgccat tagcatttgt tatgtatact ttttccacat
    84781 catgattttt atctatttcc tttgctatca ataaagatgt ctcatatcta ttagaaccat
    84841 ttaaacgtgt ttgactagca tttacagttg atttaatttg attagcagta gttttagaaa
    84901 tagcattctc atctccaatt ataattatat ctttagggtt taggcgcttt aattctgatt
    84961 ttacactatt aggtacattg tttttttcaa ccaaaagtat tggtgcatta tatgtggttg
    85021 ccagtggagt tgatataatg ccatctatac ttacatcccc atttattata actaccttat
    85081 ctgaaccatt tttccagccc tctttactta ttttaactgc tgtttcatat ctatcacttc
    85141 ctgtaagttc tgtaataggt gaagaagttt ttctcaaata tttatttaca tcttttctag
    85201 aagttatatc ttcaattata cattcctgtg ttacattata gtcttttaaa tttaatccca
    85261 gcttttctag gtcttgtctg tttaaagttt taggattatt tttgctgttt attgttatcc
    85321 cttgtagttc ccaaacattt agatattctg ggtcttggtt ataattgata gttccttcat
    85381 atgccccagt tgattgattg taatttagag ttgtattcaa tggcatgtca tagcatttat
    85441 actttaatgt aatgctatct gcatctggtt tttctttaaa tttgacacta acttttgatt
    85501 tgctacctaa aacgatactt tttttctcta agtttatatt taaatcagcg ttattttcca
    85561 tagcattaga ttcagtagca aatgccattg taggtgcgac gattgccatt gaaagaatcc
    85621 ctgttgttaa taattttgta tttcttctca tattaatctc cttacttaac ataaatttct
    85681 actttatttt taatctttta acttttgaaa atatgtcaaa gtgtatctaa cccaaaatat
    85741 aaataatata taagtaatat ataaaaataa acttataaaa ctcaaaaaat aaaaaaatta
    85801 aatatattta atacatttaa ttatctatac tatatcattt attgacataa aaatccacaa
    85861 aaaacacaca aacacacaat aaaatatagt aattaagctt ttattgtgta ttacaaatga
    85921 atactccaac tcttgacaat taactgtagc cttaagatat gtaaattctt catataatat
    85981 aaatcctttt acaattaatc ataaaaagac tgtatatacc tcatttcgca cttatatgca
    86041 caatgtatat acagtcttta ttactattat aattacattc ttatcttata gctttagttt
    86101 aaatttaaaa ttgcagatta atttatttta aatttttcta tgctctcatt gtagactatt
    86161 ttaatgaatc ttctatgcct ttacctattt gtgtacccat ttcttttact ttatttgatg
    86221 tattaagtaa actcatatct ttctcattat ccaaaaatgc acattctaca agtacagctg
    86281 gcatatcatt atttcttaaa acatataaat aatcttttgt agggtcagtt gatagtgttc
    86341 ttgtttttgc acctctattt tttagattaa atttctctaa gattctattt aatatatttg
    86401 tagctacagt tttagtagtc cctccatctt tatctttaag cttataaaat acttctacac
    86461 cattcccagt tgtatcagat gcattatagt gtatactcgt aaataaatct ggtctcaaag
    86521 agttagataa agcagttcta tttccaagag ataaagtctt atctgtgtct cttgtcatta
    86581 ttacattgaa tccctttgaa cgtaaatatt cagttgtcgc aagtgaagta tttaatgtat
    86641 agtctttttc tctaataccc cctaatggtt tacctgttgt tccagtgtcc gaaccaccat
    86701 gtcctgggtc aatcataact gtcttgcccc cactatttcc tggttctgtt ggagttgtat
    86761 tgtgtttaga taatgatgat gctatagagc tcattacctt agaagtcaat cctccaccta
    86821 ttttatatac cttatttgcc gatttagctt ctaagttttc tttatggtat gcagatacat
    86881 atgtctttgg tgttataagt attggagatt ttaagttcgc agccaatgga cctgctgcta
    86941 aagcatcaac aagtacatct gatttagcta ctaaaacagc ctctaactca tcatctgtat
    87001 agaatttttt tattacgttt gcatttgttt cgtgtctatc tgctccatat actctattat
    87061 tagtaacatt atctttagtt atatcattta ccttatttat aacatttgtt gatatcattt
    87121 gagggccacc tataaaataa gcattttgta aatcctcact ctttaaccat ttatatgtgt
    87181 tgtctgtaat actattttta tcagttaata taattggttg cttgtcttga cctgcttttg
    87241 ctgctatagt aagtgcatcc acttctccgc cattagcatt tgttatgtat actttttcca
    87301 catcatgatt tttatctatt tcctttgcta tcaataaaga tgtctcatat ctattagagc
    87361 cctttagacg tgtttgacta gcatttacag tcgatttaat ttgattagca gtagttttag
    87421 aaatagcatt ctcatctcca attataatta catctctagg atttaaacgc tttaattctg
    87481 attttacact attaggtaca ttgttttttt caaccaaaag tattggcgca ttatatgtag
    87541 ttgctagtgg agttgatata atgccatcta tacttacatc cccatttatt ataactacct
    87601 tatctgaacc atttttccag ccttctttac ttattttaac tgctgtttcg tatctatcac
    87661 ttcctgtaag ttctgtaata ggtgcagaag tttttcttaa atatttattt acatcttttc
    87721 tagaagttat atcttcaatt atacattcct gcgttacatt atagtctttt aaatttaatc
    87781 ccatcttttc tagttcttgt ttgtttaaag ttttaggatt atttttgctg tttattgtta
    87841 tcccttgtag ttcccaaaca tttagatatt ctgggtcttt attataattg atagttcctt
    87901 catatgactc agttgattga ttatagttta aagttgtatc caatggcata tcatagcatt
    87961 tatactttaa tgtaatgcta tctgcatctg gtttttcttt aaacttaaca ctgacttttg
    88021 aggtactacc taaaacgata ctttttttct ctaagtttat atttaaatca gcgttatttt
    88081 ccatagcatt agattcagta gcaaatgcca ttgtaggtgc gacgattgcc attgaaagaa
    88141 tccctgttgt taataatttt gtatttcttc tcatattaat ctccttattt aacataaatt
    88201 tctactttgt tttttaacct tttaattttt gaaattatat caaagtgttt ttacccaaaa
    88261 ttataaataa gtacataaaa aataaacttc taaaaaaatt ataaaaacaa aaaataaaaa
    88321 aattaaatat attgaatata tttaattatc ttcactatat catttattgc caaaaaaatc
    88381 tataaaagac aaataaaaac atataacaat tgtaggttta ttaaatttaa tacaacaaaa
    88441 aaagattacg tggctacg
//
LOCUS       DAYASV010000003        80173 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYASV010000003 DAYASV010000000
VERSION     DAYASV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 80173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80173
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.003"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(121..894)
                     /locus_tag="ACGI68_000198"
     CDS             complement(121..894)
                     /locus_tag="ACGI68_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HFH0136660.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1301)
                     /locus_tag="ACGI68_000199"
     CDS             complement(894..1301)
                     /locus_tag="ACGI68_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0136661.1"
                     /translation="MACETIKMEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVA
                     VSEACTNAIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLG
                     LFIIKTLMDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="ACGI68_000200"
     CDS             complement(1305..1622)
                     /locus_tag="ACGI68_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HFH0136662.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="ACGI68_000201"
     CDS             complement(1634..1894)
                     /locus_tag="ACGI68_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFH0136663.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="ACGI68_000202"
     CDS             complement(1894..2190)
                     /locus_tag="ACGI68_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFH0136664.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACGI68_000203"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="ACGI68_000203"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFH0136665.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACGI68_000204"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="ACGI68_000204"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFH0136666.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACGI68_000205"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="ACGI68_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFH0136667.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="ACGI68_000206"
     CDS             complement(7740..7946)
                     /gene="yaaA"
                     /locus_tag="ACGI68_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFH0136668.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8083..9189)
                     /gene="dnaN"
                     /locus_tag="ACGI68_000207"
     CDS             complement(8083..9189)
                     /gene="dnaN"
                     /locus_tag="ACGI68_000207"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFH0136669.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="ACGI68_000208"
     CDS             complement(9431..10750)
                     /gene="dnaA"
                     /locus_tag="ACGI68_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFH0136670.1"
                     /translation="MDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILLA
                     PSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYPN
                     LNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHHI
                     VSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERTQ
                     EEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIAI
                     LRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEAL
                     KDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTDL
                     SLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11409..11546
                     /gene="rpmH"
                     /locus_tag="ACGI68_000209"
     CDS             11409..11546
                     /gene="rpmH"
                     /locus_tag="ACGI68_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFH0136671.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11624..11968
                     /gene="rnpA"
                     /locus_tag="ACGI68_000210"
     CDS             11624..11968
                     /gene="rnpA"
                     /locus_tag="ACGI68_000210"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFH0136672.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            12000..12251
                     /gene="yidD"
                     /locus_tag="ACGI68_000211"
     CDS             12000..12251
                     /gene="yidD"
                     /locus_tag="ACGI68_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFH0136673.1"
                     /translation="MNKIIYILKEISKYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12283..12990
                     /locus_tag="ACGI68_000212"
     CDS             12283..12990
                     /locus_tag="ACGI68_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFH0136674.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12959..13588
                     /gene="jag"
                     /locus_tag="ACGI68_000213"
     CDS             12959..13588
                     /gene="jag"
                     /locus_tag="ACGI68_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFH0136675.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13700..15079
                     /gene="mnmE"
                     /locus_tag="ACGI68_000214"
     CDS             13700..15079
                     /gene="mnmE"
                     /locus_tag="ACGI68_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFH0136676.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15174..17069
                     /gene="mnmG"
                     /locus_tag="ACGI68_000215"
     CDS             15174..17069
                     /gene="mnmG"
                     /locus_tag="ACGI68_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFH0136677.1"
                     /translation="MIKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSI
                     ALMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQAD
                     KFKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVY
                     LNSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMS
                     LQEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPR
                     YCPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLEN
                     CKIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINA
                     VRKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLT
                     QKGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGIS
                     LYEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLE
                     NKKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRR
                     TRGGKGE"
     gene            17062..17781
                     /gene="rsmG"
                     /locus_tag="ACGI68_000216"
     CDS             17062..17781
                     /gene="rsmG"
                     /locus_tag="ACGI68_000216"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFH0136678.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKDEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17944..18729
                     /gene="noc"
                     /locus_tag="ACGI68_000217"
     CDS             17944..18729
                     /gene="noc"
                     /locus_tag="ACGI68_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HFH0136679.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18864..19637
                     /locus_tag="ACGI68_000218"
     CDS             18864..19637
                     /locus_tag="ACGI68_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0136680.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19639..20502
                     /locus_tag="ACGI68_000219"
     CDS             19639..20502
                     /locus_tag="ACGI68_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFH0136681.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20535..21677
                     /locus_tag="ACGI68_000220"
     CDS             20535..21677
                     /locus_tag="ACGI68_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0136682.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21811..22386)
                     /locus_tag="ACGI68_000221"
     CDS             complement(21811..22386)
                     /locus_tag="ACGI68_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HFH0136683.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKTQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22646..23938
                     /gene="rstA"
                     /locus_tag="ACGI68_000222"
     CDS             22646..23938
                     /gene="rstA"
                     /locus_tag="ACGI68_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin production/motility transcriptional
                     repressor RstA"
                     /protein_id="HFH0136684.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24043..24645
                     /gene="yedF"
                     /locus_tag="ACGI68_000223"
     CDS             24043..24645
                     /gene="yedF"
                     /locus_tag="ACGI68_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFH0136685.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24718..24981
                     /locus_tag="ACGI68_000224"
     CDS             24718..24981
                     /locus_tag="ACGI68_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFH0136686.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24996..25181
                     /locus_tag="ACGI68_000225"
     CDS             24996..25181
                     /locus_tag="ACGI68_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFH0136687.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25284..26480
                     /locus_tag="ACGI68_000226"
     CDS             25284..26480
                     /locus_tag="ACGI68_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0136688.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26694..26972
                     /gene="rpsF"
                     /locus_tag="ACGI68_000227"
     CDS             26694..26972
                     /gene="rpsF"
                     /locus_tag="ACGI68_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFH0136689.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27009..27443
                     /locus_tag="ACGI68_000228"
     CDS             27009..27443
                     /locus_tag="ACGI68_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0136690.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27462..27689
                     /gene="rpsR"
                     /locus_tag="ACGI68_000229"
     CDS             27462..27689
                     /gene="rpsR"
                     /locus_tag="ACGI68_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFH0136691.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27832..28152
                     /locus_tag="ACGI68_000230"
     CDS             27832..28152
                     /locus_tag="ACGI68_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFH0136692.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28160..29116
                     /locus_tag="ACGI68_000231"
     CDS             28160..29116
                     /locus_tag="ACGI68_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HFH0136693.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29127..31121
                     /locus_tag="ACGI68_000232"
     CDS             29127..31121
                     /locus_tag="ACGI68_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFH0136694.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31122..31571
                     /gene="rplI"
                     /locus_tag="ACGI68_000233"
     CDS             31122..31571
                     /gene="rplI"
                     /locus_tag="ACGI68_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFH0136695.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31590..32918
                     /gene="dnaB"
                     /locus_tag="ACGI68_000234"
     CDS             31590..32918
                     /gene="dnaB"
                     /locus_tag="ACGI68_000234"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0136696.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33123..33431
                     /locus_tag="ACGI68_000235"
     CDS             33123..33431
                     /locus_tag="ACGI68_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136697.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33513..34802
                     /locus_tag="ACGI68_000236"
     CDS             33513..34802
                     /locus_tag="ACGI68_000236"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFH0136698.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35128..35203
                     /locus_tag="ACGI68_000237"
     tRNA            35128..35203
                     /locus_tag="ACGI68_000237"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35161..35163,aa:Lys,seq:ttt)
     gene            35210..35284
                     /locus_tag="ACGI68_000238"
     tRNA            35210..35284
                     /locus_tag="ACGI68_000238"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35243..35245,aa:Glu,seq:ttc)
     gene            35289..35364
                     /locus_tag="ACGI68_000239"
     tRNA            35289..35364
                     /locus_tag="ACGI68_000239"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35322..35324,aa:Val,seq:tac)
     gene            35398..35472
                     /locus_tag="ACGI68_000240"
     tRNA            35398..35472
                     /locus_tag="ACGI68_000240"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35430..35432,aa:Thr,seq:tgt)
     gene            complement(35855..36847)
                     /locus_tag="ACGI68_000241"
     CDS             complement(35855..36847)
                     /locus_tag="ACGI68_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0136699.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36856..37914)
                     /locus_tag="ACGI68_000242"
     CDS             complement(36856..37914)
                     /locus_tag="ACGI68_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFH0136700.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38039..39502
                     /locus_tag="ACGI68_000243"
     CDS             38039..39502
                     /locus_tag="ACGI68_000243"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HFH0136701.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39604..40401
                     /locus_tag="ACGI68_000244"
     CDS             39604..40401
                     /locus_tag="ACGI68_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0136702.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40414..42516
                     /locus_tag="ACGI68_000245"
     CDS             40414..42516
                     /locus_tag="ACGI68_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0136703.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42584..43063
                     /gene="rlmH"
                     /locus_tag="ACGI68_000246"
     CDS             42584..43063
                     /gene="rlmH"
                     /locus_tag="ACGI68_000246"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFH0136704.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43212..43523
                     /locus_tag="ACGI68_000247"
     CDS             43212..43523
                     /locus_tag="ACGI68_000247"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0136705.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43571..43888
                     /locus_tag="ACGI68_000248"
     CDS             43571..43888
                     /locus_tag="ACGI68_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0136706.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44101..44802
                     /locus_tag="ACGI68_000249"
     CDS             44101..44802
                     /locus_tag="ACGI68_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0136707.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45026..46327
                     /locus_tag="ACGI68_000250"
     CDS             45026..46327
                     /locus_tag="ACGI68_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0136708.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46464..47558
                     /locus_tag="ACGI68_000251"
     CDS             46464..47558
                     /locus_tag="ACGI68_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFH0136709.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIILLDFIEPWSKFKLILEGE"
     gene            47560..48693
                     /locus_tag="ACGI68_000252"
     CDS             47560..48693
                     /locus_tag="ACGI68_000252"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HFH0136710.1"
                     /translation="MKIDILIKCGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELRRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48711..49820
                     /locus_tag="ACGI68_000253"
     CDS             48711..49820
                     /locus_tag="ACGI68_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HFH0136711.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49885..50625
                     /locus_tag="ACGI68_000254"
     CDS             49885..50625
                     /locus_tag="ACGI68_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HFH0136712.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGHVLIGILSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50630..51622
                     /locus_tag="ACGI68_000255"
     CDS             50630..51622
                     /locus_tag="ACGI68_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFH0136713.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYITTGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51810..52691)
                     /locus_tag="ACGI68_000256"
     CDS             complement(51810..52691)
                     /locus_tag="ACGI68_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0136714.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52964..54352
                     /locus_tag="ACGI68_000257"
     CDS             52964..54352
                     /locus_tag="ACGI68_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HFH0136715.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYANGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54380..55333
                     /locus_tag="ACGI68_000258"
     CDS             54380..55333
                     /locus_tag="ACGI68_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0136716.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIEPLTKEKQAGVSLG"
     gene            55393..55617
                     /locus_tag="ACGI68_000259"
     CDS             55393..55617
                     /locus_tag="ACGI68_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136717.1"
                     /translation="MNEKSSFKQIEIILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55610..56122
                     /locus_tag="ACGI68_000260"
     CDS             55610..56122
                     /locus_tag="ACGI68_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFH0136718.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56154..57008
                     /locus_tag="ACGI68_000261"
     CDS             56154..57008
                     /locus_tag="ACGI68_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HFH0136719.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57542..58336
                     /locus_tag="ACGI68_000262"
     CDS             57542..58336
                     /locus_tag="ACGI68_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0136720.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58440..59246
                     /locus_tag="ACGI68_000263"
     CDS             58440..59246
                     /locus_tag="ACGI68_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFH0136721.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SIIVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     VIHQLFNFLIGIINGDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59308..59829
                     /locus_tag="ACGI68_000264"
     CDS             59308..59829
                     /locus_tag="ACGI68_000264"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFH0136722.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60244..62331
                     /locus_tag="ACGI68_000265"
     CDS             60244..62331
                     /locus_tag="ACGI68_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0136723.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMDDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLVEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62346..62633
                     /locus_tag="ACGI68_000266"
     CDS             62346..62633
                     /locus_tag="ACGI68_000266"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0136724.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62660..63931
                     /locus_tag="ACGI68_000267"
     CDS             62660..63931
                     /locus_tag="ACGI68_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFH0136725.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63989..64588
                     /locus_tag="ACGI68_000268"
     CDS             63989..64588
                     /locus_tag="ACGI68_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0136726.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQEYLKWHPAGSLGKSIREGI"
     gene            64593..65498
                     /gene="dapA"
                     /locus_tag="ACGI68_000269"
     CDS             64593..65498
                     /gene="dapA"
                     /locus_tag="ACGI68_000269"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0136727.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPGKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKSQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65604..66236
                     /locus_tag="ACGI68_000270"
     CDS             65604..66236
                     /locus_tag="ACGI68_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HFH0136728.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGEILCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGLNIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNANKIREIIKNVRKI"
     gene            66713..69220
                     /locus_tag="ACGI68_000271"
     CDS             66713..69220
                     /locus_tag="ACGI68_000271"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0136729.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="ACGI68_000272"
     CDS             69562..71724
                     /locus_tag="ACGI68_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFH0136730.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVAISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRELNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="ACGI68_000273"
     CDS             71729..72346
                     /locus_tag="ACGI68_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0136731.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNEKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLDLSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="ACGI68_000274"
     CDS             72535..72879
                     /locus_tag="ACGI68_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFH0136732.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73449..74315
                     /locus_tag="ACGI68_000275"
     CDS             73449..74315
                     /locus_tag="ACGI68_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0136733.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPINK
                     ESGKPCINLQPDFKCSVHNSLIKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSPELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMGPEQIINLD
                     IISHRTKVNLLLSQASESMMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74428..74832
                     /locus_tag="ACGI68_000276"
     CDS             74428..74832
                     /locus_tag="ACGI68_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HFH0136734.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74996..75445
                     /locus_tag="ACGI68_000277"
     CDS             74996..75445
                     /locus_tag="ACGI68_000277"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0136735.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75601..75978)
                     /locus_tag="ACGI68_000278"
     CDS             complement(75601..75978)
                     /locus_tag="ACGI68_000278"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0136736.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76249..77157
                     /locus_tag="ACGI68_000279"
     CDS             76249..77157
                     /locus_tag="ACGI68_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HFH0136737.1"
                     /translation="MNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKCMNV
                     PIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWGTCKETIENSINAF
                     IKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINRLPV
                     LIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDIETL
                     NTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKYYLE
                     KTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            77641..79059
                     /locus_tag="ACGI68_000280"
     CDS             77641..79059
                     /locus_tag="ACGI68_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFH0136738.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            79135..79947
                     /locus_tag="ACGI68_000281"
     CDS             79135..79947
                     /locus_tag="ACGI68_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0136739.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
BASE COUNT        30662 a         9429 c        13376 g        26706 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ccttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa acacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttgacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctattgcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctagtaagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat atctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 tacaatactt ttattttagt tccttctatt tcaactatat ctcctgattt tatctttttt
     7801 cctcttctta attctatttc atcatttact gtcactaaac cttcttgaat taaaaatttt
     7861 gcatgacctc ctgttgatgc aatctctgct aatttaagaa attgatccaa ctttatatat
     7921 tctgattcta tagttatttc agtcataata gttcctcctt gtcactattt taaacaaatt
     7981 cctattgata cactaaaagt atattatacc ataaatatgc ttataaaagg agttgtctaa
     8041 gattagaaaa attttctaca aaagacaact ccttcatgtc tgctatatat ttgatgatat
     8101 tcttactgga agcagtaagt aaatataatt aacatcatct gttggtttaa ttatacaagg
     8161 atttacattt gtagtaaatt ctataaatat ttcctcatta tctatatttt tcaaaccttc
     8221 gataaaatat ctagaattga aagctatatc taggtaatct ccatctaaat ctatttcaac
     8281 ttcttcatac acatttcctt tttctgtatt agaagtaatt gccattactt tatctcttat
     8341 agataacttt ataagattat ttttttctga ttgagacaat aaagacgctc tctctatact
     8401 atttaaaagt tccttagtat ttaatttaac tctgctgtta tgttctcttg gcaataattt
     8461 tttataatca ataaaatctc cttcaagcaa tcttgtaata attttcgtat cattaattat
     8521 aaaaatagca tttttttcat taaatccaac tttaacatta tcctctccag aaagcaaact
     8581 atttacatct ataagtgtct ttccaggaat tatgactttt atattttcag ttaaactatc
     8641 aaccgaacaa ctttttacag ctaatctata tccatcaatt gctactaaat ttaagtttct
     8701 atctacaatt tctaaaagtt ctcccattaa aacaggtttt gtttggtcct gtgagatagc
     8761 aaatacagtt tgtttaatca tattttttag gatttcttga ggtatgctat ataaatcttc
     8821 ttcatttaat tctggtaatt taggaaattc ttttgctgca taacctttaa ttttaaatct
     8881 tgaatttaca caatttatgt aaatattatt ttctgaatca gtttctatct caacaaagga
     8941 atctggtaat tttctaatta tatctccaaa tagccttgca tctacaacaa catcaccttt
     9001 ttctatgatt tctgcttgta cataagtttc tattcctatt tcagcatcat atccagtaag
     9061 ttttaattga ccttcttctg tagatataag tatacctttt aataattcta ttgttgtttt
     9121 tccattaata gctttttgag caattccaat tctatttgct aggatttttt gattacaaat
     9181 tattttcaat gtggataacc tcctctggct ttttatatat aaaaataagt taaaatcata
     9241 gtagtaatag tagtaggtgt tgttgataat gttaataagt atgtataaca ttataaatac
     9301 aaagatatta acatgtgtat aagatgttaa tatctttgtt attttagact tgataatttg
     9361 ttaataattt ttgttcaaaa aaagtattaa caggttattc aagagatata aacatacaat
     9421 tgtgtataaa ttatcccttc aaatctgata taattttgtc tattttagtc ttaatttctt
     9481 cactttcttc tatatcctta gatactttgt catgagcatg aattactgtt gtatggtctc
     9541 tacccccaaa ctcttcacct attttaggaa gggataaatc agtaagctcg cgcgttaaat
     9601 acatagctat ctgtcttgga taagctattg aacgtgttct ttttttagaa ttaaaatctt
     9661 ccatctttaa attgaataca gaagatactt tttcttttat tctgagaaca tttatttctt
     9721 catttttaga agttgttata atatctttta aagcttctgt agcaagatca aaagatatta
     9781 ccctattgct aagagaagaa taagcaacca ctctagttaa tgcaccttct agttccctta
     9841 tgtttgactt aatatttttt gctatatatg acataacctc atttggcaca tctatacgtt
     9901 ctagttgagc cttttttcta agtatagcaa ttcttgtctc aaagtcaggt gcttgaatat
     9961 cagttataag tcccatttca aatctcgatc ttagcctatc ttctagtgta ggtatgtctt
    10021 ttggcggtct atcactagat ataattattt gtttgtttgc ttcatgaaga gtattgaatg
    10081 tatggaaaaa ctcttcttga gttctttctt ttcctgctat aaattgaata tcgtcgatta
    10141 gtaatacatc tacgtttcta tatttattcc taaattcttc gtttttatcg tcttttatag
    10201 agtttataag ttcattagta aatttttctg aagaaacgta aactactttt gagtcttttt
    10261 tttgactaac aatatgatga cctatagcat gcattaggtg agtcttacct aatccaactc
    10321 ctccatacaa aaaaagtggg ttataagctt tagctggaga ttctgcaact gcaacgcatg
    10381 cagcatgtgc aaatctatta ctattaccaa taacgaatgt atcaaatgtg tattttggat
    10441 ttagattggg gtaaagaact ctataattta aatctgtgct atcagaattt aaatcagcaa
    10501 cctctttttc gctcaataca aatttaatat tatatttttt taatgacagt tgactaatag
    10561 catcttcaat taaatgtaaa tatctattct ctaagatatc tttattaaaa tcactaggag
    10621 ccagtaagat taaatcatta agatgtattt ttaatggtac gatgttttta aaaaaggtat
    10681 taaaacttac tgaagttaag tcacctttta ttaattgtag ggttttgtcc cataaagaaa
    10741 ctatatccat aatataaacc tccgaacaga ataattaata ttttccacaa agttattaac
    10801 agaaaagcaa aaatatatat aaattttaca gaataactaa atttatggat aatggtgttg
    10861 ataaattaat aatagtataa attattaaaa aaatatgaaa agatataaac aatatgtaga
    10921 taaagttata aataacataa acatacaata aatggtgatt ataactagag tattgtcagt
    10981 aatcttaaaa gttatacaca aaagtatcca caagctgtga ataatttcta ataagttgat
    11041 aacatttatg cacattatca acaagtatat ttataatatc aaaatatatg tcaaacttca
    11101 atgatttgta actaatttat ccacaatatt aactaattgt tgataaaaat ataaacatgt
    11161 taaaattact ttatttgtgt atataattga aaaaaataat ttagaataaa attaaataga
    11221 tttttactag tattttcaat aaaaaaaata cataatatat atatggtaga ttttgtgata
    11281 taagcgtccc aaaaactcgt tgacaacagt agtaaaagta tttataatta tatgtgttat
    11341 gtttactttg agggtaaagt tattattttt ataaacggaa aataaaatct gaaaggcggt
    11401 gcaaaataat gagtaaaaga acttatcaac caaaaaagag acaaagaagt aaagaacatg
    11461 gtttcagaaa aagaatgaag acgtctaacg gaagaaacgt gttaaagaga agaagagcta
    11521 aaggaagaaa tagattaact cattaattac aaaagggccg ctgcaaaggt ggcctttttg
    11581 tgtaaataat tacaattcat agctgtgttt atggaggtta actatggact ttaataggac
    11641 taaagggttg aaaaaagact ctgattttag aaaagtatat aaacacggca aatcttttgc
    11701 aaacaaatat ttagtaatat atatacttaa aaataaatca gattatagta gagtaggtat
    11761 ttctgtttca aaaaaagtag gaaaagctat tactaggaat agagtaagaa gattaataaa
    11821 agaagcctac agattaaata ttgatgaaaa aattaagcct ggatatgata tagtatttat
    11881 agcaagggta tctagtaagg atgctacttt taaagacata gacaagtcta taaaaaattt
    11941 agttaaaaga acggatattt ctatttaatt gtatgctata aatatggagg ttaatgactt
    12001 tgaataaaat aatttatata ttaaaggaaa taagtaagta tttatcgaat ttatgtattt
    12061 atttagtgag attttatcaa aagtatatat ctcccttaaa gggacctacc tgtagatttt
    12121 atccaacttg ctcacaatat tcaatagagg cgtttaagaa atatggattt gtaaaaggta
    12181 tgtatttaac gataagaaga attttaaaat gtcatccttt tcatccagga ggttacgacc
    12241 ctctaaaata aaagagagta actattagga ggtataaaaa aattgaatat aataggaaat
    12301 ttcttaggat tgattcttaa ggtaatattt gaatttgtta atcattatgg tatttctata
    12361 atacttttta cgatacttgt aaagattatt ttattaccat taacaattaa gcagactaag
    12421 tcaacaaagg caatgcaaga tatacaacct cgtataaaag agatacaaga aaaatataaa
    12481 aataagccag aaaaacaaaa tgaagaaatt gttaagttat atggagaagc taaaattaat
    12541 ccactatctg gttgtttacc tttattaata cagtttccta tattaatagg attatttagc
    12601 gttttgagag aaccagttgc tcatggcgtt tttgcaaaca aagctgcatt tttagcagct
    12661 gacaatgggt ttttatggat aaaaagctta acatcaccag attatgtatt agcagtattt
    12721 tcaggagcaa gtgcatatgt catgcaaaaa gtaatgacac caaaagatca gttacaaggc
    12781 tctatgaaag taatgacata tgtaatggca ggtatgtctt tctattgggg atttatattc
    12841 ccagcaggac ttacattgta ctggacagta agtaacttat tctcaatagc tcaatattac
    12901 ttaataatga atcctttaaa agctaaactg gctgctaact ctaaggagga aaaagttaat
    12961 gagaaaaagc ctaaaattaa taaaaagtaa gagtaaagag gaagctataa gtaaagctat
    13021 agcagagttg aatttaaaag cagaggatat agaagtagaa atacttgaaa atccaagtaa
    13081 agggttttta ggttttatag gtgctaaaga tggtacttat gaaatttttg ttatcgagag
    13141 ggaagtagat gtagctaaaa attttataga ggtaatgttg aaaaatgcaa atgttgatgc
    13201 caaagtcaat gtatctcaaa aagataattt aatcatggtt gatattgaag gcaaagaagc
    13261 tgcaagttta attggtagaa gaggggaaac tttagattct attcaatttt tgactggatt
    13321 agctcttaat aaaataaata aagattctca tacaagagta cttgtagata tagaaaatta
    13381 tagatcaaaa cgagaagaat ctttaataag atatgctaat aaagttgcta gagaagtagc
    13441 gaaaacaaga aaaactaaaa aattagatta tatgaatcca tatgagagaa gaataataca
    13501 ttcagctctt caaaatgaca aatatgtaat tacatatagt gaaggaaccg atccatatag
    13561 aagattagta attgagtgta aaagataaag tcaacctcct aaacattagg gggttttatt
    13621 ttttaatatt attaaatcat taatgattgg ttaaatgtaa atctatatgt aaaaaattat
    13681 gaattaagag gtgataaatt tgtttataga tgatacaatt gcagcaatag ctacagcacc
    13741 tggagaaggt ggtataggta tacttagaat aagtggagaa aaagctttga aggttgcaga
    13801 agaaatcttt aaatctatgt ctggaaagag tattgaagaa tacaataaga gaacattaat
    13861 atatggaaat atagtggata atgaaaatat aattgatgaa gttttattgg catatatgaa
    13921 aggaccaaat tcctatacag gagaagatgt tatagaaatt aattgccatg gtggatttat
    13981 ttcggtaaag aagatattag aattaatact atcaaaagat gttagattag cagaggctgg
    14041 tgagtttact aaaagagcat tcttaaatgg aagaatagat ttatctcaag cagaggctgt
    14101 aattgatgtt ataaaagcca aaactgatat agctcatgaa gttgctcaaa atcaattaga
    14161 aggttcttta tcaaagaaga taagagaatt gagagataaa gtcactgaga tattagctca
    14221 tgtagaagtt gctatagatt atcctgagga agatattgag catataacat atcaaactct
    14281 taaagaaaaa actgatgaac ttaaaaaaga cattaaaaaa ttatatgata cagctgaaag
    14341 tggaaaaata cttagagaag gcttaaagac ggtaattgtt gggaaaccaa atgtaggtaa
    14401 atcttcacta cttaattcta ttttaggaga gaatagagca atagttactg atataccagg
    14461 aactactaga gatgttatag aagagtttgt aaacataaaa ggtatacctt taaaaattgt
    14521 tgacacagca ggtataagag atactgatga tattgttgaa aaaataggtg ttgaaaagtc
    14581 taaagaatca tttactagtg cagatttgat agtaatggtt ttagatgcat ctagaaagtt
    14641 gtcagaagaa gatatagaga tattagaaaa acttaaggat aaacaaacaa tagtgctgtt
    14701 aaataagaat gatttgaaac aagagattga agaagaaaaa atacttaaat atgtagaaaa
    14761 taatagtata ataaaaatat cagctttaca acaagaagga atagaagaat tgcaagataa
    14821 aatagagtct atggtatata aaggtagtat caaaaataat tctagtttag tagtaacaaa
    14881 ttcaagacat aaagatgcac ttagtaaagc atataaatct gcaacagatg ctttaattgc
    14941 tttggaacaa agtatgccat ttgactttgt agaagtcgat ttaaaaaata tatgggacta
    15001 tcttggatat attaatggtg atacagtaac agaggattta ctagataata tattccataa
    15061 tttctgtatt ggtaagtaat gtacaaaagt aaacttgttt ttattataga gatttttaaa
    15121 aaatttgaag tgtttctcat gaaacattaa ttttgaaaag aaaggaatta taaatgataa
    15181 agtttgaagc aggaaaatat gatgttatag tagtcggagc aggacatgct ggatgtgaag
    15241 ctgctttggc tactgcaaga atgggatata aaactttgat aataacaatg tctttggatt
    15301 ccatagcatt aatgccttgt aatccatcaa ttggagggac tggtaaaggt caattagtta
    15361 aagaaataga tgccttaggt ggtcagatgg gattaaatat agacaagaca tatatacaaa
    15421 gtagaatgtt aaatactgcc aaaggtccag cggtacactc tttaagagca caagcagata
    15481 aatttaaata tcatgaagaa atgaaaaaga cattagaaga tgaaccaaat cttgatatag
    15541 ctatggatga agttgtagaa atacttcatg aaggaaatgt tgtaatagga gttggaacta
    15601 aactaggatg ttcatttaaa tctaaagctg taatattggc cactggagtt tatctgaact
    15661 caaagatata tatgggtgaa gttgcatttt atgaaggtcc taatgcattg gggtatgcaa
    15721 aatatcttac agatagctta gttgagcttg gccttagaat gagaagattt aagacgggaa
    15781 ctccagcaag agttcataga gatagtatag acttctctgt aatgtcttta caagagggtg
    15841 atgaaaaagt aactccattc tcatttatga atgaaaatat agaaaaaaaa caagaacctt
    15901 gttatttaac aagaactaca gaagaaactc aaaaagttat actggataat ttaaaaaggt
    15961 ctgctatgta tagtggagta atagaaagta ctggtccaag atactgtcct tctatagaag
    16021 ataaagtagt aagatttagt gataaaacat cacatcaatt atttatagaa ccagaagggt
    16081 taaatactaa agaaatgtat atacaaggaa tttcaactag tttacctttt gaagttcaat
    16141 tagatatgta caaaactata aagggacttg aaaattgtaa gataatgaga cctgcatatg
    16201 ctattgaata tgattgtgtt gacccaactc aactaaaaat aagtttggaa ataaagggtg
    16261 ttgagaactt atttagtgca gggcaattta atggaacttc tggatacgag gaagcagcag
    16321 ctcaagggct tatggctggt ataaatgcag ttagaaagat agaaggaaaa gagccatttg
    16381 tattagaccg ttcagaagct tatataggtg tcttattaga tgaccttgtt actaaaggaa
    16441 ctaacgagcc ttacagaatg atgacatcaa gagcagaata tcgactatat ttaagacaag
    16501 ataatgcaga tatgagatta actcaaaaag gttatgatat aggattagtt aaaaaagata
    16561 gatatgaaag gttcctaaat aaaaaggctg ctgtagagaa agaatttgaa agactaaaaa
    16621 atgaaagagt tacaccaaaa gaagtaaata gtttgttaga agaaaaagga gcaacaccaa
    16681 taaaggttgg aatatctcta tatgaatttt taaagagacc agaggttact tacgaattgc
    16741 ttgaagaatt agggaaaggc gctggagaag atgtttcaag agaagttaaa gaacagtgtg
    16801 taataataac taaatatgaa ggttatatag agaaacaatt gaaacaaata gaccaattta
    16861 aaaaacttga gaataaaaag ctggatgaga aaataaatta ttcatctata gaaggtctta
    16921 gattagaggc tagacagaaa ctagatgata taaaacctat ttcaataggg caagcatctc
    16981 gtatatctgg tgtttctcca gctgatatat cagttttact tatataccta gaacaaatca
    17041 gaagaactag aggtggcaaa ggtgaataat atagaacttc tgaaaaacgg gattgaaggt
    17101 tttaatatag atacgaatga cagtatgtta gaaaaattta aaatctatag agagatatta
    17161 gttgactgga ataaaaagat gaatcttact ggaatagaag atgaaaaaga agtgtatata
    17221 aaacattttt tagactctat ttcagctgta aagaatggat atataaaaaa tgggatgtct
    17281 ataatagatg ttggtacagg tgctggtttt cctggaatac cattgaaaat atgtttagat
    17341 agcttagaac ttactttgtt agattcgtta aataaaagaa taaacttttt agaggaagtt
    17401 agtagagtac tagagttaga taatattaca tttatacatg gaagagctga agattttggt
    17461 aaagatgaag actataggga aaaatatgat gttgctacgg caagagctgt tgctggatta
    17521 ccgattctta tggaattttg tgtaccattt gtaaaagttg gagggtattt tatttgctta
    17581 aaaggtccga atgctaattt agagctagaa gaatcacaaa aggctattga tgttttagga
    17641 ttggagtata ttgaaaaaat agatgtagag ttaccagagg tagatttaaa tcataatata
    17701 ttagttttta aaaaagtgat ggaaacacca gttaagtacc ctagaaaagc tggtaaacca
    17761 gccaaaaatc ctattaagta atgaaaaatt tattattggt aagcttaatt atttctcagg
    17821 aaataattaa gcttactgta aaaaaacaaa aataaaggaa ttattaaagt agatgcagaa
    17881 ataaatttgt atgtacatat agagaacaaa aataatttat atctaaaata agagaggtat
    17941 gttatggaag ataaaagagt tatggagata cctatagaag atgtagttcc aaatccatat
    18001 caaccaagaa aaatattttc acaagtttca ttggaggaat tgagcaattc tataaaggta
    18061 tatggtataa ttcaacctat aacagttaga gcaaaagatg gcaaatatga actaattgca
    18121 ggagaaagaa gattaagagc agctaaatta gcagagctta aaaccatacc agcaattata
    18181 aataatatga acgatgaatc ttctgctgtt ctagcgttat tagaaaattt gcaacgagaa
    18241 gacttaaatt tcattgaaga agcaattggt tatgaaaact taataaaaga acatgctttt
    18301 actcaacagc aattagcaga aaaattgggc aaaaatcaat ctactgtagc aaataagctt
    18361 agaatattaa agcttccaaa cgatataaaa atgaagctaa tagagaataa tttaacagaa
    18421 agacatgcta gagcatttct taaactacca agtgaagatt taatgcaaag tgtgttagac
    18481 aaagtcataa aaaatgaatt gactgttaaa aaaacagaaa aattgataca agatgtatta
    18541 gaagaaacta aagttcaaga agagcctgat aaaaaacaga atataaaggg tgctatgagt
    18601 ataagaattt acataaatac tataaagcag gcctttgatg ctatatcaaa tacaggaatt
    18661 gatgctaagt ataatgaaat agataaggga gattatatgg aagttgttgt aaaaatccct
    18721 aaaaaataga gattactgct taccagtaat ctttttttgt aatataagcc ttgtaatata
    18781 aattgattta tagacattat ttatacttaa aaaacaccta ttcaacattt aaaattaatt
    18841 taattcttaa aggggggaac atcatgggca aagttatagc tgtattcaat caaaagggtg
    18901 gagtcgggaa aactacaaca aatgtaaatt taagtgctag cttaggaact ttgggaaaga
    18961 aaatattagt attagattta gacccacaag gaaatacaac tagtggatat ggtattaata
    19021 aaaacgaagt agaaaataca atctatgaaa ttatgttaga tgggttacat attaaagaag
    19081 caataataag tactgaattt gagaatgtag atgtagttcc atcagctact gaactatcag
    19141 gtgcagagat agagcttaca tctaaaacta atagagagta tattttaaaa aattccataa
    19201 aagcggtaat agatgaatat gattatatat ttttagattg tccaccctca ctggggatgc
    19261 ttacaataaa ttgtttaact gctgtagata gtgttttaat tccaatacaa tgtgaatatt
    19321 atgctttaga aggtgttagt caattaatgg agactataaa attagtaaaa tcaagattaa
    19381 atgctgatat agaaattcaa ggtgttgttt taagtatgtt tgatggaaga gctaatttat
    19441 caatacaagt agttgaagaa gttaagaaat actttaaagg tagtgtatat acaactttaa
    19501 ttccaagaaa tgtaagactt gcagaagctc caagtcatgg aaaacctgta atttattatg
    19561 ataaaagatg tagagggtct gtagcttatt tggagctagc agaagaattt atagatttag
    19621 aagaggagga atggtagtat ggagaataag tctaaaagaa gtaatagatt aggtagaggt
    19681 cttagtgcat tgataccaga aattaaagga gaaacttcag aaaaagaaat agtcaacata
    19741 gatatagaca aaatatatcc aaacgaagtt caacctagaa agcaatttga tgaagaaaag
    19801 ataaaagtat tgtcggattc tataaaaaat tatggtgttt tacagccaat agtggttaaa
    19861 atggatgaaa ataataaata tatgataata gctggagaaa gaagatttag agcatcaaaa
    19921 cttgcaaata aaaatcaaat acctgctata attaaagata tagatatgaa agatataatg
    19981 gagatagcct taattgaaaa cttacagagg gaagatttaa attcaataga agaggctcta
    20041 gcatataaga gtttaataga gcattacaat gtaactcagg aagaaatatc agaagctgtt
    20101 gggaagagta gacctcatat aactaataca ctaagacttt taaatcttgg acaagatgtt
    20161 atagaaatga tagacagtgg tagaatcaca gcaggccatg gtaaggcttt attgagaatt
    20221 gcagataaag atttgcaact tcaaatagcg aaaaaaatag aagaagaaga actttctgta
    20281 agagaagtcg aaaatatagc taaaaaaata tctgaaaata aacaagaaga acctaaaaaa
    20341 tctaaaccta aggatgtatt tattttagat gttgaagata aacttagaaa tatatttgga
    20401 acaaaggtga atatttctaa aggaaagaaa aaaggtaaaa tagagataga atactataat
    20461 gatgatgatt taaatagtat tgtatcaatg cttcttgaat agaacaaaaa tataaaacag
    20521 agaggaagat tgttatgatt tatttagata atgctgcaac tacatatcca aaaccagaaa
    20581 gagtgtataa tgctgtttta gattgtatga aaaattattg tgcaaatcca ggtagagcag
    20641 gccataaact tgctatgaga gctgctaggg agatatatga tacaagagag aatatagcta
    20701 agttgttcaa tgtaagtaat cctatgaata tagtatttac atctaatgca acagattctt
    20761 taaatttagc gataaaaggt gtattacaag aaggagacca tgtaataaca acgtctatgg
    20821 agcataattc agtaataaga cctataaaag cattggaaaa aaggggtatt gaaaatactg
    20881 tagtaaaatg cgattatgag ggatttttag attatgaaga tttagaaaaa tcaataaaga
    20941 gtaatactaa actgattgta acaacacatg cgtctaatgt atgtggaact ttaatagata
    21001 taaaaaaagt tggagaaata gctaaaaaac ataatatact atttttagta gatgcatcac
    21061 aaacagcagg tgtttatgat atagacgtaa atgaatgtaa tatagatatg ttggcaatgc
    21121 caggtcataa atgcttattt ggacctcaag gaacaggtat attatatgta agagaagggt
    21181 tgaacttaaa tatattaaaa gaaggtggaa caggtagtaa atcagaagaa atagttcaac
    21241 cagagttatt cccagataaa tatgaatcag gaactcataa tactccaggt atagcaggtc
    21301 ttaaccaagg tatactattt atatttgaaa gaggaatcaa caatataaga caacatgaag
    21361 aagaattatg tcaatatatg atagacaaat tagaggaagt accagatata aagatatatg
    21421 gaccaaagga cagtaaaaaa agagcatcag taatagctct aaatatagga gatatggatt
    21481 ctggagaggt tactttctta ttagatagtg attataatat agcgactaga tcaggaatac
    21541 attgttcacc actagcacat accactttag gtactttaaa acaaggtgca gtaaggttta
    21601 gtataggata tttcaacaca aaagatgaga tagataaagc agtggaagct ttgaaaaaaa
    21661 tttcaaaaaa taaatagcat gagatttaat aatatagaaa taaaaaaagg ggatatttca
    21721 aaataaaaat ttgaatcatc tccttttttg tacaaaaaag aaaaggggtt cctaagaacc
    21781 tctaatctat ttgataatag tttcaagaaa ttactttaag taatctcctt cttcaagaac
    21841 tatatgacca ctttcgtaat tagaaccatc aacagtaatt tttatttgtt caacattcat
    21901 attttttatg taagtacgag caactgaatc aagcattaaa gtttctgcat cactacctaa
    21961 gtttgacttt gtaaagtcag agctaacatc taatatacca gtttttacac catcaatgtc
    22021 ttttgtagta aatgaattta attttgcaga ttctggaaca actttaagtt tttgaagttc
    22081 ttcaaataaa gtgttctcat caattttatt taaatcaact ttattttcaa ttaaatctgt
    22141 cttatctaca tcaaaagagt aaatagtagc tgattgagtt ttagtttctt cttttggagt
    22201 aggtttagct ttgttattag gaacctcttt ttcagtattg ttttcttctt ttggttgctc
    22261 aacatttgta ttgctcttag tttctttttt agatgaatca cttccatttt gagcattagt
    22321 acaaccaact gcgaatatag atatagataa tatacttaac actaacatta ttttcttatt
    22381 tttcataaaa atttctcctc tcttaaaaac tattcttata tataattata gtcgttatga
    22441 gcaaatatat gaatacaaaa tagtaacaaa tttaaaaaat gatttattta aattaataaa
    22501 actcaaaata aactaaattt tagtcatttt tatgggaaat catttaatgt acagtgaaaa
    22561 tattgtaaaa taaaagtagt gtttttaaaa taaatataag ttataattat attataaaaa
    22621 aatgtcttca aaaggtggga atagtatgga aattttgagc ttaggagaaa agattaaaaa
    22681 attaagaaaa gaaaaaaaca tgacattaaa agaattagct ggagatagga taacagcagc
    22741 acaaataagc catatagaga gggataaatc tcatactagt tatgaactgt tggaatatct
    22801 aggcgataag ctagatgtaa gtgtagaata cttattagag accaaggaaa tgcaatcaaa
    22861 aaagataact gataatttaa tactacagag cgaaatttat ataaaagaaa atgaattaaa
    22921 caaggctgag gaccaaataa aagaagtttt agatatatgt aagagatacg atttaatgga
    22981 gaactatggc agatgtaatt ttttaatggc taatattaat ttgaaaaaat ctaattataa
    23041 tgatgctata gaaaactttg aaaaggcatt gtacttcttt ataaaaaata gtgatagtga
    23101 aaatatactt aagtgttatt taaacatagg gaaaatatat atgcaggaag acttttataa
    23161 aggtgctata agccacttta attttgcaga ggaaattttg tcagagaata aatttgagga
    23221 tgttaacata cacaaggaac tatatagtaa gatggcatat tgttacataa aactagataa
    23281 tccaaatatg tcattgaagt acatagaaaa gataaatgaa atagataata gaaatgatag
    23341 aaaagaagat gtcgatattt tggtgttaaa agcaaataat atgcttgaga ttggaaaata
    23401 tgaagaatca agagattact ttaaaaaagc actaaaaata cttgagagtg aggataataa
    23461 aactggatta gcaaatgtgt atttaacaat ttgtgatgtt tacagaaaaa taggtgaaac
    23521 agatagagta cttgaatatt cacaaaaggt atatgacata aagaaaaatg atgaagatga
    23581 atacatgatg tcaggattat ttaagataat agaagcatat atagataaag aagattatga
    23641 tttagctaga aaatattgta agatagcctt agcttcatca ataaaaaata aaaataaatt
    23701 caatgaatat aaagcattga gattctatgc taatatgtat aaaaatcaga atgaagtaac
    23761 tcttgcaatt gaatatctga ttaaatgcat aaaaatagta tcagatttag gcaacaatag
    23821 aatattggcc aatttatata tagacttagg tcaattatat tcaaatatat caaaagaaaa
    23881 agagttagaa tattatcaaa aaggtgtatt tatgtacaaa aatttagaaa taatgtaggg
    23941 taaataaacc tataagggta tagaaacaat ctataacaag caagataata tctatgataa
    24001 aatataaatt gtatgaatat ttttaaggag atgaatcaga caatgagtat aaaaatagat
    24061 gcgagagggc ttgcttgtcc taaaccagtt ataaatacaa aaagagaatt ggataaccta
    24121 gaagaaggtg ttgtagttac aatagtcgat aatgaaacag ctaaggagaa tatattaaaa
    24181 cttgctaaat ctttaagttg tgaagcaaat attgttgacg aaaaagagaa ttttatatct
    24241 atagaaatta gaaaaggtaa aaatatacta gataaacaaa atgttgtaga taataaagaa
    24301 agtgaattag acaatacatg tatatttata tcttcagata agatgggact tggaaatgac
    24361 gaattaggaa aagtgttaat aaaaggattt atatatacat taacagaatc taaaccttat
    24421 cctaagtatg tacttttagt taatggagga gtaaaattaa gtgctgaaaa tgaagaaacc
    24481 atagaaaatc taaaaatact tgagaatatg ggtgtagaag ttttatcttg tggtacttgt
    24541 ttagattatt ataatttaaa agacaaatta caagtaggta cagttacaaa tatgtatact
    24601 atagttgaga cattaaagaa tgcatcaaat accatttcta tataatgtat gtttgtttaa
    24661 taatataaaa ttaataataa agattaactc aaatagatag tcagaaagga ttaagtaatg
    24721 aatcaaatgt atatagtgtc atttaattcg acacatcatg cgataagatc agaaaagtta
    24781 tttggtgaaa atagtctaaa agtaatggct cttccaacac caagagaaat aacagcaagt
    24841 tgtggaatat caattaaatt ttcttttgaa gatatggaaa agataaaaac tattttagtt
    24901 gaaaataatg tagatataaa aggaatttat tgtataagta aattagaaaa tggctcaaaa
    24961 gaagtagaga agttagatta ggaggctaat tatttatgcc aatggattta aaaataggtg
    25021 atattgttga attaaaaaag caacatgcat gtgggtgtaa agagtttgaa atcataagaa
    25081 cgggaatgga tataaagata aaatgtacta aatgttcaag gctaataatg cttgatagag
    25141 agaccttaga aaagagagta aaaaagctaa taaaaaagtg ataaaaattt aacacttaaa
    25201 aaatgtcaaa taccgtatat taatatccaa aattttggta taataagaat tgaagaaaac
    25261 attttcatgg gggtgcttta attatggcag ttaaatatgc aaaaagaatg caaggattac
    25321 aaggatcaga aatacgtgag cttttaaaac ttactcaaca accacaaata atatcttttg
    25381 ctggtggaat gccggctcca gagttatttc cagttgagga aatgaaaaaa gtatcagttg
    25441 cagtgcttga agaaaatgga agatcagcta tgcaatatac tacaacagaa ggatatgaac
    25501 cattaagaga aaaaatagca gctagaatga atgataaaaa caaaactaat gtaaacaaag
    25561 atgatatatt agttacaagt ggttctcaac aaggtctaga ttttgcagga aaagtattta
    25621 ttgatgaggg tgatgtaatt ttatgtgaaa gtccatctta cataggagct ataaacgcat
    25681 ttaaatctta tcaacctaaa ttcatagatg ttccaacaga ttctgatgga atgataatgg
    25741 aagaattaga aaagatacta gaaacaactg atagaataaa aatgatttat gtaatacctg
    25801 acttccaaaa tccaacagga agaacttggc cacttgaaag acgtaaaaaa ttcatggaaa
    25861 tagtaaataa atttgaaata ccagtaatag aagataatcc atatggagat ttaagatttg
    25921 aaggagagac tttaccatct ttaaaatcaa tggatacaaa aggattagta atattcttag
    25981 gaactttctc taaaatattc tgcccaggat acagattagg atggacttgt gcttctccag
    26041 aaattttatc taagtttaac tttgcaaaac aaggtgcaga cttacaagca tcaactatat
    26101 ctcaaatgga agtaagcaaa tttatggata tgtatgacct agatgcacat gttgacaaaa
    26161 taaaagcagt ttatgttaaa cgtagagatg taatgcttaa gactatggaa gaagagttcc
    26221 cagaaggatt agtatttact catccagaag gtggattatt tacatgggta gagcttccaa
    26281 gtaatttaaa tgctaaagaa ttaatgccaa aatgtttaga taagaatgtt gcttatgttg
    26341 ctggaggagg attcttccct aatggtggta gagaaaatac tttcagactt aactattcaa
    26401 atatgccaga agaaaaaata atagaaggta taaaaaatat agcagctgta ttaaaagaag
    26461 ctatgggtgt agaagcttaa ttaaaaataa taacttatat ataatacaag ggatggttgt
    26521 tgccatccct tgtttgtttg aaaagtatat aattcacctt atactaaact ttgtccaaaa
    26581 tttaaattga cagaaataag aataaatgat aaaatgtgta ggtatgagat tttagtctca
    26641 tttctctgct ctaagagtgt agagccgtta agtccaaagg gaggtgaatt atagtgagaa
    26701 attatgaatt agtttatgtg gtaaagccaa actctgatga agaagtaaga gaggctatac
    26761 taaacaaagt taaggaagtt gttgcaactg acggagaaat agttaaagtt gatacttggg
    26821 gaactaaaaa attagcttat ccaatagcta agtttacaga aggtttctat gtattagtta
    26881 acttcaaatc agcagtagac gttcctaaag agatagacag aaacttaaag ataaatgaaa
    26941 acgtaataag acacatgata gttgttgctt aataactggt tttattataa aaaataggtg
    27001 gtgtttttat gaatcaagtt gtattagtgg gaaggttgac tagagaccct gaattaagat
    27061 acatcccagg tacaggaaca ccagtggcat cctttacaat agccattgat agagactatg
    27121 taaaaaaaga tggatctaaa gaaactgatt ttatacctgt tgaagtaatg ggtaaatctg
    27181 ctgaattttg tgctaactac ataactaaag gtagattagt agcacttcaa ggatctataa
    27241 gagtagacaa ttatcagaca caatcaggtg aaaagagaac ttttacaaaa gtaagtacta
    27301 gatcagtaca agctttggat agtaagaata aatcggaaaa ttcatataga gagagtgctc
    27361 cagcttttga gccaagcttt gaacctcaag gtttagaccc acaaggtttc caagctatag
    27421 atgatgacga tataccattt taaaaaggag ggaaaaaagt catgatgaat aaaaaaagac
    27481 gtaagaaaaa aagagtttgt caattttgtg ctgacaaaaa cgctaaaata gattacaaaa
    27541 gtactcaaag attacaaaag tatataactg aaagaggaaa gatattacca agaagaatat
    27601 ctggaacttg tgctaagcat caaagagaat taacagttgc tataaaaaga gcgagaaata
    27661 tagcactttt accatataca ttagactaaa aaaatgagcc ttgtgctcat tttttattat
    27721 atgtatttat atatctaaat gaggcgagct ataaagctcg tttttttgtt tatatactta
    27781 aatatattat tatttatgat ataatattat atattaagga gggttgtaaa cgtgaaaata
    27841 gataaaggtt ctgagataac gagaaacata aagataatag aatggatgaa aacagaaata
    27901 ttaatgagtg taagtgattt gtttaattta ttgtttaaag gggtaaaacc attagatgaa
    27961 gcaattcaag atactttagc aaatataata atgataacat atctcttagc aaagagatta
    28021 ggaattagtt ttagagatgt ggattataaa gtcaaggaaa aaataaaaat tggtatagat
    28081 gaggaccata gcgttgaaag atggtatgga gatttatcta atttaaaaaa acatatggat
    28141 aatagggagt gatattaaat tgaataataa aattagacta tctaaggcaa tgtctataat
    28201 agttttagct ataataatcg ctttagtaat agcctatatt ccaatgctag gaatatttag
    28261 tatgttagca gctgttccat atgtaattat aggtgctatt actgatagaa agtattctct
    28321 tatatctatt ttggttacat tttgtgtact gattctattt gcagacattt cttatgcatt
    28381 aaatatatgt ataatgtatt ctttaccagg tatagtaata ggaaagatgc taaagagaag
    28441 ctttgaacaa gaggacagta ataaattcga accgatatat ggtggaacca taatatttgt
    28501 actttcaatg cttgtatatt tttttgtatt aaagacattt ttaaatgtga atctattaga
    28561 tgaagtttct aagatgatat cagaaattgt aaatattcaa aaaaatagtt tttctgcaac
    28621 tgaacttaaa gtgtttgata aaatgaagcc agatgagatt gtaagttatt ttacaaatat
    28681 gctaccaatg atgttatttt tacaagggtt gttatcagcg tttgtcacat attatttgag
    28741 tgtatttttt ataaaaagga ttacaaagat gaatataaga tttcctaagt ttgctgattt
    28801 ttatttaccg ggaaatgcta tactcacaac tttcttactt tatttattag tcttatttat
    28861 tcaaattatt ggttcaaaat tatatacaga attaataatg actaatttac agctagtttt
    28921 taatttaatg tttgtaattc aaggtatagc agtttgcatt tatttcttaa agaaatggat
    28981 aagacaggga ccaaataaaa ttatgttttt ttcagggata attttatgtt tatttggatt
    29041 tatgggaata tcttttgttg gtatggtaga tagtataatt gactttagaa aggtaagaag
    29101 ctataaatcc acttaggagg atagaaatga gtaataaaca aacctttaaa ttaaatatgc
    29161 cagaaataaa tttatatata attgttattg gtatttctag tataattttg ctttattaca
    29221 acttgtatgt agggtgttta tttttctgta tttttgtata tatggttttt cacaattgga
    29281 gaactactaa tattagacgt catgaatgga cagagtatat tcaaaattta tctttagata
    29341 tagatgaaac aactaaaaaa gccataataa atcttccaat accattatgt attttagaat
    29401 ttgatggaaa tatatcctgg tataatggaa agttttatga tatgattggt caaaaagatt
    29461 tacttgataa aaatatagag gatatagtta aaaatcttaa tttaagaaaa gtattaaatg
    29521 aaaataaaga gatgtacaca gaaataaact ataaagaaaa agaatataca ataatttata
    29581 atgttataaa aaatgatcaa gaaaaaaatc ctaaatattt gatgatatta tactggatag
    29641 acaagactga atatttaaaa gtaaaacaaa attatgatga tgaaaaaaat gcaatgatgc
    29701 taatacaagt agatggatat gatgaagtat taaaaagtgc agcagaagat aagagagctt
    29761 taattaatgt tgaagtagag aaaatacttt ctgctttaga attaaattca aatggtgcat
    29821 tgagaagaac ttcaaaagac aaattcttct tggtaatgca taaaaaagag ttaaaaaaat
    29881 tggaagctga gaagttttct attttagata caataagaca tattgattat ggaaataatc
    29941 ttcctgttac tataagtata ggaattggta tagatggaga tacattaaat gagaacctaa
    30001 aacttgctac gggtgctctc gatttagcac ttggtagagg tggtgaccag gctgttgtaa
    30061 aaacaaaaga taaatttgtg ttttatggag gaaaatctaa agctgtagaa aagaaaacca
    30121 aagtaaaatc cagattaata ggtcatgctt taagagaggt tatacaacaa agtgaccaag
    30181 tttatataat gggacataaa tatcctgata tggatgctat gggagcagca gttggtgttt
    30241 atgatatatg taaatcctgt aacaaaactg caaacatagt tttacaatct gtaaatgaat
    30301 ctatagaaat atttataaat aaaataaatg aaaataatta ctataaaaag ttgtttattg
    30361 gaaaagagga agctattgac aactgcacta aaaatacatt agttgtagta gttgatactc
    30421 atagaccaaa ttatactgaa tgtgaagaac tgttaaagct atcagagaag gtagtagtta
    30481 tagaccatca tagaagaggt gtagagttta taaatgatgc agtgctttta ttccatgaga
    30541 tatatgtatc ctcaacgtgt gaaatggtta cagagttagt tcagtatatg gatgaagatg
    30601 taacaataaa taaacttaca gctgaagggc ttttagcagg tataagtcta gatactaaaa
    30661 actttgcatt caaaacaggt gttagaactt ttgaagctgc ttcttacctt agaaaagttg
    30721 gagcagatac tatagaggta aagaaattct tcaattctga tgtaaaagat ttcataataa
    30781 aagctgaaat aattcaaagt actaaaataa ttaataatag aatatgttta gcatattcaa
    30841 gtactgagat agatagtata aatgttataa tagctcaaac tgctgatgag ttattaaata
    30901 tcaaagaagt agaagcttcc tttgtattag gtgaaaaaga tgatactata tttataagtg
    30961 cgaggtcttt aggacaaata aatgtacatg tacttatgga aaaattagga ggcgggggac
    31021 acatagatat agctggagca cagttaaaaa atgtttctct aaaagaagct tataagatgg
    31081 taaataaaat aatagaggag tatttggagg aggaagaata gatgaaagtt atattattaa
    31141 aagacgtaaa aggaactggt aaaaagggag aaatgaaaga agtaagtgat ggatatgcaa
    31201 gaaatttctt atttcctaaa aagatggctg tgcaagcaga tagtgtagca ataaaagaat
    31261 taaatgaaaa gaataaatct aaagaaataa aagcacaaaa agagtatgaa gaagcagttt
    31321 tattaggtaa gcaaatggaa gaaattaata tagaaatata ttcaaaagca ggagaaggtg
    31381 gaagattatt tggttcaata acttctaaag aaattgctga gcaattaaag aaacaaaaag
    31441 acatagatgt agataaaaga aaaatattat tagatgaacc aataagatct ttaggttcaa
    31501 ctttcgtaga aataaaaata catcaaaaag taactactaa aataagagtg gatgtaaaag
    31561 aaaaacagta ataactagag gtgattaaga tggaagatat gacgagaatt cctcctcata
    31621 gtgtagaatc agaacaatca atattgggtt ctatacttct agataaagat gctataataa
    31681 cggttacaga aactataaag cctgatgatt tctataaaga ggctcacaaa ataatatatg
    31741 aatgtatgat aactttaagt aataaaggtg agcctataga cttgataaca ttaacagaag
    31801 aactaagaaa acaagggcat cttaatgata ttggaggaat cagttatatt acaagtcttt
    31861 ctactatagt accgacgact tcaaatgtaa aatactatgc tgatatagta aaagaaaagt
    31921 ctgtacttag aaagcttata aaagcttcta atgaaataat aaatctagga tatagtggag
    31981 caactaagat tgaagatgtt ttagaacaag ctgaaaagag tatatttgat atatcacagg
    32041 agaaaactag tgatgatttt aaatctataa acttagtttt gatggatgcc tatgatatga
    32101 tagaaaaatt atacactaac aagagtgatg tcactggtat aactactggt tttaaagatt
    32161 taaacaaaaa aataaatggt cttcaaagga ctgatttaat attaattgca gctagaccag
    32221 caatgggtaa aactgccttt tctttaaact tggttcaaaa tgcagcctta aaaggtgatg
    32281 cctctgtagc tgtatttagt ttagagatgt ctaaggaaca attagtacaa cgtatgttat
    32341 cttcacaatc tagtgtagaa ttaaaaaaaa taaagactgg aacacttaat gataatgatt
    32401 ggcctagaat tatagatgct atggcagtat tatcagatgc caaaatacac atagatgata
    32461 ctcctggtat aaaaatatca gaactaaggt caaagtgtag aaagttaaaa atagaaaaag
    32521 gactagattt agtgcttatt gattatcttc aacttatgga aggtgaaggt aataatgaaa
    32581 gcagacaaca agagatatct aagatatcaa ggtcactaaa aatattagca aaagaattaa
    32641 actgtcctgt agtagcacta tctcagttat cacgtgcacc agaacaaaga gcagaccata
    32701 gacctatgtt atctgattta agagagtctg gagcaataga acaggatgca gatatagtta
    32761 tgttcttata cagggacgaa tattatcatg ctgattctga gagtaaaaat ataggagaag
    32821 taataatagc aaaaaataga cacggtgaaa ctggttctgt ggaacttgtt tggcttggtg
    32881 aagttcaaag atttggggat aagttgagag acctataatc taacgaatat ccacagaacg
    32941 tcctttcttt ctacaggaaa aatgtggata catgtcacta aatatgtcca aacattgaaa
    33001 tttagctaaa aaactatcca cactatccac agatggcctg ttgataagtg tgaataagta
    33061 gataaagcaa gcttttggct tgctttatct gtgtataaat attaattatt aggaggaata
    33121 atatggaaaa aataagctta aaatatatat atcccaatat aattaaagta cttgatgaaa
    33181 taaacttatt tagagtaatt gacaataatt taagagaatc tattgttgtt tatgctaata
    33241 atgtggataa tcagtatcat ataaacatga ctaatacaaa ctttggaaat ataattaaca
    33301 tatgtaaatt agaaaaactt ttagatgtgg ataaatttat ggaaaaagtt ataaaatatg
    33361 aaaaagaaat aatagagaaa gaagagtttt caaaaataga agaatatatg ttaaatattg
    33421 gagaatacta aaaaagttga aaaaaatacg aatgtttgat agaataagta agtaaagtgt
    33481 tcgagagtat ataaaatata gaggtgaatt acatgaaaac agttgcaatt gttggttctc
    33541 aatggggaga tgaaggtaaa ggtaaagtta tagattatct tgctactcaa gcagatgtag
    33601 tagtaagagg gcaaggtgga aataatgcag gacatacatt agttgtagaa ggtaaaaaat
    33661 acgcattaca tctaattcca tctggagttt taaatccaaa tacagtgaat ataatcggaa
    33721 atggaatagt gtttgaccct aaaggatttt tagaagaatt ggaaatgttt aaaacggata
    33781 atataagtac agaaaatata aaaataagtg atagagcaca tgtcatattc ccatatcata
    33841 aagaactaga tgcattgtca gaagaagcca gaggcgattt aaagataggt acaacaaaaa
    33901 aaggtattgg accttgttat atggataaaa cagagagatc tggtattaga atatgtgact
    33961 taatggataa agataaattt gctataaaat taaaagctca aatagatgct aaaaatgaaa
    34021 tagttaaaaa tatatatgga aaagaagaat tatttgattt tgaaactata tataacgaat
    34081 atctaggata tgcagagcaa ataagaaaat atgtggcaga tacgtctgtt attgtatatg
    34141 atgcagtaag agcaggtaaa aaagtattgt ttgaaggagc acaagggact ttattagatt
    34201 tagatttagg aacttatcca tttgttacat cttctcatcc aacatctggt ggatttgcaa
    34261 ttggtgcagg tatagggcca aatatgataa aagacgttgt tggtatagta aaagcatata
    34321 ctacaagagt tggagaagga ccgtttgtta cagagcaaat aaatgaaact ggagataaga
    34381 taagagaaca aggacatgaa tttggggtaa ctactggtag accaagaaga tgtgggtggt
    34441 ttgatgcagt tatagttaag tatgcagcta gagttaatgg attgactagt atttcattta
    34501 tgttacttga tgtattaact ggatttgata agataaaagt ttgtacgtct tataaaatgg
    34561 gcgataagat aataacggat ttcccagctt cattagatga tttagcaaaa tgtgaacctg
    34621 tatatgaaga attagatgga tggaatgaag atataactca aatagataac tttgatgacc
    34681 ttccagaaaa tgcaaagaaa tatgtagcaa aaatagaaga actagttgga gtaagtgtag
    34741 atatggtttc tgttggtcca aatagagctc aaactataat aagaagaaat atatttgctt
    34801 aatattttat caattaaata atttaaaata gttgaagaag agtatctcaa gataatgttt
    34861 atagttatta gagatgctct tttatatttg acttacattg attttttatt aagttaaagc
    34921 tacttataca tattctaact gaaattaaag ttgaatttat gtagatacat ataggttttt
    34981 taaaaagaat tttgttgaaa ataaatggct aaagatgatt ttaattatat tctaaaaaaa
    35041 gtaaaaaaaa ctattgacta aaaattatat agacgctata atataacttg tgagtgaaag
    35101 aaacgacgaa aaaagtcgaa aaaatgtggt ctattagctc agtcggtaga gcacctgact
    35161 tttaatcagg gtgtcccgcg ttcgagtcgc ggatagatca ccaaaatatg gctcgttggt
    35221 caagaggtta agacaccgcc ctttcacggc ggtaacaggg gttcaattcc cctacgagtc
    35281 accagtatgg gactatagct cagctgggag agcacctgcc ttacaagcag ggggtcacag
    35341 gttcgagccc tgttagtccc accaaatttt gaattagaga taaatactag attatatgct
    35401 ggtgtggctc aacggtagag cagctgactt gtaatcagca ggttgtaggt tcgattccta
    35461 tcaccagctc cacaaagtac ataaattcaa attaatataa ttttctatgt tacaggatac
    35521 ttttaagtat ctttttttaa tttcattttt attgtattaa aaatgaaatt aaacatacaa
    35581 caaaattttg aattacttgc aatgtgatag ttttaaatat aaaatttaga atcattctag
    35641 cataatatta tgtaatatag caaatttaat ttatagtatc aataaatata gactactaag
    35701 aatgtaaatg atttgttaga ttaaggtaca ttagatatgt aaatagaaaa ataaaaagta
    35761 tgttttaatt tatgtgatat aagttttaaa cagagaagca agtttgctct tacctctcta
    35821 tttaaaaata aaaaaatgtt ttttaagggc tattttattt aaatctttcc catctcaaat
    35881 catctccagt aaattctaaa atcctaaact tatctaatat tctagagaga gttctttgag
    35941 tgtaagtatt tcctatttga gatggtgtta agttggttga aataatgatt tttttaccag
    36001 caacaagtct tgtatttaca atattgaaga tttctccact agtaaaagaa ttattaagtt
    36061 cagttccaag gtcatctatt attaataaat cacaatcaaa caagttttta taattatctt
    36121 cacttatttg attatttgtg tctcttctaa atttataatc ttcaagaata tctaaaattc
    36181 taaaagaagt ttgatagatt acaacattgc ctttatctaa caattccttt gcaatacaat
    36241 tgcacatata ggtctttcct agtccagtag aaccataaaa cagtagattt tcactattat
    36301 ccttcttaaa atcatgaaca aaattttcgc agatacttaa attatttagc atattttctc
    36361 taggagatat ttcaccatca gagcctttct taggagaaaa tatattcaag ttaaaattcg
    36421 aaaaattttc ctgacttaaa atcctatcta aattagacat tttataagct tcatttacaa
    36481 tttcttgttt gaggcaagaa catttttctc cattaggtaa aaatccttta tctttacaag
    36541 aaatacattg aaactttatt tccaagtaat caagaggtat gttactttca gctaaaagtc
    36601 tttctttctt tacttttaaa gattctatat tctctttagt cttctttaca atttcatctt
    36661 tagacttagg atttaaaagt actatcttag ctaaactgag acctatttta gaaatttcat
    36721 catctataga ttttatctca gggattctat tatatacctc attttttcta tgttctaata
    36781 agagttcatt atcatctctt ctcttagcat atttagcaag tatttttctt attttatcct
    36841 cattcattta atcacctact taaacttttc tttttgactt tttttaataa tctcatcgag
    36901 ctcatcagag gtatactgag tgaatgtttc gttgaaatta tgaaacttag tttttttgta
    36961 agtattgttg ttttgagtag tattaatctg cttcttatta atattctcct taactatacg
    37021 ttcttcctct ttttgcttta aatcatttaa attttttata tttttttcat tccaattttt
    37081 aattatacca tttatataag atatacttgg gtttgaaata ttcttagact tagaacatgc
    37141 atttattatc aaatctatat ccatattaaa tttatcaaac caggtgtcca taagttcttt
    37201 ttctgatttt gatggttgtc ttgaaaatcc caattcattg aaaatcgttt tatataaaat
    37261 atatcgttca cttcttacca aaaagctttc ttcaacatct tttggcgtat ataaatttga
    37321 atcataccaa tttctaataa tgccttcaat gtatttaact ggctttgatg taccagtttt
    37381 atctttgacg tattcatatg cgcaaagtat catatcgggg ctcatattgt atttattcat
    37441 tatatccata atactcatct tttcacttgg gtcgagatat cgcccaacta ttttatttat
    37501 agaattaaac atttttctaa tacttggatt ttcagaagta gatacaactt tatctgtatt
    37561 agattttata gatgaattat tgcttaatat attctcaata tagaaatttt ttaaatctaa
    37621 aaattctatt gaataattaa aattatcata ttcttcattg tcatgtattt ttataatctt
    37681 cttttcttcc cagaatttcc aagcagataa cacatcagac aatggtatat ttaggttttt
    37741 ggctattgaa ttattgtcga acttaggatt agaagtaata tcacatgctt gtctatatcc
    37801 taaaagataa acttttacgt ataacccgtc cgccattggc ataaatatat ctataaagat
    37861 attgggaata gtgatttcac ctaaatctat ttcattgctt tctttaaaaa acattttatc
    37921 acctcagatt aaattatatc attttttata ttatatttgt ctgtaaacaa ataaaaggtc
    37981 acaagattag catgtaaata ttttctaaaa gtattcattt tgaaaaggag gagatatttt
    38041 ggttataaaa ttcaaccaaa aagtaagaag aacattatca gtagcgattc tatttatggt
    38101 attaataata ggttgcttta tagaaaagga tgacagttta gaaacataca aagaaaaaac
    38161 taattcaaaa ataaatcagt ataatatgga tgttatattt gatgatgaga ctaagaggtt
    38221 aatgtgcaat cagaatgttg actacataaa taatacaaaa tcaaacatag ataaaatata
    38281 tttccatata tatccgaatg ctttttccaa gaaagatttt gctccttttg aaaaaagtga
    38341 aatgacaagg gcttatccta atggatttaa cgaaggatat atagatataa agaatatatt
    38401 aaataataat agtaaaatgg agtataaaat taaaggagac aaaaatgaca tattagaaat
    38461 agaattaggg aaagagttaa aaccaaatga aagaatgtca ttagatataa aatataacgt
    38521 aaaaatacca aattcaattg gaagatttgg atatggagaa aatactataa atgttactaa
    38581 ttggtttcct atagcttgtg taaatgatga aaggggatgg aatttaaaga gttatgaaac
    38641 tattggcgac ccattttata gtgagactag cgattttcat gtgaaactat tgattccaaa
    38701 taaatataaa attgctcata caggaaaact tattgatgat aaacatgata ataagaaaat
    38761 gctatatgaa atagaagctg aaaacgttag agattttgca tttatactaa gtagtaaatt
    38821 tgatgtaaac aaagtagatt caaagggagt agctatcaat acatataatt taaataaaaa
    38881 gctttctaaa aaagcaactg aagtggcaaa agattcaata aatatattta gtgaattatt
    38941 tggaaagtat ccttacaaag aattttctgt tgtagcaagt gactttttta taggggggat
    39001 ggaataccct atgcttgtta tgatagacca aagtttatat aatgaaaaaa atgaattttt
    39061 actagaatat gttattgccc atgagacagc acatcaatgg tggtattctg ctgttggaaa
    39121 tgatgaaata agtgaaccct ggttagatga agcacttact gaatattcta caatagttta
    39181 ttttgaacaa aagtatggta aagatatggc aaacaagctt ataaaaacta tggagataca
    39241 gacaaaaagt tatttgagtg aaaatatatt taagccagca aatgagtata aaaattctac
    39301 tgaatacagc ttgaatgttt atacaaaagg cgcaatagca tttaatgaaa taagaaaaga
    39361 agttggagat aaagtattct ttgagacttt aaatgagtac tataataaat ataagcataa
    39421 aaatgctaat ggaagagctt ttgtggaact atggaatagt aaaggtgtag atataaataa
    39481 gattataagt gaatgtaagt aaatagtgaa tgtaagtaaa ttttgtgcag agtattaaat
    39541 tttatttaat actctgtttt tttggtataa tataatttat aaggcagaaa aagaggtgaa
    39601 ggaatgctaa agtattgttc agtagggagt ggtagtagtg gtaattgcca ctatataggg
    39661 tataaaaata ctaatatact agtagatgca ggattaagtg gcaagagaat aactacaggt
    39721 cttaaagaca ttggtgtaga tgcagataaa ttaaaaggaa tatttataac acatgagcat
    39781 gttgaccata taaaaggagc aggtatatta tcaagaaagt ttgatatacc agtatttgct
    39841 aatataaaaa catggtgttc tatgaaggac aaattaggtg acatcaaaga taaaaatatg
    39901 aaagtatttg aaaatgataa aacctactca ttaggtgata tcatagtaag acctttttca
    39961 atagaacatg atgcgtctga cccagttgga tataattttt atacagagag tgatgaaaaa
    40021 atgtctatag ctactgatat aggatgtata actcaaaata taaaaaaaca tctatataaa
    40081 tctaaattag tagtattgga atcaaattat gaccctaata tgcttatgat gggttcatat
    40141 acatatgctt taaagaaaag agtaatgtca agtactggtc atttatccaa tgaagatgca
    40201 gctaatttct gcgttgaact tattaatgaa ggtacagaat ctatattatt agcacattta
    40261 agcaaagaaa ataattttcc tgagttagcg tatgagactt caaaaggagt attagcatca
    40321 aatgatatag tagttggaca agatgtaaaa ctagatgtac tatcacgaaa tgatgtttct
    40381 aatgtttatg agatgaagta gaggtgaata actttgctaa agagaaattt tgaaaatgtt
    40441 gaggataata aaattttata ctctataaag cagggtatga ttttagcgat acctgctatt
    40501 atgactggtt caactgctct tgtaattttg aatcttccaa taaaggctta tcaagaatat
    40561 ttatcaggct tgtttaatgg agaggttaca aatattctta actttataaa tgattctact
    40621 ttaggaatta tttcattaat aatattatta acaataagct atagttatgg aaaaatatat
    40681 gggagcaagt atacagtact tgttcctata gttgcaatgt gctctttttt agtattttca
    40741 catggtaatg agcttaattc ttatatagaa atatttaaaa caaagtggtt atttacatct
    40801 ataattgttt ctatgacatc ttctgtattg tttgtaaaac ttacagaatc ttttgttaca
    40861 agtgtaaagt ttcatacaga aggggctgat gcagacttta atatggtagt ttcggctata
    40921 gttcctttca taatagtagt atttttgttt tctattttta gagtggtaat gataagtcta
    40981 attggcagtt caaactttca agatatattt tataatttat tttcaaatgt ttttaataaa
    41041 atgggaacaa accttatgag tgctctgttg tttatttttt taatgcactt tatgtggttt
    41101 tttgggattc atggtagtaa tgtattagat accgtcgcaa aaaatttatt tgaaaatagt
    41161 atggcaatta acataaattt agttaataat aatcaattac caacagaaat ttttactaag
    41221 accttttttg atacaatggt tttattggga ggatgtggct ctttactatg tttagtaatt
    41281 gcaatttttc ttagtgaaaa acgtataaat gtaagaaagt tagctaaaat tgcttcaata
    41341 ccagctcttt ttaatatcaa tgagatgcta gttttcggga taccaattgt ttttaactat
    41401 attatgtttg taccttttgt aattactcct ataatcttaa ccgttacaag ttatgtggct
    41461 atgtctacag gtattgtacc atgtacagtt tcagctgtag aatggacctc acctatattt
    41521 ttaagtggat atatgtctac aggttcaata agaggtagtt tgttgcaagt atttaattta
    41581 tgtgttggtg ttatgattta tattccgttt ataaaaatgt cgcaaaatag atatacatat
    41641 atgtttaaaa ataatataga aaatttaaca aacttattaa aaaggtatga attaacaggt
    41701 gaacagccta atcttctaaa ccataatggt aaaataggtt caatttcaaa aatgttggca
    41761 tcagacttga agtttgctat gaaaagagga gaaatagagc ttttttatca accacaagtc
    41821 aattataatg gaaatgttgt gggagcagag ggattactta gatggaaaca ttcaatagga
    41881 gggttcattt atccaccact agttataatt ttggctaaag aagaagggtt cttagatgag
    41941 cttggagagt atattattaa taaagcatgt attgatttaa agaagagtga aaagctattg
    42001 agaaatcctg ttaagttctc tgtaaatata tctccagacc aacttgatga ccctaagtta
    42061 ccagagcaaa taaaaaatat cattagtagt aatgatataa atcctaatat gttaggtata
    42121 gaaataacag agcaaattgc attatcaggt tcacaaataa ttattgaacg tatcaatgcg
    42181 atacataact tgggaataaa gttaattatg gatgattttg ggatggggca tagctctctt
    42241 atatatcttc aaaataataa ctttgatata gttaaattgg atggttcatt agttaaagag
    42301 atacttacaa ataaaagaag tagtgaaatt ataatgacta ttgtaaatct ttcaaaaaat
    42361 ttagattttg atatagttgt tgagtatgtt gaaaatatag accaaaggga taagttatat
    42421 ggtttaggat gtgaaatata tcaaggatat tattatagca aagcaatacc ttttgaagag
    42481 tttgtagaat atgcaaatca agaaagagag atataatttt ataagagctt gtaaattcta
    42541 gtaatgcata gatttgtaat aaaattaagt ttaggagaaa tatatgaata taagtatagt
    42601 agtagtaggt aaaattaaag aaaaatattt aaaattaggt attgatgagt ttaagaaaag
    42661 gctttctaag tattgtaagt tagaaattat agagttggat gatgaaaaag ctccagagaa
    42721 tttgagtgtt aaggaaatgg aaattataaa agacaaagaa ggaaagaaaa ttttagggaa
    42781 gataaagcat aatagttatg ttatagcctt ggctattgat ggtaagggtt tatcctctga
    42841 agagcttgct aagactatga gtgatttagc agttagaggt aatagtagtc tctgttttat
    42901 aataggaggt tctttagggc tttctgatga ggttttagga agggctgatt ataggttatc
    42961 cttttctaag atgacttttc ctcatcagat gatgagattg atattgttag aacagattta
    43021 tagggcttat aggataaata atggagaacc gtatcataaa taaatgatac ggtttttgtt
    43081 aatttgacac ttaataatat taaaataaca aaaaagtatt gaaagtatat tgcatttaag
    43141 gtagaattaa ataaaatatt ataacattat aatgtttata tggttgttaa taaaaatttg
    43201 gaggaaaata tatgataaaa ataatgttgg cctgttcagc aggaatgtct acaagtttat
    43261 tagttactaa aatggaaagt gcagctaaag aaaatggtat agagtcgcaa atttgggcaa
    43321 taccagaatc aactatacaa aatgaaatag aaaaatgtga tgtattatta ctaggaccac
    43381 aagttagata tgtattacct aaggctcaag aaattgctaa gccatataat ataccagtag
    43441 aagttataaa tatgatgcac tatggaacag ttaatggaga agctgtttta aatagagcga
    43501 tagaacttaa caacactaag taatataaat tataatgtat ttttaatata taaattggga
    43561 gaatcaaagg atggatgaaa agactataga aatatctttt aatataatag gttatgcagg
    43621 tgaaggtaaa ggattagctt ttgaagcaat aaaagaggct aagaatggaa atatagaaaa
    43681 agcaaaagaa ttattaaaag agtctaggga agttataaat aaagcacata gatatcaaac
    43741 agaattaata caaaacgaag ctagtggaaa caggactgaa ataagtgtca tacttataca
    43801 tgcacaggac cacttaatga atggcatgaa ttttcaacag ttagctgagg aaatcgttga
    43861 tttacatttg aaattacaag ataaataaaa attaaagata ataaattaag agagttctta
    43921 aaagatacat tatgtgtagc acactaattg ttagattatt gattcaatat ttagtgagta
    43981 atacattagg attattttga ggactctttt tatatttagt ttaaagttaa taaatattta
    44041 ttattaataa tataagatta tatgttataa tattataaca tataatttaa gaggtgaatc
    44101 atgttaagaa atcaaccatt atatatacaa gttgtagata tattaaagca aaaaatatta
    44161 aataacgaat ataaaattga tgaaaatatt ccaacagaaa gggagttaga gcaagagttt
    44221 ggagtcagta aaattactat tagaaaagct attgagatat tagaatcaga aggatatgta
    44281 gagaaaaaaa gtggaaaagg gacaaaagta ataagtaata gcctttttaa taagctatca
    44341 aaagcagagt ctttttcaag tatactttta tctaaaggtc ataagttatc taaagagact
    44401 atttcaattg aaaaaataat aaatgataca aattcacaag tatttagtat atttggagaa
    44461 aaatgtagca agattactag aatgtattat ttagatagta agccatatat attttttact
    44521 cattatattc cttttgatga aaatctaact gttgaaagtt ttaataatga aagttcttta
    44581 tatatgtact tatataggag aaatataaat ataactagat ttgaagatga attttttata
    44641 gaagaatcag aactagaggt atcaaaggct ttaaatctag ctaaagacat attattagga
    44701 agaaaaagaa agacctatga tgagaatgat aatatagttg agctatcatt tgcaagatat
    44761 aatacagaag tacaaaacta tgtaattaaa tacgaaatat aataagaatc ctgagtttat
    44821 tgaaatgata atatgctcag gatttttgtt tagttgtaaa aataaaaaat attataacat
    44881 tataatgttt acaaccaaac attataatgt tataatattt ttaataaaca agtgatatta
    44941 tcaattacat aaaaattagt ttatgttatg aaatcgttat tgcattataa ataaaggata
    45001 atatcaataa tacaggggga taactatgga aaaattcgca agttttttag agcgcaagat
    45061 tatgcctgta gcagcgaaga taagtaatca gagacatatg agagctgtaa gagcaggtat
    45121 aatagcaaca ttaccactaa caatagtggg gtcatttttc acaatattat taaacatacc
    45181 tattgatgct tatatggatt ttatagctcc atttaaagca atattagata taccatttag
    45241 atatactgta ggatttttat ctctatatgc tacttttggg atagcatcat cacttgctaa
    45301 aagctacgat ttagatacaa caggtgttgg tatgcttgca gttatggcat ttttagtttc
    45361 tacaatagtt ccaactcaag tagttgaacc agtaggtgga gttatagaag ctggtagatg
    45421 gatggatatg gcaaaactta attcgccatc attatttgga gctatcgtaa catcattaat
    45481 tacagttgaa atatatcgtt ttatgaagga aaagaatatt acaattaaga tgccagcagg
    45541 agttccagta gaggtttcaa attcatttac tgcattatta ccgacagcag taattttaat
    45601 gttattctgg gtaataagac atattctagg atttgatata agtgttgcac taagcgctat
    45661 gttatcacct ttaaaaggat tcttatcagg aaatagttta ggtggtgggt tactgacagt
    45721 attcctaata actttattct gggtacttgg aatacatggt ccagctataa tgggaccagt
    45781 aataagacct atatgggaca tgtctatagc agaaaatata actgcattca cagaaggagt
    45841 atcagctcat gcaatgccaa acatatttac agaacaattt ttacagtggt atgtatggat
    45901 agggggagca ggggctactc ttgcattagt tgtaatgttt atgttttcta agtcacaata
    45961 tctaaaaagt ttaggtagat taggattttt accagggtta ttcaacataa atgaacctat
    46021 aatatttgga gcaccaatag ttatgaatcc aatactaggg ataccattta taattggacc
    46081 tataataatg acaattctat catatgttct tactattaca ggggttatac ctatgatggt
    46141 agcaagatta ccatttgcaa tgccagcacc aatagcagca gtaatgagta ctaactggag
    46201 tatactagca ggtatcttag tattaatcaa ctttgtgata tcatttgctg tttattatcc
    46261 attcttcaaa gtttttgaaa aacaacaatt acaaagagaa caagaagaaa tgagtgaaaa
    46321 tttataaaaa tacatagata tagaggtttt gtactatata aaatatagaa aaagcttata
    46381 agttattaag ggtgaaggta ggaggaatga cctttgcctt taattttata tattttaaat
    46441 aatgtaattt gaggaggaga aagatggaaa gaagattagg aatatcagta tatccagagc
    46501 atagtaacat ggaagatgat aaggcatata taaaaatggc ttcaaaatac ggatttaaaa
    46561 gaatatttat gtgcatgctt tcaattacaa gacctaagga agaagttaaa aaacatttta
    46621 aagagattat aacctatgca agagaattag gatttgaagt aatccttgat atagctccaa
    46681 acatatttga tatattacaa atatcttatg atgatttaac attttttagt gagttaaatg
    46741 ctagtggaat aaggcttgac tatggatatg atggaagtaa agaagctatg atttctttca
    46801 atccatatga tttaaaagtg gaactaaata tgagtaatga tgtagcatac ttggataata
    46861 taattactta caagccaaat gtaaataata tatatggttg tcacaatttt tatcctcaaa
    46921 ggggtacagg cttgccatat gatttttttg taagatgttc aaatagattt aaaaagtatg
    46981 gaataagaac tgctgctttt gttaattcaa aatacgcaac aatagggcct tggaatataa
    47041 atgatgggct gtgtacgctt gaaatgcata gaaacttaga tattgctaca gcaaccaagc
    47101 acttattcgc aacaggattg attgatgatg taattgtagg gaatgcatat gcaagtgaag
    47161 atgaattaaa aagaatgtct gaagtaaata aatatcaagt tgtatttaat gtagtaactg
    47221 aagaaaaaac ttctgaagta gaaaagacaa taatggcaaa tgagcaacac tttagaagag
    47281 gggatataac caaccaagta gcaaggtcta cagaagtaag aaaaaaatat gctaaagaaa
    47341 atttccctaa gcatgataat acgaccatct ttaaaagagg agatgtagtg attggtaatg
    47401 atgactttgg aaagtataaa ggggaattac agttgatact tcaagattgt gcagatgaaa
    47461 gaaaaaataa ggtgggtaga ataaaggaag acgaaataat acttttagac tttattgaac
    47521 cttggagtaa atttaaactt attttagagg gagagtaaag tgaagataga catattaata
    47581 aaatgtggaa aaacagtaga taaaagtata attgatatag ctatattaga cgataaaata
    47641 atagaagtaa aaaatcagat agatgattct aagtatcagg caaatcaaat tataaaccta
    47701 gatggagaaa aatttattag tgctggttgg atagatatac atacgcactg ctacgaaaga
    47761 ctagaccttt ataaggatta tccagatgag gttggaataa aaagcggtgt tactactgta
    47821 gtagatgcag gaacaacagg agctttagat ataggtaaat tttatgaaga tactaaaaaa
    47881 tataaaacaa atgtatatgc tttaataaat attgctaagc agggaataac atctcaagat
    47941 gaactttcaa gtatgatgaa tattgatgaa tatgaactta gaagagcagt taaaaaatac
    48001 aaagattttg ttgttggaat caaagctaga atgagtaaaa gtgttgtaat ttcaaatgat
    48061 gttgaacctt taaaagtagc taaaagaata aaaaatgaac ttaatctacc tatgatggtt
    48121 cactttggga gttctcctcc aacgatagag gatatatttg actatatgga aaaaggagat
    48181 atattaaccc atatatataa tggaaagcca aatggaatac ttagaggcaa tgaggttaaa
    48241 aaagaaatca tagaagctag agaaagaggt ataatccttg atgtaggtca tggaaccgaa
    48301 agttttagta tggatattgc aatgaaatct aaggatgcag gaatttttcc agacactata
    48361 agtacagata tatatattaa aaatagaata aatggtccag tgtataatct atctacaaca
    48421 atggaaaagt ttatatatat gggctattca cttgaagata taatagataa agttactaaa
    48481 aatgcagcag atgcaataag tttaaaaaat aaaggtttaa taaaagaagg atatgatgct
    48541 gatttaacta tatttgatgt ggttaatgaa gaaaaagaac ttcaagattc tttgaataag
    48601 tcagtaatca caagtacgtc tataaagcca agagcagtag tagtcaatgg agaatattta
    48661 aatataggta agtctatagg agagaatgag tagaagggag aaactattgt atgagtagta
    48721 tatttgaaaa atactctgta agacgtgtta taaatgcttc aggcaaaatg actatactag
    48781 gagtatcaac agtagataag gaagttattg aagcaatagg tgaagggtgt aaaaatttct
    48841 ttataataga agaacttata aataaaacag gtgaatatat agcaaattta ttagggtgtg
    48901 aaagtgcact aatagtttct agtgcatctg ctgggattgc acagagtgtg ggagcaatga
    48961 tagcaaaaga tgatatgagt ctagtttaca atgtaaataa tcctaagaaa caagttaaaa
    49021 gggaaataat aattccaaag ggacataatg ttgattatgg agttccagta gaagtaatga
    49081 taagacttgg aggaggagaa gttgttgagg ccggttatgc taatatgtgt aacaaggaac
    49141 atataataag agagataaat gaaaatacag cagcaattat gtatattaaa tctcatcatt
    49201 gtgttcaaaa gagtatgcta agtgtattag atgcagtaca agttgctaaa gaatataatt
    49261 tacctttgat agtagatgca gcagcagaag aagatttaaa tgtatattat gaattgggag
    49321 cagacctagt gatatacagt ggtgctaagg ctatagaggg tcctagctca gggttagtaa
    49381 taggtaagaa aaaatacata gataatgtaa agcttcaatc aaagggtata ggaagagcta
    49441 tgaaaatagg aaaagaaaat attgtaggtc taacatgtgc aattgagaga tatataagta
    49501 atgataaagt aactttaaaa gaaatggagg ataagctaaa tccatttata gacaagataa
    49561 atactataaa gggagtgagt gctagtataa ctagggattc agcaggtaga gaaatacttc
    49621 gtggagaaat tgactttaat gaagatataa taaataagtc aacacaacaa attataaatg
    49681 aacttagaag tggagagata gctatataca ctagagacta taaggctaat gaaggcaaga
    49741 ttgagattga tataagaagt gtaactggaa gtgatttaga tactatttat aataggataa
    49801 aaaatatagt agaaagttag agtgtagagt aagtgacatt ataatgattt aataaattaa
    49861 attttaccaa tggaggggat aaatatgaat tgtataccac aatattataa aaatagagtt
    49921 tgtttaaatg ttcttgcagg aagcataaaa aatgctaaag aagtatatga tgcttgtgac
    49981 gggcatgtat tgataggaat actatcaaag aattatagca ctgtagaagc agctattgaa
    50041 gatatgaaaa aatactcaaa ggaaatagat aatgcaatat ctgtggggct tggagctgga
    50101 gacccaaatc agtcaaatat ggtatctcag atttctaaag aacttcagcc acaacatata
    50161 aatcaagtgt ttacaggtgt ggctacgtct agagcattgc ttggtcaaaa tgatagtata
    50221 ataaatggat tagtttctcc aactggtaaa ccaggatttg taaaaatatc cacaggtcca
    50281 ttaagttcaa aagaaaaaga tggtatagtt cctatagaaa cagcaatagc tatgatgaaa
    50341 gatatgggag gaagctcaat taagtttttt ccaatgggag ggcttaaaac aaaggatgaa
    50401 tatatagaag ttgcaaaagc ttgtgcaaag cataattttt atttagaacc aacagggggt
    50461 atagacttag ataacttcaa agagatagtt caaattgcac ttgatgctgg tgtagaaaaa
    50521 gttatacctc atgtttatac atcaataatt gataaagaaa caggtgaaac gaaagtggaa
    50581 gatataggag tactttataa aataatgaaa gaattattgg gatagattta tgaatatatt
    50641 agcttttggt gaaataatga tgagactttc agtacctgac tacaagttct taacacaaac
    50701 caatgaacta aattatataa ctacaggcac gggattaaat atattatctg gacttaaaaa
    50761 ctttggatac aatacctata tgttgacaaa gttgcctaat aataatgtag gtaaagcatc
    50821 aagtgcaaat ataagaaagc ttggagtaaa agatgacttt atcacgtatg gagggaatca
    50881 tataggagtg tattttcttg aaaatgggta tggagaaaga ccttctgaag tgacttattt
    50941 gaataggtta aatagttcat tttgtgagag taaattacaa gattatgata ttgataaatg
    51001 tttagaagaa atggatatag tccatatttg tggtattgct atgcaactta cagattcagt
    51061 taaagagatt gctttagaac tagctagaaa atcttatgaa aaggggataa aagtattctt
    51121 tgattttaat tttagacaat cattaaatcc agaaaggact tatgatgatt tagtaaatga
    51181 ttataagaaa atactaccat attgtcatgg gctgtttgct agttatagag atataaataa
    51241 gatacttaat atagatagtc taaatataaa atctagttca gaattgatta aaaaagagta
    51301 tgatatagat attgtagctg gaactatgag aagatttgat gatatgaata atagatttat
    51361 aaagggatat atatcaaatg atgatggtta ttttgaaagt agggagtata agctagaaat
    51421 atatgacagg gttggtgccg gagatggata tgtagcagga attatatatt catatttgga
    51481 aaatatgaag agtgaagata tagttgagtt tggggttgct agttcggtgt tagctcatac
    51541 aacttatggg gataattctt tagtaagcac aggtcatgta cttagactga tgaataatga
    51601 aaaaatagat atattgagat aatacatttg tatataataa aatttttatc ttttttagac
    51661 acatttttga cttgcttata tttgttaaac agagttattt ttaatgatta taaatattaa
    51721 attaaaagtt aaataataaa atagtagaaa atagcattaa ctatagaaaa aatggttaat
    51781 gctattttta tcgttaatat tgattaattt tatattatag aaatgaactt ttctaatgct
    51841 ggatttgtat tttttttatt ccatacaact acaacttcaa ttctattttc tacttctttt
    51901 ataggtacta ttgcaatatc acgttctatc attgtatctg taacacaaaa tgaaggtaaa
    51961 atggatattc ccatgttaga agataccaat gattcaatag tattaagatt atttgaacgt
    52021 tgtaccacat taggtacaaa ccctgcttca cgatatctat tctgtatcat tgacttttgt
    52081 ccatactcat cccctgtttc ctttacaaaa ataaatttat catgttgtaa ttcaattaaa
    52141 tcgatagaat cctcattgta aagtggatga ccaggaggta aaagagctac caaagaatat
    52201 ctttctacag ttttatattc aaaatctttt aatggttgat tttctggatt aataacaaaa
    52261 gcgaggtcca tagaattatt ttttactaag tttaaaagtt cggttatttc attagtacaa
    52321 atatccatag atatattagg gtatgtatta gaaaattctt ttaaatattg ttgaagtttg
    52381 tttttttcat atcctattaa ggttccaatg ctcaaagttc caaaaaaacc atgtgcaaat
    52441 tgttgtgttt ttgcaatagc atcattgata ttttttatta tagtacgtgc ctcagataaa
    52501 aaaacttttc cagctggtgt tagttcaaca tgacgtttac ttcttgtaaa tagagttaca
    52561 tttatttgtt cttctaaagc ttttatttgc tgtgtaacag cagtttgaga aatataaaat
    52621 ttttctgctg ttttggtaaa acttagattt tctgctacag atataaaata ttctaattgt
    52681 ttagtattca aataatcacc cctatttaaa cttgattgtt tgtaaacctt atttatctat
    52741 aattaaagct tattatagta taagaaaatt tacttttaaa gaatattaga aaatatttgt
    52801 ataaaaatat atgttttaag tatatcttta cattagattt tataaattta ttaataagaa
    52861 taacttatat attccaatga aaacgatatt ggatttaagt aagtttgtct cataaaatga
    52921 aagtatatca attgataata ctttaaataa gaagggagaa aaaatggaag ataaatttta
    52981 tgcaaaaggc aacggaaata acggatatat taaaaatctt gaagtttgtt cctttaataa
    53041 cttagatgga acttgtggaa tgtttcaaat ggctctgtac aaaagagatg aaaaatacta
    53101 tttatatgga tgctgttttg gaggaaataa aaaaaatgga gtaatgatta gcgatattac
    53161 agacccttat aatccacaat ttataaaaca ttttcaaatg ttagacccta aagagtatcc
    53221 tacaacaaca actcccaaaa ttcaaatagc agatgattta atgatagtag caatgagttg
    53281 tggaagtgga ccaggagcac ttgttgacca agctaaatta gcaaatatta agtgtgaagc
    53341 aggaattaga atatatagtt taaaagaaga ccctttaaat cctaagtttt taggatattg
    53401 ggattgtggc ttaaagcatg taatgggtgt tcatagattc atgtacaatg gtggaagata
    53461 tgtacattta tcaagtgatt gtgttggctt tgaaggtctg atttataggg tcatagatat
    53521 aataaatcct actaatccag tggaaatagg taaatggtgg agaccagacc aatatgcaaa
    53581 tggatatcca aatagaactt ttgatgcagg agcacctcat tgcccagaat ttatggataa
    53641 aggatggctt catggacctc catttgtaag agacggaaaa gcatattgtg gttatggagg
    53701 agctggttta gttgtattag atgttgaaga tttaacaaga ccaagatgct taggtgaatt
    53761 gccatttatg cctgcatttt ctagtagact tgcaggtgca agaactcata cagcattacc
    53821 attgccagga agagatttag tcgttgttca aaatgaggga gaaagattcc agttctttaa
    53881 accagataac attacagatg ttcaagctat gaataatata catatggttg atgttagtga
    53941 cccaacaaaa ccaacattaa ttgctcaatt tccatatcct gaagttccaa aagatttccc
    54001 ttatcctaac tttaatgttg cgggattagg aaaaccaggg ccatttggcc cacataatct
    54061 tcatgaacca atggataata agccatggtt agagcaaaga ggagatagag tatattgctg
    54121 ttatttccat gcagggctaa gggtttatga tgtatcagac ccatattata tcaaagagct
    54181 agcatatttt ataccaccaa atccaaataa aacaccagaa gaatcttatt tcccaggatt
    54241 cccaggacca cgcttggcag taacagaaga tcttatcgtt gatgatagag gctacatcat
    54301 catagatgct ttagatgatg gattctatat attaaaaatg aaagaagatt aaataaatac
    54361 taaagaggga gagttgaaaa tgaaaatact aggtatttct tttggaacaa aaaatggaaa
    54421 taatgacaca atgtgtaaag ttgcacttaa aggtgccaaa gaagcagggg ctgaagtaga
    54481 atttattcaa atgtcatcta ttaacattaa acattgtact ggatgttgtg cgtgtgttaa
    54541 aactttatta tctgggaaag gtagtatgtg tgtattaaaa gatgattttg aatggttgct
    54601 tgacaaaatg aaagatgcag atggtatagt agtttcagac cctatttttg aagaaggggc
    54661 atcaggatta ttccatacta taatggaccg ttttggaccc agagcagata gaggaaataa
    54721 cattatagga acaaaagttg cagaagctat aggaggaaaa attccagatt caagaatgct
    54781 taatgataaa gtaatatcat ttatgggaat aggaggttct gactggggta caagagttca
    54841 atgtgaacat gctatgctag ctctcatacc aatgtggaaa gttatagata atgcttggtt
    54901 cccatgggca aaggaattag taatggatga tacaaggctt gttcaagttc atcaaattgg
    54961 tttaaatata gttgaagcag caaaagattt tgaaaaagct tcatatcaag gagaagaagg
    55021 tgtatgtcca cattgccaca atagattatt ctaccttgag ccaggtacta aaaaggcaat
    55081 ttgtgctcta tgtggtatag ttggagagtt agatactata agtggaaaaa ctatatttag
    55141 ttttccagaa gagcaattag gtcatgctca tgatacttta ccaggtaagt ttatacatgg
    55201 tgatgatatt aagaaaatgg aaggtcatta tgcagaagtt cgtaaaacac aagaatttaa
    55261 agaaagaaaa acatcttata attttataga gccattaact aaagaaaagc aagctggagt
    55321 ttcattagga taataattaa aatactaaga ctctttttta aggtatgtta gatgttatag
    55381 gaggcttatt gtatgaatga aaagtcaagt tttaaacaaa ttgaaattat tttagcagtt
    55441 atttttttgg tacttacata ttacaaggtt agattaactc cagcatttat tgttatattt
    55501 ataccactat atttaagtta ttttgcagta tctatgattg taaatcaaat agcaattaag
    55561 ctattggaga taaggcaagc tagggaagct actaattaca atataggaaa tggatagaaa
    55621 gaaaaaagaa atattaacac tgtctatggg gattgcttta ctacctcctt tatgggcagt
    55681 attagcacct tatataggaa taaaaactgg agctgtagca ctaatctgtg caggattata
    55741 tgtaactaat ggtaataaac aaaaagatgg attaaaaata atgtttggtt tttggtgtgg
    55801 agatttatgg gcagtgttag ccattttaat tatgggatat atgaatttca atcaaaacct
    55861 agaattattt ttaacactta gtatattagg tttttttgca gttgtaattg catccttatt
    55921 tgaaaaaata attttcctac catcatggct ttgtggttgg gctataggtc taactattat
    55981 gacaggtgaa aatattatta atttacaaga tatttgtatt caaattgctg tagctatggc
    56041 agtaggagta tggtacgtgg gtgcaggtgt agatttgttt acaatatata ttttgagtaa
    56101 agataggaaa aagggaaatt gaaagcatgt gaatatactt tgattatagg agaatggcta
    56161 tgggttttga atttaaaata atgagaagtt taatatatgt aggacttgcc aaggaagaat
    56221 atagacctaa gctaatggac tggttatatc gtcaccatat tccagatagt attagcactt
    56281 ttggaccata ttgtactaaa tatgcctttt atcaagcata tcctacacca aatgaaggtg
    56341 agcgttttgg tgcacgtaag atgcaactaa cagaacatta ttggcttgta gatgaacata
    56401 tgcctgagat ggcaaataga attatgacag aatatatgcc tatggatgtt ctacgttggc
    56461 aagggtgtat accagatgta gaaaataaaa gggttcatga aaatgcagaa agtggagatg
    56521 caggacgtgc agtaggtgga gataatggat gtccaccatt tatatttgcc tttgttccaa
    56581 taaactggga agaagacttt agaggaaaag gacgtactgt acaagatgga ccaaactatc
    56641 gttggcaatt tatgattaag tatccagatg gtatctctaa agaagaagga gaaaaatggt
    56701 tctatgatga ggtagtgcca tactttacaa actgttgcta tgttaatcgt tttgtcagta
    56761 gtaaaataat gattaattat ggagcaactg cttttgaccg tgtatcagaa ctatggtttg
    56821 aaggggaaga agaatggtat aaagctgtgg ttgaagagac aaagtcgttt attaaaaaac
    56881 cagaatgggc acaagaagag gagttcccat atttaaaacc acaattcaat atcgcatcag
    56941 tattcttagg tgatatagca actatggatg catactcaca gtatcgtgga tatataccaa
    57001 tgagataaaa attatagttt gtgataagta gaggaggcat ttactcttac tggtagaaga
    57061 agacttattg tatccttaaa agtaggtgaa tatatagttt tatatttaaa tttatataaa
    57121 actaagacta ataaatatga aacaatagaa ttagaataat ttgataatat ttgttaaaag
    57181 agcatttaga atgtgtttaa agattaagat attcctttta tggtagcaga tagttgaaat
    57241 aataaaactg ttatcatgaa aggaatattt ttatgtatag gaaattttta aaaataaaag
    57301 ttaaatatgt ttaagaacat cttaaagtga aagaatcatt gttagaaata tgtagacaat
    57361 atagtcttta taacgtatca tattaacaag tttacacttg gattatgaaa taaaaagagt
    57421 aattccaaag gttgtaaata ctatgctgac tttgatactt caaagtttag gtgattttgt
    57481 aattgttaat agaaatacgt ttaatgagca taaagaattt atctaataat aggagaatat
    57541 aatgtataca attggacaag tatctaaatt tttaggaata tctagagata cactaaaatt
    57601 ttatgaggac aaaggattag taaatcctaa gaaaaatgat gaaaatggat atagaatata
    57661 taatcaggtt gatatctatg atattgcaac aataaacttt tatagagaaa ttgatataga
    57721 aattaaaaag attcaagaaa taagaaaaag taagagtata aacaatttag aactcttact
    57781 agaagagaag gaacaaatca tactaaaaga gatagaatat aaaaagcttc tcttaaaaaa
    57841 ggttcagtta gtcaaagaag attgtgacaa aataaaaaca tattttggaa aatacacaat
    57901 aaaagaaatg aagcctttgg aagtaaaagg taaaataaaa catgttacta actatgagga
    57961 atatgaagcc ttaaaagaga atacaaacaa cttaaaaaaa gcagttacat taactgattt
    58021 aagaagagta ataaatttta atgaagaagg tataatagaa gaaaaattta taattgtaag
    58081 ggaagtagat gactacaaca aaatcataga aggtgaagtt ctatctcatc ccaattgtat
    58141 ttatactatt attgaagatg gcagatggtc aactggagga gaaaatattg ataataaagt
    58201 ggaagacaga ttaagagaaa tagcaataga aaatgaatgt gaattacttg gaatagtcta
    58261 tgttaatata ttgcttacta cttatgaaga agggattgag cgtatatttt tagagattta
    58321 tgcacctata aaataatata aaagcattga cctgggactt actcataggt ttaaactcat
    58381 gatataagca gttataactt atatagcaat taaatattaa tttgaaatga ggattactta
    58441 tgagaacaga gaaatcatta aaaaggccta tagccagttt tattattcta acaaatataa
    58501 tttttttacc acttttttta ttggtagtag tgactataat gttaaaactt ccaacagtga
    58561 tatctgacat tgcgttgtgt atatcagcgt ggtcatccac ttttgcattt atgatattat
    58621 ttaaaaaaat ttatccaggt aagaaatttt tagtacacgt aaaagataga tttagaaata
    58681 aacttaaatt ttcaacagta tctataataa ttagtataca agtcctaata gctgtagtag
    58741 ttatatttat attagcatct aaaaatcatg gaataatgcc taactttaca gtttcatcat
    58801 ggggagcatt tatatattta tttttaaaaa atttatttgc aggatcatta ggtgaggaat
    58861 taggttggag aggatttgtt caaaatcatc ttcagaaaag acattctcct ttaaaagcat
    58921 ccataatagt aggtttttgg tgggggatgt ggcatttacc aatatggttt acaacaggat
    58981 tcaaaggact tgatttaata aaatatatta tattttttat gattgcaatt atatcaatca
    59041 gtattataat gacaactttt tataatctaa ataaaacttt gataatacct attgtaatac
    59101 atcaactatt taatttttta atagggataa ttaatggaga tttaatagag cttataaatt
    59161 actatgcaat gttatattta atatttgcaa ttgcattaat agttataaat ccaggaaatg
    59221 tattatataa aaatattaga gagtaatata tatctttatg gtaagatata aagatattat
    59281 tagaagaaca tccaaggagg aataaacatg gttttactga ttgttgatgc acaaaagtta
    59341 ataacaaacg agaggttata taaatttaat gaatttgtag taaatgtaga aaatttaatt
    59401 gatacagcta gaaaaaataa tatagaagta atatatgtac gtcatgatga tggagttgaa
    59461 aatgaattga ctaaaggtaa aaatggcttt gaaatatacg aaaaatttaa accatgtaat
    59521 ggagaaaaaa tatttgataa aaaagtaaat agtgctttta aagaaactgg tctattagaa
    59581 tacctaacaa ataatggtga aaaagatatt attattgctg gtctccaaac agattattgt
    59641 attgatgcca ctataaaatg tggatttgaa catggattta atataatagt tccagcttat
    59701 tcaaatacaa ctgttgacaa tttttttatg tcagcagaac aatcatataa atactacaat
    59761 gaatttatat ggaatgaaag atatgcgaag tgtatttcat tagaggaaac acttagaaaa
    59821 atgaaataaa aatagagtat gaattgtata tgtacataag ttgatttcat aaaaaaggat
    59881 gttaaattcc aagttaaaaa ttagattaaa tatattatat tatagataag gcacatcaat
    59941 tttattttaa attaatgtgc tttttaattg tagtaattta tttataatca atatgatttt
    60001 tattatttaa atcaattttt cttgagtttc tttgtctaaa gaaacttttt ttatttataa
    60061 aatctacagt tatattatag tatctatttt agagaagttt aatttttgta tcaatctaaa
    60121 acaaaagtta tacttaaatg aaattgaaaa cgataaaatt ctcaaataaa ataagtattg
    60181 tggaggtgat gaaagaatcc aatgataata tgaagactaa tcttaataag aaagtagggg
    60241 ttaatggtag tggacaaaga aattgtatca attctccaga ttttattaaa tagcacattg
    60301 attacaacta atggattaca agaggagtct aattcatcaa agagacaaat tacttataga
    60361 ataaataaaa tcaatgacat gcttaaagta aaaaatgtac cacttatatg tttaagagct
    60421 gataaagata ttatcgttag aaaagaaaca aaaaaagcaa ttaaagaaat tttagaaaaa
    60481 aactattcta agaatactta ttattttagt aagacagaaa gacttttata tatgtattta
    60541 atgcttttta ttaacatgga tgatatttca attaatcatt ttataaattc tataaaagta
    60601 agtaggtcta ctgtaaatct tgatttcaag gatttgatac cagagcttga aaagaagaac
    60661 attaaagtta agaacaatcg tataaatgga tattacatag ttggcaatga gatggaaatt
    60721 aggagagtgc ttattaaaaa tattatagaa actttgtcta atgagaaaag ttcaagagta
    60781 tttgatattt ttattaaaga atataattta gatagttttg aagaggctaa aaaaattata
    60841 ttaaagctaa taaaaaaata tgaaattact tttgttgaag atagattagt agaatttata
    60901 tatatattta tctttctaaa ggcaaggatg tattcaacta gaatagttac tcaggaaaat
    60961 gtagatattc ctaatatttc agttatgagt tcaatgaagg agtataaatt tgctaaggaa
    61021 ttattagaag tatatgggag tgaagataag ataaagcctc acaatataat gtatattagt
    61081 gcttggattt taggtatatc agtaggaaat tatgaagaag atacagaaga taaggatgta
    61141 atttcaaaaa ttgtaaataa aatgatgaca aagtttggat atttaagcgg agtttactat
    61201 ataagccaag aaaaaatatt taaacaatta tattctcatt ttagacctgc ctactataga
    61261 cttttattta aattacctat ttataatccg atttgtgaaa aagtaaaaga agaatataaa
    61321 ttagtttata gaattgtaag taatgcaatg aaagagtttt gcggattatt tggagaagaa
    61381 attcaggaag aagagttagc atatcttaca atgcactttg caacactttt ttcaaataaa
    61441 aaagaatttg atggttttag aaaaagaata gcattaatag tttgttcaaa tggcgttggt
    61501 agttcagcta ttttacatgc agaacttaca aacttatttc ctgacttaca ctttatacca
    61561 atgtcggaat cagttggatt agaaaatata agtgaacctg tagatattgt atttactact
    61621 aattataacg cagagggttt aaaaacagat gtaccagtaa ttaaagtaag ccctgttatg
    61681 accacaaaag aaaaatataa gattacacgt gaagtgtata ttcaattagg tgatattttt
    61741 ttaagacaac ctaaaataga tgaggttatg gatataataa aaaaatatgc aaatgtaacc
    61801 tctgaaagta ttctttcaag tgagctatta gcttatttta cacagataga gaattttaca
    61861 tctaaggaag gagaggggcc tatgctaagt gagattacta atgaaacact agtaaaaata
    61921 aaggtaaaag ctaagaattg ggaggaagct attagacagt cagcttctgt attagttgaa
    61981 aataacaaag taactgaagg gtatgtagat gctatgatta actcagcaaa agcatctgga
    62041 ccatacatag taattacaaa acatgttgca cttccacatg ctagaccaga agctgggtca
    62101 aaagagattg caataggcat tgctacttta gatagtccta ttgagtttgg gaataacgaa
    62161 aacgaccctg taaaatatgt attttgtcta agtgcaatag acaataatag ccatttacgt
    62221 gctatgtctg agctagttga actattggaa gaagatgagt tttttaaggt tttagatagt
    62281 gctaaagaag ctaaagaaat tattgatttt ataaaaacac acgaattata aaggaggatt
    62341 taattatgta taaaatttta atcgcatgta gagcaggagt tggctcaagt ttaatgttaa
    62401 aaataaagac tcaacaaatt attaaggaaa ataactttcc aattgaagta gaacatggtt
    62461 cattggattc actaaatgga ttcaatggag atgctgtaga aacattaatt gatgttgctg
    62521 aagaattaaa aaataaaaat ttgaagtttg atataattgg gattcacaat attgttgacc
    62581 gtaatgaaat aaagacagca ctagaaaaat tccttgcttc aaaagaggga taaatttaag
    62641 atgataagga ggaatattta tggagtttat tattagtttg ctgagtacac ctgctgtttt
    62701 gcttggcgta gttgcatgta taggattgat tcttcaaaag aaatcagctg ttgaagtatt
    62761 tacaggctct tcaaaaacac ttatcggttt tttgatattt ggtattggtg cctctgctat
    62821 gacaggagca cttcaaaatt tcaataaact gttccaacat ggctttggaa ttactggtgt
    62881 tatagcttca ccagaagcag ctacagcact tgcacaaggt acatatggtt ttgcagtttc
    62941 atgtacatta attcttggat tcatattaaa tttagtattt gcacgtatta caaagtttaa
    63001 gaatattttc tttacaacag gtcatagttt gttcttttct tgtgtattag ttttaattat
    63061 gaaagcacat ggatttgata atacaatcac tattttaata ggtggaacta tcttagggtt
    63121 tatgtctgct gcactacctc aattctgtca accatttatg cgtgaattaa caggtggcga
    63181 cgaacaagct attggtcact ttaacatgat tggttatggt ttatcaggtt atattgggcg
    63241 tttattttca aaacataaag atgatacaac agagactata gaatttccta agtggttatc
    63301 aatattcaga gacttcttaa tgggtctatc catagttatg ttaatcttat tttatattgc
    63361 aacattaaaa gctggtcaag catttactca agaaattgct ggttcaacac attggttggt
    63421 ttttccgctt atagaggcat ttacatttgt ggcaggtatg tcaatattga tgtcaggagt
    63481 tcgtatgttc ttagctgaaa taactgcagc atttgtaatc atttcagaaa agtatattcc
    63541 aggttcacgt cctgcgctag atgttccaac cgtatttcca tatgcaccaa atgcagttat
    63601 tattgggttt atatcagcat atgctgctgg tctattagca attgctatta tggcatcatt
    63661 tcctagtata tttcctgtag taattattcc agctgctcac atatgtttct tctcaggagg
    63721 tacagctgca atatttggca atacttcagg aggatggaga ggggctattg ctgggtcatt
    63781 tgttgtgggg ttattacttg catttttacc agttatacta tatccagttt atgggactct
    63841 tggtatagaa ggagcaactt tccctaatat tgattacaac gtagttggtt cgatattaca
    63901 taatatttta caatttataa aacctattta gactaaatta tataaaagta tagtcatagt
    63961 tactatgatt tcaaggagga tacatcttat ggaaaataca ttatctgaat ttttagataa
    64021 tatatctaat gaattgtcag aatttattgc ctcaatagat gagacatcta taaataaagc
    64081 atgcgaatta attcttgaag ctgaaaaaaa tcacggacgt gtacatgtga ctggtattgg
    64141 caaaccagga catgtatcgg gatatatttc atcattgctt tcatctactg gaacgtcagc
    64201 atatatattg catggtacag aagcagtaca tgggtcaagt ggtcaagtag ttgaaggaga
    64261 tgttgttatt gcaatatcaa atagtggtga aactcaagag ttaaaagcaa ctcttaagac
    64321 tctaaaagta aatggagcaa agataattgg tgtaagtggt aatgagagtt catttttaaa
    64381 aaatatttct gatatattcc tgtttgcagg tgttaagcag gaaggagatt gtctcaataa
    64441 agctcctcgt gcttcaattt tagctgaaac aataatatta caatcattaa gtgttgtatt
    64501 acaatatgct aaaggtctta atacacagga gtatttgaag tggcatccag caggaagtct
    64561 tggaaagtct attagagagg gaatataatt ttatgaaata taaagggatt atatgtgcaa
    64621 tgataacacc atttgatgaa aatcaaaata ttaattcaca agcaacatgt caattaattg
    64681 attatttgat tgaaaaggga atttatggat tatttatttt gggcactaat ggtgaatgtc
    64741 atgtattaac agatgatgaa aaagttgaat ttgcaaagat tgttattaat catacaaata
    64801 atcgtgtacc tgtttttgtt ggaacagggg gtaattcaac tcgtgaagtt gtagatttat
    64861 ctaagagaat ggaagcgatt ggagcaagtg cattatctat tattacacca tactttgtta
    64921 caccaacaca acaggaatta atcttacatt acaaaactat tgctaattca gttaatttac
    64981 ctattataat gtataatatg ccaggtaaga ctggaattaa tattgaacca gagtcagtac
    65041 gtgaattatc aaagataaag aatattgttg gaattaagga tagtagtggt aaacttgaca
    65101 acatgaaagc ttatattgaa gcaacgaaga atgagaattt tagtgtgttt tcaggctcag
    65161 attcacttat tctggatagt ctaaaagctg gtagccaagg agctgttgca gcaacaacaa
    65221 acttcctcac agagattggt atctctatct atgaaaactt tatgaaaggg gatttggaag
    65281 aagctcaaaa atctcaaagt agtatagaag aacttcgacg tatactgaaa cttggaacaa
    65341 ttccatctgt tatgaagaaa actattgtct tgaatggaat taatgttgga actgcacgac
    65401 ttcctgtaac tgaaccaact ggggaagtac ttgaagaaat taaacgagta gttgaaaatt
    65461 ataggacaat acttaataaa agtagtgaga atagataata taaataggga aattacactg
    65521 gtatataatt aatatatata ttataaaaca tgactaaaat taatgaataa gtaaagtggt
    65581 ttgatgagta tagaggtgat aatatgaaaa agatgaatat aaccaaaaga atgtcagaat
    65641 gtggggctct tgcaattgta agagaagaaa atcttaaccg tgcatgtgaa attgctgaag
    65701 gatgtataaa aggtggtata acagttatag aaatgagtta taccctaaat aatgctggag
    65761 aaataattca agggcttaat aaaaagtatg gtgaaatact ttgtgtggga gcaggaacag
    65821 ttttggatag tgaaacagct cgtcatgcta tattacatgg agcccaattt attattgcac
    65881 caaattataa tgaaggtgta gcgaagattt gcaatagata tcagattcct tatgctccag
    65941 gatgtacatc attgactgag gcagtagacg cactaagttt aggagcaact tttataaaag
    66001 ctttcccaat atcagatttc tatggaccta aactagtaaa ggtatttaag acaccaatac
    66061 ctgatatgcc agttttagca agtggagggc ttaatattga aaacctacat atatggttag
    66121 aaaatggagt tgatgtatgt ggatttggtg gtttattaac taaaggttct gttgaagata
    66181 ttgctaaaaa tgctaacaag attcgtgaaa tcattaaaaa tgtaagaaaa atttaacata
    66241 gggctgtatc atagtatctt ataattacta tgatacagcc tttttaatat tattcaaatt
    66301 taaaacagtt tacatttcta ttatattaca tataatttca gtaattttat cttaattttt
    66361 ctatctggaa aaaaatgatt taaaacttaa gttatgatat aatttcatta aaagaaggcg
    66421 atttttagta aatatgagaa aataatgtgt ttttagggga aaagttaaat acagatttaa
    66481 gtgaacaaga atcgataatt aagatattaa atggtgaata gggcatcaaa aaaattacta
    66541 aattaaaagt tttagaggga gaatcttagc aagtgtgtgt gaaaattcaa tttcaaacaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgata caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tggctatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agagttaaat aaaacaaaga agtccgattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttactcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaatcataa
    72061 tgaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagat ttatcggagc aggcagatag agttataaaa ttgtaataat ataaactata
    72361 ataaatataa gaaagagatt acagctaaat taagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaactaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 tgattttata aattgtattt attattatat cttgattcta aaatcattaa aaatttcaac
    73201 ttctataaag atttacaatt tatcaaatac attatgagta tataaacaat ttattattac
    73261 agtgctacat ttaaaataag tcttagtata aaggtattgt tacaagtata aagaaactta
    73321 tgtatgctat tggaataaat aataccttaa attataatta agaataagaa ctgttaaata
    73381 agggacttga ttatcattta gttatagata atataatatt aaaaataaag tttaagagga
    73441 gaaaaaatat gatagatata cataaaaata tatatgataa taagttattt gaggagctaa
    73501 agattgactg taagaagtgc tttggacttt gctgtgtagc tctttatttt tctgcttcag
    73561 atggatttcc tatcaataag gaatcaggaa aaccctgtat taatttacaa ccagatttta
    73621 aatgttctgt acataatagt cttattaagc gaggttttaa agggtgtact gcatatgatt
    73681 gttttggttc aggtcaaaaa gtagcacaag taacatataa ggggatagac tggatgcaat
    73741 caccagaact aacaaatcaa atgtctgaag tgtttttaat tatgagacaa ttacatgaaa
    73801 tgttatggta tcttaaagaa gcatctgtat taaatatatc agatacaatc aaatcaaaaa
    73861 ttgatttaat aattgaagaa actgaaaaga taactaatat gggcccagaa cagataatta
    73921 atttggatat catatcacat agaacaaagg ttaatttatt attatcacaa gcgagtgagt
    73981 ctatgatggg aaaggttaaa tcatttatca agacttctac tcttaaaaac atgaaaaaat
    74041 taagtaaaaa tatagattta ataggagcag accttagaaa aataaatcta ataggagcag
    74101 atttaagagg tagatttcta atagcagcaa atttaagaaa tacagatttg agtggggcaa
    74161 atttaatagg agcagactta agagattgtg atattagagg tgctaatctt gaaaatagta
    74221 tatttttaac acaattacaa gtaaatactg caaaaggaga ttctagtacg aagttaccag
    74281 cttcactaat acgccctaaa tactgggaaa aataattaaa ttaatacata attttacaaa
    74341 atatgaactt ttttgacttt aattcatatt atacattaga gattctttac aagaatttct
    74401 taatataata tagggagaga tataaaaatg agttatcaaa attataaatg tgaaagagat
    74461 tatgtgtatg atgattataa atacaataag tgcaacaagt gctataatga ttatgaggaa
    74521 atgacacatg ttcatgaata ttcagaaagt gttaagttag cagaagaatg tgaggataga
    74581 cataaccacc gtgcagcagg ggttactggt gaagttatac caataaatgg aggtacaaat
    74641 catgttcata aaataaatga taatgtagat ttccttgacc atttccataa gatatgtgtt
    74701 acaacagggc cagcaattag aatacctgga actgataaac acattcactt aatatgtgga
    74761 gaaactactg ttaatgatgg tcattgtcat aaattccttt ttactactca aatagaagca
    74821 cctttagtat aagctgatgt gaattattgt agtgaatttg ctagattata gtataatata
    74881 taatagataa tatttaaact taatgaggac tatgatattt aaccttcgta gtcctttcta
    74941 agttttgtat aaaaaagtgt tatgcaatta aaagtgatat agggaggaaa tttttatgaa
    75001 atctggaata attcttgaaa tgaaacaaat aaattcaaca gaatatgacg aaatgttgca
    75061 agtctgggaa tcatcagtaa gagcaacgca tgatttttta acagaaaaag atattgaatc
    75121 tttgatacca ttagttgaga ttggacttaa agaagtagaa aatatagttt gtataaaaga
    75181 taatgatata ataaagggct ttataggaat agataaagat aaaattgaga tgctctttat
    75241 agaagataaa tatagaggaa atggtattgg taaaaaatta ataaaatatg ctatagataa
    75301 atacaatgta aaatatgttg atgtaaatga gcagaataaa aaagctgtag gattttatat
    75361 tcacttagga tttaaagtgt ttgataggtc tgaaatagat ggacaaggga acccatttcc
    75421 aatactacat atgaaattaa tttaaagtta tacttcccac ttattcgcaa aaatatttac
    75481 ttaaattttt atacaaataa aagtcgtgac agtcttagga cacgactttt ttactacact
    75541 attaaattga aatttatctg ttctatatta tgattattag atgtagtttt tttattttaa
    75601 ctatgtactt aaataccaag tatacccata cctatcaagt acttcagcag cgccatcatt
    75661 ttgatgtggt ggtgggtgtg gtaaaaattc ttgtacaagt gtaccacctt cggcaagttt
    75721 tacagatatt tcttctatcg cttctcgaga tggtagaaca acacaaatcg ccatgccaga
    75781 aattacaaca ggcttgtctg tatccgcacc cattatacaa ccttcgtatt ttgtgaaagt
    75841 aacacttgcg tgcataatag gattgtttgg attgctgtct aatcgcgcaa atatagtttt
    75901 tcctcctccg aaagcctcag cataaaaatt aaatgctttt tcacaagtac cattaaatat
    75961 taaatatgga gttagcatga ccaatatatc ctccttgata tagaataatt tatagttgca
    76021 cctgataatc taaattatga tttagtaaat tgtatcatag ggaaaccttg aagtatataa
    76081 ttttttatta ttatattata caaatttatt aatttaaaaa aatgttatat gtacggaagg
    76141 tttacaggat aaaatataat aaaaagatta aggtgacatt ataaaaaatg tatattgaca
    76201 ttgacgctac gtaattgtat atactttgtc ttaaaggaga tggtaaatat gaatattata
    76261 gaaattcgct cagataaaat atacaagaag ataatggatg caccaataaa caaaaaagaa
    76321 gatatataca gatatgaatt aatgaagcct tttgaattta agtggaagtg tatgaatgtt
    76381 ccaatagttg ctagacagaa aggtggatat gatgtaatta tagcaagtga aatgttaggg
    76441 gttttatcgc ctaaggatat tgatgaaaag caaaaaaaga atataaatgt gttatctgct
    76501 gataaaattt ggggcacttg caaagaaacc atagaaaact ctataaatgc ttttataaaa
    76561 gaagggtatg atttaaacat taaggactat aaatattcaa tattattggc gaatccaaat
    76621 agtccttata caatattaag tgatggatac tggggtgatg gtgggattcc tggatatata
    76681 tttctatcat tggttcctaa tgaatatact atcaatagat taccagtatt aatagcacat
    76741 gaatgtaatc acaatattag atttcagttt atagagtgga ataataatat aacattagaa
    76801 gaaatgatga taaatgaagg tcttgcagaa aattttgcaa catggatgtt tggagaggaa
    76861 atgttaggac cttgggtcag tagaacagat atcgaaacat taaatactta tataaagcca
    76921 ataataaaaa gtgctttaaa agaaactgga tttcaaaata taacatctta tctttatggt
    76981 gatgatatag ctaaaatgca aggatatttt ccagtagggt tgccttattg tgcaggatat
    77041 gcttgtggat attatatgat taagtattat ttagaaaaga caaataaatc aataatcgaa
    77101 gcgactttat tgccttatag tgagataatc gaagcagtaa aagagttttg ggaataaatt
    77161 ttataaattg cataatttga aataaattat gcaattattt tatttttaag aaaaaataaa
    77221 ttgacaataa tttataagaa atatacaata atatatataa agaaatattt caatatttaa
    77281 aagttttaag atttcatatc tagagtagct aacattacat gttagtttct tgttacttat
    77341 ctaattactt tatatttcgt agttatatat aaaattacta taaaaaatga aatatattaa
    77401 acaatcaact gaaaaatgaa atgttaataa aaaaaaattc aaattataaa tattaaatga
    77461 atataattaa aacaaatata taaatttaag tataacactc acattttaat gtattgtgaa
    77521 atttttgttg aactttatgt accataactt ataaaattta tgtcatgtta tatttgaata
    77581 agtaataaaa ttgtagatgt aaattaatct acaaaattta tatgaaatgg agttgatgct
    77641 atgacagaat taatgattat aagtgggtta gtactattga tttgtataac ttctagcaag
    77701 gtattgtata aatttggagt accaatatta ttgattttta tcttactagg gatgttattt
    77761 gggtctgatg ggatggtagg aatctatttt gataactatg aactgacaaa acaattatgt
    77821 tctattggac taatatttat aatgtttttt ggagggtttg gaacaaactg gaatatggct
    77881 aaacctgttg ctataccttc tatattaatg tcttcactgg gtgtaataat tacggctggg
    77941 gttacaggta tgttttgcca catggtaata ggaactagtt tacttgaagg tttattaatt
    78001 ggggcaattg cagcttcaac agatgctgca tctgtgtttg ctgtgttgcg ttctcaaaaa
    78061 ctaaacttaa agggttcact tgcatcatta ttagaagttg aaagtggaag taatgaccca
    78121 atagcgtata tgctgacact aattcttttg acattgatga ataattctgg ggtagaactt
    78181 attgcaccaa tggtaataaa acaaatagct tttgggttag ctataggatt tatactagca
    78241 aaattatctg tttatatttt aagacattca aacttcgaga tagaaggatt ttatactata
    78301 tttataacag ctattgcaat actttcttat gcatttagcg agtatcttgg aggaaatgga
    78361 tatttaagtg tttacatttc aggtattatt ataggaaata gtaggatacc tcttaaaaaa
    78421 agtttggttc atttctttga tggaatttca tggattatgc aaataatgtt attctttatg
    78481 ttaggattgt tatctttccc ttctaagtta ccaaatgtat ttggtatagc aatggccata
    78541 tctatattca tgatagttat tgcaagacca ttagcaacat ttatagttct agcaaaattt
    78601 aaattctcaa ataaagaaaa attattcata tcgtgggtag gtcttagagg tgcggcttca
    78661 gtagtttttg caatttatgc tgttactcaa ggagtagcca tagagaatga catattccat
    78721 ataatatttt ttatagcgtt gctttcagta ggaatacaag gttcattgat accaactgta
    78781 gccagaaagt tggatttagt tgatgataat acacctgtat taaaaacatt taatgattat
    78841 gatggagaaa ttagctctaa acttatcgaa gtaaatgttg atgagggtaa taaatgggct
    78901 aataagagta taatagattc taatatacca gacgatatat taatagttat gataaaaaga
    78961 aaggaacagg ttttaatacc taaaggttca accattataa aaacagggga tattttggta
    79021 ttgagtggag atagaattga agaattaata caattataat ttattttgta ttgactttaa
    79081 cctaagggga aagtttaaac ttaaattaat caaatctaat gcgcaggtga tgagatgttt
    79141 tctataggaa tgttttctaa aataaataaa ataactacaa agacattaag gtattatgaa
    79201 gatatagggt tgttaaaacc agaatatgta gatgaattta caaaatatag gtattataca
    79261 acagagcaat tacctaaatt acatcagata ataactttaa aacaaatggg tttatcatta
    79321 gctgagataa aaaaagctgt agaaaatcct gaagaagttg aaaatatttt aatgaataaa
    79381 gaaaaagaaa tgttggatgt aattaaaagt gaagaatata aaataattaa actacgtaat
    79441 taccttatga atttgaaagg agaagtcaat atgaatgatg taattattaa gtctttacca
    79501 aaagttaaag ttgcttctat gagaaaagtg ttaaaggggt atgatgaact ttttaatcta
    79561 tttccaaatg taatggctga agaaatgatg aaaacagggt gtacatgtgc agttcctgat
    79621 tattgtttta atatatatca tgatggtgaa tatagagaga ctgatatgga tgttgaaatg
    79681 tgtcaagcta taaatgattt aaaggaagat acggatatat taaaatttaa agaattagat
    79741 gcagttgaaa atgcagtttg tatattacac aatggtccat attcaacatt aggaaaaact
    79801 tacgcagatg catttaaatg gatagaggat aataaatata aagtggttgg aaatcctaga
    79861 gaatcttata tagatggtat atggaataaa gagagtgaag aagactggtt gacagaagtt
    79921 cagataccag tgaaacgcat gttataatat gtctacaaag tataaataag aataaaaaag
    79981 gtggtgtctc aaagtaaaca ttttagttta tgagacactc tttttcatat gaaatcaagt
    80041 atatttttat aattacgatt atgaaaaaag gcttatctat atttttagac agcctcttat
    80101 ttttttatta taatagtttt ctaaagatgc tctatttata aatttttatt aaagacttta
    80161 taaataattg tat
//
LOCUS       DAYASV010000004        67320 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYASV010000004 DAYASV010000000
VERSION     DAYASV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.004"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            203..319
                     /locus_tag="ACGI68_000282"
     CDS             203..319
                     /locus_tag="ACGI68_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein"
                     /protein_id="HFH0136740.1"
                     /translation="MDDDINMVAIRPEDIRVEFNRDFKEIIDLKLRPYRYKN"
     gene            483..1550
                     /locus_tag="ACGI68_000283"
     CDS             483..1550
                     /locus_tag="ACGI68_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0136741.1"
                     /translation="MKFKKLVSLGILSTLLLSVITGCSNVSSGSNLKSNNKTKGELTV
                     YTAIEEDSIDTYLATFKEKYPDIKLNVLRLSVGGITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNSAFKDDKKVPSWVGIDAWMTGITVNTKELKAKNISA
                     PSSYEDLIKPEYKDLISMPSPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            1742..2272
                     /gene="rnmV"
                     /locus_tag="ACGI68_000284"
     CDS             1742..2272
                     /gene="rnmV"
                     /locus_tag="ACGI68_000284"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFH0136742.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            2443..3312
                     /gene="rsmA"
                     /locus_tag="ACGI68_000285"
     CDS             2443..3312
                     /gene="rsmA"
                     /locus_tag="ACGI68_000285"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFH0136743.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            3378..4640
                     /locus_tag="ACGI68_000286"
     CDS             3378..4640
                     /locus_tag="ACGI68_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136744.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEENEESDRDEDSEE
                     EYIIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSDDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            4791..5906
                     /locus_tag="ACGI68_000287"
     CDS             4791..5906
                     /locus_tag="ACGI68_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0136745.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            6092..6988
                     /locus_tag="ACGI68_000288"
     CDS             6092..6988
                     /locus_tag="ACGI68_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0136746.1"
                     /translation="METRYEEANKITIQSILWNIVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            7058..7855
                     /locus_tag="ACGI68_000289"
     CDS             7058..7855
                     /locus_tag="ACGI68_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136747.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(7910..9262)
                     /gene="murC"
                     /locus_tag="ACGI68_000290"
     CDS             complement(7910..9262)
                     /gene="murC"
                     /locus_tag="ACGI68_000290"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFH0136748.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            9481..10359
                     /gene="purR"
                     /locus_tag="ACGI68_000291"
     CDS             9481..10359
                     /gene="purR"
                     /locus_tag="ACGI68_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFH0136749.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            10515..10793
                     /gene="spoVG"
                     /locus_tag="ACGI68_000292"
     CDS             10515..10793
                     /gene="spoVG"
                     /locus_tag="ACGI68_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HFH0136750.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            11006..12385
                     /gene="glmU"
                     /locus_tag="ACGI68_000293"
     CDS             11006..12385
                     /gene="glmU"
                     /locus_tag="ACGI68_000293"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFH0136751.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            12480..13430
                     /locus_tag="ACGI68_000294"
     CDS             12480..13430
                     /locus_tag="ACGI68_000294"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFH0136752.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      14268..14353
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            14515..15036
                     /locus_tag="ACGI68_000295"
     CDS             14515..15036
                     /locus_tag="ACGI68_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0136753.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTPDGQTGLSVLETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTNTEGVTLTEAQSAKLLSDIGENKIYTSATTNSLGNPLTSNTKIDNNVLY
                     IVLIDNTVMDTTK"
     gene            15187..16863
                     /locus_tag="ACGI68_000296"
     CDS             15187..16863
                     /locus_tag="ACGI68_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFH0136754.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            16875..18083
                     /locus_tag="ACGI68_000297"
     CDS             16875..18083
                     /locus_tag="ACGI68_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFH0136755.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            18098..19810
                     /locus_tag="ACGI68_000298"
     CDS             18098..19810
                     /locus_tag="ACGI68_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFH0136756.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            19803..20711
                     /gene="pilO"
                     /locus_tag="ACGI68_000299"
     CDS             19803..20711
                     /gene="pilO"
                     /locus_tag="ACGI68_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFH0136757.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            20738..21304
                     /locus_tag="ACGI68_000300"
     CDS             20738..21304
                     /locus_tag="ACGI68_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0136758.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            21341..21868
                     /locus_tag="ACGI68_000301"
     CDS             21341..21868
                     /locus_tag="ACGI68_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0136759.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            21889..23427
                     /locus_tag="ACGI68_000302"
     CDS             21889..23427
                     /locus_tag="ACGI68_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136760.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRDISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRDSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYIWNSKWDTLEKLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            23527..24609
                     /locus_tag="ACGI68_000303"
     CDS             23527..24609
                     /locus_tag="ACGI68_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFH0136761.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            24590..25372
                     /locus_tag="ACGI68_000304"
     CDS             24590..25372
                     /locus_tag="ACGI68_000304"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFH0136762.1"
                     /translation="MDIIIVNLYVFMVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILCGKQLLELYFSMVI"
     gene            25654..26124
                     /locus_tag="ACGI68_000305"
     CDS             25654..26124
                     /locus_tag="ACGI68_000305"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFH0136763.1"
                     /translation="MVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFFNIVQ
                     FDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFILA
                     FLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            26138..26698
                     /gene="pth"
                     /locus_tag="ACGI68_000306"
     CDS             26138..26698
                     /gene="pth"
                     /locus_tag="ACGI68_000306"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFH0136764.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            26715..30101
                     /gene="mfd"
                     /locus_tag="ACGI68_000307"
     CDS             26715..30101
                     /gene="mfd"
                     /locus_tag="ACGI68_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFH0136765.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            30132..31127
                     /locus_tag="ACGI68_000308"
     CDS             30132..31127
                     /locus_tag="ACGI68_000308"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0136766.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            31251..31790
                     /gene="spoVT"
                     /locus_tag="ACGI68_000309"
     CDS             31251..31790
                     /gene="spoVT"
                     /locus_tag="ACGI68_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HFH0136767.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            31910..33523
                     /locus_tag="ACGI68_000310"
     CDS             31910..33523
                     /locus_tag="ACGI68_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFH0136768.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            33630..35078
                     /gene="mazG"
                     /locus_tag="ACGI68_000311"
     CDS             33630..35078
                     /gene="mazG"
                     /locus_tag="ACGI68_000311"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFH0136769.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            35167..35445
                     /locus_tag="ACGI68_000312"
     CDS             35167..35445
                     /locus_tag="ACGI68_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFH0136770.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            35517..35759
                     /locus_tag="ACGI68_000313"
     CDS             35517..35759
                     /locus_tag="ACGI68_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFH0136771.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            35821..36075
                     /locus_tag="ACGI68_000314"
     CDS             35821..36075
                     /locus_tag="ACGI68_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFH0136772.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            36076..36609
                     /gene="yabQ"
                     /locus_tag="ACGI68_000315"
     CDS             36076..36609
                     /gene="yabQ"
                     /locus_tag="ACGI68_000315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HFH0136773.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            36615..36899
                     /locus_tag="ACGI68_000316"
     CDS             36615..36899
                     /locus_tag="ACGI68_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFH0136774.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            37012..37932
                     /locus_tag="ACGI68_000317"
     CDS             37012..37932
                     /locus_tag="ACGI68_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HFH0136775.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIIFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYGEIDKTLNHIKDKGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            38022..40394
                     /gene="spoIIE"
                     /locus_tag="ACGI68_000318"
     CDS             38022..40394
                     /gene="spoIIE"
                     /locus_tag="ACGI68_000318"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HFH0136776.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            40745..42637
                     /gene="pepF"
                     /locus_tag="ACGI68_000319"
     CDS             40745..42637
                     /gene="pepF"
                     /locus_tag="ACGI68_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFH0136777.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            42751..43716
                     /gene="galU"
                     /locus_tag="ACGI68_000320"
     CDS             42751..43716
                     /gene="galU"
                     /locus_tag="ACGI68_000320"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFH0136778.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            44216..45820
                     /gene="rho"
                     /locus_tag="ACGI68_000321"
     CDS             44216..45820
                     /gene="rho"
                     /locus_tag="ACGI68_000321"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFH0136779.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            45973..46173
                     /gene="rpmE"
                     /locus_tag="ACGI68_000322"
     CDS             45973..46173
                     /gene="rpmE"
                     /locus_tag="ACGI68_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFH0136780.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            46318..47211
                     /locus_tag="ACGI68_000323"
     CDS             46318..47211
                     /locus_tag="ACGI68_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HFH0136781.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            47229..48077
                     /gene="prmC"
                     /locus_tag="ACGI68_000324"
     CDS             47229..48077
                     /gene="prmC"
                     /locus_tag="ACGI68_000324"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFH0136782.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            48121..49185
                     /gene="prfA"
                     /locus_tag="ACGI68_000325"
     CDS             48121..49185
                     /gene="prfA"
                     /locus_tag="ACGI68_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFH0136783.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            49314..49994
                     /locus_tag="ACGI68_000326"
     CDS             49314..49994
                     /locus_tag="ACGI68_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFH0136784.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            50023..51063
                     /locus_tag="ACGI68_000327"
     CDS             50023..51063
                     /locus_tag="ACGI68_000327"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFH0136785.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            51080..51529
                     /locus_tag="ACGI68_000328"
     CDS             51080..51529
                     /locus_tag="ACGI68_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HFH0136786.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            51546..51998
                     /gene="rpiB"
                     /locus_tag="ACGI68_000329"
     CDS             51546..51998
                     /gene="rpiB"
                     /locus_tag="ACGI68_000329"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFH0136787.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            52034..52663
                     /gene="upp"
                     /locus_tag="ACGI68_000330"
     CDS             52034..52663
                     /gene="upp"
                     /locus_tag="ACGI68_000330"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFH0136788.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            52958..53773
                     /gene="yqeB"
                     /locus_tag="ACGI68_000331"
     CDS             52958..53773
                     /gene="yqeB"
                     /locus_tag="ACGI68_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFH0136789.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            53999..54436
                     /locus_tag="ACGI68_000332"
     CDS             53999..54436
                     /locus_tag="ACGI68_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFH0136790.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            54621..54848
                     /locus_tag="ACGI68_000333"
     CDS             54621..54848
                     /locus_tag="ACGI68_000333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HFH0136791.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            54853..55239
                     /locus_tag="ACGI68_000334"
     CDS             54853..55239
                     /locus_tag="ACGI68_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFH0136792.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            55242..55946
                     /gene="atpB"
                     /locus_tag="ACGI68_000335"
     CDS             55242..55946
                     /gene="atpB"
                     /locus_tag="ACGI68_000335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFH0136793.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            56015..56275
                     /gene="atpE"
                     /locus_tag="ACGI68_000336"
     CDS             56015..56275
                     /gene="atpE"
                     /locus_tag="ACGI68_000336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFH0136794.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            56398..56895
                     /gene="atpF"
                     /locus_tag="ACGI68_000337"
     CDS             56398..56895
                     /gene="atpF"
                     /locus_tag="ACGI68_000337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFH0136795.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            56892..57437
                     /locus_tag="ACGI68_000338"
     CDS             56892..57437
                     /locus_tag="ACGI68_000338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFH0136796.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            57454..58956
                     /gene="atpA"
                     /locus_tag="ACGI68_000339"
     CDS             57454..58956
                     /gene="atpA"
                     /locus_tag="ACGI68_000339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFH0136797.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            58984..59862
                     /gene="atpG"
                     /locus_tag="ACGI68_000340"
     CDS             58984..59862
                     /gene="atpG"
                     /locus_tag="ACGI68_000340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HFH0136798.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            59875..61269
                     /gene="atpD"
                     /locus_tag="ACGI68_000341"
     CDS             59875..61269
                     /gene="atpD"
                     /locus_tag="ACGI68_000341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFH0136799.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            61272..61532
                     /gene="atpC"
                     /locus_tag="ACGI68_000342"
     CDS             61272..61532
                     /gene="atpC"
                     /locus_tag="ACGI68_000342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HFH0136800.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            61994..62374
                     /gene="acpS"
                     /locus_tag="ACGI68_000343"
     CDS             61994..62374
                     /gene="acpS"
                     /locus_tag="ACGI68_000343"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFH0136801.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRLFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            62389..62850
                     /locus_tag="ACGI68_000344"
     CDS             62389..62850
                     /locus_tag="ACGI68_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFH0136802.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            62871..63461
                     /gene="gerS"
                     /locus_tag="ACGI68_000345"
     CDS             62871..63461
                     /gene="gerS"
                     /locus_tag="ACGI68_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="germination lipoprotein GerS"
                     /protein_id="HFH0136803.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQDDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            63476..64633
                     /gene="alr"
                     /locus_tag="ACGI68_000346"
     CDS             63476..64633
                     /gene="alr"
                     /locus_tag="ACGI68_000346"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFH0136804.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEKSVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            64666..64953
                     /locus_tag="ACGI68_000347"
     CDS             64666..64953
                     /locus_tag="ACGI68_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HFH0136805.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            64922..65305
                     /locus_tag="ACGI68_000348"
     CDS             64922..65305
                     /locus_tag="ACGI68_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFH0136806.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            65476..66303
                     /locus_tag="ACGI68_000349"
     CDS             65476..66303
                     /locus_tag="ACGI68_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0136807.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            66296..67237
                     /locus_tag="ACGI68_000350"
     CDS             66296..67237
                     /locus_tag="ACGI68_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFH0136808.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEVIIAKRM"
BASE COUNT        27191 a         7147 c        12565 g        20417 t
ORIGIN      
        1 taaaatattg agcgattatt aaaaagatat atatataatt taatttgagc agtttgttat
       61 atattataaa aaaagagaaa aataaaaggt aaattgtttc tggataactt aaaattggaa
      121 gtaagaatat ttaactagag aagaattatt taagtttagg ttagctctag gagtagctga
      181 ttttatagga acaatagatt ttatggatga tgatataaac atggtagcaa ttagaccaga
      241 agatataagg gtagaattta atagagattt caaagaaatt atagacctca aactcagacc
      301 gtatcgatat aaaaactaaa gttagatgat atagtgttgt agataggacc atcagctgtt
      361 atggcagtat tagtgctttt aacaaatata atagtaaaac ttttgtatga attgataata
      421 aaaaaattaa aaaccaagac ataaaaatgg caacaaaaag aagttgaata agggaggtaa
      481 aaatgaaatt taagaagtta gtctctttgg gaatattatc aacactttta ttgagtgtaa
      541 taactggatg tagtaatgtt tcatcaggtt caaacttaaa gtctaataat aagacaaaag
      601 gagagttaac agtttataca gcaattgagg aagatagtat agatacatac ttagctacat
      661 ttaaagagaa atatccagat attaaattga atgtacttag attatcagta ggaggtataa
      721 cagcaagatt attagcagaa aaagataatc ctaaagcaga tgtgttatgg ggtgttgcag
      781 ctacaagttt gataatttta gatgacaaag aaatgctaga gccatacgct ccgaaaggct
      841 ttgataaggt aaattctgca tttaaagatg ataagaaagt tccttcttgg gttggaatag
      901 atgcttggat gactggtata acagtaaata ctaaagaatt aaaagctaaa aatatatcag
      961 ctcctagttc ttatgaagat ttaataaagc cagaatataa agatcttata tctatgccta
     1021 gtccagcttc ttcaggaaca ggatatttaa cagtatcaga actcatacaa attatgggtg
     1081 aagaaaaagc atggcagtat atggataaat tgcataaaaa tattggtgta tacactcagt
     1141 ctggttcagc tccagctata ttagcagctt caggagaata tcccattggt atatcttttg
     1201 gaaatagagg tataaagtta aaaggtgaag gttatccagt agaagtagta tttccaaaag
     1261 aaggttctgg atgggagata gaatcaaatg cacttgtgaa aaaagataat ataaaagaag
     1321 catcaaaagt atttttagat tgggctataa gtaaagatgc aatgaatgag tattctaaga
     1381 attttgcagt tactacaatt gacacgggaa atccggtgcc agaaggattt ccaaagaatc
     1441 cattagaaca gatgatagat aatgatttaa aatcagcagc aagaaataga gaggctatac
     1501 taaataaatg gatagaaaaa tatgatggaa agacagaaaa agagagctaa gccttgcaat
     1561 aaaatttagt acaaaaagcg cctagaatat aagttttata tctaggctct tttgatatat
     1621 ttatttaaaa tgcatatttc tatataaaaa taaatagttt agtaccttga ttaaattaaa
     1681 taaaaacctt aaaataaaga gatgtaaaga aaaactaaaa acgaataaaa ggtgaattta
     1741 tatgataaaa gaaataatag tagtagaggg tagagatgac gttacagcag ttaagagagc
     1801 aatagatgca gaacttataa ctacaggtgg atttggattt ccaaaaggtg taatggagcg
     1861 tataaaagct gctaatgaaa gacgtggtgt tataatattt acagacccag attttgcagg
     1921 tgaaaaaata agaaaaaaaa tagcatcaga agttccagga tgtaaacatg catttctacc
     1981 gagagaggaa gctaaaaagg atggagatat aggtattgag aatgctactc ctcaaagtat
     2041 aatagcagct ctgaataaag ttagaactga aaatacagaa aaaagagatg aatttaagca
     2101 agttgactta attagaaatg gtcttatagg taatgaagaa gcatcactta gaagagatgc
     2161 acttggaaaa atacttggaa ttggatatgg aaatgcaaaa caatttttaa atagattgaa
     2221 taattatgga gttgaaagag aagaatttat agatgcatta aaacaaatct aaacgtaatg
     2281 tttaatgtat gaaaaatatg tatataaaat aaattgtgga attacggtta atttaagatg
     2341 aattatttat aatatacacc ttattttaaa tcaatgtaaa tacatataaa taagtataat
     2401 taataaaatt aaatatcaaa ataagttaaa aaggagtaaa taatggatag actatcttca
     2461 cataatgcaa caaaggaagt agtacaaaaa tataatttta aattttcaaa gtctttaggt
     2521 caaaatttct tgatagatag taatataatt gataaaatct tatctggagc tagaatcaca
     2581 agaggagata atatcataga agtaggacct ggtataggaa ctcttactcg tgagatgggt
     2641 aaaatagcag aaaaagtagt ggctatagaa atcgatagaa atttaattcc tatattgaaa
     2701 gataccttgt cagatttaga taatacagag gttgtaaatc aagatatact aaaagtagac
     2761 atacaggaat tagtaaaaga taaattaaat ggtgggccag ttaagcttgt agcaaatctt
     2821 ccatattata taactactcc aatagttatg aaatttttag aggaagacat acctgtaaca
     2881 gacatagtag tcatggttca aaaagaagtt gcagatagaa tgaatgctat tccgggtact
     2941 aaagattatg gggcattatc tatagctgtt caatactatt gtgatacaga gattgtggca
     3001 aaagctccaa gacatatgtt tataccacaa ccaaatgtag attccacagt tataggtcta
     3061 catgtaagag ataaaagaaa atatgatgtt cataatgaag acatattttt taaaacagta
     3121 aaagcatcct ttggacaaag aagaaaaact cttcttaatt cgttgggagg tcttggtttt
     3181 cttaataaag atgaaataag agaagtatta aaagaagcta atatagacga aaaaagaagg
     3241 ggtgaaactc ttagtataga agagttttca gttctttcaa atataataaa tacaaaagta
     3301 tcttctaaat agaaggtact ttttttaatt ttgttaacat atattgttta gagaaactgt
     3361 gttaaggggg gattgcagtg ggttctaaaa gaaagtttaa aagagactat ataatactag
     3421 aagctaaaga cataaacttt agatacaaag aacgagtact gccaaaagct tttgcaaaag
     3481 ttgaagtaaa tgatgaaaaa gcaattattg cattatatgt agaaaatcta aaatatgtta
     3541 aagatgggta taaagcagta gcaataaaag atgaccatgg attaatagac ttaggaaata
     3601 taattttaaa tgaacaagga agaggtgaat ttgttttaga tttagaagat tatgatgatg
     3661 agataaaagg tattgcatta ttgtatgaaa gaaatatacc tttaattgga tttaaaggaa
     3721 ataagcttga aaattatgag gacatattat ttcaagaggt agaggaagaa gaatatgaag
     3781 aatatgaaga gttggaagaa gaagacgaag agatagagga gaatgaggaa agtgatagag
     3841 acgaagatag tgaagaagaa tatattatag aagaatatga tgaagatgag tatgaagact
     3901 attgggaaga tgatgaagat attgaagaag gagagttaaa agcaagaacg gaagtagaag
     3961 attctgatga tgaggaaata gaagagttca ataattataa ggaaatagaa aattataata
     4021 gggttaaagt acaggaagaa tatatagaca tagaatatga tgaagaagaa gaggaagaag
     4081 aagtttttga agtaaataga gaagaagata ggtttgtttc ctcaaaacct actaaatcaa
     4141 agaaagttca aaaagatcag aataatattg aaaaaataaa aagagctaat ggagtaagtc
     4201 aaggagagag tggaaataaa caaaccagta cattacttat gccaagacaa ataaaaaaag
     4261 gtttgaaatt atttaaagaa gtaaaacctt ttgtaacaga ttatatagat aagacaagat
     4321 ggtggaaaat agagataaat cctacaacac tgtgtggata tacaatgcct tatttaggat
     4381 atttaaacgt attgaattac actatgtata gtgatgcagt tttacattca tataaataca
     4441 gacactattt atttggagtt cagtatgatg aatataataa gagaaaacat tatatttatg
     4501 ctatcccagg aaatagaaat gaacagccag acaatggaag cacaggattt ttaagatatc
     4561 aagcttgtga taatagaaca aatagtttag gatattggct atgttttata gattctagac
     4621 aaagaaatat agttaggtaa aaataataaa aatgaaaaaa tttagactca attcttattt
     4681 tactgaaaaa attaatgaaa tgtagtacaa aactttaaaa actattgata atatttaaat
     4741 attacactac aatatatctt gaaataccta ttatattatg gaggaaaatt atgataaacg
     4801 agcaaagaat tttaaatgag tttcttgaat tagtacagat agatagttta tctttaaaag
     4861 aggggaatgt tgctaaagta ttagttaaaa aattagaaga aataggttgt tcagtagtaa
     4921 tagacaatgc tggtgaaaaa gcaaatggag aaactggtaa tataatagct acattaaaag
     4981 gaaataaaga aggaaagaaa atattattta gttcacatat ggatacagta actccaggta
     5041 taggggtaaa acctatagtg gatgaagcaa atggaataat aaaaagtgat ggtactacaa
     5101 tattaggttc tgatgataaa gcaggtatag cagctatatt agaaggctta agatatataa
     5161 atgaaaataa catagaacat actgatatac aagttgtatt ctctatatgt gaggaatgtg
     5221 gattagttgg agctaaaaat ttagactatg gaaaaataga ttctgaatat gcatttatat
     5281 tagatagtgg tggttctcca ggtgaaataa tagtaaaagc tcctgcacaa gatgtaataa
     5341 atgttaagat attaggaaag actgctcatg caggtcttga acctgaagct ggaataagtg
     5401 ctataatggt agctgccaga gcaatagaaa atatgaattt acttagaata gacgaagaaa
     5461 caactgctaa cataggaatt ataaacggtg gaacagctac aaatatagta acaggagaag
     5521 ttaatatagt tgcagaagcc agaagtctaa aagaaaataa attagatgtt caaactaaac
     5581 acatggtaga gacttttgaa aaagctgcga aagattttgg agctcaaatt gagatagatg
     5641 ttaatagagc atacacacca atagatgtta gtgaggattc tgaaataata aaacttgcaa
     5701 agaaggcttt ttcaaactta ggtatagaag gtcatactga atctacagga ggaggtagtg
     5761 atacaaatat attaagtaag aatggtatcg aagctataac tttaggaatt ggtatgaaaa
     5821 atgctcatac actaagtgaa catatagcta taaaagactt atatgattct gctaaaatgg
     5881 tagtggagat aataaaggaa gcataaattt aaataatagt gtataatata tatatcgcct
     5941 agcttaagct aggtgatttt tcattttata gaagattgat aatctctatt gaaagcaaag
     6001 agtatacatt tgcatataag agagtgttta atttatgttc aaatgtaggt tcttaataat
     6061 atttagattg tttcataagg gaggaataaa tatggaaaca aggtatgaag aagcaaataa
     6121 aataacaata caatcaatac tttggaatat agtattaaca ataattaagg ttatagctgg
     6181 tgttattggt aattctagcg ctatgattgc ggatggatta cattcagcat cagatattat
     6241 aagttctata ggagtactaa taggcaatta tgtatcttct agacctggag atagagagca
     6301 taattatggt catgagaaag cagagacatt agtatcattt gtgttatcaa tattgcttat
     6361 tttcgtttct ataacaatag gtatagaggc aattaaatct ttatttaact tagatgcatt
     6421 aagtgttcca tcaatattac cattagtagt atcagttatt tcaatcctga taaaagagta
     6481 tcaatataga ataacaataa aagttgctaa aaagataaat tcaccagccc taaaagctga
     6541 tgcttggcat cacaggtcag atgctttatc ttcagtagct gcttttatag gtataggtgg
     6601 ttctatttta gggtttaaac cattagaccc aattgcgtca gttgtagtgg ctatatttgt
     6661 agctaaagta ggaattagta tacttataag ttctgttaat gaattgatgg atgtttcagt
     6721 tgatgaagaa gagataaaag aattaaaatt tattgtagca gatacagaag gtgttaaaaa
     6781 tcttggtgat ataaagacta gaaaacatgg agctatggca tatgtagatt tgactatatg
     6841 tgtggatgaa aatttgacag ttaagcaagg tcatgatatt gctaccaagc ttgaaaaaca
     6901 tataataaaa catatggaat ttgtaaaagg tataacagtt cacgttgaac catgtactaa
     6961 ttgccaagga aataagtgta ataattaatt aaaacttaaa aaaaagtgta aaaagttata
     7021 taattgtttt atgattataa actctagggg tggttaaatg agcaagaaaa gaaaactaaa
     7081 gaaaaaagct gcaatattaa taggagtagt tgttttcttt ataatatact ttatatcctt
     7141 tagaatagga ataaatatgg aaaaggataa aagtgaagtg ccaaagaagg tagaaaaaga
     7201 agaagaagat aataggtctt taaggactca aataaaagag aagaagaagg tttatatatc
     7261 agataaaaat atagaaaaca taaaaattga agaagatatg tgggatgaaa taaagttatt
     7321 tttctcagat tttaagaaag taagaaatac tacttcttct tacgaagcag tttatgaagg
     7381 ttattctgat gatggagtta aattttctac tgaccttaac ttttttagag tatatactgt
     7441 aaataaagaa gaatattata aagtaccagt tagttctaag gatgctttta agaaactttt
     7501 agatgagagt atatatactt cgtttgacct tttaagtcat tataaagatt ggaaagaggt
     7561 tactataact catggaaaag aaattaaaaa agttcataga tggaaatttg atgatttagc
     7621 acataagatg atttcaaaga gggttgtagg aaaagttcaa cctgagaaaa gtaaggaaag
     7681 aagtgactat aattttacta taaatataaa aggtgataat tttgatgtaa aagttgatac
     7741 aatgggaaaa gattatgtaa aagttacttc taagaaaaat gtatcttact atgaggttca
     7801 tacaagttta tttgattacc ttaaaaatga gatatttgaa attggaaaga aataagactt
     7861 taagaaggct atcaaattag atatttgata gcctaaatta ttttatatat tatttttcaa
     7921 ccaacaagtc tgcaacctta taaattggac cagcaccagc agttattact aaatcattat
     7981 ccttaacatt ttcacgtaag tattttacta tatcttcaaa ttcttttata tagatggcat
     8041 ctacattgtt ttgatataat ttctcaacca agtccttaga atgtatatct cctgggtctt
     8101 tttccctagc agcatatata tctgttatga taactttatc agcagcatag aatgcctctg
     8161 aaaattcacc taaaagagat tttgttctag tataagtatg tggctggaag atacaccata
     8221 aagttgattt ttttaatttt ttggcagcag ctaaagttgc ttttaactca gttggatgat
     8281 gtgcataatc atctactact aaagcattct tatagtaccc tttagtttca aatcttcttc
     8341 cgacaccttt ataaattttt atattttttc ttatagtctc taagtcaata ccagaaacaa
     8401 gagctgccat tatggcagaa gaagcattgt atatgttatg aagaccatat acagaaagtt
     8461 caaattctcc taaatctctt ccttcatatt ctattctaaa tattccatga ccactattgt
     8521 caaagtgaat atcttttatt acagcatcat tgtcagaatc tctgccgtat tttataatag
     8581 tagctttaac atcatacaat atgtcatcaa tattttcatc atcgccattt attatgaaat
     8641 agccttctgg gggtaataat tttccaaatt tgttaaaaga agccttgatt tcatctattc
     8701 cagagaaata atcaagatgg tcttcttcaa catttaaaac tatagatatc tttgggttaa
     8761 aattaaggaa actatctaca tattcacatg cttcagttat aaagtggttg gaattaccta
     8821 tcttaacatt accacctatc atacttaagt taccaccaac tagtatagtt gggtctaagt
     8881 ctgcatattc aaagattgta gataacatag atgttgtaga agtttttcca tgtgttccag
     8941 aaactgctat agaattttta tattccctca tgatttggcc taaaaaagtt gctctgttca
     9001 ttaccaattt atttttttct ttagcagcca caagttcttc attgtctgga tgaactgctg
     9061 ctgtataaac aaccatattt acatcatctg atatattatc ttttttctga ccaatataaa
     9121 tagttgcccc ttggtcccta agtttgtcta ataggtatga ttcgtttgaa tccgaacctg
     9181 aaacttgata acctttattg atacatattt cagctaatgc actcatgctg ataccgccaa
     9241 ttcctataaa gtgtatattc ataataaaac cacctttctt tttaattaag tcctacaata
     9301 tgaactattt ctgtggtata ataatataat gttcaatatt ttataattaa tatgcatata
     9361 atattatatg tattttattt ttgaatataa taaaacataa gtaatattat accacaataa
     9421 gaattgttat gcattagtta gtttaacgtt atataaaggt acttgtagga ggaaataata
     9481 atgaaattta aaagaacaga gagaattgga gcaatagtta aaatactgtc tgataatcca
     9541 aataaaatat atactttaag ttattttaca aatcaattta atgcagcaaa atcaacaata
     9601 agtgaagatt tattagttgt aaaaaatgta tttgagaagc tacatttagg taaagttata
     9661 actatttcag gagcagcagg aggagtaaaa tatataccta aaacttctat agcagaaaat
     9721 gcagagttct taatggaatt atgcgagaga atatgtgaca agtctagaat actatctggt
     9781 ggttttttat acttgattga cctaatttac gaccctacaa tagctgctaa gataggaaag
     9841 atattcgcat caaatattga gtatgtagat gcagattatg ttgtgacaat ggaaacaaaa
     9901 ggtataccta tggcacttat gactgcaaaa gctatgaatt tacctctagt tataataaga
     9961 aaagatataa aggtatcaga aggtcctaca cttagcatga catatgtgag tggaaacagt
    10021 tctaaagttg aaagtatgag tttgccaaga aaagctctta aaccagatag taaagttata
    10081 attatagatg atttcatgag gggtggaggt actataaaag gaatggtaga ccttatgaat
    10141 gaatttggtg cagaggttat aggtactgga gtattcatat ctacaacgaa tccttcagaa
    10201 aaaatggtaa aagattatat ttcattgatt caattggatg ttaatggaga taaaatagta
    10261 gtagaaccaa acttaaagac ttttaaagat gaatatagaa atgaagattt agacgaagaa
    10321 gaagattcag aatttgaatt tgagatagat gaagattaat aaaaaaaata tttagaaaaa
    10381 tatgtaaaaa taaaaagagg atatccctag ttgttcatag aattatttaa aaaacagttt
    10441 atattaattt attccagcga taataatttt aatataaatt aatttctgtc aaaatcaaag
    10501 ggggatattt tggtatgaag ataactgacg taagagtaag aaaattaaca gaagaaggta
    10561 agatgaaatg tatagtttca ataactttcg ataatttgtt tgtagtgcat gatataaaag
    10621 ttatagaagg ccataatggg ctatttatag caatgcctag cagaaaagta ggcgaaggaa
    10681 atttcagaga tatagctcat ccaataaatg cagaaatgag acaagtttta gaagatgcag
    10741 ttttacaagc gtatcatgaa gcattagttc aatgggaagt agctgctgaa taataataaa
    10801 tatataaaat ggagagagcc cgaacattta ggctcttttt aaatgtagaa tttctattaa
    10861 aatatgtact ttttagtaaa aaagtgctat attattagtg ataatgtata tgttgtatac
    10921 aaattgttag ataaatgtat tataatgtat attgattaag agaataatac gaataaaatt
    10981 aaattagatt gaatggagtg tatcaatgaa ctttaaagct ataatccttg ctgctggcaa
    11041 aggaacaaga atgaagtcta aatacccaaa agttatacat aaagtatgtg gaaaagaaat
    11101 ggttaaccat attatagatg tttcaaaaaa atcaggggta aaagatactg tcgtgatttt
    11161 aggacatgaa gctgatgtag ttaaagaaaa gcttgcagaa gaaattataa tagccatgca
    11221 aacagaacaa cttgggacag gacatgctgt aaagatggct aaagaatata ttaatgatga
    11281 agatacaata gttgtacttt gtggagatac tccacttata aaagaagaga cactaaagag
    11341 actatttgaa tatcatatag aaaataaata tcatgctaca gttcttacaa caagagttgg
    11401 aaatccaaca ggttatggta gaataataag agataagaag ggtgaccttc ttaaaatagt
    11461 ggaacaaaaa gatgcaaatt cagaagaaaa gatgataagt gagataaact ctggaattta
    11521 ttgttttaat ggaaagagtc ttagagaagc actagatttg ctaaataata acaattcaca
    11581 aggtgaatat tatttaactg atactgctaa aataatgaga gataaaggat taaaagttgg
    11641 tgcatttgca ggttcaacta ttgaagaact tatgggcgtt aattcaagag tagaattatc
    11701 aaaggcagaa gaaattatga gacgaagaat aaatgaatct catatggtaa atggagtaac
    11761 tataatagac actaactcta cctatataga atctgatgtt atgataggca atgatactat
    11821 aatatatcca ggtgttatgt tacaaggaaa aacaaggata ggttcagatt gtataattgg
    11881 aatgaattca tctataacaa attcagaaat aggtgatggt acagaaataa aaaattcaac
    11941 tataattgat agtaaggttg gagaaaattc tactgttggg ccatatgcat acttaagacc
    12001 aaagagtgac ttaggaaata atgtaaaaat aggcgatttt gtagaagtaa aaaatgctat
    12061 tatagaagat ggctcaaaag catctcacct ttcttatata ggagatgcac atgtcggtaa
    12121 aaatgtaaat attggatgtg gagtagtatt tgtaaactat gatggtaaaa ataaatttaa
    12181 atcaatagta aaagacaatg cgtttatagg ttcaaattct aatttagttg caccagtagt
    12241 tgtagaagaa aaaggatata tagcaacagg ttcaacaata acacatgatg tcccagatgg
    12301 tgctttggca atagcaagag aaagacaagt tatcaaagaa ggatgggtag aaaaaaagaa
    12361 tcaaaaagac gaccaaagta agtaattatg tagtttatta ttttggctaa aaaatttagc
    12421 tcgaaattat tataaattat attcattagt attttaaaat tttcaggagg gttacataaa
    12481 tgaatactag cggaagcgag attaaaatta ttgcaggtaa ttcatcgaaa gagttagcac
    12541 aaaaaatagc tgactatata ggtgtatcag tactagattg tgaagtaggt acatttagtg
    12601 atggtgaaat atgtgtaaac atgaatgaga cagtaagagg ttgtgatgtg tttgtagtgc
    12661 aatctactaa cagcccagta aatgataact taatggagtt attaatctta attgatgcgt
    12721 taaagagagc atcagcaggt agaataacag cagttattcc ttactatgga tatgcaagac
    12781 aagacagaaa ggctaaggca agagatccaa tcactgctaa attagttgca aatttaataa
    12841 ctgctgctgg agcagataga gtattaacaa tggatttaca tgcagctcaa atacaaggat
    12901 attttgatat accattagac cacttactag gtggaacaat attagcaaat tattttaatg
    12961 aaaagaaaat agaagattta gtagtagtat ctcctgactt aggaagtgtt actagatcaa
    13021 gaaaatttgc taatacttta aatggagaag ttccaatagc tataatagat aaaagaagac
    13081 cgaaagctaa tgtatgtgaa gttatgaact taataggtga tgttaaaggt aaaaatgtta
    13141 tactattaga cgatatgata gatacagcag gaacaatagt taatgctgca aatgcactta
    13201 aagagtttgg agcaaaagat gtttatgctt gttgtactca tggtgtgtta tcaggtccag
    13261 ctattgaaag aattgctaat tcagaaataa gtgagttaat agttcttgat actatacaac
    13321 ttcctgaaga gaaaagaata gataagataa agataaaaac agtagctcct ttatttggag
    13381 atgctataag aatgatattc tctaatgaat cagtaagtaa attattctaa tttattgtat
    13441 ttatgagagt tgtttaaaag gatataaacc ttttgagcaa ctcttttttg attttatact
    13501 tatggaatgt tatttttttc ttgttagaaa ggtatatatt attgtaaaaa gagattatag
    13561 tttaaaatta tgtatattta agtaaaataa acctaataaa gggtatatta ttcaaaatat
    13621 tatgaatata aaaaatatta caaaaaagta ttgaaaaaat tcagattatg tagtattatg
    13681 taaatataaa taaagaaaga ggtgtttcca atgggaaggt tagaagatga acccctaata
    13741 attaaatgat tcagtcacaa tctaaagtga atataaaaat ctattgaaag tttatttata
    13801 agaattgaaa atagtatcta ttctatcttt agtgtttgtg agagggttct ggattatata
    13861 agattactgt aaagagtaat tttgcacaat aagcatatct gtaaatgaag agtttcgttt
    13921 atataatgat tgtggctgaa tcataactat aatagataga atgtttataa tagatgattt
    13981 tataaaatac tatattgtaa tagcttttag ctttcaaata aatattagca aaggatggaa
    14041 atgacaatga taaaactaaa tgataattta taaatatctc cttacgatat ataataattg
    14101 tagggagatg tttctttttt tgtatagcat aatttttcta gaaattctgt ttcaatatgt
    14161 aaaaagttgg tatatataaa tagtataaac agtgttgcta aaatatggta gatattatat
    14221 aatataagtg gatatgtata tacattatga gttatataat ttgaatttaa taaaatagaa
    14281 acgttgattt atgttctgta atgtgggcac cttggagcat attgagttag tggtgcaacc
    14341 ggctatgaaa ttgtatttat ttataaatac tattattttc atagcttttt ttatttaaaa
    14401 aatggttata tgttagaaat aaatttggtt agtacgttta aataatttta cttagatttt
    14461 taaccagata tattgtaagt aagtattaat taaaaaattt aagggggaat aaaaatgaag
    14521 ttaaaaaaga ataaaaaagg tttcacttta gtggaattat tggtagtaat tgcaattata
    14581 ggtatattag cagtagtggc agttccagct ttatttagta atataaacaa ggctaaggta
    14641 gcaagtgttg agtctgatta tagttcagtt aagagtgcag cattatctta ttattcagat
    14701 actaataaga taccagttac accagatggt caaactggtt taagtgtttt agagacttat
    14761 atggagtctc tgcctgataa agctgatata ggtggaaaat ataaattgat taaagttggt
    14821 aataaattgg tattacagat aggtacaaat actgaaggag ttacattaac agaggcacaa
    14881 tcagcaaagt tattgagtga tataggtgaa aataaaatat atacaagcgc tacaacaaat
    14941 agtttgggaa atccattaac aagtaataca aaaatagata ataacgttct atatatagta
    15001 cttatagata atactgtgat ggacacaaca aaatagttaa aacaatattt ttataaaaat
    15061 attgtaaatt taatgaaaaa tataatataa aagacaggaa gaaaatcttc ctgttttttt
    15121 atactatttt ctttaatgtt caattatgaa tgatgatata attgagatag taaaggagtg
    15181 aaacctgtgg ctaaaaaagt tagaatagga gacaaactgg tcgaaaaagg atatataaca
    15241 gaagaacagc ttaaatgggc actatcagaa caaaaaaaca gtggaaaaag actaggagaa
    15301 tttctagtac aagaagggct tatagatagt aacttactaa taagtgtatt aaaagaactc
    15361 ttggatatag aaagtatatt tttagaaggg acagaaatag acactttagc tacaaaaatg
    15421 gttccagaaa atatatgcaa aagatatact gtatttccat ttaaaattga tggaaataaa
    15481 atatgtctag ccatgtcaga cccacaagat agagaagcag ttcaagatgt aaggcgtatg
    15541 tctggtaaag acgtagagat atttatatca agtacagaag atataaacaa agcaataggt
    15601 catgcttatg cacattcaga aataaataag gcaatgactg agtacaacaa aaatagaact
    15661 ggtggagtaa gagaaacagt aatacttgaa gaagatgtaa atgctgctcc aatagtacga
    15721 ttagtaaata acatactaga aaatgctgtt agaatggaag caagtgatat acatatagaa
    15781 caaagtgaaa attacatgcg tgttaggttt aggatagatg gaatgttaag agaatatatg
    15841 aggatgaact cagcaccata taaggcagta atcagccgta tcaagataat gtcagatata
    15901 aatatatctg aaaagagaat accacaggat ggtagaatct atttaaaagt agataataaa
    15961 cccatagatt ttagggtttc tacaatgcca actaacaggg atgaaaagat agctatgaga
    16021 gttcttgata agagtaattt tatggttagt aaagaagtac taggaataga cgaacatggc
    16081 tctaagatat atgatgaatt gataaacact ccttatggtc ttatattggt tgttggccct
    16141 actggaagtg gtaaaaccac tacattgtat tcaatgttaa atcaactaaa tacagaaaat
    16201 agaaatctat taacaataga agaccctata gagtatgaat tacctggtgt aaatcaatct
    16261 caaataaatg aaaaggcagg gcttactttc gccagcggac ttagagcttt tatgcgtcaa
    16321 gacccagata taataatggt tggagagata agagatactg agacagcaga aatagcaatt
    16381 agagcttctc ttacaggaca tttagtttta agtacattac atgcaaatac agcagttgga
    16441 gcaatttcac gtcttttaga tatggatgtg gaaagttttt taataacttc atctgtttta
    16501 ggtgtaatat ctcaaagact tacacgaaaa atatgtgagc attgtaaggt ttcatatgaa
    16561 gcagatattg gagaaaaaaa ggctcttggt attgatgtaa atgaatcagt aactatttat
    16621 agaggaaaag gttgtgaaag gtgtaataat actggatata aaggacgtct aggtatattt
    16681 gaaatgcttg aaattactcc agaaataaaa gagttaatag attcatctgc aaatcaaaga
    16741 gaaattttaa agatggctag aaaacaaggt atggttagct taaaagagga tatagttaaa
    16801 aaagttttaa atggaaagac aacagtagaa gaaatgatta gaataatttt gatgactgat
    16861 taaaggatga tattatgaaa agttttaaat acacagtaat aaaagaggac ggaaaaaagc
    16921 aaactgatgt gattgaagcc aacgatttta atgaagctag aaatcttctt agaaagagaa
    16981 atcttagagt tattgaaatc aaagaaagca aaaataaaaa tataagatta tctagtagaa
    17041 agagaaaaaa agacttagga gctgaccaga taagccactt ttgtagacag tttgctatta
    17101 tagtatcttc tggtataaat agtatttctg gattagaaac attagctaga aggtcaacta
    17161 acattacatt aagagaagaa attaatcgta tagtggcaga aataaaagta ggttctacta
    17221 ttgcagattc aatgctttca tcaaaatcaa agtttccaaa gttacttggt gctatggtag
    17281 ctacaggtga agctacaggt aaattagatg aagtattaaa aagtatggct tcattttatg
    17341 caagtgaaca cagggttaaa caaaagctta gaaatgctgc aacatatcca ttaatagttt
    17401 tgatttcttc atttataatg atttttatat ttacaacatt tatggtgcct aagatgatta
    17461 attcaattac aacagtagga gcaactcttc cacttataac aagaattgtt atggggtttg
    17521 gtatgtttat gaagaagttt tggataatag tacttttagt tattatatta tttatctatc
    17581 agtttaaaaa atatctaaaa acgtcagttg gaagggctca taaagataga gttataaata
    17641 agatacctgt tttaggaaaa ggaataaatt gtatggtagc tacacgattt tcaagagcat
    17701 tgtatctatt tgtatcaaca ggatacccat tagttcaagg gcttgattat attatagata
    17761 gtgtaaataa caccatggca gagaagattt tagcatcagc taaagatgga ataacaagag
    17821 gagaaggctt agcagaaaat ttagagagat atacgtattt tgactctgtg ttggtacaga
    17881 tgatagctat aggggagcag acaggggaat tagagaatat aagtagacag atggcagaat
    17941 tttatgagta tgaatccgag atttatttaa ataggatggc ctcaatgata gagcctgttt
    18001 taataattgc agttggtatt atagttgcga tactggttgt atcaatattc atgccaatgc
    18061 ttagtatata tgatgccatg taggagggaa aggacaaatg aacaaggttt atgtatcata
    18121 ttatggcgat tctatttcca tagttgaggg cagatataaa cacgataagg ataagtttta
    18181 tgtaaaaaat tttaatgtgt tttcaataga ggatattgca caagattttt ctaaggaaaa
    18241 gtatgctcta ttgagatacg cattagaaaa tgttaagtta aaatcaagac atgtagtttt
    18301 ttgtttaaat acaagagatg taattttaaa accccaaaaa cttcctaata tggacaaaaa
    18361 ggatttagac ggatttatga agctagagat ggacgaaatg atggctttgg agtcagatga
    18421 atatgtattt tcgtatgaag tatctagtga aacacctgat aattcagatg gcgatgaatc
    18481 aagtttaaat cttattattg gagctataca aaaggaagag gtaaatgcaa ttgtagagat
    18541 aatgcatgaa tttgatttag tattagatag aatagataca ttatcaacag cttacttaag
    18601 aatgcttaaa aatttagact acaatgatat aatggtaacc aatataagtg acaatagcac
    18661 aattataaat atatataaga aagatacttt atttattagt gataatatac ctataaagat
    18721 aactgctaaa gatatggaca tgcaattatt aaatgtagta gaagaatcaa aaggtcttat
    18781 gaattactat tcgtcaagaa actttggtaa ggttacggat actatattaa tattaggaaa
    18841 aagatattcc aataaattaa tttatgaaac ttttagctct gcattttcaa gctatgtttc
    18901 tcaaggcctt actgagataa tggatatatc tgatatgata actggagata tagatgaaga
    18961 tgatataaat tcaattgtag agcttttagg aagtatgata gaacctaaag ggaaaagaga
    19021 ttttgaaaat ataaactttt taccaatgga tatacttaga aaacagataa aagctaaaca
    19081 aatgaaaaac atactgaaaa ttgctccaat agttattttg attcttgcta taccttattt
    19141 atgtttaatt ttttcaaata atttagcaaa taaggaactt gaaaatgtta atggtgaaat
    19201 aagaaatatt gaatctgcat attatcagat tggaaatata gaaaaaatga tacagagtaa
    19261 aagcgaagaa ataaaggtat atgatatgtt aattgggaaa aaggtaaagt gggaaaattt
    19321 attggatgat atagataaaa atactccaag taaagttgaa ctaacaagtt tatcaacaac
    19381 ttatcaaaat cttggaacta ataataaagc tgataattca aaaagtaata agactacaac
    19441 agataaatca agtaacaatg aaacaaaatc aagtacgaaa agtgatagta atggagataa
    19501 aacagataat gatttaaatg aagataagac tacaaatgtg tcagaagcat taactggaaa
    19561 tagtgagact aatagtaata acacagataa agatgataaa actccattgt atgataagat
    19621 accaaatgtt ataaatatag aaggaaatgc taaagattcc tcttatgtag gtcaattttt
    19681 atataaatta aagggattat actatttcaa ggatgttaaa ttacatagca taaaaagtaa
    19741 tgcagaagat tctaactata catttagtat gacattatac ctgaaagaag gtgtattaac
    19801 taatgaataa agatatattt aaaaaagata taaaagatat atttaaaatg gaagttagtc
    19861 taaaagggat tcttataaag gttataattt tattagctat tttatcatat ggttattttg
    19921 gactttaccc tcaatatcag aaatatattg ctacaaaaaa ttctgtaaaa caggctactg
    19981 aaaaattaaa tatatataaa gagaaaactg cacttatgcc aaaatatgaa aaacaacttg
    20041 aagaagttga aagtgaatta aaagaaaaaa gcaaaaatct tttatatgat atgcaagatg
    20101 gtcttttttt aataggttta gataaaaaaa tgaaggctta taatattgac ttaaccagtt
    20161 atgacgttgg aaatgttaaa gaatacgaac atttttatgg tatatcaaca acaataagtg
    20221 ttagaggtga ttatagaaat gtcaaaaaat taatggctta tttagaagaa caaaaaaatg
    20281 ttactcaaat actagattat acaatgactt catatataga accagaaaaa gaagaggaag
    20341 aaacaacaaa caataaggat ttaattaaaa atgtgaacat aaataaagat acaaaaatat
    20401 actgggtgga aggcgaaaat aagtggcatt tatataagga ttgtccattg ataaaagagg
    20461 gagagattct aaaaaatgac aaacttttag ataggtctac taattatcta tgtaatactt
    20521 gtgtacttag agctgcaaca gaaaaagaag caaaaaaaga agtcgtagct ccaaaggcag
    20581 aaggtgtagt agatgctaca tttaaattta taatgtacac taaagagaat actacaatga
    20641 tacttgatac agataatcca tcttcatgga gaccaggaaa atataatcca tttaagtcta
    20701 gtgcgaaata aagctaagta agctttaaag ataggtgatg agtttgtaca aaaataatga
    20761 aaaaggtctt actttattag aagtaataat agctgttttt atactgacaa tagttttaag
    20821 tatttcttat aaagtgttca atggtataac atcagcagta aaaaagcaac agattattac
    20881 agatgctcaa gtaaatatta atctaattaa taagtatcta aatagagatt tggaaaactg
    20941 taaagaacta actaaaactg gttcaggtaa taattatgaa tacaatatag agatgccaga
    21001 taatgttgta aaatatgaag tttctataga aactaaaaaa aatacagagg tatattctgt
    21061 aacgagaatc caaaacaata caattgatac agaaaatgaa gttagagaag agataatcta
    21121 taatcaacca ctagttcaaa acaataaaga aatgaaggaa acaccattta aaatagaaaa
    21181 gcaaactggt aaatctattt atactgtaag tatatactat aatgaatcag tgcaagaaag
    21241 tcataaaaat agcaatttaa ataacaaaac ttatacattt gatgttatgt caagaatagg
    21301 ataataagaa tttattaaaa aataagtagg ttgataaaat atgagtaaaa agaatagtga
    21361 aagaggattt tccttaattg aagtgttagt agctatggct attatgggaa ttgtactatt
    21421 tgcatttttt aatattatca atactaataa caaagcaaac actaaaaatg acacagatat
    21481 aacttcctta aactatgtcc aaagtgaaat agaaaatcta agagaaaaga taaaaagtgg
    21541 agaatttgat tttgatagtt tagataaact agaagatgga actgtcgtat atgaaaaatt
    21601 aatagataaa tcaaaaaaag ttgtatatga taaagttctt agtgaaggtg atgtgagctt
    21661 atatgatact ccatatgaaa agattacaac aataaaagat gaagatggta atttaatcga
    21721 caaaggaaat attacaaata agataaagac tattgtagaa gataaaagtg gtcagatata
    21781 taaaatagct gtaactggga aaagcatgaa tgactattca agtaaaaaag aggttaaaat
    21841 tgtaactgaa atatttaaag ataaataatt ttacttgggg gtgagatttt gaggaagtgg
    21901 aataaattta aaagtgaaag aggggcagct ttagttttag ttttggttgt agtagcattg
    21961 ctttctattg ttggattgat attttcaaat cagatagcta atagaataaa atctacaaag
    22021 actactaatg aaggtataca agcaaagtat ttagcagaga catgtgtgga aaatagtatt
    22081 gataaagcat atgaaaaatt gtatgatgag cttgagaaaa tggataatga gtttaaaagt
    22141 gaaaatcaag aaaaatccat atctaggtca aaattaagag atatatcaga tgaagatttt
    22201 aataatcaag atgaggaaaa tattgaagca gaaagacttg gatatatgaa taatataaat
    22261 ttctatttaa ataaagcttc ttcagacttg gaaaaagcta gtatggaatt gaaaaagcta
    22321 tatgatttag atatgttgga ctatagagac attgaatatg tagatgcaaa tattatttca
    22381 catagagatt ctatcttaga aatttgcaag aattatactt ctggagatat tagtaaaata
    22441 aatgaatata ttctgaaaga agatatagac tcaactacat tgattgaggc aaagttagtg
    22501 aataatgata ttcttttaaa aatgttttta gaagaaaata aaattgaaaa tgaacattta
    22561 aattcagcct tttcacatac ttataaggca ttagataaca tttctttggc gatgcaaaat
    22621 atgattgagt atagacatac gtttcatata gatgagccaa aagtagaagt atctaatggg
    22681 attccagatt ctcaacaata ttatgaacta atacaaaatc ctataataaa tagtatggag
    22741 tatatctgga atagtaaatg ggatacatta gaaaaactat tggaaatttt accaaatcaa
    22801 actcaaggat ttaattcttt aagagttcat ttgagaaata atgtaagaaa atttgagaag
    22861 ttaagcgata atatatctag tggtaaaaaa aatacagcaa aaaatttctt aaagtataaa
    22921 gagttattgt atgaaatatc agaccaatgt aatcaactaa aaagtatgtc ttatgagaaa
    22981 atacctgtta aatatgataa catggctttg ataacaactt ttgactatat acaaaatgag
    23041 cttttagcag agataaaatg cagattaaaa gaattaaaac cacaagaaat tgataaaaca
    23101 gaaggtatta ctataaaaat acctttttat aaggctgact acgacatgac aaaagaagga
    23161 tggcctaaat taaaagaaaa tggttcaggg gcggagttaa gcttaatggt aactggtgat
    23221 aaagatggaa taaaagaagt agaagttaca gatggtaaaa aaaatattat tggtttaggt
    23281 gttgaagaaa attcaaattc aaagtataag gttgacgcca ttgtaaactt taatctaaat
    23341 atagatacaa atgtagttgg aaattatgat ataaaagata aaattttgat taatcatgat
    23401 ataagttcat acaaaaaagt aaactaaata taagaagcaa cttagtataa ataaataata
    23461 aaataatttt attatttact actaaaaaaa taagtaaact aatagaagat atggagagta
    23521 aatattatgt atatttatga tttattagag caaggtataa gactaaacgc atcagatata
    23581 cacataacag ttggaacaaa tcctgtagct agagtaaaag gtggatttgt aaaactaagt
    23641 gaacaaatat tgacatcaga agtaacaatg caaatggcaa aagacatagc aggggaatct
    23701 atgtttaaag taatagaaga acatggagaa gctgattttt cagcttcttt aaaaacagga
    23761 gaaagattta gggtaaatgc atataggcaa aaaggaaatt acgcaatagc aataaggact
    23821 ataacagcag aaattccaac atttgaaaaa ttaggccttc cagagagtat aaaatctttt
    23881 actgaaaagc ataaaggatt agttttagtt acaggtccaa caggaagtgg taaaagtaca
    23941 acacttgcaa gtatgataaa tataataaat gaaaaacaac aaaaacatat aattacactt
    24001 gaagacccga tagaatatgt acatcatcat aaacaaagtt tagtaaatca aagagaagta
    24061 ggaactgata cagaaagttt tcattcagcc cttagagcta ttttgcgtca ggacccagat
    24121 gtaatactaa taggagagat gagagaccct gaaactgttt caatagcatt aacagcagca
    24181 gaaacaggtc atttagtatt ttcaacacta catacagtgg gggcagctaa gacaattgat
    24241 agaatagtag acatgtttca accatcccaa cagcaacaaa taaaaacaca gttatctaca
    24301 gtttgtgaag gtgtggtatc acagcagtta cttcctacag cagatgggaa aggcagaata
    24361 gcagcaattg aattgatgtt tgctactcca gcaataaaaa acctgataag ggaaggcaaa
    24421 acatatcaaa ttccaaacat gatacaaact ggagttaaag taggtatgaa aactatggac
    24481 caagatttaa tggaacttta taaaaatgga aagattacaa aagatatggc acttagtagg
    24541 tgtactgacc aagagtttat gacaagaatg ataggtggag taaactacaa tggatattat
    24601 aatcgttaat ctatatgtat ttatggtggg tatagtgttt ggaagtttct ttaacgtctg
    24661 tatttttagg attccagaag gtatatctat ttctagacct ccttctcatt gtatgagttg
    24721 taatactaga ctaaagccaa tagacttaat accagtgtta agctatttaa tgagtggcag
    24781 aaagtgtaga tattgttatg agaaaatttc tagtagatat gctatagtgg aattaattac
    24841 aggactttta tttttaacag tatttatgtt atatggaata tctacatcaa ctatttatta
    24901 tttggttctt gtgtctttac tgattataat tacatttata gatattgacc actttataat
    24961 accagataaa atattggttt ttggagctat attttctatg atttttaacc ttttatttaa
    25021 agaaattcca gttaaggatt caattctagg tgtttttatt tgtggaggaa gtgtctggat
    25081 tatagttctt ttgattgaat ttgtaataaa aaaagagtgt atgggtggag gagatatcaa
    25141 actatttgca atgctaggtt tttacatggg tgttaaaaat ggtcttttga cagccttact
    25201 aagcgtttat gtgggagcag tctatggaat ctgtgtaata attataagta gaataaaagg
    25261 aaaggaatat aattctgtta taccatatgg accttttata tctattggag cattaataac
    25321 aatcttatgt ggaaagcaat tacttgaact ttatttttct atggttatat agatgatagt
    25381 caagttaaat aaagttttaa tttaaaaaaa tgaattaaag tatttaataa ggacaatatt
    25441 attattaacc ctattactaa agtgctacaa actatggagt atataataat aatttcctta
    25501 ttttttgcac atttgataaa taaatctcta tgtgtttttt gtgatagaga aaagaaaaat
    25561 ttatacaata cgttttttat ttacataata actgtagttt tatctgtatt aatatatcta
    25621 aaatttggga ttagtgtaga atgtgtacaa tatatggttt taattccatt tttaataatt
    25681 atatccattg tagattacta cacaatatat gtatatgata taacaatagt tagtggtata
    25741 atagttcaag gtataatttt tttggtcttt ttcaatatag ttcaatttga ttatataaat
    25801 catatatttg gactattgat aggctttatt ataccttata ttattgctaa actaacaaaa
    25861 ggattaggct atggagatgt aggtctctat agcttatgtt gctttgcatt gggaaataat
    25921 tatgggtttt atgtgatagt tttatcattt attttagcgt ttttgttttt tattatttta
    25981 tacataacta aaatatttaa agatgtagat ataagaaagc aaattccttt tacacctttt
    26041 atttccttgt caacaatttt aataatattt acagagtata atatatttaa tttttattat
    26101 aatttgttat acaaatttat ataacaggag gtaagaaatg tacgtagtag tagggttagg
    26161 taatccagga aagaagtatg agaagactag acataacgtt ggatttgatg ttatagatat
    26221 tcttgctaaa gaatacaata ttagtgttac taaaataaag cataaagctt tgattggtga
    26281 agggagagta ggtactgaaa aagtattatt agttaaacca caaacttata tgaatttaag
    26341 tggagaaaca ttaatagata tatataaata ttataaggtt gatttaagta atatagtagt
    26401 agtatatgat gatatagatt tagaagttgg aaagatacgt ataagaaaaa aaggaagtgg
    26461 aggaactcat aatggcatga aatctataac taagtgccta ggttcaaatg actttccaag
    26521 agtgagagta ggtgtatcta aaccagaagc tggtcaagac ttagcagact ttgtactttc
    26581 tagatttaga aaagaagaat ctgataacat aaatgaagca ttagaaaaag cggcagatgc
    26641 tattgactca ataataagag aaaatataga tatgtctatg aacaaatata atggatagtg
    26701 agagggagat ttaaatgaat gatgtttttt tatatccttt gcaaaattca aaagagtata
    26761 aagatataat aaactgtata aaaaatacaa aaggctcatt attggttaat ggacttttac
    26821 cagtacaaaa acctcatata tcgtattcta tatttaatga tttatcacga caaatgattt
    26881 ttataacaag tagtgattta gaagcaaaga aagtatacga agatttaagt ttttatatgg
    26941 aagataaagt tgaatattta ggttttcaag atatttattt ttatcacttg gatgcaaagg
    27001 acagaaacga agaagcgaag aaattaaaag tattgttaaa actagcaaat aaagaaaaaa
    27061 tcattttagt aacttctatt gaggcagttt taagaaaata cataccaaaa caagttttgc
    27121 tcgatagtgt atctcattat aaggtgggag atagcttaga cttagaaaag ttaactgaga
    27181 aattagtttc attgggatat gaaagagttt caaaaataga ggggtttggt caatttagta
    27241 taagaggtgg aattattgat gtattttcac ttgaatatac aaatccaatt cgtatggaat
    27301 tatttgatga tgagatagat tccatacgaa cttttgacgt atattcacaa aaatcaattg
    27361 ataaattgca acaattctct ataactccat ctagggaatt tatttatccc gaaaaaacta
    27421 cagatgcatt agttaaatta aaaaaagaaa caactaaaaa tactgatgaa ggtacatttc
    27481 aaaatataga ttatatatca agtaagactt attttgaagg tgtagagaac tatatagatt
    27541 atatttatcc agaagaaaac aaaagtatat ttacttattt agcagatgat gcaatagtat
    27601 ttattaatga tataacgaga cttaaagaaa gatgcgaaaa ttatataaat gagtttagag
    27661 aaaattataa attaaatctt gaaagaggtc tggctattaa aaaccaaggt aaattgttat
    27721 atcactatac agatttagag tacttagtaa aagacaagtc tgtggtatta aactcgctat
    27781 tgccaaagtc tataaataac ttttctataa aatctataat aaattttgaa agtagagaag
    27841 ttcctacatt taatggaaag gtagatttgc ttgtagaaga attaaatcgg ctgaaatata
    27901 atggatacaa aataatttta gcaacaaata ccttagaaag agcaaataaa ttaggaaaag
    27961 atttattgga caaaggctta gaaacgacca tttctaaaga tagagatatt gaaattaaat
    28021 cttcacaagt tataattgta cctgcacaca taaacagtgg ttttcagtat aaatctataa
    28081 agtttgttgt tataacagat aacgaaatga taggtgttta taaaagagcg tctaagacta
    28141 gcaataaaaa agttaaaaag ggaaagaaaa tagaatcctt tttagattta agtgttggag
    28201 attatgtagt ccatgaaaat agtggtgtag gtagatacac aggaattgaa caaattacag
    28261 tcaatgctat taaaaaagat tatatgaaaa tagtatatca aggtggagat aatctatatg
    28321 tgccaataga ccagatggac aaagtacaaa aatatattgg tgcagaagta gaaaaagtta
    28381 agttaaataa gcttggaaca aatgagtgga cgaaagcaaa agcaaaagtt aaaaaagaaa
    28441 ttgaagatat gacaaaagac ttgattgagc tttatgcaaa acgtgaaaaa atacaagggt
    28501 ataagttttc aaaagataca ccttggcagg cagagtttga atcgttgttt ccatatcaag
    28561 aaacagaaga ccaattaaag gctatagaag aaactaaaaa agatatggaa tcaagtaagg
    28621 taatggatag gctagtatgt ggagatgttg gttatggaaa aacagaggta gctattagaa
    28681 gtatttttaa agcttgtatg gaccaaaaac aagttgctgt attggttcca acaacaatat
    28741 tggcccaaca acattataat acattcaaag agaggtttga aaattatcct ttgagagtag
    28801 aagttttaag taggtttaaa acaccaaaac aacaaaaaca gattattgaa gatgctaaaa
    28861 aaggattagt agatatttta attggaacac atagaataat atccaaagat ataaatttac
    28921 caaacttagg tcttgtagtc attgatgaag aacaaagatt tggagtaaaa cataaagaag
    28981 ctttaaagaa aattaaatct acagtagatg tacttacact ttcagcaact cctataccaa
    29041 gaacattaca tatgtcttta agtggaataa gagacatgag tgttatagaa gaacctccac
    29101 aagaaagaca tcctgtaata acatatgtta cagagagtaa ggaaagcgta attcaagatg
    29161 agatagagag agaattaagt agaggtggac aggtattttt tgtatacaat agagttgaac
    29221 acattgaaga aatggcaagt atgattcaaa aactcgtacc agatgctaga gtggctgttg
    29281 cacatggaag aatgacttct aaatcattag aaaatataat cctaggattt ttaaataaag
    29341 attatgatgt actagtctgt actacaataa ttgaaactgg tatggacata tcaaatgcaa
    29401 atacaatgat tatatatgat gcagataaaa tgggattggc tcaactttac caattgagag
    29461 gtagggttgg aagaagttca agacaaggat atgcatatct tttgtatgaa aaagataaaa
    29521 ctttaagtga gatagcggag aagagattaa aagctatcag agaatttaca gagtttggtt
    29581 ctggttttaa aatagcaatg agagaccttg aaataagagg agcaggaaat attttaggtt
    29641 ctcaacaaca tggtcatatg gcagttatag gttatgattt gtacgttaaa atgcttaatg
    29701 atgcaattaa aaaagtcaaa ggtgagccta ttgtggagga aattgatgtt gaaatagatt
    29761 taagtgtaaa tgcatacatt ccagataatt atatcaaaga tgagttgatt aaaattgaaa
    29821 tgtataagaa aatagcttct attgaaaaca aagaagacat gttggatata caagaagaac
    29881 ttgaggatag attctctgat atacctaaac cagtccagac acttcttaca atagcttata
    29941 taaaatcgtt atgtaagata ttaaaaatag aaaagataag acaattaaaa gatgaaatat
    30001 tattagttcc aataacaaag tatagaacta aacagaaaat aggttataat atagttacag
    30061 aattagaaga attattagaa aagatgtgca aggtaaaata acttgtaaat ttaagaaggg
    30121 atgttttaaa tttgaaaaaa gtaataacat tggtaatagc tatgattttg gtagtatctg
    30181 tgactgcttg cagttctagt aaaggagaaa ctgtggcaac agttgaaggt acaaagatat
    30241 catcagatga atttaaaaaa actatagctc tttataaaga ttctatggaa caaacttatg
    30301 gaaaagatat atgggataag gaagttgaaa agggtgtaaa atacaaggac aagtttaaag
    30361 atttaattct tgaccaactt ataactacag aagtaatata ttctcaagct aaaaaagata
    30421 acttacttcc taaaaaagag gatgttgaaa aaagttttaa agaattaaaa gacgctatgg
    30481 gtaaagatga aaaatataaa gaacaactta agaaattagg tatagatgat gagtttttaa
    30541 gagaccaaca agagaaagat ttagcaatgc aaaactatca aagtaacttt gctaaaaaga
    30601 ctaaaatatc agatgaagaa atgaaaaaat actatgatac acataaagat gaatttaaaa
    30661 aagatgaggt agaagcttct catattttat tgaaaacagt agatgataat aacaagccac
    30721 tttcagataa ggaaaaagct gaagctaaga aaaaggctga agaggctctt aaagaagtta
    30781 aatcaggtga ggattttgca aaagtagcaa aaaaatattc acaagatact tcagcaagtg
    30841 atggtggtaa attaggattc ttttcaaggg gtcaaatggt tgctgaattt gaagatgctg
    30901 ctttttctat gaaaaagggt gaagtatctg atttagttga aactcaatat ggataccaca
    30961 taattaaagt gacagacaga ataaatgagc aaacatcatt tgaagatgct aaagaaacta
    31021 taaaagacca actgttgaaa aataaatatc aagaacaaat agaaaaatta actaaagaag
    31081 ctaaggtaga aaaagatgaa aaagtaataa ataaaatcac aatctaagac ttaaaaaaag
    31141 agtaatatgc ttaaaaaagc tatatctgga taagatatag ctttttttgt atatttttct
    31201 taacaatggt aaaaataaac ttaaagtaat ttatggaggg attaaaaaat atgaaagcaa
    31261 caggtatagt tagaagaata gatgatcttg gaagggtagt tatcccaaaa gagataagaa
    31321 agacattaag aattagagaa ggagaccctt tagagatatt cacagcaaaa gatggagaag
    31381 tcatacttaa aaaatactct ccaataggtg aattaaatga attttcgcaa gaatatgctg
    31441 aaacattagc tgaagtacta ggatatggag tgttgataac agacttagat tctataatag
    31501 ctgtgtctaa gcttcctaaa aaagattata aagaaaaaag tataagtgat gagttagaag
    31561 caataataag tgaaagaaca gtaaaatatt caaaagataa aaacatttta ccattattca
    31621 aggaagacca aaaagaatat actagtcaag ttatcatgcc tataattagt gcatctggtg
    31681 actgtatagg ttcaattgta gttgtttcaa aggataaaga tacaattgca gaaagtgaag
    31741 aaaaatcttt aaaaattgct gctaactttt taggaaaaca agttcaataa ttttatcata
    31801 aagacctttt acatgaaggt cttttgaagt ttttgtgtaa aatattgaat atagcattaa
    31861 aaatatggta tattaaagtt tgttatataa acagtatgga ggttatgtta tgaacaataa
    31921 caaaaataaa gactcctttc taaaaggagc ccttatactt gggctagcag gtgtaatcgt
    31981 aaaaataatg ggtggatttt ttaggattcc ccttggaaac atgattggtt cagaaggaat
    32041 gggatactat caggctgctt atcctgtgta tactctattt ttaacattag ctactgctgg
    32101 atttccaaca gctgtggcaa aattggtttc ggaaaaagtt gcaattggaa attttaaagg
    32161 tgcaaataag atatttaagg tatcacatac agttttattc attaccggta taatatcatt
    32221 ttgtatatta ttttttggag ccgactacat agtaacaaac gtaatgaaaa atccaggtgc
    32281 gttatactct atgaaagcca ttgctccagc attactattt gttcctgcaa tgtctgcata
    32341 tagaggatat tttcaaggga gacaagatat gactaagatt gctgtgtctc aagttgcaga
    32401 gcaatttttt agagttgtat taggtcttac tttggcttac ttgcttatga agagtctagg
    32461 tcagactttt ggtgcagcag gtgctatatc aggggctaca ataggttcaa tagcatctat
    32521 gctttatttg gtgtttgctt atatgctagg gagaaaagag cgtagagcag agatagatgc
    32581 aagccaaaga tttaaagatg aaagagtgtc ttatattttt aaaaagcttt taactgttgc
    32641 cattcctata acaataggag catcagtaat gccacttgta aatatgatag ataatgttat
    32701 tgtaataaga agacttatgg aagcaggctt tacatacaaa gtggcaaatt ccatgtttgg
    32761 tcaattaaca ggtatggcaa tggccacaat aaatttacca gcagtcataa caactgcaat
    32821 gagtatgagc ttagttccag caatttctaa agcatatgca ttaggaaata agtcaaaagc
    32881 tagaaaagat actaagagtg cagttaaggt aactttactt atagtattgc cttgtgcgtt
    32941 tggtatggct tctttagcaa taccaataat gggtctttta tttccacatg aaccatcttc
    33001 tgtaggaact attttgttta ctcttactcc atgtgtatta tttttaggat taatacagac
    33061 attgactggt ataattcagg gaatgggaaa accaatagta cctgtaattg ctttatgtgt
    33121 tggtatgcta tgtaaaattg taataagtta tactttaaca ggtataccag atattaatgt
    33181 actgggttca gcatttggta cagttacagc atactttgtg gcagccatga ttaacttact
    33241 atatgttaaa aaacacatga atgtaaactt ttctaaaaaa gaatttataa taaagccatt
    33301 tataacagtt atgacaatgt ttataatggt taagttgagt tatggtgctt tagttggatt
    33361 cttaggaaac agcatatcta caataattgc aatatgtgtt ggtggaattg tatatgtagt
    33421 agtaatactt ggaataggtg gaattaaaaa agaagagatt ctaactatgc caaaaggtga
    33481 taaattatat aaattgttaa aaaaagttaa acttataaga taatatagga atatatatgc
    33541 aaataaagga aatactatac aaatagtaaa tctccttatt tgtataataa tataaaaata
    33601 aagtgaaatt tgctaatagg agtgatgtta tgggtaagat aagtattgta ggtcttggac
    33661 caggagacta ttctcttata agccaaggtg tgttagaatc tttaataaat agccctaaag
    33721 ttttttttag aacagagaaa catccaataa ttgataaact taaagaaact atacaataca
    33781 cttctttaga ctacttttat gaaaaagagg aaaattttga taatgtttat ttgaaaatat
    33841 ctgaatttat catagaagaa tcagcaaagg gtgacctagt gtatgctgtt cctggtcatc
    33901 caagagttgc agagaagact gtaagtataa tagagagatt agccaatgaa aagcacatag
    33961 cagtagaaat attgccttct atgagctttg tagacgctat gtttaactat ttggctatag
    34021 acccatctga gggatttaaa ctggtagatg cttttgaaat agaaaattca tatatagact
    34081 tggatacaag tatgataata actcaggtat atgataaatt tatagcatct aatgtaaagt
    34141 taaaacttat ggaacattat gactataatc aagaagtatg tatagtaaat ggagctggtg
    34201 tcaaagattt agaaagtaaa aagtttataa agctttgtga attagacaga aatgaaaact
    34261 tttttgacta tttaactagc ttatatatac ccaaaagttc taaaaagatg tataatacag
    34321 tacatgactt agaaataata gttaatacac ttagaagtcc atctggatgt gaatgggata
    34381 aaaaacaaac acatcaatca ttaaaaaatt ctgttataga ggaagcatat gaactttgta
    34441 atgcaattga taataatgat atagatgaaa tggtagaaga gttaggtgat gttcttttac
    34501 aggttatatt ccattgtcag ataggaaatg aggagggcta ctttgattta aaagaagtag
    34561 taaatggcat atgtaaaaaa ttaattcata gacatcctca tatatttagt ggtgcaaaat
    34621 tagatatgtc taagtttgag aaaacttggg aagaattaaa gaaagaagag aaaggcgaaa
    34681 gtactataac agatggcctt agaaggatac caaaacattt accagctctt atgaaggcta
    34741 gtaaagttca acataaagca gcacttgtcg gctttgattg ggacaatgta gaagatgtat
    34801 ttaaaaaaat tgaagaagaa tatcaggaat tacttgacga acacagggtc gggaatataa
    34861 aatacataaa ggaagagcta ggcgatttat tattttcgat agtaaatcta gctagatttt
    34921 tagacataga ctcaggagaa gccttgaact gtactacaga aaaattcata aatagatttg
    34981 actttataga aaagagtgca atgaacttga ataaaaaatt agaagacatg acacttgaag
    35041 agatggatga gttttggaat caggctaaaa aacaataaaa ttgtggaagg aaatttgtca
    35101 aaaatctaga acttttatca caaagataga agtctatatt ataaacattt ttaggaggta
    35161 aatatcgtga ataaagctga attagtatca aagatggcag aaaaaagtgg attaacaaag
    35221 aaggaagcag aagctgcgtt aaacgcattt atgagttctg ttcaagatgc actagtaaat
    35281 aatgaaaaag ttcaattagt tggatttgga acatttgaga caagagaaag agctgctaga
    35341 caaggaagaa atccaagaga tccagagcaa gttatagata taccagcttc taaagcacca
    35401 gttttcaaag ctggaaaagg attaaaggat ataataaatg gataattaga aatcttgtga
    35461 aaaagtgtgg ataaatttgc cacacttttt ctatagaacc aaggaaggtg ataattatga
    35521 gattagataa atttttaaaa gtatctagga ttataaaaag aagaactgtt gctaaagatg
    35581 catgtgataa agggattgtc actataaatg gaaaagttgc taaatcttct tcagaggtga
    35641 atattggaga tacattagaa atacaatttg gagagaagaa gatgaagttt aagataaatg
    35701 aagtaaaaga gcatgtatta aaaaatgatg caaaagaaat gtacgaaatt ttagaataaa
    35761 attaatagtc ctctagtata ttaatagtag tattattgaa atatttactg gggggatatt
    35821 atggaacaga acataacttt gaaagataga tcaaagctgg ttatttcggg agtcgagcac
    35881 atatactctt ttaatgacaa aagagtagag ctcaaaactt ctgtaggcga gatggtaatt
    35941 gaaggcgaaa acctagatat gagcaagcta agtatagacg aaaatataat tagcatagat
    36001 ggaactataa attctatggt atatgcaaag cctaaaaagc cacaagagag tttcttgaaa
    36061 aaggtattta gatagatgat accttttact caagatgtaa gcatttttta tgctactatt
    36121 tatggtggaa tactcatagg ggtgttgttt gatttttata gagggctaag agggaatttt
    36181 aaattcatca attattttgc tataatattt gatgtattgt tttggtttct agctacggtt
    36241 ataatattcg taacaataaa ccttacagaa ttttttgatt taagatatta tcattttgtg
    36301 gctttgttta ttggttttat tttatactat aacaccataa gtaaaattgt tttatcaatt
    36361 atcaataaaa taattcgttt tgtgagaaat tcttttaaaa aagttacaca ttacatagtc
    36421 agttttttaa ataatttgta ttacgttata atctattcac tacacctact atttgatatt
    36481 atattttata taccaaacat atttatagca acaagaaaaa gtataaaaag aaaatcaaat
    36541 aaaaaattaa agaacaagaa aaaatcaaaa cctaagaaga aaaaaaataa aactaaaaaa
    36601 agggtgtagg aaaaatgaac ttaagaaaaa aattttcagg acagattatt gttatcagtt
    36661 tatttttagg aatatcaatt tttagtatga tgacaggatt tgtatttgag tatacaaaag
    36721 ctaaggaata taaaaaagaa atagcaagtc taaataaaca attaaaaaag actgaaatac
    36781 aaataaacag cttaaagaaa gatgaaaaat catatgaagg agacttagag gatatagcaa
    36841 gaaaaagatt gaatatggta aaaccaaatg aaacagttta tgtagacata aatcgttgat
    36901 taaaacttgc taataagttt acagatgtat tgattttaaa atttaagatt taataaaaaa
    36961 gtcattcagt taaggatggc tttttgtttt tattgaggag ggaatttcat aatgaaagta
    37021 ggagctatag atattgggac aaactcaatg agattattga tagcagatta tagagatggg
    37081 aattttataa acagaaaaaa atttgttaat acaactagaa ttggttatgg tgtggatagt
    37141 aaaggatata taaaagaaga tgctatcaaa agaaatataa aagcattaaa agaattttct
    37201 gataagtgta atgatgaaaa gtgtgaaaaa atttattgta tggggacatc tgctcttaga
    37261 gacagtaaaa atggagatta ctttgttaaa ttagctaaag aagaaactgg aatagatgtt
    37321 gatatagtta cgggaataga agaatcaaat ttgggcttta agggtgtttt agaaggaata
    37381 caaggtgatg atactattat tgttatagat attggaggag gttcaacaga atttattgta
    37441 ggtgattcag aaggaattat atttaataaa agtgagaatg taggtgctct taggatgaca
    37501 gagaggtttt tgaagcacga tattattgaa gatgaagagt ttgaagctat gagttcattt
    37561 atatatggtg agattgataa aacgttaaat catataaaag acaaaggatt aaaaactctt
    37621 ataggtatag gaggtacgat tacatcatta tcagcaatga atcaagaatt agagatttac
    37681 tctatggaaa aaatacataa tagtaaaata cacaaaaaag aaatagaaga aattctacaa
    37741 aaattaataa agatgacatt aagtgataaa aagaacttaa aagggctaca agaaaaacgt
    37801 gcggatatta taactgctgg tgtaaaaata ttgaatataa taatggaaaa attagaaata
    37861 gaagaaataa ccgtaagtga atacgataat ttggaaggct taatatgtca aaagtcaaaa
    37921 aaaatgtatt aatatttttt gatttttaaa accgattatg acaaaaaaaa ttactcctct
    37981 ttgttacaat tcttaatata aacaaaagag gaggtttttt tatgcaaaga agtgtagcag
    38041 ttgtgaagaa taagactgta aaaccaaatg tcataaaatt aataagcgga tatattgatt
    38101 ttactacggc ggcattcgct gtgatgggat ttttacttag taggtctatt atagtagact
    38161 ctgtagctcc tttaggaatt gcctttttta tatgtgcatc aaagattgat aagtacaaga
    38221 tacctgtatt catatcaaca ttattaggaa tattgttatc atctaatgat tttggaaatg
    38281 tagcaaaata tgctgtgtgc cttacaatat ttatggctat gaataaaaaa ttaaaattat
    38341 cagattcaat tgctaaggtt gcatttatag gtgcaataat actacttcca atctccatgg
    38401 gacaagcttt cttttcaaat aggtatgtat atggtcttct tatagcagga gtagaagcaa
    38461 taataatgtt tatatctata tatgtgtttt cttatgggat aggtttaatg gccaatgtca
    38521 acaataggat gtctgtacgg acagaagaag ccatatcaat aagtttactg ctaaccttta
    38581 gcataatggg aataggagat atatctctgt ttggaatatc aataaggacc attttatcga
    38641 ccgtattgat attgctgacc tcaatagtag gtggagctac tatgggagca tctagtggtg
    38701 taattattgg tgtagcatcc attttaaata atatgacatc agctgtttat atggggatat
    38761 attctttttc aggattaata agtggagcat ttaataaaat aaataaatat ttctgtatac
    38821 taggatatat tctaagttgg acaataatat acctatatac ttctggtatt acttctaata
    38881 tgatgcaatt aagagatata ctattaggtt gtttgatagt actagtattg ccagagcgat
    38941 tatttaataa gattgaaaaa ttaataaaaa gtaatgtggc ttcaaatgaa atcgtatacg
    39001 attatattat gagaagcaaa aacctaacta atagcagatt gaacagtatt tataaaactt
    39061 atgatgacct tgcagataca tttgataaga taagagaaaa agacaaagtg ctagaccaaa
    39121 gagacatagc taatgtaata gatatgattc ataatgatga atgtaaaagt tgtagtatga
    39181 gaagaatgtg ctgggagtca agatttaatc atacatacac tatgatatat aacattcttg
    39241 aaaaaattga ggagaaaggg gagttaagtc taaacgatat acctaaaaat tttagaaagg
    39301 aatgtatgaa accagagtca atagtgaaaa tatcaaatca ctattacaaa atgtttgtat
    39361 tagattatga ttggagtgtg aagttttcag aaagtagaaa gttaatagca aatcagataa
    39421 gaagtatttc aaaatccatt aaatccttat ctcaagattt agaaggcgac ataatgctag
    39481 atatagaaaa ggaaaaaaat atatatgaac agttagaaag atatgatata acagttgata
    39541 aagtaagtta cttaacaaaa agtaacagtg agtttgaaat atcaatagaa aagaaaactt
    39601 gccatgatgg atgtatgtgt gaagataaaa ttgtaaatat aatttcagac ctagtagggg
    39661 aaaatatgtc tgttagaaaa ataggatgtc actgtttagg aggtaaatgc aaggcaacat
    39721 tcgttaaatc tcaaaaatat aaggctgtta cagaagtttc agcaatgtca agggatgggc
    39781 atatcctttg tggagacaat tacacataca tggagataaa tgatggtaaa tacatgatgg
    39841 ctataagtga tggtatggga aaaggtaaaa aagcatatga agaatcttct gccactattg
    39901 atatactgga aaaaatgata gatgcaaaaa ttaaagatga gatagtcatt gatactataa
    39961 ataatatgtt gttgttaaag tcttcagaag agatgttttc aactctggat ttgggaattt
    40021 tagatttaaa acgaggttgt ttagaaacta taaagatggg agcgtgttct acttatataa
    40081 aaagagagga tggagaagtt gacttaatat cgtcatcatc attacctgtg ggtatattat
    40141 cagatgtaaa aattgataga aaaaatgtaa aagttaagga aggagactat gttataatgg
    40201 tttctgatgg aattgtagat gcaggaagaa ataacaattt aggagacaat tggttaatat
    40261 acttcttaaa gaatatagag actacaaatc caaaggaaat ttcaaattta atattggata
    40321 gagcattaga gttacaagcg ttacaaattg aagatgacat gactgtttta gttacgaaaa
    40381 tatgtacaaa ataaggttta caagattatg tgtataagat gtggataact tgtgtataat
    40441 atgttaatag acttttgaaa aaagtatagt tattaacaaa aataacatat aaatcaacaa
    40501 aatttgttga taagtatggc agaaatgtta ataaaatgta aaaaaagaaa aaacaaaagc
    40561 tattatcaaa agagattcat gttgaaataa ctaaatttct aagtaaagaa aacctgtgaa
    40621 tatgttgatt attatgtgga taactattaa ttcgatattt atttatatag tatgaataat
    40681 tgaataatat atctaaaaaa agccatccta ttattaagta aacttattga aaggatggct
    40741 ttttatgaat agaaaattgt gcaaagcaat aataccttta tttatttcta ttttttgcat
    40801 gttaataata agtataaaag ctgaccaagg atatcaaaag aataatgata gagcaattac
    40861 tgtcatgaat aataatgata ctgaagatga tgctgaaaaa aaacaagaaa tagactatga
    40921 aaaaacatgt tggaatttgc aatctttgtt taaaagtgat gaccaatgga aaaaagaact
    40981 taagagtttt gataaagata ctaaagagtt gaaaaattac ataggtaaag taacaaaatc
    41041 gccaacacat ttgtcttttg ccttaaacat taaagaaaaa cttgatataa gactaaataa
    41101 actttctgcc tatgtaaaat taaaacaaga tactaataaa aattcgtata agtacttaga
    41161 tatgaatgat agtatgagta aatcctatgg gaattatatg gggatttgtt cagatttaga
    41221 gttagaaata ctaaaattat caaacaaaga ttacaagaaa attatttcaa ataaaaatat
    41281 aaataggaaa tatggtgcat atttaagaga tataagaagg aataaagtac attatttgga
    41341 tgataaatca gaagatattc taagcaaagt atcttcaata agtagtcttc caagccaagt
    41401 atatgaatta tttagaaata tggatagaaa aactaatttg acaccagctc aatatgcgag
    41461 tgaattagag tcatcagata gagaaaatag aaaaaaagct tatcaagatg aacttataac
    41521 ttacaatgat aatataaata caattgcagg tttaattact ggtcaagtta aaaagaatat
    41581 tttttattct caggaaagag gttatgagtc ttctttagaa atgtacctgg aatctgataa
    41641 tgtagatgaa aaagtataca ataatttgat aaggactgta aatagtaata tggacagtct
    41701 acacaaatat atagatttaa gaaaaaaagt attaaaatta gataaggttc attcatatga
    41761 tatgtttgtg cctatggtta aggctgttga taataatatt aattatgaaa aggcacaaag
    41821 tatagtatat tcagcgttgt cacctttagg aaaagaatac aatgatgtag tatataaagc
    41881 tttcaatgaa aaatggatag atgtttattc acatgaaaat aaagtaagtg gtggatactg
    41941 cttatctgtt tatgaaaatc atccatatat acttttaaat tataataatt cattaggttc
    42001 agtttcgact ttatcacatg agcttggaca tgctgtatat gaatatttaa gttctaagaa
    42061 tcagaattat tttaattcaa atccgtctat atttactcac gaagttgcat ctacaacgaa
    42121 tgaagcatta ctttacgaga tgcttataaa agaagctaaa ggcaatgatg aaaaagctta
    42181 ttatataaca cagtatttag acttgataaa agatactctt tatacacaaa ctatgtatgc
    42241 tgaatttgaa aaaactatac acgagttagt agaaaaagga aaaagtgtaa acgcattagt
    42301 attaaatgat gtttggggac aacttcttaa aaaatattat ggacaaagct atgaattaga
    42361 ccaattatct aaggtcggat gggctagaat cccacatttt tataatagtt tctatgtcta
    42421 taaatatgct acaggttgta gtgcaggagt aagttttgcc caagacatcc taaagaatgg
    42481 ttcagataac tatatgagct ttcttaaaaa gggaagttca gattatccaa taaacttgtt
    42541 aaaatcaagt ggcataaatt taactaatac aaaacccata gaaaatacaa tcaaaaaatt
    42601 tgatagttta gtagcagaac ttgaaaagct attataaaca ttattgtaat tcacatggta
    42661 aatatatcga attaatgata aaatatacta aaagtttaaa gtcctatcaa gataggactt
    42721 tttatttatt aattagtggg agatgattct atgcaagtta aagttaaaaa ggctgtaata
    42781 ccagcagcag gtttaggaac tagatttttg ccagctacaa aggcacaacc aaaagagatg
    42841 ttaccaatag ttgataagcc aactctacaa tacataatag aagaagctgt ggcctcagga
    42901 atagaggaaa tactaataat aacaggtaga aataaaaaat ctattgaaga ccactttgat
    42961 aaatctgtag aattggaact tgatttagag aaaaaaggta agaaagaatt gttggaggta
    43021 gtacaaaaca tatctaacat gattaacata cattatataa gacaaaaaga gccaaaaggg
    43081 ttgggagatg caatttattg tgcgaggcat tttataggtg atgagccgtt tgcagtaatg
    43141 ttaggggatg atattgtaga taatgatgta ccttgtctaa aacaactaac agatgcatat
    43201 gaggaatata gaactacaat attgggtgtt caaaaggtaa atcaagaaga tactaataaa
    43261 tatgggataa tagaagcaaa aaatatagaa ggtagagttt acaaggtaaa agacatggta
    43321 gaaaagccag agtcaggaaa agctccttca aatattgcta ttttaggtag atatataata
    43381 actcctgaaa tctttgatat tcttaaggat ttaccaccag gtaaaggtgg agaggtacaa
    43441 cttacagatg ctttaaaaat attatctaag aaagaagcta tgtatgcata caattttgaa
    43501 ggcaagagat atgatgttgg ggacaagcta ggatttttag aagctactgt tgactttgct
    43561 ctaaagaaag aagaccttaa ggaagatttt ataaaatatt tgaaacatgt atgtagtgaa
    43621 tttgataaaa ataacaatgt gttatacaat aatacagatg aaaaaataga tttaattgaa
    43681 gtcaatgaag ctagagaact tgaaattcaa aaataattaa tgaaaggtga tgtatggtgg
    43741 caaaatcaag tcagaaaaga attaatagaa taatagctgt tgggattgtg ggaatcctcg
    43801 taataacgag tataacagct gttttgagta tgattgcaac tatgtaataa gataaatgac
    43861 agcagttgat atacataaat ataaaaacca tatataactt gatgtagtca aaaacttaca
    43921 atattttagt tatatatggt ttttagaatt tataaatgta aaaaaaaatt tttataatag
    43981 ttgcatattt taaaggaaaa tattattata agatataaat atagtgataa atttataaaa
    44041 acgtgctaag attggacttg aaatacagta atatagtagg taataatgtt ttaaatttta
    44101 tttattgacc ataatatata aataatgata ttattagact taactgttta aataaaactt
    44161 ttctttttca tgccttcaat ttccaaaata agtatgtata taaatgaata tatagatgag
    44221 gaggattttt ttgattttag atgataaagt attagaagga ttaggtagta agaccttagt
    44281 tgagctacga gagatagcta aagaattaaa aataaaatca attactacat ataagaaaaa
    44341 tgagttaatt gagattataa attccaaaag taaaaatgag gaaaaagtaa acgaagagac
    44401 taaagaaaaa aatgaagata ttaaagaaga cataaagaat agaaaagaaa atatagagcg
    44461 tgaaagtata gaaaaagaag ttgaatataa gagccctacc aaatcataca acaagaaaga
    44521 aatagatacc caaaatattt cccaaaacca agaacataga aatcaaagaa atgactataa
    44581 taagacaacg agtgtaaatg atagtaataa gtcatcaaat atatctaata gaatggtcag
    44641 aaataataat aaaaactatt atatgcctaa acaagttgat gaatctaaga tagtagatga
    44701 atttaacaca tcaaaagagg atgaagtagt aggtgtactt gaaattctgc cagatggatt
    44761 tggtttttta agaggttcaa attatttgtc tactgaagga gatgtttacg tttcaccatc
    44821 tcaaataaga agatttaata tgaaaacagg agacaagatt aagggaataa ctagacatcc
    44881 aaaaagtgga gagaaattta gagcactttt atatgttcaa aaaataaatg atgaaaaccc
    44941 tgacacagct atacaaagaa atgcgtttga gacattaaca ccaatatttc cagaagaacg
    45001 acttacactg gaaactaata gaaatgaaat agctacaaga attatagatt taatttcacc
    45061 tataggaaaa ggacaaagag gattgatagt agcacctcca aaagctggga aaacagttct
    45121 tttaaaaagc gttgcaaata gcatagctaa aaatcatcca aatgttgaac ttatagtact
    45181 ccttatagat gaaagaccag aggaagttac tgatatgaaa gaatctattg aaggagacgt
    45241 tatatattcc acttttgacc aagtatcaag tcatcatgtc aaggttgcag agatggtgtt
    45301 aaatagagct caaagattag ttgaacatgg taaagatgta gtaattttac tagatagtat
    45361 aaccagattg gcaagggcat ataaccttac aatatctcct acaggtagaa ctttatctgg
    45421 aggtattgac cctggtgcac ttcatggacc taaaaagttc tttggtgcag caaggaatat
    45481 tagacagggt ggttctttga caatattagg aacagcattg gttgaaactg gttctaggat
    45541 ggatgatgta atatttgagg aatttaaagg tacaggaaat atggagctac atttagatag
    45601 aaaattagca gaaaaaagaa tattccctgc aatagatatt tacaagtctg gtacaagaag
    45661 agatgattta ttgcttgatg atgaagaaaa gactgctctt tggagattaa gaagagaaat
    45721 gagtaataat tcagtaatgg aaattacaga taaagtcatt gaacttataa agagaactaa
    45781 ggacaataaa gagtttgtaa aatcaataaa gaatttgtaa aatcaataat acaaatgcaa
    45841 atatggatta tataaactaa tagaaaaatc acttgaatgg cataataagt tgtgctataa
    45901 taatgtagtt gataatgtaa aaagaaagtt aatataatta atttttttaa ataaagagaa
    45961 gaggtgacta taatgcaaaa ggaaatacaa ccaaaataca atccagttga agtgcgttgt
    46021 gcttgtggga acacatttgt tgctggttca actaaggacg aaataaaagt tgaaatatgt
    46081 tcagagtgcc atccattcta tacaggaaaa caaaagaaca tagaaaaagg tggaagaatc
    46141 gacaaattca agaaaagatt caaaatggac taatttttat aaaagtggag ttggaggaag
    46201 ccaactccta ttttattaca aaaaaacatt catattctac tttaaattgt ctttttgtat
    46261 tataataata aatatgtaaa attgagtatt ctaaatacaa taaaatgagg tgagtggatg
    46321 gcaaaacaag ctgtaggtgg gcaagctgtt attgaaggtg taatgatgca gtcaaaagac
    46381 aaaagagcgg tagctgttag aaaaagtgac ggagaaatag cactaaaaga agatagaata
    46441 aaaagttggg taagagataa gaatatagat aagatacctt ttgttagagg ttcttttgtt
    46501 atgatagata caatgattca aggcataaaa agtttaaact tttcttcaga gttttttatg
    46561 gaagaagctg aagaggataa atttgattta tttataaaga aaatttttaa agataaggca
    46621 aatgatataa ttataatatt ttcattggtc atagcaatgt tactttctgc tggattattc
    46681 atttttatac ctacattggt aggaggagct ttttctaaag taatgcctaa tgattttatg
    46741 cttaatttaa tagaaggaat cataaggata gctatattat ttgcatatat agtattgatt
    46801 tcacgaagta aggatataga aagagtcttt caatatcatg gagcagaaca taaatctata
    46861 tattgctatg aaaatgattt agaacttaca gtagagaatg ctagaaaatt taaaagatta
    46921 catcctagat gtggtacaaa tttcctattt atagttatgg ctgtatcaat aatattgttt
    46981 gcattttttg gttggccaaa tccaattctt agaatattta tgagaattat atgtgtgcct
    47041 atagtagcag gtttatctta tgaggtaatt agagttcttg ggaaatatga taatggattt
    47101 actaaaatta ttgcatatcc aggaatgatg ttgcaatatt ttacaacaaa agagcctgat
    47161 gatgagcaat tagaggttgc tttggaagca ttgaaagcag tagtagatta gagaaaagga
    47221 tatttgttat gacgataaaa gatataatta ttaaatattc agataaacta aaagatataa
    47281 gtgatactcc aagattagat acagagttat tgttacaaaa aacattgggt gttgatagat
    47341 tatatattca cttaaatctg aataaagagc taactgaaga acaaaaaaca aaatttatgg
    47401 gttttgcaga agaaagatta aatggaagac caatagcata tatagttgaa aatagggagt
    47461 ttatgggatt agattttttt gttaaggaag gcgtattgat tccaagacca gatacagaaa
    47521 ctttagtaga agaaataatt gaaatatgta gagaaaaaaa agatgtaagt atattagata
    47581 taggaactgg ttctggagct attacaataa gtttagcaaa gtacattgaa aattctaaaa
    47641 tcatgtcttt tgatatatca gagacagcat tagaaatagc taaaaaaaat gctattataa
    47701 atgaagttgg tgaaaaaata aagtatatta attctgattt atttacagca ataagtgata
    47761 gtaacataaa attcgatatc atagtttcaa atccaccata tattaaaaaa caagatatag
    47821 aaactctgca caaacaagtt aaagattatg aaccatataa tgctcttgaa ggtggagaag
    47881 atggattaga cttttataga agaataacag aacaaggtaa gaaatattta aataagtgtg
    47941 gaatattagc atatgaagtt ggtcataatc aagctgaaga tgtaataaat attatgaaaa
    48001 gtaatggata taaaaaaata tacacaaaga aggatattca aggcattgat agagttgtta
    48061 taggttacaa tatatgatat aatcaacaat tgatatgtta catttaatcg aggtgaaagt
    48121 atgttaaaaa aattagaggt attggaagat acatataaag acttaagtga aaaaataggt
    48181 gatccggatg taataaatga tcaaaaagtt tggcagaaat atataaaaga acatgctgac
    48241 ttggaaccta ttgttatgaa atacagagag tataaaagcg ttttagatag tataaaagaa
    48301 tcaaaagaaa ttttacaaga agagtcagat gaagaattaa gagaattagc taaaatggag
    48361 ttagctgaga tggaagaaaa agtagcacct ctggaggaag aaataaaaat actattattg
    48421 cctaaagacc ctaatgatga taagaacgtt atagttgaaa ttagaggtgg agctggagga
    48481 gatgaagcag ctctatttgc tggtgattta tttagaatgt actcaaggta tgctgaaaga
    48541 agaagatgga aaatagaatt attgagtgca agtgatactg gagttggtgg atataaagaa
    48601 gtatctttta tgataaaagg taaaggtgct tattcaagat taaagtatga atctggagtt
    48661 catagagttc aaagaatacc atcaactgaa tctggtggaa gaatacacac atcaacttct
    48721 actgttgcag tattaccaga agttgaagat gtagaggttg aaataaatcc aaatgattta
    48781 agaatagatg ttttccgttc ttcaggtaat ggaggacaaa gtgtcaatac tactgactct
    48841 gctgttaggg taacacatat acctacaggt gaagtagtat cttgtcaaga tggaaaatca
    48901 caattaaaaa ataaagagca agctttaaaa atattaaaag ctagacttta cgataaagca
    48961 ttggcagaac aacacaaaga tatagcagca gaaagaaaaa gccaagtcgg aacaggagac
    49021 aggtcagaaa gaataagaac atataatttc cctcaaggta gaataagtga ccatagaata
    49081 aatttaactt tatataagtt agatgcattc ttagatggag atatagatga aatgatagat
    49141 gctttaatta ctgttgacca aacagaaaaa atgactgcaa tataagtaat gttttaaaga
    49201 agctgtttct tcctagaaat tattttctat tttgaaacag ctattttgca ttttaggtca
    49261 tgttatcata ttctattgta tataattgtt atatacaagt ataggagggt tatatgatac
    49321 taaaagtaac aataattggg cttttggcag gtgttatagg gacaggatta ggaggagtaa
    49381 tatcagccat ttttaaaaga gaggtcgata aatatctaag tttttttatg ggattatctg
    49441 gtggaataat gttggctgta gtggtgtttg atttaatgaa agaatccatg gacaaaatgg
    49501 ggataatcaa tacagttata tttacgtttg ttggagctct cataactatg tacataaaaa
    49561 caaaattaga tgtgtctgga aatatggcat ctggatatct tatattcata agtatattac
    49621 tacataattt accagaagga ctagccattg gttcttcttt tatgtcaact gaaagtttag
    49681 gtattacact tgctatagtt ataggtctac ataatatacc agaagggtta gctatggcat
    49741 taggcttagt ttgcaataaa atgaaactaa gtaaagttat tttgtttact gtaatagctg
    49801 gattacctat gggtctagga agttttttag gtgtttattt tggaggagta tttacttctc
    49861 ttataggagt atttcttgct acagcaggag gtactatgat gtatgttgta ttggaagaaa
    49921 tttttccaca ttcaaaaagt gtgtattgta taattggttt cttgttaggt acaatgattg
    49981 taaattatat ataaaattaa aatgaaagca ggcatacaaa agatgaaaac tattataagt
    50041 aatattgata taaataatat tgattatgag gaaataaaaa tacaagcaaa acttcttaga
    50101 gaaggtaaaa cggttatttt tccaactgag acagtatatg ggttgggtgc aaatgcatta
    50161 gatgaaaatg cagtaaagaa gatttatgaa gctaaaggaa gacctagtga taatccattg
    50221 atagtccata tatatgaaaa agaagaagta tatgacttag ccaaagatat aagtgataaa
    50281 gctaaacttg ttatagaaaa gctttggcca ggacctataa caattatatt aaataaaaaa
    50341 gatataattc catacaaaac tagtggaggg cttgatacag ttgcaataag gatgccttct
    50401 aatgttattg ccagggcaat aataaaagaa gctggtatac ctattgcagc tccttctgca
    50461 aatatctcag gtcgcccatc tccaacaaaa gcaaaacatg tatatgaaga gatgaatgga
    50521 agagtggatg gaatagtttt aggaggagac agtaattttg gattggagtc aactgtattg
    50581 gatttaacag aagagactcc tatgatactg agaccaggaa gtataactaa agaagtttta
    50641 gaggaacttc ttggagaagt aaagcttgac ccatcattaa gtaaaaaaga agataatcaa
    50701 aaagctaaag ctcctggaat gaaatataaa cattattctc caaatgcaga tgtatatata
    50761 atatctggaa aaagagaaaa tgtagcaaca aagattaatg atatgataaa atctaataga
    50821 gacaaaggtc taaagacagg tgtaatgtgt attagtagaa accgagagtt ttatgatgga
    50881 gaagtaatcg atttaggaaa ctctcttgaa gaagttgcaa gtaatctgtt tgatgctctt
    50941 atagaaatgg acaaaaaggg tgttgatgtc atatattcag aagagttccc caagactgga
    51001 gttggacaag caattatgaa tagactactg aagtcagcag gatacaaagt gataaaggct
    51061 taagagggag ttgtaaaata tgaatatact tattgtgtgt acaggtaata cttgtagaag
    51121 ccctatggct gaggctatac taaggaaagc aattaaagaa tctggaagaa gtattgaaga
    51181 atattctata tcatcagcag gtatatctac agcaaatgga atgggtgctt ctgaaaattc
    51241 tatagaagta ctaaaagaaa tcggtataga cttatcaaat catagaagta aagtaattac
    51301 taaaaaatta attgatgaat cagatataat actaacaatg actaaatctc ataaagaaat
    51361 tttagtacaa gcagttccaa aatgcaaaga aaaagtgtat acttttaaag ggtttgcaaa
    51421 taaaaatgag gaagatatat ctgacccatt tggtggaaat ttggatattt atagaagcac
    51481 tatgagagaa ataatgtatt ctgtcaacga aatagtaaaa aaaatatgaa acggaggata
    51541 aacaaatgaa gataggatta ggctgtgacc atggaggata taatttaaaa aaagaaataa
    51601 taagctattt agaaagtaaa ggtatagagt gtgtagatta tggaactaat aatgctacag
    51661 attctgttga ttatcctgtt tatggagaaa tagttgcaaa ttcagttata aataaagaag
    51721 tagattatgg aatattatgt tgtggtacag ggataggaat atctttagct gctaataagg
    51781 ttcctgggat aagatgtgca gttgtatcag atgtattttc tgcaaaaatg tcaaaagcac
    51841 ataatgatgc aaatatgtta tcattaggcg aaagagtttt aggaaaaggt ctagcacttg
    51901 agatagttga agcatggatt aatacggatt ttgaaggtga tagacatgct agaagagtaa
    51961 acatgattaa atcaatagaa gaaaaacata ataaataata aaaattacag ttttatcagg
    52021 aggaattatc aaaatgagca aagtggtaga gacaaaccat ccattaatac aacataaatt
    52081 aactttaatg agagataaaa atactggttc taaagacttt agagagcttt taactgaaat
    52141 tgcaatgtta atggggtatg aaataacaaa agatatacca ttaaaagatg tagaaataga
    52201 aactcctatt caaaaaacat catctaaagt agttgcaggt aaaaaattag caataattcc
    52261 aattttaaga gcaggtcttg gaatggtaga tggattagtg agcttaatgc cagcagcaaa
    52321 agttggacat gtaggactat atagagaccc agaaacatta aaaccagttg aatattattg
    52381 taagcttcct caagacatag gagaaagaga tataatagta gttgacccta tgcttgcaac
    52441 agggggttct gctgtagcag ctatagattt acttaagtct aaaggagcta aaagtataaa
    52501 attagctaat ttagtagctg caccagaagg tatagcagaa gtgcaaaaat atcatgatga
    52561 tgttgatata tatgttgcat cagttgacga aagattaaat gaacatggat atataattcc
    52621 tggtcttggt gatgctggag atagattgtt tggaactaaa taaaaataaa taactataaa
    52681 tatttattag ggactttctt atatagtgta tataatttta tgtaagaagg ttctttttta
    52741 tatatttttt ctcaaaatag gaattattga attgataaat ttctcgaatt gagaataaaa
    52801 agtttttacc aattaaaaaa gattgcttat tttacaaggt tttatagaat agtagtctat
    52861 tttaatgtgg catgttagtt gcttacgatt ataggtatta agtgtattag ttaaattgag
    52921 aattgaatat taataatatt attgggagtg gtaatacatg aagaattgta tagtagtaag
    52981 aggggctgga gatttagcaa cagcagttat tcataaatta cacaacagtg gttttaaagt
    53041 agttgcgtta gaaatagaag agccattagc tataagaagg caaatttctt tttgtgaggc
    53101 tgtttatgaa aaaaaagtaa ctgttgaagg tgtagaatgt atattttgca atactgaaaa
    53161 agaaatagag gaagttttat ctcaacataa agtagccttg attatagacc caaaaggaga
    53221 aaaattatct aaattaaatc caatcgtttt ggttgatgga atactcgcta agaaaaactt
    53281 aggtacaact aaagatatgg caccacttac aatagcttta ggtcctggat tttgtgcagg
    53341 agaagatgta gatgttgtta tagagacaat gagaggtcat aatctaggaa gaataataac
    53401 taatggtaga gcagaaaaaa atacaggagt accaggagca ataaaaggtt tttctaaaga
    53461 gagagttatg cattcacagt atgatggcat aatggaaaat gtatgtaaga taggtgatat
    53521 agttgaaaag ggagacctac tttgctatat taataattat gaaaaaaaac atgaagttag
    53581 agcaacaata tctggagtcc ttagaggttt attaaaggat ggagcaaagg taaacaaaaa
    53641 tttaaaaata ctagacatag accctagaaa agaagaagtt aagaatgctt ttacaatatc
    53701 agataaagct agatgtatag ctggaggggt tttagaagcc atactaaaca atgggattta
    53761 tccccaaaat taatttatat tagataaagc tattaatatt ttattaagat gatataataa
    53821 atttaataga ttaaagagag catatgacgg gtatttaaaa tttaaatggt aaaaatgctc
    53881 tttttttatt ggacattcaa ttaacatgtg tatataatat aaaaataagt atttatttag
    53941 gttgaaatct aataaatata taataccagt acataagcaa agaggagaaa agtaaattat
    54001 gagaccatca tgggatgaat acttcatgga aattgcagaa gtagtgaaaa agcgttcaac
    54061 ttgtattaga agacaagtag gagctgtaat tgtaagagac aaacaaatat taacaactgg
    54121 atataatggt tctccgagaa atcttgaaca ctgtgaaaat attggatgta agaggcagga
    54181 attaaatatt ccctcaggag aaaggcatga attatgtaga gcattacatg ctgaacaaaa
    54241 tgcgataata caagctgctc ataatggtat tagtgtagat ggggcaactt tgtatgttac
    54301 tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat gcaggtatat tgaaaattgt
    54361 ttatgaaggt gattatccag atgatatgtc tacagaatta ttaaaagaag caggaataga
    54421 actcataaaa ttttagtgga taaaatttat atgacaataa atgtcaaatt atgacactga
    54481 aatacaatgg tatcaaatga ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa
    54541 gatatatttt ataattaaat aaatttatga gatagattaa ttacatgtta tttattttat
    54601 aataatgagg gatactattt atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt
    54661 ttagtttaat aagtcaaata ggattgatga ttgtaatatc aatattagga tgtactttct
    54721 taggaaaatt tttagatagt aaattgaata ctactccagt tctgacaata atatttttat
    54781 tgttgggtgt tggaggtgct tttagtggtg tttataaaac tctaatagta tatacacgaa
    54841 ggaagtgata tattggatat aaaattactt aaccaggtaa ggtcagtaac aaaaggtata
    54901 gtaatatttg atgtagtttt aattgtgcta atgttgataa catcaacatt aagtaaacca
    54961 gtattattag gacttatatt tggctctata atagctatgt taaattttag attattggcg
    55021 atatcactgg aaaaatcagt gacatttcct gctgggaagg cacaagctta tgcatcagca
    55081 caatatgctg ttagattatt tataatggca gttgttctaa ttgcatcagc tacagtacca
    55141 catttaaata taatcggaac agcattggga ttattgagca caaagtttgt catattatca
    55201 aaaaacttta taaataaatt aaagaggaag gaggcataag tatgaatcaa gcgcattatt
    55261 atctcatagg aaactttaag attagtgaaa caatactagt aagttgggga ataatggcct
    55321 ttttagcgat agcatcttat tttttgacta gaaacttaaa aaaagttcca actagtaaag
    55381 ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa aatggttaca gatgttatgg
    55441 gtaaaaatac agtaaaaaga atgccatata tgataccata cataggaagc ttgtttttat
    55501 ttttcgcatg ttcaaattta agcccattac ttggattaag gtcaccaaca accgacttag
    55561 acacaactgc tgcatgggca atgattacat tttttatgat ttattttgca ggagttaaat
    55621 ttaaggggtt atcatacttt aaagaattgg ctcaaccact tgccataatg ttaccaatca
    55681 atattatagg tgagttagct agaccaatat cattaagttt ccgtccattt gggaatattt
    55741 taggtggaac aataataatg ggtctgtttt atcaactttt aggttttata tctagtttaa
    55801 taccaggatt atcaatacca attggagaaa tattaatacc agtcccatta cacttgtact
    55861 ttgacttatt tgcaggattg ttacaagcat atatatttat aatgttaaca atggtattta
    55921 tatcaagtgc aggagagtta gattaagaaa tcaacttata ttaagataaa aataaaataa
    55981 tttaaaaaat taaagtttta aggaggaaat aattatggaa acagcaatag tagcggcagc
    56041 atcagcaata ggagcaggga tagcagttgc aacaggtata ggtgcaggta taggtcaagg
    56101 tatagcagca gctaaagctg cagaagcagt aggaaaccaa ccagaagcta aaggtgatat
    56161 aacatcaact ttattactag gagttgctat agcagagtct tcagcaatat atggtttggt
    56221 tatatcaata atacttctat tcgtaaatcc attctttaaa tacttaggaa tgtagattta
    56281 gtccttaaat attttgagat aggtttatag gtaaatcatc tcaaacttat aatcatgaga
    56341 tgaactttat catctcgtga ttataagtag ttatgattta tggaaaggag gataattatg
    56401 gaaaaagcac tagtaggtat aacgtgggaa tttgtgttcc aaattgttaa tacttttata
    56461 atatttttac ttctgagaaa gcttttattc aagccagtat taaatataat tgaatcaaga
    56521 gaaaatgata ttaaaagtga tttggcagaa ggcgaaaaag caaaaaatga aggtttagct
    56581 ctgaaaaaag aatatgagtc aaaaatcaat tttgccaagg atgaaggtca agaaatcata
    56641 aaacaagcaa ctataagagc agagcagaaa tcagatgata tagtaaatac tgcaaagaaa
    56701 gatgctcttg atataaaaga aaaagcaaac aaagatatag aacaagaaag acaaaaggtt
    56761 ataaatgaaa tcaaaaatga tatatctaac atagcactac ttgcagcatc taaagtcatt
    56821 gaaaaagatt tagataaatc taaacatgaa gaattgatag aaaactttat aaaagaggta
    56881 ggggaagcta aatgataaat gtaatagcaa atagatatgc agaagcccta tttcaattag
    56941 gagaagaaga aaattcaact gatgtcttat tcaaagaact agaaaaagta gtagatatga
    57001 tgactaaagt tagtaaagat ttctacaaag ttttaaaatc accattagtt tcaaaatcag
    57061 aaaagaaaaa cttggtagaa attattttta gtaaagaagt aagttctaac ataaaaaact
    57121 ttttaaaagt tttagtagat aaagatagaa tatcatattt agaagatatc gaattagctt
    57181 ataaagaatt attaaataag aaaaacaatg taattgatgg agtagcaata agtgctattc
    57241 caatgagtga aacagatatt aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa
    57301 atgttactat agaaaatgtt gttgacaaga ctatactagg gggagttcta gttagaattg
    57361 gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat
    57421 ctgaagtaat atcttaagaa tggcggtgaa cccatgaact taaaacctga agaaataagt
    57481 tctataatta aacagcaaat aaaaaattat gagaataaag ttgagttgac agatacaggt
    57541 agtgttttaa cagttgggga tggtatagcg agtgtatatg gattagaaaa agctatgtct
    57601 ggtgagttgc tagagttccc aggtgaaata tatggtatgg cacttaactt ggaagaagaa
    57661 gtagttggag ctgttatcct tggagatgac agtgaaataa aagaaggcga tatagttaag
    57721 agaactggaa gaatagttga agttccagtt ggagaagctt taataggtag agttgtaaac
    57781 tcactaggtc agccaataga tggaaaagga cctatagcgt acacaaaaac tagaccagtt
    57841 gaatctgagg cacctggtat aatagataga aggtctgttt atgaaccatt acaaacagga
    57901 atcaaatcaa tagattcaat gataccaatt ggtagaggtc aaagagagtt aataataggt
    57961 gacagacaaa ctggtaaaac atctatagtt attgacacta tactaaatca aaaaggaaaa
    58021 gatgttatct gtatatatgt agcaattgga caaaaacgtt ctactatagc tcaattagtt
    58081 agctctttag aaaaaggtgg agctttggat tatacaatag tagtttcagc aacagcatct
    58141 gaatcagcac cacttcaata tatagcacca tatgctggag cagctatggg tgaagaattt
    58201 atgtacaatg ggaaacatgt acttatagtg tatgatgatt taagtaaaca agctgttgca
    58261 tatagagaaa tgtcattgtt acttagaaga ccaccaggac gtgaggcata tcctggagat
    58321 gtattctatt tacattcaag attacttgaa agagcagcta agttatctga tgaattgggt
    58381 ggaggttcaa tgactgcctt gcctataata gaaactcaag ctggtgacgt ttctgcatat
    58441 ataccaacaa atgttatatc tataacagat ggtcaaatat acttacaacc agagttattc
    58501 tattcaggtg taagaccagc agttgaccct ggtatatcag tatcaagggt tggtggttct
    58561 gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa aacttgcata ttcacaatat
    58621 agagaacttg cagcattctc acagtttggt tctgacttag atgaagatac taaaaagaga
    58681 cttgctcaag gtgaaagaat cgttgagata ttaaaacaag gtgagcatca accaataaaa
    58741 gttgaaaatc aagttatgat aatatatgct gttataaata atcatttaga ggatattccg
    58801 atagataata tagctagatt tgaatcagaa ttgtatgcat ttgtagataa taattatccg
    58861 gaaatatcaa gaaaaatatt aggtggagaa gacttcactc atgatttaac agatgcaata
    58921 aatgaattta aagaaaagtt tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat
    58981 taattggcag gagctggaat caaagcaatc aaaacacgta tgggaactgt tgaaagtaca
    59041 aagcaaataa ctaaagcaat ggaattagtt gcatcttcta agcttagaaa agcaaaagaa
    59101 aaggctgaag attcaagaga atattttaat actttataca atacagtaaa agatattgcc
    59161 caaacaacac gtggaattag aaatgaattt ttaaaagaaa gagaagtaaa aaataaatgc
    59221 tatgtagtag tagctggaga cagaggactt gcaggtggat acaattctaa tgtattaaag
    59281 gaatctgtct ctcatatgaa aggtaaaaag gaaacagtga ttactgttgg aaagaaagca
    59341 catgaatttt tttctaaaag aaactacaat atagcaaaag atataaatag tgttgaagct
    59401 tgtggatatg aagatgcgac agaaatttca caaactatta ttgacctgta taaaaaaggt
    59461 gaagtagatg aagtttatat tgcttataca aaatttaaat ctgctttatc acaagaagtt
    59521 gaaattttga aattgcttcc attagtcttt gaaaaagata cagacgaaca aggaatggca
    59581 gtaaaagaag aaaaacaaaa tagatcaaaa gtacaatata taccatcagc agaagcagtt
    59641 ttaagttata ttgtacctca gtttatatca ggtacagtat atggtggaat tttggaatct
    59701 tttgcatcag agcaaggtgc tagaagaact gcaatggaat cagccacaga caatgcgaat
    59761 gaaatgctat ctaatttaga gttaagttat aacagagcaa gacagtcagc tgtaactcaa
    59821 gaaataacag agatagttgg tggagttgaa gctctaaaat agggaggtta ggaaatggca
    59881 aatgtaggta aagtagtaca gattgtagga gctgtactag atgttaagtt tgatagtgaa
    59941 caaagcctac ctaacttatt aaatgcgtta gtaataaagt taggagataa agaaatagtt
    60001 gcagaagttg ctcaacatat aggtgatgat actgtaagat gtatagctat gagtgcaaca
    60061 gacggacttg taagaggtat ggaagttgta gatacaggag ggccaataag tgttcctgtt
    60121 ggagatgaaa ctttaggtag aatattcaat gttcttggaa aaccagttga tgggaaacca
    60181 gctccaaaat ctgcacctaa attacctata catagaccag caccagcata tgatgaacta
    60241 gaaactacag cagagatact agaaactggt attaaggtag ttgacttact agctccatat
    60301 ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag taggaaagac agttctaata
    60361 caagagctta taaacaatat tgctaagcaa catggtggta tttctgtgtt ttcaggagta
    60421 ggagaaagaa caagagaagg taacgacctt tatggcgaaa tgagtgagtc tggagttata
    60481 aataaaacag ctctagtatt tggtcaaatg aatgaaccac ctggagcgag aatgagagtt
    60541 gctttaactg gacttacaat ggcagaacat tttagagatg agcaaggaca agacgtttta
    60601 cttttcgttg ataatatatt ccgtttcaca caagctggtt cagaagtttc agcacttcta
    60661 ggacgtatgc catcagctgt tggttatcag ccaacattag ctactgaaat gggtgcactt
    60721 caagagagaa taacatcaac taagaaaggt tcaataacat ctgttcaagc agtatatgta
    60781 ccagcagatg acttaactga cccagcacca gctacaacat tctctcactt agatgcaaaa
    60841 acagttttat caagacaaat atcatcactg ggtatatatc cagcagttga ccctcttgaa
    60901 tctacatcaa ggattcttga ccctagtata gtaggaaaag aacattatga agtagctaga
    60961 ggtgtacaat caatacttca aagatataaa gaacttcaag atataattgc tatacttggt
    61021 atggatgaat tatctgatga agataagtta atagttgcac gtgctagaaa gatacaaaga
    61081 ttcttatctc aatcattcac agttgctgag caatttacag gaaatccagg tcaatatgtt
    61141 cctgttaaag agactgtaag aggatttaaa gaaattcttg aagggaaaca tgatgactta
    61201 ccagagtcag cattcttatt tgtaggaaca atagaagacg ctgttagaaa agcgaaggga
    61261 agtatgtagt catggccagt gaattttcat tgaaagttgt aactccaagt aatacttttt
    61321 atgatggaga tgttgaaatg atagtagtta gaactacaaa aggagacaaa ggggtgttga
    61381 aaggtcatat accatttgtt tcaggtatac ctgtaggaac tttaaagata aaaaaagagg
    61441 gaagttttaa agaggcgaaa atagcagaag gattcattaa tgtaactcaa gataaaactg
    61501 tcatattgac agaatctgct gagtggattt aataaattaa tttagagtat aaaaagtcat
    61561 tttgaccaaa aaaacttttg ggtaaaatgg cttttttgcg ttttggtgtt aataaaataa
    61621 cgataaaatt gtaagttttt agtgataata aattcacaaa atgtttaaat ttactgccaa
    61681 attatgttgt aattaataag ataaagaaga tttagatagc ttttatgtaa gtgaaaaagt
    61741 cttcatataa gaaatacata taaaattttt ataaaattta aaaaatttgc ttagttgtgt
    61801 agtataataa ataatataat agatttgtat ataatattca ttacaaataa ttttaaaagt
    61861 tacagtttta ttataatgat tattaaatta agataagtaa cataaactaa ataataatgt
    61921 gtattatcga tagtaaattt ataaattcat tattaattat tgaaagaagt attataaatt
    61981 ttctggagga acaatgaata tatttgatat tggcgtagat ataattgaaa ttgatagaat
    62041 aagaaaggct gtagacaaaa ataataggtt tttggagaag atatttacag atagagaaat
    62101 agagtatttt aattctaaga attttaaagc agaaagtata gcaggtaatt tcgcagccaa
    62161 ggaagctata agtaaatcta taggtacagg aattagattg tttaatttta aagatataga
    62221 agttctcaga aatgaaatgg gaaaaccaat agtaaaaact tataataatc tagcaaaaat
    62281 gtgcatagat tataatgttt tagaaataaa agtttcaata tcacattcaa aagattatgc
    62341 tatagcaaat gcgataacca taattaagga ctaaggagtg attacattat gatggataaa
    62401 acagcaaaag aaataatgac aacagatgta atagtagcaa aacaagatga tagtatagca
    62461 gatgtagcta atatgctaat tgcagaaaaa ataggaggtc tgccagttgt agattcagag
    62521 aatagagttg ttggaataat ttctgaaaca gatattctta aaaaagaaaa atatatagaa
    62581 gcacctctct atataaatct attacaagga ttgattttct tagatgactt aaaaaaagtt
    62641 gaaaaagata taaaacaagt agctgcttat aaggttggag aacttatgtc taaagatata
    62701 ataaaggttc atgaagatga taagtttgat gatgtagcta atgtaatgat taaaaaatca
    62761 ataaacagag ttcctgtagt agatgatgac aacaaactta aaggtataat ttgtagatat
    62821 gatataataa aagctttata caatgaataa actgaagtag gaggtgtgat gtgagaaaaa
    62881 agtggaccat agtatgtatt atgttcttgg cactggttat aattgtaata ggatgccaaa
    62941 aacgacagtc cacaaaagaa gaagtataca aagattttca aaagcagatt tctgatatga
    63001 attattacag ttgtaaagct gaagtagaag tagttggaaa taaaagccca cacaattatg
    63061 ttttaattca tacttataaa aaaaccgata actataaatt agaagtaatt tccccaaaac
    63121 atttaaaagg gaaaagtata gaatatcaag atgataaaat attagttaaa aatccaaaga
    63181 ttagtgatgt agtggagtta cctaatacag gaaaaaataa tcaatatctt tttgtaggag
    63241 actttataaa aaactatctt caaaatgagg agatgaaagt aaagttatcc aaaggacatt
    63301 tggttttaga gacttttatt cctggggata ataaatattt taacaagcaa gtattatatg
    63361 taaatgctga cacaaaaaat cctgaaaaaa tggaagtgtt agataaagag ggagtgccaa
    63421 gatttacagt aaaatacaaa gattttgaat acagaaacta agggggacta aagacatgca
    63481 aaaaataaca gtgcctacat gggcagagat aaatctagat aacttaagat ttaacttaaa
    63541 taatattaaa aatttattag aagaagatat taagatttgt ggagtaataa aagctgatgc
    63601 atatggacat ggtgcagtag aagttgcaaa attgctagaa aaagaaaaag tagattactt
    63661 agcagtagca agaactgctg aaggaattga acttagacaa aatggcataa cacttcctat
    63721 tttgaacttg ggatatactc cagacgaagc ttttgaagat tctataaaaa ataaaataac
    63781 tatgacagtt tattctttag aaacagcaca aaagataaat gaaattgcaa aatctttagg
    63841 agaaaaagcc tgtgttcatg ttaaaataga ctcagggatg actagaatag gtttccaacc
    63901 taatgagaag tcagtacagg aaataataga attaaataaa ttagaatata tagatttaga
    63961 aggtatgttt actcattttg ctacagctga tgaagtaagt aaagagtaca cttataaaca
    64021 agctaataat tataaattta tgtctgataa attagatgag gctggtgtaa aaatagctat
    64081 aaaacatgta tcaaacagtg cagctattat ggattgccct gatttaagat taaatatggt
    64141 aagagcagga ataatattat atggtcatta tccatctgat gatgtattta aagatagatt
    64201 agaattaaga ccagccatga aattaaaatc aaaaatcgga catataaaac aagttgaacc
    64261 aggtgtagga ataagttatg gactaaaata cacaactaca ggtaaagaaa caatagctac
    64321 agttccaata ggatacgcag atggatttac tagaatccaa aaaaatccaa aggttcttat
    64381 taagggagaa gtgtttgatg tagttggtag aatatgtatg gatcaaataa tggttagaat
    64441 tgacaaagat atagacataa aagttggaga tgaggttata ctatttggag aaggcgaagt
    64501 tacagctgag cgtatagcta aagacttagg aactataaac tatgaagtgt tatgtatgat
    64561 atcaagaaga gttgaccgtg tttatatgga aaataatgag cttgtacaaa taaacagtta
    64621 tttgctaaaa taaaaccatg atattataat ctgggaggcg attttgtgta cgataagagt
    64681 aaagaaaaaa tagaagttac tttacctgac tctctgattt ctgaagttga tagtatagtt
    64741 cagatggaga acagtaacag agaagatttt gcaaaagctg cttttcagtt ctatataact
    64801 cagaaaaaga aaattcatat taaagaatct atgaaaacgg gttatagaga aatgggtcaa
    64861 attaatctgt cactagctga attaggtatg acagttgaag aagtatcttc tgatgataaa
    64921 aatgaaggga agattgccca aggtgagtac taattgtgaa ataaagcgtg gtgatttata
    64981 ttacgctgat ttaagtccag ttgttggttc agagcaggga ggcgttagac ctgttctgat
    65041 aatacaaaat gatgtaggta ataaatatag tcctacagtt atagtatcag ctataacatc
    65101 tcagattaat aaagccaaat tacctactca tgtagagata agttccaatg aatatgggct
    65161 taataaagac tcagtaattc ttttagagca aattagaact atcgataaaa agagactgag
    65221 ggaaaaaatt ggttgtctag atgaaaatat gatgataaaa gtagatgatg gacttcaaat
    65281 aagtttagga ttgtttactt tttaaagagg gatttgtctt ttattagaca atcccttttt
    65341 tcttttaaat atctcttaag gtttaatttt aatgtactat gttaataaaa ttaaaaataa
    65401 cgcaaaaaaa tagcataaaa gtgatataat aatatggaat tgatataaaa ttacgaactc
    65461 aagggaggta attgcatgag agaccataaa ggattaaatg agattgcgcg tatcataaga
    65521 agagatatag tttcaatgat acatagagcc aaatctggac atccaggagg gtcattatca
    65581 gtagttgaaa tacttactgc gttatacttt gatgaaatga atgtggattc ttcaaatcct
    65641 aaaatggagg atagagatag atttgtatta tcaaaaggac atgctgcacc agcattatat
    65701 gctacattgg ctgaaaaagg ctattttgat aaggaagaac tgaatggatt aaggaagata
    65761 ggaaggatgt tacaaggtca tccagacatg aaaggcacgc caggtgtgga aatatctact
    65821 ggttctttag gtcaaggttt ttcagttgca tgtggtatgg ctatggcatc aaagttggat
    65881 aatgctccgt ggaatgtgta tactttgctt ggtgatggtg aggttcaaga aggaattgtt
    65941 tgggaagctg ctatgagtgc tgcacattat aaacttgata atttaattgc ttttttagat
    66001 aataatggac ttcaaataga tggagatata gaaagcgtaa tgagtttagg ttcaatagtt
    66061 gataaattta aagcttttgg ttggaatgta attgagatag atggacatga ttttgaccaa
    66121 atatttgcgg ctcttgatat agctaaatca acagttaaaa aacctacaat gatagttgca
    66181 aaaactataa aaggcaaagg aatatctttt atggaaaatc aagcaggatg gcatgggaca
    66241 gctccaagtg atgaggagct agaaaaagct ttgcttgaat taggaggtgc tgacaatgag
    66301 taaaatggcg acgagagaag catatggtaa agcattagta aaattaggtc aaataaatga
    66361 caatgttgta gtattagatg cagatttatc aaagtctaca aaaacacatg atttttacaa
    66421 gtcatttcca gatagatttt ttaatatggg tatagctgaa caaaatttaa taggcgctgc
    66481 ttgtggatta tcaactgctg gtaaaatacc ttttgcaagt acttttgcta tgtttgctac
    66541 gggaagggct tttgagatta taagaaattc agtttgttat ccaaagctaa atgtaaaaat
    66601 atgtgctact catgcaggtc ttactgttgg agaggatgga gcatctcatg aaagtgtgga
    66661 agatattgct ataatgagag ctataccaaa tatgacagtt cttgttccag ctgatggagt
    66721 tgaaacagaa aaaattattt ttgaaatagc taagtataat ggacctgttt atgttagatt
    66781 agggagaagt tcagtaccag ttttatttga tgaagattat aaatttgaaa taggaaaagg
    66841 tacagtctta agagaaggaa aggatgtatc aattatagct tgtggaatta tggtaaatga
    66901 agctctatta gcacaagaaa agttgcaaga agaaggtata agtgctagag ttataaacat
    66961 gtcctcgata aaacctatag acaaagattt aatattagaa tcagcaaaag aaacgaatgc
    67021 tattgtaact gtagaagaac atagcataat tgggggatta ggttctgctg taagtgaggt
    67081 agtaggagag tcttgtccaa ccattgtaaa gaaagttgga attaaagata catttggaga
    67141 atctggtact ccaaatgaat tgttaaaaaa atatgaatta acttgtgatg acataataaa
    67201 gactgttaaa gaagttatta ttgcaaaaag aatgtaagag atataaatta ttcatgtttg
    67261 ttaataatat aatatattta aaagagaggt ataaagtgtt atattaattt atatctctct
//
LOCUS       DAYASV010000005        64361 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYASV010000005 DAYASV010000000
VERSION     DAYASV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.005"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(359..871)
                     /locus_tag="ACGI68_000351"
     CDS             complement(359..871)
                     /locus_tag="ACGI68_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0136809.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            1321..1797
                     /gene="purE"
                     /locus_tag="ACGI68_000352"
     CDS             1321..1797
                     /gene="purE"
                     /locus_tag="ACGI68_000352"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFH0136810.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDNNLRG"
     gene            1803..2501
                     /gene="purC"
                     /locus_tag="ACGI68_000353"
     CDS             1803..2501
                     /gene="purC"
                     /locus_tag="ACGI68_000353"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006772094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFH0136811.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            2513..3880
                     /gene="purF"
                     /locus_tag="ACGI68_000354"
     CDS             2513..3880
                     /gene="purF"
                     /locus_tag="ACGI68_000354"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFH0136812.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            3874..4950
                     /gene="purM"
                     /locus_tag="ACGI68_000355"
     CDS             3874..4950
                     /gene="purM"
                     /locus_tag="ACGI68_000355"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFH0136813.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMERYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAVAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNADYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            4944..5537
                     /gene="purN"
                     /locus_tag="ACGI68_000356"
     CDS             4944..5537
                     /gene="purN"
                     /locus_tag="ACGI68_000356"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFH0136814.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            5530..7062
                     /gene="purH"
                     /locus_tag="ACGI68_000357"
     CDS             5530..7062
                     /gene="purH"
                     /locus_tag="ACGI68_000357"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFH0136815.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            7073..8323
                     /gene="purD"
                     /locus_tag="ACGI68_000358"
     CDS             7073..8323
                     /gene="purD"
                     /locus_tag="ACGI68_000358"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFH0136816.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTTVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            8348..12154
                     /locus_tag="ACGI68_000359"
     CDS             8348..12154
                     /locus_tag="ACGI68_000359"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFH0136817.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLKTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDDKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVLGSTVKETSIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI68_000360"
     CDS             12360..13232
                     /gene="rfbD"
                     /locus_tag="ACGI68_000360"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFH0136818.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIKIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI68_000361"
     CDS             13341..14210
                     /gene="rfbA"
                     /locus_tag="ACGI68_000361"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFH0136819.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI68_000362"
     CDS             14259..14816
                     /gene="rfbC"
                     /locus_tag="ACGI68_000362"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFH0136820.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI68_000363"
     CDS             14854..15837
                     /gene="rfbB"
                     /locus_tag="ACGI68_000363"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFH0136821.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            15867..16535
                     /locus_tag="ACGI68_000364"
     CDS             15867..16535
                     /locus_tag="ACGI68_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFH0136822.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVNQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            16544..16972
                     /locus_tag="ACGI68_000365"
     CDS             16544..16972
                     /locus_tag="ACGI68_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HFH0136823.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            16996..17310
                     /locus_tag="ACGI68_000366"
     CDS             16996..17310
                     /locus_tag="ACGI68_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0136824.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVKVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     VN"
     gene            17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI68_000367"
     CDS             17461..17736
                     /gene="flgM"
                     /locus_tag="ACGI68_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFH0136825.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            17741..18157
                     /locus_tag="ACGI68_000368"
     CDS             17741..18157
                     /locus_tag="ACGI68_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HFH0136826.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGNDISMSEVVENSNDGYLKEIYTDLKRILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI68_000369"
     CDS             18175..19485
                     /gene="flgK"
                     /locus_tag="ACGI68_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFH0136827.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI68_000370"
     CDS             19504..20436
                     /gene="flgL"
                     /locus_tag="ACGI68_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFH0136828.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI68_000371"
     CDS             20452..20844
                     /gene="fliW"
                     /locus_tag="ACGI68_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HFH0136829.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDNGC"
     gene            20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI68_000372"
     CDS             20838..21050
                     /gene="csrA"
                     /locus_tag="ACGI68_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFH0136830.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI68_000373"
     CDS             21072..21467
                     /gene="fliS"
                     /locus_tag="ACGI68_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFH0136831.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQKAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQVI
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI68_000374"
     CDS             21467..21823
                     /gene="fliS"
                     /locus_tag="ACGI68_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFH0136832.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQNAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI68_000375"
     CDS             21844..23367
                     /gene="fliD"
                     /locus_tag="ACGI68_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFH0136833.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSINSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVRQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            23370..23705
                     /locus_tag="ACGI68_000376"
     CDS             23370..23705
                     /locus_tag="ACGI68_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFH0136834.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            23807..24679
                     /locus_tag="ACGI68_000377"
     CDS             23807..24679
                     /locus_tag="ACGI68_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFH0136835.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            24772..26913
                     /locus_tag="ACGI68_000378"
     CDS             24772..26913
                     /locus_tag="ACGI68_000378"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0136836.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDIENEDTIYNIEYLKSLR"
     gene            26937..28814
                     /locus_tag="ACGI68_000379"
     CDS             26937..28814
                     /locus_tag="ACGI68_000379"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0136837.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            28807..30921
                     /locus_tag="ACGI68_000380"
     CDS             28807..30921
                     /locus_tag="ACGI68_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0136838.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     ASQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYMESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIKRYNTSEFDRDRIKEKQSEIKHFVIDKHCIEIKNYIDKIYNEANHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            30930..31925
                     /locus_tag="ACGI68_000381"
     CDS             30930..31925
                     /locus_tag="ACGI68_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HFH0136839.1"
                     /translation="MNNIHINKFEKSCERNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLREQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            32017..33249
                     /locus_tag="ACGI68_000382"
     CDS             32017..33249
                     /locus_tag="ACGI68_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFH0136840.1"
                     /translation="MKRLMILGSIKYFENIVISAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAIMKKYFKNNAIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLLYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIELYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            33269..34213
                     /locus_tag="ACGI68_000383"
     CDS             33269..34213
                     /locus_tag="ACGI68_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HFH0136841.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ELFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            34286..34714
                     /locus_tag="ACGI68_000384"
     CDS             34286..34714
                     /locus_tag="ACGI68_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFH0136842.1"
                     /translation="MNRIDKFMDKYNLIRFVSIGNEYIGNLVALESNKVIPFDIKRVY
                     YIYDVPNNVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGV
                     WHEMFDFDEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            34792..35892
                     /locus_tag="ACGI68_000385"
     CDS             34792..35892
                     /locus_tag="ACGI68_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFH0136843.1"
                     /translation="MNLLLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHF
                     EEEFANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGAT
                     PVFVEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDC
                     AQSHGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGS
                     EKRYYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKI
                     ENNCTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNI
                     AENYSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      36201..36291
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            36700..37017
                     /gene="flgB"
                     /locus_tag="ACGI68_000386"
     CDS             36700..37017
                     /gene="flgB"
                     /locus_tag="ACGI68_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFH0136844.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            37025..37429
                     /gene="flgC"
                     /locus_tag="ACGI68_000387"
     CDS             37025..37429
                     /gene="flgC"
                     /locus_tag="ACGI68_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFH0136845.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            37439..37756
                     /gene="fliE"
                     /locus_tag="ACGI68_000388"
     CDS             37439..37756
                     /gene="fliE"
                     /locus_tag="ACGI68_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFH0136846.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            37781..39331
                     /gene="fliF"
                     /locus_tag="ACGI68_000389"
     CDS             37781..39331
                     /gene="fliF"
                     /locus_tag="ACGI68_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFH0136847.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            39344..40414
                     /gene="fliG"
                     /locus_tag="ACGI68_000390"
     CDS             39344..40414
                     /gene="fliG"
                     /locus_tag="ACGI68_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFH0136848.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            40398..41138
                     /locus_tag="ACGI68_000391"
     CDS             40398..41138
                     /locus_tag="ACGI68_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HFH0136849.1"
                     /translation="MTLLNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFR
                     DEVIKEAIESAEIRKNEILEEAKMNAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYT
                     QKAKDEADFIKNQADLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESM
                     SDLLKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGN
                     AIIQKENGRIIVGIDCAMEKLKEELLGE"
     gene            41142..42461
                     /gene="fliI"
                     /locus_tag="ACGI68_000392"
     CDS             41142..42461
                     /gene="fliI"
                     /locus_tag="ACGI68_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFH0136850.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRIFE"
     gene            42493..42939
                     /gene="fliJ"
                     /locus_tag="ACGI68_000393"
     CDS             42493..42939
                     /gene="fliJ"
                     /locus_tag="ACGI68_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFH0136851.1"
                     /translation="MELDKNFKFRLQKVLDLKIKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDNVQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEEQLKEQSTNDEFASMSYLRNRQVL"
     gene            42967..44172
                     /locus_tag="ACGI68_000394"
     CDS             42967..44172
                     /locus_tag="ACGI68_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFH0136852.1"
                     /translation="MKIQADSYNIIKDIGNRKNSSKNKNIKNNKFEDNLYKTTDHKNV
                     KDRRETSKQNVSSDRPGNSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     ENYKKDKDINTILNNIKKLSISDELKQELKLNKNILNKIANQNLELNGANTNNPNDIE
                     NLDIMKLRELLENNASEDIQVISKTTENDTKSIDLYSFDSNRMDTLNSNKQKDDSSDI
                     LEKLAGLNGNKSGNFGQVINKSVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSNQNTNDKRNNVVIEDEIKENII
                     DDDRVDLLA"
     gene            44190..44798
                     /locus_tag="ACGI68_000395"
     CDS             44190..44798
                     /locus_tag="ACGI68_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFH0136853.1"
                     /translation="MYGIATALAGTQVNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMND
                     KLDILLALSNSTLINSALSMATSLIGKEVEVSGEEDEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVDVKYEKLLKIGEVEANSGEENDSSEQESPEEV"
     gene            44825..45808
                     /locus_tag="ACGI68_000396"
     CDS             44825..45808
                     /locus_tag="ACGI68_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFH0136854.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMTQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            45821..46024
                     /locus_tag="ACGI68_000397"
     CDS             45821..46024
                     /locus_tag="ACGI68_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HFH0136855.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            46024..46842
                     /locus_tag="ACGI68_000398"
     CDS             46024..46842
                     /locus_tag="ACGI68_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HFH0136856.1"
                     /translation="MKKTDSLTFIGLILSTVLVLFGAVKGSSSGLKSFFDFASILITV
                     FGSFGALMITFTIDDIKLIKDALQYSFKTMNISKLDLLNQFKTLSKKARREGLLSIES
                     DVMKIQDPFMKKGLELCIDGLEINEIRSILELEIDSVEETNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLSDLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            46832..47527
                     /locus_tag="ACGI68_000399"
     CDS             46832..47527
                     /locus_tag="ACGI68_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFH0136857.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSMFSTGSSTSEKSPIESVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKEDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPKLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            47539..47982
                     /gene="fliL"
                     /locus_tag="ACGI68_000400"
     CDS             47539..47982
                     /gene="fliL"
                     /locus_tag="ACGI68_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFH0136858.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQIDKIAGLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            47997..48371
                     /locus_tag="ACGI68_000401"
     CDS             47997..48371
                     /locus_tag="ACGI68_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HFH0136859.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYIKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILRIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            48372..49037
                     /gene="fliP"
                     /locus_tag="ACGI68_000402"
     CDS             48372..49037
                     /gene="fliP"
                     /locus_tag="ACGI68_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFH0136860.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLKQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            49050..49319
                     /gene="fliQ"
                     /locus_tag="ACGI68_000403"
     CDS             49050..49319
                     /gene="fliQ"
                     /locus_tag="ACGI68_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFH0136861.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMMSLITK"
     gene            49331..51145
                     /locus_tag="ACGI68_000404"
     CDS             49331..51145
                     /locus_tag="ACGI68_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HFH0136862.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGINETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLINNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKSFIDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIIGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSTDKTEEATPKKKGEQRKKGNIAKSK
                     ELPIAMTLLAFTLLVPTLFSYVVNTLKSSLNYFLSLDFYMNINYSSIEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIVANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIVIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEIKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNDIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            51154..53229
                     /gene="flhA"
                     /locus_tag="ACGI68_000405"
     CDS             51154..53229
                     /gene="flhA"
                     /locus_tag="ACGI68_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFH0136863.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGGLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDEDKAIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMNNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            53207..54220
                     /gene="flhF"
                     /locus_tag="ACGI68_000406"
     CDS             53207..54220
                     /gene="flhF"
                     /locus_tag="ACGI68_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HFH0136864.1"
                     /translation="MTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIRGIFSK
                     KDIELTIGWEKKDNLEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLNLDLNQ
                     EIVEFIKADLQEMKFNGIDTSKNLVESLKKKIKIENQAINGKIALVGPPGVGKTTTIA
                     KLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEMK
                     DCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRNV
                     NFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLGV
                     ESI"
     gene            54217..55095
                     /locus_tag="ACGI68_000407"
     CDS             54217..55095
                     /locus_tag="ACGI68_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HFH0136865.1"
                     /translation="MIDQAQILRKMAMEKRGSDEIIVENENMPKIITIASGKGGVGKS
                     NLATNLSICLTKLDKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHNVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKKSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVGVCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            55109..55810
                     /locus_tag="ACGI68_000408"
     CDS             55109..55810
                     /locus_tag="ACGI68_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0136866.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDTEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            55826..56248
                     /locus_tag="ACGI68_000409"
     CDS             55826..56248
                     /locus_tag="ACGI68_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFH0136867.1"
                     /translation="MIQIILIVVSILIIVTIVINLQKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            56233..56418
                     /locus_tag="ACGI68_000410"
     CDS             56233..56418
                     /locus_tag="ACGI68_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HFH0136868.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            56459..57232
                     /locus_tag="ACGI68_000411"
     CDS             56459..57232
                     /locus_tag="ACGI68_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFH0136869.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            57253..58014
                     /locus_tag="ACGI68_000412"
     CDS             57253..58014
                     /locus_tag="ACGI68_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFH0136870.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGKDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            58037..58918
                     /locus_tag="ACGI68_000413"
     CDS             58037..58918
                     /locus_tag="ACGI68_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0136871.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNECIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            58921..59295
                     /locus_tag="ACGI68_000414"
     CDS             58921..59295
                     /locus_tag="ACGI68_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFH0136872.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            59327..60730
                     /locus_tag="ACGI68_000415"
     CDS             59327..60730
                     /locus_tag="ACGI68_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0136873.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLDMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYNSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            60846..62783
                     /gene="htpG"
                     /locus_tag="ACGI68_000416"
     CDS             60846..62783
                     /gene="htpG"
                     /locus_tag="ACGI68_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFH0136874.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            63171..64301
                     /locus_tag="ACGI68_000417"
     CDS             63171..64301
                     /locus_tag="ACGI68_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0136875.1"
                     /translation="MNFNYNLPVNILFGSGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        26499 a         6612 c        11774 g        19476 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaata aaatacttta atgaaaaata gctaacttta attgtaggat attcttaata
      181 tatttgaaat aaataaaaat attgaaggat ataatattca actggtatat aaataataaa
      241 tgatagttaa attttacctt agaggctaat taggtgttta gtcaaagcta atgcctttag
      301 aatacaaatg tattctaaag gcatttttac tatgtatttt catttatatt actaaacatt
      361 atctattttt atatacattt ctatgtattt tattttcatc aaatcggtct ataaactcat
      421 ttttttcttc actatatcca atagatataa gtgcataagg aactaatttt aatggtaagt
      481 ctaaagtttg tctaacttta tttaccactt tatttttagg aaatactcct gcccaacaag
      541 cagctagacc ttgattagta acttctaata ataaattttg tgtgcaagca cctaaatctt
      601 gtatccattt accaggtttt aagaatttat ctctatttcc aagcactact atacataatg
      661 gtgcaaattc tatatgcttt gctctatgct gtgactttga gagctgtttc agcagttcct
      721 tatcatctac tataataaat tcccaaggtt gagcattttt tgatgaaggt gcttgcatag
      781 ctgattttag tatttcagtt attttatctt cttctatatt tttatctata tatgttctta
      841 ttgaacgtct attttttata acttctaaca tacagattct cctttaaata aaattatttc
      901 tatttatata atatcattac tttaaatttt agctattact tatataaaga ataaactttg
      961 tgttggtttt atctaagtaa aagtaacatt cagaactaaa aaacaagact ataaatccaa
     1021 agtattaatt aaaaaagaag taataaaaaa taatatacaa gatttaaaaa agagtattgg
     1081 gcttaaaatt taattagaaa accaattttg agataatctg ttttttattt taacaaaaaa
     1141 acataaataa agttcgatac gtagcgaatg aaaaaattac aagaaatatt ttttttaaaa
     1201 aatttcaaaa aagagttgat aatatatgta tattaatata aaatttaagg tgtataattc
     1261 gtataataat acgaatgatt tgtagtgtaa aataacttat aaacggagag gggaagtcta
     1321 atgaaagtag cagtagttat gggttcaaaa tcagattatc caaaattaga agaagggata
     1381 aaactgcttg aaaaatatgg aatagaagtt gtagcaagag cattatcagc tcatagaaca
     1441 ccagaacaac tttcaatatt cctaaaagaa atagaagatg atactgatgt aataatagca
     1501 gcagcaggaa aagcagcaca cttaccaggt gtaatagctt cacaaacatt aattcctgta
     1561 ataggattac ctattaaatc atcaactatg gatggactag actcactttt atcaatagtt
     1621 caaatgccaa aaggaatccc agttgcaaca gttacaatag acttaggttt aaacgcagct
     1681 ttattggcat tacaaataat gactttaaaa tacccaaaat taaaagagga tttgaaatca
     1741 tatagagaag agatggcaca aaaggtgtta gaagacgaca ataatttaag ggggtagcag
     1801 aaatgttatt atatgaagga aaagcaaaac aagtatattc tacagataat gaaaatgaat
     1861 atgttgttta ttataaagat gatgcaacag catttaatgg agaaaaaaaa gcagaaatat
     1921 cttcaaaagg tatactaaat aataagatat caacaataat ctttgaaatg ttaaaagaaa
     1981 ataatataaa tactcacttt ataaaaagtt tatcagatag agaaatgtta gttaaaaaag
     2041 tagagatttt accattagaa gtaatagtaa gaaatattgc tgctggttct atctgtaaaa
     2101 gagttggtct tgaagaaggt gtagttttcg atgagccaat ttttgaaata agctacaaaa
     2161 atgatgctta tggtgaccca atgctaaatg atgattatgc agtggcaatg aagttagcaa
     2221 ctagagaaga gttaaaattc ttaagagaag aaactttaaa aataaatgag ttactaaaag
     2281 catttttctt aaaattaaac ctaaagttag ttgactttaa aatagaattt ggtaaggatt
     2341 ctgaaggaaa tataatattg gcagatgaag tatcaccaga cacttgtaga ctttgggatg
     2401 taaatacaaa tgaaaagtta gataaggaca gatttagaaa agaccttgga gaccttgtag
     2461 aaggttatac ggaagtactt tcaagaatga ataataaata ggaggcaaat gtatgtgtgg
     2521 agttttaggg atttactcta ataaagatgt aactaaagaa ctatattatt ctttatattc
     2581 tatgcaacac agggggcaag aaagctgtgg tttagctctt ttagatgatg gagaaattaa
     2641 gtacaaaaaa gatatgggat tagttggaga tgtttttaag gaaaatgaat tatctaagtt
     2701 aaaaggaaat atagggattg gacatgttag atattcaaca gctggaggaa gtcatgtttc
     2761 taattgtcaa cctttagttg gaagttgtag aaaaagacaa ttagccatag ctcataatgg
     2821 aaatctagtt aatgcaaatt atttaaaaga tatgctagaa gaagatggat atatgttcca
     2881 aactaattca gatacagaag tgattttata tatacttgct agatattaca aaggggatat
     2941 agtagaaagt ttaaaagtga caatggatta tataaaaggt gcatatgctc ttgtaataat
     3001 gagtcaagaa gaattggttg cagttagaga cccacatgga ttcagacctc ttgtacttgg
     3061 taaaaagggt gatgaatata tatttgcatc agaaaattgt gcaatagata tacttggcgg
     3121 agaagttata agagatgtag agccaggaga gataatagta gtcaaagatg gagaactaaa
     3181 atcatatttt tattcagaaa attataaacc agttaagaaa agttgtatat ttgaacacat
     3241 atactttgca agaaatgatg caactataga caatgtaaat gcatatgaat ttagaattaa
     3301 atgtggtgag agattagctc agaatgaaac tgtaaaggca gatatggtag ttccagttcc
     3361 agattcaggt tggccaggag ctattggtta tgcaaatgct agtggattaa aaataagtga
     3421 aggcttagtt aaaaatagat atgtaggaag aacatttata aaacctacac aagaagaaag
     3481 agaaatagca gtaaaaataa aattaaatcc tctttcaaca ataataaaag gaaaatctat
     3541 aattttagta gacgactcaa ttgtaagagg aactacatct aaacaattag taaaatcttt
     3601 aagagaagca ggtgcaaaag aaattcatct tagaataact tctcctccag tagcttactc
     3661 ttgttactat ggaatagata ctccaaatcg ctctaaatta attgcatcaa gtaacaacgt
     3721 agaagaaatg agagaatata ttggatgtga tagtttaaaa ttcctagata tagaaggaat
     3781 gttagatgca acagaacata agtctacttt ctgtaaagct tgttttgatg gagaataccc
     3841 agttaagaaa attgataagg aggaactact ttcatgttaa catataaaga atcaggtgta
     3901 gatatagatg aaggaaatag agcagtagac cttataaaag gtaaaataaa aggaacttat
     3961 gatggaaatg tagttggaga tttaggtaac ttcagtggat tatacagttt aaaagatttt
     4021 gtaggtatga aagaaccagt tttgcttgca tctacagatg gtgttgggac taagttaaaa
     4081 atagctcaga tgatggacaa gcatgatact gttggaatag atttagttgc aatgtgcgtg
     4141 aatgatttaa tttgtcaagg agcaaaacca ttattcttcc ttgactatat agctcttggg
     4201 aaattagtgc ctgagcatat agaaaaaata gttggtggaa ttgcagatgg ttgcaaaatg
     4261 tctggttgtg cactaattgg tggagaaact gctgaaatgc ctggaatgta tggagaagat
     4321 gactatgatt tagcaggatt ttctgtggga atagcagaca aagaaaagat tgtttctgga
     4381 aacaatgtta aatcaggaga tgtattggtt ggtatatctt caagtggagt acacagcaat
     4441 ggattttctt ttataagaaa gatattttta gaaacttaca attataaaat ggaacgatat
     4501 gttgaagaac taggaatgac agttggagaa gcacttttaa caccaactaa aatatatgtt
     4561 aaattagctt tagatgtatt agctaaacat gacataaaag ctgtagctca cataactggt
     4621 ggagggctta tagaaaatat aacaagagtt atacctaaag gattaggact agatatcaat
     4681 aaaaaatctt gggagaagcc tcctatattt aaaatgatag aaggttttaa tgctgttgat
     4741 gagagagaac ttcataagag ttttaacatg ggtattgggc tagttttaat agtagataaa
     4801 gaaaatgctg atgatgttgt aaactttata aataatagag aaaatgacaa tgctgattat
     4861 gtagataaaa aatatagtga attattagaa gataaagctt atataattgg tgaagtagta
     4921 gatagtcatg aaggtgtaga attatgctaa atataggagt tttaatatct ggtgggggaa
     4981 caaatttaca agcagttata gatggtactg agtctggaga aataaaaggt caggttaaag
     5041 tagttatctc aagtaagcaa ggtgcttatg ggctagagag agctaaaaat cataacataa
     5101 aagcaatttg tgaaactgat gaagacaaga taatagaaat tcttaaggaa aataaaatag
     5161 atttagtagt tttggctggt tatttaaaaa taatcagccc aaagctggtt aatgagttta
     5221 gaaacaaaat gataaacata catccatcat taattccttc attttgtgga gctggattct
     5281 atggagaaaa agttcatcaa ggtgttatag attacggagc taaagtaact ggtgctacag
     5341 ttcattttgt tgatgaaggt gctgatactg gaccaatcat aatgcaagat gtagttaagg
     5401 taaatcaaga tgatgatgca aagacacttg caaaaagagt gttagaagtt gaacatagga
     5461 ttttaaaaga aagtataagt cttttttgtg aaaataaatt aaaacttcaa ggtaggagag
     5521 tgtttataaa tgagtaagag agctttaata agtgtaactg acaagactgg tgtagtggaa
     5581 tttgctaaag agttaaataa attagattat gaaattatat ctacaggaaa tacatttaaa
     5641 acattaaaag aaaatggtgt taatgtaatg caagttgaag atgttacaaa ttttccagaa
     5701 atattagatg gaagagtaaa aacattaaat ccatatatac atggtggtat actttataaa
     5761 agagataaag aatctcatgt agaaactgta aatgaacata aaatacattc aatagattta
     5821 gtagctgtaa atctatatga ttttgaagga actttaaaag ctggaaaaag tcatgatgaa
     5881 ataattgaaa atatagatat tggtggacca tctatgatac gttctgctgc caaaaactat
     5941 aaggatgtaa tagtagtagt agatataaaa gattatgatt ctataataga aaaattaaaa
     6001 acagatacaa tgacattaga agacagaaaa aaattatcat ataaagcttt ctcaactaca
     6061 ggaagatatg atgcgttaat atctagttat tttgctggag aagttggaga tacgtatcct
     6121 gacatattaa atttaacttt ccaaaaagaa caaactttaa gatatggaga aaatccacat
     6181 caaaatggtt tcttatattc acagtcaaat gcaaaaaatc caatcttaaa ttatgagcaa
     6241 ttaggcggta aagaactttc ttttaacaac ttaaatgatt tacatggatg tttagaagta
     6301 atgagagaat ttaaagatag tgaagaagta gtttcagtag ctataaaaca tgctaattca
     6361 tgtggggtag gtttaggaaa agatgctttt gaagcatata caaaatgtta tgaagcggat
     6421 aaggtttcaa tatttggagg aatagtagga ataacttcta ctatagataa agctacagca
     6481 gagaaattaa atgaaatatt cttagaaata gtagtagcat atgattttga accagaagct
     6541 ttagagatac ttaaacaaaa gaaaaatcta agaatattaa aattagctaa aatagaaaat
     6601 agtttacaac catatgaaat gaaatattta gatggaaaat tattgataca agatagaaac
     6661 aatatattag cagaaaaaag tgaaaatgta acaaaggaaa aaccaacaga tgcacaactt
     6721 aaggacatgg aatttggtat gagagtagta aaaaatatga aatcaaatgc aattgcaata
     6781 gtaaaaaatg gacaaactct agctttaggt tgtggacaaa catcaagaat ttgggcatta
     6841 aaaaatgctc ttgaaaataa caaagataaa gattttacag gagctgtttt agcgtcagac
     6901 gcatttttcc catttgatga ttgtgtaaca ttagctcatg aatatggaat atcagcagta
     6961 gttcaaccag gtggctctat aaaagataaa gattcaatag aagcttgtga caagtatgat
     7021 atggttatgg tatttacagg aataagacac ttcaaacact aaggaggata ttatgaagat
     7081 attggttgta ggcggtggcg gaagagagca tgctatatgc tggaaactaa gcaaagaaaa
     7141 aaatgtagaa aaaatttatt gtgcaccagg taatgcagga attgcaaacg tagcagaatg
     7201 tgtaaatata ggtgatacta atatagaaga acttttaaaa tttgccaaag aaaatgaaat
     7261 aggtctgaca atagtaggac cagaagtacc actagttatg ggaatagtag atgagtttga
     7321 aaaagaagga ttaagagtat ttggacctaa taaaaaatgt gctcaacttg aaggaagtaa
     7381 agctttttca aaagaattta tgataaaaca taacatacct actgcaaaat ataaagaata
     7441 tacaaactta gaagaagcta tatcagaaat agatagtttt ggatatccag tagtaataaa
     7501 agcggacgga ttagctgctg gaaaaggtgt tgtaatacct gaaaatagag aagatgctat
     7561 agcaacttta aaagaaatga tgtcagataa gaagtttgga gctgctggag ataaaatagt
     7621 aattgaagag tttttaaaag gaatagaaac ttcaattcta gcatttgtag ataatgatac
     7681 aatagttcct atggcaagtg caaaagacca taaaaaagta aacaactatg agcaaggtcc
     7741 aaacactggg ggaatgggaa ctttttcacc tagtgaaata tatacagaag aattagcaaa
     7801 taaagtaaaa gagacagtat tagaaaaaac tttagaaggc ttcaaaaaag atggacttaa
     7861 ttttaaaggt attttatttg ttggtcttat gataactgaa gatggagaaa aagtacttga
     7921 atataatgta agatttggag accctgagac acaatctgtt ttatttagat tagaaacaga
     7981 tttacatgag ataatggaag ctatactaga taataagtta aaagatatag aaataaacta
     8041 tagtgatgat gaagctgtat gtgttatgct tacatcagga gggtatccag acagttatga
     8101 aaaagggaaa attataactg gtcttgaaaa cttggatgat gatatagtag tattccatag
     8161 tggcactaag atgtttgatg gtaatttggt taccaatgga ggaagagtaa ttggtataac
     8221 tgcaaagtca actacagtta aggatgcagc agaaaaagtt tatgaaaaca taaagaagat
     8281 aaactttgaa ggaatgcact atagaacaga cataggaaga taatttttct aaaatggagg
     8341 ataaggtatg ttgaatactg aaaataaaga ctcaatggtt agaagagtat tagttgaaaa
     8401 aagagaaggt tttgaccttg aggctaaagc tttaaaaaaa gacttagtag aaagtttaca
     8461 catagataat atagaaaatt taagaatatt aaatagatat gatgtagaag gaatatcaga
     8521 agaagtttat gaaaatgcag caaagacaat attctctgaa ccaaatttag atgtagtata
     8581 ttatgaagaa ataccaaaat taaatgatga aagagtattt gcaatagaat ttcttcctgg
     8641 tcaatacgac caaagaggtg attgggcagc tcaatgtgta caaattgtaa atcaagggat
     8701 aagacctgct attaatacag caaaagttta tatattatca ggcaaaataa ctgatgaaga
     8761 gttttctaaa ataaaagatt actgtataaa tccagtagat agtagagaag catctttaga
     8821 aaaaccagaa acgttaaaaa tggaaactga aataccaaca acagttgaag tattagatgg
     8881 atttatagat ttagatgaaa atggattaaa aacatttgta agtgaaaaag gattagcaat
     8941 gactttagga gacttacaac acgttcaaaa atactttaaa gatacagaaa agagaaatcc
     9001 aactataaca gaaataaaag tattagatac ttattggtct gatcattgta gacatactac
     9061 ttttatgact gaaatagaaa atgtaaagat agaagatggt aagtttaatg atatagtaaa
     9121 agaagcgtat caaatgtatc ttaactcaag agacaatgta tatgtgaaca gacataaaga
     9181 tatatgttta atggatatag ccactgttgc agtaaaagaa ttaaagaaaa atgggaaatt
     9241 aaatgaccta gatgaaagtg aagaaataaa tgcatgtagt ataaacgtag atgttgaagt
     9301 tgatggtaag atggaaaaat atctagtaat gtttaaaaat gaaactcaca accatccaac
     9361 tgaaatagaa ccatttggtg gagcagctac ttgtttagga ggagctataa gagacccatt
     9421 atcaggaaga agttatgttt atcaagcaat gcgtgttaca ggaagtgctg acccaagaac
     9481 gactttagaa gatactttac ctggaaaact tatgcaaaga aaaataacta cagaagctgc
     9541 tcatggatat agttcatatg gtaaccaaat aggtcttaca actggacaag ttgcagaagt
     9601 atatgatgaa aactttgttg caaagagaat ggaaataggt gcagttatag cagcagcacc
     9661 aaaagagaat gtagtaagag aaagacctga agcaggagat gtaatcgttc ttcttggagg
     9721 aaaaacagga agagatggct gtggaggagc aactggttca tctaaagaac atagtgaaga
     9781 gtcaatctta acttgtagtg cagaagttca aaaaggagat gctcctaacg aaagaaaaat
     9841 acaaagattc tttagaaata aagaagttgc tcaaatgata aaaagatgta atgattttgg
     9901 agcaggtgga gtttgtgttg ctataggaga gattgctgat agcttagata taaacttaga
     9961 cttagttcct aaaaaatatg atggacttga tggaactgag cttgcaattt cagagtcaca
    10021 agagcgtatg gcagtagcca taaaaaaaga aaataaagat aaatttatac aattagcagt
    10081 agaagaaaac ttagaagcta cgcatgtagc tacagtaaca gatacaggat atttaagaat
    10141 gttctggaat ggaaaagcaa ttgtagatat aaatagagag tttttagata caaacggagt
    10201 aaaacaaact actgatgttc atgttactaa agtagatgaa gaaaatacat tctttagctc
    10261 taatgaaata gtaaaagatg ttaagtgtgc aagtatgaaa gacaagttta caaaagtact
    10321 atctgacctt aatgtttgtt ctcaaaaagg attagtagag atgtttgata atacaattgg
    10381 tggtaataca gtacttatgc catttggagg aaagtatcaa gcgactccaa cacaaggtat
    10441 ggttgctaag ataccagtac ttggaggaga aactaatact tctacaataa tgacttatgg
    10501 atacaatcca aaggtaggaa aatggagccc attccatggt gccttgtatg cagtagtaga
    10561 atctgtgtgt aaattggttg ctattggagg aaattatagt acaacaagac ttacattcca
    10621 agagtatttt gaaaaacttg gtaacaatcc agaaaaatgg ggtaaaccat tctcagcact
    10681 tttaggagca ttttatgcac aaagtaaatt tgagatacct gctataggtg gaaaagacag
    10741 tatgtctggt acatttaaag atattgaagt tccaccaaca ttagtttcat ttgctgttga
    10801 cacagtagat gcgaagaaag tagtatctcc agaatttaaa aaagcagact caaaagtagt
    10861 aatgttatgt gtaaacaagg cagaaaatga tgttgtagat tttgaggaat taaagagaaa
    10921 cttagataaa gtaagagaac ttatacatgg aaataaagtt ttatcaacat atgcattagg
    10981 atttgcaggt gtaggtgaag caataagtaa aatggctttt ggtaataaaa taggatttaa
    11041 gtttagtgaa gaggctgaaa aagcatttac agatgataaa ttatttgagg ctagttatgg
    11101 aaatatagta cttgaattag caaatgatga tttaagtatg ttagaaggat acaattatgt
    11161 tgtgcttgga agtacagtta aagaaactag tatatttatc aaaggtgaag aacttgcatt
    11221 agatgaatta tataaagcac attgtagtac tttagagcct atattcccaa ctaaaacaga
    11281 agaagtaaaa tcaaagatag aaactataag ttatatatca caaggagaag ctaaaaaatc
    11341 atcattaagt atagctactc caagagtatt tataccagca ttccctggaa caaactgtga
    11401 atatgactct gcaagagcat ttgaaagagc aggagctaat gcaagtataa gagtatttaa
    11461 gaatttaact tataaagaca tagaagattc tatagataca atagtaaacg aaataaaatc
    11521 ttctcaaata ataatgttac caggtggatt tagtgctggt gatgagccag atggttctgg
    11581 taaatttata gctactgtat ttagaaatcc tagagtgcaa gaagctataa atgaattttt
    11641 aactcaaaaa gatggactaa tgttaggtat ctgtaatgga ttccaagtat taattaaatt
    11701 agggttagtt ccatatggag aaataagagt tccaagtgaa agtgctccaa cacttactta
    11761 caataatatt ggtcgccatc aagctaagat tgctagaact agaatttcat ctaataaatc
    11821 tccttggtta gctcaaacta atgttggaga tattcataat atagcaattt ctcatggaga
    11881 aggtaagttt gttgccagtg aagatgttat gagagaatta attgctaatg gacaagtagc
    11941 aactcaatat gttgacttta ataatgaagc tacttatgat attgagttta atccaaatgg
    12001 ttcattctat gctgttgaag gtataactag tgctgatggt agagtgtttg gtaaaatggg
    12061 acattctgaa agaattggtg aggaagtata taagaatatt ataggtgaaa aagaccaaaa
    12121 gatatttgaa tctggagtta aatattttag ataaagcttt gatgtttaat tgatagctag
    12181 aatatataat ttagatattt agatttaaag agatgtaata atatgatgat aatcatttaa
    12241 tattacatct cttttttatt tatctaagaa ataataagtt caacctaagt taaaatacaa
    12301 gctgtgttat aattaattat taagtaaaat tgaatattta ttggaggaca acataattaa
    12361 tgaaaatatt aataacaggg tctaatggtc aattaggaaa agaattggtt aaccaattag
    12421 aagcaatcaa tcaaagtata aaccaaccaa aatatgttat attagctaca acaagaagtg
    12481 atttggacat atccaatcag actaatgtag ataattttat tttgcataat aaacctgatg
    12541 tagttgtaaa ctgtgcagca tatacaaagg ttgatgcatg tgaagataat atcgaaattg
    12601 catacaaaat aaatgcttta ggagtgagaa atctagcaat agcatcagaa aaagttaatg
    12661 ctaagcttat tcacatatca actgactatg tattcaatgg tttttctaaa tatccatata
    12721 gggaagacaa taaaactgaa cccaattctg tatatggaaa aagtaaactt atgggagaaa
    12781 aatttgttga acaattctca cataagtact ttatattgag aacagcatgg ttatatggta
    12841 atgggaataa ttttgtaaag accatgataa aactttcttt agaaaataaa gaggttaatg
    12901 tggttaatga ccagtttgga actccaacaa gtacagtaga cttagcaaaa attataataa
    12961 aaatcatgga aacagaacat tatggagttt atcatgctac atgtgaaggt gagtgtagtt
    13021 ggtatgattt tgccaaaaag atatttaaac taaaaaatat agatataaaa gtaaatccaa
    13081 ttaagagtaa cgaatttaaa tgtaaggctc caagaccact atattctgta ttggataatt
    13141 ttatgttgaa gttaattggt ttaaattctt ttagaaagtg ggaagaatcc attgaagaat
    13201 atttagaaag atatgatata aatgaaaagt aaatgtatta ttttgagaaa agaaaataag
    13261 atattataaa aaatataaat atttctttta tttatccgat aatcataata tgacaaaaac
    13321 aaatttaagg atggaaaaag atgaaaggaa ttattttagc aggagggtct ggtacaagac
    13381 tatatcctat aacaaaatgt atatcaaaac aaacacttcc aatatacgat aaaccaatga
    13441 tatattaccc tatgtcggtt cttatgttgg cagggataca agatatatta ataatatcta
    13501 caccaagaga tataaaagca tttgaagaat tatttggaga tggaagttca ctagggctta
    13561 acataacata tgcaattcag gagaaagcaa atggaattgc agaagcgttt ataataggag
    13621 aagaatttat aggaaatgac agagtgtctc ttgtattagg ggataatata ttttatggat
    13681 atggatttag tgaaaggttg gagaatgcag ttaaaagaaa aggagcaact atatttgggt
    13741 atcatgtaag taatccaaat tcgtttggag ttgtagaatt tgataaggat tttaatgtat
    13801 tgtcaataga agaaaaacct acagttccta aatctaatta tgctatacca ggattatatt
    13861 tttatgataa tgatgttgta gagatagcaa agctagttga accgtcagat agaggtgaat
    13921 tagaaataac ctctataaac agtgaatatc taaaacgagg aaaactaaaa gtggaacttc
    13981 taggaagagg tatggcttgg cttgatactg gtacacataa aggtctattg gatgcttcta
    14041 attatgttga agtggttcaa acaagacagg gactgtatat agcatgttta gaagagattg
    14101 catatacaaa aggatatata agtagagaac aattaatgaa actagccaag ccactgataa
    14161 aaactgaata tggtcaatat ctattaaaat tggctagtga agatatgtaa tatggtagat
    14221 aaaatttatt ataaatttta ttaagggagt ttattataat gggaaaaatc aatgttataa
    14281 agactaaaat agaaggatta tatatagtcg aaccaaatgt attttatgat gaaagaggat
    14341 attttatgga aagctataat aaaaaggatt ttatagagca gggtcttgat atgtgtttta
    14401 ttcaagataa tgagtctagc tcaagaaaag gcgttttaag aggaatgcat tttcaggtta
    14461 aatatcctca aggaaaatta gtcagagtta cagagggaga agttttcgat gttgcagttg
    14521 atttaaggaa agattcacaa acatttggga aatgggaagg tgtaatattg tctgataaga
    14581 ataaaaaaca attttatatt ccagaagggt ttgcacatgg atttttagtt ttatcagaaa
    14641 aagcagtatt tagttacaaa tgtacaaatt attattaccc agaatttgag aatggcttta
    14701 tatggaatga caagcaaata tctattgagt ggcctttaaa aggaattgaa gaaatatttt
    14761 tatcagaaaa ggacaaaaaa caaaaaagtt ttaaagatat ttgtacaaga cgttaataaa
    14821 aatttattag aattaacatt taggagaaaa aatatgaaaa aaatactgat aactggagga
    14881 gcaggattta ttgggagtaa ttttatacat tatatgctta acaagtatgt agattatttt
    14941 attgttaacc tagacttgtt gacttatgct ggtaatttag agacattaat agaaatacaa
    15001 gacaattcca attataaatt tgttaaagga gatatttcaa atagggagca ggtgtataag
    15061 ctatttgaag aagaaaggtt tgatatagtt gttaactttg ctgctgaatc tcatgtagat
    15121 aggtcagttg agaatcctga tttatttatt aaaacaaaca ttataggaac acaagtactt
    15181 ttagatgcat ctataaaata tggagttaaa aggtatcatc aaatatctac agacgaagtg
    15241 tatggtgatt taccaattga tagaaaggat ttattcttta cagaacaatc ttctatcaat
    15301 ccaagttctc catattcagc ttctaaagca tcagcagatt tattggtaag ttcttattat
    15361 agaacatatg gattgttaac aactatatct aggtgttcca acaattatgg gccatatcat
    15421 tttcctgaaa aattgattcc tcttatgatt ttaaatgctt tggaaaacaa acaattgcca
    15481 gtatatggga atggggaaaa tgtaagagat tggttacatg tatatgacca ttgtactgct
    15541 atagacttaa taatacataa aggaaatata ggggagattt acaatatagg aggtcataat
    15601 gaaagaagta atctagaagt agtaaaaatg atattaaatc ttttaggtaa atctgaagag
    15661 ttaatatcct atgtaaatga tagacctggt catgatttaa gatatgctat tgatgcaact
    15721 aaaatagaga atgagttagg ttggaaggca aaatatgact ttgatttggg tataaaagaa
    15781 actgttaagt ggtatataga aaatgaatct tggtggaaag ctgttttatc agtataactt
    15841 taaaatttag aaaggggcgt tttatattga atgagataag ttcactaatt aatatattag
    15901 cattgactca attatctggt agtaatacta caaatcaatg taattgtaca tataataatt
    15961 catctggatt tgatatgatt atgatgacct tgttaaaagc attatcacaa aataatcaac
    16021 atacttctaa tagctacaat ttattgtcta attctgagaa tttatttaat gaacttgata
    16081 atgtagtaaa taataccact agtagattta tagatgttga tacaaaagat aaaaatgtaa
    16141 agttgagaat tgaaaatgct gtaaatcaag catcaaaaaa atataacgta gattcaaatc
    16201 ttataaaagc aataattaaa gttgaatcag attttaatcc aaataccgtt tcttctgctg
    16261 gtgctaaggg tcttatgcaa cttatgccag aaaactgcag agacttaggg gttacagacc
    16321 cttttaatat agaacagaat atagatgctg gaactagaca tattaaggaa tacattgata
    16381 tgtttggagg gagcatagaa atgggattga tggcatataa tggtggtcct ggaagaatga
    16441 aaagtagagg agtagaatca atctctgacc tgtataaaat gccaaaagaa acacaaaatt
    16501 atattcctaa ggttatgaaa tattatagag ggtaggcttt attatgggaa taaatataga
    16561 taagctaaac ataagtccaa gtataaatat ggaggttaaa aaactaaaag agagtaataa
    16621 attttctgac aaattaaact ctttaaaaca atcttcttca ttggaagata taaaacgaaa
    16681 tttggaaagt gtaaaaaagg caggagaaag gcttgtactt actcaaaact atggcgatat
    16741 tagtatatat aaaaatgcag taaaagaata cctaaaatct gttgtagata atatgtattc
    16801 acttgataaa aatagtagct tttgggagca tcagtattat aagaatgtaa agattgttga
    16861 tagtaaactt gaggatatga ctagtaagtt aattagtgaa gaaaaagaaa acatagatat
    16921 tgtttctact gttgacatga tacaaggctt attgatagat atgtatagat aaggttatac
    16981 aaggagaaaa attttatgaa tagcaaaaaa gaggatatat atgaattgaa ttttgggcaa
    17041 ttaatagaag aaaaatctgg tgttatagaa gaaaatcaaa atgtttttaa tgcaaaagta
    17101 aaggtagctg tttctatagg aaatacaaca gaaacaataa aaaatatatt agatttaaaa
    17161 gaaggctcta taataaaatt aagtaagaat gtggatgaaa aattagatat atatgcaaac
    17221 gatagattgt ttgcttatgg agaaagcatt attacaaatg agaagttatc tgtaagatta
    17281 tctaaaatag aagattcaga tgtaaattag cattttaaat atatcccttt agtctattct
    17341 gtatgagttt agctaagggg atatttttat aaaaaattta aaatatgtgt gaattatata
    17401 aatattttta ttttatgagc gataactaaa ttataagtat gtaattttgg aggtgaaact
    17461 atggatatta aaaatgtaag ttcaaatatt gtagtgaata attatttaaa gaatacatct
    17521 ggtataaaaa aagtggaaga ggttaaaaaa attaataaaa cagacaagat agagttatct
    17581 aaatttgcaa aggaaatggc aagtgtgtca aatatgagtg atgagaatat tagaaataaa
    17641 aaagtcgatg aaataaaaaa tgcattagaa aatggaactt acaaaataaa accaaaagaa
    17701 attgcgaaaa aaatgataga ggatatgcga ggataaagtc atgattacag acttaaaagt
    17761 tataatatat gaagaaaaaa atataattag taagatgatg gaattattaa aagaacaatt
    17821 tgattatata gttgaaaaag acttagaaaa tttaaatatg ttaaatccta aactagaaga
    17881 tattagcagg gagcttgcat ccatagaaat aaagagaaga caactttttg gtaatgatat
    17941 ttcaatgagt gaagttgttg aaaatagtaa tgatggatat ttaaaagaaa tatatacaga
    18001 cttgaaacgc attcttgctt tagctaaaaa tcaacaagaa tcaaatgatt ctctaataaa
    18061 gaaagaactt atttttacga aaaaaatgat taatttcata aaaccagctg ataagcaaac
    18121 tacaacttat aattcttatg gaaatattag aaaataaata ttaggaggag aaatatggca
    18181 ggattatttt ccattttaaa tacagctaat tcaggaatga atgcgcacca aaaagcaata
    18241 cagacaattt ctcataatat aagtaatcta gatacagacg gttattctag gcaaaggacc
    18301 gagtttgcta caaatagtcc aatgtatatg cctagtttga gtaattcaat tgcaagagga
    18361 caattaggga ctggagtaca tgtaacggat gtaacaagag ctagaaatag tttttatgat
    18421 taccaattta gggcagaagc tcataagtat ggtaagacag tttcaaaata tgattattac
    18481 aatactatag aaactatact aaacgaacca tcagactatg gaatatctgc aggtatagat
    18541 gattttttta aaggatggga gtcattatca aaggatataa atagtgacag taaaaaaagt
    18601 ctagtggtag aaaacgcatc taatttagca actttaataa gtgaaagtta taagaagctt
    18661 gataagttaa aagaagatat agattctaat atagacactg agataaaaca aataaatgac
    18721 atgttaaaaa gtcttgaaga tttaaataaa tctattgata ttatatctgg ttctggctct
    18781 acaccaaatg atttgcttga tgaaagagat agaattttag ataacttgag ttttaaacta
    18841 gactttgaaa atagtgatgt taaaaatatg cttgcagatg gtaaattaga acttaatgaa
    18901 ttaaaaaatc cagatggaac ttggaaaaca ggtatatcag ggaccttaca aggcttgttt
    18961 gaaatgcatg gtaagattga tacatataaa agcgatttaa aagatgtatc tgatggatta
    19021 gctaaacaga taaatgatgt ttataatacg tctgcaggaa taacagtaac agattttttt
    19081 ataacttcaa atgtagatgg tgaagatatt ataaagataa atccagctat aaagagtaac
    19141 ccaaatgaat taaagcttac tgctgatgaa gcttcaaaaa ttgctaagtt aaaggatgaa
    19201 aaaatagata ttggggtggc tggtggaaaa gtaagtacaa taagtgacca ctataaagcc
    19261 tttgcagaat caatcggttt agattctcaa aaagtaaatc aagatgaagc aaatcaacgt
    19321 aaaattataa ataatattga taactcaaga atgagtgttt caggtgtatc tttggatgaa
    19381 gaaatgacag agcttataaa aatacaacgt tcatatcaag ctagtgcaaa agttatgtca
    19441 acagctgtgc aactactgga tgttgtaata aatggaataa tttaatagaa gggggaattt
    19501 ataatgagag tcagtacagg tatgatgtct agcagctatc taaactcact acaagataat
    19561 ctacaaagat tggataaggt taatagacaa gttaatacaa caaaagaaat aaacaagcta
    19621 tcagataatc catataaagc aataaagata ttaaactcaa aaagtgaaat aaagactatg
    19681 gagacttatg ttgagaattg taaagataca gggaattggt tagaaactac tgatactgca
    19741 ttagatcagc ttggaaaatt attatcagac ataaagaaaa ctttggtggc aagtggtgat
    19801 ggtgcatact cagatgatga attaaagact cttagtaatg aaactaatga aaaaatgaaa
    19861 gaaattgcaa atgctttaaa tgcaacacat gaagggaagt acatattttc aggttcaaat
    19921 acgggggtac cacctgttga atgtatagat aatgctgatg gttcagtgag tttaaagttt
    19981 aattctagtc taaatcttaa taaattaaat gactctttga gtatggatat agctcaagga
    20041 ataagtgtag attataacgt taaattgagt agccttggat ttgacccaac aaaaactgac
    20101 ccatttgaag agttaaataa catatcaaaa aaattagcaa atccagatgc tgatgctata
    20161 aaagagctta caacaacttg tttgggcgat atggaacaac taatagaaaa tacagtaaat
    20221 gttaggtcta tatatgggac aaaagcaaat acagtagagg ctatgaagga aaaaaacgat
    20281 gatagtttga tacagttaaa ggatattttg tcacaaaatg aagaaataga ttatggtgaa
    20341 aaacttgttc aattaaaggc agcagagtta acttatcaag cttcactaca aacaggagga
    20401 aagttattta atgtatctat attagattac atataataaa ataacgcaga tatgatgaag
    20461 gttacattaa aaaaaggtat attaggtttt gaaaatttaa aagaatatga gcttttagat
    20521 atagaagata atgattattt aaaagaattt aattctactg aagaagaagg tataggattt
    20581 gttgttgtat ctccatttga gattatcaat gaatatgaaa tagttttaaa tcaggaaaca
    20641 atagaaaaac tagaagtgaa atctccaaag gatgttgtat tatttaatat aattactctt
    20701 ggtcagacat ttgaagaatc aactgtgaac atgaaagctc ctatagtgat aaatataaaa
    20761 actaattttg gaatgcaaat aattttacag gatgataaat attcaataag gcatccatta
    20821 ttgagaggtg ataatggatg ctagtaattt caagaaaaaa agatgaagca gtacttatag
    20881 gagaaagcat agaagtaaaa attgtaggag tagatggaaa caatgtaaaa ctagctatat
    20941 cagcgcctaa caatataagt atattaagaa aagaaatata cgaaaaagta aagagtgaga
    21001 atataaaagc tacaaataaa aatataaaaa ttttaaagtc attaaaataa taagcttgga
    21061 ggaagtaata tatgtatgga gaaaatcctt ataattctta taaacaaaat gctgttttta
    21121 tggcatcaaa ggaacaattg ttacttatgc ttgttgatgg agcagtaaaa tataccaaaa
    21181 tagccagagc agctatctta gataaaaata cacagaaagc gcatagagaa cttgtaagag
    21241 ttcaggatat atttacagaa ctaatggtta cattagacca aaatgcagga caatgggcaa
    21301 aggatatgta caaagtttat gattttgtaa gatatgaact ttcaagagcc aatattagaa
    21361 aagacatcca ggtaatagat aatgtgttac ctgtaataga acaaattaaa gatacatggc
    21421 atgaagctga taaaaaaagt aaagaagaaa gaagtaggta caaataatgg aattaaattt
    21481 agctgaatta gataagttat caaaagagga attactattg atgcttgtag atggcacagt
    21541 aaaatatacc aatatatcca aagaagctct tttaaataac gactatttga aagcacataa
    21601 tgaacttgta agagttcaaa atatatttac agaactaatg actacattag accaaaatgc
    21661 aggacaatgg gcaaaggata tgtataaagt ttatgaattt ataaaatctg aattagcaat
    21721 agctgatgaa aataaagata ttaaaataat tgatgatata ttgccaatag taaaacaaat
    21781 tagagataca tggcatgaag tttacaatca actaaaaata taattagatg gagggataaa
    21841 ggtatgtcaa gtataagtcc tataagagtt acaggtcttt caggaaattt tgatatggaa
    21901 ggcataatcg aagctagtat gattagagac aaggaaaaag ttgataaagc aaaacaagaa
    21961 caacaaatcg ttaaatggaa gcaagaaata tatagaaatg ttatacaaga atcaaaagat
    22021 ctttatgata aatatctaag cataaattct cctaatagta tagtaagtga aaaagcatac
    22081 tcttctacaa gaataaccag ttctgatgaa agtattatag tagcaaaagg ttcagctggt
    22141 gcagaaaaaa taaattatca atttgcagtt tctcaaatgg ctgaaccagc aaaatttact
    22201 attaaattaa attcaagtga acctattgtt cgacagttcc ctccaaatgc cagtggagcg
    22261 agttctttaa ctataggaga tgtaaatata ccaatatctg aacaagatac tacaagtact
    22321 attgtaagta agataaactc cctttgcgca gataatgata taaaggcttc ttatagtgag
    22381 atgacaggtg aattgattat ttcaagaaaa cagactggtt cgtcatcaga cattaattta
    22441 agagtaattg gaaatgacaa tttagctcag caaattgcta atgataatgg tatcacattt
    22501 ataaatgatg ctggtggaaa caaagtggca aatgtatatg gaaagaatct agaagctgat
    22561 gtaactgatg aacatggaag agtaactcat ataagtaaag aacaaaattc atttaatata
    22621 gataatattg actataatgt aaattcaaaa ggaactgcaa agttgacttc tgtcactgat
    22681 actgaagaag ctgttaaaaa tatgcaagca tttgtggatg attataataa actgatggac
    22741 aaggtctatg gtttagttac tactaaaaaa ccaaaagatt atccgcctct tacagatgcc
    22801 caaaaagaag atatgacaac tgaagaaata gaaaaatggg aaaagaaagc taaagaaggt
    22861 atacttagaa atgatgatga gttaagaggt tttgttgaag atattcaatc tgcatttttt
    22921 ggagatggaa aaaatattat tgcattaaga aaactaggta tcaatgaaag tgaaaattac
    22981 aataaaaaag gtcaaatatc atttaatgca gatacttttt caaaggctct tatagatgat
    23041 agtgataagg tatacaaaac actagcaggt tattcttcga attatgatga taagggaatg
    23101 tttgaaaagc taaaagatat tgtatatgaa tattctggaa gttcaacttc taaacttcct
    23161 aaaaaagcag gtatagaaaa aactgcttct gctagtgaaa atgtatattc aaaacaaatt
    23221 gcagagcaag aaagaaatat aagcaggtta gttgaaaaaa tgaatgataa agagaaaaga
    23281 ctttatgcta aatattcagc cttagaatct ttgttgaatc agtattcttc ccaaatgaat
    23341 tatttctcac aagcacaggg taattaatta tggataaatt aaaagaaaaa ttaaatttat
    23401 ataaagatat atctttacaa attataaatt tgattgaaaa ggaagaatat ataaagataa
    23461 gtattaagtt aggtgaaaga caagagatta taaatagtgt aagtgaaatt gatagaaatg
    23521 attttataca gctttataat agaatggaat taattgaaat tgatagcagg ataagagata
    23581 ttttacaagg acaattatta gaagtaaaaa aagaattaca tgagtataag cttactaaac
    23641 aagtgaacac aatgtattat aacttaaata gagaaaaagt aaatattttt aataaaaaag
    23701 tataaagtta tgtaccatct tgtccgatta tataaatgaa gaattaataa aaaagattaa
    23761 aagtagaaat gacaaggatg tcaactatac taaggagggt aaaataatga gagttaatac
    23821 aaatgtaagt gctttgatag caaataacca aatgggaaga aacgtaaatg cacaaagtaa
    23881 gtctatggag aagctatctt ctggtgttag aattaagaga gctgctgacg atgctgctgg
    23941 gcttgctata tctgagaaaa tgagagctca aataaaaggt ttagaccaag caggaagaaa
    24001 cgttcaagat ggtatatctg ttgtacaaac agcagaaggt gcattagaag aaactggtaa
    24061 catactacaa agaatgagaa ctttatcagt acaatcttca aatgaaacta atactgctga
    24121 agaaagacaa aagatagctg atgagttact acaattaaaa gatgaagttg aaagaatatc
    24181 aagctcaatt gagtttaatg gtaagaaatt acttgatgga agttctacag aaataagatt
    24241 acaagttgga gcaaactttg gaacaaatgt tgcaggaaca tctaacaaca acaatgaaat
    24301 aaaagttgct ctagtaaata cttcaagtat aatgagtaaa gcaggtataa caagttctac
    24361 aatagcaagc ttaaatgcag atggaacttc tggaacaaat gcagctaaac aaatggtatc
    24421 tagcttagat gtagctctta aagaattaaa tacatcaaga gcaaaattag gggcacaaca
    24481 aaatagacta gaatcaactc aaaacaactt aaataatact atagaaaatg ttacagcagc
    24541 tgaatcaaga ataagagata cagatgttgc ttcagaaatg gttaacttat ctaaaatgaa
    24601 tatattagtt caagcatcac agtcaatgct tgctcaagct aatcaacaac cacaaggagt
    24661 tttacaatta ttaggataaa ataattttgt atattggata tagaagaaag ggtaaggctt
    24721 tgctttactc ctttcttttt ttacaaatat acttttgaaa ggaaaattta tatgttactt
    24781 agtatagtta tgattgttaa aaatgaagaa aaaatacttg aaaaaacttt aaaatcatta
    24841 accacattaa gaaattctat agaaagtgaa cttataatag tcgatacagg ttctacagat
    24901 gatactataa aaatatctaa gaagtacacg gaaaaagtgt attttcataa ttggaataat
    24961 gatttttcta gtatgagaaa tatatcaata tcctatgcta aaggtgaatg gttacttata
    25021 ttagatgcag atgaaatact aattgattct tcaactattg taaaattttt taaagatggt
    25081 cttgataaaa aatttaattc tgcttcaata caattaaaaa atctttactc atatgataaa
    25141 aaattatatg gatattgttc agtgcttaga ctttttaaaa acgttgattt tagataccat
    25201 ggtaaagtac atgaacaacc aatatacaaa aaccctatat ttaataatgt tgctgatttt
    25261 gaacattatg gatatttgtt tgaagatgaa gaaataagat taaataaggt gaaaagaaat
    25321 gaaaaactat tatttcaaga gttaaaagag aatgataatt ctccatatac aaactatcaa
    25381 ttagggaaaa attttattat attaggaaaa tatcaggagg ctttatacta tcttgagaaa
    25441 tcttatgaat tatattctaa actagaattg gcacctggtt acttgataac aagccttgta
    25501 aaaacatatt tatatttagg aaaacataag aagtgtgaaa gattgtgctt aaagtatatt
    25561 aagaaagaca tgaataatat tgatatatat tattatattg cacaagcaca agtagattta
    25621 ggaaagtatg aaaatagtat agattcgtat aagagatata tttacttgtt agataattat
    25681 gaaataagta cacaagcaaa tagtttattt tcagacactg atactgtagg gcttagagat
    25741 gaggctatta ttacactgat aaagatttat tataagcttg aaaaatatga gtttgtaatt
    25801 tcagagtata ataacattga agatgtggaa aagaaaaaga atgtgtactt tagcttattt
    25861 atgtcactat ataaattgaa tagatttgaa gatataaaaa attattataa agaagtttca
    25921 aattctaaaa ttgaaaaaaa tagtttttat agaaatatag aagaagttat aaataatatt
    25981 aaagaggatg aaaaagagta tgtatataaa atcctttgtg acattgaagg taattatggc
    26041 attttaaatc aaataaggat aaataaatgt gtatctatag ataaatgtaa agaaatactc
    26101 aatgaagaaa aggaaataat ttatgcatat attataaaaa tagcttttga agagaagatt
    26161 gatttactag atatttttta tgaaatggaa tatgtatgga tagagaaata tctaaaatat
    26221 cttttagcat tagaaagaag ttttaattta gtactatata agtacataat tagtcgtcca
    26281 aatacatcag atatggaaaa gataagagta tataaagcta tatctaaagt agttttagaa
    26341 agtataagct taagtgatga aaaatataag gagatttttt atctttatat aatgtattct
    26401 tataagtaca taagatacat atatagcaat ttaagtgatt ttgaattaat aaaatttgtt
    26461 tgtaatgatt tagataagtt cactatagaa tttaagcatc tacttgatat taaaaataaa
    26521 gtaaataatg ctaatgaaaa attagagtat atacatagta tgaggaaatt actaaatgag
    26581 tatccttttt ataataaaat aataaaaata cttataggtg aattagaaga aaatataaaa
    26641 gaaagtgaag aatttaagga tttaaaagat agttttatta ttagtataga aaatatgatt
    26701 gaaactggca aaataaatga tgcgaaatta ttggtagatg actattcaaa aacatttgtg
    26761 gatgatataa agattttaaa tataaaggga attttataca tgtttgaaaa taagcttgat
    26821 aaagctgatt ttatgttaaa aaaggcatta tcattagata tagaaaatga agatactata
    26881 tataatatag aatacttaaa aagtttaaga taagtcaaaa ataggagaat tgaagaatga
    26941 atataccaat agtggttaat gaaaaatcta aagacataat aaaagattgg caatatacat
    27001 gtacaaatga agatataaaa tttacaagca taataatatt gacatataat caaatagaat
    27061 atactaaatt atgtattgaa agtattagga aatttacccc aaagaataaa tatgaaataa
    27121 taataattga taataactct tctgatggaa cagttgagtg gttaaaggga caatctgata
    27181 taaaatctgt gtataataac aaaaacttag gatttccaaa aggatgcaat caaggtattg
    27241 agttagctac tggtgaaaac atattgttgt taaataatga tgtaatagtt actccaaatt
    27301 ggctctataa tttgaataaa gctttatggt ctcaagataa tataggtgct gtaggaccta
    27361 ttacgaatgc atgttcatat tatcagcaaa ttgatgttga ttataagtca attgaggaca
    27421 tgtttatatt tgcacacaaa ataaataaat ctaatattaa cctatggaaa tatagattga
    27481 agttagttgg attttgcatg ttaataaaaa aaactgttct ggataatgta ggggttttag
    27541 atgaattatt cacacctgga aattttgaag atgatgattt atcttttagg atgatagagt
    27601 caggatataa gttattatta tgccaggata cttttataca ccattttgga agtgtgtctt
    27661 ttaaagaaaa tcgtaataaa tatacagata taatgtatat taatagaaga aaatttgaaa
    27721 ctaagtgggg atttaatcca gaatatagtg gaatgataag aacagatttg atttctaaaa
    27781 tagatgatag tttggaggca aatattaatg ttttagaaat aggatgcggt ataggggcta
    27841 ctcttttaga aataaaaaat atatataaaa atgcatcaat atatggaata gaaattagtg
    27901 agtcagcagg taaacttgca aagtatagtg cagatgtact tatagcaaat atagaaaaag
    27961 taaaattgga ttataaaaaa gatttttttg attatataat attgggagat gtacttgaac
    28021 atttgattga cccatggaag atagtcaatg aattgaaaca atatctaaag aaggatggtt
    28081 atctggtatc tagtatacca aatattatgc atgtttctgt tttaagaaat cttgtgttag
    28141 gtaattttac atatgaagat tcaggaattt tggacaagac tcatttaagg ttttttacat
    28201 taaatgagat aacaaaaatg tttaatgata gtaattacat aattaatagt attacagcta
    28261 ctagtatgcc actttcggaa attgataata gcttcattga tacaatatgt aaggaaagct
    28321 gtaaagaaat gagacaacag tacgaatctt atcaatatat agtaaaagcc tctaaaatcc
    28381 tagatactaa aagatattct agtattagta tgattaattt aaaatacgct ttaatgagaa
    28441 ttgataatga attagatgta gatagtaatt taagttatat atttgataat tataatataa
    28501 ctgaaccatg ttttgttgat gatattgaat atctaattga tacatttgtg ataaataaag
    28561 caactctatt aaatagatta ggagttgagg catttaacag aggtctagga gattttagta
    28621 taaacatgtt tatgacagta ttagagatgg atagagatga tattgactca atatataata
    28681 taacaagtgt attatgtgaa ttagaagaat ttgatactgc ttataattta attaaaaata
    28741 gtagcgaaaa agtacagagt gaaagtgata tacaagagat actttgtttt attgagggga
    28801 agaaatatga ataatgataa gatatgtttt ataacctgtg taaatgatga aaggctttat
    28861 gaagaatgtc ttgtatatat aaatagccta gacattccta aaaactttag tatagaaaca
    28921 gtagctttaa gaggagccga ttctattgcc tctgcttata ataaagctat gagagaaaca
    28981 gattctaagt ataaggtata cttacatcaa gatactttta taattaataa aaatttcata
    29041 catgatattt tagagatatt taaaaaaaat acagatgtcg gaatgattgg tgtagctgga
    29101 gtaaaagata tgcctgtgaa tggaatttgg tgggattctg ataaaaaagc agggaaagta
    29161 tatgatagtc atacaggtaa aatggatatt tatgaattta gtaactttga agaatgtttt
    29221 aatgaagtta tggctataga tggattaatt atgattactc aatatgatgt attttggaga
    29281 gaggataagt ttgatgggtg gcatttttat gatttgtcac agagtatgga gtttagacaa
    29341 aataaataca aagttattgt accaaaacaa aataagcctt ggtgtatgca tgattctgga
    29401 gtagtaaata taaataatgg atatgatgaa tataggataa aatttgtaaa agagtacata
    29461 aatcttgatt tatatagaga aaatttttat gaatttgata agaagaaagc aagtaccata
    29521 cttaagagta ataagcatgt aatggattgt atgaaagagg agcttacaga acaattaaga
    29581 ttaaagaatt acaaggatgt tttaggtatt gtttctaaaa ttgctcattt ttctgttttt
    29641 aatcatactg gtttgtattc ttgtgaagaa tgtgagagag cattgctaga atgtgcaaat
    29701 aatttaccag aaatagaaaa tacgaaatta tttacaaaaa aagatttaaa aagaagaaaa
    29761 gtattacatg ttttaagtga aggatattca acaggaggtc atacaagact agttaaaaat
    29821 tggattgaaa ttgacaaaga ctctgttcat tcagttgtaa ctacatggca atcagataca
    29881 ctgccagact ggttattaaa agcaataaat gatagtggtg gcgaagttat atctttggat
    29941 ttaatttcag atgattattt aaaaagagct gtagctttga ggaaaatagc ttatgactgg
    30001 gctgatatag ttattttata tgttcatatg tgggatacaa tacctgttgt tgcttttgga
    30061 gttgatggga aaatacctat tttacatgtt aatcactatg atagtacttt ttggttaggg
    30121 acaagtattg cagatattgt cattgatttt agcgaagcta gtcaattaat tacattagat
    30181 aaaagaggca tagataaatc tgaaatatta ccaatgccat taaaagaaaa agaagtattt
    30241 tcaaaatctg aagttagaaa acgatataat ttagaggatg atgtgacaat aatattgact
    30301 attgctagtg aaagaaaata caatagtgta aatgatatta attactttaa aattgctaaa
    30361 acattgattg aaaatacaga taatacaatt attattgttg taggagttga tacagagaat
    30421 ctctattgga aaactttaag taacgttaca gataatagag ttatatgttt tggaacaaca
    30481 gcaaatatag acgattttta caagatttcg gatatatata tggagtcttt catggtagga
    30541 agtcatactt caaaactaga tgcaatttta tatgatttaa ttcctgttaa gtttaaaaat
    30601 gaagtttcac caatttttac aaatatgtgg aattttatag ataagtttga ttataagaat
    30661 ataaaagagg ttatagatat gataaaaagg tacaatacta gtgaatttga cagggatagg
    30721 ataaaagaaa aacaatcgga aataaaacat tttgttatag ataaacactg tattgaaata
    30781 aaaaactata tagacaaaat atataatgaa gctaatcatc ataaagttaa gtatgattta
    30841 tataaaaatg atgaaatcga ggactatcag ctttttgtag cattatatgg ctatatggta
    30901 aaaaataaaa acatgtcata ggtggtaaaa tgaacaatat tcatatcaat aaatttgaaa
    30961 aaagctgtga aagaaactgg gattattttg tagaaaataa atctataaat ggtacatttc
    31021 tgcaaactag aaatttcctt aattatcatg aggagggaaa attcactgac cactcattaa
    31081 taatatgtaa aggtgaagaa atactagcag tcgtacctgc atgtgaaact tatgaagatg
    31141 gtgaaagaat atttttttct catttaggga gtacttttgg tggtattata ataagaagtg
    31201 gttttaatga tattaggcat gtaaatgcca ttacaaataa gttagaaata tatttacggg
    31261 aacaaaattt cgataaagta tatttaaaaa atacatcaag aatattttgt aaagaggata
    31321 cttcattact agaatatttt ttatttaaga ataattacat gttttatgat gaattaagta
    31381 tgtttttaga attaagtaag tataaagaag atatattagc caattttaaa tacagtaaaa
    31441 gaagagattt taaatattct ttgaagaata atcttacctt taaaagactg tatagtgatg
    31501 aagatatagt ttgttttttt aatttacttt ctaattgttt agatagtaaa ggagctaaag
    31561 taatacattc agtagaagaa cttttggagt ttaaaaataa tagattaaaa gatattgtag
    31621 aattttatgc agtatatatt gatgacatta tgatttgtgg gagcatggta tttaactttc
    31681 aagaaagagt ttatcatact caatatctag catcaaattc gacatataat aaatattatc
    31741 caatgatatt tttatactat aatctaataa aagatgcttt agagagaaat tttaagtatc
    31801 tttcttttgg aattagcact ctaaatagag gcaaatactt agacgagggt ttagctacat
    31861 ttaaagaagg ttttggttca agtgcaattt gtaacagaac atactataaa aatataaata
    31921 agtgagaatc atgttttata gagtttttag aatatagtaa atatctagta ctctaacatt
    31981 acataaaaat aattagttta taaagaaggt ttgtatatga aaagattaat gattttaggc
    32041 tcaattaagt attttgagaa tattgttata tcagctaaaa aagagggaat atatacgatt
    32101 gtttgtgata atagaattga tactcctgcg aaaaagattt gtgatgaagc aatagatgtt
    32161 gatgtatttg attttgataa aataaaaagt atagctatag ataaaaagat agatggaatt
    32221 ttaactggat ttacagattc tttaatgaaa ccatatgttt atattgcaaa tgaactaaat
    32281 ttaccatgtg tgatttcttg caatcaactt gaatctgtaa ctaacaaggc tattatgaaa
    32341 aaatatttta aaaataatgc aatacctaca actgattact gtattattga aagtctaaaa
    32401 gatttagaaa aaatacaagg attaaatttt ccacttgtaa taaaacctgt tgatggttat
    32461 ggttcaagag gtgtatattt tgtatcaaat atagaagaat taatagaaaa atttgaatat
    32521 tctaaggaag cctcacaaga tggacaagta ataattgagg aattttatga aagtgaggaa
    32581 atacaaggac tagcttgggt tcataatgga gagtcacatg tattttatat tggagataga
    32641 gagcttgtaa acatacatac aggaagagct ggaaaaccag atagattact ttatccatca
    32701 aagtattgtt ttcaatatga ggaggaaata aaatctatat atcaaaatat aacacaggct
    32761 tttgatataa aaaatggacc tctttatgta caaatgttag tgggagcata tggaataaag
    32821 gtatctgaag taatgccaag attacctgga ggttgtgact atatggctat ttctcaaata
    32881 acagatttag acataggaag tttatttgca aactttagtg tgaataatga agtaaatttt
    32941 gatgaaataa agaaatataa tatgcatcta tcaaaatgtg tatatgcatt accaatatac
    33001 ataagacccg ggacaataaa aaagataaat aatatagata aaatagaaaa cttagattat
    33061 gtcactcaat ttctattact agtaaaagaa ggagatacaa tagaattata tgaagatgag
    33121 agacaggatt gtggaagagt ttatggaata gcaaataata tatttgatgc aaatataaaa
    33181 aagaaatgta ttcatgaaat gatagaaata cttgatgaaa ataatgaaaa tatgatagaa
    33241 aatttttaaa taagggagta tgatagatat gaagctaata actcatgaac aaattatgtc
    33301 attaaacatt tctcagaaac aaggatatga gtggataaaa gaatctttct taacaaaaaa
    33361 agattgtcaa ttaccaccaa aaataagcat ggtagaagaa ggacatgtat tttacaatgt
    33421 aatgccatcc ctaaatcata atcatatgat tggtggaata aaaatggtta caagattgcc
    33481 agggagaaat ccaagtttaa actcagagat atttctatac aatatggaag atgctaaatt
    33541 aaaggctatt ttggatggta attatattac agcttttagg actgctgctg ttgctgcctt
    33601 ggctatcgat acatttgcta aaaaagattt taatactatt gctattatgg gtttaggtgt
    33661 aactgcttct gctataatga atatgctttc atatactctt aaagataaaa atataaatat
    33721 aaagttactt aaatataaag accaagcaga gttgtttgta aaaaagtatg aaaaaaatga
    33781 acaatttagt tttgaaatat gtgatacaca tgaagaactt attgtagatt cagatgtagt
    33841 tatttctact gtgacatata cagatgaata tttatcagaa tttaaatggt ttaaaaaagg
    33901 tgctttatta ttacctgtac atgttatggg atttggaaat gttgattatc tatttgataa
    33961 agtatattta gatgattata atcatttgaa acatctagaa aatatagata gatataaata
    34021 ttcttgtgaa ttttatgatg ttttaagtaa acaagctaaa ggaagagaaa atgatgatga
    34081 gagaataata tcttacaata taggtttgtc aatacatgat atattatttg caaataaaat
    34141 ttacgaattg tgtgaaacac taagctgtga agaaatagaa ttaaaagaac caaaagaaaa
    34201 atattggata tagtttattt cattcatatt tacaatagtt tattgtaaat atgaatatga
    34261 agtaatatat tgttagaaaa ttagcatgaa taggatagat aaatttatgg ataaatataa
    34321 tttaattagg ttcgtatcta ttggaaacga atatatagga aatttagttg cattagaatc
    34381 aaataaagta ataccttttg atataaagag agtttattac atatatgatg taccaaacaa
    34441 tgtaaagaga ggatttcatg cacacaaaaa cttggaacaa gtactagttt gcctaaatgg
    34501 aagtgtgaag ataaaatgtt atgatggtgt aaaagagact atatttaatt tacataaaaa
    34561 tgatgttgga ttatatatag gaacgggagt gtggcatgag atgtttgatt ttgatgaaaa
    34621 aactgtttta atttctatag catctgaaca ttataatgaa gaagattata ttagagacta
    34681 taaagaattt ttaaagtatt taaataatgt gtagttatta tacaattagt gatgtatttt
    34741 cttaattaaa taaatttaag atagccacat agagaataga tagattagga tgtgaattta
    34801 ttgttggtta aatttaataa tttattaaaa cagttccaaa ctcataaaga agaatatgaa
    34861 cagaagatta aggatgtaat ggaaagtgga tattatatcc ttggaaatga agtaaagcat
    34921 tttgaagaag aatttgctaa ctatgtaggt agcaaatatt gtgttggttt agccagtggt
    34981 ttagatgcac tgataattgc ttttagagca ttaggaataa gcaaaggtga tgaagtaatc
    35041 gtgcaatcaa atacatatat tgctagtgtt atgggaatca gtataaatgg agcaacacca
    35101 gtatttgtag aaccaaatga atattataat atagattcat ctaagataga agaaaagata
    35161 aatagcaaaa caaaagctat attagtagtg catttatatg gtcaagcaag taatatgaaa
    35221 gatatacaag aattatgtga taagtataac ttgtatttaa ttgaagattg tgctcaatct
    35281 catggagcta aatatgatgg aaaaatgaca ggaacatttg gtgatatagg ttgttttagt
    35341 ttttatccaa gtaaaaattt gggtgcattt ggagatgcag gagctatagt aactagtaat
    35401 gaggaaatag ctgataaagt gaaaattctt agaaactatg gctcagaaaa gcgatattat
    35461 aataaatttg tcggatataa ttcaagattg gacgaaattc aggcaggttt attaagagta
    35521 aaacttaaat atctaaatga aattgaaatt gaaaaatcaa aaatcgtaga tagatatctt
    35581 aatgaaatta ataatcccaa agtcatactt ccaaagatag aaaataattg tactcatgta
    35641 tggcatttat ttgttttaca agttgaaaaa cgagatacat ttatggaata cttaaagaaa
    35701 agtggtatag agtctataat acattatccg ataccaccac atctatctga agcatatgct
    35761 tatttgggat ataaaaaggg gagttttaat atagctgaga actattcaga taaagttgtt
    35821 agtatacctt tatttattgg tatggaagat gaagaagtta gttatataat taaaattata
    35881 aatgaatatt aaactactca taataatatc aaaaataaat tttttatata tcatttattt
    35941 aaatttcgtt aattgacatt tttaaatatt gatatattgt acaaataaaa ttgaaatata
    36001 tggaaaaata tagaattaag atgagatttg tcttatattt ttataaatta aaaatatttt
    36061 caaagagttt attataaaat tgtaaactat agtgtttata atctttgtaa ttattggtaa
    36121 ttaaatttaa aaaataaaat atttaattat acacaaaaaa gatatttaaa aaatttgtta
    36181 tttattgtat acttaagtta aaactaaata ggcaaatcta gagaaatcta gtgacgcaaa
    36241 gctataggga ctaaggttta tatacataaa ctatgtcagc cagttgccaa aaagagtcct
    36301 aggtgtattg tatacctaag aaaagtctat aatgactgcc tttttggcag tcattttgtt
    36361 atggaaaaat cactttattt cagtagaaaa tacacataat aacatatttt accaattata
    36421 acataaaata aagaaaaatt atctcaaaaa gttactattt tacaaaaaat gacttcttta
    36481 tttatttaaa ttaatcgcat ttccattaca ttaatacaat aatgtatcaa tttagtaaga
    36541 aaactaatat ttttttaatg aagtcccatg aaaatataaa aaaattttaa tatgtattta
    36601 aatatttctt cgaaaagttg ctcttttttg taatatagca acttcatttt ttttattaat
    36661 aaacaaaata gataaaatat attttgagga ggggtgagtg tgaatacata tgacattata
    36721 aaaatgggat tagatgcaac taatctaaga agtcagacaa tagcaaataa tatagcaaat
    36781 ataaatactg aaggatataa aagacattat ataacttttg aagaaacact aaatgatgct
    36841 atgcctaaaa tagaagaaaa aacagacaat tctaaaggtt taagagtaga tggtaataat
    36901 gtagacatag aagaagaaaa agtaaatcaa gcaatggcaa cattacaata taatgctttg
    36961 ataagttttg caaatggaaa aatagcaatg accaaatcaa taataagtgg gaggtaatta
    37021 gaagatgagt gtgttcagtg gaatgagaat atctgctagt ggattaacag cagaaagttt
    37081 gaaaatggat atcatatcat ccaatgtagc taatataaat gctacaagag gtccaaatgg
    37141 agaagcatat aggagaaaag ttgtttcatt tgaagaaaac tatgataata aattaggtat
    37201 gctaggagta aaagttacat ctgttgaaga tgataaatca cctcttaaag aagtatatga
    37261 cccatctcat ccagatgcaa agccaaatgg atatgtagaa tatccaaatg taaatatatt
    37321 ggtagaaatg acagatttaa taacagcttc acgagcatat gattctaacg tagatacatt
    37381 gaatgctcag aagtctatga tatcaaaagc tttggaaata ggaaagtagg gataaattat
    37441 gccaattaca caaattaaca atttaatgca aggtgcaaag ttagataatt tgattggaga
    37501 agcaaagcca ttaactctta ataataaaga agaaaaaaag aattttgaag atgtaataat
    37561 agatactata gataaagtta attttcaaca agtaacagca gatgatatga aagaaaaatt
    37621 tataaaaggc gaagatgttt ctatgcatga agttatgtta ataggtcaag aagcacaact
    37681 atctatgcaa tatttaatag aagttagaaa taagattcat gatgcatatc aagaaatgaa
    37741 taaggtgcaa atttagcctt ttgaacagga gtaataatca atgataacca gttttaaaaa
    37801 taagattaag gagcaagttg ctaaatctag gacaaagata agttctttaa aaaagggaac
    37861 taaaatagca ttaggtatag gggttattgc tatatgttta gcgctagtat ttacagcaat
    37921 atctgcgagc aaaaataagt atgcagtctt attttcagaa ctagatgctc aagatgctgc
    37981 agatatatca gcagagctag agtctaaaaa gatagatact aaggtaaaag gaagtacgat
    38041 ttatgtacct agaacagaag ttgataagtt aaggctggag ttagcgccaa gtcttacagg
    38101 tggttctaaa ggatttgcac taatggatga tagttcatca atagggctta ctgatgaaga
    38161 atttcagatt aagaaacaaa gaatgataca aggtgaaata gaaaaaacaa taaagagttt
    38221 tccacaagtg gaagacacta gagtacatat aacatttgga gagtcatcag tatttgagaa
    38281 ggaggctgtt ccagggagtg cagcagttta tttgatgcta aaaccaggga cgactttaga
    38341 aaaaaaccaa ataaagtctg ttatggcact tatatcagga agcgttacaa atataccaga
    38401 gaaaaatgtt caggttattg ataacaaaat gaatttatta tctgaaggta ttttggagga
    38461 tgaaaatgtt gatgaaaata atcaaccaat aaatagtggc attagtgttg ataaaaataa
    38521 ggcatctgaa caagcattga ataaacaact agaaaagtct attttagaaa tgctagaacc
    38581 gatatttggt aagggaaagg tgaaagctac agtaaatgct gatttaagtt ttgatacagc
    38641 tgaaaaaact gagataaaaa ttgaccctga taaagttgca ataaaggaga caaggtcgaa
    38701 aaattcatct aaatctgctg caaataatgt tcaaggtgta gatgctaaca tgaataacca
    38761 aggaaataac aatggtacta atacagaaga tagtttagat gagagttttg agtatgaaac
    38821 aggtaagatt gaaactcaca ctatagttgc gcctggagaa cttaatagat tgacagcttc
    38881 tgttgcaata gatggaaaaa tagctaaagg tgttcaagct gatgtagagg atattgttaa
    38941 taatgctata ggtatggatg gagcgagggg agatactctt agcgttgttt caatggtatt
    39001 tgacccagaa ggtaagaaag aggctaaaca agagctggaa gagattaaaa aagaaggatt
    39061 taaaaagaac ataataaaag cagttattgg agtagtagca gtaatagcag ttggagctat
    39121 tggatactta atatataaga aaagaagtga acgagaagat atggattatg aagatgaaga
    39181 ttatatttct aaatctgatt ctgttgatag gaaggataat cctctattag gagatgaccc
    39241 agaactgaca ttagaggaag ctgttaaaat gtatgcagaa gaaaagccag accaagtaac
    39301 agagataata aagacttggt taaatgagta agggggatag aaagtgtcca cggaaattac
    39361 aaatgttaat gcgtttgact taagtgatat aaatgaaagt gataagaatg agaaggtaga
    39421 aggtgagggt ttaaaaaaag ctgctgtatt attaatgaca ttaggcccaa atgtgtctag
    39481 tgatataata aaaagcttgc ctgataaaca agttcaaaaa ataggtttgg aaattgcaaa
    39541 tatttcttca atagcttcaa aagaaagaag agaaatctta aaagagtttg tagaaataaa
    39601 tagggcaaaa gattatgtta tagaaggtgg gcttgaatat gcaagagagt tattaagtaa
    39661 tgctttagga aatgttaaag caaacaaatt acttgaaggt atatcaataa atacaagtac
    39721 aaagcctttt aaacatatta taaaactaga tgtaaagaag atattagaaa tacttaggaa
    39781 tgaaagtgca caaactatag ccgttttact tgcacatatg gaaccagaaa atggagcaga
    39841 agttttatca tacttagatt ttgatttaca aaatgaagta gctatgagaa taagctcaat
    39901 gggttcaata tctccagaag taataaaggt aattgaaagt tcattagaag ataaattaca
    39961 ttcaataagt ggtgatgatt ttaatgatta tggaggggtg tctaccttag ttcaaatatt
    40021 gattaattct gatagaaaaa cagaaaagaa tattttggga actattgaag aacaaaaccc
    40081 ggatttagca gctcaaatta aatctcatat atttgtcttt gaagatattg tcaagttaga
    40141 tgatataagc atacaaaaag tgttaaaaga agtgactatg aaagatttag tatatgcctt
    40201 aaaaggtgta gatgatgaga tttcagatgc tatatataga aatcaatctt ctagagcagc
    40261 agatgcactt aaagaagaaa tggaaatggt tggtgttata aaggttacac aagttgaggc
    40321 ttctcaacaa aagatagcta atatcgtaag aaaacttgaa agagaaggtg ccataactat
    40381 tgtaaggaat tttggtggtg actttgttga ataataagat tattaaaagt aaaaaagcat
    40441 tatataaagg agtgctagat ttaaccataa tagataaagt tagagtggga gaatcaaatg
    40501 taaataaaga aaatgattgt agatttaggg atgaggtaat aaaagaagct atagaatctg
    40561 ctgagataag aaagaatgag atattagaag aagcaaaaat gaatgcacaa aatatagaaa
    40621 agcaagcata tgaaaaaggg tatagtcaag gtcaaaaaaa tggatatgaa gatggatata
    40681 aagagtcata tgatgaatat acccaaaaag ctaaagatga agcagatttt ataaaaaatc
    40741 aggcagactt aatactatta caagctaatg agaagatgac tgagtacata aaagaaaaaa
    40801 ataaagagat tgtaagactt gctattaata tggctagttc tgttctaaaa aagcatttcg
    40861 aagatgaaga atctatgagt gatttgttaa agcaaattat tcaagattat gaaggaagta
    40921 gttcatttat agtaagatgt aattctttct ataaagataa tatagagaaa gagtttgaat
    40981 tattcaaaga ggaaacatca ctaaaatcaa gtgtatttgt tattgcagat gatagtatag
    41041 ataaaggcaa tgctattatc caaaaggaaa atggaagaat aatagttggt attgattgtg
    41101 ctatggaaaa actaaaagaa gaattgcttg gtgaataaag tatgtataaa actgatatat
    41161 ctatagattt tgacaaaata aataagaaaa tagaaaattt agaactcact atatcagaag
    41221 gtagagtaaa gaagataata ggacttactg tagaagttga aggaataaaa gcttttgttg
    41281 gggagttatg cgttatatac aaccaagtta ataaaccagt gaattgtgaa gtagtaggtt
    41341 ttaaagataa agaagtcata ttaatggcgt tgggagaatt aacattgata gcacctggtt
    41401 gtaaagttat ttctaagggg atacctttaa gtgttatgtg tagtgataat ttactaggta
    41461 aagtacttga tggtcttgga aatcctatag acaactcaga tgctgtttta ggagatagat
    41521 acaacctgaa caatgaacct cctgacccaa tgaagagaaa aaagatacga aatataatgg
    41581 aaacaggtgt aagagcaata gatgcattta ctacttgtgg tgaaggacag agaatcggca
    41641 tatttgcagg aagtggagta ggtaagagta cgactctagg tatgattgct aggaatgcaa
    41701 aagcagatgt aaatgtaatt gctcttattg gagaaagagg aagagaagta ctagatttta
    41761 ttgataaaga tttaggtgaa gagggaatga aaaaatctgt tgttgtgtgt gcaacatcag
    41821 ataaagcccc tctagttagg ttaaaaggtg ctcttactgc tactgcaata gcagaatatt
    41881 ttagagacca aggcaaaaaa gtaatactta tgatggattc agtaactaga tttgctatgg
    41941 cacaaagaga agttggtgta gcaataggag agccaccagc tcaaaaagga tatacaccat
    42001 ctgtatttgc aatgttgcca aaacttatgg agagaacagg gacatcagat aaaggttcta
    42061 taactgcatt ttatacagta ttagttgatg gagatgactt taatgagcca atagcagata
    42121 caacaagagg gatacttgat ggtcatatag ttttatctag agacttggca aataagaatc
    42181 actatccttc tatagatgta ttaaacagtt taagtaggct tatgaatgaa attgcttcaa
    42241 aagaagacat aaaaatagcg tcttttgcaa gagacatgct tgctgagtat agagaagcag
    42301 aagatttgat aaatataggt gcatatgctt caggtacaaa caaaaaaata gatgaagcaa
    42361 tctattatca tgagcatatt attaattttt taaaacaagg aataaatgaa aagagttctt
    42421 ttaatgaaac tataagtagt ttaagaagaa tctttgaata aacaatttaa aaaaatattt
    42481 gaataggttg tgatggaatt ggataaaaat tttaagttta gattacagaa agttttagac
    42541 ttaaaaataa aagatgaaga agagataaaa atggaatttg ctaagattca acaaaaaaaa
    42601 atagatatag aaactaattt agaaaactta gaaagtaatt acagcaaata ttctataagt
    42661 aaaaataatg ataatgttca aaatcaaaaa ataactatta actatttgct agctttaaac
    42721 aactctataa tggatttatc agaagaactg gataagtcga caaatgaact tgaaaaagct
    42781 agaaaacagc ttataagcaa acagatagag agaaagtcat tagaaaaatt aaaagaaaaa
    42841 aaatatggac agtattataa agaagaacag ttaaaagaac aaagcactaa tgatgagttt
    42901 gcaagcatga gttatttgag aaatagacaa gttctataaa ataataggaa gtgaggatat
    42961 tattgtatga agatacaagc agattcttat aatattatta aagatattgg gaatagaaaa
    43021 aactcatcta agaataaaaa tataaaaaat aataaatttg aagataatct atataagaca
    43081 acagaccata aaaatgttaa agatagaaga gaaacaagta agcaaaatgt tagttcagat
    43141 agaccgggaa atagtgataa tgagtgtcat ttagatgaag ataaaagtaa tcacttagaa
    43201 gatgaaaata catatgaaaa agattgtagt aagaataatg aagctttaca gcaattactg
    43261 aatcttttaa aagagaatta taaaaaagat aaagatataa acactatatt gaataatata
    43321 aaaaaactaa gtatatcaga tgaattaaaa caagaactta agcttaataa aaatatattg
    43381 aataaaattg caaatcaaaa tttagagtta aatggagcta atacaaataa ccctaatgat
    43441 atagaaaatt tagacataat gaagttaaga gaacttttag aaaataatgc tagtgaagat
    43501 attcaagtaa ttagtaaaac tacagaaaat gatactaaaa gcatagactt atacagtttt
    43561 gatagtaata gaatggatac tcttaactca aataaacaga aagatgatag ttctgatatt
    43621 ttggaaaaac tagcagggtt aaatggaaat aagagtggaa attttggaca agtaataaat
    43681 aaaagcgttg atatgaataa aattaaaaat gataatgtta atttaaaaca gatagaatct
    43741 aatgtaatgg atgattctat aaaagctatt aagcatatga aaacaaatga tatacaagaa
    43801 ttgacaatta aattaagacc aaaagaatta ggagatatga acatacaatt attaaaagat
    43861 ggtgaaagta tgagagctgt tgtgactgtt ttcaataaag atgtctttga ctctataaat
    43921 aaaaacatag cagatttaaa gcaacatctt gaacttacaa atgtaaatat aaaagatgta
    43981 tcagtgcaaa tgcatagcga taatacaaat acatctgata cctttgataa agcatttgaa
    44041 caacagaata gacaaaataa ccaagaaaac atgagtaatc aaaatacaaa tgataagcgt
    44101 aacaatgtag tcatagaaga tgagattaaa gaaaatatta ttgatgatga tagagtagat
    44161 ttactagctt agtaaggggg aagtattgca tgtacggaat agcaacggct ttggcaggaa
    44221 ctcaggtaaa ctataggtca aataacttaa gccaacttga aagaaatatt aatgatattg
    44281 ccaacaaaca agttagaagt aaaggaaatc agacagtaac agacaatggg actccaatta
    44341 taggacaagg tgatgatgct gataaagact tgtttatgaa gattcttgta gcacagatgg
    44401 gtaatcaaga cccaatgaat ccacaagacc caacggagta tgtatcacaa ttagcacagt
    44461 ttgcaacttt agaaaaaatg tcagctatga atgataagct agatatactt ttagccttaa
    44521 gtaactctac tctaataaac tctgctttga gtatggcaac atcattaatt ggtaaagaag
    44581 tagaagtatc tggggaagaa gatgaagatg aaaacattgt aggagtagta aagtccacat
    44641 acataaaaga tggggaagta cacttagaat tagaagtaga tggaagtgac aaaactgtag
    44701 atgttaaata tgaaaaatta cttaaaatag gagaggtaga ggcaaattca ggagaagaaa
    44761 atgactcttc agaacaagaa tctccagaag aagtataata ttaaatggga aaagaggtaa
    44821 atttatgata aaagcaatgt attcaggtgt aagtggaatg aaagcaaatc aaacaaaact
    44881 agatgtaatc ggtaacaatg tagccaacgc tggaactact tcttttaaga aaagttcagc
    44941 tagaataaca gattcatttt atcaaactgt actatatgca agtgctccaa cagcggcatt
    45001 agggggaaca aacttaggac aagtaggtgt aggtagtaag atatcaagta taaacaaaga
    45061 tatgacacaa ggaaatgtac aaccaactaa tagaccatct gatttaatga tagatggaga
    45121 tggatatctt ccagtagtaa gacaaggaac agttatgtat acgagagatg gttcttttaa
    45181 tttagataca ggatttaaag cagctgatgg tgctttgaag aatattaaag gtggaagact
    45241 tgtaaatggt gatggatata tgttacaagg tatgatttat gatggggaat ataaagctga
    45301 tactggagaa tttgttaaaa agggtgatgc taaaggaaaa gagcctattg taataccact
    45361 tcaaagtata agtcctaagg atggaacagt tcaaaatgta ctagagtata atatatcaaa
    45421 ggatggaaca gtagaatttt tattgagtga tggtcaaaga actcaatttg tctatgatat
    45481 acctgatggt gctaaggtag gagatacacc tgctggaata gataaattaa aaggtacaat
    45541 acaaaaagtg caaacatatg cattccaaaa tccagcagga ctagatgcag aaggaggaaa
    45601 cttgttcaag ccatcagcaa actcaggaga cccacaaata gcaggagcta caggcacaat
    45661 aatacaagga gctatagaag cttccaatgt agatatagca gaagaattta cagaaatgat
    45721 aatagcaagt aggtctttcc aagcaaacag taaaataata tcgacatcag acgaaatact
    45781 tcaagaaata ataaatctta agagatagta ggtacatatt atgataagag taacggattt
    45841 aaacggaaga aaatacatat taaattcaga tcttatagtg agaattgact ccgtgccaga
    45901 aagcaggatt ctccttacaa caggagaaaa aaatcttgta aaagagtcag tagatgagat
    45961 agtagataaa gtaataaagt ataaaagaaa aataaatgta ggttatatta ggagtgaaaa
    46021 ataatgaaaa aaacagattc actaacattc attggactaa tattatctac agtattagtt
    46081 ttatttggag cagtaaaagg aagtagttca ggattgaaaa gttttttcga ttttgcatct
    46141 atattaatca cagtatttgg gtcatttgga gctttgatga ttacatttac tattgacgat
    46201 ataaaactta taaaagatgc tctacaatac tcattcaaga caatgaatat atcgaaatta
    46261 gacttattaa atcaatttaa aacactttct aaaaaagcaa gaagagaagg acttttatct
    46321 atagaaagtg atgtaatgaa aatacaagac ccatttatga aaaaaggatt agagttatgt
    46381 attgatggtc ttgaaataaa tgaaataagg tctatactag agctcgaaat agattctgtt
    46441 gaagaaacca ataacagata ttctaaacta tttaaaactt gggggacttt cgcacctgca
    46501 tttggtatgt tgggaactct aataggttta atacaaatgt tatccgacct tacatctcca
    46561 gatttaatag catctggtat gggaaaagca ttaattacga cattttatgg gtctttactt
    46621 gccaatatcg ctttgaaccc aattgcatat aatatagatg agaagactga aaaagaaatt
    46681 tatgtaaagg aaatgatgct ggaggggata ataagcatac aatctggaga gagcagcata
    46741 gtagttgagg aacgtttagc tacatacctt tcaaataatg agaagttaga aattatgaaa
    46801 tccaataaaa atacagaaag agcaatgagt aatggcgcgt aaaaaaaata agaaaaagga
    46861 tgagataaat ccagatgcat ggttggctac ttatgcagat acgataacac tgattcttac
    46921 attttttgtg ttattgtatg ctacatcagt tgtagatatg gaaaaattta aaaatatagc
    46981 agatgcgtta aaaggtcagt tttctgggac aagtatgttt agtacaggtt caagtacaag
    47041 tgagaaatct ccaatagaga gtgttttagt ggataaaaat gataagtcaa atttagaaca
    47101 ggatttaaaa gataaagtta ataattctaa cttacaaaat agtgtaacag tcaaagatga
    47161 tagtagaggg attttactag agctagatga ttcaatttta tttgactctg ggatttctga
    47221 attaaaagaa gatagtaaag ccactcttaa taaagtatac gatatgataa agatgatgaa
    47281 aaataagata gttatagaag gccatactga taatgtacct gtaaaatcaa gtacgcatga
    47341 atccaattgg gatttatctt ctagtagagc agtaagtgtt gtaagatact ttgtagaaga
    47401 aaagggcatg gaccctaaac tattttcggc aacaggatgt ggagagtacc aacctttggt
    47461 atcaaatgaa acagcaaagg gaagagctga gaatagaaga gttaatatac ttataactat
    47521 tgactaggag gaagaggttt gttcaaaaat aaaaaaatta ttataattat tttgatactg
    47581 attattttct cagcaggagc aggttttttt atattaggag aaggtggaaa taaacaaatt
    47641 gacaagattg caggtttgtt tattccaaca gggaaaggca aagatgaagt aaagaccgaa
    47701 aatcttgact tagaagagat ggtattgaag ctagctgatg aaggtgtaag atatttaaaa
    47761 gtatcaataa ctgtatctta tgattcttca tataaagata tagaaaaaaa tacgccttta
    47821 attagggata atattataaa ttgttttatg gcaaaaaggg cagatgattt tactgctgat
    47881 aatttaaaga acataaaaga agaaattaaa aatacattaa atatacaact aggtgaaaat
    47941 gtaattcaaa atatatattt cacaaatata gtagtgcaat aaaaggatgg taagacatga
    48001 tactttattt actaaaaata ttaatatgta tcccaataat tttaatattg atagtcttat
    48061 ctttaaaaat tagccaagga actttattaa aaaaatataa tgataaatat ataaaaatac
    48121 tagatattgt aaatataagt aaaaacaata gcataataat tttaaaaata ggtgaagaag
    48181 gatgcattat ggcatcttct tcttctagca tgagtaaaat aaaagatttg accaaagaag
    48241 aaatattaag aatagaaaac gacattaaag aaagaaattt taaaattcaa gattttaaaa
    48301 tggaaagaat taaaaagaaa tatgatttca gtagatatat tggaaaattt acaaaaaagg
    48361 aagataagta aatggaacta ttatcaaaat tacctttggc tgatgtacca tatacaagtt
    48421 caatgcaatt gtttttattt ctaactgcat taatgttttt accatttgta atgttcatga
    48481 tgacaagctt tgtcagaata gttataagtt tatcattttt gaaatcagca ttaggagcac
    48541 agcaagcaat tccaagtcaa attttggtag ggcttgcaat agtattgaca atatttataa
    48601 tgagaccagt attaaatgag ataaacgaaa aggcattaca accatatatg aaagaagaag
    48661 ttactatgga agaagctatg aaggaagcag aaggacctat taaagaattt ttattaaagc
    48721 aaaccagaca gacagattta gatttatttg tagagcaagc tggtttaaaa gaaaagaaat
    48781 taactcgtga aaatatacca ttatctgttg tagttccagc atttgctata agtgaactaa
    48841 aaactgcttt tcaaataggt tttttaatat atataccatt tttgataata gaccttgttg
    48901 tggcaagtgt tttgatgtct atgggtatgt ttatgttacc tccagttatg atatcattac
    48961 catttaaatt attattattt gtaatggtgg atggatggaa cttgatagta aaaacattga
    49021 tattgggatt tggataggag aaaaatagta tgagtgaatt tatgataatg agtgttctta
    49081 aaaatggcat gtttacagga gctatgattg cagctccagt gctaattgtt tctcttgtag
    49141 tggggctttt aataagtgta tttcaagcca caactcaaat ccaagagcaa actttgactt
    49201 ttgtaccaaa actaatagca attcttttag tagtagctat tggtggaagc tggatgttac
    49261 atactcttat tagctttact tcggagatat tcaatatgat gtctctcata actaaataag
    49321 taggatatat atgatttttg tatttattag aatagtagca ttctttggaa cattaaggat
    49381 actatttcca gctggtacac ctgctttatt taagtcgctc tttgcaatca ttatatcaat
    49441 cttgatttca agtactatgc aaattgaata tgcaacaaat atagacaaca ttgtattgtt
    49501 taccttatat ggtataaatg agaccataac tgggattatg cttggatata taaccaatct
    49561 atgtttttat agtatcagaa tggctggttc tatgatggac caacagatgg gattatcaat
    49621 gattaatatg tttgacccaa attcaatgac tcagacaact ttaatcaata atcttatgaa
    49681 ctggacagca cttatgatat tttttagcat ggatggtcat catgttttaa ttagaggcat
    49741 aaggtatagt tttgagttaa ttccaatagg gaaatcattc atagataata atatagatta
    49801 tataataaac atatttgtgc agtgcttttt aactggattc aaaatagcaa tacctattgt
    49861 actttgtctg cttatggcag attttatatt agggcttata tcaagaagtg tacctcaact
    49921 aaatgtaatg ataattggta tgccattaaa aatactggtt ggtattgcat tatttatcat
    49981 atcaatacct ttaattgtca atcaaataag tcatttatta agtcaaatac caaagatgta
    50041 tgagggaact tttgcactag ctcctatgtt ttttatgggt tcaacagata aaacagaaga
    50101 agctactcca aagaaaaagg gagaacaaag aaaaaaaggt aatatagcaa agagtaaaga
    50161 attacccatt gcaatgactc tacttgcatt cactttatta gtacctacat tattttcata
    50221 tgtagtgaat acacttaaat cttctcttaa ttattttttg agtctagatt tttatatgaa
    50281 cataaattat tcaagtatag agaaattagt tatagcagga ttaatggatt tttttaagat
    50341 atttttacca atagctattc cattcttagt tttaggtata gtcgcaaacc tatttcaagt
    50401 aggaatactg tttactggtg aaactttaaa acccaattta tcaaaattaa atccaattag
    50461 tggattcaaa aacatgtttt ctatgagaag tttgtcaacc ttaataaaag atacagcgat
    50521 tatatctatt ttggcttaca taggatatac attcttccaa gataattatt tagatatatt
    50581 aaagttaggt aacatatacc tgccaacttt aatgtacaca gttaaagatt tagtatattc
    50641 aatattaagt aagatttgtg tagctatgat agttatagca gttgcagatt atgtttatca
    50701 aagatacagt cataaaaagc aacttagaat gacaaaacaa gaagttaaag atgaatataa
    50761 aaactcagaa ggagacccag aaataaaagc taagataaaa caaaaacaaa gacaaatttc
    50821 gtctcaaaga accatgcaag cagtaccaag tgctactgta atagtaacca atcccaccca
    50881 cttatctata gcagtgagat atgaaaaagg gaaagaccaa gctccagtag tggtggcaaa
    50941 aggtgcagat tatttagctt ttaaaataag ggaaattgca aagggaaatg acatacctat
    51001 aatagaaaat aaacctattg ctaggttgtt atataagcaa gtagaaatag accaagaaat
    51061 tccagaggac atgtatcaag cttttgcaga gatattagta gcagtatata aaatcaaaaa
    51121 tagatacaaa gtccccaaaa ggtaggataa gtaatgaata agtttaattt aaaggaaaat
    51181 atggacgtaa tagtggcatt tggtgttgta gggattgttc ttatgatgat agtgccgtta
    51241 cctacattta tattagatat tttactggca attaacataa gtttatctgt gctaatttta
    51301 ctaatgacac tatttacaac aaatgtactt gatttatcta tatttccaac agtgttactt
    51361 gtgacaacac tatttagatt gggattaaat ctttcatcaa ctagattgat tttaacacag
    51421 ggatatgcag gtgaagttat agaggcattt ggaagttttg ttgtaggtgg aaattacgta
    51481 gtaggaataa ttatattctt aattatatta attgttcaat ttgttgttat aaccaatggt
    51541 tctggaagag tatctgaagt aacagctaga tttacgttag atgccatgcc aggtaagcag
    51601 atgagtatag atgcagattt aaatgcagga gttatagatg acaaaactgc tagaaaaaga
    51661 agaaaagagc ttcagcaaga agcagatttt tatggctcta tggatggagc aaataagttt
    51721 gtaaagggag atgccatagc aggtcttata gtaacagcaa taaacataat aggtggaatt
    51781 gtaataggag ctgtaatgct gaatatgact cttatgggtg cagcacagac atatgtaaga
    51841 cttactatag gggatggtct tgtaagccag atacctgctc tattaatatc tacatcagca
    51901 ggtataatcg taacaagagc ttctactgat gaaaactttg gtggattagt aggtaaacag
    51961 ttaacagcaa taccaaaagc agtaatgatg actggctgtg ttctaatcgt acttggtatg
    52021 atgcctggat tacctaaacc agcatttttt tcactaggta taggtgctat agcagcaggt
    52081 tactttttag gaagagaaaa gaaagatact ttagaagact tggagtttga aaaacctgat
    52141 atggtaaatc cagataaaaa tgaagtagaa gatgtaacaa acttaataaa tgtagaatca
    52201 atagaagtag aaattggata tggtttaata tctttagctg atgaatcagc aggaggagat
    52261 ttacttcaga gaatagcatc aataagaaga caatgtgcaa tagacatggg tatagtcgta
    52321 caacctataa gaataagaga taatttacag ttaaatccaa accaatacaa tataaaaata
    52381 aaagggaatg tagttggtgg gtatgagata atgcctaata tggttttatg tatggatcct
    52441 atgaatcaag gatttagtat atttggaata aagacaattg agcctacttt tggaatagag
    52501 gcactttgga tagaaagtga caaggtagaa gatgcagagc taaagggtta tacagttgta
    52561 gattcatcca caatacttat aactcactta ctagatataa taaaatcaaa tgcacacgaa
    52621 ttactgggaa gacaagaagt taagactatt atagatgcag caagagaaaa ttatagtgct
    52681 gtagtagatg aattgatacc agatttaatg acacttggtg aaattcaaaa agtacttaaa
    52741 aatctgttaa gagaaaaagt aaatataaag gatagagtta caatacttga gacacttgca
    52801 gaccagtcta gaaatacaaa agatatagag cttcttactg aatatgtaag aatagctttg
    52861 gctagaagta tttgtaataa tttagttgat gaagataagg caattgtagt ggctacattg
    52921 tctccagaaa ctgaagaatt ggtatctaat aatttacaaa ggtctgtaaa tggaacttat
    52981 ccagcagttg acccagaaaa tacaaataaa atatttgaga gtatacaaga agtaatgaac
    53041 aatgtatatt tcaatcataa tataccagta atagtagtat caccaaagat aagagctcca
    53101 tttagaaaat tagttgaaat tgtatatcct aatttgactg tattatcatt aaatgaaata
    53161 cctaacgata ttaaaatcaa agcagaaggt gtggtgagca tatatgatga cgaaaaagta
    53221 tacagctaac acaattcaag atgctatgaa tctagccaaa atggaattgg gtgacaatat
    53281 tacattaata gataaaaaag aggtaagaaa atctggaata agaggcattt tttcaaagaa
    53341 agatattgaa ttgactattg gatgggaaaa gaaagataat ttagaacaaa aagatttaaa
    53401 acgagagata gaacaactaa agtcaattat aaataatatg gggtttgata ataaaaatga
    53461 taatgatata gataaaatat gcaagaattt actaaactta gatttaaatc aagaaattgt
    53521 agaatttata aaagctgatt tacaagaaat gaagttcaat ggaattgata caagtaaaaa
    53581 tttagtagaa agtctaaaga aaaagattaa aatagaaaat caagcaataa atggtaaaat
    53641 tgctttagtt ggaccaccag gagtaggtaa aacgactaca atagcaaagt tggctgccaa
    53701 attagttttt gaggaaaata aaaaagtagg agtaataact atagatacat acaggattgg
    53761 tgctgtagaa cagttaaaga tatatactga tataatgaat attcctttta aaggggtaat
    53821 aagccctgat gagatggaat tggcacttga tgagatgaaa gattgtgatg ttgtattaat
    53881 agatacaaca ggtagaggct ataaaaactc tatgcaaata ttagaaatca aaaatcttat
    53941 agataaagct gaaactgata atatacattt agttttgaac tgtacaacaa gagaaagtga
    54001 tacaaaagca attatagatt catatagaaa tgtgaatttt aaaagcttaa taattacaaa
    54061 gttagatgaa acaataacat atggctcaat atttaacata atgaattatg cacaaaaacc
    54121 aatatcatat attacaacag gacaaaatgt tcctgatgat attataaaac ctaatgaaga
    54181 taagattata agattactat taggagttga aagtatatga tagaccaagc tcaaattttg
    54241 agaaaaatgg caatggaaaa aagaggttct gatgagatta tagtagagaa tgagaatatg
    54301 cctaagataa ttacaatagc ttctggtaaa ggcggcgttg gaaagagtaa tctcgctaca
    54361 aatttgtcta tatgcttaac caaacttgat aaaaaagttc ttatattaga tgcagatata
    54421 ggaatgtcta atatagatat aataatgggg gtaaatgtaa aagggactat aattgatgta
    54481 attaatggcg aaaaaaatat agaagatata atttcacaga ctaagtatgg tgtaaatata
    54541 atatctggag gttctgccct taatcatata gaagacttta cagaagctca aagaaataag
    54601 tttatacata gcatagaaca gattcacaat gtggatttta taataattga tacaggagct
    54661 ggtatgagca aaagcctatt gtcatttata tactgtagta ctgaattttt cttaataaca
    54721 actccagaac cgacatcatt gactgacgca tatagtttat taaaagctat aagtaatttt
    54781 ggcataaaga aaagtgcaaa tataataatc aatagggtaa ttgatttaga agaagcgaaa
    54841 tctacataca aaagaataaa aatggttgtg gacaaatttc taaaccttaa tctggaattg
    54901 tatggatata taatagatga caaaaaagta ggtgtatgtg taaaaaaaca gataccattc
    54961 attttagaat atcctacaag tattgcatca aaatgtatta tagctatagc aaagagaatg
    55021 gtaagtcagt caagagaaga tattgttgat aataaaagtt ttctaagaaa gatgtttagt
    55081 atatttaaaa gctaaggagg cgtagttaat gaatagagaa gaattaataa aagagaatat
    55141 gcctcttgta aagagtatag caagaaaatt ttttatacca gggaaaggtt ttgaatatca
    55201 agacttagta aatagtggag tcattggatt gattgatgca ataaataaat ttgatactga
    55261 aaaaggagca aagttttcta gttactctta tataaagata aaatctgcta tattagatga
    55321 aattagaaat caaagtccaa tttctaaaca taatctaact aaagtaaata agtataatag
    55381 agtggtagaa aagttgcaat ctaaattatt aagagagcca acttcatatg aaattgcaaa
    55441 agaattaaag gtttcagaaa aagaacttca tgatatcgaa agtaatatag atatgttaaa
    55501 tattgtatca ttaaactatg taatctttga agatacaaat gaaactgtac aagatgtaat
    55561 aagtgataga gaagaggaag ctccagaaaa tataatagaa gaagaagaaa agttagagat
    55621 tctatccaaa gcaattagta atttaaatga aagagaaaaa ttaatacttt cactgtacta
    55681 ttatgaagat ttaaatctaa aggagatagg gaaagtgcta ggtgtttcag aatcaagagt
    55741 ttcacaactc cacagaaaat caataagaaa cttaagaaac aagataaaag aacttaaata
    55801 ttctatatag atggtgattg ataagatgat acaaattata ttaatagtag tgtctatttt
    55861 aataatagta actatagtta taaatttaca gaaggaatca aaaaataaag ataacagata
    55921 ctttgataaa atatttgagc aagaatatga agcaaatgat agattaataa ttgcagaaaa
    55981 aagaagattc tttttagaca aagtacttgc agaaaaaaga attaatagtc atataaaagt
    56041 ttatgaaaat tataacctaa taaaggctaa tgaagaaaga aaggaaaaca tatttataga
    56101 atcaaaaaag gaagtaagac aagaaaatga gttatctctt aaagtgaaac atcttataag
    56161 ttcaggttat gatgatgtgg aaatatgtaa gatgctagat attggaaagg gggaactgtt
    56221 attaataagg agttgttaca aaatataagg atattattat gtgataaaaa agtattctta
    56281 ggaataggta ttggtatatt aatatcaact ttatgtataa tgctttttag taataatgat
    56341 atgagtaagg cagagataga aaaaaaggcc agagcgttag gcatgaaata tacaagtgag
    56401 atgaaagttt tagaatagat gatactgttt agacatttac aaaaaaggag gtcataatgt
    56461 gataagagga ttatatacag cagtttctgc tatgataact aatcaacaaa agcaaaatgt
    56521 tgttgtcaat aacctagcta atatggatac aaatggatat aaaagtaaac aacttttgac
    56581 taaaagtttt gatgaattaa agttggaggg ttacaataac tacgcaaatg gaactaaaca
    56641 aaagcagatt ataggcgata taagtccagg tatttctatg gatgaaacta tcacaaattt
    56701 taatcaagga cctataaaaa ctactgataa taaaatggat gtagctattc aaggtaaggg
    56761 attttttcta gttagtgatg cagctggaaa tcaattttac actagaaatg ggaacttcag
    56821 aacagacaat caaggtgacc ttataacaag tgaagggtat tatgtacttg ggacgaatac
    56881 tgctactggt gctgtaggtc atataaatgt aggaaatcaa aaatttgaag tggcaaaaga
    56941 taatacaata tcactagacg gaaatgctat gtataaattt aatattgtag attttaataa
    57001 ctacaataac ttaaaaaaag aaggagataa cttatattct ggaggaggag ctatgaaagt
    57061 aaataatgca cttacaatac aaagtgcgat agagtcctct aatgtagata tggtatctga
    57121 aattaataat atgatgacaa tatcaagaga atatgaggca aatcaaaaga taattcaggc
    57181 tatggattct aaattagcaa aaatagcaag tgagattggt tcagtaagat aggtaatagg
    57241 aggtgaattt ttatgtacac aatgttttat actagtaaaa cagctttatc agcaaatcaa
    57301 agtaaactag atataatatc taacaatata gcaaattctg attcaacagg atataaaaaa
    57361 ataaatatgg aatattctga tttagtggat gaagtactaa atagaccatc ataccctaca
    57421 aatggtaaag atatatcgac tggaacagga gctaaggcaa cagctcccat gagaaattat
    57481 gaacaaggtg cattggctcc tacagatagt aagagtaatt tggcgataga tggagaagga
    57541 ttctttagag tgataagaaa agatggtaca tatgcatata caagaaatgg tggatttaat
    57601 gtagatgctt taggtaagat tgtagatgat aatggaaata tattagatgt ccaatttgat
    57661 gcaggatata actataataa tacaaatata gatagtacaa acctgacagt ttcaagaaat
    57721 ggagagctat tcgtagataa taaaaaaata ggaaaaatta acttatatca acctatagga
    57781 actcaaaatt ttatatctga aggagatagt ttatttgtcg caactgatgc agcacagatt
    57841 aaacaagttg agaaagtcga tatagttcaa ggctatagag agaggtcaaa tgtttcttta
    57901 caagaagaat ttgttgactt aatagccact caaagagcat ttcaaatgaa ctcaaaggga
    57961 ataaagacag cagatgaaat gtggcaaata gcgaataatt taagagctaa ataattatgt
    58021 aaggagagga atattcatga atgatatgaa agagtacgat tttggcaaac ctcagagtta
    58081 ttcaagagaa aatttaactt gtttaaattt tttattagct gaattttgta aaaaatataa
    58141 aaactatata atttatgaac ttaagtgtaa ttctagtatg gttgttgata gtatagacca
    58201 agtaaattat cagacttttt tagatagagt aacttcttca tgcgtagtag tacaaaatgc
    58261 aatggaacct gttatgaaaa acttttcttt tagaatagat agaagtgtag ctgatatgtg
    58321 gattgatata acttctggag gaactggagt tgtaaaggat aatgatagag agcttacaga
    58381 acttgataaa aaagtgcttt tacaccttat ggatgattta gtaagaaata tgcacttatt
    58441 tgagggtttt gaaaatatac aagtattgga tacacataca catattaatt tgccacaatt
    58501 atgttctcca actgcaccag tatgcgttgt agatttaaaa gtgctaaatg ataatgagtg
    58561 tattggaagt gtttctcttt gttttccata caatggttta gaggctctgt tagaaagtat
    58621 atctgttata gaattttttg ataatgatgt aggagatgat tcagaggaat ttacccaaaa
    58681 aatctataat agtgtatcta atatagaatt atcagttatt gcagagatag gaaaagtatc
    58741 aattaatgtt gaggatttac ttaaactaga agttggagat gtaatagtaa caaataaaaa
    58801 aataaatgat tatatagata tatttgtaga aaattcaaaa tcatacacag caacaccagg
    58861 atttatatca agtaaaaaag gtgtaaaaat aaatgatgct gttggaaaag aggtgtagtt
    58921 atggacaata gtaatcttat ttcagatgaa gaagcaaaga tgttattaaa tgataaagtt
    58981 gaaagtgaag ttgatggaga tgatgagtta gccttagatg tctctgaagt taatcgaaat
    59041 aatatacaaa ggatacttga ccttaagtta gaattatctg tagttatagg aagaactaag
    59101 atgactctaa gagatatttt aaatttacat aaaggttctc ttattgaact ggatacttta
    59161 gcagaccaag atgtggaaat actaatagat aataaggttt tggcttatgg aaaagtagtg
    59221 gtggtagatt taaattttgg tgtaaagata acaagtatag taagtgaaga agacatggta
    59281 aaatctcttg tttagagata acatcaatta atacttaagg catgaggtgg tacttatttt
    59341 aaacttaaat gatataaaaa aagatatact aaaactcaat aaaaaagact catgttctct
    59401 atataatgaa tctattaata atataaataa taattgtata tcaactggat taaaattttt
    59461 aaaacaagca agagatttaa acccagatga tgttgatata ttaaatctca caggtcttgt
    59521 aaacttacta aaatgtaatt ttgatgatgc tgtagaaagt ttctataaga gttcactttg
    59581 tgaaaaaact gctctgtgtg aaaagtatat aaacatgctt acatctgagg agtttttaat
    59641 tttctttgaa aagtacaatc aacttataag gttaataaat aaaggaatgt ataaagaagc
    59701 tatacaaggg tttaaactca taactgacca atactgtgat ttaatagaac cttatgagtt
    59761 gcttgcctta ctgtatgaca aggaagaaga atatataaaa tttgatgaat gtttagaagt
    59821 attgaagatt attgataagg aaaattattt attaaatcaa gaaaataaca cattagatat
    59881 gtataatagt gctaatatac aaactaaaag taatataaaa gtagatgatt ataagaatga
    59941 gttcttacat aaaagtagta gtaaccataa aattaatgat tataatgatg taactaaaga
    60001 aaagagttca agcaaaaacc agaaaagttc agattctaat aagaaaaaaa gtattaaaaa
    60061 aaataagtac ctatatatag ccataatatt aggaatatta ttagtaggac aaagtatata
    60121 taattcttat aagatgaata atttaactga taaaatatct aaaaataatg aaactaaaaa
    60181 tgaagagtca atagattcta acttgactaa gaaagaaaat aatatagaag aaaatgaaga
    60241 tatgagtaag gcttctaata taaataagag taataaaaac attaataaag aggatgatgt
    60301 aaaatctaaa aaaaatactg tagaaaatga agatgataaa gattctgact tatttacaga
    60361 ggatgaattg atgtctaagg cgaatacttt aaaatcagaa aagaaaacta aatcttctat
    60421 acaactatat aagaaagttg cagatatagg tgaaaataaa gggaatacat ctgaagcaac
    60481 ttatcaagtt gctattctca gtgaaaagtt aaaagactat aaaactgctg aagaatatta
    60541 taaaatgtat gtagaaaatt actcagaaaa agatgcttat tttgatgaat cttattataa
    60601 tttaggtatg atgtactata acaatggtga tttaaaaaat tctaaattga cattaaagaa
    60661 gttggttaat aaggtaccaa atagtatgta taataattca aaagtaaaag aaattttaaa
    60721 agaagaatag ttaaattaga taagaaaaat aaattatttt attttaaaat aatatgatta
    60781 taatatattg acataccatt ttaaaaaatt taaaatatta attataaaat gaggagggat
    60841 aaaaaatgga atttgaaaaa gggagtattt caattcatac agaaaatatt tttcctataa
    60901 ttaaaaaatg gttatactct gataaagaca tatttataag agagcttata agtaatggat
    60961 gtgatgcagt aagtaaacat aaaagacttg tatcactagg agaaatatca gaaaataagt
    61021 catcagatta taaaattaca gtatctgtaa ataaaggaga aggtacactt aaatttattg
    61081 acaatggaat tggtatgact gaagaagaga taaaaaaata cataaatcaa gttgcttttt
    61141 caggtgctga agatttcttt aataaatata aggataaaat ggaagagtca aatgatataa
    61201 taggtcattt tggattagga ttttattctg catttatggt gtctaaaaag gtacaaatag
    61261 atacactttc atatacagaa ggagcgactc ctgtaagatg gatatctgaa ggtggtacag
    61321 aatacgaaat atctgaatca gatgctagaa atgatagagg aactactata acactattta
    61381 ttgatgatga tagtaaagaa tttttagatg aatttactgt aagaggaata ataaataaat
    61441 actgttcttt cttaccagta gaaatatatt tagaagatgt tgaaagatta gaaagagaag
    61501 ctaaagaagc agaagaaaaa gctaaaaaac aaaaggaaga tggaaaagaa gagattgtag
    61561 atgcaaaagt aattgagcct ttaaacgaca ctaatcctct atggttaaaa tctccaaaag
    61621 actgtactga tgaggagtat aaagagtttt atagaaaggt attcaatgta tttgatgaac
    61681 ctttattttg gatacattta aatgtagatt atcctttcaa tttaaagggt atactttact
    61741 tccctaaatt aaaaaatgag tttgaactga ctgaaggaaa agtaaaatta tacaacaatc
    61801 aagtgtttgt ggctgacaat ataaaagaag ttataccaga atttttactt ttattaaaag
    61861 gagttataga ctgcccagac ttaccattaa atgtatcaag aagtttctta caaaatgaca
    61921 gggatgtaag caaaatttct aaacatataa ttaaaaaagt agctgataag ttaaaatctc
    61981 tttgtaaaaa tgaaagagaa gaatacaata agttttggga tgatatacaa atatttatta
    62041 aatatggttg cttaaaagat gaaagtttct atgagaaggt aaaagaatgt atattattca
    62101 aaactattga tgatgagtat ataacattac aagattattt agaaaagtgt aaggacaaac
    62161 atgaaaataa ggtattttat gtaagtgata aagaacagca atctcaatac attaaactat
    62221 ttaaagaata cgacttaagt gctgttgttt taaactcatc aatagatact cattttattt
    62281 catttatgga atataaagag aatggagtta agtttaatag aatagatgca gacctttctg
    62341 atgtattaaa agataaaaat gaaaataaag atagtgaaga aaacaaagaa gagatagcta
    62401 agatagaagg tctgtttaaa gaagctgttg gagaaagagt gaagaattat tctgtagaag
    62461 gattgaaaaa tgaagacact ccagctatgg tgttagtatc agaacaatct attagaatgg
    62521 cagaaatgca gtcaagattt gctggaatgg atttaggaat gaattttgaa gaagaaaaaa
    62581 ctcttgtaat aaatgagaac agtccaataa tcaaaaaatt ggtttctctt aaagatgatg
    62641 aggaaaagaa agataagatt acgcttattt gtaaccagat agcagattta gctttacttt
    62701 ctaataaaga attaaagcca gatgagttag attcatttgt acaaagaagt aataagctta
    62761 tgagtctttt gatagaatta taagattgaa tttgaataca ttgaagcaaa tttataaaaa
    62821 gtattcaata tcagtgataa gaaagaagtg tctcaaaatg aacttttcag ttcatgaggc
    62881 gcttttttta tgtgaaatta aatttatttt aatcatttca acaatgaaaa atatatttat
    62941 ctctattttg agacagaccg ttctttattt ttttatcttt ggttaatttt taaatattta
    63001 agtaggttct aataaaaaac tctatttagt taaatatagt taacttgata tataataaga
    63061 taatacatat aattaagtaa aagattgttt tttagtttat gtttaaatac ataattacac
    63121 tttgttaaaa acataaaatt attactaaag tagaaatagg aggttttagc atgaatttta
    63181 attataattt gccagtaaat atcttatttg ggagcggcag aattaatgat gtgggaaaag
    63241 aagtatctaa atatggcaaa aaagtattga tagtaacagg taaaaatagt acaaagaaaa
    63301 ctggattact tgataaaaca atagatttac taaaagactc taaaatagaa tatgaagttt
    63361 ttgatagagt agaacaaaat cctcttataa ctactatata ttcaggagta gaaattataa
    63421 aatcaactgg atgtgattgt gttttaggtc ttggtggtgg aagcattatg gatgctgcca
    63481 agagtatagc tttttcttat aaaaacccag gagatttaaa tgattatata tttggaatta
    63541 gacaaggtga agaagcttta cctataatct tagttccaac aacatcaggt acaggtagtg
    63601 aaggaaattg ttttgcagta cttacaaatt cagaaactaa agataaaaaa tctataaaga
    63661 agaatagtat gtatgcaaaa gcatcaataa ttgacccaga acttatggtt acaatgccaa
    63721 aacatataat tgcatcagtt ggatttgatg cacttgccca taatatggaa gcatatcttt
    63781 ctaatggaag aaaccctctt gcagatgtac aagctattta tgggattgaa cttatatcag
    63841 aaaacctaat aaaggtatat aatgatgtta atgatttaga agcatgggaa aaaataacac
    63901 ttgcaagtac tattggagga atgacgattg gtacagcggg tacagccttg ccacatggaa
    63961 tggagcatcc tgtaagtggt cttagagatg taatgcatgg aaaaggactt gcagctttga
    64021 ctcctactat tatagaaagt tcatgggata gtgatattga aagatttggt aatatttcaa
    64081 aggtattagg aggaacttgt gcagaagatt gttcagatgc aattagaaat ttccttaaaa
    64141 aaatagattt agatataaag ctaagtgatt taggtattga aaaatcagat atagattggt
    64201 taactgataa ttgtatgaaa atatcagttg caaatattaa gagacatcca aagtatttta
    64261 ataaggaaca aataaaggaa atgtaccata agtctttata atttgacttt acatatttag
    64321 ttttatataa aaattataaa ttaataataa aaaattataa g
//
LOCUS       DAYASV010000006        64160 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYASV010000006 DAYASV010000000
VERSION     DAYASV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.006"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            123..1319
                     /locus_tag="ACGI68_000418"
     CDS             123..1319
                     /locus_tag="ACGI68_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFH0136876.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1412..1936
                     /locus_tag="ACGI68_000419"
     CDS             1412..1936
                     /locus_tag="ACGI68_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFH0136877.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1961..2137
                     /gene="rpmF"
                     /locus_tag="ACGI68_000420"
     CDS             1961..2137
                     /gene="rpmF"
                     /locus_tag="ACGI68_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFH0136878.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2344..2901
                     /gene="fapR"
                     /locus_tag="ACGI68_000421"
     CDS             2344..2901
                     /gene="fapR"
                     /locus_tag="ACGI68_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HFH0136879.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2912..3934
                     /gene="plsX"
                     /locus_tag="ACGI68_000422"
     CDS             2912..3934
                     /gene="plsX"
                     /locus_tag="ACGI68_000422"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFH0136880.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3960..4946
                     /locus_tag="ACGI68_000423"
     CDS             3960..4946
                     /locus_tag="ACGI68_000423"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFH0136881.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5055..5984
                     /gene="fabK"
                     /locus_tag="ACGI68_000424"
     CDS             5055..5984
                     /gene="fabK"
                     /locus_tag="ACGI68_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HFH0136882.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            6000..6950
                     /gene="fabD"
                     /locus_tag="ACGI68_000425"
     CDS             6000..6950
                     /gene="fabD"
                     /locus_tag="ACGI68_000425"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFH0136883.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNAVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPAGVGAMAAVLGLDRNVLENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6950..7699
                     /gene="fabG"
                     /locus_tag="ACGI68_000426"
     CDS             6950..7699
                     /gene="fabG"
                     /locus_tag="ACGI68_000426"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFH0136884.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7753..7977
                     /gene="acpP"
                     /locus_tag="ACGI68_000427"
     CDS             7753..7977
                     /gene="acpP"
                     /locus_tag="ACGI68_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0136885.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            8006..9244
                     /gene="fabF"
                     /locus_tag="ACGI68_000428"
     CDS             8006..9244
                     /gene="fabF"
                     /locus_tag="ACGI68_000428"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFH0136886.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADLMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9470..11188
                     /locus_tag="ACGI68_000429"
     CDS             9470..11188
                     /locus_tag="ACGI68_000429"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0136887.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKYDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11219..13219
                     /locus_tag="ACGI68_000430"
     CDS             11219..13219
                     /locus_tag="ACGI68_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0136888.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13570..13815
                     /locus_tag="ACGI68_000431"
     CDS             13570..13815
                     /locus_tag="ACGI68_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFH0136889.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13850..14575
                     /locus_tag="ACGI68_000432"
     CDS             13850..14575
                     /locus_tag="ACGI68_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFH0136890.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14597..15910
                     /locus_tag="ACGI68_000433"
     CDS             14597..15910
                     /locus_tag="ACGI68_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0136891.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKIYGENLKKIV"
     gene            16116..16655
                     /locus_tag="ACGI68_000434"
     CDS             16116..16655
                     /locus_tag="ACGI68_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0136892.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16744..18729
                     /locus_tag="ACGI68_000435"
     CDS             16744..18729
                     /locus_tag="ACGI68_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HFH0136893.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGIKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18953..19921
                     /gene="spoIIIAA"
                     /locus_tag="ACGI68_000436"
     CDS             18953..19921
                     /gene="spoIIIAA"
                     /locus_tag="ACGI68_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HFH0136894.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19911..20432
                     /locus_tag="ACGI68_000437"
     CDS             19911..20432
                     /locus_tag="ACGI68_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HFH0136895.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20449..20646
                     /gene="spoIIIAC"
                     /locus_tag="ACGI68_000438"
     CDS             20449..20646
                     /gene="spoIIIAC"
                     /locus_tag="ACGI68_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HFH0136896.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20671..21048
                     /gene="spoIIIAD"
                     /locus_tag="ACGI68_000439"
     CDS             20671..21048
                     /gene="spoIIIAD"
                     /locus_tag="ACGI68_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HFH0136897.1"
                     /translation="MQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVMFKL
                     NFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEFGGK
                     IIVMSMSFPILLSIVEMVVNIIP"
     gene            21133..22338
                     /gene="spoIIIAE"
                     /locus_tag="ACGI68_000440"
     CDS             21133..22338
                     /gene="spoIIIAE"
                     /locus_tag="ACGI68_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HFH0136898.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22398..23015
                     /locus_tag="ACGI68_000441"
     CDS             22398..23015
                     /locus_tag="ACGI68_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HFH0136899.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            23027..23581
                     /locus_tag="ACGI68_000442"
     CDS             23027..23581
                     /locus_tag="ACGI68_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HFH0136900.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23608..24297
                     /locus_tag="ACGI68_000443"
     CDS             23608..24297
                     /locus_tag="ACGI68_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HFH0136901.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDESDSSKTEDSSNVDKQDGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24407..24769
                     /locus_tag="ACGI68_000444"
     CDS             24407..24769
                     /locus_tag="ACGI68_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFH0136902.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24854..25360
                     /gene="nusB"
                     /locus_tag="ACGI68_000445"
     CDS             24854..25360
                     /gene="nusB"
                     /locus_tag="ACGI68_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFH0136903.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25344..26414
                     /locus_tag="ACGI68_000446"
     CDS             25344..26414
                     /locus_tag="ACGI68_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HFH0136904.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26411..27616
                     /gene="xseA"
                     /locus_tag="ACGI68_000447"
     CDS             26411..27616
                     /gene="xseA"
                     /locus_tag="ACGI68_000447"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFH0136905.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27618..27833
                     /gene="xseB"
                     /locus_tag="ACGI68_000448"
     CDS             27618..27833
                     /gene="xseB"
                     /locus_tag="ACGI68_000448"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFH0136906.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27836..28723
                     /locus_tag="ACGI68_000449"
     CDS             27836..28723
                     /locus_tag="ACGI68_000449"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFH0136907.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIGKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28753..29190
                     /locus_tag="ACGI68_000450"
     CDS             28753..29190
                     /locus_tag="ACGI68_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HFH0136908.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29224..31089
                     /gene="dxs"
                     /locus_tag="ACGI68_000451"
     CDS             29224..31089
                     /gene="dxs"
                     /locus_tag="ACGI68_000451"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFH0136909.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31222..32034
                     /locus_tag="ACGI68_000452"
     CDS             31222..32034
                     /locus_tag="ACGI68_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HFH0136910.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32054..33733
                     /gene="recN"
                     /locus_tag="ACGI68_000453"
     CDS             32054..33733
                     /gene="recN"
                     /locus_tag="ACGI68_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFH0136911.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33947..34813
                     /locus_tag="ACGI68_000454"
     CDS             33947..34813
                     /locus_tag="ACGI68_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136912.1"
                     /translation="MNKKDTMHLIFTIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECVIDVDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34906..35397)
                     /locus_tag="ACGI68_000455"
     CDS             complement(34906..35397)
                     /locus_tag="ACGI68_000455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0136913.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35458..35898)
                     /locus_tag="ACGI68_000456"
     CDS             complement(35458..35898)
                     /locus_tag="ACGI68_000456"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0136914.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36059..37117
                     /locus_tag="ACGI68_000457"
     CDS             36059..37117
                     /locus_tag="ACGI68_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFH0136915.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37259..38083
                     /gene="spo0A"
                     /locus_tag="ACGI68_000458"
     CDS             37259..38083
                     /gene="spo0A"
                     /locus_tag="ACGI68_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HFH0136916.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38496..39617
                     /gene="steA"
                     /locus_tag="ACGI68_000459"
     CDS             38496..39617
                     /gene="steA"
                     /locus_tag="ACGI68_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HFH0136917.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39635..40510
                     /locus_tag="ACGI68_000460"
     CDS             39635..40510
                     /locus_tag="ACGI68_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HFH0136918.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40534..41226
                     /locus_tag="ACGI68_000461"
     CDS             40534..41226
                     /locus_tag="ACGI68_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0136919.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41234..42058
                     /locus_tag="ACGI68_000462"
     CDS             41234..42058
                     /locus_tag="ACGI68_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HFH0136920.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKNLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIVFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42063..43130
                     /locus_tag="ACGI68_000463"
     CDS             42063..43130
                     /locus_tag="ACGI68_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HFH0136921.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQKAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43132..43668
                     /locus_tag="ACGI68_000464"
     CDS             43132..43668
                     /locus_tag="ACGI68_000464"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0136922.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43736..44341
                     /locus_tag="ACGI68_000465"
     CDS             43736..44341
                     /locus_tag="ACGI68_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HFH0136923.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKSR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44353..45267
                     /locus_tag="ACGI68_000466"
     CDS             44353..45267
                     /locus_tag="ACGI68_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0136924.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAAEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45309..46481
                     /locus_tag="ACGI68_000467"
     CDS             45309..46481
                     /locus_tag="ACGI68_000467"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFH0136925.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIEGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46528..47340
                     /locus_tag="ACGI68_000468"
     CDS             46528..47340
                     /locus_tag="ACGI68_000468"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFH0136926.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47544..48869
                     /locus_tag="ACGI68_000469"
     CDS             47544..48869
                     /locus_tag="ACGI68_000469"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFH0136927.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49082..50161
                     /gene="mltG"
                     /locus_tag="ACGI68_000470"
     CDS             49082..50161
                     /gene="mltG"
                     /locus_tag="ACGI68_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFH0136928.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFSYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50333..51007
                     /locus_tag="ACGI68_000471"
     CDS             50333..51007
                     /locus_tag="ACGI68_000471"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFH0136929.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRKYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50994..52241
                     /locus_tag="ACGI68_000472"
     CDS             50994..52241
                     /locus_tag="ACGI68_000472"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFH0136930.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEIYEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52313..53983
                     /locus_tag="ACGI68_000473"
     CDS             52313..53983
                     /locus_tag="ACGI68_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFH0136931.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDIDKSMKKELEKNGIMVEEKTMRYAKDGL
                     LSHTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKT
                     VDNKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQND
                     ISKSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNT
                     NEILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGL
                     DEEKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNM
                     NTIKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLN
                     KKAIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54080..54364)
                     /locus_tag="ACGI68_000474"
     CDS             complement(54080..54364)
                     /locus_tag="ACGI68_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0136932.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54387..55904)
                     /locus_tag="ACGI68_000475"
     CDS             complement(54387..55904)
                     /locus_tag="ACGI68_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0136933.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKAYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(56029..56850)
                     /locus_tag="ACGI68_000476"
     CDS             complement(56029..56850)
                     /locus_tag="ACGI68_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0136934.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            57041..58468
                     /locus_tag="ACGI68_000477"
     CDS             57041..58468
                     /locus_tag="ACGI68_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
                     /protein_id="HFH0136935.1"
                     /translation="MKIKKLLTIGLSLSIFIASCPISVNALDKIESIKGADKYETAGI
                     IADKQNYTTAILINADSTMADGLSASGLAGAINAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKIVKDTNSTR
                     LGGTDRYDTNKKIINKFYSGVKEFYIASGTDLVYALVGSTVAKNNAIVLVDNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGREYTVEDAANAVKKLLGVKKFYYE
                     PNYSEDEDLIFLLDKNFDFSGKEYYVFAYIKQGAEGDVRYCVAKNDMSKVYTYDVNGN
                     MKVCNPDDYKNKGSITEEQAKQIALKECAKRHKVSINNLVVDMIDFRDDIYSGNVYAV
                     KINSKNSNGTFGWFFIDIETGKVYN"
     gene            58618..58770
                     /locus_tag="ACGI68_000478"
     CDS             58618..58770
                     /locus_tag="ACGI68_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136936.1"
                     /translation="MDNFLFNVLASLTASVVVYLISKLFKKAKSHSRTKSDLRVEFKF
                     IFKSKK"
     repeat_region   59424..59716
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=59424..59452
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(60003..60227)
                     /locus_tag="ACGI68_000479"
     CDS             complement(60003..60227)
                     /locus_tag="ACGI68_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0136937.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRD
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            60938..61093
                     /locus_tag="ACGI68_000480"
     CDS             60938..61093
                     /locus_tag="ACGI68_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFH0136938.1"
                     /translation="MALDVKIEVKIIKDVVILNITHWKKETSLLTVKIARSQGDLSYV
                     KYFANES"
     gene            61372..61590
                     /locus_tag="ACGI68_000481"
     CDS             61372..61590
                     /locus_tag="ACGI68_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136939.1"
                     /translation="MKILQQFKPYKYDKEKNIEYDEKIIANQNTEVYIVYPRISDEEN
                     RRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            61898..62023
                     /locus_tag="ACGI68_000482"
     CDS             61898..62023
                     /locus_tag="ACGI68_000482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136940.1"
                     /translation="MRFEMVEVNIKIKGEVSEIINSIRGIFNSFDEELILDIKSE"
     gene            complement(62800..63183)
                     /locus_tag="ACGI68_000483"
     CDS             complement(62800..63183)
                     /locus_tag="ACGI68_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136941.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63310..63654
                     /locus_tag="ACGI68_000484"
     CDS             63310..63654
                     /locus_tag="ACGI68_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136942.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVFLILIQ"
     gene            63837..64052
                     /locus_tag="ACGI68_000485"
     CDS             63837..64052
                     /locus_tag="ACGI68_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136943.1"
                     /translation="MHINQTGFISNELGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFELIATLVRGGSSWAFNFIVQP"
BASE COUNT        25707 a         6654 c        11501 g        20298 t
ORIGIN      
        1 tttaatgtat acaataatat atctataaca aaataataaa agatagagtt ttatttagac
       61 atttattaca aatcaaaatt tattacatat tatttataaa aaaatctaag gaggaaataa
      121 taatgaaaat attagtatta aactgtggga gttcttcatt aaaatatcaa ttaatagata
      181 tgaataatga agaagtttta tgtataggat tggttgaaag aataggaata gaaggttcta
      241 tattaaaaca tgaaaaagca ggtagagatg ataaatacgt tgttgaacaa ccaatgaagg
      301 accacaaaga tgcaatagca ttagttttag aagctgttgc tcatccagaa tttggtgcag
      361 ttaaagaaat gaaagaaata gatgcagtag gacatagggt tgtacatgct ggagaaaaat
      421 ttgcaacttc agtagtaata actccagaag tagaagaagc tttaaaagaa tgtatagatt
      481 tagctccact tcataatcca gcaaacataa tgggaataga tgcttgtaaa gctatacttc
      541 cagatgtacc aatggtaggt gtatttgata ctgctttcca tcaaacaatg cctaaatctt
      601 catacttata tggtttacct catgagctat atactaaata tggggtaaga agatatggat
      661 tccacggaac ttctcataat tatgtatctc aaagagcagc agagatatta ggaaaagata
      721 taaaagattt aaaaatagta acttgtcact taggaaatgg tgcttctata gctgctgttg
      781 atggtggaaa atgtgtagat acatctatgg gattcactcc attagaagga ttaataatgg
      841 gaactagatg tggagatata gacccagcta tattaccatt cttaatgaga aaagaaggtt
      901 tagatgctga tggattggat aaattaatga ataaagaatc tggagtatat ggaatgactg
      961 gaatttctag tgacttcaga gatatagaag atgcagctaa aaatggagac gaaagagctc
     1021 aagctacatt agaagcgtat gttaaaaaag tacaaaaata tataggcgct tatgctgctg
     1081 aaatgaatgg attggatgtt gtagtattta ctgcaggcgt tggtgaaaat ggaaaggcta
     1141 taagagcaga tattgcttct aacatggagt ttttaggaat gaaattagat aaagaagcta
     1201 atgatgttag aggaaaagaa acagtaatat ctactgctga ttctaaagta aaaatgcttt
     1261 taataccaac taatgaagaa ttaatgatag ctagagatac attaagattg gtaaaataat
     1321 taaattaaac ctatgcctat caagtaaaaa tacttgacaa acaaatgcta gatttgtata
     1381 ataaatttgg tgatatgttg aggtgaactt aatgaaagtt agcatagaaa aaataaatag
     1441 aaaagaaact gacaaaatag acttgaattt ttgtgaaaaa attgatacta ttagttattg
     1501 tgatgagatt tacaaattag tatcacctgt aaatttaaaa gggaaagtgt caaagactaa
     1561 taaaggtttg tatctagata tagatgtcaa ttttacaatt gttgacaatt gttcaagatg
     1621 tcttaaagaa gtagaaatac cactagagta ttctatacaa ggttttttag taaaagaaga
     1681 agattatgat gaagatgagt ttgaggaatt tgatcctttt atattcgatg gtgaagaaat
     1741 cgaccttatt gatataatag aacagacatt agattttaat gtacctcata aagttctttg
     1801 tagtgaaaac tgtaaaggtc tttgtcaagt atgcggggca aacttaaatg aagaagagtg
     1861 ttcttgcagt gaaattacaa acgatgagga atacatagat cctcgttttg ctaaattaaa
     1921 agatttattt aattaaattt tgactaagga ggtggcgtaa atggcagtac caaagcgtaa
     1981 aacgtctaaa tcaaacacaa aaatgagaag agcagctaac tcaaagatgg aagcaacagg
     2041 atttgtaagt tgcccacaat gtcatgagcc aaaattacca catagagtgt gtccagactg
     2101 tggatattat aaaggtaaag aagttgtgtc taagtaatag cttctaaaaa gcatgtagag
     2161 tatactctac atgttttttt tgtacatata aaaaaataag ataatttact agagaaaata
     2221 agtggatttt atgcatatta aaaaatagaa aaataataat ataagtattg aaaatttttg
     2281 tgtgttacta tatactacta ttagtagctg gtactaatta cagctataaa atttggaggg
     2341 aacatgaaaa agaaaagtaa agcccaaaga caaaaagagc ttatagatat gctaaagaca
     2401 gatccttttt atactgatga agaattatca agtctgtttg atgtaagtat acagaccata
     2461 agattagata gaatgagtct aaacatacca gagctaagag aaagagtcaa atcaatagca
     2521 gaaactcaga gttcaaaagt aaagacttta ggagtaaagg aaattacagg agaaatcatt
     2581 gatttatctg tagggcgatt aggtatatct atgttagaag ttacacagga catgatttac
     2641 tctaagacta atactttaaa agatacgtat attttttctc tagcagattc attggcaatg
     2701 gctataatag atgcacctaa ggttattatg agagttgcaa atgtaaaaag ttttaagttg
     2761 atagaacaac aagataggct tatagcaaaa gcagaagtgt atagaaatat agataaaaag
     2821 cattatgtaa aagttgttat caacaataaa gcccaagagc aaatatttag aggaaagttt
     2881 atctttgaag aattagatta ggagggaaat tatgaaaata gtaattgatg gaatgggtgg
     2941 agataatgca cctaaatcta atgtagaagg cgcagtaaat gctataaaag aatatcaggt
     3001 tgatttaata atcacaggag ataaagattt acttgaaaag gaattttcaa attatgaatt
     3061 tgatagaaat aaattagaga tagttcatac tactgagatt atagaaaacg aagataaacc
     3121 agtaaaggct ataagaagta aaaaagattc atctatggta gtagcattaa atcttgtaaa
     3181 agaaggaaag gcagatgcta taatatcagc tggaaataca ggagccttac ttgctggtgg
     3241 attatttgtt gttggtagaa taaaaggaat cgatagacct tgcttatgtt cagcaatacc
     3301 aaatgtaaag cgaggaatga cacttatagc agactgtggc gcaaatgcag attgtaaacc
     3361 taagaatcta gttgaatttg cagctatgag taatatttat tcaagaaaag ttttaggtct
     3421 tgaaaatcct aaggtagcat tagcaaatgt tggactagaa gaaggcaaag gcaatgattt
     3481 agtcaaaagg tcatatgaag aaattaaaaa attagattta aattttatag ggaatgtaga
     3541 agctagagaa gttataaatg catacacaga tataatcata tgtgatggat ttacaggtaa
     3601 tatattactt aaatctgctg aaggtgtagc actttcagtt atgagtctta taaaggaaac
     3661 ttttatggcc agtacaaaaa gtaaaatagg agcattactt ataaaggatg atttaagaaa
     3721 attaaaaagt tttatagatt attctgagta tgggggagct ccacttttag gtctaaatgg
     3781 aggagttata aaagctcatg gtagttctga tgctaaagct ataaaaaatg ctataaatca
     3841 aggaataaaa ttttcaaagg gaaaagtcgt tgaagatatt aaccaattta taagcaaata
     3901 caatgaagaa aataaaaata acgaagatga ataaactcgt tttggaggtt taatatataa
     3961 tgaatacaaa ggctggaata ttaggagtag gaagttatct acctgagcaa tcatatgata
     4021 attttcactt cgaaaaaata atggatacat cagatgagtg gataagtact agaactggta
     4081 ttaaggaaag aagatttgcc aaagagtctg aagcaacttc tgacttagca tctaaagctg
     4141 ccttaaaagc tatagaatgt gcaaagttaa acgtagagga tatagagctg attatattag
     4201 ctacaattac tcctgatatg tctttgcctt caactgcatg tatagtacaa gatgcaatag
     4261 gagcagtaaa tgctactgca tttgatatat cagcagcttg ttctggattt gtatatggag
     4321 taactattgc aaagcaattt gtggaaacag gttgctataa aaatgtgctt gtaataggag
     4381 cagaaacttg ttctaagttt ttaaattatg atgatagaac aacagcagta ttgtttggag
     4441 atggagctgg agcagctgtt attggccctg taaatgaggg tggaatactg tctacacata
     4501 tgggctctga tggtaaagga aaagattgtt taaaagtacc tgctggaggt tcaagactta
     4561 aggcaagtaa agagactgta gaagctaatc tacatacaat agaaatggca ggtagtgatg
     4621 tgtttaaatt tgcagttaga aagatggcag aaacttcttt aagagctctt gaaaaagcaa
     4681 atctaaatac aactgatata gactacttag tgccacatca agctaatata agaatcatac
     4741 aagcttcttc taagagatta gagcttgata tgaaaaaagt ttatgtaaat atagacaaat
     4801 atggaaatat gtctgctgca tctataccag tagcattaga tgaagcttat agagaaggta
     4861 aaattaaaaa gggtgataat gtagttttag ttggctttgg tggaggtcta acttgggggg
     4921 cttcagttgt caagtggact ttatagtata aaatatctaa aatgatttaa ttaacaaaat
     4981 taaacttata cacaaaataa agttgattaa taataaaaag tttactttta tgatttcaag
     5041 tgaaggagaa ttgtatgaat aaaatttgca aaatattaaa tataaaatac ccagttatcc
     5101 aagggggaat ggcatgggta gctactgcat cattagcaag tgctgtatct aatgcaggag
     5161 gacttggcat aatagcagca ggaaatgcac caaaagaagc tataaagaaa gaaattgttg
     5221 agtgtaaaaa attaacagat aagccttttg gagtaaatgt aatgcttatg tcgccatttg
     5281 ttgatgatat aattgatttg attatagaag aaaaagttca agttattact actggtgctg
     5341 gaaatcctgc aaagtatatg gatagattaa aggaagctgg aacaaaggtt attcctgtag
     5401 tacctacaat agctttggca caaagaatgg aaaagctagg agctacagca gtaatagcag
     5461 aaggtactga aggtggagga catataggag aacttactac tatggtctta gttccacaag
     5521 ttgctgatgc tgtaaacata cctgtaatag ctgctggagg aattgtagat ggtagaggaa
     5581 ttgcagcatc atttgcatta ggtgccagtg cagttcaagt aggaactaga tttatttgca
     5641 gtgaagagtg ttctgtccat tcaaactata aaaacttagt actaaaagca aaagatagag
     5701 atgcaattgt aacaggaaga agtactggtc atccagtaag aacattaaaa aataaactat
     5761 caaaagaatt tttaaagatg gaacaaaatg gagctactcc tgaagaactg gataaaaaag
     5821 gtacaggagc tttaagattt gcaacagtag atggagacat agaaaaaggt tcatttatgg
     5881 caggtcaaag tgctgctatg gtaaaagaaa taacaccttg taaggaaatt atagaggcta
     5941 tggtaaatca agcaagagag attatgccag caatagaact gtaaatcagg aggagcaaga
     6001 tgggaaaagt tgcactagta ttccctggtc aaggagctca atatgtaggt atggccaagg
     6061 atttatatga aaataatgct gttgctaaaa gtgtaataga tgaggctagt gatgccttaa
     6121 atatggattt aaaaaacctt atgtttaatg gaaatgaaga agaactttct aaaactgaaa
     6181 acactcaacc tgctatggtt acacatagtg tagcagtatt aaaagcggta caagctcaaa
     6241 tagatttaaa atataatgca tgcttaggat tatccttagg agagtatagt gcgttggttg
     6301 cagcagatgc aattgacttt aaagatgctg tatgtttagt taaaaaaaga ggtaagttta
     6361 tgcaggaaac agttccagca ggtgttggag ctatggcagc agttttaggt ttagatagaa
     6421 atgtacttga aaatgtggtt aaagatatac aaggaggtat tgtagaagta gcaaattaca
     6481 attctcctgg acaaatagtt atatctggtg aaaatgaaaa gatagaagaa gcaatggtaa
     6541 aatgcaaaga agcaggagca aaaagggcag ttaaattaaa tgtaagtgga ccttttcact
     6601 catcaatgtt aaaagaagct ggtgtaaaat tggccagtga attagaaaaa gttaacataa
     6661 ctaaacctaa agttgatgtg gtggcaaatg taaatgcaga ttactataaa aacgaaggta
     6721 aagacctgct tataaaacaa gtaagttcat ctgttctttg ggaagattct atagaaagac
     6781 ttttaaatga tggatatgat acttttatag aaatgggacc tggaaaaact ttaaaagcat
     6841 ttattaagaa aatagcttcc aataaaaaag caaatgtcaa tatttataat atagatggta
     6901 tagattctct taatgaattt gtacaaaatt atagaaatgg agagatataa tgataaatct
     6961 tacaggacaa gtagcagttg ttactggtgg ttctaggggt ataggaaaag aaatagcaaa
     7021 aaaactagca tcttttggag ctgatgtagt aatcaattat acttctaaag aagatgaagc
     7081 actaaaaact aaaaatgaaa tagaaagtat gggggtaaag tgtacctcta taaaatgtga
     7141 tgtgtctaaa tttgatgaag tgaatcaaat gatagattct gttgtaagcg aatttggaaa
     7201 aattgatata ttggttaata atgcaggcat aactaaagat ggtctgctta tgagaatgaa
     7261 agaagaagat tttgatagag ttatagatat aaacttaaaa ggtgtcttta attgtacaaa
     7321 agcagttact aaacctatga tgaaaaagaa gtatggaaga ataataaata tgacttcagt
     7381 agttggaatt atgggtaatg cagggcaaac taattattgt gcatcaaaag caggtgtaat
     7441 tggatttaca aaagcttctg caagagagtt agcatcaaga aacataaata taaatgcagt
     7501 agcacctgga tttatagaaa cagatatgac taaagtacta agtgatgatg taaaagaatc
     7561 aacactagca aacataccaa agaaatctta tggtaaacca gaagatgtag ccaatgccgt
     7621 agcattttta gttagtgaca tgtcaagtta tataacagga caagtaataa atgtagatgg
     7681 tggaatggta atgcaataat aatttaaaaa gtagagaaat ctattaaaat aaaaaatttt
     7741 aggaggtaca caatgtttaa taaagttgta gaaataataa tagagcaatt aggagtagaa
     7801 gataaagaaa taactatgga aacatcatta atgaaggatt tagaagcaga ttcattagat
     7861 gcagtagaaa taataatggc acttgaagat gaatttggta tagaaatacc agatacagaa
     7921 gcagaaaact tcaaatctat aggagatata gttaagtata tagaagctaa taaatagtcg
     7981 agctttaaaa aagtggaggt atccgatgaa taaaagagta gtaataacag gacttggatg
     8041 tgttacgccg ttgggaacag gaaaagaaga gttctggagt aatataaaat ctggagtatc
     8101 tggtatagat aaaataacta attttgatgc tagcacatat caaactcaga tagcaggaga
     8161 agttaagaat tttcatccag aagaatatat aagtaaaaag gaattgaaga gattagataa
     8221 atttgctcaa tttgcaatag tatctgctaa attagcagtt gaagatgcaa atcttgattt
     8281 agataaagtt gatagagaac gttttggtgt tataataggt tctggaatag gtggagttga
     8341 agctatagaa actcaacata aaattctttt ggaaaaagga aataaaagag taagttcact
     8401 ttttgtacct atgatgatag gaaatatggc agctggtcaa gtttcaatat ttttaggagc
     8461 aaaaggacct aacactaatg tatgtacagc ttgtgcatca ggaactcatt caataggtga
     8521 tgcttttaag gtaattcaaa gaggtgatgc agaccttatg gtagcagggg gttctgaagc
     8581 tgctgtaaca ggtcttgctt ttgctggatt ctgtaacatg aaggctatgt caactagaaa
     8641 tgatgaccct aaaactgcat caagaccatt tgataaagac agagatggtt ttgtaatggg
     8701 agaaggagca ggtattgtta tcttagaaga cttagaacat gctttagcaa gaggtgctaa
     8761 aatatatgca gaagtagttg gatatggttt aactgctgat gcataccata tgacaactcc
     8821 agctgaaaat ggagagggag cagctaggtc tatgaatatg gctttgaaag atggaaatgt
     8881 tccattagaa gaagtagatt atataaatgc tcatggaact tcaacttatt acaatgattt
     8941 atatgaaact atggctataa aaactgtatt tggagaaaag gcatatgact tatgtgtgtc
     9001 atcaacaaaa tctatgactg gtcacttatt aggagcatca ggtgctatag aggctgttgt
     9061 ttgtgctatg agtatagaag atagttttgt gcctccaact ataaacatac aagaagttgg
     9121 tgaagatttg gatttagatt atgttccaaa tcaaggtaaa gaaaaaaata ttagatatgc
     9181 actatcaaat tctttaggat ttggtgggca taatgctaca atagtactaa aaaaatatgt
     9241 ataaataaat attaaatata aaactaaaaa acaagcactt gttttaactg tataaattta
     9301 tattagctta aatttaaagt ctcctaaatt attttaggag atttttttat taagatttaa
     9361 gtctaggata tgttgtaaat cattttgata tattgtatta ttaattatta agaggttatt
     9421 tatatatgca agttttcatt ctacaatata caaaaggagg actaaaattt tgaattttag
     9481 actaccaaag aaaaggttac ccaaaaagat aatttttata tgtataatac ttattagtat
     9541 tattttgttt ataaagtatg accaaaatgg attgaaagaa agagaaatta taaataaggg
     9601 aaaatatatg tctgaaagcc ttgtggcaga taaggagtat gatgaagcaa aaaaagtggt
     9661 taagacttct tttgaatcta ttccagaaaa aaaatacaat aaagtatata atgaagtttg
     9721 ggatatgtta aagactattt catatgttcc agatggaacg aagatagtca ttgattcttt
     9781 agaaaagatg agagaatcag atgtgaaatt atctgatgaa tgtagattta atatagaaaa
     9841 aagattagca tcagtgtata taatggataa taattattct aaggcagtag atttaactgt
     9901 aaagtctata aagttggctg agaaattggg gaataattat gaaaaagcaa gactagatgt
     9961 tgatttggcg agcatatttt taaagatagg tggctatgaa acaggagaga aattaataaa
    10021 ggattctttt aaaatagata ttgatgaagg tgaaaaaaat ggaatggtta gattatatgc
    10081 tattataaat ctagcagaaa tatatgtaga agagggcgaa tatgataaag ctattgaaat
    10141 aagtagcagg ataaaagatt ataaaaagtt tgtaaatacg gataattaca atgattttga
    10201 tataatggcc tctataatgc agtcaaatgc gtatgtgggc aaaaataata tacaaaaggc
    10261 aaagactttt ttagaaaaag ctgattatct tataaaatca gatagaacag tttatatatt
    10321 agataaagat atgtactatt atatggcaat gggaaactta gaatataaaa gcgaaaatta
    10381 taattctgca atagataatt ataaaaaaag tttagagata tctactaaaa gaaagttaac
    10441 ccaggagaaa ataaaaaatt taaataagat attaaatata tatgaaaaac aaggaaatat
    10501 agaatcttta aatgagtatc aaaagatgtt aataaatgaa tataaagaga acaaaagtat
    10561 aagagattca gagactagtt tctatataat tgataaagta tcaaacgaaa gtaagctatt
    10621 tgaaaaaact aaaaaagaaa taaaatatta taaaatatta tttacagtaa ttttaattgc
    10681 tatattaggg tctacttttt tatacaaccg attgaagtat tttaaagcac aaaatttaca
    10741 tgatggtctt acaaatgttt ataatagaaa aagttttgat attatgtatg aaaagtacag
    10801 agagaaagat gataattttg cacttgtgat gatagatatt gataatttca agctcataaa
    10861 tgataattat ggacataaat ttggtgacac tgttataaag ggaataacaa ataccatttg
    10921 tgttatgtta gaacaagatg ataaagtctt taggtacgga ggagaagaat tttctgtatt
    10981 aataagaaat aaatctggtg atgaagtagc agatataatt gacagtataa gggttgcaat
    11041 tgcaaataaa aagtggaatg atgatgtgac tgtgactata agtgcaggtg tagctcatgt
    11101 ttcagatagc aaagatgtac tagaagaagc tgataaaaat ttatataaag caaaacaact
    11161 tggtagaaat aaagtagtat ataagtaatt atatactata taaataagag gtataaatat
    11221 gaaacaatca atagctatag taactgataa aataactaaa ctatcatctt tcttagaaga
    11281 aaatatacgt ttggttttag gtaattatat tgatataaac cactattatc tggaagtttt
    11341 aaaagagaat gataaaatac aaggtgattt tgtactagta atgaatgatg atagattaaa
    11401 cagtatgaga aagcatttag tacaagatag taagattata gtgataagga gaacacttaa
    11461 agaaaatgaa atatatggat tattttcaat accaaatgga actgaagtat tggttgtaaa
    11521 tgatacaaaa gaaactacct tagaaacgat aagcttattt tacaaaatag gagtaaaaaa
    11581 tttaaagttt actccatata ttgaaggaaa aatttataaa aatatagaca tagctataac
    11641 acctggagta aagggaaaag taccaagtta tataaataaa gtaatcgacc ttggaaatag
    11701 atatattgat atatctacct ttatagaaat aataaatggt cttagaatag atgttaaaga
    11761 aatacgtaaa aatttaatgg catattctga gagtttaata agtttagata atggagttaa
    11821 agataattat aggcaactgt ttttaaaaat agaagaacaa gatgttatat tgaatctatc
    11881 aaaagatgga ataatattta cttcagcaga tggtataata aacacattta ataaagaagc
    11941 tttaaaaatt ttaggtataa atgaaaatcc acaaggcaaa aacatagaag atatcatttc
    12001 tgaagattta aatattttac ttgaagacaa agaaataatt gatgaagtgg tatttacaaa
    12061 taaaaaatat ataaatgtaa ataaaaaaag tattttttct atggggaata aagctgggat
    12121 atattataac ttacaagaaa taacgtatat aaaaaaatta gaacaaaatc ttactaataa
    12181 attaagagaa caaggtcaga tagcaagata tacatttaaa gatataaaaa ctaaaaataa
    12241 aaatatggta aagtgtgtag aattggctaa aaaaatatct aaatcagacc tttctgtatt
    12301 aataatagga gaaagtggta ctggtaaaga attattatca caatcaattc ataatgcttc
    12361 caatagaagt aatcaaccgt ttatagcagt taattgtgca gcagttccag atagtctatt
    12421 agaaagtcaa ctgtttggat atgaaaaagg ctcttttaca ggagctttaa gagaaggaaa
    12481 aaagggtcta tttgaacttg ctaataatgg aactatattt ctggatgaaa ttggagatat
    12541 gcctccattg ctacagacaa agttactaag ggtattacaa gaaaagcagg ttatgcctat
    12601 aggttcccat aatgttataa acatagatgt tagggtcata gcagcaacaa acaaaaactt
    12661 actaaagatg attagagaag gaaaatttag agaggacctg tattatagac taaatgtatt
    12721 gccaatagat gtaccatcat tgaggaatag aaaagaagat ataataacac ttatgaattt
    12781 ctttttgaaa aacaatataa gactttcgcc agaagttata actattttag aaaaatataa
    12841 ttggccagga aatataagag agttacaaaa tgttgcttct tatgtaagtc taatgtgtga
    12901 tagtatggtt tttagagatg acttaccacc atatataagg gtagaatgtg ctaacaatta
    12961 tgaagatgga tttggttata caagaaaaga atttgaggaa atactgagga ttctatatga
    13021 aagaagagag tcatcctctg gaattggaag agggtttata atcgaaaagc ttatacagga
    13081 aggtattgag atttctgaaa gcaaagttag aaaggtgcta tcttatttaa gtgaatgtgg
    13141 atacatatca tctaaatctg gtagaggagg aagtcaaata acctcagagg ggataaaact
    13201 ttataataat ataaattaag ctaagtattt gatattgaaa tcacatattt tgaatttaga
    13261 tatttaaata ataataaact tttatattta tagagattaa tttatattat gtcagaaaga
    13321 atattgtaat ttataattaa atattaatag gtttttaaaa taggattaga taggtaatag
    13381 gttaagttag aataacccat tacctatttt tatttgtaag aaaagtgtac atagaattag
    13441 aagtaaaaaa tacaactaat aaaactattg aaaatactag ataaataata aataaaaata
    13501 attatagatt ttggcataca tattgctaat tatagagata agacgacaaa ataattggga
    13561 ggtttaatga tgaaatacaa aacaacaggg acttgtgcta cagaaattga atttgaagta
    13621 aaagaaaata aagttacaaa tgtaaacttt ataggtggat gtgatggaaa tttaaaaggg
    13681 ctaaaagtat tagttgaagg catgaatata gaagatgtaa tacaaaagtt aaaaggaata
    13741 gagtgtaaaa caaagccaac atcttgccca gaccaactgt ctctagcatt ggaaaattat
    13801 atgaatataa agtaatacat aaaacaatcg attagttagg agtttaatta tgaaaccaat
    13861 aatagggatt ttaggaaatt taataataat ggaaaatggt atgtttccag gtcttgaaag
    13921 gtcatatgta aataatgatt atataaatgc agtgctaaaa ggtggcggta gtccagtaat
    13981 aattcctgta aatacagata aagaagttat aaaaaaacag attgaaatgg ttgatggagt
    14041 cttaatatct gggggatggg atatcaatcc tcaactgtat ggagaagaaa caagagaaga
    14101 aacgacattt atatatccag aagtggatga atttgattta attgcaataa gtatagcatt
    14161 agaacttaaa aagcctatat taggtgtatg taggggacta caaattttaa atgtaagttt
    14221 aggaggaact ttatatcaag ataataatct tatagaagga agttatataa aacatacaca
    14281 atcttctaaa agacatgtag ctactcataa agttgatgtt aaagaaggaa gtattttaga
    14341 gggaatttta ggaaaacaat tacttacaaa tagttatcat catcaaagtg tcaatcagct
    14401 tggaaaagga ttaaaagcca tagcatactc aaaagatggt ataatagaag ctattgagaa
    14461 agaggatgaa aattttgtag taggagtaca gtggcatcca gaaatgatgg tagattactg
    14521 tgataaaatg gaaaaactat ttaaatattt tataaatatt tgttcgaatt tataatatac
    14581 cttgggggga ttgaacatga acgaaaagaa caaaatggga ttaataagta ttatactttt
    14641 aggtattaat gcagtagtag gagctggagt ttttctatta ccaggagatg caatgaaatc
    14701 atttggtgta gcaagtatat ttgtatatat atttgatatg ttgttagttt tatctatggc
    14761 attctgtttt gctgaggttg caggtaaatt caataaaaat ggtgctgcct atgtatatac
    14821 aaaagaagct tttggagatt tttgtggttt tgaagtaggt cttatgaaat gggtaatagg
    14881 atgtatttct tggggagctt taatagtagg ttttcctaca tcattatcag cagtatgggc
    14941 tccagcaggt gaaccacata tacaaaaaat tataattgta gctatgatag ttgggcttac
    15001 aattataaac ttattaggtg tatctctatc aaaaattgta caaaatgtta taactgtagg
    15061 taaactaatt ccattaatac tatttatagg aataggtata ttttttataa aaggagttaa
    15121 ttttacttct tcaacaatgg tacctccagg agctggagca acagagtttg gagcagcagc
    15181 acttttaatg ttttattcat ttacaggatt tgaatctata gcagttgcag cagaagatat
    15241 ggaaaatcca caaaaaaata taccaatagc aattataagt gtaatagtaa ttgcatcaat
    15301 aatatatata ttaaatcaag ttgtatgtgt tggtatacta ggagattcgt tatctagtac
    15361 atctacacca gtggcagatg ctgcaagaat atgctttgga aatatgggag ctggattggt
    15421 tacctttgga acattggttt ctgttggtgg aatttgtatg tgtggtgctt ttgtaaatcc
    15481 aagaagttgt gtagctttag ctgatgataa aatgttaccg agaatatttg caagaaaaga
    15541 taaaaaaggt acaccatatg tagcgataat agctactatg ttaatcacta taccaatagc
    15601 tctatctgga agttttgctg aattagcagc aataagtgca gttgctagat tcatacaata
    15661 cataccaaca tctttatccg tattagtgtt tagaaagaaa agaccagaat tagtaggtac
    15721 ttttaaaact ccttttggag cagtgattcc tctaattgct gtatgtgttg gaacatggtt
    15781 gttattccaa gcatctatgc atcaattaat tatgggatta ggagctttgg caattggagt
    15841 accattatac tttataatga agtcttataa tagaaaaatc tatggtgaaa atttaaagaa
    15901 aatagtttaa ttatgttata acaatttatt gagttaaaat tagacattgg cagaaaatta
    15961 aatacaaata agtatctaca aggctatatt aaaatattat taatttttta ttttaatata
    16021 gccttatatt gttatcaata aatgaaaaat attgtaacaa cagaagaaca atagtattat
    16081 gtttatagac aaaaaactta ttaggaggta aatttatgcc aacaataaca ttaaaaaatg
    16141 gtgtagacgt acttattaga gaaggtgtaa gagaagatgc tcaaagcatt atagattttt
    16201 ataatgaagt gggtggagaa actcattttc tgtcatttgg aaaggatgaa tataaaatat
    16261 ctttggagga acaagaaaat gctatagaat cagcaaaagc atctgataat tctgttaaac
    16321 tgattgcatt tatagatggt gaaatagttg ggattgcaac gattgattct aatcaaaaag
    16381 caaaaggaaa gcatgttgga gtattaggaa ttgttgtaaa agaaaaatat tggggaattg
    16441 gattgggtaa aaggcttatg ttagacctta tagaatggtg taaatcaaat ggaataacca
    16501 agaagataac tcttgtcact aatgaagaaa attataatgc tataggatta tacaaaaaag
    16561 ttggatttga agtagaaagc atactaaaga aagaatgtta ttataatggt gtttatactg
    16621 atttaatagg aatgtcactt ttattgggaa tataattagt gtacatttta tctcgaaaaa
    16681 agtatattaa gtatggtata attaaaaaag aatataactt aatataattt tggaggacga
    16741 attatgaaaa atctgtgtaa aatatcagat gattatttaa acagcaagct taagtattta
    16801 aagttattat caaaacaata tccaagtata tcaaaagcaa gtacagaaat aataaattta
    16861 gaggctatat taaatcttcc aaagggaaca gagcatttta taacagatgt acatggtgaa
    16921 tatgaaccat ttgtacatgt attaaaaaat ggttctggtg taataaagag aaaaatagaa
    16981 gaattgtttt caaatacaat aagagacagc gaaaaaaaaa tgttagcaac tcttgtatat
    17041 tatcctgaac aaaagttaga cctaataatt aagcaagagg aaaatataga tgatttttat
    17101 agaataaata tttatagact tatagaactt tgtaaatatg cttctagcaa atatactagg
    17161 tctaaagtta gaaaactgtt accagaaaat tttaaataca taatagaaga acttttgcat
    17221 gagcatgtta aaagtgaaca taaagaagag tattataaaa gtatagttga aacaatagtt
    17281 gacataggaa tagctaagga gtttataatt gctatttcta cagttataca aaaattggtt
    17341 gtagatagac ttcatgtaat aggagatatt tatgatagag gtccaagacc agatataatt
    17401 gtcgataagc ttattgaaca tcattgtgta gatattcaat ggggaaatca tgatatattg
    17461 tggatgggtg cagcctcagg tgaaaaaact tgtatagcaa atgcactgag gatatcagct
    17521 agatatgcga atttagacat tgtagaagac atatatggaa taaatctatt gcctttagcc
    17581 acttttgcta tagaaatgta taaagatgac ccttgtaaag aatttattcc aaaggttaat
    17641 gaccaaagtg taacaacaac agaaaagtct ttaatggcaa aaatgcacaa agcaataagt
    17701 ataattcaat ttaagcttga aggtgaggtt atcagacgaa gacctgaatt tgaaatggaa
    17761 catagactct tgcttaatat gataaactat gatgaaggta cgattaattt aaaaggtaaa
    17821 acatataaat taaaagatac ttatttacct acaatagaca aaaaagaccc atataaatta
    17881 actatggaag aaagaaatgt tattgataaa ttagtatctt catttagagg tagtgaaaaa
    17941 ctacaaaaac atgtttcatt tttgttttct aaaggaagta tatatttaaa ggctaactca
    18001 aatttactga ttcatggatg tgtaccgtta aatgaagatg gaagctttat gtctatgaat
    18061 ataatgggaa aagagtataa aggtaaggct cttatggata agatggagtc tctagcaaga
    18121 gaaggatttt tctttaaaga taaggcagaa gaaaaactgt atggcatgga tattatgtgg
    18181 tatttatgga ctgggaaatg ttcatcatta tttggtaaag atgatatgac tacatttgaa
    18241 agatacttta tagctgaaaa agagactcat aaagaaaata agaacccata ttttaaactt
    18301 agagaaaatg agatggcttg taaaagatta tttgaagaat ttgatttaga acttgatgaa
    18361 tctcatataa taaatggtca tgttcctgtg gagagtaaaa atggagagag cccaataaaa
    18421 gctaatggta aaattctcgt tatagatggt ggtttttcta gggcttacca aaaaactact
    18481 ggtatagctg gatacacttt gatatacaat tctagaactt tgcaattagt atcgcatgaa
    18541 ccatttaatt ctgctgaaga agccattgca aatgaaagtg atatcttatc gacaactgtt
    18601 gtagtagagc ataaggcaaa gagaaaaatg gtgagagata ctgatgaagg aataaagata
    18661 caagaggaaa tagaagattt aaaactactt ttaatggctt ataagaaagg gctaataaaa
    18721 gaaatgtaga tgttatagag gcacttcatg attgaagtgt ctttttgtta atacttatta
    18781 aatatattat aaagattaat aatagtagtt tatattatac ttactaagtg aatccaagat
    18841 aaatgggttc acttttttta tttataaaca ttattaaaaa aatatagatg ttttggttat
    18901 attccttgtc ctttataaat agaattatat aaacaaagga ggaggacaag agatgaataa
    18961 actttctgat gaaataataa actctctttc tacaactata agagaaaagg tagaaaaagt
    19021 ctcaaacaat aatctgaaca tagaagaaat taggttacgc tcacaaaaac cattgatact
    19081 aaatgcaaac tctaaagatt atttttacaa tcaaaaaact atgacacttg atttaaatca
    19141 acaaaactca tatgtagtaa caagagaaga tgtagagcaa acttttcaaa taatatgtaa
    19201 atattcaata cattcattta tggatgacat aaaaaaagga ttcataactt tgagaggagg
    19261 tcatagagta gggttagttg gaaaagcaat agtagaagat ggacaagtca aaaatataaa
    19321 gcatatatca tctttaaata taagggtatc aagggaaatt attggatgct cggataagat
    19381 tttaagtcat atcataaagg gaaaaaatca gattaataat actttgataa tatcaccacc
    19441 tcaatgtgga aaaacaactt taataagaga tatagtaaga aatttaagta atggtaatga
    19501 agattatgga tttaaaggtt taaaagtggc tttagtagat gagcgtaatg agatagcagg
    19561 agcttatcta ggtgttccac aaatggatgt gggaataagg actgatataa tagaaacttg
    19621 cccaaaagac ttgggtatta caatgctttt aaggtctatg tcaccaaatg ttattgtgac
    19681 agatgaaatt ggaagtgaaa aagaaataaa ggcattatac acagcattaa atggaggtat
    19741 aggattaata actactgtgc atggagattc aatagaggat atacaaaata gaaaagaatt
    19801 aaatagactt ctggataaag agttatttaa aaaggttata attctttctg caaaaagagg
    19861 agcaggaaca attgaaaaaa tttatgattt agaagaaaag agatggtatt ttgcaaatta
    19921 aaataattat tatagctttc ttgataggaa gtagttattt gataggtgaa caaatataca
    19981 aaacttatac tcgtagacat aaacaattaa acgatttaat aagagtttta gaaattttga
    20041 ggatggattt atcttttgga ctttatacat tagaagaaat ctttaatagg ataggtggaa
    20101 ataaagagtt ttgtttttgg aaatttttct atcaaatatc agaaggactt cacaatgaac
    20161 aaagtaaaac tttggaaata ataatatcag aaaatattga tgtcttaagt aaagaaactt
    20221 atctaggcaa taaagaaatt gaggaactga aaaatctaat acttacttta gggaaaagcg
    20281 atatagagtc acaacaaaga atgatagatt tatctataga aaatttgaaa aaacaaacct
    20341 atgagacaaa ggaagatata aacaaaaaag gcgtattgta taaaaaatta gtgacattca
    20401 taggtatagg tatatgtatt atattgattt aatatgggga ggtttattat ggaaatatca
    20461 ttgatattaa aggttgcagg tgtcggtatt ctaatatcag tattgaatat gatattggaa
    20521 aaaacagata gaaaagattg ggcaggtctt actacattag caggtgtaat aatcgtattg
    20581 ggtatggtaa taacagaaat aagtgacttg tttaatactg taagaacaat gtttcaactt
    20641 tactaaagag gagggagtaa tttggaaata atgcaattaa taggaattgc aatcatttca
    20701 actacactct gtttagtaat taaaaaagat agaccagaga tagcaaattt catagctata
    20761 ataactggag taataatact attgtcagtt atgtttaagc taaattttat agtagatagt
    20821 atacaagatt tggctaacaa ggctaacatc ccaactatgt atatatcatt aataataaaa
    20881 ttaattggga tagcatactt aatggaattt gcgatacaac tttgtaaaga ttgtggggaa
    20941 ggaaacattg cttcaaaact agaatttgga ggtaaaataa tagttatgtc aatgtctttc
    21001 cctatacttt tgtccattgt agaaatggtg gtgaatataa ttccatagat gtgtatcttg
    21061 gaatgaatat aagtatatta gagagctttc gttgtattga ggtttataga gtgaagagag
    21121 gcatggttgc ttatgaaaaa aagattttta ccaatgatta taggtttttt atttactttc
    21181 ttttttgtaa atatctttgc tattatgata tttgctaatg aagtaccaag tagtgaagat
    21241 aaagaatatg gtgaaacaaa aaatagcata gataagtata tagatggtca attagacaag
    21301 ctagatataa atgagattca agactatata aataaagaaa tagttattaa tgatgttaat
    21361 ttaaaatcat ttgtaaaaga tttaattagt ggagaaaaaa atatcttaga tttattcaat
    21421 aaagatggat tgaagatatt gatgtttgat gaatttaaag caagtttaaa ggtagtagca
    21481 gtaattttgg tactggctct gttatcatct attttaaaga gtctggaaaa ctctttttca
    21541 tcaggtgcag taagtcaaat agcaacatat ataatattta tcacaatggt atcacttaca
    21601 cttattggtt ttaaagatgt attacagatt tgctatgatg caatagacca tactgtaggt
    21661 cttatgcaag ttataatgcc aatattaata acatttttac ttttaattgg attcccaata
    21721 acgtcgacta ctttgaatcc tatcttcata ggaggagtaa catttataaa tgtatttttt
    21781 aaaaattttc tatttgtatc tattactgtt gcatttggaa ttttaataat aaacaattta
    21841 tcaaaaaata taaggctaaa gagatttttc tcctttgtaa aacaaataaa ttatgtttct
    21901 attggagcta tgtttactgt ttacttaggt cttgtatcca tacaaggatt gtatgtaacc
    21961 agttttgata aattcagtgt aaagactgct aaatttgcta taggtaattt tattcctgtt
    22021 gtaggtggat ttgtatctga ctctgtggat attttattat cttcatctca gcttataaaa
    22081 aatatatttg gaggaatagg acttatctta cttgttggaa tttgcttact tccagtaata
    22141 aaaattttat cagtaatagt agtgtacaaa ttagcagcaa taattgtaga gcctgttgga
    22201 gaggatggaa tatctaattt tttaaatgaa gtggcaaatt taatgataat aatgctagca
    22261 tcagtaattg ctataacagt aatgttcttt gtaactgtag ctatcttgac ctcgataagt
    22321 gtcgtaagtc aagggtaatt tatatatcag tgaaaaataa ttacatttaa tctaatgttt
    22381 ttagagagga ggatgctatg ttagagggca taaaggcatg gatagttagt gttttgatag
    22441 gagcatttat agtaaatata gtagatatga ttttaccaag ttctaagata aaaccttatg
    22501 tcaacctagt tttaaacttt atgtttgtat ttattgtaat aactccagta gtaggatttt
    22561 tttcaaaaga tatgagttta gaagatagaa tattaaaatc tatgggaaac tacaacaagc
    22621 agtatgtaga tagtacaaat gctttggcaa aagagactgg aaataatagt ctatccaagg
    22681 gatatgagga tgggttaaaa gaagtactta aattaaaact tgatgaatat ggttatgatt
    22741 tagaagatat agagcttaat ggggcaaata taaataatat aaaaataaaa gaaaagaata
    22801 atagtgcaaa aagtaataac agttccaatg aagaaaataa aaataatagt actaaaagag
    22861 aggaagaaaa cataaactct aatgacaaag aaaattctaa acaagtcttc aaaaaaggta
    22921 cagagtatgg gttgaaccta aatgaagaaa aattaaaaaa cgacttaata aaggtgctgg
    22981 atgtttctat agaagatata caaattgata aataggagtt gggagaatgt tgaaaaactt
    23041 aaatgacaaa gataagagaa aggtatattc actaataact atagcaggaa tatgtgtggt
    23101 ttcattagta ttattatctt gttttcctag tagcaaaagc gataaacaag ttggaaaaac
    23161 agagactaat aaaaatgctg aaaagcaagt cactaaagaa caagaaaaag atgatttaga
    23221 gtcaaaatta acagcaatac tatctaaaat tgatggtgca ggagacgttg atgtaatggt
    23281 tacatttgaa tcaagtgaag aaatacaacc tgcttttaat tcaaataata caacagaaac
    23341 aacagaggaa aaagatgcac aaggtggaga aagaacagtt acgacttcta gtgaaaataa
    23401 aacaatgata acttcaaatt caagtgaccc tgtagttata aaaactacag agccaaagat
    23461 aaagggagtg atagtggtag caagtggagc aagtgaccca agtgttaaag agacacttta
    23521 tagtgcagta caaacatcat tacaagtagc tggtcatcaa gtagaaatat attctaaata
    23581 aatacatata accgggggga taaatgaatg aagtttaatt ataagggaag aggatttgta
    23641 ataataactt taactgcaat gttagtggta gtaggaacag tgaattatca actaagtaag
    23701 aaatctttat tagaaacatc aaaagagttt aaggcatatg aagaagcaca gttgcaaaaa
    23761 aatactgatg agagtgatag tagtaagaca gaggattcta gtaatgtaga taaacaagat
    23821 ggtaaggaaa gtgctgatat tgatatagtt gatagcaaag ctagtaaagt aaaagaaaag
    23881 gcaactgaga caagtaagga aataaaagct caattatcat cagagaaaaa tatgaaaaag
    23941 gcatcttata ttttagatat gaaaatgaat agagaaaaac aaagaaatga gttagttcaa
    24001 gatttaaatg aaatgataaa taatccatct acaacagaag aatctagaaa agaagcttct
    24061 aatatgaaat taaatatagt taaaattcaa gaaaaagaac ttcaaataga aaaccttttg
    24121 agtacaaaag ggtatgaaga agcgttagtt tatataagcg ataacaaagt taatgtagtt
    24181 gtaaatgaag ctaaattaga aaagaaagat gcagcaaaaa tatttgattt agttgcagaa
    24241 caagccaatg tgaagtatga aaatattaaa cttacaaata ataatagtaa taagtaaagt
    24301 ataaacctca gaattaactg aggttatttt cttgcaaagt agacttgtat ttggtattat
    24361 tatacttata aaaatagttc cttaaaatca ggaggtaagt tttaatatgg aagataataa
    24421 atttggacaa gtaaaaatat ctaatgatgt aatagctaca atagctggtt tagcagcatt
    24481 agaagttgaa ggcatagaaa ctacagcaac attaacagat aagttgttaa aaaataatgg
    24541 agtaaaaata caaatcgaag aagaggatgt taatttagat gtaatggtta cgataaagta
    24601 tggaatgtca ataccagata cagcttttaa agttcaagaa aatgtaaaaa atactgttga
    24661 gacaatgaca ggactgaagg tttctcaagt aaatatacac attcaaggaa tcagctttaa
    24721 gaaggataaa gttgataaag aagaagcaaa agcaactaag aaaaattaat attagccctc
    24781 tgaaaagtca gagggttttt tgctggtaat tgattttata tggataatta aacttaaagg
    24841 agaattttga ttgatgaaaa aagatagggc acaaaagagt acaacaagag aatatataat
    24901 gaaacttata taccaaataa atattaataa agaagatttt gaaactttag aagataaagt
    24961 agataatttt ttaaaagata attcagaaca tattataaat agatataaag aacttgcact
    25021 tcaatactca aaaaatacaa atttaaagtt agaagatact gaaattgaag atgttataga
    25081 caaaaaatat ataaatacag tatgcaaagc attgaaagaa aatcatgaca aaatagatga
    25141 gttaataaat aagcatgcta aaaactggac agtagataga atgccaaagg tagatgtttc
    25201 tatacttaga ttgtctgtgt gtgaaatact ttatttagac actcctaata aagtatcaat
    25261 aaatgaagca gtagaacttg caaaaatata ttgtgatgat aagtctccta aatttataaa
    25321 tggtatattg ggaagtgttg ttgatgaaat tgggaaataa tataataatt ggaattgata
    25381 ctagctgcta tactacttct atagcagcta tctctttaga taagaaagtt atattcaatg
    25441 aaaaaataat gttagaagta agggataatt caaaaggttt aaggcaaagt gaagctgtat
    25501 ttcaacatat caataattta gggattctta gtgataggat aaaatctttt aaagataagt
    25561 ttaatgtaga aggagtttgc tcatcaaaaa aaccgagacc tgttgaaaac tcatatatgc
    25621 ctgtattcaa tgtaggtcat aattttggaa aattattgtc tagtatatat ggttgtaggt
    25681 tttatgagac tactcatcaa gagaatcata ttgaagcaag tttattaaat agtaaattaa
    25741 aaaataataa taaatttatt tctgttcata tgtctggtgg tactacagag atacttttaa
    25801 caagcaaaca agatagtcat cacaatgtat gtgatactaa tttaggtaaa attgccaaaa
    25861 taagtattaa aaaagatgat aaaagtaagt tatacaataa ttttggatat aatatagata
    25921 taattggagg aagtaaagat attagctttg gtcaacttat agatagagta ggaattaagc
    25981 ttggatataa atttccttca ggaaagtatt tggatgaaaa tgctttaaat tgtaatctta
    26041 aaatagaaag tggtctgaaa acttctgtaa gagatgggta tatgaattta tcaggattag
    26101 aaaatcaggt aaataagatt ataaatgata atggagataa tactaatcaa aaagaatata
    26161 tatctaaatt ggtattagat tcagtagtta gaaatatgtt taaatcttta gtgtatttat
    26221 gtgaaactta taatgtaaat gaagtagtat ttgctggtgg agtttctgca agtaagtata
    26281 ttcttagaga attaagcatg aagttaagaa aaaagcatat agaagcttat tttacagaac
    26341 cacaatattc aactgataat gctgtcgggt gtgctataat aggattgaat aactttttag
    26401 gagaaagagt atgaaactaa gagctttaga tataagtgaa gcaaactcat atattaaaag
    26461 aattttaata aacgacccaa tactttctaa tcttaaagtt aaaggagaaa tttctaattt
    26521 taaggttcat agtagtggga atgtatattt atcacttaaa gatgaaacat caaagttaaa
    26581 ctgtgtaatt tttaaaagta attttaatcg caatttaaaa ttagataatg gtgtaaaaat
    26641 tatagctaat ggatatatat cagtctatga aagagatggt gcgtatcaat tatacattaa
    26701 tgaaattgaa atagagggaa taggaaattt acacatagag tttaatagat taaaggaaaa
    26761 acttaataaa gaaggattat ttgaccctaa atacaagata cctataccta aaatgccaaa
    26821 ctcaattggc gttataacat ctccaacagg tgctgttata agagatataa taaatgtaat
    26881 aaaaagaaga tatccgaaag ttaatataaa gctttatcca gtaacggttc aaggagataa
    26941 atcagctgaa gaaatctgtg aagcaattag atttttcaat catatgaaga atgttgatac
    27001 acttatagtt ggtagaggtg ggggttcgat agaagaactt tggtctttta atgatgaaat
    27061 ggtagcaaga gaggttttta attctcaaat tccgataata tcagcagttg ggcatgaaac
    27121 ggattttact atttgtgact ttgtgtctga tatgagagcc ccaacgccat cagcagcagc
    27181 tgagattgct actccatcat tggatgatat aaactataaa cttggaaaca taaaaagtag
    27241 gatgagtaag tctcttacaa atcaaataga gctagaccaa tataggttag aaacagtatt
    27301 taataagata aataactatt tagattcgta cacaataaaa gataaagtta tacaattaga
    27361 caaaatatat gataaaataa tttttggaat agaaaataat ttaaaattag aagatgaaaa
    27421 attagtaaaa attggagcat tactccataa tttaagtcca ttagctacta tggatagagg
    27481 ttatagtata actcaaaaaa atggaaaagt aataaatagt ataaaaggat taaaaataaa
    27541 ggatagtata gatatagtat taaaagatgg aaatttagaa tgtatgatag ataaaattga
    27601 aaataaagag ggatagtatg aatttaacct acgaagaagc ttataaaaga ttagaaagta
    27661 ttttaagtga attagaatct aaaaatgcta gcttggatga atctttaagc ctatatgaag
    27721 aaggtataag cctttataaa cattgtaata agcttttaga tgatgctaaa ttgaaaataa
    27781 gtaaatttaa tcaattggga atagaagaag actttaaaat agaggaggaa taaaattgga
    27841 atttaaacaa tgtctaaaag aaaaagcaag ttttgtagaa aaagtactta aagaatatat
    27901 gcctaaagaa gaagggtatc aaaaaactgt tattgaagct atgaactata gcttaagtgc
    27961 tggaggtaaa agattaagac caatacttac attggaggct tgtaaaatag ttggaggtaa
    28021 tgaagatgaa gctattcctt ttgctattgc aattgaaatg atacatacat attcactaat
    28081 acatgatgat ttgccagctc ttgacaatga tgatttaaga aggggaagac caacaaatca
    28141 taaagtatat ggagaagcta tggggatttt agcaggagat gctttgttaa actatgcttt
    28201 tgaagtaatg cttgcaggct caatcaataa agaaaatcca gaaaagtatc taaaagctat
    28261 aaatgaaatt gccaaaggtg ctggtatata tggaatgata ggtggtcaag tagtagatgt
    28321 agaaagcgaa aataaacaaa ttggaaaaga aaaattagat tatatacata tgaataaaac
    28381 tgctgcaatg atggtaggct gtatgagagc aggagctact ataggtggag caaattctga
    28441 acaaatggaa gaaataacta aatatgcaaa aaatatcgga ttatcattcc agatagtaga
    28501 tgatatacta gacatagtag gagatgaagc taagttaggt aaaaaggttg gaagtgatat
    28561 agaaaatcat aagtcaacat acccatctct tttaggttta gataaatcta aagaaattgc
    28621 acataatttg attgatgaag caaagaaaag tatagaaaaa ttatctgatg atgtagattt
    28681 tttaaaggga ttggctgaat atataataga tagagaatac taaaatatac agctaaaatc
    28741 gaggagggaa aaatggattt tttttcggag atttttaaca atggggcttt gggaataagt
    28801 ctgattgctt gtttcttagc acaatttata aaaatattta ctggtaagga aaaaagaatt
    28861 gagttatcaa gaatacttat ttcaggaggt atgcctagtt cccatagttc ttttgttaca
    28921 agtttagcta cagtggtagg tattgaaaaa gggtttaatt ctactgattt tgctataatt
    28981 accgtgcttg ccttaattat aatgtatgat gcagcaggtg ttagacgggc tgttggtaaa
    29041 caagctacta tattaaatca aatggtggca gatatacagc atggaaagca tatagagcag
    29101 aagaaattaa aagaattaat aggtcataca ccactagaag tttggtttgg agctttactt
    29161 ggaatagtaa cagctttaat attgatgtaa attgaaaatt tacagaataa aggtgaggat
    29221 aatatgtata aatatttaga taaagtaaat tctccaaagg atataaaaaa tatgagtata
    29281 gaagaaatgg atttacttgc aaaggatata agaaagttct tagtaaaatc tgtttcaaag
    29341 acaggaggac acttagcttc taatctagga gtggttgagt taacattagc acttcataaa
    29401 gtatttgata gccctaaaga taagattgta tgggatgtag ggcatcaatc ttatgttcat
    29461 aaaatagtta ctggaagaaa agattgtttt gtatctttga gacagtttaa tggactaagt
    29521 ggttttccaa aggagaatga aagtcctcat gatatttttg atacaggaca tagcagtaca
    29581 tctatttcta tagctacagg tattgcctgt gctagggata taaaaaaaga aaactatagt
    29641 gttatatctg tgataggaga tggttctata acaggaggta tggcacttga agctttaaat
    29701 caattaggat atatagatac caatatgata gtaatcctta atgataatga gatgtccata
    29761 gataaaaatg ttggaggaat gtctaaatat ctgtcaagta ttataagaaa ctcaacagtt
    29821 gagaaaatga cagatgaagt tgataaaatt ttaaatgtta cacaaacagg tgaaatttta
    29881 tctaagactg cacatagatt taaagataaa ctaatgtata gcttttctcc tcaggactgt
    29941 tcattttttg attcattagg tataagatat tatggaccta tagatggcca taatactaaa
    30001 gaactaatag atatattgag aaaagctaaa cacaaaaaag ggcctgttct tttacatgta
    30061 attacgaaaa aaggaaaagg gtacagattt gcagaagaac aacctgataa atatcatgga
    30121 gtatcaaagt ttgatataaa gacaggtgtt acatctgcaa aagttaagtc tatgtcaatt
    30181 agtgttggag aaaaacttgt tgatatggct aatagtaatg aaaatatagt agcaataaca
    30241 gctgcaatgc catctggtac agggttaaac ctgtttgaaa gtgcatatcc aaaaagatat
    30301 tatgatgttg gtatagctga acaacatgca acaggatttg cagcaggtct tgcaaaaaat
    30361 ggtatgaaac cttactttgc tgtgtactca tcatttttgc aaagggcata tgaccaagtt
    30421 attcatgatg tatgcataac aaaaaagcca gttacttttc ttatagatag agctggatta
    30481 gttggaaatg atggagaaac tcatcatggt atgtttgatt taagttactt aaattctatt
    30541 ccaaatattg tagtaatggc tccaaaagac acaagagaga tggagcttat gatggatttg
    30601 tcattaaaat tagattgtcc actagcaatt agatacccaa gaggaagtag ttattaccta
    30661 gataagggag agtatggaga gattgtactg ggtaaatatg aggtattaga tgacggtcaa
    30721 gatacagtca tactttgtat tggaagtatg gtaaaacatg ctttagaggc taaagaaata
    30781 ttatcaagag aaggaataaa tccgacaata gttaatgcta gattcttaaa gccaattgat
    30841 gaaggtatgt taaaagcatt attaaaaaat cataaaaatg tagttactat agaggataat
    30901 attgtaactg gaggatttgg aagtagaata aataagttta ttatagataa tgaatataac
    30961 gtaaatatac ttaatatagc aataccagaa gaatttgtaa aacatggcaa tatagatgaa
    31021 ttatatgatt ttgtaggatt atcgccaaaa agtattgctg ataaaataag gaaattagtt
    31081 attgagtaaa aatctaatgt agatattgta aattaaaatt tatataaaat atagaattta
    31141 tcaattagaa taaatatcaa gtgtgttact tcaagctact ttagttatca ttaaagtagc
    31201 ttttaacaga aaggtaattg tatgaaaaaa agaatagatt tattattagt agaacaaggg
    31261 tactttgaaa gtagagaaag agccaaaaaa gcaataatgg ctggattggt ttttgttgat
    31321 aatcaaaggt gtgataaggc tggtacagaa gtaaaagaag attgtagtat agaagtaaaa
    31381 ggaaatccaa taccatatgt cagtagaggt ggtttaaagc ttgaaaaagc aatgaaaaac
    31441 tttgatttga ctattgatgg aaaggtatgt atggatatag gggcatccac aggtggtttt
    31501 acagattgta tgctcaaaaa tggagcaata aaagtatttt ctatagatgt tggttatggt
    31561 caacttgcat ggaaattgag acaagatgat agggttgtgt gtatggaaag aaccaatata
    31621 agaaatgtga caatagaaga tacaaagcag tttgctgatt ttgcgtcaat tgatgtgtct
    31681 tttatatcgt taaagttagt acttccaaag gcaaaggagt tagtacgtca tgatggggaa
    31741 gttgtcgctt taattaaacc tcaatttgaa gcaggaagag aaaaagtagg taaaaaagga
    31801 gtagttagag aaaaatctac acatatagaa gttataaaaa tgatttctga tttctcagtc
    31861 gaaaatggat ttgagatttt agggttagat ttttctccta ttaaaggtcc tgaaggtaat
    31921 atagaatatc taattcattt gagaaatgga aatgaaggat atgaatttga tggtgagaca
    31981 tacaataaca aaatagtaga agttgttgaa gcttctcata atttagacaa ataggttaaa
    32041 agggggctta gatttgatcc tcgaattata tatgaaaaat tgtgccttag ttgaagaact
    32101 aaggttgaat attgataaaa acttaaatat acttacaggt gaaactggtt caggaaaatc
    32161 tataataata gatgcacttg gactttgttt aggtgataaa tatgataggt cttttttaag
    32221 aaaaggaaca gataagggac ttgttgaggc tgtatttttt tcagacaata gatatcttaa
    32281 aaaaatatta gaagaaaatg atataagtat ggaagatgat aatttattgg taataactag
    32341 gttgatatac tcagatggta agagtactgc tagagtaaat ggaaggactg ttaaagtgtc
    32401 tcttcttaaa gaaattgcat ctacacttat agatatacat ggtcaacatc aaaatcaagc
    32461 tttgtttaat aaagatactc atttaaaatt tctagacctt tttggagaaa atgaattgga
    32521 agagtttaaa attgcttata aaaatgtgta ccataagtat agtgaagtaa aaaaagcttt
    32581 aaattgcttg acagaaaata aagatgagat gcaaattcaa cgagaaatag atttattaag
    32641 atttcaaata aatgaaattg aagctgcaaa tctaaataag aatgaatatg aagatttatt
    32701 aaaacaaaga gaagtttata gaaatagtga aaagatatat aataatctaa attcgagtta
    32761 tagcaagtta cataatggtg agtataatgt tattgaccta ataggtcttg catctaagga
    32821 acttaatgac atatctaaat atgacagtgt tttaagtgaa tatagtgata ctgtagaaag
    32881 aatcatgtat gagttacaag acatatctgg tgagataaga aattacaaag ataatataga
    32941 ctttgaacct tatgagctag agcagattga gcttagaatc gacgagatta ataacttaag
    33001 aagaaaatat ggagacagta tagatgatat atttgagtat tatggaaaaa ttaaagatag
    33061 attagatgaa atattaaaca gagatgaaag agttgaacag ttaagaagca aactgatgaa
    33121 tatagaagaa gatttaaaaa taaaagccag taaattgact aaagcaagaa tagaagtagc
    33181 aactacttta gaaaaaatac ttcttgatga attaaaaagc ttaaatatga aaaatgtaat
    33241 gttcaaggtt aattttgaaa aaggtccatt tactttagat ggagtagatg atattgaatt
    33301 tatgatatct tttaacttag gagaagatat taagcctata tataaagttg catcaggtgg
    33361 tgagatgtcc agatttatgt tagcgtttaa aaccatattg gctgatatag atgatattga
    33421 tacattagtg tttgacgaga tagatactgg aataagtggg atagcagcac agatagttgg
    33481 tgagaaattg agtgatatag caaagaaaaa acagataata tgtataactc atttacctca
    33541 aattgctgca aatgcagaca ctcattattg tatagagaaa gatacttcaa ataataggac
    33601 gtttaccaat gttagtaagt taaatgaatc tcaacgaaaa aatgaaattg ccagacttat
    33661 agcaggtaac aatataacag aaaaaactat tgaacatgca agtgaaatta ttgagttggc
    33721 taaaaaatgt tagtgaaaaa caagcatttt aataggtgta aagaattttt atagaaatgc
    33781 tttacatcta ttttttatga tgaaattatt ttaaaatatc tagtgcctat attgaagaaa
    33841 agtagattta aaaatagaag aaaatttgta taaatttgta actttgaaat ttctaaatgc
    33901 ttaagtataa tattatttat taaggtagta gtgaggggga cataaaatga ataaaaaaga
    33961 tactatgcac ttaattttta caattgtaat ttttatttta ttatggtata ttgtggtagc
    34021 agtaagtgag attaataaaa atattgaaag ttgtagtaat aaattagatt atttgtcaca
    34081 agagattatg agtactaaat cggatattag taatactata aatgaagaaa tgtcaaaaag
    34141 ttatataaca aagagtattg acttaaaagt aaaaaaaatc gagaaaaaag agtgtgttat
    34201 tgatgtagat gttcaattaa gtaaacttgg aaaaaatgga aaagtatttt ttatgtataa
    34261 agaagatagt gataaatgga atgaaactga gatgacaaaa catggagaat tatcatatgc
    34321 atgtgaaatt aagataaata caggaagtga atatgactat aaagtagtta caagtggagc
    34381 tatatctgaa agttctgatg tgcaaaaatt gactaaatct gattatatgc ctgaacaacc
    34441 catatttaat tctgggataa gagataatag agaatacttt attgaaatag tagaaaatag
    34501 taatcttttc aatcatgaga gtgaaaagtc aaaaaataaa gtttatgata atattagatt
    34561 agaaaaagta gatattattg taaatgaagg aaaaaaagac actatataca aagccaaatt
    34621 agctgaaggc ttagatgatg gcaaaactaa taattcaaat agaaatcaag tgaattatga
    34681 agtaagtttt cctaaaaggg atattgatga cataatttat attaaagcta aacttacata
    34741 caataatgga gtagaatata caaaggatat aactgaagat ataactatgg gattacaaga
    34801 tttagaaaaa taaatacata tttaaataaa tattcaaata gatttgttca ataaaataga
    34861 gtataaataa aactgtagta gttttactta tactctatta aaaaattata attttaattc
    34921 catatatcta gccccttcaa ttggattata agtataaggt tctatatcat aaaaaccaaa
    34981 cttttcatat aatccttgtg cacttttcat gcttggaaga gtatctaatc tcatgtgtgt
    35041 atatccaaat tttttagctt cttcaatgat ttcttccaat agtatttttc ctatttttaa
    35101 tcctctaaat tgatttctta catataatct ttttaattca catattttac cttcaagttt
    35161 ttttaaagca acacaacctg ccaaattttc atcaacaaat gccaatatca atgagcctga
    35221 aggtttttta tattttcctg gaagtgtttt taattcatta ttaaagtctt gaaaacataa
    35281 gtctatattt agagaatttg aatattcagt aaatagaagt tttacgtttt caaggttttc
    35341 taaatctttt tcttcaacaa attgtaaagt tatattttga gccattttat ccgccattat
    35401 ataaacatgt ttatataatg acttcacctc cttgatatat gtatattttt cttaatatca
    35461 attagatttt aataccatta ctttttcacc accatagtca agctctccat taaactcaaa
    35521 tccaaaatct ttataaagct taatagccat tttattgtct tcgaatatac taagatatag
    35581 ttcatcacag ttatattcat ttttaagatg atttacaaga aaatctaatg atgcttttcc
    35641 atatccttta ccttgatatt ttgaatcaat cataaatcta tcaagccaaa ctctatcatc
    35701 ttctaaacga ccatacatag caaatcctat taaagaattt tcatcatata ttccaacagg
    35761 tacccaagct gttataaatt tagattcagc aatagaaagt gcattgcttt ctatataatc
    35821 taactgactt ttatctacag acaaactaac taaatcatgc caattattta tgtctgcaat
    35881 tcttacattt aatttcaaga tattaccgcc cttcaaactt tttgttacaa tctaatacta
    35941 tcatattatg tattaaaaat cacttattaa gattttaaat aaacttttta aatgaataaa
    36001 ttctcaggaa aaaggaatta taaatacata ataatacagc ataggagtag atggatatat
    36061 gaacaaaata aataaaaatt tcaaaaatat ttggataata ttaggagctg tatcgatttt
    36121 taccatggtt ataattttta ataaaaattg taatgttaat aaagagactc aaatagaagc
    36181 ttttacaatt aagaatcatg ataaaaaatt tgtatatcct atgggaaata taattggagt
    36241 aaaagcaaat acagatggtg tattggtctt aggatatgag gaagaggatg tagactatat
    36301 tggaggcata caaatcggtg ataatatagt aaaaatagat aataaaagga taaaaaatag
    36361 tcaagatgta tcagagattt tgaataagat taaaaaaagt aaagttgaag tgacttttga
    36421 gagaaaaaat aagtataaaa cagaagtaat agagactaaa aaagaaaatg gcaagtataa
    36481 acttggatta tgggtaagag ataaaatatc tggaattgga actatgactt tttacgaccc
    36541 tagtatggaa aagttcaaag caatcggtca tgccataaaa gattcggata ctaatgaact
    36601 attaaagata aagcaaggat atatatataa gccagaacaa ttaaaaatag taaaagctag
    36661 taatgaaaaa gtaggtaaaa taaaaggaga ttttaatgac agcaatttaa tgggtaattt
    36721 ctcaaataat tcagagttag gtataagtgg taatattaca gaaaaccata ataaagagtt
    36781 taatgtagca aataaagaaa agcaattaat agaagttgga agaccgcaag atgtaaaaat
    36841 aggagatgca gtaattcttt ttgaggataa aaacaaaaat ataacaagct atgatataaa
    36901 aatagaaagt atagtatatg ataaaggaaa ttatagagat atggtaataa aagtagtaga
    36961 tgacaagtta ttggaataca caggaggtat cgtacagggg atgagtggag ctccaataat
    37021 acaaaataat aaaattattg gtgcaataac tcatgttttt agagataatc cgaaaaaagg
    37081 atatggtatt tttatagatg aaatgataaa attgtaggtg aggcattaaa aaattttatt
    37141 attttatcaa ttatctagga ggaatataat tttggagtgt cgaatatgct ttagagtaga
    37201 taattaggaa gcaattgtgt aaaaagttta gttttctgta ataagaagat gttttttaat
    37261 ggggggattt ttagtggaaa aaatcaaaat agttttagca gatgacaata aggatttttg
    37321 tcaggtatta aaagagtatt tgtctaatga agatgatatc gatatattag gcatagctaa
    37381 ggatggaatt gaagcattag acttagtaaa aaagacacaa ccggacttat taatactaga
    37441 tgtaataatg ccacatctag atggattagg tgtaattgaa aaattgaata ctatggatat
    37501 acctaaaatg ccaaaaataa tagtactatc agcagtaggt caagataaga taactcaaag
    37561 cgcaataaat ctaggagcag attactacat agtaaagcca tttgattttg ttgtgtttat
    37621 aaacagaatt agagagctag tttctaatag agttacacaa gttgagccaa aacctagacc
    37681 agttcaagag actcaaatga ctagaagtga ttttgttaag aatgtaggaa atatagagac
    37741 agaaatcaca aatataatac atgaaatagg agtaccagct catataaaag gatatttata
    37801 tttaagagaa gcaataaaaa tggttattga taatgttgag cttttaggtg cagtaacaaa
    37861 agagttatat ccaagtatag ctaaaaaatt caatacaaca ccaagtagag ttgaaagagc
    37921 aataagacat gcaatagagg ttgcatggag tagaggaaaa gttgacacaa taaatcaatt
    37981 atttggatat acggtacaca atactaaagg aaaaccaact aattcagaat ttatagcaat
    38041 gattgctgat aaattaagat tagaacatag tatggttaaa taaacaagac ataaaaagta
    38101 aggcttttta attaaggcat tggctataaa tgcgtattac aagcagcgaa acggtataac
    38161 cactagggtt ataccgtttc gctattttaa taaatataaa aattttcttt attatttgct
    38221 tactatatca atataataat tttattatac tatggatata gtatgtgtct ttacaagttg
    38281 taaactgaca gtggtttatt ttttaatata aatattgact ttgatgcagg taaactttgt
    38341 atttttaagc gtattatgga atatgttaaa taaaaaaatg atgaaatata gtattgtaaa
    38401 tgccaaagat gcaaaacaaa cttaaaacat ttattttatt gttaagtaat gctataatat
    38461 aatgtgattt taataatgat agtggaggtt taaatatgag agtcgaggcc cctataaaag
    38521 tagatcgaaa aaccaaaaaa cttgctaaaa gagttgaaag tggggaaata gcagttataa
    38581 atcatataga catagatgaa gttgctgcaa actctttagt agaagctaaa ataaaacttg
    38641 tcataaatgc ggctccttct ataagtggta ggtatcccaa taaaggtcca gggatattaa
    38701 ctgaaaataa tatactaata attgataatg ttggagaaga gttatttgaa gaattaaaag
    38761 aaggagaaac tatagaagtt gtagatggaa agatatatag aaaaggaaaa tttttaggtg
    38821 caggagaagt tttagacaag tatgaggttt cacaccaaat aaaggctgcc tatgagaatt
    38881 tagctgtaga attggataga tttatagata atactataga ttatgctaaa aaagaaaaag
    38941 gatttatact tggagaagta gaaattccaa aagtgaaaac taattatgct aataaacatg
    39001 tattgatagt tgtaagaggt caggactaca aagaagattt aagtactatg ctttcttata
    39061 tagaagaggt aaagccagta ttggttggtg ttgatggagg agcagacgct ttaattgagt
    39121 ttggatatac tccagatgtg attgtaggtg atatggatag tgtaagtgat gaagctttaa
    39181 aaaaggctag tgaaatagta gtacatgctt atacagatgg aagagcacca ggtcttaaga
    39241 gggttgagga attaggctta gatgcagttg tatttcctgc gccaggaact agtgaagata
    39301 tagctatgct tatagcctat gagtataagg ctgagttaat agtagcagtt ggtacacatt
    39361 caaatatgat tgattttctt gaaaaaggaa gaaaaggtat ggcaagtact tttttagtta
    39421 gactaaaaat aggttcaaag cttatagatg ctaaaggcgt taatttgtta tacagaagta
    39481 aattaaaaat aaagtacata tgggcattaa tagcaacagc attatttcct gtgttggtag
    39541 ttgcatcatt gtcaccagga gttcaacagt tcatacaact aatgcaatta aagtttagag
    39601 tattattaca gatgtaaatt ttaggaggat aaatatgcat attaatatga aatattatat
    39661 agtcactata ggggctatat ttatagcttt gggtataggc atgttagtag gatttaattt
    39721 aaataataat caacaattaa gtgaacaaca agcaaatata ataaatgatt tggatgataa
    39781 gtttaatatc ttaaaagaaa aaaatgataa acttgacagt gacttagcaa gtgtaaatag
    39841 agattatgaa gaggcagtta attttataaa taaaaatgta gataagattt tagctggttc
    39901 gctgaatggt aagagtatag gaataatatc tgctaatgaa aatgacgatt atacaaaaaa
    39961 tatagaagac ataataaata aatctaatgg aagcattgca tttaatataa tattaaaaga
    40021 aaacataaca aatccagaaa agttaaagga aatgtcaact aaactaggta cagatgtaaa
    40081 aaatgcaaat gatgcagtta attatataat tgacactctt aaaaaggaag atgcaagtga
    40141 cattttaaca tatcttcaag aactagatgt aataaagttt aattttattg gagatacata
    40201 tctaaaatat gattccgttg taatagctgg tggaaatgat gcaaaagaca gtacaaaaca
    40261 gtttgaaaaa atagaaaaat ttgtagtttc aaaacttaaa tcagagaata aatatttagt
    40321 agaagttcaa aatacaggag ttaaaacttc ttatgtagaa ctatattcta aaaacaaagt
    40381 tgcaactata gacaatattg atgaaggtat aggaagtata tctctagcga ttcttctaca
    40441 acaaggtaat atagttggta attttggtag attggatact gcaacttcat tgttgccatc
    40501 tattaaatag aaataatttt aaggagagaa aaaatggatt cttatataag tataataata
    40561 ccagcttata atgaagaaag tagaataaaa tatacattag aaagcatatt ggacattaag
    40621 caaataaatg aaataatagt tgtggatgat ggttcaattg acaatactgc taaggttgta
    40681 tctgagctag aaaatgaaaa gattaaattt tttaagttag ataaaaatag aggtaaagga
    40741 tatgctttaa attatggtat caagttagca atgaaaaatg cagatattat aggttttctg
    40801 gatggagatt taggaagttc tgctaaagaa gttgaaaaat taatagtacc tatattaaat
    40861 ggtgaggcgg atgttacaat agccaagttt cctcctgcaa agagaaaagg aggtttggga
    40921 tttgtaaaag gtttagctaa aaattctgtg tttgagatga ctggtgttga gttagatgca
    40981 actttatcag gtcaaagaat atttaaaaga gaagttttag aaaagtttga tgaaatacct
    41041 tttggatatg gagtagaagt tggaatgacc attgatatat taaagtatgg atttaaaatt
    41101 aaagaagtat tagtaaatat gactcataat gaaacaggaa gaaatttaaa aggttttatt
    41161 catagaggga aacagtatta tcacataaaa aaggttttag gacaaaaaaa gaaagaatgg
    41221 aggtaatatc ttagtgaaag attatatact ttataccatt ttattattaa ctggattatt
    41281 tggaacctat gctgtaattc cactatttaa aaatttattg ataaatggaa atgtacttag
    41341 accaaactat aaaaaagata tgataccagt aagtatggga attgtatttt tgcctatgat
    41401 tgtaatcaat ggaataatac ttgctttctt tactacagaa tttaagaatt tagcgtatat
    41461 atttatgttt atgtttggta tgatatccat gttttttgca gggatactag atgatgtcat
    41521 tggaaataga gatgtaagtg gtttaaaggg gcattttaaa agtttattta aaggaagtct
    41581 tacaacagga ggttttaaag ctttatttgg tggatttgta gggcttatag tttctgttgc
    41641 tatatcaaaa gacataattg atataatagt aaatacacta ataatagctc tatcaactaa
    41701 tttaatgaat cttttggatt taagaccagg aagggcaata aaagcatatt tattcataat
    41761 ggtaattgta tttttaacat tgacaggatt tgttcaagtt cttccattac ttatagtacc
    41821 aaatgtactt gcatacttta attatgattt aaaagcaagg gctatgatgg gagatactgg
    41881 ttcaaacgtc cttggaatat ctattggaat gctcatttct tttgggtatc catttaacat
    41941 tagattagga tggttaatat ttgtagttat tatgcatata ttcactgaga aatactcatt
    42001 aactaagata atagaaaaaa ataaactatt aaattttata gataaattgg gaaggtagta
    42061 ttatgataag caaaagagtg gggattgttg aatcaatagt aagtcaaact gaaactctag
    42121 atgatataag agtaaatata aatggagaaa tacaaaaggc atataattat ccaaaaatat
    42181 ctggaactat aaatataggt gacgaagtgg ttttaaatac aacagcagta gaactgagct
    42241 taggtacagg tggataccat tttgtaataa ctaatttaaa taatatagaa tctactttaa
    42301 ctgaaggtgg acatataatg aaacttagat atacgcctct tcaaataaaa gtggattctg
    42361 ttgaggaaca agaaagtatt tatcatgata aatttgcgaa ttttaaagga ttagaaggtc
    42421 tttctgttgt agtagggact cttcatagta tgttaactcc atttgcagct tcatttaaaa
    42481 gaaataatcc aaataaaaaa ttagtgtata taatgactga tggagcatct cttccaattt
    42541 atttaagtaa aaatgtggat actttaaaag aaaaaaaatt aatagatagt acaataacta
    42601 taggaaatgc gtttggtgga gattatgaat gtataaatat atatactgca ttaattacag
    42661 caaaagaaat actaaaagcg gatgcagtat ttgtcagtat gggacctgga attgctggga
    42721 caggaacaaa atatggattt acagggattg aacaaggctc tattcttgat gctgtaaaaa
    42781 aacttgaagg aagagcaatt gcaattccaa gaattagttt tgctgacaaa cgagaaagac
    42841 atcaaggtat ttcacatcat agtatgactg tatttaaaga aattgtaaat gtaaatgtag
    42901 acataccaat aactatatat gatgatgaaa aattgaactt tataaaagag caaatcaatt
    42961 taaatggttt agacaaaaag cataatataa tttatataga taataataaa acgaaagagg
    43021 atttagacta ttttaatcta aaagtaaaga gtatgggaag aaattatgaa caagatgagg
    43081 catttttcaa agctgcaagc acagcagcat attacttaat ggaggtttaa gatggtatta
    43141 gaagaaaaaa ctattagtag tgatagggtt tatacaggta aagtaattac tttaaaggtt
    43201 gatactgttg aaattccagg gcaaggatat caaaaaaggg agttagtaga agttggtgga
    43261 gcagtaggaa tagttgcaat cacagatgat aataaagttg tgttagttaa gcaatttaga
    43321 aaacctatag aaaagccaat ctttgaaatt ccagctggta aactagaaaa aaatgaaagt
    43381 ccaaaagaat gtgctgaaag agaattaaaa gaagaaactg ggtatagtgc aaaaaatatt
    43441 aagctaatac ataaattttt cacttcagca ggtttttcta atgagataat gtttgtatat
    43501 ttagcaacag gtcttactcc aggagagaat aatttagatg cagatgagtt tttagatgtt
    43561 tatgaaattg agcttgaaga agcatataac atggtattaa aaaatgacgt tgaagatgca
    43621 aaaacatcta ttggattatt attagtaaaa gatatgttta aaaattaata tttataaaat
    43681 gaataaccct ttgtcctaaa gcatattatt ttataaagga caagggggaa ttaaattgag
    43741 aagaacatat agaaaaaatg attttagaga attaaataga caaataatta ttataggatt
    43801 gttgtttatg atgtctattg ttgttgggtc ttacataaat aaaatattgc caggttctag
    43861 taacaatata ttaaacaata taaatcctgc tgtagagtat tataatctaa atataagcat
    43921 aaaagataca gtgatacaaa atttaaaatc agatgcaatc tttatgggct caatagctct
    43981 attaagtcta tttgtagtaa caattccagc agtgttggta gcttttgtat taaaagggat
    44041 gtcaattggt tatacaataa atagttgtat attggcatta aaatttaaaa gtataaaaat
    44101 gattttaata atattattta aaaatttgat tattattcca tgtgcaatca tattagcatt
    44161 aattagttta agttatttta aagaaatggt ttatgaattt aaaaagaaaa gtagaaaaaa
    44221 tatgcaattt ttaattaaga ggtatatact aaatattata attataatag cactatcatt
    44281 ggggttgcaa ttaatattaa atactgcaag tattggtatt attaagttcc tagctaaata
    44341 aaggagttta taatggatat tatagaggga tatatagatt acataaaaga taaaaaaaaa
    44401 ttatcagaga atacagtggc atcatacttt atggatataa aaaaatatat ggaatatata
    44461 aatcaaaaaa gcattaaact agtagatata gtagaaaatg atattataag ttacttaata
    44521 aagttggaaa aagataatgt atcaatagct acaatagcga ggatgatatc ctctataaaa
    44581 tcttttcatg attatttatt tttaaatcat atatgtacaa ataatcctgc aaaggatata
    44641 aaaaaaccta aaataaaaaa agaaaatata aatatactta cagaagaaga aattgaaaga
    44701 ctattaaatt ttccaaagtt aacaacacct aagttgatta gggacaaggc tatttttgaa
    44761 gtattatatg gtactggtat aaaagtatct gaattagtag agatgaatat agaggatatt
    44821 gacctagata ttgattatat atattgtaat tcatgttgta aaaatcaaag agtgatacca
    44881 ctttgtgata ttacaaaact ttacttagaa aagtatctaa aagaagcaag acctaaaatg
    44941 gctgcagaag gtgaaaaatc attattcgta agttcattag gtcaaaggtt tacacgacaa
    45001 ggtctttgga aagtcataaa aaaatattct aatctagcca atatagataa aaatataaat
    45061 cctacaatgc ttagacattc atttgctatt catttgttaa atgaaggtgc taacattgct
    45121 gttgtaagta aaattttagg aaatgttaat ttatctagtt tgcaagtata tttaaatcat
    45181 atagataaaa acgtgagaag agaaataaag gagaaacatc ctagaaatga tgttgatgta
    45241 gaattacaaa aagcagaagc taaatagact atatagcaaa ttttataatt tagaaggagc
    45301 atatttatat gagcagagta atttggattg taattgatag tgttgggata ggagcattac
    45361 ctgatgctga aaaatttggt gatagtaagg atgtaagtac ccttggaaat atatttaagg
    45421 aatatccaga tattcagatt cctaatatga gaaatttagg tataggaaac atagaaggta
    45481 tagatttttt tgaaagtata aaagaaccaa taggttgttt tggaaagtgt aaggaaatgt
    45541 ctcagggaaa agatactact acaggtcatt gggaaatgac tggtataata gttgacaaac
    45601 catttaagac atttgaacat ggattctcta aggagataat agaagagttt gagaaaaaaa
    45661 ctggaagaaa agtagtagga aataaaccag cttcaggaac tgtaataata gatgagtatg
    45721 gagagcatca aataaaaact ggagatgtaa tagtgtatac atcagctgat agtgtatttc
    45781 agattgctgc taatgaagaa gtaattcccc tagaagaact ctataatatg tgcaaaatcg
    45841 ctagggaaat aatgatgggg gataatgcag tagctagagt aattgctaga ccattcatag
    45901 gtaagaaaaa aggtgagttt gtgaggactt caaatagaag agattattca ctagacccat
    45961 ttgaaccaac agttcttgat aatataaaag aatcaggact tgatgtttta gcagttggaa
    46021 aaatagaaga tatttttaat ggaaaaggaa ttacagatgc tatccacact aaaagcaata
    46081 tggatggagt ggatgaaact ttaaattata tgaaacaaga taataaaggt ctaatatatt
    46141 caaatctcgt tgattttgat tctaaatatg gtcataggag agacccagaa ggatataaga
    46201 aagctcttga agagtttgat agtagacttc ctgaaataat ggctaatatg agagaagatg
    46261 atattttaat aatcaatgca gaccatggaa atgacccaac atataaaggt acagaccata
    46321 caagagaata tatacctgtt atgatttatg gcaataagat taaaaaaggt tttaatttag
    46381 gagtaaaaga tacttttgct gatattggag caactgttgc agatatacta aatgtaaagt
    46441 tgcctaaaca tgggtcaagc tttaaagaag atttatttta aactataaaa tgataaaaac
    46501 ttagcttaat aggatggaga gattggaatg tttgaaaaga tagcacaaag tagtaaattt
    46561 ataaattcta aaagtaatat aaaacctaag attggtttga tattaggttc tggactaggt
    46621 gatttagcaa atgatataga agaccctgtc acaattaaat atagtgaaat tccaaacttc
    46681 cctgtatcaa cagtagcagg acatgctgga caacttgtaa ttggaaaact tgaaggaaaa
    46741 gaagttattg ctatgcaagg tagatttcat tactatgaag gatattctca gaaagaagct
    46801 acttttccag taagggttat gaaagaatta ggagtcgaga ttcttatagt aactaacgct
    46861 gctggaggcg taaataaaga atttaaagca ggagatttga tgcttataag agaccatata
    46921 aactttagtg gaagtaatcc attagtagga aaaaatgatg acagatttgg tgctagattt
    46981 ccagatatgt cagatgcata ttcttctaaa tatgtagatg tagttaaaaa ctgcgctaaa
    47041 gaatgcaata tagatgtcaa agaaggcgtt tatatgtttt ttagtggtcc aaactatgaa
    47101 acaccagcag aagtgagaat ggcacaaatt ttgggagctg atgctgtagg tatgtctact
    47161 gttccagaag tcatagtagc ttctcactct aatataggag ttattggtat atcttgtata
    47221 acaaatatgg ctgctggcat cttaaatcaa ccactaagtc atgaggaagt aatagaaact
    47281 actcaaaaag ttaaatcaga atttatgaat ttagttaaaa caacggttaa aaatttataa
    47341 gttaaagctt attatagatt ttttgaggaa gtttataata aaatagttaa tgtttaattt
    47401 gaaaagaatt aataaaaagc tgtcttgata tagaaagtta tttctaataa aggacagttt
    47461 ttttataata ctacaacatt tgaaagtgtt ttaaagttta atatttattt aaattattat
    47521 aggttagaat ggagatttca attatgagaa tgtatgatat tataaaaaaa aagagagata
    47581 acctagagct tagtaaaaaa gagataaact actttataga aaactattca aagggagata
    47641 tacctgacta tcaagcttct gcactattaa tgtcgattta tttaaataag atgaataaac
    47701 aggaaactgt acatcttaca gaagcaatga tgaactctgg agacatgata aacctatctg
    47761 agatagatgg aataaaggta gataagcata gtactggtgg tgttggagat aagactacca
    47821 tagctcttat acctttggtt gcttcatgtg gagcaccagt tgcaaagatg tcaggtagag
    47881 gtcttggtca tactggtggt actattgata aattggagtc tataccaggt ttttcgacag
    47941 aaatggagat aagtaaattt attgattctg taaataagtc taaaatagca gtttgtggtc
    48001 aaagtgcaaa ggtagcaatt gcagataaga aaatatatgc tcttagagat gttacagcaa
    48061 ctgttgataa tatatcattg atatcatcaa gtgtaatgtc taaaaagtta gcttcaggag
    48121 ctgatgcaat tgtgctagat gtaaaaactg gtgatggagc atttatgaag acattagaag
    48181 agtcttttga gttagctaaa tctatggttg atataggtac tggtatgaat agagatacaa
    48241 tagggattgt aacagatatg gatgaacctt taggttttgc agtgggaaac tctttagaag
    48301 ttatagaggc aattgagaca ttaaaaggaa atggacctaa agaccttgtt gagctatgcg
    48361 aagtattagg agcatatatg ttggttttat caaaggtagc acatgatttt gaagatggtg
    48421 ttgaaaaaat aagagaatca ataaaatctg gtactgctat acaaaaactc aaagagttta
    48481 tagagaatca aggtggtaat aaagatgtag tagatgatta ttctttattc aaaaaatcaa
    48541 agtataaata tccagttaaa tcaactaaat caggttatat aagcaaaatt aaagctgaag
    48601 atgttggtgt atcggctatg attcttggag caggtagaga aactaaagat gatattttag
    48661 atttatcagc agggataatt ttagaaaaga aagtaggaga ttatgtaaat gaaggtgaag
    48721 ttttagctta catgtattat aatgatgaac aaaagctttc attggcaaag ggaagattta
    48781 ttgattccta ctctattgta gattcaaaaa tagaaaaaaa taaattaata tatggtattg
    48841 taactaaaga agagattaaa aagttttaga aaattttaga aaagtgatta taaaaggcct
    48901 aacatgtcaa aaataatgtt aggtttttta ttttttgata aactataaat tataccaaaa
    48961 atatatattt taaaacattt tttacaatat aaataagttt attctacaag atgattgaaa
    49021 attaaagtat ctagttttat aatagaaaat ggataaatat acataaggag tagattatat
    49081 tatgaacttt aaagaaaata gattgaaaat agcagtatta ataattgtta ttttgattat
    49141 cttagcaggt atatttgtgt ttatacaaat aggtccttat gataaaaata acaaaaaaga
    49201 tgtaattatc gacgttccaa gtggagcatc tgttggaaaa atatctgaca tattatatga
    49261 aaataaatta ataaagaatg aactattatt taaacttttg gtaaaggtta gcaataaagc
    49321 tccgtctata aaatcaggaa catacttact taatcaatcg tattcaaaca atgatataat
    49381 aagtctttta gtttcaggta aaatatatca ggatggtata aaagtgacta ttccagaggg
    49441 agctacatct aaagaaatta tagcaatgtt agtaagtaaa aatttaggag ataaagctac
    49501 ttttgaaaat ttaataaaaa aacctcaaga attttatgat aaattttcat atttgaagga
    49561 agatggaata acctctttag aagggttctt gtatccagaa acttattatt ttaattcaaa
    49621 gaaacaatca gaagaagata ttttaagcga aatgttaaaa gtatttgaca gtaaatacac
    49681 ggacaagttt aaaaagaaac aaaaagaatt aaatatgact cttcaagaag taatggaaat
    49741 ggcatcaata attgagaaag aagctgttct tgacaaagac agacctataa tagcaagtgt
    49801 gttttataat agacttaagg taggtatgcc tcttcaatca gatgctacaa tacaatatat
    49861 atttgaagag agaaagaaaa ttgtaacata tgatgattta aaaattgatt caccatataa
    49921 tagttataaa aataagggat tacctcctac accaatatca aatcctggta taaaatctat
    49981 tgaagcagca ttatatccag agaaaacaga ctatttatat tttgtagcta agattgatgg
    50041 tggaaataat tatagtacaa actatcaaga ccatttaaaa tacgttaaag aatataaaga
    50101 agcaagggat aaacaatcaa aagatacaaa agcaacaaat aaagaaaata caaaaaaata
    50161 aaccatgatt ataaaaagtc taacagggta agaataatgt tgggcttttt tattgaaaat
    50221 tgttattctt agatttataa taaagaaagt gtaaatatat ataacaatat gaatttaaaa
    50281 aataataaat taatataaaa cttggatatt tattatttta gggagtgttt ttatgagtaa
    50341 tatagttaat gatttggttg aagattatat aagagtgaca ttaaaagaaa aagaaggatt
    50401 tttaaaagat ttagaaattt atgcagagga aaatagtgtt cctataatac ataaagaagt
    50461 ttcagattta ttaaaagtct tgttaaaggt tcaaaaacca aagagaattt tagaagtagg
    50521 atgtgcaatt ggttattctt ctattttttt tgcaactatt ataggaaaag atgcagatat
    50581 aataactgtt gagagaaatg aaaaaatgat agaaaaagca aaagaaaaca taaaattagc
    50641 tggatttgac aataacataa ctatactaga aggagatgca gaagaaaaac ttagtcaagt
    50701 ccaaggtgaa tttgacctta tatttataga tgcagccaaa ggtcaatata agctattttt
    50761 tgatttagtt atagataaat taaaagatgg gggactttta gtatctgaca atattttgta
    50821 taaaggtatg gttgctcatg atgattttgt tgtaagaaga aaaaagacta tagttaaaag
    50881 aatgagaaag tatttagatt atatatgtaa ttgtgactat ttaagcacat cattggtacc
    50941 aataggtgat ggtgtagcac taagttataa agaaagtaaa agaggtgatg tagatggaat
    51001 taaataaagt agaactttta gctccagcag gtgatttgga gagattgaaa atagctataa
    51061 catatggagc agatgccgtt tatataggtg gagaaatttt tggaatgagg tctgcagcga
    51121 agaattttag taaggaagat atggcagagg gagtagcttt tgcacatgaa agaggcaaga
    51181 aagtattcgt aacagtaaat ataatacctc ataatgaaga ttttctacaa ttagaagact
    51241 atttgttgga actagaagag ataggaatag atgcagttat aatagctgac ccaggagtgc
    51301 ttagtgtcat taaaaaagta attccgaata tggaaataca tttaagtaca caagcaaata
    51361 caaccaatta tctaagtgca aacttctggt acgagcatgg aataaaaaga gttgtagtgg
    51421 caagagaatt atcttttgat gaaatatatg agataagagc aaaaacacct ttagatatgg
    51481 atatagaagc gtttatgcat ggtgctatgt gtatttctta ttctggcaga tgtttaataa
    51541 gcaactatat gactggaaga gatgcaaaca aaggttcttg tgctcaatct tgtagatggc
    51601 aatatcattt ggtggaagaa aaaagacctg gtgagtattt tccaatatac gaagatgaaa
    51661 ggggtacatt cttctttaac tcaaaagatt tatgtatgat agagtatata ccagagttaa
    51721 tcaaatctgg tataacaagt ttaaaaatag aaggtagaat gaagactgca tactatgtag
    51781 ctactgttgt aagagcatac agaatggcaa tagatgaatt ttacagagac ccagaaaact
    51841 ggaagtttaa tccaatgtgg atggaagaac taaaaaaagg tagtcataga cattttacat
    51901 caggttttta cttgaataaa ccaacaacag aagaccaaaa ttatcaatca gcatcatatg
    51961 ttagaaacta tgattttata ggtatagtta gagaaacaga agatgaagat ggtcttattg
    52021 ttgttgagca aagaaataaa atgtgtgttg gtgatgaaat agaagttatg ggaccatata
    52081 aagaaactat gtttactaaa attgaagaaa tgtataatga agaaggagaa gctatagaat
    52141 cagctcctca tcctagacaa atagtgaagt taaagctatc agtaaaagtt ggtaaagatt
    52201 atatgcttag aaaagttata gaagaaaagg ttgaagaata ataaaaaatt gataaaaata
    52261 gtattagccc cttagaaatt gtcaataatg aaattatcta aggggtgatt tcatgtcaaa
    52321 aaagaagact ccatttttga aaaaagtggg aaagagaagc tggtgtatat ttactataat
    52381 tttaataatt tatagtgttt taatttatag gctagttgat atacaagtat taaaaggtga
    52441 taaatacaaa caaagtgttg aatcacaaag cgttgagaaa gtcgagttaa atagtggaag
    52501 aggaataatc tatgatagaa acaacaagaa gcttacagat acaagtaaat ctcaggtatt
    52561 aattgttgaa aaagaaaaat taaataataa ctataaaatt ttggagctta ttaagaaagc
    52621 tactaagatg aatgatttag atatatataa agctgtacaa gagcaattga cacgccctat
    52681 aatacagatt cagactaaaa atatcgatat cgataagagt atgaaaaaag aacttgagaa
    52741 aaatggaata atggtagaag aaaaaactat gaggtacgca aaagatggtt tattatcaca
    52801 tacaatagga tatataaaag aagatgataa atcaggtcaa tcaggaattg aaaagagtat
    52861 ggatagtgta cttagaaatt caaatgaaaa gtatataagt gcttttaaag ctggagatgc
    52921 aggaaatgaa aaatctctaa acatactaaa gggaagtgta aaaactgttg ataataagga
    52981 taaagataga catctaaaaa ctactattga ttataacatt caaaaaaaat tagaacaaat
    53041 actaaataaa gaagaaaatc ctacagcagc aataatctcg gaagcatcta caggtgaaat
    53101 tcttgcaatg tgctctagac ctaattttga ccaaaatgac atatcaaaaa gtttaaaagg
    53161 aaaaaatgga gaatttgaaa atagagttat aaaagcgaca tatccaccag ggtctgtatt
    53221 taagatggta gttttatttt ctgcacttga aaatggtgtt atagatgaga actatactta
    53281 caactgtaca ggtaagacta aagttgggaa tactaatgaa atactgcgat gtaataaaag
    53341 agatggtcat ggtttccaaa acttaagaca agctttttct aattcatgca atcctgcatt
    53401 tttagatata gctatgaaac ttggaaaaga aaagatactc aaatctgctg aaaaattaca
    53461 tttatttgaa aaggttgata ttggtcttga tgaagaaaaa attagagaag ctccaaaaaa
    53521 catatcaata agaaatttag ctataggtca agaaaatata gaatttacac cacttcaaat
    53581 aaatcaaatg actcaaataa tagctaataa tggaacattc aaaccactat acttatataa
    53641 aagtcttgtg gacaataata tgaatacaat aaaaacttat aagtcttcaa aaaaagaaga
    53701 acttatttct ccttatgtgt gtacacaagt taaagaatat atgaagtctg tatctaggac
    53761 tggtacagct aaagacctaa aagatattga aggtggttgt ggagttaaaa ctggtacagc
    53821 tcaaagtagt ttaaataaaa aagctataga ccacggatgg ataacaggat tttatcctga
    53881 agaaagacct aagtatgtaa taactgtgtt agtagaagga actcaaaaag gaaataaatc
    53941 tgcaacacct atatttaagg aaatatgtga aagtataaaa taaattatgt actataaaat
    54001 ttaaggatat atcgtaactt tgattttaga tagaaaattt tgtctaatga tgtacgatat
    54061 atccttttaa aatatagtat taactttctt gaacaagctc tttttctagt tttttaagag
    54121 cttttttttc gattctagat acatatgacc ttgatatatc gagtttttgt gcaatttctc
    54181 tttgtgtctt atagccataa ggagttaacc catatcttaa ctggacaatt tctctttctc
    54241 taggagtaag aattttgttt agttgttcgt aaagttttcc aacttgaact tttagttcaa
    54301 cttcatcaag aacatagtta gcctctgtac caagtatatc taaaagactt atttcattac
    54361 acactactct ttatatgtta ttggtattac tggtcttcat gtaatttcaa atggaccatt
    54421 aattcaaaag aatctagttt ttgttcttta gacttataat aaactgcttt atcaattaca
    54481 gattttagga gtttattttt tccaagggaa tctgaatcat ggtaaagtga taacacagtt
    54541 ttaatatctt ctattatttt agaattgtct tttttaacag tactcaatct attttttact
    54601 gtcttaattg aatctttaat ttcttcagtt cttgaactta tattgttgga cctttcaata
    54661 aatgtatctt catcatatat tcctcgttct agtaaatcat ataacttatt tttttgcttg
    54721 cctaattctt ttaattcagt ttctaagccc tttaagactc gtttttgaaa ttctatatta
    54781 ctatctttat tatttttatc taagtctata tcagatgctt ttaattgata tttttttaca
    54841 gtgtcttcca aagattttaa aatggcagct tcaataaatt caaatcgaga acttttattg
    54901 caaccaggat gataacaaat tatatagtcg tggtctgcat atggtctata tactaatggt
    54961 ctaccacaat ttttgcaaat cactattcct gcaagtggat tttttatttg attaccatag
    55021 ctgacatgac catttttttt gagtatattt tgagcttttt tccatgttat ttcacttatt
    55081 aaaggttcat gctttccttt tgcctcaatc cattcatctt taggtcttag tttaactgtt
    55141 tttattttat ttggattttt agattttttg tagtcctttt tttgccactg aatgtagcca
    55201 caatatgcct tattttttat tatatttgta attgcagaat tgtaccaatt agcacctgta
    55261 gcagttttaa tgcctaagtt atttaggtat tttgatattt tactacaacc catatcttca
    55321 ttaatatata agtcaaagat agtcctaact acatcagcat ctttatttgg tacaagtatt
    55381 ctttcttttc taccagttgt tacagcatca tatccaaatg gagcagaggt tcctatgaaa
    55441 ttcccttctt caactgattt tattctacct ctttgcatcc ttcttgatat aagttttaat
    55501 tcttttcttg ccataaatgc ttcaaattct gaatactcct catcaaattc atctgttagg
    55561 tcgtatgttt ttctaggtgt gatgattttg gtcttacttt ctttgaatgt ttctaatatg
    55621 ataccttggt ctttcatgtc tccacgacca agtctgtcta agtccattac taagactgca
    55681 tcatatttat tttcttcaac ttcttttaga agttcaagca ttttgggtct atgtattata
    55741 ctttcaccag aaactaattc ttcttttatc tcaattacat ctaagttttg ttcttttgct
    55801 agttttagta gggttgattt atgtctactt aaagtttcaa attcgccttg tttttctgcc
    55861 tcttcatcag cacgggattt tcttaagtat atggcaacac tcataataag taatcacact
    55921 ccttatgcac tattatatag taaatcaata gagtttaaaa tatagaaaat aagcatgtca
    55981 aaagttaatt taacttttga catacttatt ttctataata ttaatgcctt ataatccttt
    56041 aatatcattt agattagttt ttgcatattc attatccggg ttaagttcaa gcgccttatt
    56101 gtaagattct ctagctttat taaattctga tttactcata aaaataacac ctcttaaatt
    56161 gtaagcatca ctagcgtcta ttttataagc ttcattattt ttatctagat attctaagta
    56221 tttatcaatt gactttaaag cctcatcata tcgttgtaac tctatataaa tatatgattg
    56281 tgtataatat gcaagtggat ttttatcatc aatttctata gcttttttga tatcaatagc
    56341 agctttatta taatccttat cttcatcttt ttctgtttct gacctagcga ttttgacata
    56401 attagagcta agagctcttt taaagtcttt atgccataat aatttatatt cattatcttc
    56461 gattataaaa tatctatcaa aatttgcagt atcttcttta ttttcagtga tatactttat
    56521 agtatcagtt tcttttattt taacaagatt tgtgtactct ttattagaat atttgtattt
    56581 ctctacttta ttttcttcac caagtttata atcttttagg gtttgaagtt taaataaagt
    56641 atcttgatat tcttcaaatt tttctagact gacactttcc ttactgtctt tacttaataa
    56701 attatatgct acttcgtagt ctttgatttc tatactatca aggtactttt tagctacttc
    56761 tgtagcactt tcttttggag cacatccaac cattgatatc ataattaaaa atgatactaa
    56821 tattgctgtt atttttttac tgattatcat aatataatcc cccttatatt gtctctcatt
    56881 aattttattt tatcaccata ttctaccaaa gtcattaatt taagagtgat atttttcatt
    56941 aatattatat taagtaataa taacctttta aatttatggt aaaattttcc tgaaaagtat
    57001 tatataattt aggtgaaaat atttaagaga aggagatatt atgaaaatta aaaaattatt
    57061 aacaataggg ttatctttat ctatatttat tgctagttgc cctatatctg taaatgctct
    57121 tgataaaatt gaatcaatta aaggtgcaga taaatacgaa actgctggta taattgcaga
    57181 caaacaaaac tatactacag ctatattaat taatgcagac agtacaatgg ctgatggatt
    57241 aagtgcaagt ggacttgcag gagctataaa cgctccaatt cttttgacta agaaaaataa
    57301 cataccaaat gctacactta aaagattaga aaaggcaaaa aaagtatata taattggagg
    57361 agaaaactct attgataaat atactgaaac tgttctaaaa ggcaaaggaa tagagattaa
    57421 aagacttcaa ggtagtgata gaattaagac aagctataat gtcgcaaaag aaataaactc
    57481 aattaacaaa gtaaataaag tcatattgac taatgcattt aaaggagagc ctgatgcaat
    57541 gagtgctgca cctgtagcag ttagagataa agcagctata gtattaactg atggaaaaag
    57601 tgttccattt aatacaactg gtgtagaaag ttatgtaata ggtggtactt catcaatgaa
    57661 tgacaaaata gttaaggata ctaattcaac tagattaggt gggacagata gatatgatac
    57721 taacaaaaag ataataaata aattttatag tggagtaaaa gaattttata tagcaagtgg
    57781 tactgattta gtgtatgctc ttgttggttc tactgttgct aaaaataatg ctattgtgtt
    57841 agttgataat gatagtaata aaagtgtatt gaaaagtaca actaaattaa ctgctatagg
    57901 aaatttgagt gatagtatat tacagcagtg tttaaatgta acaaaaaata taggcgattc
    57961 taatactgga agagaatata cagtagaaga tgctgctaat gcagtgaaaa aactattagg
    58021 tgtgaagaaa ttttattacg aacctaatta ttcagaagat gaagatttaa tatttctact
    58081 agataaaaat tttgattttt caggaaaaga gtactatgta tttgcttaca ttaaacaggg
    58141 tgcagagggt gatgttagat actgtgtagc taaaaatgat atgagcaaag tttatacata
    58201 tgatgttaat ggtaatatga aagtatgtaa tccagatgat tataaaaata aaggaagtat
    58261 aacagaggaa caagcaaaac agatagcatt aaaagaatgt gctaagagac ataaagtcag
    58321 tatcaataac ttagtagttg atatgataga ttttagagat gatatatata gtggtaatgt
    58381 ctatgctgtt aaaatcaatt caaaaaatag taatggtact tttggttggt tttttataga
    58441 tatagaaact gggaaagttt ataattaaaa ttagaacttt taaacaagtg tttttaatta
    58501 tataatttac cataatatgt tataatattt ttagcaagaa gatttaactt acaatctata
    58561 gagtggagtt catgtacaaa agattatcct cccaacgatt cgaagggagg tgagttcatg
    58621 gataactttt tgtttaatgt tttagctagt ttaacagcta gtgtggtagt ttacttaatt
    58681 agtaaactat tcaaaaaagc aaaaagccac tctcgcacaa agagtgactt acgagttgaa
    58741 tttaaattta tatttaaatc caaaaaataa actctgttta tgatgaactc cactctaacg
    58801 caaaatagat tgtagttctt cttgctttta ttataccaca aaacattata aataaaactg
    58861 ttgtttataa aaaaatgcaa agtaaaattt tttataaaca atgataaaaa ttttattata
    58921 gagctattaa cttattaaat atctgtaaat gagcagatat ttcataagtt aatatttatt
    58981 gtttacataa aatatttggt aaaaataagt aagttttata tgttataata gttgtagcaa
    59041 gaataataat ctaaagtggc aagcatgtca gctggtgtta tatcagttta tatttatgat
    59101 aaaataaaaa tctcccaaac gccaataaga atgatttaaa aaaataatat tttcacttaa
    59161 aaataatcac tctttatagg agtaaattat tttcttgatt ttattatact acaaattggt
    59221 acagatattt aaaaataata tattcataat attataaata gttttgcagt gagcgatatt
    59281 tttgataaaa tagggcttaa cagttgaaac ataaggcatt gagggtatat gataaatatt
    59341 atcatttgca ctactcgtgg ttcactgcaa atttgagaga attgtataga tgtaagtctt
    59401 ggaaatgctc aatttatttt ggggttttat attaactaag tggtatgtaa agaagtgttg
    59461 atatttatat tgtctatccc cgctagcttg ttttatatta actaagtggt atgtaaagtc
    59521 gtaaattacg accgtacctt tcgtaaaata acaatcgttt tatattaact aagtggtatg
    59581 taaagctttt tgagtatgag tacaagcata ggaaaattaa tagttttata ttaactaagt
    59641 ggtatgtaaa gaacaaaaaa gatacatctg taactaattt ttatactgtt ttatattaat
    59701 taagtggtat gtaaagacag atataatatc aaaataaggt taggactttt aaaagcatag
    59761 ccatatcagt aaaaatttaa ttatcattta aaaaataaat ttttatttaa agaatacaca
    59821 taaatatttc cagactcaac tctcttaaac tattttcata tcttttagta gatacctttg
    59881 ttgtttttta atctttagca acttgttctt gagtgaacct cttatttttt ctaagttatt
    59941 tcaagctttt caaaaaattc tctatttata ttcatcacac atataataca attaattcag
    60001 ctttaattgc caatatttag tttttctgta tctgataaac caagaatata atcagtatat
    60061 aaaccaaaaa ttttagcaaa tattattaac ccatcgtctc ttgttggtct ttcaccagac
    60121 tctattctat tcataacact tgtatttata tttgtttttt caagcaattc tttttgagaa
    60181 ctattcatat tttccctaat atatttaatc ctttgattta catccataca taattatctc
    60241 gaaaagtttc tgttttggaa atattgttat tgaaatatag caattaatgt gaatatatga
    60301 aaaagataca ctatgatttt ataaaaaaga agacatacat aaaatttata cttacaaagt
    60361 gttgcaaatg agttaggttt taaaaatgca tcaatttatt ttaagtacag gaaaggggaa
    60421 tattcaatta gagttgatat gttacctata aactatctaa gatacttgat tatgatatag
    60481 aaaatttttt tacaaattaa atttctaaga tagaaattta attaagagga tgatgaggat
    60541 gaataattta caagtaatat aaagaaataa taagagaatt ttaaatgcat agcaactagc
    60601 agatatatat gaaaatgatg taaacaatat ataaactaac ttcaatagaa ataaagagaa
    60661 tgtaaattat gttttattac aattcgagta tttgaaagaa tttaagaact aatctattaa
    60721 tagtcagttg atttcaaaac attctagtca attatacctt tggatcgaaa gatgtgcaaa
    60781 tagacactgc aaaatattag atattgataa aacttgggaa caatttgaca atttagagaa
    60841 aatatatttc aagattaagc gagagaaact aagctgtata gaagatgtat taatagaaaa
    60901 tttaagattt aaggatacaa attaataaag caataatatg gcattagatg taaaaataga
    60961 agttaaaata ataaaagatg ttgttatatt aaatataaca cattggaaaa aagaaacaag
    61021 tctattaaca gttaaaatag ctagaagtca aggtgattta agttatgtaa aatattttgc
    61081 taatgagagc taagagcttc ttaacaagag ttttggtgtg tctcttgaaa caaggttaac
    61141 aattaataaa agacgaatgg cagaagaagg cgtatgtaag tttaaaagag ataagttaaa
    61201 caaactagat actatgtaag aggataagaa acttatagaa gaatatgtta cttttgtaaa
    61261 ggatttagct ttaaaatata gaatataaag tgattttaag taaaaattgt aacaattaaa
    61321 taagtataag acaagtttat gagttcatat tttataacaa ggggtgatgt tatgaaaata
    61381 ctacaacaat ttaaaccata caaatatgat aaagaaaaaa atatagaata tgatgaaaaa
    61441 attatagcta atcaaaacac agaagtttat atagtatatc cacgtatatc tgatgaagaa
    61501 aataggagaa gatgggaaga gttcaatgaa gtagctaaag aagtagctat taatatagct
    61561 atgaagaaag ttgagacagt agaaagttaa gagaatagag aagttgctaa tacttagcac
    61621 tttctcatag aagaaaattg gacaactatt tttaaataat taggaataaa aaagttaaat
    61681 catatttgaa aaaaggcgat aaaattgttg agtattgtta ttagtaataa gaatatatta
    61741 actatgtatt aaaagaaatt ataagttttt agtaaatata gtatttaagg atattaaaac
    61801 tggtaaaaaa tatcatttag actattaaat aaaaaagtgc tggtcaaagt agcaacacaa
    61861 taaataaaaa aacttataga aattataaga taaaaagatg agatttgaaa tggtcgaagt
    61921 taatataaaa attaaaggtg aagtaagtga aataattaac tcaattagag gtatatttaa
    61981 tagctttgat gaagaattaa tattagatat aaaatcagag taagaatata aaggtagata
    62041 cgtagaaagt tataagtaat tcaagactta aattaatttc tagttttttt aatgcaaaaa
    62101 tttaataaat ttcaaaatat aataattctg acatctaaaa agagctatta aaaatgaata
    62161 atattgacta ttaagatatg aatagtgtac atatattaaa ttaagatatc aagtaggtta
    62221 attttatata atgatggggc tcaaattgaa gaggcgatat aaaaatgtat taatatatat
    62281 aaatataaga actataatac atattaactg taaaattaag tttatagagt tatatttaac
    62341 atttaatgaa ctgaaaaata aattagcaaa taatgaaaaa acaacagtaa taaatgagaa
    62401 taacaaagta ttttcaggat tagtggattt ttctaattga gctttaatcc tagtttgttc
    62461 aatttcgata tttctaatat tatcaattta tttgtaataa ttatcaattt ttgcgaaata
    62521 tttttgataa tttatagaac tttatatatt ttccttgaag tatttttcgc ccttattgtt
    62581 tggtttccaa ttttaaaact cataaactcc caataaattc atgtataata taccttaatt
    62641 tataatatat ttaagagttt atttcagtaa tttttcgaac gattttgata tatttcattt
    62701 atagttttca aaaacttata caaaaaagag tgtttcatct ttctaaacac tctttttata
    62761 agaatttgaa taataatttt ttaaaaattg ataatatggt tatttctttt tcaagtttat
    62821 agtttcctca tttccattcc aagatataat tagcttgtaa ctagattctc tatttgaata
    62881 aagacctggt ctttgtattt ttattgcttt ttcttgacct gcttctaaaa tttcattttt
    62941 tacaactaca actgcatctt caccatctat aaattgatag gaataattaa atttttgttc
    63001 aactacatct ttactatttt tatattttat ttctaatttt gagtgtcctt cagtagttac
    63061 aaatttagct ttccaatttt ctgattctcc aataaagaca tttttatcat tttttatatt
    63121 aagtatagct atcacagaga ttattaaaac tataagaata cctataaaaa attttttcct
    63181 catatcaatc cctcctttga ataattataa catgagaata gttattcaat actaatttta
    63241 aaaatattgt ggatttatat tctattacaa tagaatgtct gttataacaa ataaaaagta
    63301 aggattaata tgaattatag gttaaaaaaa tctgtaacaa ctattttatc tttactcata
    63361 atgacaatac tattaccaac aaatgcaagt gctagtaagg tagaagattc atctatgaaa
    63421 gtaatgtatt cagagaaaca agtaacagac atggacaaat tatacgatat ggctaaaaat
    63481 ggaattactg atgttaaaag ttccaatgaa aaaggtataa tcaaaaatgc aagaacagga
    63541 gagtccatat acgttgattc tatatcaaca actcaattat tagaagttaa acaatctaaa
    63601 aatttaacat caaagatata ttctaagaca gtttttttga tactaataca ataaagcctc
    63661 ttagaaaagc aagtatatca gaagatggat gggataaaac tggtggtgta aaagcatctg
    63721 gtacagttac atatacaata tcaaaaggag atggagcatt tcaatatatt tccgttagta
    63781 aagtttctgg aaactggtct attgcagacg gccagattcg tgtaacaaat agaagggtgc
    63841 atataaatca aactggattt atttctaatg agctaggaat taagcagcaa caaaagaaag
    63901 agccaacatc aaattctttc tcatataatg caccttctag ttggaaacca gtaggtagag
    63961 attcagttca atacaattgt gggtttgaat taattgcaac attagtaaga ggaggaagtt
    64021 cttgggcatt caatttcatt gttcaacctt aaaatatttt aagtgaattt tattaaaatc
    64081 aaagatatac atatacttta aaattagaga atgtaaatta atttgtgttt ttaaagataa
    64141 ttcctatgtt atagtaacaa
//
LOCUS       DAYASV010000007        60401 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYASV010000007 DAYASV010000000
VERSION     DAYASV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 60401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.007"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..327
                     /locus_tag="ACGI68_000486"
     CDS             <1..327
                     /locus_tag="ACGI68_000486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136944.1"
                     /translation="IEAVRRLYLKGYNAKEIADTLNKEANTVNLCIYRNCVDLKKIHE
                     ENRIIRKDTLKLLDRHNKTYINDGSLLKYNRQSYKNGKNGDIKFDDKRGSKPYDIPGI
                     YKKNIF"
     gene            694..1245
                     /locus_tag="ACGI68_000487"
     CDS             694..1245
                     /locus_tag="ACGI68_000487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136945.1"
                     /translation="MSDTSLLHSTLWMIITFVIGFTCVDTIARSWHGMSIIHDIIIII
                     ITIIIAFIIEIYTFDEPKFNPDFDLENKIVENIELNGSIDLNKLADFEFSKMYICSPE
                     APMEHILEDGVFTISHRPFNNDNNYLIFVNEENEVVKFTGLNKKYKILNSTFKEYARE
                     DAIFKFNKLEYKDKTIYELNYFS"
     gene            1291..1809
                     /locus_tag="ACGI68_000488"
     CDS             1291..1809
                     /locus_tag="ACGI68_000488"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136946.1"
                     /translation="MIFKVILIGLLCFLSIYNALCCQYRFLKKIPYWIIISIFVMIIV
                     NSIYINNKTEYNQDFNLENKIVENIEADATVDLNKLVNFNFEKVYVCKNSIRMIDILK
                     DKSFAISHKKIDGDSNYLIFVNKNNKVVRFTGLNKKYKILNSTFKIYSKKDAIFKFDK
                     LRYKDKTVYELK"
     gene            1869..2579
                     /locus_tag="ACGI68_000489"
     CDS             1869..2579
                     /locus_tag="ACGI68_000489"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136947.1"
                     /translation="MKNKNINLQKNNTKEEESVLRKIYMNLKKPFKFIFNKIRAIIED
                     FLDNPIVRISFLIVALILMICFLKFSWSELENKYENRYQSNFQLDFNTENEIVENIKD
                     NKSVDLKKIIDIDFDKVYICSSYSIVKEKVKDISFISYIEQKSDEETKEYSSFNILSF
                     NIFNKDKKDVDSKSPYNDKDYNYLVFMNENNKVIKFVPLDKDYEILDTNFKEYNNKNT
                     IFEFEVKETGEKTVYKLK"
     gene            2921..3175
                     /locus_tag="ACGI68_000490"
     CDS             2921..3175
                     /locus_tag="ACGI68_000490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136948.1"
                     /translation="MQISNKETHLLKIQEKVKNKSILFLEKNKRILFNEKGVDEHVSK
                     AGYGAGAVVLIILVVAFFSDSFQNTVLPALQNKIMSIFNI"
     gene            3175..3729
                     /locus_tag="ACGI68_000491"
     CDS             3175..3729
                     /locus_tag="ACGI68_000491"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFH0136949.1"
                     /translation="MFIKKIIDFFISFIYYPRSNEKVKLNKKNIVVLFVIEILLYYFL
                     KLRFDENTPIIKGLLYFQVLIYTSYIDYKVYLIPNKIHIIIIFISIIDINLLSSIKGL
                     FLFPLPYYLIAFIFGGIGGGDIKLLASSGFLIGYKGYYTLLPFFISFILEIVINYNKL
                     RNCKNLKIALAPYLSVGCFLTYFI"
     gene            3741..4526
                     /locus_tag="ACGI68_000492"
     CDS             3741..4526
                     /locus_tag="ACGI68_000492"
                     /inference="COORDINATES: protein motif:HMM:NF028286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcpC/CpaB family pilus assembly protein"
                     /protein_id="HFH0136950.1"
                     /translation="MDFKSKLQNRKVLAVITLILATIIGFGSPLYERMKSKSTYVRVV
                     NKIEKGNKISSNQIREYTTTDSKSLPNSIIKNKDDVVGKYAVVDMYKEDNVLSSKISQ
                     NPVAENDYLYNLNGSKQAMSITIKNFAAGLSGKLQTGDIISVIASDYGENKKTIKLPQ
                     LEYIQVLAVTNQKGADKKDDTIEKGNEDEQELPTTITLLVNETQAKILADLEEKSKMH
                     VTLVYRGTDKTAKKFIEEQDRVINLKGSEGLEQLNDSNNMETN"
     gene            4536..5306
                     /locus_tag="ACGI68_000493"
     CDS             4536..5306
                     /locus_tag="ACGI68_000493"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0136951.1"
                     /translation="MSNRIISIWGDPFSGKTTFSVKLAKELSTKKKNVIILFPDLFCP
                     TMNIILPGHAEKKSLGNILSSTIIDEDIILKNSIYLEKNDYISLLGYRERENITTYPE
                     YRENKVKDLIRTLLQITDYIIIDCSSHFVVDLISSISLEISDIVIRLMTPTLKSISYF
                     ESNTPLLIDKRFNFENHVKVLSNFKSDDSIEIVKDRYDIKYELPYNDELRTQMMSGEL
                     FYNLKSKKSYLYNDTVRKLTSVILNENKEVKGFKLWRK"
     gene            5310..6704
                     /locus_tag="ACGI68_000494"
     CDS             5310..6704
                     /locus_tag="ACGI68_000494"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFH0136952.1"
                     /translation="MGRNLLNNKFFNDKKNNKDFYSILDEVQEHMSRTYTKALDDREE
                     MIPYIRKYINDNSLYTEEISEDELIDKLISEMAGFGCYDKYLYKKIINNKYIVRDDVE
                     EINSNHWKSIIVKYKNGKKEHVKNCFKSPEHAENIVKRMLRKCSDIVFDNANPIVRGH
                     LDGNIRITAVGMGITDKDKGVAVSIRIVNPEHMSEKEYIEKGTASEEMFYAIKTFYKY
                     GMSICIAGPTGSGKTTTASLIVENTIEDNNRLITIEEDVREFDFVKYDRDAIPKNEVI
                     HLVTKHSHDSNKDIDQDKLIELSLTMNPDYLIVSEMKGKEADSAKKAANIGIPILSTT
                     HSNSATDTYPRLVDLCKEKSKMDYDTLLRGVIKAFPIVIFQKKLKDSLNSKRVMEIKE
                     TTIDFEGNIRENILFEFNEIEDLVIDGERYINGEFKKLNNISEQSQNILKLNNIPKKE
                     LDRLLNWKDGDKYD"
     gene            6697..7632
                     /locus_tag="ACGI68_000495"
     CDS             6697..7632
                     /locus_tag="ACGI68_000495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136953.1"
                     /translation="MIKNIIIFMFVVIAMLELFDISREDIKKRILYLFNLQKKEYTIK
                     DKIKMANLEKKENTIVLLIKETRKILKSTGRTTMFSTICLTSVFLSVVGIFLSLLIGN
                     IFLVPILAIGFFMIPFQYIKISEGHYKSQISHNLESALFSITNTYMRTSNIIAAVQDN
                     IEEFEPSIRKEFEFFLIGVGLEPDVKKVLVKLKDRIDNDIFKEWIKRVIDCQNDNVLK
                     SPLKGIVKKFSKVKVLNKKCENELIEPIRNFYTLVIGCYSLFGIFYFLKRDLFDILMF
                     TNLGRICVAIAVLMTFICIHGVLRLKKPVDYKMLK"
     gene            7648..8511
                     /locus_tag="ACGI68_000496"
     CDS             7648..8511
                     /locus_tag="ACGI68_000496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136954.1"
                     /translation="MSLLITIVLLAIAIYLILAEILNIPTYRNNKIMGKVLKMNKDGK
                     YIDNLTTHMAIKLSKFIKISEERKEKISYLLKVSGIRLSPETFIANAFVSSIIMLIFF
                     IPTYLIMPSVSVIWIILAIYAFRVEIKKPYRLLKEKKRKIECNLADFSATLAIELEHT
                     RNLVRIFEGYKEFANPDFAEELEITLADMKSGSDKQALKRFDTRIGSSYLTEVIIGLI
                     GVIDGNDEVSYFSNLSDKLEKFEFEELSSIALGQKSKVAKYEIIMLTVIVMIILVAFA
                     SLFLKYSKELF"
     gene            8527..8943
                     /locus_tag="ACGI68_000497"
     CDS             8527..8943
                     /locus_tag="ACGI68_000497"
                     /inference="COORDINATES: protein motif:HMM:NF025571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4320 family protein"
                     /protein_id="HFH0136955.1"
                     /translation="MKNLLKDERGFIDSTVGVILIAIVASLFISLGFVIFPALDTQVQ
                     LNNFAKEIVREAQITGEIDKDIDKKIKNLEDNLVKPDKIIWDNKYITGTKKVQLNEEI
                     KVRVEKTIDIGFFDFGRFPVPLHGSHSGRSEVYWRN"
     gene            8931..9464
                     /locus_tag="ACGI68_000498"
     CDS             8931..9464
                     /locus_tag="ACGI68_000498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136956.1"
                     /translation="MEKLRDEKGAVNIVILGVVLVLVLIIGLYFEKIRLQIVAFGVRN
                     AFETSVTDISTENYDEVYPCIREGYSAAYKLDKSDKWEQNIDKGNVVEQLKKSLGLVE
                     EEGQLIKYNGDKIEFKLSDIKINILNRQLAPSDKTKLEKYRVNGQLTLEIPSNYNGKT
                     SYAKKDINVSSAYTSKY"
     gene            9559..9813
                     /locus_tag="ACGI68_000499"
     CDS             9559..9813
                     /locus_tag="ACGI68_000499"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0136957.1"
                     /translation="MPYKPSEFAQKLGISVKTLMRWDKDKKLIAFRTPTNRRYYTEEQ
                     YLKYVKDSGEKKILIFDCKDDIKSEKLRKYAKSIGCEEVN"
     gene            9839..10369
                     /locus_tag="ACGI68_000500"
     CDS             9839..10369
                     /locus_tag="ACGI68_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136958.1"
                     /translation="MKKLNINIKKKTIIMCSIVLTMCMVLLLSLLLMKQKKEIPVAKE
                     YKKEHETDIKKGDEINVPKIDTSKESITIGELNNTKSKDEKFENKGKDIDKKINNNSK
                     GENGTKEEIQKKTRPIKRPSDAKDEVIHNSNNQGSEDISENEESNNNEEVLNPGNIQE
                     EVKMEGEWGNIIANMR"
     gene            10414..11439
                     /locus_tag="ACGI68_000501"
     CDS             10414..11439
                     /locus_tag="ACGI68_000501"
                     /inference="COORDINATES: protein motif:HMM:NF016047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0136959.1"
                     /translation="MKIMKRILSLGLTVTFLVGSSTLVSNAMQLKDIDKIVGKNRYET
                     AGLIADKQKYTTAIIVNSDKSIADGLSASGLAGSENAPILLTQKDNVPDETLERLNTV
                     TKLYIIGGEDSISTDVEQMLTMRRMEVKRISGKDRIETSYNVAKAIDSINKVKNVILT
                     NAYKGEADSMSVASVAVRDKTPIILTDGNNIPFNTGGLKTYVIGGKSSMSDNLVNKTK
                     SVRISGIDRFKTNKEVISYFYKDVKECYITKAYTLIDALTGSTIAKNKPIVLADEVSD
                     KSVLKGMNKFDVIGGVNNTTINRCLNVLNGEEEKNNPQSSDDNKTGNIQDEIEMEGEW
                     GNIVADM"
     gene            11510..13273
                     /locus_tag="ACGI68_000502"
     CDS             11510..13273
                     /locus_tag="ACGI68_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136960.1"
                     /translation="MKKTITFLCIFLIMPILVFGDGNGNVDTGGGNMGGVVKGYKWTP
                     GEEGVRVTVIKDETKQPVTTPIDFTNVKNPNVKYHFGKVSKISYKNGTKLTRYNTATK
                     GYVSKRPSSGNIPIIIKSNNRKPDMKKIKAYFCDKVFIQTLVKLTGMNYEELIGGEYK
                     LLIEPIGYCTIKGERYALTAHEAALYDEQLKGTLRSKMPSFTHKNLPFALFLENADLG
                     FPAWKGTTNNKQLNSTIKDKLGLGIIYFGNESEEEKPPDIDIKPDPTPTPPKPNEDEG
                     NTDTKEYEYRTDTDVISSVLISGRNRTPDHPVNVSFKIKDKTYTMKDIYLPKEGGEQY
                     AWVKWHTPKTPERIIITVSSNATSETSKIIANVNNLEEKTPPDPTANDKKPDNYKVPP
                     KPENQTTSTEYGEWDCYWVSHWVYYSDGEGGGEWVDEGWWEWKWIPYKVTLSAKMNLK
                     ADSNVPTARNNGKEIKSGYGVDTVVDSKVGYNAPENSLTGAQNVIVTFPEFSYEKYNR
                     VLQNTKIGYDSSFVFKNNKYSTYNSKVHFTPLWFLDGKYITNSKVMDVWTPTGMLSIN
                     TNDYVDIKNNLYDDWHIGPVK"
     gene            13375..13563
                     /locus_tag="ACGI68_000503"
     CDS             13375..13563
                     /locus_tag="ACGI68_000503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136961.1"
                     /translation="MKCTGKRYFYIEKKDIEDKIDYITKSKEKLKKQFNLNDEDILKI
                     LESLQIKLEKINKKISKM"
     gene            13612..13776
                     /locus_tag="ACGI68_000504"
     CDS             13612..13776
                     /locus_tag="ACGI68_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136962.1"
                     /translation="MINNIEKVVSLGMIQFVTILLLIGYYFGGLKYTLILGFFIMFLL
                     GITIIKTSLN"
     gene            13796..14077
                     /locus_tag="ACGI68_000505"
     CDS             13796..14077
                     /locus_tag="ACGI68_000505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136963.1"
                     /translation="MNGDKNIKFTKVDEKRLEKRKKQKEIQKGIYLMGEYEEGKVRVS
                     TNLPISVADALIYYTKTESAKENSRSELLENIILDWTKENISEELLKEG"
     gene            14084..14245
                     /locus_tag="ACGI68_000506"
     CDS             14084..14245
                     /locus_tag="ACGI68_000506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136964.1"
                     /translation="MIDIESVFVDLVSRGRKWSVGLYIESETYERIINEESDKEVNVS
                     ELLKVLNNK"
     gene            14259..14900
                     /locus_tag="ACGI68_000507"
     CDS             14259..14900
                     /locus_tag="ACGI68_000507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136965.1"
                     /translation="MRYISMNKNGKKEFGKLISVMSDKDCNKIEIMLSVMDELSRDNT
                     ILYLSEKDICEETIFINYDYEKLEFQISIKDNFYGYSLLSFYRLEDMLRYVIKSIDNI
                     DIIFVDGTSFSVKVNYKKLKDLAKETGVTIILLLEKETIELLNTLKFRKHIKDIDESL
                     ILYIDRYKEIPEGYFYKVSGDEFKSIQINTPTWIEDVLKITGLGYKYVKVVDF"
     gene            14927..15202
                     /locus_tag="ACGI68_000508"
     CDS             14927..15202
                     /locus_tag="ACGI68_000508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136966.1"
                     /translation="MIIKSIIFLLYLFSICIYLIGIYSVNLKIKSNKENYELIKQCKK
                     HYIYIVREMAIGFIPIVNFLIGVMLIKIIMKMDDSEIKKVIENRKNR"
     gene            15397..15780
                     /locus_tag="ACGI68_000509"
     CDS             15397..15780
                     /locus_tag="ACGI68_000509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136967.1"
                     /translation="MYFILYIFLYIFSICIYSVGMYSINSKIKSNKENYELLEQGKKG
                     SRYIKKLIQAGAMPIVNFCIGVMLIRASVKMNDSEIKKCLEDANKINDVKVYDNQVKG
                     VYYIEYNIKDNKGLKSKGIVKAHVK"
     gene            16381..16539
                     /locus_tag="ACGI68_000510"
     CDS             16381..16539
                     /locus_tag="ACGI68_000510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136968.1"
                     /translation="MAIDKQIKSLAISILEKNNIDYDDWINEEHKKLIFSNTTVLQKA
                     IEESKKND"
     gene            16566..16796
                     /locus_tag="ACGI68_000511"
     CDS             16566..16796
                     /locus_tag="ACGI68_000511"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136969.1"
                     /translation="MEGNVVNLVNDLIKSGQLILAAVAAFCYLVGAYHQISGGKEGFP
                     VAKSWYKNTTFGLVIGMSVMQLVSFLQSKINF"
     gene            16886..19060
                     /locus_tag="ACGI68_000512"
     CDS             16886..19060
                     /locus_tag="ACGI68_000512"
                     /inference="COORDINATES: protein motif:HMM:NF045973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFH0136970.1"
                     /translation="MNLFENIKKNYNEFLDTPNAEMKLAKKLVNKEYVKKIFIVGLVA
                     ITLILNIAIELITSVLGILSGYKINIFRIFIPRWITCFPIYFIVYLLFIIVTTKMIVN
                     LRISFNKLEEGQKGTARFATLEEINAQYRSIPKRDIPFEGLGGIPIARNLVKDEIYID
                     DSIVNNLIIGMTRSGKGETFILPSLDIYSRAKNKISLVVNDPKGELAIMCKKIFEVRG
                     FDTYLLNLIEPMNSMSYNPLQLVIDAYKKGEYDEAQLLCKTLTHSIYYNPNSKDPIWE
                     KSAMSLVNALILAICDKCISEGSEEKITLYTVANMLSELGSKNYVDIYDVPHNALDDY
                     FQEMPNRSVAKMQYSTSKFSAGNTRASIFTTAMSELQVFTFNKLAKLTSKTSINFEDV
                     ASKDCKPSIIFMATPDYDTSNHFVASIFVRQLYYVLAKKATFSKGGKCERRVKFLLDE
                     FGNMPSIEGMANIITVCLGRGISFDLVVQSYTQLKSLYGDSMDTIKSNCGNKIYILTD
                     DPSTLEEVSKTIGEKTIITNSRSGEVFSTSKSQTESLDSRRLLSPDELAKLEKGEMVV
                     IRPIKREDINNEKIKAHPIFNQKERAMQHRYEYLLDTFNKEGLTLEDVKIDTLHKNVN
                     VEDLLIDFEETSVELDEKIENLLKKKLNPEEETLKKYNEREIDDIDLSDSEKYKIIRI
                     CKEWDVDSSDIEHIKNLNKKIDIYNYLETIGSGYLSDLIEES"
     gene            19148..21172
                     /locus_tag="ACGI68_000513"
     CDS             19148..21172
                     /locus_tag="ACGI68_000513"
                     /inference="COORDINATES: protein motif:HMM:NF045890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFH0136971.1"
                     /translation="MDEVKIIEILVNFSPVMQISDILSSLTRTIGWWLINALTFISGG
                     METVVDKIYSSMDFFNSEGISQLITQFKPLIWATLGVSILFVAYQLMINRKFQKDGLL
                     VNVLLSIMVIMMLPSLMVDLNKITKSAIKSVNSPYKTTAEQIIKDNLTDLYYLDKNNF
                     DSLENKNNIPEELVSKIFINEEVEDGKVENKDVFKNKLNIDTDGEKKVTKLGKVFGVF
                     SEKYYRYHLNFFIIIITLACTTVTQLFTAIKVAQLIFELAFTKLFATMYAFADISSGQ
                     RLKDIVKNILSIFAVIFSTALLLKLYLLYSNWITTIDNDNIFLKIFLMIGGAFVVIDG
                     PNIIERTLGIDAGIKSGLGVVTGAYSSMKMGGELAKGLGKATGFVASATAAGTGIASG
                     AYSEMKDLKSNNGNGEENNSSTGNNKGGYFGADKDNFKDETGSPGEKFSKEPDDLEQE
                     MKNSNPIDIDTMLEKSNGEDMVDGSIPQNDILDGELDKYEDLNNKGMNDNLQQDNIDS
                     NMEKDMQNFKLDTDEMSPLQSKIASGINRNRANFGGGDIEDIKPYEDFGRMTGKVNLP
                     NGGLNDDKDEIDIGNINLGSEKSKINMTQDVNDFNEEIKTSGINIDERINGNKGNISD
                     ETYGKTLGDSIKNNQTVNKIKTNYDVGKNTGKKFAKDINKISNNIKNYKK"
     gene            21219..21596
                     /locus_tag="ACGI68_000514"
     CDS             21219..21596
                     /locus_tag="ACGI68_000514"
                     /inference="COORDINATES: protein motif:HMM:NF036438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5592 family protein"
                     /protein_id="HFH0136972.1"
                     /translation="MYYIPEEIRSETKIFKSLYLSDLAFLVATLLLAWILSSIVYSPL
                     VIPYYIFTIVSTLFLLAKSSDNPGSRNYKTIYFLLTKSRHVYHIENNAETKIQLINYE
                     EFKKKIEKGEKYICQKEIRMIKE"
     gene            21563..22168
                     /locus_tag="ACGI68_000515"
     CDS             21563..22168
                     /locus_tag="ACGI68_000515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136973.1"
                     /translation="MSKGNKDDKRIKKEEKAFLKAEKKKKRKVEKNIAKLVSDLIPIL
                     DINVESESIVTNYGCMDIFQISSKDIYSLNEDESNAHIATFARFLRTYQSPIKFIFMN
                     FPIETTTQQEFLIKQIKKCKNETYEVLLREKLGQLMYLERDKTNKEFFMAIYVDNEKQ
                     RYEKEHMILRISDKFLEFKKISPAKKKKILFKLGNQNTKII"
     gene            22184..24100
                     /locus_tag="ACGI68_000516"
     CDS             22184..24100
                     /locus_tag="ACGI68_000516"
                     /inference="COORDINATES: protein motif:HMM:NF040179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFH0136974.1"
                     /translation="MRKNIFKKDNLNPFFLNDIQPQGGISFKDRVIVKGDGYETCIYV
                     YFYPGQVNEFWLNSLTSLSSLDNAIITIDVGTEDKQKALESINKSLIEQESRYYEDKD
                     KISQIKAKNTYGALHTLADNVTNQGEAIKLIQVRFYISDKEKDSLENKVKDIMQELEG
                     IGFRGTVCLNELEYDWQSLYYGPIEQKKFKNKRKGKAVPASSLAGGYPFHFTSLNDPT
                     GILLGQTFTGGNVIFDLFTKTDIRKFYNAVLFGTMGVGKSTTLKKLLLNNAIIGNIVR
                     LLDITGEFKPLITLLGGRIISLDGTGEIINPLQILSTMIDEETFEVLNKESFMMHMSK
                     MSMMYNYIAINPEVEEVREFDKLLSKFYNHFGIEIEKATEYESKEYPIMSDLLKFAKE
                     EMYVDVKKEEIRKNLTIDKQRRLENIILNLESIVRDYGALFDGHSTITDITNEQIISF
                     QLRNLSQYDKRIFRTQIFNILTLLWNNALKIGTKEKKSFDNGLKTLEDIMHYLIIIDE
                     AHTIINSENIMAVDYLITCEREFRKYFGGLLYATQSIRDMVPETIDSTSEVFGKIKTL
                     FELTQYKFIMQQDSNAMASLRKIFEGQLSESEIKSIPNLGMGQCILSINGMSNIKFKV
                     LATEEEKKLFAGGA"
     gene            24103..24684
                     /locus_tag="ACGI68_000517"
     CDS             24103..24684
                     /locus_tag="ACGI68_000517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136975.1"
                     /translation="MNINDNKKKTYTLFIIVVLMFFLYTFLSIKEKSIDEKQNKDIVE
                     VEQNENKDLNEENNKLLNEENNIEEKSVVDIGNKFIKAYHENLNSVEERLEALKSITD
                     VDLYESIENELLMDRNKGNGDYQHRVIKQIHKEEYESQSIESVLYKVKVYSNWLNKDN
                     KVTQEDITRYSIILVKENDKWIVADINSEILSK"
     gene            24693..25811
                     /locus_tag="ACGI68_000518"
     CDS             24693..25811
                     /locus_tag="ACGI68_000518"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0136976.1"
                     /translation="MKCKKSNILVKLLLASGVGTLLFFLFLISTIILLLSSSNRGGGF
                     QNATLKGVPEEFIQHFNEAAEVFKVPNWTLAAIAKQESNFNPSCAYGGAWGIMQIQKT
                     DWSYYIGNGLGDIYKELGYSYSSADDMWSTYLNDVRAQILAGSWECMRYTNVVLKNKG
                     IISKVPYKDNKKLAENMGLIDWYADENDSQFREILRRSFACYNGGPGYGLSVNLDTAQ
                     NDYPNKVFGYAMEFRNTGLVGDIIGGDVAVSDSEKVNAIISLMQQQLGKPYLWGATGP
                     NRFDCSGLMLYCFKNAVGINLPRTANAQYKASQPISFEELQPGDFVFRLSNGVAKHVQ
                     LYIGDGKVIHSPQTGDVVKISNVPHKSDNRYGRWSGLD"
     gene            25873..26097
                     /locus_tag="ACGI68_000519"
     CDS             25873..26097
                     /locus_tag="ACGI68_000519"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136977.1"
                     /translation="MKNIIKEKLNDSEFICKGGWKSGNTIIFDDGAERNIDYKEIENT
                     EYVSIILTPKDIEKLNIELKTKCYKYEYDC"
     gene            26149..26586
                     /locus_tag="ACGI68_000520"
     CDS             26149..26586
                     /locus_tag="ACGI68_000520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136978.1"
                     /translation="MEEYYIKCLNEEVDEIRFLYKNFAINLNVSENILDRKIVYVKSG
                     AILIGALYGFVRQRNEYSDEYKIFKEIEKILDSDNVINLEKSNVYFEQIKGMALTLWK
                     IYLKNAKTEIMRTLIVKELKSNLKDFYYEYSLKINMNKMYKLD"
     gene            26658..27431
                     /locus_tag="ACGI68_000521"
     CDS             26658..27431
                     /locus_tag="ACGI68_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0136979.1"
                     /translation="MNIFNFKRNKKNGRGVEVINEESNNRQTILELKKEKINLRKEKI
                     NTICLTKKEFINIEKTKFDVVVVLDCSGSTSNLFKNGTIQNILEKLLPISLKFDDDGE
                     LDVWLFNTSAYQFKSINIDNFFNYVEKENLYSEVGGGTYYSPVIKEIISKKIDKEKTN
                     NPVYVIFITDGDNFDKKETKQVIIEASNKPIFWQFVGIGREKFEFLESLDTMTGREVD
                     NANFFSVNNIEVIEESELYEKVMKELPIWFKEIEKKRIL"
     gene            27456..27830
                     /locus_tag="ACGI68_000522"
     CDS             27456..27830
                     /locus_tag="ACGI68_000522"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0136980.1"
                     /translation="MNDVSLIGRLTSHPELKCKANGKYVLYFNLAVDNMKSTDFIPVE
                     VHGILVEKYSTELEKGHKIALSGKINSKNYGPNKEKRYFSVSTSYIEYLTSKSVENKS
                     NYNELKYTPKPITDDDEIPFKF"
     gene            27895..29997
                     /locus_tag="ACGI68_000523"
     CDS             27895..29997
                     /locus_tag="ACGI68_000523"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFH0136981.1"
                     /translation="MKSVIIAEKPSLARTIVDAIKEDFSKKNGYYENNKYIVTYAFGH
                     LFELLDIEDYLNREKTYWNINDIPFVPKEFKFKLKSDDGVKKQFNTIKELVNRSDVNE
                     VISCGDADREGEVIIRLILNEIKVDKRVKRLWLPAQTEDDILKALSDLKDINKYDNLY
                     LEGLLRTRVDWLYGINLTRLLTLKKGKNLPVGRVIIPIVDVIYRRDLEIEKFVSKDYF
                     QPELIILKNGEEIRCNINELKFDSKRDCDSEVIKQKECNLVVENIEIKEVKKSPKKLF
                     SLDKLQNKLSKDLKLSADESLKIIQKLYEKGYVTYPRTNTEYLAESEKERIKKLINIH
                     DSGSNILEMKDKKSIFDDTKIESHSAIIPTEKLLKKEDLDSIETSVYNIIKNRFICNF
                     LKEQTVLEETVTTLKFGKYEIKFKGTVIKKLGYLAYENDLKEKAVTKFSKGEKLDFNI
                     ETVRKKTQPPRHLSLEDLNNYLKNPFRNNEMTEDEEYNLMLKGVEIGTVATRAEIINK
                     AKRLQYIEEKQGIFFIKELGKILIKSLEDLNINMDKNKTVELSKILKQVYRREIESDK
                     AIKIYSDDLVNTVNNSKAIEVKKIDSNVKEKEIIGICPKCRKNIFEGPKNFYCEGYKD
                     TNKCTFYISKEDKFFQNRGKKLTKSMVKNLLKDGHVKVKGFKKKDGSGSYDAIVNMDI
                     KEKWVNFSLQFENKKIKR"
     gene            30021..30914
                     /locus_tag="ACGI68_000524"
     CDS             30021..30914
                     /locus_tag="ACGI68_000524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136982.1"
                     /translation="MDKIKKKIDKIIKSSIKIFNFLEKKIQRINENRTISNKSITYYA
                     LTIAIVFGFGFFFNSNALLNKEKDMRSTPLYEKQVIGGMSIEIRDRKYNPATSQVQFL
                     IYVDNDNNFETKKMSFELREQNNPAELIDLKARQIDANNYVIISNVPRKWTVLSLGVG
                     EKVVSGDKYTIMNKIKIYSDIRGTEKSYGLKEKNTNEYLGDAIDKEIIVVQSKIKDIE
                     KDYLSEENEIKRIENDIKSLEESRKYQTESELENSNSEINSLKNSIIICKDKMNDYDK
                     QINELKQKVKKLEQKKVDFLN"
     gene            31110..31421
                     /locus_tag="ACGI68_000525"
     CDS             31110..31421
                     /locus_tag="ACGI68_000525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136983.1"
                     /translation="MKPLKSLKIRDVPEETIIKLDEISRKQNLSREEFLRRNLKTIAV
                     ADEIYEVESKYKLLIDKVLGILNLNTIVLKKFMDENLITFEDIDKNGEQLLKEMSEID
                     E"
     gene            31414..31818
                     /locus_tag="ACGI68_000526"
     CDS             31414..31818
                     /locus_tag="ACGI68_000526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136984.1"
                     /translation="MSKVDKHFLLEKENVEYIKKLKEEKFHTESLAINHIIKEHREKE
                     TNDTQMIISIMIEEVFNRLNPELKKYNTKLNATDKNVQIILELINGILVENNLDDIAM
                     TDELESSSLLKARKTVTKKIEKSKYSKGKALY"
     gene            31818..32960
                     /gene="mobP2"
                     /locus_tag="ACGI68_000527"
     CDS             31818..32960
                     /gene="mobP2"
                     /locus_tag="ACGI68_000527"
                     /inference="COORDINATES: protein motif:HMM:NF037847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFH0136985.1"
                     /translation="MTSPGVVHISRFVTSTNNAFKNYIDYLDRKEATRNDAFNRYSLY
                     NDYMGNPIKTTGLFTKEKDVLLDIEKEKIKELFVMAQQKGSIMWQDVFSFDNKWLEKQ
                     GLYHSGSKSLDEEKLKHAVRESMNELFEKDNLKGSGIWSAAIHYNTDNIHIHVATCEP
                     NPTKKRGKRKPKIMYNMKSKFVNSLFDAEKDYEKINEIIRENLIKSKQKFSSTSDYEM
                     KKMFKKVITNLPADKRQWHYNYNTMKNSRVYLDKMTAYYINTYKKKEYNELLKKLDKQ
                     EEVLKTIYGVGKFEKYKDYKANKIKDLQTRMGNTILKEVKVFIKEKENRADYCKSSTR
                     NKTMNIHISRRDIDRLSKALSDESSHWKNMNYYEKLEKEIEYNNQR"
     gene            32982..33185
                     /locus_tag="ACGI68_000528"
     CDS             32982..33185
                     /locus_tag="ACGI68_000528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136986.1"
                     /translation="MDFIMSNNNKGIVTNVHGDVLTIEDIESNDVTEIIMKRGFVEES
                     EIEIGCLVEYDKYKKIVIQEDIN"
     gene            33236..33874
                     /locus_tag="ACGI68_000529"
     CDS             33236..33874
                     /locus_tag="ACGI68_000529"
                     /inference="COORDINATES: protein motif:HMM:NF019357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136987.1"
                     /translation="MLLKKHIFKVKLVMLSLFFLFICTVKIEAYRDNEIDNLKPHYNQ
                     IEELEYNYESMIEVDSDNNEKVLKLYKYMSLIPNNVINEFAETGGKIVLTHESLQDRF
                     ISEYALAGVYFDKTIYLSDKENWSEQSLIHEMGHFISDLYNDTSDSIFLDIYANEKNY
                     LKLNSDKDIDYYIKNPDEYFAQCFEEFIINPDRLLKNNLRTYKYIRFCINRV"
     gene            34232..35611
                     /locus_tag="ACGI68_000530"
     CDS             34232..35611
                     /locus_tag="ACGI68_000530"
                     /inference="COORDINATES: protein motif:HMM:NF019997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFH0136988.1"
                     /translation="MKKYNEKDIEKLKAKIDGHLKLVDKKLELHLNTKEEVENYLKFM
                     AKFYDYSNGNRILIENQFRGAEAVGSFKFWKEKGFAVKRGEKGAKILVPCFGKYLMYE
                     NGETKNLKYASKEELEKVKRGEIKTKKKLCYYMEGNVFDISQTTAKASDLPKIFPNRW
                     LEGDISNYSLMYKAMENIAEKIGAKIVEPYRELGVAKGVTYGNKEIALNPRNSELQNV
                     KTLLHELAHAKLHGGKKHENYTTEEREFQAEMVAYTTCTYLGIDTSKYSLSYIKHWTK
                     DTDIRHKKDLLKEVSDTVKEYIEILELTLEKERALTKANNKDKELDNNKEKESNIKDE
                     GLKLENTYVKFTSSTAKEIEKGDSWEFNIANELIEMMEKKCSIDESKHEIKIKIYKNE
                     KCTQLLSSELKFNIGDGKAKDLDERISAAVKNGEMNIKESSLKENILNKFNSTLKDRQ
                     SKERLDRCM"
     gene            35650..35850
                     /locus_tag="ACGI68_000531"
     CDS             35650..35850
                     /locus_tag="ACGI68_000531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136989.1"
                     /translation="MKIKFSDENLKQKMLLQELQNSYEVRKLNHITINKTNSKEEKGD
                     LKSRFFNGKIDKNEKKIIDCFV"
     gene            35971..45981
                     /locus_tag="ACGI68_000532"
     CDS             35971..45981
                     /locus_tag="ACGI68_000532"
                     /inference="COORDINATES: protein motif:HMM:NF027718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HFH0136990.1"
                     /translation="MYNKKIISILLSTVIASSTSLGITPSNINVFANKTTYKNNLQSE
                     NENISLHMGEYKDDNGILSFQGAEGKLKEATYLRFDFNRNAYNDVTVLYTLNSQEESI
                     TGKNIKIPIKNQTELDEVLKSLKINTNGNKKLGFNVLLGGLPIFKSVEGKPSRLYKPE
                     RAIDGNNDTFYGAINQNDSTFTILFEEKTYIENVLIKANKNFTIEGLKDNVWKTIGDS
                     TTDSIEINGKKKINVEKDIYDGIIIKQKHSGVDSCTINEIEFNNGSVEEKINYNSNVC
                     SLDLGNKVVNEDGSISFKDFNAKGNEVKLLISFEKNSDKNKISYMQNKQAVEKKGDCE
                     IDVNNETELNEILESIKIYYDNKTENSASISLITNNNNSIENVPKGIIAEGGASDSVH
                     TAENVFSGSNSFFISNSSNTLTLKNTKDIYLNGVAFLNGNSQTIIKGLKNGKWSDIGT
                     HSSNDFEMINLNSGYYDEVKFTSSSTLDIRNLYLRYSDIKENIKLDIKEEVADKIDLG
                     KIEYSNGKITFKGATGRIQDKANIGISSKTNVEINYILNGEEITKDGKEIIVEDLTQE
                     TLDEFLSNFSISYNEYEGCELDISLLGNSSTIIQTQNYTIPQIQGDLELKLGNYENDG
                     NGNVVFPNAKGLLYRKGNLNINFETGFSDDIEIKYKLKNIEYTKKENNLSIQISEQAE
                     LDEVLKTLKIRCDSNLEYNLNVTLAEERYIPLGTTSDKPEALTGGWYSGRSSTATINF
                     KSPLKMSTVSLTKTSGVDNCFYIKGLKNGIWKEIGRFTTVYPNYDYLKTREIDVQRDS
                     YDAISITYTVANGWGSSNVSKLGFLDKVSSDIKIKPDINLKVTLEDNLEKANLKWNSD
                     LEAYAYNIYVKKKEDANWYLCKSVTSNINETDDYNINDLAAPSIPKYEIQLDGLKKGI
                     QLESDDLGTEYSYYVNAIDKNGNKIGTSNVENVNVQSDFKEFIYEVNNNATTPIELKN
                     KSNGFIPIGELIDKNYLHVASVDNIGNISDIKTIPILGLFVDGPPIINVPGDCSLKAN
                     SKIDINEGVSAIDEIDGDLTSKINTKIISPKGDVVYDIDTNILGNWIIEYKVTDSKGQ
                     TTEHIRKINVIKNINLKLNIGKLLTTSKFTQFPNAKIEFKEDEKVNGIKMYFSNEFND
                     SMKVHYTLNGIEKITTDRSIVIDDELTIKDAENLIKSIKIEHDNSKKFNFNIVLSSND
                     AESIIYNYENNHYYKFIYSNGISLEDAKKEASKQEFLGSKGYLVKIDSESEKEVLSML
                     TNQSFWNEASTDNKEKNGFVVEFGLEDKLPSPDTINIFDYESIINIEPFESKQDIVLN
                     TLVDKKENKVILDWNDVEDVDYYKVYQKKNGMGDFEAMSSNNYAKGKKVKVLNIYPTS
                     SVKDTFKNWVTESGQELIDVDTVSNGDFNNNPDFYLKDESGEYKYDVVACGFANITTT
                     TSLPEKSVLAINDAKKNNVGVLLGHHYIGESIGNFPKYSEEYFMNKYKNTDEVCSHEV
                     ELDKSHEALNYPNELGKVGDVLKIPHTHSNYNAFPFEDDIVIKFHNPKRHAYPYNCNK
                     SYEGYSGEINGKNVSQNAYLSIHENTAQIQTGHSGTNITTDEKNMLANTVFYLAQKLD
                     ESYLEDINGKDTNEPTAPTHKLYQIGIKQGLKFDSEDTGSKIEYYVEGVSKYGDVTHT
                     SNKKMVEIKSGLEGFSYEVSNNPTPSSSLGDNINSTDGFISAKELASKRYVHIISMDK
                     AGNKSQIVSLPITGLFIDNPPVLDVGGNISVSQNEEYDVMKNISAEDDYDGDLTKDIK
                     VSIKNPNGEIANKFDTSVLGKWTIEYSVKDSKEQESREEKTITVKENLDYLNVNLGEP
                     NKTRFCTEFPNASIINNSDKKINGIKLYITSGYNESIVINYTDKGIKKSFKGQQIILD
                     DSYTTEEANSLVKSLKIEHDNTTDFDFNIRLGNSLAEDIVYNSQNKHYYKFVNASGIT
                     WEDARKEAEKQELEGVKGHLLTIESSEEQSIINSMSPQSIWINLKRLDGKWVSVIEDE
                     TKEATYFNWIDGEPNNQNGIGVQEDYVQSIKENNGKWNDIPNDTKLPGLLPKFKINGY
                     VVEFDVNKEISNLNTYEDTAHIKKMISNQDIVLNTVNDDENNYVKLNWNKYKNAEYYK
                     VFRRELNSDSDEFETILENTKENCINDMDGKDITPPIYDFSLIFDKEMNSKLEFHAEE
                     VGTTYEYKIESYDKDNYQTAYSNTSKSTVTSKFKGYSYVVDEKPDTELDNTINNINGK
                     FSFENVTYNTYLHIKAIDEQGNATKTKHIKLADETHNNEPRITVKGKNRVIKVGEKFD
                     KLDGVKAYDFEDGDLTDRLVVEGEINNQVKGVYEVKYSVEDSKRLRSEVIVKVHVTGD
                     MNLNIEDQEKDELLISWNKVKNAVKYKLYRTDVNGYDFEEKGVFTDTTFIDKSANDVS
                     SPVITYLNEVIIDEETSKIGRLTILSKNIIKLADKEINKDSEQNTELKLLARDLASTY
                     RYFVEAIDINGNVVNSSEIKNGSVSVGLAGFNYIIDDKEDTEADNFVNAKNVLDIDIS
                     NSKFKFLHVRAIDLNGNVSQTAHHLISGKYDTNHIPVIRGLNTIRVKEGDKFDQLKGV
                     SAYDTEDGDLTSKIEVEGKVDSNVNGKYILTYKVEDSQGNKREVKRNIIVYADMEIKV
                     TPDFEENNLVISWEENPLKDKVFYEVRKYDYEIGNYKTIGYTEDLSFVDKNAKDIYGS
                     KINNIHGSDMDTDELEDKIILDIDAKDIGASEKYQVRTRLKEDNLILRDMTNEEGVEW
                     GVGGNLSGVVKSFKWKLSNKREDNLESGMITTSISPNKVEFEHKGYKYIHIMPIDSYD
                     NQGDIIHYEIGKPPVEEVIEPPIIEINGVPVINGVSTATIKIGDEFKIMEGVTAKDYR
                     GQDLTNFIKVKGFVNRYEKGEYNLTYSVIDFKGQETSRTCKVIVIDYSEQLPDSKPDK
                     INSSPEVNFWNEDYIYVGEKYGEDRILNGIRVIDEEDGDITYKVTYETDLNINEVGTY
                     AILYSVSDDDGNKVKFTRVVYVIPKGEYKPDLGNNENWKNGRWARIKTYNKYLNVGDT
                     FNIKSGVYAYDDVDGDITSQIKASGIVNTIKEGAYNVTYKVTNSDGNTTVAGATIFVK
                     DGGWDNNNGEKPNPNPDWGNNDNDDSHNNHIPNRPNQPEQDEDDINGDKLPGVDDNTD
                     KDKNDNIFIDKDGNKTYPDGTIEKPNGTEVKPNEEGEKPTIDDDGNIIVPPDGVIVKP
                     DGTVVIPPNGAIIKPDGTIVMNEEDKNKEENKQEEENKHTNKENEVDKHNVINIKNEK
                     DKKEEQKSGQNINPETGDKGIIASIAIFTTSLVGYLFLNRKKNK"
     gene            complement(46039..46419)
                     /locus_tag="ACGI68_000533"
     CDS             complement(46039..46419)
                     /locus_tag="ACGI68_000533"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFH0136991.1"
                     /translation="MYISLSLDEILKYHQKIITKSGGFQGVKDIKLLDSALNRANVTF
                     SGVDLYKTIEEKISVTTHSLISNHCFIDGNKRIGISTMLLLCKLNKISIEYSQSELVK
                     LGLEVASNVADETYILDWIKKHKQ"
     gene            complement(46419..46586)
                     /locus_tag="ACGI68_000534"
     CDS             complement(46419..46586)
                     /locus_tag="ACGI68_000534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136992.1"
                     /translation="MATAKNIINEFYNLDENEKEEVIKELLNFNAKKNLDKIMDDTID
                     DNIEALRKLAE"
     gene            complement(46690..46926)
                     /locus_tag="ACGI68_000535"
     CDS             complement(46690..46926)
                     /locus_tag="ACGI68_000535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136993.1"
                     /translation="MEVYIISETFGNSSNESNIIGIASSKQELNKCIIKMKKENLINT
                     NLEINLNSNINIETLNKNIDFINIEKVELNIFNC"
     gene            complement(47328..47849)
                     /locus_tag="ACGI68_000536"
     CDS             complement(47328..47849)
                     /locus_tag="ACGI68_000536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136994.1"
                     /translation="MSTRGLIGYRTTKRRDNTNNIFGIYNHESSEFIYKGLDVLEIYY
                     DHSKKDFINMFKAKTWDNSVDAHVNDTLSIFSLKKEKFKNYSSFLLKGVFCEYAYIYN
                     LQTDELEVYETIKNKPYFKNQYKYESNYYLNLAFIVTRENVHFILNLFYYSLKLGDDA
                     LIRMYMRDLKNKK"
     gene            complement(48173..48505)
                     /locus_tag="ACGI68_000537"
     CDS             complement(48173..48505)
                     /locus_tag="ACGI68_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0136995.1"
                     /translation="MHIQKIPKSEFKVMKFIWEINDIITSKMVTENMREKYSWKTTTT
                     LTFLKKLVEKHFLNAEKINGLTHYRILITKEEYIKFATKKFLEDIHDNSIESLIDSLL
                     VILKTPKK"
     gene            complement(48544..49077)
                     /locus_tag="ACGI68_000538"
     CDS             complement(48544..49077)
                     /locus_tag="ACGI68_000538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0136996.1"
                     /translation="MIKIESKNGKKVIKKTIEVEYIDCNINKNGEWIKENVSAYKLKS
                     LSNDDNIDFLYYLDEKGDKFEIFIEKYFTLSSRNSTLYIYAFCPDLFIRKDLLERINK
                     SNIKISPTLVCFESPRKIIKIKKFLFPRYWTNYMKVTWLKNNNHLYLVNTKFTFTKNV
                     VKCKQYINKHQDYIYNY"
     gene            complement(49199..49549)
                     /locus_tag="ACGI68_000539"
     CDS             complement(49199..49549)
                     /locus_tag="ACGI68_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0136997.1"
                     /translation="MNLDKIYIIIGNRIKNFRKKLGYSQKTLAKKVGVAYTYIGQIER
                     AEKKASFHSLLKIANALEMPLEILLENIILNDDEVETVSSECYELIDALSLKEQKIML
                     KLIKEIIEYGEVSK"
     gene            complement(49743..50609)
                     /locus_tag="ACGI68_000540"
     CDS             complement(49743..50609)
                     /locus_tag="ACGI68_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HFH0136998.1"
                     /translation="MNSIVNYKNRGNFGRSNYRGNTSGKIILDLHKVYQFDSISDYMS
                     GSFTTEDVANYLNISSNCYDLNNGFDLIDNDIKERNEFIFWHPPYWDIVKFSGNVYGN
                     EILKNDLSHIKDYYEFVKKLNFCLTKQFNTLEKGGRIAILMADVKKNKKLYSMLLDIN
                     KLGTTEQIIIKQQNNCMSDNKIYSNNNFIRIAHEYILILRKDNPLIVPFSITKTDFFD
                     IRDSLKITWKDLVANVLENLNKKAYLNEIYNKIEGHKKCNSNKNWKEKIRQTLQENKI
                     FYKIDRGLWGLK"
     gene            complement(50708..51148)
                     /locus_tag="ACGI68_000541"
     CDS             complement(50708..51148)
                     /locus_tag="ACGI68_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFH0136999.1"
                     /translation="MKKIEIVKVKLVKEKDLFVNNIHIKEPKDCYKVILNFLDNPDRE
                     HFIILGLSTNNKICIISTISIGTLNSSLVHPREVFKIAILSNCSKIIISHNHPSGNCK
                     PSQEDKNITNRLIDSGNLLGINILDHIIVSDESYFSFKENELLF"
     gene            complement(51903..52127)
                     /locus_tag="ACGI68_000542"
     CDS             complement(51903..52127)
                     /locus_tag="ACGI68_000542"
                     /inference="COORDINATES: protein motif:HMM:NF042874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6718 family protein"
                     /protein_id="HFH0137000.1"
                     /translation="MCYVIAKKFNNIGCLALRTEQGDKLGALTEYLSLATLGKNVQIV
                     VLGDIRSYREYEPYKLLDDEGDFISQVLKM"
     gene            complement(52099..52473)
                     /locus_tag="ACGI68_000543"
     CDS             complement(52099..52473)
                     /locus_tag="ACGI68_000543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137001.1"
                     /translation="MELNKCFNDLLGIRIIVDSYYELFEQDLSSFRLADMRTGKANDD
                     GYRGVHLYYQKSNFHYPIELQINTKVDRQINDLLHMYIYKHTKNNLIGKEVRKLFDEG
                     NFKNIEEFKGVIEDVLCDSEKI"
     gene            complement(52451..52666)
                     /locus_tag="ACGI68_000544"
     CDS             complement(52451..52666)
                     /locus_tag="ACGI68_000544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137002.1"
                     /translation="MKFNFYGITENILDELSYESKLGKSLKNTLRKFNKDDIFKEIRN
                     ISRYLNTRKIDFKFPVSYRIYGIKQML"
     gene            53027..53782
                     /locus_tag="ACGI68_000545"
     CDS             53027..53782
                     /locus_tag="ACGI68_000545"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0137003.1"
                     /translation="MKTICVYNIKGGVGKTTTTINISAILAEKGYKVLMVDADPQANL
                     SNTSENYNHKDYTIAELLTKKGMLTNEVIIKTEFDNIDLITSDIRFSYSEREILLDMG
                     RKPHDRLKKALEQVKDVYDYCIIDCPPAWNLITINALHVSDEVLIPIVIDRYTLDGVN
                     DLMQKINEIKEEYNPNLTIKGCFVTRDEPTTVNKQVKEGLKEFLGSILLRTSIRKNVS
                     VPESTFECKPVIFYKKNSNASKDYYSLARELNL"
     gene            53802..55667
                     /locus_tag="ACGI68_000546"
     CDS             53802..55667
                     /locus_tag="ACGI68_000546"
                     /inference="COORDINATES: protein motif:HMM:NF018926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFH0137004.1"
                     /translation="MAKNFMKNNAVTKLENIKNEVQKSELKEQEQFRIEYIHIDDIVP
                     SKKNFYSIDDESISELADSILEVGLLHNLLVRPNIKVFEMILRVYIADLEAYENGELI
                     GKWLRLPEDEEKIEKILKGMGNKYFIKDYITNIEGLDIEGKGINELNELAKNNQHKKE
                     FEMMLKIEVSNLDTYENANECTSKWIDLPSKEETIKEALEYVGDRYFISDYVTNLNIK
                     GLEDKKISELNELAKNLIDGLEYEKYELLSGERRYRAHKRLVELGYKKFQLIPCQVMS
                     LDDVDAEIALISANAHNRPLTDYEKLKETQKLIDLHKLKKANGEKVTGIHNAVSEKLK
                     LKPTQTKRYVTMAKNLIPELEKMLELEKLSVSNASIFSALSKENQRALYQSLKDKDDM
                     LEITKKEAEDLKNRYKDLEEEKNIIEKSYIDKIEQLTKEKELKEKELKEATNGINEKI
                     EMIKKEMETKIKVKTDGEIGEYLEEIEQLEKEKKELEIRHKEIKEEQEIKLEKIKEEQ
                     QELVLKAREEVKLKAEHILQDFEEDKKRLKEENEKIKLENEKLKKVLDSKDFDDREKK
                     AIYETKITLTKLKSDVIRVTSLMKNIPSDEEILKDIESLQRLFKTLESTIEIHAK"
     gene            55942..56526
                     /locus_tag="ACGI68_000547"
     CDS             55942..56526
                     /locus_tag="ACGI68_000547"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137005.1"
                     /translation="MDIIKQINYKKNELGMNQIQFAVYLDVSLNTVIKIEKGEMPSQR
                     ILNKIMKKLNIENYKYANEKKRTIYFLKSVGRLNIPSNWTECIGVTEENPGVILYLIE
                     NESNENEIWILNDNMIEFDDIKKSAEIELESIEKLMLFNSNGKGAIIPKLSVPLDWLE
                     NMNVKIESPCVSIQLYKDENIYKSKIVIKKDKIN"
     gene            56538..56747
                     /locus_tag="ACGI68_000548"
     CDS             56538..56747
                     /locus_tag="ACGI68_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="HFH0137006.1"
                     /translation="MKDYSIKNTTKKERKEIAAGALGLCMIDSKEPSKEMKEIVNKYV
                     EGEKEISEIQKEVLEMYKKRYKRGN"
     gene            56749..57639
                     /locus_tag="ACGI68_000549"
     CDS             56749..57639
                     /locus_tag="ACGI68_000549"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HFH0137007.1"
                     /translation="MEDIYLYENTSVLKNKLDIRDKKQLEDFENSITYIKLLNVDEVM
                     QNREFNFDYIKDVHKHIFEDVYDWAGEPRKINIEKSEKVLNGMSVEYCDYTQVEKEAN
                     KAIEELKNIEWKELDLDERSELFSKKISNLWKVHAFREGNTRTIMTFSVQFAKEQGFH
                     MDSEIFRVYSEYVRDSLVLASIGEYSEYEHLTSIVKDSMEIGERKFLRSNTYVKFNSS
                     FNEINENDVYKFEDAEELISVLSEKYNCSEEIKISIFLDKACKKEFFNGCIDLANCKE
                     KLSYIIKKDKPVVKREELER"
     gene            57677..58414
                     /locus_tag="ACGI68_000550"
     CDS             57677..58414
                     /locus_tag="ACGI68_000550"
                     /inference="COORDINATES: protein motif:HMM:NF015461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HFH0137008.1"
                     /translation="MEKHLLKSVLNRMTYGDIINFKMHDKDEYKEFISIEDTRAWGKS
                     NYESWASKYREIMKINKCAIEGNIFTSLIEDYCGHAYREINKYLRNRYKTNEKEVEEV
                     EEEAKKYMSKELSFILAMVLSTAPRISENIVTYRLVNDYFIQELISENKKGLPVIERG
                     FLSTSLLTDIIGVKEAYHEYDNLLKIYVDRGTIGLYVNEIFEIPERRRKEEEILFYPN
                     GYLYLMKYPYKDSVLNKKVYECYLYYY"
     gene            58664..59302
                     /locus_tag="ACGI68_000551"
     CDS             58664..59302
                     /locus_tag="ACGI68_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0137009.1"
                     /translation="MIIGYERVSTKKQNNSRQERVLEEEGIKFNKIYRDKTTGATKDR
                     PALNEMLLNVKKGDTVYCESISRLGRNLKDLINIIDTLIAKGVRVKILKEGIDTGNNT
                     YKLLLGIFGAVAEMERETIQERTQQKIDQLKEDKKKGEINTKTGKWFGRQKITKEDLE
                     NKKEFVKYYSKIKDKSMNKIEVAKILGIGRTTLYEWILIYEGGQVKRKRKKS"
     gene            59347..60153
                     /locus_tag="ACGI68_000552"
     CDS             59347..60153
                     /locus_tag="ACGI68_000552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137010.1"
                     /translation="MSFDKKRLICAGDFLIELDEAIADIIIELNKKGYKTLNCCSGHI
                     NNDGETNCYISFYESYNFTLPKNYCYESDNLIRCNFYGNKLELKKQLNQNIEILRKWV
                     NGLQTYNQVIGEIRSRNENKKHSYIKCLNKDVRIIKSEISEIVLMEIDKLDINSLKVI
                     LKEIGIKNYSKLKKNELLIILNENLDILSNEDRKEVLYWIYENNKIKFGMFQKEVIEY
                     LNISKGEFNKVSNELKDFEIGIEKLRIGGKMQSYIKYDRMKIYNIFYKAL"
     gene            complement(60188..60352)
                     /locus_tag="ACGI68_000553"
     CDS             complement(60188..60352)
                     /locus_tag="ACGI68_000553"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137011.1"
                     /translation="MKKSIKNHKKKRGNGMNKKKSNKKNTDYRKKEYNLFKLKLILST
                     LFDLIDYFKK"
BASE COUNT        25657 a         5654 c         9655 g        19435 t
ORIGIN      
        1 atagaagcag tgagaagatt atatttaaaa ggatataatg caaaagaaat cgcagataca
       61 ttaaataaag aagcaaatac agttaattta tgcatctata gaaattgtgt agatttaaaa
      121 aagatacatg aagaaaatcg aataataaga aaagatactt tgaaattatt agacaggcat
      181 aataagactt atataaatga tggttcactt cttaaatata atagacagag ttataaaaat
      241 ggtaaaaatg gtgatataaa atttgatgat aaaaggggtt caaaaccata tgacatacct
      301 ggaatatata aaaaaaatat attttagtat ctaattaaaa aatccccagg ggacttagaa
      361 tgtgagggta atactttatg taggggcgat tattttatca ctgtcaacga aatatttttt
      421 agaaccaaat gtaaataaat atctttatgc aaagatgctt atttgttgta ttaaaattta
      481 aagaggctat gtataagtaa ttctatgaaa ctgaacctat atatggaatt tgatttatta
      541 aatagatttt atatataggt tcagtttcca ataaagattt taggagacag atatttgtct
      601 ccttccccct ttttttcaca gataataaga atttaaaaaa atgtccccag gggacttaaa
      661 atttgttaaa tatattttgt taggagtatt tatatgagtg atacaagttt attacatagt
      721 actttatgga tgattataac ttttgttatt ggttttacat gtgttgatac tatagctagg
      781 tcttggcatg gaatgtcaat tatacatgat attataataa ttattattac aataataatt
      841 gcttttataa ttgagattta cacttttgat gaacctaagt ttaatccaga ttttgattta
      901 gagaataaaa tagttgaaaa tattgaattg aatggaagta ttgatttaaa taaattagct
      961 gattttgaat ttagcaaaat gtatatttgt tcgccagaag caccaatgga acatattttg
     1021 gaagatggtg tatttacaat ttctcataga ccatttaata atgacaataa ttatttaata
     1081 tttgtgaatg aagaaaatga agttgtaaaa tttacaggac taaataaaaa atataaaata
     1141 ttaaattcaa cttttaaaga atatgcaagg gaagatgcta tatttaaatt caacaaatta
     1201 gaatataaag ataaaacaat atatgagtta aactacttta gttaaaattt ctacttaatt
     1261 ttatactaat tagtgaaagg tgagatttaa atgattttta aggtaatatt aattggtttg
     1321 ctatgttttt taagtattta taatgcactc tgttgtcaat atcgtttttt aaaaaaaata
     1381 ccctattgga taataatctc tatatttgta atgattatag taaattcgat ttatattaat
     1441 aataaaactg aatataatca agattttaat ttagaaaata aaatagttga aaatatagaa
     1501 gcagatgcaa ctgttgattt aaataaatta gtcaacttta actttgagaa agtttatgtt
     1561 tgtaaaaata gtataagaat gatagatatt ttaaaagata aatcatttgc tatttcccat
     1621 aaaaaaatag atggtgatag taattactta atatttgtaa ataaaaataa taaagttgtg
     1681 agatttacag ggctgaataa aaaatacaaa atattaaatt caacttttaa aatttattca
     1741 aaaaaagatg ctatatttaa atttgataaa ttgagatata aagataaaac agtatatgaa
     1801 ttaaagtgaa gaaatgaata tctttacaaa ttaaactagt ttttttattt agtagggaga
     1861 aggataaaat gaaaaataaa aatataaatt tacaaaaaaa caacacaaag gaagaagaat
     1921 ctgttttaag aaagatatat atgaacttaa aaaaaccatt taaatttatc tttaataaaa
     1981 ttagagctat tattgaagat tttttagata accctatagt tagaatttca tttttaattg
     2041 tagctttgat tttaatgatt tgtttcttaa agtttagttg gtctgaatta gagaataaat
     2101 atgagaacag ataccaaagt aattttcaat tagactttaa tactgaaaat gaaatagtcg
     2161 agaatattaa agacaataaa tctgtagatt taaaaaaaat aatagatata gattttgata
     2221 aagtatatat atgtagttct tattcaatag taaaagaaaa agtaaaagat atatcattta
     2281 tatcttatat agaacaaaaa tctgatgaag agactaaaga gtatagttcc tttaatatac
     2341 tttcctttaa tatatttaat aaagataaaa aggatgtaga tagtaaatct ccctataatg
     2401 ataaagatta taactactta gtattcatga atgaaaataa taaagtaatt aaatttgttc
     2461 cattagacaa agattatgaa atattagata caaattttaa agaatataac aataaaaata
     2521 caatatttga atttgaagta aaagaaactg gtgaaaaaac agtttacaaa ttgaagtagt
     2581 tttttattta ataaatctaa aaaaggagtc ctctgaggac ttctttttta attgttttaa
     2641 agagtataaa aattaagtta aaaggagttg aaaataatgc ttgttgttgt acctttatct
     2701 ttattaatat taatttttct tttagttatt aatacgattt ctgaatcaag tttagaagat
     2761 aatacttgta tgagtgaaga agaatgttag taaaaaagat gtcccctggg gactttttta
     2821 ttttaataac aaaagtactt tctaggggta cttttttatt atatataagt catatgtgaa
     2881 aggaggaaga aattataata tttattaagg aggaatagta atgcaaatat caaataaaga
     2941 aacacattta ctaaagatac aagaaaaagt aaaaaacaaa tctattttgt tcttagaaaa
     3001 aaacaagaga attttattta atgaaaaagg agtagatgaa catgtttcaa aagcaggata
     3061 tggagcaggt gcagtagtat taataatttt agtagtagct ttttttagtg attcatttca
     3121 aaatacagta ttaccagcat tacaaaacaa aattatgagt atatttaata tctaatgttt
     3181 atcaaaaaaa ttattgattt ttttatatct tttatatatt atcctagaag taatgaaaag
     3241 gtaaaattaa ataaaaaaaa tatagtagtg ttgtttgtaa ttgaaatact attatattat
     3301 ttcttaaaat taagatttga tgaaaataca ccaataatca aaggtttact ttactttcaa
     3361 gtcttaatat atacaagcta tatagattat aaagtttact taatacctaa taaaattcac
     3421 attattatta tttttatatc aataatagat ataaacctac tatcatcaat caaaggttta
     3481 tttttatttc ctcttccata ttatttgatt gcttttatat ttggaggtat aggaggagga
     3541 gatataaaat tattggcttc tagtggattt ttaataggat acaaaggtta ctatacttta
     3601 ttaccatttt ttattagttt tattttagag atagtaataa attataacaa actcagaaat
     3661 tgcaaaaact taaagatagc attagcaccc tacttatctg tagggtgctt tttaacatat
     3721 tttatttagg aggaagttaa atggatttta aaagtaagtt acaaaataga aaagtattag
     3781 cagtaataac tttaattttg gctacgatta tagggtttgg ttcacctcta tatgaaagaa
     3841 tgaaatcgaa atcaacttat gtgagagttg ttaataagat tgaaaaggga aataaaatat
     3901 ctagcaatca aattagagaa tatactacaa ctgattcaaa aagtttacct aattcaatta
     3961 taaaaaataa agatgatgta gttggcaaat atgcagttgt agatatgtat aaagaagata
     4021 atgttttaag tagtaaaata tcacaaaatc ctgttgcaga aaacgactat ttatataatc
     4081 tcaatgggag taaacaagca atgtctataa caataaaaaa ttttgcagca ggtttatctg
     4141 gtaaattaca aacaggagat attatttctg ttatagcatc agattatgga gaaaataaaa
     4201 aaacaattaa actaccacaa ttggaatata tacaagtatt agctgtaaca aatcaaaaag
     4261 gtgcagataa aaaagatgac accattgaaa agggtaatga agatgagcag gagttaccaa
     4321 ctacaattac actattagtt aatgaaacac aagctaaaat tttagcagat ttagaagaaa
     4381 aaagtaaaat gcatgttacc ttagtatata gaggtactga taaaactgca aaaaaattta
     4441 tagaagaaca ggatagagtc ataaatttaa aaggaagcga aggtttagaa caattgaatg
     4501 attcaaataa tatggaaaca aattaggagg taattatgag taatagaatt atttctatat
     4561 ggggtgatcc atttagtgga aagacaactt ttagtgtaaa attagcaaaa gagttatcta
     4621 ctaaaaaaaa gaatgttatt attttattcc ctgatttgtt ttgtccaact atgaatatta
     4681 ttttaccagg acatgctgaa aaaaaatcgt taggaaatat tttatcttca acaataattg
     4741 atgaagatat tatacttaaa aatagtattt atttggaaaa aaacgactat ataagtttgt
     4801 taggatatag ggaaagagaa aatataacaa catatccaga atatagagaa aataaagtaa
     4861 aagatttaat aagaactctg ttgcaaataa cggactatat aattatagat tgttctagtc
     4921 attttgtagt tgacttaata tcctcgatct cactagaaat atctgatata gtaataagat
     4981 taatgactcc tacactaaaa tcaatttcgt attttgagtc taatacacca ttactaatag
     5041 ataaaagatt taattttgaa aatcatgtaa aggttttatc caatttcaaa tctgatgatt
     5101 ctatcgaaat agtaaaagat agatatgaca taaagtatga actaccatat aatgatgaac
     5161 ttagaactca aatgatgagt ggagaattat tctataattt gaagagtaaa aagagttacc
     5221 tatataatga tacagttaga aaattaacat ctgtaatttt aaatgaaaat aaggaggtaa
     5281 aaggttttaa actttggagg aagtaataaa tgggaagaaa tctactgaat aataagtttt
     5341 tcaatgataa aaaaaataat aaggattttt attcaatact tgatgaagta caagagcata
     5401 tgtctagaac atatacaaaa gctttagatg atagagaaga aatgattcct tatataagaa
     5461 aatatataaa tgacaattcc ttatatacag aagaaatatc agaagatgaa ttaatagata
     5521 aattaatttc tgaaatggca ggttttggat gctatgataa atacctatat aagaaaatca
     5581 taaataataa gtatattgtt agggatgatg tagaagaaat taattcaaat cactggaaat
     5641 caataattgt aaaatataaa aatggtaaga aagagcatgt aaagaattgc tttaaatcac
     5701 cagaacatgc tgaaaatata gtaaaaagaa tgcttagaaa gtgttcagat attgtatttg
     5761 ataatgctaa tcctattgta agaggacatc tagatggaaa tataagaatc actgccgttg
     5821 gtatgggtat aactgataaa gataaagggg ttgcagtatc aattcgtatt gttaatccag
     5881 aacatatgag tgaaaaagaa tatattgaaa aaggcactgc atcagaagaa atgttttatg
     5941 caataaaaac attttataaa tatggaatga gtatatgtat agctggacct actggaagtg
     6001 gaaaaacaac aacagcatca ttaatagtag aaaatacaat agaggataat aatagactta
     6061 ttacaataga ggaagatgtt agagaatttg attttgtaaa atatgacaga gacgctatac
     6121 ctaaaaatga agtaatacat ttagtaacaa aacatagtca tgactctaat aaagatatag
     6181 atcaagataa gttaattgaa ttatcactca caatgaaccc agattatctg atagtttctg
     6241 aaatgaaagg gaaggaagcc gattctgcca aaaaagctgc caatataggt attccaattt
     6301 tatcaactac acattcaaat tcagctacag acacttatcc tagattagtt gatttatgca
     6361 aagaaaaatc aaaaatggat tatgacactt tattaagagg agtaataaaa gcatttccaa
     6421 ttgttatttt tcagaaaaaa ttaaaagata gtttaaactc aaaaagagtt atggaaataa
     6481 aagagactac tatagatttt gaaggtaata ttagagaaaa tattcttttt gagtttaatg
     6541 aaatagaaga tttggttata gatggagaaa gatatataaa tggggagttt aaaaagctaa
     6601 ataatatatc agagcagtca caaaatatat taaagttgaa taatattccc aaaaaagaat
     6661 tagatagact tttaaattgg aaagatggtg ataaatatga ttaaaaatat aattattttt
     6721 atgtttgttg tgatagctat gcttgaacta tttgatatat ctagagaaga tattaagaaa
     6781 agaatcttgt atttattcaa tctacaaaaa aaagaatata ctataaaaga taaaattaaa
     6841 atggctaatt tagagaagaa agaaaacaca attgttttat tgataaaaga gactagaaaa
     6901 atattaaaat ctactggaag aacaaccatg ttttctacta tttgtttgac atcagttttt
     6961 ttatctgtag tgggaatttt tttatcttta ctaataggta atatatttct agttccgatt
     7021 ttagcaattg gtttttttat gattccattt caatacataa aaatatcaga agggcactat
     7081 aagagtcaaa tcagccataa tcttgaaagt gctttattta gtattacaaa cacatatatg
     7141 agaactagta atataatagc agcagtacaa gataatatag aagagtttga accatcaatt
     7201 aggaaagaat ttgaattttt tctaattggg gttgggctag aaccagatgt aaaaaaagta
     7261 ttagtaaaat tgaaagatag aattgataat gatattttta aagaatggat aaagagagta
     7321 attgattgcc aaaatgataa tgtattaaaa tcgcccttaa aagggattgt aaaaaaattt
     7381 tcaaaggtaa aagttcttaa taaaaaatgt gagaatgaac ttattgaacc aattagaaat
     7441 ttctatacat tagttattgg atgttattca ctatttggga ttttttactt tttaaaaaga
     7501 gatttatttg atatattaat gttcactaat cttggtagaa tttgtgttgc gattgcagta
     7561 ttaatgacat ttatttgtat acatggtgtt ttaagactga aaaaacctgt agattataaa
     7621 atgttgaaat aataaggagg tgtggatttg tcattactaa ttacaatagt attattggct
     7681 atagcgattt acttgatatt agcagagata cttaatatcc caacatatag aaataacaaa
     7741 ataatgggta aagtattaaa aatgaataaa gatggaaaat atattgataa tcttactact
     7801 catatggcta taaagttatc aaagtttatt aaaataagtg aagaaagaaa agaaaagata
     7861 agttatttat taaaagtctc agggataaga ttatcacctg agacttttat tgcaaatgct
     7921 tttgtaagtt caattataat gcttattttt tttataccca catacttaat tatgccatct
     7981 gttagtgtaa tttggataat tttagctata tatgcttttc gtgtagaaat aaaaaaacca
     8041 tatagattac ttaaagagaa aaaaagaaaa attgaatgta atctagcaga cttttcagcg
     8101 acattagcca tagaattaga acataccaga aatcttgtga gaatatttga aggatataag
     8161 gaatttgcaa atccagactt tgcagaagaa ttagagataa cactagcaga tatgaaatct
     8221 ggtagtgata aacaagcatt aaaaaggttt gatacaagaa taggtagttc atatttaaca
     8281 gaagttatta ttgggctaat aggtgtaata gatggaaatg atgaagtttc atattttagt
     8341 aacttatctg ataaattgga aaaatttgaa tttgaagaat taagtagtat tgctttgggg
     8401 caaaaatcta aagtagcaaa atatgaaata attatgttaa ccgttattgt catgattatt
     8461 cttgttgcat ttgctagttt atttttaaaa tattcaaagg aattatttta aggaggtaaa
     8521 ctaaatatga aaaatttatt aaaagatgaa agagggttta tagactctac agttggggtt
     8581 atattaatag caattgtagc atcccttttt atttcactag gatttgtcat attcccagca
     8641 cttgatacac aagttcaatt aaataatttt gctaaagaaa tagttagaga agcacaaata
     8701 actggagaaa tagataaaga tattgataaa aagataaaaa acttagaaga taaccttgtt
     8761 aaacctgata aaataatttg ggacaataaa tatataacag gaactaagaa agtacaatta
     8821 aatgaagaaa taaaggttag agttgaaaaa actattgata taggtttttt tgattttggt
     8881 cgttttccag tacctttaca tggaagccat tctggaagga gtgaagtgta ttggagaaat
     8941 tgagggatga aaagggagct gttaatatag ttattcttgg agttgtttta gttctagtat
     9001 taattattgg tctatatttt gaaaaaatta ggttacaaat agttgctttt ggtgtaagaa
     9061 atgcttttga gacatctgta actgatatct caacagaaaa ttatgatgag gtatatcctt
     9121 gtatacgaga aggatatagt gctgcataca aattagataa gtctgataag tgggagcaga
     9181 atatagataa aggaaatgta gtagaacaac ttaaaaaatc actaggatta gtagaggaag
     9241 aaggacaact tataaaatac aatggagata aaattgagtt taaattgtct gatattaaaa
     9301 taaatatatt aaatagacaa cttgctccaa gtgacaaaac aaaattagaa aaatatagag
     9361 taaatggtca acttacatta gaaattccat caaattataa tggaaaaaca agttatgcaa
     9421 aaaaagatat aaatgtaagt tcagcttaca cgtctaaata ttagttatat aacttaaaaa
     9481 aatatggaca aatatggaca aatatggaat tatatggtat actataaata tagagtgata
     9541 ataagaaggt gataaatagt gccatataaa ccatctgaat ttgcacaaaa actaggtata
     9601 tcagttaaga ctctgatgcg ttgggataaa gataaaaaac taattgcatt tagaactcca
     9661 accaatcgaa gatattatac tgaagaacaa tacttaaaat atgtaaaaga tagtggagag
     9721 aagaaaatat tgatatttga ttgtaaagat gatataaaaa gtgaaaaatt aagaaaatat
     9781 gctaaatcta tcggatgtga agaggtgaat taaacttaat ttagagagga tttttattat
     9841 gaaaaaatta aatataaata tcaaaaaaaa aacaataatt atgtgtagca tagtattaac
     9901 aatgtgtatg gtattgttat tgtctttatt attgatgaaa caaaaaaaag aaatacctgt
     9961 agctaaagaa tataaaaaag aacatgaaac tgacattaaa aaaggtgatg aaataaatgt
    10021 ccctaaaata gatacttcta aagagtctat aactataggg gagttaaata ataccaagag
    10081 caaagatgaa aagtttgaaa ataaaggtaa ggatatagat aaaaagataa ataacaattc
    10141 taaaggagaa aatggaacta aagaagaaat acaaaagaaa acaagaccaa taaaaagacc
    10201 aagtgatgca aaagatgaag tgatacataa ttctaataat caagggtctg aggatattag
    10261 tgagaatgaa gaaagtaata ataatgaaga agtattgaat ccaggtaata tacaagaaga
    10321 agttaaaatg gaaggtgaat ggggaaatat aatagcaaat atgagatagt ttaatatata
    10381 ataaagttaa gttatttatg agggggaaaa tttatgaaaa ttatgaaaag aatattatca
    10441 ttagggttga ctgtaacatt tttagttgga agtagtacat tagtttcaaa tgcaatgcaa
    10501 cttaaagata ttgataaaat agttgggaaa aacagatatg aaacagcagg tcttattgca
    10561 gataaacaga aatatactac tgctataatt gtaaattcag ataaaagtat tgctgatgga
    10621 cttagtgcaa gtggtttagc tggaagtgaa aatgcaccaa ttctattaac tcaaaaggat
    10681 aatgttccag atgaaacact tgaaagactt aacacagtaa ctaaactgta cataattggt
    10741 ggggaagatt ctattagtac agatgtagaa caaatgctaa ctatgagaag aatggaagtt
    10801 aaaagaatta gtggaaaaga tagaatagaa acaagttata atgtggcaaa agcaatagat
    10861 tctataaata aggtgaaaaa tgtaatactt actaatgcct ataaaggaga agcagattca
    10921 atgagtgttg catctgtagc agtaagagat aaaactccaa ttatacttac tgatggaaat
    10981 aatattccat ttaatacagg aggactaaag acctatgtaa ttggtggaaa atctagtatg
    11041 agtgataatt tagtaaacaa gacaaaatca gtaagaataa gtggtataga tagatttaag
    11101 acaaataaag aagtaatatc atatttctat aaagacgtga aagagtgtta tataacaaaa
    11161 gcatatacat taattgatgc attaacaggc tctacaattg caaaaaataa accaatagta
    11221 ttagctgatg aagttagtga taaaagtgtg ttaaaaggaa tgaataagtt tgatgtaatt
    11281 ggtggtgtaa acaatactac aattaataga tgtttaaatg ttttaaatgg agaagaagaa
    11341 aagaataacc cacaatcttc tgatgataat aaaacaggaa atatacaaga tgaaatagaa
    11401 atggaaggtg aatggggaaa tatagtagca gatatgtaat ttaagataaa gttttaaaga
    11461 gtgtaaattt ttacactctt ttttagtttt gatttaagga gtgaaaatat tgaaaaaaac
    11521 aataactttt ttatgtattt ttcttataat gccaattctc gtatttggag atgggaatgg
    11581 gaatgtagat actggtggag gtaatatggg aggtgttgtt aaaggatata aatggacgcc
    11641 tggtgaagaa ggtgttagag taactgttat aaaagatgaa actaaacaac cagtaacgac
    11701 acctatagat ttcacaaatg taaaaaatcc aaatgtaaaa tatcattttg gaaaagtatc
    11761 taaaatatca tataaaaatg gaactaagtt aacacgatat aatacagcta ctaaagggta
    11821 tgtaagtaaa agaccaagta gtggtaatat tcccataata attaaaagta ataatcgtaa
    11881 accagacatg aaaaaaataa aagcttattt ttgtgataag gtatttatac aaacactggt
    11941 taaattaact ggcatgaact atgaagaatt aattggtgga gaatacaaac ttttaataga
    12001 gccaattgga tattgtacaa taaaaggtga aaggtatgca cttacagcac atgaagcagc
    12061 actatatgat gaacaattaa aaggtacttt aaggtcaaaa atgccttcct ttactcataa
    12121 aaatctacct tttgctttat ttttagaaaa tgcagactta ggttttcctg cttggaaggg
    12181 aactactaac aataagcaat tgaactctac tataaaagat aaactaggct taggtataat
    12241 ttactttgga aatgaaagtg aagaagaaaa accaccagat attgatataa aaccagaccc
    12301 tacacctaca ccacctaaac caaatgaaga tgaaggaaat acagatacaa aagagtatga
    12361 atacagaact gatacagatg ttatatcatc tgtattaatt agtggtagaa atagaactcc
    12421 agatcatcct gtgaatgtaa gttttaaaat aaaagataaa acatatacaa tgaaagacat
    12481 ttacttacca aaagaaggtg gagaacaata tgcatgggta aagtggcata caccgaaaac
    12541 accagaaaga ataattatta ctgtttcatc aaatgcaaca agtgaaacgt ctaaaataat
    12601 tgctaatgta aataatctgg aagaaaaaac acctccagac ccaactgcga atgataaaaa
    12661 gccagataat tataaagtac cacctaaacc agagaatcaa acaacatcta cagaatatgg
    12721 agaatgggat tgttactggg tatcgcattg ggtatattat tctgatggag aaggaggagg
    12781 cgaatgggtt gatgaaggct ggtgggaatg gaaatggata ccatataagg ttactttaag
    12841 tgctaaaatg aatttgaaag cagattctaa tgttcctaca gcaagaaata atggaaaaga
    12901 aataaaatcg ggttatggtg ttgatactgt tgtagattct aaagtggggt ataatgctcc
    12961 cgaaaatagt ttgacaggag cacaaaatgt aattgttaca tttccagaat ttagctatga
    13021 gaaatataat agagttttac aaaatacaaa gataggttat gattcaagtt ttgtattcaa
    13081 gaataataag tattctacat ataactcaaa agtgcatttt acaccacttt ggtttttgga
    13141 tggaaagtat ataactaatt ctaaagtaat ggatgtatgg actcctacag ggatgctgag
    13201 cattaataca aatgattatg ttgatattaa aaataattta tatgatgact ggcatatagg
    13261 tcctgtaaaa taagtttcag aaagcaagaa gcaaatttaa acagtcacat taaatttaaa
    13321 aagtgtcccc tgggggcact tttttagtgt acaaatattg gagtgataca ataaatgaaa
    13381 tgtactggta aaagatattt ttatatagag aaaaaagata ttgaagataa aatagattac
    13441 attactaaat ctaaagaaaa attaaaaaaa caattcaatt taaatgatga agatatatta
    13501 aagatattag aatctttgca aataaaacta gaaaaaatta ataagaaaat ttcaaaaatg
    13561 taatactttt atgaaaaagt cccctgggga cttttagagg gagagaacac tatgataaat
    13621 aatattgaaa aagttgtttc attaggtatg attcagtttg taactatatt attgctaata
    13681 ggttactatt ttggaggatt aaaatatact ttgatactag ggttttttat aatgttttta
    13741 ttaggcataa ctattatcaa aacaagtctg aattaaaaca ttagaaagga taattatgaa
    13801 tggagataaa aatataaaat ttacaaaagt tgatgagaag aggctggaaa aaagaaaaaa
    13861 acaaaaagag atacaaaaag gaatttatct tatgggtgaa tatgaagaag gaaaagtaag
    13921 agtaagtaca aatctaccaa tttctgttgc tgatgcactt atatattata cgaaaacaga
    13981 atcggcaaaa gaaaattcta ggtctgaact tttagaaaat attatcctag attggacgaa
    14041 agaaaacata tcagaagaat tattaaaaga agggtgacca tagatgattg atatagaatc
    14101 agtgtttgta gacttagtca gtagaggaag aaaatggagt gtaggtttat atatagaatc
    14161 tgaaacatat gaaagaataa ttaatgaaga atcggataaa gaagtaaatg taagtgagtt
    14221 gctaaaagta ttaaacaata aataaaggag aaattgacat gagatatatt tctatgaata
    14281 aaaatgggaa aaaagaattt ggtaaattga tctctgttat gagtgacaag gattgcaata
    14341 aaattgaaat catgttatca gtgatggatg aattatcaag agataatact attttatact
    14401 tatcagaaaa agacatatgt gaagaaacta tttttataaa ttacgattat gaaaaactgg
    14461 agtttcaaat aagtattaaa gataattttt atggttatag tttattatcg ttctatagac
    14521 tagaagatat gttaaggtat gtaataaaat caattgataa tattgatatt attttcgtag
    14581 atggaactag ctttagtgtt aaagtcaatt ataaaaaatt aaaagatttg gcaaaagaaa
    14641 caggtgtaac aattattctt ttattagaaa aagagacgat tgaacttttg aatacattga
    14701 aatttagaaa acatataaaa gatattgatg aaagtcttat tttatatatt gatagatata
    14761 aagagatacc agaaggttat ttctataaag taagtggaga tgaatttaaa tctattcaaa
    14821 taaatacgcc tacatggata gaagatgtac taaaaataac aggtcttgga tataagtatg
    14881 taaaagtagt cgatttttaa aataaaaaag agaagaggtt aattatatga ttattaaatc
    14941 aattatattt ttattatatc tattttccat ttgtatttat ttaataggta tttattctgt
    15001 taatctaaaa ataaaaagta ataaagaaaa ctatgaattg ataaaacaat gtaaaaagca
    15061 ttatatatat atagtgagag aaatggcaat aggatttata ccaatagtga attttttaat
    15121 aggagttatg ttaataaaaa taattatgaa aatggatgat tctgaaatta aaaaagtcat
    15181 tgaaaataga aaaaatagat gaaaaaatta aataaacata aaaaagatgt ccccagggga
    15241 ctttttttat tggaaaaatt tgaagggggt gtttgcaaat gttttgtaat agtgtttatt
    15301 ttataaaata agatgtacta aaaataacag gtcttggata taagtatgta aaagtagcaa
    15361 atttttaaaa tagaaagaga aaagaggtta gattatatgt attttatatt atatattttt
    15421 ttatatatat tttctatttg tatttattca gtaggtatgt attctattaa ttcaaaaata
    15481 aaaagtaata aagaaaatta tgaattacta gaacaaggta aaaagggttc tagatatata
    15541 aaaaaactaa tacaagcagg agctatgcca atcgtaaatt tttgtatagg agtcatgctt
    15601 ataagagcaa gtgtaaaaat gaatgattct gaaattaaaa aatgccttga agatgcaaat
    15661 aaaattaatg atgtgaaagt atatgataat caggtaaaag gtgtatatta tattgaatat
    15721 aacattaaag ataataaagg cttgaaatca aaaggaattg taaaagcaca tgttaaataa
    15781 gtggaaaaaa taagtcccct gggggctttt tctaagcatt taattaatca atagaaagag
    15841 aggatttaaa ttctgatgga agagggttgt attttaaggg ttgctttaag tcttgcaatt
    15901 tgtacaatag tgtatatgtt attatttaat aataaattgc ctaatgtatt tttttacttt
    15961 tctatatttt tattggcaat ttctttgtta ggtatgattg cattatccaa caaataagaa
    16021 tttaagtaag taaaaaaaga tgtccccggg ggacttttta aatgagaaaa atttgaaagg
    16081 gggtgtttgc aaatgttttg taatagtgtt tagttacaat taaaaaagtg aaattcgtta
    16141 tatcaaagaa gttccctagg ggctttttta agccttgaaa tttattaaaa ataattataa
    16201 ctaataaata gaattgatta ttcttttagt aatattagac tttaactttt aatactttta
    16261 ttggtcaaca atagttatcc ataaaagtat tagtttttat aggtaagaga gtagtcaatt
    16321 aatgatggtg tcccctgggg gcactttttt aatgggaaaa attaaggtag gtgatgtaaa
    16381 ttggctattg ataaacaaat taagagtttg gcaattagta ttttagaaaa aaacaatata
    16441 gattatgacg attggataaa tgaagaacat aaaaaactta tttttagcaa tacaacagtt
    16501 ttacaaaagg caatagaaga aagcaaaaaa aatgactaat atagttaaag gagggagaca
    16561 gcgttatgga gggaaatgtt gtaaatctag taaatgactt aattaaaagt ggacaactta
    16621 ttttggcagc tgttgcagcg ttttgctatc ttgttggagc atatcatcag attagtggag
    16681 gaaaagaagg ctttccagtg gcaaaatctt ggtataaaaa tacaaccttt ggtttagtta
    16741 taggaatgtc cgttatgcaa cttgtatctt ttttacaatc aaagataaac ttttaattga
    16801 tatattttat agaatagtaa atgtagtctt aaaatatttg agtaaattta tttaaggcta
    16861 catttaactt gtttaggtga aaattatgaa tctatttgaa aatataaaga aaaactacaa
    16921 tgagttttta gatacaccaa atgctgaaat gaaacttgct aagaaattag ttaataagga
    16981 atatgttaaa aagattttta ttgttggttt agttgcaata actttgatac ttaatatagc
    17041 aatagaatta attacatctg tgttaggtat tttaagtgga tataaaataa atatattcag
    17101 aatatttatt ccaagatgga taacatgttt tcctatatat tttattgtat atctattatt
    17161 tattatagtt acaactaaaa tgattgttaa tttaagaata tcatttaata aattagaaga
    17221 aggtcaaaaa ggtacagcaa gatttgcaac acttgaagag attaatgcac aatatagaag
    17281 tatacctaaa agggatatac cttttgaggg attaggtgga ataccaattg caagaaatct
    17341 tgtaaaagat gaaatataca tagacgatag tatagttaat aatctaatta ttggtatgac
    17401 aagaagtggt aaaggagaaa cctttattct accttcttta gatatttatt caagagcaaa
    17461 aaataaaatt tctctggtgg taaatgaccc aaaaggtgaa ttagcaataa tgtgtaagaa
    17521 aatatttgaa gtaagaggtt ttgatactta tttgcttaat cttattgagc ctatgaactc
    17581 tatgagttat aatccactac aactggttat tgatgcatat aaaaaaggag agtatgatga
    17641 agcacagtta ctatgtaaga cattaacaca ttctatatat tataatccaa attctaaaga
    17701 cccaatttgg gaaaaatctg ctatgtctct agtaaatgcc ttaattttag ctatttgtga
    17761 taaatgtatt agtgaaggca gtgaagaaaa aattactcta tatactgtgg caaatatgtt
    17821 gtcagaattg ggttcaaaaa attatgtaga tatatatgat gtaccacaca atgctttaga
    17881 tgattatttt caagaaatgc ctaatagatc agtggcaaaa atgcaatatt caacaagtaa
    17941 attttcagca ggaaatacta gagcaagtat atttacaaca gctatgtctg agttgcaagt
    18001 atttacattt aataaattag caaaacttac atcaaagact tcaataaatt ttgaggatgt
    18061 agcaagtaaa gattgtaaac ctagtattat atttatggct acaccagact atgatacttc
    18121 aaatcatttt gtagcaagta tatttgtaag acaactatat tatgtattag ctaaaaaagc
    18181 aactttttca aaaggtggta aatgtgaaag aagagttaaa tttctacttg atgaatttgg
    18241 aaatatgcct tctatagaag gtatggctaa tataataact gtttgtctag gaagaggtat
    18301 aagttttgat ttagttgtac aatcatatac acaacttaaa tcattgtatg gagattctat
    18361 ggatactatt aagagtaact gtggaaataa aatttatata ttaacagatg acccaagtac
    18421 attagaggaa gtatctaaaa ctattggaga aaagactata attacaaatt caagaagtgg
    18481 agaagtattt tcaacatcta aaagtcaaac agaatctctt gatagtagaa gattactttc
    18541 tccagatgaa ttagcaaaac ttgaaaaagg cgaaatggtt gtgataagac ctattaaaag
    18601 ggaagatata aataatgaaa aaataaaagc ccatccaatt tttaatcaaa aagaaagagc
    18661 aatgcaacat aggtatgagt atctactaga tacatttaat aaagaagggt taacattaga
    18721 agatgtaaaa atagatacat tacataaaaa tgttaatgtg gaagatttat taatagattt
    18781 tgaagagaca agtgtggaat tagatgaaaa aatagagaat ttacttaaaa agaaattaaa
    18841 tcctgaagaa gaaacattaa aaaaatataa tgaaagagaa atagatgata ttgacctttc
    18901 agattcagaa aaatataaaa ttataaggat atgtaaagaa tgggatgttg atagtagtga
    18961 tatagaacat ataaaaaatt taaataagaa aattgatata tataactatt tagaaacaat
    19021 aggaagtggc tatttatcgg atttaattga agaatcttaa atgtcacaac ctttagaaat
    19081 atataaataa gaatctttaa aaagtgtccc ctggggacac tttttagtta gaaaggattt
    19141 gaaatttttg gatgaagtaa aaattataga aattttagtt aatttttcac cagttatgca
    19201 aataagtgat attctttcta gtttaactag gacaattgga tggtggctta taaatgcatt
    19261 aacatttatt tctggtggaa tggaaacagt agttgataaa atatactctt ctatggattt
    19321 tttcaatagt gaaggtatta gccagttaat aacacaattt aagcctttaa tatgggctac
    19381 tttaggggtt tcgattttat ttgtagccta tcaattaatg ataaacagga aatttcaaaa
    19441 agatggtctg ttagtcaatg tacttttatc tattatggtt ataatgatgt tgccatcatt
    19501 aatggttgat ttaaataaaa tcacaaaaag tgcaattaag tctgtaaata gtccatacaa
    19561 aactactgct gaacaaataa taaaagataa tttaaccgat ttatattatt tagataaaaa
    19621 taattttgat agtttagaaa ataaaaataa tataccagaa gaattagtaa gcaaaatctt
    19681 tataaatgaa gaagttgaag atggtaaagt tgaaaataaa gatgttttta aaaataaact
    19741 taatatagat actgatggag agaagaaggt aacaaagtta ggaaaagtat ttggtgtatt
    19801 tagtgaaaag tattacagat accatttaaa cttttttata ataataatta ctttagcatg
    19861 tacaacagta acacaattat ttacagcaat taaagtagca cagttaattt ttgaattagc
    19921 atttacaaag ttgtttgcta ccatgtatgc ttttgctgat atttctagtg gtcaaaggtt
    19981 aaaggatata gttaagaata tactttctat atttgcagta atattttcta ctgcattact
    20041 tttaaaatta tacctgcttt atagtaattg gataacaaca attgataatg ataatatttt
    20101 tttaaaaata tttctaatga ttggtggggc gtttgttgtt atagatggac ctaatataat
    20161 tgaacgtaca cttggaatag atgcaggaat aaaatctggt ttgggagtgg tcacaggtgc
    20221 ttattcttct atgaaaatgg gtggagaact agcaaaagga cttggaaaag caactggatt
    20281 tgtagcaagt gcaacagcag caggaactgg tattgcatca ggagcatata gtgaaatgaa
    20341 agatttgaaa tctaacaatg gaaatggaga agaaaataat agtagtactg gaaataacaa
    20401 aggtgggtat tttggagcag acaaagataa ttttaaagat gagacaggct caccaggaga
    20461 aaaatttagt aaagaacctg atgatttaga acaagaaatg aaaaattcca accctataga
    20521 tatagatact atgttagaaa aatcaaatgg tgaagatatg gttgatggtt caattcctca
    20581 aaatgatatt ttagatggag agttagataa atatgaagat ttaaataata aaggcatgaa
    20641 tgataattta caacaagata atatagacag taatatggaa aaagatatgc aaaacttcaa
    20701 gttagacaca gatgaaatgt ctccattaca aagtaaaata gcaagtggaa taaatagaaa
    20761 tagagcaaat tttggaggag gagatataga agatattaaa ccttatgaag attttggtag
    20821 aatgacagga aaagttaatc tacctaatgg gggtctaaat gatgataaag atgaaattga
    20881 cattggaaat attaatctag gttcagaaaa aagcaaaata aatatgacac aagatgtgaa
    20941 tgattttaac gaagaaataa agacaagtgg tatcaatata gatgaaagaa taaatgggaa
    21001 taaaggtaat atatctgatg aaacgtatgg aaaaacactt ggagattcta ttaaaaataa
    21061 tcaaactgtc aataaaataa aaacaaatta tgatgttgga aaaaatacag gaaaaaaatt
    21121 tgctaaagac atcaataaaa taagtaacaa tataaaaaat tataaaaaat aagattttaa
    21181 aataccccta gaggatattt tttaaggagt gaaaagattt gtattatata ccagaagaaa
    21241 tacgttcaga aacaaaaatt tttaaatcat tatatttatc agatttagcg tttcttgtag
    21301 caacattact tttagcttgg atattgagtt ctatagtata ttctccttta gtaattccat
    21361 attacatttt tacaatagta tctactttgt ttttacttgc aaaaagttct gacaatccag
    21421 gttcaaggaa ctataaaact atatattttt tactaacaaa gagcagacat gtttatcata
    21481 tagaaaataa tgctgaaact aaaatacaat taattaatta tgaagagttt aagaaaaaaa
    21541 tagagaaagg tgagaaatat atatgtcaaa aggaaataag gatgataaaa gaataaaaaa
    21601 agaagagaaa gcatttttaa aagcagaaaa gaaaaagaaa agaaaagtag aaaaaaatat
    21661 agcaaaactt gtaagtgatt taattccaat tttagatata aatgttgaaa gtgagtcaat
    21721 tgtgactaat tatggttgta tggacatttt tcaaatatct agtaaagata tttactcatt
    21781 aaatgaagat gaaagtaatg cacatatagc tacctttgca agatttctta gaacatatca
    21841 atctccaatt aaatttattt ttatgaattt tccaattgaa acaacaactc aacaagaatt
    21901 tttaataaaa caaataaaaa aatgtaaaaa cgaaacatat gaagttttgt tgagagaaaa
    21961 gttaggtcaa cttatgtatt tggagagaga taaaacgaat aaagaatttt ttatggctat
    22021 ttatgttgat aatgaaaagc aaagatatga gaaagaacat atgatattaa gaatttcaga
    22081 taagttttta gaatttaaaa aaatatctcc tgcgaaaaag aaaaaaatac tttttaaatt
    22141 aggtaatcaa aatactaaaa taatataaaa agagaggaaa aatatgagaa aaaatatttt
    22201 taaaaaagat aatttaaatc cattttttct aaatgatatt caaccacaag gaggaatttc
    22261 atttaaagat agagtcattg ttaaagggga tggatatgaa acttgtattt atgtatattt
    22321 ttatccaggt caagttaatg aattttggtt aaattcatta acgtcacttt ctagtttaga
    22381 taatgcaata attacaattg atgtaggtac agaggataaa caaaaagcat tagaatctat
    22441 caataagtct ttaatagagc aagaaagtag atattatgaa gataaagata agataagtca
    22501 aataaaagca aaaaatactt atggagcatt acatacttta gctgataatg ttacaaatca
    22561 aggggaagca ataaaattaa ttcaagtaag attttatata tcagataaag aaaaagatag
    22621 tttggaaaat aaagtaaagg atataatgca agaattagaa ggtattggat ttagaggaac
    22681 tgtttgttta aatgaattag agtatgactg gcaatcttta tactatggac ctatagaaca
    22741 aaaaaaattt aagaataaga gaaaagggaa agctgtacct gcatctagtt tagcaggagg
    22801 atacccgttt cattttacaa gtcttaatga tccaactgga atactattgg gacagacatt
    22861 tacaggtgga aatgtgattt ttgacttatt tacaaaaaca gatataagaa aattttataa
    22921 tgcagtgcta tttggaacaa tgggtgtagg aaaatccaca actttaaaaa aattactttt
    22981 aaataacgca ataattggaa atatagtaag gcttttagat ataacaggtg agtttaagcc
    23041 attaataaca ttattaggag gtagaattat ttctcttgat ggtacaggag aaataataaa
    23101 tcctcttcaa attttatcaa ctatgattga tgaagagact tttgaagtac taaataaaga
    23161 atcatttatg atgcatatgt ctaaaatgag tatgatgtat aattatattg ctataaatcc
    23221 agaagttgaa gaggtaagag aatttgataa attattaagt aagttttata atcattttgg
    23281 tatagaaata gaaaaagcta cagaatatga atcaaaagaa tatcctatca tgagtgatct
    23341 tttaaaattc gctaaggaag agatgtatgt ggatgtgaaa aaagaagaaa taagaaaaaa
    23401 tcttacaata gataaacaaa gaagattaga aaatataata cttaatttgg agtctattgt
    23461 aagagattat ggagcattat ttgatggaca ttcaactata acagatataa caaatgaaca
    23521 aattatatca tttcagttaa gaaacttaag tcaatatgat aaaagaatat ttcgtacaca
    23581 aatatttaat atcttaacct tattatggaa taatgcactg aaaattggga caaaagagaa
    23641 gaaaagtttt gataatggat taaaaacatt ggaagatata atgcattatt taataattat
    23701 agatgaagca catacaataa taaattctga aaatatcatg gcagtagact atttgataac
    23761 atgtgaaaga gaatttagaa aatattttgg tggtctttta tatgctacac aaagtatcag
    23821 agatatggtt cctgaaacta tagattctac atcagaagta tttggaaaaa taaaaacttt
    23881 atttgaatta acacagtata aatttataat gcaacaagat agtaatgcta tggcatcttt
    23941 aagaaaaata tttgaaggtc agttatctga aagtgaaata aaatctatac caaatcttgg
    24001 aatggggcaa tgtatattat ctataaatgg aatgtcaaac ataaaattta aggtactagc
    24061 aacagaagaa gagaaaaaat tatttgcagg aggagcttaa aattgaatat taatgataat
    24121 aaaaagaaaa catatacact cttcatcata gtagtgctta tgtttttttt atatactttt
    24181 ttatcaataa aagagaaaag tattgatgag aaacaaaata aagatatagt tgaagttgaa
    24241 cagaatgaaa ataaagattt aaatgaagaa aacaataaac tattaaatga agaaaataat
    24301 attgaagaaa aaagtgtagt agatataggt aataagttta taaaggcata tcatgagaat
    24361 ttaaatagtg ttgaagagcg actagaggct ttaaaatcta ttacagatgt tgacttgtat
    24421 gaaagtattg aaaatgaatt actaatggat agaaacaaag gaaatgggga ttatcagcat
    24481 agagtaataa aacaaataca taaagaagaa tatgaatctc aaagtataga atcagtttta
    24541 tataaagtaa aagtatattc aaattggctt aacaaagata ataaagtgac acaagaagat
    24601 attacgagat attctattat tttagttaaa gaaaatgaca aatggattgt agcagacatt
    24661 aattctgaaa tcctatctaa gtaggtgaga taatgaaatg taaaaaaagt aatatactag
    24721 taaagttact acttgcttca ggagtaggga ctttactatt ttttttattt ttaatttcaa
    24781 caattattct tttattaagt tcatctaata gaggaggagg tttccaaaat gcaaccttaa
    24841 aaggtgttcc agaagaattt atacagcatt ttaatgaagc agcagaagta tttaaagtgc
    24901 ctaattggac tctagcagca attgcaaaac aagagagtaa ttttaatcca agttgtgcat
    24961 atggtggtgc ttggggaatt atgcaaattc agaaaactga ctggagttat tatataggaa
    25021 atggtttagg agacatatat aaagaattgg gttactcata tagttcagct gatgatatgt
    25081 ggagtacata tttaaatgat gtaagagcac aaatattggc tggttcttgg gaatgtatga
    25141 gatatacaaa tgttgttttg aaaaataaag gcattatatc aaaagttcct tataaagaca
    25201 ataaaaaatt ggctgaaaac atgggactta ttgattggta tgcagatgaa aatgattcac
    25261 aattcagaga aatattaaga agaagttttg cttgttataa tggaggtcct ggttatggat
    25321 tgtctgttaa tttagatact gcacaaaatg attatccaaa taaagttttc ggatatgcaa
    25381 tggaatttag aaatacaggt cttgttggag atattatagg aggagatgtt gctgtatctg
    25441 attctgagaa agttaatgct ataatttctt taatgcaaca acaattgggt aagccgtatc
    25501 tttggggtgc tacaggtccg aatcgttttg actgttctgg tttgatgctt tattgtttca
    25561 aaaatgcagt aggaataaat ttaccaagaa cagcaaatgc acaatataaa gcatcacaac
    25621 caatctcctt tgaagagtta caaccaggag attttgtttt tagactatca aatggagttg
    25681 ctaaacatgt tcaactatat attggagatg gaaaagtaat acattcacca caaacagggg
    25741 atgtagtaaa aatatctaat gtgccacata aaagtgataa tagatatgga agatggagtg
    25801 gtttagatta aaatatttat ttaaatttag gttataaaag tgttcctaga ggtaactttt
    25861 gagggaggta aaatgaagaa tattataaaa gaaaaactta atgatagtga atttatatgt
    25921 aaaggaggat ggaaaagtgg gaatacaata atctttgatg atggagcaga gagaaatata
    25981 gattataaag aaattgaaaa tacagaatat gtatcaataa ttctaactcc aaaagatata
    26041 gaaaaattaa atatagaatt aaaaactaaa tgttataagt atgaatatga ttgttaattt
    26101 taaaagatgt ccccagggga catatagata aggagtttaa aacatattat ggaagaatat
    26161 tatattaaat gtttaaatga agaagtagat gaaataagat ttttatataa aaatttcgct
    26221 atcaatttaa atgtatctga aaatatttta gatagaaaaa ttgtatatgt gaaaagtgga
    26281 gcaatcctta taggtgcatt atatggattt gtaagacaaa gaaatgaata tagtgatgaa
    26341 tataaaatat ttaaagaaat agaaaaaata ttagattctg acaatgttat aaatttagaa
    26401 aaatctaatg tttattttga acaaattaaa ggtatggcat tgaccttatg gaaaatatat
    26461 ttgaaaaatg caaagacaga gataatgaga acactaatag taaaggaatt aaaatccaat
    26521 ttaaaagatt tttattatga atattctttg aaaatcaata tgaataagat gtataaattg
    26581 gattgatttg acgacaagag taagttgtta gaaattataa aagtgtaaat aacatcaatg
    26641 atgattggag atttaatatg aatatattta attttaaacg aaataaaaaa aatggacgtg
    26701 gtgtagaggt tattaatgaa gaatctaata atagacaaac tatactagaa ctaaaaaaag
    26761 aaaagataaa tttaagaaaa gaaaaaataa atactatatg tttaactaaa aaggagttta
    26821 taaatataga aaagactaag tttgatgttg ttgtagtttt agattgttct ggttcaacaa
    26881 gtaatctatt taaaaatggt actatacaaa atattcttga aaaattatta ccaatatcgt
    26941 taaaatttga tgatgatgga gagcttgatg tgtggttatt taatacaagt gcatatcagt
    27001 ttaaaagtat taatatagat aattttttta actatgttga aaaagaaaat ctttacagtg
    27061 aggttggtgg aggtacttat tattctcctg taattaaaga aattatctct aaaaaaatag
    27121 ataaggaaaa aactaataat ccagtatatg taatttttat aactgatgga gataactttg
    27181 ataagaaaga aactaaacaa gtaattatag aagcatctaa taaacctatc ttttggcagt
    27241 ttgtaggaat aggaagagaa aagtttgaat ttttagagag tttagataca atgactggga
    27301 gagaagtaga taatgctaat ttcttttctg taaacaatat tgaagtgata gaagaaagtg
    27361 aattatatga aaaagtgatg aaagaattgc ctatttggtt taaagaaata gaaaagaaaa
    27421 gaattttata aaatatattg aggagattat tttttatgaa tgatgtgagt ttaattggaa
    27481 gattaacaag tcatccagaa ttaaaatgta aagcaaatgg taaatatgtt ctttatttta
    27541 atttggctgt ggataatatg aaaagtacag attttatacc agtagaagtt catgggatat
    27601 tagttgagaa atattctact gaattagaga aggggcataa aatagcttta agtgggaaaa
    27661 taaatagtaa gaattatggt cctaataaag aaaaaagata ttttagtgtt tctactagtt
    27721 acatagaata tttaacaagt aaaagtgtag aaaacaagag caattataat gaactaaaat
    27781 atactccaaa gccaattaca gatgatgatg aaataccatt taaattttaa atgccagatg
    27841 aaatataaat aaaaagatgt cccctgggga ctttttttaa ttggaggaaa agatatgaaa
    27901 agtgttatca tagcagagaa acccagtttg gcacgtacaa tagtagatgc aataaaagaa
    27961 gattttagta aaaagaatgg ctattacgaa aataataagt atatagttac atatgctttt
    28021 ggacatctat ttgaattgct agatatagag gattatctaa atagagaaaa aacttattgg
    28081 aacattaatg atataccttt tgttccaaaa gaatttaaat ttaaattaaa atctgatgat
    28141 ggtgttaaga aacaatttaa cacaataaaa gaacttgtaa atagatcaga tgtaaatgaa
    28201 gtaataagtt gtggtgatgc agatagagaa ggggaagtca taataagatt aatattaaat
    28261 gaaataaaag tagataaaag agtgaagcga ttatggctac cagcacaaac agaggatgat
    28321 atattaaaag cattaagcga tttaaaggat attaataaat acgataattt atacttagaa
    28381 gggttattaa gaacacgtgt agactggctt tatggtataa atctaaccag acttttaaca
    28441 ttgaaaaagg gtaaaaattt accagttggg agagtaataa ttccaattgt agatgtaatt
    28501 tatagaagag atttagaaat agagaaattt gtttctaaag attattttca acctgaatta
    28561 attatactga aaaatggaga ggagataaga tgtaatataa atgagttgaa atttgattca
    28621 aaaagagatt gtgattcaga agtaattaaa caaaaggagt gtaatctagt agttgaaaat
    28681 atagagataa aagaggttaa aaagagccct aaaaaattat tttcacttga taaacttcaa
    28741 aataaattat caaaagattt aaaattaagt gctgatgaat ctttgaagat tatacaaaaa
    28801 ttatatgaaa aaggatatgt tacttaccct cgcacaaata cagagtatct tgctgaatca
    28861 gaaaaagaaa ggataaaaaa acttataaat atacatgatt ctggaagcaa tatattagag
    28921 atgaaagata aaaagagtat atttgatgat acgaagattg aaagtcatag tgctataata
    28981 ccaactgaaa aattacttaa aaaagaagat ttagatagta ttgaaactag tgtatacaac
    29041 attataaaaa ataggtttat atgtaatttt ttaaaagaac aaactgtttt agaagaaact
    29101 gttacaactt taaaatttgg aaaatatgaa ataaagttta aaggaacagt tataaaaaaa
    29161 ctaggatatt tagcatatga aaatgacttg aaagaaaaag cagttactaa attttctaaa
    29221 ggagaaaaat tagattttaa tatagaaact gtaaggaaaa aaactcaacc acctagacat
    29281 ttatctcttg aggatttaaa taattattta aagaatccat ttagaaataa tgaaatgaca
    29341 gaagatgaag aatacaattt aatgttaaaa ggagttgaga taggtactgt tgctactagg
    29401 gcagaaataa taaataaggc taaaaggttg cagtatattg aagaaaaaca aggtatattt
    29461 tttataaaag agttagggaa aatattaatc aaatctcttg aagatttaaa tattaatatg
    29521 gataaaaata aaactgttga attgtctaaa atattaaaac aggtgtatag aagagaaata
    29581 gaatctgata aagctattaa aatatattca gacgatttag ttaatacagt taataatagt
    29641 aaagctattg aagtaaaaaa aatagattct aatgtgaaag aaaaagaaat tataggtata
    29701 tgccccaaat gtaggaaaaa tatatttgaa ggacctaaaa atttttactg tgaaggttat
    29761 aaagatacaa acaaatgtac tttttatata tctaaagaag ataaattttt tcaaaataga
    29821 ggaaaaaaac taacaaaatc aatggttaag aatctattaa aagatggaca tgtaaaagtt
    29881 aaaggattta agaaaaaaga tggttctggt tcttatgatg caatagtaaa tatggacata
    29941 aaagaaaaat gggtcaattt tagtttgcaa tttgaaaata aaaaaattaa aagataaaac
    30001 atagaaaggt aaaagtttga atggataaaa taaagaaaaa gatagataaa ataataaaaa
    30061 gttctattaa aatttttaat tttttagaga agaaaataca aagaataaat gaaaatagaa
    30121 caatatctaa taaatcaatt acttattatg cgttaacaat tgctattgta tttggatttg
    30181 gttttttctt taattcaaat gcacttttaa ataaagaaaa agatatgagg tcaactccac
    30241 tttatgaaaa acaagtaata ggaggaatga gtattgaaat tagagataga aaatataatc
    30301 ctgccacatc acaagttcag tttttaattt atgtagataa tgataacaat tttgaaacta
    30361 agaaaatgag ttttgaactt agagaacaaa ataatccagc tgaattaatt gacttaaaag
    30421 caagacaaat tgatgcaaat aattatgtaa tcatttcaaa tgttcctcga aaatggactg
    30481 ttttatcttt aggagtaggt gaaaaagttg tatctggtga taaatataca attatgaata
    30541 aaataaaaat atattctgat attagaggaa ctgaaaaatc atatggactt aaagaaaaga
    30601 atacaaatga atatctagga gatgcaattg ataaagaaat tattgttgtt cagagcaaaa
    30661 taaaagacat agagaaagat tatcttagtg aagaaaatga aataaaaaga atagaaaatg
    30721 atataaaaag tttagaagaa agtagaaaat atcaaactga aagtgagttg gaaaattcaa
    30781 attcagaaat aaatagtttg aaaaattcta taataatttg taaagataaa atgaatgact
    30841 atgataaaca aataaatgaa cttaaacaaa aagtcaagaa acttgaacag aaaaaagttg
    30901 attttttgaa ttaaaaatca tggaaattta ctggaagaaa tatggaagaa acatggaaaa
    30961 ataaaagttt tgatgccccc aagattttac taggctttgc ctagtccccc atgctttttg
    31021 ggggcaaaaa tcccttatgc tctttttttg aggaacgaaa aaaaactacc aattgttatg
    31081 aagttaattt ttattaggag gaataaaaat tgaagccttt aaaaagttta aaaataagag
    31141 atgtaccaga agaaacaatt ataaaattag atgaaatttc acgtaaacaa aatttatcaa
    31201 gagaagaatt tctaagaaga aacttaaaaa caatagctgt ggcagatgaa atatatgaag
    31261 tagaatcgaa atataaattg ctaatagata aagtgttagg tattttaaat ctaaacacta
    31321 ttgtattaaa aaaatttatg gatgaaaatt taattacttt tgaagatata gataaaaatg
    31381 gagagcaact tttgaaagaa atgagtgaga tagatgagta aagtagataa gcattttctt
    31441 ttagaaaaag aaaatgttga atatataaaa aaattaaaag aagaaaaatt tcatacagaa
    31501 agtctggcaa taaatcatat tattaaagag catagagaga aagaaactaa tgatacacaa
    31561 atgattatta gtattatgat agaagaagta tttaatagat taaatcctga actaaaaaaa
    31621 tataatacta aattaaatgc tacagataaa aatgttcaaa taatattaga attaattaat
    31681 ggaattttag ttgaaaataa tttagatgac attgcaatga ctgatgaatt agaatcaagt
    31741 tcgttgttaa aagcgagaaa aacagttaca aaaaagatag aaaaaagtaa atatagtaaa
    31801 ggtaaagcat tatactaatg acttctcctg gagtagtaca tatatctcgc tttgtaacat
    31861 ctactaataa tgcttttaaa aattatatag attatttgga tagaaaagaa gctacaagaa
    31921 atgatgcttt taatagatat tctttatata atgactatat gggtaatcca attaaaacta
    31981 caggtttatt cactaaagaa aaagatgttt tattagatat tgaaaaagaa aagataaaag
    32041 aattatttgt tatggcacaa caaaaaggaa gtataatgtg gcaagatgtt tttagttttg
    32101 ataataaatg gttagaaaaa caaggacttt atcattctgg aagcaaatct cttgatgaag
    32161 aaaagttaaa acatgcagtt agagaatcta tgaatgaatt atttgaaaaa gataatttaa
    32221 aaggaagtgg catatggagt gctgcaattc attacaacac agataatatt catattcatg
    32281 ttgcaacatg tgaacctaat ccaactaaaa aaagaggtaa aagaaaacct aaaataatgt
    32341 ataatatgaa aagtaagttt gtaaattcat tgtttgatgc agaaaaagat tatgaaaaaa
    32401 taaatgaaat aataagagaa aatttaataa aatcaaaaca aaaattttcg tcaacatcag
    32461 actacgaaat gaaaaaaatg tttaaaaagg ttataacaaa tcttcctgct gataaaaggc
    32521 aatggcacta taattataac actatgaaaa attcaagagt atatttagat aaaatgacag
    32581 cgtattatat aaatacatat aagaaaaaag agtacaatga attgctaaaa aaattagata
    32641 aacaagaaga agttttaaaa acaatttatg gagtaggaaa atttgaaaaa tataaagatt
    32701 ataaagcaaa taaaataaaa gatttacaga caagaatggg caacacaatt ctcaaagaag
    32761 ttaaagtctt tattaaagaa aaagaaaaca gagctgatta ctgtaaatct agtacaagaa
    32821 ataaaactat gaatattcat ataagtcgta gggatataga cagattaagt aaagcattaa
    32881 gcgacgaatc aagtcattgg aaaaatatga actattatga aaaactagaa aaagaaatag
    32941 agtataataa tcaaagataa aatataaaag gagtggtttt aatggatttt ataatgtcaa
    33001 ataacaacaa agggatagtt actaatgtac atggagatgt tttaactata gaagatatag
    33061 aaagtaatga tgttacagaa ataattatga aaagaggatt tgttgaagaa agtgaaatag
    33121 aaattggttg cttagtggaa tatgataaat ataaaaagat agtaatacaa gaagatataa
    33181 actagaagaa gatgtcccca ggggacatct ttttaattgt aaaaggagta tgtatatgct
    33241 tttaaaaaaa catatattta aagttaaact tgtaatgctg agtctatttt ttctatttat
    33301 atgtacagtt aaaatagaag catataggga taatgagata gataatttaa aacctcatta
    33361 taatcagatt gaggaattag aatataatta tgagtcaatg attgaggttg attctgataa
    33421 taatgagaaa gtattaaaac tttataaata tatgagttta atacccaata atgttataaa
    33481 tgaatttgca gagactggag gaaaaattgt tttaacacat gaatctctac aagatagatt
    33541 tattagtgaa tatgcattag caggagttta ttttgataaa actatttatt taagtgataa
    33601 agaaaattgg tctgaacaat ctttgataca tgagatgggt cattttatta gtgacttata
    33661 taatgataca agtgatagta tttttttaga tatatatgca aatgaaaaaa actatttaaa
    33721 gttaaattca gataaagata tagactatta tataaaaaat cctgatgaat attttgcaca
    33781 atgttttgaa gagttcataa taaatcctga tagattatta aaaaataatt tgagaacata
    33841 taaatatatt agattttgta taaatagagt ttagtagttt caaaaagatg tccccagggg
    33901 acttttttag gatgtgattt tatagaaata gatgaaaaac tagtaattaa agattttaat
    33961 aaagggttaa gtatcttaga ggatatatgt actttaaatg aattaaatag agttgatttt
    34021 gaaaaaaaat taaatgaagt atttgatatt tgtaatacat tagaagttct tagtgtattt
    34081 atttgtgatg caagtgaaga aataaaaaaa gcattttgtg gtttaatatg tgtcacaaaa
    34141 gcaataaaaa acaacaaaga tgatttttat aagaatgata cttttagaaa aagacaatta
    34201 atatttttaa aaaataatga ggtgaataaa attgaaaaaa tataatgaaa aagacataga
    34261 aaaattaaaa gctaaaatag atggtcattt aaaattagtt gataaaaaat tagaactaca
    34321 tcttaacaca aaagaagaag tggaaaatta tcttaaattt atggcaaaat tttatgatta
    34381 ttcaaatggt aatagaattt taattgaaaa tcaatttagg ggtgcagaag cagtaggttc
    34441 atttaaattc tggaaggaaa aagggtttgc tgtaaagcga ggagaaaaag gtgcaaaaat
    34501 tcttgtacct tgctttggta aatatttgat gtatgaaaat ggagaaacaa aaaatctaaa
    34561 gtatgcatca aaagaagagt tagaaaaagt taaaagaggt gaaataaaaa caaagaaaaa
    34621 actgtgttat tatatggagg gaaatgtatt tgatataagt caaactactg ctaaagccag
    34681 tgatttgcca aagatttttc ctaatagatg gttagaggga gatataagta actatagtct
    34741 aatgtataaa gctatggaga atatagctga gaaaataggt gctaaaattg tagagccata
    34801 tagagagctt ggagtagcaa aaggtgttac atatggaaat aaagaaattg cactaaatcc
    34861 tagaaattct gaacttcaaa atgtaaaaac tttactccat gaacttgcac atgcaaaact
    34921 tcatggaggt aaaaaacatg agaactacac aacagaagaa agagaatttc aagctgaaat
    34981 ggtagcatat acaacatgta cttatctagg aatagataca agtaaatact cattaagtta
    35041 tataaaacat tggactaaag atactgatat acgtcataaa aaggatttgc ttaaagaggt
    35101 gtctgatact gttaaagaat atatagaaat acttgaattg actttagaaa aagaacgagc
    35161 acttactaaa gctaataata aggataagga attagacaat aataaggaaa aagaaagtaa
    35221 tataaaagat gaaggattaa aactggaaaa tacatatgta aagtttacta gtagcactgc
    35281 taaagaaatt gaaaaaggtg atagttggga atttaatata gcaaatgaac ttattgaaat
    35341 gatggagaag aaatgcagta tagatgaaag taaacatgaa attaaaatta aaatttataa
    35401 aaatgagaaa tgtacacagt tactttcttc agagttaaaa tttaatatag gagatgggaa
    35461 agcaaaagat ttagatgagc gtatatcagc agcagtaaaa aatggtgaaa tgaatataaa
    35521 agaaagttct ttaaaagaaa atattttaaa taaatttaat tctacactta aggatagaca
    35581 atcaaaagaa agattggata gatgtatgta aaatgtcccc aggggacttt ttttatggga
    35641 gggaaaataa tgaaaataaa attttcagat gaaaacttga aacaaaaaat gttgttacag
    35701 gagttacaga acagttatga agtaagaaaa ttgaatcata ttactattaa taaaacaaat
    35761 agtaaagaag aaaaaggcga ccttaaaagt cgttttttta atggaaagat agataaaaat
    35821 gaaaaaaaga taattgattg ctttgtgtaa agtttagtga tttcaagtgt tatgtatcta
    35881 atatttatgg tcctgttaaa aaaatagata aaaaatatat agtccctttt tagggactat
    35941 tttttataga aaaatgagag gatggtgtct atgtataaca agaagataat ttctatatta
    36001 ttatctacag taattgcaag ttcaactagt ttaggtatta cacctagtaa tataaacgtt
    36061 tttgctaata aaacaactta taagaataac ttacaaagtg aaaatgaaaa tatttcactt
    36121 catatgggag aatataaaga tgataatgga atattaagtt ttcaaggtgc agaaggtaaa
    36181 cttaaagaag caacttattt aagatttgac ttcaatagaa atgcgtataa tgatgtgact
    36241 gtcttgtata cattaaactc acaagaagaa agcattactg gtaaaaatat aaaaatacct
    36301 ataaagaatc aaacagaatt agatgaagta ttaaaaagtt taaaaataaa tacaaatggt
    36361 aataaaaaat taggctttaa tgttttatta ggaggattac ccatctttaa aagtgtagaa
    36421 ggtaaaccat ctcgtttata taagccagag agagctattg atggaaataa cgatactttt
    36481 tatggagcta taaatcaaaa tgatagcaca tttactattt tgtttgaaga aaaaacatat
    36541 atagaaaatg tacttataaa agcaaataaa aattttacaa tagaaggttt aaaagataat
    36601 gtttggaaaa ctataggaga tagtacaact gattcaatag aaataaatgg gaagaaaaaa
    36661 ataaatgtag aaaaagatat atatgatgga ataattataa aacaaaaaca ttctggcgta
    36721 gactcttgta caattaatga aattgaattt aacaatggaa gtgtagaaga aaaaataaat
    36781 tataactcaa atgtatgtag tttagattta ggaaataaag ttgttaatga agatggaagt
    36841 atatcattta aagactttaa tgctaaagga aatgaagtta aacttttaat aagttttgaa
    36901 aagaatagtg ataaaaataa aatatcttat atgcaaaata aacaagctgt tgagaaaaaa
    36961 ggggactgtg aaatagatgt aaataatgaa actgagttaa atgaaatact agaaagtatt
    37021 aaaatttact atgataataa gactgaaaat agtgcgagta tttcattaat aacaaacaat
    37081 aataattcta tagaaaatgt accaaaagga attatagcag aaggtggagc atctgatagt
    37141 gtacacacag cagaaaatgt attttctggt agtaatagtt tttttataag taattcaagc
    37201 aatactttaa cattaaaaaa tacaaaagat atttatctaa acggagtagc gtttttgaat
    37261 ggaaattctc aaactattat aaaaggattg aaaaatggta agtggtcaga tataggaaca
    37321 catagctcta atgattttga aatgataaat ttaaattctg gttactatga tgaagtaaag
    37381 tttactagta gttctacact ggatattaga aatttatatt taagatatag tgatataaaa
    37441 gaaaatatta agttagatat aaaagaagaa gtagcagaca aaatagattt gggtaaaata
    37501 gaatattcta atggaaaaat aacatttaaa ggtgcaacag gtaggattca agataaggct
    37561 aatattggta tttcatcaaa aactaatgta gagattaatt atattttaaa tggtgaagaa
    37621 ataacaaaag atggaaaaga gataattgtt gaagatttga ctcaggaaac tttagatgaa
    37681 tttttaagta atttttctat aagttacaat gaatatgaag gttgtgagct agatattagt
    37741 ttattaggta attcaagtac aataatacaa acacaaaatt atactatacc tcaaattcaa
    37801 ggtgatttag aactaaagct aggtaattat gaaaatgacg gaaacggaaa tgtagtattt
    37861 ccaaatgcta aaggcttact atatagaaaa ggtaatttaa atataaactt tgaaacaggt
    37921 tttagtgatg atatagaaat aaaatataaa ttaaaaaata ttgaatacac taaaaaagaa
    37981 aacaatttgt ctatacaaat ttctgaacag gctgaattag atgaagttct aaagacacta
    38041 aaaattagat gtgacagtaa tttagaatat aatttaaatg ttactttagc agaagagaga
    38101 tatattcctt tgggaactac atcagataaa ccagaagctt taactggagg ttggtattct
    38161 ggaaggagta gtactgcaac aataaatttt aagtctcctt taaaaatgag tactgtaagt
    38221 ctaacaaaaa cgagtggagt agataactgt ttttatataa aaggattaaa aaatggaata
    38281 tggaaagaaa tagggcgttt tacaactgtc tatccaaatt atgattattt aaaaacaaga
    38341 gaaattgatg tacaaagaga ttcttatgat gcaatttcta taacttatac agttgcaaat
    38401 ggttggggaa gttcaaatgt tagtaaactt ggatttttag ataaagtttc aagtgatatt
    38461 aaaataaagc cagatataaa tttaaaggtt actttagaag ataacttaga aaaggcaaat
    38521 ttgaagtgga atagtgattt agaagcatat gcatacaaca tttatgttaa gaaaaaagaa
    38581 gatgctaatt ggtatttatg taagagtgta acatcaaata ttaatgaaac agatgattat
    38641 aacataaatg atttagcagc tccttcaatt ccaaaatatg aaatacaatt agatggatta
    38701 aaaaaaggaa tacaattaga gtcagatgat ttaggaacag aatactcata ttatgtaaat
    38761 gcaattgata aaaatggaaa taaaataggg acatctaatg ttgagaacgt aaatgtacaa
    38821 agtgatttta aagagtttat ttatgaagta aataataatg ctacaacacc aattgaatta
    38881 aaaaataaaa gtaatggttt tataccaatt ggagaattaa ttgataagaa ttatctacat
    38941 gttgcttctg tagataatat aggtaatatt agtgatataa aaacaattcc gatattaggt
    39001 ttgtttgtag atggaccacc tattataaat gtacctggag actgctcttt aaaagcaaac
    39061 agtaaaatag atataaatga aggagtgtct gctattgatg aaatagatgg agacttaaca
    39121 agtaaaatta atactaaaat tatatctcct aaaggagacg tcgtatatga catagataca
    39181 aatattttag gaaactggat tatagaatat aaggtaactg acagcaaggg gcaaactaca
    39241 gaacatatta gaaaaataaa tgtaataaaa aatataaact taaaacttaa tattgggaaa
    39301 ctattgacaa caagtaagtt tacacaattt ccaaatgcta aaatagaatt taaagaagat
    39361 gaaaaggtaa atggaattaa gatgtatttt agtaatgagt ttaatgattc tatgaaagtt
    39421 cattatacat taaatggaat tgaaaaaata acaactgata gaagtatagt tatagatgat
    39481 gaattaacta ttaaagatgc tgaaaattta attaaatcaa taaaaattga acatgataat
    39541 agtaagaaat tcaactttaa tatagtttta agtagtaatg atgctgaaag tataatttat
    39601 aattatgaaa ataatcatta ttataaattt atttattcaa atggaattag tttagaagat
    39661 gctaaaaaag aagcaagtaa acaagagttt ttaggtagta aaggttattt agtaaaaata
    39721 gattcagaaa gcgaaaaaga agttttaagt atgttaacaa atcaatcctt ttggaatgaa
    39781 gcatcaacag ataataaaga aaaaaatggt tttgtagtag agtttgggct ggaagataaa
    39841 cttccttctc cagatacaat aaatattttt gattatgaaa gcattataaa tattgaacct
    39901 tttgaatcta aacaagatat agtacttaat actttagtgg ataaaaaaga aaataaagta
    39961 atacttgact ggaatgatgt tgaggatgta gattactata aagtatatca aaagaaaaat
    40021 ggaatgggtg attttgaagc aatgtcatct aataattatg caaaaggtaa aaaagtaaag
    40081 gttttaaata tatatccaac tagttccgtt aaagatacct ttaaaaactg ggtaaccgaa
    40141 tctggtcaag aattaattga tgtagacact gtaagtaatg gtgatttcaa taataatcca
    40201 gatttttact taaaagatga aagtggagaa tataaatatg atgtggttgc ttgtggattt
    40261 gctaatataa ctacaactac ttcacttcca gaaaaatctg tactagctat aaatgatgct
    40321 aagaaaaata atgttggagt gctacttgga caccattata ttggagaaag cataggtaat
    40381 tttccaaaat acagcgaaga gtattttatg aataaataca aaaatacgga tgaggtttgt
    40441 agtcatgagg tggaacttga taaatctcat gaagcactta attatccaaa tgaactggga
    40501 aaagtaggag atgttctaaa gataccacat actcatagta attataatgc ttttcctttt
    40561 gaagatgaca ttgtaataaa atttcataat ccaaaaagac atgcatatcc ttataattgc
    40621 aataaaagtt atgagggata ttcaggagag ataaatggaa aaaatgtatc tcaaaatgct
    40681 tatttaagta tacatgaaaa cacagcacaa atccagacag gacatagtgg aacaaacatt
    40741 acaactgatg aaaaaaatat gttagcaaac acagtctttt atttagcaca aaaattggat
    40801 gaaagctatc ttgaagatat aaatggaaaa gataccaatg aaccaacagc tcctacacat
    40861 aagttatacc aaatagggat aaaacaagga ttaaagtttg attcagaaga tactggttca
    40921 aaaattgaat attatgtaga aggtgtttct aaatatggtg atgtaacaca tacttcaaac
    40981 aaaaaaatgg ttgaaataaa gtctggtctt gaaggtttta gttatgaagt aagtaacaat
    41041 cctactccat catcaagtct aggagacaac ataaatagta cagatggttt tatttctgca
    41101 aaagaattag caagtaaaag atatgtacat ataatttcta tggataaagc aggaaataag
    41161 agccaaatag tttcattacc aataacagga ttatttatag acaatccacc agttttagat
    41221 gtaggtggaa acatatcggt ttcacaaaac gaagaatatg atgttatgaa aaatatctca
    41281 gcagaagatg attatgatgg agatttaact aaagatataa aagtgagtat taaaaatcca
    41341 aatggtgaaa tagctaataa atttgataca agtgttttgg ggaaatggac aatagaatat
    41401 agtgttaaag atagtaaaga gcaagaaagc agggaagaaa agacaataac agtaaaagaa
    41461 aatttagatt atttaaatgt aaatttagga gagcctaata aaacaagatt ttgtacagaa
    41521 tttcctaatg catcaattat aaacaatagt gataaaaaaa taaatggaat taaactgtac
    41581 attacttctg ggtacaatga aagtatagta ataaattaca ctgacaaagg aattaaaaag
    41641 tcttttaagg gtcaacaaat aatattagat gatagttata caacagaaga agctaatagt
    41701 ctagtaaagt cattaaaaat agaacatgat aatacaactg attttgattt taatataaga
    41761 cttggaaatt cattagcaga ggatatagta tataattctc aaaataaaca ttattataag
    41821 tttgtaaatg cgtcaggaat aacatgggaa gacgcaagaa aagaagctga aaaacaagaa
    41881 ttagaaggtg ttaaagggca tttacttact atagaatcat ctgaggaaca atctataata
    41941 aatagtatgt cacctcaatc tatatggatt aacttaaaaa ggctagatgg aaaatgggta
    42001 tctgtaattg aagatgagac aaaagaagca acttatttta attggattga tggagaacct
    42061 aataatcaaa atggaattgg tgtacaagaa gattacgttc aaagtataaa ggagaataat
    42121 ggaaaatgga acgatattcc aaatgatact aaattaccag gattgcttcc aaaatttaaa
    42181 ataaatggat atgtagttga atttgatgtg aataaagaga tttctaatct aaacacttat
    42241 gaagatactg cacatatcaa aaaaatgatt tcaaatcaag atattgtttt aaatactgta
    42301 aatgatgatg aaaataatta tgttaaatta aattggaaca aatataaaaa tgcagagtat
    42361 tataaagttt ttagaagaga attaaatagt gattcagatg aatttgaaac tatcttagaa
    42421 aatacaaaag aaaattgtat taatgatatg gatggaaaag atataacacc tccaatatat
    42481 gatttttctt taatatttga taaagagatg aactcaaaat tagaatttca tgcagaagag
    42541 gttggaacaa cctatgaata taaaatagaa agttatgata aagacaatta tcaaactgcg
    42601 tattcaaaca cttctaaatc tacagttaca tctaagttca aaggatatag ctatgttgtt
    42661 gatgaaaaac cagatacaga gcttgataat accattaata atataaatgg aaaattttct
    42721 tttgaaaatg taacttataa cacatatctt catataaaag caatagatga acaaggtaat
    42781 gctacaaaaa caaagcatat taaacttgct gatgaaacac ataacaatga gcctagaata
    42841 actgtaaaag gaaaaaatag agttattaaa gtaggagaaa aatttgataa attagatggt
    42901 gtaaaagcat atgattttga agatggggat ttgacagata gattagttgt cgaaggcgaa
    42961 attaataatc aggtaaaagg tgtgtatgaa gttaagtata gtgttgaaga tagcaaaaga
    43021 ttaaggtcag aagtaattgt aaaagttcat gttacaggtg atatgaactt aaacatagaa
    43081 gatcaagaaa aagatgaact attaattagt tggaataaag taaaaaatgc tgttaaatat
    43141 aaactataca gaacagatgt aaatggatat gattttgaag aaaaaggagt tttcactgat
    43201 acaacattta tagataagtc agctaatgat gtaagttctc cagtaataac ttatctaaat
    43261 gaagtaatta tagatgaaga aacaagtaaa attggcagac ttactatttt aagtaaaaat
    43321 ataataaaat tagcagacaa agaaatcaat aaagattcag aacaaaatac tgaattaaaa
    43381 ttattagcaa gagatttagc atcaacatat agatacttcg ttgaagcaat agatataaat
    43441 ggtaatgttg taaactccag tgaaattaaa aatggttctg tttcagttgg actagcaggg
    43501 tttaattaca taatagatga taaagaagat acagaagcag acaattttgt aaatgcaaaa
    43561 aatgtacttg atatagatat atctaatagt aaatttaagt ttctacatgt gagagctatt
    43621 gacctaaatg gaaatgtaag tcaaactgca catcacttaa taagtggaaa atatgatact
    43681 aaccatattc ctgttataag aggtttaaat acgataagag ttaaagaagg agataaattt
    43741 gaccaactta aaggagtgag tgcatatgac acagaagatg gagatttaac atctaaaata
    43801 gaggttgagg gaaaagtaga ttcaaatgta aatggaaaat atatattaac atataaagtt
    43861 gaagattcac aaggaaacaa aagagaagtg aaaagaaata taattgtata tgcagatatg
    43921 gaaattaaag taacaccaga ctttgaagaa aataatttag ttatatcttg ggaagagaat
    43981 cctttaaaag ataaagtatt ttatgaagtt agaaaatatg attatgaaat tggaaactat
    44041 aaaactattg gatatacaga agatttaagt ttcgtggata aaaatgcaaa agacatttat
    44101 ggttctaaaa taaataatat acatggaagt gatatggata cagatgaatt agaggataaa
    44161 attatacttg atatagatgc taaagatata ggagcttctg aaaagtatca agttaggact
    44221 agattaaaag aagataattt aattcttaga gatatgacaa atgaagaagg agtagaatgg
    44281 ggagttggag gaaatctgag tggagttgtt aaatctttta aatggaaatt aagcaataaa
    44341 agagaagata atttagaaag tggcatgata actacatcta tatctcctaa taaagtagaa
    44401 tttgaacaca aaggatacaa atatattcat attatgccaa ttgattccta cgataatcaa
    44461 ggagatatta tccattatga aattggtaaa ccacctgttg aagaagtaat tgagccacct
    44521 atcatagaaa taaatggagt tccagttata aatggagtaa gtacagctac aataaaaatt
    44581 ggtgatgaat ttaaaataat ggaaggtgtt actgcaaaag actacagagg tcaagattta
    44641 actaatttta tcaaggtaaa aggatttgta aatagatatg aaaaaggaga atataactta
    44701 acttattcag taattgattt caaaggtcaa gagacttcaa gaacatgtaa ggtaatagtt
    44761 atagactata gtgagcaatt accagattcc aaaccagata aaataaatag ttctccagaa
    44821 gttaattttt ggaatgaaga ttatatttat gtaggagaaa aatatggaga agatagaata
    44881 ttaaacggaa taagagtaat tgatgaagaa gatggagata taacatataa agtaacttat
    44941 gagactgatt taaacataaa tgaagttgga acatatgcaa tactttatag tgtaagtgat
    45001 gatgatggta acaaagtaaa atttactaga gtagtgtatg taataccaaa aggagaatat
    45061 aagccagatt taggaaataa tgaaaactgg aaaaatggaa gatgggcaag aataaaaaca
    45121 tataataagt atctaaacgt aggagacaca tttaatataa aaagtggagt atacgcctat
    45181 gatgatgttg atggggatat aacatcacaa ataaaagcat ctggtatcgt taatacaata
    45241 aaagaaggtg catataatgt aacatataaa gtgacaaact cagatggtaa tacaactgta
    45301 gcaggagcaa caatatttgt aaaagatggt ggatgggaca acaataatgg tgagaaacct
    45361 aatccaaatc cagattgggg aaacaatgat aatgatgatt cgcataataa tcatattcca
    45421 aatagaccaa accagccaga acaagatgaa gatgacataa atggtgataa gcttcctggt
    45481 gtagatgaca atactgacaa agataaaaat gataatatat ttattgataa agatggaaat
    45541 aaaacatatc cagatggaac aattgaaaaa ccaaatggaa cagaagttaa accaaatgaa
    45601 gaaggtgaaa aaccaactat tgatgatgat ggaaatataa ttgttccacc agatggtgtc
    45661 atagtaaaac cagatggaac tgttgtaata ccaccaaatg gagcaattat aaaaccagat
    45721 ggaactattg ttatgaatga agaagataaa aataaagaag aaaataaaca ggaggaagaa
    45781 aacaagcata ctaataaaga aaatgaagtt gataaacaca atgtgataaa tataaaaaat
    45841 gaaaaggata aaaaagaaga acaaaaatct gggcaaaata ttaaccccga gacaggagat
    45901 aaaggaataa tagcttccat tgccattttt actacttctc tagtaggata tttattctta
    45961 aatagaaaga aaaataaata acattataaa tttttttagt ttaaaaataa aaaaagatgt
    46021 ccccagggga catctttttt attgtttatg tttttttatc caatcaagaa tatatgtttc
    46081 atcagcaaca ttagaagcaa cttccaatcc taatttaaca agttcacttt gagaatattc
    46141 tattgaaatt ttatttaatt tgcataataa aagcattgta gaaataccaa ttcttttatt
    46201 tccgtcaatg aaacaatggt tacttattaa actatgtgtc gtaactgata ttttttcttc
    46261 aattgtttta tataagtcaa ctccagaaaa agttacattt gctctattaa gagcactatc
    46321 taataatttt atatctttga caccttgaaa accaccactt ttagttatta ttttttgatg
    46381 atattttaaa atctcatcta atgatagtga aatatacatt attcagccaa ctttcttaga
    46441 gcttcaatat tatcatctat tgtatcatcc attattttat caagattttt ttttgcatta
    46501 aaatttaaaa gttcttttat tacttcttcc ttttcatttt catccaaatt ataaaattca
    46561 tttataatat tttttgctgt agccatagct atcactcctt tttatatctt tattttatta
    46621 tatcataatt acatcattta agatttgatg cattttattc aatcatatta gagttatata
    46681 tgtttattat tagcagttaa atatatttaa ctccactttt tctatattaa taaaatctat
    46741 atttttattt aatgtttcta tatttatatt tgaattaaga tttatctcta aatttgtatt
    46801 aattagattt tcttttttca tttttataat gcacttattt aattcttgct ttgatgaagc
    46861 tattccaatg atattagact cattagaaga gtttccaaaa gtttcagata taatatatac
    46921 ttccattatt aatacctcct aaagtattta atttgttaaa tttaaatttc acatatttat
    46981 atatttttgt aaaaatctat tttattattt ttttttaatt cattatattt attcatgcaa
    47041 gtgctttgag tcctttcaag tgccatactt aaatttttcc aactcatata aggtcgcatt
    47101 ttacataaat agcttaattc ttttatactc taattctttc cgtgtctacc atgaaattct
    47161 ttattatata ccattctgtg attgctttta taatattcaa ttccatcaat gaaataagtc
    47221 tcaatttttt tattatgata tatcatttta tcctccaaaa taattatttt atttattcaa
    47281 ctgaaatatg tactatattt cagttgaata ttacgtatag taatagatta ttttttattt
    47341 tttaggtctc tcatatacat tctaattaat gcatcatcac caagtttcaa agaataataa
    47401 aataaattta aaatgaaatg aacattttct ctagttacta taaaagctaa atttaaataa
    47461 taatttgatt catatttata ttgattttta aaataaggct tatttttgat agtttcatag
    47521 acttctagtt catctgtttg taaattataa atataagcat attcacaaaa aacacctttc
    47581 agtaagaaac ttgaataatt tttaaatttt tcttttttta aactaaagat acttaatgta
    47641 tcattaacat gtgcatctac tgaattatcc cacgtttttg ctttgaacat attaataaaa
    47701 tctttttttg aatggtcgta atatatttct aaaacatcaa gccctttata gataaattca
    47761 cttgattcat ggttataaat tccaaatatg ttatttgtat tatctcttct ttttgtagtt
    47821 ctataaccta ttaatcctct tgtactcata ttattttccc cctttaaatt ttaaaactaa
    47881 taatttatga tatacaaacg tgagttgttt ttttgttgag cattaaatag actatatttt
    47941 ttgtttgtca aaaagtttac tagactaaaa aaatattaag atgttgttta tattaatatt
    48001 tttttagtcg gttttgacaa taaaaaaaat atagtctatg ctgttgcgaa aaaaaaacat
    48061 agtgaagtat tttacttata ataaaaatta tctttacaga gctttttaac attacattat
    48121 ttctaccaaa aaagagtgtt atataattta tattttatat aacactcttt ttttattttt
    48181 ttggagtttt gagaataaca agtaaagaat caattaaact ttctattgaa ttgtcatgta
    48241 tatcttctaa aaattttttt gttgcaaatt ttatatattc ttctttagtt attaatattc
    48301 tataatgtgt caatccattt attttttctg catttaggaa atgcttttca acaagctttt
    48361 ttaaaaaagt gagtgtagtt gttgttttcc aactatattt ttctctcata ttttcagtta
    48421 ccattttaga tgtaattata tcatttattt cccaaataaa cttcattact ttaaattccg
    48481 attttggtat tttttgtata tgcatataat tataatcctt tcttatatac ttatattttc
    48541 aagctagtaa ttatatatat agtcttgatg tttatttata tattgtttac atttaactac
    48601 gtttttcgta aatgtaaatt ttgtattaac tagatataga tgattattat tttttaacca
    48661 tgtaactttc atgtaatttg tccaatatct aggaaataaa aattttttga tttttattat
    48721 ttttcttgga ctttcaaaac aaactagtgt aggagaaatc tttatatttg atttattaat
    48781 tcgttccaat aaatctttcc taataaataa atctggacaa aaagcataaa tatacaatgt
    48841 agaatttcta ctagataaag taaaatattt ctctataaaa atttcaaatt tgtctccttt
    48901 ttcatctagg taatataaaa aatcaatatt atcatcattc gataaacttt ttagtttata
    48961 agcagataca ttttccttta tccattctcc atttttattt atattacaat ctatgtattc
    49021 aacttctatt gtttttttta taactttttt accattcttt gattcaattt ttatcatata
    49081 gactcctttt ataccactta tagatattaa aattataaaa tatatactat atatatttta
    49141 tcataaaata aaaaaagatg tctaaattaa tttaatgaca tcttttttta ttaaatttct
    49201 attttgatac ttccccatat tcaattattt cttttattaa tttaagcata attttttgct
    49261 ctttcagtga taaagcatca atcaattcgt agcactcaga agatactgtt tcaacttcat
    49321 catcatttag tataatattt tcaagtaaaa tttctaatgg catttctaaa gcatttgcta
    49381 tttttaaaag actatgaaaa gatgcctttt tttctgcacg ttcaatttga ccgatatatg
    49441 tataagctac accaactttt ttagctaatg ttttttggct atagcctaat ttttttctga
    49501 aattttttat tctatttcca attataatat atattttatc taaattcatt atgcaccttc
    49561 ctatacacat cataaaattt actttattta taaagaatgt tttattttaa taatatgcta
    49621 tgtatactag aagtattccc taatagatag caattataca aattacatat tataggttgt
    49681 ataagaagcg ttgaattttt aatatatatt attttatgtc cccaggggac ttttttttat
    49741 tattatttta gaccccataa acctctatca attttataaa atattttatt ttcttgaaga
    49801 gtctgcctaa ttttttcttt ccagtttttg tttgaattac attttttatg tccctcgatt
    49861 ttgttgtata tttcatttaa ataagctttt ttattcaaat tctctagtac attagcaact
    49921 aaatccttcc aagttatttt taaactatct cttatatcaa aaaaatcagt tttagttatg
    49981 ctaaaaggga ctataagagg attatctttt cttaatatca aaatatactc atgagctatt
    50041 cttataaaat tattgtttga atatatttta ttatcactca tgcaattatt ttgttgtttt
    50101 ataattattt gctctgttgt accaagttta tttatatcta aaagcataga ataaagcttt
    50161 ttattttttt tcacgtctgc cattaaaata gctattctac cacctttttc aagtgtatta
    50221 aactgttttg tcaaacaaaa atttaacttt tttacaaatt cataataatc ttttatatga
    50281 gataaatcat tttttagtat ttcatttcca tatacatttc ctgaaaattt tactatatcc
    50341 caatatggag gatgccagaa aataaattca tttctttctt taatgtcatt gtcaattaaa
    50401 tcaaatccat tatttaaatc atagcaattt gatgatatat ttaaataatt tgctacatct
    50461 tctgttgtaa aagaaccaga catataatca cttattgaat caaattgata tactttatgt
    50521 aagtctaaaa taatttttcc acttgtattg cctctgtaat tacttctccc aaaattacct
    50581 ctatttttgt agtttacaat actgttcatt ttttattacc accttattaa taaaatattt
    50641 attgttttaa gttttaaatc taaagtggtt ataataacca ctttagattt aatttttaat
    50701 aacaaattta aaataaaagt tcattttcct taaatgaaaa atatgattca tctgaaacaa
    50761 tgatatggtc taatatattt atacctaata aatttcctga atctattagt cgatttgtaa
    50821 tgtttttatc ttcttgtgat ggtttacaat ttcctgatgg atgattatgt gaaattataa
    50881 ttttgctaca attacttaaa attgctattt taaatacttc tcttggatga actaaactag
    50941 aatttaatgt accaattgat atagtagata taatgcaaat tttattatta gtacttaagc
    51001 ccaatattat aaaatgttct ctgtcaggat tatctaaaaa atttaatatt actttataac
    51061 aatcttttgg ttcttttatg tgtatattgt ttacaaataa atctttttct ttaacaagtt
    51121 ttacttttac aatttcaatt tttttcatat taagaacctc ctaaatatca actcacaagt
    51181 tgtttttgtt gagctgaaga tttcatgttt gcattatttt atcaaaaagc tttgctaagt
    51241 ataaattttt ttggccaatg tttttaggct aaaaaaattt atactgcttt tgataaaata
    51301 aaaaaatatg ataagctgag atgacaaaaa caagagtata atacattact taaaaggaaa
    51361 ttctttactt tgaattagaa atgaacaaaa aaaacaaaga ttactattat gtatttgaat
    51421 agaatctttg tttttacatt aaattattta gagttttctg ttgctaatat tttctaatgc
    51481 ttcaagagct gttagataaa aaatattttt attatgtaat ttagttatat atatacaaaa
    51541 tatagctaat gtcgctaaaa taaataaaat attatataat aaattaaaat tactatactt
    51601 tatagaaata aaacttatag aacatatata acttatataa aatgaggtta aagtatattt
    51661 aattatttta aagttgtatt ctaactgtgc tagatgtgtt ttaattctaa ctttttcaat
    51721 gcttatgttt ttcttataac gcatatcatc aagactgtta taaaacttat aatttaaatt
    51781 ataatacatt tgaatttttt tattagtctt attataaaat aattgttttt ctatcaaatc
    51841 atcatctcct ttaaatatga taaaacagac tcaacaagtc tgttttatca tatttaattt
    51901 agttacattt taagaacttg agatataaaa tcgccctcat catctaaaag tttatatggt
    51961 tcatattctc tatatgacct tatatcgcct agaaccacta tttgtacatt ttttcctaat
    52021 gtagcaagtg ataagtactc tgttaatgca ccaagtttgt ctccttgttc agttcttaaa
    52081 gctagacaac ctatgttatt aaattttttc gctatcacat aacacatctt ctatcactcc
    52141 cttaaattct tctatatttt taaagttacc ctcgtcaaat aatttcctaa cttcttttcc
    52201 aattaaatta ttttttgtat gtttatagat atacatatgt aataaatcgt ttatttgtct
    52261 atctacctta gtatttattt gtaattcgat tggataatga aaatttgatt tttgataata
    52321 cagatgaact cctctatatc catcatcatt agcttttcca gttctcatat ctgctaatct
    52381 aaaagaagat aaatcttgtt caaataattc gtaataacta tctactatta ttcttattcc
    52441 taataaatca ttaaagcatt tgtttaattc catatattcg atatgaaaca ggaaatttaa
    52501 aatctatttt tctagtattt aaatatctac ttatatttct tatttcttta aatatatcat
    52561 ctttattaaa cttccttaaa gtgtttttta aactttttcc tagttttgat tcataactaa
    52621 gttcatctaa tatattctct gtaataccat aaaagttaaa tttcataaaa tatcccctta
    52681 cttttctttt ggttaatact tattatatat aattataaca taaagtacaa taaaaagcaa
    52741 tccttaaagt gtatgcaaag taaaaataaa tacaataaaa agattgctaa aaagtgtata
    52801 ttatgatata atataaatat agagaggggg aagagtaata attagtaaaa ttgatactga
    52861 aaaggaggaa aatagattat tgatgggaaa gagtaaatta aaaataaaaa agtgccctca
    52921 gaggacacca tttgattaaa taaactttgc acggttttat ttaattattc taatataagt
    52981 ataagataaa agacaagaaa aatcaatata aaaaggagat ttacaaatga aaactatatg
    53041 tgtttataat ataaaaggtg gtgtaggtaa aactacaaca actataaata tttcagcaat
    53101 tttagctgaa aagggatata aagtattaat ggtagatgca gacccacaag ctaacctatc
    53161 gaatacatct gaaaattata atcataaaga ttatacaata gcagagttac taactaaaaa
    53221 aggtatgttg actaatgaag taataataaa aacagagttt gataatattg atttgataac
    53281 atcagatata agattttctt attctgaaag agaaatattg cttgatatgg gaagaaaacc
    53341 acatgataga ttaaagaaag ctttagaaca agttaaagat gtttatgatt attgtattat
    53401 agattgtcct cctgcttgga atttaataac aataaatgct ttacatgtaa gtgatgaagt
    53461 attaattcct attgtaattg atagatatac acttgatgga gtaaatgatt taatgcaaaa
    53521 aatcaatgaa attaaggaag aatataatcc taacttaact ataaaaggat gttttgtaac
    53581 tagagacgag ccaacaactg taaataagca agtaaaagaa ggattaaaag agtttttagg
    53641 ctctattctt ttgagaacaa gtataagaaa aaatgtatct gttccagaat caacttttga
    53701 atgtaaacca gtaatttttt ataagaaaaa ttcaaacgca agtaaagatt attatagttt
    53761 agcaagagaa ttaaatttat aagaaaaggg gtattttata tatggctaaa aactttatga
    53821 aaaataatgc agtaactaaa ttagaaaata taaaaaatga ggttcaaaaa tctgaattaa
    53881 aagaacaaga gcaatttaga atagagtata tacatataga tgatattgtt ccaagtaaaa
    53941 agaactttta ttctattgat gatgaaagta taagtgaatt agctgatagt attcttgaag
    54001 ttggattgct tcataacctt ttagtaagac ctaatatcaa agtttttgaa atgattttaa
    54061 gagtatatat agctgactta gaagcatatg aaaatggaga attgattggt aaatggttaa
    54121 gattacccga agatgaagaa aaaatagaaa aaatcttaaa aggtatggga aataaatact
    54181 ttattaagga ttatattaca aatatagaag gtttagacat agaaggtaag ggaataaatg
    54241 agttaaatga actagcaaaa aataatcaac ataaaaaaga gtttgaaatg atgttaaaaa
    54301 tagaagtatc aaatttggat acttatgaaa atgctaatga atgtactagt aaatggatag
    54361 atttaccatc gaaagaagaa acaataaaag aagctttaga atatgttggg gatagatatt
    54421 ttatttcaga ttatgttact aatttaaata taaaaggact agaagataaa aaaataagtg
    54481 aattaaatga attagcaaaa aatctaattg atggattaga atatgaaaaa tatgaattat
    54541 taagtggaga aagacgttat agagcacata aaagattagt agaactagga tataaaaagt
    54601 ttcaacttat accatgtcaa gttatgagtt tagatgatgt agacgctgaa atagcattaa
    54661 tttcagctaa tgcacataat agaccattaa ctgattatga aaaattaaaa gaaacacaaa
    54721 agttaattga tttacataaa cttaaaaaag ctaatggtga aaaggtaaca ggtatacata
    54781 atgccgtatc tgaaaaatta aaattaaaac ctacacaaac taaaagatat gtaacaatgg
    54841 ctaaaaattt aattccagaa cttgaaaaaa tgttagaact tgagaaacta tcggtttcaa
    54901 atgcaagtat attttcagct ttatctaagg agaatcaaag agctttatat caatcactta
    54961 aagataaaga tgatatgtta gaaattacta aaaaagaagc agaggatttg aaaaatagat
    55021 ataaagattt agaagaagaa aagaatataa tagaaaaaag ttatatagat aaaatagaac
    55081 aacttacaaa agaaaaagag ttaaaagaaa aagagttaaa agaagcaact aatggtatta
    55141 atgaaaaaat agaaatgata aaaaaagaaa tggaaacaaa aataaaggta aaaacggatg
    55201 gagaaattgg agaataccta gaagagatag aacaactgga aaaagaaaaa aaagagttag
    55261 aaattagaca taaagaaata aaagaagaac aggaaataaa attagaaaaa ataaaagaag
    55321 aacaacaaga attagtatta aaggctagag aagaagtaaa actaaaagca gaacatatat
    55381 tacaggattt tgaagaggat aagaaaagat taaaagaaga aaatgaaaaa ataaaattag
    55441 aaaatgaaaa gttaaaaaaa gtgttagata gcaaggattt tgatgataga gagaaaaaag
    55501 ctatatatga aacaaagata actttaacta aattaaaatc agatgttata agagttacat
    55561 ctttaatgaa aaatatacca tcagatgaag aaatattaaa agatatagaa tcattacaga
    55621 ggttatttaa gacattagaa tctacaatag aaatacatgc aaaataaaag atggacaaaa
    55681 agatgtgtcc cctgaggaca catcttttta agtaatatgt taatagaaaa ccatttaata
    55741 tataaaagaa taacaagaaa gtttacactt tgattttttt atttaatgtt atgcgtagca
    55801 tagtgtttct aatagccaga ataaatatgt attttactat acaactatat tactattaga
    55861 aaaataaaaa agttaaagtg tctgttgccg acaaggttaa tatagatatt aaagctaaaa
    55921 gtaaaaagag gtggataaga aatggatata atcaaacaga taaattataa aaaaaatgaa
    55981 ttaggaatga atcaaataca atttgcagtt tacttagatg tatcattgaa tactgttatt
    56041 aagattgaaa aaggagaaat gccttctcaa agaattttaa ataagataat gaaaaaactt
    56101 aatatagaga attataaata tgcaaatgaa aaaaaaagga ccatatactt tcttaaaagt
    56161 gttgggagat taaatatacc ttctaattgg actgaatgta taggagttac agaggaaaat
    56221 ccaggtgtta ttttgtattt aattgaaaat gaatctaatg aaaatgagat atggatatta
    56281 aatgataata tgattgaatt tgatgatatt aagaaaagtg ctgaaattga attagaatca
    56341 atagaaaaac ttatgctttt taatagtaat ggaaaagggg ctataatacc aaaacttagt
    56401 gtacctttgg attggttaga aaatatgaat gtgaaaatag agtctccatg tgtttctata
    56461 cagctatata aagatgaaaa tatatataaa agtaaaatag taataaaaaa agataaaata
    56521 aattaggagg atattttatg aaagattatt caattaagaa cacaactaaa aaagaaagaa
    56581 aagaaatagc tgctggtgcg ttaggtttat gtatgattga ttcaaaagaa cctagtaaag
    56641 aaatgaaaga aatagtaaat aaatatgtag aaggtgaaaa agaaatatca gaaatacaaa
    56701 aagaagttct tgaaatgtat aaaaaaagat ataaaagagg taattaatat ggaagatata
    56761 tatttatacg aaaacacttc tgtacttaaa aataagcttg atataagaga taaaaaacaa
    56821 ttggaggatt ttgaaaattc cattacttat atcaaattgt taaatgtaga tgaagttatg
    56881 caaaatagag aatttaactt cgattatata aaggatgttc ataaacatat atttgaagat
    56941 gtgtatgatt gggcaggtga accaagaaaa attaatattg aaaaaagtga aaaagtgcta
    57001 aatggtatga gtgtcgaata ttgtgattac acacaagtag aaaaagaagc caacaaggct
    57061 atagaagaat taaaaaacat tgagtggaaa gaattagatt tagatgaaag atcagagttg
    57121 ttttctaaga aaatctcaaa tttatggaag gttcatgctt ttagagaggg aaatactaga
    57181 acaataatga cattttctgt acaatttgca aaagaacaag gatttcacat ggatagtgaa
    57241 atatttaggg tatactcaga gtatgttaga gattcattag tgttggcttc aattggcgaa
    57301 tattcagaat atgaacattt aacaagcata gtaaaagatt ctatggaaat aggagaaaga
    57361 aaatttttaa gaagtaatac atatgtaaaa ttcaattcat catttaatga aattaatgaa
    57421 aatgatgtat ataaatttga agatgcagaa gaactaatta gtgtattatc agagaaatat
    57481 aattgcagcg aagaaataaa aatatcaata tttcttgata aagcatgtaa gaaagaattt
    57541 tttaatggat gtattgattt ggcaaattgt aaagaaaaat tatcatatat aattaaaaaa
    57601 gataaacctg tagtaaagag agaagaatta gaaagataaa agtttttata ttataattaa
    57661 aaggagtttt gtactaatgg aaaaacattt attaaaatct gtcttaaata gaatgactta
    57721 tggagatatt attaatttta aaatgcatga taaagatgaa tataaagagt ttatatccat
    57781 agaagataca agggcatggg gcaaatctaa ttatgaatca tgggcaagta aatatagaga
    57841 aattatgaaa ataaataaat gtgcaattga aggaaatatt ttcacatcat taatagaaga
    57901 ttattgtggt catgcatata gagaaattaa taaatattta aggaacagat ataaaaccaa
    57961 tgaaaaagaa gtagaagaag tagaagaaga agcaaaaaaa tacatgtcta aagagctatc
    58021 ttttatattg gcaatggtat taagtactgc acctagaatt tcagaaaata tagttactta
    58081 tagattagtt aatgactatt ttattcaaga attgatttca gaaaacaaaa agggtctacc
    58141 tgttatagaa cgaggctttt taagtacaag ccttctaact gatataatag gagtaaagga
    58201 agcatatcat gaatatgata atttattgaa aatatatgta gatagaggga caatcggatt
    58261 atatgtaaat gaaatttttg aaataccaga acgaagaaga aaagaagaag aaatactgtt
    58321 ctatccaaat ggatatttat atttgatgaa atatccatat aaagatagcg tactaaataa
    58381 aaaagtatat gaatgttatc tatactatta ttaaaattgt taaaattata aattgtatta
    58441 acagttttca actaagttta ttttttaaat ttattatatt atagttcgga aaaaccaacg
    58501 tttaaccgta catgaaatat ttattctatt ttcacataaa atcattggaa attaacaaaa
    58561 aaatatgttc gacaatgtaa aaatgggaaa aatagtttaa ccgtacaaaa aatataatgc
    58621 tatactctaa ctatagtaaa aatagtaaag gagtaaaatt tatatgataa ttggatatga
    58681 aagagtaagt actaaaaaac aaaacaatag tagacaagaa agagttctag aagaagaagg
    58741 aataaaattc aataaaatat atagagataa aacaacagga gcaactaaag atagaccagc
    58801 attaaatgaa atgttattga atgtgaaaaa aggagatacg gtatactgtg aatcaattag
    58861 tagactagga agaaacttaa aagatttgat taatataata gatactttaa ttgctaaagg
    58921 ggttagagta aaaatattaa aagaaggtat agacacagga aacaatactt ataaactatt
    58981 acttggtata tttggagctg tagcagagat ggagagggaa acaatacaag agagaacaca
    59041 gcagaaaata gaccagctaa aagaggataa gaaaaaaggg gagataaata caaaaacagg
    59101 taaatggttc ggtagacaaa aaataactaa agaagattta gaaaacaaga aagaatttgt
    59161 taaatattat tcaaaaatca aagataaaag catgaataaa attgaagtag ctaaaatttt
    59221 aggaataggt agaacaactt tatatgagtg gatattaata tatgaaggtg gacaagtaaa
    59281 aagaaaaagg aaaaaaagtt agtattctat gagttaattg atgtaatcaa atatggaggt
    59341 atttttatga gttttgataa gaaaagatta atatgtgcag gagatttttt aattgaatta
    59401 gacgaagcaa ttgcagatat aataatagaa cttaataaaa aaggttataa aacattgaat
    59461 tgttgtagtg gacatattaa taatgatggt gaaactaatt gttatatatc tttctatgaa
    59521 tcatataatt ttacccttcc aaaaaattat tgttatgaat cggataattt aattagatgt
    59581 aacttttatg gaaataaatt agaattaaaa aagcaactaa accaaaatat agagatattg
    59641 agaaaatggg ttaatggatt acagacatat aatcaagtta ttggagaaat tagaagtaga
    59701 aatgagaata aaaaacattc ttacattaaa tgtttgaata aggatgtgag aataattaaa
    59761 tcagagataa gtgaaattgt cttaatggaa atagataaat tggatattaa tagtttgaaa
    59821 gttattttaa aagaaattgg aataaaaaat tatagtaaat taaaaaagaa tgaattactt
    59881 attatactaa atgaaaattt ggatatttta tctaatgaag atagaaagga agttctatat
    59941 tggatatatg aaaataacaa aataaaattt ggtatgtttc agaaagaagt tattgagtac
    60001 ttgaatattt caaaaggaga atttaataaa gtaagtaatg agttaaaaga ctttgaaatc
    60061 ggaatagaga aacttaggat tgggggtaaa atgcagtctt acataaagta tgatagaatg
    60121 aaaatttata atatatttta taaagctcta taagctgtat atagagcttt atatttaaaa
    60181 aattaattta tttcttaaaa taatcaatta aatcgaataa agttgataaa ataagtttta
    60241 gtttaaatag attatattct ttctttctat aatctgtatt ttttttattt gatttttttt
    60301 tgttcatacc attacccctt ttctttttgt gatttttaat tgattttttc atatgtaatt
    60361 acctccttta taagtaatat aaaaatgttt ttaagaagta t
//
LOCUS       DAYASV010000008        58964 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYASV010000008 DAYASV010000000
VERSION     DAYASV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58964
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.008"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(124..2253)
                     /locus_tag="ACGI68_000554"
     CDS             complement(124..2253)
                     /locus_tag="ACGI68_000554"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFH0137012.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            2477..2767
                     /locus_tag="ACGI68_000555"
     CDS             2477..2767
                     /locus_tag="ACGI68_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137013.1"
                     /translation="MSGLKKILRILVISMMICFAMTFALVLINSVMGVHIGLDYYFLM
                     GSVLTVCLTIGIVLCNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            2933..4324
                     /locus_tag="ACGI68_000556"
     CDS             2933..4324
                     /locus_tag="ACGI68_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFH0137014.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            4439..5908
                     /locus_tag="ACGI68_000557"
     CDS             4439..5908
                     /locus_tag="ACGI68_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFH0137015.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            5973..6566
                     /locus_tag="ACGI68_000558"
     CDS             5973..6566
                     /locus_tag="ACGI68_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137016.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            6697..8094
                     /locus_tag="ACGI68_000559"
     CDS             6697..8094
                     /locus_tag="ACGI68_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0137017.1"
                     /translation="MEVSIILVKDIKQDIESMPRKILDISLIEDNKSDLTFFNIEEED
                     GDETLKALKQSYVTTNYKEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKN
                     YITSAINMVNKALEINPKDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKY
                     VNLISSEDFKTFLGRYNHSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDK
                     LGNVKKREGYLDKLKELDKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCV
                     LIGMGIYLIQSKKRIENLNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEP
                     EREITVADEEDLYYQALNLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKL
                     LGNKDEAIKYYKKYINTYTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPD
                     SMYNNSKIKEILSEK"
     gene            8276..8647
                     /locus_tag="ACGI68_000560"
     CDS             8276..8647
                     /locus_tag="ACGI68_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFH0137018.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            8640..9329
                     /locus_tag="ACGI68_000561"
     CDS             8640..9329
                     /locus_tag="ACGI68_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFH0137019.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            9849..10715
                     /locus_tag="ACGI68_000562"
     CDS             9849..10715
                     /locus_tag="ACGI68_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0137020.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            10957..11766
                     /locus_tag="ACGI68_000563"
     CDS             10957..11766
                     /locus_tag="ACGI68_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFH0137021.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            11910..12974
                     /locus_tag="ACGI68_000564"
     CDS             11910..12974
                     /locus_tag="ACGI68_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFH0137022.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            13002..13721
                     /locus_tag="ACGI68_000565"
     CDS             13002..13721
                     /locus_tag="ACGI68_000565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0137023.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            13745..14329
                     /locus_tag="ACGI68_000566"
     CDS             13745..14329
                     /locus_tag="ACGI68_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFH0137024.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            14331..15680
                     /gene="pabB"
                     /locus_tag="ACGI68_000567"
     CDS             14331..15680
                     /gene="pabB"
                     /locus_tag="ACGI68_000567"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HFH0137025.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            15670..16413
                     /locus_tag="ACGI68_000568"
     CDS             15670..16413
                     /locus_tag="ACGI68_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HFH0137026.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDIKYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FVKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            16447..17148
                     /locus_tag="ACGI68_000569"
     CDS             16447..17148
                     /locus_tag="ACGI68_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137027.1"
                     /translation="MIGCIYEVYNEEITKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRNIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            17174..17746
                     /gene="folE"
                     /locus_tag="ACGI68_000570"
     CDS             17174..17746
                     /gene="folE"
                     /locus_tag="ACGI68_000570"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFH0137028.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            17810..18601
                     /gene="folP"
                     /locus_tag="ACGI68_000571"
     CDS             17810..18601
                     /gene="folP"
                     /locus_tag="ACGI68_000571"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFH0137029.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            18612..18977
                     /gene="folB"
                     /locus_tag="ACGI68_000572"
     CDS             18612..18977
                     /gene="folB"
                     /locus_tag="ACGI68_000572"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFH0137030.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            18970..19476
                     /gene="folK"
                     /locus_tag="ACGI68_000573"
     CDS             18970..19476
                     /gene="folK"
                     /locus_tag="ACGI68_000573"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFH0137031.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            19457..20242
                     /gene="aroF"
                     /locus_tag="ACGI68_000574"
     CDS             19457..20242
                     /gene="aroF"
                     /locus_tag="ACGI68_000574"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFH0137032.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            20497..22287
                     /gene="dnaG"
                     /locus_tag="ACGI68_000575"
     CDS             20497..22287
                     /gene="dnaG"
                     /locus_tag="ACGI68_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFH0137033.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            22305..23471
                     /gene="rpoD"
                     /locus_tag="ACGI68_000576"
     CDS             22305..23471
                     /gene="rpoD"
                     /locus_tag="ACGI68_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFH0137034.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            23636..24325
                     /locus_tag="ACGI68_000577"
     CDS             23636..24325
                     /locus_tag="ACGI68_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFH0137035.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            24339..25436
                     /locus_tag="ACGI68_000578"
     CDS             24339..25436
                     /locus_tag="ACGI68_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFH0137036.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSTEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            25499..26071
                     /locus_tag="ACGI68_000579"
     CDS             25499..26071
                     /locus_tag="ACGI68_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0137037.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(26131..26598)
                     /locus_tag="ACGI68_000580"
     CDS             complement(26131..26598)
                     /locus_tag="ACGI68_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0137038.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(26984..27460)
                     /locus_tag="ACGI68_000581"
     CDS             complement(26984..27460)
                     /locus_tag="ACGI68_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0137039.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(27580..28671)
                     /locus_tag="ACGI68_000582"
     CDS             complement(27580..28671)
                     /locus_tag="ACGI68_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HFH0137040.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFIPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYAKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLLTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(28664..29053)
                     /locus_tag="ACGI68_000583"
     CDS             complement(28664..29053)
                     /locus_tag="ACGI68_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0137041.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            29245..29691
                     /locus_tag="ACGI68_000584"
     CDS             29245..29691
                     /locus_tag="ACGI68_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137042.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEELDLHEDAKKDNIYKEDEYKKYEEE"
     gene            29955..30680
                     /locus_tag="ACGI68_000585"
     CDS             29955..30680
                     /locus_tag="ACGI68_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137043.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            30658..31911
                     /locus_tag="ACGI68_000586"
     CDS             30658..31911
                     /locus_tag="ACGI68_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0137044.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMV
                     VRNNQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPI
                     ISKLVLETIKINIILVCIIFILIFVLIVRYFTSIYNDLSDMTKYVYYSSEGKPFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMLSMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITY
                     KENPLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            31991..32668
                     /locus_tag="ACGI68_000587"
     CDS             31991..32668
                     /locus_tag="ACGI68_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137045.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQVSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            32669..35230
                     /locus_tag="ACGI68_000588"
     CDS             32669..35230
                     /locus_tag="ACGI68_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137046.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVKNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINSSI
                     KSIIIAVTILVIVCTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIIIIVSIIIVLLTIF
                     ISILLPAISASNISPLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAIEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNIRRESI"
     gene            35451..36725
                     /locus_tag="ACGI68_000589"
     CDS             35451..36725
                     /locus_tag="ACGI68_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HFH0137047.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(36886..39927)
                     /locus_tag="ACGI68_000590"
     CDS             complement(36886..39927)
                     /locus_tag="ACGI68_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HFH0137048.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKIDDNNISNSVKEAWKKRNGKDYYLVDEKYTSQSYMFGSVKATLALSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            40133..40432
                     /locus_tag="ACGI68_000591"
     CDS             40133..40432
                     /locus_tag="ACGI68_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFH0137049.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            complement(40671..42899)
                     /locus_tag="ACGI68_000592"
     CDS             complement(40671..42899)
                     /locus_tag="ACGI68_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFH0137050.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            43192..44967
                     /locus_tag="ACGI68_000593"
     CDS             43192..44967
                     /locus_tag="ACGI68_000593"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137051.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
     gene            44980..46803
                     /locus_tag="ACGI68_000594"
     CDS             44980..46803
                     /locus_tag="ACGI68_000594"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137052.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRILNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSILLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            46921..47466
                     /locus_tag="ACGI68_000595"
     CDS             46921..47466
                     /locus_tag="ACGI68_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFH0137053.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            47726..48334
                     /locus_tag="ACGI68_000596"
     CDS             47726..48334
                     /locus_tag="ACGI68_000596"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFH0137054.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            48661..51558
                     /locus_tag="ACGI68_000597"
     CDS             48661..51558
                     /locus_tag="ACGI68_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0137055.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLSSGYEYSALYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNTAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKDKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDEESNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMLCGSINENDKVKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSVESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLRKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEEFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            51879..52091
                     /locus_tag="ACGI68_000598"
     CDS             51879..52091
                     /locus_tag="ACGI68_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0137056.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            52104..52325
                     /locus_tag="ACGI68_000599"
     CDS             52104..52325
                     /locus_tag="ACGI68_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0137057.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            52425..54581
                     /gene="feoB"
                     /locus_tag="ACGI68_000600"
     CDS             52425..54581
                     /gene="feoB"
                     /locus_tag="ACGI68_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0137058.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            54607..54759
                     /locus_tag="ACGI68_000601"
     CDS             54607..54759
                     /locus_tag="ACGI68_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFH0137059.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            54939..55649
                     /locus_tag="ACGI68_000602"
     CDS             54939..55649
                     /locus_tag="ACGI68_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFH0137060.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            55734..56504
                     /locus_tag="ACGI68_000603"
     CDS             55734..56504
                     /locus_tag="ACGI68_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137061.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGANKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            56497..57228
                     /locus_tag="ACGI68_000604"
     CDS             56497..57228
                     /locus_tag="ACGI68_000604"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137062.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            57262..58221
                     /locus_tag="ACGI68_000605"
     CDS             57262..58221
                     /locus_tag="ACGI68_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0137063.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(58319..58915)
                     /locus_tag="ACGI68_000606"
     CDS             complement(58319..58915)
                     /locus_tag="ACGI68_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HFH0137064.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT        23313 a         6290 c        10004 g        19357 t
ORIGIN      
        1 attgtattat gaaaaaataa gtatctaatt tataaacaat taataatagt attaattaga
       61 tacaacttat ttaatataat aaaatctgct ttttatataa ctataaaatt ttactacatt
      121 ctattagaat tgcccataaa taccttcaag ttcttctttt aatacttcag ggtcatttat
      181 accaatagac atggttctag ccagtatatt tgcttggtca taagttgatg tccatgagaa
      241 tacaccacct agacaatttc taagaacata ttgagttttt aagaatattg ataaagcatc
      301 atcatatgac attgcaaaat ctccatcctt aactaaatac ggaacttgag cagtattatc
      361 aaatctatat tcatatccat ttttatttat ataatccctt ctaagctcat cataagttct
      421 tgttatagta gcacctaatc ttccataaaa tggtatacca agaaggattt tttcagaagg
      481 cattccagca ttctcaagat ttcttaccat tgcatcaaca ctatatcctg gcaaagataa
      541 gtctgaatca aaaagatttg cttgatgttt tctaccattt gggcctgttt cacctgctgt
      601 aaaatcataa ctcataagat taaaataatc tattatagga gctattttat ctatttcagc
      661 acttgaattt atatatcctc tatctcctgt tccagctaca ctaagccatt tatcatctcc
      721 tataacatct cttatggcag ttagtaagag tgtaaagttt tctctatctt gaggtcttga
      781 tgttattcca gatgcactac ttccaggata ttcccagtct atatctattc catctaaagc
      841 atattcattt atcatctgat tgacctgtct tgcaaaatta tatctagatg taggtgttaa
      901 tgctgcatca gagaatcctt cagctcccca acctccaata gctacaatta cttttaagct
      961 aggtttttca cctttaagag caagtaattg atttaaaaat ctaggataag gaataaacaa
     1021 atttccagac tggtctatct cagcaaaagc aaatataact gcatctaata gttgagcatc
     1081 tacattagtt ggatttccaa gaacatattc tgctacaatt ggatttatat ctggacagtt
     1141 tgcattttca actattggat tgtatgggtc tgttatttgt ggtctaacta attttttaga
     1201 atcttttaaa gttttcatag ctattttttt agtatttttt tcgctagtat aatcgtagtt
     1261 tttataatta tgtttataga aatcttcata actactatct tctttgttat agctagaatt
     1321 tattttgtca caagattcat ctttatcata gttaaaattc atttcatcac aagacttatc
     1381 tttaaactta tcttgtttgg agttgtgaac tttgttagta tttttagtat gttttttctc
     1441 atgattgcaa tcatctttaa tatcagtctt gtgttcttgt ttacagtcaa gagctttgtt
     1501 caggtagtca tgatgttcat gttcacagtt acattcattt tcaatgtttt tagtatgttc
     1561 ttttttccag ctacaagtat tatcaatttt cttgctcact tcttcatcat tataattatc
     1621 tggtacaaaa cataagtacc agtccataca tgaatatttc ttattacaac tgttaactct
     1681 attttttaat tccttatagt ttttaggata aggtaaaatc actggcattc caggaaacca
     1741 gtttgcagga gtaactatat tatctctatc actagtctga agtgcatcta ctattctaag
     1801 tatttctgga atatttcttc ctgtagttag tggataataa agaatcgttc ttagaatttg
     1861 attattatct ataataaata cagagcgaac agttgatgta tcactcattg gttttgatat
     1921 catgccgtat aacttggcaa ttctcatatc tctatcttct ataataggaa atggaatttc
     1981 tacacctgta agtaaagaaa tattatacat ccaagctaaa tgtgaactgt tactatcaac
     2041 acttagacca attagttctg tatttctttt tttaaattcg tcataatatt tagcaaaaca
     2101 taaaaattct gtagtacaaa ctggtgtaaa atcaccagga tgtgaaaata aaacaatcca
     2161 attaccctta tagtcagaga gtctaatagg accatttgtt gtattggctt taaaatcagg
     2221 agcctttgac cctaaacttg gcaaatttgg catgtaaatc acccctcaat aaattatcat
     2281 ttattttatt ctattaatta atacatatta gtgttactat tttattaaaa gttaagtaat
     2341 ccttatgtca aaaaaagata tttattaaaa agatttaata ttttcctaat ataaatgaca
     2401 atagtaattg ctattttttt actaatttgg tatagtaatg gataaggggt agtgtcaatt
     2461 ttagtgatta tacgaggtga gtggattgaa aaaaattcta agaattttag ttatctccat
     2521 gatgatatgt tttgcaatga cttttgcatt agtgttaata aattcagtta tgggagtaca
     2581 tataggattg gattattact ttttgatggg aagtgtgctt acagtttgtt taacaattgg
     2641 aattgtttta tgcaatgcta gaaaaatatt tgatagcaaa gctatacaca agacaagaac
     2701 gaaaactgtc agtaataata aaaaacaaaa gagtcattct agggatataa aaagaaagat
     2761 atcataattt atgtaaagga aatatttatg tataaaataa tgattgatag gtaaaataaa
     2821 aacacgattt aacttgtgtt tttatttttt ttcaagaatt atgatataat aaccaataag
     2881 aaatttagtt taccataaaa tataaagatt attaaatgga gagggaacta taatgaattt
     2941 tagagattta tttgagaagg ctgtagattt tattgatgaa aaaaggaaat ctatagttgc
     3001 gatttcatta tcaacattag gtgtaattgc tttgataata gtatttttct taagtagtaa
     3061 cgagtttagt gtaggaaatg aagctaatga gttgttaaag attatagaga aaagacagta
     3121 ttctatagct gttgattact acacaagtat agaaaaaaag ttttctgatt caaaaatgga
     3181 aaggtttaat aagtctgttt ctaaaaaaat aaataaattg ctattaaata gtggtgataa
     3241 atatttagat ggagacataa gtcaagaaag ttttattgga ttaataaata ccgtgaaaga
     3301 attaaacaaa ataagtatag atgtggatga cttattagct caagctgata gagttagaga
     3361 aatgtataaa gaagaaaata ctacttatga tattgccatt aactacataa gtactgtttc
     3421 aatattaaat ggtatgggta gtaacctaga tgtatacaaa cagaatatag aaacaatcaa
     3481 agagtctaga gatgtttatg actcagcagt aaaagaccaa aaagcatata agtattatga
     3541 agctattgaa aagtataata aagtattaaa agaagatgaa aagtattaca gtatggctca
     3601 aaatgggaaa gaacaatgta ttgaagaaat gtatgattac tatataagta gagctgaaga
     3661 ggcaaatact tctggagatt atgaaagagc attacaatat attgagtatt taaaagacga
     3721 ttattcagat gatgaaaagg tacagagttt agaaaaaaaa tataaaaaga atttatcatt
     3781 gtatacttta acatctgatg acattataaa tgtaatagct aaaaaaagtg gcaaagacaa
     3841 ggcaaatcta tctataaatt cacttccaca aatgataaaa aataataaat actattatgc
     3901 tgaagtatat gagtatgata aattaataaa tgaagttttg gtaagtgcaa aaactaaaga
     3961 tttatattca tataaagatg gtaaaaaaga ttacaaagta gattatggtg atggatactt
     4021 tagaatatta gaagatggaa gttatcaatt tggtattaca aaagataaag ctaagtttgt
     4081 gttaactaat actcttgatg aaaaagaaaa taagtataaa aagattgaga tattagatat
     4141 agagaaagca gatagatatg taaaaagtaa aaagagttta gaagaattgt ttggaaaata
     4201 taaaaatatt tattattatg ctgtagtaaa taaaggctta tttagaggca aagaagttta
     4261 tgctattaat atttacaatg aaaaaatata tgccatatca gaaaatggac taaatgaata
     4321 ttaattaata atattaaatt aatatataat taaaaagttt ttggggtttt aaggcgcatt
     4381 atgtagtaat ataataatag ggatatataa ataattttat tgtaaggaga agtatgggat
     4441 gagtaatgtg aacaaaaagc tagttatagc tattggagct gtagcattag tagtaataat
     4501 ttttataggc ataatgtgca tgcaatttaa aggggagaaa atagcaaaaa acacatatgt
     4561 taatggagta aatattggaa aattaactaa atcacaagca aaacaggaat tagctaaaaa
     4621 atacaaatta gagaatgttg attttaatta caatgataaa agctggaaag taaagagcaa
     4681 agatttaaat ttatctactg acctggataa aacagtagaa aacgcatata atttaaatag
     4741 aaagagtgct ttctttggca atctatcaaa aactatttca gcaaattttg gcaaaaaaag
     4801 caacttagta gttgcgataa attatgataa aaataaatta aaagcagaaa tggaaaagat
     4861 agctaaagaa atagatgtag atgtaaaaga tgctacactt gatataagtg gtgaaaaagt
     4921 caaggttata ccagattctg atggtcttaa aatggatatt tctaagagta tggagaattt
     4981 tgataatcaa actaaaaaag gtaactataa aaatgagtta gtagtaaaag ctactccagc
     5041 aaaagtaaaa aaagagcaac ttgctaatat agatacaaat ttaggtacgt attcaacaac
     5101 atttaaaact tctcaaataa atagaagtat aaacataaaa ttagcaacag ataatataag
     5161 taatgtatta cttatgccag gagaaacttt ctcatttaat aaacatacag ggaaaagaag
     5221 taaggaaaat ggatataaaa gtgcacctgt aataatggaa ggtgagatgg aagaagatta
     5281 tggtggagga gtttgtcaag tttcatctac gttatacaat tctgtgctat acgcaggttt
     5341 agaaattgtt aatgtaaaaa atcatactat accatcaagt tatgttccaa aaggaagaga
     5401 tgcaactgtt gcagatagtg gaatagattt cttatttaaa aataatctaa agcatccagt
     5461 ttacataaaa aattacgttt caggaaatca aattgtttgc aatatatatg gaagtgcaga
     5521 agataaacaa aatataacaa tatcaacaaa attagatggc gtttcacaaa ctacaatgaa
     5581 aagagttaat gaccctacaa tgcctaaagg taaagaaaaa gtagacaaaa gtggaagaaa
     5641 tgcatattct gtatcaacat atagaacatt taatgatgca aatggtaaaa aaattaaaac
     5701 tgaaaaaata gctaattcat attatcctaa gaaggaaggt attatattag ttgggactat
     5761 ggaaccaaaa ccagaggaaa aaccaaatac agacgaaaat aagaataatc aaaatacaaa
     5821 caatcaaaac ccaaataatc aacaaaaacc agaaacacca ccaactgata ataagcctaa
     5881 tgagacacaa ccacaaccgc aagcttaaag ttctcaagaa taggatttat gtagagtaat
     5941 aaaaaatata aatctaaata ggagggatta ctttgaattt aaataatata acgaattacg
     6001 ttcaaagtat ttgcgagaat atttctaatg tcttggatgt agatgttact ttagttacaa
     6061 aagatttaac taggatagca ggtacaggaa tattcagagg taaaatagga gagacaatat
     6121 cagagaagtc agtgtactct cttgttttaa aagatgggaa atcctatgtt ataaataaag
     6181 aaatagaaga gaactgtaat aaatgtgcaa atagagaaaa ttgtaaagag ttagctgata
     6241 tatgtactcc cgttaaaatg ggagagcata tacttggaat cttgggtata gctgcgttta
     6301 atgaagaaca aaaagaaaag atactttcaa aagataagga gttaactgct tttgtaagga
     6361 gtatgtctga tttaatatct tttaaactag atgaacttta taaacaagaa gttaagacta
     6421 tagatgacct tgagagggaa gctatagaaa atgccataaa gaaatatgga agtaatacag
     6481 aaggtatgaa aaaagtagct gaggcattaa atataggtat agctacactc tatagaaaag
     6541 taaaaaaatt caacattaaa agttaatagt attgaaaaag aggtaacctt tttatgaaaa
     6601 gaagaggatt acctttttta ttatgagaat ggtggaaaaa tatgtatagt tttgacttga
     6661 catattaaat atgttatctt aaatatatat tttagagtgg aggtgagtat tattttagtt
     6721 aaagatataa aacaagacat agaaagcatg cctagaaaaa ttttagatat atctttgata
     6781 gaagataata aaagtgattt gacttttttt aatatagaag aagaagacgg tgatgaaaca
     6841 ttaaaagcat taaaacagag ttatgtgact acaaattata aagaagaaac tctattaaac
     6901 ttgcaaggag ctttaaattc aaagtcagat acaacgtcta aggagttaaa attagaatca
     6961 tataaaagat ataacaatgc attggattta gcatataaaa attatataac tagtgccatt
     7021 aatatggtta ataaagctct ggagataaat cctaaagaca ttgatatact aaatcttaga
     7081 ggtcttttaa aattattaaa atgtgatttt gctaaatcat ttgagagttt ttatactgct
     7141 ttatgttatg aaaataatca tatatctaga aaatatgtaa atctaatttc atctgaagat
     7201 tttaaaactt ttttaggaag atacaatcat tctataagat ttataaatga agaattaaat
     7261 tatgagtcaa tacatatttt agagaacata attgaagaag aacctgaact aatagagcca
     7321 tatgtaattt tatctttgtt atatgataaa ctaggtaatg taaaaaagag agaaggatat
     7381 ttagacaaac ttaaagagct agataaagat aatcctgtat ttgaaaaaaa tgaagaagaa
     7441 aaagaagata caagtaaaaa ggaagaaata aaaaaagtaa aaagaaagaa aagtattttg
     7501 ccatatatta tagttggctg tgttttaata ggtatgggga tatatttgat tcaaagcaaa
     7561 aagagaatag aaaacttgaa taaccaaata agtagtaaag aggaaaagtt aagtgaaact
     7621 gataaaaagt taggagaaac gagtaaaaaa ttagaaaaaa cgaataagga attaaatgag
     7681 gttaaaaata aagaaccaga aagagagata actgtagctg atgaggaaga tttatattat
     7741 caagctctaa atttaaagaa aaacaaagaa tatgaaaaag ccatagataa ttttaaaagt
     7801 gtagtttctt ctggtaaaac taaaaaatat atttctgagt caatatatca attagcaata
     7861 acaaataaat tattaggaaa taaggatgaa gctataaaat attacaaaaa atatataaat
     7921 acttatacta aaaatgacca gtattatgat gattcatatt acgagttggg tatgctttat
     7981 tatgataatg gcgatttaaa aaatgcacaa cagacttttt atagtttgag aagtgaagtt
     8041 ccagacagta tgtataacaa ttcaaaaata aaagaaattt taagtgaaaa ataggtttat
     8101 atagttaagt tttttgaaac aaccaagtat gtattttatt aaattaaaat aaaaataaat
     8161 ttaaaaagag attgtgtcaa attataactt tctattttaa agaaagatat ttgcttgttg
     8221 ataaaactac aaatatatga tttgaagcaa tctctttaat ttttggaggt ggaaaatgaa
     8281 agttttcaat caattggtaa taattttatc tatctgggta ataggagagt atataagttc
     8341 ctttatacaa ggaattatac ttattcctgg gagtatagtt ggtatgctat tattatttct
     8401 actactacaa tttaaagttt tagacttgag tagtatagaa aatgtaagtg gatttttcct
     8461 cgataatatg gctatatttt ttattccagc aggtgtatca cttataaagt cattagacct
     8521 aataagggaa aatgtattag tgcttttatt agtcatatgt ttaagtacac taatagtaat
     8581 gtatactact ggaattattg tggagaaaat gataaaaaag aaggaagaag gacaaaaaaa
     8641 tgtataatgt attacagaca ccagtatttg gtataatagt aacaatagtt tttttcaatt
     8701 taggaagata cattcaacga aaaacaagta atcctatatg taacccttta ttaattgcaa
     8761 tcgtaggaat aatattgttt ttaagcatat ctaaaatccc ttatgagaat tacaagatag
     8821 gagctgatag tctaaatttc tttttaggtc ctgtcacagt agtacttgca gttccacttt
     8881 ataaacaatt tgaattattt aaaaagcata tgtttgagat tattgtagga ataggttgtg
     8941 gtatagtgat ttcttttgta tctgtattga taattggcaa aatagctaat tcagatgtaa
     9001 gtataataaa ttcactaatt ccaaaatcta taacaacacc aatgggaata tctttagcaa
     9061 attctttaaa tggtgtagaa tcaataacag ttgtagccat aatttttaca ggtatttttg
     9121 gtggtatggt tgcttctaca gtattcaaac tggggaatat aaatcatcca gttgctaaag
     9181 gaattgctct tggaacatct gcacatgctt taggaactac aaaggctttt gaacttggag
     9241 aaatagaagg agctatgagt ggtgtttcta ttggagtctc tggtacaatt acagtaatac
     9301 taattccaat tataatgaat tttatataaa aaataaaatt aagacgagta acttgaatac
     9361 ttaaaacaaa ataaaataaa aaaaatttaa aataaaaact gaacgaaaaa aatgcaaata
     9421 atgaaataaa aaaatcatgt actgaaatta taaaaagaaa aatttcattt aggaattaat
     9481 tataaagaga caattactaa acttgaattt gctaaaataa aataaattac tgcgaaaata
     9541 gatatttaga aaaaaatact ataaatttac tatattgcaa gttaaataat aagcagtatc
     9601 atattatgct agaaaaagtt tcatttaagg cataaatgag atgatttgaa cgtttttaaa
     9661 gattagaata taaaagattg cactagacat atatgaaaaa gataatataa tagatgatgt
     9721 aatagatgac agttcaagaa attataaaaa aaatttattt gaaatattat ctattacatc
     9781 aggaataata tatgtaagag taagctttta aaattctaaa gatttagaaa gattgaaggg
     9841 agaaagaaat gagtgaagtt aagaataccc tacaaaaaca tccagtttta agatattcaa
     9901 caatgctatt tgggatgttg atgacatcta taggaataaa tgggttctta agaccagcac
     9961 atctacttag tggaggtgct acaggtatag caacatctat aaactatttg acaaatataa
    10021 atgtaggttt attaacattt ttaatcaata ttccaatatt tatcttaggg ttcatatacc
    10081 ttgaaaaaga gttttgtatt tcaagtttgg taaatatgat tgtattttca ttattattag
    10141 gagcaactca ggaaataagt aatattatac ctatacatga tatattgctt caaagtgtat
    10201 atggtggtat attatctggt ttaggagtcg gagttgtatt tagaactagg tcttctcaag
    10261 gtggtacaga tataatagct gcaatactaa aaattaagaa gaatatagaa atgaaagaca
    10321 ctgctttagc aataaatggc ttgatagtac ttacaggaag ttttttattt gggttggatt
    10381 tagcattata cactcttata ggtctatttc taaatgcgta ttcaatgagt tttattaaag
    10441 atgcaatgaa ttaccaaaaa tctgttatgg taatgtcaaa tgaagtcgat ttaatagcag
    10501 aagatataat gaagagttta gttagaggtg taactttttt agatgcagaa ggtgcataca
    10561 ctcatcaaaa aaagaagatt atatatacaa tagtttcatc aaatgagata ccaaagataa
    10621 aagatatagc tttgaagtat gataaaaagg cctttatatc tgtaaatgat gttacagaag
    10681 taaaaggaag agggtttaaa gcaaaagatt tatagaaaat tttacaagct gggagtttat
    10741 aatgtaaatt tgtatattat atctctcagc ttatttaaat ttagtatacg aaaataaagg
    10801 ttttattaat gatttgtgta tcactttaaa agatatcatt ttaaaattaa aattgaaaaa
    10861 attacttaat atgtagaaaa catgaccact attaggatat aataaattta atagatttag
    10921 taaaatatct aggaataacg ttggaggaat ggcattatga atttatctaa agaatatgtt
    10981 aatttgtatg ataattattt gaaatatata aatgaagtaa acaaagagat tagaagtata
    11041 aagaacatga agaaacgtgt tttaaaaagg ggcgattttg ctccagaaaa tgaagtatta
    11101 gaattggaag gaaaaacaat acgcgatata gatgatgtcg atacaaaaaa accaaaaaat
    11161 aaaatctata aattttcaaa tggggatgta tatgtaggga aattcttaga gggaaagatg
    11221 gaggggaaag gctcatatac tttttttgta gatgaaggaa cagttatgga gtacatagga
    11281 gaatttaaag aagataaaaa aaatggaaaa ggaaacttta tattttcaaa tggaaatgaa
    11341 tatataggtt attttgagga agatatgatg agcggtattg gaaatatgtt atatagtagt
    11401 aaagatgaat atattggaac ttggaaaaac ggaaaaaaag atggaaaagg tatttataag
    11461 tggagtgatg gatgtatata tgcaggtgaa tttaaaggtg gaaagatgga agggtacggt
    11521 atttgctata atagtaaagg ggaagtactt tatgaaggag aatggaagaa taatttgatt
    11581 catggtaagg gaacttatat atgggaaaaa ggtaaaaagt atataggtga atttatgcat
    11641 ggtaaaaaac atgggcaagg aactttttat ctaaataatg aattggttta tgaagggact
    11701 tggaaatttg ataagccatc tatatttgat agaacattag atgaaatatt ttccttgaga
    11761 ctgtagcttt atctaattag tagataaagc ttttttctaa tacttactgt aaataggtat
    11821 gaatgatatt gattagtata cactatcaat attaacttaa taaatgcaat attaaaaaat
    11881 tttgattgat tttaagaagg agagatatat tgaagagatt aattgcttta gtgttagtag
    11941 tacttattgt tttattttat agtcctgaat tattaaaaga aggatatgat ttaaaagaaa
    12001 agttttttca aagtgaaata tggaatgaat ttttagacaa aataaatata aatgataatt
    12061 ctaagaaaga taaaaaagaa aaaagtcaaa atttacaatc aaataatact gaaggggaga
    12121 acttaggaga gtcagatata aagaattttg aaaaaataag cttaggagat aatttaagct
    12181 atgttttatc aagtatcggc aaacctggta gaattgatat tagtgaatat ggttttgatt
    12241 ggtatgtata taatcaatat ggaaaagaat ttgcaatggt tggacttgaa aatgatgaag
    12301 tagtagcact ttatagtaat tctattaatt catgtgaaaa tcaagatata aaactaaatc
    12361 aagatagaca aacagtgaga actaagatta caccattaaa atataagaga aaaggaaata
    12421 ccagatatat aataaactca gaaaatcaat atgatataat aagtaaggaa ggtaagtata
    12481 ttacaatttt ctacgatatt catgaagaaa atagagtttg ttcatattta ataattgata
    12541 aaagcactga agatgagttt aaaaatttat atccagatga tagcgaagaa cttaaaaaat
    12601 gttttgaact tgaagttata gaccttgtaa atagtgttag aaatcaaaga gggttaaact
    12661 cattgagata cagtgaacag gcaactttga gttcaagaaa acatagtgaa gatatgagag
    12721 ataataattt ttttgaccat gtaaataaga agaatgaaac tccatttgac agaatgaaaa
    12781 gagaaggtat agtatatact tcagctggag aaaacatagc agcaggacag attaatgcaa
    12841 tatatgcgca tgaagcttgg atgaactctg aaggacatag aaaaaatatt ttggggaact
    12901 ataataatat tggagttgga gtaatatttg gaggaagcta taaaacatat tacactcaaa
    12961 atttttataa ataactaaaa tatttgaaag gaagttaaaa aatgacatca tggaaaaaaa
    13021 agacagtata taagtgctta atagcagtag tcttgttttg tggaatagtg ttaatatcta
    13081 attttagtaa agtttctgct ttgatgatgg atactaatgg aaatgttttg attaaacatg
    13141 gttcaaggga aaaaaagcta atagcaatta cctttgatga tggtcctcat cctaaagaaa
    13201 ctagtcaagt tttagatgta ttaaagaaat acaatgtaaa agctactttt tttatagctg
    13261 gaaaacatgc aaaatggtat aaagaaccat tagtaagagc aagtaaagaa ggtcacgaga
    13321 taggaaatca tactttcaat catcctgata taagtaattt aagttctagt cagatagaag
    13381 aggagattgt taagtgtgaa gacattttga aagaagtaac tggaaaaaaa ccgacattat
    13441 ttagaccacc atttggaagt tatagagaaa aagaattaat agaaattgca aaaaagcatg
    13501 actataaagt tgttttatgg acaggtgtag atgtgaaaga ttggaaaaat ccaggagcta
    13561 atagcattgc agataaaata attaataaag tacaaaatgg agatataata cttttgcatg
    13621 attatgcaac taatgataca gttgaagctt tagatatgtt tattccaaaa atgattgaaa
    13681 aaggttttaa atttgtaaca gtttctgagt taataaaata atttttaggg gggagattta
    13741 cagtatgatt cttatgatag acaattatga ttcttttgta tacaacctag tccaatatat
    13801 agaagaatta ggggaaacag ttgtagtaaa aagaaataat gaaatcaaaa taagtgacat
    13861 tgaagaacta aatccagagg taattgtgtt atctccagga ccgtgttctc caaaagaagc
    13921 tggtatttgc atagatattg ttgagcattt taaggggaaa aaacctatac taggaatttg
    13981 tttaggtcac caaactattg gacatgtttt tggaggagat attataaaag cacaacagcc
    14041 tgtgcatgga aaagtatata gtatcaacca tactaataaa ggagttttta ggggacttaa
    14101 aaatccttta aatgtcacta ggtatcattc actaatagtt gactccaata cagttccaaa
    14161 agaattagaa attacagcaa ttactgataa aggggaaata atggggatta ggcataagaa
    14221 atatttaata gaaggagtac agtttcatcc agaagctatt ttgtcagagt atggtcatga
    14281 aatgcttaaa aattttataa cagaagctag agaaagggta catatgtaat atgataagag
    14341 agataaatac taaattaaat tcttttgaga tatttactat ttttagaaat gaacatgaca
    14401 gttttatatt agacagtgct atggacaaag aaaaattggg aagatattct tttataagta
    14461 gccaaccatt taaggtatta aagtataaag atactgatga aaatccttta gaagttttaa
    14521 aagaagaact acataagtat agggttgtaa atgatactaa tcttccattt gtaggaggtg
    14581 ctgtgggata tttatcttat gatttaggta attacataga aaatttacct agaactgctg
    14641 tagatgatat tgagatgcct gatatgtatt ttggatttta taatcatgta atagttatag
    14701 accatcttgt gcaaaaaact tatatagcta ctccaaacat agatatcgaa ttagaagaaa
    14761 agataataga tgatatagaa caaagaatat taaaagaaga gaaaaaaggt atagatagca
    14821 tatgttatga agaaaaagaa gtaacaccta taagacttaa atctaatttt acaaaggaag
    14881 aatttaaaaa tgcagttcaa agtgttagag agtacataag acaaggagat atatatcaag
    14941 ctaatttaac acaaaggttc agtggagaaa cagaacttac aagctttgaa ttgtataggg
    15001 acttaagaag atttagccca gcaccatttg gggcattctt aaactttgaa gatgcacata
    15061 tattatcaaa ttctccagaa agatttataa gatgtgttaa taagagaata gaaactagac
    15121 caataaaagg tactcgtcca agagggaaag ataaagaaga agatttaaga cttcaacaag
    15181 aacttagaaa tagtgaaaaa gatagagctg aactacttat gatagtagat ttagaaagaa
    15241 atgatattgg gagaatatct aaaactggaa gtgttaaagt tccagagcta tttgtaattg
    15301 aaccatatgc aaatgtaaat cacttagttt ccacagttgt aggtgagtta aaagatgata
    15361 aagatgctac tgatgtaata aaagcaactt ttccaggggg gtctataaca ggagcaccta
    15421 agattagagc aatggaaatt atagacgaat tagagccaac gcaaagaaat gtatatactg
    15481 gttcaatagg ttatataggt tttaatggtg atatggattt taatatagca attagaacaa
    15541 taatcaaaaa tgacaaaaaa gtctattttc aagttggagg aggaatgact tgggattctg
    15601 acccagatga agaatatcaa gaaacacttg ataaagctaa atccataatg aaggctttga
    15661 ggggatatta tgaagaataa tgtaggtttt gatagtagtt taagtaaatt tggaattggt
    15721 ttgtttgaaa ctattagagt taaaaatgga atagcagtag atttagatat ccatatagaa
    15781 agaatgctaa gttcaataaa ttgtttggat ttagatataa aatataaaaa agatttcttg
    15841 ataaatgaaa ttgttacata tataaaaaaa gaaaatgtca ttaataaagc acttagaatt
    15901 actgtttttg atgaaggtta taatatatct attagggata ttccatataa tcaagagacg
    15961 tatgagaagg ggtttaaatt agctatatct ccaatagtta gggggaatag tttaattcat
    16021 agacataaga ctactaatta ttttgaaaac atatatacca aaaatatcgc aaataagact
    16081 ggttataatg atggtatatt tttaaattca gaaggtgtaa tattagaatg ttctatgagc
    16141 aatattttct ttgtaaaagg agaaagagtg tacactccta gtgatagatt accgatatta
    16201 aatggaataa taaaaaaaag gattatagag atttgtgacg aattacatat agagttgata
    16261 gaaaatgaaa ttaatatatc agaaattagt agttttgatt ttgtttttgt cacaaatagt
    16321 cttatgggcg ctataaaggt tacagaaatt gacaaaataa aatttaataa agaaaatgtt
    16381 gtttttgata aaatagtgaa gttattagaa taaattaaaa tattaacaat ttgttggaga
    16441 caaaagatga taggttgtat ttatgaagta tacaatgaag agataactaa aataatagaa
    16501 aaccaaaaca ctttggctgt atatttagtg ggttctagta aagacgtaga ttttactctt
    16561 tatgatgtag agataaacga tattgatgtg ttcgtttttg taaaagaagg ggaaaaacaa
    16621 gagagaatac tgtttaaaaa aaagggaata gaatttgatg taaattactt ttcaaaagat
    16681 ggtgtaaaaa aattattatc aaatagggaa tatttctttg tgaaagaaat gaaagatgct
    16741 aaagtactgt ttgataggga gaatatatca catattataa aagatataag tagaaatatt
    16801 tacttagaag gacccagtaa aatgtcatta gaggaaaagt cttttataaa gcaagatatt
    16861 ggagcaaaaa tatcgaattt aaaaaataaa gaaaaatttg atgtttttga atatcatttt
    16921 ttgacgaatt tatatttaaa agatataata attgggtact ttaatataaa tgataaatgg
    16981 gtaccaaaag acaaaaaatt gctaaaagtt ttaaaaaaag aaaataatga gctttttgaa
    17041 ctagtgtcaa aagtaagtga aaattatgac tatcaaagac ttttagatgt ttataattat
    17101 atttttaaag aaattgagac aaaagaggtt ataaaactaa tcttctagtc acaaatcaat
    17161 ggggggagtt taaatgaata aagttgataa agaaaagata caacatgcag taagagaaat
    17221 actagaggca attggggaag acccagatag agaaggtcta atcgaaactc caaatagagt
    17281 tgctaggatg tatgaagaga tattctctgg tcttagtgaa gaaccaagag accatttaaa
    17341 agttcttttt gcagatgaaa aacatgaaga gttagtattg gtaaaagata ttccatttta
    17401 ttcatgttgt gagcatcatc tagttccttt ctttggaaaa gctcatattg cttatttacc
    17461 aaaaggagga agacttacag ggctttctaa acttgctaga gtaatagata cattggcaaa
    17521 aagaccacaa ttacaagaaa gaatcactaa aaatgctgca gatataataa tggaagaatt
    17581 acaaccttat ggtgtcttag tagtagtaga agctgaacat atgtgcatga caatgagagg
    17641 cgtaaaaaaa ccaggttcaa aaactgttac atcagcagtg agaggaatat ttgaaaagga
    17701 cattgcgtct agagccgagg ctatgagttt gattactatg aagtagcaat ttattataga
    17761 tataatcatt tattgagata aaatttaatt taagggagtg tcattaattg tgtttgatta
    17821 tggtaaaaga acttatataa tgggaatatt aaatgtaact ccagatagtt tttctgatgg
    17881 aggagatttt aataacttgg acatagcaat acaacatgca aaagatatgg ttgaccaagg
    17941 tgcagatata atagacctag gaggagagtc tacacgccca ggtcatagct atgtagactc
    18001 agacgaagag ctaagaagag taatccctgt aataaaaaaa cttaaacagg aattggatat
    18061 accaatatca atagacactt ataaggcaga tgttgcagaa gaagcattaa aattaggtgt
    18121 cactatggta aatgatgttt ggggtcttag aaaagataaa aatatggctt ctgtaatagg
    18181 aaaatatgat gcagaagtgt gtataatgca taatcaagat gggacaaact atgacaaaga
    18241 tataatggag tcaataaaag acttttttaa ggtaagtata gaaatggcaa tgagttgtgg
    18301 tgtaaaaaaa gaaaaaatag tacttgaccc aggtgtagga tttggcaagg attttgaaca
    18361 aaatattgaa gtattgagaa gattaaatga attaaaagat ttagggtatc ctattttgct
    18421 tgggacatcc agaaaatcag taataggaaa agtacttcca gttgagccta aaaaaagatt
    18481 agaaggtaca atagctacta ctgttcttgg tataagagat ggcgtagata tagttagagt
    18541 acatgatgtt tatgaaaatt taatggcagc tagaatgact gatgcaatat atagaaagta
    18601 ggtataatat aatggataaa atattactta gtaatctagg attctatgga tatcatggtg
    18661 ttcttaaaga agaaaatttt ctaggtcaga aattctttgt cgacatggaa ctttatattg
    18721 attcaagaga agcaggtctt agtgacgata taaataaatc tgtaagctat gccgaggttt
    18781 acaatgttgt taaagatatt actgaaaata aacaatttaa tcttttggag gctttagcgg
    18841 aaaatattgc tgaagaagtc ttgaataaat ttatacttat aaatggagtt atggtcagag
    18901 ttagaaagcc tgaggctcct gtaaatggta tttatgacta ttttggtgtt gagataagga
    18961 gaacaagaga tgaataaggc ttatttggga ataggtacta acatgggtga tagatttgat
    19021 aatttatcaa gagcatgtga acttttgaaa aatagtgatt ctatatataa agtaaaagaa
    19081 tcaagcttat acgaaacaaa accatgggga tatacagaac aagcagattt tctaaatatg
    19141 tgtgtagaaa tagaaacaga gtttgaacct tatgagttgt tagaatattg tcaagaaata
    19201 gagagagaac tacacagaga gagaatagtt cattggggac caagaactat agatgtagac
    19261 gtactatttt ttaatgatgt agtttccact gatgaaagac taacaatacc tcatccaagg
    19321 attcaagata gagctttcgt tttgatacca cttatggatt taaatgaaga acttataata
    19381 aatgaaaaaa caataaaaga acatttaaac ctcctatctg ctgaagaaag agaagaagta
    19441 aaggagcttg taggttatga aagaaaacct atttaataat aattcaaaga tagagataaa
    19501 tacaaataac aaaaagataa ctgtaaaaaa agataaactt ataatagctg gaccatgcgc
    19561 aatagagagt tatgagcaac ttttggagac tgctaaattt gtaaaaagtc aaggagcaaa
    19621 cattttaaga ggtggagcat acaagccaag aacatcgcca aattcatttc aaggattaaa
    19681 aaaagaaggt cttgaaatat taaaagcagt taaagacgaa gttggtatgg ctgttattac
    19741 agaacttatg gatgtaagag atatggacga actgtatagt ataagtgata taattcaaat
    19801 tgggtcaaga aatatgcaaa actttactct tttaagtgaa gtgggaaaac aaaataaacc
    19861 tgttatgtta aaaagaggaa tagcatccac aattactgaa tggataggtg cttctgaata
    19921 tatagcaata gaaggaaata gtaatattat aatgtgtgag agaggtataa gaacgtataa
    19981 tgattatact agaaatacat tagatttagc tgcggtaccc attattcaaa aggaaacagg
    20041 tcttcctgtt gtggtagacc caagccatgc cactggagtt agatatttgg taaaacctat
    20101 gtcactggca tcttttgcat gtggagcaga tggaataatg gtagaagtac atcctgaccc
    20161 agaaaatgct ttgtcagatg gtgctcaatc tctttgcttt aatgaatttg aagatttaat
    20221 gaaatcaatt aataattatt aaaagaggca agagcctctt ttttattcgt aatcgttgct
    20281 aaggtaaatt tataagttat aatttgatag ttagattatt taatataaat aaagatttaa
    20341 tattgataga attaatataa aaaataaaaa ttttttgatt aataagaagg atatcaataa
    20401 aaaaagtcga attgtgtata ttgagtgatt gacaaattta gaatatagtg tagaatagaa
    20461 atgttaacac aaaaaattac accagaaggt gataatatga atgatataaa agacattatt
    20521 gaagaaataa aagctagatg cgatatagca agtataatat ctgaatatat gaatataaaa
    20581 caatctggag ctaattacaa aggattatgt ccatttcatg gcgaaaagac accttctttt
    20641 tatataaata catcaaaaca gatttataag tgttttggtt gtggtgaagg tggagacgta
    20701 ataaattttg tcatgaaaat ggaaaatttg gattttatgg atgctgtaaa aattttagca
    20761 aataaatgcg gtatagaaat taacactaac atgaatgaag aaactaggat taaaatagaa
    20821 aaatctaaga aatttcaaga tattcatacg gaagcagcta gattttatct ttcaaactta
    20881 ttgggaagca aaaatcttgg gtatgaatat ttaagaataa gaggactaga tgataaaatt
    20941 attaagaaat ttggtttagg attttctttg gattcatgga attcacttat gaatactcta
    21001 atcagtaaag gatataaaaa acaagactta ttagagtgcg gtctaattgc aaaaaataga
    21061 gatggaacta attgctatga taaatttaga aatagagtta tgtttcctat attcgattat
    21121 agaggaaata taataggatt tgggggaaga gtattagacg attctttgcc aaaatactta
    21181 aattctccag acacattaat ttttaataaa aaacagaact tatacggatt aaattttgct
    21241 agaaaaaatt tagaaagtaa aactatagtc ttagttgaag gatatatgga tttaatttca
    21301 ctttatcagt atggtataaa aaatgtagta gcaacccttg gaacagcctt gactgaacaa
    21361 caaggcattt taataaagag atatgcggac actgctatta tatcttatga ttctgatgaa
    21421 gcaggaataa aagcgacttt aagagctata gatatactta ctaaattagg tatcaatgta
    21481 aaagtgttag acttaaaaga tgctaaagac ccagatgaat ttgtaagaaa atacggactt
    21541 agtgattata aaaaagctat ggatgtttct actcactaca taaaatataa aatagatcat
    21601 cttaaaaaag aatttaacat tcaaaaagat gaagaacgag tgaagttcgc aaaagaagct
    21661 tctaaaatta taaaacaatt gacaagtcct gttgaaattg atttttatac aaagtattta
    21721 agcaatcaaa tagatattaa tgtcgagtct ataaagagag aagtttatgg taaaaattac
    21781 aataagccat ataataacaa aaatcaaaag aaaatagaag aaaaagtaat tgaaaaagtc
    21841 gaagtcagac aagacggaaa acaattagtt gaagaaactc taataaaaat aatgttagaa
    21901 gacaaaaaaa ttagagaaat agcgttactt aaagtagaag aaagtgattt tttattgaaa
    21961 gaaagtaaag aaattttaaa ttacatgatt aaaaatcaag aattggacaa aataactatt
    22021 gacaaattga aaagtttaaa catatccgaa gaatacttaa aagaattaaa ttcaatttct
    22081 ttgaatagta taaacttaga gaacacaaaa gaaatagaag gaataataac aaatatcaga
    22141 aaaaactcat tagaagagca aattaatagc ttgttgagag aacaacaaga attagaaaat
    22201 aataatgata tgaaagaggt agatggtaga gttatggaaa ttgctttaaa aatagttgag
    22261 ataaataaaa ttttaaagag cttgtagaga aaggaggttt gagtgtggaa aataaatcga
    22321 ataaaaaaga gttaaaaaag gttactgcta agacattaat agaaaaagga aaaaagcaag
    22381 gttctctgac acttgcagag ataatggaag ctttttcgga gactgaactt gataaggatc
    22441 aagtagaaaa tctttatgag actttaggaa atttgggaat agaaataaca gaaacaaaaa
    22501 actataaagc tgatatagat ttttcggttg ctgatgacga tttaagtata ggccacttag
    22561 atgaagatgc agaggcaatt tcacatgatg actcttctgc aatagaaata gaaactgtgg
    22621 atttatcttt accaaaaggg ataagtatag atgaccctgt aagaatgtac ttaaaagaaa
    22681 taggaaaaat tcctctactt aaaccacatg aagaagtaga atttgctaga aggatgcacg
    22741 aaggtgatga gatagcaaaa caaaggttag ttgaagcaaa cttaagacta gttgtaagta
    22801 tagcaaaaag atatgtagga agaggtatgc ttttcttgga tttaatacaa gagggaaatt
    22861 taggtcttat aaaagcagtc gaaaaatttg actatacaaa aggatataag tttagtactt
    22921 atgcaacatg gtggataaga caagctataa cacgtgctat tgcagaccaa gctagaacta
    22981 taagaatacc agttcatatg gtagagacta taaataagct aataagagta tcaagacaac
    23041 tacttcaaga acttggaaga gatccaaaac cagaagaaat tgcaaaagaa atggaaatga
    23101 cagaagataa agtaagagaa ataatgaaaa tagctcaaga ccctgtgtct ttagaaacac
    23161 caataggaga agaagaagat agccatttag gtgactttat tccagatgat gatgctccag
    23221 ccccagcaga ggcagcagca tattctttat taaaagaaca aatagaagat gtacttggtt
    23281 cattaaatga tagagaacaa aaagtattaa agcttagatt tggtcttgag gatggtagag
    23341 ccagaactct tgaggaagtt ggaaaagagt ttgatgtaac tagagagaga ataagacaga
    23401 tagaagcaaa agcactaaga aaactaagac atccaagtag atcaaaaaaa cttagagatt
    23461 atttagacta aaaagataca tcattgatgt ttttgtgata aatgccatga gataagtgat
    23521 atctcatggc tattttgtag taaagattta attttatttt tgtagttatt tcagtctaat
    23581 atctataact tggattaaat cctataaata aagggaattg aaaggagaat tatatttgaa
    23641 attaacagat agattattga aaatagcatc attagtgtca gatggtaaaa aaatagctga
    23701 tataggaacg gaccacggat acataccagt atatttgtta aaagagggaa aagtaccatt
    23761 tgcagtgcta gcggatgtta ataaaggacc tcttgacaat gcacataaag aagtcataca
    23821 gaacaatctt ttagataaag ttgatttacg attgggttct ggtatagaga tacttgaaat
    23881 aggggaagta gaagaagtga taattgctgg tatgggtgga atcttgataa gtgagcttct
    23941 cgaagctaaa aaagaagtag cacataatgt agaaaaatta atattacagc caatgcaagc
    24001 acaagaagaa cttagatatt accttttaaa caacggatat gaaattttag aagaagtttt
    24061 agtgagagaa gattttagaa tatatgagat aattgttgcc aaatatacag gtaaaaatac
    24121 tataatagaa gatgagatat attatgaggt tggaataaag cttttagaaa acaaagattc
    24181 tctttttaat gattttatag aaaaaaagat aaagacatat agttctatag ttaataaatt
    24241 agaagggaaa aatggggaag ctatagataa gaagagaaag gaaagcgaag ttgcaataaa
    24301 aaaacttgaa aatttaataa aataaatagg aggtatatat gctattaaag agtctaacta
    24361 gaaaaattga aaaaaagtat cctctaaact tagcagagga ttgggataat gtaggtctta
    24421 tagttggaga ttttgatatg gatgtaaaaa aggtattggt atctttagaa gctaatgaag
    24481 atgttattga tgaagctata tccaaaaata tagatttaat tgtgacacat catcctttta
    24541 tatttgggaa aattaataaa attaatagtg gagatttaaa aggcagactt atccaaaaac
    24601 ttattaaaaa tgatatatca ctttattcta tgcatacgaa ttttgatata gcatttgatg
    24661 gtctaaatga ctattttatg gaaattatgg aatttggtaa ttcaaaggtt ttagatataa
    24721 ctaaaagcga aaatttgtat aaattagcag tatacgttcc acataactat tcagatgaac
    24781 ttagaaaggt tttaagtaat tcaggagcag gtcatatagg gaattatagt gattgtacgt
    24841 tttcaacaga aggtgaagga caatttaagc cattagaagg ctctaatccg tttcttggaa
    24901 gtgtaaatga tatagaaact gtaaatgaag ttaaaattga aactgtagta ccacagaagc
    24961 ttttaggtgg ggtaataagt tctatgttag atgcacatcc ttatgaagaa gttgcatatg
    25021 atttgtacaa gttagaaaat aaaggtgaga tatttggact aggtagaata agtaaacttg
    25081 ataaaagtat gacattagaa agtctttcaa ggaaaataaa agagaaatta aatatgaaac
    25141 acataagagt agttggaaac cttagtacag acataacgaa agtagccgtt gtaacaggag
    25201 ctggttcaga atttgtaaaa aaagctaaga gacaaggtgc agaagtacta attacgggtg
    25261 atgtaaagta tcatgaggca caagatgcac ttgatattgg aatgtgtata gtggattgtg
    25321 gtcattttga tacagaagat atattcaaaa atgttatgaa gagattttta gatgaattta
    25381 gtgaaattga agtaataaag agtaatgtat atttgaatcc atttagtact atttagcagt
    25441 gtttgataat aaaaatacta tataaaaaat aaatttgagt ttaattaggg ggataatgat
    25501 gaaagtagca gtaatttttc atagtgtttg tgggagtaca tatttattag ctagagaata
    25561 taaagaagcg cttgaagaaa tgaatatcga ggtagatata tttagagtaa gtgacgaagt
    25621 tgcaaaaaca cttcctcaat attatttgat aaattccaaa gaatacaaag acgaatttga
    25681 aagtataaat gtaattaaat ctggaaaaga aatattagat tatgatgcta tatttatggg
    25741 ttcaccaaca tattatggaa atgtatctgg accaatgaag atgtttatgg atagtttttc
    25801 tgatatttgg gtaggtgctc ctttgagtgg taaaatattt ggttgttttg ctacagcagg
    25861 ttcacaacat ggaggaggag aattagcttt gcaagctatg aatatatttg cccaacatat
    25921 gggaatgact cttttgtcag ttccatgttc agtaagagga ggttatccag cgtatggaat
    25981 acttcatata gcaggagata attctgatat aaggccaaat gatgatgcca agataggtat
    26041 aagagattac ctaaaaagac ttaacatata agaatatcat ataaaaatac ctaaaagcga
    26101 ctgtatatac ttttaggtat ttttacttaa ttataaacgt gaagctttac cagaaatatg
    26161 gtcaatttct attttaacaa cagttgttaa gttcatatcc ttctctatgt acttcatgcc
    26221 ttcttcaaag aatcctttag aatatttttt tattatttct acaagagcat ttttcttttc
    26281 ttcattttct acagtgcaag cttttccaaa aactatagca ctagaataag tagtactaaa
    26341 tttatctgga ataacacttt cattagcaac tactaaaaaa gaaactttgt tgtttttcga
    26401 tatattatct aacttatgcc catttcttgc acaatgaaag taaatagaat cattaaagta
    26461 tacataattt acagcaactc cataaggata accattttca gatatagtgg aaaaagtacc
    26521 aaattcacca ttttttaata cttcaacagt atcttctttt gtcatttctc tttttttcaa
    26581 tctcatttct ttaaacataa tatcatcctt tctaattttg atgcttaaat taaattttag
    26641 aacatttata aagattatgc tatgtttttt acaaatttta agaaaaaaat atagtttaag
    26701 ataaaaggta gtgaaagaga agctatttgg aggtttattt atgatattat tgttagagta
    26761 gtacaaataa tactaataaa tttaatagct ggtttcttgt tatttaaggt agtaagcatg
    26821 tctataagaa ggggaagtta tcgagggtag aagttgttgt ttataacgat attataaaga
    26881 aaatattatt atataaaata ataaatagta aagaggtatc aagttgaata taatacttaa
    26941 tacctcttca aaattaagtt ataaaagtgt tgtgggaatt gtcctataca tggtaaggag
    27001 ataataaaga atgtatcttt aaagtctcaa ctaaacagca aaatttatcc aacaaacata
    27061 caataaatgc ttctttgtat ctaggtggct taacagtaag tggccacatg tgttttaaaa
    27121 ttatgtccgt ttctttttca tttatttgaa aaaataaagt tgcatttttt aatgcttctc
    27181 tagggtgagt aaatccatgt aatccttttc tatcaccttt gtaatgccaa tcataaagaa
    27241 aaaagtcgtg tagtaaagct cctctaataa cactttgaat atcaacactt aagtgcaatt
    27301 ttttacataa tagataacta taatatgcta ctgacaaact atgttcaaga catgaaacat
    27361 tgccatgatg aggaaatgtt ctcataagtt gaactttttc agaagaaagt atctcagaag
    27421 cacattcatt aaaatatttg atttcagctt ttgacaacat tatgaaaatc tccattctta
    27481 gtaatatata aatactatac ttcaacgata atatgaaata tttcttatcc tacaaaatat
    27541 aatacccaat aatacaagta agataccttt tatggtattt taaagtaaga gagattttat
    27601 taggctaaac aatgaaaaaa ttaaaagtaa agctatagca atatatagta tttttccaag
    27661 aggattgcca aaatttatag tccatccaat accaaacctt ttattaacca ttaatgatgg
    27721 gtcatttgga ttattataca tgatgcctgc tatccaatac ttctcatctt cttctggaga
    27781 ataagatgag ttgaatttta aatttgggga attaatatat gttgctatta aataaattat
    27841 aactaaaagc gttgtaatat tcatagcagt taatagtttt gtttttattg ataaaagagt
    27901 agtaaaaaac tgtgttgtca ttataatcat caatattaaa aacaaataac caattttatt
    27961 taaatattta atgttttcaa ctctactttc ttcaattcta tttgtatcta tcttaatcct
    28021 tgactttatg gatccaatgg aagttatata taataaaacc attaaacaaa agtcagtacc
    28081 gattaattta aatacattaa gaaatgattt atctataaaa acatcaggag acccatttat
    28141 gttccaatga gtggcaattg agtctggcaa ttgattataa ttaaatgcta taaaaatact
    28201 cattcccaaa gtaaatagta tcggtattaa atagattatt ttaaattttt ttattatttt
    28261 atttttctta tttataaaat ccatatctac tattaatttt gaatctatat ctatatgacc
    28321 taatttagca taaatttctg atttaagctt cttagctttt ttatgtgtat gaatatatag
    28381 tatactttca tataataaaa aacctataat accaataaaa taggaaagtt ccaatttatt
    28441 aaatgtaaag accagaataa aaactaatat aaagtctata ataaaaccta aagataatag
    28501 ttttttaaat tctttatcta actttataaa gtaattataa tttttatgct cttggtctat
    28561 agaaactcca tagatgtgtt ttttacctac aaaatagggt ataaaataaa atgtagcata
    28621 catcaaaatt agaactggaa atattgtaaa tagacattca atcttactca taatatttca
    28681 cctcatactt gtcaaataaa cttgaactta gtgacaaaaa ttcttcttta ctaatacctg
    28741 ataaataaga ttttgccacc aataattcca agtcaagttt atttttttga cgagttgtat
    28801 tatctatttt cattggtagt tgagctacaa tagcaccttt tcttctatca atatttaaga
    28861 atccctcttc ttttaattca ttgtaggatt tgttcactgt atgtaaattt acaccaatat
    28921 tttcagccat acttcttaca gatggaagtg attcatttat tttcaattct cctgaagcta
    28981 tagccttagt tatttcttct ttaatttgta cataaataga tttatcacta ttaaaatcta
    29041 aatttaaaac cataaaaaac ctccattatg ctttataaat gaaaacgatt gttatataca
    29101 tagtataaca taaaaaataa taacaaacac aaaatataag tttatatttt gtaaaaattg
    29161 taatttaaaa aataacataa tttttattaa aataatacag atactattta tagatagaaa
    29221 taaaaattag aaaggagaaa aaatatgtta acatatttaa tgaaaagaca aatgcataaa
    29281 aaagatgatg gaatgagtaa accaatgatg attacaggag ctgtaattac aggtgtagga
    29341 gcatatgctg tatataaaat ggtaaaaagc cataaatcta attctcaaat ggtcaataca
    29401 aattattatg gaaatagcga ttatgatgat tataaatatg acgaatttga ttatgactgt
    29461 gattgtagag acacaaaaca agattatgaa tatgaagaat taaaaaaaaa agtaaaagaa
    29521 tttaattcaa gaagaataaa ttgtgaaaat tgtccacatg tttctcaaga agaaatgatg
    29581 caatatgtaa atagtgttaa agatgtaaag gaagagcttg atttacatga agatgctaaa
    29641 aaagataata tttacaaaga agatgaatac aaaaaatatg aagaagaata agtaatattt
    29701 tgtataactg aacaaatagt aatgtcaaat tatttaatat ctagttttaa tatgtaagtt
    29761 gtaacttaac ttgaaagaat cagtatcatt ctttagatag ttaagtaaca acttacattt
    29821 tatttttata aattttaata gttctttgtg actattttgt aattttagaa atattttagt
    29881 atccagggta attgtgtata ataaaacttg tatagataga tagtatgaaa ttaagcagat
    29941 agtttaaaat taaaatgaaa agaaaggaaa tagttatgtt aaagttgttg atagttgaag
    30001 atgatagtac tatatctttt ggaataaagt atgctttaga acaagaagga tttagtatag
    30061 atatatcaaa ggatttatct agtggaaaag aaatgatttc ttctaatgaa tatagcatga
    30121 tactgttgga tgttacattg ccagatggaa ctggttatga gctttgtcaa tatataagag
    30181 gattctctca ggttcctata atattcttga ctgcttgtga tgaagaagta aatattgtaa
    30241 tgggtctcga tataggtgga gatgattata taacaaaacc ttttagaatt agagaattaa
    30301 tatccagaat aaaagctgtt ttaagaagaa aaggtaatac ttcagaagaa aataagaaaa
    30361 tacttaaatt tggagattta agtatatata cattagaagc tagagtatat aaaaatgata
    30421 aagaaatatt tctgacatca gttgaatata aacttctttt aattcttata cagaataaaa
    30481 atactgtttt aagtagaact caaatattgg aaaaactttg ggatgtaact tatgattttg
    30541 taaacgacaa tactctaact gtttatataa aaaggcttag agaaaaaata ggtgatgatt
    30601 tatgtgaacc aatctacata gaaactgtaa gggggattgg ttataaatgg ataggaagtg
    30661 aaaatagtgt ttctatataa tccagaagtg aagaagtttt taagtaagta tgttactttg
    30721 atgtttattg ttatcataat atctattgga tttagcataa tcaacgtgtc cttaactaaa
    30781 gagatggttg taagaaataa tcaggctata ataggaactt tatcaagcaa gtatccaaac
    30841 ttagaaagtc agatagtaga cataataact caagggaagt ctatggagaa catagattat
    30901 ggtcagaaaa tattaattaa gtataattat gataaatcta ttaggataaa ctctgagcct
    30961 attatttcta aattagtttt agagactatc aaaataaata taatattagt atgtataata
    31021 tttattttga tatttgtgtt gattgttaga tattttacgt caatttacaa tgatttatca
    31081 gatatgacta agtatgttta ctatagttca gagggtaagc catttgatat gaaaaacaag
    31141 aatcaagaag ggcagatagg tcttctaaaa actgaactct taaaaatgac gacaattctt
    31201 aatgaaaagg ttgaactttt aaaaagagaa aagatatttt taaacaatac aatttccgat
    31261 atatcacatc aattaaaaac tcctatgact tctttaataa tgttgaatga tttattatat
    31321 aatgatgtac catacgaagt aaaaatcgat tttttaaata agataaaaaa tcaattaaat
    31381 agaatggatt ggttgattaa aagcatgctt aaattatcaa aggtagaagc aaaagtgata
    31441 aattttaaaa aagataaggt aaaatttagt gaactcatac atagagcaat gctatctatg
    31501 aaaataccta tggagattaa aaatcaaaaa ctgactatag agggaaatga taatgtatct
    31561 tatattggtg atattgattg gtccgtggaa gcattagtga atataatcaa aaattgtgta
    31621 gaacatactc ctgaatttgg aaatataatt ataacctata aagaaaatcc attattttct
    31681 gaactaataa taaaagatga gggtgaaggt atagataaaa aagatatacc acatgtattt
    31741 aaacgattct atagaggaag aagtagttca aaagaagata gtgtaggaat aggtcttgca
    31801 atgtcaaaat ctataataga aagccaaaat ggcgatatat atgtaaacag cgaaaaaggt
    31861 aagggtacag aatttcatat aatatttcat aaaatgtatg atagtgacta atctgtaata
    31921 tttaattcac tttatagtaa tagccaaaac ctaatatatg aagtatagaa tataaatatt
    31981 ggaggtagtt atggaaattt taagagtaga aaatctaact aaaagttatg gtaaaaatga
    32041 aacaaaagtt gatgctataa aaaatgtaag tttatcagtt gaaaaaggaa cgtttatagc
    32101 aataacaggt ccaagtggaa gtggtaaaag taccttatta catttattag gaggagttga
    32161 cagaccaact agtggtaagg tttatataaa tgatgttgat atatataatt taaaaactaa
    32221 ggatttggcg atatttagaa gaagaaatat aggtcttata tatcaatttt acaacttaat
    32281 tccagtctta aatgtgaagg aaaacatatt gcttccagca gagcttgaca atagagatat
    32341 tgatagagaa tattttgatg atttaatgaa aacattaggt ctaattgata gagaaaagca
    32401 ccttccaaac caagtatcag gaggtcaaca acagagaacc tctataggac gtgcattaat
    32461 taatagacct tctataatat tagcagatga gccaacaggt aatttagata gtaaaaattc
    32521 aaaggaaata ttagaattgt taaaattgtc agtaaaaaaa tacaaccaga cactaataat
    32581 gataactcat gacaaaaata tggcacttca agctgataga attataagca tagaagatgg
    32641 aatcataaaa agtgatgagg tgatgtagat gaatttatat acatcactaa ctattagata
    32701 cttaaaacaa aataaaagaa gaaccattgt aaccataatc ggtataatac tttcaacagc
    32761 tttaatttgt ggtataggaa atatatttga aagttttatg gattatcaaa tgagagaaac
    32821 tataaagaat gatggaagtt tccatgttac attttatgat gtaaatagaa aaaatgtgga
    32881 atatgtaacg aaatcagctg aaatagaaaa acatgcattt accaaacaac ttggatattc
    32941 taaattagaa aatagtgaaa atgctatatt aagtataaag caatatgata aaaatgcaat
    33001 aaatggatat aaagtttcta taaaagaagg tagatttcca gctaaggaag gagaaattgt
    33061 tttaagtgaa agtattatta atcttataga tgataagtta aaaataggag ataagataac
    33121 tttaaaagta ggggatatgt ttgactcaag caagaaaaag attgatagta tgacatttca
    33181 tgaaggtgat tatatagcaa atgaaaaaga tagaactttt aaagttgttg gtataattga
    33241 aaaacctggg tttgaacgtt ataatggaat tgcaacagct atagcctact ttaatcctat
    33301 gaataattac aatgatacaa ttaatgtatc agtagcagta aaaaatccta aagatgtgta
    33361 taagataagt aataaaataa gtaaaaatat atattcaaat aaagataaag aagcagtttc
    33421 tgatatgata aaatacaatg aacatttatt gaggttacaa ggtgctagta agtattcaaa
    33481 tataaatagc tctataaagt ctataattat agcagtaact attttagtaa tcgtatgtac
    33541 aattgctaca gtgtacaatt cctttagtat ttctataaat gaacgaaaaa aacagtttgg
    33601 gatattaaat tctataggtg ctacatctag tcaaattaaa agattagttt ttatagaagg
    33661 tattataatt agtttaattg gtataccaat aggactaata tctggaacag tagctataga
    33721 cttattgttt aaaattataa ataagtattt tactgaatcg gttgttacta agatgagttt
    33781 gcagatagtg tataatccaa taataattat tgttagtata ataatagttt tgcttacaat
    33841 ctttatatca attctactac ctgcaatatc ggcttctaat atttctcccc ttaatgtgat
    33901 taaaaatact ggagagtata aagtgggcaa aatcaaaagc tcaagactta ttaaaatgat
    33961 ttttaaaact gaaggagtgc ttgcatataa aaatattagg agaaatagaa aaaaatttat
    34021 aataactctt ttttctttaa tggtaagtat aataatattt atatctttta gtggctttac
    34081 attactttta ctaagaggtg aagaaattag aaattctcaa agaaaatatg atttgtattt
    34141 aactgcaaag aacacagcta ggtctgtaga ggatactata agtgaactag aagatattga
    34201 tgggataaaa aactttgaat tggcaacagg acaatatgtg tctataagag taagtgaaaa
    34261 taaaataaat aaatccaagg aagatttaat caggaaatat tatcaaaaat ataagattgg
    34321 agatagttat gaatatgatt tcagtaataa tgaattaatt tttccaggag attttgcagt
    34381 aaaaaacaaa ataaataact tagtacaggg ctcttttaat aaagagagag caattgaaga
    34441 aaatggtgtt atattagtta gaaaaagtgc aattgaagaa aatgggaaga aaggtgtagt
    34501 agaacttacc aactataaag taggagatac agtaaattgt gaatatcttg atgaaaatgc
    34561 cttaagtaaa aaagtaaagg tcaagattct tgctataact gacgaagaac gtctaggtct
    34621 tggttatcaa aatatggggt tacaatttat aacttatgat gaagttgcta aaaatttaaa
    34681 tttaaaatta agtaaaagcc ttatatttat tgattcagga ggaagcatta agactaagaa
    34741 ggaaatagaa gctttggcaa ataaaaataa ttttaacttt tatgatgaaa gtgcaagcaa
    34801 tgaaagtgaa aaacaagatt taaaggttat taaaatattt gtatatggtt ttatagtagt
    34861 gatatcttta gtgagtgtta caaatattct aaatacagta agtacaagta taaatctaag
    34921 aaaaagagaa cttgcagtaa tacaatctat aggggttact cctaaaggat ttaggaaaat
    34981 gatatattta gaaagtttta tatatggtat actgtcattg ttgtttggaa taccaattag
    35041 tattggtata attctaatta tgaataaact tatttcaggt gtaattgaat tttctccgat
    35101 tataccgtgg acagccatag ttatctgtat agtaagtgtt tttataataa cttttatagc
    35161 agggtatata ccaatgagta aattaaataa agaaaatata atagataata taagaagaga
    35221 aagtatatag atataaaaat aattcttaac tatataaaat attattaaaa tcatttaagt
    35281 ggtataaaat attattatat cacttaaata attttttatt tatataagaa atctaataat
    35341 aaattactag aggtatttaa ctatttagaa agaattggta ttatataaag taagagaata
    35401 tataataaga aagaatattt gattttattg gaaaacaaaa gaaaaagaag gtgattacta
    35461 tttctcatat aactgcgaag aatatgtata aaagtttaga ggaaagaata aacaagtttc
    35521 cacaaggtgc tccaccatct gatacattat acaaaatact aaatgtacta tatacagaac
    35581 aagaggcgaa gttagttgct cagcttccaa taaagccttt cagagtaaaa acagcagcta
    35641 aaatttggag tgtaagtgaa agtgaagcat atagagtatt ggataaactt gctagtaagg
    35701 ctctgatact agatatagaa gataataaag ggaagaaata tataatgcct cctccaatgg
    35761 ctggtttttt tgagtttgcc atgatgagaa cgagacacga tatagaccaa aaacttttgg
    35821 cagagcttta ctatcaatat atgaatgtag aagaagattt tatcaaagat ttattttatt
    35881 caacagaaac gaagcttgga agagtatacg ttcaagagga agttttaact aatgataatg
    35941 aggttagtat tttagattat gaaagggcta ctcatataat tgatgaatca actcatatag
    36001 gtataagtat gtgttactgt agacatagaa tgcaacatgt tggaaaagct tgtgatgctc
    36061 ctatggatat atgtatgacg tttgacaatg tagcaaattc tttaataaat aataaatttg
    36121 caagacgtgt ggataaaata gaatgtaaag agctacttca tcaagcatat gaacataact
    36181 tagttcaatg tggagaaaat gttaggaaag gggttacgtt tatatgtaat tgctgtggat
    36241 gttgctgtga agcaatggta gcagctaaaa gatttggaaa tcttcatcca gtacagacaa
    36301 caagttttat accaaatata aaccatgaaa attgtgtaaa gtgtggtaaa tgtataactg
    36361 catgtccaat agatgcgata agcaaggtta aggaagatgg taaagaatat ataaaaattg
    36421 atgaggatag atgtttaggt tgtggagttt gtgttagaaa ttgccataaa aatagcatta
    36481 tgttattaaa gagagatgaa aaaataataa ctccagcaaa ttcagtacat agagcagttc
    36541 ttatggcaat tgaaaaaggg cagttacaaa atttaatttt tgataataat gctctggcta
    36601 gtcatagagc tatgggagct atactgtcag ctatattaaa gcttgaacca gctaaaaaga
    36661 ttttagccag taaacaatta aagtcagtat atttagataa attgttaagt atgaatgata
    36721 aatagaaact aacttaaaaa aggcaaagtt ttagacttat ctatatctta ataaacttat
    36781 catttaatat ttaaatttaa tttttacaca tataaaactt ataacttaaa taaaaaaagg
    36841 ctatattcaa ttaatattac ttgaatacag ccttttttag ttatgttaag ctatttcttt
    36901 tatttgctta aatgagtttt catttccatt tccaccaatt tgtgttacag atttaaatct
    36961 catagtttta aatagttctt tttgattata atctagtgag tttcctacta gcattacagg
    37021 agatttagtt tttccagcca aaactccaac agataatccg tctattaaat catcttgttt
    37081 attcattcca tttttagcaa catataaatt gtctatctta gaatccttgt aaaattcttt
    37141 tattactttt gcattagttt catttctagt gctacctgat attctctgtg gattttgaag
    37201 tttactttct atatttttag aaactgtata ttctccaccg attacataag atttatcaat
    37261 ctttttgttt ttaaacaagt tatttatttc agttatatta ctattttcat tagtaagtat
    37321 tataggcata tcattttgag ctgatacagc tccaatgctt actgcatcgg ctaatccttt
    37381 ttcgccatta actactgaaa tctttgatat attgctaata ttgttcaatt ctttagcaat
    37441 gttcatagaa gtttgatatc tatcacttcc tgaaattcta gatacagaga tattgtttga
    37501 ttttatagta tctaaagatt tttcatttat agaagcttct ccaccaacta catatacatt
    37561 tttaactttt aatctcttta attcagccaa agttttttca tttatttgag agcttccagt
    37621 caataatata ggagcgtttt tcttgtatgc aaatggtgtt gcagccaaag catctgcaat
    37681 tgctgaatcg tttattaaaa ctgcattttc agcattttcc caacctgctt gacttatctt
    37741 aatagaagtt tcatatctat cagttcctgt aagtgcactt gcatttactt catcttgata
    37801 tgtaacctca aatctagcat ttggacttcc taagaaaaca attacgcctc cttttggcaa
    37861 tctaactcta ttgttctttg taactaaaga atagtttaca ggaacacctt tatcatcata
    37921 aacagcaaaa gctgaatttt gaggaagatt aacttctatt tttttatttg cgatatcgtc
    37981 accaatctta taccattttg cataaccatt ggattctagt tcacaagtaa aagatttttc
    38041 tgctggcaaa ttagttatag aatcttcact aacatacgtt agtgttccaa aagataaata
    38101 ctctgtacca ttttctttat gaagtttaat gtcggataaa tcacgaccta ttacaccagg
    38161 tatttctata aaagcatttg agttattctc atccattatt ttagtgttaa ctatataacc
    38221 tggagtttca tcagaaagag caagagttgc cttcacagaa ccaaacatat agctttgaga
    38281 agtatatttt tcatctacta gatagtaatc tttaccattt ctttttttcc atgcttcttt
    38341 tacactattt gatatattgt tgtcatctat cttttgagct acatagtata aacttgctgt
    38401 ctgacctaat ccagggacat catcataaga gctcatattt aaatatgtga tattgttttt
    38461 ttcttttaca aattttagac agctgttacc tttttcagat acaaatctat cttgacctat
    38521 gtatacgtat ttatcttcag gtccattttc tatataagga gaagatactg ttaaagtacc
    38581 attatcatta acatctactt ttatcatatt tgaagaagca taaagaccac tattttcttt
    38641 tagagagcta ggcattttaa cttgttgtgg tttactaaat gttttatcag gttttatttc
    38701 tttaatttta cctttttctt ttaatgcaga tagtaagatt tcttgtccaa ttatttcatt
    38761 aagttgacta cttccaccag aagaaagaac tgcaactgcc atattttcat ctggaagaac
    38821 tataagatta ctgtggaata ataaagagtc tccacctttt gtaagagctt ttaaattata
    38881 ctgattaaat ggataagtgt tgacacaatc ccaccctaaa ccatatccaa gtatagagtc
    38941 ttctccttca ggccatagac catttaaata ttctttgttt tccatagctt ttactgaagc
    39001 aggagaaaga ataccatttg aatttttcat aaatgtttgt gcaaatgtac atagattttc
    39061 tgcacttgaa tataaaccac ctgcacctat agcattaagg ttatcttgcg gaactgcatc
    39121 ttcccaataa ggcacgtatg cttttgctag ttttgaacta tcaaaactat tttctgtagt
    39181 ttttgtattt tgtaagttaa gtggattatt tatgtattta tcaagaaaat tagtaaatga
    39241 cataccagat actctttcta ctaaaatttc agctaaagta aatccatcat tacaataaac
    39301 ggaaaaagct cctggtttag cttttaatct ttgtttttgt aattctttta agaaattatc
    39361 atgaccataa gaatcattgt ctgctaataa aatagtgttt ttaaaactac ttcccatcaa
    39421 ccctgatgaa tgatttaata acattcttgg agtgatttcc ttgtatctat catcagccat
    39481 tttaaattca ggtatatact ttacaacagg agtatctaaa tttaattttc catcatcaac
    39541 caacttcata acagctgttg tagtaaacat tttactgata gatgcgatac tatacatatt
    39601 atccttattt aagtttttgt tatcctgttt gctgtaaact ccaccattac cagaaatctc
    39661 aattttgcca ttatcaatca aagcatattg agcacttaca gcaccatact ttgatagcaa
    39721 agtttggatt ttctctttag caagagcttt agtttttgtg taagattccg cgtttttact
    39781 ataatcatgt aattctatat cagaactttg atatttttcg gaattatctt ttgtattatc
    39841 agcaaaagta atatttggag cagcagtaaa taacatactc aatactaatg tactagataa
    39901 taatttagag agcctttgct taaacatatt acattacctc tttccctttt taataattta
    39961 taattagcat aacattctta attattttaa tcaacagatt attatcaata aaaaaagctt
    40021 ttataagtat agctttttat ataacagaga aaaatataaa aataatattt gttgttgtat
    40081 aaagaataaa gggtataggt aatatataaa taaaatggag gaattttaga caatgtataa
    40141 gaatataaat aaaaagcaac ttaaagaaat gatgaaaaat gaaaaggatg ttttattatt
    40201 agatgtaaga actaaagatg aatttaagga atataaaata gaaaatgcta taaatatatc
    40261 tttacaagag ttaatcaata atatagatga aatttacgat tataaagata agaaagtagt
    40321 agtttattgc agaagtggtc atagaagtgt tactgcatgt aatttgttag ctgaagaagg
    40381 ttttgagcat ttatataatt taaattcagg aataattgat tatatgagtt aaataatgta
    40441 agttatacat taaatactat gtatagctct gcttattaag taggaatata tctaaatatt
    40501 tataagataa atttaggaaa gaacgaattt tttcgttttt ttctatgaca gtttttgtag
    40561 taaatattag tttttaagtt ggaaaaaggt agtagctttt tagctactac cttttaatat
    40621 gaacttagaa tattattaaa aaaaatatat ttaaagacag aattaatttt ttaatgacca
    40681 gttaaattac tatcgccgaa tttatcatta aatggtttta taactctatt tataggtcct
    40741 tttagaagta catttaaaca aatagacaga atcataaata gtaatagaac tagtatatct
    40801 ttagttagat ttggagcata tataccacca actgcttccc ttgtagcacc tattgcatag
    40861 gtaaatggca ttatagggtt tattctttgg aagaatgtag gtgtaacctg tatagggaat
    40921 gtaccaccag aagaaccaat ctgaacgact aatagtacta ttgcaattgc ttttcctaca
    40981 tttccaaata cagaaactaa agaatacact ataaagttaa ataccatact agtaaacata
    41041 agcagaacta tcaataaaat tggatgtttt gctgttactc ctaacaataa tatatctcca
    41101 gttcctatta taaaaccttg gacaagagct atacttaaaa aagtaaggcc tcttccaaag
    41161 taaatttcat aaggtttata gtttccatgt acagatgtag acaatagtgc tgaaataagt
    41221 aatataccaa cccatgtaga aagaacacta taaaatggtg tcattgcaga accataattt
    41281 tcaattggat ataatttctc tgttttcacc tcaacaggtt cttttaagaa attagacttt
    41341 gtcaatatat cactttcaag caagctgact aattttttca tatcttcacc attactaatt
    41401 ttgcttaaag tatcaatcaa atcatccaac attccttttg ctaaaggaag tctttctctt
    41461 atcaatgata tactttcctg tgcatttcct gaaagtttta gagaagtagt aagtatttct
    41521 tcaacctttg gaagttttgc ttctgcctta tctaaaacag taattatatc atttgctact
    41581 tttatactgt ttgcaaatat actatttata ggttttgata tttttgagtc aaaattattt
    41641 aaaatattta aattaattct tccaatacca tttgatagac ttaaaacatt attcaaagca
    41701 cttatagatg gttgttgtcc agatataact ttgtttttaa tatcatttaa tgttgatata
    41761 gatgaatcta ttttattgct agagtcttca agattatcta ttacatcatc caatctatta
    41821 tttgatgtaa gttggtttaa tttagttaaa aagtccacca aagtatcgtt taaagattgc
    41881 aaattagaaa gtttttctga taaattatct atcaacttag gtacatcttc actgccacta
    41941 tttactgcat caattaaatt taatgtaaga gaagaagcag aagatgaaag gtcaaccatt
    42001 aaatttaagt cagacttaat aagaggagaa agttcgtcta aattatcatt tgtatcctct
    42061 aagaattttt ttaagtcact tgataatagt tttgtatcat tcagagtctt tttaattgta
    42121 ggtaaatcag atttaatatc tttaactgta tcagataatt ttgaagtagc atcagaagct
    42181 aaatcaacag tcttatttat atggtcaaat ctcttttgaa ggtcaataag ggaattttca
    42241 acttttgtca attttggaag actgtgttca attcctgcac caactccgtt tgatatacca
    42301 agaacgattt cactaactgt ttttactata gtctgattaa cctgtagttg tattgtagaa
    42361 gcacctttat ctgtaatctt tggtgcaact gcatttattt tttcattaac agtatagata
    42421 atttcgcctt ttttaacatc atctgtaatt aaagatgtca aatcttttgt aaaatttttt
    42481 ggaatcttaa gtgatgcata ataagtacct tttttaacac cttcaagagc agttttttca
    42541 tctacaaact gccaacctaa actcttgtta tctttgagtt gttcaattaa ttggtctcct
    42601 acattaatct tttgtcctgc tatttcattt cctttatctt catttgttac tgctacaagc
    42661 atattagcag tgcttccata agggtcccaa gatgccttta tattgaacca agcatacaga
    42721 gatggtaaaa aagcaagacc taaaactatt acaagtacgg cataattgcc acgtatatct
    42781 tttaggtcac ttttaaaaat ttctaatata ttttttaaac cctttgaagg tcttaaattc
    42841 ttgatattat tccagtttaa aatttctttt aaactattag tttttttcaa atgtctcacc
    42901 tcctaaaata ttttgataaa gaaaaaagtt agatttataa tcaattttaa attatattac
    42961 tttttacaca aatagtaaag ttaaaatttt gtcttaattt taatacatat atattatata
    43021 taatagtatg ctataaatat atataaatat aggaaataaa aacaaataat ttataaaata
    43081 tatggtataa aaattaataa taaatcaaat atttataatt gatataatca attaaattag
    43141 ccattatgat atacttttgg taatgagaaa attctatgaa gggatgattg aatgaggtat
    43201 tttaaaaaat acataaaaaa atatatagta ttatttctaa cagcagtatt ttttttaaca
    43261 ttggaagctt tttgtgattt agctcaacca actataatgg caaaaataat agatataggg
    43321 gtttcaaata agaacttaga ctacatacta tcaacaggag ctataatgat aggtataact
    43381 tttttaggtg caatatttgc aataataaga agtgtaatct cggcaagagt ttctcagcat
    43441 ttttcttatg atttaagaca ggatgtattt gaaaagataa atacatactc attaaaacaa
    43501 atagataaat ttggtagagc ttcattaata acaagaatta ctaatgatat caatcaaatg
    43561 caacaattcg ttaatggaat gatgagagtt tttataaagt caccaataat gtgtatagga
    43621 agtttaatta tagctataaa attaaattta aagatgtcaa taattattct aattgcagtt
    43681 ttaattatat ttattataat attgcttaat atgaagatag ggtatagatt atttaaaaat
    43741 gtacaagaaa gtacagataa agtaaattca aaattaagac agtttttaag tggaataaaa
    43801 gtagttaaac tattttgtag atacgattat gagaaccaaa gctttgaaga gttaaatgat
    43861 gaacttttta aaaatagtag aaaagctaca actatgatgt cattttttag accgacaatt
    43921 acaataatca ttaatgtttc cataataata atattactaa ttggaaggca tttaatttat
    43981 tcaggagaaa ttaaaattgg gattatagtt gcttatgtaa actatatgac tagaatatta
    44041 acatctctta taatgatttc acatgttttt aatgtttttg ttagggcaaa agcatcttat
    44101 gagagaataa gtgaggtttt ggttgatgag aatgaagaag taattgagag agaagaaagt
    44161 ttagatgttg gagaaaaaga tttaaagttt gtaaactgtg atataatttt tgaaaatgta
    44221 gatttttcgt ataacaaaaa tacagatgaa aaaatattaa aaaacgtatc ctttaaaata
    44281 gaagaaggtc aaaatattgg tgtgattggt caaactggtt caggaaagac ttctttagta
    44341 aatttaattc aaaaattata tataattgat aaaggtgata ttaagattgg aggacgtagt
    44401 atatacagta tttctgaaga aagtttaagg gcaagaatag gtgtagtatt tcaaaatagt
    44461 agtgtgtttg gaaaaagtat attggagaat gtagttatgg gaaatagaga tatatgcaaa
    44521 gaggatgttg taaaatcatg tgaaatagca aatgcaaatc aatttataac aaaactcgaa
    44581 aatacttatg atgaagtttt attccaaagg ggtaaaaatt tatcaggagg acagagacaa
    44641 agaattacta tagctagagc tatagccaaa aaaccacaaa tattaattct tgatgattgt
    44701 tttagtgcaa tggatattaa tacagagaca attataagaa agaatataaa agagtatata
    44761 agtaatttaa gagaaaagga aagtaagaaa tgtactgtaa taactatttc acaaaagatt
    44821 agttctataa ttgactgtga taagatttta gtcttggatg agggtgaggt ggttggattt
    44881 gctacacatg aagatttact ggttagttct ccaatttata aaaaaatttt tgcgttgcag
    44941 aatttagtaa gtgaagatgt aaattaagga ggaagtatat tgaatttaag tactggaaat
    45001 cagaaggtca gtagattaaa gaaaaaagaa aagcctaaag attttaaaaa aacgtttaaa
    45061 agaattttta tgtactttaa aaaagataag aagattacta gatttatttt tttgctagta
    45121 ataattgatt caattatatc aactgtaata ccatatacta ttggaaaggt agttgacata
    45181 atagatatcg atacttattc aaaatccatt ttcttaaaag gaataattat tttactttca
    45241 ttttatataa ttgagttttc agttaaactg tctaagggga tatgtacagc tagattatct
    45301 caaagtatag ttaaagaaat gagaagtaat ttatttttaa aatttaaaaa tctaccaata
    45361 accttttttg ataaaagttc tactggagat attatgagta gagtgacaaa tgatgttgat
    45421 aatatcagta caggaatatc aaattcatta gtacagttaa ttacaggaat cttaaacata
    45481 ttgatgactt ttattatgat gtttgtatta agcccaattt taaccttttg cagtgtaata
    45541 ttaataccga tagtaatgtt tgtgagtaat tttattgcaa agagaactag agtttttttt
    45601 aagcaacaac aagttgagtt gggaaaacta aatgcacata ttgaagaaat gatatcaaac
    45661 ataaatgtag taaaatcttt taattatgaa agaaaatctg taaatgattt taaagaagtc
    45721 aatgatagaa ttctaaatat ttcccttaaa tcgcaaattt acacttctct tttaatgcct
    45781 attatgaatg tgattagtaa tataggattt gcaactatat gtattatagg aggcattctt
    45841 gcatctaaag atattataac cataggggtt atagctagtt ttttaagtta tgtaagacaa
    45901 tttacaagac ctttaaatga attagcaaat ctatttaata cattactttc agccatagct
    45961 ggatgtgaaa gagtttttga aattatagat gaacaagaag agcaagaagt gcataacatg
    46021 tttaaaagtg agattaacga taataaagat ggtaatttaa actctcattt tgtaaaggga
    46081 gaaattgagc ttaaagatgt aagtttttct tatgattcaa aaaagaaaat tttagataat
    46141 ataaacctaa aaatcaaagc tggcacatca aatgccatca taggagagac gggaagtgga
    46201 aaaacaacta ttataaatct aattacaaat ttttatcata tagatgaagg aagtatactt
    46261 ttagatggta aggatatata ctctattaac agaaactatt tgagaacttg ttttggagta
    46321 gttccacaag aaggttatat attcaatgat acagttatgg aaaatataag gtatggaaat
    46381 ctggaggcta ctgatgaaga agttatatat gctagtaaaa tagcaaatgc acataaattt
    46441 atagttaaaa tgcgtgaagg atatagtact gtattatcgg ataggggtga agatttaagt
    46501 gaaggtcaaa aacaattaat aagtattgct agagcagtat taaaaaatgc tccaatactt
    46561 attttggatg aggctacaag taacatagat atagcaacag aagctaaaat tcaagaagca
    46621 ataaataatt taacttatgg aaagacaagt ataattatag ctcatagatt aagtaccata
    46681 tcaaattgtg ataatataat agttttagaa aatggtagaa ttgtggaatc aggaaatcat
    46741 aaagaattga taaaattaaa gggacattat tatagaaaca tattattgac acagggaaaa
    46801 taagtattta gaaaaaactt ttaaaaaaat tgtttaaagg tatatggtgc gggtatatat
    46861 agactaactt aataaataaa aaatttaatt tgatatataa aatttaggag gaaagtaatt
    46921 atgaagaaat ttgtttgtac agtatgtgga tatatacatg aaggagatgc tgcaccagca
    46981 caatgtccag tatgtaaagt tggagctgat aaatttgaag aaatgaaagg cgaaaaaaat
    47041 tgggctgatg agcacagaat aggagtagct caaggtgtag atgaagaaat aatcgaagga
    47101 ttaagagcta actttgttgg tgagtgtaca gaagtaggaa tgtatttagc aatgagtaga
    47161 caagctgata gagaaggtta tccagaagta ggagaagctt ataagagaat agctcttgaa
    47221 gaagctgaac atgctgctaa atttgcagaa cttcttggag aagttgtagt tgcagataca
    47281 aaagaaaact taagagttag agttgatgct gagtatggtg caactgatgg aaaattaaaa
    47341 ttagctaaga aagcaaaaga attaggatta gatgctatac atgatacagt acatgaaatg
    47401 tgtaaagatg aagctagaca tggtaaagca tttttaggat tattaaatag atattttgga
    47461 aaataaattt aatataaagt agattttagg agtatctcac ttataatttt taaagttatg
    47521 agtgagatat tttttaatta tttgtacttt attttttatt tctaacgatt atatttttaa
    47581 taatagagaa taatttaata gtaataatgc acaactttta aaaataaatg attaatccaa
    47641 aaataaattt gattttagaa aagtaaattg acatgtaaag aagaatatat taaactaagt
    47701 ataaaaaatt tgctgttttg taaaggtggt ggataatctg ataaagtata tacataaaag
    47761 tgatgtagct attgttgttt tgcatgaaat ctatgggatt aatagcttta tagaagatgt
    47821 ttgtcaaaag tattataaat atggatatga tgtattttgt cctgaacttc tgggagaaaa
    47881 aagagttttt ccatattcaa aagtccaaga ggcatatgat ttttttataa ataatgttgg
    47941 ttttgatatt tataaaaaag ttgaaaagct tataaatgat ttaaaatcag agtataaata
    48001 tgtatttgtt gctgggtata gcgtgggagc aactattgca tggagatgca gtgaagattc
    48061 tttttgtgat ggcataattt gttgttatgg ctctcggatt agagattaca tggatgtaat
    48121 tccaaaatgt cctaccttat tagtatttgc aaagcacgat tcatttgatg ttgattctat
    48181 ttcttaccaa ctttctagga aacaaaatat ccaaattgag attttagatg caaatcatgg
    48241 ttttatagat agatattcaa ataattattc tgatattcaa acaaaagtat ttactattaa
    48301 agaaaaaagt tttctgtcta aatatttagc atgatttaga ttattataaa atatatagaa
    48361 agatttaatt aatctgaaaa gtttttatga cactattgat atttaaaata ttagtagtgt
    48421 tattatattt tttgaaataa aattaatcac tagaattata gtgttagttg tgcttttatg
    48481 acttataaga aactaatatt agatagtgta tttaaataga taataataaa ttcattatag
    48541 ttgaataaga atattatatg agaatattgc aatttgatat atttaccatt acaataaaag
    48601 tagttacaac tagaaaacga aataagttaa agtaagttgg caaattggag gtttttaaat
    48661 atgaaagcat ttaaaaagca cttcttgata ctttctttga tatttttact tattatatca
    48721 tgttttaaca atatactatg ctttgctgat gagtctgaaa attcaaaaaa aataagagtt
    48781 ggatattgtg atgactacgg aataatatcc tcatcaaaag agcattctta tacaggatat
    48841 ggttatgatt atctaagaga aatctcaaaa tatacaagat gggaatatga atttgtaaaa
    48901 gggtcttggg aagaatgttt agacagacta gaaaagggtg aaatagactt actcggacct
    48961 ttacagaaga atgatgaaag aaataaacta tttaattttc ctaaactaag ttcaggatat
    49021 gaatatagtg cactatatac aaaggatagt aacaataata tgttttatga agatataagt
    49081 tcctttaagg gaatgagagt agctgtactt agaggaaact ttcataatac tgcatttgag
    49141 aagtatagag aagaaaataa tttttcagtt gaatatatat actgtaattc tattgatgaa
    49201 ttaattgagt ccgtcaatga aaaaaaggca gatgcatttg tttgtggaag tattataaat
    49261 gctaaaggaa tgaaaatagt tagcaaattt tcagtagaac ctttttattt tgctactgca
    49321 aaagataaac ctaatttagt aaaagaatta gattatgctt taaaagaatt aaaaataaat
    49381 gatatgtatt atgagttgga attatataaa aaatatttca aaagagaggt caataattcc
    49441 atagcattta ctcggcaaga aatgaatttt attaaagcaa acccaaaact tactatggta
    49501 tatgattcag agtggtctcc tgtagagtat tatgatgaag aaagcaattc atttaaaggt
    49561 ataagttccg atttaatgtc aattatatca aaaaaatgtg gtataaaatt tgaatatatt
    49621 aaaacaaaaa attataatga gagtcttgat tacataaaaa gtggtaaggc aactatgcta
    49681 tgtggctcaa tcaatgaaaa tgataaggta aaacgattta atatgaaatt gacaaatcca
    49741 tacataaata ttccaatgat tatggtagga aaattggata ccaatctcaa taatgattta
    49801 aatattgctt taactagtag ttataagtct attgattctt atatagaaaa gagttttgaa
    49861 aatgctaaga caacatctta taagagtgta gaaagttgtc taaacgctat aaatgaaggt
    49921 aaagcaaact taatgatact tagttcttat cagtttgatg aacttttaag agaaggaaag
    49981 agtgagaatt tatctgttat atctgtttta gatacaagct atgggatgcg tataggggta
    50041 tcaaataaaa ctgaccctat attagtgtct attttaaata aatcaataga caaaattact
    50101 gaagaagaat taagtgattg tatatattct aatactattg acaaacctta taaagtacca
    50161 tttggtgtga tttttaaaga atattcaata caaattattt cttttgtatg tattttattc
    50221 ttaattgcta ttaaatatat aatttataat aaaaagaaga aagaggatta tctaaggaag
    50281 atagcatata cagacccatt gacaggagca gatagtatag ataaatttaa aataaattct
    50341 aataaactgt ttgctaagaa taatccagaa gaatatgcgt tgttttatat agatgttgac
    50401 aaatttaaat atataaatga tatgtttgga tatgatatgg gaaatgatac tttgatacat
    50461 atatcaaata ctattgcaag tgaattaaag gaagatgaaa tttttgcaag agtttctgct
    50521 gaccattttg ttcttcttat taagtataag acagatgatg atattaaaac aaggttaaac
    50581 aatatttaca ataaggttca aatacttagt aatcctaaaa taaactatta taaattgatt
    50641 ttagattgtg gtatatataa aataagtaaa agtgataatg acataaatac tattatggat
    50701 agagctaata ctgcaagaaa aactataaaa ggtggtcata aaaattcatt tgctttttac
    50761 gataaggaaa tgcacaaaaa aatattaaaa gagaaagaaa ttgaaaattc aatggtagat
    50821 gctcttaata atggagaatt tattgtttat tttcaaccta aatatagcct ttcagattat
    50881 caaattatag gggcagaggc tcttgttcgt tgggataatc cacaaaaagg tctaatacca
    50941 ccaatagaat ttattcctgt atttgagagg aatggtttta ttgtaaatat tgatttctat
    51001 gtatttgagg aagtgtgtaa aaagattaga gaatggatgg atgaaggaca aaaagtagta
    51061 cctatatctg taaacttgtc tcgaatgcat tttgtaaata gtaattttat agaggagttt
    51121 aaattaattg tagataaata caaaattccg acaagactta tagaattaga gctaacagaa
    51181 acagcagtat tagataatat agaaggatta cttgatacaa tgaacaattt gaaagaaaag
    51241 ggatttgtaa tctctatgga cgattttggt acaggatatt cctctcttaa tcttttaaaa
    51301 gaattgcctg ttgacatatt aaaactagat agagcatttt ttacagaaaa agatgagagt
    51361 aataatgaaa aaattgttat atctaatgtc attaaaatgg caaaggaact gaaaatgaaa
    51421 gtaatatcag aaggtgtaga gactatttcg caagtagaat ttctaaagca aattgggtgt
    51481 gatatggtac aaggatattt gttttctaag ccaatgcctg taaaagagtt tgaaaaaatc
    51541 gcttttaaaa aagaataaag caatgtgtaa ataatgacat atataataaa ggtgctttaa
    51601 tattagttga gtatactatt ttagagcatc tttatttttg tattaatttt tacgatttta
    51661 ttaataaaat aaatcaaatg agaaaaaaaa tcaaaagaat acaataatca cttgcaaaaa
    51721 atatactatt gtgatagact taaatcaaaa ttaggaattg taaaaagaag gaaaaaatag
    51781 gatgttattt taatagatta agatttaagg tattgtaatt tatatataag ataacgattg
    51841 cttgtagatt taaattactt taagaggagg gatattatat gatgccattg atattatcaa
    51901 atacaggtga agaagtagtt ataaagaaaa tttcgggaag tgataagacg aggacttttt
    51961 taaatagtat aggttttgta gtgggtacaa atgttaaaat agtttctaag atagatggta
    52021 acttaataat agacgtaaaa gatactagag ttgcattaga taaaaaaatg gcaagtagaa
    52081 taataatata gggaggtaat gacatgaaca gactgaagga tattaagtgt ggagagactg
    52141 taaaagtcaa aaagttagag ggagaaggag ctacacgaag acgtataatg gatatgggga
    52201 ttacaagagg tgttaatata tttataagaa aagtagctcc tcttggtgac ccaattgagg
    52261 taacagtcag agattatgaa ctatcaattc gtaagtcaga tgcagaaaaa attattgtgg
    52321 aataataaag attaattaga gtagggttaa tgtaattata taatgagaat gattttctgt
    52381 atcacaaaca gtgatgtatt taaataataa tggagggaat ggaaatgtca ataaaaatag
    52441 gtctcatagg aaatcctaac tgtggtaaga caacaatgtt caatgggctt acaggctcat
    52501 cacaatatgt tggtaactgg ccaggagtaa cagtcgaaaa aaaaggtgga aaattaaaag
    52561 gaaataaaga tgtggaaata gtagatttac caggtattta ttctttatct ccatatacat
    52621 tagaagaagt agttacacgt aattttatgc tagatgataa gccggatgca gtaataaata
    52681 ttgtagatgc atcaaatata gagagaaatt tatatcttac aactcaagtt ttagagctag
    52741 gtataccgac cgttatagct cttaatatga tggatattgt aaataagaat ggtgataaga
    52801 taaatattaa agaactatct gaagttatag gttgcccagt ggtagaagta acagcagtta
    52861 aaggtcaagg aattatggag gctgctgaaa aggcagttga acttgcaagt agtaacaata
    52921 aattaaattt caaattaccc tttgttgatg aaagtaagga tgcaatagaa aaaattgaaa
    52981 aaataataga agaaaagact ccatatatag atgtagaaac acgctggtta gctataaagc
    53041 tatttgagag agatgaaaat gttatacaaa aacttgatat ttctaaaaca atattgaata
    53101 gtattgaaga aataactaga aattgtgaag atgaattgga tgatgatagt gaaagtataa
    53161 taacagccaa tcgttatgaa tttattagtt caataatttc aagtataata aagaagaatc
    53221 gaaaaggtaa ggaaactgta tcagataaga tagataagat tgtaacaaat cgtatactag
    53281 cattacctat ttttgcactt ataatgtggg gagtttacta tatagcagta agttcattgg
    53341 gtactatagc aactgattgg acaaatgatg tactgtttgg agaaattatt caaggcaatg
    53401 taagtaattt tctagcttct ttaaatgtag cagagtggtt acaaggactt gtagttgatg
    53461 gtctaattgg tggagttgga gcagtactag gatttgtacc acaaatcatg cttttattct
    53521 tattattatc aatattagaa gattgtgggt acatgtcacg tgtagctttt ataatggata
    53581 gaattttccg taagtttggt ctttcaggaa aatcatttat accaatgctt ataagttcag
    53641 gttgtggagt tccaggagtt atgtcaactc gtactataga gaatgataga gatagaaaaa
    53701 tgactattat gttaactaca ttcattccat gtggagctaa gattccaata atagcattat
    53761 ttgcaggggc attatttgga ggtgcttcat gggtagctcc atcaatgtat ttcttaggta
    53821 ttgcaatgat aatcatttgt ggaattatat taaagaaaac tagtttattt gcaggagaac
    53881 catctccatt tgttatggaa ttacctcaat atcatattcc aagtgcaaaa ggtgttttaa
    53941 tacatatgtg ggatagagga aaagcattta taatcaaagc aggaacaatt atatttgttg
    54001 cttgtggagt aatctggttc ttacaatcat ttaattggtc attacaaatg gtagatgctg
    54061 gagatagtat attagctagc ttaggaaata tagttgcacc aatatttgct cctcttggat
    54121 ttggaaattg gcaatcatca gtagcaacag ttacaggtct agtagctaag gaaaatgtag
    54181 ttggaacatt tggagtttta tttggtatat cagatgcaac agagcaagac ccaacacttc
    54241 ttgcttcagt tgcaagtatg tttacagtag ctagtgcgtt tgcatttatg gcatttaata
    54301 tgctttgtgc accttgtttt gcagctattg gagccataaa aagagaaatg ggttcatgga
    54361 aatggacatg gattacatta ggattccaaa ctttaacagc gtatataatt gctcttctta
    54421 taaatcaagt tggaagttta gttttaggta ctggaggaag tattgctggg gcaataatat
    54481 ctatatttat agcagtagca gtagtctttg tagttttaac ttattcaaat aaaaatatga
    54541 aaaaagaaaa aatgggtaaa ttaagttata tgaaaaatta attcatataa ataaggagtg
    54601 atgaatatgg ctacatttat tatagcagct attgtagtag tattaatggc actagctgta
    54661 atgtatatgg ttaaaaattc aaaaaagaat ggtagcagtt gtggatgtgg ttgttctgga
    54721 tgctcaagta gtaagagttg tcatagcgct aagagataaa taggataaga gcctgataaa
    54781 aagttaaatt gctttattat caggttctta ttttttgtat aaataacaaa tttcatattt
    54841 gaagtaggtt agactatgct gtaattttac tgtataatta tatataaata tatgattagg
    54901 agggattatt gctaaattaa gcaatataag tgaatattat gtttaaattt gatgttacaa
    54961 acgagcttat gtgtatagca aggggaagtg gtaaattttt tgcaaaaaaa ggtgctatgg
    55021 tagcctttaa aggaaatttt aattttgaaa aattgctgtt aggtccaagt aatggcggtg
    55081 gattgggaag agcattatta ggtcatgttc agaggtcatt gacaggagaa caaatgccaa
    55141 taatggtggt tgaaggtgaa ggtgaaattt acctagctca aaatgcttac catgtagatg
    55201 taatatcaat tgacccaggt gattccattt atgttgagag tgaaaactta ttggcatttt
    55261 ctgaacaatt aaattacagt gtaaaattga ttggttcagg ggtaatatct caaaaaggtc
    55321 tttttactac acatttagta aataaaacag gtcaaaatca agatgttgct ataataacag
    55381 atggtaatcc tctaattctt gaaggacctt gttgtgttga cccagatgca ttagtagctt
    55441 ggactggaag agaaccatat cctaaagtat caaaactatc atggaagact tttattggtc
    55501 agacttcagg agaatcttat catatggaat ttgttgaacc tggacaaata gtcattatac
    55561 aaccatctga aagattgtct ggcttaaaaa cagattctac accttctgat agaatgagtt
    55621 caggtggatt aagacttggc atagattaaa tcttatgata tagcttaatt attatttaag
    55681 atattaaaaa ataaaaaggg tagcagggat agatatgtta aaaggagttt tatatgtttg
    55741 agaaaattca taaaaggctt aaaggatatt atattttttt aataatttta attttatgga
    55801 agatagtatg tgttttgggt atatggagta gctatattct gcctcctcct gaaatagtct
    55861 tagatacttt tataaaaatg atatatgacg gttctatttt cttaaatgtt tatgccagtg
    55921 taagaaggat attaataggt tttttaataa gttcattgat ggctatacca cttggagttt
    55981 tatttggagc aaataaaaaa atgtatgaat atttcaaacc actatttgaa tttatgcgaa
    56041 gtacacctcc actagctttg gtgccaatgc ttattttatg gtttggtata ggtgaagaat
    56101 ctaaaattat aattattatt ttggcatcct tttttccaat atttttaaat acattgaaag
    56161 gtataaaaaa ttgtgacaat aaattaatag aagtaggtaa atcttttaat ttatctaaaa
    56221 agagaatttt ttataaaata ataatcccta attctatgct ggatattgtt gttggactta
    56281 gtttagggct tgggtatagt tttagggcta taattggagc agagcttatt gcagcttctt
    56341 caggactagg atatttgatt tctgatggaa aagatatgtc tagaactgat gttgtattgg
    56401 taggaatttt tgttattgga tttttaggta tactttcaga ttatctattt tcaaagataa
    56461 taacaaaatt tagcaaaggg aagcaggttg atttaaatgg ttagaagtgg atttttgcta
    56521 gagaatatat ataagaaata tttagttgat aataaagagc atttagttct tgataatata
    56581 tctttaaata tatctagtga agagataact gttatattag gtgaaagtgg atgtgggaaa
    56641 acaacgctac ttagaatttt agcagggctt gaaaatgcta ctagtggaaa cgtatatttt
    56701 tttaataatg ataaaaagtg tactccaaaa gtaggaatgg tatttcaaga aagtagactt
    56761 atgccatggc tgaatgtaag tgaaaatata cttttacata ctgaaaaaga taatagaaat
    56821 aaagttgatt tagacaagta tttaaaaatg atgaagcttg aaaagtttaa aaactcatat
    56881 ccaaatgaat tatcaggagg aatggctcac agagtaagta tagcaagagc actttccttc
    56941 aatcccgata ttttgctgat ggacgaacca tttgcagcac tagattactt cacaagaaga
    57001 aaaatgcaaa aagaagttgt aaatatccat aaaaatacta aaaaaggtgt agtatttgta
    57061 actcacaata ttgaagaagc tatggaaata gcaaagaaaa taattgtttt tagtaaaaat
    57121 aaaaggatta aacaatttag tgtcgaagat gaatataata gagatttaac taaaaactat
    57181 tatataaact tgaaaaaaga aatattaagg gaattggggg agttttaatt gattaaaaga
    57241 aacaaaaaga ttttagcagt aatgacatct ttaataatgg tgttggttgg gactgtagga
    57301 tgttcaacaa caaatgataa gaaagatgaa cctgcaaaaa aagaggctag tttacctaag
    57361 gaaataaatt taacttatgt aaaatcacca cttaatgttc ctagtatatt agaaaaaaag
    57421 gatgatttat ttggaaagga atttaaaaag gacaatataa aagtcaattt ccatgaattg
    57481 acaacaggac cagagcaaac tcaagcatta gcagcaggtg agctagactt tttacatgca
    57541 ttaggaggaa cgtctgcaat aatagctgca tcaaatggtg ttggtcttaa aattacgaat
    57601 atttatagcc gttcaccaaa aggatatatg cttataacta ataatgataa tattaaatct
    57661 ataaaagact taaaaggtaa aaaaattgta ggtccaaaag ggactatact tcatcaactg
    57721 ttgattgctg cacttgctaa agaaggctat actatagatg atgttgaata tgtaaatatg
    57781 gatattccta gtgctgcttc tgcattagaa agtaaaagtg ctgatgtagc aatgcttgca
    57841 ggtccagtgg cattgaaaac gataaattca ggtgccaaaa tgattgtaaa tggagaagga
    57901 ttagtaagtg gaataatagt tactgctgta agtgatgact ttgctgaaaa atatcctgaa
    57961 ttagtaaaaa gatttatgaa ggttcatgaa gaaactttaa aatatatgaa tgaaaacaag
    58021 gatgaagtga tggatgttgt atctaaagaa gttggtttat ctttagatga aacaaaagaa
    58081 atgtattctt ggtatgactt tagttctaaa ataacggaca aggatataaa agaattagaa
    58141 gatacacagg aatttttgat gtctaatgga atgcaacaaa agaaaataaa tattaaagat
    58201 atgttgtaca atcaaaatta gaatacttta aaatattaaa gtggaaaata cacccttatt
    58261 tattatggaa actgtatggt aaaagagggt gtatttttat tattatacaa aaacacaatt
    58321 aaaaacagct tactatttta tctacactta tgtagtctag taattttctc attttcttac
    58381 ggtgaagtaa aaacttcgaa tatgtgtcct cggattgttt tgtgtcacca taaactttcc
    58441 aatatttaat catagaaact tcataattac tatatcccat aaatccaatc acatctttaa
    58501 gaggatatcc taaaaaaata cctatttcat caggaaaaac atcactctta agtttatcta
    58561 ctaaatgctc taaataagca ttaacattcg tattttgttt gtatcctaaa aactttaaaa
    58621 agtttactgt ttttttacat tttaattttt ctgataatgc ttttttattt acaaataaga
    58681 tttttaatcc ctttttttct ttatcaataa ctataaaatc tattttctta cacttatata
    58741 gatatttata aatatcatta attttttcct ctctatttac atcagtaaac gaaatattta
    58801 ttatttcagc aggtttagag cctaaaaaga ctggacctaa aacttctaaa agccatttta
    58861 tatatgatga ttcaatttta tttttacaac aaaattgtga attacaacaa gacataaaag
    58921 ataacctcct tcaaatgaaa attaatatca agtaattaga ttat
//
LOCUS       DAYASV010000009        57075 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYASV010000009 DAYASV010000000
VERSION     DAYASV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 57075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.009"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(162..1001)
                     /locus_tag="ACGI68_000607"
     CDS             complement(162..1001)
                     /locus_tag="ACGI68_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0137065.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1025..1783)
                     /locus_tag="ACGI68_000608"
     CDS             complement(1025..1783)
                     /locus_tag="ACGI68_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0137066.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1830..2303)
                     /locus_tag="ACGI68_000609"
     CDS             complement(1830..2303)
                     /locus_tag="ACGI68_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0137067.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2332..2748)
                     /locus_tag="ACGI68_000610"
     CDS             complement(2332..2748)
                     /locus_tag="ACGI68_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0137068.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIVINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2795..3847)
                     /locus_tag="ACGI68_000611"
     CDS             complement(2795..3847)
                     /locus_tag="ACGI68_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0137069.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4243..4923
                     /locus_tag="ACGI68_000612"
     CDS             4243..4923
                     /locus_tag="ACGI68_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFH0137070.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5056..5376)
                     /gene="sugE"
                     /locus_tag="ACGI68_000613"
     CDS             complement(5056..5376)
                     /gene="sugE"
                     /locus_tag="ACGI68_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFH0137071.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     regulatory      complement(5446..5552)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            5758..6585
                     /locus_tag="ACGI68_000614"
     CDS             5758..6585
                     /locus_tag="ACGI68_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFH0137072.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7407..8081
                     /locus_tag="ACGI68_000615"
     CDS             7407..8081
                     /locus_tag="ACGI68_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137073.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKDALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIYNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8069..9229
                     /locus_tag="ACGI68_000616"
     CDS             8069..9229
                     /locus_tag="ACGI68_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137074.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASVLTTFAYILLNNLYNSIQD
                     KYLKTPINTGIITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9466..9972
                     /locus_tag="ACGI68_000617"
     CDS             9466..9972
                     /locus_tag="ACGI68_000617"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFH0137075.1"
                     /translation="MTFSRIVFALLMLSSTFSSLPFWICYFWCGISDILDGLIARKLR
                     QQSGIGAKLDSIADFIFAVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYR
                     TFSSLHTLMNKITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELN
                     RDIKSIFM"
     gene            10258..10926
                     /locus_tag="ACGI68_000618"
     CDS             10258..10926
                     /locus_tag="ACGI68_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137076.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10929..13397
                     /locus_tag="ACGI68_000619"
     CDS             10929..13397
                     /locus_tag="ACGI68_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137077.1"
                     /translation="MKNYLSLSIKELKSQKFMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITIGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGHLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGTSENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTVAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTNVSLI
                     FSFISALPSAIYASHVSPKIAMVGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSTLDKIAKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRYTNLKPKDSITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNRSLY
                     KMFLWEGVYYGIFAAIFGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISVLAC
                     IIATLIPLRKVKKMNIIDCIDINL"
     gene            complement(13637..15061)
                     /locus_tag="ACGI68_000620"
     CDS             complement(13637..15061)
                     /locus_tag="ACGI68_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFH0137078.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNIALLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15080..15772)
                     /locus_tag="ACGI68_000621"
     CDS             complement(15080..15772)
                     /locus_tag="ACGI68_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137079.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15824..16603)
                     /locus_tag="ACGI68_000622"
     CDS             complement(15824..16603)
                     /locus_tag="ACGI68_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFH0137080.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLCMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSYE"
     gene            complement(16600..17370)
                     /locus_tag="ACGI68_000623"
     CDS             complement(16600..17370)
                     /locus_tag="ACGI68_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFH0137081.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQNLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17367..18071)
                     /locus_tag="ACGI68_000624"
     CDS             complement(17367..18071)
                     /locus_tag="ACGI68_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0137082.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18326..18838)
                     /locus_tag="ACGI68_000625"
     CDS             complement(18326..18838)
                     /locus_tag="ACGI68_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137083.1"
                     /translation="MKKISQEKISTHIQEIPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSDCKIIVGMEFETGCVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19164..19421
                     /locus_tag="ACGI68_000626"
     CDS             19164..19421
                     /locus_tag="ACGI68_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137084.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19564..20190
                     /locus_tag="ACGI68_000627"
     CDS             19564..20190
                     /locus_tag="ACGI68_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFH0137085.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20437..21216)
                     /locus_tag="ACGI68_000628"
     CDS             complement(20437..21216)
                     /locus_tag="ACGI68_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0137086.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNGCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21284..21745)
                     /locus_tag="ACGI68_000629"
     CDS             complement(21284..21745)
                     /locus_tag="ACGI68_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0137087.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21721..22398)
                     /locus_tag="ACGI68_000630"
     CDS             complement(21721..22398)
                     /locus_tag="ACGI68_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0137088.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22631..23017
                     /locus_tag="ACGI68_000631"
     CDS             22631..23017
                     /locus_tag="ACGI68_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0137089.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23020..24819
                     /locus_tag="ACGI68_000632"
     CDS             23020..24819
                     /locus_tag="ACGI68_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HFH0137090.1"
                     /translation="MYAPFFIRFLLSTLTLSILIAVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25174..27048)
                     /locus_tag="ACGI68_000633"
     CDS             complement(25174..27048)
                     /locus_tag="ACGI68_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFH0137091.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLASIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDIA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27053..28717)
                     /locus_tag="ACGI68_000634"
     CDS             complement(27053..28717)
                     /locus_tag="ACGI68_000634"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFH0137092.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKDLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKKSKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDEGYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28731..29552)
                     /locus_tag="ACGI68_000635"
     CDS             complement(28731..29552)
                     /locus_tag="ACGI68_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0137093.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKNREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKDILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDIVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29651..30415)
                     /locus_tag="ACGI68_000636"
     CDS             complement(29651..30415)
                     /locus_tag="ACGI68_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137094.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVINDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30412..31161)
                     /locus_tag="ACGI68_000637"
     CDS             complement(30412..31161)
                     /locus_tag="ACGI68_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137095.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31665..32270)
                     /locus_tag="ACGI68_000638"
     CDS             complement(31665..32270)
                     /locus_tag="ACGI68_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0137096.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            32449..33087
                     /locus_tag="ACGI68_000639"
     CDS             32449..33087
                     /locus_tag="ACGI68_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0137097.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33219..33881
                     /locus_tag="ACGI68_000640"
     CDS             33219..33881
                     /locus_tag="ACGI68_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137098.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            33881..35062
                     /locus_tag="ACGI68_000641"
     CDS             33881..35062
                     /locus_tag="ACGI68_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0137099.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35243..37903)
                     /locus_tag="ACGI68_000642"
     CDS             complement(35243..37903)
                     /locus_tag="ACGI68_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0137100.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37900..38583)
                     /locus_tag="ACGI68_000643"
     CDS             complement(37900..38583)
                     /locus_tag="ACGI68_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137101.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(38927..39880)
                     /gene="blaCDD"
                     /locus_tag="ACGI68_000644"
     CDS             complement(38927..39880)
                     /gene="blaCDD"
                     /locus_tag="ACGI68_000644"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFH0137102.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40421..42535)
                     /locus_tag="ACGI68_000645"
     CDS             complement(40421..42535)
                     /locus_tag="ACGI68_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFH0137103.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REKYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(42793..43653)
                     /locus_tag="ACGI68_000646"
     CDS             complement(42793..43653)
                     /locus_tag="ACGI68_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137104.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     CVEERDNRQN"
     gene            complement(44248..44610)
                     /locus_tag="ACGI68_000647"
     CDS             complement(44248..44610)
                     /locus_tag="ACGI68_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0137105.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44844..45308)
                     /locus_tag="ACGI68_000648"
     CDS             complement(44844..45308)
                     /locus_tag="ACGI68_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0137106.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLAKLDVEVGFPVATYIDGKNEILQRIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45737..47158)
                     /gene="nhaC"
                     /locus_tag="ACGI68_000649"
     CDS             complement(45737..47158)
                     /gene="nhaC"
                     /locus_tag="ACGI68_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFH0137107.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47188..48252)
                     /gene="orr"
                     /locus_tag="ACGI68_000650"
     CDS             complement(47188..48252)
                     /gene="orr"
                     /locus_tag="ACGI68_000650"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HFH0137108.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFSNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48237..49646)
                     /locus_tag="ACGI68_000651"
     CDS             complement(48237..49646)
                     /locus_tag="ACGI68_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HFH0137109.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49646..51850)
                     /gene="oraE"
                     /locus_tag="ACGI68_000652"
     CDS             complement(49646..51850)
                     /gene="oraE"
                     /locus_tag="ACGI68_000652"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HFH0137110.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51844..52218)
                     /locus_tag="ACGI68_000653"
     CDS             complement(51844..52218)
                     /locus_tag="ACGI68_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HFH0137111.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52296..53726)
                     /gene="ortB"
                     /locus_tag="ACGI68_000654"
     CDS             complement(52296..53726)
                     /gene="ortB"
                     /locus_tag="ACGI68_000654"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HFH0137112.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSYFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53719..54027)
                     /gene="ortA"
                     /locus_tag="ACGI68_000655"
     CDS             complement(53719..54027)
                     /gene="ortA"
                     /locus_tag="ACGI68_000655"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HFH0137113.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(54171..55238)
                     /gene="ord"
                     /locus_tag="ACGI68_000656"
     CDS             complement(54171..55238)
                     /gene="ord"
                     /locus_tag="ACGI68_000656"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HFH0137114.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     NVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKIIFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMEEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55494..56867)
                     /locus_tag="ACGI68_000657"
     CDS             complement(55494..56867)
                     /locus_tag="ACGI68_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0137115.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGREITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRKNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        19357 a         9704 c         6755 g        21259 t
ORIGIN      
        1 actttttaaa atattaaata ctacatatag cttacataag tttaaaattt aatctattat
       61 aaaattaccc taattgccaa actttttaat ataaattttc caacatccta caaatataat
      121 caatagatat agaatcctaa cttacaccaa tatatattta tttaaaatag tcctaaaata
      181 cctgtaatag ctcccactac tatcagtata aacattattt ttattggaga aagacctttt
      241 tttaatgcaa agagtactaa taaagtaagt accaaaggaa ttaatcctgg aaatatctta
      301 tcaagtaaat ccgtttgaag actaaattta aaatcaccac tagtataact tattggtgta
      361 gataaattga taaacctacc tacaagcccc cccatgacta aggtacctag tatagaagcc
      421 ccttctataa ccttatttac tattccacct tgaagtatac tttcaacagc ttttttccca
      481 tacttatatc catttgtcca cattccaaaa gaaattgaag tcattattat atattgagct
      541 attacaaata atataggtcc tgcaattgat gctcctccct cagttaaacc aatacaaaca
      601 gctaacacta taggagttat aactccttga gttaaagtat ctccaattcc cgctagtgga
      661 cccatcaagc ctgttttgat agcattcata ccatctccac ttatttcatc tgctccatta
      721 gctttttctt cttccatagc tattgttatt ccatgtataa cacatccaca tataggttct
      781 gtgttaaaaa atgctagatg agtctccact tcttgtttta actcttcctt atctggatat
      841 aattttttaa gtactggcaa cattgaatgt gaaaaagctg ttgattgcaa tctttcataa
      901 ttgtaattag attgtgcaaa gaaaaaccat cttaaccatg attttattac atctttcttg
      961 cttaacgttt tcttagatac ttcatttttt atatctgaca taatattttc cccctcacat
     1021 gtatttaaga tgtagcacta tcttttccgt acttaattga agtaattgta agagcaagta
     1081 ttactccaat aacagatatt actattatat ctactttaaa ataaacagcc attagtattc
     1141 ctaatatgaa aaagggcata gtaaacttat ttccaatcaa ttttaaattc atagcaatcc
     1201 caagtgctgg caacatagcc ccaattacat taaatacatg taacacatta tcattcatta
     1261 cttccaacat accatctatc gcatttggac caaaataaac aactaataat gctggtataa
     1321 aactcattgc aaataacagt atctgtggtg gtactatatt tataaatgct actctatcaa
     1381 cattcccttt ttgaacctct ctatcagccc aatgtgcaaa aaaagaattt actgtcatct
     1441 ttccaatcca aagtacagtt gcaacagttc ccaatggtac agctattgcc attgcagtac
     1501 ttacatctag cttagatgct atagctaaag cagtacctac atatcctgca aaagctgggt
     1561 cccccatctg agctccccct gctgatataa aacctagata tatcatattt atacctgctc
     1621 ctataattgc tccttgaaca gggtctccta atataattcc cgttacaaat ccagccaata
     1681 gaggtctatt ccatgtaaaa aatccaacat aagtcaacca cggactccaa gaaagataat
     1741 aaaaaattgc tattaatact gcttgaatta gtgaaatgtg catattcttc ctccctattt
     1801 tcttttaaag atttacaaga tagtcttctt tacaataatg ttcctacatc aattttcttt
     1861 gcatctggta ctatttgtat aaagcattta acatcttttg atattagctc tctaaatatt
     1921 tctttttctt catctgacac agatatattt ttataaaaac tttttctacc aagccctgct
     1981 cccatacctc caacattcaa ttcctttaaa tctaccccac tatttactaa acctaagaca
     2041 gttttagggt atttgaccaa tactattaaa ttttcatctc cattatgtga ttttaagact
     2101 tcagcacctg attctacatc atgtacttca accttaacag ttgatggagc tgccatttgt
     2161 aaaacttgaa ctataaatgg gtcctttact actaaatcat ccacgattac tatccttttt
     2221 gctgatgtaa tcttacacca agcagtaata acttgcccat gtattaatct atcatcaatt
     2281 cttgttaatg ctatattact cataattatt cctccagatt tttaatttat tttacaactt
     2341 taaaacagtt ttaacatctt tgattcctga tatcccaact tcctcacata aattcttcaa
     2401 gtcttttaac ttcatagtat ctctgtttaa aaataactct aaaagcatag gtaaatttac
     2461 acctgtcaaa cattctaact cacatacctt agcaaattta tttattacaa tagcagatgt
     2521 attaaatgga gttccgccat aaaaatcaag caatactaaa accccttcat ccttatattt
     2581 ctcaattcca tcttctaact ttctactaaa ctcatctatg ctgtcaccat ggtttaatcc
     2641 taaagtttct acattttcct gtttgcctac tattaattca gcactttcta acattgcttt
     2701 agccatattt ccatgtgtgg ctataattat tcctgccata tgaatcatac ctttcttaac
     2761 cttttttata tggattatta tttagtactc taatctaatt atctatatta atcattatct
     2821 taccaaagaa aatatccctt ttgtatactt tttcaaaata agctggtact tcttctaaat
     2881 ttattctatg tgttactaat ttttcaacat tcatatcacc cttttgcata tgatacaatc
     2941 cagcactcca ttcttttcct gggaatggaa aggagtttcc aaaccaagtt ccctttacag
     3001 ttaattcact tcttactatc ttttcaaatt gctctctagt caaagctaca tccccatatg
     3061 gtacacctgc atacaatact gtacctccct ttttagctag caacagtact tgaccacatg
     3121 tcagtggagt accagccgat tcaataacaa tatctgcacc atctccatca gttaaccttt
     3181 ttatttcttc tacaatattc ttttcttttg catttataca aatatctgcc cctaactctt
     3241 ttgccaagtc taacttctca tcaaatacat ccacagctat tatttttgta gaaccaaata
     3301 tttttgccca ttgtattgaa aataatccta ttggacctgt accaagtact acaacagtat
     3361 ctccaacttt agcttctgac ctaaaaagac catgtcctgc tatacatact ggttctagcg
     3421 cagcggcagt ctcataactt atctcatctg gtatttttat taaatttctc tctttcacct
     3481 ttgtatactc tgcaaaacaa cctccaagtt ctttatttcc tattattgct acattattac
     3541 atcttgaata caagcctttt ttacattcat cacattcaaa acatggcata gctggacaaa
     3601 cagcaactct atctcctatt ttaaaactcc ttacttcttt accaacctgt gccacttctc
     3661 cagaaaattc atgccctaaa atttctccaa ccatatgtgg acctgtcttt gaatattttg
     3721 atatatcaga accacatatt ccagctactt ttactttaat tattacatca tctttttcta
     3781 atatttttgg tacatccaca tcctccactc ttgtgtctct tataccatag aatctaactg
     3841 acttcactat taaccacccc tactttttat ttgagaatat atccctattg tttatatgtt
     3901 aaatatctaa agcaactttc atgccaatat atgtaagcat tgtattcatg cattttataa
     3961 aattatcatt aaatgtgtgt caaagtgtgt caaaagtttc ttatattaaa acttatgtgt
     4021 gtcaatataa acgacactaa cattttatat tagttctctt attataagtt ttataaaatg
     4081 tacttttttt gttattttta taaaattgtt cggaaatttc ccttctattt gtgatataat
     4141 attaaatata aggtatcaat ataaattttc atgcgaatat cagaactttt ttgacactaa
     4201 tgccttattc aatataataa tttaactttt tggaggttgt ttatgaaaaa agtgtatcaa
     4261 ggaaagacta aagatgtttt tgaattagat aatggaaatt acttacttaa atttaaagac
     4321 gatgtaactg gtaaagatgg agtatttgac ccaggcgaaa atagtgtagg tctaagtata
     4381 gatggaattg gtagagctaa tttagagaca tctgttaagt tctttgaaat tctaaacaat
     4441 gctggaatta aaactcatta tgtaagtgct aatttagaag aagcaactat ggaagttcta
     4501 ccagcaaaag tttttggtaa tggattagaa gttatttgtc gttatagagc tgtaggtagt
     4561 ttcttacgtc gttatggttc atgtgttgaa gaaggtgcta aactagattg ttacgtagaa
     4621 gcaactttaa aagatgatga ccgttgtgac cctcttataa catctgaagg tttagaagct
     4681 ttaaacatta tgactcaagc tcaatttgat tcaatgaaaa gcatgactca aaaaatttct
     4741 aacatagttc gtgatactat agctgaaaaa ggtcttgagc tttatgatat taaatttgaa
     4801 tttggattct acaatgatga agtaatctta atagatgaaa tagcatctgg aaatatgcgt
     4861 gtttataaag atggtgtaat agttgaccct atggatttaa ctgctctttt attagataaa
     4921 tagtatatac atataattta caaatataat aaacttataa ataagataag ctatgaagta
     4981 aagacttttt aaatgttgat tatttttaaa cattttctcg cctttaattt catagcttat
     5041 ttaaattaat atactctaat tagtagataa tagctttaac cctacaattc ccaccacaat
     5101 aaaacctata catgcaattc ttgatatact aataggctca tttaaaatag ccatgcctac
     5161 aatagctgta ccgacggtac caattccagt ccatattgca tatgaagttc caagtggaag
     5221 atgttttagt gctaatgaca aaaaataaaa acttacaatc attccaacaa cagtaagtat
     5281 acttggagtt aatttagaaa atccatgtga attttttagt tgaaaagccc aaaacacttc
     5341 aaacaaacct gcaacaaaca aaaataacca tttcataaat tcatccctct tttcaaatat
     5401 gtctcttatt tttccaataa aataacctga gaaatattat aaaatatctc ccaggttttt
     5461 gtccttccgt gttcacaaca aaaactatgt tgtgggtttt atctcggacc agaccaatca
     5521 ttaataattg cggaacccta taaaactcta ttaattattc tattttatct ataataatac
     5581 tatcaacata tataaaaaaa gtcaatatat gggttccata tataataaaa ataactttta
     5641 tatacctttt aatattctta ttcacttctc tccataccca tttgggtcaa tctaagaata
     5701 tctaattttt atttaatatg ttataataac ataaaaatat tgaaaggagt aatttctatg
     5761 aatttctata aaattgcagt atgtcagatg attactacag aaaataaaat tgaaaatatc
     5821 aatcatgctg tagatatggt aacagaagct gccatcaatg gagctaaaat tgtagttttg
     5881 ccagaaatgt ttaactgccc atatgaaaat aaatattttc ctaaatttgc tgaagaatat
     5941 ccaggtgaaa caacaacgat attaagtaaa ttagctgaga aacatggaat ttatctagtt
     6001 tctggtagta ttcctgagtt agaagatgga aaaatctata atacttgcta tgtatttgat
     6061 aaaaatggag ctctaattgg aaaacatcgt aaaatgcact tatttgacat tgaagttaca
     6121 ggaaaagtta gttttaaaga atcagatact cttacagctg gaaatgatgt cactgtaata
     6181 gataccgaat atggaaaagt tggtatagct atttgctatg atattcgttt ccctgagtta
     6241 tcacgtttaa tggctcttaa aggtgctgaa atcgttatct tacctgctgc atttaatatg
     6301 actactggtc ccgcccattg ggagttatct ataagaatgc gtgctttaga taatcagata
     6361 ttctatgttg gtgctgcacc tgctagaaat atgaatgctt cttatatagc ttttggaaat
     6421 tcaagaattt cagacccttg gggaagaata attgctcaag ctgatgaaaa agaatgtatt
     6481 atttatgctg acattgatag agatttaatt cctgacataa gacaacagtt accacttctt
     6541 aagcacagaa gaactgattt atatgaactt aatactttga aataaaacag aacataaccc
     6601 cataaatcat aaattaagtg ttaaaaaaat ttatggggtt ctcattaatt tgatttaaaa
     6661 ttaatttcat aaaaataatg ctaatattaa tttaattaaa taatactaat aattactaat
     6721 gattatgcta attatcagca tttactaatt taatcactat tagttatttc ttttattaaa
     6781 tatctttact tttttactta ttatgtcttc tagctttaca tttggattat ggtatttctt
     6841 tctattttta gtcacatctt tctattgaat tacttgctta aaacaccaat gcacacatgc
     6901 cagataatgt tcacaattat ttagaaatct tgctctacct tcttcaatgt tgatattatc
     6961 tctcctagtt gatattatct ttcctaaaat ttcttttaat taaaaaacaa cattagttga
     7021 tatatcaagt atattttaag caactgaaaa aaacaataat aattgatata tcaactaatt
     7081 aatgtagatt aattcatttt tataatttat atacttttta attaatctac cttatttaat
     7141 taaaatcctt cctttctatt tatccatttt caacttaaat tataagattt catcagctat
     7201 atttttgcat accaataatt attaaatatc aaatatatat tctattaaaa tttctaataa
     7261 tttcatcctt gatttattta atatataaaa atatactaac tttaatgatt aagttttgaa
     7321 atataactaa gaatttgata atttattgag atttttatac taaaatatac atactataca
     7381 aacttaattt agaagggagc attcttatga acaataaaat actcgtcgta gatgatgagg
     7441 aaggtataat aacactctta aaagattact ttgaaatgaa tggttatgag gtttacactg
     7501 ctcaaagtgg aaaagacgca ttaaaaaaaa tatctaaaaa tccagatatc attctacttg
     7561 atataaacat gcctgaaata gatggaattg aagtttgtaa tagaattaga gattatgtat
     7621 catgccctat tctatttctt acagctagaa ttgaaaatac tgacaagata aatggttttc
     7681 gtgctggtgc tgatgattat attgtaaaac cttttgattt agacgaactt ggagctagag
     7741 tatctgccca tttaagacgt gaacaaagga agtcaaatca aactaaaatt aaattttata
     7801 atgatttaac aatagattac gaaaaaaggt taatttgtat atataataat cagatttcac
     7861 tttcaaaaaa ggaatttgat atagtagagc ttctctctat gaatcctgga caagtatttg
     7921 ataaagaaaa aatatatgat agagtctggg gatacaatag caatggaaat agtgatgtta
     7981 taatggaaca cattagaaaa atacgaatca aactatcaaa acatactctt gaaaattata
     8041 tagaaacagt ctggggagtt ggttataaat ggataaaata aataatatgt cattaaaaaa
     8101 agctttcttt gttcttactt tatcttcact aattatagca tcagtgctta ctactttcgc
     8161 atacatttta cttaataatc tatataattc tatacaagat aagtatttaa aaacacctat
     8221 aaatacaggt attataacca tagttcaaaa caatgtgaac caatttagtg attatgataa
     8281 aacattactt aatataatca acatacttca gcttgtactt ccacttatct tttttatagg
     8341 acttttatta ttagctgata taatttttta caaggtaaaa ttaaaaacac ccatagaaat
     8401 actcaataaa ggtgcaatgg aaatatccaa caacaattta gacttttgct tagaatataa
     8461 taataatgat gaattaggta atctatgtaa tgcttttgaa aaaatgaggt ctgaactaaa
     8521 taaaaacaac ataaagatgt ggactatgat taatgataga aaacaactta atgctgcttt
     8581 ttctcatgat ttaagaaatc ccctaacagt tctaaaaggt tactcaaact atttaacaaa
     8641 atacatacca acaggtaagc ttagtgatga gaagatttta tcaaccacac aacttatgtc
     8701 tgagcatatt aacagaattg aatactatgt agaaaatatg agtaatgctc aacgattaga
     8761 agatttagtt gtttcaaaat ctatgtcgaa tataaacaag tttatagaaa acttagatga
     8821 aaatatatct atcattgcta aacaagaagg aaaatctttt acacttaaaa gtcaaataaa
     8881 taatataaat ctattttttg atgaaaatat cattcttaga gtcgtcgaaa atataatatc
     8941 gaatgctttt agatatgcta gaaataatgt atccatactt atatatcttg aacaagaact
     9001 ccttaccttt gtaattgaag atgatggtat aggatttagt aaggaatctc taaaattagc
     9061 tttaagacct ttctatagag ataagacttt aaatgacagc aatgcacact ttggtatggg
     9121 tctttatatc tcaaaaatat tatgcgaaaa acatggtggc tctatttcta ttgaaaataa
     9181 ttctactaac agtgctaaaa ttactgctaa attctcaaca agaaattaaa atgattttgt
     9241 aagctttcac tgttcttata taaaatttgt tagtttagaa tttataaaat gtatttcaat
     9301 ttacaaccaa tatatatact attttgtaga agttgtaaga gttgcttcaa ttataatatt
     9361 tggcaaaagg catttgaaat aataatagaa atatatattt aaatatgtag aatagtatga
     9421 aattagaatt ttggaggtga ttatattaaa agttttgcta ataatattac attctctcgt
     9481 attgtgtttg cattgctaat gcttagttct acattctctt cactgccttt ctggatatgt
     9541 tatttttggt gtggaattag tgatatactt gatggtctta ttgcccgaaa gttgagacaa
     9601 caaagtggaa taggtgcaaa gttagatagt attgctgatt tcatttttgc ggttacaatt
     9661 ttggttactg tcggaaaaaa tattgctctt cccatgtatc tatggtggtg tattgttgca
     9721 atagcattgt tacggttgat tagttacagt attggttttt ttaagtatcg cacattttca
     9781 tcgcttcata ctttgatgaa taaaatcact ggaatttcta tctttgcttt tccattactc
     9841 tatatttgct ttggtcttga tgttgcagga attatgatat gtatcgtggc ttttctttct
     9901 tcttttgaag aaatagcaat tatgataaaa tcaaaagaat taaatcgtga tataaaaagt
     9961 atatttatgt gacaacttag aatttgtgac aaaaataatt tatataataa tgaggaagta
    10021 aaacataaaa aattatccac aagtttatgt gtaaaatagt tttaaagcaa tagagttgta
    10081 ttgtaaagcc tttagtgcat aaattacatt ttaaattaaa aatgatactg aaactgctta
    10141 tcagcactgc gaattatccg caaatagtga agacttttta gatttaagct aaaagttggg
    10201 aaaaaattga gttttaaaac ttacactctt cttatataga aaaggagagt gttttttatg
    10261 attataaaag caaaacaatt atcaaaaata tatggttcta ataacaataa agttatagca
    10321 cttaataatg taaatttaga aataaactca ggtgaatttg tttctgtaat tggaccttct
    10381 ggaagtggaa aaagcacact tttgcatatt ttaagtggat tagataatcc tacctctgga
    10441 caagtgttac ttgatgataa agatatatat aaacacactg aaaaagaact atctgcactt
    10501 agaagaaaat cctttggatt cgtctttcaa caatttaatc ttttacctgt tcttacagca
    10561 tctgaaaata tttcaatgcc tgtacttcta gataaaaaac aacctgataa agactattta
    10621 aatgaaattt catcattact tggaattgca gatagactca atcatcttcc ccatgaactt
    10681 tcaggcggtc agcaacaaag agttgcaatt gctagagcac ttattgcaaa accagatata
    10741 atatttgctg atgagcctac aggaaacttg gacagtaaaa gtggcagtga agttatgaat
    10801 cttcttataa aaacttcaaa acagtttgga aagactttag tggtaattac tcatgatgat
    10861 agaattgcaa aacttgctga tagaaaaatt tcaattattg atggtgttct tatggaggtg
    10921 aaatagaaat gaagaattac ctttcacttt caataaaaga attaaaaagc cagaaattta
    10981 tgactacatt catcattata gcaatagtac tatcaactat tatgacaact gtggttggac
    11041 agtcaattgg tatcctacaa aatttaagaa ttgaacaagc ccgttctttt aatggtgata
    11101 gacatgtatc ctttcaccaa ctcactaaaa atcaagttga tgacataaag aaagatgata
    11161 gagtttatca agctggtact tcaataacta ttggcagtag taaaataaag gattctggta
    11221 tctctgtact tgtaaaagag tatgataaaa caggactttc taactaccca aaattaatga
    11281 aattaaaatc tggacatttg cctaaagata aaaacgaaat agccttagat gaaaacacac
    11341 taaagcttat gggtataaaa ccaagactag gagtgactat acctatgaat ttagatattt
    11401 cacttttaaa tgataccata ccaccatata attacacagc taactttaaa ttgagtggca
    11461 ttttagagga tgattataca ggatatgtaa gtggtatagt aaatggtatt gtaggtaaag
    11521 gtacatcaga aaatctatta cctaagagat atattttatc ctctttggat tttaaaataa
    11581 aacagcaaga aaagtttcaa gaaatagtaa atcaattagc taaaaaaata aatctttctc
    11641 acaattcaat tcaatataat tggatttact taaatgctct taagatacag tttgaaaaag
    11701 atgaaaatag ttctaatagt gatggtatat ctatgattat attggtatct ttatttgttg
    11761 ccttattggt tttactggct tctggacttg taatttataa tattttaaaa atctctgtta
    11821 caaagaaaat taaagagtat ggatgtttaa gagcaatagg agctgaacca aaccagatat
    11881 ataagatagt tattttacaa atcttaattt tatgtactgt tgccatacct attggagctg
    11941 taattggaat tatatcttct aaaggcataa ctggaatggt cactaatatc ttaaatccag
    12001 atatacttct agcaaatgat aataaagaaa ttactgagct tatacataaa aatacaacag
    12061 catatatatt cccattagta ttaagcacta atgtatcttt gattttttca tttatttcag
    12121 cattaccatc agcaatatat gcttcacatg tctctcccaa aattgctatg gttggtagta
    12181 ctaccaaaat caaaagaaaa attaaaagag aaaaaacaat aaaaaacttt gaaagacatc
    12241 tagcatggct aaacttaaaa agaaataaag gaagaactat aataactata ctatcattat
    12301 ttatgagtat aacagtattc gttgcactga gtgagttctc taatgtatta gatgtatcta
    12361 ggtcagtaag taatttaaaa gaaggagatt tttccttaac aaatgaaata tctggttttg
    12421 ataaatcaac actagataaa atagctaaaa tgaaaaacgt caatcgcact tcctttataa
    12481 agtactctga atacaaacag ggtgaaatag atacagatat aaattttgaa aatagtggtg
    12541 agatgttaaa aatcattgga attgatgagc aaactttaaa agacttaatg ccttctatta
    12601 cagattctat actagaagac tttaaaaatg gttctatctg ttttataaaa aatccgattg
    12661 ctatttctac accaggtgta aaaactagat atacaaattt aaaacctaaa gatagtatca
    12721 caatcaacaa aaaacaacta gatatatata gtacagtaga taaaatgttc tttttacaag
    12781 gcaatggctg gataaatggt gttgatgtta ttgtatatga ttctgtttat aacactttga
    12841 caaataaaaa caagttcaac cagataaata tttatgctaa agataaatca aaattagaac
    12901 aaataagatt gtctattgaa cagatttgtg aaaataatcc tggctctcat tggatatcat
    12961 atattgaatc agataaacaa cttaaagaga gttttaaaca aatagaattt ctagcatggg
    13021 cagttatatt atttgtggga ttgattggta ctttaaacat cattaacaca actcatacaa
    13081 atataaatac taggactaat gagattggag ttaaaagagc tattggtatg agtaatagga
    13141 gcttatataa aatgttttta tgggaaggtg tttattatgg aatttttgct gctatatttg
    13201 gttcaattgc tgggtatgcc agtgctatta tcataaacat ggcaactata gaaaagcttg
    13261 atttcactaa tataccaatt acttcaattt tacaagctac tattatttct gtacttgcat
    13321 gtattatagc tactcttatt ccgttaagaa aagttaaaaa gatgaatata attgactgta
    13381 tagatatcaa tttataacta ttttacagat aataatataa attaaattat caatgactgt
    13441 ctctattttc tttcaaatct aactaataaa tctttttaac tataatataa gctttaaaaa
    13501 aggcctgtct caaaatagag ataagccttt tttcattgtc gaaataatga aaatatattt
    13561 aacttcacaa aaaaagagtg cctcacgaat taaaaatttc gctttgagac actctccttt
    13621 aatttaaaat atatagttaa atgctatgcc aaaatttcac acatgctcca cctgattttg
    13681 aattttctat tttaacagaa cctccaagct gttccactaa ttgtttgaca atgtaaagcc
    13741 caagaccaga atgtccacct tttgtctgcc tagcttcatc tcctcgataa aatttatcaa
    13801 gagccttttt taaatcttta gagctaaacc ctctaccaga atcacaaatc tcataagaaa
    13861 tacaattttt ttcatccttt acagtaatac taatattacc acctgatgga gtgtattgca
    13921 gactgttgga tatgatatta tcaaaaattc gtgtcaacct atcaacgtcg atttgaattg
    13981 gggattgtat attcccctgc attttcaata taagttcaac ttccttttga tgagcctgta
    14041 attcatagtc ttgcaccttt tgcctcaaaa attcaggtaa atttatagga acaagattta
    14101 attgtatatt tgacttttct aagtcagacg tatattgcat cttttggaca agagcagcac
    14161 ttttttctgt attttctctt atcactgtaa gatacctatg aagtttttca ttgtcatctg
    14221 tattattacc aataagtgca tctgtatatc ccaaaataat agaaagagga gattttaaat
    14281 catgtgctag agcttccacc atttcaacac gttcctgctc catcttccac tgtgcagata
    14341 aagagccttt taattcgtct ttcatttcag aaaaggcact acaaagttta ccgagttcat
    14401 tatctgaata atagtcgatt tcaaaatcaa ggtccttttc ttttatcttc ttggacgcat
    14461 caatcaatag atgtaatggt tgattgatat tctttacaaa gcgcttagaa agccatctag
    14521 taaaacccac gatatataga aatggagaaa acaaagccat tataataact gcaaatacaa
    14581 atctccctct aatatttgca aaggtgattt tcacttggta gaatagaata acagcaccct
    14641 caatctttcc attatcccct ttgattggaa cagtatggat ataaccacct ttacgaacag
    14701 ttttattgat aaaattatta aataattctt cttttgtttt aaatagtttt ttggggttag
    14761 taccatataa aatattgcca ttattatcca caacttgata tagcatatca tccccacgaa
    14821 tcgttctttt taaaccttct tcattcgatt gtgataacaa agctatgttt ttttcattga
    14881 tgtatttttc aattctagga acttgtcgct cataatagtt tggtggatag atatccttat
    14941 ttaaggaatg aatatacaaa tagagagcaa atacataagt aatcactgtg gcaactatac
    15001 tggctattat aataaaaata aaagtatgtc taaattgggt aataagggat tgatttttca
    15061 tttaataacc tctctctctt tacattttat tccacttata cccaataccc caaactgtgg
    15121 aaatatattc tttttctggt gtcacagcag aaaacttagc tcgtattttt tttactcgtt
    15181 caacaacagt tgaggaatca ccttcagaat cacaccccca tactttttca tagatttgtt
    15241 ctcttgagaa aacttgtcct gcatggagtg ctagtaattc aacaatatca tactctattt
    15301 ttgttaaatc aatattctta ccatcaattt taattgcacg ttccttcata tataagcata
    15361 acttcccaaa atacaaagta gatctcttgt tctcttcatt gatatactgt gaacgctttt
    15421 ccctacgcag attagcttca atccttgcca taagttcacg cagtccaaat ggctttgtta
    15481 cataatcatc ccctccaaag gtcaaccctt tgattttgtc ggtttctgat tgctttgcac
    15541 ttaaaaatat aattgggcat aaaacatcat ctctaattgc ctgacatacc tcaaatccat
    15601 tcattttagg catcataata tccagtataa taaggtcagg ctcctgtttt gataactcta
    15661 tacctgcctg accatcataa gctatcaaca cttcatgtcc ttttgccttc aattcatcct
    15721 caagtagcat tactaggtcc atttcatcat caataatcaa tattttactc aatataacac
    15781 cacctttctc ttatgggaag gtcagtatta aaacaatatt cgtctactca taacttttcc
    15841 ttccctccca cattttaaac caaataacgc ctcctatcaa gaatataatc aaacctattg
    15901 ctactactat aagctctata ctagctgttt gcatcacttt agagtaaaat gcctcagttg
    15961 aaatagttcc agcttcttta ataaaataaa ttcctggaag catacctagt tttactggcc
    16021 atgtccatgg aacaaatgcc catacttttt caccaacgct agttaatcca agtattgctg
    16081 ccatcaaaat tccaccaata ctaataccaa tagatgcacc cataccccat gcaaaactca
    16141 cccaaagatg aatagctaat agtggaagtg tcccaatcac cacaagtcct gctgctgaca
    16201 ggaataacca tattgttgct ccagatggca ctgcaatact cattcctatg caaagtatca
    16261 acgtagcaat aaatgtgcat atagttaggc aaaacagaag aaaaagaaat tttcccagat
    16321 ataatctgac acgagaaata cctacacata aaaaaccatt aaaatttcca gcaagctctt
    16381 cctcctgcac tataaatcct gctaaaatac ctactccaat aggaatgatg aatacagtcc
    16441 atactgtaaa aaatccttca aaagcaaatt cttgtgtact tccagattta gttgtgagat
    16501 aaaaaaccac acataatgaa aaaataatag gcatacaaag agtaagccac tggacagctg
    16561 tcctctttgc tcgaagccat tctgatgata atagttttat catttgtcat ccctcctatt
    16621 gaaccaagct gcagtaagtg ctgttaatac tataaacaca accaaagaaa caacaatccc
    16681 aataggaatc actgaagtat tcaaaagtgg gtctccagct tctaatatag taccgttagg
    16741 atgtatgttg atgattgggc ataccattct aactgcccaa ctccaaggta ctgcaatcca
    16801 aaatggtttt gtcgcagcaa gaacaccaaa tatcatacct aaagctccaa ctcccatact
    16861 taggaacata cctttccatg tagctagcca tagctgaagt ggaagtatag caagagaaac
    16921 aacccagcat acaatacttg cccctataat ctgtccaaaa ggaactggac cagctttcaa
    16981 tattaaacca gttataattg ttacgactat taaaactagc gttgaaataa aactgtagac
    17041 tgccatagct ataactttat taatccaaag tgtcatagga gacacattgt gtgtacgtaa
    17101 agcacgataa tttccagctt ttttttcctg gaaagctacc aaagtagcga acaatgcaaa
    17161 tccaataggc agaaataaaa atgaccacca gttaaaaatt aatgctaaaa gattttgcca
    17221 cgcccaagaa gttgtttgag ataatggatt ttgcatgagt gttgattgca ttataaatgc
    17281 atatgctgca aaagacactg ggaaaaaaac aataagttta cccataaatg ttcttttata
    17341 ctttaaaagt tctgattgta aagcattcat ctgttttgtc ctcctttaag aataacatct
    17401 gtaaaaaatt cttctagatt ttcattaggg tcaggcattc cttgaaataa tagtcgtcct
    17461 ccatttataa ttcctatatt atctacaact tgagcaacct ctgatagaat atggctagaa
    17521 agaattacag ttataccttt tttgggaaaa gaagcaatca attcacgcaa ttcttgaatg
    17581 ccaaaggggt caagaccatt ggttggttca tctagtatta acaactctgg gtcactcaaa
    17641 agtgcaatag ctattcctaa cctttgtttc atacccatag aaaattgtga agctctcttt
    17701 ttacctgtat ttttcaaatt tactatttcc aacacttcat aaattttatc tttaggtatt
    17761 cccaatattt ttgtatgtac taacaaattt tcctctgctg taaggtttcc atacaatgca
    17821 ggggattcta ttaaagaacc tatttttgca agagattctc gtttccaagg ctttctatca
    17881 aaaataatac gaccatttgt tgggcgaaga agaccaacaa gcattttaag tgttgtggac
    17941 tttcctgctc catttggtcc taaaagtcca tatatactat ttcgttcaat ttgtagagaa
    18001 acattgttaa ctgccatttg ttctccatat ctttttgata agttttttgt ttcaagaatt
    18061 aaatctttca tattaatttc tcccttcttg taatacctta aatctgttac taagagaata
    18121 ctaacatgaa aaaataacaa ttctataaca cctactaatt ttaataaaat ttaaaataaa
    18181 aaatagagtt accaatttta ttttggcaac tctatttaag tttatgtttt acagttatat
    18241 tagatttaaa cttagtttca ttatcacact aacttcaaat ttactctata atctttaaat
    18301 caaacactaa tttattaatc aaacactaat ctattaattt atatttcttt agacatctaa
    18361 tatagtttgc tactaaataa taattattta tatcatattt atctaatcct ttaaaagaag
    18421 ttatttcatc attaagctca tcactacctt ctacaaaaac acaacctgtt tcaaattcca
    18481 tacctacaat aatcttacag tcactataaa tttcctcttg agtatcgtac aatttttttg
    18541 catttccttc atttggaatt gtaaatacaa taggttttaa ttctttatca ctggaaatct
    18601 tttgtgaaaa aggcttgttt aacttaatat tattgataac tacatgttta aattcctctg
    18661 aagtttcttc tactattaca tattttcgct ttaaaaactc tattatttct aaaacagact
    18721 tagaatttac ttttaaaata cttttatatt tttcatataa agatttccac tgttcaattt
    18781 tttcctgtgg tatttcttgg atatgtgtac ttattttttc ttgagatatt tttttcaatt
    18841 aaactctcct ttcaatactt aaatatcctt atgaatctca catgactaaa gttacaaggt
    18901 ttctacttaa tagaattttg catacttaaa tatgtcttat taattctcta caggatatct
    18961 ccgtagttcc tacgattctt acataagtta cttattttaa attgttatta gttatattct
    19021 aaaaattatt tattttatca ctaatataat actcttaaaa actttacaaa tcaatttaaa
    19081 tttgctacct tttatttata acaacttata gtacatttct cctatgcttt tcaatatatt
    19141 acttttaaaa ggaggttttc attatgattt taaatagagc tgagcttttc cgaggatatt
    19201 ctttaaatag atttaacgag atatgcaata tattatcagt aaataaaatt aaatataagt
    19261 ataaagtgaa aaataatgct tactctaaaa aacctttttt taatgaaata acacttggca
    19321 atttaggcca aaaggaagat ttctcttatg agtacttaat ttatgttcat aaagacgatt
    19381 atgaacaggc agagtctcta ataaattcta agttgcgata gattatctat aaaacatgac
    19441 attctgttgt catgttttat atgttattat actttcaaat atgctactat attttcaaaa
    19501 ttgtgtgctt taaaactagc tttttttagt aatttaaagc ttaaaactaa ggagactcgt
    19561 tatatgaaaa taaatataaa atcaaagtta agtgaattta ttaaacagaa caatcttttt
    19621 gatgacagca gaataatatc ctatcttcct aatataacaa tagaaacaga caaaataaaa
    19681 actataatga ttaatgaggt tgtcccaact aacaccaatg acgattttta tagtgtagat
    19741 aaagatgctg attatttaaa aactactatt cctttatttg acagtgcagg aataaaagta
    19801 tctaacataa atgatatcct agatatgggt atctatataa ctaatgcagt aaaacttcct
    19861 aaatcagaat atacaataac tcgtgataca attaaattac atatgcctat tttagaagaa
    19921 gaaattaaat tatttaaaaa cctagaggta gtaatgttaa tgggagatgt tgcaaaaaaa
    19981 tcttttaata tgattactaa aaagcatata aaaaagaatg tcatcccttc catttcaaca
    20041 tataaacttc gtaataatga actttattat gataatttaa gagtatttcc atcatatatt
    20101 atgactggtg gaaacatact gattgaaaaa tcaaaatttg aaatggcatc agaagatata
    20161 aaaaaaatgt tcgagattat aaacggttga tattacaaaa taataaaaac taatataaaa
    20221 taaaaagtgc ctcaaaaact tctatttatg aggcactttt attttatatt agtgtaacta
    20281 tatactatta acataattta ttatttatat tgttcttttt aatctctatt ccatttagtc
    20341 taggtaacat tactctttac aagattactt tatatttctt ttatatctct aacgttttta
    20401 tttaataaaa caattattaa tacaagtcaa atatatctat ttatctaaaa atatatcttt
    20461 tacagtaata tctggatggt gatacttctt cctattcgtt aaaaaagatt tttttacatt
    20521 aatagcctct tttggacacc agtgaaaaca agctaggcat ctttcacact cattacccca
    20581 ctttggcttt ttatcaacta tacttatatt ctctacagga caaacccttt tacatgtccc
    20641 acatccatta catttatttt ctgcaaaaaa tttagtagtt gcaatttgca tattcttacc
    20701 tactaaacga agtacaacac tatctatatg tgactttaaa ttgttttttc cttctatatt
    20761 tccaatctcc aagttattga tacattttgc aatctcagct accctattag tctgattttt
    20821 caatctttct tcttcaacat ctggaggtgt tatcaaagca tttccaggca tattgattaa
    20881 aaaaccagca gataattttt tccccctttt tttaagtaac ttatctatag taaataaact
    20941 atgtccagct accccattac aagtggcaat ggcatatata tgagtattaa tattaaaatt
    21001 taatttctct acaaaagact ttactatatc aggaacatca gcaaaataaa caggaaaaac
    21061 aattaccaat atatcttcat ttatgtctat attattttct tttctatgta tagctaaggg
    21121 tataacttct ttacttccat ttaacgtttc ttttaaaata tttgaaacta ctagcgaatt
    21181 tcctgttcca gaaaaataat aaatcttact attcattctt atccctcttt ctatatttct
    21241 acatcatcaa tagttaaaaa gtctcttaaa ataggattta tatttacttt ccaattctat
    21301 ccaaaatatc agtaaacaaa ggcaattctt catcagagat agatttaaat gtctctgttg
    21361 catatatact tgcaatttca tttaatggtt cttctaattc tctacctaaa tctgttagga
    21421 atatttgaaa cttacgtcta tctttaacat ctactttacg gacaattaat cccttatttt
    21481 tcataacatc taaaattctt gttgtattag atttatcctt acatgatttt tcacataaat
    21541 ctatctgaga aatcccatct tgctcccata gaaaccttaa taacgcataa tggtcagttg
    21601 ttatatcata tccattttgt ttaaacatct tttttaaatc atcttttact tttaacgaag
    21661 tacttactaa cttagttgta gcttcatata gtttctcaaa atctttcatt ttacctccat
    21721 ttaaataatc ccttgctcct cccatctatc aattattccc ttatattgct caatatcttt
    21781 aaaagccatt ggctttaaaa cagcatttat agcaataaaa ttatagattt gagaaagcac
    21841 aggtggattt ttcttttcct ctatgcaata aataaatttt ttagatacct tttgtatctt
    21901 tgattctact ttttcagtta taggattttg attaaatcca gtttttatag ataaactacc
    21961 acaaacataa cctcccgaat ctaagaccat ggattttata aaagaaattg cttttgaacc
    22021 acgtgcattt tcatatgtag taactgctat acatggctta cgatacaata atctttcaag
    22081 catcatatgt gctctatctg taaacacctt gaaaagtcca gttacactac ttccataagt
    22141 tgggctacca aatataacac catcactctt agtaattata tcatgaatat actctacttt
    22201 atcattttga ttgatacact tgcccatttt ataacaattt aaacaaccaa tacaatatga
    22261 tggatttacc tcacttaaat caaaataatt aatttttaca tctggtatta atctttctat
    22321 atctttatac aagtaagtta aaacttttga tgtagctcca ttttttctag ggcttccatt
    22381 tataactgta attatcattt ctatatctcc tttttagttg ttatatcaac aataagtata
    22441 tcaactataa ttttagctgt caatataaac catttattta aagctatact tacacagctt
    22501 aaataaaagt tttatttaac tacatgtgct tatttaatca actataaatg aattattttt
    22561 aataattgac aaactaaatt aatgataata tactttatgt attacaaatg taagtagaag
    22621 gaggtatagt atgaaaaagt taccacatat ttcagaagca gaatatcaag ttatgaaaat
    22681 tatttggaaa tatgctccta tcagcactac tgaagtaatt gaaaagttgg tagaaacaag
    22741 tacatggagt cctaaaacaa ttcaaacaat gttattgaga ttagttaaaa aaggagcttt
    22801 aacatatgaa aaaaatagca gagtattcgt ttatactcca cttgttaaag aagaagaata
    22861 cgtagctaca gaaagtagtt cttttttaaa tcgtttttat aatggtactc ttaattctat
    22921 ggtactaaac tttttagaaa atgataaact atctgaagat gatattgagg aactgagaga
    22981 aatcttaaat aaaaggacga ctaaggggga taaataaata tgtatgcccc attctttata
    23041 cgatttttac tcagtacatt aaccttatca atattaattg ctgtaatttt acttgccaaa
    23101 aaagtattta aaaaacatat ttctgcaaag taccaataca atattggatt tatattgctt
    23161 ataatgttaa taattccatt tgtccctctt aaatatatta atcttggaaa tgtatttaac
    23221 tatttatata cttttaatca acttggaagc agtactaata ggacttctat gataaaaaat
    23281 agtccaaatt ctataattaa taactcaaat gcattaaacg atttttcagt atccctaaac
    23341 caagttaatt ttgatagttt aaatatttta ttactaatat catggataat tggaatttta
    23401 atcatgatag ctattactat tcattgcaac attaaaataa aaaatatgaa aaaatcaata
    23461 caaattttaa gagatgagga gatagtattt atatttgaaa agtgtaaaaa agatattggt
    23521 ataaataagg attttatact atgcaaatca cctattataa atacacctat tacatttgga
    23581 tactttaaac cacacattat attaccagat aagtctatat ctaaattatc tttaaaagat
    23641 ataaaatata tcttacttca tgaattacaa cactttaaaa ataaagatat actgataaat
    23701 tatataatgt gcttccttca aatattatat tggtttaatc ctcttgtatg gtatgcattt
    23761 aaagaaatgc gtatagaccg tgaaattgca tgtgatattt cagttttaaa aaagcttgat
    23821 aaagatagtc atatagaata tggacaaact ctaataaact ttgtggataa tatttcaaga
    23881 ccatcaaatc ttacacttac atcagatatt ggtgggtcaa aaaaacaaat aaaaaagcga
    23941 ctattgaaga taatggattt tcataatgaa tcaaatacgt taaaattcaa aagcatttta
    24001 atttttacat tgatagcttt aattgtattt ggaaatattc ccaaactatc tattatggca
    24061 gatatcaatg aaaaatatag acctacaaat gaacaaactg tgtacgagga cttaagtcag
    24121 tattttaaag gttttgatgg aagttttgtt atgtatgatt taaaatccaa gcaatatcat
    24181 atttacaata aagaaaaaag taaaacaaga atttctccaa attctactta taagatttat
    24241 agtgccttat tgggattgga aaatgggata ataacaagaa ataattcaca tatcccttgg
    24301 gatggaaagc atcattctaa tagttcttgg aatgaagacc aagacttatt ttctgctatg
    24361 gaaaactccg taaactggta ctttcagact ctagataaaa aaacagggct aaaaaacata
    24421 caaacttatc ttaggcaaat tggatatggc aattatgatg tttcaggtgg tatctcaaac
    24481 ttttggctag aatcttcatt aaaaatatca cctattgaac aggtagaatt acttgaaagt
    24541 ttttatacaa ataagtttaa atttgaagat aaaaatatag aaacagtaaa agattcattg
    24601 ttaatatcaa aaaaggatgg ggtatcactt tttggtaaaa caggcactgc caatataaat
    24661 ggaaaagatg taaatggttg gtttataggt tatgtggaaa aaaatgaaaa tacttatttc
    24721 tttgctacaa atattcaaag caaagattat tctaatggta gttcagcatc taagataaca
    24781 ttatccatat taaatgacaa aaaaatctat aatccttaat atatttactg tacattctaa
    24841 aaaattcaca taattttcat attaattatc aattaaattc atatacaagg cttattatat
    24901 aaacatagaa gatagtaaaa ctagcttaaa aacataatat aataccctat acaaatagtg
    24961 gcaactctcc ctcacatcct caattgttgc cactatttaa ttttttgtat gaagttaaat
    25021 atattttcat catttcaaca atttgggtgc aatacaatct ctattttgaa acaacctctt
    25081 taattgtttt agagtttatg aattatttct catactttaa aaagattgaa ttactcttga
    25141 taatcttctt ttgtagtcat tacatatcac ctactactca acacatattc tttgacctat
    25201 ttttacatcc ttgtttatat ccaaaatatt taccttctta ccatctggaa acaataccat
    25261 tgttaaagta gactttccta attcatgtat tctctttaaa tcaacattaa caagaacatc
    25321 tccctgttta accatatctc cttgtttaac agcaatatca aagccatctc catttaatgc
    25381 aactgtatca attccaacat gaattaatac ttctccatta gttcctttaa tcccaaatgc
    25441 atgtccactt gaaaccactg tttctaattt acctgaaata ggagaaacaa tttttccatc
    25501 tataatatca atagcaaaac catcacctaa cattttttta gaaaatgttt catctggtac
    25561 attttctaat gcaacaattt tccccttagc tggagacaca aagttttcat tattttcatt
    25621 aaacactaat tcttcgtctt catcttcatt tatagcttct ttaggcactc caaataaaca
    25681 tgttacaata aagcagaatg caaccacaaa tattgatgct aataagtatg tgattaaatt
    25741 atatccatca tagatataca ttaaataaga aggtaaaact gctaatccat atgaattaga
    25801 ttgaatatgt aaaattgata atattgctgc tcctaaacct gaagatgcac acataataat
    25861 caaaggcttt aaattatatc gaagctgaat cccaaataaa acaggctcac ttataccaaa
    25921 aagttgagat agcattgctc caactgcagt ttgtttaact tgattatttt tagatttttt
    25981 cataaatgct agacatgttc caatattcgc aaatccatac attgcacaaa gagtaataag
    26041 ggcattaaat cctgtatttg ctaatagtga tgtttcaatc attacataag tatgatgtaa
    26101 gccagtaata acagctaatg gatatgtaaa tccaataata aatcctccaa ttcctaaagg
    26161 caaatcaatc aataagctga tgatatcaac catttttaat tctattgtat gcattatagg
    26221 gcctacacct aacaaaataa ctaacattgt tattagcata acaaaaaatg gtgtgaaaat
    26281 aaggtctaat acattaggca tatgtttacg gaaccatttt tcaagattag ctccaataaa
    26341 tgctgtaaca atagctgtta aaacacttcc ttgacaacct acaataggaa taaatccaaa
    26401 tgccataatt gcttcaattt catttgatgg gtcagcaaca gaatatgcat taggaagtat
    26461 tggggaaact aacataagtc caataacaag tccaataaca ggtgttcctc caaatacttt
    26521 aaatgctgac catacaataa ttgctggtaa aaaagcaaat gctgtttctg taagaaccga
    26581 aacaagtgtc tgaatataca atggaatatt tgcagaagac attccaaata atccaagtac
    26641 attatcatta aacaaacaac cctttaatcc caagaataat cctgttgctg caataactgg
    26701 aataatagga acaaatatat ctgctagagt tctcataact ttcttaaatc cagtttcatt
    26761 atttttaact aattcatctt gttctttttt tgataaagtt tttaatccca ttttttcaac
    26821 ttctgcataa actttattaa ctattcctgt tcctaaaata atttgatact gtccagaagt
    26881 aaagaaaacc cctttaacct catcaacttt ttcaactttc ttatcattaa taatttctct
    26941 atctttaacc atcactctta atcttgtggc acaatgtgta atagaaataa tattatcttc
    27001 accaataatt ttaataagct catttgctat tttattatat ttattcattt ttctacctca
    27061 ctttcaataa tactgcatga aatggtttca aacgcactgt tttatttaat agatttatat
    27121 catctaaatt ttggaataca atatcatatt tttcttctac tcttatttct acaggtttat
    27181 cagaaaaatt ataataacaa tcaataacta ccaaatctaa ttgccttcta tacgcaataa
    27241 tatcatcact ttctatatca ataggttcta ttgaaccata aatcaaacaa tcagaatact
    27301 cactatcttg tctcaacttt atcatctttt tataaaattc taaaatactc tctgaattgt
    27361 taatctgact ctgtgcatta atatctttat aattttcaat aacctttaac catggtgttg
    27421 tatctgaaaa acctgcattt tctttatctg cccatggaaa tggcgttctt gcattatctc
    27481 ggcttcttaa attcacaaat tctaacgctt cttcttctga gtaaccttca tctattgaac
    27541 gatagtattg gtcaatgcta gataaatcat taaaatcatt tatggtatct cttttaaaat
    27601 ttgtcattcc taattcctgt ccctgataaa taaatggggt tcctcttaaa aagaaataca
    27661 ttgctcccaa catttttact gcatcatcat tcctttcgta ttgcaaaaga tactttgttg
    27721 tagctcttgg ttggtcatga ttttcaataa aattagctgc ccatccatgt tcctgaacat
    27781 acatttgact atctaaaatg cattttctta aatctggaat tgtccatgaa attcttttaa
    27841 accactcact accagaagca acatctaagt cagcatgttt aaagtcaaag accattgaaa
    27901 agaaaccatc ttctccaata aaatcaccta actggtcata gctaatacct gctgcttcag
    27961 caacagtcat acaatcatat tttttaaacg tattttcttt taattcataa agcatttttt
    28021 ctattccagg ctgatttcta actgtttttg tacatttaac aagaccatca acaccatcac
    28081 tttctaaact ttgaaaactt aaattctttt tgatgaatgt aatagcatca actctaaatc
    28141 ctgccactcc catttgagac caggtattaa tcatttggta aagttcactt cttaatattg
    28201 gattttccca attaaggtca ggttgttttt ttccaaaaga atgaaaataa tattcgtctc
    28261 tattctctac cttttcccaa acacttccac caaaaacaga cctccagtta tttggcaaat
    28321 cgtttccttt tttaaaaata taataatcat gataaggact ttctggattt tttaatgctt
    28381 cttgaaacca aggatgttca tcacttgtat gattaagaac taaatctaat ataattttga
    28441 ttcctctatc tttacttttt ttaattaact cttttaaatc ttccatactt ccaaattcac
    28501 ttgcaatatc caaataatca ctaatatcat acccattatc atccataggt gatttaaaaa
    28561 ttggacataa ccacaatgat gtaataccta aatcttttaa ataatctaaa cgagaaataa
    28621 ttccctgtaa atctccaatt ccatcattat tactatcttg aaagcttctt ggataaattt
    28681 gataaacgat ttctttttgc caccatttct tttccataaa ttactccttt ttatatgata
    28741 tttcttcttt ttaattcttt ataaattcca ttatcgaata tgtcctcaca tatagatgtc
    28801 gctatatctt taagttgttg atgactattt ttcattgcaa ttgtaacgtc tgatgcttga
    28861 aacatgacaa tgtcattttg accatcacca aaacatatgg tttctttttg agttactcct
    28921 aatctttctt gtaatctctt aattgctttg cccttatgaa aatctttttg aataatttca
    28981 taatactgac tagaaatatc tctctgtgct aattccattt tatcttgtag tatatctttc
    29041 acttcgtcaa ataccttttc atttgaccat aaacaaattt tatgtatatc ttgtgactta
    29101 tactcttcaa tgttattttc atatgttatt ttttcttgga tatgttgttt atttatacaa
    29161 gaattagttc ctttcaactg attcattgtt tcaaatattt ctttggcttt ttgattcatg
    29221 aaaacttttt cctgactctc aatcgaaaaa gcaacttctc tattttttaa taaacaaaca
    29281 acttccttta tcaatctttg attaaaagat tgattataaa gtaattcacc atgatattga
    29341 atgtaattac ctcctcctgc aatatatcca tcaactccta atgataaaac atcatcttgt
    29401 attgtaccca tacttcttcc agtacaaata acaacactgc aatgattctt ttgacataaa
    29461 cgtatagcat gctttgcaga ttctgggatt ccatatacct catctctaag cgtaccatct
    29521 atatcaagta ttaataattt gtacaatttc atcttccttt cttactatca aaatcataat
    29581 agcattcaaa aaaaaagata tcaatatttt gatatctttt aaaaaatatg ttcattttta
    29641 gcacaaattt tcattttcgg ggaatatgtt cactttctaa atacatcttg tatttaatat
    29701 aaagcagttc aattaaaata aaataaggtg ttgttacatc gtagtccagt tgtttatcta
    29761 cagaaataga tatagattga atatacaggt tttcatcaca taatcttgct aaaggattac
    29821 ttatcatttt tgtaatagaa accatataaa ttcctttcat tctcatttct ttagcaaaat
    29881 caataattgt agatgcatct ccattcaatg aaataaagaa aatcatatca tctgttttta
    29941 taaaatgatt aagtgcctga atcatatcat aaccataaac atcatatata attttccctg
    30001 ttggtaagaa aatccttttc atctctttag caactctttc ctgagaataa ccttctccaa
    30061 agacgattat acgatttgcc tttttcattc tttcaaacat aatcgaacaa tccttcttct
    30121 ctatatcatt tatcacctta ttatagcaat ccattaattg gtgtttactt tgatttttat
    30181 attctacttc tgtttcattt aagcgtataa taactcttaa ttcactatat ccaggtaatt
    30241 gtagtttttg agcaaaacga gaaagtgcac ttgtagatat atggtatttg tctgcaaatt
    30301 catctataga aagtttaata caatcttctt tatgattaag aatacactca cagatatact
    30361 tatcatttgc tgtaaatgaa ttataatatt tattgaaaag tccttcaatt ttcattcttt
    30421 tctccttcta tactctatgt aattcatatg aagcaaatct aatagagcat aaaatgctcc
    30481 tacgacttca taagatatag cttgtaatgt atcaagtttt tgagtttcaa catacagatt
    30541 atactcacaa agtgttgcaa ttgtattatt atcaagccta gttaaagaaa tcgtatgtat
    30601 atgattgttg ataaattttg caatttcaat tcctgctttt gtttctccag acaaagaaat
    30661 gataataaat aaatcacttt tttgaaatga ttctctcata aattcaattt cacctatatc
    30721 aaataagtca ataacacatt tacctactga aagaaatatt ctctttattt cttgagctaa
    30781 acttttctgt tcattacctg tcccataaat ataaattgtt tctgcatcat atattatttt
    30841 acaaatatct tgaaatgata ttttttgtaa ttcattcatt aatagtcgat aattctcgca
    30901 aacatcatga aaattattat cattattaac aaattctagt tgattatttt taagtaaata
    30961 ctttaaatca gagaatgaat ctaatccaat ttttctacaa agccttaata aagtagctct
    31021 agaaacatta cattcatttg caattttttc acacgaaaaa ttacggtatt ctctttgaca
    31081 aatcaatatt tgtttaataa taattttatc attttcattt aaattatcat aattttggtt
    31141 aactaacgat tctaatctca ttttttccac tccctaaata ttattttaat atacattagt
    31201 tgctttttct agggaagagt tttaaataat aaggataaaa taatcatttg attttatttt
    31261 tattaaaata aaaataacct gctaaaagca ggcaccaaaa agtacttatc ctttcgaaca
    31321 tgtactttag atattgcttt cttcggaagc ttcatctctt catactctaa aatagagttt
    31381 tttctaactc aaaagtattt tccatggtag aaattacttt aattcatctg ccatttattt
    31441 tttatcttat ttaataatta ctactacttg aatatttact tctttaagta aatttcatag
    31501 ttaacaaagc taaaattatt attatagatg ttaaaataca aaattgtaag tattgttata
    31561 aatatgctta cagctacaaa tatacatgat atattaaaaa gaagctgtaa attattatca
    31621 tacatacaag caactacgcc tctattaatc catgttaatt tttattattt aatacaagga
    31681 gttccatgag acatacatat ccattcatat tttaaattat ccaattctct tatagatttc
    31741 atcatctttt tataatccca attccatgga ttaggataag gaactatatt tccattctta
    31801 tttttgacta aatcacctgc aaataataca tcttcaaaaa tcatgcagac atgccctgga
    31861 gtatgaccag gagtatggat aatttttata ttcccaatct gcatattctc tccataaggt
    31921 tcaacatcct taatgagcct tctggaaatt gcctttccaa taaacttctt aaacccaggt
    31981 ctatccttct ctcctataat aaaaggtata tcctctacat gagcccatac ttttgctcct
    32041 gttttttctt tcaataattt aatattagat atatggtcaa catcatgatg agttaataaa
    32101 atatgcttaa tatctgtaag tttaatgcca agtgttttta attctttaat tatatttttc
    32161 cccataaaag gaagacctgt gtctataaga ataatttctt tatcaaacac aataaaacaa
    32221 tttgagccac gagtactatc taacattaat acattttctt ttatcttcat aaataaattc
    32281 cccttatttg gtattttacc tatataatta attaataaag ttataaatag ttataatact
    32341 tgatattaat tttatttttt attaaagtat actataggta tacaaatatt atatttacat
    32401 aaatatacca tcagtatact tttttatcaa tagaaaggaa taatttatat gtcagaatta
    32461 tcaaaaagag ataaagaaaa aatacaaaga gaaaatgaga ttattgataa ggcagaaaaa
    32521 ttattttgtc ttaatggatt tgataataca actatgaatg aattagcaaa agaagttgaa
    32581 tatactaaac gcaccatata caagtacttt tcatgtaaag aagatttatt ctttgctgtt
    32641 gtcttaagag gatataaaag actttgggat aatgttaaaa tagaaagtgc aaaaggcaaa
    32701 acagggtttg aaaaaataaa actttcatac tttgcttttc ataaatttta ctctgtagaa
    32761 ccttctctat tatgccttat gggaatgatt ggtattgtaa actctgaaaa atcggatact
    32821 gatatgctgt ttaaagaaaa gtttttttct tttaataaat ttatgtttga tgaaatacaa
    32881 ggaatgtttg aaattggaaa aaatgataga agtattaggc atgatattga aatacctact
    32941 cttatgtatt catcaatatt tacacttaca ggatttttta atttactatc tgtaacagga
    33001 aaatcatacc tgaacaactt taatatagat gaggaaaaat ttattgaaac aactcttgct
    33061 cttttaatag attcaattaa agcataaacc taagtttata aattaaagtt tatatactta
    33121 aatgataatt ttttctatct ctaatatgaa aaatattaaa cataaaacta ataacttagt
    33181 taatggaata actaaattac tgaaaggatt aaaagtaaat ggctaaaata cttcttttag
    33241 aagataatga tacaattaca tttggaataa aaacttccct tgaaaagaaa aatcacactg
    33301 tatgttgtta taatacgatt gctgaagcta aaaaaaattt tgataaaact attgatttaa
    33361 ttctgcttga tttaaatcta cctgatggta ttggatacga gttttgccaa tatgttaaaa
    33421 gtatagaaga tactccagtg atatttttga cagtccgtga tgatgaaaaa gacattatac
    33481 gtggattaga tatgggtgca gatgactatg tggttaaacc gtttttgctc tcagtacttc
    33541 aatccagaat taatgcagtt cttagaagaa ctcattcaaa tactaacaat actattacat
    33601 gtgaaaatat aagcttgaat aaggttgaag tcaaagcata tttagataat gttgaagtac
    33661 ctttgacagc aggagaatat aaacttcttc taagtcttat ggaaaataaa aatagagcta
    33721 tgactcgtac aaagttacta gaaatgctct gggatgtaga tggtaatttc gtaaatgata
    33781 acactcttac tgttacaata aaacgtctta gagaaaaatt aaaaaatcca ccttgtataa
    33841 aaacattaag aggcatagga tatagaatgg aggacaacta gtgaaaactc caagaaatat
    33901 caacagtaca gttgttttta taatattctg cttaattgta acttttttat tgagtgtaat
    33961 tctaacaact ataacttctt ttaatgaata caatgtactg tctaaggctc ttggtgcatc
    34021 atatgagtac aatcctaagt ctacagaaag tatactaaaa tcactgaaaa attctagtga
    34081 taaggatttt aaaacaggaa atgaaatttt atcaaaacat ggatattcta aaagtacatt
    34141 ttggagtaaa aacttttttt attttctagt gatatcgttt ggtataattt caacaatggc
    34201 tacagttgta tatataatca aacatagaac aaataagcaa aaactgagta gaataaacaa
    34261 tcttactcaa tacttagaag aagtgaattt aggaaaagag tctgtacttt tacgatgtga
    34321 agatgaattt tctcaccttg aagatgagat atataaaaca gttggggaac ttcgttgttc
    34381 aaaagaattt gctttaaaag aaagacaaac tctttcagat aaccttgcag atattgctca
    34441 tcaacttaag actccaatta cctctatgtc acttatggca caattacttt cagaaaattg
    34501 tccagatgaa gaaatagatt atattaacag acttacaaat caaatctcaa gacttgagag
    34561 actagtttca tctttactta cattatcaaa attggatgca agtactttaa tatttgaaag
    34621 caaacttata gatgtacatt ctcttcttac ttatgcaatt gagcctatag aaggttcttt
    34681 aagagaaaaa catcagacat ttactatcga ggcttctcct tcggtagaat ttgaaatcga
    34741 tattaattgg actttagaag ctcttttaaa tattcttaaa aattgtagtg agcatataga
    34801 agatggtgga tatatagttg cttattatag tcaaaatcct ctatatgttg agattatcat
    34861 agaagataat ggtaaggggt ttgctcagga agaacttcca tatattttta atagatttta
    34921 tcgtggtaaa aatgctagta aggacagtat tggtataggg cttgctctat caaagtctat
    34981 tatagaacgt caaaatggta ctctacatgc agaaaattgc cataatggtg gtgcaagatt
    35041 cataattaaa ttttacacat aaaatgctgg attataggta aaatacctat aatccagcta
    35101 tatctatatt ctatgttatc tattgtttta gtaatatact tctatttaca tttaaggttt
    35161 tattaaacaa acaacttaac tatccctata cttgtaagta gtcttaaatc gaaaccttta
    35221 aataagttca aatagatttt gtttatattg tttcattttt tatagattct actatatttt
    35281 cattcttcaa ctgcttgtat cctgtcatat atgatgctat tacaataaaa agtatcaata
    35341 tagtaaaacc tattattgta gatataggtg caaatggtaa atactctata aaatatattt
    35401 catttatacg tagaaaagca tacactattc ctatttgcac aggaatacta agtagtaatg
    35461 gtgtaattcc taagaataaa ccttccaaaa taagcatctt ttttatgcct tctggagata
    35521 atccaacaga acgcaacata gcaaactctt gtcgtctact tctaatattt cctgacactg
    35581 tagcaagtac atttgataat ccaatcatag ccaataaacc tgtaataaag cctactatta
    35641 aattcatggt ctttattcca ctagcctttt gtttttcact ttctaaaaca tcctcaatac
    35701 tataatctcc tgaaccatag tctttattat aaatggactc taatttttct cttactctag
    35761 gaatgtcctc acgattattt actttaaata ctcctatcag cctatttgaa cgtaacctac
    35821 ttacttcatc ataattttta gatatttcct gcaccataga cattggaact atttgcatta
    35881 atgtatactt tgaactagta tctactattt taggaagtgt gcttgtaata gctcccactt
    35941 tcatgttgta agtataatcc cctttatctt cgtcatatac tttttcattt aatttaatgt
    36001 tatctcctat tgaaagattt aaataatcta tatatacttt atttctacgt gtactacgat
    36061 taatatcttc ctccttatta tatacaattg aggttggatt ttttgtatca taaaataact
    36121 ttgggtctac agaaatttgc ctacaatatt ctgtaaaact cttatcatca agcccaatta
    36181 tatttgaaat tatacgaaac ttgccatcac gttctatagg agagtacttt ttagatgaag
    36241 ctatattttt aaatcctcca tttttcgcaa gttcttttga agcatctttt tcagtcaacc
    36301 aagtggaagc agacattttt tgataatata gtgaactttt tacaccttct gtattatcta
    36361 atttttcaat gaaagcagac tctacaggtt cactattttc caaataaact gctatatctc
    36421 tttggtcctt ataattatca cttccataaa cagcttttgc tccttcttga tttgcaatta
    36481 tatgcaaaaa acatgtaagt aataaaaaag ataatgttaa agatattgtt gcagtacgat
    36541 aagatttttt acgagcacgt agtgcgtttg atgcaagttc tccttctatt ccaaatatct
    36601 ttcctaatga atatttatgc gactttttga ttttaacact atctccttgt cgtattgctt
    36661 ctataggaga tatctttgat acttttcgtg ctggtataag tgcagaaatc cagactgtta
    36721 ctattgtaag taaaactgct ggcagaaaag caggaatacc ataagtaaat accacttctg
    36781 gaacatcagt atgataatta actgaattaa cataaactat aagcctatta cacaataacc
    36841 atcctaaaaa taaacctatt ggtaatggta taattgtcag taaaaagcct tcaaacacta
    36901 cagacctttt aatttgtttt ggtgatgcac caacactaga taatatccct aattgagtaa
    36961 gtctagcatt tgcagacagt gaaaacgcgt tgtaaatcac cattacaaac acggctactg
    37021 taaaaattgc tattgtaaga tacataatag gagttgaaaa ttttgatata ctactcatag
    37081 atgctttctg ttctggtgaa aagactaaca tctttaaaag ataattacta ttgtatttaa
    37141 gattgtattt tccatattca ttagtcttcc atcccaatga tttagcaagt tttggaagtt
    37201 ccttatacgt gtctcttata tttttaaatc gcaagtacac agttatacta tcattaggtt
    37261 ttatacttgt tttatcaaga tatccaagag ctgtatatgc aggtattact gatgaagtag
    37321 aaacatctac ctttcctact attgtcaatg tagcctctga aattttttta aaactttccc
    37381 ctttcgattt tggggctatg ggttcaagag gctttccatt taggattcta tctcctattg
    37441 ggagagtaat tttatcacca atttttacac ttggattatt ctcaaaatat tgttttgata
    37501 atgcaatttc attagcatta tttggtcgac gaccctctat tatagtatcc ttttctggca
    37561 tagaattcca atactctgat gtagcatttc tataaattat ataatctctt ttctcatcat
    37621 taattttgcc tacataccat tgtcctttta ccattacatc atcaactgaa gaaaaatttt
    37681 caattaatgg taagtcactt ccataagtat agtcaaacaa ttcaccatgc cagttacctt
    37741 ctttctcaat tgaaagtttt ataaaatctg tccacatggt atacaacagt ccacacaaag
    37801 aagacatcat ggttgtagta agtaatatag ctaacattat agctatacta ctacgtttat
    37861 tgcgtcgtac aaagtctaag gtgtattctt gaagaatatt catttcttca ccaccttttc
    37921 agagacaatc actccatctt ccatagttac aatacgattt gcctctagtg ctattttctc
    37981 atcatgagta ataagaagta ttgtctgatt aaatcgttta tttgataagt ttaacaagtc
    38041 tacaatttct tctgaatttt ttctatcaag attaccagtt ggctcatctg ctaataaaat
    38101 agctggtcta tagattaaac ttctagctat agataccctt tgttgttgtc cacctgaaag
    38161 ttgacttgga agatggttta atctatcaga taatcccaat atagatacaa tctcagagaa
    38221 tctatcctca tccacctttc ttttatcaag taacatgggt aataatatat tttttctaac
    38281 atcaagggta ggtataagat tataaaattg ataaataaga cctacttttc tacgtctaaa
    38341 aactgcaaga gcttcttcct ttaaacttga tatttctgta tcttcaacat aaatctttcc
    38401 agatgtaggt ctatctactc ccccaagcag atgtaacaat gtagatttgc cagaaccaga
    38461 tgctcctaca atggatacaa aatcaccttt ttgaactgat aaatttacat tattaagggc
    38521 tgtcactctg gtttgatttg aaccatatat ttttgtgaga ttttcacatt ttaatatttc
    38581 catatacaat catctccttt atggttaaat tttagcaacc taaaatgaca ttatagtgac
    38641 atgaatacaa atataaatta ctattaaaaa tattgtttag tttttgcaca ttatttaaaa
    38701 ttataatata aaacaaaaac tatgcaatat attttattta tacttttaat atattacata
    38761 gtctttaaaa ggtattaact ttaaatctat aggtattcaa tttaataatc aatagcaagt
    38821 attttatgta ctaaaggagt tttgctctat atagactcaa tttagtaaaa ttatttaata
    38881 aatattacag actataggta aataatattt acaaataaaa ttaagtttat tcccttacac
    38941 tataatattt cttaattata ttaattgcaa tttcctttac ctttggccct gtaatttctt
    39001 tattggaatc atctgattta atagcaaaat aataggtgtc accttcacgc tctagcatac
    39061 caacaaacca tgcatccaca cgtttatttt tttcatcaaa tcctgttcca gtttttccat
    39121 atacatttat gtttttatca tttacatcta ttttcataat atctcttaaa atattaatat
    39181 gctctttttt aaagtttgta tctccctcaa atatttttgc cattgtttgc acttgttctt
    39241 ttggagatat ttgtaatgaa gattctaacc aaaatccatt taaatgtccc tttccatttt
    39301 ttaaatctcc ttcccattcg ctaatatcgc aattcccata ctttaaatca tctaaaatgt
    39361 tttggacaga tttagcatca actttatcta tcaatttttt gtagtaccaa acacaagact
    39421 ccttaaatgc ctcttctaaa ctaagatttt tattccaatt tttatttgga tactctgtac
    39481 catcatatcc cataactgat tcttttgagt ttattacccc tttttctaaa ccaattaaag
    39541 ttgaaactat tttaaaagta gaacatggtg accttcttgt ttcaattaat tctttgttat
    39601 atatattata ttctttattt ttagtattat aaaaaatagc tcctccacta ataccttcaa
    39661 aacaatcact atagtcaacc atattaattt tttctttttt gtttttatta ttgttttcaa
    39721 ttttggattt atttaattca gacttattta attcagattt tatataattc ttatcagttt
    39781 tttgatttat atcgttatgt tttttcccta cataatacat taccataact acaccaacca
    39841 ataagatagc tatccaaata aaattctttt ttcttttcat aatttatatc atctcccata
    39901 tttaaattta taacctacta ttaaaatagt agacattatt tgtaatatac tccaatctag
    39961 gttctactta taagaaacaa gactaaatga atagctgtat ctcttatatt ttcttcacct
    40021 agtacaatta tgttgattaa acctattgcg aagataatta agtaactatt aataacatcc
    40081 tgtcctcctt ttaatttgtg atataataat tgattccaca tttaaaactt actattgtaa
    40141 tatttaaata ttacaatagt aagttttaaa tgtcaagtta gtttgggaaa gttattttaa
    40201 ttgtataatt gttattacat ttataaaata tggcatgttt aaaattattt tgctattggg
    40261 aggattaaga taagcagtat tataaaagct ataaattata aaatttaata tataatttat
    40321 agcttttact tttacaaata ttgtttttat tgatttatta atgaatttta ttgacaggtt
    40381 atatttatat aagtaaacac ttacataata agtatataat ctacatatct ataagttgag
    40441 tcaattcacc agtatatgtc aacttaagtt tatgcatagc tcgtgtacaa gcaatatata
    40501 acaaactgcg gtcataatca ctataatatg ttttattatt tactgaaggt acaataactt
    40561 catcaaactc taaacctttt gacattttaa tagaagttat ggatactcct tttgtaaagc
    40621 tagaactttc agaagatatt aagtgaacac tatatttttc tcttaacgca tcatatacag
    40681 ctttagcgtc attatctgtc tttaatataa taccaagagt cgcattatca ctctttttaa
    40741 attcttcaat ttctattttt atcttattta tttcatcttg ctcgttatta cattttaata
    40801 aagctggttc ttcaccatgt cgttcaatag gctctaatga agatatgtct tgaatctttt
    40861 tggcgaaatt aataatctca aaagtagaac ggtaactctt attcaacata actagttccc
    40921 catcattata tagctgtttc atatcatcta gtgtatgagt gtgattagga ttaaccaatt
    40981 gtccaaaatc tccaagaata gtctttttac acttaaaaag taagtttatt acagcatatt
    41041 gaataggtgt gtaatcctgc atttcatcaa taacaacatg tcttataatc ttatcttctt
    41101 gtataccttc atatgcagca tgaatatata taaatgggta tacatctgac cattcaagag
    41161 ttttttttgt tgccatcaca aacatgtttg atatattcat ttgtttaaag aaatccttat
    41221 atagtgtaag agtatttttt attgtcaaca ttccattcaa acttttaagt atacttttta
    41281 cctttggtaa atcttcttcc ataatattat ctgattcaaa tttataatga atgtcttcag
    41341 ctaccttctc taatctcttc ttaactggat atctgttata tgcctcaaat ctactctgta
    41401 tccagtcact tttagctgta aaacttccaa aagtgtagtc ttttggaata aaaatcttat
    41461 ttggcatttg ttttatataa tcatcaataa gttttacaaa atcaagtgta gatttaaaac
    41521 gcactctttc agaccattta gcatcattta tttctaatgg gtctttctca ctttcaaaat
    41581 taataactct atctaattgt acctctgcta tattttcaaa acttaattca cataatggct
    41641 cttctccaag ctcaggaagg acattggaaa tatagtctcc aaagacttta ttaggtgata
    41701 ggataattac attatttgct gatattttat ctttaaatcg ataaagaaga aaagcaattc
    41761 tgtgtaatgc tatagatgtt ttcccagaac cagctacccc ttgaataatc agtgtatcag
    41821 ctttatcatt tctaataatt tgattttgtt ccttttgtat tgttgaaatg attgacttca
    41881 ttttttcatc tgaagtgtga ctaagttctt tttgaagaat atcatcttgt atattaatgg
    41941 aactttctag tgcatattct aattttccat ttttaatttt aaattgtcgt tttcttgtta
    42001 tttctccatc cacccatcct attggagcat catatccagc ttttccgatt tcataatcat
    42061 aaaacatact ggcaattgga gaacgccagt caagtattat aagctcatct tcataatcaa
    42121 atgcaaatct accaatataa tacttagatt catcatccat atctttcagc ctaaaatcta
    42181 ttctagcaaa gtatggagaa tctttgattt tattaatctt atctcgaact tttaccataa
    42241 aagccccata gttatctatt tgttttagtc cttgttcatt ttgaaacatt tcatgtgggt
    42301 ctatttcacc acgatttttt accatataca ttttagcgtc catataatcc ttatcaagct
    42361 tgtcaacaag attttcagct ttcttaaaag cattgtctag cttgttattt atctctatta
    42421 gatgtgcaat ctcatctgga aaaaaagtta ataactctgc atcatattca cacatattat
    42481 gtttagaatt agatgcaata atatcgcaat ttactacttg tttattttca gacatttaaa
    42541 tcctcctttg ttaattaagt acttacactg aatcattata cttaattgtg ttttggagtt
    42601 tttaatcatt cttgctattt aacttaaatg ttagcttttt agctttaaag ttcctttttt
    42661 aaaaaactaa atctttctat atttattacc ataagtacac ttacaagcaa aatacattaa
    42721 aagcagaaat gctcttatat ataaaataat tcaaacaaaa aatatgtggt aaacttttca
    42781 tttaatctac aattaatttt gtctattatc tctttcttct acacaaggaa gaataaattt
    42841 ttgagtagga tatccaaatt ccacaagcaa ttctgcttct gcaaagccca acttctttat
    42901 catatctcta tgtcctgtat cagctttgtc accctctcga aaggtagtaa ttgaaatatc
    42961 tgtatctatc aagaaatctt caataacttt cttcaaaaac acttgtgcaa tgtctgaatt
    43021 acgatactgt ggatgtattc caaaaaaatc aatacttcta gtttctctat taaacaccat
    43081 atttgcaata gcgacattat taagttttaa tattaaagct ctttcttcta aaatatactg
    43141 ttctaattga tatatatatt cttcctcttg caaatttgga aatccatcta tgactaatct
    43201 cactaaattc atccataatg gtatgtcaga catacttgca agtttaattt ctttttccaa
    43261 ctctagtaca ttctttgaaa ataggttatc ttttttcaaa acaaatctta gctgcaaagg
    43321 ataaaattct tgttctttcc tatatttatt tggagatttt ttatacatag ctttaaatat
    43381 agaagtgaat gcctgttgac tttcatatcc agctatcaat gcaatttcaa taattggttt
    43441 ctttgaaaat accaataatt ttgctgcttc tgtcaacttt ctccgcttta tgtaatcatg
    43501 cattgttaga ccaacagaag ttgtgaaagt tctatgcagg tgatattttg agtaatgaac
    43561 agcattagca actatatcta aatccagctt ttctgataaa tgctcctcta tatagtcaat
    43621 agcagttatg acattaatta ctttgtaaga cattgcagct tccctccttt tatccatgca
    43681 acattatatc aattattact tagttatatt taatgaatct tgctatttta taaacaaatt
    43741 tgttgtttaa gttttcaatt cataaattta atatattact ttttactttc tgattaaaga
    43801 ctctaatcct ttaattttca ataacttttc atttagtaaa tctaaaaagt tatttattat
    43861 tttcttctta ttagattcct tttatattcc ttaattaaat gataaatatt tatattatac
    43921 gactaaaaac agctttttac aattatttaa tatgattaca aacttaccct atcttaaagt
    43981 ttaatggggt cctgttatga atctttttat tttaatttaa gctcttcatc agtttatttt
    44041 atatattgat gtatttgatt tcatacaaaa aagagtgctt catgaactaa aaagttcatt
    44101 tttaaacact ctcttttatg taaataaatt tctattctat tttgaaatat tataatttga
    44161 cttataatct gttaatacca aaaataataa attgtacgtc taagaataga ttctaattag
    44221 ttgctcagta gtaaaatctg tttttaacta atcctgcttt gtatgttgaa catattctat
    44281 aattgtacca tctggatgtc tcaatgtcat attatatcct gtaggaactt ttttcttatc
    44341 tcttactaca actgctccat tatccaatag ataacttctc cattcatcaa cagaatcaac
    44401 cataaaagta gattttgttt ctataaatgg tttaagagca tcatcagaac cagataatag
    44461 taaaacattt cctatttgcg ctaattccaa cccaacttct ttataggaaa aacgcaaaga
    44521 acatttttct tttaaaagat tttcataaaa aggtatggct ttttctatat cttgaatata
    44581 aaaacgtgtt aatatatcat atattttcat attttccccc tatatatcta tataatatta
    44641 cctctaaatt ataccactct acaaattata taacaataaa ttaagcatag ccttttgagc
    44701 atcttaagta gaccttaaca atatattggg gttacattag aatacataaa ttaaatcacc
    44761 ttgatattat ttaggataaa atttatgtag tagcagtaaa tgttcttcta attatcaact
    44821 aacaaacgat aatctttaac tttctatata atgggtaaca tcattttcgt aagatattca
    44881 ctagctggac gattgatatc atttaaatac tggtaatata tatcgccatg aagtttatat
    44941 ccattgctat ttgcccatga aaataagtct tctaaagtag ggtcttgtaa ttcataaggt
    45001 cctaaatcaa ttgcagttat tattttctgt gcaggaacaa tcctttgtag aatctcattt
    45061 ttcccatcta tataagtggc tacaggaaac cctacttcaa catccaattt tgctaaatcc
    45121 atgttatgaa aacatacaat cggtgctcct gaagataaaa ttcctctctc ctccagatat
    45181 ttagaaattt tttcaaaact ttgttttgaa aactctgaaa actctaccat aaagtcaatt
    45241 gtttttcgta ttgccaaaaa acagtactca gattgttcag ctattgttat attggatatt
    45301 ctagtcattt tattcctcca ttactttatt ttcatttcta atctttttat accaaattat
    45361 acgaaaattt actgtgtaac taattatttc atatgaatat aaaacataac ctccttccat
    45421 ttcttatatc gaaagtatag caaaaataat ctcatcattc ttgtcacaca gtgctcttat
    45481 aatcagaaca tacttaaaag acttgcctaa tttttaggca agtctaaaag aaatcttttt
    45541 ctataaaaaa tttatacaaa caattaaaag atcaaaatat agactaatat aaaactataa
    45601 atttactaat ctgaacctcc catggctaaa gtcacaagat tactgattta tagaatttcg
    45661 cacactaaaa tatgtcttac taattcccca caggctaccc ccctagttcc tacgttctta
    45721 cataagttat ctattatcaa acatcaaaat ttaaacaagt ttcaggttca tcttctattc
    45781 ttgctatttt aaacttacat gcaattaata ctaaagatac tattggattt ataatattta
    45841 ataaagcgta tggaccatat gttagtgcag atacacctaa tgttgctgtt atatacgccc
    45901 cgcatgtatt ccatggtact aatggagagg ttaaagttcc actatcttct aaagctctcg
    45961 acaacatctt aggatgtata cctctctttt tatattcctt attatacatt cttcctggta
    46021 taactataga tagatattgt tcacctgcta ttatattggt aattgtacaa gttaccatag
    46081 tacataatac tgttccaaat acaccctttg ctaatcttag taaagaagat gctactactt
    46141 caagcatacc tgttttttct aaaatacctc caagagataa agcgcatatt gttaaggata
    46201 cagtggacat catattcata atcccacctt tagataataa agcatctacc tcctgtattt
    46261 gtgttgacga aacatatcca ttttgtgctg catctaatat cgtattcata gattctcctt
    46321 gaaaaactac tgcaaacaat actcccaata cagcacctac tataagccct ggtatagcag
    46381 gaactttaaa tacaaccaat ccaattacta ttgctggagc taataataat actggtgata
    46441 aagtattaaa tgtagaatct aatgtagacc taattaattc tatttgtttt atatctagtg
    46501 cttgacctga atacttcatt ccaattacac cataaattac taaacaaatt aataatgatg
    46561 gtatagtcga atatagcata tattttatat gtgtaaacaa atctgttcct gccattgctg
    46621 gagctaagtt agtcgtttca gataaaggtg atagtttatc tccaaagtaa gctccagata
    46681 ttatagaacc tacgattaat ccaattggca tacctaaact ttgaccaatg cctaaaagag
    46741 ctactcctac tgtacccatt gtgctccaag atgaacctgt cgcaagagat actattgcac
    46801 agactaatgt tgatgctact aaaaaaatat ttggtgataa aatttttagt ccccagtaaa
    46861 tcatacatgg aactactcca cttactatcc aagttcctat aagtacgcct attataatta
    46921 tgattaagat tgcttgcatt gccatcttta tactattaaa catacttaat tctaactctt
    46981 tccatttaaa tccaagtcta tatacagcca caagtcctgc tactgctgct gctcctatta
    47041 gtggtacatg aggtgatgaa ccatacacta atactcctag acttaaaaat aatatcaaac
    47101 aagtaattgg tatcattgca tcaaacagac ttgcttttct acattctcgc tctttcataa
    47161 aatagtttcc tcctaattat ttaaacacta cactataatt ttttttgaaa catatttgct
    47221 cgtactagat gacatgatac ccccataagt taataaaaat tctaaattgt cacctaattt
    47281 ataatcatat tttgtgttac ttacatctag tatcatgtgg tctgaacttg cccctaatat
    47341 ttttatatca gtgtctattg gaattaaaga gtcaatattt atatcttgtt tacctatagc
    47401 tatgattgcc ctttttaaga ttcccttatc ttcataaaca ggtttatttc taaaggcatc
    47461 aactcctatt tctccaatag gaactgatgg tttttcttta cattcaacta cttggcatat
    47521 taatttaaat gcatcttgat atgttccatt tatattttca ccgtatgccg tttctctacc
    47581 aagtagaatg gactcgccta ttctcaaatt agtaatacct tctggcatat tattatttat
    47641 caacagatga atagaacttg aattacctcc agatactatt tctagattta ttccaaattt
    47701 ttcttccatt ctttttgcta tacttaccag tctgcctata ttttccctag atggtataat
    47761 agctccataa caggttaagt ttgtagctat accaattatt tcaatatttt ttaatcttac
    47821 tatctctctt acattactaa aaaagtcttc ttcataaaag tatccttctc gtaaatcgcc
    47881 taagtcaaac ataagaacta tcttatgaat aatattttta ctttcacata attcatttaa
    47941 tttctttatt gttcctaatt ctgagttaaa actaatatcg caataattga taacttcttc
    48001 taattcactt aacattggga ttctaatcaa tatcttaggt agatttatat cttgtaaatt
    48061 ttttaagttt tgtattcttg aatctgctat atggtctatt ccttcactac atatttcttc
    48121 tactatttct ttttgtgcac aaaatgattt agtaacaaaa gatatcttaa tatttctttc
    48181 atgacacatc tgcgaaatta attttgcatt atgctttaac ttttctatat ctatttctaa
    48241 cctaggatac ataatctttc ctcctcaaaa ttcaaacttt ttaacaggag cttaagagat
    48301 gcttcttcat attttttaga taaaacaccc atagatgcca taatatagtc attatctatt
    48361 aatatctttg ttttttcctt tggaaatagt aattcattat ttttattgta tttgagcata
    48421 ttatctaata tttcaattct actagggagc ctagtaagtg ctccccctgt accaactata
    48481 tatcttactt ctgttaaatc tttcccttct gcaattttga cttttccagt tgttccatag
    48541 atatttctta tttttcctga atgtcttagc atagctttaa caacagcttc ttttgtcaac
    48601 ctctcaacaa ataatatttc ttcttttgat tttggtattg gaggataatt ctctattact
    48661 gcatcaatat ctatagatag ttcactttgt aagttttctt tacctataac ttctactata
    48721 ttcttcatat ttacatatac acctaaatcc ccctcaacac ttctctttgc agtaggttca
    48781 ggattaacca aaattttatt aatatagtca cttccatctg taacagaatg tacatcagta
    48841 gttgctccac ctacatccag agttaataaa tctccaatat ttttatataa aagtttagaa
    48901 gcttccatta cagctcctgg ggttggagta atattttcat taaccatttc tttaatgtat
    48961 ttcattcctg gtgctgtagt tatgtgttct tcaaatacac tttgaatgat tcttcttgta
    49021 ggttctatat tcaataaatc tattttaggg tatacatttt cagttatata aagcttataa
    49081 ttagtatctt caaatattaa ccttatttcc tcttgatttt caacattacc agcatatata
    49141 actggtatgc ctagattcat agaagctatc atttctgcat tataaatagc tgtatctctt
    49201 tctccataat cgactccacc agctattaaa attatgtttg ggtttatttc ttttatttta
    49261 tttaaatcag ttcttttaat tctacctgat gttatttgac gaataacagc tccagcccct
    49321 aaagctgctt ctttagctgc ttttactgtc atatcataga ctaatccatg aacagtcatt
    49381 tttaatccac cagctgcact agaagttgca aacatatcat tatactctat atcatctaca
    49441 gataatttat tagctaaatc tttaatagca cctgataagc catttcttac atcgccacct
    49501 tcaaacactg ttgttggagc ttgaccttga cccaaaaata taggattgtt agtattaata
    49561 tcgtggaaag catttaccac tgtagtagta cttccaattt cagctaccaa cacatctatt
    49621 tttaaagaaa ccttgctatt ttccattatt tattactcat ttctcttctt ttttcaacta
    49681 agaaagtggc tacatgtatg cctttactgt ttcttccaaa tccagcatct atgccttgtt
    49741 caacagctat ttcaggtgtt acttgtgttc ctccacacgc aatcattact ttatctctta
    49801 tacctttttc tacacaataa tcgtgtaatt ttttcatgtt tttatagtga atatcatcat
    49861 gacttattat tgtagatgca agtattgcat ctgcattcag ttcaatcgct gcatctacca
    49921 atttttctac aggtacagaa gtccctagat agtggcattc tatgccatat ttttcaatac
    49981 ctccatgttt tatatctatt atttctctta aaccaactga atgttcatct tctccaaccg
    50041 tagctgaaac tattttcata ggtttttctt ctatatctgc tcttatttct tcatcagtta
    50101 gtaccttagg ttcttctggt attactaaat tgtttaaatc tatatcaaac ggaactcttc
    50161 ctttcacttc tattctagtc ccttcagatt catgcataac ttctctactt atgacctcaa
    50221 tttcttgtaa gttcattttt ttgacaaatt ccaaagctgc aaactctgct gttcttttat
    50281 cagtaggaag catcattgtt atcattacaa ttccatctgc acaccattcc atttcaggct
    50341 taattttagt tccatttctg tactcatatg attcttttag tctattttct acattatcaa
    50401 aatcgtcaag ctcatcaata tatattattt tgtctggatt ttcaaatgta catccatcta
    50461 ttaacattga tggattatct atagcatttt catcatattg agctacatta ttgtatccaa
    50521 agtgagcagt tacaggtgct aaataatctt cttctctttc ataaacagta ccttcaccaa
    50581 ctccaccttt tatttttcta gatattccat caccatttct ttctggataa tttccagaat
    50641 caacaaaaaa tccttgttcc acagattcaa aatatccacc aacttcagat atttcttcca
    50701 tatataatac agctctttct ttaagttctc ttgctttgtc tcttaattca cccacatctt
    50761 taagttctat catatccata agcccatcca taccaactaa agcttgcttt gctgtatcac
    50821 atgcttctat attgtatata tgccaaggta catttctacc ttcatcaggt gttatagttg
    50881 attgtatgtc agctctagtt aatactgatg ttaataagtt taatacatga gtaactgttg
    50941 cttctctagt tgatgattcc atatatttag tattcatttg agctctcatc ttataatcac
    51001 taaataactc tctaagtgca acagcgtaag gtaagtctat ttttaaacat ggtgctggag
    51061 gtgctgttgg tggaactgtt gataagcata tattactttt atctatacct attttttctg
    51121 aaaatataga gtttaaagcg tgttgaacca taagttcagg cattaccttc catgcttctc
    51181 tagcagtagc attagcatta tgtgctccat caatttgagc tatattggca aaagccatta
    51241 ttttctttgc ctcacaagca tctacaaaag accttaccat gtttatattc ctatacagaa
    51301 cattatattg tgggtcttga tgtgctccat tgactccttc ctcagcaaac attactgcta
    51361 cttcaggacc agcaacacca cttacatatg aatggtaatt aataggtcta ccaacttctt
    51421 cttctataaa atcaagagcc ttcctttgag ctcttacttg ttttcttgtt attggaactc
    51481 cacctatacc ttgtggagtt ccttctataa gcccatcaaa atgtgattgt ccagcagttc
    51541 tgataaccat tagatggtca gctccatgat gagctgccat tctcattctt cttatatcat
    51601 cctcaaatct accagaagct atttcagtag taataactgg acttggttga gggtctatat
    51661 tattgaaata cttagatgat ggtagcccta cactcttctt taaaggtttt gtacagtctt
    51721 tatatttaaa aggtcccatt tcaagatttt ccaagtgttc tctccaaacc caacctcttc
    51781 gttttggttt atatttatcc aaatctttta atatatagtc tatatccaat tttttatttt
    51841 cctttaacat gattctaccc cttctctaaa taaacatgta acttcatccc aatattcacc
    51901 cttaactaat aacaaacctg cttctctaac cgttatattt ttagatttag ccaatttata
    51961 aactacatgc cctgctcctt tacctataag acctctttcc ataacccctt ctaaaatagg
    52021 ttttacttca agagaagaaa atcccattct caacaagaca gacctttcta ttgatggtgt
    52081 tgtattcttt ttaccaagtt ctatcattgg ttctactatc tgggtagcta aactccaaaa
    52141 cctttctttt aactcatcat cactcagttc ttgaagatgt tttcttctaa cttcaaaatc
    52201 atcctctctt tttttcatga acattcctcc attcgtattg atttaatcga attttctgaa
    52261 tttttatttt atattaaatt aaaaaacaat aattactata aattatttaa tacatcaaca
    52321 acaaaatctc ttgaactttt tacttcttcc attaaaaatt ctatatctcg attacttagc
    52381 ttatcaatat tatcaatgtg attattagat atacaatttt ttataaatga ctttcttatt
    52441 ttattcatat caatatcaac acatttcaat aattctggtc tttctggtaa tattaagttt
    52501 actcctgcaa tttcttctct tggatttcca aattttatat ctattccatt ttctcttgca
    52561 aaagtaagct gtggacatat atgtttacct gcgcctgtat attctgtttc ttgtaccact
    52621 ataactttat cctcatctaa ctcttgagct aaagagaatg ctgctgctaa agatgtattt
    52681 cctgctggac ctctttctat accttctagc tgtgccaata attcagttgt ataaaaaact
    52741 tctccttgtt ttactaatac atatctatcc atatatctaa gtggtctagc tgcagaccta
    52801 ggtacatctg acctatcagg ccatgtacag tatggtatcc caaaacctgt atgtcctgtt
    52861 gtaaatgatt ttttattaaa ttgattatca ctagccatgt gtaatccttt taaatctaca
    52921 cttgctccta ctactttagt attaatagaa ccagctttta tcaatcctct tgcagttcca
    52981 gtaagattac caccacctgc atttgtacaa acaactatat cagggtcttt tttatacttt
    53041 tctctaaatt ggatagctaa ctcataacct aaagtttcta cacctgcaat tccaaatggt
    53101 gaatataatg atgcgttaaa atagcctgtt tcttctaata aaactaagaa cgtataaaat
    53161 aactctggtc caacagttaa ctgaacaact tctgctccat atgcctcaca ttttcgtgct
    53221 ttttctataa tttcaggttg acctacacca ttagaatcat aacattcttg aactattata
    53281 cattttaatc cctgcattgc cgcttgtgat gctacagctg caccatagtt accactagta
    53341 gctgctatta cacctttata tcccatttgt ttggcatgat atactgcagt agctgctctt
    53401 cttgctttaa aactaccaga tgcattcatt gcttcatctt ttacaaatat tctagctcct
    53461 ttaccttctt tagcacattt tctagctaat ttagtcaaat ttttaagctc tattaaagga
    53521 gtatttccaa cagcatactg tgattgaatt gcttcaatat cttgtaatgt atatcctgtt
    53581 tcactcatca ttttctcata atcaaaccct attccctctt gttcaaaata agaataatcc
    53641 aatccgattg catttttcat tatttcgtta tttcttccca taacagcttg ataactcata
    53701 tctttcattg atgttgaatt attcattata tacacctcca aataatattt ctttagcttg
    53761 gattcctatt tggagtaact ctggtataca tttaccataa tcatgagtat aatatggatt
    53821 tattttaagt aaattacctt ttacaagtct gcctgtaatt gttttaactt ctactaagtc
    53881 gcctatagaa gcatcacttt gtataaaacc tttaacccac atttctaagg atactttctt
    53941 agtatcctct ggaacttgtg gagctctttc ttctggagtc aatactatat tgtgtataat
    54001 aacccaatca ttaattttag catccatttt ttcatcccct tattaattga ctattgcctt
    54061 actaattaat ttactgttat tttaaattaa acattatttt accaattagc taactgttat
    54121 cttactaatt agctagttgc tatcttacta attagttagc tgttatctta ctaattagcc
    54181 tttgattctt cttccaattc aacttctatc tgatttctta tatctcccat tattgctcta
    54241 ggtactggta tatccaacat agttttaaga cctggtctag catttattat taatggaata
    54301 gagtttacta tcattgcata agtacctact cctccaggta tttcaggatt tatttgtaaa
    54361 tcaatgtttg gtataccttt tattgataca tagtctcctg ttttaatacc ttctgcttct
    54421 ggtattatct gttgtggatg ctccatttca attaaaactt ctccatttac atatccatat
    54481 ccacattgtc tacaaccagc aacatttcca gccaatgcct ctccgtactt agattttcta
    54541 tctactgtag tcattattgg ctcttttgtt tgttctatct tatctaattt ccaacctaat
    54601 ccatcagcta tcatatttat tgattctaca aaacctacat gacctgctat agtcttatct
    54661 tctacacctt ttataaattc ttcccttgtt actccaacac cttgttcctc cataacagac
    54721 tttccaaatg gagataaatc atttactctc ttagctttta tactagtaac ttcttcacaa
    54781 gtgcctgaaa gcgccaatac tagtaaatct aatacaaatc ctggatttat tcctgttcca
    54841 agtatactta ctccattttc ctttgcaact tcatccattt ttttagctaa atctgcatca
    54901 tcagcttgtg gatatgccat ttgttctgct gttgatataa catttatttt tctatttaat
    54961 aagaaaatta ttttatcaaa agccttttta gtaaatgaat cagttgctaa caaaacaaca
    55021 tctacacttt tttctctaaa aacttcttca taatttttat tgataataac ttctggtctt
    55081 tctcctcttt caattcctaa aacattatac atactttttc ctgaagtact tctactacaa
    55141 acagatacta tttctatacc ttctttcttt agaatcatat tagccatacc tattcccata
    55201 gctccaaatc cccatattcc tactcttact tttctcatat atacaccctc ctaaaaattt
    55261 atatattctc aataattagt taaagcataa atcgtgccaa ctttaaaatt aaagtaaata
    55321 tttattgaaa cgttgaaact ataattattt tattatgtat tttcataaat aaaaaataat
    55381 atttcttatg agaaataaaa aaaagagcaa aatattttgc tcttttataa taattttgct
    55441 cctctatttt gcttttttat tttactttga ttttgttttt ttatcttgct tatttataat
    55501 attttgatat tatatttttt tattttatac tgtaaatttt gcctattcat acctaaaatt
    55561 gatgcagctt tagttatatt gtaagatgcc ttttctaatg attcatatat atacttttct
    55621 tctatctcac tgataatctt actaagtgtt ttgtcctcca aatcgtttaa agaataattg
    55681 ttactctctt tattctcttt aagattgtaa tagtcgttta gattaatcat acttgtttct
    55741 atttgtacct catcttccat catactcata gtacaataaa ttatgttttc tagctctctt
    55801 atgttacctt gccaatcgta attttttaaa attttaatag cttcatctga aattcctact
    55861 acttttttat ttaacttttt attatacttt gaaataaatt ttttgcacaa tagaggtatg
    55921 tcctcttttc tatctctaag aggaggaatt tttatatgaa ttatgttaag cctataatat
    55981 aaatcttttc gcattttatg ctcgtttaaa attacctttg gactttcatt tgtcgttgca
    56041 ataattctta catctatagg tatatcctta gttcctccaa cccttcttac ataaccttct
    56101 tgtaaaaccc ttaatagttt agcctgtaac ataataggca tggaatttat ctcatccaat
    56161 aacaatgtcc ctttgttagc ttgttcaaat aagcctggcc tatctattgc tcctgtaaat
    56221 cctcccttgc tagttccaaa aagaatacct tcgaatagtg attccggcaa agctgcacaa
    56281 ttttgtgcta taaatggata gtttttccta ttacttccat agtgaatgga ctggctaaat
    56341 aactctttac ccgttccagt ttcaccgtat ataaatactg aagcatcaga cttgcaagct
    56401 ttcttagatt tagctattgc ttctttaatc aaattacttt cccctaaaat atcatcaaat
    56461 tcataaccct ttttttgtct aatactaact ttattgacta aatttttttt ataatttctg
    56521 tttagtaaat tttctatcgt tgtcatattc tttgatatct caagtgcccc tacaaactcg
    56581 cctttaatat caattggtat tgtagtatta attgctgtta tctctcttcc atttttatcc
    56641 agatattttt gaatattgtc tttgataact tccctttttt ccaaaacttc taatagtgta
    56701 ctcctgttta ttttcatttt gttgactata tcaaaaaatg aattggttaa aatatcgtgt
    56761 ctgttcattt tttccatttt ttgtacagaa ttattgtaga ttatagtttt cccgtcttta
    56821 ttaacaaaat gtaccccttc atcaatattt tccaatattt cattcattat tttaaaaaat
    56881 atctcttggt tcaactatat caccccagta cctaaatata taaaaaccca aaaaattaat
    56941 aaaatgttgc tattgtattt atcttatcat atatttatga tattttttat gtacattata
    57001 ttaatatatt tttaagtata tttaaaattt tatttatata tctttcatta tggttatata
    57061 caatatttga attgt
//
LOCUS       DAYASV010000010        56111 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYASV010000010 DAYASV010000000
VERSION     DAYASV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.010"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..969
                     /locus_tag="ACGI68_000658"
     CDS             <1..969
                     /locus_tag="ACGI68_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFH0137116.1"
                     /translation="LIWINILNPVFIKNEIKRKKIQIIIFFFINFGLTYSLGLLLYYN
                     KFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLIYSLLIVLKLLN
                     LINNNEFIIINLILTFISSLSIIIYSSNKKNLYPVKNIKLGSLLIIYFMISVIVPTSF
                     ILILQGAQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDKFGKKLGVII
                     LGMCWSLWHLPLIFTLYTPEAPILGIILRSIHIVGISIFLGYLYIKTKNIWFCAIIHV
                     LNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
     gene            complement(1068..2030)
                     /locus_tag="ACGI68_000659"
     CDS             complement(1068..2030)
                     /locus_tag="ACGI68_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFH0137117.1"
                     /translation="MNKSWSNLIIKNKDKKVRSGWIIIAVMATFYILQYYLSMILVEI
                     IRKILVATGDINLKTNYFSEYANWLNDVGLQIVFQILTDSLMIIIPIIVWRFIMKNPI
                     SEMGLHSYRIKKKEGYVGMLLGIFNCTVIFIITITIGGGHVTSWVPKISPLTVSWILV
                     FILVAFAEEILNRGFFMAVLRRCKNIYFIMIVPSVIFGLIHIWNPDVTFLSVINIIII
                     GILFSYMFIKSSNIWMCIGYHFAWNVFQGIIYGMPVSGLQVPGIITTQITHGNLLNGG
                     MFGIEGGILTTFVNLLSFIFVWYYYRDSKYDFVSDKVNVTNIKN"
     gene            complement(2346..3296)
                     /gene="hpdA"
                     /locus_tag="ACGI68_000660"
     CDS             complement(2346..3296)
                     /gene="hpdA"
                     /locus_tag="ACGI68_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HFH0137118.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSVENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(3289..3546)
                     /gene="hpdC"
                     /locus_tag="ACGI68_000661"
     CDS             complement(3289..3546)
                     /gene="hpdC"
                     /locus_tag="ACGI68_000661"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HFH0137119.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            complement(3550..6258)
                     /gene="hpdB"
                     /locus_tag="ACGI68_000662"
     CDS             complement(3550..6258)
                     /gene="hpdB"
                     /locus_tag="ACGI68_000662"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HFH0137120.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            complement(6555..7571)
                     /gene="tsaD"
                     /locus_tag="ACGI68_000663"
     CDS             complement(6555..7571)
                     /gene="tsaD"
                     /locus_tag="ACGI68_000663"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFH0137121.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKNGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDNGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            complement(7577..8053)
                     /gene="rimI"
                     /locus_tag="ACGI68_000664"
     CDS             complement(7577..8053)
                     /gene="rimI"
                     /locus_tag="ACGI68_000664"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFH0137122.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            complement(8043..8759)
                     /gene="tsaB"
                     /locus_tag="ACGI68_000665"
     CDS             complement(8043..8759)
                     /gene="tsaB"
                     /locus_tag="ACGI68_000665"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFH0137123.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            complement(8759..9211)
                     /gene="tsaE"
                     /locus_tag="ACGI68_000666"
     CDS             complement(8759..9211)
                     /gene="tsaE"
                     /locus_tag="ACGI68_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFH0137124.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            complement(9350..10390)
                     /locus_tag="ACGI68_000667"
     CDS             complement(9350..10390)
                     /locus_tag="ACGI68_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0137125.1"
                     /translation="MHLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENMERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYLCLEAIKKDCNALKYVRNKTEGLIIKAISHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVKNQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSIQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            complement(10719..11324)
                     /locus_tag="ACGI68_000668"
     CDS             complement(10719..11324)
                     /locus_tag="ACGI68_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0137126.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     regulatory      complement(11449..11566)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(11626..12792)
                     /locus_tag="ACGI68_000669"
     CDS             complement(11626..12792)
                     /locus_tag="ACGI68_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0137127.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKALGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     gene            complement(12940..15081)
                     /locus_tag="ACGI68_000670"
     CDS             complement(12940..15081)
                     /locus_tag="ACGI68_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFH0137128.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKESFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DSEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIGEIDEKIKEIGLRELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            complement(15225..16332)
                     /gene="prfB"
                     /locus_tag="ACGI68_000671"
     CDS             complement(join(15225..16256,16258..16332))
                     /gene="prfB"
                     /locus_tag="ACGI68_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFH0137129.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNQQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            complement(16347..19022)
                     /gene="secA"
                     /locus_tag="ACGI68_000672"
     CDS             complement(16347..19022)
                     /gene="secA"
                     /locus_tag="ACGI68_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFH0137130.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            complement(19154..19705)
                     /gene="raiA"
                     /locus_tag="ACGI68_000673"
     CDS             complement(19154..19705)
                     /gene="raiA"
                     /locus_tag="ACGI68_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFH0137131.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQENYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            complement(20258..20932)
                     /locus_tag="ACGI68_000674"
     CDS             complement(20258..20932)
                     /locus_tag="ACGI68_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFH0137132.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            complement(20935..21987)
                     /locus_tag="ACGI68_000675"
     CDS             complement(20935..21987)
                     /locus_tag="ACGI68_000675"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HFH0137133.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            complement(22036..22995)
                     /locus_tag="ACGI68_000676"
     CDS             complement(22036..22995)
                     /locus_tag="ACGI68_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HFH0137134.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            complement(23088..23609)
                     /locus_tag="ACGI68_000677"
     CDS             complement(23088..23609)
                     /locus_tag="ACGI68_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdB-related putative oxidoreductase"
                     /protein_id="HFH0137135.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEQFLNE
                     SKVIATLFCGDEFFVNNQEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCYQ"
     gene            complement(23620..24954)
                     /gene="celB"
                     /locus_tag="ACGI68_000678"
     CDS             complement(23620..24954)
                     /gene="celB"
                     /locus_tag="ACGI68_000678"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFH0137136.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLVSSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLLILGIFMYIPFIKMLDKQYLREENETVEEEDDDISFDDLVL"
     gene            complement(24993..25319)
                     /locus_tag="ACGI68_000679"
     CDS             complement(24993..25319)
                     /locus_tag="ACGI68_000679"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0137137.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            complement(25343..25651)
                     /locus_tag="ACGI68_000680"
     CDS             complement(25343..25651)
                     /locus_tag="ACGI68_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0137138.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            complement(25792..27705)
                     /locus_tag="ACGI68_000681"
     CDS             complement(25792..27705)
                     /locus_tag="ACGI68_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0137139.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMLTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSNNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIKIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            complement(28037..28819)
                     /locus_tag="ACGI68_000682"
     CDS             complement(28037..28819)
                     /locus_tag="ACGI68_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFH0137140.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKMVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(28822..31041)
                     /locus_tag="ACGI68_000683"
     CDS             complement(28822..31041)
                     /locus_tag="ACGI68_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFH0137141.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIASKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEENFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(31305..32402)
                     /locus_tag="ACGI68_000684"
     CDS             complement(31305..32402)
                     /locus_tag="ACGI68_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137142.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            complement(32717..33910)
                     /gene="metK"
                     /locus_tag="ACGI68_000685"
     CDS             complement(32717..33910)
                     /gene="metK"
                     /locus_tag="ACGI68_000685"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFH0137143.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEETKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(34047..34150)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            34339..34848
                     /gene="yyaC"
                     /locus_tag="ACGI68_000686"
     CDS             34339..34848
                     /gene="yyaC"
                     /locus_tag="ACGI68_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HFH0137144.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(34959..35387)
                     /gene="fabZ"
                     /locus_tag="ACGI68_000687"
     CDS             complement(34959..35387)
                     /gene="fabZ"
                     /locus_tag="ACGI68_000687"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFH0137145.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNITVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(35403..36422)
                     /locus_tag="ACGI68_000688"
     CDS             complement(35403..36422)
                     /locus_tag="ACGI68_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFH0137146.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(36619..36879)
                     /gene="spoIIID"
                     /locus_tag="ACGI68_000689"
     CDS             complement(36619..36879)
                     /gene="spoIIID"
                     /locus_tag="ACGI68_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HFH0137147.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(36977..37645)
                     /locus_tag="ACGI68_000690"
     CDS             complement(36977..37645)
                     /locus_tag="ACGI68_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFH0137148.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFDDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(37711..38775)
                     /gene="spoIID"
                     /locus_tag="ACGI68_000691"
     CDS             complement(37711..38775)
                     /gene="spoIID"
                     /locus_tag="ACGI68_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HFH0137149.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(38862..40115)
                     /gene="murA"
                     /locus_tag="ACGI68_000692"
     CDS             complement(38862..40115)
                     /gene="murA"
                     /locus_tag="ACGI68_000692"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFH0137150.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(40136..40873)
                     /locus_tag="ACGI68_000693"
     CDS             complement(40136..40873)
                     /locus_tag="ACGI68_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HFH0137151.1"
                     /translation="MKKVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGKTLIYIATPIIKLDY"
     gene            complement(41336..42631)
                     /locus_tag="ACGI68_000694"
     CDS             complement(41336..42631)
                     /locus_tag="ACGI68_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFH0137152.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADDENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWREESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFISNPK
                     HSCTNFGADIPVNSQLIVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(43082..44914)
                     /gene="glmS"
                     /locus_tag="ACGI68_000695"
     CDS             complement(43082..44914)
                     /gene="glmS"
                     /locus_tag="ACGI68_000695"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFH0137153.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            complement(45181..46527)
                     /gene="glmM"
                     /locus_tag="ACGI68_000696"
     CDS             complement(45181..46527)
                     /gene="glmM"
                     /locus_tag="ACGI68_000696"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFH0137154.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            complement(46697..47254)
                     /locus_tag="ACGI68_000697"
     CDS             complement(46697..47254)
                     /locus_tag="ACGI68_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0137155.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            complement(47255..48007)
                     /locus_tag="ACGI68_000698"
     CDS             complement(47255..48007)
                     /locus_tag="ACGI68_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0137156.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            complement(48007..49086)
                     /locus_tag="ACGI68_000699"
     CDS             complement(48007..49086)
                     /locus_tag="ACGI68_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0137157.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            complement(49115..49330)
                     /locus_tag="ACGI68_000700"
     CDS             complement(49115..49330)
                     /locus_tag="ACGI68_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0137158.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            complement(49373..50050)
                     /locus_tag="ACGI68_000701"
     CDS             complement(49373..50050)
                     /locus_tag="ACGI68_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137159.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            complement(50040..51119)
                     /gene="buk"
                     /locus_tag="ACGI68_000702"
     CDS             complement(50040..51119)
                     /gene="buk"
                     /locus_tag="ACGI68_000702"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0137160.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLA
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            complement(51141..52043)
                     /gene="ptb"
                     /locus_tag="ACGI68_000703"
     CDS             complement(51141..52043)
                     /gene="ptb"
                     /locus_tag="ACGI68_000703"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050182 - phosphate butyryltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HFH0137161.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIENTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            complement(52347..53528)
                     /locus_tag="ACGI68_000704"
     CDS             complement(52347..53528)
                     /locus_tag="ACGI68_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFH0137162.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESMEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            complement(53525..54364)
                     /gene="cdaA"
                     /locus_tag="ACGI68_000705"
     CDS             complement(53525..54364)
                     /gene="cdaA"
                     /locus_tag="ACGI68_000705"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFH0137163.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            complement(55348..55421)
                     /locus_tag="ACGI68_000706"
     tRNA            complement(55348..55421)
                     /locus_tag="ACGI68_000706"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55385..55387),aa:Arg,seq:tct)
     gene            complement(55431..55505)
                     /locus_tag="ACGI68_000707"
     tRNA            complement(55431..55505)
                     /locus_tag="ACGI68_000707"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55471..55473),aa:Gly,seq:gcc)
     gene            complement(55516..55592)
                     /locus_tag="ACGI68_000708"
     tRNA            complement(55516..55592)
                     /locus_tag="ACGI68_000708"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55556..55558),aa:Asp,seq:gtc)
     gene            complement(55598..55673)
                     /locus_tag="ACGI68_000709"
     tRNA            complement(55598..55673)
                     /locus_tag="ACGI68_000709"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55638..55640),aa:Val,seq:tac)
     gene            complement(55679..55752)
                     /locus_tag="ACGI68_000710"
     tRNA            complement(55679..55752)
                     /locus_tag="ACGI68_000710"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55718..55720),aa:Gly,seq:tcc)
     gene            complement(55767..55841)
                     /locus_tag="ACGI68_000711"
     tRNA            complement(55767..55841)
                     /locus_tag="ACGI68_000711"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55806..55808),aa:Glu,seq:ttc)
     gene            complement(55849..55924)
                     /locus_tag="ACGI68_000712"
     tRNA            complement(55849..55924)
                     /locus_tag="ACGI68_000712"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55889..55891),aa:Met,seq:cat)
     gene            complement(55940..56025)
                     /locus_tag="ACGI68_000713"
     tRNA            complement(55940..56025)
                     /locus_tag="ACGI68_000713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55989..55991),aa:Leu,seq:taa)
     gene            complement(56031..56105)
                     /locus_tag="ACGI68_000714"
     tRNA            complement(56031..56105)
                     /locus_tag="ACGI68_000714"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56071..56073),aa:Asn,seq:gtt)
BASE COUNT        16932 a        10626 c         6048 g        22505 t
ORIGIN      
        1 ttaatatgga taaatattct aaacccagta tttattaaaa atgaaataaa aagaaaaaaa
       61 atacaaatta ttatcttttt ctttataaac tttggtttaa cttattcatt aggtttactg
      121 ttatattata ataaatttat tgatccagaa aactttgcat cattcatgat gatattacct
      181 ctatcttcag ttgccattgc aaagttttat actgaaggat taaataatga tatgtatgaa
      241 ttttattcac taatcatatt attttttctt atttattcat tattaattgt tcttaagtta
      301 ttaaatctaa taaataataa tgaatttata attataaacc taatattaac ttttataagt
      361 agcttatcaa ttataatata ttcatcaaat aaaaaaaatt tatatcctgt aaaaaatatt
      421 aaactaggtt ctttattaat tatttacttt atgatttctg taattgttcc aacatcattt
      481 atactaatac tacaaggggc tcaacctaat tatagtggta tcataaaatt tatatttact
      541 ccaataacat cattaactac tatttatatt ttttttagtg aagaatatgc ctggagaggt
      601 tttttacaga atattttttt tgataaattt ggtaaaaagt taggagttat aatattaggt
      661 atgtgttgga gcttgtggca cttaccacta atatttacat tatatacacc tgaagcgcca
      721 atattaggaa taatattacg ttcaatacat atagttggaa tttcaatatt tttaggatat
      781 ctttatataa aaactaagaa tatctggttt tgtgctataa ttcatgtttt aaataatagt
      841 gcttttctta tcacaaattc taaagaatca actatcactt accatgatat actaatagta
      901 tctatggttg ttttaatttt ttatgttcca tttctattta caaaagaata taaaaataac
      961 ttggagtaaa tagaatatcc aagttatttt tatatttttg caaatttcca acaatcctac
     1021 agaatacttt aagcttagaa gatattatta aaatttatta taatcaacta attcttgata
     1081 ttggtcacat ttactttatc actaacaaaa tcatacttag aatctctata gtaataccat
     1141 acaaatataa agctaagtag attaacaaaa gtagtcaaaa ttccaccttc aataccaaac
     1201 attcctccat ttaatagatt accatgagtg atttgtgtag ttataatccc tggaacttga
     1261 agtccactta caggcatacc atatataatc ccctgaaata cattccatgc aaaatgataa
     1321 cctatacaca tccatatatt cgacgatttt ataaacatat atgaaaaaag aatcccaata
     1381 attataatat taattacaga caaaaaagta acatcaggat tccatatatg aatcaaccca
     1441 aaaataacag atggaacaat cattataaaa taaatatttt tacatcttcg taaaactgcc
     1501 ataaaaaaac cacgatttaa aatttcttct gcaaaagcaa ctaaaataaa gaccagtatc
     1561 caagaaactg ttaaaggtga aatttttggt acccatgaag taacatgacc accaccaatt
     1621 gttattgtta taataaagat tactgtacaa ttaaatattc ctaataacat tcctacatat
     1681 ccttcttttt tcttaattct ataagaatgt aatcccattt cactaatagg atttttcata
     1741 ataaatctcc atacaattat aggaataata atcataagtg aatctgttag aatttgaaat
     1801 acaatctgta acccaacatc atttaaccaa ttagcatatt ctgaaaaata gtttgttttt
     1861 aaattaatgt cacctgtagc aaccaggatt tttcttatta tttccacaag tatcatgctt
     1921 aaataatact gaagtatgta aaaggtagcc ataacagcta taataatcca ccctgaacgt
     1981 acttttttgt ctttattctt aataatcaag ttgctccatg atttgttcat atagatgtct
     2041 tcctcctaac tattattttt agttgtttct aaaatatatt ctattaaatt ttttcatagt
     2101 agtattttac cacaaaactt ctgcttcaat gaagtaagcg taatatattc tctaactgta
     2161 atttaaacta taaaaaatat ataaagtata tttaaagcaa tatttatgga cataaagcaa
     2221 tataaacaat atataaaaca tataaaactt tacttaatta tttctacata aaaaccacat
     2281 aaaaaaggat gtaactctag cttttagtgt aacatccttt ttttaattaa aacttgtatt
     2341 taattttaga aagctgtctc atgaccaaca taacaagcta taccattatc caaaaatata
     2401 tcttgtaatt cttcaagatg accttcatca acatcaccct tatcagaata ctcatattcc
     2461 ttgcctaatt gaatccattt tgattctcct aatctgtgaa atggtaatag attaatttct
     2521 accaaattat ttttgtgcat aaaagatata atatcactta tattttcaac actatcatta
     2581 aacccagaga taactggtac tctaagtact aatcttccat tccaatcaga atttgcaagg
     2641 tttgatatat tttctaatat taaatcatta tacacacctg tttgttcttt gtgtttttct
     2701 ctatccatat gctttatatc aataaatgca aaatcaatat cgttaaatat tttattaaaa
     2761 acttcatttg atacacatgc actcgtttca atggcagtgt gtatatttac ctcatgacac
     2821 tttaataata cttcatgtaa aaattcatgc tgaagtagtg gctcacctcc actaaatgtt
     2881 actcctccat tacttctcca attgttagaa tcacgtttaa ttacctgcac aagttcatct
     2941 acagtgtaag gttttgcaca taatttaaac gcattataat aacatgagtt gacgcattca
     3001 aaactttcac agtccttaca gatgttccaa tctataactg gcttattatc aagattaaag
     3061 ctcaatgcac catttttaca cttaccatga catacagtac atccattttc atattgacaa
     3121 gataattcac taaacatcat atgtggtctt acagtccaac tttctggatt tgcacaccat
     3181 ttacaactca gtggacatcc atttaaaaat acagttgttc tacaacctgg accatcatga
     3241 actgaaaagc tttgtacatc aaaaatcatg ccttctaatt gcttttgact actcattaaa
     3301 cacatgtcct tcacaagtaa ttgcattcaa tgttgaatat acccagtctt ctttctctag
     3361 gccaacacat ttgcctatac cttcatcatt agcttcaaca aaatttttac aatttttaca
     3421 ttttggcatt acttttatct ctggacatgc tgcatcatct aaattaatca tttgttttga
     3481 taatctacaa attcctttgg ttgcatctac tgcacaaaaa ttcatacaat cactatgctt
     3541 tctcatattt tacacccctt catactctgt tctagcaatt acttcatctt gtattggttt
     3601 acctaattca caccaatatt gagtaaaccc agcaacacgt accattaatt gacgataatt
     3661 gtcaggattt ttttgtgcat cttttaatgt ttctgaatct acaacattat attgaatatg
     3721 gaacccacct tttcttaaat aacttcttgt taaatctaat aattttttag ttccttgttg
     3781 tccttttata gtagttggat gaagttttaa attcatctga gaattttgaa ctactgcttg
     3841 gtcccaaata gttgcagatt caaataatgc atatactcca tttttatctg ttcctgcata
     3901 tgcagagact gaaccatctg aatatgttgt tccacttagt ctaccatctg gagtagccaa
     3961 ggtagcagct ccctgtggcc catgtgtaga aacagatatt tggcaagggt acatcttctt
     4021 agcatataaa gattgtgctt cttcacatac ttttgataac catacctcat agtttttaag
     4081 aatattatct gcatacaaat cattatttcc atatttaggt gcatcaagac aggccttata
     4141 aatagcatca tatttaccag tcttatctac ttttacttgg tctgccatag aataatttcc
     4201 tacttctaat gcatctttaa atccaaaatt atttaatatg gcatccttca tttcctctat
     4261 tgtaaatgct ttgtcatcat atacaagttt cttaagtgct gcaaatgaat tgaccatagt
     4321 aacagtacca caagtttcta cgtttaatgt tgcattataa cgatatccca tatttcctat
     4381 atgttgacct ttgtctaaac agtctggctt tagtagtgaa tttattatag aagtatcaaa
     4441 ttttctccat atatctaact caatgttatt tgctctttct aaaacattta ttgcttgttt
     4501 ataataatct ttaaatactt ctatgacttc ttcataagta tctaatggct tattatgagg
     4561 ctcaaatact tgtatattca tacgatagtc tttaccattc ataagaacaa gttctaaaat
     4621 ttttggatta gctataaagt gaactccaga gccagcagat tgacctgctc cacctgcaat
     4681 agagtatgtt tttccgttta aagttaactg tttccaacac cctggagacg tttccaaaca
     4741 tccacctaat gcaaatgctc ttgcttcttc aacagtcata ccttcatcag caaattgttt
     4801 catcataaat gttgttccat ttgagttgtt tacccatgct ggataaccag aaccaagctt
     4861 tgtacattca gcagctttca ttaagaaatc ttctggaagc ttttcatcat atagcatagt
     4921 taaactaggt tgaggagttc tacatctcat acttgcttct aataataact cttctagttc
     4981 attagctcct gtagaaccat cttcttttaa tccacctata gataaagtat taaatgtatt
     5041 gcctgatagt actcctccat ttacgcccgc tgaagcaaaa cagtcaatac aagtgatttt
     5101 tattctataa agctctaata attcgataac ctcttctttt gtaattagtc ctttttctat
     5161 atcttgttca taccaaggat ataaaatttg accaaaacgt cctatagaca taccagatat
     5221 agcatcctca tttactgatg ctatatgaat tgtataagtc aactgaagag cttccctaaa
     5281 tgtacgaggt tgtttatgtg caatatgttc taatatctcc actaaatctg agtattcttt
     5341 tttagcttct aaatctgtct ctatactttc taaatatttt gcatgttttg cataatttaa
     5401 aatccaagtt tgtaatcctt caataacttg aataacagcc tcataaaaat atagacggtc
     5461 cataccaata agcccatctc cagatgcatt tcctgcaact gcctttttac attcttttgc
     5521 catttctata attccatcta atccataatt taaaggataa aaataattta taacttctct
     5581 accttgagga agtgtatacc ctgaatcaaa catgcatata agtgtactca ttaggttttc
     5641 ttttaaatca taatctggca tcattttttc atagtgaaaa cctaaatctt caacagactt
     5701 tccaacccat tcctttgcca tttttacaag tacaggtact tcttcttttc tcattccgaa
     5761 tttaccagct attgaaacga catttccaaa actttcagta acatttcctc caccagaccc
     5821 aaattttgta agttcatcag ctgtgttact ggctaatttc tttgcctctt ctctcatttg
     5881 ttctccttga gcaacaaaga agctttcact tacccatggc attggaaaag aacctctata
     5941 atggcgtgtc ttttgcatta ctattttctc accaggtatt atatttggtg tcatatgtga
     6001 aaaagcagct ttaagagctt tagctcttct aactactggt atatcaccat ctgattttct
     6061 atattctcta ttataccaat atggaaattc catatcagca gtagataatg tattataata
     6121 tacatctaat aagtcttctg ctcttttact tgcttttttt tcattaaatt cacttaaatt
     6181 ttctttattt tgaaaatttg atttaatatt ttttgcttcc aaaatacttc ttattttgtc
     6241 ttctttactt tgactcattt cttcccctcc ttaatctttc tataattagt ataaaacttt
     6301 ttaagaatga aaaaaaaggg tatattaccc actttttaaa ataatctttc tatagaaaaa
     6361 aataaacttg aagcaaatct aagccaaaga caatctgtat atcctaaaat ttgttgcaat
     6421 tcattggaat ataaagatta taaatagttt tacttcaagt tttaatatat actatattta
     6481 tttttattat atttaatttc acttctactt acttttttga aacaatactt ttatatttac
     6541 aaattcatct aactctactg atttatcttt aaatttggaa ctgcatttaa actcatatca
     6601 tgcgttttcc catttataaa attataatat cctgcacacc ctatcatagc tgcattatct
     6661 gtgcataaaa ttagaggagg atacttaaca gtaataccat tgtctttagc aagctctgtt
     6721 attttagctc taagccccga attagaagca accccaccag aaagagttat gatattgtat
     6781 cctttgtctt ttactgcttt tagtgcttta gtagataaaa cctctactac agcctcttgg
     6841 aaagatgctg ctacatcttc aactactatt tcttcatttt tcattctttt tccatttaga
     6901 taatttaata cagaagattt taatccacta aaactaaaat catagctatc ttcttctaaa
     6961 taggctcttg gaaattctat agcatgttta tttccattct tggccagatt atcaataata
     7021 ggacctcctg gatagcctaa attcatagct cttgaaatct tatcaaatgc ttctccagat
     7081 gcatcatctc ttgtttttcc taaaatttca tacttgccat aatcttttac ttcaaccaag
     7141 tgagtatgac ctccagatac tattaatgtt ataaaaggag gttttaaatc cttatgctct
     7201 atatagtttg cacttagatg cccttctata tgatttactc ctacaagtgg aatattcaaa
     7261 gtatatgcaa gtgctttagc ataagataga ccaaccaata aagcacctac tagcccaggt
     7321 ccatatgtaa ctgcgatatg gtctatatca ttaaaaccta tatttgcttt gtctaaagct
     7381 tcttgaacaa ctatatcaat attttccaca tgttttcttg aggccacttc tggaactaca
     7441 ccaccaaatt ttttatgtgt ctcgatttga gtagagatta tgtttgaaag tacttctcta
     7501 ccatttttta aaactgatgc agctgtttcg tcacaactac tctctatagc taatgttatt
     7561 atatcactca taaactctac acctctttta attggttcca cattataatt gcatcttctt
     7621 tattatcact gtagtattct tttcttattc cacccatttt aaatccatat tttctatata
     7681 aattttgtgc tattttattt gatgctctaa cttctaatgt catagatgca atattattat
     7741 ctttacataa atcaactatg ccttttatca acttgtcccc tatttttttc cctctatagt
     7801 cactatgaac agccacatta tttatatgcc cttcatcaag tataagccaa attcccacat
     7861 atccgacaat ttttccattt aacttagcta ctatatacct tgctacttca ttagacaact
     7921 cttttctaaa agactctttt gaccaataat cttcaaaaca atttttttct acttcaaaaa
     7981 cttcatctat atcttctgta gtcatttctt ctatttttat atcatctact attagttttg
     8041 tattatcttc cattatttag cctcttcatt ttttcatcat attgtacttc agcttgagat
     8101 tttctgatat acataggatt tatcgtataa caagtatgta catctatatt tttattatat
     8161 ttatttaatg ctaaagagca taagcttcct gcgttagata cattattagc tggtgaaggt
     8221 atttttatat tttttatatt ctctaacttg tctttatatt tgtatacagc ttctccaact
     8281 attataaatt cttcattagt tgatacaatt tcatctacta aagcatcaaa ctctacaaca
     8341 tcaacaccat ctaattctat catctctcca ttttcaaact tgtatttagc tgaataaact
     8401 tgatttttct gtgcatctaa tatacaacat atctttctat cacagaaatt tatgttattt
     8461 gctagagatt ccaaagaatt tactgctatt attggtatat tatttacatg ggccatagct
     8521 tttactgtag ccatacctat tctaagtcct gtaaacgaac ctggacctat acatacagct
     8581 aataaatcca tatcttttat acttaaatca ctcatactca acatattttc tatcattggc
     8641 ataagctttt gtgaatgagt ttttttgttg tttactgtaa actcacatat taagttatca
     8701 tcttcaacaa ctgctacact tgctgccata gaagacgtat ccatacctag tattttcatt
     8761 atttaatcaa ctcctcaact atctctttat aaaactctcc ttttggagtt aatatcattt
     8821 ctcttccttc atctttatat ctcaattcta tattcaaata ttcttttgga agtatatctt
     8881 ctattagatt agcccattct attatacaga ttccatttga atttacatat tcatcataac
     8941 caatatcata catttcatca ctactaccta ttctataaac atcaaaatga tacaaaggta
     9001 tttttccttc atattcattt attatagtaa aagtaggact tgttatataa tcttctatcc
     9061 ctaatgaatc tgccaaactt tgtgtcattg tagttttacc agctcctaaa tctcctacta
     9121 aacagataac actgccttct tttaaaagtt tacctaattt atatcctatt tctctagttt
     9181 tattttcatt ttctaaatat atttttgcca tcaattcatc tccattttga tataatcgtc
     9241 atgtgaattt atatcactgc ttaattaatt atagtataag cctagattat tataaagtac
     9301 ttgtctttat ctttttttat ctttcactat tgatggctgt atctacacct tatatacaat
     9361 catcaatagc aatttttaca tactcttttg ctttatggtt ttgcttcaat gcttctttac
     9421 atattctctc agattgctct tttacatact taagagactt atagttctct tttactgctt
     9481 caatacacat tttttctgtt tgatttttta tatacataag catttttcca ttttgtctta
     9541 caatctctat acatatatct tctgtatact tatctatgta acttagtaca tctttaatat
     9601 tatatttttt atttaaatat cttatagcat ttatacatat ttcttctgtc ttattgttga
     9661 caaattgtat acttcttcca tcttgtttga cagcttctat acatatttta tcagtttgtt
     9721 tatttacata ttgtaatgcc ataccatttt gtcttacgga ttctatacac attttttccg
     9781 tttgattttt tacatatttt aatgctttat aattttgctt aatggcctct atacataatt
     9841 cttcactctg ttctctaacg tatgcaagac acctgccatc ttttttaatt gcttctaaac
     9901 atatccttct tgtttgagtc tctaaagcct ttagtatact tacaacatct atattggaag
     9961 aatgacttat tgcttttata ataagccctt ctgttttatt tcgtacatac ttaagtgcat
    10021 tacaatcttt ttttatagct tcaagacaca agtattctgt ttgttctttt acatattcaa
    10081 gtgctttata attatacttt attgcttcta aacataattc ttcggtttga ttttttacat
    10141 actctaatgc cttgtaattc tgccttattg ctaaaataca cataccatca gtttgatttt
    10201 ctatattttt taattcacga taatcgtatt ttaccctttc catattttca atatattcat
    10261 tagtgatata ctttaaagaa tctcctaatt ttgtatcata aataccaata tgaatatctt
    10321 tccaattact attaaaataa taccctttat catctaaaaa taattttttt atttttttag
    10381 ataaatgcat gaatatacac ccctatcatt attaaattag ttattattaa aagtttgatt
    10441 attaatcttt atagtttttt aataataact ttattttttg tttattataa attcgctctg
    10501 tatattgtag ataccttttt catattttaa tagttaatcg ttttaattct tcctttattt
    10561 taaaatttaa atttgtctaa atatttctta ttttatatac attttttgat aaattaataa
    10621 agccataata tatttaatat atcaatcaca atataggact gaagttataa ataaattatg
    10681 gctattcaat acatttaatt ttatattttc ttgtctaatt atttctttat tatatttccc
    10741 atttttttat aaagtggcaa tgttactaaa gatattataa cagccttcag cacattaaat
    10801 ggagctatca tccaaattac aaaagtgaat aaatcatgaa cttgaggatt tatagctgaa
    10861 cctaaaccta ttatagcatc tagtcccata agttgaccat aaagtggcaa cattacaaaa
    10921 taatttacta atgaaccaat tatagtcata gatattgttc ctaatacaaa accaatcagt
    10981 acacctttta aatctccact tctcttatat gaataacaaa gtatcattac ataagaacca
    11041 ccaattaata tatttgcaaa ttctcctatt cctccagttg ttgttgctgt tattgcttgt
    11101 aacacatttt ttactacaac tattccaaat ccaactaatg gtcctagaga cattcctccg
    11161 aatattgctg gaatatctga taagtcaatt tttaagaaat ctggaaatat acctggtatt
    11221 ggtgcttgaa taaacattaa taaataagat attgcagata aaattgacat tactactaaa
    11281 gttcttgttg aaatcatatt gcctcttttt gctgtgtttt gcataattat tttcccccta
    11341 atcaatatgt ttatattttt taggaaaaac aaaaaactcg aagaccctat cttcgagttt
    11401 ttatggatat agtttaaaca aactaaaata aacttattta tcttccataa atatcttctc
    11461 tcatccagac tttactgtcg gctttggaat ctcaccaaat caaccaccta agtagttcgc
    11521 gggctatacc gccggtaggg aatttcaccc tgccccgaag atcttctttg attttttaat
    11581 tttccttaca ctcttatatt agttaatttt ttaataattg tcaagttatt ttttctcata
    11641 cactactttg ccatttatta tagtgtaaag tacattactt tgaatctcta gtggagaatt
    11701 atcccaaatt actaggtctg catctttacc aacttctata gagcctactc tatcttctat
    11761 tcctaaagcc ttagctggat ttatcgtaat tgattctatt gctttttctt ctttcatacc
    11821 atgttttact gcaatacctg cacatattgg aagatattga actggtatta ctggatggtc
    11881 tgtcattata catacatcaa gacctgcatt tgaaagtatt cctgctgtat taaatgtcaa
    11941 atttctaagc tcaaatttag acctttctga caaagatggc cctactatta caggaaaccc
    12001 ttcttccact aattcatcta caataagatg accttctgta caatggtcta atgtaagctt
    12061 taaattaaat tcttttgcaa ttcttattgc tgtaaacata tcatctgctc tatgagcatg
    12121 tgccttaaaa ggaatttctc ttcttaaaac tggtattaaa ctttccattt ttatatcata
    12181 ttcaggtttt tcacagtctt catcatcatg actctcatac atatcaatat cctctaaata
    12241 ttcttctgct ttttttaggt tttctctaag taaagcagct atagccattc tagtttgagg
    12301 agatttatca tcctgaccat aacagctctt tggattttct ccaaaagcaa ttttagatgc
    12361 aactggattt tttattacca ttttatcaat tctttttcca catgtcttta tagctataca
    12421 ttgaccaccc ataacatttg cacttccagg ggttgtacaa acagaagtta ttccaccttc
    12481 aaaagcttct ttaaaagttc tatccattgg attaattcca tctataggat ttaattgtgg
    12541 tgttattggg tcagtctctt catttccatc tgctccttca aaccctatac catcttccca
    12601 taaacctaag tgagtatgag catctataaa tccaggaaac acaagtttat tactagcatc
    12661 tattacctct acatctaaag gagctatcaa atcttctcca atttctatta tttttccttc
    12721 atctataagt atatctccat gaattatccc atttgttatg gtatttattt taccattttt
    12781 tataaaaatc attcattttc ctcctgattt tcaagactat cgttcttatt ttaatatatt
    12841 ttcttactat atacttttac aaatttttaa catttataca ctcgagtaat cttatttaca
    12901 aatatagaat ctaagtacta taaccataca taaacttatc tattttttca ttgataaggc
    12961 aactcttttt ttgtttaagt caattccaat gacttttaca tctactatat cacctactgt
    13021 aacaatactc atagggtctt tcacaaaact attactcatt tctgatttat gaactaatcc
    13081 gtcatttttt attccaatat ctacaaatgc accaaagtct acaacatttc ttattgtacc
    13141 ttttaaagtc ataccctctt gtatatcttc aatttttaag acatcagttc taagtatagg
    13201 ttttatacct tcttctcttg ggtctcttcc tggttttttg atttcagcta tgatatcttt
    13261 taacgtaact tgtcctactt ccagtttttc acttaattcc ctcaagccaa tttcttttat
    13321 tttttcatct atttcaccta tattcttatt ctttacatca tctaaagaat atccaattat
    13381 ctcaatcatt tttttacata tatcataact ttcaggatgc acacctgtat tatctagtgg
    13441 atttttaccc tctaagattc tcataaatcc agcacattga gtaaatgctt gaggtcctag
    13501 tctttttact tttttcaatt gtgctcttga cgtaaaatct ccattttctt ctctatatgc
    13561 tattatattt tttgctatag ctttacttat tcctgcaaca tgttctagta gggagtatga
    13621 tgcagtattt aaatctactc caacactatt tacagaatct tcaacaactc catctagtac
    13681 ttcttctaat ctctttttgt ttaaatcatg ctgatattga ccaacaccaa tacttttagg
    13741 gtctattttt acaagctctg ctaatgggtc ttgtaatctt ctagctatag atatagctcc
    13801 tcttattgat acatttatat ctggatgttc ctcatttgca agttcagatg ctgaataaac
    13861 agatgctcct gcttcactaa ctatcacata ctgcacttcg ctatctattt cttttatcat
    13921 ttctgataca aaagtttctg attctctaga tgcagtacca tttccaattg aaattatatc
    13981 tatatcgtat ttttcaatca atccttttaa aactttcttt gctccctcta catcattttg
    14041 agggtctgtt ggatatactg ttgtataatc tagtagttta ccatttttat ctactacagc
    14101 tattttacat ccagttctaa atgcagggtc aaatcccata actactttgt ctttaactgg
    14161 tggttgaagc aataaacttt taacattctt tccaaataca cttatagctc tttcttgagc
    14221 tatttctgtt aaatgatttc ttatttctct ttctattgat gggaaaatta atcttttata
    14281 agaatcttct attgaactta cgatttcttc tttatttttg aaatttttat catttacata
    14341 ttcgtttatt atgtaattta aaactttatc attatttatt tccaacttaa cctttaggaa
    14401 gctttctttt tctcctctgt ttattgccaa aactctatgt ggagccatac tttttactgc
    14461 ttcgctataa tcataataca tatcataaac cgatttctca tcagtagcac ttttgctaac
    14521 tatcatacct tctcttaaag ctaattctct tatatacttt ctaatcttag catcatctga
    14581 gactagttct gcaattatat ctctagcacc ttttagagca tcttcaatag aaagaacttc
    14641 tttctcttca tcaagataat tctttgcctc tatttctata ttatctaaat tattctctag
    14701 tattgacaat gctaaatttt ctaatccttt ttcttttgct attgttgctc tagttctttt
    14761 cttttgttta tatggagcat atatatcttc tacttcttgt aatgtttttg ctttttctat
    14821 attttgtgtt atttcatctg ttaatttccc ttgttcatct attagtctaa ctacatcatc
    14881 ttttctactt tgaagatttc tcaaatacat taatttttca taaaattctc ttagtgtaac
    14941 atcactcatt tcgccagtca tttcttttct atatctagct ataaatggta ttgtatttcc
    15001 ttcatcaata agttttaatg tattatttat ttgttcatct ctaagattaa actctttttt
    15061 taagatttga tttatatcca atttattcac tcctaatgtt agtttttcat aagtataaca
    15121 aaacagaacg aaggctgagg gttatcgtcc tcagcctaag tgctgtaagt gcatattaag
    15181 cactaacctt cttatacata tatcttaatt acagtgcata tttattaaac tattttattc
    15241 atttttaagt attcattaat aaacaagtct atatttccat tcattacact atctacattt
    15301 ccaaactcag catttgttct atggtcttta accaatttat atggttgaaa aacatatgac
    15361 ctaatttgac taccccaagt tatttgtgag tatttgcctt gtatatcttc tattttttcc
    15421 ttttgctcta actcttttaa ttctatcaat ttcgccatta aaagtctcat agctctgtcc
    15481 ttatttagat gctgagacct ttcattttgg cactgaacta ctacaccagt agggatatgt
    15541 gttattctaa ctgctgaatc agtcgtattt acatgttgcc ctccagctcc agaagctcta
    15601 tatgtgtcta tctttaaatc tgatggatta atttctactt ctatattttc atctaactct
    15661 ggtgttacat ctatagatgc aaatgaagta tgtctttttc ctgatgggtc aaatggagat
    15721 attctcacta atctatgaac tcctttttca ctctttaaat acccataagc atttgttcct
    15781 tctacaagaa ttgtagctgt ctttatccct gcttcagggt ctgatattat atctaaaagc
    15841 ttaactttat aacccttact ttctgaccat cttatataca ttctcaaaag catttgagcc
    15901 caatcttgag catctaagcc accagtacca gcatttatag ataatatagc attattttta
    15961 tcatactctc catttagaag agtctttatt ttcatttcag ataatttttc ttccattgat
    16021 tctatggact tttctatatc tctttctaca gaatcatcat cttcttctaa acctatttca
    16081 attaatactt ctatatcttc taataagctt tttaagcttt catattcttc taatgtctct
    16141 tttaaagatt tattttcttg aagtactctt tgagcgactt cattatcatt ccaaaaatct
    16201 tgttgattca tcttttcttc attaatttgt atatttttca tcaatgaagc tatgtcaaag
    16261 agaaaccctc aattcttcta aatttgaagc catctgctcc atactatgtc tatactcttg
    16321 taactttaac atttaatcct ccgagattat cttccacaac agtttttata ttttttacca
    16381 cttccacaag ggcataagtc atttcttcca accttttcat cttttttaac agttttactt
    16441 gttggaagtg ttccatctga tggagaagat agattttcaa catctacaac tgcttttctt
    16501 tccactggag tttctattgt tatattaaat aaatatctta cagtatcttc tttgatatgt
    16561 ttattcattt catcaaacat atcaaaacct tccattttat aagcaataac tgggtcttgc
    16621 tgacctacag cacgaagacc aataccttgg cgtaattggt ccatagcatc tatatggtct
    16681 atccaatggt tatcaacagc ttgaagtaag attactctct caacttctct cattctctca
    16741 tagccaactt gttcttcttt tgaagtatat atcttcatag ctatttcata aactttttct
    16801 gttatttcaa ctggattaag tttttctatt tctggtattt ctatgctacc ttttggcaag
    16861 aacagattat acatatgttc tttgaatcct tcttcatcaa atcctttatc ttgtgtataa
    16921 agagtaactg cttcttctat tatagaatga gtcattgatt gtatttgttc ttgtaaatct
    16981 tcaccttcta atacacgttt tctctctgca tatattattt ctctttgttt attcataaca
    17041 tcatcatatt gaagtacatg ctttcttatt ccaaagtttt taccttcgac tttcttttgt
    17101 gctccttcaa tagacttaga taatattcta tgttctatcg gcatgtcttc ttcaagacct
    17161 attttatcta caataccaga tattctatca ctaccaaaca atctcataag gtcatcatca
    17221 agacctatat agaatcttga actccctggg tcaccttgac gtccagcacg acctctcaac
    17281 tggttatcta ttcttcgaga ttcgtgtctt tctgtaccta ttatagcaag tccacctgct
    17341 tctacaactt gtttttgttc ttcttgtgtt tgttgcttaa acttttcaaa tagtttttca
    17401 tactcttctc tagcttcaaa tagaatttca ttacctttta caggtatacc ttctattgga
    17461 gtatctactc tatttactat ttcttcttta aatccatttc ttctcatttc tctcttagtt
    17521 aaaaagtctg gatttccacc tagaactata tctgtaccac gaccagccat attagtagct
    17581 attgtaacag ctccaagtct accagcttgt gctattattt ctgcctcttt atcatgatgc
    17641 ttagcattta atacctcgtg ctttatgccc tttttcttta gtatttgtga taataattct
    17701 gatttttcta ttgatactgt acctacaagt attggttgat tgactttgtg cctttcaatt
    17761 atttcttgtg ctacagcatt aaatttacct atttcacttt tgtatacaca gtctggtaag
    17821 tcttccctta tcataagctt attagtaggt acttggaaaa catccatttt gtagattgct
    17881 ttaaactctt cttcttctgt ttttgctgta ccagtcatcc cagaaagttt tttgtacatt
    17941 ctaaagtaat tttggaatgt tacagtagct agtgttttag attctctttg tattttaaga
    18001 ccttctttag cctctattgc ttgatgtaaa ccttctgaat atcttctacc aaacataagt
    18061 ctacctgtga actcatctac tattactatt tctccatctt tagcaacata atcaacatct
    18121 ttcttcatta ttacatgtgc tcttaaagct tgatttatat gatggtataa ttctgtatga
    18181 gatatatctg ttatattatc aacattaaaa tacacttctg ctttttgtat acccgatgct
    18241 gttagagata cagccttatc tttttcttct aattcataat catctggttt taaggttaaa
    18301 acaaatgtgt ttgcatctga atataagtga gttgatttgt ctccaggtcc agatatgata
    18361 agtggtgttc tagcctcatc tattagtata gagtcaacct catccactat agcataattt
    18421 aatcctcttt gaactctttg ctctttgtgt attaccatat tgtcctttag atagtcaaat
    18481 ccatattcat tatttgtacc ataagttata tcacaatcat attgttcttt tctaactttt
    18541 ggattttgac cgtgtataat aacgcctaca ctcattccta aaaactcata gatttttgcc
    18601 atttggtctc tatcacgttt tgcaaggtaa tcatttactg ttacaacatg tacacctttt
    18661 ccagtaagcg cattcaaata aacaggagca gttgcaacca aagttttacc ttcacctgtt
    18721 ttcatttctg ctattcttcc ttgatgtagt actatacctc caatcatttg aactctgtag
    18781 tgtctaagtc ctaacactct cttagaaact tctctaacta ctgcaaaagc ttctggaagt
    18841 atatcatcta tactttcacc atttgctaat ctttctttaa atatatttgt catattttta
    18901 agttccgagt ctgccataga ttcaaattta ggttctaatg aatctattat atcaacagtt
    18961 ttattgaact tttttaattc ttttttatct gccatgttga ataaattatc cataaagctc
    19021 atattttaca tctctcctcg ttctttattg tactattatt cattttacca taaaaactat
    19081 acttttaata ttaatttaaa aagctatcga taatttattt atcgatagct aatatgatta
    19141 aatctaaatt gctttattca tgttctatta taccatatcc accattatta cgcttataaa
    19201 ctactgctat ttcatctgaa tctatgttct tgaacatata aaaatcatgt cctattaatt
    19261 ccatctgtaa tacagcttct tcttcactca ttggcttaac actaaatttc ttacgtcttt
    19321 gaattactaa ttcttgattt tcatccccat aattctcttg ttcatcagct agatttatct
    19381 ctgcatcttg gaatcttata ctcttgttgt ctttatgttt atcttgtaat ctctttttat
    19441 atttttttaa ttgtttactt aatttatcat atacaacatc tatagctgtg tataaatttt
    19501 cctgcatatc ttctgctcta attataggac catttacagt agctattgtt acttctattt
    19561 tttgtcttgc ttttctcgca cttactgtaa cttttacatc agtgtctgga tgaatgtaat
    19621 tatctaactt acttagttta gtttcgattt tattttttat tgcatcagtt aattcgattt
    19681 gttttccaga tataattatg ttcataaata catctccctt caattatgcc agtataagaa
    19741 aatttatact ttaataatat attctacacg aactctcata atcctctttt tattatttac
    19801 ttttatgatt ttttctattc aattgataga attatttatc atttttagtt acattgatac
    19861 ttatgtggat taatattatt acaaacacct gtggataatg tggataaaac tgttaataac
    19921 tcttgttttt gtttatttaa agctattttt actcctattt ctatccacaa aaatctccat
    19981 taattgttta ttaatatgtg aattcatgtc ataagatggt aattttagga aattatgtgt
    20041 attatatgcc aatatacctt tataattttt taataaatat aaaccctcta aaacctccat
    20101 aatctatctt ttctttcatt atttatattt aaaactaatt aaaagttata aagcatactc
    20161 aacaataatt tcatatttaa tacctattaa cattcatata cccaaattat aattaactta
    20221 ctctctttat ttaaactaag tattttatat tcatatacta tcttgaaatt atatttacga
    20281 gttgtttaag cttttgtcta tctatatctt caaaaggact gttatttatt ctaatcgctg
    20341 taccaaaatg aaaattactt gccttagtta attcctttat cttttctata ttattaaaat
    20401 taagacctcc acctaaaaga atttttatgt gattagaact aagcatcata ttctttatca
    20461 tttgtatgtt atcaactata cttcctttcc ctcctgatgt taatatattc gtaattttat
    20521 cataatcctt caaaatttta acactatcta ttgtatttga ttcatctatt gctctatgaa
    20581 atgtaacatc aagattatca caacatttca ataaaacatt tagatttttc tcatctatac
    20641 tattgttttt atccaataca ccaaatacaa ctccattagc accaatctct ttaaccacac
    20701 ttatatcttt ttgcattata cttatatctt ctttactgta tacaaaactt ttcgcatgat
    20761 gtcttatcat tacgttcaca ggtatcttta cactgttaac aacactttct ataagaccaa
    20821 aacttggtgt caatccacct tcagttaaag cactaactaa ctctattcta tctgcaccac
    20881 aatcttctat aattttagcg tcttctactg acattcctat tatttctaac ataactactc
    20941 ctttgctaat tcatatattg catttgcaaa tatctttgca tttaatatta tatcttctat
    21001 atccatgtat tcatcaggat tatgagctat tcctttttgt cctggaaatg aaggtccaaa
    21061 cggaactata tttggcatta cttttgcata cgtacctcca ttagttgtta cgggagttcc
    21121 atctaatgat gtaacccttt cataagctag ctgaagcttt ttaacaagat tactctcttt
    21181 atcaaaatat acaggattaa aattagaaat tacctctata tcgatactat ttggaaaact
    21241 agcttttatt tcatccaata catctttcaa tttataacta aaatcgtatt ttacctttat
    21301 atctaatata gtatcactat tttctatatt cagctcttta tatgtaacaa gcacattttc
    21361 agacatattt tctataaaac ctaggtatac atcttcttca taattgattt tactttttat
    21421 tctaatcttt gccatgcctt tttcaccata tacaacagga tatttacaat ctggtgtaaa
    21481 tcccataata ggtgcttttt ctttttctaa ataatatggt atatcttcaa atccactttc
    21541 ttcatttgta ccaaatatta ttctaactct cttatctaat tttaagttta attctttaat
    21601 agcatataat ccatataagg cagacattat aggtccttta ttgtctagta ctcctcgtcc
    21661 ataaattctt ccattagttt cttctccctt ataaggctga tgcttccaac catctccttc
    21721 atgtacaaca tctacatgtc caattacaca aacataatca tcactatctc catgctctgc
    21781 ataaccaata taattatcca agtttctaac cttaaatccc aatttttctg atatttctaa
    21841 tgctttatta agtgcctccc ctactttttc gccaaaaggg aagccatttt ctgactcccc
    21901 tttgacacta ggtattttta caatatctat aatatcactt aataaatctt cttttaactc
    21961 atctatttta ttacttatga tattcataaa aatctcctta ttataaaact ctattttaat
    22021 tatgtaacca ctactttact cacttacttc tattggtgct tttattctag cctcatatgc
    22081 ttccatccac tcatttgata taaccttatg tatagtcttt ccatctactc tatttgaaat
    22141 atatacagta ggagattctt ctttagttac tactgaaaaa gtaaaatcag ctatatttgt
    22201 agctactccc cattcacctt tattattcat ggcaactaat gataaatcac cagcttttcc
    22261 tcttcttttg ataagctgtt tatcaagttc atttactgca atatcacatg cttcttgtgg
    22321 atgcttccct tctttcataa gtctaactat ctcataagat atacatccct tcattaaatc
    22381 ttctcccaaa ccagtagctg tagctcctcc tatactacta tcaacataga atcctgaacc
    22441 agatagtgca gaatctccta ttctacctct tttcttcata aacaatccac ttgttgatgt
    22501 agctgcacac atttttccag ttttgtctaa agatatcata ccaacagtat catgaccagc
    22561 atatgggctt aatcctttgt ctaaagtctc tttttttctc tttttataat gtaacttcgc
    22621 tctatcagtc aacatatttt ttctttcaaa tccattttta tgagcataat cttcagcacc
    22681 tattccaact aaaaaattat ttaccttctc ataacttaac ttcctagcta tgctgacagg
    22741 attagaataa tctcttatac cagcaactgc gccaatatct aatgtgttac catccataaa
    22801 tgcagcgtca agttctacct cacaattttc atttggaagt ccaccatatc caaccgattt
    22861 atagaatgga tagtcttcaa ctcttcttac agcaagctct acagcatcac ctgcttccat
    22921 gcctttttct aaatgttctg atgcctcagt tattccttct agggccattc tccatgtcgc
    22981 tatcattccc cacatgttaa aattcccctc cttaaattat atagatttta ctaatgtatg
    23041 cttattataa atagtataat atttaaactt gctaataatt aatactctta ttgataacaa
    23101 aactcttctt tcatcaaagt tatatcttct ttatttgcta ttgcttttgc caatttacat
    23161 atgttattta atgaatcatt aagaccaatt ccaccttttt ttggagtctt aactatacat
    23221 atgtcatttt tatattcgtt tatcacatct atattttctt ctgacatagc tgcaaaagct
    23281 ggtatatctg ttttatcatt tatattttta gacagtattg ctgacatctt agaaaagttt
    23341 tcgtaattaa agcttggtcc acagattact acatcagggt ttaatttttt taccatagct
    23401 atcatcttac tcgttacttc ttcttgatta tttacaaaaa attcatcccc acaaaataag
    23461 gtagctatta ccttagattc atttaaaaat tgctccatca ttacacctgg tcctaatggg
    23521 cttgattttc cagcaggagg tatattactt ttctcttttc ctccagctcc agcttgtatt
    23581 tggtccaata tcataactat tttcttcata atcttcctcc tataatacta aatcatcaaa
    23641 agatatatca tcatcttcct cttcaacagt ttcattttct tctctcaagt attgcttatc
    23701 tagcattttt ataaatggta tatacataaa tattcctagt ataagaagta ctaattgtaa
    23761 gactgcacct tgccatcctg ttgttaaaaa accagagaat ataattggag ttgtccacgg
    23821 taactgaact ccatttgtaa gaggcactaa tcctgctgac atacagaagt aagctattat
    23881 tatatttatt gttggaacta ttgcaaatgg tatcaatagt attgggttaa gcattattgg
    23941 tagaccaaat atcaatggct catttatacc aaatattcct ggtaaaattg atagttttcc
    24001 cattgacttt atacgtttac ttttacatat taataacata gctatgacta atgatagtgt
    24061 actacctgct ccaccaaatg tcgcaaacat atcttggaat tgacctgtta ttatgtttgg
    24121 tatttttgaa ccagcttgaa aagcagctaa gttttcagct gatagaattt ttaatattgg
    24181 attatacaca gccccaacaa cactacttcc atttattcca aagaaccaga ataaatgtaa
    24241 gaatatataa gcgattgcca tagcacctaa actatttcct aattttaata atggcatttg
    24301 taaaaactta aatataaatt catgtatact tccatatggt gtagcttcaa aagctatctt
    24361 aactaaaaag aaaactacca taactattgc acttggtatt aatgctgcaa atgattttga
    24421 tacagttgga ggaactccat ctggcatttt caatgtccat ccttttttta caactgcagc
    24481 aaataatttt acagaggtta ttgctgttat catacctaca aacattccct tagaacccat
    24541 ccaacctagt ggtatacctg ttaccatgta aactgattct gttccttctg gtgtatatgg
    24601 tattacaaat ggtgttatta taaaaaaggc aactattgca actgctgctg atgctattgg
    24661 gtctacatct atttgctttg cataagaata tccaatccct aaaactgcaa gtagagtcat
    24721 tacttcaaat gttgctacag ttggttgttg caattttaca gcccaatctg gaccaaaaat
    24781 ttgacctaca aattcattcc atccttttat aggaaaattt gctactaata aaaataatga
    24841 acctattatt aataatggtg atgatactaa aaaaccatct ctaattgcaa ttaataattt
    24901 atttttacct attttttcag ccaatggcat aagtattctt tccaatctat tcatctatcc
    24961 accctcattt cctattattt tttactataa ttctacgctt gtttagattt tattagcttt
    25021 atagcaagtt ttaatacttt ttccccattc atagttccat aatcaacttc attgattata
    25081 tctataagtt ttccttgagc ttcaaacttt ggttttaact cctttagcat gtatttaact
    25141 tgtggtccta ataatataca atctggattt ttttcttcaa ctattttgtc aatttctgct
    25201 atcggaaatg cctctacttc tattggtaaa ttgtgttcat ctgctacctt ctgcatttta
    25261 tctgcaagta aacttgttga cataccaaag ctacaaaata aatatacttt tcttttcatt
    25321 ttttaaatcc cccttaattt ttttatttta atttttgaat ttcttttctt aacataatca
    25381 tttcttttat aaggtttctc tcactcatac aagtcattaa atggtcttga gagtgaatca
    25441 ataaaataga tatatctgat ttatctcccc cagcttcctt ttgtataagt tcagtttgta
    25501 ctttatgtgc ttttaaaatt tcttcatttg cattttcaat taattcatct gcttttttaa
    25561 aatcgttttc ctttgcatag tcaagtgctt cataaagcat agattttgca tttccagcat
    25621 gaattataat ctttaaaact atttcttcca ttttttcctc ctaagttgct tctctacttt
    25681 tagattattt ataccttaca aacatataat atcttataaa aacaaaaaaa catgcacaac
    25741 tttttccatc acatggaaaa ttttgtgctt atccatagtt cactatgaat tttatataag
    25801 aatatctttt accttttcaa tgtcacattt acttagaata tctagcatat tttcatcctc
    25861 tgttaaatta gatatttctt taaataattc tctaaatact tttctatcat catctttttt
    25921 atacgctaac aaaagtataa tctttatatc ctttccattt ctaattttta ttggtgtttt
    25981 taaccttgca aaacataaaa cagattgttt tatatatttc atgtctccat gagggaataa
    26041 tattcctttt ccaacattgg ttggatatga cttttctctt gttataatag attgtaaata
    26101 ctctttttct gcataatgtt tttcaattaa taaattagtt aaatatgtta tagcttcata
    26161 aatatcatca tgttctaact ctcttattaa ataatcagac atcattaatc tgttcataag
    26221 tttatatgaa gtgtcttttt cttttatttt gtttgtgaac tctttaagtt tattctcatc
    26281 ctcatcagta aataatggtg ttgttataaa aattggtttt tggatatttt catactcttg
    26341 aaatgcaatt ataaaatcta tagtcttatt aaagttatct ctttttaaat cttcctctct
    26401 taatgtttca ataattttta tgttatcaaa tctcttttct atctttactt ttaatagcat
    26461 acttactcca aaactaaatg gacatacaat acttgccttt ttatttgatt cccttttgct
    26521 gtattttctt tctagagagg tttgaaagtg aagtgtaaga taaccaattt catcttccgt
    26581 tatttgtttt ccttttaatt caatttctat tgaacttgat attactgtaa ataaaaaaca
    26641 aaatttagtt tttatgttat ctaaaagtgg attttctaag taaacattgc ttttcatttg
    26701 atgcattgta acattcaaat gacatattaa ttgttcataa agtaatatat cattattaaa
    26761 atttattcct gattcttctg atactaaatt tataattttt tttgtataac ttgctaattc
    26821 ttcatttaga tataaagatt tcttaatatt attactattt atttgtttgt tcatacccaa
    26881 tattaaagat tctataaaca atttttcttc ataatttaat actatggata aattagattt
    26941 caattcatct tctatatcca atataagcat ttttagctta agctctttct tagtatttga
    27001 tacttttaaa gaatttccca gccttattcg tattatattt ataagcaaaa atagcatcaa
    27061 ttgcttgaat gatatatctg taagtcttat atccatatcc tcttctatag taagcattat
    27121 gttttttaca attattatat ctactatatg gaagtactct atttcatcta tgtttaatct
    27181 gtatttatct atatatttaa ataagaatga tactaacatt attcttatgt ctttctcatc
    27241 cccttcaata tatataccac tgcctttttt agtcgagata tttaaattat aattatttaa
    27301 tgctactgat aatttactta tttcttctct gacaatattt ttatttatgt acaacttatt
    27361 agataaatca tttaaagtaa cttttttatt attacaaagt agtaaattta gtatctcaac
    27421 ttgcttatga agctcactat ttactctttt aacttcacct cgcaacttct gatttaccag
    27481 tattttgtct gttccatctc cactaaattt aatcccaata ttaggttttc ttttgatata
    27541 agcattacaa aaagtagata accattcatc aatatatttg caatcatttc ttattgtcct
    27601 ttctgaacac tcaaaatcat tggcaatctt attaacattt ataaactctt cttttaatag
    27661 caatgttgtt aataaatttt cttgtctttt attcaaacat atcacctctt tatgactcaa
    27721 cctaaattta ttatatataa aaaagagcta tacataaagg tatgctagct ctcttttaat
    27781 atgatattta gctgacctgg tttttgaccc cacactcgcc tactggaggg ttcgcttggg
    27841 ttcgccgcac tactactctc tctcgatttt attatcggta ggtcttactt ctaagccgca
    27901 ccatgatcat taacccaatt tttagtgtta acttacgtgg atccctttgc ctgcgactgg
    27961 cttggacttt caaccacaga cctaaatatc acttattcat tttaatattt taaactaaaa
    28021 ttgtcaatat ataagattat tccataatat aattatcatt tgcttttgta agtagagtca
    28081 atacaaaaac cttattagca cctgataact ttaaaagttt agaaatttcg tttgttgtta
    28141 gaccagtagt aaatatatca tctattaaaa gtatattttt attatttatt aatttaacat
    28201 tttctttaac aacaaataca ttttttaatt cttcttttcg ttctttctta ttcaatttat
    28261 atagcattct tgtatatttt tttctactta tgcaatctaa taatggaata tttactattt
    28321 tacttaaatt aaatgctatt ttttgtgcct gattaaaacc tcttttattt agcctttttt
    28381 tatgaagtgg aacaaataat atataatcaa attttatgtt ttctaaatat aatttttctt
    28441 tcattatttg agctatatac ttagccataa aggttttttg gttatacttc agccctaaca
    28501 ccatcttttt tgtcacatca ttgtattcaa tacaagatat tgatttatca aaataaaagt
    28561 ctttattaaa acaataacta cactcttcaa taaattcttt ttcaattgaa tgacgtatta
    28621 ttggttttcc acacttcatg caaccatctt gtataaaatt catttcttta aaacaacttt
    28681 tacatattga ataagtattt gttttcttta tggatttatc acaaattatg caacttatat
    28741 tctcaggata tataaaatcc aaacatttat taatcatatt tttaaaattc ttaataaaat
    28801 tatcattgca tattttcata tttattctat caacaaccct tcttgtttaa atttatttag
    28861 cttatatcct aaatttgaat acctttgatt aactctatta ttttttatca tatattcaag
    28921 atatttcaca tctccaacta atacaactaa tttttttgct cttgttactg ctgtatataa
    28981 taaattacgg ctaagtaaca taggaggagc ccacgctatt ggcaatacta caactggaaa
    29041 ttcacttccc tggcttttat gtattgttgt acaaaaacta tgatcaattt catctaattc
    29101 atcatatagg tatgatacaa ttttagtctg gtcaaataat atataaatag ttttcttatc
    29161 tttatctatg tgataaacat atcctatatc tccattatag ataccttctc cactttcttt
    29221 ttggtcttct gtttcccatt tttttgtata attatttttt acttgcatta ccttatcgcc
    29281 aactctaaat aatcttttag aaaaattttc ttcaacttta aatttttctt ttttgttaag
    29341 atacttctgt aactctatat ttagattagt aactcccaat tcaccttttc tcattgaaga
    29401 taaaacttgt atatctttta atttatcaac tttataaaat ttaggtaatc tttcattaac
    29461 caatcctatt atttcattta aaatttcttc attagttgat tttcttataa aaaagaaatc
    29521 tttacctttt gtatttagat aaagtggttc tccattgttt atcttatggg catttacaat
    29581 tatcatactt tcttgcgctt gtctaaatat ttcatttaat ttaactacat ttatgatatt
    29641 agaattaatc atatccttaa gtacatttcc agcacctaca gaaggtaatt ggtcactatc
    29701 tccaactaaa attactcttg ttcctaactt tatagctcta agtaaattgt acattaagat
    29761 aatgtctacc attgatacct catcaacaat tattacatct gaatttattg ggtcttcttc
    29821 atccttaaaa aaagttaaat catcatctgt tgaaaatccc atttctagca acctatgtat
    29881 agtttttgct tctttattag aagtttcact cattctcttt gctgctcttc ccgttggagc
    29941 tgccagagtc acacttttac cattattttc aaatatatct atgatagcat taatggtagt
    30001 tgttttacca gttcctggtc caccagttat aattaatact ccactattaa cagattcttt
    30061 gactgctaaa atttgttttt ctgctaactt aattttctta tcttcttcta aaacattaat
    30121 ctcgttatct atatctattt ttaagtcttc aaattcatac tgtgataatt ttactatttg
    30181 actacatact ccattttcag ctaaatagta tggcataagg tatataagat tttcattgtt
    30241 tactttctct atatgtattt tttgattata aactagcatc attacacact cttttattat
    30301 ttctccattt agctccaata atttttccga atcttgtatt agaacatgct ctggtaaata
    30361 tgtatgccca ttacttaaag atttatttaa agtgtataag attccctgac aaattctatc
    30421 tttagagttt ttatctatac caattttact tgctatacta tcagctactt taaatccaat
    30481 tcctcttata tcttcagcaa gttggtatgg gtttttattt ataacttcta ttgcactgct
    30541 tttatatttt ttatatattt ttaggcagta atttggtgtt attccaaaag gagatagctg
    30601 tattattata tttctcaatt ctctgtcttc ttcataactt tttactattt gttttacttt
    30661 tttactccct ataccttcta cttcttgtag tttttctggt gaattttgta tcacctctaa
    30721 cgtatctaca ccaaacttat caattattct ttttgccatt ttttcaccaa taccatgtat
    30781 cattccagaa gataagtaaa cataaattcc ttctaaggat gatggtgtta ctggcataaa
    30841 actattcact tcaaattgag tcccataaat cttatgattt acccactttc cttcaacttc
    30901 tatgctttct ccaattgcta aagtaggcat acatcctaca accacaattt catcattttc
    30961 atttaccagg tgagctattg tataaccatt atcttcgttt ttaaagacta tttcacttat
    31021 cattccttct aatttttcca tttttatctc ctaaaaatac taataatcgc tccatttaaa
    31081 tgttagtata attttatata ttaaaactat gataatcgta gaaaagtttt atatttttat
    31141 acttaacgta atttccaact attatacatt ttatcagaaa caaaaaaaat ctgcatccat
    31201 aaaatatggt accatgaaat acaggtacca tatttaaata gctattttat tttaattact
    31261 ctatatttag agtataaatt aaatttcata agtatattac agtactaact tatttcagat
    31321 ttattctcct tgtacccctc agttttagta attcttctat aaagcataca tagtaaaatt
    31381 aaaattccca tgtaacctgt atatggatat agtgtaccta ctaacttatc aaatggcagt
    31441 aaacctccta tacaagctaa tatacttacc actattgtta taattttaaa cttagggtgg
    31501 tcatcttcta caaatcggtt tgttactgac cataacaatg gtacagctgt agtgtatatt
    31561 cctagtaaaa gtactactga gaataaaata cctattattg gagatatctt atcagctaag
    31621 taaagtgctg gtatctcttt tgtgtatata tttcctatgt ctgaaagtaa tgccaaattc
    31681 atcattatag ctgctgccat aagtgctact ccacctacta taccacccca tacagcatct
    31741 ttcttattta ctgcagaagc tcccattcct gtcaagaatg ttattactat tattacattg
    31801 taacaagtat aaagtactcc cgagaaatat ccatttgatg tagcagtttt aatatttaaa
    31861 ctatcaacca tatttccagc atttgataaa gcatcaatat tgctaaataa actaattgct
    31921 cctactaaaa gtgtaaatat tattattata ggtcctatat tacctagtat tttagaaagc
    31981 cttgtcaaac ctaatgaaac agttattagg gaaacgattg ccataccaat cctacctaca
    32041 tatggattta atccatagta ctctgataat gtagctcctg ctcctgcaat cattattact
    32101 aatgttccaa ataaaaacaa tggaccaaac cattcaaaaa atgttccaag atattttcca
    32161 caatatactt gataaatttt tattggctcc ttaagtttca attctcttcc cttatctata
    32221 acttctgctc caaaccatga aaatagtacc atagagatta ttccacctat aatacccgca
    32281 tatccataaa acgtaaaaaa ttgcagtatt tcttgaccag ttgcaaatcc agaacctatt
    32341 acagtagcaa cataagctcc agcaaaactt ataaccgttt taatatttac tttgcctgac
    32401 atacactact ccccccaatt aaaattattt ttaaagtgta agtttattaa gattatatta
    32461 gactcattat tgtgatattt caatatattt ttaattattt aacattagtt aacacattct
    32521 taaccatcct ttaacatata tcccataata tgtataaatt tagtagagta ttatataaaa
    32581 tgtattgacc agattaaata ctgtttattc attatatgat gataaattaa catttattaa
    32641 aaatatttaa ctcataataa aaaagctgtt agaaagtaat cgtaaaatta cattctaaca
    32701 gctttttagt taaaaattat tcacctaaag catcttttct taactgctca accttgtcag
    32761 ttctttccca aggtaaatct atatcagttc taccaaagtg accataagca gcaacctttt
    32821 tataaagagg ctttcttaaa tctaagtctc ttattatcgc tcccggtctt aaatcaaagt
    32881 gtttatttac taattcaaca agtttttctt ctgaaacctt gcctgtccca aaagtatcta
    32941 taaatataga taatggtcta gctataccta tagcatatgc aagttctatc tcacatttgt
    33001 ctgcaagacc agccgcaaca atattttttg caacatatct agctgcgtaa gcagcagatc
    33061 tatcaacttt tgtagggtct tttcctgaga aagctcctcc accatgtcta gaatatccac
    33121 cataagtatc tattattatt tttcttcctg taagacctgt atctccttgt ggtcctccta
    33181 taacgaatct tcctgttgga tttatataaa ttttagtctc ttcatcaagt aattctgctg
    33241 gtataacttc cttaataaca tgattaatta aatcttctct tattttatca tttgatactg
    33301 tttcacaatg ttgagctgat ataagaactg tatgtactct tacagcttta cttccttcat
    33361 attcaacagt aacttgagtt tttccatctg gtcttaaata atcaactaaa cctgtttttc
    33421 taacttctgt aagtcttctt gataatttat gtgccaaaga tatcggaagt ggcattaatt
    33481 ctggagtctc attacatgca aatccaaaca ttataccttg gtctccagct ccaacttttt
    33541 ctatttcttc ttctatctct tctccagtct tactttctag accctcatca actcccatag
    33601 ctatatctcc agattgttca tcaatagaag ttataactgc gcaagtatca caatcaaatc
    33661 catattttgc tcttgtatat ccaatttctc ttactgtttc tcttactaat ttaggaatgt
    33721 ctacataagc agatgtactt atttctcctg ctactaaaac taaaccagta gtaactgtag
    33781 tttcacaagc tactctagat tgtgggtctt tttctaataa tgcatctaat attgaatctg
    33841 atatttggtc acatatctta tcaggatgtc cttctgtaac tgattctgac gtaaataaat
    33901 gtcttgccat ttttattccc ccttatttat tcttaatttt tattataggt tttaccttgt
    33961 ttttaatttt agaaatttac tcaaaataaa aaaacctctc ctttataaga agaggttaat
    34021 aatcgtaatt acatactaat taactgacct catctttcaa gacttttgtc ttgtaggaat
    34081 tagcaccttt tcccatttgg gtggttgctg ggcttcacag ggcctatccc tcagcctctc
    34141 ttgataaggt aatcaagatt taagttttgt tttactttac tagcttacta tttttttaaa
    34201 attttgtcaa tatttttagt catatttttt ctattccttt ttttgtaaga caaataaaat
    34261 tttctatcta tctgaaattt aaagttaggt ctttttaatg ctttttattc atatattttt
    34321 ttgaaggggg tgttcctatt gtatttagct aaagtaaact acagcgatga tgatgttata
    34381 cagatgctaa gttcagttct aaaaactata attaatgaaa atacgattgt tgtttgcata
    34441 ggaacagaca gggctatagg tgatacttta ggccctttag ttggaacaat acttaagaac
    34501 agtaacttta aataccccgt ttacggtaca ctagataacc ctattcacgc cttaaatata
    34561 tatgagtctt tagatactat aaaaaataca catatacaag gtaacttctt ggctatagat
    34621 gcttgcttgg ggtcgcagag taacataggt aatatacaga ttagagaagg acctatcctg
    34681 cccggaaaag gtgttggtaa aaaactacct caaataggaa attattctat agtaggaatt
    34741 gtagataaaa ttgatgaaaa taataaactt tcatttaata atatacgcct tagctttata
    34801 ttagatttag ctgaaacaat agccttggct ttattagtat ccacctaatc taaatataaa
    34861 ctaaatgata aaattcttta tataaaaagg tagatatttt agagtatctg attaaatact
    34921 ccaaattatc taccttttta cttttatatt tcaagttttt atttttctac taaagcaaac
    34981 attaattcac ctttacagac aagttcttca cctacatatg ccttagcatc tgccttagct
    35041 atattttttc taagagttgt catttcaact tccatagtta atgtatctcc tggtcttact
    35101 tctctcttga ttctaacttt atctatacct gcaaatacac ctagttttcc tttgttttcc
    35161 tctacagaca tcattgccac tccacatact tgtgctagag cttctactat tagtactcct
    35221 ggcatcaaag gatattctgg aaagtgacct tggaaaaatg gttcattaac tgttatattc
    35281 tttataccaa cagctctttt cccaacttct agctctgtaa tcttgtctac taataaaaat
    35341 ggatatctat gtggtattaa tttttttatt tcatctatat ttaacattct aaactctcct
    35401 aattattttc ttttgaaaga cccaccagtt tcaagtaagt ctattgaacc taaagctatt
    35461 cctgtacctt ttgcaacaca agataatggt tcatctgcta ttgtaacagt tattcctgtc
    35521 ctctgttcta ttattttatc taatccatat aataatgaac ctcctccagt cattattata
    35581 ccgacatctc ctatatctgc tgctaactca ggaggcgttt tttccaaaac agcatgagta
    35641 gcaactacta tttgctcaac acattctcta agtgcttcca acatttctgt tgaatttata
    35701 gttatagaac taggaagtcc tgtaattaaa tttcttcctg ttatcttcat atatttttct
    35761 tcttctctct taaatgcaga acctatttca aacttgattt tctctgctgt tctttctcct
    35821 ataagaagat tgtgttgttt tttcatatat ttaactatag catcatcaaa tctatctcca
    35881 ccaaccttta tagattcact tacaacagct cctcctagag atattacagc tatatctaca
    35941 gtacctccac caatatctat aatcatatta ccattaggct gtgatatgtc tacaccagct
    36001 cctatggcag ctgctattgg ttcttctatg atatatactt ctctagctcc agcttgtctc
    36061 gtagcatctt ctactgctct tttttccact tctgtgacac ctgttggtac gcataccata
    36121 attcttggca taaaaaatct tccaaatccc tttttatcaa ctatcttatc tataaaagat
    36181 tttaacatct tctcagttac atcataatct gaaattacac catctcttaa aggtcttata
    36241 gcgactatat tacctggtgt tctccctatc atctttttag cttcttctcc cacagctaaa
    36301 acagaatctg ttcttttgtc tatcgccaca acagatggtt cttctaaaac gattccctta
    36361 ccactaacat aaacaagcac atttgcagta cctaaatcta tgcctatatc agctccagcc
    36421 attttttcaa ctcctttatt tataatatta gattatatta taagcctatt ttaatactcc
    36481 tataataaag catatacaaa aatctcgctt taatcaagag aaaataaatc ttttgagagt
    36541 attattattt tgctatttat aattacttaa tatatttctt tttataattt aattcaataa
    36601 aaaaagtagg tttttaacct acattttttt atgttccttc tcatatttta atttcgttgc
    36661 taatccccct ctaatatgtc tctctgattt atttttgtcc agcacatttt taacttcttt
    36721 ggcaagggat ggatttattt ccttcaatct ttctgtaaca tccttatgaa ttgtactttt
    36781 acttactcca aatgtcttgg cggtttgtct tactgtagtg tttttttcaa gtatatattt
    36841 tgctaccact atggcccttt cctctatatg agatctcaaa gattaccccc ctcacataat
    36901 agctttaatg atatatatgt aaaaacacca aaaaatataa caagcttaac caaatatgat
    36961 taaacttgtt atactattac ttaattagac tcattgggtc tatagatttt tctccactat
    37021 acgcctctaa atgcacatgt ataccttctt cactttctat ttgagaagta ctacctacag
    37081 tacctagaga ttgtccttct gttaccttct gttctttctt aacactaaca tttttatcta
    37141 gatttgaata tactactaca atgttattgt cactttttat tttgactgat tgtccatatt
    37201 cttcatcatc aaatacatct actactattc cgtttaatag agattttact tctttgcctt
    37261 tagtacaact aatatctaca cctttatgag tttcccaaac atcaagtgtt ttagaataac
    37321 taggctcttt ttctgaatat cctcttatta ctttatctcc aatgtaattt aattttgttg
    37381 tactcgattt attttcctct tttgacttag ctttttctaa gttttgtttt gaatccgttg
    37441 cagtagggat agcatctttc ttgtcttttt caattaaatg tatttcctct tcactatctt
    37501 tattagcatt ttccataatt cctttattag aagccaattt atctacatta ttatttgtaa
    37561 accaaactcc acctactgct aataaacaaa cacatacaaa caaagataaa taaaaaccat
    37621 ctttttctaa cagctttttc ttcattttgc acctccaaat atatttctac atttggatta
    37681 ttgacgtgtt tttggcattt tatacaatta ttagtatata tcttttattt ttgtatctgt
    37741 gtagtaatgt gataatatat cataatattt ataaccctct tctgccattc cttctgctcc
    37801 ccactggctc ataccaacac catgaccata tccttttacc acgaaatcta tgtatccttc
    37861 tccaaatttt atgtcaaagt ttgcagagtt caacttaaaa acagttctaa tgtcttttcc
    37921 agtcaattct ttatttccta attttatttt ttctacagtt ccggcatcac ttcttttagt
    37981 tattgaaact tgtttactta aattattcac atctatcaca atagtgctat atgcccttct
    38041 aagactttta acaaaatctg tgttacttat tttaagtgtt gacgcaaatt taggggaata
    38101 cttatcataa ggactttcca cagactttaa ataaggatat tttgcagaaa atacttcttc
    38161 actattttct gtttttcctg aagatgtaga aaaataaaga ggaagtatcg ccttatcatt
    38221 ataagttatt atttgacctt tagttcctct aactgcttct tggattttgg aatatttatt
    38281 cttaatccat tcttcaccat ttaatttttt aagagtatca taactcttat attcttgaca
    38341 atgtttataa tcagtacata ctactgcatt tttatgttta ctagatttac catgttcctg
    38401 tttatatact acatatgttc tagctgctac agattgagct tttaaagctt ctatatcaaa
    38461 ctctgaagac atctcaccag ctagtactcc acataaatag ttctccatgt ccatcttttc
    38521 ggttttccct gttatatgat tatatacatt gataattggt gcttttttat ttacagtttc
    38581 atagttcact gtattttttt cttctttatt acctttgtct tcaatatcac tttttttaat
    38641 agttttgttt atagatactg ggctctctgg tttttcagtt aattctacat ttttataaga
    38701 aactaaagtt ataagtgatg gaactaacac agagcaagtc acaaatccta acaaaacaac
    38761 caatgggttc ttcatatact ctcctcctta gcttttaaaa ccttgtattt aagtatatga
    38821 acaaccctat taatttataa ctttcttcta taaaccaatt attaatcttc tactatttcg
    38881 atttgaccac ctagtgctgt tatcttctta tctatatcaa cataacctct ttggatatgg
    38941 tatatttctc ctatttgagt ttctccttct gctataagac cgcataaaat aagggctgcc
    39001 cctgctctta aatcagtagc gtttactgct gcaccgtgta gctcatctac accattaaca
    39061 accgcacttc ttccatctat ttttatatta gcacccattc tattaaattc agcaacatgc
    39121 ataaatctgt tttcaaaaac agtttctata actacaccag ttccattggc tacagtaagc
    39181 atagccataa attgtgcttg aacatctgta ggaaatcctg gatgtggtaa agtttttata
    39241 tctattggtt ttaatacttt tggacctaca actcttacag aattgtccat ttctgttatt
    39301 tcacaaccag cttctcttaa tttagctaca actggtttta aatgctccat taaaacattt
    39361 tcaacagtta tatcaccttt tgtcatagct gctgcaacca tataagttgc tgcttctatt
    39421 ctgtctggaa ttacattatg ttctgctcct tttaactctt taacaccttt tatttttata
    39481 gtattagtac ctgcaccttt aacatctgct cccatttcgt tcaagaagtt tgctaaatct
    39541 actatttctg gctcttctgc tgcattctct attattgtag tcccttctgc taaagaagct
    39601 gccatcatta tgttttcagt tgctccaaca gatgggaaat ctaaatataa tttatttcct
    39661 actaactttt ctgttgctgc ttcaacaaaa ccatggtcca tttctatctt tgcacctaaa
    39721 gctttaaatc cttttaaatg taaatctata ggtcttgttc ctatagcaca tcctcctggc
    39781 atagaaattt tcgtactatt aaatctagca agcaatggtc ccataaccaa aaaagaagct
    39841 ctcatctttc ttacaagctc atatggagct tcacaagttt ttatattgct tgcatcaaca
    39901 gtaagtgtat tttctttata ttcaacttct gctcctacat gtcttaataa atctgatata
    39961 acatgaacat ctcttaaatt tggtactcca tttaatgttg attttccgtt tgctaataaa
    40021 gtcgctgcta ttattggtaa aactgcattt tttgcaccat ctattttaac actaccttta
    40081 agtggattac tcttttttac tattatctta gccatatttt ttgatctcct cataattaat
    40141 aatctaattt tataattggt gtggcaatat atatgagtgt ttttccgtca tcctcattat
    40201 atttaattcc tatatttaaa ttaatttttt cccctaaata ttctaattca taatcattta
    40261 atactttact atatgctgtt atagataaaa agtttacgtc tttaacttca tctatttgct
    40321 tagcactcat attatataat attttttgta aaatatcatc atatttgttg ttttgtaacc
    40381 tttttgtgta ttctccaaca atacaagcag acacatctac ttgagatgaa tatttattta
    40441 acacattttc tacgttacta taaataacct ctttatgttt atatactttg ttatccaata
    40501 tgtcaactac tatgtaactt tctttgttat ttttatttat tccttttata gaaatttctt
    40561 tatcgctacc ttttatagta gcacataatt gtattttatc gccagaccct ttttgattcc
    40621 aatttattcc ttttttatcc aactttagat tgttaattat ttctataaaa atattatgaa
    40681 attctttatt tgataaatta tgatttataa cagaattaat tttaacatta taaaatttaa
    40741 aatttgcttc tgatatttca agagctttac ttaatctatt gtatctagta tttatacttg
    40801 tatctgcata agatattgtc attcctataa tcattaatat aaaaaagctt atagctaatt
    40861 ttactttttt catagtaaaa aatccccctt aaatatgttt ttctgtataa tagaattatt
    40921 aacaaattta agagagatat actttctcaa tatgtatttt tatacatttt tgtatttttc
    40981 atctaataat ctctttcaaa ctcttcaatc tcaattttct ctatccttct cctggacttg
    41041 ccaatttctt atatgtatca aatacatctt gcattaattc ttatatattc tcttctgtaa
    41101 tataaattac ttagtctata cttaaatcta taatctataa tctataatct ataatctata
    41161 atctataatc tattcatatt ttaccaacaa aataaacaga taatactttt ttatcaattt
    41221 ccatagctcc aaatgaacat acctaagtac acttcccaat acacaattta cacactcttc
    41281 ctaataaatc aaaaacacct ttgttttaaa caactctatt gcactttcaa aaactttatt
    41341 tgtgttctcc cacttcaatt aaatttgaat tatcaagact ccagcccatc catccatcac
    41401 tataaaggga ggtattttcc actcctatga catttgcata tgttaatact tcagcagcac
    41461 gccatccact tccacacata aatataagct gtttattcac atctatattt tccttgtccc
    41521 acatttctaa aatctcatat ttatttctca ttgtattatc aatattacga tattcttcta
    41581 gtgaaacact atcactacct gaatgtccat ataaagctcc tggtatacgc ccttttttat
    41641 cataatatgt atatccagat acttttccta tatgttcatc ccaagtacgg ttatctacta
    41701 gtataaactt atttttttct tttagttttt gtcttaattc agatgtagta acaattaatt
    41761 gtgagttaac tggtatatct gctccaaaat tagtacatga atgttttgga ttactaataa
    41821 attctaattc atatcctgct gataaccatg agtttgttcc accattcaaa actcttacat
    41881 cttttactcc tatatatctc aatataacag ctagtctata agatgccata ggtgtactac
    41941 ttgaaactat tacagtatca tcctttgtta atccatagtc taaagcaaac ttagttaagt
    42001 tatcatcatt atcaagcatc catgttggag gaggttctat aatatcagta tttacatgaa
    42061 cacttgtagg tatatgccct tttgtatacg attcttctct ccaactcgct tcaatcattt
    42121 ttatattctt tgaatcttca aatgtctcag gaatttttcc atctaatata tcttttatta
    42181 taaaagcagg aactatcatt tgataatttt tatatctttc cattggaaga ttttcgtcgt
    42241 ctgcccattg tttaatatca tatttatata gatatttata tcctttttta gataaataat
    42301 ctgctacaac caaagcatct tttccatttg catcatacaa tactatattt ttatctaaat
    42361 caattctttt agttttcaat gcttgttcta acacttcatc ttttctatct gagtatacac
    42421 ttaaccaatt agcagaaaaa tctactgctc cttttatatg tccacctctt tttactccat
    42481 ctaacttcca gccattataa gcatcattta agcgagtatc aactataacc caagcgttat
    42541 cttgcacttt ttcttgtaaa acttcagtag atattgcctt aagattaaca aaataatctt
    42601 ctttcttttc ttttttaaaa tcatttgtca taatttatac tcccaattta aaaaaatttt
    42661 tatttgaaat tattttacta aaataaagta attttcttat actctattag cttcattata
    42721 ttcttttata aatagttcct tatattctta ttttaaacta ctcactttat cttatcacaa
    42781 ggttactaat accttgtatt ttaatttttc tcataagcta ttattttttt atctgatatt
    42841 gactatatgt ttattaagta ctcactatat tttatatgaa ctatatttat cgttccctat
    42901 acctggtatg gtctatatat ttatcatttt acagatgatt taatatcaat ctttttataa
    42961 aatttattat agcaaaaaca aaacaaattt aatatttatc taaaaaactc atcctataaa
    43021 atacaaataa caggatgagt tcttttcaat aaatattatc tatttaattt ttaatctgtt
    43081 tttattcaac tgttactgat ttagctagat ttcttggctt atctacatcg catcctctta
    43141 gtacagagac atgataagca agtaattgca ttggtacaac tgctaatatt ggtgataaaa
    43201 tatcatctga atttggtata tagattattt tatcagcagc tttctcaaca tccttattat
    43261 ggctttgagc tattgctact acataagctc ctcttgctct tacttcttcc atattagaaa
    43321 ccattttttc aaataacttt tcttgagtag ctatggcaat tacaggagtt cctttttcta
    43381 ttaaagctat tgttccatgc tttagctctc ctgctgcaaa agcttctgca tgtatatatg
    43441 aaatctcttt taatttcaat gaaccttcca ttgctaagct atagtctata cctcttccta
    43501 aatagaatgc atgttcagaa ctaacaactg ttttagcaac ttcttttata tattcttcat
    43561 tgtctagtat ttcttgtatt tttgatggta tttctttcat cttagaaatc atactatcat
    43621 aaaattctct agttattgtt ccttttttga tagcaaagtc taaagctatc atgtataaag
    43681 aagttatttg agtagtatat gccttagttg atgcaactgc tacttcaggt cctgcccaag
    43741 tatagaatac atcatctgat tctcttgcta tagatgaacc tacaacatta gttattgaaa
    43801 gtattcttgc tcctttagcc tttgaatctc ttaatactgc tagagtatct gctgtctcac
    43861 cagattgact aactaatatt actaatgaat tttcatctac aaatgggtcg ctatatctaa
    43921 attctgatgc tatatcagtt attactggta tatttacaaa tttttctatg gcatacttac
    43981 caagtaatcc agcattatat gctgttccac aagccactat ataaacttta tttatttttt
    44041 ctaagtcttc tttagaaata tttatactgt ctagaattat atttccatta tcatcaagtc
    44101 ttctttctaa agtttctctt actcctgttg gttgctcatg aatttctttt gacataaagt
    44161 agtcatatcc acctttagac gcagcttcta catcccaagt tacatgaaat acttcttttt
    44221 caacaagatt tctattagag tcataaactg ttacattttc gtcttttaga tgaactactt
    44281 ctccattttc taagaaataa acatctctag tatattttaa taatgcaggt atatctgatg
    44341 ctataaagtt ttcaccttca ccaacaccaa ctacaagagg actatctttt cttactgcaa
    44401 ctaattcatt tccatgttct ttacatataa cacctaatgc ataagctcct cttaattttg
    44461 atatagtttt gtatacagca tctaataaat taccctcata gtatttatct actaaatgtg
    44521 ctattacctc tgtatctgtt tgagattcaa acttaacacc ttcagaaatt aattcttctt
    44581 ttatttccat atagttttct attatcccat tatgaacaac tgctattgtt ttggcttgat
    44641 taaaatgagg atgtgagttt acatcagatg gttctccatg agttgcccat ctcgtatgcc
    44701 ctatacctaa atttccatca ataggattct tttctaagtc ttctgcaagt acagaaagtc
    44761 tacctttaaa ctttctaata ttaagttctt tttcattaga actattaact gctactcctg
    44821 ctgagtcata acctctgtac tctagcttag aaagcccttc tactataact tctgctgcct
    44881 ttctacttcc taaatatcca actattccac acattatttg tgtcctccta aaaatattat
    44941 ttatatattt taagagttag gtcatataag tttactttgg cttccctttg tccataggat
    45001 tgctcctatt atttaaagaa gtctttcgat ggtaaacaca ccgcagagca tccgccgaca
    45061 attcgattaa ctctgacctc gtcaacttag ttcctatatt actcaataat tatgtctata
    45121 actaagttct ggcgcttaga aaggtgtcct cagtatttat atctgagaat accctttaaa
    45181 ttacgataat ttttcttgta taagattagc taagtttgtt gctaattctt ttatttgacc
    45241 ttcctcttta ccctctaaca taactctaac taatggctca gttcctgatg gtcttatcaa
    45301 aactctacca tttccgtcta aaatagattc tattctttct atctcagttt ttatttctgg
    45361 atattccata tatttgttct tattttcatt ttttattgtt gcatttacta atacttgtgg
    45421 gtattggctc attattgatg caatttctga taaaggtttc ttttcttgca agattatatt
    45481 tgcaagtatt agagaactaa gcactccatc tccagtagtg ttgtaatcta aaaatatcat
    45541 atgtccagat tgctctccac caagattata tccattttta accatatctt ctaaaacata
    45601 tctgtctcca actgctgtag tagataaatt tattccattt tccttagcag ctattgtcaa
    45661 ccctatatta ctcataactg taactaccaa agtatcttgt gccaatttat tatttttctt
    45721 taaatttaag gcactcaata tcattatatg gtctccatca acaatttgac ccttttcatt
    45781 tactgcaatt aatctatcag catctccatc atatgcaaga cctaagtcag cattatgttc
    45841 caaaacagcc ctttgaagtt gttctggatg tgtagagcca catttatagt ttatattatt
    45901 tccatctggt gaactattta ttgaaattac acttgctcct agttcatcaa acactattgg
    45961 tgccacttta tatgacgcac cattagcaca gtccaaaact acttttaatc ccttaaagtc
    46021 agtgcttatt attgatttta ggtagtctac atagtctctt tgtgcacaat gttcatgtaa
    46081 ttttcttcct acattttcac caattggaag acaatcaatt ttgtctatat cgtctatata
    46141 ttcttctatt ttcaattcaa tttcatcatc taatttatat ccattcttat taaagaattt
    46201 gattccatta tattcaacag gattgtgaga tgcagatata accacaccac aatcagcacc
    46261 atattttcta gttaaatatg ccactgcagg tgttggtatt attccaacag taataacatc
    46321 acatccaaca gacataagtc ctgctattaa agatgcttcc aacatatcac ctgatattct
    46381 agtatcctta cctactacta cttttactct gtggtctcct tgagctaaga caaatccacc
    46441 agctctacca agtttatatg ctaaatcaca tgttagttct gtatttgcta ctcctctaac
    46501 tccatctgtt ccaaaatact ttctcactaa agattctcct ttcataattg aaaagcttgt
    46561 aatttgtatt atatttttat agttcataaa atatcattat aggcttaatt tcagtcataa
    46621 aaatatttta atacttatca gcaaaaaaga caatagaaat aattctattg tctttttttt
    46681 gacatctttt tctttattag ttagttgctt tagaacatgt agcatccata cctttttgta
    46741 aagcttctct gtttaatgga acgaattttt cttttctagg tccgaaaact tttttgaatg
    46801 cttctagtac agattctata tctacagtat cagatacttt taagtaagcg cctaacatta
    46861 tcatgttagc aactttatca tttcctaatt cagctgctaa ttcatttgct ggtatataat
    46921 aaacatctac atctgttcta tcaacttttt cttttattaa agaactattt actattattt
    46981 ttccaccagg aataacatca tctttgaatt tttcaaatgc tggtatattt aataatatag
    47041 ctgcagttgc atcatctgtt ataagtggag aaccaactgg ctcattagat acagttactg
    47101 cacagtttgc tgtacctcca cgcatttctg gtccataaga tggtaaccaa gaaacttctt
    47161 ttccttctag cattcctgca taagtaagta attgtcccat agacataaca ccttggcctc
    47221 cgaagcctgc acatattact ctagctgtag acatttactt cacctcctca acttcaacat
    47281 ttttgaaatt tcctaatgga tagtatggta tcatgttgtc tcttaaccat tgtaaagcat
    47341 cgtttggagc tagtccccag tttgtaggac aagtagataa tacttctact attccaaatc
    47401 ctaatcctgc ttgttgaact tggaatgctt tctttatagc ttttttagct tgtctaacat
    47461 gagctggagt atcaactgct actctttcta caaatacagc tcctgttaat gtagctaaca
    47521 tttcacttac tcttattgga taaccttgag tttcagcatt tcttcctgat tgagcagtag
    47581 tagctctttg tccaactaat gtagttggag ccatttgtcc accagtcata ccataagttg
    47641 tattgtttac aaatatagta gttatttttt ctcctctagc agctgcatga actatttcag
    47701 cagtaccaat tgaagctaag tctccatcac cttgataagt gaatactgta ttttcaggat
    47761 gaactctttt tattcctgta gcagcagctg gcgctctacc atgagcagct tcttgagtgt
    47821 cacagttaaa atatttatat cctaaaactg aacatccaac tggaacaaca cctacagcat
    47881 cacctaatac tcctagttct tctaatactt caccaactaa tctatggatt ataccatgtg
    47941 tacatcctgg acagtagtga gtttgagtat cttgtaatcc ttcagttttc ttaaatacaa
    48001 cagccattat ctagcacctc ctgcaaccaa ttctccggca ataatttttt tagctttttc
    48061 aactatttct gctggtgttg gagtcattcc tcctggtctt ccatggaagt aaactggtaa
    48121 tttaccttct actgccattt taacatcttc caccatttgt cccatactca tttcaacagt
    48181 caatatgttt ctagcattag gaatttggtt gaatgcttcg aatgggaatg gccataatac
    48241 tttaggtctt ataagacctg cttttattcc ttcttctctt aatatatcaa ttgcgctttt
    48301 tactactctt gaagtagttc cataagctgc aaatacaaat tcagcatctt ctgttttgta
    48361 catttcgtat tgtacttcat tcttttccat tgcatcaaat ttttcttgta aatgccaaca
    48421 atggtcttct agtacttctg gctctaagta tagagagttt attacattag gttttctttt
    48481 tcctttagtt ccaacagttg cccaatcttt aggaggcaat tctcttttct tctcaggagc
    48541 tctaaattca actggctcca tcatttgacc tatcatacca tctgcaacta ccataactgg
    48601 tgatctgtaa taatctgcaa cgtcaaaagc ttccattatc atatcaacag cttcttgaac
    48661 tgtagctggt gcgaatactg gagttctgta gtctccattt cctccacctc ttgtagacat
    48721 aaagtagtca gcttgtgaag gttgtatact tccaagtcct ggaccacctc tcattacatt
    48781 aagtacaaca caaggaactt ctgctcctac tgcatatgtt ataccttctt gctttaaagc
    48841 aactcctggt gaagatgaag aagtcataac tcttgctcct gcacctgctc caccataaac
    48901 catgtttatc gcagaaactt ctgattcagc ttgaacgaac gctccaccta ttttaggtaa
    48961 ctctcttgat aaatattctg gtaattcact ttgtggagtt attgggtaac cgaagaaata
    49021 tttgcaacca gcttctatag ctgcttttcc aaatgcttcg ttacctttca taagtatctt
    49081 agccattaaa attccccctt aaatattaat taaattaatc tctttctact gttataacta
    49141 catctggaca catagttgca caacttgcac atcctatgca tttttccatt tcaaaaacag
    49201 ttgctgggtt ataccctttt ttgtttatta catttgagtc taattgtatg atttttactg
    49261 gacatgcttc aacacacaat ccacaacctt tacaacgttc ttgattaaaa gatacttttc
    49321 ctttagccat ttacctaagc ctccttaaac tttctatata tttgagctca aactacatcc
    49381 agtcttctct catatataat tttataggaa aaatttctcc ttctaaatct tttggtaact
    49441 gttcaacaag attttctaaa catgcaacat atcttatcgg tatattacat ttttcagaaa
    49501 cttcttttgc aactttctga cctcttaaaa catcatcaat agtagtaaac ctaataagat
    49561 gagtattatt aattagacct gttaccttta atttagaaga tttttctatt tcttttatat
    49621 attggataac ttcttcagaa gtttgagttt tctccctatt tgcatttatt acaaaaagca
    49681 tatcataatc gccttctttt aacaaatgac taaatcttcc tataacccta gcacctacat
    49741 tatctccacc taaatctata acagaattgt atgaatggtc taccatagga gacattacct
    49801 ctgctgatac tgctggtaaa tctaatgttg gagcattaat tgagctatct attggttgta
    49861 tacctaagga tttcattaac tctttctttt ctctagttct aaagtataca tttaccacat
    49921 ctaaatcggc tatagcaact ttgccagaaa ccatatctct taacttaaca acatagttca
    49981 tcgcaaattc acttttacca cttccatagt gaccagttat gattcttatt cttttatcat
    50041 tagttatcat aaacttgagc ctcttcttca ccagttaaaa ctctaagtcc accttgagct
    50101 aatgcaatca tttcatcttc acctggataa acttttacat ctgctataaa tttaactcta
    50161 tctgcaatca tttctgtaaa catttttgaa tatgcgattc caccagttaa taatattgct
    50221 tttacatctc ccttaagaac tgcagcacta gctcctattt ctttagagat ttgatatgcc
    50281 atagcttcat atgctaattt agctttttca tcaccagctt caattctttc ttcaacttct
    50341 ctagcatcat tagtgtttaa gtatgcaact aatccgccat taccttttat tttcttttta
    50401 atttcatctt gagtatattt tccactaaag cacattttta ctaatgcacc tactggtagt
    50461 ccaccacttc tttctggaga gaaaggtcct tctccatcta atgcgtttgc aacatctact
    50521 attttaccat ttttatgagc tccaacagaa actcctccac ccatatgtgc aactataaga
    50581 tttatatctt catatttctt gtttatttct ctagcatatc ttcttgctat tgccttttga
    50641 tttaaagcat gtactacact tgctctactt atttcaggca taccagaaat tctagcaaca
    50701 tcttctaatt catctacaac aacagggtct actatgtatg aaggaacatt tacttcttca
    50761 cctatttgtt ttgctattat tccacctagg tttgaagcgt gttctcctaa aactcccact
    50821 tttaaatctt caatcatagc agcacttact gaataagtac caccttttat aggtttaaga
    50881 agtcctcctc tacctactac agcatctaat tcagatgttt ttactccacc ttcttttaga
    50941 gcttcttcta ttacttgttt acgaaattca aattggtcag acaccttctc atattttcct
    51001 atttcttctg aagaatgtct taaagttttt tcaaatacta aatcctcatt atcaaataca
    51061 gctattttag ttgatgtcga accaggattt attgttaaga ttttaaatat tttgctcatt
    51121 tgcctatttc ccccttaaaa ttatgccttt gctgccatta aaacacctaa agctattgag
    51181 tttagtttag tttcttcact gtctgctcta gaagttaata ttattggtgc tttagcccca
    51241 actataactc ctgcattttt tgattttgag aagaatacca aagctttata taatatgtta
    51301 ccaccttcaa tatctggggc taataatata tcagctcttc ctgctacagg atgatttata
    51361 cctttatgtt tagctgcttc taaagatact gcattatcaa ttgcaaaagg tccaccaacc
    51421 atacaacctt tgatttctcc tctttcatac atttcttcta gttctttagc ttcaactgta
    51481 tctttcattt ttggatttac tttttctttt gcgcatattg cagcaacttt tggttcactt
    51541 atatctaatg aatgtgctac tgtgcaagca ttttctatga tttgcttttt agtatttgta
    51601 tcaggagcta agttcatagc tgcgtcagtt acgaaaaata atctatcata tccctctaca
    51661 tcaaatactg ctacgtgact taatacattt ccagttctaa gacctacttc tttatttaaa
    51721 actgctttta gtattattga tgtgtctact aagcctttca ttaccatgtc ggcttttcct
    51781 tgtgaaacta attcaacaga ttttagagat gcttctgcta aatcttttat atctatcagt
    51841 tcataatttt cgatatccat gtctatgctt tttgcaattt ctttagtatt ttctatatct
    51901 cctactaaaa tggcatttgc tattttttct tttctagcca tttcaactgc cattaaaact
    51961 tctttatctt ggcaacatgc tactgatata gttttaggtc ctctttcttt tgcaaactta
    52021 attacttctt caaaacttct catgcatttc cacctcatct tgaatttttt gaaatagaac
    52081 tttttatatc tcaattaaat tataactcgt tcagcaacgt tttcctagag ataattgaaa
    52141 tatttaattc aaaatattta tttaaatcta tttatcagtc ttttttcata tatctagcgc
    52201 tttttagatt taacaaatat ctcattgtcc ttattttata atataactaa aataaacaag
    52261 atgcaacaaa aacataaaat taacaaactt aattttatag gtttttttgt cgcatcttgt
    52321 aacaactaaa tattcgaaat atttgttcat tccacttcaa caatattagg ttctatattt
    52381 atttttttaa attgatatgt tgtttcatat tttattttat atttactatc ttcctcagga
    52441 ccttgtgata aatcaatata taatgaaata tcttttttat ttaacttact caaatcctcc
    52501 attgactcta caacaacttt tatatctaca tcagaaatta catttggatt atccatattt
    52561 gttgggatat ttcttaattc aatatctccc gcattatata aaaattctgc actcaattgt
    52621 tttgtatcat ttaatttaat tgttatagag ctatcctcaa gagttatacc ctctggaatt
    52681 tctaagctaa cttctttttc ttcaccatta gccaaatcag ctaagtcaat aggttttgtc
    52741 ttaatataac ttattttgtc tatatcttct ttctttcctt ttattgtaac ttgttcctta
    52801 cttaatttat agtttttcag tacatcatta tctttattat catttagact tactcttatt
    52861 gggacaattt tttccttcaa tagttctatc tttgcagtca caaaggatga acttaatgtt
    52921 actccttcaa ccttagttcc atcactattt aatggtatta attgcaattt tttagaaaaa
    52981 tcatctgtct cattaccaac atctagatta gctcttacac ttttgacttc tttaactaga
    53041 gttcttggac ctgatacatc gacaaattcc ttactaagtt caattttatt tatatttctt
    53101 tttgaatttc cacttacttc tacctttata tctctttttt cttttatgtt tttttcaaga
    53161 tttactacta aaacatcatg cttaaattct agagtaacac cttgaggaat atttgctttt
    53221 aaatatactt ccttctgtcc ttgtattggg tcttcaatgg ttccatatac atgtatatct
    53281 tcttttttaa cactttgtac ctttgataaa ctacctttta tatatacatc tgctgttaat
    53341 ttttcgtctg gatatataac aagattttta tcctttaaat cactcatatt acttatagat
    53401 acaggtatat cttcaaaccc tttagtttct tcagggtcaa ctacagccat aacatacatc
    53461 cataatgcaa ttgcacttaa aaatgatatt atttttattt tggtattatt ttttagtcta
    53521 tttatcattt aaatatacca cctttgaaaa aacttcttgt ttctgtattt gtttttaaat
    53581 tactacgtaa tatattcatc attctttctc tagaaatatc tctatataac ttacctgctt
    53641 tagcaattga tacgccacct gtttcctctg atacaatcaa agttatacag tcagacacct
    53701 cacttacccc tattcctgct ctatgtctag tacccaaatc tttacttaaa tccttacttt
    53761 ctgtcaatgg caaaaaacat gcagctgctt ttaccttaga atctctaatt acaacagctc
    53821 catcgtgtaa tggtgtattt ggtataaata tatttattaa tagttgccta gaaatttcag
    53881 catcaataat agttcctgta tttattatat cacttattct agtttctctc tccattatga
    53941 taagagctcc tattttttgt ctagaaagtg aatataaagc ttctacaatt tcttctatat
    54001 ttctattgag agtttcttca gaagtattta cattctttcc aaataaatta aactttgctc
    54061 ttcctatatg ttcaagtcct gctctaaatt ctggttgaaa aattataaga gcagctataa
    54121 ctccataatc taaagcttta agagatatcc aataaacggt atgcaattta aaggtgttac
    54181 taagttgagt tgctaataaa agaaatatta tacctttaaa cacttgttct gctcttgtat
    54241 cttttataaa cataaatatt ttgtaaaaaa tatacgctac tatagatatg tcaattaaat
    54301 catatatact cattcttaat attatattaa aaaatccatt taaaaaagaa tttatatcta
    54361 acacagtttt atcctcctgt ttgataactt cttaattatt tacactaagt attatactat
    54421 ataaatctaa ctctatacaa actatttaac ttttatgtaa ataattctag ttttatattt
    54481 ggagattgtt aaataatctc taaacacatt aaaaatatca acttaataaa tacatatttc
    54541 ttattaaagt tagtttttac tcaaataact caaaccatca aggcgcgaat acaaaatagt
    54601 actcgcgcct taattcagtt ttatgttcta aaatatctta atatatcttt gtttaatata
    54661 tattaattat tataaattct ataatccact tattaactat aaacaaatac aaaatctaaa
    54721 tattacttat catcaatatt attccattag attcattttt atattaaagt aataacttat
    54781 ttcatatcta gattagataa atttatttta atatcttact ataaactatc attaaaactt
    54841 attatgtata actagatata atctcctaca gtgaaaataa tacacaatac ctaaagttat
    54901 atatccattt cacagtataa taatttattt agtataactt ataaatatta cattaaattg
    54961 taaattcact atagtctcta attttttata atttcttata cttattgttt agacaaaaat
    55021 atgctcatga atctctttat aaataatttc ataagttatt tcaatatata aataccataa
    55081 agtatacaat ataatgtata taactcttac taattagtta tatttattat atttaactga
    55141 tgccttcata aacatcttgt agactattat acattaccct ccgtatgttt acaatattaa
    55201 tgaatatact gtaactctat tagaatatac tgtaattttt acatagttta aagtttataa
    55261 ataaaaagac acaggtaata tacatgtttc ctgtgtcttt ttaaacaata taaaagttta
    55321 ctataattta tagtaaactt ttcttaatga gcgcacaggg attcgaaccc cggacacacg
    55381 ccttagaagg gcgttgctct atccagctga gctatgcacc caagttatta tggagcggga
    55441 aacgagattc gaactcgcga cttcaacctt ggcaaggttg cgctctacca ctgaactatt
    55501 cccgcttatt taatttggcg acctggaagg ggctcgaacc ctcgacctcc agcgtgacag
    55561 gctggcattc taaccaactg aactaccagg ccgcgaatgg tgggaccaac agggctcgaa
    55621 cctgtgaccc cctgcttgta aggcaggtgc tctcccagct gagctatggt cccatttttg
    55681 gagcgggtga aggggatcga accctcacag ctggcttgga aggccagaac tctaccattg
    55741 agctacaccc gcatatttaa ttgaattggt gactcgtagg ggaattgaac ccctgttacc
    55801 gccgtgaaag ggcggtgtct taacctcttg accaacgagc cgttttaatg gttgcggagg
    55861 caggacttga acctacgacc ttcgggttat gagcccgacg agctgccaac tgctccaccc
    55921 cgcgatattg ttttataagt gccgaagacc ggaatcgaac cggtacgaga ggtaagtccc
    55981 gcaggatttt aagtcctgtg cgtctgccag ttccgccact tcggcttttt tggctccaag
    56041 ggtggggctc gaacccacag cctaccggtt aacagccggt tgctccaccg ttgagctacc
    56101 ttggaacatt t
//
LOCUS       DAYASV010000011        55015 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYASV010000011 DAYASV010000000
VERSION     DAYASV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 55015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55015
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.011"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(367..879)
                     /locus_tag="ACGI68_000715"
     CDS             complement(367..879)
                     /locus_tag="ACGI68_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137164.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            1432..2313
                     /locus_tag="ACGI68_000716"
     CDS             1432..2313
                     /locus_tag="ACGI68_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HFH0137165.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKSVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSTKDVEKFINFFSAIPAKDTDDTIKIAEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(2626..3405)
                     /gene="hisJ"
                     /locus_tag="ACGI68_000717"
     CDS             complement(2626..3405)
                     /gene="hisJ"
                     /locus_tag="ACGI68_000717"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HFH0137166.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNKILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLDLVKKYDIPLVLGSDSHCAEEINKYDII"
     gene            complement(3951..4301)
                     /locus_tag="ACGI68_000718"
     CDS             complement(3951..4301)
                     /locus_tag="ACGI68_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HFH0137167.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(4317..4733)
                     /locus_tag="ACGI68_000719"
     CDS             complement(4317..4733)
                     /locus_tag="ACGI68_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HFH0137168.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(4752..4943)
                     /locus_tag="ACGI68_000720"
     CDS             complement(4752..4943)
                     /locus_tag="ACGI68_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HFH0137169.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(5094..5909)
                     /locus_tag="ACGI68_000721"
     CDS             complement(5094..5909)
                     /locus_tag="ACGI68_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFH0137170.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(5956..6894)
                     /locus_tag="ACGI68_000722"
     CDS             complement(5956..6894)
                     /locus_tag="ACGI68_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137171.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSAGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(6891..7763)
                     /locus_tag="ACGI68_000723"
     CDS             complement(6891..7763)
                     /locus_tag="ACGI68_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137172.1"
                     /translation="MNKNLSEKMKIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(7788..9716)
                     /gene="acsV"
                     /locus_tag="ACGI68_000724"
     CDS             complement(7788..9716)
                     /gene="acsV"
                     /locus_tag="ACGI68_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid activation/regeneration protein AcsV"
                     /protein_id="HFH0137173.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKDTLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(9828..10205)
                     /gene="gcvH"
                     /locus_tag="ACGI68_000725"
     CDS             complement(9828..10205)
                     /gene="gcvH"
                     /locus_tag="ACGI68_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFH0137174.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(10292..12418)
                     /gene="acsB"
                     /locus_tag="ACGI68_000726"
     CDS             complement(10292..12418)
                     /gene="acsB"
                     /locus_tag="ACGI68_000726"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HFH0137175.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(12475..13281)
                     /gene="acsE"
                     /locus_tag="ACGI68_000727"
     CDS             complement(12475..13281)
                     /gene="acsE"
                     /locus_tag="ACGI68_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase/acetyl-CoA
                     synthase methytransferase subunit"
                     /protein_id="HFH0137176.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(13331..14698)
                     /gene="acsC"
                     /locus_tag="ACGI68_000728"
     CDS             complement(13331..14698)
                     /gene="acsC"
                     /locus_tag="ACGI68_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HFH0137177.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(14729..15673)
                     /gene="acsD"
                     /locus_tag="ACGI68_000729"
     CDS             complement(14729..15673)
                     /gene="acsD"
                     /locus_tag="ACGI68_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HFH0137178.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(15693..16463)
                     /locus_tag="ACGI68_000730"
     CDS             complement(15693..16463)
                     /locus_tag="ACGI68_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase accessory protein
                     CooC"
                     /protein_id="HFH0137179.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(16519..17904)
                     /gene="lpdA"
                     /locus_tag="ACGI68_000731"
     CDS             complement(16519..17904)
                     /gene="lpdA"
                     /locus_tag="ACGI68_000731"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFH0137180.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTIKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(17948..18829)
                     /locus_tag="ACGI68_000732"
     CDS             complement(17948..18829)
                     /locus_tag="ACGI68_000732"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFH0137181.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(18864..19538)
                     /locus_tag="ACGI68_000733"
     CDS             complement(18864..19538)
                     /locus_tag="ACGI68_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HFH0137182.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(19575..20447)
                     /locus_tag="ACGI68_000734"
     CDS             complement(19575..20447)
                     /locus_tag="ACGI68_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HFH0137183.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(20539..21171)
                     /locus_tag="ACGI68_000735"
     CDS             complement(20539..21171)
                     /locus_tag="ACGI68_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HFH0137184.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(21276..22952)
                     /locus_tag="ACGI68_000736"
     CDS             complement(21276..22952)
                     /locus_tag="ACGI68_000736"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFH0137185.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(23201..23980)
                     /locus_tag="ACGI68_000737"
     CDS             complement(23201..23980)
                     /locus_tag="ACGI68_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0137186.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(24290..26209)
                     /gene="cooS"
                     /locus_tag="ACGI68_000738"
     CDS             complement(24290..26209)
                     /gene="cooS"
                     /locus_tag="ACGI68_000738"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFH0137187.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKSGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(26816..27727)
                     /locus_tag="ACGI68_000739"
     CDS             complement(26816..27727)
                     /locus_tag="ACGI68_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFH0137188.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(27843..28421)
                     /locus_tag="ACGI68_000740"
     CDS             complement(27843..28421)
                     /locus_tag="ACGI68_000740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0137189.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            28647..29570
                     /locus_tag="ACGI68_000741"
     CDS             28647..29570
                     /locus_tag="ACGI68_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0137190.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQDSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(29742..30884)
                     /locus_tag="ACGI68_000742"
     CDS             complement(29742..30884)
                     /locus_tag="ACGI68_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0137191.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELRINK"
     gene            complement(30902..32656)
                     /locus_tag="ACGI68_000743"
     CDS             complement(30902..32656)
                     /locus_tag="ACGI68_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HFH0137192.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            32792..32998
                     /locus_tag="ACGI68_000744"
     CDS             32792..32998
                     /locus_tag="ACGI68_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137193.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(33003..33926)
                     /locus_tag="ACGI68_000745"
     CDS             complement(33003..33926)
                     /locus_tag="ACGI68_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137194.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIIDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(34180..35880)
                     /gene="argS"
                     /locus_tag="ACGI68_000746"
     CDS             complement(34180..35880)
                     /gene="argS"
                     /locus_tag="ACGI68_000746"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFH0137195.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(36342..36755)
                     /locus_tag="ACGI68_000747"
     CDS             complement(36342..36755)
                     /locus_tag="ACGI68_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFH0137196.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(36823..39201)
                     /locus_tag="ACGI68_000748"
     CDS             complement(36823..39201)
                     /locus_tag="ACGI68_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFH0137197.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            39520..40971
                     /locus_tag="ACGI68_000749"
     CDS             39520..40971
                     /locus_tag="ACGI68_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HFH0137198.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(41175..43688)
                     /locus_tag="ACGI68_000750"
     CDS             complement(41175..43688)
                     /locus_tag="ACGI68_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0137199.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(43911..44732)
                     /locus_tag="ACGI68_000751"
     CDS             complement(43911..44732)
                     /locus_tag="ACGI68_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0137200.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(45250..45726)
                     /locus_tag="ACGI68_000752"
     CDS             complement(45250..45726)
                     /locus_tag="ACGI68_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFH0137201.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(45760..46338)
                     /locus_tag="ACGI68_000753"
     CDS             complement(45760..46338)
                     /locus_tag="ACGI68_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0137202.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(46442..49024)
                     /locus_tag="ACGI68_000754"
     CDS             complement(46442..49024)
                     /locus_tag="ACGI68_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFH0137203.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSSISMDLYTSSSLGIPKELMGSNTNNIYTLSNTSKIDTLNKNHSNTA
                     VTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNIDCYLNA
                     FNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVTEYCPMG
                     VVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLDNLYELE
                     EIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKRATDLYKKLDEIGTVAGHYY
                     KGVE"
     gene            complement(49594..50277)
                     /locus_tag="ACGI68_000755"
     CDS             complement(49594..50277)
                     /locus_tag="ACGI68_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFH0137204.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(50602..51192)
                     /gene="zapA"
                     /locus_tag="ACGI68_000756"
     CDS             complement(50602..51192)
                     /gene="zapA"
                     /locus_tag="ACGI68_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFH0137205.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(51217..53610)
                     /gene="pheT"
                     /locus_tag="ACGI68_000757"
     CDS             complement(51217..53610)
                     /gene="pheT"
                     /locus_tag="ACGI68_000757"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFH0137206.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(53627..54646)
                     /gene="pheS"
                     /locus_tag="ACGI68_000758"
     CDS             complement(53627..54646)
                     /gene="pheS"
                     /locus_tag="ACGI68_000758"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFH0137207.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(54696..54930)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT        16862 a        10494 c         6078 g        21581 t
ORIGIN      
        1 acccctattt caaatctttt tccttcaata taatcttttt cttttaattt attttatatt
       61 tttgacgcaa tcgtttttta ttcaatcatt ttatattctt gatacaatca ttcttacact
      121 taattatttt ttgtttttga cacaatcgtt ttttgttgaa tcattttata ttcttgatac
      181 aatcattctt acacttaatt attttttgtt tttgacacaa tcgtttttta ttcagtcatt
      241 taatattttc aacacaatca ttcttacatt ctattattgt ttgtttttga tacaactgtt
      301 tttattcagt catttaatat tctcaacaca attacctttt cattcaatca aagtaatctt
      361 taagcattat ttttcaagct catcaaagta attatatacg ataacctcac ctgtctcttt
      421 atctgtagtc tttataatgc ccttagctcc atttacctct tcttttgtaa attcatcatc
      481 taacacaact ttgaatttat cttcctttcc ataaccttgt tgaaattcat ctctcaaaat
      541 tgcatttttt tctgcttttg gcttcaactc aaattcaatt ccagatttca tttcatcaaa
      601 ataatttaat actacaacct cattattcat tttattagtt gtctttataa tccctttagc
      661 tccatttact tcttcttttg taaattcatc atccaatgtt atattaaatt tgtcttcttt
      721 tccatattcc tcttgaaaat ccctcttcat ttcattgtat ttagtttcaa actctttact
      781 cttgtttaat tccatagctg atacatttgt caatgatgct ccaattgcca ttactgttaa
      841 tgccattact ataccacttt taaatttttt tactttcata tcttacctcc taaattaaat
      901 gttctcaatg aaattatata aagaaaatcc taagttttac agtttcattc ttgtaactaa
      961 aattactatt atgtaaccaa actttttgct tcaactattc cttaaaagtt gcttttttga
     1021 ggaatagttg aagttaaata attagatttt atctatattt atagatttaa aatcaaattt
     1081 atccatataa ataaatactt atttttgcaa aataaaaatt tttaattact ccaatccttc
     1141 ttatttactt aaaatcctat gataagaaat ttaaaagtct ttaccttctt taacaataat
     1201 ttatttactt ttaacataca aaatatagag agtatatcaa aactatttta ctgaatattc
     1261 taaaaataag taccccctat tagtatatat atttacatat taaaaaaaag ttttaaataa
     1321 agttatattt tttacaacag ttttgtaatt aaaaacatat tgaaggtaaa tttaaaggta
     1381 ctatatacta taaatatgtc gacacaataa atttaaaaag gagaactaaa tatgaaaaaa
     1441 agattaataa taatgatatt gtctgttgtc ttagtactat caagtatact tacgatattt
     1501 gcatattcaa atattaaata taataataac aaattgatat attctaatat gatagataaa
     1561 aaaactcaaa atagtgtaaa ggaaatccta aaagaaaata aaattaatga aaaagacata
     1621 gatacattta ttaaatcagt caacaattat aataaattgc aagttaaaat acttcaaaat
     1681 aatataaata tatctaaatc tggatattct tcaatcaatg caaaacaagt tccatataac
     1741 ctagaaaaat tgcaagataa ttgggtgaaa aaattccctg attatatgga tgtaaattgt
     1801 agaataactg cttttagact ttttaaagat tttataaatt caaataagaa atttactgga
     1861 gactctattg atttgaatgt cgacttggac actataatga ataataaaga tgctaaattt
     1921 agcaccaaag atgtagaaaa atttatcaac ttcttttctg caataccagc taaagataca
     1981 gatgatacaa tcaaaattgc agaacaaata aaaaatgaat ggaaaaaaag aaaaatatct
     2041 tttaaaaata ataaaaatat atctattata aatggatttc ttcgttatcc agaaactaaa
     2101 aatgtattta taggtcatac aggcatatgt attaaaacta aaaatggcat tctattttta
     2161 gaaaaatacg gtgttacatc accttatcaa gttactaaat ttaaaaataa agaagatgta
     2221 aaaaattata tgtttaatag attgaagatg tcagaaggtg aaattgaatt acctgaccct
     2281 ataataatgg aaaacgataa acttatgaaa taataacttt aaacgcaaaa tagtatttat
     2341 aattactaag tcaatataat agagaaggtc tatagatttg tgtaaataca agcttatcta
     2401 caatcttata taagaatgtt tatataaata agatagatta attctaagtc ctattattct
     2461 attgaatttt attattattt tatttagatt ctattgttat tttacttaga acttattatt
     2521 attttgttta agttctatta ctattttcaa tcatataaat ttacaaaaga ctgtacataa
     2581 atttatttta tacaaactta tctacagtct tttgtgtgaa tatatctaaa taatatcgta
     2641 tttatttatt tcctctgcac agtgagaatc agaacctaaa actaatggaa tatcgtattt
     2701 ttttaccaag tctaataagt atccatctat ataaggctct ttgcagtact cttttctatt
     2761 tccagaaaca ttataatcaa gttcataacc tttttcttta attaacttta ttaactcttc
     2821 aagaatttta tttcctttat aatcgtatgg aaatacctga ttaaactttc taactaaatt
     2881 taaatgacct attctttttg gtttatgtat acctaaatct gaatttacag cttttatcaa
     2941 tgtttcataa tacttattat aaacagcttc aatacttcct aatttgtcga ttaaattttg
     3001 aaactcctct acactgtaat ctacacaata ataatcatca tcaatcttaa ttatatgtac
     3061 tgataaaaga gaatcctcta attcctttcc atatttattt aaattctcct ttatcccttc
     3121 ttcgtatcct tctataaaat ccacttccga cccaacattt atcttgattt taccctcata
     3181 cttttctttc aacttcttga catcattaaa atactttggt aaatcttcaa tactcataca
     3241 actatcattt tcaggagcag ggtctctaaa tccatttgca tacggaaaat gttctgtaaa
     3301 tgtcatttct gttattccaa catccattgc tctttgtaca tatttttcaa aatcatcctt
     3361 acttccatga ggacaataag gtgaatgtat atgaccatct ttcataaata attcactcct
     3421 tttttaaaga aaaaatctaa tataaatgca taaatctttt aacttcaaaa taatattttt
     3481 aatcatgcta atttttatta tatctaatat tagagtaatt ttaaactgcc ttttaagtat
     3541 cttatcatta gtactatttt caacttaaca ttaatattat tttttaactt aacattaata
     3601 ctatctttaa atagtaatac tcaatatata attacttaac ataaaataac tcaaattgct
     3661 tgatgaggct tttgcagtat ttaaagaata tataattatt taacattaaa taatccaatc
     3721 ttgtaataaa atttactcat taaagtatat tacagaaaat acctcaaaat aaacttttta
     3781 gttcatgata tactcttttt tgcatgaaat tagatatatt tttatcattt caacaatgaa
     3841 agggaggctt atatccatct ttagacaacc ccttagataa aatttttaat aattaaacat
     3901 cacaaaatat aaaaatcgct tatatcaaac gtataagcga ttaattatat ttacatatgt
     3961 tttttagcct ctaacaacat tttattagct tcttccatat actctatagc cttttcaata
     4021 ctctttgctg tttcttcttt tccaatagct ctagcctttt caacccactc tctaaatcct
     4081 tcttcatgag tttcattgtg atttatccaa tgtactaaaa gtatttttaa agttttttca
     4141 tccttagact ctgatgattc ctcatgacta tgttcgtggt cgtggtcgtg agcatggtca
     4201 tgtggatgtt catgacatat atctccatga gtatgttcgt ggcagtggtc atggtcatga
     4261 tgtccatgat gatatcttac aatctcttta tcaaaaatca ttagttattc cccctgttat
     4321 tttatattaa actctttttt tataaggttt acagaatctt ttacaagtag tgctaatggt
     4381 ttaccttcta atccaactaa ctccacattt tctggcataa taggtaaaag tatcttaagt
     4441 gcctcactgt ctgcaatagc ttcacttata ttacaagtaa cttctcccat catagaatta
     4501 ggtattatta cagaaatagg tgcaactatt atatttgctt ttttagctga tactactata
     4561 gcattttccc ctgtagctcc tttattagca tgagctttca tcatagaact agttgctata
     4621 gaattagtcc ctagtgcata aatttctaca tatgtaggta actcttctct caaagaagaa
     4681 actatctggg ctccaatacc tccacccatt ccatctatca ctgctattat catatgaaat
     4741 tcccctatta attaattttc ttgtattatt atcttatggt ccatcaatct tatttcttta
     4801 attatcccat ctattatctt ttgctctcct aataaatccg ttaaataaat ctttccatca
     4861 caagggtcta tattaaccac attttccata accttttcta actcattatt acttttacaa
     4921 atgaaagctg atgattcaca catataaatc tcctcctaat atgattttta ttaaaaatca
     4981 aaaaaagatt cccttaggta tttcaataca ccaaagaaac cttcatgatt tcagtttttg
     5041 tatattatta taccttaagt atatcacttt aatttatgta aagtcaatta aagttaacgc
     5101 tttttattat actcttcatt cttgtatttt tcatatagtt ctttaactaa ttgcttctca
     5161 taatcagtca aatcgccctc cacaaactca acattaaatt gttcagctaa tccctttact
     5221 atgtttttct gtagaatatc tatgtctatt tttctgccaa tttcattttc tattccactt
     5281 gcttttttag ctgtaaattt catagctctt tctttcaatt ctggtgtctt agtttttatt
     5341 aatttaaaca atttttctac atcaaaatcc agtattattg aaccatgttg taataaaaca
     5401 ccatcttttc tactttgagc acttccaatt accttcttat tattaatagt aacttcatat
     5461 gatgcatgtg cattaaaaca tgcagctgat aaattttccc tacttattct ctcgcctcta
     5521 tttaaattat ctgtttctat accacttaga ttcaaccctt taactagacc ttcacttata
     5581 tatctataag ataggttgat gctcttatcg attaaaggat tatcttctcc tataattata
     5641 ctataggtta gctcattatc atgtaataca gctcttccac ctgtaattct tctagtatag
     5701 tctatattca tacatctaca cttatctaaa tctatctcat cttccaattt ctgaaaatat
     5761 cctatactca aacaagctgg ttcccatgta taaaatctaa gagttggctt attatgtcct
     5821 ttcttatatg cagtaaatat agcttcgtca attgccatat tcattgctcc ttcatatgat
     5881 ttattatgta taactctcca ctgattcatg attattacgc ctccattttt agtataaata
     5941 tatcctagaa atcctctata gatttatttc tcttattgct aaatctatct cactttgatt
     6001 taaaggtctt gaatagttgt atatagtact tcctggtctt tcattatagt atggtctatt
     6061 acaatcctta caacctctta tttcaaaagg atagcctttg tttatgtctt ttaaaatatc
     6121 attgtcaagc tttatactat ttaagtatcc atttttaaac ttaaaatact cttttggata
     6181 acctttattt atcatataag acattaactg tacccttcta taactttcta tgcttggttg
     6241 atttatcttt tccattttag ttcctctaac tggagtaaat gcaaataaac taatcgttac
     6301 atcattttcc tttaaataag tatataaatt gtatatatct tcatgacttt cacccatacc
     6361 aactattata tgtgtactta ttttattggg atatgatttt gacatttcag ttataaattt
     6421 taacttttca tcatagttat ttccttttat tttttcatat agttctttat ttgctgcatc
     6481 tattgctatt cctattttat caactcctgc tgaaaaaaac ttctttatct gttcatcatt
     6541 ttctaatttt gcagatagtg aaattggcat gtctatctta ccacttatat agtttataaa
     6601 gtctaaaaca gagttatgtg cttcttcact tgccattgat tgaatacata ttctctttat
     6661 attttttcct ttataagctt ttaaagcatc taatatttct tcttttgaaa actctggcca
     6721 aattactctt gataatttat cttttctagt actactttct attgattgag aacaaaaact
     6781 gcatttattt atgcattttt ctccaatcat tatatatgct gtggtaggag gtatatcgct
     6841 ttttttgttt aatatcccaa gttctattgc tgtcccaact gatagtctaa tcataaaacg
     6901 caacactcct tacactcatt tattgtcata ttcatttcta tagccttatt ctttgcagac
     6961 cttgatggta atactattct attgactcca cacattatag ataattggtc tagctctttt
     7021 ctatacactc ctcttggtct catacaacct agattaattt cagtatctgg taaattaatt
     7081 ctagcttcac ataaaatatc tgctacccct tctaactcag gaggttctac attttcatac
     7141 tcagtccctt ttgttggaat aaggactata aatgtaagct tattaggagg atttttttgt
     7201 aggtattcta taatttcata ttcacccttt atttgtcctc cttttaaacc aatacatata
     7261 tgtggtgcta cttctgtatg ttcttgtatt gtattataga cttcaatata atcttcttta
     7321 ctctttttca tcttataaac ttctcttatt gtctcatcat caaacactaa atcaaaagaa
     7381 actatatcta aatacttaca caactctaca atgctatctt tatccataag acctaaatga
     7441 gcattcaatc tatatccttg ctcttttaag tttttaatgg tattaatatg agtatttata
     7501 gggacatctc cctcataact acaaccgcca ctcaatagaa agctagttat attccttgat
     7561 tgtacttttt cctcatattc ttctattggc accatattgt ttaagtagtg accgttgcaa
     7621 tgggcacatt tcaaactaca ttgattgctt gtagtactaa gagctagtgt ctggttagga
     7681 taagcaaact ctattttatt tccaaaattc tttaatttaa tatcaaatgc tattttcatt
     7741 ttttcagata gattcttatt catagtttat tccttcttaa gtacatttta ctcttctagt
     7801 aatttctttg ctgttgggaa ctgctctata ttcgtatgtg gtaagaaaca tgcagaaata
     7861 aattcatcca tataacttgg atatacacta agttcaacat aagttatttg attacctatt
     7921 tcttcaagtt tattttttgc atctttactt ataagcgcta agtaagaacc aacaagtgaa
     7981 ctattcccta tataagtaaa tttttctcta tcaatatcag gaagtaaccc tataattatt
     8041 gagttttcta tgtctaggtt gtttcctata ccacctgcta tataaaccct atctaatata
     8101 ctaaaatcca tacctaaact atctacaaga gtgtatgcgc ctgaatatat agctccttta
     8161 gctctgatga aattatctat atcaacttca ttgactacta tatcattttc taaatcaaat
     8221 tcttctttaa atgctaatac atactctcct atctcatgct cattaaatct aactctctta
     8281 ttatctaaat ccctgtatat tttacctctt ctatctatta cacctttagt aatcatttga
     8341 cagattaaat caattatacc agaaccacat ataccaactg gtgcacattc atcaataatc
     8401 tttaatgttg gctctaaagt atctttatca ataattactt tctctattgc tcctgctgat
     8461 gctctcattc cacagcttat ccctccacct tcaaaagcag gtcctgctga acatgcacag
     8521 ctcatcatat aatctttatt tccaaataca atctctccat tagttcctaa atctataaat
     8581 aaaacatttt cttcacttga ccatatccct gctgataata cacctgctgt tatatctcct
     8641 cctacataac ttgcaacaga tggagctaag tacacatatg cactgtcatt tacaaaaaga
     8701 ccaacatttt cacccataag ttttggagat tttaaaaatg gtggtatgta aggttcttgt
     8761 cttaagaagt ctgtatacac acccaagaat aaacttgtca tagttgtatt tccggcagcc
     8821 actaatgtaa ctacatcttc cttatctatt ccatttcttt catatattgt ttttaatagt
     8881 ggatttatag tttcctcaac tatagcttta tgaagtgtct ctaatccatt tttcttagtt
     8941 gaatatataa ttctatttat aacatctgct ccatatttta tctgtgcatt tccagaagat
     9001 gctttatcta ctacctcttt tgagtatagg tctactaaac aaacaactac tgaagttgta
     9061 ccaatatcta ttgcaactcc gtataaggaa ttttctttat ttccttgttc tatatttatg
     9121 attgtaagtt tcttttgttt ttgaacatat gttattgtaa ccttaaaatc actctttcta
     9181 aatacagttg gcatttttct aagtatatcc aatctaaaat ctatttcatt atatcctagg
     9241 ttatttctaa catatctttc caatctatct acatcactta tattatcatc taaatttggt
     9301 tcttccattt ctatgtattt cttttttata tttgttttaa attgaaggtt atgttcttca
     9361 attatttttt tagctctttc aaaaatttcc ctatcctctt ttttattact accttctatc
     9421 ttcataccat gcatagaaga tgatacttta gaaggaactt caatttctat atctgatatt
     9481 acctttgtac aacaagctag tatataacct tgctcccact catcatctgt tatatgtctt
     9541 gttttttctg tatcaacttt tccattcaat aattttactt tacacttccc acaagacacg
     9601 tttccattac aaggagcatc tatgaatata tctgcatttc ttgcaacctc tagtaatata
     9661 tccccctcat tacaataaac ctctttattg tttggtgtaa aacttacttt tatcatgtta
     9721 tccctcccaa tttgttctat atatgataaa aggaaagtat agctaaattt gcgtgcactc
     9781 aaattttact atactttccc caattataat aatatcttaa tctttaatta ttctaatcca
     9841 gcctcgtatt ctttatcact taataacgta tcaagttcag ccacatcagc tagttttact
     9901 tttaatatcc agttttcata tgggtcttca tttatacatt ctggctcatc ttctaatttt
     9961 tcattaactt ccaatacctc accattaaca ggagatatta aatcagaagc tactttagaa
    10021 gactcaacta ctccaaaatc tactccctta gttacagtat cttctacttc tggtgtttca
    10081 acaaacaata tttctcctaa ttgatcttga gcatagtctg ttatacctat atatacaaca
    10141 tctccatcaa ttacttttac ccactcatga tctttagagt attttaattc tggtaataat
    10201 ttcatttcga tagaccccca atattatatt attgattaaa ttacttaaat catattaata
    10261 ttatatcata ttattttaaa ttttccatca attacattac actttccata gctaatgctg
    10321 gatgaccttt ttcttctaag aaagctaata ctccttcaga atcgtcagct atagtttcat
    10381 cacaaaccat atcacagaag ttttctattc cagtcatttc tttaactgta gcatttaatt
    10441 tatcagctac ataatctttt aattcttttg gcatccaaac tattctttct ggtccacctt
    10501 ctgcataagc gaatttttta gaagatataa aatgtcttcc gtgtcccata aatcctggag
    10561 tttgaactcc tccacctgtc atagaagcaa gttctccgaa agtcatacca actggagtaa
    10621 cacttgcaaa ttctctgtta actacaacaa atccatttgc ttctggcatt ataccacaga
    10681 tacattcgaa gcatccacaa gaagtcattg ggtcttctag tatagagtat aatgtaacag
    10741 attcaactgc accttgagat atttggttta cagtttcatt tactgaattc catacacctg
    10801 ttctatcatc taagcactcg cctttttcta ttggttgaca aggacctgtt gggtctaatt
    10861 ctttagtagc cttagcatct aaccagctaa ctgctccaca aagaccaagt ctttcaggag
    10921 taacaacaca aacgtgagct ggtgcaaatg attgacataa gttacaagaa tagaaagtat
    10981 caacactttc atcaactaat gaagctaatc tttcatctct agcattgtac ttagctattg
    11041 cttctccttt taattcagaa actttttcag catctgtaat tatagttatt tcacacttat
    11101 caactactga ttcaaattca tctaacatct tagcatataa aacttctcct atatgctcaa
    11161 gtctaaatcc tttatcaaat gcatctttag agattcttac ccaagccata tctctttgtc
    11221 caacgtgcat tacaccttct atatagttta agaagtagtg gaaacgtctt tcaagaactg
    11281 gctcgaaatc ttcttgcata tttttaccag ctattttaac tattacagca agtggtaatc
    11341 ttaatacacc atctgcatca acttcatcta tatttggccc tattatttct atcttatggt
    11401 cttcaacttc tgttgcttct ttcttaacaa caagctccca agcttcagtt ctgtttccac
    11461 caaactcagc aaacatatcg ctctttctaa ttctctcacc ttcaaatgct gctgcaaatg
    11521 aaactgggat aggtatttca gtaactttta tttttattcc tctagcttct aatgaagtag
    11581 ctactatctt atcgtaatct ttttgagtta ataggttcat tggaacttca agaactgttt
    11641 ggtcagttat aactgggaat cctaaagcta ttgctccagc acctgcagat acaactaatt
    11701 cacttaatgg tccaaatgca ttaacaaatg caggaactct attagctgtg tactctaata
    11761 atccgtttaa atcaccagga gttaatccac cgaatattaa tgctgctctt actgcaacag
    11821 atacaacgtg tataacagaa gttacatcat atcctagagg tataactctt agctcaagac
    11881 ccatttttac tccagcttct atagcttggt ctataacttt accaactaag aaagttagta
    11941 aacctttaga ttggtaatct tttataactt ttgctgctga ttctgcatct ggacactcac
    12001 caagaacaac tgcaacacca ggtatatctc ctgtaactaa tggtacacca agtgatctga
    12061 ttatagggtc agttatatga cctgcacatg gctcactata tggagcatcc atagttgaat
    12121 attttaatgc ttcaattact tctgctgcta aagctgtagc taaaccagca ttaaaagcat
    12181 gttctagtaa atgagttcta tttattaaag attttactac ttctaaagca ccttctaagt
    12241 cccctaaagt gttcatcttt tgtcctgttg cagcatatat acaaggtaat gaatatgctg
    12301 tatcagggaa agcaacttta tgctcttttc cattcttttc tatagcttca gctaacatag
    12361 cttgagccgc acctaaagct tgttctgacc ctgtaaagat tatattatat agattcattc
    12421 tatttccccc tttaatattt acacctattt tttattaact ataccctaaa aaccctagaa
    12481 agcaaatcca ccttcattta actctaagaa agagtctgca tataaagatg ccccgttgat
    12541 tatgtcacaa gattttacag ttttcattaa ttctgggtca catggattca ttatagctga
    12601 actaaatcca ttagccattg ccattgctaa gaatgcacta tctaaaacag gtctaacatg
    12661 cttaggacat ccattagata cgttagataa tcctcctgta gttaaaagtc ccatctcagt
    12721 catcattttt atagcttcta aaacttctac ttgtttttct tgcatacctt ttataactaa
    12781 gcataatggg tcaaatagta tatcagttgg ctccattcct aacattaagc ctttttctaa
    12841 tatttcttgg cagtaagcca tacgctcatc attatctctt gggatacctt cttttgcaca
    12901 taaacctata accattgctt cgtattcagc tgctatatct attaaatcaa atcttgagcc
    12961 agcatcagca gagtttatta ttggttttgc attagttcta tcataaactt taagtccagc
    13021 ttcgatagct tttttattag ctgtatctaa tgctatagga acattgttga actcagattg
    13081 aagtaatttg attccccatg tcattatttc ttcgccatct ctttcggcag gtcctatatt
    13141 aaaatctata tagtgtgctc ctgcttctaa ttgctctttt gctcttttta agattggagc
    13201 tgggtctctt tctgctaatg cttttcttat tgaaggtgat atacaatgta ttctctcacc
    13261 tataatcata aatttttcca tttataatgt ccccctttaa atttaatatt aatagttagt
    13321 taatatttta ttagtttgtt tggcatgctt tttcttggta tccttttaag aacttaggta
    13381 attccataga ttcctctggt cctataacta cattccatcc tggtaagtta tcttctatat
    13441 cacctttaag tactgcaact tttcctggaa tgataaggtc tttacaatta gttacttctt
    13501 ctaccttact ttctttaatg aaagcagaga ttgagtttcc accaaattta ccagcagccc
    13561 aagatgtaag aactgaatat ccacctgcat ctggtattac taaccatact ggaacttttg
    13621 atctttctat atcaccagca actatgaagt aagttaatgc aaagtcaaca gtaactaata
    13681 ctggagaatt ttcatctgga ttgttgattg gataaatctt tggttcaact ctcattggtc
    13741 tttgtgggtc agtgtatatg ttttgtctta gtgcaaataa tggtaatgcc ttagcataac
    13801 taatatcatc tattactatt atagatccgt attttatagt gaatattgat gataaagcaa
    13861 cttccatcat agggtttgaa ttagataatc tatttgcaaa tactattgaa ggatatccaa
    13921 atgttctatc ttgctctttt aaagctgttc ttctaacttg tatagcatta gtgtaagtat
    13981 cttttatgtt ttctcctgtt acatctaata ctaattcttt atatccagcg gcttgaatta
    14041 attcaactgt ttcatataat tcttctaagc taccagcttt tacacctaat ggtaaacttg
    14101 ctcctttaac tacttctatc atatctttgt agttctcttt agttgctcca tataccattg
    14161 gtctagcatc ttttaatact tcaacagctt ctttaactac ttgtgcatca tcacaagcta
    14221 aaatataagc tacttctaat ccactgcctt ttattttatc tattaatttt aaatacgcat
    14281 ctttatctcc acaatactct aaaacagcca tttcaacttt catttgttca ccaattctta
    14341 catagtcaac ttttttcatg ttagctattt tagcatctac tgcttcatca ctcatacaag
    14401 tacagaatga aactgcatat ctatttctac ttactaatgt tttttcatgt ctaaataata
    14461 ctgtttctcc acctagttca tactcagatg caccagcacc aacttttaaa gctttcatta
    14521 aaggtgcagt agcctctgat aattttgcta tagcgtcatc agacatatgt gggcatttac
    14581 ctacttctac agctcctgaa gcaaccttca tagagaaagc catacaagtt gggaatccac
    14641 aatccttaca gtttttcttt ggtgttaatt taaatatatc taaagctttt aatgccatta
    14701 ttttttcccc ctaaccttaa caatttaatt aagctaatgc gttaaccaat tcttttatag
    14761 tttctattga ttttggatga cgaagtataa ctgcgtttga accacctact aaaacacttg
    14821 ctgcagttga aacttccatt gctatagttc tttcttctgg acaaccccaa tctggctcat
    14881 cttctattgg agctatagct tctttaacat gacctacttc aaaagctact ggtgttatga
    14941 taggcatttg taatgtctta tcattttgac caaatgctgc aagtctaatt ctatccatag
    15001 tagatgcaac gtactcatat ccataaccaa ctgctgaaca tccaacattc ataactatat
    15061 tttcaccttt aacgcctagt tgagttaaca atacgtttaa ttgtttagct aggtttatat
    15121 caacagaaga ttcagctcct actttatgtg cataagccat accagctgat gctcctactc
    15181 ctttgtagtt atcttctact gctgacataa ataagcagtt atgtccatcc aatgtttgag
    15241 ctaatttttc aaataattta ccatcttttt catgatttcc tgaaccagct acaactaaag
    15301 gtaattttat tgcttcaatt actgccttag ctacatcagc acattcatca actgacttat
    15361 ctaatccatt tgggtcagaa ccatcaaatt ttaaacaaat aaaatctgct tgtgtattag
    15421 cttcaacata tttagcccac tctactggac aattagctac atctttgtat aattccttat
    15481 atgaatcagt ccagctttca ggataaacgt ctgatatttg tatacctatt tttggagaat
    15541 tacctacttc tccatcaaaa ctataaaaag gtaatacatt ttccccacct aatttaattg
    15601 ctttttcccc tattcctact tcaacttctg atatttttcc agaatatttt tgagtagaca
    15661 ttttaaatgc catatttttt tcccccttaa gcctatttta attctaattt agcaaaaatt
    15721 tctttcatag ccactttaga ttttgaatct tcaggtaaat taactaaagg aatacctgta
    15781 gaatcatatt catatatcaa ttcatccata ggtacaacac cgattagctc taggttatgc
    15841 ttttcaattt cttctttaac accatcattc ataaccccat ttggaacttt atttacaatc
    15901 aaaagtattc ttccaacact taactttagt tcctcagcta aatctcttat tctagcaaca
    15961 gcttgtatgc tacgtcttga gcaatcacta accaataaaa gtgtatcaac atgtctagtg
    16021 acttttctac ttagatgttc cataccagct tcgttgtcca tgactaagta tttgtaatga
    16081 ccagatattt tgtttacttg ttctctaagt atcccattta caaaacagta acatccttca
    16141 ccttctgacc tacccattac taataagtca tatccttcgc cttcttcgat tatagaattt
    16201 aatctaaatt gaagatattg tgcctttgtc ataccaccag ggaatgcatt gcctaacttt
    16261 tctctttgat ttacttcttc tcttatttct cctattgttg cttcaacttc gatacctaac
    16321 acttcattta tgttagcatt ggcatcagca tctacaacta atactggtcc ctttttgtcc
    16381 ttaaccaaat aatcaatcaa aagccctgtg agacttgttt taccagttcc accttttcct
    16441 gcaacagcta tattgtatcc cattttaaga cttccccctt aaatctagcc acagagcctt
    16501 atgtactctg tctgtctatt ataatttagg tacagaatga acacattctc catgtacatc
    16561 atgaagtgct tccattagcc cttcagataa acttggatgt ggatgtattg catcccctac
    16621 ttgttctact gttaatccta gatgaacagc aagtgacaac tctgtcaata aatctgtagc
    16681 atgaggtcct actactgctg cacctattat cttatcagtt tctttgtcag ctataacttt
    16741 tacaaatcct tggaaatgtc ctatagcttg tgcttttcca agacctctaa aatcaaattg
    16801 acctacattg tattctacac cttcagcttc aagttgcttt tcagtcttac caacaccagc
    16861 aacttcaggt tcagtataaa cacatcttgg aattgctcta taatctacta ccttagtctt
    16921 acctaaagca ttttcaactg ctacgatacc ttctttagaa gcaacatgtg ctaggaaagg
    16981 agtatctatt atatcgccta ttgcgtatat accttctaca ttagtttcaa gatgttcatt
    17041 aactactact tttcctcttt ccatttcaat tcctatatct tctactccag aattgtcaag
    17101 gttaggtctt ctaccaacac atactaatgc atattgtgct tctataacct ttccattaga
    17161 aagagttgca acagccttac catcaactac ttcacaagtt tgtactccaa tacctgtaat
    17221 aaccttaatt ttatctttct taaattgtct aagtagttgt ttagctacat ctttgtcttc
    17281 atttaatagt atttggtcaa ccatctcaac tatagttact tctgttccta aggctctaaa
    17341 gaactgccct atctcacatc ctataactcc accacctact attaacatag actcaggtat
    17401 ctcttcaagt ccaagaactt catcactagt tattacaact tttccatcat atgggaacat
    17461 tcttggtaca actggaacag aaccatttgc tagtattatc ttatctgctt ttatagtttc
    17521 tactgttcca tcatctttag ttacttctat agtgttttta tcaattaatt ttccaaaacc
    17581 attaactaag tttactcctc ttttttcaaa taagaactct ataccactta ttaattggtt
    17641 tacaacttta ttttttcttt ccattatggc agtgaagttt ggcttaattg taccatctat
    17701 ttctatacca aaatcctttg cttcttttac agtattcaat actcctgaag aagcaagaag
    17761 tgctttagtt ggtatacatc ctgcatttaa gcaagttcct ccaactcttc ttttttcaac
    17821 aactgttaca tctgcaccaa gcatagaagc ttttatagct gctacatatc ctcctggtcc
    17881 accacctact actactatct tcatctttaa ttcccccaaa cctaatataa tctacaatct
    17941 tgcaatacta taaacctgcg gcatccagta tagcaggtat attctcttct gccccaattg
    18001 ccattatatg aacaccatca cataaatctt cttcttttaa ttgtttgata agttcccctg
    18061 ccatttttat tccttcactt acccagtttt cttttccagc tgctcttaat ctttcgattt
    18121 gttcatctgg aacaaatatt cctggtacat ttgcagtcat aaatttagcc attcctggtg
    18181 attttaaagg tactattcct gctaatacct tgcaatccat atcccttgtt aattctctaa
    18241 actttctcat tgtatttatg tcataaagtg cttgtgtttg gaaaaattta gcccctgctt
    18301 ttattttctt tttcattttt aataattgaa cttctattgg agaatattct ggagttactg
    18361 atgctcctag ataaaaatct ggagaacctt ttaacttatt accagccatg tccgtacctg
    18421 ccattaaagt ctcagcagtt tgaagtatcc ccacagaatc taaatcaaac actcccttag
    18481 cttgtggatg gtctcctacg ctagtatggt ctcctgttag tgccaacaga ttgtttatcc
    18541 caaatacacc tgctgacaac atttctcctt gtatagctat tctatttcta tctctccctg
    18601 ttatttgaat aactggctct aatccagcat cttttaataa tttacatgta gcaagagaag
    18661 ttgctctcat aactgccgat tgaaagtctg ttacatttgc tgcatgaact cttccaacta
    18721 acggctttgc acattctatt aaatgtgaaa ggtcagtccc ttttggagga gccatttcag
    18781 tagtaactgc aaattttcca ctttctaatg tttctcttaa taagctcatc tctaatcccc
    18841 cttttaacct tctaaaaaaa cttttatgaa tttgctgctg cttctttctt tttctttgca
    18901 cttaaacttc ttggattatt ttgcttagaa tagtcttttg gtggtcttat ttctgctaag
    18961 ttatctaatt gtcctatagc ttctagtctt tcatatatct ttatccaagc acaatcattc
    19021 tctgagttta cttcacactt accattttta gctccaccac aagccccatt cataagacct
    19081 ttagcacaca tagttactgg acatatccca cctgtccaac caagttcaca gtctccacaa
    19141 gccttacagg cttcttcata ttctccaact ctttgtactt cccctataaa caaagtattg
    19201 ttggctggat aaactggctt atcttttaaa ttcttaacta ttgtctgtgt accatctcca
    19261 catgctaaag ataaaactgc atcagcttcc ttagtctctt cttttagagc ctttaaatcc
    19321 tttttagact ttaaaaggtt acaagcaggg tcaagcatta catatcctaa gatttttttg
    19381 ccttcacctt caagagtttc cttcattttt aaaacttctt cttccccacc acttttacaa
    19441 gttgctgcac attgattaca accaactaaa accagcttgt caaaatcttt taagtaccct
    19501 agcacttctt ctaatggttt attttcagaa attatcatat ctacttcccc cctaaacctt
    19561 ccacatatat atatctattt attgttttgt aatttgcaag ccttaactac atgttttgca
    19621 agtatagatg ttgtaactga accaaccccc gctggaactg gagttatcat tgatactttg
    19681 tctaaaactg catttgtatc aacatctcca cataaatttc cttcttcatc aacatttata
    19741 ccaacgtcta taactaccgc tccatcttta acaaaacttt catcaaccat cttagctcta
    19801 ccaatagctg ctattagcac gtctgcctct gctgctacac ctgatagatt tttagttttt
    19861 gaatgacata ttgtaacagt tgcgtgttca cttaataaaa gcattgatac tggctttcca
    19921 actaccattg atcttccaag aactgttacc ttacttcctt ttaaatcaac attatagtgt
    19981 tttaatatct caactactgc tgtaggtgta catggtggaa atccactttt atccccttcc
    20041 attaccttag cagagtttat tggactaaaa caatctacgt ctttttccgg tgcaataaca
    20101 tattttataa cttcttcatt taagtgtttt ggaagaggtc taaaacataa tataccatta
    20161 acatttttgt catcattaac tctctttaat acatcaatat attcctcttg tgttatatcc
    20221 tcagctaact ctaacacttc tgttgttata cctatgtttt gacatctctt tagtgctcct
    20281 ctttcataag ataaatcatc acttcttgcc ccaactctaa ctattgttag tttaggattt
    20341 ataccttctt taattagtaa atcgacttct tttattaatt cttcactaat cttatcagct
    20401 acaggttttc cttttataat ttgtccttta gttgacattc cttccataca ttcacaactc
    20461 ccctacaagc aaacttaaat taatttattc tatataccac atattaataa catagtatct
    20521 actttcgtca tttaaaaatt attttccaag agctttttca actttggcat aaacttcatc
    20581 acaaattttt acaccatctt caactaactt atctacttct gtttttacct tattaacata
    20641 ttctttatct tgtatagaat ttatatttat aactacattt aattgtccac caagtatagc
    20701 agctcttaaa cactgaactc ctacaccaac atcacttata gcaagtcttg aacctttatc
    20761 aactaaatct tcatgtaact taatagcttc atagcaaact cttactatgt ttaatggtac
    20821 ttcacaagca acttttaatg ccttttccat tgtttctgct tttattcttt tttcttcttc
    20881 tgtttcttta ggaagcccgt aagcttttga taatggtaag aaacattctg catcatcatc
    20941 tatcattttt aataaatctt tttctaactt tcctgcctta cttaatatct cttttacact
    21001 ttcttcatac tcagcatact tcttcttccc tattgtaagg ttacatacca tactacctaa
    21061 agccatacct attgcaccta ctagtgcagc agctccccca ccacctggta ctgcttcttt
    21121 ggaagatagt acttctacaa aatcaacaca agttttatct gctattttca tgataatcct
    21181 ccttgaaagg atgtaggaaa atatctcaaa ttgttcgaga tattttacga actattttaa
    21241 gatatctcct actctttatt tataaattaa tttccttaga ataatccagc tattactcca
    21301 ttttcatcaa catctatttt ttctgctgct ggaacttttg gtaatcctgg catcttcatt
    21361 atttctccag taagtgcaac tataaatcca gctcctgctg aaattgttgc ttgtcttaca
    21421 gttattctaa atcctgttgg tctacctaat ttagtttggt catcagttaa agaatattga
    21481 gtcttagcca tacatactgg aactttagta aatccaagtt tttctaagtt agctatttcc
    21541 ttatcagctt caggagtata atctacacca tctgctccat aaatcttagt agctatagca
    21601 tttatcttgt cttttataga taaatcatct tcatagcaga atctaaagtt attttcttct
    21661 tcatccaata atcttaaaac ttctttagct actgcaattc caccttctcc accttttgcc
    21721 catacttcag aaagagcaac attaacacct aattccttac acttgtcttc aacaagttta
    21781 agttcagctt cagtatcaag tgggaatctg ttgattgcaa caactgctgg taatccatat
    21841 acttgagtta tgttttcaac atgttttaat aagtttggaa gtcctttttc aagagcttct
    21901 aggttttcgt tatttaagtc tgctttagca actccaccat tgtattttaa tgctcttaca
    21961 gtagcaacta ttataacagc atctggtttt aagtttgcca ttctacattt tatatctaag
    22021 aatttttctg ctccaaggtc agcaccgaaa ccagcctcag ttattacata atctgcaaag
    22081 tgcatagcca ttcttgtagc tataacagag ttacatccat gagctatatt tgcgaatggt
    22141 ccaccgtgta caaatgatgg tgttccttca agagtttgaa ctaggtttgg ttttaaagca
    22201 tcttttaata atgcagccat agcaccatta gcttttaatt gtctagctgt tacaggttca
    22261 ccttcgtagc tgtatccaac tattatgttt cctaatcttt cttttaaatc agatatatca
    22321 cttgccaaac agaacgctgc cattatttca gaagcaacag ttatatcgaa tccatcttgt
    22381 ctaactgctc catcaccttt tttacccata ccatcaacga tatttctaag ttgtctatcg
    22441 ttcatgtcta cacatcttct ccaagttatt ttcttagggt ctattctaag tgcatttcct
    22501 tggtgaatat ggttatcaag catagcagct aaaagattgt tagcagctcc tatagcgtga
    22561 aaatctcctg tgaagtgtaa gtttatatct tccataggaa caacttgtgc atatcctcca
    22621 ccagctgctc cacctttaac accaaatact ggtcctagag atggttctct taaagcaact
    22681 aatacatttt tgtctagttt agcaaatgca tcagcaacac ctatagtagt agtagttttt
    22741 ccttcacctg ctggagttgg gtttatagct gtagttagta ttaacttagc ttttttacca
    22801 gtctctcttt ttagtagatt gtagtcaact tttgctttgt atttaccata aagttctaca
    22861 tcatcttcac ctagtcctaa tttttttgca acctctctaa tatcttgagg cttagcttct
    22921 tgagctattt caatgtcaga tttaaatccc ataactgaat ctcctcccac aacctaaata
    22981 gatttaaaat ataatttttg taaaaattgt attaatatgt aaaaattata ttttataaga
    23041 taattataca tgtaaaatta cttaaaaact agctactaag ctattaaaat attcaaactt
    23101 aatagctagt tatttatcta attacttact tattacctat ctattgccta tgtctaacct
    23161 ttaaccaact taaaatccca aatcaaaatt tctatctatt ttatttatcc ttaagtgaca
    23221 ttaatttatc ttttattgct ttaatctgtt ctttcgttgt ttcactagaa tcatatggag
    23281 attgattagc tctatcagaa tcaataacat cttggttata ataaataaat cctaaagatt
    23341 ctccatcttc taagttttgg attataaatt cttcatcttc cttatttcta attttatttc
    23401 ctactacaaa tactttattt acacctatat catgtccaag tttcttaact tttctataag
    23461 tctgtagact tctctctcct ggttctacaa ctactataaa agcatctaca ccttgggctg
    23521 ttcctcttcc taagtgttca attcccgctt ccatatccat aaccactaca tctttatttt
    23581 gtagtattaa atgtgagcag agtctcttca aaagaacatg ctctggacat acacatccag
    23641 taccaccaga atctacggtt cccaatgtaa gtaatcttac tccattgtat tctttacaga
    23701 aattttcagg gatatcgtct actttaggat tcatcttaaa catttttcca aatgaaccta
    23761 cagaagcagc tgttctatct gaaactaatt ttttcatttc tgatataggt actattgatt
    23821 catatacttc ttttggaaaa cctagtgcta aagctaaatt agcatcaggg tcggcatcaa
    23881 cagcaacaac tctgtatccg tcttcagcaa acatcctaga gagcattgaa gaaaaagtcg
    23941 ttttacctac tccaccttta cctgttattg ctatcttcat aatccctacc accttttatt
    24001 actatttgct ttttagtagt taattttaca gttaaatatt ttttatactc tatattttta
    24061 tacactaaaa ataaactaca gctagtcctt acaggaacca attaaattta ccctactagc
    24121 ttttcaaagc agcatgtata gttttattat tctttaatat tgtttatatt ttatttattc
    24181 agtatattca ttatattttg atgttcttta ctttaatatt tgactttatt caactttatt
    24241 caaccagaaa cttcttataa aaacgtttat tctataactt cactattttt taagcttcag
    24301 ccttttctgc catcttttct tcaactaatg cttcaaagtg aacacgtttt ttctctatat
    24361 cttctattat tgtagctgct aactcttttg gatttgtatt tacagtaaat gtagctccaa
    24421 ctttatcttt taattcacta gttaatatat ccactgctct tgaagaacca gctattggag
    24481 gcattattcc taaataagtg tctataccag aagctacaac atagcatcct atagcaactg
    24541 ctttttctga catccactct ggagctatac ctacaactgg aaggtcacac atatccatat
    24601 ctaagtaatt agctgctgct ccaactaaat ctagtatacg acttatatca acacaagaac
    24661 ccatatgtag tactggtggg attcctacta atttacagac agttgcaaga cctttacctg
    24721 catatttttc agccgcatca gattccatta aaccaaactt agctgctgct gaagctccac
    24781 aacctgtagt aactactatt atgtcgtttt tgattaattc tttcattatt gttacgtgag
    24841 cctcatttga aactccttta gcattgttac atccaactac accagctgct cctcttaata
    24901 ctccagattt taaacagtct gttaatggct taactgttcc tgctgggtct atatgagagt
    24961 ttacaactct gtctaattga tttactactg cttctacact atacccaact gttgcgcctg
    25021 attttaattc aggtataagt acttttgatt tatctctatt tttaaagttt aaaatagctt
    25081 ctttaactat tgctttagca tcatcaagtg cttgttcttc tctaaattca atataagttg
    25141 aacctgttat cttagctttt ggagaagttg ttacaaattt agtatggtaa caatctgcaa
    25201 ctctagctaa tgcagggaat atacattgaa cgtcaactat tacagcttca actgctccag
    25261 ttattattgc aagttcttgt tgatggaagt ctcctgctat ttttacacca tgtctcattg
    25321 taatctcatt acctgtacaa cacatacctg ctaagtttat tccatcagca ccaacttctt
    25381 tagctaaagc tactaagtct ggctcttctg atgctaaaac tatcatttct gataatgctg
    25441 gctcatgtcc atgtaatatt atgttaactt tattttcttc taatactgct aaattcgcct
    25501 ctgttcttct tggcactgga gttccaaata agatatcgct tagacgagta cctatcatag
    25561 aaccacccca accatcagcc atagaagttc taagactcat gtgtattaaa ctatctatat
    25621 caccagtaca tcctatatga gtagagtgca ttattgttgc aatttctcta tctatagctc
    25681 ttggctctat agcgtattct ttccatattt tttgtcttgg ttctggtgca ttttttataa
    25741 atcttaatgt cccaaaaggt tttccaaatt ccattaatgc aatttctgcc acttcatgtg
    25801 ctacatcata gatatctcta ccttctgtct ctacatccca ttctttagct aaagtaatta
    25861 acttagcttc gtctcttatt ttatagtttc catttggatc agctaatgct aatgtatgtg
    25921 ctatatctct agcatggtct gaatgggcag ctgtcccacc agcaaccatt cttgcaaagt
    25981 ttctacctac tatagtatgc tcatctgctc cacataaccc ccttggagtc ttagggctta
    26041 ttctacatgg tcccattcca catattctac agcaaacacc ttgaagacca aaaccacatt
    26101 gtgccttaaa gccttctttt ctatcataca ttgtttctac gccttcttct ctagctttag
    26161 ctatcatctt ctggctgttt aaatcaattg ttaacatttt ttcatccata ttcctatccc
    26221 ctttttttga tttttaaaat cccccaggtg actgacttca tgtcataaaa taataaaata
    26281 aatgctttct aagttgatta tatactaatt ttcagtgctt tgctttagat ttaaatattt
    26341 ttttaacaaa attagataat acataaaaat tttattttct acacttaatt taagttgatt
    26401 ttatcgcttg tttttggcta tttgttaata tctaaaccta cttttttgat acttcagttt
    26461 aattaaatta tgttttgatt tttttaaatc aaatttgaaa tttttctagt catttttata
    26521 ttattgcaga ttgtttgtgt atttattatc tttcactact gtttggactt attacagatt
    26581 tttactccct aaacttaatt agtaaaaact tcttagaata gcattttttg gactaatttt
    26641 tttgaatttt tttatttttc ctactttaat attaattata gcttcaaatt gaacatttgt
    26701 atatattcat ttgaagctat aattattttt tatataaaac ttttttaata cagcattaat
    26761 ttagacattt tgactatttt ttctacttta attcatttat ttcaagcgat ttatcctatt
    26821 tcatattttt agctacaata gccccatatg caatagaata taatttagat tcatgtgaat
    26881 cagctcttga cactaaaact ataggagctt ttgcacccat aattatacct gctgatttag
    26941 agtctgccat atatgtaaag gctttcccta ttccattacc aacctctatt gttggaacca
    27001 atatgatatc tacatctcca gatacttcac ttttaaaccc ttttatctca ctagcttctt
    27061 ttgaaacagc taaatcaaaa gccagaggac cttctacaat tacattttca ccaaattctc
    27121 cttctttaca agcttttgct aacatatctg catctacagt tgcttgcatc ttaggattta
    27181 ctttctcttt agctgccaaa catgcaactt ttacagtttc cattcctaaa gcttttgcag
    27241 cttttataga attttttaat atctttgcct tttgttcata atcaggagct atattcatac
    27301 ctccatctgt agttataagt aatttatggt atttttctaa ctcatatacc ataacatgac
    27361 ttaataaact atcagttcta agaccatagt ctttatttaa cacttctttt aatatgacag
    27421 atgtatctaa taatcctttc attacaaagt cggcttcttt acttgatact aatttaactg
    27481 ctatttctgc acactctttg atatcatcct tatccactat tttgatacca cttaaatcaa
    27541 atcctatttc ttttgatatt tctcttattt tgccttcatt tccaaccaat ataggagtaa
    27601 ttatatttct ttctacagca tcctttattg ccattaaaac ttctttatca tgagctgctg
    27661 ctacagagag tacaattttc tctcctccct taagttgttc taaaatatca tctaattttt
    27721 ttatcaaaag taacccctcc cttatatata tttaactaaa taataccata tttgctatac
    27781 cttttctcaa tttatttttt atgttataaa gaatttatat aactaaaaga cagtttaata
    27841 ttttattctg ggatttcatc aataactctt aaaagctttg caggaacacc acctacaata
    27901 gtattttctg caacattttt tgttacaact gctccagcag cgataattgc attatctcca
    27961 atagtaactc ctggaactac agttacatta gcaccaatcc atacattttt tcctattgtg
    28021 attggagaag gataagttgt accacgtttc tctggtgcaa taccatggtt aagtgtagcc
    28081 aataccacat tatgcccaat ttgcgtatta tctccaatct caatcccccc ctggtcttga
    28141 aaatggcaac tgctatttat aaaaacattt tttcctattg tgatgttttt tccacaatct
    28201 gtataaaatg gtggaaacat aaaaaatgat tcatctacct tttttccaat taactcagaa
    28261 aataaactac ggacttcttc tggttcatga taagaagaat ttaacttact tgtgattttc
    28321 attgcttcat tactcaactt aaccataaat tcatgtattt ctgatttact agaaacaggt
    28381 tttctttgat tgatatgtgt taaaaattca ttgatattca ttttatacat cctttcaata
    28441 tgtgctttaa tttatttgta cattctaaat aatattttaa taaaaattaa tttaatttat
    28501 ttttattaaa atatcattgc ttcttttagt gtaatataca attatatcta tatcaaatat
    28561 ctatagtaaa tacttaacta tacttaaaaa gcatataaat tgatataatt atataataga
    28621 taagttaata ttgaggaggg ataattatgg aaataagagt attaagatat tttttagcaa
    28681 ttgcaagaga agaaagtata tctggtgcgg caaccttttt acatataaca caaccaacac
    28741 tttctagaca aattatggaa cttgaagaag agttgggagt taaattattg gttcgaggaa
    28801 atagaaaaat caccttaaca aatgaaggta ggcttttaca taaaagagca gaagaaatta
    28861 tatcattagt tgataaggtt gaattagaat ttgacaatcc caatgagatt attagtggtg
    28921 atatatatat aggaagtggt gaaactgaag ctatgcgact tatagctaga actgcccatc
    28981 attttcatca aaaatacccc aatgtaagat atcacctttt tagtgggaat gctgatgatg
    29041 tgactgaaaa attagacaag ggactgcttg attttggtat tttaattgaa cctgcaaata
    29101 tacagaagta tgattatgca caaattcctg ctactgatgt atggggttta ctaatgcgta
    29161 aagatagtgc ccttgcatca caaagcacaa taaatccaaa agatttatgc aatatacctc
    29221 ttattatttc aagacagaca cttgttgata aagcaatttc taaatggctt caagacagta
    29281 atgaaaaact aaacattatt gcaacataca atttagttta taacgcttct cttttggtag
    29341 aagaaggtct gggctacgct ctgtgtttag atagactcgt caatactaca gaaaacagta
    29401 atctttgttt tagacctcta tctcctcctt tagaagctaa attaaatatt atatggaaaa
    29461 aacatcaaat attttcaaag gcagcagaaa aatttttact tcaaatacaa aaagattttt
    29521 cttctatcat gttggataca tctaagttgg atacacctaa agaaaaataa atcataataa
    29581 aaggtgagag tgtctcaaaa tgaactttgc actgtataag gcactctttt ttatacaaag
    29641 taaaatatat gttcatcatt tcagcactaa aaaagaggct tatctctatt ttgagatagc
    29701 cccacttaac caataaaatt aaatacaact taacaatatc cttacttatt tattctaagt
    29761 tctcttagac aagcaatagt aatttcatct tgtggattta attcataagc tctttctata
    29821 tagtaagttg catcatcaat atcaccatta tttaaataag ccattcctaa attgcaaagt
    29881 atttctgggt cttcctttat cttagccgct tgttcaaaat actctattgc tttatctaaa
    29941 ttattcttat aagcctcaca aagccccaat tccactatag tatccacttg attaggcttt
    30001 attatcaata tcttctcaag atacatctta gcctgctcta tttcgcccat acccttatat
    30061 ccaagtgcta tcataaacag caagttccac caatccatat gttcttcttc taatggaagt
    30121 aacttctcta atccttcctt aaacttacct tggaatacaa gtgtatatcc ttcttcatat
    30181 tgaactttaa agtccatctt gcccagattc tcttgtacct cagctactaa atcagcatct
    30241 aaccctagcc ttaacgcttc ttcccaagtc agcttagtct ttaaatattg accttgatta
    30301 taataatggt agcctaagtg ataataacct aatgcaaaat tctcatcaac atcaattacc
    30361 ttttcaagct tagccatggc ttcaagtaag aatgcattca ttgccttatc atccatatct
    30421 ttttgatact gtttagctat ctcttggcaa acaatcgcat agttatacat tgcatctaaa
    30481 tcatctggat acattgttat aaatgctttt atatatatta aagaatctgt atgttcacca
    30541 atctcaaagt attttctcga catatatccc atatacgatt tcaaatccaa atcaatatct
    30601 tttgataaag catttaaaaa cttctcatac tctgcattgt ttttaaactt actatctatt
    30661 ccaattatat atatcattgc atctgctata gacattgaat ttaacttatc ttgagcagtc
    30721 ttttctttta tcccttttac taatacctca ttttttatag gtacatctaa tccacctttt
    30781 ggcacttcat agctttttaa tttaaactct ccaccatgtt ttatagttat aaaagctaat
    30841 tcttctgctt tttttagcaa atatttttct atcttaaaat ccattttatc ctcctaaata
    30901 cttaaatctc tactttatga gttttagatt tctcaaatat ttttttatca tcatactcaa
    30961 ataagactac attttctact tctcttattt ctgtatttat atcatttttt aaatccacaa
    31021 cattaaatct aaatggctca aaatgaacat attttattat atgtttagct ggtttatcaa
    31081 caaaactagg taaatatgtt gataagttat cattatcttg taaaaataca tggcatctat
    31141 tcttaaaatc atccacatct agcttattaa aaaactgtgg tatatttgat gttttaccca
    31201 gtgatatata gtcatatttt aatatatcgt tgaatacatc attatctatg ttgatttttt
    31261 cattatagaa atccatcaat attttatata actgattctt tccttgtgcc aaatcaaagt
    31321 agccattttc atcaaaatac ttggcaaaaa cctcaaagaa tttaaaaggg ctttttttat
    31381 aaaatctgcc tataatatat ctcatagata acacaaagtt tttagaattg tagtatctct
    31441 ccagtatatc ttctatatcc tttagcttca aagtttcttc atagcttata taattattgt
    31501 ataaaaactc atatggagca taatccttat atttaaaacc atgctcatct gcaacttttc
    31561 tcatacctgt ccctttaatc atctttaaaa agcctaattg caaatgctct atacctagat
    31621 taaatacatc attaaaagaa ttttcaaagc tcttataatc ttcataaggc agtcctgcaa
    31681 ttaagtctaa gtgctgatgt atatttctat aagatgctac agtttgtaca acatgagaca
    31741 actttttaaa atcatccctt ctacctactg catcaagtgt tttttcattc gttgactgaa
    31801 cacctatttc aaattgaaaa agaccttctt tacaatcagc caaaaactca agcatcttat
    31861 catctatcaa atacgctgta acttcaaaat ggtaagttgt atatccatta tcatttttca
    31921 taaggaaatc catgatttcc atagccactt ttctatttgc attgaatgtc ctatctataa
    31981 actttatctg tgatactctt gcatctatta aagcttttaa atcactcttt atcctgtcta
    32041 aactaaaata ccttagacct ggaatagtag atgataaaca gtattggcag ttaaaagggc
    32101 aacctcttga tgattcataa tacactattc tattttcata ttcttttgga ttcaagtttt
    32161 cataaggact aggaatctca tccaaatctt gaagtaattc cataggctta ttcactatta
    32221 tctcattgcc ttgtctatat acaagtccat ttacatcatt tatttccatc tttccttgca
    32281 agtgaagtac taagtctcta aaaattaatt ctccctctcc ataaagtata taatccacaa
    32341 aatcatactt tttcattgcc tcataactat cataactcac ttccggtccg cccaaagcta
    32401 ttttagtatt aggtctaatt ttttttagat tatcacaaag tttaactata tctcccacat
    32461 tccagatata tgttgagaat aatattatat catattcatt tttatatata tcctttaaaa
    32521 tatagtccaa atcgttattt atcgtgtatt ctttcatatt aacttctatc aaatctctta
    32581 caaattcttt tagatatctt atagccaaat ttgtatgaat aaacttggaa tttaatgttg
    32641 ttaataaaat tttcaaatat ttctcacctc tttttaagat tatactgtct ttttgtaccc
    32701 cttataggtt aaaatagtat atgtaaagtc taaataatat ctatataatt aatttattaa
    32761 tttcaagtaa tgtgaggaaa ggaagttttt tatggaatta attgaaaaag tatctatcaa
    32821 ctctttatca aaaagagatt tactacttat tataaagtct cttgaataca ctaatgaaaa
    32881 tactcaaatt agtgatttca ttgaccttag gaataatata gtaaaggaat tatgtttttt
    32941 aactgacact aaggaaaaag attttttaga ttatctagaa aaaaatagca cactctaata
    33001 acctaaacct ttttaaactt taatatataa atacctgatt tagaatttat ttttatgctt
    33061 aatatattat cattaaaggt tttcgacata actgttattt gattaaatat agggtctatt
    33121 atagtattac cttcttcaag tcttataaga cttcttgggt ctatgctttt attatatgcc
    33181 ttaactccaa ttttatattt tctcaattta acaacatatt cggacataga aaaaataaaa
    33241 tctatattat cttcatttaa tttactatct tcacccaaaa tggataatat agattttttt
    33301 atgctattaa ttccttcatt ttctacagtt ctatcttcca tatatacatc atatagaaca
    33361 tttaaaacat ttaataaaat actttcttca tcataatctt cagatttcaa atcaatgtac
    33421 ttatcaatta attgtattgc tttaatcctt gccatctgaa actttacaac atctgatttg
    33481 tctaattcta tagaaaatcc tattatcttg tctttaatac tctgtagtaa ttcgacatac
    33541 tctattttac tattatttat aagattgtgc ataatggaat tatacaccac agaacctaat
    33601 aaataatcaa ataaaagttc ttctttttta aaataaataa catacttttt tattagttta
    33661 ataacctcat cttcactttt ttgaatatta gtatttgata aaattagtgg taataaaata
    33721 tttaaaaaat tgatatttcc tattttttct ccataaaatc tatagtaaat aagttcatct
    33781 ctcagtctat tttcgtctat atacacacat ctttgcgttt tatcatctat taaggatttt
    33841 gtatttatta tagttaaatc tttaagagcc ctacttgcac tactaaaaga agatgtgtag
    33901 acttttttta gtaattcgtc attcataaat atatcctttc tgaaaaattg aatgtaaata
    33961 aattgttatt aaaaaaaaag agacagcccg tttaagaagc tgtcatatat agggggatag
    34021 tagtgtacaa aaatcattgt aatctactat ctatatatta ttatacgata ctactttttt
    34081 atatgtaaag catcagtttt cgacatccct atattttaca gttatacaaa ttgcgtacta
    34141 agttcaattt tattcttttt atttcaacat tttatcatgc tacattcttt caggagcgtg
    34201 cattcccaac aatttcagag aattttctaa agtttgtttt gttgctaaaa ctaatgcaag
    34261 tcttgctttt cttatttcca aattttctac taaaattgga ttgtcatgat aaaatttatt
    34321 aaacgcttga gctaaatcta gcataaatct agtcactata tgtggctcat ttttctttag
    34381 agcaagtaaa atattcttat tgaatgattc tataactcta agaacttctg cactatcttc
    34441 atcagctaat agactataat ctatatcaga agtcacttct acttctgctt ttcttaaaac
    34501 tgcacagcat ctagcatgtg tatactgaac atatggtcca gtctcaccat caaaactaag
    34561 cgttctttcc caagagaagg tatagtcttt aattctacta ttagataact cttggaatac
    34621 tactgcccca acacctactt gcttagatat ttcatccaca tttttagcat ttgggttttt
    34681 agctagtata atttccttag ttttatctat agcttgattt aaagcatctt ctaaaaatac
    34741 tactcttcct tttctagtcg acatagtacc ttcttcaagt gcaaccatac caaatccaac
    34801 atgaattaaa tcttttgccc aatcatatcc cataagctca attaccttaa accattgttg
    34861 gaaatgtaag ttttgttgag aaccaactac atatatacac ttgtcaaaat cataagtttc
    34921 ttttctatat attgcagcag ctaaatctct tgtcatatat aacgttgagc catcattctt
    34981 agtaataagt gctggtggca ttttatactc ttctaaatca acaattcttg ctcctttaga
    35041 ctcttttaaa agatttttct cttctaatag ttcgataact ctgttcattt tatcagaata
    35101 aaaactctct cctgctaaag aatcaaattc tatatctaaa agtttataca ctctgttaaa
    35161 ctcttttaaa ctctcatttc taaaccattg ccataattct tgtgcttctt cgtcaccatt
    35221 ttctaattta ttaaaccatg ctcttgcttc atcttccatt tctgggtgtt gttcagcttc
    35281 atcatggaat cttacgtata attttagtaa ttctggaatt ggattagact ctactacttc
    35341 tttttctccc cactttttaa aggccactat taacttacca aactgagttc catagtcacc
    35401 taaatgattt attcttatag tcttgtatcc ttgcgaatca tatatcttat ataaagcatt
    35461 acctataact gtagttctta tgtgccctat atggaaaggt tttgctatat ttggagaaga
    35521 atattcaact attacagttt tatcttttcc aaaatcacta tgtccgtaat tttctttttc
    35581 atctaaaact tttttaatta cagtttctgc taattgagat ttatttacaa agaaatttac
    35641 atatccgcct aattgtatta cttttgttat ctctccactt ggttctatac tttccgccaa
    35701 ttcacttgct atcatgtttg gagcttttct aaacactttg gctaatttaa agcatggaaa
    35761 agcatagtcc cccatatcct tatttggtgg aacttctata agtgcctcta tctcttcttt
    35821 cgataaatct tctatttttt ctttaaggca atttgatatt gccaccttaa aatcttgcat
    35881 tctgttgctc ctctctacat tgttcctggt ttatattgtg cttatctctt tatgttaaat
    35941 caaatttcgt gttttttcaa gtatttcatt acagtgtaaa acactttagt aattatgttt
    36001 tctgtatata ttaaaaatac tataattgca gtttttaatc acactattat tgatttttta
    36061 taagactttt ttctatttta ttgtttcttg tactatccag ttgactttat atactttttt
    36121 taatctttta tctattttca tacgtcaatg ctaattttat atacttttct tttaatcttt
    36181 tattaatttt tatacatcaa tgttaacttt atataagttt ttttaatctt ttatctgttt
    36241 tcatacatca atgtcagctt tatatacttt ttttactttt ttatctattt ttatacatca
    36301 atattagctt tatatacttt ttttaatctt ttatctattt tctataaatc tttttcactc
    36361 ttaactttta ttccttcttc ttctaatagc cttgctgtta tacctttccc tggtatcttt
    36421 ttacctgaaa aactaccatc atatattaga ttacatccac aagaggggct accctctttt
    36481 agtattgctt cgctacatac atataaattt gctattttta gagtttcatt agcccctctt
    36541 acaaagtttt ctgttacatc cttattatct ttagtaatta cttttccatc aactatctca
    36601 gcaggtggtc ttggtgtaga tagaccacct aactgctctg ggcatacaag tgtaaattct
    36661 ttgcctttta aatattcctt gactttttca ttatcattat tatcgccact atatttacaa
    36721 tttattccta ttaaacatgc agataccaat atcataaaaa tgtgctccta tacaaattct
    36781 taatatattc tcatacaaat tactaatgta ttcttataca aattatttta atttaacaat
    36841 tgttactcct gctccacctt ctccatattg accacctctt tgtgatttta catgtctatt
    36901 tcttcttaaa atgtcttgta gccctgcttt taatactcct gtacctattc catgtattac
    36961 tgttacagac tctaaacctg ccacatatgc atcatcaaga tacttttcta cttccattat
    37021 agcttcttct aggtttaacc ctctaaggtc aacttcattt tttacactac cagattttga
    37081 ccttataatg tttctcgttg atttagttac atttgttgaa acatctttgc gtgttttttg
    37141 taaagactta aaaggtagag tcatcttcat tataccaatt tgcactactg cttcttttct
    37201 ctttttatct actgaaacta cactaccatt ttgatttaaa gttattacct taacttcttc
    37261 acctggcttt aagtctttta tctccttatt tgataccttt ggaactatca tactttttac
    37321 tgttggctga agacttccca tagagctggt caattctttt ctaagttctt ctatcttacg
    37381 attcttttct tttgaagctc tttcttgttc aagacttcta agttctttta ttataatatc
    37441 tacttcttct ttagcttgtc ttataattga aaatgcttct gatttagctt tttcaatcat
    37501 cttatctctt tgactgacca atttttcaag cttttcgtca tactctactt tcagtttttc
    37561 aatctcttct tttaatcttt cagcttcttc tctatcttct actgctttga ttctattctt
    37621 ttcaacattc tgtaacacat cttctaaagc tatgttttct gtatttatat attctttagc
    37681 acgtgaaatc acataatcac ttagcccaag ttttctagaa atctcaaacg catttgattt
    37741 tccgggaaca cctataagta acttatatgt tgggcttaaa gtttctatat caaattcaac
    37801 tgctgcattt tctacacctg gtttagtaag agcataattt ttaagctcac tatagtgagt
    37861 ggtagctata catttagctc ctacagaatt tatatcttct aatactgcta ttgcaagggc
    37921 agctccttca acagggtctg ttcccgctcc taactcgtca aatataacta aagagtctgc
    37981 tgttacgttt tgtaatatag atactatatt cgtcatatga gatgaaaatg ttgatagact
    38041 ctgttcaata ctctgctcat ctcctatatc tgcaaataca ttatcatata cacacatact
    38101 acttccataa tcagcaggta tatgaagtcc actttgtgtc ataagtgcaa atagacctac
    38161 agtttttatt gtaacagtct taccacctgt gtttggacct gttatcacca atgtatggaa
    38221 gtctcttcct aaataaattg tatttgctac taccttcttt ttatccagca gtggatgtct
    38281 tccatttttt atgtttaaat atttatcctc atttaaagtt ggttctattc ctctcatctg
    38341 tatagaaagt tttccctttg aaaatgcaaa gtccaatctt ccaagtatct cttgattaga
    38401 aattaagtct tcactaactt ctccaaccat agcagaaagc tctgataaaa ttctttctat
    38461 ttcttcttgc tcacctagtt ttaactgtct caattcattg ttcatctcta ctatagtcat
    38521 tggctctata aaaagtgtcg caccagaaga tgactggtca tgcactatac cagctacttg
    38581 cgacctatat tcagatttta caggtactac aaatctatca cctcttagag aaattattgc
    38641 atcttgtaag tacttttgat aagtagttga acttattatt gaatttaatt ttgacctaat
    38701 ggattgattt ttttgggcta ttcttcttct gatatctcta agtgtacttg aagcattgtc
    38761 agatatttct acttcactaa ttatagcatt gtatatctgg tcttctatgt ccttgtatac
    38821 atatagagaa tttgatagag attgtattat tggataatta aaatcttctt catcactact
    38881 agataagcta tttcttaatc ttctagccac tcttaaggta tctgctatca taatcagact
    38941 tcctgggtca agagaagcac ctatataagc tctctttacc ttatcttcta tatcatgaat
    39001 accttccaac cctactgaac ctctctttat aataattgat tgagcttcac tagtttcttc
    39061 tagcatagat ttcacttcta caaaatcagt atttggtact aaattttcta tatattttag
    39121 acctaaagaa gatgaggctt tcttctttaa caaatctatt attttattgt attctagaac
    39181 tcttaacgat ttctcattca ttttctatat tcaccatcct cgatatttta acttttttat
    39241 ttaattttaa aaatgtatgt tttcaagcat ttccaataaa aactgcaagt tttatttctt
    39301 tttcatttct aaaattatta tattttttct cttttagata tattttacac atattttgta
    39361 ttatactatg tatgtagcta tctatatatt atacataaac atgctttatt tgtaatataa
    39421 tctgttaaaa aatcttcttt aatattttta aaggagttta ttttagctta taaaaaaaaa
    39481 tagctactta tatagataaa ttaaggagga acatgagtca tgggaaacgt attagaagga
    39541 ttaaaaccag aaagtgtttt taaaaacttt gagaaaatat ctcaaattcc tagaggttct
    39601 ggaaatgaaa aaggcataag tgatttttta cttagctttg gtaaaaattt aggtcttgaa
    39661 actattcagg atgaaagctt aaacataata ataagaaagc ctgctacaaa aggatatgaa
    39721 aattgtccag gtgtagttct tcaaggacat atggatatgg tttgcgaaaa agaaaagaat
    39781 gttgaacatg attttcttaa agaccctata aaattaagaa ttgatggtga catgatatat
    39841 gctactggaa ctactctagg agctgacaat ggtatagctg ttgctatggg tatggctata
    39901 ttagaagata atactttaga gcatcctgca ctagaagttt tagttactgt taatgaagaa
    39961 gatggtatga atggtgcaga tgcactagac cctagcttaa ttaaaggtca atatatccta
    40021 aatatggact ccgaggagga aggatactta ttagttagct gtgctggtgg taaaacttgt
    40081 gttgttagtt tacctgttga atacaaggaa gttaaaggtg acaaacaagg attattagtt
    40141 gaggttactg gactattagg tggacactct ggtatggaaa ttgttttaca aagagcaaat
    40201 gctaacaaag caatagctag agtattaagt gtattgaatg ttgattatga attagcttct
    40261 gttgatggtg gtactaagca caatgctata cctcgtgaag ctaaatgtgt aatagctgtt
    40321 aataaagctg atgttgaatc tgctaaaaaa caaataaatg acattttaac tgcatttaaa
    40381 catgagttta ctacttctga ccctggcatg acttactcag tagctgaaac ttctgtagac
    40441 aaagttctta ctaaagattg taaagaaaaa gttgttcaaa tgtcttgctt aactcctcat
    40501 ggagttcaat cagtaagtct tgatatagaa ggattagttg aaagttctac taacttcgct
    40561 ataatcgaaa ctaaagaatc tactattgaa tttttaactt ctgttagaag ttctgttatg
    40621 tctattagag atgaaatcgc tgatagaata agacttttgg ctcaagcttt aggtgctaat
    40681 tacgacctta tagctcaata cccagcttgg gaatttaaaa aaggctcaaa attagaaaaa
    40741 atatgcagtg aaacttatga gaaattaact ggtaaagttc ctactgttat ggctctacat
    40801 gctggtcttg agtgtggatt attacttgat aaacttcctc atgctgaagc tatctctata
    40861 ggaccagata tgtttgatgt tcatactcca aatgaacatg ttagtatccc ttctgtagct
    40921 aatgtttggg attatgttat tgaaatatta aaatctatga accaatatta aatttaagtt
    40981 ttgattctaa gtagatttta attttaagta atctatataa aattaactct gtaaattaat
    41041 actactgtta gtataatatt tactttcaat attaacttat agaaataaag cctaatgata
    41101 gataataaca tgtatcatta ggctttattg taatttaaat attattaagc tttattctgt
    41161 tttaaaatta attcttatac tattttaaat ctatttcttc cttgtctctt agaatcataa
    41221 agagcattat ctgctatttg atacagttca tcatactctt gaccaggctt tgctataact
    41281 acccccatac taatagataa actttctcca tttggcaact taatgagatg aatatgattg
    41341 tatacatcat tacatttatt aataagttct tccttattta ttatatcatt gatataaaca
    41401 ctaaattcat ctccacccaa acgacctact atatcatttt ttgcaaaact ttcatcaagt
    41461 acctttgcaa catcaataag tactttatct cctaacaagt gaccataagt atcattaatc
    41521 gacttaaaat aatcaatatc tataatcaat aaagcaccat ttttttcagt tgataaattc
    41581 aacaaatttt gtgcagttaa ttttctaata tatgctgcat tataaatatt ggttaaacta
    41641 tccctttgag ccttatctaa caatctattc ttttcctctt tttcagaatc aatattacat
    41701 attttcccga ttacatacat tggtattttt ctatcattat aaattatttt tgaagtaact
    41761 ctaatccaat gaaaagtatc atcaagcata atagaataca tttcagttgt tccattttct
    41821 tgtgatttta ttacatcaaa aaacttttta ttatattctt tccttatttt atctctttca
    41881 ttttctatat tgccattttc actttcctta tcatttttat ccaaaccata ataagtctca
    41941 ataccctttt ctggcataga aattatcaat ttatcttgct tataatcata ctcaaaaaag
    42001 tattcacttg tcatttcata caactgagta tgttttttga gttctaaaga tatcttctcg
    42061 ttcatacttg tacgagtatg caaaccaaat gccataaatc caataataca aattaataaa
    42121 ctggctacaa taaaaatagt ctcaattgga ttctgttgca taatataaag caacgagaag
    42181 tcttgtctat atgtagtatt ttggtagaca attgattgca ttttttctga tgaaatactt
    42241 ataatagctt tattaaatat attcaataat tcttgtttat cttttttaat tataccaaaa
    42301 caaatactct ttgctttata agtttgagga actagacgca catttttata cttaggttga
    42361 ttcatatagt actgcataga ataaccttcg ccataagtgt aatctgcata gcctttatta
    42421 acagcttcta tacactcttc taaattatca taccaaacta tattctcttc aaactgacca
    42481 taataattaa tacctttggc tagagctaca cgttttccat ctaaactatt ctcatttaat
    42541 ttattattaa tcatcatagt atattgagta gaaagaaaag gccgagacat aactatatta
    42601 tgttcacgag agtattcata atcataaatc attcctgcta caatatcaat ttcacgcttt
    42661 ttagtcatgt tttctaaatc ttcttgagat tttacaggca caagttcaaa ttctaagcct
    42721 gttttatcac atatatgatt taacaaatca attgatatac ctcttaattc acctgtatct
    42781 ttatctatat tttgaaatgg tggtttgttt aaaagtactc ctacttttaa tttttctgtc
    42841 tctttaatgt aatcagtttc ttcatcagat aaaaacattt gattgttctc tggtgtaaag
    42901 tatttttcat ataaagtagt tgaaaaatat gggtctttct gttcaatatt attaattgct
    42961 gagttaagct tttgagttaa atttgtacta cctttaggag taatgaaata aaatggtttt
    43021 gatgaaaatt ttgctacagt acgtacacct tctagtgggt tcatactaac attaagaagt
    43081 acatctgctt taccactctt tagtgcttct agttgctcat catttgtttt acaatatacc
    43141 aattcaggtg acataagatt cattttacaa tactcatcta actctttaag tctagtatca
    43201 gaattttcta ttacagcaat acggattttt ttcacatctt gtgaactcat tatgttatca
    43261 atatctgtat tttcatataa tgcttgtagt actgtatttg ctactccata actatttcct
    43321 gcataattat atattttttg cattggctca ttgtataaca tacctcccat tatatcaatt
    43381 tcaccatttt ctaacatctt cattaatttt gtaatagatt catttatctc tccaggaact
    43441 tgaacaaatt cataattcca tccagtatac tgtgcaattt ctagtaaata ttcatatgta
    43501 tacccaaaat agttaccttg ctcatctatt tcagttaaac ctttttgaat aggataagca
    43561 acacgaatgg tacgattatc attacctata gcgttgatac caaaagaaaa tatgcaagaa
    43621 gtgaatatag atgtaattac aataattaat aaaagtaatt tttttaaatt cactacacaa
    43681 tcctccatat agttaagatt tacagtataa attactgcat aaacttgtag acatgtaaaa
    43741 atagattagt atataaaagg atatataaat ctacttagaa atttaaatat ccttaatata
    43801 tacaatatat ttaaattata actcatttat atgtctatta caacaaattt accctgctaa
    43861 atacctactc tatatcccat aattttacaa gttgtagtga agtttaagtt ctatctcacc
    43921 tcaaatttag cagtgtttct atttacagtt cttttatatt tcacatctgg ataacccaat
    43981 ataaatgaag tgataacagc ttcgttctcg ctcattccta acagttcttt tatctttggc
    44041 tctacatttg atgccataac aaaaaatcca ttataacaaa ttcctaatcc ttgagaatat
    44101 gccataagtt ccatattaga tgcagccagt cctccatcta cataagcact tccacctaga
    44161 gacatatctc ctacaactac cataagtagt ggggcatcat aaaataaaga atcatttccg
    44221 ttctctttat atcttttgta cattttctta aatgtatttt tatagataga acgtacaggg
    44281 tcattgtcat cagtatctaa tgccaaatta taaagacctt gtattgccag ttctttcact
    44341 tcttttagct tttcttttac taatatgtat cttataggct gtctattacc acctgttggt
    44401 gtgtatctac cagcttccac tatgtttttt attttttctt cttctacctc tatatcttta
    44461 tattgtctta tacttcttct aaatttaata aaattcaata acttttcggg ttctaattca
    44521 aaactttcac gattgtattc tattacttca tttttatcaa atccttgcat ttctattgca
    44581 tctgttgggc aaattgccat acagtgacca cagttaatac agagaatatt tttaaccttt
    44641 gctttatcat ccttaacttt tattgcattt acaagacagt ctgattcaca catcccacag
    44701 cctacacatt tttctaaatt tatgtctatc attccaaatt attcctcctt ttaaaaaatt
    44761 tactttagta taaaacaaat agttgtctaa gtcaattttt atttttttaa caatatacta
    44821 gccttaacta cactttaatg gagaaaaata tacataaatt ggaacttaaa ttagaactta
    44881 ttcaaagtaa aagataaaag aagtagatgt ctaaaagtga actttttagt taattagata
    44941 ttctttattg tatgaaatca agtggatatt tatcatttca acaataaaaa aggcttacct
    45001 ctattttgga tatgtccctg tcaagtagac agactaaaaa aaggctatct ttatgcactg
    45061 agatagcccc ttttcccaac tactattgtt tatcttaata ttagtcaata aatttaattt
    45121 gctataacct gtaagttaca ttttaatttg atgtaacttt aaattacatt tttaagtaat
    45181 atctttaaat tatattctta aaatatatca ttaaactaca ttttaattaa tctaaaaact
    45241 atatcttaat taatctaaga aacttacatt ataatctttt agtttcccat tatcatatac
    45301 aatctttgca ctaaagatat ttccagtctt agcccaattt aagaacagcc tgtcaccttc
    45361 ccacatattt aaatcgaata ttttatcatt atcaacccaa attaaatttc cttctttaca
    45421 atcatttaaa tctttttcag ataattcacc ttcaaactca tctgagaaat acaaaaacat
    45481 ataccaatct tcttcatctt taaattttgg aaaagttatc aaccctttat atgacaatga
    45541 tttaagagta agaccagtct cttcttttac ttctctaatt acacattctt ctggagtctc
    45601 tccttgctca aactttcctc caactcctac atacttacct tcatgtatat catctttctt
    45661 tttatttctg tacaacatca aagtcttgtc atctttttct atataacaaa tagttgtaag
    45721 tttcatgtat ctcgccttgc tttctcttta tattctcaac taattatttt cagcactttc
    45781 aagcacatca cttatattct tcattgcatt aagagaaagc tctgcaaatt catcaaatgg
    45841 aatccctata tcttctatac ttctcattgc atctctattt acatttgaag caaatctcgt
    45901 ttctttcatt cttttaataa cagacttagt ctttacactg gtaagttttt tatctggata
    45961 aactaaagct atagcagtta taaacccagt tatagggtct gctgcatata ttgctttttc
    46021 cattaggcta gttcttgtaa ctcccattcc atcatgatga ccaagtattg ctctatacat
    46081 ttcttcgtca ccgaagtttt cttttttaag cagttctact gttgttgtag catgtccctc
    46141 acatatttct cctggctttc ttccaactat atctgaatcc aaatcatgta aaagacctgc
    46201 cactgcccac tcttcttctt tttctggctc tagtcttttg gctaattctc tcataacagc
    46261 ttcaacagca taacaatgtt ttctcattct atcagtttct aaatacttaa ataaaatttc
    46321 taaagcttct tctctcattt tcaccaatcc ccctgttttt tcacatatat gatattttta
    46381 tatattaata cacataaatt caagttttta tatatattca tgtatatttt tcatgttata
    46441 tctattctac acctttatag tagtgtcctg caactgttcc aatttcatct aatttcttat
    46501 acaagtctgt agcccttttt ccaagtttat aattattact caatacctca atgtgagctt
    46561 caataacctt tttaaccatc tcactatctt caactgtaaa atctatccta aatacatcta
    46621 ttccaatttc ctctaactca tatagattgt ccaacataca agtaaccttc gaattgtaaa
    46681 ttgtacttct acagaatata tcttgtgtta tcctataact tgcatccgta aagtctctta
    46741 aagcataact actttcctta catttagcaa ctctcttgtc ctttttacaa tccctaacaa
    46801 ctactcccat aggacagtat tctgtaacca tcataggagc atatccatat attactgctt
    46861 caatttctac atctgtatat tttaaagttt gtttaatttc atttaagttt agttcttgtg
    46921 acaaacaaac tgtacttgct ccctcttctt tgaagtggtt tatactttca ctattgaatg
    46981 catttaagta gcaatctata tttaagtctt ttttcttaaa gtattccata gagccccagt
    47041 taccaatttg aaccttatta attcctttta tattatccaa tatctcaaat tgcttgtact
    47101 ctttatttct aacaatcctt ggtgctgaat agattaattt tttgtcagtg actgctgtat
    47161 tagagtggtt cttattcaaa gtatctattt tacttgtatt tgacaaagta tatatgttat
    47221 ttgtatttga acccattagt tctttaggaa tacctaaaga tgaagaagta tataaatcca
    47281 tagaaatact tgaatctata gaaatacttg agaccatttg taaagctttt gataatgtat
    47341 ttatatcttc ataatagatt aaatctacat tataatttaa tactgcttct aattgctcca
    47401 agttcttaac tttaactcta atcttaggaa ctgtatcaag ttttttctgt ttatttactc
    47461 tatttggctg gtattttaat ctattcttct taaattttct atcttttatc tttattcttt
    47521 cttcatctaa aagctcaata caatcccttc tcatctgatt taacacactt ataggcatac
    47581 ttacaccttc atccaggtca atttctatct cttttaactt atatggtgta tttccaagct
    47641 tttgaatttg tgtttctatt ttttctttac ttaaagccac ctttatagct ttttcaacta
    47701 ttttgtctcc ttctaccacc gatttatttc cacaaaaatc acttataaaa agctctggct
    47761 ttttacctag tttaatactc acttttgcac gtatattagt ttttatgaac tctttatctt
    47821 gctgaaatgt agcttgtact ctatccatca attcgctgtc agaagtctta tatataagct
    47881 gatttctctt tgcttctcca acaaaatcta attctatcaa atccccttta tgtgctattg
    47941 tagttatctc tccatccttt attattcttc caatagtacc tccacctaag ttaattccat
    48001 cacctttctt aagcgtattt tctagcatta ctctaagtct tttgttcttt ctattataat
    48061 ctaaaacttt tccaatatat agcccttgat tattaggtct attagagttc attatatcct
    48121 taccaacttc acctaatata tatccttttg taaactttct attaaatatg gtatacaaat
    48181 tatttattat ttcctcagaa atacttattt ttttcttatc tacaaattca ttaatcgctt
    48241 ctctatatcc tgaaaccaca gtagctacat actctggctt tttcattcta ccttcaattt
    48301 ttaaagataa tacacctgct tcaattattt tatccaaatc ttctatagta cttaaatctc
    48361 taggactcaa caaataatct ccattacttt ttactacttc tcctgtattt atatctatca
    48421 actcatactt ttgtctacat ggctgtgcac acctacctct atttcccgac ctattgccta
    48481 tcatactact cataaggcat tgacccgagt aacatacaca taaagctcca tgtacaaaaa
    48541 cttctatatc tacagttgta ttttcacaaa tccattttat ctcatcaaca tttaattctc
    48601 ttgctaaaac aactctatca aaccctacac tctgaaggta ttttacatct tctaaggaat
    48661 gtgctaccat ttgagtgctt gcatgtagtt caaagtcagg taatagtttt tttataagtc
    48721 ttgccattcc tatatcctgt actattaaag catcaatatc tatatcgtat aaaaatttta
    48781 catactctac aaaatcttct atttcatact gttttatcaa tgtatttaca gtaacaaaga
    48841 cttgcactcc ccttatatga gagtatttaa cagcctcaat cagctcattt ctatcaaagt
    48901 tatttgcaga tgctctagca ctaaagtctt taccacctaa atacacagca tttgctccgt
    48961 tttgaactgc tgcttttaat gaatcaaaag agcctactgg tgcaagtaat tctatttttt
    49021 tcatattttt ctcctctatt tatacttgtt aggttaatat aattatatct ataacttaaa
    49081 tatttgtatt aaatttatat atttcttact atattttaaa ttcttcaatc aatttatata
    49141 ttagttattt atatttggtt catttgagtt aacatataca ttatttacta tatttagtat
    49201 atttctatta acttatatat tgattactgt attttaaatt cttcaattaa tttatatact
    49261 gcttactaaa tttaaaacat tttaattaat ttgtacactg tttactatac ttgaaatact
    49321 tctattaatc tatatattgc ctactatact taagacattt taattaattt atacactgtt
    49381 tactatactt gaaatacttc tattaatcta tatattgcct actatactta agacatttta
    49441 attaatttat acactgctta ctatacttaa aatacttcta ttaacttata cattgcttac
    49501 tatattttaa attctttaat taatctatac aatatttgct atatataaag tatatcaaat
    49561 aacagcttca tttttttgta aatttacaga aagttaattt tcattaaatg aatcatcttt
    49621 attgataaac ataattccaa agaccactgc caaaataaaa aaacatatta gtattgattt
    49681 ttccacgaaa atttttgtaa aaaaagttcc taaaatagct ccagatataa agaatataat
    49741 aatagcataa tactgtaaac tcttttttat cataaaaata ttcttagtat gtatataatg
    49801 aaataaagtt tctgtagcac ttcttaaatt tccagtacac attgtcgttg caaaactatt
    49861 cccatttact tttctaaaac tctctacttg cattgaacaa ataaaagaaa tagcaacatt
    49921 tacaaacata ttaaatcttc ctctaggaat aaatgaaaca ataagtattg taatcatttc
    49981 aattataatg ataacctgac gccaatgaat ctttgtctta ttatttgtaa aatactttct
    50041 tatcttctcg gctataaata cacctaacat aaatgccaaa ataggaatca aataataaaa
    50101 tgattccttt acattacctt ctgctatatt aagcccaaat aaaactatat ttccagtttg
    50161 tgcattagca aagactttac ctcttaccac ataagtataa atatccaaaa atcctcctgt
    50221 tattgccaac aaagaacata aaaagaatga ttcagacatc tgactcttgc ttgtcatata
    50281 taaacctctc gctttctaat gatagataca aaatcaaaat cattttagat tctatttttc
    50341 attgatataa aaaaattatt tatatttaaa tattgtctat atttatacat tatttgtatt
    50401 tatatagcat tcatatttat ttatatttaa acattatcta tatctatata gcatttatat
    50461 ttatttacat ttaaacatta tctatattta tatagcattt atatttattt acatttaaac
    50521 attatctata tttatatagc atttatattt atacattaaa agaagctgtc tcaaaaaaca
    50581 agagacagca aatcttttag tttattccac attttttgca tcattattta accctgtaca
    50641 ttttaactgt aaatcataag ctttgttttg aaactcagaa gctaagtttt cagctatagt
    50701 agctctttca tttgcttctt caagttgagt acttaactcc tcaatcttgt ttttataagc
    50761 atctaactct gctctagaac cttctgtcgt attactaagt tcgaatattt cttgcttttt
    50821 attttctata atctcaatta actctttcat cttagtttca gcttcacgat tttctgcttc
    50881 tttttctgct atcgtaagct ttaaagattt gatttctaat tgaagctctt cattagatgc
    50941 tccaaccttc ttactcaatt cttcattcgc tttaatcaac ttctcattct catctgaaca
    51001 ttcaaataaa aggtctgata tatttaatgc agttaatatc gcagcaactg ataagctcaa
    51061 tttcgggttt tgacgtgtta ttttatccat ttctttgtct acaaaatcag caatactaat
    51121 cataaacttc tctgatttgt ctccaaccat tggatattca gcgccatgga ttttaaccat
    51181 tactttgttc atcctaacct cccccgacat tagttgttaa tttgatctta agttagcatt
    51241 taatttttcg cttaattcac ttaatatctt atcatgcacc ttagctacat cttcatctgt
    51301 tagagtttta tctttacttc tataagttat tgaatatgca attgacttgt atccagcttc
    51361 tatttgagaa cctttgtaaa catcaaataa cttgtagctt tctactaatc cttgaccatt
    51421 ttcctttatt atatcttcta tttgttttac aaatacatca tctttaacaa taagagctat
    51481 atctcttgat gtagatggat attttggtaa tggcacataa ttctttgttc tatcagaatt
    51541 atcaaataca aagtcaatat ctatttcagc gacataaact ctttgaccta gattgtagtt
    51601 ttctataaca tctggatgca attcacctaa tgtacctaca tatttattat tatacactat
    51661 tttagcacat ctaccagggt ggaaggtagt attattatct tgaggttcaa tttcataacc
    51721 tttaaatccg acatttacaa gtatagtttc tatagttcct tttagtgcaa agaagtcaac
    51781 atctttacca tacataccta cacacattct attttcttcc ttaggtaatt ctgaatcttg
    51841 tgggatgaat atatgtccac actcaaatgc agatacttct tctattttat gagatacatt
    51901 agttgataaa acatctaaca tatttggtat taaagtagtt ctcattacag aagtttcttc
    51961 tcctaatggg ttaagtaatt taacaaaatt tctctttgca ttaccttctg gtactcttat
    52021 cttatctact cccttagggc ttacaaatga atatgttaat atttcattta atcctacagc
    52081 agttgaatta tcttttaaag catccatgaa tttttgtttt gaagttttaa ttcctgctgt
    52141 cgtatttcct tctaattgta cagaaggtat attctcaaat ccataaattc tagctatttc
    52201 ttcccataca tcagcttctt gttccatatc tgttctaaag cttggtacat ctatttctaa
    52261 tttatcatta gctaccaaat tacatttaaa ctctaatgat tctaaaattc ctacaaactg
    52321 ctccattggt acatctacac ctaataggtg gttaattctt tgaggattta ctaccaattt
    52381 tttaggttct ggtttatttg gatatacatc tacaacacct tttaaaactt ttcctgctcc
    52441 taacatttca acaagttgtg cagctctatt tgctgctatc tctgcaagat tagggtctaa
    52501 gtctttttca tttcttgaag atgcttctga cctaatacca acttttttag ctgtcattct
    52561 tatgttttct ggtttgaaat tggcactttc aaaaagtaca gtcttcgtat tagaagttat
    52621 ttctgagtta gcaccaccca ttacaccagc taaaccaagt gatttttctc catttgttat
    52681 aactagcata tctttgtcta atgttctctc aacatcatct aatgttacaa gtttctctcc
    52741 atctttagca tttcttacta ctattcttcc agtctctact tgatttatat caaaagcatg
    52801 aagtggttga ccaagctcta acattacgaa gtttgttata tcgactatgt tacttatagg
    52861 tcttactcct gcttctgtaa gtcttctttg catccaatat ggagaaggtt ctatttttac
    52921 atctgtaacc attctagcaa catatcttct acataaatct ggattatcta tctcaacttt
    52981 gaaatctatc tcttcgtcac tttcttttac ttcgatttca ggatatttta cttttgttcc
    53041 tatagttgca gctgcttctc tagctatacc catcatgcat ttacaatcag gtctgtttga
    53101 agttaattca aaatctatta aagcatcttt taatcctaaa acatctttta tatcttttcc
    53161 taattcataa gaatcttcgt ggtctaaaat atatatacca cctcttttat actcctcaat
    53221 gtaatgttca tctataccta gttcagctgc tgaacacatc ataccatctg ataattcacc
    53281 tctgaaatca gtctgtttga tttcaactcc tccaggtaac ttagaaccat ttacagctac
    53341 tggaatataa tctccttctg atatatttgt agctcctgta actatttgaa caactttatc
    53401 tccaatatct actttagtta caaccaactt atcagcattt ggatgttgtt ttatttctaa
    53461 aatctttcca accaatatat tttctatttc ttcaccataa taatctattg tttcaacttt
    53521 agttcctgtc attgtcattt tatcagcgaa ctcttttaca tccatgtcta tatcaacata
    53581 gtctctaagc cattttaaag atactaacat ctacataccc tcctaattaa aattgattta
    53641 agaatctcat atcattttcg aataataatc taatatcatc tatttcgtat ttaagcattg
    53701 caagtctttc aaccccaact ccaaatgcaa atccactgta aacttctggg tctattccac
    53761 aatttctaag cacatttgga tgaaccatac ctgaacctaa tatttctatc caaccttcat
    53821 atttacacat tgggcaacct ttaccaccac atttgaaaca agtaacatca acctctgcac
    53881 ttggttctgt aaatgggaag ttgtgaggtc taaacttagt tttgatatca gaaccaaaca
    53941 atttttcaac gaagatatcc atagttcctt taaattctgc catagtaaca tcttttccaa
    54001 caacaagccc ttctatctga tggaacattg gtgagtgtgt agcatctggc gagtctgacc
    54061 taaaacatct acctggtgca attactttta ttggtaactc ttgacttctc atagttctta
    54121 cttgaactgg agatgtttga gttctaagta ataccccatc attgatatag aatgtatcgc
    54181 tcatatctct tgatggatgg tctttaggag cgtttaatgc gtcaaagttg ttttcaacag
    54241 tctcaacttc tggcccttct gctatagaga atcccattcc gataaatata tctgttactt
    54301 catctataat ttgagttatt ggatgcttct tcccaacctt taaaactttt cctggttgag
    54361 taacatctat cacttcctct tttaattttc tttctttttc aatagcattt atttcttctt
    54421 ttttagaatt tatgctaagt tcaatgtttt ctcttacctc attggcaacc ttaccaacta
    54481 ctggtctttc ttcagcagat aatttaccca tttctttaag tatggcagtt atctcacctt
    54541 tttttcctaa gtacttaact cttaaacttt ctacactttc tatgctttgt gcttctttta
    54601 tttcagccaa agctgcttca cgtaaagcaa gtaatttttc ttgcacacat atcacccttt
    54661 cgtttttttg tataaaaaaa gcccctcaat ccctgtctag ggacgaaagg ctatagtttc
    54721 gcggtaccac cctagtagtt taacaattta aaaatcatca aaccacctct aacaaactta
    54781 acgcgttttt gacgtcaaag cctactgtaa aatttcagct ctgttactcc agagggaact
    54841 tctcatgacc tcttagaaaa cactcccagc ctaggtgttt atctctttac taagtttaat
    54901 caaaatactt tcctctttca ttgcatttac aatattatat tgtttatttt agtattatat
    54961 catttccatt caaattatgt gactattttt aatatatatc caatacataa ttaat
//
LOCUS       DAYASV010000012        51968 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYASV010000012 DAYASV010000000
VERSION     DAYASV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.012"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(154..2217)
                     /locus_tag="ACGI68_000759"
     CDS             complement(154..2217)
                     /locus_tag="ACGI68_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFH0137208.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYSNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(2598..5933)
                     /locus_tag="ACGI68_000760"
     CDS             complement(2598..5933)
                     /locus_tag="ACGI68_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFH0137209.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIGEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMMDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKAETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTENFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(5983..6411)
                     /locus_tag="ACGI68_000761"
     CDS             complement(5983..6411)
                     /locus_tag="ACGI68_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137210.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(6401..6781)
                     /locus_tag="ACGI68_000762"
     CDS             complement(6401..6781)
                     /locus_tag="ACGI68_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFH0137211.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYESKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(6814..7986)
                     /locus_tag="ACGI68_000763"
     CDS             complement(6814..7986)
                     /locus_tag="ACGI68_000763"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFH0137212.1"
                     /translation="MEYIKLNELCYINIGKTPSRNTSDYWGSGNRWLSISDLKEKYIS
                     KSKEEITDLAVEKANMKLVPKNTVVMSFKLSIGRVAILKEDMFTNEAIANFQIKNNEL
                     ITSEYLYYALRTLNFNNTDRAVMGATLNKSKLNDIKIPYFTICIQNKMVEVLNKAQEL
                     INKRKEQIEALDELVKSRFIEMFGEPINNTKKFDMKKIKEISIYLKRGVSPKYVEQSN
                     IKVINQKCIYWRNLKVENCKYYDENLKEKIEDIFLKQNDILINSTGTGTLGRCVKFNK
                     IDNSEYIADSHVTVLRSISNNLNSDYFAALFEFTNIQDTIYRKCVNGSTNQIELSVSK
                     LGEYLIMVPPIEFQNQFSEFVKQVDKLKLKIETNLKELEDNFNSLMQKAFKGELFN"
     gene            complement(7990..9453)
                     /locus_tag="ACGI68_000764"
     CDS             complement(7990..9453)
                     /locus_tag="ACGI68_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0137213.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFERIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KDSAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIAVGLEELERMLEV"
     gene            complement(9963..10832)
                     /gene="gatY"
                     /locus_tag="ACGI68_000765"
     CDS             complement(9963..10832)
                     /gene="gatY"
                     /locus_tag="ACGI68_000765"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFH0137214.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIRDVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(10852..12768)
                     /locus_tag="ACGI68_000766"
     CDS             complement(10852..12768)
                     /locus_tag="ACGI68_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0137215.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGV
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVAKLNNEEIKSV"
     gene            complement(12771..13691)
                     /locus_tag="ACGI68_000767"
     CDS             complement(12771..13691)
                     /locus_tag="ACGI68_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HFH0137216.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLADISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(13793..14545)
                     /locus_tag="ACGI68_000768"
     CDS             complement(13793..14545)
                     /locus_tag="ACGI68_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137217.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(14937..16382)
                     /locus_tag="ACGI68_000769"
     CDS             complement(14937..16382)
                     /locus_tag="ACGI68_000769"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0137218.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(16404..16757)
                     /locus_tag="ACGI68_000770"
     CDS             complement(16404..16757)
                     /locus_tag="ACGI68_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0137219.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(16786..18018)
                     /locus_tag="ACGI68_000771"
     CDS             complement(16786..18018)
                     /locus_tag="ACGI68_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0137220.1"
                     /translation="MNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMVFVGSL
                     VTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMETMKNKKFESMSVVAGLAGA
                     GLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGEDSALP
                     DFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFVLLNFL
                     VVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWITLGGL
                     GATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPMWIGTV
                     VISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFMVNQDVRGLIVLAINIVLLFMIWY
                     PFFKAYEKEEIKLESQEV"
     gene            complement(18041..18358)
                     /locus_tag="ACGI68_000772"
     CDS             complement(18041..18358)
                     /locus_tag="ACGI68_000772"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0137221.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            18600..20513
                     /locus_tag="ACGI68_000773"
     CDS             18600..20513
                     /locus_tag="ACGI68_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0137222.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGAFILSSKKGYKVNLNTNCQTKIDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     ELFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLVGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IDSVEVVNYIDNNVDINIAKDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYNQM
                     DTKSLSNYIDEHFVKLTKKIIKNVYDLYLIDLSDEEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQTLENVDIDEDEISYISLHVGSFFDRQKLLED
                     KVLCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIN
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(20636..21790)
                     /locus_tag="ACGI68_000774"
     CDS             complement(20636..21790)
                     /locus_tag="ACGI68_000774"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFH0137223.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKETIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDKNNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEKLDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLDSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(22069..24684)
                     /gene="mngB"
                     /locus_tag="ACGI68_000775"
     CDS             complement(22069..24684)
                     /gene="mngB"
                     /locus_tag="ACGI68_000775"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFH0137224.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPDRKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHQEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLIAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEDWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKEEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(24828..26753)
                     /gene="mngA"
                     /locus_tag="ACGI68_000776"
     CDS             complement(24828..26753)
                     /gene="mngA"
                     /locus_tag="ACGI68_000776"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFH0137225.1"
                     /translation="MEFKKITNENLVFLKQKIKTKEDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVELI
                     FLIAVPESEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AILLIITRKQKNQRKIH"
     gene            complement(26883..27608)
                     /locus_tag="ACGI68_000777"
     CDS             complement(26883..27608)
                     /locus_tag="ACGI68_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137226.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEAAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(27871..28641)
                     /locus_tag="ACGI68_000778"
     CDS             complement(27871..28641)
                     /locus_tag="ACGI68_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFH0137227.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(28643..30091)
                     /locus_tag="ACGI68_000779"
     CDS             complement(28643..30091)
                     /locus_tag="ACGI68_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0137228.1"
                     /translation="MKRFLNKMMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVP
                     AISNISNIVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNA
                     GVFGGIIVGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIY
                     PQAAMNQFGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVI
                     RGEIPRFMAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALT
                     SFMATVTEPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSF
                     SYGDKAWLVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLS
                     HGNFSYMAKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLS
                     NTSVQIIIGTDVVKVKDEMEMQLDELRKQADA"
     gene            30461..31183
                     /locus_tag="ACGI68_000780"
     CDS             30461..31183
                     /locus_tag="ACGI68_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0137229.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            31380..33047
                     /locus_tag="ACGI68_000781"
     CDS             31380..33047
                     /locus_tag="ACGI68_000781"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFH0137230.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(33143..34462)
                     /locus_tag="ACGI68_000782"
     CDS             complement(33143..34462)
                     /locus_tag="ACGI68_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0137231.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(34503..35249)
                     /locus_tag="ACGI68_000783"
     CDS             complement(34503..35249)
                     /locus_tag="ACGI68_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFH0137232.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(35493..37502)
                     /locus_tag="ACGI68_000784"
     CDS             complement(35493..37502)
                     /locus_tag="ACGI68_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0137233.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLGDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(37940..39373)
                     /locus_tag="ACGI68_000785"
     CDS             complement(37940..39373)
                     /locus_tag="ACGI68_000785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0137234.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(39460..41376)
                     /locus_tag="ACGI68_000786"
     CDS             complement(39460..41376)
                     /locus_tag="ACGI68_000786"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0137235.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGGVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQILITFDIESISKEGYCLETPVVVTNYSDYVDILENTQ
                     KHVNNSQELITVLA"
     gene            complement(41450..42310)
                     /gene="licT"
                     /locus_tag="ACGI68_000787"
     CDS             complement(41450..42310)
                     /gene="licT"
                     /locus_tag="ACGI68_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0137236.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(42661..43848)
                     /locus_tag="ACGI68_000788"
     CDS             complement(42661..43848)
                     /locus_tag="ACGI68_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137237.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(43862..45253)
                     /locus_tag="ACGI68_000789"
     CDS             complement(43862..45253)
                     /locus_tag="ACGI68_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFH0137238.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIVPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQELMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLRL
                     EWYITEIAMIFTAIGIISGIASGLKQDEIVNSFISGAGDLITAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     regulatory      complement(45397..45576)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(45848..47500)
                     /locus_tag="ACGI68_000790"
     CDS             complement(45848..47500)
                     /locus_tag="ACGI68_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0137239.1"
                     /translation="MKDKLQVLSGAMMIPVILLVVAGVFIGLGSAFANMENVSALGLS
                     GLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKKEKGWGALGGIVLFIGM
                     HTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGSFLGIFSYDCSIFGAII
                     AGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLGTAMYFIWPPVGLALSK
                     LGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVGGTMEVGGAIYEGVNNI
                     RLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLADNANKKKAISILIPAIV
                     TAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFNVSIMGESFRNMFPNLL
                     QPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSEEVKLYSKKEYRERQSV
                     DAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNCATRLRVELNSIDGFYE
                     DGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD"
     gene            complement(47520..48923)
                     /locus_tag="ACGI68_000791"
     CDS             complement(47520..48923)
                     /locus_tag="ACGI68_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137240.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELDEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGMYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(49187..49678)
                     /locus_tag="ACGI68_000792"
     CDS             complement(49187..49678)
                     /locus_tag="ACGI68_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0137241.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(49704..50516)
                     /locus_tag="ACGI68_000793"
     CDS             complement(49704..50516)
                     /locus_tag="ACGI68_000793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0137242.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(50529..51482)
                     /locus_tag="ACGI68_000794"
                     /pseudo
     CDS             complement(50529..51482)
                     /locus_tag="ACGI68_000794"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
     gene            complement(51606..>51968)
                     /locus_tag="ACGI68_000795"
     CDS             complement(51606..>51968)
                     /locus_tag="ACGI68_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0137243.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIKNRKLSRSISDVSWSEFIR
                     QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT        16613 a         9539 c         5492 g        20324 t
ORIGIN      
        1 atttttatta ataatttaat tagatagtat ttgtattact ttaattaaac gtaacctctt
       61 gatataaatt tgagctataa atctatatca agaggtgcta ttttattttt aatataatta
      121 atattctatt acgcaaaaaa tgcatatatt tcattatttt atggaaaata ttacttcatt
      181 taactctata tcaaactcat taagtaaaaa tctaattata tttaatttag cctctgtatt
      241 taaatttgtt tctaaatata tattttctga tattttaata ccttttctta atttatatgc
      301 atctctagac ttaatattat ctataaaatc tcttttataa gttttgtcta caatactgtt
      361 aaaccttaca gaatccaaat catataaagt aagagcaact tgttcataaa attctgtcca
      421 atttttagcc ttatatgact ctcctaatat actaaatgat actatacttt catttgtaaa
      481 attaacatca tcatcctcta acgtaaatat attttcactg ttacttggag gttcatatgt
      541 aacacttgga tatttccaaa tttctacagc tctatctagt aatatcttag ttctattttt
      601 tatttcttct tcattccatt tatctataga tgaaatatat ttatttagat aaagcctact
      661 atctttaaat ccatcttcca tttctttctt ttcttcaaat gttttattac ttagttttga
      721 attatatcct gtcaaagtta aatttccaat tgtatgtaaa tatttatcat gtatctcctt
      781 atagttatct cctagtgatt ccttccattt atttgttaat gtctgtggca ttatatgctc
      841 tatatttagc atattattat tgattaaatt ttctaaatca acaatttctt tattactata
      901 attctctaac ctctcaagta tatataactt attctttgac ttagtattat atatatcttt
      961 agtttcaaaa gcatttgaaa attcactgtc agtaggaaat ctttgagatg atttcttatt
     1021 tataaaaata tatcttaata tattagaata gttttcttta taatcactaa attttttaat
     1081 ttcccttcct aagttcataa atactttatt cagtgcattc ggaggtacat cacatactat
     1141 tcttcttaat ataaaacatt ctactaaatt aagggattct acaagtactt ttttatttat
     1201 tataccttca ttataatcat caaataattc taatataaac ggatatgata caaatatttc
     1261 aagtttgttt atccttttca aaactttgtt tacatcatta tcatcagtac atgaataaga
     1321 tatctctcta taatagttag aaaatttaag taaatctttt aataaaattt ctatatctat
     1381 accattatct gatacatacc ttttaaaact aaaatatact ttatttttat tcggtatact
     1441 tctctccttt atagtcaaat agtctcttat aaaactactt acgttgctac ttgtattttt
     1501 ttcaatttta taccaataat tattataaaa ttcctcttgt aacttagagc ttctacccat
     1561 taacacaaag tttcttactc tatcagcttc acttaaatct aagccagttg aatttagact
     1621 ttcaaagatt aattgagggt catcttcacc attttttaac tctatttcta ctatagttaa
     1681 tttctgtata gcatcaaaca aatcatctat tgatatatta tcttctttta ttctattata
     1741 aaaatataaa taatttgaaa ctatattaga atttctatct gtattcattt cttcttcaaa
     1801 tagttcatag tacgcttttc tatcttctgc tattggtttt aactttattt ttccatcttt
     1861 atctcttgag tgcttgttta taagataaca ctccttaatc tgttcttctg ttgtaataat
     1921 tccttttgat tccccaaact cttttattat ttgacacatg gcaatcaata atattgacac
     1981 agtagtcact ctttgttgtc catctattat taaatattct tttcccatac tatatataga
     2041 tacaactgac cctaagaaat gagttctaaa tccagaatct attatatcct ctaaatcatc
     2101 aaacaatctt ttacattgtt ctttcttcca gttataattt ctttgatata caggtactac
     2161 aaaacttttg tttgacccct ctaaataact aagaaattca atttctcctg ctttcatatt
     2221 aactccccct ataagttaaa taaattccat tcataactcc atgtatctat atattatatc
     2281 aaaattttga ctcataaatg tatatgaaag ccttattata taccttttta tacctgtttt
     2341 tattttatta aagtaaatac atgattattc tataaaatat acaaatactt aattttaaaa
     2401 attattattt aatcttcttc atgagtaatt catagttatt tatcaattaa aaagagatat
     2461 tgcataatta ttcaatacct ctctttgtat atttacatta tccatatcta gttatctatt
     2521 tcaacccaat attagactac ctactaataa atttattctc tacatcatta acaataattt
     2581 tgcactaaat aacatcatta gcctatctct gatgcatttt ctttaatttt atttatagtt
     2641 ttaatcaatt tatttctctc ttcaatatga ttttcaaata aatcaattat acttccaaca
     2701 gttctaaatg ggtcttccat caacactttg ttatcctcta taaatccatt cttgactaca
     2761 taatcgacta taagtttaac aaaatgtatt tgcttagtat taaagctctt attatttaaa
     2821 aactctgaaa aagcttcatt tgcagcttct ctatccaatc ccactagtct tctcactaat
     2881 tgacttactg gagtatctcc aaactccttc ttataatcat cacaattacc taactgtttt
     2941 aagagtattt cctccaacgt ttcaacatct tgctttgtta tctttttatt atttcttaat
     3001 ttaaatattg ctaattcatc tttatggcta tttaaataat actctacctt ctttttataa
     3061 ttcttaagat tgtttgcatt atatacagaa tcatttctat cttctgctac aatcatatct
     3121 tcaaaacttg tataatatat cttttgacta gacttttcaa gatacttaat aagctctctt
     3181 aatgatatac gtacttcttc tacctcaaat atatctgaat cttcccaaaa attctctgtc
     3241 attgcttttt caatagtata tttttgttgc tgaatctgtg gtatagtccc aagttttgaa
     3301 agtttttctg ctgtagccat tacatgcctt atacctttac ttgcattatt attttgtaag
     3361 tatgcaagtt gtatattata cataactaca tcaaatcttt tagccaattc atcatcacta
     3421 tatgaagtta tgagtggtga tatatattct cttatctcat tttgtgctac tgctccaata
     3481 ttgctccaaa cattttcatt tttatactta tgtacataac ttaaattcat cctaactata
     3541 taactatcct catttaaagc ttttacagaa gttataagtt cttctaacaa ttcttttcta
     3601 agttctgcat actcaccttc tatatattta atatcttgaa gttctttaac catacttacc
     3661 tttatattaa ataatttttc agtaagtgtt tctgctttat ttccctcaaa tcctttagga
     3721 ttaaatttaa aaaattcaaa attattacaa aaatcaaata ttaagaatct ctccttatcc
     3781 atatcaacac ctaataaatc tggacataat ctagttcctc tacctatcat ttgccaaaac
     3841 ttagtcttac ttctcacttt cttgaaaaaa acaagattta gtatttcagg tatgtcaatt
     3901 cctgtatcaa gcatgtctac tgaaactgct atttgaggta atttattctt atcagaaaaa
     3961 tcatctatta tagaatctac ataatttata ctatagtcaa ctgcttttac aaagttccca
     4021 ccatactttg gatacagctt attaaatctt tcaactattg ccctagcatg ctttgtattc
     4081 tttgcaaaaa ttatagtctt accaatcttt tcttctcctt ctattttcaa cccctctgtc
     4141 attagcttat ttaaaactaa atctattgtg tcagaattaa atagccactc atttactgca
     4201 ttattcccta tttcgtctcc aatactctca tcatcattaa atgtattttc atattcttcc
     4261 ttctcttcat cagaaagctc atcatactta attccatctt ccataatctt agtcttaaac
     4321 tcaatactag tataatctac taaatatcca tcctcgactg ccttattata ctcataagca
     4381 tatgtaggta ctccattttc catgtcaaaa actgaataag tatttttatc tatctcatcc
     4441 tttggtgtag cagtaagacc cattaagtaa gaatcaaaat aatcaaagat tgacttatac
     4501 tttttataaa tacttctatg cgattcatct attataatta aatcaaaatg accaggtgta
     4561 aacaatctct ttccatcctt agacttcgta tcatcaattg cattcatcat tgttggatat
     4621 gtagaaaata tcatcctagc atcttctggg ctgtctttgc tatccaataa attacataac
     4681 gccaagttag gtaatagctt agtaaaactt tttttagctt gctttaccaa tgcttttcta
     4741 tctgctagaa ataatatatt tttaatccaa ttatgtcttg aaagtacatc aactatagat
     4801 atagcaattc ttgtttttcc tgtaccagta gcacatacca aaagagtttt gcgttgctta
     4861 ttttcaaggg cctcacatac agcccgaata gcttcttttt gataatatct attggatatt
     4921 tcatcattta ttgttacaga acttaaagac ttcttactct ttcttctatc catcataagt
     4981 tgtaattctg attttttata aaatccataa acttttctat ctgaatagtc atcccaaata
     5041 tacatatcaa aaccattagt caaaaatatt actggtcttt gactatatct cttttccaaa
     5101 caatctgcat ataatttagc ttgttgttga ccaatttttg ggtctttgct cgttcttttc
     5161 gcctcaacta ctgctaatgg cttaccattt tcaccataaa gaacataatc tacatatcct
     5221 acaccacttt cattaggcat accttgtact tctatttcct cccctatatc ttttccaaat
     5281 tcccaacctg caagctttaa ttctatattt atatatcttt ttcttgtctc aaattcgctt
     5341 acttcgtcta tcttgaaatc atagtttaca gtattaactt ttctcttttc agttagtgac
     5401 tttcttaatt cctcattttc ttttattact tcttcaagct ttttatcttt tgaacttaat
     5461 ctctcgtaaa gatttttaag ttcgtctggt ctcgtccttt tctcttctcc atgaagtaat
     5521 aaatcttcat taaagtcttc tgctgtatat tcatctgaat aacaatagtc tatccaacta
     5581 acaaattgat gcaaattgtt aagtgaaagt attgcttctc ctctctctat gttcgcacta
     5641 gtatgtacag ataaattacc taacttaact atatatttta ataaaggtaa catatcatag
     5701 tctataagtt ctataaaact attattatga atcaacgtac tgagattatc ttgataaggc
     5761 aatactaaat cactatcatt agcatatagc cattttactg caagctctaa agctcttctt
     5821 gttatagttg cacaagtaga tggactaact agtatacttt tttctgcttc taagcaagca
     5881 gtattaaaac tattgaaatt tttcttcttt tgtaaaaaat caaaattacc caaggctttt
     5941 atcccccttt tgtcccttag ttaaaataaa tctctctaaa aactatttct tacagctgtt
     6001 tcttatacta acaaatactt cttcattttc tgtaaacaat tcgtacatat cttcaaactg
     6061 tggtatatca tatccatgag aatcatcatt tctcaatttt ctaagtgttt ctataactat
     6121 agaagaattt ttaggtaata aattgtgata ctcacaagct aaaaggtagt cataaaagtt
     6181 tttagatttt atgtttaaat caattgattt ataatacaac attgcttttg ttaattctag
     6241 aaatgatacc atatagcttt taaagccttc ttttatgtat ctagcccata actcaggcat
     6301 ttcttcctta tcatataaga ttttgaagtt ttgcaggtca tacaagcaag aaccaaaatc
     6361 cctatttact tcttctattg agttccaaga taatttgttt ctaattacca tacatgtccc
     6421 tccaatctaa gatatctatt acatcactgt tttcaaaata gaatttattt atataatcct
     6481 taaaatcttt agattcataa ataacttcac cttctaaaat agctttttta agtgctattg
     6541 tataattatc tgtaacaatt tttaggtcta catcaatgtc taatatattt cttagagctt
     6601 ctgtcttagt tgcaagttct gtaattggta cttttttgat gggaatccaa gctatgtcta
     6661 tatcactttc tgatgtatgt ttatctgttc cataactacc aaatattatg attgctagag
     6721 tattttcttt tggaaataca tttattattg agtttttaat ctcatttggt atatttctca
     6781 tttaatcacc tcttgtttat taggtaatat tgtttagtta aataattctc ctttgaaagc
     6841 tttttgcatt aatgaattaa agttatcttc taactctttt aagttggttt ctatttttaa
     6901 ttttaattta tccacttgct taacaaactc agaaaactga ttttgaaatt caattggagg
     6961 aaccattatt aaatattcac ctaacttact aactgataac tcaatttgat tcgttgaacc
     7021 attaacacat tttctatata ttgtgtcttg tatatttgta aattcaaata acgctgcaaa
     7081 ataatctgaa tttaagttat ttgaaatact tcttaaaact gtaacatgac tatctgctat
     7141 atattcagaa ttatcaattt tgttaaattt tacacatctt cctaatgtcc cagttccagt
     7201 agaatttatt aatatatcat tttgtttcaa aaatatatct tctatcttct cttttaagtt
     7261 ttcatcataa tatttacaat tttctacctt caaatttctc cagtatatac atttttgatt
     7321 tataactttt atatttgatt gctctacata tttaggagat acacctcttt ttaaatatat
     7381 agaaatctct ttaatcttct tcatatcaaa tttttttgta ttattaattg gctccccaaa
     7441 catctcaata aatcttgatt taactaattc atccaatgct tctatttgct cttttctttt
     7501 atttattaac tcttgagctt tatttaaaac ttctaccatt ttattttgta tacaaattgt
     7561 gaaatatggt atttttatat catttaactt gcttttattt aaagttgcac ccataactgc
     7621 tctatcagta ttattaaaat ttaaagttct caaagcataa tataaatatt cagatgttat
     7681 aagttcatta ttttttattt gaaagttagc aattgcttca tttgtaaaca tgtcttcttt
     7741 taatatggca actctaccta tagacaactt aaaactcatt acaacggtat ttttaggtac
     7801 taatttcata ttagcttttt caacagctaa atctgttatc tcttcttttg attttgatat
     7861 atatttttct ttcaaatcag aaattgataa ccatctatta ccacttcccc aataatcaga
     7921 agtatttcta gatggtgttt tgccaatatt tatatagcac aattcattta attttatata
     7981 ctccataccc tatacctcta acattctttc taattcttct aatccaacag ctatttcctt
     8041 ctctaactcc ctaactctac ctataatcac actaggagca tcataaacta cttcctcata
     8101 cactacctct ttatacttat taatagataa atcataccca ttctctctta tctcttccac
     8161 atcaacaaaa aagctttgtt ctgttctttt tctatctact tccccatcaa gattatcaaa
     8221 tctcttaata atatcctcta tatcactatc ctttatctca tttctcttat catccaaaga
     8281 ataaccatca gctttcatat catagaacca taccttatca gttccacctg tccctgtttt
     8341 agtaaatatc attatagctg tagatactcc tgcataaggc ttaaacactc cacttggcat
     8401 tgatattata gcttccaatt tatgattatc aactatttct tttcttatcg atttatgtcc
     8461 acctgttgaa ccaaacaata ctccatctgg cactatacat gcgcatcttc caccaatttt
     8521 taaaactctt aaaaaaagtg ccaagaataa taactctgtt ttctttgtct tagtcacttt
     8581 taataaatct gctgacactg cttcataatc taaacttccc ttaaaaggag gatttgctaa
     8641 caccaatgta aacttatctt tatctgtatt aacctccgat aaagaatcct tatactctat
     8701 attaggattg tcaacgccat gcaacatcat attcatagca cctattctaa gcatagttct
     8761 atccatatca aatccataga acataccatt attaaaatgc tccttcagcc cttgcactaa
     8821 gaaaagactt gaatgattat cccttaagta ttgttgtgat gatactaaaa aacctgctga
     8881 acctgctgcc gggtctacta taatatcttc tggtgttggt ttaataagtc ttactatcat
     8941 atctattatg tgtcttggag tcctaaattg accattagta cctgctgtag ccactttgga
     9001 taataaatac tcatacaaat ctccctttgt atctctatcc ttacttaatt ctaaattact
     9061 aataccatca actattttag ataacataag aggcgtagga attttaaata ttgcatctcc
     9121 catgtacttt gcataagctg agtctttatt tccatgtaat ttttttatga aaggaaacac
     9181 ttcattttgt actatttcat acattttttc tgaatttcct tcattagaaa acttactcca
     9241 tcttaaatgt tgatgttcat catcaaatat cctttcaaaa gaaactccca aaagagttgc
     9301 ttctgcttca ttttttgttt ctacttcatc caatccttta ataaataaaa gatatgtaaa
     9361 ttgttctata acttctaatg gattagttaa tccaccagtc caaaatgtct cccaaatctt
     9421 gtctactttg ctccttaatt ctcctgtaat catatatgac tttccctcca gtgttcataa
     9481 aataatttaa atatatattt tagtcgtacg ccttaataat tagtgtctta tattaaattc
     9541 aaaattttac tacacagtta ttaaattatt tttcctattt acatgtttta gttaaaaaac
     9601 taatgcaata ctcttatttt atgattatta tacatcccat acaaaattat tttatcatat
     9661 atccagcata gaagtcatct acctggataa ctcttaccat attataactt tttaatatta
     9721 atttaaactt agaaagaaat tttttattta attatatcat cttccttatt tcaaatttct
     9781 caattacaat ctcaatcaaa aaataaaata agatgctgtc tcaaaataga tataatcttc
     9841 ttttttattt tcggaatgat gaaaatatat ttaatttcat acaaaaaaga gtgcctcctg
     9901 aactaaaaag ttcattttga gacacccctt aatttaatct tacttattct ctccctttat
     9961 tattaacact tcactatctc tattgctttt cctacacatc cacaaacttc catcctactc
    10021 ttaacaaaat ctttaactga ctctaaacct tttttaccat atttcttagg gtcaattacc
    10081 tctgggtcag aattaagcac atccttaacc ccattgctat aagcaatcct taattcagtt
    10141 gcataattaa ccttgcaaat ccctcttttt atagattcct taataacttc atctggtatt
    10201 ccagacccac catgtaatac taatggaact gatacaacat ctctaatttc agataatcta
    10261 tctaaatcaa gctttggctc tcccttatat aatccatgtg ccgtacctat tgctactgcc
    10321 aaagatgata cccctgttct ctctacaaac tcttttgctt ctaatgggtc tgtatatgct
    10381 cccccatcac caccatctaa atcgtcttcc ttacctccaa ctttacctaa ttcagcttca
    10441 acaggtatat ctgaaggcat acatgcatct acaacagatt tagatattga aatattatct
    10501 tcgtaacttt catgagaacc atcaatcata atagaagtat atccaactct aagtgcctgc
    10561 atagctagtc caaagcttga accatggtct aagtgcattg ctactggaac acttgccttc
    10621 tctgatgcta cctttacatt cgctaaataa taatctagcc ctgcatactt tattgttgaa
    10681 ggtgttgttt gcaatattac tggtgattta agttcttcag cagcctctat tactgccata
    10741 accatctcca tattttcaac attaaatgca cctactgcat aatgtccttc ttgtgcatct
    10801 aataatattt gtttagttgt cactaatgcc ataatttctc ctcctatatg tttatactga
    10861 tttgatttct tcgttattta attttgctac tatttctcta cttgttttgc tatttaataa
    10921 ctcatttaaa aattcttcat tcattaattt tgaagatagt tgagataatg tttttaagtg
    10981 taaattatca ccattatctg tagctgctat cataaatata atatgtgcta agcttccatc
    11041 ttctgattca aagtcaaacc cttcttttga tacacctact gctacacgtg gtacctttac
    11101 tgcatctgtt tttgcatgtg gtatcgctat ccccatacca aaacctgtag atgatatttc
    11161 ttctctttta aggatttctt ctttaaactt ctttttatct aaaagaacac tagatttatc
    11221 aagtattcca atcatttcat caattacatc tgttttattt tttcccttaa catctaaaat
    11281 tactgtttct tcgctatata aagagatatt attagatacg ttttccgatt ctgcactttc
    11341 atttttatgt tcatcttttg ttacatttgt attttctata gtatttttct catttactac
    11401 tttaggtgta accatcatat aaagtacagc acctactaat gaacctacaa atatacaccc
    11461 tacccataaa agtggcttat tgactaatcc tagaaccaaa aaccctccat gtggagctgg
    11521 tacttctatt ttcattatat aagttaaaat tgatgatatt gatgatgcaa tcataagtac
    11581 tggcaacact tttaatggat tttttgctgc aaaaggtatt gccccctctg ttatatgagt
    11641 actacctagt atataattga ttaatcctgc tgctctatct tcttctgtaa atctattttt
    11701 aaataaagta gatgctattg ctattaccaa tggtggtgtt atacaagctg cagatacacc
    11761 tgccataaat aaataatttc cctgtcctag aagcatagtt ccagttacat atgctgcttt
    11821 atttactgga cctcccatat caaatgctga catacatcct attactattc ccaaaattat
    11881 agggcttgaa ttttgtaacc ccgatagaaa gttcatcata gcattgttaa gtgctgaaca
    11941 tggcccacca agtaacaaca ttgctacacc cacaagacct acgctgacaa taggaattat
    12001 aaaaatcgat ttaagacctt ctaattgtcg tggtaatttt cttaacaaat gtacaaatag
    12061 tttaactaag taacctgcta aaaatcctga tatgattcca cctaaaaacc ccgaacctgt
    12121 atcaaatgat atcattcctc caacaagtcc tgctaacata ccagctcttc ccgatataga
    12181 ttgagctata aatgctgcca atacaggaac catcaagttc atagaatatc caccaacaga
    12241 ttttaatgta gctgctattt gattatattg ttctgaattt gggtcaaatg aataaatccc
    12301 ccataaaaat gatatagcag taagtactcc acctgctact actaaaggta acatgtgaga
    12361 tacaccattc attaagtctt tatacaactt ttgaccaaac gataaatttc cttcttctgc
    12421 aattttactg cttgataagt ttccttttct aatagctact tttccatcaa tacatctttg
    12481 tataagttca ttagctttat gtataccttc tttgacaggt acttctatta ctggctttcc
    12541 attgaatctg tccatatcaa catttttatc acaagcaact attattgcat ctgccatttc
    12601 gatatctttt gctgttaact tattttctac acctatagct ccattcgttt caacttttat
    12661 gtcacaccct aattccctgg ctgaattttt taatgcttct tctgccataa aagtatgagc
    12721 tattccagtt ggacaacctg ttacccctag aatcattttc tttcccattt ttataccacc
    12781 tttctgactc ttatattttt tatataatct tccacattct ccaaattacc tataccatta
    12841 ctttcagcca cacttgctcc tgttgctgct gaccttttta atgcttcttc aatatgttct
    12901 ggattttcca accatatact taaaaaagca gctaatgcag aatcaccagc acatgcagag
    12961 ctgactactg taactggttg agcactagca taataaattg attttccatt ataaaaataa
    13021 gaaccttttt ctcctaaagt tagcaatata ttttgagcac cttttaaatg tagtaattta
    13081 agtacatcct taatatcttc ttcatctctt acaataattc caaatatatc tttgatttct
    13141 tcatcatttg gttttatcaa atatggatta tactgtagta actcctttaa ttttggtgaa
    13201 cttatatcga gtattgtctt tatactttta tttttacaaa ttttaaatat atcctcatag
    13261 taattatcat caataccagg aggtagactt ccacttattg ataagtaact tatatctgct
    13321 aatgactgta tcttatctag catatctagt ttttgttttt catttacata tgacccagag
    13381 tttacaaatt taaattcatc acttccatca tttaaaaata tatttatacg agttgtatct
    13441 tctacccaag taggcaatac gtcaaatcct ctcttttcgc tttcttcaac tatatatttt
    13501 cctgaaaatc ctccaaaaaa tcctaatatc ttagattcta taccataatg acctagtaca
    13561 aaagttacat ttaatccctt tccattagga gaatatacag catttgatgt acgatttact
    13621 aattttctct ttatcccatt agtagatata ttcatatcta ttgcaggatt tgtagttaat
    13681 gtatatatca ttgtcttccc ccttgcttgt gctgtgtatg ttttcattat aataaaaaag
    13741 tctatgaaaa tcttttcaca aatagaaaat cgattttcat agacttttca atttaatttt
    13801 cagaattatt atatctgacc atataatcta taattatcct agatataata tacaagggca
    13861 atctggaaga tacttcatat tcagaaattg atatatgttc atgtttgtat cctacaatat
    13921 ataaatccga atactttata agttttgcac tttcactaca acagcaagtt ataaccttag
    13981 ctccaactaa attggcattc tgtgctgatt ctacaagctc tatggtattt ccattcaatg
    14041 aaaatataat tataagttcg tcttttttta ttgatttaga ttttattttc attatgttgg
    14101 ggtcatttat aaaaacaaca ttaaaatcaa gtaattgcaa tttaagacta aactcttttg
    14161 ctacatattc agtaagccct cttgctaaaa tatatatctt actagattta tttatagttt
    14221 ttattatatt gattagtgta gttaaagata tttgttctat aactctctgt gcttctataa
    14281 aggatttatt tattatatcc cccatatttt gtatatcatc attttcatct tttactgtta
    14341 atctatacct aagttcattg aatccattta aaccacattt tctaattgct cttgaaacgg
    14401 ttgctggaga tgaatatgtt tcatgagcaa tctctactat agataattct ggtaatattg
    14461 attcattttc atttataaat ttaattattt caagttccgt cttcgttaga ttttcagaat
    14521 tacttttgtt tagctcaatt atcatatgtt atctcccctt atataagttt tatttgtcta
    14581 aatttatcga gtaatataat atattacctc ttaactactt atttttatat acaattaaga
    14641 tttagtcaat gtctatatat gtaaatttat aatataataa ttttttgctt aaagtttatt
    14701 atgttatctt atagcatttt aaaataaaaa agaattgtac ctctaccaaa atttggatga
    14761 agtacaatag atattcctca tactgatatg tttatgatta aaagtataaa tataaatttc
    14821 gctatgaatc atataattaa taaatcattt tatttacaat tataaccccc cttataaaac
    14881 tatattaata atatatacag tatttataag gggggttgtt tatatttaaa aactatttac
    14941 ttttctctta attcttctcc attagttctt attacatttt gataccaata aaaactatct
    15001 tttttataac gttctttact tccatttcca ttgttatcta tatccacata tataaatcca
    15061 tatctttttt ccatttcttg agatgaacaa cttactatat ctattggcga ccacatacaa
    15121 taagctaata tttcggttcc atcagctata gcttcaccta tttgttctat atgttttcct
    15181 aaataatcta ttctgtaact atcatgaact tttccatctt caaatttatc atgcattcca
    15241 aatccatttt cagctatcat tattggcttt tggtatctat cataatattg agataaagta
    15301 ttgtataatc cttttgggtc aattgcccac ccccaaggat ttgcttttaa atatggattc
    15361 tttatattgt cagttggatt catggtgttt ttacttgctg atactgttgt agaataatag
    15421 tatgataaag ctagaaaatc catagtattt tcttttaaga ttttatcatc accttcctcc
    15481 ataactatat ttatgttatt ttctttgaaa aatctcttaa tatagactgg atactctccc
    15541 ctaaaactta catcagtgaa aaaatattgt agcctatttc tttttaaagc aaatactaca
    15601 ttttcagggt cacaatcttc tggataagat gtacaatctg ccatcatcat acctatcatc
    15661 atatctgaat ttatttcttt tccaagcttc ttagtcaaag ctgtagctat gaattgatga
    15721 tgaatagctt gatatctagt ctcttctaca ttatcaactt ggtctacaca aaatgcaact
    15781 gaattaaatg attcataatg tattaaattt atttgattta taactatcca atatttaact
    15841 ttatttttat atctatttag tattaattct gcatatctaa caaagaaatc taccactttt
    15901 ctattagacc atcctttata ttttaatact aaattcaaag gagtctcata gtgaagtagt
    15961 gttacaatag gttccatacc attatctatt acttcattta taagattatc ataaaacttt
    16021 aatccctctt cattaggctc taaatcatct ccatttggaa atattctagc ccaatttatt
    16081 gaaaatctaa aacactttaa ccctagctct ttcaaaagct ttaaatcttc tttgtatgta
    16141 tgataaaagt ctattccact acgcttagga taatatccat tattatcttt catagcaaac
    16201 tttattcttt ctatagtcat atcagactca taaggtttag atatatctct atctttatca
    16261 tacatatgta catcagctaa acaccaacct ttgccacctt cattccatgc accttctaat
    16321 tgattagctg ctacagctcc tccccataaa aaacctttag gaaacttacc caatttaatc
    16381 actccttatt ttatttattt aaatcattta ctattattta ccttatattc tatagacttt
    16441 actattccta ctaaagacat agttatattt aactcgctga atatggtcat aagagtatct
    16501 tgagcatgtg caaataacag cgaatgtgct cttttctctc caccagcttc cccttgtata
    16561 atcttggttt gagctctatg tgcttctgtc ataaaccctt ttgctttagc tagttttaaa
    16621 tcagcatttc tataatcaaa cttttccaaa tcattaagag catgtttaat ttctgttcta
    16681 gcctcacctg cacgaagtat tatttgcatt gctacttcta ctaattcttc atctattata
    16741 tgttcttcaa ttgtcatatt tataatttcc tttcaatttc taaatttaaa cctcttgact
    16801 ctctaatttt atctcttctt tctcataagc tttaaaaaat ggataccata tcataaatag
    16861 caatactata tttattgcta aaacaataag acctctaaca tcttgattta ccataaaagt
    16921 agatatacca aaaggaacat accataaatt aaacacctta cttggaattg taacaaatcc
    16981 catactcatt gataaataag tagttattga tattactact gttcctatcc acataggtaa
    17041 cattaaaaat gggttccatg ccactggagc tccaaatatt actggttcat ttatattcat
    17101 tatagaaggg acaatacatg ctcttcctat tgcttttatt cttccaactc tggataaaca
    17161 catcattata actaatggta atgtagcacc caaccctcct aaagttatcc agcctgtttg
    17221 aataacttcg taaacatata tattagttgc tactcctcct gatgctactg ctgctgcatt
    17281 agctgcaatt gcaccaagtt gaattgaata agttatagga gatagtaccc acccacttat
    17341 tcctaaagaa tatataaata ctactaagaa gtttaagagt acaaatccag gtaatgtctg
    17401 tgctatatct gatactggtt taaatactct aactattatt tcaaacatgt caaagttgag
    17461 tccataaatt aatccccagc ctacaaacat tatcactgtt ataggtaaca tattatcaaa
    17521 ccacttgctt acaaaatccg gaagtgctga atcttctccg aaaaatgaaa aagaaccaaa
    17581 taaagacatt attaatccag agaataatcc tgcaactagc gatacaagca ttccttctcc
    17641 tccaaacctg ttgaagttaa atatagctcc ttcattcgta atctctggac gaatcatcat
    17701 taacattaat ccagcacctg caagaccagc tactacactc atactttcaa atttcttatt
    17761 cttcattgtt tccataggca ataaaaatgc tataaataat cctaaaagtc caaatgtata
    17821 ttgatttatt ggacttaaat caggtaaaaa gcttatgtaa ttcttcaaga cagaaactat
    17881 agttactaat gagcctacaa ataccattgg caaaactttc attatagttg attgaactga
    17941 ttgtacccat atattcttag tcatattatt tacaacagga gtaaattttt cacttaaaaa
    18001 atctataaat ttattcataa acactctccc cataaatatt ttatttgttt gatattagtt
    18061 ctagtgcttc ttccaaagcc tttgcaccat ctagtcttcc atatgcagtt ttagatatac
    18121 atgctactgg aacgttatat tcattagcct tttgttgaat ttcttccttc atataatcta
    18181 aatgtggtcc tagtaataaa caatctattt ctgataaata cttatctatc tctgactcac
    18241 ttctcgcttt tatatctgct tctatacctt ttttctttgc actctttctc atactagttg
    18301 ccataaaacc tgtacttgct cctgaaccac acgccaaaag cactcttata tttttcataa
    18361 taatacctcc aatattttta tccattaatt tatttcttaa tgaacttttt attttattat
    18421 gtttatattt tacttcccaa ataagttcat ttcacataaa tatatttcct aataatagga
    18481 aatcatttac tcttaattag acctattaat atagtataat gtatcttata atacgtacat
    18541 tcaattatat aattatcata tcatacagat tttattttaa aactatgcga gggaatctaa
    18601 tgaaaacatc taaaaaagag ctatttttaa atttcataaa aacgcaacat gattggatag
    18661 attcatccac attagctaat taccttaatg tatcaacacg ttcgattaga aaatatgtaa
    18721 acgagattaa ttctaatggt gcatttatat tatcttctaa aaagggctat aaagtaaatc
    18781 tcaatactaa ttgtcaaact aagattgata gttcagagaa tatatcacct gataacagat
    18841 taaatttaat tttaaaagaa cttatagtta atagtaatgg aattaatata tttgatttaa
    18901 gtgaagaatt atttgttagc ccagctacta ttgaagggga tatagtcaaa gctaataaat
    18961 ttataggaag ttataattta aagataaagc aatcgaaatt tttattgaaa ctagttggaa
    19021 atgagtctgc aaagcgcaag ttaatgagct ctataatatt taaagaaacg ggaagtgatt
    19081 ttttaagctt atttgatgta caaaaaatat atcaagagta taatttgact aaactaaaag
    19141 aaaatataat atatatatta aaaaaataca atttatttat aaatgagtat gctataaaca
    19201 acatacttct tcatttaatg ataactatag acagaataaa aaagaataat tacatagact
    19261 ctgttgaagt agtaaactac atagacaata acgtagatat aaatattgca aaagacatat
    19321 caaatttttt agaatctgaa tacaatataa ctttaactag tgcggaatta tactatttag
    19381 tatttcagtt aacaaacaaa actacagtct taaactacaa tcaaatggat actaaatcat
    19441 tgtcaaatta tatagatgaa cactttgtta aattaactaa aaaaataatt aaaaatgtat
    19501 atgacttata tcttatagat ttatctgatg aggaatttgt tgtaaaattt actcttcatg
    19561 taaaaaattt aatcagtaga gccaaaaata atcaagtttt aagaaatcaa atacctcaaa
    19621 agttaaaaga ttcctaccct ttgatatatg atatatctgt ttatatttgc aatcagattc
    19681 aaactttaga aaatgtagat atagacgaag atgaaatatc ttatatatct cttcatgtag
    19741 gttcattctt tgatagacaa aaattattag aagataaagt tctatgtgca ttaattactc
    19801 caaattacta tgatttacaa ttcaaaatag taagagacct tgagaagaga tttaatgaat
    19861 ccatagaaat tattcagatt ttttcagata ctcataatct tgattttgac aataaggtag
    19921 acatggttat tactacttta ccaattaacg atagatgccc tatacctttt gtttatgtaa
    19981 atccttacct aaatagaaag gattatgata atattcaaag taaatttatt cagattaaag
    20041 atagaaaaaa tatacttact gtacaaaatc atttagaaat gtactttagt gaatctttgt
    20101 ttatgaaaaa tatatattta gatagtgcta aagattatat aagatttatg ggaaatattc
    20161 tgtacgaaaa taaatatgtc aaacccaatt atatagatga tgttcttata agagaaaaaa
    20221 tgtcttcaac tgcatttaat aacaatgtag caattcctca tagcatgaaa atggatgctt
    20281 taaaaaccgg agtatgttta attgttaacg ataaaccagt taaatggggt gaggaaaaag
    20341 ttcaaattat agctatgata cctataaatg aaaaagaaaa agaaaaattc aattacatat
    20401 ttgaaagctt tattgaaata ttatctgaat ggaataatgt taaagaactt actaaggcag
    20461 ataactatag tagttttatg aatagaatag cttatcttat tcaaaatatc taaatccatt
    20521 aatttatttc caatatatgt ttcttaagtt attaacataa aatagataag ctgtcacact
    20581 aaaaagtcta aattaatatg tgacagcttt ttacctaata taccttgttt taatactaca
    20641 tattttttga ctgttgtata ctagttataa gattgaatat ttgtgttgtt gtagctgtca
    20701 tattctcact agcagttgct ttatccattg cttcttctat tgtcatagct ctatttacta
    20761 tagaaaaata agcatctata ccctcttcat tacatttaac tgcatcttct gttgtggctc
    20821 cagccaaacc tattactttt accccatatt tttttgccag ttttgcaact cctattggag
    20881 ccttgcccat agcagtttga ttgtccaatc taccttcacc agttatgaca aaatctgcat
    20941 cttttaactc ttcctctaat ttaatttctt ctaatattat ttttattcca gattctaatt
    21001 ttgaatctaa aaatgctaaa aaagcaaaac ctaatcctcc tgctgctcct gctccttcta
    21061 cattagctac atctttactt aagtaatttt taactacttt ggaaaaattt ctaagcccat
    21121 tatctaactt ctcaattatt tctgatgttg cacctttttg agacccgtat atgtatgcag
    21181 ctccattttt cccaaacagc ggattattta catcacaggc tattttaaac ttacattcat
    21241 ctaattcctt taatctattt tctattttta tatgtcttat ttcatttaaa acttttccac
    21301 ctaatcctac taacttgtta tttttatcat aaaactcaaa ccctaaggct tgaagcatac
    21361 ctatcccagc atcattagta gcacttccac ctatcccaac tataaaattt ctatagcctt
    21421 tatttattgc ttcctttatt atttccccaa caccataagt tgtagtgtat aaaggatttc
    21481 taagttcagt cggaaccaat gttaatcctg atgcttgagc tatttctatt attgctgtac
    21541 ttgtattttt taataatcca tatgttgcat ccactttttt agctattggt ccagtaactt
    21601 ctatagtctc ttttttacca ttcatccctt gaactaaagc atctactgtc ccttcaccac
    21661 catcagcaag tggcaatatc tttacttgcg catcttcttt aaccttcaat atcccctttt
    21721 ttattgcatt tcctgcctct attgaagata agcttccttt caaagaatct atagaaatta
    21781 atattttcat acttatctac tcctttttat tactccttat tttaaacata taattttata
    21841 agcttaattt atcttttatt ttatactcta catctctctt tgtatacaac ttataccatt
    21901 aatattcttt tatcttataa aaagggagtg tctcaaaata aatttcttag ttcatgagac
    21961 actcttttgt ataaaatcaa atatatgttt attatttcaa caatgataag gaggattatc
    22021 tctattttaa gataattcct ttttagcact atctaatcat ttttagcact atttaaccag
    22081 tatagtttta acttggttca ttaagcactc tacatttaca gagttattat ctattgatat
    22141 agattctaat tctttttcat ttaggttaat ccatttagct gttttaatat ttttgttaaa
    22201 tactatgtta gctgattttg tttcttcttt agaattataa aatctaacta ctaatgcctc
    22261 ttctttttca gcctttttta caacacttaa cacaaggtcc ttattagttt ccttaagtaa
    22321 actatatgaa taatctactt ctatttctga ttgattcaat ttcatagcat tatatggcat
    22381 tttattataa gtttgtattt ttgttaaata ctctttagcc atatttgcaa catttgcttc
    22441 tagtgtagat ttttcatgag taactattgc taagtctatt tttatatctc ctatcatttg
    22501 agaatctgga gtaggcaact ttattcctga tggacgacct gggcgtcttg ccatttcttc
    22561 tttacctagg aatcctacac ttctaaataa tgttatagct attgtgtcaa atttttcccc
    22621 tactatttca aactctcttg tactgtttgt aagaacagct actccatgct ctttgtcaga
    22681 caatccaacg aatgttagca ttggatatat agaatctggt ctttcactcc agtcttcctg
    22741 ttgccaaact tccatagcat catcatacac acttctcttt atatgtccaa actgattatc
    22801 agacacagaa aactttgaag ctatatttgt tggtatataa gttctaaccc tacaatctct
    22861 tgcttggtta tttatgttga atactacatc tattatagct ttatcatttg gaacatttat
    22921 aactatatca aagtcaataa atgaatctgc tctattattt tttctagctt gtatatcttt
    22981 tggtactttt aatctataat ttatctttat ctcattatta aatctatttt tctttatagt
    23041 ggaatttgct actatattat cgttaaatat taatttctca tttggaagtg gtgaaaaatc
    23101 atactcatct ccttcatctc ctccattttc catcaataat acattttcaa aagttttatt
    23161 taatttttta tcaaatatct ttaatgtccc gttttcatta acttgaatag tataaaagtc
    23221 attatctacc atgtcacata cttcataagc tttttctaac atatgttcaa cttcttttat
    23281 catatacgcc ttatacccca ttgcaggtat tttatcttta aagtttattg tatataccat
    23341 aaatgaatca tagtttccat aatgaacaat ttgacggtct attaatcctg catctattac
    23401 ttctttatgt accacttcaa aatccaactt attattatct ttatcaaata tttcaaatgc
    23461 tttcatttta gttataactt gggcgctcac tatttcattc ctatcgtaag gcattaagtt
    23521 aaatgctatt aacttatcta aagccatttc acaactcatt gcatcaacta ttcttctttt
    23581 gtaaaatgtt attaattggt ctactttttc ctcagctaag aaaaatctat tcattatttc
    23641 ctggtgaact ttatctgaac aacaacatcc aatgctgtca tgagcatgat ttttcattat
    23701 ttctttccaa attaactcta ttaatccatg atgatattca aatcctagag aatatgcaat
    23761 agaagctagt ggctccaata tgttagttat ctttgattct attctagcat ttgctgattt
    23821 tatatccatt cttgttgaga atatacttct atgaaccctc atatatttcc catctaaaaa
    23881 ttcccctttt attgtatcta tatcctcatt tttttctagc tctttaaata tgttttcata
    23941 tctacttaag aaaaattttc tgtctggata acattctttt aatttatata taacttcaaa
    24001 tatattcttt tgaattggca tttggtcatg tccatttgga agtattatat ggtcagtagt
    24061 tgcaccctta tcaagaaccg gtaaatattt atccattctt gttcttagct catcttcatt
    24121 cattggtaga tattttccta ttgcataacc taatggaagt atttgaacca aaacagaaga
    24181 cccatcatca ctcttccaat taaactctgt tttattagtt ccttttcttt cagaacatcc
    24241 tctccaaaac attgaatatt ttatatcaaa cccatttagt atttgtggca tttgagcact
    24301 ttgaccaaat gaatctggta aatatcctat tttcatatat tctccaaatt catcacaatc
    24361 ttttattcca tatagtaaat ttctaagtat ggattctcca ccaactacca tttcatcagt
    24421 ttgagtatac caaggaccta ttattagcct tccttcttga actaattttt ttattctctc
    24481 cttacattca ggcttaactg ctaaataatc ttctaatata gcagtttgac catccatcac
    24541 ataatatgga tagttatgat tattttctaa catatccatt atttcttcca tattatttac
    24601 aagtaatatt ctagattcct cagttgagaa gtaccattct ctatcccaat gcatatgagg
    24661 aactatgtgt acattttttt tcattgctta tcctccaaaa cttatctgat aatattttct
    24721 gtctaattaa aaatccaata aaaatactac tgagtttaca aaatttttta atatttattt
    24781 tatcactata attaaacctt tgatttaaat atctaataac gtatctttta atgtatcttt
    24841 ctttgatttt tttgtttcct agttattatt aataatattg ctgatattat agctccaagt
    24901 actgccgcaa agaaccatat tgatgctata ataaatcctg acatacttcc ctctaaaagc
    24961 gccaatgaga atatccccgc tccaggcact tgaagaccta tattgaagaa tgctactata
    25021 gacccagtta tggctgaacc agctaccaat gatggtataa cacggattgg gtcctctatc
    25081 ataaatggta ttgcaccttc agttattcca gccaatccta atatccaagt ttgctttcct
    25141 atttcctttt ctgattctga aaataaatct ctgcctaaaa cagtagctat tgtaactcca
    25201 aaagctgaaa ccatcttaac tgatgcaaat attgcataag gtataaaatt tccacttgct
    25261 attgctccta ttgcaaatgt ataggctgct ttatttattg gtccacctaa gtcaaatgaa
    25321 accatagctc ctattattgc acctagtagt attgcattta ctccagtcat cccatttaac
    25381 caatttataa gaccattgtt aagtgctgct acaggctttc ctactacaaa taacatcaat
    25441 aaacctacta ccaaagaacc aactactgga tatacccaaa aacttacgaa tccagctaat
    25501 gtgcctttgg ctggtatatt cttctttaaa aattttacta tataaccagc aagtaaacct
    25561 cctaacatac ctccaagaaa tccactacct attaaatcag ctgctagacc tgctgcaaat
    25621 cctggtccaa gacctggttt ttcagctagt gagtaagaca tatatgctga taatactgga
    25681 accatcaatg tacctaacat acctccagaa agctttctta attgccataa ccaagaacct
    25741 tcttgattgt atagttcttg tagaccaaat gcttgagatg ctaatactgc aaatgcaagt
    25801 atcatacctc ctgccacaat aagtggtatt atatgagata ctcctgtaag tattgctgat
    25861 tttacatctt ttttccatga tgatttttca caatatgata tatcagatga tttttctata
    25921 taatcacctt taccttgttt ttgtgctact tctaaagctt cgtatattat ttcttttgct
    25981 ctttttagtg gagctgctac tgacgttttt atgtttggca aatgttcaaa tctttctgta
    26041 tttttaggtg ctacatctgt tgcatatatt attgcatctg ccctttttat tatatctggc
    26101 gtatgagggt ccaccatacc attagctcct tgcttttcta catacacatc cacacctatt
    26161 tctttaccag ctttaattaa tgcttctgct gacatatatg tatgtgctat accggcaggg
    26221 catgctgtta ttgctaatat aatttttttt gattcatttt gaatcctaat ctcttctttt
    26281 atttgttctt tcttttcttt tatatctaag ttattaaata actctttctt agtttttgaa
    26341 tttaacaaca tattcatata atcttcattt gacagtctag taactaactc agaaagtatt
    26401 tgtatgtgtg tatttccacc ttcactttct ggtactgcta ttaaaaatat tagctctact
    26461 ttattatttg ggtctacgct ttcccaattt tctattggtg tctctaaggt catagctgca
    26521 aatgcagctt ctttaactga cttagatttt ccatgaggaa ttgcaagccc tctttcaaaa
    26581 cctgttggag ataaagtttc tctgtacatt acagcttcta aatattcctc ataagatgtt
    26641 aacttgcctt catcatatag ctttcttgct aagaacttta ttgcgtcttc ctttgttttt
    26701 attttttgtt ttaaaaaaac aaggttttca tttgtgattt tcttaaattc caaaattacg
    26761 ccctccttaa atcatttaat gctatgaata tagcataaaa taaattatga aaatgttttt
    26821 ttgaaattat acacacaatt tcttcttaaa aaataaagat ttgtgtattt atacacaaat
    26881 ctctatctta accaatattg ctctattatt gctctcataa tcatcataat tggaacctta
    26941 tctgatatat tgtacccatt tacttgttta attgttgaat agcaatataa acttatatct
    27001 gcaatttgtt gcaattgatt ttcacatata tgtgttaaag ctactacctt acaaccctta
    27061 ttaactgctg tctttgctgc ttctataact tctttcgttt ctccacttaa actaagtgca
    27121 aaaaacatat cttgttcacc taattttcct atatcatgaa gtacttcatg tctttgacta
    27181 tagtaagtta tattatcatg cccacctcta atttctttta ctaatatctc acataaagga
    27241 atgttttgac ctattccata aactataata ctcctagatt tttgaattat atctgctact
    27301 ttatttattt tttcataatc tattagctct aatgtctttt ttatatctga ttcaaaacca
    27361 ttttttggct ctttatcctc attttttaac tcttctttta aattattttt tagttgagag
    27421 aaaccatcat actccatttt tttacatagc ctaactatgg tatttgggac agtaaaaaat
    27481 ttatctgcta aagattgtat tgataatttt actatttctt ctttgttttt tctcatatat
    27541 tcaattatca tgtcttctgt atctgtaaat ttatactcat atttttttgc tctttcataa
    27601 aattccatag tttctcctcc atctatttta ttattcataa gtaaaattca aaaatattag
    27661 ctaaaatttt cgttaatata attataattt aatattttta atttattaat gcatactact
    27721 ctttttatta tacataaaaa atgtgtactt cctatattga tagagatgat tcaagttttt
    27781 attttgatac atctctatca atattttttt agaaaataca catttttttc aaatacttat
    27841 ttttaattaa aactttaggt aatctattct ttatatataa ttattagact tgaaaatatc
    27901 tttaaaatca accaattcaa tattatttct ttttaaccac attttaactt ccttatcaca
    27961 tagcattgca tgttcatata ctctctcttg aataagagaa gatgatttta taagactata
    28021 atctataaat cctggatgag taaccatcaa tgttatatct ttatctaaat ttatatattt
    28081 ttcttcaaat aatttaacaa aagatttatt taacttgtaa aaatcattct gaggatattt
    28141 acttggcatg ctaatacatg aatccaaata atagggacaa cttggtatac cgtattcttt
    28201 tgcagcatca cacattgctg atattgatac atcttcttct ataccatgta tatcaatata
    28261 ctcaggcaat ttactcatat acaacttgta agtttcaatt tgagcacaaa cttcttcata
    28321 tacttcttca taattaaaaa cctctttacc tgtcagtagt tgcgttcttc ttattttgct
    28381 agaaacaaat tttccacctt catctaacaa attctttatc ttaatatgtt ctttgcaaca
    28441 aggttctcct attactaaat taacatgtaa accaaaacat acatcatcat tttttaccat
    28501 ttctatagct tgtcttgaaa aaggcatatt caccatgatt ccagcacttg taacaatacc
    28561 gttcttataa gaagatatta tccctagtgt aacaccttct gaatatccta tatcatctgc
    28621 cctaactact aatttcttca ttttatgcat ctgcctgctt tctcaactca tcaagttgca
    28681 tttccatctc atcctttact ttaacaacat ccgttcctat gatgatttga actgatgtat
    28741 tactcaattt aattacccct ttggctcctg tttttttaat attatcatca ttcaaaatag
    28801 tggcatcctt cacttctaaa cgtaatctcg tcatacaatt attaagagtc actatatttt
    28861 cataaccacc acaattttta attatctgtt tagccatata tgaaaaatta ccatgagaaa
    28921 gttttaaatt cttttctttt tcactaactt tatcacctat ctctatctca acttcacgtc
    28981 ctggtgtttg cacattatct tttattatta tatatttaaa tgttacataa tataatacaa
    29041 agaatacaac tcctacaggt ataactaacc acgctttatc tccataacta aaacttaaga
    29101 catagtcaat tatacttgct ccattaaatg aacccaagcg agcacccaat aaatatagta
    29161 caactccacc tagaccagaa taaaaagaat gaattatata taactttgga gatgcaaata
    29221 aaaacgaaaa ttctattggt tcagttactg ttgccataaa agaagtcaat gctccagcgg
    29281 tcattgcacc ttttacttct tgcttctttt tatcttgtgc tgttttatat atagcataag
    29341 aaatagcagg tattgaaaac atcataatta tgtaaaatcc tgtccagaag aatcctgcat
    29401 ttgggtctcc tgccataaat ctaggaattt ctcctcttat aacttctcct gttgatgttg
    29461 tatattcacc catttcaaat aatatgtaag catttaaaac ttgatgtaac ccagttggta
    29521 ttagtgcacg gttacagaat aaaaacaaga atattcctat tgcaccagct cctcccaacc
    29581 aaaaaccaaa ttgattcatt gcagcttgtg gatatatcca taataaacta aatatatatg
    29641 ataaaattaa agcaaccact ggtgatagtg taattacaca tttttctcct gcaaagaagc
    29701 ttaaaaatgc tggaacttta actttatatg ttttattgta agtccatgct atacaagctc
    29761 caacaattat cccaccaaat acacctgcat ttaatgtatc attcaataac tctaaatttg
    29821 acttaaatat catatatgca actacggctg caaccattac acttgtcttg tctttagact
    29881 ttgagtaggc actagctgct cctaataaaa ataagtagtc cacatttgaa aatactatat
    29941 tagatatatt agatatagct ggcacattga gcatatttgg accaaataat aatcctaata
    30001 tacctgctgc tggcattaca gctattggca ccagcatagt ctttcctaaa agtgacagtt
    30061 gttgcatcat tttgtttaag aatctcttca tatccaatcc cctttctatt tcggtttaat
    30121 tttttgtgaa aacgatttat tcacatttta tattttaaat attatatttg tttaaacaaa
    30181 ttgtcaatat tttttattca ttttattttt atcatttagt ttaacatata aatcctttta
    30241 agtgtattgc tatatctagt ctattttttt aattcatttt ttttatttta aaaatatgtt
    30301 taaccatgta tttgaaacaa cccacaaaat aattctattt tgtccttttt atggttgata
    30361 tgattctatt ttcactatac atgtctaaac acatttgata taatttgttc atatcagttc
    30421 aataatatta ctaaagacct ttaaggaggg ataggggtat atggctaaaa ataaattttc
    30481 agagatatat gaagtattga aagaggaaat acttgatggt aaatatacaa gtaatatgat
    30541 gctgcctact gaacttcaac ttattgaaag gttttcatgt agcagaaaca ctgtacgtag
    30601 agcaatttct caattaaaca cagaaggata tgttcaaagt ataaaaggca agggtgttgt
    30661 agtgttagaa aatagttgtt ctaatgattt ttttctaaat atgcacaatt tcaaaggtgt
    30721 agaatcgatt gtagaagata aaaaggttaa tactgctact agtgttctcc acttcagcaa
    30781 aatcttaatt gataataagt tatctaaaaa gacaggtttt aaagttggta gtgaagtata
    30841 ttaccttcac agactaagat atattgataa catacctaaa attctcgaca ttaactattt
    30901 tctatgtagt atagttaaag accttgatgt ttctatagca caaggctcta tttataaata
    30961 tatagaagag tgtctaggta caaaaattgt ttcatcaaga aagatattta aaattgaaaa
    31021 agctacagaa cttgagttaa aaacgctacc tctaaatgat tataattgtg ttggtgttat
    31081 taaaaatagt gtatatactg atgatggaaa gctttttgaa tatactgaat caaagcatac
    31141 acctgaaaca tttgtattca tggatgttac tcaaagatat taaaaaaatt atgtttaatt
    31201 aaacataatt tttttacgtt tagacacttt atcccatact aacctcacta ttctatatac
    31261 aattatatgt ttatacaaat aattatttat tgtatatttt agctttaata ttagtttaac
    31321 gcttgacaat ttgtttaaac atattataat taacttataa attacaggag gtatcaagta
    31381 tgaaaaattg gtggaaaaaa gcaactgtct atcaaatata tccaaaaagt ttcaaagata
    31441 gcaacaatga tggtataggt gatataaatg gtattataga aaaattagat tatctgtatt
    31501 ctttaggtgt tgatttatta tggttgactc ctatgtacgt ttctcctcaa agagataatg
    31561 gatatgatat agaagattat tataatatag acccaaaata tgggacaatg agtgactttg
    31621 aaaaactctt aaaagaagct cataaaagag acataaaaat aatgatggat atggtcctaa
    31681 accatacatc tactgaacat aaatggttta aagaatctaa aaaaagcaaa gacaatccat
    31741 atcgtgatta ttatttctgg aaagatgcta aacctgatgg ttctgttcct aataactgga
    31801 tatccagatt tagtggaact gcttggaaat atgatgaaac cactaaccaa tattatttac
    31861 atttatttga agaaactcaa gctgatttaa attgggaaaa tgaaaaggtt cgtgaagaat
    31921 gttacaaaat actagaattt tgggctgata aaggtattga tggttttcgt ttagatgtag
    31981 tcaacctact ctccaaaaca cctggattac ctgatgaccc tataacagga ccaaaaggag
    32041 atggaagaac tcactacgct gatggtcctc gtatacacga atatttacat aatatgaatc
    32101 aaaaagtttt caaacctaaa aatatagtta ctgtaggtga aatgtcatct actacacctg
    32161 aagagtgtat caactatact agagaaaatc gagaagaact atctatggtt tttagcttcc
    32221 atcatatgaa aacagattat aaaggaggcg caaaatggac taatgaaatg ttctctctgg
    32281 ataaattaaa aaaagcacaa tctgactttc aatataaaat gtatgaagga aaaggatgga
    32341 atgctctatt ttactctaat cacgaccaac ctagagcttt atctagattt ggagatgata
    32401 gattttttcg tcaagaaagt gctaagatgt tagccataac attatttggt ctacagggta
    32461 ctacatacat atatcaaggt gaagaaatag gtatgacaaa tgcctacttt actaaaatag
    32521 atgaatatga tgatgctgaa agtaagaatg catactatca tatgattaaa tctggtattg
    32581 atgaaaagga ggcattgctt atacttcaac aaaaatccag agataatgcc cgtactccta
    32641 tgcaatggga taatactatt tataaggggt tttctaatca taaaccgtgg ttaaaagtta
    32701 ataatgacaa tattagtgta gaaaatgagc ttaatgattc tcgttcagta ttttatactt
    32761 atcaaagatt aatcaaatat cgtaaacaat atgatatttt tacagaaggt acttatagat
    32821 tgttagatga aaatcataaa aatctatttg tttatgaaag agagtataaa aaccaaaatc
    32881 ttcttgttat aagtaatttt actcatgata atgtagtata taaattacct gaaacttatt
    32941 tgaataaatt aaactacaca atacttgaaa ccaattacac tagagatata attgaaaata
    33001 aaatggaaat aaaaccttac gaaagtataa tgatatatta taaataaagt tatttatgta
    33061 aaaatcggta taacaccctg gtgttatacc aattttacta aattcaaagt aatatttata
    33121 gaatatctat agattacaat aattatttga cttctacatc attatatttc tttgaatata
    33181 ctttagtcat atagaaatat ataggaacta caattatcat cccacctaat ccccaaacta
    33241 tcttcatagg ttcatcaata cctgctttta tcagtaataa tatacttata ataactgcaa
    33301 atattggtat aattgctcct ccaggtattc taaaatttac atctttacca gcatacttct
    33361 ttctcaatat tagtacagct aaacatgtag gaatatattg aataaatcta gatacaacac
    33421 ttatttgaga taatgttgtg aatgaacccg accatgctat tagtaaagtt cctatgacag
    33481 atactataat tgctatataa ggagctccat ttttattagt ttttcttata gacttaggca
    33541 ttaatccttg ttcaaccaaa gcagcagcag accttggtgt aaatatggat gatgcaatat
    33601 ttatacctcc aacagatact atagttccta ttgtaattat atacccacct ataggaccta
    33661 acatcttaat ggcagcatct gcaacaggag tagctgaccc tgctaagcca ttacccaaca
    33721 cccctatact tattcccagt ataagcatat atattactga tactacaaac attaccatta
    33781 ccaatgcttt aggtacattt ttcttagggt tctccatatc ttttgctgca acagctaaag
    33841 attcaaatcc tgtaaaagca taaaataaag ttatagatac tgctacaaaa gctgatgacg
    33901 cactttgacc tttttgtatt attacaaatg gggtaaaatt agaacccttt ataaagaata
    33961 atcctacagc tataaataaa actattggta ataattttcc tatagtcatg atattattta
    34021 agtgcttaga ttgtttcatc cccataaaat ttataatccc aagtcctaca actaatatac
    34081 caattattat atttttagta agtggttctg ctgcttgtgg ccatacagat gatagtgtta
    34141 cagcaaagaa atttgccata gtagcccaag caatcatact aactatccac ttcataaatc
    34201 caacttcaaa tccaacaaaa tctccaaatg cctcttttgc ataaagatag ggccctccat
    34261 cagtatcaaa ccaactacct gcttcagcaa aacataaagc aatagaaaca gctaaaagca
    34321 tacaaaaaac taatactaat atacttgctg gacctatatc tgtataagca tcaccaggta
    34381 acccgaatat acctgaccct attattgcat tgattcctaa tagtacaata ctccaaaaac
    34441 ctaacttaat gcctttttcc attgttattc cccccaaagc caacttaata tatttgtatt
    34501 ttttatttag atgcttcttt aactattgcc ataaatattt ttttcatatt atcataatcc
    34561 ctagtcatca tttctggatg ccattgtatc cctattacaa actcacttcc ttccttttct
    34621 atagcttcaa ttaaaccgtc tttagatgta gctgaaacaa tatatcccgg tgctactttg
    34681 ttgacaacta agtgatgaaa actattaact aaaacttctt tttctcctaa tatttcatat
    34741 aattttgtcc cctcctttat ctgtactgta tgtgtaggta cattagatag atgttgctga
    34801 ttgtgtttaa tataagcacc ttcaattaaa gataaatctt gatacaaact acctccttca
    34861 gttacattta taatttgttc tcctctacat atacctaaaa ctggtttttt catatctaaa
    34921 gcatgtttta ataatttcat atcaaaacta tctctctttg gtgatattgc ccccaactta
    34981 ttatgaggtt cttctttcca tatgagtgga tttacatctt gtcccccaga tagtataagc
    35041 gcatctatat tagatacctg ctcctttata atctcatcat catatacaat cggtacaata
    35101 tatggtatag ctttacacat aactactgat tgtatataat cattatttac ataagccctt
    35161 tcatagccag gaaacattcc tccttcatca attagtaaat tacctgatat tcctattatt
    35221 ggatatttct ttttagccaa tttcttcacc ccttaactta tttgtgaaca ataaaaaatg
    35281 ctacttctct taaagagcaa gtagcatacc aagattttat ttatttgaac attataattt
    35341 attttagaaa tacttttttt gaatacgagt aatataaata actttattta cttatatttt
    35401 attttaatcc tgtttagttt gactttttta aattgtttta agtacaacta ataggtaaaa
    35461 taggcattta gattttcctt tttatcaacc tattaaatac tgtttaatat attttttccc
    35521 cttctattta attcacttcc ctttctacca gattcacata ttatcaaatc cagctctttt
    35581 aacatattaa gtattcccct tacctcacct tctgttatat tgtagcctaa ttcatttatt
    35641 gaattaacaa taccatttct acctatattt ttattaagat tattggcatt ttttaaacac
    35701 tccattacta actttacttt ctctatagat gttctatcct ttaatatact tatttcttta
    35761 ctaaaatctt cagttatatt taataagtta tgaggtaaat catttattaa aataacatcc
    35821 tcagaactca taatgttaat atacatagca gtatttttta attctcttat attacctggc
    35881 cattcatatg cattaataat gtcttgaaca tcttgactaa ttgctctctt ccttttcata
    35941 aaatgcttta acataacttc tatatcttct tttcgctctc ttaaaggtgg tatatttatg
    36001 ggcaatacat ttatcctata gtataaatct gctctaaatc tatctccttt tatcatttct
    36061 aataaatttt tatttgtagc cgcaattact ctaacatcta tatctattac attttctcct
    36121 ccaaccctca ttacttgatt ctcttgtaat actctaagta atttgctttg taagtatata
    36181 ggcatgtctc ctatttcatc caaaaatata gttccattgt tagcctgttc aaataatcct
    36241 tttttacctc cttttaaagc tcctgtaaaa gcaccttctt catatccaaa aagttcactt
    36301 tcaagtaaat tttctggcat agctgcacag tttatcgcta taaatggctg actatgtctt
    36361 ggtgatgcat tatgaataga ctgagccatg agttcttttc ccgttccact ttctcccgtt
    36421 ataagaattg tataatcaga ttcagctatt ttcataccta atttcttagt tctttccatc
    36481 ttcgttgaaa ctgtttttat atcatcaaaa gtatatctag caacttgtcc cttttctcta
    36541 agcttctttg ataggttttg ctccaatttt cttatgtaag ttatttcttg aatacaaaaa
    36601 taatatccag taacccttcc aaaactctct atattatact tatttacgtt taagtactta
    36661 ttattaaatt ttataacttc atcatgtgcc ttactcaaac taaaaatctt tttaatattc
    36721 tcattctcta attcatctat atttttccct ataatattac tttgaatatc tagtatatcc
    36781 cctaaactat tattgcaaat aattatgttt cccttatctg ataccattgc catacctaag
    36841 tttgataaat ttattataga atttaattca tctgctttta tagtaagttc tttatattta
    36901 gtatttattc ctgaatataa actaattatt tcatctgtat acttaattag cctttcagta
    36961 atttctttat catccagttt gagttttgct attatttgaa taaacgttga actatcaata
    37021 tacctatggc ctaaatcaat aacttcttct atatattttg gaactaaact tgcctctcct
    37081 ggcgttatag ctatcttaat atttctatat tcttcattgg gattgtatgg tataaaattc
    37141 agatgattta ttcctatatt gtataaatta gatattgttt ccaatacagt atgcttatta
    37201 tcgtttacta ctaatacatc tattccttct ggcaatgaaa ataacttata tatatccttt
    37261 tgctttatgc tcctatttat agttattatc tttgagacat cttctacata tttttttatt
    37321 tttaatactc tatcatctat cattaccaat attatgtcat cttttattaa ttccttctct
    37381 gttattttat ttaaataata attttttatc attactttat ctttaaatat catttctaga
    37441 tttttattta agaaacattc taatttagac aacccatcag ttactactgc tattgttttc
    37501 atttgattac tccttttaaa atatactcta ctcttttata atatacatct gattttcact
    37561 ttaacagcac aaaaaattgt acatttagta tactaacata ttcctttatt agttttatgt
    37621 ccctaaatat catagttgca atatctatca tatttcttaa acttctataa agcaaaaatt
    37681 gtgccaaatt aataattttc ttaattttat tctaaagttc ctcaatagta tttacagact
    37741 tagcacttat agcttataag tctttatatt attaatctat ttttataggt taattgtaca
    37801 aatcatatat cacttttacc cattaaatgc attataaaaa agatggaata acgattcgtc
    37861 actccacctt ttttgtaata aaaaattgta tatataaaca ctatttacta ctcttctaag
    37921 tacaaacatt atctacatac tataaccttt caccattaga ttctattact tgtttatacc
    37981 agtcaaaact ctttttctta atcctagaca agtctccact tccatcatca tacttatcca
    38041 cgtatataaa cccatatctc ttggccatct caccagtaga tgcacttact aaatctatac
    38101 atccccacgg agtatatcca aataaatcaa caccatcttc tacagcttcc ttcatttgct
    38161 ctatatgtgc ttttaaatac tctatacgat agtcatcatt tatcactcca ttagattctt
    38221 ttttatcata agctcctaaa ccattttcaa ctaccatcat aggtattcta tatctatcat
    38281 aaatttcatt tagtgcatat ctaagccctt cagggtctat ttgccatccc cattcactag
    38341 acttaagata tggatttttc actccaccta atatgttgcc acttgtatta tcttctttag
    38401 aatcactact tacacatgta gacatataat aactaaatgt ataaaaatct acacaacctt
    38461 cttttaaaat ttctaaatct cctttttcca tttttatttc aatattattt tctccaaaat
    38521 atctattcat ataagttgga tattcgccac gcacttgtac atctccacag aaataattca
    38581 ttactctact atctctttga gttttaactg catcatctgg attacaagta taaggataca
    38641 ttgttgcaaa tatttgcata catccaactt gataatttga atctatttca tgagctaact
    38701 taacagcctt agcacttgct acaaattgat gatgtaaccc ttgaaaacga agttgaggta
    38761 tatctacttg gtttataaaa tctgtagtcc cttcatttaa tattccttgt gataagtagc
    38821 ctcccatagg cattgtagct gaatttattt cattaaatgt cagccaatat tttacttttc
    38881 ctttatatct tttaaatatt gtttcacagt aattcatata caagtctatt acatctcttg
    38941 aaacccatcc attgtatttc tctgttattg caaatggtat ttcatagtga gatattgtta
    39001 ttaatggttc tatattatat tttaaacatt catctatcac attttcataa aatttaagtc
    39061 ctgcttcatt tggttctttc tccattccag ttggaaatat tctagtccat gctattgaaa
    39121 atctaaatac cttaaatccc atttctgcaa ataaagctat atcttctttg tatctgtgat
    39181 aaaaatctat tgcttcatga cttggataaa atgttccttt ttctataact ctcgttattc
    39241 ttttacttac tttttgagaa cctccagtac acatatctga acaagaagga cctttcccat
    39301 ctaaattcca tgctccttca cattgatttg ctgctgttgc tccaccccat aaaaaatctt
    39361 ttctaagtgc cataattcta cctccaataa tttatttata gtaactttat tttaaaataa
    39421 aaatacatat atataattct taattaatag aactctcttt tacgctaaaa cagttatcaa
    39481 ttcctgacta ttatttacat gcttttgtgt attttctaaa atatccacat aatcagaata
    39541 attagtaaca actactggtg tttctaagca atatccttct tttgaaatac tttctatgtc
    39601 aaaagttatt aatatctgac cttttttaat cttatcccct tgattaacaa aagatttgaa
    39661 atatttacct tccaactgaa tagtatttaa ccctatatgt atcaatagtt cacaaccatt
    39721 atcagatata attcctattg catgttttgt tggaaataaa gtcattacag tcccatcaaa
    39781 tggtgccact acttttcctt ctttaggtgt tattgctaca ccttttccca atatacctgt
    39841 tgaaaacgct gcatcttgta tctgctccaa agcctttact tcacctttta ttgggctata
    39901 tactacttct cttttgtttt tttctataac gccttgccca ttatcatctt tagaaatttc
    39961 tactatagca tcatctttcc aaaaaaataa tgttaaaaca aatcctacta caatagctac
    40021 tattattgct attatagttg cataaacacc tttcatatct ccagttgttg gatttatata
    40081 attaggtata ccaaatattc caagtccacc cattacataa ctttttacat tcattatccc
    40141 cattacagct cctgccacac caccacctac acagctatat atgaatggtg tttttcttgg
    40201 taaagttatt ccatatattg caggctctgt tacaccacat atccctgaaa taactgcagg
    40261 tatacacagc tcttttatct tcttatcttt taatttaaag tacattgctg ctactactgc
    40321 tgtttgtgca aaagatggta caaatgctcc cactaatatt gtgtcaaacc ccatagtcat
    40381 aaggtttgac atagctattg gtactaatcc ccaatgtaga ccaaatatta caagcacttg
    40441 ccacaataaa ccagttaatg caccacataa aattggtgaa aagttaaata aagctaaaaa
    40501 tccatttcca actatattag aagcataggt agctataggc cctataacca taaatcccaa
    40561 tggtaaagaa actaataaaa ctgccattgg tacaaagaaa ttctttacta catctggtat
    40621 tattttagca aacactttct ccaacttagc tgctaataat actattaaaa ttactggtac
    40681 tactgttgaa gtgtagttca ttgctattac tggtaatcct aagaaagtta tatatactgg
    40741 tgattcaaat attgttccag taaatagatt gtataaaggt tgacctgtag atgctagagt
    40801 atctaattga atacctgaat agcataaaac tgcacctata agcattccaa taaatggttt
    40861 taatttgaat ttcttagcag ctgtatatcc aagaattatt ggcatatact ggaacattgc
    40921 atctccaatt gcatttaaca taatatatgc accatcagtt tctccataca aacccaatgc
    40981 tgtaaatact gcattaaatc cttttatcat accagcagca cacagaactc ctaatattgg
    41041 ttgaaaaact cctgatatta tgtctataaa cttattaagt aatccttttg gcgcatctga
    41101 gtctgaatta tctattgtat ctcctcctaa tccagccaaa tcgcacacaa cttcataaac
    41161 atctggaaca tgattaccaa taactacttg gtattgacct gcacttttca ttacagtaac
    41221 tataccatcc atttttttta gcacatcatc atttgctatt gactcatctt ttaatttaaa
    41281 acgtagcctc gttatacagt gagataaact gctaatatta tcctttcctc ctacattcgc
    41341 aatgatttct tttgatagtt gttcatactt tctcattttt tctcctccat tttctttttg
    41401 aaatatgaag gtttggccaa cttaatgtca ccacccaata tatataaacc taattattag
    41461 gatttatatc tgacttaata cttttactta ccactctttc aacgtgaata gttaagtata
    41521 atttttcctc atctgttaaa tcgtagttgt attttttatg tataaattca tccaactttt
    41581 ctatgaattt ataagcattt ttatatttaa cttttattac atttaataaa tcatcttcat
    41641 tattttcatg taaattatta ctaacaattc gttctgcaaa atactttaag tgagtaataa
    41701 atctatgata ataaactgaa tcttcatcaa atactattct gtaatggtat ttaaccatat
    41761 ttgaaatttc ttgtattatt tgtgtaatct catatatagt ttttatactt tgatccattt
    41821 gtgcattaac tatatgcagt gctataaatc cagcttcatc ttctgatagt ctaatttcaa
    41881 attttttttc tatataatcc aatgcctcta accctatttt aaattcactc ttataaaatc
    41941 tttttatatc ccaaagtaat acatttttta cacttactcc ttcacgagct ctttctattg
    42001 cagtatacat atggtctgtt aaagaaatat aaatactatc atttaatctt ttccctagtt
    42061 tttctttagc atataaaatt atttcatctg ataattccat atatcttata ggaatatctg
    42121 ctattagttc tttaaattta tcagatatat ttgggtctga aagtttgaat atcttatcaa
    42181 ttttatcctt tgatatatgc tctcctgtac gttttttata agctaaacct ctccccataa
    42241 ctactatttc cttatgattt tcatcagttg taataattac attattattg agtatttttt
    42301 caataatcat tgtgatgccc cctttattag cattaagtta aaataaatct tttttaatta
    42361 tttagaatat ttaaaatgta atttattaca taaaaaaaaa cctatttatt acacattaaa
    42421 tagactccca tgagtcctga gtaataaata ggtttagctt gtatttaaac aatcactatc
    42481 cgtttgtatt ttattttaat tattatttat tatatgttag caaatttatt ttttagtgtc
    42541 aactttttct caatatttta attttaaata aattatataa attgaatctt caaagtacta
    42601 tttttatcaa taaacaaaat aaaaaaggta ttactttctt taagtaatac ccatttattt
    42661 ttaatttaag tggtttttct taatctcatc aagtagtcca tcttctaaat aaatttctaa
    42721 tgcacatcct accattgcct ttactgcttt gtgaagttta tcataagctt cctcagaatt
    42781 tgcataattt agaaattctt tttcatgaac atataccatt ggacttatat ctaaggctat
    42841 ttctgtatac atagtagggc aaacttgact tacattacct atatctgtag aacctactgg
    42901 accatcttta ccttctaata tatctgttat acctgcctca atcaaattat tctttgttat
    42961 cttttgaagt actttgttat ttattatgtt gtcaaaagaa ttttcaaagt atctataaga
    43021 aagcttagct cctgtcatta actctgcacc ttttgcacag tttataactt ttttagttac
    43081 ttcatctaaa taacttcttt cagcagctct tacgaaaaac ttacaagctg ctttttctgg
    43141 aactatattt ggagcctctc ctccatttga aactatacca tgtattctaa catctgattt
    43201 tacatgttgt cttagtgcat ttatcccagc aaaagtaagt tgtactgcat ctagtgcatt
    43261 gactcccata tgagggtgac tagctgcatg tgctgatacc ccttcaaaac taaactctaa
    43321 tgaatctatc gccaaagcct tacagtttat attattaaaa gcttctatat gcatttgata
    43381 acttacatca acatcatcaa aagctcctgc cttaactaaa tcacattttc ctcctgttgt
    43441 ttcttcagct ggtgttccta ttagtacaat ttttccttta aaattttctt ttaacttact
    43501 taatactata caagctccta gtgtagatgc tgcaatccag ttatgtccac aagcatgagc
    43561 tggttcttta ttttctccat atccaggaag tgcatcatac tcagctaaaa atgctatggt
    43621 aggtccatca ttatctccta attcagctct aaaagcagtc tccatattac aatatggata
    43681 aactgtatca aatccatatt ccttcatttt ttctactaag tattttgatg atatgtattc
    43741 ttcttcccct aattcaggat tgttaaatat aaaattacaa acttcttcaa tttcttttcc
    43801 aagttcattc tctatctcat aagctctctt ctttatactc tctatcataa tttacctcct
    43861 actagaatcc tagatataat cctattgtca aaaatgccat tacaagaatt gttaatacta
    43921 ccattaaagg aattacgaat ctaacccact ttgaccaagg aatattggct actgctagtg
    43981 ctcccattaa aacacctgat gtaggtgtta tcaagttagt tacacctata ccaaattgat
    44041 aagctgtaac tatcatttgt cttgatacat ctactaagtc gccaagtggt gcaagtacag
    44101 gtattgtaag tgatgcaagc cctgatgctg atggtattaa aaatgctatt aatccttcta
    44161 ttaagaaagt taaatttata aatatagttt ttggtaatcc atttaataaa tttgctgcag
    44221 aatttaaaat tgtatctatt ataaatccat tttctgctat tattacgatt ccacgtgcaa
    44281 atgctataca caatcctgct gtaattaaat ctcctgctcc acttataaat gaattaacta
    44341 tctcatcttg tttaagccct gatgctattc ctgatattat acctatagct gtaaatatca
    44401 ttgcaatttc agttatgtac cattctagtc ttaaaactcc atatatcata atcgccatac
    44461 ctattgtgaa aatggctatt accatggctt ctctttttgt aaattccttt atatttgtat
    44521 cactattaac cattaattct tgatttagtg atatatcatg tactaaagat tttttagggt
    44581 cttttttaac tttattggca tacatcataa caaatcctat agatatagcc atatatatta
    44641 taaacataac aactctaaac tctattccag aacctggtac tatttgtgat aatgcttgtg
    44701 ctattcctac agagaaagga ttcgtagtag ctgctccaaa tccagctgtt gctcctataa
    44761 atactgtggc cactgctgtc aatgaatcat atcccatact ggtcataagt ggaataaata
    44821 tcatataaaa aggtagtgtc tcttcactca ttccaaacgt cgtcccacct agtccaaata
    44881 gaatcattga aatcggtatt attaaaaatt gaaacttctt cattttattt acagtatgag
    44941 ctataacagc ttctatagct cctgttttat taactatccc aaaagctcct ccaactatta
    45001 aaacaaatcc aactacttca gctgcatcta taaacccgtt tataggtgct gtaaatacat
    45061 cagtaattcc ttgtggattt gatggtgctt tttcataagt tccagctaca acaactgacc
    45121 ttccgtctat atctactctt tcaaaatttc cacttggcac tatccaagta agaattgcca
    45181 ttaacactat tagcaaaaat attattgtaa gtgtctgtgg catagtaaac tttttctttt
    45241 ttaaactgct catttttaat ctccccactt tttaaattaa atattttatt attgcatccc
    45301 tttatcctat tttgtataga tgtaaaaaag ctcaacatgg atacatgttg agctaaatgt
    45361 gctaaataca acatacctgc atcttccctt tatcttgttt aagatagctc tccatcaaaa
    45421 ttcgggataa ttttgatgac agtactgtac ctattaaata cagtcccagc actagcttcg
    45481 gacactagca cttcggcact tactcctttt atattgcctc attggtttcc aacctcttca
    45541 atattaatct tagtaaatgc aacctctacc tcaatctgtt tgttagatga ggtaattctt
    45601 attaattttt attaattcaa agtaattgta gcataatatg tcgagattgt aaatattttc
    45661 agtataataa ttcttacttt atgatttcat tccatttttc acaaataatt ttttctccat
    45721 ataatttaaa tactgtagta tattttaaat acacacaaac tttccacaaa ttttaaatta
    45781 ttataatacc tttataataa aaaataaaag ctatgtaaac ttatcaattt acacagcttt
    45841 atatacatta atctactcca agtacagctt taagctttga tgctattgta ataactcgtg
    45901 gaccaaatat aacttgaact gagtttttct ttttaacaac tccacttgca cccaattcat
    45961 ttacccagaa gccatcttca tagaatccat caattgaatt tagctctacc ctaagtctag
    46021 ttgcacagtt tgtaacattt tttatattat tacttcctcc taaaccttct attatagaat
    46081 gtacaagctt tgtatcttta tcatctactt cctcttctac tgcattgttg tatgctgttt
    46141 taccagcatc tacagattgt ctttctctat attctttctt actataaagc ttaacttctt
    46201 ctgaacttct tcctggtgtt tttatatcaa acttcttgat tgcccatgta aatattatat
    46261 aagtaactat aaaaaatatt ggtatcaaaa ataataatgg taacgcatcc actttttgtg
    46321 gttgtaataa attaggaaac atatttctaa atgattctcc cattatagaa acattaaaca
    46381 tttctgtaaa tacatacgtc aatcctgcca gtggtacata tgctacaaaa tacaataatg
    46441 gtgcaacaaa taggaatgta tactctattg gttccgttac ccctaccaac atggctgtta
    46501 ctattgctgg tatcaatata cttattgctt ttttcttatt agcattatca gccaacttat
    46561 acatagctaa agctgctgct ggtaatattg caaaatgtat taataaacga cctgttgtga
    46621 agttacgagt tatataagat agtgtatctg ggtctcccat ttgagctaat cttatattat
    46681 taacaccctc gtaaattgct ccacctactt ccattgttcc acctactctt gtatattcta
    46741 tagggaatgc aattaagtga tgtataccaa atggtaataa tgctttatcc attgctccaa
    46801 aagtaaatgt tcctaataat ccactagaac ctatccaatt accaagttta gataatgcca
    46861 accctactgg tggccatata aagtacattg ctgtacccaa tactattgat gctataaaca
    46921 tcataatcat tgaaaatctt ggacctgaga aaaaagataa tgcactagga agttctatgt
    46981 caacaaattt gttatgtact gcactagcta caaatccagc tattatagct ccaaatatac
    47041 tacaatcata tgaaaagatt cctaagaaag aaccatataa agaacttcga ctatatgctt
    47101 ctgcctctcc taatccagat gccataaacg cttctgctgt aactgtatct gaatttatac
    47161 catttgctgc taacaatgta cttataactg tgtgcattcc tataaataaa acaattcctc
    47221 ctaatgcacc ccatcctttt tcctttttag ataatccaaa ggctatacct gtaacaaaga
    47281 atattggtaa gtatctcatt accataaatc ctaaatcgtt tattacttta aagaatacat
    47341 ttataaatga cccttctttt attagtccgc taagccctaa tgcagaaacg ttttccatat
    47401 ttgcaaaagc tgaccctaat cctataaata ctcctgctac aacaagtaat ataacaggta
    47461 tcatcattgc tccagaaagt acttgtaatt tatccttcat agtattattt cctccatttt
    47521 tattgaaatg ccaggctata taatgcatat atatatactt ctattgcttt gtataatgta
    47581 tctatactta tattttcatt tgcctgatgt gaatttggat tatccccctc tattgtgggt
    47641 ccaaatgcaa ctccattttt taacactctt gcataagatg cagctccttt tgtaaaaagt
    47701 tcggcttctt ttccaaaacc ttgtttatat gcatttgata atttatttat taattcacta
    47761 tctggtttta catacagtaa ttttaaatcc ttatatatat caactattaa attttctttt
    47821 tttccaattt cattaattct attagttata aactcccaac ttataccctt aggatatcta
    47881 aagtctattt tcatattcaa ttcatttttc tctaagaaga tactcattat acatattgta
    47941 gaaactccca tatctacatc ttctttatac atccctaatt tttttccatg attatcatct
    48001 gtaaagtatg aatttaaaat atcccttatt ataacgcctt tatcatttag ttttttaatt
    48061 ttctctaaat cttttgctaa actaaatgca cagttatatg acctgtgtgg atttctcgat
    48121 aaagcccttt ctcctgtata tcttactaat actttacctt catctaattc ttctatctct
    48181 gtataataag ctaatgattc tactaaatct ttcttatcat tagttttaaa aacagcctca
    48241 attttgtcac atacaaatcc atcttctttg tttgacttta tatcaactaa attgtgattt
    48301 ctaaatttat ctttatctat tttctttctt agattgaagt ataaaatacc tttttctcca
    48361 tttactattg ggaagtttcc atctggagaa aatgcaaatt ttggctgttc attgacacta
    48421 aagtaatgct ccatacattc ccaagtcgtc tcttcagctc cacccactat caatctaatc
    48481 tttctcttcg gcttttcatt aagttctctt aagaaaagta atgcatataa aatccctatc
    48541 aaaggtcctt tgttgtcatt aactcctcta ccataaagat agttatcttt ttgacatact
    48601 ttaaaaggtt tactcttcca aagttcttta ttatatattg gcaccacatc tatatgatta
    48661 agcaccccta ctacttcttc tccttctcca tattctatat gaattgcata tccatcaaag
    48721 tcttttacaa caaaatcttt gtcttttgct atttgaataa gcttatcgaa ggcatttctt
    48781 atttctatcc caaatggttg atttatatct gttgtacttt catctctaat tgaaggtatt
    48841 tcaactaaac ttctaatatc atttatgata ttttcttgat tggattttaa ataatcaata
    48901 attagttctt tgtttactat catcttaatt atttatcacc cctttatatt aattttacaa
    48961 agcatttcag ttcatatcta cattataaat tttgaatatt attttttcaa tatttcaaat
    49021 acttctctaa gttaccataa tgatacggta aacaatcaac agattatgac aaaatcatac
    49081 tatacctatt actaatattt taacttatct attacgtctt ttttaatgta agtttattcc
    49141 aaaaatacat aaaaaaagat gtagccttag ctacatcttc ttatatctat tcaacttcca
    49201 ttattatgct attaatatct acatttccag gttctatcct atcttttact gttctcttaa
    49261 cagaatcagc atcatccatt ataactacag gagttattat atttaaatca tttgacgtta
    49321 taaaatctaa atctacttcc attattaaat ctttagcttt taccttatct cctgccttta
    49381 ccttaacatc aaacggcttt ccttctagtg acattgtctc taaacctaca tgtattaata
    49441 tatttgtatt atcattactc ttaatcatta ttgcatgctt tgtatcaaat acattgacta
    49501 tttcaccatc aacaggagaa tataacttac cttcacttgg tattattgct actccatctc
    49561 ccatcatctt ttgggcaaaa actgcatctg gaacatcttc taagttcact aaatttccct
    49621 taaaagggct atatattttc tcaatcttat tttctttttt cttaaatata ttaaacataa
    49681 cacactctcc acattataaa attttatgat aatatttttc ttaaagcttt tgctacacca
    49741 tcatttacat tagtatcagt tacaaaatct gccatagatt ttacttcatc tgtagcattt
    49801 cccatagcaa taccaagacc tgcaaattta atcatggata tatcattttc attatcacct
    49861 atacaaataa cttctttgct atctatacct aatatatcac aaaatttttt aactgcaata
    49921 ccttttgaag tacctttact cataacttca aagtttatct ttccagaact tactacttct
    49981 aaatcttctt gttttttaaa ttcttccttt gcttctctca aagaatctac gttttctgag
    50041 aaaagtatac attttaaaat ttcattatca gctttttcta ataaagtttc acaacttgca
    50101 ttttctatta tttcaattct gtcttcttct ttatttttac tatttattaa tctatatgga
    50161 tgttcttcac ctatttcatt gttggatata actattttac agcctttaaa atgtttgttt
    50221 aaattatact tttcacctat tgtatagatt ttctttagag cttctttgct caaaaccttc
    50281 ttatactcat atcccagtaa tttaaaatat gtcccattag ttgatataat atatatatca
    50341 tcccctaaaa cacttgtaaa atatttcata gcattataag gtcttcccgt aactaaggct
    50401 accttgatac ccttttccat ggcttctttt agtgctttaa tattttcttc tgatacttca
    50461 aatcctttac ccatcaaagt accatccata tcagtacaaa ttaatttata tcgcatttgt
    50521 atcacatcct agtaagattt ttctaatatt tcaacaaggt cttctttctt tccaaaaggt
    50581 aaaaatccta aaactggaca gcttgttatt tgttctaatt gttcctttgt tacattaaac
    50641 tcagacattt ttgtcttaag ccccactctc tttaaaaaac tttctatttc atttcctaaa
    50701 gttttagctg cttcatcatc ttccactttt tctaattctt ggtttagtat tctcgctact
    50761 ttggcaaatt tctctatatt atccttatag aacttttctg caaattttgg gaacactata
    50821 gccaaagcct caccatgagg aatatgtgta actcctccta ttatttcact tagtggatgt
    50881 ggtgctgctg ctccagcatt tgccagtgaa cttcctccta atgtatctgc tactgctaac
    50941 tttgttctgt attctatatt atttttatct tccattactt taggtagata gttaatgact
    51001 aattccatag atttttcaga cattatctct gtaaaatcat tagcatttgg atttatataa
    51061 ctttcaaatg catgagtaaa agcatcaaat gctgttgatg cagtaattct ctctggcaga
    51121 gttaacataa gctctgggtc aactatacat tcttttgaga aacaattttg atgaaatatt
    51181 gtatttttct cgcttcctct cgatattact gctgcttgtg taacttggct tcctgttcca
    51241 gaagtagttg atacagctat taatggtaat tctttttcac ttaccttatc gtatatttta
    51301 aatggatttg tatatgtact aaacatataa tcccaatcta ttttttctaa accaaaagtc
    51361 aatgctaata cttttgcagt atctatactt gaacctcctc caacagctaa aacaacgtct
    51421 acagattctt ttttagctaa ttcaaaccct tcgtttatta attctacagt tgggtttggc
    51481 tctactctct gaacctccca tgactaaagt cacagggttc ctgcttcaca gaattttgca
    51541 tactaaaata tgtcttacta attctccaca ggccatcccc gtagttccta cggttcttac
    51601 ataagttatt tattttgaat tgttattagt cttaatcctt cttttaatat atttatactt
    51661 gcatttatat ctctatcgtg agtttcatta caactaggac aaatccattc tcttatattt
    51721 aagtccttca cttcctcgtt tttataccca catttattgc atatttgtga acttgcaaag
    51781 aatttaccta cctttacaat ttgtctacca tgccaattag ccttgtattc taattgacga
    51841 ataaattctg accatgatac atctgaaatt gagcgagata gtttacgatt tttaatcata
    51901 ttctttactt gtaaatcttc tatgcaaata atatcattct cttttattaa tttagtagat
    51961 aatttttg
//
LOCUS       DAYASV010000013        51417 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYASV010000013 DAYASV010000000
VERSION     DAYASV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.013"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..565
                     /locus_tag="ACGI68_000796"
     CDS             <1..565
                     /locus_tag="ACGI68_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HFH0137244.1"
                     /translation="TMLILISHINILIIADIKNKDICLKLNIKYMFNLININRQLYPA
                     ENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFGTGNIGLTSSV
                     YVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKALFNIIIMINKI
                     MNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            549..947
                     /locus_tag="ACGI68_000797"
     CDS             549..947
                     /locus_tag="ACGI68_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HFH0137245.1"
                     /translation="METTLETIKGSIDANTIIGDPIKTDTTVVVPISKVTIGFGIGGG
                     EYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETRIMSLDSNINL
                     VDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1195..1635
                     /locus_tag="ACGI68_000798"
     CDS             1195..1635
                     /locus_tag="ACGI68_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0137246.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDINNKDKLFTSLEESKNIIEQLF"
     gene            1651..3102
                     /locus_tag="ACGI68_000799"
     CDS             1651..3102
                     /locus_tag="ACGI68_000799"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFH0137247.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGCFVEGILLGNYKFDKYKTKSKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALILLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3193..3861)
                     /locus_tag="ACGI68_000800"
     CDS             complement(3193..3861)
                     /locus_tag="ACGI68_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HFH0137248.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            4130..4270
                     /locus_tag="ACGI68_000801"
     CDS             4130..4270
                     /locus_tag="ACGI68_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137249.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4445..4765)
                     /locus_tag="ACGI68_000802"
     CDS             complement(4445..4765)
                     /locus_tag="ACGI68_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFH0137250.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(4885..5622)
                     /locus_tag="ACGI68_000803"
     CDS             complement(4885..5622)
                     /locus_tag="ACGI68_000803"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFH0137251.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5837..7660
                     /gene="acd"
                     /locus_tag="ACGI68_000804"
     CDS             5837..7660
                     /gene="acd"
                     /locus_tag="ACGI68_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HFH0137252.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISEGNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7875..12173
                     /locus_tag="ACGI68_000805"
     CDS             7875..12173
                     /locus_tag="ACGI68_000805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFH0137253.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12377..12838
                     /gene="rimP"
                     /locus_tag="ACGI68_000806"
     CDS             12377..12838
                     /gene="rimP"
                     /locus_tag="ACGI68_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFH0137254.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            12868..14022
                     /gene="nusA"
                     /locus_tag="ACGI68_000807"
     CDS             12868..14022
                     /gene="nusA"
                     /locus_tag="ACGI68_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFH0137255.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            14029..14310
                     /locus_tag="ACGI68_000808"
     CDS             14029..14310
                     /locus_tag="ACGI68_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFH0137256.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            14300..14611
                     /locus_tag="ACGI68_000809"
     CDS             14300..14611
                     /locus_tag="ACGI68_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HFH0137257.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14633..16573
                     /gene="infB"
                     /locus_tag="ACGI68_000810"
     CDS             14633..16573
                     /gene="infB"
                     /locus_tag="ACGI68_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFH0137258.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16616..16996
                     /gene="rbfA"
                     /locus_tag="ACGI68_000811"
     CDS             16616..16996
                     /gene="rbfA"
                     /locus_tag="ACGI68_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFH0137259.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            16977..17960
                     /locus_tag="ACGI68_000812"
     CDS             16977..17960
                     /locus_tag="ACGI68_000812"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFH0137260.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLRFLVEDVKIYKSNEFNMKNYSLIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNVHASSFEMAKNLLVLGAQKNTIIQNIYQSNSSGYYKLLGEA
                     LKGLEIFDSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYVDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            17973..18878
                     /gene="truB"
                     /locus_tag="ACGI68_000813"
     CDS             17973..18878
                     /gene="truB"
                     /locus_tag="ACGI68_000813"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFH0137261.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            19009..19938
                     /locus_tag="ACGI68_000814"
     CDS             19009..19938
                     /locus_tag="ACGI68_000814"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFH0137262.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            20042..20299
                     /gene="rpsO"
                     /locus_tag="ACGI68_000815"
     CDS             20042..20299
                     /gene="rpsO"
                     /locus_tag="ACGI68_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFH0137263.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            20539..21384
                     /locus_tag="ACGI68_000816"
     CDS             20539..21384
                     /locus_tag="ACGI68_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0137264.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            21509..23620
                     /gene="pnp"
                     /locus_tag="ACGI68_000817"
     CDS             21509..23620
                     /gene="pnp"
                     /locus_tag="ACGI68_000817"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFH0137265.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            23886..24629
                     /locus_tag="ACGI68_000818"
     CDS             23886..24629
                     /locus_tag="ACGI68_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0137266.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKAGKLSDVFK"
     gene            24787..26034
                     /locus_tag="ACGI68_000819"
     CDS             24787..26034
                     /locus_tag="ACGI68_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0137267.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINSVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            26069..26314
                     /locus_tag="ACGI68_000820"
     CDS             26069..26314
                     /locus_tag="ACGI68_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFH0137268.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            26324..27547
                     /gene="dapG"
                     /locus_tag="ACGI68_000821"
     CDS             26324..27547
                     /gene="dapG"
                     /locus_tag="ACGI68_000821"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFH0137269.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            27660..28394
                     /locus_tag="ACGI68_000822"
     CDS             27660..28394
                     /locus_tag="ACGI68_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFH0137270.1"
                     /translation="MKDYREDNERFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            28517..30928
                     /locus_tag="ACGI68_000823"
     CDS             28517..30928
                     /locus_tag="ACGI68_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFH0137271.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            30931..31989
                     /gene="mnmH"
                     /locus_tag="ACGI68_000824"
     CDS             30931..31989
                     /gene="mnmH"
                     /locus_tag="ACGI68_000824"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFH0137272.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNDVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            31983..33317
                     /gene="rimO"
                     /locus_tag="ACGI68_000825"
     CDS             31983..33317
                     /gene="rimO"
                     /locus_tag="ACGI68_000825"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFH0137273.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHKIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            33304..33846
                     /gene="pgsA"
                     /locus_tag="ACGI68_000826"
     CDS             33304..33846
                     /gene="pgsA"
                     /locus_tag="ACGI68_000826"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFH0137274.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            34134..35180
                     /gene="recA"
                     /locus_tag="ACGI68_000827"
     CDS             34134..35180
                     /gene="recA"
                     /locus_tag="ACGI68_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFH0137275.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGTSVSKEIVEE"
     gene            35356..36897
                     /gene="rny"
                     /locus_tag="ACGI68_000828"
     CDS             35356..36897
                     /gene="rny"
                     /locus_tag="ACGI68_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFH0137276.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            37211..39547
                     /locus_tag="ACGI68_000829"
     CDS             37211..39547
                     /locus_tag="ACGI68_000829"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0137277.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYSYDSVFLVSTKTNNYYHFNGLDRNLSANNSENQWYYTFLKNDDEYSLNVDNDEA
                     SNNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYNDKFDVVARLIDDKGFVQ
                     LAVDKTGHENINFFENSSSDLSNSKQLILNNKKEQKSFWYSSEKSKSYIVCQYIPSLK
                     WHLVLENDFTLMIKQLHLQFFKGIAVIVLIIIFVLFTITHVLKKYKNQIIKLSVSQKL
                     EYQKLLSKSTEGLYENIFEIDITNNKAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKRYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDNFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     NNNYVLIDKLERFCSKINQKKFVEQAEFSIACSIGVAMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHIFSEDSIDSISFHIDSRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSIGFDIFSPVLQDDLQMVLDTLREAISKRETFEIVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISSINTLLENK"
     gene            39929..41710
                     /locus_tag="ACGI68_000830"
     CDS             39929..41710
                     /locus_tag="ACGI68_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFH0137278.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
     gene            complement(41792..42322)
                     /gene="lepB"
                     /locus_tag="ACGI68_000831"
     CDS             complement(41792..42322)
                     /gene="lepB"
                     /locus_tag="ACGI68_000831"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0137279.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(42362..43186)
                     /locus_tag="ACGI68_000832"
     CDS             complement(42362..43186)
                     /locus_tag="ACGI68_000832"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0137280.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            43443..44351
                     /locus_tag="ACGI68_000833"
     CDS             43443..44351
                     /locus_tag="ACGI68_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0137281.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            44495..45940
                     /gene="purB"
                     /locus_tag="ACGI68_000834"
     CDS             44495..45940
                     /gene="purB"
                     /locus_tag="ACGI68_000834"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFH0137282.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            46251..46703
                     /locus_tag="ACGI68_000835"
     CDS             46251..46703
                     /locus_tag="ACGI68_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0137283.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(47038..48234)
                     /locus_tag="ACGI68_000836"
     CDS             complement(47038..48234)
                     /locus_tag="ACGI68_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0137284.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            48619..49569
                     /gene="galT"
                     /locus_tag="ACGI68_000837"
     CDS             48619..49569
                     /gene="galT"
                     /locus_tag="ACGI68_000837"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFH0137285.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIDSEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            49730..50482
                     /locus_tag="ACGI68_000838"
     CDS             49730..50482
                     /locus_tag="ACGI68_000838"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFH0137286.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(50574..51350)
                     /locus_tag="ACGI68_000839"
     CDS             complement(50574..51350)
                     /locus_tag="ACGI68_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0137287.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKSSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMWKTNQFTLENVKQRKNK"
BASE COUNT        20651 a         5581 c         9170 g        16015 t
ORIGIN      
        1 aactatgttg attttaatat ctcatataaa catcttaata atagctgata taaaaaacaa
       61 agatatttgt ttaaagctta atataaaata catgtttaat ttaataaata taaatagaca
      121 actttatcca gctgaaaata gtaaaaataa tgataaaaaa gaggggatga agaataatat
      181 agattcaagt atattattag ctgatgacct tttgagcatt tatagattat taaaaaagat
      241 aaaaatacat gaactttatt caaatattaa ttttggaact ggaaatattg gcttaacatc
      301 ttctgtatat gtattgataa atactttgta tggaaattta tttaatatga ttgatgcaga
      361 aaaaatgtat ttaaatgtaa atccagattt cacaaaagat tatgttttag gaaatataag
      421 gatacatata aggccaagaa taaaagcttt atttaatatt ataataatga ttaataaaat
      481 tatgaataaa aataaaggaa ataaggaagg tgatagtaat gagagcaaca ggttcgatac
      541 agagtcttat ggaaacaact cttgaaacaa ttaaaggttc tatagatgca aatactatta
      601 taggagaccc tataaaaaca gatactacag tggttgtacc tatatcaaag gttacaattg
      661 gctttggtat aggaggagga gaatactcaa aaggatatga tgataaagat agggaaattg
      721 aattaaaaaa tgaaaaatca gatacgaatt ttgctggagg tagcgcaggg gctatatctg
      781 tacaaccagt agcatttgta gtagttgaaa gtggagagac tagaataatg agtttagata
      841 gtaatatcaa tctagtggat aatatattat ctataactcc tagagtattg gagaaaattc
      901 aaaatatatc acaaagtaat aagaatcaag ataaaaacaa tatgtaagaa taaaatattt
      961 ttaaatatgt atatagtttt aagtaaagta gtggggttaa tataaatccc actattttgt
     1021 tgtgtacttt aaacatttat atctaataag attttagttt aaaacaattt aaatagataa
     1081 taaagttgat atatagtata attataaaaa gaaaagaagt attatattat agatatataa
     1141 acgcatttga gatataagag ggagttataa atcattagtt ttgaggtgaa tgttatgagc
     1201 gataaagaat tatctatttt gttgttagaa agtatgaata attttcataa tttaataaag
     1261 attattaata acgaaagata taaaaaagat aaggttctta cagaaagaca gttctttgcc
     1321 ttagtaaaaa taagaaaaca tgataaaata gagcttaaaa atctaagtag agatttgcat
     1381 gtatctactt ctagtctgtg tatactactt aataaattgg ttgagcaaga gtatgtatat
     1441 agggaagaag atagtagaga tagacgaaat actttttatg gaataactaa aaatggagaa
     1501 aagatattgg ataatgaaat actaaagttt gtatctataa taagtgataa aatggattgc
     1561 ctagatataa ataataaaga taaattattt acttctttgg aagagtctaa aaatataata
     1621 gaacaattat tttgaaaagg agaatttatt atgaatataa agtattctaa agaaatatta
     1681 aaaagtttag aaaatgctat aacagtagta gctctatttg agggagaaga tgtttctacg
     1741 acatttgata aaataaatat gataagcaga aattttaatt ctaaagaaaa tagtatagat
     1801 actgtaacat tggttgaaaa taacaatttg gtaaatgttt gtcttgttgg ttttggtaag
     1861 agagagttat tgaatagaga aaaaattaga atcataggtg ggaatttgtg taaaaaatta
     1921 aaaaatatat tgaaaaatga agcatataag agtagtgatg ttaatgtact gaatcttaat
     1981 ttaaatagtg atgaaatagg ttgttttgta gaagggatat tacttggaaa ttataagttt
     2041 gataaatata agacaaaaag taaggatttt gaaagaaaag aagttgatac tataactata
     2101 tttactgaaa aagaagttga gtgtcaaata gaaaaggcta aaatattagc taattcaact
     2161 ataatagcta gaaatctagt taatgaacca tcaaacgtaa tatatccaaa aacattagca
     2221 gtagaaacag ttaaacttgg tgcagagttt gggtttaatg ttgatgttta tgaagaagaa
     2281 aaaataaagg cattgggaat ggatgctttt ttcgctgttt caagaggttc aattaataaa
     2341 ccaagattca tagtaatgag atactttgga gataaagaaa gtacagatat attaggtcta
     2401 gtaggtaaag gattgactta tgatactggt ggatattctt taaaatcaaa tgcttctatg
     2461 ctagatatga agacagatat ggctggtgct gcgagtgtta taggtgctat gtgtgctata
     2521 tctcaaagta aattaaagaa aaatgttata gcagtagtgg cagcatgtga gaatgcttta
     2581 tcaggaggtt catacaaacc tggagatata attagttcta tggcaaagaa aactattgaa
     2641 gttttaaata cggatgcaga aggtagactt acattagctg atgcaatata ctacattata
     2701 aacaatgaaa aagtaacaaa ggtggttgat gtagctacat taactggtgc tgctttaata
     2761 ttgttaggta atgtcgcaac tcctatagta acaaataatg atgattttta ttgtgaatta
     2821 gaaaaagctg ccactttatc aggggaaagg gtatggaaga tgccaattta tgatgaattt
     2881 aaagatatga taaaaggaga agaagcagac cttaaaaaca ctggtggtaa aaatgctgga
     2941 tgtataacag caggagcttt tattggtgaa tttgttggga atactccttg gatacatatg
     3001 gatatagcgg ggacaagtac atcatcaaaa tcaattggat acaaagcaaa gggggctaca
     3061 ggagaacctg ttagaacatt gtactatttg gcagaaatct aaaaaacaat tataaaatat
     3121 aatttataaa gccacgtgga tatttttgta atttaagatt atctatgtgg ctttattttt
     3181 tatttaaata aattatttac tctttttctt tttttcaagt ataaaagtat ctctattaaa
     3241 taaaagagca ggtatgtaca taatactagt aacaaaaata aacgtaaaaa tagcagttgt
     3301 gctatgactt aaaaaaccat aatagtacct agagttggta aatgtgttta gtaaaaatat
     3361 gaatgaaaaa taaataaaac atatagctaa gttatctcta agtgcatatc tgttaactct
     3421 aaattttcta acatcaacca tatatatatc tgcataaatt atcaagcact tagagaagat
     3481 aaagataaaa tatattaagc taggaaattc atagcccaag tttttagaaa aaatgagttc
     3541 tccaactgaa caaactaaac tccaagaaaa aaatatatta aaaagatgat actgcttaaa
     3601 aattagtatg gctatacaca gatatataca aatataattg attccaattg gtaaagagtt
     3661 tagagtacta taattacctg aagaaataag taataattgc tctagagcca taattattac
     3721 aagtaatgta caaattatct tctcaactgt atagctataa ggtaaaatat ttttggttag
     3781 ttttggacaa tagtataaaa ataatccaaa tataaagaga cttacaaatt gttcactaga
     3841 aaaaagaaaa attttaaaca tataacattc tctcctataa taaatttaat atgtatctat
     3901 ataaatatat tcattatatc tcaatttaat atataaaaaa gataataata gttagatttt
     3961 ttatatgaat tttaataggt aaaatatcct attgttttgt agatatatcc taattatgtc
     4021 aaaaaataaa aaaattttta tttattagag tttttggaaa taataataac agatattata
     4081 attaattaac agtttttgta agtatttgac aatagagagg gtgtaacaaa tgggaataat
     4141 agagtcagca tcaaaattgg ctgagatggt tcatttattg gctgttgaaa aggggataac
     4201 tgatatagaa gcatgggatg aagctgtgaa agaatacagc aaaatttatg aagaaagaag
     4261 aaatgaatag aatgataaaa aatgaaaact tgatgataaa aaataacttt atttaaagaa
     4321 attcaatttt tatagaagta ttaaaaataa tataaataaa aagatggtat ttcaaatgta
     4381 agtaaaaaat cattttggga taccatcctt attatttatt ttaataatat ataagtaata
     4441 aatcttactt atccatattt tttatgtaac ctcttttttc taactttttt atatgctcct
     4501 tacgtatttc atcaagatta ataccatatt gttcttctag cttaaacatc atagtaacac
     4561 atgtctgagc tacatccatt agttccttag cagttttctt tataatatca acttcatcta
     4621 aatcattatt ttctccactt agatttctga atttacctat acattcagct aattcacctt
     4681 gttcttcacc cattttgata aatgtagatt ctagtgttgg gtctaaatta ttaagctctg
     4741 gtaaagatat agtttttagt ttcataaaag ctcctttcaa gttattcgta tttatactaa
     4801 aacatcataa caaaaataat actaaaaaaa aactttaaat tcaacataaa aacttacata
     4861 tataataaaa atcgtgaaat aaatctatat ttgtcttaat acagcctcat ataaaacttc
     4921 tccaataggt ttatttatta caagagaagc tgacttatca tagactgtat ttgctttatt
     4981 aataagagca atataatcac ctttgtaata atctataaaa cttgctgctg ggtacacagc
     5041 taatgatgta cctcctataa tcaataaatc agcattactt atagcagaaa ttgttttagt
     5101 aatgacatca ctgtcaagag cttcctcata aagtactaca tctggtttta ctatagaacc
     5161 acagttgtca cagtaaggaa tattacctcc cagatttaac atagattcta agtcaaaaaa
     5221 cttaccacat ttagtacaat aatttctatg tacagaacca tgaagttcta gcacattttt
     5281 acttccagcc atttgatgaa gtccatcaat attttgagtt atgacagctt taagcttacc
     5341 catttcttcg agttttgcta aagctatatg agcattattg ggttttgcat taggatatat
     5401 taatttatcc ttataaaatt caaaaaattc ttctggatat ctaacaaaaa aagtatgtga
     5461 aactaattgt tcagcagtaa attgtttatt gagtttttgg ctaaataatc cagtagaact
     5521 tctaaagtct ggaatattag attcagttga aacacctgct ccaccaaaaa atacaatatt
     5581 attgtgatta gctattaagt cttttaagct atttgcatcc atagttacac ctccaaatta
     5641 ttatatataa attgtactac atattaggtc gtttatgttt tattaattta aataaatata
     5701 tagtttaata agttaaataa agggaatata aataaaatgt atacagagta taaatagaca
     5761 atatttacta aaataacaag ttgtcgtata attatattaa aattgtttta gtgtataaga
     5821 agggcggggg aagtacgtga agaaaaaagc agctttagca acattggcta tgttaccctt
     5881 aggtgtggta aatgcacatg ctgatggaga tataggtata gtgactataa attatttaaa
     5941 tgtaagaaat gaaccaactg ccgaaagtag catagccttt gttgctaaaa aagatgataa
     6001 agttttgatt aaagactctt ctaatggatg gtataaaata aaagctgaat ctggacaaga
     6061 aggttgggct tcatcaaaat atatagcaaa atcaaatagt gactctttaa gaacgtctac
     6121 aaataaagaa aaacaagtta tttcaaatag tttaaatatg agaaatggag caggtacaag
     6181 ttacagagta ataactgtac taaaaaaagg tcaaaaagta gaagtaatat cagagagtaa
     6241 tggatggtct aaaatcaaat atgatggaag actaggatat gtatctagct cttatttagg
     6301 agacgtttca aactcaacta ataaatcaaa aactaaacaa gtgaatacaa cttcactaaa
     6361 tgtaaggagt ggaccaaata caagttatgg tttgttaggc aagttgccaa aaggaagtaa
     6421 agtagaagta atatcagaag gtaatggatg gtccaaaata aagtataatg gaaaagatgc
     6481 ctatgtatct agtatgtact tatcggatgt aagtcaaagt aattcagaca attctagtca
     6541 aagcaatgat aaaaagaata ctgataaggt cgtaaataca gcttctttaa atgtaagaag
     6601 tggaccaggt tctacatata gtaagttggg aaaagtttat aaaggaagta aagtaactgt
     6661 actatcagaa agtagtggat gggctaagat taattttaac aataaagaag catttgtagt
     6721 aggcaattat ttatctactt cagcagatac ttcaaataat aactcaaata gtaactctga
     6781 taacagttct aatagtaatg gcaataattc ttcatcatca ggtcaagtaa atggtatgtc
     6841 aggtataagt ggggctaaaa ttgattataa atctttgagt tatactttag aatctcatat
     6901 aagtaaacaa gttgagaaag cagcatcagg aggaaatgtg atagctccaa gtaataggaa
     6961 aagcactcca agtcctgaat ttagtacttt ttcagctcaa agaacaagct catttgtaaa
     7021 tgcaagttca agtgatatag aatattattt aaatcctaaa aattttacaa atactactaa
     7081 aggtatgatg cagtttttaa agattaacag ttatagagat ggtatttcag aatcaagtct
     7141 taactcgtat cttaatggtt tatcttctag tgtatttaaa aaccagggag cagcctttat
     7201 aaatgctgcc aagaagtata atatagatgt tgtatatcta gtatctcatg ctatgtggga
     7261 aactgcttat ggtaagtcta cacttgccca aggtcaaaca ttaacatctt ataagggaca
     7321 acctcttagt aagccagtta aagtatacaa tttttttggt ataggagcaa tagataaaag
     7381 tgctaatgtt tcaggtgcag aagcagcgta ttccaatggc tggactagtg tagaagctac
     7441 aatagatggc tctgcaaagt ggatatcaca aaattacgta aatagttcta agtacaatca
     7501 gaatactatc tacaagatga agtggaatta tgattataca tggcatcagt atgcaactga
     7561 tgtgaactgg gctaatggaa tttctggaat tatggaaaat ttaattggtc tttatggagg
     7621 aggaagtagt ttggtttttg aagttcctca atacaagtaa tacataaata aaaaatatag
     7681 actatgggta gttttcattt tatttaaatt atctatagtc tttttgtata taatttataa
     7741 tctgtaaatt taatgctaac aaaactttta tgttataata ttaattctaa gtaaatataa
     7801 gtgagtaata ggtagaaata gataaaaagt ggtaataata tatataaaga taattttgag
     7861 aaaggaggat atatatggaa agtattaagg aatatttgga taagttagaa attaataata
     7921 gtggtcttgg aaagcaactt aaagaagttt atataaatag agtagtttat tttaaggaag
     7981 ataagatagt ttatttttat ctaacttcta aagatattgt ttctcatgaa cttttagata
     8041 aatttaaaga agaattgatg tataaactcg attattttaa agatatgagg atgaagataa
     8101 gatttactgg tcttgaaaga aaatccaata aagatgttat aaaaaaatat tggaataata
     8161 tattgtatat attaaagtat ctttgtccat ctattgcagg gtggtataaa caagtagaat
     8221 ttctttgttt agaagaagaa ttaaaaataa aacttccaaa aggtatattt tatgagcgat
     8281 taatgaaaaa aaatgtaact tatgtactta aaaccgtgct tagcgaagag ttgggattag
     8341 atttaaatat aacaatagaa aaagctgttg atgaaaaggt aaataaagaa agactcataa
     8401 gaatcaatga tagagaaatg gaagaaaaaa ttagggcttt agaaattgga aaagttaata
     8461 actgtgaaga aaatgaagag gaaagttatg tcattaagtc tgatgtagat gaaaatttaa
     8521 tctatggaga taatgcaaat gctatgattg aaaatatagt tgaattaaat gcatcttctg
     8581 gaacagttgc agttgttgga gatatatttg atgttgacac taaagaactt aaaaacggaa
     8641 aaatacttat gatagcttca attacagatt atactagctc aataagctgt aaactttttt
     8701 taacagatac aaataaggat ggagtacttg aaaatgttaa aaaaggagca tacttaaaaa
     8761 tcaagggtga catagtttat gatacatatc aaagagaaat tagtatgatg attagtggga
     8821 taagaaaaga aactagaata gagagagaag ataaatcaga ggaaaaaaga gtagaactcc
     8881 atgcacatac acaaatgtct tctatggatg ctatttgttc tgttaaaaag cttgtagagc
     8941 gtgcagccaa atggggtcat tctgctattg ctataacaga ccatggtgtt gtacaaggat
     9001 ttccagatgc tatgaatgca ggtaagtcaa ataatataaa aatactttat ggagtagaag
     9061 ggtacttggt tgaagatgat tctctcatta tagaggatgc aaatgataaa gaactttcgc
     9121 agacatttgt tgtatttgac atagagacaa ctggtttttc taatacaaat gataaaataa
     9181 ctgagattgg tgctgtaaaa atagaaaact ttaagattgt agatagattt agtgagttaa
     9241 taaatcctga aaaagatatt tcttataaga tacaggaatt gacaggaata actaatgaat
     9301 taattaaaga caagccaacc attgaagaag ttcttcctaa gtttatggaa tttgttggag
     9361 atagtgtatt agtagcacat aatgcagagt ttgatactgg atttatatcg caaaaatgta
     9421 gggaacaagg cttgttatat aataacaaaa aagtagatac acttatgtta gcaagagtaa
     9481 tgttgccaaa cttaaaaaga catagattaa atgtcgttgc aaaagaactt ggtattcctc
     9541 ttttaaatca ccatagagca gtggatgatg ctgaagctac agcacttata tttaataagt
     9601 ttttgcagat gcttacagaa aaaggtgcaa aaactctaag tgatgtaaac aatatacttg
     9661 gaaagattga ttacacaaag cttagcacaa atcatataac cttgatagca aaaaattcta
     9721 taggtataaa aaatttatat aaaataattt cagatgcaca tgtaaatcat ttttttagag
     9781 caccaagaat ccttagaagt gtattaaatg aatataaaga aggattaata attggttcgg
     9841 cttgtgaagc tggggtggtt tttcaagctg taaagaaaaa cgtgtctgat gaggaaatga
     9901 aaaaaataat agatttatac gattatattg aagtaatgcc tatagataat aatagattta
     9961 tgattgataa aggagaagta aaggacgaag aagagttaag ggagttgaat aggaaattaa
    10021 ttgatacagc caaaaaattt gacaaaattc cagtagctac tggggatgtt cattttttag
    10081 acaaacatga agctgtctta agaaaagttc ttaaatattc tcaaggtttt aaagtagatg
    10141 aagaagaaac ttatttacat tttagaacaa cagatgaaat gttggaagag tttagttatc
    10201 ttggagaaga attggcatat gaagttgtag ttgaaaatag taatctgatt gccgatatgg
    10261 ttgaagatat aaaacctata ccagatgata cttatcctcc aattatagag ggctcagatg
    10321 tggagttaag agaaatgtgt tacaaaaaag ccaaacgaat ctatggagac ccaataccag
    10381 aaatagttca aaaaagactg gatagagaat taaattcaat aataagcaat gggtatgcag
    10441 ttatgtatat tatagctcaa aaactggttg caaaatctct ttcagatgga tatttagttg
    10501 gttcaagagg ctcagttggc tcatcttttg cagctactat gagtgatata acagaagtta
    10561 atcctcttcc agcacattat atttgtgaaa acgaagactg taaatattca ttcttttatg
    10621 aaataggtga atggggctca ggtgttgatt taccagacaa ggattgtcca aagtgtggaa
    10681 gaaaacttgc taaaaacggt catgatattc catttgaggt tttcttaggt tttgaaggtg
    10741 ataaagaacc agatatagac ttaaatttct caggaaatta tcagccaaca attcataaat
    10801 acacagaaga actatttgga gaggggtatg tatatagggc tgggactata ggtactgtag
    10861 cagaaaaaac agcttttgga tacgcaagaa aatatgtgga agaaaataat ataagtgtac
    10921 caaatgcaga agtacttagg ctttcaaatg gatgtactgg agtaaaaaga acctcaggac
    10981 aacatcctgg tggagttatg gttataccag actataaaga tgtatacgat tttacaccta
    11041 tacagtatcc agcaaatgat acttcttgtg gtgttataac tactcacttt gattatcatt
    11101 caataagtgg tagaatactt aaacttgata tacttggaca cgacggtcct accattataa
    11161 ggatgcttga agatatgact ggaataaata taacagaaat tcctttagat gataaagaga
    11221 ctatgtctct atttacatct acagaagcat taggtgttac accagaagaa ataaactgtc
    11281 ctataggttg ccttgctgta ccagagtttg gaactaagtt tgtacgacaa atgttacttg
    11341 acacaaaacc tactacattt gcagaacttg tacgtatatc tggtctctct catgggacag
    11401 atgtttgggt aaataatgca caagacttag ttagagaaga tatagttgga ttaaaagatg
    11461 ttatatctac acgagatgac ataatgaact atttaatatt taaaggatta cctcccaaaa
    11521 tgagctttac aataatggag agtgtaagaa aaggtaaagg tttaaaacca gaacacattg
    11581 aagaaatgaa aaaaaataat gttccagaat ggtatatagg ctcatgtaaa aagattaagt
    11641 acatgttccc aaaagcacat gctgttgctt atgtaatgac atcatttaga attgcatatt
    11701 gtaaagttca ctatccagaa gcattttatg cgacttattt tacgacaaaa gttgaggatt
    11761 ttgatgcaga tttaattgtt aaaggattag atgctataaa atcaaaaata aatgaaattg
    11821 agtcactggg aaatgatgca accacaaaag aaaaaggtat gttgaccgtt ttagaagttg
    11881 ctcttgaaat gtatgctaga ggaataaaat tattaccagt tgatatatat aaatcagatg
    11941 caactgagtt tatagttgta ggagaaaaaa cattacttcc accaatggca gcaatacaag
    12001 gtcttggagg aaatgcggct ataaacatcc aaaatgaaag aaaaaatggt gaatttatat
    12061 caaaagaaga tttgagaaag agaacaaaaa tatcaaaaac ggtaatagaa actcttacta
    12121 atcatggttc acttgagaat atgagtgaga gaaatcaatt gtcattgttt taagttagcc
    12181 caaaaagttg caaataaaag aattttatga tatcatagta tagaaaatag tatactttgc
    12241 agaaactaag agtgagacag ctgttctcac tcttttattt taaatatatt attaaaaagc
    12301 tggtttattt ttattaaata tattttattt aagattatat aaactggtca tttacaattg
    12361 aataggagga actttaatga aaaaaaattt agaagctaca attgaggaga ttgtaactaa
    12421 aataacggat gaacatggat ttgaaatggt tgatgttgaa tatgtaaaag aagcaggaga
    12481 atactactta agagtataca tagataaaga agaaggaata tctttaaatg aatgtgaatt
    12541 agttagtaga gaattaagtc caatactaga tgaaaaagac cctataaaag aaaattactt
    12601 tttagaggta tcttctccag ggcttgatag agcattaaaa aaagacagag attttgttag
    12661 atatcaaggt agagatgtag atttaaaatt atataaacca ttaaatggat gtaagcaatt
    12721 tgaaggtgaa cttgtaggat taacagaaga taataatata aaaatcattg caaatggtaa
    12781 ggaaatggaa tttaatagaa aagatgttgc tattgtcaga ttagcaataa agttttagtg
    12841 gtatattaaa tagggaggat ataaaaaatg aatcatgaat ttatggaagc attagatgaa
    12901 ttagtagttg acagaggtat agataaagaa atacttatag atactataga acaagctctt
    12961 ttaactgcat acaagaaaaa ctttggttca gcacaaaatg ttagagttga atttgataga
    13021 gacaagggag atataaaagt attttctcaa agagttgttg ttgatgaatc agatttatat
    13081 gatactttct tagaaataga gttagcagat gctagagaga taaaccctaa ttatgaatta
    13141 ggtgatatta tagaacatga agttacacct aaaaactttg gaagaattgc agctcaaaca
    13201 gcaaagcaag tagttgttca aagaataagg gaagctgaga gagaaatagt gtataatgaa
    13261 tttatagaaa aagaaaatga aatagttaca ggagaggttg ctagagcaaa taaaaatgtt
    13321 gtttatgtaa atcttggaag aatagaaggt ataatgactc aaagtgaaca aataccattt
    13381 gagaattata aagcaggtca aaaaataaaa ttctatatac ttgaagttaa aaagactaac
    13441 aaaggacctc aaatagttgt atcaagaagt catccgggtc tagtaaagag attgtttgaa
    13501 gaagaagttc ctgaaatata tgaaggaata gttcaaataa aatctgtagc aagagaagca
    13561 gggtcaagaa ctaaaatggc agtaagatct atagatgaaa aaatagaccc aattggagct
    13621 tgtgttggac caaaaggact tagagtaaaa aatatagtgg atgaacttgg cgatgagaaa
    13681 atagatataa taaaatatag tgaagaccca gcagagttta tatcagcagc acttagccca
    13741 tcaaaggttg ttagagtaga tatagatgaa gatgaaaagt ctgccttagt aatcgttcca
    13801 gattatcagt tatcgcttgc tataggtaaa gaaggtcaaa atgcaagact agcagcaaaa
    13861 ttgactaact ggaaaataga tattaagagc gaatctcaag cagaacaaga aatgttaaat
    13921 cttgaaaatt ccaaagaaat agaagctgag gatgttgaag ctgaatttat cgaccaagaa
    13981 gagttagata cttcagagga taattttagt gaagaagagt aaggagggat gattttgaaa
    14041 aaagtaaaaa aagtgcctca aagaaaatgc atagcatgtc aagatagaga ctctaaaaaa
    14101 gagcttataa gaatagttaa aaataaagaa ggaaaaatat ttttagaccc aacaggaaga
    14161 gccaatggaa gaggagcata tgtatgtaaa agctctgaat gtctaaaaaa agctataaag
    14221 agtaaagcat taaataaagc ttttaaaata gatgttcctg atgaagtcta tgataatcta
    14281 cttatggagt tagaagaata tgaaaaataa taaagaaaaa gtactttcgt tcttagggct
    14341 cgcaactcgt gcagggaaaa tagtgtctgg tgatgattca actttgcttg accttaaaaa
    14401 aggaaaagta aatttgattt taatcgcaga agatgcatca aataatacta aaaaactttt
    14461 taaagataaa tcaactttta gaaatattcc atatctattt ttttcaacaa aagaggaaat
    14521 aggatttgca ataggaaaat ctccaagagc agttgtagga ataaaagatg aaaatttttc
    14581 taaaaagata attgaactaa tagaaattta aaataaaata gggggtgatc ttgtgtcaaa
    14641 aacaagagta taccaaatag cagaagaatt aaatatatca aatgaagagc ttataaataa
    14701 gttagctgaa ttagatataa atgtaactga caaagattca gtattagaag gtgaagagtt
    14761 agaattagca cttgaaatgt taggtgaaga tttatctcaa gaaaatggaa atgttataga
    14821 aatagatgga aagttgacag tacaagtatt agcaacgaaa ttagataaat ctccatcaga
    14881 aataattatg aagcttatga aaatgggaac tatggcaaca ataaatcaag agataagttt
    14941 tgagattgct gctttggctg ccaaagacta tggatttgaa ttaacagtag ctgaaagtga
    15001 tgatacagaa gctttagaaa tagaagcttt aatggaaatt gaagaagata aagaagagga
    15061 tttaaaacct agaccaccag ttgttacagt tatgggacat gttgaccatg gtaagacatc
    15121 tttactagat gcaataagaa aaactgatgt gatttcaggt gaagctggag gaataactca
    15181 gcatataggt gcttcagaag ttaaaatcaa tggacacaaa atagtatttt tagatactcc
    15241 aggacatgag gctttcacat caatgagagc aagaggagca caagttacag atatagcaat
    15301 attagtagtt gcagcagatg atggtataat gcctcaaaca gttgaagcta taaaccatgc
    15361 taaagcagca ggtgtacctt taatagttgc tattaataaa atagataaac caggtgcaaa
    15421 cccagataag gttaaacagg aattagcaga ccaaggatta ttagttgaag actggggtgg
    15481 agaagtaata gcagttccag tatcagctaa gaaaaaagaa ggaatagata cacttttaga
    15541 aatggtatta ttggttgctg aaatggaaga gttaagagct aatccaaata aaagagcagt
    15601 aggaactgtt atagaagctg agcttgataa aggaagaggg ccagttgcaa cagtacttgt
    15661 tcaaggtggt actttaacag taggtgaccc aatcgttgca ggtgttgcat gtggtaaagt
    15721 tagagcaatg ataaatgcaa aaggtaagag agtaaaaaca gcaggaccat ctacagcagt
    15781 cgagatatta ggtctatcag aagttccaca aggaggagac caatttgtcg aagttccaac
    15841 tgataagatt gctagaagtg tagcagcaag aagacaacaa atagttagag atgaaatgtt
    15901 aaaatcaact caaagattat cactagatgc tctatttagt caaatgagtg aaggttctat
    15961 aaaagacctt aatatagtta taaaagcaga tgtacaaggt tcagtacagg cagttaaaca
    16021 atctttagaa aaattatcaa atgaagaagt tcaagttaaa gtaatacatg gtggtgttgg
    16081 agctgtaaca gaatcagaca tattacttgc agcagcttct aacgcaataa taataggatt
    16141 caatgtaaga ccagttccag gtgcagaatc tctaggagaa aaagaaaatg ttgatataag
    16201 aacatatact ataatataca aggcaataga agatatacaa gcggctatga ctggtatgtt
    16261 agaccctgaa tatgttgatg aagaaacagg aaaagctgaa ataagagaaa tatataaaat
    16321 atctggtgtt ggaacagtag ctggatgtta tgtgactaat ggtaagatat ttagaaattg
    16381 taaagtaaga ttagttagag atagcataat aatacatgaa ggtgagcttg cagcacttaa
    16441 gagatttaaa gatgatgtaa aagaagttaa ttctggatac gaatgtggta tgagttttgt
    16501 aaattataac gacataaaag aaggcgatat agtagaagct tatataacta aagaagtaga
    16561 aagaaaatta taaattcgta tcaaagaaaa tcagttagta aagaaggtcg atggtatggc
    16621 atcatataat agaacaagaa ggatagcaga agaaataaga aaagtcgtaa gcactatgct
    16681 tataaatgga gtaaaagacc ctagaataac atctatggtg agtgtaactg atgtagaagt
    16741 tacaaatgac ctaagatatg cttatgtata tgtaagcatt ctaggtggag atgaagaatc
    16801 cacactaaca ggtcttaaaa gtgctggagg atatataaga agagaagtag gaaaaaatat
    16861 aaaacttaga tatattcctg aaatagtatt taaacttgat gattcaatag aaaaaggaat
    16921 gtatatggat agccttataa aaagagttaa tgaaaaaaat gctcaacaaa atgaggatga
    16981 aaattatgat gaataatatg gatagtatta taaactatat ttatgagggt gactatttta
    17041 tagttacctc tcatatcagt ccagatggag acaatattgg ttctacactt tcaatgtatt
    17101 atacattgaa gaatttagga aaaaatgtat attatgtact tgatgatgaa gctccattaa
    17161 atttaaggtt tttggttgaa gatgtaaaaa tctataagtc taatgaattt aatatgaaga
    17221 actattctct aattgcactt gattgtgggg ataagatgag aatatgtgtg agtgatgaaa
    17281 tcaaaaataa tgcatcaaaa ttaatctgta ttgaccatca tgctagtaat gattcatatg
    17341 gagatttaaa ttatgtagat attgatgcat cttctacatg tgaattagtt tataatttat
    17401 tagttagatt tcagcaaata aaagatatta atattataaa tgaagatata gcaacatgtt
    17461 tatatacagg tcttgtaact gatactggaa acttttcgta ttcaaatgta catgcaagta
    17521 gctttgaaat ggcaaaaaat ttacttgttt taggtgcaca aaaaaatact ataatacaaa
    17581 atatatatca aagcaattct tctggttatt acaaattatt aggagaagct ctaaagggtt
    17641 tggagatatt tgattcaaag gtatctagta tagttttaac tcaagacatg atgaatagaa
    17701 ataatattag ctttaatgat gttgatggaa taacaacata tacaagagac ataaaaggta
    17761 tagaagttgg aattttattt aaagagaaaa aacaaaatga aattaaagtg agttttaggt
    17821 caaaaaatta tgttgatgta agtgaaatag ccaagttatt tggtggtggt ggtcatgtaa
    17881 gggctgctgg atgtactatt agagatagta ttgataatgc aaagaaaatg gtactagagg
    17941 cagtgttaaa atcaatttaa ggtgacagta aaatgaataa gattataagt atattgaaac
    18001 ccacaggtat gacatctcat gatgttgtaa gtagagtgag aaagatttta aatataaaaa
    18061 aagttggtca tacaggtact cttgacccag atgcttcagg tgttttacca atctgtatag
    18121 ggaaagctac aaaagtatgt gaagttattt taaacaaaga taaatcgtat atatgtgagc
    18181 ttactttagg tataagtaca gatacttatg atgcttcagg tgaaatttta aaaaaagttg
    18241 atgattttaa atttagtaat gaagacatag aaagagcttt tgacacacaa agaggagaga
    18301 taaatcaact tcctccaatt tattcagctt taaaggtaaa tggaaagaga atgtgtgatt
    18361 tagtaagaag tggtagacaa tctgaaatta ctttaaaaac aagaagagta aatataaaag
    18421 acataaaaat actaagtata aaaggtaata aagtaatgtt ttatgttgag tgttctaaag
    18481 ggacttatgt gagaagtatt tgccatgata taggagaata tctaggttgt ggagctcata
    18541 tgtcgttttt gaatagaaca tcttctggta aatttgactt agataattca ataacattag
    18601 aagaattaga attattttat gaaaataaaa ctttagacaa atacttatat gatatagatt
    18661 atgtccttga ttcgtttaat tatgttgtat taaaccctaa tgctataaaa tattatagta
    18721 atggtggctc aatagatgat aaaaggtttt taaagaataa tttcgataaa gatgatgaat
    18781 ttgtaagagt atacagtaca gataatttct taggattagg aaagttatca aaacacaata
    18841 atacaataag tgttaaaagt gataagatgt tcatataacc aatgtattta aaaaaacaac
    18901 aaaagagata gtactataat aacttctata gtctatctct tttacttatg ctataatagt
    18961 ttgtgaataa gttaagagta aattatattt tagcggggaa catttattat ggtgattata
    19021 aaatctataa aagaaatagc aaacataaaa gaaagtgtca ttaccatagg aaattttgat
    19081 ggtgtacaca aaggacatca agttcttata ggaaaaacag ttgaacatgc aaaaaaagaa
    19141 aatatacaga gtattgtatt tacatttgca aatcatcctg taaattactt tagaccaaac
    19201 tcaacaaaaa atattataag caatgatgat aaaataaaat tattagaaga tttgggaata
    19261 gatatagttg taatcatacc atttgatgag tatatgacta aaatacctgc aaaggatttc
    19321 gtagatgaga tactagtaaa aaaattaaaa gctaaaaaga tagtaattgg acatgacttt
    19381 acatttgcaa gggcaaaaga aggcaatgtt aatctattaa aaagcttaga acatgaattt
    19441 ggttttgagg tagaagtaat aaaacccatc aaaatcaatg atgtaagagt gagtagtact
    19501 tatattagaa gtttagtatc ccagggtgat atggctaatg tcaaggaata tttgggtaga
    19561 aattacaagt tagaaggttg tgttatacat tctaaacacc ttggaagaac tataggattc
    19621 ccaacggcaa atattgacct taagaataat atgctcgttc caaagagagg tatttatgct
    19681 tctattgtac atattggaga tgagacttat tttggtgcta caaatgtagg ctataaccca
    19741 acagttaatg gaaaaacttt gtctattgaa acaaatattt tagagtttga tagagatatt
    19801 tatggggaaa atataattgt tgaatttttg gaaagaataa gagatgaaag aaagtttaat
    19861 tcaatagatg acttaaaaaa tcaattgtat aatgatacaa attatgtgta tgaaaactat
    19921 gtttgcaaaa acaagtaatg tatgataaaa tgattaagga aaataaccat tactcggcat
    19981 ttgaatactc caatcaatgc ttagtaatag gcgtttctaa aaaaatacgg aggtaatcta
    20041 aatgataaac aaatcagaaa taataaaaga atatggaaga gctgaagggg atactggttc
    20101 tccagaagta caaatagctt tattaacagc tagaataaac gagttaaatg gacatttaaa
    20161 aactcataag aaagaccacc attcaagaag aggactatta aaaatggttg gtagaagaag
    20221 aaacttatta gcttacttaa aggaaaaaga tcttgaagga tatagagcat taatagctaa
    20281 attaggatta agaaagtaat ttcattcttt ataaaaaagc aggtatagtg tatacctgct
    20341 tttttatagt taattcatta taaagtaaaa tagttaataa taaaagtagt ttatatgtta
    20401 taaaaaatta taaaatgaat aatattgggt aagaatatta aaatagtata caaatacata
    20461 atcaatttaa aaggaaagac aggataaata tagaaatttt ataacaggat aattagttgg
    20521 aataaggagg atattatttt gaaaataaag ttagtatgcg atagtttgtg cgatattcca
    20581 gatgaaatat cggaaaaaga ttatatagag atagttccac ttacagttat ttttaatgat
    20641 agggaatata tagaaggagt agatataaag aaagaagagt tttataaaaa agtaaaagaa
    20701 atcaagcaaa tacctaaaac ttcacaagct acatatatgg agtttaaaga agtgtttgat
    20761 aagttcataa cagaaggtta tcatataatc tgtatgacag gggctgcaaa cgcatcagga
    20821 acttttcaaa gtgctatgat agctaaaaat gatgtaaatg aaaatgaaaa aatacatata
    20881 tttgatacta gaaatttatc tctaggtagt ggacaatatg ttataaaggc atgtgaactt
    20941 cttgaagaag gactgggatt tgaagaaatt atagatgaat tagaaaatac tcgcagtagt
    21001 gtaagattgc tttttgcacc atatacactt gattttttaa agcagagtgg aagagttcca
    21061 gttgcaactg ctttgatagg aaatatgtta aatataaagc ctatattttt ctttgataat
    21121 ggggaagcta agttggtaaa taaggttaga ggaataaaga atatagcaag taaattggtc
    21181 gatataattt tagaaatgaa tgaagggtct ttagaaggaa aaatcgtcac aataggttgt
    21241 ggagataatt tacacgattg tgaaatatta aaagatgaag taaataaaag aataaaagca
    21301 agaagagtat tatttactcg aggaggagtt tctatttgct ctcatacagg accagatata
    21361 ttggctataa gttgttctaa ttagtagtaa tataaggttt ttgcatatat atgtgaagat
    21421 aattttatat tgttaattta tagtattttt gataatatag taaatagagt gatatacgtg
    21481 ttttttaata agaggaggac aagcattaat gtttgaacat aaaatattta aaatggattt
    21541 tgcaggaaga gaactttctg tagaaatagg aaagatttgt gaaatggcta gtggtagttg
    21601 tatagttcga tacagtgata gtatggtaat ggtaaataca actaaatcag ccaaacctag
    21661 agacggaata gacttttttc ctttaagtgt ggattatgaa gaaaaattat actctgtagg
    21721 taagatacct ggaggttttt taaagagaga aggcaagcct tcagagaaag ctatacttac
    21781 atcaagattg atagatagac caataagacc tttattccca aaaggattta gaaatgatgt
    21841 tcaggtagtt gcaacagttc tatcagtaga ccaagattgt acaccagata tagtagcaat
    21901 gataggttct tcaatagccc tttctatatc agacatacca tttaatggac caacaggttc
    21961 agtatgtgtt ggccttgtag atggtgcatt tgttgtaaat ccaaacgcag agcaaagaga
    22021 aaaaagttca atgcatctag ttgtttcagg tacaaaggaa gcaataatga tggttgaagc
    22081 cggagctgat gaagttccag atgaggtaat gcttgatgct attttatttg ctcaccaaga
    22141 aataaaaaaa atagttgagt ttatagaagg tatagttgca gaagttggaa aagaaaaaat
    22201 gcctgttgaa ttataccatg caggagaaga aataactcaa ttggttcgcg aatttgcaac
    22261 tgataagatg aaaaaagcag ttcagacttt tgaaaagtta gagagaatgg aaaatatgga
    22321 tagagttaaa gaagaaactt tagctcactt tgaagaaact ttagaagatt ttgaagattt
    22381 tgttggagat atagaagaag tattacaaga tataataaaa gaagaagtaa gaaaacttat
    22441 agtacatgaa aatgtaagac cagataatag aaaacttgaa gaaataagac ctatatggtg
    22501 tgaaactggc atgataccta gagctcatgg ttcagccata tttactagag gacaaactca
    22561 agtacttaat gttgctactt taggtgcttt aggagatgtt caaaaactag atgggttaga
    22621 tgaagaagaa aacaagagat atatgcatca ttataatttc cctgcatata gtgttggaga
    22681 agctagacca tcaagaggtc caggaagaag agaaatagga catggtgctt tagcagaaag
    22741 agctttatta ccagtaatac catctcaaga agaatttcct tatgctatta gattagtttc
    22801 tgaagtatta agttcaaatg gttctacatc tcaagcaagt gtatgtggtt caacattatc
    22861 tttacttgat gcaggtgtac ctataaaaga tatggttgca ggtatagcaa tgggacttat
    22921 aaagcatgat ggtaaggttg cagtgctttc agatatacaa ggtatggaag accatttagg
    22981 cgatatggac tttaaagtag ctggaacaga gtatggtata acagcaatac aaatggatat
    23041 aaaaattgat ggtatagata aagaaattct acaacgtgct ttaaaacaag ctaaagaggg
    23101 tagaatccat atactaggag agatgagaaa aactatatct cagccaaaac cagaactttc
    23161 accatatgca cctaagattg ttaaaatgca aataaatcct gacaagataa aagatgtaat
    23221 aggacctggt ggaaagataa taactaagat aatagacgaa actggagtta agatagatat
    23281 tgaacaaact ggggaagtat ttatatcagg tattgagatt gatatgatta agaaagctca
    23341 agaacttata aataatatag tagttgaacc agaagtagga aaaacttata aaggtaaggt
    23401 aagtagaata atgaactttg gagcatttgt tgagatactt ccaggaaaag aaggattact
    23461 tcatatatct catatagctc atgaaagagt tgccaaagtt gaagatgtat tgaatatagg
    23521 ggatgaagtt gaagttaaag ttacagagat tgatgaaaaa ggaagagtta atttatcaag
    23581 aaaagttctt ttaccaaaac cagaacataa aaataaataa cttattatat aattaaaagc
    23641 aaagtcttag gattttgctt ttttaaatac aaaaaagtag ataattttaa aatgatagtt
    23701 atggatggct tatagttctt tttctgtcag tctttactct tataaagaag ctatttaaaa
    23761 gctgattaat agaaagcttt attgatatat atagaatcac ttaagttata tttagtgcat
    23821 aaacagagct tatttttaat agtattaaat aagaaacaat ttgtacataa ttagggggag
    23881 ttttaatgat aatgatggta cttaataaga gtaagctcaa aaaaatcata atactaattt
    23941 tagtagtgtt attagtatta tcaggagggg taatggtatt aaagttattt aacaacaagc
    24001 cagtatttaa tttatttaat ggtgtagatt taccatatga aagaggtaca aaggataaaa
    24061 gtggatatgt tgcattaacc tgtaatatag accttggatg ggaaacagag tacgtagaat
    24121 ccattttaga aacacttaaa aaagaaaatg taaagattac atttaatgta actggaaaat
    24181 gggcagaaaa gaataaggat gaattattaa agataaaaaa acaaggacat gaaataggta
    24241 atcatggata taaacattta gattattcta cattatcata tgaagacaat tatgagcaaa
    24301 tagagacctc taaaaagata attgaagaaa ttataggtga aaaaactaaa ttttttcaag
    24361 caccagcagg ttcctttggt ccagaaacag taaaggctgc taaagcatta gggtatactt
    24421 ctatcaaatg ggatgccgat actatagatt ggaaatacaa agaccaacca gaggtaatca
    24481 ttgatagaat gaagaaaaaa gacattaaag atagtagtat aatcttaatg catccaacca
    24541 acgcaacgac aaaatgtata gatgatatta tagctattgt aagagaaaaa ggactaaaag
    24601 caggaaaact tagtgatgta tttaagtaga caaagtataa atatttggtt aagattgtat
    24661 aagttgtagt tgaattaatt aaaagactga cttataatgt aatacatata aaaaataaat
    24721 ataattaaaa catagtgttt tcaataatcc aatatgaaac aaagaataaa ttgttaggag
    24781 aatgtaatgt ataagacaaa aatactggaa aatgggctta caataattgg tgaagaaata
    24841 ccatatctaa agtcaataac attaggtatt tggataaatg caggctctag gatagaagaa
    24901 gcacaggtga gtggaacttc tcattttata gagcatatga tgtttaaagg aaccaaaaac
    24961 agaacatcaa aagagattgc tagttcaata gataatttgg gtggtcaaat aaatgcattt
    25021 acaagtaaag aatgtacatg ttattatgta aaactaatag atgagcatat agatacagga
    25081 attgatgtat tgagtgatat gatactaaat tctaaatttg ataaaaatga tatagataag
    25141 gaaagactta taatattgga agaacttaag atgtatgaag actctccaga tgacttatct
    25201 tatgatttat tagtggaaaa tatatatgct aatgatggat taggaatgaa tataattggg
    25261 acaaaagaat ctctatacaa tataactaga gaatccatgt tagaatactt aaataagtat
    25321 tatattccaa ataacgctgt tatttctata gctggaaact ttaactttga tgatatggtg
    25381 gagaaaatca aatcaaaatt tggacactgg gaaaagaaaa atttgagtat agacataagt
    25441 gaggctaagt ttaatccttg ctttatatca aaaaataaag atactgaaca ggtgaactta
    25501 gctatgtgcc taaaaggtat tccttttgaa aatgatgaag aagtctattc aatggcagtg
    25561 gtaaataata ttttcggtgg aagtataagc tcaaggcttt ttcagaaaat aagggaagaa
    25621 aaaggtttag tatattcaat atactcatca cagacattat ataggaaatg tggtgaatta
    25681 ggtatatttg caagcatgag tacagaaaat ttacaagatg tatataattt aataaagaaa
    25741 gaaattgaaa atataagaga aaactatctt acagaaaaag aaatatctga aagcaaggaa
    25801 cagttaaaag gaaattatat attggattta gaaagtacta gcagtagaat gatgtctact
    25861 ggtaaatcta tgttacttag taaaaaagta aaaactacag atgaaatact tgaatgtata
    25921 aatagtgtta atattaattc aataaagaag gttgttgata aggtattcaa tatcgaaaat
    25981 attggtacat gtatagttgg aagagatgta gaaaagattc tacacttgga ttaatattga
    26041 aaaaagtaaa tctctggagg tataaaatat gaatttatct gaaatagcag gtaaggaaat
    26101 agttaacttg gtcacaggag aaagattggg ggtcattgga gaatgtgacc ttatactaga
    26161 tgaaactaca ggcggtatat tagcgcttct aattccaaaa gaaaaaggat tttttgccag
    26221 aagaaaagat aagtcatttt tagaagtacc atggagaaat gtaaaaaaaa taggtaatga
    26281 catgataatt atagaatatg agggatatat ttaggaggaa aaaatgagta ttttagtgca
    26341 aaagtttggt gggacatcag tagaatcata tgaaaaaatg aatgaagtat gtaagatagt
    26401 aaaagcatac aaaaaaaatg atgaagaatt acaattagta ttagtagttt cagcaatggg
    26461 aagaaaagga gctccatatg ctacagatac acttataaat ttgtgtagtg cagtaaatga
    26521 tgagccaagt aaaagagaac ttgatttaat aatgtcttgt ggagaaataa tatcaggaac
    26581 aattcttgct aatttattga atgcacaggg gatagattct gttttcttta ctggtcagca
    26641 agcaggtata attacaagtg atgaatattc taatgcaaag ataaagtata taaatcctaa
    26701 aaaggtaaaa agagctttag atgatggtaa ggttgtagtt atagctgggt tccagggtgt
    26761 aacagatgat ggagaaataa ctactcttgg aagaggcgga agcgatacat cagcagttgc
    26821 tattggtaaa gctttagaat gtgaaacagt tgaaatttac actgatgtgg atggtataat
    26881 gactgcagac cctagagtag aaccaaacgc taaagtattg agttttatag actatgagga
    26941 agtattccaa atggctgaca aaggtgcaaa agtaatacat ccaagagcag tacagttggc
    27001 taaaagtgga aatataactt tagccataaa gaatacaatg aatccaactt ttgaaggtac
    27061 aaaaataggt tctttatgta gacacttaga agacaatata gaatatgaac aagaaagaga
    27121 ttttaaggtt gctgttgcaa ataaagatag cgtagcacaa gttaagatta agtcagcaga
    27181 agaagtgttt acagaagtat taaatgagat agaaaagaaa cttataacta tagatatgat
    27241 aaatttcttt atatcagaaa aagcatttgt tgtagaagat gctgatataa aatcattaaa
    27301 ggaaatacta gataaatttg aattagatta tgaagtaaat agagactgtg ctaaggtaac
    27361 attgatatgt tcaagaattg ctgatgaaat gtctggtatc atgtcaaagg tagttagagg
    27421 attatcaaaa gcaggggtat cccttttaca aacatcagac tcaaatatga ctatatcttg
    27481 tttagttagt gaagaagata tgcatacagc agtacatgct atacatcaac aattttattt
    27541 aaaataaact gtataataca attatttttt aatcaaattt aagaatttca tatatatctt
    27601 aatatatttg atttatttgg taaaactaaa ttaaatataa ttttgatagg agttttttta
    27661 tgaaagatta tagagaagat aatgaaagat ttgataaaga taattgtagt gaagaatcta
    27721 gttgcgaaga gagagaatgt ataaagcaat ttggtaccaa tgaaatgcca cctcaacctc
    27781 ctaaagatat tcaatgtata actataattg gagaaattga gggacatttt ataggaaatc
    27841 ctcaaaaaaa agctacaaag tacgagcata taattccaat gctatactca attgaggaaa
    27901 gtaatgatgt taaaggagtg ttagttgttt taaacactgt tggaggagat attgaagcag
    27961 gtttagctat tgcagaactt ttaaacagta catctaaaaa agtagttact cttgttctag
    28021 gaggaagtca tagtataggt gttcctcttg ctacagcagg agattactca tttatagcac
    28081 caacagcaac catgattata catccagtgc gaactactgg tcttgtgatt gggataaatg
    28141 agacttttga gtattttaaa aagatgcaag atagaataat tcaatttata ataagaacat
    28201 caaatatcaa aaaagatgta cttgaaaaac ttatgcatga aaaagatgaa cttgtaagtg
    28261 atgttggtag tgtattaata ggaaaagaag ctgtggatta tggtcttata gatgaagttg
    28321 gaggactaaa agaagcactt aaaaaattaa gagagcttat aaaagaatct gaggaagaaa
    28381 aaaataatga ttaaaatttg tttaaataat aaaatttatg atataatgat gtaataaagt
    28441 aggagtgtta gtgttaaggc attagcactt tttgctgtga aggggtaaaa cttgctaaaa
    28501 ataaggagtg atattaatgg ccacgaaaaa gaagaaaaaa aagaaggtaa ataccttaag
    28561 ttttaatgca gaatatcata atttaataac aatatttata ggtgtatttt tattatatag
    28621 tttaaattct aattctatgg gatggatacc tgtgttaatg caaaatcttt ttaagggact
    28681 atttggaggg ctttcaatag caataccatt tatagtaatt ataacaggac ttttaggatt
    28741 ttttgatgga aatgaatata tatataggct aagaaagact aaactatact acataattat
    28801 tttatttata tttgtatttt atggattatt aaatgcagga actcttcctg tagatagccc
    28861 actaaaaggt aacatgtttg atgatgttat gaaattgggt gtaagtggac aaggtagtgg
    28921 tttgatagca acaactattg cgtattatat gagtaaaata tttggtatag caggtggatg
    28981 gttaataagt atcttcgcac ttatactttc tgtaatgttt atatttaata tttctataaa
    29041 agatttggtg tcaaatgcaa aatcaaaatc taaagcatct aaagacagca atttaacgtt
    29101 taaagataaa attgcaaata tgaaaaaatc tgcaatagat atgatgactg atgaagtaga
    29161 tgatactact ataaataaaa aaccaggttt ctttaaaggg ttaatgtcaa aaggtcttaa
    29221 taatgatgat gaagaagatg aatattttga agaagaaaat agtgatggtg tagatgataa
    29281 aactataaaa atagttggat ttaataaggc agaagatgag tatttagaaa tattagaagg
    29341 aactcaaagt atgccagagt tagatgtttt aaaagaatta caaaaagcta ccaatgaaaa
    29401 tccagttgta gatactaaac cagaaaaaaa agttgatata gctaaaccta atttaaatat
    29461 agaaaaaact caacctatga gtattgtagc agagccagtt aatgaggatt attcaaacta
    29521 taaaaagcct agcattgagc ttttaaataa agtaaataag aagtctgatg aaaatggtaa
    29581 aaagaaagtg ttgaaaaatg cttctctttt agaaaagaca ctatcagatt ttggagtgga
    29641 agcaaaaatt aatcaagtta cagttggacc tactataaca agatatgaaa tacaaccaag
    29701 tccaggagtc aaggtaagta aaattgtaaa tttaactgat gatatagctt taagtttagc
    29761 agctaaaagt ataagaattg aagccccaat tcctggtaaa agtgcaattg gaatagaagt
    29821 tccaaatgaa gaagctcaaa tggttggtgt gagagaagtt ctagaaagtg aagaatttaa
    29881 taattttaac tctcctcttg caatgggact tggaaaagac gtagcaggaa aaattataat
    29941 tggagatata ggtaagatgc cacacttact aatagctggt tctacaggtt caggaaagag
    30001 tgtgtgtgta aatacattaa taagtagtat tttatataag gcaaatcctg atgaagtaaa
    30061 attactatta atagacccaa aagtagttga acttgcaaat tataatggaa taccacatct
    30121 attgatacca gtagttactg accctaaaaa agctgcaaat gctttaaatt gggcagttac
    30181 agagatgaat agaaggtaca aattatttgc agatgctcaa gtaaaagatg taacaagtta
    30241 taatgagaaa gcggaagaaa aacttcctaa aatagtaata ataatagatg agttagcaga
    30301 tttaatgatg gcaagtgcaa atgatgtaga agattatatt tgtagattag ctcaaatggc
    30361 aagagctgct ggaatgcact tgattgtagc aactcaaaga ccatctgttg acgttataac
    30421 aggggtaata aaggctaata taccatctag aatagcattt gcagtatcat ctcaaactga
    30481 ttcaagaaca atccttgata tgggtggtgc agagaagctt ctaggtaaag gtgatatgtt
    30541 attctatcca cttggtgcag caaaaccagt tagacttcaa ggggcattca tatcagagag
    30601 tgagtcggaa aaagtaatcg attttgtaaa aagtcaagtt aaagatggaa taaaatatga
    30661 agaagatata attgaaacta tatctaaggt taatacttca aagggtagtg atgaggatga
    30721 gtttttgagt gaagcgatag aatttgtcgt ggaaagtggt caagcatctg cctctatgct
    30781 acagagaaga tttaaaatag gattcaatag agcagctaga cttatagatt ctatggaaga
    30841 aagaggaata attggagcaa gtgagggaag taaaccgaga aaggtattaa taagcaaaca
    30901 ggatttgcag aatttagaag gtgaatagaa ttgtcagtta taaaaataga aaaagcactt
    30961 aagctagata aagtaatatt tgtagatgtg agaactgaag gagaatatga agaagaccat
    31021 atattgaatg caatcaatat gcctcttttt aaaaataatg aacataatga cgttggaaca
    31081 atttataaga tgcagggaaa acatgaagca atacaaaaag ggtttgacta tgtatcgtat
    31141 aaattaaagg atatatattt acaagcagca gaacttgcat taaattatga taatattgtc
    31201 atatattgtg caagaggcgg aatgagaagt ggcagtatag taaatttatt atcatcatta
    31261 ggtgtaaatg tatatcaact tgaaggtgga tataaggctt atagaaattt tgtattagag
    31321 tatctaaggc atgtaatgga tacaaaaaac tttgtatctg ttcatggatt gactggtaca
    31381 ggaaagactg atttgttaca tttattagat gaaaaaggaa tagatgtact ggatttagaa
    31441 ggcatagcta aaaacagtgg ttctaccttt ggttttataa cttttgataa aaagccacct
    31501 tctcaaaaga actttgaaac aaaactattt gaaagtatat ttttttcgaa agagaattat
    31561 atatttgtcg agagtgaaag caaacgagtt ggaagtgtta cagttccaaa tgaaattttc
    31621 gaggcaatga cacgagaagg acatcatata ttattagaat gtgatattga aaatagagtt
    31681 gatagacttt gtagagatta tatttatgat aaagatgaag gtaatatatt gattttaaaa
    31741 gaatgtataa acaagtttag aaaaagattg ggtaataaag tagtagatga ttatattgat
    31801 ttattagaat caggtgaata taaagaactt gtaaaaagat atcttttaga atactacgac
    31861 ccactttata tgcattcagt agaaaaatat aagtatagta agattataaa ttttaatgat
    31921 acacaaaaag ctttagaaga tgttatagac ttatatgaga gtatattgga aggagaaagt
    31981 gaatgttaaa aatagcatta gaatcattag gatgctcaaa aaatttagta gatgcagaaa
    32041 taatgatggg aattttaaat aacaaaggct ataaattaat tggagatttt gaagaagctg
    32101 atgtaattat agtaaataca tgtggattta tagagtcagc taaacaagaa tctatagata
    32161 cgataataaa ctttgcagag ttaaaaaaaa ctggcaattt aaagttgttg atagtaacag
    32221 ggtgtctagc ccaaagatat tctgaagaat taaaaactga aattcctgaa atagatgcta
    32281 tagtaggaac aggaagctat caaaatatag ataaaatact taaagaatta agtgaaatgc
    32341 ataaaattgt aagccttaat gatatagagt ttgtatttaa tgaagattta ccaagatata
    32401 tatctacacc tagctatatg gcctatttaa aaattggaga aggatgttcc aataattgta
    32461 cttattgtat aataccaaaa ctaagaggta aatatagaag tagaaaattt gaagatataa
    32521 ttaaagaagc taagaaatta gctgaatctg gagtaaaaga acttgtggtt atagcacagg
    32581 atacgacaaa gtacggtttt gatttatatg gaaaagaaag attatcagaa cttttagaag
    32641 aacttgcaaa aatagatgga tttaagtgga taagggttat gtattcatat ccagaatcta
    32701 taacagaaga attgatacaa gttataaaga aatatgataa tatatgtagt tactttgata
    32761 tgccaataca gcatgcaagt aacaatattt taaaattaat gaatagaaaa actactaaag
    32821 aagatatatt aaataaaatc aatctgataa gaagtaatat accagatgct atacttagaa
    32881 ctacaataat agtagggttt cctggtgaaa cagaagatga cttcaaacaa cttgtggact
    32941 ttgttgaaga ggttaaattt gatagacttg gtgcatttgc atattcaaga gaagaagata
    33001 caccagcaga tagattgcca aaccatatag atgaagaagt aaaaatacaa agaagagata
    33061 ctttaatgat gattcaacaa aaaatatctg aagaacttaa tgacaaaaag ataggaaaaa
    33121 cttatgaggt gttaatagaa gaacagatag aagataatgt atatacagga agaacacaag
    33181 gtgatgcaga ggaaattgac agtatagtgt atgtaaaaag tgtagacaat cttgaagtag
    33241 gagaatttgt aagtgttcaa ataaatgatg caatggaata cgatttaatg ggagatgttt
    33301 tatatgaact tgcctaacaa gttaactttg tttagaatat ttttaatacc agtatttgtt
    33361 ttaattatgt tgctcaatat accaaataaa tttttaatag cttgtattat ttttataata
    33421 gcttcaatta ctgatgcttt agatggaaaa atagctagaa aatacaatct agtcactgac
    33481 tttggaaaat ttatggaccc attagcagat aagttattgg ttatttcagc acttacatgt
    33541 atgatagaag accatcttgt aagtagttgg atggtaatta taatagtggc tagagagctt
    33601 acagttagca tattaagagc tatagctgct gctgatggaa aagtaattgc tgctggaaac
    33661 agtggaaaac ttaaaacgat aactcaaatg gtatctatag tgtttttatt gttaggtgct
    33721 cagtttgaaa atgtattgat attaaatatt ggtgaaattc tcattcttat agctacacta
    33781 cttacattat attctggatg ggaatatcta tataaaaata aagagctttt tatgtcttct
    33841 aagtaaaaaa ttatgcttta ataaataaaa actaatgatt aataaataaa aaataacaac
    33901 ttaaaatttt aaaaatgtaa aaaagggtat actattatag tatacctctt ttcattttaa
    33961 gaatattttc ttttgtgtat ataattatta ataatggttt ataaataaaa tgtaatgaat
    34021 aaaaatataa tcattagtag cgtttttaaa ttgactaggt ttgatttatg ttatataatt
    34081 aatatataac aagagaacaa atgtttgtag ataaatgaag ggatggtata gagatgagtg
    34141 tagatcaaga aaaattaaaa gcgttgaatg aagctttagg taaaattgaa aaagattttg
    34201 gtaaaggttc agtaatgaaa ttgggagaag caacatctat gtctatagat gttatatcaa
    34261 caggagcgat tggtttagac atagctattg gtataggagg tctacctaga gggagaatag
    34321 ttgaagtata tggtccagaa tcttctggta agactactgt tgcgcttagt tgtgtagcat
    34381 cagctcaaaa agatggagga atagctgcat ttatagatgc agaacatgca cttgacccag
    34441 tatatgcaaa agctttgggt gtggatgttg ataacctaat aatatctcaa ccagatacag
    34501 gtgaacaggc tttagagata gcagaggcat tgataagaag tggagcgata gatataatag
    34561 taatagactc agtagcagca ttagttccaa aggctgaaat agatggagat atgggtgatt
    34621 ctcatgtagg tttacaagct agacttatgt cacaagcact tagaaagtta actggttcaa
    34681 ttaaaaaatc aaattgtgtt gctatattta taaaccagtt aagagagaaa gtaggaataa
    34741 tgtttggaaa cccagaaact actactggag gacgtgcact aaaattctat tcatcagtta
    34801 gattggatgt tagaaaaata gatacaataa aacaaggtga taaagttata ggtagtagaa
    34861 ctagagttaa agttgttaaa aacaaagtag caccaccatt taagcaagct gaatttgata
    34921 taatgtatgg agaagggatt tcaaaaattg gagacctttt agatatagct gctgatgtag
    34981 atatagttaa aaaatcaggt tcatggtata gttacaatga tactaaactt ggacaaggaa
    35041 gagaaaatgt taaaaaattc ttggaagata atttagattt aactactgaa atagatgaga
    35101 aagttagagc attttacaat ttaaatgaag aacatgaaga atcaggtact tcagtatcaa
    35161 aagaaattgt agaagaataa gtaaaaatac aaaaaaataa ctagtaatta aagacatgcc
    35221 acgctcccta ttagcttgac acaatgttga aaaaattata aaattaagtg aggataaaat
    35281 atctaaattt aattttaatt attaggaata ttttttaatc atagtgatga cattttggga
    35341 ttattaacga aggaggtgga tgtcatagat agtgtagtaa tgattgtaat aggtgcagct
    35401 gttggtatta tagctggata ttttgttaga aaaaatatat ctgaagcaaa aattggacaa
    35461 gctgaaaact tggccaaaga aataatagat aaagcacacc acgactcaga aacagtacaa
    35521 aaagaaaaat tactagaagc taaagaagag atccataagt ggagaacaga agctgaaaga
    35581 gaaaacagag aaagaagaac agaagtacaa aagtatgaaa aaagagtagt acaaaaagaa
    35641 gaagtcttag atagaaaatt acaaaattta gaatctaaag aagtaaacct aagtgaaaaa
    35701 ttaaaggtag tggaaaagaa agaagaagaa gttgaagtaa taaaaactca acagttagaa
    35761 aaattagaaa gtatatcagg aattacttct gataaggcca aagaaatcat cttaaccaat
    35821 gcggagagag atgtaagacg tgaaatgtct ataatgataa aagagataga aagtcaagct
    35881 aaagaagaag ctgataagaa atcaagagaa attataggat atgcaattca aaaatgtgca
    35941 gcagatcacg tagccgaaac tactgtaaca gtagtaagct tgcctaatga tgaaatgaaa
    36001 ggtagaataa taggtaggga aggtagaaat ataaggactt tagaaacctt aacaggtata
    36061 gaccttataa tagatgacac tccagaagcc gtaattttat caggatttga tccaataaga
    36121 agagaaattg caagaattgc tctagaaaaa ttaatagcag atggaagaat acatccagct
    36181 agaattgaag aaatggttga aaaggcgaga aaagaagttg acaatattat aaaagaatat
    36241 ggtgaacaag ctgcatttga aactagtgta catgggattc atccggaatt aataagatta
    36301 ttaggtagac ttaactatag aactagttat ggacaaaatg tacttaaaca ttctatagaa
    36361 gttgctcata tagctggtat aatggctgct gaaataggag cagatattag attggctaag
    36421 agggcaggtt tattacatga tataggtaag gctgtagacc atgaaatgga aggtacacat
    36481 gttgaaatag gtatggattt acttagacgt tataaagagt ctaaagaagt tatacatgcc
    36541 atgagtactc atcatggaga ttacgagcct caaacagtag aggctgtatt agttacagca
    36601 gcagatgcaa tatcagctgc tagacctggt gcgagaagag aaactttaga agcatatata
    36661 aagagacttg aaaaattaga agaaatagct aattcatatg aaggtgtaga aaaatcattt
    36721 gctatacaag ctggtagaga aataagaata atggtaaaac cagaagctat aaatgatgaa
    36781 gaaatacatc tattggctag agatatgact aagaaaatag aggatgaact tgaatatcct
    36841 ggacaaataa aagtaagcat aataagagaa acaagagcta tagagtatgc gaagtaaaag
    36901 aaataatatc taaataataa gaaaatattt ttagaaattt aaatttgcca caagatatga
    36961 taatatatct tgtggctttt aaattgttta agtttaattt tatataatgc aaaaataatc
    37021 taagtgctaa aagtgctact tacaaaagat taataaaata aagctttata tatgtattat
    37081 ttatataatt atccaagttt gtttgatata atgaagttgt aaaataaata tattactact
    37141 tggaatgctt gtacttaaat aagatgaaaa tacaatgacg ctaaagtgag gattagagag
    37201 agggtacact atgaaaaaaa atacactttt acatacaaat attatggttt gcattattat
    37261 tactcttggt tttgtggcta cctctataat tggatatcaa tctaatacca atatttttaa
    37321 aaatgatatt gaacatgtat ctacattggc tgctgaaggt atttattatc agatagataa
    37381 actactttca gaacctataa atgtatctct tacaatggca aatgacagtc tattaaaaaa
    37441 ttttttagat ggtgagaaag aacatttaaa tgatgaagaa tttatttata aattacaaga
    37501 ttatctgaat gtgtatagaa ataaatattc atatgattct gtttttttag tatcaactaa
    37561 aaccaataat tattatcatt ttaatggact tgatagaaat cttagcgcta ataactcaga
    37621 gaatcaatgg tattatactt tcttaaagaa tgatgatgaa tactcattaa atgttgataa
    37681 tgatgaagca agtaacaata gtattacagt ctttgtaaat tgtaagataa aagatgataa
    37741 tggagcaact atgggaatta taggtgttgg gttaaaggtt aattcattac aaatgttgct
    37801 taaaggatat aatgacaaat ttgatgttgt tgcacgctta attgatgata aagggtttgt
    37861 acaattagca gtagataaaa ccggtcatga gaatataaat ttttttgaaa attctagtag
    37921 cgacttgtct aattcgaaac aattaatatt aaacaacaaa aaggaacaaa agagtttttg
    37981 gtattcatct gaaaaatcaa aaagttatat tgtatgtcag tacataccta gtttaaaatg
    38041 gcatcttgtt ctagaaaatg attttacttt aatgattaaa cagcttcatc tgcaattttt
    38101 taaaggcatt gcagttatag ttttaattat aatatttgta ctttttacta ttacacatgt
    38161 gcttaaaaaa tacaaaaacc aaataataaa gcttagtgtt tcacagaaat tggaatatca
    38221 aaaattgttg agcaaatcta ctgaaggtct ttatgaaaat atttttgaaa ttgatattac
    38281 taacaataaa gctggttgtg aagatgagaa taggtatttt catgaatttg gattagggct
    38341 taatacccca tatgatgaag ccttacatac aatagctaaa aaccaaatca aagaagaata
    38401 tgcacaaggt tatttagata catttttacc tgaaaatgta attaaacgtt ataatagcgg
    38461 aattaataat atcagctatg attttttaat taaaaaagat gaagaaagtt atcattggat
    38521 tagaattaat gcacgaattt tcttttggac atcagatgaa tctgttcgta tgatatctta
    38581 tcgtaaaaat atagatgaag aaaaaaatag agaattacta cttctcgaac gttcacaacg
    38641 tgattctctt acagggcttt acaataaagc tacaacagag gaatttattc gttcattttt
    38701 aaaaaatgaa agtaatcaag atttagaaca cgtttttcta attttcgata ttgataattt
    38761 taaaaatgta aatgataaat atggtcatgt atttggagac tttgttataa gtgaatttgc
    38821 cttagaatta aaatctcaat ttcgtgagga cgatattgtg ggacgtattg gtggagatga
    38881 gtttgtagta ttaataaaag actttaataa taataactat gtgcttatag acaaattaga
    38941 gcgtttttgt tctaagatta atcagaaaaa atttgttgag caagcagaat tttctattgc
    39001 atgtagtatt ggtgtagcaa tgtatcctga acatggatat gtatactctg aactatatga
    39061 gaaagcagac caagcacttt actatgcaaa aagccatggg aaaaattctt atcatatatt
    39121 ttcagaggat tctattgata gtatttcatt tcatatagac tctagagata taaaagtatt
    39181 attagaaatg gctactgatg gtgttgtcaa atttgcttgt atggaaaact taaaattact
    39241 ttatttcaac caaaaaatgc ttgaatttac tggatattca gctcaaactg ttacttctat
    39301 aggttttgat attttttcgc ctgtattaca agacgactta caaatggtac tagatacatt
    39361 aagagaagca atttctaaaa gagagacttt tgaaatagtt tttcgttttt gtcatacaga
    39421 tggtcatatt tttcctgtaa ggatgcaagg taagtttgta agtgaattat atcaaaatcg
    39481 ttatcctgtt ttttatgcga tttatacaga catatcaagt atcaatactt tactagaaaa
    39541 taaatagtaa atactacagt aaaatctaaa aataggatat aatagtatta atataagaag
    39601 attataaata aaacctgcta atgaaaatag attattttta gcaggttttt aaatttttta
    39661 taataaatct tatttttggc taacaagatt tatttttttt aagactttac ttattatttt
    39721 aagcattaat tatgaaattt atcattttat tatttacaga aagtatttta taacaataaa
    39781 tatctatact tattgcaaaa tttttttagc agttttgtat ttatttgtat ttattttgaa
    39841 tttatttact aaatttgcct ataaaaagta tatataaaaa tatagttcaa aatggtataa
    39901 ttaagtataa cagaaggagg taatgtaagt gagccaatac ataaatataa attctcagtt
    39961 agacgcccta aaattgaagg agttcgagaa aatattttac gaaaatatca atgaggacgt
    40021 taaagtagtg ccaaaagtta ctccttttaa aggaataaat accgatttgt tgtatgttaa
    40081 agatggaaga attttattta taaaatttat ggatacaaca gaagatatat tttttatttt
    40141 agaagaagag ttacttgaag ttatgaatga ggaatatgaa cttttgaaac taaaaatggg
    40201 acaaaagaat agaaatataa gttataacta cgtttacatt atgccatacg ttgaagttga
    40261 ggaaacatat gaatttgaag aatttgttaa taataatata atagataaaa ataagcttca
    40321 agatataatg aataagggtt cgctggagga atatcttaat gatgagaatg atgaaataaa
    40381 cttaaattta tttttactag acatatgttc agaatactat ataataaatg ataaacttca
    40441 tctaaatgaa aaatttaaaa aaatttcatt ctacaatgat gactataagt atacagcaac
    40501 tatgatggaa gacgttcaaa ttaaagatgt tgtttctatt aaatatggga atacacttat
    40561 tgaaggtggt tctggtatag gcaaaacagc aattatgctt tctagggcta taaaattagc
    40621 aaaagtatat ccacatcata aattagtcat atttacacat acaaaacaac tgtgcaatga
    40681 gcttcgagag aggatagagt tattatacaa agataataac aatttagaag ttcacacatt
    40741 tagttctttt atatttaaac tagctaaaaa attcaattta attgtagatt ataacatgtt
    40801 aaaaaatgat tacgaaaaga cctttaataa tcttgtaaaa caagcacaga acattataaa
    40861 aaataaaaat atgttcaagg ctatttttat tgatgaagca gaaagctttt tagagtatga
    40921 aatagatttt ataagggagt ttttatataa aacaaaattc atatttaatg tgtgttcttg
    40981 taactccttg aatatctcta atagattgaa tatatttaaa aaattatata atggtataga
    41041 atttgatgat aagattatct taagtaaaaa ttatagacaa gctaaagaga ttgtagattt
    41101 tacaaataaa ttttcaaata attctaactc atatatcaac gaattaagac caaatacaga
    41161 atttagtact ttcttttata ctaaagcact tagaggtgga aataagtctg ttgatataat
    41221 aaaagtaagt gaccttgatg accaaataag ttctgtaatc tgggaaattg agtatttaat
    41281 aagtaaaaaa ggtcttgatt attctgaagt tgctatcgtt tatccttaca ataagaagaa
    41341 attaaaaagt ggaaaaacca tttactttca atatatgtta aaaaaagcat tggaagaggc
    41401 aaaaattcct tatatatgtg cagaagataa tttgacaaat ataagtaaga aggtaggaac
    41461 tacaatagca aatatatatg caataaagaa tttagagtat aaagcggtaa tagtatgtga
    41521 attagagatg ttatacaatc aaactataaa tgatattgaa caagattatc aagtaaatga
    41581 ctttgtagga gatttaaata aggtttattt agctatgagt agagcaacag actacctaag
    41641 tatagtaaca acttttaatg aagaagcaag tgatataata agacttatta cagaatcaaa
    41701 agatatttag taaaaaaata tttgtaattt aagaaaataa aaagagactg agatttgtaa
    41761 aataaacttt accaattcag tctcttattt tttagtctac tttcccaata ttatcaagag
    41821 gtaatagtct caccataacc ttaccaagaa cttcatcttc atcaaccata cctacatcag
    41881 ggaatctact atcattacta ttttctctat tatctcccat tgcaaatagt ttaccttttg
    41941 gaacaacagt atctatatct ccagaagtat agttattgtg tatatatggt tcatttaata
    42001 attttccatt tacatacact ttagaatttg atatttttat tctgtcacct tcagtagcta
    42061 taactctttt tattaagtct tttttctttc ctgtttctcc atcaactaaa ttggttttaa
    42121 aaactacaat atcgcctttt tcaggtttac caaccttgta tgatatccta tttaaaatca
    42181 gataatcttt atcatctaaa gtaggataca ttgattctcc acttacaata gaaggtctta
    42241 taaattgtag aattacaaat gcaagtacaa tagccatagc tattgactta atccaatcaa
    42301 atatttcttt tttaacactc ataacttttc tccttatcat ttgttattaa attagaatac
    42361 attataatag aaagaaataa gcaagaacca ctatacccaa gactattcta taataaccaa
    42421 atactttgaa gtcatgtttt ttgatatagt ccataaaaaa tttaataact acaattgaaa
    42481 caacaaatgc tactacagaa ccaacaccta aaataagcca ttcaaatcca gtaaatccaa
    42541 atccagcttt tagaagtttt aatgcactag ctccaagcat tgtagggatg gctaagaaga
    42601 atgaaaactc tgctgctaca tatctagaag taccaagtaa tactgcacct attatagtag
    42661 aaccagacct agatgtacct ggtataagag ctagacactg gaataatcct atacaaagag
    42721 ctaatttata tgtaacttga gaaaaatctt tatacttagg ttttttattt ctcttttcaa
    42781 gccctatcat tattatacca tatacaataa gagcaatagc aactactgtt gaattaaaga
    42841 ataatctatc tatatcatct tcaaacagta ttccaataac tccagatggt ataactgcaa
    42901 ttataacctt aagccataaa cggactgttt cttttttctg cttaatattc tttgcgctat
    42961 caaaaggatt taattttcta aaaaatataa caagtacagc taatattgaa ccaaattgaa
    43021 ttacaactaa aaatgtactt ataaatgtat ttgaaaaatt caattttata aactcatcaa
    43081 ctaatatcat atgccctgta ctacttactg gtagccattc tgttattccc tgaactatgc
    43141 ccaaaatgac tgccttaaaa atttcaagca aactaaatgt ttccaaatca aaatacctcc
    43201 aatacaaatt tatagtagca tattttattc atagattaat aaaaaaatgc caccttttac
    43261 taacttataa taccatagta ctgtgtagtg cacaatggaa gattgatatt ttcataaaac
    43321 cttcataata taacaaaata aacttaaaag ttatctattg aataaggggt atatttgagg
    43381 gggaaatata tggtatagta aaagtatagc tattttattt tatgaaaagg aaaggagtta
    43441 ttatgtatag tacaaaaata ctagatgatt atgagtatga aacttttaat gttttttcta
    43501 caaaattaaa tccaaacacg aaacacgatt atttatcaaa ggttatactg tttaaagaat
    43561 ttttaaaagg gaaagaattg atttatgcta cgaaagaaga ttgtaaaaat tttgtagact
    43621 atatacagac taaatatgcg aaatcaactt gtgaaaagat atatagttat ttacatagtt
    43681 tttataactt cttaaaaaaa gaaggataca tagatataaa tccatttaga tatgtagaaa
    43741 aacccacagt aacaagaata aaaacaaaag atgatgttct aagtatacaa gaaataaata
    43801 aacttatagg tatcctacca aaattaaata taagagatag agtaataata gtctgtctag
    43861 ttactactgg gtgtttatta aatgaattag ttagcttaaa gtggaaagac cttatggtag
    43921 atgaaaacga taactcatat gtaaggttag gtaagggaag aaaagaaaga gtagttaaat
    43981 tacatccata cttttttaaa cttttagaag attacaggaa ttattctggg cttccagagg
    44041 taataatacc tagtgatgat tttattttta caactcaaaa gagtaattct ataacagata
    44101 gaaatgtcag acttatagtc aaaaaagcat tagatttagc tggactttct caatattcag
    44161 caagagattt tagacattca tttgcagcaa taagtttaag gcttggggca gatgaaagtg
    44221 atgtaaaaaa tcagcttgga tggagtgata aatactatgc aattagatat aagtatgtat
    44281 taaattttgt tgatagtgaa atagtggatt acttaattga aaaagataat ttggatataa
    44341 ataaaaaata gtaataaata aaaaatacta tatataaatg tgaacaaaaa ataaaaaagt
    44401 cttgttatag ttcgtaaaat tatatataat tatttatgta ctaatgtgta cacagtaatc
    44461 acacgacaaa agatttaagg aaggtgctaa tataatgata gataataaat atactactta
    44521 ctcaaaccca cttacagaca gatattgtag caaggacatg agttatatat tttctccaca
    44581 atttaaattc tctacatgga gaaggttatg ggttgcactt gcagaaggtg aacatgagtt
    44641 aggaatcaat ataactgaag aacaattaga tgagctaaga gctaatatag ataatataaa
    44701 ttttgatgaa gctagaaaaa tagaaaaaga agtaagacat gatgtaatgt ctcacgtaaa
    44761 agcttatggt ttacaatgtg aaaaagccaa aggaataata catttaggag caacatctgc
    44821 atatgttgga gataatacag atgttataca aatgaatgaa gctttaaaac ttctaagagt
    44881 aaagcttgta aatttaataa acaatttaaa agaatttgca cttaagtaca aagatatgcc
    44941 agctcttgga tttacacact tccaagctgc tcaacttaca acagtaggaa aaagagcttg
    45001 tttatgggct caagatttaa ttcttgattt ggaagattta aactatagaa tagacaacat
    45061 gaagttaaga ggagttaaag gtactactgg tactcaagca agttttttaa gcttatttga
    45121 aggagaccac gaaaaagtaa aagagttaga taagaaaatt tgtaaaaaga tgggatacca
    45181 ttcttcctat gcagtaagtg gacagacata tactagaaag ttagattatc aagtaataag
    45241 catactatca gggatagctc aaagtatgca taagatgact aatgatataa gacttttaca
    45301 aagtttaaaa gaattagaag agccatttga aaaaaatcaa ataggttcat cagcaatggc
    45361 ttataagagg aatccaatga gaagtgagag aatagcatct ttatctaagt atataatctc
    45421 tgaatctata agccctgcaa tggtacaagc cactcaatgg ttagagagaa cattagatga
    45481 ctcagccaat aagagattgg caatacctca agcatttatg gcagcagatg ctatacttga
    45541 aatagggata aatgtaactg atggtttagt agtatatgaa aatatgataa atagacgtgt
    45601 aaatgaagaa cttcctttta tggctacaga aacaatactt atggaagctg taaaaagagg
    45661 tggagataga caggaattgc atgaaataat aagagaatat tctatgaaag ctgcctatag
    45721 agtaaagcat gaaggcaagg ataataactt aattgaactt ataatgaatg atgattcatt
    45781 taaaatgagc aaggaagaaa tactatctat aatggaccca aaaaacttta taggtagagc
    45841 tccagaacaa gttgtagaat ttgtaaatga agttgtagaa cctgctataa aagattataa
    45901 ggaagactta ggaaaagtag atgtagacct aagagtatag aattgtacag ataaaatata
    45961 attaaaaacg gctatcttat ttataaataa gatagccatt ttaatgggct agcgtcttct
    46021 ctccaccgct gtgaagagtt cttctttact tactatacca tgaatttaat aaaatagcta
    46081 tataaataat atttaatagg aataatataa ataaaaataa aaaaatgata agattactaa
    46141 ttaaatttaa acattcagca aaaactattg tacttgcaat atttttaatg taaactgaaa
    46201 taaagttatt tagataattt aataaatttt aaaacaaccg aggtgattat atgaactata
    46261 tagtaaaaga taccataagt tttttagtaa aaacatttcc taagatttat tctagcctgt
    46321 acttagaaga cctaaaaaaa tttgcaccag attataatgt aaataaaaca caattaagag
    46381 cattagtttt cataaaaaat tatggagtaa taagtatgac tgatttatgc tctaagttaa
    46441 atatagaaaa aggcagttta actagtatgg ttgatgattt aactgataag aagtatgtaa
    46501 caaggaaaag agatttagtt gatagaagaa aatatctaat tgacattact gaagagggag
    46561 ataagatagc aactgatttt atggataaat taagtgatgg tttaggagaa aaactttcta
    46621 aattaactga agaagacaga aaaaaatatt tagaagcaat aaatactctt caatatatat
    46681 taaacaaaga agaatttaga tagaaaaaat ataaaaattt ttgtatttaa tggtactaag
    46741 attaattctt tgttattcat ttaactatat aattataagt ctaaatacaa tctaaaaagt
    46801 acatcttaaa ttttcttgat gtactttttt ataatttctc taactaaggc aagacaagtt
    46861 ttagatgctt taattaaagt gaataaagat tactggccaa cactatataa agaaaatgaa
    46921 aaatagtcag gtctaagtct taattaatat attaattaag aaatgtcccc ttttcaaata
    46981 atatctgaaa aggggacatt ttatatcatt aaatttatat aaattaagtt taaaaaatta
    47041 tttttgtaaa gctacatatt gctctaaacc ttcttttaat aatgtagcag ctctatgaag
    47101 gtctttttca tttaagatgt aagctaatct tatttcatca cgtcctaaac ctggagtagc
    47161 atagaatcct tcagcagggc aaaccataac tgtttcatta tctttattaa attcttttaa
    47221 catccaaatt gtgaaatctt ctgcattttc aactggtaat ttagcaacta tatagaaagc
    47281 accagttggt tttttacata taacaccttc aaccttcatt aattcattgt ataatacatc
    47341 tcttcttttt ttgtactctt cattaacttc tttgaagtaa gaaacaggag ttttgtataa
    47401 ctcaacagct cctatttgct ctaatgtagg aacacaaagt cttccttgac ataatttaag
    47461 tatttgagcg ataagacctt tatttttgca agcgatagaa ccaattctag caccacaagc
    47521 actgtatctc ttagaaacac tgtctattat tataactcta tcctgtacac cctctaagtt
    47581 accacagctt gtatactcaa gcccatcata aacaaattct ctataaactt cgtctgctat
    47641 tatccataaa tctttttctt tagctatttc agctaggata ttaagttctt cttttgtata
    47701 aattgcacca gttggatttc ctgggttaga aagaattata gccttagttt tatcatctac
    47761 tttagacaat atttcttctt tagatggaag gtggaaaccg ttttctgcct ttgttgtaac
    47821 agcattaact tctacgttta cagattgacc aaatccattg tagtttgtat agaatggttc
    47881 tggaactaat agattatctc ctgggtcaca aactgccatc atagtgaaaa gtaaagcttc
    47941 actaccaccg ttagttacta ataattcatc tttttcaaaa ttcatatcgt aagttttata
    48001 gtaattttga agtgcttcta ataattctgg aataccttct gatgtagcat attctaagac
    48061 ttcgctatca aagtttttaa cagcatcaaa aaatccttta ggagttttta tgtctggttg
    48121 tcctatgttt aagtgatata ctttgattcc tttatctttt gctgcttgtg caaatggaac
    48181 aagttttctg attggagagg cttgcattgc actaactctg tttgaataat tcattttaat
    48241 accccctatt taatttaaat acgtatattg tattatgtaa attgtatgtt tgaatacaag
    48301 acatctttac aagataataa taacatactt atgggtaaaa atcatcaaaa aaaattaata
    48361 tagctttaaa ataaactttt aacaaattat agtaaaatac ccatgtttat gccactaaat
    48421 tatgtattca taaattaatt tatagtaaga taatattctg atgtactgat agtgattttt
    48481 tagttataat ctttcagcta tagacaaaat atataataat aaatatgcaa aattataaaa
    48541 aatattataa atttatttaa cagatacgat aaatatcata taattatatt aaaggctatt
    48601 aactaagagg tgttaattat gaaagaatta agaatagatc caattaccaa tgatgttgtt
    48661 atatttgcta cagataggtt aaaaagacct ttagatacag cagatatccc aaatgaagat
    48721 gaagaaacta atgaatatga tgaagaatgc ccattttgca aaggaaatga agctcatgca
    48781 actgatgcat tatttgaaat agatagtgaa gaaggatggc ttgtaaaatc tgtctacaat
    48841 aaatttccta taattgatga tatggcaaga gatgtatatg gtgttcatga agtaatgata
    48901 gagagcgata aacataatcg tagtttttat aacatgagtc aaaaagaatt tgaagatgtt
    48961 ttttttatgt atagaaatag atttagagat ttgtcgaaag atgataaagt agagtatgtt
    49021 agtatcttca aaaacttctt gagaaaggca ggagcatctt tgatgcatcc acatgctcaa
    49081 atattgtcta tgtcttttat tccacctgaa attacgaatg aactgttggt ttcaaaggag
    49141 tattatgaca gcaataaaag ctctttgtat gatgatttaa tagaaaatga aataagttta
    49201 gatagaagag ttgtttataa tggagaagct tttttggtac ttataccata tgcaaccaag
    49261 tatagtggag aagtaagaat aatatttaaa gataaaatag agtttggtga gttaaatgat
    49321 aataatatac aggagctttc tacaatattt gaaaagttgt ttaagaagtt atacaatatc
    49381 aatggatata tgccatttaa tttatgtatt cacactcatc caacaaagat tgaaacaaaa
    49441 tcgtatttta atgttcatat gcacataatc ccaagaaaat ataattttgg aggatttgaa
    49501 cttggaacaa atatgtatgt ttcttcaatg agccctgaag atttaacaaa aaagttaaaa
    49561 attgattaaa acatatattt ttaggtatta agtaaatagt aattttagat gaaattatag
    49621 tatagcattg ttaaaataaa agtaaattaa gaatttacaa taaaattgtt gaaaagcccc
    49681 catctttagc tatggggagt gtgtcaatgt ttagatgagg agtataagta tgaaatttat
    49741 atcatggaat gttaatggaa taagagcttg tgttgggaaa ggatttttag actttttcaa
    49801 agaagtagat gcagatatat tttgtttaca agaaactaaa ttgcaagaag ggcaaataga
    49861 gttagattta ccaggatatt ttcaatattg gaattatgct gagagaaagg gttattcagg
    49921 tactgctata tttacaaaaa aagagccttt aaaagtaatg tatggaataa atatagaaga
    49981 acatgacaaa gagggtagag tgataactct agagtttgaa gatttttact ttgttacagt
    50041 atatacgcca aattctcaat ctgaattaaa gagactagaa tatagaacta gatgggaaga
    50101 cgactttata gattatttaa ccaaactaga taatcataag cctgtaattg tgtgtggaga
    50161 tatgaacgtt gcacataaag aaatagattt aaaaaatcct aaaaataata tgaaaaatgc
    50221 aggttttact aaagaagaaa gagagaagtt ttctaaatta ttagacagtg ggtttataga
    50281 cacatataga tattttaatc ctgataaaga aggtgtttat agttggtggt cgtatagatt
    50341 taatgcaaga aaaaataatg cagggtggag aatagattat ttttgtgcat ccaaaaaatt
    50401 agaagataga ttagtaagtg cggatataca cactgaaata ttaggttcag accattgtcc
    50461 agttgaatta gagataaaat aaaaattaat tatgataaaa attgggctgt agatataaat
    50521 ttaaagttaa ctagtttttt aatctacatg cctatttttt atctaaataa actctatttg
    50581 ttttttcttt gcttaacatt ttctaatgta aattgatttg ttttccacat attataagct
    50641 aagttaatga tttttttctc gtctttagtt aattcacgat ttaaaatttt cctaaattca
    50701 tcaaataact tatcataatc aactcttttt ttgttagaat atcttatagt tttaggtttt
    50761 atactcgtat aaagatatat atctgggact atacttggat tgtatatctt aataaaaact
    50821 ttagcagtat aatatgcatc atttaaagca ttgtgataag acattttttc atcaagttcc
    50881 aatatagaaa tagcattttg taggccaatg ctttttcctg ctggattatt gaaatattta
    50941 gatgcatgtt gttgtatatt aatataagat ttaggtaagg aatttgatgg tagcttgtga
    51001 tagttgatat tcctgtataa ttccttcaaa tctccagctc cccaaacaca taatataggg
    51061 tcttgtgatg atataaattt tataaaattg ttgtagacct cagggaaagt aggcgaatca
    51121 ttaaaatcag aacttttaat attagtcata cgactaataa agggatgtat gtctttataa
    51181 attgtaggtt ttacatagct attaaacgta tctattatat tgaatttaga atccaattta
    51241 attgctccta tttgtataat ttcaaaagga cacttttcat tggatacagt tttattgagt
    51301 tttttatcaa atccttggtt aaattccaaa tcgaaaatta tgtattccat aaagtttatc
    51361 tcctaaattt ttttattatt tcttttacta tagattatat tataaataaa attaaat
//
LOCUS       DAYASV010000014        48324 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYASV010000014 DAYASV010000000
VERSION     DAYASV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.014"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(148..759)
                     /locus_tag="ACGI68_000840"
     CDS             complement(148..759)
                     /locus_tag="ACGI68_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0137288.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
     gene            complement(851..1840)
                     /locus_tag="ACGI68_000841"
     CDS             complement(851..1840)
                     /locus_tag="ACGI68_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFH0137289.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            complement(1907..2770)
                     /locus_tag="ACGI68_000842"
     CDS             complement(1907..2770)
                     /locus_tag="ACGI68_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HFH0137290.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            complement(2876..3472)
                     /locus_tag="ACGI68_000843"
     CDS             complement(2876..3472)
                     /locus_tag="ACGI68_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HFH0137291.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGASGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            complement(3512..4315)
                     /locus_tag="ACGI68_000844"
     CDS             complement(3512..4315)
                     /locus_tag="ACGI68_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFH0137292.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDRTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            complement(4351..4791)
                     /locus_tag="ACGI68_000845"
     CDS             complement(4351..4791)
                     /locus_tag="ACGI68_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0137293.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            complement(4804..6906)
                     /locus_tag="ACGI68_000846"
     CDS             complement(4804..6906)
                     /locus_tag="ACGI68_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0137294.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKSTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            complement(7167..8870)
                     /locus_tag="ACGI68_000847"
     CDS             complement(7167..8870)
                     /locus_tag="ACGI68_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFH0137295.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            complement(8896..9855)
                     /locus_tag="ACGI68_000848"
     CDS             complement(8896..9855)
                     /locus_tag="ACGI68_000848"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0137296.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            complement(9991..10509)
                     /locus_tag="ACGI68_000849"
     CDS             complement(9991..10509)
                     /locus_tag="ACGI68_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFH0137297.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            complement(10552..11037)
                     /locus_tag="ACGI68_000850"
     CDS             complement(10552..11037)
                     /locus_tag="ACGI68_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HFH0137298.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            complement(11034..11681)
                     /locus_tag="ACGI68_000851"
     CDS             complement(11034..11681)
                     /locus_tag="ACGI68_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HFH0137299.1"
                     /translation="MNAIIKGGVYIINYLELDDVHIDALREIGNIGSGNAITALASMI
                     NSNVEVLIPMVKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLML
                     SSLFNKEEAFLDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVG
                     AVLNVPMIRFSDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            complement(11716..12078)
                     /locus_tag="ACGI68_000852"
     CDS             complement(11716..12078)
                     /locus_tag="ACGI68_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0137300.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            complement(12822..13319)
                     /locus_tag="ACGI68_000853"
     CDS             complement(12822..13319)
                     /locus_tag="ACGI68_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0137301.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEIHI
                     YIGIK"
     gene            complement(13494..14405)
                     /locus_tag="ACGI68_000854"
     CDS             complement(13494..14405)
                     /locus_tag="ACGI68_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137302.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            15329..16180
                     /locus_tag="ACGI68_000855"
     CDS             15329..16180
                     /locus_tag="ACGI68_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFH0137303.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            16184..16654
                     /locus_tag="ACGI68_000856"
     CDS             16184..16654
                     /locus_tag="ACGI68_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137304.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            16667..17977
                     /locus_tag="ACGI68_000857"
     CDS             16667..17977
                     /locus_tag="ACGI68_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0137305.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            18189..18935
                     /locus_tag="ACGI68_000858"
     CDS             18189..18935
                     /locus_tag="ACGI68_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0137306.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTDDSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            19297..20838
                     /locus_tag="ACGI68_000859"
     CDS             19297..20838
                     /locus_tag="ACGI68_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0137307.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKASISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            20873..21979
                     /locus_tag="ACGI68_000860"
     CDS             20873..21979
                     /locus_tag="ACGI68_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0137308.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFQGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGEIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(22358..23644)
                     /locus_tag="ACGI68_000861"
     CDS             complement(22358..23644)
                     /locus_tag="ACGI68_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0137309.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(23767..26865)
                     /locus_tag="ACGI68_000862"
     CDS             complement(23767..26865)
                     /locus_tag="ACGI68_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0137310.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRKKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKHLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVTLDCVVIQGYYYCKPIPSADFEKLLY
                     QK"
     gene            27591..27749
                     /locus_tag="ACGI68_000863"
     CDS             27591..27749
                     /locus_tag="ACGI68_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137311.1"
                     /translation="MKIFSFLLFVIGICLCIAGFIGHYPVFVGTTLSFLAIAMIAVRQ
                     VKTKKKMK"
     gene            complement(28036..28740)
                     /locus_tag="ACGI68_000864"
     CDS             complement(28036..28740)
                     /locus_tag="ACGI68_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137312.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(28731..30338)
                     /locus_tag="ACGI68_000865"
     CDS             complement(28731..30338)
                     /locus_tag="ACGI68_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HFH0137313.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(30592..31770)
                     /locus_tag="ACGI68_000866"
     CDS             complement(30592..31770)
                     /locus_tag="ACGI68_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137314.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVAAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(31996..32490)
                     /locus_tag="ACGI68_000867"
     CDS             complement(31996..32490)
                     /locus_tag="ACGI68_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0137315.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNIGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(32491..33252)
                     /locus_tag="ACGI68_000868"
     CDS             complement(32491..33252)
                     /locus_tag="ACGI68_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0137316.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            33954..36788
                     /locus_tag="ACGI68_000869"
     CDS             33954..36788
                     /locus_tag="ACGI68_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0137317.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIMHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            36831..38075
                     /locus_tag="ACGI68_000870"
     CDS             36831..38075
                     /locus_tag="ACGI68_000870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0137318.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
     gene            complement(38490..40232)
                     /locus_tag="ACGI68_000871"
     CDS             complement(38490..40232)
                     /locus_tag="ACGI68_000871"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137319.1"
                     /translation="MKKTINNNLFRILNIIVYKNKTKLIVSFIMVFIIAGVNIVIPQL
                     IRCIIDDGIGNKNIFLLINLILIYIFIRILSATFDVILSYLYTSIKNKVTINLKVKLL
                     NHISKLSGRYYSSKKTGNILSIMESDIFMIENFGAEIIFDIIVNVFTAIISLIFLINL
                     QFDLFLVVLFLQLILTFSQNKMTKLISKKISEIRSDSGSLSNLIQEYVSNIMDIVILN
                     SKKNFFNKYLKRERDLIKKDLNLNIIMSSNIAISQILTGLITILIYGIGGYKIINNKM
                     TMGELIAFQQYTGMLIGPCIAIVRANTRIAQSKVSINRVFSVIDEKIDIKVDNNGYRI
                     DNSEIKKIDFKNVSFRYGEDLNLVLNDINLQFEKGEVTGIVGGSGCGKSTITNLIYRL
                     WDINEGDILIDGVNIKDINLKSLRNNIDIVTQDQFVFDDTIKNNIVLNNKISDKELDE
                     ILEVTCLDDFIKNLDKGLDEVVGEKGVKISGGQKQRISIARALVSNKKVLILDEATSA
                     LDNISQKEILNNLKEYIQDKIVIIIAHRLSTIKSADNIYVLDKGRVIESGNDAELLHK
                     DGLYNKLVETADFA"
     gene            complement(40216..40890)
                     /locus_tag="ACGI68_000872"
     CDS             complement(40216..40890)
                     /locus_tag="ACGI68_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137320.1"
                     /translation="MKILKVKDLKKIYGKYENEVRALDGIDLDIEPHKFTAIVGASGS
                     GKSTLLHCVAGLDKPTLGNVYLDDVDLYTLNDDKLSEIRRQEFGFIFQSYNLIPVINV
                     YDNIVLPVLLDGKKEDKEYVMRLINKLGIEKQIKKYPNELSGGQQQRVAIARALANRP
                     SVIFADEPTGNLDSKTTNEVMNMLEWCVKEYKQTLVMITHNDNIAKTADRIITIKDGK
                     IDEKNY"
     gene            complement(40927..41916)
                     /locus_tag="ACGI68_000873"
     CDS             complement(40927..41916)
                     /locus_tag="ACGI68_000873"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFH0137321.1"
                     /translation="MTKVRIAVLDTGLDVNDDDINGNYVYDSNFQLKDLGGKEINDIN
                     GHGTFVSKTILNISKNTQIYPINIFNDSGKTSSLNLLSTLSKLTMSDIQIINISAASI
                     NSMYEKELEKVCNILNSQGRFIICSKHNKYEDKESIPTIFESVIGVVGNENIYKNNQY
                     IYEPENKIQMYANSKDNFIKFKDKVTHFGKNSKACAVATGIVANILKNNPYIKFNELE
                     EILIQKSKSKEDLKIENLNTPSYKSNLIRLDIARKIINVINRNFSKIKIDFEFVSEYS
                     LFNNFTCIGNYNAYNFLTILNKEFNISIDYKEVYLYQLENLDMAVDLIYENLI"
     gene            complement(41964..43208)
                     /locus_tag="ACGI68_000874"
     CDS             complement(41964..43208)
                     /locus_tag="ACGI68_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137322.1"
                     /translation="MNSSNKYFLDCICEKIINNNQVILSNKVNGRWVKIPIECYEVLK
                     YSVDNALQLGNLYDVFQENEDKKYYERVIDKLEHIGLLSDSILSEFRLERINKISFSP
                     TNRCNLSCDYCCVDSKIDNNDYLDTSQVRKVIDNIVKLNPRILTITGGEPLMRNDFLE
                     LLDYIKEKFKGKIILSTNATLIKEKQIDDIINGLYAIEISLDGYDELSCSEVRGKGVF
                     CKVLNVIRLIKLKKFENVGLSMVVGKNNEKDIEKFNKLNEDLGTTPIIRNFMNIGRGN
                     ENYSKYLNDKLDIDYICKDDYVDESSLCSNMCKAGVNQISIDHKGDVYPCPNLEYKDL
                     KIFNILEFNENILERILNREMDIFNIFDKLKPVNIESCKECKVNLFCTTCPSKIYTLK
                     NNKKMFDANCKKMKNILEPILW"
     gene            complement(43250..45796)
                     /locus_tag="ACGI68_000875"
     CDS             complement(43250..45796)
                     /locus_tag="ACGI68_000875"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0137323.1"
                     /translation="MINIKLAVSYLKRQKGKTLSLILSIGIAVMLVFSLSVINESQGQ
                     SDINHAYKNSGDYHAFYEDINSDIVKELNGEEDIKEFNDVLKFGEIISKDNGASLNLC
                     SYNKDFLDSTRYKLVKGREPRTSNELVIEQKVLDKMELDVKLNQTIDFQIVNEHINKN
                     NENSIYSKDKQFKIVGILSKSDEYYENLNKYKVRAFTYSNNGKDIVPYELITHMGTLK
                     LNTKDLDSQKVVNLGRKYKLTSNQFYPNTEVSMSMYMKNISKKTTYSIKQSLLPLLCA
                     TLVIYNMFNIIILDMTKQIGLLRAVGASKRNIRQVFFIQSIVVLSVGIVIGLLGGIVF
                     SYLGLMIVYDKSAELTINTNSIIESIIMSIIAVILASVVPIYKAGNLSIIDAIKKTDK
                     LNYIPRLLLLKSHKKVVGIINEIAFKNIFRNKVTSIIIIITISLCGVLFIGRLTSTKF
                     IYGKESDSANITSKSYGNFDIYLGYSPINANYTFSKYDNSLLNEIKAIEDVTDVEPNI
                     YLKGYLRNADLEEDYLDELKRTEINNNNESTVLIRGYNKELLNNIDKYIDKGENVYSY
                     TDRDYKNVLLVNNFYSRIKLSNNTQIIKSPKIGDLIDIKIPVYKDGEYKYINTKVRIA
                     GIMKNSYISEQDGESQSGGIQIIFNEKDLKELTGISDYNKVFVKIKKETDKKVIKEIN
                     NIIEKSGFSDIEGRYVRNHFGDHYNKSSYKLAMICVFGVLLISSINIIFIVRSNIIVR
                     LSEICTFRAIGMSKKNVKRMLIKENMIYGVLSMIVAGIISSFNYYKIVSETNKLNQQV
                     YGINNSIKFEIPIYEILIFGSISVFVCVIAVYFSNKMINKLSIVSGIKEN"
     gene            complement(46004..46165)
                     /locus_tag="ACGI68_000876"
     CDS             complement(46004..46165)
                     /locus_tag="ACGI68_000876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137324.1"
                     /translation="MIHIKKIEEKIELQDVEVQADPCKWYYDKHGIISQIDCMVDCAG
                     STPKGTVAY"
     gene            complement(46221..47237)
                     /locus_tag="ACGI68_000877"
     CDS             complement(46221..47237)
                     /locus_tag="ACGI68_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137325.1"
                     /translation="MKFTIFVTEDCNLRCTYCYEGNDKKKENMSYETADKTIDFICNK
                     ELESNSDYTTIVLHGGEPFINFDLIKYIHRDLVDRLSDKKILFDITTNGTIINDEIIE
                     FIAKNVKNISISIDGKKNIHDINRIDANGVGTYEKVMENGKRLIDKGIYVRARMTYNS
                     NTVKYLYESVYDIAKQGFKNIAPISDFMDKNWDNSHIKILNEQISKIMNIKKDYPDID
                     ISILDKQFINKKKGDCFGGINSYAIDTRGDVYPCTVCVGDKDILLGNIYDGLDDKKIK
                     NLIKIYISKNEECDGCTRLDYCMSTRCKLINKKVTGKFQSAVPIYCGLERVYVETTKK
                     LCSL"
     gene            complement(47234..>48324)
                     /locus_tag="ACGI68_000878"
     CDS             complement(47234..>48324)
                     /locus_tag="ACGI68_000878"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137326.1"
                     /translation="INLIDIMNKNQIFKSNNLNFDMLSNGVAIYFCLTNRCNLFCKHC
                     CVESSSNKKDLLSTYDLKNIVDIISVLNTPHIIITGGEPLIREDFFEIIKYIKQKLPD
                     TKLILSTNGTLIDEHNVDFIITNFEKIDISIDGVDENTCSETRGKGVFNKVISSVNLL
                     KDNGFSNIKLSMVFGQKNSHLNKQFKDLNKKLGTEFVERYFIPTGRGLENKESYLIED
                     TVLPITIPYILDNELKMCKDKKNKKMGCCACNAGNSQIFINYDGDVYPCPSLIKDDYK
                     MGNIFDNEFVKVIKENKTRDLKSLIKLESIYPYNFEKCKDCDVNIFCWHCPAILDSVK
                     DNELELDRWCSMMKHNLNTAVWGDEKVN"
BASE COUNT        16198 a         8006 c         5287 g        18833 t
ORIGIN      
        1 aaagtaaatt atttactatt tatcaagata aattatttat aaaataaatc atttataaaa
       61 tataatttat actttgaaaa taaaataaac tatcttccaa acattttatt atatgcttaa
      121 aagatagttt tgtgtcttta aaataaatta aattgtatat attccttcac ataacttttg
      181 tatattacta ggtggttgcc atttaccatc ttttttagta tatgtaatca tagttgtaac
      241 ttccttagtt ggtttcttat tgtaaaggtc tataatcatt tttgtcatat catcttgaac
      301 ttctttttct tttttatcgt aattcttttc atcagttaca taagatttta ctaaagtgtt
      361 cgcttcactc attactttac tagtatctaa agctttactt acaactttca aagttatctt
      421 atccttacta gaagattcca ctgttactgt cttttcaaca ccttgaatta atttaacaat
      481 tgtttcgtat atcttttcag cattttcttt accataaaga ggtgtcaatt gagcaatcat
      541 tgaaaccttg tccttgctgt tataagtttg aatctgttta tattcagctt ctgttaatgc
      601 atcagaaatt cctttttcat catacttata catttctaat gcagctaaat tagcttcaaa
      661 attcgacttt actttttctt ctgcattaga acaccctata gttagaacta aaactagagc
      721 cataatcgta gataatatta acttttttac cattttcata caaacatcct ccctcaatta
      781 aaataataaa aaatttattt atcaacatta taacataaat ttgtctaact atagaattat
      841 tttgaatatt ttaccacttc cagtctattc caataccgtt ttcttttgct ttatcgtata
      901 ttctttttgc tgttacgaca tcttgtacac ctatcccaac agttttaaat acaattatct
      961 catcatcatc ttctcttcca ggaatagttc cgtttataac atttcctaaa tcaccactaa
     1021 aatcatcttt acttatctta ccttctttta atggaattaa tatatctcct gactcagata
     1081 aaactgcatc tgttgattca aaaaatatct tagacgctct agttaaacag tttgggcaca
     1141 actcttgcat atctggcata tatgaaccta ctgcacttat taaagctcct tttttcacta
     1201 agtttccatt aagtactggt tgttttgatg gagtagctaa aactatcaca tctgcgttat
     1261 ctatggcttc gtcagatgat tcaactgcaa ctattttagt attatattta cttaatttta
     1321 agttcatttc ttctgcaaat ttttctctac tttccttgtt tctactaaaa actcttacct
     1381 catccaaatt tctagcttct atcatggctt caagttgtgg ctcaccttgt cctcctagac
     1441 ctatcaaagc tccaatctta gaatcttttt tagccaatac gtctatagct gctccagagg
     1501 ctgctccagt acgaatctga gttacataag taccatccaa tactgcacta acttctccag
     1561 tttctccatc taataatagt actgttgctg gaattactgg tttgcctttt tgagcatttt
     1621 taggaaatac agatacaatt tttattcccc cacagcctaa ttcttctacg tatcctggca
     1681 taaataacat actagcatct tgacttggaa cagatatatt ggttctcaaa ggatttacac
     1741 tctttccttc acaataaatt ctaaaagctt ccttatcagc ttcaattgca tctctcattg
     1801 taaatacttt ttttatgtcg tcttttttta aaagtagcat ttttataccc ccaattttaa
     1861 taagtctcta tgctaaatat tatagcataa catttaattt aatcttctat ttcaaatcaa
     1921 ataaatctac atactctggt tttagcatag aacttatcaa cgctttattt attgaaaatt
     1981 ctggtattcc tgctgcatat ggagttatat catacaaatc aaaatataca actagattct
     2041 catcttgcaa gtaaaagttt tgattctctt ttataccttt aaaattatat attccgatat
     2101 tttcttcatc ctttttttca agttcagcta tttgtctttt aatttcttca tctataactt
     2161 ctttatattt agtattatcc ttaaacaagt ccattaattc taaaaatttt ccagttctca
     2221 tatccacatt atatgctata tcttgataaa atccatgtgc tccaccacta taagtataat
     2281 atctcacttt tatactaaga gcagaatcag tattcttttt aagttcaaag tctacatttg
     2341 caacaaactt atttttctcg tcaggattat ccttaagata ctgctttgct tctttatatg
     2401 atttattata aaattcaaat atgtcatttt ttatcttatc atttgtagac ctttcaacaa
     2461 ttttatttga atttgttatt acaggcatgt ttatagtact ttctaaatac tcatcatttt
     2521 tcactattgt tttacttcct attgtactta ctgataaagt ttgtttacta tttgtattac
     2581 cctttggtaa tgactggtta ctcataacag tcactgcttc cttattacta tttaaattaa
     2641 ttgaagcatc tttccttaaa ttattatatt tatagtcatg tttaattgcc aaaacagcca
     2701 ttatagtcaa tgttaaaaca aatacatatc ttaaaacttt agcttttgta tcgactttaa
     2761 aaaatttcat attatcattt tcctcctaaa tataaatctc tcttattata tttggagttt
     2821 ctagggtttt ttatccatat aatttaataa aagattaatt tttatttata aaaaattaat
     2881 ctttttgttt taaaatatta atatgtctta tcaaacagtt tgaaatttca ccaagaggta
     2941 attgtaacat aacagaacct ctatcaaaag ctaccattgg cattccatat actatacaag
     3001 aattttcatc ctgtccaatt gtaaatgaac cattttcttt catttttaaa agtccacttg
     3061 ctccatcata acccatacct gtaagtatta ttcctattga atttttacct gctacttctg
     3121 ctactgaatc aaacaaaacg tctactgatg gacagtgacc acttattctt tctccttttt
     3181 cacaagttac ataatatcct tgtttatccg tacacaattt catctggtaa cccccaggtg
     3241 caattaacac atttccttgt tctactctat cattattctt agcttctctt acattcataa
     3301 tacactgctt gtcaagccgt tgtgcataca tataagtaaa tatagctggc atatgttgaa
     3361 ctacaacaat ccctggtgtt gattttggta ggtcttttat aatttcaagt attgcctctg
     3421 taccacctac agatgcgcct atagcaataa ccatattatt aaaacgttcc acattaccgc
     3481 ctccaaaaaa tcatatatca aaaatacctt tttatcgttt atatatagca ggttttacat
     3541 atttgtattt agtactactt ctgttgatta cttctgcatg tcctacaaac aaaaaactat
     3601 cttttttggt agcattataa aatttattta taagttcttc tcttgttttt aaatcaaaat
     3661 aaatcataac atttctacaa aaaatcaaat caaaatgttg atatgagaaa tcattcatta
     3721 aattaaacgg cttaaaaatt acactttttc taatcttatc acaaatttta aatgtaccat
     3781 ccttgttgtc tataaaatac ttcttcttcc ataactcagg aacattgtta acatttgatt
     3841 ctatgtatat acctctttta gctttttcaa gtgagttagt tgaaatatct gtggctatga
     3901 ttttaatgtt ccacaactct ttattatatc caaaataatc atccaaagtc atagctattg
     3961 aatacgcttc ttctccacta gaacatcctg cactccatat atttatgttt cttattttat
     4021 tattttcttc ccaaaaagga agtatactat tctgtataaa ctcaaaatgt tgaggttctc
     4081 tcataaaaaa tgtgtggtta gtactcaatc tatttattag gtttatagct tcatttcctg
     4141 tcctatcttt aaataataga ttcatatatt gactaaaaga actaagtttt ttctctatca
     4201 ttgtattgta taagcgacct tcaattaacg ctcttttttt acttaaatca ataccatatt
     4261 ctttttttac atggtttaca agaactataa attcttcatc tgtgagtttt atcatgcaaa
     4321 aacttcctcc tgctaattta tcagaactaa ctatatgcta ttaatcaaaa actttcgaat
     4381 atctaaaatt aatatagaat tatcttcaac tttcgcaatc tcttttatat ctttattaat
     4441 attagtgttg ttagaattag tcgtctctag taaatttaga ttttctatat gaataacatc
     4501 aacaacttta tctacaataa ttccaacttg atactcatca acctttgtaa ttattataca
     4561 agttctctca ttataatcag ctttaggaag attgtacttt atatgcatat taataactgg
     4621 tattattctt cctctcaaat taataactcc acttatatat gttggaagac atggtacata
     4681 cgttattgat tgaaatctta tggtttcaat cacattttct aattctattc cataaataac
     4741 atcattgaca taaaatgtta atacttgttt aataatttca tctttagcca tactttaaat
     4801 cccctaatat atttctataa tgctatctgc atctataatt aaacttatac ttccatcccc
     4861 aagtattgta catcccgata aacccttatt ttttatatta aatcggttaa aataaactgg
     4921 aaatggtttc acaacaactt gctgattacc tattatttca tcaacaaaaa tgcatactgc
     4981 tttatgctca ttttcaataa ttatcatatt accattcatc agattggtta cttttgtttg
     5041 aatattatat attttatgta accttataat gggaatacat gctccatata ttgtaattaa
     5101 ctctgtctta tttccttctg taattatttg atttaagtct gtaagtttaa atgttcttct
     5161 tatggatgat attggtaaag taaatagtga atctccaact ttaaacttca taccctctac
     5221 tatagaaaga gttaatggta tttttatagt aaaggttgtc ccctcatcct gtttactttt
     5281 tacttcaatt gaacctccaa ctttttcaat acttttacga acaatgtcca tgcctactcc
     5341 tcttccagaa aattcagtta tagattcttt tgtagaaaag cctggagtca taatcaaaga
     5401 attgacctcg tcatcagtat actcattact ttgatgttta agaagacctt gttccttagc
     5461 ttttgccaaa atcttatctc tcataatgcc tctaccatca tctgaaatac ttatcacagc
     5521 ttctccgcct agattttcag cagataaaac tatctttccc acttcttctt tacctttcgc
     5581 tatacgctcc tcaataggct ctattgcatg gtccatagca tttcttataa ggtgcataaa
     5641 aggttccgct aaattatcaa ttacactctt atccacctct gtttctgctc ctatagttat
     5701 taactctaca tttttatcta attttttatt catatctcgt acaattctat gcattttttg
     5761 aaatacacca gcaatttgaa tcatacgcat agacattact atatcttgca attcatctgt
     5821 aagcttgtgt aattgtcttg ttgccttatt gaaattatct atatcaacac ctgtagaatt
     5881 tacgctattt acaaccattg attccataat tacaatttct ccaactatat tttgaagtaa
     5941 gtccaatttt tctaaattta cactcataat actctgctta cttattggta aatgttcatt
     6001 ttcttcactt agactatctt tttccatatc agttttttca tttaaaacat cttcttgtat
     6061 ctcatcttct gtatagttat tttgataact tttacttact ttattattat cttttaattt
     6121 attttctact gcatcattgt ttgatgtact tttcatttct atttcttttt tactaatatt
     6181 tatgtattct tttacataga gattatcttc tatagtttta taaactattg ccttatcctc
     6241 tttagctaca aattgtatat aaaacccata acttttaata tactctgtag actctgcatt
     6301 tgtttctaca tcttttggat aaaaatctat aaattcacag taatctctaa gttcaaatac
     6361 aagcatgaaa gctctcacat tttccatcat gcaaccttgt tcaaagatta tttttactac
     6421 atcagagtct tctttgtatt caaaatttga atcgattact tctttttcgt ctttactttc
     6481 taaatttgca ttatcctttg atttcataga acttgataat tccaaagtac tcacatactc
     6541 ttcaattcct tcaataaaat tagagtaatc agaagatata caacctcttg attgtaactc
     6601 atctatatct cttcttataa aatctaatga ttgaaagact aaatcaaata ataggtcatt
     6661 gtctaaaact tctattttac cttcacgtat agcataaaac aagtcttcta cactatgtgc
     6721 taaagcagat atattgtcta accccatcat agatgctgaa ccttttatcg tatgcattat
     6781 tctaaaagtt tcattaatgt tgttatgagt aaatactttc tctttttcta tttgaatcat
     6841 aatttcatct aattgttcga gtaataaact tgtctcaaat ataaacatat ctaacatttg
     6901 tctcatactc ggttcaatat aattcacatt tatcacctac gacctacaaa taattttcta
     6961 ttatatatgc atctcataat ttaaatcctt gatgattctc ttatctcact atatgtacct
     7021 cataatttaa atacacaatt attctcttac ttcaatatat atacatctca taatttaaat
     7081 ccttgataat tctcttattt caatatatat acatctcata atttaaatac atgataattc
     7141 tcttacttca atattcacct cataatttaa acccatgatg attctcttac ttcactttcc
     7201 tttagatgaa atttttctac taattctttt aaaatttgag cctgagcaga catctcttct
     7261 gaggctgctg cactttcctc tgctgttgct gagttcattt ggactactat ggaaatttgc
     7321 tctattccag ataaaatctg tgtaattgct acttcttgac tatgtacagc cgaagaaata
     7381 ccattaatat aattagtagt tttttctaca ccatttacta ccaagtccaa tgcatttgca
     7441 gtctctttag caattctgac accattttct actgttttaa tagcttcttc tatgagaata
     7501 gctgtatttt gagctgcttt tgcactttta ccagccaaag acctaacttc atctgctaca
     7561 actgcaaatc ctctaccata ttgacctgct cttgctgctt caacagctgc atttaatgca
     7621 agaatattag tttggaatgc tatctcatct attgttttga ttatttttga tatttcactc
     7681 gatttttcac ttatttcacc cattgatgtc atcatatcat ccatacatct acttccaaaa
     7741 ccaacttgtg atgcagattg gtcagatacc tcacatgcaa gactagcact atctgcacac
     7801 ttcttaattc catctgatat ctcattaatc gtggaagcta attcttctac tgaactagct
     7861 tgttcagtag caccctgtga aagagcttgt gcacctgctg caacttgttc tgaagcactt
     7921 gcaacttgct ctgctgataa atttatttca gaaaaatcac cattaaaaga gtttattata
     7981 ctttctaaag atattttaat tgatttaaaa tcacctataa attcttggtc tatctctact
     8041 cttaaatctc cttttgcaat actagttaat gtactgtcta taaggtctat gtaatttaaa
     8101 agcatacttt gcatcttttt gacagaactc actaatagcc ctatttcaga tttactttct
     8161 tcaacgtcta tacttgttct taagtcccca gatgcaattc ttgaaaagtg tttttctact
     8221 ttattaatag gaatcactat ttttaatttt attactgtat attctattat tgtcaatatt
     8281 attaaaaggg aaagtataaa tattgttctt aatattagac tttgtaattc tttttttact
     8341 tctacaattt tggaatttga acgggtaact atatcctctt gaaattcatt tattaaagaa
     8401 tccattttct cttgggagtt tatatagtca ctaccaaaaa ttatactttg agctttactc
     8461 ttatcattac tttcgactgc ctttattgca ctttgttctg ttggtactaa agaatctgat
     8521 atttcttttg cttctaagat tttatttgac tcgctctttg taagaccaat tttttctaat
     8581 ttgtttaaag ctgattctct tgtttttccg tataattctt tttcataaat atctttattc
     8641 tttttttctc ctgtaaatac atatcttctt aatacatcac ttatctctct agttgtatct
     8701 tttatatact gactttgagt cctcaaatta ttcatatcat taatactttt ttctactttt
     8761 tcaaatgctt tataagatga aatagaagaa aaaatccctg ctagaacagc caatactata
     8821 ataatcagca atacaattgc taatttagaa agctttactt ctcttttcat atctgtactc
     8881 cttcataaag taagtttata tcttttttaa tcctttcatt gtactcacta cgctacaatc
     8941 ttttcctaag tttttaccct catacattgc tttatctgct aaggatatgt tttcttctaa
     9001 tgatatattt gagtttactt ctactactcc taatgtaata gtgcacttta ttttatgttc
     9061 tttatagttg aaatcaaact cctttatctc tttacggatt ctttctgaaa gtatcttagc
     9121 accttctatt ggagtatttg tgagcattat caaaaactct tctccacccc atcttgatac
     9181 agtatcaatt tgtctacaat tcttttttat tatacttgat attactgtta acacgaaatc
     9241 ccctgcttca tgcccataag tatcattgat aaccttaaag tcatcaatat caccaaggat
     9301 aagggatatt gatgtttcac tatatttatt aattcttctt tgctccataa gctgattaat
     9361 tatatgtcgc ctattgtaaa gacctgtaag ataatctctt acaacgagtc tattcaattt
     9421 ttcaagagaa gattccagtt cattatttat cttttttaat ttatcttgag atttctttac
     9481 ttcaagatga gctataacac gagcttttaa ttctgtcata ctaaaaggtt tcaccatata
     9541 gtcaattgct cctacaccaa atccttttac tatgtctgta tctgtatttt ttgaagttat
     9601 aaaaattata ggtatatctg ctgtacgagc attagatttt acttccttac aaaactcaaa
     9661 accagagtta tctggtaaaa ttatatcaag caaaataatg tctggtataa attcttcaat
     9721 aaattctttt gcctctattg cattaaatgc ttttttaacc ttaaaattat cttttgtaag
     9781 tgttttatct attaagtcac acatcagaac actatcatca acgactaaaa ttgttccctg
     9841 ttgcttattt ttcatataca aacctctctt aaacataata tattcaaatt ttaatgattt
     9901 aaaattttta tacttaataa ttataccact atattcttaa atgttctcat cacaatcttt
     9961 ttataataac actccaacta catccctatg ttacatgata gtgcaatcaa acccattgat
    10021 taattcatct attaaaactt catctttatc tgtcaaaagt ttctccaaat ctagtaaagt
    10081 aattatttta ccttctaatt ctccaactcc acttacaaaa taatccgaat attctatagt
    10141 aactcttggt ggaggtgata ttttactttt ttctaagctt actatatcat caactctatc
    10201 aaccacaaat ccaactacca agtcctttat ttcagttact actatataca tgcaatttgt
    10261 agtcattaat tcatcttttt taagtctcaa acgcatatct attatcggaa tgatttttcc
    10321 tcttagattt attatgcctt tagaatatgt aggtagttct ggaataaata taatttcttg
    10381 atttccagta atttctatta ccttttcaag ttcaacccca aaatcttgct catctacttt
    10441 aaactttaga tacttaattc cttcatcaat tagtccataa tctaaatatg ggtcatctgg
    10501 aaatctcatt taatacctcc ctcctaaaat acactttcta tgaaatatat attataattt
    10561 cttcttacct ttatttattg aatctacaag taattcaccc gtctcagtgt aaaaacgtat
    10621 tgttctacca taattcatac ctgtgtcctc agctaatatt ggaatattta atcctaaaag
    10681 agtttttttt actgcctcaa cattcctatg tcctatactt gcaattactg aattgctact
    10741 tcctaaaaac atctgagcac ctccagcaat ttttgctttt atatatattc tatttgcacc
    10801 tatattctcc atagatttaa tgagctcatt tattcctgtg tcagcaaatt tacacttatt
    10861 ttcaatgtta atagcatctt tactatatgg caacataacg tgtaacattc caccaattcc
    10921 agctacttta tctatcaaac atactcctac acaagaacca agtgcataag taactaaaac
    10981 atttggtcta taggctatct tcatatctgc aatgttcact actatctctc tattcatact
    11041 tctaatccca acctcacgag tatttctcta agtgagctca tttctggaat catcaaaatg
    11101 tgactagtaa aataattatc actcatttta aatttatttt ctataaataa gactttgtca
    11161 ccaacatctg aaaatcttat cataggtaca tttagaacag ctccaaccat atcaatacat
    11221 atatctggaa ttgaaacact tatagtcatg tttaacatag atgcaatagc atttacatat
    11281 gaacttgcca taatatttcc tatttcctta attgctgaaa tttcaattgc ctcaatctca
    11341 tctaaaaaag cttcttcttt attaaaaaga gaacttagca taagttgagt tattgattca
    11401 tccaacaaaa acataaacat tccattgata tcacctttca tatcgagtaa aatacatact
    11461 actttgtttt ctggaccacc aagcaaattt gtcgcttcat tatattcaag tatcttgacc
    11521 ataggtatta atacttctac attgctattt atcatggatg caagtgcagt aattgcattc
    11581 ccagagccta tattaccaat ttctctaaga gcatctatat gaacatcatc aagttctaaa
    11641 taatttataa tataaacccc tccctttatt attgcattta tttgcttttc attgtattag
    11701 tgtatattta cacttttatt ttaaagcatt atctacaact ttaactaatg tttctgtttt
    11761 gaagggcttc actataaaat ctaacgctcc aagttttatg gcttctacaa tcatattctc
    11821 ttgtcctatt gcagagcaca ttacaacctt agcacttgaa tccaactttt ttatttctct
    11881 tagcacctct aaaccatctt tttttggcat tgtaatatca agcaacatca aatcaggctt
    11941 ttcatattta aatatattac acgcttcttc tccatcattt gcttctatta aattattata
    12001 gccactttta ctcagagctt ctttaattat cattctcata aaaatagcat catctactat
    12061 caaaatttta ttttccataa aactaactcc tccatatgta caacaataaa cgcttttatc
    12121 ttcagttatt gtctatacta tctgactatt tttcgctaca caataaatac atttcattga
    12181 taaataaaag taattattta tgccatatta tacttaattc tcttaacata atttgtcaac
    12241 aaaaaaactt atatatctat tgtaatatat gtttggcgtt ttgacgagtt attaaaaata
    12301 taattgtata aaacataaaa agttatcaaa agtaattaaa ttataataaa acaactttat
    12361 acctttttta tgtttgtata aaaaaaattt tcagattcat taaaatcatt tctgtagatt
    12421 catataggat ttgactttct caatatctaa tagatagttg tttatttact taattatatt
    12481 tctctatatg tattatcgat taaattagat ttagctttat acattatttt ttatattttt
    12541 caaattgtat actcataaat ccagaagtat tctaatgtgt ttataatact ttttttcata
    12601 ttttaaatta aataacaaat cattatattt acaagtatca tttatcaata aacaatatta
    12661 aatgaagaat atgtctgaag attcaaagtg atactgatac tattaattac taaaaaagca
    12721 gtaaatcttt tacagattta ctgcttatag ttataaataa taaaactatc tataataaat
    12781 gtactatact caatatttca ttcaatatag taataacaaa tctactttat tcctatgtaa
    12841 atatgaattt cttgtttctt tgtatcaaaa gtattacatt gatactcttc aaaatcactt
    12901 atataagtcc tatcaaaatc catttgccaa aactcaaacc aaaaatctcc tacttctttt
    12961 tgacctccta ctataacaaa ctttgcatac ttacctgctg gtataacttt actaataata
    13021 ctttctccaa cagtattatt agtgtttaat tcttctaaat tcttctcttt atcagaatta
    13081 ctatttactt cacaacatgc aacaaagcta tacggacttg taaaatcacc ctggtaatct
    13141 gtatatagac ctattgtctt gtcattcttt ttacccttta tagcattata tatccctttg
    13201 cccataaatt ttttccacaa ttcacctata tcatttaccg cttgtccatc tttatttgta
    13261 gtctccttcg atattcctat aactattttc tcatttatct ctactatttc ataattcata
    13321 ctttatacct cactttgttt ttattaatct attttatttt gaactaattc aattataatt
    13381 tataatatac gacaccttta tgtcatattt ttaatataat taaactcagt gagatattat
    13441 atactaaatg caagatatta tatatttaat aaaatcttta atctagcatt atattataat
    13501 gaataaattt tagacaactc ccttagtcta gttattacat tatccctaac atctttaggt
    13561 tcaagtactt caacattgtc accaaatgat aaaatatatc cataaatcca ttcaccaata
    13621 ggaaattctg taattacatc atatgtatca tcttcatttt tagttattaa atccttatta
    13681 aaatcatcaa atactctaaa aagcatcttt tctttaaacc tcaatttcaa cgttatagta
    13741 ttttccttaa ttactttaga atcattcaaa catacttctt ctattatctt tcttgaaaat
    13801 acttcatcct ttatagataa attccttatc ctactaattc taaatatcct aaaatcatct
    13861 tttagtttac aaaatccata caagtaccaa gtcttactct tatacataag cttcagtggt
    13921 tcgacagttc tgtttgtctg gcttgacaaa ctatttacat aattaaaatt tatagtatta
    13981 cgccttaaaa tagcttcttt tatttttgta aatttatcat cttcattaaa attacttccc
    14041 cattcagtaa agtctatttc aatccagttt gaaaaattct gctctccaaa tattgagcca
    14101 attttattta ctactgaatt tatttctgga tacttagtcg cctgtaatgt tttcagtgca
    14161 actattaaac tctctttatc tttttttgac aatatagcct tgtctataga atattcttct
    14221 attaaagata tccctcctcc tttgcctttc gtcatatata ctggtactcc cgacatagac
    14281 agagtttcta tatctctata tatggttctt gtagaaactt caaatctatc tgccaactct
    14341 ttagctgtta ctaatttttt atttaataga attactatta tttctgttaa tctatttatt
    14401 ttcattcatt accacctact aaaattatta aatttattta atctaaatta atcatactaa
    14461 tttttaaata aataaaatct tagaatctta tctaaattat ttttacatga ttttatttac
    14521 tttctattat ctttctcact ttggatatta tcatcttaat ttatttatta ctatatcaat
    14581 ttacttatta tcatctcatt tgttatcgtc ttacttaatt tacttatttc tatatcaatt
    14641 cacttattat tacctcactt gttatcatct tacttaattt acttatttct atatcaattt
    14701 acttattatc atctcatttg ttatcgtctt acttaattta cttattgcta tgtcaattca
    14761 tttattatta tctcacttgt tatcgtctta cttaatttac ttatttctat atcaattcac
    14821 ttattatcat cttaattcat ttgcttatta tcatttactt tattcaattt ctatatcaaa
    14881 attagtattt gtacttaata cttaaaatta atttaaagtc tttacatatt atttattttc
    14941 tattgtaata ttcatttcca tactttacta agctacttcc actcatttat tttaaaattt
    15001 caatttacta tttctcaact ctatattatt atttttcaat tttatattac taattttaaa
    15061 gctttttagc tattattcct acaatattta aacaattttt tcataataaa gctatattac
    15121 aatatattca aaatatgtaa tattttagat atttttcgtt gacaatgtta aatttttgtt
    15181 ttatccttaa ttaaggacat gttattgaca tttctgaaac ttatttgaaa tagttttttc
    15241 attttattta ggaaaacgta ttatacttaa gaaaacgtat tttctcattt cagataagta
    15301 aaatatttat tcttttgggg aggaaatcat ggaaattaat aaaaaacatt ttattcaagt
    15361 atttttaact tgcttaatct tggtagtaat atgtgaattt atagggcaaa gaactatagc
    15421 tattggaaaa gcctctatag tactgttccc tatgctttat gcagttataa ttggacttat
    15481 aataacacca gatttattag gtaaaaaaat taaagcactt aaaaaagtgg ttggagaaaa
    15541 agaaataaat attgctggtg aagttgttgg aattgcacta gtaatgcttg gtataaaata
    15601 tggtactaca gttggaccaa acattgacaa aataattcaa gctggaccag cgtttatcgc
    15661 tcaagaattt ggtcatatac ttgcaccaat cgttgcatta cctcttgcat taatgtttgg
    15721 aatgaaaaga gaagctgttg gagctactgc aagtataagt cgtgaacctt ctttaggagt
    15781 tatatcagaa aaatatggga taaactcacc agaaggtagt ggtgtacttg gtacatattt
    15841 aatgggaaca gttcttggta ctatatactt tagtgtacta ggttcaatat ctatttatag
    15901 tggtcttcac ccatatgctt tgggaatggc ctgtggtgtt ggtagtggaa gtatgatgac
    15961 agcagctgct ggttcattat ctaccatggt accacctgaa atggcagata caatacttgc
    16021 ctatgctgca acaagcaata tgatgtctag tataacagga attatattct tagtttttgt
    16081 atccttacca tttactaatt ttatgtataa gattttagaa cctaaatttt cgagaaataa
    16141 aaaaactaaa gaaattaaat ctacagaagg agagtgctaa taaatgtttg ctaatttaaa
    16201 tttaaagaaa agttcatatc aattattggt tatgtctgtt attggagtta taattcttat
    16261 aggacaaaga attagcgttg gtacatctat agttacagct cttcctggta tggtgatgct
    16321 aatattagct gctatggcag ctatgattat aaaagattta tttcctaaat caatctttcc
    16381 agcttttgga tttgcaacta taatcggcct tttacttagt atgccatata gtccaacatc
    16441 tgaagtattc ttaactaaca ctaataatat taactttatg gctataacta ctccactact
    16501 agcttttgca ggaatttctg ttggaaacaa aatagaagct ttaaaagaaa tgtcatggaa
    16561 aatagttata atttcactta ttgtatttac aactatattc tttgcatgtg cgagtatagc
    16621 tcatatagtt ttaagtattc aaggtaaaat ataggaggaa tttattatga ataaagaaca
    16681 attaaaatct ctagtaataa aaactataga tgaaaataga gaaaaaatat taaaaatagg
    16741 tcaaggaata tacaaaaatc cagaatatgg atataaagaa tttaaaacta cagaagctgt
    16801 ttcaaacttt tttgagcaag aattaaattt atctgtagaa aaaaatatag cagttacagg
    16861 atgcaaagca gatgcaaatt ctagcaagaa aggtcctcat atatctatac ttggagaatt
    16921 ggatggtata tcatgtaaag aacataaaga tgctaatgaa ataggtgctt ctcatacttg
    16981 tggacataat attcaaatag ctggaatgtt aggtgctgct gttggtttag taaaaagtgg
    17041 cgttttagaa catttagatg gtaaagtatc ttttatggct actcctgctg aagagtttat
    17101 tgagttggaa tacagagagc aattaaaaaa agaaggaaaa ataacttatt ttggtggaaa
    17161 acaagaatta ataaaaagag gttattttga tgatatagac atatctatga tgtttcatgc
    17221 ttctgattta ggagaagata aggctctagt tggtcctgag agtaatggat ttgttggaaa
    17281 aaaggttaaa tttataggta aagaatctca tgctggttct gctccacatg atggaataaa
    17341 tgctttaaat gcagcaatgc ttgctataaa caacgtgaat gcacttagag aaacttttaa
    17401 agagtctgaa agagttagat tccaccctat catcacaaaa ggtggagata tagtaaatgt
    17461 tgttcctgct gatgttcata tggaatcata tgttagagct agaactataa atggtatgat
    17521 tgatgctaat gaaagaataa ataaggcttt gatggctggt ggaatggcag ttggagctga
    17581 tgttgagata actgaaattc ctggatatct tccaatatta cgatacaaaa gtcttgataa
    17641 tttatttaaa aacaatcttg aagatttagg tctaaaagga aaagttattg atggtggaga
    17701 ttttacaggc tcttttgatt ttggagatgt gtctcatata atgcctactc ttcaccctat
    17761 gattggtggt gtaaaaggag ctcttcatac aagagatttt gaaatagttg atgaagattt
    17821 agcttacata attcctgcta aagcaatggc acttacagtt gtagaccttc tttttgataa
    17881 tgctaaggat gctaaagata tattagatac ctttactcca gttatgacta aagaagaata
    17941 tctaacattc ttagaaaaac acgataaaac tttataagtc tatttttata atagggtggt
    18001 atctactgta aaagttatac cactctttgt ttttgtaaca gtatacttct tttcttttag
    18061 tttttagatt aaactaaaat atattatcct ccttaaaact tttagagcta cgttgaaata
    18121 tcagtagaac tatgctatat tctttctaat taatataaat ttctattcta attcaaggtg
    18181 gtgaaaaaat gtttaatgca ggaaatcgaa ttataaatac atggttgtac cctattcaag
    18241 atggctttgt cttgattgat actggatatg aaaatggttt taaacatttc aaaaaacgaa
    18301 tttctaaatt caacataaaa atcaaagata ttcgatacat atttctaact catgctcatg
    18361 atgaccatgt tggttttcta aatcagattc tagacgaatc tcctaataca aagattgtta
    18421 tgcatgataa agctttagaa attctatata aaggtcaaaa ctcttttatt ggtggttgta
    18481 ccagccgtac tgcctttctc ttttgccaaa ttatgaaact atttggaaaa ggtgagcata
    18541 gtttttctcc acttaaacaa gaatttgaaa aaaggtgtat tcttgtgtct gacaagaatc
    18601 gtaaagaaat tgaaaaagaa ctaaatggaa aaattataga tacaccagga catactgatg
    18661 attcaatttc actactaatt gatgaaggtg ttttattttg tggagattct gcaatgaatg
    18721 gatttccaag tactcacaaa attaccatat ttgcagaaag caaactagaa tttattcaat
    18781 cttggaaaac aatcatcaat acaaaaccta aaatgattta tcctggacat ggaaaaccat
    18841 ttgaatacag taaattagaa caaaatctaa tctatgtaaa taaaataaat ttatatcctc
    18901 tacataattc aaaagatatt cacagatttt tgtagaaaat acaaagtaaa tttatttgat
    18961 taattaaaaa atctaacact ttactacgct acttatttta ataacttaat cttccactag
    19021 cttttcataa aattttgttt taaaattaat tttaaagtgc atatgttatt taaatacatt
    19081 agataaaatt cgtcattaat ctacttaaag catatttaac taacaccact caaccagaaa
    19141 attcagaaaa ctgcattgac acaggaaaaa gtttatttta tacttttttt aggacatatt
    19201 gtggacacgt cttaaatcga caaacagaat ttaaattgta agattaacca caatacttat
    19261 tttttaatta tatttttaaa cattagggag gatattatga atgataacgt agttaagaag
    19321 aaaaactttt ttgacaggac attaaacaga atagaaactg ttggtaataa actaccagac
    19381 ccagttacaa tatttttagg gttatgtgta cttttactta tactatctag tctagttggt
    19441 tctatgggta tatcagtagt tcatcctggt actggagaaa ctataaccgc tgtaaattta
    19501 ttgactgtag aacagcttca aattctttta ggaaacatag tcagcaattt ccaagggttt
    19561 gctcctttgg gtctagttct agtaactatg ataggtgctg gtgtatgtga caaaacagga
    19621 cttatgacag caacaatcaa agcatcagta agcaagattc ctgaaacaag agtaactctt
    19681 gttgtaatga ctataggtat gcttgccaat atagcttcag atgcaggtac aatattattt
    19741 ccaccattag ctgcacttgt atatcttggt gttggtagac atccacttat aggtttattt
    19801 tctggatatg cagcagtctg tctaggattt gccgcaaaca ttatgataag tgtcaatgat
    19861 atacttgctg caagttttac agttccagca gctcaaatga ttgattctgg atttcaagcc
    19921 aatgcaacaa tgaacttatt ttttatgata gcatcaactt ttgttctaat acttttggca
    19981 acatgggtaa cagaaaaaat tgtagctcct agatttggta aatatgaagg aaatgcagaa
    20041 ctagatgttg atggaagtct tagtgatgta gaaaagaaag ggcttaaaaa agctagtata
    20101 agtatattaa tatatgtggc tattatagta gctcttagtg taatcggtaa aaaaccattc
    20161 ttagctgacc cagaaacagg agatttacta tcaaacaatg ctcctcttat gaaaggtatg
    20221 gtacctataa tagcactagc atttttcata cctggtgttg tatacggaaa aactataggt
    20281 aaaataaaga aggataagga tgtagtctct ataatggcta gtgcaatgag tgatatgggt
    20341 ggatatataa tactagcatt tgctgcttca caatttttgc aattattcac aaatagtaat
    20401 ttaggtctga tactagctgt aaagggcgga gaatttttaa aatcagcagg aataaatgga
    20461 cctttattgt tgataggatt tattatatta tcatgtttta taaatttatt tataggtagt
    20521 gcatctgcaa agtgggctat tcttgcacct atatttgtac caatgtttat gatggttgga
    20581 tacaatccag cacttactca gatggcttat cgtataggtg attgtataac aaatccactt
    20641 agcccattat ttccgtactt ccctattata ctagcattca caagaaaata cgataaagat
    20701 gctggtatgg gtactgtaat tgctaatatg ataccatact ctatatcatt cttaatagtg
    20761 tggactatac tattaatatt gtttatggta tttaatattc cactaggacc tggaatactt
    20821 ccttcatata gcatgtaatt tatttaaatc tttctaaaag gagaaataat ttatgttaga
    20881 taaaaataga cttataaata attttatgga tatggttaga attgatagcc catctaatca
    20941 agaattagaa atgtctaagt ggttggtaaa ctacttaaaa gaaagaaata tagatgccat
    21001 tatagatgat gcaggtgaaa aatatggtgg taatacaggt aatgtcattg cctatataaa
    21061 aggtgaagaa ggaagtagac cactttgttt atgtgcacac atggaccaag ttcaaccttg
    21121 tttaggtgta aaaccaatac tagatggtaa tgtagttaga agtgatggaa ctactacact
    21181 tggtgcagat gataaagctg gaatagctgc tatacttgaa gctttagaac atgttattac
    21241 agaaaaaata ccacatagag atatttatct ttgttttaca atctgtgagg aagctggtat
    21301 gcatggtgtc aaaaacttta atcctgacaa cttgccttgt aaagatatgg tgattttaga
    21361 ttctggtgga gctattggtt cgatagcata caaagctcct gctcaacaat ctataaaaat
    21421 ctcttttcaa ggaaagaaag cacatgctgg tatagaacct gaaaaagggt taaatgctat
    21481 attagtagcc tctcatgcaa taagcaacat gcacataggc agaatagatt cattgactac
    21541 ttcaaatata ggtgaaatag aaggtggtgg agctacaaac atagtaactg ataaggttac
    21601 attgactgct gaaattaggt ctcatatacc tgaaacttta gagtatgagc ttaatcatat
    21661 ggaaaaatgt tgtaaggacg ctgcatctaa atttaatact acttatactt ttgaacataa
    21721 tatgtcttac cctagttttg aacttagtag agatagtcat gttttcaagt taagtgaaga
    21781 agcaataaga caagttggtg ttgttccaaa tccaatggta attggtggtg gaagtgacgc
    21841 aaatatatta gctaatcttg gatataattg tgctatatta agtctaggta tgtatgatgt
    21901 acacacagtt aatgaatatg taaatataga cgaattatat gatactgcca aaatagttta
    21961 tcatatgatt aaattataaa tatcataata gaactcttta aattataaaa ataaaaacct
    22021 atgtatcctc aactttataa gttatatatt agagttgttt tacataggtt ttagtatttt
    22081 tatataataa tttgttttaa catttttcat aataagcttt gatattttat atagtaattt
    22141 gttctaacat ttttcataat aagcttcaat attttgcata ataatttatt ctagcatttt
    22201 taataatagg ctctagtatt ttatattaat aatttattct agtattttac ataattattt
    22261 gttctaattt ttttcataat aagctttaat actttatata gtaatttgtt gtagaatttt
    22321 atacaatata atgatttata cagttttata aaatatctta aaacagtatt tcagtaatat
    22381 tttttggtct tcctccacca atactagtct cttttgcaca tattcgacct aaacctgcat
    22441 ttactatttt atttaatata cgtcttgcac ttctttcact tacatttaga tactctgcta
    22501 actcttttga atcataaatc ttactttttc tattctcatt tatagcaata atttttgcaa
    22561 ctgactcaca actcattcca gtctttttag atatttcgat taccttttca tcagatagca
    22621 tcaaagaata atttaattca ttttcggaag ctaaaggtcc tcttatgttt tcatcttcat
    22681 ctaccaaata tataccaatt tcttttgaat ctatggagtg tcttaatgct ttatgtgcat
    22741 tattttcagc ctgatacgca gttactccag taccaattcc tatactaaga gaaaagccca
    22801 tgtcttttat ctctctcttt agattgaata gcttatcata attatactca ttatcagcta
    22861 cccctttatg aacaaataca acataatcat ttcttccaag taaaaataag gaaccttgaa
    22921 tgcttcttac atagtttact acaagcttat ccatatctga tttttttatc atgtcagaat
    22981 aatagttatc tatcttctct ttataatcta ttaaactcaa tatttcaaca gcaatttggg
    23041 aaaatcttgc cttacttaga gcatattcac tctttacctt gtcataactt tctttgatta
    23101 attgtatagt agcttgtaaa cgaaatacag gatagcctct ttcttttaat tcattatata
    23161 ctgcaccaaa accagtcaac attatatccg tcttattttc ttcaaataat tttatatgcc
    23221 attctacaag ttcagactct cctacttcca aagaaaatgg attacaaaat acactcgttg
    23281 ctttaatatc aaattcttta agtgcatctt ccacaacata tctatcaact acatctatac
    23341 tgaatttatc taattttata tttgcacttt ttattgccca aaatgccttc attatactcg
    23401 tcccacttct cggtacaaat gaatgtggct tacttatctc atacttagat tttataaatt
    23461 cataaactgc acatcctgta aatataatag catcacattc gtcttcacat ttagaaataa
    23521 cttctattgt atctactact ttttctctta catatatttt agtctttaaa tcactgtcta
    23581 tctgttttaa atcatccttt atcttaaacc cagagtctga aggacctata atacccacta
    23641 tcataaactt cgctcctaat ccataaatat tatacttttt aaaaattata tcctcactat
    23701 tttctacttc tatttaatta ttttgaacta ctccatccta cctacattta gaatggaata
    23761 attagcttat ttttgataaa gtaacttctc aaaatcagca cttggaatag gcttacaata
    23821 ataataacct tgaattacca cacaatctaa agtcactaaa aattcagctt gttcttttgt
    23881 ctctacacct tctgccacta catccatatc taaatctttt gccatggcta tagtgtgctt
    23941 taaaactatt ttttctctgt ctgtgattcc ttcctcatta aagaaaccac aatctatctt
    24001 taatgtatca atattaatat tttttaacat atttaatgaa gaatatgctg aaccaaagtc
    24061 atccatattt aaaacaatac catccattct aaggtcggct attatcttag taaataattt
    24121 tatattttta ataaataaac tttctgtaag ctctaattct aaaaaagatt ttggtatact
    24181 atatttattt attaaactta aaacaatttc tttaaaatgt ggattatgca aatgtaatct
    24241 tgatacattt acagagatag gaactggctc ttcacctaaa tctatccatt ttctaatcaa
    24301 agaacatact tgttcccata catacatatc taatctgata ataaacccat tcttctcaaa
    24361 tagtgaaata aatttaattg gaggtattat acctttttct ggatgaaccc atctaaccaa
    24421 tgcttctgca ccaacaattt tatttgcatt aagattatac ttaggttgta aatatacatg
    24481 gaattgccca ttttcaagtg cgtattccat ttccctttcc atctgttttt cttcaagtgc
    24541 atcatttctt atagtatcat cataaaatat atagttattg atactttgtt tctttacttt
    24601 tttgtgagca tatcctgcca tttcacacgc atttgtaatt ggaatatgat tatccaaaat
    24661 tctataaacc ccaaaacatg gtactaacat atattcaaat ttttttctat ttaagctatt
    24721 tttaatctta taaattacct tatgaatatc tctatcagag taactctcat aaaaaatgca
    24781 aaatacatct gcatacatac gacaacatat accattagaa ccaatgtttt tttgaatcaa
    24841 ctctgcaaca tatattaaag ctttatctcc tcctctaact ccataaatgt cattaattac
    24901 cttgaactta tctatatcca tcattataat tgcataacgc ttgtcaggat atcttttaag
    24961 cattaaactt atttgttcac aaaaactttc ataattgtat agtcctgtta atgtatcttt
    25021 ttggttttta tgtagttgca taagataatc tatatttttt atagcaacta aaatatgtgt
    25081 ttgtttctca cctaacttat ttttactact aatctttaac atataaatta atgcccaata
    25141 gtacttttta tctattccta aacgtctata ctcaaaattt agttctaaat cgtcttgctt
    25201 gctattatta agactatcaa gagattttag aaggtggtta tcattaaata atattatatc
    25261 atcaggatga attaaatgtt tttttatgta tgaaaaagtt ttttcaagat tttcctttat
    25321 aggaaaacta aaaatattgc tctctgattg ataaattgga gtaattaaat tcgtatcaat
    25381 gcaaatagat aaaattatat catatgtatt ataagctaat ttttcataag ccttagaata
    25441 cccttttatc attaaatcat caaaataaac cttatcaact aactctttat gtttaaataa
    25501 aatattattc gtcgaatcag ctaagtcact aattactttc atttcattat ttctaagttt
    25561 gttcttatta taatacctct tcttatcttg gtacattctc tcatcagcaa ctttaattag
    25621 cttatgtata tcaatattat cgtcactcca aatactgccc actgctacac tatatatcat
    25681 ctcatcctta aattcttcac ttaaccaact cactaattct tcaaatagtt gttttgatat
    25741 atcttcacat aatacaacaa actcatctcc tccaatacgg taaacattat aagaatgaaa
    25801 gtattttttt attattacac atgctgacac aatagcagaa tcgccattag catgtccaaa
    25861 gttatcatta atatctttta acccatttat atctacaaat acaactccta tggatttaac
    25921 ttttcctttt tgaaaattat ctatatactt aatatattta tttctattat atgcaccagt
    25981 caagaagtca tagtagctca tataatttaa tttattttct acttttcttt ttttcatctc
    26041 agttaataaa aaataagata gggatgtcaa aagagaggta tctccaacag ctttatgtgg
    26101 attatcaact ccaataaacc ctactacttt atcatcttgt tttagtgaag atgtcattaa
    26161 actttcaatt ttttgaggtt ttaaaatttc atattctatt ggctgttctt cttttatatt
    26221 ttctatatca tcaattatta caaaaccttt ttgttcgaat aataaaaacc aattctttaa
    26281 ttcattaata tcaagatttg ttagtgcctg tttttgttca tcaacacctt cattgcacca
    26341 ttcatatgta ttagttactt tttttctatc ttctgatact tcaaaaacat atgctctatc
    26401 agctttatga aatttaccaa tattggttaa tacaagattt acagcatcag aaaaattaga
    26461 ttctgttata agatgtttaa tacatccaac taataactcc tttatctcta atttttctgc
    26521 tattgactta cttatatctt ccttttctgt aatatcaaga gctatttcta atcgtgcatg
    26581 tttgccctcc catttaatta acttgtcctt taatataaaa tgtctatttg ttcgagtatt
    26641 cgtattttcc catatgtaat attcatcttc ttttaaaata gagtttgtac aaaattcgca
    26701 gggagaatct ttcccctgta atgctttata acattttaaa tgggatatat cattaagtcc
    26761 cattgccttt ttcccaattt cattaatata taaaagttca tatgtatcta aagaagaaac
    26821 atatacaatt tcatccaact catctattaa atttattaca tccatttata tatatctcct
    26881 tttattcatt ttccagattg aatttttaaa gcttttaaaa ttttgctata tatttggcaa
    26941 tgatattata tttctttatg ttttaaaata gtaccttaat gaggttctat tttttagtaa
    27001 atagaattat tattgtgtca atcgtacaat agagttttgt aatacctaat ttaatatatt
    27061 tataattaat tatctcatta tgtgtttata attaattctc atataaatga caaatctcca
    27121 cctcaattaa gtattccaat catatatgaa gataatactt cattcattta aaaatataaa
    27181 actattatat attaaaattt cttcaataat aaattatcta gattgttact ttgatatatt
    27241 atataatatc acaaaggctg atataaaaag acaaaaaggg caattttttt atatcaaaaa
    27301 aaaatgttaa attgatatta tccatatata aattgatatt atccatatag atgctatatt
    27361 taacaattct acttacttcc cttacactta ttagcatata gttattaaga gtttgttgca
    27421 aaacaacact tagcacactt taaaaagtgt ggtctaataa gtaatcaacg aagaagacat
    27481 aataaagcat aaaatatgat atacttttcc acgccatcaa aactgaggat tttattatta
    27541 atatatgcta aaatatgctt aataaattaa attttaatgg gagaatagtt atgaaaatat
    27601 ttagtttctt gttgtttgtg ataggaatat gtttatgtat tgctggattt ataggacatt
    27661 acccagtatt tgttggtaca acattgagtt ttcttgcaat tgcaatgata gctgtaagac
    27721 aagtcaaaac taagaaaaaa atgaaatagg caaatcctaa ctttacaagt taatattaca
    27781 caagtaatta gtctaaaatt ggatactttt tctattacag aaatacatca tccactttgt
    27841 attttcttaa aaaaataaaa tagctatgta aattcaagat tagatagatt aactctctat
    27901 ccatttcatt gtaaaatata catagctatt tgtattcgat ttaaaatact tagattttct
    27961 tacataacaa ataatttcta tctgattatt actcatattc atttagtaac cactcagcaa
    28021 ctaatcattt agtatctatt catccgccat acggtaacct accccaacct cagtcaaaat
    28081 atattctggc tgagcaggat tcttttcaat tttccttctt atacttgcca taaaaactct
    28141 aagcgactga ttttcacttt catagtaatt tccccaaact ttatctatta taaacttatg
    28201 ggttaagact cttcctgaat acttagacat caactcaata atcttatatt cgattggagt
    28261 taaatgtatt tcttcaccat taattgacac aatatgattt tcataatcta tctttaggtt
    28321 tttaacttca aaagactttg gagcatcatt atttatatta tcctgttgag ctttatttct
    28381 aagagccaca cgtactctag ccaaaagctc tgttacacta aaaggtttag ttaaataatc
    28441 gtctgctcct ccatctaaag cttccacctt atccctctca tgctctctcg ctgaaacaac
    28501 aattatttta ttagtctttg cacatgccct aactcgtcct attacctcaa tcccatcaat
    28561 atctggtagt cctaaatcca aaattatcaa atctgggtca agagaatgta taagtgatat
    28621 agctgttcct ccattatctg tctctacaca cctataattt tgagtttcaa gagatacttt
    28681 tataaattta caaattggct tatcatcttc tacaattaga actaatggct ttatttccac
    28741 ttgtctttcc cctcctcatc atacataggt atgctaaatc taaatgtagc tcctccagaa
    28801 ggattattaa aggcgacaat ttctccacca tgagcttcta ctatagattt acatatctcc
    28861 aatccaagac caatacctct tctttcaaga tttctagact tattcttagt ataaaaccta
    28921 ttaaaaatat tcccgatatc ttcatttggt attccttttc cattatctgc aacttcaaat
    28981 agtacttgat ttgactcttt ttttacagtt agttctatct tagaattttt aggtgtatgc
    29041 cttatagcat tatcaattag atttactaac acttgttcta ttaataatac atccacatga
    29101 acgattatta tctcatctgg aatatttgtt tttatgtctc tactattggc aaactttttg
    29161 actcttaata tagattcagc tattatttca tcaacaactt ctggtcgttt ttcaatatcc
    29221 aattttcctt catctattct ggtcatacta agtatattct caacagaatg agttagccaa
    29281 ctcgtatctt catatatatt tttcaataat tctactctag tttctttatc tatcacatca
    29341 tcattttcaa gtattgttga agatgaacct aatatacttg taagtggagt tcttaaatca
    29401 tgagaaattg acctaagcaa gtttcctctt aatctctctc tctctgcctc aagatttgct
    29461 tttttatttt tctcaaataa attttctcta tcaattgcca acgcaacctg tgttgaaact
    29521 gcttctaata gtatcttttc attttctgta ataatatcta tcttaaaaca cgcaacacct
    29581 ataacaccaa gaacatttga ttcttgacct tttataggga aataatatgc actgctgttt
    29641 ttacaatatt ttgtcccaat tccaactggg ttacctgtcc taaaagcttc ttttatagct
    29701 tctttttcta atactgaatg gaatatattc tcacttccat cattgttaca aatataaata
    29761 cttggcttac ctagattatt attctcatct gcaatagtca ctattacagt tctatttaat
    29821 atatttacta aattagaccc acaaaattca ataatttgat ttttattttt tgctttaagc
    29881 aacgttttgt tattctcata gagcaatctt atcatttttt ctctcttatg agaaagtctc
    29941 acttctcttt ttatcctaga agttaaagta cttgttataa ttgctgccat tagcataata
    30001 caaaaggtca taggataatc ttttctataa gctaaaaaag tatataatgg atgcgtaaag
    30061 aaaaaattaa atgatagcac tcctattact gaagaaagta ttccaaaaaa atatccatag
    30121 gttatacttg tagcaaatag tacacctaat atgtatacta gtataatatt agattcatta
    30181 aaacctacct tttcaaaaac tactgatatt ccagtagcaa ttgtcataac caatatagtt
    30241 tttatgaagt ccttaataat tatcttttta ctgagatagc catcttcatc ataatcatac
    30301 tcttttaagt cttcatgttg tagtcgttcc tttttcattt actacaattc ctccttattg
    30361 ttactgttta aattggctct attttatata atacaatttt atcataataa attcaatcaa
    30421 tacatttaat taatgtactt aaattactaa aaggtgctgt cttaaaatga aaataaatct
    30481 ctatttcaaa acagcaccta tatatttaaa ttactaattt agtttttaca tacttaaatc
    30541 aatagtttta ttcaatttca taaattataa tacttcaatt ataaaacatt tctatccaac
    30601 aagcataaat ataaagtggg cacatatacc tgcacataaa ccacctatag tatgcgaaag
    30661 tatcgcttta ccagccaatg gtcttgcatc taaagcatcc atcataccta tgtgtgtact
    30721 taaatatcca ctccaacaca tacccattgc tgtgaatact gctatatcat taggagttat
    30781 agcgcctgat tttataaact ctggtactaa agatattgct gctccaacag ctcctaatgc
    30841 agttacaggg aatgctattg cctctggtga tgtgaatcca aataatggtt ctattatgaa
    30901 gctaatctta tcaccaatag caggtaataa ttttatacct tcatatgcag ctcctgtgta
    30961 aacagcttga cctgtagcag ggtctgtaga tggtccaaaa gttaataaca taactaatgt
    31021 acaaactact aaaactcctg gtattatagc catacccatt tcaacaccta acttaccacc
    31081 ttcaagtata gcatccaatg ttctttggaa tacattacca tctcttattt ctctaaactc
    31141 ttcaagtttt gcacctttat catttatttg cttttcagta tcatttttag ggtcataatt
    31201 ataatacttt ttagtaaaag tcaacataat tctaacacta ataatactac ctattattgc
    31261 cgcaacatta cctattatag ctggtagtac atattcttta ccttgagcta tcataaaagt
    31321 tgttacaata agtcccatac caaaggctgt tcctaaatta cataaagcag gtacttgata
    31381 cttcttaaaa tattgtgtaa atgttttgtc tttagcaaat ggtataatag ctggattatc
    31441 agataaatat gttgcaacta ctcctgctat actagcacca ggtaagcccc atataggtct
    31501 cataagaccc ttaaatatct tattaactaa agatattact ccaaactctg aaagcaatgc
    31561 actaagtgca cctgcaagaa ctgccatagc cattattaaa aatactgtgt ctaataaaag
    31621 cgcatgtgct gtactcatta ttactttaaa catcattcct gcacccatga tgcttcctac
    31681 atatccaaat cctaccgcca atagtacaat aaatacgaag gtttccatac ttactgcctt
    31741 gactcttctc ttatccgaca gcttttccat cttgaaatct tccccctttt aattttaaat
    31801 taaggttcct aacattggta caaatttttt ggtacaaact gagttctaaa acttacactc
    31861 gtaatgcaag ttgtcagttt tttgaaataa aaaaaaatta cgtttataga ataagaatat
    31921 cctctttaat ttggatactc ttattctatt ttatgttatt atattatcat ccttttgttt
    31981 aaaagtcaat tttttctatt tttgttgttt aattttattt atactctttt tatatttttc
    32041 attttctttt atctttgtaa gtactccata taagttgact tttctaaata cagtagttat
    32101 aaataccaat cctaatgcaa ttgaacttgc acacgataat gtactcaccc catattctac
    32161 agcttttata taattattgg tcaaaacaga atacatagta ttatatattc cataacctgg
    32221 tactaatatt attaaacagc aaacacttaa tgtagttgct ggagtcatat aaattcttgc
    32281 acataactca caatatatcg caaaacaaac tgtagctata aaaaaacata agttttctgt
    32341 tactcctata tttaaaagaa atttatatga aaaccaagct atacttccac ctattcctgc
    32401 tagtattaag ttttttcctt tcatattaaa tagcactcca aagcttaagg cagtaaaaaa
    32461 agctgctatt acttctataa acaaatgcac ttatatcccc cctaacttga agtacaatcc
    32521 aagtaccaag aacattccta ctgctataga tacacctaca aaaaatactt ctgctaattt
    32581 tgcaagacca gaaatcaact gtccatctaa caaatctctt aatgcattag tcaaagcaag
    32641 cccaggaact aagagcatta ttgctcctgc tattaaatgg tctaacgtct ttatgaatcc
    32701 gagctttaaa attaaaattg aacaagaagc tataataact gctccaattg catttacaaa
    32761 aaagtcatta agggaagatt tacttaaata cataataaca aacttagtca gaatcccaac
    32821 agtaaatgca gctataaatt cttccacatt tcctccaaat aatatcgaaa aagttgctgc
    32881 tgcaatacct gcaaaaaata ttgttattaa atctgagtaa tagttatctt cacttatttc
    32941 atctaaaatt ttctcacata attcaatatc taaattattt atacttattg ctcttgataa
    33001 tgagttaact tcatgaacca agtttaggtc tactcttctt gagcttattc ttttaattgc
    33061 tgaatgtaat ttcccatcta caaatacaga tgccattaca gctgttggag ttgcaaatac
    33121 atctacgtgg tctattccaa aagattgaca tattctagct atcgtttctt caactctata
    33181 ggtttctcct ccactttgca acatagcttt acctgcattt gaggaaaagt ttaaaattct
    33241 atctatatcc acaaaacata tctcccattc ttttatagta ttattcatat tttaatacaa
    33301 gatattattt atttaaacta ttttaaataa ttcatatcca atatttacat tttatgaaaa
    33361 aacttgtttt ttatgcttgt caaaatcaaa ttttaagctt gtttacacca taaaatggct
    33421 gaattaatat ttgacaaaca ctttcaacca tttatataac cttctaaatt gtataacttt
    33481 ttactacaaa taaaattata tctcttttaa ttctttctct tgagtattta gcttatagta
    33541 acttcatatt tatatatgta caatataaaa cattattttt atgatacaaa atataatttg
    33601 taaatgtata tatgtattaa ctgtgtatat ataaattatt cttgacctta tcagtttttt
    33661 catatatctt catctatacc aaccatatat taataaatga tattacatag acttaagtat
    33721 attaaaaaac ttaaatttag ttatttaaca acataatact ctaatttaat ctataaaaat
    33781 ccatacttaa tataatgcaa ctcttatttt aacaccatct attacacaaa acaacatatt
    33841 aaaaagtaaa atacaaaata tccactataa accttatttt aatactgaat atatattata
    33901 atatatttaa tgtagcttaa atatagttta aacataaaga aaggaaacgg tatatgctaa
    33961 gaaaagataa agttctatta tctctacata aattatgtga aaatatgact ttaaaagacc
    34021 ttcaatcaga taaaacagga ttcactacaa tagaaattgc taaccattca tctcttgata
    34081 gaagtaatac cagtaaagaa ttaaataacc tatacaatga aaaaaaagtt ctgaaaatat
    34141 ctggtaagcc aattttattt ctacatgcta gtattataga aaatttaatt ggccgtactt
    34201 tgaacagtga tgaattttct ataaataatt gtaatgagct acttaaatca agtaaaaatg
    34261 actcagaaga tattttttca acattacttg gacatgattc tagtcttaaa ctacctatag
    34321 aaaaagctaa atctgctata ctctatcctc caaaaggtct taatacgtta ctaataggac
    34381 ctactggtgt tggaaaaagt acatttgctg aaacaatgta caaatatgct gtagaaagta
    34441 aaatcttttc ttctgaaagc aaattcgtag tatttaactg tgctgaatat gcagaaaatg
    34501 ctaacttatt attaagtaat ttatttggtt atgtcaaagg ttcttttact ggtgctaata
    34561 aggataaatt cggcatagtt gcacaggctg atggaggcat attattctta gatgaaatac
    34621 acagattgcc accagaaggt caagagatgc tttttctttt gatggacaaa ggcatataca
    34681 gacgattagg agaatctgac attatgcaca aagctaatgt ctttattgtg tgtgccacaa
    34741 cagaagatat acattcttct ctgcttggga cttttttaag aagaatccct gttacaatca
    34801 acctacctgc tttacaggat aggtctttaa aagaaagatt atctcttatt aaatacttct
    34861 ttattataga atcaaaatac accaatgcaa ctataaaagt tcacaaagat gtaattaaat
    34921 cattactcct ttatgattgt tttggtaata taggtcagct taaatctgac atacaactta
    34981 tgagtgcaag agccttctta aattacaaaa ctggacttaa ggataatata gaaatagatt
    35041 tatcccttgt tcctgattac atctatattg gtcttttaaa attgaatgaa aatcgtagta
    35101 atctaaataa attatcagaa cttgattata tgctttttta tgaatttaat ggtaataact
    35161 tagaagttga atatacaaaa gaactatcta aatacaatat agatacatca aacaatggat
    35221 ttaaatactc tacagagatt ccttatctgg aaaatgatat aaggagtatg tttataaatt
    35281 acatggaaaa gtactctaca aaattacttt cgatatcttc agtaactcat tctgattata
    35341 caagtaatga gatttttaaa gtaatcaatc caaagattta ctatgctgtt gaacattctt
    35401 taaaagaggc tgaaaaaata tcgagaaaca aatacacaaa acaaacttat attgctcttt
    35461 caatgcatat aagtgcactt atagaaaata taaatcaaga cacatataat cctaagtaca
    35521 atttatataa tgataaaatt ttcttagata aagatttaaa tatagctaaa atagttttaa
    35581 gtataattga aaatgactta caaacatctt tacctaaaga tgaagtaaaa tttatatcca
    35641 tgtttctaaa tagcatgtat cttgaaccaa acaatcaatc tgaaagtata ggagtcataa
    35701 tacttgctca tggaaattca acagcaagca gtatgtgtga tgtagcaaat tcattgcttg
    35761 gtacaaacca ttgtaatgca attgacatgc ctcttgatgt aaaagtaagt accatacttg
    35821 ataaaactat agagttggta aaagaatgtg acgaaggtaa gggtgtactt cttttaacag
    35881 atatgggctc tcttatagct tttggagaac ttatttcaga aaaaacaaaa atcaaaactc
    35941 gctcaattga aatggtatct actccaatag ttttagaagc agtaagaaaa agtacactac
    36001 cagaaatgac tttggatgaa ttagctaatt ccttaagctc tttaaatcca tatattggac
    36061 gagcagcaat cgattcaaca gaaataaact atcctgaaaa gtcaagagag tatgtaataa
    36121 taactacttg tataacagga tatggttcag ctatgaaagt agcagacttt ataaattctt
    36181 cacttaaaaa tattagtaaa tacaatatta atttagttcc tcataatata gaaagtttta
    36241 aaatgtataa gaataaattt agtaatacta aaattctggc agttgttgga agtgtagatt
    36301 ttaatgtagc caacacccct tttatacaca tagaggaact actaaatgga tatggcctag
    36361 atatgctaaa caaaataata gaaggtacat tcaatgccaa tagtattaca aatataaaag
    36421 aaacaaatct atcatacaac agtttaataa taaactttat gacccaaaac ttagagcttt
    36481 tggactctgt taaattattc aactatgtca attcttcctt atttacaata atagaattaa
    36541 tagatgaaaa cttaaagagc aaatttaagc taggatatat actacattgc tcttttatga
    36601 tagaaagatg ttttaaaaat acaacttttc catataataa cattactgaa ttaattagta
    36661 aaaatcatga actatatagt ttaatacgtg aagcctttaa gccaacagaa gcttatttta
    36721 atattttgat accagacgaa gaaattgctt atataataga catgattaat gagtatctaa
    36781 ataaataaaa tttatagcac ttattttgtg catattaatt aatatatgaa atggaggacg
    36841 agattttgaa aggaaaaata aaacagttaa caatacttgc attaattttt atatttataa
    36901 ctcctgtatt tgcatttgca gatactccac cagtaccaaa ttcttcaaga gcagcactac
    36961 ttatagacca ggaaactaaa agaatattat ttgaaaaaaa cattgatgaa aaaatgccat
    37021 tagcgagttt aagtaaaatg atgacctttt tattggcaat agaagcagtt gataaaaatc
    37081 aagttaaaga gactgatatg gtgaaaatag acaagtctac agcttcagta ggaggttcta
    37141 cttgtaaact taaagatggt gatgaaatat ctctaggaga gcttatgcag ggtctaatgc
    37201 tagtatctgg aaatgatgct gctatagcca tagctaaaca tattggtaaa actgaaaaga
    37261 attttgtaaa tatgatgaac aaaaaagcag aagaaatagg aatgattgat acgtattatt
    37321 ttaatccaaa tgggcttcct atatatactg acccagaaca taaagaacct cctattgaaa
    37381 atatgtcaac tgctcacgat atagttacac ttggaaagta tatgtacgac cattatgaaa
    37441 atcaagttac aagaataacc actatgcaag tatataatga tactaaaaag gattttacac
    37501 attataacac aaatcctctg cttgtttcag tacctggtgt tgatggaata aagacaggat
    37561 atacagataa tgctggatat tgccttgcat tttctatgat ggtacctaaa gatgctaaaa
    37621 acgaaagaaa tcatagattg ataggtgttg tacttggtga tggtaacaag aaaaatagaa
    37681 tttcttcttc cgctacttta ttgaaatatg gtaaagataa tttccattct aaaaagatag
    37741 cacataaggg tgatattata gagactcctt gtgttgatgg tattgatgat tttaagataa
    37801 cagttaaggt tgataaagat ttgtatggtg ttgtttcaga taacgaaaat atcaatccta
    37861 aggtagtgtt taaaaatatg aattatccta tccataaggg agatattgtt ggtgttgcaa
    37921 agtattataa tgactctggt aaatttgttg gaagtgttga tgtaaaaagt gaaagtaata
    37981 taggatgtat tcctttgaag gataagatta agattaaggt tgctaagata aataaaaaac
    38041 ttgagattaa aaattctgtt tgttttaaag catagtaaag catattatta aaagggtatg
    38101 ttaaatttat aaagactcca ctttgtggag tctttttgtt ttttacaaaa aattaaaata
    38161 gtggcaacaa ttgaggatgt gagggagagt tgccactatt ttgtataggg tattatatta
    38221 tgtttttaag ctagttttac tatcttctat gtttatataa taactcttgt gtgtgaattt
    38281 aattgataat taatatgaaa attatgtgaa gttttgattt atttgtaata tattctacta
    38341 ctattataat ttttctactg gatatactaa tcatttgttt aacattatct gagattgatt
    38401 tgcaaacttt tatataacta aattagttat ataacttata gattccatta aataccctaa
    38461 taatcatata tgtacataca aagtcatgct tatgcaaaat ctgcagtttc aactaactta
    38521 ttatataaac catctttatg tagaagttca gcatcatttc cagattctat tactctacct
    38581 ttatctaata catatatatt atcagcactc ttaattgttg aaagtctatg agctattata
    38641 ataactatct tatcttgtat gtactctttc aagttgttca atatctcttt ttgtgaaata
    38701 ttatctagag ctgaagttgc ttcgtccaga ataagtacct ttttattact tactaaagct
    38761 ctagctattg atatacgttg tttttgacct cctgatattt tgacaccttt ttctcctact
    38821 acttcgtcca atcccttatc tagatttttt ataaaatcat ctaaacaagt aacttctaat
    38881 atctcatcta attctttatc acttatttta ttatttaata ctatattatt ttttattgtg
    38941 tcatcaaata caaattgatc ttgagttact atatcaatat tatttcttag tgattttaaa
    39001 ttaatatcct ttatattaac accatctatt aatatatcac cttcatttat atcccaaagt
    39061 cggtatatta gatttgttat tgttgatttg ccacatccac ttccaccaac aattcctgtt
    39121 acttcccctt tctcaaattg taaatttata tcatttaaaa caaggtttaa atcctcacca
    39181 tatctaaaag atacattttt aaaatctatt ttcttaattt cactgttatc tattctatat
    39241 ccattattat caactttaat atctatcttt tcatctatta ctgaaaatac tctattgata
    39301 gacacttttg attgtgctat tctagtattc gcccttacta tagctataca tggtccaatt
    39361 aacattcctg tatattgttg aaatgctatt aactccccca tcgtcatctt attatttatt
    39421 attttatatc ccccaattcc atatattaat attgttatca atcctgttag tatttgagat
    39481 attgctatat tactactcat aatgatgttc aaattcaaat ctttttttat caaatctctc
    39541 tctctcttta aatacttatt aaagaaattc ttttttgaat ttaatataac aatatccata
    39601 atattagata cgtactcttg aatcaaattt gataaactgc ctgaatcact tcttatttca
    39661 gaaattttct tagatataag ttttgtcatt ttattttgag aaaatgttaa tatcaactgt
    39721 aaaaataaaa ctactaaaaa taaatcaaac tgcaaattaa ttaagaaaat taacgatatt
    39781 atagctgtaa acacatttac aataatatca aatataatct ctgctccaaa gttttctatc
    39841 ataaatatat cactttccat aatggataaa atattacctg ttttcttact tgaataatat
    39901 ctaccagaaa gttttgatat atggttaagc aactttactt ttagatttat agtaacttta
    39961 ttttttattg aagtatataa atatgataat ataacatcaa atgtagctga taatatcctt
    40021 ataaaaatat atatcaatat taaatttata agcagaaaaa tatttttatt tcctattcca
    40081 tcatctatta tacatctaat tagttgaggt attacaatat ttacgccagc aattataaaa
    40141 accattataa atgatactat caacttagtc ttattcttat atactattat atttagtatt
    40201 ctaaataaat tattattaat agtttttttc atctattttt ccatctttta tagttataat
    40261 tctatcagct gtctttgcaa tattatcatt atgagttatc ataactagtg tttgtttgta
    40321 ctctttaaca caccattcaa gcatattcat aacttcattt gtagtcttac tatctaagtt
    40381 gccagtaggt tcatctgcaa atattactga cggtctattg gctaaagctc tagctattgc
    40441 aactctttgt tgttgacctc ctgatagttc attcggatat ttctttattt gtttttcaat
    40501 ccccaactta ttaattaatc tcataacata ttctttatct tcttttttcc catcaagcaa
    40561 cacaggaagt actatattat catatacatt tataactggt attaaattgt aactttgaaa
    40621 tatgaagcca aattcctgtc ttcttatttc agacagctta tcatcattta atgtatataa
    40681 atctacatca tctaagtaca cattccctaa agttggcttg tctaagcctg caacacagtg
    40741 aagtagtgta cttttaccag aaccacttgc acctacaata gctgtaaatt tatgtggctc
    40801 tatatctaaa tcaataccat caagagctct aacttcattc tcatattttc catatatttt
    40861 ttttaaatct tttaccttaa gtattttcat aacgtatctc cattcccatt taaaaaacat
    40921 atatttttaa attaaatttt cgtaaataag atctactgcc atatctaaat tctctaattg
    40981 atataagtat acttctttgt agtcaatact aatattaaac tctttattta aaatagttaa
    41041 aaaattatat gcattataat ttccaataca tgtaaaattg ttaaataaag aatattcact
    41101 tacaaattca aagtctattt ttattttaga aaagtttcta tttatcacat ttattatttt
    41161 ccttgctata tccaatctta tcaaattaga tttataactt ggagtgttca agttttctat
    41221 ttttaaatct tctttacttt tgcttttttg aataagtatt tcttccaatt cattaaactt
    41281 aatatatgga ttgtttttta atatatttgc aacaatgcca gttgccactg cacaagcctt
    41341 actattttta ccaaaatgag ttactttatc tttaaattta ataaaattat cttttgaatt
    41401 tgcatacatt tgaatcttat tttctggttc gtatatatat tgattatttt tatatatatt
    41461 ttcattacct actacaccaa taacactttc aaatatagta ggaatacttt ctttatcttc
    41521 atatttatta tgttttgaac aaataataaa tctaccttga ctatttaaaa tattacaaac
    41581 cttttctaat tctttttcat acatactatt aatagatgca gctgaaatgt ttattatctg
    41641 tatatccgac atagtcaact tgctcagtgt actaagaaga tttaaagaac ttgtttttcc
    41701 agaatcatta aatatattaa taggataaat ttgggtattt ttacttatat ttaatatggt
    41761 tttcgataca aaagttccat gtccatttat gtcatttatt tccttccccc ccaaatcttt
    41821 caattgaaaa tttgaatcat aaacataatt tccattaata tcatcatcat ttacatctaa
    41881 tcctgtgtct aagactgcta ttcttacttt agtcaattaa taatcacctc taaaataaat
    41941 taccttgaat ataaaactaa tccttaccat aatataggtt ccaagatgtt tttcattttt
    42001 ttacaatttg catcaaacat ttttttgtta ttttttaaag tatatatttt cgatggacaa
    42061 gttgtacaaa ataaatttac tttacattct ttacaactct caatatttac tggttttaat
    42121 ttatcaaata tattaaatat atccatttct ctattaagaa tcctctctaa tatgttttca
    42181 ttaaattcta atatattgaa tatctttaaa tccttatact ctaagtttgg acacggatat
    42241 acatctcctt tatgatctat acttatctga ttaacacctg ctttacacat atttgaacat
    42301 aaactacttt catccacata atcatctttg caaatgtaat caatatcaag tttatcatta
    42361 agatatttgc tatagttctc attacctcta ccaatattca taaaatttct tattataggc
    42421 gttgtaccta aatcttcatt taatttattg aatttctcta tgtctttctc attattcttt
    42481 cctaccacca tagacaaacc tacgttctcg aattttttta gttttataag tctaatcaca
    42541 tttaatactt tacagaatac tcctttacct cttacttcac tacaacttaa ttcatcataa
    42601 ccatctaaac taatttctat tgcatataaa ccattaatta tatcatcaat ctgtttttct
    42661 ttaattaaag ttgcatttgt agacagaatt attttacctt taaatttttc ttttatataa
    42721 tccaacagct ctaagaaatc atttctcatc aatggctctc cacctgtaat ggttaatatc
    42781 cttggattta atttaactat attatctatt acttttctta cttgagatgt atctaaataa
    42841 tcgttattat ctattttaga atctacacag caataatcac aacttagatt acatctatta
    42901 gttggagaaa atgaaatttt gtttattctc tctagtctaa attcacttag aatagaatca
    42961 cttagaagtc ctatatgctc taacttatct ataactcgtt cataatattt tttatcttca
    43021 ttttcttgga atacatcata caaattacca agttgtaatg cattatctac tgaatacttt
    43081 agaacttcat aacattctat tggaatttta acccatcttc catttacttt attactaaga
    43141 ataacttgat tattatttat tattttttca catatacaat ctaaaaaata tttgtttgag
    43201 ctattcattt attttatttc cttcctatat atttaaatta gaaatttcat taattttctt
    43261 ttataccact tactatacta agtttattta tcattttatt agaaaaataa actgcaatga
    43321 cacaaacaaa aacagatata gatccaaata ttagaatttc atatattgga atttcaaatt
    43381 taatagagtt atttatacca tatacctgtt gattaagttt gttagtttca gaaactattt
    43441 tataataatt gaaactagat ataattccag ctacaatcat acttaatacc ccatatatca
    43501 tattttcttt tattaacatt ctttttacat tttttttgga catccctatt gctctaaatg
    43561 tacatatctc actcaacctc acaattatat ttgatctaac tataaaaata atgtttatag
    43621 atgatattaa taaaactcca aatacacaaa tcattgctaa tttatatgag gatttattat
    43681 aatggtctcc aaaatgatta cgtacatatc ttccctctat atctgaaaat ccactttttt
    43741 ctattatatt atttatttct tttattactt ttttatctgt ttcttttttt atcttcacaa
    43801 aaactttatt ataatcactt atccctgtta attcttttaa atccttttca ttaaatataa
    43861 tttgaattcc acctgattga gattctccat cttgctctga tatatagcta tttttcatta
    43921 ttccagcgat tcttactttt gtatttatat atttatactc tccatcttta tatacaggaa
    43981 tttttatatc tattaagtct cctattttag gtgattttat aatctgagta ttatttgaca
    44041 atttaattct agaataaaaa ttatttacca ataaaacatt tttataatcc ctgtctgtat
    44101 aactataaac attttcgcct ttatctatat atttatctat attatttaat agctctttat
    44161 tgtaacctcg aattaaaact gtagactcat tattattatt tatttcagtt ctttttagct
    44221 catctaaata atcttcttct aagtcagcat ttcttagata tccttttaaa taaatattgg
    44281 gttcaacatc tgttacatct tcaatagctt ttatttcatt taataaagaa ttatcatatt
    44341 tagaaaatgt ataatttgca tttattggtg aatatcccaa ataaatatca aaattgccat
    44401 atgatttact tgttatatta gctgaatctg actcttttcc atatatgaat tttgtagaag
    44461 ttagtctgcc tataaataaa accccacata gtgatatcgt aattattatt attattgatg
    44521 taaccttatt cctaaatata tttttaaaag ctatctcatt tataattcca acaacttttt
    44581 tatgtgattt taacaacaaa agcctaggaa tataatttaa cttatctgtc ttttttatag
    44641 catcaattat agataaattt ccagctttat atattggtac tacagaggct aatataactg
    44701 ctataattga catgatgata gattcaatta tagagtttgt atttatagtt aattctgcac
    44761 ttttatcata tactatcatg agacctaagt aagaaaaaac tatacctcca agtaaaccaa
    44821 ttactatacc tacagataaa actacaatac tctgaataaa aaatacctgt ctaatatttc
    44881 tcttgctagc tccaacagct ctaagtaaac caatttgctt ggtcatatct agtattatta
    44941 tgttaaacat attgtatatt actaatgttg cacataataa agggagtaag ctttgcttta
    45001 ttgaataagt tgtttttttt gaaatatttt tcatatacat agacatactt acttctgtat
    45061 ttggataaaa ttgattactt gttaacttat attttctccc tagattcact actttttgag
    45121 aatctaaatc ttttgtattc aattttaatg tacccatatg agttattaat tcatatggaa
    45181 caatatcttt gccattgttt gaatatgtaa aagctcttac cttatattta tttaaatttt
    45241 catagtattc atcagattta cttaaaatac caactatttt aaattgctta tctttactat
    45301 aaatagaatt ttcattattt ttatttatat gctcattaac aatctgaaaa tcaatagttt
    45361 gatttaattt gacatctaat tccattttgt ctaaaacttt ttgttctatt actaattcat
    45421 tagaagttct aggctctcta cctttaacta acttatatct agtagaatct aaaaaatctt
    45481 tattgtaaga gcataaattt agagatgctc cattatcttt acttataatt tctccaaatt
    45541 taagtacatc attgaattct tttatatctt cttcaccatt aagttctttt actatatcac
    45601 tatttatatc ttcataaaaa gcatgataat ctcccgagtt tttgtatgca tgatttatat
    45661 ctgattgtcc ttgagattca ttaataacac ttaatgaaaa aactagcata acagcaatcc
    45721 ctatactaag tattaatgat aaagtttttc ctttttgtct ctttaaatat gacaccgcaa
    45781 gtttaatatt tatcataatc agaatcctcc ttattcatat ttacaagaaa aactctataa
    45841 cctaaaatag taggttatag agcactattc attaaatact aactaactcg ttgtgctagt
    45901 taaatttaat tattaaaaat actagagttg agaaaatcac atttcatttg taaaaaacta
    45961 catctagtac tttcaacata tttaatagac tgattagtat tttttaataa gctactgttc
    46021 cttttggagt tgatcctgca caatccacca tacagtctat ttgtgaaatt attccatgct
    46081 tatcataata ccatttacat ggatctgctt gcacctcaac atcttgaagt tctatttttt
    46141 cttcgatttt ttttatatgt atcataatac atatccccct ttacttttgt tttatattta
    46201 ttataactag ttaaactaga ttataaacta catagttttt ttgttgtttc cacataaact
    46261 ctttctaatc cacaataaat tggtactgca ctttggaatt tacctgttac ctttttattg
    46321 ataagtttac atctggttga catacaataa tcaagtctag tacatccatc acactcttca
    46381 ttcttagata tataaatttt tataaggttc ttaatttttt tatcatctaa tccatcatat
    46441 atatttccta gtaaaatatc tttatctcct acacatactg tacatggata tacatctcct
    46501 ctagtatcta ttgcataact atttatccct ccaaaacaat ctcctttttt cttatttata
    46561 aattgtttat ctaaaataga tatatctata tcaggataat cctttttaat attcataatt
    46621 ttactaattt gttcatttaa tattttaata tgagaattat cccaattttt atccataaaa
    46681 tctgaaattg gtgcgatatt tttaaaccct tgttttgcta tatcatatac actctcataa
    46741 agatacttaa cagtatttga gttatatgtc attcttgccc taacatatat acctttatct
    46801 ataagtcttt ttccattttc cattactttt tcataagtac ctactccgtt agcatctatt
    46861 ctatttatat catgtatatt cttttttcca tcaatactta tcgatatgtt ttttacattc
    46921 tttgctataa actctattat ttcatcattt ataatagttc catttgtagt tatatcaaat
    46981 aatatctttt tgtcactcaa tctatctact aaatccctat gtatatattt aatcaaatca
    47041 aaatttataa atggttctcc tccatgtaaa acaattgttg tataatcact attactttct
    47101 aattctttat tacaaataaa atctattgtt ttatcagcag tttcataact catattttct
    47161 ttttttttat cattaccttc ataacaatat gtacatctca aattgcagtc ttcagttaca
    47221 aatatagtaa atttcaatta actttttcat ctccccatac agctgtattt aaattgtgtt
    47281 tcatcatact acaccatcta tccaattcca attcattatc cttaactgaa tctaatatcg
    47341 ctgggcaatg ccaacaaaaa atatttacat cacaatcttt acatttttca aagttatatg
    47401 gataaatact ttctaattta atcaaagact ttagatctct tgttttattt tcttttataa
    47461 cttttacaaa ttcattgtca aatatatttc ccattttata gtcatccttt atcagtgaag
    47521 gacaaggata tacatctcca tcataattta taaatatttg agagtttcct gcattacatg
    47581 cacaacatcc cattttttta ttttttttat ctttacacat ttttaattca ttatctaaaa
    47641 tatacggaat tgtaattgga agaactgtat cttcaattaa atagctttct ttattttcta
    47701 ggcctcttcc tgttggaata aaatatcttt caacaaattc tgttccaagc tttttattta
    47761 aatctttaaa ttgtttattt aaatgagaat ttttttgtcc aaataccata gacagcttta
    47821 tattactgaa tccattgtct tttagcagat taacagagct tattacttta ttaaatacac
    47881 cttttcctcg tgtttcacta caagtatttt catctactcc atcaatacta atatctattt
    47941 tttcaaaatt agttataata aaatcaacat tatgttcatc tattaaagtt ccatttgtgg
    48001 ataatattaa tttggtatca ggtaattttt gttttatata tttaattatt tcaaaaaaat
    48061 cttctcttat caatggttct ccaccagtta taattatatg aggagtattt aaaactgata
    48121 ttatatctac gatatttttt aaatcatatg tactaagtaa atctttctta ttagaagaag
    48181 attcaacaca acaatgttta caaaataaat tgcatctatt tgtcaaacaa aaataaattg
    48241 caacaccatt gcttaacata tcaaagttta aattgttact tttaaaaatt tgatttttgt
    48301 tcataatatc tataagatta ataa
//
LOCUS       DAYASV010000015        47606 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYASV010000015 DAYASV010000000
VERSION     DAYASV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.015"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(237..2516)
                     /locus_tag="ACGI68_000879"
     CDS             complement(237..2516)
                     /locus_tag="ACGI68_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0137327.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2787..4241)
                     /locus_tag="ACGI68_000880"
     CDS             complement(2787..4241)
                     /locus_tag="ACGI68_000880"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFH0137328.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4405..5274
                     /locus_tag="ACGI68_000881"
     CDS             4405..5274
                     /locus_tag="ACGI68_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0137329.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNTLFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5487..7559)
                     /gene="ptsG"
                     /locus_tag="ACGI68_000882"
     CDS             complement(5487..7559)
                     /gene="ptsG"
                     /locus_tag="ACGI68_000882"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-specific PTS transporter subunit IIBC"
                     /protein_id="HFH0137330.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENTLVEKIDSLTNGELLDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVTTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(7862..8704)
                     /gene="licT"
                     /locus_tag="ACGI68_000883"
     CDS             complement(7862..8704)
                     /gene="licT"
                     /locus_tag="ACGI68_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0137331.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(8965..11007)
                     /locus_tag="ACGI68_000884"
     CDS             complement(8965..11007)
                     /locus_tag="ACGI68_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0137332.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVVKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATVLLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(11159..12130)
                     /locus_tag="ACGI68_000885"
     CDS             complement(11159..12130)
                     /locus_tag="ACGI68_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137333.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKEIKCVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(12131..13108)
                     /locus_tag="ACGI68_000886"
     CDS             complement(12131..13108)
                     /locus_tag="ACGI68_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137334.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTIGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            13327..14886
                     /locus_tag="ACGI68_000887"
     CDS             13327..14886
                     /locus_tag="ACGI68_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0137335.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGESKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            14992..15951
                     /locus_tag="ACGI68_000888"
     CDS             14992..15951
                     /locus_tag="ACGI68_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137336.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPAYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLAKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            15954..16766
                     /locus_tag="ACGI68_000889"
     CDS             15954..16766
                     /locus_tag="ACGI68_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137337.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(16982..17884)
                     /locus_tag="ACGI68_000890"
     CDS             complement(16982..17884)
                     /locus_tag="ACGI68_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0137338.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            18239..19417
                     /locus_tag="ACGI68_000891"
     CDS             18239..19417
                     /locus_tag="ACGI68_000891"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFH0137339.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTIAVKEAISRVRLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            19426..20082
                     /locus_tag="ACGI68_000892"
     CDS             19426..20082
                     /locus_tag="ACGI68_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFH0137340.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            20082..20747
                     /locus_tag="ACGI68_000893"
     CDS             20082..20747
                     /locus_tag="ACGI68_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HFH0137341.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            20852..21628
                     /locus_tag="ACGI68_000894"
     CDS             20852..21628
                     /locus_tag="ACGI68_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFH0137342.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            21690..23024
                     /locus_tag="ACGI68_000895"
     CDS             21690..23024
                     /locus_tag="ACGI68_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0137343.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            23520..24452
                     /locus_tag="ACGI68_000896"
     CDS             23520..24452
                     /locus_tag="ACGI68_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFH0137344.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PDNNKPESNKPEDNNNSGSTNKPNNNKPESNKPEDNNNSGSTDKPDNNKPESNKPEDN
                     NNNNSDSTNKPDDNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVAT
                     KKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSE
                     GHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(24844..28383)
                     /gene="nifJ"
                     /locus_tag="ACGI68_000897"
     CDS             complement(24844..28383)
                     /gene="nifJ"
                     /locus_tag="ACGI68_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFH0137345.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPVNT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMGICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(28901..29470)
                     /gene="pduL"
                     /locus_tag="ACGI68_000898"
     CDS             complement(28901..29470)
                     /gene="pduL"
                     /locus_tag="ACGI68_000898"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFH0137346.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(29542..30150)
                     /locus_tag="ACGI68_000899"
     CDS             complement(29542..30150)
                     /locus_tag="ACGI68_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0137347.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            complement(30158..31921)
                     /locus_tag="ACGI68_000900"
     CDS             complement(30158..31921)
                     /locus_tag="ACGI68_000900"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HFH0137348.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNDTIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(31964..32425)
                     /locus_tag="ACGI68_000901"
     CDS             complement(31964..32425)
                     /locus_tag="ACGI68_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137349.1"
                     /translation="MLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKIDS
                     VFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKNNI
                     DISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            32615..33076
                     /locus_tag="ACGI68_000902"
     CDS             32615..33076
                     /locus_tag="ACGI68_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137350.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKV"
     gene            complement(33169..33339)
                     /locus_tag="ACGI68_000903"
     CDS             complement(33169..33339)
                     /locus_tag="ACGI68_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137351.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(33528..33749)
                     /locus_tag="ACGI68_000904"
     CDS             complement(33528..33749)
                     /locus_tag="ACGI68_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0137352.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(33833..35059)
                     /locus_tag="ACGI68_000905"
     CDS             complement(33833..35059)
                     /locus_tag="ACGI68_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0137353.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(35121..36053)
                     /locus_tag="ACGI68_000906"
     CDS             complement(35121..36053)
                     /locus_tag="ACGI68_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFH0137354.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            complement(36142..36669)
                     /gene="hpt"
                     /locus_tag="ACGI68_000907"
     CDS             complement(36142..36669)
                     /gene="hpt"
                     /locus_tag="ACGI68_000907"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFH0137355.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            36898..37974
                     /locus_tag="ACGI68_000908"
     CDS             36898..37974
                     /locus_tag="ACGI68_000908"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0137356.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(38060..39268)
                     /locus_tag="ACGI68_000909"
     CDS             complement(38060..39268)
                     /locus_tag="ACGI68_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HFH0137357.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(39429..39818)
                     /locus_tag="ACGI68_000910"
     CDS             complement(39429..39818)
                     /locus_tag="ACGI68_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HFH0137358.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            complement(39922..40923)
                     /gene="asnA"
                     /locus_tag="ACGI68_000911"
     CDS             complement(39922..40923)
                     /gene="asnA"
                     /locus_tag="ACGI68_000911"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFH0137359.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     misc_binding    complement(40982..41237)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(41371..42561)
                     /locus_tag="ACGI68_000912"
     CDS             complement(41371..42561)
                     /locus_tag="ACGI68_000912"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0137360.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEISRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     gene            complement(42842..43963)
                     /locus_tag="ACGI68_000913"
     CDS             complement(42842..43963)
                     /locus_tag="ACGI68_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HFH0137361.1"
                     /translation="MGTNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGVAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTVVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            complement(44024..44509)
                     /locus_tag="ACGI68_000914"
     CDS             complement(44024..44509)
                     /locus_tag="ACGI68_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFH0137362.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            complement(44511..45179)
                     /locus_tag="ACGI68_000915"
     CDS             complement(44511..45179)
                     /locus_tag="ACGI68_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFH0137363.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            45569..47593
                     /locus_tag="ACGI68_000916"
     CDS             45569..47593
                     /locus_tag="ACGI68_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0137364.1"
                     /translation="MDKKKLILITYDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLELDEKLIKSPSLMEYMPKSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCVEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT        16351 a         7762 c         5749 g        17744 t
ORIGIN      
        1 gaaattacta ttattactat ctaaagtatt ttgctataat cccatatact cttacctcaa
       61 aaacattcaa atgtaaaaaa tactacatat taataaactt tgcccaataa ttcctaaaat
      121 aatatcctaa caatgattta tgtttttata atagtatatt tttacataaa aaagtgaaca
      181 ttgattaaca aagttcactt ttttataaat ttttatgtta taattcacaa ttttatttaa
      241 tttgtttctt ttctatttaa actatatcta tctaatatta atttttcata ttcttctatt
      301 ggcattggtc tagcaaataa aaatccttgg gccatatcac aacctataaa ccttaaaaac
      361 tctgcttgct ctacagtttc aacaccttct gaaacaactt ttatatcaag ttgctttgcc
      421 atttgaacaa tacttgaaac tacaatttga ctcttttttg catttcccac tgttataaag
      481 aattgcctat ctaatttcaa tacatcaatt ggcatatctt taagcatatt taaagaagaa
      541 taaccagaac caaaatcatc catagaaatt ttaaatccaa cagatttcaa agtttgcatt
      601 attttaaata atatttttgt attgtcaaat attgcacttt ctgtcaattc aatctcaatt
      661 aaatttggtt caatttcata ttcttttgtt attctttcaa gctcagaaat aaagcctgga
      721 ttctcaagat gtactcttga taagtttatt gatataggaa atgtggggaa attttcattt
      781 tcccaacgtt taaaattttt acaaacttgg gtaaatacaa acatatccaa ttctgttata
      841 aaaccatttt tctcaaaaat aggaataaat acacctggac ttattagacc ttttttggga
      901 tgttgccaac gtacaagtgc ttcagcacct acaattttag tagttgataa atcaacctta
      961 ggctgtaaat aaactttaaa ttgttcattt tttaaagcat catgcatttc ttgttcaata
     1021 tcacgttctt ctatgagagt ttcttttaaa ttttcagcat aaatctcata aacttttaaa
     1081 tattttcttt ttactgtagt ttttgcaatc atagctttgt caagacaggt agaaatattt
     1141 gtttctccct cttcaacaaa atatacacct atggatggaa ttaaattgag ttctactccc
     1201 aaacttgttt ttatcattga caatttttta cgtataagtt cacatatttc tataattgac
     1261 tctctattat ttttcttttc aaatattata gcaaaattgt catttgaaat tcttgaaaat
     1321 acagattgct ctttaaatat attcttaatt atctctgcta tttcctttaa tactctatct
     1381 ccttcttcat acccaaacct atcatttact agcttaaact tatctatatc aaaataaatt
     1441 aaaactagat tctttttctc atgagaggat aaaaattttc ccccttcaag attaaattta
     1501 tttgaattgc caatacctgt aacgttatct ttaaaagcaa attcttccaa tttttcctta
     1561 ctctttttat gtataaatag tatataaatt gttaatgccg taaagattat aatcaagact
     1621 acacatgctg ataatgtaag ttttataata gcatatgatt tctcagatat agcagtttta
     1681 ggaacttcag aaaaaagata ccaatcattg attcctattg gggcataact taaataatat
     1741 ccttttccaa acatactata acttaatgaa cctgatttaa agttttttat atttgacttc
     1801 atattgttga tactagattt tgatattgaa tcacttctgc caatgtaaga aaataaattt
     1861 tctttagtag agttggcgtt tttactgctt ggatttaaaa caatatcacc attacttcta
     1921 gttatataag aatatccttt tccattaaat gtaggtacag aaagtatttt ttcatacaac
     1981 ttcgtactat gtgtggcaaa tataactcct ttaacctcat tatctttata aattggtaca
     2041 gaatatacat taatattact attggtattt ttatcatctt ctattacatc ttttaatttt
     2101 cctgttataa ccacttctcc ttgcattgat tttaagaaat aatctctttt agagaagtct
     2161 tctatataac catctgtagt gtatgctgta ccatttggta aaataactcc cattctttta
     2221 aatgaactat taatagcttg tctttttagt atagaaagga tattatctac ttcaaaagac
     2281 tcttctcctt caataaatat tgaaattgac tctagtaact ttatatctcc attaatttgt
     2341 ttttttaaca cattgacgct ttgatttgct atttctgaaa gatattgatt cgtttcttca
     2401 cctaatgtct tattcaacct tgaaccataa atcaaacagg aaattgtaat tactaagata
     2461 cttatgacag caattatgga caaaccatac ctattatttt ttgcatcact taacattatg
     2521 cttcctgcct ttctcgaatc tatgtatgta taatcattat cttatgacaa ttattgtaaa
     2581 cattttataa taatacttat ttttccatat ttactgtaat tatacaaaat tataaacttt
     2641 attacaacaa taaattaatt aatatagata ttagtgttta cattattgca ttatctattt
     2701 tacaattatt tacatataaa aaaatgtaat atcaaagtga agcttgatat tacatttttg
     2761 ttcttgtatt ttgtttaatt tttatgctac atcttactta aacactcatt tgcaacttct
     2821 ttatcatcaa aatgatgctt aactttacct ataatttgat aagtttcatg tcccttacct
     2881 gttataatta caacatcatc cttttttgcc atacttatag cttctgctat agcttgttct
     2941 ctatcaacaa caacatggta attttctttt ttcttgtcaa ttccttcaag tatatctttt
     3001 attattgcct ctggttcttc agttcttgga ttatcagaag ttataataca taaatcagaa
     3061 tatttttgtc caattgctcc catcaaaggt ctcttttcag tatctctatc tccaccacac
     3121 ccaaacacag aaattatttt tccttctgca aattgttgag ttgtctttag tacattttca
     3181 agtgcgtcag gagtatgagc atagtctaca attacgctta tacctttatc atttggaact
     3241 gtttcaaacc ttcctgatac tccattagaa agtctaaggc cttctttata tattggttta
     3301 ggtatgccaa gtacatagca agcagctata actgctaaag tattatatac tgtaaacatc
     3361 cctggaactg gtataaatat tacttcttca tatgaaggtg ttattaatct ataagatact
     3421 ccatccgaat ctgattttat atctctagcc ataaaatcag ccttagtatc aacaccataa
     3481 gtgtaacaag gtacatttat accttttata ttttcatata tcttttttcc accttcatca
     3541 tctatgttta ttatatttgc catagtagtc ttaaaaaata atttttcttt tgcttttcta
     3601 taatcctcta aatctttatg aaaatctaaa tggtctggtg ttaaatttgt aaatatgcct
     3661 agtttaaagt ctgtttcatc aactctattt aatgccaatg aatgtgatga aacttccatt
     3721 gcacagtagt cacatccatt atccaacata ttattaaaat gatattgtaa atctatactt
     3781 tctggagttg ttgaattgga aacaatgtct ttttctccat caaaaatttt tatagttcct
     3841 ataagtccaa ctttattctt atttaaagtt aatatctcat ttagtatagt agttatacta
     3901 gtttttccat tagtacctgt aactcctata acgttaaatt tttgagatgg atgattgtaa
     3961 aaattatcag ctacttttgc catatctttt cttgtatctt ttacctttat aaatgtacat
     4021 ccttttattg ctacatcttc ttcaacaaga aatgcacttg ctcccttttc tatggcatct
     4081 tttatatact tatgaccatc tgacacaaat ccttttatac atataaataa tgtacctttt
     4141 gtaacctttc ttgaatcata ttgtacatta ttaatatcta tatttaattc acccttgaca
     4201 ttgattatat ccaacccctg tataatatca tttaatttca taaatgcttt cctcccttta
     4261 aactctttct gctaaaatta cactacatat aatattatat taagaatttt ttactatgtg
     4321 aatggttaaa aatgcttgaa acattgcaaa aaatgctcta taattttcta taatataact
     4381 gaggaaaggt cttctataaa aactatggat aataaaatcg ggtacttaaa cgataacttt
     4441 aaaatattta atattagaga taaaaaagat atacaatttg aataccataa tcatgatttt
     4501 aataagataa ttatatttat aaatggaaat gtaacttatt ttgttgatgg tattccctat
     4561 aaactaaagc cttgggatat actctttatc agcaataatg aaatccataa accagttata
     4621 gattcaaatg tcttttatga gagaattgca atatggatta gcccagaatt tatgaaaaaa
     4681 aatagtgatg ctagtagtga cttagaaaaa tgttttaaaa tagctacaga gaataaatgc
     4741 aatagattgc gtttaaatat gcatgatatt gaatctatta agagttttat tgagcaaata
     4801 aatatttccg aatccaatac tgaatttgga aatgaaatat tgagaaatac attatttata
     4861 caacttatga tattcttaaa cagaattttc ctaaaaaatg aaaatatgga atcaacagaa
     4921 gatgtttttt atgatgaaac tattaagaaa gtattaaact atataaattc aaatatagat
     4981 gataagcttt caatagatag catatcgtct gaatttttta taagtaagta ttatcttatg
     5041 agaaaattta aatctcatac tggaacgtct atacacaatt atatagttca aaaaagatta
     5101 attttggcaa agtcactaat aaccaatggt caccttatga gtgaagtttg taataaatgt
     5161 ggattcaacg attattctag ttttgtaagg gcatttaaaa aaaactacgg tgtatctcct
     5221 aaaaattata taaacaataa aaatccttta gatattactc ttttagatga atagataatt
     5281 tatgtaaatt ttattttaat aaaacttgaa gtattagtgt atttatctta ctaatacctc
     5341 aagtttttta atgttttaaa atatatcaaa atataatgat atatatttct attacttaaa
     5401 atatatatta aataaattgc atgttaactg aacctattta tatatttatt aaatctcaca
     5461 taacttttag ttacactatc atatagctat cctatttttt caatatcatc tcttaatata
     5521 cttactctat tctcctcttt tgctgtggtt actccttttt taatgcttaa tttctgattt
     5581 tgttctaaat tcgtaaatac tacaggactt attgttgata gaccattttt ttcaatatat
     5641 tctaagtcca ttttaactaa tttttctccc gcttttactt catctcctac attcacaaat
     5701 acttcaaatc catttccttc taagtttaca gtttctatcc ctaaatgtat aagtatttct
     5761 actccacttt tttgggattt tattattatt gcatgcttag ttggaaatat aactccaata
     5821 gttccatcaa ctggtgaata aatcaatcca tcactagatt taattgcaaa tccatccccc
     5881 atcagtttac ttgaaaatac agaatctggc acttcttcta tatctaataa ctctccattt
     5941 gttaatgaat caatcttttc tactaaagta ttttccatat ttgcaacttc catatttttt
     6001 ttcactttat ttttatcaac atttttagta acagtacttt caacctttgt ttttccctct
     6061 attatatctt ttatatcaga ctttattgaa tctgattttg gtccaaatat agcttgaaca
     6121 ttgttgccaa taaccataac acctgctgct ccaagtgatt tcaatttttc tttatctact
     6181 ttatttatat catttacaac caatctaagt cttgttatac acgcatctag actctttaag
     6241 ttgtctttat ttcctaaagc aatcaaaatc tctgatgcta attcattctt agacatttca
     6301 atgacttcag cgttagtttc tgactcatct tctcttcctg gtgtctttaa attaaatttt
     6361 cttatcataa atctaaatac actatagtat aatactgcaa aaccaatacc taccaagata
     6421 gccaagtacc atggagttct attaggaaga acaccaaata ataagaaatc tattaatcct
     6481 cctgaaaatg tgaatccaat tttaactcct aataattgca taattgcgta tgataaacct
     6541 gaaaataaac aatgtattgc aaataatact ggtgcaacaa ataaaaacgc aaactctatt
     6601 ggctcggtta ttcctgttaa aaaagaggtt aatgctgctg atgccattat acctgctaca
     6661 aattttttct tttctggctt tgcttcatgc accatagcaa gagctgctgc tggaagacca
     6721 aacatcataa atggaaatgc acctgtcata aaagtacctg ctgtgaaatt tactccatct
     6781 ttaagttgag caaagaatat cttttggtca cccattacaa gctcaccagc tttatttaca
     6841 tattctccaa attgatacca aaatggatta tggaaaacat gatgcaaacc aaatggtatt
     6901 aatagtcttt ccaaagaacc ttccataaat gctgctagtg tttgatttgc ttctataaca
     6961 ttttttgaga aactaagtaa tccctcttgt attggaggcc aaataaaaga caatataatt
     7021 cctattgcta tagcaactaa tgaagtaacg attggtacaa atcttttacc tgcaaaaaaa
     7081 cctagatact gaggtaattc aattttataa aactttttat aaagataagc agccactatc
     7141 cctataataa taccaccaaa tacccctgtt tgaattgttg gtattccaac aatatttgca
     7201 tacatagaat tgtctaccat atcttctgta acacctgcca taacacccat agatgtattg
     7261 agaattaaaa aacctactat ggctgccaat gctgcaacac cttctccttc agttaaacca
     7321 acagctaccc caactgcaaa tataagtggt aagttattaa atataatttg acctgaactt
     7381 tccattatac ttattataag ttgaaatgta ggattcgcca aagctggaaa atgagccaac
     7441 aatgcgtctc ctgctaaagc attactcacg ccaagtaata tacctgctgc aggaagtaat
     7501 gcaactggta gcattagaga ttttcctact ttttgtaaaa caccaaatac ttttttcatt
     7561 tacttttctc tccttattat tttatatttt tttaaataaa aaagacttaa gtttataaaa
     7621 agttacatta gtatacttaa ttctaaaatt aaacttatgt taatataaac aaacctatat
     7681 taacctaatt tcagaattaa aaataaacta ttataatttt tttataaaca taagtcttgc
     7741 ctgcattatc agtaacaatc ttaatgtttt aaaattatta ttttatatta aattttaaat
     7801 aatatattaa aattaaacat aatatattaa atctatacat tctgtattaa actactcttt
     7861 attataaatt cagtacagat ataactcttt tgatatgtaa tgttaaataa agtagttcat
     7921 cttgacttac atagtaatca taattctttt ctacatgagt ttttatttta tatgcacaat
     7981 cataatcctt ttcatacttt actttaatta tatctataat ttcattattt gtatcattga
     8041 ttttttcttt ttttactaat cgttttgcaa agaattttaa atgagtcaga agcctatcat
     8101 aatttatatc atcttcttta aattcaactc tataataata tcttataata tttagtatgt
     8161 cttttacaat tttagtaatt aacagtgatt cctttgaact tcccttatag tttgaattta
     8221 ctatatgcat tgctataaaa ccagcttcat ctactggaaa tttcacatca aattctttat
     8281 ttatataatc tacagcccat ttcccaatct catattcttt tttatgtatt ctttttatct
     8341 catgtagtaa atcattcttt atagttatgt tttcatgata tcttttaatt gcaaatgcaa
     8401 tatggtcagt aagtgctaca taaatatatt cataaagttt agtattcaaa ttctcctctg
     8461 cataagcaat tatttcagta ctaacctcaa aaaatttctc atcaacctgg cttgctagtt
     8521 tactaatttt atctaaaaag ctttcatctt gtattacaaa agttctttca attttacttt
     8581 tatctatttc ttgacctttc tttttttgaa acccaattcc acatcctgtt aatattaatt
     8641 ctttttttat attttcatct aaaactaata caacattatt attataaatt tgttgaataa
     8701 tcataatcaa tatctctccc aaaatacaat ttcccttatt cataaaaaaa tacctaaatt
     8761 aagatacaat tcacctgtat accttaacct aggtattgcc tgcttaccag taacaatcca
     8821 gcttgttaac gcttatatat tgtattcaat ttatggtttt attctattat aaataaatat
     8881 atatgtcaag taaattattg aaaactattt cctatttttt agtattaatt ttaacatcaa
     8941 aaatcaaatt ttattccatt atatttatga ttctatcatc atcatttcat catagagaat
     9001 attttccaat aactctttag ccacttctct tgatatttgc ttagtttcaa ctaattgttg
     9061 aacactgtct ctttgacttt gtatcgccat tactctgtag tattctactc ttgcctcaaa
     9121 cttatcatca ttttgaatct ttctagtaat ccaattcaaa taacgattat agaatctgat
     9181 tgctattaag gcctcatgtc tgttatcttc atcagtattt ttatttataa atgatatagc
     9241 agttttagta gaaagtgcat atgcatggtg aaactctttg attatttctt tattctcaga
     9301 tactattttt gtaatttttt cttttacttc tttctttact tttctattta taatccacat
     9361 gcttattttt aattttacaa ttttaaatat agatttttga acagattttc ttgattttgt
     9421 aatataaaat ctatacaatt taaaggctct atcagaaatt ttaccatctt ctaatagttt
     9481 caaaacagct aataatccct gttggtctgc ttctgcaatc atcatactta taagtttatt
     9541 atctggagta tttagtattc ctttttctga gtctaccaac tgttcattta aatcatgtat
     9601 aactggttgt acttcttgtg ctttttcatt actaagtttt aaaatggctt ctttcaaaat
     9661 tagtttatat gcctctttat cagataataa aggttctgac tcttgtacgt tattttttaa
     9721 taatcttggc agtaaaactg ttgccaatac taaacttata agaataactt ctgatgatat
     9781 aaataaaaca gtatctctca ttggaaatac atctttatca gccaaataaa aaggtataga
     9841 taaggcagtt gctagtgtaa atgtaccatg aacaccacaa gtagcaacta ttagtgagta
     9901 tttacattta gaaatactct cttttacaac ttcttcatcc ttaaaaccta aaaaacctac
     9961 aaaaaaatta tttaaaggat tctttttagg ttttataaaa gaattatgcc ataaatatac
    10021 ccatataaat cttataacaa ataaaataag tacaattaaa gtagaaataa aagtcaattc
    10081 caatgccata ttttcatttt tactactaag tcctgtatat atacttggta acaaaaaacc
    10141 aagcagtgta aagacaaatc cattcaatac atactccaaa acataccaag tattgtctga
    10201 tattatcctt aatttagtag ttgtattttg taaatgctct ttttcaattc catgagctat
    10261 ccctgccatt acaacagcta ggattccaga tactcctatt tcttctgcta caaaatatac
    10321 aaaaagtgga gtcattattt gtataactat taccattgga atttcttcca aattccactt
    10381 gtgtaaacta agtcttattt ttataattat gtatcctaaa atacttccta ctattattcc
    10441 tccaaaagct gtaatcataa attgaatccc tgcctcttct attgaaaaaa ctcctgtaac
    10501 tgtggcaagt actgcaacct taaatgctac tactccagca gcatcattta ataaagattc
    10561 accttcaagt atactcatca tatttttagg aaaatttaat ccttgtgtaa ttgattttac
    10621 tgctactaaa tcagttggag atattactgc tgccaaagca aatgaaacag ataatggcat
    10681 cccaggaata agaaaatgta taaagattcc acctgcaaac acggttatta aaactaaacc
    10741 aaatgcaaga agcaaaattg gttttctgag ctctaatagc tcttttctcg atacattttg
    10801 tccttcactg aatagtaatg gagctatgat acatattaca aatatttctg gattaaatga
    10861 aaatgagaat ttaaatggca aaatagacaa tataatccct agtacaattt gatatattgc
    10921 aagaggaata ttactccact ttttgctaaa tacagttgaa atcgttgtac aaaccaataa
    10981 tcccaacaat gttactaaaa ttgacataac tacctctctt ttcaataata atattataga
    11041 ttaaacttat atatcaacta acttaatatt aacagcatga aatcccatct taatcaaaaa
    11101 aattttcagt ttattctatt attcttatta tatacatcaa atttaatttt tgaaatatct
    11161 atactagatg acatgcaaca aaatgatttc ttgatatttc ttttaaaact ggaatctctt
    11221 ttttgcatat ttccttacaa taagggcatc ttgtatgaaa cttacatcca cttggtggat
    11281 ttgttggaga tggtatgtct ccttttaata taattctatt tttcttaatt gtagggtctt
    11341 gtattggtac tgcgctcata agcgcttttg tatatggatg ttgagaatgt aagtaaagtt
    11401 ctcctttagt tgcaatttct attatagaac ctagatacat aacacaaact ctatgtgcaa
    11461 tatgatttac tacactcaaa tcatgagata taaataaata tgaaagttta tatttatcct
    11521 gtaaatccat aagtaaattt ataatttgag attgaataga aacatctagt gcagaaacag
    11581 gttcatcaca aactataaat tctggattta atgctaatgc tcttgctata cctattctct
    11641 gtctttgacc tccagaaaac tcacctggtt ttctatttaa atggtactta gaaagaccac
    11701 aactttctac aacatttaat atattatctt ttatttcatt ctttggcact aaattatgtt
    11761 ctataactgc ctcggatatt atttgttcca catttaatct tggatttaat gaactatatg
    11821 ggtcttgaaa tatcatttgc attttgggtc ttaactttct aagttcttcc ttctctaact
    11881 tatatatatc tacaccatta aattcaactt taccttcagt tttttcaatt aatctaagaa
    11941 tggttcttcc tgcagttgat tttccacatc ccgattctcc aactatagcc aaagtttctc
    12001 ctttttttaa atcaaaagaa attccatcta ctgcctttac acatttaatt tccttatcaa
    12061 ataatccaga tttaacttca aagtactttt taagaccttc tacttttaat aaactctctt
    12121 cttttttcaa ttatatctcc tcctttttat ataaccaaca tgcaatacta tgaccactat
    12181 tcaattctat taaaggaggt atctgagttc tacatttatc acatactttt tcacatctat
    12241 cactaaaata acatgaatct ggcatcccaa ttggatttgg tacttgtcct ggaatcgaat
    12301 acaatctttt atctttgttt tttccaagag caggctttga ttttagaagt ccaatagtat
    12361 aaggatgttt tggattttta aataattcaa caacctctgc cacttctata attttgccag
    12421 catacatcac taatactttg tcacacattt ctgcaactac tcccaaatca tgtgtaatca
    12481 tcattataga tgtgtttaat ttttctttta tatttttcat tatatctaaa atctgagctt
    12541 gaatagtaac atctaaagct gttgttggtt catcagctat aagtaatttt ggattacatg
    12601 ataaagccat agctatcatt attcgttgtc tcattccacc tgataattca tgtggatatg
    12661 aattatatat ttcttcagct cttggtattt ctaccagttt tatcatatcg attacctttt
    12721 tcttagcttc ttcctttgat aaatcttgat gtatcataat accttcaatg atttgacttc
    12781 caatagtaaa cgctgggttt aaagatgtca ttggttcttg aaaaatcatt gatatctcat
    12841 tccctcttat tcccataagt tcatcttcat tcatctttaa aatatctttg cctcgaaaga
    12901 ttatttctcc accttctatt tttgatgttg gaagtaactt catcaaagac atggctgtta
    12961 cagacttacc acatcctgat tctccaacta ttccaatagt ttcaccttca tttatattaa
    13021 aacttacttc atttacactc tttacaatcc ctttatctgt atgaaaataa acttttaaat
    13081 ctttaacctc aattagtgct ttactcatta ttttctcctc tcagcaaaat tacttaagat
    13141 aacatacacc ttttgattgt attttataaa aatttaaatt acaacatttt cttacatttt
    13201 attatttttt tctattaatt ataataatat aatagttaat ttctatatat attgatttaa
    13261 taatgattac tattatacaa taaaaaatat gtgttaaata ttattttatg gaggttttat
    13321 aagaatatga aatttaaaaa attggcatca ttaatcttag tttcatcatt aatgcttaca
    13381 tttacagcct gtgcttccaa tgataaagac aagtctagtg gaagcaaatc tggtggaact
    13441 gttgtaatac ctatggcaag tgacccagat ataatcaatg gagcttttgc aaacgttaaa
    13501 gaagactctc ttgcatcaaa tatagtttat gctcctcttt atacttatga aaaaggaaat
    13561 ttagtaaatt atttagcaga aaaagtagat tttaaagata gtaaggaatt aactataaaa
    13621 ttaaaatcca atttaaaatg gcatgatgga aagccaataa ctgctgaaga tgtcttattt
    13681 acatttaata cagttttaga cgaaaaacaa aattcaccat caagacaata cttattggtg
    13741 ggtgagaaac cagttaaagt agaaaaaatt gatgatttaa cagttaagat tactttacca
    13801 actgcaagtg aatctttctt atatggtata tctaaaattt ctccaatacc aaaacatgtg
    13861 tttgaaggag aatcaaatat agctaagtca gaaaaaaata ataatccagt tggttctggt
    13921 gcattcaagt ttaaggaatg gaaaaaaggt gaaagcatag tatttgaaaa gaatgcagat
    13981 tattttggtg gagaatctaa ggcagattct atagcgctaa aaataattcc taatgaagca
    14041 tctcaagaag cagctttaaa taatggcgaa atttcactta tgaagacatc tgctgaagga
    14101 tatgaaaagg caaaatcaaa ctcaaattta caaacttata cttatagtga agaaagatta
    14161 aactacattg tatttaacca aaatatatca aacatggcta acaaagaagt aagacaagcc
    14221 ttatcatatg ccttaaatag aaatgaaatg atagaatcag catatggaaa agaaggttct
    14281 gtacctgcta aatctatatt agttccagaa gcagacttct atacagaaga aggtgttgaa
    14341 ggatatgacc aagatacaaa taaggctaaa gacttattgg ataaaagtgg tgttaaaatt
    14401 gataaattaa aaataggtta taacacaggt agatttgggc ataaaaacta tgccttagta
    14461 gctcaacaag agttgaaaaa aattggtata gaagctgaaa tagttcctta tgaaagtaag
    14521 gcattcttca atatactatt tagtaatagc actgaatgtg atatgtatgt aaatggttat
    14581 gcttggggat tagaaccaaa cccatataga ggtatgtttg aaactggtca atattgcaac
    14641 caaactaaat attctaatgc tgaaatagat gctttatggg aaaaaggatt tactgagtta
    14701 aacaaggaaa aacgtgaaga aatatataag caaatacaac aggatatatc taaggatgcg
    14761 cctatttaca ctatagatta tgaacaaaat ttaatggcag ctcaaaaaaa tctaaaagga
    14821 ataaaagatg ctaaaccatc tcctgcaata ttatttgaag attggtctaa actgtatgta
    14881 gaataattga tgtaaaattt tatattatta attagaaaag gagatgtcta aaaataatat
    14941 tgttttatta ttttaaaacg agacatctct tttaattagg agaatttact tatgttaaaa
    15001 ttaatattga atagagtaaa agttttaata cctatgttaa tcttaatatc tatattatca
    15061 tttggacttt tagaattagc tcctggtgac ccagcagatg catatataaa tcctttaatg
    15121 tcttcacagg atattgaaaa tataagggtt aatatggggc ttgataaacc agcatatata
    15181 agatatttaa actggttgaa aaacacttta agtggaaact taggtatttc ttatataaac
    15241 catatgcccg ttacagaaca aattatggaa aaaatgggaa atacatttat tcttatgggt
    15301 acttctttga tattttcaat attggtagcc atacctcttg gaatattttt agctgtaaat
    15361 aaaaattcaa taactagtaa agtatcatct gtttttaatt atataggtgt ttctatacct
    15421 tctttttgga taggtatgat actcatttca attttttcag ttaaacttaa cattttccca
    15481 tcaggaggta tgcatactat tggaaatgat agtattggag atttagcaaa acatttagtt
    15541 ttgcctgtaa taactttagg tctatataat actgctattt ttacaaatta tgtagaagct
    15601 agtgtaaatg aacaattaaa aaaacaatat gttattacag caagagcaaa aggattatca
    15661 gaaaaagtca tactatttaa acatgtcttg aaaaattcac tgacatcatt ggttacaatc
    15721 ctaggcatga gtatacaaaa attagtcact ggtgcatttg ttacagaagt agttttctct
    15781 tggccaggta tgggaagatt aatgattgat tcaatttttt caagagatta tactgtaatt
    15841 atggcaataa ctatgttatc agcactattt ttaattttag gtaatttagt tgctgatata
    15901 ctatacttat taattgaccc taaaataaaa tctagcaaag gaggattcta gttgtgttta
    15961 caagtatctt aaaaaaatta aaaaaagata aatcctttgc ttttgcatgt attactctta
    16021 gcataataat catattaatt atagcttctt ttatattaaa tatagactct aatgcaattg
    16081 atactacttc taaacttcaa gaacctagct taaagcatat ctttggtaca gacgaacttg
    16141 gtagagatta cttctctaga gctctttatg gtggaaggat ttcacttagt gttggtattt
    16201 tatcaatgct tatttctgta gtatttggta ctgctgtagg tgttattagt ggatatattg
    16261 gaggtaagct agaccaattc ttgatgagat tagtggatat gcttatgagt ataccaagtt
    16321 tcttagtttt gattgtatta aatacttatc taacacctaa agtttccact attatactaa
    16381 ttattggatt cttaagttgg atggaagttt caagaattgt acgtggcgaa acattaaaaa
    16441 taaaggaaaa tgaatactgt ctagctgcta aagctactgg tatcaaaact agaaatatac
    16501 tattaagaca tatagtttta aacttaaaag agactattgt agttgctggt tcgttaaata
    16561 ttgctaatgc tatcttaact gaatctgctc ttagtttttt aggtcttgga atccaattgc
    16621 ctcttgcatc atggggtaat atgcttcaag atgctcaaag acatattttt gacaagttat
    16681 atttagctgt atttccagga ttacttatat ttttaacagt catgagtttg actataataa
    16741 gcaaaaaatt ttctagtaat acttaacaaa ttttatatag aaaataaaaa cagtgattat
    16801 ctaaagatat acactgtttt tatttttcgt ttaaaaaaat acatattttg tatattataa
    16861 ctttaaatac tactctacca tgttatgcaa agcgtattat tgctctcaaa catatagatg
    16921 aaaaactaat agtttattat aataaaaatt gatttattta tttgttattt taataacatt
    16981 tttaagagtt ctttttacta aagaaatcta taataaattt aaatacatgt tggtctacat
    17041 ggctataacg ttctttttta ggtctacata atataacttt ccatgtaata ggatgattaa
    17101 aaggcttttc tataattttg ctattttcaa aaaaatcatg aactggtgat ggtaaaattg
    17161 ttataaaatc agaatttgtg gttgatagca acaaaaaatc ccatgaacca gacattatat
    17221 atctttttaa cttaacattt tcatcagtaa atttttgcat tagcttatga tgtatcataa
    17281 aagtaggatt aaatattgca agtaactgct cattcaaatc gttccaatct atcttatcct
    17341 tcttagcaag tggatgattt atatttaaaa atgctgtaag ttcatcttct tgaagcacat
    17401 gttcagttac tacactttta tcaattttat gtggatgtaa caatactgca taatctaatt
    17461 cctgtaagat aagcattcta tttaagtcat atgcaccctt ctctactata tcaaattcta
    17521 tatctggatt atttgcaact aactgtgcaa ctacatctga aaatgctatt ccaagtataa
    17581 gtggaggtat tcctattcta acttttcctt taaattgaac tgaatcttct cttaaatctt
    17641 ccatcatatt tttgtattca tttatgatat gttctgcatt tacataaaat ctttctcctg
    17701 ctggtgttaa cccatttaac ctgcctttat acctttcaaa taaatataca tcttcacttt
    17761 cttcaaaatt ttttataatt tgacttaatg ctggttgaga tatgtgaagt gtcttagatg
    17821 ctattgataa attaaaacca gatttcacta tttcaacaaa atatttcaac tgttttatat
    17881 ccattttaaa cccccttccc caaaagtata atttagatta tattttttct tctaatgata
    17941 ttttatagga aaatcaacat aatttcatta atattttaac aaaatatttt tctaaatcta
    18001 ctataaattt ttatcctata aatttttctt atacactata taaaaaatta gacttagaca
    18061 aaacgctttt ttcgccgtaa tctataatta gaaacaggaa aacctaaagc attaggaata
    18121 aaagtaattg aaatacttaa aaacaagctt gtattaaaaa agtactttat tatagtgttc
    18181 cctaatttct ttattcagaa aatttttaat tatcattcaa gaaaggtaac tattagatat
    18241 gaaagatgta gtaatcgtat cagcagtcag aacacctata ggaagttttg gaggtgtatt
    18301 taaaaatact tcagctgttc aacttggaac tatcgctgtt aaagaagcga tatcacgtgt
    18361 aagacttaat ttatctgaaa tagatgaagt aataattgga aatgttcttc aaacagggct
    18421 tggacagaat gttgctagac aaattgctat aaatgcaggt ataccaaatt ctgttccaag
    18481 ctacacagta aataaattat gtggttcagg gcttaaaagt gtccagctag ctgcccaatc
    18541 aatcacatct ggtgaaaatg atgtggtaat tgcaggtggt actgaaaata tgagccaagc
    18601 tccttatatc gttccaactg ctcgctttgg tagtaaaatg ggtgacataa ccatggtaga
    18661 tagtatgcta acagatggcc ttattgatgc tttcaatcag tatcatatgg gaataactgc
    18721 agaaaatatt gcaactaaat ttgaatttac tagagaaatg caagataaat tggcattaga
    18781 aagtcaaaat aaggctgaga atgctataaa aaacaaccgc tttaaagaag aaatcgttcc
    18841 agtagatgtt ttaatacgtc gaggaaaaat agaaactatt gataaagatg aatatcctaa
    18901 attaggtatg acatttgaag gtttatctaa acttaaacct gcattcaaaa aagatggtac
    18961 tgtaacagct ggaaatgcct caggtattaa tgatggtgcg gctatgttaa tactaatgag
    19021 ccaacaaaaa gcagacgaac taggtattag accacttgct aaaataaaat cctatgcttc
    19081 agctggtgtt gaacctgaag tcatgggtac tggtccaatc cctgcaacta gaaaagcact
    19141 gaaaaaagct ggacttagca tcaatgatat agacttaata gaagcaaatg aagcgtttgc
    19201 tgcccaagca ttagctgtta aaaatgaact tcaaatagat tcatcaaagt taaatgttaa
    19261 tggtggtgca atagcattag gacatccaat aggagcaagt ggagctagaa tacttgttac
    19321 cttaatttat gaaatgcaaa aacgtaaagt tgaaactgga cttgcaacac tttgtattgg
    19381 tggtggtcaa ggaatatcaa tggtggtttc acgataggag gaaatatgaa taaaatagtt
    19441 agcattgatg aagctctatc tcatgtaaaa gatggagata caataatggt tggtggattc
    19501 atggccaatg gttcaccaga aaatttaatt gattatttat gtagtaaaaa tataaaagat
    19561 ttgacactaa tttgcaatga tactggcttt attgacaaag gagttggcaa aatggttgta
    19621 aataagcaat ttaaaaagat aattgcttct catgtaggat taaataaaga aactggaaga
    19681 caaatgaata acaatgaaac agatgttgag ttagttcctc agggaactct tgctgaacaa
    19741 atacgtgcag ctggttatgg tctaggaggt atactcacac cgactggtat tggtactctt
    19801 gttgaggagg gaaagcaaaa aattgttgtc gatagcaaag aataccttct tgaaaaacca
    19861 atttatgcag atgttgctct tctatttgca tcaaaggtcg ataaagcagg taatctagtt
    19921 tataaaggct ctatgaacaa ttttaataat ttaatggcat ctgctgccaa aattacaata
    19981 gttgaagcag atgaaatcgt tgaaattggt gatattgacc caaatgaagt taatacacca
    20041 ggtatttttg tgaattacat cgttgaagga ggtaatttat aatggataaa ttagaaatgc
    20101 aagaatatat tgcaaataga gtatctaaag aattaaaaga cggagccgtt gtaaaccttg
    20161 gaataggtct accaaccaaa gtagcaaatt atattccgga taatgtaaac gttatcttac
    20221 aatctgaaaa tggcttttta ggtttaggtt cagccgaaga tggaaattca agtgatgaaa
    20281 ctattgtaaa tgcaggtggt caaccagtta ctatacttcc tggaggatgt ttctttgata
    20341 gtgctacttc ttttggaata atcagaggag gtcatgttga tataactgtt cttggcgcac
    20401 ttcaggttga caaatatggc aatatagcaa actatatgat tccaggtaag atggttccag
    20461 gtatgggtgg agctatggac ctagtaactg gagctaaaaa agttattgta gcaatggaac
    20521 atacttcaaa aggttctgca aaaatattaa ataattgtac tcttccactt actgcaacaa
    20581 atgctgtaga tttgatagtt actgaaatgg gagttatgga agtaacatca gatggaattt
    20641 tacttaaaga aataaatcca gctttcacat tagatgatgt tatttctgct actgaagctc
    20701 ctttaatatt gtcagattca ttaaatggaa aaatatctgt tgtttaaaat aattaaaact
    20761 agaaaataaa aatctcttag acgtattaca tataaatttg tattgcttaa aatattaata
    20821 taaaaataaa ataaaattgg aggaaatgaa aatggttaaa gataaagttg tattcgtaac
    20881 aggagctgca agtggaatag gtaaacaaat agctgaatcg ttcttaaaaa atggtgctaa
    20941 agtaatgttc tctgatataa atcaagaagc tttaaataat accataacag agttacaaaa
    21001 aaaaggttat gattgcatga gtgtaaagtg tgatgtaact aaggaagaag aaattaacca
    21061 cgctatagac aaaactgttg aaaagtatgg cagattagat atattaataa acaatgctgg
    21121 acttcaacat gtttctatga tagaagattt cccaactgat aaatttgaat ttatgataaa
    21181 aataatgctg acagcacctt ttatagctac taaaaaagca tttcctataa tgaaaaaaca
    21241 aggctttggt cgtataataa atatggcctc aatcaatggt gtaataggat ttgctggaaa
    21301 ggctgcttat aactcagcaa aacatggact tattggacta acaaaagttg ctgctcttga
    21361 agctgccgat tctggtataa cagttaatgc tttatgccct ggatatgtag atactccact
    21421 tgtcagaggt cagttcaatg acctttcaaa aactcgtaat ataccattgg aaaatgtctt
    21481 agaggaagtt ttatacccac ttgtaccaca aaaacgccta ttagatgttc aagaaattgc
    21541 cgactatgct atgttcttag caagtgataa tgctaagggt attacaggac aagcttgctt
    21601 actagatgga ggatatactg cacaatagtt attatattta gggcttgaag aaagtattct
    21661 tcaggtcctt ctactaagga gggatattta tgagtattat cgcgccaatt ggtataatta
    21721 ttggtatcat agctattata tatttttcag ttaaagaaat tcatataact atagctgctc
    21781 caatagcaac tttaatagtt gttattttaa ataaaatgga tgttgtaacg tcaatgctag
    21841 gtgctgacaa aaacaattat atgggtgcac ttggaaacta tataatgagt tattttgcaa
    21901 tattcttatt aggctcaata cttgctaaat ttatggaaga aagtggagct actgtatcaa
    21961 tagctgattt tattttaaat aaatttggct ctaatcatcc ttatagagtt ttagttgcta
    22021 tatttatagt aagttttata ttaacgtatg gtggtataag cttatttgta gttatgtttg
    22081 cggtcattcc acttgcaaga actctattta aaaaacttga tatttcttgg aaccttattc
    22141 agataccact ttggcttggt attgcaacaa ttactatgac tataattcct ggaacaccag
    22201 ccattcaaaa tgttatacct attcaatatc taaatacgtc tcttactgca gctactatac
    22261 caagtattgt aggtagtata ggttgtgcta catttggact tgtctatatg aaatatgctc
    22321 taaacaaaag tattagaaaa ggtgaaactt atgcaactta cacttcagaa ttagaagaag
    22381 aatctttaga tagagaatta ccaggatttt ttgcaagtat ttttcctcta ttagcactcg
    22441 taattatagc tataactgga agtacttttg gttcagaatt tatgaaaaaa aatgtaattt
    22501 acatagcttt aatagttgca attcttttat ctgctttttt attccataaa tttctatcat
    22561 ctaagataca gacattgagc ataggtgcta gtggttctat aggtcctata tttgctacat
    22621 cttctgcagt tgcatttgga gcagtaataa tggctgcacc tggttttgat gtatttagca
    22681 aacttataat gtcaatacca ggtagtccac ttataagtct tactgttttg acttcaacaa
    22741 tgtcagcaat aacagggtcc tcttctggtg cattaggaat agtaatgcca aactttgcag
    22801 attattattt aagtactggt ctaaatccag aattaattca ccgtgttgct acaatagctt
    22861 caaatatact aactatagtt ccacaaagtg gtgtttttct tacattctta agcttatcta
    22921 agttaacaca taaaactgga tttaaagaaa ctttcattgt agttgcagtt ggttcactaa
    22981 tagcagaaat tattgtaatt acattaggat tactaatgta ttaatataaa acaagttaaa
    23041 attcataata tgaaaggtgg tgtctcaaag tgaacttttt acttaatgag acactctttt
    23101 tatatgaaat aacatatgcc tatcatttaa acaataaaaa aggccttatc tctattttga
    23161 gacagaccct ttttatttct taaagattta aataattata atttgttgct aatacgaaag
    23221 catctactga ataaatttaa ccaaataaaa aaaaatttat cataactata aatttacgtt
    23281 atggaattat tataaaaata ttattttctc taactaagta attccatact atacaggctt
    23341 ttaatacaaa ttgtcaaaca cactgaaata tacctattta ttccatatat atctttttta
    23401 catatcttta cagtaaaaaa agtcttaatc ttatgttagt atagattcac gggtaacccc
    23461 ctacactaaa ggttacaaat cagtaacatt tttagaattt ttagattaag aggtgtttaa
    23521 tgatgagaag aaatatacca acaaaattaa aaactatatt agctttagga gtgttaactt
    23581 tagcaacagc ttcaagtgct gttactgcaa gtgcttcatc acttgaaaat aatgtaaata
    23641 taagtaacaa aagtttagta tatagtaata ctaaatacaa gttttctgca aaaccaaatt
    23701 gttctgtaaa accagaactt aagcctgata aagataactc ttgtaaggat aagaatcata
    23761 atgacaagaa ttgcaacaac actaataaac ctgaggacaa caataattca gactctacta
    23821 ataagcctga taacaataaa cctgagtcta ataaacctga agacaacaat aattcaggct
    23881 ctactaataa acctaataat aataaacctg agtctaataa acctgaagac aacaataatt
    23941 caggctctac tgataaacct gataacaata aacctgagtc taataaacct gaagacaaca
    24001 ataacaataa ttcagactct actaacaaac ctgatgataa taacaactca ggctctacaa
    24061 gtgaaagttt ttcagcttac caaaaagaag ttgttgactt agtaaatata gaaagagcta
    24121 aagctggatt aaatcctctt actcttgatt cttctatatc aaatgttgct acaaaaaaat
    24181 ctcaagatat gattgataac aactattttt cacacaattc tccaacttat ggttctccat
    24241 ttgacatgtt gaaaaaattt ggaataagct ataagacagc aggtgaaaat atagctatgg
    24301 gtcaaaaaac tcctaaagaa gttgtaaacg catggatgaa ttctgaaggt cacagaaaaa
    24361 atataatgaa tccaaatttc agtaaaatag gtgttggagt agcacaaaaa agtggtggtt
    24421 caatttattg gacacaaatc tttgtaggat aaaatactct ttttccatac atatcaaaag
    24481 agtatctcta agtgataaaa aacacaaatg agatactctt ttgtatctat tttaaaatta
    24541 tcaaatccaa atatacatac tataaatatt atttgttctc ttaattttag tgtgtagaac
    24601 aatatataaa aagtattggc aagatgaaaa tggaaaactt aaaaaagata tttaataggt
    24661 aaaaacaaaa tcttaatatc ttcataaaaa aaggctgtct caaaatagag ataaggcctt
    24721 tttcattgtt gaaatgatta aaatatattt gatttcatac aaaaagagtg cctcatgaac
    24781 taaaaagctc actttgagac actctctttt atcaatttat ataagcatta taatacttta
    24841 atattattca gctaattttt tgtatccttc atatctttct ttagcatcag cttcagcttt
    24901 agcaaataat ccatcagctt cttctgggaa ttgtttagct atagcagaat atcttacttg
    24961 tgcaagtatg aaatctttga agcttgcagt tggttctttt gaatctaaag tgaatgggtt
    25021 tttaccttca gcttttactt ctggattgaa tctgtataaa tgccagtatc cgcaagcaac
    25081 agcttgtgct tcgttagcta cagttcttcc cataccttct tttaatccat ggtttataca
    25141 tggagcataa gctattatta gagatggccc atcatgtttt tcagcttcta taacggcttt
    25201 gataaattgg tttttatcag cacccatagc aacttgagct acatatacgt ttccatatgt
    25261 catagccatc attcctaaat ctttctttct agatttttta cctgaagcag caaacttagc
    25321 catagcagca gctggagtag ctttagaagc ttgacctcct gtatttgaat atacttctgt
    25381 atcaaataca agtacgttta catcttcacc agatgcaaga acatggtcta atccaccgta
    25441 accgatatca tatgcccaac cgtctccacc tagtatccat tgagatctct taactagata
    25501 atcttttcta tcttttatag cttttactaa ctctttagct ttttcatctg tacaatcaca
    25561 agctggtttt tctaataatt caacaacctt agcacttgct actttagaag cttctccatc
    25621 atttctagaa tctaaccact ctgtaaatac tgctttagca tcttcacaga tacccataga
    25681 aactaactct gtcatagcat ctactatttt atttcttatt tgcttaacag ctgtgtacat
    25741 accaaatcca tattcagcat tatcctcgaa taaagagttt gcccaagatg gacctttacc
    25801 ttcatggtta caagtataag gaattgatgg tgcagaagct ccccatatag aagaacatcc
    25861 agtagcatta gctatcatca ttctatctcc atataattgt gtaactactt taatgtatgc
    25921 agtttctcca catccagcac atgctcctga aaactccatt aatggttgtc taaattgact
    25981 tcctttaaca gtgtttacag gcataacatc accttttgga gctacctcat caggatttac
    26041 tgcaaatgcc caattatcta actctgtatc aagttgagta tctattggct tcataactaa
    26101 agctttttct ttagctggac atatgtcagc acagtttcca catcctgtac agtcaagtgg
    26161 actaagttgc attctatatt ttaatccttc taatccttta cctaatgctt ttttagcttc
    26221 aaatccttct ggagcattag ctaattcttc ttcagtaact aatactggtc ttatacatga
    26281 atgaggacat ataaaagaac attggttaca ttgaatacag ttatctatta tccattcagg
    26341 aacatcaaca gctataccac gtttttcata agctgcagta ccacaaggga atgttccatc
    26401 ttctatacca ttgaatgtac ttactggtaa gttgtttcct tcttgtgcag tcattggtct
    26461 tagtatattt tttataaatt ctggttcttt aacttctcct ttatctgcat caacagcatt
    26521 ttcccaagaa gctggaactt caactttaac taaagcattt attcctgcat caacagcagc
    26581 atagttcatg ttaactattt tttcaccttt tttaccataa gctttaacta tagaatcttt
    26641 taagtattta acagcatctt cttctggtat tatttcagct aatttaaaga atgcagattg
    26701 cattatcata tttattctgt ttccaagacc tatttcttgt cctagtttaa cagcgttaac
    26761 tgtatagaat tttatatcat ttttagctat atattgcttc atatgagctg gtaaatgttc
    26821 ttcaagtcct gcttggtccc atatagtgtt aagtacaaat gtaccacctt ttttaagacc
    26881 ttttaataaa tcatattgat aaacatatga ttgtttatga caggcaatat aatcagcttc
    26941 atctattaaa taagtagctc ttattggagt atcaccaaat cttaagtgag acatagttat
    27001 accaccagat tttttagagt cataatcaaa gtaagcttga gcatattttt cagtatggtc
    27061 accaattatt ttgatagctt gtttattagc tccaacagta ccgtcagaac ctaatcccca
    27121 gaacttacat cttatagttc ctttagaagc tatttttatt ggctcagatg gagttaaaga
    27181 agtattagtt acatcatcaa ctatacctac tgtaaatcca tttttaggtt gttctgaaac
    27241 taagttatca aaaacagttt ttatatctga aggagtagta tcttttgaac ctaatccgta
    27301 tcttccacct attatcatag gagcattttc ttttccatag aatacatcac gtacatctaa
    27361 gtataatggt tcaccagttg aacctggctc tttagttcta tcaagtacac atattctttc
    27421 aacagtagat ggcatagcat ctaagaaatg tttcattgag aatggtctat ataaatgaac
    27481 tttaactaaa ccatattttt ctcccttagc atttaagtaa tctatagttt cttctattgt
    27541 ttctgttact gaacccatag ctattaatac gtatttagca tcttctgctc catagtaatc
    27601 aaataaacta tgttttctac ctgttaaatt actcattttt tccatgtatt tttcaactat
    27661 acctactata ttttgatagt atttattaga agcttctcta gtttggaaat atatatctgg
    27721 gttttgagca gtaccacgag caactggatg atttggagat aaagcattat ctctaaatgc
    27781 ttgaactgct tcaaagttta ataaacttgc ataatcttca ttttctagta attcaacttt
    27841 ttggatttca tgtgaagttc tgaatccatc aaagaaatgt ataaatggta atctaccttc
    27901 tattgcagct aaatgtgcaa caggtgctat atcagctact tcttgaactg aaccagaagc
    27961 taataataca catccagttt gtctagcagc cataacgtct tggtgatccc caaatattga
    28021 taacgcttga gatgctaaag cacgagcact tacatggaat actcctggta ataactctcc
    28081 tgcaacttta tacatgttag gtatcattaa taataaacct tgagatgctg taaaagtaga
    28141 agttaaagct cctccttgta aagaaccgtg gaatgttcct gatgcacctg cttctgactg
    28201 catttcaaca acattaactg tttgtccaaa tatatttttt cttccttgtg atgcccactc
    28261 atcaactact tcagccatag ttgaagatgg tgtgattgga tatatagctg ctacatctgt
    28321 aaacgcatag gcaacatgag ctgcagctgt atttccatca agtgttttca taaacttagc
    28381 cataatatgt atcctccttg atagctacaa ttgtacttgt ttataacaag tctatttttt
    28441 tatatttaca tataatttac acaacaaaac tataaacaca catatagtcg cgcaaatact
    28501 ctatcttcta atataatact acagattaga taaaattcct ttgaaaattg taaattaatt
    28561 acatatttaa gtagattttt tttaaaatta aatatgttaa gtttttttca taattttgta
    28621 tacaatatat aataaattaa gttagatttt tcaaactttt ttatctattt gttcatatta
    28681 gtataaaaag taaaatgctt atccttatta catgtaaatt tttatatata taattttacc
    28741 atattacatc atatttttgt aagtttttat tacatttttt ataatacccc tttattttat
    28801 aaattaatct aatcgattaa atttatccta tagaaattta ttacatttaa aaatatgcta
    28861 gcaaataaaa tatctactag cataaagtct atttataata ttaatcaact attaattcta
    28921 ctaaatctcc atttttaacg cctgctgcat ttccctcttc catatcaaca tgcatttcta
    28981 gattaaagct gtcactagct cttactaata cattttcaaa tactaatgct cttggtccag
    29041 atgttttaac ttttactatt tgcttatcag ttacaccaaa tttagcagca tcttcattgc
    29101 tcatatgtat atgtctagaa gcaactataa ctccttcttt catttcaact tctccagcag
    29161 gtcctactaa tttaacacct ggagttccag ctatatcacc agattctttt attggtgctt
    29221 ttactcctaa tgggaaacca tctgctaaag atatttcaac ttgagtatga gctctagctg
    29281 gtcctagtac tctaactcct tttaaagttc cttttggtcc tactaaatcc actttttctt
    29341 cacaggcata ttgccctggt tgagataatg gtttaaattt agttaactca tgacctgccc
    29401 caaataatac gtctatatca gcttgactca aatgaacatg tttattagat aatgctattg
    29461 gtaatttcat tgaaattccc tccttttaat tatttaaatt attgcaatac aattatacat
    29521 aatattacct acaaaatcaa attatattga atcaatatat atctttaaat ttttatatat
    29581 tatatcttta aattcttgat tatctatata agatacatca tctgtattgt ttaaatatat
    29641 attgtgtaaa agtttagtat ttatactctt aaagaaaaaa tccatcacaa tctgtccacc
    29701 tttaaactgt gaatcatatg atttactccc ccccactgca atatacattc ctatccgttt
    29761 cttacttcta tcaattgaag gtttatttaa tacatattta cttgcatata tagcttgagt
    29821 tctatcaaca acagtcttta atttagctga tattgaatca aaatgtacag gacttattac
    29881 tatagttcct tttgaatcat caaacatttt atataatggc ttcatatcat cattaaactt
    29941 gcaataccct gttttttcac aaaatccaca tgctgtgcaa tattcaatat ccatattgta
    30001 gatattaaat attttacaac ttaatccaca ctcttctagc atagctttta cttcatttgc
    30061 tataaaactg caatttttat taattcttgg acttccattt attattataa tctctggact
    30121 tttaagtcct tttttatcgt ttgaatccat aatcaatcta cctatccttt aacataacta
    30181 ttaaaatatc atcatcataa ttatcttttg atgagaagtt ctttaaatcc aattttagat
    30241 tttctactat taactgttga tttaatctaa aattttcttt tagaaagttt tctaatcggt
    30301 ttataccata ctcttctttt gatgaatttt ttatctctaa aattccatca gtgtacatac
    30361 ataccattgc ataatcttcc attgaaagag tattattttc ataagtagca tcttccatta
    30421 ttccaatagg tatgcccttt ttacaattta aattattttc tattgtgcca tctttcttta
    30481 caactacagg actatagtga cctgcattag acacagtaat catattttct ttagtatcta
    30541 taatacctat caaacaagtt gtaaatactc ccatcttgtc aaattcatca tataacatcc
    30601 tatttaaatt tgtcattatt tctcttggtg tattgtattg attgcataat actttaaaag
    30661 caccttttat cattgcaaca acataatttg ataccattcc atgtcccatt acatcagcaa
    30721 caatataaac tatatgttcg tcatccactt ttgtagcata ataaaaatct ccacctacta
    30781 cccttgctgg tttatggtaa aaataaagtt ctttctcatc attacaatcc attttacctt
    30841 tatccataat aagctgttgt tgtttattta atatgttcaa ctcgttttca agaactttgt
    30901 gttttacttc cttttcatta tgttcatata attgcatacc aacagaaact tgtttggaca
    30961 atatactaag aatatttaaa tcatcactag tatagttttt gctatttgca caaactatac
    31021 atcctatagt atcattttta tcctgaagtt tgtaatatat ataactttcc aaatttattc
    31081 ccttttttga gcatggtttt aaattattta gatacccccc caatgactct ttctttaaaa
    31141 acatatcaat cataggatat aaatttgttt tctttttttg ctcaacatta taacaactaa
    31201 ctatagcatc cttattatga tgactattta taattattaa agcttcttta ctattagtaa
    31261 tattacacgc ttgtttactt aaattttcca aaaagccatc aatactctta ttaaggtata
    31321 gttttttagt agcttcattt atggcactta ttgagcctct tagtttatca attaaaattt
    31381 gtacattatt atttttatta gttatttcta ttgataaact taagagtgat gcaacagatg
    31441 atataaaatc tatatcttgt tctgttatat aatcatcctc ttttaaaaaa caagtcataa
    31501 atccaacaac tacgttattt attataaaag gaaaaactat tctcccaata taaccttcag
    31561 cttttgctaa atctcgttca ccttctgctc tagaatcttc aaatacattt tccacatata
    31621 ttatcttttt ttcatttaca gcctcatgta tatactctgg atatgtgtca aaatcaattt
    31681 ttgcaccttt aagactattt attggaaaaa tttgaggtac atattctaag gtttgcgaac
    31741 atactaagta ggcatattta gaatcatttt tataaaatag attcacacat gcctttgtag
    31801 ggtgaacaac ttctaacatt ttttctatta tgtcatcttt tatattaaaa aaatctgtag
    31861 attcagtcac catagcggat atttccacta aattcttcat tttataactg ccattagtca
    31921 tactcaattt ctccccctat atattaaact atttattagt attttaacat acattcttta
    31981 tagtaaaaac aaaaacaacg acaaaactag caactaatat ccagttaaat cctgtcaata
    32041 atcctataag aagtaatgta attaatgcat ttagacctgt tctccctttt tcatccataa
    32101 aagttatttt agagatgtcg atattatttt tcacattatt tatatgctct ttcacttgtt
    32161 cttccatatt tcttttatat agtatataaa ctacaccaac taaaaacact cctataccta
    32221 aaaccatgtt taaattaaaa gctaccataa gcaatattga accaaaagct aactttaaaa
    32281 gcttaaaaaa tacactatct atctttagag ttatatttct gatttcttta cattcatctc
    32341 ttaaagaata tccacttgaa tctaagttat taatctttgt ttttacaatt tcagaataaa
    32401 aaagctttat tatcccattt aacattttca tcactcctaa atattttaca tatatttgta
    32461 agttttacaa ttataatatt ttttatactt ggtaataata aaaaataaca aatattttat
    32521 tatttagtat ttattgtaaa taagttattg aagcttgatt agttaagtat agatttttaa
    32581 taaatatttt tataatttag aaggaagtgg ttttgtgcaa atagaacaac tcgatttaga
    32641 aacgagaaat aaaatatact gctatactaa aaaaattctt agaaaatatc aaaaaggcat
    32701 aacatctgga aaattaacag cagataaatt tgcagataat atattgtctc aagattttat
    32761 cagtagtata cttaatgaaa aaatagtcaa tgagacaaac tttaagatat cttatagaaa
    32821 ctatatagaa actttaataa atattcaaaa tgaaaattta tctaacttaa gaaaaaaatc
    32881 tactaaaact gcaaaatgtt tcaatatctc tcaaataacc caattaaaaa acttactaag
    32941 taatactggt tataatttat taattccata taaatactta actgctagag atatagaagg
    33001 aatagttact cttattaata ctgggtctat tgaacttgga aatgagagaa tctacaatta
    33061 tatatcaaaa gtataataat ttaaacctat tataaaaagg caatttattt aataaaaaat
    33121 atttttaata ttaaataaat tgccttttta attttggatt aaatactgct atccaagtgc
    33181 tttttctagt ttagataaat gttgtattgg ataatatttt agtagctctt taaattgttc
    33241 tactgataaa ccttctgttg ttgtatatat tcttattttt tcatctaagt cactattaat
    33301 aaatttgttt tttaaatcct caaaatttac actactcatt ttacatcact ccttatgtaa
    33361 catattcatt tgtattattt tatccaaata cataaaattt attacataaa aatatatcta
    33421 tttaataatt taataaaaaa aagctatctc aatttagaaa ttactttcta tatttgagat
    33481 agctctagct aaactaatag ccaatagtta taaataatta tttataacta tctgttgctt
    33541 tttccagcca tttgttgttc agccatttca actaattttt tagtcatgta tccacctaca
    33601 taaccatttt ctcttgcagt aaggtttcct ttatcaactt gttgataatt actcatacca
    33661 agttcattag ctatttctaa tttcatttgg tttaaagcct cttttgctcc tggtactact
    33721 gttctgttgt tgttattgtt acttgccatg ttgattacct tccttctgtt taatttattt
    33781 tgtgttgctg ataatattat gtgattttaa acatttttaa ttcaggtaat ttttagcaat
    33841 taaaacctgc caaatatcca gtagaaaaag ctatttgcaa attgaatcca cctgtatatg
    33901 catctatatc tattacttct ccagcaaaaa ataaaccttc tactaatttt gattccattg
    33961 tacttgaatt tatttcgttt acttttacac caccagaagt tattatagct tcttcaatag
    34021 gtctgtatct ctttactgta aactttaaat tttttaataa atggactaag ttttttctct
    34081 gctctctaga aatttggtga acaaccgtat ttggattaat ttcacttaag tttattatta
    34141 taggtattag tttttttgga agtaaatcat ctaatgcttt ttcaaatttt ttatttgtat
    34201 acttctgaaa atctttttga actcttttat ccaatttttc ctcatctaat gcaggtttta
    34261 aatctaataa tatagtgtaa ttttctttcc ttgtatcttt cattctacaa cttgcagact
    34321 tgattatagg tccatctaaa ccaaatttag taaattcaag ttctccaaaa tcactataaa
    34381 ctttcttttg tttgctatta aacaccctta tttcaacatt tctcaatgat aatttttcta
    34441 aatcttttgt aaaactttct tgtacttcta taccaattaa agatggtttt gtatctataa
    34501 ttgtgtgccc ctgtgaaatt gcaaatttat atccatcacc agtagaacca gtaagtggat
    34561 aacttaatcc accagttgct actacaactg aatcacactt aatttctttt ttatcgttta
    34621 aaattacttt ttcaatttta ttatttttag atattatttt ttctactttc gaatttaata
    34681 atatatttac tttcttactt ttaagttgtc tttcaagagc atttacaata tcaaatgctt
    34741 tatcactctc tggaaaaacc ctttttcctc tttctgtttt agtctttact ccaagattat
    34801 taaacatact tattacatca tcatttgtaa aagtataaaa agcactataa agaaattttc
    34861 catttgtagg tacattttct attaattcct ctatttcaca agcgttagta atattacatc
    34921 taccttttcc tgttattgca agtttttttc ctaatttatg atttttctca attaatgtta
    34981 catcatagcc tttctcacat gcagttgatg cggctatcat ccctgctggt ccaccaccta
    35041 ttattactac cttcttcaaa taatcactct tttctaccct taattttttt aatttaaact
    35101 attgactcta taccttaaaa tcaatttaat tttaaaagtt taaataatgc tggagctcct
    35161 gctattccat aaaaaacaac tactgtattc ttaataaaat tcataaaaat tatactcata
    35221 gtagagtcac ttatatttaa agtgactatt aacattgaaa taaacaactt aatgccaata
    35281 tataacattc ctattgttac tatcccaacc aaaaacctac atattcttga aaaaattata
    35341 tttttactcc ccttattttt attgctaaaa tttatttttt tgttattatt atctgtatta
    35401 aatttaacaa atctatcctc aaccatatat ccaaatacaa atcctactat tataccaaat
    35461 ttttcaaaat attcagggct atttagaaat atagccacta ttataaatgg tataagtaaa
    35521 actacaagta aaatataatt tttattatca tcaatatagt caaatatttt tacaaataca
    35581 actgtaaaaa atattcctaa tatccaaccc gctatcacat ctattggcca atgaacagct
    35641 aaatacagcc tagacaatcc tactcctaaa accataactg cacctattaa atgaagatat
    35701 ctctttttaa atatagtcat aatactaacc caaaatgttg ttgcagtttg agtatgagca
    35761 cttggaaaag aataaccacc agcagtagaa actctcaatg attcaagacc ttttattcct
    35821 attggtctgg gagcttttac aaactctttg atgcctaaat ttacaacaaa atttccaatt
    35881 atagcaaaca gtattttctg tccacatttt ttatttatac accagtatat aattgttgta
    35941 attaatatta ataacggtgc ttctgtgctt attgtaaaga caagaaatat aaagttcaaa
    36001 attggatttc ttaaattttg gaaaaataat aatatattta actgaatatc catatagatg
    36061 tctcctttac taaaatttta tataattaac taaaaagcac taactccctt agaaattagt
    36121 gcttttgatt cacatttaaa tctattctac atcctcaact atacctatat aaggtaaatt
    36181 tctatacttt tcagcatagt ctattccata accaacaata aatttgtctt ctataacaaa
    36241 tccaacatag tctacaggta tattagcttt tcttctctga ggtttatcaa gtagagtaca
    36301 tagttttaaa gattttggat ttctagtttt taaagctgca actagattac ttaatgttaa
    36361 accagaatct attatatcct ctacaatcaa cacattcttt ccttcaatat ctacatctaa
    36421 atcttttaat attttaactg ttccagaaga ctctgttgag tttccataac ttgtaacact
    36481 cataaaatct atatttacat ccaaatctat acatcttatt aaatctgaaa caaatacact
    36541 agctccttta agtataccta ccactagtaa atcttcgcct ttaaagtctt cttctatctt
    36601 ttttcctaat tcataaactt tttcttttat ttgttcttct gtcaacattt ttcctgtaac
    36661 tttgtacatt ttgtatcctc ccgctttcct tctattaact tatatattat agcataccaa
    36721 aatatcaatt aaaatctttt tcatcatttt taaacctaaa ttaacagctt atccatactt
    36781 ttttgctttt tacattaaaa atttggaata acgattcctt tgtattataa tctatatgta
    36841 catatattaa tcagttattt ttatcgatta tataatttta tttttggagg tacttttatg
    36901 agcacaaacc atggagctaa tttatatagt ttatctagta agtatggctt ttcaaaggaa
    36961 gaatttatgg attttagctc aaatataaat ccttttggta catcaagttt agccaaacaa
    37021 tacattgtaa acaatataga tatggtatct atgtatcctg accctgatta catagactta
    37081 aaaacttcta tatccaatta ctgcaaatgt tctatagata atatagtact tggcagtgga
    37141 gctacagaac taatttcatc atttatacat acaattaacc ctaaacaggc tcttttactc
    37201 tctccagcct attcagagta tgaaaaagag ttatctaaaa ttaattgttc tatagaaaaa
    37261 tactttgcaa aagaagaaga taattttcat ataaatttag aaaatcttat aaaaactatc
    37321 aatgctaaag attatgattt ggtagtaatc tgtaatccta gtaatccaac tggatttgca
    37381 tttactaaag tagaagtgag agaaatttta aaaaatacag actcattttt aatgattgat
    37441 gagacttatg tagagtttac tgatacagat acatactctt gtactcaatt agttgatgat
    37501 tattctaatt tattcgttat aagaggtaca tcaaaatttt tttcaacgcc tggaataagg
    37561 cttggatatg gtcttatatc taacactaat gtcaaaaatg aaataaataa aaatctagat
    37621 ttatggaata taaacataat agcttctaaa atgggtgaaa tcatgtttag tgatttagat
    37681 tttatatcaa atactatttc cttaatgaac actgaaagag attacctgct taaagagctt
    37741 aagaatataa aaagcttaga tatatacaat actaaaggta atttcatatt gtgtaaaatc
    37801 aagacaaagg aattgactgc taaatctctt cgagagcaac tgcttccaca aaaaataata
    37861 ataagagatt gttgttcatt tgaaggtcta gatgagtatt tctttagagt atgtatatta
    37921 aaacctaatg aaaataaatt attaatatca tctcttaaag caatttttaa ataaaaaaat
    37981 cactttattt tataattttt tataccataa aaaatgagct atctctattt gagataactc
    38041 atttttgatt attaaaaaac taagcacttt gctcttcttt atttcctttt ttactcattc
    38101 catcatttat tttatctact actactgcta ttgcattatc tcctgataca ttacatgctg
    38161 taccaaaact gtcttgagtt aagtataaag ctatcattaa acttgcaagc ccaccatctg
    38221 atggtattcc aaccattggt aggaaaggaa gtgcactcat tatagcccca cctggtgctc
    38281 ctggtgctgc aaccattgct atacctaaca ttgcaatata agggaataat acacctattc
    38341 catgtggcat accattcatc attaaaactg cagttgtaaa tcctgttatt gatatcatac
    38401 tacctgataa atgtattgtt gcacaaagag gtacaacaaa ttctcttatt tgcttactta
    38461 ctccattttt ttctgcacaa ccaacattaa ctggtatagt tgctgctgaa gattgagttc
    38521 ctatagctgt taaatatcca ggtacttgat tttttaacat tcttactgga ttttttcctg
    38581 aaaatacacc tgctgctgtg aattgacatg ccatatatac aaaatgtaaa ggtattacta
    38641 ctaagtatac tttccagaat acccctaata ttgtccatac ttcccctgct atagttatat
    38701 ttgcaaaagt tcctgctatg tataaaggta ataaaggtat aatcactgtt gacaacgttt
    38761 tcgttacgat tccttgaaat tcttggaaga aattaaataa agtctctcct tttccatgat
    38821 ttcttaaagc tgatattcca agtcccatta caaatgcaaa tacgattgca gctgttacat
    38881 ctaccatagg ctctaatgga attgtgaata ttggagctaa cattcctgcc tctgggtctc
    38941 caatttgact tgcaacactt gcatcaataa aatttgggaa gattaatgta gatactgcaa
    39001 atgataataa tcctgctatt aaggtagaac catatgctat tcccgtagtt atccccaata
    39061 actttcctgc acctgttgct aaatcagcta tacctgctgt tacaaaacct ataattataa
    39121 atggtataac aaattttagg aatgctgaga ataaagtact aactgttact aatatttgta
    39181 ctattatttc tggtgcataa cttccgatta aaataccgac tatgataccg atgattagtt
    39241 tgggcactaa acctattttt ttcttcataa tatttaccct ccataacttt tttaaattaa
    39301 gcattcttgc acattgaata cttagttaaa tattggatat ccacttatta tgataataga
    39361 gttttacctc tccgtcaaca atttaaccat tttttgttaa taaaaaaaag aataacatat
    39421 ataaattgtt attcttcttc atcttttata gaaaattcta cattttcaat tatatttgaa
    39481 tgtttatctt tttttgcgat aactctatac ttattaattt cttcattcac tatacctata
    39541 ctccaaatta aaactatagc atcatctata aaaccaaatg cccctgcaat catctctgga
    39601 attatatcaa ccccagatac aaagtaggat atagtaaaaa ttaatacaga aattaccttt
    39661 attttatcaa ctatattgat tcttttatct gtcataaaat caggtagttt aagtaaatca
    39721 aatacaaaaa ccaaaccaaa tttagcattt ttaaatctga ccaaaatttt ttttaaaata
    39781 tctaaaacta tattaatgat tatttttaaa aactccatat ttatcacctc ttataattat
    39841 aggttattct acattataac cgatataatt aatttgatga acatattaga tataaatttt
    39901 atcaattagt ttatatttaa attataataa ttcaattcct gcactactac aattttgaat
    39961 catatcctgt ggccatactc caacttgtac ttctcctata tgagcttttt gtaagaaata
    40021 catacatatt cttgactgac caattcctcc accaattgta tacggaagtt cgccatttaa
    40081 aagcatctta tgatagtcaa actcttttct ctcttcacat ccagcaagtt tcaattgtct
    40141 atcaagagat tcttcatcaa ctctaattcc cattgaagat aattctaatg ctgaatctaa
    40201 aacaggattg taaaataata tatccccatt caattcccaa tcatcataat caggacttct
    40261 tccatcatgt ttttctcctg atattaatgt ttttcctatt tgagttataa atactgcttt
    40321 cttctctttt acaattttat cttctctttc ctttggtgat aattctggat acatatcttc
    40381 taactcttgt gatgttataa aagttatttc ttctggtaat atttctttta taccttcata
    40441 catactacat acaaaaattt cagtatcttt aaatactcca tacaatcttt ttacaacatc
    40501 ttttagagtc ttttcatttc tactactttt atctataacc aattcccaat cccattggtc
    40561 tacataaaga gaatgtatat tgtctaattc ttcatttttt cttattgcat tcatatctgt
    40621 atataaacca ctaccaactt caaacccata ttttttaagg gctaatcttt tccattttgc
    40681 aagtgattga acaatttctg catttttatt cataaatggt atatcaaagc ttacagcctt
    40741 ttctccattt aaattatcat ttgcacctgt ttctggcaat acaaataacg gagaagaaac
    40801 tcttataaga tttaattcat tacttagtct catttcaaaa aaatctttta atttttttat
    40861 agctatttgt gtatcaatta cgttcaaact acttttataa tcctttggta ttattgtgct
    40921 catatcattc actccctata tttatatatt tttccattaa aaaaaccctt catccctata
    40981 taaaagggac gaaaggttgc ttttccgcgg taccaccctg attagtttga aataaattca
    41041 aacccagctc acaatgtaca gatattactc gatacacccc aatatgataa cggtttggtt
    41101 ccgactaagc ctactctttt aaaatttcgg ttagcaactc caggatgttc ttcactatag
    41161 acttcatatt ggtctcacac catcaccaac tcgctttaat tacattctat aactactctt
    41221 cctttcaaag tctttaagag tttaattcaa taaaatttag ctctcttatg ttttctattt
    41281 attcataata gtatgataag tgattagtta aaatttgtca agtttattat ttttttattt
    41341 ttaaacactt tataaatttt tacctatact ttataaattt tcaaatatag tctttaatct
    41401 gtccatacat tcttttaata aagactgtgg acaagctaca tttattcttt caaatcctat
    41461 tccttctttt ccaaatatat aaccttcatc aaataataca ttagcttttt taaacattaa
    41521 atcttcaagt tctacttcat ttaatccata agcagtaaaa tctaaccatg ctagatatgt
    41581 tccttctgga taaacaagtt ttacttttgg taggttttct tttaagtatt catcaataaa
    41641 ttgtatatta ttatctaagt aatcatttaa ttcattaagc cagtcttcac tttcattata
    41701 tgctgctatt gtagctacaa tagcaaaggg atttggtgaa gttgcaactc caacagctgt
    41761 aacctcttct tgatatttct tttttagttc atcattattc actattatat tagacaactg
    41821 cattcctgcc agattaaatg ttttacttgg agctatacat gtaactattt tatccttgta
    41881 ctctgggcat aatgactgta aaggagtatg tttaaatcct tttctaatta aatcagaatg
    41941 tatctcatca gctattatcc ataggtcata ttttttacat atttctacaa ccttttttaa
    42001 ttcatcttct ctccaaactc ttccaacagg gttgtgagga ctacaaagta ttagtacttt
    42061 attgttgcta cacttagctt tttcttctaa atcatcataa tccatcctat aagtaccatt
    42121 ctcatatatc agaggattgt ttacaacgtc cctattattg ctctttatct tagcttcaaa
    42181 tgggtaatac actggttttt ggactattac accttcatta gaattagtta atattcttac
    42241 taaaatagca acagcaggta caacaccagg actaaaaata atatcttttc tacttatttc
    42301 ccagttaaat ctccttttat accaatcaca aactgcatta aagtatttat cactatcatt
    42361 tgaactatat ccaaatattt tatgttcaac ttttttacgt aatgcttcta ttattggttc
    42421 tgcacattca aaatccatat cagcaatcca aaaaggcaaa ccatcttttt gtgcttttgt
    42481 tgaacaatta gttctaaaat cccattttac acaatctgtt cctactctat ctattactct
    42541 gtcaaaatta tacttactca ttttttccct cctaagaata aaatttttat aattgttaag
    42601 tctattacta gatgcacttt ggtattattt tacaattctt aatattataa taatatcact
    42661 tttttgtcca atagatttaa aatataatta cttttccatc atattttttc aatattccaa
    42721 aatcagtttc attatatcaa aataagattg ctcatattaa ttattttcta attaacataa
    42781 gcaatctttt tctataactt ctataattat aattttaaat gtagaattat tagtttggat
    42841 gttatcccaa tctcttttca accaatctac accatatatc tacaccatat tctaaagctt
    42901 tcgtatcaaa tgttacattt ggattatgaa atcctggtgt agcatcagct cctaatccaa
    42961 tatatgtagt tttacatttt agtttttgtg tataataatg gaagtcttct ccaccagaat
    43021 ttttaaatat acctatagat gcatcagctc ccaatacatc ttttatagtt tcttcagcca
    43081 agtttacaag ttcttcatca aattctgctg ctggtactcc atctttggaa attagtacag
    43141 cttctgcatt tatagaactt acaacagttt ttacagcatg tattgctttt tcttttaatt
    43201 cttctattat atcattactt tctgccctta tatctaaagc cataaatgct tcatctggta
    43261 tattattata agtttctccc ttaactgata tatttgttac ttttatagaa tttggtatag
    43321 ctggatttac tcttattgca tttattgcat tcactgctaa acagacagca tctactgcat
    43381 ttactcctaa atgtggttta gaaccatgac aagtgatacc tttaatcgta aattccatta
    43441 tacagctagc acaatggtac attgcaggta tcgcctttcc caacctacaa tcttgtccag
    43501 gtcttaaatg catccctacc atttcttcta catcatccaa caaaccagaa tcaattactc
    43561 ttaatgctcc atataatttt tcttcacctg gttgaaaaag tatttttaac ttacctcttt
    43621 ttactaaatt tctctctttt attaactttg cagctgttaa aagcatagaa gtatgaccat
    43681 catgaccaca agcatgtatc attttctttt ctccatctat gataaattcc aatgcatcca
    43741 tatctgctct tatagcaaat actggtcctt cttttttaga atctatagtt actataatac
    43801 cagttcctgc aactccttta ttaatatttt caaaccccat tttttcaagc tcttttaaaa
    43861 caaactctga tgtcttatat tcttcaaatc ctttttctgg tatagtgtgt agataatcat
    43921 acactttatg tacatactca atataatctt tgttagttcc cataatctag ctcctcctta
    43981 aaatttaaac tttccattta tattaaattt ttattataat tacctatact acgaaataat
    44041 tcattataag cattgcaata caaccattta aagttgctat aacaaacata tgtggataat
    44101 atttagtttt taactcagct gttcctaaaa gtctccccat aaattgtatc tgcgctcctg
    44161 aaagcaataa cattggcaat agaattgcaa catgtgcttg acttatttga cctgctgtaa
    44221 ataaacctgc gattactcca gatgctcctc ccattgatat aaatattgtc attatagctg
    44281 ttgctgcaac acctggaagt ccaaagatac tcattacagg tgtaaatatt gtccctatta
    44341 catccaaaag acctgttaag ttgaacacag aaattatagc aaaaccgaag atgacatttg
    44401 gagccattga atttgtactt atttggaatc catttctagc tcctccaata aacgcatcta
    44461 tcacatttcc ttttttaact tttacttctt tttttgctac ctcactcatg ttaagcttcc
    44521 cccttgtcaa atttatttaa atataatcta agtaagttcg ccccaacaaa cttcataatt
    44581 aatattaaag caagtggtat aaatattggt acatctccta taaatggaaa tagtgctgct
    44641 ccagaagcaa agaaattcgt aattgcagac gcacctgaaa attgaaatgc acaaaataca
    44701 gctttttgtt tttcatctat atacccatca tctttaagtt ctttagtcat agatgcagct
    44761 gcatctgtag actgtaaact agctatcaaa gttagtcctg caaatccagg taaccctaat
    44821 aatggcttta atagtggact aagaagcttt tgagctgcct tcattccatc taagtggtct
    44881 ataactctaa ctactccaag tgcaaacata actgatggta ttaatgtcaa tgcatatagc
    44941 catccatctc gagggccagt acctccatca cctctaaagt ttgcagctaa tgtacctaca
    45001 ccatcattaa ttgttcccaa tgttccaaaa gaacctaata cattgttaaa atccaatact
    45061 tttaatggtc ctggtgcatc tttaaatatt cctgaaaaaa ataatgctcc caatattaat
    45121 gctacatacc cataccattt tacctcaact ttttctgttg ttgattctaa tttactcata
    45181 taaattctcc ctcctgttta aatgaatttt attagtatac ttgtttaata agcaaaactt
    45241 atgccaatac cttaatgata aagcctttgt atttattttt ttattgtaaa tttattccag
    45301 tgattaaata gcttataatt acttactttt caagtttaaa aatataatat ttttttatta
    45361 attatttaaa atatacatta caatataaaa agctattttc cttatcttgc aaaaataatt
    45421 tcaaaatagg tttttatagg ataaaatcct aaaaaaacct attatttaat caaatatata
    45481 aatttatact actatttgtt gtataatata acttgcctta tactttaaat actttaaaaa
    45541 taaatactat tttgttagga gaaataaaat ggataaaaag aagttaatcc ttataacata
    45601 tgataaattg aatagtgacc actacaaaga agaattaaca aacttttttg gagacgaaat
    45661 aatcatagaa acacaaaata tacttgatgg aataaaagaa aatttagagg gagatgtagt
    45721 gctttctcta tcacctttaa ctagtaattt tttaataaaa cattttaagg aagatattga
    45781 aattattcat ggaacgaaag cactttcaaa acttggatat gaaaaaatga tgaaactgcc
    45841 tccaggtaca aaatcgttac ttatgacaac caataaaact tcagcatttg agatggctac
    45901 ttatctatat aaaattggta taaatcatat agattttgta ccgacatatc cagattgtga
    45961 tgaaatatat gatttagata cagctataac accaggtcag attagattta taccaaaata
    46021 cataaaaaat attgttgatt taggttggag aaaaatctca ttagatacat atatgtcatt
    46081 attagtagta ttaaaactaa aaaatgagaa atttatagaa aaattataca aattgtccaa
    46141 ggagacacta agtcatgatt tcttaaatac atcattagat aatatatcta aattaaaaac
    46201 tatactttac atgaccattg atgaaatagg tgatggtcta atatttttca atacctttaa
    46261 taaggttact tttgtaaata agtctttact taatatgttg gaactagatg aaaaacttat
    46321 aaaatcacct tccttaatgg aatatatgcc aaagagtttc ttagataaaa ttacaaagaa
    46381 cttaaatata gataatatga ttatatacat agacgaaata gataaaaaat tcatactttc
    46441 taaaaaacca ttttatcttt ataaaaatat agagggttgc ttaattacat taaaagatgt
    46501 aaacaatata gaaatattag aacaaaaaat aagaagtgat tctgtcaaaa gaggttatgt
    46561 agctaaatat aaatttaata atattattgg aaatagttct attataaaag attgtattaa
    46621 aaaggcaaaa aaaatggctc ttacagataa tcctatatta ataacaggtg aaacaggaac
    46681 aggtaaagaa gcctttactc agtcaataca caatcattca aatagaaaaa ataaaccttt
    46741 tgtagcaata aattgtgctt cattaccaag tgaattactt gaaagtgaac tatttggata
    46801 tgaagatggt tcttttactg gagcaaaaaa aggtggaaaa aaaggattat tcgaattagc
    46861 tcatactggc actatatttt tagatgagat aggagatatg ccacatgatt tacaggttaa
    46921 acttttaaga gtcttacaag aaaaagaaat tagaaaaatt ggtggtacaa gtattattcc
    46981 aattgatgtg agaatattag ctgccacaaa taaagattta gagaaattaa ttatagaaaa
    47041 taaatttagg atggatttat tttataggat aagtatgttc actttagatt tacctcctct
    47101 tagaaagaga ttagaagata tacctcttct tttagaatca tttttaaaag aacttcccta
    47161 taaaaacata aaactcgata agagtctttt agaagcttta aattcataca cttggatggg
    47221 gaatattaga gaactgagaa attgtgttga atatatggct tacatgggtt ccaattatct
    47281 tacaataaat gatttacctc aaaacatatc ttcaaaatta aataataatc atatgtcaag
    47341 taatatgtcc atattcaatg atttaaacca atatgacaaa aacatatgca tttctatcct
    47401 aaaatcattg cacatgaaac caatggggag gacaaagtta atgaaattta tggaatataa
    47461 tgtcacagaa tacgaagtaa gaaatatgct agaatatttg acacgcaatg gatacttaat
    47521 ttctagtaag ggaagaaaag gctcatcaat aactgagaaa ggcataaaaa taatagaaaa
    47581 taatataatt taaacctata gtaatt
//
LOCUS       DAYASV010000016        46315 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYASV010000016 DAYASV010000000
VERSION     DAYASV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 46315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.016"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            465..587
                     /locus_tag="ACGI68_000917"
     CDS             465..587
                     /locus_tag="ACGI68_000917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137365.1"
                     /translation="MMDLEKEKHEIIEIIKKLNIDKDKRNLLMIKNYILGLLKK"
     gene            complement(602..988)
                     /locus_tag="ACGI68_000918"
     CDS             complement(602..988)
                     /locus_tag="ACGI68_000918"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137366.1"
                     /translation="MIDEMKPENKRIRELRLNLNLSQEEFANKLNLKRNTISLIENGK
                     RNASERTLNDICKTWSISEEWLKHGVGEMFFIKELDEQFAHLVGELSKMENEVEREII
                     TKMFKLNKKYLTVFDKMIDSLLEIEE"
     gene            1136..1279
                     /locus_tag="ACGI68_000919"
     CDS             1136..1279
                     /locus_tag="ACGI68_000919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137367.1"
                     /translation="MTDINKIREEIYRKLELLGPAKLKLALTKVDILKEIQDIEDENN
                     IQN"
     gene            1284..1508
                     /locus_tag="ACGI68_000920"
     CDS             1284..1508
                     /locus_tag="ACGI68_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137368.1"
                     /translation="MKRLKISDFNKNQIGSAVQLQDIRFDTKADCIITKVEKNEILVM
                     YYEKETEEIAYKTLTKEDLILDDYKLKLLS"
     gene            1527..2267
                     /locus_tag="ACGI68_000921"
     CDS             1527..2267
                     /locus_tag="ACGI68_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137369.1"
                     /translation="MITLDLLKDARKKEWFWLENDLIDREDLSIYEKMIYIVLARYSN
                     EESSCFPSYKTISEKAGCSIRQVSNILNELENKELIIRENRNHEGKKEKNSNLYFLIS
                     SKAKVQNEVPNVRHEVPNPMAPGALHVRHEVPSKKTYNKKTYIKSNTTTQKESKTDYL
                     DLSFLDLDIEKVKLTKDEYDKLISKFGKKYIHDKIVSLENYIINGKGSRYKSHYRALL
                     TWGNGDASKGIVPEVTKTKNPLSGFKEL"
     gene            2295..2480
                     /locus_tag="ACGI68_000922"
     CDS             2295..2480
                     /locus_tag="ACGI68_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137370.1"
                     /translation="MNEKEETVYLNSSNVKLFLGVRDIKEFEELIENVNERICQLQES
                     INKLAQFNIEFDIKSDI"
     gene            2506..2724
                     /locus_tag="ACGI68_000923"
     CDS             2506..2724
                     /locus_tag="ACGI68_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137371.1"
                     /translation="MKDEKLELTEKDLHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECKDYFTHIDTFIKLSEMTGVDIFTK"
     gene            complement(2743..2949)
                     /locus_tag="ACGI68_000924"
     CDS             complement(2743..2949)
                     /locus_tag="ACGI68_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137372.1"
                     /translation="MCKIKTLREKCSLIDDKVEFSGEYKTFPGFTGSKLLECECNKNY
                     ICKIQIMTANSNCPLFKKARQLQY"
     gene            3118..3378
                     /locus_tag="ACGI68_000925"
     CDS             3118..3378
                     /locus_tag="ACGI68_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137373.1"
                     /translation="MKEEKLELTEKELHCIARHLQNEVMEMVFRGNIEAPTSCEVCDY
                     LQECEGDFTCMYHSFTKLSKITGVKVTAFKYCFPDENPKATI"
     gene            complement(3371..3559)
                     /locus_tag="ACGI68_000926"
     CDS             complement(3371..3559)
                     /locus_tag="ACGI68_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137374.1"
                     /translation="MSQYRVISKECPVINETISLTCEYSAPPGTFGSKLMSTRCDGSH
                     ICDSSSNEECPILKKLLR"
     gene            3697..4014
                     /locus_tag="ACGI68_000927"
     CDS             3697..4014
                     /locus_tag="ACGI68_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137375.1"
                     /translation="MERKKHEFTDQDIQRLIEGLRTDPKIREMIFKSKGCETRTSTRS
                     KCEQFVEEKLAYRSYGFETPKYYAKLIADIIDILVYKNVPRDRICGVLYDVERVIPLV
                     VTF"
     gene            4036..5367
                     /locus_tag="ACGI68_000928"
     CDS             4036..5367
                     /locus_tag="ACGI68_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFH0137376.1"
                     /translation="MNYSLEAEQNILGSFLINDSIGYKIRELKENDFYFESHKIILSC
                     MKKIIDSNKPLDLLLLKNELEKIDRLVDIGGVSYITSLTTIVITTSNIDHYIKIIKEK
                     LLKRQISELANELINNSNSDTSIDELIIDINDLKALVTTSSSVNDNYIDASKIKREKG
                     VHKSIDTGFNKLNNMLDGFRYGTLTILTGKPASGKSTIVNQFIAQAITNGEKAFLYSG
                     ELPSFMSMDWFRKTVANDYHIKEYKSVYGGTYTDIPDYAVELISDWIEDKFFLYDEDA
                     ISDEVNLLNTIEHLYLKKGVRFFVLDNLMTIKTGNKADKYERQEQIVSNLKNLAKKYN
                     LVIVLVAHPRKNMGDMKPTMYDVSGASEIVNYADYILSTYRVVDEEEETDDTYLLILK
                     NRITGKQNISFKMNFSERRKRLYTTSEELNRDYKYDVNKQYVQVEIPEDVF"
     gene            5742..6128
                     /locus_tag="ACGI68_000929"
     CDS             5742..6128
                     /locus_tag="ACGI68_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137377.1"
                     /translation="MIALEKFAGGVLKEKFNTELQKVLDNIADPNTDFKKTRKISLEI
                     VFKANEDRDLAEVDIKSKATIVEAKATTTKVLIGKDLETGRVEASEFKNQVAGQLVMD
                     IPDSEETNETEENSAGVIDFRNASSK"
     gene            6181..6885
                     /locus_tag="ACGI68_000930"
     CDS             6181..6885
                     /locus_tag="ACGI68_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137378.1"
                     /translation="MIRNAMEYLVKLGKRETVCVDGFKYTTDALEIIKEPKANELEIT
                     TLSGLVDYIKSGIDHKADELLLIQVLTPRKVLLKSPLRKNRDRETYIECVALLPEKRF
                     DRPLDLDSFNIMLQSAFIKNNDRDTLLKIAGNIQESTVKNVGDDGVSQSVTIKTGVAS
                     VSEAIVPNRVKLIPYRTFQEIEQPESEFIFRMSSGPAAGLYEADGGAWKNQAMLNIKA
                     YLQEELKDFKNVNIIA"
     gene            6942..7124
                     /locus_tag="ACGI68_000931"
     CDS             6942..7124
                     /locus_tag="ACGI68_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137379.1"
                     /translation="MKMISHMNILERAEFTDKIANAIMSANKEIERGCAFNEAVEIVK
                     QMEYRENKYNEGDIAI"
     gene            7121..7498
                     /locus_tag="ACGI68_000932"
     CDS             7121..7498
                     /locus_tag="ACGI68_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0137380.1"
                     /translation="MNNVVLVGRLARDPELRYIPEYGTPVATFALAVDRGYTKKDGTR
                     EVDFIPIEVMGGSAEFCANYLAKGRMVSIQGQIRIEKYEKDGEKKTFTKVRTKIVNAL
                     DRKPKDEEKEISFQVLDDEDIPF"
     gene            7476..8063
                     /locus_tag="ACGI68_000933"
     CDS             7476..8063
                     /locus_tag="ACGI68_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0137381.1"
                     /translation="MMKIFHFKLDGDYVRSLEERNKLVEENINFVWFIIRRYFKVFIS
                     KYPYLQDDTFSEGCIGLCKAADYFDSKKGIEFTTFAAVCIKGQIFKFISRYVKKHYRA
                     DIDSFEKCIYKNTHGEEIRIEDRLAGNENVDIENVHVIRSCIKRSKIEDIQKITYLRE
                     KGYTQKEIGKILRTNQMTICRKLGKLKSEINILGK"
     gene            8093..8251
                     /locus_tag="ACGI68_000934"
     CDS             8093..8251
                     /locus_tag="ACGI68_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137382.1"
                     /translation="MKTKIIGGRENQCPLCNGSVFKIETSIGIVWQCKDCGCMYQDMN
                     CKENKRRH"
     gene            8254..8580
                     /locus_tag="ACGI68_000935"
     CDS             8254..8580
                     /locus_tag="ACGI68_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137383.1"
                     /translation="MACVIKCDFCGETIAEILNIKVFRIDDEGNIMSIGKDVCDKCYD
                     RLFRSNLNKKMTNYEKIKNMSKIEMAEFLANGGSGCTSCAYDFQECLGGCLEGRKKWL
                     ESEAMK"
     gene            8580..9116
                     /locus_tag="ACGI68_000936"
     CDS             8580..9116
                     /locus_tag="ACGI68_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFH0137384.1"
                     /translation="MKKTLIIVGVVISILIMFACLFLVTKNKAIDLEEQVQSATASVE
                     VQEKRRIDLIKNIVDCVKDYAKYENRTLKEVTDMRVSANDESIKEAKLNIQAVAEKYP
                     DLKANENYKQLMSELSITENMIAQHRENYNMQVRNYNKYIRKFPANLILNSMGYEKIE
                     SKYTEYKAKEDAPQNLFD"
     gene            9128..9934
                     /locus_tag="ACGI68_000937"
     CDS             9128..9934
                     /locus_tag="ACGI68_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137385.1"
                     /translation="MKFKTFEITKREFLVSIIIACMMIVLGFFIDDKVQSKFLEDNER
                     YYKATKINNDKDLFDYAMETSAGDSLISGRFKSVDSVSIEELKGDYWFIERTEEKYTM
                     HTRVVTTKDSKGHSHKYTETYWTWDYHDSIEKKCNKFNFMGKEFDAKVITNLSKERLA
                     LKKDIFKNNNLKIKGNYAYINNKRRYYYEVIPKEFNGTMYANLKNNTIANPADKNIKV
                     FKGESIQNVLENNNFNTGFFKVVFWIIWILITTIIIYVFYYLKNDWLEDK"
     gene            9959..10219
                     /locus_tag="ACGI68_000938"
     CDS             9959..10219
                     /locus_tag="ACGI68_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137386.1"
                     /translation="MKCKVYHAIKNIKDANELIDRFETENIFFELNDIDNENVLNGEM
                     NLTDIEDIASLQFILDGDLFIFSSCDEMIFMNKYTQKELGLQ"
     gene            10243..10728
                     /locus_tag="ACGI68_000939"
     CDS             10243..10728
                     /locus_tag="ACGI68_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137387.1"
                     /translation="MDLKLISSIPNWKVEDQLAKIDEEVTEFKNAIDTGITKDIIAEG
                     LDVCQTILTMFQVLEIENYITEGVETHNKKLNGRGWDLKEIGDDILNLIKNSMINELI
                     NMNKKLKCLDEKEFNAKIQFLDVDKLWNLCMPLVNLSTIKGVNESIDEMKMGEFLKNI
                     N"
     gene            10748..11005
                     /locus_tag="ACGI68_000940"
     CDS             10748..11005
                     /locus_tag="ACGI68_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137388.1"
                     /translation="MLIEVTENNRELKVGDIVEINGGNKLYMILSLKETIGGYVIINM
                     QNGYGSFGAYKSLSQLELDLKVRGYKLYSSDDYKLQLVPRE"
     gene            11114..11557
                     /locus_tag="ACGI68_000941"
     CDS             11114..11557
                     /locus_tag="ACGI68_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFH0137389.1"
                     /translation="MQSSKKDNLFSSAEGKLYDYKKIKAEIEKIDIDMQMIKNDYRGC
                     SGVEIKEKTGKTYDIKSVIEIETEEKEKKIALKEKEKRHKELIIEKIDNAMKILSEEE
                     KKIVQYKYFSSSRTSWEYVGRMIGFSASKCKQMRFDIIDKIKGLL"
     gene            11700..12245
                     /locus_tag="ACGI68_000942"
     CDS             11700..12245
                     /locus_tag="ACGI68_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137390.1"
                     /translation="MAWNLRSKSFLLSREVLKIKRDITEEISSAAYVPDNLKYYDNVM
                     KETPGMYGRECSLDFIKKKQEKLLKLKKKVKKNYDSNINKIDSYLKILEKSIIDESDH
                     VELVVFKLYLQLENVVKVTETVNDLGFRIKTNTYARERKYSTNDITSIITDPFTDVVE
                     DLKILVQERQRKNYYGNKECF"
     gene            12249..12893
                     /locus_tag="ACGI68_000943"
     CDS             12249..12893
                     /locus_tag="ACGI68_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0137391.1"
                     /translation="MEAHSGQKVNPIKDIEDVFRLLNFLEEWNERNYLLALFGMCTGL
                     RVGDILALKVADVTDLKLDKKGNKIRVSKDWIRVIEEKRDYNREVFLSDVIKSAIENY
                     TQDKPGEEFLFKSGKRKKFNRPIQTRQAGRIIKGAAKKVGIKENVATHSLRKTFARHI
                     YDEEENKTYALELIRKILGHKTIEMTRRYIGIDKDEEVKAITKFTNKLKKRKRN"
     gene            13114..13566
                     /locus_tag="ACGI68_000944"
     CDS             13114..13566
                     /locus_tag="ACGI68_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137392.1"
                     /translation="MNEVEIIDSSYEKGKELTEDQCNMVTLLITGATVTETARIIGVN
                     RKTIYNWMNKEHVRKEMDRRKQELTNQGNLMILKDLDSYINNIKELASDKSDKRVMLA
                     ANQYLINRIYGTPTSTVIQAEDNSVGMGMDATEIEAAIAKIRIRTSKK"
     gene            14121..15860
                     /locus_tag="ACGI68_000945"
     CDS             14121..15860
                     /locus_tag="ACGI68_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137393.1"
                     /translation="MDNLERNIQLIFSYLAKTFIRNGASIKDAEKEANEIIKANSNNL
                     WGVNGLAYQLGKINLEFFCMYFMQDTYLPKEDNAAAPIAKVHHELWEDIQESIIGDGS
                     QQLGRIYPRGTGKSAFGDLATTVWSHCYKHKTYTLICSDIGSTAEKFVKDIKNALLEN
                     EYIKKAFGVLLNDNDRKYICNSTQLELTNKTFIEAISSSSPMRGRKYNNNRPDLIILD
                     DYQSEENVRTEDAREKKFKRFSDDVKYAAQKPVIRNGKTIKRGTTFIALGTLQHKECF
                     YSRLKNLPTWKFKCEKGVLVDNVDELFNSGLWLKFKEILFDFKNTNHLEDAKEFYWEN
                     EKEMKFPILWPSFWDCLDMALSYYENPTSFKQEVQNDVNSIGEKWFKTVRTETREEIE
                     SHTFKKTMLLCDPASAGGSKHDYSAFLVGSESENGLLYGRLAELAKINARTDFDKYID
                     HMIYLLKVYSDITHIYIEKNTFNGADANQLEFKIKNDNVLSYRDIEIINEQQKKNKDD
                     KISTLIPVLSKGQMIFAEEDKEFIQQILDFTGQKYSLHDDAPDISSEFINRINNIKVS
                     ETVNIFDRKLLGF"
     gene            15876..16064
                     /locus_tag="ACGI68_000946"
     CDS             15876..16064
                     /locus_tag="ACGI68_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137394.1"
                     /translation="MANRSKFEEVQKTLTKKGKKFNVGIGKDEKGYFAYTHRARSKSY
                     ISKQDIPIKVLKFIESTG"
     gene            16077..17354
                     /locus_tag="ACGI68_000947"
     CDS             16077..17354
                     /locus_tag="ACGI68_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_process: GO:0099001 - symbiont genome ejection through
                     host cell envelope, long flexible tail mechanism [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0137395.1"
                     /translation="MELDLDLIKDIYDDWNSNKSEYDTMYKYYKGETDAISNYKMVTK
                     RSNNKINTNFLKKFINEEVAYSLANKITYTSRSGDEKIINDLEYYTCHWSKKHDSDLL
                     RYALLFGLCYELYYVKDDEIQVKIIKPTDGCHYENENGEIVCFIREFTKGFEDDTYVD
                     VYDKEYIYHFDSNFKEVESPTVNNIFDGNVPISICKRSEELGKNTIFNDIKGLQDAYE
                     TNLSDISNEISDFRNAYLTFSGCNIKEDDLPRMKELGILQVNGDGKIEWLIKDMNDTF
                     VQNTLSSIKENMYEITSHINHNEKMQSNTSSLAVIARLISTEWICSQNNDSIADTLFN
                     RYKLLCIWLNKKYGFDYDYKDIKAKFTPKIPRDDLVVANILSQLGDKLSTETGLSQLS
                     FIDNPHAEIEKAKKEQEIVSEGEILLDESKDYN"
     gene            17332..18093
                     /locus_tag="ACGI68_000948"
     CDS             17332..18093
                     /locus_tag="ACGI68_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFH0137396.1"
                     /translation="MNQKIITKLTEDIYKSAENRTKPFYKYQRENRDKLLLEIAKILL
                     THDIINEYLNINNKDKKNLRSKLNKIINEYSNSYDEEVKDIETILSDSSKNKYSNLII
                     LLGVILKIKDVSNINNKVINKIVNNKVDGKHWSDRLWKNKKGIEESLKKEIKSFLNGK
                     TSVNKIEKAIRDKYSTGAGQTSRLIENEVARCQSEVNEYFFKVQGIIKVMYCADLDSR
                     TCSDCSMHNGVIFYVNEDRPSLPRHCHCRCQYIPV"
     gene            18152..18478
                     /locus_tag="ACGI68_000949"
     CDS             18152..18478
                     /locus_tag="ACGI68_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137397.1"
                     /translation="MKVEILGTEYLVIEKLESEDVLLKERAGYCDHSVKEIVIEKINS
                     EEGSLKDLSVYKNEVARHEIIHAFLSESGLKSCSSWATNEEMIDFFAIQFPKIVKVMD
                     KIECLN"
     gene            18504..19106
                     /locus_tag="ACGI68_000950"
     CDS             18504..19106
                     /locus_tag="ACGI68_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFH0137398.1"
                     /translation="MLKKEVLELLKEIEDDAEIDSLLQSTDLFKSASDKKLTIEEFRE
                     LINNDANFKAIIDNEKNKYHSEALENFKKKDMQTLISAEVLKRTGANETEEQKAIREL
                     RESLDKLKKEKQYAEKVSKYKDILVEKKIPTNLIEYLLSDDDDKTNANIEIFENSMKQ
                     YVQSKVDERIKDGSYVPPGKDSAGSLSEIRKQIKQGLNSL"
     gene            19135..20058
                     /locus_tag="ACGI68_000951"
     CDS             19135..20058
                     /locus_tag="ACGI68_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137399.1"
                     /translation="MANVLQYVTLFQQELDMQVTVGSTTGWMEKNAGQVIYNGGREVK
                     VPKVTMDGLGDYDRSKGYTQGAVTLEYETMKMEMDRGRTFMLDSMDVNESNFVINASS
                     VMGLFQKQHVIPEIDAYRYSKIFSETAKVDERVKYSYEATVTTILSELKNQLASIQDL
                     SGDIPLIISMSSIIASILEQSSQLDDIEFGDGDIKRIVKSIDNVPIIKVPSKRMKTEY
                     VFRDGKTSGQTEGGFTEGAKALDINWLITPASAPIAVSKTDKPRIFTPDQNQTADAYQ
                     IDYRKYHDLWILDEYLKLCRASVRQAKAAQE"
     gene            20072..20344
                     /locus_tag="ACGI68_000952"
     CDS             20072..20344
                     /locus_tag="ACGI68_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFH0137400.1"
                     /translation="MFALIKNNIIKVVEDENSRDKLISKGFKIYSEEVTKENIEKKEE
                     KVKSTSNDLFNLSKPELMEIAKGKNIKGYSTKTKEELIQLLDEYNA"
     gene            20328..20708
                     /locus_tag="ACGI68_000953"
     CDS             20328..20708
                     /locus_tag="ACGI68_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFH0137401.1"
                     /translation="MSTMLDNVKLVLGIENDRYDKLIILYINKISEMVLEYCSRQELS
                     TALEGFVEEKVISILSSRFEELKKNSGLDNLEKSLKAITRGDTKIEYNALTLQTAENS
                     ISSSSFLTDTDKKFLSPFCIVSMY"
     gene            20720..21067
                     /locus_tag="ACGI68_000954"
     CDS             20720..21067
                     /locus_tag="ACGI68_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137402.1"
                     /translation="MTEADILEMTYLDKMTIIRKLKGWNEETCSNDFSNVVIAENIPC
                     AISRKEEAIVNGDIGSIVVNRKLFCRPEVDIKIGDTINTTYNHGEEAVFKVSKANIYP
                     SHMEIPITELERI"
     gene            21067..21423
                     /locus_tag="ACGI68_000955"
     CDS             21067..21423
                     /locus_tag="ACGI68_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFH0137403.1"
                     /translation="MGIDNLGVFEALALDLGLNASNFNRKIKKFTKETGQELAENIRD
                     ETPVKTEKLKKGWEEKQPEYDTYIVKNEVDYATLVNNGHSTKNGGIVPGSFMVEKGIE
                     RTKENMTSAKANLFDW"
     gene            21432..21881
                     /locus_tag="ACGI68_000956"
     CDS             21432..21881
                     /locus_tag="ACGI68_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0137404.1"
                     /translation="MLTNRDIIIAVNKRIENEFRYIDIFINEDNIQGFEEACFFVQLL
                     PVSNNIANKVLDTKKIFIDIEYYQKARKNKLNLYDIQSRLEKIFNRNIRVKDRSLIIE
                     NISPSIAKDPVGYKLTFLMTVSYFEEVYFENETFEIMESVDLEIGSD"
     gene            21882..22937
                     /locus_tag="ACGI68_000957"
     CDS             21882..22937
                     /locus_tag="ACGI68_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFH0137405.1"
                     /translation="MGLPEINISFIQAGKTFVKRSGGIVALILKDTKNIGLVEIKEIE
                     DIPENYSAANLDYIKMAMKGNVYPPNKVLVYTLDTDESIDDALDFLETCEFNYLCMPD
                     ETEQDLPKIKTWIKKMREDNKIKVKAITASNSADYEGIINFTATDIEVEGKKYTSNEF
                     LPRIAGFIAGTPSTQSVTYAEIPEVTNIPKLTRAEANTRINNGELILIKESGAIVIAR
                     GVTSFTTITDSKGDIFKKIKLVDTLDQIHNDIKKIIVKNYIGKTQNTYDNKCLLIVAI
                     QLYLQELEKDGLIDEGSTVEINLDAQRAWLKKQNIDISNMEEQQIKEYNTDTLVFLKA
                     RIKLIDAMEDVYLDISM"
     gene            22953..23393
                     /locus_tag="ACGI68_000958"
     CDS             22953..23393
                     /locus_tag="ACGI68_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0137406.1"
                     /translation="MKAKIDAKRIISGTFGECWLDNKQLTSTKGMEAKVDIDKEEIKL
                     CGQIMTTYKMIGATGKGSITLYKISTLFGEYVEKMLNEGKEFKVTIISKLQDPDSYGS
                     ERIALYGCMFDDLTLADWEAAKVGEIEVPFTFEGFEYLDKIKEK"
     gene            23408..23824
                     /locus_tag="ACGI68_000959"
     CDS             23408..23824
                     /locus_tag="ACGI68_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0137407.1"
                     /translation="MNTVEKLLNMDAGKLKMPSSAFELYCKKLDDTLEIECKAIDPER
                     FDEIRMNSMDVNSGDVENINIYELKVNTILESCSIFSNMELVKKFSAITPKELIKKLL
                     LSGEIDKLYEEVNKVNGIDAEGDKKRQKERAKDIKN"
     gene            23851..24000
                     /locus_tag="ACGI68_000960"
     CDS             23851..24000
                     /locus_tag="ACGI68_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137408.1"
                     /translation="MYLLFKYKGIMPSVSYHILRGEKEIVSVFMDQELDERKEEIKSG
                     VGRLF"
     gene            24004..27393
                     /locus_tag="ACGI68_000961"
     CDS             24004..27393
                     /locus_tag="ACGI68_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFH0137409.1"
                     /translation="MSSNTKVLQAIIKMRDEASSTLKRVAANTRALHDSSSQASRALN
                     NVQSNLKSVGTSALKAGGLITAGVGGAFAAATKVGTEFESAMSQVKATFGNSLKPGDF
                     EALSKAARDAGKATTKTAADSANALQYMGLAGWDVQKSMHALMPVLNLSEAASMDLGL
                     ASDLVTDSMSSMCLEVDKVSKSGKVLAGAGLSEYLDKVANASTKSNTSIQQLMEAFNV
                     AGGTFSNLKVPLSEATAIMGILANRGVKGTDAGHALNSIMVNLTTGAGQAGKAMKKLG
                     VSAFDNQGKFKGMSNVLKEVANKTKGMTDEQKNYYLSAIGGKTQLKTLQKLLDGVGNE
                     YDSLKKKIDNSKGALKTMSDVMKDNTKGGLATLKSALEETGITIYYQLKPAIASVVDN
                     LTKMTVWFNNLSEPIKRVITNVMLSVGAFGVLLLTIGAVSLIVSKAIGIFGFFVPIIL
                     RAKVAFRAFVIGMKPLSAVFLAVMGPVGWTITAVIALSVAFTIAYAKSEVFRNKVNSL
                     VKSFTEFAKPIVNFCMPALKQLGEKFKELLSAFGPLFASILNLGSVIMKVLGPVLIGV
                     IGFHIAKLVLILSTLATAFKAVVDTITNVLNGLASILNGVFDLIIGIVTGDSKKMMDG
                     VKQIFDGGTKIIKSIWKGFIDFVTAPIQAIADIADSLFKDKAKWLTDKWRDLRTFLEN
                     PIEATAKISTKTNVNIPGSKAADAAGKSTPNTGGLKQTVANLKGSFSDFKTFIKPMTD
                     SISQGVESGKTKFTELQNKILNDVKPALKVLEDSGVNFKDKFKESITSIKDKFNELKN
                     ILAGNIKTNIDNLVKAFEPLKPHLDNLKDCFDKVKKAITDFFTPAKQVGDTVNKVSQP
                     MDSVKKSTDGIKLSFQSLTQALAPVKAWFQGFMPFLSQIGQQLLPSIGIAIAGVVGGI
                     ILAFTWVLNTLTAIITTVSGIINGIMSFISGIINVIMGLITGNADQTMDGLKQIFDSG
                     VGVIKSIWNGLVDLVSGVLEPVIDLADSLFKDKVKGVKEAWNKLKDFFKHPIKGTIEI
                     FKKGPSLQIGKGADAAGKSPRKAFGINRVPRNDYLIRAHEGEKLLTKQEANQYKRNSS
                     NGSLFLDKLADTIIVREEADIDKIVRKINKKISLAKIGGVL"
     gene            27440..27916
                     /locus_tag="ACGI68_000962"
     CDS             27440..27916
                     /locus_tag="ACGI68_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HFH0137410.1"
                     /translation="MNKKILVLTISIILSLSLVGCSTNNSKNRDSKSKQNTEEVKNHK
                     IGEVVDLDGAKVSVKNIRKLETNSNDYAVEIEITNTGDNDLQSDYLENYILKSSDNTT
                     GEAINNQELNGELMFDTIRPGDTLKGEIAFELTKDATPKTLEIAYENVGKYTVFDL"
     gene            27974..28609
                     /locus_tag="ACGI68_000963"
     CDS             27974..28609
                     /locus_tag="ACGI68_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFH0137411.1"
                     /translation="MEIWLRQANNTFRFPVIPPSFEINGSATINTSNILSVGDIAVFG
                     GLGLKTIELSSFFPNQEYSFCNYNGFPKPYDCVNLIESWMKEGYILRFIITETNINFE
                     CIIADFNYREQDYSRDIYFTLSLKEYRRIQISKVNINNDEKLSSEKNVPLTKGFDTKQ
                     KTHKVVEGDTLFKIAKKYYSNGDLWEKIYKANEDEIKDPSVIKNGWVLIIP"
     gene            28850..30367
                     /locus_tag="ACGI68_000964"
     CDS             28850..30367
                     /locus_tag="ACGI68_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0137412.1"
                     /translation="MIIDRSKDSSNNNIDFTAKDMGFLLLQSEVSYNFKDKLVEDIAK
                     QVFVDNKFPLGNLPKTNVKYTKMFIGVTGYDTIMSVYTEASKTTKKKYMIESNLDKFN
                     VIEKGVVTLNITFEEGFNLINTNFSESIENVKNKVLVVDQYGNKISEKINDSIFKDVG
                     VIMQKVIQQQENSTIDIDGEFKGIEKTCSLKGYGDITCVTGRGVKVKDSYTKLIGLFY
                     IDTDKHTWQNGDYQIELELNFENIMDEKSAGQDEQKEDGSEYTGGTEYPAEFTAYCPR
                     KEEGGNTDCRGKKLDPSKKTCAAPMVGAYEKSYYTKDFLNKHPLMRYGDEIQLVTGVS
                     SRDGTYKVNDNGPAIIIEKDGTYHIDILFGNVEEANNFGRRKGKIIIGGYSGNMTEKA
                     KTVISEARKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVNLPRTSSQQSKVGKKVEK
                     NNLQAGDLVFFHNPVSHVGLYVGNGEFLHAPQTGDVVKISKLSNRKDFNTARRVL"
     gene            30381..30713
                     /locus_tag="ACGI68_000965"
     CDS             30381..30713
                     /locus_tag="ACGI68_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HFH0137413.1"
                     /translation="MADPINEFIGIIRQEGKFHNEPSFFIGKIKSKLPDLKIEINNII
                     LEKEDILIDSWMLDRQIELFDTETSQEHKHEIKNPFIDTFESGNTVIMFKIGEKFAVV
                     SKLVSLDE"
     gene            30706..31140
                     /locus_tag="ACGI68_000966"
     CDS             30706..31140
                     /locus_tag="ACGI68_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0137414.1"
                     /translation="MNNTIFPFMGVPEDYISPDNEELPIFKEFAWDFNKDEKIIENGD
                     FKIVERNEAIKVWIYKTIKTDKYVHLIYDWSYGTDIKNLIGQKYTKGLTESEAKRYIQ
                     EALLTNPYILEVNITNAEFEDDNLSISLNVKTIYGEEEITFV"
     gene            31133..32185
                     /locus_tag="ACGI68_000967"
     CDS             31133..32185
                     /locus_tag="ACGI68_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0137415.1"
                     /translation="MYNGQSFSTLNNRTLNNINLPLYKGQGSSLYNIVSPINSELAQL
                     YIELSYIHKRVFIQDNFDDFLDRRVNEFGVYRKLGTEAIGEVTFEGKIGTQIPNGTII
                     SHSDLLFVIIKDITIDENSKLNVSPIQALEIGIKYNLSANTEFKLIEEITGVTKIYNE
                     LDLKGGTEIETDEELKERFYKIQKNQATSGNKAHYQSWALEVEGVYNAKVIPRWDGPG
                     TIKILIYGQNNQSVDNEVLQRCTEHIEEEKPIGPTVTIVTPSTFDINISATLTLENGY
                     DIESIKVVFLDIINSYLIENSREIVYIKVMSLLASIEGIHDIKNLLVNGDVKNIIVDE
                     EKVPAVSSAIFDIEVS"
     gene            32187..32795
                     /locus_tag="ACGI68_000968"
     CDS             32187..32795
                     /locus_tag="ACGI68_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFH0137416.1"
                     /translation="MKLIDYLPSFANNEIDIQIQEALENELLTLIDEKDDLLEQFFID
                     TATWGLDDWENLLSIKVNYKLDFDTRRSNIKAKMRGKGTTTIEVIKAISEAYTKTNVD
                     VEVFSNLFSFTLSFITNNCSYNTILELDKKIEEIKPAHLEHKFERILFNKNELYTGAA
                     ISTGETVTIYPYVPRNLESFGEIAICSGNDRALEKVTLYPKK"
     gene            32812..33594
                     /locus_tag="ACGI68_000969"
     CDS             32812..33594
                     /locus_tag="ACGI68_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0137417.1"
                     /translation="MTEQQYFTLVTDIGKAAIANASITGEKVDFAKIKVGDGGGSSYT
                     PNESQTALKNVVWESTLEHAQGDKDNPNWVVIQKFIPGDVGGFEIREVGLFDSKDQLL
                     AISSYPTTYKPTADSGTVKELLIKVILVVSNVANINLKVDPTVILATLKDIQELDTKI
                     DTTKTELTSNIETAKTELNTKIGDTTQLTTTDKTNIVSALNEVKTSVDSIETTADKTS
                     IKDTDNLFESDNVEGALKEVMQEVKGNRSSIISTINSNLIPM"
     gene            33611..35056
                     /locus_tag="ACGI68_000970"
     CDS             33611..35056
                     /locus_tag="ACGI68_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137418.1"
                     /translation="MKIWKYNRTVKKRRGVYTELDILAQRYEFEADGKLYTAYFHLYT
                     NKNIDIFRDKDLYKESYMMSYRVGNVTTDIDDVVQQTTTVRFIPDPVIDIVIDNISRG
                     CEINYRIIDNEPSVKFIVTEKLNGTIIETKTNSSDNNYKLKLTDEQIVPLAFNSQNSI
                     VIEISTEDGGLVTSKTVTFTRTNNKPTILVNSYSSNSAKFTASDLDNNLSKIEWYLDD
                     VLKETITTDLTAEKTINYEFTDNAIHTLKIVATDAENATVEKVLSISKEIMPLQSDAS
                     LSDISTKLIEIGEGFRNGKTSIINTLALKNIEASLNNTLVELSEKIKTSFDSSDASVQ
                     DLQNRITELNNQLSRRKKWATGRYTFTREEDTINFRMVDGDSLSAVKKLIIPINLDFV
                     PSVIIIDKMFLGSYVPAQDVQFNCVSNLADFTLGRRFYNKQWICTSSIKLDSVTQNSF
                     TLSLQKVVPFDKVSSTIFSIINTGFAWRAYE"
     gene            35080..35427
                     /locus_tag="ACGI68_000971"
     CDS             35080..35427
                     /locus_tag="ACGI68_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137419.1"
                     /translation="MNRNNRIIYDQTGKIWLQTGEAIGDIQELSEITELNFIDVEFGS
                     IDYSKQYIESINPVTKELNIKNIEVVLTDEQKRLEVLEKELNMLKEENKNRDSEIVNT
                     AFEVGNIKLNNNL"
     gene            35439..35609
                     /locus_tag="ACGI68_000972"
     CDS             35439..35609
                     /locus_tag="ACGI68_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137420.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKMDVFYAVNRITEEQYLELTGL
                     LNKEETPVEPTI"
     gene            35639..36208
                     /locus_tag="ACGI68_000973"
     CDS             35639..36208
                     /locus_tag="ACGI68_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137421.1"
                     /translation="MTFKELVSKVRNLVLEAKNVTIEDTENKFTSENVEGALKECIDR
                     ADLAFQSADSGKELIATAIGSPATSEQTFQEYANYIAGFKNTISQLNSKYKYTSGTFT
                     YYTGAYPNGLNIGFRPDIISYTAINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNR
                     YGITFTDNGWIPRNPVNNGVYSYIAAKCR"
     gene            36267..36695
                     /locus_tag="ACGI68_000974"
     CDS             36267..36695
                     /locus_tag="ACGI68_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0137422.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGK"
     gene            36699..37529
                     /locus_tag="ACGI68_000975"
     CDS             36699..37529
                     /locus_tag="ACGI68_000975"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0137423.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADIFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDRQGKGSEVLYYSN
                     KGLEYATRICKKLGTVFKNRGAKLDKSLYILNSSKPTAILIESFFCDNKEDYEKAKKL
                     GYEGMAKLIVEGVLNKSIESNEVKQMYKHTIVYSGDDRVSADILGLYYKRAKESYLVI
                     DIKDYKPHRTQNLYVIGGVTCNKMKEMSKTTGEKFTQLYGNDVWSTMDKVIEFVKEKL
                     "
     gene            37601..38707
                     /locus_tag="ACGI68_000976"
     CDS             37601..38707
                     /locus_tag="ACGI68_000976"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFH0137424.1"
                     /translation="MLTFLDLFAGIGGFRLAMERAGHKCVGHCEYDKFANLSYKAMHN
                     PKEDEWFEKDIREIRAENIPKADVWCFGFPCQDISIAGNKLGFKGNRSSLFFSVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNRGFDFAKLLIELDEIGYNAEWQNLNSKNFRVPQN
                     RDRIYIVGHLRRRSTREVFPITGTSRKTTLEQLNNPNHSSNRIYNSNGLSKCLLSKGG
                     NNTGLYRVGNINPSGNGAGGQVYSSEGFSPTLTTGKGESPKILIKENTKKGYIKASIG
                     DGIRLDHIEGKTGRGRVQHKKSCTLTTASNAGVIGKKFRVRRLTPKECFRLQGFPDDY
                     YDRAASVCSETQLYKQAGNSVTVDVVEEIAKKLK"
     gene            38914..39180
                     /locus_tag="ACGI68_000977"
     CDS             38914..39180
                     /locus_tag="ACGI68_000977"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0137425.1"
                     /translation="MSKKEIIRICNEKHNWRKSATKTLLKRLVKKRVLLKRIKIFHIY
                     YIPIVTEKEYFKYKINDLEPDYLDEYFIRLMSTVHKFKNLDEED"
     gene            complement(39191..39706)
                     /locus_tag="ACGI68_000978"
     CDS             complement(39191..39706)
                     /locus_tag="ACGI68_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137426.1"
                     /translation="MTWSDLKEKNINEVVDCINNKLSEFESLKKVGDELGANESTIRK
                     WLNKKGYKRTGNKFVEDNKSYDDKYHIDSISSKIDDDIKYLSSEIDNIKNVLEWFKTK
                     DDSYHICVIKENDISIDLPDQSIKRTTVRINNTVWEMFNKFADENKHYDKHDLLSQLL
                     LESLLKYVKSR"
     gene            complement(39789..40694)
                     /locus_tag="ACGI68_000979"
     CDS             complement(39789..40694)
                     /locus_tag="ACGI68_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFH0137427.1"
                     /translation="MSKLGIDIGNYAVKTSTDDIFESKVTEVKNFGSDSDSIKIGNKT
                     YYLGEGDEEINIVKYEKENFLPLLLGAICRNTDDEIVDLGLGLPVKQFAGLRKNLIEK
                     LQGKEYHVEFTRGNETTKRDITIRSIQTFPEGVTGYLYYAKDIVDQIAGRDVVLVDIG
                     GKTTDIALVQGNKATDPYSINVGTINIYDAIKKSLEMDERFLGKVEIKREKIQDYINK
                     GFYLNGEKQDIKKNIDASISLFKEIYNELKLNYPVSTAAVVVMGGGAKLLGEAFKKNI
                     PGIIVMSDVDKHVFANAKGYKKMMK"
     gene            complement(40714..40917)
                     /locus_tag="ACGI68_000980"
     CDS             complement(40714..40917)
                     /locus_tag="ACGI68_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137428.1"
                     /translation="MSRIVLTFKNNDKEKAIEKFLDEKLSATAYLKELVWEKMQEKKD
                     NAVVEQKKEIEDPANNFDFGSLE"
     gene            complement(41166..41624)
                     /locus_tag="ACGI68_000981"
     CDS             complement(41166..41624)
                     /locus_tag="ACGI68_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137429.1"
                     /translation="MKKTIALLLTLCAVMIPFTAFADGNDYEGTGNSQTVELIGQPNS
                     DSPSTKSVVVPGGTANLTAMSGGQLSWRVTSTLGNILGFEGNIDVYKAGKIVSSQYVG
                     FRLAGTTKTGVVTFKGLKKGSYIAKFKGKGVCTGGIILFSSPAEASFTIR"
     gene            complement(41659..41961)
                     /locus_tag="ACGI68_000982"
     CDS             complement(41659..41961)
                     /locus_tag="ACGI68_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137430.1"
                     /translation="MEVNLHTGKDEFFKSHEVAKNYIGGLYKLIDVYKFSMESVSVLT
                     RIDLDKLNNFYAGEASLNYDELCLIESVIITPLVGAFETAEHNYNMIIKARKERKL"
     gene            complement(42349..42501)
                     /locus_tag="ACGI68_000983"
     CDS             complement(42349..42501)
                     /locus_tag="ACGI68_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137431.1"
                     /translation="MDNFLFNVLASLTASVVVYLISELFKKAKSHSRAKSDLQVEFKF
                     IFKFKK"
     gene            complement(42621..43355)
                     /locus_tag="ACGI68_000984"
     CDS             complement(42621..43355)
                     /locus_tag="ACGI68_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0137432.1"
                     /translation="MYYVYKYINLDTEECLYVGKTENICDRHASHLSNKKENWCNKNL
                     RFEYIELDNKYTMDFYEIYLINKLEPKFNITGKGEMDIAKTSFSYNGQWVIYSERDLM
                     SSLASKRCGINYEVNAKMLGIMRKLKKISSNEEIFIGNENIRIKYYFETVLEGINLEP
                     CILSVAYKTLKESTVGTVISVKREYLSENVCLIIDSIFLNEIMKDPLMSKSDIKDLNS
                     QVNDILKIIGVDCEWNKLSDYCSANS"
     gene            complement(43375..44562)
                     /locus_tag="ACGI68_000985"
     CDS             complement(43375..44562)
                     /locus_tag="ACGI68_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0137433.1"
                     /translation="MEKKEILMQPNNLIKSKYDFTNVENKLFYKILFNAQKQQNSSYV
                     TTISKEELKVFMKNNNDYEHKNIKEILNMFQQSVLEFDYIEETTGKLKTFGSGLINTY
                     ELDHTDQIYTIMMHEVLYNHITDFVKMQKKKNGYTAINLSVLFNFRGAYTQRLYTLFR
                     LWSRENKEVEIKYKLDELRFYLKLKDNVYPEYKYFKQNVLKRAMNEINKKGNMVVSIK
                     EEKRKNRKIDEIIFSVIDYEPRKYFDKDILVEDYIPKKKQDNEVSFSFYIPNENIFTT
                     GTIKLFKEDFKEFDFKKDVYLNAFNKSIAIAFERDNTDIINTKNYDFFKSTLSNKISE
                     VIIHQKDDLKFKEELDKYWDDEDVKKEEREVDYSKDPVRLETVRRNLFDQGLVNEEGY
                     YYK"
     gene            44982..45671
                     /locus_tag="ACGI68_000986"
     CDS             44982..45671
                     /locus_tag="ACGI68_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFH0137434.1"
                     /translation="MLENRKQIQKEIYLPIATYCLKDKQYYIKEYGALLIKSAENSDI
                     FNTHKNRILYKNKLDIQEIANELNVNKATIQRNIKKLEKLDFKILKIENTLNGIVYCL
                     PSENNIDLNKFALINYEMLKKMVNKFKSNTIKVYFLLKTITTETNFKPATNSFIAENI
                     GLSSKSKNNLDIITSSVKTLEENKYIETKKVNVYEYDKEKLREVPKIRKVYRICNFDE
                     WKKEIDKNKVY"
     gene            complement(45820..46203)
                     /locus_tag="ACGI68_000987"
     CDS             complement(45820..46203)
                     /locus_tag="ACGI68_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0137435.1"
                     /translation="MKIKKIPESELLVMMFIWDSEDKEVASTEILKTLGEKYEWKKST
                     MLTFLRRLVGRGFLEVVKKDRFTYYKALIEKEEYLKVETKSFFSFFHKNSFESFISAL
                     HDDEEVSENKLKSLEKFIEDWEEDK"
BASE COUNT        18599 a         5392 c         7720 g        14604 t
ORIGIN      
        1 aaaaaaaaat taagcaggag taaaaaaaat aaaatattat caaaatttat caatataaaa
       61 agaaatataa gtataaattt ttctctttgt atattgattg tatacaattc tatttttgta
      121 aaattaaatt ttaaaaaata atcaaaaatg gtaaattttg acatttgctt aatgtcaaat
      181 taggtgttac aattgtagca taaagaagat atatatcgaa ataatacata taaaactacg
      241 tacgtaaaat atttttaaaa ataatcaaaa atcttaattt tcaatattga atacaattaa
      301 attttcaaac tcatctaggg catgacatac aaaagcaaaa aacattaatt tagtatctga
      361 taaaaattta aaaattgtaa aagggggaag tctgttattt ttcagaagca atgtaattgt
      421 aaaaatataa atcagaatat tttgaaaata tgggggataa acttatgatg gatttagaaa
      481 aggaaaaaca tgaaataatt gaaattataa aaaaattaaa tattgataaa gataaaagga
      541 atttattgat gataaaaaat tatatccttg gacttttaaa aaaatagaag aatagcaatt
      601 gctattcttc tatttctagt aaggaatcta tcattttatc aaatacagtc agatattttt
      661 tattcaattt gaacatttta gttataattt ctctttctac ttcattttcc attttactta
      721 gttcaccaac aagatgggca aattgctcat ctaattcttt gataaaaaac atctctccaa
      781 ctccatgctt aagccattct tcagatatgc tccaagtctt gcaaatgtcg ttcaaagttc
      841 tttcgcttgc atttctttta ccattttcaa ttaaagatat tgtgtttctt tttaaattta
      901 gtttattagc aaactcttct tggcttaaat tgagatttaa tcttagttct cttattcttt
      961 tgttttctgg tttcatttca tctatcatta ttatccctcc atatgaaata ttatatttta
     1021 aaagtgctta caagtcaaca aaaataaaag aaaaaggtat tacaaaatag ttgacttgta
     1081 agcagacatg taatataatt gtattacaca atcaacaaaa gcgaggtgat tgaaaatgac
     1141 tgatataaat aaaattagag aagaaatata tagaaaattg gagttacttg gccctgcaaa
     1201 actaaaatta gcattgacta aagtagatat attaaaagaa atacaagaca ttgaagatga
     1261 aaataatata caaaattaaa ggggtgaaaa gattgaaaat ctcagatttt aataaaaatc
     1321 aaataggatc agcagttcaa ttacaagata taaggtttga tacaaaagct gattgcatca
     1381 ttacgaaagt agaaaagaat gaaatactag tcatgtatta tgaaaaagaa acagaggaaa
     1441 tagcatataa aactttgact aaagaagatc taatacttga tgactataaa cttaagttat
     1501 tatcttaaag taatttcaga agggaggtga taactttgga tcttttgaag gatgcaagaa
     1561 aaaaagagtg gttttggctg gaaaatgatt tgattgatag agaagatttg agcatctatg
     1621 aaaaaatgat ttacattgtc ttagctagat atagtaacga agaaagttct tgtttcccaa
     1681 gttataaaac aatatcagaa aaagcaggtt gcagtataag gcaagtatct aatattttga
     1741 atgaattaga gaataaggaa ctaataataa gagaaaatcg taaccacgaa ggcaaaaaag
     1801 agaaaaacag taacttatat ttcttaatat cttcaaaagc aaaggtacag aatgaagtgc
     1861 ctaacgttag gcatgaggtg cctaacccta tggcaccagg tgccttacat gttaggcatg
     1921 aggtgcctag taaaaagact tataataaaa agacttatat aaaaagtaat accactacac
     1981 aaaaagaatc taaaactgat tatttagacc tatcattctt ggacttagat atagaaaaag
     2041 taaaactaac taaagatgaa tatgacaaac ttataagcaa gtttgggaag aaatacatac
     2101 acgataaaat tgttagttta gaaaactata tcataaatgg taaagggagt aggtataaga
     2161 gccactatag ggctttgctg acttggggga atggagatgc tagtaaaggt atagtaccag
     2221 aagttacaaa gactaagaat cctcttagtg gatttaaaga actttaaaaa ctaaatagta
     2281 aggaggtaga taggatgaat gaaaaagaag agactgttta tttgaatagt tctaatgtga
     2341 agttattctt aggcgttagg gatattaagg aatttgagga attgatagaa aatgtcaatg
     2401 agcgaatttg tcaactccaa gaatcgatta ataaacttgc ccaatttaat attgagttcg
     2461 atattaagtc agatatttag ttaagtataa attaggaggt tgcctatgaa agatgaaaag
     2521 ttagaactta ctgaaaaaga tttacactgt attgcaagac atttacaaaa tgaagttatg
     2581 gagatggtgt ttagagggaa tatagaagcc cctacatcat gcgaagtttg cgattacttg
     2641 caagaatgca aggactattt cacccatata gacactttta taaaattaag tgaaatgaca
     2701 ggtgtagata tttttactaa ataaatttag taaattaaaa ttttagtatt gtaattgacg
     2761 tgctttttta aaaagaggac agttgctatt agcagtcatt atttgaattt tgcaaatgta
     2821 atttttatta cattcgcatt ctaaaagttt tgatccagtg aatccaggaa aagttttata
     2881 ttcaccagaa aattcaactt tatcgtcaat aagagagcat ttttctctta aggttttaat
     2941 tttgcacata ttcatcacca ccctataatt aatttgaaca tactgtcaat atgctccgat
     3001 taattataac atgcaaatta tcatgatgta gaattaaagc ataggtcaat taaatttagt
     3061 tataaaatca atgaaacaga taaataatta aatgttaaat aaattagggg gttgttcatg
     3121 aaagaggaaa agttagaact tactgaaaaa gaattgcatt gtattgcaag acatttgcaa
     3181 aatgaagtta tggagatggt gtttagaggg aatatagaag cccccacatc atgtgaagtt
     3241 tgcgattacc tgcaagaatg tgagggagat tttacctgta tgtatcattc ttttactaaa
     3301 ttaagcaaaa taacaggtgt aaaagtgact gcatttaagt attgttttcc agatgaaaat
     3361 cccaaagcta ctatctaaga agttttttta gaataggaca ttcttcatta gaagaagagt
     3421 cgcaaatatg acttccatca catctagttg acattaactt agatccaaaa gtacctggag
     3481 gagctgaata ttcgcaagta agagaaatgg tttcgtttat tacaggacat tctttgctta
     3541 taactcgata ttggctcata ctctcaccac ctttctaatt aatttaaaca tattggaagt
     3601 atgtttaaat taattatagc atgtaaatta tcatggtgta gaatagtttc aaatcaaaag
     3661 aagcaactaa atagttaaag acaggaggta tgctaagtgg aaagaaaaaa acatgaattt
     3721 acagatcaag atatacaaag acttattgaa ggattaagaa ctgatccaaa aataagagaa
     3781 atgatattta aaagtaaagg ttgtgaaact agaacaagta caagaagtaa atgcgaacaa
     3841 tttgttgaag aaaaacttgc ttatagaagt tatggatttg aaactccaaa atactatgca
     3901 aaacttattg ccgacataat tgatatttta gtttacaaaa atgttccaag agatcgtatt
     3961 tgtggggtat tgtatgacgt cgaaagggtg attcctttag ttgtgacatt ttaataataa
     4021 gctgggggtg attaaatgaa ttactcttta gaagcagagc aaaacattct aggaagtttt
     4081 cttataaatg actctatagg ttacaaaata agggaattaa aagaaaatga tttctacttt
     4141 gagtcacata aaattatttt atcttgcatg aagaaaataa ttgactcaaa taaaccacta
     4201 gatcttttgc ttttaaaaaa tgaattagaa aaaatagata gattagtaga tattggaggc
     4261 gttagttaca ttaccagttt aacaactata gttataacta cttcaaacat agatcactat
     4321 ataaaaatca taaaagagaa gttacttaaa agacaaattt cagagttagc aaatgaatta
     4381 attaataatt caaattcaga tacaagtata gatgaattga ttatagatat aaacgattta
     4441 aaagcacttg tgacgacgag cagtagtgta aatgataatt acattgacgc atcaaaaata
     4501 aaacgtgaaa aaggcgtaca caagtccata gacacgggtt ttaataaact aaacaatatg
     4561 ctagatggat ttagatatgg aactcttaca attttaactg gtaaacctgc atctggtaaa
     4621 tcaactatag ttaaccagtt tatagcacaa gcaattacga atggagagaa agcatttttg
     4681 tattctggag aattaccctc tttcatgtct atggattggt ttagaaaaac tgtagctaat
     4741 gattatcaca taaaagaata taaaagtgtc tatggaggaa catacacaga tattccagat
     4801 tacgccgttg agcttatatc agactggata gaagataaat tttttctcta tgatgaagat
     4861 gcaataagtg atgaagtaaa tctactaaat accatagagc atttatattt aaaaaaaggg
     4921 gttaggttct ttgttcttga caatctaatg acaataaaaa ctggcaataa ggcagataaa
     4981 tatgagagac aagagcagat agtaagcaac ttaaaaaatc tcgctaaaaa atataactta
     5041 gtaatagttt tagttgcaca tcctcgtaaa aatatgggtg acatgaagcc aactatgtat
     5101 gatgtgtctg gagcaagtga gattgttaat tatgcagatt atattttatc aacttacaga
     5161 gttgttgatg aagaagaaga gacagacgac acttatttat taattttaaa aaatagaata
     5221 acaggaaaac agaatataag ttttaaaatg aatttttcag aaagaagaaa aagactttac
     5281 acgactagtg aggagttaaa cagagattac aaatatgatg taaataaaca atatgttcaa
     5341 gtagaaattc cagaagatgt attttaaaaa aagtataaaa aataggaaat tttattaagt
     5401 aatgatttta taagaaaaac caatattgaa taatttaaaa attcaatatt gaaaaaaatc
     5461 atttttcaat attgaaaaac ggattttgaa aaagtattga atttcaatac taaaaaaagt
     5521 ttgaaaatat agcatttaca aggtttatat aaattaattt cctgacaatg tacacacagg
     5581 tttagatatg ctagcaaaaa aacttaaaag tagtgtaagg ataaaaaggc tatataaatt
     5641 tataaaaaga tttaaaagtg ttattaaatt agagctaaag tttattaatt aatttcctcg
     5701 tagaaaaata ttattttaaa taaataaagg agtgatttta catgatagca ttagaaaaat
     5761 ttgcaggtgg agttctaaaa gaaaagttta atacagagtt acaaaaagtt ttagataaca
     5821 ttgcagatcc aaatacagat tttaaaaaaa caagaaaaat tagtttagag atagtgttta
     5881 aagcaaatga agatagagat cttgctgaag tagatataaa atccaaagca actatagtag
     5941 aagcaaaggc tacaacaact aaagttctga taggaaaaga tttagagaca ggaagagttg
     6001 aagcgtctga atttaaaaat caagtagcag ggcagttggt catggatatt ccagatagtg
     6061 aagaaactaa tgaaacagaa gaaaatagtg ctggtgtaat tgatttcaga aatgcaagtt
     6121 caaagtagtt taagaaagta agtaaaatat aaatttaaat aaaaaaggag aataaaaaaa
     6181 atgataagaa atgcaatgga atatttagtt aagttaggaa aaagagaaac agtatgtgta
     6241 gatgggttta agtacacaac agatgcatta gaaataataa aagaaccaaa agctaatgaa
     6301 ttagaaataa ctacattgag tggtttagta gattatataa aaagtggcat agatcataag
     6361 gcagatgagt tgttattaat acaagttcta actccaagaa aagtgctttt aaaatctcct
     6421 ttgagaaaaa atagagatag agagacatat attgaatgtg ttgcactact accagagaaa
     6481 agatttgaca gacctttaga cttagatagt tttaacataa tgctacaatc agcatttata
     6541 aagaataatg atagagatac acttttaaaa attgcaggaa atatccaaga atctacagta
     6601 aaaaatgtgg gagatgatgg agtttctcag tctgtaacaa taaaaacagg cgtagctagt
     6661 gtatcagaag caatagtccc aaacagagta aagttaattc cttatcgcac tttccaagag
     6721 atagagcaac cagaaagtga gtttattttc agaatgagtt ctggaccagc agcaggatta
     6781 tatgaagcag atggtggagc atggaaaaat caagcaatgt taaacattaa agcgtacttg
     6841 caagaagaat taaaagattt taaaaatgta aatataattg cttaattgaa aatctaagag
     6901 ggcttacaat aagtcctctt agtaaaaaag gaggggatta gatgaaaatg atttctcata
     6961 tgaacatttt agaaagagca gaatttacag ataaaatagc taatgcaatc atgtctgcaa
     7021 ataaagaaat tgaaagaggc tgtgccttta atgaagcagt tgagattgta aaacaaatgg
     7081 aatatagaga aaataaatac aatgaaggag atattgcaat atgaataatg tagttttagt
     7141 tggaagatta gcaagagatc cagaattaag atatatacca gagtatggta ctccagttgc
     7201 tacttttgca ttggcagttg ataggggtta tacaaaaaag gatggaacta gagaagtcga
     7261 ttttatacca attgaagtta tgggaggatc cgcagaattt tgtgctaatt atctcgcaaa
     7321 aggaagaatg gtttcaattc aaggccaaat aaggatagaa aaatatgaaa aagatggaga
     7381 gaaaaaaact ttcacaaaag taagaacaaa aattgttaat gcgcttgatc gcaagccaaa
     7441 ggatgaagaa aaagaaatta gttttcaagt tttagatgat gaagatattc cattttaaat
     7501 tggatggtga ttatgtgaga agtttagagg agagaaataa attagttgaa gaaaatataa
     7561 attttgtatg gtttattata agaagatatt tcaaagtatt tatttcaaaa tatccatatc
     7621 ttcaagatga tacattttca gaaggatgta ttggattatg taaagcagca gattattttg
     7681 atagtaaaaa aggtattgaa tttaccactt ttgcagctgt gtgtataaaa ggtcaaatat
     7741 ttaaatttat aagtaggtat gttaaaaaac attacagagc agatatagat agttttgaaa
     7801 aatgcatcta taaaaatact catggtgaag aaataagaat agaagataga ttggctggta
     7861 atgaaaatgt agatatagaa aatgttcatg ttattagatc ttgtataaaa agaagtaaaa
     7921 tagaagatat acagaaaata acatacttaa gggaaaaagg atacactcaa aaagaaatag
     7981 gcaaaatact taggactaac caaatgacaa tatgcaggaa gcttggaaaa ttaaaatctg
     8041 aaattaatat actaggaaaa taatataata atttaagttc attggaggca aaatgaagac
     8101 taaaataatt ggtggtaggg aaaatcagtg cccactttgt aatggtagtg tatttaagat
     8161 tgaaacatca attggaatag tatggcaatg taaggattgt ggatgtatgt atcaagatat
     8221 gaattgtaaa gaaaacaaaa ggaggcatta agtatggctt gtgttataaa gtgtgatttt
     8281 tgtggtgaaa caatagcaga aattctaaac ataaaggtat tcagaattga tgatgaaggt
     8341 aatataatga gtatcggaaa agatgtatgt gacaaatgtt atgacagatt atttagaagt
     8401 aacttgaata agaagatgac taattatgaa aagataaaaa atatgagcaa aatagaaatg
     8461 gctgaatttc ttgcaaatgg cggaagtgga tgtactagtt gtgcttatga ttttcaagaa
     8521 tgcttaggcg gatgtttaga aggtagaaaa aaatggcttg aaagtgaggc gatgaaataa
     8581 tgaaaaagac gttaataatc gtaggagtag ttatttcaat attaattatg tttgcttgct
     8641 tgttcttagt aactaaaaat aaagcaattg atttagaaga gcaggttcaa tcagcaactg
     8701 catctgtaga ggtgcaagaa aaaaggagaa ttgatttaat taagaacata gtagactgtg
     8761 taaaagacta tgctaaatat gaaaatagaa cattaaaaga agtaactgac atgagagtaa
     8821 gtgcaaatga tgaatctatc aaggaagcaa aattaaatat acaagcagta gcagagaagt
     8881 acccagattt aaaagctaat gaaaattata agcaactaat gagtgagttg agtataacag
     8941 aaaatatgat tgcacaacat agagaaaatt ataatatgca agttcgcaat tacaataaat
     9001 atataagaaa atttccagca aatttaattc taaatagtat gggatatgaa aaaatagaat
     9061 ctaaatatac tgaatataaa gcaaaagagg atgcacctca aaatttattt gattaagagg
     9121 tgcacatatg aaatttaaaa cttttgaaat aactaagaga gaatttttag tgagcataat
     9181 aattgcttgc atgatgattg tgcttggatt tttcattgat gataaagttc aatcaaaatt
     9241 tttagaagat aatgaaagat attacaaagc aactaaaata aataatgaca aagatctctt
     9301 tgattatgct atggaaacaa gtgcgggaga ttctttgata agtggaagat ttaaaagtgt
     9361 agattctgta tctatagaag aattaaaagg ggactattgg tttatagaaa gaacggaaga
     9421 aaaatacact atgcatacta gagtggttac cactaaagac agtaaaggcc attcacataa
     9481 atatactgaa acatattgga cttgggatta ccatgattca atagagaaaa aatgtaataa
     9541 atttaatttc atgggtaaag agtttgatgc gaaagtaatt actaatttat ctaaagagag
     9601 attggcatta aagaaagata tatttaaaaa taataatctt aaaataaaag gtaattatgc
     9661 ttacattaat aataagagaa ggtactatta tgaagttata ccgaaagaat ttaatggtac
     9721 tatgtatgct aatttaaaaa ataatactat agcgaatcca gctgataaaa acataaaagt
     9781 ttttaagggt gagtctatac agaatgtttt agaaaataat aattttaata cagggttttt
     9841 taaagtagtg ttttggatca tctggatatt aataactaca atcattatat atgtatttta
     9901 ttatttaaaa aatgattggc ttgaagataa ataaagatgt aagaggagaa gatcaggtat
     9961 gaaatgtaag gtgtaccatg ctattaaaaa tataaaagat gcaaatgagt taattgatag
    10021 atttgaaacg gaaaatatat tctttgaatt aaatgacata gataatgaaa atgtcttaaa
    10081 tggagaaatg aatttaactg atatagaaga tattgcatca ttgcaattta tattagatgg
    10141 agatttattt atatttagta gttgtgatga aatgatattt atgaataaat acactcaaaa
    10201 agagttggga cttcaataaa tgaatgaggg gagatactaa agatggattt aaagttaatt
    10261 agcagtatac ctaattggaa ggtagaagat cagttggcaa aaattgatga agaggtaact
    10321 gagtttaaaa atgctataga tacaggaata actaaagata taattgcaga aggattagat
    10381 gtttgtcaaa ctattttaac aatgtttcaa gttttagaaa tagaaaacta tattactgaa
    10441 ggagtagaga cacataataa gaaactaaat ggaagaggat gggatctcaa agaaatagga
    10501 gacgatatac ttaatttaat taaaaattca atgattaatg aattgattaa tatgaacaag
    10561 aaattaaaat gccttgatga aaaagaattt aatgcaaaaa tacaattttt agatgtagat
    10621 aaactatgga atttgtgcat gcctttagtt aacttaagta ctataaaagg agttaatgaa
    10681 agcattgatg aaatgaaaat gggagagttt ctaaaaaata ttaattaaag taaggagata
    10741 atataatatg ttgatagaag taactgaaaa taatagagaa ttaaaagtag gagatattgt
    10801 tgaaattaat ggtggtaata agttatatat gattctttct ttaaaggaaa caataggtgg
    10861 gtatgttatt ataaatatgc agaatggata tggttcattt ggtgcttata aaagtttaag
    10921 tcaactagaa ttagacttga aggtaagagg ttataaatta tatagttctg atgattacaa
    10981 gttgcaatta gtgcctagag aataagatat atttcaaaaa agaaaaaaag gagcattact
    11041 tcacgctcct gcttgtcaaa attctaaaac cgtcatcaca acatcattat aacataatta
    11101 ggagtgtgag gtaatgcaat ctagtaaaaa agacaatttg tttagtagtg cagaaggtaa
    11161 gttatatgat tataaaaaaa taaaagcgga aatagaaaaa atagacattg atatgcagat
    11221 gataaaaaac gactacagag gatgtagtgg agttgaaatt aaagaaaaga caggcaagac
    11281 atatgatatt aaaagtgtaa ttgaaataga gactgaagaa aaagaaaaga aaattgcctt
    11341 aaaagaaaaa gagaagagac ataaagagtt gataatagaa aaaatagata atgcaatgaa
    11401 aatattaagt gaagaagaaa aaaagatagt acagtataaa tacttttcaa gtagcaggac
    11461 atcttgggaa tatgttggca gaatgattgg tttttcagcg agtaagtgta agcaaatgag
    11521 gtttgatatt atagataaga taaaaggatt gctataaaaa tgtccgattt gtgacgattt
    11581 taaaccgatt tatgtctgtt ttgtgtctaa aaaatgtccg gtttgtgacc aatttatgtc
    11641 tgttttgtgt cttttaatca tgataagatt gtattgtgag aaaaatatat ttaataaaaa
    11701 tggcttggaa tttacgttct aagtcttttt tattgtctag agaggtgttg aagattaaac
    11761 gtgatataac agaagaaatt tcaagtgctg catatgtacc agacaatcta aagtattacg
    11821 acaatgtaat gaaagaaact cctggtatgt atggaagaga atgttcttta gattttataa
    11881 agaaaaagca agaaaaactc ttgaaactaa aaaagaaggt taagaaaaat tatgattcta
    11941 atattaataa gatagactca tatctaaaaa ttttagagaa aagtataatt gatgagtctg
    12001 atcacgttga gttggttgta tttaaattat atttgcaatt agaaaatgtt gtaaaagtaa
    12061 ctgaaactgt aaacgactta ggatttagaa taaagactaa cacttatgca agagaaagaa
    12121 aatatagtac aaatgatata acctctataa tcacagatcc ttttactgat gtagttgagg
    12181 acttaaaaat attagttcaa gagaggcaaa gaaaaaatta ttatggaaat aaggagtgtt
    12241 tttaatgaat ggaagcacat agtggacaaa aggttaatcc cataaaagat atagaggatg
    12301 tatttagact tttaaatttt ctagaagaat ggaatgaaag aaactattta ttagccctct
    12361 ttggaatgtg tactggactt agggtagggg atatattagc acttaaagtt gcagatgtaa
    12421 cagacttaaa actcgataaa aaaggaaata aaataagagt atctaaggat tggattagag
    12481 ttatcgaaga aaaaagagac tataatagag aggttttttt atctgatgta ataaaaagtg
    12541 ctatagaaaa ttatactcaa gataaaccag gagaagaatt tttatttaaa agtggcaaga
    12601 ggaagaaatt taatagacct atccaaacaa gacaagcagg aagaataata aaaggagcag
    12661 caaaaaaggt aggaataaaa gaaaatgttg caacacactc tttaagaaaa acatttgcaa
    12721 ggcatattta tgatgaagaa gagaataaga cttatgcatt agaacttata aggaagattc
    12781 taggacataa gacaatagaa atgactagaa gatatatagg aatagataag gatgaagaag
    12841 taaaagcaat aactaaattt actaataagt taaagaaaag aaaaagaaat taaaatatct
    12901 tgtattttat ctaaaattta atatgttacg tttttcaaca acaggacatt attagaatgc
    12961 aaagagcctt aacaatagag tttacaagga atagaataga tttattaaaa tgtctgaaaa
    13021 tatatattaa aggtcattta ttcaagggat ttatagggat gtagatattt aaatgtttat
    13081 ttagaagctg agtaaagagg tgattaattg gatgtgaatg aagttgaaat aatcgatagt
    13141 agctatgaaa aaggtaaaga gttgacagaa gatcagtgca atatggtaac tctattgatt
    13201 acaggtgcta cagtaacaga aactgccaga attattggag taaatagaaa aacaatctat
    13261 aattggatga ataaagagca tgttaggaaa gaaatggaca gacggaaaca ggagctaaca
    13321 aaccaaggaa atttaatgat attaaaggat ttagatagtt atatcaacaa tatcaaggaa
    13381 ttagctagtg acaagagcga taagagggtt atgttggcag ccaatcagta tcttataaat
    13441 cgtatatatg gaactcctac aagtacagtt atacaagcag aggataatag tgttggcatg
    13501 ggtatggatg caacagagat agaagcagct atagctaaga taagaataag aacaagcaag
    13561 aagtaattag atatgaaata aatcctgcaa gaatatgttt taattatgtt tgaaatcaat
    13621 taaagcaaaa catatattat atatatattt actttaataa ttaaatcata tattgtattt
    13681 aactttagtt atagctaatt taataaaatt tagtgagact taataattaa agtttcatac
    13741 agtgaagtga tgtggattat tataggatat gtgaacctat tggaaatgct aggttctttt
    13801 taactatgcc gaaaatacat atttaaggaa tagttgaaag ataattaata attctaatca
    13861 aaaaaatatt tcttgcaaga aaataattca aaatacttta atatatttat tttttttatt
    13921 aatagggggt gtccttctat tttgaaaaaa ttatttttta tggaaaatga gttctagaaa
    13981 ttttctagca aaatttaaaa accagagatg tatttcaaag agggtgattt tattagagaa
    14041 aaatttacat tgtttatttt attaattgca ttactcactg agcctaaaaa aatagaagaa
    14101 gtaagaggaa gtaaatacga gtggataatc tagaaagaaa tatacaatta atatttagtt
    14161 atctagcaaa gacttttata agaaatggag catctataaa agatgctgaa aaagaagcaa
    14221 atgagattat aaaagccaat tcaaacaatc tttggggagt taatggtctc gcttatcaac
    14281 ttggaaaaat aaacctagaa tttttctgta tgtattttat gcaagacaca tatttaccaa
    14341 aagaagataa tgctgctgct cctatagcta aagttcatca tgagctatgg gaagatatac
    14401 aagaatctat aattggagat ggttcacagc aattaggaag aatttatcca agagggacag
    14461 gtaaaagtgc atttggagat ttagcgacaa ctgtatggtc gcattgctat aaacataaga
    14521 catatacttt gatttgctct gacataggat ctacagcaga gaaatttgta aaagatataa
    14581 aaaatgcatt acttgaaaat gagtatatta aaaaagcatt tggtgtcctt ttaaatgata
    14641 atgatagaaa atacatttgt aatagtactc aactagagct aacaaataaa acttttattg
    14701 aagctatatc atcttcatca cccatgagag gaagaaaata caataataac cgtccagatc
    14761 ttatcatact tgatgattat cagtcagaag aaaatgttag aacagaagat gctagagaga
    14821 aaaaatttaa aagattttct gatgatgtaa aatatgctgc tcaaaaacca gtcataagaa
    14881 atggtaaaac tataaaaaga ggaacaactt ttatagcatt aggaacctta caacacaaag
    14941 aatgttttta tagtagacta aaaaatttac caacttggaa gtttaaatgc gaaaaaggag
    15001 ttttagttga taatgtagat gaattattta attctggttt atggcttaag ttcaaagaga
    15061 tcttatttga ttttaaaaat acaaatcatc ttgaagatgc taaagaattt tactgggaaa
    15121 atgaaaaaga aatgaaattc cctatattat ggccaagttt ttgggattgt ttagatatgg
    15181 ctctaagtta ttatgaaaat ccaacatctt ttaagcagga agtgcaaaat gatgtcaatt
    15241 ctataggaga aaaatggttt aaaactgtta gaacagaaac tagagaagaa attgaatcgc
    15301 atacttttaa aaagacaatg ctgttatgtg atccagcatc tgcgggtggg tcgaaacatg
    15361 actatagcgc ttttcttgtt ggaagtgaat cagaaaatgg tttactatat ggaagattgg
    15421 cagaactagc taaaataaat gctagaactg attttgataa atatatagac catatgattt
    15481 atttattaaa agtgtattca gatataacac atatttatat agaaaaaaat acatttaatg
    15541 gcgcagatgc caaccaatta gagtttaaaa taaaaaatga taatgttctt agttatagag
    15601 atatagaaat cattaatgag caacaaaaga aaaataaaga tgataaaatc tctactttaa
    15661 tacctgtttt aagcaaaggc caaatgattt ttgcagaaga agataaggaa tttatacagc
    15721 aaatacttga ttttacagga caaaaatact ctctacatga tgatgctccg gatatatcat
    15781 cggaatttat taatcgaatt aacaacataa aagtttctga gacagtaaat atctttgata
    15841 gaaaattatt aggtttttag ttgggaggta atttattggc taatagatca aagtttgaag
    15901 aagttcaaaa aactttaact aaaaaaggaa agaagttcaa tgttggaata ggaaaagatg
    15961 aaaaaggata ttttgcatat actcatagag caaggagtaa aagttatatt tcaaagcaag
    16021 atattccaat aaaagtttta aaatttatag agtctacagg ataggatgtg ttgttaatgg
    16081 aattagattt agacttaata aaagacatat atgacgattg gaactctaat aaatccgaat
    16141 atgatactat gtataagtat tataaaggtg aaacggatgc tattagcaac tataaaatgg
    16201 ttaccaagag atctaataat aagataaaca ctaatttctt aaaaaagttt attaatgaag
    16261 aagtcgctta ttctcttgca aataagatta cctatacaag tagatctggt gatgagaaga
    16321 ttataaatga tttagaatac tatacttgcc attggagtaa aaaacatgac tcagatttat
    16381 tgaggtatgc actattattt gggctttgct atgaacttta ctatgtaaaa gatgatgaaa
    16441 tacaagtaaa gataatcaag ccaacagatg gctgtcatta tgaaaatgaa aatggagaaa
    16501 tagtatgctt tattagagag tttaccaaag gtttcgaaga tgacacgtat gtagatgtat
    16561 atgacaaaga atatatatat cattttgatt caaatttcaa agaagttgaa agtcctacag
    16621 taaacaatat atttgatgga aatgttccaa tatcaatttg caaaagaagt gaagaactag
    16681 gaaaaaatac aatatttaat gatataaaag ggttacaaga tgcttatgaa actaatttaa
    16741 gtgatataag taatgaaatt agtgatttta gaaatgctta tttaaccttt tctggatgta
    16801 atattaaaga agatgatttg ccaaggatga aagaacttgg aatacttcaa gttaatggag
    16861 atggaaaaat agagtggctt attaaagata tgaatgatac atttgttcaa aatacactat
    16921 cttcaataaa agaaaatatg tatgaaatta catctcatat aaatcacaat gaaaaaatgc
    16981 aaagtaatac atcaagtcta gcagttatag caagattaat cagtacagaa tggatttgta
    17041 gccaaaataa tgatagtatt gcagatactt tatttaatag gtacaaatta ttgtgcattt
    17101 ggcttaataa aaaatatggt tttgactatg attataaaga tattaaggct aaatttaccc
    17161 ctaagatacc acgtgatgat ttagttgtag caaatatatt aagccaactt ggagataaat
    17221 tatctactga aactggctta tctcaactaa gtttcattga taatcctcat gctgaaatag
    17281 aaaaagctaa aaaagaacag gaaatagttt cagagggaga gattttatta gatgaatcaa
    17341 aagattataa ctaaattgac tgaagatata tataaatctg ctgaaaatag gacaaaacca
    17401 ttttataagt atcaaagaga aaatagggat aaacttttat tagaaatagc aaagatattg
    17461 ctaacacacg atattattaa tgaatactta aatattaata ataaagataa gaaaaatcta
    17521 aggtcaaaac ttaataaaat tataaatgag tattctaata gttatgatga agaagtcaag
    17581 gatatagaaa ctattttatc tgattcatct aagaataaat attcaaattt gataatttta
    17641 ttaggagtaa tactgaagat aaaagatgtt agtaatataa acaataaagt tataaataag
    17701 atagtgaata ataaggttga tggaaaacat tggagcgata gactctggaa gaataaaaaa
    17761 ggtatagaag agtccttaaa aaaagaaata aagagttttc taaatggaaa aacttctgta
    17821 aataagatag aaaaagcaat aagagataaa tattcaacag gtgcaggaca aaccagcaga
    17881 ctaatagaaa atgaagttgc cagatgtcaa agtgaagtaa atgagtattt tttcaaggtt
    17941 caagggatta taaaagtaat gtattgtgca gatttagatt ctcgcacatg tagtgattgt
    18001 tctatgcata atggagtgat attttatgtc aatgaagata ggccctcttt gccaagacac
    18061 tgtcactgta gatgtcaata tatacctgtt taacatgtat taattaattt aataggtgtt
    18121 tttattatgt ctaaaaaata tgaggtgaaa tatgaaagtt gaaatattag gaacagaata
    18181 tctagtgata gagaaacttg aaagcgaaga tgtgctatta aaggaaagag ctggatattg
    18241 tgatcactct gttaaggaaa ttgttataga gaaaataaat tctgaagaag gttcattgaa
    18301 agatttaagt gtatataaaa atgaagttgc taggcatgaa ataattcatg catttcttag
    18361 tgaaagtgga ttgaagagtt gtagttcttg ggctacaaac gaagaaatga ttgacttctt
    18421 tgcaattcaa tttcctaaaa tagtaaaggt tatggataaa atagaatgtt tgaattaaat
    18481 tttcaaaaag gagaacttta tacatgttaa aaaaagaagt gttggaatta ctaaaagaaa
    18541 tagaagatga tgcagaaata gattcattgc tgcaatcaac agatttattc aaatctgcat
    18601 ctgataaaaa gcttacaata gaagagttta gagaattaat taataatgat gcaaacttta
    18661 aagctattat agataatgaa aaaaataaat atcacagcga agcacttgaa aactttaaga
    18721 aaaaagatat gcagacactt ataagtgctg aagttctaaa aagaactgga gcaaatgaaa
    18781 cagaagagca aaaagcaata agggaattaa gagaaagcct agacaaacta aaaaaagaaa
    18841 agcagtacgc agaaaaagtt tctaaatata aagatatttt agttgaaaag aaaataccta
    18901 caaacttaat tgaatatcta ttatctgatg atgatgataa aacaaatgca aatatagaga
    18961 tatttgaaaa ttcaatgaag caatatgtgc agtcaaaggt agatgaaaga attaaagatg
    19021 gttcatacgt tccacctggc aaagatagtg caggttcgct atcagaaatt agaaaacaaa
    19081 taaagcaagg gctaaatagt ctttagtata aaaaaagaaa ggatgatttt ataaatggct
    19141 aatgtattac aatatgtaac actttttcaa caagaactag atatgcaggt aacagttggc
    19201 tcaactactg gatggatgga gaaaaatgct ggtcaagtta tatataatgg aggaagagag
    19261 gtaaaagtac caaaagtaac aatggatggc ttaggtgatt atgacagatc taaaggatat
    19321 acgcaaggcg cagttacact tgagtacgaa acaatgaaaa tggagatgga tagaggtagg
    19381 acttttatgt tagattcaat ggatgtaaat gaaagtaact tcgttataaa tgcatcttct
    19441 gtaatgggat tatttcaaaa gcaacatgtt ataccagaaa tagatgcata tagatactca
    19501 aagatatttt ctgaaacagc taaagtagat gagcgtgtaa aatattcata cgaagctaca
    19561 gtaactacaa tattaagtga attgaaaaat caacttgcat caatacaaga tttatctgga
    19621 gatatacctc ttattattag tatgtctagc ataattgcaa gcatattaga acaatcatct
    19681 caacttgatg atattgaatt tggcgatgga gatataaaaa gaattgtaaa gtctatagac
    19741 aatgtaccta taataaaagt tccatcaaaa agaatgaaaa cagaatacgt atttagagat
    19801 ggtaaaacat ctggacaaac agagggtggg tttactgaag gagcaaaggc tttagatata
    19861 aactggttaa taactccagc atctgctcca attgcagtat ctaagactga taagccaaga
    19921 atatttactc cagatcaaaa ccaaacagca gatgcttatc aaatagacta tagaaaatat
    19981 cacgatttgt ggatattgga tgaatattta aaactttgta gagcatctgt gagacaagct
    20041 aaagctgcac aagaataagg agttgtttta aatgtttgca ttgataaaaa ataacataat
    20101 taaagttgtt gaagatgaaa attcgagaga taaattgatt tcaaaaggat ttaaaatata
    20161 ttcagaagaa gtgactaaag agaatattga gaaaaaagaa gaaaaagtta aatcaacctc
    20221 taatgattta tttaatctat ctaaaccaga gcttatggaa attgctaaag gcaagaatat
    20281 aaagggatat tcaactaaaa ctaaagagga attaattcaa ctactagatg agtacaatgc
    20341 ttgataatgt taaattagtt ttaggaatag aaaatgatag atatgacaaa ttaataatcc
    20401 tttatataaa taaaataagt gagatggttt tagaatattg ttctaggcag gaactaagta
    20461 cagcactaga agggtttgta gaagaaaaag taatatcaat attatcttca agatttgaag
    20521 aacttaagaa aaatagtgga cttgataatc tagaaaaaag cttaaaagct ataactagag
    20581 gtgacactaa gatagaatat aatgctttaa cattacaaac tgctgaaaat agcatcagtt
    20641 catcatcttt tttaactgat actgataaaa aatttctaag cccattttgt attgttagta
    20701 tgtactaagg ggtgattaaa tgactgaagc agatatatta gaaatgacat accttgataa
    20761 aatgactata attagaaaat taaaaggctg gaatgaagaa acatgttcta atgattttag
    20821 taatgttgtt atagctgaaa atatcccatg tgcaatttct agaaaagaag aagccattgt
    20881 aaatggtgat attggatcta tagttgtaaa tagaaagtta ttttgtagac cagaagtaga
    20941 tataaaaata ggtgatacta taaatacaac ctataatcat ggagaagaag ctgtatttaa
    21001 ggtttctaag gcaaatatat atccaagcca tatggaaata cctattacag aattggagag
    21061 gatataatgg gaatagataa tttaggagtc tttgaagcat tagccttaga tttaggtcta
    21121 aatgcatcaa actttaatag aaaaatcaag aagtttacaa aagaaactgg gcaagaacta
    21181 gcagaaaata taagagatga aactcctgta aaaactgaaa agttaaaaaa gggatgggaa
    21241 gaaaagcaac cagaatatga tacttatatt gttaaaaatg aagttgatta tgctacttta
    21301 gtaaataatg gccattcaac taaaaatggt ggcattgtcc ctggttcttt tatggttgaa
    21361 aagggaattg aaagaacaaa agaaaatatg acaagtgcaa aagcaaattt atttgattgg
    21421 taggtgatta gttgcttaca aacagagaca ttataatagc agttaataaa agaatcgaaa
    21481 atgagtttag atatatagat atttttatta atgaggataa tatacaaggc tttgaggagg
    21541 cttgtttttt tgtgcaactt ttacctgttt ctaataatat tgcaaataaa gttttagata
    21601 ctaaaaagat attcatagat attgaatatt accaaaaggc tagaaaaaat aaattaaatc
    21661 tatacgacat acaaagtaga ttagaaaaaa tatttaacag aaacattaga gttaaagata
    21721 gaagtttaat cattgagaat atatctccat caatcgcaaa agatcctgta ggatataaac
    21781 taacttttct aatgactgta tcttattttg aagaagtcta ttttgaaaat gaaacatttg
    21841 aaattatgga aagtgtagat ttagagatag gaagtgatta aatgggttta ccagaaatta
    21901 atatttcttt tatccaagca ggaaaaactt ttgttaaacg ctctggtggt attgtagcgc
    21961 ttattttaaa agatacaaaa aatatagggt tagttgagat aaaagaaata gaggatattc
    22021 cagaaaatta ttctgctgct aatttagatt atataaaaat ggctatgaag ggaaatgtat
    22081 atcctccaaa taaagtatta gtttatacat tagatacaga tgaatcaata gatgatgcac
    22141 tagattttct tgaaacttgt gagtttaatt atctttgtat gccagatgag acagaacaag
    22201 atttgcctaa aatcaaaacg tggattaaga aaatgagaga agataataaa ataaaggtta
    22261 aagctattac tgcaagtaat tcagcggatt atgaaggtat aataaacttt acagcaactg
    22321 atattgaagt tgaaggtaaa aaatacactt caaatgaatt tctgccaaga atagcaggtt
    22381 ttattgcagg aactccatca actcaatctg taacatatgc agaaatacca gaagttacta
    22441 atatacctaa gctaacaaga gctgaagcaa atacaaggat aaataatgga gagctaattt
    22501 taataaaaga aagtggagct atagtaatcg ctagaggagt tacatccttt acaacaatta
    22561 ctgattcaaa aggtgatata ttcaaaaaga ttaagttggt tgatacacta gatcaaattc
    22621 ataacgatat taaaaaaata attgtaaaaa attatatagg aaaaacccaa aacacttatg
    22681 ataataagtg tttacttatt gttgcaatac aattatatct tcaagaatta gaaaaagatg
    22741 gtttaattga tgaaggttca actgtagaaa ttaatttaga tgctcaaaga gcatggctaa
    22801 aaaaacaaaa tatagatata tctaatatgg aagaacaaca gataaaagaa tacaatactg
    22861 acactcttgt atttttaaaa gcaagaatta aattaataga tgcaatggaa gatgtatatt
    22921 tagacatctc tatgtagttt ggaggttaaa atttgaaagc taaaatagat gcaaaaagaa
    22981 taatatctgg tacttttgga gagtgttggc tagataataa gcaacttaca tcaacaaaag
    23041 ggatggaggc caaggttgat atagataaag aagaaataaa attatgtggt caaataatga
    23101 ccacatataa aatgattggt gcaactggaa aaggatctat aactctttat aagataagta
    23161 ccttatttgg cgagtatgtc gaaaaaatgt taaatgaagg taaagaattt aaagtaacta
    23221 taataagtaa attgcaagac ccagattcat atggttctga gagaattgca ctttatggat
    23281 gtatgtttga cgacttaact ttggctgatt gggaagctgc gaaagttgga gaaatagaag
    23341 ttccattcac atttgaaggc tttgaatact tagataaaat aaaagaaaaa taggaggatt
    23401 ttattatatg aacacagtag aaaaattatt aaatatggat gcaggtaaat taaaaatgcc
    23461 atctagcgca tttgaattat attgtaaaaa actagatgat actttagaaa tagagtgcaa
    23521 ggctattgat ccagaacgtt ttgacgaaat aagaatgaac tctatggacg ttaatagtgg
    23581 agatgtagaa aacattaaca tatatgaact taaagtaaat acaatattag aatcatgttc
    23641 tatatttagc aatatggaat tagttaaaaa atttagtgca attacaccta aagaattaat
    23701 taaaaagcta ttacttagtg gtgaaataga taaattatac gaggaggtta ataaagtaaa
    23761 tggaatagat gcagagggag ataaaaaaag acaaaaagaa agagctaaag atataaaaaa
    23821 ctaattaaaa cggatggaga agttaacttg atgtacttat tatttaagta taaaggtata
    23881 atgccatccg tttcttatca tatactaaga ggcgaaaaag aaattgttag tgtttttatg
    23941 gatcaagaat tagatgaaag aaaagaagaa ataaaatctg gtgttgggag gttgttttag
    24001 tatatgagtt ctaatacaaa ggtcctacaa gcaataataa aaatgagaga tgaagcaagt
    24061 tcaacattaa aaagagtagc agcaaatact agagctctac atgattcatc ttcccaagca
    24121 tcaagagcat taaataatgt tcaaagtaat ttaaaaagtg ttggaactag tgctttaaaa
    24181 gcaggaggtc ttatcactgc tggagttggt ggtgcatttg cagctgcaac taaagttgga
    24241 acagaatttg aaagcgctat gtcgcaagtt aaggctacat ttggaaattc cttaaaacca
    24301 ggagattttg aagctctgtc taaggctgct agagatgctg gaaaagctac aactaaaact
    24361 gctgctgatt ctgctaatgc acttcagtat atggggcttg ctggatggga tgttcaaaag
    24421 tcaatgcatg ccttaatgcc agtgcttaat ttatcagaag cagcctctat ggatcttggt
    24481 ctagcatcag atttagtaac tgatagcatg agttccatgt gcttagaagt tgataaggtc
    24541 agtaaaagtg gaaaagtact agctggtgca ggtttaagtg agtatttaga taaagttgca
    24601 aatgcatcta ctaaatcaaa tacaagtata caacagttga tggaagcgtt taatgtcgca
    24661 ggtggtacat ttagcaattt aaaagttcca ctttcagaag caactgctat catgggaata
    24721 cttgcaaata gaggagttaa gggtacagat gcagggcatg ctttaaactc tatcatggta
    24781 aatcttacaa caggtgcagg tcaagcaggt aaggcaatga aaaagttagg tgtaagtgca
    24841 tttgataatc aaggtaaatt taaaggtatg tcaaatgtgc ttaaagaggt tgcaaataaa
    24901 actaaaggta tgactgatga acagaaaaat tattacttat ctgctattgg tggaaagact
    24961 caattaaaaa cgttacaaaa gttacttgat ggtgtgggaa atgaatatga tagcttaaag
    25021 aaaaaaatag ataattcaaa aggtgcttta aaaacaatgt ctgatgtaat gaaggataat
    25081 actaagggtg gacttgcaac cttaaaatca gcccttgaag aaactggaat tactatttac
    25141 tatcaactaa agccagcaat agcatctgta gttgataatt taactaaaat gactgtttgg
    25201 tttaataatc taagtgaacc aataaaaaga gtcataacta atgtaatgct ttcagttgga
    25261 gcatttggag tacttttatt aactataggg gctgtgtctt taattgtttc aaaagctata
    25321 ggtatatttg gcttttttgt tccaataata ttaagagcaa aagttgcttt cagagctttt
    25381 gtaattggta tgaaaccatt aagtgcagta tttttagctg taatgggtcc tgtgggatgg
    25441 actataacag ctgtaattgc attatcagta gcatttacca ttgcttatgc taaatctgaa
    25501 gtatttagaa ataaggttaa ttcattagta aaatcattta cagaatttgc aaagcctata
    25561 gtaaattttt gtatgcctgc acttaaacag ctaggagaga aatttaaaga gttgttatca
    25621 gcatttggtc cactatttgc atctatttta aacctaggaa gtgtaataat gaaggtacta
    25681 ggacctgttt taatcggcgt aataggtttt catatagcaa aattagtttt gattttatct
    25741 actctggcaa ctgcttttaa agctgtagtt gatactataa ctaatgtatt aaatggtcta
    25801 gcatctattt taaatggagt atttgattta attataggta ttgtcacagg cgatagtaaa
    25861 aagatgatgg atggagttaa acaaatattt gatggtggaa ctaaaattat aaaatcaatt
    25921 tggaaaggtt tcatagattt tgtaactgct cctatacaag ctatagctga tatagctgac
    25981 agcttattta aagataaagc taaatggctt acagataagt ggagagattt aagaacattc
    26041 ttagaaaatc caattgaagc aactgccaaa atatctacta aaacaaatgt aaatatccca
    26101 ggaagtaaag cagcagatgc agctggtaaa tctacaccaa atacaggtgg attaaaacaa
    26161 acagtagcaa atctaaaagg ttcatttagt gattttaaaa cttttattaa gcctatgaca
    26221 gactctataa gtcaaggagt tgaatctgga aaaactaaat ttacagaact gcaaaataaa
    26281 atattaaatg atgttaaacc tgcactaaag gtcctagagg attctggagt aaattttaag
    26341 gataagttta aggagtcaat aacttcaata aaagataaat ttaatgaatt aaagaacata
    26401 ttagctggaa atattaaaac aaatatagat aatttagtaa aagcatttga acctttaaaa
    26461 cctcatttag ataatttgaa agattgcttt gataaggtta aaaaagcgat tactgatttt
    26521 tttactccag ccaaacaggt tggagataca gtaaacaaag taagccaacc aatggatagt
    26581 gttaaaaagt ctactgatgg aattaaactt tctttccaaa gtttaactca agcacttgct
    26641 cctgttaaag cttggtttca aggatttatg cctttcctat cacaaatagg acaacaactt
    26701 ttaccatcta taggaattgc aatagctggt gttgttggcg gaataatttt agcatttaca
    26761 tgggttttaa atacattgac cgcaataata acaacagtat ctggaatcat aaatggaatt
    26821 atgtctttta tatctggaat tattaatgtt attatggggc ttataacagg aaatgctgat
    26881 caaactatgg atggacttaa acaaatattt gatagtggag taggtgtaat aaaatcaatt
    26941 tggaatggcc ttgttgatct agttagtggc gtactagaac ctgtaataga tcttgctgat
    27001 tctcttttca aagataaagt taaaggggta aaagaagctt ggaataaact taaagatttc
    27061 tttaaacatc caattaaggg aacaatagaa atatttaaaa aaggtcctag tttacaaatt
    27121 ggaaaaggtg cagatgctgc tggcaaaagt ccaagaaaag catttggtat aaatagagtt
    27181 cctagaaatg attatttaat aagagcacac gaaggcgaga aacttttaac caaacaagaa
    27241 gctaatcaat acaaaaggaa tagttcaaat ggaagtttat ttcttgataa attggctgat
    27301 acaataattg taagagaaga ggctgacata gataaaattg taagaaaaat aaacaaaaaa
    27361 atatctcttg caaaaattgg aggtgttttg taaattatcg tgctataatt gtagtatata
    27421 atttacaggg ggttatgtta tgaataaaaa aatactagta ttaacaatat caattatact
    27481 atctttaagt ttagttggat gcagcacaaa taattcaaaa aatagagata gtaaatctaa
    27541 gcaaaacaca gaggaagtta aaaatcataa aataggcgaa gttgttgatt tagatggagc
    27601 aaaagtatct gttaaaaata ttaggaaatt agaaacaaat agtaatgatt atgctgttga
    27661 aattgaaata actaatacag gtgataatga tttacaaagt gattatctag aaaactatat
    27721 attaaaatca agtgacaata ctactggaga agcaataaat aaccaagaat taaatggaga
    27781 gttaatgttt gatacaatac gcccaggaga tactttgaaa ggtgaaatag cattcgaact
    27841 tacaaaagat gcaacaccaa aaacacttga aattgcttat gaaaatgtag gcaaatatac
    27901 agtatttgat ttatagtaat tatcttaaaa gacgcttatt tacttaagtg tcttttattt
    27961 taggaggagt ttaatggaaa tatggttaag acaagcaaat aatacattta ggtttcctgt
    28021 tattccaccc tcttttgaga taaatggaag tgcgacaata aatacatcta atattttaag
    28081 tgttggagat atcgcagtat ttggaggatt agggcttaaa actatagaac tatcctcttt
    28141 tttccctaat caagaatata gtttctgtaa ttataatgga tttccaaaac catatgattg
    28201 tgtaaattta atagaaagtt ggatgaaaga aggatatata ttaagattta tcattacgga
    28261 aactaatata aactttgaat gtataattgc agattttaat tatagagaac aagattattc
    28321 aagagatatt tattttacct taagtctaaa agaatacaga aggatacaaa tatccaaagt
    28381 aaacattaat aatgatgaaa aattatcttc tgaaaagaat gttcctctaa caaaaggatt
    28441 tgatactaag caaaaaacgc ataaggttgt tgaaggagat actcttttta agatagctaa
    28501 aaaatattat agcaatggag atttatggga gaagatttat aaagcaaatg aagatgagat
    28561 taaagatcca tctgtaataa aaaatggttg ggtattaata attccttaaa aatggtggtg
    28621 atttataatt aataaaatca aaatacaagt tcatataaaa aacggaaata tatataatgt
    28681 aactgattta gtggataaat gcacttggtc tggtgattat agatcaccat caagaacttt
    28741 ggaattttct ataattcaat ctgcaagtga tattaatttt aggcagatag atattccagt
    28801 agcaagtaca gtttgtttct atgtagatga taaagaactt tttagaggaa tgataataga
    28861 tagatctaaa gactctagca ataataatat agattttaca gctaaagata tgggtttttt
    28921 acttttacaa agcgaagtgt catacaattt taaagataaa ttagtcgaag atattgcaaa
    28981 acaggttttt gtagataata aattcccact aggtaattta cctaaaacta acgttaaata
    29041 tacaaaaatg tttattggtg taactggcta cgatactata atgagcgttt atacagaagc
    29101 aagcaagaca actaaaaaaa agtatatgat agagtctaat cttgataaat ttaatgttat
    29161 tgagaagggt gtagttactt taaatataac atttgaagaa ggctttaatc ttatcaatac
    29221 aaatttttct gaaagcattg agaatgtaaa aaataaagta ctagttgttg atcagtatgg
    29281 taataaaatt agtgagaaaa taaatgactc tatatttaaa gacgttgggg taattatgca
    29341 aaaggtaata caacaacaag aaaacagtac tatagacata gatggagagt ttaaaggaat
    29401 cgaaaaaact tgctctttaa aaggatatgg tgatataact tgtgtaactg gtagaggtgt
    29461 aaaagtaaaa gactcatata caaaattaat aggtcttttt tatatagata cagataagca
    29521 tacatggcaa aatggagatt atcaaattga actagagctt aattttgaga atataatgga
    29581 tgaaaaatct gctggccaag atgagcaaaa agaagatggt tcagagtata ctggaggaac
    29641 agaataccct gctgaattta cggcttattg tcctagaaaa gaagagggtg gaaatacaga
    29701 ttgtagaggt aaaaaactag atccttctaa aaagacatgt gccgctccta tggttggagc
    29761 atatgaaaaa tcttactata caaaagattt cttaaacaaa catcctctta tgagatatgg
    29821 agatgaaata caattagtta caggtgtttc aagccgagat ggaacgtata aagtaaatga
    29881 taatgggcct gcaataatta tagaaaagga tggaacatat catatagata ttttatttgg
    29941 aaatgttgaa gaagctaata actttggaag aagaaaagga aagattataa ttggtggtta
    30001 ttctggaaac atgacagaaa aagcaaaaac agtaatttca gaagcaagaa aacatcttgg
    30061 aaagccatac aaatggggtg ggaatggacc aagtagcttt gattgttctg gactaatggt
    30121 atattgcttt aagaaggtta atgttaattt accaagaacc tcaagtcagc aatctaaggt
    30181 aggtaagaaa gtagagaaaa ataatttaca ggcaggagat ttggtatttt tccataatcc
    30241 agtcagccat gttggactat atgtaggtaa tggagaattt ttacatgcgc cacaaacagg
    30301 agatgtagtt aaaataagta aattaagtaa tagaaaagat tttaatacag ctagaagagt
    30361 tttgtaaaag aggtgatatc gtggcagatc caatcaatga atttatagga ataattaggc
    30421 aagaaggtaa atttcataat gaaccttctt tttttattgg aaaaattaaa agtaaattac
    30481 cagatttaaa aatagagata aataacatca tattagaaaa agaagatatt ttgatagata
    30541 gttggatgct tgatagacag atagagttat ttgatacaga aacaagtcaa gaacataaac
    30601 atgaaataaa aaatcccttt atagatactt ttgaatctgg aaacacagta ataatgttta
    30661 aaataggtga aaaatttgct gttgtaagta aattggtgag tctagatgaa taatactata
    30721 ttccctttca tgggtgttcc agaagattat atttctccag acaatgaaga attacctatt
    30781 tttaaagaat ttgcttggga ttttaataaa gatgaaaaga taattgaaaa tggagacttt
    30841 aaaatagttg aaagaaatga agcaattaaa gtatggattt acaaaacaat aaaaacagat
    30901 aagtatgttc atttaatata tgattggagt tatggaacag atataaaaaa tctaataggg
    30961 caaaagtata ctaaaggtct tactgaaagt gaggcaaaaa ggtatataca agaagctcta
    31021 ttaactaatc catatatatt agaagtcaat ataacaaatg cagaatttga agatgataat
    31081 ttatctataa gtttaaatgt aaaaacaatt tatggagagg aggagattac ttttgtataa
    31141 tggccaatca ttcagtacct taaataatag aactttaaat aacataaatc tccctcttta
    31201 taaaggccaa ggatcttctc tttataatat agtttctcca ataaattcag aacttgcaca
    31261 attatatata gaactttctt atatacataa gagagttttt attcaagata attttgacga
    31321 ttttcttgat agaagagtca atgaatttgg cgtatataga aaattaggta cagaggcaat
    31381 aggtgaggta acatttgagg gcaaaattgg aactcaaata ccaaatggaa caataatatc
    31441 tcatagtgat ttactatttg taattattaa agatattaca atagatgaaa atagtaaatt
    31501 aaatgtaagc cctatacagg ctttagagat tggcattaag tataatttat cagctaacac
    31561 agaatttaaa cttatagaag aaataactgg agtaactaaa atttataatg aacttgacct
    31621 aaagggtgga acagaaatag aaactgatga agaattaaaa gaaaggttct ataaaataca
    31681 aaaaaatcaa gcgacaagtg gaaataaggc gcattaccaa tcttgggctt tagaggttga
    31741 aggtgtttat aatgcaaaag taattcctcg ttgggatggt ccaggtacaa ttaaaattct
    31801 aatttatggt caaaacaatc aatcagttga caatgaagta ttgcaaagat gtactgaaca
    31861 tatagaagaa gaaaaaccta ttggacctac tgttacaatt gttacaccaa gtacttttga
    31921 tattaatatt agtgcaactt taacacttga aaatggatat gatattgaat ctataaaagt
    31981 tgtgttttta gatattataa attcttattt gatagaaaat tcaagagaga ttgtttatat
    32041 aaaagtaatg agtttgttag caagcataga aggtattcat gatataaaaa atctccttgt
    32101 aaatggtgat gttaaaaaca taatagttga tgaagaaaaa gttcctgctg tttcaagtgc
    32161 tatatttgat atcgaggtga gttaaattga agttaattga ttacctgcca agttttgcaa
    32221 acaatgaaat tgacatacaa atccaagaag cacttgaaaa tgaactttta acgctaattg
    32281 atgaaaaaga tgatttatta gaacagtttt ttatagatac agcaacttgg ggacttgatg
    32341 attgggagaa ccttttaagc ataaaagtaa attataagtt agacttcgat actagaagaa
    32401 gcaatataaa agcaaagatg agaggtaagg gtacaacaac aatagaggtt ataaaagcta
    32461 tctcggaagc ttacacaaag accaatgttg atgtagaagt atttagtaat ctatttagtt
    32521 ttacacttag ttttataaca aataattgta gttataacac tattttagaa ttagataaga
    32581 aaatagaaga aataaaacct gcacaccttg aacacaaatt cgagaggata ttatttaata
    32641 aaaacgagct ttatacaggt gcagcaatta gtacaggaga aacagttaca atatatcctt
    32701 atgtacctag aaatttagaa agttttggag aaatagctat ttgtagtgga aatgatagag
    32761 cattagaaaa agtaacattg tatcctaaaa aatagaaatg aggtgataaa attgacagaa
    32821 caacaatatt ttactctagt aactgacatt ggtaaggcag caatagcaaa tgcaagtatt
    32881 acaggtgaaa aagtagattt tgcaaagata aaagttggag atggaggagg gagttcttat
    32941 actccaaatg agagtcagac agcactaaaa aatgtggttt gggaaagtac acttgaacat
    33001 gcacaaggag ataaagataa tcctaactgg gtagtaatac aaaaattcat acctggtgat
    33061 gttggaggat ttgaaataag agaagttggt ctatttgatt ctaaagacca attattagcg
    33121 atttctagtt acccaacaac atataaacct actgcggatt cagggactgt aaaagaacta
    33181 ttaataaaag taatattagt tgtatctaat gtagctaata ttaatttgaa agtagaccca
    33241 accgtaattt tagcaacttt aaaagatata caagaattag acactaaaat agatacaact
    33301 aaaacagaat taacaagcaa catagaaact gctaaaacag agttgaacac taaaatagga
    33361 gatacaacac aacttactac aacagataaa acaaatatcg ttagtgcatt aaatgaggta
    33421 aaaactagtg tagatagtat agaaacaaca gcagataaaa caagtataaa agatacagat
    33481 aatttatttg agagcgacaa cgtagaggga gcattaaaag aagtgatgca agaagtaaaa
    33541 ggtaatagaa gcagtattat atcaacaata aatagtaatt taataccaat gtagaaagtg
    33601 aggtgataaa gtgaagatat ggaaatacaa tagaactgtg aagaaaagaa gaggtgtcta
    33661 tacagaatta gatatacttg cacaaaggta tgaatttgaa gcagatggga aactttatac
    33721 agcttatttt catttatata ctaataagaa tatagatata ttcagagata aagatttata
    33781 taaagaatct tatatgatga gctatagagt tggaaatgta acaactgata ttgatgatgt
    33841 agtacagcaa acaactacag taagatttat acctgaccct gttattgata ttgtgattga
    33901 taacattagc agaggatgtg aaataaacta cagaattata gataatgaac caagtgtaaa
    33961 attcattgtt acagaaaagt taaatggcac aataatagaa acaaagacaa attctagtga
    34021 taataattat aaacttaaat taactgatga acaaatagta ccattagcat tcaattccca
    34081 aaattcaata gtaatagaaa taagtacaga agatggtgga ttagtaacaa gtaaaacagt
    34141 aacatttaca agaacaaata acaagccaac aattttagtt aattcatata gttctaattc
    34201 tgcaaaattt acagcatctg atttagataa taatttaagt aaaattgagt ggtatttaga
    34261 tgatgtatta aaagaaacaa taacaacaga tttaacagca gaaaaaacca tcaactatga
    34321 atttacagac aatgcaatac acacattaaa aatagttgct acagatgcag aaaatgcaac
    34381 agttgagaag gttttaagta taagtaaaga gataatgcca cttcaatctg atgctagttt
    34441 aagtgatata tcaactaagc tgatagagat tggagaaggg tttagaaatg gtaaaacaag
    34501 tattataaac actttagcac ttaaaaatat agaagcaagt ttaaataata cacttgtgga
    34561 gttatcagag aaaataaaaa caagttttga tagttcagac gctagtgtgc aggatttgca
    34621 aaatagaata acagaattga ataatcagct tagtcgaaga aaaaaatggg ctacaggtag
    34681 atacacattt actagagaag aagatactat caatttcaga atggttgatg gagatagtct
    34741 ttccgcagta aaaaaactaa ttatacccat taatttagat tttgttccaa gtgttattat
    34801 aatagacaaa atgtttttag gtagttacgt acctgcacaa gatgtgcaat ttaattgtgt
    34861 ttcaaactta gccgatttta cacttggacg tcgtttctat aataaacaat ggatttgcac
    34921 ttcttctata aaactagatt cagttacaca aaatagtttt actctttctt tacaaaaagt
    34981 tgttccattt gataaagtta gtagtacgat tttttctatt attaatactg gttttgcttg
    35041 gagagcatat gaataaaaat ataaattaga ggtgattaaa tgaatagaaa taatagaata
    35101 atttacgacc aaacaggcaa gatatggctt caaactggtg aagcaatagg agatatacaa
    35161 gagttgtcag aaataactga attaaatttc atagatgttg aatttggtag tatagactat
    35221 agtaaacaat atatagagtc tataaatcca gttacaaaag aattaaatat aaaaaatata
    35281 gaagttgttt tgactgatga acaaaagaga ttagaagtat tagaaaaaga actaaatatg
    35341 ttaaaagaag aaaataaaaa tagagattct gagatagtaa atacagcatt tgaagtagga
    35401 aatatcaaat taaacaataa tttataggag gaaattatat gtataactta ttaaagttaa
    35461 tgatagaaca aaaaaactat agcactaagg aggatttaca acataagatg gatgtattct
    35521 atgcagtaaa taggattaca gaagaacaat atttagagtt aacaggttta ttaaataaag
    35581 aagaaacacc agtagaacca acaatatagg ttctttttta ttgaaagaag gtgactaaat
    35641 gacttttaaa gaattagtta gtaaagttag aaatcttgta ttagaagcaa agaatgtaac
    35701 tatagaagat acagagaata aattcactag tgaaaatgtc gagggagcat tgaaagagtg
    35761 tatagataga gcagatttgg cttttcaaag tgccgatagt ggaaaagagt taattgcaac
    35821 tgctatcggc tctcctgcta catccgaaca aacatttcaa gagtatgcta attatattgc
    35881 aggatttaaa aatactataa gtcagttaaa ttctaaatat aaatatacta gtggaacttt
    35941 tacctattat actggtgcat atcctaatgg gctaaatata ggttttagac cagatattat
    36001 ttcctatact gccattaatg ctgtagagga aaaatattat tgtgtataca cacctttatt
    36061 ctatcttttt tataattata caactggaga aaaatatctt gatgttaatc ataatcgtta
    36121 tggaataact tttactgaca atggatggat acctagaaat ccagttaaca atggtgtgta
    36181 tagttatata gcagctaagt gccgttagtt gtcaaagact tagataaatt tctaagtctt
    36241 attttaatac aaaattagga ggaagtatga acgtaacaat agttttttta gcaacaaata
    36301 tatttataaa attagtaata ttagcaatag catttgatac attgctaggt tgtctaagag
    36361 caataaaaac acatcagttt aatagctctt ttggaataaa tggaggaata agaaaagttg
    36421 caatgatagc atgtatattt tttctagcag tagttgacat acttacaaag tttaactttt
    36481 tatttatgtt gccacaagat tgggttgatt tcttacgact aaatcatcta ggaatatctg
    36541 aatttttctc tattttattc attctatatg aaagtgtaag tatattaaaa aatatgtatt
    36601 tatgtggatt accagttcct aaacgattaa aagagaaaat aggtagttta ttagatacta
    36661 tgacagatga attaaatgtt aaaggaggaa aataaataat gaaaatatgt attacagtag
    36721 gacacagtat tttaaaaagt ggagcatgta cttctgctga tggagtagtt aacgagtatc
    36781 aatacaataa atctcttgca ccagtattag cagatatatt tagaaaagaa gggcataagg
    36841 tagatgtaat aatatgccca gaaaagcagt ttaaaactaa gagtgaggaa aagtcttata
    36901 aaatacctag agttaatgct ggaggatatg acttactcat agaactacat ttaaatgcaa
    36961 gtgataggca aggaaaaggc tcagaggttc tatattacag taataaaggt ctagagtatg
    37021 caactagaat atgtaagaaa ctaggtacag tatttaaaaa tagaggagct aaattagata
    37081 aaagtttata tattttaaac agttctaaac ccactgcaat actaattgaa agtttctttt
    37141 gtgataacaa ggaggattat gagaaggcta agaaacttgg atatgaaggt atggctaagt
    37201 taattgtaga aggtgtatta aataagagta ttgaaagtaa tgaggtgaag caaatgtata
    37261 aacacacaat agtgtacagt ggtgatgata gagtatcagc agatatttta ggattatatt
    37321 ataagagagc aaaagaaagt tatctagtta tagatataaa agactataaa ccacatagaa
    37381 cacaaaatct atatgtaatt ggtggagtaa cttgtaataa aatgaaagaa atgagtaaga
    37441 ctacaggaga aaaatttact caactatatg gtaatgatgt atggtcaaca atggataaag
    37501 ttatagaatt tgtaaaagaa aaattataaa ttaaaagaca acctttaaaa attaattgtt
    37561 aataaaacta ataaaatttt caactcagaa ggagcattat atgttaacat ttttagattt
    37621 gtttgctggc attggaggtt ttaggctagc aatggaaaga gcaggacaca aatgcgtagg
    37681 ccattgcgaa tacgataaat ttgcaaactt aagttacaaa gcaatgcata acccaaagga
    37741 ggatgaatgg tttgaaaaag acattagaga aattagagca gaaaatattc ccaaagcaga
    37801 tgtgtggtgt tttggattcc catgtcaaga cattagcatt gcagggaaca aacttggatt
    37861 caaaggaaat cgttcaagtt tatttttctc agttacaaaa cttattagag aacttaaaga
    37921 agaagataga cccaagtact tacttattga gaacgttaaa aatctactta gtgttaatag
    37981 aggatttgac ttcgccaaac ttctcattga gttggatgaa atcggctaca atgcagaatg
    38041 gcaaaatctc aattctaaaa acttcagagt tccccaaaac agagacagaa tatacattgt
    38101 tggacatctt agaagaagaa gtacaagaga agtatttcct atcacaggaa cgagtagaaa
    38161 aactactctt gagcaattaa ataatcctaa tcacagttca aacaggattt ataattcaaa
    38221 tggattatca aagtgtttat taagtaaagg tgggaacaat acaggtcttt atagagttgg
    38281 caatatcaat cctagtggaa atggtgcagg cggacaagtg tatagtagcg aaggcttttc
    38341 tcccacactt acaactggaa aaggtgaaag cccaaagata ttaattaaag agaatactaa
    38401 aaaaggatac atcaaagcat ctataggaga tgggataaga ctagatcata tagaaggtaa
    38461 aactggccgt ggaagagttc agcacaaaaa aagttgtact ttgactactg caagtaatgc
    38521 tggcgtaatt ggtaaaaaat ttagagtaag gaggttgact ccaaaagaat gttttaggtt
    38581 acaaggattt ccagacgatt attacgatag agcggcaagt gtctgttcag aaactcaact
    38641 atacaagcaa gcaggcaata gtgttactgt agatgttgtt gaagaaatag caaagaaatt
    38701 aaaatagaat attaatacaa tgcaaaatgt ggtaggtttt tccttccgca ttttgcattg
    38761 cattagcttt tttggaataa cttacctgta ctatcttaaa taaaaaaatg ctactatatt
    38821 ataaacattt acaaggagtg cataatgtta atacaaagct taaaacgtgg cgaattaatt
    38881 gtaatgaaaa tcttttagaa aagaaattca agaatatcaa agaaagaaat tattcgcatt
    38941 tgcaatgaaa aacacaattg gcggaaatct gcgacaaaaa ctctacttaa aagacttgtt
    39001 aaaaaaagag ttctacttaa gagaattaaa attttccaca tatattacat acctatagtt
    39061 acagaaaaag aatatttcaa atataaaata aatgatttag agccagatta tttagatgaa
    39121 tattttatac gattaatgtc cactgtacat aaatttaaaa atctagatga agaagattaa
    39181 atatctccct tcatctactc ttcacatatt ttaatagact ttcaagcaat aattgactta
    39241 acaaatcatg cttatcataa tgtttattct catctgcaaa tttattaaac atttcccaca
    39301 ctgtattatt tattctaact gtagttcttt ttatagattg atctggaaga tctatagata
    39361 tatcattttc tttaatgaca catatatgat aactatcatc tttagtttta aaccattcca
    39421 gcacattctt tatattatca atttcactag ataaatattt aatatcgtca tcaatcttac
    39481 tgctaatgct atctatatga tatttatcat cataactttt attatcttct acaaacttgt
    39541 ttccagttct tttatatcct ttcttattaa gccacttcct tatagtactt tcatttgccc
    39601 ctaattcatc tccaactttt ttaagacttt caaattcact taatttatta ttaatacaat
    39661 caacaacttc attaatattt ttttctttca aatcactcca agtcatataa atcctcctca
    39721 aaaaaataat agattacaaa tatcatatat ttgtaatcta ttatacatac tataaactaa
    39781 atcctttttt atttcatcat ttttttgtat ccttttgcat ttgcaaaaac atgtttatct
    39841 acatcactca taactattat gccaggtata ttctttttaa atgcttcacc taacaattta
    39901 gcgcctccac ccataacaac aaccgcagct gtactaactg gataatttaa tttcaattca
    39961 ttgtatattt ctttaaatag agaaatagat gcatctatat ttttctttat atcttgtttt
    40021 tcaccattta ggtaaaatcc tttatttatg tagtcttgta ttttttctct ttttatctca
    40081 actttaccta aaaatctttc gtccatctca agagatttct ttattgcatc atatatattt
    40141 attgttccca catttataga atatggatca gtcgctttat tcccttgtac aagcgcaata
    40201 tctgttgtct ttccaccaat atcaactaaa acaacatctc ttcctgcaat ttggtcaact
    40261 atgtcttttg cataatataa gtatcctgta acaccttctg gaaatgtttg aatagatctt
    40321 attgttatat ctcttttagt agtctcattt cctcttgtaa actcaacatg atattctttt
    40381 ccttgtaatt tttcaataag attttttctt aatccggcaa attgtttcac tggcagacct
    40441 agccctagat caacaatttc atcatctgta tttctacata tagcccctaa tagtagtggt
    40501 aagaagtttt ctttctcata tttaactata tttatttcct cgtcaccttc acctaggtaa
    40561 tatgttttat tgcctatttt aatgctatct gaatctgaac caaagttttt tacctctgta
    40621 actttacttt caaaaatatc atctgtactt gttttaactg cataatttcc aatatctata
    40681 cctaatttac tcatttatgt tatttccccc ttattattct aaacttccaa aatcaaaatt
    40741 gttagctgga tcttctattt cttttttctg ttcaactaca gcattatcct ttttttcttg
    40801 cattttttcc caaacaagct cttttaagta agctgtagct gataatttct catctaaaaa
    40861 cttttcaatt gctttttctt tatcattgtt tttaaatgtt agaactatcc tactcattca
    40921 accacttcct tttttataaa atattataaa atttattaat atttatatat attttactaa
    40981 attttacaca gtaattcaat agatttataa gatatttttt ataatatttt ataatttttt
    41041 acgtaatttt ataatttttt ataaaattta tcattttgta tataattaaa aacacacttt
    41101 ttataatttt gcataaaaaa agagtgtttc atatatttct aaacactctt ttctactttt
    41161 tataattatc taattgtaaa agaagcttca gctggactag agaataatat aatacctcct
    41221 gtacacacac cttttccttt aaatttagca atatatgaac ccttttttag ccctttaaaa
    41281 gttactacac cagttttagt agttccagct aatctaaagc ctacatactg agatgataca
    41341 attttgccag ccttatatac atctatattt ccttcaaatc ctaaaatatt tccaagcgta
    41401 gatgttactc tccaagataa ctgcccacca ctcattgcag ttaaattagc agtaccacct
    41461 ggaacaacaa cagatttagt cgaaggcgaa tctgagtttg gttggccaat taattcaact
    41521 gtttgtgaat ttccagtacc ttcataatca tttccatcag caaaagctgt aaatggaatc
    41581 attactgcgc aaagcgttag taataaagca attgtttttt tcatttgttt tccccctatt
    41641 tttttatatt attctcaatt acagtttcct ttctttacgt gccttaataa tcatattgta
    41701 attatgctca gcagtttcaa atgctcctac caaaggagtt ataattacac tttctattaa
    41761 gcacaattca tcatagttta aactagcttc tccagcatag aaattattta acttatctaa
    41821 atctattcta gttaaaacgg aaactgattc cattgagaat ttgtaaacat caataagttt
    41881 atataatcca cctatatagt tttttgctac ttcatggctc ttaaaaaatt catcttttcc
    41941 tgtatgtagg tttacttcca taattccctc cattgttctt atatttttaa ccttcaaata
    42001 atatttactt cttcttcaaa acagaatatt ctatcacggt agaatattct gtttttttca
    42061 ttctatcatg atagaataat attttcaatc attttttcta ttctatttta tttaaataga
    42121 atagaaaaaa agaacactta atatttttca agctaatata ttaagtgttc tttttatatt
    42181 tttacaagat tatataaaat tattctttaa tcaattaaag tgtttttcta tttcttaata
    42241 ataagaaatg atataaaatt gtatcatttt aaagattttg tgatataata aaagcaagaa
    42301 gaactacaat ctatttaggc gtagagtgga gttcataata aacgtagttt attttttgaa
    42361 tttaaatata aatttaaatt caacctgtaa gtcactcttt gcacgagagt ggctttttgc
    42421 ttttttgaat agttcactga ttaagtaaac taccacacta gctgttaaac tagctaaaac
    42481 attaaacaaa aagttatcca tgtaactcac ctcccctcga atcgttggga ggataatctt
    42541 ttgtacatga actccactct atagattgta tattacgctc ttcttgctaa atttaattat
    42601 accatatttt gtaatatcag ttaagagttt gcactacaat aatcagaaag cttgttccac
    42661 tcacaatcaa cccctattat tttaagaata tcattgactt ggctatttaa atctttaata
    42721 tctgatttag acattaaagg atctttcatt atttcattta gaaaaatact atcaatgatt
    42781 aaacatacat tttctgataa atattcacgt tttactgaga ttactgttcc aactgtactt
    42841 tcttttagtg tcttatatgc tacactcaat atgcatggtt ctaaattaat tccttctagt
    42901 actgtttcaa agtaatattt tattcttata ttttcatttc caataaatat ttcttcattg
    42961 ctgcttatct tctttaattt tctcattatg cctaacatct tagcattcac ttcatagttt
    43021 attccacatc tctttgatgc taaactcgac atcaagtctc tttctgaata aattacccat
    43081 tgaccattat aggaaaatga tgttttagcg atatccattt ctccttttcc agtaatatta
    43141 aatttaggct ctagtttatt tattaagtaa atctcataaa aatccatagt atatttatta
    43201 tctaattcta tatactcaaa tcttaaattc ttattacacc aattttcttt tttattactt
    43261 aaatgactag cgtgtctatc gcagatattc tctgttttcc caacgtataa acattcttcc
    43321 gtatctagat taatatattt ataaacataa tacatttttt attttctcca attattattt
    43381 atagtaatat ccttcttcat ttactagacc ttgatcaaat aaatttctcc taacagtttc
    43441 aagtctgaca ggatctttgc tataatcaac ttctctttct tcttttttaa catcttcatc
    43501 atcccaatac ttatccaatt cttctttaaa ctttaaatca tctttttgat gaattataac
    43561 ttcacttatt ttatttgaaa gtgttgattt aaaaaaatca taattctttg tatttataat
    43621 atcagtatta tctctttcaa atgctatagc aatagactta ttaaaagcat tcagataaac
    43681 atctttctta aaatcaaact ctttaaaatc ttctttaaat agtttaatcg tccctgttgt
    43741 aaaaatattt tcatttggta tataaaaact aaaagacact tcattgtcct gcttcttttt
    43801 aggtatataa tcttcaacca atatatcttt atcaaaatat tttctaggtt cataatctat
    43861 cacagaaaaa attatttcat ctatttttct attttttctt ttttcttctt ttattgaaac
    43921 aaccatgttg ccttttttat ttatttcatt cattgctctt tttagaacat tttgtttaaa
    43981 atatttatac tctggatata cattatcttt taatttcaaa taaaatctta attcatctaa
    44041 cttatatttt atctccactt ccttattttc tctgctccat agtctaaata aagtgtataa
    44101 cctctgagta tatgcacctc taaaattaaa taacactgat aaattaatag ctgtatatcc
    44161 atttttcttt ttctgcattt ttacaaaatc agttatatga ttgtatagaa cttcatgcat
    44221 cataattgta taaatttggt ctgtatgatc caattcatat gtattaatta aaccacttcc
    44281 aaaagttttt aacttgccag ttgtctcttc tatatagtca aattccaaaa cactttgttg
    44341 aaacatgttc aaaatttctt ttatattttt atgctcataa tcattattgt ttttcataaa
    44401 tacttttagt tcttcttttg atatagttgt aacataagat gaattttgct gtttctgagc
    44461 attaaaaagt attttgtaaa ataatttatt ttccacattt gtaaaatcat atttactttt
    44521 tattaaatta tttggttgca ttaaaatttc ttttttttcc aaaatctatc acctcatata
    44581 aattataata cctaatttat aaaaaggtca accatttttg aaagacgctc acctttgacc
    44641 cttaaaatga tgacctttga cccttaaaat gatgaccttt gacccttaaa atgatgacct
    44701 ttcgaccctt aaaatgatga cctttgaccc ttaaaatgat gacctttgac ccttaaaatg
    44761 atgacctttc atctctgaaa cctagatata ccaattatta tagcttgtct aaaaactatt
    44821 aaagacttta aaaactgttt taaaaactat taaagactag aaaagatgtt ctgaactatt
    44881 ctttttttat attaaaaaac tttagtattt atttttctag tatactttaa acacttacta
    44941 aaaataattt cttataaaag attggagatg aagaattttt tatgcttgaa aacagaaaac
    45001 aaattcagaa agaaatttac ttaccaatag caacatattg tttaaaagat aagcaatatt
    45061 atataaaaga atatggagca ctattaataa agagtgctga aaatagtgac atttttaata
    45121 cccataaaaa tagaattttg tataagaata aacttgatat tcaagaaata gcaaatgaac
    45181 tgaatgtaaa taaagcaaca atccaaagaa atataaaaaa actagagaag ttagatttta
    45241 aaattctaaa aatagaaaat accctaaatg gaattgtata ttgtttacca tcagaaaata
    45301 atattgattt aaacaaattt gcattgataa attatgaaat gcttaaaaaa atggtcaata
    45361 aatttaaatc aaatacaata aaagtatatt ttttacttaa aacaatcact acagaaacta
    45421 attttaaacc tgcaactaat agttttatag cagaaaatat aggactaagt tctaaaagta
    45481 aaaataattt agatatcata acttcttctg ttaaaacttt agaagaaaat aaatacattg
    45541 aaacaaaaaa agtaaatgta tatgaatatg acaaagaaaa acttagagaa gttccaaaaa
    45601 taagaaaagt ttatcggatt tgtaattttg atgagtggaa aaaggaaatt gataaaaata
    45661 aagtttatta aatatagctt aaaagcattt aaaggcttgt ctgacgtgtt ttaattacaa
    45721 acttgatgtt atttacctaa aaagatacaa atttgcttaa aaatggaaat agaaggtcaa
    45781 atatataaat agagaaccaa ataaaattat attggctctc tatttatctt cttcccaatc
    45841 ctcaataaac ttttctaaac tttttaattt attttcagac acttcttcat catcatgcag
    45901 agcacttata aaactttcaa aagaattttt gtgaaagaaa ctaaagaaac ttttagtctc
    45961 aacttttaaa tattcttctt tttctattaa tgctttataa tatgtaaatc tatctttctt
    46021 aacaacctct aaaaatcctc taccaactaa tctcctcaag aaagttaaca tagttgattt
    46081 tttccattcg tacttttcgc ctagtgtctt caaaatttca gtagatgcaa cttctttatc
    46141 ttcgctgtcc caaataaaca tcataaccaa caattcactt tctggaatct tttttatctt
    46201 cataaagtac accaccttct aaaagtatat ctaaaatact ataacaatag agttaactac
    46261 acaatactaa atgtctaaaa atgttttacc aatttttacc aattaatttt tcttt
//
LOCUS       DAYASV010000017        43403 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYASV010000017 DAYASV010000000
VERSION     DAYASV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43403
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.017"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(127..1557)
                     /gene="dcm"
                     /locus_tag="ACGI68_000988"
     CDS             complement(127..1557)
                     /gene="dcm"
                     /locus_tag="ACGI68_000988"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFH0137436.1"
                     /translation="MKKITIDKNRVRKIMKEKNIRSQNQLALNMGITKNQLSAILSEK
                     FTPIKSNVEKLCEVLDIGFDEIMCLEDVNEKQLKFTNICSSNKDYIEHRDCTYKELSE
                     YELNPRERNKNNKHIDISNVLAKRAYTCVELFAGAGGLALGLEKAGFKEVGLVEWDKY
                     ACDTLKLNRPDWNVIQGDIVKIAENGIKNYIDNNIEIDLLSGGYPCQAFSYAGKKLGL
                     EDVRGTLFYSYAKILEELKPKVFLAENVRGLVSHDGGKTLKTMIDVFSEIGYNVKYEI
                     LKAVNYGVAQKRERVIIIGTRKDLSHVDFKFPKSFEYVATLRDALKNVPKSEGARYPE
                     RKKKVLDMVPPGGCWIDLPDEVARDYMGKSYYSGGGKRGMARRISWDEPCLTLTCSPA
                     QKQTERCHPDETRPFTTREYARIQSFPDEWEFTGSVSQVYKQIGNAVPVKLGKAIGLS
                     IVDYLNKIEKIYVTEEVAITEEEYVI"
     gene            1770..2417
                     /locus_tag="ACGI68_000989"
     CDS             1770..2417
                     /locus_tag="ACGI68_000989"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HFH0137437.1"
                     /translation="MTELEKQEILDKAKDFFTNILVANHKKNTIKLNLKAFNINPFLV
                     KYLASFLTGSNKPEYIAKALIYPRVLGTSINTSFGTNLQKFCITTLDGFASTTSGIDI
                     EFIDCIDGRRKFCQIKAGPNTINKDDVKTIIDHFSSVKRLARTNGLSLSYNDLIVGVF
                     YGTENELNAHYKRINEEYPVVVGQDFWYRLTGDENFYFDLIDTIAQSALEEDCTV"
     gene            complement(2596..3786)
                     /locus_tag="ACGI68_000990"
     CDS             complement(2596..3786)
                     /locus_tag="ACGI68_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0137438.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYSWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLNDGLDTVGGKMTVCQLYEKKNSQRKNIKRA
                     TEKGRQYLMNALKNDLLGMRAIDTVKQSDAKEWAIRMSEKGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEQKVILTPEQEKHLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLKDSEMGYYIETPKTKNGKRELPLTE
                     RAYQAIQRILENRGKAQPLIVGGYSNFLFLNREGLPKVAGNYEGMVRGLIKKYNKYHT
                     DKLPNITPHSFRHTYCTNMANRGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(3865..4068)
                     /locus_tag="ACGI68_000991"
     CDS             complement(3865..4068)
                     /locus_tag="ACGI68_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0137439.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(4444..4692)
                     /locus_tag="ACGI68_000992"
     CDS             complement(4444..4692)
                     /locus_tag="ACGI68_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137440.1"
                     /translation="MKHIFKKPSFYLISSATDGDEKAIEKILAIYDPYISKCCLRSLY
                     DEYGNVYIAVDMELKGRIREALIKMILDFDIPLETEEE"
     gene            complement(4696..5115)
                     /locus_tag="ACGI68_000993"
     CDS             complement(4696..5115)
                     /locus_tag="ACGI68_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFH0137441.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVTKGIVRNYQKELKRRKNKEIPFC
                     ELPELVIEKLSVLDDYDSDYTLFNVCGNDISILDDELAEALKKLSDRNRENLLMYYFL
                     EMSDTEIAKLQNISRSGVFQNRYNSLELMKKILKGEK"
     gene            complement(5476..6840)
                     /locus_tag="ACGI68_000994"
     CDS             complement(5476..6840)
                     /locus_tag="ACGI68_000994"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0137442.1"
                     /translation="MGIKMKTSLTRIFYKFLVCLLGGLCLSVVVPFACMMFASSIGLV
                     TLADNNERQTKALVPILTATPNVSDIHFPTGTKFLRLTKNYGYIDTNMSEEEKEKALM
                     FAKTGFMDTSGKTQFIFITRDDEYIVLQYVIGSQYLNAQFNQYLPSPEIMMISLITIS
                     SLGVCVYLTVHFAKELRKELTPILEATKEIENNNLDFTIGHSKILEFENVIISFDSMR
                     NSLKGSLEKQWRSEKAQREQIASLAHDLKTPLTVMQGNIDLLDETELDEEQKLYLSYA
                     MSSSEQMKQYIKILIDISKASAGYQLQMEDVNFPEFWKHVLSQTEIICKDKGIVIQQT
                     QHNFPQNITGDRMLLERALMNLVSNSLEYSPENSTLYIDVDSKDNYLNICITDCGCGF
                     SQEALDHAKERFYMADQSRSSKLHYGMGLYIVDIIIKQHKGKLLLDNSTETKGAKVTV
                     KLPL"
     gene            complement(6831..7490)
                     /locus_tag="ACGI68_000995"
     CDS             complement(6831..7490)
                     /locus_tag="ACGI68_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137443.1"
                     /translation="MATILMIDDERPILELVKNGLQKDGHLVTVYTSAAQVPLDKLNR
                     YDLIILDIMMPDIDGFSYCDKIRSLVDCPILFLTAKTMENDITFGLGLGADDYLTKPF
                     RITELRARVNAHLRREHRERHIALTFDRIKIDLSAKELRVDNIPVPLTKSEYLICEYL
                     ASNKGQVFSKEQIYEATFSLYGESDSSTISTHIKNIRSKFEKTGVQPIVTVWGIGYRW
                     E"
     gene            complement(7553..8362)
                     /locus_tag="ACGI68_000996"
     CDS             complement(7553..8362)
                     /locus_tag="ACGI68_000996"
                     /inference="COORDINATES: protein motif:HMM:NF033433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NisI/SpaI family lantibiotic immunity
                     lipoprotein"
                     /protein_id="HFH0137444.1"
                     /translation="MSKKMMILLFAVIICLSLAGCSLQDKIGDYSREQCYLNKENVTQ
                     FSYKGNDYTILADTVSNSGLGEWIGYIRQLAAVDESGKILLQENLKTATFQTLADLAD
                     LVDKAPNDAYIIPFLNVYAAPNADDYLIVDINGGYHKAVIDENIKGTDTVFDFKDIEQ
                     SMSGKFEINPQNATQLLCDGTVYQVTSDTVSNNELGSYIGILAENVIFNAETKIPLSK
                     EELRKIDWYGENAGQHREQWIYKDIYEIHGTEKTEAVAVQINDRYYIAKRQ"
     gene            complement(8355..9098)
                     /locus_tag="ACGI68_000997"
     CDS             complement(8355..9098)
                     /locus_tag="ACGI68_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFH0137445.1"
                     /translation="MFSIIRCFQAEWLKSRHTPLLLVHVLFPLIGAGVFAGYFHISGW
                     SEMTNVTTFLEVLAIIFPFVIGIVVGMIVELENGAGHFQLILGTIRSRIAVYIGKLVY
                     LIVLATFATILCIFLFAVLYPVMPFSFYIKPLIMLILSVIPLYLISIPVGFSLGKSVS
                     MGIGIVGSLLSALLVTGLGDFIWKFLPWGWGVRFMDYCVLRYVNEGQFHSNLPEFQLG
                     FILMLVFTVVLFIISLVWFQRWEGVKENE"
     gene            complement(9098..9841)
                     /locus_tag="ACGI68_000998"
     CDS             complement(9098..9841)
                     /locus_tag="ACGI68_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFH0137446.1"
                     /translation="MKNYISSEYLKHKNTFAKKLVWLAPILTLCLNILAPMWYQQNSY
                     NWWYVFLYPGCLTLLSILIIQRDNGKLKFRAILSLPVDLKKVWYAKIITCIIYIVLAN
                     TILMLCNVAGGFIIQHIFDIPMTISPMQALFGTLCIILASIWNIPLCLWFTKKVGVFA
                     TLILNVGMSFMLGTLGANTSFWFICPYSWVTRLMVPTLGILPNGESVTGSALSTPLPL
                     VIMTICLSLLLLYTISKSTAKSFIKQEVL"
     gene            complement(9843..10544)
                     /locus_tag="ACGI68_000999"
     CDS             complement(9843..10544)
                     /locus_tag="ACGI68_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0137447.1"
                     /translation="MKAILKTNNLCKDFKKQKAVNNVSITVRENSIYGLLGPNGAGKS
                     TTLKMITGMLRPTSGKVLFNGHEWNRKDLEQIGALIETPPLYENLSAVENLEVRAKLL
                     NIPKTRIDKVLKMVDLQNTGRKKAGQFSMGMKQRLGIAIALLNSPKLLILDEPANGLD
                     PIGIQELRSLIRSFPSKGITVILSSHILSEVQLIADDIGIISNGILGYEGQMNKDENL
                     ENLFVEVVRKSQEER"
     gene            10812..11063
                     /locus_tag="ACGI68_001000"
     CDS             10812..11063
                     /locus_tag="ACGI68_001000"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137448.1"
                     /translation="MKLFIGDIIQKLRKIKGLTQEQVANALGITTSSVSKWETNNTYP
                     DISLLSPLARLLGTAVDNVFNFKQKLSEEEIDTLLNRSK"
     gene            complement(11299..11466)
                     /locus_tag="ACGI68_001001"
     CDS             complement(11299..11466)
                     /locus_tag="ACGI68_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFH0137449.1"
                     /translation="MMPICNNKTRTKIRVDTELISFPLFCPKCKHETLINIKHGNVTT
                     IKEPDAKTQSR"
     gene            complement(11500..12114)
                     /locus_tag="ACGI68_001002"
     CDS             complement(11500..12114)
                     /locus_tag="ACGI68_001002"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFH0137450.1"
                     /translation="MKKVYVYLLDDMAECEIGYILQAFSMEKLLKNGTKEFEVKTVGF
                     NKEPVLTLGGLTIVPDYSISEVDFNASTALLLPGSSTWGSKENQEILEKAQECLNKNI
                     LVGAICGATLALADYGILDKFKHTSNSLEYLNFFSRNYAGQSLYVCSNSVLDRNLVTA
                     NSAGSLEWTKNILNYLNVYSDNKITAFYNYYSTGNAKYYDELLK"
     gene            complement(12185..13099)
                     /locus_tag="ACGI68_001003"
     CDS             complement(12185..13099)
                     /locus_tag="ACGI68_001003"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137451.1"
                     /translation="MKIERLIGIIFYLINRECVTTNELAQKFEVSRRTILRDIDTLTL
                     AGIPIYSELGVNGGYIINKDFKVDEKIIDSNNKEYILLALNSLRTVYGNKKIIETYEK
                     MKHLYNDVNINALPDVDFSVVTENPQIIRCVDLINKATKEQRTIKFIYTNSAGHTKKV
                     FLNPLHTYYRWYSWYVFGYDTSKNDYRMFKVVRMKNISLTKELFQTKDNIADLLSDFE
                     KRRNQTNITVTLSYKQEIDTLVNEYFKGKIVETVNDCFIREIEIKENDFMTFSILLGL
                     CDKVKILSPQKYKEKVLTHIRKIKKNFT"
     gene            complement(13096..13323)
                     /locus_tag="ACGI68_001004"
     CDS             complement(13096..13323)
                     /locus_tag="ACGI68_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137452.1"
                     /translation="MIGLKKRDIKKALKAGAKAGGMSLADVRADIEATIDEAMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYIITVKGLKV"
     gene            complement(13343..13960)
                     /locus_tag="ACGI68_001005"
     CDS             complement(13343..13960)
                     /locus_tag="ACGI68_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFH0137453.1"
                     /translation="MKNSISLFLPLALLLDLTIPFFLAPFYKGYSHSMQVMSVLGNNK
                     SPLHFIYNLWLVLCGIILLMSGFKVYEIVSQKSKNIAVILFIVILIYAIGGCILSGIF
                     SVGETKSLNTIPEKIHGYGAVLGFICLTFAPLLISLYWFRIEEIPLALFSMICFLLAF
                     IFFTIFVMADKPNYKDTIIAFEGLWQRLSLLFMYLPLGCLILLEK"
     gene            complement(13950..14183)
                     /locus_tag="ACGI68_001006"
     CDS             complement(13950..14183)
                     /locus_tag="ACGI68_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008791134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0137454.1"
                     /translation="MKKGQHIFGTVKVGERGQIVIPKEAREIFDINAGDTLLVLGDEE
                     QGIAIVKADVMKELAVKILKGLGSLSGGFNDEK"
     gene            complement(14328..15230)
                     /locus_tag="ACGI68_001007"
     CDS             complement(14328..15230)
                     /locus_tag="ACGI68_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFH0137455.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHQKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEIIELRLQDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAING
                     YLTKELQDLNVDTIRTDIPTSSTVTDVIVWNIEQSGTDTFSVTYEVDQQIKEGEQTTA
                     IKATYTVKVHVDADGDMVIVQNPTLAPAIEKSDYEPKTPEADASVDADTVNDATSFLE
                     TFFKLYPTATEKELAYYVSGNVLEPIGRDYFYSELVNPIFTKDGDNVKVKVAVKFLDN
                     QTKATQVSQYELVLHKYNNWKIVG"
     gene            complement(15247..16254)
                     /locus_tag="ACGI68_001008"
     CDS             complement(15247..16254)
                     /locus_tag="ACGI68_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFH0137456.1"
                     /translation="MKLKHIALIGSLFPILFSLVLFFGVLISADSDDENSNFSSSITG
                     MNLSAEVLKHQPMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGMDDLNVAIQSYNYGGGYVGYVAGNGKKHTYNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYQYGNQFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGTYVTHVAIYVGNNKIYHAGDPIGYADLNNSYWQQHLIGAGRVK
                     Q"
     gene            complement(16251..18461)
                     /locus_tag="ACGI68_001009"
     CDS             complement(16251..18461)
                     /locus_tag="ACGI68_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFH0137457.1"
                     /translation="MKERLKGLLTKRKLFHFVKVAVLAIFISIVFLSLLGTVAHATGL
                     VDDTVNTENLYSKYPLSNYQLDFYVDNSWGWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLSGVTENGFSSTGFYIGFLLLIIL
                     VVGIYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSESKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQRKKNIADKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTIPKPSAEKMQEVKRPATATTSKASEPVKTNVIKERPLSSGASDRKATQSAQP
                     VHRQNVEKVVSQETRQNDTKDRRIKVQQTQSVQKNQQTTEKSRNLVTKKGQKKK"
     gene            complement(18461..20911)
                     /locus_tag="ACGI68_001010"
     CDS             complement(18461..20911)
                     /locus_tag="ACGI68_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137458.1"
                     /translation="MFPIKYINNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATERSIRSMQEQSKKLVTGKLKEVAYQKIDEQTEALVSMI
                     GENQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGVAYEDYEYQLPKKNYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFIFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGNETISNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGETTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPNKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(20889..21287)
                     /locus_tag="ACGI68_001011"
     CDS             complement(20889..21287)
                     /locus_tag="ACGI68_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFH0137459.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFLI
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(21405..21908)
                     /locus_tag="ACGI68_001012"
     CDS             complement(21405..21908)
                     /locus_tag="ACGI68_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFH0137460.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPISIGEYVSIEELNSMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIETSR
                     GMCEIPY"
     gene            complement(21926..22429)
                     /locus_tag="ACGI68_001013"
     CDS             complement(21926..22429)
                     /locus_tag="ACGI68_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFH0137461.1"
                     /translation="MQETRVLVETRGTTGKETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEQTSVYQLNELDFMYRQLPADMQEEYIALLTVFENLEALYICR
                     NVITVYPDCKSMIDVAKQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENRRYLVTEH
                     SVFELPE"
     gene            complement(22348..22644)
                     /locus_tag="ACGI68_001014"
     CDS             complement(22348..22644)
                     /locus_tag="ACGI68_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFH0137462.1"
                     /translation="MSDIFKDMQANVGCDYISNLPSYKRKVWHEMKRLPLTNYDIKQL
                     EDFSKYVFGMSYQSLKDVMKQQKGREEQCRKQGCWWKREEQLAKKQHHTGSTCR"
     gene            complement(22733..23374)
                     /locus_tag="ACGI68_001015"
     CDS             complement(22733..23374)
                     /locus_tag="ACGI68_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0137463.1"
                     /translation="MWIYKILIYGGILLKKAMIYVHGKGGSYLEAEQFKNICLDFDVF
                     GVDYNDYFPWIVKNEIMEVYDKISEKYDYIYVLANSIGAYFTMHTLQNFKIEKALFIS
                     PILDMEQLIVDMMSWANVSEKELSERKEIPTDFGETLSWEYLCFVRENPINWKTPTEI
                     LYAEHDNLTSRQTVDKFIDRHCANLTVMNDGEHWFHTNEQIFFRDNWLRKVIQ"
     gene            complement(23409..23630)
                     /locus_tag="ACGI68_001016"
     CDS             complement(23409..23630)
                     /locus_tag="ACGI68_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137464.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMSIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(23632..23766)
                     /locus_tag="ACGI68_001017"
     CDS             complement(23632..23766)
                     /locus_tag="ACGI68_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFH0137465.1"
                     /translation="MWELLKDFLLVSVGTGIGVALMCILSVGKEADYEMKKLKESEDK
                     "
     gene            complement(23780..24976)
                     /gene="mobT"
                     /locus_tag="ACGI68_001018"
     CDS             complement(23780..24976)
                     /gene="mobT"
                     /locus_tag="ACGI68_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFH0137466.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASDITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LVNGGVMKRIDLAINDHTGILDIPELAEKCRKREYIGKFRSYKFYQSGELIKHREDDR
                     EYMGRTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEKAEIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFVDEEPDKRKNDWKLNERWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKRIDKGNGTDYMKTIEQQAKLTEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(25159..26553)
                     /locus_tag="ACGI68_001019"
     CDS             complement(25159..26553)
                     /locus_tag="ACGI68_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFH0137467.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAILYILDPKNADLADLGTVMENVY
                     HTKEEMIDCVNAFYEGMVQRNEEMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAQARQ
                     DGTATCEAKGDGTD"
     gene            complement(26647..27084)
                     /locus_tag="ACGI68_001020"
     CDS             complement(26647..27084)
                     /locus_tag="ACGI68_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137468.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYSHTEVRKLTSDEIKQYMSER"
     gene            complement(27186..27569)
                     /locus_tag="ACGI68_001021"
     CDS             complement(27186..27569)
                     /locus_tag="ACGI68_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFH0137469.1"
                     /translation="MRLSNGFVIDKERTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAEN
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(27585..27911)
                     /locus_tag="ACGI68_001022"
     CDS             complement(27585..27911)
                     /locus_tag="ACGI68_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFH0137470.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(28150..31194)
                     /locus_tag="ACGI68_001023"
     CDS             complement(28150..31194)
                     /locus_tag="ACGI68_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFH0137471.1"
                     /translation="MKKTLKRLCTGFLALATVVTALPTTPVHAESKQYWTESKERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSLEYVKQYGETHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFSGARAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNTKLQKVSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDENGNTDIVEVKAGTVYIKELSAPAGYKVDKT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKGNPQGNASLAGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAVLPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLANVPFRITSKTTGENHVVVTDDNGQFSTSAEWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEEMRCESNKGFELIPPFEIVVSRS
                     NLVVDLGTLTDEYEKEISIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFVADDEEMKVEISYTFNASALGGKNLVTFEELYD
                     FSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKKVTIIDTVT
                     LESLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKVEVAFTFDATSL
                     DGKQLVTFEELYDFSNPDEPKKVTEHKDIEDKGQTITFKEKPEEPEKPETPPTPEKPN
                     RPSDSPKTGDSTNVMAFVVMLLVSAGGLAGTYLYKRRKMKKS"
     gene            complement(31796..32074)
                     /locus_tag="ACGI68_001024"
     CDS             complement(31796..32074)
                     /locus_tag="ACGI68_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6809 family protein"
                     /protein_id="HFH0137472.1"
                     /translation="MYDEIIEIYFDYWINEHMYRKVLEKDEYYNDRCKEIKKVNKLLE
                     KNLSEEKKMDVLIELNDLFNSVIEIERELGYKLGFKAGARFMIELLNS"
     gene            complement(32322..33656)
                     /locus_tag="ACGI68_001025"
     CDS             complement(32322..33656)
                     /locus_tag="ACGI68_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0137473.1"
                     /translation="MDNMSNLVKNTEAEQSILGSILLDSRKIEEIEILISEDMFYHQE
                     HKHIFNSVKDIHKRDEPIDIITLTEDLKKKGLIDYVGGISYLTKLSTIVPTIGNVSHY
                     IEIVIEKYKNRNIIDKFNKFKLGEIDDNTLIYQLEKYVNEVNQKLVKKDSSITAISER
                     AFEYINDEIEEGIDIGIKFLDETIGGLYSGELITIAAKSGRGKTALALQIMRNVVFQG
                     KKVLFISGEMSDIQILFRNIASLTGISVVKMKNRNLNEEEIIKYINALSTLNQENNLY
                     INDSVNTISGIKREIKSIKPDVVIIDYLQILDYEGKEVGEAKFADLSRQIKKITLDFN
                     IPIIQLAQLNDELKDERPKGDRVMRSSKQIYMDSNSVIYIHRPTEKEALDYCKTMGIE
                     DIKELRRREKGFGANLSEIIVAKNRDGESGFRPYWYIGSLFSFKECKKVKEV"
     gene            complement(33669..34412)
                     /locus_tag="ACGI68_001026"
     CDS             complement(33669..34412)
                     /locus_tag="ACGI68_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137474.1"
                     /translation="MSNLGWIKLHRKIKESPLYKDCNSKQRDILINLLLSVNHKPNKW
                     VYGGKEYEVKSGQLITSLESIANMCSKDVSIQNVRTALKKFERYGFLTNKSTNKNRLI
                     TIENWELYQSNEENQQTNQHAINKQLTTNKNVNNEKSKIYSEIIEKFNITCTELSKVK
                     KLTEARKKVINARIKEYDKETVLEIIHIVSKTSFLNGSNEKGWKANFDWIMSPNNFIK
                     ILEGNYQDKSINVKVDAEAYSPDDSQLKW"
     gene            complement(34433..34747)
                     /locus_tag="ACGI68_001027"
     CDS             complement(34433..34747)
                     /locus_tag="ACGI68_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137475.1"
                     /translation="MADTNNLEVLKTLQATLNILVKQEQEAQEKEKGYKEIQTYRRDK
                     AQLITCKEASKIYPIGESKFRQLCHSKNKGFPCIWIDNRVYIIKNKLDNWFIENANGI
                     KL"
     gene            complement(34751..34945)
                     /locus_tag="ACGI68_001028"
     CDS             complement(34751..34945)
                     /locus_tag="ACGI68_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137476.1"
                     /translation="MKLKLKRIEKGIKQYELAEKVGISRYYLSSLERGKSKNPSNMLM
                     LKLAQELDTSVEELFFDKEE"
     gene            35085..35945
                     /locus_tag="ACGI68_001029"
     CDS             35085..35945
                     /locus_tag="ACGI68_001029"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137477.1"
                     /translation="MIDNTIGKKIKQYRKSINMSRDELANKIGCSVYAVAKYEQGQRK
                     PSLDMIKKISTALSIPINRLIIDESDLDTSTNNYTNLKELDLVVKTFDNYSAKLIGNN
                     ISKFRNINNISLKELSEKINCSENEIINYENGTKMPRIEILKKIALATYTKIEDLFGF
                     PLEEIKIDNQTILYRNPQTGYIIDNFLIDFINKDINIDNTPVSYYIDKSEFDLVIHIL
                     KNYGYDVKTDYKTKFEIADNADTIKPISLSFSDFKDFSKNLNWVINGLVENFIQENSN
                     GLIEDEDEEY"
     gene            36042..36794
                     /locus_tag="ACGI68_001030"
     CDS             36042..36794
                     /locus_tag="ACGI68_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0137478.1"
                     /translation="MNKSYIPKYTHKFPTNLPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVSDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILISNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDEYNYKSYKELFKNGN"
     gene            36784..37950
                     /locus_tag="ACGI68_001031"
     CDS             36784..37950
                     /locus_tag="ACGI68_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0137479.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPIRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKDLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQEPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNSDILKAGELYRNDNYVFCNELGAPIDDKRPLRNLKSILNSLDIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKIEAVEKLDKIFSL"
     gene            complement(38067..38297)
                     /locus_tag="ACGI68_001032"
     CDS             complement(38067..38297)
                     /locus_tag="ACGI68_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137480.1"
                     /translation="MEANKTLLQSLYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGISDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(38546..43117)
                     /locus_tag="ACGI68_001033"
     CDS             complement(38546..43117)
                     /locus_tag="ACGI68_001033"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0137481.1"
                     /translation="MTFEEIMEFNKPYNQKNFDELVNAIKDGYVVPYIGAGMSMLFKD
                     IYPSWSTFLNNTSEEYLEDEDKILFEKIEDYEKKAEFLYEELAKVRFSKHLKKVFGAG
                     YLDAKDKDDFIDKCVYLIPIIFGSGLIITTNYDKVLEKNYGLHNKTVLVSHPGHFEAL
                     NNALRNNELVLYKIHGDITEPTTSIVLTKEQYEKAYSNDELRKMLTQVYTSKELLFLG
                     CSLEKDRPLELLQQVSEAGTGHYAIIPVSSEKKKHRRIELENEYYIQTIMYPEGKHEC
                     VRIILERILEIVLPDEYRRLKDRSLKESRLTNLSCRLTEKWFQNQNNIQIKNLGDRYL
                     PELNVELSLENTFNALGKNEEFKQRFDEKTDKVLSSIKKQKINEVEVDVLVIEEIIQQ
                     FYDKANERLDIKLLKKHFEKISAALDDRINNFKNIIINEDCEKSKKREDEIYKLKQTQ
                     KDIYVYIKYLDSKEIKVVNSPYILLHSAGGMGKSHIIAHTIEKRNMDGHKSILFLGQH
                     FKLDKTPIKHMLEILEIECEFEELLEILNEIGEKEQQRVILFIDALNEGQGKELWKYY
                     LNGIVEQIKKYPWIGLILSIRSEYIPSLIDNNNKIKENLVFIEHQGFSSIEHKAMKKY
                     FDYYGIPYSNIPMPNQEFRNPLFLRIMCETFKGEEINLDTISFTDIYKNYLNILNLRI
                     SESCNYSRHANVVTEVLNEMIKYKYGDGLGNNLIPANKSVEIIINIASKYKINNVIID
                     ELIANGIVNKTVNLNDEEYMHVTYEKLEDYLYANLLVKELKEIGVVDFSHKYKYLVEY
                     EDILEVLAIVLSEETGSDIPNEIFEVFKNKETNYNVISAFINALKWRKKSTITEKTLD
                     YINKIVLGKSYYKIDFYNVLILISTKVNHTLNADYIVKYILQNTMADRDAIFIPIFNE
                     VYEEEESSIKRLLGWCFDFEGFDNTSNETIRLSAMMIATFLISSNRDLRDKSTKALVN
                     LLTGKMDIVIDLLKTFEDVDDPYIIERLYAVAFGCVVSEQSEEMIEALAVYVYNKIFD
                     VDYVYPNILLRDYAKNIVDYAKYKIDSSVLKDMNVEPPYKSIFPKVPTDEEIEGYKYD
                     YKASDFKDYYWSQNTILNSMEVEYTRDGQPGWYGDFGRYMFQHYFHKWKGLDYNDLKN
                     IAIKKVFDMGYDVNKHGKFDRTVQNGRLRDSKYERIGKKYQWIALYELAAQAADNYKI
                     EVYTDAYGTKKEIYCKGTFEPDIRNIDPTVMTVINNNNNKRVHNQLFQFSPIPNNEWL
                     GDFSDLPSINDMVNSVYKNQDFLLLNGWYTWKEENDFKNKNYKNPEKDMWVLINSYII
                     KNDQLEPIVNALKGKDFMGRWLTEPNSNYTLYNKEYYWADGYYFFQNSYYCGEEWVQI
                     DEHKNILEKDYKVLLPTYSYTTEREGDIFQEDFSGTWHKPCSTLFYGLKLRYGQGDSI
                     LYNDYGEVVCFESSELLNEEIGFFINKEIFLKYLNEQGYSVFWTMLAEKRVLEEPYMD
                     RKYIQPTISGVYTLNGENRLVGETIESEY"
BASE COUNT        12963 a         8643 c         6536 g        15261 t
ORIGIN      
        1 aaaattaaag agagcaattt tttttgctct ctttcaaaat ttatattaaa ttgtaataat
       61 aaaaataaaa acgctagtta tctaaggcta atattttcaa cgattatata aatatctaca
      121 taacagttaa attacatact cttcttcagt tattgcaact tcctcagtga catatatttt
      181 ttctatttta ttaagataat ctactatact taatccaata gcctttccta gcttaacagg
      241 aactgcattt cctatctgct tgtacacctg actaactgac cctgtaaact cccactcatc
      301 aggaaaactt tgaattctag catactctct agttgtgaat ggtctagtct catctggatg
      361 acacctttca gtttgctttt gtgcaggact acatgttaaa gttaggcatg gttcatccca
      421 acttattcgc cttgccatac ctctttttcc gccaccgcta taataacttt ttcccatata
      481 atctcttgcc acctcatctg gcaaatctat ccaacaccca cctggtggta ccatatctaa
      541 cacttttttc ttcctctcag gatatctagc tccttccgat tttggaacat ttttaagtgc
      601 atctcttaac gtagcaacat attcaaacga cttagggaac ttaaaatcca catgtgacaa
      661 atcttttctt gttcctatta taataactct ttccctcttt tgtgcaactc catagttcac
      721 agcctttagt atctcatatt ttacattgta tccaatctcg ctaaatacat ctatcatagt
      781 ctttaatgtt tttccaccat catgagatac aagacctcta acattctctg ctaaaaacac
      841 cttaggcttt aactcttcta gaattttagc atatgaataa aacaatgtcc ctcttacatc
      901 ctctaaccct agcttcttac ccgcatagct aaatgcttgg catggataac cacctgatag
      961 caaatctatc tctatattat tatcaatata attctttatt ccattttccg ctattttaac
     1021 aatgtcaccc tgtataacat tccagtctgg tctatttaat tttaatgtat cacaagcata
     1081 tttatcccat tctactaatc caacctcttt aaaaccagct ttttcaaggc ctaatgctaa
     1141 tcctcctgct cctgcaaata attcaacaca tgtatatgct cttttagcta gtacattact
     1201 tatatctata tgtttattat ttttatttct ctctctagga tttaattcat attcactgag
     1261 ttctttatat gtacagtctc tatgctctat ataatcttta ttactgctac aaatatttgt
     1321 aaatttcagt tgcttttcat ttacatcttc caaacacata atttcatcga atccaatatc
     1381 taacacttca cataattttt ctacattact ttttataggt gtaaattttt ctgataatat
     1441 tgcagataac tgattcttag ttatgcccat atttaatgcc agttgattct gacttcttat
     1501 atttttctct ttcataattt ttctcactct atttttatca atagttattt ttttcattta
     1561 gttcacctct taataaataa tataccctta aatagttaat aaaatacatt aactatttaa
     1621 ggatatatta tcataattct aatttttatg taagtagtta taaaaactta aaataaaata
     1681 tctgtaaaca acctaatatt acctcataat tttacacatt atggaatata ttgtataatt
     1741 acattaatac tttttagggg gattatttta tgactgaatt agaaaaacaa gaaatactag
     1801 ataaagccaa agattttttt acaaatattt tggttgctaa tcataaaaag aatactatta
     1861 agttaaattt aaaggcattt aatataaatc cttttttagt taaatactta gctagtttct
     1921 taacaggatc aaataaacct gaatatattg ctaaagcatt gatttatcca agagttctag
     1981 gcacttcgat taatacgtcc ttcggaacta atttacaaaa attctgtatt accactttag
     2041 atggttttgc ctctacaact tctggcattg atatagagtt tattgattgt attgatggta
     2101 gaaggaaatt ctgtcaaatt aaagctggac caaacactat taataaagac gatgttaaaa
     2161 ctataattga ccactttagc tcagttaaaa gacttgctag aacaaatgga ctttcattat
     2221 cttataatga cttaattgtt ggtgtttttt atggaacaga aaatgagctt aatgctcact
     2281 ataaaagaat taatgaagag tatccagtag ttgttggtca agatttttgg tataggctta
     2341 ctggtgatga aaatttttat tttgatttaa tagatacaat tgctcaatct gctttagaag
     2401 aggactgtac tgtttgaaat aaaaacttac taaatttctg attatctttt cataagttgg
     2461 taaatgccga tatatcccgt attttagggc tttatcggta ttttcttttt ggaagatacc
     2521 ttgcataagc tggcatttac cagcatgaaa atgcaaccaa aagtagtaaa tgagtagtaa
     2581 atataagctc cgatattaac aagccagcct ttccagttcc gcttttgcag attgaaatgt
     2641 accgtgtgcg taatagccaa gtgtcatggt tatgttagcg tgtcccatga gatattgcag
     2701 ggtgtttggg ttcattcctc tatttgccat attcgtacaa taagtatgtc tgaatgaatg
     2761 tggtgtgatg tttggtaact tatccgtgtg atacttgtta tatttcttaa tcaatcctcg
     2821 caccataccc tcatagtttc ctgcaacttt aggcaagccc tcacggttta agaataggaa
     2881 attgctgtaa ccacctacaa tcaacggttg tgcctttcct ctgttctcta gtattctttg
     2941 gattgcttga taagcccgtt ctgtcaatgg aagttcacgt tttccgtttt tagtctttgg
     3001 cgtttcaata tagtaaccca tttcgctatc tttcaataac tggtggtcta tattgagtat
     3061 tctgttctgc atatcaatat gcgtcgtcaa tccgcaaaat tcagaaatac gaagtcccgt
     3121 ttccagcaga agcacaacct catcataata cttactgtaa atcttgtccg tttccataaa
     3181 ggcaagcagg tgtttttcct gctctggtgt aaggataact ttctgctccg tatcatcttc
     3241 cagaacatca cttagcttaa attcaaacgg gttctttctg atacagtcgt cttgtatcgc
     3301 catataaaat gacgctttca aggaacgctt atagttatca atcgttttat aggcatatcc
     3361 tttttcactc attctgatag cccattcttt agcgtctgac tgtttgacgg tatcaatcgc
     3421 tctcataccc aacaggtcat tttttagagc gttcataaga tactgtcgtc ctttttcagt
     3481 agccctcttg atgttctttc tttgactgtt ctttttctcg tagagctggc agactgtcat
     3541 tttaccgccg actgtgtcga gaccgtcgtt aaggtctttt tttatctgtg cttctttttc
     3601 cctcaacgat atatcatcac gctttccagc aggtgtcttg tctgtcggta caagtttcca
     3661 agaatagata aactgtggct ttccgtatat atcggtatat ttataaacgt atcttccgtc
     3721 ttttcgctgg ctctctccat tacgcaaatt gcgattttta ctgtcttttc gttttacatt
     3781 agacatagtg taaagctcct ttccgtcatg gaatgagccg tgatacgcta taccttatta
     3841 taccatatct acggctcaat gacattagat ttcgtccaat gtatctatga ttttttcaaa
     3901 ttgttttcgc ttaatctgaa tacgattacc gttcacgata acccagccag cgtcaatatt
     3961 ttcttcggct aatttacgca atttcttttc gccaatgcgg aagtatttag acgcttcttc
     4021 aatcgtcagc gtatattttt cccagatagg cacatcagta ttgttcataa tctatgcacc
     4081 ccctttactg tgtgtcattt tttacaagca ggcagacggc tttggaaagc tccgttagta
     4141 tctcaactat tcccattctt gtgggcagaa ccgcaccatg cggacgtatc attattctgt
     4201 gaggataggt cgtggcaaaa cgacttttcc aacagtcgct ctcggatcac tgcgtgggtc
     4261 gctcgctttc ttttgaaaag gtcgtggcgt acagtccccg tggctcgcta tctcacaaac
     4321 gtatctgtct gctttattca gttgtcaaag agctgtggcg aaagcgtgtt gctttcatgt
     4381 aaaagcaggg agcagagcag gaaagagggg attgaacaaa tcatgctctg cggttactgc
     4441 ttgttattct tcctcggttt ctaaaggtat atcaaagtcc aaaatcattt tgataagggc
     4501 ttctctgata cgtcctttta attccatatc cacggcaata taaacattac cgtattcatc
     4561 atagagtgaa cgcaaacaac attttgatat gtacgggtca taaattgcca gtatcttttc
     4621 aatcgctttt tcgtctccgt ccgtagctga cgaaatcaga taaaatgacg gtttcttaaa
     4681 aatgtgcttc atttcttact tttctccttt tagtattttt ttcataagtt ccaatgagtt
     4741 ataccgattt tgaaatactc cacttctgga aatattctgc aatttagcaa tctcggtatc
     4801 gctcatttct aagaagtagt acatgagtaa gttctcacgg ttacggtcag acagcttttt
     4861 caaggcttct gccaattcat catcaagaat actaatatcg ttgccacata cattgaataa
     4921 tgtatagtcg ctgtcgtaat cgtctaaaac ggataatttt tcaatcacta gttctggaag
     4981 ttcacaaaaa ggaatttcct tattttttct ccgttttaat tctttctgat aatttcttac
     5041 aatgcctttt gtaactttct ttaacttaca ttcaaattga cattgtattg ttttctggaa
     5101 atcagacggc ttcataatct cacccccttt ccagctatga cttgaaaagt gtttatccct
     5161 ctttccgcac catatctgta cagcaaggtg tgatttgcta agctgaagcc gaaaaaagtt
     5221 agaaaatttt ttcgggtaca aaaaaagccc gcacaaaaca tatagtctgt acgagctaag
     5281 atattaaaat attcagttgt attagcgcat ggattgctgt taagttgttc ttgagttttt
     5341 ggtaaattgc ttaaaacact tcatacacaa tcccccgtat aatagcatat aaccattacc
     5401 ttcaaacaaa gaatacaacc taattttgaa gatttcttaa agatatgaaa aaagaacgac
     5461 aaataagccg ttctcttata atggtaactt tacagtaact ttggcacctt tggtttctgt
     5521 ggaattatct agcagaagtt ttcctttatg ctgttttata atgatatcaa caatatataa
     5581 acccattcca taatgcagtt tagagctcct actttggtct gccatataga aacgttcttt
     5641 tgcgtgatcc aatgcctctt gcgaaaagcc acagccacag tctgtaatac agatattcaa
     5701 gtaattgtcc ttgctatcta catctatata taatgtagag ttttcaggcg agtactctaa
     5761 agaatttgaa accaaattca tcaatgctcg ttccagtaac attctatccc cagttatatt
     5821 ctgtgggaaa ttatgctgtg tctgctgaat aacaatacct ttatctttgc agatgatttc
     5881 agtttgagat aagacatgtt tccaaaactc tggaaaattt acatcttcca tttgtaattg
     5941 atagccagca gatgctttag atatatcaat caaaattttt atatactgtt tcatctgctc
     6001 agagctactc attgcatacg ataaatatag cttttgttct tcgtcaagtt ctgtttcatc
     6061 taataggtca atgttcccct gcataacagt taatggagtt ttcaaatcat gagctaaaga
     6121 tgcaatctgt tctctttgtg ctttttcact tcgccactgt ttttctaatg acccttttaa
     6181 actatttcgc atactatcaa aggatataat cacgttttca aattccaata ttttagaatg
     6241 tccgatagta aaatctaagt tgttattctc aatttctttg gtagcttcta aaataggtgt
     6301 caattctttt ctcagctctt tagcaaaatg aacagtcaga taaacacata cccctaagct
     6361 gcttatagtg attaaagaaa tcatcataat ttctggggac ggtaaatact gattaaattg
     6421 tgcatttaaa tattgagaac ctataacata ctgtaaaaca atatattcat catctcgagt
     6481 aatgaaaata aactgtgttt taccacttgt atccatgaac cccgttttag caaacattaa
     6541 agccttttct ttttcttctt cactcatatt cgtatctatg tatccatagt ttttagttaa
     6601 tcgaagaaat ttagttccag taggaaaatg tatatctgat acatttggtg ttgcagttag
     6661 tatcggtact aaagctttcg tttgccgttc gttattatct gcaagtgtta caagccctat
     6721 actggaagca aacatcatac aggcaaaagg gacgacaacg gacaagcata gtccgccaag
     6781 tagacaaaca agaaatttat aaaaaattct tgtaagtgat gttttcattt ttattcccat
     6841 ctataaccta tcccccaaac tgttacgatt ggctgaacac ccgttttctc aaactttgac
     6901 cttatatttt ttatatgtgt cgagattgta gaactatcgc tttctccata aagggaaaat
     6961 gtagcttcgt atatttgttc ctttgaaaaa acctgtcctt tgttactagc aagatactca
     7021 caaatcagat attcactttt cgttaaaggc actggtatat tatctacccg taattctttt
     7081 gcagataaat caatttttat ccggtcaaat gtaagagcta tatgtcgttc tcggtgttcg
     7141 cggcgtaagt gggcatttac tcttgcccgt aattctgtaa tacggaatgg ttttgtcaaa
     7201 tagtcatctg ctcctaatcc aagaccgaat gtaatatcat tttccattgt cttagcagtc
     7261 agaaagagga taggacagtc cactaatgaa cggattttat cacaataaga aaacccgtct
     7321 atatccggca tcataatgtc cagtataatc aagtcatatc tgttcagttt atcaagagga
     7381 acctgtgcgg cggacgtata gactgtaact aaatgtccgt ccttttgcag tccatttttc
     7441 acaagttcta aaataggtcg ttcatcatca atcataagga ttgttgccat agtatcgcct
     7501 cctagctttt ttagtatatc ataatccata aaggcagaaa aacaaaaatc ggtcattgac
     7561 gctttgcaat atagtagcgg tcatttatct gtactgcaac ggcttcggtc ttttctgtac
     7621 catgaatttc ataaatatct ttgtaaatcc attgttcccg atgttgtccg gcgttttccc
     7681 cataccagtc aatttttctt agttcctcct ttgacaaagg aatttttgtt tctgcattaa
     7741 atatgacatt ctccgcaaga ataccaatgt aacttcccaa ttcattattt gatacagtat
     7801 cagaagttac ttgataaacc gtcccatcac aaagaagctg cgttgcattt tgaggattga
     7861 tttcaaactt tccactcata gattgctcta tatccttaaa gtcaaaaact gtatctgtgc
     7921 ctttgatatt ttcatctata acagctttgt gataccctcc atttatatct acaatcagat
     7981 agtcgtcagc gttaggagct gcatacacat ttaagaatgg aataatatag gcgtcattag
     8041 gggctttatc cactaaatct gctaaatctg ccaacgtctg aaaggtagca gttttgagat
     8101 tttcttgaag taatattttt cctgattcgt ctacagccgc aagttgtcgg atatacccaa
     8161 tccattcccc gagtccacta ttagagacag tatccgctaa aatcgtgtag tcatttccct
     8221 tataggaaaa ttgcgtcaca ttttccttat tcagatagca ttgctctctg gaatagtccc
     8281 cgattttgtc ctgtaaagaa cagcctgcaa gagataaaca tataataaca gcaaacagca
     8341 atatcatcat ttttttactc attttctttt actccttccc atctctgaaa ccacactaag
     8401 cttatgataa acagcactac tgtaaaaacc agcatgagta taaaacctag ttgaaattcg
     8461 ggtaagttag aatgaaactg cccctcatta acatatctta aaacacaata atccataaat
     8521 cgtacacccc agccccacgg caggaatttc cagataaaat ctccaagtcc tgtaactagc
     8581 aatgcagata ataaacttcc tacaatacct atgcccatag atacactttt gcctaaactg
     8641 aaaccaacag gtatggaaat aagataaagt ggtattacag acaatatcaa cataataaga
     8701 ggttttatat aaaacgaaaa tggcataact ggatagagta cggcaaacaa gaaaatacaa
     8761 agtattgttg caaaggttgc taaaacgata agatatacta attttccgat atataccgct
     8821 atacgtgaac ggatcgtacc taagattaac tgaaaatgac ctgctccatt ttctagttcg
     8881 actatcatac ctacaacgat accaataaca aatggaaaaa taattgctaa cacctctaaa
     8941 aaggtcgtta catttgtcat ctcactccaa ccggatatat gaaaatatcc tgcaaaaaca
     9001 ccagctccta ttaacggaaa aagcacatgg actaaaagta atggtgtatg tctgctttta
     9061 agccattctg cttgaaaaca tctgataata ctaaacatta aagaacctcc tgctttataa
     9121 aagattttgc cgttgacttt gaaattgtat atagtaaaag caatgacaag cagattgtca
     9181 taataaccaa cggtaaagga gttgataaag cacttcctgt tactgattct ccattcggta
     9241 atattcctaa tgtgggaacc ataaggcgag taacccagct ataaggacat atgaaccaaa
     9301 aacttgtatt tgcccctaat gttcccaaca taaagctcat tccgacattt aatatgagag
     9361 tggcaaatac acccactttt tttgtaaacc acaagcataa tggaatattc catatacttg
     9421 ccagaataat gcaaagtgtt ccgaacaatg cctgcattgg gctaattgtc ataggtatat
     9481 caaaaatgtg ttgaatgata aaaccacctg caacattgca taacatcaaa attgtatttg
     9541 caagtacaat gtatattata caagtgatta tttttgcata ccatactttt tttaagtcaa
     9601 caggcaatga caaaattgct ctaaatttta atttgccgtt atctctttga attatcaaaa
     9661 tagataatag cgttaaacaa ccaggatata agaatacata ccaccagtta tacgaatttt
     9721 gttgatacca cataggagca agtatattca gacataaagt tagtatagga gctaaccata
     9781 ctaatttttt tgcgaatgta tttttatgtt ttaaatattc agaagatata taatttttca
     9841 ttttatcgtt cctcctgtga tttccttaca acttctacaa acagattttc taaattttca
     9901 tctttattca tctgtccttc atatcctaaa ataccatttg aaatgatacc aatatcatca
     9961 gcgataagct gaacctctga tagaatatga ctggatagaa taacagtaat ccctttagag
    10021 ggaaaagaac gaataagaga acgtaattct tgtatgccga tagggtctaa tccatttgcc
    10081 ggctcatcta aaatcaacaa tttgggacta ttcaacaaag cgatagctat tcccaaacgt
    10141 tgtttcattc ccattgaaaa ttgtccagct tttttccttc ctgtattttg caaatctacc
    10201 atttttaaaa ctttatctat tcgggtttta ggaatattta gtaattttgc acggacttct
    10261 aaattttcta ctgcggaaag attttcataa agtggaggag tttcaatcaa agctcctatc
    10321 tgttctaaat cttttctgtt ccattcatgt ccattaaaaa gaactttacc gctagtagga
    10381 cggagcatac ccgtaatcat tttcaaagtg gtagattttc cagcaccatt tggaccgagt
    10441 agcccatata ttgaattttc acggacagtt atagatacat tattcactgc cttttgcttt
    10501 ttgaaatctt tacataggtt atttgttttt aaaattgctt tcataattgt tcataccctt
    10561 tccttatttt ttagttaaag tataaaaagc aaaattaaag attttatgaa gatttcaaaa
    10621 tattatattt taatctagcc gtagcgtgta acttgcaata aatgcaaaat aaagctacgg
    10681 cattttttga tttatcctac aatcttccag ttactatcct tatgtaacac aagttcatac
    10741 tgcgatacct gcattgttaa aaaaagtttt tatctgataa tattttcatt aagtaaagga
    10801 gatgtcacaa aatgaaatta tttatcggtg atattattca gaaactaaga aaaataaaag
    10861 gtctaacaca agaacaagtt gcaaatgctc ttggaataac tacttcttcg gtgtcaaaat
    10921 gggaaacaaa caatacatat cctgatattt cattactaag ccctttggca agacttttag
    10981 gaacagctgt agataatgtg tttaatttca aacaaaaatt atcagaagaa gaaattgaca
    11041 ctcttttgaa tagaagcaaa taacgctttg gtatgtcata ttgatctttc attagtaaaa
    11101 atatatgaat tattgaggat ctttttacga tttttttagt atatttatat tgtaaaaggc
    11161 aggcacttac taatattttc atgtttcttc ttttctttat ataaatgttt ttccattata
    11221 ttccaaaact tatttgctga tccgctccgc aggtaacaaa taagagccaa caatctataa
    11281 gttacaataa cctataaatt atcggctctg cgtcttagcg tctggctctt taatagtcgt
    11341 tacatttcca tgctttatat tgattaaagt ttcatgcttg cactttgggc agaacaaagg
    11401 gaaacttatc agttccgtat ctacccgtat ttttgttcgt gtcttgttat tgcagatggg
    11461 cataataccc atgattgttt cattacagaa acaccacctt tattttaaaa gttcatcata
    11521 gtattttgcg tttcctgtcg agtaataatt gtaaaaagca gtaattttat tgtcagaata
    11581 cacattaagg taatttagaa tatttttcgt ccattctaat gaacctgctg aatttgctgt
    11641 tactaaattt ctgtctaaaa cggaattaga acaaacgtac aagctctgtc ctgcatagtt
    11701 tcttgaaaag aaatttagat attctagaga attacttgtg tgtttaaatt tatctaatat
    11761 accataatca gcaagtgcga gtgttgcccc acaaatagca cctactaaaa tatttttgtt
    11821 taaacattct tgtgcttttt ccaatatttc ttgattttct ttacttcccc aagtggaaga
    11881 acccggtaaa agcagagctg tactagcatt gaagtcaact tctgatatac tataatctgg
    11941 aacaattgtt aatcctccta aagttaagac gggttcttta ttaaaaccaa ctgtctttac
    12001 ttcaaattct ttagttccat tttttaataa tttttccata ctgaatgctt gtaaaatata
    12061 accaatttcg cattctgcca tgtcgtctaa taaatataca tatacttttt tcattttcac
    12121 atctcctcat attttcgata taagaaaatt atatagcagg aatagtgaca aatgtatgtc
    12181 actattaagt gaaatttttc tttatttttc tgatatgagt taataccttt tctttgtatt
    12241 tttgagggga taatattttt actttatcgc aaagtcctaa aaggattgag aatgtcataa
    12301 aatcattttc tttaatctca atttctctta taaaacagtc attgacagtt tcaacaattt
    12361 ttcctttaaa gtattcatta actaaggtgt ctatttcttg tttataagat agagtaaccg
    12421 taatatttgt ctggttgcgt cttttttcaa aatcagataa aaggtcagct atattatctt
    12481 tggtttgaaa aagttctttt gttaaagaaa tatttttcat tcttaccact ttgaacatgc
    12541 gataatcatt tttagaggtg tcatatccaa aaacatacca ggagtaccac ctataatatg
    12601 tatgtaatgg gtttagaaaa actttttttg tatgtcctgc tgaattagta tatataaatt
    12661 ttatagttcg ctgttctttt gttgctttat tgattaagtc tacgcacctt ataatttgtg
    12721 gattttcagt tacaacagag aaatcaacat ctggaagtgc attaatattg acatcgttat
    12781 aaagatgttt cattttttcg taagtttcaa tgatcttttt atttccgtaa acagttctta
    12841 gactatttaa agcaagcaaa atatattctt tattgttgct gtcaataatt ttttcatcaa
    12901 ccttaaaatc tttatttatt atatagccac catttacacc taactcagaa taaatcggta
    12961 taccagcaag agttagtgta tcaatatctc gtaaaatcgt tctgcgagat acttcaaact
    13021 tttgtgctaa ttcatttgtt gttacacact ctctatttat taaataaaag attattccta
    13081 tcagacgttc tattttcata ctttcagccc tttaactgta attatataaa tatattcttc
    13141 tggtgtaggg cgtttatttc caaaatattt tttgaaattt gcctgcacct ctgggtcggt
    13201 gctgttcata gcttcatcaa ttgtcgcttc aatatccgcc cgaacatcag ccaatgacat
    13261 accgccagct tttgcaccag ctttcaaggc ttttttaata tctcgttttt ttagtcctat
    13321 catagagttc actcctaata atttattttt ccagtaatat taaacacccc aaaggtaaat
    13381 acataaacag tagtgataat cgttgccata agccctcaaa agctatgatt gtatctttgt
    13441 agtttggttt atctgccata acaaaaatcg taaaaaagat aaatgctaga agaaaacaaa
    13501 tcattgaaaa tagtgcaagt ggtatttcct caatccgaaa ccaatataag ctaatcaaaa
    13561 gcggagcaaa agttaaacat ataaatccaa gaaccgcccc gtaaccatgt attttttcag
    13621 ggatagtatt gagagatttt gtttctccta ccgaaaaaat ccctgagaga atacagccac
    13681 ctatcgcata tatcaatatg actataaaca aaataacagc tatgtttttt gatttctgcg
    13741 aaacaatttc ataaacctta aaaccgctca ttaaaagaat gataccgcat aaaactagcc
    13801 ataagttgta tataaaatgt agtggagatt tgttatttcc aagcacgctc attacctgca
    13861 tagaatgact ataaccttta taaaacggag cgaggaaaaa agggatagtt aaatccagca
    13921 ataaggctaa tggtaagaat agagaaatgc tatttttcat cattaaaccc accactcaaa
    13981 ctaccaagcc cttttaaaat ttttactgcc agttctttca tcacatccgc cttgacaatc
    14041 gctattcctt gttcttcgtc gccaagaact aaaagtgtat cgccagcgtt aatatcaaaa
    14101 atttcacggg cttcttttgg gataactatt tgtccccgtt ctccaacttt taccgttcca
    14161 aaaatatgtt gtcctttttt catcattttt ctccttctta tcatatttat ttacgaatca
    14221 tatttttcat atttacataa atagtataat gcgaaaaata agaattgtca attccttata
    14281 acatgggtag cgtgcaaatc tcttatagaa atgcacgcca tatttgctta tcctacaatc
    14341 ttccagttat tatacttatg tagcacaagt tcatactgcg atacctgcgt cgcttttgtc
    14401 tgattatcaa ggaacttcac ggcaaccttg acttttacat tgtccccgtc ctttgtaaag
    14461 atagggttta ccaattcaga ataaaagtag tccctgccga taggttcaag cacatttccc
    14521 gatacatagt aagcaagttc tttttctgtg gctgtcgggt agagcttaaa gaatgtttcc
    14581 aaaaatgagg tagcgtcatt tacggtatca gcgtccacgc ttgcgtctgc ttctggtgtt
    14641 ttaggctcat agtctgattt ttcgattgct ggtgcaaggg tagggttctg aacgattacc
    14701 atatccccgt cagcgtccac atggactttc acggtatagg ttgctttaat tgctgttgtc
    14761 tgttctccct cttttatctg ctgatctact tcgtaggtga cagaaaaagt gtccgttccc
    14821 gactgttcga tattccacac aatcacatct gtaaccgtgg aactggtcgg tatatcggtt
    14881 ctaatggtat ccacattcaa gtcctgcaat tcctttgtca gataaccgtt gattgcctgc
    14941 gttcttgctt caatcgcttc tttgctgtta ttccatgtgt aataggactt cgcaaagttc
    15001 ttcacgaaat tttctatccc gttggtgtcc tgcaagcgaa gttcaatgat ttctttttca
    15061 tgggtcgtgt gctggtcgat agccgtaaaa ttcttataca ccccgaagct cacgcttgcg
    15121 ataagcacca cccacaacgc aattacggtt ttctgatgtg tgcctacctt gacagtacgc
    15181 accttttttt ctttgacatt ttctgtctgt ttcttattct tcttaaacat atttttcaag
    15241 tcctttctat tgttttactc gtcctgcacc gattaagtgc tgttgccagt aactattatt
    15301 taggtctgca tatcctatcg ggtcgcctgc atgatatatc ttgttattcc ctacataaat
    15361 tgcaacgtgc gttacataag taccagcgtt ataggtagaa tggaaaaata ccaaatcgcc
    15421 agcctttgcc tgcgagagtg gcagatgttg ggttgcgtca tactgggctt gtgccgttct
    15481 cggtaaggag ataccagctt ttccatagca ccattgcgta agtcccgaac agtcaaagga
    15541 agtgttggga ttgctaccgc catacacata cttccagcct tgatatttca acgcttcatt
    15601 catgaccttt tgtgccagct ctcccgatac ctgcggaaca gttaaatact gattgactaa
    15661 ttccacataa aattgattgc catactgata acgccagcca ccatttttcg caacggctat
    15721 cgggttggtg taagttactt tctttccacc cgatttctca cgggcgaagc tctctgcaag
    15781 attgtatgta tgtttctttc cgtttcctgc cacatatccc acatagccac caccatagtt
    15841 ataggactgt atcgctacat tcaagtcgtc cataccttga tttttgcagg aagaaagcag
    15901 ggacgcaaaa tacttacacc cctgcttgat tgagctttcc gtatctaaag agttgggcgg
    15961 taaaccaaga ctttccgaac tctgcataac atcttctgcc gtaccgccac tttctacttg
    16021 aatgatagcc agcaacacat tgacatactc ggagataccg ttttctctgg cgtatttttc
    16081 taccataggc tgatgtttta agacttctgc ggataggttc atacccgtaa tgctagaaga
    16141 aaagttgctg ttctcgtcgt cgctgtccgc actaatcaag acaccaaaga aaagcaccag
    16201 agaaaagaga ataggaaaca gacttccaat aagagcgata tgtttcaatt tcattttttc
    16261 ttctgtcctt tctttgttac aaggttacgg cttttctctg tggtctgctg gttcttctgg
    16321 acgctctgcg tctgctgaac ctttattctg cggtctttgg tgtcattctg gcgtgtttcc
    16381 tgcgatacca ctttttctac attctgccta tgtactggct gtgccgactg ggtcgctttt
    16441 ctatcagaag cacccgaaga tagcggacgc tctttgataa catttgtctt gactggctca
    16501 cttgcttttg aagtggtcgc tgtggcaggg cgtttgactt cctgcatttt ttcagcactc
    16561 ggttttggaa tggttgaagc tgtggctggt ctctcatggg gacgggtagc tcccgttgtc
    16621 gctgatccgt cagcctttcg ctgtgcctgc cttgcttctt gtgccttttg aagctccata
    16681 cgcttgtcag caatattttt cttacgctgt tcctgctttt ccaaacgtcc cgtctgtctg
    16741 gactgctgtt cctgcaccat gccacgcttg aagtctgata cgctggactt tgccttttcc
    16801 tttgcagaat ataccgcata agctgtttgt gtcggcatat ccttgatatt ttctttgaca
    16861 gcgtttgcct tgtctttcac tttatttttc gtgtctaaga cagcacccac ctttgagcct
    16921 gcacgctgtc ccatgctgga agtggtatta ccccgatttt ctttagaagc cgtatttttt
    16981 ctttcggctc ttttgccagc aacggcactt ccagccaccg caccagcaac accgccagaa
    17041 ataccgccag cacttacagc ccttgcaata cggtgttcca tacgtctagc ccgatgtcgc
    17101 ataaacagat atggtctgcg gaatatcctg cgtcccatgc tttgactgtc gccagcatta
    17161 agagagaaca tactcattaa atcgcccagc ttcatataga taccagcgaa acacactatc
    17221 tgcaagaacg ctaccatgaa aaacgggtaa tctgtggaaa tgttataaaa catactggaa
    17281 atgctgaacg ccaccgttac aatgagcgtt attcctgccc gtgtcattat ggtattaaat
    17341 accctcacga ttgcctgctt tgccatgctt tcatagctcg gtatcatgga aagtagaaag
    17401 ctgataggca aaaacattgc aaagataata aaaagtatct ggctgaacaa catcataccc
    17461 gtaagcaaga atacaaatat cgtgatacct aagttgaaga acaagaggaa aaacaccata
    17521 cccaaacggt tcactacctg cggtattgtc agattgttgt tgtcgttgtc ctcaatttct
    17581 gttttcacga cttcctctct ggttttccca tcctcgtcct ctggacttgc cgataccaga
    17641 gcttccacac ggtctgtacc gatttcttct gcgttgctgt tcccgaattg taagagtagc
    17701 cacggctgtt ctacttgaat agaaaataag ctgtctcgta tcaagtccac gctgtcttta
    17761 ctctcactgt cagagttggg gagcatgatt tttgttccca agtccaaaga agcggtgctg
    17821 atgtctgatg aaaattcatt tatcttcttg atgtagtcgg gagcgtaggc aataaatgaa
    17881 gcggacagaa tgaacaccac cacaaagttg ataacagcgt gaagtgcctt gctggtttct
    17941 ctttttatca gtcccgtata agcaacataa attcccacca ctaagataat gagaagcagg
    18001 aaaccaatat agaagcccgt agaagaaaag ccgttctcgg taacgcctga aagggtctgt
    18061 atgctctgtc cgatactgtc tgccatatcg ttgatgaagt ccagtttata ggcttcctgc
    18121 accacatagc ccgttgcatt gctcaaataa aggcttatcg tccagacaaa gttggtaata
    18181 cagtaaagcc cgtactgcac cgattttcca ataccgtcca gccagttcca cggtagccag
    18241 ccccagctat tatccacata gaaatcaagc tggtaattgg aaagcggata ttttgagtac
    18301 agattttctg tattgacagt gtcgtccaca agccccgtcg catgagccac cgtcccaagc
    18361 agagagagaa acactatgct gataaagatt gccagcacag ccactttgac aaaatgaaag
    18421 agcttccgtt ttgtcagaag cccctttagc ctttctttca tcactccacc tcatttcttt
    18481 gtacgggcgg tctggtatca aaagcgtgta gcagttcttc aaagactggg tgtatctgca
    18541 ccacaccgac acgcccgtat aagtcctgca ataagcattg tccgttctct aaatcacgca
    18601 agcgtttctg gttgttctcg tcgtccttgt cgataccgaa aaactctaag gtctgtttta
    18661 tctcgttgat gtcagtagaa cggaaagcga atttcaagcc gatattgttt ttcagacttt
    18721 cctttgatac gtcgccagag gattgtgtaa cgaaataaac acctgcctgc atagctcgtc
    18781 cagcacgaac cagcttattt gaaagtgttt ctccttgtgc cacatttaag aacgcccacg
    18841 cttcgtccaa atcgacaatc ttaaaaatgc ttctgtccga atggataaaa tcaagggcaa
    18901 aggtactaat cacaatcagc atagacaccg acaataattc aatggtcgtg tattcctcaa
    18961 aggtggtatc tttatttggc aatactaagt ccgctacttg aatgatattg agctggttat
    19021 caaggctgat agcattttcc accgtaccgt ctgaaaacag caggtgtgca aagtcgtagt
    19081 ccgtgaagct gtcgatatgg tctgcgatat tgcgtgagat agcagtatct tcctttcgca
    19141 attcctcaat aacgtgtaac agtcctctat tttcactctg ggtaacggaa cgaaccgcct
    19201 tacgaagtac ggggaatttt tcgccgtccc tagaggaaat acccgtaagg aatgtcaaga
    19261 tgtcgattgc cagactttca gcgtctttta cattcttcat aatcacaaac gggtcaagaa
    19321 gtcctgcatt atctttatcg cttgttaggt ttacgatatt gatttcatgg gcgatttctg
    19381 gaagtgtttc tttccagttt ccacgctcac ttttagggtc taacagaagt gcttgaccgc
    19441 caaacagcac cgcataatat acaagcagat tgttacaaaa cgacttccca ccaccaagcg
    19501 aaccgacaaa agcagaagca agggcatttg taactgttcc ttttacgccc tgcgaagcta
    19561 aagacggttg caggtacaca tttcttcccg tatcaacgga atagcccata tagatacccg
    19621 tagtttctcc taactgctgt gtagcaccaa aaccaagccc tgctagaaaa tctgatttta
    19681 catactgcac ataatcattg atatatcgct tgctggcagg taaaaattct gaatgaagcc
    19741 ccagcatatc cccagcagga cgcaccagct ttacattgag gtcgtcgtaa aagtccttga
    19801 cttcatcaca acggcgtttc agctcgtcaa gatcgggtgc agacacccgt atcacgtagc
    19861 ttaacttata catactttct ttgctctggt ctaagtctgt ttccagctcg tccacgctgt
    19921 ctaaagcgtc caccacgttt gaaatggttt catttcctgc ttgataggcg tgattatcca
    19981 aatctttcaa ttcttttttc ttgtttcgta ctgtggtaag agcctttctg ttctccacga
    20041 tttctacatt catagaagta tcaacgggga atatgaattg ttgttgctgg aaatagaaga
    20101 tttcacttga cggaaaatca agctccccga caatcgcatt gacggtaaaa taggacacat
    20161 agctttcctt gtcctcatgt tccaagcgta aataccgctg gctttcctca atcacacacc
    20221 ttgtcggacg gataaggtcg tagtatttta tcagcgtttc ttttttgtag ttcttctttg
    20281 gtagctggta ctcataatct tcataagcga caccgtccct gccgtaaaga tgttccatga
    20341 gatacccaaa atcatggatt tccaagcgac gcaccttaaa gcgacgggag attttatttt
    20401 ccaatagctt ttccattttc atataacggt tgatttcatc attcggcatg gaaacaaagt
    20461 cattcatcag cgtgtggttc acttcatgga gaaattcctt gaacgtcagc catgccgatt
    20521 ttttgatgtt cttcagattg agctgttctt ccgtaaccat gagcttaaag ccaagaaaaa
    20581 agcggtagtc cacttgattc tccccaatca tagatactaa cgcttcggtc tgctcgtcta
    20641 tcttctggta agcaacttcc tttaattttc ccgttaccag cttctttgac tgctcctgca
    20701 tacttctaat tgatctttct gtggcaatct gcaacgcatg aatttttccc tcacgggact
    20761 gtgcgattag ctgtcgaaaa ctgtcatgca cgataaattt ctgctctgcg gatagaaaag
    20821 aatagttgta cggtatcagc tcatagtagg cgaacacctc attgtccttg ttccagacaa
    20881 ggttgttgtt aatatatttt atcgggaaca tagttcacac tccttactgc cgtgattgtt
    20941 tcattcaata tctgcttatg cagttttacg gcttttcctg cataagtgat tttaggtcgc
    21001 agggcatagg ttatctgtga tttcaaaaag ctgtacggct tctttccgtc aaaggttttc
    21061 tgcgacataa accaagtgag agcaacggga ataccgaagt attttaaaaa tgctccctca
    21121 atcatggaaa gtgggggcat atccccaaac agaatgatga gaaattccgt aatcacaaac
    21181 catgtaatct gtgtaaaggt aacgggaaag ggtaagttaa agtcattgat tgcatataag
    21241 actttttcca cgttccagat acccgtgtag cttttaatct ttttcaagtt ctttcagccc
    21301 ctttcgtaag ttcttcaaag agaaaaggac agccattttc agaccgtccc ttaaagagaa
    21361 atacgctgtc agtagcaaca atgcttgttg ctgatcttcc agcgttaata tggtatctcg
    21421 cacatacctc ggcttgtttc aatgaagcaa cctcccatat ccaaatctcg cccgtaggct
    21481 tcatagtcaa tgtagttctg caagctgggt ggaatgtcgc ctaatgcctg caattcgtct
    21541 atgtagtagt aggcaacatc tgtcatggtt tcacagtctg gataataata aatatcgtcc
    21601 ttatgttcca cgacttcttc cagcgtcccg tagtggctga taaattcgtc cagacactct
    21661 acgatatagt cgggaagttc ctctatcatt tcatacatag agttcagttc ttcaatggaa
    21721 acatactcgc caatcgaaat agggaagtta tcggtatcat ggattgcgta ttcctcatac
    21781 tgttcattca agccgatttt ttctttcaca tcttcctcgt caatgggaaa tgtaaaccaa
    21841 gcacccacta aatagccctc gttgtatttg ccaagattag caatataaac tgccatatca
    21901 tcaatcataa gcaccacctc atttcctact ctggcagttc aaagacacta tgttccgtta
    21961 caagatagcg tctattttcc agcaagtgag aagcgtaggc ttcaaagtca aaatagtatg
    22021 cttgacagtc ctcggacaga tgtttgaatg tcgggtcatt catcagcttt tgttttgcaa
    22081 catctatcat gcttttacag tctggataaa ctgtaatcac gttcctgcaa atataaagtg
    22141 cttctaagtt ttcaaacacg gtaagaagtg ctatatattc ttcctgcata tcggctggta
    22201 gctggcggta cataaaatct aattcattga gctggtacac gcttgtttgt tcgtggactt
    22261 catcagcata aggcaacacc ttttcgataa tgcggtaatc ttgactttct gcaccgacac
    22321 ctaacttttc ttcaaactcg gcaacatcta tcggcaggtc gaaccagtat gatgttgttt
    22381 ctttgccagt tgttcctctc gtttccacca gcacccttgt ttcctgcatt gttcctcacg
    22441 tcctttctgt tgtttcatca catcttttaa gctctggtac gacataccaa acacatactt
    22501 tgaaaaatct tctaactgtt ttatatcata attcgtaagc ggtaatcgct tcatttcatg
    22561 ccacacttta cgcttgtaag agggcaggtt agaaatataa tcacagccga cgtttgcctg
    22621 catatcctta aaaatatcgc tcatttcatc actccaatct gattttaggt aagaaaaaag
    22681 cagtcaatcc taagactaac tgctttgtgt atatggatat aaaatctaaa ttctattgta
    22741 taacttttct tagccaatta tctctaaaga aaatctgttc gttagtatga aaccaatgtt
    22801 ctccgtcatt cataacggta agatttgcac aatgtctatc aataaattta tctaccgttt
    22861 gacgtgatgt aagattatca tgttcagcat acaaaatttc ggtaggcgtt ttccaattta
    22921 tcgggttttc ccgaacaaaa cataaatatt cccacgataa tgtttcgcca aaatcagttg
    22981 gtatttcttt tctttcagaa agttcctttt cagacacatt cgcccagctc atcatatcta
    23041 ctataagttg ttccatatca agtataggcg atatgaataa tgctttttca atcttgaaat
    23101 tctgtaatgt gtgcattgta aaataagctc cgatactatt agcaagtaca tatatatagt
    23161 catatttttc agatatctta tcgtagactt ccataatttc attttttaca atccacggga
    23221 aatagtcatt ataatctact ccaaaaacat caaaatctaa acaaatattt ttgaactgtt
    23281 cggcttcaag ataacttcca cctttaccat gaacatatat cattgctttc ttcaaaagaa
    23341 tacccccata aatcaaaatt ttatatatcc atattataac attcgcatat tagcaccaca
    23401 ataaaaattt atgcaccgat aatcttgttg aacagttcta acagtacatc tttcacaccg
    23461 ccagcattga acaccaaacc gacagcaatc aaggcaacta ccaagaagcc gatgagcttt
    23521 gaaaactcac gcttgaaccc caagtagata ccgataacca caatcgacat aagcactaag
    23581 ctctgtgcgt tgcttaaaaa ccattgatac aaattttgcc cgaaattcat gttatttgtc
    23641 ctcgctttct tttaatttct tcatttcata gtcagcttct ttgccaacac ttaaaataca
    23701 catcaaggct acgccgatac ccgttccaac agataccagc aggaagtctt tcaataattc
    23761 ccacattttt tcatcactcc taactttcta ctaaatcttt tgcaggggtc gtctgctgtt
    23821 tgattatcat ttcatgcttt tctgtgagct ttgcctgctg ttcgatagtt ttcatgtagt
    23881 ctgttccgtt tcccttatca atccttttca gcattttcaa ggtcggtgct acctgccttt
    23941 gtacccaacg aagcgtgcgg tctaaggtat aaggctctgg ctttgtcgtc agtttcagac
    24001 tttgccgatt atccccgata aaccaagccc agcgctcgtt gagtttccag tcattttttc
    24061 gcttgtcggg ttcttcatca acaaaccgca catactggtt gatgatagaa aaggcggtct
    24121 gctctgcgtc ataataggtc agcaaatccc gtactgcata gtaagcacgt tcattgcgaa
    24181 gccgtatctc aaaacggttg ataatttcgg ctttttccaa aggtgtccct aacttgacat
    24241 actgctcata gtctttttca tagatacaga aatacacatc tgatttcagc gaaccaagat
    24301 aaagggtgcg tcccatatat tctctgtcgt cctctctgtg cttgataagc tcgcccgact
    24361 gataaaactt atagcttctg aactttccga tatattcccg tttcctgcat ttttccgcaa
    24421 gctctggaat atccaaaatg cccgtatggt cgttgatagc aaggtcaata cgcttcatca
    24481 cgccaccgtt tacgagtgcg tccataagaa agtcatacca gcttctttgc tgtgccagca
    24541 ggtaactttc aaactgcctg caaccacgcc cctttaactc taaaaggaca cctttttctt
    24601 cgtcagccga cgtatagata aagatgtccc ctaaagaata atgctccgta taactgtaat
    24661 gtccgtaatc ttcatggagc atataattga tattcagttt taatatatct ttgatgatgt
    24721 gctgtatatc cagcgtggga aaacgaattt ttacataatc aaacagcatg gtaagcggtg
    24781 cttctggatt gaaccttgcc agttctttat gcagagtttc cagaagttcc tttgacggct
    24841 tcatttttcc gctttctatc ttgttgagat attcccttgt gatatcagaa gccaccgcca
    24901 gtctgccttg tgagataccg taagcaatcc gtttttcccg taattctttt atccattgtt
    24961 cttcattcag aaccataccc ccaatctgtc aagtgtgaag ttttttaagg cgacttcaca
    25021 gccgattttt gctaggaaac catgcgataa gccttatgtt tcctgtgttt ttcgtctatt
    25081 gtctatttgc aaacgtaccc ctctgttaga taccgagggg ttttacctgc tggcgtggct
    25141 tcgccacacc agcaacggct agtccgtgcc gtcgcctttc gcttcgcacg tcgccgtccc
    25201 gtcctgcctt gcttgagcaa gagagccgat agtctgcaaa aaatcatgtc ctttcgggac
    25261 taaaggcgtg taaaattcgc ttatcacgct tgttcccaca tcacaatagc cacgcccctt
    25321 gatacgtttc tggaaaaact gctttttcac atctgaacca aaaagcatac cgtaacctaa
    25381 ttcgctgata cgcccaagtc ccacacggaa attgaagtta tctctgatac cgtccgagaa
    25441 atactttgcg tctggacgct ggcaggcaac gataaggaaa taacctgctt gtcgtcctaa
    25501 cataacgatt ttctttaact ggctaagtaa gcccacgctt tctttcgtcc ccagcatttc
    25561 aaaaaatgcc acatattcat caaagataag aaagcagggt ggcagtccca gataggcata
    25621 gttttcgccc gtcttatagt tcgggtgtcg cttcatttcc tcatttcgct gtaccatgcc
    25681 ctcataaaag gcattgacgc aatcaatcat ttcttctttg gtgtgataca cattttccat
    25741 aactgtccct aagtctgcaa ggtcagcgtt cttcgggtct aagatataaa ggatagcgtt
    25801 ggtatgcagt aaggcttcaa tgagtgtcag cagaaaatat gttttaccgc caccagtccc
    25861 accagcaatc aaagcgtgag ggagtgcgtc atattcccag acaagatttt tcatcagtct
    25921 aagacagccg ttctctgccc gtacttcatc aatggtaatg cggttcgcta tcatatcata
    25981 aagcagggta tattcgatat agccgtcatg cagggtcttg tcggtcagct cacaatacaa
    26041 gccactttcc aatttatcct ctaaccgtaa aagctggtct tgatatttcc ccagcgtaat
    26101 ttcacagcgg atatgaagca gtcccttttc catttgataa taaatctttg gaaaccagac
    26161 tattttttcc cttgatctgc tttgcaggtc agtaaaaaaa acgctgtctt gtacggtatc
    26221 agcttcatac cacttgtttt ccagtatcat tcgtgccagc ttttgacggt gcagtagctt
    26281 tttgaaactg tcgtaacaga agcggtaata cagaaaagcg accaatgcac aaacaccagt
    26341 cgctatcagt atggttatga agttgtaaag agaaagcgta aagccattgt ctaataagct
    26401 gaaatgttcc cagtcggtac gcatgagctg ttttgtattt aatagcagga taaccgctac
    26461 gaacacaaac aggagtgtcc ccattgaaaa gcgatagaca aggttcttat cgctggctct
    26521 gatacggtgt cctttgttta aaatcattct cataaatcaa tcactccttt ctgggtatga
    26581 aaaaagcagt caatcgtata gactaactgc tttgtgtgca tggatatgaa gttgtcaaac
    26641 tggaatttaa cgttcactca tatattgttt tatttcatca gaagtcaatt ttcgtacttc
    26701 tgtatgtgaa tattctttat actctccaac attaaacaca ggtgtcattt gtttgcattt
    26761 cttgtcttta ttctttttca agaatagatg taaatcctca ttcccggcga atattttata
    26821 agatactctg cctttactgt tctctatctc ataaatacca catgaacttt tcgttgtata
    26881 atcggctgtt cgtaaataaa gttttgcaat ttccaatgac ttaaaaggaa aattccatct
    26941 ttgtaatcct ctgtttgggt catgttcaat acaaatgcaa cgtccacaag ttccacaaat
    27001 atattgagtg tggttttcta agcagtccaa gggatttagt aatggtgtta ttctattttc
    27061 atcagagtaa cattcttcac acatttttct tttcgctcct tatcaaattc taatttgtcg
    27121 ctttcttcat acccatatta taacagttcc gtatatcctc tacaacgaaa atctattcag
    27181 caagtctatt ttttcggttg tccctgcggt gtatggttct gtgaagtgcc tgtgttctga
    27241 ttgcctttat tcttcagtac aatattctct gcctttacat accagtccac atctgcaccc
    27301 gtataggttt ttcgtgatac cgtatcggct acgggattga caagttccac aactgcattg
    27361 tacggaaatt cccttaacgg tacttctggc ggtacagaca cggggataat tccaccatgc
    27421 agactgcact tcaaatcata gatacgcttt ttaagctggg tactcggtgt cccgtcctca
    27481 ttctgcaaga acacatcacg caccgctgta aattttaatt ctccgaatgt tctctctttg
    27541 tcaataacaa acccgtttga taatctcata aattcttcac tcctttactt ttcttcaact
    27601 gctacaatat catcagctac taatacataa tcggtatgtc ccacatcccc gattgcgtac
    27661 cctctgccgt ataacttcgg attgacaagt tttactttct gttcatactt gaaatgcttt
    27721 tcgccagcct gcacgggaat ttccaccaca acattttcgc ctttctgtac gtcagaataa
    27781 aggttatagc ttcgtctggc tatgaccttg cggttgtttt tatccctttc aaagataggc
    27841 tcgctttcgc ctgcaaattc aagagttcca aaagactgtg ccatatctgg cacgacatat
    27901 ttcatttcca tattgtttta cctcgtttct ttctattttt gataagttaa ttgattgtga
    27961 tttcttattc tggcggttgg ggaagtacca gcaatccccc agatagtaaa gggtaaagtc
    28021 aatgcttacg cacccttgac tatccgtgtt cttgcaggtt gtaggcagac aagccagaat
    28081 aagcggaagt ctgccgtttg acagcttcgg cggagagcgt gatttttctt tcatcatacc
    28141 gttaccgcct tatgatttct tcattttacg gcgtttgtaa agatatgttc cagctaatcc
    28201 accagcagac acaagcaaca tcacgacaaa tgccattaca tttgtactgt cgcccgtttt
    28261 gggactgtcg ctaggtctgt taggcttttc tggtgtcggt ggtgtttcgg gtttctctgg
    28321 ttcttctggc ttttccttaa aggtaatcgt ctgtccctta tcctcaatat ccttatgctc
    28381 ggtaactttc ttcggttcgt ctggattgct aaaatcgtac aattcttcaa aagttacaag
    28441 ctgtttgccg tcaagagaag tagcgtcaaa cgtaaaagca acttccactt tcatagtttc
    28501 gctgtcagca gtaaaggtgt aatcactttc cacacgcttt ccattgataa gaagttctgc
    28561 attttcttct ttcaacatct gccagcccac aagtttgtac tgtgtaccga cttctaagct
    28621 ctctaaggtt acggtatcaa tgattgttac cttttttcct gcttcaagct ctttgttgcc
    28681 gtccttatcg gtagcggtag tatgtatctt gatgatacgc tctgtgataa gtaccgtctg
    28741 cccgtcgtcc tcaatgtctt tgtgttctgc aacttttacg ggttcgtctg gattgctgaa
    28801 atcatacaat tcttcaaagg taacaaggtt cttgccacct aaagcagacg cattgaatgt
    28861 ataggaaatt tccactttca tttcttcatc atcagcgaca aaggtataat cgttttctac
    28921 acgtttcccg tcaatgataa gctcggcgtt ttcttccttt aacatctgcc agccttttaa
    28981 ctgatacttt gtgccttttg taagtccgtc taatttgaca gtatcaacga ttgttacctc
    29041 ttttcctgca aggatagtct tttcgccgtc cttgctggtc gctgtggtat ggatagagat
    29101 ttctttttcg tattcatcag tcaaagtccc taaatcaacc acaaggttac ttcttgatac
    29161 cacgatttca aaaggtggga taagctcaaa gcctttgttg ctttcgcaac gcatttcttc
    29221 aatgatgtag gtatcataag gtaatgcacc cttgctgtcg tctggttcag aagtcccaaa
    29281 ccacacaccg tcctcgctgg tctttcctgc gtttgtattg tgcttatgag aagcccattc
    29341 agcagaagtg gagaattgcc cgttatcatc agttaccact acatgatttt cacccgtcgt
    29401 cttgcttgtg atcctaaagg gaacattagc aagacgcttg tgtgtacctg caccgatttt
    29461 tacaccctca atatctccac gcttaatctg attatagata gaatgagctt cgtcggttaa
    29521 gtccacgatt tttccattct ctgtgattgc aaagtcaatc ggcttcgcac cgtctgtcaa
    29581 gtaaccgtcg ggagcttcac tctcaacgat acggaatttc ccataaggta aaaggtcagc
    29641 agaagtagaa gccacaccct caatatctgt acgaattgtc tttaccactt catttttctt
    29701 atatagcttg ccctcaacca aaacagaatt gtcatttaag gaaatgacgt caaaagcagt
    29761 atctttcaaa gtggcacttc cttgtggttt ggtatcgctc gtttctaaat ctcttttctg
    29821 aattttcaca ccgccacgga taaccttgtc tgatacggaa aactggttac ttcccgtcaa
    29881 tacagcaagg tcgccgtcct ctgtaatttg tgtaaggtat aagcctttta tctgttcaga
    29941 cttatcgcca gcctgcatat atgcaccgtc cagcaagtag ccgtttggag cttttgtttc
    30001 ctcaacagat agtgttccta aaggaagaac cgctttgccg tcctgcatat agaagctgtc
    30061 gccagatacc ttgtaagcgt ccgctaattt tgtgatgtag tgagttgtcc cgtcgctgtc
    30121 tgtttcagcg attgtctttg taacccatgt acgagtagct tcggcaggga gattgtcttt
    30181 gttatagaag ccagcataat acttccatgt aaattctgca cctgctagag aagcgttccc
    30241 ctgcggattg cctttctgtg tttccatatc aatcttgaaa agctcaatca aagtgtccgt
    30301 tactttcggt gtatctgata ctttcaaggt tgctgtcttt ccagcttcaa cctttaagga
    30361 atatacagtt ttatccactt tatatcctgc tggtgcggat aattccttga tatagactgt
    30421 gcctgctttt acctctacaa tatctgtatt tccgttttca tcagtcgtaa gggtggcaag
    30481 ctgtttcgtg cagtccttat cagaaaagac accataggtc gcacctgcaa tagagtaatt
    30541 cccgttaccg tctgtaatgc tggtattact ggaaaccttt tgcagtttcg tatttccgac
    30601 attcagtttc gcccagaatt gtcctaattc ctgcccctcg ccagagtaga tataaccgcc
    30661 acattcatag cgtcctttat tctctttgac aaaggctctt gcaccagaga aaacttcgtc
    30721 ctgcgttgcc tttggaattt catcataaga agctcgcacg ttatcacatt gccagccaag
    30781 atgtacgctc aatctctgcc agaccacaca ttgttccaac agatagactt gcttgtagtt
    30841 caattccttg tgagtttcgc catactgttt gacatactct aaggataacg ccacatctga
    30901 aatctggtcg gcactcatgc gtgagcttgc gtcagctctg gtcttgtagc cattcttaaa
    30961 gtctgtgttg atgtcgatac agtaagcgtc ctcgccctca acggttaaat gtccctcatt
    31021 aaaggtagaa ccaatcgaac cgtcattcat tactttttca acgataccga cacgctcttt
    31081 tgactccgtc cagtattgtt tactttctgc atgaacgggt gtagtcggta aagcggtaac
    31141 gacagttgca agagctaaga agcccgtaca caatcgtttc aatgtctttt tcataaatct
    31201 aatgctcctt tcattttggg taataaaata gccgtccaat aagaacggct gaaaatagaa
    31261 aaggaacgtc attttaggcg ttccatagtc tataaaatat tcaatttttt tcttgtttca
    31321 atactgatgt gctgtaccat atctttgcat atctaggaaa actggcgtat caaggctcat
    31381 tcaatggtag taaataagta gtaaattgat gtatttgttc ccttgaaaat gctgatagat
    31441 actgtgtttt agcggataat cagattttta ttgtgttttt tacttaaaaa aacaatatcc
    31501 aatctagctg atgaaatttc taaagataaa tctttattca atcaaaagta atataaaaat
    31561 gattaaatag cataatttac atttttagat atttaaaaat aatattcacc aaacaaaatt
    31621 attaagtaaa atgaaatgta tcttataaaa gctgagctat aagtaaatct ttgtaaaaac
    31681 aaatttactt atagcttttt tatgttcaaa aaacacaaat tatatactat ttaatatatt
    31741 ataaactttt atttgttaat actctctact aaatttctaa catctcttat ataaattaac
    31801 tatttaataa ttcaatcata aatctagctc ctgccttaaa accaagctta taaccaagct
    31861 ctctttcaat ctctataact gaattaaata aatcatttaa ctctatcaat acatccattt
    31921 tcttttcttc tgataaattt ttttctagta atttattaac tttcttaatt tctttacatc
    31981 tatcattata atactcatct ttttctagta ctttcctata catgtgttca tttatccaat
    32041 aatcaaaata tatttctata atttcatcat acatgttagt acctccttga ctttcatctg
    32101 gtacttaata tatacttaac ttgtgtagat tagtgtacat taagtaccta atttataaag
    32161 gtagttgtta tatttatatg gcaactacct ttttctaata aatttttcaa tctaaatact
    32221 atatatttta aatcttcatt gcttactatt gaggattata tacttttctt ctaacactcc
    32281 ttaaacaata gtttatatat cacttaataa attatatttt ttcatacctc ctttaccttc
    32341 ttacattcct taaaagaaaa caaacttcct atataccaat aaggtctaaa gcctgattcc
    32401 ccatctctat ttttagcaac tataatttca cttaaattag ctccaaaacc tttttctcta
    32461 cgtcttaatt cttttatatc ctctattccc attgtcttac aataatcaag agcctctttc
    32521 tctgtaggtc tatgaatata tatgactgaa ttagaatcca tatatatctg ttttgaactt
    32581 ctcattactc tatctccttt tggcctttca tccttcaatt catcatttaa ttgagccaac
    32641 tgtataattg gaatattaaa gtctaaagta atctttttta tttgccttga taaatcagca
    32701 aacttagctt ctccaacttc tttaccttcg taatccaaaa tctgaagata atcaattata
    32761 acaacatctg gtttaatact ttttatttct ctcttaattc ctgatattgt attgactgaa
    32821 tcattaatat agagattatt ttcctgattt aaagtactta aagcatttat gtatttaatt
    32881 atttcttctt catttagatt tctattcttc atttttacaa ctgatattcc agttaaagaa
    32941 gctatgtttc taaacaaaat ttgtatatca ctcatttccc cacttataaa aagtactttt
    33001 ttaccttgaa ataccacatt tctcattatc tgtaaggcta gtgctgtttt tcctcttcca
    33061 cttttggctg ctattgtaat taattcacca ctatatagac ctccaatagt ttcatctaaa
    33121 aattttattc ctatatcgat accttcttca atttcatcat ttatgtattc aaaggctctt
    33181 tctgatatag ctgttatact agaatccttc ttaaccaatt tctgattaac ttcattaacg
    33241 tatttttcta attgatatat aagtgtatta tcatctattt cacctaattt gaatttatta
    33301 aatttatcaa ttatatttct atttttatat ttttcaataa ctatctcaat ataatgggat
    33361 acattaccta tagttggaac tattgttgat agttttgtca aataacttat acctccaaca
    33421 taatctataa gccccttttt ctttaaatct tctgtaagag ttattatatc tataggctca
    33481 tcccttttat gtatatcttt cactgaattg aatatatgtt tgtgctcttg atgataaaac
    33541 atatcttcac ttattaatat ctcaatctct tctattttcc tagaatctag taaaatacta
    33601 cctaatatag attgttctgc ttctgtgttc ttaactaaat tactcatatt atccataata
    33661 taaactccct accactttaa ttgtgaatca tctggactat atgcttctgc atctactttg
    33721 acattaattg atttatcttg ataattgcct tctaatatct taataaaatt atttgggctc
    33781 attatccaat caaaatttgc cttccaacct ttttcattgc ttccatttaa aaagctagtt
    33841 ttactaacta tatgaataat ttctaataca gtctctttat catattcttt tattctagca
    33901 ttaattactt tttttctagc ttctgtaagt ttctttactt ttgatagttc tgtacaagta
    33961 atattgaatt tttctataat ttcactgtat attttacttt tttcattatt tacattcttg
    34021 ttagttgtta gttgcttgtt aattgcatgt tgattagtct gttgattttc ttcattagat
    34081 tgatatagct cccagttttc aatagttata agcctatttt tgtttgttga tttatttgtt
    34141 agaaatccat atctttcaaa tttctttaaa gcagttctta cattctgaat acttacatct
    34201 tttgaacaca tatttgctat actttccaaa gatgttatta actgtcctga tttaacttca
    34261 tattctttac ctccataaac ccatttatta ggcttatgat taactgacag taataggttt
    34321 atgagtatat ctctttgttt ggaattacaa tctttatata gtgggctttc ttttatcttt
    34381 ctatgaagct ttatccaacc tagattactc aaatactatc acccacttaa gtctataatt
    34441 ttataccatt tgcattttca ataaaccaat tatctagttt attttttatt atatacactc
    34501 tattatctat ccatatacaa ggaaatcctt tatttttaga atgacataac tgtctaaatt
    34561 ttgattcccc tatgggataa attttagaag cttctttaca agtgataagt tgagctttat
    34621 ctcttctata tgtctgtatc tccttgtacc ctttttcctt ttcttgtgct tcttgttctt
    34681 gcttgacaag tatattcaat gtagcttgta gtgttttaag aacttctaga ttatttgtat
    34741 ctgccataca tcattcctcc ttatcaaaaa ataattcttc tacacttgta tctagttctt
    34801 gagccaattt taacatcaac atattacttg gattttttga tttacctctt tctaaagaag
    34861 ataaataata tcttgagatg ccaacttttt cagctaattc atattgcttt ataccttttt
    34921 caattctttt taatttgagt ttcattgatt tcctcctttc tttcataccc attttgtgta
    34981 tcctcatttc tataaaatat tctaacaccc atttggtgta ttgtcaatat atttaagtgt
    35041 atcaatagac ccatttagtg ttataatgta tttgaggtga aattatgata gataatacaa
    35101 ttggaaaaaa gattaaacaa tatagaaaat ctataaatat gtcaagggat gagttagcta
    35161 ataagattgg ttgctcagtt tatgctgtag ctaaatatga gcaaggccaa agaaagccta
    35221 gtttagatat gataaaaaaa atatctactg cattatcaat tcctataaat aggcttatta
    35281 ttgatgaaag tgatttagat acttcaacaa ataattatac taatttaaaa gaattggatt
    35341 tagttgtaaa aacatttgat aattactcag caaaactcat cggaaataat ataagcaaat
    35401 ttagaaatat aaataatata tctctcaaag aactctcaga aaaaataaat tgttctgaga
    35461 atgaaattat aaattatgag aatggaacaa aaatgcctag aattgaaata ttaaaaaaaa
    35521 tagcacttgc tacttatact aagattgaag acttatttgg atttccatta gaagagataa
    35581 aaatagataa ccaaacaatc ctctatagaa atccccagac tggatatatt atagataact
    35641 ttcttatcga ttttataaat aaagatatta acatagataa tactccagtt tcttattata
    35701 tagataagtc agaatttgat ttagtaattc atatattaaa aaattatggt tatgatgtaa
    35761 aaacagatta taagacaaag tttgaaattg cagataatgc agatacaata aaaccaatta
    35821 gcttaagctt ctctgacttc aaagacttct ccaaaaatct aaattgggtg ataaatggtc
    35881 ttgttgaaaa ttttatacaa gaaaactcta atggtcttat tgaagatgag gatgaagaat
    35941 attagtattg cacattttca aaatatgagt ttacctatta acttttatac attcatagtt
    36001 aataaataaa aattagatat gatatgaaag gagctaaaat catgaataaa tcatatatac
    36061 caaaatacac acataaattc cctactaatc tacctcatgg agataaaaaa tatactttaa
    36121 catacattag aagaatgata agtgaatttg tatatgatat gggtaaccta gaaaataacc
    36181 cctttacatt cccagaagta caaacattac ttgatggtat aaccgtaggt ggacataaat
    36241 taagtgacca aaatcaaata ctaaatataa aatcatcatg ggattatgta ataaaattaa
    36301 gtaacaaaga aaacttatct ttaaataaag atacaatttg ctctatacat aaaatagttg
    36361 caaaagatga agctctaata gttggagatt ttagaaatgg taacataggt atagctggta
    36421 ctactcaata tgaatgcata gaagccactc tattaaatga tttatttgta agcgacataa
    36481 atatcataaa taaaataact aaccccttag aaaaggctat aataataaat ttatggttgt
    36541 cctattgcca attcttctat gatggcaaca aaagaactgc tagacttacc tcaaacctaa
    36601 tacttataag taatgatata ggagttctat caataccagc taaacataaa caagaataca
    36661 acacattaat gctaaacttt tatgaaacac tagaagctga tgaagttata aaattcttat
    36721 tagagaaatg tattacattc tttgatgaat ataactataa atcatataag gagctgttta
    36781 aaaatggcaa ctaaaagagc taatggagaa ggctctatcg gaaaatataa agatggttgg
    36841 cgttcccgta taatgattgg ctataatgaa gatggtaaac caataagaaa agaattttat
    36901 ggtaagactc aaaaggaagt taaagataaa cttgatgctt ataaaaaaca atattattta
    36961 agctctgata tttcagatga taaaataact ttagagcatt ggttttacaa ttggttattt
    37021 gaatatagaa taaaagattt aaaacctaaa tcatttgaaa gatatgaaag catctatcgt
    37081 aattatatta aagatactga tttaggcaat gttaaattaa aggacttaag agtatctcac
    37141 atacaaaaat attacaatag attgcttgat tccaataaaa gtatacctac tataaggcaa
    37201 ataaacacta aactgaaaac ctgcttatca gaagctgaaa aacaaggtct tatacaacgt
    37261 aattattgta ctatggtaaa tataccagtt gagaaaaagg agaataagct tgaaatattg
    37321 actcttgaac aacaaaagag ttttataaaa gctatagatg gacataaatt agaggtatta
    37381 tttttacttg ctcttggtac tggacttaga ttaggagaat tattaggact taaatggttt
    37441 gatattgatt ttaaaaagtc taatttaaca gttaaaagaa cccttcaaag aacatatttt
    37501 atagataaaa ccggaaatag agagcttaaa gtattagagc aagagccaaa gacatcaaat
    37561 tcttacagaa ctgtacctat tccaaaagat gttttaaata aattgaaaga gcataaagta
    37621 aatcaaaatt cggatatatt aaaggctgga gaattatata gaaatgataa ttatgtattt
    37681 tgcaatgaat taggtgcccc tattgatgat aaaagacctt taaggaatct aaaatctata
    37741 ttaaacagct tagatataga gcctataaaa tttcatggtc ttaggaaaac ttatgcgact
    37801 agactttttg agaatgatgt ccctcctaaa actgtacaag ttttaatggg tcattatgat
    37861 atatcaataa ctttaaatat atatactcaa gttatggaag ataagaaaat tgaagctgta
    37921 gaaaagttgg ataaaatatt ttcattgtaa aaatcaaata tataaaaata ctctgatttc
    37981 atatagttaa ttacaaaacc aaaataagga tacaaattaa ttttatatta tcttgtatcc
    38041 ttattcttgt ctagtttttt aaatatttat cttggatagc ctttatgata cattaatcca
    38101 ttttctaaca ttagttcttg ggctaaatat atatgaccct tacagtgcat atctgatata
    38161 ccctcactta taagtttata tgtttcagaa gtatataaat aatccatact ttcttccaag
    38221 ctcttacctg tttgtttaga aaattctaat atgatttttt tatatagcga ttgtaacaat
    38281 gtcttattag cttccataaa agctatacct cctctttcaa atatttgttt atcctatttt
    38341 gactaactat atatttatta gttatttaat tagccataat aattcggcta taagatattt
    38401 ataatctaat ctcaattata taaatctttt tgccaatatt taataatcaa aatattgcac
    38461 ttaattaact atcttataag tattgataat aaaatctgtc ttcttaaata tacttaaata
    38521 aaacttctat aattatatca aagtattaat attcactctc tattgtctct cctacaagtc
    38581 tattttctcc atttagggta tatactcctg atatagttgg ttgtatgtat tttctatcca
    38641 tatatggttc ttctaaaact cttttttctg ctagcatcgt ccaaaataca ctatatcctt
    38701 gttcattaag atattttaag aaaatttcct tattaataaa gaatcctatc tcttcattaa
    38761 gtaattcact actttcaaaa caaacaactt caccataatc attatataga atcgaatctc
    38821 cctgaccata tctcaatttc aacccataaa atagagtact acaaggttta tgccatgtgc
    38881 cagaaaagtc ttcttgaaat atgtcacctt ctcgttcagt agtataacta taagttggca
    38941 ataatacttt ataatccttt tctaagatat tcttatgttc atctatttgt acccactctt
    39001 ctccacaata atatgaattt tgaaaaaagt aatatccatc tgcccaataa tattccttat
    39061 tataaagagt ataattacta ttaggttcag taagccatct acccataaag tcttttccct
    39121 ttagagcatt cactattggt tctagttggt catttttaat tatatagcta ttgattaata
    39181 cccacatgtc tttttcagga tttttatagt tcttattttt aaaatcattt tcctccttcc
    39241 aagtatacca tccattaagt aataaaaagt cttgattttt gtaaacacta tttaccatat
    39301 catttatgct tggtaaatca ctaaaatctc ctaaccactc attattaggt attggagaaa
    39361 attgaaataa ttgattatgt actcttttat tgttattatt atttattact gtcatgacag
    39421 ttggatcaat attcctaata tctggctcaa atgtaccctt acaataaatt tcttttttag
    39481 taccataagc atctgtatac acttctatct tataattatc agcagcttgc gctgcaagtt
    39541 catataatgc aatccattga tatttctttc ctattctttc atatttacta tctcttaatc
    39601 taccattttg tactgttcta tcaaatttac catgtttatt tacatcatat cccatatcaa
    39661 aaactttctt aatagctata ttctttaaat cgttatagtc tagacctttc catttatgga
    39721 aatagtgttg gaacatatat cttccaaaat ctccatacca tcctggttgt ccatctctag
    39781 tatattctac ttccatagaa tttaagatag tgttttgact ccaataataa tctttaaaat
    39841 ctgatgcctt atagtcatac ttatatccct caatttcttc atcagttggt actttaggaa
    39901 aaattgattt atatggtggt tcaacattca tatcttttaa tactgaacta tcaattttat
    39961 attttgcata atctacaata ttttttgcat aatctcttaa taaaatattg ggataaacat
    40021 aatctacatc aaatatttta ttataaacat aaactgccaa tgcctctatc atctcttctg
    40081 attgctctga tacaacacat ccaaatgcaa ctgcatataa tcgttcaata atatatgggt
    40141 catctacatc ttcaaatgtt ttaagtaaat ctattacaat atccattttc cctgttaata
    40201 gatttacaag agcttttgta cttttatctc ttaagtctct atttgaagaa attaaaaacg
    40261 ttgcaatcat catagctgac aatctaattg tttcatttga agtattatca aaaccttcaa
    40321 aatcaaagca ccatcctaat aatcgcttaa tagaagactc ttcttcttca taaacttcat
    40381 taaatatagg tataaagata gcatctctat ctgccatagt attttgtaaa atatatttta
    40441 ctatataatc tgcatttaat gtatgattaa cctttgtaga aattaagata agtacattat
    40501 aaaaatctat tttataataa gatttaccta aaactatttt attaatataa tctagtgttt
    40561 tttcagtaat tgtagatttc ttacgccatt ttagtgcatt gataaatgca ctaataacat
    40621 tataatttgt ttctttattt ttaaaaactt caaaaatttc attaggtata tctgagcctg
    40681 tctcttcaga taaaacaatt gccaatactt ctaatatatc ctcatattct actaaatatt
    40741 tatacttatg tgaaaaatct actaccccaa tttcttttaa ctctttaacc aacaaatttg
    40801 catataaata atcttctaac ttctcatatg tcacatgcat atattcttca tcatttaaat
    40861 tgactgtttt attaactatt ccatttgcta tgagttcatc tattatcaca ttgttaatct
    40921 tatacttact tgcaatattt attataatct ctacactctt gtttgcgggt attaaattat
    40981 tcccaagacc atctccatat ttatatttaa tcatttcatt aagtacttct gtaactacat
    41041 tggcatgtct actataattg catgattcag atattctaag attaagtata ttcaaataat
    41101 ttttataaat atctgtaaaa ctaatagtat ctaagttaat ctcttctcct ttaaaagttt
    41161 cacacataat tctcaaaaaa agagggtttc taaattcttg atttggcatt ggaatattag
    41221 aataaggtat tccataataa tcaaaatact ttttcatagc tttatgttct atactagaaa
    41281 atccttgatg ctctataaaa accaaattct cttttatctt gttattatta tctattaaag
    41341 atggtatata ttcacttctt attgataata ttagtccaat ccatggatat ttctttatct
    41401 gttctacaat accatttaaa taatatttcc aaagttcttt tccttgacct tcattcaatg
    41461 catctataaa taatatcaca cgctgttgct ctttttctcc tatttcatta agtatttcta
    41521 ataattcttc aaactcacac tcaatttcta atatttctaa catatgttta attggtgttt
    41581 tatccaactt aaaatgttgc cctaaaaata atatagactt atgcccatcc atatttcttt
    41641 tctcaatagt atgtgcaatt atatgagatt tccccatacc acctgcacta tgtaataaaa
    41701 tataagggct attaactact tttatttcct tagaatccaa atattttata tatacataga
    41761 tatctttttg agtctgcttc aatttgtata tttcatcttc tcttttttta cttttttcac
    41821 agtcttcatt tataattata tttttaaaat tatttattct atcatcaagt gctgcactta
    41881 ttttttcaaa gtgctttttt aacaacttga tatctaatct ttcattagct ttgtcataaa
    41941 attgttgtat tatttcttct ataactagta catctacttc cacttcattt attttctgtt
    42001 tctttatact tgatagaact ttatctgttt tttcatcaaa acgttgttta aattcttcat
    42061 tttttcctaa tgcattaaac gtattttcta aactcaattc tacattcaat tctggtaaat
    42121 atctatctcc aaggtttttt atctgtatat tattctgatt ttgaaaccat ttctctgtta
    42181 atctacaaga taaattagtt aacctacttt cctttaaaga cctatccttt aatcttctat
    42241 attcatctgg caaaactatt tccaaaattc gttcaagtat tattcttaca cactcatgct
    42301 taccttcagg atacataatg gtttgtatat aatactcatt ttctaactct attcgtctat
    42361 gtttcttttt ctcactacta actggaataa tagcataatg acccgttcct gcctctgata
    42421 cttgctgtaa taattccaac ggtctatcct tttccaaact acatccaaga aataacaact
    42481 ctttacttgt atatacttgt gtaagcatct ttctcaattc atcattacta tatgcttttt
    42541 catattgttc ttttgttaac acaatagatg ttgtaggctc tgtaatatcc ccatgaattt
    42601 tatacaatac taattcatta tttctaagtg cattatttaa tgcttcaaag tgacctggat
    42661 gtgacactaa aactgtttta ttgtgtaatc cataattttt ttctagtact ttatcataat
    42721 tagtagttat aattaatcct gagccaaaaa ttataggaat taaatagaca catttatcaa
    42781 taaaatcgtc tttatctttt gcatccagat aacctgcccc aaaaactttt tttaaatgtt
    42841 tagaaaatct aactttcgcc aattcttcat ataaaaattc tgcttttttc tcataatcct
    42901 ctattttttc aaataaaatt ttatcctcat cttctaaata ttcttcactt gtgttattta
    42961 aaaatgtact ccatgaagga tatatgtctt taaataacat agacatgcct gctccaatat
    43021 atgggactac ataaccatct ttaattgcat ttaccagttc atcaaaattt ttttgattgt
    43081 aaggcttatt aaattccata atttcttcaa atgtcattat tccctcctaa actatctctt
    43141 aataatttca accataattc ctattttata attttattat atcattccat tccatttttt
    43201 tactacaatt ctatgttata gaatgaattc acttacttac atttgttaat aatataaaac
    43261 tttcttttaa gataattaaa atttatactt ttaaattatg gataaaaatg gaatgttata
    43321 acttttaatg tattactaat attaacaatt ttaaaaaata atactattct tattttatgc
    43381 tataattata ttttgcataa ata
//
LOCUS       DAYASV010000018        43134 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYASV010000018 DAYASV010000000
VERSION     DAYASV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.018"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            331..1491
                     /locus_tag="ACGI68_001034"
     CDS             331..1491
                     /locus_tag="ACGI68_001034"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFH0137482.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            1493..2623
                     /gene="glgD"
                     /locus_tag="ACGI68_001035"
     CDS             1493..2623
                     /gene="glgD"
                     /locus_tag="ACGI68_001035"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HFH0137483.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            2652..4094
                     /gene="glgA"
                     /locus_tag="ACGI68_001036"
     CDS             2652..4094
                     /gene="glgA"
                     /locus_tag="ACGI68_001036"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFH0137484.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            4108..6528
                     /locus_tag="ACGI68_001037"
     CDS             4108..6528
                     /locus_tag="ACGI68_001037"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HFH0137485.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRVLL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            6533..8398
                     /locus_tag="ACGI68_001038"
     CDS             6533..8398
                     /locus_tag="ACGI68_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFH0137486.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIRIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            8643..9296
                     /locus_tag="ACGI68_001039"
     CDS             8643..9296
                     /locus_tag="ACGI68_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0137487.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPMEEMKKEKLL"
     gene            9392..9991
                     /locus_tag="ACGI68_001040"
     CDS             9392..9991
                     /locus_tag="ACGI68_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFH0137488.1"
                     /translation="MYPNKEIAIEELEVAGKMNPGPWIKHSYNVANVAKLIAEQCENL
                     NSEKAFVCGLLHDIGRRTGISSVRHIIDGYDYVISKGWDEVARVCLTHSFPVKDIEAD
                     IGNKDISKEQYDFINHYLNNLEYNDYDKLIILCDALADANGFCILEKRFVDTTRRYGI
                     YPFSVERWNKTYEYKEYFEKIINKSIYTLLPNIEKSIYY"
     gene            10581..12056
                     /locus_tag="ACGI68_001041"
     CDS             10581..12056
                     /locus_tag="ACGI68_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0137489.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            12143..12556
                     /gene="speD"
                     /locus_tag="ACGI68_001042"
     CDS             12143..12556
                     /gene="speD"
                     /locus_tag="ACGI68_001042"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFH0137490.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            12586..13437
                     /gene="speE"
                     /locus_tag="ACGI68_001043"
     CDS             12586..13437
                     /gene="speE"
                     /locus_tag="ACGI68_001043"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFH0137491.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            13427..14305
                     /gene="speB"
                     /locus_tag="ACGI68_001044"
     CDS             13427..14305
                     /gene="speB"
                     /locus_tag="ACGI68_001044"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFH0137492.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(14485..14685)
                     /locus_tag="ACGI68_001045"
     CDS             complement(14485..14685)
                     /locus_tag="ACGI68_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0137493.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            15152..16642
                     /locus_tag="ACGI68_001046"
     CDS             15152..16642
                     /locus_tag="ACGI68_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137494.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            16922..18418
                     /locus_tag="ACGI68_001047"
     CDS             16922..18418
                     /locus_tag="ACGI68_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137495.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            18552..19478
                     /locus_tag="ACGI68_001048"
     CDS             18552..19478
                     /locus_tag="ACGI68_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0137496.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTSESYLKS
                     DKNSVRQNGIQRSTKSSTKNTAFKTTILNTGKSDCILVEIGNKVIMIDTGEDKNGKQI
                     VDRLKEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDS
                     LKKADIEPVLLKDNMNIVINGAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGD
                     AEEERLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGV
                     LKILERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            19596..19787
                     /locus_tag="ACGI68_001049"
     CDS             19596..19787
                     /locus_tag="ACGI68_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137497.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            20010..20360
                     /locus_tag="ACGI68_001050"
     CDS             20010..20360
                     /locus_tag="ACGI68_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HFH0137498.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            20621..22126
                     /locus_tag="ACGI68_001051"
     CDS             20621..22126
                     /locus_tag="ACGI68_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFH0137499.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            22516..23613
                     /gene="dinB"
                     /locus_tag="ACGI68_001052"
     CDS             22516..23613
                     /gene="dinB"
                     /locus_tag="ACGI68_001052"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFH0137500.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVEVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            23876..25012
                     /locus_tag="ACGI68_001053"
     CDS             23876..25012
                     /locus_tag="ACGI68_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137501.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            25005..26552
                     /locus_tag="ACGI68_001054"
     CDS             25005..26552
                     /locus_tag="ACGI68_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0137502.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            26887..27753
                     /locus_tag="ACGI68_001055"
     CDS             26887..27753
                     /locus_tag="ACGI68_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0137503.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            27894..29036
                     /locus_tag="ACGI68_001056"
     CDS             27894..29036
                     /locus_tag="ACGI68_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0137504.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            29438..30658
                     /locus_tag="ACGI68_001057"
     CDS             29438..30658
                     /locus_tag="ACGI68_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6080 domain-containing protein"
                     /protein_id="HFH0137505.1"
                     /translation="MSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDLNNTYDV
                     LLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTDFNSAYG
                     FGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNVERFIYS
                     QFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKIGKMLKH
                     LITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLLYSVFYF
                     PGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIIIFNLTLH
                     GIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIVISNVNG
                     FIEILNIGIKSYPV"
     gene            30687..31574
                     /locus_tag="ACGI68_001058"
     CDS             30687..31574
                     /locus_tag="ACGI68_001058"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HFH0137506.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            31608..32237
                     /locus_tag="ACGI68_001059"
     CDS             31608..32237
                     /locus_tag="ACGI68_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HFH0137507.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            32269..32706
                     /locus_tag="ACGI68_001060"
     CDS             32269..32706
                     /locus_tag="ACGI68_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HFH0137508.1"
                     /translation="MGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVYVFSVIGSTL
                     VSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGIILFVGIPLPT
                     TGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            32956..34323
                     /locus_tag="ACGI68_001061"
     CDS             32956..34323
                     /locus_tag="ACGI68_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0137509.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            34523..35797
                     /locus_tag="ACGI68_001062"
     CDS             34523..35797
                     /locus_tag="ACGI68_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HFH0137510.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNVKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            35915..36634
                     /locus_tag="ACGI68_001063"
     CDS             35915..36634
                     /locus_tag="ACGI68_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFH0137511.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            36655..37101
                     /locus_tag="ACGI68_001064"
     CDS             36655..37101
                     /locus_tag="ACGI68_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137512.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     misc_binding    37249..37495
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            37619..39361
                     /gene="leuA"
                     /locus_tag="ACGI68_001065"
     CDS             37619..39361
                     /gene="leuA"
                     /locus_tag="ACGI68_001065"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFH0137513.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTTQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     gene            39318..40595
                     /gene="leuC"
                     /locus_tag="ACGI68_001066"
     CDS             39318..40595
                     /gene="leuC"
                     /locus_tag="ACGI68_001066"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFH0137514.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            40610..41101
                     /gene="leuD"
                     /locus_tag="ACGI68_001067"
     CDS             40610..41101
                     /gene="leuD"
                     /locus_tag="ACGI68_001067"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFH0137515.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            41108..42178
                     /gene="leuB"
                     /locus_tag="ACGI68_001068"
     CDS             41108..42178
                     /gene="leuB"
                     /locus_tag="ACGI68_001068"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFH0137516.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            42541..43056
                     /locus_tag="ACGI68_001069"
     CDS             42541..43056
                     /locus_tag="ACGI68_001069"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HFH0137517.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
BASE COUNT        16987 a         4404 c         7801 g        13942 t
ORIGIN      
        1 tacgaattta acaaattaaa aaataagcat gttcatatga cacaaaggat ttataaaaaa
       61 tgtttttata tttataaaat aaaatttgta ttatttttta accgataatt gaatattaat
      121 aaatactata gtgcttatgg aaaataatgt atacgtcggt aagtccatta tggggtatac
      181 attatatcaa ttgtatagtt gatattattt tatttttatt aaggtattat ttaagcaaca
      241 tttatacacg acatttattg actgataaag tttataaaag taaaaatgca attataaata
      301 ttaattacta gatgagggga gtgtcattaa atgaaaaaag agatgttagc tatgattttg
      361 gcaggagggc aaggttcaag attaggtgtt tttacaaaaa gaattgccaa accagctgta
      421 tcatttggag gtaagtatag aattatagat tttgtgttaa gcaattgttc aaactctggt
      481 attgatactg taggtgtttt aactcaatat agaccgttga tattaaattc acatataggc
      541 atgggaagtc attgggattt ggacagaata aatggtggtg tctatgtttt acaaccattt
      601 atgaatgaaa aagaaggaaa ttggtataat ggtacagcac atgcgatata tcaaaatatg
      661 gactttgtag atacctataa tccagaatat gtattgatat tatctggtga ccatatatac
      721 aagatggatt atagcaaaat gttaaaattt cataaggaaa aaggctctaa agctacaata
      781 gcagtgattg aagttccttg ggatgaagct tcaagatttg ggataatgaa tacaaatgaa
      841 gattcaagta tctatgaatt tgaagaaaaa ccaagtgaac ctaagagcaa tttggcatct
      901 atgggagtat acatatttga ttggaaaatg ttgagaaatt attttaagga agcagaaaaa
      961 aatccagaaa taaattatga tgattttgga aaaaatttaa tacctaaaat gttagaagat
     1021 aatgttggaa tgtatgctta tccatttaaa ggatactgga gagatgtagg aactatacaa
     1081 agtctatggg atgctaatat ggatataata aaatctccag aaacacttga tttagcagac
     1141 cctaagtgga agatatatac aaatactatg gcaatgcctc cacaatatat aggtaaaaat
     1201 gcaaatgttc atagatcaat gatagctgat ggttgtagaa ttttaggtga agtaggtaac
     1261 tctgtattat cacatggagt tgttgttggt aaaggaagta aagtaattga ttcagtaata
     1321 atgccaaatg ttgtaatagg tgaaaatgta actatagaaa aagctatgat aggtgagtgt
     1381 gcaactataa atgataatgt tcagataaag aatgtaaata atgaaataaa tgtagtttct
     1441 gagtatgaaa atatcgaacc tagatgcgta ttaatagaag gaggtcttta agatgagaaa
     1501 tgaatgttta gggataataa atttaaacaa aaaaggagac ccagcaataa acaaacttaa
     1561 ctatggaaga ccaatagcat ctacaccaat agctggaaga tatagaataa tagactttgc
     1621 tttatctaat atgataaatt caggaatcac taaagttgga atattcgcaa aagaaaaata
     1681 cagatcatta acagaccata taggtagtgg taaagattgg gatttaagta gaaaaaaagg
     1741 tggcctatca atttttagtc cagaaaacac taagtataga aatacttact cacatagaga
     1801 aggtgatata tatactatct tagctaatct agattatata gaaaagagtg aagaagaata
     1861 tattttaata gctccaagct atatgctatg caatttaaat tattctcaag cattggaata
     1921 ccataaaaaa tctcataatg atataactat aatatataaa aatgtaaata atgcaaataa
     1981 agattttgct ggtaacttaa ctctaaattt agatagtaac aatagggtaa taaatgtagg
     2041 aaataattta ggtaagttcc ctagagcaaa catatgtatg gagacatata ttatgaagcg
     2101 tgaagatttt gttgaatgta tatataacat tgtaaataaa ggcaattatt gttatttaga
     2161 agaatttatt attgaagaag cagaaaacat gaaaatagga gcctatgaat atacaggata
     2221 tctaaaatgt gtaaattctg ttgaatcata ttttgaaatg agcaaagatt tattaaattt
     2281 agaagttgca gatgaattat tatattctga aagaaagata tttacaaaag aaaagaatga
     2341 atcacctaca atttatacag atagtgctaa agtagaaaat agctttatag catctggttg
     2401 tttaatagaa ggtactgtaa aagatagtat catatttaga aaggtaaatg ttgaaaaagg
     2461 aacagttata gagaactcaa tagtaatgca aaattgtgta ataaagtcaa atgcaaaact
     2521 atataatgca atattagata aaaatacaag tgtatcaaat gggaaagaat taagaggaga
     2581 tgaaaaatat cctcttgtag tggaaaagaa tacaaatata taaataaatt ttttagaagg
     2641 gggaatgact aatgaaggtt ttttatgtaa cagcagagtg ttggcccttt gcaaaaactg
     2701 gaggattagg agatgtttct tatgcattgc ctaaagaatt aaaaaaagaa ggtgtagatg
     2761 taagggtaat aatgcccaaa tattcaacta taccaagtta tttaaaagat caactaaaag
     2821 aaatagctgt gtttagtgta cgtgttggat ggagaaatca atactgtggt cttttagaaa
     2881 tggaattgga tggtgtaaaa ttttacttta tagataatga attttatttt agaagagaag
     2941 atgaaagaaa gagtatctat ggatatggag atgatgcaga aagatatact ttctttacag
     3001 atgcagtttt agaagctata agtagaattg atttttatcc agatgtaata catataaatg
     3061 attggcatac agggatgtta ccattaattc taaaagaaag atatgcaacc cttgaggggt
     3121 ataaaaatat aaaaactatg tatactatac ataatctcca atatcaaggt gtatttgata
     3181 agcatgtttt atatgatata ttggacttac cacaaaaata ctttgataat ggagatatag
     3241 agtattatgg ctcaataaat tttatgaaag ctggtataaa ttttgcagat aaaattataa
     3301 ctgttagtcc tacttatgca aatgaaatac aaacatcttt ttatggcgaa caattagatg
     3361 gattacttag aaaagagtca ggaaaattaa aaggtatatt aaatggtata gattatgatt
     3421 taaatgaccc tgcaaaagat aaagatatat ttgttcatta tgatgtagac tcaattgaca
     3481 aaaaagtaga aaataaattg agattacaag atatattggg actaaaaaaa gattcatcta
     3541 ttccgttgat aggtatagtt tcgagattag tttctcaaaa aggatttgat ttaatagcat
     3601 acatgatgcc agaacttatg agagaagatt tacagattgt cgttttagga actggagaac
     3661 atcaatatca atcaatgttc aactattacg attctaattt ttcagataaa gtatcagcta
     3721 gaattacttt taatgcatca ttggctcaac agatatatgc tgcaagtgat atgttcttaa
     3781 tgccatcatt atttgagcct tgtggtatag gtcaaatgtt agctatgaga tatggttctt
     3841 taccaatagt tagagagact ggagggctta gagatacagt aacaccttat aataaattta
     3901 ctggagaagg caatggattt agttttaaaa attacaatgc tcatgagatg tttttctgtt
     3961 tgaaaaatgc aatcaaagta tttaaggaca aagaaaaatg gattaaatta gttgaaaatg
     4021 caatgaaaac agataatagt tggaaaaaat cagctaagga gtatatagaa acatatagag
     4081 acatatgtga ttaaaggagt ggtttttttg gtaacaataa gcaaaaagaa attcaagcaa
     4141 aaatttgaag taaaaatgta tagcttatat gcacaatcta tcaaggaagc tactgatgaa
     4201 caattgttaa atgtattatg tagtctattg aaagatgaga tagcaaaaaa atgggttgct
     4261 acaaagttag ataagaaaaa agaagtttat tattttagtt tagaattttt aatagggaga
     4321 cagttaaaat ctaatttatt aaatttaaat attgaagaag aagttagaga aggtttatct
     4381 gaattaggaa tcaacttgga tgatttaatt gaagctgaag tagacccagc cttaggaaat
     4441 ggtggacttg gtagattagc agcttgcttt ttagattcta tggcatcttt gaacataagt
     4501 ggtcaaggat atggaataag gtataaatat ggtctgtttg aacaaaaatt tgtaaatggt
     4561 tatcaggtag aagtgcctga taactggctt acagaaggac gatatgcatg ggaaactgta
     4621 agaccaaatg aagcaacaat ggtgaagttt ggtggtgagg tagaattaat caaggaaggt
     4681 agtcatttaa aagtaattca taaaaactac cttcctgtaa tggcaatgcc ttatgatata
     4741 ccaataatcg gataccaaaa ccaatgtata aatactttaa gattgtttaa gagtgaaata
     4801 ccaaagagag attttggtga acttacctca aatgctctaa attattctgg tagctatgaa
     4861 gaggctttaa aacataagta ttatactgag gaaatatcac aggtattata tcctgatgat
     4921 tcaaactatg caggtaagtt gttgaggttg aaacaagaat acttttttgt aagtgcaggg
     4981 atacaagata taataagaaa atataaaaag aacaaattaa atattaataa tttattcgat
     5041 aaggtagcaa ttcatataaa tgatacacat cctaccttat gcataccaga acttatgaga
     5101 gttttgcttg atgaagaaaa tttatcttgg gatgaagcat gggaaataac taaaaaaaca
     5161 gtgtcctata ctaatcatac tataatgtca gaagcaatgg aaaaatggcc tgtaagtatg
     5221 atgaaagaac tattaccaag aatttatatg ataatagaag aaataaatag aagatatgta
     5281 gaggaattaa ataataaagg atatgaccaa gatagaataa aaagaatgag tataatagac
     5341 tgtgacaata tcaatatggc aaacttatgt attgtcacaa gtcatagtgt aaatggagtt
     5401 gctaagcttc atactcatat tcttgaaaca gaggtattaa aagatttcta tcaagatgaa
     5461 ccaaataaat ttaataataa gactaatgga attgcacata ggcgatggct tataagttca
     5521 aatcctcaac ttagcaattt aattacagat ttaattggtg attcatggaa gaaagataca
     5581 ttacaattaa aaaatattga aaaatttaaa aatgattcct ctgttttaca aagacttgat
     5641 gatataaaat acaataataa agcaaatcta gcaaagttta taaaagataa atacgattta
     5701 aatgtagacc caagttcgat atttgatgtt caagttaaga gattgcatgc gtataaaaga
     5761 cagttactga atatatttaa tgtacttcat atgtatcatg aattacttga taatcctaat
     5821 ttgaatcttg acccaagaac ttttatattt ggcgcaaaag cagcaccagg atattatttg
     5881 gctaaatgta taattaaatt tataaattct gttgcaagta ctataaataa tgatgttaga
     5941 gtaaaggaca agttaaaagt agtattttta gagaattatg gagtatcatt agcagaaata
     6001 ataataccag cagcaaatgt tagtgagcaa atttcaacta caactaagga agcatctgga
     6061 acaagtaata tgaagtttat gatgaatggt gctataactt tggctacact tgacggtgca
     6121 aatgtagaga tatgtgaaca ggttggtaaa gaaaatatgt ttttatttgg acttagtgca
     6181 gaacaagtac taaattataa taaatatggt ggatattctt cacttgacct atatcattct
     6241 aatatggata taaaaagggt agtagatgat ttaataaatg gatttattcc taatcttggc
     6301 gaagaaggaa gaagtattta taattcactc actacttaca atgatgagta ttttgtattg
     6361 agagattttg aaaattatgg tcaagctcaa gcagatataa acaggttata tagagataaa
     6421 gaaaaatgga ataaaatgtc tttagttaat atagctaatt ctggattttt ttcttctgat
     6481 agaacaattt ctgagtatgc aaaagacatt tggtttaaaa gggtgtgatt aaatgcaaag
     6541 tgtaatagaa tataactctt gggataaaaa ttttaaggct ccgtttggag ctttaaaatt
     6601 tgatgaggaa ttgaccattt gtgttaaagt aaatgaggga tacaatatca agtctatttc
     6661 cctagaaata aatagagaag aagaaatgag aacaataact ttaaatgaag aattagataa
     6721 tgataaactt ggaaagtgct tttgtggtaa gattgaaaag tttgatggta caggtgttta
     6781 tttttattac tttaaagttg atgttgaaat ggatggtcag ataaagactt tattttatgg
     6841 aaaaaacaga gataatggat attcttgtga atataattat agtgatatta ataaatatca
     6901 aatcactgtt tacaaagatt ttaaagtacc aacatggtac aaagaaggaa tactttatca
     6961 tatatttgta gatagattca ataatggaaa tcgtagtggt aaggttgata atcctaaaaa
     7021 aaatagtttc atatatggaa attgggaaga tacacctatg tacataaagg atagtcaagg
     7081 ggatgttata agatgggatt tccatggtgg aaatttaaga gggataatca ataaacttgg
     7141 atatttaaaa aaattaggtg taagtatact atatctaagc cctatatttg aagcctctag
     7201 taatcataag tatgatacag gtgactataa aaaaattgac ccaatgtttg gggatgaaga
     7261 tacatttaaa gaacttattg ataaagcaaa agaaaaaggt atatcaatag tattagatgg
     7321 agtttttagt catactggag cagatagtaa atattttaat atgtatggaa actataatag
     7381 tttaggggca tatcaatcta aagaatctcc atattattca tggtatatgt ttgaagaatt
     7441 tcctcaaaaa tataaaagtt ggtgggatgt taagacattg ccaaatataa atgaattaga
     7501 acatagctat atggattata taatatatga caatgatagc gttataaata aatgggtaaa
     7561 tatgggtatt aagggctgga gactagatgt tgcagacgaa ttaccaacta agtttataag
     7621 agagttaaaa aaggaattaa aaaaggcaga tgatgactca attcttatag gtgaagtatg
     7681 ggaagatgca tcaaataaga ttagctatgg tcagagaaga agttatttat taggtgaaga
     7741 attagattct gttatgggtt atccttttag aaataacatg ttttctttct taaaaggaga
     7801 aattaattca tatgaacttt gtaatagata tatgcagatt aaagaaaatt atccaaaaga
     7861 atcatttagg tctaatttaa atttaattgg aacacatgat gttacaagag ctaaaacgga
     7921 attaaatgat gatgtagatt tagtgaagct tgctgtagct atacagatga catttgaagg
     7981 agtaccatat atatattatg gagatgaagc aggtctttgt ggaggtgttg acccagataa
     8041 tagaagaact tatccatgga aaaatgaaga cgaagatatg cttaattttt atagagatgt
     8101 tataagaatt agaaataaga ataaactttt aagttctgga aatacagaat ttatatatac
     8161 taataatgat tctgtatttt cttttattag agttaatgaa aataatgata gaatgttaat
     8221 tttagtaaat agaagtgaaa atgtggaaag tatatcatta tgtatagaga gtagttttat
     8281 tgaggaaata cctataaagt atagtcttaa aaatgcaaat agtactatac aaattgaaaa
     8341 taatgagttg aaagtagatt tagactctaa atcatttaga atttttagag taaattaaat
     8401 atcttttttt agaaaaataa atagaaaata aggtgaatgt ggaaagagtt ttgttttatc
     8461 aaaacttttt ctactttttt tgtataaatt atcataagaa ttttagtatt tcaaaatatt
     8521 acaaagaagt ataaaaaata tcataattgt gatattatat aaattagtaa gataaaactg
     8581 aactctctac caaagttgta gggagattta acttttataa tcttaatatg gaggaacgtc
     8641 ttatgaaggt agatccactt acaactcaag tatatgacta tatatcaaag aaaatccaaa
     8701 atggagaata tgaagctaat cagagaatta ctgaatctga gatttgtaaa tctatcggag
     8761 ttagtagaac accagcgaga gaagcattaa ctaggttagc aggtgaaaat ttactagaaa
     8821 aaatacctaa taagggattt gtagtaaaag aatttcagga aaaggagaaa ttagacacct
     8881 attccgtaat tggagtattg gatgcgttag ccggttcttc agcattacaa aatttaactg
     8941 aatcagacct agtaaaaatg gaagaattaa cagaaatgat ggcggtctct ataaagtata
     9001 aaaattacaa tagctattta aaattaagta atgaatttca cgatatatac ataactaaat
     9061 cagataatca ggtgttagta aatctattga actctttaag atataatttt atgtcaaagt
     9121 catatactag tgataatgaa gatgagttat ataaaatgtt gacatacagc aataatcaac
     9181 atattgaagt agtaaaatct atgagggaga atgatttaga aaatgttgaa agaattttga
     9241 gagaacattg gaaaacgata cctatggaag agatgaaaaa agagaagctt ttataaagtt
     9301 tctctttatt ttttatacca ttttatcact atttttttga attattaagt agaaaataat
     9361 aattaattta aacttgagga ggaaaactaa tatgtatcca aataaagaaa ttgcaataga
     9421 agagttagaa gtggctggca aaatgaatcc cggtccatgg ataaaacatt cttacaatgt
     9481 tgctaatgta gctaaattga ttgcagagca atgtgaaaat ttgaatagtg aaaaggcctt
     9541 tgtatgtgga ttattacatg acattggaag aagaactggt atttcttctg taaggcatat
     9601 tattgatgga tatgattatg ttatttctaa gggatgggat gaagtggcga gggtatgttt
     9661 gacacattct tttcctgtta aggatataga agctgatatt ggaaataaag atattagtaa
     9721 agaacagtat gattttatca atcactattt aaacaacctt gaatataacg attatgataa
     9781 gttgattatt ctttgtgatg cattagcaga tgcaaatggg ttttgtattt tagaaaaaag
     9841 atttgttgat acaacacgaa gatatggaat atatccattt tcagttgaac gatggaataa
     9901 gacttatgag tataaagaat atttcgagaa aataatcaac aagtctatat atacattgtt
     9961 acccaatatt gaaaaatcta tatattatta aatcctaagt taataattta atatccagta
    10021 tcaccagcag ttggtcatta tgattgactg cttttattat gcttatatta aaaaaataca
    10081 ctatggtttg tgtatatcaa agttatatgg aatgaaaaga gaatttattt tatcaattaa
    10141 aaaatttaat aaattttata ttaaaattaa aatacaaaaa taggaaaatt atttagtcca
    10201 actaaaccaa gaattttgat atgtgaaata agaataatat aaaagtttag taataaaata
    10261 cttataaatt aaaaatagta aactcaaaag cttactaaaa ctcaaaaaag taaaaaaaac
    10321 aaaataataa aaaataagaa agtttataaa tactaagttt taaaaaaatt aatataaaca
    10381 acaaaaaaat aaaaagttta taaatattga tatagctaat gtatatgttt ttgagtaact
    10441 tcaaaaaaac acttgtaaaa atgctgagat gttaatataa taaatatcat gagtttaata
    10501 aaagtaaaaa taaaaaatag aaataataaa taataaatat taaaaatatt tgaatataga
    10561 gaggagatga atttgaaatc atggatagcg agtttgcaaa tgcagttgaa ttaagctatg
    10621 agtgtgctcc attattagag tcgttaaagg aatactcaga gaaagatata gcttgttttg
    10681 atgtgcctgg acatgtaaaa aatagaggtg tagctatatt aaataaatat ctaggtgaaa
    10741 gtctcatgaa aatggatata aattcatcac caactatgga taatgtatct gcgccaaatg
    10801 ggattataaa aaatgcacaa gatttgctag cacaagcata tatggctgat gaggcattct
    10861 ttataacgaa tggcacaact caagcaatac atgcaatgat tttgagtgtt ataaaaccag
    10921 gtgaaaaagt attacttcca agaaatatac ataaatcagt aataaatgcg ttaatacttt
    10981 gtggaggaat accaatattt atacaaccag aatttgatga aaaactaggt ataagtttaa
    11041 atattactct tgaaaaagtt agaacagaga tagaaaaaga ttgtaatata aaagcattat
    11101 ttttccttaa tccaacttac tatggagttt gtgcagacct tgagagtatt atagaactat
    11161 gccataaaaa taatgtttta gtgttagttg atgaagctca tggtgcacat tttccatttc
    11221 atttagattt acctccttct gcaataagtt tgggagctga tatggtagct gtaagtatac
    11281 ataaaactgg aggagcattg acacaatctt cagcactctt attgaataga gataatgtaa
    11341 gttttgaaaa ggttcttcaa tcaatcaata tgctacaatc aacatcagca tcttatttgc
    11401 ttatggcaag tatagatggt gctagagtta atctagttga gaatggtgaa aagcaactgt
    11461 caaaagcatt aaatctatct aggtatgcaa aatctaaatt aaacaaaatt gatggaataa
    11521 aagttttatc tacagaaata ttgaaacaaa aaggtgttaa atttatagat gaaacaaaat
    11581 tgtgtataaa tgtaaaagaa ttaaatttga ctggatttga agtatacgat ttattatata
    11641 aaaatttttc aattcaagta gaattgggag attcatataa tatactggca cttgtgtcca
    11701 ttggaacaaa taaatcagat atagatagat tagtaaaagc actttctata attgcaaaga
    11761 tttatagaaa agaatcaaca ttaaatgaat ttaatatggt tcaaataaat ccaattatta
    11821 aacttaatcc aagagaagct ttttatgcac caaaggaaag tgtagaaatc aattcatgta
    11881 tagatagaat ttgtggagaa tcaataatgg catatccacc aggtataccg attattgctc
    11941 caggagaatt gataacagaa gaaattatgg agtatattat tttcttaaaa aatagcaatg
    12001 catatcttac agatgtacaa gataaaaact tagatagaat attagtaata aaataaatta
    12061 ctttattttg caagggcaag tatttgttaa gtagagttta tcaaatagaa cttattaaaa
    12121 gtataaagga gttggggttg aaatgaaatt tgaaacacta ggaagacata tactagtaga
    12181 atactataat tgtgatgaaa acgtacttaa agacccacgt cttatagaaa attttatgaa
    12241 tgattcagca ttaaatgcaa aagcgacaat tgtggattct gtattccacc attttaatcc
    12301 ttggggggtt tcaggagctg ttataattgc tgaatctcat ttgacaatac atacatggcc
    12361 tgaatatggc tatgcagcag cagatttttt tacttgtggt gatatagacc catggaaaag
    12421 ttttgaatta ttagagcaac ttttaaagtc tgaaagaagc gagtctacag aaattccaag
    12481 aggcttaact acaaaaatta aaaaatattc aaaaaaagat ttaggtaaga ttactcataa
    12541 accagaagca gtataaaaca tacaagagaa agtgaggatt attatatgga actttggtat
    12601 acagaagaat ggacagaaaa tgtaagattt tctataaaag taaataaaca tctatttgaa
    12661 ggaaagtctc aatttcaaag gatagatgtg tttgatagtg atgaatttgg aaaatttcta
    12721 acaatagatg gattaatgat ggtaacatat aaagatgaat ttatatacca tgagatgata
    12781 actcatgttc ctatggctac aaatttaaac ataaagaaag tattagtcat aggtggaggt
    12841 gatggaggta cagtaagaga attaagccgt tatcctcaaa tagaaaagat tgacatggtt
    12901 gaaatagata aaatggttgt agatgtatct aaggagtata tggatatatg ctcatgtaaa
    12961 ttagatgata aaagagtgag tttatatttt gaagatggtg ttaattttgt taagtgtgca
    13021 catgataaat catatgacct gataatagta gattcaactg acccaatagg accaggtgaa
    13081 gggcttttct caactgattt ttataaggat tgttatagaa tactgactga tgatggaatt
    13141 ttagtaaatc aaagtgaaag tccgtatttt gactttaatg ctaaagagat gaaaagagct
    13201 aataaaaaat taaaacagat atttccaata tcagaagttt atcaagcaca tataccaaca
    13261 tatccatctg gtcactggtt gtttggattt gcttcaaaaa aacttaatcc agtaaaaaat
    13321 caagatagaa atggatggga gaaattaagt ttaaaaacta aatattataa tagtgatata
    13381 catttaggtt cgtttatgtt acctcaatac gtgaaggaga tgctagatga agaataattt
    13441 ctatcatatg aacactttta tgagtatgga caaaaattat gaagaatcta atcttatagt
    13501 atttggtgtt ggatttgatg gaactacttc taacagacct ggggctagat ttgcaagtag
    13561 ctctatgaga aaagaatttt acggtcttga gacatatagt ccttttttag atttggattt
    13621 agaggattat aatatatgtg attatggaga tttagaaatt agtgttggaa gtacagaaca
    13681 agtcttaaaa gaaatctatc aagagacata taagattgtt agagattcaa aggtaccctt
    13741 tatgattgga ggagagcatt tagttacatt accagccttt aaagcagtac atgaaaagta
    13801 caatgatata tatgtaattc attttgatgc ccatactgat ttgagggaag aatataataa
    13861 tagtaaaaat tctcatgcaa cagtaattaa aagaatatgg gatattgtag gtgataataa
    13921 aatatttcaa tttggtataa ggtctgggac aaaagaagaa tttaaatttg ctacagaaga
    13981 aaaacacaca tacatggaaa taggaggaat agatacgttt gaaaatatag ttaatatgct
    14041 aaatggaaag aatatttatc taactataga tttagatgta ttggatgcat ctgtgtttcc
    14101 aggaacaggt acaccagaac ctggtggtgt aaattataga gagtttcaag agatttttaa
    14161 gattataaaa aactctaata taaatatagt tggttgtgac attgtagagt taagccctga
    14221 ttacgataca acaggtgtat cgacagttat agcttgtaaa atcttaagag agttatgctt
    14281 aataatatct gataaaatta aatagagtat tttatataaa tagtatatat tataatataa
    14341 gaagttattt taaaaaagtt agtttaagtt tgacgctttt ctaaagtaac ttcttatttt
    14401 tgtaattaaa aataaatata ataaacaaca aaaaagagcc ctcgatagct ccttttctaa
    14461 aatatatata tttaaattaa ataattatat tctatttacg ttttcagctt gaggtcctct
    14521 attaccttga gttatatcaa agctaacctt ttcgccttct tctaaagttt tgtatccatc
    14581 attttgtata gctgagaaat gtacgaaaac atcatcttct ccctctacag atatgaaccc
    14641 aaatcctttt tcattgttaa accattttac tattccgttt ttcattataa gaaaacctcc
    14701 gcattattaa aaaatacaaa ctaaaaaaat aagtctgttc tattaatata acataaattt
    14761 taaataaaag caaataaaat ttttaaaaaa attaattact tttagaattt aataaaaatt
    14821 gttaaattat acagtgagaa gtattgtaaa atttgacgaa aatgtaaatg tagataataa
    14881 atttaatatc aaaaaatgta acaaaaaggt atgtaagtaa aaagtaaaat taaaatatat
    14941 aataaaaaat attatatatt agattgaaaa gtaaaaataa aaatagtgaa atttcagaaa
    15001 tatcaataat attatataat aaaacaaaaa aacactatat atattcgata aaataaaatt
    15061 taaacatata atattttatg tatatattaa taaaaggaca tattgtgtta taatggtatc
    15121 gtataaaatc atttacgggg gtggtattaa aatgtttcaa tttgaaacac aaatacacaa
    15181 attaagacat gatgttttaa ctgcaatagc tagacttgga aaagaagata accttaccag
    15241 agaagcaatg gataatattc agtatgaaat aataaaaggt gagaaaccta catacagatg
    15301 ttgtgtttat aaagaaagag ctataatatc tgagagagta aatgtaagta tggggttaca
    15361 accaggtcaa gataagatag atttagatac aatggatata gaagaagatg aacaaattat
    15421 atatattttg gaagctgcat gtgacagttg tccaataaat gaatttacag taacagatat
    15481 ttgtagagga tgtctagcac atagatgtaa agaagcttgt aaatttggag ctataacaca
    15541 tgtcggagga atggcctata taaatcatga attatgtaag gcttgtggaa tgtgtaaaaa
    15601 agcttgtcaa tatgatgcca tctcagaggt tgttagacca tgtaagagtg tttgtccaac
    15661 taatgcctta ggatttgata gagaaaatat gaaagctatg atacatgagg ataaatgttt
    15721 aaactgtgga gcatgtatgt cagcatgtcc atttggagca atatctgata agagtttgat
    15781 agctcctgta gctagaaagt tagttcaaaa agagaaaatg tatgcggtgg tagctccagc
    15841 tataacaggt caagttgaag caaatgttac atatggtcaa ataaaaaatg ctataaaatc
    15901 actaggattt gttgatgtat tagaagcagc ttgtggagca gatgcagtta cagttcatga
    15961 aagcttagaa tttgctgaaa gaatggaaaa gggagaagct tttatgacaa attcttgttg
    16021 tccaggattt gttggttata tagaaaaaac atacccaaat catgtaggga aaatatcttc
    16081 tacagtatct cctatgatag ctagtggtag atttataaaa agtcatgaac cagatgctaa
    16141 agttgtattc ataggaccat gtacagccaa aaagagtgag gcagctaaga aagaattaaa
    16201 aggagctgtt gattatgtac ttacttttga agaaataatg gcattatttg aggcatttga
    16261 tatagacttg gctaagtgtt caaatgaaga tatagatgat gcatcaattt ttggaagagg
    16321 ttttggagct gctggtggtc ttacaaaagc tatagaaaat tatcttgctg aaaaaggtat
    16381 tggtgttaat tttgaacctg taaagatttc tggttcaaga gaaatcaaga aaactatgac
    16441 tatggctaat ttaggaaagc tagaaggtaa ttttatagaa ggaatgatgt gtgaaggtgg
    16501 ttgtataaat ggttctggta aattcttatc aggtgcaaag gttaaagcca cttttgatag
    16561 aaaaaattct caatcaacta agaaaagtgt cttagcaaat acaaagataa aagattttag
    16621 taatataaat ttagaaaaat aaagataata caaagcaaca aaaatacata ttgttaatag
    16681 agttgttcta agttgaggtt taacttttgg acaactctat ttttatttat atatttaatt
    16741 attgaacaac taaaataaaa attatataca cttaaaataa aaattacata tataatattt
    16801 tgtatataac ttaacaaagt atatgcctat gttataataa tttatagaaa taaataaaaa
    16861 atacaatata cataatagta gaaatatagc aaaaaggtgt gatttatagg gaggattact
    16921 tatgtttgaa tttgaaacgc aactaaaaag gttaaaacat gaagttttaa cagcagtagc
    16981 aagactatct attgaagaaa atttaacaaa agaaaatata gaaaaaattc catatgaaat
    17041 aattaaagga gaaaaggcga gatatagatg ttgtgtttat aaagagagag aagtagttct
    17101 tgaaagggcg caacttgcat taagtttaac tccaaatagt aaatatggag atataaatcc
    17161 agattcagtt agtttggaca cagatgagca aattatatat atagttgaag cagcatgtga
    17221 tagatgtcca ataaatgaat ttacagttac agagctttgt agagggtgtt tggctcatag
    17281 atgtaaggaa gcatgtaaat ttggggcaat ttcttatata aatgggagag catatataaa
    17341 tcatgattta tgtaaggctt gtggaatgtg caaaagttca tgtcaatatg atgctatatc
    17401 agaagttgta agaccttgta aaagcgtttg tccaacagga gctttggatt ttaatagaaa
    17461 cactatgaaa gcaatgatac atgaagataa ttgtataaat tgtggagcat gtatgtcagc
    17521 atgtcctttt ggagcaatat ctgataagag tttgatagca cctgttgcaa aaaaacttgc
    17581 aaagaaagaa aatatgtatg caatagtagc tccagctata acaggtcaaa tagatagtaa
    17641 tattacatat ggtcagttaa aaaatgctat aaaatcactg ggatttgtag atatgataga
    17701 agcttcttgt ggagcagatg cagttacagt tcatgaaagc aatgaactta ttgaaagaat
    17761 aaaatcagga gataattaca tgacaaattc atgttgtccg ggatttttta agtatataga
    17821 gaagatgttc ccagatgaaa aagatagaat atcatctaca gtgtctccta tggtagcaac
    17881 aggaagatta gtaaaaaaaa tagattctga tgctaaagtt gtatttatag gtccatgtac
    17941 agctaaaaag agtgaggcaa cagagaaaag tttaaaagat tcagtagatt atgtacttac
    18001 ttttgaagaa ctaatggcat tatttgaagc atttaatata gatttatcaa aatgttcaaa
    18061 tgaagatata aatgatggtt cgatttttgg aaggggattt ggagcatctg gtggacttgc
    18121 caaatcagta cagaactata ttgctgaaaa ggggcttagt tttgattttg aacctataaa
    18181 agtatctggt ccaagacaaa ttaaaaagac aatgacaatg gctaaattgg gaagattaga
    18241 aggtaatttt atagaaggaa tgatgtgtga agaaggttgt attaatggag ccggtaagtt
    18301 tttatcaggt gtaaaagcca aaaatacttt tgatagaaaa aatggacaat ctactaaaaa
    18361 gagtgtttta tctaatgata agattaaaga atttgaagat gtaaatttag aaagataatt
    18421 gttttggtac agtcattttt ttattatgcc acaaatatgc ctaaaatttg ccctttttat
    18481 atgtgtattt tacagagtta aggttgctat actataaata tgtaataaat aaaatttata
    18541 ggagcttttg gatgaggaaa atagtattaa aagatatcct gtttttagtt actacattat
    18601 ctataatagt aatattattg cttgtaacaa atataactac taatagtaag ataaaatcaa
    18661 caagtgaatc ttatttaaaa agtgacaaaa atagtgtgag gcaaaatggt atccagagga
    18721 gcacaaagag tagcacaaaa aatactgcat ttaagactac tatactaaac actggaaagt
    18781 ctgattgcat attggttgag ataggtaaca aagtcataat gattgataca ggagaagata
    18841 aaaatggaaa acaaatagtg gataggttaa aagaaaaagg aataaataca cttgactatt
    18901 tgattttaac tcatttagat aaggaccata taggtggagt agatagtgtg ttatctagtg
    18961 ttaaagtaaa gaatattata caagcaaatt ataagaaaga tagtaagcaa tatgatgaat
    19021 atattgactc acttaaaaaa gctgatatag aaccagtgct actaaaagat aatatgaata
    19081 ttgtaatcaa tggtgcagaa ataaacatac atccggcttc aaaaagcaag tacgagagtt
    19141 caaatgatta ttcaataatc actaatataa gctatggagc acataagttt ttatttgcag
    19201 gagatgcaga ggaagaaaga ttgtcagagt ttataaatgg aaatacttta aaatatgatt
    19261 ttgttaaaat gcctcatcat ggaagatatg acaaattaac tgaaacattt ttagagtcta
    19321 tttcacctca atatgctgtt attacatgtt cagagaaaaa agaaccagag gaaggtgttt
    19381 taaaaatatt ggaaagattg aatataaaaa catttttgac ttctaatgga gaggtagtta
    19441 tcaatagtga tggtaagaca ttgagtgtga atcaataatt tttttaaaaa tatctaaaaa
    19501 aatttaaaaa aatcattagc aaatattacc ccttaaacat agtatatttt aggagctgaa
    19561 actccacata caaaatgact aggaggtaac atattatgtt ggatagattt tttggtgaag
    19621 gcggatttgg tggatgtgct tggtggataa taatattatt cttcttattc ttagcattcc
    19681 aagattgttg ggctgaaatg gacttatgtg catggatacc tttcttaata ttattattaa
    19741 tactttgtga atgtggtgac gtttttgatg gaggatgtgg atgctaaaaa aatcaattta
    19801 gatgatttaa taaagcttat ggtgggtata aataaaatac caaaccaatc acaatctagt
    19861 gtttgaaggt agtcggaaat cccccgctac cttctttttt ttattgttaa aatttatttt
    19921 tgaattattt catttacata taataatttt tacgataaag tctgatataa ttaaaaagaa
    19981 aataaaattt atttatagga gatgtaaaaa tggctatagt ggataagatt ttcaatgaga
    20041 taaaacttat ggttgcaaga cttttattgg gaaaaaaata cagtgaatat gaagaaaaag
    20101 atttagtgta taatacagaa gaagaagtta tacttataac tttaaaaaga ttagtttttc
    20161 agggaaatgt aaacgaagca gaagaaattt tatttgataa agctaagagt gtaaatagtg
    20221 aaaatatgca gtatatagca atagaatttt atactatgct tatggaaaag actgatgaag
    20281 aattagaagc tatgaatttt tctaaacaag aagtttatca aggtatagaa gatattagaa
    20341 aagtattgaa tttagaataa aagatatatg aaaatattga aataaattaa tgctaaggtg
    20401 agaatattat acttatatat aaaaatatag tattcttatt ttattagtta tataggtata
    20461 tcttatttac cataaaacta tcttgaaaaa aattaatctt aattgtataa tattactttg
    20521 agtgaaaaag tattaacatt tttttataag atgttttact aaaaaaatat atataaatat
    20581 actgttatca acaagaaatt taatttagtg aggtgttaag atgaaagtag gatttgacca
    20641 caaaaaatat ttagaagagc agtctaaata tatcttagaa agagtaaata attttgacaa
    20701 actttattta gagtttggag gaaaattgat gggagacctt catgcaaaaa gagtacttcc
    20761 aggatttgat gaaaatgcaa agataaaagt attacaacat atgaaagaaa aagttgaagt
    20821 tgtaatttgt gtctatgcag gagatataga aagaaacaaa ataagaggag actttggtat
    20881 cacttatgat atggaagttt taagattaat agatgattta agaggttatg aactagatgt
    20941 aaatagtgta gtaattacta gatttgaagg acaacctgct acaactgtat ttataaataa
    21001 attagaacgt agaggaatta aagtttataa gcattatcca acaaagggat atccgtcaga
    21061 tgttgagact atagttagtg atgagggata tggagcaaat gaatatatag aaactacaaa
    21121 gccaatagta gtagtcacag caccaggacc taatagtgga aaacttggta cttgtctaag
    21181 tcagttgtat catgaaaata aaagaggtaa tgtagttgga tattctaagt ttgagacatt
    21241 cccagtttgg aatgtacctg taaaacatcc tttgaatata gcttatgaag cagctacagt
    21301 tgatttaaaa gatgtgaata tgatagattc attccacctt gaaacgtatg gtgaaatgtc
    21361 agtaaactac aatagagatt tggaattatt tccagtgctt aaaaaaataa tagaaaagat
    21421 aactggaaaa gaatcaattt tccaatctcc aactgatatg ggtgttaata gagttggctt
    21481 tggaataata gatgatgaag taataagaaa agcttctata gaagaaataa taagaagata
    21541 ctttaaaact gcctgtgagt ataaaaaggg acaagttgat aaaggtgctt atgatagaat
    21601 gaaaatgatt atggaagagc ttaacttaaa gccagaagat agaaatgttg tattacctgc
    21661 tagaaactac agtaataaat tgaaagaaag tgccgacaag aatgatactt gtcctgtagt
    21721 tgcattagaa ttagaagatg gtacaatatt aactggtaag ggttctgatt taatgaatgg
    21781 aactgcagca gtagtattaa atgccattaa acatttagcc aatatatctg atgatatgca
    21841 cttaatatca cctgtaatat tagaacctat cataaactta aaaacaaaga ctttagctag
    21901 tagaaatgtt tcacttagtt gccaagaggt gttaactgca cttagtattt gtgctgtaac
    21961 taatccaact gcacaatttg ctatggaaaa gttagcatta ttaaaagggt gtcaagctca
    22021 ttctactact atattaaata gagatgatga acaaacattt agaaaacttg gtatagatgt
    22081 aacttgtgac ccagaatatc cttcacaaaa tctttactat agttaaaatt atataaaaaa
    22141 atatgggctg ttttaaagta atagtttgag cagtccatta caattataat aaaatcagta
    22201 ggctgtcttg tataagacag tcttttgttt ttaaaacatc ccataaatta aatatatata
    22261 ctgttatcca taaggaattt taaatttagt tccttggaat acctattgta aagcttttat
    22321 atagtttttg caagtgtttt taaggatttt ttataaatta tattgaagtt aatggattat
    22381 agatggtttt atttatggaa aatatgaata tataaagaaa ataatgaaaa aataccaaaa
    22441 agaaacataa gtttggtata attaaaaagt aatataaata aaattaatct atttatactt
    22501 taaaaggtga taataatgaa acattataga aaaataattc atatagatat ggacgctttt
    22561 tatgcatcta tagaacaaag agataataaa aaattaaaag gaagacctgt tattgtcggt
    22621 ggaaatccac aaagccgagg tgtggttgca acctgttctt atgaagctag aaaatttggt
    22681 atccattcag ctatgccatc agctgtagct tacaataggt gtccatatgc agtttttgta
    22741 agacctagat ttgatgttta taagagtgtg tcagagcaaa taagagatat attttataga
    22801 tacacagatt tagttgaacc attatcttta gatgaagctt acttagatgt aacaaaaaat
    22861 aaaaaaaata ttgattcatc aattgagata gcaaagcaaa taaaaaaaga tatatttaga
    22921 gaagttggtc ttacttcttc agctggtgtg tcatacaata agtttcttgc taagattgca
    22981 tcagacttaa gaaagccaaa tgggttaact gtaataaccg aagaaaatgc ccaggacttt
    23041 ttagataaat tacctgtgaa taaatttttt ggtgttggaa aagttacttc aaatactctt
    23101 aaaaatttag gtataaaaac tggatatgat ttaaggtgtt taaatttatt tgaacttgaa
    23161 aatatattca aaaaaagagg ttatgaatta tataaatttg ctagaggaat agacgataga
    23221 ccagtggaac caaatagggt tagaaaatct gttggggctg agactacatt aagtcataat
    23281 ttggatattg atgaagaaga aacacgcaac attcttgatg aactttgtga agaagtatgt
    23341 catagattaa aaaactctga gaagtttgga aaaacattaa cattaaaaat taaatatgag
    23401 gactttacaa agataactag aagccttagt ttagaacact atattgacga atataatgat
    23461 ataagaagtg gtgtagataa tttgttgaga aatgttgagg ttaatggaaa acaaattagg
    23521 ctattgggag ttacaatatc aaacctaagt gataaaaaag aaacttataa agatattaca
    23581 ttatttgaat acatggatag tatccaaatg tgagttttgt ttaaatgaaa taacactagc
    23641 tttaatacta tataattgag gctagtgtta ttttatgaat atttttatat tttttgtttt
    23701 ttgacattgt atattatata ctatatataa atactttata tttgtagcaa tattcagaca
    23761 aatagtgacc taaaaatcgt actttgttta cataaataaa atagtatgat ataattataa
    23821 aaacaactta taatattgga gaaggttgct tgttcgaaaa aggggatgat aaattatgat
    23881 agagataaga aatgtaacaa aaaaaatagg aaataatgtt atattagatg atatttctct
    23941 agttgtagaa acaggtacat tggtagtttt gattggttca agtggttgtg gaaagactac
    24001 tactttgaag cttataaaca aattaataaa acctacatct ggtgagatat atattaatgg
    24061 caaaccaatt tctcaggaaa atgaaataga gcttagaaga aaaataggat atgttataca
    24121 gaacacaggc ctattccctc atcttacaat aaaagaaaat atagagctaa ttccacgcct
    24181 aaaaaaggaa aaatccgttg aagaaattga aaaaagaacg ttgcaactac ttgagatggt
    24241 aggacttgat agtgatgaat ttttgaataa gtatccttca gaacttagtg gtggacagca
    24301 acaaagaatc ggtgttgcaa gagccattgc tactgatgct gaaataatat tgatggatga
    24361 gccttttagt gctttagacc caataacgag aacatcttta caagaacaat tattttctct
    24421 tcaagatgaa ttgaaaaaaa ctataatatt tgtaacgcat gatatggatg aggctttaaa
    24481 gatagctgat aaaatttgta ttatgaagga tggaagaata gcacaatatg atactcccga
    24541 aaatatactt agaaagcctg caaatgactt tgtaaaagat tttatcggag aagatagagt
    24601 ttgggataat ccagaatata taaaagcaag agatattatg ataaaaaatc ctattgctgt
    24661 taattctact agaactgtaa ctcaaggaat agaaattatg agaacgagta aggttgatag
    24721 cttactaatt atagatagag caaaaacatt aaaaggtatt gttactgtaa aagatatgaa
    24781 agatattgat gataagtcaa tattacttgc tgatattatg tcaagtgagc ctttgcatgt
    24841 aaatgaaggg gataatttag tagaaatact aaatgtaatg aatcgtaatt cggttggata
    24901 tattccggtt atttctgatg aaaataaact ggtagggctt attactagaa gtagtttact
    24961 ttctgtttta agtgaacaat tcttagaaat ggaggtgagt gttcttggct aattttttta
    25021 actttatact attacaaaaa gataagatta tagagctttt aatacaacat atgagtctaa
    25081 ctgttacatc tattttaata gctataatag ttggtgttcc tcttggaata attatttcaa
    25141 gaatctcatc tcttagaaag tttgtacttg gatttgtaaa cttagtccaa gcagttccgt
    25201 ctatggcact tttgggtctt cttgtaccta tacttggtat aggaagtaaa ccagccatat
    25261 ttatggtagt ggtttattca ttactaccaa ttgtaaaaaa tacgtatata ggtataacaa
    25321 gtattgaccc agtagtatta gaatcagcta aaggtattgg tcttacaaga aatcagacat
    25381 tatttaaaat acaattccct ttagcacttc ctataataat gggtggagtt agaatatctg
    25441 cagttacagc tgttggtttg atgactcttg cagcatttat aggtgctggt ggtttaggat
    25501 atttagtatt ttcaggggtt caaactgtaa ataataatat gattttagca ggggcaatcc
    25561 catcatgtat aattgcactg ttagttgatt atttatttag taaaattgaa gttgcagtta
    25621 ctccaaaagg attaaatcca aaagcaccta agaagaatta tatagcactt aaagtaatat
    25681 ctgtagtggt tgttgtttca atgttgttcg ttgtttttag ttctagtttt tcaagtaaaa
    25741 aagatacaat aactattggt tctaaagatt atacagaaca acttatactt ggtaatgttt
    25801 atgctgaatt agtagaaaaa aatacgaatc ttaaagttaa gaagaattta aatctaggtg
    25861 gttcatctgt tgcgtttaat gcaattaaat ctggtgagtt agacatgtat gttgactata
    25921 caggtacact tttggtaaat gtaatgaaac atgctccgat taaagatgct gatgaagcat
    25981 ataatgttgt aaaagatact atggaaaaag aaaatcagtt aactttactt gaccctcttg
    26041 gatttaataa tacttacaca ttagctatga tgcctgaaac tgctgaaaag tatggaataa
    26101 atactatttc tgatttgact aaatatggta aagaatttac attctcacca actttggagt
    26161 ttgaaaatag agaagatggt cttgtagggc tttctagaga ttatggttta aaattcaaag
    26221 atgtaaaagc aatgaatgga agtttaagat atacagcttt agataataat gaatctcagg
    26281 ttatagatgc atttttaact gatggtctta ttaaaaagtt taatcttaag atattagagg
    26341 acgataaaaa tttctttgta ccatattatg cagcaccatt agtaagagaa gatactttga
    26401 agaaatatcc tgagttagaa aaagttatga atatgctttc aggaaaaata aatgaagaaa
    26461 ctatgagaga acttaactat caagtggatg aacttggtaa atcaccagaa gaagttgctc
    26521 attcattctt agttaaagaa ggcttagtat aaaaaattaa tagtttatat caaaaaattt
    26581 aagagagtgt ctcaaaatga actttttagt tcatgaggca ctctttttta tatgaaatca
    26641 aatatatttt atcatttcaa caataaaaaa gaagattatc tttattttga gacagtctct
    26701 tatttttatg tataccaaaa ccatacctac gatttttatt tataattaag tttcaaatag
    26761 aatgaacaag tacaaccaat tttgaatata aatatagtaa atgtgcttat atacgatttt
    26821 attgtctggt tgattgatat tgatagatag ctttctttat tgaatcgaag ggagggggaa
    26881 tttgttatgg ataactataa gaaagtgaag caagtattgt ggataatttt atttgctaat
    26941 tttgcagtag cattattaaa aatcattatt ggtaatcaaa taaaaagcta tagtatgaca
    27001 gcagacggat ttcattcttt gtcagatgga gcatctaata tagtggggct tataggtatt
    27061 ttctttgcat ctaaaccaaa agataaaaat catccttatg gacataaaaa gtttgaaatt
    27121 ataaccagtt tatttataag tggtatgttg tttgttatag caattaagat aattcttagt
    27181 gcagtgttaa gaatagctaa tccagttgta cctgctataa ctatagaaag tttaattgct
    27241 ttaattatta cactttttat aaatattttt gtatgcatgt atgaatatag aattgggact
    27301 aaactaaata gttatgtttt gatttcggat tcattgcata caagaagtga tatatttgtg
    27361 tctctaggtg tattggttac tttagttggt gttaagttag gatttccagt aataatagaa
    27421 tctatagttc ctattattat ttctgcattc ataatttatt cggcttatgg aatatttagg
    27481 ccatctattg gaattttggt cgatagagtg gctgtagatg aggattatat aaaagaaatt
    27541 gtgtttgaat ttaatgaggt aagggatgtt cacaatatca gaagtagagg tagcaaaagt
    27601 tctatttata ttgacatgca tgtaatggta gacccattta taagcgtaga gcaatctcat
    27661 gatttaacac ataaaattga gaaacaaata caagaagaaa ttaatgaaaa tgctcaagta
    27721 atagtccata ttgaaccttt ttatagtttt taacttagct ataaaataca agttataaaa
    27781 aaatataatt tataaaaaaa atattatacc ataaataatt tataaaaatt atttatggta
    27841 taatattttc tataaagaaa ttaagtctta atatatctaa ggaggaattt acaatgagat
    27901 acgttgaata tggaaagaca gggaaaatgg tttcagtagt tggctatgga ggattaaggt
    27961 ttgatttaga aaaaagtaat caagaaaatg ctgatttagt caaatatgcg tatgaaaaag
    28021 gtattaatta ctttgataca gcaccagggt attgtgatga cagaagtgaa gatatatttg
    28081 gactagcctt tagacaaatg ataaaggaag gtaagaccga tttttatgta tcaactaaag
    28141 aaaagccaaa agcatgtgat acatcagaaa aagctataga ggctgtaaaa aaatcaatag
    28201 aacgattagg cgtatctaaa ataaattttt atcatgtttg gaatattaga aagatggaac
    28261 attacgaact agcaatgaga cctggaggtc aatatgaagg attattgaaa tgtaaagaag
    28321 aaggattgat agaccatata gtattttcat ctcatcaacc aggagatgag gttatagata
    28381 ttctaagtga aaataagttt gaaggtgtaa ctatgggaat aaatatatta aactttccat
    28441 ataggctaaa aggtgccaaa tatgctgttg ataatggata tggtgttgtg gcaatgaatc
    28501 cattaagtgg tggaactatt ccaaagtata ataaagaact ttcttttcta tcaaaagatg
    28561 gcgaaagtac tatagaatca gctttaagat tcaacatagg aatttcacaa ataagtatat
    28621 ctttgatagg gtttaataaa aaacaagata tagatgatgc atgtaaaatt gcagacgaaa
    28681 atagaattta ttcagatgag gatatacatg atattgagac tagattaaat aagaatatga
    28741 acgaaatatg tacaggttgt ggttattgta aagtatgtcc aaaaggtatt aataccccag
    28801 cctatatgct tttttataat gaaaagcaga tgtttaaaaa gagtgatgaa gaaatgacaa
    28861 agttagttta tggcttagga cattggaatt acacaatgaa tagtaaggca aaagctaaag
    28921 aatgtatatc ttgtggaaaa tgtgaagttg aatgtactca gcatttacct ataatagata
    28981 gattaaaaga aattaaaaaa tgggaagaag atggagctaa tagtgttaaa gtctaattaa
    29041 ttttatacta tatttgaaat ttgtatagta tttagtaatg ttttaaaata tagttttaat
    29101 atttaaggag acagattata attttgtctc cttaaattat gctttaattt gttatagttt
    29161 aattccataa gtaacattgt atatttttat ataaagtttt tcaaatattt tacattaata
    29221 ttgcttacaa attctcattt tatacaacac taatctatgt tatataaaca atacttgact
    29281 gattataaca tcaataaagt aaatattgtg gttgtaaata aagagttatt caattacaat
    29341 aaaacaagta agaacattat gaagatggct attaaatata aggaataaat tgtaggtgtc
    29401 agcaaatcct atataatgaa tggatggtga tatattgatg agtaaaatat taaaatataa
    29461 aaacgagatg tttttgttta tattagttgt aataacttac ttaataataa ctaagatatt
    29521 tttttctaag actactatat tttatgattt aaacaatact tatgatgtat tgttagatac
    29581 agatacagga gttttattca atttaaatgt atttgctata agtcaagaca atagtaagca
    29641 tatattgttt tcagcaatta tatcaatttt tgcatatcca atatacctat tttgtacttc
    29701 aatagcaaat cctgggacaa cagattttaa tagtgcctat gggtttggac taatttgtct
    29761 acaaataata actagtgcta tgtcaataac tttagtcttt aatcatatta aaaagataaa
    29821 aatgcagaga ttaactttaa ttttacttac tatgataatg atattttctt ttccacagtt
    29881 atttatgact ttaaatgtag agagatttat atatagccaa ttttcattaa tatttttcat
    29941 agttattgca aataagatga aaggaaaaaa ttcttatttg gtagaattag cagctattcc
    30001 tctatttgga ataactatta gtaatatata tttgtatttt tttaatatga tatttgagtt
    30061 taaattaaaa ataggaaaaa tgttaaaaca tttaattaca tttatcctaa tggcatatat
    30121 ttgtgtagtt tcaacgaagt cttatgagag ttttatgaac cttggaaatg taatacagta
    30181 tgatacgaaa tttataagtg gagaaccaat tttggaaaag attgcaatga ttatagagag
    30241 attattgtat tcggtttttt attttcctgg agcaagtatt aaaaaagggt tatttttgca
    30301 aaatggagaa gttgctacaa taccagtaat tcttacatta ttagctttat gtttttgtgt
    30361 tttatcagtt attgaaaatt cagaaaaaag agttcctaag ctatgtatgg gaataattat
    30421 attcaatcta actttacatg gtatagtagg atataatcta gtcaattcaa gtattatggc
    30481 tataaatttt agttttgcag ttataatttt attggcttac tttacgaaag cccttagaaa
    30541 aaatgaaaag aatatgtata atatatttct atcgctgtta ttggttacta tagtaatcag
    30601 taatgtcaat ggatttatag aaattttaaa catagggata aaatcatatc ctgtttaaaa
    30661 aataatattg aaagggtagt aagtttatga aacagaccaa tacaagtata aggcttctta
    30721 ctaaaatttc agattatata aagttaatga gaataaagca atatataaag aatttctttg
    30781 tattttcagc tataattttt tcaaacaaca tactaaatat aaatttattt ttaaatacgt
    30841 ttattgcgtt tgtgtgtttt tgcctaatgt catcatcagt atatgcactg aatgatgcta
    30901 tagacatgga taaagataaa aaacatccta aaaaatgtaa tagaccagtg gcaagtggaa
    30961 gaataagcaa aaaaagtgct aatatactat ttgtagtatt agcgttaatt tcagtctgtt
    31021 tatcaacagt tgttagtttt aatctttcta taattctcag tctctatttg ataaacaata
    31081 tattatattc tcttaaacta aagaatataa tactattaga tgtgttttca atatctcttg
    31141 gttttatact tagggtttat gcaggatgtg tagccataag tgttagttta tcaaattgga
    31201 taattttatg tacatttttc ttatctctgt acttagctct tggaaaacgt aaaaaagaaa
    31261 tagaaactct aagagatgat gcagttgaac atagaaaaat attggaagat tatgacatag
    31321 aaaatctaaa tcaaatgatg atagttatat taagttctac aattgtatgt tatgcattgt
    31381 attctacaag taatccagaa aagccacata tgatatttac aacaatattt gtagtttatg
    31441 gtgtacttag atataattat ataattaaca ctacaaatga aaataatcct actgatattg
    31501 tcctaaaaga taatgcttta aaaataaacg taatcttatg gataattaca tgtttaataa
    31561 tattgatttt ttagtgatat ttttagtgat aggatgggga aatacttatg aaaaaaagtg
    31621 tattattact tgatgtagat tatactgtta taaatactga tagcatgata gattttttta
    31681 tttactcttt aaaaaataaa acctttaaaa ctattataaa gttgccatac attattttta
    31741 ttctatttat gtatttaatt agaatgatac ccttgaagaa ggcaaaagag gcaatctttt
    31801 atccaatagt agattttagt gaagaagatt taaaaaagtt ttttgatgat tgcataatga
    31861 aaaaaattaa cgaatctatg aaaaaggtta tttacaaaaa taaagaagaa gataatgtta
    31921 taattatgat aactgcatct ccatatgcat atatgaaata ttttaagtat tatggtcttg
    31981 cagacgaggt tataggcacg gagttttttt atgaaaattc aagatataaa aatcaattca
    32041 ttggagaaaa ctgtaaggga attgaaaaag taaaaagaat aaaggctgta ttaggtaaac
    32101 ttgggataga gattgattat gaaaattcat atgcatattc agactctaag agtgatttac
    32161 caatgctttc attgactaaa aatgcatttt tagttagtaa aaaggatggt gaggttatag
    32221 agcaaataaa ttcataactt aatgtcactt agaataggag ggaatagttt gggatattta
    32281 cacatattaa ttttatctat ggttcctgta acagaattga ggggagccat tcctataggt
    32341 attgcaatgg gcttaaatcc tatatgggtt tatgtattta gtgtaatagg ttctacattg
    32401 gtatcgattc ccttgatatt gacattcaga catgtattac aatttttaag agggaagaaa
    32461 ctatttaaag ggattgctga tgtaattgat agaaaaataa actcaagaat gaagaaatta
    32521 aaaagtgttt cgataattgg tataatacta tttgtaggta taccactgcc aactacagga
    32581 acatggactg catctgctat agcttccata ttaaaaatga gaataaaaga tgcttttatg
    32641 ggagtatttc ttggcaattt gttatcaggg gttatagttt cagctctttc tttacatata
    32701 atataaaact atctaagtaa tatacttgga tagttttttt atgagtatat gtgaaaaatt
    32761 tttaataata aataaaaaat gagataaagt atttaacttc agttagttct aaaatgttta
    32821 ctatggcaaa agaaatatag tatagtatta cttgtaggga aagtactcta aatttgtctg
    32881 ctaataaaat gaaattttta tatagaataa aattttagtg atacatttta attattggat
    32941 tgaggaggtt ttaaaatgga aaattcaaac actaacttag gtagtgaaag tgtgggtaaa
    33001 ttattattta aacttgctac acctgctatt atcgcacaaa ttgtaaatgt tttatacaac
    33061 atagtcgata ggatttttat tggtagaatg gaaaatggag aagttgctat ggctggtgtt
    33121 ggagtggcat ttccaataat aattataata actgcatgta gttatttaat cggtatggga
    33181 ggaggtccat tagctgctat aaaaatgggt gagcaaaaca atgatgaggc agagaaaata
    33241 atgagtaaca gtttttctgt attggtgatt ttagcaatac ttttgactat aggttttaag
    33301 attggaaaag agcctttact ttggatgttt ggagcaagtg aatctacaat tggatattca
    33361 atggactatt taaatattta tttaataggt acagtgtttg ttcagatttc aatgggtatg
    33421 aatactttta taaataccca agggtttgct acaactggaa tgatgacagt tgctataggt
    33481 gctttaataa acataatact tgacccgatt tttatatttg gctttaatat gggtgtaaaa
    33541 ggagctgcat tagctactat aattgcacaa ggcgtttctg ctatttgggt acttatgttt
    33601 ctctttggaa agaaaagtat acttaaaata aaaaagaagt atatgatacc aaaagcaagt
    33661 atcatcttac cagtgcttgg cttgggaatc tctccattta taatgcaatc tacagaaagt
    33721 ttagtactta tagccttaaa tagtaaatta caaatgtatg gaggagattt agcagtaggt
    33781 tcaatggcca taatgagcag tataatgcaa attcttatgc ttcctaatat gggggttaca
    33841 caaggagcac aaccaataat aagttataat tatggttcag gtcaacttga tagagtaaaa
    33901 aagacattta agttgtgttt attgtcatgt tttacatatt ctactatttt atggctttta
    33961 ttaatgatat ttcctgcatt ttttgttagt atatttaata agaatccgca gttacttagt
    34021 atgactagtt ggagtataaa aatatatttt gctggtgctt ttatgtttgg gattcaaata
    34081 gcttgtcaac agacattttt agcacttgga aaagcaacaa tatctttggt tttagctcta
    34141 cttagaaaga ttgtactttt aataccttta atatttatac ttccaacatt ctttaatgaa
    34201 aaactttttg cagtaatttt agcagagcca gttgcagata taacagccgc tactataaca
    34261 gcaatttctt tctttatatt ttataaatgt tttctatcaa aacctaaagc aataaaagaa
    34321 taagatatac aatataaatt ttttatgaaa atattttatt agataaaaat tagaatttgt
    34381 attttaattt aataagatat tttctataat gtataaatat atgaagttga atttatttta
    34441 aaaattatag taagaaaaat gtaagtattt gcaatattaa tggtataata tttacagata
    34501 tgataatcaa taagaggaaa ggatgtttat aatggaaaat gatttaacaa tgcaagaatt
    34561 acttgaccag caagaacaag tattcagcaa agtgaaagtt ggagaattga caactggaaa
    34621 aataactgca gtgagaaatg atgaagtaca attaggatta gactatgggt ttgatggaat
    34681 aatacctata agtgaattga acatagaaaa aaatcaatat atagaagata tataccacat
    34741 aggtgatgaa ataactgctg taataacaaa agtatctcaa aaagatggta caataacttt
    34801 atctaaatta caacttgaca aaagaaatga ttttgccgag ttacaaaagg cttatgatga
    34861 acatagaata ataactgtaa atgttgaaaa gaatatagat aaaggtgtat ttgctaatta
    34921 caatacatat acatttttta taccaatttc tcaacttgat acaaaattta ttacagatac
    34981 atctaaattt gttggtctta acttagaagt ttatataaaa gaattggatg ttagaaaaaa
    35041 tagacttgta gcatctcata gagatgtatt acaagagcgt ataaataaag agagagaaga
    35101 aagaagagct caaattaagg cagaaaaaga agctgaaaga gcaagaatta agcaagaaag
    35161 agaagaagaa aaagctcgta tcaaagcagc taaagaagac ttatttaatt ctcttgaagt
    35221 tggacaaaaa agagatggta aggtgacaaa gataatgcct tatggtgcat ttgtggatat
    35281 tggtgggatt gaaggattag cacatataaa taatttagct tggacaagag ttgaaagtgt
    35341 tgaagatgtt gtttcagagg gacaagaagt agaagtttat gtgttggatg taaataaaga
    35401 gactaaaaaa attgccttgg cattaaaaga cataaataat gacccttggg atttaatagc
    35461 aaaagaagta caaattgatg atgttgtcaa tgttaaagtt cttagaataa tagaaaaagg
    35521 ggcttttgtt caaattaaag aaggtgtaga tgcgtattta ccaatatctg aattgagtga
    35581 tactagagtg gctaaagtta ctaatgttgt taatattgga gatgaggtta aagttaagat
    35641 tcttgatttt aaaccaaaga ctaagagaat gttggtttca ataaaagaag caactagaga
    35701 gccagaagag gatataacag aatacctaga agtagaggaa tctttaggaa gtattggaga
    35761 attatttaaa gataaattta aagatttaga agtgtaaaat aattgaaaat ggggctgttt
    35821 taaaacgtta cactgtctat tatgacagct ttagttttga aatagccctt tagctatttt
    35881 cacgaattta tgtaaaatat taggaggaaa tttaatgaaa aagtctatta tggatgtaca
    35941 ttgtcacact ctaataagtg gacatgcaca tagtactttc aaggaaaatg tcgaagaggc
    36001 gagtaagaag aatataaaat acttaggaat atcagaccat ggacctaata tgccaggagg
    36061 accacatcca ttctattttt ataatttgca tttgcttcca cgtgaagtgc aaggcgtaaa
    36121 gattcttaga ggaatagaag gaaatataat ggattataat ggtaatttag atgttcaaga
    36181 agatatgttg caacatttgg attatataat cgcaagtttg catagacctt gtatagcttc
    36241 tggtacaaaa gaagaaaata caaatgctat tttaaaagtt atggataaac ctaaggtaaa
    36301 aataattgga catccagatg acagtaggta tcctttagat tatgaaccta tagtaaaaaa
    36361 agctaaagat aaaaatatcc ttttagaaat aaataattca tcattaagtt caaactctca
    36421 tagaacgggt acttgggaaa atgtaagtca tatgttgaca ttgtgtaaaa catatggtgc
    36481 aagagtaatt cttggtactg actctcatat atgttattct ataggagaat ttgaaaatgc
    36541 agaaaaagtt ttagaagcag tagattttcc agatgaatta gtcataaatt atcatgaaga
    36601 tgaaattata gaattttttg atataaattt ttagaatctt ggaagtgaaa aaatttgagt
    36661 aaaaataagt ttatctatat gaattattat gacaatggtt attttaaaaa aggaatgact
    36721 atatatatgg ttttagcact tataatttta gctacaattt ttatatattc aacgacaata
    36781 ttatatattt tatgtataat aacagcagtt tttctattta aacaaggtcg tgatttttca
    36841 aataaatatg agggaaaagt atatattact ctcgataatc attcaatatt aataaataat
    36901 caatgtatat tcagtataaa atctaaacaa agtcaaaagt ttaattataa gataatagaa
    36961 aaaatagaag tagtaaaaaa tatattaaat atatacactg acgaatctaa ttataaaatt
    37021 agattaaggg cattgagttt ggaagatgaa aaaaaattat tgaatatcat agatgaaaag
    37081 atgaaaaagt ttaaggcata atatcatact gttttcaata gttagcatgt tttatagaat
    37141 tgcttatcaa aaataaaaaa ttgagataat tattttttgt gttgacaata cattttaatg
    37201 tgtatataat aactattaaa tcaaaatatt tagaaaattt aaatgattaa aaaagtgatg
    37261 aatagaagta gtaagaacaa tgaaactttc agagagctaa aggatggtgt gatttagtag
    37321 ttgatagttc tgaacttgtc taggagctgt tttctaagat ttaaatctta gaataatcgg
    37381 agtaattcca cgttatagga aatatagtca ttataattat tgtattggct attactaagg
    37441 gagcttttta gaaagctaaa tagagtggta ccgcgagcaa aacctcgtct ctaattttat
    37501 tagagatgag gtttttttta ttctaatgat aaaattgaca tttatcctta tgaattgtgc
    37561 aggattgcgt cgatatgagg tatactgaat acaaaaaaca gaaaaagggg gaaataacat
    37621 gaagtgtgga aagtataaaa aatatgataa aatgcaaata gtaaatcgta aatggccaga
    37681 taatgaaata tttaaggctc caatttggtg tagtgtcgat ttaagagacg gaaatcagtc
    37741 actgccaaca cctatgagtg taaatgaaaa agtcagaatg ttcaaaatgc ttattgatac
    37801 aggatttaaa gaaatagagg taggatttcc atcagcatca aatacagagt atacattttt
    37861 aagaaaatta atagatgaga atatgattcc agatgatgta actatacaag tacttacaca
    37921 atctagggct catcttatag aaaaaacatt tgaatcaata aggggatgta aaaaagcaat
    37981 tatacattta tataactcta catctgttct tcaaagagat gtggttttta atatgagtaa
    38041 acaagaaata atagatatag cagttgaagg agcaaaatta tttaatgaag aagtaaaaaa
    38101 atatccagaa acagaattta cttttgaata ttcaccagag agttttacag gaacagagat
    38161 ggattatgca cttgaaatat gtgaggcagt gatagatgtt tggaaaccaa ctactcaaaa
    38221 aaaagtaata ataaatcttc catcaacagt tgaaatggca acaccaaacg tatatgcaga
    38281 ccaaatagaa tggttttgta aaaatataag ttgtagggat tctattatac taagtttgca
    38341 tactcacaat gatagaggaa cttgtacggc tgcaagtgaa cttggattat tggctggtgc
    38401 cgatagactt gaaggaacac tatttggaaa tggtgaaaga actgggaata tggatatagt
    38461 aaatgtggga cttaatcttt atacacaagg aatagaccca gagctagatt ttagcaatat
    38521 agataagata ataggtatat atgaagattg tacaaaactt atggtacatg atagacatcc
    38581 atatgcaggt aatcttgtac attgtgcttt ttcaggttca catcaagatg ctattagaaa
    38641 aggcatgata gcaatgaaaa acagagataa tgattactgg gaagttccat atttaccaat
    38701 agaccctcat gatattggaa gagaatacaa agaaattata aggataaata gccaatctgg
    38761 aaaaggagga gctgtttata taatggaaac agattatgga tttatgatac ctaagaatat
    38821 gcattctgat tttggaaatg tagtcaaaat ggaatctgat agaattgggg aagaacttag
    38881 tagtgaagca atatttaatt tgtttaagaa agaatatatt gaagttgaga gcccttataa
    38941 agttaagaag tataaaataa aatcaatgga tgaattaaac tatgaaaatg atgattctaa
    39001 tgatactaac atgattgaga tgactgcaag aataagttat atgggaaatg aacaaaggat
    39061 tgttggtata ggaaatggac ctgtagattc atttaataat gcactaaagc aatgtggcat
    39121 gaaagattat aaatttagat attactggga acatgcatta gaggaagggt cacattcaag
    39181 aggggtagct tatgtaggaa tagagcacaa taatgaagtc tattttggtg taagtatatc
    39241 ggaaaacata aatacagcag ctattaatgc acttatgaat gcaataaata aaagttatat
    39301 tgaggaggaa ataaaaaatg gggatgacta tgacgcagaa aatattagcc aaacatgcta
    39361 aattaaatga agttaagaaa gggcaattaa tcgaagcaga tttagatgta gttttaggta
    39421 atgatataac atcaccagta gccattagag aatttgaaaa attaggaatt gaagatgttt
    39481 atgataagac taaagtagtg atggttttag accactttac acctaataaa gatataaaga
    39541 gtgcagaaca atgtaaattt acaagaagtt tcgcaaaatc aaaaggagtt gtaaattttt
    39601 ttgatgtagg agatatggga atagagcatg ttcttcttcc tgaaaaaggt atagtaactg
    39661 ctggagatgt aattataggt gctgactctc atacttgcac atatggagca ttaggagcat
    39721 tttctactgg tgtaggttca actgatatgg gagcaggtat ggctacagga aaatgttggt
    39781 ttaaggttcc tggagcaata aaatttgttt tgaagaataa accaaataaa tggataagtg
    39841 gtaaagatat aatacttcat ataatagggg agataggtgt tgatggagca ctttacaagt
    39901 caatggaatt ttgtggagat ggagtagaat acttatctat ggatgataga tttactattt
    39961 gtaatatggc aatagaagca ggagcaaaaa atggaatttt tccagtagac gataagacta
    40021 tggaatatat aaattcacat aagtgttcaa ctatgacaaa agatgtaaat atatatgaag
    40081 cagatgaaga tgcagtctat gatgaagtat acgaaataga tttagctgaa ctaaaagaaa
    40141 cagtagcctt tcctcatctt cctgaaaata ctagaacagt agatgagata gataaggata
    40201 taaagataga ccaagttgta ataggctctt gtacaaatgg aagaatatca gaccttgaag
    40261 tagtagctga gataatgaaa ggcaaaaaag tagcagatgg agttagagtt atgatatttc
    40321 ctggaactca aaaagtatat cttgaagcta tagaaaaagg gtatattacc acttttatag
    40381 aggcaggagc agcagtttct acaccaactt gtggtccatg tctaggggga catatgggta
    40441 tacttgcagc aggagaaaaa tccatttcaa caacaaatag gaatttcgta ggaagaatgg
    40501 ggcatgttga ttcagaggtg tatcttgcaa gcccagctgt agcagcagca tcagctataa
    40561 ctggaaaaat atcaaaacca tcagaaataa tttaagggag gtaaaattta tgattgccaa
    40621 tggaagtgtt tttaaattcg gtgacaatat agatacagat gtaataatac cagctagata
    40681 tttaaatata gctgattata aagaactagc aacgcattgt atggaggata ttgatgataa
    40741 atttatttct aaggtaaaaa aaggagatat tatagttgca actaaaaact ttggttgtgg
    40801 ttcatcaaga gaacatgcac caattgtaat aaaagaaagt ggagtatcct gtgtgattgc
    40861 ttctactttt gctagaatat tttttagaaa ctctataaat atagggcttc ctatacttga
    40921 atgtgaagaa gctgccaata acatagatga aggggataat atagaagtag atttttctac
    40981 aggtgtgata aaaaatataa caaaaggaaa agaatataaa gcagaaccat ttcctgagtt
    41041 tatgcaaaat attatattaa atgaaggctt aattaactca ataaaagcaa atagggggta
    41101 gtgaagtatg aattgtaata tagcagtcat aaaaggtgat ggtgttggac cggaaattat
    41161 agatgaaggt ataaaggttc taaataaaat ttgttgtaaa tttaatcata gattcgattg
    41221 tgaatatgta ttagctggag gatgcgcgat tgatgaaact ggagaacctc tacctaataa
    41281 aactgttgaa acttgtcgaa aaaacgaagc tgtattgctt ggtgctgttg gaggaccaaa
    41341 gtgggataaa tgtaagggag ataaaagacc agagtctggt cttttaaaat taagagagtc
    41401 attgggttta tttgcaaatt taagaccagc tacaatgtat gaaagtataa aagaagcttc
    41461 accacttaga acggatatag ttgagaaagg cattgatttt gttgttgtaa gggaattaac
    41521 agggggcatt tattttggag aacgtggaag aaaaatcatt gatggtatcg aaaatgctta
    41581 tgatgtagaa atatataacg aaaatgaaat aagaagaatt ggtaagagag cttttgaaat
    41641 agccagaaat agaaataaaa aacttataag tgtagataag gcaaatgtgc ttgagagttc
    41701 aagactttgg agaagtataa tggaagactt agctaaagag tttgaagatg tagaactgtc
    41761 acatatgtat gttgataatg ctgctatgca agttgtaaaa gaccctagcc aatttgatgt
    41821 aatagtgact aataatatat ttggagatat aatatctgat gaagcatcta tgattacggg
    41881 ttctataggt atgttaccat cagcatcact tagagaagat agttttggta tgtatgaacc
    41941 aatacatgga agtgcacctg atatagctgg aaaagatatt gtaaatccaa ttgcgactat
    42001 tctatctgta agcatgatgt taagacatag ttttaactta gaagaagaag ctaagtgtat
    42061 tgaagatgct gtacaaagtg tactcaataa aggttataga actattgata tctataatgg
    42121 tgtgggaaat gttgtaggaa caagggaaat gggcgaatta atagtaaatg aaatttagaa
    42181 ttatagttta atttgaataa tataatttaa atatataact tgtagaatta gtaaaaatct
    42241 attgtagtat aatttgtttt atgaggggtg tctcaaaata aactttatta gttaatgaga
    42301 cactcttttt tatattaagt caaatatatt tttactattt taataacgaa aaagaggttt
    42361 atatctactt tgagtttcta ttttgataaa gttatttttt ggaaaataat actttaaatt
    42421 aaacttatca tattgaagta gcctattttt attattagaa gtttgcaggg attttattaa
    42481 tattatataa tatattttag aattatttaa tattaagcga ctactaatgt cggttaggag
    42541 atggattttt tggcaaagta taaagttttg aagaagcaaa atagtagtaa atcttgttta
    42601 gtttgtggaa cacaaaatga gctaggactt aaagcagatt tttatgaact tgaaaatgga
    42661 gagcttgtat caatttgtaa tacaaaggat tggcatcaaa gttatcctgg gagagttcat
    42721 ggaggtatgt ctgctgcaat attggatgag actattggaa gagctgtaag tataaatgat
    42781 gaccaaatat ggggagtaac agtatcttta gagttgaaat ataagaaacc agtaccaaca
    42841 gatgctacta taaaagttgt tggaagaata actaaagaaa acagaaaatt atttgaaggt
    42901 acaggagaaa taattctacc aaatggagat atagcagtga cagcaactgg aaagtatatg
    42961 aaaatgccta ttggacaaat tgcagaaggt gatttttcta atgaagaatg gttttttgaa
    43021 gaatcgaaag aaaaagtgga atatattgaa ctttgattaa ataaaaattt tatattttaa
    43081 aacataaatt ttatatttag aaaaaataaa attcaaaaat acaataaaaa taca
//
LOCUS       DAYASV010000019        41235 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYASV010000019 DAYASV010000000
VERSION     DAYASV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.019"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(344..1411)
                     /gene="rsgA"
                     /locus_tag="ACGI68_001070"
     CDS             complement(344..1411)
                     /gene="rsgA"
                     /locus_tag="ACGI68_001070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFH0137518.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1408..1809)
                     /locus_tag="ACGI68_001071"
     CDS             complement(1408..1809)
                     /locus_tag="ACGI68_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HFH0137519.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2606..2702
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2756..2932
                     /locus_tag="ACGI68_001072"
     CDS             2756..2932
                     /locus_tag="ACGI68_001072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137520.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3342..3980
                     /locus_tag="ACGI68_001073"
     CDS             3342..3980
                     /locus_tag="ACGI68_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137521.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4453..5811)
                     /gene="rlmD"
                     /locus_tag="ACGI68_001074"
     CDS             complement(4453..5811)
                     /gene="rlmD"
                     /locus_tag="ACGI68_001074"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFH0137522.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(5986..7746)
                     /gene="pyk"
                     /locus_tag="ACGI68_001075"
     CDS             complement(5986..7746)
                     /gene="pyk"
                     /locus_tag="ACGI68_001075"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFH0137523.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(7798..8757)
                     /gene="pfkA"
                     /locus_tag="ACGI68_001076"
     CDS             complement(7798..8757)
                     /gene="pfkA"
                     /locus_tag="ACGI68_001076"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFH0137524.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(8966..12535)
                     /locus_tag="ACGI68_001077"
     CDS             complement(8966..12535)
                     /locus_tag="ACGI68_001077"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFH0137525.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(12692..13639)
                     /gene="whiA"
                     /locus_tag="ACGI68_001078"
     CDS             complement(12692..13639)
                     /gene="whiA"
                     /locus_tag="ACGI68_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFH0137526.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(13656..14081)
                     /locus_tag="ACGI68_001079"
     CDS             complement(13656..14081)
                     /locus_tag="ACGI68_001079"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0137527.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(14150..15256)
                     /gene="yvcK"
                     /locus_tag="ACGI68_001080"
     CDS             complement(14150..15256)
                     /gene="yvcK"
                     /locus_tag="ACGI68_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFH0137528.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(15268..16125)
                     /gene="rapZ"
                     /locus_tag="ACGI68_001081"
     CDS             complement(15268..16125)
                     /gene="rapZ"
                     /locus_tag="ACGI68_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFH0137529.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(16156..16869)
                     /locus_tag="ACGI68_001082"
     CDS             complement(16156..16869)
                     /locus_tag="ACGI68_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFH0137530.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(16873..17787)
                     /gene="murB"
                     /locus_tag="ACGI68_001083"
     CDS             complement(16873..17787)
                     /gene="murB"
                     /locus_tag="ACGI68_001083"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFH0137531.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(18060..18830)
                     /locus_tag="ACGI68_001084"
     CDS             complement(18060..18830)
                     /locus_tag="ACGI68_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFH0137532.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            19148..20629
                     /gene="cls"
                     /locus_tag="ACGI68_001085"
     CDS             19148..20629
                     /gene="cls"
                     /locus_tag="ACGI68_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFH0137533.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            20947..21444
                     /locus_tag="ACGI68_001086"
     CDS             20947..21444
                     /locus_tag="ACGI68_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HFH0137534.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            21461..23347
                     /locus_tag="ACGI68_001087"
     CDS             21461..23347
                     /locus_tag="ACGI68_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HFH0137535.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            23370..25151
                     /locus_tag="ACGI68_001088"
     CDS             23370..25151
                     /locus_tag="ACGI68_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HFH0137536.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(25291..27087)
                     /locus_tag="ACGI68_001089"
     CDS             complement(25291..27087)
                     /locus_tag="ACGI68_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HFH0137537.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGLAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENIILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(27299..28228)
                     /gene="hprK"
                     /locus_tag="ACGI68_001090"
     CDS             complement(27299..28228)
                     /gene="hprK"
                     /locus_tag="ACGI68_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFH0137538.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(28228..30045)
                     /gene="uvrC"
                     /locus_tag="ACGI68_001091"
     CDS             complement(28228..30045)
                     /gene="uvrC"
                     /locus_tag="ACGI68_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFH0137539.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(30304..33129)
                     /gene="uvrA"
                     /locus_tag="ACGI68_001092"
     CDS             complement(30304..33129)
                     /gene="uvrA"
                     /locus_tag="ACGI68_001092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0137540.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(33148..35118)
                     /gene="uvrB"
                     /locus_tag="ACGI68_001093"
     CDS             complement(33148..35118)
                     /gene="uvrB"
                     /locus_tag="ACGI68_001093"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFH0137541.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(35305..36567)
                     /locus_tag="ACGI68_001094"
     CDS             complement(35305..36567)
                     /locus_tag="ACGI68_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFH0137542.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(36630..37934)
                     /locus_tag="ACGI68_001095"
     CDS             complement(36630..37934)
                     /locus_tag="ACGI68_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0137543.1"
                     /translation="MKLKRKLATGLAITLLLCSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(37965..38825)
                     /locus_tag="ACGI68_001096"
     CDS             complement(37965..38825)
                     /locus_tag="ACGI68_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0137544.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(38869..39738)
                     /locus_tag="ACGI68_001097"
     CDS             complement(38869..39738)
                     /locus_tag="ACGI68_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0137545.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(39722..40792)
                     /locus_tag="ACGI68_001098"
     CDS             complement(39722..40792)
                     /locus_tag="ACGI68_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137546.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        12986 a         7238 c         5094 g        15917 t
ORIGIN      
        1 catatatttt tactttataa attagcaata ttgcagtttt gctcttaaga aacatctcta
       61 tcatacataa gcatatactc tattatgtat tagtataaat atgccttttc ttatcttaca
      121 taagacctct ttataaagga gcctatcgta acaaatatgt atacattatc acatgtatat
      181 atcatcatat aaaaaatcaa actttattaa aaaaattaat aaattgcttt aaataatacc
      241 acaaaccaaa tttgccatca aaatcacatt aaagtaacaa attgatggca aatttaatat
      301 attaatagta tatagactca tagaatgaac aaaaaaacta cacttatctt ctaatcttct
      361 tatttatttt tgagatattt ttaaatttct tttctttttt tgctaagtat tcgcttttat
      421 tgtctgcaaa tactgcttca gttttaagcc tacgatagga ttcccatctt ttaggcgaaa
      481 gttcttgatg agcaatagct tcccagatag cacagcctgg ttctgaggtg tgagtacaat
      541 tacgaaaatg acattttccg aaataagctt ctatatctgc aaaactttta tctaaacctt
      601 ctgaaacatc ccacattccc aactctctca tacctggtgt gtcaataaca ataccaccac
      661 ctgaaattaa aaatagctct cgtctagtcg tggcatgtct tcctttatca tcatctatgc
      721 gaatttcctt tgtatttaaa aattctccac ccatcagaca attaattaat gaagattttc
      781 caactcctga agaaccaagt aaggcaacag tacagcctgg tttaatatac ttagaaatct
      841 gtttataacc atctggctct atagatgagg taacaactac atctacaccc aaggctatag
      901 aattaactac ttgtaattta cgatgcacat tgtcacataa gtctgattta gtaagtacta
      961 ctactgggat agctccactt tcccatgtta aagacagata cctctccaat ctatgaatgt
     1021 taaaatcctt attcagtgac atgcacaaaa aaacaatgtc aatattagct gcaaccacct
     1081 gctcctcatt tacctttcca gcagccttac gcacaaacaa gctttttcta tgaagcacat
     1141 gatgaataat agcattacca tttttattat tgcaacgatc caacataacg aaatcaccaa
     1201 ctgtgggaaa atcagattgt atagtagcct caaaacgcag ctttcctgaa acctctgcaa
     1261 acagtacacc atttacacat acagcttgat aaaagttctt ttcctgcgag ataatccgag
     1321 caaggtataa atcatgatat tccatacttt gagtaataaa atttctattt agaccatagt
     1381 cttgtaaatt gatttgtttc ataccattca ttctgtttcc tccttttcca acttttcaat
     1441 gattctttct agtgggaaag ggggctgtgc cagtggtttt aatgcaattg acattaattt
     1501 cactgaatta tcatcagctg caatacgatt agaactaaga tgtccacagc gtgtacaacg
     1561 atgaataatt gcccactcac cattttttct cacccacact ccaataggtt ccatattagc
     1621 atcacaatct gcagcacggt caccaggaat gatatctaca tgaagactat gtaaacaatg
     1681 tggacaatga tttctatgtt gtgtaccagc tccttcagga actacaaggc aaccacaatt
     1741 tttgcaaata aagttatcca tacaggaatt ttcctgataa taagctcgtt ttctattttt
     1801 ataattcatt tttatttcct ccgtatcgtg tattttctac aaaaaaagtc cacaggtatt
     1861 tatatctgcc tttttatgtg gtatacatat ttacctacac aaaaacagaa aacccctgtg
     1921 gactagaaaa tttagtatct tctatttcaa cataattata ttcaaaataa tactgtgtaa
     1981 aattaataaa agtgtagcag attaaagccc actacaccac aatttaacaa agtaatatgt
     2041 aatattatta tttgttgtct aatgttagtt atctataaag gcagagtaag acacaacaat
     2101 caaatgtatc aaaaatacac ttaaaaattc tgattaaaca gaacccatat gcgtctccaa
     2161 ctatttattt gttggttacc attatatata actctgacat caggcaagac actccttcac
     2221 taataattat tattgaaatt ttatcataaa atataagtat gttcaagtat aatttaatat
     2281 attcatatat ttatgtttgt ctcattttct tattctataa aatctagtgg tattttgaaa
     2341 tttaatgttc acctagtatt aataataaaa attaactgct attatttgtt ttctcatttt
     2401 atgcctgttg ctcaatttat ctcacttaaa ttaataacaa ctgtcttttc atacaaaacc
     2461 tacaatcaag tttttccata tattctacat gcatagtcta ttttttttga tataaaataa
     2521 cgttcatatt tgtaaattta tttaattttc aaataagtat attgaataat aagcaattct
     2581 attgtattat tgtaattgta gaataaaact aaatcggcaa aactagagaa atttagtgac
     2641 gcaaagctat agggactaac gcttataaaa taaactatga gttatgtcag ccagttgcca
     2701 aaaagagata ttctttttgt ttttatgaaa gttttttagg gggataataa tatatatgtt
     2761 tagagataaa atggatagat gtacacatat gttgactgct tatattggta gttcatatga
     2821 ttattgtgat tttatagata cacaactaga tgattttata ctagagtacg gagaaaatgt
     2881 ggtagaatct tgtttgcatc aagtgatggt attagtaagt aagtataatt aaatagattt
     2941 ggttttctag agaacccttt ttagttttat ctactttaaa tagccttatg ttagaaagct
     3001 aattaagata gatttctagt attcataatt ttatttttta tttaaaatac atataataaa
     3061 gatgtaaatt tgtatcaaat aataaatatg agaacttaaa ttctcttatc tatgtgaaaa
     3121 taaacatctc ttaaaatgaa attctgtaaa tataaatgat tttttagttt gaaggattag
     3181 attaacaaaa ttataaattt gcaaataagc tatattaaaa aatataataa ttttttatat
     3241 aacttattct tttataactt ttaattttaa aagaggagaa tgtatataag agtattcaat
     3301 gtactagaaa aaatcaaaag aaatattaga agaaatggat attgagtaat caaagaaaag
     3361 attttgatat tacaaatgat atgtttcaag aaagcattcc tatcacaaca aaaattattc
     3421 ttgaagacat gccttcaaat gatgaattaa atcatgtttt ttcaaaaggt tgtgaacgaa
     3481 aaatgaaaaa gagaaaaatt gtattaatca cactactatt aataggtgtt ttgttattag
     3541 gaagtatcct atataatctt tttttaggaa aaacagctaa tatttcaatg ctaaaggaat
     3601 cttggaattt tgatatacca atacctaaca aagaaattga agtatttgat actcaagata
     3661 gtattaatgg agatggacaa agttatttta ttcaaggatt ttctgaaaaa aatttcaaaa
     3721 aagtttttaa tttaaaaggt ggtatagttg taagcaaaga taatattaat gaaatagaaa
     3781 aatatattga taaatttaaa agagattctg ttaacattaa taaaagtaat aaaaataaaa
     3841 ttgaagaaga ttttaaaaaa tataagttag aagttaaaaa agatgataag tatatatata
     3901 agagaaatta tgagaactac gtagttttaa ttataaaaaa agatgaacaa aaattataca
     3961 gtttaatatg gaatcagtag gtacatatgt attattaaga attataaata ataaaaataa
     4021 attttagtca atatacaaaa accttttaaa ttgtctggaa atcacctctt catttgttat
     4081 aaaaacatac aacaaatgaa gaggtgattt tatggtttta aaaaaaataa ttataagtgg
     4141 atatgtgctt tcacatgttt atcaacattt gtaatgccta tatttgctaa tgaattataa
     4201 aaatattaaa taatattcaa ctatagatta tgtaaattac tctatgaaaa aaatgagttg
     4261 tataaaatct ggctactgta gattgactat ttcttttaaa attacaatta taataagttt
     4321 tatagaatta aaaataaaac tttagagatt tttgataaca ttgtagacta aatttgccac
     4381 cgaaatcgct ttacagtggc aaatcggtgg caaatacaat atattattag tatataatct
     4441 atataaacat tactattgtt tgattatctt agctacagtt tccacatgtg tagaatgagg
     4501 gaacatatct acaggttgtg cctcaacaca tttaaatccg tgaacattta gataatctaa
     4561 atcccttgct aaagtagaag gattacaaga aacatagaca attctttccg gattcattga
     4621 aacaatagtc tctaatacct tttcgtcaca tccctttcta ggtgggtcta caacgactac
     4681 attggcagtc ttaccctctc tatacatctt aggtacaatt tcctctgcct ttccaacata
     4741 aaattcagca ttatgtatat tgtttaattt agcatttatc ttagcatctt ttatggcatc
     4801 atctataatt tctattccat aaaccttttt agccttttgt gccaaaaata aagatattgt
     4861 acctatccca cagtaaatat caaaaacagt atcagtatca tttaaatttg catattcaag
     4921 agccttatca tacaatactt tagtttggac agggtttact tggaaaaatg ataatggtga
     4981 aatctcaaac actaagtctc ctatataatc gtttattttt ccatctccat atataactat
     5041 attttctctt cctagaatag cattcgtatc tttttgattt acatttacaa ccaaagtctt
     5101 aaatccaggt atattttcct taagcactga agctaattct ttaagatatg gaagtttgtt
     5161 tccatttgct actaatacaa ccattacttc tttagttgta aaacctattt ttgttactaa
     5221 atgcctaatc aaccccgtgt gagtcttttc atcatatatg cttaccttat aggcccttat
     5281 atatgttttt attattttaa caattttatc atttatctca tgttgtatta cgcatttttc
     5341 tgtcgatatt acatcatgag tcttcttttt atagaagcca ataattggaa taccattatt
     5401 cttttgtatt ggaaattgtg ctttatttct atatctatat ggattatcca ttcctatagt
     5461 gtcatgaact tctacatcct ctaacttacc tattctcgaa agtacttgtt taacttcatt
     5521 tgttttcatt tcaagttgtt tattataatc taagtcttgt atctgacacc ctccacaatc
     5581 cctaagattg tcactacaca ctcttttcac cctgaatgga gacttttcta ttatctcaac
     5641 aatatctccc acagcataat ttttctttac tttattaatc ctaactttaa ctttatcatg
     5701 tattaatccg ccctctacaa aaactgtaat accattgtac tttcctactc ctactccgcc
     5761 ttgaccaatg tctactatat ctacaatata catctcgttt ttcgaaagca taaatcaccc
     5821 tccaatcaat ctattatatt taaatcttta caaacttttt tctttaaatt tatattaaaa
     5881 tattgctatt aattaaacta tattttttac taagctacaa tattttattt taccaaaaaa
     5941 aaaggagctt ataaagcccc ttagtaaata aatttattat ttttcttata aaactctagt
     6001 tgaacctcta tataccacgc ctcttgatgc atccacagtt acaacttctc catcttttac
     6061 tagatttgtt atatcagctg cagacacaat aactggtata tctaaattta tacctacaat
     6121 agctgtatgt gatgtcattc caccattctc agttactaca gctcctgctt tttctatata
     6181 attgttcatt tcagcatcag tagcagtaga aacaagtatg tcgccttcat taaagtctac
     6241 acaagcattt ccattcttta ttatacgaac tttaccttct actgtttgag tacctacacc
     6301 tataccttgc acgatttctt cgcttattac atgaaccttt attaaattag tagttccact
     6361 tactcctact ggtactccag cagttataac tactaattcc ccattatcta tataatcagc
     6421 ttgtctagct gcctcaattg ccttttctat aacttcatct gtatttcctg caacagaaga
     6481 cttaactgga tatactcccc atgtaagagc taatttattc atagtctttt cattatttgt
     6541 agtagctatt attggagatt ttggtctgaa tttagatacc attctagcag tatatcctga
     6601 agaagtagat gttattatag ctgatgcatt taaatctact gctgttgtac aagttgcatg
     6661 gcttattgca tctgttacag taacattgtt agtaccattt tcctttagta atctatcata
     6721 atctaaagtc tcttctgttc tttttgctat tgtagccatc atttttactg cctctacagg
     6781 atattttcca gcagcagttt ctccagataa cattatcgca tctgttccat catatatagc
     6841 attggcaacg tctgtaactt ctgctcttgt tggtcttgga tttcttatca tagaatcaag
     6901 catttgagtg gcagtaacaa ctggctttgc taattcatta cattttttta tcatcatttt
     6961 ttgaactatt ggcatctctt cagttggtat ctcaacacct aagtcacctc tagccaccat
     7021 tataccatca gaaactttta atatttcatc taggttctct acaccttctt ggttttctat
     7081 tttagatatt atttgtatat cagttgcatt attgttctct aatacttctc ttattgctag
     7141 tacatcagat gcctttctta caaaagaagc agctatataa tctattcctt gacttattcc
     7201 aaactctata tcacttatat ctttaggagt tattgctggt aaatttatct taacaccagg
     7261 tacatttact cctttatgat ttttaactat tcctgaattt tcaacaacac aaactatatc
     7321 ttctccattt atttctttta ctcttaagcc aactaaacca tcatctatta gtatagaatc
     7381 tcctgattta acgtcttcag caagaccttt atagcttact gtacacattt ctttagtccc
     7441 cataatatct ttcatagtta tagtgaactt ttgcccttct tctaaaaata cctctgggtc
     7501 ttcaaagtta cctgttctaa tttctggacc tttagtatct aataatatag caactggctt
     7561 atttaatttt tctctaactt tctttgccat atcaattctt tctttatgtt cttcatgcga
     7621 accgtgagaa aagttaaatc tacacacatt tagaccattt tgcataagtt gagttaaaac
     7681 ttcctcactt tgtgatgctg gacctagtgt acaaacaatt tttgtcttct ttacattatt
     7741 taacatattt gctcctcctg agatattatt ccacagattt atttttaata ctaaaactta
     7801 tatagataat atttttgcca tctcataagc ttctttatca aatactttct tttgagctaa
     7861 agcttcactt atttctaaat caattatttt gttttctttt attcctacaa ctctagctga
     7921 ttttccatct aataataatt ctactgctct tactccaagt ctacttgcta gtattctatc
     7981 agatacagtt gggcttcctc ctctttggac atgccccaat acagtgactc ttaaatctgc
     8041 tcctgactct tttttaagtt ccttttctaa atcactagca cttccgactc cttctgctaa
     8101 aacgattata ctatgtctct tacctctttt ttgagtagtc tttaatctta gagcaacttc
     8161 atctactgta atttctactt ctggtactat tatagtttcc gctcctcctg caagacctgc
     8221 atataaggct aaatctccac agtgtcttcc cataacttct actatgttaa ctctttcatg
     8281 agatgatgat gtatctctta tcttacctat agcatctata atagtattca ttgcagtatc
     8341 aaatcctata gtataatctg tatatgctaa atcgttatct atagttcctg gtatgcctat
     8401 agctgggaat cctaagtcac taagtttttg ggctcctgca aatgaaccat ctccaccaat
     8461 tacaactaag caatcaattt tatacttttt aagaattttt actgctttta atctaccttc
     8521 ttctgtttta aattcttcac atctggaaga ttttaatata gttcctcccc tgtgtataat
     8581 atctccaaca gaagataagt tcatttcagt taaatcttct tctagtaatc ccttatatcc
     8641 tctattgatt ccatatactt tgcatccata atatattgct gatcttacaa ccgctctaat
     8701 cgcagcattc attcctggag catctcctcc acttgttagt aatcctatag ttttcatttt
     8761 ttacctcctt ggattttatg tcaatttttt attataatca ttgttattgt attctaatgg
     8821 actttttttg tccctatatc actttttaat cccatcaaga ctaaaaaaat taaatgtcct
     8881 taaatgtcca ttttgtgata ttatatcaca aaacggacaa tatttttcat attattataa
     8941 tatatttaga tttttatata aaaaatcaag cttttacttt tatgttatct ttaggaagta
     9001 actcttctaa gttatttata aattcatcac atatacaagt caatgccccg gtcattttat
     9061 atgctttacg tttaccattt atattttcat ccatagggta taagtatata ctgtcattac
     9121 ctggatattg tttggctatt tctacaatat tattagttgt atcagtatca gacatactat
     9181 ctattttcaa atacaatttt ttaccacttt ctggcttaat attttctaca tttctagttc
     9241 ttcctgaatt gtagtttcta tctgcaaagt cagtatcatc atttatatct tttatctctc
     9301 tagctataag ttttgcattt tcgccttctt ttatacttaa tgttcctttt acatatatta
     9361 tattatcttc attcaaaatg acattatact tttgtaataa ttgaggaaaa actattattt
     9421 ctatagcacc atacaaatct tctaattcaa taaatgccat aatttcattt cgttttgttg
     9481 ttttaatagt tttagttaca atcattccac ccattataac atctgtttca ttcatttgta
     9541 ggtatgactc ttcttcctct tttaatgaat ttaattttcc attatccata gatgtttttt
     9601 cttgtagttc tttttcaaat tgtgataaag gatgtccact tacatacatt cccaagactt
     9661 ctttttcaag gttaagtctc tctctttctt cgtactcttg ccttttaggc aaactgtata
     9721 tttgttgaaa atcactatct tcctcactat ctccaaaagc atcaaataaa gaaatttgac
     9781 cttgaacatt tttctttcta tccatagata cactttctaa taaactttca taaccagcca
     9841 tcagactagc tctattttca cttatattat caaaagctcc acattttatt agactttcta
     9901 ttactctctt atttgtatct ttttggtcta atcttttgac taaatctact aaatctacaa
     9961 ataatccatt agattctctt tcagcgatta tattgtttat tatatttacg ccaacatttt
    10021 ttacggctgc tagaccaaat cttatattgt cgccttcaac tgaaaacttt gaaaaacttt
    10081 tgtttatatc tggaggtaat acatctattt ctagtgcttt acattctctt atatactcaa
    10141 ctaccttatc agtatttccc attacactgg ttatcaatgc tgccataaat tcaactggat
    10201 aatatgtttt tagataagct gtctcatatg ctaaaacccc atatgcagct gcatgtgatt
    10261 tattaaatgc atatctagcg aagtctatca tatcatcaaa tattttatta gctatttcct
    10321 cttctacacc atttctgata caccctgcaa tttctatatt accatcttca tcaaatttac
    10381 catgaataaa atattgtctt tcttcttcca tcacgtccat ctttttctta ctcatggctc
    10441 ttctaactag gtcacttcgt ccgtaactat aaccacctaa atctctaacg acctgcataa
    10501 cctgctcttg atataccaaa caaccatatg taacttccat tataggcttt aatttttcat
    10561 gtagatatgt tacatctttt gggttgcttt tattttttat atatgcaggt atagaatcca
    10621 ttggacctgg tctaaatagg gatattcccg ctacaatatc ttcaaaattg tctggtttta
    10681 gctgtttcat aaaacttctc atgccagcac tctccaattg aaacacccct agtgtatttc
    10741 ctgatgaaag catttcatat actttcgggt cgtcataatc catctttgaa aagtctattt
    10801 tttctgtgta attttttata actctatttt tttctattaa atcaagagta tctcttataa
    10861 ctgtaagagt tcttagccca agaaaatcca tctttaaaag cccaagttct tcaagtgttg
    10921 tcatagtaaa ctgtgttgtg attgcatctt gatgtttgta caaaggtaca tattcatcta
    10981 cgggattttt tgatataaca actcctgctg catgagtaga agcatgtctt agcattccct
    11041 ctatctgttt tgatatattt attatttctt ttgtttccgg gtcttggtca tacaattctt
    11101 taagattagg atttgtatct agggccttat ctattgtcat tcctaaagca aatggtattt
    11161 ccttggctac cttgtcaact ttattgtagc ctatatccaa tacccttcca acatctctta
    11221 ttgcagcttt tgcacccatt gttccaaaag ttattatctg ggctacatgg tcatcaccat
    11281 attttctctt tacataatct ataacttctt ctcttctctc ataacaaaaa tctatatcta
    11341 tatctggcat ggatatacgt tctggattta aaaaacgctc aaataataga gagtacttta
    11401 ttgggtctat atcagttatt ttaagtgtat atgctactat cgaaccagcc gcactacctc
    11461 tacctggtcc tactataata ttattttctt tagaaaaatt tataaaatcc caaacaatta
    11521 aaaaatactc tacataaccc attttttcaa ttacatctaa ttcataattt aatctctcta
    11581 gtatctcatt actaggatta tcatatctct cttttaatcc attaaaacac aactccctta
    11641 aataagtttc tggtgtatat ccttctggaa catcatactt aggaagatgt atagtattaa
    11701 aatcaaactc cacattacac atatcagcta ttttcacagt attgtcaata gcctctaatg
    11761 catatggaaa taattcttcc atttcttctc ttgattttaa ataaaactca tcactaccaa
    11821 atctcattct atttgggtca tttaatgttt ttcccatttg aatacacata agtacatcat
    11881 gtattttcga atcttcttta tttacatagt gaacatcatt agtagcaact agtggtatac
    11941 cagtctcttt tgataatttt actaaaccag agtttacttc tttttgctca ggtaaattgt
    12001 gatcttgaat ctccaaaaag aaattttctt ctccaaatat gtctctgtag tcaagagcaa
    12061 attgttttgc tttttcataa tttctattca ttaaagcacg tgctacatct cctgcaaggc
    12121 atgcagaaag tgctataatc cctttgttat gttttttaag ttcttccatg tcaactctag
    12181 gcttatagta aaaaccttct acgtatgaag ttgaaactat ttttattaaa tttttatatc
    12241 cttccatatt cttagctaac agaactaaat gtccttgata tttgtcatag tttgggtctt
    12301 tatctctcaa tccacgtgca gctgtataga cttcacaccc tataattggc tttattcctt
    12361 cttttatagc tgttttgtag aaatctataa caccaaacat acaaccatgg tcagttatag
    12421 ctattgagct catgtttagt tccttagccc tttttataag ttttttcacc ctcgaaaacc
    12481 catcaagtaa actatactca gtatgtacat gaagatgagc aaaatctttg ttcattaaat
    12541 cacctcctgc tttataattt taattttacc acaaatatat gtcctaaaac aaaaacagag
    12601 acttttatct caaaatagga attaacttca acgtatacca ttatatgtta taaattaatg
    12661 cttattctga gattgtcccc tcaatttatt gctatgattt ttttttcaaa tctgttgcaa
    12721 tttcttctat ttttctaagt ctatgattga ctccagattt tcctattggt ggttctaaca
    12781 tctctccaag ttctttgaga gtcatatcct catactcgat tctaatctta gctatctctt
    12841 gtaaattttc tggcaaactg ctaattccta tagtctcttg tatgagctta atattttcta
    12901 cttgtcttac tgctgcattt actgttttag aaaggttagc tgtctcacaa tttactatcc
    12961 tgtttacatt atttctcatt tcttttacta ttttagtatt ttgaagactt aaaagagcgt
    13021 ggtgtcctcc tataatactc aacaaatctg atatttgttc actctctttg atataaacaa
    13081 cgtgattatt ttttctagcc acaattttac tattaaaacc taaagagttt ataagttctt
    13141 ttagagaatc tgcaaactcc tcattattag ttacaaattc catatgatag ttcttttctg
    13201 ggtcgcttat agaacctcca ccaagaaaag ctcctcttat gaaagctctc ttacatcctt
    13261 catgcttaat taagttctct gaaattttgt ttatagtaaa aaatccttct ttatcttcaa
    13321 gtatacctaa cttttttaat aacatttctg aacccatttc gcttgttacc attaatacat
    13381 aactgttatt tctctttagc atttgattct tatttactga gattgtagta ttgatattaa
    13441 aattcttttt taataattta aatactttac gggctattga atttaactct gttgttatct
    13501 ttagatttag cttcttatat ccaacaattt gtatacttcc ccctgattta actagagctg
    13561 acaattcagc tatattacaa cattcattcc cagatataac tcttgctagt tcatttttgg
    13621 tttcagttga aaaagacata tctatcacct attaactaat ttttctttaa cctttttttt
    13681 atttcttgta aggcaacctt tattatctcc ttttcatcat catacctagc taagtctact
    13741 actctatcta ggtggataaa tttagcatca ataaagcctt cctctttttg tggaatagta
    13801 tccatattct ttgcttgcat caaataccaa aaaactgttt tatgaactgc tctattttca
    13861 tcccagtttt ctttgaatgt atagtgtatt tcacctaaat atttcaatat atctgctttt
    13921 actccagttt cttcacttac ttcacgtaat gctgcctctt ggttattttc cccttcttct
    13981 acttttccct tgggtaacac ccaatctcca ttgaacttcc ttagtaaaag aatcgtattg
    14041 ccaaacagga ctacaccacc agcactaacc tcttctctca tcgatgttca ctccttaaaa
    14101 ctaacataat gataaggatt tctacacaat tttttaaaat ccttttttac taacttttgt
    14161 tgtaattatg atttagggct aactcaataa ctatgttaga tatatacttt gcatcatgtc
    14221 ttatataatt attttttatt tcaattaaat tcttctctac agtttttata cccatctctt
    14281 ttaatttatc tttttgcttt ttatctaaca agacttgctc tgctccatct tgtttatata
    14341 agtcaagcat ttcttctggc aatatctcat tatttgcgat tacataatct attaaattct
    14401 catcagtgtg ttttactata gcattaacat gctctaatac attgtaccct tctgtctcac
    14461 ctggttgagt cattatattt gctatatata cttttggagc tacagacgat ttaatagcgt
    14521 ctacaatccc ctctacaagt aaatttggta ttatacttgt atataaactt ccaggaccca
    14581 taattataat gtcagcatca taaatagatg caataacatc ctttaatggt tttacatctt
    14641 ttggatttaa aaaaatttta tcaatagagc ttttttgaat ttttgactct tcaggtatac
    14701 tagattctcc atttattata ttgccatttt ctaatttggc aactaaattt acatcttcca
    14761 aagttactgg taaaactctt cctgttatag caaaaatttc acttaattta tatacagctt
    14821 tttcaaagtt tccatacaac ccattcatag cagccaaaaa caaatttcca aaactctgac
    14881 cttttaaaag cccttctgta aacctatatt gcataacttc attcattgtt ggctctatat
    14941 ttgcaagtgc taataaacag tttcttatgt ctcctggtgg gagcattcct aagtcttctc
    15001 tcaatacacc tgaacctcca ccatcatcag caacagttac aattgcagtt atattttgcg
    15061 tagtgtgttt aagacctctt aaaaataccg attgaccagt acctccacct atcacaacca
    15121 catttggttc tctattttca acaatagacc tttgaaatag ttttactttt tcattttttg
    15181 cttttttata tataaataac gatacaatta atgcaatcca acctaaaatt cctacaataa
    15241 taagtaattt agccatgttt gcctccatta tcgcaacata gagtctctat gtttctttac
    15301 aactctatat cccttattag ataaatcctc cgctataaga tttgttatag taactgacct
    15361 atgtcttcca cctgtgcatc caacaccaat tactagatgt tgttttcctt cctctatata
    15421 ttgaggtact aaaaaatgaa tcatatctaa aagtttcaca taaaattctt cactgatttt
    15481 agaattcata acataatctc taacttcttt atcatcacca gttttagctc ttaactcttc
    15541 aacataatag ggatttggaa gaaatcttac atcaaatact aaatctgcat cagcaagtat
    15601 accatgttta aatccaaaag atactacaga aatagttagg tttggattat cttcaccaga
    15661 agaatatatt ttctttattt cttccttaag gtctttaggt ttcatgttag ttgtatcaat
    15721 tatacatgta gataactcct ttaaaggttc cattatcttt ctttccattt ttataccttc
    15781 tggaatctgc atatcttttg ccaatggatg atttcttctt gtcattttat atctctttag
    15841 caatacatca tcattacaat ccaaatatac catttcatat tcataatttt ctttttctaa
    15901 ataatttaag ctttcatgaa gcgcttcaaa aaactttctc cctcttatat ctatccctag
    15961 agcaacttta tcaatacttg aatttggttg ataacataat tctgcaaatt tagttatcaa
    16021 agcaggaggt agattatcta cacaataaaa tcccatatcc tctaaagctc tcattgtctc
    16081 actttttcca gatcctgaaa gacctgtaac tataacaaat ttcattgcta actcctccct
    16141 tttatcttat gtttattata ttataatgtt ttctactaac gatatatcca gattagagtc
    16201 agatatagtc ttcatagcta gttcaaaatc accaactctt tctggacaat gtgcatctga
    16261 gcctattata aatttatttt ctagcccttt tattttattt aattgattta cattcaaaaa
    16321 agaatggcta ctatttatct caagtctagt atttgtcttt tttgctgtct ttgctacttc
    16381 ttctatatac acatcgcctt tatctcctgg atgagtaatt ataaaaatat cattattact
    16441 tatcgcattt attacagctt ttgtattata ttcttttgct ttttcagttt taaaaatata
    16501 gttattacaa tgatttaata agcttcttaa tgatgttgga gtagaaccaa aatgatatcc
    16561 agccataata taatcaaaat ctctaataaa tttatcatcc atatctatat ttcctttatc
    16621 atctaatatg ttacactcca tacctaaaag tatatttata tcagtgtact tatcatttaa
    16681 ataatctact tcttctctca ttttgagaat atcttttttc tttacaccat atgcaatatg
    16741 tttataacta tggtcagata ttcctattgt ttttattcct ttagaaatgg ctacctttac
    16801 attatcctca atagtccctt tgccatgact atataatgta tgtgtatgat aatcagccag
    16861 tattttcatt tgttaatcct ctccaagtat tttgacttct tcttccaaca taaccccaaa
    16921 cttggcatat acagcacttt ttataacata tattaaatcc aaaatatctt tagcacttgc
    16981 ttctccttga ttgactacaa atccacaatg cttctcagat acttgtgctc ctcttaaagt
    17041 aagacctctc aatccagttt cttctattaa tttacctgca aaatatcctg ttggtctctt
    17101 aaatgtactt cctgcactag caaaatttaa tggctgttta gttattcttc tcgtcgctaa
    17161 atcatccatt aaacttttta tttcatcata atttccttct tgtaattcta attctgctga
    17221 taaagctata tatccgtttt tcgatattat actttttcta tacccaaatt ccatctgttc
    17281 atttgataat tcaaatatat tgccttccat atccatcagt cttactgact tcactatatc
    17341 cttcatttca ccaccatatg ctccagcatt catggctaat gctcctccca atgtgccagg
    17401 tattcctgat gcaaactcaa acccttttaa ttcttgcttt tggagagctt tccctaaaac
    17461 tgataataaa gctcctgact gagcagtcat cctagtatca tttatctcat aagaattgaa
    17521 attatcagct aattctatta ccactcctct tattccacca tctttaatta aaatgtttga
    17581 accatttcca attattaaat acggaataga ctcttttttt accaacttaa gtacattttt
    17641 taattgttct tcagttctag gtctaactaa tatatctgct ggtcctccta ccctaaatga
    17701 aatatgtttt ttcataggtt catcgacttt tatatcctct ttacttaata tatttaataa
    17761 atttatatat atatcttgat tattcattgt atttcctccc aaattatact gaactttagc
    17821 tgtcattatt atttaaatta cattaatata tagtattaac tatattcaat aatatatgat
    17881 tttgcttatc atatggattt tatattttaa gcttcttaat tacaaactat attcaataaa
    17941 tatataataa agtaaaaaaa aaaaaataac aacatataaa agaatataaa taatttaaaa
    18001 aaagcatcaa tatcactgat atcaatgctt taaaaggcaa tttaattcgt attatttatc
    18061 taactatttt tgacatagca aagataataa atccctccaa ctactatgga acctccaacg
    18121 atatttccaa gtgttacaaa taccaaatta gttataaatt gaaccatagt tatatttgct
    18181 cctaatgcca ttcccattgg tataaaaaac atgtttgcaa cactatgttg gaatccacat
    18241 aatacaaata gcattattac tgtccaacat gaagccaatt ttgacattat atcttgagat
    18301 gctgtagcca tccatacagc taaaacgact agaatattac aaagtattgc ttttaaaaac
    18361 attgatgcta tagttgaatt aaccttagct tctgccacag ctatagcttt tgtagctgca
    18421 tctcctgtca atgtagaaga ataaaacaca agtgcaacta gcaaaattga acctactaaa
    18481 tttgcaaaat aaaccagtcc ccagtttctt aacatttttc ctaatgttat ttttttgtcc
    18541 ataagtgcaa tagccattaa agtgtttcca gtgaataatt ctgctcctgc cactactaca
    18601 agcataagac ctactggaaa gaccatagca cttaaaaagc tagctacccc tgggtctatc
    18661 ttgccaaaag tttgactaac tatcatactc cccaatgccc caaaacctat aaatgctcct
    18721 ccaaataaac ctaatagaat tgcattttgt gtcgaaatat ttgctttttt aatgcctgca
    18781 cttatagttg cttgtgcagt ttccccaggt gtcaagaatc ctttactcat aaatccttcc
    18841 cccttaaaaa ttatactttc ccttgtcttt ttatggcaat aattatataa taacaatatg
    18901 atatataata aattttatta acataaaaaa tatactatat acttatatat acttgtctca
    18961 tataattata ctatttatta tataacaact ttgttaattt cgaaataatt tatattgaat
    19021 ttgaaaaatt taccttttga ttatttattt tactttttaa ttattgaggt attatatata
    19081 atagatatat gtttgtgtta ttatatataa ttacttattt taataaatat ttgaaggagg
    19141 gaattttatg tttgatatat catttttcga aatgtcaata tatgaaatag cagttactac
    19201 aatatatata attaatttca tggttatact aaaccttatc tttagagaaa aaagaaacat
    19261 aaatacaact ttaacatggc ttcttatatt agtattgatt cctgctcttg gattcatact
    19321 ttatatagct tttggcagaa atatttcaaa aaacaatatg tttagattga aagaaaagga
    19381 tgataagata atcaaaagta atatactaga tactcaggtt aaactccaat ctacatctga
    19441 aattgattca gatatacatc aacataagga tatgatttat gcattggcta attcaaataa
    19501 tgctcattat accaataata acgatgtgtg gatttatgct gaatctagtc aattttttaa
    19561 cagtcttctt gaagaactta aaaaagctaa aaaatatata aacatacaat tttacatttt
    19621 caaagatgac aaaatcggaa ctgaaataat agacattctt atagacaaag ctaaagaagg
    19681 tgttgaagta agacttttat ttgatgcagt tggtggtaga acactaaaaa atagtacact
    19741 ttccagactt aaagaaagtg gtgttaaagt tggaagcttc ttcccatcat tcttgaaaat
    19801 tgtaaacttt aacttaaact atagaaatca ccgaaaaatt gttgtcattg atggtaaggt
    19861 aggatttgta ggtggatata atgttggtga tgaatattta ggtagaaatc caaaatttgg
    19921 tctatggaga gatacacata ctaagcttac tggtgattgc gtaattgacc taaatatgag
    19981 attcatcctt gattggagat atacaactaa agaagactta gatttagaaa aatactttat
    20041 agaagaagat acaaattcta atcatccttc tgaaaatatt gggattcaga tagtatcaag
    20101 tggtccagat atatcagaac ttgatgagat taaatatggt taccttaaaa tgattcagaa
    20161 agctagaaag tatatataca tacagagtcc ttatttaata cttgactcaa cttttataga
    20221 tactttaaaa atagcctgtt tatctggtgt tgatgtgaga gtgatgattc cttctaaacc
    20281 tgaccatccc tttgtatact gggcatcata ttcatatgct ggagaacttt taaagtttgg
    20341 agctaaaatc tatacttatg gtcaaaatgc atttttacat gctaagacaa tagttataga
    20401 tgactctata tgctctatag gaacagctaa tatggatatc agaagttttg agcttaattt
    20461 cgaagttaat gcatttatgt actcttcaaa aaaagcattg gaacaaagaa ttatatttga
    20521 aaatgatatt ttaaattcta aggaaataac attagatata tataattcta ggtctactta
    20581 tataaaaatc aaggaatcaa tttcaagact attatcagca gttttataaa tcttttattt
    20641 tataaaaatt ttacattttg gtttattttt taaagttttt tatatgtatt attttttttt
    20701 tattgaattt agctcatttt ttttattttt tttaactatt ctgaattatt ttatattata
    20761 atagactagt atagttttta tttttaatag gtaacttttt tgcattgttt ttataaataa
    20821 agaaattgtt gtatattatt ctttctttat tgttttggtt ttgttcaccc acataatgtt
    20881 ggggtcaact catggttaat tattttatga aaaataataa ctttaaaaag aaaaggggga
    20941 tttgttatgt gcgattttat ttcacacaac aagcaattat ttgacgaact agatatattt
    21001 attgactctc ttgcaacaaa ggaaggtgcg ttaatacaag ttctacatga agcccaaggt
    21061 atatttggat atcttccaaa agaagttcaa cttcatgttg ctagaaaact tggagtagct
    21121 cctgcaaagg tatatggggt tgttacattc tattcttatt ttactacaga acctgtaggt
    21181 aagtataaaa tcagtgtctg tctaggaact gtatgttttg taaaaggtgc tgacaaaatc
    21241 ttaagtgctt ttgaaaaaca gcttggcata aaagttggag aaactactag tgattttaaa
    21301 ttttctttag aaggtttaag atgtttgggg gcatgtggtt tagctccagt tgttactgta
    21361 aatggtaaag tttatggtaa agtgaaacct gaccaagtaa gcgaaatatt agatacttat
    21421 agagaattag aattgaattg ttaagagggg tgaataaata atgcgtaaag taaattcatt
    21481 tgatgaatta aaagttttag ctgatgagct gaaacctaat cttagcttaa gaaaaaatta
    21541 ttctaaggaa aatgtattaa gaagagaatt acttgtttgt tgtgatactg gatgtacatc
    21601 ttcaaacagt ctagaaatag ttagtgaatt agaaaatgaa ataaagaaat caggaatcca
    21661 agataaagtt agtgttcgtt tgacaggttg ttttggtttc tgtgctcaag gtcctattgt
    21721 aaaagtttat cctgataacg ttttctacgt aaaagttgag ccatctgatg ctgaaaagat
    21781 agttcaaagc cacttaatta gaaatacagt tgtcgaggaa ttattatatg aggaagaatc
    21841 tttaagtaaa aaagttgaag aacaagaaga aatgtctttc tacaaaaaac agttacgtat
    21901 agctttaaaa aattgtggtc ttatagaccc agatagtata gaagaatata ttgccaatga
    21961 tggttattta gctctaggca aatgtttaac ttctcttact cctcaagaag ttattgcaga
    22021 ggtaaaaact tctggccttc gtggtagagg tggtgcaggt ttccctactg gctctaaatg
    22081 ggaagctgct tcaaaaaatc cagctggtcc aacagataaa aagtttgttg tatgtaatgc
    22141 tgatgaaggt gacccaggtg catttatgga tagatctgta cttgaaggtg accctcatag
    22201 tgtactagaa gctatggcaa tatgtggata cgctataggt tctgatacag gatacatata
    22261 cataagagca gaatatccaa aatctataga aagattaaaa gtagccatag ctcaagctga
    22321 aaaatacggt ttacttggcg aaaatatctt aggcactgga tttaacttta aattagaatt
    22381 aaaatatggt gcaggtgctt tcgtttgtgg tgaaggtaca gcgctaatgc attcaattga
    22441 aggtagacgt ggtgaaccta gaatgaaaac ttatagttct tctaagagtg gtctatggaa
    22501 atctcctact tgcttaaata acgttgagac ttttgctaac atacctgcaa ttatacttaa
    22561 gggtggagat tggtttgcca acttaggtac tgaagattct agtggtacta aagtctttgc
    22621 tcttggtgga aaggtagaaa atgtaggact tgttgaagtt ccaatgggta ctactttaag
    22681 agatattgta tttgaaattg gtgggggaat ccctgaaggt aagaaattta aggctgttca
    22741 aacaggtgga ccatctggtg gatgtatacc aactgaacat ttagacactc ctatagactt
    22801 tggttcatta agttctatcg gttcaatgat gggctctggt ggtatgcttg ttcttgatga
    22861 atctgactgt atggtagata tagccaagtt cttccttgag tttactgttg aagaatcttg
    22921 tggtaaatgt acaccttgtc gtataggtac tacaagatta ttagaaatat taactaaaat
    22981 tacagaaggt aatggtactc tacaagattt agatgaccta gaaaaccttg ctgaaactat
    23041 acaaactgct tcattatgtg gtcttggtaa agctgctcca aaccctgttt taagtactct
    23101 tcaatatttc aaagatgagt atatagctca tgttgtagat aagaaatgtc ctgctggtaa
    23161 atgtcagaac ttactttctt actttataac agatgactgt aagggttgta caaaatgttc
    23221 tagagtgtgt cctgctggtt gtataacagg ttctgttaag gaacaacata caatagatac
    23281 ttctaagtgt ctaaaatgtg gtgcttgtat agataattgt acatttaacg ctataatcaa
    23341 gaaataactt atttggagga ggaataacaa tgagtttagt taatttaact attaatggca
    23401 aacacgtttc agcacctagt ggaacttcta tcttagatgc tgcgaaatta ataaatataa
    23461 aaatacacaa cctttgtcat ttacatatga atgaaataga taagcttgac acttgtgctt
    23521 cttgtcgtgt ttgtatggtt gagacagaga gaggattagt tccagcttgt ggaacagtta
    23581 taaaggaagg tatgaaagtt cagactaata gtgctaaagc attaaatgct cgtagaacta
    23641 ttgttgaatt attgttatca gaccatccac aagattgttt tgtctgtgaa aaaaatggag
    23701 attgtgaatt acagactata gctgctgatt taggagtaag aaaaataaga tatcaaggtt
    23761 ctaaatcttt tgcaggtaaa gatacttcta ctaaatcttt agttaaagac cattctaagt
    23821 gtatattatg tagaagatgt gagactgtat gtaatgacat acaaacagta ggagcattat
    23881 ctggagttaa tcgtggattt aatactttag ttagtacatt ctttaactct gatatggttg
    23941 aaacagagtg tacattctgt ggtcaatgta tatctgtctg tccaactgga gcattaacag
    24001 aagtagataa tgtacctaaa ctttgggatg ttttaaataa aaaagaaaag actatagtag
    24061 tgcaagttgc tcctgctgta agagttgcta ttggtgaaga gtttggatta gaaccagggt
    24121 caatctctac agggaaaatg gtagctgctt taaaagcact tggatttgaa catgtatttg
    24181 atacaaactt tggtgcagat tttactataa tggaagaagc tactgaattt atagaaagaa
    24241 tccaaaaagg tgaaaatctt cctatattaa caagttgttg tcctgcatgg gtaaacttct
    24301 tagagcacaa ttatccagac aagttaaact tagcatctag ctgtaagtct cctcaaggta
    24361 tgtttggttc tatagctaaa aactactatg cacctaaaat attaggaatc aatcctgaag
    24421 aactttatgt tgtatctgta atgccttgtg tagctaaaaa atatgaagct tcaagagaag
    24481 aattaagtga atctggaata ctagatgttg acctttctat aactactaga gagttagcta
    24541 agatgataaa agaagctgca atcgatttac ctaatcttca agaccaagat tttgataatc
    24601 ctctaggaaa atctacaggt gctgcatcta tatttggagc aagtggtgga gttttagaag
    24661 ctgccctacg tacttcatat gagaagataa ctaataagac tttagataat gtaaacttca
    24721 ctaatgttag aggtcttaaa ggaattaggg aagcaagtat agatgttgat ggaactacag
    24781 ttaatgtatg tattgtaaat acacttaaaa acgctagaaa gattatggat aaggtcagaa
    24841 gtggagaatg taaataccat atcatagaag ttatggcatg tcctggtgga tgtgttggag
    24901 gtgctggtca accttatcac catggtaata ctgaaatagt tgatagacgt gcaaatgcgt
    24961 tatatgaaat agatagaaat aaggctattc gtaaatctca tgagaatcct gatttacaag
    25021 ctatttataa agacttcttt ggagaaccaa atagtgatgt agctcataaa taccttcata
    25081 ctcattactt tgataagagt tgtgtctatg gagagtgtcc acaagaatgt gcttgtgaag
    25141 aagctaagta gtcataacaa agtataaaat ttattcttaa aaaaggattg tcttaaaata
    25201 cttttacgta atttaagaca atccttatta atttcacaaa ttaaattata tttctaaata
    25261 gacttattta ttaattaacc tatctattaa ttaaagaata cctgccattc tcataagttg
    25321 ttttgcattt gtagttttac atcttccatc ttttatctta tagtcaaaat gaattttatc
    25381 gtctacatag tactctgaga aatgatagtt tacaacttca acatctccgc tacctgaaag
    25441 gtcacatagc tcaaagtcat gtgttgtcac catacttata acccagggtt ttgataattt
    25501 ctttactgcc tcacttgctc caataattct atcagcagag ttagttccct taaaaatttc
    25561 atctactaga accaacattg gcagttcttt taaactatat tgaatcattg attttattct
    25621 aagaatctca gcatagaaag ttgatattcc ttgacttaca tcatcttgta ttctcataga
    25681 agtaaatata gccatacagg tcgcatcaaa actcttagcg catactggag cacctgcata
    25741 tgccagcact aagtttacac cgatacttct taaaaatgtt gtctttccag acatatttga
    25801 acctgtgata atacaagtct gagaatttaa ttgtatcgaa tttgctatga cacttttttc
    25861 tgctattaat gggtggtata cctcttcaac tttcaatctt ggagtatctt catattttat
    25921 tactggaaaa catgtatttt cctttacatg agaaacaact gttaaactta ttagagcttc
    25981 aaattctcca atagattcaa accaatctct aatttgattt ccatatatac ctttccatcg
    26041 ctccaaagct tctgcacaat gatagttcca catcaaaaca ccacaggcaa ttatatatag
    26101 tatctgatta taccttaggt ttacagcatt tcctattgaa tttaattgtt taacacctct
    26161 tgaaactcca cttcccttag aaagtttatc ttgtaattct ttcaaatatg cactctcaaa
    26221 cgtctctttt tctaaaacct caaacatttt ttcatatact tgtatacttc tactaaatga
    26281 aaataatgga gcaagagttt gcataagtct tggatatcca aaaccactaa accctaactg
    26341 gattataaaa agtaaaaaaa caggtaaaac aggtaaaaat cctaacatac aaaatataaa
    26401 agataagatt gttgcagttg gtaaacccca agtaaaaata tgcatccatt ttggtatata
    26461 cacattctta ttttctccat attctataaa cgattctata ctactatctg aattgttttt
    26521 ttgctcttta ccaattatat tactgagtgt ttgaatatga atagaaaaat catcttttga
    26581 taaaagttct tttatagctc tctgtctttc taaaattata ttctcatctg gattttcatt
    26641 ccacaaatat ttggctaaaa aatttttccc ataagatgta ttagctacac agatatattg
    26701 atacaatgat gctcttccaa ataagtctaa atcttttgct tgcgaatttt catcttttaa
    26761 gtattcttta ccatctattt taaattcctt ccaaccatct ccaaatctct taatatactt
    26821 atttaatact tcgctcttac ttttaaaata tgtttcttct tcttttactt tactatgaat
    26881 aactaagagt gctataaata ttattattga taaaactcca acaaaaatac ctactatatt
    26941 tttattataa aatccaatag caagccctgc tatcactatc aaaaaagata ttaatcttgc
    27001 tgtacttatg ttatttgact ttttacttaa gagcaattct ttttcagtac tactttctaa
    27061 aagtttacta aaatattcta ttcccataaa aaattccaac tttcttgtta tataatgaaa
    27121 ctcaaatata aaattaatct agtattatta taatctaaca ctgtacttta aattatcctt
    27181 atataatata tcttattaat aaacattata tatacaactt gattttaatt tataaggtgt
    27241 aggacataat cctaatcaat atttaattaa atgatgtgta gtccactatt attcagtact
    27301 acttagtatc gattaatttt gataattttt tagtaaattc ttgagctgca tcgtatccca
    27361 ttcctctttg tctaaaattc attgctgcta cttctaatat catagcagta tttcttccag
    27421 gtttaacagg tacaactaat ttctctacat taacaccaag tatttcttca tattctttat
    27481 ccagtcctag tctatcataa tacttagttt catcccaagc ttctaaatga actactaaat
    27541 ctatcatttt agaatttttg attgccccta ctccatataa acttctaaca tctattattc
    27601 ctattcctct tatctcaaga aaatgtctta ttagttcagg tgactgaccc acaagcatac
    27661 tttcatctaa ttttcttatt tctacagcat catctgcaac taatctatta cccctttgaa
    27721 tcagttctaa tgctgtttcc gactttccta tactactttc accttttatc agtacaccta
    27781 taccataaat atctactagc acaccgtgaa ttgttgtatg tggtgctaac ttattatcca
    27841 aatagtttga cagtctattt ataagtctag tggtatttct atttgtgctc aaaagtattc
    27901 ttttatattt tttagccttt tcaattacat ctggcttgat ttccaagtct ctagaaatta
    27961 ttaacgctgg tatttcatat gagaaaaatc tatctagtac ttcttcccga atatgactat
    28021 ccattaatcc aaaaaaatta tattctgtct ttccaactat ttgtattctt tcatatgcaa
    28081 aataatcaaa atatcctgca aactgtaatc ctggtctatt tatatcttga gagtagacat
    28141 aataatctac atcatcaggc atgtatacta cttttaactt caactctttt attatctctc
    28201 ttattgatac tttattgcta gtttccatta attttcttcc tttctaaaaa aattataaac
    28261 attatctgca atgcttttat tcattccatc tacttctaaa agttcttcca tagtagcttt
    28321 ttttatagca tctacatctt taaagtgatt taataatgat ttctttcttt tttcacctat
    28381 tccttgtata tcatctaaga tagattttgt caaagcttta tttcttagac ttctatgata
    28441 tgttattgca taattatgaa cttcttcttg tatactagca ataaatctat ataaattagt
    28501 tgttttatct agttctattt ctttttcttt gcatatcaat cctttagttc tatgcttatc
    28561 atctttgtac attccccaaa gaggtatatc tacatcattg agttctagta ctttttttac
    28621 tgcactaact tgtccttttc caccatccaa caatatcaag tcaggtaaat ttccatgttt
    28681 taatcttcta tctacaattt cagccataga atcataatca ttaggtccaa taactgtctt
    28741 tattttatat ctcctatatt ccttcttatc ttttttagca ttcgtataca ctaccataga
    28801 accaatcgaa tctacacctt gtatatttga tatatcataa gcctctattc ttattggcaa
    28861 cttttctaga tttaatattt gctttaattc ttccaatgct ccaatacttt tttcatattt
    28921 tcttttattc atatcagaaa atttttcaag atattctaca gcatttttcc taaccatttc
    28981 aactaagctt ttcttctctc ccttttgagg aactcttatt gttacttttt gccccttttt
    29041 agatgacagc cattcttcta atatagaact atcttctatc tcatcttcta taattaattc
    29101 tttaggtata tactcttgtt cattataaaa ttgttttaca aatgaactta atatagaagc
    29161 tcttggacta tccattaccc cttctaatat aaaatgttcc ctacctacaa tcttaccatt
    29221 tcttataaaa aatacctgga cacaagcttc attatgagct cttgccattg caaccacatc
    29281 ttggtttaag tcacttacag tagcatctat tttttgtttc tgtatcatct cttcaaggct
    29341 ctttatctta tccctatata cagcagcctc ttcaaatcta aagttcatag aactctcatt
    29401 cattttttcc tttaaaagtt ccatcaattt ttcttcttta ccagataaac acattataat
    29461 ctcttctatc atttttccat actcttcttt agaaacattt cctgtacaag gtcctaaaca
    29521 tttatttatg tgtagattta aacaaggtct tgtcttattc tttattgctt tatctatatc
    29581 tattttacag cttcttattg gatacgtact acttataagc tccaaggtat cattcacagc
    29641 agttatattg gtatagggcc caaaatactt tgccttatct ttaagaactc ttctaacttt
    29701 taagattctt ggataatctt cattcgtagt tacttttata tatggataag ttttatcatc
    29761 tctaagcact acattatact ttggtctgta tctttttatt aaattacatt caagtattaa
    29821 tgcctctaac tctgaatccg ttataatata ctcaaactta tatatattct taaccataga
    29881 ttttacttta gatgtatggt tttttgaaga ttgaaaatac tgccttaccc tattttttag
    29941 agatatggct tttcccacat atattatatg gtcgtatttg tctttcatta aatatactcc
    30001 gggctcagaa ggtaattttt ttaaatgctc ttgtatatca aacatctcgc cctccaaaac
    30061 attaaatttt atactaaaat tataccctaa aatatgaatt ttaaaaagtt atacaatttc
    30121 cagtgcttta aacaagttaa agcccatatt cccttctata attgatttga aaacaatgtt
    30181 aaccatgaag agtaatatgg actttacttt gtattactat ttacaactta tatttgacta
    30241 atttacttat aacaaaacag tttatcgtat atctgctcta aaatttttca caatttatat
    30301 aacttaagaa aaatatttct ttaagaattg acctgtataa gaaccatcaa ctttagatac
    30361 ttcttttggt gttccagttg caactatagt tccaccctta tctccgcctt ctggtcctaa
    30421 gtctattaca taatcacaag tctttataac atctaaatta tgttctatga ctataattgt
    30481 atttcctgta tctgctagtc tttgtaatac ttggattagc ttgtctacat ctgccatatg
    30541 aagtccagta gtaggttcat ctaatatata caaagtcttt cctgttggtc ttttactaag
    30601 ttcagcagct aattttatac gttgtgcttc tcctcctgat aattgagttg aaggctgtcc
    30661 taatttaata tatgaaagtc ctacatccat taaagtttct aattttcttt ttatatttgg
    30721 tatattttcg aaaaactcaa cagcttcttc cacattcata tctaatatat cagatatagt
    30781 tttatctttg tattttactt gcaatgtttc tctattgtat ctttcacctt tacaaatttc
    30841 acaaggaaca tatacatctg gtaagaaatg catttctatt tttattattc catcaccctt
    30901 acaagcttca catctaccac cttttacatt aaaactaaat ctaccttttt tataacctct
    30961 tgctttagct tcattagtag atgcaaaaat atctcttata aaatcaaata cacctgtata
    31021 agtagcagga tttgaacgag gtgttcttcc aataggactt tggtctatat ttataacttt
    31081 atctatactt tcaattcctt ttatctcctt atgcttacca gctctttgct taagtctatt
    31141 cactttactt gcaacacctt tgtaaagtat atcgtttatt aatgtacttt ttccagaacc
    31201 tgaaacacct gttatgcatg taaacttacc taatgggaac tttacatcaa tatttttcag
    31261 attattttct gatgctttaa ctatttctat aaaacttcca tttccttctc tagtagtctc
    31321 aggaagcaat atttgttttt taccacttaa atattgaccc gttattgagt tctcattttc
    31381 aattatttca tctaaagtac cctgtgcaac tatttcacca ccatgtatac ctgcacctgg
    31441 accaatgtct ataacataat cagcttctct cattgtgtct tcatcatgct ctacaacgat
    31501 taaggtattt cctaaatctg ttagatgtct aagagtagtt ataagcctat cattatctct
    31561 ttggtgaagc cctatacttg gctcatcaag tacatataaa actccaacca atgcagaacc
    31621 tatttgagta gctaatctta tacgttgcgc ttctcctcct gataaagttc cagcttttct
    31681 tgataaattt aaataatcaa gacctacatc tcttaagaac atagctcttc cttttatctc
    31741 ttttataatc tcatgagcta taaatctctg tttttctgta agatttatat tttccataaa
    31801 gtcaatcaat tcatttacag ataagtttgt aacttccatt atattttttc catcaactaa
    31861 aacagataaa acttcctttt taagtctcat ccctttacac tttggacatg gtctctctgc
    31921 catgtattct tcaatcttat ctctcatgta atctgaatta gtttctctat atcttctctc
    31981 taagtttact attacacctt caaagtatga tttataattt ctagttcctc caaattttga
    32041 ctcataagta aactctacaa ttatattatc tttaccataa agtaattcct ccacaaaatc
    32101 atctggtaaa tctttaaacg gtgttgtcaa agatacatta aaatgctctg ctaaactttt
    32161 tatcatttta ctgtaataag tgtcatcatt aacacctgtt gaaccccatg ctgccactgc
    32221 accttgtttt attgataaat ctttgtttgg tactactaat tctgggtcaa cttctttact
    32281 ttccccaaga ccattacaaa catcacaagc accaaaggga gcattaaatg aaaacattct
    32341 tggagataat tcttctatgc caattccatg ttctgggcat gcaaatttag ttgaaaaaag
    32401 aatttcttct ccatctatta tttgggctat tacaagacca tcagatagct taactgcagt
    32461 ttcaattgaa tctgctagtc ttgattctat tccatcttta attactattc tgtccactac
    32521 aacttctata ttatgttttt gatttttatt taagattata tcatcactaa cttcatagtt
    32581 ttcaccatta actctaactc ttacaaatcc ttcttttttg atattatcaa agactttttt
    32641 atgtgtacct ttttgtcctc taacaattgg tgaaagaatt tgtaatttcg ttctatctgg
    32701 aaaatctagc attttatcaa ctatttcttg aatagtcatc tgacttattt tttcaccaca
    32761 tgtagggcaa tacacatctc ctactcttgc aaataatagt ctcaaataat catatatttc
    32821 tgttactgtt ccaaccgtag aacgtgggtt ttttgaggta gtcttctggt caattgagat
    32881 agctggtgat aaaccctcta tatattctac atttggcttt tccatctgac ctaaaaattg
    32941 tcttgcataa gcagatagac tttctacata tcttctctgt ccttctgcat atattgtatc
    33001 aaatgccaaa gaagacttcc cagaccctga caaacctgta aaaacaataa atttatctct
    33061 tggtaattct aaatctatat tttttaaatt atgttctttt gctcctctta ttataatttt
    33121 atcttccata atgttgtttt tacctttcta tttttccaat ttttcttcta attgtttcac
    33181 tttatctcta agttctgctg ctctttcaaa ttgtaagttt tgagcagctt ccatcatctc
    33241 tgattttaat ttatctatat tagctcttat ttcgtcttta tcatctgtat cagatattcc
    33301 atatacaact tcttcctctg caaccttagt agcttctata ctatctctta tatttttctg
    33361 tatagtttta ggaatgatat tgtgctcttc attatataaa ttttgtatat ctcttcttct
    33421 ctttgtttca tcaatagcat tttgcattga gtcagttatc ctgtctgcat acataattac
    33481 tcttccattt tcatttctag ctgcacgtcc aatagtctgt attaaagctg tttcggaacg
    33541 taagaaacct tctttgtctg catctaaaat tgctataagt gaaacttctg gtatatccaa
    33601 tccttctcta agtaagttta ttcctacaag gacatcaaac ttccctaatc ttaaatctct
    33661 tattatttct gttctttcta atgtaactat atctgagtgt aaatatttaa cttttatccc
    33721 tatttcttta agatagttag tcaagtcctc actcattttt ttagttaaag tggttataag
    33781 cactctttca tttttttcta tagttttagt tatttctcct accaaatcat ctatttgatt
    33841 atttataggt cttacttcta caattgggtc taaaaggcca gttggtctta ttatctgttc
    33901 tgctattgtc tctgagtgtt gaatttcata aggccctggt gtagcactta caaacaacat
    33961 ttgatttata tttccttcaa attcttgaaa atttaatggt ctattgtcaa gtgcagaagg
    34021 taatctaaaa ccattctcta taagtgatgt ttttctagac ctgtctcctg catacatacc
    34081 tcttacttgt ggtatagtga catgtgcctc atctacaatt atcaaataat catctgggaa
    34141 gaaatccatt aaagtatatg gtctttctcc ttctgacctt cctgtaatat gtctagaata
    34201 gttctctatc ccctgacaaa atcctatttc ctttaacatt tctatatcat acttagttct
    34261 ttgttcaatt ctttgtgctt ctaatagcct atcattttcc ttgaaaaatt taatttgttc
    34321 ttgaagctcg ttttctattt ctacaattgc cttttcaatt ctttctggcg ttgttacata
    34381 gtgagatgct gggaatataa caacatgatt tctagtacca acaatcttcc ctgttacata
    34441 atcaatttct gttattctat ctacctcatc accaaaaaat tctattctta ttgctttttc
    34501 atcattacta gctgggaata tttctagtat atctcctcta actctaaagg tacctcgtgt
    34561 aaagtttatg tcatttcttt cgtattgtat ctctattaac ctttttatta catcatctct
    34621 gtctctttgc atacctggtc ttatagataa cataagttct ttatagtctt ttgggtcacc
    34681 aagtccatat atacaagata cagatgatat tattatagta tctcttctct ctaaaataga
    34741 tgctgtagca gaatgtcgaa gtttatctat ttcatcattt atgcttgcat ctttttctat
    34801 ataagtgtca ctgtgagcta catatgcttc tggttgataa taatcatagt aacttacaaa
    34861 gtattcaact gcattgtctg ggaaaaattc tttaaactca ctgtaaagtt gtgctgctag
    34921 agttttatta tgagccatta ttagggtagg tttcttaact tgttgaatga tatttgccat
    34981 tgtgaatgtt tttcctgaac ccgttacacc taataatgtc gaaaatttct catttctatt
    35041 tatagaatcc actattgatt taatagcttc tggttggtca cctgtaggtt tgaagtctga
    35101 ttttatttta aaatccatag tatcactcct tttttcgaac attagttcct gacatgtatt
    35161 attatatcat acctttataa aattgtttaa gttattttaa atattttacc catttaaatt
    35221 ttacttaaat ctaaaacata aaaaaagact tctatctttg caatagaagt ctataattca
    35281 tggaaaataa atactttatt caacttacat aataaaatca aatgcttctt tttcaactcc
    35341 atctaatttt aaattaaaca tttttaatat agttgctgca tgtgctctca ggtctccttc
    35401 ttctataacc acaccacctt taatatttgg tccaaaagct ataaatggag gtctattacc
    35461 tttagtaggt aaatgaccat gtgtcgcaac agcaaacttg taatctttta tatctgaatc
    35521 ttttataata cttccttcga aatcatttcc aaatgaaatt ccttcaagac cttcaattac
    35581 catttcaaaa tcaccttcaa gaccaaggtt agatgcttcc tctttggtaa aaatattctc
    35641 tatgaataat tcttctttaa attcttcaag cagttctctt aactctgtta acctatctaa
    35701 gttatttact attatttgag ctgatattcc agaagaattt acataggcat caaaatcctt
    35761 aacttctcca ttttcaactt ttataaatcc atttgattta agcaagacat ttggatttat
    35821 aactttatca actctaagat gaccatggtc cccaagtatt ataaaatttg tatctttgta
    35881 tgtacctgca tcctttgtag cctgaataat atctcctagc catttatcat tcatttttaa
    35941 tgctttatct acttcgtcat taaataagcc ataattgtgt cgtgtatggt ccaatgtagc
    36001 taaatgtatt agcattaaat ctggtttata ttcctttatt atatctactg aacagtttac
    36061 tccaaataaa tccatgttgg gttctaattt ccaatcgata taatgacaat gtctattgta
    36121 aataccgtcc attataacct tactggaact tcgttcaaaa gtttctcttg ggtcatcttc
    36181 tctactagga gcccatattt ctgcaatatt ataatctata tttggatttg ctcccattac
    36241 aggccataat acactagagg tagttagatt attttctttt gctacatcta taatagtttt
    36301 tgcttttatg tcctttgagt accagtacca gtctttattt tctttatttg ggtcaaattt
    36361 ttcattatga tatataccat gttttttagg atatactcct gtaaccatac tagtatgaca
    36421 cggatatgtc agagtgggat aaacacaatt tatatttttt actatggagc aattttctag
    36481 tatagattta aagtttggta gattttttac aaattccaag tctttttcaa ataaagaatc
    36541 tactgataat actataacct ttgtcattta cattacctct tcatttatat attattgaat
    36601 acttttaatc aacttacaag tctatataat tatttatttg ttttatctat tactgcttgc
    36661 attgcagaag ttcctttgct tactacatct tttggatttg catttaaatc agtaagcccc
    36721 tttttaatct catccattag cttatcagtt gcttccttgt atccatttaa cattggaagt
    36781 gcaacagcat attgtaattg gtcagttgca accttatatt gaggtttttc tttatataat
    36841 gtttgtaact tttctgagtt tgttgcatct tcagttgttg gtaagtatcc tgttgatgaa
    36901 ctaaactgaa cgattttatc tgtttgtgtc atccattcta taaattctgc acttgcttct
    36961 ttttctttat ctgttccacc ttcaagcata actagattag ctccacctgt aggagttgcg
    37021 tatttttgat tttttggtaa gaatgctgtt gatatttcaa acttaccttc tgtttgttgc
    37081 ataagtcctg ctaaatctgc tgttgatgta aatatcatac ctactttacc atttacaaaa
    37141 tcttgttttg ctacgtccca ggcattataa tcttctccag gaggcatttt cattattcca
    37201 gctttcatca tatcttgcca gaacttaact ggtgcttgtc ctgcctcatt attaaatgct
    37261 acttttttac cattagttat ttctcctcca ctttgcatta ccatagcttc atagaaccaa
    37321 atatctattg gtgtttcaaa accataagta tcttctgatt tatttgtcat tttactagca
    37381 taatccttaa gctcttccca tgattttgga cctgaagggt caagacctgc tttagttgcc
    37441 atatctttat ttatgtataa tattggtgta cttctattaa atggcatacc aacaaactta
    37501 tcctcataat atgaatttgt taaaagacca gaaaagaatc tttcttgata tgttttatca
    37561 aaatatttac tcatatctac taaagcacct gctgatgcga atgtacctgt ttgagttatt
    37621 tctaataatg ttacatctgg ttggtcttta gatgttaagg ctgcctgtag ttttgttgca
    37681 ttttcatagt aatctttctg ttttaacata tttacttgta catccttatg ttcactatta
    37741 aattggtcaa ccattttttg agcagtttct ccattagttc cacctaaagg agcccagaaa
    37801 tctatcttcg taatattgct atcagtcttt tctcctttag atgcatttgc atcattagaa
    37861 tttgaacacc caactaaaga acataataat aatgttattg ctaatcctgt tgctaatttt
    37921 cttttaagct tcattttcat cctccaaatt tagtctttaa ttttttattt tattcctgaa
    37981 tacataaaag cttcttttac ttttttattc gcaaatatat aaacaagaat tattggtaaa
    38041 attagaatta tatttcctgc cattattaca ttccatgcag taattccttc tgaagattta
    38101 aggagcgcaa ctcctattgg taaagttctt attgcatctg tatttgtcat tactaagggc
    38161 cagaaataat tattccaatg gtatataaaa ttaaataata taaatgttat tactgctggt
    38221 tttgccatag gaaccattat tctaaacatt atttttaagt cagaagcatt atctagttta
    38281 gctgcttcaa taatttcatc tgaaatctgc ataaaattct gtctaagcag aaatatccca
    38341 aatgaagatg tcataaatgg aattataagt ggaacataag tatttataaa tccaaactta
    38401 ctcatcatca tatagactgg tagaaaagtt acttgtggtg gaatcataag tcctacaagc
    38461 aaaagcccaa atagcatttt ttcaccttta aatttaatct tagaaaatgc atatgctgct
    38521 ggaaccataa ctaaaaattg tacaattaaa acacttcctg ttacaaatac actgtttagc
    38581 aaatatctaa aaaatggacc tgaatgccat gctgtactat aattttctaa cactatatca
    38641 tttggtataa tagtgggcgg taattgcatc atttcttgtg gatttttaaa ggatgtagat
    38701 agcatccata taaaagggaa tataaatatt gcagataaaa gtaatagaat taatatttct
    38761 attgattttg atgctatttt ttttatatca actttgtctt ttttgatagg ttgtataata
    38821 tccattgtct cctccttttg gataaacctt ataatatcta ctttttcttt attgataatg
    38881 aactttcttt gataagagtc taaagttagc atatgtagca atcataacta tagctaacaa
    38941 aaacatactc gctgcactcg cttgccctat gtcatagtat ataaatccag actcatatat
    39001 ataataagat aacatgttag ttgaacccac tggcccacct tgtgtcatta tacttatctc
    39061 atcaaatact tgaaaagatg cagttgttgt tgttatcata agaaaaaata ttgttggtga
    39121 taatagtggc aatgtcactt taaagaaatt cgttattttg tttgcattat ctaatttaga
    39181 tgcttcatat atatagtttg gtatggattt aagacctgat aacactatta agatattata
    39241 tccaagagtc ttccatattc ctattagaat tagtgacatt aaagctgaac tttcacttga
    39301 aagccacttt gatgttggta aatgtaatgt cgatagtatc cagtttaaaa agccatattg
    39361 tggctccata atccaaagcc acaatacagc tacagatact agtgaaatta catgtggtgt
    39421 aaagattata ctttgcgtta aattgtgtat tatagaattt ttatttagcc aaacagcaag
    39481 tactatggct agtgatatag atatagtcac cataaaaaaa gtataaattg cagtgtttgt
    39541 tatagtttgt ataaactctg gattctttaa aagttctgta taattgttta agcctacatt
    39601 ttcaaaggtt gaactaagaa gactaccaga tttgaagctt agagatatcg tcttaattat
    39661 tggatagact ataaacaata aagttcctat aatagcaggt gttatatata aatatgcttt
    39721 attaaattgc tttttcataa atatctcctt tgattttaga atacatactt ttacatactt
    39781 ttacatcttc tattctttct ccatttttat caaaataata taaatctttt tctaatacac
    39841 ttattcttaa agttcttcca atatcaaata tatcattttc cgttttgact cttatctctc
    39901 tattaagaac agataaacaa tatatagttt catttcccaa cacttcttta gttacaactt
    39961 ttccaaatag actaaataca tcatcatttt tcccatctat gtttatactc tctagtgttg
    40021 ctttttctgg tctgaatcca aatttaatat cattttttat atctagtatg ttcatacaag
    40081 gactccctat aaattgagct acaaataaat ttttaggatt ttgatatatc ttctttggag
    40141 aacctatttg cattatcttt cctttgttca ttataactat cttatctgcc attgacattg
    40201 cctcaacctg gtcatgagtt acataaacaa atgtagactt tagtttttga tgtaattgta
    40261 ttaattctgt tctcatttga tttcttaatt tagcatctaa atttgacaat ggttcatcca
    40321 ttaaaaatac ctttggattt ttaaccatag ctctagcaag tgccactctt tgtctttgac
    40381 ctccagataa gttgcctggt ttcacattta aatattctct caatcctact acatcaataa
    40441 cttctgttat aagtttttct cttttttctt tttctacttt tttgttttta agaccaaatt
    40501 ctatgttttc tctaacagtc atatgtggat ataatgcata attttgaaat accatagcta
    40561 tatccctttt actagcatcc gtttttgtaa catcattttc ttcaatcttc aacctacctg
    40621 aagttatatc ttcaagacct gcaatcattc ttaacgtagt agatttacca catccacttg
    40681 ggcctaccaa tactgtaaaa gaaccatctt ctatagtaag atttaaattt tctataactg
    40741 tatttttttt attataagat tttgttacat tttctagtct tatatctgcc atttttatcc
    40801 tcccaaaata tttaatttaa ttctacgatt tacttataga ttaataatac tcttaatttg
    40861 taaaggatat attaagacaa agtgaatata tggtaaactt attaaaataa aggttaaatt
    40921 ataaacattt tttgttaaat atactgaagt taagatgata tacagcttat gttagctata
    40981 attttctata tttttcatat tcatggtttg ctatcacaat gtaattttaa tttatgaaga
    41041 gtcatataaa actatttatt ttttatataa tttggtttga aagtaaaaaa gacctctatg
    41101 ggggatagag gtctttaagt gggggagatt gagtaattta tttttgctat attaagtttt
    41161 acaattatat tatgtcccaa atttacaagg tttaaacata aaaaataaaa attttttaat
    41221 ttttataaat ttacc
//
LOCUS       DAYASV010000020        40842 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYASV010000020 DAYASV010000000
VERSION     DAYASV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.020"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            207..866
                     /gene="cprR"
                     /locus_tag="ACGI68_001099"
     CDS             207..866
                     /gene="cprR"
                     /locus_tag="ACGI68_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HFH0137547.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGSDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1077..2135
                     /locus_tag="ACGI68_001100"
     CDS             1077..2135
                     /locus_tag="ACGI68_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFH0137548.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPEKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2386..4530
                     /locus_tag="ACGI68_001101"
     CDS             2386..4530
                     /locus_tag="ACGI68_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2800..2802,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFH0137549.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4579..5412
                     /gene="fdhD"
                     /locus_tag="ACGI68_001102"
     CDS             4579..5412
                     /gene="fdhD"
                     /locus_tag="ACGI68_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFH0137550.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYVNTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDKNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5423..5596
                     /locus_tag="ACGI68_001103"
     CDS             5423..5596
                     /locus_tag="ACGI68_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137551.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5654..6205
                     /locus_tag="ACGI68_001104"
     CDS             5654..6205
                     /locus_tag="ACGI68_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137552.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6230..7606
                     /locus_tag="ACGI68_001105"
     CDS             6230..7606
                     /locus_tag="ACGI68_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HFH0137553.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7606..8121
                     /locus_tag="ACGI68_001106"
     CDS             7606..8121
                     /locus_tag="ACGI68_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137554.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8199..9482)
                     /locus_tag="ACGI68_001107"
     CDS             complement(8199..9482)
                     /locus_tag="ACGI68_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HFH0137555.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9670..10092
                     /locus_tag="ACGI68_001108"
     CDS             9670..10092
                     /locus_tag="ACGI68_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HFH0137556.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10194..11132
                     /locus_tag="ACGI68_001109"
     CDS             10194..11132
                     /locus_tag="ACGI68_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFH0137557.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11318..13342
                     /gene="ligA"
                     /locus_tag="ACGI68_001110"
     CDS             11318..13342
                     /gene="ligA"
                     /locus_tag="ACGI68_001110"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFH0137558.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13502..14851
                     /gene="rph"
                     /locus_tag="ACGI68_001111"
     CDS             13502..14851
                     /gene="rph"
                     /locus_tag="ACGI68_001111"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFH0137559.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRTIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14851..15321
                     /locus_tag="ACGI68_001112"
     CDS             14851..15321
                     /locus_tag="ACGI68_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0137560.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTNMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15515..16801
                     /gene="tig"
                     /locus_tag="ACGI68_001113"
     CDS             15515..16801
                     /gene="tig"
                     /locus_tag="ACGI68_001113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFH0137561.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16951..17535
                     /gene="clpP"
                     /locus_tag="ACGI68_001114"
     CDS             16951..17535
                     /gene="clpP"
                     /locus_tag="ACGI68_001114"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFH0137562.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17554..18804
                     /gene="clpX"
                     /locus_tag="ACGI68_001115"
     CDS             17554..18804
                     /gene="clpX"
                     /locus_tag="ACGI68_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFH0137563.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18875..19402)
                     /locus_tag="ACGI68_001116"
     CDS             complement(18875..19402)
                     /locus_tag="ACGI68_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0137564.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19390..19902)
                     /locus_tag="ACGI68_001117"
     CDS             complement(19390..19902)
                     /locus_tag="ACGI68_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0137565.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20082..22445
                     /gene="lon"
                     /locus_tag="ACGI68_001118"
     CDS             20082..22445
                     /gene="lon"
                     /locus_tag="ACGI68_001118"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFH0137566.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22435..23046
                     /gene="yihA"
                     /locus_tag="ACGI68_001119"
     CDS             22435..23046
                     /gene="yihA"
                     /locus_tag="ACGI68_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFH0137567.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23311..24681)
                     /locus_tag="ACGI68_001120"
     CDS             complement(23311..24681)
                     /locus_tag="ACGI68_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0137568.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24856..24971)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25259..26338
                     /locus_tag="ACGI68_001121"
     CDS             25259..26338
                     /locus_tag="ACGI68_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137569.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26729..27409
                     /locus_tag="ACGI68_001122"
     CDS             26729..27409
                     /locus_tag="ACGI68_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0137570.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27402..28781
                     /locus_tag="ACGI68_001123"
     CDS             27402..28781
                     /locus_tag="ACGI68_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HFH0137571.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28952..30037
                     /locus_tag="ACGI68_001124"
     CDS             28952..30037
                     /locus_tag="ACGI68_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFH0137572.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKVKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30091..30450
                     /locus_tag="ACGI68_001125"
     CDS             30091..30450
                     /locus_tag="ACGI68_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0137573.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30503..31375
                     /locus_tag="ACGI68_001126"
     CDS             30503..31375
                     /locus_tag="ACGI68_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HFH0137574.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKNIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRGFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYINSIGMII"
     gene            31499..31819
                     /locus_tag="ACGI68_001127"
     CDS             31499..31819
                     /locus_tag="ACGI68_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137575.1"
                     /translation="MKSINELEDYISQNKLVDKSKNTLPTTDKTREKFSKIKLNEIKK
                     IYETIGINSITKISIDKNKLEMEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRF
                     RIKS"
     gene            31834..32418
                     /locus_tag="ACGI68_001128"
     CDS             31834..32418
                     /locus_tag="ACGI68_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137576.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVELNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANVLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32671..33708
                     /locus_tag="ACGI68_001129"
     CDS             32671..33708
                     /locus_tag="ACGI68_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFH0137577.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLNGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33789..34154
                     /locus_tag="ACGI68_001130"
     CDS             33789..34154
                     /locus_tag="ACGI68_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137578.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34159..34476
                     /locus_tag="ACGI68_001131"
     CDS             34159..34476
                     /locus_tag="ACGI68_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137579.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34524..36128
                     /locus_tag="ACGI68_001132"
     CDS             34524..36128
                     /locus_tag="ACGI68_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0137580.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36252..37880
                     /locus_tag="ACGI68_001133"
     CDS             36252..37880
                     /locus_tag="ACGI68_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFH0137581.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38145..39494
                     /locus_tag="ACGI68_001134"
     CDS             38145..39494
                     /locus_tag="ACGI68_001134"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFH0137582.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39650..40729
                     /locus_tag="ACGI68_001135"
     CDS             39650..40729
                     /locus_tag="ACGI68_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFH0137583.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16785 a         4306 c         7351 g        12400 t
ORIGIN      
        1 taaattttta tatttaagta aatacgtgta aaatgaatat aaagttttga aatataaagt
       61 ttttgtatct aagatatttt atttgtaaaa tagtaattaa ttattgtaag ctataatatg
      121 agagtattat ggttaatgtg aattttaaag atattattaa atatataaat tagaatacta
      181 ttaaacggat aaatggagga gttattatgt ctaagatatt agtagtagat gatgagttag
      241 atatgttaaa gttgattgaa aatgtgttga aaagagatgg acatgaagta aaggtaatta
      301 gtgatgtaga aaaagctatg aacatggatt ttaattattt taatctaata atattagatg
      361 ttatgatgcc taagatggat ggttttgaag tatgtaaaac tataagaaat aaggttgatt
      421 gtccaatact gtttttgaca gcaaaaaata tggaaagtga tattatgtac ggtcttggta
      481 taggtgcaga tgactatata accaaacctt ttggtgtagg agaattaagg gctaggataa
      541 ctgctcattt aaggcgagaa agtagagaaa aaaagaattt attaactatc tcaaatgttc
      601 aatttaactt tttaggcaaa gaagtttcaa ttgatggtaa aaaaattaat tttactaaaa
      661 gtgaatatct aatatgtgaa tttctagcaa aaaataaggg acaagtattt tcaaaagaaa
      721 gaatatatga gtcggtatat ggttctgatg gagaaagtga tataagtact atcacagagc
      781 atataaaaaa tatacgaagc aaattaaaac aatatgatat ttatacaata gaaactgtat
      841 gggggatagg gtataaatgg gtgtaataag tataagtgtt gtttagtgaa aatattatat
      901 atatctacaa aatgtttgta aaatattaat ctatatatta aaatttaatt tatagattaa
      961 tattttttgt aaatgacaaa attgtaaggt attttgttat aaaatattaa gattcatagt
     1021 gtatactaat aatatagtat ttagtataaa tcttgagcaa aggatggtta tttctaatgg
     1081 atagactaaa gaatattaga aagagtttac tacagtatac atttttattt ttgttaatag
     1141 gtataacaat ttacctagtg tttaaaacat tggatattaa aatgctatca aatgttatgg
     1201 ttatggtaga taagaagttt ttattcatag gtgctttggc aataatgtgt cacataatgc
     1261 ttgaaggagt agtaatgaag ataattatag aaagcactca caaagttaat attagattta
     1321 ttggttttaa gctagccatt atgggatttt attataattt aataactcct tttgcttcag
     1381 gaagccaacc agtacagata tatgtactta aaaaatgtaa aatgccactt agtaaggcaa
     1441 gtgcagttgt taccaataag tctattatct tccaaatagt agttactttt tattgtacaa
     1501 tacttgtttt aatgaatttt gctatgttag aaaaacaaat gaaaatgatt atgcctctag
     1561 tgtttctagg gatagccatt aattcatttg tcttaattat gataatatta gttattttaa
     1621 atcctgaaaa aataaaatat tttatgagaa ttactataaa gcatttatca aaaattaagt
     1681 ttttaaaatt tttagataat aagattgaat ccatagaaag ttttatagat gattatagta
     1741 aagcaataaa tttttttgca aagaataaaa aggtattaat ttcaactata atagtaactt
     1801 ttattcaatt aagtgcttac tttagtgttt cattttggat atataaagca tttaatttac
     1861 aaggttatag ctatatttat atactgacat tacaagcatt tttatatatg gcaatatctc
     1921 caataccaac acctgggaat gtaggagcta atgaattagc ctttttcact atattcaaat
     1981 cagtattccc tcaacctttg atgggatatg cagtattttt atatggtgga tttatgtact
     2041 atttgattct gattggaagt ggaatattta ctgtaattac tcactataga atgaaaaaca
     2101 gaattggcag aaatattgtt ttaagtgaaa actaaataag tttaaagaat atatattgag
     2161 tatattaaaa aatatgtcat aagaactaga atattttaat taaatggttg aaatataaag
     2221 attattatga catatttttt ttgaaattta agaggaattg tgagaaaatt gttgaattta
     2281 atagatagta ttgcaaaata tttatgattt tgcatattat aaaaacactc attatcttta
     2341 agataaattt tacttatcag gggataaatc taagggggga attgtatgga gaaaaaaatt
     2401 ttaacagttt gtccatactg tggagctgga tgccaattat atctagtagt aaaagataat
     2461 aagatattaa gagctgaacc ggctaatggt agaacaaatc aagaaagttt gtgtttaaaa
     2521 ggtcgttatg gatgggattt tctaaatgac cctaaaattc taacacctag gctaaaaaag
     2581 ccaatgataa gaaaacaagg tgtactagaa gaggttgaat gggatgaggc tataaactat
     2641 acagcttcaa gactaaatga aattaaagaa aaatatggac ctgattctat tatgggaaca
     2701 ggttctgcaa gaggacctgg taatgaagca aactatataa tgcaaaaatt tatgagagct
     2761 acaataggaa ctaataatgt tgaccattgt gctcgtgtct gacatgcacc atctgtagcc
     2821 ggtctggctt actcattagg tagtggtgca atgtctaatt ctataccaga gatagaaaat
     2881 gcagatgttt tatttatatt tggatataat ggagcagatt cacatccaat agtggcaaat
     2941 agaatagtaa aagctaaaaa aaatggtgct aaattaattg ttactgaccc tagagtaact
     3001 gaatctgcaa gaattgctga tatacatcta cctataaaag gtggaacaaa catggtttta
     3061 gtaaatgctt ttggaaatgt attaatagaa gaaggtcttt acaataaaga atttgttcaa
     3121 aatcatacac aaggatttga tgagtataaa gaaattgtta aaccatatac agctaaatat
     3181 gctgaaaaga ttacaggaat cccagaagag cttataagaa aagctatgag agaatatgca
     3241 aaaggtaaaa aagctatgat actttatgga atgggtgtct gtcaatttgg tcaagctgtt
     3301 gatgtagtta agggacttgc atcaattgct ctacttacag gtaattttgg tagagaaagc
     3361 gttggaatag gacctgtacg tggacaaaat aatgttcaag gagcttgtga tatgggagct
     3421 cttcctaatg tatatcctgg ctatcaaaat gtaactgatg ataagataag agaaaagttt
     3481 gaaaaagctt ggggggtaaa attatctcct aataatggtt atagtttaac tcaagttcca
     3541 aatttagtac taaaagagaa aaagcttaaa gcttattata tatttggaga agacccagtg
     3601 caaagtgacc cagatgcatc agaagttaga gaagcattag atgaattgga atttgtcatt
     3661 gttcaagata tatttatgaa taagacagca cttcatgctg atgttatact tccagctact
     3721 tcttggggag aacatgaggg agtatataca tgtgcagata gaggattcca attgatgaga
     3781 aaagctatag agccacaagg agatgtaaaa ccagactggc aaattataag cgaaatttct
     3841 acagcaatgg gatatcctat gaattataaa aatactaagg aaatatggga cgaacttaga
     3901 caattatgtc caagtttctt aggtgctact tatgaaaaga tagaaactca aggatgcgtt
     3961 caatggcctt gtaagagtga aagtatggaa gataaaggaa ctatgtatct gtatgaaggt
     4021 caaaagtttt ctactcctaa tggtaaaggt aatttgttcg ctgctgaatg gagaccccct
     4081 atggaagttg aagatgatga atacccattt agtttatgta ctgtaagaga agttggacac
     4141 tattcagtaa gaacaatgac tggtaattgt agaacactta gttcattaga agatgaacca
     4201 ggacgtgttc aaataaattc taatgatgct gaaaaattag gaattgaaga tgatgaatta
     4261 gttagaatta gttcaagaag aggaagtgta ataacaagag caacagttac tgatagagtt
     4321 aaggaaggtg caacatatat gacttatcag tggtgggttg gagcttgcaa tgaacttaca
     4381 atagcaaatt tagaccctat atctaaaaca ccagagtata aatattgtgc tgttaaactt
     4441 gaaaaacttg aagaccaaga attagcagaa aaatgtgtaa gagaagagta tcaaagtttg
     4501 aaagacaaaa tgacagctac aaatatctaa tgatttatta gatttaagat ttataaaagg
     4561 cagaaaggct aatattttat gaacaatata tactcaaaaa ctaaattaca agaaaatagt
     4621 aaggataaag tttttgaaaa tagttatagt ctagtggact acaaatattt aaattctttt
     4681 aatgtagaag taagcaaaat aaatgaacat gaagtatcaa tagataatga agaaataatt
     4741 gcagaatatc cacttagttt tattttaaat gataaatatg taaatacatt tttatgtacc
     4801 ccatataatt tagaggaact tatagtagga ttcttgaaaa caaaaggata catagaaaac
     4861 aaaaaaagct tattaaaatt tgagataaat gagagtgaac gttttgcaaa agtaactatt
     4921 caagagaaac aaaaaaatca agatgagcaa attatatttt taaattcact agactatata
     4981 acatgtgctc ctgtaaagag caatataaaa ataaattatg atacgattta cagtataatg
     5041 gataaaaatt taaattcatc agaattattt aaaaatactg gtggggtaca tagcgtatct
     5101 gtatttaatt ttgataaaaa aatagtcact tgtgaagatg tagcaaggca taatgcaatg
     5161 gataaggcta ttggattttg tgttttaaat gacataacat taaaagataa aattgtggct
     5221 gtaagtggaa gaatatcatt ggaaatgata ttaaaagcag ctatgactca aattcctatt
     5281 gtagtatcaa aatcagcacc gactaattta tcaatagagc tttctaaaaa attaaatata
     5341 acgcttattg gatttgtaag aggaagacgg atgaatattt atgcaaatcc acagagagtt
     5401 atcaaaaagt gagaatgaat atatgtgtac taaaaaagaa gagcaaagat attcaaagtg
     5461 tgaaatctgt ggagtattaa ttgatgatac tgataaggtg tataataaca catacaattt
     5521 gtgcaaaaaa tgtaaacgaa atataagtac tgagaaaata gtgaaatata taaatatata
     5581 tgatgaaagt aagtagacat atgatgaaaa taactagagg gttggaaata tacaaattga
     5641 aaggtgggat aatatgaaaa ataaaatagg ctcttttgta atagcagacc caaacaaatg
     5701 tacaggttgt agagcttgtg aagtagcttg ttttacaatg cataatcagc ataataatgt
     5761 gggttataca gttggaacag ttgatatacc tgttatacct agactttact tagtaaaagg
     5821 tgacaatttc tgtatgccaa tacagtgtag acattgtgaa gatgctcctt gtttaaactc
     5881 atgtccacaa aaagctatag ttaaagaaaa caatattatg tctgtaaatg aagaaaagtg
     5941 tataggatgt aaaacttgtc tgctagcatg tccttttgga gctatagact tacttccaca
     6001 atatgaagat ggtaaagaag tagaacaagt aattttagat gaaaataaga agatagctta
     6061 taaatgtgac ttatgtaaag acaatgaaaa aatagcttgt ataagtgcat gtcctcagga
     6121 agcattaaaa cttgttactc caatagatga caaaaaagct aagaatagac aagctgcact
     6181 tagtttattg ttaacaaata aataaaggat acaggggtag gtgacgttta tgataataaa
     6241 tatagataag gatttatgta ctggatgtag agaatgttca aaggtctgtc cagtaaatgc
     6301 tattgaaggt gaagagggaa agccacaaga gataaattta gatagatgtg ttatgtgcgg
     6361 tcaatgtgtt caaacttgta aatcttatgc ctcagttata gatgaaggat ttgagttttt
     6421 acaagagaaa aaacaagaaa gagagatacc agagtctata aatgaaccta tatttgcagc
     6481 atacaatgta tgtgatatag ataaggtgaa gaaagcttta agtgacccaa atgtattcac
     6541 tatggtccaa tgtgcaccag cagttagagt tgctttagga gaagattttg gatattattt
     6601 aggatactta ggtgctggga aaatggcagc agcacttaga gctttaaatt ttgatagagt
     6661 ctacgatact aattttgcag cagatttaac tataatggaa gaaggtagtg aattaattaa
     6721 aagagtgaca gaaggtggaa ctcttccaat gtttacatct tgttgtccag cttgggtaaa
     6781 attcatggaa aaaaattatc ctaaattaac taatcattta tcttcttgta aatcacctca
     6841 gcagatgggt ggtgcaatat ttaaaactta tggtgcagag ataaataatg tagatgcatc
     6901 aaagatattt agtgtttcta taatgccatg tacatgtaaa aaatatgaat gtgatagaga
     6961 agaaatggat tctagtggat atagagatgt agatgtagta ttaactacta gagaactagc
     7021 ttatttaata aaagacatgg gaattgattt taaaaattta agagaagaaa aatttgatag
     7081 tcctttaggt agttatagtg gagctggaac tatctttggt gttactggtg gggttatgga
     7141 agctgctata agaacaggat atgagcttat aactggagaa agtattcctg atgtagaagt
     7201 aaaacaagta agaggagaaa atggttttag aaagtcaact attaaaatag gagacttaga
     7261 cttaagagtt ggagtagtaa gtggattaaa aaatgtaata ccagttcttg aagacttgga
     7321 aaaaggaaaa ttagatgtag attttattga agttatgact tgtcctgttg ggtgtgtaag
     7381 tggtggaggt caaccaaaaa ttttactaga agaatataag gaattggctt atgaaaatag
     7441 aacaaaagca acatacgtgc atgatgaaca tttagcaatt agaaaatctc atgagaatcc
     7501 tgatattaaa aaactgtatg atgaatatct ggtagaacca ttaggagaaa aatctcatca
     7561 tctattacac actacatatt gtataggaaa ggaagtggct aagtgatgaa ctattttgtt
     7621 atagcagacc ctgataaatg cataggatgt agaacttgta tgataggatg tgtagttgca
     7681 cattcagatg aagatatttt ttaccaaaat ccagatgaaa taaattttaa tccaaaactt
     7741 tctgtcataa agacaaaaga agtaagtgct ccaatacagt gtagacattg tgaagatgct
     7801 ccttgtgcaa aggcatgtcc aaatggtggt atagttaggg ttggtaacac aataaaaata
     7861 aatgaagaaa attgtatagg atgtaagact tgtatgttag catgtcctat aggtgcaata
     7921 gacatagtta cattaaaaga tgtagatgaa ggaaagcttt gctttagaga aagaatgaca
     7981 gcaaataagt gtgatttttg tatagaatcg ccagaagggc ctgcatgtgt aaatgtttgt
     8041 ccaactaagg catttactat agttaaggat gaggatatag atagtaaagt taaaaataag
     8101 agaaaattag ctatattgta atataaaaaa gatggagttt tatttatgat taaaagctcc
     8161 atctttttta ttataatatt tatatatcta aaaatcaatt attgcattag tttatctaat
     8221 ctttctttat tcaatttttt cattcctata gaagttataa taagtaaagt ataggcaatt
     8281 ataactgata aaattataat agaccaagaa aatctgtatc caaaattatc aactaacaat
     8341 ccaaataatg ttgaacctaa tgcaaatccc actgcaaaaa ttaattgtac gattccatat
     8401 atgctaccaa actccttttg accaaagaaa gagccagtta aataagcagg accaatcata
     8461 taagcaaata cagaaagacc tttcaaaatg gcaaatacaa atgcaaaagt agaagattta
     8521 tctaccaata gtaatgataa agttgctata agaaccaata caaaagatat aataagtggc
     8581 tttgtttgac cgaatttatc aaacaataat cctccaaata aattaccaat gagagcaaat
     8641 aaggcaaatg tggagcctac tgttcctatt agaataggat ttagataatt tctaagataa
     8701 gttggatatt gtacagataa tgatgaaaca tatataccaa caaatataaa tccaatagtc
     8761 ataatccaaa aatatttaat ttttttagct tcttttaatg tataacccca tactgaagga
     8821 gaaattgtat cagatgcttt ctctttttta cctttagatt ttaccttttc attaatatct
     8881 ttaggcatcc tgagtatgaa taatattaca ggtataccaa ctattaatgc tataattcca
     8941 aatattagat aagaacgact gtatccttga gttgctaata agcgaactgc aagtggttgt
     9001 agaaatatac taccaataga accaccagca aaagcaaagc ccaaagcttt tcctttgctt
     9061 tcttcatcaa accaagcact tattaaaaca ggtattccta tagcagatat aattgtagcg
     9121 ccaatttgaa caatacatga tacagtgtaa aattgccata gttcagtaca tatagaaaaa
     9181 gtagcaaaag caatactaga aattatacaa cctaataaat atagtatctt aatattgact
     9241 ttagaaaata atacacctat aaaaggtgat gcaattgctg aggcaatagt accaatagtg
     9301 aatattagag aaaaagaagc taaactaaag ccttctcctt ctacaacata acttataaat
     9361 tgaggctgta tacttgctac aatactaaat ggaacagctt gtattaacat acaaccaagt
     9421 actataaccc aagcatttga aaagttttta atgctaaatt tattgtttag taatgtattc
     9481 atggtatctc tcctttaggt tatgatttgt agatattaat ctaattattc taattttata
     9541 tattagtttt ggatagccta actatttaaa tataatttgg gtattaatat aaaaattaac
     9601 tataaaaatt gattatttta aatgtatggg gtaaattaaa gtatataaat aaatgaaagg
     9661 atggataaaa tgtcatttga aaaaataaat aataataacc tagataaaat acacaatagg
     9721 aagtgtatta tgcttgtaaa ctttaataaa aaagaattat cattaattaa aaatgtaagt
     9781 aactttgctg gaataagaga ccatatcatc ttaagtgata aaaatggaga gaatataata
     9841 agagacattt tagatggaaa tatatcaaat gaatgtgaaa atgggattaa gagtaagtct
     9901 gtaatattta ataacactcc aagtacacaa gtaaatgcat ttttagaagg gctaaaaaaa
     9961 gtgagaataa atagaccttt tacagctatg gtaacagaga catctattga ttggacattg
    10021 aataatttaa tagaaaattt agttcaagaa cgtaatgcta tgtctttagg aaaaacaatt
    10081 gaacataagt aaatttaaga tatcttatag taattttaac taatgtacat tgagtatcat
    10141 attatatttt agaatagaaa attttaaatg tgggtagatg gaggagattt gagatgaaag
    10201 ttttatttac aataaattat ggaaaagaaa aatttgataa aataagagaa ttaggctatg
    10261 atgtcataca ttactctgaa gatagtattg aaaataatga ggatgtaaat acagcagatg
    10321 tactagttac atacaatcca tttgagagat tagatatttc taagatgaaa aatttacaat
    10381 atatacagac aactagtgta ggtatagacc aacttccaaa agaagaggta ttgaagagaa
    10441 atattaagat agctaataat agaggcggat atagtgtacc tattggtgaa agtattgtat
    10501 tgtatatttt agaaatatat aaaaatagtg caaaattttt taaacaacaa caaaataaag
    10561 aatggaagat ggatttttct gtatcagaat tgtatggaca aagagttgga tttttaggaa
    10621 caggtactat atcttcagaa gcagcaaaaa gattaaaagc ttttggtgta gaaatatggg
    10681 gtgtcaatac tgatggaagt agtaaaaaat actttgataa gtgttttgct actgaggata
    10741 tgaatattgt atttgaagaa tgtgatatag tagttgtagc aatgccttct acaaaaagta
    10801 cagatggaat agtaaacaaa gatatgttta aacttatgaa agatggttct gtatttataa
    10861 atgtaggtag aggaaataca gttaatcaga aagatttaga agaatgtgta gagaaattta
    10921 gaggggtagc attagacgta tttgaaagtg aacctttaaa caaggacaat aagttgtggg
    10981 agtgcgaaaa tgtcatagta actcctcata attcttgggt gtcagataaa aacaaagaga
    11041 gaacatttaa tatggtttat aataatttga aacactatat aatggagaat aaacctgtag
    11101 ataatgttgt agacatatca agaggctatt aaaaataaaa aattttgata aaaataaaaa
    11161 ctcttattag tcgtattaaa gatactaata agagtttttt ttgtaataaa tttaaagccc
    11221 aaaaagtgat atactaaaaa atatataaat aggagataat taattatagg gaaatttcaa
    11281 aataatttaa ttttaaaaat atgaagaggt gcaaaaagtg agcgtaaaag atgaaattaa
    11341 taaacttata gagacaataa actaccataa tgaaaggtat tataatcaag atagcccaga
    11401 aatatcagat tatgaatacg ataagttaat gaaggagtta atttctttag aagaagaaca
    11461 tccagaacta aagagaattg attctccaac aaatagggta ggaggtaagc cattagataa
    11521 gttcaatcaa attgtacata aaactcctat gttgagttta tcaaatgctt tttcagagca
    11581 agatttgaga gattttgata agagagttca agaatatgtg ggagaaaatg ttgaatatgt
    11641 tgttgaattt aaaatagatg gtctttctgt aggtctaact tataacaatg gagagtttga
    11701 aaaaggagca actagaggag atggagttgt aggagaagat atttctcaaa acttaatgac
    11761 agtaaaaagc atacctctta agataaatga taaaaaagaa ctaatagtta gaggagaagt
    11821 atatatatca aaagataatt ttagaaaagt aaatgaacaa caagaagaac aagaacagcc
    11881 attatttgct aatccaagga atcttgcagc tggttcattg agacaattag acccaaaact
    11941 aacagcaaag agaccattag atatatttgt atttaatatg gaaaatatag aagaatacga
    12001 tttagaaagt cacagtgaat cactagagta cttagaaaaa ctaggatttt cagtaagtca
    12061 gagttataaa gtttgtaaaa gtatagatga agttattgag catattgaat actggacaga
    12121 taacaggggt aatttagact acgaaataga tggtatggta ataaaagtaa acaatataaa
    12181 acaaagagac gaaatgggat atacagccaa aagtcctaga tgggcaattg catataaatt
    12241 cccagcagaa aagaagaaaa ctaaaatagt agatataata gtagaagttg ggagaactgg
    12301 tacaataaca ccttcagcaa ttcttgaacc agtcagactt gcaggtacca ctgtaagtag
    12361 agctacactt cacaatgaag attatataag agaaaaagat ataaaaataa atgatacagt
    12421 attagttcaa aaagcagggg acataatacc tcaagtttta gaagttataa aagaagaacg
    12481 tactggagat gaaatagatt ttaaaatgcc agaaaagtgt ccagtttgtt cagagccaac
    12541 aataagatta gaaggagaag ctgctgtaaa atgtatcaat atgtcttgcc cagcccaaat
    12601 aagaagggga attatacact ttgtgtcaag ggatgcaatg aatatagatg gattgggaga
    12661 atctataatt acactgctat taaatgaaaa aattattcaa gatgtatctg atttatatta
    12721 tataaaaaag gaagatgtag tcgacctaga gagaatgggc gaaaaatctg ctgacaattt
    12781 aataaattca attgaaaaat caaaagaaaa tgatttatgg agacttataa atggattagg
    12841 tattaaattt ataggagtta aagcagctaa gatacttgca tataacttta aggatttaga
    12901 tgaagtaatt tctgcaacta gtgagcaact agaagaatta gaggaatttg gaagtataat
    12961 ggcaaacagc gttgtcgagt tttttaagga agacaaaaat atgaacgtta taaacaagtt
    13021 aaaaaatgta ggagtaaata caaaatcatt ggataataca ggtgaaaaag tagaaaaaat
    13081 atttgaaggt atgaaaatcg tattgactgg tacacttcct acattaaaaa gaaatgatgc
    13141 aaaagatatg atagaaaaaa gaggtggaaa agctacatct agtgttagta aatcgacaac
    13201 atttgttttg gcaggtgaag aagctggttc taaacttact aaagcaaatg atttaggaat
    13261 taaagttata gatgaagaga gatttttaga attattaaag ttatcatcga aagatgaagt
    13321 aaaagctgta ttagagagtt aaaaagtcgc ttaatttagt agaaaactac tataattaat
    13381 gatagaatag ttcagtaagc taataagatt acgaaaaatg acaataatta atatttgaca
    13441 aaattaaaaa ttataaaaat aaatttataa aaaataaaaa atatgagaaa ggatataaaa
    13501 aatgaccaga agttatgata gaagtaatga acagataaga cctgtaaaga taactagaga
    13561 ttatacaaag tatgcagaag gttctgttct tatagaaatg ggagagacaa aggttatatg
    13621 cactgcatca gtggaagaaa aagttcctcc ttttttaaga aatagtggta ctggatggat
    13681 aaatgctgaa tattctatgc taccaagagc aacacaacaa agaaagataa gagatgcatc
    13741 taggggaaaa attgatggaa gaacacaaga aatacagagg ttaataggaa gaacaataag
    13801 gtctgttgta gacttgaata aattaggaga aagaactatt tgggtagatt gtgatgtgat
    13861 tcaggcagat ggtggaacta gaactgcatc tataacaggg gcatttgtag cagttgcaga
    13921 agcaatatat aaattatata aagcaaagac aataaaagag atgccaataa caaattttgt
    13981 gtctgcaatt agtgttggta ttgtaaatgg aatacacatg ctagatttat gttatgaaga
    14041 agattctaaa gcacaggtag atatgaatat aataatgact gataagtgtg agtttgtaga
    14101 agtacagggg acaggagaag agaaaccttt ttctagagca gatttaaatt tattgttaga
    14161 gttaggtgaa aaagggaata aagaattaat aaggcttcaa agagaagctt taggtgaaat
    14221 atcagatgag atattgggta tggattatgg taatgaagta gtgattgcta ctaataatgc
    14281 tcataagcta gaagaaatag gtgaaatctt aaaagatttt gaatataaag tgtactcttt
    14341 aaaagatgtt gatttagcag gtatagagat agtagaagat ggaaaaacat ttgagcataa
    14401 tgcgttgata aaagctagaa caatagcaaa aaagactaaa cttatagcaa tatcagatga
    14461 ttctggctta gaagtagatg ctttaggcaa aaaacctggt gtttattcag ctagatatgc
    14521 aggagaacat gcaacagatg aagaaaatag aaaaaaactg ttaaaagcta tgcaaaatgt
    14581 accaatgagc aaaagaaatg caagattcgt ctctgcaata gcagtagttt ttcctgatgg
    14641 aaaagaattt gtagtaagag gaatttgtga aggaatgata ggatttgaag aaaaaggtaa
    14701 aaatggtttt ggatatgata gcttatttat agtaaaagga tatgataaga cttttggtga
    14761 aatacctagt gttataaaaa attcaatcag tcatagagca aacgcactaa aacttatgaa
    14821 acaagagttt ataaaaaggg tagtgaagta atgaaaatag gcatagtaag cgacacacat
    14881 atgatgatta aaaatatgga aaaaacaata ccatatttga aagaatgtga cctaataatt
    14941 catgcaggag ataactttac tgattcaaga tatattcata gcatgactaa tgtaggtatt
    15001 attgcagtta aaggtaattg tgactttgat gctgttgagg aagaagttgt ctttgaagtg
    15061 gcaaataaaa ccatattctt atgccatgga gataagtatg gtgtaaaata tggaactaat
    15121 atgttagaaa aaaaggcaac tgaagttgat gctgatatag ttatatttgg ccatacccat
    15181 accccattta gagaaattaa agatggtgta ctttacataa atcctggaag cacttcactt
    15241 ccaagaggag tatcgtataa aagttttgtc attatggata tagaagaaga tgacataaaa
    15301 attgaagaaa tacgcatata aaggtgtatt tatctaaaaa aatggcaaaa aacgctcaaa
    15361 aaattaaatt ttatcaaata agtacaacga tattaatgga acacttgtaa ataaaaaaac
    15421 ttatgtgata taattaagtg gttgaattat aaatagaatg aattataata ccgttatcgg
    15481 gtattacata aatatcacta ggaggattag aaggatgaaa gcagaactaa ttaaaaaaga
    15541 gggtaataaa gttactttaa aaataacagt agataacaat aaatttgaag cagcagtaaa
    15601 taaggcttat aataaaagca gaaataaata caatatacct ggatttagaa aaggtaaagc
    15661 tccaagaatt gttatagaga ctcaatacgg aaaaggtata ttctacaatg atgctataga
    15721 aatattattc ccagaagttt atccagaagc tatcaaggaa ttagacatag acccaataga
    15781 caatccagat ttagatatag aagaaataag taaagacaat gggttggtta tggttcttaa
    15841 tgtagaagtt aaaccagagt ttgaattagg aaactacaag ggtatagaaa tagctaaggt
    15901 tgagaatact gtaagtgacg aaaatgttga tgctaaacta caagaaatgg ttgaaaaaaa
    15961 tgccagatta gtaagtgtag aagacaaagc tttagaagat ggagatacag ctataataga
    16021 cttcgaaggt tttgaaaatg gtgtagcttt tgatggtggt aaaggtgaaa attataactt
    16081 agttataggt tcaaacacat ttataccagg atttgaagaa caattagtag gtaaaaaagc
    16141 tggtgaagaa gtagaagtta atgttacttt cccagaagaa tatcactcac aagatttagc
    16201 tggaaaacca gttgtattta atgtaaaaat aaatgatgta aaagtaaaag aattatctgc
    16261 acttgatgat gaatttgcta aagatactag tgaatttgat tctttagatg aattaaaagc
    16321 agatgtaaga gctaagttag aagaagaagc taaaaataga gcagatgcag aaactagaaa
    16381 ttcagtagta gaaaaagttg ctgaaaatac agaaatagaa ataccagaag ttatgataca
    16441 acatcaaata gataatatgt taaatgaact taactaccaa ttacaatatc aaggatttgg
    16501 attacaacaa ttactagaga tgactggtag aactatggaa gaattaagag aagagagaaa
    16561 agaagatgct aaaaaattag ttaagtcttc tttagtatta gaagcaataa ctaaagctga
    16621 aggaattgaa gcaacagaag aagaatttaa ggctgagtta gaaaaaatgg cttctgctta
    16681 caatatggaa gtagaaaaaa tagaagcttc tttaagagat gctgacaaag aagatataaa
    16741 aggtcaaata aaaataagaa aaacaataga tttattagtt gataatgcaa caatagctta
    16801 ataaaataaa cgctaactaa tatagttgac gttatgaaat gtttagtata taatgatgtg
    16861 caaatataat ttagttaaat gcgtataata atttacagta gcttaaaaaa agctactgta
    16921 aattatatag aataaagggg gtatgaaacc atggcattag tacctgtagt tgttgaacaa
    16981 acaggaagag gagaaagatc ttatgatatt ttttctagac tattaaaaga cagaataatc
    17041 ttcttaggag atcaagttaa cgatgccaca gcagggctta tagtagcaca gctattattt
    17101 ttagaagctg aagacccaga caaagatata catttatata taaattctcc aggaggaagt
    17161 ataacttctg gaatggctat atatgatact atgcaataca tcaaaccaga tgtaagtact
    17221 atatgtatag gtatggctgc ttctatggga gcattcttgt tggcagcagg agcaaaagga
    17281 aaaagactag cactaccaaa tagtgaaata atgatacatc aaccattagg tggagctcaa
    17341 ggtcaagcaa cagatataga aattcatgca aagagaattt taaaaataaa agaaacttta
    17401 aatgagatat tatctgaaag aactggtcag ccattagaaa aaattaaaat ggatactgaa
    17461 cgtgataact tcatgagtgc actagaagcc aaagaatatg gtttaataga tgaagtattt
    17521 acaaagagac cgtagataga gagaggtgtt aatatgtcta aatatgaaga aaaacgacaa
    17581 ttaaagtgtt cattctgcgg taaaaatcaa gatcaagtaa gaagattaat tgcaggtcca
    17641 aatgtatata tttgtgacga gtgtgttgaa ctttgtgacg aaattattca ggaagaaata
    17701 gaagatacta tagatgaaga tactacatct ttaccaaaac caaaggaaat gatggagatt
    17761 ctaaatgatt atgtaatagg tcaagaaaaa gctaaaaaag ctttatcagt tgcggtttat
    17821 aatcattata agagaatata cagtaaaaaa tctagtagta aggatataga aattcaaaag
    17881 agtaatatac ttttattagg acctacaggt tctggaaaga cattacttgc tcaaactcta
    17941 gcaagaactc taaatgtacc ttttgcaatg gcagatgcta catcattgac tgaagctgga
    18001 tatgttggag aagacgttga aaatatactt ttaaaactta ttcaagctgc tgattttgat
    18061 atagaaaaag ctgaaagagg tataatatat atagatgaga tagataagat tgcaagaaaa
    18121 tctgaaaacc catctataac tagagatgtt agtggtgaag gtgttcaaca agcacttcta
    18181 aaaatattag aaggtactgt tgcaaatgtt ccaccacaag gaggaagaaa acatcctcat
    18241 caagaatttt taaagataga tactactaat gtattattta tattaggtgg agcttttgat
    18301 ggattagaga agataattca aaaacgtggt ggagataaaa cactaggttt tggagctaaa
    18361 atagaaagta aaaaagaatt agatttaggg aagctgtatg aaaaagtact tccagaggat
    18421 ttattaaaat atggtataat accagagttt ataggaagaa taccagtttt agctacttta
    18481 gagttgttgg atgaagatgc attaatgcag atattacagg aacctaaaaa tgctctagta
    18541 aaacagtata aaaagttatt ggagttagat gatgttgagc tagaatttga agaaggtgct
    18601 cttcgtgcaa tcgctaaaaa ggccatagaa agaaatacag gagctagagg acttagaagt
    18661 atagttgaaa gcgtaatgat ggaaactatg tttgaagtac catctagaga taatataaag
    18721 aaggttatag taacagagaa atctgtgaac gaagactcag taaatcctat catagtactt
    18781 aaggatcaag aggaaagtgc ataagatgat gaaaaggagc tttaaaagct ccttttttta
    18841 tgttaatatg ctaaatataa aataggtatc attattataa aaatgtgagt aatataccta
    18901 gaataccaaa gacgaataca attgcaatgg aaccaatttt tcctttatat aaaagataaa
    18961 atgatgctat aaatgcaatt attccatata aatcccaatt aacttctttg gcagatgaaa
    19021 aagctgctga taatatgaat cctagagtaa ttggtctaag aactgtcaat aagttttcta
    19081 cctttttatt atttttaaat ttagaaaata actttgatat aatagttaga cctattaatg
    19141 caaaaattgt aacaccaata gttgcagcta gagaaccaaa gaagccagca gtcttaaatc
    19201 ctacaaatgt agcactattt attgctacag gacctggtgt agattgtgat atagcaagta
    19261 aatccaaaaa ctcttgatta gttataaagt gatatttaga tataaattct tgctggataa
    19321 atggtagcat ggcataaccg ccaccaaaac taaatgcccc tattttaagg aatattaaga
    19381 agagtagtat tatttctgac atagactata tacacctcct ccaattatta aataaatagg
    19441 gcttatattg aatacaccaa cagataaaac tgctataatt aatagtagta tgttagtttt
    19501 agactttggt agtttcttga acatgctcgt aaaagaatac aatataagag ctattacaac
    19561 aggcgcaact cctttaaaaa atccatctaa aattttagat gatgaatttt taaagtatag
    19621 atacgataag actaagacta tacaaaatga tggtaaagca gagcctaagg tacatataag
    19681 aactcctggt ataccataaa gtttatatcc taataataca gatatattaa ctgccagtac
    19741 tccaggataa ctttgagcta tggataaata atcaataaat tcttcctgct ctaaaagttt
    19801 ttgtttgttt acaagttcat cttgtataag aggaaccata gcgtatccac caccaaatgt
    19861 aaacgcacca atttttaaaa aggttaaaaa caatttcaac ataatagaaa cctccaaaag
    19921 ataaataaca tctaagtata ctaatattaa tattatatca taataaaaag ataatatagt
    19981 atgatagtat tctaagaatt tatcattaat gttgaaaatt aacttgttca aatatataat
    20041 attaaaagca aagcaattag actagaaaag gagtaatgtt gatggaacaa aattatacta
    20101 aaatagatca tgaattacca ttaattcctc taagaggatt agctatattt ccatatatga
    20161 tattaaattt tgatataggt agagagatat ctcttaaagc tttagaccaa gctatgatgg
    20221 atgaagaact tatattttta acttctcaga aagaagcaga ggtagatgag ccaggagaag
    20281 aggattttta tcatgtggga acaatctgta aagttaagca aatgataaaa ttaccaggag
    20341 atacagttcg tgttttggtt gaaggtgtat caaggggtag agtaaagaaa atagaacaag
    20401 aagatgggta tttcagagca gtaatagaag aaatagtatt tgatagtgat aatttggata
    20461 gtgaaacaga agttgaaatt gaagcatttg taagaaatgt atttgatgcc tttgaagagt
    20521 atataaatat tggaaatagg gtatcaccag aaatattaat atctttagct gatatagaag
    20581 atgtagatag atttatagat acgatagctg caaacatata tctaaaatca agccaaaaac
    20641 aagaaatatt agaagaattt gatattagaa aaagacttga acttatttat tctatattat
    20701 tagaggaaat agatatatta aaaatagaaa agaaaataac tttaagagta aaaaaacaga
    20761 tgaataaggt tcaaaaagaa tattatctta gagaacaatt aaaagctata cagaaagaat
    20821 taggtgaaga agaagatata aactctgaag cagatgagta tagagaaaag cttaaaaaaa
    20881 taaaagcacc taagacaact aaagagaaaa tagaaaaaga gatagataaa ttttcaaaaa
    20941 tatcttctat gtctccagat gtatctgtaa gtagaaacta tttagatact atattctcat
    21001 tgccatggaa taaagagaca aaagataaat tagatataac taaggcaaaa gatatattag
    21061 atgaagacca ttatggatta gaaaaagtta aggagagaat actagagtat ctagctataa
    21121 gaactcttgc taagtcactt aaagggccta taatatgtct agttggtcct ccaggaacag
    21181 gaaagacatc tatagtaaaa tcaatcgcca gagcattaaa tagaaaattt gtaagaatat
    21241 ctttgggtgg agttagagat gaagcagaaa taagaggtca tagaagaact tatgtaggtt
    21301 caattccagg aagaataatt aatggggtaa aagaagccca aacaaaaaat cctgtattct
    21361 tatttgatga gatagataag atggcagcag attataaagg tgacccagct tcagcaatgc
    21421 tagaagtatt agacccagag caaaataagg attttgtgga ccattactta gaaatacctt
    21481 ttgatttgtc aaaaatacta tttgttacaa cagcaaatag tctaagtaat ataccaagac
    21541 cattattaga tagaatggaa gtaatagaag tatctggata tatagaagaa gaaaaattaa
    21601 atatagcaaa aaaataccta cttccaaaac aaattaaaga acatgcttta aaagaaaact
    21661 ttataaaaat agatgatgaa actttaagaa gtataatcaa ccattataca agagaagctg
    21721 gtgtaagaac actagaaaga actattggta agatttgtag aaaggttgcc aaaaaatacg
    21781 ttgaagaccc aactcttgaa gaggttgtta taaataagtc tgacctagag acatatcttg
    21841 gaaaagacat gtttaagtat caacttgcag aagttaaccc tcaaatagga ttagtaaatg
    21901 gtcttgcatg gacagaagtt ggtggagtta cacttgaagt agaagtaaac gtacttaaag
    21961 gtaaaggaga aattgtatta actggaaaat taggagatgt tatgaaggaa tctgcgaaga
    22021 caggtatctc ttatataagg tcaatagtgg acaagtttga tatagaccct gaattttata
    22081 agacgaatga tattcatata cacattccag aaggtgctgt tccaaaagat ggaccatcag
    22141 ctggtataac tatggctctt gcagtaatct cagcacttac taaaagacct gtgcctggaa
    22201 atatagcaat gacaggagag ataactttaa gaggtagagt cttagcagta ggaggagtta
    22261 aagaaaagtt attagcagct catagagctg gtataacaaa agtattaatt cctaaagaat
    22321 gtgaagctga tttagatgaa ataccagaaa atgtaaaaga aaaaatggag tttgtgcttg
    22381 tagaacatat ggatgaagta ttagaacaag ctttattaaa gagtggtgaa aataatgaaa
    22441 attagaagtt cagagataac tatgagtgct gtgaataaat cacagtatcc agcagaagga
    22501 atcccagaga tagcattggc tggtaggtca aatgttggaa aatcatcaat aataaataca
    22561 ttgcttaaca gaagaaactt cgctagaacc agtcaaacac caggaaaaac tagaactata
    22621 aatttttatc taataaataa tgaattttat tttgtagacc ttccaggcta tggatatgca
    22681 aagatagcta agtctgaaaa agaaaagtgg ggaggtataa tggaacgata tctagaaagt
    22741 agacaagagt tatgttctat attcctactt gtagatatta gacatgagcc tacagcagat
    22801 gataagctta tgtatgaatg gataaagcac tttggatata actgtgtagt tattgcaact
    22861 aaggcagata aaatatctag aggacaatat caaaaacaca taagtataat aagaaaaaaa
    22921 ttgcaaatgg aaagtagtga aaaagtaatt cctgtttctt cactaaaaaa gactggtgtt
    22981 gaagaattat gggaagagat tgtaaatcaa tacaatcaac atggatatga aataacagta
    23041 gattaaattt aaaatgttaa atattgacat gagatttatt tgaataataa atgtaataat
    23101 agttagtttt aattatatta atattaaagc aaaatatagg atgccttaaa atagttttaa
    23161 tgaacttttt ttggcatcct tattgtttat aaacaataaa tatcagtaat tttgttaaat
    23221 actaataaat tttagtaagg tatgggaaaa ttagtataaa ttagttcaac taaaaaatta
    23281 ttgacttatg tagtcagcaa taaatagcat ttaagaaata gatttatctg tttttctcat
    23341 aaagaaaaca gttagtataa gccctataaa acatactgat gctgcaatcc aataagactg
    23401 attgctagct ataacatagg ctgatgtgtg acttttaaac ttttgattgt atgtaagact
    23461 ttgtgcaaaa aaactactaa aaaatgctgt tccaagagca aatgataaat tacgaatagt
    23521 tgcaagaaaa ctgcctccgt atccctgttt ttgaggagga acatctgcta aaatactact
    23581 attgtttggt gaactaaatg ctcctagccc aagtccaaaa ataactaaac atacaattaa
    23641 aagatacaat ggagaatctg cttttaaaag tgccattcca actagtgaga tggtcataaa
    23701 tgaaaaagat gctgttagaa tgttttttgc accgtgttta tcagaaagtg aacctgcaat
    23761 aggtgctata agtaccataa taagtggatg tacagtcatt acataaccag ccatcatagg
    23821 agataaattt tttaattgtt ctaaataaaa tggaagtaga aaagatactg ccatttgagg
    23881 aaaataacaa gctactccaa tgatattgcc tacagtaaag tttctatttt taaatagagg
    23941 taggtcaata aagctttgtt caaattttac ttctcttaat gccaacaata agaagattat
    24001 aagtgctgtt atacctaaat acaatcttgt attacccttc tcattcattg caagtaatat
    24061 aagcgtagtg caagatgcga aaagtagaag acctggaata tcaaagctct tgttttgttc
    24121 atccaaaaca ggtttaggta gaaatttaat gccaagaaag actgctataa taccaatagg
    24181 tatattgata ataaaaatag aaggccatcc aaaatgttct aaaattatac ctccaattac
    24241 aggaccactc atatttccta taccaactac agctccagtg attcctatag cttttccttt
    24301 ttcatttgct ggaaatgcat tggaaactat accaagccca gtagccatta aaatacttgc
    24361 tccaactgcc tgaaagattc ttgaagaaat taatgttgat aaattgtttg acatactaca
    24421 cagaaaagaa cctattgtaa atataaaaaa tcctaatgtg tatagacgat ttgaacctac
    24481 tttatcgcca agttttccga aaaataacat agtagctgtt acaacaagca tataagctgt
    24541 tacaacccac tgaatttgtg acatgttaat tttaaagtca gcagcaattg taggatttgc
    24601 gatatttagt atacttccat caagagtaga cataaataca ccaatacaga tgataatcaa
    24661 tactatccat ttttgtttca tgataacact ccaatcttaa tagattttat aagtacgata
    24721 taattgtaaa ataaacaaca aaaaaagcac ctgtatatta ggtaccttga atgtgtctaa
    24781 tttcatgaat aaatacggta gttgaaggat ttgtatagat tactcatgta aaattacata
    24841 aaggtactca tatactattt tcttctacca tccagatttt actgtcggct ttggaatatg
    24901 accaaattat gccataaggc tcgcgggctt ttaccgccga tagggaattt cacccgttta
    24961 tgaagaaaaa cagatgtatt ttgtaatatt attttaacat aaaattataa tggaaacaat
    25021 tggatttaag taatatccag ctgtattaca atacaaatat ttcaatatgg acacttaaat
    25081 tgtatttata gatagaataa ggaacagtat gagaatctat aataattaga taaaattaac
    25141 ctgttattag tattagatgg taagttttaa atttatcaaa atcagaaagc tgtaataaaa
    25201 ttcttataat atgaatatct taattaagag atattgtaaa ttatgaggag gcgctaatat
    25261 ggaaggaaag gtaaagaagg cattcccggg tggaaataca tcacaaggct catattcttt
    25321 ttttgactat ataattcctg aaaatgtaaa aagagttttt tgtttaaagg gtggacctgg
    25381 agtaggaaaa tcctcactta tgaaaaaaat ttataaagag tttttgcata gaggatatga
    25441 tattgattta tatcattgtc cttctgaccc aagttcgttg gatggtttag ttataaagaa
    25501 attaggcgtg gtacttatgg atgcaactgc cccacatact atggacccaa agctacctgg
    25561 agctttggat gagataatta atttaggtga ttactggaat acagaagaat tagaaaagaa
    25621 taaacaagaa atatcagttt atgataaaga tattagtaat tcatttaaaa gagcatttaa
    25681 gtttttaaaa tcagcagaac caatctttag agacatagaa gaaaaatatt ctgattgtat
    25741 ggattatgga aaagtaaatt tggtcacaga agaatttata aaaaggttat ttgatggagt
    25801 taaaagcaca ggaaatctaa aaagagaaaa gcatttattt ggttcagcta taactcctgt
    25861 agggtgttta gattatacag aaaacatttt aaaagatgta aagaaagtct attatttaga
    25921 tggagaaata ggaactggga aaactacatt attaaacaaa gtatataaga aagctattga
    25981 aaaaggttta aatgtagaag tttatcatta tccacttatt ccagagaaaa ttgaaactgt
    26041 actattaact gatttagata taggcataac tataagtacg acttttaaag aagaagatac
    26101 aattgattta aatcagtttt taaatagaga aaaactactt aaatatgaag aagatattaa
    26161 atttgatgaa aaagttttag atgatttaat ttgttgggca gtactaaatc ttaagagagc
    26221 caagtctaaa catgatatta tagaaagtta ctatagtcct aatatgagat ttgatgaagt
    26281 agggaaatta agagataagt taattaaaag aattttaaaa tatgaggaaa acttataaac
    26341 gcatatgttt aaaattaacg ttagtaataa tataattttt agtaaattgg tgtagctttg
    26401 aggctatgcc attttacttt ttttgctaaa aaatgatatt aataatctcc aaagccatta
    26461 tttgtttttc ataattatta tttcctttcc taatttatgt ttttttataa tatcacaaca
    26521 atgtttattg aaagtttata taattttaag aacatctaaa aatgcatact tgatattggt
    26581 aaattagtga taggagttca atgtatgata ttatatacta aagatgaatt ttaatatata
    26641 actttgatat aataagcata tgtaaatgtt taatatttaa ttaaagaaaa ttatcatata
    26701 aaaataaatt tgtaaggagc aatacagtat gaaaaaacaa tatgtgttta taatactagc
    26761 aagtactatt ttaggtatac ttgttggaac catatttaga gattatagca atgaagaaac
    26821 agtttataca agcaaagaca agtcaataag aaaagaaata aaaatgacaa gaaaatctat
    26881 aaaagaatta accaaagaga aagacaagtt agataaagaa attaattcaa tgaaacaaaa
    26941 tcaaaaaaat tctgaagaaa tatcagaaat agaaacactt agagatagtc tatcatattc
    27001 agatgtaaag ggtagtggga taatattaag tatagatgct atagatgaaa aaacagggaa
    27061 tatagctaat agtatagact ataataagat tcttgtaaaa gttataaatg aattaaaagt
    27121 aaacggagga aaatacatag aaataaatgg tcaaagaatt aatcaatatt cagaagttgt
    27181 acttgcagga agccatataa atataaatgg agttcctgta gcacaaccat atgaaataaa
    27241 gtgcattgga gataaggaga aactatctaa ttatgtagat aaaggtagta aatatttaga
    27301 tagcatacaa accaattatc caatgaaaat aagcataaaa actgaagaaa atatctctat
    27361 gaaaaaaatg aatataccta ataaattaaa gtatataaag ggtgattaag attatattag
    27421 aggaaagcag tttgccaatg ttaataccag aaaaacttgc aaaaaaatat aatgtatttc
    27481 cagttgagtt tcaagatgat aatttagttg tagaaataga agatgaggat atttatgctc
    27541 tacaggactt gagattagct acaggtaaag aaattatatt gaagaaggag aaaaaagaac
    27601 ttataagtga aaagattgaa aaatattata atagctttga acttgatgag gattatgcaa
    27661 aaaaattatt tgaaaatctt ttagaaaaag ctataaaaga aaacgcaagt gatatacata
    27721 tagaaccatt taaagaacat ttaatagtga gaatgagggt agatggagaa ctaaaggaag
    27781 tatcaaagtt tttagttgat gtctatccat cattgtctac ggttataaaa ttaaaggcat
    27841 caatggatat aacagaaaaa aggcttcctc aggatggtag ggtggatata aagttaggtg
    27901 gaaatttaat tgacattaga gtttcatcaa taccaactgt ctatggtgaa aaaatagttt
    27961 tgaggatact taatagagat acttttttaa aagacaaatt agaacttggt ttttcagagg
    28021 aagctataaa atctataaat cgtataataa ataaaagagc aggaatatta ttagtgacag
    28081 gtcctacagg tagtggaaag actacaacag tatattcctt attaaatgat ttaaaaggaa
    28141 taaataaaaa tattatgact atagaaaatc ctgtagagta taagatggat ggcataagtc
    28201 agattcaagt aaattcaaaa gtagggttga gctttgatgt aggtcttaga gcgatattaa
    28261 gacaagaccc agatataata atggtcggag aaataagaga tgcagaaaca gctaagatag
    28321 cagtaagagc agctataaca ggtcatttag ttataagtac tttacatact aatgatgcag
    28381 tatcttccat agctagatta ctagaaatgc aaataccacc atatctttta aatgcctctc
    28441 ttataggtgt tatatctcaa aagttggtta gaaaagtctg caatcattgt agccatgaga
    28501 ttatgataaa agataatttt agtggagatg taaatactaa ggtagctgta gggtgtgaaa
    28561 aatgtaatga taaaggatac tttggaagaa cagcaattta tgaaatctta gaaattaatg
    28621 atgatataaa gacatgtata agaaatatgg aggattctag tactattaaa gatgtagcac
    28681 aaaataatgg aatgataaca tttgaagata gttgtaaaag actaataaat gaaaaaataa
    28741 cgacattaga agagtgttta gtagtaaatg aaatgattta actgaattgt atagttaaac
    28801 ttcgtttggt cagttgtctt tatttaatct attttaatta agatgtttga gtataaatta
    28861 tatttaagtg aaataaaatt aaatgcatta aattagaaaa tattaagttt atttaattgt
    28921 aattaatata atttggaagg tattatagta catgagatta tctaaaaata tattttataa
    28981 tttagaaaag ttttatttag tattaaatag aatcagtgat aaagaaatta gagtaatttg
    29041 caaagagata ggcatattgc tggaatctgg atgtgaaatt acaaaaatat ttgaaatcat
    29101 tgagagtcag tcaagtaaaa aagtaaagaa cttgcttagt atagtatcaa atcatattca
    29161 aaaaggtaac tcaatagcag aatcatttca aataacaggt atattttcta aattctttat
    29221 aagtatgata aaagcaggag aaactagtgg aaacttagat ataataatga gtgatttatc
    29281 taattattat gataaagaat acaaattaaa aatgaaaata ataactattt caatatatcc
    29341 aataatactt ataatattat ctatcttatc aatgttgttt atattcgtat ttgtaatacc
    29401 taattttcag gtggttttta ccaacaatgg aattgaacca cctttaataa ctagagtttt
    29461 aatgggaata tctacagtgg ttacaaataa cttagcttat ataatattta gttttatatt
    29521 gttttcaata ggaagcattt atttctttat aacaaatgat aatataaaaa aattgattaa
    29581 ttcgttaaaa ttcaaaatac cttttataaa aaaaattaat cagttagtag ctacaacaag
    29641 attttcaagg gcattgtccg ttttaattag cagtggaata caaattgtgg aagctataga
    29701 tatgtcagct agtgttatag acaacgaatt tatttatgaa aggttaaaga tttctaataa
    29761 ttatataaaa aaaggaaata gcataggtaa atctttgaat ttagcaaata tttttccaaa
    29821 attatttatt tcaatggcaa atacaggaga agaaagtggg aaactagata aaagtctaaa
    29881 tgtaataaat aaattttatg aaaatgaatt aaataacaaa atagagcaaa taatgaaatt
    29941 tatagaacct atgctaattg tatttatggg attaattatt ggtactttta ttttagcaat
    30001 ggttgttcct atgtttgatg caattactaa tttttagaga gaaaaataaa ttttattaga
    30061 tgaaactttt gaaaaggaga gaatatttaa ttgataaatt tgataaataa aaaacgaaaa
    30121 ggatttacac ttgttgaaat gattgtagta gtaactattt taggcgttat atctagtata
    30181 gcattagtta agtatagtaa ggttcaagaa agtgccaaat taaatgcaga ctatacgaat
    30241 gctgctaata tagtaaccgc agctagcatg gcaattaatg atgatgaaaa gacaatagac
    30301 tctctaagtg tagagacatt gaaggaaaag ggatacctaa atactgttcc agttcctcag
    30361 agtacatcag gtaaattcga acttgtcata aatgatagcg gaacagatat aagcgtaaat
    30421 ataaattcta agcaatttta tccaaaataa tattttatat tgattgctgt aagttttaaa
    30481 attattttta tttaagaagg agatggctat aattaaaaaa attctaaact tagaaataaa
    30541 tgatgattat ataaggcttg tatttttaag aaaaagtcat aaaaaagttt ttatagataa
    30601 atcaattata aaagagatta actttgatat aaaaaatgat agtaattcga ttgagaagat
    30661 taaatatata gaagatgtag ttagatttgt aaaagaggag attggtaaat caaaaatatt
    30721 atataaaaat ttatacttta acattcaaat tcaggatata gtcattagaa atgttgaagt
    30781 tctaaatact aagaaaaaaa gagacatatt aagcatgatt gaatttgaaa taacacaata
    30841 cattccaata aacataaata actattcaat aaaatataaa gttataaatt ctaaaaatga
    30901 taaattgaat atacaagtaa ttcttttccc aaagaatatt attcaattaa ttaagaacat
    30961 ttcagaaaat ctaaatatga atccaaagac tataaatata aattttgata tattacaaaa
    31021 gcttataaac ttaaatttaa tagagaattt ttcagaagaa ggggtattta tagaatgtaa
    31081 aaaaagtcag ttaattataa atataacaaa agataaaaaa ataagagaaa catacttgct
    31141 atcaaaagag ggaaaatcat atgagttcat aaaaagatta ttaaggggtt ttaagtatgt
    31201 ttattactat ggtatagaaa attgttttat tgaagaatac tttaaatata cagataaatc
    31261 tattgaagta aatccattaa gattaaaaga taaagtaaaa gtagaaaata attttgatgc
    31321 agatttagaa gacaatagga taaattatat aaatagtata ggaatgatta tttaagtgaa
    31381 agtggataaa acaataaatt ttcttaatgg atttaaaaaa actaaaccat gtaataaaat
    31441 ctataaaata ataatagtct taattttaac gtgtatttta tttaaatcat ttatgtatgt
    31501 gaaatctata aatgaacttg aagattatat aagtcaaaat aagctagtgg ataaatcaaa
    31561 gaacacttta ccaacaacag ataaaactag agagaaattt tctaaaataa aacttaatga
    31621 aataaaaaaa atatatgaaa caattggaat aaatagtatt actaaaatat caatagataa
    31681 aaataagcta gagatggaag gtgtgtgtag tgatttagat gttttaaaca atttaaggaa
    31741 aataaagtac ataaaatatt attacataga cagtattgta agagatggag aatattatag
    31801 atttagaata aaaagttaga tgggtgatta tttttgagat taaataatag agaaaaaaat
    31861 ctacttaaaa tattgttaat attagttatt atattggcaa gtgatatagc agtaagttat
    31921 ttggaagaaa acctaactag tttgaagaat gaaaagcaaa atataataaa taaaataaat
    31981 aatcaagata attatcaaat aaaggataat aagcatatag atgataaata tatagaaagt
    32041 gttgaattaa ataaaaatga agaattgaaa aataatttta ataactcatt tttagataaa
    32101 aaaacaaata aagatatagt gttattagta gaaaatgaac tgagagatat tgtgaatgta
    32161 gaatatatta atcaagaaag tagtttagat aatcacaata aaaaacaagt taatttagaa
    32221 ataaaagtta atggagaact tgcaaatgtg ttgaaacttg aaggtgtaat gaagaagtta
    32281 agactaaata ataaacttga aaagatagaa attaacaaat taaaagtcaa taattttgat
    32341 gttcaaaata ttccaaatga atataaacaa gaaaaagaca atattgttga atgtaaaatg
    32401 acagttagag tcatataaca aaaaagaata gataaagtaa aaaactatta tcaattaata
    32461 aaattttata gaggaattag ttttacgata attcatttct acaagaggga ttgttgatga
    32521 ttttatcgaa atcctaatta aagttaatat attgtaccaa aatataagtg aatttaatag
    32581 aatatttact tatactatag attgattaat aaaaaaatgg tataataaca aattgtactt
    32641 taatttaata ataagggagt ggaagtcata ttgttaggga gaaatgagct atgcccttgt
    32701 ggaagtggca aaaaatacaa gaaatgttgt ttaaataaag atattgtttc agaaagagca
    32761 tgcagaaaaa ctgaactttc acaaaaacaa tatacagatt tatattcaaa gttatatgat
    32821 tattcaagac aggataagtt taaagaagaa tgtgaaaaag ctaaagaaat gttttatata
    32881 atgcaaaatg atagtgtaaa tgaaaaattt gaaagatttt ttaatactta tttaatacaa
    32941 gaccacataa tggaaaataa aaaggttatg acagttggtt ttcttgaaga aaatggtgca
    33001 aacctaagtc aatcagaagt atcaatatta aaaagtatgt ttgaatcata cgtaagtatt
    33061 tatgaggtaa aagaagtttc tgctggaaaa atagtattaa aggactgttt aaatggaaat
    33121 gaactttgta cagaagatgt aaaacttctg agaagtttta aaatcggaag ttgtatgata
    33181 gctagaatag ttgatattga aggaactaac atattgatag atataactat aagtatatca
    33241 aatgaggtta aagatataat tttaaaggat attatgaatc tattcaatca atatcaagat
    33301 gtatacaaag atatgaagtc gttcttaatt tatcatacac atatactata caagtatata
    33361 caacagcttt tagacccaag tatagctgaa tatttaaaga gagaaagaga taaaaacgat
    33421 acaaaagtta attcagaaaa tgaattagct gaagaagaat gtaaagtttt agatactctt
    33481 aaagaaaatg tagaaaaaga aaatatagat gcatgtatag agttttggaa tgaatataag
    33541 aaaaatcatg aaaatataaa aggagctgaa aatggttggg cagctgctgt tgaatactat
    33601 gttaaaaaag aagcaggaca acctataact caagttcaaa tatcaaagaa atacgatata
    33661 agctctagta cactaggaaa gagatacaag gatttaaaaa tatcttaatt aattataaaa
    33721 agctttaagt catagataaa gaatctatag tataatatat gtataatgtt tagaaagagg
    33781 tattaataat gagtcttagt ttaggaaatg caattgctgt atcaacgaac tttataaaat
    33841 ctaacccagc tgtacctgtg tacaacggag aagaaatata tgcagttatt tatcatatag
    33901 aaataggata taataatggt gtatcacctg ataccaaaca atatataata atagactata
    33961 aagagtttga tttatctatg gataaaaaaa atgaactaat aaatattgct aaagagtact
    34021 gtaaatctaa aggtattgta gaaaacgaaa accttgaaat aaacctttta gacaatgaag
    34081 aagcagagaa cttcttagaa aaggtgttta ataatatgaa ggttataaga ggtcttataa
    34141 ctaaaaaagg ataaataaat ggactatata ggaatagaaa atataacacc ttatgaaaat
    34201 acatatgaat ttagtgtata tgaatatgat gatgaaatca ccttaggtag tgaaaagtta
    34261 tatgtatgtg aattaagggt tgtattgatt aaagttaatt ctctgtatgt tgaaagattg
    34321 cataaatcag ttgaagcaat ggtcttagta aaaaacttga aaaaagattt agataaaaca
    34381 cttgttgtaa acaaaataaa gaattttgtg ctagatgaga tttgggtaga aaatctagta
    34441 aaagagaata tagaagttat atttgtagaa agctaggaat aaccctagct tttttatatt
    34501 acaatctaag agaggaaaac aatatgttaa gagtatctaa tgtaaaactt ggaattgata
    34561 aagatatatc tttattaaaa gatgttatac ttaagaagct aaaaataaaa gaaaaaaact
    34621 tagtcaaata tagtatatat aaagaatcta tagatgctag aaaaaaagga aaaatggaat
    34681 ttgtttattc tgtagatgta gaagttaaag atgaaaataa aatacttaac tcaaaaataa
    34741 aagatgtaac aaaaataaag gaaatcagat acgtaaatgt acctgtgggt gataaaaaat
    34801 taaagaataa tcctttggta attggtagtg gaccagctgg cttgtttgca ggtctgcttt
    34861 tagctcaaat gggttatgaa ccaatcatac ttgaaagagg tcttgatgta gacagtagaa
    34921 ccaaagatat aagtgatttt tggacaacta gaaaattaaa aaataattca aacgttcaat
    34981 ttggagaagg tggagcagga actttttcgg atggtaaact tacaactaga ataaaagata
    35041 tacgatgtag aaaagtatta gaagagttag ttcagtttgg agcaccagat gaaatattat
    35101 actcacataa gccacatgtt ggaacagata tattaaaaaa tgtagttaaa aatataagaa
    35161 atgaaataat aagtttaggt ggtcaagtta gatttaattc aaaagtaaca gatatagttg
    35221 tatcaaatga ttctatacaa tctgtaataa taaatgatac tgaaaaaata gatacagata
    35281 caattatatt agctgttgga catagtgcaa gagatactta tgaaatgttg tattcaagag
    35341 gtataaaaat agttcaaaaa ccttttgcaa taggagctag agttgaacat ccacaagatt
    35401 taataaataa agcacagtac aaggattttt ataatcatcc taggttagga gctgcagatt
    35461 atagacttat agagcatgtt tcaaatggaa gaacagcata tactttttgt atgtgtccag
    35521 gaggtagtgt catagcatct gcatcaaatg attttgaaat agttacaaat ggtatgagtg
    35581 aacatgcaag agataaaata aatgcaaata gtgcattttt agtaaatgta acgccagaag
    35641 attttaagag tgataatcca ctagcaggtg ttcatttcca gcagaagtat gaaaaattag
    35701 cttttgaatt aggtggaaaa aattataatg caccagttca gcttgttgga gattttataa
    35761 acaataagac ttctttgaat ttaggaagtg ttgagccatc atatagacca ggatatacac
    35821 ttacaaattt aagtgaatgt ttgcctagct tcgtaacaga gactatgaga gaagcgttta
    35881 tatctcttga taaaaaattg aatgggtttg caatgtatga tgctgttttg acgggagtag
    35941 aaactaggtc atcatcacca ataagaatag taagagatga aaatacttta gaatcagtga
    36001 gtattaagaa tctatatcca tgtggtgaag gtgctggata tgcaggtggt atagttacag
    36061 cagcagttga tggtataaaa tgtgcagaaa aaataataca aaaatacacc aataatacaa
    36121 aaatttaaca taaacttaat gattttttaa tatttaacat gaatttaaca ttatcactcc
    36181 tataatttga taaaatacaa taggttataa aatttttaga tacagtaatc tctttacaag
    36241 gaggaaataa tttgaacatt ataataagtc taatgggtgg attaggtctg ttcttatatg
    36301 gtatgaacct tatgggagaa ggtcttcaaa aatctgctgg gacaaagcta aaaaagataa
    36361 taaaactact tactagtaac ctatttatgg gagttttggt aggtacagga gttacagcag
    36421 taatacaaag ttcaagtgct actactgtaa tggttgttgg tttcgtaaat gcaggaataa
    36481 tgactttaaa acaggcaata ggagttataa tgggtgctaa tatagggact actgttacag
    36541 cccaattggt atctttcgac ttaactggta tggccccagt agcattaggt gtaggtatta
    36601 tactttattt atttggaaat aagcctagaa taaaaaatat agccgaaata cttataggtt
    36661 ttggtatatt atttacaggt atggatttta tgaagatggc agtggagcca ctaagagact
    36721 atcaaggatt tactgactta cttgttacat ttggtagata tccactactt ggattattat
    36781 tgggatttgg gattacagca ataatacaaa gttcaagtgc atctatgggt atgttggttg
    36841 cactggcagc agaaggtcta gtgccattat cagcagcact gccaattctt tatggccaaa
    36901 atattggtac atgtgtaact tcactgttat caagtattgg tgctaacaaa aatgcacgaa
    36961 gagcagctat gatgcatcta atcttcaatg ttttaggaac ggtgatattc ttaatattct
    37021 tgaataaacc agtagtaagc atggttacaa gttgggaccc aagcaatgtt gcaagacaaa
    37081 tagccaatac acatacattg tttaatataa tatctgtatt gatactttta ccatttacaa
    37141 atcttattat aaaactagct ataaagcttg ttcctgatag agcaggtgat atagatgaag
    37201 atgaaactaa aactattaaa tatatagatg atagaatgat agaaactcct tctatagctc
    37261 ttgcaaatac gataaaagaa gcacttcgta tgggtgagaa ggcgaaggaa agcctaaatg
    37321 catctatgga agctttagta gaacattcaa ctgaaaaaat agataagaca tataggcgag
    37381 agagattaat caatgattta cagaaagcta ttttaaatta tttacttaaa ctttcaaaag
    37441 cacctttaga tgatgattca agagaagttg tggatacttt gtttaataca gtcaatgata
    37501 tagaaagaat tggtgaccac gctaaaaata tagctgaatt atcgcaagtt gcaatagatt
    37561 caaatatttc tttctcagaa gaaggacaat ctgaacttga tgttatgtat aatagagttg
    37621 tttcagcata tacttatgca ttagaatcaa tgagaactga taatgttgat ttagcttgta
    37681 aggttataaa aattgaagaa caagttgata taatggagaa atcttgtaga gctaatcaca
    37741 tgtatagatt gaacaataat ctatgtagta tagaaaatgg agttatttat ttagacgtaa
    37801 taagtaattt agagagaata tctgaccatg ctgtcaatat agcacaacaa gttatagcaa
    37861 agagattagg aaatgattaa aaataattaa tttaaaataa tataaaattt aaaatagagg
    37921 ataaataaaa gagaaacttt aaatgatacg tttagagttt ctttttttgt aaatttattt
    37981 gactaaaatt tttaaattta tacatagtta tagcatttag aagaatcgat ttcctgataa
    38041 taaatgatga gatatgtatg attttgtggc aaacttttaa aaaaaacacc tatattttgt
    38101 gttataatct ctttaaaacg aatatgctga aaagagggat ttaaatggga aaaataaatt
    38161 ttgattatag taaagctaca gagttttttt gtcaaaacga aatagatgtc atgcaaccat
    38221 atgtagatgt tgcgcacgac atgttacata ataaaactgg tttaggaaat acttttttag
    38281 gatggataga ccttcctaaa aattatgata aagaagagtt tgacagaata aaaaaatctg
    38341 ctgataagat aaaatcagac tcagatgttc ttttggtaat aggtatagga ggctcttatt
    38401 taggttcgag agctgctata gatatggtaa gtcattcttt taggaatgga cttaaaaaag
    38461 agcaaagaaa ggctccagaa gtatattttg tagggcataa tataagctca acatatatta
    38521 tggatttatt agatataatt gaaggaaagg atatttctgt aaatgtaata tctaaatcag
    38581 gaactactac agaaccagct ctggctttta gaatatttaa agattactta gagaaaaagt
    38641 atggtaagga agaagcaaga aagagaattt atgcaacaac agatgcaagt aaaggtgcac
    38701 ttagacagtt agctacagaa gaaggatatg agacttttgt aataccagat gatgtaggtg
    38761 gtcgtttttc tgtattgaca gcagttgggt tacttcctat tgctgctgct ggacttgata
    38821 ttgatgctat gatgaaaggc gcaaatgatg ctagagaagc atttcaaaat cctgacttaa
    38881 aaagtaatga tagttataga tatgctgttg ctagaacaat tttacataga aaaggaaaag
    38941 atgtagagtt attagtgaac tatgaacctc aactacatta tgtaagtgag tggtggaaac
    39001 agctttatgg agaaagtgaa ggaaaagaaa ataaaggttt attccctgct tctgtagatt
    39061 tctcaacaga tttacattct atgggtcaat acatacaaga tggaaaaagg cttttatttg
    39121 agacagtact taatgttgaa aactgtaaga gaaatataac tataagttca gaggaagtag
    39181 acttagatgg tttaaactat ttagcaggta agactgtaga ttttgtaaat cataaagctt
    39241 ttgaaggaac tctattggct catacagatg gaaaagtacc taatttagta ataaatatac
    39301 ctcaactaga tgagtataat tttggatatt tagtttattt ctttgagaag gcttgtggaa
    39361 taagtggata tttattggga gttaatccat ttgaccaacc aggagtagaa gcttacaaga
    39421 aaaatatgtt tgctttacta ggcaaaccag gttatgaaaa agaaaaggaa gagctagaaa
    39481 aaagacttaa ataattttaa atagttaagg tttaattaag attaaaataa tataatgaaa
    39541 tcatcgaaaa ggagtttact caaaatagat gatttcattt tttttatgaa aaattgggaa
    39601 aaatatattt tgataattta tattatatta agatagggag tggtatgaga tgtcaagaag
    39661 aaagaaaggg ataagtttag taatattagt tgctataata agttcaatac ttagtagttt
    39721 tctaacaata attttagtta aggacaattt agtaagtaaa tctacaggaa gttctacgcc
    39781 gatagttgta aatgatgatg gtaagagtca aaatatttat caagcagttg cagaaaaagc
    39841 tacgccatct gttgtaggta ttacaacaac tagtgttgat actagtaata tgtttgcaat
    39901 accaactgaa acacaaggtg taggaactgg aatcatagtt gattcaaatg gatatatact
    39961 tactaattct catgttatat cagatggtca agctacaagt gtaaatgtat tattcaatga
    40021 tggttcaaca acttcaggta aagtagtatg gtttgaccaa caacttgatt tagctatagt
    40081 taaggttgat aaaactggac taacaccagc agaatttgct gatagtgaca aagtaaaagt
    40141 tggagatata tcaatagcaa taggtaatcc tcttggatta gattttcaaa aaacagttac
    40201 tcaagggata ataagtggat tagatagaac tatacaaaca gaaaaaacta atatgactgg
    40261 tttattacaa actgatgcaa gtataaatgc aggaaatagt ggaggaccat tgttaaatca
    40321 aaaaggtcaa gtaattggta taaacacagc aaaagcttct caagcagaag gattaggatt
    40381 tgcaatacca atcaatacag ctaagagtat agttgaagaa gtaatcaaaa atggaaaata
    40441 tgaaaaagta actttaggca taaaaggtac agatgtatca aattatgaag cagcaacagg
    40501 tacaaaatta agtactgata aaggtgttta tgttgcagaa gtaatatctg gttcatcagc
    40561 agaaaaagct ggagtaaagg taggggatat tataactaaa gtaggagata cagatataac
    40621 aggtatgaat gatttaaata aaaagttgta tactttctca aaaggtgcta gtacaaaaat
    40681 aacagtaaat agaggcggaa aagcagtaac aataaatgtg aatttctaaa tatgaccaga
    40741 aagatagaga ctacatggta aagtgtagtc tctattttta tttaattttt atagtatata
    40801 catctaaatt aattaaaatg atgtattgtt tatagtttat tg
//
LOCUS       DAYASV010000021        38343 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYASV010000021 DAYASV010000000
VERSION     DAYASV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38343
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.021"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            23..1090
                     /locus_tag="ACGI68_001136"
     CDS             23..1090
                     /locus_tag="ACGI68_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFH0137584.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1260..3161
                     /gene="cooS"
                     /locus_tag="ACGI68_001137"
     CDS             1260..3161
                     /gene="cooS"
                     /locus_tag="ACGI68_001137"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006091 - generation of precursor
                     metabolites and energy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HFH0137585.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3154..3627
                     /locus_tag="ACGI68_001138"
     CDS             3154..3627
                     /locus_tag="ACGI68_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137586.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3602..4828
                     /locus_tag="ACGI68_001139"
     CDS             3602..4828
                     /locus_tag="ACGI68_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0137587.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRILKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4967..6154)
                     /locus_tag="ACGI68_001140"
     CDS             complement(4967..6154)
                     /locus_tag="ACGI68_001140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0137588.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRIVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6427..7251
                     /locus_tag="ACGI68_001141"
     CDS             6427..7251
                     /locus_tag="ACGI68_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137589.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GSNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7380..8645
                     /gene="gluD"
                     /locus_tag="ACGI68_001142"
     CDS             7380..8645
                     /gene="gluD"
                     /locus_tag="ACGI68_001142"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HFH0137590.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8858..9448
                     /locus_tag="ACGI68_001143"
     CDS             8858..9448
                     /locus_tag="ACGI68_001143"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0137591.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFIYIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9779..10912
                     /locus_tag="ACGI68_001144"
     CDS             9779..10912
                     /locus_tag="ACGI68_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0137592.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDGEIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNILPKGMEEKDVTNSAIVTMIEF
                     TYAEAPKSMREEHKINCEFLGLIPKDSDKIGVGIEYYDSLLND"
     gene            11828..12850
                     /locus_tag="ACGI68_001145"
     CDS             11828..12850
                     /locus_tag="ACGI68_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0137593.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13146..14066
                     /gene="pyrB"
                     /locus_tag="ACGI68_001146"
     CDS             13146..14066
                     /gene="pyrB"
                     /locus_tag="ACGI68_001146"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFH0137594.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14105..14797
                     /locus_tag="ACGI68_001147"
     CDS             14105..14797
                     /locus_tag="ACGI68_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFH0137595.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDNIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14790..15692
                     /locus_tag="ACGI68_001148"
     CDS             14790..15692
                     /locus_tag="ACGI68_001148"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFH0137596.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETSSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15777..16361
                     /gene="pyrE"
                     /locus_tag="ACGI68_001149"
     CDS             15777..16361
                     /gene="pyrE"
                     /locus_tag="ACGI68_001149"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFH0137597.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16575..17501
                     /locus_tag="ACGI68_001150"
     CDS             16575..17501
                     /locus_tag="ACGI68_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HFH0137598.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17553..18443)
                     /locus_tag="ACGI68_001151"
     CDS             complement(17553..18443)
                     /locus_tag="ACGI68_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0137599.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18631..19662
                     /locus_tag="ACGI68_001152"
     CDS             18631..19662
                     /locus_tag="ACGI68_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFH0137600.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
     gene            19956..20831
                     /locus_tag="ACGI68_001153"
     CDS             19956..20831
                     /locus_tag="ACGI68_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HFH0137601.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            20849..22339
                     /gene="cls"
                     /locus_tag="ACGI68_001154"
     CDS             20849..22339
                     /gene="cls"
                     /locus_tag="ACGI68_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFH0137602.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            22532..22816
                     /gene="groES"
                     /locus_tag="ACGI68_001155"
     CDS             22532..22816
                     /gene="groES"
                     /locus_tag="ACGI68_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFH0137603.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            22849..24477
                     /gene="groL"
                     /locus_tag="ACGI68_001156"
     CDS             22849..24477
                     /gene="groL"
                     /locus_tag="ACGI68_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFH0137604.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTESAVADLPEKEDAGMPGMGGGMPGMM"
     gene            24739..25479
                     /gene="larB"
                     /locus_tag="ACGI68_001157"
     CDS             24739..25479
                     /gene="larB"
                     /locus_tag="ACGI68_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HFH0137605.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            25614..26746
                     /gene="larC"
                     /locus_tag="ACGI68_001158"
                     /pseudo
     CDS             25614..26746
                     /gene="larC"
                     /locus_tag="ACGI68_001158"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
     regulatory      26806..26907
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            27128..28663
                     /gene="guaA"
                     /locus_tag="ACGI68_001159"
     CDS             27128..28663
                     /gene="guaA"
                     /locus_tag="ACGI68_001159"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFH0137606.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            29241..30632
                     /locus_tag="ACGI68_001160"
     CDS             29241..30632
                     /locus_tag="ACGI68_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0137607.1"
                     /translation="MRNKIIIGITFLLVLLMSGCASKQTAEINVLATTDLHGVIPYEL
                     TSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGDNNTESYIETPISK
                     DMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYII
                     KNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVE
                     DAKKWVSIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVDGIDAIVAGHNHVQ
                     IEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDKSSKLIKFEKNQES
                     DKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISS
                     PKSDGMVQIVVMNNNKPVSYIYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEK
                     GKQEYSINLKHIQ"
     gene            complement(30780..31088)
                     /locus_tag="ACGI68_001161"
     CDS             complement(30780..31088)
                     /locus_tag="ACGI68_001161"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0137608.1"
                     /translation="MLREQGSAIRDTLDSVLSLSNQKAYPVWESVNSFALIKAAQAGL
                     GITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNITQPLKIILDNISNVL
                     "
     gene            31125..31646
                     /locus_tag="ACGI68_001162"
     CDS             31125..31646
                     /locus_tag="ACGI68_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HFH0137609.1"
                     /translation="MFSIGGGYAIIPLIQEKVVNQTGWISEKIFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIMSGILGIFFYL"
     gene            complement(31775..32011)
                     /locus_tag="ACGI68_001163"
     CDS             complement(31775..32011)
                     /locus_tag="ACGI68_001163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137610.1"
                     /translation="MLECFIAKDSKIKDGVNIPTLVFKTLHDRYFKTQYFDSYYNSLK
                     CKKKLVEINNVHNSYYIDSKKFCQEAYDWFVENQ"
     gene            complement(32321..35749)
                     /gene="uvrA"
                     /locus_tag="ACGI68_001164"
     CDS             complement(32321..35749)
                     /gene="uvrA"
                     /locus_tag="ACGI68_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0137611.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEALNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKAINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNHLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQAPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            36068..38302
                     /locus_tag="ACGI68_001165"
     CDS             36068..38302
                     /locus_tag="ACGI68_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0137612.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
BASE COUNT        14819 a         4193 c         6710 g        12621 t
ORIGIN      
        1 attaatacaa aggagattta ctatgaaaaa taaattagtt tacatatgta ttatgagtct
       61 attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga
      121 ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga gaacctgtga aaaaagatat
      181 aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat catattatgg
      241 cagttttgac catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt
      301 aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac
      361 agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat
      421 tttaaaatct ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt
      481 tgaagaagga atgaaagata cacagtttat attggacgag aataaaataa attattttgg
      541 attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa
      601 tggattatca actgaatata atgaaaaaaa tttaaaacaa atggaagatg atattaagtc
      661 cttaaaaaag aaatcggatg ttgttgtagt gtactttcat tgggggatag aattagatta
      721 ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt
      781 agttatgggg tctcacccac atgtgattca aggaatagaa aagtataaaa ataagtatat
      841 agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag
      901 ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc
      961 aaatataata ccaacttcaa taacatctag taactccaga aataattatc aaccaaaaat
     1021 attggatgga agtgagaagg atagagtttt aaataaactt gaaaaaataa gcaaagattt
     1081 aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg
     1141 tttagggtat ggtaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca
     1201 attataataa agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa
     1261 tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag
     1321 cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt
     1381 ttggacttca gggggtttgt tgtagactct gttcaaatgg accatgtaga gtaactccta
     1441 aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa
     1501 gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt
     1561 taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag
     1621 cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag gttgcagaca
     1681 aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag
     1741 cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt
     1801 cagaagtttt tgatgcaata gtaaaatcat caacaaattt aaatagtgac ccagtagaca
     1861 tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta
     1921 atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta
     1981 atgtaataga cccagactac ataaatataa tgataacagg ccatcaacat tcaacatttg
     2041 caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa tctgttggtg
     2101 caaaaggctt taagctagta ggatgtacat gtgttggtca agatttacaa ttaagaggag
     2161 aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt
     2221 tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg
     2281 aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag
     2341 cgaatgcaga atacataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa
     2401 tagaaaaagc attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa
     2461 aagaccatgg atttgaacaa tctttaactg gtgtaagtga aaagaacctg aaagcgtttt
     2521 taggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg
     2581 cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac
     2641 ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag
     2701 gattagaaaa tgttggtctt atgtcacctg gagcagaaga attagcagga gaaaacttaa
     2761 aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta
     2821 taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac
     2881 aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag
     2941 cgtttggtct tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag
     3001 gaaaattggt gacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta
     3061 tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa
     3121 gaaaaaattt agggttaaag gatgtggaat gcaatgcata gaatttttat aaataaagat
     3181 ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat
     3241 gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta
     3301 gatagtaaac ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt
     3361 gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat
     3421 gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta
     3481 aagccagatg atagaagcaa gcagaacata cttaaatgtg atttatgtaa agatgaggag
     3541 tatcctagat gtgtagctaa ttgcccaagt ggagcaattg aattacagaa ggaggaaaat
     3601 gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag
     3661 agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaacg tatattcaag
     3721 atgtatgttg catcacgtta tatctgaaca cagaatttta aaacaaataa attttgttga
     3781 tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat
     3841 agacataaat aaaaaggtag ttcagacaga agacattact gtaaattatg ataaactttt
     3901 aatagctaca ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaattt
     3961 tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc
     4021 taaaaatgta gttataatag gtgctggttt agttggtata gatgcattag tgggattatt
     4081 taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca
     4141 attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt
     4201 ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg ttacaggtgt
     4261 tgcattttca aatggtgaag ttttagatgc agacatggta attgttgcta caggggttaa
     4321 accaaatgct gactttttag atggaacagg aatagaatat gatagaggta taataataga
     4381 tgatatgtgc caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa
     4441 tgctatttgg cctctggcag taaagcaagg aattgtagct gcctataata tggttggaaa
     4501 agataagaag atagaagatg aatttgcttt taaaaacagt atgaatttca tggatatacc
     4561 aactatttct ataggaatga atacacctgt agatgatagc tataaagtgc taactagaca
     4621 tggaattaat gattataaaa agtttgtttt taaagataat gtaatctatg gagcagttat
     4681 acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat
     4741 atatgattta gaaaatagga tatttgatat atgttatgca gactttttta atataaaaga
     4801 aaatggagaa ttttgttata gtgtatagta tatacgtgga gtaaggttta taaatacagt
     4861 aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt tagaaatagc
     4921 tctatttttt gtaattaaat ttaaattata ttcaatttgt aaaatattat tttgaacaat
     4981 tatacatata ctctctagtt agaggtaaat cattttttat acctttagtg aaaagtattt
     5041 gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg tataaatccc
     5101 acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc tcagagaaat
     5161 ttttttgcca gtatagtaag gtttttacat aatgtaatct tagactttct acgtctatag
     5221 tatgatattt gtaatcacta caaatatgta tcatttctct taggctagga ataactcctc
     5281 ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat ccacttatat
     5341 agtgaagtaa aaacaatcca ccatctttta aaacagcatt tacgttttta atgaaaagtt
     5401 catagttttc tctaccaaca tgttcaagca tcccaacgct tacaattcta tcaaacataa
     5461 ggtctgattt ttttaattct ctatagtcca taagtcttac ttcaagatag tcttgaaggt
     5521 tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagt gtaattccaa
     5581 cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa ccacaaccaa
     5641 tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg tgaactttat
     5701 tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa gaataactaa
     5761 gtgtcttatc taaccataag ctataaaaat cgttgccaat gtcatagtgg gcatgtattt
     5821 ctttcttttg atgtctcatt gaatttgatg agtgcaatag atgttttaca gctgaattat
     5881 caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat aaatctccct
     5941 caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta ctttttaata
     6001 gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag ccctctccaa
     6061 ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca aatttctcta
     6121 aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct aatgtaaatc
     6181 cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt aaaatttttt
     6241 caataaattt tttatttaaa ggaaatttat tttttataaa gaaatttcat gttgtagtat
     6301 attttttgct tataaatttt ggtactggat ataatattaa actgttaatg atataataaa
     6361 tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt tataaggaga
     6421 tatatgatgg aagtatatgc tttggttggg tcaagtggca cagggaagag ctttaaggct
     6481 ttagagttcg catatgaaaa tgatatagaa tatataattg atgatgggat tttaatatac
     6541 aaaaacaagg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt
     6601 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa
     6661 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
     6721 gagagattgt caattggaag ggtttgcaag tttataaata tacaagatat aagtactaaa
     6781 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
     6841 gcagttgaga taaaaagtat ggcaagtggt cttagtataa attctttgaa gagacttttt
     6901 agaaaaggaa gtaataggaa tatggtagtt ttagaaaaaa caattataag accaactttt
     6961 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
     7021 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
     7081 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
     7141 gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
     7201 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
     7261 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaatttagga
     7321 aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg ggctttcaaa
     7381 tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg
     7441 ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg agagttatag
     7501 aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa ggatttagat
     7561 cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat caaaatgttt
     7621 caagagacga agtaaaagct ttatctatat ggatgacttt caagtgttct gtaacaggta
     7681 taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta tctcaaggtg
     7741 aattagaaag attaagtaga ggatacatag atggaatata taaattaata ggtgagaaag
     7801 ttgacgttcc tgcaccagat gtaaatacta atggtcaaat aatgtcttgg atggttgatg
     7861 agtacaataa attaactgga caaagttcta taggtgttat aactggtaag ccagttgaat
     7921 ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt actgcaagag
     7981 aagctgctgc taaattagga atagatatga aaaaagctaa aatagctgtt caaggtatag
     8041 gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact gttgtagcta
     8101 tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat ggtttagatg
     8161 gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc ccaggagcta
     8221 agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata ccagctgcat
     8281 tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta gtttgtgagg
     8341 ctgctaatgg accaactact ccagaggctg atgaagtatt tgctgaaaga ggaatagttc
     8401 ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc gagtgggtac
     8461 aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta
     8521 tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt acaatgagag
     8581 aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta agaggatggt
     8641 actaagattt actcttaata aataatagga tataattatt taagatataa aggggctgta
     8701 catagtacag cccttaaatt atgtggctta caatgaagta agctattttt gttttaactt
     8761 ataaaaaact tgataaatta tgtaatttag aatgtatttt ttaccaccac atgattacaa
     8821 attagaagag ttagattaga tttttagaca tgaacttatg cattttaaaa gcaaagacct
     8881 taatttaaag tatctagtat tatttttaaa aatagtatat tggtttaatc catttatata
     8941 tataatggaa aaatatatgg atttggattg tgaactttat tgtgatgaga gagttttaaa
     9001 aaattgtact gtacaacaaa agcaagaata tgctctaact attgtaaatt caatgaaaag
     9061 aagtacaaat ttttcaaata aatttgtagc tggattaaat aaacaatctg atattagaaa
     9121 aagagtaata aacatgttca gtgaaaaaca taaaaatggt atcttaaggg ctttgattat
     9181 aagcttgttt tcttctatta cattttttaa attcgattca actagtatta ttgaagagaa
     9241 tgatttctta gagaggtcaa gatatacgtt tgaaggaagg caagaagaca tgtttgtaac
     9301 aatagatttt atatatgcag atgtacctat aaaacttaga aaagagcatg aagaaaactg
     9361 tagagtcaat ggaatagtac caaaagattc agattcaata gaaattattc ctagatttta
     9421 taaagattgt atggatttaa aaaaataagt tagacttaat attgatgttg atattatgaa
     9481 ttttttatat ttaagcaatt gaaaatttta ttatagattc aattttatat gtgtttaagt
     9541 atgaaattta tagatagttg aaataattaa atatgattga tgcataggaa tcattactaa
     9601 gctataatta attataaggg agtttaaata aattatgagt tacacaatta taaaatgata
     9661 aaaaatagtt atctgttaca aaaaatgtat ctttttaatt aataaagata atatgaaaac
     9721 ttaaaaggca aataaactta aacataaaat actcttaaat aagataggag aaaaatgtat
     9781 gtctaacttt attctaaatg ggatttttag aacagttata gtaagtagtc taagcattct
     9841 tatgatattg attttcaaga aaaatgtatt taaaagattt agcaaaaaat ttaattatta
     9901 tatatggatg atagttgtta ttaaattatt tttaccattt acatattata cttttacaat
     9961 aaatatcttg agaagtaaaa aacacattaa tattaataac ataaatttag agggatttaa
    10021 taatgtttca actataagta ataatattat attgtacact tggattataa ctgtagtagt
    10081 ttatttaatt tacactatat ttaaatatat aaaacttaaa aatttaataa atgacttatc
    10141 ttatgatgtt gatgatggag agattgtaaa tctttataaa aatatattag aagaatttaa
    10201 tataacaaaa gatataaaac tcaaatactc ttatgaagtt gaaacaccag cattttttaa
    10261 ttcatgtgtg cttttgccac cacatgagta taaattgaaa gagttagact ggatttttag
    10321 acatgagctt atgcatttta agagtaggga cctttattta aaatatgtaa tcctgttttt
    10381 aaaaacagta tattggttta atccatttat atatataatg gataaacata tagatttaga
    10441 ttgtgaactt tattgtgatg aaagagtttt aaaaaatcga aattcagagg aaaaaaagga
    10501 ttatgcttta acaattctaa atgcaatgag aaaaggttca aatacttcaa ataaatttat
    10561 agctggactc cataagcaat ctgatattaa aaagagagtt atgaatatgt ttaatgaaaa
    10621 gtacaagaat ggtatattaa tggcattaac tttatgttta ttatcttcta ttactttttt
    10681 gaaggttaat tcaacgagta ttgttaatct taatatattg ccaaaaggaa tggaagaaaa
    10741 agatgttact aatagtgcaa ttgttacaat gatagaattt acatatgcag aagctcctaa
    10801 aagtatgaga gaagaacata aaataaattg tgaattctta ggtttaatac ctaaagactc
    10861 tgataaaata ggtgttggta tagaatatta tgacagtcta ttgaatgatt aaataaatat
    10921 tattttaaca gatattaatt ataaagtttt gaatagtaat ttgttgagaa ataaatatat
    10981 ccttataaaa atagatactt atataccaag aataaaaaat agtcaagata gtacacaagg
    11041 atatacttat aaagaactca aataagtgaa ataaattatt aattataagg tttataattt
    11101 taaaaaatta gataaattca ggtgtaaaat actataaaac acaattcgga aaagtgtatg
    11161 ttgaacttca tttaaaatgg aatttcaaag ttgctatagt aagtagttta agcattcttc
    11221 ttatattaat ctttgagaat aatgtattta aaaaagttag taaaaaattt aactattgta
    11281 tattaatgat agttgctatt gatatattat ctaaaaaaat taagtatgaa aagattactg
    11341 ctaggacaat cttttcatac ttaattttta atatttatat tatccaaaat tttcatcttt
    11401 ttaacttaga atttgagaat cactattagg aaataggctt tgatttatgt agggttaaaa
    11461 atttcacaga atatctaact ttatactaaa tccatcaaaa aatatacgcc atataaaagc
    11521 gcataaaaaa acacataaaa caatatttat gcgcacttta acctcgaatg gtacaaaatt
    11581 ctttactttt acagacccat attatataat taagtaaata atgtatattt aggtgacata
    11641 ttggataagc taatttattt attttattaa tataatgata ctataaaagt tatataaagt
    11701 ggtttacaaa aaagttacag atagagcttt tattaatgta ttgtgtatta gtattttatc
    11761 cttaggtaca cttcacctac aaaatagaat cataagataa ttatgggaaa attttaggag
    11821 gtttatcgtg aagaaaaaga tattaatacc agtatttgca tcagttatgg cattatcagt
    11881 aagttcaatt gtaaatgcag atgaagtaaa tgattcatct caaaataagg atgataaaac
    11941 aaatactgaa ttaaatttag gtgaatataa agaagtaaaa tataaagttg caaaaataaa
    12001 agatggagta gctataaaga taagagaaga aggtcaagtt caaaacatag cttattctgg
    12061 agatgaattt acggttttag gaactcaagg agaatgggtt aaggttaaag ttgaagatgg
    12121 cgaaggttgg ttagctacta gatatgtaga tatatctgaa ggtgtaggat atactaacgc
    12181 tgataaagta aacttaagaa aagataagag tgaaagttca gaagtaattg aagagctgga
    12241 aaaaggaagt tctttattgg ttttggaaga taatggagat tggttaaaag ttaaagatgg
    12301 ggaaacagaa ggatatgtaa aatcatctta tatatctgat aaagctcctg taatagaaga
    12361 gccacaagta aacccaagta ttgaccaaaa tgtagattct acaaataata atcaacaaaa
    12421 caatgcaaat actaatcaac aaaataataa ttcaaatgtg ccaacagcta actctaatgc
    12481 agttcaagct gtacttaact tggcatattc taagcaaggt tgtccatatg tttggggagc
    12541 agaaggacca aacacttttg actgttctgg atttacacaa tatgtgtata gaaatgcagt
    12601 aggaaagaat ataccaagaa catctaaagc acaatctaaa tatggacaaa ctgtaagtaa
    12661 agcaaattta caaccaggag acttagtatt cttcacaact aatggttctg gttctgttag
    12721 tcatgttgga atatatgtag gtgggggtaa tatgatacat tcaccatcaa caggaaaaac
    12781 agttagtgta actagtataa attcatcata ctatacagca agatttgtta cagcaaaaag
    12841 aatattataa tcggtttgta atatgacaaa ttgataaata cataaatata tataaacgct
    12901 ctatattata tttaatttaa tcatggaaac aaaaaatgat tatattttaa atgtactata
    12961 gagcgttttt gtatcacttt aaatctaatc aagattcaag ttaatatgac tgttaaatag
    13021 gagccttttc atatgatgat tatttgcgca atcccttgac aggatagggc aaaggtggta
    13081 agctactaac agtacaaaaa tacttttaat tttagttcag agaggctaaa aaaggaggac
    13141 gaaagatgtt aaaatcaaga aatttaatcc agccagaaga cttttcaata gaagaaatag
    13201 atgaaatatt agaattagct caaaaaataa ttgacaatcc gtcaaaatat tctcgtatat
    13261 gtgaaggaaa actattagct acattatttt atgaaccatc aacaagaaca agattaagct
    13321 ttgaatctgc tatgaacaga ttaggaggac gtgtagtagg cttttcagag ccaaattcat
    13381 cctctgcatc aaaaggtgaa actctaggag atacaatgag gatagtatct ggttatgttg
    13441 atataatagc tatgagacat cctcaatcag gtgcagcttc agaagcagct agatatacgg
    13501 aagtgccatt tataaatgca ggagacggca aaaaccagca tccaactcaa acacttacag
    13561 acttacttac tataaaatca ttaaaaggaa ctttagaaag ccatacaata ggattatgtg
    13621 gtgatttaaa atatggaaga actgtgcatt ctttggttaa ggctatggca agatataaaa
    13681 atacaaagtt tgtatttata gcgcctgaag agttaaaaat gccagattat ataaaagaag
    13741 ctataaaggg acatgcatat tatgaaacta ataatttgga tgatgttata ggaagcttgg
    13801 atgttttata tatgactaga gttcaacaag aaagatttga agataaatca gagtatgaaa
    13861 gacttaaaaa ctattatatt ttaaataaag ctaaattaga aaaggcatct aaagatatgt
    13921 tagtaatgca tccattacca agagtaaatg aaatagacat agatgtagat agtgatgata
    13981 gagctgtata ttttaaacaa gctaaatatg gaatgtatgt tagaatggct ttaataataa
    14041 aacttttagg tataaatgaa gactaatagt tatcatataa tagtaaaaca aaggagattt
    14101 tgggatgtat aaaattctgg aaaatatata tataggcgaa gatatgtata gaatgaaagt
    14161 aaaaggtaat tttgaaggta aaatgggaca attctatatg ttaagagcat gggatactta
    14221 tccagtactt tcaagaccta taagtataca tgatatagat gaagaaggga taacttttct
    14281 ttataaagta gtaggagaag gaactcaaat tttgagtaac ctaaaggtaa atgataatat
    14341 aaaattagaa ggcccttatg gtaatggata tgctaaggtt gatggaaagg tagctctagt
    14401 aggaggagga atcggtgtag ctccactata tttggttgct aaaaatataa aaaattgtga
    14461 tgcatatctt gggtttagag aagatgttat attagaagat gaatataaac aagtatgtaa
    14521 taaagtctat acaacagtag gaaatacttt tgtaacagat ataattgatg ttgagaaata
    14581 tgattatata ttaacatgtg gtccaactcc aatgatggaa aaattagtta agatggtaga
    14641 aggtactaaa acgagaatta tggtatctct tgaaaaccat atggcttgtg gagtaggtgc
    14701 ctgtttagta tgtacgtgta aaactaatgg aggaaataaa aagacttgta aagatggtcc
    14761 agtgttctgg ggagaggacg tgatttttaa tgggtaattt aagtgtgaaa tttggaagtg
    14821 tagaatttaa aaatccagta ataatggcgt ctggaacttt tggatttgga aaagaatata
    14881 atgagattta tgatatacaa aaacttggtg gtataagtag caagggtctt actttaaata
    14941 aaaaacctgg taacaatggt atgagagttc atgagacatc atccggtatg atgaatagtg
    15001 ttgggcttga aaaccctgga gttcaaggat ttatagacta tgaattacct ttttttagta
    15061 aactagactt agtcagaata gctaatgttg gaggtgggac attagaagac tacttattag
    15121 gggttcaaat gttaaatgac aagcctatag acattattga attaaatata tcttgcccaa
    15181 atgtcaaagc tggtggaatg gcttttggta taaaaaatga agtggcaaga gaagttgttc
    15241 gtgaggtaag aaacataacg aaactgcctt tagtaataaa attatcaccc aatgcagaag
    15301 atattgtagg aatggcaaaa gtttgtgaag aagaaggtgc tgatggagtt tcattagtta
    15361 atacttttaa agcaatggca attgatataa aaaatagaag accagtattt gaaaatgtat
    15421 atgctgggtt atctggacca gctataaaac ctatagcttt gagaatggta catgaagtgt
    15481 gtaaaaatgt aaatatacct gtaatgggaa tgggcggaat aactaaagcc acagatgcta
    15541 tagagtttat aatggcagga gctacttgta tccaagttgg aactgcaaat tttatgaatc
    15601 ctagaatagg tattgaaata atagatggaa ttaatgaatt tatggataga gaaggaataa
    15661 aaagtttaga cgaaataaga ggaattatat aaggtagaat aaatagtatt ttaaagataa
    15721 aaaatataga ttaaataaac aataatttta taacattaac atggaggaaa aataatatga
    15781 gtaaagtaga tgtagtggat atattaaaga aaagtgatgc attgttggag ggacattttt
    15841 tattatcttc aggaaaacac agtaatagat atgtacaatg tgcaaaagta ttaagatttc
    15901 cagaatatgc tgctgaggta ttaagtacag ttgttgaaca aataaaagac ttagatatag
    15961 acttagtagt aggaccagct atgggtggag taatagtttc ttatgagtta ggaagacaat
    16021 taggaaaaga agctgtattt actgagagaa aagacaatac aatggagtta agaagaggat
    16081 ttgaagttaa aaaaggagca aagataataa ttgctgaaga cgttgtaact actggtaaat
    16141 caactatgga gacaaaaaga gtattagaag ccttaggtgg agaagttgta ggtgttgcat
    16201 gtatagcaga tagaactaat catgatatag gtatgcctat atatagtgct ataaaacttg
    16261 atattcaagt ttatgaatct gatgagtgtc ctttatgtaa ggaaggaaaa ttaccagttg
    16321 ttaagcctgg aagtagagag ttcaaggaat tagggatgta ataaaaactt aattatttat
    16381 agtgttactt aaaaaatgta aattttttag agtaaaaatt tgtaatataa catataactt
    16441 aaaaataaaa agcagttagc cttataaatg aaggttagct gtttttttgt taaaaatata
    16501 tttacaaaat aagagatagt agcagtacaa tataagtata aggtaaaagt attatatatt
    16561 agggaggggt aagtatgaaa aatcaattaa atgaaaatca taaaaaggtt gtattttttg
    16621 gagtaggtgc tgtaggagct acttttgcag aacaattttt taactctaaa tatgatttta
    16681 aaattctttg tgataatgaa agaaagaaaa gatatttaga agaaggattt ataataaatg
    16741 gaaaaaggta tgattttgat tatgtaacta aagatgagta taaacaagag gctgatttta
    16801 taattatagg tctaaaatat aataatttaa aagaaaatat aaaagaatta gatggattag
    16861 ttggaaaaaa tacagttata atgtctctgc taaatggagt tgatagcgaa gagataatag
    16921 gagaaagatt tggaattgaa aaaatggtat attcatatgt taccaatata gatgcaaaga
    16981 aaatcaataa taatattata cacactacta atgggataat tgtatttggt aacaaagata
    17041 atagtgaaga tagaaaaact aatataataa ccgaagtctt tgatgatgta aatatagaat
    17101 atactttatc aaaagatatt caacgagata tgtggtggaa gtacatggtt aatattggtg
    17161 taaatcaaac ttcagctata cttggtgcac cttatggagt ttttcagagt tctgagcatt
    17221 taagagaatt agcaaaatct gcaatgaggg aagttgttgc tatagcacaa gcaaaagaca
    17281 tatctcttac agaagatgat gtggaacatt cattacatag aatactagaa cattcaaaag
    17341 aaggaagaac atcaatgctt caagatgtgg aagctcatag acttacagaa gtagatatgt
    17401 tttctaagaa tatctgcaaa cttggaaaaa aatataacat acctactcct ataaatcaga
    17461 ctttctttta tatgataaaa gtaattgaaa gtagatttta aagggtcttt gtaagaaata
    17521 ttgataatta ttgattttaa attttattaa tgttatatac tatttaagtt actaaatatt
    17581 tttaactctt caatgaaaat agttgctgct tttgataaag ttatatcttt tggaaatata
    17641 tatgagaaat gtctgttgta gctgcttcca agctctatga cagacagctc attgtttaga
    17701 acaggtaagc ttgtcactag attagagact attgtaattc ctaaattatt tctaacagat
    17761 tctttgacag cataattact tccaaaaacc atcatacttt ttggaataat ttctttaaca
    17821 cttaaaaaca tatctaaata atctctggtt ccagaaccat cctccctgac tatccatttt
    17881 tggttttgaa gtttatcaaa actaaaatct tttaataaat gactcttgta tggaagtgct
    17941 aaaaccatct tatcttcaaa aaaatattct tgaataaatg aaggggaaga gcaagtgcct
    18001 tcaataagac caatgtctaa aatataatcc ttaacatgag aagatacgat tgatgtattt
    18061 tttataaaaa cttctacatc tatgtcagga tactttttag aaaataatgc tagaaaattt
    18121 ggtaaaatat actctccaat agttaaactt gcaccaatct ttaagtgacc agttatagca
    18181 tctgaaactt ggcttacatc atgataagta atatttaaaa gatttaatat ttctttagct
    18241 cttttgtaca agatttgtcc actttctgtt ataaaaatgg ttttttgttt aatagacctg
    18301 tttataagca caactttaaa ataattttct agatttttta tgtgtttact tacacttggt
    18361 tgtgatagat ttaaatattc cccagcttta gtaaagtttt tgtattcaac aacagctaca
    18421 aatgttttta attcttcaaa caattttttg tctcctttac ctaatgaaat tttacatttt
    18481 ttatatactt tctacagata attatactat atataataac cattaaaaac attaatcatt
    18541 acgatattat atatttattt tacttatggt tattacaatt atatactatt gctatgaaga
    18601 aaactatatt aattataagg taggtatatt atgttaaaga aaaaaagcag tttgacatta
    18661 aaaactataa gggaaatatt accaggactg tttgtatcag tattagtagg ttatattagt
    18721 atatttattt ctattttaat tcctaaggta ggggcagcat caatttctat atttttaggt
    18781 atgtttgtag gaaacctatt tttaaatcaa aaggtatttc aaaaaggtta taaattttca
    18841 gagacagatt tgttatcata ttcaatagta ctattaggag caacactcag tgtatctacc
    18901 ttaatagact taaaagtttc aggaatttta tttataatat tgcaaatgac aataacaata
    18961 atagctgcac tttatatagg taaaaaacta ggatttgaag aaaactttag atttctaatg
    19021 gcaagtggaa atgcagtatg tggttcttct gcaataggag caacagttcc agtggttaat
    19081 gcgacagata aagaaaaggg aattgcagta actattgtaa atgttactgg tatatttctt
    19141 atgtttttac ttccaataat atctcaattt ttatatagcc atgaacttgt aaaaacttca
    19201 gcaatgatag gtggaacttt gcaatcaatt ggtcaggttg tagcaagtgg agcaattgtt
    19261 ggagaaaatg taaaagattt ggctactatt tttaagatag taagagtaat atttctagtt
    19321 gttgttgtat tagtatttgg acatatgaaa aatcaatcta atagggaaat tcttgaagaa
    19381 gaaaaggatg agataaaaaa gaaaaaagtg actataccat ggtatgtaat tggattcttt
    19441 ataacttgtg cacttttttc aatgaacatt ataccaaaag aagtatctgt gttgtgcaaa
    19501 gaaataagta acaaactaga aataatagct ttagcagcaa ttggattaaa ggttaatgta
    19561 aaagatttag tgaaacaagg gaaagaagtt tctttatatg gtttatttgt aggtatagta
    19621 caagttgtat cagctgtagt tttaataaaa atatttatat agcataaaaa tagtatttgt
    19681 aatattaatt tgattaacta aaataatatg atactaataa tttataatat aaataaaaaa
    19741 tgaatttcga taagtttaag tgggtataaa cttatagaaa ttcatttttt atttaaggta
    19801 aaaatttaaa gtaaaatatt attttacata ataataacct tataaatttt gaaattactt
    19861 atctttgtgt taaaataagt aaaatgcaca tattagagct aagtttttaa gtaaataaac
    19921 aaaatgaaaa tataaatatt aaggagattt ggtatatgaa tgtttatgaa aaaggtaatg
    19981 gagttatact agaaggagta acagattttg acccagtaca tatatttgag tgtggtcaat
    20041 gttttagatg gcacaaacaa gaagatggct catacactgg agttgcaaag ggaagaatat
    20101 taaatgtcaa aaaagagaat gataaaatat atttaaataa tactaattta aaggagttta
    20161 acagcatttg gtataattac tttgatttag gaactgatta tacagaaata aagaataagc
    20221 taaaaaatat ggatgaatat ttaaataagg ctactgaatt tggatgggga ataagaatat
    20281 taagacaaga tggatgggaa atgcttatat catttataat ttcctctaat aataggattc
    20341 ctatgataca aagagcgata gaaaatttat caagaaagtt tggtaagtat ataggtgaat
    20401 atgaaggtaa tgaatactat gcattcccaa caccagaaga actaaataag gcctcacaag
    20461 aggaaataag agcatgtcaa acaggtttta gagataagta cataaaaagt actacacagg
    20521 cagttataga gaataatgat gaggtatctg aatatacaaa tttaagtaca gaggattgta
    20581 gaaaagagct tttaaaattt aatggagtag gaccaaaagt atgtgattgt attgcattat
    20641 ttggtatgca aaaatatgat tcttttccag ttgatgtttg ggttaagaga gttatgcaag
    20701 aattttatat agatgaagac atgagtttac ctaaaatgag aacttatgga atcgataaat
    20761 ttaaagaaat gtctggattt gctcaacagt acttatttta ctatgcaaga gaactcggta
    20821 taggaaaata aattaagggg gagaaattat gggagtaata ggaacaatat ttctgttcta
    20881 tttaattata tcatatctag ctggagccat aatttctgtg attatattgc ttgaaaatag
    20941 agaccctgca aaaacaatgt cttggctatt gatgtttatt atatttccag gtgtaggttt
    21001 aatgatatat gctatttcag gtagaaatat tagaaaaaga aaattattta agactcaaaa
    21061 actagctaat aatataaaag aaaagaaact atttgataca ttagaaaaga taacagaaat
    21121 agttgaatta gaaaaagaat ctataaaaca aaataagctt ttaagagatg aagaggatgg
    21181 tagttataga aaaagggtaa taaatatgtt gttaaagact ggtatgtttc cctttactaa
    21241 aaataataaa gtggatgtat ttgtagatgg aaatgaaaag tttaaaagac ttatagaaga
    21301 tataagagaa gctaaagacc atattcattt agagtacttt atcatcaaag atagtgaaat
    21361 aggtagggtg ttaaaagaag aacttataaa aaaggctaaa gaaggcatta agataagaat
    21421 tttatatgat gatgtaggat gctggagatt ttggtttaat agaaaatttt ttcgagaaat
    21481 gagggaagta ggtatagaaa ttgcagcatt tttacctaca aaatttccta taataggagg
    21541 gaaattaaac tatagaaatc acagaaaaat tgttgttatt gatggaataa ttggatatac
    21601 aggtggaata aatataggag atgaatattt aggcaaaaat gataagtttg gatattggag
    21661 agatactcat attagaatta agggtatttc tgtatacatg cttcaaatga catttctgat
    21721 tgattggtat tatacgacaa aagaagtatt agtgactaaa aattatttcc caagtgtagg
    21781 gaatgttggt gaaagtatga tacaagttgt tgcgagtggt ccagacagtg actgggaaga
    21841 tattcattat gcttattttt ctgcaatatg tcaggctaga aaaaatgttt atatagaaac
    21901 tccatatttt attccagatg aaagtctact aaaagctata aagagtgctg ctcttagtgg
    21961 agttgatgtg agaataatat ttcctaaaat tgcagaccac aagatagtaa atatagcatc
    22021 ttattcatat tttgaagaaa tattaagagc aggtggtaaa gtttatttat ataataaagg
    22081 atttatacat tctaaagtcg taataataga tgataaaatt gcttctgcag gtacagctaa
    22141 tatggattta agaagtttta tgcttaactt tgaggtaaat gcatttatct atgatgaaga
    22201 agttattagg gtaatgacag atgatttctt tgaggattta agtcactgtg aagaacttaa
    22261 tttggaggta tttaagaata gaaatatcat acaaaaaatc aaagaatctg tagcaagatt
    22321 attttcacca atattataaa aaattattaa aaaaggtatt gaaaattatg tgtgattggt
    22381 atattataat aattgttagc actctattag atagagtgat aataaataaa aagtatatat
    22441 ataaatctac atatataagt agtattaatt taaaaataac taaaaacata ggcgaactag
    22501 tctatactta atattttagg aggcgtgaat aatgaaaata agaccattag ctgacagagt
    22561 agtaattaaa aaagtagaag cagaagaaaa aactgcaagt ggaatagttt taccaggagc
    22621 agctaaagag caacctcaaa tagctgaagt tgtagaagtt ggaccaggtg gaatagttga
    22681 agggaaagaa ataaaaatgg aattgacagt aggagataag gttatattcc aaaaatattc
    22741 tggaacagaa gttaagatag aaggacaaga atacacaata ctaagacaga gtgatgtatt
    22801 agctgttatt gaataaatat agaataaatt tattaggagg ggtttaaaat ggctaaagaa
    22861 attaaatttt cagaggaaac aagaagagct ttagaagctg gtgtaaataa attagcagat
    22921 acagtaaaag taacattagg acctaaagga agaaatgtta tattagataa aaaatttggt
    22981 tctccactta taactaatga tggagtaact atagcaaaag agatagaact agaagataga
    23041 tttgaaaata tgggtgctca attagttaaa gaagtagcaa ctaaaactaa tgatgtagct
    23101 ggagatggta ctacaactgc tacagtttta gcacaagcta taataagaga aggtttaaag
    23161 aatgtaacag caggggctaa cccaatactt ttaagaaaag gaatacaaaa agcagtaaca
    23221 gtagcagtag aagaattaaa aaatcaatca agaatagtag aaacacaaga ggctatatct
    23281 caagttgctt ctatatctgc tggtgatgaa gaagttggaa aattaatagc agaagctatg
    23341 gaaatagtag gtaaagatgg agttataact gttgaagagt ctcaaactat gaatactgaa
    23401 ttagatgctg ttgaaggtat gcagtttgat agaggatttg tttctgcata tatggttaca
    23461 gatgtagata aaatggaagc agttttaaac gacccatata tattaattac tgataaaaaa
    23521 atatctaaca tacaagaatt attaccagtt cttgaacaaa tagttcaaca aggtaaaaag
    23581 ttattgataa tagctgaaga tgtagaaggt gaagcattat ctacattagt agtaaataaa
    23641 ttaagaggaa catttgatgt agtagcagtt aaagctccag gctttggaga tagaagaaaa
    23701 gaaatgcttc aagatatagc aatacttaca ggtgctcaag taatatcaga agaattaggt
    23761 tatgatttaa aagaagctga tttatctatg ctaggtagag cttcatctgt taaggtaact
    23821 aaagaaagta ctacaatagt agatggttct ggagataaaa aagctataga agatagagta
    23881 actcaaatta aacatcaagt agagcaaact acttcagatt ttgatagaga gaaattaatg
    23941 gaaagattag ctaaacttgc tggaggagta gctgttgtaa aagttggagc tgctacagaa
    24001 gtagagttaa aagaaagaaa gttaagaata gaagatgctc ttaatgcaac tagagcagct
    24061 gtagaagaag gaatagtagc tggtggagga actgcttttg ttagtgttat accagcaata
    24121 ggaacactaa ttgagagttt agaaggagaa gttaaactag gtgctcaaat agttaaaaaa
    24181 gcgttagaag agccattaag acaaatagct ataaatgctg gtcttgaagg tgctgtaatt
    24241 gtgcaaaatg ttgttaattc tgaagcagaa actggatttg acgcattgaa tgaaaaatat
    24301 gtaaatatga tagaagctgg tatagttgac ccaactaaag ttagtagaag tgctttacaa
    24361 aatgctgcat caatagcaag tactttctta actactgaat ctgctgttgc tgaccttcct
    24421 gaaaaagaag atgcaggaat gccaggaatg ggtggcggaa tgcctggtat gatgtaattt
    24481 aaaaaattta aaatataaat tttaatataa agaactattt cgacttagat tttaaactct
    24541 aagttgaaat agttcttttg tatatatcta atttttgtat atctataaat tcaatacaca
    24601 tatttagata atcagtatct agtttgtaat aatattttag agtagtattg ttatttttta
    24661 ggttatgatt ttagatatat attaattaag gtataattgt tgatagagaa taaatcaaat
    24721 ttaaaaggat gcgataatat ggacttaaga aaattgcttg aagaagttaa aaatgaaaat
    24781 attgatatag attttgcgtt agaaaagtta aaagatttac cttatgaaga ccttggatat
    24841 gcaaatatag accatcatag agaattaaga aatgggtatc cggaagttat ttattgtgaa
    24901 ggtaagagtg atgagcatat tttagggatt attgataaaa tgaatcaaaa aagttctaac
    24961 atattgggga caaggtgcag aaaagaaact tttctgaaaa ttaaagaaat ctataatcat
    25021 gctgagtacg aagaggcgtc taaaattctt aagattcaaa atcatgatat agagaatata
    25081 ggaaaaggta aaattgtgat tgctacaggg ggaacttctg atataccagt agcagacgaa
    25141 gcttatcata cagctaagtt tttaggtaat gatgttgata gaatatatga tgttggagtg
    25201 gcaggtatac ataggctttt aaataaaaga cataaaatag atagtgctag agtaatagta
    25261 gccgttgcag gtatggaagg tgctcttgca agtgtaattg gtggactggt tgatgttcca
    25321 gtaatagctg ttccaacttc tgttggatat ggagcaaatt ttggaggatt agcagcattg
    25381 ctagctatgt taaatagctg tgcatctgga atatctgttg taaatattga taatggattt
    25441 ggagcaggat atttagcatc aatgataaat aaactgtaaa tacattgata taaattaaac
    25501 aaaactatat aataaaattt aatttctatt atatttttac tattaaaatt gaggagacat
    25561 atatgagtga aaaaatactt tattttgata taataagcgg tatatcaggg gatatgaccc
    25621 ttgcaagttt attaaattta ggtgtgccta aggaaatttt tttggaggaa attcataaac
    25681 taagaatgag cgatgaattt aatataaaca tcagctcgaa aactgaaaat ggaatagtag
    25741 gtacaaaagt agaagtaatt acaaaagaaa aacatacaca tagaaattta gtggatatat
    25801 ttgaggttat agataaaagc actctaaacg aaaatgtaaa atctaaagca aaaaaaatat
    25861 ttatggtaat tggagaagca gaagcaaaag ttcatggaac tacaatagac aagatacatt
    25921 ttcatgaggt aggggctata gattcaattg tagatatagt aggagcttct atattagttg
    25981 acttactttg tgtagataaa gtttgtgcta gtacagtgtc tgttggttca ggatttgtaa
    26041 aatgtgaaca tggaattata cctatacctg cacctgcaac tatagaaatt ttaaaaggtg
    26101 tgccaattaa attaaataat gtaaatggag aatgtacaac tccaacagga gctgcaatca
    26161 taaaagtttt gtgtgatgag tttgtagatg agcttgaatt ttctccaaag caaattggat
    26221 atggtatagg tcataagaag tttgaagtac caaatatact cagaactgta ttaggtgaaa
    26281 aaaaaaagaa gaattaattt atgaaattag tgcaaacatt gatgatatgt cttcagaaat
    26341 ctattcttat ttgtatgaaa aaatattaga tgagaaagca ttggatattt atacagagag
    26401 catttatatg aagaagaata gaccagcaac aaaagtatca attttatgta gtgaatcaga
    26461 tttagaaaaa tttattaaaa tattactttt agaaactagt acttttggag tgagatataa
    26521 tgcatataat agagaaattc tagagagaaa atttgtaaaa ttagatacaa aatatggagt
    26581 ggttacagta aaactagggt attataaagg aaaactagtc aaggctactc cagaatataa
    26641 tgaatgtaaa actatagcaa aaaatgaaga tattcctata aaaaaagtgt ttgctgatat
    26701 aaattttaga ataaaagaag aatttagtga aaacttattg acataagtaa taaattttga
    26761 taaattaata ttcagcctta ttaaataaaa gtaaacagaa aaaaatatat aaaatataaa
    26821 ctcactcgta tatgctcaga aatatggtct gagagtctct accaagatac cgtaaatatc
    26881 ttgactatga gtgaaattat tataccaaaa gacctagtta agtaaatgtg taggctgagt
    26941 taaactccct atgtgttggc tataagataa aggtattttg gaagcctgta tagtaatttc
    27001 acttttgcta gaaattatgg aagttactat acaggttttt tgtgtaaaaa gatttaatat
    27061 aatttagact gtattgtgtt aaaataaata tataaaaact atattataca aggagagatg
    27121 tgaatttatg aaacatgaat tagtacttgt aatagatttt ggaggacaat ataatcagtt
    27181 gatagctaga agagttagag aaaacaacgt atattgtgaa atacttccat gtactgcaag
    27241 catagaaaga ataaaagaaa aaaatcctaa agggataata tttacaggcg gtccaaatag
    27301 tgcgtattta gaagattctc caacaatatc aaaagaaata tttgaattag gagtaccaat
    27361 acttggaata tgctatggta ttcaaataat gtctcatgta ttgggtggag ttgtaagaaa
    27421 aggtaataaa caagaaaaag aatatggaaa aacagctata acttatggaa aatcatcatt
    27481 atttgaagga ataactacaa atagtgtatg gatgagtcat acagatttaa tagaaaaagt
    27541 tccagaagga tttactatag ttgcaaatac taatgattgt ccagtagcag ctatggaaaa
    27601 tgtagaacga aacctatatg gagttcaatt ccatccagag gtggaacact gtttggaagg
    27661 ggataagata cttacaaact tcttatataa tatttgtaaa gtaaaaggtg actggactac
    27721 agattcattc atagaagata aaataaaaga attaaaagaa aaaataggcg ataaaaaagc
    27781 tttatgtgct ttaagtggag gagttgattc ttctgttgct gcagttctta ttcataaagc
    27841 aattggggac aacttaactt gtatatttgt tgaccatggt ttacttagaa aaaatgaagg
    27901 aaatgatgta gaaagaatat ttagagagaa atttgatata aatttaataa gagttaatgc
    27961 agaagataga ttcttatcta aattaaaagg agtttctgaa ccagaagcta agagaaaaat
    28021 aataggtgaa gaatttataa gagtatttga agaagaatca aataaattag gaaaaatgga
    28081 tttcttagta caaggaacaa tctatccaga tgttatagaa agtggacatg gaaatgctgc
    28141 tactataaag tctcatcata atgttggtgg aatcccagaa gatgtagact tccaagaaat
    28201 tgtagaacca ttaagagagt tatttaagga tgaagttaga aaaataggat tagagcttgg
    28261 tatagaagaa ggattaatat ttagacatcc tttcccagga ccaggtctag gaataagagt
    28321 tataggtgat gtaactaaag aaaaatgtga tatattaaga gaagctgatg ctgtttatat
    28381 ggacgagctt agaaaagcag gtttatacag agaaatatgg caagcttttg caacattacc
    28441 agatgttaag actgttggag ttatgggtga tgaaagaaca tatgcttatt tagttggttt
    28501 aagagcagtt acttcttctg atggaatgac ttctgactgg tataagatgc cttatgatgt
    28561 attagaaaga atttctaata gaataataaa tgaagttgat ggagtaaata gagtagttta
    28621 tgatataact tctaaacctc ctggaactat tgaatgggaa taaaatatag aatgccaaaa
    28681 atccacataa atacaggatt taaggtctct cttacttcta tgttatactg gtttgatact
    28741 atcaatattt gccagtatct tatggattaa taagtaaaaa acatcctgct gaatgataaa
    28801 ttcagcgggg tgtttttatt ttgttttaga actcctttgc tctgaaactc gaagtattta
    28861 acactgatga ttttgttatc ttttagttcc ttttgtgtca tgctctgaaa gacaaattga
    28921 tacaataaaa aagtaatttc ttaatcattg atggagatat gtatttatgt tactcttgat
    28981 ggtaatttga gtaatattgt tcatagcttt ttatattcaa ttaattagaa aaccatttgg
    29041 tataatcata aataaacttg atgatataat agaaaagatt tataattatt agaagtatat
    29101 aaaagtattt tatgtctatg aaatgtatgt ctataaaaca tatagatgac ttttgtatat
    29161 taatgtatga atattgatat tatagaagaa ttgagttgtc aatgatacta aatcaaatat
    29221 ttatagaagc tggagggatt atgagaaata aaataattat aggaattact tttttacttg
    29281 tattactaat gtctggatgt gcttcaaaac aaaccgcaga aataaacgta ttagcaacta
    29341 ctgatttaca tggagttata ccttatgagt taacttctta tgtaaaagaa gaaagaaaaa
    29401 aagataaaaa tctaacttta gtagatgcag gagatttttt tgatagtgga gaagtgtttg
    29461 gttctagcat ggatagttat tttagtgcta gatataaaga tggtgataat aacactgaaa
    29521 gttatattga gacgcctatt tcaaaagata tgaaagaggt aggttatgat gttgcagttc
    29581 ttgggaatca tgaatttata tccaacaata aattttatct agataatatg atatctgatt
    29641 ttgagaagca aaaaataaat attttatcgg caaatacgta taaaaaaaat ggagacagtt
    29701 atacaaaacc atacataata aaaaatatta atacacctga aggaaacgta aaactaggaa
    29761 ttttagggct tactataaaa gaagttggtg agaggaaaca gtggaaagat ggaaaacttg
    29821 ttgactctaa gtctttagaa ctaaaagacc aagagggcta caatggtgaa ttgtatatga
    29881 atgatttagt agaagatgct aaaaagtggg ttagtataat ggaaaaagag aagcctgaca
    29941 ttatagttgc tgtggcacat acaggtgaga aacctaagaa acctaaaaac ccaggaaata
    30001 gaatacaaga cttagctcaa caagttgatg gaattgatgc tatagttgca ggtcataatc
    30061 atgttcaaat tgagcaacat gattataaaa ataaatcagg agaaaatgtt atagttactg
    30121 agccaggaaa gcatggggaa tgtatttcta agattaattt taaattagaa aaaaacaatg
    30181 ataaatggaa tatagttgac aaatctagta aattaattaa atttgaaaaa aatcaagaaa
    30241 gtgataaatt tggagaattt atttcttcaa ttaataaagt aagtaaagat aagaaagaaa
    30301 taagattatc agaagtaaag actcctgaat gggataaaat atatgttttt aaatctggaa
    30361 catctgcaga tgatatatat aaaacaatag gatataaatg gagaaatata tcatctccaa
    30421 agagtgatgg catggtgcag attgttgtaa tgaataataa taagcctgtt agctatatat
    30481 atggtgaagc tgaagttatg ccaattaata taaaatgtga tgaatcagag tataaagata
    30541 atgttgtaac tatttatcct aataaaaatg ataaatttaa aattgaaaaa ggtaaacaag
    30601 aatatagtat taatttaaaa catatacaat agaaactttt atcttattta agtttagatt
    30661 gtacttaaaa taaagtgtgt attattttaa aaattgatga taattaaaat cacaacatta
    30721 aggaatagta cacacttttt atgctaaaaa atagaatgtt aaaaaattaa acctttaaat
    30781 taaagtacat tgcttatatt atccaatatt atcttcaaag gttgtgtaat atttttatcc
    30841 ttatgtaata ttgctaacat tttattttcc atatctatcc catctaattc aatcaatcgc
    30901 aattttttat gtaagagtga atctaataat aaattttctg gcaaaatggt tatacctaac
    30961 cctgcttgtg cagctttaat aagtgcaaaa gaattaacac tttcccaaac aggataagct
    31021 ttttggtttg aaagagataa tacactgtcc aaagtatcgc gaatggcact tccttgctca
    31081 cgtaacaata acggaaacgt gcataactgt cgtagagata aggcttgttt agtattggtg
    31141 gtggttatgc aatcatacct ttaatccaag agaaagtagt aaatcaaact ggatggataa
    31201 gtgagaaaat atttacagat attataacta tttcacaaat gacaccaggg ccactggctg
    31261 ttaatacttc tacttttgtt ggacttcaaa ttggagggtt ttggggagca gttgcagcca
    31321 ctttaggttg tgtattatgt ggtgtagtaa tatctctatt tttatataat ttttttcaaa
    31381 agcatcagaa ctctacttat atatttgaaa tattaaatgg tctaaaatca gcttcattag
    31441 gtttaattat ctcagctgcg gctacaatta taattcttgc attatttggg acgaataaag
    31501 tgtacttcaa ttctatattt gagatgctaa actggactgc actaataatc ttttttgtta
    31561 cactactgat agtacgtaag tggaaaatta atcctattgt gattataata atgagtggta
    31621 tactgggaat atttttttat ttataagttt ttgctaagta aaggatatct atttttttat
    31681 atcttaaatc caaatagcca ataaagtaag gctatttgga tttttaataa attctgaaaa
    31741 gataatcaga aacctatata acatttaaat tatatcactg gttttctaca aaccaatcat
    31801 aagcttcttg gcaaaatttc ttactatcta tatagtaact attatgaaca ttattaattt
    31861 caacaagttt ctttttacat tttagtgaat tataatagct atcaaaatat tgggttttaa
    31921 aatatctatc atgaagtgtt ttaaagacaa gagtaggtat gttgactcca tcttttatct
    31981 tactatcttt tgcaataaag cattctaaca ttgtattaat tatctttgta ctatttttcc
    32041 aaatatgagg atttttcttt tcaaatattt tataaaattc attatcatag acttcattat
    32101 aacctttata tttaggtggt tttactgtta tgtgaaaatt aggaagaaac ttaaagaaaa
    32161 aatataataa tttttatgtt ctgttttata tattattcat attttatcac agataatata
    32221 ctaaagttaa tttttttatt gtacaactat tataataatt cttctataat ataagacatt
    32281 ctataatttt atatttaagt tcatgtgtat ttaattgttt ttatataaaa tgtccagtaa
    32341 tactatttgg atttattttt atttcatcag gagttccaga agcaacaatt tcccctccat
    32401 ttattccact gtctggacct atatcgataa tccaatcaga agcacgtata acctctgtat
    32461 tgtgttcaat aattaaaagt gaatgctgtt catttacaag ttttctaaaa gtaattaaca
    32521 aattttctat atctttaaaa tgaagtccag tagtaggctc atccaatatg tacagcattc
    32581 gttttttagt ttttgtccct aattcttttg ctaattttat cctttgagat tctcctccac
    32641 ttaatcctaa tgtattttgt ccaagcttta gatatccaag tcccacttca tctaataagg
    32701 agagttttct atatatatca gagcaatcgc taaaaaatgt ttttgcttct gctacttcca
    32761 tgtcaagtat atctgcaatt gtctttccct tgtattgtat agacagtatt tctttttgat
    32821 atctcttacc ttgacattca ctacaagtta cccattggtc tgccatatat tgcagaataa
    32881 tttttgtttt acctaatcct tgacagtttg ggcattgccc ctttttgcta ttaaagctaa
    32941 aatagctttc atccaataat ttttgttttg cttgtggagt ttcagcaaat agagaacgaa
    33001 tttcatcaaa aatattgata tatgtagcag gtgtagaacg gctagatttt ccaataggtg
    33061 cttggtccat taagataaaa tcatcaatag catcaaatcc agtaaattca gtataatttt
    33121 tatctgggat agatttttgt ttcaattttt cttctagagc aggaagtaaa ctgtgaaata
    33181 caagagagct cttaccagaa ccactaacac ctgtaataga gcacatacag tttaagggaa
    33241 tttctacatc aatatttttt aagttatttg catggcatcc ttttaagctt aaccatttac
    33301 ttggtataat tttattttgt gaacttattt tattgtatgt acttaaatat ttacctgtta
    33361 tagagtttgg attttttata atttcttggg ttgtaccttc tgcgataatg aacccccctt
    33421 ttgtaccagc acttgggcct acatcaataa tataatcagc agacagtatt gtatctttat
    33481 cgtgttccac aactataaca gtattaccct tatctcttag ctcttctatc attttgatga
    33541 ttaaattatg gtctcttggg tgtaaaccaa tagatggttc atctaaaatg taggttaagc
    33601 ctactaattc acttcctagc tgacttgata atcgtactct ctgcagttct cctccagata
    33661 aagttggagc tgttctatct gttgatatat agctaagacc tacctttaat agataactaa
    33721 ctcgaatttt taattctgtt aagatatcat ttaccaaaga gagctcattt ttttgaaggt
    33781 gatttggtaa ttcacacaac caattccata actgttctat cgtcattttt gttagttcag
    33841 ggaagcgata acctttgatt gtagtgaatc tagcctcaat gcacagcaat tcgccaccac
    33901 atgtattgca aggaattttg ttcatatatt gatgcaaggt attattttca gaactagatt
    33961 ctctaaaaag tctttgaatg tggttgattg ctcctgaagc aggtcttcta atctttgatt
    34021 ctcgtccctt tgattcaaag ctaaattcat atatcttatc gtctgtacca tataagattg
    34081 catctataaa atctcttggt aattcattcc aaggtatatc taggtctata tttttatctt
    34141 ttgctattgc atataattca cctaccatcc aattaccatt tggctttttc cccctcaatt
    34201 ttccaaaata gtaagtagct ccatctaata ttgaaatagt tgggtctttt acaattaatt
    34261 gaggattaac ttgataggta aaacctaatc cattacaatc gtgacaagct ccagtatggg
    34321 agttagcatt gaaggttgaa gtatttatct tagaaaaaat agtttgacaa tgtggacaat
    34381 aattttttgt attattcttt ttttctaatg gaattttgca atatggacag taactttttc
    34441 cgattgatac aaataacatt cttaaataat cccaaatgct tgtaagagta ccaacagtag
    34501 agcgaggatt ttggcttcct tttttctgtt cgatggctat agtaggggtt aatccctgaa
    34561 gtaaatcaaa ttccggtttt tgaagctgtt ctgaaagttt tgctccgtta cttagatttt
    34621 caaaataacg tctttttcct tcttcataaa tcgtatcgaa agctaaagaa gattttccac
    34681 taccacttac acctgtaata acagttattt gattttttgg aattgtaact tcaacatcct
    34741 ttaaattgtt ttgccttcct ccttttaaat agatagagct tttattatga caaagtgaat
    34801 ttttaattga tttatttatt gcttttgtaa atttgaattt cttttctagt tcaagaggat
    34861 tgcctaattc ttttatttcc gaaataccta atatttttaa ctgagaaaag attgatttca
    34921 aataatcttc aggcaatttt tctattaaca aattaagggc ttctattttt atatttccgc
    34981 ttgataatat ctctgccatt tcatctaata aatttttttc tgctcttgtt aattgattgt
    35041 attttttgga acttaattct gtccataaat cccatggaag caattctaaa ccaatcatat
    35101 tattaaagtc atgtagaaga ttacccttta cagaatgcca acctttccaa tttttattat
    35161 atccaaagtc atctggatga gcatgtcctt gccgacatct tatccaggct tctgaaccag
    35221 ttaaaaaacc atctgttttt ccgacatcat gagtgtcaaa attaatttga aaataatttt
    35281 tttgtatgtc atctatttgt gcatctattc ttatccatct ctttgttaga gtatcatagt
    35341 attctacaag ccagtggtct tcataagtaa gctcactttc aaaataatta gcaaaacctg
    35401 ctctcattct agcctcaaaa cctcgatatc ttaggagcga tacaagaagt aaagaataat
    35461 ctctgcacat tccaacaagc ctttgttttg ggtttcttgg aatagttagt gggttagaat
    35521 ttatttcaaa tattcttttt aacatttgag gaattgtacg aagcaattcc tcatcagatt
    35581 gttgcttatt aaaggatata ccatataatt ttccttggtc tccatgaatt actaggcttt
    35641 gaaccacttt taataactca attggagtct ttggtaaatc ttgatattta ttttcaaatt
    35701 ttttaggatt tgtgatgata ctttgatttt tatagttatt tattttcatt tatatttcac
    35761 ctctctaaat ggatgatata ccctgacatg gtgtcatatg caaggacaat ttagattttt
    35821 ggccaaaatt taaaaaataa ataaaaaatg atacagtgat aaattgtaat acaaatatag
    35881 tttcaaattg catataatat aattatttaa taaaaccatt atctattgaa tgttaatgaa
    35941 atgttaagat ggcattcttg aatattaaga aatagaatac tataatatag tgtgaaggat
    36001 ttttagtaaa aaaatttgta agtgttaata tacattaaaa aataggttta taatttggag
    36061 gaaaaaaatg aaattatcaa ggtataagaa aaatagttta gttctgtcta taattttatg
    36121 ttcacttatt atttcaatcc ttgtaattac tgtttttaat accgtaaaac tacacaatat
    36181 atctaataaa caatctaaaa actatttaaa tgatgtatct acacagattg taatgaatgt
    36241 tgattctaaa atcaaattta ttttatccga cttaagaata atggcagatt ttattaagca
    36301 atatgaaggt gatagtcgat atgagtattt atctaaaaga aagactagtt ataagtatta
    36361 tgatgttgga ataatagatt tagaaggtaa agctgagttt ttaagtggaa aaaagtttga
    36421 cttaaagaat actgtttcct ataaaaaagg aattgaaggt aaagaatact cagaagtaat
    36481 agagtcaatg ggttttgttc tttatagtgt acctatattt aatgataatc atgaagtatc
    36541 tagtatttta ataggtgtat ccaataaaga aagtatgaat gagattctta atatagataa
    36601 ctttaatgga aaaggaacta ttgaaattat taatagtaag ggaaaacctc ttttcatagg
    36661 aaaaaatact gaattaataa aggaccttag ccataaatat aagagttctg gagatgaacc
    36721 ttgggcacaa aagatgcttg aggattttaa aaatgataaa tcagggaata ttacaataac
    36781 ctcatcaaaa ggtgtacaat gcttattaac ttatcatcca attacaagag gtttaaatga
    36841 ttggcatttt cttctcatag tacctgaaga tgctgtactt ggtgaactta ataaattaaa
    36901 ctcttttaca ataacaatga catttataat tacatgtatt attggaatta taacattaat
    36961 tttatatatt ataagaagaa aatatgtaaa tcaaatagaa aatattgcat atttagatag
    37021 tataacaaat ggtataaatt caactaagtt tagtatgcta gtcaaacctt taatttcaga
    37081 atcacctgat agtacttata tgatgatagc aatgaatata aaagatttta agctaataaa
    37141 tgattgtttc ggtagtgaaa agggaaatat gacactaaaa catctttaca atattttgta
    37201 taaaaacatt gatgaaaatg aagaatttgt gtgccgtcat gatgcagacc ttttctattt
    37261 gcttattaag aatagaccag tgttagaagc cctggagtta ttatataaaa tagaagctga
    37321 cacaaattat tttaataaaa atagggaaaa tccatacttt ttaagactat cagtaggtat
    37381 ttacacaatt gaaaatcata atgaagattt aattacaata caagaccatg caaatacagc
    37441 aagaaaaagc tttaataaaa ctcatcaaag tgatttttct ttttatagtg atatagaaag
    37501 aaaacgatta atatctgaaa aggaaatttc aaatttaatg gaaaaagccc ttgaaaataa
    37561 agaatttttt atgtgcttac aacctaaaat tgatataaaa agtggaaaaa ttagtggagc
    37621 agagtctctt gtgcgttgga aaaattcaga aaagggcata atttatccaa gtgattttat
    37681 tcctcttttc gaaaaaagtg gatttatatg taagttagat ttatatatac tagaagaaac
    37741 atgcaaattg atttctaagt ggataaaaga aggtaaagaa gtgttgagaa tttctgttaa
    37801 tgtatcacgt caacatctga atgataaatt gtttttagaa aaatataagc atatatgcaa
    37861 taaatacaat gtaccaacat gtctgataga tttagaactt accgaatcta ttttcttaga
    37921 aaatccagaa gctatagata taatagagga tattcattct aatggattta aatgttctat
    37981 agacgatttt ggatttgggt actcatcatt aggaatatta aaggatttta aagttgacat
    38041 aattaagtta gaccgttcat tctttgttag caaaaataat atagaccgtg gtaaggtagt
    38101 tattaagtct attattgaac ttagcaaaag gctaggtatg aaagtaactg ctgaaggaat
    38161 tgaagaatta gaacaagttg agtttctaaa aaatgtggga tgtgattata ttcaaggtta
    38221 tgttttttca aaaccattat taatttctga ttttgaaaat tttgcatatg aaaataataa
    38281 aattaaaaat atccattatt aaatgtacaa gataaaaaat ttaggcaagt tatataatta
    38341 aaa
//
LOCUS       DAYASV010000022        37851 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYASV010000022 DAYASV010000000
VERSION     DAYASV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37851
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.022"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            122..505
                     /locus_tag="ACGI68_001166"
     CDS             122..505
                     /locus_tag="ACGI68_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="HFH0137613.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYNG"
     gene            1013..1396
                     /locus_tag="ACGI68_001167"
     CDS             1013..1396
                     /locus_tag="ACGI68_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFH0137614.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            1398..1748
                     /locus_tag="ACGI68_001168"
     CDS             1398..1748
                     /locus_tag="ACGI68_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137615.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(1849..2307)
                     /locus_tag="ACGI68_001169"
     CDS             complement(1849..2307)
                     /locus_tag="ACGI68_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137616.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLEEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(2352..3140)
                     /locus_tag="ACGI68_001170"
     CDS             complement(2352..3140)
                     /locus_tag="ACGI68_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0137617.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQEGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(3279..3716)
                     /locus_tag="ACGI68_001171"
     CDS             complement(3279..3716)
                     /locus_tag="ACGI68_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HFH0137618.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            complement(3950..4084)
                     /locus_tag="ACGI68_001172"
     CDS             complement(3950..4084)
                     /locus_tag="ACGI68_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137619.1"
                     /translation="MGILEGAAMIREIAIKIAKEKGITEQKAWPEAVKEFKEKYELVL
                     "
     gene            4538..5047
                     /locus_tag="ACGI68_001173"
     CDS             4538..5047
                     /locus_tag="ACGI68_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137620.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGGLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            5074..5220
                     /locus_tag="ACGI68_001174"
                     /pseudo
     CDS             5074..5220
                     /locus_tag="ACGI68_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5453..5989
                     /locus_tag="ACGI68_001175"
     CDS             5453..5989
                     /locus_tag="ACGI68_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFH0137621.1"
                     /translation="MQGGVNIRQVKSFVFPVISLIFLDQISKVLIGLFLMDFEIDIIG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVVIGILHN
                     REISMKEYIYFCYRQFKK"
     gene            complement(6416..7216)
                     /locus_tag="ACGI68_001176"
     CDS             complement(6416..7216)
                     /locus_tag="ACGI68_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137622.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(7411..9009)
                     /locus_tag="ACGI68_001177"
     CDS             complement(7411..9009)
                     /locus_tag="ACGI68_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0137623.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINTLESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            9337..9513
                     /locus_tag="ACGI68_001178"
     CDS             9337..9513
                     /locus_tag="ACGI68_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137624.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            9687..10817
                     /locus_tag="ACGI68_001179"
     CDS             9687..10817
                     /locus_tag="ACGI68_001179"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFH0137625.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            11058..11408
                     /gene="eutS"
                     /locus_tag="ACGI68_001180"
     CDS             11058..11408
                     /gene="eutS"
                     /locus_tag="ACGI68_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFH0137626.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            11416..11865
                     /locus_tag="ACGI68_001181"
     CDS             11416..11865
                     /locus_tag="ACGI68_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFH0137627.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            11967..12542
                     /locus_tag="ACGI68_001182"
     CDS             11967..12542
                     /locus_tag="ACGI68_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0137628.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            12535..13944
                     /locus_tag="ACGI68_001183"
     CDS             12535..13944
                     /locus_tag="ACGI68_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HFH0137629.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            14128..15561
                     /gene="eutA"
                     /locus_tag="ACGI68_001184"
     CDS             14128..15561
                     /gene="eutA"
                     /locus_tag="ACGI68_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFH0137630.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            15579..16943
                     /locus_tag="ACGI68_001185"
     CDS             15579..16943
                     /locus_tag="ACGI68_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HFH0137631.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            16956..17837
                     /gene="eutC"
                     /locus_tag="ACGI68_001186"
     CDS             16956..17837
                     /gene="eutC"
                     /locus_tag="ACGI68_001186"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HFH0137632.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            17857..18510
                     /gene="eutL"
                     /locus_tag="ACGI68_001187"
     CDS             17857..18510
                     /gene="eutL"
                     /locus_tag="ACGI68_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFH0137633.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            18521..19222
                     /locus_tag="ACGI68_001188"
     CDS             18521..19222
                     /locus_tag="ACGI68_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFH0137634.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            19225..20694
                     /locus_tag="ACGI68_001189"
     CDS             19225..20694
                     /locus_tag="ACGI68_001189"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HFH0137635.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            20790..21077
                     /gene="eutM"
                     /locus_tag="ACGI68_001190"
     CDS             20790..21077
                     /gene="eutM"
                     /locus_tag="ACGI68_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFH0137636.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            21204..21965
                     /locus_tag="ACGI68_001191"
     CDS             21204..21965
                     /locus_tag="ACGI68_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFH0137637.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYRYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            21978..22607
                     /gene="eutD"
                     /locus_tag="ACGI68_001192"
     CDS             21978..22607
                     /gene="eutD"
                     /locus_tag="ACGI68_001192"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization phosphate
                     acetyltransferase EutD"
                     /protein_id="HFH0137638.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            22649..23374
                     /locus_tag="ACGI68_001193"
     CDS             22649..23374
                     /locus_tag="ACGI68_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HFH0137639.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDEFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMKGKEVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDVLNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            23387..23659
                     /locus_tag="ACGI68_001194"
     CDS             23387..23659
                     /locus_tag="ACGI68_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HFH0137640.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            23652..24203
                     /locus_tag="ACGI68_001195"
     CDS             23652..24203
                     /locus_tag="ACGI68_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFH0137641.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKVCAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            24215..25306
                     /gene="eutH"
                     /locus_tag="ACGI68_001196"
     CDS             24215..25306
                     /gene="eutH"
                     /locus_tag="ACGI68_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFH0137642.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            25308..25781
                     /locus_tag="ACGI68_001197"
     CDS             25308..25781
                     /locus_tag="ACGI68_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0137643.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(25901..25996)
                     /locus_tag="ACGI68_001198"
     CDS             complement(25901..25996)
                     /locus_tag="ACGI68_001198"
                     /inference="COORDINATES: protein motif:HMM:NF012392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0137644.1"
                     /translation="MGYPNVEHFIRLFNKKYNMSPSKYRKNTSKI"
     gene            26297..26818
                     /locus_tag="ACGI68_001199"
     CDS             26297..26818
                     /locus_tag="ACGI68_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0137645.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            27197..28372
                     /locus_tag="ACGI68_001200"
     CDS             27197..28372
                     /locus_tag="ACGI68_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137646.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDTLKLGCEFMAKYAVEFLNKNNN"
     gene            28407..29945
                     /locus_tag="ACGI68_001201"
     CDS             28407..29945
                     /locus_tag="ACGI68_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0137647.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            29977..31179
                     /locus_tag="ACGI68_001202"
     CDS             29977..31179
                     /locus_tag="ACGI68_001202"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0137648.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQKKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(31346..31945)
                     /locus_tag="ACGI68_001203"
     CDS             complement(31346..31945)
                     /locus_tag="ACGI68_001203"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137649.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            32115..33473
                     /locus_tag="ACGI68_001204"
     CDS             32115..33473
                     /locus_tag="ACGI68_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137650.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            33466..34098
                     /locus_tag="ACGI68_001205"
     CDS             33466..34098
                     /locus_tag="ACGI68_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFH0137651.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            34427..35584
                     /locus_tag="ACGI68_001206"
     CDS             34427..35584
                     /locus_tag="ACGI68_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFH0137652.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFTLIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(35731..36366)
                     /gene="lexA"
                     /locus_tag="ACGI68_001207"
     CDS             complement(35731..36366)
                     /gene="lexA"
                     /locus_tag="ACGI68_001207"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFH0137653.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(36470..37597)
                     /locus_tag="ACGI68_001208"
     CDS             complement(36470..37597)
                     /locus_tag="ACGI68_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0137654.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
BASE COUNT        14682 a         4684 c         6224 g        12261 t
ORIGIN      
        1 aaaaaactat attatactat cttttctttt tggaataaac tatactttaa atatagatat
       61 ttaatttaaa taaattttac agtattaata gtaaattatc ttagaaagga atgtgcttta
      121 tatgtctaca gacaaagttg ttggaatcat agatgaagaa acggcagaac ttgcaggaat
      181 tgaatatact ggtaaaattt atgcttcttc tggtgttata aaacatataa agaaaaaaca
      241 tagatgtcaa ctgagtaagg atatttttaa tgatataata gacactataa aaatggtatt
      301 aaagtcacct gaatatatag gaagtcatcc taaaaaacct ggaaaaagtg tggagtttat
      361 aaaaaaaatt gatgattata ttttagtagc tacagaatta gatacaaaaa aaggatattt
      421 atatgtatct tcaatgtatg ctataaaaga agcaaaatta gaaagtagag taaacagtgg
      481 aagagtaaaa caatataatg gttaacataa tattattaat tttccctaaa atattgaaaa
      541 atattattat ataagttata atattaatat acacgtagat atttattagt tagatttgag
      601 gttggaaaag gcacccaata cgctactata gagtagttaa tagagatgca ggatacgccg
      661 ccctgccaaa tctaattaaa ttaaaaagag aggattaatt cctctcttat tgtttttttg
      721 agatactaat ttaatctata atagaaagat tgaaggtcgt ttatacaaaa aaattttcag
      781 ttatatacat taaactaatt aaataagata tttataatct atatgtgata catattatat
      841 attaataaaa gaaaaatata tagaaaatcc ttataaaaat ttaaattgta atgaatagga
      901 agtaattaaa aagaattttt attgattttt aaaaatttag ttagaaacta tatcattatg
      961 gtaaaaaatg tattataatg tttagaaata ttataataag gagcgattaa acatgaatct
     1021 atcgaagaaa atagaagata aaagaaaagc taaacagaga gctgaaaaag ttaagaaagc
     1081 taaaattgct acagctggtg tggtattagg agctgttact ggtgcagtga gtggagtact
     1141 tttggctcct aaatctggaa aagaaacaag agaggatata aaagatgctt cacaacaaat
     1201 tgctgaaaag ataaatatga aaactgtaga tgtaaaaggc aaagtatctg agaagctaga
     1261 ggataaaaaa ggaaacttta tagaatcaaa gaaaaaaata aaaaaatata tagataacaa
     1321 aaagagtgtt gaaaattcta gtgaggaaga taaaataata gatatagttg aaccagttag
     1381 tgttactgag gaataatatg gacaatacaa taacgattaa cataagtata atatatgtat
     1441 tattagctat acttgcttgt attgctttag tttatttaat tattgtactt gttaaactac
     1501 ataagatttt aaataatgtt ggaactatgt taaatgaaaa tactcaaaat ataaatacaa
     1561 ctttaaataa tcttcctaat gttgtagaaa cttttggaga agtaggagaa aatatgaaag
     1621 atgtaacaga agtagttact gaagttacag cagattttct tgttactaaa gacagtgtca
     1681 aatcaaatat ggatgttata gttgatatat tgactataat taaaaacatt tttctagata
     1741 aaaaataata agtttaatta aaataagcct ataagatata atttaaacga ccttaaaata
     1801 gcatttataa ggtcgttttt tattagcttg atttgacact atgtgttttt aataattggt
     1861 aatattcctc tttatcattt gaacatatat atctatgctt gttgtttcaa ttacatccat
     1921 aagaacatct ttacattcaa gctcaaaatc ttccattcct tctaaatatt catcatcttc
     1981 atcaaaaaag atataagaat tatatagcca ttcttcaaat ttttcttcaa gtatatccca
     2041 atacgaactc caaaataatc cacaacctga tatataagat gctctacttt gatttatata
     2101 aacctcctct agaagctttt gtaatctact gctataaaga aaatctgctc ttccactgct
     2161 tgatattaat tcatttaaat cttctattga cattaattca actttaccac ttttatcata
     2221 aggagtataa ttttcatcaa cccatttatg tggtttgttt tctctgcata ctgttgctat
     2281 caactctaca acttttgttc ttatcatttt tttatcctaa cttatttatt ttttataaaa
     2341 taatcatgat tttataattt atctatttct tgtaatgcta atttaacagt ttcatatcta
     2401 tcagcaccat ttattactac atcacctttt acaagtttat tagcttctcc acctacaaca
     2461 taaacctttt tacctaaacc ttgcttataa ctagacgctt caattattaa agaatctttc
     2521 aattgccaat gtaaaatctg agcagccact ttatcagcat cacctgtata aacgattaca
     2581 ttttcataca ctttattttc ccctccctct tgagaatttg atatagattt gtttagtata
     2641 ccttctgcta tcaatttagc aactttatct ttattattaa cataataatc tgtatctgct
     2701 ttactatcta caaaacatac ttctattaat attgctggtg caatagtatg attaagccaa
     2761 taaagacctc taacatcaga ctttgcgccc ctatctttga atactgtagc taacctagta
     2821 ttcactcttt ctgcataggt tttgccatta ttagtcttat atattgtttc tgtccctact
     2881 ggagttgaag ttgtggcatt tgaattaaaa tgaatctgaa cagccaaatc agtttcctgt
     2941 ttatttgcta tagcacattg gtctgctaaa tgattacttg attcatcaac tcttcctgta
     3001 taaacagtag ctccacctat tttaagccat ttaacaatta agtcattcag tattcttgtt
     3061 tctttgcttt cgtttatata cccaacagct cctgtacctt ttccaattaa agtgtgccct
     3121 ggtattatta ctactttcaa actaacatct cctttacaat taaatttaac ttctatttaa
     3181 tatatgcata acctagaatt ttgtttcttt ttatatctta tacttttatt ataacccatt
     3241 ttgtaaaaat atagaatatt aagtaaataa tgttaaaatt ataattttgt atataagaca
     3301 ttacttgatt gcaataattt tatagcatat tcatctattc tatatccagt tctataatat
     3361 atatgtttaa ttccagcttg aattatagcc ttagtacaat ttaagcaagg aaagtgagtt
     3421 acataaatag agcaattttt aactgaaata ccttcttttg cacaataata aagggcattt
     3481 tgttcagcgt gaatggttct aatacaatgt ccatctctca tttcatgacc aacttcacta
     3541 caatgcttat ctccagaaat actaccatta tatccagtag acactattct gttttctgaa
     3601 ttgacaatta tagctcctac ataagctcta tcacaagttc ctctttcagc tactgtttcg
     3661 cacagtctca taaaatattc ttgccatgag catctatttt caagtttttt attcaaatta
     3721 tatacctcca aaacttatat ctaatatata agatttatac cttaaaaata atattaaaaa
     3781 caaatttata cgataaataa aatttattaa tactgttttt agtctaaatt atttcatttt
     3841 aaattaaagt tacaaggaat ctaaaattat atatatcttt cttcgtaata ttaatgttac
     3901 aatataaaaa actgtctcaa taaaatagag acagttttac agtcaaagtt tataatacaa
     3961 gttcatattt ttctttaaat tcttttacag cttcgggcca tgccttttgt tctgtaatac
     4021 ctttttcttt agctatttta attgcaatct ctcttatcat tgcagcgcct tctaatattc
     4081 ccatttttaa tctcccttca aatatctttt tgtatagaat ataaatagtg ctaagattta
     4141 ttacatgctg catatacaat atctatacaa cacataactt gtaaattcct tttattttca
     4201 tacgtcttta accgacatat aattatattc aataagtttt aaaattatga actcaattat
     4261 aattattttt atttttttag aatctaaaca ctaaaaattg taggtaaatt tgtcttatgc
     4321 ctttataaat atttctacag ttttaattac tggacatatc tatttttatt ataaaaagcg
     4381 attaaaaatc gagaatatcc gcataaaaac tgtatataag caaaacggtt aaaggaaaaa
     4441 gaagaaattt acttgaatgt taaaatgcgt tgcttgaagc aatgggcagt tttgcctata
     4501 atatcggtaa caactgaaag aaaggtggtt agccctaatg ttaagcgaaa acattaaaac
     4561 aatcaggaag tcaaaaggac tttcacaaca agaacttgct ataaaactga atgtagtgcg
     4621 acaaacaata tctaaatggg aacaaggact gtcagttccc gattctgata tgttgatttc
     4681 catatcagag gtgcttgaaa cgcccgtaag tactttactt ggagaaacta ttgttgagtc
     4741 ggaagttgat aacttaaaag tgatttctga aaaattagag attataaact tgcaacttgc
     4801 acaaagaaaa aacatgagaa gaaaaatgtt tcattggtta tttattttat tatgtgcagt
     4861 catattaata gtttttgtgg tcttaggggg actaaatagt ccttacttgg attggaatta
     4921 taccgaccct gaaaccgctg tttttggagt agcttttcat gcttttgagt ggctatttgt
     4981 cagattagta ccgattgtcc ttataggagc aattattgga atctttttga cacgtaagaa
     5041 aatatgaagt aactctgctt tttataatac agcaagcgtt ccttaaaagc gtcattctac
     5101 atggcaatcg aggacgacta tgtaaggaag aacctttttg atttccaact aagcgaagtt
     5161 attgaggacg acagtaagac caaaaccgca ttaagtaaag tacaggaagc aagactgctg
     5221 gacttatact ctcatgcgtc agatgcagga aatgcgaggt ctggtagcat gatttacaac
     5281 gatttgtaca acgactgcta gtagaaataa tatggagata agtattatct acaaagtcag
     5341 aaaagaaatg acttgataat ttactcatgt tataattatt gatgtgaaat taataaatcg
     5401 aaattccttt tttggattta ataatactga gtaaggaaaa agtgaattac ttttgcaagg
     5461 aggtgttaat atcaggcaag taaagtcttt tgtttttcca gttatatcct taatattctt
     5521 agaccaaatt agcaaagttc ttataggatt attcttaatg gactttgaaa ttgatataat
     5581 tgggaaattt ttaagattca atcctgttca aaatacaaat ctctcttatg ggggaaactt
     5641 tattggtatt ctatctaatt tatgggtatt ggtattgttt aacattttag ttatattagt
     5701 tattatatct ggatatgctt tttataaatc aaaaaatgaa caaacaagct attcagtaaa
     5761 agtaattatg tcttgtggac ttgctggtac aatatgtagc ttgatagata aattattttg
     5821 gggaggaagt ttagattttt tgcagatacc aagctttttt attttcgact taaaagactg
     5881 ctaccttact gttgcagaaa taatatttgt tgtcatagga attttgcata atagagaaat
     5941 atcaatgaaa gaatacatat atttttgcta tcgtcagttt aaaaaataaa tttagaagat
     6001 aaatttaagt ttcaaggtaa acttatttaa tcacagcaaa tagttggctt acttaagctt
     6061 ttgggctaaa ctgtgagcac taattaagtc tctgatatct aacaaacgga gcacaaaaat
     6121 aaaaattaaa tataaagcac agttaatttt gattaaaaag ttgactgtct tttcttttgt
     6181 agaaatttaa gcataggtaa taaagaaatg actgcattta agcattatgt atataattgg
     6241 aggcaataaa aagccagcaa agcccataaa atagagctgt gctagcaaca tgatgaatta
     6301 taagaagata atcaaaaact tacgctttat tttaatttaa aaaaatgatt actgtaaaga
     6361 aatcattttt tattataatc actgatacat caaatattct aaataaaatt tttatttatc
     6421 tggaattgta tattttttct ataatattaa ctacagcgta cattgcccaa gcacagatag
     6481 ccaatacaaa tactcccatc attactaaat ctaatttaaa tacttgagaa ccatatacta
     6541 tcaagtagcc tattccatat cttgaaacta aaaattctcc aactattaca cctacccatg
     6601 ccataccaat attaatttta gttaaattta ttaaatttcc tgtgtttgca ggtaatatga
     6661 gtttaaataa tatttgagat ttagtagctc caaaactttt taacatctta attttttctt
     6721 catctatatt cataaaataa ttataagctg aaagtatagt aacaacaaca gaaatcgtta
     6781 cagctgttac gacgattcct tctattccag cacctaccca aactataatt ataggagcta
     6841 aagcagtctt aggaagagca tttaaaacta ccaaaaatgg gtctaatatt tttgataatt
     6901 tttctgacca ccaaagagca attgctacca aaatacctaa tacagtacct ataacaagac
     6961 caagaacagt ttcatataca gatattccaa tatgtttaaa taattgacca ctaaatgcat
     7021 attgaataaa taaattgtaa atatcacttg gtttgctaaa tagaaatgta tttataacat
     7081 ttaagtcagc taaaagttcc cagattacaa taaaaccaat taaaattaat agctggtaga
     7141 ataatacatt tcttttttct ctttttacac ctttaacata gtttatatat ccttcagatc
     7201 ttttattaag cctcatgtgt atctagctcc ttccataata tgtcgaaata cttttgaaaa
     7261 tcaggaactt tacgtgtttt gagaggcgtt tttgttccat ccatgttaag ttcaatatcg
     7321 taaatatttt ttatggtagc tggtctttta gatagtattg caaccttatc tgatatgctt
     7381 attgcttctg ctatagctaa aaaagtgcaa ctaattgctt aaagaaacag aattatcaac
     7441 ttcagcaccc atataaacaa tattaagatt tcctaaactg ctatcccaaa cgatactatt
     7501 aataatagta gataataaca atctcttatc atctaaactg gcattatcaa cttcatcatt
     7561 aaatctcttc aaattactaa caataatatc aatattaata ctgtcactac taatattgat
     7621 gttactagat tccaaagtat taatcttatc ctttaaaata ctaatctcat tatttaggct
     7681 ctcaatctgg gaaactatat aatcagaagc agtactacca gtattttttg caagctctat
     7741 aactaaattt ttaataagtc tttccttatc taaaatttga tttttaagta aattagtctc
     7801 agatttaaaa atattaaaaa cattatcagt cttaatgtta ttactattga atgcacttat
     7861 aatagactct tctttacaat tcttaatttt atctataact aaactttcaa ctaatggacc
     7921 attaagattt ttacagttgc aactatcaga cccaagtgtt tttttagtac cacaaatata
     7981 ataatacttt aaatccttgt cagttttttt gcctttatag gtaattctca tattagaacc
     8041 acatttacca cattttataa gaccagataa aagagcagtt tttccagttg atatccttgg
     8101 agttttatca cgatttttat ctaattgatt ttgtacttca agccatattt caggttctat
     8161 gactccatgg tgttttgcaa ttgctgctat tgagttagat gtatttttag cataggtaag
     8221 gattccacat tttctatctg gagtacctac aacatccata ccactatttt taaggtaatc
     8281 taaaatttca tcattagctt ttacatatgc tggatttcta agtattctag ataaagaact
     8341 agggtcaaat ttgccaccac gaggacccct tattccatta ttatagacat atttatataa
     8401 cttagataag gacctaagtt ctaaatattt attgtatatt agctttacaa tctccatttg
     8461 ttcatcattt acttgaagct tatacatact tctttctttt agattttcat ctaagaatgt
     8521 gattttttca ctgttgaatc catgaggtgt attgcctcca agccatctac cactacgggc
     8581 taattcatac atattgtctt ttatacgttc tgctattgtt tctctttcta attgagcaaa
     8641 tacacttgat ataaacatca tagctcttcc cattggagtt gaagtatcaa attgttcttt
     8701 gatagatata aatgatatgc ttagttcatt taatttttct attaaagtag agaagtcaga
     8761 tacatttctc gatatcctat ctaatctata acaaactagg taactaaatt tcttatcttt
     8821 ggcatcctgc atcattttct taaattgtgg tctatctata ttgctaccac taaatccctc
     8881 atcttcatat atcaaaaaat cttttacacc aatagacttt gcataatcaa tacataattg
     8941 tatttgattt tctatagaat caccttttcc agtaaatctt gattttcttg aataaattgc
     9001 agctttcaaa attaaaaccc cctataagtt aggatatata tttgaaacta tttatctaca
     9061 accttttaag taaacttatt agaatgaatt tattaattag gtaaatttat ataattctac
     9121 tttttataat atatgactat ttattttatt tctttactat tcaagttcta aatcaataat
     9181 aaattttgat atttaattgt atcaggacaa atctctaatt tcatagatta ttaatataaa
     9241 atcataggtt agtgtgaaaa agttgaaatt taaatatttt aaaagataat ttttttgaac
     9301 taataggtat aacaaaaatt tataggggga ttgcatatgg aaatcaatgt tatagaaatt
     9361 ttccctaaag ataaagctaa acttaataaa atagaaatgg ataaagctag ttggtttgta
     9421 aatataataa gtaaaaaata tcctaaagaa gctttaaatg aagcatttag tactttagaa
     9481 aaagaattaa atataagtaa agctaataca taagctttga ttattgattt tatatactat
     9541 tggttttata cgctatgact tgacattata ttaatcaaat agtatatttt taaatatagc
     9601 acaatgaagt gttaaaagag caatggagct ccatagataa agtctatgga gcttttttgc
     9661 gtatttaact taaggaggat aggactttga ataactttga gattaaaact agaataaaat
     9721 ttggagaagg ctcactaaaa gctttgcaag aattaaaaaa caaaaatgta ctaataataa
     9781 cagacccttt tatggtgaaa tcaaaaacaa tagataaaat aacatctaat ttaataaact
     9841 caacatatca agtttttgaa gaaatagtac cagacccacc aatagaagta gtagtagcag
     9901 gaatagaagc atttaaatct ataaatccag acgtaataat agcattaggt ggtggttctg
     9961 caatagatgc agcaaaatct ataatggact tttctaaaaa aatattaaaa attaacgaag
    10021 tagagtttat agcaatacca acaacaagtg gaacaggttc agaagtaaca tcatttgctg
    10081 taataacaga tagtcaaaaa aaatgtaagt atccattagt gagtgatgat ttacttccag
    10141 atatggcaat actagaccca gaacttgtaa aaactgtacc aaattttata acagcagata
    10201 cagggatgga tgtattgaca catgctatag aagcttacgt atctacaaat tctaatgact
    10261 tttcagatgc actagctgaa aaagctataa agttagtatt taaatactta ataaaagcat
    10321 ataaagatgg aaatgacata gaagctagag aaaaaatgca caatgcatct tgtttagcag
    10381 gtatggcatt taaccaagca tcacttggca taaatcattc tattgcacat gtattaggtg
    10441 gaaaattcca tataccacat ggaagaacaa actctatatt acttcctcat gtaatagaat
    10501 ataattcatc aatagtagga tatactaaca caacttactc tgctacagct aaaaaatatg
    10561 cacaaatagc agaattagta ggacttggga gtgctaatat aagaatatct gttagaaatt
    10621 tagtaaatga aataaagaaa ctacaaaaag aaatgaatat gccaactaaa ttgacacaat
    10681 gcaaattaag tttagaagat ataactaatg aagaaagaga aatagcaaaa cttgctataa
    10741 aagatacttg tacattaact aatcctaagg taccaactga agatgatata ataaacatat
    10801 taaagaacat taaataacta cattttatta catgtaaata taatattata caaataggga
    10861 aaatgttttt aaaaatataa attatatatt gacaaatata aaacataaaa ctataatgaa
    10921 agtatcacaa aggcgtgata ctttagtaga aagcaatgga gcttcttagg aatattaata
    10981 ttattcttat ggagctcctt ttattttaat caaatagctt tttaataaac tatattcaaa
    11041 gaaagcaggg aataaatttg actgaagaat ctaagcaaag agttattcaa gaatatgttc
    11101 ctggaaaaca ggttacatta gcacatatca tagctaaccc taatgaagat atatataaga
    11161 agttaggatt agtacttgat aaaaaagatg caataggaat acttacaata acaccaagtg
    11221 aagcgtcaat aatagcagca gatgttgcta caaaggcttc aaatgtatct ttaggtttta
    11281 tagatagatt tagtggttca gtagtaatat caggtgatgt aagttctgta gaatcagcgt
    11341 taaatgatgt tttagaagtc ttaggaaata tgttaaactt ctcatctaca aaaatcacaa
    11401 ggacataata atacaatgaa gaatataata tttatgggaa agacaggtag tggtaaaact
    11461 accttatgtc aaaaactaga ccagttagaa ttaaagtata aaaaaactca atcagtggaa
    11521 ctttataata atgcaataga cactcctggt gagtatatgg aaaatagaaa cctatataat
    11581 gccttaataa caacatcagc agatgctaaa attatagcaa tagtgtatga tgccacacaa
    11641 gaagaaaatt atatagcacc aggttttgcc agtatgtttt gcaaagacgt aataggaatt
    11701 ataactaaaa taaataaagc tactgaaaac gaagtcaata tagcttatga aagactatca
    11761 ctagcaggag caagtcaaat ctttaaaata gacacagttg ataatattgg acttgaaaaa
    11821 ttatttaaat atctagaaaa atataaaaat atagatgaag tttaagggga aatatttttt
    11881 tattcaaaaa tattattaaa gtatgataaa ataaaaaaat gtataaacta ttttaagaag
    11941 atttaatctt taagcgaggt aggaacatgg ggaaaaaaat actattggtt gatgatgaac
    12001 cactgttaag gatggataca aaagatatat tagaaagtca tggctatgaa ataataggcg
    12061 aagcaagtga tggatttgaa gctgttgaaa tgtgtaaaaa acacaaacct gatttagtta
    12121 taatggatat agagatgcca atacttgatg gtataaaggc aggcaaaata atatgtaaag
    12181 aaaatatagc tggtggagta cttttattaa cttcatttga agataaagaa tacgtagaaa
    12241 aggctaaaag tataggtgct tatggatatt tagtaaaaac agcaagtgaa aaatcactta
    12301 tcccaaccat agaaatgtgt ttaaataagg taaatgaatt tgaaaaaatg aaaaaagatt
    12361 tagaaaaagt aaattctaaa ttgaatgaaa gaaaattagt agaaagagca aaaggtatac
    12421 ttataagaga gtttaagatt tctgaggatg aagcatacac tagaataaga aaattaagta
    12481 tggataaaag aactacaatg atggaaattg ccaaaatgat tataatagga tacgatgact
    12541 aatacgataa aaaaaatatc aactgcacaa acatacttaa ataataacga aattgaaaaa
    12601 attatagaag tatcaaaatc cttagattta atggcttctt tttatgaagc tgatgttttt
    12661 atagatgtac taagtaaatt tgatgatgaa gcaattgttg tggcacatgg tagacctaaa
    12721 gaaaagtcaa tgtactgtga aaatgttata ggtaaaaagg ctttaattga aaatgaacca
    12781 ggagttataa agtgcttaaa aacaggagaa agagtaagag atattaaagc tttaacacag
    12841 gaatataaac tggtcaaaca aaatattcag ccaataacac tagatgacaa agttttagca
    12901 gtactaatag ttgaaaaaga tataagtagt gaagttaaga atgagtttaa tgctagtgaa
    12961 gacgaagaaa ataaaaattt acatcagttt ataaaactta ttggaaataa tgacttttta
    13021 gttgataatt taaattcagc catattaata tttgataaaa atggaaaatt aaaaattaaa
    13081 aatcgtattg caactgagat atacaaaaat cttgggtttg gatatgatat acaagatatg
    13141 cattacaatg atttagatat agaaaccaac acctttgaaa atatattaat ggatgaaaat
    13201 tatgatgaga caaatgaagt ttcaataggt aatatgtttt ttaaaataaa gaccatatac
    13261 atcaaagatg atgaatttaa ggtttgcaaa atagtacaag atataagtga tttaaagaga
    13321 aaagaagaag aattagtttt gaaaacagtg gctataagag aagctcatca tagagtaaaa
    13381 aataatcttc atacagtaat atctataata aaaaaacaaa gtagattatc tcaaaatgat
    13441 gaagtaaagc agtgtttaga taacataata aatagggtat ttgcaatact ttcgactcat
    13501 tatttactat ctaaagaagt tgataataat atatctatac gagaaggtat caatttatta
    13561 gtgtcaaata ttcaaggtgg ctatttattt agtaaggata tagatatacg tataactggt
    13621 gaagatttta aaattagtgg agaaaaagca acagcaatac ttttggttat aaatgaaata
    13681 ctacaaaatt gttatgacca tgcattttcg gatagagaac atggtactat acaaatatta
    13741 ataaataaag ataacgacta tagaaatatt gctattatag atgatggaat tggatttgat
    13801 agtaataata taaataaaaa tagcttaggg acatatataa tagatagtta tataacacag
    13861 atgttaaaag gaaatataga gagaaaaagt agtgagaatg gtacagaagt attaattact
    13921 atacctacac aaattaatat ttaaataata actcatctac aaagtagtag ttttaattga
    13981 gcaaagaggc ttaaggttat taatagatta agttaattga atgtatttat aaaaattata
    14041 agtatatatt caatttaaca ggtatttatt ataactttaa gcctctttgc ttgtttaaga
    14101 catacttaaa ttttaggagg tgagaaaatg agagaagagt tattaagtgt tggtattgac
    14161 attggaacaa gtactactca gttaattttt tctaaattaa tagtagaaaa tactgaatca
    14221 aatttttcgg tacctagaat aagtatagta gacaaagaaa ttatttataa aagcaaagtt
    14281 tatttcaccc ctcttatttc aaataatgaa ataaatggtc aacaaataaa agatatagtt
    14341 gaaaatgaat ataaaaaagc aaatatatct aaagaagata tacatacagg agcagtaata
    14401 ataactggtg aaacagctag aaaagaaaat gcaaatgatg tacttcatac tttgagtgga
    14461 tttgctgggg acttcgttgt tgcaacagca ggacctgatt tagaaagtat aatatcagga
    14521 aaaggagctg gagctcatat ttattcaaaa gaacattcaa ctacaattgt aaatatagac
    14581 ataggtgggg gaactagtaa catagcctca tttaaaaggg gagaagtaac cgatactggg
    14641 tgtttggata taggtggaag gcttgtaaag atagataaaa taagtaaaaa aataacttat
    14701 atatcaccta aaatagataa aatcataaaa gataataatt taaatcttca agtaggcaca
    14761 atggctactg tagaaaatac aaagccaata gtggatataa tggttgactt attaatggaa
    14821 agcataggat taaagaaaca gacacctttg tttgatttta taataaccaa taaatcaata
    14881 agtatagatg aaaatattaa aaatgtttca ttctctggtg gagtagctga ttatatttac
    14941 tatgatggag aaataaaaga ttattttgaa tatggtgact tgggaatttt acttgggcaa
    15001 gcaataaaaa attgtgaata ccttaaaaaa gtaaatgtag ttaaatctat ggaaacaata
    15061 agagccacag tggttggagc aggttctcat actactgaag taagtggaag tacaataact
    15121 tacactagcg aaaagtttcc acttaagaac ttgccagttt taaaattatc aattgatgaa
    15181 gaaacaggca cttcaaaaca aatcagtgaa gctataaaaa ataaacttaa ttggtttaaa
    15241 ctagaaaatg atttacagaa agttgctata gccatgatag gaaagaaaaa tccaaccttt
    15301 ttagatatac aagagtatgc taaagggttt gtagatggaa tgcaacaaat catagaaaat
    15361 gatttacctt ttatagtaat tgttgaaaat gatatggcaa aagtgttagg tcaagcaata
    15421 tatgcattat taaattatga aaaagaaatt gtttgtatag acagtgtcaa ggttgaaaat
    15481 ggagactata tcgacattgg aaaacctata gttgaaggta aagttttacc tgtggtaata
    15541 aaaacattag tttttaatta atagaaggag gtaagtaaat gattttaaat acaaaattat
    15601 ttggacgtac ttatcagttt aaaggcttat atgatgtaat ggctaaagct aatgaagaaa
    15661 aatcaggaga taaattagca ggtcttgcag cagaatcagc tgaagaaaga atagcagcaa
    15721 aagttgtttt atcacacata aaattggagg atataagaaa taatcctgca gtggcatatg
    15781 aagaagatga agtaacaaga ataatacaag atgctgtaaa tgaaaaagtt tatgaagaaa
    15841 taaaaaattg gacagtctct gaacttagag aatggctatt ggattcaaat actacaagtc
    15901 ttgatataag aagaatatca agaggattaa cttctgaaat gatagcagca gttgcaaaat
    15961 taatgtcaaa tatggactta gtttatgcag ctaaaaagat aaaggttact gctcactgta
    16021 atacaactat aggagaacaa ggaacattat cagcaagact tcaacctaat catccaacag
    16081 acgacccaga tggaatactt gcatcacttc ttgaaggttt aacttttggt ataggagatg
    16141 cagtacttgg attaaatcca gttgatgact ctgttgagag tgttactaaa gtattaaaaa
    16201 gatttgaaga agtaaaacaa aaatatcaaa taccaactca aacatgtgta ttggcacacg
    16261 ttactacaca aatggaagca ataagaaatg gtgctcctgc agatttaata ttccaatcta
    16321 tagcaggttc tgaaaaagga aatgaagcat ttggatttaa tggcgacact atagaagaag
    16381 ctagacagtt agccttaaaa cacggtacag ctacaggacc taacgttatg tattttgaaa
    16441 ctggacaagg ttcagaatta tcttcagaag cacatcatgg aatagaccaa gttactatgg
    16501 aagctagatg ctatggattt gctaaaaagt atgaaccatt cttagtaaat acagttgttg
    16561 ggtttatagg acctgagtac ttatatgatg ctagagaagt tgtaagagca ggacttgaag
    16621 accactttat gggtaaacta catggcttac caatgggtgt tgatgtttgt tacactaacc
    16681 atatgaaggc tgaccaaaat gatatggaaa acttagccat actattaaca acagcaggat
    16741 gtacgtattt tatgggaata cctcatggag atgatgttat gcttaactat caaacaacag
    16801 gataccatga aacagcatca cttagagaaa tgtttggact aacagcaata aaagaatttg
    16861 atgcttggat ggaaaagatg ggattctcta aagatggtaa gttaacagag ttggcgggag
    16921 acgcatctgt attattagga taggaggtat ttaatatgaa tgagaaggat ttaaaagcat
    16981 tggtagaaca attagttgga caaatggttg gagaattaga tactaatgtt gtgtctgaga
    17041 ctgttaaaaa ggcaactgaa gttgtagtag acaacaatgc ttgtatagat gatataacag
    17101 aagttgatat aagaaagcaa ttactagtaa aaaatcctaa ggatgcagaa gcttatttag
    17161 atatgaaagc aaaaacacct gcaagattag gtataggaag agctggtact agatataaaa
    17221 cagaaacagt tttaagattt agagcagacc atgctgctgc acaagatgct gtattctctt
    17281 atgtagatga agaatttata aaagaaaata atatgtttgc agtggaaact ttatgtaaag
    17341 ataaagatga atacttaaca agaccagatt tgggaagaaa attttctcca gaaactataa
    17401 ataatataaa atctaaattt ggtacaaatc aaaaggtttt aatattagtt ggagatgggc
    17461 ttagctcagc tgcaatagag gcaaatttaa aagactgtgt gccagctata aaacaggggc
    17521 taaaaatgta tggtatagat tcaagtgaaa ttttatttgt taaacattgt agagttggtg
    17581 ctatggacca cttaggtgaa gaattaggtt gtgaagttat atgtatgttg gttggagaaa
    17641 gaccaggatt agttactgct gaatcaatgt cagcatatat agcttataag ccatatatag
    17701 gaatggcaga agcaaaaaga acagttatat caaatattca caaaggtgga acaactgcag
    17761 ttgaagcagg tgctcatata gctgaactta taaaaactat gttagataaa aaagcttcag
    17821 gaatagacct taaataaagt cgagggagtg tagaatatga aaaatgatgt aattcgtcct
    17881 aatatactag gaattaaaat aatttcaaat ataagtccag aaatggcaca aaaactagaa
    17941 ttaaaatcac atcacaaaag cttaggttta ataactgcag actgtgatga tgttacgtat
    18001 acagctcttg acgaagcaac taaagcttca gaagttgatg ttgtatatgc aaaatcaatg
    18061 tatgcaggtg ctgcaaatgc ttcaacaaaa cttgctggtg aagtattagg aataatagca
    18121 ggtccaagcc cagcagaagt aagaagtggt ctaaatgcag tagttgattt tctagagtat
    18181 ggagcaactt ttataagtgc aaatgatgat gattcaatag catactatgc tcactgcgta
    18241 tcaagaacag gtacatacct ttcagaggta gcaggaataa gagaaggtga agcattagct
    18301 tatttagtag caccaccact tgaagcaatg tatgcactag atgcagcaat gaaagctgca
    18361 gatgttaaga tgtgtgaatt atttgcacct ccaactgaaa caaactttgg tggagcatta
    18421 cttacaggtt ctcaatcggc ttgtaaagca gcatgtgatg catttgcaga agcagtaaaa
    18481 tcagttgcag acaatccaac aggattttaa ggatagttag atggatgcta taggcttaat
    18541 agaagtaata ggatatgttg ctgcaataga agcttctgat gcttgtttaa aatctgcaaa
    18601 tgttaatatt gtaagagttg ataaggttgg agcaggtatt gttactttaa caatatgtgg
    18661 agatgtcggt gctgtaaaag ctgctttaga agcaggtgaa atagcagctt ccagagttgg
    18721 tacattaaga acatcacata ttataccgag aatccataat gaagtaacaa atgctttatt
    18781 caaaactaaa gaacctaaag tctgtgaagt ttcagaagat aaagaaaaaa actttgagat
    18841 tacattggaa atagctgata atgtatcgaa aaatttatca gaaaaagaaa atgtaatttt
    18901 agatgtcaat gaaaacagtg gagatactga aaatttatat agtgatatag ataaaacaaa
    18961 caaaaaaatt gaaacagttg atgatagttt aacagaagct aatgaggaaa aaatagaaaa
    19021 cactaatgat aaagaagtat tggaacaagt taaaatagaa aattccgatt ttgatagaga
    19081 tgacctatca aagaacagtg taaaaaaact taaagctatg gctaagaaat tagattcttc
    19141 aataacttat aaagacttaa atgttcttaa aaaagaagaa ttaataaact tagttaaaaa
    19201 gcttagtaga agggatagat agttatgaca cttttagata aagatttagt atctatacaa
    19261 gaagtaagaa ctcttgtaag aagatctaaa gtggctcaag agaaactttc taaaatgagt
    19321 caagagcaaa tagataaaat agtaaaagct gttgtagatg cagcatttaa taatgctact
    19381 aaattagcta aaatggcaaa tgaagaaact ggatttggaa aatgggaaga taaagtaatt
    19441 aaaaatactt ttgcatctat gggagtttat gaaactttaa agaacacaaa aactattgga
    19501 ataattggtg aagataaaga agccaatacg atagatatag ctacaccagt tggagttgtt
    19561 gcaggtctta taccttcaac aaacccaaca tcaacagtaa tatacaaaac tataatatct
    19621 ctaaaagcag gaaatactat agttttctct ccacatccaa atgcaaaaaa ctgcatactt
    19681 gaaacagtta atataataag tgatgctgct ataaaggctg gagctccaga ggattcaata
    19741 ttatgtataa ctaatccaag tatagttgga accaatgaat tgatgaaaca taaagataca
    19801 aacttaatac tagcaactgg aggagaagca atggttagag ctgcttactc ttcaggaaca
    19861 ccagctattg gagttggacc aggaaatgga ccagcattta tagataaatc agcaaatgtt
    19921 aaattagcaa tcaaaagaat aatagattct aaaacttttg ataatggtgt tatatgtgca
    19981 tcagaacaat ctatagtaat agaaaaatct atggaagaaa ctgttttaaa agagcttaaa
    20041 aatcaaggtg catatgtttt aaatgaggag gaaagtgaaa gattagcaaa attcatatta
    20101 agagctaatg gtactatgaa tccacaaata gttggtaaaa gtgttgaggt aatagcaaat
    20161 ttagcagact taacaaatgt acctaaaaat acaaaagttc tagttgcaag agaaaataaa
    20221 gttggtcaca atgtagccta ttcaagagaa aaacttacac caatactagc gttctatgtt
    20281 gaagaaaatg tagaagctat tatgaacaga tgcagagaaa tattgttaaa tgaaggtgca
    20341 ggtcatacat tctcaatgca tgtaaatgat gaagaattgg taaaaagatt tgcccttaat
    20401 atgccagtat caagaatact tgtgaatact ccttcagcac ttggaggaat aggtgcatca
    20461 acaaaccttg taccagcact aacacttggt tgtggtgcaa taggtggaag ttcaacttca
    20521 aacaatatag gaccacttga tttaataaat gttaaaagag tagcttatgg aatcaaagaa
    20581 atagaagaac ttggaagaga taaatctcaa gttgaaaaat caatgtttga ctcaatggat
    20641 aaagaagaat taataaatct tcttgttcaa aaactagtca aagaactagc ataactaaaa
    20701 taaacaatga aacttatggc tatcgctata aatttctaaa aatataaact ataaaaaact
    20761 ataaaattaa atttttggag gataagatta tggcaagtgc aaacgcatta ggaatgatag
    20821 aaactaaagg attagtagga gcaatagaag cagcagatgc aatggttaaa gctgcaaatg
    20881 tacaattagt aggaaaagag caagtaggtg gaggattagt tactgtaatg gtaagaggag
    20941 atgttggagc tgttaaagct gctacagatg caggagctgc cgctgccgag agagttggag
    21001 aactaatatc agttcatgtt ataccaagac cacactttga agtagatgct atattaccaa
    21061 aggtatcagc tgagtaatat aagaataatt ataaaattta aattttgatt gaaaaagagg
    21121 ctatctcaaa aatgagtctt actaataaac tcaatgtatt ttgaggtagt cttttttata
    21181 catctaatag gagatgtgat aaaatgggtg ttttaacaga aaacgaagta aaaagaaaaa
    21241 ttggaaaaaa caaaaataat ataactgaat tttatataga aaaaggtcaa ataataactc
    21301 cttctgcaaa atcttattta gctgagagaa gtattgagct taaatatgtt gataaacttt
    21361 cagatgtaaa caatgaacct aaagaagttg taaaagaagt agttaaagag aaaaaatata
    21421 gatatactac tgtatttggc gctaaattgg atgaaaaacc agaacatatg actcaattaa
    21481 atggaaatct tttagttttt aaagaccata aaagaataat attgagagga aaaatagatt
    21541 ctcttgaatc agaaatacta aaggtacaga tactttgtca gaaactagat atgccaaagc
    21601 ttgttaagga attgcaagaa gtattagact ttgtaagaaa tataatgaga tgtgaagttt
    21661 tagatgaaaa gctaggggac tttaaacttt taggattaac tcctcaagaa cttagagaac
    21721 aatctcacta tcctaaaaaa tactttggta taggacatga gttcccaagt tatgaaatgg
    21781 gagaagtagt agtagctata aatagtataa gaagtaatac acgagaggtt gaacttgcag
    21841 cctatgaagc atttaagggt gagtatggaa atgttcacag agaagattta ataaaggcac
    21901 ttaatagatt atcgtcagta ttttggataa tgatatataa ggttagaact aataagtata
    21961 agtagttgga ggacataatg gagaatctat taaatgaaat agtggaagaa gtaataacta
    22021 gagttaaaaa agaagcattt atagaagttg aagcatcagg aagacacgtt cacttaagta
    22081 gagaagatgt cgataaatta tttggagaag gatatactct tacaaaacta aaagacctat
    22141 ctcaaccagg tcaatatgca tgtaaggaaa gagttacaat aactggtcct aaaggaagta
    22201 taaaaaatgt agttgtactt ggaccatgta gaaatgaaac tcaggtagaa atatctttaa
    22261 cagatggttc aactcttgga ctaaaagctc ctataaaaca aagtggagac ttagaaggta
    22321 ctttaagtat taaaatatca acacaatatg gtgaagttga acttgataag gggttaatgg
    22381 ttgctaaaag acatatacac atgactccta aagatgctga aaaattcaat gtatgtgata
    22441 aagagatagt tgaagctaag gtgatgggtc aaagaccttt aacatttgat gatgtagtaa
    22501 taagagtgag tgacagtttt aaaacttata tgcatataga ctatgatgaa gccaatgcat
    22561 gtggttattc aaaaggaact gttgctaaaa taataaagaa agcgtaaatt taacctacta
    22621 tataaattac ctctagggag gatttaatat gaattatgat aaccttgtag atttaataac
    22681 ggaagaaatc tataaaaaaa taaatagtaa tgaaataaaa atatctaata aacctaaagc
    22741 tgtgattgta tttgaacaag ataacaataa atttaactta ttaaaagatg aatttgaaat
    22801 agtagaattt gataaaagta taagggaatg tgaaatagtg atagtttcaa gactttgtat
    22861 gagggggcta tcgaatatag cacttggaaa ttcaacaagt gatgaagaaa gatttatact
    22921 aaaaatgatt atgaagggca aagaagtata tgtcctagat gaaggaattg aatacaaaaa
    22981 atataaggat actgcaccaa aagcattata taagaaattt atgtcatttg aagatgaaat
    23041 atgtaaattt ggtgtagaaa ttataaaaga cttaaatgtt ataactaaaa ataagttaaa
    23101 tataaagaaa gaactagaaa gtactagagg ttctaaaaaa gaagatacaa aagttttaga
    23161 taaagatgtt ttaaatttaa ccagtgtaga aagtgtaaaa atagataatg aatttagttt
    23221 agatttaagg aataaaaaac ttatatcgga agctgactta agaaaaccaa ctataaatgg
    23281 agttaaaaac atattagtta ataaaaaaag tataataaca ccattagctg tagattttat
    23341 gagaatacac catctcaaac taaagaaagt gtaaggaggt aattttatgg atataggaaa
    23401 agttgtaggt aatgtttggg ctacaagaaa agatgaaaaa ttatgtggcc aaaaactttt
    23461 agttgtaaaa attttaaaaa ctaaagacac atacaaggaa ggcttcatgg ttgcagccga
    23521 taatgcagga gcaggaaatg gagacttagt tcttatatcc aaaggttcct cagctagaga
    23581 gtcgattgaa aaatctcatg ttccggtaga tgcaactata gttggagtag tcgattcgtt
    23641 tgaggtgttt gatgaataaa agtataggag ctatagagtt taaaagcatt gctaaaggta
    23701 tagaagtatc caatgaaatg ataaagaaat cttcagttga tgtattgtac ttaaaaagta
    23761 tttgcccagg taaatttcta ataatagtag gtggagaaac ttcttatata aatgaatgtg
    23821 tagattatgg aataaaactt ggagaaggat atattgtaga taacttcgta ataaatgcaa
    23881 tttcacaaga aatattagat ggatttaaaa ataagtatca aaagctagat agtatagtct
    23941 ctataggcgt tgttgaaagt agtaaagtat gcgctggaat aaaaatgctt gataagactt
    24001 taaaatctgg cgacttagta ttagtaaaac ttcaattatc ttttgctata ggtggcaaat
    24061 tagtatatat agtagcagga gatttaagta gtttagaata tgcattaaaa gaaagtgaaa
    24121 atgtagtaag agaaaaagaa gtaatatata agactgtaat accatcagta gacagtcaaa
    24181 taataaaaag tctaataaaa taatgggggg aattatgagt ataaacgaaa ttataatcta
    24241 tgtaatggta gtcttcatgg tacttggagc tatagataaa tgtataggta acaaatttgg
    24301 tcttggagaa caatttgaag aaggtataat ggcaatgggg tcacttgcag ttgcaatggt
    24361 tggtgttata tgtttagcac cagttcttgc tgatgtatta agaccaattg tagtaccagt
    24421 atttaatata cttggagctg acccagctat gtttgctgga agtttattag ctaatgatat
    24481 gggtggtgca ccacttgcac ttgaacttgc gaaagaccct aatgctgggt tatttggtgg
    24541 attaatagta ggggctatga tgggacctac aatagtattt ataataccag ttgcgcttgg
    24601 aataattgaa aaagaagacc aaaaattctt agcaacagga atacttgcag gtataataac
    24661 aatacctatt ggagcattcg ttggtggatt agttgcagga ttcgaggtaa tgatggttct
    24721 tagaaactta gtgccaataa taatatttgc agtagttata gcacttggat tattaaaatt
    24781 cgaaaatgct atgataaaag gattcacata ctttggaaaa ggtgtagtaa tagttataac
    24841 tttaggactt gctgctgcca tagttgaagc tttaacagga atagttgtaa tacctggaat
    24901 gaaaccaatc agtgaaggta tagaaatagt tggagatata gcaatagttt tagcaggagc
    24961 attcccatta gtgtttgtta taacaaaggt atttaataaa ccattaatgg ctttaggaaa
    25021 agttcttgga atgaatgatg tatcagcagc aggccttgtt gcaagtttag ctaactgtat
    25081 accaatgttt ggtatgatga aagatatgga caatagagga aaaataataa atgtagcatt
    25141 ctctgtatca gcagcattcg tatttggaga ccacttagga tttacggcag gatttaattc
    25201 taatatgata acaccaatga tagttgctaa attagttggt ggtataacag cagtattgtt
    25261 agctatggtt atcgcaaata agacactaaa gaaggagaat gtataagatg gatatatcaa
    25321 atatagataa aaatttaatt gaaacattag taagacaaat aatagaagaa aaaataagtg
    25381 gaactaaaga cacagtagat tttgttagaa ataaagacat tagtggaata acttctataa
    25441 agttaccaac tgtaaaagtt agtgaatctg atagattaga tacagggaat ccaagtgatg
    25501 tagtttacac aaaagattta tttacattag aggaaagccc aagattaggt tgtggtatga
    25561 tggaaatgaa agaaacaact tttgattgga ctctaaacta tgatgaaata gattatgtaa
    25621 tagatggaac tttagatata ataatagatg gaagaaaggt ttctgcttct tcaggtgaat
    25681 taatatttat accaaaagga agtaagatac aattttctgt acctgattat gctagattta
    25741 tatatgtaac atatccggca gattgggcat ctcaaaatta ataaaaatca agttaagtta
    25801 ataataatca aataaataat ttaaaataca aactcttaaa taagcacaca tatttaaaaa
    25861 agctatgaat taaaattcat agcttttttt ataaagaatg ttatatttta gacgtatttt
    25921 ttctatattt actgggagac atattatatt ttttattaaa taatctaata aaatgctcta
    25981 catttggata accaatacta ttttactgat aatcttttcc ttatcactaa tcttacattt
    26041 taaaatatta tttttactcg taatattatc ataagaattt gtttcttatt taaaactaat
    26101 tagaattata aagtttattg ccatgttatt aataaaataa atccagttaa atcgagtgct
    26161 agttaaagta ataagaaact tttacaatgg tttcctttat cgattgaaat aaaagttaat
    26221 atggtataat atctaaagag agaatcttat caattagaat atgcatagaa acaagtggct
    26281 taaaaggaga cgttatatgg aagagatagt aaagaaggaa caagcaagta tgaatgatat
    26341 ttttttgtat cttaaagcta aatcaaactc attatataaa tttgtaatat tatataatga
    26401 gtttatcaat aaacctaaag attatggaac tggggaactt ataaacatgg ttgaagtaca
    26461 tatgctaaca gcgattgaag aaaatccagg aataagtcca actcagttag ctaaaatatg
    26521 gaacagaact aaaggtgcaa tatctcaaac tatatctaaa ttggagaaaa agggttatat
    26581 aactaaagaa aagaaagaag gaaatgcaaa aactatatta ctatatccaa ccacatcagg
    26641 agtagagtta agtaaggcac ataaactata tgacaccata gatgttacaa gaacatttga
    26701 atacttaatc aaggagtgta cattagagga gatagatagt ttttataaag taattgattt
    26761 gtttattaat ttattagaag aatctccgca ggaaaataat gattctgaaa aagtgtagct
    26821 gagattgaag ttgaataaag agaaatagat attatagaag tagtacttaa gtacacatta
    26881 tataaaaata taataggtat aggattaaaa agtttagtgg tttaaagttt aaccactaaa
    26941 cttttttaat gtctataatc tactattatt taatttacta aaaataaaaa aactcattat
    27001 attaagataa aaatagaaaa ataactagaa aaatactata atatatcagc ttaatacaga
    27061 aaaaagttaa aaaatttgag aaaaataaaa atgaaaacgt tttatattga catagagaaa
    27121 aagtagtgat accatttaaa tatagtttag tagctaaact atatttaaat tataataact
    27181 attttagggg gatgttatga atttaaaaca attaagtgaa aaatacgaag aatatgtaat
    27241 tcaaatgaga agagaatttc ataagtatcc agaattatct tttaaagaaa taagaacaac
    27301 ccaaagaata agggaagaat tacaaaaaat aggggtccct tttgtatcta ttgaaccaaa
    27361 tatcgtaata gctactatac aaggcaaaaa tgaaggtaag acaattgcaa ttagaggaga
    27421 tatagatgcg cttccaatgc aagaagattc agatgttgaa tacaaatcag aatatgatgg
    27481 aataatgcat tcttgtggac atgatgctca tggagctatg ttactaggac ttgcacatat
    27541 gctaaatgat ataagaaaag atttaaaagg tagagtatta ttagtttttc aagcagctga
    27601 agaagttggt ggtggacaca aggagataat acagtacttt aaggaaaatg gaggaccaga
    27661 tagattaata gctactcatg tttggtccga tattgaagct ggtaaaattt ctgttgaacc
    27721 aggacctcgt atggctggtg ggtctggttg gagaatagac ataacaggta gaggaggtca
    27781 tggttctaga ccagatcaaa gcatagatcc tataaagcca gcttgtgata tagttttaaa
    27841 agtatcttct ataccagtta atcatataag tgtactagaa caatgtgttg tacatgcatg
    27901 tgcaatacat ggaggaacta caataggaaa tgttattcca aacaatgcag aggtaatagg
    27961 tggatataga tattttactg aagaatcagg caaaaaagtg tttgacatta taaagcaaat
    28021 gagtaatggt gttggacaag tttatggagt ggatgtgaag gttactcata ctggaggcat
    28081 aggacctgtt atcaatgatg aagaagcagt taagatggct aaagaaatag tttctaatat
    28141 agatggattg gagttagatt cttatgagcc aatatgtgca tcagattgtt atggtgaaat
    28201 attaaaagag tattctggct tttattgtta tctaggggta ggtaacaaag aaaaaggaat
    28261 aatatatgca caacaccatc ctaagtacaa tatagatgaa gatacattaa agttaggttg
    28321 cgaatttatg gctaaatatg cagttgaatt tttaaataaa aataataact aaaataaatc
    28381 gaattttata aatggggggg atgaaaatgc aaggtgcatt aaaggaaaat aagaaaaaaa
    28441 gtggtggagg atttttagga tggattgaaa aagttgggaa taaaatgcca catcctctag
    28501 cattatttac atatattaca ttgatagtag tagcattatc tgcaatagca agctttttag
    28561 gtttttcagc aacaagtcct gctacaggaa agttaataaa agttatgaac ttaataagtg
    28621 caaaaggttt agtactattt atgcaagagt ttgttaagaa cttccaaaac ttccctgtgc
    28681 ttggagtagt tttagttatg gcagttgcca caggagtttg tgaaaagaca ggattttttt
    28741 caactgccat aaaaatgagt ttatcaaaag ttaaggggaa tgcggttgta tttataatag
    28801 cttttatagg agtatttgct aatcaagctg gtgatgctgc ttttgtatta gtaccaactc
    28861 ttgcaggagc aatattttat ggattaaata gaaatccatt agctggtata ttctgtggat
    28921 ttgcatcagt tggtggtgga tttgcaacag ctgtaattcc tgggggatgg gacgttacgc
    28981 taacgccaat aactgtatca gctgctcaaa ctattcaacc tgcatttgat atgacattat
    29041 tagctagtta ttatgcacta tttgtatctg cgtttatagt agctacagta tcaacaatag
    29101 taacggttaa atttatagaa cctaaattag gtaagtatac tggtaaacca gaaggtatag
    29161 atgataatca aggttttgag gtcacaaaag aacaagcaaa agcagcaaag ttagcaggtt
    29221 ttacagtttt agcttatgta gcgttattaa ttgctttgtg tataccagct aatagttttt
    29281 taagaagtcc agaaggttct ttaatatttg gagcaccact tatgtcttgc ttacagtttt
    29341 ttatagtaat actattcttc ttgccaggga ttgtgtatgg aatgagggtt ggtaaaatca
    29401 agactgtaaa agacttaaat gatatattat gtcaatcaat ggcagcaatg gcaccgttta
    29461 tcgtacttgc aatagttata ggtcaattct taacgctatt ctcagtgtct aatttagcac
    29521 aggtactagc tataaaaggt ggagcattac tcaactcatt gccactacca ccacaagcta
    29581 taatactgct attcttagta ctagtagcgt ttataaatat atttgttata agcggaagta
    29641 caaaatggat gatatttgga ccagtatttg ttccaatgtt aatgcaatta aacatacatc
    29701 cagcatttac tcagtttgta tatagattag gagattctgt aaccaatcat ttatcaccat
    29761 taaatgcatt ctttataata cttttagcaa cagtccaaaa atacgataaa aatgcaggta
    29821 tgggaactat attctcagct atgatacctt acactatagg ttactttgta gtacttgcag
    29881 ttttagtagt agtttggatg acaataggta taccagtagg aataggtgga caagtttggt
    29941 tataaaacat atttaataat tttgggagga aagaaaatgg aaacaactga aataagatac
    30001 aattttgatg aagtaataga tagaagtgga acaaactgtc ttaaatatga tcttaagagt
    30061 caatttatgc cagaatctcc agaagatgca ctaccactat gggtagcaga tatggatttt
    30121 gcatgtgccc ctgagataat taatgcaatg catgaaagaa tagacagaaa aatatttgga
    30181 tatagctctc aacaaactga tgattattat gaagctgtat gtggatggtt taagagaaga
    30241 tttgattgga aaatagaaaa agagaatata ttctttagcc caggggttgt gcctgcgtta
    30301 ggatttttca cacaaatatt aacacaaaag ggtgatggta tcataataga aaagcctgta
    30361 tactatcctt ttgaagataa aataactaat aatgaaagaa aagtagttaa tagtccttta
    30421 aagtttgaag atggtcatta ttctatggat tatgatggat ttgaagaatt agctaaagat
    30481 gaaaataata aggtgtttat attatgtagt cctcataatc cagttggtag agtatggaaa
    30541 aaagaagagc ttagaaaaat agtagacata tgtaagaaat atgatttgtg gataatatct
    30601 gatgaaatac attgtgatat tattcgtaaa ggtgaaaagc atataccatt agaagtagca
    30661 tgtcctgatt ataaagatag aataataact tgtacagctc caagtaagag ttttaactta
    30721 gctggtatgc aattatcaaa tatagtaatc aataataagg aattacaaaa aaaatggatg
    30781 cttgaaacag attctaaatt agctatattc ctacctaatc cattcgcaat agttgctact
    30841 aaagttgctt ataatgattg tgaagattgg atgaatcaag taaatgagta tatagatggt
    30901 aatattaaat atgtagaaga ttttgttaat gagtatatgc ctaaagtaaa gataataaaa
    30961 tctcaaggaa cttacttagt ttggttagac tttagagggt atggattaga tgcaaaacag
    31021 cttgaatatg taatgcttaa caaggcgaag gtattgctag atgaaggata catattcgga
    31081 gaagaaggag caggatttga aagaataaat gttgcttcac ctagaacaat tatagaagag
    31141 tgtatgaata gaataaaatg tgcatttgaa ggattataat agtaatatct aaataatata
    31201 tttgtgtatt ttaaaaaaag actatgctaa aaaaatattt caaagcatag tcttttaaag
    31261 ttttatgatg agtttaaaaa atttcaaaac gttactattc acttatatgt ttatattaga
    31321 aataattcca caatatactc attttctaaa gttgcacttt tatacacctt gcttcaccaa
    31381 tatcatgtgt aaccaaaata gtagacttat tctcattctt aataatacta taaacatcat
    31441 cacaaactaa taatctagtc tgataatcaa gagctgaaaa aggttcatct aaaagtagaa
    31501 tatctggatt tacagaaagt gttctaataa gcgcaactct ttgtctcata cctccagata
    31561 attcttttgg atacatactt ctaaaatccc ataatccata agtctttaaa aggctttcaa
    31621 ctctatctaa agatttttta tcttttttgc cttgaatttc caatccaata gttatattat
    31681 ccattatatt tctccattca agcaaattat ctttttgaaa catatatcca atatttccag
    31741 taatctttat atcaccacta gtcggtttta aaagattagt aagaatattt aatattgtag
    31801 atttaccact tccagaagga ccaagcaaag ttaatatttc tccttctttc aagttaaaat
    31861 tgacatcctt taatacttca agctcgcctt ccttagtgta gaagttcata tttatatttt
    31921 taacttctac tatgttgtca ttcattatgc caccccctat aacttttagc tcgtatatat
    31981 tatgttatta acaatatgac ctatttgtac catttttcat aaatataata taaatatttt
    32041 gtttatttac gtattttctt agtaacttgt acgtataaat tatagataaa taaattataa
    32101 ggtaggggat aaatttgagt aacataataa cgaatttaat agagtcaata tttactaatc
    32161 caataactat attgttttta cttgcaataa ctgtgtattc tgtttttaaa ataatagaaa
    32221 ataacagagt atattcattt gtaagttcaa aattagacga aatcaataga gactataaga
    32281 caagtgagtt ttataaaaag gtaagggatg actactatac atatagtaaa gaaaatcctt
    32341 atgcagacgt aaatataact tcatttatag aggaagttgt atctgattta aagcataata
    32401 atttgccatt gttagaaaaa attagaagta taaaaaattc atcttcaata agtatattac
    32461 ttggggtttt aggtacgttt gttggtcttt ctacaatgct tttatgtgtt gatactaaag
    32521 acattataaa ttcacttcct tcaactatta gtagtatgca aactgcattt acaactagta
    32581 tatttggagt tgtattttct ctaattatag gttactttac taagataaaa gattgtgaac
    32641 atgttttgat acaaataatg ttaaaatctg aaaatttact aacatctgaa ataactcatt
    32701 ttaagtcaga aagaatggat ttaaaagtag aagaggtaaa aaatacaata aaacaaataa
    32761 gtaagtctat agaagctatt gaaaaatttg ataaaatatc taaagattta aatgacttta
    32821 atgatgaatt tatcagtggt atagaagctt taaaaagcct tttagaaggt tctcaaagtt
    32881 ctattaagac atttgaccag agcgtaagaa aattagataa acagtttaat attttaaata
    32941 taaaatttgt aaagcttttt gataaatatg ataatcaaga taatataaat aaagagattt
    33001 tatttgatat aaaagaatct tcaaaaaata tatacaatgc cactgaaagc caattcaaaa
    33061 ttagagatta tataaaaaat ataaatgcag gatttgctct atatgaaaga agtgctcaag
    33121 atttattaac taaattaatg acccatgaaa ataaaatatc tcaaaatcaa aaaatccttt
    33181 tagatgaaaa gtttacctta gatgactcaa taaaaaatct ctcaagtatt attgaaaact
    33241 tttctaatga cctacaagtt aaactggata tgatgtttga aaattcttta gatattcaag
    33301 ataagttaga tgttatgttt aataactctt ttatgaatga tgaagttcct cttgatagtg
    33361 aagaattatt taaggatgat gttaataatg tatttaatcc ttttagtgaa gaaatttatg
    33421 agatagagga taaagaaata aaggtcatag gtgaggatga gttaaatgaa taataaatat
    33481 agaagaactg ttaacagaga atttgaaaag agtagctttt ggccaacatt tacagattta
    33541 ttatctactg tattgatggt tgtgattctt atcttattta gctcagaaag tatttcaggt
    33601 tctgttgaac aagatttagc taagaatgtc aatgcttctg ttgaagaaac ttttaaaaaa
    33661 agtggaatac ctgtgaaggt agataaatct aatgggcaag taacttttgg tgaaaaaacc
    33721 atgtttgatg tagatagtga tgttttaaaa ccagaggcta aagaaatgct aaaaatgttt
    33781 gttccaaagt acatagagac tatttataaa gattatggtg actatatatc caaaatagtt
    33841 atagaaggtc atacagatga tgtgggaagt tatatataca acttagattt atcgcaaaga
    33901 agagcatata gcgttgctaa atttatagtg ggagatgaaa taggtgacta taaatacaaa
    33961 gataaagtga ctaagcatat tattgcaata ggaaggtcaa aagctgaact catcaaaaat
    34021 ggtgacaata gcgtgaatag agatgcatct agaagagtag aactaaaata cgaaataaat
    34081 attaatcaaa ataaataatt aaatttttat aaaaaaaaag aaggcagttg gtgtgagctt
    34141 taagccttct ttaaggaaaa agtttattta tttttgttga tacttaacat attatagttt
    34201 ttagaagcag tttgcaatat attaaacgta aatggtcgtt taaaatgcat aactggatac
    34261 tactgatgta aaatatttca ttttatataa tataaaaatt atacagtgtc aattttctat
    34321 attattgtac tataatctat tatttaaaat attatgtaaa aaaatatcat aaaaacgcat
    34381 atcatgttat aattactaga gtattatgaa tatggaggcg tagtttgtga tagaaataag
    34441 tataaaaaat aaaaaaaatt ctaaaattaa aactttgata cttttattga tagtatctat
    34501 atcgatttat ttaactggtt gtactacatc agataatgga aataatcaat caaataataa
    34561 tgattctttg tctagtagta tatcaagtga taagaatgta aaaattcatt ttattgacac
    34621 tggaaatagt gatgcaatat tgattcaaga tggtaaaact tttacattga tagatggggg
    34681 agataatgat gatgagggat taatggtaga ttacctaaac aatcaaggtg taaaagatat
    34741 taaatatctt atagctaccc attctcatgc agaccattta ggtggacttg attctgttgt
    34801 caaaaacttt aatatagaaa atgtttttgt atcgaatggt tcagcagaaa caaagagtta
    34861 tagggacttt ataaatgctt tagcaaataa gggtttatcc ccaagtgtac cgcttgaaaa
    34921 taataagttc tatttagaag attcttattt tgaagttcta aatacaaatg ggggagatac
    34981 tactaatgaa caatctctag tccttgtata tactaatggg aatgataaag ttttatttac
    35041 aggtgatgct gaagaaggta ccgaaaaaga aatactacct aagcttgaga aagtagacct
    35101 tttaaaagta gctcatcatg gttctcgtag tagttcatct caagagtttt tagataaagt
    35161 aaatcctgaa tatgcggttc ttttagtagg caagggtaat tcatatggtc atccacatca
    35221 ggagacaatg aataaattaa aaaaaatggg agtaaaagtg catagaagtg atgagtgttc
    35281 agatataata tttgaatcaa caggtgatgg agtatttaca agttgtaaag atggaagtta
    35341 taataaaggc gttagagaag atggaaactc tggaaaatca aacagtaata caagtaaaaa
    35401 tgattctttt aaaaataaac aaaatacatc caattcagaa gaaatagtgt attttactcc
    35461 aaaaggtaag agctatcact ctacaaaaaa ttgtagtggg ctttcaagaa gtaaaaaaat
    35521 attatcaggc acaattgcag aaagtaagaa aaatgaccca tgtgatatat gttatggaaa
    35581 ataacaaaaa aacaaccaat ttatctataa agcttctgaa ctttaaatca tttaaaaaat
    35641 aaaataaatg cataaaaaaa taagctataa tcaatagata acacatttta tctatacgaa
    35701 tagcttattt ttttaagtat tttttaatag ctatttaata actctaaata caccagttac
    35761 aatacctaca atttcaactt ctgagtcatt gagcataatt ggttccataa aatcattttc
    35821 tggctgaagt cttattaaat ttccttcttt aaaaaacctc ttaaccgtag cagattcacc
    35881 atttataagt gcaactacta tctgagaatt taaggctgta ttttttttgt ctactacaac
    35941 gtaatcttca tctaaaattc ctgcattaat catactttcg ccttttactc ttaatacaaa
    36001 attgttactg ccctttacta aactggcagg gaatggaatg tattcctcta tgttttgttg
    36061 agctaaaatg ggttctccag ctgttatttg acctactaaa ggaagctcta ttatttcttg
    36121 attagctcca gaaacatcat ttactttact acgttctaaa acttcaatag ctcttggttt
    36181 agtagggtcc tttcttatgt atcccagttt ttcaagttta tttagatgag agtgcacagt
    36241 tgaagtagat cttaacccta cagcagtaca tatttctctt acagcaggtg gataaccttt
    36301 taatatgatt tgagacttta taaattccaa tatcaagact tgcttttcag ttagatctaa
    36361 atacataaca tcactcattt ccaaacatat tttgttttta tttccataca ctttatattt
    36421 ttaattataa cataatccat ttatatctgc aaacatttgt tcctgatttt taatcgtcct
    36481 caatccattc aaaattcttg actatatcac gttttacatt tttcttatga gctgcataaa
    36541 gctgagtagt tgtgacatta tcatggtcaa gtaagttttg aacatcatat atagaaaatc
    36601 ctgtttggat tagagaagtt gcagccgttg ctcttaattt atggggacta tatccattgt
    36661 ctctagtagt atcaagtgga atagatgtat atttttttac cagtgttctt atagcctttg
    36721 ctgtaattct cttattttga agagacaaaa ataaagcatc tttttcttca tcatttaata
    36781 gttcatctct tgttctttcg ttttgcaaat aatcttttat tacatgttca caagtatgat
    36841 ttataggcat taaaacttct ttcccacgtt ttctatatat tttaaattca cctcttgaaa
    36901 aattaaaaga ggagatattg agttctctaa gctcatttaa tctaagcccg tatgtaacaa
    36961 ataatgctag tatagctttg tcacgtaatt tagtttttct ccaatatacc ttttctttct
    37021 ctgtaagccc ttcacctgtt tctactgatt caagcatctt agcaacttca tctatctcaa
    37081 ggcgcttaat agcatctggc tgaggtttag gaagttttat tggattgaat ccatcagtta
    37141 tattattatc tatctgagag tttctgtata agaatttaaa tagagtagag atggatgatt
    37201 tttttcttgc taaagctcta ttgttatttt caaaaatcaa tgtgtttttt tctgtatttt
    37261 tgtaatatct actgcaataa tcacctaaga ataaatttat atctcttgat tttattgtat
    37321 taaaatcatc ttctgtaatg tcttttacac aatctgcatt tgttaattct ttggtttcaa
    37381 tcaaatatga acaaaaaaaa ttaatatctt ctaaataagc aagtcgtgta gaaactgcaa
    37441 cagaaccttt taaatatata aaataatcct tcataaatac aggaagttta ttttccaaaa
    37501 gtagacattt ttctatgagt tcattttctt tgataacaat attatcaggt ttatctgatt
    37561 tattatgtac ttttataatt ttcttatcat tattcaaaat taaacctcct aaaactaaaa
    37621 tataaatctt ttatattatt ttttgatatt tatactaagt gagtataaat atttaattta
    37681 atcatttttt acatttttaa gagattagca gtgctttaga tgaaattatc actaactatt
    37741 ccctaaatcc acatttaaag actagttgaa tttaagtact cttttttatt tctcaatata
    37801 tttatataaa tatattgaga aattttttat gctctactag aatctttttt a
//
LOCUS       DAYASV010000023        37622 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYASV010000023 DAYASV010000000
VERSION     DAYASV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37622
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.023"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(130..324)
                     /locus_tag="ACGI68_001209"
     CDS             complement(130..324)
                     /locus_tag="ACGI68_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137655.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI68_001210"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="ACGI68_001210"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFH0137656.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI68_001211"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="ACGI68_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFH0137657.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI68_001212"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="ACGI68_001212"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFH0137658.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRATEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="ACGI68_001213"
     CDS             complement(3870..4640)
                     /locus_tag="ACGI68_001213"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HFH0137659.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="ACGI68_001214"
     CDS             complement(4659..5249)
                     /locus_tag="ACGI68_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0137660.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTATSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5432..6400)
                     /locus_tag="ACGI68_001215"
     CDS             complement(5432..6400)
                     /locus_tag="ACGI68_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HFH0137661.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6393..7370)
                     /locus_tag="ACGI68_001216"
     CDS             complement(6393..7370)
                     /locus_tag="ACGI68_001216"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFH0137662.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI68_001217"
     CDS             complement(7685..9655)
                     /gene="ftsH"
                     /locus_tag="ACGI68_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFH0137663.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIIVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI68_001218"
     CDS             complement(9689..11077)
                     /gene="tilS"
                     /locus_tag="ACGI68_001218"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFH0137664.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDGEGIICVGDYKISENYKIDES
                     TKEVLKINFNKL"
     gene            complement(11260..11688)
                     /locus_tag="ACGI68_001219"
     CDS             complement(11260..11688)
                     /locus_tag="ACGI68_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFH0137665.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI68_001220"
     CDS             complement(11705..12511)
                     /gene="murI"
                     /locus_tag="ACGI68_001220"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFH0137666.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12713..13198)
                     /locus_tag="ACGI68_001221"
     CDS             complement(12713..13198)
                     /locus_tag="ACGI68_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HFH0137667.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI68_001222"
     CDS             complement(13249..13908)
                     /gene="spoIIR"
                     /locus_tag="ACGI68_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HFH0137668.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14053..14733)
                     /locus_tag="ACGI68_001223"
     CDS             complement(14053..14733)
                     /locus_tag="ACGI68_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0137669.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI68_001224"
     CDS             complement(14717..15607)
                     /gene="ispE"
                     /locus_tag="ACGI68_001224"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFH0137670.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15725..17275)
                     /locus_tag="ACGI68_001225"
     CDS             complement(15725..17275)
                     /locus_tag="ACGI68_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HFH0137671.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVIQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVECIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17369..17635)
                     /locus_tag="ACGI68_001226"
     CDS             complement(17369..17635)
                     /locus_tag="ACGI68_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFH0137672.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI68_001227"
     CDS             complement(17783..18643)
                     /gene="yabG"
                     /locus_tag="ACGI68_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HFH0137673.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18727..19665)
                     /locus_tag="ACGI68_001228"
     CDS             complement(18727..19665)
                     /locus_tag="ACGI68_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HFH0137674.1"
                     /translation="MKFLDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19775..19936)
                     /locus_tag="ACGI68_001229"
     CDS             complement(19775..19936)
                     /locus_tag="ACGI68_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137675.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19946..20443)
                     /locus_tag="ACGI68_001230"
     CDS             complement(19946..20443)
                     /locus_tag="ACGI68_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HFH0137676.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20558..21268)
                     /locus_tag="ACGI68_001231"
     CDS             complement(20558..21268)
                     /locus_tag="ACGI68_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HFH0137677.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21444..21974)
                     /locus_tag="ACGI68_001232"
     CDS             complement(21444..21974)
                     /locus_tag="ACGI68_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137678.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21991..22221)
                     /locus_tag="ACGI68_001233"
     CDS             complement(21991..22221)
                     /locus_tag="ACGI68_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137679.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMAVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22236..23630)
                     /locus_tag="ACGI68_001234"
     CDS             complement(22236..23630)
                     /locus_tag="ACGI68_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFH0137680.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23756..25009)
                     /locus_tag="ACGI68_001235"
     CDS             complement(23756..25009)
                     /locus_tag="ACGI68_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFH0137681.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTIPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25060..26214)
                     /locus_tag="ACGI68_001236"
     CDS             complement(25060..26214)
                     /locus_tag="ACGI68_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0137682.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGISGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27343)
                     /locus_tag="ACGI68_001237"
     CDS             complement(26774..27343)
                     /locus_tag="ACGI68_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0137683.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27546..27773
                     /locus_tag="ACGI68_001238"
     CDS             27546..27773
                     /locus_tag="ACGI68_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0137684.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27992..28735)
                     /locus_tag="ACGI68_001239"
     CDS             complement(27992..28735)
                     /locus_tag="ACGI68_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0137685.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28903..29775
                     /locus_tag="ACGI68_001240"
     CDS             28903..29775
                     /locus_tag="ACGI68_001240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0137686.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29775..30440
                     /locus_tag="ACGI68_001241"
     CDS             29775..30440
                     /locus_tag="ACGI68_001241"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0137687.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30461..31318
                     /locus_tag="ACGI68_001242"
     CDS             30461..31318
                     /locus_tag="ACGI68_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0137688.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31474..33225)
                     /locus_tag="ACGI68_001243"
     CDS             complement(31474..33225)
                     /locus_tag="ACGI68_001243"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137689.1"
                     /translation="MFLYSVGGIVLAIIMLGSIFLIYNSFNMSLNERTHQFGILSSVG
                     ATAKQLRNSVLFEGICIGAIGIPIGVAIGIVSIGLVISVISKNFANILYVNVPLTLTL
                     SIPAIIVAAVVSMVTILISAYIPARKAANTPVMECIRQTNEVKIESKSVKTSKLTQRV
                     YGLEGVLAMKNFKRNKKRYRSIVLSLVLSIVLFISTSALVADLKQMSNQRSEVTDQDI
                     IFSTQGMKDNEMLKLYDKLKTANGVYEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSM
                     SEMLELPVDMQFLDDRTYLNIIKSLGLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSS
                     SMNVSLTPETNGKLEIPERQNVNISFVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKD
                     KFEIPGVSTGIESLSFQSKNPTQSVAEMEQIIESEGVTNEYNLYNMYGMMDENRNYIF
                     IANVFAYTFIIMISLISIANVFNTISTNIKLRRRELAMLRSVGMSEHDFQKMMNFECA
                     FYGMRALFFGLPIAVISSWLIHKVMVTDDIDFVIPWFSIGISIFSVLFIVFITMLYTI
                     NKIKKENIIDALRDDMA"
     gene            33188..33313
                     /locus_tag="ACGI68_001244"
     CDS             33188..33313
                     /locus_tag="ACGI68_001244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137690.1"
                     /translation="MASTMPPTEYKNILNKLLSETPMNRNTLSLKMEAWSLVLLV"
     gene            complement(33472..34020)
                     /locus_tag="ACGI68_001245"
                     /pseudo
     CDS             complement(33472..34020)
                     /locus_tag="ACGI68_001245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34020..34706)
                     /locus_tag="ACGI68_001246"
     CDS             complement(34020..34706)
                     /locus_tag="ACGI68_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137691.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34789..35778)
                     /locus_tag="ACGI68_001247"
     CDS             complement(34789..35778)
                     /locus_tag="ACGI68_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0137692.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35765..36451)
                     /locus_tag="ACGI68_001248"
     CDS             complement(35765..36451)
                     /locus_tag="ACGI68_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137693.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36448..36570)
                     /locus_tag="ACGI68_001249"
     CDS             complement(36448..36570)
                     /locus_tag="ACGI68_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137694.1"
                     /translation="MDYIRTKETTENCVMISKIVVYYCSAGIYKTNKIKEDESK"
     gene            complement(36951..>37622)
                     /locus_tag="ACGI68_001250"
     CDS             complement(36951..>37622)
                     /locus_tag="ACGI68_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFH0137695.1"
                     /translation="YISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVP
                     VFSMSKLSKVEVSLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVN
                     NHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGIEADIVNRIEEPRPNILDAIRNK
                     QVDIVINTPTKGNDSTRDGFKMRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKV
                     YNIAE"
BASE COUNT        12013 a         6986 c         4214 g        14409 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt ggttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attccacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttctcaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat ggtgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgactcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgttgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta atccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttatcaggat tccatattca tcagacgttt tttccctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttgc tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttc ttataactat gagtcaagct
     5341 accattgtag gatgcccgct ctaacaagaa taattttatc aagatgagtt gccattgtca
     5401 acaatttgac aagaaaaata tgctaatttc atcatatttc tcgttttttt actatatctt
     5461 tatatgccat tatattttct acactatccg ctttttttac tgcatttatt atactttttt
     5521 ccacaacctc tacagccaat gaacctacta atgttatatc tgcttctatt tctcctgttg
     5581 atattgtgaa tattgtatct ccatcatgag gtgtatggat aggaaaaata gacttagcat
     5641 atccatcatg tcccatttga gatatctttt tacatccagc tttggttaac ttagcatttg
     5701 ttgcaattat cccaattgta gtattatcta cactaaatcc acctttactt tcaccttgtt
     5761 tcattaattt atatgtatca attgtttctg tctttttatc atttaaagca ccagctataa
     5821 ccttaccatt ttcataaaca tctccaagag catttacagc aattaatgca gatacaatta
     5881 gaccattatc taattttata gaatagctac ctattccgcc tttcatagca tattcctgac
     5941 ctcttatctt tccaacagtg gctccacaac ctgctccata atttccttga tttaaaatat
     6001 cagtatttgc aacttcacaa gctctataac ccatctttaa atctggtcta catttaaagc
     6061 tccctactcc taaatcaaat aaaactgctc ctacaactat aggcacttta cctacaccta
     6121 catcaaagcc aattcctttt tcttctaagt atctacttac accacaagtg gattcaagac
     6181 catatgcaga acctccagcc aaaactactg catgaacttt ttgaaccata tttacagggt
     6241 ctaataaatc ggtttctcta gtacctggag cagagcccct tacatcaaca ccgcaagtag
     6301 ctccttcttc acatattact actgtgcacc cagtaagacc atttctatcc tcaacctgcc
     6361 caacctttaa gccttctata tccaaaatat tattatacat atcctttttc tcctcttata
     6421 gaaacttcac ctgaaataat ttcttttata cttccatcac ttctttgaac taataaattt
     6481 ccctcttcat ttatgtctaa acatttcact agttctctat tatctccttt taatacatat
     6541 atatttttat ctataattgc agaataatct ctacatattt ttaaggtttt ttctttggaa
     6601 ttatttacag tataatctaa atataatttt tcaaattcag ttaatacatt cctaacaata
     6661 tctactctgg ataacatata tccttcttta tacaatgatg tggctttgtc agctatttct
     6721 tgttcaaaat ccattatttt aacatttatt ccaattccaa gaacaacata atttgttctt
     6781 tcaatttcag cagataactc tgttaatata ccacatattt tttttccatt taaaataaca
     6841 tcatttggcc attttattaa tgtatttgct cctagattgt ttaaagcttt cgttacactt
     6901 gctcctgcta ttaaagttat aaatggggct ttgaatggtt ccatatctgg ttttaatata
     6961 atactcatcc agattccttc atgaggtttt gaatcccatc ttcttcccaa tcgtcctttc
     7021 cccatatctt gatgttcact taaaatcaca gtcccatcta cactatttat agctatcctt
     7081 ttagcatgtt catttgttga atctatagtg tcaagatgtt caatattttt tccaataaac
     7141 tctgtatcta atttatagta aatattctgt gggcttaata aatcagttgg attttccgct
     7201 aatcgatacc cttttttatt tacagattct atattatatc cctcttcttt taaggctttc
     7261 atatgtttcc atatagctgc acgtgacacc cctaattgtt ttgataattc ttctcctgaa
     7321 ataaattcag atttattatt taatataatc tctattattt tatctctcat atttcacctc
     7381 taatatgaca taatttcatg acttattttc ttctaataaa taattataca ttattaatat
     7441 acttttaata ctattaattt taaatttcct ttttaattat tataatggtt gttttatatt
     7501 ttatattgat gatttatata attttacatt ttatttaaat aattttaaaa taataaaaaa
     7561 actgaaagta tcaattaaat taatacatgc aaatataatt tgcttattaa tttaatatta
     7621 tactttcagt tttagtattt aatccacatc agataataat cttaaaatga ccaaggtctt
     7681 aaaactattt atctttatca gatttatctt ctaaattttt atttatatca ataacattgt
     7741 ttctttcttc ttcatctttt tctaatttta tagttaattg ttcttcaact tctttagaag
     7801 aattttcctc tgtaactgca ttttcaattt cattcagagg taactcttta ttaaatgctt
     7861 ttacaaattg ttctgcatct aaagtttcat atataagtaa agcttgtgct acatactcta
     7921 atctatccat attttcttgt aatatacttc ttgtcttatc atatgcacca tcaactatac
     7981 gctttgtttc ttggtctatc tcaaaagcaa cttcttcaga ataatttctc ttacttgaaa
     8041 aactattacc taggaatact tcatcatcac tgtcaaatga cattggacca agtttagaac
     8101 tcattccata ctttgttacc atacttcttg cagtagctgt aactctttct aagtcatttg
     8161 aagcacctgt agatacatcc ttaagtacta gctcttctgc tactctacca ccaagtaaaa
     8221 ctataatatt ttctttcatt tcattttttg tagcatagaa cttatcttca acaggaagtt
     8281 gcattgtaaa ccctccagct cttcctcttg gaactattgt aacttgatga acaggactta
     8341 cttcttcaag tacatgggca caaactgcat gccctccttc atggtatgct gtaagtcttc
     8401 tttctttctc acttattact ttcgattttt tagccacacc tgctataacc tttgttatag
     8461 cttcttctat agtttccatc ttaattttct tttctctttt tctagctgtt aatattgcag
     8521 cctcattcat taagttctct atatcagcag gagtaaatcc tggtgttctt ctagccaaga
     8581 catccatttt tacatcatca cttaatggtt tatttcttga gtgaacttta aatatagcct
     8641 ctctaccttt tacatctggt gtaccaacaa caacttgtct gtcaaatctt cctggtctaa
     8701 gtaaagctgg gtcaagtata tctggtctat ttgtagctgc catgattatt ataccttgat
     8761 ttaccccaaa tccatccatt tcaactagaa gttgattaag agtttgctct ctttcatcat
     8821 gacctccacc aagacctgca cctctttttc taccaactgc atcaatttca tctatgaata
     8881 tgatagctgg agcacttttc ttagcctgtt caaataaatc tcttactctt gaagcaccaa
     8941 ccccaacaaa catctcaacg aaatcagaac cacttatact gaaaaaggga actcctgctt
     9001 ctcctgcaac tgctcttgat agatatgttt taccagttcc aggaggtcct accataagca
     9061 ttccttttgg tattcttgca cccaattcaa tatatttctt aggattcttt aagaaatcta
     9121 ctacctcctg caaatcttct ttttcctcat ctagtccagc tacatcttta aatgtaactc
     9181 tagttttttc atcatcttta tgaaccttag cttttgattt gccaaaactc ataacctttc
     9241 cgcctccacc ttgagactga ttcataaaca cgaaccaaat aatcaccatg aagaatatta
     9301 acagtaatgt aggcaacatt tcgacaaacc aaggtgtaga tggcttagct tcaccaccaa
     9361 aagttagttt cccagcctta gcttggtcta acacctcatc tgcaagttta tttcccatca
     9421 cttctgttgg aacatatgac ttaaactttg tatttgtatc ttttatagta ccttctacag
     9481 aagtttgatt tacaaagtat aatcttgata tattctcatc tgttaattct ctatatactt
     9541 cagagaattt taaatctttt attttttctg taggcttacc tgagaattga actataccta
     9601 ctataattat aaaaacaagt agataaaaac ctgccccttt tagcaactta ttcaatatag
     9661 aagccctcct ttcatacatt gctttgccct ataatttgtt aaaattaatt tttaatactt
     9721 cttttgtgct ttcatcaatt ttataatttt cactgatttt ataatctcca acacatatta
     9781 ttccttcacc atctgtgatt ataggaattt tacatctttc ttctcttggg atttttaaat
     9841 ctatgaataa atctttcact tttttacttc ccatagcaag ctttatctta tcaccttgcc
     9901 ttctgcttct tattactata ccccctttta ccttattaaa atcaaaccct ttagatgaat
     9961 tgtctaattt catactctta tacctatcta tgctcattac ttgggtctca ataatttgtt
    10021 ttaattcttt tattttaata aacccattac taggtacatt gtaataaaat tcaatttcct
    10081 cattaacaat ttcctcattc gttaatatta tactgtcttt ttttctataa acaaatatat
    10141 ttcttggaag agttaacatt ttatttactt ttgaattatc ttctaaagac attatatctt
    10201 ctatatgtct ttggtcaaca aaattagtat ctcctagtat atgttttatg gaatttctaa
    10261 gaactctaac tttgatagca tcatgtaagc aaacaaaatc gtctaaattt atctctacaa
    10321 agcctttttc ttttatattt gaaacttctc taaatttagc ttctgcctct ttctcaatat
    10381 aatcgttatc actttttaaa ctattgctca tcctcactat agattctatt acatttgaat
    10441 taaaattatc cttcatatat ggtaaaaggt ctaaccttat cttatttcta gtgtatatat
    10501 tttctaaatt tgttttatct atcctcggat ttaaattata agcttcacaa tactcctcta
    10561 tctcatttct ttccacgtct aatatgggtc taattatgca attatctcta atataatcaa
    10621 ttccctttaa gccttttaaa cctgttcctc tcatgatacg catcataacc gtttcggctt
    10681 ggtcattgag attatgacct attgctattt tattggcttt aatcttatcc ttaatttcat
    10741 aaaacatttc atatcttagc ttccttgctc cttcttctaa tgataatccc tcattttcac
    10801 aatattttgg tacattaata gactttacaa agaatataat tcccatatcc tcacaaagtt
    10861 tagatacata taatgcatct ttttgagctt ctatccctct tatttgatgg tttaaatgtg
    10921 ctgcatatat ttctatatta aaatcttttt ttaatctatt tagtacatgc aataaacaaa
    10981 ctgaatcagg tcctccagaa agacctaaaa ctattttatc gcctttttgt attaaattat
    11041 gtttatttat agtacttagt accttatcaa atatcatatt tatccctctt acacttagtt
    11101 ttattcactt ttaagtatac tttctataaa aaaacgctaa acccttgtat caattttaag
    11161 aaattaaagt ttaatataaa tataataata ccaaataaat taaataaaag gtagtttttt
    11221 cactaccttt ataaaatttc tggaaaatag ccatagttat tatttagtac ctatgtatat
    11281 atctatttta ttcctaccta aaaataaatc ttgtaccttt atattatagt ctattttcaa
    11341 ttttatgtgt tccccttctt gtatatttat atctaattct cttgtatcaa tatcaattaa
    11401 taaagtacct tgtggggtca catactttgt agtactactt tctccacatt taaacagcat
    11461 tgttgagtta atagcaccaa actttttgat actaacttga tttttaagta tctttatagt
    11521 attagttatt tctatatctt cttctttttc cttgtaaata acatatattc catcattttt
    11581 ataaaaaatt ttaccaaagg cagtcatctc tatggtgtcc atatttcctt tttcatcata
    11641 ttgtctagta tttatactta tttttacact caacgatttt tcctccatag attcttcata
    11701 taaactagta tttttgtatt tctacttttt gaatcttatc aatttttttc tttaagaact
    11761 ctcttgctat atctgaaaat ttttcaggta tatctgatac ataatattga taagttccat
    11821 gaatttctgt attgtttata atattttgca cctctaaaat cttttttaaa tcctttgcag
    11881 tctctttagc tggattaaca agttttatat gctctccaac ttcttcacct attgttctct
    11941 ttaaaatagg ataatgagta catcctaaca ctaaagaatc tatatttttt tctttcaatt
    12001 cttgtaaata tatctttgct gtcagtttag ccacttctgt atttgcccat ccttcttcaa
    12061 caataggcac aaataaagga catgctttat taacaatttc tatactctca tctattttag
    12121 atatttccaa gttgtatgct tttgaactta tagttccttc agttcctatt attcctacaa
    12181 ttttgttttt tgtagaactt acagctgttc tagctcctgc ttctataact ccaattatcg
    12241 gtatatcata tttttcttga gcttctttta agcttcttgc agtagcagtg ttacatgcta
    12301 taactatagc ttttacacct tttgttttta aaaagtttat agcttgaaaa gtatatttaa
    12361 ttatagtttc tttggatctt gaaccatatg gtattctggc agtatctcca aagtatatta
    12421 tatcctcatt tggtaatatc ttcattatct cttttagaac tgttaatcca cctaatcccg
    12481 aatcaaaaac tcctattggt ttattgttca tctttataca acacctactt tacatttttg
    12541 tgtttaacaa caacttattt atcgctaaaa tttatattta tattcataat taatatatta
    12601 ttacctaaat gattattata gtttatttta cacaaaaaat aaaggagcta tcttaaatta
    12661 gaaaataatt tctacaaaaa gacaactcct ttcactactt aaaaatttaa atttattttg
    12721 caaacacatg atttcctatt ctcatataag gattcaatct atttatccat ttgcaagtgg
    12781 ctatttctgg attccaaaaa tatactgctt tattagttgg gtcagttcca taaagagctt
    12841 cctgagctgc tttacgtgct gagtctgttg gtgccatctt tattgaacca tcaagcacaa
    12901 ctgagaaagc atttttttga tatatgactt cttctattgt gtctggaaat ctagggtcca
    12961 atactctatt tattactaca gctgctacag caacctgtcc ctcatagctt tctcctctag
    13021 cttctcctgc aaccaactta gataataaca tcaagtcatt atcagataaa tttattactt
    13081 ctttctttgc tatatttttt tcttgtttgt ttactttctt aatattttct gttggttcat
    13141 atgcaaatac ctctttaata ggcataaata taaaagtcaa agctaatagt gtagccatca
    13201 gtttactcca cctgtccatg attaaaacca ctccttacat tcttattttt aaaatttttt
    13261 ctctaaatcc ttgactactt caagtacttt aaacttaatt tttactctac ttgttttttt
    13321 tggtgtatct tgttttatta acttatattc tttatcagtt aaaacttcct tcaacttatc
    13381 atctgtagat ttatctatca ctccattaga ttcatcaaca aaacatagtg gagggaacat
    13441 aacacaccac cagttttttc cttcaccttt cccaataata atctttaatg ctttatattc
    13501 accagctgga agaactatat tagaatattg tttagtaggg aaattgctat attgtatacc
    13561 tacctttaca ctgtaattat atccattttt aagaataata tttttactta tttcttccag
    13621 actactatat tcctttttta taatagcttc actttctttt atactttttg aattttgaag
    13681 ctttggctgt aaatatttta ttacttcatc tctaactttt agtttaagtt cttggtcttc
    13741 atctgtattg ctgtttgcta taacatgaaa tcttattaat ttatctttat aatcttttga
    13801 tatattgtct acttttttta cctcaccatt tattactatt gtcattattg atatgactga
    13861 tataagactt aatattaata gtcccaatct aatcttaatt tttctcattg aaaattcccc
    13921 ctcaagataa atttatatca tcaattatgt ccaattaaat ttaatttatt cataatattt
    13981 tttaatacta ataaaaaaag ctatcttaaa atgattacaa tcattttaaa atagcttttt
    14041 aaaccttata ttttataagt ctttactaat catagcctta tctatcataa actgctctgc
    14101 tttttctata tgctctgtag catacttctt ggatagctca ctatcacctt taattatagc
    14161 atcaagtaaa tatttatgct cttcaacaat attatgtgat gtatcataag cagacatata
    14221 ggtaactcta aatctatata cttgttccca caatgagttt attaattgga ttaatttttt
    14281 attattagtt gctttaaata tacattcatg aaattctaca tcaagtttta ataacgcatc
    14341 tacatcggat tccaaagtgc tttttttaaa ttcttctgat atatctttaa gctttttaat
    14401 atcttcatca ctaattcttt cagcggcaag atatgctgct aagccttcta ggcttgctct
    14461 tacttctaat acatccataa tatcctttaa tgacatattt gccacatacg caccttttct
    14521 aggcaacata acgactaaac cttctaattc aagctttctt attgcctctc tcactggagt
    14581 cctacttact cctaattgtt ctgctagttg cacttccatt agcctttgac caggctttaa
    14641 ttttccttct aagattgctt ctcttaaatt ctcaaagact acatctctta atggcttgta
    14701 attatccaag ttcagtttag ttaaattctc cacaaatttc aacccctttt tgactgctat
    14761 taacaacata cacctgttta tattttttta ataattcttt ttttccaatt agagcatctt
    14821 ctttattttt aaatagtcca aaaactgtag gaccacttcc actcatcata gaacctaaag
    14881 cattattttt catcattact tgttttatat cacttatttc tttgtgttta cctatagtaa
    14941 cattttcaag aatatttacc atgctttctg atacagcttt tatatcttca gattttaaac
    15001 actctatcaa atatttatta tttggtcttt ttttaatatt attcaaatct aatccttgat
    15061 aaacttcttt agtagaaacg aataagtctg gtttgcatat caatatattt gtatcacatg
    15121 gtaaaccttt gatatttgtt agcttttctc caataccttg agctaaggca ggtcttcctg
    15181 atatacaaaa aggaacatct gcgcccaaat taagtcctat ttcttttaat tcatcttcag
    15241 ataatctaag ttcccatagg tgatttaaac ctactaatac tgcggctgca ttagaacttc
    15301 ctccagccat ccctgctgct acaggaatat ttttttctat gaatatttct acaccttttt
    15361 ttatataaaa tttattttta agtattttgg cagctttata aacaatatta tcttcatcta
    15421 aaggaatatc taaactagtg ctttttacct ttatttcatc ttcgtctaat tcttttatct
    15481 ttactatatc gtataaatct atcgtttgca ttatcatttc aacaaaatga taaccgtctt
    15541 gcctttttcc caagacatca atggataaat ttatttttgc tctactcttt agtcttataa
    15601 aatccattct atcctccttg tttaaagaat aatgcatcac taacaataat attatactac
    15661 aaaaaataaa ataatagggt attataatac cctattatac ttaattactt taaatttttt
    15721 aatattaatc tactaatacg actttttttt ctaaaataag acacttccca ggcttaattg
    15781 gttcatcaag ttttatatca ttaagttctg caatttcatt ttctgttgta ttgtattttt
    15841 ttgctatatt ccagaaagta tctccttctt tgcatatata tacgattatg ctaggagctt
    15901 tagataaatc atatacacct tggtcttctc ccttgataat aaaattttct gctttcttat
    15961 ctaatgcttc agtaaatctc ttaattttga taatcaagtc tatctggtcc ctattcaaat
    16021 caacttcgac tttatcaata catgctgtat taaacacaga tgctgtatca gttagattat
    16081 ccatttctat atcatgttca aatggaattt cctcactaat tttataaacc aattttaaac
    16141 cttcaactgg gacaaataaa atttcaactt ttatgattcc ttgtataatg cttttatttc
    16201 cttcaatata actattttct atagacatgg ttggacaaac gcttacaata tcttttattt
    16261 gtatatcatc attatcattt cttatgcctt ctctaaccat aaatgtttct tcactacgtc
    16321 ttaaagtctt atttaattga ataggtttgt ggtcaaattt tattatcttt tgaggcgaat
    16381 atgcatcttg taatacttct cttgtaactt catctgtaac ttttacctta caacaagcca
    16441 tacaatctat ttctaaaaga cctgtgttgc tttcactatt ttgcttgaaa atatgattaa
    16501 aatctgacat agacaacaat acttcttcag tcataccatc actaactcca gggacttcta
    16561 caaattgagt aaactcaata cctactctat ccaactctac aagttcaccc tcatatgtac
    16621 aagctaacgg atttatttct aatactccac caataataac tttattgtca gtcactctac
    16681 tttcttttac tttaacacat ggatttaaac ttattataga ttgaatttcc tctgtgttta
    16741 ttgttatagt atctcttata gaactttctg ctttttctat tccaactata tcttgaaaac
    16801 aaatttcctt tctgtgcttt tgtatgcatt ccacttgcgc cacatctttt acaatgtcta
    16861 acctttgctt ttcaaacaaa cttcccctta tattcataag agctcctact ttaatctttc
    16921 tctcattcat tatagtacaa tccatatgct ctacttctga aaataacatg tattccatat
    16981 cttgaataac attgtctttt tctattactt cgtttatatc tacttttccg tctacattag
    17041 aaatagtatt tttatcatct gcaatataga ttacattata attaaaacta cctctgcaaa
    17101 gtatcttccc atctgccatt tcaatttttt taagtgatat gtatccttct gtttttacaa
    17161 tttcatatac atctaatttt ttgtctggca caactgcctc cgcttcaatg aatgtttgaa
    17221 attttccaaa gtctattcta ttgtcaactt taattacatc tttaattaat tccatagtct
    17281 acctccttaa gtattataaa aatatcacac tatatttata tttaaggagc aaaaaaaaaa
    17341 ttacacctat ttttaaatag gtataatttt aacttatttg taatttatct tgatctctaa
    17401 aaacttgcaa ctttacatta gatgttaata aatctgagta actataagat actctaggat
    17461 atccattact ttcatcatct aactttataa caaacacgct tggataaact ttttctagta
    17521 taccctcttt tgtaataatc tgctttcttc ctttattagc tttcaacaat attttctttc
    17581 ctatatgtct ctctaagctt actcttatct tatctagagt ttgaacagta gccacaatat
    17641 caccttcttt aacgtttata tgtttacata atatcacata taaacatttt tgtcaaatgt
    17701 ttaaactaat tattttatat attttttgca aaaatgtcaa tactattttt gaagatatac
    17761 agtataatta tgttaatttt tgctaatgta atattgtttt tggcattgcc catctaaatt
    17821 ttcctcttgt ttcagaacca ccaattccct taacacctgt aatagtctgc tctataacct
    17881 catcaatagg aactactaca tcaattcttg aacaagcaat tttctccact ataaaaacag
    17941 ggtctaatgc atgtatcatt attcttccag gcgaacttgc atagtttgct cctgcactta
    18001 ttattttttc ataatttgat tggcatgctc ctgcgaatat gacaagatta tccaaatttg
    18061 gttgccattt acgtgcttct tttactgttt taacaaaatt tgaagagttt ctatagttac
    18121 tcatatcttc tatatctcct ctttttattg tcattgcatc atgacctgtt attactagta
    18181 tatccggatt atatttttct agtagtgctc ttacttcttt atactgattt tgctctgaaa
    18241 cagctacacc tactgcaggt attccaagtt gagcatatac atccaagcaa atttttaaat
    18301 attctttatc tccatctatc tgtagtactt ttccaggcat tccatatgtg ttaggattta
    18361 attgaagttt tggaactgct ctagtcattc ttttttgtct ttctttagct tttcttactg
    18421 acttatataa taaattttct acatttttat ctattaatat atcttttata tcaggtgctt
    18481 ttactaattc taggtcatca atataagcat ctgctattac tctaaatgca attcccttta
    18541 gtatagctat tttctcatta ttttcatcta caccaaaaga gactatttta aatactatat
    18601 ctttgttatg tgactttcta gctacaatgt ctcctacctt catagttaaa cctccaataa
    18661 atttttatct tagtttatac tatgttcgat ataaaacttt gtttcctaat gtcaaaaaaa
    18721 catttcttat aatacttctt ttatcactct taaagcattt ttatagtata tcttttctat
    18781 atcatcttca ctaaaccctt ctttttttag aggttcatac agtttaccca tctgagatat
    18841 atcttcaatc tcaacttttg aatctattcc atcaaaatct gaccctagag atacaacatc
    18901 tattcctcca acatttacaa tatgttttat gtgtctaacc atatcttcaa gtttactttc
    18961 acttttatca cttaaaaata agtggaagaa gtttatacct gtaactccac ctttatttgc
    19021 taatacttta atcatatcat cagttaaatt tctagaatga ttcatcattg ctcttgaatt
    19081 agaatgtgta gcaattattg gtttagaaga aatctttgca atttcataaa atccaccatc
    19141 tgaaatatga gaaacatcaa ccaacattcc caattcattc atcttatgta ccacttctct
    19201 tccaaaatca gtaagccctt tttctctata ttctacttta ttatgaggaa agctcaattc
    19261 attcacatga ttccaactta ttgtcatcat tctaacccct aagtcgtaaa atttctttaa
    19321 attttctaat ttaccttcaa gtactgcacc ttcctcaata gataaaagag ctgtaagttt
    19381 accttctgat tggtttttca taatttcttc atagttagta gctaaagcta tttcatcact
    19441 attttttttc atttcctcat gaaatttatt tgccatactc atgcaataat caaaaggatg
    19501 ttttacttct tcagtatcaa caaaaagagc aaatgtttga gctaatgaat cccccttttt
    19561 caatctgtct atatcaacag aatatttatt agacttaagt tcacttgttt ctacattttc
    19621 cattaatttt gctatagtat cacaatgtaa atcaagaaat ttcatattta taccccctgt
    19681 atattttata tttatatcta aaaatagaaa tagttgtcct tatagacaac tattgacaac
    19741 tattttttaa tattcttttt aatattactt aatcttattc ttcttttaag ttaggtatta
    19801 cttttttaac ttcttcaatt tgatgccatg ttttatctat attattttca ctaatatatt
    19861 tttttaaatc agttaaagat gtaaaatctt taactgataa attgtcatca tcatctgcaa
    19921 atcttaaaat aaacatgaga ctcccttact ttccacaaca tttcttatat ttttttccgc
    19981 ttccacatgg gcatgaatca tttcttccta tttttgcttc tttaactatt gttttagact
    20041 tcttataagc ctttattatt tcttttcttt ggtctgctgg tagtatatct tcccaacctt
    20101 gtaaattata tagccaatga gcctcaacag ttaccatatt gttatataat ttttcccaat
    20161 ctatttttat agaaacttct ttatcagctt ctacttcttc taaaactata tcttctttta
    20221 agctttcact tattccatct atgaatccca tgaaaaattc attagtagtt ccaaattttt
    20281 cagccaattc acttacttta ccttcaacaa catccatttt attatttaat atttcattgt
    20341 atattccagc ttcaactttc aaatattctt cccagaattt tacttctgct tcttgacttt
    20401 catgattatc acttaaattt ctccattcag tatataaact catacttttt cctcctaagt
    20461 atacctatat ttttatagtt tttatagtat aaaatctttt agttagattg gaagtttctt
    20521 ctttttgatt ataccatatt ctatttaaag ttaactctta tattatatca taattaaaca
    20581 attcttttaa tacttttata gcatactctt ggtttataaa ataagggctt gtcgcaattg
    20641 ttattacttt ggctttttta attaagtttt ttatcttgta aaatttgagt tcaaatggta
    20701 tatagtccat atcacttgaa aatatatcca aatctatatc aagaacaaac tctgattcta
    20761 cttctaaatc aaatccatat agactatcta ttattattaa ttctgaaaat atgttatatt
    20821 ttaaagcagg ttttataaaa cttcccacat ttaaaacttc atttgtatat ctaaatacat
    20881 cttttagatt atttacatct acatcatagt tctcaggttc tctagtatcc ttatgttggt
    20941 ctacatgcac taacttgcat ccttttgtaa actcaccctt taacaaagat tttgcccaaa
    21001 aataaaatgc atggttatga ttgtcaaaca catatatctt tttatttccc aggacatatt
    21061 cgaccatatt ctccaatcct tttgctttta tttccttatt ttcatctact tcattaaata
    21121 ctacctcatt gcctaacctt acatcattta atgtaccatt tattagtttt ggaacgtata
    21181 tgctcttatt ttctctttta tcatatgaaa atacattatt tcctactggt ttttctatat
    21241 aaaatccact atattcataa ttttccataa aactttattt cctccattgt tatataaata
    21301 tataagttat aattttaaaa attaaaatgc acttattgta aatcatatca tctattatag
    21361 aaaacatctt atataactaa atataatatc ttctctcaat atgtattata caataagtgc
    21421 aaaatatatt tatataaaat atactacatt cctataacaa aaccatcagc ttcaattgga
    21481 aggtaactaa ctcttctatt caactccacc tttccaaaaa atactaccca atatggtcta
    21541 aaaatacttt caattttaac tatttcaatc tctgggattc ctcccataaa cctatgagtt
    21601 aatctcatag ccattttatt tgcctttgct tttactcttc gttcgtcctt atcactatac
    21661 tgcacaatat tacaatccac attttttaca acttcatatg taggaaaatc ttgtactata
    21721 ctaccagaac ctgtagaacc attcactaaa atttttaatt catttatatc attagaatat
    21781 ctcctattct tgaaaatgga tttcgttttg tgaattttaa gtgtgataat tttaaactct
    21841 atataatgaa gtctaacttc atgagcatat ttgttcttta atattagctt actaaataaa
    21901 gaattttttc cacttttagc tatctctata gcttcttctt ttgtaattct tataggtaac
    21961 gctcttatat ccataaaatc attctctttc ttattttttt cttgtcattt ttaaaactct
    22021 atatatataa tcaaaaaata ttacaattac aactgccata actccatatg gaattatttt
    22081 tccaactata atatcatttc tagtcaatat accataaaat ataaatcctg ttattaaaaa
    22141 gtacaataat actagaaaac cgtctattcc aagccatttt atttctaaat ctttttttac
    22201 cttagacact aatttagaca tagcagtttc tccttttata gattattttg tgtatttaaa
    22261 ttttcaccga ttctattacc aatcatccat gcaataaatg aaaaaggtat agctatcaca
    22321 agaggctcta actcaaagct ctgaactgga aatattttaa aataaccaaa agtcactcca
    22381 ccaacaatca ttcctaaaat accacccata gatgttcttc ttccatttcc aagaagccca
    22441 cctactatag gtacaaatgc cgaacccatc caaatacttc ctacaaatat acatgcatca
    22501 tatacatttt caaatctaaa tgctacgcta agccccataa ctgctgctaa acatacacct
    22561 attctagtaa actttactaa ttgtttctga gataactctt tttttgtaat tcttccatat
    22621 atatcatttg ctaatgtagc cccagctgct agataatagc tatccaatgt tgatattata
    22681 gaacctaaaa ttcctattat aaatattact cttattcctt gtggtaatgt atctaaaact
    22741 aaagttatat atccagcagc tggagccatt tctggataag tagctcttac tataagtcct
    22801 gtcatagtta acaccatgtc aaaacatatc catagtgaaa aacaggttaa taaggctctt
    22861 cttccaacct taggagaatc tgctgctgaa aatctttgat aaaaagttgg gtctgaataa
    22921 gctccaaaag aatatacaat taacatcaat gctttcatag ctgacatacc ttttgtaggg
    22981 tgcataagct gtggtgcatc tttgagtgct tctgtaagac cttgaaaccc tcctatttgt
    23041 tcaaattgac ctggcataac catagttaaa ccaaatacca tacaaaaaaa caataccata
    23101 tctgtaatgg caactcccat aagcccacca agtactgtaa gtacaacgac tactcctacc
    23161 attattaatc ctattaattc tttatctatt ttccatgcta cgttacctat agttcccata
    23221 gcagttattt ctccaagttg tgcacaatat ataaacatca aaattgcccc aattatagca
    23281 acatgcttgc cataaaaact ttccagtaaa tcaggaactg ttatattggt tcttatatga
    23341 atccttggac caacccacaa tgctaaaggc attctcccaa tatgtgcagc tattgaccaa
    23401 actgcccatg cagatattcc atatatagcc gcatactcta cactacctac tgtaccaact
    23461 cctccatacc atgatgcaac caaagttcct actattaagg accatggtaa acttcgattt
    23521 cctaggtaat aactttccat atcttttaca tttctagaaa aataataacc tattcctaat
    23581 aatataaatg caaatataac tataatccca ttttcccaaa atgttaacat atctaatctc
    23641 cacattctta tattttaatt tttaacttta atatctaaat atgaacaact ctacccattt
    23701 ccataaaaat aggtagagtt atataataat tataagtatt taagtaatct tgttattaag
    23761 caattttatt gtttaaacct ttattttctc ttttatttat tttatttaaa gcataagtag
    23821 caaatatccc tataactgca catactacag ataaaataaa tatatatgta tatcctgttt
    23881 gtccatattt atctaaccaa ttacctgcaa tagtgtaata aaacgcatct ggagaaaatc
    23941 ctataaatga agcaaatcca actactgctc ctgtagtttc taaagatata ttagactctg
    24001 aaacagatgc aaagtatata cctctaaatc caaataaaat tacacttagt attatcatgt
    24061 ttattacagc tatccatata aatcctgtac tcttaggaat aaccaagtaa gcagctgtac
    24121 ttactgtcat taatataaat cctacaaaca ttactcttat agatgaacca actttatctg
    24181 ttataacacc agcaactgga gatgctccca tttttataac ataagttctt attattgata
    24241 aagcagaaac tagagtcatt ggcattacat aaacttctga taagtatgga cttaaataag
    24301 ttaaacttga gaatactaaa taagttgaaa atattataag accaattaac caagcctttg
    24361 gtattgtaac tacttttaac aatgacttaa tacttaatcc ttcatctgat gctccactat
    24421 cttttttctc ttccactaag aaaaatagaa gtataccaca tattatagat gctgcagaat
    24481 ataaccatac tacatatttg aatcctactg ttatttcagc aaatttacca aagaaataaa
    24541 gacctataaa agaaattaat gtacctgata cacctgataa cccttcataa aaaccaaata
    24601 ttcttccttg ctctgactca cttccttgca ttctaacaac cttaacagag gctgagaaga
    24661 aggttaatat tgtcgtaaat ccccataata caaatattaa aagtatcata ctatatgatg
    24721 ggaatgtaga gaaataaaat ccaagtatcc ctgaagcaat taaagaaaaa gttattaatt
    24781 ttctaactga gaatctatct gcaaaccatc ctcctataaa ataagatacc attcctacta
    24841 atccatataa actcaataag ttccctattt gtgtatgact taatgctaaa gcttgttgca
    24901 ttgggtcata aaatgatgat ttcatatatg gtaaactata catagctgtt gttccaaatg
    24961 ccaatatgaa tattatcaaa aatcttttaa cattattttt gctttccatt gattctctct
    25021 ccttaaaatt ttattgttta taatctcatt catataattt tattttaatt tttctattgc
    25081 ttgtttaaaa tcatttatta tatcatttga agattctaat cctacagcta ttcttattag
    25141 attttcccca ttcatttgaa ctaatgtatg ctcttcccct aagcttgttg ctattgttgc
    25201 aagctctaaa gagttcatta atcttctact agcttggtct cttgtcaatc cactgatacc
    25261 atccttaagt ttaaatgaaa ctacgcctcc acctaaacca ttcatttgag ttttacaaag
    25321 ctcatattga ggatgtgatt ttaatgctgg atactttacc aattccacat tctcttcttc
    25381 ctctaaaaac tctgctactg ctaatccatt ttcacaatgc tttttcatcc tcatatctaa
    25441 cgttttcata ccccttaaaa tcatccatgc actcattggt ggtaaagatg ctcctgtaaa
    25501 acaacataaa cttggccatc taactaaatc tattaattct tttgaacctg ctactgctcc
    25561 ccctaaagta tctccatgtc catttataaa cttagtaaga ctatgtatta ctaaatctgc
    25621 acctaaatct attggtcttt gtacaattgg tgtcccaaaa gtactatcaa ctatagctaa
    25681 gcttttattt tcatgagcaa tttctgctat tgtttttata tctactaagt ctaatgatgg
    25741 atttaaagga ctttctatat aaactatttt agtatttggt ctcattgctt cttttacatt
    25801 ttgtggattt actgcatcta caaatgtaac ttcaactcca aatttagata acaactcaga
    25861 catgaataat ttagtgtgtg aaaatattcc atctgaacta actatatggt caccactttt
    25921 taccaatgct aatatagtag atgttattgc ccccatgcct gatgtactag atatggcact
    25981 ttctgcattt tcaagcatag ccaacttagc ttctaactct gtaagagtag gattacactc
    26041 tcttgtatac acatatcccc atgtcataca caactcttca aaatgctcta ctgtctttgc
    26101 agcaaatgtt gctgtttgac atattggtgt agccaatgca cctgttgttg ggtctgcact
    26161 atgtccccaa tgtattattt ttgtctctaa atcttgatta atattgttac tcattttgat
    26221 ttcctcccat tttgacttat ttatacaacg ttttccctgc caaatttcat caatttagct
    26281 tagtcttaat acattttttc aagtatactt ttttatttgt tataatttct aaaaatatga
    26341 cacttatttt gtttttttac agttctaatt ataaactcta tagtaactat agagtcaata
    26401 tgtttttgat atttttttta caatgtttaa aatagtcaat acaaaaatag gtatatctta
    26461 gaataactaa agtcatccta agatatacct atttttatat cacaatataa ataattacat
    26521 ataagcatta gtttttttat cataaataga tttttcaaga tttatcattt tttcataaat
    26581 atattatagt taaatatttt aatattacct aacatctaca ataaaatata ctcttaaaat
    26641 aaaattatat aattaaaaat actctataaa agctaaaaag tttttctatt cgctacatct
    26701 aaaaataatt agatagaggt ataaatgtat ttagtttatt tatacctcta gtcttttata
    26761 aattatatct ctactaaatt gattttttag cacgaataag tgtgctcttg gagataccag
    26821 tcatcttcac aacctctcta tatggcttag aatttaataa ccccaatgca tattgtatct
    26881 gttgattgct gtatttcttt ggcctaccat ctttaaagtc ctttttcttt ttagcaaatt
    26941 cttttcctaa ttgagtacgt tctattatca tatttctctc aagttctgca actgcaagta
    27001 atgtagtcaa aaagaattta cccattgaag tattttctaa aaggcctata tttaaaatat
    27061 gaacacttac ttctttagca aataaacctt ctattagttg tattccttct gatgtacttc
    27121 tagctagtct atccagctta gttgcaacaa gtaagtctcc cttttttaga gatgatataa
    27181 ttttttcaaa tttaggcctg tttgtagtag tgcctgtata agtttccttt attattttag
    27241 catcattata tttattcaaa atttcttgct gttgctgata caaactgttg ttgtctgttt
    27301 gattttttgt acttacacgg caatatccat atataataat catcacttcc taacataaag
    27361 attttgtaca gaagttaccc agaagcttgt ttttacttat ctttatccat tttactaaaa
    27421 cgatttttgg gtgtcaatac tgttaacttt tgacaccata ataattcctt tataatttgt
    27481 ttgactatgt caaaaattac caatataatg aaattaatga gtttatacaa attggaggaa
    27541 ataatatgaa tagtctgtta aaagaagcaa tagatgaatt aaaaaacatc cattgcgggg
    27601 aagttttttt attaaaagaa ttgttcaaag gctatgaatg gaacaggttg gagatgaata
    27661 tcagattgaa attaggaaaa ctttttttgt atgaagtggt aactaatccc aatttaaatg
    27721 tagaggtcat caataaaaat tctgcttatc agcaaatgta taaaaaaaaa tagtgactta
    27781 aatgtatata atagtataat cttcttattg attcaaaagt taaaactttt aatacaaaac
    27841 aatcattcta ttttaacatt tatcaatatg tctaaattta tataattaac acatacgaat
    27901 agtattgttt aaatcacatt atttaagata gataaatctt aaccctactc cagcataaat
    27961 actaaaatag ggcttagaat tatctatatt tttatcccat aaaatgctta gctcttcctc
    28021 ttgattgaac atatatatct ggggaaatac aatagaagcg taaatttata aaatttccaa
    28081 agtcagaaaa cttcagttca aacttaggta taccaaaact ttcaggtgct atttcgtcaa
    28141 cgccaaaata gatgataatt ccattatctg taagataaaa tgcttggtct tcaggtataa
    28201 ctaactcaac attagaataa tacatttctc tattttcatt tatttgagat ttaatgtaat
    28261 cagtaattat attaatataa tcaactccat ctctaaaaat gtcattaagt aaaagttctt
    28321 ttccagttgt caaatctata ttgtaattat tcaatgtatt gtatttaggc ccatagttat
    28381 tagcaaaccc cataaggttt aatatcatac tcagtacata atttttatta aaagtaactg
    28441 cgtaactggt agatacccaa tagctttgtt ttggaagtga attttgagaa gctgcgttat
    28501 tatattctcg ttcttcttcc tgtaagccat ttttaaaatt atttacagtc attcttacag
    28561 cattgtttat gttatttaaa ataactgggt ttacatacgt ggattgattt ctaatattgt
    28621 atataacagg atactttaca tcatatgaaa aatacttact tctttcaaaa tcttcattaa
    28681 attgtacata taatatatta tatgtacagg tatttgtaga ttgataattt tccattaaaa
    28741 tcctccccct tttataaaaa aatcagatac aatataatat gcttaaaaca gtaataatgt
    28801 taatggtaaa tgtaaagaat agaaagtatc taaaacagca aaaaatgact aattttgtgg
    28861 tataatacat aggattgaga gttattttat aggaggaaaa atatgttatt attagcagat
    28921 aaatggaaag attatgaact tatagatatg ggaaatggag aaaaattaga acgttgggga
    28981 agttatgtac ttagaagacc agacccacag gttgtatggc caatggaatc tgaatgggct
    29041 ttatggaaaa accctcatgg acattatcac agaagcaata aaggtggagg tcaatgggaa
    29101 cacaaaaaaa gatatccaga acaatggact ataaattata aaaacttaaa gtttcacatt
    29161 aaaccaactg gctttaaaca tactggatta ttcccagagc aagcagctaa ctgggattgg
    29221 atgatagaga aaataaagaa agctaacaga cctataaaag tgttgaatct ttttgcatat
    29281 acaggtggag ctactgtagc atgtgcatct gctggtgcag aagtatgtca tgttgatgct
    29341 gcaaaaggta tggttaattg ggcaaaagaa aatatagaat tatcaggtct taaaaatcaa
    29401 acagtaagat ttatagtgga tgatgttgta aagtttgttg aaagagaaat aagaagaggt
    29461 aaaaaatatg atgcaataat aatggaccct ccttcttatg gaagaggtcc taaaggagaa
    29521 gtatggcaaa tagaagaaaa gttatacagt tttgtagatt tatgtatggg agttttatca
    29581 gataaccctt tatttttctt aattaattct tatacaacag gattttctcc aatagttctt
    29641 gaaaatgtat tgtctacaac aataggtaaa aagacaaata caggagatat atacggtggg
    29701 gaattaggaa taccagcttc cagagatggt aagatacttc cttgtggtat atttggaaga
    29761 tgggagaaaa aataatgata aaagtcatat atgaagataa ccatttatta gttgtagaaa
    29821 aaccagttaa catattgtct caaggagata atactaatga taaagatatg gtaaatcttt
    29881 taaaagacta tattaaaaaa aagtataaca aacctggaaa tgtatatatt ggtttaatac
    29941 atagattgga tagaccagta ggtggagtta tggtgtttgc aaaaacatca aaagcagcat
    30001 ctagattgtc tgagcaggta agaagtaaga ctttcaaaaa aacttatcta gctgttgttc
    30061 atggcaagat aaaatctaac tcagatatac taaaagattt tttatataaa aataaaaaaa
    30121 ctaatatggt aactgtagtg aaaaaaagcc ataaagatgc caaagaagct gaacttaact
    30181 ataatgtatt agattttaaa gaaaatctaa gtttagttca agttaattta aagactggtc
    30241 gttctcatca aataagagtt caatttgcaa gcagaaaaca tccattatac ggagaccaaa
    30301 gatatggcaa agaaataaat aagattggac aacaaatatc tctttggtcc aataagatag
    30361 aaatttgcca tcctactaca aaagaaaaaa tggaatttac atgtcatcca cctgacaaat
    30421 atccatggaa tatattttaa atctcaaatg aggtgattat atgagtaaaa aattttcaat
    30481 aggagaaatg tctaaacttc ataaaataag catacagaca ctaaggtact atgaccaaat
    30541 agatttattt aaacctgttc atgttgacca agaaagcaat tacagatatt actcaattga
    30601 ccaattttca caattagata ttataaaata tttaaaatat ttaggtatgc ctttaaaaga
    30661 aattaagtca aagcttgatg aaaataatga agaaattcta aaattactaa caaataaaat
    30721 taatatagtg gatgaaaaaa ttaaagaatt agagcttata aagaaggtac taagcacaaa
    30781 gaaagaaact atggaaaaag gtatgaaagt agatgaagtt ggtaaaatag tgagaaaaca
    30841 tattccacga agaagtatct tatctataga gtatgaagaa ggattaagta gtgaagaaac
    30901 tatggaactt tccagaagac aaatagcaaa tatattagaa gataatatat ctgtgttcta
    30961 tggtggagta agtggacttg tatctataga taaattttta aaagaagata aagttattta
    31021 tgacaatagt tttgttactg ttgaaagaga cttatttgat ataaaatcac aaaaacatat
    31081 aacagaaatt tcatctggtg attttatttg tatgacatat aaagggcctt acaaagaaaa
    31141 ttataccgct tttaagaaac tggttgatta tgttgaatat aaaagaatac ctattgaaaa
    31201 ttatatatat gaaataccta taatagaccc tctgtctaca aacaacgaag aggaacttct
    31261 aacagaaatt caaatacaag ttaaaaagag agaaattgac ttaagatgtc tagtgtagta
    31321 atctagttta cttacacatc ttaatatagt tgtctattat caacatatga tttttctaga
    31381 tagtgattcc taacagtgag attttcaaaa aataggagga aatatggcta atgctaattc
    31441 cctcctattt ttaattaagc catatcattt taattaagcc atgtcatcac gaagtgcatc
    31501 aataatattt tcttttttta tcttattaat agtatacaac attgtaataa atactataaa
    31561 taaaacacta aatatactaa ttccaatact aaaccatgga atcacaaaat caatatcatc
    31621 tgtaaccatt actttatgaa ttagccatga agaaattact gctataggaa gtccaaagaa
    31681 caatgctctc atgccataaa aggcacactc aaaattcatc atcttttgga aatcatgctc
    31741 agacattcct acagagcgaa gcatggcaag ttctctacga cgtaacttaa tattagttga
    31801 aattgtatta aacacattgg caatcgaaat cagagaaatc ataattatga aagtataagc
    31861 aaaaacattg gcaataaaaa tgtaattacg gttctcatcc atcattccat acatattgta
    31921 cagattgtac tcattcgtaa ctccctcact ctcaataatt tgctccattt ctgcaactga
    31981 ctgagtagga tttttcgatt gaaagcttaa actttcaatt cctgtagaaa caccaggaat
    32041 ctcaaactta tctttggctg aataaggagc caatactctg aagcttgctt tcttcacatc
    32101 tgaacttctg attcctggag gagaatcagg tggcactact ttgacaaatg aaatattcac
    32161 attttgtctc tcaggtatct ccaacttgcc attagtttca ggagtgaggc taacattcat
    32221 agaagaactg ttgaaaatat caataatatt attatccttt gttaccatag aaacagcaat
    32281 cagttttgca ttttgtccag tatattcttc tgctggtaat ccaagacttt taataatatt
    32341 cagataagtt ctatcatcaa gaaattgcat atctactggt aattcaagca tttcactcat
    32401 agaatgtgaa ccctcatagt tcaaataatc gtctgtaagc tcacttactt tggcaacaca
    32461 tgaataggtc ataagtttct gatacaaacc ttcgtaaaca ccatttgcag tttttagctt
    32521 atcgtaaagc ttcaacattt cgttgtcttt cataccttgt gtagaaaaaa taatatcctg
    32581 gtcagtaacc tctgacctct gatttgacat ctgttttagg tctgctacta aagcacttgt
    32641 agatataaat agaactatgc ttaaaacaag cgacaataca atacttcgat aacgtttctt
    32701 gtttctttta aagtttttca tagcaagaac tccctcaaga ccataaactc gctgtgttag
    32761 ttttgatgtc ttcacagatt tagattcaat ttttacctca tttgtctgac gaatacactc
    32821 catcactggt gtgttggcag cctttcttgc tggtatgtaa gcagaaatta agattgtaac
    32881 catacttacc actgctgcaa cgatgattgc agggatagac aatgttaatg tcaacggaac
    32941 gtttacatag agaatatttg caaagttttt agatatgaca gaaatcacaa gtccaatact
    33001 gactataccg attgcaacac caattggtat accaatcgca ccaatgcaaa ttccttcaaa
    33061 tagcaccgaa tttcgtaact gttttgctgt agctccaaca gatgaaagta ttccaaattg
    33121 atgtgttcgc tcgttcaatg acatgttaaa tgaattatat attagaaaaa ttgagcctag
    33181 catgattatg gcaagcacaa tgccaccaac cgagtacaaa aacatattga acaaattgtt
    33241 atcagagaca cctatgaatc gcaacacatt gtcattgaaa atggaagcat ggtctttagt
    33301 gctacttgtg taagagcgaa tctgatgtgg atttttcaat gtgacaaaaa ggctaagact
    33361 gtcagtcttg acttgtttat cttctttggt tatcaaggta tatcctggtg cagaagattc
    33421 ttcaaatacg ggctttctac agatacccac aactgtataa gttttcttat cctgtggcac
    33481 aaaaacttca ctcccagcag tgtatgggtc actctgacca agcttttcat tttcactcat
    33541 tcgtttccct acatccaaag aaatagtatc ccctactgca aatttgacac caccatctgt
    33601 cgcaacttta cttgaaacaa gaatctctct actattttca ggtaatcttc cagaaattaa
    33661 agttataggc aaagtatcaa aagcttcttt gctaaatcca gcaataaaaa gatatggttt
    33721 atctggattt ctgttacctt caagtgttgc atagccaata ttttcaaatg ttgcagtatt
    33781 tgcaacttca tcattgactc tttgttcctg tacaaaagaa gaatccacat ccaaaaactg
    33841 gacatgccaa tcaccatatt ttataatagc tccatttatc atatagttca tcaaagaaac
    33901 tccaaaggta gtaactgctg taatcatagc agctgacaga acaactccta taattgttac
    33961 aagagttcga gttcgattct ttttcaatcc ttgcagagca actttgttaa aaatgttcat
    34021 taacgattca ccttcctctc atcacgcact acttttccat ctgaaataca gataattcta
    34081 tctgcctgta aagcaatgtt ttcatcatgt gtgacaataa tgagagtttg gtgatattta
    34141 ctatggctct ctttcagtaa ctggataatc tcttgtccat ttttactgtc taagctacct
    34201 gtaggctcat cagcaagcat aactgctggt gcattcatca atgcacgacc aatggcaaca
    34261 cgttgctgtt ggccaccaga aagttgattt ggtaaatgtg tttttcgttc ttttaaacca
    34321 agtaattcta acaagtcatt taaccgttct tgattgacct tacgcttatc cataagtata
    34381 ggcaatgtga tattttccac tacatttaat gtgggtatca gattgtgaaa ctgataaatc
    34441 aatccaactt gtcgcctacg aaaaatagca agtttttcat tgctttggcc atatacatct
    34501 tgcccctcca gatatatctt tccacttgtt ggtacgtcta ctcctgcaat ggaatgtagt
    34561 agtgtagatt taccagaacc agaagaacca atgattgcag taaactcccc tttttcaata
    34621 gtaagtgaga catggtcaag tgcagtaacc ttattttcat tcttaccata aaccttacat
    34681 aaatcttcaa tctttaaaaa cgccattata taagtctcct ttcatatttt ataaatatat
    34741 catagaactt tcaacttaca tttaagtgac atttaaatga gagattcgtc actttggaaa
    34801 acgaataaca aatattgcac caccttgtgg gtgattttgt gcagtaattg ttcctccttg
    34861 tctcataata atcgtcttac aaagtgccaa tcctattcca taaccggctg tgcttgaatt
    34921 tttcccacga taaaatctgt taaatagaca tggtaaatct tcttttttaa agcctgctcc
    34981 gttgtcatga atagtaagtt gagtaaacaa aaagttgtcc tcacaaatga tatcaatttt
    35041 tccattatca cccacactct ccatgcagtt tttaaatata ttttgaattg cttctgaaag
    35101 ccaatttaaa tcacccaaaa tccttactcc ttttggtata tcaatatgta aagtgatatt
    35161 gtgcagttcc atgggaatca aaagttgatg aagtgcagaa ctaatcaaat tattcacatc
    35221 aatttgttct ttttgaaaga ctacaatacc tgcatctaaa cgtgataatt ttaaaaggga
    35281 agtaatcagc cagtccattc gtataagtaa ttcctctgtt tctcgtatca actcttttct
    35341 ctcatcttca tcagaagtat tctctaacag tgacaaaata agagttactg atgtaagagg
    35401 agtgcgaagc tggtgggcta tgtcagctag tgagtcagca aggtgttctt tttctttttt
    35461 cagtgcgtaa ttttgctctc taatgcgtaa cgtcatttta gttatttcac tttgtagaat
    35521 agaaagctca ccctcatctg attcagcaat atacagatgg ttagcattat gaagcacaag
    35581 attaatttgt tctgaaatct gtgcaatact tttgtatcga tttttagtaa acacaaaaaa
    35641 tgctattcca aaggtagtgg cagaaacaat agaaagtatt ccagtcataa tactgattgc
    35701 aaatcccagg gctacagtga gaatagtaat taataagaat aaaatagcaa attttcgaaa
    35761 ctctctattt cgaaacataa cttcccccca atctataccc tgtcccacga acagtcaaaa
    35821 ttatttgtgg acttgaaggg tcatcttcta tcttctctcg caagcgtttg atgtacacag
    35881 tcaatgtgtt gtcattaaca aactcacctg ccgcatccca caattcatca agtaatctta
    35941 ccctcgtaat aatactttta ggattgctaa taaacaccaa taacaaacgg tattctaaag
    36001 ctgaaagaaa aacctcattg ccattctttt tcacaacacc agttgctgta tctacataaa
    36061 gcccacaaat ttcaaaagtt gacccaaaac gcccactttt tcgtagtgct gttctaattc
    36121 gcgcaatcag ttcacgtgga cgaaaaggtt tagtaatata atcatctgca cccatattca
    36181 gtccagtaac aacacttgat tcatcaccgg aagctgtcaa aaagataaca ggaatgtctt
    36241 gcttttcttt gatttctgtg caaatcgtaa acccatttcc atcaggtaaa gaaatatcaa
    36301 taagtgccaa atcaaattta ttcttagcaa tctctacaag tgcttcactt cttgtggata
    36361 catgggttac tataaatcct tctgaattaa gcaaaagaat aagatttttg gcaattgctt
    36421 tatcatcctc aactaaaaat atccgtttca tttactttca tcctccttta ttttattagt
    36481 tttatatatt ccagcagagc aatagtacac tactatttta cttatcatta cacaattttc
    36541 agtagtttct tttgttctta tataatccat gcataatacc tccattgatt tattatattt
    36601 cttataacat aaatacacaa gataagacaa aaaacataat gtaagtgcaa gttttttaac
    36661 tcactaactt ataaacatac actcctacta acactacttt cttgatgaca catcttacaa
    36721 gcttatattc atacatcaat gaaaaattaa atttatccat ttattaaaat aaaaggcaat
    36781 agcttctata gctaatgccc tttatcttaa aatgatttaa atactataca atttttataa
    36841 atggctataa ataaactcgt atagaatata aaatttatct acagtctttt gttatacaaa
    36901 atttataacc tcttatagaa caaatttacc actttgcaaa cttatcactt ttactcagct
    36961 atattataaa cttttaattt atctttgttt aaatgtttct tcttaacttc tactaaagct
    37021 tttagcgtat ccaaagatgt cattatttca gtagaaaatt caatggctgt tcttctcatc
    37081 ttaaatccat ctcttgtaga gtcatttcct ttagttggtg tatttattac tatatcaact
    37141 tgtttatttc ttatagcatc taatatgttt ggtctaggtt cttcaattct atttactata
    37201 tcagcctcta ttccattttc tcttaaactc ttcgctgttc cttctgtagc aacaaaagta
    37261 tatcctaatt ctttcatatc ttttgctatc tctataaact catctttatc atgattattt
    37321 acagtagcaa gtactactcc tctttcatca gacatatgtc tacctgctgc caagaaccct
    37381 ttatataagg cttcttctaa attttctcct acacctagga cttctcctgt agacttcatt
    37441 tcaggtccta gacttacttc aactttagat aatttagaca ttgagaatac tggtactttt
    37501 actgatacta attttggctc tttgtaaact cctgtaccat atcctaaatc ctttaattta
    37561 gctcctaaca tacacttagt agccaagtca actattggta ctccacttac tttacttata
    37621 ta
//
LOCUS       DAYASV010000024        35263 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYASV010000024 DAYASV010000000
VERSION     DAYASV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35263
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.024"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            106..756
                     /locus_tag="ACGI68_001251"
     CDS             106..756
                     /locus_tag="ACGI68_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0137696.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(913..1932)
                     /locus_tag="ACGI68_001252"
     CDS             complement(913..1932)
                     /locus_tag="ACGI68_001252"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFH0137697.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(1963..2577)
                     /gene="plsY"
                     /locus_tag="ACGI68_001253"
     CDS             complement(1963..2577)
                     /gene="plsY"
                     /locus_tag="ACGI68_001253"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFH0137698.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(2612..3937)
                     /gene="der"
                     /locus_tag="ACGI68_001254"
     CDS             complement(2612..3937)
                     /gene="der"
                     /locus_tag="ACGI68_001254"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFH0137699.1"
                     /translation="MSISRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRI
                     FAEVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAA
                     DKEIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLD
                     EIVENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAID
                     TYFEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTE
                     QDTKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAK
                     TNQRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTD
                     IRPPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(3965..5293)
                     /locus_tag="ACGI68_001255"
     CDS             complement(3965..5293)
                     /locus_tag="ACGI68_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HFH0137700.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(5531..5731)
                     /locus_tag="ACGI68_001256"
     CDS             complement(5531..5731)
                     /locus_tag="ACGI68_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137701.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(5733..6665)
                     /locus_tag="ACGI68_001257"
     CDS             complement(5733..6665)
                     /locus_tag="ACGI68_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFH0137702.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(6710..7642)
                     /locus_tag="ACGI68_001258"
     CDS             complement(6710..7642)
                     /locus_tag="ACGI68_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HFH0137703.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(7697..8986)
                     /locus_tag="ACGI68_001259"
     CDS             complement(7697..8986)
                     /locus_tag="ACGI68_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137704.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(9015..9707)
                     /locus_tag="ACGI68_001260"
     CDS             complement(9015..9707)
                     /locus_tag="ACGI68_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137705.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(9722..10483)
                     /gene="pgeF"
                     /locus_tag="ACGI68_001261"
     CDS             complement(9722..10483)
                     /gene="pgeF"
                     /locus_tag="ACGI68_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFH0137706.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(10480..10941)
                     /gene="nrdR"
                     /locus_tag="ACGI68_001262"
     CDS             complement(10480..10941)
                     /gene="nrdR"
                     /locus_tag="ACGI68_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFH0137707.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(10963..11226)
                     /locus_tag="ACGI68_001263"
     CDS             complement(10963..11226)
                     /locus_tag="ACGI68_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HFH0137708.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(11330..12103)
                     /gene="sigG"
                     /locus_tag="ACGI68_001264"
     CDS             complement(11330..12103)
                     /gene="sigG"
                     /locus_tag="ACGI68_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HFH0137709.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(12195..12917)
                     /gene="sigE"
                     /locus_tag="ACGI68_001265"
     CDS             complement(12195..12917)
                     /gene="sigE"
                     /locus_tag="ACGI68_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HFH0137710.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(12942..13787)
                     /locus_tag="ACGI68_001266"
     CDS             complement(12942..13787)
                     /locus_tag="ACGI68_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HFH0137711.1"
                     /translation="MVYIEYYVIENLVINYIIISCTSILTKRYNPKKNKLLGASLGGA
                     YSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLVNIFISGSTY
                     FIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTININLLEKSFCC
                     KALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQEIIGSLDDK
                     TSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDREYGAIMNPN
                     ILPGF"
     gene            complement(14104..15378)
                     /locus_tag="ACGI68_001267"
     CDS             complement(14104..15378)
                     /locus_tag="ACGI68_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0137712.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(15662..16819)
                     /gene="ftsZ"
                     /locus_tag="ACGI68_001268"
     CDS             complement(15662..16819)
                     /gene="ftsZ"
                     /locus_tag="ACGI68_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFH0137713.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(17009..17362)
                     /locus_tag="ACGI68_001269"
     CDS             complement(17009..17362)
                     /locus_tag="ACGI68_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HFH0137714.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(17461..18135)
                     /locus_tag="ACGI68_001270"
     CDS             complement(17461..18135)
                     /locus_tag="ACGI68_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0137715.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VDLFAK"
     gene            complement(18140..18838)
                     /locus_tag="ACGI68_001271"
     CDS             complement(18140..18838)
                     /locus_tag="ACGI68_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HFH0137716.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(18861..19601)
                     /locus_tag="ACGI68_001272"
     CDS             complement(18861..19601)
                     /locus_tag="ACGI68_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HFH0137717.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(19871..21097)
                     /gene="murG"
                     /locus_tag="ACGI68_001273"
     CDS             complement(19871..21097)
                     /gene="murG"
                     /locus_tag="ACGI68_001273"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFH0137718.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(21115..22245)
                     /gene="spoVE"
                     /locus_tag="ACGI68_001274"
     CDS             complement(21115..22245)
                     /gene="spoVE"
                     /locus_tag="ACGI68_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HFH0137719.1"
                     /translation="MPKENLKKQIDIRVKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(22297..23652)
                     /gene="murD"
                     /locus_tag="ACGI68_001275"
     CDS             complement(22297..23652)
                     /gene="murD"
                     /locus_tag="ACGI68_001275"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFH0137720.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSDNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(23671..24639)
                     /gene="mraY"
                     /locus_tag="ACGI68_001276"
     CDS             complement(23671..24639)
                     /gene="mraY"
                     /locus_tag="ACGI68_001276"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFH0137721.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(24680..26053)
                     /gene="murF"
                     /locus_tag="ACGI68_001277"
     CDS             complement(24680..26053)
                     /gene="murF"
                     /locus_tag="ACGI68_001277"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFH0137722.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVIDGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(26220..28199)
                     /locus_tag="ACGI68_001278"
     CDS             complement(26220..28199)
                     /locus_tag="ACGI68_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HFH0137723.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(28211..28558)
                     /locus_tag="ACGI68_001279"
     CDS             complement(28211..28558)
                     /locus_tag="ACGI68_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137724.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(29039..29974)
                     /gene="rsmH"
                     /locus_tag="ACGI68_001280"
     CDS             complement(29039..29974)
                     /gene="rsmH"
                     /locus_tag="ACGI68_001280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFH0137725.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(30004..30750)
                     /gene="lgt"
                     /locus_tag="ACGI68_001281"
     CDS             complement(30004..30750)
                     /gene="lgt"
                     /locus_tag="ACGI68_001281"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFH0137726.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(31144..32430)
                     /locus_tag="ACGI68_001282"
     CDS             complement(31144..32430)
                     /locus_tag="ACGI68_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0137727.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDRLSKSIERFYPEEPESVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(32445..33710)
                     /locus_tag="ACGI68_001283"
     CDS             complement(32445..33710)
                     /locus_tag="ACGI68_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0137728.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            34026..35123
                     /gene="ychF"
                     /locus_tag="ACGI68_001284"
     CDS             34026..35123
                     /gene="ychF"
                     /locus_tag="ACGI68_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFH0137729.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        10965 a         6486 c         3673 g        14139 t
ORIGIN      
        1 taacgagtat acattgtatt ttgtaatatt attcttgatt tatggtaagt ttaatgataa
       61 aatcacgatt atataaacta tattgattat ttgtaggtga ataaaatgac aaataaagaa
      121 ttacaaaaga aaaggatatt gatgtatttc atagaagctg ctcaaaatat tatggaaaac
      181 gaaggtatag aaaatattac acttcgtaag gttgcagata tggctggcta taatagttca
      241 actctatata aatactttaa aaatttagac catttaatat catttgcatc tataaaatat
      301 tttaaagatt ataacttgaa tatttcaaga tgtatagaaa atgtcaatga tgaatataag
      361 aaatatataa ttatgtggaa attattttgt aagcattctt ttgataatgc tcaagctttt
      421 taccaggtat tttttaactt gtctagtgat gaacttagct atataacaaa acaatattat
      481 gatatgtttc cagaagattt aggtattcat gatagtgaca tatctttaat gattacagga
      541 gaaagtataa aggaaagaaa taagatacta ttaaacaatc ttgtagatac tggatatata
      601 ccagctaaag atttagacat aatcaatgat ataattattt ctttttatca aaatctatta
      661 ttcttaaaaa agaacacagg aaaaaattct gatgatgatg aattaactat gaaaatgatt
      721 tcttctattg aatacataat aaaacatgct aaataaaatt aaaatatggc tgtctcaaaa
      781 tagagataag ccttttttat tgttgaaata ataaaaatat atttaatttc atataaaaaa
      841 gagtgcctcg taaactaaaa agttcatttt gagacacccc taaaataaat ttattattta
      901 ttatacaata gtttatatat catcacctaa aactacttct tccatttcat gctttttact
      961 tctcatcatt agttctattc caacttcttt aggatttgaa cctttcttta ttacagaata
     1021 tatagcatta gttataggca tatctatatc taatttttca gcaacatcat gtgctacttc
     1081 agtagcagtt atgccttcta caaccatctt tacttctttt aaagtatctt ctaaactcat
     1141 ccctttacct atgagtatac cagctcttct atttctacta tgcatacttg tacaagtaac
     1201 tattaaatct ccaatacctg aaagacctgc aaaggtagac atattagctc ccatagcaat
     1261 tccaagtctg ctcatttcac taatacctct tgtcataagg gcagcttttg cattatcacc
     1321 ataccctaat ccgtcacata tcccagctcc aaatgctata atatttttta atgctcctcc
     1381 aagttctact ccaactatgt ctggattcgt ataaactcta agtttagggc tcataaataa
     1441 atcttgtatc atctgtgcta tttttaaatc tttagaagca actactacgg tagtaggaat
     1501 atcccttgct acttcttcag catgagatgg cccagatagt attacatatg gattttgtgg
     1561 taattcttct tcacagactt gagataatct aagcccagtc cctttttcaa gacctttagc
     1621 tacattaaca ataacttgtc cttcttttat aaatggtttt attttttggc aagtacttct
     1681 tacagcctgt gatggaactg ctaaaactat taccttacaa tcttttatag ttttttctat
     1741 atcagtactt atagttatat tatttggaaa taatactcct ggtaaatagt ctgtattttc
     1801 tttagttctg tttatttttt tagcttgttc ctcttttcga gtccacatgc tcacatcatt
     1861 accttttttt gcaagcccta aagctaatgc acttccccag cttccagtac ctaatacaca
     1921 tactttctcc atgtttaaca ccacctgagt ataatatatt tactattctt tcttttgacc
     1981 aatttttctc tctgtcccat ttaataatcg ctttatattt gctctgtgat tatatattac
     2041 tgatgcagtt aagaacagag ctactattaa accagcttta ttttttacca taatcataaa
     2101 tattggtgaa catcctattc ctacaacaga acccaatgaa acatactttg ttatagctac
     2161 caccaatata aatactgcta gacacattag tgttattact ggatttacag ctagcattgc
     2221 ccctagtgaa gttgcaacac ctttgcctcc tctaaatcct aaaaccgctg gccaattatg
     2281 acctgctaca acacatataa ctgccagata tccagctaaa agagtatcta ttccaaccaa
     2341 tcttgctgca aattctgata ttaaaacagc cattacacct ttaagaacat cacctaaaaa
     2401 tgtcattgct cctgctcttt ttccaagtgt cctaagtaca tttgtcgaac ctgcattacc
     2461 agaaccttga gtccttatat caaccccagc aattcgcttt gcaactatgt atgaggtaga
     2521 tatatttcct aaaagatatg caactacagc aataattata taactaaata tctccactga
     2581 atttcccccc tttagttata ttttaagata tttatttttt atttttttgt ctatactcaa
     2641 actttatgga agttccttca aatccaaaat tttctcttat cttattctca aggtatcttt
     2701 gatatgagaa gtgagttaaa tccttatcat ttataaacaa tgtaatttta ggaggtctaa
     2761 tgtcagtttg agtcccatag taaattttta atcttctacc tttgtcagat ggtggttgat
     2821 ttaacattac agcttctcca attacttcat tcagtgctga agttgatatt ctcttagaat
     2881 gttcatttga cacatattca actgtatcta gtatcttgtt cattctttga ttagtttttg
     2941 cagaaacaaa tacgattgga gcatacatca taaatggaaa tttttctttt atatcttttg
     3001 tgtaattgct catagtctta ttatctttct ctattaaatc ccatttattg attacaaata
     3061 tgcatccttt tccttcatca tgtgctatac ctgcaacctt tgtatcttgt tctgttacac
     3121 cttctaaggc atctattact atcaatacta catctgctct atctacagca ctcatagccc
     3181 ttattacact gtatttttct attgtctcat atattttact ttttcttcta agccctgctg
     3241 tatctattag aagaaatttc tgtccatttt tttcaaaata agtatcaatt gcgtctctag
     3301 tagttcctgc aattggactt acaattactc tttcttctcc aagtatctta ttgagtatag
     3361 aactttttcc tgcatttggt ttacctgtta tagctactct tattatatct tcttcatact
     3421 ctgtatctaa tcctgctgga aaattttcaa ctatctcatc taataaatct ccaaatccca
     3481 tactattggc tcctgaaact gcaaaaggcg ttccaaatcc caactcataa aaatcgtata
     3541 tattatcaaa ttgactttga ctatcaatct tattaaccac taagattact ggtttttttg
     3601 tctttctaag caattgtgca atttctttat cagcagctgt tattccagct ttaccatcaa
     3661 ctataaaaag tattacatgt gacatgtcca ttgcaagcat ggcttgattt ctcatttgag
     3721 ataatattat atcttcgcta tcaggttcaa ttcctcctgt atccactaat gtaaaatatt
     3781 tatctaacca ttctacttca gcaaatattc tatctcttgt aactccaggt gtattttcaa
     3841 ctattgatat cctttttcct gcaaatttat taaatattgt agacttacca acgtttggtc
     3901 tcccaactac agctacaact ggcctactta tactcatttt atttctcctt ccatcttttg
     3961 ttttctattt taaaatttta tccacaaaat cttttccttc attcaaacaa gggataattt
     4021 ttattctcat aagttcttct aattgattta attcaatatt atctaagaaa atttcttcat
     4081 ctgcttttag catacttctt gttatataaa gagcttctcc taattcttta tcttcaagtt
     4141 gttcttttaa atccttagct gttaaaagac ctgatacagt tattgtctct ccaaaaaact
     4201 tgtttttgat ttcatataca tttacttgaa gatttttaaa cttgtccatc atagcatctg
     4261 ccatactctg tataaattca tatgcagaat gaccagtagc tattgatact ttcttctctc
     4321 tttttagaat cttctcaggc aatatattta gatactcttc aatctctgtt tttaacttac
     4381 gtatcatacc tacaccatct tcaaactgta aaaaaccttc atattcttca tatccaggca
     4441 attcactatt agatagtata taaaattcat ctgataaaaa tgcaaatcga gtacctaatt
     4501 tttcaagata tttctgttgt atttgatgta tttgctctat agttttactt gcgctcttat
     4561 cattaaatat atttaattct actagatttt ctctatgctt tgtaatccca actggaacta
     4621 tcgcaacact gtttacatac ggataaagtt gagctagttc ttttattgtt ctatccaatt
     4681 cctttccatc atttatacct ggacataaaa caatttgaca gttcatttct atatgagcat
     4741 ctgccaatct cttcattata ccatataatt ttcctgcaaa tttattgctt atcatttttt
     4801 gtcttaactc tggattagtt gtatgcacag atatatttat aggacttatt ctatatttta
     4861 taatattatt tatatcttct tcactcatat ttgtaagtgt gacaaaattt ccttgtaaaa
     4921 aagataatct cgaatcatca tctttaaaat aaagagtttc tctcatacct tctggcaatt
     4981 ggtctataaa acaaaacata cacttatttc tacagctttt agcttggtca attatcggat
     5041 ttgtaaattc aacacccaaa tcttcatcat agtctttttc tatctcgtat atgtagactt
     5101 cgccatcttt tttttgtatt tctacatcta aatattcatc acttagtaag aatctatact
     5161 ctattatatc atgtattggc tgttcattca cagatataag taaatctcct acctctatac
     5221 ccatttcttc tgcaatacta tctttataaa ccttacttat aatattattt acttttttta
     5281 catttacttc caatattttc accactcttt tgtattcatc aatgcttatt ataatgtatt
     5341 ttaatatgca atttatttat ttattatatt ttatgatagc atttttaagc atttcatgtc
     5401 ataataacac attttaattc taatagtcaa tataagtaaa tagctataaa atagattaga
     5461 acaaataata ttcaaattta agtttcttaa aaatttaaat attatttgtt cttaaaaaaa
     5521 ttttataaat ttaatgtttt actatgatta acgtcccatt tgtcatatca tgaaccattc
     5581 cacctacaca ctttgacatc aacaatgcct tattttgtaa gtctttctca tcatttgcat
     5641 aaaatattgg acaacctcca ccatttttca tattcttatc aagtgtaact atagctaggg
     5701 tatattcatt taaacctata tccattccca tctcatgcac ttcctttctt tcctatttta
     5761 taatttttta gtgataagtt ttttccttta gagcttgaaa gtattgggca tgactttaca
     5821 gcttccatta ctttttccac attcttatct actggtaaat aagcaatcat tatactttca
     5881 tcctctttat ttcttctagg taatggtacg aatgaaggtt ctccataatc tccatataaa
     5941 cctaaccttg aatatatatt gtatactatt gcttgtcttt gaccggagtc aaataatatt
     6001 cctgcattag cataatcctt atttttagga attatttgta ttcctacacc tctattaata
     6061 tatacttctc tatcgctttt tagacctata tttgttatac cttttaaatc tcccaccttt
     6121 agtatagatt cgtccacaaa actaatttca gcaggcatta catcagctat gtctccaata
     6181 ctttttctag taagaagttt ttttaatata aaggctaaca ctaatccaca aattgaactt
     6241 attaccaaac tcatcccttc acttattttc gtttctgcaa gtattacata atacaatcct
     6301 attgttaaaa aagatgtaac tatacacata taatttctta cttcataggt tctagctatc
     6361 tcttctatga atgcagctcc tctctttaca agttgaactt cctctaaatt ttgcaatgta
     6421 tttcttctat ttgctctcac ttgtctaaat tgttctgctg ctagagataa aaaagttata
     6481 gatgtataag aacgatttat aagagctgga accaataatg ctcctaatga agaagcgacg
     6541 aatgctaata taagttgaga tagtaaaaca tttggttgcg ttggatactg tttttggtct
     6601 aaattcaaca ttataattct agatgttata ccaataataa cagcaacaaa aaatactcta
     6661 tccattaaat cactcctaaa atatttaatt tgaaatttta catgtaaatc tacaaagtct
     6721 ttctagaaaa atcactctgc tttccttttg atgtttctat gataggtgta cttttcacta
     6781 catctatcat gacatctata tctttgagta ttggcatata tggtataacc acagtacttt
     6841 tttctaaatt ggtcttagat atagcaacaa tatcaacctc atctacatct ctatctatcc
     6901 ccatatgaat aaatagatta tgtattatag cttgtctttg ccctatatca tttactattc
     6961 caaatgcacc taaatctctt ggtataactt ctatggccag acattcattt ctatactttt
     7021 ctcttgtact ttctagacct atgttagtta ttattacacc atttaccatt aaaataggtc
     7081 cttcaaaact tatttttgca ggaactatat ctgctatatc tgctatactg ttccttctta
     7141 aaaatcttct aaaaatcaaa ccaacaatag ctcctgacac aatagctagg attgtgcaat
     7201 aaaaaaagct aaggccatac tttcttgcaa aaacgatata aactatcgaa gataccagtg
     7261 cagaaaaaag acttatataa cttctagatt catacgtaga tgctatttct tcaacgtaag
     7321 cgtctccttt tggtactaat tcttcattat cgatattctt tagtgttatt ctttcttgtt
     7381 ctgcaagacc ttgaaactgt tgtattgcta ctgcaaaaaa tgtcaaggct gcaaactcct
     7441 tatctataag tgcaggtaat gctatagcac ctaacgaagc tgaaagccca gatataatta
     7501 tctgttctaa ataatcttgt ggtcttgatg ggtattgttt gtctgtaact cttaaaacta
     7561 atcctctaca taatattcct acaactaatg caactataaa agagtgtcta aataaatttg
     7621 cattcaataa actttcttcc attttctcag ctccttctct tatatagttt gtgcaaaaaa
     7681 caaaaaaaat tcccttctat ttaaggaaat ttttttgtaa tattgtaact ataaattcgc
     7741 taccaatgcc aagttcactt tttactttta tacatccatt tagtgatttg actatatgct
     7801 ttgttatagc caatcctaaa cccgttcctc ctacatccct acttcttgct ttatctaccc
     7861 tataaaatcg ttcaaatatg cgttctacat cttcttttgg tatccctaca ccattgtcta
     7921 taactttaat tacgattttt tcttcatctt ttaacactac aacttgtaca atcccattat
     7981 cttgggtata ctttatagca ttatcaacta ggtttaagaa cacttgcttt atatagtctt
     8041 tattggatag tatacttatt gatttatcat cacatttata taaaacttct atattttttt
     8101 cactagctga atgttgagtt atttcatata cctctttaaa tacatcatat actacaacat
     8161 tttccataag aagagtatct ttattttcta caaatgataa cagcaatata tcatcaatca
     8221 acctttttaa tctatctgat tcgctttcta ttatttctaa gaatctattt ctagtattca
     8281 catctatatt ttcatttgac tttagtgttt caacaaaccc acttatagat gtaagtggtg
     8341 ttttaagttc atgacttaca tttgcaacaa aatcacttct catattttca agtttaactt
     8401 ttttagtaat atcttctata tttactatcg aacctattat tacgttgtta gtattctgaa
     8461 gataaactgg gtctactttt atcttatata cgatatcatc tttagtagta atattagcag
     8521 tttcattatc tttagaacca ataaattgaa gtatttgttt taatatttct tcttctttta
     8581 ttacaacatt aatattatga ccttcaacta attgatttgg ttcactcttt atcatttctt
     8641 ttgcttcatc atttatcaga agtatatttc catttacatc tatagccaaa attccatgag
     8701 atatactctt taatatagac cttaactgta aatgtttata ctgtacttct tctaaggttt
     8761 tatccatatt ctttatcata tgattaaaat ttctagctaa ttcgccaagt tctcctttgg
     8821 ctgatatgtt aagcctagca tgatattcat tattacttac tttttttgag acgtatataa
     8881 actcttttaa atatttcctt aatgttattg tgtatcttac agctaatata acaatggcta
     8941 tggacaacat aacaataaaa aaatatatac tatttaaatc ttccacaaga taacactcct
     9001 aaaatataac ctttttaatc tattttgtat cctacacctc ttatagtctc tatatatttt
     9061 tccgatttat cttcatcttc tatttttttt cttaaatatc ttatatgaac atctactgtt
     9121 ctagtttctc catagtattc atacccccat attttatcca aaagataatt tcttgaaagt
     9181 acttttcctt tattttgaat taataatttt aacaattcaa attccttaag agttaactct
     9241 atcttttcag agcctttggt aacttcatgt tttgataaat ctagttttaa attacctgta
     9301 gtaagtatat tgttttcttc acctaagcta gtcaaattat atctccttaa aacagaacta
     9361 atcctagcta acaactcttt tatactaaat ggcttagtta tatagtcatc agcacctatt
     9421 tcaagccctt caaccttgtc attttccata tttttagcag taagcattat aacaggaata
     9481 ttttttaagt ccttatcact tcttattttt ctcaaaactt ctattccact tatatttggt
     9541 aacatccaat ctaaaagtat taaatctggt ttaatttcct tagcctttat aaatccgtca
     9601 aatccatcat atgaataact aacttcgtaa tttgacacct ccaaattgaa ttttaatagc
     9661 tcaactatat gcatttcatc gtctataaca agcactttag tattcattaa aaagctcctc
     9721 tctattttat ctgtagtacc gttcctattc gttctgatgc cttattatgt tttctataag
     9781 agtggaattt atctgctcta caactagtgc ataaatttaa atttattata ttttcttttt
     9841 taactccact acatctaagc atatactcat taattttcca taaatctaaa tagtaacttc
     9901 cctcttttat tatatagaac ttttcatcat tatttgtaag aattgtgtta aatttttcta
     9961 ctaaatcttc agacacttca taacaacatg gtccaattga tggcccaatt atacatacta
    10021 aatcttcagg aactgtttta tagcattcag acattaattt tattgtttta tgacctatat
    10081 tactaaaagt ccctctccaa cctgcatgag ccagtcctat ggctttattt ttagcatcta
    10141 taattgatat aggtacacag tctgctgtaa acagtaaaag tggtactttt tccaagtttg
    10201 ttataagtgc atctccatct cttcttttcc ctatagtatc tttattgact atattaacta
    10261 tatctgaatg tatttgagaa ttactagtta gattttcaaa tgtcaaatca cattctttac
    10321 atacttttaa catatcttct ctattttttg catctatatt cttacatgtt ataactagat
    10381 ttacagaatt taatttttca tcaagttctt cacaattacc acttaaatta acatactctt
    10441 taatatcttg tacatttttt atatttatat aatctttcat catttgtctc ctttttctat
    10501 cagtatactt ttaagttcat ttacaaaagt atttatatct ttaaattgtc tatacacaga
    10561 agcaaatctt acataggcaa cctcatctaa ctctttcaat ttatccatta ccatctcacc
    10621 gatttttttg gtttcaattt cttctataaa acatttattt atttcatttt caatatgtac
    10681 aacaatactt tcaatctcgt caatagaaac aggtcttttt tcacatgctt ttagcaatcc
    10741 atattttatc ttttctctat caaaatattc tctactatta tcctttttaa tgaccattaa
    10801 aggagttgtt tctattttct catacgtagt aaacctcttt ttacatgatt cacactcacg
    10861 tcttcttctg atagatttta aatctgtatg tctagaatct attacctttg attctttata
    10921 attacaataa ggacattgca tctatattcc tcctttgttt aactatatct caaactcatc
    10981 taagcttaat tctgaaccta tatttactat tattgtatca caacctattt taagtatatc
    11041 attccaaaat ataacttctt catcccctcc tcttgaaaaa aaacttctac ttccatctcc
    11101 aaaaatggta aatgagacta ccttaccttc gtgaatatcc atgtctatgt ctattataaa
    11161 ccccatcctt tttccatttt ttacatttat aatttctttt tccattatat cagaaactct
    11221 aatcatattg tactcaccct ttctttaaaa ataaatatgt atcgaagtaa aaaaaaatga
    11281 cctatatagg tcatttaaat taaaataatg tttacagtcc atggattact tatacatatt
    11341 ttctcatatt ttttaaagca tttttttcaa ttcttgatac ttgtgcttga gatataccga
    11401 tttcatcagc aacttctatc tgagttcttc ctttgtaaaa ccttaaatct aataccaact
    11461 tttctctact atttaatttc ttaatagctt ctttaagtga tatttcttgc aaccaatttt
    11521 catcagtatc ttttttatct tgaacctgat ccataacgaa tattgcatct ccattgtctt
    11581 gatatactgg gtcaaacaga gatattgggt cttgtatggc atcaagtgcc attacaactg
    11641 attctacttc taattctaat tcctttgcta tttctgatac tgtaggttct ttagaatttg
    11701 ttctaattaa tctttctcta acttgtagtg ccttatatgc tatatctttt aatgacctac
    11761 ttactctaat tgggttattg tctcttagat accttctaat ttctcctata atcataggaa
    11821 cagcataagt tgaaaacctt acattttggc ttaaatcaaa gttatctata gcttttataa
    11881 gaccaataca acctatttga aataaatcgt ctatattttc tcctctgttg ttaaactttt
    11941 ttattacact caagactaat cttaaattcc ctcttacaaa ctgttgtctg gcttcttcat
    12001 caccattttt tatttgtaat aaaagttcct tcatttgctt atttttaaga actggaagtt
    12061 ctgatgtatt aacaccacat atttcaacct tattaacttg catatatttt cagtcctctc
    12121 tcaaaagtat tcatctattg aaattattta catttccaat ttttttatac tttttaagaa
    12181 gaattaattt agcattatac aaattttttc atttcttttt gcaaccttga gattattttc
    12241 ttttcaagtc tagatatgta ggactgtgat attccaagca ttccagcaac ttctttttgt
    12301 gttttttcaa tacctttatc aataagacca aatcttaatt ccataatttg cttttctctg
    12361 tcacttaatc tgtcaagtgc cataaccaac aaatctctat ctatttcttc ttcaattatt
    12421 ttgtatattt catcattttc tgtacccaat acatcagata ataaaagttc gtttccatct
    12481 aaatctatat ttaaaggttc atcaaatgat acttctgttt tctttttatt attttttctt
    12541 aagtacatta atatttcatt ttctatacat ctcgaagcat aagttgccaa ttttatattt
    12601 ttattcagtt taaaagtatt aactgctttt atcaaaccta tagttcctat tgaaatcaaa
    12661 tcttctacat ctatacctgt attttcaaat tttctcgata tgtagactac taatctaaga
    12721 tttctttcta ttaaaattga ctttacactt tcatctgttt ctagcttttg taaaagctcc
    12781 atttcttctt ctggtttcaa tggaggagga agtatatttg cccctcccat atagtatatt
    12841 ccctttggct ttattatttt taatttaata cctaacatag taataaagct tattattctt
    12901 tctttcaatc gtaacattcc ttgtccccct taaattttca cctaaaatcc aggcaatata
    12961 tttggattca ttattgctcc atattctcta tcattaaaat ttgaaatacc aagtattata
    13021 tttcctatct tttgctcatc tacttctata tagtcagctt ttaagcctat tattatgctt
    13081 gtcttattac ttccagcatg tttataaggt attattctaa ctctacctga agttttatca
    13141 tctaaacttc ctataatttc ttgtgctttc ttcatatcta tatcttcata attaatattt
    13201 aatagttttt ctggaataaa tccttctaac acactagact taactattat tacatcactt
    13261 tgacttattg ggtcttttaa caaattacca ctatctacta aagccttaca gcaaaaactt
    13321 ttttctagta agtttatatt tatagtcttt gtaagctcct taatgtactt aagcattttc
    13381 aaatctttat aaatgtattt taataattca caactcacat aagtacatgc aattaagaaa
    13441 gatattttta agtgacttat tcctgtaaaa taaattataa aataagtgct tccagaaata
    13501 aaaatgttta ctagataaaa aactaacgct acatgaacat aatctttttt gctcttactt
    13561 ctaaatgaaa ttaaagttat tatactcatg attattattt taaatggtac agtaaatagt
    13621 atattcattg atggatacaa ataagcaaca gaataggcac ctcctaaact agcccccaat
    13681 aacttatttt ttttaggatt atatctttta gtaagaatag aagtacaact tataataata
    13741 taatttatta ctaggttttc tataacatag tactcaatat acaccatgta tcccccctta
    13801 aaacctttaa acatattata agatagtatg tacaaaattt tatgtcactt ttgatggtcg
    13861 aatccaaaac aaattctatt taaaaagaac aatgatatat gtcttatact ttatgtcgtc
    13921 aaaagatatt tttttaatac caaacaaata gacttttttg tagaatccaa tatatctagg
    13981 tagaagttat tcactctact tttggtatat ttacaaaaaa agactacata atttaataat
    14041 atatcaagta aatcttttac ttatataaaa tcaaattata tagtctgtaa attcaaatat
    14101 aaattattgt gctagtaatc catttgcata ttttgcagat tcatttagtg cttgttctgg
    14161 agttatgtct ccagctatca tcatttgaag ttgtttccat aaatattcat atatctctac
    14221 tccatgaggt ccaactgtca atggtctata catatgttta tctcttgtca ccatttcttc
    14281 aaatcttggg tctccttggt atttttcacc tttagcaact ggagctctag gtatttcttt
    14341 gtggaaagct tccattgatt ctttgcttag catatactca agaagtttaa atccaagttc
    14401 tttattttta catgctgaca ttaatgttaa atcatctact gaagcaaaac ttccaactgt
    14461 cttatcttct aatgatgtta catatcccca gtttaaatcc gggaatgatt tatcaaaagt
    14521 acttgtagca gcagaggata gcattatagt aaatgcagct tttcctggtc caaatacact
    14581 ttcaatcatt tcattactat cttttgataa agcatcttct ggaatatact ttttcatatc
    14641 atttataaag tttgcagctt tcaagccttc tgcattatta aatttaactg attttaaatc
    14701 atcattatat atatctccac cagcttgcca taaatacgga taccagttcc agtttaagct
    14761 tccaaatact ttagaacccc aaccttgtgc aagaccccac tggtcgattt ttccatctcc
    14821 atcagtatct tttgttgctt tttcacagat tcttttaaaa tcatcccatg tttttggagg
    14881 tttttctcct aatttttcta gtatatcttt attgtagtac aatacgcctg gattagcagc
    14941 ttctataggt aaaccataaa taccacccat catttttgca tcatctaagt atagataatt
    15001 atctgaagtt cctgattttt ctaaataagg tgttaaatct tctaccgcac ccatttctat
    15061 aaattgagga aacatctctg catacatgta tcctatatct ggaccttcac ctgcacttat
    15121 tgctgttgca tatttttctg cgtagttatc ccatggtact atctgaaatt caatttcaca
    15181 gttattttct ttttcaaatt tttcaaatat cggcttccat gcatctgcat catggtctgt
    15241 taatggtgca gaccatacaa ctaatttttc tttaccctct ttgcctttag aatcttcttt
    15301 ttttgaacat cctgcaagaa ttgatactga tagaatccct accataacta aagaaagaac
    15361 cttttttagt ttaatcatgc ttaatcccct ttcagacttt aatttgtctt tttcctcaaa
    15421 tgtttacatt taattcagat ttaatattaa caaataagat tactcttgtc taatcataaa
    15481 aaactatact aactcctata actatatttt ttttctatac ctattagatt taattcatac
    15541 taaaaactta ctttttaaat tttatcaata tttaaaaatt tttttaagtc tagccacaaa
    15601 ctaactatct aaattgagca aaataaaaag aagtctattg acttctttat aagcttacat
    15661 attatcttct tcttcttagg aatgtaggta tctccatgct ctcatcatca tcttcttcaa
    15721 ttatacttgc ctttttagga ggctctattt tttcctcagc aattacttct tcttcaattt
    15781 cttttacagc ttgttttact gttgtattta atgaagaacg tatgcttgat ttaggtttag
    15841 tatctaaatc taaggcacca ttttgtaatc cttcaaatcc tgtcgcgatt actgtaatca
    15901 ttatttcatc acctaaatct tcttttatag aagctccaaa tataacattt gcttctgggt
    15961 cacaggattc cataactaaa gttgatgcct cattaacttc aaataatcct aagttaggac
    16021 cacctgtaac attaagaagt actcctttag ctccttgtat tgatgtttct agaagtggag
    16081 attgaatagc ttgtcttgca gcatcaattg ctctagtttc accactagca ctacctatac
    16141 ccatatgagc aagtcccttg tctttcatta tagtagtaac atctgcaaag tccaagttta
    16201 ttaacccttc aactgctata aggtctgaaa ttgattgtat accttgtttt aatacatcat
    16261 ctgcaactgc aaaagcatct aacattgatg tatttttttg tactatctgt aaaagtctat
    16321 catttggtat cgttataaga gtatctactt ttgatttaag ttctgctatt ccaccttcag
    16381 cgttcttcat tcttatctta ccttcaaatg caaaaggttt ggtaactaca cctactgtaa
    16441 gtatccccat ttcttttgcc aagcctgcaa ccactggagc agctcctgta ccagtaccac
    16501 cgcccattcc ggctgttaca aagaccatat cagctccttg aagtaacttt actatttcat
    16561 ctttactctc ctcagcagct cttttcccta cttctggatt tgctcctgct ccaagtcctc
    16621 tagttaattt ttctcctatt tgaactttat attcagcttt tgatgtatat aaagcttgtt
    16681 tatctgtatt tactgatata aattcaacgc cttttagttg agcttctacc attctattga
    16741 cagcattgtt tccaccgcca cctacaccta ttactttaat ttgtgcacat tcttctaatt
    16801 ctacgtcaaa gtttagcatc ataaaccccc ttctctcatg attcaaagtt atcgtatgta
    16861 ttaattatat cattttcttc ttttattttg ttaaccttta agtaacatat tccacaaaaa
    16921 cgtaaatttt cctttttaaa tttcttactt atattgtcaa atattactct ttaagtataa
    16981 aataatttac gtatatatta aatgctcttt aatttttacg ttttttgttc ataatattac
    17041 taataatata tcgtctaatt atagataagt tattaaacaa tctcatacca aaaactaata
    17101 cagcaacata gtaaagagaa actcctaact tttctcctat ataagtcaat aatatagcca
    17161 atatagcatt acctatgaaa ccagtaataa aaattacatt atcatagtta tcttcaaaat
    17221 tggattttat cgctccaaaa atagaatcca ttattgcaag aatagctact gacatatata
    17281 tggaataatc catagaatat gtaaatggaa tatagtaacc tactactaca ccaaatgcta
    17341 atcctcctaa aacccataac ataaatcttc acctctttat tttactttga aacacaacta
    17401 cattacaatc ctttttatta atttttttta ctttttcttt atttttttgt atttttcgtt
    17461 ttattttgca aataaatcca ctttatttcc attattttcc tgaactttta tattaattcc
    17521 atatatatcc ttaagagatt tagcatatga ttctggagag tttatagctg cactcaataa
    17581 atctatatct cctatagctt ttattataaa tggttgtcca tatgtagtat catttactgt
    17641 aatagtagct cctgaacatt ttatcttact tgttgctagt actctttctc catttataga
    17701 aattgctctt gctccagcct tttttaaatc atttaaaacc ctcaaaatat ctatatcatg
    17761 tataatcaaa tcgtttggat tctgtccgtc ttttaactct ctctcactat ccctcatagt
    17821 aatagttatg cctggccctg taacagttga ataaccactt aattctttaa cttcttcaag
    17881 ctctttttgc ataagtgttt ggatagattc atttccattc ttttttgctt ctttatattc
    17941 attcaaagtt tttttgttgt ttttaagagt ttcttttaaa ttttttattt ctttttttgt
    18001 attttctacc tcactttcaa tactttcagt ttgacctaat gttatgtata ctctatttgg
    18061 gtctagtgat tttatataag ttgatatgaa aacgcctaat atgaatgctc ctaatataac
    18121 tattttcttt ttcatactat tatttcccct ctgctttgtt tatatcacta tgaccattta
    18181 tttctatatt gtcttctttg gtcactttta ctttggcatt ataatatgtt tctatttccc
    18241 agacaatacc atactttata ttaagtgccg aagccaatgt atcagaatca ccaattgctt
    18301 ttattataat tggttctttt atgacagtgt cattcatata aagcttgtct tcttttaaat
    18361 acatatatgt attagaaact attctctggt cgtttataga tatggcactt gcttgtgctg
    18421 aatttaattt atttatcatt gacaataata aatcataatt atatagtata ctatcatttt
    18481 tatcagagtt tcctaagtct tttgtagaat ttatctcaat ttttatgcct gttccttcaa
    18541 catctgtata tccagctaat gtttcatatt tttctatttc tgacttaagt atgtcttgtt
    18601 ctccttcatt gtttttgcct ctataagtct ctatatcttt cttcacacta ttaacttctg
    18661 attgtagatt ctctttttct ttttttaaag atttaagttc ttctgtaagt tgctctcctc
    18721 ttttaaaagt tgtcatccct ctattttcta tattaataat tttaagttgt aaactaacaa
    18781 gaacacccaa tacaatactt cctatgatta tacttttatt tataaatctt tttttcaata
    18841 tatcacttcc ttacaattgt ctatccttct ggactataca ctgccaactt tccatagtta
    18901 agatttatag tccctccatt agtattttta ttttgcaggt caatcagaat tttacttacc
    18961 ctagatatat tataatctaa gttatcatca ttagaaagta agatttttat attacttctt
    19021 gtaagcattt ctatattgct ttcttttttt aagtttacat aatttatttt tctataaatt
    19081 ccttcttctt ttaaataatt taaagtttta aatacattct ccttagtttt ataattttta
    19141 aattttatag atttattatc atcaatgcta taatctacat ctactatcaa gtctttttta
    19201 ctttcattag aacctcttag ttcttccaat aaatttcctt ttttatctat ataacaataa
    19261 ttatcttcat cttttaatac tgcaaatatt tctttttctt ttaaacttat aattatctta
    19321 ttgggaagtt ttctttttat ttccacattt tctatatatg gattttttat aagtttattt
    19381 ttcatatctt taaaattgta tgcaaaaata ttttcattta gatttacatt tagttctttc
    19441 attatatttg atttggtcac cctcttattt ccaattacat ctattttctt tacatcaaat
    19501 aaatcacttt ttacaattat acatatacaa aaagataaca taagtataaa aactaaaact
    19561 atcatgacat tattcgtatt taactttctt ctttttttca aatataatca acccctttgg
    19621 ttaataaata cacctttaga gcttgttttt cctacaaaaa aattataata tttaaagtca
    19681 aatttttgca ttttattaca taacatccaa ataaggtatt aaattttata aataaatcaa
    19741 tatttcatca aaataaacat agcatactac atactattta tatatttaaa tctgtataca
    19801 tacaaataaa cccctaattt ataataattt agaaaatcta atatattata aattaggggt
    19861 aaatatttac ttatcttttc tttattccta ttaccttagc ttgaccttct atacttggag
    19921 tcgtttcttt ttttatttct tttacttcct taataacttt ttcttttttt gtttttttag
    19981 aagttgactt ttgtgttgaa ttgtatactt tcattatctc atcatatata aggtctatgg
    20041 cttcaggctt acctatagtt ttacttgcat ttgccatatc aaccaaaagt tctctatctc
    20101 ctaaaagttt aaatactgct gtatttaggc tctctggagt tagatttttt tctaaaatag
    20161 caattcctgc cccttgtttt tctatactct tggcattata ctcttgatga ttttcagcag
    20221 tataagcttt aggtattatt atagatggct ttccaagagc cgttatctca gctaatgaaa
    20281 ttgctcctgc gctacctata accaaatcac ttgctgcaag tgcatttgcc atatcttcta
    20341 agtaaggcac aactttttgg tatggtttaa gatttatatc gctaatactt cccataaatt
    20401 catcataata gcttttgcct gtagcaaata taaaagctat atcttcattt accatattct
    20461 ttattacaag tctcattgca tcatttattt ttcttgaacc tccactacca ccatagcaaa
    20521 gtaccattct tttttcgtca cttattgata gattctttct tgcaatattt tttcttgata
    20581 ataaaatttc ttttctaact ggatttcctg taaaaactaa tttatcttct gctgcctctg
    20641 gaaatctttt atgcgaatct tcaaaacttg taagtacctt tgtaactgtt ttagataaaa
    20701 tcttattagt tacaccagga aaagaatttt gctcatgtat tatggctggt attttcccca
    20761 tagatgcatt aaataaaaca ggtccactaa catatccacc agttcctata actatatctg
    20821 gtttaaattt cttaacaatc tttcttgatt gctccaaacc tttaaaaagc ttaaaaactc
    20881 tttttacatt atcgaaatct atttttcgct taaaaccttg aactgttaca gtctttaatt
    20941 caaatccata cttaggaact atttctgact ctattccctt ttccgtccca acaaaaatta
    21001 tctcagcgtc tggatgctca tctcttattt tgtttgcaat agctatagct ggatacacgt
    21061 gccctccagt tccaccgcct gataataaaa ctttcatcaa atcatcatct cctgttaatt
    21121 aattttgaca tgctttgaga tgtttaacac tattcctaca gctcccataa agatggtgag
    21181 tgaagttcct ccataactta taaatggtag tgctacacct gtagctggca ttgatgatgt
    21241 cgcaactgct atatttaaag ctgcttgtat tccaatttgt gcacctatac ctataacaac
    21301 catacaagca aatacattag aagtcttcaa agctattctt acacatctgt agactaaaac
    21361 tacaaatagc ataataacta ttatacaacc aatcaatcct aactcttctc ctattatagc
    21421 aaatataaag tcattttgtg gctctggtat ataaaaatac ttttgtttac tctttcctaa
    21481 gcctagacca aacaatccac ctgaacctag tgcatataga ccttgtatta cctgataccc
    21541 cttaccaaga gggtcttgaa aagggtctaa aaatgatgta acacgactaa gtctataggg
    21601 ttcagcaatt actaatgcag caaataaagc agccccactc cctatcatag caaatacaat
    21661 tttcatatcc attccagcaa caaatatcat tacaaaagta actagaatta cagtacctgc
    21721 tgttgacaaa tttggctgaa gtattatcaa cgcaaaaaat atccctggaa ctacgagaag
    21781 tggtacaaca ccttttgtca aagattttat tttatcataa tttttttcaa ttaattttgc
    21841 agttaatatt attgtagcaa actttgcgat ttctgcaggt tgaaatgtta aagctcctat
    21901 tcccaaccat ctttttgctc cgtttgcttc tatccctaaa ggggtcaaaa ctaacaataa
    21961 taatactact gctatagctc caattgctgt agcatttttc ttccaaaaac tataatctat
    22021 tcttgatgta ataatcataa ctataaatcc aagtacagca tatattacat tcttttttag
    22081 aaaatagtac gcatcattgt gtttaaatga agattgtata aaacttgcac taaacaccat
    22141 aactattcct acaaatacta aaagcatagt agtgtaaaac actactccat caaattcagt
    22201 cttcaccctt atgtcaattt gttttttcaa attttcttta ggcatccaac taatctacct
    22261 ccattcttcc atttaagtaa gtttagaggt aagagattat tttaaattgt ttacattatc
    22321 tttgaagtct tttcctctta cttcaaaact ttcatacata tcccaacttg cacaagctgg
    22381 tgaaagaagc actatatctc cactttttga aatttcataa gaagtcttaa ctgcttcttc
    22441 catatctttt actatatgta tgtcattaaa tcctttattt ttagcacaat tttctatatt
    22501 tgatgcagtt tctcctaata agaccaatga tttaacatat gactttgcag tttcaagaag
    22561 ttcatcaaaa ttacttccct tatccatccc ccctgctata agtattatag gtctatcata
    22621 agactgtata gcttttatag tggaatctgg attagttccc tttgaatcat ttacatatat
    22681 tacattgtct aaatttctta caaactcttg tctgtgttcc actcctttaa atgtcataag
    22741 tacatccttt ataacttcta aatcaatttt gcagacatat gctattgcta tagctgccat
    22801 acaattttct aagttatgac ctccaggtaa acttacatca tctttattta gaaatttgat
    22861 tttatcatct atatctatta ttatgtcatt attttctaaa tatactcctc catttacttt
    22921 tttagttctt gaaaagtaaa ttactttggc attgcattta tcagctaaag attttactat
    22981 ctcatcatca tagtttaata cacagaaatc ctcaacagtt tggttcataa atatatttgc
    23041 ctttgcttct atgtattttt ccatagtatg gtgtctattt aaatggtcct ctgtaatatt
    23101 taatattgcg cttactttag gtctaaattc atctatactc tcaagctgaa aactgcttag
    23161 ttcagttact aacacagact cttcacttga agtttctata gtatctatta caggatttcc
    23221 tatattacca actacatatg tatctcgttt tgctcttgaa aaaatttctc ctaccaaact
    23281 agttgtagtt gtctttccat ttgtacctgt aatccctata aaaattggat tatccgatag
    23341 tttaaaagct aattctacct caccaattat atacttgcct gaatttttta atttcaatat
    23401 gaatggtagg tctagtggta cacctggaga cactacaacc atatctatat catctacatc
    23461 ttctgggtga tgtcctaata tgtattttat gtcatttata gatttcaatt catcaagtat
    23521 atttctcaat ttattttcat cttttatatc atttacgatt atacttgcac ccaatttatc
    23581 taaatgttta attgtagata taccagtttt tgcaagtcct actaataaaa ctttttttcc
    23641 ttttaagtcc atttttcatt ccccctagcc ctaaaatact gctattatac ttatccaagc
    23701 taaaacaact gttataatcc aaaaaataaa tacaactctt gtttctggcc aaccacactg
    23761 ttcaaagtgg tggtgtattg gagccatctt aaagaatctc tttcttgttg ccttaaaata
    23821 tccaacttga atcaaaactg atagagcctc cgcaaaataa attcctccaa ttataggtat
    23881 tataagcact gagttagtta gaactgaaaa tgctacaact gcacctccta atgccattga
    23941 acctgtgtcg cccataaaca ctctagccgg atatgaatta aatcctaaga aacctaaaca
    24001 tgctccaaca gttgctgctg ctaaaactgc gacttctgta tttccagcaa tagaacttgc
    24061 aaatagcata aagaatacag aaactattaa tgttactcct gatgctaatc catctaatcc
    24121 atcagtcaag tttacagcat ttactattgc tacgattata aacatcatta ttggtatata
    24181 taatattcca acatttatca caaaatctgt aaatggtatc ataagttgtg aagcacttga
    24241 tgatgatgta tactgataaa aagcaacata aaaagataat gcaacttgaa ggattatttt
    24301 ttgatatgct tttagtccaa gtgatctttt tagctttatt tttataaaat catctaaaaa
    24361 gccaataaaa ccaaaacctg caatacatat aagacctact gccatatcat gactaacttt
    24421 tactcttgta agtccagtta ttagaattgc aacaatcatt ataattccac ccatagtagg
    24481 tgttccgttt ttagctagat gtgtttgggg tccatcatct ctaactgtct gaccaaattt
    24541 aaattttcta agcattggta taaatattgg tcctattata attactatta aaaacgcaat
    24601 caatgcggtg tacgtaagct ctgttattcc taacatcata aaactcctct cccttgtcaa
    24661 ttaactcatt agtttaagct tatttattta gatattcgac tattttttct aatctcatgc
    24721 ctcttgagcc ttttactaaa atagtgtctc ctgtttttac taattcattt aacttgttaa
    24781 acacatcttc tctattctca aagtgatata tattggctga attgaatcct aattgttttg
    24841 cttcttctcc aataaacact gaattttcac ctatagttat tataatatca gtattattca
    24901 tagatgattt tccaactaat ctatgaccat actctgaaat ttcacccatt tctagtatat
    24961 ctccaagtat cgctaccctt ctacctttat atcttccaag tatctttaaa gcagcatcca
    25021 tagaatctgg actagcatta tatgaatcat taattattgt taatttttca ttttttatta
    25081 tgtctagtct catttttgtc cctttaaaat tttttaaacc tttttttata taatctaaag
    25141 aaatatttag acacaaacca actaatatag cagacattgc attatatata ttatgttctc
    25201 ctattgttgg tataaatatt tcctcttttt tcccatctat tacacatata aatgttaaac
    25261 tatcctcttc catagtataa ctttcacaat aaacatcatt gtttttatta aagccaaatg
    25321 tttttaactt atatacatgt tctttttctt ttaatgtaga taaaaactta tcatctccat
    25381 ttacaattaa agtatttgtt tcatcaaagt ttgaagtaat ctccatcttt gctttcaaaa
    25441 taccctcttg tgaacctagt ttttctacat gtgataatcc tatattcgat atgactgcaa
    25501 ttttaggatt tactatatcc actaaatatt ttatttcatt gaatccagac atacccatct
    25561 ctattacagc acactcatgt tctttattta gattaaataa agtcaatgga actccaaaat
    25621 gattatttaa gtttttttca ttttttagta tatttaactt tgcagatatg gcagagtaaa
    25681 tcatatctct agtagtagtt tttcccacac tacctgtaac acctataaat ggaatatcaa
    25741 atttttcttt atagtatttt gaaatatctc ctagagccaa agatgtatct tttacctcta
    25801 ttaaatttat atccgaactt tctaatttta ttccattact ttcatttttt ataaaagttt
    25861 tacatccact atcatatgca gacttcataa atgtatgccc atctaaattt tctccaacta
    25921 tggctacaaa tgcattttgg gcatttgcct ccctgctatc tataactata tcacttacac
    25981 agtcattata atctccatat attaacttcc cattagttgc aagtactaac tcttttattg
    26041 ttaagcactc cattattaaa ctcctccttt aaaaccttag ctatattctc ttagcaacaa
    26101 aaggcaatgt ataccgttat atatacattg cctacgataa ctcttaactt tataattata
    26161 tactatattt ctgttttttt gaagatgttt atgggtatat gttaattatc tgttactttt
    26221 tagttttcaa aatatagggt tatacttgag tcttcattaa cttttactcc aggttttggg
    26281 aatatatcct ttacctttgt atcttctgga agttctatat ctgcatctaa gtctggtttt
    26341 aacttcgcat cctccaatgc ctttacagca tctcctattt ttagatttct tacatctggc
    26401 actttaactt gcttcttttc atactcagct ttttcttctt ctttatatac tggtttaact
    26461 cccaaatatt ttaaagagtc attcattatt tctttcacta ttggtcctgc tgtagtacta
    26521 ccaaatgcac ttactcctgt tggttcgtca acaatagcaa gaactactat ttgcgggtca
    26581 tcacaaggtg ctattccaac aaatgaacat atatactttc ctggagcata cttaccatct
    26641 ataacttttt gagctgtacc tgttttacca ccaagtctat aacctggtat atacgctatc
    26701 tttcctccac cttcagttac aactgattct gcaatttcta acattttttt agatgtttct
    26761 ttagatataa cacttcttac tttttttggt tttactgttt cagttatatt tcctttattg
    26821 tcagtataag attttaccac tcttggttgc attaaatctc caccattagc tattgatgat
    26881 atagctgtta taagttgtat tggagttact gatatagatt gaccaaatga tattgtagcc
    26941 aattcaactg gtcctacatt tttttcgtta tacagtattc ccttagcttc tcctggtaaa
    27001 tctatacctg tcttatccat aagaccaaat gattctatat aatcatacat ttttccaaca
    27061 cctaatctgc ttcctaactc aacaaatact gggttgcaag agttttgtac tgcttgttta
    27121 aattcttgtg ttccatgagg tctgtaatgt ctccaacatt taatctttct tcctccaaca
    27181 gttacactac ctgtacatgt gaatttttct ccatctttaa ttactccttc ttcaagagca
    27241 ctggaagatg taataagctt aaatgtagaa cctggttcat atgtatcact tactgctgga
    27301 tttctccaca tttgataata gccttttatt ttatctttat cattatattt ttcaagttct
    27361 tcttgataat atggatatat tggcgtcctt gagtcattag ggtcataatc tggttttgat
    27421 gctaaagcaa gtatatcacc tgttttaggg ttcatagcta ttgcagtaac cttttttgca
    27481 ttatttaact cataagcttt ctgtactgct ttttcagtat agtgttgaat tacttcatct
    27541 atagacaaca caagaccatt accttgtaca ggctcataat atttttccat tccttgtggt
    27601 atttctcttc ctgaagcatc agtacttact ataagttttc caggctttcc ttttaacttt
    27661 ttatcatatt gcatttctac accagatata cctgttgcat ctgaagaagt atgtcctaac
    27721 acatatggtg caaaattccc atatgggtaa tatctttgat tatcttctgc cacccatatt
    27781 ccacttaatt tagcatctct tatttgactt gccttatcat catctatcca tctttttact
    27841 tttacaagag ccatattttt tttgcttatt aaagcgtaaa tatctttata atcttcatcc
    27901 aaaatctcag ctaccttttc ggctgcttct tttttatctt ctacttccac aggtttacac
    27961 caaacagtat acttagttac acttactgct aactctttca tatttctatc atatatagtt
    28021 cctcttttgg gttctattgg tatatctctt gtttgttgtt ctagtgcttt tgtactcaac
    28081 caatttccct tcataagttg tagataccca gtcctaataa caaggcaaaa aaacaatgca
    28141 catgctagaa taagaacgag taccagcctt ttcttactta tcctctttac ttttctcaaa
    28201 tctattcccc ctagttatcc acatctatat actttatttg tccatctttt ggatagtcca
    28261 tattgagcaa ttccttagcc tttagctcaa tttcttttat tgatgttttt tcagtttctt
    28321 ttgcctttag ttcctctaat actatctctt tttttcttaa atctttttgt aaataataga
    28381 tatcgtactt catttccgag atttttgaat aaccacatag attcaatatt attactgaaa
    28441 aagttgcaat cagtaaaatg catttagctc ctattttttt cttatttctc tttttataat
    28501 tattcttttt tttattttta tattcattta gattatgtat tttataattt tttttcaaat
    28561 tctcaccccg atagtatata gtttaaattt tcctctatac tatatttgtt attaatcata
    28621 tttcttcctc taaaatttca tcgctaaatt aatacactat tacacaatct aattgtattt
    28681 ttttctatac acaacaaaaa cctataaatt cacttatact tttatatgaa tctataggtt
    28741 agatattaat ttctatttat ttttatcact tctagtattt ttagataatt tcttccataa
    28801 atataatatg taactaacta tctactctct gattcctaag ttctccatta tattacttaa
    28861 taccatctat atctatattt ttatatctaa attttcatta tatatctcaa taacaaagaa
    28921 catttcttat agtgttaatt gttttttaat tgctttacca gtgcaacaaa atatataata
    28981 ctttttaatt aattataata atcttatata taagattatt ctttagcctt ataactactt
    29041 atattttttc tgctactctt agcttagcac ttcttgaacg tggatttact tctatttctt
    29101 cctcactagg taatataggt tttctagtta ttattttcac ctcagattct ttatcacatt
    29161 ggcatatagg taaatgctgt ggacatatac aatctgatgc taaatgctta aatgcatttt
    29221 tcactattct atcttctaat gaatggaatg ttattataca tattcttcca ccttcattca
    29281 ttatagaaga agcatcattt atcatctttg taattactcc aagttcatta tttacttcta
    29341 ttcgtattgc ctgaaaagtt cttttagctg ggtgaggtcc atctattctg gctttcttag
    29401 ggatagcttt tttaattaca tctactaatt ctcctgtagt ttctattggt ttgtttgctc
    29461 tttcttcaac tataaattta gctatacgct tagcccagtt atcttctcca taatctttta
    29521 ttatcttagt taattcatct tctgtataag tatttacaac atcatatgca gaaaactcac
    29581 acctaacatc cattctcata tcaagtggtg catcttgcat ataagaaaat cctctatcag
    29641 cttcatcaag ttgatgagat gaaactccta aatcagctaa tacaccatca attttgtaaa
    29701 ctcctatttt ttctaattcc tcttttatat tttcaaaatt gctgtgaaca aacttcactc
    29761 tactttcata ttcactcaat ctctcttttg ctgtgcttat agcattttta tcttggtcaa
    29821 accctataaa taatccctta tctgacagtt tttttactat ttctttagaa tgtcctgctc
    29881 ctcccatagt acagtcaaca tacacgccat ctggctttat atttaaattc tctatacact
    29941 catttaaaag aactgataca tgatgaaatt ccatagttac ctcccttaaa ttcacttttt
    30001 aaactattct tcactaatat tatttttatt tttctttgat aaataaacat gtgcaattat
    30061 gccaccaata actccaatta gacttataac ttgtgccatt ctaagtggtc ctaacattaa
    30121 actatctgtt cttaatccct ctataaaaaa tcttcctatt gaatataaag ttatataagt
    30181 aactataact tgaccttcat attttttatt ttttctaaat agtaataaaa ctataaatat
    30241 tccaaaatcc catatagact catataaaaa agttggatgt acctttactc catcaaccat
    30301 tattccccaa ggtaaattgg tagcacctcc atgtgcttct ccattaataa aatttcccca
    30361 tcttccaata gcttgtgcta aaggcattcc aagtataact gtatcagcca tttttaagaa
    30421 gttaatattt ttaatcttag tgtatatata accagctaat attccaccaa ttaaccctcc
    30481 atgtatagca agtccaccac ctctaaaatt aaaaatttga gacatatttt gagcatagta
    30541 actccaatta aatacaacat aatagattct tgcacagatt agtccaactg gaattgctat
    30601 tatagctata tttaatacat catcttcttt aattcccact ctttttgctt cttttattgc
    30661 tattaaagtt cctaaaatca ttccacatgc cataagtatt ccataccaca tgatgtctat
    30721 gccaaacagt gtaaatgcta ctctatccat aacgtcacct ctttgtatta attgaaaata
    30781 tttccaaagc aatatgctat aattacaatt aattgtaatt atactaaatt atgataacac
    30841 attaatgtta attctacaaa aaatattaaa atttattcta caactcccta cactatatta
    30901 tatctaatta ctataaaatt ttttaaacta aatctctcat gactaaagtt acaagtgtac
    30961 atagatattt tttaatcaaa aagattgatg catatataca tcaacctctt ctaattctta
    31021 tctatagctc ttaaattttt acctcaattt atataaatat attttataaa tttgttttac
    31081 caaaataaac atttaaaaac ataaactaat acttaattag ttattcttat tatgattaag
    31141 tttctattta ggctctacaa tagaaataac acaatactct tctttaaaat gctcttttaa
    31201 tctttcttca acttcctcaa aagtaatatc tttaataata tctaaataat ccaatagatt
    31261 tatatcctta aaaacataag atataaaact attacctata aagtttatag aatcaaagca
    31321 cttaataaat gaccctattt ttttcttttt agttctctca aactcttctt ttgataatcc
    31381 ttcttttttt gatttttcta tataatcaag aattatttct tttacttttt taggctcttt
    31441 agaatctcca gctattatgc taaaagcata atccacttga gacgaaaatc cagctccaaa
    31501 attttcattt attaaacctt gcatatacaa atcttcataa agcttacttc cttttttaaa
    31561 taacatccct acaagtatat cagttacaat ctctttacgt aaaagttctt tcccttttaa
    31621 accaacattg ctatctttaa aacctatgtt gaacataggc atagatatag ggaatttttc
    31681 tataacctct ttttctttta cactttctgg ttcttctgga taaaatcttt ctatactctt
    31741 agagagtctg tctactttgt aattgtttga ctttttagtt acatctataa ctttttctac
    31801 atctaagtca ccaacaacaa ataaagccat atttcctgga ttatagaaag tattataaca
    31861 tttatatagc tcttccttag ttatcttata tatactatca acagtacctg ctatatcaat
    31921 tctagcagga taatttacat acattgcctt caagcaatta aagtatacat tccaatcagg
    31981 gtcatcattg tacattttta tctcttgtgc tattatacct ttttcctttt caacattttc
    32041 atctgtaaaa taaggtgttt gaacatagtc aattagatgt tctaaactct cataaaaatt
    32101 ttcagtagca gaaaataagt aggctgtcat tgtaaaatta gtaaatgcat ttgcatttac
    32161 tcctaatttt gaaaatttat caaatgcatc tcctccatca ggttgctcaa acatcttatg
    32221 ctctagaaaa tgtgcaatac cttcattcac tctaattttc ttatcttctc caattggtac
    32281 aaattctaag tcatttgaac cataattagt agccaaaata gcatactttt tcataaaacc
    32341 ccttttaggc ataaaatata catctaatcc attttgcaat ttctcataat aaacttcttc
    32401 tttcaatata tcattaacta ttttttccat agacatctcc tccattaatt tctcaagaag
    32461 tatacagtat caagctctat attttttata gcttcaacta tatctttttt tgtaaccttt
    32521 tctatactag atattatgtc ttgtacttca gaatttgtct ttgccataga ctgagagaaa
    32581 acaaaatcag acataccacc tatattatca gttatagatt tcatagaatt aactaaagca
    32641 agtttactgt tttctaattc ttcatcagat atatcacctg cttttacact ttctacttgt
    32701 ttttttatca attctacagc tttatcataa tctttagttt ctataccaga tgatataaac
    32761 atgatacttt tatacttctc tattgaagag aatatatagt aacataaact ttctttttct
    32821 cttacattaa caaataattt agagtgtgga ccaccaccta agacattgct cccaactact
    32881 aaagcataat atttatctat gtctgcataa tccacatttg ctctataacc cataactaac
    32941 ttaccttgag ttatttccat ttgttcatct ataactttta cctcatccac attttttata
    33001 aaatctgctc ttggtatatt tataatttct tctctttcaa attttagatt tttacttatt
    33061 atatctacaa ctttatcttc atcaaaatta ccttctacaa ctatatctat aggtgatgtt
    33121 ttaagtatgt ttttataatg ttcatataac tctcttgaag ttatactatc tatttcatct
    33181 tcataaccaa attcacttac actatatctc tctcctttac acatagcctc aatacattta
    33241 tcttgcgcat attccttttt atcatttatt attgattgta tcttttctct taaattctct
    33301 ttttcaatat ttaaatattc ttctttaaac ccaccatcta caacaagcgt attatttatt
    33361 acttcattaa aaaattctac tacttcttca aatatactct catctaaata cttttcactt
    33421 atgttaatta ctttaaaact caatacttga cgttctccac gttttacagc atcagctccc
    33481 atagatgaac catataaatc atctaattta ttggatattg ctctagcact aggatattta
    33541 gcgcttccac ttgctattat tgatggtatt agagcatttt ttgtagtttc tttttcatcc
    33601 aatagtctct gaacatataa acttataaga ttcgatttaa acttttctgt ttttatcaaa
    33661 gtaagattaa tattattacc taaatttatt atttttttag tattaaccat atgtacctcc
    33721 tatttttatt taaacatcaa ttttaaaatt gaaatttatt tattttttaa ttacatatac
    33781 tcacatataa aaaccttcta tttaattata tcctttttat tattttctaa aagcaatatt
    33841 tttatttaaa ttaaatacaa atattattta aatcaatatg ctttaaagtc tagtttacaa
    33901 gcttgatata tatgatgtat aatcaatatg tatgtctata attaaaacaa ttttgcaatc
    33961 ctatagattg attgtttaaa gttatataaa gaatataaaa aagaataaga gagaggacga
    34021 ttaatatgaa attaggtata gttggtttac caaatgtagg taaaagcaca ctatttaacg
    34081 caataactca agcaggtgca gaatctgcaa attacccttt ttgtacaata gacccaaatg
    34141 tgggagttgt atctgttcca gatgaaagat taaacaaact acaagaatta tataattctg
    34201 aaaaaatagt tcctactgct atagaatttt gtgatatagc aggtttagtt agaggagctt
    34261 ctaaaggaga aggtttagga aataaattct tatcacatat aagagaagtt gatgcaatag
    34321 ttcatgttgt tagatgtttt gaggatgaaa atgttgtaca tgtagatggt tctgtagacc
    34381 cacttcgtga tatagaaact ataaacttag aattaatttt ctcagatata gagattcttg
    34441 aaagaagaat taataaaact caaaaagctg ctaaggctga taaaacttta ggttctgaac
    34501 ttgatttatt aaaatcaata atgtctactt tagaagaaag taaatgtgta agaactatgg
    34561 aatttacaga agatgaacaa acattcgtaa attcattgga tttacttaca tctaagcctg
    34621 taatatatgc ttcaaatgta tcagaagatg atttagctga taatggagaa aataataaat
    34681 atgttcaaca agttaaagca tttgcagaaa cagaagatgc cgaagttgtt gtagtatgtg
    34741 cacaaataga agcagaaatt tctgaacttg attctgctga agaaaagaaa gagtttttag
    34801 aaacattagg tcttgaacaa tcagggcttg ataagcttat aaaatcttct tatgctttac
    34861 ttggtttaat ttcattctta actgctggtc caaaagaagt tagagcatgg actataaaag
    34921 ttggaagtaa agctcctcag gcaggaggaa aaatacattc tgatatagaa agaggattta
    34981 tcagagcaga aacaatagcc tttaatgatt tggttgagca tggtactatg gctgctgcta
    35041 aagaaaaagg gttggttaaa ttagaaggta aagaatacat agttaaagat ggagatgtaa
    35101 tattatttag atttaatgtt taaaatgatt aatcgtatac actttgacta ttatatataa
    35161 taactaaaaa actaagatta taataataca atattatctt ataataatta tatagaattt
    35221 aaaaataaaa gaagagtatt tcaaatgatt tgtataaatt att
//
LOCUS       DAYASV010000025        35117 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYASV010000025 DAYASV010000000
VERSION     DAYASV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.025"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            237..1211
                     /locus_tag="ACGI68_001285"
     CDS             237..1211
                     /locus_tag="ACGI68_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFH0137730.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1327..2388)
                     /locus_tag="ACGI68_001286"
     CDS             complement(1327..2388)
                     /locus_tag="ACGI68_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFH0137731.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(3000..3851)
                     /locus_tag="ACGI68_001287"
     CDS             complement(3000..3851)
                     /locus_tag="ACGI68_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137732.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3868..4788)
                     /locus_tag="ACGI68_001288"
     CDS             complement(3868..4788)
                     /locus_tag="ACGI68_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFH0137733.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIKGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4898..5812)
                     /gene="murQ"
                     /locus_tag="ACGI68_001289"
     CDS             complement(4898..5812)
                     /gene="murQ"
                     /locus_tag="ACGI68_001289"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFH0137734.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5911..7008)
                     /locus_tag="ACGI68_001290"
     CDS             complement(5911..7008)
                     /locus_tag="ACGI68_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFH0137735.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLNFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7241..8587)
                     /locus_tag="ACGI68_001291"
     CDS             complement(7241..8587)
                     /locus_tag="ACGI68_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFH0137736.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8676..8984)
                     /locus_tag="ACGI68_001292"
     CDS             complement(8676..8984)
                     /locus_tag="ACGI68_001292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0137737.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9247..9645)
                     /locus_tag="ACGI68_001293"
     CDS             complement(9247..9645)
                     /locus_tag="ACGI68_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137738.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9647..10798)
                     /gene="anmK"
                     /locus_tag="ACGI68_001294"
     CDS             complement(9647..10798)
                     /gene="anmK"
                     /locus_tag="ACGI68_001294"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase AnmK"
                     /protein_id="HFH0137739.1"
                     /translation="MYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEIPKD
                     IKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHGQTI
                     FHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEFLLY
                     SDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYDKNG
                     YFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDLIAT
                     VTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQDEYG
                     YSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11238..12257)
                     /locus_tag="ACGI68_001295"
     CDS             complement(11238..12257)
                     /locus_tag="ACGI68_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HFH0137740.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12251..12931)
                     /locus_tag="ACGI68_001296"
     CDS             complement(12251..12931)
                     /locus_tag="ACGI68_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137741.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GVGYIIQC"
     gene            complement(13083..14990)
                     /locus_tag="ACGI68_001297"
     CDS             complement(13083..14990)
                     /locus_tag="ACGI68_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0137742.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAMFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14991..15743)
                     /locus_tag="ACGI68_001298"
     CDS             complement(14991..15743)
                     /locus_tag="ACGI68_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137743.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16059..17213)
                     /locus_tag="ACGI68_001299"
     CDS             complement(16059..17213)
                     /locus_tag="ACGI68_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0137744.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17216..17683)
                     /locus_tag="ACGI68_001300"
     CDS             complement(17216..17683)
                     /locus_tag="ACGI68_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0137745.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17697..18185)
                     /locus_tag="ACGI68_001301"
     CDS             complement(17697..18185)
                     /locus_tag="ACGI68_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137746.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18199..19530)
                     /locus_tag="ACGI68_001302"
     CDS             complement(18199..19530)
                     /locus_tag="ACGI68_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0137747.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19544..21109)
                     /locus_tag="ACGI68_001303"
     CDS             complement(19544..21109)
                     /locus_tag="ACGI68_001303"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucoside-specific PTS transporter subunit
                     IIBC"
                     /protein_id="HFH0137748.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21279..22064)
                     /locus_tag="ACGI68_001304"
     CDS             complement(21279..22064)
                     /locus_tag="ACGI68_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0137749.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22231..23430)
                     /locus_tag="ACGI68_001305"
     CDS             complement(22231..23430)
                     /locus_tag="ACGI68_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFH0137750.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYINTLEKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTNARNIKLNPVENLPASVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKENKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(23952..25289)
                     /gene="xylA"
                     /locus_tag="ACGI68_001306"
     CDS             complement(23952..25289)
                     /gene="xylA"
                     /locus_tag="ACGI68_001306"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFH0137751.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(25385..26935)
                     /gene="xylB"
                     /locus_tag="ACGI68_001307"
     CDS             complement(25385..26935)
                     /gene="xylB"
                     /locus_tag="ACGI68_001307"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFH0137752.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVSSLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSKVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            27143..28303
                     /locus_tag="ACGI68_001308"
     CDS             27143..28303
                     /locus_tag="ACGI68_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0137753.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(28459..28863)
                     /locus_tag="ACGI68_001309"
     CDS             complement(28459..28863)
                     /locus_tag="ACGI68_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0137754.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNANLLSDDE"
     gene            complement(28884..29366)
                     /locus_tag="ACGI68_001310"
     CDS             complement(28884..29366)
                     /locus_tag="ACGI68_001310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0137755.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(29386..30195)
                     /locus_tag="ACGI68_001311"
     CDS             complement(29386..30195)
                     /locus_tag="ACGI68_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0137756.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(30188..30967)
                     /locus_tag="ACGI68_001312"
     CDS             complement(30188..30967)
                     /locus_tag="ACGI68_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0137757.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(30967..33045)
                     /locus_tag="ACGI68_001313"
     CDS             complement(30967..33045)
                     /locus_tag="ACGI68_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFH0137758.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(33844..34365)
                     /locus_tag="ACGI68_001314"
     CDS             complement(33844..34365)
                     /locus_tag="ACGI68_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3072 family TudS-related putative desulfidase"
                     /protein_id="HFH0137759.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAINLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKIKDIQMVDGKGIFIEELEKLLKEYNLE
                     IPIVGFDELREDK"
     gene            34531..35097
                     /locus_tag="ACGI68_001315"
     CDS             34531..35097
                     /locus_tag="ACGI68_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3073 family putative ECF transporter S
                     component"
                     /protein_id="HFH0137760.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
BASE COUNT        11542 a         6135 c         3769 g        13671 t
ORIGIN      
        1 tttcatttgc tatttaaaat caattttaag ttttcttctt ataataaact tcctctttgt
       61 ggtctcatgt tagtctctaa atttaaaatt ttacaaaaaa ttttcataaa ataaaattat
      121 attgattgta tctaaacaaa tagggcaata aaataattaa gtatagttac ttacctttca
      181 agcaatatta gttttatgtt agaattttat tatattgatt tacaggaggt ttgcatttga
      241 ataatattaa ttcacttaaa gtaataaaat tatctcttgg atgttctctt gctatattta
      301 ttgcttggct attaaaatta gaatattcaa tggttgctgg agttatagta ctacttacag
      361 taaaggacac taaaagagaa actcttaaag gctccattgg aaagatttat ggttttcttt
      421 tatgtactat tttttcatat ttatgtttta atattttagg atataatttg acttcatttt
      481 caatatttat atttataatt ataccacttt gttttttgtt aaatattcaa gatgttattg
      541 ctatgtgtgt agtcatagca tcccattact accttcaagg agaaacttct atcaaatgga
      601 ttttaaatga agctggaata tttgcagtag gaactggaat aggtgtctta ataaatatgt
      661 atattccaac aaatatacat aaaatccatg aagttcaaaa aaagttacag gaagaagtga
      721 gtattgtact tatagacatt gcagatatta ttgtaaatcc taaaaaagaa aatggatata
      781 gtagagattt atatacatta aattcactta ttgacagcag tatttctgaa acttacgata
      841 atattaataa tactttatta agtgatacta gattcttctt agagcatatg gatattataa
      901 aaagtcaacg ggatatctta gaaaatttgt atagttatgt atctcaacta aattctacac
      961 caccacaagc tcatattcta tctgctttta tacataagat aggttatact gaatttgaag
     1021 ctgaaacagg caatcttctt cttgaagaat taaagagact aatgcttagt atgaaaaacc
     1081 agcctctacc tgttgacaga acagagtttg aaaatagagc tattttattc ctatgcttga
     1141 ctgagttaaa acagttttta gttaatcgta aacatgctca gatgcttaga gataataatt
     1201 tttataaata aaaaggggag aaatctagca gttgacctga ctacaaaata tcagatataa
     1261 tattaaatgc gcccctagta agagggcgca atgtttataa cttattctac tgtcatgatt
     1321 aaatcattat tcatatcgaa catacattat ttgaccaagt ccaatattag ctttttcatc
     1381 agtgatttct attttaatag tcacatcatt ttttttacag tacttcgcta attctgcacc
     1441 agaacgattt ttaccactaa aatctaaaga aaatggctta tcatatttaa aaggatattc
     1501 aacaggttta ccctctggtg ctaatgcaaa catcccatat ggtgtgttca taaatcctat
     1561 tacagcaatc gtaagatttg atgggtctaa atttgattca ctacctgtaa atgtaattgt
     1621 atttactgat gaattaacta cactacatcg aacaccttca tacattacat gtttttcatt
     1681 tatatcttct ccttttttcc caaaatttcc aacagccttt gccccttctt gatgtggtaa
     1741 catagtgtgc ttcccaggtg gtgctggttt ctgtgtctgt tctggtttta ccgtctgtac
     1801 tattgcacca acttttggat atgctgtctt atcaaatggt aatgggtggc taagactctt
     1861 gtatatgata tcctttcctg ctgatttatt agcatcaata tctacatgat acaaacgccc
     1921 atcaccaaaa cggataagat tccactggtg gtcatttgat gtgctgtgta cataataaga
     1981 ttcaattccc atagcatcac acaaagcttt catgctccta gccattcctg agcatgcacc
     2041 ttttttagta accaatgtat aggcagtatt tgctccctct actgcattcc agtcaacata
     2101 tgttgtgtta tttttaatgt gattgtaggc aactaaaatc ttagcatctg tggtcatatt
     2161 ctttgtgatt ttcttgttca taaaatctgc tgttgcttgt gctactgagt ttagttgggc
     2221 ttgtgacagc tttggaccat atacgctatt ggttccagtg acatagcttt ttggtgtttt
     2281 tgctgttacc tctttggctg tcataccata aactgtcata ctcatacctg agattaaaac
     2341 tattgttgta atcaataact tggtagctaa cttcctaatt ttcttcatac ttaatattct
     2401 cctttataaa acattgttac ttcctattct ctaccattaa taaaattaat ttaggattta
     2461 aaaaggtgga ttgtcaacat gtatatttct ccattcatac attcgtgaaa tatttaaatt
     2521 tcatggaaaa tctaatagat attttctaga taatttgtat acaatacaac tcgcaaatca
     2581 gggtgtttgc tgttagtttg agaaaatgat gatgattaat acattcggaa tctttgacct
     2641 ataataaata ccattgtctt caatcattaa taatggtaag agtaaagaga attatcaata
     2701 atacagttaa aatcaacttt gtatacattt catacattct ctcccaacaa cattatactt
     2761 cttataattt gtatattcaa tacgttttct gggaaaatat aggatatatc tataaaaata
     2821 taaaaaagga gttgaactta aaatttgatt aaaaaaaatc aattttaaga caactcattt
     2881 gtattataaa aatgctatat attattatta agtaagtcaa tattttagat atttatctat
     2941 ttcttattga cttacgctta tagtctaact tatttgttta gttgatatat aacctagttt
     3001 taagcattat taatatttaa ataatcaagt atcctttttg tttcttgtag tttctgttct
     3061 gcctcattta agttttcttg aattatagaa atatatatta tatctatcaa cgtaagcatt
     3121 gctattcttg aagaaatagc tccttctcta agcctttttt ctatatttgg tactactagt
     3181 tttaaatcac taatcttact aagatgattg tcactatatt tagttataca aatgacctta
     3241 catcctttta attttgcatt ttctatagac tttattactt cttttgattc tccagagtaa
     3301 gatattgcta tagctatatc ttctttatct acatttgaag ataccataag ttgtgtatga
     3361 ctatctaaag aggtaaatgc ctgtttattt atccttaaaa gtttcatctg taaatctagt
     3421 gcaactaaag ccgaaccacc tactccaaat atatatacat ttttagcctt tattatctgt
     3481 tcaactgctt cttttattat atctttttca ttcaacaacc ttgtttggtt gatagtctct
     3541 atgtttttgt ttgtaatttt gtttataaca ctatctatat tacaatcata gttttcatct
     3601 acatatgtgt attcattttc tttgcccatc aaatctttag ctatctcaat ttttaaatcc
     3661 ccaaaacccg aatatcccat ttttctagaa aacctaacta tacttgctgt acttgttttt
     3721 gagtttgacg ccaattctga tacagaaagc ttacttatag cctctgtatt attaagtatg
     3781 taatcagcta tttttttatc tacttccgtt aaatcttcat atatctgttt taattttaaa
     3841 atgcaactca tactctatca cctctaccta tttactataa tatttcttct tagcaagtaa
     3901 acatgctcct aaatctggag tcaacactgg tctcactata ccaaatctcg ttttattcaa
     3961 atatttttta agtggctcta atattaaatc tcctgactta aacactccac ctgaataaga
     4021 tacctttatc tttgtacctt gttcaaaatg atgctctaat cctattataa gcctacttaa
     4081 ttcataagct gcatcatcaa atattttact agcagtatta tcaccttcaa taactgcttt
     4141 taaacatatc gtagcaaact ttgctatttc tattctatct ccttttattt catcatttac
     4201 atattttatt atgtcataat cattctctat attaagatgt ctttttatta aatcatataa
     4261 ttttgttttt gaacaccttc catcagactg cattgtgaac atttttaagg tagccattcc
     4321 tatataatac gctgaacctt catctcctaa tacatagccc catcctccac atctgtgaag
     4381 attcccatct ttatctaaag cctgtgctat tgaaccagta cctgcaatta tatttatacc
     4441 tttttcccca tttaaagaac ctgctaatgc tatttctaca tcattaccta gagtataatt
     4501 tatatgacta tatgcatttt ttgttgctac ttctaatgca tacaatactt cttttatttt
     4561 tccatatcca gcaagtccaa caaatgcata cgttatttct tctacattta tcttagcatc
     4621 tttacaaact tcttccaacc cagttattaa taatttcgtt agattatcaa atcctatttg
     4681 attataatga caagttcctt tggttatggt tcctactata tttaattctt catctactaa
     4741 tgtaaatgtt gttttcgttc ctccaccatc tacacctaaa aaatacatac tatcctccta
     4801 caatatcttt aataattctt tcaatactta atatctaaaa ataaccttcc aatgctgttt
     4861 ttttaatatt gaaaggttat cagtactatt atttatttta caatttttct attgatttta
     4921 aagcttcact tatataacct tcttgtctat ctaggatact ctttgcacta tccttatcta
     4981 aaccactttt tatcataaat atagccaatt taacgctatt atctgcttct tctaagaatt
     5041 tttctgcaac atcatatttt acatttgtag ctatcattat tattcttttt gctctttcaa
     5101 ccaacttttc atttgatgtc ttaacatcta ccatcaagtt tgagtatact tttcccatct
     5161 taaccataga agctgttgtt aacatattac ataccatttt ttgagctgta ccacttttca
     5221 ttctagttga acccattatt acttctgccc ctactataat gtttattggt aaatccgctt
     5281 ttttactcat ttcactattc ggattcatag ttattgatag tactgcacac cccaattcct
     5341 tagcatactc cattgcacct ataacataag gagtccttcc tgatgcagct ataccacata
     5401 taacatcctt agatgtaact cctctatcta ttatatcttg ctttccaagt tctttatcat
     5461 cttcagcacc ttctattgct ttaaatattg cttgatttcc accagctatt atagcttgaa
     5521 caagctcaaa atccactccg taagttggag gacattctgt ggcatctagt attcctatcc
     5581 tcccactggt acctgctcct atatagaata atctacctcc ctgtttcatt ctatctacta
     5641 ttatattaac agcttttgct atactctcct tttgttcttc aacagcatag gctacagttt
     5701 tatcttcgtt atttattaat tctattatgc ctaatgtatc ttctttatct atttgcaatg
     5761 tattcttatt tctaccttct gtaactaaat tttctaaagt ttttattaac atatcatttc
     5821 ctcattttct taatattaaa tttttaaaca cattttaatt ccaactttca taattatatt
     5881 tttataataa attaatattt tcataataaa ttattttgtt gagggtttaa aattaaactt
     5941 ttgccatggt ttaatataat ctaatataaa taattcttct tttcttatat atcctactac
     6001 attcgattta ccactgtttt tcatatcagt caaggctatt tgtaactccc ctgcataatg
     6061 accatattca ctactttcaa ttactatatc tcctcttttt attattttag gagtattaaa
     6121 tagttcaaat ttatatcctt tatatttaac acgagattga gtagacctta tcagactatc
     6181 agatatgtcc cctctattaa aatgtatttc ttcaaagagt atcttcttct caacttctcg
     6241 tatattttcc tctattgcta catcaaatgt aaccatatct tttctcatag aacctaatac
     6301 ctttagctcc tcatatgttg gataacaatt tgatattatt atatcattta tattattcat
     6361 agctattata tgctttactt gaatatctat aggtagattt ctgtgcatct caattgttgg
     6421 aagcccatca gttacaggcc atggtccaaa tgtagtttca ttttgacttg ttacaaaaac
     6481 tgctgttctt aatccatact tattaaatct ttctgtacat tttataaagt gttctaaatt
     6541 taatccacta tatctatgtg gataaaagtt atgacatcct atcaaatttt ctttatttgg
     6601 ttggtagtcc attattgtat ctatataatt agtattatta ctcatattga tttctatttt
     6661 aagattttga ggattaaaag tcattaatgc ctcttcagaa cctgtaaatc ccatatcaag
     6721 ccttattccg tctgccttcg tctttttaaa aaaattcaaa tctttgtagc ttatttctaa
     6781 ttcaccaaat actctgggat ttacatcaag tattacctca aaacctttaa acttagcgta
     6841 ttcatttata cttttaaatt ttttaattat attttcttta gtatctttaa ctgataataa
     6901 acatgaaaat atacgtgtaa atccattttc agcagcttta tctatataat catatatttc
     6961 cttagatgtt gttttttctg gataaattga tattcctaat cttcccatct gtgtcatctc
     7021 cttatatcct atatgtgttt aaatttattt cgaattacac gaaacttttt taaaaatttg
     7081 atgaaaaaaa gtttcatata tatttataaa aaaagaaaag tttacctttt ctttttttat
     7141 aaacaatcaa caaaatatct tcgttactta tttcataact gttatacttt gaaatttata
     7201 ctaatctaat atagttttta atttccaata tagtttctaa ttaataagct tcttgctctt
     7261 caactttttc ttcatgaagc aacctcttat cgtacatctt gacaaatgga tagtaaatta
     7321 ttattgatat agctatacat aataagttaa gaactgaagc tctccaatct ccaccagtgg
     7381 ctaaatatgc ccctactggt cctggtaatg tccaaggagc agtaactact actctggata
     7441 ctaaattagc actcatagca aaccatgaaa ctgttgccat tactattggt gctacaacaa
     7501 atggtattat taatattggg tttaatacta ttggcgctcc aaatattatt ggttcattta
     7561 tattgaatat tgctggtatt acagttgctt ttcctaaaga ctttccataa gctgattttg
     7621 ctctaaatgc aagaagtaaa gctaagccta ttgtagctcc agaacctcca atccatataa
     7681 accattgaaa aaagggttca gcagcaatac ctggaactgc ttgacccgca gcatatgaag
     7741 ctgtattagc atctaataat tgtatccata aaggtcttgc caatgaacct attattgata
     7801 caccatgtat tccaaatgac caaaaaaacg ttattaaaaa tattagtata attactgatg
     7861 gtaatgtgtc agtggctgaa actaatggag ttactaattt agctatgaag ccatgccaat
     7921 caaaccctag gaaataagta acaatagcca ttaaaattat tattaatgcg gctggtgtca
     7981 gagcttcaaa agaccttgtg actgatgctg gaactgcttc tggcatggtt atcttgaatt
     8041 tagatttgtc agtaaatcta taaatctcaa cacctattat agatgttaca atagcaacaa
     8101 acattgaacc tccaccaaga ttagccattg gcatttgcca tccttgagga attgaattat
     8161 accaagctaa aagttctgga ctcttttcaa ctatctttaa taaatcaggg gtaagaccct
     8221 ctattgcagt aggaactatt gtcaaaagga atgtagctgt agctaataaa cctcctgtca
     8281 atccatctaa cttatatgac tttgccaaac tataacctat gccaaatgta gaatatactg
     8341 atataataaa aaatgtcatt ctatatggta gtaatattgt aactgcattt tcctttagat
     8401 actgagttac cccccatgaa tctggtaatg gaggaaatgc taatatcaag aaaaatgacc
     8461 caactattat aagtggtaat gaagctacga ttccatctct tacagctctt aagtgacgtt
     8521 gttctgccaa tctggccata gaagttgata atttagtgtc taaaaattct gtaaatttat
     8581 ctagcatctt ttctctcctt aattaatatt tatttttatt atcattaaaa tttttataaa
     8641 attaactttc ttctaatcta acaacactgc aactactatt tactcaacag ctcttgaact
     8701 tgttttaata actttggtcc tcctaatgga ctataagctt gtgcttgtat taatgcacat
     8761 ggtacactaa cctcatcagc aaaagctttt aaatagtcaa atctatgtct aacttgtgga
     8821 gctaccatag caatatccca tccattttta acttcatcct caaactcttg agtaccaaca
     8881 gccaaaacat ctatatctat tccatttttt gctccttctt tttgtaatgc attaactgct
     8941 atagcacttg acatccctga tgaacaaaca aataatactc tcatattgat tccccctgtt
     9001 tttttatttc ttttcaatca ttatagcatg tatttttgat tacaggaata cgtttgtata
     9061 tatttttttc atattttaca ttttaaattg taattttatt tcatacattt atatcttgct
     9121 ttaattgctt tatttatcta atcattcttt tgtctaaaat tacacactac ttaaaatact
     9181 catatctttt cttaaaaaag aaaaaagact atcttattat taacataaaa tagtctcaat
     9241 acttttttaa atatgtattc ctttatatat ccagaagtaa cctattattg caaatgataa
     9301 agcaaataaa atatacagtc taaaaaatcc caaagaaaaa cctctcttag tttcaacaaa
     9361 gtatcttgta tatgcaactg caaaagatgt aattaatgaa tttcctataa caactccaaa
     9421 tctattatct attccaaata tagataataa caaaggaaaa actacagtac ttactagaga
     9481 tgaaactatt aatattaatc cacttttaaa acttatagtt ggaatattat aatctgttct
     9541 ttgatttttc tccatttgtt tcttagtatt tttattactt tttgtacttt ttgcattttt
     9601 ataattatta tttttctttc ttttcttttt tttattatta gacatgtcat ttacctccaa
     9661 aaggtttagg tgttatattc cctaaaataa ctttattttt agcccctgtt gcagatatta
     9721 cattagaaaa agaattattt aatgtttcat tgcccaagat tacaaatgcc aaagcctcct
     9781 ttgcatcaga agaataccca tattcatctt gagttaatac ttcaacctct aataataact
     9841 cttttatata tcctattaaa gttagattat gagctcctcc acctccaatt aataaagtat
     9901 ctacattaac ctttggaagt atgaagtttt tataattata atatatagct tttgccgtaa
     9961 acatagttac tgttgctatc aaatcatatt tatctacatg tttatatttg ttaagcatgt
    10021 tatccaaata aacttgtcca aatacctctc ttccagtgct tttaggtggc gcttgactaa
    10081 gatacttatg actcatcaaa tcttttaaca tttcttcatt aattttacct tttgatgcaa
    10141 agtatccatt tttatcatac tttctattaa ataatctttg acatactcca tctattatca
    10201 tatttcctgg tcctgtatca aatgcaaata catcatctat gttacatgat ttaggtatta
    10261 tagtaatatt tcctattcca ccaatatttt gaagtgctaa atttttattg ttatcactat
    10321 ataataaaaa ttcactatat ggtactaatg gagcaccttc tccacctgct gctatatcca
    10381 ttactctaaa attagatatt acctttgtat ttgtttcata tgctataact gctggttcac
    10441 ctatttgaag cgttgaagat acaaaattat tataacttct tggtatatga aagattgttt
    10501 gaccatgaga tgcgataaaa tccaaatttg ctatatgaaa gtttgcttta tgacacactg
    10561 attttacagc tttactaaat aaatatccaa gcttaaaatt taagctacag attaaatcaa
    10621 cactcgattc ctcttctata cagcactttc ttatttcgtc ttttatatct ttaggaattt
    10681 ctagagttat aaattctatt tgcttaactt tagtatttct accattccca ctaatttcag
    10741 ctagtactgc atcaactcca tctaaggaag ttcctgacat taaacctaca ctatacatag
    10801 ttatcatcct tttaagttta tagttattcc tccccaaatc aaggtattaa gtttaataat
    10861 tttagaatta atcttatttc atctttaatt acgataattt actcttatga aattaattga
    10921 gttaatctta tacacgtttt ttaccttagc taaatcttac attaatttat tttcagtgtc
    10981 aatgtaatac catgtaattt tttttcacaa acaaaacata ttctctctcc aaaaaccata
    11041 ttaaaatagt tactacaaat aaaaaacata ccaggtacct atctgatatg tttttcatat
    11101 ataataatta ttattttata atatagatag agcatcattt aaggcactaa ttaaatcttc
    11161 actattttca agaactaaat ttattcttat tgtatatgtc gatttgtata tgatgataga
    11221 ttttatagtc taataaccta actatctagg cttaaataat ccgatatatc ataaaaatag
    11281 atattaaaaa ttgtatatga accattttcc gaacatacct caatctcatg ccctaaacta
    11341 tctatcattt tctttacaaa atacatcccc attccagtag acttatacaa ttgatttctt
    11401 ccatttttcc ccgtaaatcc cttgtcaaaa accctagata tatcttcctc atttatacct
    11461 ataccaaaat cttttataga taataccact ccattttcta acttttttga attaaattca
    11521 atcttaccaa cttcctttga atactttata gcattattaa taatttggtc taaaacatat
    11581 gacatgcatt tagaatctgt gtaaacatca ttttctaatt cattcaaact aacttctaca
    11641 ttatttttta taagcaaaaa tgaattattt tttatagaca tttttatcaa tttttctaaa
    11701 ttaaatttat ttataaatac atcttcaaac atagtgttgc ttctactact atacaaaata
    11761 gagtttacta aaaaatttat ctttgcaacc tgattcttaa tatccaaact atcttctatg
    11821 ctacttaacc tatctgtaat tatacttaaa gaagatattg gaatttttat ttcatgaacc
    11881 catttagcta tgtactcatc caattctttt aatgattgtt tataagtctc tatgtcacta
    11941 ttatttttta tttttaagtt ttctataata tcttttatta gattttcttc aaaagattgt
    12001 ccatcaacta aataactgtc tatttcacca ctattttcta tacaattata taaatttgtg
    12061 taactatttc taaaatttat ataatctata acaaaaaata ccattattat aaacagtact
    12121 aaaaaatcta aatatattaa ctctgaatat ttatttttaa aaatatctat agcaaataag
    12181 tagatattta ttatactaac tacaactaaa ggtagagcaa tatacttaga cctacctttc
    12241 aaatatttta ctagcattgt attatatatc ctaccccctt cttagttttt ataaaatcat
    12301 caaaacctag ttctttaact ttacttctca atctattcat atttacagta agtgcattgt
    12361 ctgtcacaaa ctcatcatta tcccatagag tcatcataag actttccctt gatacaaccc
    12421 tattcttatt ttttaataat aatgttaaaa tttttatttc gtttttagtc aactctatac
    12481 ttttatcctt atacttatgt tttataatcc ctttccccac atcaaacatc aaatcttcac
    12541 aatacaaaat actattagaa tcactataat tatagactct tcttattata ccatttattt
    12601 tggtaaccaa tatttctata gaaaatggtt tggtaatgta atcatctgct ccattattta
    12661 tacccattat taaatccata tcagaatctc ttgatgataa aaatattaat ggtacatttg
    12721 aaatagctct tattttttcg caccagtaaa atccattata taatggtaaa tttacatcta
    12781 aaagtactac atctggtttt ttatctataa attcatctaa tactacctca aaatcatcaa
    12841 ttaattctac tttaaatccc catttttcaa gtgctaatgc aatctctttt gataaatcaa
    12901 tatcgtcctc tactaataaa attctataca tttgtatatt ctccattatc ctaatatttc
    12961 actatgctat gaattattaa tatcataaca tattttatat gaatttatta cccaaattat
    13021 ttagttaatc aacaaggaga ctcttattac aagaatctcc tttctcttta ttaatatatc
    13081 aactaatttt tatacactac actcttaaac ttattaacag atactgctcc atatattatg
    13141 aatattatca aacatatcac taaacttagt atatatatac caaataaatc ggtcatcaat
    13201 aaatctcctg ctaatctcaa tgcaaaataa ctagatatag ttgcacttaa cattggcaaa
    13261 gtataaaaca tagctacttc tttaagtata gctctattta tatctttttc tgtagtacct
    13321 atttttaaca aaatcttata tttctcttta tcttcatatg catcactata tattttcata
    13381 tatattatac ttccagctac aattgcaaag actaagaata aaaacacaag cactacatat
    13441 gcaaacttca tccattgact ctcaccttga gctttatatc cattttgaat atacccattt
    13501 tttaaactta aattcttttc aatattgttg aacattactt gactattttc ttcttgaaat
    13561 acctttgcac cataaaaata cacttgttga cccttatctc ttagtttaaa atatgtatca
    13621 tcatttacca ctataatact ttgattaagt cctgtcccta gtactggaac ttttacttct
    13681 ccccttgaaa tattatataa ctgaccatca agttttatct tggcttcttt tgtagctatt
    13741 gatgctatcg tttgagggct ttttaactct attacattat ttccactaac taatctttca
    13801 ccatactctt ttaattcttt atcattgcca ttcactttaa gagtattaca aaaatcacta
    13861 tatttcatta tagtatctgt tgtatcataa gcttctttta tatctgtttt ctttaaaatt
    13921 gttagttctg gatttactga gtactttatc ttattagtat cttttattat attctctatt
    13981 tgttttttat tcacttcatc tttatccatt gaaactatgc taatagtgta cacatttctt
    14041 tgttcttggg cattcttatg tatacgcttc atcgcaactg aagcaccaag agttgatatt
    14101 gttgtagtta taattatagc tattatagca taagttttat agtttttatt taatcggtag
    14161 tttatattgt ttattgcaat tatattattt ccattaaata atattttttt atttttgatt
    14221 ataatatcaa atataacagg cataactgat ttgaataatc cataagtacc tactattatt
    14281 atacctataa caggtagtac actagttata tctccagttc ttatattggt agaaagatag
    14341 tagccatatc ctattactat aattgatact atagctaata tatatatacc tattcctacc
    14401 tttggtattt tttcttgttt tttagatata ttgattagat ttattacact acttctacat
    14461 attgttataa aaccttttac agccattatt ataaatatag aaataaagat taaagtagca
    14521 tatagtatag cttctatact aagtccatta gataccttta tattaaatcc agataacttt
    14581 attatcacta cttgaaaaaa tctagatgtt attatcccta taattattcc tgttatacaa
    14641 gaacttattc ctataagcat attttctaca aaatatattt ttcctatagt atataaatct
    14701 aatcccataa aagacaatat tccaatttct ttagacctat tcttaaaaaa tatatttgtt
    14761 gcataccata taaagaaaaa taaaaatact gatagtatca ttattacagc tactaacact
    14821 gatagtgtaa attttttatt ttcatcttgt aacacattta tagcatcacc atatcttaat
    14881 aattcaaaat tagttagcac aactactgaa aaaaccatag atataaaaaa tgctagataa
    14941 gttcttatgt tactcttaaa attattataa gcaattccaa gtatactcac ttacatctca
    15001 cctcccatag aggctagcat atccataatt tttccataaa attcttttct actatttcct
    15061 ttgtttaatt tacactttat atttccatca tttatcatat atacttcatt tgaataactt
    15121 gctgttagag ggtcatgtgt aatcattata atagtagcgt tattttctct gtttattttt
    15181 tctaatgatt ctaaaagttc atatgctgat ttagagtcta aagcaccagt tggctcatca
    15241 gcaaatatga ctgttggatt tgtaattaat gacctagctg ctgcgcctct ttgtttttgt
    15301 cctcctgata actgatatgg atatttatct aaatgatttt ctaatccaaa ttttttagct
    15361 atagaaaata ccttagcttc tatctcctta cttttctttt ttcctaatgc tagaggaagt
    15421 gctatattgt ccattagttt catattgtct aatagattat agtcttgaaa tataaaccct
    15481 attttttctc ttcttaattt tgatagttca ttatttttta cttttgatat attaattcca
    15541 tctagtatta tttctcctga agtttgtttg tcaagtgtag acattaaatt tagcaatgtt
    15601 gtctttcctg caccagatgg acccattatt gatataaaat caccttcatt gactgtaaga
    15661 gatatatttt ttaacacatt tgttttaaat ccttttattc cataatcttt tgatatattt
    15721 tttattttta atatttcttt cataataata gcctccagtt gctaattttt taatatatat
    15781 ttattttaaa ggttttatat aaataaatcc ttaagtaaat attacatttt aactgttagc
    15841 attacacttt tgtaacatat ggaagactat tataattata gtagaaatat cttaaaataa
    15901 aagcatgatt tacttctttg tataaacaaa gaaaagaagc tcctaagaag cctctaatct
    15961 atacaattat tcttctctat aaaaaaccaa aatacacatt gattgtggca aatacagata
    16021 ctataaacaa gtgatattac tactatatat taaaacaatt attcctgaag ataattcaat
    16081 aagactatca agcaataagc taagcatcca aaaagactta ataccattaa tgtataatta
    16141 tacccaatat taatgctcaa aaaaccagca acagttggaa taatcagaga tgaacctccc
    16201 ataaatatac ttaaagctat taattggcta ctacttctaa gttttttagg agaaacttca
    16261 aatattaatc tttttgaagt aattaaaatt aaaggagtag aaaacacttg aaaaacacac
    16321 agtataataa tgatatttga atcatttgtt aaagcaaata atagaaactg aactgttatc
    16381 ataatagcac ttaattttaa taagtttaaa ctgctatatt tttttaataa ataagaacca
    16441 gccagatatg ttggtatctc taacaatgac tgtaaagacc actttaaaga tatctgagaa
    16501 tttgtagcat ttaaagctat cattttatct attacagtac agttatttgc taccaccatt
    16561 gagtataata agaataaaat acatactagt aagatatatt ttttatcttt taataattca
    16621 ataatatctt tattagttac atttattttc ttattaatct tttcaatatc aggtaataat
    16681 agtatattta taataagtaa tactacaatt aaaccaatcg ctacagataa tccaaaatat
    16741 gagaatctaa gaatcaagta agatgctacc atacttccaa tagcccatcc aatcgaccca
    16801 aaagtcttaa taaatgacaa ataattatta atattatctc cacaacctat tatccatgta
    16861 tcatataaac tacagcaagt atttaataat cctcctgata aagcaacaag taataagtat
    16921 attataaaag ttgcagattc agtagaaaac ataataactg atgcaattcc ataaactgat
    16981 aaactaatta aaacaatttt tttcattgtt tgatacttat cagctaatac tccaaataat
    17041 aattgaaaca atatattggt tattgcatag ctcgataata taattcctct ttcaaacgct
    17101 gagtaaccta attttgataa gaaaggtgtg aattttgttg aagcaaaggc aaaaataata
    17161 tatgagaaaa aatagaatat gataaattta aatttaatcg ctttattaat cataatcaat
    17221 caaaattgat aactttaata ccactatttc cttcctgatt taagtttgaa agatgatatt
    17281 ttaccgtatt aggattctct acaataacta aaggaatata taaagtattt tcaagtaact
    17341 tattatcaat ttctactaac ttttccccca tattaacttc ttgaccttgt ttaacttcta
    17401 atttaaagtt ttcttttttt gttgttgcac tatccaatcc aatatgaata agaatttgaa
    17461 catcattttg caatttaatt cctatagcat ggttagtttg agcaataaaa cttattactc
    17521 ctttagtcgg tgatttaaga atattgtcct ttgctatcat agcaaatcca tctcccatta
    17581 ccttagttga aaatacttta tcaggaattt ctccaagatt aattattttt ccatcaatgt
    17641 atgaattaat tgtaattgct tctttttttc ttttaaagaa cattatagac actcctctac
    17701 ataattaact ttattctaat tatcattaca aataacgata ttaagcatac tgcaattgag
    17761 gaaaaaagag ccttatagct ctttccccat tgtagttctt taatactgct tatgatttgt
    17821 ttaattccaa caaatttata aatataggta aacatgataa caatagttat tgtgtaaatt
    17881 tggaatatct gccctaataa gactaaatag aaagcaatca tgttttcttt tatctcttta
    17941 ttctttttta tattgtaaat aacatatgca ctgtatactg cactaaagtt agagattaaa
    18001 tttaaacttg cattaacatc attgtttaaa agttgtgaaa atgaaatatt tgtaaataat
    18061 gatgtcaatt ctaaatacac tatatataaa acaggtaata ttagaaaaca aaccataacc
    18121 actgaaagaa atatattttc atcttttaat actgctgtga tattttttat tttatttgtt
    18181 atcatgtttt tgttctcctt atttaaaagt tggccagaaa tccttattag ccacaatcat
    18241 ttcatctaag atttcttttg ctacttttgc acttggaaca gttttactca tagtaaacga
    18301 ctgccataat ttttgataac ttttttctat ccatgcttct acagctaatt tttcagaagc
    18361 aacttgttgt tccattaaac ctttttggaa agttggaatt ttacctatag acataggttc
    18421 aaccatatct tttccaacaa ggcaaggaat ttcaaccatt gcatcatctg caaaattttc
    18481 aatagctcca ttattaggaa caatcagtaa aaatctttcc tgagtattaa atgctaatgc
    18541 acaagctaat tcaacaataa attgagaatg ttcacttgca tcaaaccatg tatctcttgc
    18601 tgtgccattt tttataattc tttcacattc ttcgaatgta tccttttctc ttctatccat
    18661 aatttcattt gctcttgtat aatttggatt agcatgttca acagtttcat cagccattaa
    18721 ataatatttt aaataagtat taggtaatgt agtcggttct atttccaata aatcctttgc
    18781 tgctctcata gtttctaacc aactagattc tttgtgttga ccttgaggtg taaattcctc
    18841 atatcctttt ttagcacaat attcctgtaa tttaggcatt aaatcatttc cttctttgtc
    18901 cttaatactt gtccaccacc cgaaatgatt caatccataa tatctaacat caaagtcttt
    18961 acgagaattt aaacctaaaa ctctagcaaa tatttcctct aaacatactg gcatatcaca
    19021 aatattaata attcttgaat ttggtcttaa tacacgacat gcttctgcaa ctatagctgc
    19081 tggatttgaa taattaagca tccaagcatt tggagaatat ttttccatat aatcaatcat
    19141 ttcgattatt ggacctattg ttcttaatcc ataggcaatt ccacctgccc cacaagtttc
    19201 ttgaccaaca accccatatt ttagtggaat tttttcatct tgttcacgta aagcatataa
    19261 accaacacga atttgtgcca ttacaaaatc aatatcagta aatgcttctt ctggatttgt
    19321 tgtataacta aatttaattt ctggattttt ctctctaaca attattgcac atgcttcagc
    19381 aacttttctt tgtctctcat cattattatc atataattta attgattcaa taggaaattt
    19441 agcaacactt tcaattaaca ttgttacaat tccagcagta taagtactac ccccaccagc
    19501 aattagtatc ctctgtcttt ttctttccat ctttccttct cctttataat aaattttcaa
    19561 attcttctct tacacttggt actgaaagcc caataataat ctgaatagct tttccattat
    19621 gcactaaacc atgtgcacct actgccttaa aatcttcttc tttagaaata attgagtcat
    19681 ctttcaaggt taatctcaac ctcgtagcac agtttgtaac atctacaaca ttttctgctc
    19741 caccaactaa ttttatgtat tcagatgctt gttgagaggc tttactcaat ttatttcctt
    19801 tttcatcttt agtttctaag tattcatttt tagtatatag ttttgtttct tcactatctt
    19861 cctctctacc aggagttttc atatcaaact tcttaattaa gaaagtaaat acaaagaacc
    19921 agataaatga aaatataatc cctacaacaa cttgaacaac atataccttc cagtgataca
    19981 ttccaagagg aagccaattt aatacaaccc agttaataat tcctccacca aaatcaccaa
    20041 caactcctag ggcaaacgaa atcgtttgca agcatgcatc taaaaatgca tgtactagga
    20101 ataaaagtgg tgctacaaat aaaaatgtaa attctaacgg ctcagtaatt ccagttaata
    20161 ctgcagtaag agtagcagga atcattaaac ctaatacttt tttctttttt tctggctttg
    20221 cagttacata gaaagcacca aatacaccta atgaaccaaa caccttcgat aatccggata
    20281 atgaaaatcc gattggatac atttccttta gagtcttagc tgaagtttga aaatcctgaa
    20341 tatgagtagc ccaatatact gatgtaccac ctggaacaac aacactatca taacaaattg
    20401 gtgcatatac aaaatgatgt aatccagttg gtattaaaat cttttgtaag aatgcataac
    20461 accaaactcc tatatttcca ctatttataa aaaacgtttg caaatgtcta ataacatttt
    20521 gaatatgagg ccaaataaaa caagttaata atgcaactgg tatcataaca aagaaacaaa
    20581 ttgcaactac aagtgatgag cctctaaata atccaataaa atctggcagt tctttatcat
    20641 aaaatctatt atgaatataa atgacaatac ctgatattaa caaagcacca accataccag
    20701 tatctaatgt tttaatacca gcaatgctta caagtccact tccagcactt atttcagctg
    20761 cgaaatcaac tccaaagctt tttccccaaa aacttaacat agaacctaca aaataattaa
    20821 aagtcaaata cgttgctaaa gcctccatac aagctcttgc ttgttgcttt tttgcaagtg
    20881 ctattggtaa agaaatagca aataataatg gcaattgatt aaataccatc caagctccac
    20941 tagatattac accccaaaat tggtaccaaa tattttctgg tgtagcaagt gaacccataa
    21001 ttgactgatt ttgaaataca attgagaatc caaccattat cccagcaaaa gcaaataata
    21061 gtgttggtgt aaacattgcc cctccaaaac gctgtatttt cttcatcata taaattactc
    21121 ccctcctcat atttttagtt tatctttggc cttagatatc tattatttta acaggttaat
    21181 aaatatatac aatctttagg gtacattcgg agcttattca cagtcaaata agtttttgca
    21241 catttttatg ttcaaattct cataaaaaaa aagaatgact acaatttgca atcaatccct
    21301 tctgtaatca ttctatgcct tttataaata ctatatttaa taaataataa ctcaatcaat
    21361 ataaaatata acattgtcaa tttataacta gggtgcattt gtaaaaatga gatattagtc
    21421 gctgaaatat aaattgattc atcacttaaa tcagtcaatg tattattttt aaactctgta
    21481 attgaaatta ttttagttcc tattaatttt aatcttctag caatattaag tgtattctca
    21541 ttctcaccac ttagggatat aataatcata acatcatttt tactgacaat attgtaaaaa
    21601 gaatcatcaa atgttcttcc ctcaaaatca tatattattt cttgacatga taagaacatt
    21661 ctctttatct gttttgcaac cacattttga atatcacctg aaccataagc gtatattgtt
    21721 cttgattcat aaagaagttt acacaatgca tcataatctt tatttttttg atcttcaaca
    21781 tacttaatta ttgaattaca tgcaatatca aatgactgat tttctatgac atgaaccttt
    21841 tctgattccc agcttaataa tactttaaac tcactgaaac cttttaaatc taatttttta
    21901 acaaatcttg taatagttgc acgagacaca ttacatttca tagcaagttc atttattgac
    21961 atcttactac aatgtttttt atttgcagtt atatatttcc atattaataa atctgtatca
    22021 ttcaatttat tataatttaa aaaaatcatt tcttctaatc tcataaacta ttaccccctt
    22081 taattaaaaa tcgtcataaa tattatacca atattcaaca ttaaattaca gcgtattctt
    22141 atatttacta tttagcccat aaaattgact ataaaaaata ctgctataat tgatgtttaa
    22201 aaaaatgtag ttttaatttt attcttttat ctaataatta atattttcaa gttctagtgc
    22261 ttttcttata caattatcat ctttaaaatc agcagtcctt ttacaatctt ttactttaaa
    22321 ttctggaata gttttaaatt cctcatcaca tattccattc ttatttaaat acatacaact
    22381 tgccattcta actattagac cactattttt taaattaaac aatacagggt ctatacaccc
    22441 tccatctccg ccagtagttt caccaactaa agtcgcaaat ttattttctt tacaaaacat
    22501 tgcaaatgac tctgatgatg aatatatttt tctatcaaca agtaagtata tattaccctt
    22561 aaattttgaa tcatctgtag attttatagt ataggaagta tcttcaaaat cattaaaatc
    22621 ttttataatt tctttaggtg cattttcctt tacacttgct ggtaaatttt ctacaggatt
    22681 taattttata tttcttgcat ttgtatattt ttttactatt tcattattat ttctataaat
    22741 tctataacca tttattttga catcattttt tatgagttta gatacgattc cctgccaata
    22801 cctatcactt ccacctaaat tacctcttat atcaataatt aaagctttat gtttttctaa
    22861 agtgttaata taatctccaa ttatttttaa atcattattt atagagccat tctttgatga
    22921 cattgatggt aaatacatat atccaatctg acccttaata acatccttta aaattaattc
    22981 tttttttaca aaaatatcat ctggcatttt tattttaaga ttcatagagt catatctatc
    23041 cacaactttt ttatcattaa gaaaatcata ccaatcatta ttagagtatg atttcttaaa
    23101 aagttcatat cttttcttat tatctattac ttctgtgtgt ttattattta aatcagataa
    23161 tatagatgac agttcttcta taaattcttc atcattttca gtgtttttta ttcgttttgt
    23221 atattcatcc ttatttgcta accaatcaat attattaagc ctcttattta cacctagata
    23281 tggatatcct gtctttacaa cattatacac ataattaaag tcatccacct tttcctcttc
    23341 agacaactga ttcttttgag aataagtaga attacctaat aatattataa atgctaaaat
    23401 aattactaaa aataatactt tctttttcat gtaattctca tccactttca gattttatat
    23461 tttaatacat tgtatcatta ttttattatc acctaaacag ttaaaagaaa ttcttgaaga
    23521 tatattccac tattattctc tttaaaaatt tttatttgta ttttcaaaat tatttcatga
    23581 aagttataaa ataacaatta tattcaaata tctattttac tgctatatcc tgaaagtaaa
    23641 ttcttatcag tgatatacta aaaaagatat gctaaaaaat ttatccatta taatgactta
    23701 aatttagata ctttactgat ttttatttat tagttgagaa agatattgat aattaaaaat
    23761 attgataact atagaaatga ttatttttta tagaaatttt aaggatataa actaattata
    23821 cttaagtcta tatccttaat ttatcatgat tatgagaatt tttacaaata cttatctttg
    23881 tccgttttag aataaaaaca agaacatatc ctgttatttt attcaaacat ccaagtttaa
    23941 ttactacaca attacttatt agaaattgta tctacttcat atatatatct gttaagtata
    24001 gcttctagca tttcttgtct tccagactta ttctttataa ctggcatttg cagtgcataa
    24061 gattctaact ctttaaatcc aaccttacct tctactatat ccttacctat accttcacta
    24121 aaacttgcat atctttcttt agtaaagttt tcaaatacct catcttctaa taatttatga
    24181 gctattaata atccctttgc aaatgtatcc attcctgcaa tataagctag gaataagtct
    24241 tctacctcaa aagatgctct tcttacttta gagtcaaaat taaaccctcc tggtgctatt
    24301 ccaccttgct ttaacacttc atacatagca agtgttgcat catatatatt tgttggaaat
    24361 tggtctgtat cccaacctaa taataggtct ccttgatttg catctatact acctaaaaca
    24421 ttgtttattc ttgctatatt taattcatgt tggaaagtat gtcctgcaag tgttgcatgg
    24481 tttgcttcta tattcatttt gaagtattta tccagattat actttcttaa aaatcctaaa
    24541 acagtagcag tatcaaaatc gtattgatgt ttagtaggct cttttggctt aggctctatt
    24601 aagaattgtc cagtgaatcc tatttcctta gcatagtcta cagccatttg taagaatctt
    24661 gcaaagttat ccatttcaag ttctgtatta gtgttaagta aagtttcata accttctctt
    24721 ccaccccaga atacaaaatt ttctcctcct aagtacttag ttatttccat agctttttta
    24781 acctgagctg ctgcatatgc atatacatct gcattacatg ttgtagatgc accatgaaca
    24841 aatcttggat ttccaaacat attggcagtt ccccataata actttatacc tgtcttttcc
    24901 atattaactt ttatatactc aactatttca tctaatatct tatttgtctc ttctaaatct
    24961 cttccttctg gtgctatatc tctgtcatga aaacagaaat actcaattcc catcttagac
    25021 ataaattcaa atcctgcatc aactttaatc tttgctatct caattgggtc tgttatatta
    25081 ttccaaggtc tctctactgt gcctactcca aatgggtcat taccctctcc acataaagta
    25141 tgccaatagc tcattgcaaa tcttaagtgt tctttcatag tcttattacc taccacttgt
    25201 tcaggattgt agtatctaaa tgctaattca ttgtcagatt ttactccttc aaattttatc
    25261 tgtcctattc ctttaaatat ttcactcatt tcatgtcctc ctaatatttt acacatataa
    25321 tatatgtagt atgttttctt aatttaaaac tattttctat ctttattttt ctttttaagc
    25381 taatttaatt atttattttt gcattaacta cttcatctaa taacttaaag tcatctttca
    25441 aagatttgta tattctttta tacaatctat gatatatatt gtatgtatct acattttcac
    25501 ttattggttc taatttagtt gaagttttta ttatagcatc acaagctttt tgtatatttt
    25561 catatattcc agctcctact aatgcaagaa ttgcaactcc aagtgctggt ccttcacttg
    25621 aatttatagt atatacatct tgattaaaca tatctgcttg catctgtctc catactttac
    25681 tttttccacc tccacctgag gctcttactt cattaacgtc tatattaaga ttctctatta
    25741 tatccataca gtctttaaga ctatatgaaa caccttccat tattgacctt gcaatttctt
    25801 ttttagaatg gattggagat aatccaaaga acacacctct tgcataaggg tcaagatgag
    25861 gagttctttc tcccatcata tatggtaagt aaagcaatcc attacatcct gttggtactt
    25921 tacttacttg attatttata atctcatata catcttcacc taaagaattt gaaacttcca
    25981 cttcactttg acagaaatta tctttaaacc attttaatga caagccagct ccttgagtaa
    26041 ctcccataac atgccatgta tttggtattg catgacagaa agtatgaact ctaccttctt
    26101 tatcaatttt tggctctttt gtataagcaa atactacacc tgatgtacct atagtagatg
    26161 aaactactcc atctttaact attccatttc ctatagctcc tgctgcttgg tctccagctc
    26221 ctcctaccac tattgttcct tctttaagcc ctgtcaaact tgatacatct ctagtcacat
    26281 aacctgtaat ctcttgtgat tcataaactt tacctaaaag atttttatct atatttaact
    26341 tttctagtac ttcatcgctc caatctcttt ttcttatatc gagcaactgc ataccacttg
    26401 catctgatac ctctgtagca tattcacctg tcaatttgta tcttatataa tcttttggaa
    26461 gaagtatttt attagtattt ttatatatat ctggttcatt gtttctaacc cataatatct
    26521 ttgatgctgt aaatcctgta agtgctggat tagcagttat ttcaatcagt ctttcttcac
    26581 ctactaaatc agttatctcc tcacactctt ttactgttcg ttggtcacac catattatag
    26641 agtttctcaa tgttttacct tcattgtcca gcataactag accatgcatc tgccctgata
    26701 atccaattcc ttttatacat tcactgctta tatttgaact ttgaactact tctcttatag
    26761 attcgactac agctttccac caatcatttg catcctgctc tgcccaacct gatttctctt
    26821 gtataagagg atattcatat gtacaacttt ttatagtatt tcctagttta tcaaataaaa
    26881 ctgttttcgt acctgatgta ccaatatcca cacctaatac atactccaac ttcatccctc
    26941 cttgaatgcc acttcataac tatcgttttc ctatcctaat ttttttatat tttaggtata
    27001 ttttacttgc tttatttttg ttagtatatt aaactaactt atgaactaat aataaaatat
    27061 ttaattatct ttgtcaatgc aaaataacct tttatttttg tgtaaaaagt attacaatat
    27121 tattttaagg aggaatatca aattggttac tgataaatac actattcgag aaatgaatga
    27181 aagattggta cttgaacaaa ttataaaaaa tggtcctatt tctagggcaa gtatagcttc
    27241 tactatagga ttgaataaag caactatatc cgctattacc aaaaaactta ttgatgaatc
    27301 tctagttcat gaaataggaa ttggaaatag tactcatagt ggcggaagaa aacccatatt
    27361 attagtattt aataaatgtg cagggatttc tttaagtatg gacattggat acgactatat
    27421 attttcttct ctatcttact tagatggaac tattataaat tctaagaaat taacagatat
    27481 acaagtaagt aaagacaatg ttatacaatt gatagatgaa attataaata gttataacat
    27541 atccaaaata gacactcctt ataaggtaat tggtcttact ttggctatac atgggattac
    27601 ttgtgaaaat aaagtattgt ttactccata ttacaatctt aatgagattg acttatatag
    27661 catccttagc aaaaagtatg actttcctat tcatatagaa aatgaagcaa acttaacagc
    27721 tttagctgag aatacttttt caactgtaca taactcacta cttagtctca gtattcacag
    27781 tggatttggt tcaggcatta taataaataa taaattatat agtggcagaa atggtatgag
    27841 tggagaaatt ggtcacacta tcatcatgcc taacggtaaa ctatgtcctt gtggaaatcg
    27901 tggctgttta gaacagtatt gttcagaaaa aaaggtattt gaacagcttt cttctttaga
    27961 aaatattcct aaaatagatt ctgatatagt aaagcaacta tattatgaag ataaccataa
    28021 tgccaaaaaa gttattcatg agttttgtag ttacttaaca atagctataa ataatgctat
    28081 tacaacttat gctcctgaaa taatatattt aaatagccaa ataattagtg atatccctga
    28141 gatattacag attacaaaag atatgttagt tagctctttt aacaaaggaa taaatataga
    28201 aatttcatca ttagggtctg aggcatctct atatggtggc tctgctgtaa atatcaagag
    28261 ctttttgaat atacaaaatt taactttaat taatgaaata taagggttat gactatatac
    28321 agccataacc ctttaaatta tacattctaa aattagattt attatatagt ttatgcaaga
    28381 ttagctatat tttttaaaaa ttgtatatca attaagtatt aacataagta tttcgtttta
    28441 aatattaata taatcttcct actcatcatc agataataaa tttgcatttt gttcaaccat
    28501 agaaatatta tctttaccta ttttctctaa ttttgctgcc aattcctttg tatctaaaaa
    28561 agctgattca ataaccatac ctaaatttag ccctgaaata attttaattc gactgtcatt
    28621 attggctact aataaagcag cattgcatgg agttccacct agtaaatctg ccattataat
    28681 tatttctgaa ctttccttgt acacttctaa agcttttttt aatttttcta ttgttccttc
    28741 tattccatca tcttgtttca tatttacagt aggataatct atagaatccc ctattatcat
    28801 ttttgcagaa tttaagactt cttcagccat attgccatga ctcatcaaaa taatactatt
    28861 caatttaata cacctcttta aatctataag atgtctacaa gataagtttt cttatcatca
    28921 ggcgtcatta tagttgtaat ttcaattcct tcatctaaaa gtcttttaaa tgcttctttc
    28981 tcttcttcag taacactgat attaggtctt agaactgttg tatcttttct acttgacata
    29041 tttcctacat ttatattatg tattttaaat cctgcctcta caaatggaat aaggtccaca
    29101 ggagacttaa caattaataa tacattttgt ccttcatact ttcctgcagt aatatttttt
    29161 atagcagtct cttctgtaat tatagatgac gctatacctg caggtgtagc cattcttaaa
    29221 acttgttttt gaacagaatt gttagctact gcatcattta ctaccattaa tcttgtaaca
    29281 cccaatttat tactccacat agttgcaact tgaccatgga ttaaacggtc atctatacga
    29341 atatttttaa tacctttcat ttccatatct aatatcatcc tttccttatt ttaaaatacc
    29401 taatgcatat aaaattattg aaacaaccat tactcctaaa attaagtata taggttttat
    29461 attcttttta agagcccaat aaacaatcac aactaaaatt gcaggaacta acccaggcat
    29521 aatttgatct aatatttctt gacctttcat tgtaaaatca ccttgttgaa agttcaaagc
    29581 aacattagct tttacaactg aaggtattaa tgcacctaca accattaaac ctaaaataga
    29641 tgtactctca gttaaaatct ttaaactttt ttcaattgaa gatattagct tagtacctga
    29701 tgtataacca gcattaaata gtggtatact taataatttc aatgctacac tcactacaat
    29761 ccacagtaag attccaaatg gacttccatc tatcgccata ttagctgcta tagctccaaa
    29821 aattgtccac ggaataacga caaataaact atctcctaaa cctgctactg gtcccatcaa
    29881 gcctgtttta attgaagcaa cagcctcttc agtctcttca cttgataact cctccattgc
    29941 tacattaata cccaaaatat aaggggcaac ccaaggatta gtgttaaaaa attgtaaatg
    30001 atttaataca gcactttgta acttttctgg attatctttg tatactactt ttaaaaaagg
    30061 tagtactgca taacagtatg ccaaggcctg catcttctca taatttgaag aaacagaact
    30121 agtaaaaaac cagcgccata agacactttt tttcaccttt tcatttgatt tatttaatga
    30181 taccttatca ctcatcttcg tccatacctc ctactacagt atattgttgt tcttgatttt
    30241 ccaaattctt tttgtaaatt ataagtgcaa ttgctccacc aattaaagct acacctaata
    30301 atggtacttt catatatact gctaaaacaa aaccaattac taagtaacta aaatactcct
    30361 tagcaggaag atatctaagt aataatccta tccctactgc tggaagtagt cctccagcta
    30421 ctgttaaacc acctgttaac cattcaggtg tatatttcaa aatggtatta attaaagaag
    30481 gtccaaatat tacagataga gcaactggaa tacctgttgt agctgctgtt aatagtactc
    30541 ctaacatttg catataggtt atatttttat aatttccttt tttagctcca tcttctgctt
    30601 tatgaactag ccatatacca atagtatttc taagaacatc taattgtacc atcaataaag
    30661 caactggaat acctatagag attcctacag atgcttcttg cttagtcgta atagttataa
    30721 atgtggctac tatacttgct gtttgataat caggtattga tgctccacca aagttactaa
    30781 tacctaatga taatagctgt aatgtacctc caatatataa ccctgtattt atatctccta
    30841 agataagacc agtaataaaa cctgctgtta ctggttgata tgttccaaac attgttgaat
    30901 ttttttcata gtttataaaa aatccatata gtacgattaa taataattgc cataattgtg
    30961 gctccattat gatttcacct ctcccaaaat attttttagt tctttaaaat tataactgga
    31021 atcttgcttt aggaaaatag gaatttcttc cagtgggcat tcaacttcat aagttttccc
    31081 accttcataa ctaactccac taaatatatt ttcccatgta tgtccttttg gtaaatatac
    31141 ttttctactt ctatctttat aattcattat tggagctact aaaatttcat ctccaagcat
    31201 atatgtatta tccacctccc aagttttatc atcttcagga aattcataaa ataaagttct
    31261 cataactggt gttccatctt catgcgcttc tttcatcagt ttttttaaat aagtttttaa
    31321 gctctctcta atttttatga attttgtaag aattacttca acttcttcac cataagacca
    31381 tatttcatta ggagctccag ttaccattga gcctccacct ttatttgata atggtttact
    31441 atgaggcaat ctatctccat gcattctcaa aattggtgaa aaagtagcat attggaacca
    31501 tcttaccata agttctctaa attctgggtc tttaggattt cctccatgga aacctccaat
    31561 atcagtagtc caccatggta tacctgccag tcccatattt aaccctgtat ttacttgatt
    31621 attaaaagct tcaaaactag aatcaatgtc accagaccat actaaagctc catacttttg
    31681 agctcctgcc caagctcctc ttacaagagt tacaacagac tctatgtcat tatgtaaacc
    31741 atcataaatc atttttaaat aatcaatagg ataaatatta ccacaactta aaacatttcc
    31801 ttttttatat ctatagttat caaaatcata cacagaatag ccaggttcag ctacatctaa
    31861 ccaataatag tcaatcccaa gttgtttata atttttatct attctatccc aaacatattc
    31921 tcttgcattt tcatttgtca tatctacaaa gactgtattt ccttgaattt gcattgtcat
    31981 tctaacacca cgattaacat tgactaaata gccattttct aaataatcat tgtagttttc
    32041 tgcatcactt tgaactgtag gccatacaga tactattggt tctactgaat actcttcttt
    32101 caatttttta actaattctt taggattttt ccaataatct aagtcaaatt tatattcacc
    32161 ttgcttaggc caatgaaaat agtcaattgc aattgatgaa agcttaattc ctcttttaga
    32221 atattccttc acaacatcta aaacttcttc tgaagttcta tatcttagtt tactttgcca
    32281 tagtcctaaa aggttttcag gcatcatagg aacagttcca gtaacttgag agtaattttt
    32341 atttaactct tttgggcttt ctccacaagt aatccaataa tctataaaat ttgtagaaaa
    32401 catcttccat tctgtcatat ttttagcaaa agatactttt ccaattcccg gattattcca
    32461 taaaagtcca taacctagac tagaaatata aaaaggaact gatatttgag aatttctttg
    32521 ggctaactcc aaaacagtat tctttaaatc taaaaattta tgttgatatt gtcccattcc
    32581 aaaaatcttt tcagaaggct cagattcaaa tctagtagtt acttcaaatt ctccactata
    32641 gcttggtcga tattctcttg attttaactt tagagtagag ttaaagtctt tggtaatctc
    32701 aattgtccct acatcttctc caccatcatc atgttttaca gctcttaatc gaatatattc
    32761 tttcaataat atttcatttt tatcattata aaaagtaatt ctatctctat ggtctaagac
    32821 agcttttatc tttccatttt taatgattgc acttccatct tcatttttat taattgtaat
    32881 atcatcttta tttaacttag gtttttcgtt aagagcatag ttttcatcaa caaaattttg
    32941 gtctacaaac gacctaatac gtaaagagtt ttttccccat gcttctattc gcaatagttc
    33001 attgtcaaat cttttttcaa tatagtcttc agatattttt atcacggcta cctcctaaat
    33061 caatggtata atattctatc tttgtgtaac ttaagttaaa taatgtcgtt tagtatcgtt
    33121 atttataaat taggttttat atataattca tttgtacatt aaactaactt accaataata
    33181 tactttattt gactttgttt gtcaatcgct ttccggattt tagtaaaatt ttctaatata
    33241 ttgctggaaa gttgttctta atatctttta tttatacttt taatatctgt gatgatacct
    33301 atatacatct agttttttta aaacacatac aggaataaca tttgcctatt atgtagataa
    33361 tatcggactt gaaaatatgt atagtgacct tataaataca gataataata tattagatat
    33421 tgttcaaaac aatggatttt ataattataa ataatttatg aaattattta agaaaatata
    33481 caattgtata ccaagtcaaa aacgacaaca aataaataaa actaaaaaag atgttgtctc
    33541 aaaatataga taaatttatt tttcattgct aaaatgatta aaatatattt gatttaatat
    33601 acttgattta atataaaaaa agagtgtctc ataaactaaa aagttcactt ttagacactc
    33661 ctttatatat attatctact tcttaattca tttattacct ttctacattt tctttcagct
    33721 agtggataca tctttataag tactaattac tccacttaaa aatggttcct gtactgtcca
    33781 tcagtcatat ttttctgcca aatataatgg tgtttcataa tgagacaata aatatttata
    33841 tctctactta tcttctctaa gctcgtcaaa tccaactata ggtatctcta aattatattc
    33901 tttaagtagc ttttctagct cttctataaa aatacctttc ccatccacca tttgtatatc
    33961 ctttatttta tccatcaatt cattgcaacc acttaactca ccaaaccaac gtttagaact
    34021 acatgtctta ttataaccac aacttggact gccatctatt gcaatgatac tttttatatc
    34081 ataatcattt tttaaatatt catcaaactg ccttacatga ggtattaaca tttgcctaca
    34141 ttgttctcta aaaaacaaat tgtcaaactg ttcttttaca tgtccccacc tatttattcc
    34201 atacataatc atttctgggc atggtagttg aattattcca taccctttat ttattaaatt
    34261 tatcgctata ttttgtgctc ccttaaattc tgacaatccc tccactttag aattacaatt
    34321 taatatacaa tgacatacta ctacaatctc tctatttctt ttcataataa tctccttagt
    34381 tattcttaat tttaatgata ctacatattt tgataattat attatttatt tgttctatat
    34441 ttccaatatt ctatatagaa atatacaatt agtaatcttt tataatctat actatgataa
    34501 atgtatatat ttttttagga ggtattataa ttggatacta aaaaagtcac attttcttca
    34561 atgtgcatag ttattaatat tgtcttaggt atggttatat ctatgataaa tataccatta
    34621 ttatttttag atacagttgg tactgtactt ggggctgtcg tattaggtcc tttttggggt
    34681 gctcttattg gtggatgtac aaacttagtt ttaggtgtta tatcaggtcc tacaaatata
    34741 ccttttgccc ttgtaaatat tgtccttggt cttattgttg gatatatttc taagaaaaaa
    34801 ggttttggtt ataaagaagc tattattaca ggagtaattt tatctattgt ttgtccttta
    34861 attggtactc ctatttcagt tttattgttt ggtggtttaa gtggaagtgg tgctgattta
    34921 ttggttggtt ttttagttca gtctggtcag aagatattta ctgctgcgtt ctttccaagg
    34981 attcttagta atatagtaga caaacctttg tcttgtatta tggtggtata ttttataagt
    35041 aaaatgccta aaggttttat aaaacaatat agtaaaaata cttctagttc atattaataa
    35101 atcatttatt atgtaag
//
LOCUS       DAYASV010000026        34862 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYASV010000026 DAYASV010000000
VERSION     DAYASV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.026"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            27..764
                     /gene="cas6"
                     /locus_tag="ACGI68_001316"
     CDS             27..764
                     /gene="cas6"
                     /locus_tag="ACGI68_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFH0137761.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPINFKKRVLKEKIRGFKVREYYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            769..2244
                     /locus_tag="ACGI68_001317"
     CDS             769..2244
                     /locus_tag="ACGI68_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137762.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENRLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMETNVKRIVDTVIKSN
                     IEIDEIDKLDLTQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYSFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     GNTRNLGKRMIDNGNLSNLKELLLDNISVLKQSTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGGEEYKMKNYMESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2264..3277
                     /locus_tag="ACGI68_001318"
     CDS             2264..3277
                     /locus_tag="ACGI68_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HFH0137763.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYKGSTQLITNRGFLSSEFGRKYYDEKEENEVPR
                     DENFPTSQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVIGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3293..4090
                     /gene="cas5"
                     /locus_tag="ACGI68_001319"
     CDS             3293..4090
                     /gene="cas5"
                     /locus_tag="ACGI68_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HFH0137764.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYIKMGKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVKLKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4117..6525
                     /locus_tag="ACGI68_001320"
     CDS             4117..6525
                     /locus_tag="ACGI68_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFH0137765.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKSTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGVNKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            6966..7913
                     /gene="prmA"
                     /locus_tag="ACGI68_001321"
     CDS             6966..7913
                     /gene="prmA"
                     /locus_tag="ACGI68_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFH0137766.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7932..8690
                     /locus_tag="ACGI68_001322"
     CDS             7932..8690
                     /locus_tag="ACGI68_001322"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFH0137767.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEIILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNSVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8692..9990
                     /gene="mtaB"
                     /locus_tag="ACGI68_001323"
     CDS             8692..9990
                     /gene="mtaB"
                     /locus_tag="ACGI68_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061712 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HFH0137768.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10033..10383
                     /locus_tag="ACGI68_001324"
     CDS             10033..10383
                     /locus_tag="ACGI68_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFH0137769.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10546..10725
                     /gene="rpsU"
                     /locus_tag="ACGI68_001325"
     CDS             10546..10725
                     /gene="rpsU"
                     /locus_tag="ACGI68_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFH0137770.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10755..11198
                     /locus_tag="ACGI68_001326"
     CDS             10755..11198
                     /locus_tag="ACGI68_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFH0137771.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11309..11806
                     /locus_tag="ACGI68_001327"
     CDS             11309..11806
                     /locus_tag="ACGI68_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFH0137772.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            11999..12562
                     /gene="raiA"
                     /locus_tag="ACGI68_001328"
     CDS             11999..12562
                     /gene="raiA"
                     /locus_tag="ACGI68_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFH0137773.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12775..13011
                     /locus_tag="ACGI68_001329"
     CDS             12775..13011
                     /locus_tag="ACGI68_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HFH0137774.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13024..14367
                     /gene="yqfD"
                     /locus_tag="ACGI68_001330"
     CDS             13024..14367
                     /gene="yqfD"
                     /locus_tag="ACGI68_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HFH0137775.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKDFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14306..15421
                     /locus_tag="ACGI68_001331"
     CDS             14306..15421
                     /locus_tag="ACGI68_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFH0137776.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15478..15939
                     /gene="ybeY"
                     /locus_tag="ACGI68_001332"
     CDS             15478..15939
                     /gene="ybeY"
                     /locus_tag="ACGI68_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFH0137777.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            15945..16664
                     /locus_tag="ACGI68_001333"
     CDS             15945..16664
                     /locus_tag="ACGI68_001333"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFH0137778.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16783..17184
                     /locus_tag="ACGI68_001334"
     CDS             16783..17184
                     /locus_tag="ACGI68_001334"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFH0137779.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17248..18141
                     /gene="era"
                     /locus_tag="ACGI68_001335"
     CDS             17248..18141
                     /gene="era"
                     /locus_tag="ACGI68_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFH0137780.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18153..19532
                     /gene="mgtE"
                     /locus_tag="ACGI68_001336"
     CDS             18153..19532
                     /gene="mgtE"
                     /locus_tag="ACGI68_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFH0137781.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19605..19760
                     /locus_tag="ACGI68_001337"
     CDS             19605..19760
                     /locus_tag="ACGI68_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HFH0137782.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19726..20499
                     /gene="recO"
                     /locus_tag="ACGI68_001338"
     CDS             19726..20499
                     /gene="recO"
                     /locus_tag="ACGI68_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFH0137783.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20492..21091
                     /locus_tag="ACGI68_001339"
     CDS             20492..21091
                     /locus_tag="ACGI68_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HFH0137784.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21251..22129
                     /gene="glyQ"
                     /locus_tag="ACGI68_001340"
     CDS             21251..22129
                     /gene="glyQ"
                     /locus_tag="ACGI68_001340"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFH0137785.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22129..24195
                     /gene="glyS"
                     /locus_tag="ACGI68_001341"
     CDS             22129..24195
                     /gene="glyS"
                     /locus_tag="ACGI68_001341"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFH0137786.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQNDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24361..25908)
                     /locus_tag="ACGI68_001342"
     CDS             complement(24361..25908)
                     /locus_tag="ACGI68_001342"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HFH0137787.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEKLFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNKEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26188..26955
                     /locus_tag="ACGI68_001343"
     CDS             26188..26955
                     /locus_tag="ACGI68_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFH0137788.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27238..27435
                     /locus_tag="ACGI68_001344"
     CDS             27238..27435
                     /locus_tag="ACGI68_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0137789.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27464..28540
                     /gene="vorB"
                     /locus_tag="ACGI68_001345"
     CDS             27464..28540
                     /gene="vorB"
                     /locus_tag="ACGI68_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0137790.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28543..29262
                     /locus_tag="ACGI68_001346"
     CDS             28543..29262
                     /locus_tag="ACGI68_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0137791.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29275..29814
                     /locus_tag="ACGI68_001347"
     CDS             29275..29814
                     /locus_tag="ACGI68_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0137792.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            29971..31029
                     /gene="buk"
                     /locus_tag="ACGI68_001348"
     CDS             29971..31029
                     /gene="buk"
                     /locus_tag="ACGI68_001348"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0137793.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31047..31940
                     /locus_tag="ACGI68_001349"
     CDS             31047..31940
                     /locus_tag="ACGI68_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HFH0137794.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            31970..33178
                     /locus_tag="ACGI68_001350"
     CDS             31970..33178
                     /locus_tag="ACGI68_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0137795.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33202..34461
                     /locus_tag="ACGI68_001351"
     CDS             33202..34461
                     /locus_tag="ACGI68_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0137796.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14072 a         3542 c         6442 g        10806 t
ORIGIN      
        1 tatactagaa agtaggaggt gaaaatatga gcagaatttc aatttttatg gaatcatgta
       61 atggcaaatt tccaaatgaa aattcaatgt tgtcagtttc ctttataaaa aatattttaa
      121 atgcagaaaa caaagatttt gctaaaaaca tatttaacta tggggaagaa agaaaaaaca
      181 ataagcaaat aaaggatata aatacagcca tttacatacc aaatcttata agaagcaaaa
      241 atgagttact tgttgatgga gatataatat tcaatataag tttttataat tattctatgt
      301 tcagtaaatt atacaatgga attttaaaag taaaggagct agaatataaa ggttatagat
      361 ttaaaataaa aaatataaaa atacataaag aacacgagat aaaagaaaat ggtgttatat
      421 ttaaaactat gtctcctata atagtaaaga ataaagaagg taaatattta gatgtagaag
      481 attcaaatta tatagaatgt ttaaactata tagctaatct aactctggag agtataagag
      541 gtagtgggct tagaaagcca ttggaattta tacctattaa cttcaaaaaa agagtgttaa
      601 aagaaaagat tcgtggattt aaagtgagag aatattacta tataaatgct tatgcaggaa
      661 catttttttt aaaaggagat atggaagatt taaatgcact gtataaaatg ggaattggat
      721 atagaaggac agaaaatgca ggaatggttg atatattaaa gtagcgaggt gagagaaata
      781 aaagctaaat tttatagatc aggtgatatt gtaagagata ttggcattgt ttacttttat
      841 gaattactaa tggatttaaa aaacgaacta gaaaaaaatg attataagct aaaatttact
      901 tgtgagttaa atagaaatta tttatcttta gaatctgcag aatatataga ccctaagtat
      961 atatctgatt atatattaga aaatcgactt tttaaggtat ttaaaaatgg gaaaaaggaa
     1021 accttagaaa aaatatttag taatatagat aatttaacgt atgtaaattt tattgaagaa
     1081 ttagataaat cttctgcaac tgctaaacaa aaggaagata ttaaaaaaga ttttaaaaac
     1141 aggaggtttc catatgttag aaatagtggt aagtatggat taaacacgag tttagaaaat
     1201 atggaaacaa atgtaaaaag aatagtagat actgtaatta aatcaaatat agagatagat
     1261 gaaatagata aacttgattt gacacaatat gaagaaaaag atagtgtatg taatgtttgc
     1321 aatataaata aaacaacaaa actagatatt gatttaagag aacgaaagga ctctaagtat
     1381 aactttttat ttagaggtgc agaaaaatca ggctttaaaa gaagtggaca agttgaaagt
     1441 aatatttgtt ttgagtgtga atttttcaac ttaatgtgtc ttttatatat aaatttaaaa
     1501 agaccaatgg tttttgcata tacgaatgat ttaagagaat tagcattttt aaatcataag
     1561 ataatgctta aaagaaaaat gtattcagat aaatcatttt ataaaaaact tttgcatgaa
     1621 aaaataagtt caattagact atatagcttt gatattgata cgaataaagg tataattcta
     1681 aagtttgatt ctattataga gtataaagaa ttattaaaaa aaattgaact tatagatatt
     1741 atagataatt ataatttttc aagagagcca ggtaatacta gaaatttggg taaaagaatg
     1801 attgataatg ggaatttatc aaatttaaaa gaattattac ttgataatat aagtgtttta
     1861 aagcaatcaa ctggaacttc tcgagaaatg gattttgtat ctagctcata taatatcggt
     1921 ttatatataa aattgtgttg gattgtagat ggaggggagg agtataaaat gaaaaactat
     1981 atggaaagta atttaatata ttcaagagta ggtaaagact tatttaataa aatgacagat
     2041 gaatccagaa gaaaaaattt ttctatgaaa gtgattcaac tacttaagtc taatgataga
     2101 actcagctat ttcaaactat aatgcatgtg ttagtttcta atggaatctt aataggagaa
     2161 gggtttgtag aaggtctaat gcagagttct gaattagaat taaatacgaa tgtaggtcta
     2221 tttattcaag aaatcatgaa ataaaaaagg gggaaaattt aaaatgaatg aaaaaagagt
     2281 aaaaggagta acattaacta tactgacaga aagcccagtt ccactttcat atgaccaagg
     2341 gtatggaaat tatacaccaa taaaaaaaga acaatataga gaaaaaatac atgcaaaaac
     2401 tagtatagcc acaataacat atgatttaag aagaatgctc caccaagaat atggttggaa
     2461 tttaagtaat attgtttttg gaaaaaaagg aaatatatat ccatctatta agaaaaatgt
     2521 aggatgtgta gatgaaaatg gattagagac tgatgtattt ggttatttaa taccacttaa
     2581 agataaaggt tctataagta aagcttctcc acttagaata atacctttta ggtcattaaa
     2641 tccatataaa ggttcaacac agctaataac aaatagaggc tttttaagta gtgaatttgg
     2701 tagaaaatat tatgatgaga aggaagaaaa tgaagtacct agagatgaaa actttccaac
     2761 atcacaagca ttagcaatag aagagacttt atcagattac tacgtttata caataacatt
     2821 ggagcttgat agaataggag tagtagaagt agaagatgga aagttacttt taccagaaga
     2881 aagaaaattt atgagtaagg agttaagaga aaaagcagta aaagatatat tagatgcaat
     2941 tactgtgttt acaagaaaca taaaacattc atcagtacta ttaaaaccac ttgcagtaat
     3001 aggtggagct tttgataaag ttgtaccatt tttttgggat gatgtagatt ataatgctga
     3061 tagtggtgag ataaatttag agggagtcat agaaactata gaaagttata gccttaaaga
     3121 aagtaacaca atacttgcta tcaatgatag attaaaaatt tctaataaaa aggaattaaa
     3181 taagtttaat ttaagtaaat accctgtaaa agagattaaa aatctagcag atagattgga
     3241 aattggagaa gataatatgt ggtatttaaa agagtaggag gttttttgaa ttatgaaagt
     3301 attacaatgt aaacttaagc aaccaactgc tcactataga gacccaaaag tatttcaaaa
     3361 tgaatatata agtactttaa atttaccatc taagaccaca ataatgggaa tgataacgta
     3421 tttatgtgat agaagattaa actctgatat agatatagga ataattggaa cacatcatca
     3481 tagagaacta gaattttcaa gaggagaaaa cattgatttt tggaatgaat atataaaaat
     3541 gggaaaaggt aaagataaag aaaagttttt gcttaaagga aactactatg attactataa
     3601 agagcataaa gctcaaaata gcatattaaa ctatgaagtt cttaaagaag tagaactgac
     3661 tatatttatt tcttgcaagg atgatgaaga acttgaattt attagaaaaa agcttgaaag
     3721 tccatgtaaa tatgcaaatt taggaagaaa agaagatttt gtaataccaa gtgaaaaagg
     3781 gtgttttgtg aaagaagtta agttaaaaga ggttatacct atgaatactc gtgatgctat
     3841 aaaagaaaat ataaaattaa agaatacata tgtaagagtt gacttaagag ataaagagaa
     3901 tgtcgagact ataataaatc aaggtgtatt gattgcattg cctcataaat ataaagattt
     3961 agaagcaaat agagatgata gagtttatga gttttgccat tatatatatg ttgataatga
     4021 tggtatatat cctaaaaata ttaaggtaaa tgtatgtatt gatacaaaag aagtttttac
     4081 atggctttaa attattttga aagataggtg tttatcatga aaaatatcat gcaaaaagaa
     4141 atattagcta agtcatacaa tttaagaact aatcaacaag aacaaacatt agttgaacat
     4201 attaaggatt tatttgaagt attagaatct atattagaat taaatctata ttcagataaa
     4261 gatgtagaga tactaaaaat atgttgtgct ttgcacgatt taggaaaaat taattcaatt
     4321 atgcaacaaa aaatggagat taataataaa atatcgtata gttgctcaga agaagagagg
     4381 aaaaagttag agtctgacaa aaaatcttta aaaaagatag caagacataa tatattttct
     4441 ggagcatttt taaaagatat attagaaaaa atgaacttgt cagaagaaga taagttatat
     4501 atatataaaa gtataatgtt acatcatgga aactatgaag attatatgag gttaagtatt
     4561 agtaaagttc aagaagaaat ttatgagtat attgaaaaag gcattttaga aaaagaagag
     4621 tttaatctga aagatataga aagttatata aatagcattt taaatgtaga ctttaaattt
     4681 ggtgaagatg tattggatta tgattacata gataaattaa gtgaaagtat gtttatagac
     4741 agtgattata acaatggtca aatagatgat tcagttttaa attatagaaa gtttaagtat
     4801 attttatata aagggatgct gaacttaata gaccatagtg catcaagtag acagaaaggt
     4861 ataaaatttt ataacgattt tactgatgaa gaaattgata atatgatttt aaatgagata
     4921 tataagagtc agggtaattt ggaaaaaaat aatatagagt tcaatactat acaaaaaagg
     4981 ttgagagaac tttctggaag aaatgttctg actgttgcat ttacaggttc aggaaaaaca
     5041 gctgcagatt atagaaagac ttttaaaaga aaattctttt tagtgccaaa taaaatatca
     5101 gcagaaagtt tttatagaaa aaatattttt caaaataaaa atgatttaga tacaagaagt
     5161 agtaatgatt acattggatt acttcatgga gatataaact tatattcaga gaatgaagat
     5221 aatggagaac atgattttgt attgacatta agggatatag atttgagtat aaacttttgc
     5281 aaaccttgtg taattgcaac agttgatcag ttattactta gtatgtttaa atttccggga
     5341 tatgaaaaaa tattcgcagc cgtaaaaaat gcatctataa ctgttgatga agtacattta
     5401 ttggaaccaa agatgttttt gattttgata tattttatgc agtttgcatg caagtactta
     5461 gatgttgatt ttcacttaat gacagctaca cttccaaaat catataaaga gcaaatgata
     5521 aacaagggaa ttatttttca agaagaaagt aatgaaaatg ttactgataa aggagaaata
     5581 gtttttattg agagcaacaa ggaagaagag atttgtgaag gtaaagatgt aaaggtttct
     5641 tttataaagg aaaaagaaat caagtcaata gttgaagatg ctttagaaaa taaacaaaaa
     5701 attcttataa taaagaatac tattgattca gtcaatagaa catatgagtt tttaaaagaa
     5761 aatttatctg ataagtatgg tggtgtagat atagatgttt tacatagtag atttaagttc
     5821 aaagataaaa aagagaaata tagtaaaata ttaaatggag agggtgatat ttggatatca
     5881 actcaatcag tagaaatatc cttggatttg gatttcaata taataataag tgatttagca
     5941 actatggata gcttaataca aagaatgggt agatgcaata gaaataataa gtatgaatat
     6001 ggaaactttt atattctacc tagtgaagat aaaatttatg acaacaaact taagagcaca
     6061 actaaaaaca tcttaaagga catattaaaa actaactcta tttttacaat gggaattaga
     6121 aaaactattt tggatgatta ctatgataat agtgtagtca aaaaatattt tgaagagaat
     6181 tttataagtt gtgataaaga aattaagaat atttatggtg taaataaaga aatttttgat
     6241 ggattggatt taatttttaa ttttgaacct tacaaaaata ttgttgatag taagaaggaa
     6301 gcagctaaaa tttttagaga tgttgatgtt agttacaaaa ttattctgga agaagatttt
     6361 tataaggaag atagagagtt acaacaagat tcgattcaag tatcagggtt tatatttaat
     6421 agattatatt attatgggtt aataagcaaa gtagaaggat acatggtact aaaatctagt
     6481 agtaagtttg agtataatag tactgtaggc ttaattttga agtgagcata tgtttaaata
     6541 taatatacct ttagacattg aaaatacaaa aagtactact gtaagctcac tgtaaaaact
     6601 attttttaaa aggaaaaata agttgtgaga tagagtgttt acaagtgtat atagataata
     6661 aatttgaggt ttaagattaa ctaagtgggt ttcaggtaca tcgcgaaaat aattataagt
     6721 tggagatttt gtatgtttaa gattaactaa gtggatttca aatattgata tagtagatat
     6781 aatagaagtt ttattattag gtttaaaatt aactaatcgg atttttaaat attctgacta
     6841 ttatataagg atattattag attgaattta aaaccaataa tataaaacaa caaaatatta
     6901 tatttatgat ataataaaag ttatggaaaa acaggaatat tactatagag aagagaggta
     6961 aacttatgaa taattggata gaagtaacta taaaaacaac aacagaagca gttgaaccta
     7021 taacgaatat actatatgaa caaggagctg gaggagctgt aatagaagac ccaaaagact
     7081 ttttattcca aaagaaaaat gaactagact gggattatgt tgaagaagaa gtatttaaaa
     7141 agaatgaaga agatgatgtt cttataaaaa cttatgtttc tgaagaaaaa aatgtgatgg
     7201 aatttgtcga aataataaag caaaaagttt taggattaaa agattttggt atagatatag
     7261 gtgaaggaag tgtatcattg gaccaagtaa atgaagctga ttgggcaaat gcatggaaag
     7321 catactacaa accaacaaaa gtaggacaaa gggtagtagt aaagcctaca tgggaagatt
     7381 atgctatgca agagggggat ttaatcatag agcttgaccc tggaatggca tttggtacag
     7441 gtacacatga gactactagt atgtgcatta gagaattaga aaagtatgtg aataaagatt
     7501 ctaaagtatt tgatattgga tgtggtagtg gaatccttgc aatagcagca gctaaattag
     7561 gagcaaaaga agttgtagct gtagatttag atgaagtggc tgtaaaagtt gcaaaagaaa
     7621 atgttcttga aaataaagtt gaaaaaagtg tatctgtaat gcatggtaat ttaactgatg
     7681 taataaaaga taaagcggat gtaatagttg caaatataat agctgatata ataaagatac
     7741 ttgcaaaaga tgttcaaaac tttatgaaag aagatgctat attcatatct tcaggtataa
     7801 ttttggataa agtagaagaa gttaaagaaa gcttaattga aaatggattt gaaatagttg
     7861 aagttcaaaa attaggtgaa tggtcagcaa tagtttctaa gcttaaaaaa taagggatat
     7921 ggtgacgtat aatggataga ttttttgttg aaaaaaataa tataaatcta caagataaaa
     7981 cttgcactat agaaggtgaa gatgtaaaac atatttcaaa agttttaaga tgcaaattag
     8041 gtgagaaact tgaaatatgt gacaagaata ataatgagta tatttgtgag ataatgaata
     8101 tagataagag tatagttaat ttagaaattt tagaaaaagt tgatataaat agagaatcag
     8161 aacttaaagt aaggctatac cagggacttc caaaagctcc aaaaatggaa ataatacttc
     8221 aaaaattaac tgaagtgggt gtagaagaaa taatattagt tcaaactaag agaagtgttg
     8281 ttaaagttga tgacaaaaaa gaagataaaa aatttgaacg ttgggaaaga ataatttatg
     8341 aagcagcaaa gcaaagcaaa agaggtaaga taccaaaatt aagaggtgtt ttaagtttta
     8401 aagaagcttt agaagatatg aaaaaaaata gtgtaaatat ttgcccttat gaaaatgaaa
     8461 gaacagtttc aataaaacat gcactaaaaa aatgtgattc taatatagat agtgttggaa
     8521 tttttatagg tccagaaggt ggattttctg aagaagaaat agaacaaata caaaaaaata
     8581 attgtaatgt tgtatcattg gggcctagaa tattaagaac tgagacagct tctgttgtag
     8641 catcaacaat agctttgtat gaactaagtg atttaggagg agaaaaataa attgaaaaaa
     8701 gtagcttttt atacacttgg gtgtaaagtt aatcaatatg aaacagaagc tatgctagaa
     8761 ctatttgaga aagatggtta tgaacaagtc aatagtgaag agtatgctga tgtttatgtt
     8821 ataaatacat gtacagttac tcatatgagt gatagaaaat ctcgtcaata tataagacga
     8881 gttaaaaaga agaatccaga tgcaataata gccgttgtag gatgttattc tcaggtatct
     8941 ccggaagaaa ttttggatat agaagaagta aatttagtca tgggtacaaa tgatagaaga
     9001 aagattgttg aagaaattaa aaagataaat tcaagtaaga aagttagtac agttgatgac
     9061 ataatgaaag ttaaagcttt tgaagaaata gaaatcagcc aaactaatgg aaagactaga
     9121 gcatttatga aaatacaaga tggatgtgac agattttgta cttattgtat cattccttat
     9181 gcccgaggac gcgttagaag tagagatatt gatagtatag tcgatgaagt taaaaaattg
     9241 gctaataatg gctataaaga ggttgtatta acaggaatac atgttgcctc ttatggaaaa
     9301 gatttaaagg atagggatat taaacttctt gatgtaataa aacaaataaa ccagatagaa
     9361 aaaatagaaa gaattaggct tagttctgtt gaaccaattt tatttacaga tgaatttgta
     9421 aatgaagttt taaagatgga taaagtttgt cctcattacc atttatcact tcaaagtgga
     9481 tgtgatgaaa ctctaaaaag aatgaataga cgatacacaa ctttagagta caaaactatt
     9541 gtagatagat tgagaagtaa aatgccagat gtggcaataa ctacagatgt aatagtaggt
     9601 tttcctggag agacaaatga agagtttaaa aaaacgtacg agtttttaaa agaaatagag
     9661 ctttcacaaa tgcatatttt taaatattca ccaagaaaag gaactccagc tgcaactatg
     9721 gaaaatcaag ttgaccctca aatgaaacac tttagaagtg aacaactttt aaatttaagt
     9781 aaagtaaatt ttaataaatt tgcaactaaa tttatcggcc gtgagttgga tgttttattt
     9841 gaacaaaata tagaaggcaa taaatatgag ggattaactt caaattatat aagagttgtt
     9901 gttgaaagtg acaaaaatat acaaggtcag atattaaaag tgaaaataaa tgatgtaaaa
     9961 gatgaatatg tagaaggaat tttgctataa catgtagaaa ttataatatt tataagatag
    10021 gaggtggaaa gaatggattg tattttttgt aaaatagcga acggagaaat accttcaact
    10081 aaagtttatg aagatgatag agtcttagct tttaatgact tgaacccagt tgctccatat
    10141 catatattag ttgtacctaa aaaacattat gatagtttaa ttgatattcc tgataaagag
    10201 atggacattg tgtcacatat tcatgttgtt attaataaaa tagctaaaga gaaaggtttt
    10261 gaccaaactg gattcagagt cataaataat tgtggaagtg atggagggca agaagttaaa
    10321 catttgcact atcatatttt agctggtaaa aagcttccta attacgaagc gggacaaaac
    10381 taaactaaat tattgaaaaa ttgagttcct tcttgataaa aatacttgat aaaagtaaaa
    10441 atttagttta taataaacaa agtgttaggt tgtaatgcat attatgcaaa aagaacatag
    10501 ttacagtccc agcataattg cgctattgga gggagggaaa aagaaatgtc agaagtaaga
    10561 gttagagaga atgaaacatt agacagcgct ttaagaagat tcaagcgtca atgtgcaatg
    10621 tctggcatca tgtcagaagt gagaaaaaga gagcactatg ataagccaag cgttaagcgt
    10681 aagaaaaaag ctgaagcagc aagaagaaaa aacgctaaga aatagtagta aataagaata
    10741 aagaggtgaa ggggatgtcc cttaagcaaa agttacaaga ggatttgaaa tcttcgatga
    10801 aaaacaaaga tacagtgaga aaatctgtag taactttaat aagagcttct ataaagcagt
    10861 atgaggttga taatagggtc gagcttgatg aagatggaat tatcgatgta atagcaaaac
    10921 aattaaagca acgtagagat gcattagttg aatttgaaaa agctggaaga gaagacttaa
    10981 taaaagaaac agaaggtgaa attgaagttt taaaagagta cctacctcaa caattaagcg
    11041 aagaagagtt agaagaaatt gtaaaatcta ctatatccga agtaggggca acttctatga
    11101 aggatatggg aaaaataatg tcagttatcc aaccaaaagt taaaggaaga gctgacggaa
    11161 aacttataaa taagttggta aaacaaaact tacaatagat tgatatagcc tagagagaaa
    11221 tctctaggct ttttatagtt tttgggaata acgagtcata tttgagcata tctataatca
    11281 aagaaaattt tgaaagaagg ggaaaaattt gattatatca gtaaaagaga ttaggaattt
    11341 tttgataaac atatttatac cactaataat aggttactta agtagcatct taacaatgat
    11401 tatcagtgga acagatattt caacctatta tttacaactc acaaagccaa gctttgcacc
    11461 accagctttt atctttccta tagtttggac tatattgtac atattgatgg gtatatcttc
    11521 atacctaata ttaagaaagg gttacaactt acctaaagta agagatgcaa ttttttatta
    11581 tggattacaa cttgtattaa actttatttg gagtatacta ttttttggat ttggacttag
    11641 atttacagct ttgatagata taataataat gattttggtt tctataataa tgatttcgaa
    11701 gttttctaaa atagataaga gatctggata cataaatttg atttatttaa tgtggcttgt
    11761 ttatgcagga tttttgaact attttatatg gtttattaat aaatagaatg taaagatttt
    11821 gtaaattacc ctgtactttt aataatatca taacaaacga aaaataaata gactattaaa
    11881 aataatcatt ttcatataca ataatattaa tttaaataca ataaatttat aaaaattgta
    11941 tttaaagatt aacatattaa agtattagaa ttggaaaggc ttgaagggag aggtttttat
    12001 gcaaataata gtatctggga gacaaatgaa attaactgac ggaataaaag gatacgtaga
    12061 tggaaaatta agtaggttag aaaaatacct tgatcctgaa tcagaagtga aagtaacagt
    12121 aagtgcgaaa aaggatagac aaaaagtgga ggttacaata attcctatta acggtcaaat
    12181 aataagagca gaagatgtag aagatgactt atatgcagca attgatattg tttgtgacaa
    12241 attaagtaga caagttgtca aatataaaac taaagttaaa gacaaagttc aaaataataa
    12301 gagcataaga tttgaaaatc ttgactttat agataatagc agtgaatttg atgattatga
    12361 ttatgatgat gaagaagatg aaaatatagt aatagaaaga agaaagaaat ttaatgtaaa
    12421 acctatgagt tctgaggagg cgatacttca aatggaacta gtaggacaca atttctatat
    12481 gtttagaaac caagataatt ttgaaataaa catagtgtat aagcgtaaag ctggtggata
    12541 tggtttaata gaacaagatt aataaaaatt gtatacgttt agattttata taagaaaaaa
    12601 taaaaaggta atcatgttga ttttttatca atatgattac ctttttcttg tttaaataat
    12661 atattagata ttaggtattt attatataaa aattttaaaa ctaatctgtt acaacatcaa
    12721 taaagtgcat aaatacctat ttcaagcata aaattaatta agggggtaat gcttatgtta
    12781 gaaatatcca ctgatttaca agtaaatcaa ccagttatta ctgttacaag taatacgttt
    12841 attagtatag aaaattatct atcaatatta gaatatgaag ttgatttaat aagaataaag
    12901 actaaagtta aaacaattaa aatatctggg gataaacttt cacttaagta tattactgat
    12961 agtgagatag gtataaaagg gattatctac aatgttgagt atgttgatta ggaggagata
    13021 cacttgataa gttttataag gggatactat gttatagttg tggaaggagt aggactcgaa
    13081 caatttttaa atcatcttat aagaaatggg ataagtgttt acaatgtaac tagaataaaa
    13141 aatactaaaa tggagtttca tatagataga caagatataa aagactttaa gaatgtttat
    13201 agaggaagta agtttgatat aaaggtaaag caaaaaactg gggtcccttt tattatcaaa
    13261 agagtttata agcacaaagg tatgtggatt tgtgctcttg tttctttatt tttattgatg
    13321 tctacatcac agtttgtgac agatgtatat atacaatctc ctgaaggtat aaaaaaagaa
    13381 gctcttagaa atgagcttta taaggttgga gtaaggccag gagtctataa gaagagtatt
    13441 gatagaaaag aagttagaga ccatatgatg tcaaaattta atgatgtggc atatttatct
    13501 atcaatgtaa aaggaacaaa tatatttgtt actgttacaa aaaaggcaga atccctaaaa
    13561 tctacagacc aatcaaatta ttgtaatgta attgctttaa aaaatggtat aatagaaaag
    13621 gtaatcccta gaagtggtaa atcagttatt aaatctggag acatagtcca aaagggagat
    13681 gtcttattaa atggagctaa taccaagtca ataccagaag tatgggcaag tactttttat
    13741 gagtccacaa aaaaggcaag ttatgtagat actgtaaata aaaaaactgg agaaaagaaa
    13801 aatatatata cactaagttt ttatgataaa gagtttacaa tgagaaaaaa tataaaatat
    13861 aaagattatg ttgttgaaaa taaagaaaaa aaactatcaa taggaaacta tacttttcca
    13921 ataaagataa aaacgagtac tttttatgaa acaaaaaagg ttgaagtaaa aaggaataaa
    13981 gaagagttaa aaaaagaatt atctgaaaaa gctttaaaag agttagaata cataatccca
    14041 gcttcggcta gaataataga tgtaaaacat aactttaagg ttaataaaaa tatgttagaa
    14101 tatttaataa cagttcaaac tagtgaaaat attgctaaaa tttatcctct tagtaagtca
    14161 gaagcagaaa ggtttataaa ggaagaaagt aaaccagatg aaggtgaaga ggaagttcct
    14221 tccaatccag aaaaaagacc attaaatgat ataagaaatg agtttgatga ggataataag
    14281 gataataaag accaaaacaa ttctgatgaa aataatagta atcaaaatag taataatagt
    14341 caaaataata atagcaataa caactaataa aattaaactt aaaatcttag gaggactaat
    14401 tcaattgata atacaaaaga aatttacaat agcagatggt aactttgaaa gagagttatt
    14461 tggcaacttt gatgaaaatg taaaattaat agagaagact ttaaatatag atgtaatact
    14521 tagagaaggc aatataattt taattggaga agaaaaaaat gtagattctg cattaaaact
    14581 tatgaatgaa ttacaccaaa ctgtatcaaa tggtaagcat ttagataagc agagtatttc
    14641 ttattcatta tctcttttgc ttgaaggaag tgaacaaaaa ataaaagaac ttgaggggac
    14701 aatcgtcata actcaaaggg gaaaagctgt tcaacctaaa actttaggtc aaaaagaata
    14761 tataaaactt atagaaaata atgatataac ttttggtgtt ggacctgctg gaacaggaaa
    14821 gacttatctt gcggttgcta tggcagtaaa agcttttaaa agagatgaag ttagtagaat
    14881 tatacttaca agaccagctg tggaagcagg agagagtcta ggttttttac caggagattt
    14941 aaaggacaaa gttgacccat atttaagacc actatatgat gccttatttg aaatgttggg
    15001 tgctgataaa tttaataagt atttagaaag aggtactatt gaggttgcac cacttgcatt
    15061 tatgagaggt agaacacttg ataattcatt tataatattg gatgaagctc aaaatactac
    15121 ttctgaacaa atgaaaatgt ttttaactag acttggattt ggctctaaag ctgtagttac
    15181 aggagatgta actcaaacag atttaccaca aaataagaag agtggtctaa tacaagctac
    15241 agagattctt aaaggtgttc caggtattgg gtctataatg ctaacagata gagatgttgt
    15301 aagacatgag ttggtacaga gaataataag agcttatgag aagcacgaca aaagagaaga
    15361 atttaaaaaa gaagaacgta aaaaagcgaa aatgcaggaa aagactttca aaagaaagta
    15421 aaagtgtagt aatatagata tatagcatta ttaatgaata aaaagtaggt gtagtgaatg
    15481 gatttgatac tagatgatag acaagataaa ttagaagtaa gtgaggagtt aattgaaaag
    15541 ataaaggata taatcataga atgtttagat tatgaaggat atgatgataa ttatgaagtg
    15601 agtctttctt ttgtagataa taaagagatt catgaattaa atagagaata tagaggagtt
    15661 gatagagtga ctgatgtttt atcatttcct cttttaagtg atgattttga agatgttgag
    15721 ttagaagaag aatccctagg tgacatagta gtatctcttg aaagagcctt agaacagagt
    15781 atagagtata accatagttt tgaaagagaa gtatgttttt taatatgtca tagtatgttt
    15841 catcttttag gttatgacca cgatacagat gaaaatacaa aagaaatgag agaaaaagaa
    15901 gaacacatat taaataagtt aaacataaca agggagtagt ttttatgaaa ccagaaaaaa
    15961 ctcgtcaagg tataattaaa gcatttaatg cagctataga agggatattg tatacattta
    16021 aatttgaaag aaatatgaaa atacattatt taggttcagt tgctgttctt ataataagtt
    16081 tgttttttaa cttttcaaaa ttagaaatga taatgttact tatgtccata tgtttagttg
    16141 tagttgctga aatgtttaat acagctatag aaaaagctgt tgatttagta actgatgaat
    16201 atcatgttct tgctaagata gcaaaagatg ttgcagctgg aggcgttcta gtggcagcac
    16261 taaattcagt agtagttgga tatattttgt tttatgataa attgacagat atatctggaa
    16321 tattaattta taaaataaga gaatcagaat tacatataac cttgatatgt atattattgg
    16381 ttcttatagc tgttgtagtt gtaaaagcat tgacttctac aggtactcct ctaaaaggag
    16441 gaatgccaag tggtcatgct gccttagcat ttgctatagc aactgcaatt acacttatga
    16501 ctgaaagagt tgttgcctct actcttgcat atataatggc tgtattagtt gcccaaagta
    16561 gaattgaagg aaaaatacat acattttggg agactattgc tggagctttg ttaggtgtgt
    16621 tgatagctat attagtattt cagcttggaa tgttctataa ttaaaataaa tgtacataaa
    16681 atatttaaaa taaaacaaat ctgtgctaat taaattttta tgatataatg tagagtatta
    16741 aataaaaata acttttaatt attgtataca ggaggagttg ttgtggataa catagaattg
    16801 ttaaggttgg cagaagatgc cagacaacat tcttatgctc cttactcagg ttttagagta
    16861 ggtgcagctc ttttaactaa gagtggtaaa gtttatactg gatgtaatat agaatgtgca
    16921 tcattaggtg gaacaaattg tgcagaaaga acagctattt ttaaagcaat atcagagggt
    16981 gataaggata tctataaaat agctatagca agtgataact cagaaaataa tgagcaaaca
    17041 tatccatgtg gaatatgcag gcaggtaatt atagagtttg gttctgatat aaaaataata
    17101 acgggatata ctaaaggaga ggtgtttgag catagtataa aggatttgtt gccaaactat
    17161 ttctctggaa atgatttaaa ataaatttaa aagtataaat aaatttaagt aattaaaaca
    17221 agaagataaa aagttaggag acagttaatg ttcaaatcag gttttgtcag tatagtgggt
    17281 agaccaaatg taggaaaatc tactttaatg aataatgtag ttggagaaaa aatagctata
    17341 atgagtgata agcctcaaac tacaagaaat acaatacaag cagtatatac agatgaagaa
    17401 atgcagatag tattcttaga tacaccaggt attcacaaac caaaaaataa attaggtgaa
    17461 tttatggtaa aggcagcaac agaagctttt aaaaatgtag atttaatatt atttgtagtt
    17521 gatgattcta aaaaaatagg tcctggagat agaaagataa tagaggattt aagaagtgtt
    17581 aagacaccaa taattttagt tgtaaacaaa atagaccaac tagaccaaaa agatgagcta
    17641 tttgatataa tcaaaatgta tgatagagaa ggtattttta aagaaatagt accaatatca
    17701 gcattaaaag gaaagaatac ggatacctta ataaaagtta tacaaaatta tttagaagaa
    17761 gggccaaagt attttccaga ttatatgatt acagaccagc cagaaagagt attaatagca
    17821 gaacttataa gagaaaaagt tcttcactat cttaatgatg aaatacctca tggtgtagct
    17881 gttgaaattg aaaaaatgaa agcgagaaat gataaggaaa tagtagatgt atctgctgta
    17941 atatactgtg aaagagattc acataaggga ataataatag gaaaaaatgg taggaaacta
    18001 aaaggaatag gtaagtcagc aagacaagat atagaattgc ttttaggttc acaaataaat
    18061 cttcaattat gggttaaagt caaggaaaat tggagaaatt tacaaaatta tataaataat
    18121 tttggatata atgataaata aaggtgggtt atatggatag gaaactggat gcttcccaag
    18181 atttattata tgaagtaaaa tcattaattg ataataataa agtattggag ttaagagaat
    18241 taatagagga atatcatata attgatatat tcgatataat ggaaaattta gaggaagaca
    18301 tgaaaattca attatttgaa gtacttcctt tagatatggc atcttctata ttagaagaag
    18361 gtagtgtaga gttttttatt agtattttat ctaaactgga tgtagaacac tcgaaaaata
    18421 tacttgaact tatgtctctt ggagatatgg cagacaagtt gagtgaactt gaagaagaag
    18481 aaagagaaca cataataaat cttttgaatc aagagaatgc cgattatgta aaagagttac
    18541 tattctatga tgaagattct gctggtggaa caatgaccac tggatatatt tctataaata
    18601 aagatatgac agctcttgag gctatagacc atatgagaga agaagctgaa gaagctgaaa
    18661 ctatttatta tatatatgtg gttgatgatg aagaaaaact agttggagta ttgtcattaa
    18721 gagaattaat aatagctaga gacgcaaata tagtagaaga tttgatgagt gaaaatataa
    18781 tctctgttta tgtagatgaa gatagagaag aagcagtaag attagtatct aaatacaatt
    18841 taatagcaat tccagtagta gacagacaag aaaaattaaa aggaataata acagtagatg
    18901 atataattga tgtaatggaa gaagaagcaa ctgaagatat gtataaattt gcaggaagtt
    18961 cagaacatga aagagaagtt gcggaaaaag aaaaccctac attgagagag caaataatat
    19021 cagcacttag aggacgatta ccttggttaa ttattacctt agttggaggg ttgttggctt
    19081 cgttaatact ttctaactta gactatataa tgaatcctgt atatgctcca ttagtatttt
    19141 ttatacctgt agtaattggt atgggtggaa atattggaac tcaatcatca tctgttacag
    19201 taataacatt gtctaataag gatttaaatt ttagcaatgt ggttagagaa ggtattgtag
    19261 gtattattac aggtcttttg tgtagtataa taacaggtat tgtaatttac tttgttatga
    19321 gaaagttaga cattgtatta attgtttcaa tatcattatt tataaatatg gttttaggtg
    19381 caacaatagg tgcgtttatg cctgttttat taaaaaaaat ggatgcagac ccatctacag
    19441 tttcatcacc aattatatct acagctcttg atataactgg tatagcagtt tactttataa
    19501 taacaacggc attattgtca aaaattgttt aataacttta tgtatgattt atcctcttct
    19561 tactaaaaat ataagtataa atgtaagaag ggggtttttt aaaaatgaac aatatcagtt
    19621 gggaagtttt taaaaaaaca ggtagtatag atgcatattt atatttctgt gattgcaaaa
    19681 acatacatga agaaattgaa gaagtaaggg agaaaaaaga agacgatgat aatcctgaac
    19741 acccagggga tagtacttaa agctataagg tataaggaaa gtgatataat acttacacta
    19801 tttacaagaa agcttggaaa agtatctgca atagcaaaag gagctaaaaa aaataagagc
    19861 tctttgcttt catcatcaca gttattttct tatagcaatt ttaccttaaa aaaacaaggt
    19921 aatatgtaca aggttactca aagtgaaata ataaaaagtt tttataatat atcctatgat
    19981 atagaggcat tttcttatgc tacatatatt acaaaattag ttgaaaattc tatacttgaa
    20041 aatcagacta ataataggct atttatatta cttgcgcaga ctctgtattt gtatacacag
    20101 gataatacag acaataggtt tataacagca gcattcgagt taaaattttt agactatata
    20161 ggatttaaac ctatagtaaa taaatgcatt aattgtgaat gtaaaatttt acaaaattct
    20221 gtgtttaata tctacgaagg tggtatacta tgtagtaagt gtagtaaact ttttgataat
    20281 aatataaaat tagacttaac aactataagt cttatggaat atgtattaag aaatgatatt
    20341 ttaacatgta gtaaagctaa agtatcaaaa tatataacac atgaacttga gaatattcta
    20401 gacaagtatt tgaaagttta tgttgataat attaatttta aatcattaca cgtattgcaa
    20461 agtgtaaaaa ataataaggg agtggataat gatgagtagt aatataacta ttgaaatggt
    20521 tgattcagtc tttgaaagag ttccaggagc aacttatgca gaagcaaaag aagcattagt
    20581 aatgtgcaat ggagatgtaa ttgaggcagt aatatattta gaaaataaaa aaaatacttg
    20641 taattgtaaa gcaaaatctg ctaaagaaac tatggaagaa gtatttggca aagatggaga
    20701 aaatataaag tttcaattaa aagagcttct tagaaaaagt agtgtagtgc gaataatagt
    20761 tgaaaaagat ggaaaaacta tgatgaacat tcctttaaca gttggagttg ttggattagc
    20821 ttttgtacca ttagctactt tagttggatt atcagctgct gttataagta aatatcgtat
    20881 aaaagttcaa aatgaagaag atggagaaat tgtagattta ggtgaattga atgaagaaaa
    20941 acttcacgtt ttgaagaata tgataactaa tgcagcaaaa gatgtgaagg atgtagtagt
    21001 tgacaaaaaa gaagatgaca aagatgtaac tgctgacctt atgaaagaag aatctgaaaa
    21061 gaataaagaa gacgaagacg aaaataaata gatataaagt gacttttaaa aaatgaagag
    21121 aagataatta taagaattat cactaacatt gacaaatttt atagaatttg atattctaaa
    21181 agttgtagaa gaactattaa aatagccttt cgtgtagacg agaggcttta ttgttatagg
    21241 agggattttt atgaattttc aagagatgat actggcgttg caaaaatact ggtcaaagca
    21301 aggttgtatc atgatgcagc cttatgatat tgaaaaaggt gcaggaacta tgaatcctaa
    21361 tactttttta agatcattag gacctgagcc ttggcaagta tgctatgtag agccttcaag
    21421 aagaccagca gatggaagat atggtgaaaa cccaaacaga ctatatcaac atcatcaatt
    21481 ccaagttata ctaaaacctt ctccagataa tatacaagaa ttatatttgg aaagtttaaa
    21541 agagattgga attgacccaa gtgagcatga cataagattt gttgaagata actgggaagc
    21601 tgctacagtt ggtgcttggg gacttggatg ggaagtttgg ctagatggta tggaaataac
    21661 tcaatttacg tattttcaac aagtaggtaa tattgaatgt gagcttgaga caggagaaat
    21721 tacatatgga ttagaaagat tagctatgta cattcaagaa gttgatagtg tttatgattt
    21781 aaaatggaat gataaaataa cttatggtga agtatttaat aaagctgagt atgaaaactc
    21841 aatgtacgca tttgaattat gtgatgcaga tatgttgttt aatctgtttg atatatatga
    21901 aaaagaagca ttaagactta tggaaaatgg ccttgtaata ccttcatatg actatgtgtt
    21961 aaaatgctct catgcattta atacattgga tgcaagaggt gcaataggag ttagtcaaag
    22021 agcttcattt ataggtagag ttagaaatat ggcaaagact gttgcagaaa cttttgtaaa
    22081 acaaagagaa gaaatgggat tccctctttt aaaggacggt gacaaataat gaaaaattat
    22141 cttttatttg aaatcggtgt tgaagaaatg ccatcaagat ttgtaggcag tacactagaa
    22201 caattaaaaa acaatcttag taaattattt gatgaaaata gaatagcttt tgataaaata
    22261 aatgcgtatg ggacaccaag aagacttact ttagtagtcg aaggattgag tgatagacaa
    22321 aatgatttag aagaggaaat aaagggtcct gctaagaaaa ttgctgttga tgcagaaaat
    22381 aatttaacaa aaccagcatt aggatttata aaaagcaaag gattaagcga atctgacata
    22441 tattttaaac aagttggaaa agaagaatat atatttgcga ctataaagca agatggaaaa
    22501 gaaacttcag aagtactaaa agacatatta ccagagacta taaaatctgt agtattccca
    22561 aaagctatgc gttggggagg aaaaaatctt aaatttgcaa gacctataag atggatagtt
    22621 gcattgttaa atgataaagt attagagttt gatttagaag gaataatatc ttctaatgta
    22681 acaaaaggtc atagattttt aggtgaaagt acaatagaag ttgattctat agaagattat
    22741 tttgaaaaat tagaaaaaaa ttatatagtt ttagaccaaa acaagagaaa agaaatgata
    22801 agaaaacaat gtatagaagt ggcaaattct ttaggtggag aagttgaatt tgatgaagat
    22861 ttacttgaag aggtaactta cttagttgaa tatccaactg ctttttatgg agaatttgat
    22921 gtggattata taaaactacc aaaagaagtt gttataactc ctatgaaaca acatcaaaga
    22981 tattttccag tacttaaaga aggaaaactg ttacctaact ttatagctgt tagaaatggt
    23041 gattcttaca gaatagacaa tgttaaagct ggaaatgaaa aagttttaga agcaagactt
    23101 gcagatgctt tatttttcta tagagaagat actaagagaa acttagaaag ctacatagaa
    23161 aaattaaaaa ctgttgtatt ccaagtaaaa ctaggaacta tttacgacaa aactttaaga
    23221 cttgaaaaat taagtgctga tatacttgat agtttaggtt tagtagatga aaaggcagat
    23281 actataaggg ctgctaaatt atctaaggca gatttagtta ctggaatggt aggagaattt
    23341 gatgaattac aaggttttat gggaaaagaa tacgcaaaag ttggaggaga aaatgatgtt
    23401 gtaagtgaag ctatttttga acattatctt ccaagatttg caggagatat acttcctaag
    23461 actaatgcag gagttgcctt atcaatagct gataagttgg attcaatagc aggtttcttt
    23521 gctataggaa tacaaccaac aggttctcaa gacccatatg ctttaagaag acaagcttta
    23581 ggtatactta gtatacttat ggacagaaaa gttgctgtgg atataaaagt acttattgaa
    23641 catgctttag acaattattc tgaattagat tttaataaag aagaagttgt tgagcaaata
    23701 atgaatttct tcaatgagag agtaaaaaat ctatttaaag atttaggaat aagatatgat
    23761 gttgttgatg ctgttttaag ttctgatata aatgatgttt ctgatatgca tatgagagct
    23821 ttagagctta ataattggct agaaaaagat gaattagtgg agatgcttac tgcatttaat
    23881 agagtttcta ctctagctca aaaagcagaa agtgataaaa ttgatgaatc tttattaaaa
    23941 gaagatgctg aaattaaact ttacaatgaa tttaaacaag taaaggtaaa agttaatgaa
    24001 ttattaaagg ataagaaata tggtattgct ttagatgaat ttgcatcttt aagaccagta
    24061 gttgataatt tatttgatac agttatggtt atggataatg atgaagctat taagaacaat
    24121 agacttgctt tacttaaaca agtatatgat actatgctag gaatttgtga tttatctaaa
    24181 attgtttata aataaaattt aaactattta taagaaataa ggctgtctca aaatagagat
    24241 agtcttattt ttcattgttg agatgatgaa aatatatttg atttcataga aaaagagtgt
    24301 ctcataaact aaaaagttca ttttgagaca cccatgcttt ttgtaagtaa atataataaa
    24361 ttatatttta tacttatcag ttattttgtt taaaatatta tcatttgaat ttacccattc
    24421 ataaaaattg cctgatgatg cgtccgatat tttataaagc atttcaggaa tatcactagc
    24481 tttaaaatct ccaagtgatt ttcccaatct agtcttacct atttcaaaac tgcctccaac
    24541 aaataattca aatgcatctg tagatatacc atcaacattc ttctttttac cacataaacc
    24601 tatgctacca acttgatgaa caccacatga atttaaacat cctgatatat ataacttagg
    24661 tgctttattt aaaatttctt tattattttg taatctaaaa taatctatga tttcatgtaa
    24721 cattttctga ctattttgta tgcccatttg acaaattgga acacctatgc aagatatact
    24781 ttgttctaat tgagaattac aactaatagt tttacttatt tctaatattc tttttgcttc
    24841 atttccattt aaatttaaaa tatataatcc ctctgtcatt cctagtctaa tcataggatt
    24901 ttcaatatta tctaattctt taagtaaagt acttaaatcc tttgttaaca aaatacctcc
    24961 aactggatgt atatatacag tgtataaacc atcctgtttt tgaggtatta gtctagaatc
    25021 ctgtatatct attttaaccc caggtttaga gtaatctatt tcttcaatat tgagttcaag
    25081 actaccattt tctttttcta atttatagta ttctttaaat ttttctaaga ataatttttc
    25141 accaagtgat tcgaccatat atctaaccct agctctgttt ttattttcat aatttccata
    25201 atccataaag aatttaatca taccttcaac acaatataag gcatctttcg gttttataag
    25261 ttcatcaagt tctaaagcaa ctttgggttg tttacctaga cctccaccta agaaaactct
    25321 gaaatatggt tcatcatttt tcaaagtagc aacaaatcct aagtcttgta tactacaatg
    25381 agcagtatct gtgtaacaat tagaataaga aacttttaat tttctaggta aatggtatgt
    25441 agtagcattt ttgataaaat atttatcagt agcaactgca tatggagtaa catcaaagac
    25501 ttcactaggg tcaactccag ataatggaga taatccgaca tttctaggat aattaccacc
    25561 accacctcta gtgaatatat cattttttat tccttcttcc atgatatcaa caattgaatt
    25621 tatagataaa tcgtgtaatt gtatagcttg tctagttgtt aagtgtatct tatctaagtt
    25681 gtgtttaata gctatttcat agattttatt aagttgatac tgagataaaa ctccagcaga
    25741 tactctaagt cttatcataa aagatttttt gtctctttga gcataaacac catatccacc
    25801 agaaaatccc ttatattgca ttttatttat ttctccattt aaaaattttg aagaatattc
    25861 tttaaaatta ggtagttcat ttaacaatat ctttttaaaa ttatccatta tttcacctca
    25921 ttgttagtct tgtataaaaa tatatctatt gtataataga tatatatatt attatctagt
    25981 caggataaaa atataacaga ataattctat taaaagtaag aataataagg acatagtgag
    26041 tattttgttt ttattttaca aattaagtat aatatttttg ttttataaaa tataattaag
    26101 ataaatagat aattttgtaa ttttaatata taatatttag tagagtgtgg gagtagcact
    26161 taatatttga aggaggagtt tttgtatatg ggagaaataa taaatgcatt agatagagcc
    26221 ctagacataa ttttgctgtt atatcatgaa aagagagaaa tgggaataac tgaaataagc
    26281 aaggctatgg gagtatataa aagtactgta catagaactt tggttactct tgaaaacaaa
    26341 ggttttgtta tacaaaatgc agagaatggt aagtattggc taggaataaa tttatatgca
    26401 ataggtatgg ttgtgggaga aaaaatgtca ttgactgaaa tagtaaaacc atatacgaaa
    26461 aaattaaatc aagaatttaa cgaagtagtt aatgtatcta tattagaaga aagagctcaa
    26521 gattctccaa gaagtatcat aatacataaa gagtatggtt caaatcaatt actttcagta
    26581 aatccatccg ttggttcaag tagtgaatgt tattgttcag cagttggtaa atgtcttatg
    26641 gcatttaatg attctataga ttttgaaaaa tatagaaaaa caccaataca caagtataca
    26701 gaacatacaa ttgataattg ggatgatatg atgctttttt tagcaaaaat aaaagaacaa
    26761 ggatatgcta tagatgatga agaactagaa catggactaa cttgtattgg agcacctatc
    26821 ttagataaaa ataataaagc tattgcagcc ataagtttgt caggtccaac tataagaatg
    26881 agagaagggg actttgagta taagattaaa agagttatag aaactgcaaa aagtatatct
    26941 gaacttttta gatgattttt aatctcgata ataaaaatag gctatattta aattgttatt
    27001 tgtaaatatc agtttaaatg tagcctattt ttctttaatc taaaattaaa tattcttaaa
    27061 atttaaaatg ttattgaaat atataagcga atgtgttatc ttttaattaa gcaataaaaa
    27121 tagaacaagg ttctatacta tagaacaaaa ttgatgcata tatagtattt atacaaaata
    27181 taaatattat atatttaaaa ttaagtaaaa atattacata aaggggagat ttgagaaatg
    27241 gaaaaagtca agttgagtgt tgaaagatgt aagggatgct atctttgtat tgaagcttgt
    27301 aaaaaagatg caataagtct ttctgactac acaaataaaa aaggctttgt aactattaaa
    27361 gtcgatgaag aaaaatgtat tcaatgtggc gcttgttata caatgtgccc tgatttagta
    27421 tttgagatat tataaattat ttttattaga ggggagaaaa ggtatgcaaa gtttattaat
    27481 taaaggaaat gaagctttag cagaaggtgc tatacgtggt ggctgcagat tttatgcagg
    27541 ttatccaata acgccacaat cagaaatatt agaaatgatg gcatggagac aaaaagaagt
    27601 tggaggagta tttatccaag gtgaatcaga aatagccagt gtaaatatgg ttatgggagc
    27661 aagtgcactt ggagcaagag ctatgacttc ttcatctggt ccaggatttt cattaaaaca
    27721 agaaggtatt gcatattggg tatcagctgg tttgccagca gttgtagcat gtgtgcaaag
    27781 atatggtata ggtgatggat ttataggagc tggtcaagat aattattggc aagcagtaaa
    27841 aggcggtgga aatggagatt atcatttaat cgtgttagca cctaatagtg ttcaagaaaa
    27901 tatggatatg gcatatgaat cttttgaatt ggctgaaaaa tatatgcatc cagtacttat
    27961 actttctgat gctacaatag gtcaaatggt agaaccttgt atagcaccac caatgaaaga
    28021 atacgatatg gataaagctc catgggctgc aaaaggaaca cctgtaggag aaaaaaataa
    28081 agtaataact gatataacat attttgaaga ccaagataca tggcaaaaag ggtatataga
    28141 aaaaatgaga aaagttcaag aagatgaaca aagatgggaa gagatagaaa tagaagatgc
    28201 tgaagtggta tttgtggcat acggtatttc atcaagaata gcagaaggtg cagttagaga
    28261 tgctagagca aaaggaatga aattaggtct aataagacca aaaacattgt ggccttaccc
    28321 aagaaaagca tttaagaaat taagtgattc attgaaggga cttgtaacag ttgaaatgaa
    28381 catgatggca cagatgaagg aagatgtaat tatagcatgt gataatagat ttccagtata
    28441 ttcagtagca acagctcaat atatggcaga agtaaatcaa ttaatagatt ttgctcaaca
    28501 gattatagat ggaaaagcta agcaagagga ggtattttaa ttatggctaa aaaagcacca
    28561 gcaataatga tgtgtgaaaa tatgttctgt ggaggctgtg gacactctat agtcgataga
    28621 attattggag aagtattaac agaaatggat atatgtcaag atacaattat atgttcagat
    28681 ataggttgta atcatatgtt tcaatttagt atgaatgtag atgttgtcgt tccacctcat
    28741 ggtaaaatgg gagcagcaat ggcagctatg aaaagagtta gaccagataa atatatattt
    28801 acaatagctg gagatggtgg agcttatgca atagggcttg gagaaactat gtcagctgct
    28861 actagaaatg ataatgtaat atttcttgtt atgaacaata atatgtttgc aatgacagga
    28921 gggcaaatgg ctccaactac tatagaaggt caatatacag cttctactcc agcgggtaga
    28981 aattacgcag atcatggttc aacatttgat gttgtaaaag ttatgggaaa tttagatata
    29041 gcatatctag caagaggaac aataactaac cctaaagaaa taaatacaac aaaaagatac
    29101 atcaaaaaag ctctagaaaa acaaaaagaa aagaaaggtt tttgcttaat agaagtgctt
    29161 gtaccatgtc caacaaactg gggattaaag ccagtagatg ctatgaagag aattgaaaat
    29221 gaacttgttc caaagtatcc acttggagaa tttattgatt agggaggaaa taatatgttg
    29281 aatgaaatta tatgtgctgg ttttggagga cagggagttc ttacagcagg aaaaattata
    29341 atgtatgttg catataaggc agaaaagaaa attacatggt tcccatctta tggaaatgag
    29401 atgaggggag gaagtgcaaa ttgcaatgtt gttgtaagtg atgaaaaaat tgcaaatcct
    29461 tgtgtaaaaa atccagatat actattggga atgaacagcg tatcagtaga taagtatgaa
    29521 agtttaatga aaaaaaatgg ttatatgttt gtcaatgaat cactagtaag taaggataga
    29581 aagtacagag aagacattaa tgttataaaa gtacctgtta ctgaaatagc acaaaattta
    29641 ggcaatgaaa aagctgctaa catttgcatg ttaggtgcta tggtaaagag tttggattta
    29701 tttacaaaag aagaatttga aagtggtatg tgtgaatact ttgaaaatca aggcaaaggt
    29761 aaattcaatt ctaaaaatgt agaagcattt gaagctggat acgactacga atgaatattt
    29821 taaatattta aaataattat tgaaatactt atttttatat gttagtattt aattaataaa
    29881 atatagaacg tcgttctgga caatgggacg aagtctttag gtcggaatac aagaacggcg
    29941 ttctgtataa tagaactagg gggtgttaaa ttgaaagttt tagtaataaa tcctggtggt
    30001 acatccacaa aaatttctgt tttccaagat gaaaatgaaa ttttgaaaaa aaatataacc
    30061 catacaagag aagatttaaa aaatttttct aaagtatttg atgaatatga ttatagaaaa
    30121 caactgatag tagatatttt aagtagtgag aatcatagta taaattcttt tgacgcagta
    30181 gttggtcgtg gtggacttat gaaggctata aaaggtggta cctatactgt gagtgaagaa
    30241 atgattgagg atatgagaaa tgaaataaat ggggaacatg catcaaattt aggtgcatta
    30301 ttagctaaaa ctatagctga tgaaatagga gtacaatcat ttgtagtaga cccagtttct
    30361 gtagatgaat ttgatgatgt atcgaggatt acaggaatat cagacataga aaaagcaagt
    30421 tggttacatg cattaaacca taaagctgtt tcaagaaaaa ttgcacaaaa actaggggga
    30481 aaatacgagg attataactt tattgtagct catttaggtt caggaatatc gattgtagca
    30541 cataaacaag gcaaaatgat tgatggaagt ggtggtaggt ctgatggtcc attttctcca
    30601 gaaaggagtg gtggattact tgcatatcct cttatagaat tgtgttactc cggaaaatat
    30661 actcatgatg aaatggtcgc aaaagtaagt tcgattgggg gaatgtacga ttatttagga
    30721 acaaaggaca tgatagaaat tgaaaacaga atcaaggagg gtgatgaaaa ggcaaaactt
    30781 ataatggatg catttattta tcaagtagca aaagaaattt ctatgtatgg agcttcactt
    30841 tcagggaatg tagatagagt tatcttgaca ggagggatat ctcactcaga aatggtagtc
    30901 aaaggagtag cagaaaaagt ttcatatcta gctccaatag agatagtacc aggagagatg
    30961 gaaatggagg cacttgctct aggggcactt agagtactta atggagaaga aatagcaaaa
    31021 acttattaat atttaggaga aagagtatgt taaaagattt taataattta ttagaagagg
    31081 ctaaaaagct tgaacctgtt attgtaagtg ttgctgttgc agaagacaaa gaagtattaa
    31141 aggctgtgaa atcagcaaca gatttaaaca ttataaagcc aattcttgta ggagacaagg
    31201 ataaaataga aagtatatca agagaaattg gattaaaagg tcatgaaata ataggttgtc
    31261 aaaatcccga tgaatcagtt aaaaaagctg tagagatagt taaaaataag gaagctaaag
    31321 ttcttatgaa gggattagta aatacaagtg tctatatgag agctatatta aataaagaaa
    31381 cgggtcttag aacaggaaga ttattaagtt tgcttgcagt atatgaatta cctcaatatc
    31441 ataaacttct ttattgtact gatagtggaa taaatgtatc gccaaactta aatcaaaaaa
    31501 aagatataat gacaaatgca cttcttacta tgaaaagtat aggcatggaa aatcctaaaa
    31561 ctgcgatact tactgcaaat gaaatggtag accctaagat acaatctaca gtggatgcca
    31621 acctacttgt agagatggaa aaatcgggag aaataccaaa gtgtatagtg gaaggtccaa
    31681 tagcatttga tgtagcattt gatgcacatg cagcagaaca taaaggtatt aaaagtaata
    31741 ttgctggtga tgttgattta ttagtatttc caaatataga agcagggaat atattaggaa
    31801 agtcatggct tagatttaat aaagctaagt gggcaggtat agttttagga gcaacaaatc
    31861 cgataatttt aggttcacgt tctgacacag ctgaaataaa aataaattca atagcattag
    31921 cgtgcctagc atcacaataa gaaattatta ttaaggagga aaaagtatta tgaaatatgc
    31981 aaaaagaatg gatatggtaa aagcttcagc tataagagat tctcaaaaga aaatagctca
    32041 gaaggtagca agtggtggaa ctgttatatc ttttgctgca gggcttcctg acccaaattt
    32101 atttccaata gaagagataa gtgaaataac tcaaaaagta cttaatgaaa ggggaaaatt
    32161 tgccctagca tatggaccta ctaaaggtga agatgagttg ttagatctat tagtaaaaag
    32221 aatgaaagaa gaagaaaata ttgatacaaa agcagaaaat attataataa ctactgggtc
    32281 acaacaaggg atagcattaa gtgctatgat tttattagaa aagggagatg tagtagttac
    32341 agaaaatcca agttacttag gtgcaataaa tgcatttaga ccttatgaat gtgatttttt
    32401 aggagtagat acagatgaag aaggaatggt aacagatgat ttagacaaat tattatctga
    32461 aaattctaac ataaaggtga tatatgtaat accaacattt caaaacccta caggaaaaac
    32521 atggtcttta caaagaagaa aagattttat ggaagtcgtg aataaatatg atgtcatagt
    32581 aatagaggat aatccttatg gagaaataag atttacacca gaacctctac ctacacttaa
    32641 atctcttgat actaaagata aagtattgta ccttgggtca ttttcaaagg tattatgtcc
    32701 aggatttaga gttgcttgga tgtgtggtaa tgtagacatc atagataagg cagaactttt
    32761 aaaacaaggt atagatttac agtcaaatca attttcgcaa gtacaagtaa tagaattttt
    32821 aaagaaatac aacttaaatg aacatataga aaaaataaga gcagagtata agaaaagatg
    32881 tctgctaatg ttagatgcta tgaagaaaca tttcccagag gaagctaaat atacagaacc
    32941 agatggagga atgtttatat gggttgaact tcctgaaagt ataaatgtag atgatttatt
    33001 agataaagcc attgatgctg gagttgctta tgtttctgga gagtccttct ttgcaaataa
    33061 tggtccaaaa aatactatga gacttaacta tacaacaatg tcagaagaac aaatcgttaa
    33121 aggtgttgag atacttgcag aagttataaa aaaagaactc aattatgctt gtgaataaat
    33181 ataaaaagtg agggtttaaa tatgggagaa aaagaaaacg gtataaagtt tggaaagtta
    33241 atgtttcaac ttgttattat agcaatatct tgggaattag tttatattat accatttatc
    33301 caatatacat tatatgatcc aatcctaaaa gcattagaat gtacaaacac tcaactaggt
    33361 tttctactta caatttatgg gttaggtaat atatttggag ctcctatagg aggctggtta
    33421 gcagatagat ttgattatag aaagataatt ttaggttctg tatttcttaa tggagtagta
    33481 tcatttttat ttgcatttaa tatgaactat ccatttgcag ttgctacttg ggtaggttgt
    33541 gcaatcacat cattagttat gaactatcct tctatggtaa aaatacttag agttatagga
    33601 aaagataatc aaggtaaggt ttatggattt aatgaagcta tggttggagt ttctggagta
    33661 gtaatgggag caatattctt atatatttat acatgctttg caactcctac attgggtatg
    33721 agatgggtaa tgatatcttt aggaatatta tcaatagcaa tgtgtccagt attatggttt
    33781 gtaattaaag atgtagatat taaagaagaa agagaagaag caggtgaaaa aatgtctgct
    33841 ggtgatttta taacagtact aaaatcacca aatacttggc tagtaggaat aagtatattc
    33901 tgtgtatatt cattcacagt tacaatgtct tattttacac catatataac atctgtactt
    33961 ggaggttctg tagcattatc tggtgcatta gcaataataa gacagcatgg tctaaaatta
    34021 tttggagcac catttggagg atattgtgca gacaaattaa aatctcctac aaaagtactt
    34081 ttaccaatat atgtatttgg tatagcagta atagttttat tcctagtatt acctgcatca
    34141 actccaatga ctatattcat agctttaaca tttgtagtag gtatattagg ttacatggga
    34201 aaaggtatat attatgcagt acaagatgaa gtaaaagttc cagttaaata ttcagcaact
    34261 actataggta tagctgcagc tttaggattt tcaccagatg tattccaatt tgcattaata
    34321 ggacactgga tagatactta tggcaataaa ggttacacat atacatttat attccaaata
    34381 gcaattctag ttattggaat actatcttgt ttatatatat taaaggttaa aaagagaaga
    34441 gcaagtaaat tagaagctta aatattcaat ttggtaatta agtatatata aatacaatac
    34501 ttattttgat gaatgtgatt gtataaataa cataaaaaat atcaatagat taagttttat
    34561 aataatgttt agagaataaa gtataaaaat caaaatgtta tcaagttttc taggttttaa
    34621 cataaagtta cccctgaagg tgtaaaattg agtggcaatt attatctatt ttcttaccta
    34681 cataaaccta tttaatttaa attctaagag attttcaaaa tagatggtag ttgtactcaa
    34741 ttttaaacct cataataaat acgaaaattt aaaatctaac tagatactat ttaattatat
    34801 attaataaat atatattgta ctatttatat aataataatt tcaggctttt cattatatgt
    34861 at
//
LOCUS       DAYASV010000027        34752 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYASV010000027 DAYASV010000000
VERSION     DAYASV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.027"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(89..745)
                     /locus_tag="ACGI68_001352"
     CDS             complement(89..745)
                     /locus_tag="ACGI68_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0137797.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(800..1429)
                     /locus_tag="ACGI68_001353"
     CDS             complement(800..1429)
                     /locus_tag="ACGI68_001353"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0137798.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(1560..2201)
                     /locus_tag="ACGI68_001354"
     CDS             complement(1560..2201)
                     /locus_tag="ACGI68_001354"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFH0137799.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(2210..2881)
                     /gene="rpe"
                     /locus_tag="ACGI68_001355"
     CDS             complement(2210..2881)
                     /gene="rpe"
                     /locus_tag="ACGI68_001355"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0137800.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(2886..3770)
                     /gene="rsgA"
                     /locus_tag="ACGI68_001356"
     CDS             complement(2886..3770)
                     /gene="rsgA"
                     /locus_tag="ACGI68_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFH0137801.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDENEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(3868..4605)
                     /locus_tag="ACGI68_001357"
     CDS             complement(3868..4605)
                     /locus_tag="ACGI68_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0137802.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKADISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(4713..6728)
                     /gene="prkC"
                     /locus_tag="ACGI68_001358"
     CDS             complement(4713..6728)
                     /gene="prkC"
                     /locus_tag="ACGI68_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861606.1"
                     /note="PrkC, as found in Clostridium difficile, has a
                     kinase domain, two PASTA domains, and a C-terminal
                     Gly-rich repeat region; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing Ser/Thr kinase PrkC"
                     /protein_id="HFH0137803.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(6745..7497)
                     /locus_tag="ACGI68_001359"
     CDS             complement(6745..7497)
                     /locus_tag="ACGI68_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFH0137804.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(7517..8548)
                     /gene="rlmN"
                     /locus_tag="ACGI68_001360"
     CDS             complement(7517..8548)
                     /gene="rlmN"
                     /locus_tag="ACGI68_001360"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFH0137805.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(8556..9881)
                     /gene="rsmB"
                     /locus_tag="ACGI68_001361"
     CDS             complement(8556..9881)
                     /gene="rsmB"
                     /locus_tag="ACGI68_001361"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFH0137806.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(9943..10644)
                     /locus_tag="ACGI68_001362"
     CDS             complement(9943..10644)
                     /locus_tag="ACGI68_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFH0137807.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(10713..11465)
                     /locus_tag="ACGI68_001363"
     CDS             complement(10713..11465)
                     /locus_tag="ACGI68_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HFH0137808.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTAVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDIMDIEKAIGFLTGKNILVCN"
     gene            complement(11475..12404)
                     /gene="fmt"
                     /locus_tag="ACGI68_001364"
     CDS             complement(11475..12404)
                     /gene="fmt"
                     /locus_tag="ACGI68_001364"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFH0137809.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(12419..12859)
                     /gene="def"
                     /locus_tag="ACGI68_001365"
     CDS             complement(12419..12859)
                     /gene="def"
                     /locus_tag="ACGI68_001365"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFH0137810.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(12877..15366)
                     /gene="priA"
                     /locus_tag="ACGI68_001366"
     CDS             complement(12877..15366)
                     /gene="priA"
                     /locus_tag="ACGI68_001366"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFH0137811.1"
                     /translation="MKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVIKIGHRILVPFGK
                     GNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWMKNRYLCTYIECI
                     NLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVEKVIDSKGKIKVD
                     KLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESDKIDDYIEQNKIN
                     VGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLEFKDYYREPKSIY
                     KSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVYMEIIEFALNQGL
                     DSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAVKAGKVRVLIGAR
                     SALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNITLILGSATPSIE
                     EYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSLKLQKEIRYAIEE
                     NNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRCHYCGYEKEIPKE
                     CPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGALDEILNKFKDKEA
                     DVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLITQVSGRAGRADKE
                     GKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNMLSVVVSGEDERLV
                     IKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQILFKDENIEINLLK
                     GIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(15641..16840)
                     /gene="coaBC"
                     /locus_tag="ACGI68_001367"
     CDS             complement(15641..16840)
                     /gene="coaBC"
                     /locus_tag="ACGI68_001367"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFH0137812.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(16845..17111)
                     /gene="rpoZ"
                     /locus_tag="ACGI68_001368"
     CDS             complement(16845..17111)
                     /gene="rpoZ"
                     /locus_tag="ACGI68_001368"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFH0137813.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(17114..17731)
                     /gene="gmk"
                     /locus_tag="ACGI68_001369"
     CDS             complement(17114..17731)
                     /gene="gmk"
                     /locus_tag="ACGI68_001369"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFH0137814.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLKELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(17761..18642)
                     /locus_tag="ACGI68_001370"
     CDS             complement(17761..18642)
                     /locus_tag="ACGI68_001370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFH0137815.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(18646..19476)
                     /gene="dapF"
                     /locus_tag="ACGI68_001371"
     CDS             complement(18646..19476)
                     /gene="dapF"
                     /locus_tag="ACGI68_001371"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFH0137816.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(19763..20797)
                     /gene="ltaE"
                     /locus_tag="ACGI68_001372"
     CDS             complement(19763..20797)
                     /gene="ltaE"
                     /locus_tag="ACGI68_001372"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFH0137817.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            21093..22868
                     /locus_tag="ACGI68_001373"
     CDS             21093..22868
                     /locus_tag="ACGI68_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HFH0137818.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(23083..24735)
                     /gene="cplR"
                     /locus_tag="ACGI68_001374"
     CDS             complement(23083..24735)
                     /gene="cplR"
                     /locus_tag="ACGI68_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Contributes to intrinsic pleuromutilin, lincosamide
                     and streptogramin A resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein CplR"
                     /protein_id="HFH0137819.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     IQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(25034..26245)
                     /locus_tag="ACGI68_001375"
     CDS             complement(25034..26245)
                     /locus_tag="ACGI68_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0137820.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(26529..27062)
                     /gene="pyrR"
                     /locus_tag="ACGI68_001376"
     CDS             complement(26529..27062)
                     /gene="pyrR"
                     /locus_tag="ACGI68_001376"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFH0137821.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(27422..28369)
                     /locus_tag="ACGI68_001377"
     CDS             complement(27422..28369)
                     /locus_tag="ACGI68_001377"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFH0137822.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(28338..28784)
                     /gene="lspA"
                     /locus_tag="ACGI68_001378"
     CDS             complement(28338..28784)
                     /gene="lspA"
                     /locus_tag="ACGI68_001378"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFH0137823.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLVDR
                     VRLGFVVDYFDFRVIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(29094..29873)
                     /locus_tag="ACGI68_001379"
     CDS             complement(29094..29873)
                     /locus_tag="ACGI68_001379"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0137824.1"
                     /translation="MKKIICGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(29928..30410)
                     /locus_tag="ACGI68_001380"
     CDS             complement(29928..30410)
                     /locus_tag="ACGI68_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFH0137825.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(30676..32097)
                     /locus_tag="ACGI68_001381"
     CDS             complement(30676..32097)
                     /locus_tag="ACGI68_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HFH0137826.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(32243..32422)
                     /locus_tag="ACGI68_001382"
                     /pseudo
     CDS             complement(32243..32422)
                     /locus_tag="ACGI68_001382"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(32435..33013)
                     /locus_tag="ACGI68_001383"
     CDS             complement(32435..33013)
                     /locus_tag="ACGI68_001383"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137827.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGI"
     gene            complement(33018..34220)
                     /locus_tag="ACGI68_001384"
     CDS             complement(33018..34220)
                     /locus_tag="ACGI68_001384"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0137828.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        11060 a         6384 c         3485 g        13823 t
ORIGIN      
        1 caataaaacc tactttttaa ttttttaaat atttattttt aatttatttt gtttttaatt
       61 tacttcgatt ttaaaatgtc cttgactttt attttataaa tgtacatatt tcttctactg
      121 gttttttaga tggactcttc aaattgtcct ctggcactcc aagagctaac attgcaccta
      181 aaaaatactc tcccttttca aaaccagttt ctgcttctaa aacagcttct atctcatctg
      241 ctgcatagtt ttgacttgtc aaccaacaag aaccatatcc aagttcaatt gctgatagtg
      301 taaagttttc aattgcagca cctagactct gcatacctgg atttcttata aataatttat
      361 ctatagtctc cttaggtgca tctataagct ctaattcata atatcctgat ggattatata
      421 cctttgtaaa aactaatact aaaactggag cttttaagta aaatagtgta aaatttttaa
      481 caaattttct aaatctatta gccttatcca ctgatacttt atccatttct acaagtattt
      541 cttgttgctt tttagttatc acatctgcta tcttttccat taaatcacgg cgttttataa
      601 caacaaaatg ccagttttga atattcttgc ctgatggtgc tgcacctgct gcttttatca
      661 tttttaaaat atcttcatct gatacatctt gatttttaaa ctttcttaca ctttgtcttt
      721 taaaaatagt atcttgtaat tccattttta aatcccccca taactttttt attattttaa
      781 tataattttt aaactcatct tattttaata tatcatgttc taacaaatct ttaaatgaat
      841 ctataaaata atcatataca tctacattcc caaacccata tttggcatat ataaaaggta
      901 tacctacaaa cttagcacta tttgcatcac cttgcgtatc accaacatag attggattta
      961 ttaatttatt tctttcaatt ataagtttat tattttctcc tttatgcaaa ccagtattac
     1021 caggacattc aaaatctata aaatactgtt ctagtttatg tgcttttaaa aaagattcta
     1081 tatatccagc ttgacaatta cttactataa ataatttata ttttttagat agtaatttaa
     1141 gagtatcttc tatatcatca aataatttag ctccattttc agataaatag tcacattctt
     1201 tcaaagaaca ttcattcaat aaattcattc tttcttcttc acttaaattt ggaaataaac
     1261 gatttgctat ttcctcaaaa gaaagcccca taagacttct aaataaatct tttgtgacac
     1321 ttaattctat ttccttatgc tcacttaaaa catcctgcca gactttgcat acttcttcag
     1381 ttgaatccca caatgttcca tctaaatcaa aaataatact atctaccaca aaaaatcccc
     1441 cttatgtcaa agtttattat atatttataa ttatatacta aaaagcagat acaatcataa
     1501 atatatttta cttgattgta tctgctctac aatataaaac caattgtcaa aatcaaattt
     1561 tataaatttc taactacaag tacacagcct tgctctactt taatcttaca agaattactc
     1621 aacataacat ttgatattcc aataggtaca gagtatttca tataataatt atcgagaggg
     1681 tattctaagc cagtaagtgt tacaccctta gcatcaccat ttatagctat tacagaaata
     1741 gtgtcccctg ggtttccata caatgaaatt tcctcatttt caactatata catctcttct
     1801 ttatcagaaa gtatcctaga atatatacca tcttctttta aataatataa tagctttata
     1861 tttgcaagag tgtggtcaat ccttccccct aaagcaccaa aaaaatcaat atgatttgct
     1921 ttaagtgtct tagctaaaaa aagacaaagc tctgtatcag tctcatcttt tttagatgga
     1981 aatttttcaa atttaacacc tttattttta tagaagttta tcttttcttc ttctactgaa
     2041 tctaaatctc ctaatatata atctggcatt atttccattt tataagtgtg atttgcccct
     2101 ccatctgcac atattatgca gtcataacat tccttaatta taatatctct tgtaacttcg
     2161 taatcttcaa tttcgccatt taaaacaata cagattttca ttattaaatt taaactaatt
     2221 cttcaagaga agcattcttt ctaaataaat taactgtttc ttgaatgtca tcacttccaa
     2281 atattgcaga acctgctact attacattag ctcctgcttg tacaacttcg gctacgtttt
     2341 tagggcttat accaccatca acttctatat caatgtttaa tccttgacta tctataatag
     2401 cttttaactc tttaatctta tctaaaacac aaggaataaa tgattgtcct ccaaatccag
     2461 ggttaacaga cattaataat accatatcta aatcaggtag tacatgttta atcgtatcta
     2521 ctggtgtagc tggattaagt actacaccag cttttattcc atgtgatttt atattttgta
     2581 tagttctatg tagatgaaca caagcctcct ggtgaacagt tattatatca catcctgctt
     2641 ttacaaattc ttcaatatac tggtctggat tttctatcat aagatgagca tcaaatacca
     2701 tatttatatt ttctttcttt aaacttttga ctacaagtgg tcctaatgta atgtttggta
     2761 caaagtgtcc atccattaca tcgatatgaa gatattcaca tcctgcactt tctacttttc
     2821 ttacatcttc taataatttt gcaaaatctg ctgataatat tgatggtgct aatttaatca
     2881 tgtttctaat acctcctact attattttgt cttatttcat gtagcagctg tatataacta
     2941 tcatatcttt ctttagatat ttctccattt gtaacagctt cttttatagc acaattaggt
     3001 tcattttcat gaatacactt tgaaccaaac ttacaatcat taaatttatc aaattctata
     3061 aaataatctt taagttcaac ttcctctata tcttcaaggg caagggaact aaaacctggt
     3121 gtatctgcaa ccattccacc aaattctaat tttaaaagtt cagcgtgacg agtagtatgt
     3181 tttccccttt ttattttatc acttacaact cctgtttgaa gtttaaaatt ttcatctatt
     3241 tcatttaata agcttgattt tccaactcca gatggtcctg caaacactac tacattttct
     3301 tttaattctt cttttacttt atctatattt aacttagtaa tattgcttac aggaatgact
     3361 ttatagccac taagttcata tatattttta acagtttcta atgtatcatt atcatctaaa
     3421 tctgcttttg taaggattat tacagtttct aaattttctt tttctgccaa aactataaat
     3481 ctatctaaaa gagataagtt tggtttagga ttttttattg cgaatactat taatgcttta
     3541 tctacatttg caataggagg tcttataagt tctgtatctc tactgtcgat ttcttctaag
     3601 ataccctttt tttcattttc atcaacaatg cttattttga ctctatcacc aaccaaaggt
     3661 gttattttct gctttctaaa tatccctcta gccttacatt cataaatacc attataagta
     3721 tctacataat aaaatccact aatccccttt ataatttttc cttctagcat caaatgcctc
     3781 cctttataaa atttatataa acacatactg tttatgagta gtatatcgtg ttattctttc
     3841 ctatttttat ataataccac atttttttta aataacaaat cctccgtttg gactaattac
     3901 ctgaccagtg ataaaatttg atttgtctga tgccaagaaa atagcacaat ttgctatatc
     3961 ttctggagtt cctagcctca ttagaggggt ttcttccaca agagcgtcta tatcttcctc
     4021 attatatcca gataacatgt cagtattaat gacacctgga gctatgctat ttactctaat
     4081 atttgatggt cctacttctt tagctaatgc ctttgtcata cctattatgc ctgcttttgt
     4141 tatagaataa tgcacttcac aagacgcacc tgatataccc cagattgaag atatattaat
     4201 tatatttcct tttttttctg atatcatgta ttttaaagca acttgtgaac aataaaagct
     4261 tccttttaaa ttaatattca tcatattatc ccaatcttca tcagttatat ctgtaaacaa
     4321 cttatcttga cttacaccag cattgtttac aagtacatcc aatcctccaa attcttttat
     4381 acaataatct accatatcct ctacatcttc tctatttgaa atatcagcct taaaaagttt
     4441 tacagaaaaa tttttttcat ttaatatagt gtacaactct ttagcttcat tttctgactt
     4501 attaaaattt actaatacat tgtaaccttc ttttgcaaaa gctttagaca tagctttacc
     4561 aatccctcta gctcctcctg taattaaaac tgttttcttc ttcataaata cttttctcct
     4621 tatattataa ccaattcagt attttataca aaacaaactg ccctttaaag aaaatatttt
     4681 ctataaaagg acagctctta ttttattaat atttattctg ttttaccaat gtttgcgcca
     4741 gttcctgatg aagttcctcc attatctcct gagtttgagc ctcctgaact atctccattg
     4801 tctcctgagt ttgaacctcc tgaacttcct ccattgtctc ctgagtttga gcctcctgaa
     4861 cttcctccat tatctcctga gtttgagcct cctgagtttc caccactatt tcctgagttt
     4921 gaacctcctg agttacttcc attattgcct gagtttccac cactatttcc tgaatttgag
     4981 cctcctgaat ttccaccact gtttcctgag tttgaaccag gttctgttgg tgtattattc
     5041 tctggtgttg ttggtttttt atctggtact gttggagtct gtgtatttga tttttctgaa
     5101 cctttgctta catataggtt tacctcatca ccttcctgag ctcttgatga gccagcaccc
     5161 ggactctgac ttaaaactgt accttcttta tatattgagc tgtattcata cttaacagtt
     5221 cccactttta agttattttc ttcaataagt tgttttgcat tactcaaagt aagtcccact
     5281 acattaggaa cagatgcctg acttgaacct ttacttacga caacatttat agtatcaccc
     5341 tttttgacat ttgtacttcc accttcaggt gtctgtgata ttatacaatt ttcatcaact
     5401 tcactattat actcttcact ttttacactc aggtataatc cttctttttc aacagcactt
     5461 tgagcttctt ctaatgtcat atttacaaga ttaggaacag tcaaagattt attgccaaac
     5521 cctccagcaa ataaaaactt atatgctaag aatacttgag cacacaatat cagtatcaaa
     5581 acagccgcaa ttacttttaa tcttcttcta gattttgggc tttcctgttt tttcttcttt
     5641 ttcttagttc tcttacttgg agtactctta ggtctctgat tctttttagc tctcattatc
     5701 tcttcctctt cttcatcttc atcatcatct tcttcataaa aatcatcata gtaatcttca
     5761 tcatcatcca aatcagctac ttcaacaggc ttaaccactt tttcaggagc tggttttgca
     5821 agtgttggat ttacaacttt attaatttct ttttcatcaa ttttcttagt tgcaaaatca
     5881 tcatattctt ttataaaatc taaatcaata tttttttcta tatactctat atcttctatc
     5941 aattcttcag cagtttgata tctatctgca ctagattttt ctgtaagctt ttttattgta
     6001 gttcttacac tttgaggaat cctaactttt tcttctgatg taaagtctat atcatcatta
     6061 atgtgttgaa gtgctataga aattggacta tctcccctaa atggaacttt tcctatcaac
     6121 atttcatata aaactatacc tagagaatac aggtcagcat tattagttac aaatttacct
     6181 ttagcttgct ctggtgaaaa ataatgcact gaacctatta tactaccaat attagtcatt
     6241 gttgaatttg aaacagcttt ggctatacca aaatcagcca cttttactac tcttccttca
     6301 tttgatatga gaatattatg aggttttata tctctatgta taatcccctt tttgtgcgct
     6361 gcactaagtg ccatagctat ttgttttgtt atatctaatg cagtatattc atctaatata
     6421 ccttcatctt gtataatctc ttttagattc ttcccatcta caaactccat tacaatgtag
     6481 tgaacttttc catcttcacc aacatcataa acatttacta tatttggatg tgaaagactt
     6541 gctactgctt ctgcctctct tttaaacttt gttagaaatt catcatcatc tacaaattca
     6601 ggtctaagta ctttaagtgc aacagtcctg tttaataatc tgtctttagc ctcatataca
     6661 aaggccattc ctccatcacc aatcttcctg atgatttcat atcgatttcc taaaattgta
     6721 tctcccacaa tatcaccgtc ctaattatac ttttatcact ataaccgaaa cattatcttt
     6781 accagaaaca tcattggcca tacttatcaa ttcttcactt atattactag ttctctcata
     6841 ttcataatct aatattaaat ctcttatatc ttcatcatca ataaacccag ttaatccatc
     6901 tgtcgcaaga agtattatat cacttttttt gatttctttc ttaaaaatat ctacaacaac
     6961 catatcatct gttccaattg ctctagttat tctattcctc tgtggatgtc ttcttgcctc
     7021 ttcttctgta atgacatggg ccatcataag ttcttcaacc acggaatggt ctatagttat
     7081 tttatcaaat ttttcatctg ttaacaaata acatctgcta tctccaacat ttgccacata
     7141 taagacatta ttatacacta tagccgtaac taatgtagtt cccattcctt caaattctat
     7201 atcttccata gattttttgt gcacaattga gtttacatta ttataagcct gttttaatat
     7261 atcatctata tagtctattt ttacattatc atgttgtaat aagttttctt ttagaaaatc
     7321 aattatattt tctactgcta atttactggc gacttcacct tttttatgtc cacccattcc
     7381 atcagctatg gcaaatattc ctattggacc atgttctgta tctataactt ctccctcaca
     7441 atagtcttca ttgttttttc ttatttttcc tatatgggag gcacaactat aaaccatacg
     7501 acttcctcct tacaaattac ttgtattttc ttctcaattg cccacaagct ccactaatat
     7561 cagaacccat agacatccta actgtggcag gtatattgtt cttctccaaa ctatccctaa
     7621 atttataaat aaatgcttta tctggttttt cgtattctct ttcttcaact ttatttattg
     7681 gaattaaatt tacatgacat agcatacctt ttaggagctt agctaatgcc tttgcttctt
     7741 tttctgagtc atttactcct tttataagag aatattcaaa tgtaaccctt ctatttgtct
     7801 tttttatata atatctacat gcattcaata tttcttcaat agaataagca tttgctacag
     7861 gcataatttt tcttcgttct tcatcatatg gtgaatgaag tgataaagct aaatttatag
     7921 ctatttctaa gtctgccaac tcatacattt taggtactat tccacaagtt gaaagagtta
     7981 tatgtctata ccctatatta agaccatttt tttcgttaat tatttttaaa aattgttttg
     8041 tattttcaaa gttatcaagt ggctcaccac tccccatcaa tacaagattt gataccctct
     8101 taccagtatc ttcttgaatt ttaataactt ggtctaaaat ttcccatggc tctaagtttc
     8161 taattaatcc atccattgta gatgcacaaa aattacatcc catcctacaa cctacttggt
     8221 tagaaacaca gacagtaact ctactatcat aatccatcat cacagtttct attatatttc
     8281 catcatctaa taaaaacaaa tatttttttg tattatctac cttagattct aactttaatt
     8341 ctatatctat atgccctata catgaaacct cttctaattt atttctcaaa ctttttggta
     8401 tattgttcat atcatcaaaa gtttttgcac ccttatatat ccaagagaat atttgacttc
     8461 ctctaaaggg cttttcatct atagttttca taaactcttt aagttcatct tcagtaaaat
     8521 tttttaatac tatctttttc ttttccattc tctcactacc ttattctctt taattttgct
     8581 atgaaaaaac catctatacc atgaatatta ggatatatct ttaaatatcc tttatcttga
     8641 ttgtctaagt ctacatttac tgtatcaatt ggaacaaatt caaagttgtg attttcttca
     8701 acaaaagaag taactatatt tatattctcc acatcttgaa ttgtacatgt actatatact
     8761 aaagttccac caactttgac atatttagat gcattttcta aaattttctt ttgtatagaa
     8821 gtgatgtctt ttaactcttc ttctttctta tacttaattt ctggtttacg tcttattata
     8881 ccaaaaccag agcaaggaac atctgctaaa acataatcaa atttacttat actattttca
     8941 tcaatctcag aagcatcaaa tctttctaca aacacatttt taagccccaa tctatttaca
     9001 gtagagttta taagctttag tttatgctca aaaatatctc tagctactac ttgaccagta
     9061 ttgttcatta aagtagctaa gtgagtagat ttccctccag gagcactaca tacatccaaa
     9121 acaaatgaac cttcttttgg attaattact ttccctacta tcatagaact tatatcttga
     9181 attgtaaata aaccagcctt aaataattca tttttttcta tatttttcat tttttcgact
     9241 ttaatagctt cttctaccat aggaactttt aaacatacaa tacccatttc acttaatttt
     9301 tcaataagtt cttctctact tattttaagt gtattcgttc ttatatagat actaggcttt
     9361 tcattatttg cttccaacaa atcttcagca aattcttgtc caaatttatc tatccagttt
     9421 tttataatcc aagagttata ggaatacttt atagataagt attttacact atcttcctca
     9481 gttatgtcca ttatagtctc tttagctctt atttcatttc taagaatagc atttatgaaa
     9541 ccagaagatt ttttatcata tttcttagat aattttacag tttcattaac tgctgcataa
     9601 tctgatatac tatctaaaaa taaaatctga tatgttccca ttcttaaaaa gattttaaca
     9661 taagttgaca ttttttttac ctttattttt gaaagtttat tgattatata atctaaataa
     9721 tatttatttt ctattactcc ataaattaac tctgttgcaa gtcctctatc catatcactt
     9781 atttctaaat ttttaaaatg cttatttatt gcaatatttg aataattgtc atttttttct
     9841 atatcacata atactttaaa tccaacttct cttgcatcca tataaatttc tcccttttat
     9901 taaacataaa aaagggttgt ttctaaacaa cccactttac ttttagtcat ttcttctttg
     9961 agctattagt aacaatctaa gtaattgaag tattgaaacc aatgctgctg caacatatgt
    10021 taaagctgct gctgaaagta ctcttctact ttgtcttgac tcactttcat ctataatccc
    10081 taaattccca agttgaacga ttgctctact tgaagcatta aattctacag gcaatgttat
    10141 tatttggaat aacactgaag ccgaaaacaa tagtatacca atttttaaaa atggaccagc
    10201 cataataaat cctaaaaata ttaaaaacca agatatactt gaagcaaaat tcactactgg
    10261 tactaaacta cttcttattt gaagaggtgc ataaccttta gcatgttgta tggcatgacc
    10321 acactcatgt gcagctactg caacagaagt tatagatgtt ccataatata catcttctga
    10381 caaccttaca gcctttcttc ttgggtcata atggtcactt agatgccctc taaccatttc
    10441 tatctttaca tcatacaaac catttgaatc aagtactctt cttgctgttt gctctccagt
    10501 atatcctcga cgtgtattta ctcttaaata cttatttgtt gttgaactca ctttaaattg
    10561 tgcatatata gtaagtaata tagctggaat taatacaacc attgttgggt caaatcccca
    10621 aaaaccgcca tatacagggt acataaataa cactctccta aaatatattt ttattataca
    10681 tcctcttaat attcacttca cttaaaaaca gtttaattac aaactaatat attttttcct
    10741 gttaaaaagc ctatagcttt ttcaatatcc ataatgtcta cattagtatt tacacaaggt
    10801 ccattaggtc ttttgttaaa tacacctaaa acaggaattt tcttaatatc ttgtattcca
    10861 gatgtcaaat ctctttcaca tgcaacagct acaacagctt taggtttatt atccattata
    10921 atttttctag ctagggttcc tccagtagct acaaatattt ttacattagt tttttccttt
    10981 agcttaatta attctcctat gttgcatctt ccacattctt tacaattatc tatatcatta
    11041 gtaactttta atttacaact attctcttga atacaatgag gtattaaaat tattatatcc
    11101 tctggattaa aattatattt attactgtat atatattcat tatttaactt tatatatatt
    11161 cttctaatat cactttttga cataccaaat gatgatgcaa tgaaagatat aataggaaat
    11221 aatccactga caatattaaa atttattttc attatatttg agttaacata ctttccttta
    11281 attactctat aagtaactgc tgttgaacat agtattataa gtattagtga tattattata
    11341 atatttatca tcaaggagaa tatttgagtt aaacttatta taaaaaaatt aacaactcct
    11401 atcaaaattc ctaatattaa aatcaaaatc ccaacacttg ttaaatactt ctttatatct
    11461 gtcatacttt tcacctaacc caatactaaa ccagtttcta tagtattccc ttttatatat
    11521 tcaccaacca acattctctt tttattcggc atctgaattt cttcaattaa aagtacatta
    11581 tctatagtac ttacttttat cccatctttg cttacatcaa ttattgtacc tacatctttt
    11641 gtgctttttt catctaacac tttagttttc caaactttca ttatgacatc attataggta
    11701 gtataggcac ttggccatgg atttactcct ctaactaaat tgtgtatttc tctggcactt
    11761 tttgaaaaat ctatgttacc taatgattta ttcattattg gcgcatagga aaattcttca
    11821 tgattttgta cttctcttgg agcatttcct tcttcaatta atcttaatgt ttcttttaaa
    11881 gtttctgcgc ctatattcat cattttatca tgaagttctc ctgctgttat attttcatca
    11941 agatttactt ctgtctttag tatcatatct ccagtatcta gcccttcatc catatacata
    12001 gttgtaacac cagtcttttc ttcaccattt attattaccc aatttatagg tgccgcacct
    12061 ctatattttg gaagtaaaga aacatgaaca tttatacatc caagtttagg aatctctaat
    12121 atttcttttg gaagtatctg tccaaaagct acaactacta ttacatctgg atttaaagat
    12181 ttaattttat ctataaattc tttatctcta gcctttactg gttgatatac aggtatatta
    12241 ttttcaattg ccagttcttt tactggactc atacctagtt ttttacctct tccttttggt
    12301 ttatctggtt gagttacaac acccaatatt tcatacttct catctattat tttttgtaaa
    12361 catggaactg ctatatcagg agttcccata aatactattt tcatcaaatc aactcctact
    12421 acttttctat tttgtcaaca aacaaaattc catctaagtg gtctatctca tgacaaaatg
    12481 ctctagctaa aagttcttct ccctctaact ctattgtttc accatttcta tttaaagctc
    12541 tgacctttac ataatttggt cttctaacat ttcccgcttc tccaacaaca cttaaacatc
    12601 cttcctcatc tatttgctcc ccagatgttt ctattatttc aggatttata agttctataa
    12661 gctcttctcc aatatctatt actactactc tttttaatat tccaacttga ggtgcagcca
    12721 gtccaacacc atctgcatca tacatagttt ctgccatatc atctaataat tgtattatct
    12781 tttcatcaat tttttcaacc ttttttgatt tttttcttaa tactggttct cctatttgaa
    12841 ctatttgtct taaagccatt ataattcctc ctaaatttat aatacactgt ttggattaat
    12901 atctatagat acattgatat tcttatcaaa tatcaaatct cgttttgtta tacatatata
    12961 ttttattata ccctttaaga gattaatttc aatattttca tctttaaaga gtatttgcca
    13021 tctataattt tgatttattt ttgatattga acatggattt ggacctaata taaatcctaa
    13081 atcatttata cccctttttt ctaataaata aatcaatgat gcatgcatat tttttatatt
    13141 ttttatcact agtctttcat cttcaccact aacaactaca cttaacatat tattaaaagg
    13201 agaatatcca aatgcttttc ttatttttat ctcatcttca taaaaaccct cataatcata
    13261 ttcaatagca tgcttaatag catagtgttc agtgtcataa gtctgaagta ctacttttcc
    13321 ctctttatca gctctccctg ctcttccaga gacttgagtt atcaattgaa atgtagtttc
    13381 aaaactttta aagtctggaa aatttaatat catgtctgca gacaaaatac ctactaaagt
    13441 tacattctca aaatccagcc ctttacttaa catctgtgtt ccaataagta catctgcttc
    13501 tttatcttta aatttattta gtatctcatc taaagcacct ttttttgaag ttgtatcttt
    13561 atccattctc aaagttttta tatctggaaa aatatatttt aattcctctt caattttttg
    13621 agttccaact ccaaatggct taacataagt acttttacat tcaggacact cttttggaat
    13681 ttctttctca tatccacaat agtggcatct tccagtattg cttttcttat gataagttaa
    13741 agatatatca caattttcac attgaaacac atatccacac tttctacaag atacaaaatt
    13801 tgcataacct cttctgttta aaaatagtat tacttgatta ttttcttcaa tcgcataacg
    13861 tatttctttt tgtaatttca aactaaatat acttctattt cctttatcta actcatcttt
    13921 catgtctacc acttcaatgt ttggaagagg cttatcattt gctctacttt ttatatttat
    13981 aagtttgtat tctccagatt ttgccctata atactcttca attgagggcg ttgctgaacc
    14041 taaaataaga gttatattat tttttaatgc cataaatcta gcaacttcta tagcattaaa
    14101 ttttggattc ttctcagact tatatgatga ctcatgacat tcatcaatta ttattaagcc
    14161 taaggaatta aaaggtgcaa ataaagctga cctagctcca attaacactc ttactttacc
    14221 tgctttgaca gctttgtaaa catcatgttt ttctccttca gatagtttac tgtggaacac
    14281 tccaaccaaa tctccaaacc tactctttag cctatctatt gtttgaggag ttaatgctat
    14341 ctcaggtact aagaatatac tgtctaagcc ttgatttaga gcaaattcta ttatttccat
    14401 gtaaacttca gtttttccac ttccagttac tccatgaatt aagtatggtt ttttatcatc
    14461 tacaaacata ttcgacttta tctcatcaac tgcttcttgt tgctcattgt ttaattttat
    14521 actttttagt acggatttgt aaatactttt tggctctcta taataatcct taaattctag
    14581 ggttattagc tttttcttac ttagagaatt tatactttgt tttgaagcat ttaataaatc
    14641 caataaatca tttatctcta cattttcatt atttttaaga aaacttaata tctctttttg
    14701 tttacttccc acatttattt tattttgctc tatataatcg tcaatcttat cactttctaa
    14761 actcaaagat atataacata ccttcttttc atttttgtga tttttatatt cccaacataa
    14821 ttttataatt ccattcttat tcattgtgta caaagaacta tttaagtttg gtatatattt
    14881 taacttatca accttaattt tacctttact atcaatcact ttctctacta tttctctatt
    14941 tttatcactt aaagttgata ttttttcctt caattcaata tcatttaatc cacttaaact
    15001 ttcacctaat aggactactt tataattatt taatttgtat ccctttggat atatcaaatt
    15061 tatacattct atataagtac acaaatatct atttttcatc caataaacca actctaaatc
    15121 ttcttttcta aatatcggat tttcatctaa tatatcaata atttctttta tttttatttt
    15181 ttcatctaag ctatctgtaa attgaaatac aaaggcttct gttggtttat taccttttcc
    15241 aaatggtact aatattctgt gcccaatttt tattacatca gataaaaata caggaatttg
    15301 atatgtaaat aaattatctg tatatatagt attacttcta actattacct ttgcatattt
    15361 tttcattata aaacctctac ttaaatttaa tatattgttc atattttaat atactttaca
    15421 agatattaaa atctcagaat ttataagcaa tttcaagtta ttaatattaa ctttatttta
    15481 ataattaact attaattata ttttatactt tatgacaaaa taaatgctga aaactacaat
    15541 ttaactgaca tttgcgagtt tttactcgct aaaattaaat tgcagctttc agccacttta
    15601 gctgtgataa aatacacaaa tatcatattt aaaaaaggtc ttatttctca agcaacgctt
    15661 taactttatc taaaataaca ttggctactt cttctttttt catcttagga tactcagtaa
    15721 tatttccctc tacatctatt atttttacaa tattagtatc agctctaaaa ccagcacctt
    15781 ctttagttaa atcatttgcc acaataaaat ctaaattttt cttttttatt tttcctttgg
    15841 catgttctat aaggtcatta gtttcagctg caaatccaat tagtatctta tcttctttta
    15901 tttttcctat ttcataagca atatcataat ttctacttag ttctattgat aaatcatcat
    15961 tcttcttttt aatttttttg tcagaataag tttttggttt ataatctgca actgctgcac
    16021 tttgaattac tattttattt tcacttaaat ttgatactac tgcttcatac aaatcttgtg
    16081 ctgactctat ttgaacaaat tttttaagat tttgtggaat agaaagattt gtaggacctg
    16141 atattaatgt tacatcagca cctctagcta tagcttctct tgctatagaa tatcccattt
    16201 taccagaaga cctattagtt aaatatctta ctgggtctat actctctaca gttggaccag
    16261 ctgtaactat catactttga ccttttaaat cttgctcttt agtaagatta gtcaaaactt
    16321 cctctactat tatatctgga ttagctagtt tacctgttcc aatatctcca catgcaagcc
    16381 ttccactttc cggttcaata aatttatatc caaagctctt taaagtttgt atattttttt
    16441 gaacaattgg attttcatac atattagtat tcatagctgg tgctataaga acctgtgctt
    16501 ttgttgccat aacagtagtt gtcaacatat cgtcagctat accatttgca atcttgccta
    16561 taacatttgc acttgctggc actattaaga ataaatctgc tttttttgct aatgatatat
    16621 gttcaacttc ccaagttttt ggttcttcaa acatatcttc tactacataa ttttgactca
    16681 gcgattgaaa tgtaagtggt ttaacaaatt ctgttgctga ttttgtcatt ataacatgga
    16741 tatttgcatt tagttttttt aacttactta caacatcaca agccttatat gcagctatac
    16801 cgccacttat gccaattaca acagttttat cttttagcat gaccttactc ctcttctgaa
    16861 atttgttggt gttgctcagc atgatgtcta gcttcttcta tctctatttc ctcttctgtc
    16921 aataatctat atgttatttt tccacttgca acttctttag ttgctacaca tacaggcttt
    16981 tccttagttt tatttgaaac atatggttct tctccatcaa ttaattttct tgctctcttt
    17041 gaaacagtcc ctactaaata gtatctgttg tctatttttt ctagtacctc attgatagat
    17101 ggttttaaca taattataat tcctccttaa atttctctat tataatattt ttatatctgc
    17161 taactttatt cttttcagat gatattatac cttctatttc tttagcagat gtttttacat
    17221 cttcattaaa tataaaataa tcataatcct taatcatttt aatttcttca tatgcacagc
    17281 taaatctctt ctctatttca gcttctgttt ctgtacctct tccaacaatt ctatttttta
    17341 attctttaag tgatggtggt agtacaaata taaatactcc ctctgggcaa acttctttta
    17401 tttgttttgc tccttgcatt tcaatctcca gtaatacatc ttgtcctttt tctaaacatt
    17461 ccattattgc cgcttttgga gttccataaa aattatcata aatttgagca tactccaaaa
    17521 actcaccttt ttctatcatc tcttcaaact tttccttact tatgaagaaa taatttactc
    17581 catcaacttc tccagttctg ggttttcttg tagtagctga tacagaaagt tttattgtat
    17641 cattttcttt aagtagttct ttgcaaatag taccttttcc tgcacctgat ggtcctgata
    17701 caactagcaa taatcctttt ttcacaaaca tttaagcatt ccccttctaa tattgtattc
    17761 ttactctata ttttgtattt gttctcttat tttttccaat tcgcttttta cttctactac
    17821 taaattagtt atatttatat cagatgactt cgaacctata gtgttagttt ctctattcat
    17881 ctcttgtatt aagaaatcta tttttctacc tatagagtcg tctttaaata tagtattttt
    17941 aagttgccct atatgacttt taaatctaac tatttcttct gttatactgc ttttatctgc
    18001 atatattgca acttcttgtg ctaatctatt ctcatctatt atacttgggt catccagtaa
    18061 ttcacttatt ctcaaattca atttttctct ataatcttct acaacactac ttgaatattt
    18121 ttcaatctct tctatatgat tttttaataa atcacatctc atggcaatat cttcagctaa
    18181 ttttttacct tcttcacttc tcatttctct taatttgatt aatgcatctt caactgcttg
    18241 attcaacatt gaccacaata aatcttcatc ttcttccttt tcttctattt tcacaatatc
    18301 aggaaacttt gcaacattca taacgctaat atcatctata atatcaaatt tattttttat
    18361 ttcttttagt atgtctatat actgagatgc taatccctca tcaaaattta agtttacatc
    18421 ttctttgcct aataagtcaa actttatgta taaatctact cttcctcttt ttacataatt
    18481 ttttactaaa tttctaactt tatcctctaa aaatgaaatt tttcttggaa gacgtatgtt
    18541 tatatctgaa tacttatgat ttatcgtttt acactctact aaaaagtaat aattatcatc
    18601 cttgtattct cctcttccaa aaccagtcat acttatagcc attaattaaa cctccaaaat
    18661 accttcacat atttttactg ctggacctgt catataagag aaatctccta catctatttt
    18721 aactgttcca ccttgagacg ttacatttac actactttct accatattaa gtaaattgca
    18781 cactattgct gatgcagtca taccagtacc acaacctaaa gtatatccac aacctctttc
    18841 ccaagtataa acatgtatat tatacttatc ttctaccttt acaaaattta cattcgtttt
    18901 ttcaggaaat attttattat tttcaatctc ttttccatat ttacaaacat catctacatc
    18961 taattcatct actataacta ctgtatgagg aacccccatc aacatagaag taactttaag
    19021 ctccctatct aaaaccttta tactttcttg cacaaaacgt tctttatttg taagtacagg
    19081 aacgtcctta gctataaaac ttccttttcc catattgact cttatagagt ttatttctct
    19141 attagtaaga tttactttta tatcttttat accatctaat gtatctactg taaaactttc
    19201 ttttttaact atacaattat cataaacaaa tttacaaaaa catctaaggc catttccaca
    19261 catagctgct cttgaaccat cagaattata atataccatt tctacatctg caacttttga
    19321 atctttcaca actaacaatc catccgctcc tacagaaaat cttctatggc atactttttt
    19381 tgctaaagca gtataatcag atggatttat ctggtcgaat cttccatcta ttgctataaa
    19441 atcatttcct attccatgta gtttccaaaa tttcataagt atcgctcctt cgtaaattaa
    19501 cacttaaact attataacat aaaaaaattc ccataaactt ataatttatg ggaaaagttt
    19561 tgtaatgaca actataattt gaggatgtga gggagagttg ccattacaat tagggtaatt
    19621 atattatgtt tttaagctag ttctttctat gtttatatat taatatttct atatgaattt
    19681 tatgtatgtt ttatttaaac tgtctatgaa ttttttaatt aaacatctgg taactagcta
    19741 taatccttct agtagatatc tattaacata cacctattaa ttctttaagt gtttttaaaa
    19801 catagtctaa atcttcctta gtaatatcta catgagttac aaatctatat tcaccatttt
    19861 ccattttatt aatttttata tttctttcaa aaaattcttt tattaaaaca tcctctttta
    19921 taatatcttc actcatctta aaaaatacca tacttatatc tggattaaca ttgtttactt
    19981 caatgccatc aattttattt aattcacaag ctaaatattt tgcattttca tggtcttcat
    20041 tcaatcttaa agtcattttt tcaatggcaa ttataccagc tgctgcaact atacctactt
    20101 gtctcattcc tccacccata actttttgct ttcttctagc ttttgctata aattctttat
    20161 ttccagcaag tatagaaccc attggtgcag ctaaaccttt tgacacacag aacataacac
    20221 tatcacaaca ctttgctatt tctttagctt ctacacccaa agttaatgct gcgttaaata
    20281 atcttgctcc atctaaatgt actggtaagt tatgtttctc agctattttt ttaatttctc
    20341 ttaaattatc aagactcaca actgttccac ctgaatatgc attttctaca catataagcc
    20401 ctgtctgtgg ttcatgtatg tcatctcttc taattgcctt ttcaacaagt tcaggattta
    20461 gagctccact ttcgtcttca tctatagttc taagctgaac tccagctatt acagcaggtg
    20521 ccccaacctc atgagtcaca atgtgataac ctttaccaat aattatttct tgccctctta
    20581 ggcaatgagt aaatagagct aattgattcc caaaagttcc acttggaagg aaaatagcat
    20641 cttcttttcc aactttagct gctgctaatt cttcaagttt atttacagta gggtcatctc
    20701 taaagattga atctccaaca ggagcctttg ccatagcttc tctcatttcg tcagtaggca
    20761 tagtaacagt atcacttctt aaatcgataa atctcatttt ttcaaccttc tttcatattt
    20821 atatactata gaaccttaaa attgattttt tctgtaaagt tcatatactt tcatagctta
    20881 tactctacac aataattata ctatatttac tttatattta atctacttta aattattaat
    20941 tttaaaaata aagtttcctc tctaataaat ataaataatt atacaattta aatattaatc
    21001 tctattgttt aaattccccc tagtcacaaa gcgtatatta tgtgtataat taatttactg
    21061 actcattttg gtcaaagaaa ggggtgctat aaatggcatt agatggatta gttatacatt
    21121 caatagtcga tgaattatca tcaaaactta caggtggaaa aatagataag atacatcaac
    21181 ctgaagatga tgaggtaatt tttaatataa gaaataataa agaaaatttt agattggttt
    21241 taagtgcaag tgcctcaaat cctagagttt atcttacaag taattatcaa aaagaaaacc
    21301 ctctaaaagc gcctatgttt tgtatgttac ttagaaaata catacaaggt ggaaatatag
    21361 tagaaatttc tcaaataggt tttgaaagaa taataaaaat aagtgttgaa tctttagatg
    21421 aattaaaaga aaagacagtt aaaaatatta tgattgaaat tatgggaaga catagcaaca
    21481 taataataac tcatggtgaa gaaaataaaa ttatagattc tataaaaaga gtccctttta
    21541 gcataagtag agttcgtcaa gttttacctg gtcatgatta ttcattgcca ccagaacaaa
    21601 acaaactcaa ccctcttgat gatatatcta aggatttgtt tattaaaaac ctagaggaat
    21661 tggaaggacc tatctttaag tctatatatt ctaaatttct aggtataagc cctattatag
    21721 ctaaagaaat ttgctacaga gcaggtgtaa atcaaaatgc tatcataaag gatatatctg
    21781 atgaacaatt tgattcttta cacaaagtat tttgtaattt attcaatgat ataaattcta
    21841 acaaatactc tccttgcatt attatagata aaaaggtgga taaggtagtt gatttcagtt
    21901 gtataaacct tacattattt agcgatttat catatataaa taaggatagt atgtctcgta
    21961 ttctggaaga tttctataga actaaggata ttaaagatag aattaatcaa cgttcatctg
    22021 atttaaagaa gagtatatct gttaagcttg atagacttta taataaactt aaaaaacaag
    22081 aagaagaatt atcagaatcc gaaaatgcag acatttataa gataaaagga gaattgatta
    22141 cttcatatat ttatatggta gaaaaaggta tggaaagtat tgaagttgct aacttctatg
    22201 atgaaaattg taatgatgta acaatagaat taaataaaaa cttgacacca tctgaaaatg
    22261 ctcaaaaata ctttaaaaag tataacaaaa tgaaacatgc taaagtcgag atatctcatc
    22321 aaatttcctt aaataaggaa gaaatagatt acttagaaaa cataatttta agtattgaaa
    22381 actgtgaaaa tttagctgaa ttacaagata taaaagaaga acttgctaag gttggataca
    22441 tcaagactca aaagaaaaac agtaaaaagg atactatccc atctacaaaa cctcatgaat
    22501 ttgtttcttc tgatggattt aaaatattgg ttggtaaaaa caataagcaa aatgactatc
    22561 ttacactaag acttgctgat aatgatgact tatggatgca tacaaagaat attcctggtt
    22621 ctcatgtaat aataaagtgt gctggtaagg aagtccctga taatactgta tttgaaggag
    22681 ctatgctagc agcattcttt agtaagtcaa aactttcttc tcaggtgcct gtagactata
    22741 ctaagagaaa aaatgtgaaa aaacctagtg gttctaaacc aggtatggtt atttatgaaa
    22801 ctaacagtac tatttatgtt actcctgaag aagaaacagt agccaagctt aaggttaaat
    22861 ctgaataaat tctgcactaa atacaaaata aatgttagac aactacacat tatcattcta
    22921 aataaaaata gtggccttct tataaagtac aataataagg ccactttcta attagcttac
    22981 ttttaatttt ctaacaaaat acttttctgt tttaataact atacttatat tcccaacaaa
    23041 tattttcagt cttcttatat agatgtaatt aaaaagttaa cactaaaagc tattttttaa
    23101 tttattgatt tgttttagta attcattata ttctgattca tacaattctt tttttagatt
    23161 atctttttct atagataaca tagatataac agttgtaagt ctattttcta atactagaag
    23221 attttctttt ttgatttggt tttctctact aggagttttt ttatttctgc taattgtata
    23281 ttcgtcataa ttacaatcaa actcccttat tttagcatct tttatctcta taatatagtt
    23341 acaaatatgt gatacaaata ctctatcatg agacactata agcatagttt tatcagtatt
    23401 tatcagtgcc ttttccagtg cttccataga ttttatatct aagtaatttg ttggttcatc
    23461 aaatactaaa aaattattat cttccaatat tattttacat agtgctattt ttaccttttc
    23521 tccaccacta agtactttta cttttttgta gacatcatca cctttaaacc caaataggct
    23581 taagtttatc cttataaaag attcatcaaa tgaactatta actttagtat tgtatagaac
    23641 actcttttcc tcatctaata aactttgatt ttggtcaaaa taacctactt ttaccttgtt
    23701 atttattttt atattgtcat ttttatcagc caatatttct tttatcagag tactttttcc
    23761 acacccatta tcacctaata atgctatctt tttacctctt tttatcttaa aggaaacatt
    23821 atctaacagg agcttatttt ctaactttag agtcatatct tttacctcta ctagattttt
    23881 actaattatc tccataccat cttgaatctt aatgttcatt tctttagaaa ctttaggttt
    23941 attctttacc tcaagtttat caattctgct ttttatagcc ttcacatttg agtccaactt
    24001 tttcttatta gtctgaccac ccattttgtg caacctagct tctgaattac ccattctttt
    24061 tggtgttttt cttatgccat cacttaaagc ttttttatat aaaagagctt tttcaagcct
    24121 ttttttctct ttaacatatt cttgatactc aaaatcagct ctttttactt cttcatcttt
    24181 ttgcattaaa taatcagtgt aattgccttt gtaagctttt agttttccat cttctagttc
    24241 taatatagtt gtacataact catccagaaa acgtctatca tgagatatta ataataatgc
    24301 tccctcatgc cttttaaaca tatcttccaa aaccccaatg ctttttttat ctaagtttga
    24361 agttggctca tcagctatta tgatattttt cttattactc agcgcatctg ctatttttaa
    24421 cttaaccttt tcccctcctg ataagtgttc ttcaaattta tctggagcat ttaaaagact
    24481 cttctcctta ctatgactac atgtttcaat attattttca ctctgactta tataagaata
    24541 gctttctgta aggtaagtat atccttcatc aatttctatt tctcccaata ttgcttttaa
    24601 taatgttgtt tttccttgac catttgaacc aactagacct attttgtcat tttctaatat
    24661 ttcaagttta tctatatcca aaattaattt atctgcataa tatttcttta aattctctac
    24721 ttttactaac aacataaaaa atccctcctt aacacactta aatataagga gagattctat
    24781 aaataggcat aacaaataaa cgtatagact ataatctact ctccttaatt taagcattgc
    24841 aaataaagcc accaaaatag gcttttcaaa aattacaata ctttaaatta taaaaaatag
    24901 attatattct cattgtccta gttaccttgc cctttctttc atttattttt aatcattata
    24961 tcaacattaa ttacaaatgt ctatagaaaa ttaactaatt ttagttattc aatatacaac
    25021 ttattcttta aatttattct tcttcaactt taaagtcctg attatttaaa attctgttta
    25081 atacaattcc aacaattgca gctaaactaa gaccagttat ctttactgta cttgttatag
    25141 ggattccaac agaaatacct ttattagata aatatgttgt tccaagacct attaataata
    25201 taactgctat gattaatata tttattttac tttctacaca agaactgtcc cttatagttt
    25261 ttaatcctac ataagttatc atcgaaaata gcattatact aattccaccc ataactgaac
    25321 ctggtatact ttgaagaaat ccaccaaact tgcctacaca tgcaagcaat atagcaaata
    25381 ttgctgtacg tctaagtatt gaagggtcat agtttttagt tatagcaagt actgctgtat
    25441 tttctccata agttgtattt gctggtcctc ctaaacaccc tgcaactata gtcgcaaatc
    25501 catccccaag taatgttcta tttaatccag gattttctat aaagttttta cctactacag
    25561 caccattagt agtcatatct cctatatgtt ccataaatac agctaataca actggagata
    25621 taatcactat tgctcctaaa ctgaacttag gaactgtaaa gttaggtatt gcaaataaac
    25681 ttgcttctgt aatagtagtt atatctacta atcttaatat caaacagatt atatacccag
    25741 aaaaaactgc tattaatatt cctaattgtt ttataaatcc tctaccaaac ttattggtta
    25801 aaattgctat tgctaaagtt ataaatgcta tcacaaaatt ggctgaagcc atactaaaag
    25861 ctgttggcaa taaatttaat cctataacta ctatcatagc tcctgtaact tgtgcaggaa
    25921 aatataattt aattttattt acaccaacta cttttactat aatcgacatt ataatataaa
    25981 ttaatcctgc tactattata ccaccttgag catatgctaa atctccacca tacgcctctt
    26041 tagctgctag tataactggt ataaacgcaa aacttgaacc taaaaaggca ggtacttttc
    26101 cttctgtaca aaaatgaaat atcaatgtac caacaccagc acagaaaatt gcaactgacg
    26161 gattaaaacc tgtcaaaatg ggaactaata ctgtcgctcc aaacatggct aatagatgtt
    26221 gtaaagacat tatcgttttt ttcataaaaa cctccaaaat tattatatat aaataaatcc
    26281 tctgtttaat taataccttt taattaatta tcattcccta gattctattt tttaacctct
    26341 atatacttat tatataattt atcattattt tacattttac gacatcaaaa actcaaataa
    26401 aggagaataa aaactacatt aagactcaca tccataaaat tataatattt attttgtgca
    26461 atctatgtaa gctttatcct cctccctgac tattcaatgc taagctttta tataatactt
    26521 aaaaataatt attctttgat tgtaactgaa tcttctccat caatttctaa taaactgaca
    26581 gatataattt catgtctaga agtaggtaca ttttttccta cataatctgc tcttataggc
    26641 aactctctat gccctctgtc cactagcact gctaattgta ttgatttagg tcttcctata
    26701 tctataatag catctagtga agctctcaca gttcttccag tgtataaaac atcatcaact
    26761 aaaactactg tcttatcatt tacattaaaa tctaaataag ttcctttaac tacaggttca
    26821 acatgaattt tttccaaatc atctctatac aaagttatat caatatctcc tgtatctact
    26881 tttgttcctt ctatttgttc tattttctta gaaattctat ttgcaattgg cacacctcta
    26941 gtctttattc caactaaaac tagattttct acacctttat ttctttcaat tatttcatga
    27001 cttattctag taattgctct tgctattgcc ttttcatcca ttaattgagc tttttctacc
    27061 atatcataat tattgtcaaa gatataatat atctatttga caaatctact cctttctttt
    27121 aattttttat aaatttattt ttatttaaaa tttcaattta aacaacaaaa aaacttctca
    27181 tcacaagaca agaagctctt atataaaaaa tatacagtgt aatttagcaa ttgtcttacg
    27241 atatctctgt atcgtttaaa gataattttt taatctatca actcaaatta tatatttata
    27301 tttcaaattt ttcaagcttt tttaaacaaa atactttaat ataagatatt ttatttaaat
    27361 ttcactaaat ttatttactt atataaaaac aaaaaatgat ttataagtat tatacaaatg
    27421 gttattttat ctttttaata acttccttaa aatactcagg taattctgaa ttaaattcga
    27481 tatattcctt agtagtagga tgtataaatc ccaacttctt agcgtgcaaa gtttgtcctt
    27541 ttaatttaaa tttagtttct tttggaccat atacataatc acctaaaagt gggtgattta
    27601 tagacaaagc atgaactctt atttgatgag ttctaccagt ttcaagccta gctctcatat
    27661 gtgtaaactt atcaaatctt tctacaactt caaagtgagt gattgcattt tttccatctt
    27721 ttactacagc catttttaat ctatccttag gatttctccc tataggttta tcaacagtta
    27781 ttttatcttc tttaaccaca ccatagcata taaattcata ctccctagtt atagtatgtt
    27841 cctttaactg ttctgctaga aaattatgtg cataattgtt cttagctatc ataagaagac
    27901 cagaagtatc tttgtctatt ctatgaacta taccaggtct aataactcca tttattgatg
    27961 acaacttatc tttacaatga tataaaatcc catttactaa cgtattttca tagtttccag
    28021 gagcaggatg gacaaccata tcctgaggtt tatttattat taagacatca tcatcttcat
    28081 atacaatatc tattggaata tcttgaggta aaacttctaa tactttaggt tctggtatct
    28141 ctataacaat attatcttcc tctttaacca gatatctagc tttttctact ttattattaa
    28201 cctttacttt tccatctttt ataatttttt gtatataact tcttgacata tcacctaatt
    28261 gttctgctag ataaatatca agtctaactt cttcttcctc ttcaagtact aaaaactctt
    28321 ttacttcatc cattatatca cctactttta ctttcaaaaa ataaaacata tatacataaa
    28381 aacacagttc ctacaactac aaatacatca gctacattga atacatattc ccatataact
    28441 ctaaagtcga agtaatctac cacaaaacct aatcgtactc tatcaactag atttcccaat
    28501 gcaccagata taattaatat aattcccaac cttccaaata tatgtacttt ccttgtatta
    28561 agatagtata atccaaatac cgttgcaagt aatgcaacaa ttataaatat ccattgatta
    28621 ttttgtaata atccaaatgc tgcacctcta ttttcgacat aagttaaatg aaatacatta
    28681 tttattattg gtattgttga tacatccacc aaattattca atacccatat tttagacagt
    28741 tggtctaaac ctatgagtag aattattatt aatatatata gcaatgattt ttccccctat
    28801 aaattattta aaaaccaatt ttatttgaag cacactatta ttattataca aaaaaaatgc
    28861 tctaaagagc aactttatta aaattgtatt ttcttaataa agctagaaca aaatttaaaa
    28921 tagtactact ttttttatat tgtagatttc tacatattta aaatcatatc attatgtata
    28981 aaaaatttaa aaataccata ctaatcaata ttttttgtgt attttataga ttaaagatta
    29041 atgatgtctg taatttaaac ttacaatcac cactaatctt caaacaaata tttttacttt
    29101 tgcacttgct tatttttatc taaatgttgt ataagttcag atactgttac gaatttatat
    29161 cctctctctt ttaatttagg aattactact tctaaagctt gtatagtttg tgatttatca
    29221 tttcttttag tgttataatc atgtaaaagt attatattac cattttggac ctttgtaagg
    29281 atagtattta taattgcatc tactccaggg ttagaccaat ctctagggtc taagtttgac
    29341 caaagaatta tattcattcc tttagaaact ataatatcac agacactttt actcatggct
    29401 ctatatggag gtctaaatat ctttggttct actcctatca cactttttat aatattttgc
    29461 gtatctgtta tttctttttc aacactacta taactatttt ttgcaacatt tatatgtgta
    29521 aatgtatgat tccctatttc atgcccctca tcatattgtc tttttaataa ttctttgtta
    29581 tattctactt tttctccaac tacaaaaaaa gttgctttta catcattttt ctttaatata
    29641 tcaagtactt gtggtgtaaa atcctcatct ggaccatcat caaaagtcaa tgctaaaatt
    29701 ttttcatcct catttccttt ttttattatt acatcttcat aatcgtcttt tatattgtca
    29761 tttaaactat atgcttcttt tgaactatct tgattatgtg tattatgatt atatgtatat
    29821 acacctacta tagataataa acatatacat ataccacata ttattttctt catgtcatta
    29881 cctcttttct aattcattct agcaatatat tttataatgt cagtttttta atctctcaat
    29941 attccatttt tatttaaatc agttaaattt gttaggtctt ctgaataaaa gttagaatct
    30001 tctacatcag ggttttgcat tgcatcacct aataaattat tttccttctt tgcacaatca
    30061 ttacataaac ttgtatctgg caatatatcc aatctttcta aatctatttg attatgacat
    30121 aaatcacaaa tcccatatgt tccattctct attttagata atgctaagtc tatttgatac
    30181 aatcttccct cttcatgatt tattagactg ttttgcatat tttgcatata taaatctgtt
    30241 cccatgtctc ctatatgatt atcataacta gataattctc ctgatgaata cttttcactt
    30301 gtatgtttag ttgtatctcc aaataaagta ttatctttca tatcctctac taaattagtt
    30361 aaattttctc tttctttcaa taatttatct ttatattttt tagtatccat ttttaacacc
    30421 tctaatttta atatttacat cataaaaata gtatgtggaa aagtaaatta aacatacaat
    30481 aaaaataaga tttgattcga aaaatggaat gtctcaaaat gaaattttta gttcatttaa
    30541 ccctcttttt tatatgaaat caaatatatt tttatccttt cagcaataaa aaagagactt
    30601 atctctattt tgagatagtc tcttctttga acataattta aatacaattt tttaattttt
    30661 atagttatta ttttactaac ttgccaattt atgattattt tgttctttaa tatgttttgc
    30721 ttttttaaag aataaaagac caagaattat tgctacaata acacctattc cattagatat
    30781 attaactggt agtctaaatc cttcttttgc ttgcattata taactaaatg ttactacagt
    30841 cataaaaagt gctggtaatg tagttatata atggttttta tttgctttga ctaaatatgc
    30901 agaaccagtc cacaacatta tcattgcaag tgtttgattt gcccaagaga aatatctcca
    30961 aattatatta aaatctataa atgtaagagc aacaccaact gcgaataatg gtaaagctat
    31021 taaaaatcta tttttgactg catcttgatt ataattcatt gcatctgcta ttgtcagcct
    31081 agcacttcta aatgctgtat ctccagaagt tataggacat gccacaactc caagtatagc
    31141 taaagctcct ccaataggcc ccattaatcc aacagatact gtatttacta ctgttgctgc
    31201 tccaccatca ttaaatatca cattaagttg aggtatgcca tggaaatatg acatggctgc
    31261 tgctgcccaa actaaagcta ttataccttc tgcaaccatt gacccataaa atactggtct
    31321 tgcctcactc tctttctcaa cacatctcgc cataagtggt gattgagttg catgaaaacc
    31381 tgatattgct ccacatgcta ttgttataaa taagtatggg aataaaggtg ttccatttgg
    31441 attcatattt tgtaaattta tatttggtat gttatacccc tttattatta atcctccagc
    31501 tacacctact gccataatta ataatgctgc tccaaataaa ggatatattt taccaatcaa
    31561 tttatctatt ggtaaaacag tagccataat ataataaagt attatcactc caacccatat
    31621 ttgtgcgttt acacctgtca atgtctttaa taatcctgct ggacctgtta caaatacaac
    31681 tccaaccaat actaaaagaa taacactaaa tattctcatt gctatttttg caccatttcc
    31741 tagataaagg cctaccaact ctgatgcaga tgtacctttg tttttcattg atagatatcc
    31801 tgaagcaaaa tcgtgtactc caccagcaaa gattgttcca aacacaatcc ataaaaatgc
    31861 tgatggtcca aataatgctc cttgtatagc tccaaatatc ggacctaacc ctgctatatt
    31921 tagaaactga attaaaaaga ttctcttcca tttcattggc acataatcta ctccatccct
    31981 acaagctata gctggagttt gatttttgtc attggttcca aaaacctttt ctacaaaagt
    32041 accatagaca aaataaccta ctataagtac tactattgaa cctaaaaagc ttaccataaa
    32101 ttcatcccct tagtttttat ataattgctt tataaatttt tatgagttta agaaaactat
    32161 ttccagttca aatagtaata cttttttatg ctaatataaa tattaattac attaagtgca
    32221 tatttaagta catgaaatga ctttatatat gcattaacag cctaaattct tttactttgc
    32281 ttctacttac agttacttca taatcactat tttttaattt taatatgtat gtattattaa
    32341 accatggtat aatttctttt actttggtta agtttacgat ataagaccta tgtgttctaa
    32401 aaaatgtttt atcatttata agattttcga cttcttatat gccttctttt aatataaact
    32461 tttctttctc agtatatata aatgtctgcc tttcattagc ttcacagtaa tatatatcat
    32521 caatatctat tacaactaat ttatcacctt tccaaaggct aattttatgt gttatttctt
    32581 ctatctcctg atttactgct tccttcttat atttgtataa attactattt acattaaata
    32641 gttcaatatc actcatctca cttttttcta atttattcag catagaaata attctctctt
    32701 cagaatatgg tttcaatata taatcaaata catccaaaga aaaagcatct actgcataac
    32761 tttcatatgc tgttataaaa acaattttag gtcttgattt aaactgattt aatgtttttg
    32821 caagtagcat tccatcaaga tgaggtatat ttatatctaa aaaaattaca tctattttat
    32881 tttcctgaat aaagtctaac acctctatac cattggtaaa ttcacttact acctcgattc
    32941 cacttttatt ttcaataaaa tatctcaatt cctttcttgc tggaaattca tcttccacta
    33001 ttatagctct catacttcta ctcctttaca taaaatacta tcaaagtccc attatctatt
    33061 cttcttatat taagccctct tccatacatc agtttaagcc ttaaatgtac attataaagc
    33121 ccaatcttat tttctggcat attatcttga tatacattat ttataatttc ctcacttata
    33181 cctataccat tatcctctat tgatattctc acagtatttt ctaatgaata cggtaacttt
    33241 tttatggata ttttcactac accagctcta ccactttcta aaataccatg tattattgca
    33301 ttttccacta atggttgtat aatcaaactt ggaatcttta tatttacatc ttctatatca
    33361 taagatacag ttagtaattc tccaaaccta gctttttcta tctctacaaa agatttaact
    33421 tgttcaatct ctttatttat atcaattaag ttatcactat attcaagatt atatcttaga
    33481 taagttgata aatttattat taactctcta gccttatctg gactaaatct tataaatgaa
    33541 gctattgtgt ttagggaatt aaacaaaaaa tgtggattaa tttgtctttg aagtgctttt
    33601 atttctgctt tatttctcgc ttctatatct tttttatctt tctcaatccc ctctactata
    33661 gatataacaa atcctattcc aatctgccca actattaaag gtaaatatat tccaccgatt
    33721 atttgaattg ctaaagaatg tggatatgat attaagtata taagtaatat agtaaaactt
    33781 tcagaaatca tgcctaccaa tattccatac attactctat gttgttttgg tatcctctta
    33841 tatagaaatc ctgataatac acctgctagt atacttgata aaagacatgg aacagatgtt
    33901 atccccccaa tatccataaa atatctatga atccctgaaa aaactcctgc tgtaatacct
    33961 accatcggac caaataaaat gccacctgat actatagata ttattctagt gtttactatt
    34021 gaccccatat aatttatacc actataagtt ccaaatatgg ctaaaaaagt aaaaactaat
    34081 gctatacaga ccaagtcatt aaaactatat tcttcctttt gaaatatctg cttaaatatt
    34141 tttaactttg ttattagaag aaacaatact acaataaggc ttgtcttttc tattaatgcg
    34201 attaaaattg atgttttcat aatccccctt taaaaaataa atttttacac actctaaatt
    34261 tattatggtt gttataaatt acatatttga agtaatccta tcacaaaact ttccacattt
    34321 aatcttttaa ttaaataaga aatagttgta cttctattaa caactaatac tagctagcat
    34381 aagcttgtaa taacttgtag ttattattaa ctttacaatt ctagtattta attttatact
    34441 ttagaaatct cttttcttag aaataatact ttcgaatatc atataataca atattatgta
    34501 gattcttact catacaacat atttctttaa aaattttata ctattctttg ttcaaatatc
    34561 tcatttgatt taattgtgtt aaacacacat tatcatctct ctgttatata tttattatat
    34621 agccgtatat cgataaagta aataaatccc atatttaaaa taatatgttt gacaataaat
    34681 tattataatg tatatttacc ataataattt gtcaaccttg aaagttactt ctataattat
    34741 tgttaaataa tt
//
LOCUS       DAYASV010000028        33977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYASV010000028 DAYASV010000000
VERSION     DAYASV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.028"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            91..822
                     /locus_tag="ACGI68_001385"
     CDS             91..822
                     /locus_tag="ACGI68_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0137829.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            824..2182
                     /locus_tag="ACGI68_001386"
     CDS             824..2182
                     /locus_tag="ACGI68_001386"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0137830.1"
                     /translation="MINLFSSDLFLKYSFFFSFIVYRILMVIVQYYFLINFTNFNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            2272..2706
                     /locus_tag="ACGI68_001387"
     CDS             2272..2706
                     /locus_tag="ACGI68_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137831.1"
                     /translation="MKVGNINGQAPITNGLDSCQSEVQGLEQKKKALQDEIEKVRSDK
                     SIDGETAKLKIEALKKEMEEIENKIQELKGNKGKKTNKEENNVSEVNNNLLERKKEPS
                     NNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRPI"
     gene            complement(3361..3585)
                     /locus_tag="ACGI68_001388"
     CDS             complement(3361..3585)
                     /locus_tag="ACGI68_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0137832.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(3682..4935)
                     /locus_tag="ACGI68_001389"
     CDS             complement(3682..4935)
                     /locus_tag="ACGI68_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFH0137833.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            5195..6184
                     /gene="add"
                     /locus_tag="ACGI68_001390"
     CDS             5195..6184
                     /gene="add"
                     /locus_tag="ACGI68_001390"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFH0137834.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            6256..6996
                     /locus_tag="ACGI68_001391"
     CDS             6256..6996
                     /locus_tag="ACGI68_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HFH0137835.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPSLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            7196..8347
                     /locus_tag="ACGI68_001392"
     CDS             7196..8347
                     /locus_tag="ACGI68_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFH0137836.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            8372..9127
                     /locus_tag="ACGI68_001393"
     CDS             8372..9127
                     /locus_tag="ACGI68_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HFH0137837.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            9319..11706
                     /locus_tag="ACGI68_001394"
     CDS             9319..11706
                     /locus_tag="ACGI68_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HFH0137838.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGRTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(11937..12170)
                     /locus_tag="ACGI68_001395"
     CDS             complement(11937..12170)
                     /locus_tag="ACGI68_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFH0137839.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            12459..13049
                     /locus_tag="ACGI68_001396"
     CDS             12459..13049
                     /locus_tag="ACGI68_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0137840.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEHFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            13163..14008
                     /locus_tag="ACGI68_001397"
     CDS             13163..14008
                     /locus_tag="ACGI68_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137841.1"
                     /translation="MIKVDDLSFSYTERDFLQNINFGVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENEANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            14005..14688
                     /locus_tag="ACGI68_001398"
     CDS             14005..14688
                     /locus_tag="ACGI68_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137842.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            14685..15401
                     /locus_tag="ACGI68_001399"
     CDS             14685..15401
                     /locus_tag="ACGI68_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0137843.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISFLLNLSISLLVLLQGIIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            15498..15761
                     /locus_tag="ACGI68_001400"
     CDS             15498..15761
                     /locus_tag="ACGI68_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0137844.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            15733..16188
                     /gene="def"
                     /locus_tag="ACGI68_001401"
     CDS             15733..16188
                     /gene="def"
                     /locus_tag="ACGI68_001401"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFH0137845.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            16240..16701
                     /locus_tag="ACGI68_001402"
     CDS             16240..16701
                     /locus_tag="ACGI68_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HFH0137846.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            16751..17653
                     /locus_tag="ACGI68_001403"
     CDS             16751..17653
                     /locus_tag="ACGI68_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0137847.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSEGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            18005..18835
                     /locus_tag="ACGI68_001404"
     CDS             18005..18835
                     /locus_tag="ACGI68_001404"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0137848.1"
                     /translation="MLNKLCMYLERPELYKQSEINFWDDEYISEQLLKAHLDTNFEGA
                     SRNFNFIEDSVNWIVTVANPVNYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSIN
                     YAQNRNKETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSL
                     KPGGKLILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTL
                     VITEKDENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK
                     "
     gene            19245..19379
                     /locus_tag="ACGI68_001405"
     CDS             19245..19379
                     /locus_tag="ACGI68_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137849.1"
                     /translation="MDLINTIDKVQQAHKPIDEIIETIRDIVDIIKILEPVYNFKSNQ
                     "
     gene            19884..20645
                     /locus_tag="ACGI68_001406"
     CDS             19884..20645
                     /locus_tag="ACGI68_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0137850.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLARILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            20710..21492
                     /locus_tag="ACGI68_001407"
     CDS             20710..21492
                     /locus_tag="ACGI68_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFH0137851.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKCKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            21759..22178
                     /locus_tag="ACGI68_001408"
     CDS             21759..22178
                     /locus_tag="ACGI68_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HFH0137852.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(22281..22547)
                     /locus_tag="ACGI68_001409"
     CDS             complement(22281..22547)
                     /locus_tag="ACGI68_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFH0137853.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            22774..23475
                     /locus_tag="ACGI68_001410"
     CDS             22774..23475
                     /locus_tag="ACGI68_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0137854.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            23557..24165
                     /locus_tag="ACGI68_001411"
     CDS             23557..24165
                     /locus_tag="ACGI68_001411"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0137855.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            24489..24953
                     /locus_tag="ACGI68_001412"
     CDS             24489..24953
                     /locus_tag="ACGI68_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HFH0137856.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTYETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            25590..26606
                     /gene="gap"
                     /locus_tag="ACGI68_001413"
     CDS             25590..26606
                     /gene="gap"
                     /locus_tag="ACGI68_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0137857.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            27001..28170
                     /locus_tag="ACGI68_001414"
     CDS             27001..28170
                     /locus_tag="ACGI68_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137858.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYSPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            28550..32803
                     /locus_tag="ACGI68_001415"
     CDS             28550..32803
                     /locus_tag="ACGI68_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HFH0137859.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            32952..33446
                     /locus_tag="ACGI68_001416"
     CDS             32952..33446
                     /locus_tag="ACGI68_001416"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFH0137860.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            33562..33939
                     /locus_tag="ACGI68_001417"
     CDS             33562..33939
                     /locus_tag="ACGI68_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFH0137861.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
BASE COUNT        13662 a         3437 c         5700 g        11178 t
ORIGIN      
        1 gcgagattat tacattttgg aacaaatatt caacatatta gatcatatta attgaacaga
       61 aaaaattaac tttatataat aattagaata atgataagaa agaaggtgta tatattgatt
      121 tcaattgcca tttgtgatga tgaatcctat atgctaaatc atttaaagga aaatatagaa
      181 ttatacatga agcagaagaa tttattatat aatatagagc tttttaataa agtagaagat
      241 ttactatgca atcatgaaaa gtttaatatt gtatttttag atatacagat ggaagagatt
      301 aacggaatgc aggcagctag aaaattaagg tcatatggta atgagtgctt tataattttc
      361 gttacagttt taaatgaatt tgtatttgat gcttttgaag tagatgctat aaattatctg
      421 ataaaacctc tatctaatga gaaactatat aaaactttgg ataaaatagt tacaaaactt
      481 gatgataatg aaagtaatta tatcacaata caaaaaggtt attcatattg gaaattaaaa
      541 cttgctgata ttttatattg tgaagttata aaaagaaaga tttatgttca tttaaagaaa
      601 gagactgtag attattatga gactattgaa aatttagaga gaagtttacc aacaaatttt
      661 tttaggtgtc atcgtagtta tataatcaat ttacaatatg tatatggata tgaaaatggt
      721 aatgcagtta tggaaagtgg agatagaata cctgtttcta gactacgtca acaaatattt
      781 tcaaaagtta tgcttgagtt catgaaaaat aggaggatat aagatgatta atttattttc
      841 ttccgattta tttttaaagt acagtttttt cttctctttt attgtatata gaattttaat
      901 ggtaatagtg caatactatt ttctaatcaa ttttacaaat tttaattcaa aattttggtg
      961 ttttatttta tattcgataa tttcaatttt tttaatcatt ataaatacat attttaattt
     1021 atataattta ttttcttcac agattctatt ttttcttatg acttttttat ttgtaaagta
     1081 cataataaaa caatctactg ttttatctat agtatcaact ttgttagtat atttaattga
     1141 acaattagta tatggattta cagctccatt aaattacttg atatttacta gtgatataaa
     1201 tacagatatt tataaatttg atataaatat gggactatct tctttaatta ctattataat
     1261 agctggattt atatattggt atagttctaa aaagtttaat attaaggtca tgaactttga
     1321 taaatatata gcaattttga tgattccatt gttgcttata atattattta tgcaatcatt
     1381 tgaatatagt tcaaatataa atatagatac aagtcttggg attgtaaagc tgctattaaa
     1441 tacttctata actgaagaaa ttcaagcttt tcttttttct ataattggaa ctatatgtgt
     1501 atttttttct ctatttacct ttaagaaact aattcaagct ctcgaagatg acaaggagag
     1561 ggcaattatg aatcaacaga ttcatgctca aaaaaactat attgaagaag cgaaatcacg
     1621 attatcacaa actatatcct ttagacatga ttttaataat catttagcta ttgtcaatgg
     1681 attgttgaaa aaagaccaaa ttttaaaagc acaagattat ttaaataagc ttgaaaaagt
     1741 aacaagtgac ctatcatttt cttgtaatac acagaatatt gttattgatg ctcttttcaa
     1801 caataaatta agtatagcaa aacaattagg catagaggta gattgtgaag taattatacc
     1861 ccaaagcact aacatgaacg attttgacct atgtatagtg tttgctaatg ctattgacaa
     1921 tgcaattaaa gcttgtagag ttgtagataa acaaaatagg tatttaaagc tttccactaa
     1981 gttagagggt ggatttttta tgatagaaat aaaaaatagc tataatccaa taaataatta
     2041 tacaagtggc tcaggtatag gtcttttaaa tataaaagaa attgctaaaa aatatcaagg
     2101 ggctataagt atagataaga catcagacta ttttcaaata aatgtattgc tagttatttc
     2161 attacattgc gctagtattt gagaacaatt tcattgaaaa tagtatttgt aagctatatt
     2221 atgtatttga aaagagaaag tttgatatat agaaatgagg aggttactaa aatgaaagtt
     2281 ggaaatataa atggtcaagc accaattaca aatggtttag atagttgtca aagtgaggta
     2341 caaggcttag aacaaaagaa aaaagcatta caggatgaaa tagagaaagt aagaagtgat
     2401 aaaagtattg atggtgaaac ggccaaatta aaaatagaag ctttaaaaaa agaaatggaa
     2461 gagattgaaa ataaaattca agagttaaaa ggaaataaag gaaaaaaaac aaataaagaa
     2521 gaaaataacg tttcagaagt aaataataat ctattagaga gaaaaaaaga accttctaat
     2581 aatgaaaatg taaaacaatt atatgatgtt tttgaaagaa gcaatgtaga cgaacaatgg
     2641 gataatactt atcgtgttct ccaagatgat aaaaacttaa aagtaaaatt taatagacct
     2701 atatagttta tttataaaaa tagttaatat tttaattatc accacttttt agaagaatac
     2761 acactttatt ttaaagaccc acatatttat ttacagttag cacatttata tgtggtttta
     2821 taactattat ttacaagtct atattgataa cttttacata gtatatgatg tttaaatact
     2881 tattcaaatt tatctataca taaacaaaaa aagagatttt aaaatctctt tttagattgt
     2941 tgacttatgg ctttttgatt taataaaatc aagaagccgt ttgaatttta tatgttggct
     3001 gatacccttc tgatattgaa acaccatttt tgtttccaaa atcgtttaaa attaagaact
     3061 tattatgaat gactatttca ttatgaacaa taagcaaata acatgaatct tcaatcaatg
     3121 ttttaacagt agcattaaat gcttgcttat atagttctat cgtttgtttg aactgcccat
     3181 taaagtaaat atgagtcaat aacataacta tatctacaca aattaatata aattttttat
     3241 actgtacatt atctaactgt tgattatctg tattacaaca tgattgaatt taaaaagcca
     3301 tcaacacata gaatgttgat ggcctgaaaa tagattttaa aatctctttt ttattatata
     3361 ttaattattt tttaaattta tattttttgc atcagatttt ctaatagcta agtcaaaccc
     3421 tctaaatcta atcactatag ggtcattaag aggagctatc tttttcactt caacttcagt
     3481 gccatctatt agtcctaagg ctagaagtct cttcataagt ttttcatttc cagctatatt
     3541 gtctataatt cccttttgtc caagttttaa gttgtaaaca gtcattatta acacctccta
     3601 atgaaaatca ttatcaaact ttatatttat aatatataac aaaaagagca aattgtcaat
     3661 atataacaaa aaataaacat actaagttag tatgtttata aaaaatagta tacatgggat
     3721 actaatagca tctagcaaaa aaccacttac taatggtaca actaaaaagg cttttggaga
     3781 atcaccatat ttttgtgtca aagaactcat atttgccaga gcgttcggag tagctcctat
     3841 tccatgtcca atcaaaccac ttattatgat ggcagaatca aaatcttttc caagtactct
     3901 aaagcaaata aatattgaaa ataggattat aaagattact tgaaaagtta ctattaagag
     3961 aataggaggt aataatgtag ataattcaaa taaatctata ttcataagtg ataaagttaa
     4021 aaaaattccc aatgaaagtt cttttaaaaa atctataagt ttaaaattta attctagtat
     4081 atttatctta tcatttaaat ttctaaaaat aacagccgaa aacatacaag ttacaacaga
     4141 gggtaatagt atattaaacg ttatatagaa gcatctactt ataatttctc ctgtatttat
     4201 acaaataaat atgagaagaa cctgttccaa aaatacagat ggtgttattg tttcggagaa
     4261 gttattattt tttatcctat tcttatttct aaaaaattta cttccaaatc tctttagatt
     4321 tctattatat ctaggaatcg acaagtctat atttgatgat ggttttaatt tatatttatt
     4381 tattagaaat ttagctacag gacatcccaa tataccaccc ataataagtc ctattgttgc
     4441 agcagataat ccaaccccaa ctgctccttc tataccaaga ttttctattg taagtccaaa
     4501 tgcagcagaa taaccatgac caccttccat agatacattt ccacacataa gccctaaaag
     4561 aggttcaagt tttataatct ttgatattgt aatagctaat atattttgac aaaatgccaa
     4621 tactccacac aaaatccaat atctaaataa tacactacca cctttcttta taagatttat
     4681 gcttatttca agtcctatag ttgtaaaaaa gaaggttatg aaatttggca ttaaatttcc
     4741 acttatagat atatctgcta tattaaaata tttaaaaaat aaatttagaa aacaaaataa
     4801 tagacctcct attactggtg caggtataca gaaattattt aaaaagttaa ctttattctt
     4861 caataaatta ccaattaaaa agaagaatat agatagtgtc aaggtttgca ctatatcaaa
     4921 acttaaggta atcaaaataa gtcctccttt gtaattcaaa gttttaatta tataacttaa
     4981 attcttatgt attgaaattt tattatcaaa tgtatattaa taaatgtata ttaaaaagaa
     5041 tcagtagata taattagata gtcataaatt aaattaaaat attttaatga gatatgactt
     5101 ttttagaatt aaaaaccgta gtatattgat ataatagaaa taagacttaa aaatctgttg
     5161 atagtattat ttataagtac agtaggagga taaaatgttt gaaaatttac ctaagataga
     5221 tttacattgc cacttagatg gaagtgttag agtagaaacc atgcttgata tagctattaa
     5281 agagaaaata gatttacctt caaataatat ggatgaaata aaaaaattag caaaggtttc
     5341 ttttaattgt acttcactag atgaatattt agaaaaattt gatttaccct taaaagtaat
     5401 gcaatctaag gaaaatttaa aaagaattac ttttgaactt ttggaagatg caagtagaga
     5461 aaatgtaaaa tatattgaaa ttagatttgc accattactt cacacccaaa aaggtatgag
     5521 tgttaaaaat ataattgagg gtataattga aggaattaga gaagctgaaa gtatttatga
     5581 tataaaaggc aatttaatat taggttgtat gagaacaatg acctcaaaag aggcattatt
     5641 agtaattgaa gaagggaaat catttgtaaa taaaggtgtt gtagcagtag acttgtgtgg
     5701 tccagagaaa gagggatttt gtaaggaata taaagatgtt tttaaattag caagagaata
     5761 tggatataaa gttacaatac atgcaggaga agctgcaagt ggagaaaatg tattggatgc
     5821 tataaatatt ttaaaggcgg ataggattgg tcatggggta aaaattaaag accataaaaa
     5881 ggcgtataat ttagttaaag ataaaaaaat attgcttgaa ttatgtccaa ctagtaatgt
     5941 acaaactaag acagtagatt cttatgaagc acatccattt tatacttttt ataaagataa
     6001 tttacatgta agtataaata ctgataatag aactgtatca gatataaatt taagttcaga
     6061 actaaatgtt atatttgata cttttaaact aggattagaa gactataaaa taatttatag
     6121 aaatgcagta gaagctagtt ttgcagataa agaaactaaa gagtatttga atagtttaat
     6181 ttaatagata aaaatattta aactttaaat taagctatta aattgataaa aatacattgt
     6241 attaggagaa aaaatgtgaa agattatgaa gatgttgttt atattaaact agtcaaaaaa
     6301 gaacttgaag aatggaagaa aaatatatta aagaaacctt ctatatttga caaagcatca
     6361 aagggtatac agaataaatt taatggagta ttgccagata aatatcatga aataattact
     6421 tcttctataa aagtaatgac aaaaaccgta ctgtttggat ataaatatgc tacaaagcca
     6481 tcacttagaa atatgtcatt agaagaaaga gaaaaattag ttttagaaaa agctaaaact
     6541 tacaagacta cagcaatgat tgaaggggca ggaacagggg caggaggaat cctcttaggt
     6601 ttatctgatt ttcctttgct tttaggtatt aaagtgaaat ttttatatga tgtagcttca
     6661 atatatggat atgatgttaa agattataga gaaaggattt atatattaaa tatattacag
     6721 ttggcttttt ctagtcaaca gcatataaac aaaactttta aattgatgaa taactgggat
     6781 gaatatgtaa atagtctacc agaagacatc aatgattttg attggagaac atttcaacaa
     6841 gagtataggg attatttaga tttagctaag tttttacaac taatgcctgg aataggagca
     6901 tttgtaggat tttatgtgaa tggaaaatta attgataaat tatgtgaaac tgctgtgtct
     6961 gcttatagat tgaggattcg agagcttaag ttataagaga aattataaca taaaaaatta
     7021 gttatacaat atatctatat gtatctaatt ttgcattgat aaaaacaatt atggataaaa
     7081 ggtaaagcat gatagaatct aatgtagagg tttgtatttt aatggaaaaa tttagttttt
     7141 attgcttaag atacaaattg aattacgaag agaagtaatt gggaggaata ttttcatgac
     7201 aaatttgcca aaacaatttg atagcttcaa tgaagctaga caaaaaggat ttataaatgc
     7261 aaaggaactt aaggaaagtg ggaaaaagat ggttggagtt ttctgtacat ttactccagt
     7321 tgaaattcca atggcagctg gagcaactgt agtaggtgta tgcggcgtta gtgaagagcc
     7381 aatacctgat gcagaaaggg tgcttccaag aaatctttgt cctttaatta aatcaagcta
     7441 tggacatgca ataacagata cctgtcctta tttctatttt tctgatttgt tgataggaga
     7501 aaccacttgt gatggtaaga aaaaaatgta tgaagaactt gcaaaagtta agccaacata
     7561 tgtaatgcat ttgccaaata cttcaaaagg agaatttgcc tataaacttt ggaaagatga
     7621 aatgataaga ttaaaagaag aggttgagaa atctttaggt gtaactataa ctgaagaaga
     7681 tataagaact gctataaaag ataaaaatga agaaagagaa cttttaaaag agttttatgc
     7741 acttggtaaa ttacaaccat ctgcacttac aggccttgag ttacataatg ttttatatca
     7801 agcaggtttt aaatttgacc gtgcagaact taaacattct ttaagaaaag ttatagatga
     7861 tatgaaagaa agatatgaaa aaggtgaatg tcctgttcaa aaagataagc caagaatatt
     7921 gataacaggt tctcctattg gtggaataag cgaaaaaata gttaaaactt tagaagatgc
     7981 aggggcatca gtcgttgcat atgaactttg tggagctatt agatcaaacg atttattggt
     8041 tgatgaagaa atagaagatg tatatgatgc tttaacacaa aaatatataa atataggttg
     8101 ttcttgtatg atgaataatg acaatcgtat tgaattatta gatagaataa tagatgaata
     8161 taatgtagat gctgtaatag atgtagtttt acaagcatgt catacattta atatagaaag
     8221 ttatagaata agagaatttg ttacaaaaga aaaaaataag ccatttatgt ctcttgaaac
     8281 tgattattca aaatcagata ctgaacaatt aagaactaga tttgaagcgt ttgtagagat
     8341 gttgtaggaa taaataaaag taggaggaac tatgtttagt ataggagttg actcagggtc
     8401 agtggcaaca aagggtgtgc tatttgatgg tgaaaaaatt attaagaaaa taattatacc
     8461 aactggatgg agccctaaaa gtacatctaa acaggtctat gagttgctaa gtagtgaaat
     8521 tgacaaaaaa gatataaaaa aggtggtagg tacaggttat ggaaggggag taatggattt
     8581 tgctgataag aaagtaacag aaataacttg tcatactaga ggtatttact ttttaaacaa
     8641 gaacataagg actattttag atgtaggtgg tcaagatagt aaagttataa atttagatag
     8701 agatggaaat gtatttaact ttataatgaa tgataagtgt gctgcaggaa ctggtcgatt
     8761 tttagagatt acttctaacc ttttaggaag cgatattgaa agtatagata cattagctaa
     8821 aggatatgaa ccagttaata tatctagtat gtgtacagtt tttgctgagt ctgaaattgt
     8881 aagtcttcta gctcaaaaca tttctactgg agaggtagca gcagggatac ttaaatctat
     8941 tgcaaataaa tccacctcaa tgttagcaag aggtgaggta atagatgaag ttgcatttac
     9001 tggagggctt gcaaaaagta aagaactagt aaaaatgata gaagaaatac tgggtaaaaa
     9061 aatttttatt gcagaagata ctcaaataat aggagcttta ggagctgctg taataggatt
     9121 tagataaaaa catataaaag gaaaatgaga agctgaattt attataaata aattcagttt
     9181 tttattttat tttatttatt tataaaggaa aactttggta taattgattt aaatgattgt
     9241 atatttgtaa atacttgata tttagtcata tataacatta taattttaaa aattagctca
     9301 aaataagggg gagaaagcgt gaaaaaattt acttcaaaaa aagtaacaag gtttgttgcg
     9361 ctgtttttaa tatctatttt agtaatgtca acaattcctg tatcagctga caatagcaat
     9421 acattgaatc agataaaaaa agcagaatac agtgagacgt ataaacaata tctaaatgat
     9481 gctaaaaatg gacgtacaga aaaatataat gggattatac caaatccata tcagttggaa
     9541 ggtactcaaa tccaaagtaa aggaagaaca gctccaacat cttatgaccc tagaaaatta
     9601 ggtcttatga catcaataaa aaatcaagag gatttaggaa tctgttggga ttttgctgca
     9661 atggcaacct tagaaagttt cttaaagtta aataattatg gagactatga tttatcagaa
     9721 gaacacttaa gatggtgggc ttcagatggt gaatatggat ggtctgtaaa tgatatgaat
     9781 ggtgctttaa attatgaagc tatgggatat ttaacatctt ggtcaggtcc taaactagaa
     9841 aaagatattc catacaatgg tagagtatct aaagcacaag gagcaaaaaa accaactaat
     9901 atgaatacag cacctactgt ttttaatgtg acagatgctg tgtgtgtaag taatgacata
     9961 aattctacaa aaaatgctat attacagtat ggagcagtaa catctggtta ttatgaagat
    10021 attaaatatc aaagtgatga ccaaaattca tattattgtc caacaaaaag ttttaataca
    10081 aatcatgcta tttcaattgt aggttgggat gataactatt ctaaagataa ttttaatgca
    10141 aatattagac catcaaaaaa tggtgcttgg ttaataaaaa atagctgggg agattataat
    10201 tctgagggtg gatatttctg gatatcttat gaagacaaga cacttatgtc tgatatagat
    10261 aatttttcaa tcaaaggtgg taaaaaacca aatgatgatg agaagatgta tcaacatgac
    10321 tatgcaagta ttgtaccttt aatttcaaaa aagataacag ctgcaaatgt atttgatttc
    10381 aatagaggcg atgaaactct aaaatctgta atgttcttaa ctgaaagtat aggagctaag
    10441 tatgaagtat attatgctcc agtagtaaat ggtatacctc aagaaaacaa tatgaaaaaa
    10501 ttaaaagaag gtactgccca atattcagga tacataacag ttccaattga ttcttttgat
    10561 ataccagaag gaaagggtgc aattgtagtc agtatagagg ggaaaaatgg agagtcaact
    10621 ataggttctg agtcaaatgt accaggatat gatacattta aagcaaaagc taatttaggt
    10681 gaaagttata tcatagataa tactggaaaa ttctttgata taaatagaga tagtaacttc
    10741 tatccgtgta attttactat aaaagctgtt acagaaaagt cttctggtga gagtatccca
    10801 aatgagtctt taattggtag tgatagatat gaaactgcta tcaaagttag tcaaaatggt
    10861 ttcaattctt cagaaaatgt agttttagta aatggcagtt caatagtaga tgcattggca
    10921 gcaactcctt ttacatctgc tataaattca ccaatattat tgacacaaaa agaagcttta
    10981 aattcaaaaa ctaaagctga gatacagaga ttaggtgcta agaaagtata cctaataggt
    11041 ggagaaaatt ctataagtaa agaaattgaa caacaattaa aaagcttaaa tatatcgata
    11101 gagagaatat caggaagtga tagatacaag acaagtttat tattagctca aaagttaaat
    11161 ggtataaaaa atgtatctca aatagcagtt gtaaatggag tgaaaggatt agctgatgca
    11221 attagtgtag gcgctgcagc agcagaaaat aacataccaa tcatacttgc aaatgaaaag
    11281 agcgaattgc aaggagctga tgaatttcta aattcattaa atatagagaa atcttatatt
    11341 attggaggaa cagcatccct atcaaacaat ctagaaagta agttaaaaaa tccaactaga
    11401 ttatctggta gtagtagaga cgaaactaat tcaaagataa tagataattt ttataaaaaa
    11461 gatactttaa aaaatgcatt tgtagttaaa aatggaatca agaatcaaaa tgatttaata
    11521 gatggattgg cagttggtcc tttaggagca aaaactgggt caccagtaat tttagttggt
    11581 gataaattag ctgatagcca aaaagaagtt cttaaaaata agactttaga aaaagtaact
    11641 caagttggtg gaggagcaaa taaaaatgca tttaaagagt tagtaaaatt aaaaagtgct
    11701 aaatagagta cttaagtatt aacatatatt agtgtgtatt agtaacttat cttaataaaa
    11761 gctgaaaata attacttcaa attattgata aataagataa tcaatgattg taaagttttg
    11821 ttttcagctt taaatttatg tagtataagt ttaaaaacat aaaaaagggt tactttatat
    11881 agaaatatag aaaatcttac attttatata taaataaccc atatttaatt aactttttat
    11941 agtgttctaa tagatattat tgaacttaat ggtaaatata atgtgtaagt ttcatttaaa
    12001 gttggattag attcatctat atatcttgag caaagtgtaa ttgataagct atctgcttct
    12061 ataatttcac catatagagg acaactgcta aagtaagaat gtacttcaac atatttattt
    12121 aagttttctt gaacataatc gtaaaaacgg ttttttgtag tagtagccat aaaatcaact
    12181 cctttatttt ttattaaagt tgaaactttt aaaaagataa atatgattac ttctattaga
    12241 aactaagtta aaaaataagc aaatagatat gaaaattaga ataatatatt atttatatct
    12301 taattataac actagataat aaaaaatgct atataaaatt cagaattttc gaaagttttt
    12361 aagcttattt ttaaatatac taataatact attgacatta gatagaaaaa gtattatatt
    12421 gactatataa attattttag tcaaactgga gggaaaagat gcctaaatcg tattctgata
    12481 aagaaagaga gtatataata aaaagactta aagaagaagc aagattgtgt atggagcaat
    12541 atggtattag aaaaactact gttgatgagc ttgtaaaacg ggtaaaaata ccaaaaggaa
    12601 ctttttatct attttttcaa tcaaaagaat tgctcttttt tgaggtatta agagatatac
    12661 atgactctat acaaaaagaa atcttatatg agataaatag agtagatgaa agtattacat
    12721 gtgaacaatt gacagatata tttatgaaat tctataggat ggttgatagt acttcaattt
    12781 taaatcttat gataaatgga gaatttgaga tattggtgag aaagttacca gattctataa
    12841 ttgaagaaca ctttagacat gatgactttg aaattggaga gattatatct tgcataccaa
    12901 atgcaaaaaa taaagacata gaaagcttta gtggagcatt tagagcagtt tttcttacca
    12961 tgctatataa gcgcgaggtt ggaagtaatt gttttgaaga tgctttaaga ttaatgataa
    13021 acggattagt tatacaactt atggaataga atttaaattt tgtttgctaa taacttttat
    13081 taaaaattat taacaaacaa aaaaattata ttttattgac catatagtca aaatagtcaa
    13141 aaatttgaaa gggtgatatt gaatgattaa agttgatgat ttatcattta gttatacaga
    13201 gagagatttt ttacaaaata ttaactttgg agtaggaaaa ggggaaatac taggattttt
    13261 aggaccatca ggtgcaggaa aatctacatt acaaaagatt ctaattggaa tgataactaa
    13321 ttatggagga agtgtcattg taaatggagt agagagtaaa agacactcaa ataaatttta
    13381 tgaaaatata ggagttgatt ttgaatttcc tagtttatat gaaaagttga ctgcaataga
    13441 aaatttaaaa tattttgggt ctctatattc taaaaaacta ctatctattg atgagttatt
    13501 aaaatcagta ggactagaaa atgaagcaaa taaaagggta tctgagtatt caaaaggtat
    13561 gaaatctcga ttaaatttta taaaagcttt attacataat ccagatattt tatttttaga
    13621 tgaaccaaca agtggtcttg acccatcaaa tagcaaggtt atgaaagata taattttatc
    13681 agaaaaatct aaaggtaaaa ctattatttt aacaactcat aacatgttag atgcaacaga
    13741 gctttgtgac agagtagcgt tcattgtaaa tggtaaaata tctgctttag atacaccaca
    13801 taatcttatt atgtcaaaag gagctattaa agttagatat acttactttg ataatggaga
    13861 aaaaacttct gaatgttttc tgaacaatac tgctaatgat aaaaatttga atatgcttat
    13921 tgagaaaaat aaacttttat caatacatag tagtgaacca acattaaatg atatttttat
    13981 tgagataaca gggaggaatt tacaatgagg ctaaaaaatc ttattttagg agatataaag
    14041 tttcaattta agtatggttt ttatttttta tatctttttt taagtattat atatatttgt
    14101 attatcaatg tatttccaac ttttatgaga gaaaaaatag ctataattat gatttattct
    14161 gacccagcag caatgggatt gttttttatg ggagctattg tacttcttga gaaaagtcaa
    14221 agggttttaa attccttagc tgtatctcca gtaaaagtat ctgagtatat tttatcaaag
    14281 gtaatttcat taggagtaat atctagtatt gtagctatgt ttattgctat tacattaaat
    14341 ttagataata ttataatatc aactataggt accttttttt cttctattat attttctttg
    14401 cttggattga tacttgcaag taaagcatct agtttgaatc agtttattgt tttaagtatt
    14461 cctattgaga ttatatgctt tattccacca atactcaatg tacttcttga cacaaaatca
    14521 tatgcaaatt tgtacccttt taatatatgt atttctctta ttagtggaga taaaaatttt
    14581 ataatgatta atattttaat tttaatatca attataataa taatttattt tataacttat
    14641 tattttattt gtagcagttg gaaaaaggta ggtggagtaa aactatgata aagattataa
    14701 atgcgtttaa acaagagttt gaacaaatta agagagatgc tatgcttttt atagtatgtg
    14761 tttctccaat attatgtgga gtatttataa aatttggaat accattaata cagaatatat
    14821 cacttaacaa gttttattat caattaaatt tagagccgta tttcttgatg tttgatttac
    14881 ttttagcttt tattacacct tttatgttct tttttgcttc tacaatggtc atattgggag
    14941 aaattgatga cagcatttca agatatttaa tagtaactcc gcttggtaaa actggttatc
    15001 ttatttcacg atttggaata ccaggtatct tagcatttat tattactatg attctactta
    15061 tctttttttc tctaactaaa atttctttct tgttaaactt atcaatttct ttattagttt
    15121 tattacaagg aattattata tcaatattag tgatttcttt atcatcaaat aaactagaag
    15181 gaatggtaat tacgaaattc tcaggtgttt ttatgatggg tattttagca ccatttttta
    15241 ttttaaataa agcacaatat attctgttct tcttaccaac tttttggata tcgaaggcat
    15301 ttaaggaaga taattatgtc tatatgttta tttcaattgt tatatcatta atatggattt
    15361 tattattatt taaaaagttt agtaaaaaaa tagttaaata agaaaattta atatataaat
    15421 gtaaggttga cttgacagta atgttgaaaa atgttacatt taagatgtaa agttaacctt
    15481 acatgaatgg agcaaatatg aaaaataaaa taaagatatt aagagaaaaa ttaggattga
    15541 cacaagaaca actagggaga ttagttggaa catctagaca ggctataaat gctatagaaa
    15601 ctggtaaaaa tgaaccttca atatggttgg catatgatat ttcaagaatt tttaatgaac
    15661 caattgaaag tgtattttta tttgaagaaa gtgaaagaaa atcaagagca cagataagta
    15721 ggggagaata ttatggctat aagaaagata agaacatttg atgatgaaat tttaagaaaa
    15781 aagagtaagt atgtagaaaa cgtagataat aagataagag aaatattaaa tgacatggca
    15841 gagaccatgt acaatactcc aaatggagga ggattagctg catgccaagt aggagtatta
    15901 aagcgattag ttgttataga tttaggagaa ggacttatta agcttgttaa tcctgaaatt
    15961 attaaacaag aaggggaaca aattgttgtt gaaggatgtt taagttttcc tgaagtatgg
    16021 ggtaaattaa aaagaccaaa aaaagttaca gtgcaagctc taaacgaata cggtgagaaa
    16081 atagaaatta aaggttcagg ttttatggca aaatgtcttt gtcatgagat agaccattta
    16141 aatggtatag tttttacaga taaaattatt gaacatgtaa aattgtaaga tagattggtt
    16201 attgaattta taaaatattt tatagtaagg agttaaatta tgagattatg gcataaggat
    16261 ttaattgatg tattacctaa aaatcaatta gtaagtcaat ggagagagct acttgctata
    16321 aaaggttcta tagataaaaa aggtactcca aaccatctat tagtgaataa agtattgaat
    16381 tatagtatag atgaatttaa attttataca aaaattgtac atgatgaaat gttaaaaaga
    16441 aattataaac ctaatgaact taaatataca agtatattaa aatggaaaaa tagaaatttt
    16501 gcaaatgata tatctaatga acatagttta aatttagaaa atttatatga tgattggcat
    16561 aataaaatgt atttaaagca atgcttatat aatttagaag aaaaagcaac atgtggagga
    16621 ataccaataa atgaatggaa tatattactt tgtaaatata gtaaagatta tgaattgtgg
    16681 tctggaaata tcatgtttta attactatag attaacatta tagtattatt gtatataaag
    16741 aggtgattat ttgtataata aaataaaata tattccaata aattgtgaaa tagcttgtaa
    16801 taaattaaaa aggagtatgc catataaatg ggatttaaat atttatcgtg gatgtgagca
    16861 tggatgtaaa tattgttatg ctatttactc tcataagtac atcaactcca ataattattt
    16921 tgaagagatt tatgtaaaaa caaatatagt agaaaaactt gagagacaac ttaaaagcag
    16981 taaatggaag aaagaagtaa tcaatatagg aggagttaca gatagttatc aaccaataga
    17041 agctgactat aaaattatgc cagaaatatt aaacctgcta ataaaataca agacaccagc
    17101 tattatttct acaaagtcag atttaatttt aagagattac gatttaattg ataagctatc
    17161 taggattaca tatatcaatg tagcatctac aattaccaca gtaaatgaag atacacaaag
    17221 gcttattgag ccaaatggtg tggattcaat gagacgattt aaaatgttaa aagaatttag
    17281 aaaaactaac gcatcagttg gtctacatat tatgccaatt ataccatata ttacagatga
    17341 ttttgataat ataaattcgc tatttagaca tgcaaaagaa tgtaatgtac attatgtttt
    17401 acctgggact ttatacttaa ggggtataac tagaggtgtt ttttttgact ttgttaaaaa
    17461 agagtttcct gaattatttg ataaattatc aatactatat agtgaaggtt cacttaatag
    17521 ggagtataaa aatcaattgt ataaaatggt taatgaactt agatataaat attctctatc
    17581 aagcagttac tctaaaataa tgaaggaaaa gttaaaaaac tcaggagatg tacagatatc
    17641 tttttttgat tagttttata taaaaaaatt ttttttacta tgatgattaa aatttattaa
    17701 aattaaaatt tattgacaaa tataaaattt aaatatataa ttaattcata aaataaaaat
    17761 atgaaaggat gatgttgttc ggtgatattt gatatcataa aatcgttgat ataagtattg
    17821 ctggaaaaga gtgaataaaa tcaaatttaa taagcaaaca aagtaaagct atagttttat
    17881 tgttgtgctt aaaaatattt ggttctattt tactcttatt taaactcatc tttttgaagt
    17941 taaaccacag agtaaactct gtggtttttt tattttaaaa ataaatttta aggagtggta
    18001 tgttttgtta aacaaattat gtatgtattt agaaagacca gagctttata aacaaagtga
    18061 aattaatttt tgggatgatg agtatatttc agagcaatta ttaaaagcac acttagacac
    18121 taattttgaa ggagcaagta gaaattttaa ctttatcgag gattctgtaa attggatagt
    18181 tacagttgca aatccagtta attatcctaa attacttgat ttaggttgtg gtccaggttt
    18241 atatgcagag aaatttgcac aaaaaggtta taaggtaact ggaattgatt tctcaaaacg
    18301 ttctattaac tatgcacaaa atagaaataa ggagactaat ttaaatataa attacttgtt
    18361 tcaaagttat ttaaatatga actataatga ggaatttgat ttggctacct taatttattg
    18421 tgattatgga gctttgtcaa ctgaaaatag aaggttatta atggaaaaaa tttatgatag
    18481 tttaaaacct ggaggaaaat taatattaga tgtatttaca ataaataaat acaataattt
    18541 tgaggaaatt aaatattggg aaataaatga agatggagga ttttggagta atgaaaaata
    18601 tatgtgtcta caagataatt gtaaatacaa tgattataat acattagaac aaactcttgt
    18661 aataaccgaa aaagatgaaa atgtatttta tgtttggaat cactattttt caaaagaaag
    18721 tttcttatta gaagttgaaa atattggatt taagtctgtt gagtttttta gtaatgttaa
    18781 gggtgaagca tattctgacg atagtatgac tatgggatta gttttacaaa aataataatt
    18841 tttttaaaat gaataaataa aaacttgaaa cctttaacaa taaaataaca taagaaaagc
    18901 ttacatattg aaaaatggaa acttttatgt tatttttagt acattacaaa tttgtttata
    18961 taaaaatcaa tacttgaatt attattttta tgtgaaattt atctatagta tatttttata
    19021 aacaagtaat gttattaaaa ttaagtatat agttaaaagt gtatagcaaa aaggctgtta
    19081 tattaatgtt agaggttcag ggaaaatata taaagcaaag atatttacag ataatgtaga
    19141 ttgcacagct ataagttaga taatagaatt atgtaatcaa gaatttacaa caaagtataa
    19201 gataaggata atgctaaatt gccatacagg tgctagatgt gtaaatggat ttaattaata
    19261 ctattgataa agtgcaacaa gcacataaac caattgatga gattattgaa actataagag
    19321 atatagttga cataattaaa atattggaac ctgtttataa ttttaaatca aatcaataat
    19381 gattaagatt aaaataatat taaggcatca atgttataag agtacaatat aaggagtact
    19441 atatagtact ccttacttat acatattagg caatagggaa tttttttata gttaaatttt
    19501 caaatatttt ttagggaact ttataattga gaattaaggg tatagtatgt gatgaactta
    19561 ctaaatttat aattaggtta tataatggaa taatcatcaa tagttttact ttagggaaaa
    19621 tatatgctta aggaagtata gcttactaat attattaaaa tgtatattta aaatgataaa
    19681 aaaagaatta gacaagtatt tagtaaattg actatgcaaa attctatcaa gcaggaacac
    19741 tatgacttta gtcatgggag gttcagtagg taacttgttg aattaagttt atattattta
    19801 aataaactac ttaatttaga taatataaaa agtcagaatt ttaagaatag agctatataa
    19861 tcttagctat taggagaagt actatgatag gaatatattt tagtggaaca ggaaattcaa
    19921 gatactgtgt agaaaaattt ttacaagaat atgatataac agcaaattct tattctattg
    19981 aagataatga acttttacag cacatcaata atcatgagaa tattattttt agttatcctg
    20041 tgcaatatag taatgtgcca aaaatattga aagattttat aataaatcat tcaactttgt
    20101 ggaatggtaa acgtgttttt attattgcaa ctatggctct atttagtgga gatggctctg
    20161 gtgtgttagc tagaatatta aaaaaatacg gtgctataac tgttggtgga ttacatgtga
    20221 agatgccaga tagtatagga gatgaaaagg tattaaaaca ttctttagag tataatagaa
    20281 agcttgtaat aaaagcagaa aagaaagtaa ctaaagctgc taaaaaactg aaacatgggt
    20341 caccaccaaa agaagggttg ggttttttga gtcatattgt agggttattt ggacaacgat
    20401 tgtattttat caataaaaca aatatgtata cagataaact aaaaattaat actgaggaat
    20461 gtattgtatg tggtaaatgc gttaatttat gtccaatgaa gaatctagta attaaaaatt
    20521 gtatgattgt tgcaaatggg caatgtacaa tgtgttatcg ttgtgtaaat aattgtgcca
    20581 aaaaagcaat tacattgtta ggtaaaaaag ttgttgaaca gagtaatatt aaaaaatata
    20641 tgtaagaaat ttaaagtttt attgaatgta aattgatgta tattttatgt tagaaagaga
    20701 ggttaaataa tggaatacta catagttgat tcatttgcaa ctaaactatt taaaggaaat
    20761 cctgctggag tttgtgttct tgacagaaga atacctttag agttaatgca gaaaatagca
    20821 gaagaaaaca atttaccaga aacagcattt gtagttaaat gtaaaggaaa ttatgaatta
    20881 cgatggttta ctcctaaagc agaaattgat ttatgtggtc atgcaacttt agcagcagct
    20941 tatgtaatat caaattttat tgatgttaat gtaaaaagga tagatttctt tactcaaagt
    21001 ggaaagcttg aagttacacg aaatggaaat ctatatgaaa tgatatttcc agaaataatg
    21061 cctattgaaa tagagttatc accacaacaa gctaatttga taggctgtgt accaagtgct
    21121 gtttattctt caagagattt aatcttattg ctaaacagtg aacaagaagt tataaattat
    21181 aaacctaatt acgcacaatt gcgtaaattg acagactggt taggtataat aatcacagca
    21241 caaggaagta atacagattt tgtatcaaga tatttttgtc cagaattaga ttcagaagac
    21301 cctgttactg gttcatcaca ttgtaatctt attccatact ggtctgaaaa gttgggaaaa
    21361 cacaaaatgg tagctgctca actttctaat cgtggtggta taattcagtg tgaagtgttg
    21421 aaggataata ctgtaaaaat ttctggtgaa gcagtcttgt ttatgcaggg aacaataaag
    21481 attgatattt aaataagtat aataactaat ttattatgaa aaataattaa ttttcttagt
    21541 gttaacaata ataaagtaga gttaaaaagc ttgatttcac ttaaatcaat atgatagatg
    21601 ttgatactaa gattgacaag catgattaaa aaagataata tttaatctat aaagataaaa
    21661 acacagtctg gttggtagtc cagacgcagc aatagctgtc agtaacctgc ctccttggtt
    21721 gtccgttctt tagaaagtaa tttaaggagg aactagcaat ggacaaaatt agtggaaaat
    21781 taacagtttt atttgaagaa ccattttggg taggtatatt tgaaagacaa gatggtaaaa
    21841 aatatgaagc atgtagggta gtttttggag cagaacctaa agaagtagaa gtgtatgaat
    21901 ttatacttga aagatttttc tcattagatt ttggtagcat taaattagaa aaaaatgtga
    21961 ccaaagacaa cattggatac aaaagaatgc agagaaaagt aaaaaaggaa caagaaaaag
    22021 aaactatagg gactaaggct caaaatgctc ttaagcttca atatgaagaa agaaaacagg
    22081 atagaaaaaa cctagctaaa aatagaaaag aagaagaaaa agaaagacta tttaatttga
    22141 aacaagaaaa gagaaaagct aaacataagg gtcattaatt ttaattaaat atctttttaa
    22201 taattgctag aaaaaggtgt ataaaatttt taattttata caccttaatt tattgaaaag
    22261 aataaggttt tatatttcaa ttactttaaa atataaaaat atactaataa aaattgtaaa
    22321 aatagtataa atggtatacc aaatgtaaat ttaatgtgtt tagttttatg tctaaaagaa
    22381 tacattccaa ttatagaacc tatactacct ccaattactg ctatagacat aagagtagct
    22441 tcttttattc tccattcgtt tctaattgct cttttcttat ctatgtacat tgaaaagaaa
    22501 gcaatagaat taatcactat aaaatatatt agaataaaat ttttcacagt cttgcctcca
    22561 taaaaatata ttatacatta taaaacaaga tatttgtttt ggaaagttaa atttgtaatt
    22621 agtacatata aaatatttga taagaacaaa tataatatta attggaaaat ttaaaaaaat
    22681 taaacaatta gcaaataggg ggtatattta taagacacaa agtgataaaa tctatttaga
    22741 aaataatgta aaataacagg aggataaagc aaaatgaaat ttatagtaga aaaagaagtt
    22801 tttgataaat tagaaaatgt atgctttggt gtagtagttg caaaaggaat agataatact
    22861 aaagaaatag aaagaataag caacctttta gatataagta tagatagagt agaagattac
    22921 ttcaaagata aaaaagtaaa ggaatcagaa gaaattatac catatagaga ggcatttaga
    22981 agtctaggca tgaacccaaa taaatttatg agttcgatag aagcaatgac aacaagggtt
    23041 gctaagaata agaaacttcc tcatataaat cctattgtag atttaggaaa ctctatttct
    23101 ttaaaatatt tacttccaat gggtgctcat gatatggatt ttagaaatga tgatgtatat
    23161 gttagatttt ctaaaaaagg ggataaattt gtaccatttg gagaaactga tgttgaatta
    23221 atggaagaag gagaattaat ttattcagtt ggagacatgg taaaaactag aagatggata
    23281 tggagacaag gagaggaagg aaaaattact aatagctcaa aaaatatatt ttttcctata
    23341 gatggtttta ctgatgctaa tttagataag gtaatgagtg caagagaaga attagcaaaa
    23401 ttgttaaagg aaatatttaa ttgtgaaatt aaagtaggtt ttgtagataa agataatcct
    23461 gaaatggaaa tataataagt tgttgaatac ttgttaagat attatattta cttaatgatg
    23521 ttaaaaaggt attaaaaaag aaaggtcgta gtaaatttga aaaaaactat aataaagaat
    23581 gtaaatccaa tgattgaaaa taagtttata ggactttttg aaatagagta taaaaacaaa
    23641 ttagatgaag ataaggtttg gatggtagca tcaagaaaaa gtagtgagca attaaaaaat
    23701 atttatttga agaatgaaga agatagtgta gatgcagttg taatagttgg gcttcataaa
    23761 agtagtaaaa aattaatttt aataagacaa tttagagtac ctattaatgg atatatatac
    23821 gagttaccag caggtcttgt tgatgaagga gagtctatag atatttccgt tgaaagagaa
    23881 cttagagaag aaacaggact tactctttta gaaattaaaa aaaataaaag tattgataaa
    23941 gtttatttat ctcctggaat gagtgatgaa tctgttgcct ttgtttattg tatatgtgat
    24001 ggtgatataa cagatgaatt tttagaacca gatgaagaga ttgaagcgct tttaatttcg
    24061 caagaagatg caaaagagat tttacaaagt aatcataaga tagacacaaa agcatttttg
    24121 attttacaaa tgtttgtgag tttaggagct aaattatttg aatagatata tagaaaagag
    24181 gttatataga agagtaaaat aataaagttt attttacttg tataaattat aatctctttt
    24241 tttatacaat aaagtataat tttgtgtatt tttaaataag tgacttgact cttaaatttt
    24301 cataatatgt tataaaccaa tttaagatac tacaataatt aaaattccaa aattatttaa
    24361 catatttgca attaaaatga gaaaaggtaa aggttgaatg attgaaaaat ttgatgtaaa
    24421 atagtagctt agtttaatat tatagtttgt agggttaaaa attagaaatt gtaagggaga
    24481 taaattctat gaaaaaatta gttattattt taattagtat cattagtatg gttagcttga
    24541 cagcttgtaa aaaaagcaat gaaagtaaat ataataattt aaacaacaaa gaatccggac
    24601 aaacaaccat tgaaatggac ttagataaaa attatgatac ttcagaccca tttgttaatg
    24661 cacgtttgtt ttgtgtgtct aatgatatag atgttttaga cacagagatt tcttttaaaa
    24721 tggatggaga tagtggtatt gtggaaataa aggacaataa aacatatgaa actttatgga
    24781 gtaatacatg gcatggaagc gttgataatg atacattcac aatttctctt tctaatatac
    24841 aaaaagagaa agagtatgct atatgcttta ctggaattaa aataaatcat gcagttgtaa
    24901 aggttacttt tgaaagcaat ttagttaagg agaaagaaaa accctcaaaa taaattccag
    24961 attgtctata acttgatatt cagtacataa aagcagttga tatttatgat tgactgcttt
    25021 ttcttatcaa aaaggagagt ttgtgcattt ttacttgaaa tagtttatgc acatcaaaag
    25081 ttggtgagca tgatattgga aatggtaagc atgaaatctt gataagacaa ggaaataaat
    25141 ttaagttttc aattaagtat atttccataa tttagttaac aataagctaa ttattattta
    25201 tattttgata tataaataat aattgtagca aatttcaaaa aatcatatat tttttgaact
    25261 aaataaaaat ttatttttat tattttaaat tagaggagat tcttgaagat atattatttt
    25321 taaaaataag tatagtttaa aaattcaaaa aaaagatggc aaaatatgtc ccaagggaca
    25381 ttgagtaaaa taaatgattt cagcttattt acactaaaaa gtacatacta aatgaggggt
    25441 aaaataaaga aaaatgatat actaattgtc ttttgataat aaaaaaacat ggaatttgat
    25501 tatttaatca agaaaaagta ttgaacaaat gatttattta gtgtacaata atcatgtaaa
    25561 aacatagtaa tggagggaga catttcataa tgaaaattaa ggtaggaatg aacggatttg
    25621 gaagaatagg aagagctgta ttaagaatag ctcaagaaga attaggagat aatatagaaa
    25681 ttgtagccat aaatgcaaga gcaacaacag aatctttggc acatttattt acatatgatt
    25741 catgctatgg aacttttaga ggtgaagtag aggcaaagga tgaagacact ctagtagtaa
    25801 actctaaaga gataaaaata ttaagatata atgacccaga agaattacca tggaaagaac
    25861 tgggggttga tatagtaata gaatcaacag gattatttac acaaagagaa aaggcagaaa
    25921 agcatattaa agcaggagca aaaaaagtaa ttataacagc tccaggaaaa aatgaagata
    25981 taactatagt tatcggagtt aatgagaaag aatatgataa tgaaaagcac aatataattt
    26041 caaatgcatc ttgtactaca aattgtctag ctccttttgc taaagtattg gacgaaaaat
    26101 ttggtatagt aaaagggtta atgactacag tacattcata tactaacgat caaagaatat
    26161 tagataaaag ccataaagat ttgagaagag caagagcagc ggctgaatct ataataccta
    26221 ccactacagg agcagcaaaa gctgtgtcaa gagtactacc acaattagaa ggaaaattaa
    26281 atggtttttc acttagagta ccaacaccaa cagtttcact tgttgattta gtgtgcgaac
    26341 ttaaagaaaa cgttacatct gagcaagtta actctgtgtt aaaagatgct gctgaaggag
    26401 aattaaaagg tgttttagga tattgtgaca agccacttgt ttctatagat tataaaggag
    26461 attcaagatc atctattata gacgctctat ccacaatggt tatagaagac aatatggtta
    26521 aggtagtttc ttggtatgat aatgaatggg gatattcttc aagaactgtt gacttagtaa
    26581 aatatgtagc tgaaaaatta aaataatata tatttcttaa agcaaatttt aaaaaaattt
    26641 tttaaaaaaa ttatataaaa ctaaaaaaaa agattatgaa aaatattgaa ggaaaaattt
    26701 tttcataatc ttttttcttt catgcaaaaa gatacaagtt acaacttata tttgcatatt
    26761 aggtcgttag agaagtatat atatatttag taaggaatca tactttttat aaaatgaaag
    26821 ataagatgtc gaaaacttgc ataaatctga aaaattttta ttattaaaat actgatttcc
    26881 attttaaaaa tgataaaaaa atgacaaata aagttgcaaa tagtaatact tataagtata
    26941 attaaaaaag agaaaaggtt ttgttaaact ttataacaaa aaaagaaggg atgattcaaa
    27001 atggagaagt taacagacag gagacaggtt aaggcagtaa gcatggaaac cttcattttc
    27061 ctaatattac tagtagttgg ttttgggtat gttggtagta ttatgggagc aggaatgatg
    27121 ttcaaagtaa taatgagtac agcacatgct ttactattgg agacagtttt cttaattatg
    27181 gctatggcag ttcttgcagg cgcattaagc gcactacttt cagagtttgg agtaatagcg
    27241 ttaattaata aaattttcgc tgtatttatg aagcctttat atggactacc tggtgctagt
    27301 atagcaggag ctataacaac ttatttatca gataatccag ctattatacc ttttgctaaa
    27361 gataaaacat tcacacagta ttttaaacaa taccaagttc cagcattatg taacttagga
    27421 acagcctttg gtatgggact tatcttaaca acatttatga tttctcaagg tacagaatat
    27481 gtacttccag cattaattgg taatttaggt gcaattatag gtagtgtcat aagtgttaga
    27541 ataatgttga catttactaa gaagttttat aaatacagtc cggaagaaga taaagcaaca
    27601 ggaacattag agaaaaagga tgaatttaga gaaatcagag aaggaaatgt atttcaaaga
    27661 gctttagatg caattcttga aggtggtaaa atgggggtag atatgggaat ggctataatt
    27721 cctggagtcc tagtagtatg tacattggtg atgttattaa cttttggacc atctacagac
    27781 cctgttacag gtcaagaagt ttatacagga gctgcatatg aaggaataaa attacttcca
    27841 gttattggag ataaacttgg atttatatta gaaccattat ttggatttac atcaccagag
    27901 gcaatagcat tccctataac ttcattggga gctgttggcg cagcaatgtc tctagttcca
    27961 gagtttataa agtcaggagc tataactcct aatgatatag cagtatttac agctatgggt
    28021 atgtgttgga gtggatattt aagtacacat attggtatga tggatgcatt aaatgctaga
    28081 cagttggcag gtaaagctat tctttctcat acaataggtg gtttatgtgc aggagcagct
    28141 gctcacttta tatttacact cgtaggataa atgtaatcag ctaagataaa aaataatata
    28201 caaaaaatgg ttgttgatat gtaataagaa tttacatatc aacaaccatt tttttctgac
    28261 taaagataat ttataataca taaacattaa gtggatatta gagttaagtt atatttatgt
    28321 aaaaagtaac aaaaatctgt agaaatttat gacaaaattt agtacactaa ctatatgttc
    28381 ttatttaaaa tttaaggtta gaattaggaa ttacctagta gagtaagctt tgattaataa
    28441 aagaatcata aaataaaaat attagtcata aagattataa ataacttact taaaataaag
    28501 ccatagaact tacaaaaatt aaaaaagcat ataaatatgg aggggtacaa tggatgaatt
    28561 atttcatata ggaattgatg taggttctac aacggtgaag gttgtggtac ttaataatag
    28621 taataatata gttcataaag aatatagaag acattattct gatgtaaaaa aatcagttaa
    28681 agaagtacta aatggtatat atgaaaaatt gggagatata aatactacca ttataataac
    28741 tggttcaggt ggaattggaa tatcaaaaaa attaggtgta aaatttgttc aggaagttat
    28801 atctagtaca aaatcaatag agtattttca tcctgagact gatgttgtca tagaacttgg
    28861 gggtgaagat gcaaagataa catatctaag tggaggaata gaccaaagaa tgaatggaat
    28921 atgtgcaggt ggcactggag cattcataga ccagatggca tcactcttaa agacagatgc
    28981 cagtggatta aatgaacttg ctaaagggta taatgttata tatcccatag cttcaagatg
    29041 tggtgttttt gctaaaacag atattcaacc tcttataaat gatggagcca atcagactga
    29101 tatagcaatg agtattttca atgcagttgt cgtacaaact gtgagtgtcc tttcttgtgg
    29161 aagaaaaata gaaggaaatg ttgcgttttt gggtggtcca ttgtactttt tatctgaact
    29221 tagagaagca tttaaaagag tattagattt aaaagatgaa aacataatct tcccagaaaa
    29281 tgcacaactt tatattgcta ttggagcagg attattatca gtagatgaag ataatataca
    29341 actaaaatct ctaatagaaa aattggatag tataaagaat attgaagatg gagaagtaaa
    29401 tttattagaa cctttattta aagataaaaa tgagtatgaa gaattttcaa gaagacatga
    29461 aaaagaaaaa ataaattatg tggacataaa ttcaataaaa agcaattgtt atttaggaat
    29521 tgatgcaggc tcaactacaa ctaaagcagc acttattgat gaggatggaa gacttgtata
    29581 ttcttattat aatagcaatg aaggaaatcc attaaaaaca accattaaag ttataaatga
    29641 agtatatgat atattaccaa aaaatattaa aatattgagt tcaactgtaa ctggatatgg
    29701 agaaggtctt attaaaaaag ctcttaaaat cgataatgga gagatagaga ctatagcaca
    29761 ttacaaagct gctaaatttt tcaacaaaga tgtagatttt atacttgata ttggtggtca
    29821 agatatgaag tgcctaaaaa taaaagatgg agtaattgat aatataatat taaatgaggc
    29881 ttgttcatca ggttgtggct catttttaga gacatttgca agttctttat ctatgacaat
    29941 agaagaattt gcgcatgaag gtatttattc tcaaaatcct gtagatttag gttcaagatg
    30001 tactgtattt atgaattcta gagtaaaaca agctcaaaaa gaaggtgcaa gtgttggaga
    30061 tatatcagca gggctatctt attcagtaat aaaaaatgct ttatttaaag taataaagat
    30121 tagagattta aatgagattg gtaataatat tgttgtacaa ggtggaacct tttataatga
    30181 tttggtgctt agaagctttg aaaaactaat tggaaaaaat gtaattagac ctaatatttc
    30241 aggaattatg ggagcttttg gttcagcact tatagctaaa gaaaaatatg aaagtggata
    30301 caaaacatcc ttgttatcta aggaagagtt gaataatatc aagctagaag caagtgtaac
    30361 tagatgtaaa ggttgttcaa atcattgtct tcttacaata aataaatttt ctgataatga
    30421 aatttttatt tcaggtaata gatgtgagaa gggtgaagct atttatggtg ataaaaaaat
    30481 aggattagaa aagaaaaaac caataaattt atttaagtac aaatacaata gaatttttag
    30541 atacaaacca ttggaaaaag aagaagcaaa aaatggggaa atagggatac caagagtatt
    30601 aaatatgtat gaagattatc cattttggtt tacttttttc aatgctatgg gctttagagt
    30661 tgtactttca gataggtcat caaaacagtt atatgaaact ggtataacta gtattgcatc
    30721 tgaaacagtt tgttatccag gaaaattagt tcatggacat atagaaaatt taattgcaaa
    30781 aggtataaaa aatatatttt atccaagtgt aacaaatgaa aataaagagg attcaaatgc
    30841 agataattat tataattgtc ctgttgtaat atcatattct gaagtaataa aaaacaatgt
    30901 agagaatata agaaataaga acataaatta tataaatcca tttataagtc taaatgataa
    30961 ggaaaaattg aagaaaagac tctatgatga attatcaaga catttttctg atataaaaat
    31021 aactaaagaa gaagttaatc atgcagttga taaagctact gatgaacaga atttatttaa
    31081 aatggagata caagcagcag gtgaaagagc acttaaagaa ataaaagaaa gaaatatgaa
    31141 ggggattgtg ttatgtggaa gaccatatca tatagaccca gaaataaatc atggaatgcc
    31201 agagcttata aattcattag atatggcagt gcttactgaa gactctatat gtcatttagc
    31261 caatgtacaa agaccactta gagttgtaga ccaatgggta tatcattcaa gattgtataa
    31321 agcagctagt tttgtaagag ataaaaacta tttggaactt gttcaactta attcatttgg
    31381 ctgtggatta gatgcagtaa ctacagacca ggttcaagaa atacttaatg aaaaatcgaa
    31441 gatatatact attataaaga ttgacgaagg caacaattta ggagcagcta agataaggat
    31501 gaggtcctta aaagcagcta tgtttgagag agaaagcaaa aatatagatg taaaaaatat
    31561 aaaggtaaac aggatacagt atgctaaaaa taatcagata acgaacaaac atactatatt
    31621 agcaccacaa atgtctccaa tacatttcca attccttgaa aaagctgtta acttaagtgg
    31681 ttataatata gaaattctaa aagatactga tagtagtgta attgaagaag ggttaagata
    31741 tgtaaataat gatgcatgtt atccagctat aatagttgta ggtcagctaa taacagcatt
    31801 aaaatctggc aaatatgatt taaataacac ttctgttact ataactcaga ctggtggagg
    31861 ttgtagagct acaaattata ttggattctt aagaaaggct atatacgatg ctggatttaa
    31921 agatgttcct gtcattgcac ttagtgtaaa tggaatagaa gatagtggta taatggataa
    31981 tatatcacta aaattaatca atagactctt tatgtctgtt gtttatggtg atttgctgat
    32041 gaaagtacta tatagagtta gaccatatga aaaagttact ggaagtgcaa atgctctata
    32101 tgaaaaatgg gttgatatat gcaaatcttc tcttgttaaa gcaaaaattt ctgtatttac
    32161 tagaaatata aaagatatag taaaagaatt tgataattta gagatactag atatcaaaaa
    32221 accaaaagta ggtttggttg gagaaatatt ggtgaaattc catccaattg caaataataa
    32281 tttagtagat atattagagc gagaaggtgc tgaagcagtc gtgccagacc ttacaaattt
    32341 cttcttaagt tgtgccttta atactatata caaacacact catttagaag gaagtagaaa
    32401 gagtagaatg ataggagagg catttatata cataactgga atatatcaaa gagtatataa
    32461 aaaagcactt gataagagtg agagatttta tgctccagcc aatataaaaa atgttgctaa
    32521 aagtacagaa ccagtagtgt cattaggtaa tcaaactgga gaaggttggc ttttaacagg
    32581 tgaaatggtt gaattattaa atgaaggtgt agaaaacatt atttgtatgc aaccttttgg
    32641 atgtttacct aatcacataa ttggtaaggg ttctataaaa gaattaaaga ggttgtataa
    32701 aaatgcaaat ataataccta ttgattatga cccttctgca agtgaggtaa atcaacttaa
    32761 caggataaaa cttatgcttt ctaaggcttt taaaaatata taagatttat attttgatat
    32821 gttaaaagtt aatgaattta aaaatatact ctatattaag tatctataaa aaagagttat
    32881 cctatttttt agggtaactc tttattagtt aaaaaattat atgtatactg atattaaagg
    32941 ggggaagtaa tatgagttta gtatatgatg tttataaatc aaaattagga aatttgtaca
    33001 tattaagtga agatggtttt atagttagta tatacatagg agatgaaaaa tttaataatt
    33061 taaaaaatga atgtattaaa aaaggaattg atataaagag aagtaaggaa gatttaaagg
    33121 aagcaataag ccagatagat gattacttta atggaaaaag aaaagtcttt aatttaaata
    33181 ttcgtattga agggactgaa tttcaaaaat cagtttatag ggaaatgtta aaaatacctt
    33241 atggagaaac attgtgttat tcagatatag caagaaatat aaacaatcca aaatcagtaa
    33301 gagcaatagg acaagcaagt aaaagtaata aaattccaat aataatacct tgtcatagag
    33361 tagttggcaa aaaagatatt ggtggatata tggggaatca ttctgatttg aaagagatac
    33421 tattaaattt agaaagacag agttaactta gaaagaaatg tttttattgt taatatttga
    33481 tataataata gtaaaaagaa gtatttttta caaaaacaat gaagaattta ttttaattta
    33541 caaaaatgag ggggaatttt aatgtgtgat aaagttatag catcaaaata tcatgagaag
    33601 ggatttaatt gtgcagaatc agttataaaa gcatataatg aggaatttaa tacagatata
    33661 ccagtttgtt taggaagtgg actgggttca gggtgtgggg ttgctagttt atgtggtgca
    33721 gtaaatgcat caaatataat aattggatat gtaaaaggca gaaaccacga agatgaaagt
    33781 actaaagcta aagtatatgc taaagattta actacaacag taagaaaaga gtatggttca
    33841 gagttatgta tagacctaaa aaaagattta gttgcttgta gagaaataat ggatttcgca
    33901 tatgatagtc taaaggaaac tttaaaaaaa gaattataag aaaataagct atcaaatata
    33961 aattatttga tagctta
//
LOCUS       DAYASV010000029        33548 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYASV010000029 DAYASV010000000
VERSION     DAYASV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.029"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            141..983
                     /locus_tag="ACGI68_001418"
     CDS             141..983
                     /locus_tag="ACGI68_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HFH0137862.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            998..1462
                     /locus_tag="ACGI68_001419"
     CDS             998..1462
                     /locus_tag="ACGI68_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137863.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            1476..2621
                     /locus_tag="ACGI68_001420"
     CDS             1476..2621
                     /locus_tag="ACGI68_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137864.1"
                     /translation="MNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPELAFEEYKSQ
                     KALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPGMGHACGHNI
                     IGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFNNVDAALILH
                     PSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSVNSMRLHLKD
                     YARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKGAAISTGTNV
                     EIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGNLTHEIPAIQ
                     AYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            2665..3600
                     /locus_tag="ACGI68_001421"
     CDS             2665..3600
                     /locus_tag="ACGI68_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HFH0137865.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(3874..6567)
                     /locus_tag="ACGI68_001422"
     CDS             complement(3874..6567)
                     /locus_tag="ACGI68_001422"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFH0137866.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDGGGNNGGGSTTPPDGGGTPAT"
     gene            6781..7479
                     /gene="yunB"
                     /locus_tag="ACGI68_001423"
     CDS             6781..7479
                     /gene="yunB"
                     /locus_tag="ACGI68_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HFH0137867.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(7691..8698)
                     /locus_tag="ACGI68_001424"
     CDS             complement(7691..8698)
                     /locus_tag="ACGI68_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HFH0137868.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            8926..9810
                     /locus_tag="ACGI68_001425"
     CDS             8926..9810
                     /locus_tag="ACGI68_001425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0137869.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            10012..11562
                     /locus_tag="ACGI68_001426"
     CDS             10012..11562
                     /locus_tag="ACGI68_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0137870.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            11591..12238
                     /locus_tag="ACGI68_001427"
     CDS             11591..12238
                     /locus_tag="ACGI68_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFH0137871.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(12380..12640)
                     /locus_tag="ACGI68_001428"
     CDS             complement(12380..12640)
                     /locus_tag="ACGI68_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HFH0137872.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFRYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            12857..14140
                     /gene="hflX"
                     /locus_tag="ACGI68_001429"
     CDS             12857..14140
                     /gene="hflX"
                     /locus_tag="ACGI68_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFH0137873.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYANLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDADLDEEDFNIYREYILEK"
     gene            14148..14825
                     /locus_tag="ACGI68_001430"
     CDS             14148..14825
                     /locus_tag="ACGI68_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFH0137874.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            14911..15354
                     /locus_tag="ACGI68_001431"
     CDS             14911..15354
                     /locus_tag="ACGI68_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFH0137875.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            15659..16303
                     /locus_tag="ACGI68_001432"
     CDS             15659..16303
                     /locus_tag="ACGI68_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFH0137876.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(16389..16766)
                     /locus_tag="ACGI68_001433"
     CDS             complement(16389..16766)
                     /locus_tag="ACGI68_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFH0137877.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(16766..17152)
                     /locus_tag="ACGI68_001434"
     CDS             complement(16766..17152)
                     /locus_tag="ACGI68_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFH0137878.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            17331..18086
                     /gene="nadE"
                     /locus_tag="ACGI68_001435"
     CDS             17331..18086
                     /gene="nadE"
                     /locus_tag="ACGI68_001435"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFH0137879.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            18169..18888
                     /locus_tag="ACGI68_001436"
     CDS             18169..18888
                     /locus_tag="ACGI68_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0137880.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(19055..20059)
                     /locus_tag="ACGI68_001437"
     CDS             complement(19055..20059)
                     /locus_tag="ACGI68_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0137881.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            20325..21221
                     /locus_tag="ACGI68_001438"
     CDS             20325..21221
                     /locus_tag="ACGI68_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HFH0137882.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREINLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            21342..22604
                     /locus_tag="ACGI68_001439"
     CDS             21342..22604
                     /locus_tag="ACGI68_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFH0137883.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            22680..23873
                     /locus_tag="ACGI68_001440"
     CDS             22680..23873
                     /locus_tag="ACGI68_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFH0137884.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            24502..25296
                     /locus_tag="ACGI68_001441"
     CDS             24502..25296
                     /locus_tag="ACGI68_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HFH0137885.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDVAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            25391..26731
                     /locus_tag="ACGI68_001442"
     CDS             25391..26731
                     /locus_tag="ACGI68_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0137886.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            26868..28100
                     /locus_tag="ACGI68_001443"
     CDS             26868..28100
                     /locus_tag="ACGI68_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HFH0137887.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     TLIESGIVTMNKEACEIC"
     gene            28140..29282
                     /gene="acrC"
                     /locus_tag="ACGI68_001444"
     CDS             28140..29282
                     /gene="acrC"
                     /locus_tag="ACGI68_001444"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0043958 - acryloyl-CoA reductase (NADH)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HFH0137888.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            29293..30087
                     /locus_tag="ACGI68_001445"
     CDS             29293..30087
                     /locus_tag="ACGI68_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFH0137889.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            30101..31120
                     /locus_tag="ACGI68_001446"
     CDS             30101..31120
                     /locus_tag="ACGI68_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0137890.1"
                     /translation="MRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLAD
                     QLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERKK
                     PEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMATI
                     VCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSVN
                     LTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGTT
                     VRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEMI
                     ESLNR"
     gene            31219..32595
                     /locus_tag="ACGI68_001447"
     CDS             31219..32595
                     /locus_tag="ACGI68_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFH0137891.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(32927..33271)
                     /locus_tag="ACGI68_001448"
     CDS             complement(32927..33271)
                     /locus_tag="ACGI68_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137892.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        12440 a         4108 c         6149 g        10851 t
ORIGIN      
        1 atattttttt gattcataac taaaagtata taaaaatgat gaataaatat atattataca
       61 ttatcaatga taggttatat aataaaatca ctaacaatta tattataaga aaattataaa
      121 aattacaagg aggtatagaa atgaaagaaa aagcagggat tagaaatgcc ataatagcag
      181 cctttataat cacaatattg gcagaattaa ttggacctat ttcatttaaa gttatgggaa
      241 ttaatgtaac tttacttagt atactatggg ctatattcat aggaatggct gtttctcctc
      301 atttattagg gagagttata ccagccttaa aaaagtttat aggggataat gaaataaatg
      361 tatctccata tttattatct ttgacactat atccattagg gataatgttt ggaattaatg
      421 cagggcctaa agttggtata ttacttcaag caggaccagc acttttatta caagaatttg
      481 gtcatatggg tacaatgttg atagcattac ctgttggagt agcattaggt cttggaagaa
      541 gtgcattagg tggtactttt tcattatgtc gtgacacagc actaggaatt attggagata
      601 agtatggatt aaactcacca gaaggtatag gaacactagg aacttacata agtggtagta
      661 tatttggaac attattattt tcatttttag caccagtggg tgtactttta ggatttcatc
      721 catatgcttt agcaatggca tcaggaatgg gtagtggttc aatgatggga gctgcaacag
      781 catcattaat gaatacagtt ccacaaatgt cagagcagat tttagcatat tcagctacta
      841 gtggattgat tacagctgtt actggaatat atatagaatt attcttatct ttaccagtag
      901 ccaactacta ttatagcaaa gtagctccag ttattgaaag atttagagga agaaaaacaa
      961 gaagaacaag taataaattg taaaggagga tatatatatg gaaaaaactt ttaagactat
     1021 tatggagcag ataaaaatag tattgtttat atatattatt atttgctttg gacaaatgat
     1081 agcacttaag actcaggctc aagatttgtt gattggaagt gtatctggat ttttgttggt
     1141 gataattgct attataataa agaattttgt taaaaaacct aatttaccag gttttgcatg
     1201 ggctacatta gttgcatttg ttttatcgct tccaatatct ccagtggggg atatcatagc
     1261 aacaaatgta gggaaaatca attttatgtc tacagtaaca ccattattag cttttgcagg
     1321 aatctctgta ggtaatcaat tagatatatt aaagactttg agttggaagt tagtagttat
     1381 atcatttata gttatgacat ctacttactt tggttctgct tttatatctc aaacagtttt
     1441 aagattaaat ggaatgcttt aaggaggtag taaatatgaa tgaattaaaa gcaaaaatta
     1501 aagaggaaat aaagttatta tctgaagaga aaaagacatc atttgaaaaa gtttctgact
     1561 atttattttc aaatccagaa ttagcatttg aggagtataa gtcacaaaaa gcattatgtg
     1621 atttgttaga agaaaatgaa tttaatgtaa ctaaaggtgt aggaggatta gaaacatctt
     1681 ttgaagctgt gtattcaaat ggaactaatg ggaaaacagt tgctttttta gctgaatatg
     1741 atgcacttcc aggaatggga catgcatgtg ggcacaatat catagggaca agttctgttg
     1801 gtgcaggtat catattaaaa gaaatcatga aaaaacataa tatagaaggg actgtaaagg
     1861 tttttggaac acctgcagaa gaaagagtag gtggaaaaat aactatgatt aaagaaggtg
     1921 tatttaataa tgtagatgct gcattaattt tacatccaag tgatgcttca atgcctgatg
     1981 atatatcatt tgctcaagtt aatttaaaat ttgattttac tggtaaggca tcacatgctg
     2041 ctgcttttcc ttgggaaggg aaaagtgcct taagtggagt aatagcacta tttaacagtg
     2101 taaatagcat gagattacat cttaaagatt atgcaagagt acatggaatt ataactgatg
     2161 gaggtagcat acataatata attcctgaaa aatctacagc tatatttaat gtaagagcct
     2221 taagtataga atatttaaat gagatttgtg aaatgcttaa aaattgtgct aaaggagctg
     2281 caatttctac tggaactaat gtagaaatag ttcaattaga tgaaatatac aaagagatta
     2341 agaatgattc tgaattagtg aatatagtta gacaaaattt tgaagttttg ggtgaagact
     2401 atgttgaaag agatttaagt caaggtatag gttcaactga tacaggaaat ttaactcatg
     2461 agatacctgc aatacaagca tatataaaat taaaagaaaa tactgcaact catacagatg
     2521 aatttgctgt tgcagctggt ggtgaagaag gcagagtagc acttataaaa gcaattaagg
     2581 tgttagcaat gtgtggagta gatattttgt atagtaaata atatataatt ttaaaatata
     2641 ttaagaaagg aaatcggatt agacatgata ttaaaacaag taatagaggt ttatgacatt
     2701 ttagataaag caaatgctaa tggggaagaa gtaaaaactt acttacaagg ttatggtgaa
     2761 gtagatgtaa ctgtaaaaga attgtcttca agtaaaggtt caacagattt agtaaagctt
     2821 acaattcctg gaaaaaatgg aaaattaaaa ggtggagatg ctcctactct tggtatttta
     2881 ggtagactag gagggattgg agcaagacca gaagttatag gttttgtctc agatggtgat
     2941 ggagcattgg tagcaattgc agtggctgct aaacttttgg atatgcaacg aaaaggggat
     3001 atattagatg gtgatgttgt aatttcaact catatttgcc cagatgctcc aactaaagaa
     3061 cactatccta ctccttttat ggattctcca attgatatga tgactatgaa tgaaaatgaa
     3121 gttgatagta gttgtgatgc aatactttct atagatacta ctaaaggaaa tagagtaatc
     3181 aatactagag gatttgcaat atcgccaact gttaaagaag gctacatatt aaaaactagt
     3241 gataaccttt tagatattat gcagactgta acagggaaat ctccatttgt attccctttg
     3301 tctattcaag atataacacc atatggaaat aatctgtatc acttaaatag tattttacaa
     3361 ccagcagtag ctacagatgc accaatagta ggagtagcaa ttacaacaga gatgcctgtt
     3421 gctggatgtg ctacaggtgc aactaatttt agtgatttag attcagctgg gagatttgca
     3481 attgaagttg ccaagctatt tggtagaaat aagtgtgatt tctatgataa agaagagtgg
     3541 gaaatgctta ttaagagata tggcaaatta aatagatttc aaacatttgg tatacagtaa
     3601 ttagaaaaat attaagttta tataatttgt gatataacta tttaagaata tataaacaat
     3661 taggaataaa agtaagaata aagcacttag ttaaaaactt taaaaataaa atatttattt
     3721 gtttatataa aaaggaggat tctggttatt gggattctct tttttatgaa tacaaataat
     3781 atattgtaat tataaaaaaa ggagcgtttc aaagtgattt ttttaatcac cttgagacaa
     3841 ctccttatat ttttatttaa attctatttt ttattaagtt gctggagttc caccaccatc
     3901 aggaggagta gtagagccac caccattatt tccaccacca tcaggaggag tagtagagcc
     3961 accaccattg tttccgccat tatcaggagg agtagtagag ccaccaccat tatttccgcc
     4021 attatcagga ggagtagtag agccaccacc attgtttccg ccattatcag gaggtgtaga
     4081 accgccacca ttatcacctg tgttattatt gttattatta ttttcttctg gttggttatt
     4141 gtctggtttt tttgtatctt cagatgatgg ttgagaactt actttagtag gagcagtacc
     4201 ttcaataaat gctgctttag agccagaaga ttgctttcct ccatcctcta aattaatttt
     4261 tgtaaaatat acatttttag gaacttcaaa ttcagtaacc gttaaattag catgtatttt
     4321 ttccataatt ttttcccata gttttgcagt actagtactt ccatgtggta cttctctacg
     4381 ttttatagtg ttaccagaat catcttttct accagcatca tctccaatat aagttgcacc
     4441 aacataataa ggagtgtaac cgacaaacca agcatcatat gcactatttg tagtacctgt
     4501 tttacctgct acaggcattc cctttggaag tgtagcaaca ccaccagtac cttcagtaat
     4561 tacgctttct aacatatctg taacaacata agctacttca gggtctacaa ctttatgttc
     4621 ttcaggagca tttttaacta ataattgacc atcaaaggta gttatagtag taaaaataga
     4681 aggttctaca taaataccgt gatttgccaa tgtaccataa gcagcagcca tttgaagtgg
     4741 agtaatacca ttcgccatac cacctagtgt caatgatggg aattttctat cacttgcttc
     4801 agatgaacct gagtgattat tctttaaagt tgttatacca aagttcttta agtaatccat
     4861 cattatatct acacattcat cataagagtc tccaagcatt tcagcagttt tgacagctat
     4921 cgtgtttgaa gatttagcaa gtgctttacg taaactcata gaaccatgac cagcagtagt
     4981 agttcttgga ttccatttat tattttcttc aaacttatat cctcctcgag aatcacttag
     5041 agcagttgct tgtgttattt ttagagtatc aatagctggc gtatatattg ctaaaggttt
     5101 tatagaagaa cctggctgat gagggttagt agcacgattt aaagtttttc taccacttat
     5161 atttcttcct ccagctagtg ctcttatttc accattttta taatctaaga ttaccattgc
     5221 agcttgcggt tggctaactc catcaggacc gaccatatgt cctgggaaat tgctatttct
     5281 atcaaattct tcttctaaag catcttgcat tttagggtct atagttgtgt ttacaattag
     5341 accaccattg tagaataagt tttgagcttc atcatttgta tgtccttgtt ccattaaagc
     5401 atttataact tcactttcga taaggtcttc tacagatgaa gaaactttat ctgatgattg
     5461 aggcaatttt atttctattt tagctttgat tgcttcatca tattcacttt gttttatata
     5521 tttcaattct aacattttct ttaaaacaat ttcttgtctt tttttagctt caggattact
     5581 tacagccttt ctaacaacta atgtacctgc ttttaattgt ctataagtat cattagatat
     5641 aagctcccaa gattttattt tttcaatcat atcaaagtcc acttgagtag ggtcatctaa
     5701 atcatcatca gttgtgttaa caaaaaataa taatttattc tctaaatcct ccttagtttc
     5761 atttccctct aacttagaag ttttataggc tgaaaatctt gatggatttt tagttgaacc
     5821 agcaagaagt gctgactctg ctagagtaag gtcagctgca gacttatcaa aataaccacg
     5881 tgcaccagcc tcagcacctg ctagacctct accaacgaag aagttattta aataagtttc
     5941 tagtatttta tctttactta ctgttttact catctcatgt gcatagtaaa tatcctttat
     6001 tttacgaggt atagtttgct gttcagttgt taacagcatt ttggatacct gcataggaat
     6061 agtactacca ccttgcttag tacctgtaag ggtctttaat actgacctaa ataaaccttt
     6121 aatatcgaca cccttatgct cataaaaacg ctcatcttct attgcaatta cagcatgttg
     6181 aagataaggg gaaatcttat ctaatggaac gggtgtcttt ttatttacac ttggagctgt
     6241 agataatgtt tttccattag cgtatttaat agtagttgtt tggttcgtct gtttatctaa
     6301 aacagcttct gtaactggag ttacatctct tagtgacgca aatactagac cagcagtacc
     6361 tgctgtacca acaactagta aaactaaaaa tactatccca aatactctta gttttttaaa
     6421 tttatcgctt tttttagatt ttttcttgtt ttttgttgaa cgagtcttgt tagctgaagt
     6481 tctattaatt ggtttatttg tattaccaga tgagctaact ttttttctcc tgattttatt
     6541 tccattttta tcattatcgt tattcataag attttaacct ccttacttaa tgcaatatta
     6601 taacacaaat aggagtagtt ttgtaattaa tagaaaaaat gaatttgcta taatattctg
     6661 tacattgtat atgtataaac gtaatatatt aaaaagtcta tatttttatt ttatttattt
     6721 ttgacaaata ttataaaaat tatgccaaag ctacatataa ataaatagga ggggataatt
     6781 ttgaatagaa tagtagatag aaaaacaaaa aatgaattta gaaaaatcat agctattttt
     6841 ttagtaattc ttttcttgtc tgtatttata ggcagtttta tatatataga taaaacttta
     6901 agaccgacaa taacagtttt agcagagact aaagctcttg aactagcaaa taggtcgata
     6961 aataaagctg ttgctgagat ggtagaagga aaaataaatt atgaagattt aatggatata
     7021 caacttgata ataatggtaa aataactatg atacaagcaa acactataat gatgaatgaa
     7081 attgcatctg caatagcttt agaaatacaa gatgaattaa aaaaggataa gacagcaagt
     7141 tcttacatac ctataggaac agcattagga agtcctatat tagctaaata cggtcctaaa
     7201 cttgaagtat ctattgaacc tataggaaca gtatcagtaa actttaaaac ggaatttgag
     7261 tcttcaggta taaatcaaac tagacataga atatatttag aagctcaaac acaagttaaa
     7321 gtagtaatac cacttataac ttcaacaaaa caaattaaag ctcaaatacc tatttgtgaa
     7381 actataatag taggtgatgt tccagaaagt tatgtaaata ttccagaaaa aaatctagga
     7441 aatgttttac ctaacactgg aaagaatacc aacaagtaat gtaaagttgt atatttttaa
     7501 ataaattcaa aataatagtt tataaataaa atttttggaa ataatattaa tataatttta
     7561 ataaaaattt atattttata ctatgtaaaa tatctaatat atttggtctg taggttaaat
     7621 aaaagtacct ttagaccaaa tatattatta ataacttaat aacatcttaa aacttattta
     7681 tttatattat ttacaacatc catttaataa atacaccata acctacagct gggtcgtttt
     7741 caattgcatg tgacactgct ccgattattt tattatcttg aactattgga gtaccactca
     7801 taccttgtac aataccacca gtttgagcta aaagcttttt atctgtaatt ttaattttaa
     7861 aacttttact ttctggatgt ttttggtttt ctatatctat aatttcaata ttatattttt
     7921 tacacttatt aaagttatct tgtaaaatta tttgagcttt tcctaattta acttcatcta
     7981 aagatgctac tttatatttt tttaacttgg aaatatcaaa gttattaatt tgacctctta
     8041 ttccaaaatc agtattttct gtaaagctac caataatatt ttctcgtttt gcatttatac
     8101 atccaacact acctttataa gatttttcta tattaaggtg cttagttgta gaaattgaac
     8161 ctgttttaat aggtattttt cttgcattac caacacttat tggatgacct acagctccaa
     8221 actctgatgt atctggattg atatacgtta aagtagcaaa tccagaaagt aaattgttaa
     8281 aactaatttt ttctaatatg tgtttggtag ttttcattgt tataagttgt ccacctctat
     8341 ttaataaaat agatacttgt ttttcttctg gtaaagcatt taaggtattg gcaaagtcac
     8401 tatatccatc aacttcagca ttatttacct ttactatagc atcacccaat ctaaatggag
     8461 atgcttctcc aggatttcta actatgatat tttttaattc tgcgtcaatt tgaagtacgt
     8521 ttccaattgg tattaaatat tcaggttcat ttttttgtaa attttgtgca taaattaaat
     8581 tatttgagaa aaaatataat aaaaataaaa atgttaaaac aattgtaaat tttgacttag
     8641 ctaagttggt ttttttactt aaagtattat ttttaaaatg aaaattttta agttgcataa
     8701 aacacctctt tcttattaaa ttattttaat aatagaattg aagttttctt gctgcttcta
     8761 tattatgttg tgcaagaatc atgttttgat gcttggaaaa ctagctcaat aacaccttag
     8821 ttttcataaa aataaataaa ataaatagtg tatatgataa aataaataca tattcatata
     8881 aataaaaata aacaataact gtctagcata agaaaggagc aaaaaatgaa aaaatataga
     8941 ttgttagtta cttgtataat tgccatggga atcataatta taggaacatc aaatccagtt
     9001 agcaattttt acaagactac aaatttgatt agttcaggaa atatcacaaa tgaaaaagca
     9061 gataaagatg aaaattcaaa caaaaataat aaagataaaa atgaagacaa atctaattct
     9121 aaaagtaata aagataagtc atcagaggac aaatcgtcag ataataaaaa tgaaattcaa
     9181 aaaaataaaa aacttttaat ttgtatagac ccagggcatc aaggaaaagg agatagcaat
     9241 ttagaaccag tagctccagg ttcttcctct aaaaaagcga gagtatcttc aggaacagag
     9301 ggtattgcta caaaaaagcc agaatatgtt ttaaacttag aagcatctct tgttttaaag
     9361 agtatattag aatctaaagg ttataatgta ataatgacta gagagacaca tgatgtaaat
     9421 ataagtaatt cagaaagagc aatacttgct aatgacaaaa aagcagatat ggttgtaaga
     9481 atacatgcag atagtttaaa taattcttca aaaactggag catctatatt gattcctgaa
     9541 aaagatggaa aatatacagc tcctatatat gaagaaagta ataaatgtgc tgaatttatc
     9601 aaacagaata tggaacaatc aggtatacaa atcaatggaa tcgttcaaag aggtgatttg
     9661 actggattca actggtcaaa agtaccagct gtattagtag aaatgggatt tatgagtaac
     9721 tataatgaag accaaatgat gtcaaatcca gactaccaaa gaaaaatgat gcagtgtata
     9781 gctgatggat tggatgcgta ttttaaataa gatatgaata gagaaaaagt taggttggta
     9841 ttatttgcct aatttttatc tctatttttt attgtacata acacaaagaa gaagtttatt
     9901 ttaggttaaa ttgtcgtaaa tataactaaa aatattggga atacttgatt taagtttaaa
     9961 aagaatatat tatagtatag aatgtgtaaa atttttttga gaggtgttga aatgctagta
    10021 gtagaaaatg tgagtcatgg atttggtgcg agaacaatat tagaaaatgt atcctttaga
    10081 cttagaaaag gtgaacatat tgcactagtt ggtgctaatg gagaaggaaa atcgagtttt
    10141 ttaaatataa taacaaagaa gcttatgcca gatgctggaa atataaaatg gtcttcaaga
    10201 gctacagtag gttacttaga ccaacatacg gtattaagca agggaaaaac tataagagaa
    10261 gtgcttagag aggcttttaa gcatatgttt gacttagagc aagaaatgat tgctatgtat
    10321 gacaaaatgg gagaagctag tgatgatgaa atgagtaagc ttcttgaaga aacagcagag
    10381 atacaaacta tacttgaaaa tagtggtttt tatatgatag atgcaaagat acaagaagtt
    10441 gcaaatggtc ttggtcttgg agaaattggt cttgataaag atgtaactga cttaagtgga
    10501 gggcaaagaa caaaagtact tcttactaaa cttctacttg aaaatccaac tattcttata
    10561 ttggatgagc ctacaaacta tttagacgaa gagcatataa catggcttac taagtacctt
    10621 caagagtatg agaatagttt tgtcttagtt tctcatgata ttgaatttat aaataataca
    10681 tgtaatgtaa tatatcatat ggaaaatgga gagttaaata gatataaagg aaattatgat
    10741 gaatttgtaa gacttaatga tataaagaaa cgtcaagaag aacaagctta tgataaacaa
    10801 gttgaagaaa gaaaaagact agaggatttt gtagctagaa ataaagctag agtagcaact
    10861 agaggaatgg ctaacagtag gcaaaagcaa cttgataaga tggaaatatt agaaagacca
    10921 aaagaaaaaa taaaacctac ttttgctttt aaagatgcta gagcagctag taaaataatt
    10981 tttgaaacag aaaatttagt tttagggtat gatgaagcat tgacaaaacc acttaatttc
    11041 catttagaaa gaggaaaaaa gattgcctta aaaggaatga atggtattgg aaaatctacc
    11101 ctattaaaaa ctttacttgg aataataaaa ccatttgaag gaaatgttaa attaggtgac
    11161 tatttggaag ttggatattt tgaacaagag agttcaagag aaaatagtaa tacacctatg
    11221 gatgaagttt ggagtgaatt tccagggctt acaaactttg aagttagaca ggctcttgca
    11281 aaatgtggac ttacaaatga acatataacg agtcaaatga gagtattaag tggtggagaa
    11341 gctgctaagg ttagactttg caaagtaatg ttaaaaaata taaatttctt agtattagat
    11401 gagcctacaa atcatttaga tgttgaggca aaagatgaat tgaagaaggc tatcaaagag
    11461 tttaagggaa cagttctttt agtatgtcat gagccagaat tttattcaga aattgttgat
    11521 gatgtatgga atatagaaga ttttacaact aaaatcgtgt agtgatttaa cttattgctg
    11581 gggggatttc atgaaaaagt ttctaaatgt acttttttca atttgtatta gtattgttat
    11641 aatagtaggt gttattaatt ttactgttgg atttaaacaa ctttattatt ttgacattga
    11701 ttacttaaat atatcagagt tatctggttt atctaaagat gatataaaat tgaactacga
    11761 ttatctaatt gattataact taaataagaa tgtgtctgag ttcaaattac caacattaaa
    11821 atcttctcca caagggaaga tacattttga agaagtcagg aatatatttc aaaatataaa
    11881 taaattagct aaattattat tggtagtttc tttagtagga attatattga gtgtaaaaaa
    11941 taaaaatata aaaatcttaa aaactacatc aataacactt ataattatgc cattattatt
    12001 aacagttcca attttattaa attttgaaaa gtcatttatt atttttcata aattattatt
    12061 tagaaatgat tattggatat ttaatcccga tttagaccca gttataaata tattgccaga
    12121 ggaatttttt tttcactctg gaatgatgat tttaatatta attttgttag taagtatagt
    12181 actgtttgta atgtataaat tatgtaaatc tttatcaata aaaatatttc aatgttaaat
    12241 agagggatat ttttatttcc aaaataagat aataaaaaag gattagatta aaagttatac
    12301 aataaaaata gctgtgcaat aatatttaga ctatgaaagt agttgaaaaa tatttatgca
    12361 cagctattta taatagaatt tactttttac tagtgatttt aactaaatct tgaaatttac
    12421 tcataatgta attgtaatta tcttttttat gaggtaatat acaactactg caatctttga
    12481 tacctttatc tgtataccta aagtttccac cacagttttc tcctaaagca tacaaaggac
    12541 aatagcaaaa tagacagtta aactcttctg gtttattagt tttatgacaa gggaaaaatt
    12601 cgcaatcttt gtgattaaaa aacttataat tttcgcccat attttcctcc taattaaata
    12661 aaatttaaat tttttatact atataagatt ttggtgataa tataattata ctaaataatt
    12721 ttatgaatag tcgataatat agtattatta atcatagtat taatggtata attagtcaac
    12781 agaaaaataa tgtaaaaata atgtaagtgt attttgtaat tagaatatgt tttataacaa
    12841 aaaatgaggt gattaagtga aagaaattca agaaaaagca cttcttgtag gtctgaattt
    12901 gactacaata gtgaaaaaaa atgatgatat agacactaat gaatctatgg aagaattaaa
    12961 agaactaaca aaagctgctg gggcagaagt tgtaggtagc ttaattcaaa ataaacactc
    13021 tgtagatgca gcttattata taggaaaagg taaagtagaa gaaataagag cctatagtga
    13081 ctctttagat gctactttag taatttttaa tgatgaatta tctggtgctc aaatcagaaa
    13141 tatagagaat gttgttggaa gaaaagtaat agatagaact actttaattt tagatatatt
    13201 tgctcaaagg gcacttagca aagaaggtaa attacaagtt gaattagcac aattaaaata
    13261 tagattgcca cgactttatg gaatgggtgg tgagatgagt agaacaggtg cagggattgg
    13321 tactagaggt cctggagaac aaaaactaga aatagataag agacatatac ttaataaagc
    13381 agcagatata agacgtgaac ttaaagaagt aaaaaagaat agagagacac agagagttaa
    13441 gcgtttaaag tcaaatatac cgattgtagc acttgttggg tatacaaatg caggaaaatc
    13501 taccctgttg aacgaattaa taaagacaca caaggattat gaacaagaaa aagaagtttt
    13561 tgtaaaagat atgctttttg caactttaga tgtaactttg agaaaggctc ttttgccgaa
    13621 taaaaaagaa tttttagtag tagatactgt tggatttgta agtaaacttc cccatgattt
    13681 agtagaagcg tttaaggcaa cattggaaga agtacagtat gcaaatttaa tcttacatgt
    13741 aattgatgca acaaatacta gttatgaatt gcaaaaaagt acgactgaag gtgtattaaa
    13801 agaacttggt gtaaatgata aaaaacatat acttgtatat aataaagtgg acaagctaga
    13861 attggatata taccctaaaa gtcaagaaga tattgtgtat atttctgcaa aacaaggcat
    13921 aaatatggat aagttattaa atatgataga aattgcttta atggaaaata cttattcagt
    13981 aagtttgatg ttaccttatg agagaggtga tatatttagt agaataaaag ataaatataa
    14041 tgtggagaat tttgagtatg gggaaagtgg tataacgcta gatgcagatt tagatgaaga
    14101 agattttaat atctatagag agtacatact agaaaaatag gttgaatatg gatataaact
    14161 gggaaaatat agataatctt gaagattatt ttataaccta tcttctttat aaagaatcta
    14221 aaacagtatc tcaaattagt aaaattagaa atatttcaag tactgaagca agtgagcaac
    14281 ttatacaagc aaagttaaaa ataaaggaaa tgcaaaaaga tgattttgag gcatctaagg
    14341 atattttgga taaatttcta gaactagata aaattaagag acttgatttt atggatagct
    14401 tagatgacga aaaaatggta tactttaaga gaaaggtttt caaaagaatc cttgtagaaa
    14461 aaaatgctga agatttaatt gttttaattt gggcgacagg tgagctaaaa gatgataggt
    14521 ttttgaaatt gcttcatcag ctcacaaacc acagacactc agatattaga agaataactt
    14581 attcagcaat cagaaagatt gaatcaccaa gtagtagaga agtactgcaa aaaggtctct
    14641 acgacaaaaa tgcacagact aggcagtact gtgcaaaagc tctttcaaaa ttgggagatg
    14701 aaaacagttt aaagatttta cagcagttga aagacaagaa taagaatttt gaaaaagaat
    14761 atgtgcttag agcttatgac gaagccatta agtcattgga aaatgcaaaa aatttgaaaa
    14821 agtaattttt tattaaagca atttgtttac ttaatgatgt atttctaaag tttaacaagg
    14881 taataaataa attaatggga ggggtttcaa atgatagtaa gacgttgtgc aggtggagta
    14941 gtgttttatg ctaataaagt acttattgta aaaaatgata ggggtgaatg gacacttcca
    15001 aaaggaaaga tactaggagg gggacttcca tatgagagtg ctgttcaaag agttaaagta
    15061 gaaactggca tagatgctaa aatgatagat gtggctggcg atactatgta tgaatttttc
    15121 tcaagaagta gacaacaaga agtttgtaat gcaataatgt ggtatgttat ggaagcttgt
    15181 aacactgatt gtgtacttgc tcctgaattt caagaaggtg gattttataa agtaaaggat
    15241 gctttagaaa tgttatcaca tcataaagag caagctttag tagaagtatc ttataaaaag
    15301 tttaaggaat taaagaaatt agtttcagag ggcgatgatg ttccagcaaa ttaattgtga
    15361 atttagagga ttctgaggaa gggatagccc cttccttttt taattgaagc agttttaagc
    15421 gtaaattata tagtctcttt aaactatgat ttatcaattt agagatttag tagtttatat
    15481 ttaaaatcag taagatatat ttaaaataaa aattaaaaag tatacaattt tattatataa
    15541 ttgtcttatg atgttgatta aagaagtatt aaaagtccat ttatttttaa atagaatgat
    15601 tttataaata tatatttgat gtgtatatta gaaaatgatt aaaataggag gaaatacaat
    15661 gagcaattac aggacattac atgaatttgg aacggatgaa ataacgattg aaaagtctgt
    15721 ttttattgga tatgctaagc ctataaagag tgaagaagaa gcattagagt ttataaatga
    15781 aataaagaaa aagcataaag atgcaactca taatgtgtgg gcttatactg taggtaaaaa
    15841 tatgaatata caaagatata gtgatgatgg agaaccacaa ggaacagctg ggatacctac
    15901 attagaggtt ataaaaaaag aagatttgag agatgtagcg gttgttgtta ctaggtattt
    15961 tggaggaaca aaattaggtg caggtggtct agttagagct tatacaaaag gtgctaaact
    16021 gggacttgaa gcaggtaaga taatttataa ggtaatgtat caagaagtta aggttaagat
    16081 agattatact caacttggaa aagtgcaaaa tgagttaatg aatttggggt attttataaa
    16141 agatactgta tatgaggata atgttgaaat agtagtatat tcaagattag aagatgtaga
    16201 gaaattatca gaaaaaatga ttgatataac aagtggaaca ggaaaaatag ttttaggaga
    16261 ggaattttat ttgtctgaac aaaatggaga aattttaata taatctaaaa ggttattaga
    16321 atagttaata cctcaaatag ccaattagta tttttaagtg aaaagccatt aaattgattt
    16381 aatctaaatc aatttaatgg ctttttatcg gattttgcac agaatccttt ttttacttca
    16441 aataaaccga ctaaaagtaa aaatatccca aatatttttt ttaatatatc tgaagacaga
    16501 taattagcta atagtgaacc aaatacagct cctaaaaccc caaacacagc tataggtata
    16561 accaatttaa aaattacatt tttatttttt atatgtatag caagtgctat aagtgtcata
    16621 gggatagatg aaagtaaatt tacgctttgt gctattttag tgtctatgcc agcaagtaaa
    16681 acgagtgcag gtataaggat tgttcctcca cccattccca ttcctccaat aattcctgca
    16741 aaaaatccaa ttagagcaaa taacattaaa acaccatcct taatgctgca attatcataa
    16801 ttattccaaa agaaattcta agaaactttc cagtaacctt atttagtaat ttagctccta
    16861 taataccacc aagaatactt ccaactgcaa ctttaattgt taagttaaca tcataagtac
    16921 ctttagatac atataaaaca gaacttgctg tcgttaagaa tattattata gaaagagcag
    16981 ttgcatgaga tttatgttct tctaccttaa cgatgtcatt taatattgga actaaaagag
    17041 tacctcctcc agaaccaaaa actccattta taaatcctgt aaaaactcca attatcgtat
    17101 tttttaaatt aaaatcaaag tattttttct ttaaatcatc agtttttttc ataaaaatca
    17161 ccttctgaaa tagttttacc aaaataggca aattatattt atataaaata agattaaata
    17221 taatctttca tgtttgttta attttgcata aaaaaaaaca tattaaaaca acatattaat
    17281 atatggtata atatattaaa acaaagtagc aaatttgaga ggagataaca atgtcaaata
    17341 taaaaataca aatagacaaa actgtagaat ggcttataaa taaggttaat gaggcaaatg
    17401 caaaaggatt aattgttggt gtatctggag gaattgattc ggcagtagtt gctaatttaa
    17461 ttaaaaaggc atttccagaa aactctatgg gagttataat gagcataaaa agtaatcctc
    17521 aagatagaga agatgcactt aaggttatag agggatgtga tatagaatac cttgatttag
    17581 accttataga accacaaagt gctatattag acatggtagt aggaaattta aaagataaac
    17641 atttatatag agaagaatat ttaaagatga cagatgcaaa cttaagagca agagtaagaa
    17701 tgagtacaat ttatacaatt gctaacaatc taggatatct ggttgttggt acagacaatg
    17761 ctgcagaaat acacacagga tattttacta aatttggtga tggtggagtt gatattttac
    17821 ctattgcaaa tttaactaaa ggagaggtgt atgaatgggc aaaagagctt ggtgttcatg
    17881 aagatttaat aaacaaagct ccttcagcag gtctttggga aggtcaaaca gatgaagatg
    17941 aaatgggaac tacttacaat atgatagatg ctgtattaga aggtagattg gatgaagttc
    18001 ctaaaagaga ccaagagata attgaaagac ttcatagatt aagtgaacac aagagaaaaa
    18061 cgcctgcaca acctcctaag ttctaattta ctatttataa gatttgtgat aaaatttaca
    18121 agtgatgaaa attagaataa ttaattgtac gtattaagga gtggaaatat gggacgtata
    18181 ggtaatataa ttaatagaaa aggtaaacaa gatgctcaaa gagcaaagat atttactaaa
    18241 catgctagag caatagcagt tgcagctaag gaaggtggag cagacccaga gtacaatgca
    18301 gcgttaaaaa cagctataga aaaagctaag gcagataata tgccaaatga taatatagat
    18361 agagctatcg ctaaaggtgc tggagcaggt gctggagaag actatgaaac tatagtttat
    18421 gaaggatatg gacctggtgg agttgctgtt atagtagaaa ctttaactga caacaaaaac
    18481 agaactgctg gtaacgtaag atactatttt gacaaaaacg gaggaaactt aggaactagt
    18541 ggatgtgtgt ctttcatgtt tgataaaaaa ggacaaatat tagttggttt aggagatgga
    18601 gtttcagaag aagaattgat ggatgttgca ttagaagcag gagctgaaga tttcataaca
    18661 gaagaagatg gttatgaaat aataactaca ccagaagatt tttctagtgt tcgtgatgag
    18721 ttaaaagcta aaggatatga gtttatatct gctgatgtga agatgatacc tcaaactaca
    18781 actgtattga ctgaagaatc tcatttaaaa atgatgaata aattagttga tatgcttgaa
    18841 gaagatgatg atgttcaaga tatatatcat aactgggaag tagaataaat agtataagca
    18901 tattgaaatt tataagtatt gaatcaattt taacatgtga taaattttat agaataagaa
    18961 acactaaaat agacatacgc acctctaacc tatatttgca taataatatg taagcatagg
    19021 taaggggtgt ttttatattt ataggttaag aaatttaaag taaattttga gctggactcg
    19081 attgtctata aataattgat gggtatataa cttgttgctg aataccattt atacgcccag
    19141 ttactgtatc aataatcatc ctaagactac gttctgctgt ctcttccatt tttaaatcta
    19201 taactgtcaa aggaggaata cttcttgcca ttagtcgtga aggtccatta cttgctgcaa
    19261 atatttgaat atcctctcca attacaattc ccgattcatg aaatgcattc atcataccaa
    19321 gagctaccac atcatgatta caaaaaatta ctttttttag tgaatttttt gcaagcattt
    19381 tttgagcaat atcatatcca ctatctattt tattagaaca ataatatata gaatctgata
    19441 gatgtattcc atgttcttta catgttttta aaaaagattt accccttaaa gtcatacatg
    19501 taaaggaagt tgggttgaat acaacagcaa tatcattttt accattttga atagcatgta
    19561 aagctgctat acgaccagtt tcctcattat caatagatac acttgaaaaa ccaggtattt
    19621 cacgatttac aacaactaca ggaattttag gtttattttt tactagatat tctatatcat
    19681 cagcattgat acctgttaaa acaattccat cataataatt atttgcaaaa aagtattcat
    19741 catcacatag atgaccaact ttgtaaggtc gtatgatgat atttatgttt aaatgttcta
    19801 aagaaattat gtggtttatt gcttgaagta tattggttga tgccaactca aatccctcat
    19861 cctgccaata aaaagctatt ttaatattat tatgtttaat aggagtagag cgtttatatc
    19921 caagcttatc agctgaattt aatataagct cttgtgtatg tagcggaatc ctgcgttcat
    19981 ttcctttacc acttaagacg tacgagacag ttgctgaaga aagaccacag tcttttgcaa
    20041 tatctttgat tgttgccatg ataaaatcct ccttaaatag ttgcttaaat ttgcttatac
    20101 caataatctt aacatatatt caactaaaga acaatattct acaaggtgca tattttacag
    20161 gattagaata aataaattaa aaaaaacttc ttacttatgt tgacataatt tagtttatag
    20221 gattataata tgcttaaata agcttatgat taacttgaat taagcaaagt taagcaggaa
    20281 catgcaatgc tagcttgcaa tgtgactgca aagggggaaa aggtatgagt aaggtaaata
    20341 tatttgaagt tggtcctaga gatggatttc aaaacctgaa agagttttta gaaactgata
    20401 aaaagcttaa gattattgat ggtcttattg cttctgggat taaacaactt gaaattagtt
    20461 catttgtaag cccaaaagca atacctcaga tgaaagatgc aaaagaagta gcaacttatt
    20521 gtgtagaaaa atatcctgat gtgaaacttt atgcattagt tccaaattta tatggtgcta
    20581 aagttgcatg ggagtgtggt ataagagata tatcctatgt tatatctttg agtgaaactc
    20641 ataataaaaa aaatataaat cgtacccatg aagagtctat tgatgagctt aaaaaaataa
    20701 tgaatactta tccagacatg aatatatgta ttggaatggc tactgtattt ggatgtcctt
    20761 ttgaaggttt accaaaaatt gaagatgcca taagatttgc taaacgcctt tgggaaaacg
    20821 gtatcaggga gataaatcta gcagatacaa tagggattgc tgaccctaag caagtaagag
    20881 aaactattag tgcattagtt gaagctttac cagaatgcag atggcaagta catattcatg
    20941 atactagaaa tatgggaatg gttaatactc ttgtggctat tgaatctgga attactactg
    21001 ttcagtctac acttggagga ttaggaggat gcccatttgc tcctggagca tctggcaata
    21061 ctgcaactga agatttagta tatatgcttg aacgtatggg gtatgataca ggagttgatt
    21121 ttgttaaact tcttgaatta tcaaaatatc agaagagttt tgtaaatgga gtatatagtg
    21181 gtcatcatgt aaatataaag tctgatagtt gtaattgtta gttctattga ttaatcgatt
    21241 aaaaaaatat gctgattggt aaaatagatt tattgataaa aaatataaat tataaaatac
    21301 attatctaat aaaacaaata aaaaaattga gggggattag tatggatatt atagctttac
    21361 ttttattttt aggagttatt attattgcat ttgtacgtaa gaataatgca ggtattttgg
    21421 ctctagctat tggtgtcata gctgttagaa tttttggaat ggatgataaa gaactttatg
    21481 gagctataag caactcaatg tttactacac ttgttggtat aacactactc tttgcaatag
    21541 tgaactcaac aggagcatta aagcttgttg cagaaaaaat tgtagctgct tctggtaaac
    21601 gtgtttggct tatacctata gcagttttta tagcaggatt tgttgttgct ggtgttggac
    21661 ctggggcaat tccagcactt gcaattatcc cagcaatggc tgttcctgta gccataacag
    21721 ttggttataa tcctgtaatg cttgcactta taggtgaatg tggattaatg gcaggtcgta
    21781 tgactcctat tactccagaa ggtcaaatta taacaaccgt tgcagaaggg gttggaattg
    21841 aaaatgttat gcctactatc ttagcctgtc aaactctttc tacaattgta ttttcaatag
    21901 tattgtttat aattttcaaa ggatataaac ttaaaaaacc tataaatgtt atagatagta
    21961 agcaaataga aaaattcaat ataaagcaga taatttctct aataagcata gtagttatga
    22021 ttgcttttat cataattttt gatatgaatg ttgggcttgc agcttttgca gtttcagctg
    22081 tacttatttt tttcaatatt gctgatgaag gagaatgtat caaatcaatt ccatggagca
    22141 cgatagtaat ggttcttgga gtaggtgcaa tgatgacaat cgtagatgca gcaggaggta
    22201 ttgacttgat gagtaatgca ttatcatcac tgatgaatag taaaacagct actccaatta
    22261 tgagtatttc agcaggactt atgtctatgg ttagttctgc tttagcagta gtttatccaa
    22321 ctatgatgcc tatgtgtgtt gacatagcta aggcagttgg aggagtagac ccactagcac
    22381 ttattgctgc agttggtgtt ggaggttctc ttgctggtgt aagcccactt tctactggtg
    22441 gggcattgat tttagcagct atgggttcaa gtaagaaaga ctttaataag gaagaacaaa
    22501 ataaggtttt tgtacagctt ttcattatga gtgcagttgg cttattggtt attgcagtag
    22561 tatcaatatt atcatttaat gcaataacac atttaatgaa ctaattagaa taactgaaaa
    22621 taaatcatta aatttaactt ttaatattaa tcaatataat ataatttgga ggggaagtta
    22681 tgaaaaaagg agcattaagc aatataaagg tacttgacct tacaagagtt cttgcaggtc
    22741 catattgtac aatgatgttg gcggattttg gagctgatgt tataaaaata gagatgccaa
    22801 gaaaaggtga tgatacaaga ggttttggac cttttgtaaa tggagagagt ttatactatg
    22861 caaatataaa tagaaataaa aaaggtatca ctctaaacct taaatcagaa gaaggaaaaa
    22921 atatattttt agaaatggta aaacatgctg atgttgtagt agaaaattac agaccaggtg
    22981 taatggaaaa gttaggtctg ggttatgatg agttggctaa agttaacaat caaataattt
    23041 atggagctgt atcaggattt ggatgttatg gaccatattc agaacgtcca ggttatgata
    23101 ttattgcaca ggcaatgggt ggtcttatga gtataacagg gcaagaagga ggagaaccaa
    23161 cgagagttgg taatgctatg ggtgatgtac taggtggaat gaatttaact attggtatac
    23221 tcacagcact caacgctaga aaaataatag gaaaaggaca aagagtagat gtttccctcg
    23281 ttgattctgt tgtatcgtct ctcgaaactg gtatacagag atactttgat tcaggacaac
    23341 ctgaacttat gggtaataga tatgcttcag cctatcctta tgactctttt aaggcaagtg
    23401 atggaagttt tgtaataggc tgtggtaatg acaaactttt tgaaaagtta tgtaaaaaag
    23461 ttctttttag agaagacctt ttaatagatg agagatttaa aacaaatctt aatcgttgtg
    23521 aacattacaa ggctcttaaa ccagaaatag aaaaatggtc tgttaagcat acaataaaag
    23581 aagcagttga cttaatatca gaggctggag ttcctgctgc tcctatctat aatcttgatt
    23641 gtgttacaac agatgaacat atagcaaaag caagagaaat gattgtagat attagtcatc
    23701 ctattatagg aaatatgaaa gtcaatggaa atccagttaa gttgatgaca acaaaagcta
    23761 aaattgatat gccagcacca tctctagggg aaagtaacga ggagatttat aatggttggc
    23821 ttggttttga tagtgataaa ataaaagatt taaaagaaaa tggagttatt tgattaaaaa
    23881 atagctaaac acacttgtga ctttagtcat gggaggttca gttagaataa tatcaaaaaa
    23941 tattaaatca atactgtaaa taaaatatag aattgtcaga agcatttaag atgggaaaag
    24001 ctacttagac attgagtctt tgtggctttt cctattttca ttaagaattt tatattatca
    24061 gaatagatat gtactaaact aaagtattaa caatttaaat taatagaaaa taaactacct
    24121 aattatgttg aataaagact actttagtga aaataaactt atagagatgc tatttaaatt
    24181 attttaatgg aaatatcaaa aatgagactg cattctcaaa aatgagactg cgttttttaa
    24241 gtattttctc gaaaatgaga caaaaatatt gttttatggg ttttattgaa gaaaaattct
    24301 tctgtgaatt acaatttaat caattttaaa gttgaaaatt attaaaaatt tagtgaaatt
    24361 tatgtatatt tcgtttattt tattaaaaat aacaattttt tactaaaaat aaagaacttc
    24421 aatggtgatt ggcatagtac ttgctatttt atattaacaa gaaatgtaga aaaaataaca
    24481 atgtataatg gggggaatat gatggaatct gtaatggaaa atttgaacaa tcttaaggtg
    24541 gaggttaagg acaaagtctg tgttattact ataaatagac ctaaagcttt aaatgctctt
    24601 aatagtgaca ctcttaggga actatcacaa gttattgatg tagtatcaga aaatgaggca
    24661 atattaggag ttattattac tggagaaggt aaagtttttg tagccggtgc tgatatacgc
    24721 cagatgcaaa actataaaag tgaggaaggc agaaaatatg caggttatgc tcagggaatt
    24781 tttgataaaa ttgaagctct tgagaaaaca gtaatagcag cagttaatgg atatgcatta
    24841 ggtggtggat gtgaacttgc tatgagttgt gatattagaa ttgcatcaga aaaggctata
    24901 tttggacaac ctgaagttaa tttaggagtt attccatgtt ttggtggaac tcaaagatta
    24961 tcaagacttg ttggaactgg aatagctaaa gagttaattt ttacggggag acaagtaaat
    25021 gcagaagaag ctaaatctat tggacttatt aataaagtgg ttccaagtga tttactttta
    25081 gaagagtcta tgaagatgat gaaccaaata gttgaaaaag cgccaatagc aataagatat
    25141 gcaaaagttg ttattaacaa agggatagat atggatttaa agaatgctct tgaactagag
    25201 aaagatgttg caggtttaac ttttgctaca agagataagc aagaaggaat gaatgcattt
    25261 atagaaaaga gaaagccagt ttttgaaaat aaatagctat tataactaaa attggaacaa
    25321 tgtagaatat attcaaaatc taaatttcgt gaaaaaatac atatagttta attttaaaag
    25381 gagttaattt atgaatacgg gagtaattat aagtttagta gggattatca tagctctatc
    25441 tcttctagtt gtaatggtta tgaagggaat aaatattttt attatagcaa ttgtatgttc
    25501 atcattagtt gctataacag gaagcctaaa tgtgtatgat gcacttaaag tggattatat
    25561 gacagggttt gttggatttc ttcaggcaaa tttctttatt ttcctaacag ggacattaat
    25621 gggaaaaata atggagatta ctggtggagc aaaatctatt gctaagatga ttgtaagatg
    25681 gattgggaag gataaagcac tactttctat tccaatagct tgtggaatat tagcctatgg
    25741 tggagtaagt gttttcgttg taagttttgc agtgtttccg atagctttag aaatttttaa
    25801 agaagctgat ttgccacgaa gatttatacc agcagctttg acttttggat gctcaacttt
    25861 tgctatggtt gcaccagggg caccacaaat tcagaatatt gttccagcat caacattagg
    25921 gacagatatt atggctggag cagtaaatgg ttttatatca tgtgcagtaa tgtttatagt
    25981 tggaagtaca attttgtaca aaatggtttc aaaagaaaaa gccaatggtg gtcattttat
    26041 agcacatgaa tcagatatat ttgatgaagc agaagcaact aattctaaga gagaaaatgg
    26101 tcccaatggt gtaattgcct taattccatt gatagtgtcg attttaataa ttaatgtaaa
    26161 agttaatgga aaagcgatag taccaattga ggtgggagta tttattggag ctttattagt
    26221 atatttattg ttaaataaat atcaagataa tactaaaata gttggacatg ttggtgatgc
    26281 atgtaaaact acagtagtag caatatgtaa tacttgtgct gtagttgcat ttggttcagt
    26341 agtaaaatca gcagttggtt ttgacttcat agttaatgct atgactaata ttcctggtcc
    26401 tcctattgca ggggctgcag ttggaactac agttattgca ggtatttgtg ggtcagcttc
    26461 aggtggacta ggaattgcag ctccacttct tggaccagta tttttagcca agggtgtttc
    26521 cgctgcagca ttaactagga ctatggcaat ttcatcagca gcattagatt cacttccaca
    26581 caatggatat attgttactg taacaaatgg tctttgtaaa gaaacacata aggatgccta
    26641 tatgccaata ttctgggtaa ctgttttaac cccatttatt ggaaccatag tagcagtcct
    26701 actgtttaca atgttcccgg gattgccata ggagaaaaga taaaaggtat aaattatatt
    26761 ttgatattat aaaatcaatt ttcaaaatat atacaataaa tttcatgttt taatactgta
    26821 aatatgtatt taaaataaca aattgaccat ataaggagga attgaaagtg ttaaataaac
    26881 cattagaggg agttaaggtt attgacctaa cgtattttgt agcaggtcca gggacttcta
    26941 aaatattagc tgactggggt gctgacgtta ttaaggtaga gccaagcttt ggagacccag
    27001 gaagaaagac aggggcaaca atgacaatgc caatagatga ttataataat cctttttaca
    27061 gcacatataa ttcaaataaa agaggtttgt ctataaatct taagagtgaa actggaattg
    27121 aaattatgga taagctttta agcgaagcaa acgtatttgt ttcaagttat agaacaggag
    27181 cattaaaaag actaggtctt gattatgaat cattaagtaa aaagcatcca caccttatct
    27241 ggggtcagat aaatggtttt ggcgattttg gacctgctaa ggataatgca ggatttgata
    27301 cagttgcctt ttgggcaaga agtggggcta tgcttgatat agcagaaaaa gatacaagtc
    27361 caataaatcc acctatagcc tttggagatg caacaacttc ttgtagttta tctggaggaa
    27421 tatgtgcagc tttgtaccag caagctaaga ctggaaaagg acaaaaagta atggcatctt
    27481 tatttgggca agcaatatgg aatgcatcat ctttattagc atctacacag tttagtgatg
    27541 agtatccaaa aactagaaaa aatgctattt caccagtaat aaattcttat cgttgtaagg
    27601 atggaaagtg gatattctta agtattttag agcatgaaag atattttgag gcactttgta
    27661 gattatttgg aagagaagac ttaataaaag aagagaaatt tgcatcttca gtagaaagta
    27721 agaaaaatgc tacagaactt attaatattt tagatggtga atttgcaaag tttactcaaa
    27781 atgagatggt tgaaaaactt actgctgctg atatagctca tgaaaaaatt caacatgtta
    27841 atgatgtagt gactgatgaa caggctattg ctaataacta tatatatgaa catacaagta
    27901 aagatggaaa taagacaatg cttgcatcta ctccagttaa atttggaaat attgaagtta
    27961 acatgacttg tgatgctcca cttataggag aacattcaga tgagatattg agagaacttg
    28021 gatacagtga ttctgatata gagactctaa ttgaatctgg aattgtaaca atgaataaag
    28081 aagcatgtga aatatgttaa taaacatgtg aagtatgtta ataaatagga gggaatttta
    28141 tggattttag attaacagaa gcacaattaa tgcttcaaag agtagcaaaa gaatttgcag
    28201 agaatgaaat agcaccaata gctgctgaaa cagataaaac aggtattttt ccaagggagc
    28261 tttttagcaa aatggctaaa ataggattta atggaattgg aacacctgta gagtatagtg
    28321 gttctggtgg ggcagatata gaaaaggtaa ttgttgttac tgagattgcc aaaaaatgtg
    28381 cagcatctgc agcaatatta tcaatacaca caatatatgc tcaagctatt ttgaaatttg
    28441 gtactgagga acaaaagaaa aaataccttc caatgatggc tgaaggtggg tgtgtaggtg
    28501 cttttgcctt aaccgaacct aatgctggtt cagatgctgc gagagctgca acaactgcaa
    28561 ttattgatga agaaactgat gaatatgttt taaatggaac taagtgtttt atttctggtg
    28621 gaggtcaagc agaatcatta ataatatttg cattgacaga cccatcaaag ggaataaagg
    28681 gaatgtctgc tatcattgtc gacaaaggta ctccaggttt ttcaatagga aaaattgaag
    28741 aaaaaatggg tattcatggt tcagaaactg cagagttaat atttgataac tgtagagttc
    28801 caaagtctaa cttattagga aaagaaggta aaggatttaa tatagctatg acttgtttag
    28861 atggagcaag aattggagtt ggagctcaag ctgtaggaat tgctgaaggg gctttagaag
    28921 aaagtatcaa atattctaaa gaaagagtcc agtttggcaa gccaatttca gctttgcaag
    28981 gtattcaatg gtatatagca gatatggcga caatggtgga atcagcaaaa ttattagtat
    29041 attatgctgc agatttgaaa gctagaggag aaaaacacac taaagaagct gctatggcaa
    29101 aatacaatgc gtcacgtact gcaagagagg ttacaaattt agcccttcaa atccatggtg
    29161 gatatggata tatgaaggat tatccattag agagaatgta cagagatgcc aaaataacag
    29221 aaatttacga aggtacttca gaaatacaca aagttgttat ttcaagagca gtactaggat
    29281 aggaggaaaa gaatgaatat agttgtttgc ttaaagcaag ttccagatac taatgaagtt
    29341 aaaataaata aagagacagg tactctaata agagatggtg taccaagtat aatcaatcca
    29401 gatgatagaa atgctttaga agaagctcta aaaatgaaag atgaactagg agcagtgatt
    29461 aaggttataa gcatgggacc tcctcaagct aaaagtgctt taaaagaagc tttggcaatg
    29521 ggagctgatg aagcttattt aatctcagat agagcttttg gtggttcaga tacttgggct
    29581 acatctacaa ttattgctgc tgctatagag aaggttggaa agtatgatgt aatattctgc
    29641 ggaagacaag ctatagatgg agatacagct caggttggac cagaggttgc agaattttta
    29701 gggataccac aagttactta tgcgaaagaa gtaaaggttc aagatgacaa attactagta
    29761 actcgttata cagagacagg ggattattta atagaagcaa agatgccagt tttattaact
    29821 gctataaaag agttaaacaa tccaagatat ccaagtgtca agggaatctt agaagcttat
    29881 aataatggag atgcaaagat tactgtatta acacttgcag atttagatgt tgatacaact
    29941 caaattggat taaagggttc acctacaaac gtttataagt cttttgtacc agttaaagac
    30001 aagcataatg aaataataga aggtataaat aaaaaagaaa aagctgagaa actaatagaa
    30061 atactattcg atttgaaact agtataggag tgttaagatg atgagagcaa aagttaatca
    30121 aggtataaat ttaaatgatt ataatggtgt ttgggtaata ggagagcaaa gggaaggtaa
    30181 aattaatcct gttacgatag aacttattgg tgaaggaaga aagcttgctg accaattagg
    30241 aaaagagctt gcagttgtga tagctggata tgaagtagag aaagaagtga aggaattact
    30301 tcactatagt gttgataaag tatattacat caatgaccct cttcttaaag atttcactac
    30361 tgatggatat gcaatttcaa ttgcaaattt aatcgaaaga aaaaagccag aggttgtatt
    30421 ggttggtgct acttctatag gaagagatat agcgcctaga attgcaggta aggttggaac
    30481 aggtctaact gctgactgta caaaacttga aatagattca acagataata agttattaca
    30541 aacaagacca gcatttggtg gaaatttaat ggccactatt gtttgtccaa aaaatagacc
    30601 acagatgtca acagttcgtc caggagttat ggcaaaagct gtaagaaacg aatcagagac
    30661 tggaatttta gaagttgtta ctccagaact tactgaaaaa atgattagaa ctagattagt
    30721 agagatactt ccacaagaaa agaaatctgt aaatctaact gatgctagaa taatagtatc
    30781 agggggaaga ggattaaaaa gagcagaagg atttgagctt ataaaagaat tggctgataa
    30841 attgggggca gagattggtg cttcaagagc agcagtagat tcagggtgga tagaacattc
    30901 tcatcaagtg ggacaaacag gaactactgt tagaccagaa ctatacattg catgtggaat
    30961 atctggagca atacaacatt tagcaggtat gagtgattct aaatatatag ttgctataaa
    31021 taaagatgca aaagccccta tttttagtat atgtgactat ggaatagtag gagatttata
    31081 tgaaattata cctgaaatga tagaatcatt aaataggtaa cttttagaaa acattgaata
    31141 tgtagaatga taagtcttat aatattgtat tgtaagactt attatttttg ctgaaggtta
    31201 tagtggggag atgaaacaat gaaagagcag tggtataagg atatatttgc aagagtatta
    31261 tctatgactg atgatggttt tattgttgta aatacaagtg gagtaataat agatattaat
    31321 gataaatatt gtgacttttt aggaaaagaa agaaaagata taataggaca aaatatacaa
    31381 agtattatac caaatacaaa aatgctagat gttatgaaaa ataagtattg tgaagaagga
    31441 gctatacatc attattcagg tggaaataca aaagaaaaaa gtgtaatagt aagtaggtct
    31501 tatgtggaaa atgacaatgg agaagtagtt gcaggagtag ctcaagttaa atttagattg
    31561 caatcatttg atgtagctaa aaagttaatg tcagaatata tggagctcca atattataaa
    31621 gagcagttta aggataactg tggttttgac aagctcatag gtgaaaatag agacttcata
    31681 gaacttaaga aaacaggtgt gaaagcttct aaaacaaact ttccagtact tttaacgggt
    31741 gaaacaggca caggtaaaga agtttttgct agagcaattc acaataatag cagtaggtca
    31801 gataaaccta tggttagtat aaattgcgct gcaataccag aagaattatt agagtcagag
    31861 ctatttggct atgacgaagg agcttttaca ggagcaaaaa agggtggtaa aaaaggtaaa
    31921 tttttagttg cgaataatgg tacaatcttt ttagacgaga taggtgacat gcctttgacc
    31981 atgcaagcaa aacttttaag agttcttcag gaaagtgaga tagaaccagt gggaggtctt
    32041 aaaactataa agatagatgt aagagtaatc tctgccacaa gaaagaattt aagtaaaatg
    32101 gttgaagagg gattatttag agaagaccta tactatagat taaatgtaat aaacattcat
    32161 atgatggaat tgaaggacag gcaagatgat attttattgc tagcaaatta tatattaaac
    32221 aaattaaatg ttgaatacaa ggaattgaag gttttaagtg ataaggttaa aaattgtttt
    32281 attaactata cttggccggg aaatataaga gaactacaaa atgtaataaa aagtgcctat
    32341 gctgtaagtg atgatatggt cattatgatg tgtgaccttc catctaagat ggataatatc
    32401 agcagagtag ctcaatgtaa tgtggatagc aattgttcaa ttcatgaaat ggttgagaat
    32461 tatgaaaaga gcttaattat tgatgtgtta aggaagtata attggaaatg ttctaaggca
    32521 gcagaggtta tgggaataca caagagtctt ttatataaga aaataaaaaa gtatgaaatt
    32581 gaattaaata attaagataa cttgaattaa ggcaatgcta aaaaagcgcc cttattcaaa
    32641 gcgaactgta taaactgttt tatgcagtta aaacgtgtaa aagtagatgt gaagtactaa
    32701 tgtcaatcat tagtactttt ttattatatt tataattact atctatattt cagtatcttt
    32761 tgatatctaa gattattatg tttaatttag tatataacaa aaccctctta gttatggaca
    32821 gttttttata ttttaactgt ctactaagag gggatagttt agtttaaatt tagagtaaat
    32881 tttatctagc atttataaaa aatattttga atagcttttt tgttatttag tcataatttt
    32941 ctacttcttt atcaagttct ttgccacaag aatcacactt atatatagca ttgtatccgt
    33001 aatcatattc atcaactatt ttcttttgtc taaatacacc accacactca cataggaaac
    33061 caatttttat aagtgtaacg ataaattgtg attttataaa ttttatatca ttgcatttat
    33121 cgttagtgta aaatgtataa atataagagt tatcgcactc atctcttaat tcaaatcttt
    33181 caatatatgt gttatttttt aattcctcaa attcttcttt agttaaatat gatttttgtt
    33241 gcaattcatg taaacgctta atttggtcca ttatttatat cctcccaata attttagtat
    33301 tttaagctta gataaggaat ctaagtttta ttaaatttat cccaaggaat tatttaaatc
    33361 atatttagtt ttattaatta caacatatat aaattacacg aaaataaaac taattgtaaa
    33421 aataatatcc tgtgaaaacc taaactacta accccttact aacccgaaaa ctaaaaaaag
    33481 aaaatatatc caaatataag tgatatataa ataaagatgt ttcatataaa tttaacttta
    33541 aatattct
//
LOCUS       DAYASV010000030        32447 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYASV010000030 DAYASV010000000
VERSION     DAYASV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.030"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(108..1064)
                     /locus_tag="ACGI68_001449"
     CDS             complement(108..1064)
                     /locus_tag="ACGI68_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFH0137893.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            1432..2220
                     /locus_tag="ACGI68_001450"
     CDS             1432..2220
                     /locus_tag="ACGI68_001450"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0137894.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(2343..2813)
                     /locus_tag="ACGI68_001451"
     CDS             complement(2343..2813)
                     /locus_tag="ACGI68_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HFH0137895.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            2952..3596
                     /gene="uppS"
                     /locus_tag="ACGI68_001452"
     CDS             2952..3596
                     /gene="uppS"
                     /locus_tag="ACGI68_001452"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HFH0137896.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            3720..4544
                     /locus_tag="ACGI68_001453"
     CDS             3720..4544
                     /locus_tag="ACGI68_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0137897.1"
                     /translation="MSKGNNNNNSRNKSKKTSHLNRKKRKLNKKKLAVLICFTVLFLF
                     IAFKATQGVVALVKSMDKSNKTNQQQNVNSEQFDLGNEEENKKKKYTVFIDPGHGGND
                     KGTESKTSNRYEKDLNLQIAKKLANKLSKQKDIQVVVSRTDDTYISLKDRAILANNSS
                     ADVLVSIHLNAEKNGNTATGIETWYRNKATDGSKELAQTVQSTIVSYVKVRDRGIVEN
                     NFEVLRESNMPAILIECGFLTTPSEEQKIINEKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            4859..6406
                     /locus_tag="ACGI68_001454"
     CDS             4859..6406
                     /locus_tag="ACGI68_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0137898.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            6409..6807
                     /locus_tag="ACGI68_001455"
     CDS             6409..6807
                     /locus_tag="ACGI68_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFH0137899.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            6847..8580
                     /locus_tag="ACGI68_001456"
     CDS             6847..8580
                     /locus_tag="ACGI68_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0137900.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            8812..10023
                     /locus_tag="ACGI68_001457"
     CDS             8812..10023
                     /locus_tag="ACGI68_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0137901.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIVQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            10216..10476
                     /locus_tag="ACGI68_001458"
     CDS             10216..10476
                     /locus_tag="ACGI68_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFH0137902.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            10830..12542
                     /gene="ptsP"
                     /locus_tag="ACGI68_001459"
     CDS             10830..12542
                     /gene="ptsP"
                     /locus_tag="ACGI68_001459"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFH0137903.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(13334..13999)
                     /locus_tag="ACGI68_001460"
     CDS             complement(13334..13999)
                     /locus_tag="ACGI68_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137904.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            14343..15773
                     /locus_tag="ACGI68_001461"
     CDS             14343..15773
                     /locus_tag="ACGI68_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0137905.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            16141..16719
                     /locus_tag="ACGI68_001462"
     CDS             16141..16719
                     /locus_tag="ACGI68_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFH0137906.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITISSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            16773..16895
                     /locus_tag="ACGI68_001463"
     CDS             16773..16895
                     /locus_tag="ACGI68_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFH0137907.1"
                     /translation="MKFASSLALSTAILSANSTCPWIIHQPKVPKEISNLKKTN"
     gene            17191..18570
                     /gene="scfB"
                     /locus_tag="ACGI68_001464"
     CDS             17191..18570
                     /gene="scfB"
                     /locus_tag="ACGI68_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HFH0137908.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            18599..19096
                     /locus_tag="ACGI68_001465"
     CDS             18599..19096
                     /locus_tag="ACGI68_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFH0137909.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            19394..21058
                     /locus_tag="ACGI68_001466"
     CDS             19394..21058
                     /locus_tag="ACGI68_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HFH0137910.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEAIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            21071..23521
                     /gene="recJ"
                     /locus_tag="ACGI68_001467"
     CDS             21071..23521
                     /gene="recJ"
                     /locus_tag="ACGI68_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFH0137911.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            23534..24046
                     /locus_tag="ACGI68_001468"
     CDS             23534..24046
                     /locus_tag="ACGI68_001468"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFH0137912.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            24120..26327
                     /locus_tag="ACGI68_001469"
     CDS             24120..26327
                     /locus_tag="ACGI68_001469"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFH0137913.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            26352..26801
                     /gene="dtd"
                     /locus_tag="ACGI68_001470"
     CDS             26352..26801
                     /gene="dtd"
                     /locus_tag="ACGI68_001470"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFH0137914.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            26816..27430
                     /locus_tag="ACGI68_001471"
     CDS             26816..27430
                     /locus_tag="ACGI68_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0137915.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            27521..29077
                     /locus_tag="ACGI68_001472"
     CDS             27521..29077
                     /locus_tag="ACGI68_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HFH0137916.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            29067..30329
                     /gene="hisS"
                     /locus_tag="ACGI68_001473"
     CDS             29067..30329
                     /gene="hisS"
                     /locus_tag="ACGI68_001473"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFH0137917.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            30403..32190
                     /gene="aspS"
                     /locus_tag="ACGI68_001474"
     CDS             30403..32190
                     /gene="aspS"
                     /locus_tag="ACGI68_001474"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFH0137918.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT        13143 a         3226 c         5684 g        10394 t
ORIGIN      
        1 taaaagtaat ttatatttta aaataaaaac acaccccata aatacaaagt aatttgcatt
       61 taagaggtat gtatttatca tcaaattatt ggtttgtttt ggaatttcta atcttcaacc
      121 ataatatctt taaatatgaa atcatgcatt gcatctagtt catattcctc aaaaggtatt
      181 acatagcctg cattacctat ttttctacca ttttcagtag aaagaggaaa ctccatagat
      241 ttcaaattta aatttcctat acttaaaagt tcttttccta aagatattat ttcattagga
      301 ttcatattag tttttacata cggaagtatt gtgttaacta gattaggata tttagtgact
      361 ggtaaatcct taacaccatt tattaaactc tgtataacac ttctttgtct tctatctctt
      421 tcatctgtac tatcattttt tcttatacga gaaaatgcaa gtgtctgata tccatttaat
      481 ttttgctcac cagaatttct tatatattta agagaacctt tattgtcatt tttattccat
      541 ttataagttt caggaataaa tttatttaat tctcttattt cactcttatc aacagtaaca
      601 gtaactccac ctaaagcatc aactatatcc ataaaagaga aaaagtcaac tacggcgtag
      661 ttttgtatat caagttggaa gttttcttct atagtttcta ttaataagtt agcttgtcca
      721 tatacgtagg catgtgttaa tttttgttct ccacgtcctg gtatattaac ataagtatct
      781 ctatttaatg aagttagctt aagactttta tttttattat caatagtaag tatcatcatg
      841 gcatcagaac gagaaacttc ttctcctggt cttgcatctg tacctaacaa taatatgttt
      901 gtgatatttt cttcgttttt atagtcatta ctactaagcg cctctgtatt tatactatca
      961 tcttgtatag tttttaattt agtaaaaaca tatccatata tactaacagg aaatgctata
     1021 actaaaaggg ctagaaatat aacgaacttt ttgagtcctg acaataatat cccctacttt
     1081 ctaaattttt taattacaca tagttaatta tattaaaaat ttgttacata agcaataaaa
     1141 aacacaataa attagcataa gtttgtcata atataattat ttttatagca ttttatagaa
     1201 gaaagtgtgt agttattatt gattttattg gaatataatt gtatgaagac ttaaattttt
     1261 ataaactgat gtgtatattg aaaaaaatat tttcctgaat gtagttatag catcttatga
     1321 aaggtgcaaa ttgtaattta aaatttttag tgtataataa gaattgaaaa ttcaaaccga
     1381 taaaaattaa taaaaaataa ttataaatta ataatttggg ggaggaaatg tatgataaaa
     1441 ttaatagcaa cagatttaga tggaactttg ttagatgaaa agagcgaaat aaatccagag
     1501 ttttataaag tatttaaaaa attaagagag agagggataa tgttttctgc tgctagtggc
     1561 aggcagtatc aaaatcttat aaaaaaattt gaagatataa aagatgatat gatgtttata
     1621 tcagaaaatg gaacactagt agtatataaa ggaaaagaaa tattatcaaa tcctttaaat
     1681 aaagaattag taaatgaaat catagagaca acaagaagta taaaagggaa aaaaattgta
     1741 atgagtggga aaaaatacgc ttatatagaa agcaaagatg aagcgtttat acaagaggtc
     1801 agtacttatt atgcaaagtt taaaattgta gaagatttga cgaaagtaga aggagacata
     1861 ttgaagatag ctgtatttga cttcaaaggt gcagaacata ataataatat ctactttgaa
     1921 aagttttctg atagagctca agtatgtatt tctggagtgg agtggcttga tttaactgca
     1981 aaaggtgcaa ataaagggag tgccataaaa aaagtacaaa aaatgttaga tataaaatat
     2041 gaagaaacta tggtatttgg tgaccagtta aatgatgtgg aaatgatgaa aagtgcctat
     2101 catagttatg ccatggaaaa tgcaaatgaa catttgaaac aaattgctag atttagagca
     2161 aaaagaaata ctgaaaatgg cgttgtagat aagataaaag aggttataaa aattggatag
     2221 tcatatgtat agatgaaaaa gattattttg aagaaatcaa aaaaatatca atgtaatttt
     2281 gataaataag tttatttatg cgaaaattac attgatattt aataaacatc aataatagat
     2341 atttattttt ttatgatatt cagtattttg ttttctattt gagttgcatt ttcagcagaa
     2401 attaaataaa catatctacc tgccttctca attatagtat tatttgctat ttcagaattg
     2461 attttttctc catgtccttc tccaaaaggt gcttgtatca tatctttttt ataattttct
     2521 agagttttat agattttatc tacctcgtca gtttttataa cgataatatc ttctagagat
     2581 aatttttcag aagaattaat tacaaaacct tgaattatgt cttcctctac atcattaaaa
     2641 tagttaaaat cctttgcatc aaagattttt gattccttta taagtaaatc agaagatttt
     2701 aatgcttttt ttatttttga tgtagaaacg tttttgtaat caccacttga agaacaacta
     2761 accattccta atgataagat tatcgaaaaa aacaatatta aaattttttt catgataaac
     2821 ctccaagttt tttaaactct gtcttaaatt agtttactac ataattatat aaaaataaat
     2881 gtatttaatg ttacagtttt gattaataat gtaatatttt gataataata cctattgaga
     2941 ggtgattaga aatgagaata ccaaaacatg taggaattat accggatgga aatagaaggt
     3001 gggcaagtca aaatggtatg acaaaagata aaggatatga aagtggaata gaccctggac
     3061 tagagtctta tagattatgt agagatttag gctgtgaaga ggtgactttt tatggattta
     3121 caactgataa tacaagacgt ccatcagtgc agacaaaagc ttttacaaaa gcatgtattg
     3181 atgctgcaaa tatattaata gatgaaggtg ctgctttatt agttgtagga aatacaaaat
     3241 ctccaatgtt tccagaagaa ttattaccat atactaatag aattaaagcc aaagaaggtc
     3301 aaataaaagc aaatcttctg gtaaattatg gttggtattg ggacttaaat aatatatcag
     3361 gagtagaaaa tgtaaataaa aataatatac aggattacat acagtcattt gatgtatcca
     3421 gactagactt gataatcaga tggggtggaa gaaggagatt aagtggtttc cttcctgtac
     3481 aatcaattta tgcagatttt tatgttatag ataattattg gccagatttt aagagtgacg
     3541 atataaagga cgcatttagt tggtatgata cacaagatgt cactttaggt ggataattaa
     3601 aaatattctg atttaaggtt gtagagtgtt ttaaattatt gaaaaaaaaa cttcttgatg
     3661 gtaaaataac actaataata tgtaaaagtg taaaaattta taccaagaag ggtgaagaaa
     3721 tgtcaaaagg caataataac aataattcaa gaaacaaatc taaaaagacc agtcatctga
     3781 atagaaaaaa gcgaaaatta aataagaaaa agttagcagt gttgatttgc tttacagttt
     3841 tatttttatt tattgcattt aaggctactc aaggtgtagt agctttggta aaaagtatgg
     3901 ataaaagtaa taaaactaat caacaacaaa atgttaactc agaacaattt gaccttggta
     3961 atgaagagga aaataaaaag aaaaaatata ctgtatttat agacccaggt catggaggca
     4021 atgataaagg aactgaaagc aagaccagta atagatatga aaaagaccta aatctacaaa
     4081 ttgcaaaaaa attagcaaat aaattatcta aacaaaagga tattcaagta gtagtatcca
     4141 gaactgatga tacatatata tcattaaagg atagagctat cttagcaaat aatagttctg
     4201 ctgatgtgct tgtttctatt catttaaatg cagagaaaaa tggtaataca gcaacaggta
     4261 tagaaacttg gtataggaat aaagctacag atggttcaaa agaacttgca cagacagtac
     4321 aaagtactat agtatcctat gttaaagtta gagatagggg aatagttgaa aataactttg
     4381 aagtacttag agaatcaaat atgcctgcta tattaataga gtgtggtttt ttaacaacac
     4441 cttcagagga acaaaaaata ataaatgaaa aatatcaaga ccaacttgcg gaaggaattg
     4501 ttcaaggtgt actttcatac ctagattcta aagggaataa gtaagttaac tttactttaa
     4561 tgtgaggata aatttaaaag tttaactgtg taaattgtat taactttatt tcgtaatgtt
     4621 gcaaacattg tttgaattta gaaactatga aattagaaat tattttatct atatctaatt
     4681 tcatagtttt ttttgcaaaa aatttatagt tataaaattt taaaaaataa atttagtata
     4741 atgtaatgta gttatttata tgtgatttta acttgcattg atttgctatc aattgtgctt
     4801 tataattagt atatataaag tttaactgta agataattta aaaaataggg gaatttatat
     4861 gaataaaaat agcatgacaa gaatttacag tttaaataaa ataattttta ttataattgg
     4921 aattttagga atagttttac tcacaagaca tattgaggta gatattaaag ttttaatttt
     4981 aattttatta accttgttta gtataaatgt atttatttca tatcttaaaa ttaagttgta
     5041 tgaggataaa attaatttag gattgataaa taattcagat aagtttataa atattactaa
     5101 agatgagaac ttcctaaaac atgttcaaaa ttatattatc agtaatgaaa aagaaaactg
     5161 tgtaatggct tgttttgatt tatgtagact caaacttata aatgatatat atggttatga
     5221 gctaggggat gaagtgttaa ataatatttt aagtaactta aaaaattatt ttggaaaaga
     5281 agctatttat ggaaagttaa aaagtgatgt ttttgtatta ataataaagc ttgaaaatag
     5341 ggaacagaag attccttatt tagtaaatct tattaagaat aaaattgtga tactaagtca
     5401 gattgacagt tttaaaatgg atataaatat tgaggcttct attggaattt ataaatttaa
     5461 caataatgaa attgatataa aaaaagctat agataatgct gatatggcaa gacttaaatc
     5521 aaaaggacta aagcacatag agtatgtaga atttgataat gctatggaag aagaaataca
     5581 atcaattatg aaaatagaga gagatttatt cttggctatt aaaaataaag agtttgtaat
     5641 tcactatcaa cctaaagttg attcaagtac aggtaacatt ataggttcag aggcattgat
     5701 aagatggttg cacccatcat taggcatggt tggaccaaat aagtttatac ctatagctga
     5761 aaaaaatggt ctgataaaca atattggtag atggctaatt caagaagttt ttattaccat
     5821 taacaagtgg atttcagaag gtataaatgt cttaccagtc tcaataaatc tttctagagt
     5881 agaactatat aaaaatgatt tagttgattt ttttaaactg atgtttaata catataatat
     5941 tccaaaagaa cttatagaat tagaaattac agaaacgaca gcactaagag atgtaaaatt
     6001 tataagtgaa aggctttatg agattaaatc tttaggtatg aatatatcat tagatgattt
     6061 tggagtaggt aattcaaatt ttatcaacct taaggggata ccactagata ttataaaaat
     6121 tgataggtca cttatattgg atatagttac aaatacaaaa acaaagttta tggtgaaagc
     6181 aattgttaac ctttcacatg atttaaacgt aactgttata tgtgaagggg tagaggatat
     6241 gcatcaggtc aaagtcttaa gtgaactggg atgtaatttc atacaaggtt atgtattttt
     6301 taaacctttg gatgagatga attataaaaa acttcttact gacggaagtt atataaatct
     6361 gaaagatact cttttagata aatgcatgac agttgaggag gagtaaggat gatttcagaa
     6421 gatgtagatt attcaataag aatcataaga gctatattta ataatggaga agataaacca
     6481 atagatgcta aaatgatatt aaaaagtgaa gatataccaa tactaacact atacagactg
     6541 cttagaaagc tttgtgcaaa caatattctt acttttaaca aagaagatgg caagacatac
     6601 atgttaaatg tgtcattgga tacaataagt ctaagaacta ttgtagaagt aattgatgga
     6661 aaaatatata taaataaatg tacgagatat gtatctaatt gcagtaattg cttaaaaggt
     6721 tgtaaagtaa atcgtgaatt gctagaaata gaaaaagaaa tttataaact tttatcgaga
     6781 aaatctataa gacaaatact agaataaaaa attttcagct atgataataa ttggaggttt
     6841 tcggttatgg atgatatatc tcaagataat tttctactgt ctaaagagta tgagaatagc
     6901 ttagatgtag atactaaaaa agcaagtgga atatattata ctcctaaaat aattgtagat
     6961 tatattgtaa aaaaaacttt aaaaaatcac gatataatta aaaatccata tccacgaata
     7021 cttgatattt cctgtgggtg tggaaatttt cttttagaag tatatgatat tttatatgac
     7081 ttatttgagg aaaatatata tgaattgaaa aagaagtatg atgaaaatta ttggactgtt
     7141 gataacatac accgtcatat attaaactat tgtatatatg gtgctgatat tgatgaaaaa
     7201 gctataagta tattaaaaga tagcttaaca aataaaaagg tagtaaatga tttagatgaa
     7261 tctgatataa agattaatct attttgttgt gatagtctta aaaagaaatg gcgatataag
     7321 tttgattaca tagtgggtaa tccaccctat attggtcata aaaaacttga aaagaaatat
     7381 aaaaaatttc tcctagaaaa atatagtgaa gtttataagg ataaagctga cttatatttt
     7441 tgtttttata aaaagattat agatatttta aagcaaggag ggattggcag tgttataact
     7501 ccaagatatt ttttagaaag tctatcaggt aaagatttaa gagaatatat taaatcaaat
     7561 gtcaatgttc aagaaatagt ggatttttta ggtgcaaata tatttaaaaa tataggggta
     7621 tctagttgta ttctaacttt tgataagaaa aaaacaaaag agacatatat agatgtattt
     7681 aaaatcaaaa atgaggatat atgtataaat aaatttgaaa cattggaaga acttctaaag
     7741 agtagcaaat ttgaacattt taatataaat cagagattgc tatctgacga atggatactt
     7801 gtaaataaag atgatgaaac tttttataat aagatacaag aaaaatgtaa atatagcttg
     7861 gaagatatag caataagttt tcaaggaata attacagggt gtgataaagc gtttatttta
     7921 agtaaagatg atgtaaagtt gaaccttgta gatgataaat ttttaaaatg ttggataaaa
     7981 agtaagaata taaataaata tatagtagat aaaagtgaat ataggctaat atactcaaat
     8041 gatatagata atgagaatac aaataaaagg atactagatg aaattatagg gctatataaa
     8101 acaaaattag aaaacagaag agaatgtaaa agtggaataa ggaaatggta tgaacttcaa
     8161 tggggtagag aaaagctttt ctttgaaaga aaaaagataa tgtatcctta taaatcaaat
     8221 gaaaatagat ttgctataga ttatgataat aatttttcaa gtgcagatgt atattctttt
     8281 tttataaaag aagagtattt agataaattt tcatatgaat atctagttgg aattttaaat
     8341 tccagtgttt atgataaata ctttaaaata actgctaaaa aaatgagtaa aaatatatat
     8401 gattactatc ctaataaggt tatgaagata aggattttta gagataataa ttatgaagag
     8461 atagagaatt tatcaaagca aataatatca attttattaa ataagtctat agataaagga
     8521 aaagtagaaa aattgcagat aaaaatggat aacttgataa tggattcttt aggtatataa
     8581 gcagttgtaa gtattaaaaa attataatct tttaattgtt tatttaaatt tagaaataaa
     8641 aagttagcca tataaaaaat attaaaaata tatacttttc aatatttttt tgataaaata
     8701 agaatgaatt tgtgcaattt tatttttctc caataactaa aaatagtttt ttgaattaat
     8761 atttgcttaa gaataaaaat cagaaacgtt tttgatatag taggtgataa tatgaaagga
     8821 ttagttttag aaggtggagg aacaaaagga gcctaccaaa taggtgcata taaagcttta
     8881 agagagcttg gtatagagtt tcaaggagta actggcacat caattggtgc tttaaatgga
     8941 gcatatatag ttcaaaatga tattgaagta atggaagata tttggttaaa ttatgactat
     9001 actcatttta tgaatattga tagagaaacc tatgagaggt ttaaaaatgt agatttcact
     9061 actaaaaatc tcaatgcagt gtttgagctt ataaataaag ctagaaaaaa tgaaggtata
     9121 gatataagcc cattaagaca actaatggag acaacattaa atgaagacat aataaggaaa
     9181 tcaaaaaaag actttggact tgttacggtc tggtgggata aaaaaattaa tccatatcca
     9241 ttgtatatcg aagatatacc taaaggaaga ttgattgatt atttaattgc tagttccagt
     9301 ttgcctattt ttcaaacgaa catgatggat gataagttgt atcttgatgg aatgttttgt
     9361 gataacatgc caataggact cttggaagac aaaggttatg aagatgttgt tgtaattagg
     9421 ttgattgatg attttattgg gaaaatgaat ctaaatcgac atcaaaatat aaatataaaa
     9481 actataatac catcagaata tctaggaggt tctcttaata aagatagaga taatgtagaa
     9541 aaaaacatcc gactaggtta cttagatact atgaaagcat atgaaagatg cactggaatt
     9601 aagtattatt ttgatgtaga gtataaatat aatgaagaat attgttttga taaatttaga
     9661 agtatgagca atgaaacaat acaatatatc tgtaatttat tgaatataaa aagagatgct
     9721 tcacttagaa ccgttatgga aagtgtaata ccaaagcttg gtgaggtgct tggattgcca
     9781 aaggattttt catataaaga tttgttttac tgtatatatg aaaagaaact tgaagaaaat
     9841 aatataaaca ggataaaatt atatgatttt aacaaggtta tagatgttgt aaatacaaat
     9901 attgattcta atagaaattt agcttctaat tttgaagtta gacctaaaat tagtattaaa
     9961 aagaacaaaa acacaaaatt attgacaaat tgtattataa aagacataaa aaatcaaagt
    10021 taaataaaaa acaaaaaaaa atttgtaatt atactcgaaa aaagtgtata ttagtagtat
    10081 aatatttttg tagcactagt gggaacaaaa aatgaccata ggacaaaaaa gtaccactta
    10141 gtaccattaa agtaaaaaac aggaaaacaa ttttaaaaaa tataaaatgt aaagtttata
    10201 ggaggattta aaaaaatgga aaaagtagtt agtatcaaaa atgcaagtgg attacatgct
    10261 agaccagcag gaatgtttgt taagaaggca gctgaattta aatcaacagt agaggttgta
    10321 gcaaaagaca aaactgtaaa tgcaaaatca ataatgggaa taatgagttt aggtttagct
    10381 caaggagaag aattaaagtt agttgtaaat ggagaagatg aagaagtagc tttagctgca
    10441 atggttgaat tagttgaaag tggatttgga gaatagatat aataattttt taaaaatttg
    10501 atatttagaa tctaatatta cttaaagtaa agtaacagag aagtattaaa catatacaga
    10561 ccaacatttg tagatattta atttctttgt tactttcatg tttatttaat cagaagatta
    10621 agactattgt taattgggta taaaaatata aatattttag aaaatgaaat aagatatttt
    10681 taatcataaa ttcagagtaa aaatacagta acaaatagtt tataaaattt tataaaaaat
    10741 tataagtaaa taaaactata aaaatgtaaa ttatttatat aaatttataa agtttatgtg
    10801 ctagatgata ttttttagga ggtagggtta tggcttacaa aggaataggt gcttctcctg
    10861 gtgttgcttt agggaaagcg ttagtagttg aacatagtga attagtaatt gaaaaaaaat
    10921 ctatagataa tgttgaagct gaaattgcta aattagaaga tgcagttgca gtttctaaag
    10981 aagaattagt aaaagttaag gaaaaggctt ctgaagaatt aggagcagag gaagcagaaa
    11041 tatttgaagc acatttatta gttttagaag acccagagtt aattggttct gcaatagata
    11101 aaattaaaac tgaaagtgta aatgcagagt atgctttaaa tgaaataaaa gagatgtttg
    11161 ttagcatgtt tgagtctatg gacaatgagt acatgaaaga aagagcggca gatataaaag
    11221 atgttacaaa tagaatactt agacatatat taggaattaa agttgttgat ttatctgctt
    11281 taagcgaaga agttgtatta atagcacatg atttaactcc atcagacaca gcaactatga
    11341 ataaaaaaat ggttttagga tttttaactg atataggtgg aagaacttct catactgcta
    11401 tcatgtctag aactttagaa atagctgcaa tcgttggatt aaatgatata acttcaaaag
    11461 ttaaggatgg agattttgtg gttttcaatg gagacacagg tgaagtaatc gtgaatcctg
    11521 atgaagagac tataaataaa tatactgaat taaaagctaa gtatgaagat gagagaaaag
    11581 cattacagct attaaaagga aaaccatctg taactttaga cggaaaacat gtggaattag
    11641 ctggtaacat aggaacacct aacgatatag aaggtctaat aaagaatgat gctgaaggtg
    11701 ttggtcttta tagaacagaa tttttataca tggatagaga ttctttccca acagaagaaa
    11761 tacaatatga agcatataaa gctgtattag aaggaatgga tggaaagcct atagttataa
    11821 gaacattgga tataggtgga gataaagaat taagttattt aagtatggaa cctgaaatga
    11881 atccattctt aggttacaga gcaataagac tttgcctaga tagaaaagat atatttaaga
    11941 ctcaattgag agctttatat agagctagtg ttcatggaag attaagaatc atgttcccaa
    12001 tgatttcatc tttagaagag ttgttacaag ctaaagaagt tgtaaaagaa gttttagcag
    12061 aacttgattc tgaaggtgtg gcttatgcta aagatgtaga aattggtatg atgatagaag
    12121 ttccatctgc tgctgtgata tctgatgtat tggctaaaca cgtagatttc ttctctatag
    12181 gaactaatga tttaattcaa tacacttgtg cagttgatag aatgaatcaa aagataagct
    12241 atctttacaa tcaatttaat ccagctgttt taagacttat aaagactgtt atagacaatg
    12301 cacataaaga aggcaaatgg gcaggtatgt gtggagaatc tgctggagac caaaaaatga
    12361 taccaatact tttaggtatg ggattagacg agttctctat gtctccaata tcaatacttc
    12421 ctgctagaaa gttaataact tcagttaaag agtcagatat gaaaaaatta gctgatgatg
    12481 tacttaatat gggtactgct gaagaaataa aaagctatat agaaaagact tttaatatat
    12541 aattaatgtt atatagttac taaaattcat ttttctaaat aacaaaatta tattttacga
    12601 attaaaaagg agatgtttca aaataaaaat ttgaagcatc tcttttttat aaaagattac
    12661 taagaattac ttaatctttg taatagtcat tttgtgaaaa agcaaaactg cattgtggct
    12721 atagtatata tggatgttat tatcaataaa agtactatta aaatgaagat tcattctcaa
    12781 atgatatcaa aagtagttgt gatggagtaa gataatattt tgtgatttaa tagtaaaaat
    12841 taaaaaaata tggtattgaa aaataattaa aagtatttaa taatagaatt agggaagaat
    12901 aagtttataa gtaaaaataa ttgattacta gttaataaaa taaatgtgat atccaaataa
    12961 atcctaaatc taatttgagt tttaatatag atagtctaaa gtttgattat taaagattta
    13021 attgtaagtt gttacagttg atgatattga ttagagatta aagtttagag tagaaagtta
    13081 ccttaaattt aaaaatgcaa gataacccaa tctaaaaatt ataatcactc aataggttta
    13141 aggatttatt tgtttattat aaataggagg gtgtctcaaa atgaactttt tagtttatga
    13201 ggcactctct tttatataga attaaatata ttttattatt tcaacaatga aaaagaagct
    13261 tatctctatt ttgagacagc ctctttgtat actattatta atttaatcaa taacataatt
    13321 taaaactata ttattagtta agatttcctg ctttattagt aaataattct ttaatagcat
    13381 tgtttctgga aactaaatcg ttatcagagt tgtttgtttt tttcataatc actgaataac
    13441 gttttttatt tataaaaaat attaaaatct tatcatcatc aaaattgtat tctttatatc
    13501 tgtcaataga ctcataacct agatttagaa tgtttttgtc ttttatacta tctacatcta
    13561 aaatatatga tgggtcagcc tttagtaata tattaattaa acatttttct tcaccagcac
    13621 agaataactc catcttactt gatatagatt tagattgctc tgaagttatt tttgcaggaa
    13681 taaggttgtc attgtcatct cttagaagat aaccatgtgt gtcttgttga tatactaaat
    13741 taaccttttt cttaggaaca ctaatcattt tatctaaatg tacataatga tgattttcaa
    13801 caaaaccttc atttaatatt tcaacaaagt tattaatttt acctaggcaa aaagattcta
    13861 tagattcttt acagaattta tttgtaaatg gtattatgtt tacgttttcc tcgtctgtat
    13921 tccatgctat agcataatcc aacgaatata cttcttcctc tatatctgca aaattatatt
    13981 gtactatgtt tcctttcaaa attactcgac ctccatctta taatatattt aatatacttt
    14041 ataaaactta tattatattt aaataatatc ataaattgaa taaatatgat atagtaaata
    14101 agaaaaaatt tgaaaaaaat cactataaat gagtaataaa tataaatagt gtatactaat
    14161 gggatggaaa atgtccacta ttaactttat taccccacat atataagata aaattattaa
    14221 tataaaatat aatctatcga caatgaatag acttttaaat aaacttaata taaaattaat
    14281 taaataagtg cttaaaaacg atttacttta atgatagaat catattaaag gaggataatt
    14341 ttatggatat aaagaataaa attaaatcta tatttttacc agaaaaaaat gactatttag
    14401 atgatgaaga atttgagtat gatatagaaa ataatgaaga agatttaaag gaagataaca
    14461 aagtacattt aaatgattta agcagagtaa aatatgatta cagagagtta aaagacatag
    14521 aaaaccaaac agaagagatg tgtctgctag cagtaaagca ggatggtact ataattgagt
    14581 ttgtaaagga caagacttat aaagtatgta tggaagcagt aaaacagaca tataaatctc
    14641 ttaagtatat aacaaatcaa aatgaggata tttgtataga agcagtaaaa cagaactaca
    14701 gagctttgta ctacataaat aataagacag aaaatgtact aattgaggct ataaaaaatg
    14761 catcaactta tgatgtcatg gaagtattta aatttgtgga agagcaaaca gaagatgtat
    14821 gtctagcatt tatagaaagg gcatctaaaa atgatgttgc agagatatta aaaggaataa
    14881 aagagcaaac tccagccata tgtttagaag ctgtaaaaaa agatggaaaa tctttagctt
    14941 atgtaaagga acaaagtaat agtatatgtt tagaagctgt aaaagaaaac tacagtgcac
    15001 tatcttgtgt aaaagagcaa actgaagaaa tatgtataga agcagtaaaa cagaatgact
    15061 ttgcattgta ttatgtaaat gaacagacag aaaaaatatg catggaagca gtaaaaagaa
    15121 gttatatggc tctacagtat gtaaataagc aaacagaaga gatttgtttg gaagcagtaa
    15181 gaattgatgg aagagcatta cagtacgtaa aagagcaaac agaagaaatt tgcttggagt
    15241 cagttagaca aaatggaaaa gtactgcaat atgtaaaaaa acagactgaa aatatatgta
    15301 tagaagcagt aagaggttca tttgaagaac ttgaaataaa agaaatatta agttatgtaa
    15361 agataccaac agaaagaata tttgtagaag cagtaaagca aaatggaaaa attcttaaat
    15421 atgtagaaaa tcagacagaa ttaatatgtt tagaggcagt aagagaaaac tataatgctt
    15481 tagcttatgt aaaggaacaa acagaaaaga tttgtttaga agcagttaat caaagttatg
    15541 aagcgcttaa atatgtgaaa gaacaaacag aagaagtttg tctaaaagca gtaaaacaag
    15601 attatagaat gttgaaatat gtaaataatc aaacagaaaa aatatgttta gaagcagtaa
    15661 agcaaaatta tagagcttta gaatttgttg ataatcaaac agaaaaagtt tgtcttgaag
    15721 cagtaaaaca gaatagaaaa gcactacagt atgtaaaaca aaaacaaagt taaaaggcta
    15781 aaatatatgg ggaatatatg caaataaata aaatttgata ttttgtaata aataaaatgg
    15841 gtatctcaaa aggaactttt tagtttgtga ggtactcttt ttatatgaaa tgaaatatat
    15901 ttttagcctt ttaacaaagg ggaaaaggtt tgtttctatt ttgacatagg ctttttatgc
    15961 atataaagct tgataaaatt gttttattat aattgttttg ttatataaat aataagaaga
    16021 aaattatata ggtctttttt tgaccagttt tacattttta acaactagtt ttgtaaagta
    16081 tattgaattt tctcaaaaaa gaatgtactc tatatatgta aaattttttt ggaggtaaaa
    16141 atgtttaaaa gatatgctga aaaaatgaca tctgttttaa tttgtaataa tatgatagat
    16201 aataatgaat ctaaagtcta ttcttatgga tttgaaatat taatagcttt catagttaat
    16261 ataaccacca tgctgtttat aggtttttta tttggtaaat ttacatatgt attatttttt
    16321 ttaatgtgtt attgtccaat aagacaattt tcaggagggt atcatgcaga caattatttt
    16381 agatgcttat taacttttat ttttataatt ttatccacaa tcttaattat agaaaatata
    16441 aatatagact tatttaaaaa tattattatg attattgcaa gtgttagttg ggttggaatt
    16501 tgtgttctgt gcccaataga acataggagt aaccctataa gtgataggga aaagttggtt
    16561 tataagaaaa ctgctatatt tatatctaca gtagtgttat tgataacaat ttcaagtctt
    16621 tctttgagta tatttgtaga ttatttcaca tatagcgcat ttgccatgtt ttggattttt
    16681 gtgatgttag tattaggtaa gttaaaagct aaagtataag taaaatatgt atgagggaga
    16741 gttaagttat gaaaaagttt attgtaagat ttatgaagtt tgctagctca ttggcactta
    16801 gcactgctat tttgtcagct aatagtacct gtccttggat tattcatcag cctaaagtac
    16861 ccaaagaaat cagcaatcta aagaagacaa attaaataca agcacttatt tatacaaaat
    16921 tatcaggaaa aaagaaatta ttatatttta ataagattaa aacaatataa aatcagatac
    16981 aaaatttatt ttataggagc ctaataccta tttggtaata tattagtgcg ccaatttagg
    17041 gggatatagt gtatttaatg aaatgactta cggagagtaa gtcatttttg tattgtatgt
    17101 attttatgta aacttatgat ataatgaagg gtaaagttta attaaaataa taaaaaactt
    17161 tagtatagat agtaatttag gaggtagggt atgagtatga tacataaatt ttctatgaat
    17221 ggatataata tcgttttaga tgtgaatggt ggagctgttc atgtgttaga tgatgttgca
    17281 tacgatttgt tagattttta taaagaaaaa agtaaagaag aaatattaga gatattaaaa
    17341 tcaaaatatc aagaagaaaa aataaatgaa gcatatgaag aaatattaaa tttggaaaaa
    17401 gagggtcttt tatatacaga agatacatat caatatcatc caagctttgt acatagagag
    17461 cctgtagtaa aagctttatg tttaaatgta gctcatgatt gtaacttaaa atgtaaatac
    17521 tgttttgcag ctcaaggtga ttttggagga gaaaaagaac ttatgagttt tgaagttggt
    17581 aaggcagcaa ttgactattt gattgctaat tcaggaagta gaaagaacct agagatagat
    17641 ttctttggtg gagagccttt gatgaacttt gaagttgtaa aacaactggt tgactatgga
    17701 agaagtgtag aaaaagatta taataaaaat ataagattta caataactac aaatggtgta
    17761 ctgctaaatg atgagattat agattatata aatgaaaata tgcataatgt agttttaagt
    17821 ttagatggaa gaaaagaagt taatgataat atgagaccaa ctttaaatga taaaggaagt
    17881 tatgatatta cattaccacg atttaagaaa cttgtggaaa aaagagcaaa agataagtat
    17941 tattatataa gaggtacttt tacaagagat aatttagatt tttcaaaaga tgttatgcat
    18001 tttgcagatt taggatttaa attaacctct gtagagccag tcgttggaga tgagagtaat
    18061 ccatatgcac ttagagaaga agatttacct aagatatttg aagagtacga aaaatttgct
    18121 gtagagtatg cagatagaca attacaagga gatggattta aatttttcca ttttatgata
    18181 gacctaaatc aaggtccatg tgtaataaag agaataactg gatgtggagc tggaaatgag
    18241 tatctttctg taactccaaa tggagatatc tatccatgtc atcaatttgt tggaaatgaa
    18301 gaatttaaaa tggcaaatat atttgatgaa gaaatagtac ttccagaaaa tttaaaaaat
    18361 atgtttagag aagctcatgt atatactaaa gaagagtgta aacaatgttg gaataagttt
    18421 tactgttcag gtggatgtca tgcaaatgcg ataaacttta ataatgacat atcaaaacca
    18481 tatgaattag gatgtgaaat gcaaagaaaa cgtacagagt gctctataat gatacaagct
    18541 aagttaatgt tagaaggagc aaccaactaa atttatattt taaaggagtt gtttatatat
    18601 gattagagat tatttagaag ataaaccttt aattgacgaa agtgtatttg tggcaaaatc
    18661 agcagatgta attggaaatg ttaaaatagg caaagattca agtatatggt acaatgcagt
    18721 agttaggggt gatgaaggac caataactat tggagaaaac actaatatac aagattgttc
    18781 tatagtgcat ggagatacag aaactataat tggtaacaat gtaacagttg ggcataggtc
    18841 tatagttcat ggttgtaaaa ttagtgataa tgtgttaatt ggtatgggtt caataatatt
    18901 ggataatgct gaaataggag aatatactct gataggagca ggtacactaa ttacatctaa
    18961 taagaagttt ccgccaggtg ttttgataat gggttctcct ggtaaggttg ttagagagct
    19021 tacagaagaa gataaaaaat atattgatga atcttatgaa tggtatttag aggcagcaca
    19081 aaatcaaaaa tattagttta aagagtggtt gtttcaaaac atatttaaat ctgttttgag
    19141 gcaaccacta atttatttta ttagattttg ttgatatttt agttttcaaa aagtatttga
    19201 ttaaaccaga tataaaatgt caaaaataaa aattaactga gttaaataat tttaataaaa
    19261 ttggtagtat atgtgataaa attaaatatt aaagaggata taggatatta gaatatcaat
    19321 tggtgaaata atgtcatata cgataaatta aggtgtaagt ttttgggtat acctatctta
    19381 ggagtgaaaa gatttgagaa taagttatag aaatttaaag cgatatagag aaattggata
    19441 tgttttaata aagtatggtt ttagttttat tgtagaaaga ctaaatatag aaggaattgc
    19501 ctataaaata ccactatttg acccaccaga agaaataaaa aatatgacaa ctggtgagag
    19561 aatgaaaaga gttttagaag aactaggacc tacatatata aaaataggac agatattgag
    19621 tacaagaaaa gacttgttag accaggatat aattgatgaa atatcaaaac tcagagatga
    19681 tgtagagaaa tttgattcaa atatagctat tgatatattt aaagaagaag tgggtttaag
    19741 tatagaagaa atattcttag aatttaaaga agaagcaata gcagcagctt caattggtca
    19801 agtttatgaa ggtgtactta aaacgggaga agaagtaatc gtaaaaattc aaagaccaaa
    19861 tatagagaaa ataataaaat ctgatttaga gattcttaga actattgcaa atactttaaa
    19921 agatttaaaa aaggatttta atttagattt agtacagatg attgaagaat ttcaaactca
    19981 attaatgagg gaacttgatt atacatttga agctataaat gcaactaaat tctctaggat
    20041 atttaaaaat agtgatgaag tatatatacc taaagtatat tcagaatata atactaagaa
    20101 aattttagta atggagaaag ttaatggtac aaagttaagt gatgtagaaa aaataaggag
    20161 actaggttat aacacaaaaa ctattgttga aataggcgtt aggtcatttt ttacacaggt
    20221 attatctcat ggattttttc atgcagaccc acatccaggt aatatattcg tagttgctaa
    20281 aaataagata gcatatatag attttggaat gattggaatt attgataata aaactttaaa
    20341 ccaattaaat gaaattgcat tagcaggtgt agaaaagaat gttgacaaga taatatatct
    20401 attgatagaa atggatgcat taaatggaga ggctgatatt aagggactta gacaggattt
    20461 actttatcta atacattatt actatgatat ttctatagaa aaaataaatg taacagatat
    20521 attaaatgaa ctatttaggt tttttagaca gtataaaata gtcatgcctg cacaatttgt
    20581 aactcttgca aagacagtta taacattaga aggaacaagt agaactttaa acacagattt
    20641 ttcttttggt tctatgggaa aagagtttat gaaacatcat tataagagta aatttaaccc
    20701 taaaaatgtt gttttaagtt caagacaaaa tgtagaagaa atattactgg acataaaaac
    20761 tattccaaag caattaaaag caattttaaa aaatatagaa agaaataata taaaaatgca
    20821 aattgaagat gttaaaatga cgaggttaga aaattgtata atagagctaa catctcaaat
    20881 atctttgagt ttagtgttgg catctattat agtaggttct tctcttataa ttgcatctcc
    20941 aaatatagaa aataatatat ggataaaatt tactgcaata gcgggtttct ttatatcctt
    21001 tataataggc ctttgcttag ttataagaag tattaggtca aaatacaaaa aagattaggg
    21061 ggaattaaat ttgaaaaaat ggatattgaa gtataaaggt aagatagagg aaagtgaatt
    21121 tagcaaaaaa cttaatattt ccccggaaat ctgccaaatt ttaaaaaata gagggatatg
    21181 tactgaaaaa gattcagaga tatttatgaa tccatcttta gattatttaa gagacccttt
    21241 tttaatgaaa gatatgcaaa aagctgttga tagaataaag ttagcagttg agaagaacga
    21301 aaaaatttgg atttacggag attatgatgt tgatggtgta tcatctacat ctatcttgtg
    21361 cttatatttt aagagtatag gttacaatgt tgattactat attcctaata gattagaaga
    21421 agggtatggt ataaatgaag atgctattaa gcttataagc tctaggggat gtgacttgat
    21481 tataagtgtt gactgtggaa ttacatcagt gtctgaggtc aatacagcga atgatttggg
    21541 catagatgtt ataattacag accatcatga atgtcaaagt gaaattccat cagcctttgc
    21601 agtaataaat ccaaaacaag aagattgtaa ttatccattt gattcactat gtggatgtgg
    21661 tgtagcattt aaaatgattc aagccttgac tccaaaagaa gaatttaaaa ctagtatgta
    21721 taattatttg gaaatagtaa cactagcaac tatttgtgat attgttccac ttatagatga
    21781 aaatagaata attgtaaaaa atggtttaaa atcaatgaaa gaaggcaaaa atattggatt
    21841 aagggagctt ataaaagttt gtggtgtaga aagtgataag ataggttctt ctcatatagg
    21901 ttttgcaata ggtcctagga taaatgcttc aggtcgattg ggatattctt acttaggagt
    21961 agagctattt actactcaat cacaagaaga ggcagtagaa atagctagta tattggaaga
    22021 aaaaaataat gaaagacaga tgatagaagc taagatgtat catgaagctg aggaaatgct
    22081 aaagtctaat agcagataca atgatgacaa ggtgttggtt ttagctaaag agggttggca
    22141 acatggaata ataggaattg tagcatcaaa gttaactgaa aaatactaca aaccaacaat
    22201 tcttctaggt atagaaaatg gggaggctac tggttcagcg aggtcaataa aaggatttaa
    22261 tatatttgaa gctctcataa agtgcaaaga cttgatgact aagtttggtg gtcatgaaca
    22321 agcagcaggg ttatccttgg attcagacaa tgtagaaatt ttagcaaatg aaataaataa
    22381 atttgcagat tataatttaa ctgaagatga tatgatagag aatgtaaatg ttgagtttga
    22441 attacaagaa aatgtaataa atttaaatct tgtagaagaa ttacataagt tagaaccctt
    22501 tggattaaat aatcctaatc ctagatttat agttagaaat tatattttaa aagatttgaa
    22561 agttataggt aagaatcaac aacatctgaa attaagcata gaaaaagaga aaagctatga
    22621 atgtatagga tttaatatga gtcatttaaa atcaatgtat aaagtaggag acaaggtaga
    22681 tgtactattc caattagatg aaaataacta tatgggaaat aggaaagtac aatttttact
    22741 taaagatata cgattggctc gacctaaaag tgcaagtaat gacaaacttt cattgaaatt
    22801 aatgtcaaaa ataataccaa aagatactca aagcctgtat aatatatctg tgtctgattt
    22861 tgaattattt gatggaaata cagatataaa tatatttgat tattttgaaa aagatacact
    22921 aataatatct aattctataa atggatttta tagagcaatg tcagatatat cacttataga
    22981 tttggatttt aacataaatt acaatattat tgaagatgtt agcaaaaata ctgataaatt
    23041 agagcttata ttttctccta atattgataa aatagactta aaaagatata ataatattat
    23101 tctttatgat tatttataca ataaaggcga atattcgtat atatatgaga ataaaagaga
    23161 agaaagtgaa attataaaat attataataa aacagaccta ttatatttaa agaatgtagt
    23221 tagtaatata gttccatcaa gagatgaatt tataacaatt tataaacaag cactcataaa
    23281 aaaagaaatt gatttggata tggtaaatat aagggaaact tttaatgtaa ttccattaaa
    23341 gttttttacg atattaaatg tatttagaga attaaattta cttgacttta atttaaatta
    23401 tgaaaaaaat tctgtactca taagaatatt gccaaaacca cagaaaaaat tagacttaaa
    23461 tgagagttta atactcaata atctaaaaaa tttagaaaaa caatacaata gcagttatta
    23521 ggaggcgttt cccatggatt taaaaaactt tataaggaat attgatgatt tcccaaagcc
    23581 aggcatagat tttaaagatg tgacaactct atttaaagat ggagatgcat ttaaatatgc
    23641 agtggattct atagttgaag aattgaaaga taaagatgta gatttagtaa taggaccaga
    23701 agcaagagga tttttgatgg gtacacctgt agcatatgct ttaggagtag gatttgttcc
    23761 aattagaaaa cctggaaagt taccaggtga agttgagagt tatgagtatg gattagagta
    23821 tggtacagat actttagaga tacataagga tgcaattaaa aagggtcaaa aagtagcaat
    23881 agtagatgat ttattggcta ctggtggaac tatggaagcc gctgccaagc ttgtagaaaa
    23941 attaggtgga gaagtagttt ctatgcaatt tttgattgag ctaaaattct taaatgggag
    24001 agaaaagttg tctaactatg atgttaattc tcttataaaa tactagtact tttataatag
    24061 ttggtataac accaactatt ttcatatata ttaaaatttt atacaaaata ggtggttata
    24121 tgcatgataa agaattacaa gaaataattg aaaaaataaa agtttatgca cctaatgcaa
    24181 attttgaaat ggttgaaaaa gcctataatc ttgcaaaaaa agcgcatgaa ggacaatata
    24241 gaaaatctgg ggaaccatac tttattcatc cattagcagt tgcaaatatt ttaatagaga
    24301 tggaattaga tattgaaact ataacagcag gtttacttca tgatgttgta gaagatacag
    24361 aatataccta tgaagacata aaaaaagaat ttggcaaaga agtagcggat ttggttgatg
    24421 gagtaactaa acttggtcaa ataaaatatc gttcgaaaga agaaactcaa tctgaaaatt
    24481 taagaaaaat gtttttggca atggcgaagg atataagggt tatacttata aaacttgctg
    24541 atagattgca caacatgaga acattaaaat acatgcctcc agaaaaagca aagtataaag
    24601 caaaagagac acttgaaatt tatggtggta tagctcatag attaggaatt tccaagataa
    24661 agtgggaact agaagacaga gcattaagat ttatggaccc agaagggtat tatgatttag
    24721 ttagtagagt atcaatgaag agaagtcaaa gagaggatta tattcaagga attgttgaac
    24781 ttcttaaaga taagtttaaa gatttaagta taccatgtga tgtatatggt agaccaaagc
    24841 atttttacag tatatataga aagatgcaaa aaaaacataa gacttttgaa gagatttttg
    24901 atttaacagc agtaaggata ttggttgata atgtaaagga ttgctatgca gtacttggta
    24961 tagttcatac attatggaga cctattccag gaagatttaa agattatata gcaatgccaa
    25021 aacccaatat gtatcaatca ttacacacca ctgttgtagg tcctgatgga gaacctttag
    25081 agatacaaat aagaactcat gagatgcata atatagctga aaatggtata gcagcccatt
    25141 ggaaatataa agaaggtatc tctagcaaag aatcaaaggt tgaagaaaaa cttcaatggt
    25201 tgagacagat gatggagtgg gaaaaagatt taaaagaccc acaagagttt atggatgcac
    25261 ttaaagaaga tgtatttaat agtcaagttt atgtatttac gccaaaagga gacgtaattg
    25321 aattaccagc agaatcaaca cctatagatt ttgcctatag ggttcataca aatgtaggca
    25381 ataaatgtgt aggagctaaa attgatggaa gaatagttcc tattgattat aagcttcaaa
    25441 atggtaatat tgtcgaggta cttacatcat ctaattctaa tggaccaagt agggattggc
    25501 ttaatatagt gaagacccca aatgcaaaaa gtagaattag acaatttttc aagaaggaaa
    25561 gaagagaaga aaatgttgac cgtggaaata caatattaga aagagaattt aagaagcatg
    25621 atataccaca aaaagaccct cttgttgaaa aatatatggc attagttgct aagaaattta
    25681 atcagccaag tgttgaggac ttaattgcca ctgtaggtta tggtggtata atgtcatctc
    25741 aagtggtatc taaggtaaaa gacctatatt taaaagaggt taaaaaagct aaaaaggaac
    25801 aacagataca agaagaagat ttaaataaac acaatataag tgaaacagaa tacagtaaaa
    25861 agagaaagaa aaacgcatca caaggaatta tagtaaaagg gcttgacaat atcttagtaa
    25921 gatttgctaa gtgttgtaat cctcttccag gagatgaaat tgtaggatat attactaagg
    25981 gtaggggagt agccatacac agagctgatt gcccaaatgc aaacaataat ggtgatttct
    26041 tccaaaatag attggtggag gtttcatgga gttcagcagg aaaaggtaag tttgaagcgg
    26101 aaattcaaat aaaagctatt gatagaagag gaattgttaa tgaaataact catattgttg
    26161 caatggataa acttagttta aacggaataa atgcaagaaa aggtaaggat agtattgtaa
    26221 gtataaactt acttgtagag gttaatgaca cagaggaatt aaaaattcta atgaaaaaat
    26281 taaaagcaat tcctggtgta gaaagtattt atagaatgac aaattagagt taaaataata
    26341 gggaggaaac tatgagagca gtagtgcaaa gagtatcttc atctaaagtt acagtagatg
    26401 aaaacactat aggtcaaata aataaggggt tactagtatt gttaggagtg actcatgatg
    26461 ataaatcaag tgatgtagat tatatgattg ataaaatatt gaatctgaga atttttgaag
    26521 atgaaaatga caagatgaat ttatctctaa tggatatagg tggagagctt ttagtagtat
    26581 ctcaatttac tttatatgga gattgcagaa aaggaagaag acctggattt agtaatgcag
    26641 ctaggccaga acttgcaaat aacctttatg aagaatttgt aaaaaaagca aaagataaag
    26701 gtgtgacagt tggtacaggt caatttgcag ctcatatgat ggtagagtta actaatgatg
    26761 gtccagtaac aatactatta gactcaagta aatcttttta agggggaaaa ctttaatgat
    26821 tataaaaaaa tttgtagata gttattttgg agtaaatact tatgtattag gtgatgaaaa
    26881 gactaaaaaa tgtgcagtta tagacccagg tggaagttta gtagagacat taagttttgt
    26941 aaaagaaaat gaattaaata ttgaatatat tatccttact catgggcatg gagaccatat
    27001 aggatatgtt aaggatatta aagaaaaaac tggtgctaaa gtagttgcac atgttgatga
    27061 aaaagaatta ttaaatgaca aaaataaaaa tctaagttat acaatgagat gtggagcgca
    27121 agagtttgat gcagatatct atgtaaatga taaagacaag cttgaacttg gtgaattaaa
    27181 aatgactttc ttgcatactc caggacatac tcaaggatgt atgtgtataa gagttgaaga
    27241 tgaaatgttc acaggagata ctttgtttgc tggaagtatt ggtagaactg atttatatag
    27301 tggagacttt aaccaaatgg aaaaatcact taagaaatta tgtaaatatg aagatgctgt
    27361 tagagtatat cctggacatg gaccaaccag tactctaggg gttgaaaaga agacaaatcc
    27421 ttacatgtaa ttttatatta gtatttaaaa aagtataaaa tatttaataa ctatattagt
    27481 gagggggttc ttctactgtt aaatagaagg agttaagttg atgttgtatg tttttttaaa
    27541 aggacatgat tataagtatg aagtagcaga acttataaaa ttgtttacta gtgaatttaa
    27601 gtttaaagac tctaatgcta acaataatat agagtcaaag catttaataa atagattgat
    27661 ttatgagaat ggtgtattat tctcaagtac tgaatactat gaaaatggaa acttaaagta
    27721 cgagtcaata cagaatatag aaaatatgaa tcttgaattt ttagatggat ttgatagcag
    27781 caaaacgatt gaagaatttc ttaatagcct agatagtgaa agtaagagga actttaaaaa
    27841 gctgtgtaaa gaaaatatca aaaaaagcat gtttttagtg cttaaacaag tatttaattc
    27901 ttatgtacca tggggaattt tgactggtat tagaccagtc aaaattgtcc acaatttaat
    27961 ggataaaaag ctagatgata atagcataag aaccattcta aaagataatt attttataat
    28021 agatgagaaa atagatttag ctcttgaaat agctaaaaga gagcgattat ttatgtatcc
    28081 tatagataaa aataaaattt ctctttatgt aagtattcca ttttgtccaa ctagatgtgt
    28141 atattgttca tttccttcaa attcattaaa acaatttgga catttaaaaa gagaatatgt
    28201 ttataaattg attgaagaaa ttaaggggtt tgccaaagtt attaaggata ctaagaaaga
    28261 agttgaaact ctatatattg gtggtggaac tccaactaca cttgatgagg aagaattgga
    28321 tttgcttata aattcattgt tcaatgaatt agatttaagt aaaattaaag aatttactgt
    28381 agaagcaggt agacctgaca ctattacaga acaaaaacta agagtgttaa aaaaacacaa
    28441 tgttagtaga attagtataa atcctcaaac tatgaatgat gataccttgg taaagattgg
    28501 aagagagcat agagtatctg atttagtaga ttgctttaat atggctcgta gaatgggatt
    28561 tgataatata aatatggata taattttagg tcttatagat gaagacttaa atatggtaaa
    28621 gaacacattg gaggagataa aaaaactttc tcctgaaagc cttacagttc atacattagc
    28681 cataaagaga gcatctaact taaatataaa tatggatagg tataaagaac atctaacaca
    28741 atatgaagaa atggtaaaaa tgatagattt atcaatgaaa tatgcaaaag atatgggatt
    28801 aaatccatat tatatgtaca gacaaaaaca tatgttggga aatttagaaa atataggtta
    28861 tgctaaagaa ggatatgagt gcatttataa tatacagatt atggaagaaa aacaaagtaa
    28921 ctatgcatta ggtgcaggtg ctatatcgaa atttgtctat gtagatgaag atagaattga
    28981 aagagtagaa aatgtaaaaa atgttgaaca atacattgaa agagtagatg aaatgataaa
    29041 aaggaagaaa gaagaggtat ataaaaatgt tgactaaagc tcctagagga actaaagaca
    29101 taactccaaa agaggcttac aaatggcgtt atgtagaaaa taaatttaga gaaatatgtg
    29161 ctttatatgg atatgaagaa atggtaacac ctatatttga acatacagaa cttttcaaga
    29221 gaagtgttgg agatactaca gatatagttc aaaaagaaat gtattctttt aaagataaag
    29281 gtgatagaga aattacccta aagccagaag gtacagcagg tgtagtaaga gcatttatag
    29341 aaaataaatt atatgcagat actcaaccaa caaaattatt ttatgtgaca ccttgtttta
    29401 gatatgaaag accacaagct ggtagacaaa gacaatttca ccaatttgga atagaagcac
    29461 ttggaagtga tactccttca atggatgctg aaataatcgc actagctgtt caatttttta
    29521 atgaagttgg attaaatgat ttggtagtga gtataaattc agttggctgt ccagtttgta
    29581 gaaaagagta caatgcttta ttaaaagagt atcttgactc aaaagctgat atattgtgtg
    29641 atacttgcaa tgagagaaga gaaaaaaatc caatgagggt tatagactgt aaaaatccta
    29701 catgtaaaga aaatatcaaa gatataccat ttattgcaga ccatttgtgt gatgattgta
    29761 aatctcactt tgataaactt caagaatact taaaggaaat gaatataaac tttgtaattg
    29821 ataaaacaat agttagaggc ttagattact atagaaaaac tgcttttgaa attatatcta
    29881 atgatatagg agctcaaagt actgtatgtg gtggaggaag atatgatggt cttgttgaac
    29941 aattaggagg accaaaaggt ataagtggaa ttgggtttgg actaggaatt gaaagacttt
    30001 tacttacact tgaaggtaat ggaattgaaa tagaaaaccc tcaatctacg gatatattta
    30061 tagtaactat aggtgaagaa gcaaatacaa gaagctttaa attgttaaaa gacttgagac
    30121 aaaatcatat aagtgcagat aaagaccata tagagagaag tgttaaagca caatttaaat
    30181 attcagataa aataaattca aaatttacaa tagtaattgg agatgatgaa cttaaaaatg
    30241 acactgcgac attaaaaaat atgaaaacat cagagcaaac tactgtaaaa ttaagtacat
    30301 tagttgaaga attaaagcaa aagctgtaaa ataagaaagt atgatgtttt gtgtattaga
    30361 taaatatata gcattaaaat taaataaaat ggaggtagta tcttggaaac tttaaagggt
    30421 ttaaagagaa ctcactactg tggagagttg agagaaaaaa acataaatga agaagtcgta
    30481 cttatgggat gggtacagaa aaaaagaaat ttaggtggtc ttgtatttgt tgatttaaga
    30541 gatacaagtg gattatgtca aatagttttt gatacagatg tagataaaga agcttttgaa
    30601 aaagctgaaa aattaggggc tgaatttgta atagctgtaa aaggaaaagt atgcgaaaga
    30661 caatctaaaa accctaatat gccaactggt gatatagaaa tatttgcaac tgaacttaga
    30721 cttttaaaca agtcagaaac tccgcctatt tatataaaag atgatgatga tgtttctgaa
    30781 gcactaagat taaaatatag atacttagat ttaagaaaac catctatgca aagaaattta
    30841 aaattaagac ataaagtaat gaatattaca agaaattact tatcaaacaa tagattttgc
    30901 gaaatagaaa caccattttt aatagctcca acaccagaag gagcaagaga ttatctagta
    30961 ccaagtagag ttaatccagg aaaattttac gcattaccac aatcaccaca attatataaa
    31021 caattattga tggtaagtgg tatggataga tatttccaaa tagttaaatg ctttagagat
    31081 gaagacttaa gagcagatag acaaccagag tttacacaaa tagactgcga aatgtctttt
    31141 gttgagcaag aagatgttat gagtatgatt gaaggtttgc ttgaagcgat atttaaagaa
    31201 gttttagatg tagaattggc acttccactt cctaagatga cttatgcaga agctatgtct
    31261 aaatatggtt cagataaacc agatactaga tttgggtatg agttaacaga tatatctgat
    31321 gttgtttgta actgtggatt taaagtgttt gctgatgcta cacaaccagg aaaaagtgtt
    31381 agaggtataa acgtaaaagg caaagctgac gactttacta gaaaacaaat atcatcacta
    31441 gaagaacatg ctaagacata tagagcaaaa ggtcttgctt ggatgaaagt aggacaagaa
    31501 ggagtaactt ctccaatagc aaaattcttc aatgaagaag aaatgaacgc tatacttaca
    31561 agaatgaatg ctgaagtagg agatttactt ttatttgtag ctgataaaaa ctcaatagta
    31621 tttgatgctt taggacaagt tagacttgaa gtagcaaata ggcttaatct attggataaa
    31681 aatgtatata acttactatg ggtaactgag ttcccagttt ttgaagaaga tgaagaaaca
    31741 ggcacattct cagcaatgca tcacccattt acatcaccta tggatgaaga tttagacaag
    31801 ttagaagaag gtgataagtc atctttaaga gcgaaagctt atgatattgt attgaatggt
    31861 tatgaaatag gtggaggaag tgttagaata tcaaattctg atgttcaatc aagaatgttt
    31921 aaagcacttg gttttacaga ggaaagagct aatgaaaagt ttggttattt attagaagca
    31981 tttaaatatg gtactcctcc tcatgctggt ctagcttttg gtcttgatag acttgttatg
    32041 ttacttgcag gaactgataa tataagagaa gttattgcat tccctaaaaa tcaaaatgct
    32101 gtttgcccta tgacaaatgc tcctacattg gcggaagatg agcaattaga agaattaagt
    32161 ataaaagttg atataaaaga taatgaataa tatgtagttg caagaaacta aaatgtaaaa
    32221 agtctggata taaaatatat ttatattcag acttttttta ttagaattat tttatatttt
    32281 ttctctatat attttcataa attatactta tattcttaac caatataatt aaaatactat
    32341 gtttaaaaat tttacataaa cttggttgtt ttagaaacta cttctgtaac gagtacacga
    32401 ttttaattaa agaatatata acaaatttga aataagtcta atatatg
//
LOCUS       DAYASV010000031        30790 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYASV010000031 DAYASV010000000
VERSION     DAYASV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.031"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            63..371
                     /locus_tag="ACGI68_001475"
     CDS             63..371
                     /locus_tag="ACGI68_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137919.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(533..2323)
                     /locus_tag="ACGI68_001476"
     CDS             complement(533..2323)
                     /locus_tag="ACGI68_001476"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137920.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(2323..4050)
                     /locus_tag="ACGI68_001477"
     CDS             complement(2323..4050)
                     /locus_tag="ACGI68_001477"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0137921.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            complement(4295..4561)
                     /locus_tag="ACGI68_001478"
     CDS             complement(4295..4561)
                     /locus_tag="ACGI68_001478"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFH0137922.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILTIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            4859..5971
                     /locus_tag="ACGI68_001479"
     CDS             4859..5971
                     /locus_tag="ACGI68_001479"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFH0137923.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            complement(6335..6844)
                     /locus_tag="ACGI68_001480"
     CDS             complement(6335..6844)
                     /locus_tag="ACGI68_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFH0137924.1"
                     /translation="MSQQNTGNVIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVVVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(6846..7493)
                     /locus_tag="ACGI68_001481"
     CDS             complement(6846..7493)
                     /locus_tag="ACGI68_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFH0137925.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            complement(7507..8679)
                     /locus_tag="ACGI68_001482"
     CDS             complement(7507..8679)
                     /locus_tag="ACGI68_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137926.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNMESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNIDKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYAIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPTICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            8947..10323
                     /locus_tag="ACGI68_001483"
     CDS             8947..10323
                     /locus_tag="ACGI68_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137927.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDEKDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDSIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILADYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            complement(10573..10908)
                     /locus_tag="ACGI68_001484"
     CDS             complement(10573..10908)
                     /locus_tag="ACGI68_001484"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137928.1"
                     /translation="MPPQIPIQWNNGVANYVDKKFIYAYPVICVVFRILLRPFIWRWL
                     QIHAFYSDTIADYLTNFMCFVALSIEIFSIFFIYNIVRHVTIIIFIDILVFIALLIIG
                     VYKLFKKYN"
     gene            complement(11259..11789)
                     /gene="bilS"
                     /locus_tag="ACGI68_001485"
     CDS             complement(11259..11789)
                     /gene="bilS"
                     /locus_tag="ACGI68_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HFH0137929.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYGIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(11877..12971)
                     /gene="bilR"
                     /locus_tag="ACGI68_001486"
     CDS             complement(11877..12971)
                     /gene="bilR"
                     /locus_tag="ACGI68_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin reductase"
                     /protein_id="HFH0137930.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            complement(12973..13419)
                     /gene="bilQ"
                     /locus_tag="ACGI68_001487"
     CDS             complement(12973..13419)
                     /gene="bilQ"
                     /locus_tag="ACGI68_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bilirubin utilization transcriptional regulator
                     BilQ"
                     /protein_id="HFH0137931.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            13725..14915
                     /locus_tag="ACGI68_001488"
     CDS             13725..14915
                     /locus_tag="ACGI68_001488"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0137932.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            15085..16047
                     /locus_tag="ACGI68_001489"
     CDS             15085..16047
                     /locus_tag="ACGI68_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0137933.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(16169..16831)
                     /gene="zmp1"
                     /locus_tag="ACGI68_001490"
     CDS             complement(16169..16831)
                     /gene="zmp1"
                     /locus_tag="ACGI68_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HFH0137934.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(16931..17016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(17202..20120)
                     /locus_tag="ACGI68_001491"
     CDS             complement(17202..20120)
                     /locus_tag="ACGI68_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HFH0137935.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     ENENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNLGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIRNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(20238..20323)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(20450..21628)
                     /locus_tag="ACGI68_001492"
     CDS             complement(20450..21628)
                     /locus_tag="ACGI68_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFH0137936.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     gene            22003..22188
                     /locus_tag="ACGI68_001493"
     CDS             22003..22188
                     /locus_tag="ACGI68_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0137937.1"
                     /translation="MFFISDIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(22253..25027)
                     /locus_tag="ACGI68_001494"
     CDS             complement(22253..25027)
                     /locus_tag="ACGI68_001494"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0137938.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(25199..26455)
                     /gene="glyA"
                     /locus_tag="ACGI68_001495"
     CDS             complement(25199..26455)
                     /gene="glyA"
                     /locus_tag="ACGI68_001495"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFH0137939.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(26527..28029)
                     /locus_tag="ACGI68_001496"
     CDS             complement(26527..28029)
                     /locus_tag="ACGI68_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFH0137940.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(28120..29310)
                     /locus_tag="ACGI68_001497"
     CDS             complement(28120..29310)
                     /locus_tag="ACGI68_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0137941.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(29549..30637)
                     /locus_tag="ACGI68_001498"
     CDS             complement(29549..30637)
                     /locus_tag="ACGI68_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137942.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
BASE COUNT        10155 a         5221 c         3623 g        11791 t
ORIGIN      
        1 gtaaaatttt ttagaagctt ctaatgttat gctataaaac tatcttgaga agggctgatt
       61 gaatgtttaa taaaatatat gaaaagaaag aatttatagt ttttcaagtt aaaaaaggtt
      121 atgtagttta taatactaga aagagttttg aagaaggtca tacacattta aagcattttg
      181 aagcagcaaa gacagctatt gatttagcaa taaataaaaa gattcctaga agcaaagatg
      241 gatattatct gactagttta attcgcataa gtgatgatgg ttattacatt gataagttaa
      301 gcgaattgtt atatgttaga gaacaaaaag gtaaaaaaga aaagtactgt aattctggtt
      361 atcagatgta acaagactag ttgtgaatta taagtaccaa tcataagtac ctttagtctt
      421 aaaatcaaag agtatcagta aatctataaa aaaacaactt actgatactc tttttaatta
      481 tatgattaat atttgaattt gatacaaaat tttatacaaa agtttatgtt atctattctg
      541 attctttgtc tgcaaattgg ctattataaa gcttctcata aaatccaccc ttttccatta
      601 attcatcatg attcccctgc tctataatac ttccattatt cataactaaa atcaagtcag
      661 cattgcgaat tgtagaaagt ctatgtgcta taacaaaact agttctacca ttcataagat
      721 ttctcattgc tttttgaagc ataagttcca gcctagtatc aacagaactc gtagcttcat
      781 caagtatcaa tattgaaggg tctgatataa aagctctagc tatggttaaa agctgttttt
      841 cccctagaga tatattagat gcttcttcat ttaaaaacat attgtatcca tctggaagag
      901 tggttataaa gtgatgaaca tttgctactt ttgctgcttg tataatttct tctttagtag
      961 ccccaaatct accatattct atattttcaa aaatggttcc attaaataac catgtatctt
     1021 gaagcaccat accaaacatt gaacgcaaat ctttacgttt catatctctt atatctactc
     1081 catcaatttt aatagcacct ttctttacat cataaaacct catcaataag tttattaatg
     1141 ttgttttacc tgctccagta ggtccaacaa tagctaccat ctgaccactt tttacttctg
     1201 catttaaatc ttctattaaa gttgaatttt ctccatatcc aaaattaaca tgctcaaatg
     1261 taacatttcc ttttacattt tctatcttaa ctggattttc aacatcaggt atttcctctt
     1321 cttcgtctaa aatttcaaat acacgttcta tagctgcaaa agaagattgt attactgtag
     1381 ataactgagt catttgtgaa agtggctgat ttatctgcca tatataacgc acaaatgctt
     1441 gtaaatttcc tactgttatt cctccactta ctgcatatat agaaccgaca actgccacag
     1501 ctgcaattcc aaagtaagtt actaatgaaa ccataggatt catcatactt gatataaatt
     1561 gtgccttaaa tccattttca caaagttctt gatttacttt tttaaattct tttatagaat
     1621 cttcttcttt accatatagt tttatttcat taaatccagt atacatttct tgtacttttc
     1681 catttaattt tccaagagca ttttgttgca atgaaaatct actttgagat ttttttacta
     1741 caaatttaga tacaaaataa ctaattggaa ttataaaaag agctataaga gccatatata
     1801 tattaattgt aaacatcatt gaaagtgcca aaattatgac taagattgag tttacaattt
     1861 gcataaaact ctgctgcaat gcatttgata ttgtatctac atcatttgat atacgactta
     1921 atacatctcc aaaactatta ctatcaaaat aactaatagg caatcttctt attttatttt
     1981 cgacttcatt tcttaaatct ctcattgtgt tttgaatagc attcgtaagt agaaaacttg
     2041 aagtatacgt aaaaattgca ttggcaaaat atatacatgc taaaatcacc aatatttttg
     2101 ttacataatc aaaatttacg gaagcccctg gtactccttt tgcgatacta attacatctt
     2161 tagtcaattg tgttattatc aatccttcag ttttaggtgc aagtgcattc atagtagctg
     2221 ctgaaattat acatataata gctataataa aggaaaattt atatttcttt acaaatggaa
     2281 gaagtctacc aattgttttc ttaaaacctt tagacttttt cattattcta attcctcctt
     2341 ttttaactga gatgttgcaa tttcatagta tatttcacag ttttttaata attctttatg
     2401 agttcccatt cctacaactt gtccttcatt gagtactata atcttatcag catcaataat
     2461 agaactaatt ctctgggcaa caatcaatac aattgcatct tttgtttcct tcttcaattt
     2521 agcacgaagt attgcatcag ttttaaagtc aagtgcagaa aaactatcat caaaaatgta
     2581 tatttcaggt cttcttataa tagcccttgc aatagaaagt ctttgttttt gacctcctga
     2641 tacatttgct ccaccttcac taatcaattc atcaaacttt ctaggttttt ttgatataaa
     2701 ctcatatgct tgtgcaactt ttgcggaata ttctaattct gactctcctg ccttatgttt
     2761 cccaaatcta atattatttg ctatgctacc agtaaaaagc agtgattttt gaggtatgaa
     2821 tcctatcttt tttcttaaag cttttaaatc atattctctt atatctactc catttatctt
     2881 aatgctacct tctgttacat cataaaatct aggtatcaag ttaattagag tacttttccc
     2941 actaccagta cttcctataa aagcgactgt ttcacctgtt tttgcagtaa atgaaatatc
     3001 cttaagtaca gaagcttctc catctggata ggtaaaagtt acattatcaa attctatgat
     3061 acccttattt tcagtatcct taattccatt ttcaggattt tttatcaatg gttcttcatt
     3121 taatagttct tttattctat ttgcagatac ctctgctctt ggatacataa taaataccat
     3181 tgaaaaaagc ataattgaaa acatggcatg gaaaagatat tccaaaaatg caaccaactg
     3241 tccaacttga agctctccaa tatttatctt ctcacttgaa atccaaaata cagccaaaac
     3301 agctatattt aatagtaaaa agaatgctgg ttgagtaatt gacataagct taaatagttt
     3361 cttagaaaca ttagcataat atgtatttgt tttttcaaat cgctctgttt cataatcatc
     3421 atttccaaaa gctctaatta ctcttattcc agttaaattt tctctcgata taaggtttaa
     3481 tttatctagt cctttttgtt gttttgaaga tattgcctga gatacttttg ctattattgc
     3541 tactccaata attataaaag gtactgatat agccaatact atagaaagtt ccacacttgt
     3601 tctaattgtc attattatac ttataatgaa cataactgga gttaataagg cagtccttag
     3661 tagtatatta acaaattgtt gaatttgaaa tgcatcattc gttgttcttg taatcattga
     3721 agaaattcca aatttattgt attcagtatg tgaaaattct tgtgatttct caaatatgtc
     3781 atttcttata tctctggtta cacttgttga taccttagca gaacaataac ccaacaatat
     3841 actacctaca acccctataa tagaaataat aacaatcact attcccattt gttttatgta
     3901 agctatattc tgatttgcaa taccattgtc aattatttta gctattatag ttggaatccc
     3961 caactcgact aatgcaaaac taaatatagc cagtatgttt aatactatta ataacttata
     4021 attctttaaa taacttaaaa ttaatttcat atttttctcc tttcttaatt tttgagatag
     4081 tgtaatcatg taataatact aaatttataa atgcttaatt ttataaaaaa aggagaaaag
     4141 agttaacgca ctctcttctc cagcatattc tttttaatac accctccgac aagtcgctta
     4201 tatgttaaca tatttgacaa gtaatgtcaa ttcatatagc caaaagtctt ttaaatgctt
     4261 cgtttcataa cattgtaaca aattggcatg taatttattt ttgtttttta ctgtattcca
     4321 attttacaag ttctccaata ttactagctt tcttaaaatt caaattagtc ttatctatac
     4381 cagacttaaa tagtgaaatc cctctcccca atattattgt caatatatat atataataat
     4441 catcaattaa gttatctttc ataaataatt ttactatatc gcctccacca actatccaaa
     4501 tatctttttt atccttctct tttccaattt gctcaattaa ttttttaggt tctaaattaa
     4561 tttatagtgt gattattcta tgtaaacatc acttttttac tcattcaata ataccctatt
     4621 aataaatcct caaactaatt taaataaact ttgtataaaa ttaaaaatat attatataaa
     4681 taatttaaaa ctacactaac ttattgaaaa ttctaattat tacaaataaa tattgctctc
     4741 tgttaacgct agttctataa tataattata agctattaat attataaagc cgatattact
     4801 aatataataa ttataatact tatagttaat ttacttttgt tgaaaggtgg taaataacat
     4861 gaaaatttca gaagtcgcta ctccaaattt tttgctagat ttagaccagt tagaaaagaa
     4921 tatagataaa attcaacaaa tttgtacaaa taataataaa caactttggc ctatgcttaa
     4981 gacacataaa agtacttaca tagcgagact tcaaaaaaat gcaggtgcta gtggttttct
     5041 agttggaaca cttgatgaag gtgaagctct gataaaagca ggttttgaag atattatgtt
     5101 ggcttatcct tacatgggag aagtaaatat aaatagaatt gtgactatgg catcaaaaat
     5161 aaatttaatc tgtgctacag ataatataga atgtgctaag gcttatagtg aagctttttc
     5221 taaatcaaaa atagagtgca agctacttat tattgtagat tgtggcttac accgttttgg
     5281 ggttgaacca gaaaaagtgg ttgaacttgc tactgaaatt tctaagctag aaaacatcaa
     5341 gattgttggt ataagttcgc atccaggtca agtgtatggt tgtagttctc cagaaggtgt
     5401 tccagatgtt tgtgttcaag aagattcttc tatggagaaa gcatacaaag ctctaactga
     5461 taatggattt aatatagaaa ttgtcgcttc tggtagtact ccaacagttg aatctgaagc
     5521 tgcttctaaa actataactg ctgtaagacc tggaaactat gtatactatg atgctataca
     5581 gataggttta ggttgtgcta ctgaagatat gtgtgctctt actgttgtgt gcactgtaat
     5641 ctcaaaaaat agcagtggat actatttaat tgattgtgga agtaagtgcc ttggacttga
     5701 taaaggtgca catggaaata gtagtattgt tggttatggt agggtatgtg gacatccaga
     5761 acttactatt gattcacttt cagaagaagt tggaaaaatt aaagtaaatg gtgaaactga
     5821 tataaaaatt ggtgatagaa taagaattat acctaatcat tcttgttcca ctgcaaactt
     5881 gacaagtaat ttattaggtt ttagaggaga tacaatagaa aaagtcatag aagtagatat
     5941 tcgtgaaaat tcaaaacaaa tcatccttta aaaacacatt tattctgata ttaacatgaa
     6001 agtatatttt tcttaaaatt attgctaata actaatgtta atgttggata aataaaaaac
     6061 ataaattcta gaaatataaa tttaaaagaa gaggctgtct caaaatagag ataagcctct
     6121 ctttcattgt tgaaattatg aaaatatact tgatttcata caaaaagagt atctcatgaa
     6181 ctaaaaagtt cactttgaga tactctcttt ttttaattaa tatacacatt aattactatc
     6241 ttagtttatt actatatatc aattactact cattaatcct tgtatatatt aattcttata
     6301 tatcatttat agttaggtta taattttatg ttatttacat taatcccata acagtgagta
     6361 aaactcttac tatcaacatc cccactattg catcaaatat aggaactgcc aacagtaaca
     6421 ttctatactt agtactggca tttgctacaa gcactattct ggcataatga ccaactagag
     6481 tacccattaa catacatggc ataattaata tggttgcttg tggtgcagtg attagacctt
     6541 gtgcaaataa gtttgcagct gtagctgctc ctgctgcctt agcaaagaat gctgctatca
     6601 aaactacaac agattcccca ggtagtccaa aaatacccat tactggtcca aaaatatttc
     6661 ctaaaatatc aactaaacca gttaattgta aaaactgcac tattacataa cccattatca
     6721 tggctggtaa tatctgttca acacctatgt aaaaaccttt ttttgctccc cccataaata
     6781 tctcaacagc agttttctta ggtttactat ttacaattac atttccagta ttttgttgac
     6841 tcatactaca cagcctcctt agccttattc tttttatttc ttacacctat gattaatcta
     6901 actagattag caccaagtat tttacagaat atctctataa gtattattgg acctaatgcc
     6961 aataatgcta ttggtaaaag tggagcttga gtatttattg tgttcagtat aacagctgaa
     7021 ccagcatatt gatatgctac aaatatagtt ctttcatcat ctgttatttc cccactttca
     7081 aataattctt tcgtcattat tgaggctaca tctgaactag taaatgaaga tacaaatgct
     7141 attcctgcta ttccaggtat accaagcaga ggtctaagca atggattaaa tatctttgat
     7201 gccgctttca ttcctcccat agattcaact acatctataa gacctacagc aaaacagaca
     7261 gttggaatca aagtcagagc aaataagaaa ccatcttttg ctcccgttcc tcctttaccc
     7321 ataaagtcta caccatctgc aatagtacca aatgaacctg tcagattagc aaaatctaat
     7381 gctttaagga agccttgact atctttaaac actcctgaaa ataatactat caatacaaat
     7441 aaagataaat accctttcca tgttacctta ttatctccag ctgttacatc catataaaat
     7501 ccccctttat acctcaaatt tatctaagtt taaaataact gtagaaatta atttcaccct
     7561 ctcaaacata gaatctacta ctgcatattc tcttgatgtg tggttccatt ctcctcttac
     7621 accaaatgaa cagattgtag gaacgtttgc aatagttaaa taacttgcat ctgaactacc
     7681 tccaagtctc ttagcattta attctccaaa tccattttct tttgacactt catttacaaa
     7741 tgtatgaaat ctcataacat cttctgtagt ttcaaatggc atcatttcac ttataaaatt
     7801 aacttctgta gaagttcctt ctatgtaagt ttctttacat atctctctaa cttgttcttt
     7861 aacattctcc atttcttcta ctttttcaaa tctaaggtct acttttatac tacaatcttc
     7921 tggaatagaa ttttccactc ttccaccctt aatcacactt acacttactg tagttccaac
     7981 atctaaatta gtcaattttt gtatgcgtag tatcttatta gccatttctt ctatagcatt
     8041 tcttccacct tcaaaatcat tacctgcgtg tgtttctaca ccttttacat gtatattaca
     8101 tcctattctt ccttttctac ctacacacaa actattatct actaatccag tttccatatt
     8161 aaaagcacaa agaccacctt ttgcttccct caatataaca tctgcacctg ttgaatcctt
     8221 atgtcctatc tcttcatctc cagaaaatac tatttttatt ggtctttctt tgtatcctat
     8281 tttatttaat gctttaatag catataagga aattattata ccacccttca tatctaaaac
     8341 acctgggcca tatgcttttc cttctattat tttaaatgga ttctctccaa atgtacctgt
     8401 ttcaaagaca gtatccatgt gacctgaaaa aattattggc tttttatcta tattggaacc
     8461 aagtgtacct atcaatgtac taccattatc tccaacatct actaactcgc aatctaaacc
     8521 ttctttttca aactcaaact ttaatctctt agccaattta tttacgcttt ctttacaatg
     8581 tgtatagctt tccatattaa caatctcttt ccataatgaa actatttctt ctctattctc
     8641 atcaacaaac ttagaaattt catttaaata tgtattcata atcttctcct ttattttcaa
     8701 ataaaatgta tttctttcct aaaatctaag tttattttag gaaatcttaa aagactagtc
     8761 cttaattaca aaatagcaca attattcttg acagtcaaca tatttttcag tcttttttat
     8821 attttctgat ttttctttca attttccttt tgtaaaataa attgacgcgt tttatctact
     8881 attataaaat gaggttataa attgattgat tagttacaat actctcgaaa ttttaaagga
     8941 ggttttatga aatataaaat tggttttttg acaacatctt attttatgtc agataagttt
     9001 aaacaagctc ttaaagaatt aaatgatact tgtgaaatta catttattga gcttaaaggg
     9061 gagaatcctt ttaataattt acctaatata tactatgagc aagtagataa ttttgatgga
     9121 tttgtatgca gtggtatagt tccatatagt gaacttataa taaatataaa agacataaaa
     9181 gttccattaa attttttaaa gcttgatgaa aaagattttt ataaatactt attcaaatta
     9241 ttaaatagcc aaaaagatat tgactttaca aaatcattta tggatttttt aagagaggac
     9301 aacaattatt atgatatata ttcattaata gactctaata aatgtccata tacaattaaa
     9361 gattttaata tccctcaacc tgtatatgac tttaaaaaat taaatgataa attattggaa
     9421 atacatttag acttattgcg agataataag attaaccttt cttttactag aaattacatc
     9481 attagttgtg aacttaataa acttggatat gattctattt acatggttcc atctacagaa
     9541 tcaatcctga atacatttaa atctctggtg aatgaaataa cattacaaaa cttagacaaa
     9601 aataaatctg caacttgtat agttacagta aatagttcag agtacataaa tgaagatttg
     9661 atatttaaaa atgatgagat acaaaatcaa atatataaca ctatattaaa ttctctatca
     9721 agacatggat tctatggagt tcaggtcaaa aaaaatgata tgaaaataga gatacacact
     9781 actaaagaaa tgttaaacaa tatgaccaat aactatacag atttttttat ttcagaagat
     9841 ttaaaagaaa ttaaatatag cttaaatata ggttggggca ttggaaatac aaatattcat
     9901 gcagagcaaa atgctcgtca agctaatggt aaatctgctt ctttgaatgg aaactgtact
     9961 tttatagtaa atgatactaa tgatacaata ggccctttat attcaaataa aaattctaat
    10021 aatattgaag atagctcaat agccaataaa gttgcttctt ttatacctct atctaataca
    10081 aatgtatcta aaatcatgtg tatgattaat gatagaaatt caaataatgt gtcagcagaa
    10141 atattagcag actatatgaa cataactctt agaagtgcta atagaatatt gagtatctta
    10201 tataaagcag gaattgcaac tattgtaaat acaaaactcg ataatcaaag aggaaggcca
    10261 aaaaaaatat ataaagttga tttcttatct tttctaaata agatgcagaa attaaattct
    10321 taatatttaa ttttttaata aaagcaatgt attttctcta attttaaaga atcaaaatgt
    10381 ttaaatttta ttagaatata caacttttta ctttaaaata cctaaacctt ttatcagtga
    10441 aataaaccat aatatatcgt aacttattag aaaaacaaaa taatttcata tacaaataat
    10501 aaaatagcca ctacaatcca taatttgcag tggctacttt ttacactatc acttattact
    10561 tatttttaaa aattaattgt attttttaaa taatttatac acacctatta tcagtaaagc
    10621 aataaataca agtatatcta taaaaataat tattgtaaca tgtctgacaa tattgtaaat
    10681 aaaaaatata gagaatattt ctatagacaa cgctacaaaa cacataaaat tcgttaaata
    10741 atctgcaata gtatcactat aaaaagcatg tatttgtaac catctccata taaatggacg
    10801 taaaagtatt ctgaatacaa cacaaatgac agggtaggca taaataaatt ttttatctac
    10861 ataattagct acaccattat tccattggat aggaatttga ggtggcaact tttgaaatac
    10921 tgttattgca atttaaacac tacctctcta ttctattaat ttataactat aatatagtct
    10981 taaatatttt cactctataa aattgtatat ttagtatatc ttaaaatcaa actaattatc
    11041 ttttataagt tttcatacaa taatatctcc tttcaataat atatcttttt ttatatttaa
    11101 aactatctta ttttctataa aataatgtat aaacaaggtt tacagtcaag tatttttatt
    11161 atttttttga tttcataaaa aaagcatagc aaattatttg ctatactttt ttactaaatt
    11221 atttattcta tatcttatat gaacttattg tttaactact acacaatact agctttaatc
    11281 atcatatttt ttgcaaacat actggcttca tatatttctt gttcatctgg atgactttga
    11341 gattcctcat ggttatttaa ttgttggcga atatgttcgt tgtcaggaga aagttttaac
    11401 atctctttat attttgcttt caatccttct gctatcttac cttgacatat gaatgaacct
    11461 agtatttcat tatcttttgg gataattgat tctatctttt ctattatact agctccataa
    11521 cttccttttt tacttgctcc cattgttcca aatagtgcta tctttttatt gtgaatattt
    11581 tcaagcactt gttttgaatt tttatcacaa attcccttgt ccacccaata gcctaagaat
    11641 actatatcat aattttcata gtcaatacca taatcaacta attcatttac atctttaatg
    11701 tctttttctt cttgaatttc atcatatata gtataagcaa tctttttagt atttccagtt
    11761 aaacttgaat ataaaactaa acttttcatg atgtctcctc ctctttatct aaatatttat
    11821 ctaaatcacc aaaaatatat taaccttaac aaattattta tttttataag gatattctaa
    11881 tctagtatac atccaaactg agagcgtgtt tgtaaagcat ctgcacactt cttattacaa
    11941 taacagcact ttttaatctc atctactctt ccatctttta ctttctgtaa ccaattacta
    12001 tctgctagta attgtcttga catagaaacc atatctactc tattatttga aataatactt
    12061 tctataaaat ctgggctaga caattttcca accccacata ctggaagatt tgtgtgtttc
    12121 tttacttcat cagctaaata taaaaaacag ccttcttctt taaagtaagg atgattattg
    12181 gttggtatag tatcttctaa ttttgaatga tttgcaagtg ttacatgaaa actgtttaca
    12241 cctagggatt caaatttttt aacaaaatac tcaacttccg aaagtaaaac tcctgcattt
    12301 ccataatgag gattttcttg tcttattgca aatttatagt ctattggaat gtccttaact
    12361 tcatctcgga tactactaat tatttcgctt gcaaatctac ttctattatc acacgttcta
    12421 ccatattcat catttctatt attaaatata cttgatgaaa aacttcctac aagcctatca
    12481 ccatgaattt gaatcatgtc aaaacctgct ttttttgcat tcaatgctgt tactttaaat
    12541 aaatttataa tatttttaat ttcttctttg ggcatatttg ttatgtaatc ttttactccg
    12601 tcattcatta ttttttttat ctcatcatgt gaagtaattc ccatattcat agtatcttta
    12661 attaaattaa catcataatc tgaacaaaat agttgtgctg atacctttgc accagaattg
    12721 tgtatttcat ctacaatttt cttgtatttc atgactccat cttcatccga taggcttata
    12781 gctaccttat gaaaactagg tctaatagat acatctccta gagtaatcag agctactcct
    12841 gacttagcaa tatcagaaaa ctttttaatt ttttcttcca atttaagacc catgcttgta
    12901 ggtgcaaaag atactctatt ttttaattcc aaatttccaa ttttaattgg ttttaataaa
    12961 ttacaataca tcctattctt cctccacagc tgtttttata tcctttacca catgtaaatt
    13021 ctttaccagt tctttatatt ctgtatccga gaactctttt tttatgtgat tatcccactt
    13081 tgaaaatatg ttttttatat cccctaatac ttcctcaccc ttatctgtta ctctcaactg
    13141 aaaagctcta ctatcattag ggttttggat tttttctatg tatccatctt gttctaacct
    13201 agatatagtt cttgttgtat gagctttatc aacttcaaat tctgtagaaa catcattcaa
    13261 agaacaattc ctatttttat taatataaat taaatagaaa tatagaccat tcgtaagttt
    13321 atatggtttt aaacattttt cacaataaag tttaaagttt ttttgcaagt tacaaataat
    13381 atatgacaaa ctttcatact gtaaattttt aaaatccatc ttaaatttcc cttctataat
    13441 tataatatgt ttgactatat caactatatt atattaaaca attaatatag tcaagaatat
    13501 tttattctct aaatctatgt tttattaatt ctttaaaaaa ctattatata aaataatcat
    13561 gataaaatta gaaattggaa tttttaatat gtactgtaca taaatatatt ttatataaaa
    13621 gtttatattt tcaaacaagt aatttttcga aaattgtcta aattcagtaa aaatatggta
    13681 atatatacat aagcaatata aatctataaa aaggagtgtt acatatgata tctaataaaa
    13741 tgcaaacttt agttgcaaat agttctgtta taagagctat gtttgaagaa ggaaagaaac
    13801 tttctgatat ttatggtgag gaaaatgttt ttgattttag tataggaaat ccaagtgttg
    13861 aacctccaga gactattaaa gcagttataa atgatatttt aaatgaagaa tctccaaatt
    13921 tagttcatgg atatatgaac aactcaggat atgaggatgt aagagatgct attgctgaac
    13981 acataaataa aaaagatgga ttaaatctta caagagaaaa tttaattatg acttgtggtg
    14041 ctgctggtgg tctaaatatt attttaaaaa ctcttttaaa tcctggtgat gaagttattg
    14101 cttttgcacc gtattttggt gagtataaaa attatacaga aaactatgat ggaaaattaa
    14161 tagaagttcc tacaaatata gagacttttg aaccagattt agatgctcta aaaaatgcta
    14221 ttactccaaa aacaagagct ttaataataa acactccaaa taatcctact ggtgtcatat
    14281 attctgaaga attattaaaa aatcttggag aattattgga ttcaaaacaa aaagaattta
    14341 atacaagcat atacctaatt tctgatgagc catatagaga aataatttat gatggtgcca
    14401 aagttccttg tgtactaaaa tactatagaa attcatttat aggatactct tatagtaaat
    14461 cattatctct acctggtgaa cgtataggat atattgttgc taatgggcaa atggatgatt
    14521 ttgatgatgt tatgtcttca ttaaatgttg caaatagaat acttggattt gtaaatgctc
    14581 cttctctttt tcaaagggtt attgcaagaa gtttagatgc agaagttgat gtaaatattt
    14641 ataaaaagaa ttttgacctt ctatataata gtttaatcga tatgggatat tcttgtgtta
    14701 aacctaatgg tacattctat ttgttcccaa aagctccaat tgaagatgat aaaaaattct
    14761 gcaatgatgc aaaacaattt aatttattat tagtaccagg ttcttctttt ggttgtccag
    14821 gtcattttag agtatcatat tgtgtatctt atgataaagt taaaagttct ttaccagcat
    14881 ttgaaaaatt agccaaatta tataatttaa aataactaaa tcaaagtaat tcaaataatt
    14941 aactacctta gtatagaata cttacattct atattaaggt agtttataat tttatgtact
    15001 atattttata cataccatat tattccaata tgcttgaata ttcttatcta tagtactaat
    15061 ataaattaca aggaggtgtt tgacttgaaa aaaggttatg tttatataat actgactacc
    15121 ttatttttta gttcaatgga aattagtcta aaaactgtca ctaatgactt taatccctta
    15181 caaataactt tatcaagatt ttttgttggc ggtttagtct tactaccttt tgctgtaaaa
    15241 agattaagag ctcttagtct ttctattaca aaatcagact taaaatactt tgcattttta
    15301 ggttttatgt gtgtagtagt aagtatgtca ttgtatcaac ttgctgttct aaacacaaag
    15361 gcatctgtag ttgctgtttt gttcagttgt aatccagtct ttgtaatgct tttagcttac
    15421 ttaattttga aggaaaaaat atataggcac aatgtaattt cacttatatt agaagttctt
    15481 ggaattatag ttataataaa tccattgcat acaaaactaa ctcttagtgg aatattctta
    15541 acattatctt ctgctattat tttttcaatg tatactgtat ttggtaaaag aaaaacctta
    15601 aaatttggtg gaatagttgt aacctgcttt agctttatat ttggtagttt ggaaatgttg
    15661 atattggttt tactcactaa aattaatttt atcgcagata tattaaataa aaataagctt
    15721 catgtatttg caaatatccc tatttttagt ggatatacct tacacaatat gcccattatg
    15781 atttatgtat tcgtatttgt tacaggtgtt ggttatgcgc tttactttat ggcaatggaa
    15841 gctacatcta ctagtcttac atctttagta ttcttcttta aaccagtaat atctccaata
    15901 ctagcattat taatacttaa agaaattatc cctataaaca tggttatagg aattttattg
    15961 atagtaattg gttcaataat ttctattata cctacgataa ttactcaaat gcataataaa
    16021 cacgttgaag ccttgtataa ggagtagtaa ttgataatct atcatagtta tacataagac
    16081 aaaatttatt agatttaaac gcaatataaa agagatgcta ttataatagc atctctttat
    16141 ttatatttat tttaaattta ttaaaactct atttagctaa attttgcaaa aagctataag
    16201 tttgtggaca agctgatttt aacttactat ttgtatcttg atttaaatag taatatgcaa
    16261 atgattctgc aaaaaattct tctggatata cacctaagta gtttacatta cctaaagaac
    16321 gaccttcttt agcaaatatt tgtttgaatt gtgctgattt agatatatca tttaatacta
    16381 tatggtctat tgcatgtgct gtttcatgta attctaaatt tattgcatca tgccctttac
    16441 ctttgttact aaatcctatt ctaagagcta ctgtacttcc acctaaacct ggaacatcat
    16501 cccaagtttt tccagtacct tcccatccct taggaactac accttttaaa taagcatatt
    16561 ctttttcatc tgttaacttt ccacttaata attttatttt taagttattt tgctttaatg
    16621 catttaaata ctttccatct atattagcta atctatttac catagcatta gcttcgtttt
    16681 tatcgtaatt tccagttgga aaaactacta tttgactaag tgtgtcttta ttttgttgta
    16741 tagtagtact atctgcatgt gcacttactg gaactgaact tattaagaat attgatatta
    16801 tagctattaa taatttttta cttggtctca tattattccc tcctttgagt taggccggga
    16861 gtaataaatt tcaaaataaa aaagataccc aagagtatct ttgtatatac aaaaagaaat
    16921 ttttactctt ttcggcaact ggctggcatg gtttactaaa ctttagcccc tatagctttg
    16981 cgtgactagg tttccctagt tttgcctcat ttaattttat atctatatta tagcttatat
    17041 ctttttaatt gtctatatcc ctttggaact tttttctaaa aagttattat ttttttacat
    17101 aaatcttcaa aaaaaaatat cttctaacaa tattttcatt attaaatatt gttttagaag
    17161 atatttttta atataactta tattttttat aatgttaggg tctaatttgt atttttattt
    17221 cttcttaata cgataagtcc tacaatagct cctataacca aaagtatttc tccaataatt
    17281 gtagtactat cccctgtttt tggtggtact ggtggattta ccaaagtatc atctttaact
    17341 ttattatttt tttcttctga cactgatggc tttactactt ctggtattgg aataatctgt
    17401 ttaacaggag tttctatcat ctcttctgat ggtggtactg gcggattcaa tacctctggt
    17461 attggcggat ttattatatc ttctgatggt ggtattggtg gatttataac tatagtatcc
    17521 tctgtatcag atgaacttga tggtttcctt ggtttatctg tgcttggtgg tactggtgga
    17581 tttactattg gttcatctgt atttggtggt gctggtggat ttactggtgg attttcttgt
    17641 cttatgtttt ctatatttcc aatatccact attctaccaa tatctttatt accaatttca
    17701 accttaattt tatccttcag tttttcatat ccacttggag ccattatctc cactaaataa
    17761 tatgttcctg gtttttttat actagattga attataccat cttcatcact aactaaatcc
    17821 ttaactacta tatttccaaa tgcatcttct aaattgaact tagcacctga aagtacattt
    17881 ttatctaaag catccacttt tttagcttca aaagcataac cttgcttagt agtctctact
    17941 cctggaggac ttgaaccatt aatctgagcc acatttcgta tagtctcccc aacttttaca
    18001 tttgtgaagt ctgcatcaaa tgtaacccaa ctttgcacag gttccccttc ttttatctgt
    18061 ttaaattcgt atttatttac taatcttatt gtattttttc catctccagc ttctttttta
    18121 agttcaagat tatcagaata ttttatatta gatatttcta gagctgagtt tacttcatct
    18181 aaaagattta cttgtcctgc atcataaacc ccataagttg acatattgat tttgtattta
    18241 acaatctggt tatcactact atttaaaact tttttatcaa cttctttttt tgcgcttatt
    18301 aatccagaat ttttcttaaa gtttatctca tcatttttat caatttcact attatttgca
    18361 tcaagaacag ctctatttat atagctatct tcaatactaa ttacctttaa tgtataagct
    18421 ataaaaaact ttgattccac ttcttcttta ctgagattaa ttgtaaatcc agttataact
    18481 ttatctactt gcccactttc atttcttttt tcttctactt tatatgaaat attattggca
    18541 attttttctt tactccaagg agtccagctc ccattcattg gaatttctac aactctaaaa
    18601 tcatgcccag cttctcctga agtagttaat ctcatgcctt ctggtatatt atctgttata
    18661 acaacattac ttattggtga tttatcatca tttattgaaa tctgatagtt tattacttga
    18721 ccaacttgag taatatttgt tgctcctgaa tgtcctttag taagaagata atcatctcct
    18781 ctatcaaata tacttacagt agcatcatct tcttgtatct tcttaccatc agattcaatc
    18841 ctagctacat tcttatattt attaccatgt tttgcatttt tgtcactgat tctagcctta
    18901 tacttaacag tatacgattt atctatattt ccaagattaa ctgaaataga atttttactc
    18961 tgttcaatat taaaatcact aacttctttt ccatcacttg ttactacact tacagaacca
    19021 tttatcaatg tcaatccttc aggaagctca tcatacaaaa taatatcttt taactcttcc
    19081 tgcttttcat ttacttgtat actgtaattt atatctttat ttttaactgg cataaataca
    19141 gtagttgatt tttttccttc aacaagcttt ttaagtgttc ctttagcatc tggaacatcc
    19201 gttttatctc caggttttat ttctacatct atatctccac ttccactttc tatttttcca
    19261 gtagtttcaa tatgtatttt tgcattagta tcttcatcaa cctttttaac atatgcttcc
    19321 aaacctattt taccttgcat tacactttgg ccattaggta attctctaaa tgtaagtgta
    19381 tactctccat tactttctgt tttatcaaag cattcacttg tataatctaa attaacttta
    19441 ttgaatacat ctggcacctt aaaagttatt gaatctcctg cttgtattcc ttttgatgtt
    19501 ggagtataag ttaaattaaa tgatattttt ttcattgatt ccgtttctat tgtattgtta
    19561 ttaattttta caacaaatcc atagtcttca ttttcttcat taagttcttc ttctaaaact
    19621 ttcaataaat tttcattttc taatagatta tcttcctctg gagttttttc tgtattttta
    19681 ttgtctagtg gtttttcatc ttctaaagct tgttctgact ttttattatc tgttgactta
    19741 tcttcttctg gtatctgctc tggcttttta ttatctattg gcttttctcc ttctggagtt
    19801 tgctctggct ttttattatc tgttgactta tcttcttctg gggtctgttc tggtttttta
    19861 ttatctgttg gtttttcttc ctctggggtc tgttctggtt ttttattatc tgttgcctta
    19921 tctttctctg gagtctgttc tggcttttta ttgtcgcttt ccttctcctc tccagttgat
    19981 gaattagttg aaccactttg aatctgacta ttctctccta attccgaagc ataagataca
    20041 gttggatata ttgtagacac caccatactt aaaatcataa ttaaactaat taataatgcc
    20101 tttctatttc ctttcttcat gtaaatctca cccctttagg ttacctataa tttataatct
    20161 aatcttccat taatatcgta tataaagtaa tttttactaa aaaattatac aaaaaaagct
    20221 atgaaaataa ataaatttat attcatagcc ggttgcacca ctgactcagt atatccataa
    20281 ggtgcccaat aatagaatat acttcaacat ctctataata tttcataaaa acaattaaaa
    20341 ttgctttatt tatattaaaa atacaattat atacttctat tatattatgc ctctatacta
    20401 ttgtctatcc tttttacatt ttttacctta tgatgagatt aattaataat taaataaaat
    20461 ccttatctac aacaattaca caattatatt caggatttat ctccttaact aattttgtta
    20521 tatcttcttt taagtcatct tctgacatgt ttttggaaaa ttcggaagga ttaactacta
    20581 tatcaaatat taaattcttc ttgcttcctt ttccaactat tctaaaatca tgcatagatt
    20641 ttacaagact atttttctta agtatttctt caagttcttt tttggctgac ataacttctt
    20701 tatcttcaag acatattggg tccatgtgaa ttactagata tatatctaat ttttcagata
    20761 tttctctctc tgcagtatct ataatttcat gtatttccat tatatctatg ttggatggaa
    20821 tttctgcatg tatagatgca atacatctac caactccata gttatgaata attaaatcat
    20881 gtgtacctat tatatattta taagataaca atagctctat aatagaattt accatttctt
    20941 catccggtgc ttcccctaaa agaggattta tagtatcttt tacaagagag aaaccagcat
    21001 aaagtatcac taatgatact acgataccaa catatccatc tatgggaaaa ttagtaaatc
    21061 tagctacaat aaatgatata actacacaac ttgaagtaaa tacatctcct aaagcatcaa
    21121 gtgaaactgc ttttaaggct gaagaatcaa ttttatttcc cataaattta ttaaaacgac
    21181 tcaaccagat tttaatcatt atagatgcaa ttaaaagtat aagtggtatt acttcaaatt
    21241 ttacaggaat tggatttact atcctttcta tagatgactt tacaaattga agtccaacca
    21301 acatcaccat aaatgctact attaatgctg ataaatactc tattcttcca tgaccaaaag
    21361 ggtgttcttt atcagctggc ttacttgcta atttaaaacc tatcatagtt attgctgaag
    21421 atgccatatc agaaagatta ttaaatgcat cagccattat agctatacta gatgtgagca
    21481 ttcctatgaa tacctttatc acaaataaaa gtaaattgga taatatacct actacaccag
    21541 ctacataacc atacttattt ctaacatctg tattttgaac gttttcgcta tctctgataa
    21601 atgttttgac taggattttg gaaaacatga aacttacctc cctgcccttt attttattaa
    21661 caaattttat aatctatatc tttatttatg ataacataat ttatatataa aattcttaaa
    21721 aatcactcta cttcagtaaa atattattct atatatctat ttagatacga attttatatt
    21781 tagtaaagtt tttatggctt agtgtctgtt taatagattc tatataagtt tttacaaaga
    21841 ttatttatta tgataatatc atttttccca agtattactt agaatatgat ttttttgttt
    21901 tgtctgtaca tattttaaat ttatactata tttatctata ttttataacc ttatgataca
    21961 ctataactat aagattttaa atggaaagga atgaaataaa tcatgttttt tatcagtgat
    22021 atttacacaa aatcacctat taaatttgat actcctttgc aaaaagaggc atataaaata
    22081 ttacaaaaat tagatattga ctttgaatgt gttgacacag atgaagcaat tacaatggaa
    22141 gattgtgttc agattaataa aaaattaaat atgaaaatgg taatataaaa caacattaaa
    22201 caaacaaaaa ccgcaaagtt acaatatata gtactttacg gtttcataag aattaatttt
    22261 tactattaaa gttcatagtt ctactatttt tcattgttct aagaattagc tttacaaatt
    22321 catttgacaa taggatagtg gatgcaaata gtattatctt tatccacata tcaaagtcca
    22381 aagaaacaga attaaagaaa ctctggaaca cttgtgtaaa tattatttga attatccctg
    22441 taactactat tatttgcaat gccagtttat ttttaaagaa atttggtatt gtactattta
    22501 aaccaaactc tctacaattt aatgcattaa aaagggcact gaatgcaaat aaagaaaata
    22561 taacagtacc ttgttcttct gatgtagctc ccaatatatt aaaagctgat tgagtaaata
    22621 caattgtaat tataagaatt gcatttataa taatatttac aaacatagac ctagctatta
    22681 ttccagaatg tctatttatt ggttttcttt tcaatacata atctctaaca ggttctaatc
    22741 ctaatgctag ggctggagga ccatccatta ttatatttac ccataataat tgtattgtag
    22801 taaatggcat atccttacca gtaagttgag atattatagc aattataaat gctactatat
    22861 ttacagtaag ctgaaactgt ataaatcttt gaaagttctc atatatacct cttccccatt
    22921 ttattccctc tactatagta ctaaagctat catcagtcaa tattatatct gctgcatttt
    22981 tagatacttc tgttcctgaa atacccattg caattccaac atctgcttga ctaagtgctg
    23041 gggcatcatt tataccatca cctgttacag ctacaacttc gccacttttt tgaagtgctg
    23101 aaactattct catttttgta tctggtttac ttcttgcaac aatagatatt cctttgattt
    23161 cttctctcaa ttctttatct gtcaatacat ctatatatgt tgcttcaact gcctttttcc
    23221 catcatttaa taatcctaat tcatttccaa tagctgttgc tgtatttata ttatctcctg
    23281 taagcatttt tacatctact cctgcattaa atgctttatc aatggaatct ttgacccctt
    23341 ctctcaatgg gtccactatt cctacaaatc cactaaaaac taaattatct tccttcatat
    23401 aacttcttga atttcctatt aaattcaatt caccttctga agtcattgca acttctgttt
    23461 tagaattact cattttttta taagcaaatc ctaatgttct catagattta atttgtaact
    23521 tcttaatctc atctaaaata cttttttcta cttttggagt tattggaaca atattttttc
    23581 cttgttgtac ataagaacat ttttttaata atacttctgg agcacctttt gaaagtaata
    23641 tatctgaatc ctcttgacga attaatgttg acatcttctt ttcttctgaa ctaaatggta
    23701 tttgtgatat caaatacgtt tgttttctca tttcattata gttcttatca ttatgatata
    23761 aaagtaatgc acactctgtt gcgcttccta tatatttaaa tgaattatct tctttttcta
    23821 tatctgcagt tgaatttact atacagtttt gttcaaaata gctattactt tgatactctc
    23881 ctccacttat atatttacca tcaacatatg ctacctctac catcattttg ttttgtgtta
    23941 aagtccctgt tttgtcggaa catattacag aaacagaacc tatagtttca caggcttctt
    24001 tctttgttac taatgcattt atctttgcca tcttttgcat tgttatagct aaggtaatat
    24061 ttatcatagt tggcaaacct tctggaacag ttgcaactat taatgcaata caaaccatat
    24121 atgcattttt agcaggttct aatgattgta agaaagataa tatactgctt gtatctaaaa
    24181 ttaacatacc ttttctctgc atctgtataa tcataaacat acacaataaa gtagctatta
    24241 tgcctgatat tcctgctatt ttagcaccca acttccctaa tttaatttga agtggagttt
    24301 gctggtcttc atttgataaa ttctgtgcta tcttacccat ctctgtctta tcaccagtat
    24361 gagtgacaac cattatgccc ctaccatatg ctacaagagt ccctccaaat accatattga
    24421 cttgctttgc aggtattgca tcctgttcga ttatttctgt tttagaataa actacttcca
    24481 tatctaatac agcatcagca ttcttagcta catcatcaga ttctcctgtt agcatatctt
    24541 ctctaagttt caaatttata gactgtataa gtcttccatc tgctggtatc atatccccag
    24601 tttctatata tacaatatct cctggtacta aatcattttt tgatatttta attatcttcc
    24661 catttctaag cacctttact tctatatttt ctgttaattt tgacagggca tgagctgctt
    24721 ttttagattt tccttcagtt attatgccta tagaaattcc aataaaaata gcccctaaaa
    24781 tacctaatgc gtcatgtaat tctcccacaa aagaacttat tactgctgct cctatgagaa
    24841 ttaatatcat aggttctgtc aaactttctc ccaactcgtc ccaaaatgtt ctaccttctt
    24901 ttatagtaaa ttcatttaat ccatatctaa gcttcctttc ttctacctca ttatcatcaa
    24961 gtccaatttc tggatttgtt tttaagtatt ttaatacttc tttggtaggt ttattataat
    25021 acctcataac tttatccccc ttgttaatta tctataagga attatatgct tatgatagct
    25081 tgtatattac ttttcaaaaa aaataaggtg ttgattaatc aacaccttat agtggttata
    25141 tattattaaa ttttatgtta caggctttat aaattgtgag ctatacaaat caaaatattt
    25201 aatcttcaaa atacccttct ggatataatg gaaacttaga tataagttct tgtgcctctg
    25261 ctttacattc atctaatact tctttattat caatattttc tgctacctta ttcattatcc
    25321 ctgctatctg tataacttct ttttctttca gacctctttg agatattgat gtaagaccta
    25381 tacgaacacc actagttaca aatggtgatt gtgggtcgtc aggtataaca tttttattaa
    25441 ctgttattcc tacatattca agagcttttt caaattgttt tcctgtaaga ttcttacttc
    25501 ttaagtcaac cattactata tgattatcag tacctccact tacaatacta aaaccatgtg
    25561 aaatcagttc agaagctaga gtctgagcat ttactacaac ttgctccatt atctttttaa
    25621 atttatctgt acttaaatat aatagtgaaa aggcttttgc tgcaactgta ttaagatgta
    25681 tagaacctaa tgttcctgga aaaacacctt tatcgagttt ctttgcatgt tctgctttac
    25741 aaagtacaat accacttctt ggtccacaga aagtctttgt cgtagatgaa gaaacaaaat
    25801 ctgcataagg tacaggacta ggtattactt tcgctgcgac taatcctgca acatgtgcca
    25861 tatctaccat aaagtaagct cctacttctt ttgcaacctt tgatattctt tcataatcta
    25921 ttaatcttgg atatgaactt gcccctgcaa ttataagttt tggtctacat tcttttgcct
    25981 ttgcttcaag agcatcataa tctatacgtc cagtatttgg gtctacccca taaaattcat
    26041 atttataaat gtcatgtaaa aaattaactg cgctaccatg tgtcaagtgt ccaccttggt
    26101 caagacgcat acttaaaaca gtgtcatttg gttttaatat tgatgaatat acacaatagt
    26161 tagctgtaga acctgaatac ggttgtacgt ttacatgttc tgctccaaat acttcttttg
    26221 cacgttttat tgctagggtt tcaagtttat ctgcttcttc agaacctgct tgaaaacgag
    26281 cccctggata gccttcttct gttttatttg taaatacaca tccacttaat tcaagtactt
    26341 ctgttggtgc tgtactttca gaagctatca tctcaatatt gtgttcttgt ctaaccaact
    26401 catcagctac aatcttgtat aactctgggt ctgaaatttt tgttgttttt aacataataa
    26461 tcctcctctt aattaaagaa tatattttat tagtttaatc ttcattgata aatttattat
    26521 aacggtctat aaaacgtaag atattcctcc accaggtcct aatggtatgc caatacccat
    26581 gaatagtaac aatattatta tttctattaa tgtaaatacc actgagtatg gaatcatatt
    26641 cgatatgaca gtacctatac ctatatcttt atcatatctt ctggcaaagg ccaatataac
    26701 tggaaaatac ggaaaaagtg gacttattgg atttgttgaa gcatctccaa tacgataagc
    26761 tatttgagtt aatgctggat taaagtctag tagcataaac atgggaacaa atataggtgc
    26821 taaaatcgcc cattttgcag aagcacttcc tacaaataaa ttgataaatc cagatagcaa
    26881 tataaatcct attatcaatc catagctagg tattcccgac tctgctagta attttgctcc
    26941 tgttattgat aaaattgttc ctaagttact aagattaaat aaattaataa actgggatgc
    27001 tataaatgct aggactatat atccacccat atcactcata ctactaccca tcatagatac
    27061 taaatctttg tcacttttta tcttcttact tacttttcca tacactaatc ctggagttaa
    27121 aaatataata gtgattatag gaactatacc tttcattaaa ggtgcattac tagatagtat
    27181 agaaccagtc tctggatctt tcataaatgc attgtcacct atacataagg caactatcac
    27241 tactactaca actaacaatg ataataaagc gtattttata cccttatttt caagttcact
    27301 caattcctgt atatccttat catttgttga atcattatat tttgtaaaac gaggagctat
    27361 aaatttttca gttacaaatg ctcctgcaaa agttaaaact atcgctgata taaaagcaaa
    27421 ataaagattc attgctggat tgccttggta agatgggtcc atcatttgag ctgctggtat
    27481 tgtaaatcca gctaacaata catctgtagt actaagcata acatttgctg caaatccccc
    27541 tgcaacacct gcaaaagctg caaacatacc tacaagagga tgtcttccaa ttcctaaaaa
    27601 cacaagtgct gcaagtggtg gtaatattat aaatcctaca tctgctgctg tatgtgacaa
    27661 catacctgca aatacgattg ctacagttac aagtttttgt ggaactttac ttataccctt
    27721 tttcattaac acttccataa atccagattt ctcagcaaca cctgctccaa gcattgtaac
    27781 cagtactaat cctaaaggag caaatgactg aaagtttgta actatgctac ctaaatatga
    27841 ttgaatctgc tctttctcta gtagatttac tacctttaca acttcttttg tagaaggatg
    27901 tatttcttct actcctaaat tagatataac tgctgacaat attacaacaa ttatacatag
    27961 taataaaaat atggttactg ggtctggaag tttatttcct gctctctcta caacatttag
    28021 tgccctcatc aataatccac tatctttgtt tttttcctgt gaattaattt tcctcaaact
    28081 aataccccct cataaacttt atctccagaa tgctctatct taaattttat tttttaattt
    28141 atccatataa tttataacag actgacatag tacatttgta cctaccaata tagaattttt
    28201 atcagcttta aatttaggat tatgccactg atatatacaa tcttgttctt tgttgcctac
    28261 ccctaaccag taaaaaaatc ctggggcttt ttccatgaat atagaaaaat cttcccctcc
    28321 tgtagaaggt attggatcaa ctatagcatc ttctgaatac aaatcacaca cagacttaca
    28381 agctattgcg tacatctctt tactatttat aacagctggt aaatcaaata aataatctag
    28441 ctttgcagtt gcttgatatc cacgagctat ctcacaaact atattttcta aattttctga
    28501 tattttcttt cgtacttcat tactaaagct tctacatgtg cctgtcatat ctaccttttc
    28561 tgaaattaca ttatttgcag tccctccatt tatagtacct attgatataa cagctgattc
    28621 taatggactt atgtttctgc ttactatggt ttgaataccc attataattg cacttgatgc
    28681 tactataggg tctattgttc tgttgggtat acctccgtga cctccaaatc catttacttc
    28741 tatcttaata gtgtctactg ctgccattaa cccacctaac ttaacaccta tttttccaca
    28801 aggtatatct ggatgattat gaagtccaaa tatagcatca acttttggat tttccattac
    28861 tcctcgttcc actagcatct tcgcaccttt attgacttct tcggctggtt gaaatataaa
    28921 ctttacattt ccatgaagac tatctctaat atgagacaat acatatgcac atcctacaag
    28981 gcaagacgtg tgtatatcat gcccacatgc atgcattata ccatcatatc tggatttata
    29041 cttaatatca acctcttcat atattggcaa tgcatcaata tctcctctta gtgctactgt
    29101 gggaccatca tacttccctt ttaataatcc aacaactcct gtttccattc ctaaatctaa
    29161 tatttctatg tctaaactca ctaataaatc ttttatagct tttgtagtcc taaattcttt
    29221 aaaacttaat tcggggtgca tatgaatatc tgtaaaaaaa tctaaaatat cttctttata
    29281 cttttctgag attaaaataa gttcatgcat actaacacct ctccaaaatt ttattttttt
    29341 attttttata atttaactac aaattatata agttaataat ctttataaag atatttttta
    29401 taaatttaat tttagtttat atttatattt tactatatta ttttaattaa atatattatc
    29461 atattttaca atttatttaa ctattttgac tttttaaatt cggattatat aaaaaatttc
    29521 tttaaataaa aactattaat aagtggctct attcctgact ttgtgttgat ttttttctac
    29581 atgtaattgc tttatacacc atacagacta aaattaaaag ccccatataa cctgtatatg
    29641 gatataaaat gtttactagt ttaccaaacg gtaaaaaacc tcctatacaa gccaaaatag
    29701 ctattactgt tgttaccaat ttaaatttag gatgttcatc ttcaacaatt ctattggtta
    29761 cagaccataa aagtggaaca gctgttgtgt agatacctaa tattaatgca actgaaaaac
    29821 atacacctac aataggagat atattatctg ctaaataaag tgatggtatt tctttcatat
    29881 acaaatttcc tatatctgaa atcatagcca aatacataac tataccagca gtcataaaca
    29941 ctattccacc taataaacct ccccaaaaag catccttttt actttcaata gaagctccca
    30001 ttcctgttaa aaaaacaatc attacaataa tatttaatgt attatatatt acaccagaaa
    30061 cgattggact ttctgatgct tttgtaactt caatattttt catagcttct ccaaccatag
    30121 ataaaccatc aaaatgctta aacaagctta ttaatccaac agcaattgta aatactgcta
    30181 tcactggtcc aatacgtcca agtatgctta ctaatttttc taaacccata gacactgtaa
    30241 ttaaagtagc aacagccatc aatactcttc ctaagtaagg atttatacca tagtattcag
    30301 ttaaagttga gcctgcacct gaaatcatta ctataagaat cccaaataaa aatattggac
    30361 taaaccattc gtaaaatttt cctaaaatct tcccacaata aaaagtgtaa attcttgttg
    30421 gttctttaag ttttaattct ctacctctag ttataacttc agcaccaaga aatgaaaaca
    30481 ataccattga tattaatgct ccaattaaac ccatataccc ttgtgaggca aaaaattgta
    30541 gaatttcttg accagtagca aacccagaac caattgctgt tgctagataa gcgcctgcaa
    30601 tacagataat atttttcata ttcactttgt ctgacatatt aaattacctc cattataaag
    30661 ttaccttcct ttaagaaata taaactctca gtaaattaaa tgaaaattct gtctttttga
    30721 aacagtattt aattcacttt ttttattttg tatatctttt tctttttatt tatatatatt
    30781 tttttatata
//
LOCUS       DAYASV010000032        30607 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYASV010000032 DAYASV010000000
VERSION     DAYASV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30607
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.032"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            435..1127
                     /locus_tag="ACGI68_001499"
     CDS             435..1127
                     /locus_tag="ACGI68_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0137943.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1323..3383
                     /locus_tag="ACGI68_001500"
     CDS             1323..3383
                     /locus_tag="ACGI68_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0137944.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3588..3911)
                     /locus_tag="ACGI68_001501"
     CDS             complement(3588..3911)
                     /locus_tag="ACGI68_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFH0137945.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3904..4605)
                     /locus_tag="ACGI68_001502"
     CDS             complement(3904..4605)
                     /locus_tag="ACGI68_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFH0137946.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4883..6268)
                     /locus_tag="ACGI68_001503"
     CDS             complement(4883..6268)
                     /locus_tag="ACGI68_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0137947.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6376..7614)
                     /locus_tag="ACGI68_001504"
     CDS             complement(6376..7614)
                     /locus_tag="ACGI68_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0137948.1"
                     /translation="MELFIEVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLIV
                     VRMMFISKINLNILNINNTTINIYKHNIDNYIPYDQGITLDIFMYIWILGIIAISFYY
                     LRTNFKFYNYIENFKKEILEIDITSILDEQAKELSINKKLQIYKVKGIYSPAIIGLTR
                     CKIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIYWFNPILYLLKKYFH
                     ELCELSCDEIVIDKCTTSEIKEYAYIILDTIKYQKSLKYSSFVSQFLNKKNIILKKRL
                     DSMFNQDKKKINLGLKFLLTFIIIGSIFSINSNFKQNEDVEYTIGKINYNKNGETYGT
                     HIIDSNGNSIEPELIQTIGRGKKVGYVKKRDLYDCDNQPKTPEEAVLYTRKRNFNIFK
                     KTIPLYDKEGIKIIGRFEIN"
     gene            complement(7627..8004)
                     /locus_tag="ACGI68_001505"
     CDS             complement(7627..8004)
                     /locus_tag="ACGI68_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0137949.1"
                     /translation="MIILKKIPKSELLVMNFIWDKDIDKIPAREVANYMEISHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTLYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCGNEISDDDIKELEQWLKKRK"
     gene            complement(8523..8945)
                     /locus_tag="ACGI68_001506"
     CDS             complement(8523..8945)
                     /locus_tag="ACGI68_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFH0137950.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            9423..12326
                     /locus_tag="ACGI68_001507"
     CDS             9423..12326
                     /locus_tag="ACGI68_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0137951.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEEDEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKISDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIITLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDESLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            12359..12805
                     /locus_tag="ACGI68_001508"
     CDS             12359..12805
                     /locus_tag="ACGI68_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0137952.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGDMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            12928..13230
                     /locus_tag="ACGI68_001509"
     CDS             12928..13230
                     /locus_tag="ACGI68_001509"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0137953.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            13411..14817
                     /locus_tag="ACGI68_001510"
     CDS             13411..14817
                     /locus_tag="ACGI68_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0137954.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPVITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            15161..16219
                     /locus_tag="ACGI68_001511"
     CDS             15161..16219
                     /locus_tag="ACGI68_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0137955.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVNIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMEKGDIIFKPMISDKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            16318..17004
                     /gene="araD"
                     /locus_tag="ACGI68_001512"
     CDS             16318..17004
                     /gene="araD"
                     /locus_tag="ACGI68_001512"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFH0137956.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            17022..17669
                     /locus_tag="ACGI68_001513"
     CDS             17022..17669
                     /locus_tag="ACGI68_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFH0137957.1"
                     /translation="MLDSLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIASYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(17842..19209)
                     /locus_tag="ACGI68_001514"
     CDS             complement(17842..19209)
                     /locus_tag="ACGI68_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0137958.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(19399..19487)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            19915..22035
                     /locus_tag="ACGI68_001515"
     CDS             19915..22035
                     /locus_tag="ACGI68_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFH0137959.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKICNRLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRLFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            22139..23392
                     /locus_tag="ACGI68_001516"
     CDS             22139..23392
                     /locus_tag="ACGI68_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HFH0137960.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSQMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVIAMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(23546..24607)
                     /gene="ytvI"
                     /locus_tag="ACGI68_001517"
     CDS             complement(23546..24607)
                     /gene="ytvI"
                     /locus_tag="ACGI68_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HFH0137961.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            24881..25621
                     /locus_tag="ACGI68_001518"
     CDS             24881..25621
                     /locus_tag="ACGI68_001518"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HFH0137962.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            25633..27411
                     /locus_tag="ACGI68_001519"
     CDS             25633..27411
                     /locus_tag="ACGI68_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0137963.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            27578..28495
                     /gene="pfkB"
                     /locus_tag="ACGI68_001520"
     CDS             27578..28495
                     /gene="pfkB"
                     /locus_tag="ACGI68_001520"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFH0137964.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     IFKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            28532..30451
                     /locus_tag="ACGI68_001521"
     CDS             28532..30451
                     /locus_tag="ACGI68_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0137965.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVTEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        12085 a         3358 c         4978 g        10186 t
ORIGIN      
        1 aatttaaata agtagctatt attgcagtga aatgaatgtt tctaagtata tttgggtgga
       61 agaatggtgg aagaaaatta gtgaaatgta attcttccac cattcttcca cctagaaaat
      121 agaaactgtt taataagaat taatatacat taattcagct gaatgcaatc aaaatactta
      181 aatgaaagta atctgaaaac aacataattc aaatcaattt attcaaataa acctcaattt
      241 atcttaaatc ttaagaaata tttaagaatt aggataatat tttcttaaga tttatatttt
      301 attatatagt tataagggaa agttacaaaa atgtaagcca aagtaatata acaatttagt
      361 cattgttgag aaagggagtg aaagatattg tataatatag ataagtatta ttatacaata
      421 gaggaagtga aaatatgtac aatatattag tagtagacga tgataaagaa attgttgatt
      481 cgatagaaat atatcttaaa tcagaaggat ttaaaatata taaagcatat gatggaatga
      541 aagctttgga aataataaca gagaatgaca ttcatctaat actaatggat ataatgatgc
      601 ctaaattaga tggaatcaaa gcaactgtaa aaatacgaga agaaagaaat attccaataa
      661 ttttaatttc agcaaaaagt gaagatacag ataaaataat aggattaaat attggagctg
      721 atgattatat aacaaagcca tttaatctat tagagcttat agctagggtt aaatcaaact
      781 taagacgata tgtaacttta ggtaattatg aagatacatg taaagaaatt ctaaaaagtg
      841 gagctttgga gttaaataca ctaacaaaag aagttaaaat tgatggtgaa aacataaaaa
      901 tgactccaat tgagtataaa ataattgaac ttcttttaga aaataaagga cgagttttct
      961 caatagatga gatatacgaa aaagtatgga atgaggaaag ttttaattca gaaaatacag
     1021 tagcagtaca tatcagacga ataagagaaa agattgaaat aaatccaaaa gaaccaagat
     1081 ttttaaaggt ggtgtgggga gttggttata agattgaaaa attttagtgg aaaaattata
     1141 aaagaaaaga gtttaatatt taattgcatt ttgattttag tcatttttac atttacacaa
     1201 atgttaggaa ttttaatatg ttttaatttt ttctctgaga gtttaattta ctctatgggg
     1261 attatgcttt tcttaaactt tattttatat aaaatactga caatgtagat gaggtgggca
     1321 acttggagaa atatagagta ttaaagttag tttcattaat cataatatct atctcatttc
     1381 cattaactat attgttggtg acaataggac ttgctgagcc taaatacaat atacaaaaaa
     1441 cttctacaaa gagtgggatt tttgatatat ctgatgattt ttatgatagt aatatatttg
     1501 acgaaggaac aatggcaagt ctattaccca agtttaagct atctgtaggt ggtaaattaa
     1561 ctagtaaaga aaaaggtaaa ttccaacaag aaggtaaaag tatagattta agtagtagtg
     1621 attctgagta tgataaaatc atgattaatg aacaagcttc tgaagaatat ttaagttatc
     1681 taaaaaatat aaaagtgtat gttattaata catacacagg agtattttat acaaatacgt
     1741 catatgctag tttatatgat tttaaacaaa aaactaagag ctattgtaat gtagagataa
     1801 taaataagat aggaaagagc tcatatatta aaacaataaa tggagaaaaa tttgaactga
     1861 ataatctgag tctggaacta aatgaagtag atgaaggttt tgaggcatat gtatcttttc
     1921 cagaagagcc aactatagaa gatggaatag tatacactaa ttttcaaata tttaaacaag
     1981 ctacagaaaa agtaagatta ttttttgtag ctagtttaag tttagcaatt atatgtttat
     2041 tatctataat tttatatatc aaaataaaag gtgaagtttt aaaaagaaat aatgtatttt
     2101 tactaatata cagtaagata cctatggaat ttaatgtaat attagtatct atttcaattg
     2161 caattttaac tatatatgct cttgctgtat cttatcatat tgatgtaatt gcattagttt
     2221 ctttatcttt actgatagta tcaaataata tattttttat aaatgtgcaa ttgagctatt
     2281 tagaaaataa aatgtgtata tttaagacaa gtattatagt aagagtgatt ttatggttaa
     2341 taaaaactgt aagggaggtc agtaacgcta taaagaaagt atctttagca aggaaagtta
     2401 ttgttatagt gataatatct ttagctttaa tcatggcaat tagcactcga agtataggta
     2461 tgggattact gttttctata tgtatttttt taggatttac aatatatata actaaaagac
     2521 tttcatatct tagttacgta atagaaggta cagaaagaat aaaaaaggga gaattagatt
     2581 ataagataaa aattactggg aatgataatt ttacgtcttt agctgaaaat ataaataata
     2641 ttggagaagg tcttgataga gctatagaag agcaagtaaa aagtgaaaga atgaagtctg
     2701 accttataac aaatgtatct catgatttga aaactccact tacttctata ataaattatg
     2761 tagatttaat taaaaaagaa gaatcaatac aaccagagta tataaatgat tatataaatg
     2821 tacttgagtc aaagtcaaaa agactaaaat tattgataga agatttattt gaagcgagta
     2881 gagtaagtag tggcaatata gaattacaaa ttagcaagat tgatttagtg caactattaa
     2941 gacaaagtat aggtgaactt gaagaaaaat tatctaaaaa taatttatat ttaaaattaa
     3001 atgttccaaa tgataaggtg tatatatggg cagatggaag aagaatgtat agagtatttg
     3061 aaaatctttt atctaatata gcaaaatatt ccttagaaga aactagagtc tatattgatg
     3121 tatatgacta tggtgaaaat gtaaaggtaa ctatgaaaaa tatatcttct tatgaattaa
     3181 attttgaccc aagtgagatt atggaaagat ttaaaagagc tgatgaatca aggaatactg
     3241 aaggaagtgg attagggctt gctattgcaa gagatttagt agctttacaa ggtggaaaat
     3301 tttatataga aatagatgga gatttattta aagcaaattt agagtttcaa aaatatgaag
     3361 aagaaataac aaatatggaa tgagatagta tttaagtata tatatttttt tactgtcgaa
     3421 agcttttatt ataatatatt agaatattta cagtagagaa tgaagagtaa aaacagagta
     3481 actttaagtc actctgtttt ttatttaaaa tcaataatat aacaacttaa attaaaattg
     3541 tacacatagt tttaaaataa cttatacaat aaaacattat ccaaatatca caagataatt
     3601 ccaaataaat atatagatat cactcctgta ataatagaaa taccaataga tttaaactta
     3661 tatgcaacaa gaagtacaat cataaaagat ataattttag ggttagctat agataaatat
     3721 agactatctc cattaaaaaa tacatcttta gcagttaagg aagtaagtat tgctacaggt
     3781 ataaatttca tgattttttt caaaaaatca ggcatctctt tttttgaaaa aattaacata
     3841 ggaagcactc ttggtagata tgtaacaatg tacattccaa ttataataaa aaatatataa
     3901 ttattattca taagattcct cctttttaca gtaagattct tctataaaga aaccaattaa
     3961 agatgctaaa attgatgcaa ctattattac taaattgctt ttgaataatg catataaaat
     4021 aacagaaata atgattgatg atataaatac tacaatataa atcttatttt ttatttgaga
     4081 aactattaaa gctataaaca tagaagtcaa tatgaagcca gaaatagaac tgtctataga
     4141 tatactttct cctaaaaatg ctccaataaa attagctaaa actgatgaaa ttaagcagaa
     4201 gaaattaaga taaatagcat ttctatcaga ccaatttcca gcagtaaatt tttcataatt
     4261 aactgcaaaa gtttcatctg ttatagtatg acaaaatggc aatataaatt ttggactttt
     4321 attttttatg tacatgttca aattagaact cattagaaaa tgtcttaagt taactataaa
     4381 tgtagtcaaa acaatagatg ttacacttgc accagtagaa atcattgaag ctgcaatgaa
     4441 ttgagaactt cctccaaata ctaaagttac cattagtgct atttgaaaag gtgaaagacc
     4501 agatttttgt aatagtattc ctcctactat acctataggt atataaccta agaatatagg
     4561 aattgaagca ataaagcttt cttttaattg ttgagttctt tccacaataa atcactcctt
     4621 ttgtattaaa tgtattaaag tattcgtaaa acagaaaaat agattaattg tataattgca
     4681 attataatta aaaataaatt tggattaata tataaaatac tatacaacaa atagataatc
     4741 ttaaacggtt attaaatttt atctatttgt tattatgatg taataataca tattaatctc
     4801 tataagttaa ttactatctg gtgtaatttt attttaacaa caaaaaaaaa tacacacaat
     4861 taaaatattg tgtgcaatac aattattttt tctcttctaa tagtcttttt aatacgctca
     4921 aaccagcttt aagttcttct gtactttctg tagcacatac agctattctt attgcagaaa
     4981 ttggtttttc ttttccaaca gcaaaacgtt cagaagcata gacctgtaca ccttctttaa
     5041 gtgcttgtat ctcaaatttt tctgccatta ttccatcagg tagaatcaac catctaaata
     5101 tacattcttc atttcccaaa agattatatc cagataaata ttgattaata atcttgtttc
     5161 ttcttcttgc agatttacgg cgtgcattaa ttaatttatc ggcttcacct gatgtaatca
     5221 tgcgataagc aatttctgtt aaaaaataag attgagatag attaatgttg tataatgcgc
     5281 ttgataatgc ctctcgatac tgtttaggtg tagagatata tgctagccgt aaactaggtg
     5341 caataatttt tgataaactc gtaatataaa ttgtttgatt gggcagatag gaagccactg
     5401 gatttaaatg tgtttcatta agtaaactat gaatagcatc ctcaataaca attaaattgt
     5461 atttagatgc aatatttgca atcatttttc gtccattttg tgacattata tgtgtagttg
     5521 gattttgata atctggtatt atgtatagac ctttaatatt ttcattttta caagcatata
     5581 atagtccttc ttcactaatc tcattatctt cttgtttaat tggaattagt tgaatcccaa
     5641 gcattttagc tgctgtcttt attccaggat aagttaatgg gtctacacct attctatctc
     5701 catgttgaaa caaaccagct aaaatagcaa caattgcatt ttgtccacca cttgcaggaa
     5761 gtaagctatc tgatgtagtt tcaagcccag ctttataaat taatttggca atagcttctt
     5821 tttgccattt agcaccatct ctttgtccat attggaatat ggttttaaaa tctatttcct
     5881 taattatgtg tttaaacaaa gtattcattt cttctagtgt gaaatctgga ttcattgtgc
     5941 ccatttcaat aaatgtaatt ttattattac tggacatcaa gaatagattt gaacgtgtat
     6001 cataagatac aaaagttcca cttcctgtaa ctccacttat tagacctttt ttttcacaaa
     6061 ttttaaaagc tctagagata gtactcacat taacatctaa gaaatctgcc aattcacgtt
     6121 ggggtggtaa tttagtacct ggtaataaaa atccattgtt gatatcttgt tctagttgtt
     6181 ctgcaagcgc cttatataat atttgccctt ttgaaggtct ctttggtttc cagttcatag
     6241 gataattttc aaaagaatta attggcataa gttttcacct cacattatta atttgatttt
     6301 ttctttatct aaaacaataa attctaaaga tacaaataaa aactgatagg aaataattct
     6361 atcagttttt gtcttttaat tgatttcaaa tcttcctata atttttatac cttctttatc
     6421 ataaagaggg attgtttttt taaatatatt aaaatttctt tttcttgtat ataaaacagc
     6481 ttcttctggt gtctttggtt gattatcaca atcgtacaaa tctctttttt ttacgtagcc
     6541 gactttttta cctctaccta ttgtttgtat aagttctggt tcaatggaat ttccatttga
     6601 gtctattata tgtgtaccat aggtttctcc atttttatta taattaatct taccaatagt
     6661 atattctaca tcttcatttt gtttaaaatt ggagttgata gaaaatattg accctattat
     6721 tataaaggtt aataaaaact taagacctaa attaattttc tttttatctt gattaaacat
     6781 agaatctaac ctctttttta aaataatatt ttttttattt agaaactgag atacgaatga
     6841 tgaatacttc aaagattttt gatactttat tgtatctaat attatatatg catactcttt
     6901 aatttcactc gtggtacact tgtcaatcac aatttcatca caagataatt cacataattc
     6961 atgaaaatat ttttttaata agtataaaat tggattgaac caatatatga tacaaattat
     7021 actaattaga agcttaaata agttatcttt tcttttataa tgaattaatt catgtttaaa
     7081 gatgaatctt aactctttat tattgtactt tttttctgga attattattt tacagcgtgt
     7141 taaacctata atggcaggag aataaatacc tttaacctta tatatctgaa gttttttatt
     7201 tatacttagt tcttttgctt gttcatctaa gatactagta atatctattt ctaaaatttc
     7261 ttttttaaaa ttttctatgt aattataaaa tttaaaattt gttcttaaat agtaaaaaga
     7321 gattgctata attcctaata tccagatgta cataaatata tctaaagtaa tgccctggtc
     7381 atatggtata taattatcga tattatgctt atagatatta atagtagtat tattgatatt
     7441 taatatattt aaatttattt tagatataaa catcattctg actactatta aaagacatat
     7501 aaaataatta aacttaaaag tatatttgct tatcttttta gtaaataaaa tgaaaaaaag
     7561 aattagtgga cttatgtaaa tattaaattt aataacttct ataaataatt ccataaaata
     7621 cgtctcctat tttctttttt ttagccattg ttcaagttct tttatgtcat catctgatat
     7681 ttcatttcca caacctaatg atgatataaa gctatgaata gaatttttat gtataagctc
     7741 taaaaaatcc tttgtttcaa attttagata ttcctcatgt ttaactttaa tagtgtataa
     7801 agtatttcta ccatcttttt caaattctaa aaaaccttta tcacttaatc tagaaagtac
     7861 tttaccagta gtaccttttg tccatccatg agaaatttcc atataattag ctacttctct
     7921 ggctggtatt ttatctatgt ctttatccca tatgaaattc ataactaaca actcggattt
     7981 tggtattttt ttcaatataa tcactcacct ttaacttgat tatattagac aaatgtctaa
     8041 tagtcaatat aatatatcat catgtataaa taatctttat attattttat gcttgagaac
     8101 taagtagtat agatttatag ataaaaaaga tattgagagt tttatggaag aggggaatat
     8161 attttctatt agagataaga atatgacata tattttttat tgatttttac aaaaatacta
     8221 tatagtgaat aaattaatat cttttctaaa ttagaagagg agaatcttat tagataaaga
     8281 ttatcctctt tattgaaagt ttaagggata ttagagatta ttaatttttc tctctttttt
     8341 taactaaaag caataataca taaaattatt attgctaatg atactcttaa tgtggtatat
     8401 agtcatagtg tcacatgttt atcaatagtt ttttcattaa ttattgtaac gaatagtagg
     8461 actcataaaa tacttagaaa tatgttttta gatagatttt aaatatataa tctaacttat
     8521 atctataaat taacactatt attctccaat tccatttcta gtttttttat aaatttatta
     8581 aattcttctg gttgcctttt tccaaacaat actcttacaa gagctgaatt gatttttcca
     8641 aatatattcc tcttgctctc actttcaata aaaataagtt ttgtattttc taagtcaata
     8701 tgtactaatt catatctagt aatagtactt tctcttttat tgcttgtttt tatctcatat
     8761 actctatttt tttcgaaatt agttatttca accttaaatc ttttattatt tttttgctgt
     8821 acaaatgttc ctactgcttt tttctcatta aaattaggaa aatttagtct tgcagatttt
     8881 gtaaatatat taaatatgtc ttttgcagaa tattttaata aaacttcagt tttaaatgta
     8941 ttcatatcgt tttcccttct gtatataaat ttaatattta taagttaatt tttaagttaa
     9001 aatagtataa tatgccatat taaatcatgt taaaactatt tatatagcta aaatcttacc
     9061 tatataagta tgtatactat catataaaaa taaaatctta aaatagaaag ggttaaaaac
     9121 acagtttatt ataattttta ttgttgagta gatacgtact attatatatg attgagcaag
     9181 tttttcaatc ataaatttat aaagtatttt gcattaaaat ttaaacaaag aattaaatta
     9241 tttaagatta tgaataacta atacatttag taaattacta aatatacaaa atgtaaatac
     9301 ttatcaaaat gtataaatac ttatgacatc tagttactta cagggtttaa gtagaaaaaa
     9361 atatcaaagt aagtatggca tgatagttgc ttatatattt atcaagaaag gagttattaa
     9421 gcatggagtt gagagataaa cttgtaaaca tcataaatac agaaaataaa aaagatccat
     9481 taacagatgt tcaaatagca aagtttttaa gtacaactag agagaacata actaatttga
     9541 gaaaagaact taatataagt aattcaaggc aaagaagata tccttattta aaatcagcaa
     9601 tttcggcaat attacaaaag aataagaata tttctatatc agaaattaca agggaattga
     9661 tgacagaagg ctttaatata tcgagaagag ttgtggagga acttttacct aaagaaagtt
     9721 tagaaatgga tgtagtagag gaagatgaag aagaatcgaa tgacccattt gaaactctga
     9781 taggtaataa agggagtctt agaaatgctg tagagcaggc taaatctgct atattgtatc
     9841 caccaaaagg gttaccaacg ttgattattg gggaaagtgg agttggtaag agtttatttt
     9901 ctagacatat gtatgagttt gcaaagcaaa aaaagatagt aaaagagagc tccaattttg
     9961 ttgtatttaa ctgtgctgat tatagtgaca atccacaatt attattatct ttgttatttg
    10021 gatataaaaa aggtgcattt acaggtgctg aatatgatac accaggtctt gtagaagaag
    10081 ctgatgaagg agttttattt ttggatgaaa tacatagatt gcctccaaaa ggtcaagaaa
    10141 tactattttc tatcttagat aggggaaagt ttaggagatt aggtgaaact aatgctgaaa
    10201 gaaaagtaag tattatgttg attggggcaa ctactgaaaa tgtagagact aatttattat
    10261 taacattcag aagaaggata ccaatgctaa taactttgcc accattacat gatagattac
    10321 taaaagagaa aatagacctt atatacaata tctttcaaca agagtgtaat agaataaatg
    10381 caaaaatatt tgtagataaa aatgtgattg aaatacttgc actaaaaaag tttagtggaa
    10441 atataggaca attacaaaat atgattcagg ttctttgtgc aagagctttt atgaagttta
    10501 taaattccaa agatgaagat agtgaatcta tagtagtagt agatataaat gaagttttaa
    10561 agcttaaaga ttcgtttaaa gatgctgcct ttcaagagat tgaatataca gagataagaa
    10621 aatatttgaa aaatgctata tttataccat ttaatttaga agaatcatca gtaaaaggaa
    10681 gtcttttaag tgatgaatat atattaccag aggacatata caaaacaatt gaaaagaaat
    10741 attatgacct aaaatcccta gacattgaag atatagatat agagaatatt ttatggactt
    10801 ttattttaaa tagatttgct gacttaaagt ttaatcttga tagtaatgaa gaaatttttt
    10861 caatgagtga tttaaatagt tttgtaagtg atagtttagt taaactggta aaagaactta
    10921 tgaatgatat agttgaaaaa aatatgaatt tagaagtaaa taaaaatatg ttcaaatatc
    10981 tagcaataca cttagaagaa gctataaaaa gaattaagct aaatcaaaag ataataaatg
    11041 taaatttaga aaaaataaag attgactttt caaaagaata tgaaattagt aaaagttttg
    11101 caataaaaat tgaggaatca attggtatta agatttcaga tgatgaaatt ggatttataa
    11161 cgctctacat aaagtctgct ctaaaaaatg aagtgaaaaa agataaagta gggttaataa
    11221 taatttctca tggacgtata gctacagaga ctgtaaatgt agttaaagaa cttttaggtg
    11281 taaagtttcc tgtggctata gatatgcctt tagatgaaaa accaataaat atatacaata
    11341 aggcagtaga attgtcaaaa ataatagacc aagggaaagg catattgttt ttggtggata
    11401 ttggttcact tacaaacata ggtcaaatag taaataagag aaccaatata agtactaaaa
    11461 ctattgatag agtagacctc ctaatggcac tagaagcaac tagaaaagtt tctatgggag
    11521 aggaagaact tgatgaaata tttttttctt tgctaaaaga taggatggga tataactatg
    11581 aattagaaaa acacatagat aaatcaaatg caattataac attatgtctt actggagaag
    11641 gaactgctaa gtatataagt aaaacgttgg aagaaaagta tgataacact aaatgttatc
    11701 aaatgagtgc attggatgaa agtttatttt gtaaaattga aaatctaaaa gagacaaaca
    11761 atatattagc tattgttggg acaataaacc ctaagatacc aggtataaat tttataccct
    11821 ataataaaga acttttcaaa aatttggata tctatctatc aaaatcaaaa caacaagatg
    11881 acagaccaat taaatcgtat gaaagaatgc ttgatgagga tttagtgatt tttgaaccag
    11941 atatatattt taaaaaggat ttattagaat atgtatgttc aattcttata aataaagaat
    12001 atgtagaaaa agattactta gattctgttt tacatagaga agaaatgctt ccaacatatt
    12061 ctaaaggagc tataggtgtg cctcatggag attcttctac tgtaaatagt acaaggtttg
    12121 tttttgttaa actaaagaat cctattgatt ggggtgtagg taatgtaaat ttcatattta
    12181 tgccagtttt tcaggctaat gataaagaga ttgttaaaaa tgttcttgag atactaaaag
    12241 atgcagaatt tatgaacaat gccaataagt gttttgataa agatgctttt agaaggatta
    12301 tatttgataa atttaaacat ctgtagccat agcaaaaaaa ttgagaggag ttagagtaat
    12361 gaatgtagat ttaatatcat tgggtttaga atctaatgat tatgaaagtg ttattgaaga
    12421 attaggaagt ataatgtgta aaaaagaata tgtaaaagaa acatacataa atgctgtctt
    12481 ggaaagagaa aggacattac caacaggtct agatattgga gacatgtgtg ttgctattcc
    12541 acatacagat tcaaagcatg taaatgagtc taatgtagct gtagggatat tgaaaaatcc
    12601 tgtaaagttt aattcaatga tagacccaaa agatagactt gatgtagaac ttgtattttt
    12661 attagcagta aagaacccag actcacaagt aaaactgtta aaagatttaa tgtcagtttt
    12721 tcaaaatata aagttattaa aaaatataaa aaacgcatca acaaaagaag aagtagctaa
    12781 attattggat tttatagaaa tttaatgtag gagaatttta taaataaaaa attaaaattt
    12841 aaacaatcaa agtaagtatt atttttttaa taggtttaat cttatataaa ataaaaattt
    12901 agagtgaaaa attaggagga aattattatg aaacaattta atgtattatc agtttgtggt
    12961 tcagggacag ttacatcatc tatggtagca ggtaaattaa aagaagtact aggtaataag
    13021 gggatttcta ttactacaac agaagctaga ccaacagaag cactaaattt agcacaatca
    13081 ggaagatttg actttataac atttacgagt ccacttcaaa caggcgatta tggaatacca
    13141 actataaatg catttgcttg cctaactggt ataggggaag aggcattctt tgaagaagtt
    13201 ttgactgtta ttgaagggat agataagtaa gttttggaga cgtgaattta tagtatttaa
    13261 atcaatgatt tagatactat aataagctaa tattaactaa atatttgtgg aaaaaacatt
    13321 ttatatgttg tcataaatta aaaaattata aaaatagtca cttaagtatt aaattaagct
    13381 tataaatatt tataagtagg agggaaaaag atgttagaat ttatgaaatc tataatcgat
    13441 acatttggtt ctgcaattat agttccaatt ataatattca taatcgcaaa gatatttaaa
    13501 gtaactacta agaaatcgtt tctttctgct gtgtatgcag gagttgcttt acaaggtttt
    13561 acactgattt taaattcatt tacaccagta ataacaccag ttatcaatag gatggttgag
    13621 agtacaggag taaacctacc agtttttgat gttggttggc aagcaacatc attggttgca
    13681 ttttcaacaa gtgcaggtat gatatattta ggtcttggta tattattaca aacaatttta
    13741 tttttgataa aatggacaga tgtgtttcaa ccatcagatt tatggaataa ctattcttat
    13801 atggtatggg gagcaatggt tataggagta acaggaaatt ttcctttagg tatagcatgt
    13861 atggttttat taaacctata tagtttatta atatcagagt tagttgcaaa gagatggtct
    13921 agttactata gatatcctaa ttgtacaata atagctatgc ataatgttga ggcttctgtg
    13981 tttgcagtat ttgcagaccc tatatacaat aaattgggat taaataaaat aaaattaaat
    14041 ccaaaagaat tagagaagaa attagggttt ttaggtgaac caattacttt aggtcttttt
    14101 ttaggtatgt ttataggtat attaggtaat atgacgagaa taaatactat ggaagcttgg
    14161 ggagaaataa tgaaagtagg tatatctaca agtgcagtga tggcaatttt ccctaaagta
    14221 gcaagtatgt ttgctcaagc atttgcacct ataacagaag cagctagaaa gattatgcaa
    14281 aaagctggaa accgtgagtg gtacatagca gtaaatgatg ctgttggata tggtgaacct
    14341 gcaactttaa tatcaggtct tatattaatt ccaataatgc ttgtaatagc tatggtactt
    14401 cctggtaata aggtattacc agttgtggat ttattggcaa ttccatatat ggttcaaggt
    14461 ttagttgcca tacataatgg taatatacca aaagtattag tttcaggtat aatttggttt
    14521 ggattaggat tatatgtttg tacatctaca gcaccattat ttacagatat ggctacaaat
    14581 ataggtgtag ctatacctgc aggagcaatg ttaataacaa gttttaatat actaggaaaa
    14641 cctttgatgg gattagtttt ctttgcattt ctaagtgcaa atccaattta tataggtctt
    14701 tcagttgtaa tttactttgt tctatgggca ttgtttagaa agaacaaaac aagtattctt
    14761 gattatttag aaaaacaagc acttaaaaat gtagaagaag agcctgtagc agtttaagtt
    14821 tataaaaatg aaataaaaat aattaaagga gtttataatt gattttatat aagtatcaga
    14881 taatacctga gaaatattta aatgaaatat agacttcttt ttatttgata atatttgtaa
    14941 ttataatgca ttgtattttt atagttgagt tttctgtata acctgctatt taaaacatac
    15001 tcttttatat ttatacagta atgtggttta attttataaa atgtaatctc tactggtaaa
    15061 agactttgta tataaggcat ttgcctgaat aaaccattta tcttttgtat ggcatagata
    15121 ttgctatata gaagtatata gcatgaaagg agatatttat atgggaaata aaatgagagc
    15181 atcagtattg tataatgtag gtgatgtcag atatgaaatg gtaaatatac cagaaataac
    15241 agatacgcaa gtgcttgtaa atgtaaaata tgttgggata tgtggttcag atttacctag
    15301 gtctatggta agtggtttga gtggaaatac aaaatatcca ttgattttag ggcatgaatt
    15361 ttctggtgaa gttgttaaaa ttggagaaaa agtaaagcat attaatgtag gagatagagt
    15421 agctgttgca ccattagtac cttgtggaaa atgtgattat tgtaatgaag gtaattttgg
    15481 cttgtgtgat gattacaata taattggaac aagagtaaat ggagcgtttg cagaatatgt
    15541 aagagttcca gaagaacata ttttaaaact accagataca ttggattatg aaactgctgc
    15601 cggaattgaa ccagcaacta tagcttatca tgggatttca aagtctaata ttagagtagg
    15661 agatagtgta gttgtgttag gatgtggtcc aataggtcag tttgttatac aatgggcaaa
    15721 ggtgtttggt gcatctaaga ttatagcagt tgatattttt gatgaaaaat tagaactttc
    15781 taaacttcta ggagcaaact atatacttaa ctcaaaagaa gtaaatgtaa tcaaagaaat
    15841 aaaaaaaata acaaatggtg gtgctgatgt tgtaatagaa actgctggta gccgatttac
    15901 acaagaacaa tctctatttg tagcaaaaaa gagaggaaat atagtgtttg taggtatatc
    15961 acacacagaa ttaccactta gtgcagacgc tactgagtgt atattacgtg gagaacttac
    16021 cttgaaaggt tcttggaatt catatacaag cccttatcct ggaagagcat ggactgcaac
    16081 attggatttt atggaaaaag gtgacattat ttttaaaccc atgatttcag ataaaatagg
    16141 tcttaatgaa gtgggtgact ttttaagtaa aatgagcaag agagaaatta atttcaataa
    16201 aatcctagta gaaatataaa aatttaataa aaaattctaa aagcaattaa attttataac
    16261 tatgacatta aataaaataa aaataatttt cttaaataaa atataggagg aagaaaagtg
    16321 ttagaaaatt taaaagaaga agtattaaaa gcaaacttag aattacctaa gaaaaagcta
    16381 attacttata cttggggaaa tgtaagtggg atagatagag aaaaaggatt agtggtgata
    16441 aagccaagtg gtgtagagta tagtaatatg actgtagaag atatggttgt agttgattta
    16501 gagggaaata tagttgatgg caaattaaga ccttcttcag ataccccgac tcatctagtt
    16561 ttatataaag agtttgaaga attgggggga atagtacata cacattcaag ttgggcaaca
    16621 atttgggctc aaataggaaa atctatacca tcttgtggta ctactcatgc agattatttt
    16681 tatggaagta taccatgtac cagaaaaatg actagagaag aaatttgcaa tgaatacgaa
    16741 aaggaaactg gaaatgtaat cattgaagaa tttatgggaa gaaatccaat tcattgtcca
    16801 ggaataattg taaatgacca cggacctttt acatggggaa aagatgcaaa tgaggctgta
    16861 cacaatgcag tagttttaga agaagtcgca aagatggctt attatacaga gttaatgagt
    16921 cctgataata ttatggataa ggttcttatg aataagcatt tttcaagaaa acatggaaaa
    16981 aatgcatact atggtcaaaa atgattatct ggaggtgaat tatgctagac tctttaaaag
    17041 gtaaagttgt ggaaatagca attaaagcag agcattatgg tttatgtaaa gaaaagactg
    17101 gtaattttag tattagagat aaggatactg gatatatact tataacccca tcaggtgtag
    17161 gaagagaaga attaaaagtt gaacatattt gtgtattgga tttggatgga aatgtaatag
    17221 aagttgagaa aggtattaag ccaagtagtg aggtattgat gcatattgaa atatacaaaa
    17281 caagagaaaa tataaatgcc atatcacata ctcattcttt atatgcaaca gcattttcag
    17341 tagcaaagaa accaattata ccaattgtat atgaatcagt taattatggc ggttatgtag
    17401 atattgcttc atacgaaaga ccaggaactt taaaattagc taagagtgtt tcaaaattac
    17461 tgatagatac agatgcatgc ttattagaaa gacatgggtt gacaactgta ggtaatgatt
    17521 tagacgaagc attattaaaa tcaagatatg tggaagaggt agcagaaata tactataggt
    17581 ctttagtatt aaatcaattt aaagaacttg aagtagtaga tgttgatgag tttaaatctt
    17641 ggaaataccc agaagaaata aagttttaat ctaaataatt ttcattgaag tggttaatta
    17701 aaatatatgt attatttata tcaattacta tgttttatta tgttaattac cgtattttgc
    17761 aaatatattt attacatttt ttcaaaaaaa tatagaatcc gaaagccata taaaattgac
    17821 ttgggattct atacttttta tttatgcatt tttctttatc tgattttctg gagaatttat
    17881 atgttcaaaa tattttttag tttcagatac tattataggt gataatgcaa gtaaagctat
    17941 aaggttaggt attgccataa gcccatttac aatatcagca attatccaaa caacttctaa
    18001 ctttaagaaa gcacctaata acaccatagc cacaaataca gttctatacc cattcatgcc
    18061 ttttacacca aacagaaatt caaaacatct ttctccataa taactccatc ccaaaattgt
    18121 agtaaatgca aataatgtta aactaactgt aagtaatatt ggtccaaatg taggaattgc
    18181 tccgttaaaa gctgcttgtg tcataacagc tccatttagg tctgaacacc acactcctga
    18241 aataacaaga gagaatccag taagagtaca gattataata gtatctataa aagtacctgt
    18301 catagatata agaccttgtt ccgctggcca ttcagtttta gcagcagctg cagctattgg
    18361 agcactacct agaccagact catttgaaaa tacaccacga gcgataccat ttctaattgc
    18421 cagtgacata gttgctccta aaaaaccacc agtagcagca gtaggtgtaa atgcactttt
    18481 aattattaac ataaaagttt cagggatttt attccaaaaa gatattaaaa tgattccaca
    18541 gattacaaca tatataactg ccataaaagg tactatttta gtagctacat tagatatact
    18601 tttaagtcct ccaaatacaa ctaaagctac aaatactgct aatacaaccc ctgtaattct
    18661 agggtctatc ccgaaactat tatttattgc atctgttatt gaatttactt gagtaaaagt
    18721 accgattcct aaaagtgcaa ctagtatacc acttatagcg aaaaatattg caagaggtct
    18781 ccatttttct cccataccat ttactatgta atacattggc ccaccagaca cttgaccatt
    18841 tttatcttta gttctgtatt ttattgcaag aacaccttct gaatacttag tagccattcc
    18901 aaagaatgct gctatccaca tccaaaaaag tgcaccaggt ccaccagctt tgatagctgt
    18961 agcaacacca acaatattac cagtaccaac tgtagcagct agagcagtac ataaggctgc
    19021 aaaacttgaa acatcaccac tacctttgtt ttcagcagag aatattaact taagagctct
    19081 tggcagttta gttatttgaa caactccaag cttaacagta agtagtatac cagtaccaac
    19141 taatagtacc agtaggggtg gtccccaaat aaaagcatca actttgttta aaatatcaat
    19201 tagattcata aaaacactcc ctcatttttt ttgctttaca taatcaatag aatttttaat
    19261 gaagtaattg acaggaaata agtataattt attaataatt tctagtgtaa taaaaaaagc
    19321 taaatctaaa aaagattcag ctctggatgt acactaatta gcaaccaaaa taaaagtatt
    19381 agtaaatact gtttttggta gataatccct gtccttttgc ctgagagatt gagcataaaa
    19441 gccttgcccc ttcggcgccc ataatgggtc tctccagagt tccgtaaatt ttacagtcct
    19501 acttttaaaa gtacctgaga gtgttactcc ttcggtgtgc tatatagcaa ctcttctata
    19561 aaatcttaac cggatattga tttgtcatag cttattgtat taataatatt aatataagtt
    19621 tttatgtttt gtcaactaaa aaattaaaaa acaaagaaaa aatataaaaa tgtaagccta
    19681 tttacgaatt ataaacttag aaagatgagt aatatacgtg aaaatgaatt ttgtaataca
    19741 catacaaatg aagtgtaaaa taagttttaa ataaaaaaat aataaaatct taaataatta
    19801 attagatatg attaaattat ggtaatatta tagttacgat attaagaaaa gttctattta
    19861 gttagatttt aagcgtaata tgagattatg tatagagaaa ggatgtattt ttagatggga
    19921 aaaatattag tgctagcaga aaaaccttct gttggaagag atatagcaaa agttttaggg
    19981 tcaaaaaatg aaaaaaatgg ttatatagag gggccaaagt atgtgataac ttgggcacta
    20041 ggtcatttgg ttacacttgc tgaccccgaa agttatggag aaagatataa atcatggagc
    20101 ttagaagacc tgccaatact tcccaagcac cttaagactg ttgtaataaa aaagagtgga
    20161 aagcagttta atacagttaa atcgcaaatg aatagaaatg atatagatga aatagttata
    20221 gcaacagatg caggtcgtga aggagaactt gtagcaaggt ggataataga aaaatcacag
    20281 gtaaaaaaac ctataaaacg tctttggatt tcttcaagta cagacaaagc aataaaagac
    20341 ggatttgcaa aattaagaag tggaaaagaa tatgaaaatt tgtattactc agcaatagca
    20401 cgagcagaag ctgattggat tattggaata aatgcaacca gagctttgac aacaaagtac
    20461 aatgcacagc tttcttgtgg aagagtacaa actcctactc ttgcaatgtt gcttaaaaga
    20521 gaagaagaaa taagaaattt caaacctaaa gagtattatg gattagaatt aattgctaca
    20581 aagggaaatt cagatataaa atttatttgg aatgataaaa acaataattc ttcaactttt
    20641 agtaaagaaa agatagaatc cacattaaaa aaagtaaaag gtgtagacat aaaaatatct
    20701 gaagtaaaca agtcatataa gaaaaaatat tcacctgcac tctatgattt aacagaatta
    20761 caaagagatg caaataaaat gtttggattt tcagcaaaag aaactttatc tataatgcaa
    20821 aaactgtatg aacatcataa agtacttact tatcctagaa ctgattctag atatttaaca
    20881 tcagatattg ttggaacatt aaaagataga ataaaagctg taaatattag tgattattca
    20941 aagatttgta atagattatt aaaaagcaat attatagcta ataaatcgtt tgttgacaat
    21001 tcaaaggtaa cagaccacca tgcaataata ccaacagaac agaaggtatt catgagtgat
    21061 tttagtgata aagagcgaaa aatatataat cttgtagtaa aaagattttt agcagtacta
    21121 tctcctcctt ttgaatatga gcagacaact ataagtgcaa ctgtagaagg agaaatattt
    21181 aaggctaagg gaaacaaagt aataaattta ggttggaaag aaagttataa agatattgaa
    21241 gatgaagatg agtatgaaag tataccaaat gttttagaaa aagatgtact taaggtcaag
    21301 ggattaaaga taacttcagg aaaaacaaat ccaccaccat acttaaatga gggaacatta
    21361 ttgactgcta tggaaaagaa tggtctaggg acagttgcta caagagcaga tataatagaa
    21421 aaattattca attcattttt aatggaaaag aaaaataaag atatatatat aacatcaaaa
    21481 ggaagacaat tattagattt agttccagta gacttaaaga caccagaact tacagcttca
    21541 tgggaaaaga aattattgga tatttcaaag ggaaaactta gtaaaaatac attcataaat
    21601 gacatgaaag attattcaaa agatattgta ctagaagtaa aaaatagtga taataaattt
    21661 aaacatgata acttaactaa aaatagatgt ccagaatgtg gtaaatttat gttagaggta
    21721 aatggtaaaa aaggtaagat gttgatttgt gaagatagag agtgtaatac tagaaaaact
    21781 atatcacaag taaccaattc aagatgtcct aactgccata aaaaattaga acttcgtgga
    21841 gaaggtgagg ggagactctt cacttgtagt tgcggataca gagaaaagtt gtcttctttc
    21901 aacaagagaa agcaagaaga aaaacagaag gcatcaaaaa aagatgttaa tcaatattta
    21961 aaaaatcaaa ataaaaatca agagacattt aataatccat ttgcagaagc attagctaag
    22021 ctaaaaaaca aataatttta aagttattat tatacacaat tataacttta actgtatatt
    22081 agttatatgt actgatataa tatagtgtat agtttaggca tgaaagtagg gctgttaaat
    22141 ggatttaagt ggcatattta aatactattg taaagagtgt gaaaatacat ggaataattc
    22201 gagtgttgaa ttatttgaga atatagaaac gtatagtaaa gattcacaaa aaaagaggga
    22261 aaaagaatta gataaattgc taaatacaat atcagttcat ttagagaggt atccaagtga
    22321 tgctgtattg agaaaaatgt gggtaaaaaa gggcgaggtt ttcttacaaa agacattgga
    22381 aaaagaaaat atttttaagt tagaaaaaat ggatgtagag gatagaaaaa aatttttaga
    22441 tataacaaaa cagtttatta gagatgctag aaaatttgat gatgatttac ctataggtga
    22501 tattatgcaa gctatgagga atgtatggat ttcaaatgca ttacaattat tatttggtaa
    22561 agaagtatat tattcaaaag ctaactttgc atatagtatg ttatatccat atactgataa
    22621 ttatttagac aatacaaata tagataaaaa tgataagatt ttatttaata actggttaga
    22681 aaaaaggctc ctgggagaac acattaaatc taaggattat catgaaagta aagtatctca
    22741 aatgatagat tatattgaaa gtgtataccc tagagaaaag tttacagaag tttatgaatc
    22801 gttattatta atatttaaaa gtcaagtaaa tagtttaaaa caacatggta aggaaaatca
    22861 tttgtgtaaa gaagatttat tatccatttc tattgaaaaa ggaggttcat ccgttttagt
    22921 agatggatat ttaataagtg gattgatgac aaaggaagaa atagagtttt gtataggata
    22981 tggattttta ttacaaatat ctgatgattt acaagatata aaagaagatt taaaatacaa
    23041 ccataaaact attattacag agatgtcaaa agagggtact ttagataaag ttgtaaataa
    23101 actaataaat tttactattg agttaataga tagttttaaa attaataata aaaataaatc
    23161 tgtaatagct atgataaaga atgattgctt aatgttaatt ttattttctg tagtttataa
    23221 tgctgaattt ttttctgtag gatatataaa agaagtagag aaatttattc catatacaat
    23281 agattattca ttagagattg aagaaaaaat aaaagaaaaa tttaaaaata tagatgtttt
    23341 aaataatgaa aatgaatata aagaaatgat tgatattatt tgtgcagagt agaaaaaaag
    23401 ggagtgtctt agaatagact ttttagttta tgaggcactc tttttgtata aaatcaaatg
    23461 tattttcatc attttaacaa ttaaaaagag cttatcccta tttgagaaag ccctttttaa
    23521 tttaaataaa aatgtcaagg ttaaactatt taaatgcagt aataatccca ttatcatgta
    23581 agttttttat gattgtagct gcgataggga atccaaataa tccagctatt ccaaatagtt
    23641 gagctccaac aaacatacac attaatgtta ttaaaggata taggccaatt tggtgtccta
    23701 ctacctttgg ttctaatatt tgtctaacaa cggttataat tatgtatagt attaaaagtc
    23761 ctattgctaa attaatattt ccattaatga gtgagataat catccaaggt gtcaaaatac
    23821 ttcctgtacc caagactggc aaaatatcaa caagtgctat gatgacagat atagtaaatg
    23881 cattttcaac ctttaggata gtaagtccaa taagtagttc tatgaatgtc actgatagta
    23941 gtattgcata ggcttttagt agttttaaaa gagcattaat actgctattt tttatattta
    24001 ttatcataaa ttgacttttt tctggaaatt gttttaagat aaagtttact attttttgat
    24061 agtcaaaagt aaagaaaaaa gaagcaatta aagtaataat aagttttaac ataaatgcag
    24121 gtgttttact tgcaatacta gcaatagcat tgagtgcatt tgttgaaata gaagctacta
    24181 aagacatcat agattgactt atattatccc aaccaagtat taatgctagg ttaacattag
    24241 gtgtactagc atctatcttt gaaaataaag tatttagcgt tggttgtata cttgatttat
    24301 atatttcagg aagtctaaaa aatacatcac ttacacttgc aaatatcttc acaccaaagc
    24361 taactaataa aaatatacac aacccataaa aaattaaaag tattactata gaaataagtg
    24421 tacgatttaa atttgtatta tttgtaataa gtcttataat aggtcttaag atggatgcaa
    24481 tgataaaact gattacaaaa ggcattaata atggcaataa gaattttaat acaatatata
    24541 caatacttgt tattaaaact acatatgtaa atttaattat aaaattcttt tgcttttgaa
    24601 tatccataat ataaaacctc caatgtattt gatattattc taaatatggt cttttttaca
    24661 gaaaatatac tactgtatat tgattataaa ctataaagct tatgtaaagt aaataaatta
    24721 tgtaaaattt aggttaattt tgtataaaga gatactttta tattttacaa ttaataacca
    24781 aatactaaaa aatattttaa ataaagaact taaaattgtg ataaaattaa tttatagata
    24841 ttaaaaaaag tataatatat ggtttttaag aggtgctaag atgaaaaatt ttagagattt
    24901 aggtgggaac aaaactgagg atggcagaac tgtaaagaag ggattatttt ataggtctgc
    24961 aaaattatca aatctaagtg aaaatgatat aaagatttta aaagagttaa atattaaata
    25021 tatatttgat tatagaagtg atgaagaagc tcgtaaacat ccatcaacaa taatatcaaa
    25081 tataaaaaat ataagaatac cagcaatgag agagctagaa gaatcaggag gaagttttgg
    25141 ttctattgaa gatatgattg atggattgtt tgaaaaagat ggtgcattta atatgttaaa
    25201 taatagttac tataatttgc ctataaataa tccgtcatat aaaaaattag tagaacttat
    25261 aagagactat tctaatttac caatattaaa tcattgtaca gctggtaagg atagaactgg
    25321 tgtggggagt gctataattc ttatgatttt aggggtatcg agagagaata taatgaaaga
    25381 ttatctaaaa agtaatgatt ttgcagataa agagatagaa agatttatag aatataagcc
    25441 taaatttaag gacattccaa aagaaaattt gaaatatata tttggtgtaa atgaagagta
    25501 tatgaaaact gcatttagaa gaattgatga agaatacatt agtgtagaag catatttata
    25561 tggagaattt aatttaaata aagaagaaat aagaaaatta agaaatcaat atctagagta
    25621 aaaggtgagt ttatgagttt aaatataaca gcaagacaga taaatataat aaaaatattg
    25681 ttaaattcaa aagatacaat tagtggtatt gccttaagtc aagagatagg atgttcttca
    25741 aaaactatac aaaatgaaat aaaggatatt aataaacaac ttagaaatgg taaaattttg
    25801 tcaataagag gagttggtta taaactagaa ggaagttttg atgaggtaaa tctaaatagc
    25861 aatctctatg atgatgtaga tagagtagaa tatataatca gaaaaatatt aaccttaagt
    25921 aatgaagaaa aaaatactat aaaacttgaa gatttagccg attctatgta tgtaagtgtt
    25981 tcaacagtaa aaaatgattt aaaagaagta aaaagaatgt tggaaaagta ttcaatatca
    26041 gttaaatcta agcataaaca aggtatatgt gttttggaaa gtgaagataa gatattaaat
    26101 tgtatagttg acttaagcaa taagagagat aatcaattga gtttaaatga ttttttaaat
    26161 gatgatataa aggaaagaaa actattaata aaaaaattat tattagacat attaaatgaa
    26221 aatggattag tattaactga tatagaattt aagagtgtgt taaataatat attagttatg
    26281 ttaagcagac ataagtataa tgaaaaagaa tttatagaga attatataga aaattatagg
    26341 agcaaaagaa gctcaatttt agaaaatgaa caaaacaaag aaattataat aaattcaata
    26401 aaaacatttt gtaaaaattt aaaattagct acatcaatag atataagtaa tgataaggta
    26461 tttgaagagt ttttgtataa gcatattagt agtttatgta aaaaaataaa gttaggtata
    26521 aatcaatcac ctataatggc tcaagatata aaaataaaat atccatttgc ttttgaacta
    26581 gctaatatag ctaaaaagac aatagaaaaa gatttgaaga tagaaattaa tgaagatgaa
    26641 gttgcaaaca tagctctaca tataggagga gcagttgaaa gagcatctta taataataaa
    26701 gataaggttt tcaaaactat aatagtttgt acttcaggca taggcacatc aatgttaata
    26761 aaagctaaat tagagaatat atttaaggaa aaattagaaa tactaaagat tattccttca
    26821 tacttagttg aatatataaa agttatagat gttgattttg taatatctac tgttcctatt
    26881 gaagtaggag atgtgccagt aataaatata tctccaattt taagtgagaa agaaattaga
    26941 ttaattgaaa aatatataga aacaggaaat atatacctag atttagatat aaaaagcctg
    27001 tttgatagtg aattgttttt tactgatttg gtgtttaaaa ggaaagaaga agttatagat
    27061 tttatggcaa gtaaacttgt acaaaagggc tatatagatg aggatatgaa aaaatcttac
    27121 tttgagagag aaaaaatagc aacaacagaa atagggaata tggtaactat accacatgga
    27181 gctattggaa aaatttttga taataaaata gcaattggta tattaaagaa gcctatttct
    27241 tgggaaatat cagatgttcg tttaataata atgttagccc ttgataaaga taaaatatta
    27301 gattatgaag taatattttc aaaaatatat aaaagagtag attctatagc aaaagttata
    27361 agtatatgtg aaaatagtag ttttgaaaaa tttgtaaatt tattcaatta gatgggtagt
    27421 attgtatata cacctgtaaa tttggataat ataaaactaa ataagataaa aacgataaat
    27481 ttctctttta attaagagaa attttttgac tttcaaggat attaatataa agttaagata
    27541 taaacataaa gcaaataaat aaaaaagagg tggcaatatg atttatacag tgacattaaa
    27601 tccatcaata gattatatag ttaagctaga tgagctaaag acaggaagta caaatcgtgt
    27661 aaaagaagaa tacgtttatc caggaggaaa aggtataaat gtatctcgca tatttaagga
    27721 acttggaaat gataatacat ctttaggttt tataagtggt tttactggag aatatataat
    27781 aagaacattg gaagaaaaag aattgaagac agattttata aagataaaaa gtggtttttc
    27841 aagaataaat gtaaaaataa aggcgttaaa ggaaactgag attaatggac aaggtccaaa
    27901 tatagatgat gaagacattg atactttata taaaaaactt gataagctaa atcaagatga
    27961 tattttaata ttggcaggaa gtataccatc tactttagat gaaaaactat acgaaaatat
    28021 aatggctaga ttagaaaaaa agaatataaa agttgtagta gatgcaacta aaaatcttct
    28081 tcttaatgtt ttaaaatata aaccttttct aattaagcct aataacgacg aattagaaga
    28141 actatttggt gttaaattaa attcaattga agacatggta aaatatgcta gaaggttaaa
    28201 ggaaatggga gctataaatg tcttggtatc aatgggaaaa gatggagcat tgttgattac
    28261 agaggaagat gaagtgctta taagtgatgt tccaaaagga aaagtaaaaa attctgtagg
    28321 agcaggtgat tcaatggtag caggatttat atctgggtat ttgaatactg ggaaatatga
    28381 ttatgctctt aaattgggag cagcttcagg tagtgcaact gctttttcat atgacttagc
    28441 caaaagagaa tacattgata aattggtaaa tgagatatct gtaaagcagt tttaaaatat
    28501 taaaaaatat atagaaaggt aattaatatt tatgaaaatt atagatttat tagatgaaaa
    28561 gtcaattaag ttaaatttaa attcaaaaac caaatcagaa gcaatagaag aattagtaga
    28621 tttggtcgca aatagtggaa atttaaatga taaagaaaac tacaaaaaag caattttggc
    28681 tcgtgaagaa atgagtacaa caggaattgg agaaggtgta gctatacctc atgctaaaaa
    28741 ttcttcagtt actaaagctt gtatagcagc agcagtgtca aaagaaggta tagattatga
    28801 atcgtttgac ggaagtttat caaatctatt ttttatgata gctgcaccag acggagctaa
    28861 caatactcat cttgaagtat tatcaagatt atctacaata cttatggatg aagactttag
    28921 aaataaactt ataaattcaa gctctgaaaa agaatttaag gaaataatag ataaaaagga
    28981 aagagaaaaa ttcagtgaag aatatcaaga tgagaaagtt actgaaaaaa atataataga
    29041 tactaaagag aatgatgcta ataagtatcc taaggtttta gcagtaacag catgtccaac
    29101 tggaatagca catacattta tggcagctga aagtttaaat aaaatggcag aaaacaaagg
    29161 tgtatcaata aaagtagaaa ctaatggttc agcaggtgtt aaaaataagc ttacaaaaga
    29221 tgaaatagaa aatgcaacat gtataatagt ggcggcagat aaaaatgttg agatggctag
    29281 atttaatggt aaaaaagtca taaaaactaa ggtggctgat ggtatacaca aagcggaaga
    29341 acttatagac aaggctgtaa atggagatgc tccaatttac catggaggag atggaagtca
    29401 caacgaatca aatgaagaaa gtgaaagtgg atttagaaaa gtatacaaac acttaatgaa
    29461 tggtgtatct aatatgttac catttgttat tggtggaggg atacttatag cgatagcctt
    29521 tttacttgat gattatacta ttaacccttc aaactttggt tcaaatacac ctatagcagc
    29581 attttttaaa ggaataggag ataaagcttt tggatttatg ttacctgtac ttgctggata
    29641 tatagcatac tctatatctg atagaccagc gtttgttgta ggatttgtag gaggtgctct
    29701 agcaggtgat ggtggttcag gttttttagg tgcactactt gcaggtttta tagcaggtta
    29761 tttagtagaa gggcttaaga aaatatttag tgtactacca gcttcattgg aaggaataaa
    29821 accagtattg ttataccctt tacttggaac acttttgatg ggaattataa tgacattttt
    29881 agtaatacct ccagttacag caattaataa tgcaatggtt ggatttttaa atggtttagg
    29941 tggaacttca aaaatagttt taggattagt attaggtgga atgatggctg tggatatggg
    30001 tggacctgta aataaagcag catatgtatt tggagttgca tcacttgaat cagggcagtt
    30061 tgaaataatg gcagcagtaa tggctggtgg tatggttccg ccacttgcta tagctcttgc
    30121 aactacattt tttaagaata gatttactaa agaagaaaga gattctggta aagttaatta
    30181 tgtaatggga ttatcatttg ttactgaagg agctatacct tttgcagctg gagacccact
    30241 tcatgttata ccagcttgtg ttggaggttc agcagtggca ggagcactta gtatgttgtt
    30301 caacgcagct cttcgtgcac ctcatggagg agtatttgtt ataccagtgg ttacccatcc
    30361 atttgcatat atattagcta tagctgtagg tgctttggtt ggaatgatgc ttttagcatt
    30421 acttaaaaaa ccattaaatc aagaagtata gcaattaaag gatattcaag aacttaaaat
    30481 atgatgtttt ataaaaatta atatattgct gaattatttt aaaataagga ttttatgagg
    30541 ctgttatata acgatttgta attgttatct tgacagtctt ttatttttat ataagtaaaa
    30601 taagtgt
//
LOCUS       DAYASV010000033        30323 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYASV010000033 DAYASV010000000
VERSION     DAYASV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30323
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.033"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            144..686
                     /locus_tag="ACGI68_001522"
     CDS             144..686
                     /locus_tag="ACGI68_001522"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0137966.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            1014..2507
                     /locus_tag="ACGI68_001523"
     CDS             1014..2507
                     /locus_tag="ACGI68_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0137967.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNILVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKVLFEEYSKG
                     ILKKVSKRKKVDKNSYKSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
     gene            2527..2814
                     /locus_tag="ACGI68_001524"
     CDS             2527..2814
                     /locus_tag="ACGI68_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137968.1"
                     /translation="MLCYRNEVAKNTIEVVKDLKAEDMKRKVSPIDLERILVTGGVIK
                     HEDSIWLLDFWGNKDIAGILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            2970..3734
                     /locus_tag="ACGI68_001525"
     CDS             2970..3734
                     /locus_tag="ACGI68_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0137969.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIINFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            3755..3889
                     /locus_tag="ACGI68_001526"
     CDS             3755..3889
                     /locus_tag="ACGI68_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137970.1"
                     /translation="MKTVKWIAKLTKVSVHILHYYDEIGLLKQNILIVKLLHLYMQYV
                     "
     gene            complement(4019..7198)
                     /locus_tag="ACGI68_001527"
     CDS             complement(4019..7198)
                     /locus_tag="ACGI68_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFH0137971.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGINKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLDKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            7479..9656
                     /locus_tag="ACGI68_001528"
     CDS             7479..9656
                     /locus_tag="ACGI68_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFH0137972.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYKQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASVRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEANCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            9670..10092
                     /locus_tag="ACGI68_001529"
     CDS             9670..10092
                     /locus_tag="ACGI68_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0137973.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            10266..10841
                     /locus_tag="ACGI68_001530"
     CDS             10266..10841
                     /locus_tag="ACGI68_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0137974.1"
                     /translation="MVKVLAFMGSPRKKGNVDTILDEIIKGVNDNNVEVKKYYLNDMN
                     IKGCQGCLYCRKVPTCAFKDDMIDIYEDIKSAEYIIIGSPVYICQVSAQTKLLLDRLY
                     PLTEINKSKHIPRFGQKKLIMVYTQAAPISFLFKKYFKYTAKHLKGMGLNHYKTIVVT
                     KAFERNSTKKNFKVLKKAYKLGMKIAIDING"
     gene            complement(10966..11682)
                     /locus_tag="ACGI68_001531"
     CDS             complement(10966..11682)
                     /locus_tag="ACGI68_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0137975.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFQNKLDI"
     gene            11847..12572
                     /locus_tag="ACGI68_001532"
     CDS             11847..12572
                     /locus_tag="ACGI68_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0137976.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSTSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASFLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            12632..14224
                     /gene="hcp"
                     /locus_tag="ACGI68_001533"
                     /gene_synonym="priS"
     CDS             12632..14224
                     /gene="hcp"
                     /locus_tag="ACGI68_001533"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFH0137977.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            14596..15501
                     /locus_tag="ACGI68_001534"
     CDS             14596..15501
                     /locus_tag="ACGI68_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0137978.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            15625..16578
                     /gene="msrA"
                     /locus_tag="ACGI68_001535"
     CDS             15625..16578
                     /gene="msrA"
                     /locus_tag="ACGI68_001535"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFH0137979.1"
                     /translation="MIKLATFSGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            16731..17861
                     /locus_tag="ACGI68_001536"
     CDS             16731..17861
                     /locus_tag="ACGI68_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0137980.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(17978..18946)
                     /locus_tag="ACGI68_001537"
     CDS             complement(17978..18946)
                     /locus_tag="ACGI68_001537"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFH0137981.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            19281..19469
                     /locus_tag="ACGI68_001538"
     CDS             19281..19469
                     /locus_tag="ACGI68_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137982.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            19729..20928
                     /locus_tag="ACGI68_001539"
     CDS             19729..20928
                     /locus_tag="ACGI68_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HFH0137983.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIENDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAINLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            20980..21483
                     /locus_tag="ACGI68_001540"
     CDS             20980..21483
                     /locus_tag="ACGI68_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0137984.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVVHS"
     gene            21590..21907
                     /locus_tag="ACGI68_001541"
     CDS             21590..21907
                     /locus_tag="ACGI68_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137985.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            22065..23024
                     /locus_tag="ACGI68_001542"
     CDS             22065..23024
                     /locus_tag="ACGI68_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0137986.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            23139..23924
                     /locus_tag="ACGI68_001543"
     CDS             23139..23924
                     /locus_tag="ACGI68_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137987.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            24216..25535
                     /locus_tag="ACGI68_001544"
     CDS             24216..25535
                     /locus_tag="ACGI68_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0137988.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(25610..26038)
                     /locus_tag="ACGI68_001545"
     CDS             complement(25610..26038)
                     /locus_tag="ACGI68_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137989.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(26129..27505)
                     /gene="hydG"
                     /locus_tag="ACGI68_001546"
     CDS             complement(26129..27505)
                     /gene="hydG"
                     /locus_tag="ACGI68_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HFH0137990.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(27506..28591)
                     /gene="hydE"
                     /locus_tag="ACGI68_001547"
     CDS             complement(27506..28591)
                     /gene="hydE"
                     /locus_tag="ACGI68_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HFH0137991.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(28627..29835)
                     /gene="hydF"
                     /locus_tag="ACGI68_001548"
     CDS             complement(28627..29835)
                     /gene="hydF"
                     /locus_tag="ACGI68_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HFH0137992.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTQKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGVLIAYVKGI
                     LERSLELFNY"
     gene            complement(29850..30101)
                     /locus_tag="ACGI68_001549"
     CDS             complement(29850..30101)
                     /locus_tag="ACGI68_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HFH0137993.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
BASE COUNT        11538 a         3480 c         4822 g        10483 t
ORIGIN      
        1 gataaaataa gacaaatatg ttaaaaaaat gataatcacg gagaactttt accttcttca
       61 attaatatat tattattagt aataacttta aaattatatt actattatat aaaaattggc
      121 ttaaaagaag gtgaaaaaag taaatgaaaa tatgcataac agtaggtcat agtatactaa
      181 aaggtggtaa aagtacaggt gtaaatggta tagttgatga atacaattat aataaaaaat
      241 tagctcctat gttagctgag atgcttataa gtcaaggtaa tacagtagat gtaataattt
      301 gtccagaaag gcactttatg tcagaaagag aagaattttt ctatagagtt ccaaaagtaa
      361 acagtggtaa atacgattta ttagtagagc tacatttaaa taaagctgat ggaacacaat
      421 gtggtacaga agtgctttac tatggcactg aaggattaga atatgctaaa agagtatcta
      481 atagacttgg agaattattt gaaaatagag gagctaaaaa aagagaaaat ttatatatat
      541 tgagaaatac aaatccagtt gcaatacaga ttgaaagttt tttctgtgat aatgtgaatg
      601 attgtaacaa agcaaatgaa tcaggttatg attatatagc acgattgatt acagaaggta
      661 tattgaataa agatataaat atgtgagatt atggtgattt aggtattgat taataccttt
      721 taatattaga tataaataaa aatacaatgt attagtataa tttaaacttt caaaagatag
      781 attagtttga ttattgacaa gagatacctc cttgaaaata ttgatttatc agtatattta
      841 aggaggtatt taaatataaa aatttaatta atttgctact aaactatagg tttatagagt
      901 atatactttt atcttcagtc taataaataa tataaaaata gtagaacctt gtattataat
      961 aatataatgt agaaaaatat atagataaga taaatataat aaggtggtaa tatatgaaat
     1021 atacatttga aaaatttaag ttaaatgaaa agatacttaa gtcattgaag agtttaggat
     1081 ataatatacc ttctagagta caaagagaag ttattccaaa attattaaaa ggtcaaaact
     1141 tagtagttag gtcaaaaaca ggaagtggaa agacagcaag ttttgcaata cctttatgcg
     1201 agaatataaa tgtagattac aataatattc aagctcttat agtggttcca acaagagaat
     1261 tagccttgca agtaaaagat gagatatcag atatagggag actaaagaag gtgagatgta
     1321 gtgctatatt tggcaaacaa tctataaaag accaaatagc tgaattgaaa caaagggttc
     1381 atattgttgt agctactcca ggcagaatac ttgaccatat aaatagaggt tctattaagt
     1441 tggaaaatgt aaagtattta gtaatagatg aggcagataa aatgtttaat aaaggttttg
     1501 ttgaacaaat ggaaaaaata ttattaaatt tacctaagga gaaaatagta agtctttttt
     1561 cagctacaat agatgaagag ataaaatata tatgtgaaaa atatatgcta gattacagtg
     1621 ttataaatat agaagaaaat gagagcgata ctaatcaaaa aacaagacaa atagatgaca
     1681 aaataatcaa ggcaaatgga agagagaagt acatcctttt aaaagaatta atatacagcg
     1741 aaaatccaaa gagtgtgata atattttgta ataccaaaga gaaagtatct aaattatata
     1801 acaagatgag taaagagggt tttttaataa gagagttaca tgcagattta tcacaagaaa
     1861 gaagaatttt tgtaattaag gattttaaaa accaaaaatt taatatatta gttagtagtg
     1921 atgtagcgtc cagaggaata catattgatg atatttcttt agtaataaat tatgatgtgc
     1981 cacaagataa agaaaactat atacatagaa taggtagaac tgggagaaaa ggaaatagtg
     2041 gaaaggcaat tactatagta actgaaaagg atgaaaaata tatagagaat atagaaacgt
     2101 atataggata taaaataaat gaattaaaag atatagaaaa aagtagaatt acacatggca
     2161 aagttttgtt tgaagaatat tcaaaaggaa tcctaaaaaa agtatcaaag agaaaaaaag
     2221 ttgataaaaa ttcttacaaa agtaaagata taaaactaaa tatagaaagt gaagttgtta
     2281 aattatattt aaatgctggt aagaagaaga aaataagagt tttagatata gttggtgcat
     2341 ttagtaatat aaaaggtata actaatgatg atataggtgt aattgaagtt caagatttat
     2401 gttcatatgt agatatatta aattataagg gagatttgat attaaaaaaa tataaggaga
     2461 tacctataaa gaaaaaaatg gttaaagtga aaagagatat acgataattt agtcaatatt
     2521 gatgaattgc tttgttatcg aaatgaagtt gcaaaaaaca caatagaagt tgttaaggat
     2581 ttgaaagctg aagatatgaa acgaaaggtc tctccaattg acttagaaag gattttggtg
     2641 actggaggag taataaagca tgaagattct atttggttgt tggacttttg ggggaataaa
     2701 gatattgctg gaattttgct tatgcctcct actcgtcatg ttatacttca tttgaatgat
     2761 tgttgtaaat ggaaggaata ttttcgtaca aaaaagaaat tttacagaag ttaaattata
     2821 ataaattaga caaacaatag ataaattagc aaggaatgtt gattttatct acattctaag
     2881 ttatactatt tttaagtggc taagggaata gtcattttaa atataagtaa taaatatata
     2941 cctataggga tattaaaggg gggaaatata tggcatattt taactataac gacaaacagt
     3001 gctactataa tgaaattggg gatggtacac cattactttt tttacatgga aatacagcct
     3061 cttccaagat gtttaatgat ataatcaatt tttataaaga tgaatataaa gtaatattaa
     3121 ttgattttgt aggtcatgga aaatctcaaa tggtcgataa attttctgct gacttatggt
     3181 ttgatgaggc tatgcaagtg atatgttttt tagaagcaat gaactataaa aaagtaaaca
     3241 taattggtag tagtggagga gctcttgttg cattaaatgt tgcacttgag agacctgatt
     3301 tagtgaataa ggtaattgca gatagttttg aaggagaagt tcctctagag tcatttgtac
     3361 aaaatgtgaa aatagagcgt gaagcatcta agcaagatga tggtgcaaaa gctttttata
     3421 tttataatca aggagaaaac tgggaaaaag ttgtagataa tgatacagaa gctatttttg
     3481 aacattataa aactatagga aagttttttc ataagcctct agaaactatg caaccagagg
     3541 tgcttttaac aggaagtaga gaagatgagt ttgtatctct aatatctagt gatttttttg
     3601 aaaatacttt ttcattactt cttgaaaaga ttaaaaatgg taaaatgtat ttgtttgata
     3661 aaggtggtca tcctgcaatt ttatcgaatg gattagattt ttcaaatgta gcaaagaaat
     3721 ttttagagga ataatttaac caaaatttac aataatgaaa actgtaaaat ggattgcaaa
     3781 acttacaaaa gtgagtgtac atatacttca ttattatgat gaaataggat tgttaaaaca
     3841 aaatattcta atagtcaagt tattacattt gtatatgcaa tatgtataga aagagatatc
     3901 attagattgt aaagttggga ttaaataaca atattaaatt ttatttattc agatatttta
     3961 gcttaactat ataaatgtta gaacaaattc atatagttaa gctgtaggat taaataattt
     4021 agttaaataa gtttattatc tcctcatcag atagactttt taatacacct tcattagaaa
     4081 gttctccatt tataaattga tttataagct cttttttact ttcttgtaat tttattattt
     4141 tttcttctat agtcccttta gcaatcaatt ttataacttg tactgaattt ttttgaccaa
     4201 atctatgagc tctatcacta gcttgatttt ctactgatgg attccaccat gggtcaaagt
     4261 gaattaccat gtcagcagat gtaagattta aaccagttcc gcctgctttt aaagatatta
     4321 aaaaaacttt tttatccata ctattattga actcatctac aagttcaagt ctttctttgg
     4381 cattagtttt accatctata taatggtatt taattttata cttatcaagt tccttactta
     4441 tgttcttaag cacagaagta aattgtgaaa acaataatat tttgtggttt tcgtttatac
     4501 tatccctaag tatttctaaa catgtttcaa tttttgaact ttttttattg taatctttta
     4561 ctactatact tgggtctaaa caaagttgtc ttagtttagt taaataagag aatataacta
     4621 ttttatcttt ctttagattt ttatctttca ttttatcttg tatatctttt gaatatacac
     4681 tatatatctt tttctgctct ttattcaatt caacaaaaaa gtttttttca atcttatctg
     4741 gaagttcttt cataacttgt tttttacttc ttctaagaat aaaaggtttt atcaattttt
     4801 tcaggttttt aacattatct tctttattta taaacaattc ctgaaacttt gctttactat
     4861 atagatagcc aggcattata aaatcaaata tagaccataa ttctagtaga ttattttcca
     4921 taggtgtacc agtgagtgca aatttatttt ctgcatttat actttttaca gactcagtta
     4981 ctaaagccac tggatttttt atattttgag cttcgtctaa tatacaataa tcaaacttta
     5041 tttcactata tttgtctaaa tcatttctaa gagtaccata tgttgtaagt attacatcaa
     5101 aattttctaa ttccataaaa catttttctc tatctctttt atttccatgt atcaataaaa
     5161 cttttatatc tggagcaaac ttttcaaatt catttttcca attataaatg agtgatgtcg
     5221 gtgtaacaat aagacttttt ttattagata atgataataa aaatgcaata gtctgtattg
     5281 ttttaccaag acccatttca tcagctaata taccaccaaa tttataataa tctaaaactt
     5341 taaaccaatt gaggccattt atttgatagt cacgaaggtt tgcatttaaa tttgcgggaa
     5401 tttctaaatt tatgctatca aaatttctaa atttttcaca tatactgtct agttcttttt
     5461 taccatctat aaactttaaa tttttactac gtattacatc atttatatat agagctttat
     5521 ttttatgaat tttattattt tttgatttat ccattatatc tagattttca actaattcaa
     5581 aaaaatcttt tgtttctctt tctgataaat ctataaagtt tccatcttta agtttataaa
     5641 aagtacgttt ttcttgaaaa gctttcaaaa tatccttgta ctcttttgga tttatatcag
     5701 atatattaaa attaaattct aggtaattct ggttagtctg attgattgaa gcttgaattg
     5761 atgaagcgtt atatattttt ttcaatttaa acttctcaga ataatatata tctccaaatt
     5821 cctttagaga ttctaattgt aaatctaaaa atgtaaatag gtcatggtca gttcctttaa
     5881 acacataatg attattttcc tttgtaaatt gtgaagaagt aagtttattt tcaatagtag
     5941 cttccttttg tatgttgggg agcacaaatt ttttatcttc atcatcatat atatacttta
     6001 catcacaaca tatgttgcca tcaatcatat caaagtagaa ctcagattta aaatcttttg
     6061 ttatattatt ttttatgtta tcatctaaat gaacttcttg agatatagat tttaatctag
     6121 gaactagcaa tcctaaaaca tccttagaat cctctttatc aaattttaac tctttagtct
     6181 ttgataatat ttgatatatt tttgaataat catgagcttt tttatgtgat aataaataga
     6241 tattgccatc atataaaagt acatcatttt ttttagtaag tggaacaggt agtgaatctt
     6301 tagaatataa gactatttta tcttctatat ttttaaaatc aaaccttaca ggtagagtat
     6361 catttaatat ctttggatga tattcctctt tagaaaaaat aagggtaaaa tccttgtact
     6421 taatagcatt taaaaattgt tttaaagagc ttgcatttat ctccataaat ttttttgaat
     6481 gtgatgaaga aaagttgtat gaaagatttg tcccatattc ttctataata tctactaact
     6541 tctcatcttc atcagcaaaa taatgagtgt tggggttgta agtaaatttc ttcccaaaat
     6601 ctaattcata tccattaagt tttgaatatg caaattcagt aatgtttttt aaaatgtaca
     6661 ttttattaat tcctatttta aattcagcaa ttgctttatt tgaatccaca tttatcaata
     6721 catctaaatt aaccctttct cttttagtat tgtctaacaa agaaagtaat tgcttgctta
     6781 aatctaagct ggttttagaa tttgtattag aaagcttatc cttatctgca cctaatatat
     6841 ctattagaat aggaggaaca tcttttttat tttttatttg ttttaagctt tttatcacaa
     6901 tagcaactat atgcttacat ataaaaatac ttttgccctt tgattgacag tcagcgcaac
     6961 tacaatcatt ttcaattatc ttaaagtgct ttatatctat agttattgaa ttaaaataaa
     7021 agctataatt atcttcagaa ttgactccac cacgaaatgt caacagatta ttttgtaggt
     7081 catcaatatc tatgtctata taatcaacta attgattagt ataatatttt acacctcttg
     7141 accatcttga tggttctgta tttcttctta ttagtttata tatcatatct attttcatat
     7201 tattcacctt acctcactgt atatattata atctaaatgc attcaattta cactctaaat
     7261 ttaagactaa aattaaggca gatatgaata gcttttatct atcacgatat atgtgtaaat
     7321 aacaatttac tattagattt ttattgaatt atcttataaa ctatgaatga aaaataatta
     7381 tataaataga ttattaataa atataaaatc ttattattaa aaatattctt agaaattaca
     7441 ctgtataaaa tataaagtat gatatttgaa taaaaagaat gtataataag tataataatt
     7501 tgtatttatt attgggaaag gaaaatgtta atatggatag ttataaacaa cttaaagcta
     7561 aaataccctg taaggaaaca ggtatagaaa taaagaggac tttatgtgat atttgttcgc
     7621 caggagtaca ttgtggaatt gatgcatatg ttaaagatgg taaagtaata aaaatagaag
     7681 gtacaaaaga acatccaatg aataaagggt tgttatgtac caaaggtctt tgcaatagag
     7741 aatatatcta tagagaagat agaataaaaa cacctctaaa aagaattgga aaaagaggtg
     7801 aaggaaaatt taaaccaata tcatgggatg aagcttatag agaaatagca gaaaaattaa
     7861 atagagtaaa atctgagtat ggagcaaagc atgtagcttt ttttacgggt tatggaaagt
     7921 ggtatagacc ttttttacaa aggtttgcat cttcatttgg ttcacctaat tatggtacag
     7981 aatctagttc atgttcaaca tcagcacaga tggctggaaa tgttgctact ggaagaaact
     8041 ttacaatgca agatatggag aatgcaaaaa cttatttagg gtggggattt aatccatact
     8101 actctaaatt tttaaatagt gtgaatctaa taaaattaaa agaaaaaggt ctaaaattta
     8161 taattgtaga ccctagaata acaccgacaa ctcaaaaact agcagattta catttaagaa
     8221 ttaaacctgg aactgatgga gctttagctc ttggaatggc gaatctattt atacaaaagg
     8281 atttaataga tagagaatac atagaaaagc atgtacatgg atttaatgaa tataaagaat
     8341 atgtaaaatt atttgattta gatactgtaa gtagaattac aggagtttct aaagataata
     8401 tagtaaaagc tgttgagatg ttgtcacaag gtccattttg tataaatgaa agtgcagctc
     8461 caatagttca tcatcaaaat ggatttcaaa attatagagc aatgatgtct ttatccgcta
     8521 taacaggtaa ttatgataga ataggtggaa acataccaaa acaagattac tttgctggta
     8581 agataggtgg attccaaaca ctagaaaaag agtttgtaaa agaagttcta cctaaaaata
     8641 taaatgaaag aattggtgga gaaagatttc ctctatggaa tgaaatggtg actcaatttc
     8701 aagctatgga tttacctagg caaatagaag aaaagacacc atatgaaata agagctttgt
     8761 tttgtcatgg gataaatgct agaatgtttc ctaattcagg gagacttttt aaagcgttga
     8821 atagtgtaga tttttttgtg aatatagatt tatttatgac agatacatca aaatatgcag
     8881 atatactttt acctgcatgt acatcatttg agagagaaga atgtaagagt tatccaggag
     8941 ggtatattac atataccaaa aaagttattg ataaattata cgattcaaaa tcagatgtag
     9001 aaatactttc agatttagca aatgctatga atatagatga tgatttatta aaagcaggtt
     9061 atgaagcaag tgtaagacat atgtttaaaa atacatgtgt agatgtagat aaacttaaag
     9121 aaagtgattt accattaaaa tgtaaagatt ttaagccata tatagtaggt tcatatacaa
     9181 gagaagggta tgatactagc acttctaggt ttgaattaaa atctacaatt attgaaaaat
     9241 ataaagattt tgggctggat gcactaccaa catatagagg atttaatgat aatatagatg
     9301 atgaatatac tcatttattg acatcaggag caagaatttc aaatgcacta cattcaagac
     9361 ttcatgacat caaactttca agaatattaa aaaatgaagc taattgtgat atttctagct
     9421 tagatgcaaa agaattgggt gttatcagtg gagattatat aaaaataact actaagatag
     9481 gaagtttgga tgtaaaagtc aatgttactg atagagtctt atataagaat atacatatgt
     9541 atcatggata tagagaagct gatataaata gtattatttg tgataatttt gacccttata
     9601 gtggattccc tgcgtataga agttgtaagt gtaagattga aaaaatgaat agataaatgg
     9661 agagatataa tgaaaagttt atttaaattt aataaagaat tatgtacagc atgtggagca
     9721 tgtttgcttg catgtataga ccaaaatgat aataatatag acaatggtga actttttaga
     9781 catgtattta caatagagga aggtgaaaat atagaatatt tttcattagc atgtatacat
     9841 tgtaatgagc caaaatgttt aggtgtatgt cctaaaaatt gttttaaaaa agaagaagga
     9901 ttcattgtat tagacaatca aaactgtata gggtgtaaac tttgtgaaaa agcttgtgaa
     9961 tatggagcct taatttatgg aaatgatggt aaggcaaata agtgtaatgg ttgtattgag
    10021 agatataagt gtggtatgga ttcaccttgt gtaatggtat gccctacagg agcattaaaa
    10081 tttaataaat aattgtatta aattgctata ctacttttaa ataattgtgt taagaattat
    10141 tttttgatat acttttgtaa tataaaatgt aaataaaaca aatacaaagt atataaaata
    10201 tatttttaga ataaagtgtg ctttagatta aatttagaaa aataaattag acaggagaac
    10261 ttaatatggt aaaagtatta gcctttatgg gcagtccaag aaaaaaaggg aatgtagaca
    10321 caatacttga tgagataata aaaggtgtaa atgataataa tgttgaagtt aaaaaatatt
    10381 atctaaatga tatgaatatt aaaggatgtc aaggctgtct atactgtaga aaagttccta
    10441 cttgtgcatt taaagacgat atgattgaca tatatgaaga tataaaaagt gctgaatata
    10501 taataattgg ttcgccagta tatatatgtc aggtatcagc tcaaacaaag ctacttttag
    10561 atagattgta tcctttaaca gagataaata agtctaaaca tatacctagg tttggtcaaa
    10621 aaaagttaat catggtctat actcaagcag ctcctatttc gtttttattt aaaaaatatt
    10681 ttaagtatac tgcaaaacat ttaaagggca tgggattaaa tcactacaaa actatagttg
    10741 taaccaaagc atttgaaaga aattccacta aaaaaaattt taaggtattg aaaaaagcct
    10801 ataaacttgg aatgaaaata gccattgata tcaatggata gataaaagtt taatttatag
    10861 tatagctaaa ggagcattaa ttattaatac tccttttttg tataaaggtt tctagaaaat
    10921 aactaataaa aaacaatatt atttgaatac agatattata caagcctaga tatctaactt
    10981 gttttgaaaa taaaagattt caaatttatt tttgttaaaa gataacaagc cttcatctcg
    11041 catcttgcca agctctcttg acatagaact tctgtcaaca cataaaaaat cagccatttc
    11101 atttcttgaa aagggaattg taaactggtg gctacctgtt tgttctatct gttttgaaaa
    11161 atacataagc aatttctcgc gagttgtttt acgagaaagt aattctattt tattgtgaag
    11221 taaaatgttt ttttcagcta ttaattgtag catattttca acaagcctat tatgaaatac
    11281 acatgcagat gaacaaatac cagttacact tttaaacttt ataaataaaa cttcgcaaga
    11341 agatgttgtg gttactgtaa cattactttt atgtagtcta gtacatgcga atacctctcc
    11401 aaataaatct ccaggcaaga gttcagctac aatattccta ttcccttcga tatcttcctt
    11461 tattatatga gcacttcctt taagcataat tccaatagaa gtaacttcac ttccaacaga
    11521 gaatattata ctatcttttg ggtaagtctt atctacaaca cctaaacatt ccaacatttc
    11581 ttcaagttca tcacttttaa ttccttcaaa tagaggaatt tttttaagta agtctatatg
    11641 cttttctaaa aattttttca aataatcact tccttgttgc atatgcaacc gataatttgt
    11701 tttcattgta ctatactaag tttataaaag caaggggata taaaaatcct actacaagaa
    11761 atttgaaata aagaaatata ggtattaaaa ataaatatat aagaaatcaa tactttaaat
    11821 taaaaaaatt ttatgaggtg atataaatga tgagaaaaat aattaaaatt gatgaggaaa
    11881 agtgtaatgg ttgtggacta tgtgttgaag cttgtcatga agaagctata ggaatggtaa
    11941 atggaaaagc aaaactttta agagatgact attgtgatgg gctgggagat tgtctgccaa
    12001 cttgtccaac aaatgcgatt tcatttgagt atagagaagc agcagaatat gatgaagctg
    12061 cagtaaaagc aaatatggaa gcaaagaaag cacaaaagaa aactttagca tgtggatgtc
    12121 caggttctca gtctaagtca ataaatagag aggtttcaaa tagtacgtct ataagcaatg
    12181 atatagtaga gataaaaggt tctcaactta atcaatggcc agtacaaata aaattagtac
    12241 caactaatgc atcatattta aagaatgcaa gttttttaat agcagcagac tgtacagcgt
    12301 atgcatatgg aaattttcat aataaattta tgaaaaataa ggttacatta atcggatgcc
    12361 ctaaacttga tgaagttgac tatgctgaaa aacttacaga gattttaaaa gaaaatgata
    12421 ttaaaaatat agtagtaaca cgtatggaag ttccttgctg tggtggtata gtaaatgctg
    12481 ttaagacagc tctacaaaat agtggaaaaa tgataccatg gcaaatagtt actatatctg
    12541 tagatggaaa aattgtagat ggagaaattt aattaagtga attatagaaa aatataataa
    12601 attttaataa ataaaaaggg aggaataaaa aatggaaaaa atgttttgtt ttcaatgtga
    12661 acaaacagca ggaggtaaag gttgtactat aaatggtgtc tgtggtaaaa aggggggtac
    12721 agctaactta caagacgaac ttacaggagt gttgattggt ctttcaagag caactattgg
    12781 aaataagaat agaccaacaa gtgaaacaga taaaattatg atagaggggc tattcactac
    12841 tataacgaat gtaagttttg atgatgaagc tataaaaaga caaattgaaa aaactgaggt
    12901 agaaaaaagt aaacttgtac ctagatgttc agattgttca acaacttgta atagaaatga
    12961 taactatgat atgaaggatt tatggaatga taatgaggac atacgttcat taaaatcatt
    13021 aatcttattt ggattaagag gtatggctgc ctatgcatat catgcaatgg tacttggtta
    13081 tacagacaaa gacgtaaatg aatttttcta tgaaggatta atagcagttg gaggagactt
    13141 gtctattgac gaacttttag gtctagttat gaaaactgga gaaataaact taaaatgtat
    13201 ggaattgctt gatagagcaa atactgaaac ttatggaaca ccagaaccga ctcaggtatc
    13261 tatgaaaatc gagaaagggc catttattgt agttacagga catgaccttt atgatttaca
    13321 caaattatta gaacaaacga aagataaggg tataaatata tatactcatg gtgagttatt
    13381 accagcacat gcatatccaa aactaaaaga atataaacat ctaaaaggta attttggtac
    13441 agcatggcaa aaccagcaaa aagaatttga taatattcct gctccaatat tatttacaac
    13501 aaactgttta atgcctgtta aagatagtta tagagataga gtatttacta cagaagtagt
    13561 tgcttatcca ggtatggttc atattgatga agataaagat ttttctagtg taatagaaaa
    13621 agctcttgaa cttggaggat atagagaaga tgttcatatg actggtatta atggaggaga
    13681 tacattaact actggttttg caagaggtac agtttcatct gtacaagata aagtagtaga
    13741 tgctgtaaag agtggagcta taagacactt tttattggtt ggaggttgtg atggtgcaaa
    13801 acctggaaga aattattaca ctgaatttgt aaaacaagct cctaaagaca ctgttatatt
    13861 gacacttgca tgtggaaaat atagatttaa tgatttagac cttggggata taggtggctt
    13921 acctcgttta atggatatgg gacaatgtaa tgatgcttat agtgcaatta aagtggcagt
    13981 ttcattggca aatgcttttg aatgtggagt aaatgatttg cctcttagca tggtactgtc
    14041 atggtatgag cagaaagctg tttgtatact tcttacacta cttcatttag gtatcaaaaa
    14101 tatctatata gggccttctt tgccagcatt cttatctaag aatgtattag atatattggt
    14161 tgataaattt gcattgacac ctatatctac tcctgaagaa gatatgaaga agattttagg
    14221 ataaataaac atatctatac ttaaagatat ttatagttaa aatattttta agtatgccac
    14281 aattaagata aggggttaaa cttaaaattt gattaaaaaa tcaattttga gatgaccctt
    14341 tatttatttt ttgtaataat tgaaaaataa acctgctttc agtatattct ttttaagaat
    14401 ctttgtatta aatttataat acttagatat ataatatctt atatatttta ttttaagtat
    14461 taaaggtttg aatgtaactt aaatattaaa agaggaacat tgtaatatgt aacttgcata
    14521 aaatatatat actatagtga aattattttg attaaaattt aaaatataag gtgttttatg
    14581 tgggggtata tgtatatgaa aaattctaat tttaatatat tggcatttga tggtggtgga
    14641 ttaaaagggg cattgagtat atccatactt gagaggatag taaaagagta tccaaatcta
    14701 ttaaacaata taaatatgtt tggaggtaca tctacaggga gtctaattgc actagggctt
    14761 gcgtatggtg taagccctaa agagataaaa gaactttact caattgaaaa ttcaaagtat
    14821 atttttaata aaagttatgc agaaatactc agaccaaagt atgaaaacaa aaacttaaaa
    14881 gaagttttat taagtatatt tccagaggaa ctagaattaa aagatttaaa taaattagta
    14941 atgataccaa gtttttatat aggaaatgag gaaaatgcat ggaaaccagt gttttataac
    15001 aatatgccaa attcttttac aaagactagc aaagttgtag atgtagcaat ggcaagtagt
    15061 gctgctccgg tattttttcc aacatataat cgtcatgttg atggaggaat aatagcaact
    15121 gacccgagtt tagcgtgtat aattcatgcc attgacagtg gatttaaact taagaataca
    15181 agactttttt caatagggac tggatatgta tataacagta taaaggcaga tactaccgaa
    15241 tggggagcta ttgactggat aattaataaa gaacccgatt taccaattat atctattacc
    15301 ttagaaggaa attctcaaat gagtcagtta ttttctcaaa aacttcttgg agataattac
    15361 tatagattaa atcctaagat ggaaaaagat gtagctatgg atgattgtga tgcattagaa
    15421 tatctaatgt ctttaggaga aaattgtgat ataaaagatg cttttggatg gataaagaaa
    15481 aagagctttt atagaaatta gattcaattt gcaattgtat ttttacacat gatttcattg
    15541 agtaggtaaa aataataaaa tgcagattat taagttactt cttagggaat aaataaagta
    15601 taatttattt aaggagtgat gaaaatgata aaacttgcta ctttttcagg tggatgtttt
    15661 tggtgtatgg taaaaccttt tgataagtat gatggagtta ttaaagtagt ttctggatat
    15721 acaggtggtc atgttgaaaa ccctacatat caagaggtat gctcaggaac tactggtcat
    15781 ttagaagcaa ttcaaataac atatgatgat gatataataa gttatgatga acttctaaat
    15841 atcttctgga aacagattaa tcctacagat gatggtggac aatttggtga taggggaact
    15901 caatataaga ctgctatatt ttatcatgat gaagaacaaa aaaatatagc tttagagtca
    15961 agagaacatc tggaaaatag taaaatattt gatagtaaaa tagtgactga tataattcca
    16021 gcaactaaat tctacgaagc agaagaaaat catcaggatt attataagaa aagaccatta
    16081 aactacaata tgtattataa agggtctgga agatataatt ttgtaaaaag ccattgggat
    16141 ggaaacaata taaataggga agagttaaaa aagactctaa ctcctataca atttgaagtc
    16201 actcaaaatg atatgacaga agtacctttt gaaaatgaat attatgacaa taacaaagag
    16261 ggaatttatg ttgatattgt aagtggagaa gtgttatttt cttcaaaaga taaatttgat
    16321 tctggatgtg ggtggccaag tttttcaaag ccagttatag ataaatctat cattgagaga
    16381 agtgatttct cacaaggtat gtacagagtt gaagtaagaa gtgctaaagg aaattctcat
    16441 ttaggtcatg tatttgatga tggtccaaaa gaacttggag gtttaagata ttgtataaat
    16501 tctgcttctc ttaaatttat accaagagaa aatatggaag ctttaggata tggagaatat
    16561 ttaaaacttt ttgagtaatt aggaatattt attataaact tatctaaaag gtgaaaatat
    16621 aaaaaaatat tttcaccttt tgtttttttg tcaataatat gataaattat taattgtaat
    16681 cttatttata ccaatagatt aagaatttaa ttttgaaaga gagaaaacaa atgaatttaa
    16741 aagactttaa tggaaagaga ttaaaatcag ctagaatttt tagagcaaag acgatagaac
    16801 agttgtctaa agaaactaaa ataaataaaa aagatttaaa agcttttgag gaaaataagt
    16861 atgtaccaaa tatagaaaat acattaaaat tatctaatat attaaatttt ccaaaagagt
    16921 atttttataa aaatgaaaat ataaatatcg ttgtagagga tagccacttt aaccctcaat
    16981 ctaaactacc tagagtagaa gagatttctt atagagaaaa aataattata atacataaaa
    17041 tatattcatt tatggagaat tatattaagt ttccaaaaga aaacttgcca aatagaaaag
    17101 taatgtcaga tattaatata aatgatatag aatctcttgc atataagact agagagtttt
    17161 gggatttaaa tagtactcca atagtaaata tggtaagttt gttagagtct aaagggatta
    17221 taatatcagg tatgaatgta gatagaaaag gggcaactat ctttactcaa aaacagagaa
    17281 ttagtaaaga atctaaatat ctaatatctc tgggtaatga caaaaagtca gcatcaatta
    17341 gaaattatac tttggcatgt gagcttggat atataatatc aagtgaatta agaataccaa
    17401 gtaagcaatt ttcagaagat gagtatgcct gtgcattttt attgccaaaa gagagttttt
    17461 taaaagattt gactcatcca gaagatttag attattatgt agaactaaag aaaaaatgga
    17521 ttgtacctat atctgctatg atttttagag catataatct agaaaagata aattacaaaa
    17581 aatacaatta tcttatgaat gaaatggata aaaaaggctg gttgatagaa gaaccactag
    17641 acaaaatgaa aggttcaagc ccaacgtatt taaaaagagc agttgaatta ttgatagaaa
    17701 ataaaataat gagtgtaaat agtatagtat caagtttaga ggaatttgga ataaatcttt
    17761 atccagaaga cttagaatta ttgatgggat tgaaaaaagg attgttatct caagaggtaa
    17821 ataaaaaaag taaagttata aaatttgatg gtaaaaaata ggtttatatt ttagtattaa
    17881 aattaaaagc aggtacaaat gatttaaaca ttaacttagt taaaggtctt ttcaataagt
    17941 catttacctg cttttagtgt aagaaagaat attgaatcta aagtgttttt gaaagtgcaa
    18001 tatattccct taaaacagaa caagatttat taaattcatt ttgctcatat tcagtcatat
    18061 tgatttcaat aatttcttca ataccatctt ttcctataac acatggaaca cctgcaaata
    18121 catttctttc gttatattga ccttctagta atgtagaaac tggaagaact tttttctcat
    18181 tgtgtagtat agcttttaca atttctacta aagcagtacc tataccaaat tctgtacaat
    18241 ctttaccatt tattatactt atccctgttt ttttattttt ttcaactatt gatggtaagt
    18301 ctaaattgct ataagtactt gggttgtctt ttatcatgtc taagattggt ttaccaccaa
    18361 ttgaaacatg agaccaagga accatttgag aatctccatg ttcacccata gaataagctt
    18421 gaattgattg ttgagcgatt ccagtttctt cagataaaat ccttctaagt cttgttgaat
    18481 ctaaagctgt tccagttcca atgactttgt ttttaggaaa gccagatttt tcccaaatat
    18541 aatttgtaac tacatcaaca ggattagata tgactacaaa tataccatca aagccagaat
    18601 caactatggg ttttactata gattttatca ttccaatagt tggtcttagt aaatctaatc
    18661 tagttgtgtt ttgtctacgt aaaggccctt ctgaagagtc tgcaacactt atcactacta
    18721 tgtcagaatc tttacagtca gaaaaagttc ctttttctat atgtacttta tgtggcagat
    18781 atgaaactgc atcagctaaa tctaaagctt gagcttttga tttagattca tctatatcaa
    18841 tcataaagag ttcatcacaa actccttgag ttattaaact aaatccacaa tgagaaccaa
    18901 catgccctga acctacaata gatatttttc ttggctttat tgccatttta aatcactccc
    18961 cctaaaataa cgatacattg aaaattatag atgttattaa aagctgtttt tggatatttt
    19021 aatattctat tatttcagaa aatttattta atatgatttt atactaaaat ttttaaaaaa
    19081 gcaatgttaa tatactctct attagtgaat attaaagtat tatatgtata ttaataatta
    19141 ttaaaatatg taatgtcact gatagttaga atatttaagt gctaaaagat gtgtataaat
    19201 aataatgatg tcagtttata atgatattaa attgagatat actgtttctc taaattaaaa
    19261 aataaaggag taaatgtgtt atgaattatc atacaaaaga agaattggtg gaagcactga
    19321 gagtagtatc ttcaagtatt attaattgtg gaaaaggaca gaagaaattt tctgaggaga
    19381 catctcatca tacacgtttt aagaatatta tagaagtaat gtatatttca aaatcattaa
    19441 taatggatga aattagtaaa agagattgaa gcttattttg caatttaaca tgttttaata
    19501 tacattttat tatataagaa aataagtaaa catacatgtt ataaaatatt gaaataatat
    19561 acaattataa atttgttaac attaatatct atatgttaaa atatagtaga agatgttagt
    19621 aaataatagg atgagttcta ttttaataca attaaattaa gcttagtttg aaatttgtat
    19681 ctaaaaagta aagtaatcta gaattaagaa aatggagata gaattataat ggaaaaatta
    19741 tattatacaa atcagtatat aagagatttt acagcagaaa taattgaaat aaaagaagta
    19801 gaaaataagt atcatgtttt attagataag acagcttttt ttcctggtgg aggaggtcag
    19861 tttggagacc tgggaaaaat agagaatgat gatgtaataa atgtgtatga agatggaaaa
    19921 aaaatatacc atgtattaga taaaaaacct ataaagatac ataaggtaaa gtgtagtatt
    19981 gattgggata gaagagaaga tggaatgcat caacattttg gacaacatgt attgtctgga
    20041 tgttttttta aaacttttaa tgcaaatacg acaggttttc atttgggaaa tgagtatagt
    20101 acagtagata tagaaggtaa tttagaagag gataaaatta gagagataga gttgtttgcc
    20161 aatgaaatta ttcgtcaaaa tatacaagta gagacattac ttccaacgaa aaaagaacta
    20221 aaaaagattt ggttaagacg tgatttgcca aatactgatg aagagataag agtaataaaa
    20281 ataggtgatt tagatacgaa tgcatgttgt ggagtgcatc caaaatcaac tggtgaattg
    20341 agaatgataa aaataaagaa atgggaaaaa cataagtcaa gtacaagaat agagtttcta
    20401 gcaggtaaaa gggcagtgga tgaagtattg aagagggata tatatttgac taaaatatgt
    20461 agatatttaa gctgtagtga ggaagatgct ataaatggta ttgtaaatct aaatagtaaa
    20521 gtggaagagg ctttaattaa aaaaagaagg cttgaagaag tagtatctac atatcaggtc
    20581 aaagaaatga ttgaaaaagc aaataaggtt ggcaatattt cggtcattaa aaaaatttat
    20641 gatgatgaag atttgaaata tgtaaataag atagcaaata aaattgcaga atatgaaaat
    20701 agtattgcat tattagctgt taaatttaat gataaaataa atttagtttt tgcatgttct
    20761 aaaaatttaa aagatattaa tatgggagtt ttattaaagg atgcaataaa tcttatagat
    20821 ggcaagggtg gtggaagtaa agtattagct caaggaggag gtaagaataa tggaaattta
    20881 gaggctacac ttgattatgc atttatgaag atagaaaaaa ctctataaat atattatata
    20941 aaaaacctta gtagataaaa tagatatgga ggaataagta tggagcaaca aaaggactac
    21001 atagagttat atgaaaatga agaactaata gagataagag aagcacgtat ggatgatttg
    21061 gatacaatag caaaatttaa ttataatttg gctaaagaaa ctgaaggaaa agaacttgat
    21121 atggatgttt taactaaagg tgtaaaagca ttgttattag atgagagaaa aggaaaatat
    21181 catgtatata cagtatttga taaagttgtt gcacagatta tgtatacata tgagtggagt
    21241 gattggagaa atggaaattt cctttggatt cagagtgtat atgtagataa ggaatataga
    21301 agaaaaggta tctttaatta tttatttaat tatataaaaa atatatgtga taaagatgaa
    21361 aatatagttg gaatgagatt atatgtagaa aaagaaaaca taaatgcaaa agcaacatat
    21421 gagtctttaa atatgtatga gtgtgattat aatatgtatg aatacgaagt agtacattcg
    21481 taatataaat ttgtaatact gttatactga tgttataaag tattgattat attgacgatt
    21541 atatgaaata aactggatat tataattaga atttttaggg ggtataaaaa tgggcgtttt
    21601 aaaaggaatg ttttatgtat ttttattttt agatttagtg tcaatattct gtttcttttt
    21661 caataaagga aaaatagcat ctaataaaat tgtgtttaat gcaataggag tacttacatt
    21721 tgtactttgt tttatgcttt ttagttatta tcctaataat aatcttattg gaaaatttat
    21781 tgcttctcta ttttttatat ttggagttgc aggagttatg ttgaaagaaa aaaatttttt
    21841 atatgctaga cttcttttaa ctgtagttat agttttttct acattaagat tgttcgttat
    21901 tcaataaaat gtatttgtat aaaaatttat taaagtctac acatttaagt gtaggctttt
    21961 ttgatttttt gtttgaaaat taaggaaatt ttaaggaaat tttaaaatcg tcttaaatat
    22021 ttattgatat acttaaaata ttaatagcga aaggagactt atcagtgata gaaagtaaaa
    22081 agtcagaaaa attgttactg tcaattatat acataatatt tttggtatat attttaatga
    22141 ttataagaat aattttattt agagatgtac caatttacgc tattttcaag ggaacgttta
    22201 ggaatgtaaa tttaattcca ttttatacaa tatatcaatt tatagtagat agtaatcttg
    22261 actttatgaa agctactgta aatattatag aaaatatagg tatatttatt cctatgggaa
    22321 tttttttacc tatagtatgt aaaaatctaa ataaaaaaac tataattata gtaattatac
    22381 ttgttagcct agcatttgaa ttaacacaat atatttttgc tcttggaagt agtgatatag
    22441 atgatgttat tttaaattca ttaggtggca taattggaat taatatttac ataaacatga
    22501 ataaattatt tcccaatgat attaacagat ttaaggctat aataggtact agtttagttt
    22561 taggagttat aggattagga gttatttcta aaaattatca taacttacta acttttaaat
    22621 ttagacctga tagaaaaata tcaaaaatat taattgaaga aaatagggaa ataattaaag
    22681 atataaataa agatacagta gatatagttg ggacatttga gagctttaaa aaaggaataa
    22741 taactattaa agcaggttct aataacaaag taaagagacc agagaattta attgattctg
    22801 atggaaatat aagaatttat ttaaatgaaa atacaaaatt agtatcttat ataattacca
    22861 aagaatcaaa aatggatata gttaaatatg aggaatttga tgtgaagaat ttagatttat
    22921 taaggagata tgatactatt aatgtttgga ttgataaaga aaatcagcca aaggataaac
    22981 atggtattat ggctagtaaa ttattaattg gattgtatga ataaaatatt aaacaagaat
    23041 aaaagaataa caaatataaa aaacgaaaaa actatttcta taattatttt tacgttgtgc
    23101 ataaaataag aacattagtg aaggagtaga gataaattat ggagataata aaatatatct
    23161 tgtacttttt tatatattca tttttaggat ggacagtaga aagtatagga tgttctatag
    23221 caagcaaaag aataatcaac agaggttttt taaatggtcc aatatgtcct gtttatggtt
    23281 ttggagcggt aattgtgata tcactattgg gaagatttaa taatgtagta atagtatttc
    23341 tattaggaat gattttaact acgatactag aatattttac aggatttatt ttagaaacct
    23401 tatttcatgc taaatggtgg gattattctg acagaaaatt caatataaag ggtagagtat
    23461 gtttaaaaaa tgcaatatac tttggtgtaa tgtctgtttt aataataaga tttatacatc
    23521 catttataaa atattttgta agtataattc catatagaat attaatttca atggctataa
    23581 ttacaacact ttggactata cttgatttga ttgttactat tataacactt aaaaaattag
    23641 atataaagtt aaacttacta gacgatataa ttacagacct taatgatata aatgtgaaat
    23701 tggataaatt tgatagagga gaaatacaag ctttatttaa aacaataaat aacgacgaat
    23761 tagaagttag agaaaaaata aacaaaataa atagtaagtt ggaccgtata gagtctaata
    23821 ttattttaca aaaaagagtt ataaaagctt ttccacatat aaaacataaa aaacaacaag
    23881 agcaactaga acattttaaa aaaattatta atgagaaaag gtaattatac aatttaatga
    23941 agacgaatag aataatggta tttataagat gattttcaga ggactctata aaaatagaat
    24001 agagtccttt attttttgaa aaataatgtt tatttgataa aaataaaata tattgtttct
    24061 gaataacaaa tattttgaaa attataaaaa catatattga caatttgtgt caaaaatact
    24121 aacattaagt atataaagaa ttttatatga gaatatcttt ataaatcgca ataattttaa
    24181 aacataaatt tatttaaaga ggggatgatt tgccaatgag cgagataact agtcaaatga
    24241 taagtacaga agaaaaaaaa tatgttgcta aaactcaaaa gataccttat tatccagtag
    24301 catttaaatc aggtgatggt gcaatgctat atgattatga aggtaatgaa tatgtagatt
    24361 ttttagcaag tgcaggctct gcaaatgtag gtcatggtaa taaggaaatc tcacaagcag
    24421 ttaaagaaca aatggatgat ataacacaat atacacttgc atatttccat agtgatcctc
    24481 ctgtaaaatt agctgagaag cttgtggaaa tagcccctgg agataatgat aaaaaagtac
    24541 tatacagtgc aactggttct gcttgtatag atgctgctat aaaattagca agaggatata
    24601 ctggaagaac aaagataata tctatgtgtg aatcttatca tggaagtact tatggagcta
    24661 tatctatatc tgctttaagt acaaatatga gaagaaaaat gggtccattg ttaccagaag
    24721 tttatcactt ccattatcct gataaaaata gaacagctaa agaatgttta gatgagatag
    24781 aatatgcttt tgcacactat ctaccagcag aagaggtagc tgcaatattt atagagccaa
    24841 ttgcaggaga tgcaggaata atagtgcctc cagtagagtg ggtacaaggt ttaagtaaaa
    24901 tatgtaaaga aaatggaata ttacttgtaa gtgatgaaat acaacaaggt atgggaagaa
    24961 caggtaagtg gtttggtata gaaaattttg gtgttgaagc agaccttata gttcttggaa
    25021 aatctgttgg aggtggatta cctctaggag cagttgttgg aagaacagaa ataatgcaaa
    25081 gtcttgatgc accagcacat ttatttactt tagctggaaa cacaacagta tgtgtggctg
    25141 ctttaaaatc tatagagata attgaaaaag aaaacttact tcaaaagagt atagaaatgg
    25201 gagattatat taaagcagga tttgaaaaac taaaagaaaa atacgatata attggagaaa
    25261 taagaggcat aggattatct ataggagttg atatagttaa aggaaaaggt tctaatgaaa
    25321 aacatccaga tgctacagct aagatatgtt atagatgtat acaaactgga ttaataatga
    25381 tattcttagg acaatctact ttaagagtgc aaccacctct agtaataact aaggagcaag
    25441 tagataaagc aatgaacatc atagattctg caatagatga ttatttaaat ggtagaattg
    25501 gtgatgaagt atatgaagta actcaaggtt ggtaaaataa atattaaatg aaaataggag
    25561 ttatctcaat tttagaaaat tatttctgaa atgagataac tcttttaagt tacccacaaa
    25621 taagaaaatt gtttgattca ttaaagacta tatttcggat gtcgtttttg tgagtatcag
    25681 agacagtatt acgagaataa ttacctttat tcgtactatc tgttaattta gttataatta
    25741 agatagctaa aagaataaga attactgtgt ataaagcaat aacatatggt tttcttttat
    25801 atttgtattt gttattgaaa aaagaatggc cataattatt tttatttact ttagatgtat
    25861 ttgtattctt tttcttgtta cctttctttt tatcatcctt ttttctattt ttagggtaag
    25921 gaatgacttt gtgtgtatta tcaagtttga tatttttatt tttttgaata ttgttttcca
    25981 ggctagcctt tttttctcca aattgaatag ttttattggt attgttgtta tctttcaatg
    26041 ttaatctccc tcccttaaac tttcccttta gataatatta ctactaacct atggaatata
    26101 caataaataa tatataagta ataaaatttt ataggtataa atctctttct ccacgctcta
    26161 aggcagtcat attgttagat actatgtttc ttatatcttc tctttctatc ttacctattt
    26221 cttctgcgat aagtttttta cccataattt taagttcttc atctccataa tcgattaaaa
    26281 attcatttaa tgtagtaaga gcatttggtg tacatacatt atggatttgt cgacttttag
    26341 ctaagctcat gaatctatcg ccagttctac ctttacgata acaagctgta caataactag
    26401 gtatatatcc atcagtaatc agttctttaa gcacctcaac agggcttcta tggtctccaa
    26461 cttcaaattg ttctgtattg tcaccatctt catatgcttt atatcctcca acacctgtta
    26521 gagaacctgc actaatttgt gatacaccat attttagtaa ctcatttctc atttcagcag
    26581 tttctctagt ggacattatg attccagtaa aaggaacagc caatctagtt atagctacta
    26641 tttttttgaa catatcgtca gaaactaagt gaggaaaatc ttctaagctc ataccttcag
    26701 cttttttcaa tctagggaaa gatatagtat ggaaacctac accaaatttt tcctctaagt
    26761 gttcgttgtg catcataagt cctagaactt caaatttagg gtcagataaa ccaaataaaa
    26821 ctcctgcacc aacatcatca acaccagctt ccatagctct atcaaaagct gttaaatggt
    26881 aataatagtc attttttata gattgaccat gcatttttat aaatgttggt ttgtgataag
    26941 tttcttggaa taatatataa gttcctattc ctttttcttt tagtaattta tactcatcta
    27001 cacttgtagc tgctatgtta acgtttaccc ttcttatatt tccgttttca ttatatgttg
    27061 aataaattgc atctaagcaa tctaagatgt aatttatatc acaattttta gggtcttcac
    27121 cagcttctag agcaagtctt ttatgaccca ttttttctaa tattttaacc tcttccttta
    27181 tctcttccat ggtaagtttt tttcttttaa atttgttgca tttactaaaa ccgcaataaa
    27241 cacattcatt tacacagtag tttgatacat aaagaggggc aaataaaacc actctattac
    27301 catatatttc attttttatt tgcccagcta tactaaataa tctatctagt tgttttttat
    27361 cttctacttc aagaagtgta gctatatctt tataactaag tttttccctt ctatcagcct
    27421 tatcaagtac cttttcaata tcatcagaag ttgaattttt tgcatcttct aataaagaat
    27481 taataagttc atggtttatg aacatttaca aacgcctcca ttcaatatta tcaccgcgag
    27541 aatagtcaac ttcaagtcct aatgacttga tgttttcttc taaactttgg tgatattcac
    27601 aagcttcttg accagtaaag gctttgttat cgtaaagaga atattgtttt ctaaattctt
    27661 gaggagaaag gtttggcata attacattgc aaccagctaa aagacctgca tttctaccac
    27721 tagggtttat agatgccaag gctgttgtag cgggtaataa aacttttgga agtagaagtc
    27781 ttgtaattga aagcataagt aatgtcttct ctaaatctcc atgtttataa tccttaaaaa
    27841 ttgtatcatg gtgtggaata aaaggtccta ttccaaccat atgaggattt aactctttta
    27901 ggtataataa atcacgaaca aggtcttcgt tgttctgaaa tggtgattca accataaatc
    27961 cagcacctac ctgataacca atttctttaa ggttttttag gcattctttt ctagttctaa
    28021 gctcaatgtt tggcggatgt aattttttat aatgactgtc tgtagcagtt tcatgtctta
    28081 aaagatatct atctgcacca cataaaaagt attttttata cgaatcataa ctcttttctc
    28141 ctagtgatag agttatggca caatcctcat atttattttt tatacttgaa acaatttcac
    28201 atattttatc atcagtaaaa tatgcatctt caccaccttg caaaacgaat gttctgtatc
    28261 ctattttata acctatatca caacattcaa gtatttcatc aagtgaaagc ctgtatctta
    28321 tgatattttt attagaagat tgtattccac aatagtagca attattttta caatagtttg
    28381 taagctctat aagtcctctc aagtagactc tatttccata atgtttattt ctcatgtcgc
    28441 tagcttgttt ataaagatag tttcttaaat tttgttcgaa gttatattcc tcattgtcga
    28501 tattttctat taaatatagt agttcatcaa aacttaatga attaatgctg taaagtttat
    28561 ctataaagta tttaatctta tttttagtca tattcaaata tgtaattatt aagtatagtt
    28621 aaatttttaa taattgaaaa gttcaagtga tctttccaaa atacctttta catatgctat
    28681 taagacacca tagtttacaa taggtacatt gaaagatttt gccttttcta ttctagataa
    28741 cattccagct ctattcatca tacaagctcc acagtgaact acaagggcat atttatttat
    28801 atcttcagtg aaggatactc cagaactaaa ttcaaaagta atgtttttgc cagttttctt
    28861 tctaatcata ttaggaattt ttactgttcc aatatcatca gtttgtctat gatgggtaca
    28921 tccttcggcc atcaagattt tatcaccatc ttttaaattt tctaaagcat aagctccatt
    28981 aatcaattct ttaaggtctc ccttttgtct agcaaacaat atagaaaaag atgtaagagg
    29041 aatatctttt tgtgtatctt tatctacttg tggaaatact tgagagtcag taataacaag
    29101 ttttggtttt ttgcctaaat tagatagagt ttcttttaaa ctatcttctt ttgtaactat
    29161 agaaattgca ccattatcca aaatatctct tataacttgc tgttgaggaa gtataagtct
    29221 accttttgga gctgctttat ctattggaac aactaaaaca actaaatcat ttggttcaat
    29281 taaatctgat accaatttga actcagtgct atccttaggc aaaactttta ttatttcatt
    29341 ttttaaattt tcaattccaa ttttatctgt tgatgagaca gaaacaactg gacatttaac
    29401 tttattttta gttaagttta aaatttctga ttgattctta atagtatcaa ttttgttaag
    29461 aattagaata tgagggatat tcttatcatt aaatttttca attagtgaca aatcttcttg
    29521 tgatatacca atttggcaat ccactactag taaagctata tctgtctttt ctaaaacatc
    29581 tagtgattta cctattctaa gttcaccaag ttcaccaaca tcatcaagac cagcagtatc
    29641 tataagaaca caaggtccta taggaaggat ttccattggt ctaaatactg ggtctgtagt
    29701 agttccagca atatctgaga ctattgcagc actttgattt gttatagcat ttattatgct
    29761 agatttacct gcatttcttt taccaaagag acctatatgt actcttaccg attgtggagt
    29821 agaatttaga ctcatttgta ctcaccacct taaacttctt tttttgatat actagttttt
    29881 acagatacat gaggtatgtt gcccaattta ccagtcaaac tattaatagt gtccatttcg
    29941 ccaacaactg ctatacatac gattgagaca ccttcttctt cgcaaggaat gcccattctt
    30001 cctttaataa tacctttaaa acctgataca acctcattga atttaaattg acattctttt
    30061 ggttcttcca aaatagcact tataactgca acctttttca taaatatcct cctaactcag
    30121 aaaatatgtc ttaaacaaat ccaaaatcaa aaaacttttc tttgtaaaga aaagtttcta
    30181 caaaagaaaa gttattaatt acacaagtat aatgtgctat attaaactat ttccttacag
    30241 attagataac tcttttctgg tagggttagc ttgtaaaaat ttaatttaac aacaagtaac
    30301 tgaagaaaat ttttatacaa ttt
//
LOCUS       DAYASV010000034        29825 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYASV010000034 DAYASV010000000
VERSION     DAYASV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.034"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            115..1743
                     /gene="aspD"
                     /locus_tag="ACGI68_001550"
     CDS             115..1743
                     /gene="aspD"
                     /locus_tag="ACGI68_001550"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047688 - aspartate 4-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006531 - aspartate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HFH0137994.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1864..2859
                     /locus_tag="ACGI68_001551"
     CDS             1864..2859
                     /locus_tag="ACGI68_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFH0137995.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3037..3345)
                     /locus_tag="ACGI68_001552"
     CDS             complement(3037..3345)
                     /locus_tag="ACGI68_001552"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0137996.1"
                     /translation="MSKDVADEIEKTYAWKHTTTITLLARLTKKGFFISQRIGKHVHY
                     RVLIKEKEYLKIEGKRIFGGLHNNPLSELISKLHDGEEITEEKIIEIGNWIKSWKDED
                     "
     regulatory      3647..3744
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3885..4443
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3885..3914
                     /rpt_unit_seq="aatttacattccatatagttaatataaaac"
     gene            complement(4729..4887)
                     /locus_tag="ACGI68_001553"
     CDS             complement(4729..4887)
                     /locus_tag="ACGI68_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0137997.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5181..6551)
                     /locus_tag="ACGI68_001554"
     CDS             complement(5181..6551)
                     /locus_tag="ACGI68_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HFH0137998.1"
                     /translation="MKLKKLLSLGLVSMFVAGFPISANALDKIDKIQGADKYETAGII
                     ADKQSYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETFLKDKGIETRRLQGNDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGIESYAIGGTSSMSDSLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSNHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6767..7582
                     /locus_tag="ACGI68_001555"
     CDS             6767..7582
                     /locus_tag="ACGI68_001555"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFH0137999.1"
                     /translation="MNIKSAFIRKRGEKFYVYVEYIEETTGKIKQKSYGSYEKKKDAE
                     KHLIEIKSTINNNKFVAPKDMTFVERCYKYMDMNKNNWSPYTVTNTKSWVKNYIEPLF
                     KNLKLTEVNPSSVQAFVNYNFNNSTFTSAKVRYSFLSSVIREAYRLREIQENSCDFIK
                     LPSKEKYSTNEIYNRKEVLLLIEKLLSNNIEIPILLMLLLGLRMGEATGLRWSDVDLD
                     NNLINVNQILVYSNGKISFKEPKTPKSKRALSAPKELIEKLKIKKLRQSKLKL"
     gene            7612..7884
                     /locus_tag="ACGI68_001556"
     CDS             7612..7884
                     /locus_tag="ACGI68_001556"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0138000.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8247..8327)
                     /locus_tag="ACGI68_001557"
                     /pseudo
     CDS             complement(8247..8327)
                     /locus_tag="ACGI68_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(8367..9035)
                     /locus_tag="ACGI68_001558"
     CDS             complement(8367..9035)
                     /locus_tag="ACGI68_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0138001.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFSEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9888..12308)
                     /gene="leuS"
                     /locus_tag="ACGI68_001559"
     CDS             complement(9888..12308)
                     /gene="leuS"
                     /locus_tag="ACGI68_001559"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFH0138002.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDILDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12662..13012)
                     /gene="rsfS"
                     /locus_tag="ACGI68_001560"
     CDS             complement(12662..13012)
                     /gene="rsfS"
                     /locus_tag="ACGI68_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFH0138003.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(13129..13701)
                     /gene="yqeK"
                     /locus_tag="ACGI68_001561"
     CDS             complement(13129..13701)
                     /gene="yqeK"
                     /locus_tag="ACGI68_001561"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFH0138004.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13702..14391)
                     /gene="nadD"
                     /locus_tag="ACGI68_001562"
     CDS             complement(13702..14391)
                     /gene="nadD"
                     /locus_tag="ACGI68_001562"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFH0138005.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            14667..15305
                     /gene="recX"
                     /locus_tag="ACGI68_001563"
     CDS             14667..15305
                     /gene="recX"
                     /locus_tag="ACGI68_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFH0138006.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            15458..17461
                     /locus_tag="ACGI68_001564"
     CDS             15458..17461
                     /locus_tag="ACGI68_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFH0138007.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYEDMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITSPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELMKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDNGI"
     gene            17626..18276
                     /locus_tag="ACGI68_001565"
     CDS             17626..18276
                     /locus_tag="ACGI68_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0138008.1"
                     /translation="MSFLGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDG
                     LSRGAIYHHFKSKDEIIKAITSRLYNDGFYGYEVKNDEGKTALEKLQYRLLHTYEQHN
                     KQDIFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMEVKNPEIAAQMF
                     GFLLNTWCIPTVYPGNPQQMIERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            18369..19052
                     /locus_tag="ACGI68_001566"
     CDS             18369..19052
                     /locus_tag="ACGI68_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138009.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPIIDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRLGYQKDFDVTVPLLSQEQRDWLVNALITAYPDGHA
                     WIDQLANQY"
     gene            complement(19229..19540)
                     /locus_tag="ACGI68_001567"
     CDS             complement(19229..19540)
                     /locus_tag="ACGI68_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFH0138010.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSVSSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(19618..20436)
                     /locus_tag="ACGI68_001568"
     CDS             complement(19618..20436)
                     /locus_tag="ACGI68_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HFH0138011.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYIASNIHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFSGENKTIKENIKYSYL"
     gene            complement(20536..21060)
                     /locus_tag="ACGI68_001569"
     CDS             complement(20536..21060)
                     /locus_tag="ACGI68_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0138012.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            complement(21079..22221)
                     /locus_tag="ACGI68_001570"
     CDS             complement(21079..22221)
                     /locus_tag="ACGI68_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFH0138013.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKEYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            complement(22501..24888)
                     /locus_tag="ACGI68_001571"
     CDS             complement(22501..24888)
                     /locus_tag="ACGI68_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0138014.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGKMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYNDYKSDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKLNDKSNLSI
                     EQAKSKLNKIANDVGLDTINVAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQVKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAMYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(24881..25564)
                     /locus_tag="ACGI68_001572"
     CDS             complement(24881..25564)
                     /locus_tag="ACGI68_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138015.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDEPFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            complement(25681..26991)
                     /locus_tag="ACGI68_001573"
     CDS             complement(25681..26991)
                     /locus_tag="ACGI68_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0138016.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNVAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKSEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            complement(26985..27692)
                     /locus_tag="ACGI68_001574"
     CDS             complement(26985..27692)
                     /locus_tag="ACGI68_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138017.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            complement(27997..>29825)
                     /locus_tag="ACGI68_001575"
     CDS             complement(27997..>29825)
                     /locus_tag="ACGI68_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFH0138018.1"
                     /translation="YLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRYAEGDD
                     HIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYITPGNHD
                     FNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFFGLSTQ
                     ETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPNSIDIA
                     NEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEDIKIEN
                     KSARLIGKEEALKYKLDSVVSKKVDKIKEQQEKILDKVIGVTTNTLDGSYENVRTKET
                     NLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTKELTGA
                     QIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLNKKYVV
                     ATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIKSKDEL
                     ISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSSRESSNLGISKREA
                     KEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
BASE COUNT        10085 a         4830 c         3583 g        11327 t
ORIGIN      
        1 aatttcatac tttacttttt ctataaacct attataatta aagtataatc atttttgcaa
       61 cttgtttatt ttattataaa aatgtgaaac ttattacata aggaggaatt taacatgaaa
      121 aatcatactt ataaagaaat caaaaacata tatggaaaaa taagcccttt tgaatttaag
      181 gacaaattaa tagatattgc aaaatacact gcaaaagaaa ataacagaga attacttgat
      241 gcaggtcgtg gaaatccaaa ctggacttgt tcaactgcta gagaagcctt ttttactttt
      301 ggtcatttcg ctattactga aactcgttct aattgggatt taggtcattt ggctggaatg
      361 cctcaaaaaa agggtattaa agaaagattt tttaaattta taaatgaaaa tatagatatg
      421 cctggagcat atttagctag agatattata aactttggta taaatgaact tggatttgat
      481 ggagatgaat ttgttcatga actagctgat ggaattattg gtgataacta tccacttcca
      541 gatagaatgt tacctcatat ggaaaaaata gtacacgatt acctagttca agaaatgaaa
      601 tatgatatat caggtaaata tggtgatgta gaaatttttg ctgttgaagg tgctacagct
      661 gctatgtgct atatatttga ttcattaatg gcaaatgaac ttctaaaaaa aggagatacc
      721 atagccttaa tgactccaat atttacacct tatcttgaaa tacctaacct tcctcgttac
      781 gactttaaag tggtaaatat aaatgctaat gaagttgatg aaaaaggtgc tcatacatgg
      841 caatatacaa aaaaagaatt agagaaactt cgtgacaaat ctattaaagc tttattcgtt
      901 gttaatccta ataatcctgc ttctatagct atggatgaaa catcttgtaa taatctaata
      961 gatgtaattg aaaattataa taaagattta atgataatat cagatgatgt atatggaact
     1021 tttgtagaag atttttcatc cttaatgtca aaactacctt acaacactgt tggagtatat
     1081 tcttattcaa aatattttgg agttacagga tggagacttg gtacatttgc ccttcataag
     1141 aaaaatgttt ttgataagaa aataaatgat ttaactggag aattaaaaaa atcagtagat
     1201 aagcgttaca gtgatatgag tcttaaccct tctagccttt catttatgga aagagttgtt
     1261 gctgatagca gacttgttgc tctcaaccat actgctggtc tttctactcc acagcaagta
     1321 caaatggcgt ttttctctgc ttttgcattg attgacaaag tagatgccta caaaaaactc
     1381 aatatgagta tctgccatag acgtcaaaaa ctattatttg aagctttaga gcttccaatt
     1441 aatgaaaaca aaaacaatgc tgcatattac actcaatttg atattgaaga atgggctaaa
     1501 ttaaactacg gagaagactt atttaaattt ataagtaaaa ctgcatctcc agtagatgtt
     1561 ctttataaat tagctaatga ctattcagta gttttactta gtggaaatgg attctatggt
     1621 cctgaatggt caattagaat atctctagca aatctatatg atgaagctta tactaagata
     1681 ggaaaagcca taagagagat attaaatggt tatatcgttg aatggcaaaa aattaagaaa
     1741 taattatatt acactgaaat atctaaatta tatgaataaa agagtttatg tagaccttaa
     1801 ctactttttt tgatataatc ctatcggaat tataaatttt atttatgaaa ggcagagaat
     1861 agtatgagta ttaagaaaaa agttgccata gtttttactg gtggaactat atctatgaca
     1921 gtagatgata aagtcggagc agctatacca actttatcag gtgaacaaat actatcaatg
     1981 gttaccaaca ttgataaagt tgcagatgtt gaagttttta attttgatga aattccagga
     2041 cctcacataa caccttatag aatgatggaa ttgaaaaatt atgtaaatga tttattagca
     2101 agaaaagata ttacaggtgt tgtaataaca catggtacag atagtttaga ggaaacagct
     2161 tattttctag accttacaat tgacaacatc aaacctgtaa tagtcacagg tgctatgaga
     2221 agtagttctg aacttggata tgatggttca agtaatttat ctgcatcagt ttgtactgca
     2281 gtatccaaag atgctatggg aaaaggagtc cttgtagttt taaacaatga agtattatta
     2341 gcttctgaag ctactaaaac aaatactctt tctctaaata catttaaatc tctaactagt
     2401 ggcccattag gaataattga ctgcaacgaa cttgtcctta caagagatat tgtaaataga
     2461 acaattatag atactgataa ggtagaatct aaagttgcct tatttaaagc atatgtagga
     2521 gaaaatgctg actttataag atttgctgtt gatagtggtt ataaaggtat agttattgag
     2581 gcaatgggta gaggtaatat tcctcctcaa atgctttctg gagttgaata tgctagagaa
     2641 aaaggacttc ctgtagttat agtttctaga tgtcattcag gtagagtatt cgatagttat
     2701 ggttactttg gttctggtag agatttaaaa aatcttggtt gtatatttgg tggggactta
     2761 ccaggtcaaa aagctagaat aaaacttatc ttagcacttg gaaaaactga taaccttgat
     2821 gaaatcaaag atttctttga aaaaggaatc tattgttaat tctaataact tctatttaat
     2881 tatttattga tatttcaaca tttttaaatc aaaatctaat acttatgtca gttaaatgtc
     2941 agcaaaatat tttatccaca agttttccac aacttatccc caatattatc cacaagaaaa
     3001 aaggtagcac ttatattagt agctaccttt tatttattaa tcttcatctt tccagctttt
     3061 tatccaattt cctatttcta ttattttttc ttcagttatt tcttctccat catgcaattt
     3121 tgaaattaat tctgataatg gattgttatg tagacctcca aaaattcttt ttccctctat
     3181 tttgagatat tctttctctt ttattaatac tctatagtgt acatgtttcc caattctctg
     3241 cgatatgaaa aaaccttttt tagttaatct agctaaaagt gtaattgtag ttgtatgctt
     3301 ccaagcatat gttttttcta tttcatctgc aacatctttt gatattacag ttttataacc
     3361 agtattctat atgtatttca taatttttaa ttctgcatct ggtaattttt tattcaacat
     3421 aatatattcc ttctttcaat ttatgtcact tcctcaaact tatctagctt aaattaataa
     3481 taggagcttt ttatcacaaa aactacaggt aaatttttct atatattcta cgtatttagt
     3541 ctgtttttta agtgtgaaat tacgttcata cctataaatt tattcaattt tcaaacaatc
     3601 ttattgaata ataactaatt ctattgtatt atttagttat agaataaaac taaatcggca
     3661 aaactagaga aatctagtga cgcaaagcta tagagactaa gacttatata aatatcttat
     3721 gagttatgtc agccagttgc caaagagata ttgttctttt tgtttttatg aaagtttttt
     3781 agggggataa taatttatat atttagagat aaaatagata aatgtacaca tatattgact
     3841 gcttatatta gcagttcgta tgattattgt aattttttgg atacaattta cattccatat
     3901 agttaatata aaacaagtgt ttcgaatttc ttttctgaaa ctataccaac aatttacatt
     3961 ccatatagtt aatataaaac gttatatact gaaagcggtg aaactggtta cgtagcaatt
     4021 tacattccat atagttaata taaaacctgt gcgtgtagga gatggtaaca caactgttga
     4081 agaatttaca ttccatatag ttaatataaa acacacatct acatgttttt ctatctgagt
     4141 tcatctctaa tttacattcc atatagttaa tataaaactt tttaatcctt cttctgaact
     4201 cccaaagttt atatcattta cattccatat agttaatata aaacccacga aatttaaggt
     4261 cgatatatca gatttgaaaa aatttacatt ccatatagtt aatataaaac aagctaaatg
     4321 gagcaaccga ccaagtcgct agaaataatt tacattccat atagttaata taaaactttt
     4381 ctattaagtg taagcagaat gcatggaata tgaaatttac attccatata gttaatataa
     4441 aaccccaaaa taaattgagt atttccaatg cctacacata tacaaatctc tcaaatttgc
     4501 agtgaaccat gagtagtgca attgataaca cttatcacaa gtcctcaatg ccttatattt
     4561 caactgttaa gccttaattt cttcaaaaaa tcgaacactg caaaatttct atgactttat
     4621 tatatcataa aaatattatt tttgaatatc tgtaccaatt tgtgatataa taaaagcaag
     4681 aagaactaca atctattttg ctgtagagtg gagttcataa tttagaaatt acttagtttt
     4741 atggaatctg atttttaaat caaattccca accactctta gttgccgctt tgagtggttt
     4801 tttacgcttt ctaaatagtt tgctagctat ataaactatt aagctagcag atagacttgc
     4861 tagtatgcct tgtaaaaaat tatccatgta tattcacctc ccttcttaac gtcgggagga
     4921 taattttttg atacatgaac tccactctat aaattgtaga ttacatcttc ttgctaaaaa
     4981 tattataaca tataattctt acatatttta cctatttatt actttttaca ttataataat
     5041 attcttttaa tcaaaaaaac acctactatt aaagtaaatg tttttaagta tttttaattt
     5101 taaaatccat atagttcata tgaaactaaa tataataact caacatatga gcaatatcga
     5161 gacatttaca tacccactag ttatttatat tcaaaaaaat cttctccatc cgggttataa
     5221 aataatattc tacttgccaa tccttgttca tatttataat tttctttatc acctacataa
     5281 gaaaatgtta atgtcatgct tcttgatgga tctaatgaaa ttatatctaa ataaatagta
     5341 catttttctc ctggctttaa tacaccgaaa ctattaaaat ccatttttat tttagcgtat
     5401 tgccccatat ataatgttga attagcttct ccataaactt ttaattttgt tacatctaaa
     5461 ttttgtgtac ttgtattttc tatatttata ggaactgttg ggtctttata tttttcagta
     5521 taatattcaa ggttatcatc ataacattca agataacttc ctttatcttg ccaaattatg
     5581 tgatttgaat caaaatcaat ctttaatcca catattatat ttttatttga agatggaaga
     5641 ctaatattat tgatggtatt ttcacactct agcataattt tatcacttag atttcctatc
     5701 ccggttactt ttgcagcatt tttaagtata tctttattac ttccaatatc aactagtaca
     5761 ataggattat ttttagccat tggtgaagca actaaagcat aaactaaatc agtaccacta
     5821 gctatataaa attcttttgc gccattatag aacttattta ctattttttt attagtatca
     5881 tatctatcta ctccacccaa tcgagttgaa ttagtatcat ttactaaact atcactcatt
     5941 gatgaagtgc cacctattgc ataactttct ataccagttg tattaaatgg aacacttttt
     6001 ccatcagtta atactatagc tgctttatct ctaactgcta caggtgcagc actcatagca
     6061 tctggctcac ctttgaaagc gttagttaat attactttat ttactttatt aatggaattt
     6121 atttcttttg ctacattata acttgtctta atcctatcat taccttgaag ccttctagtc
     6181 tctattcctt tatcttttag aaaagtttct gttgctttat caatggaact ttctcctcct
     6241 attatgtata cctttttagc tttttctact cttttaagag ttgcattagg tatattattc
     6301 tttttagtca aaaggattgg tgcatttgtt gctcctgcaa gtccactcgc acttagccca
     6361 tcagccattg tactatctgc atttataagt attgcagtag tataactttg tttgtcagct
     6421 atgataccag cagtttcgta tttatctgct ccttgaattt tatcaatttt atcaagtgca
     6481 ttagctgata taggaaaacc agcaacaaac attgacacta accctagtga taataatttc
     6541 tttaatttca caatatctcc cccttttata tatgaaatac tatatatctt aataattcta
     6601 cattaaattt tcttttcctc ctttaacgat atttatcttc ctgttaattt aagtgtatac
     6661 aaatacgtgt tataatgtaa ctgtaaagaa ttaaaaagat aatgacataa ttagagtagt
     6721 acaactgttc ttttataaaa ctaacttata aagggagcgt acatatatga atatcaaatc
     6781 agcttttata agaaaaagag gggaaaaatt ctatgtatat gtggagtaca tagaggaaac
     6841 aactggcaag ataaaacaga aaagttatgg aagttatgaa aagaaaaagg atgctgaaaa
     6901 acatttaatt gaaataaaat ctactataaa caataataag tttgttgctc caaaagatat
     6961 gacatttgta gaaagatgtt ataaatacat ggacatgaat aaaaataatt ggtctccata
     7021 tactgttaca aatacaaaat cttgggttaa aaattacata gaacctcttt ttaagaatct
     7081 aaaactcaca gaagtaaatc ctagttcagt acaagctttt gtaaattata actttaataa
     7141 ttctaccttt actagtgcta aagtaaggta tagctttcta agttctgtaa taagggaagc
     7201 atatagatta agagaaatac aagaaaattc atgtgatttt ataaaacttc cttctaaaga
     7261 aaaatattct acaaatgaaa tatacaatag aaaagaagta ttattgctaa tagaaaagtt
     7321 acttagtaat aatatagaaa tacctattct tttaatgtta cttctaggtt taagaatggg
     7381 agaagctact gggcttaggt ggtcagatgt tgacttagat aacaatctga taaatgttaa
     7441 tcaaattctt gtctattcta atggcaaaat atcttttaaa gagccaaaaa caccaaaatc
     7501 aaaaagagct ttatcagctc caaaagaatt aattgaaaaa ttgaaaatta aaaaactaag
     7561 acaaagcaaa ttaaaattgt aaggtatcct ggaaaacaga aataatttag tttgtctaaa
     7621 caccaattta aatccgtttc taccttcttc actaagaaaa acgtttcaca attttacaaa
     7681 aaaaataatt taaaaaagat tagaatacat gatttaagat atacaagtgc cactctgctg
     7741 cttttagggg gaactaatat gaaagttgtt tctgaaagat tgggtcatac agatataaaa
     7801 ataactatga atagatattc tcatgtactt aaagaaatgg acaaagaagc ttccgacaat
     7861 ctaagcgaac ttctatttaa atagtaaaaa ctgactgtca gttaaatgtc agttaaacct
     7921 aaaaaaatag gttttgtcag ttgattatca gtcaattatt ttataatagt ttcacttaaa
     7981 aacgtctata tctacacatt ttactatttg tttatgctta ttaatatcta tctgttatta
     8041 ttaattctaa tagcttctat ttaattattt agttcagttt acaggaaact acacacaaac
     8101 cataaaatag gatgttgaca atataacaga tagtatgctc ctgtaaaagg taacgtacta
     8161 tctgttatgt tgtctggatt tccaaatggt taccctttgg cacacaactt tgctatctcc
     8221 ttggtaaact aaaagtttgt aatctaaaat cctaatccat ctctataaaa tttaattgac
     8281 ttctccatac tttttacacc taaacatata aaagtaattt tattcactta atgacctcca
     8341 taattcccaa attagctatt tggaacttaa ctaaaaaatc ctaatataag ggcaactatt
     8401 ataatggcaa tcggaataca tactgtgtaa tcctgttcca tagagtcatc atctgaatgt
     8461 agttttacaa ttcgcaattt agcaatcagt ttgaagcaga aatagcctat tattgttcca
     8521 attacattcg tgagtaagtc gtctatatcg gttgctctgt gtaacgtaaa aagctgaata
     8581 gtttcgataa aaagagataa tccaaaacca atcaagaatg tatttctcaa acgttcaaag
     8641 gttttgctga taagcggaca taaaaaacct aatgggacaa aaagtaaaat attcaaaata
     8701 aaactcaaac taaatccatc actaaaagga ataagattta tgtttggatt aaagaacgtt
     8761 tcccctaact gtgacagtct tatatattca ctgaatgtag ggataccaac aatatgcgat
     8821 agtgttaaac ataagtagta atacattgcc agtgataaaa tagatgtttt tacctttgga
     8881 atatcactat ttttcttgaa tattacaact aagaaaatac caatcaagat aattcccaac
     8941 aagaaaatag gattaattaa caataaatct ttgatagctt caaacagact gagttgaatt
     9001 tctacacctg attgattatt tgttacagtc tccatttgta catacctcct cataacattt
     9061 caattatttc taaaacacaa aatagaagat aaatcttaaa atcatcctat caaaattttt
     9121 aaatttgcct ttatcagttt ataccaccag ttaattcctg tcttacttct gtatggtatt
     9181 cttgggattc tttcatcaaa ttagacaaaa aatggttacc atatcaataa actggttacg
     9241 ctccatatct cccgaagcaa ccatcccctg agaataaatc cgtatcagga atctccacat
     9301 ctactgcacc tatcacaagt tatttgataa catcctcaga caatcccaac ttggatatat
     9361 cgacaacacc cattagaata ccactgctga ccatacttgt tttcctgctg ataattttaa
     9421 tcaactggga agttgaaacg ctatcttttt tacaagttcc tttttagaaa catggtagct
     9481 attatatgaa tctgaatttc gttgtctggg tgtttcatat aaagcataga tacgcacctt
     9541 ttttgcctgt tttcattata tagaaaaaaa tttcaaaata caataattat atttttttcg
     9601 tttaagctta gagcagttcc ttgatgaaca acactttgga aactgaatga tttacatacc
     9661 acttaataca ttaactcgat ttaacattaa ttataataat atttaaaaca acaactaggg
     9721 tatataagaa tgaacttttt agttctttaa tcattttttt gtataaaatc aaatctattt
     9781 ttataatttc aacaatttta taatttcaac aatgaaaaaa ggcttatctc tattttgaga
     9841 cagcctcgtt tattttataa atttatattt ttgttcatat ttttgagtta tttaacaact
     9901 atatttacta gttttttagg tacagctaca actttaacta tagttttacc ttcaactaat
     9961 ccttttattt tttcatcttc taaagctacc ttttccatct catctttaga tatatttgag
    10021 ttaacagtta attttcctct aacttttcca ttaacttgta caacaacttc tatttcatct
    10081 tttactaaag ctttttcgtc atatttaggc cagtctatgt caaatacact tccttcttta
    10141 cctatcatag tccatagttc ttcacctaaa tgaggggcaa atggtgcaat tatagttact
    10201 atagtttgta caccttcttt tataactgct tcgtttatat tatctaactc tttatattta
    10261 tacatatcat ttattaattc cataagagct gatatagcag tattaaatcc gaatttttca
    10321 cttaaatcag cagtaacttt tttaagtgta gagtgtatag tatatctcat atccttatct
    10381 tgggaattta attctccaaa ctctacatcc tttttaacca catctgctaa ctcgtcaacc
    10441 aatctataaa ccctatttaa gaatctaaag catccatcaa caccttgctc tgaccagtcc
    10501 aaatctcttt ctggtggtgc tgcaaataat acgaaaagtc ttgcagtatc tgctccatac
    10561 tcatctatta tatccatagg agatactgta ttccctttag acttactcat tttagaacca
    10621 tccataagta ccattccttg agtaagtaag tttttaaatg gctcattgaa gtctaccatt
    10681 cccatactct taaatgcctt tacaaaccat cttgcataaa gtaaatgcat tatagcatgt
    10741 tctactccac ctatatattg gtctactgga tgccatctat taactaattc cttactaaat
    10801 ggttcatttt cattattgct atccacatat cttaagaaat accaagaaga atcaacgaat
    10861 gtatccatag tatcaacttc ccttctagct ggttttccac agtgaggaca agttgttgac
    10921 ataaattgct ttgaagtcgt aagtggagat tccccttttc cagtaaattc aacatctgtt
    10981 ggtaaaagaa ctggtaaatc ttccttttta actggaacta ttccacattc atcacaataa
    11041 accacaggta taggacaacc ccagtatctt tgtcttgaaa ctaaccagtc tctaagtcta
    11101 taattaattg ttctctttcc tctaccttct ttttctaact tatcgattat tccttcaaat
    11161 ccttctgaag catctaatcc attaaattct tctgaattta tcattttgtt atcttctgtt
    11221 attacatcaa ttatgtctaa attatatttt tcagcaaaat ctctatctct ttcatcatgt
    11281 gctggaactg ccattatagc acctgttcca tactcaacta atacatagtt agctatccat
    11341 agaggaactt tcttgccatt taaaggattt ataacatatc tacctataaa cattccttct
    11401 ttttctacat cagctgctgt tctttctatt tctgtcatag tatgcatttt ttgaacaaaa
    11461 gcttcaacat ctgcttcata ttcagtccct gcaaccaatt ctttaactaa ttcatgctct
    11521 ggagcaagta ccatataagt aactccataa gttgtgtctg gtctagttgt aaatactgtc
    11581 atagatttat cagttccatc tatatcaaaa actaaatctg ctcctgtact tttacctatc
    11641 caattttttt gcattgtttt aactttttct ggccatccat ctaatatatc taagtcatta
    11701 agtaattctt ctgcaaattc agtagtctta aaataccatt gttctaaatc tttctttaat
    11761 actgttgctt tacatctctc acatgctcct tgaacaactt gctcatttgc aagaacagtc
    11821 tcacaagaag gacaccagtt aacatatgat tttttcttat atgcaagccc atgctctaaa
    11881 aacttcaaaa atatttcttg tgtaaatcta tagtattctg gcgttgaagt tgcaacttct
    11941 ttatcccaat cataactaat acctaataaa tttaattgct ctttcatttc ttctatattt
    12001 tccattgtcc atttatgtgg atgtattcca tgttttatag ctgcattttc agctggtaat
    12061 ccaaaagaat cccatcccat tggatgaagt acattatatc cttccatttt tttgaatctt
    12121 gcgacaacat cacctataga ataatttcta acatgtccca tgtgtatttt tccagatgga
    12181 taagggaaca tttctaatgt gtaataatta ggtttttcag tttgtgcagt atccatttta
    12241 tactgattat tatctttcca gattttttgc catttcgatt ctacttcttt aaaattatat
    12301 acgctcatat tttgcctcct atttttaatt gtgtataact accttattta gtattattta
    12361 cttataattt ccaaataatt ctttgaaaat aaaaaatcct cgtcactaaa cttacgttta
    12421 gggacgagga taaatcttcg cggtaccacc ctaattagcc tactacatat atagactctc
    12481 ttagatagct tttaatccag ccatggagaa tagattttaa tctagtaagt tcaaagttat
    12541 ataatattag ttctcacctt ccactaactc tctgaaaaaa tataatcttt tactgctcta
    12601 gtctattcaa tttaatataa tttttgaaat tttctaatta taataacata tattatcatg
    12661 tttatgctaa tgtgtcaata tctatataag gtgcatcttt ccataatttt tctaaattat
    12721 aaaaccctct attttcctca ttaaatacat gaaccattat gtctccataa tcaaggagta
    12781 cccaagtttg agttccttgt ccttctttac ctcttggctc taatcctagt tttgctaatt
    12841 catcttccac attgtcagca atagctttta cctgtctctg agaagatgct gtagtaataa
    12901 taaaataatc acacaaacta gaaacttttc ctatatttat tataactgta tcttgaccca
    12961 gtttatcttc tatggcatca tatgctattt ttgtcatttg ttccactgtc atataaaaat
    13021 tcctcacttt cctaattaaa atacacttag ttattgtaac atattaatac gattttgtaa
    13081 catagtcaaa tttaaattta ttaatatgtt tgcctttata tagctttttt ataataactt
    13141 ttctttaatt agatagtttc ttgccataac tgttcttgga tgtatctctt cttttttatt
    13201 gatgacaaac attaaagtat tattaaaaga agttaaaagt gcttcatcaa gcttccctcc
    13261 atatgctaat tctcttaatg tatctacaac tgggaatttt cttccttctt caattaaatc
    13321 agctatgtaa attatttttt ctaaaagaga catgtcctct ctccctgttg tatgatattt
    13381 gattgcattt ataatatctt catcttccac ttcaaattca tactcgcata tgtacgcccc
    13441 aattacacta tgagacaagg aacgatttcc atcctctatt gggtctaaat atatttcata
    13501 cttatttaca taatcctcta tttccttgtc acttaaatac tttgcacaat catgaatcat
    13561 tcctgctaag taagcttttt ctttgtcaca tccataaatc tcacataatt tttcagcaca
    13621 ttttgcaaca ttttttgaat ggctaagcct ccccactggt agcatttgat ttagtctttg
    13681 gttaatattt tctaagttca tttaatcatc tccatattga tatagtttat tttcatataa
    13741 ataattttct acattttcgg gtactaagta acgtattgac ttgccttcat ttagctgttc
    13801 ccttatatat gtggacgata tatccagtga tggaacataa actgttataa tgtcagcatt
    13861 atacttctta gttagttttt ctatagtctc ttgtgacctt aacaaactaa ttcctggtct
    13921 agttgcggct ataaaagtag ctaactcaaa gtttttcttt acatctttcc actcttcaac
    13981 actacaaatt gcatctgcac ctgttataaa ataaatgtct gcatttttgt attttttctt
    14041 taagtatttt aatgtatcta ttgtataagt ccttttcttt ctttcgattt ctacttttga
    14101 aactaaaaaa tcttcgttgc tttcagttgc tagaagtgtc atattatatc tatcatactt
    14161 atctgtagtg atacataatt tatgtggtgg atttcctgat ggtataaaga ttattttgtc
    14221 aatgtcatac ttgtctctaa taaattctgc tgttgctaaa tgagcatagt gtatagggtc
    14281 aaaagtacct cccaatatac ctattttgat tttgccttta tggcgattaa atttctctag
    14341 tttttcagta tttttcaact ctgccatctt aactagattt tcacttctca tattttgtct
    14401 ccttttaatg ttataatact attgtaccat tattttttat ttcttaaagc ttataatttg
    14461 tttttttggg tatataatta tatttatatg ctaaaaaaca ctttaaaaaa taattattta
    14521 acataattct gctttacttt ttaataaaaa taacttttta ctattttgtc ttaattttga
    14581 taaaatataa catacaaata taagtttttt gttaaatttt attagttgct tttgttaata
    14641 taattacata taaaaaagga ggacttatgg gtataattac aaaaatagaa caacaaaaaa
    14701 gaaatgatga tagagttaat atttatgtag atgataaatt ttttattgct atatttaaag
    14761 aactggttta tacatttaat ttaaaaaaag ggaatgaaat aaatgaagaa gaattaaaac
    14821 ctattttaga tgatgaaatg tatataaaag ctaaaaataa agcattaaat attttatctc
    14881 atgctgacca atctgaaaaa aaattaaaag aaaaactatc atcagaattt gatgaaaata
    14941 taattgagag ggtcatagat tttcttaaaa gttataatct cgtaaatgac agtgtattag
    15001 ctcaaaaaat tgttaacaca aatgttaacc taaataaatg tggcaaaaac agaattaagc
    15061 agaatttata taataaaggt atcaatagaa gtacaatcaa tgaagcagta tctgaattag
    15121 acaaggacac tgaatttgaa aatgctatgt atctagcaaa aaaaaggtat gaaagagtaa
    15181 agaaagaaga taaaaagaaa atatatcaaa aaatttctca acatctttcc tataaaggct
    15241 ttgattatga tattattaaa agagttttaa acaaactttt aaattttgat gaatacgata
    15301 tataagtata aatactaggg aggtaaatta tgaaattaat cattggtggc ttaagctttt
    15361 tattaggatt atataatata tatcaattat acagagaaga tagtatgaga taaatattat
    15421 tttttagatg taatatttat tatggaggag aaatacaatg aaaagtcaaa aaagatttaa
    15481 aattttagat attgatgtat acctacaacc atttgaatct gatataaaaa agcgaatgaa
    15541 acattatgag gatatgaaat caaaaacatt gaaaaactat aaagatttta gttcttttgc
    15601 aaatgctcat ctttactatg gatttcatca gacaaatgat ggattcttct atagggaatg
    15661 ggctccaaat gcagatagtt tgtcactaat tggtgacttt aataattgga atagaaaatc
    15721 tcatcctcta agaaaaattg ggtctggtaa ttgggaaata tttattcctg gtcaaaatag
    15781 cttacctcac aaatcagagg taaaggtaca agttactgca aatggaaaaa catttgaccg
    15841 tataccatta tatataaaaa gagtaattca aaaagatttt ggtggattta atggacaaat
    15901 atggcaacca aaaactccat ttatctggac tgacaatgat tttgacttaa aaaacataac
    15961 ttctccacta atctatgaat gtcatatagg tatgtctact gaaagcgaat ctattggaac
    16021 ttataatgaa tttactgaaa aaatacttcc taagataaaa aaagctggat acaataccat
    16081 tcagcttatg gctattatgg aacatccata ttatgcatct tttggatacc aagtttcaaa
    16141 cttttatgca atctcatcta ggtttggtac tcctgaagac ttaaaaaacc tcataaacac
    16201 tgctcattca atggggattg ctgttttatt agatttagtt cattctcatg ctgtaaagaa
    16261 tactttagaa ggaataaatg agtttgatgg aagtgaacat caatttttcc actcaggttc
    16321 aaagggaaat catcctgctt ggggtacaaa actttttaac tatggtaaac ctgaagtaat
    16381 acattttttg ctttcaaata taaaattttg gttaaatgag taccattttg atggctttag
    16441 gtttgatggt gtaacttcaa tgttatatca taaccatggt cttggagttt catttgatag
    16501 ttatgaaaaa tattttagta tgaatacaga tattgaagct ataacatatc tccaatttgc
    16561 aaatgaatta atgaaagaaa ttaaacctaa ttcgataagt attgcagaag atatgagtgg
    16621 aatgcctggt atgtgtatcc ctataaaaga cggcggaatt ggatttgact atagactcgc
    16681 aatgggagta cctgattttt ggataaaaac tatctctaac ttatctgatg aagactggga
    16741 tttgggcaaa atgtggtatg aacttactac tagacgacct ggagaaaaaa atataggata
    16801 ttgtgaatcc catgaccaag cattagttgg cgataaaaca attatttttt ggctagctga
    16861 caaggaaatg tactggaata tggaaataaa ctcaaataac catgttatca atagagcaat
    16921 atctcttatt aaattaatta aattaataac tttttctcta gcaggtgaag gatatctaaa
    16981 ttttatggga aatgaatttg gacatcctga gtggattgat tttccacgag aaggcaacaa
    17041 ttggagctat aagtatgcca gaagacaatg gagcctttca gaagataaca acttaaagta
    17101 taaacaatta ttaaattttg ataaatctat gctcgaactt acaaaataca gtgatatttt
    17161 tatccaaaat agtcatgaac ttatacatat ggataatgat aaaaagattt tggtgtacag
    17221 taagggcaaa tatttattta tatttaattt ccaccctaat ctaatacaat cagttgatat
    17281 ttcaaagttt caaaatacaa actacaaaac gatattaaat actgatatgg tagagtttgg
    17341 aggaaacaca aaaaaagaaa acttaagaga ttatgatgta tactttagct caactcctcc
    17401 ttcttctaaa atgcctattg cctctcgtac agcaatagtg cttttagata atggtattta
    17461 aatatgagcc aaatatatta ggaaactgca aatatagtcc agtataaggc aaaaaaataa
    17521 aataatgaaa gcatggcaat atacctgttc aacatgctaa aaagacacat tgacaaatca
    17581 ttatgtattt gctaagataa acataccaaa tgattgtttc gaggagtgag ttttttggga
    17641 agaaaaaaag aacctgataa tactatacaa aaaatacttg atatttcaca taagttattc
    17701 ttagaaaaag ggtatgagca gacaacgatt caagacattg tagataattt agatggcctt
    17761 tcaagaggtg ctatttatca tcactttaaa tcaaaagacg aaataattaa ggcaatcaca
    17821 agtcggttgt ataatgatgg attttatggg tatgaggtaa aaaatgatga aggaaagacc
    17881 gcattagaaa aattacaata taggttgctt catacttatg aacagcacaa taaacaagac
    17941 atttttttaa tgtcatcatc actattgcgt aatcctaagt atttagctac gcagtttgat
    18001 gagattataa atgatattgt gccttggata aaagcattga ttgaagaagg aaatcaagat
    18061 aattctatgg aagttaaaaa tccagagatt gctgctcaaa tgtttggttt tcttctaaac
    18121 acatggtgta ttccaacagt ttatccaggt aatccacaac agatgataga acgttttact
    18181 ttgtttggtg agcaagctgc ttttatgggt atttctttta tgaatgataa gataatgtgt
    18241 gaatatcaaa aatttgttaa taaattttct aaatagtaaa tataaaactg ccttaatatc
    18301 ggcagtttta tatttacaca aaacaaacat ttagttggta ttttaaaaaa tttaatttat
    18361 ttcagtacat gcaaaaggag gttgctatga acaaactaat taaagattgt gtaaactttt
    18421 ttaatgattg tggcttttca tattcaatat gtggtggata tgccctagaa ttatttgcga
    18481 atatgcatat tcgcccacac agtgatattg acatatggat ttctgacaca gataaagaaa
    18541 agagcattca gtatatgctt aatcaaggat gggatatata tgagcccttg ggtaattatt
    18601 tattacgacc tattatcgac cttaacaagc aacatataga gcaaatttgt gtttttgcga
    18661 ttaagcctga ttgctcattt attgaattaa aaaaagtcga ggatgataac tataaaatga
    18721 atatacagtg tgacgaacag ttacagttcg attttattga attgatatat aatccacaga
    18781 aagatggaaa atttcttttt tcccaaaatc aaagaattat aagagatatg gataaggcca
    18841 gactgataac atcagataat atcccatata tggctcccga agtggttttg ttttgcaaat
    18901 caatatacat agataggctt ggttatcaaa aagacttcga tgtaacagtc ccactattat
    18961 cacaagagca aagggattgg cttgtaaatg ctttaataac agcatatcca gatggacatg
    19021 catggattga ccaattggca aatcagtatt aagcctttta gctcccgaat atgacattat
    19081 gataatccac actctaacag tttgttggtt tttctagtat ataacactca tattaaaata
    19141 aacactaaaa gccaaataac aatttaaata caaaatgtta tttggctaat ttagtttgat
    19201 actatttaac tatcttttac attcagttct ataatttcca acttttaatt tgtggtatta
    19261 tattcatctt aacagtttga ttctttaaag cttttctaaa tttctcagtt gaagaatctt
    19321 taaacatttt actagaaaca ctcttgaggt cttctataga ttcccaatgt tgtatcataa
    19381 tccacttttg agacttttca tcatataaaa gctcagtatc aataaaacca gattgaatag
    19441 aatgaaattt taattctaag tcatttacaa tttctataaa ctcttctttt gataaatttt
    19501 cttccacatt aaactcaact atttctgtaa taattttcat atgtaaaacc tccaatttat
    19561 tttcctatat catatcaaaa aataaattgg aggtattgta aaaaatcgac agtataatta
    19621 taaatagctg tactttatat tttctttaat cgtcttattc tcaccactaa atttaattgg
    19681 tgacgtccct gaaaattttt taaattcttt tataaagtgt gcttggtcat agtactcatt
    19741 ttcataaata aaatcaaaaa gttcatcatc tttataatta taaataagtt gattgcttgc
    19801 cattttaaat ctgtaaattc tctttaaaat cttaggtgta taccctgtat attttaaaca
    19861 gattctttcc aatgttttaa tatttatatt tgtatcatca caaaatgctt ttatattgct
    19921 atatctatta acatatataa atgaatttag aagctttaca tatttatttg aaattaaatc
    19981 ttcttctaat atggataata atacttcttc taaacataaa acaacttttt ctattgcttt
    20041 tgttggattt aatgactctt ctaatttttt tgcaaatagt tttgatactg cttctaggtc
    20101 aacaatttgt ctgttaaact ctgaaattgg tatctttaaa aatggataga gaccaaatgg
    20161 ataaaaagaa atccctataa cacgtatatt tccatgttga ataataaatc catgtttatc
    20221 ccttattcca ttaaaatgaa tattactagc aatatagtct tgattttcaa ttttatattc
    20281 cataacatca gaaagattga gaataatatc tatactgtct gtcggaagta acttattatt
    20341 taacagtata tttgattttg tctcaaaata ccaaataaac tttacccacg gctttaaacg
    20401 aacatttttg attgaatatc ttgtaactga taacattgtg aactaacctc tatactaaaa
    20461 ttttttaatt atgtactcca tcctaatatt atacagtata atttatgcta tcataaattt
    20521 tagttcactt tatatttatt ttatacattt tttcaatacc ttagaaatag gaagcgctat
    20581 tataagacct atcaatactt gcattaaatt acctggtatt gaagtaagag gtactataaa
    20641 attaccataa agtataactt ctgttacata atatccaaat agcatccaaa ttgatgaaac
    20701 acatattcct attacattga ttaaaaaatt attcccatta ttaccatttg cccatgcaat
    20761 ctttccaagt aaataaccca taactcctct aactataaag gtaaacggtg cccataaagt
    20821 ccacccagat atcaaatcaa aaagtgacat accaatagct cctgataaag cacctttttt
    20881 acttccaaat actattgctg atatgaacaa cattgcagtt cctaaatgca caagcccacc
    20941 atttatagat attggtaatc ttatgtttat aaatttagtt gcaataaata ctaatgctat
    21001 taatagtgag gtttccacca tgtctttcgt tgtaaatttt tgcttagctg taacttccat
    21061 actatatccc cctatctatt aaaaataata cttattaaat aaatttaaca aatttccatt
    21121 tcctttgaat cctaatgcac ttaaactttt atccaaaaca tttagtacat gttctatttt
    21181 atttagattt gcattctctc ccatatgacc tattcttaaa atagtatcca tatattgatt
    21241 tagtgatgtt gcaacaagtg tattataatt tttaagcata taatcagtaa gttctaaagc
    21301 tcctatactt tcaggaattt ttatagctgt gactgtatta gaatatcctt cttctaaaaa
    21361 tagttctaga ccatattcct caatagattt tctaacacta taagctatct tttcatgtct
    21421 atttaataca ttttctattc cttcttctaa tatattatca agtgctttat caagccccat
    21481 tatgtcactt attggcattg tataagggaa gtattttttt tcataatagc cttcccatat
    21541 atttaaatta caataaaaac ctattacatc agtttttcta ttttttatac aattttttgc
    21601 atcttgactt attccgacta tagtaagtcc tgctggtgca gaaatagctt tttgtgaacc
    21661 acctaatatt atatctattt tagattcatc tacacttaat ctttctccta ccatacctgc
    21721 tacagaatca actacagtta atattccata ttctttaagt aatggacata ttttgcttac
    21781 atcatttaat acgccagttg gagtatcaca atgcactact gttgcatatt taaaattact
    21841 atcctcatct aaaaatgctt tcaattcatc aatatctata cttttagtat attcacttga
    21901 aaaaagcaca tactccccac catacattgt tacaaaatct ttaaaacctt ctccatatat
    21961 accattgtca attacaagta ctctatctcc tttttctgta agtgatgcac atgctgcttc
    22021 aagacctaat attccttcac cactcaatat gtaaacatcg ttttttgttc caattatatt
    22081 accaaacttc tcgcatgtct ttttataaaa ttcaacaaaa tcaatatcta tatctggatt
    22141 tgttgttttt tctgctcttg ctaatcttac attttcccta gtttcagttg gtcctggtgc
    22201 ataattaatc ttcttattca tatatatcag ctcctattta ttttctatac gattattata
    22261 caaaattttc attttatatg gtacaatttt atactttttt agaattgtac cggtacattt
    22321 tctaacatga ataatatcag tatattagat atattattga ttacataata aaatacagag
    22381 caaacatctg cttaaattgt ttgctctgta ctatgcttta acctatttaa ttatgaatct
    22441 ctttaactat atttttttat atgttttttg ctagttaaac taaccataaa tatttgtatt
    22501 ttattcactg tatcttactc tatcaataag agattcttta ttaaaacttc gtcctatcaa
    22561 ataagttgtt ataaattgag ctataagaat acacacaatc atcaacaaga ttggtactat
    22621 tggcaaagaa tatgttgcat aagataatcc agtctttttt agaaatataa cagcaatata
    22681 ccctaatata cctccaaata gaatacttcc tatcatcatt ccagaagtgt aatacattgc
    22741 ctctgaatta agcattttca ctaattgttt attagtaaga ccaatagctt gaagcatacc
    22801 aagttctttt tttctagtaa ctatgctagt tatcattgtg ttgacaagat tcataaatcc
    22861 aatcacacct attatgcctg ttagcgtgta tcccattatc tttataaatg aaatagtagc
    22921 cttatttgac tcaattctac tatctataaa atcagtctct aaaaatccat tatttttagc
    22981 aatcttcttg atactatctt tgatttcttg atactttcct tcttctgtat atattcccaa
    23041 gctactagtt gaatcgtatt tcaaagttct atcaataaaa tccttcccta taccaaaact
    23101 tggaaccccc atagctattg cttgaacttt aaattctttt tttatctttt tatctccatc
    23161 atataaagtt aatcttattt tatctcctgc ttttactccc atttcttttg ctgtatcaac
    23221 atgagtaatt actatttcat ctccagtttg aagtctttca agatttattt ttccatctat
    23281 tatattcttg tttaatgatt ttacatcatt ttcatttata ctttgtaaaa ggtcatactt
    23341 aaattcaaca tcatagtcct ccataccaat ttttacactt cttgcagatt ctattctttt
    23401 aattccttcc atgttcttta tttgattaaa aaaatcttta cttagaggat tatccatttg
    23461 taaaatattc aaattatctt taggattttt atcactgttc atttcataac cagatagtct
    23521 aacttctata tcataaggaa agtgttctct tgccattttc tcagcatcaa aactttctaa
    23581 ggcagttgat actgttataa atatagttcc actaagtata agtgatgcca atgtcatgta
    23641 agtacgtttt ttatttcttt ctaaattggc atgtgataat ctctttagat ttatattttt
    23701 atatcctttt ctcttactct tattacttat tttatttcct gtatatctta ctgcgtcaac
    23761 aatggatact tttgatgcta ctttcattgg ttttaaaagt gataaaacaa cagatataaa
    23821 gcttattacc acaaccgata atataactgg taatcgtgat gattttacat ctatatccat
    23881 aaatgatttt attataatat ttgcaagtac atagcctaaa attattccta ttggtattgc
    23941 tattccggct aatatgaacc cttccttaaa tactatattt tttacttgtt ttttagttgc
    24001 accaattgct ctaagctttc caaattcttg aacctttgta actattgaaa tataaaatat
    24061 attgtatatt actaaaatac tagacaatac aattactaac cctacaaata ctccacccat
    24121 aattgtttcc atatctggtt ttaaagcatt tatatagttt tcatttacag ctacattaat
    24181 agtgtccaat cctacatcat tagcaatctt atttaatttt gattttgctt gttctatcga
    24241 taaattactt ttatcattta atttcacata tatattaaaa tcttcttgtg acatatctct
    24301 agtatttctc atatatgatt ctgatataat agcataataa tatttacctg cttcagatat
    24361 ttcgctagtt tttaagattc cacttataat gaactctttt tcaatttttt tatcactttt
    24421 atagtcatta taagatattt ttatcttttc acctaaatct ttatttttat aaccaagtgc
    24481 ctttaaagct ccactatcta acacaatttc attctctttt gatggcaatt ttcctttgat
    24541 aaattcaaaa ctatttaatt tcaaagcatc ttcattcatg tatgctagag aaatatttga
    24601 atcatcttcc agtttttcaa cacctactcc attcatttta ccagttaatt caacatctgc
    24661 atgattactc aatatatcag cttgtttttc gtctacattt gctaatatac cttgataatt
    24721 acctgaatac tttatagtat tttctttttg atatgctcct gcatcaacaa tataaattcc
    24781 aatagaagaa agcattaagg ttgaaagtat tatagtaatt attattagaa tactttttga
    24841 tttattttgc tttaaattac tttttgctat tttagtagta ttaatcattt ttatcaccaa
    24901 cctttgacac cctgccatct tctatgaata taactctatc agccatttgg gctatactgt
    24961 catcatgagt tatcataact agagtttgac tgtacttctt agacatagat tttaatatac
    25021 tcataacctc atcagatgtt ttagaatcta ggtttccagt tggttcatca gcaagtatta
    25081 tagctggtct tgtagctaat gctcttgcta ttgccaccct ttgttgttga ccacctgata
    25141 atgtatttgg taaaacgtcg tgtttatttt caagtcctaa tgattttaaa agctctttta
    25201 taaaaggttc atctacctct ctaccatcta aaccgattgg caacactaca ttttcccata
    25261 cattaagaga tggaattaaa ttatagcttt gaaatataaa ccctattttt cttcttctaa
    25321 atacagcaag ttcttcttct tttaatgtaa atatattttt attatctata tacaccttcc
    25381 cagatgtagg tatatcaaga cctccaatca tatgcaaaag tgtacttttt ccagaacctg
    25441 attttcctat tattgcaaca aattcacctt catttatttc taagtcaaca ttgtccaaag
    25501 ctcttactaa gctttctcct tttccataat gctttgataa attttcagta tatagtattt
    25561 tcataaaatt atcctctcat tctaagttat attactccat attttattaa ttttttattt
    25621 ctggagctgt ttatacttat ataataaatg tagaggctta cattttcctt tcatttaata
    25681 ttacatagtt gtaagaaaca aagaaaactt gctccctatt cctacttttg aagaaactat
    25741 aatatttcca ccttgttctt ctaaaatctt tctacttaaa tacaatccta ccccactacc
    25801 ttctacagat tcaatttcat cagcagaacc tctataaaaa cgtttaaata tattattaaa
    25861 ctcacttttt tcaatcccta tgccattatc ctctattata attttaaaga aattagcagt
    25921 ttcttctaca gatatattta tttctccatt tacctgtgta tactttacag cattgtctaa
    25981 tacattaaaa atactctcct cagtccactt tttatcatgt ggtataattc tactgttaaa
    26041 ttcatttaaa tttaatgtta tacttttgct cttagcctta ggggtaacac tttttactgc
    26101 atttagaata gtatttttta tatcattatc ttcttttttt atgtttatca tgctaatttc
    26161 taaccttgat atgttcacta aagaatttat taaattttct aatttattta tagaagcctt
    26221 attggttaat aagaactcgt tggcttcatc ttcttctaat tcttcttcta taagtaatgt
    26281 attgtataat tttaaggatg ctaaaggagt ttttaattga tgggaaatat ctgttactaa
    26341 tgattttaca ctttcttttt ctttttctaa tttaataaac tttatcttta aacttctatt
    26401 aagctcatat agacagttat ttatcttact aaatatgcct tctttaaagt attcattata
    26461 ttctactaga taaccagttt cttctgactc agtagtgtcc ttcaagtagt tttcttgtga
    26521 catcttatct actataaaat aaattttact caatctttta tttatgtatc ttataaaata
    26581 atatattata cctataacta ctataaacat cattaaaaaa ataaaaaatg attccttaaa
    26641 aaaagatttt aagtataagc taaaattttt atcatttgac attttatttt ccaatccata
    26701 gccaaatttc ttaaatgcct tttctccagt agacacctta ctataatcag aattataaat
    26761 agaatttact atatctattt catcattagg atatttttca atcaatattc cagcaacatt
    26821 ttgagtattt tttatttgat gttcatacaa tttatgtaat ctgctatatt caaaaagtat
    26881 aaatacaagg gttactatta gtacagataa aacagaatat acaataactc ttctcattat
    26941 ataatcatct ttcacactta aaccatcttt gaggttcata ttttttagca tttttttata
    27001 ctcctttctc cccacatata tccaacccct cttactgttt ttatatattt aggttcagat
    27061 ggattttttt caaccttttg tctaagtctt cttatattta ctgcaatcgt attatcatct
    27121 acaaaattac cactactatc ccataaagat tctaacagtt gctctttagt caatgtctgc
    27181 atagaatttt ccatcaaata ttttaatagt tttgtctctg tcttacttaa aattatctct
    27241 tcttccttat catctgttct tataattaat ttattttcta tataataaaa gaataaatca
    27301 tcacaaacta atagagtata attttttact gtatttactc ttttcataag cgcattcact
    27361 tttgatatta ataccatcaa gctaaaaggc tttgtgatat aatcatctgc acctaaatca
    27421 tatcctgtga caatatccac ctcttcatca agagctgtca acattattat gtaaacatca
    27481 cttttctttc taatctcctc acaaaagtca aaaccactac catcaggtag acctatatcg
    27541 gaaataatta agcatatctc ttcttttgcg aaaatatttt ttgcttcttc tataccaaat
    27601 gcactaaaaa cattgtatcc ttcttttttt aatttaaaac taattcctct atttaaagat
    27661 ttgtcatctt ccaaaagtaa tatattattc atccctatat cctccattat catacatact
    27721 agctaaatta tatcatacta ataaaatcat ttttttatca atttgtaaat atatcagaag
    27781 ttgttaatat gatttcattt acttgaataa aaactaaaaa tccccataat actatgccat
    27841 ttagtataat tatttttcat aaaatacaat tttatttaaa agtaatcatg ttaaaacata
    27901 aaacaaagga aattagtatt tatataacta atttcctttg ctgtttacta ttatctaaaa
    27961 ttaaattact atctactaat ttttgctcat taatttttat tttgactttt tatcctttaa
    28021 ctctttttga ttaaaattca ataaacatat caatgtactt gaaactataa atataattat
    28081 actatttgaa aaacctaaat ctcctgtttt aggagatttt tctttagctt ctcttttaga
    28141 tattcctaga ttagaacttt ctctagaact tgttttatct aagcttttat ttttatcact
    28201 atcagagctt tttatattat tagatagatt ctcttcatta tcattgatat ttttttcatt
    28261 ttcacttaat atcaaatcac tctcatgatt cttgcttatt aactcatctt tagatttaat
    28321 accaattctt ccatctacat tttttgatat ttcttttttg tactctatat attcaatgac
    28381 tgcactctct aaatttccaa attcctttag tactggctct tttgaaaaac aaggaaattc
    28441 atctccacct gatgctatat aatcattcgt tgcaactaca tactttttat ttaaatctaa
    28501 tggctttcct tctttttgaa tatttgttat tttttcgcct tctttttgtt taggattaaa
    28561 gtaatatgaa attcctccca cttgcaaaaa ggctgaactt ttttctggat aaagttttac
    28621 tccatgctcc aatacatctt taatctgtgc tcctgtaagt tctttggtaa ctatagtatt
    28681 gctaaaagga aatacatcta caatatctct tcttgttata ttgccttttt ctatcgtatc
    28741 tcgaatattc cctccattaa ataaagaaat atcagcctta gttttatcta gtaaaatgtc
    28801 acttattaaa ttacccaaat tagtttcttt tgtccttaca ttttcatatg aaccatctag
    28861 agtatttgta gttaccccta taactttatc taatattttt tcttgttgtt cttttatttt
    28921 atcaaccttt ttacttacaa cggagtcaag cttatacttt aatgcttctt cttttccaat
    28981 taatctagct gatttatttt ctatttttat atcttctaca tcattagtat ccaaagttag
    29041 ttttatctcg ccaatatttt ctaaatattg tcctgcactt acaatcaatg tatcacccac
    29101 ttgtttacca ttttcaaact tagtgtggct gtgtccatca ataatcaaat caattccttg
    29161 aacctcattt gctatatcta tactatttgg cttacttaaa ctatttgtac caatatgaca
    29221 tattgcaact attacattgg caccctttcc ttttaattca tttacttgtt ctgttgcact
    29281 ttctatagga tttttaaact ctaatccttc cacattttta aatccagttt tgctctttgt
    29341 ttcctgggtt gagagtccaa aaaatcctat ttttattcca tctatttctt ttatatcatt
    29401 actatcaaaa acttttttcc cattttcaaa aatatttgat gataaaaatt ttaaagtatt
    29461 ctctcctaaa ttagcacttt tagataactc aaacagtcta tcttttccat aattaaagtc
    29521 atgatttcca ggagtaatat agtcatatcc agcacattta attaattcaa ttgcactttt
    29581 ccctctatcc atatttacta ttggaagtcc atgcaaacaa tctcctgcat ctactaaaat
    29641 tgagtttgga gtttcttttt tcaatgtggc tagatttcct atttgtatat ggtcatcacc
    29701 ttcagcatat cttccatgta tatcattagt gtgaaaaatg gttatttctt caaatcgctc
    29761 tagcccataa gaaataaaac tctgacttgt tgtaattaga attataatta ccaaaattaa
    29821 atacc
//
LOCUS       DAYASV010000035        29299 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYASV010000035 DAYASV010000000
VERSION     DAYASV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.035"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            99..815
                     /locus_tag="ACGI68_001576"
     CDS             99..815
                     /locus_tag="ACGI68_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HFH0138019.1"
                     /translation="MKLDLFLLAIIPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            894..2204
                     /locus_tag="ACGI68_001577"
     CDS             894..2204
                     /locus_tag="ACGI68_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFH0138020.1"
                     /translation="MTELPKEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            2304..2840
                     /gene="lepB"
                     /locus_tag="ACGI68_001578"
     CDS             2304..2840
                     /gene="lepB"
                     /locus_tag="ACGI68_001578"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0138021.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            3006..3542
                     /gene="lepB"
                     /locus_tag="ACGI68_001579"
     CDS             3006..3542
                     /gene="lepB"
                     /locus_tag="ACGI68_001579"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0138022.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            3552..4307
                     /locus_tag="ACGI68_001580"
     CDS             3552..4307
                     /locus_tag="ACGI68_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFH0138023.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EILTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            4381..6048
                     /locus_tag="ACGI68_001581"
     CDS             4381..6048
                     /locus_tag="ACGI68_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HFH0138024.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            7007..7936
                     /gene="glsA"
                     /locus_tag="ACGI68_001582"
     CDS             7007..7936
                     /gene="glsA"
                     /locus_tag="ACGI68_001582"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFH0138025.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            8034..8597
                     /locus_tag="ACGI68_001583"
     CDS             8034..8597
                     /locus_tag="ACGI68_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138026.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            9085..9921
                     /locus_tag="ACGI68_001584"
     CDS             9085..9921
                     /locus_tag="ACGI68_001584"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008833 - deoxyribonuclease IV
                     (phage-T4-induced) activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFH0138027.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            10205..11200
                     /locus_tag="ACGI68_001585"
     CDS             10205..11200
                     /locus_tag="ACGI68_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0138028.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKEYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(11457..11861)
                     /locus_tag="ACGI68_001586"
     CDS             complement(11457..11861)
                     /locus_tag="ACGI68_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFH0138029.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            12883..13797
                     /gene="queG"
                     /locus_tag="ACGI68_001587"
     CDS             12883..13797
                     /gene="queG"
                     /locus_tag="ACGI68_001587"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFH0138030.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKDRQQKGYLTG
                     MEEPIIENRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLNYDEIQNISNKEFKR
                     KYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            13903..16140
                     /locus_tag="ACGI68_001588"
     CDS             13903..16140
                     /locus_tag="ACGI68_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0138031.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            16338..17507
                     /gene="nth"
                     /locus_tag="ACGI68_001589"
     CDS             16338..17507
                     /gene="nth"
                     /locus_tag="ACGI68_001589"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFH0138032.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKEEINKKGMNKKDIIEKEEINKKGMNKKDIIEKEEINE
                     KGIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKGETEKKTKKSSSNKATQ
                     KTSKETKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAFELLIATILSAQC
                     TDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKSKSQKIKDTSEQLCELY
                     DGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSNRIGIVDEPNPQK
                     TEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCNYYKELNETK"
     gene            17701..18171
                     /gene="trmL"
                     /locus_tag="ACGI68_001590"
     CDS             17701..18171
                     /gene="trmL"
                     /locus_tag="ACGI68_001590"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFH0138033.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            18207..19055
                     /locus_tag="ACGI68_001591"
     CDS             18207..19055
                     /locus_tag="ACGI68_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0138034.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            19343..20347
                     /locus_tag="ACGI68_001592"
     CDS             19343..20347
                     /locus_tag="ACGI68_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0138035.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            20473..21585
                     /locus_tag="ACGI68_001593"
     CDS             20473..21585
                     /locus_tag="ACGI68_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0138036.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLLTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDFIIAVGARFAEPIKEAATKYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            22075..22836
                     /locus_tag="ACGI68_001594"
     CDS             22075..22836
                     /locus_tag="ACGI68_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6873 family GME fold protein"
                     /protein_id="HFH0138037.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            22839..23657
                     /locus_tag="ACGI68_001595"
     CDS             22839..23657
                     /locus_tag="ACGI68_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HFH0138038.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            23837..24439
                     /locus_tag="ACGI68_001596"
     CDS             23837..24439
                     /locus_tag="ACGI68_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HFH0138039.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    25051..25289
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            25353..27272
                     /gene="thrS"
                     /locus_tag="ACGI68_001597"
     CDS             25353..27272
                     /gene="thrS"
                     /locus_tag="ACGI68_001597"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFH0138040.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            28040..29131
                     /locus_tag="ACGI68_001598"
     CDS             28040..29131
                     /locus_tag="ACGI68_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0138041.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT        11837 a         2672 c         5433 g         9357 t
ORIGIN      
        1 atgtatattt tagtatgtat ttaaaacacg aataagttaa aaataagtag atgatataaa
       61 tcataaataa atcaaaaatt ttgaaaggaa aataatctat gaagctagat ttatttttat
      121 tagcaataat tcctatattg atagggatgt tttggataag gtctaaagat agatattgta
      181 gagaacccct aatccatcta attaaatttt ttttaatagg tgcttttcta agtgttataa
      241 ttatactttt agaaaattta ctgatgaagt ttaatgtatt tgaagggtat tcagaactaa
      301 tatatgtatc atttgtagtt gcaggacttg tagaagaagg tgtaaaagct ctgatactga
      361 ttcctgccct aattaaagaa aaacacttta ctgaaaagtt agatggtatt atttactctg
      421 tatttttagc tttaggattt gcaacaattg aaaatatggt ttatattttt tctgaaagta
      481 gaaatctagc actgcaagtt ggtattaata gggcagttat ctctatacca gctcatgtaa
      541 tgtttgcaat tactatgggc tactatattt ctaagtataa atttgaggga aataaaaata
      601 aaagacgtga gtatttgttt atggcggtgt taattccaat tttattacat ggagtttttg
      661 atttcatatt gatgatagaa tatagatggg ctattatatt acttatagtt tatgtaataa
      721 ttttgtggaa gataaatctt gataaacttg agaaatatat gaatcactct aaaaaagtgt
      781 ttttcggtaa tcttaggaaa aagaagaaga aataaaattt aaaggaaaga cataaataat
      841 taaagtataa taaatgacac acaagtaaag cagattgaag ggggattata gatttgacag
      901 aattaccaaa ggaattttta gaagatattg aagaaatact aaaagatgaa tatgatgatt
      961 ttataaaaag ttatgaggaa agtaagacta ctgggcttag aataaatact ttaaaaattg
     1021 acaagaaaaa attatttgat ttggagttgt ttgatttgaa tcaaatacca tgggcagaag
     1081 aaggttttta ttatgatgat aaaattgaca gacctggtag aaatccactg catgaggctg
     1141 gagcatacta tttgcaagag ccttctgcaa tgagtgtaat accaaaggca gatattaaag
     1201 agggtgaaaa agttctcgat atgtgtgctg ctcctggtgg aaaatctaca tatatactat
     1261 ctaagctaaa cgatacaggt ttattggttt ctaatgaaat taatgctagt agaattaagg
     1321 ctctagggga aaatttagag cgatttggag cgagaaattg tattataaca aacaccgatt
     1381 ctaatagctt aagaaagacg tttaatgggt attttgataa ggttattata gatgcacctt
     1441 gttctgggca aggtatgttt aggaaggatg aggttgccat atcagattgg agttatgcaa
     1501 aggtagtaga atgtcaatcc atccaaaaag agattataag agatggatat aaaatgttaa
     1561 agaaggatgg cattttaatt tactcaactt gtactttttc aagggaggaa aatgagcatg
     1621 ttataaatga gtttatcagt gaatatccac atgcagaact tattgaaatg gaaagattat
     1681 ggcctcataa gataaaggga gaaggtcatt ttgttgccaa aataaaaaaa ttagaagaag
     1741 aagaatgtaa tgtcaaagaa ttaaaaataa aaaatttaga taaagaaata aaggattata
     1801 gggagtttga aaagaaattt ttaaaggttg atttttatag taataaaaaa aataaatttt
     1861 atttaagagg agaaaatttg tacctacttc ctgaaatata tccagatacg aaaaaactaa
     1921 aagtattgag atatggtttg catttagggg ttttaaagaa gaatagattt gagccttctc
     1981 atgcactatc tcattactta aaagttgaag atgtaaaaaa tgttgaaaac ttcagtgtac
     2041 agagtgaatc aattcttaaa tatttaaaag gggatgttgt aaattctaat gatagtagag
     2101 gatgggtttt ggtttcggtt gaaggaatac ctttaggttg gggaaaagaa tcttctggtg
     2161 taattaagaa tcattatcct aaaggtctta gaaaagtatt ttaaactatt agaaaaaaga
     2221 agtataatat ttatattgta ggtttatggt agattatttt gctgtagatt gacttaatat
     2281 ttaacacaga ggagacgatt ttagtgggtg aagcagttaa aaaagaagtt gtagaatgga
     2341 taaaagtgat tgtcatagct cttgttttgg catttgcaat aactcgtttt atagtgccaa
     2401 caatagtcaa aggagaatca atgtatccta cattagttga acgtgattat ttgatagtta
     2461 acagaattgc gtacaaggta ggagagccaa aatacaaaga tataatagta ttcaaaaccg
     2521 acttaacaga ggaaaatgga aagaaaaaag atttagtaaa aagagttatc ggggttcctg
     2581 gtgaccatgt aaaaatacaa gactccaagg tatatgtaaa tgataagttg ttagatgaga
     2641 cttcctatat acataataat cgtactgatg gagatattga tatcgtagtt ccagaaggaa
     2701 aattatttgc aatgggagat aatagagaaa aaagtttaga tagtagatac gatgaggttg
     2761 gattggtcga cgagcatacc attttaggaa aggttctagt cagattgtat ccattttcta
     2821 agataggaac tattgactaa acatataaaa tttggagcaa tcgctgatta gctgaaatca
     2881 ccagaccata cacaatagtt gtctatataa aaaagacaac accttattta gtagacttat
     2941 ctaaaagata gtgtttaaga gacattatca ataaagataa tataactata aaggagaatt
     3001 tgtgaatgaa tgaaactatt aaagaagaga ttgtagagtg gataaaaata attattactg
     3061 cacttttttt tgcatttatt ataactcgtt ttataaaacc aacattagta aatggagaat
     3121 caatgtaccc aacacttaaa tcacatgatt atttggtagc aaacaggatg acatataagt
     3181 tatcagaacc aaaatgtgga gatataatga tatttaagac tgatttatta caagagaatg
     3241 gaaggaaaaa agagcttgta aaaagggtta taggtgttcc tggtgaccat ctaaaaatta
     3301 aggattctaa ggtttatata aatggtaagt tattaaatga agtttcatat atacatgata
     3361 attatactga aggcgatatt gatatggtga ttcctaaggg aaaagtattt gcgatgggag
     3421 acaatagaga agttagttta gacagtagat ataaagaagt gggattagta gatgaagaaa
     3481 atattaaagg aaaagttatt ttaagagtat ttccttttac agatataggt atttttgagt
     3541 aggtgatttg tatggcagtt ggtatagcgt ccttattgtt taatattgag gaggctttaa
     3601 acatttgtga gagtataaag caaataactc atttagagat tggaattgac aatatatctg
     3661 aatgtagtga gttatgtaaa tataaagaga gaatttctaa gttaggatta tctataggga
     3721 tacatttgcc tatggaatta aatacttgcg aaaacataga gtatatacga aattcatgga
     3781 taaattttat tgagaagata gaatttgaac taaaaggatt tgatttaagg tattttaacc
     3841 ttcatttggg atatgttatg acaaatagag ttagaaagaa tagagacaaa tatttaggaa
     3901 atagtgtgga ttttttagat aaattaaata caaattcata tgtatgtata gagaatactt
     3961 attcaaaagg tggggatttt tcaaatattg gaaatatatc ttatgatttt gaatatatat
     4021 ttaagaggat taaaaattct aagatttgtt tttgttacga tactggacac tgtttaattg
     4081 atgaggatgc ttatgtaaag aacttaaagg ataaaataag actgatacac ttaagtgata
     4141 atgatggtat caatgataca catgttggta taggaagagg aattttatct gaagagggga
     4201 ttaaagagat cttaacatta gatgcagaat atttagtatt agaaataaat tacgaagaca
     4261 ttgaagatac tataagtaaa ttgaataata ttgtaggaga gggttaaata cctctcttta
     4321 tcttgttgat atgcctcatt aattaaaatt tatattcatc aaaaggaagg agatgttgaa
     4381 gtgaaaaaaa taaacttaaa aatagaagga aaagataagg aatattcatt agaagaaaat
     4441 tcccctggaa tcagattggg agatatagca aaagaatttt gtgatgagca taagggttat
     4501 ataacacttg cagttgtaga caataaacta aaagagctaa attgtagagt taagaaggat
     4561 tgtgagataa attttttaga tactactaat gaagatggag agagagttta ctttagagtc
     4621 atgtcattta tatttgtaat ggcatgtaga gaaatctttt gggatagcag agttacaata
     4681 gaacattcac tatcagatgg gctatattgt gaagttcata tagatagaaa gcttaaagaa
     4741 gcagatgttg aaaatataaa aaacaagatg aaagagatag taaacaacga ttatgttata
     4801 gaaaaaattg aagttacaag aaatgaagct atttctatct ttgaaaataa tgaaatgtat
     4861 gaaaaggcag agcttttgaa atacaaagag tatgatagtg ctaaagtata taagtgtaga
     4921 aactatatag accattttta tggttatatg ttgccatcaa ctggttatat aaaatcattt
     4981 gatattaagc tttataatgg aggcgttata attttaggac ctagtgaaga agataagact
     5041 cttcctatga aatttgtacc tcaacctaag ctatcaagtg tatattatga agcagaagaa
     5101 tggtcaagac ttatgggaat tgacaaggta gtatctctta ataaaataat agaaaacaat
     5161 gaatatggag atataataag gacatttgaa gcattgcatg aaaagaagtt atcccaaata
     5221 gcggatatga taaaggatgg caataagaga gtagttttaa tagcagctcc atcttcatct
     5281 ggaaaaacaa gttttgctca cagattatca atacatctta gagtaaataa tttaaatcct
     5341 atttcaattt ctttagatga ttattttata gatagaaaac atactccttt agatgaatat
     5401 ggaaattatg attttgaatc aatttatgca atagatttag agaagtttaa cttagactta
     5461 aagaaacttt tagctggtga agaaattgat gatataagat tcaattttaa agaaggtaag
     5521 agagaataca ctggtaaaaa gataaagata gattcaaatc agccaattat attagaaggt
     5581 atacatgggc taaatcctat tttaacatca tctataccag atgaagataa atttaagata
     5641 tacataagtg cccttactca aataaatttg gatgaccata atcgaatccc tactgctgat
     5701 ttgagaatga taagaagaat tgttagagac tataatttta gaggatatag tgctgataat
     5761 actattttac aatgggcatc agttagaaga ggagaaaaga agaatatatt cccgtaccaa
     5821 gaggaggcag atgctatatt taactctgct tgtgtatatg aattggcagt tttaaaacga
     5881 tttgcgaaac ctttgttgga agagataaag gaagataatc ctgcacatat tgaagctact
     5941 agattactaa agtttttgca gtattttgtt gaattggacg atacttctga cataccaggt
     6001 atttcaattc ttagggaatt tattggagga agtaaaatag ttgattaatg tgtgtattat
     6061 agattgtttt atgaagatta aatggcatag caagtagtgt tattggttta tgttaagtat
     6121 attaagtgta aagtggagaa ttaaattgat acagatttta aagtttgagt tgatatataa
     6181 aaaggcttgt tttatacttt tgtatgaagc aagcttttta ttttaaattt tacttttttg
     6241 tttaatattt tgttaattta tgtgattatt ttttaaaaga gtaaaagagt aaaagagtaa
     6301 aagagtaaaa gagtaaaaga gtaaaagagt aaaagagtaa aaggtgttgg gttagaaatt
     6361 tttggtgtta taaaaattta aattactgga ttattagatt gctggattgc taaattacta
     6421 ggttgttaaa ttattgaatt attaggttgt tgaattacta aattactagg ttgttaagtt
     6481 actggattat ttagatagtt agattactag gttgttaaat tattgaatta ttaggttgtt
     6541 gaattactaa attactaggt tgttaagtta ctggattatt tagatagtta gattactagg
     6601 ttgttaaatt attgaattat tagattgttg aattactaaa ttactaggtt gttaagttac
     6661 tggattatta gatagttaga ttactaggtt gttaaattgt taaattattg aattgttaga
     6721 ttgttgaatt actaaattac taggttgtta agttactgga ttattagata gttagattat
     6781 tagattgcta aattattagg ttgttaaatt actggattat tagattacta aattgctagg
     6841 ttgttaaatt attaaattat taagctattt aaactattta aattgtttga aaaatgaatt
     6901 gattcttgta gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat
     6961 aaacttgtgc taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga
     7021 tgagacatta ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt
     7081 cgctagttac ataccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat
     7141 agatagcgaa aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag
     7201 tatttcaaaa ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt
     7261 ttcaaacgtt ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat
     7321 taatgacaca aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc
     7381 ccttataaat gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa
     7441 gttagcaaaa aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac
     7501 tggagataga aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa
     7561 tgtagaagat gttctagacc tttattttaa acaatgctca attgagatag attctgtaga
     7621 tttagcgaga attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca
     7681 tttaatgagt gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta
     7741 tgatgcttct ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg
     7801 tggtataatg gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga
     7861 caaaaaaggt aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa
     7921 attaaatatt ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata
     7981 tataattaaa ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag
     8041 aggtattggt attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag
     8101 atattctaaa agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg
     8161 aagaacctca tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag
     8221 aattagttga ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa
     8281 aaaatgtggt tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag
     8341 aagaattgta catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg
     8401 aagatgtttt agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg
     8461 aattagttga acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac
     8521 aacacaatgt tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg
     8581 aggaatgtcc tagttagaga attaattatg gattaattaa agatataata ttagagattg
     8641 aacatcaaaa tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt
     8701 ttaatctcta tttttttgtt tttttatacc tattgtattt gtaaataata tgtatagtgt
     8761 aagatgtata cactatctaa gaagtgctta gataatatac aaagtatgta agattataat
     8821 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
     8881 taaattaaat caatgctaga aatatgttaa aagaggaatg agattagttg ggatagaaca
     8941 tcccttagag cttacgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
     9001 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag
     9061 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
     9121 ggatttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
     9181 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
     9241 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
     9301 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
     9361 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
     9421 ggtggtggtg tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag
     9481 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
     9541 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
     9601 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
     9661 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
     9721 aatgatagta tgatgccatt tggagacaaa aaagacagac atgctcctat aggggaagga
     9781 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
     9841 tttttcttag aaactccttt tgatgatgaa ggtcacaaga gagagcttaa aatgataaaa
     9901 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatactcc accctcaaca
     9961 taattagggt ggagatacaa tcatgaaatg tgataattta aattaatgga taggaataac
    10021 gaaaattgta tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat
    10081 aaaatataat tatattatga atagtatgta aatacattgc aaagattctc ataatttgtt
    10141 gtataattaa aggtgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa
    10201 agatatgaga aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat
    10261 tccaatacaa aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca
    10321 aggaataaac tttattgaca gtgcaagagg gtatacaata agtgaggaag caatcggaat
    10381 tgccattgaa ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta
    10441 tgattcaatg aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga
    10501 tttatatcaa ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat
    10561 ggcttatagt gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac
    10621 tagtcacaat ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca
    10681 attcccttat aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa
    10741 aggaattggg ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact
    10801 ggctattaaa tatatactat ctaaagagta catagatgta gttataccag gtatggagag
    10861 tgttgaacag gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga
    10921 taataaggaa attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga
    10981 atattgtatg ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta
    11041 ttatactaga tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa
    11101 acctactgag tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat
    11161 aagagaaatg cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg
    11221 tgtattaaat ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat
    11281 ttacatagtt atagctatta tataaattta atatttgttt aatacttatt aaatatttac
    11341 ttaatgcttt ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat
    11401 tagtatagtt tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat
    11461 ttttcaaaaa tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc
    11521 ctagggttat ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt
    11581 tttattattc cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc
    11641 cccattctct tatttgggaa gaataatttc aagactgtta ttagattttc ttcgacagtt
    11701 tccccttgat actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta
    11761 gaatattttc cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta
    11821 gatttactaa taggtctacc tggaattgtt attttaatca aatttttcac catccttata
    11881 cagtatttat tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac
    11941 agagcttttt tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt
    12001 acaaatttta aaatttgata tatgatggac atattcatat tagtctatta taagaccttc
    12061 ataatattaa taagtatagt ttagtaaaga gagtaaaaag aaatccatat aagtcaagta
    12121 aagatttaat aaatggcaat gtaaatggga tattaattat aagaaaaaat caatcttttt
    12181 gcttaactat agaaagaatg tcaaaatata actaatgata tttgaagttt tagagtttat
    12241 attaaaaggt aagaaggtta agtgggtaaa aaaggttgaa tagattaaaa aggctaagta
    12301 tctaaaagag ttaagtaggt aaaaaagtta aataagtaaa tgaaaaaggc taagtatcta
    12361 aaagagttag tagttaaata agtaaatgaa aaaggctaag tatataaagg agttagtaga
    12421 taaaaaaatt aaatagataa aaagattaag tatctaaaag cgttagtaga taaaaaaatt
    12481 aaataaataa aaaagactaa gtattcaaaa gagttagtag gtaaaataat taaatagata
    12541 aaaagactaa ttatttaaag gagttaagta gataaaaaag ttaaataagt aaatgaaaaa
    12601 gacgaagtat ctaaaagagt tagtaaataa aaaagatgaa gtatctaaaa gagttagtag
    12661 gtaaaaaact aaatagataa aaagattaag tatctaaaag cgttagtagg taaaaaatta
    12721 aataggtaaa aagacgaagt atctaaaaga tttaagtagg taagaaaatt gaatagctaa
    12781 aaagattaag tatttaaaag agttagtagc tagaaaaggt taaaaagact aaaaaaatta
    12841 aataaattta aaaagtcaat tagattaaaa aggaataatt ttatggataa taaggaatta
    12901 aaagcatttt gtaatttaat aggattaaac tgtgtaggag tagcaggggt agataagtat
    12961 gataatttag aaaagatact taaggacaga caacaaaaag gttatttaac tggtatggaa
    13021 gaaccaataa ttgagaacag aattaaccca agacatataa tgaaggatgc taactctata
    13081 atagtctgtg catttccata tcatataaat agacaagaaa agaataaaaa ttcaaattta
    13141 tcaaaatatt gtcatggaaa ggattatcac attgtagtaa aggacttttt acaacaaatt
    13201 tgtgattata tttctaaaac agtaacagat tttaaataca agttatttgc tgataatgga
    13261 ccattagttg acaggtattt agcttattta tcaggaatag gatattttgg aataaataat
    13321 aatatcataa ctgatgaata tggttcttac gtatttatag ggtatatagt aaataattat
    13381 aaatttgaag aagataaacc actagaaaaa agttgtttta aatgtggaaa gtgtgtaaaa
    13441 tattgtccgg gaaatgctat attgggaaat tatgatatga accccaaaaa atgtttatca
    13501 tatataactc aaaaaaaagg agatttgtca gagaaagaaa aagttgtttt aaagaatggc
    13561 aaaaaggtat ttggatgcga tatttgtcaa gaagtatgtc cacataatgt tgaaatacca
    13621 acaactcata ttttggaatt taaagaaaat ataatagatt atttgaatta tgatgagata
    13681 caaaatattt caaataaaga atttaaaagg aaatatggag atagagcttt tagctggaga
    13741 ggaagaaata taataaagag aaatatggaa ataattttag ataagtatga tgactaataa
    13801 taagagtctg tttcatatac tttaatgtgt atttacttta acacttgttg tgtaaaagtc
    13861 atattatatc attaaagtta aatatgggga ggtgggggaa tattgaaata taataataca
    13921 tctgaaataa atccactaga ttatatattg ggtcaagaaa gagctgtaga agctatggaa
    13981 ttagggttga aaataaataa tccagcgtac aacatatata tagctggtga acctggaact
    14041 ggtaaaagta cttatgcctt aaaggtttta aatgagtatg catccaaaaa aaatacgcat
    14101 aaagattggt gttatatata taattttgaa aaccctagag agcctattat agttgaatta
    14161 gaaaaggggt ttggaagaga acttaagaaa gatatggaga aattaattga aagtctttta
    14221 gaagatttta aaaatgcgtt tgaaagtgaa aattttgaaa tagaaaaaaa taaacttctg
    14281 gatgaatatg aaatagaaaa agatatgctt ttgaagcaaa ttaaaaaata tggagaagaa
    14341 aaaggattta aattaaaaca gagtaaaatg ggaatagttt ttatcccttt gaaagaagag
    14401 gaagatgaaa gtgatgagtc tgatgaagag ttttacaaag ctaaaagaga actagaaaat
    14461 atggcgatac aggtcgtata taaaattaga aacttagaag aaatagctga aaaagcagtg
    14521 ttagagcttg aagaagagat agctaaactt gtagtagaac ctcatatcaa gagattgtgt
    14581 gaaaaatatg aaaattatga taagattcaa acttatctgg aaaatataaa gaaagatata
    14641 atagaatata tgtatttatt ctatttagat gaagaggagt taaaagataa gtatgataaa
    14701 gagcatttta ttaaatataa aattaaccta tttgtagaca atggaagctc aaagaataaa
    14761 gaatcagcac ctgttgtagt agagataaac ccaagtccag caaatctatt tggaaaggca
    14821 gagtatgatt atgcaaatgg aaatataaag actgatttca caaaactatt atcaggagca
    14881 tttcataggg ctaatggtgg ttatttagta ctttatgcag accaattact aaaatataca
    14941 atgtcttggg aaattcttaa aaagactttg cagactaaaa aagtaatatt ggaaacacaa
    15001 acagcaatta aaccagaaaa tatgccattg gatgttaaat tagtactgat tggaagccac
    15061 tatatatatg atattttata tagatatgat gaagattttg aaaaatattt taaagtattt
    15121 gtagactttg atagtgaaat ggataaaaat gaagataatg aagaaggaat agcaagattt
    15181 atagcatacc aatgtgataa gaataattta aggcatttta ctaaatcagc agttgaagag
    15241 gttattaaat ttagcactag attatctggt gatttagaaa aattatcaac taagtttaat
    15301 aaaataatgg aagttgtaat agaaggaagt gcttatgcag agtttagaaa ttcggagtat
    15361 acagagcagt gtgatgttaa aaaagcaata agtgaaaaga gaaaaagaat caatagagtg
    15421 gaaacacata tggatgaatc aattgaaaat ggatttactc taatagaaac agagggaaga
    15481 aaagtaggag tgataaatgg tttgtcagtc ttaagtacag gagaatactc ttttggtaga
    15541 gcttcaagga ttacagcgac cactagccca ggaagtaaag gcatagtaaa tatagaaaga
    15601 gaagtaaata tgagtggctc tatacataac aaaggagtac ttatattagg tggctatctt
    15661 tctgagaatt ttgcacagga tttactattg tcactaaatg catatgtttg ttttgaacaa
    15721 aattatggtg gaatagatgg agatagtgca tcttctgcag aattatatgt actattatca
    15781 tctcttagtg gagtatcaat taaacaaaat attgctgtta caggttctat aaatcaaaag
    15841 ggtgatatac aagtagttgg tggtataagt gaaaagatag aaggatttta ttctatatgt
    15901 aaaactaaag ggttaaatgg tgagcaaggc gtcataattc ctagaaaaaa tcaaagaaat
    15961 ctcgttttat cagatgaagt taatgatgca gtgcgagatg gaaaatttaa gatatatact
    16021 gttgaaagag tagaagaggc tatagagata ttgacagatg ttaaatttga agaaataaag
    16081 aaattggtaa aagaaaaatt aatatctttc agcaagatac aaactgtgtc aaaagaataa
    16141 gtttattata gaatttaatg ggtatttatg tataataaaa agcaagaact ttaataggtt
    16201 tttgcttttt ttgtaaatat atataaatta aattcttatt ttgtatgttt tatatattga
    16261 tgtttatttt tttgttgata tgattaaact atttataaat agcgttttat aaattggtat
    16321 tttgaagggg gataattatg aaaaatgtaa atctaaaaga gataaatgaa aaagatgtaa
    16381 ttttggaaga agagataaat aaaaaaggaa tggataagaa aaatataatt gagaaggaag
    16441 agataaataa aaaaggaata gataagaaag atataattga gaaggaagag ataaataaaa
    16501 aaggaataga taagaaagat ataattgaga aggaagagat aaataaaaaa ggaatgaata
    16561 agaaagatat aattgagaag gaagagataa ataaaaaagg aatgaataag aaagatataa
    16621 ttgagaagga agagataaat gaaaaaggaa tagataaaat gattggtaaa aaagatgaga
    16681 taattgatga gaaagatata gagaatttta tggatattga agaagataaa aaaagtatca
    16741 aagtaagtaa aaaaagtact aaaaaaggtg aaacggagaa aaaaacaaag aagagtagtt
    16801 ctaataaggc tacacaaaaa acaagtaaag aaactaaaag taagtctaaa attaaaaatg
    16861 agtcagcaaa tgaaaaagac gtaaataaga ttttagacga gttagaaaaa ttgtatccag
    16921 atgctaaatg tgagttaaat tatgggacag cctttgaact tttaatagca actattttat
    16981 cagcacaatg tactgatgta agagtgaata aagttacttc agagttgttt aaaaagtaca
    17041 acacagctag agactttgct aatttgagca ttgaagaaat tagtaaagag ataaagagtt
    17101 gtggtcttta caaaagcaag tctcaaaaaa taaaggatac atctgaacag ctatgtgaat
    17161 tatatgatgg tgaagtacct gatagcttag aaaagcttat aaaattacct ggtgttggaa
    17221 gaaaaacggc aggggttgta cttagtaacg cttttaatca tccagcaata gctgtagata
    17281 ctcatgtatt tagagttagc aatagaatag gaatagtgga cgagccaaat cctcaaaaaa
    17341 cagaatttgc acttatggaa gctataccaa aagaaagatg gtcacactca catcatgtat
    17401 taatttttca tggaagaaga atgtgtaaag ctagaaatcc agaatgtgca agttgcccaa
    17461 taaaagaaga ttgtaattat tataaagaac ttaatgaaac gaaataactt ttagaaagta
    17521 agttgtacaa gagggatttt ctgatataat aatacttgtg aaaatgttag ataaaaaact
    17581 attatataca tgaaataaaa attattatta acattatttt atataatagt ataatattat
    17641 tgttattctg tttataaaat aaaatttgat aaataaaagc gatacttagg gaggattaaa
    17701 atgtcattaa atatagtatt agtagaacca gaaatacctc aaaatacagg aaatataata
    17761 agaacctgtg cagcaacagg ttcaacttta catttagtaa aaccactggg tttttcattg
    17821 gatgataaac acctaaaaag atgtgggctt gactattggg atatagctga tatacaatat
    17881 tacgatagct ttgaagaatt acaagaaaag tatccaaata gtaaatactt tttttctaca
    17941 acaaaggcta aacagtcaca ttcagatgtg aaatatgaag aaaattgttt tatagtattt
    18001 gggaaagaaa caaagggatt accagaacct ttgttgaaag caaatatgga tacttgtatt
    18061 agagtgccta tgttggatgt tgaaaaagct aggtcattaa atttatcaaa ttcagttgct
    18121 attgtagtgt acgaggcttt aagacaaata ggatacccaa atatgaggta gtttcaacaa
    18181 gcctaaatga gaaaggagca tacttaatga aaaatctaaa gataatctgt gacagtttat
    18241 cggacgtgcc aaaagatttg ttagataaat atgatataga tatagtttca ttaacagtta
    18301 tactggagga aagagaatac aaggatagaa ttgatatatc tggagatgaa ttttataaaa
    18361 ggcttagaga agaaaatgta tatccaaaaa cttctcaagc tacttatgcc caatttaagg
    18421 aaatttttga gaaatataca aatgaggata gagagatact ttatatagca gggtctgcca
    18481 cagctacagg tacgtatcaa agtgctgtaa tggcaaagaa tgatgtagat ggtaaaatat
    18541 atacatatga ctctaacttg ctttgtatgg gaactggttt acttgtaata aaagcaggag
    18601 aacttgcaag tgaaggaaag tctgttgctg aaatattgcc tgttttggat gaattaaaag
    18661 agaaggcttt tgttatattt tcagtagata cattggaata tttacaaaag ggaggtagaa
    18721 tatcatctac taaggcagca ataggaagta tattaaatat aaaaccgatt cttgaaataa
    18781 gagatggatt agtttcgcaa atatctcaag taagaggaaa gaagaatgta atttctaaga
    18841 tgatggaatt gataaaatca aattgtggtg atgatttatc agaccaaatt gtgtatatag
    18901 gatatagtga tgattttaag gaaaaagaac aattatctaa ggttatgcaa gatgaattaa
    18961 gacctaaaga aattagattt ttccaaattg gtgtgtgtat aggtgcacat gctggtcctg
    19021 gtgttactgg tatcatatgc ttaaaaaata aataatatat ttttggatga cattatttat
    19081 ttttttatta aattaggata gaatattaat tgttgagatg ttaaattgga tttattttgg
    19141 atgaaattaa gtgttgatat attagattta gtttgtaggg aagtgtgtaa atagataaga
    19201 atctattgca cactttttta tagtaagatt tattaaaaaa atttcaaaat gtacttgtaa
    19261 attgtatata aaaaaattat aaataggatg taaaagtaaa taggatataa aaatatcgta
    19321 aatgtatgag gagtgaaatt taatgcgttt caaaagaata ttgatattga tgttaactgt
    19381 agcaatgata gctggaatgt tggttgggtg tgctaataac ccaagtaata atcctagctc
    19441 tagcaaagat aataaaagtg atataagcga taaaagtaat tcaaaaaaat ctgtatcaat
    19501 gatactagat attgaaggaa caaataatga agctatgaat aattcagctt tattagcatt
    19561 aaacaatgct caaaagaaac ttaatataga tacaaacaaa gtggaatcag atgattcctc
    19621 aactttttct aatagtatag atattttatg taatgacaat tacgatttaa ttatagctgt
    19681 aggagctaga tttgcaaaac cattggagat ggtagccaag aaatatccaa agcaacaatt
    19741 tgcaattata gactatgagt atgacaagca accttctaat ataacttcaa tttcttatga
    19801 agataataaa tctggatacc tagctggatt aattgctgga aaaatgactg aaagcgataa
    19861 ggttggtttt ataggaggaa caaagagttc tagtagagat aaatttgaat caggttttag
    19921 agagggtgtt aaattttcta atagtagtat aaaggatatt tcagttgagt atgcagatgt
    19981 ttttaaagat agcaaatcag tagaatctat agctaaaaaa atgatggata atggagtgga
    20041 tattattttt tctactactg aagatgattc aaaagctgta atagatgcag taagggcaaa
    20101 aaataaaaaa gttatagcaa cgaataaaga ccaacatgag ttagctccag aaaatgttat
    20161 aagttctatt gtaaaagatt ttgagaatcc tacttataat ctgatacaaa gttttgtaaa
    20221 aggaaattat aaaggcggaa aggttataga aaacacagtt aagtctggaa gtactggact
    20281 tgcccaaaat agttctcaaa atattcctcc agatgtgtta gaatatgtta ataagaacaa
    20341 taaataatct actataatat ccaagaatac ttatgagttt agttatgagg tgaattggat
    20401 gttataatag acatatatag aaagacatat gcagaggtag gtcagaccta tctaaatcag
    20461 ggagtgaatt ttgtgaaaat aaaaaggata ttgatggctt gctttgtagt agtttttaca
    20521 atttttatat tagctatatg taattttgat tttgggtttg ataaagtaaa taagaataat
    20581 tctaatatag atagtgctaa atcgaaacag agtgagtcta aaagtttgct aacttcagca
    20641 gaaaaggata aatcaaatag taaaagtaag aataaagaag atgacacaga agatgattca
    20701 tatgtaagaa aaactattgg agtattgtta gataccgaaa acttaaataa cgaatcatat
    20761 aataacaatg tactatcagc cttaaaaagg gctgacgaag attttgatgt taggataaaa
    20821 cttgaaaaac caaagacttc taaaagtttt aaagagagta tagaaaaact gcataagtat
    20881 gaaccagatt ttataatagc agttggagcc agatttgcag agccaattaa agaagctgct
    20941 actaaatatc ccaaacagca atttgcaata gttgactata cttataaagt tcagccagct
    21001 aatgtaattt ctgtatcatt tcaagataat gttactggat atttagcagg acttattgca
    21061 ggtaaaatga ctgatactgg gaaagtcggt tttataggag gagttgaggg ttctagtaga
    21121 gataagtatg aatctggatt tagaaagggg cttaaaactt caaataaaga tgctgagtta
    21181 ctagttaagt attcaaatat atttgaaaac cctgaatcag ctagaactat tgcaaaggaa
    21241 atgagagatt ctgatagaga tgttgtattt tcagctacag aagatgatag taaaagagct
    21301 attcaggtag caagagaaaa tggtggtaaa gttatagcta ttaataaaga ccaatacgat
    21361 atggctccag ataatgtaat aagttcaatt ataaaagatg tagaacaacc tgtatatgaa
    21421 ctgataaaga acgttgcaaa agatggtttt aagggaagta aagtaatgga atttaaaata
    21481 aaagatgggg aacttggctt gacttcaaag agcagtagaa atataccacc agatgttttg
    21541 gaatacataa aaaaagagta taacaaagtg aaagatacat attaattatt gatgtagaag
    21601 attataaaaa tatattgctt aagtttagat tagcaataaa aagatatatt tattaaattt
    21661 gtgctaactg tacaaaatat attgattaag tttagattag tgataaaaag atatatttat
    21721 taaatttgtg ttaacagtat ggaatatact gcttaagttt agattggtga taaaaggata
    21781 tatttattaa atttagtcta actgtacaaa atatattgat tgagtttaga ttagcaataa
    21841 aaagatatat ttattaaatt tgtgctaact gtacggaata tactgcttaa gtttagatta
    21901 gtgataaaaa gatatattta ttaaatttga accaactgta caaagctata ctaattaagc
    21961 ttagaataac tatataaaat tataacaata agtgtgtggg cagaaaaatt ctgctacaca
    22021 ctttatttat attcacataa aaaatttatc aaaaaaaaga aaaaggagta aaaaatggaa
    22081 tttacaagag aaccatttat actagatgac gatttgtgtc tagctttggt ggacaaaaga
    22141 attagttatc taatggaaaa tgaattaaaa ttaagaaata tagagataat aaaaacaata
    22201 gaatgtaaag aattatatga aagcataaaa tatcacccgg atatatgtat ttgtaattta
    22261 ggaaaaggag atataattgt tgcgccgaat gtatataatc aatacaaaga gttattaagt
    22321 agatataaat ttaatataat taagggagaa gctaatttat taaatagata cccttatgat
    22381 ataccttaca atattgcgat agttggtgac tatgctatac acaattttaa gtatactgac
    22441 aaagcaatat tagattatat tgaaaataat aaacttaaaa agataaatat agagcagggc
    22501 tattctaaat gctcaatctg tatagtagat agaacgtcta taataacatc agataaaggt
    22561 atttggaaaa gtatgaagaa tacagaaata gagtgtttat tgatagaaaa aggtcacata
    22621 aatttatttg agatgaatta tggatttata ggtggatgta cagggttgat ttctaaggat
    22681 aaactagctt tttgtggaga tgttaaaaaa catcctgact atgaacgtat aaaaagtttt
    22741 gtagaaagta aaaacaagga gatagttaca cttagctgtg aaaatttact agatataggt
    22801 tctatagttc ctctcatgac aagaaaggag cgataacttt ggttgagaag gataatattt
    22861 cttccatgaa atttttgagg aataataatg taaacgatag attaatatat acgaatgaca
    22921 ttgtagaagt agtgatagat tctgcaaagc aagaagtgtt aaataaaaaa atacttaatg
    22981 gtactaaagc tgaacaaaga gctttaaata ttgatgagat atcaacggaa ataactagta
    23041 tgataataag tataatgaaa gagaaacttt tgaaagaata cattctaaaa tgctatgggc
    23101 gtacatatcc tgaagataaa aataatatat acatatattc actgaacata tttgctaaaa
    23161 aagagatttt tatgagagaa acagtattta ctagggtgag aaattatata gaagaaaatg
    23221 attttataaa tgtagaaggt tttattagat ttagaatgag agaatttatg aaatatattt
    23281 ctgctatagg tgatattgca gttgaggagt atttaataaa gaaagaccaa gatgaattta
    23341 taagagtttt aaaatacttt atagatactc aagaagaaaa gattgatttg cttaaagttc
    23401 atataatgga agataataca tttgttttat acgataaaaa tggaaataag atagacagta
    23461 tagatgatga agaaattata aatatggtaa taagagaaaa tttaaattat gaggactttt
    23521 taataagtac cttattaact ctatgtccca aaaaaataga aatattggat ttgttaaata
    23581 ataattgctc aaaggaaatt atagatactg tagaagcaat ttttgaaaat aaagttagta
    23641 taattatgga aaactagaat ctaagcatat aaaagctatc catataaaat taaaaaaatt
    23701 ctttttaaat cgacacaaaa gacttataat tgtatataaa gctatagtat aactagagtt
    23761 attaagtata aagaatatct acttgattaa tgcaatatag ctggcaaata tacaattgat
    23821 aaggagtaat tttataatgg ataatttaat taggatactg atattagctg ttattggtgg
    23881 atttataggt tatgttacga atgtagttgc tataagactg atatttagac caatagagcc
    23941 tattaaaata cctattttaa atatagaaat agtaggtctt ataccaaaga gaagagcaga
    24001 aatagcagca aatgtaggag aaataattca agaagaattt ctttctatgg acgaaatact
    24061 tgcaaatatc attactgacg aagataagga agaagttgta agatacataa aagcaagggt
    24121 aaaaattata atacatgaga aagtttcatt tatcccgagt ggtataaaaa acatgataca
    24181 agattatcta ggagagataa ttgagtctga agttaagcaa agtatagatg aattgagtaa
    24241 aaatataata aataaagcaa atgaacgtat agatatacaa aaaatggtcg aagataagat
    24301 taatgaacta gatttatatg aattggaaga gataataata agaatagcta agaaagaatt
    24361 aaaacatata gagtttttgg ggcttgtctt agggttttta ataggtatag ctcaagggtt
    24421 aattactatg tttatttaaa tatatgagaa gtagcattta tttaagtact aattatattt
    24481 acctatttga gtgaattact tattaataac atttagatat taattgtatt tacctatttg
    24541 agtaaattac ttattaatag catttagata ttaattgtat ttacctattt gagtgaatta
    24601 cttattaata gtatttagat attaattgta tttacctatt tgagtgaatt acttattaat
    24661 agcatttaga tattaattgt atttatttat ttgattgtgt tacttattaa tagtatttaa
    24721 gtatttactg tactacttat taaccatatt aagtattgat tgtatttaag tatacaaaac
    24781 ctaaaaagag tacaaaaatt agaagttagt tttaataaaa attagtttta gtatttatct
    24841 agtcatatct atcatgcatg tggatacgtt ttaaaaattt taaatttatg aaaatattat
    24901 aatataacta taaatcaaga gattaattta atataaaaat ataagaatat tggtttacag
    24961 ctgattgaga tgaaaaataa tgggtaatta atatattaca ttaatgttga ttgatgtata
    25021 actatctgtt ataatgaaaa tatattaaaa atcatagtgt tgaagaggaa gagtagagta
    25081 gaaaccattc tcagagaatt tgggtctatg gctgagagcc catgtaatag gtctatttga
    25141 aaactacctt ggagctgagc ttattagcag tatttgtatt tatactatac gtgaaagtgc
    25201 tcgggtgacg accttatagt catcaagagg gttgtatata cttgtatacg ccaacttggg
    25261 tggaaccgcg aattaatatc gtcccaaaca tatttggggc gattttttat tacaaaaaat
    25321 ctaaaaatta tttcaataag gaggaaaaaa caatgattaa agttgcttta aaagatggct
    25381 caataaaaga atttgaaaat gctatttctg ttatggatgt agctaaatcc attagtgaag
    25441 gattagctag aaatgtggta gcagcgtctg tgaacggaga ggttgtaggt ctggaccaca
    25501 ttatagatac tgattgtgac ttgaatttat ttaagtttga agataaagaa ggtaaggaag
    25561 tttttagaca tacatcagcg catatattag ctcaagctat aaaaagactt tacccagaag
    25621 caaagcttgc tataggacca agtatagaaa atggattcta ctatgatata gatttagacc
    25681 atagattagt tccagaggac ctagagaaaa tagaagctga aatgaaaaaa atagctaaag
    25741 aagatttaaa aatagaaaga tttgaacttc ctagaaatga agctttagag cttatgaaag
    25801 aacaaggaga agactataag gtagaactta tttctgattt acctgaaagt gaaataatat
    25861 ctttctacaa gcaaggtgat tttactgatt tatgtagagg accacattta ccatctacta
    25921 aaaaggtaaa agctgttaaa ttacaaagtg tagcaggtgc atattggcgt ggtgacgaaa
    25981 ataataagat gcttcaaaga atatatggaa cttcttttga gaaaaacaaa gacttagaag
    26041 agtacttaca tttactagaa gaagctaaaa agagagacca cagaaaatta ggtaaagaat
    26101 taggattatt tatgatacct gaagaaggtc caggattccc tatgttttta ccaaaaggta
    26161 tggaacttaa aaatgaatta ttaaaattct ggagagaaat acacagaaaa gctggatata
    26221 ttgagataga atctccaatc atattaaata gaaaattatg ggagacttca ggacactggt
    26281 atcattacaa agaaaatatg tatacagtta aaatagatga tgaggattat gctataaaac
    26341 ctatgaactg tcctggtgga ttaatttact ataactctca actacattca tatagagatt
    26401 tcccaatgag agttgcagag cttggtagag ttcatagaca tgagttatct ggtgcattac
    26461 aaggattaat gagagttaga gcatttactc aagacgattc acatatattc atgttaccag
    26521 agcaaattaa ggacgaaata aaaggagtag ctaacttaat agatggtata tacaaaactt
    26581 ttggatttga atataattta gaattatcta caagaccaga gaactctatg ggaagcgacg
    26641 aagagtggga agcagcagaa aatggattaa gagaagcttt agaagagtta ggtctaccat
    26701 atactattaa tgagggtgat ggagcattct atggtcctaa gatagacttc catctaaaag
    26761 attgtttagg aagaacttgg caatgtggta ctatccaatt agatatgcag cttccaagac
    26821 aatttgataa tacttatatt ggtcaagatg gtgaaaagca tagaccagtt atgattcata
    26881 gggttgcttt tggtagtata gaaagattta taggtatatt aatagagcat tatgctggta
    26941 aattcccagt ttggttatct ccaactcaag ttaagatact tcctatatct gataaattta
    27001 tggattatgc aaatgaagtt aagaaagagt tatttgataa gggtataaga gttgaattag
    27061 atgatagagc tgaaaaaatt ggatttaaga taagagaagc tcagcttgaa aaagtgccat
    27121 acatgttaat agtaggtgaa aaagaagtag ctgataataa tgtttctgtt agatcaagag
    27181 ataaaggtga aataggtagt attaaattag atgaatttat agctagtatt tcaaaagaaa
    27241 ttgaatcaag agaaagtatt attcaagact aatatatatt atattagtag ctaagatatt
    27301 gttatataag ataagtttaa tcaatatgtt gatatgttga tgcatagtga ttaaatttat
    27361 gaaaagatga gtataattag aggtacagtt aatcatattt ttatgattaa tgtacctcta
    27421 gttttttgta tggtagattt attaataaat atatcataga agtgttttga acatggaaat
    27481 atttatgtgc attctttaaa taaaagttta ttttttgagt gataattaag tgcttgttgg
    27541 taaggttatg gctgtaaaaa tcataacttt attttcatat ttgttccatt ttcataaaaa
    27601 ttattatccc tttaagaaaa tttcttaatc ctcttgaaat atttcctttt tagtctaaag
    27661 ttaaatttct ttcttcaaaa tgaatattta aatgaactaa aatttaagta cattttctaa
    27721 tcttatgagt ctatttttca tagataattg ccctataata gaagaatcta tgttggtgct
    27781 atgtggtgac aataacagca ttgtcagtga ttaattaatg ctttataaat gattaattag
    27841 tgttttatgt tatttttgga cttatcctct atacatagat taatttattt aactttagat
    27901 agagagagaa tgtcaagtac atacacagac attagagttt aagaaagtgt ttatggagca
    27961 agaactttta aaagaggtag tgacttagtt taatttcaag aaatgataaa tacaaaagag
    28021 gaaaaagaga ggtgttgaaa tgatagaaaa agtagaagaa aaatctaaga ttattaaaac
    28081 atcaaaagaa tataagcgaa ctataattga agaaaaagat aaagaatgga ccaaaaaatt
    28141 aaatgatata cttagtagaa gtagtttttc taatgatgaa ttagataaaa tcaaggcatt
    28201 aattccaaaa gagattttga ctaatgaaaa tgttggagaa gttgtgactg aagatggata
    28261 tgctaagcct gagtatgatg aggtatttaa atcattgttc actttaaata atgaatatga
    28321 tttacttgta aattttataa atgatattct aaaagatgct ccatatgcta acaggaatat
    28381 caaaccgttc actcaaataa agagaataat tagagttgaa actgatccaa caattaatta
    28441 cattggagag aaaagaccta aacttgatat tttggctgaa gatgaagaaa ataatcatat
    28501 aaatattgag atgcaaagag cttttgagaa cgattatttg gaaagagctg aatattatct
    28561 tagtcgtgta catggtagaa agttagaaga aggtaaagag tataaagaaa ttggaaaaac
    28621 tattggaata catatactaa atcatgtaaa atataatcac atagaagatt atgtaaattg
    28681 cctgagatta acaatggacg ggcatccaga tatttattct agtaaaactg cattatattt
    28741 tatagagctt ccaaaaattc ataaatctga ctatatagta aatagaatta tgctatgggg
    28801 aaaacttatt agtaatcctt ctaacttaga tgtaagagtt atagctaaga atgattctat
    28861 tattaaaaaa gcacttgata gattaaaaga attaggtagc aatgaagaat atttattaaa
    28921 tctgaaaaga ggtgcttata tcatgaataa aagtagaaat tttaaagaag agattgaaag
    28981 agagacagca attagagttg caaaagaaaa agattataaa ataattataa tgttgaaaaa
    29041 aaatggtttt aaattagatg acatattaaa taaatttaat gattttgact taagtgaaga
    29101 tgagataaaa gaagtatatg aaaataattg atgataaata aaaagtgaac ttatataagt
    29161 ttgctgaatc ggagtctgga aatatttata tgtatttttt aatgaatgaa aatttatttt
    29221 aaaaacaata atttagaagt acagttaatt atatttttat gatactgtac ttctagtttt
    29281 ttctattgta gatttatta
//
LOCUS       DAYASV010000036        28869 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYASV010000036 DAYASV010000000
VERSION     DAYASV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.036"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            1..889
                     /locus_tag="ACGI68_001599"
                     /pseudo
     CDS             1..889
                     /locus_tag="ACGI68_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            897..2897
                     /locus_tag="ACGI68_001600"
     CDS             897..2897
                     /locus_tag="ACGI68_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138042.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            3159..4418
                     /locus_tag="ACGI68_001601"
     CDS             3159..4418
                     /locus_tag="ACGI68_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0138043.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            4479..6266
                     /gene="iorA"
                     /locus_tag="ACGI68_001602"
     CDS             4479..6266
                     /gene="iorA"
                     /locus_tag="ACGI68_001602"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HFH0138044.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            6268..6843
                     /locus_tag="ACGI68_001603"
     CDS             6268..6843
                     /locus_tag="ACGI68_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HFH0138045.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            6896..7966
                     /gene="buk"
                     /locus_tag="ACGI68_001604"
     CDS             6896..7966
                     /gene="buk"
                     /locus_tag="ACGI68_001604"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HFH0138046.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            8184..8885
                     /locus_tag="ACGI68_001605"
     CDS             8184..8885
                     /locus_tag="ACGI68_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0138047.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            8945..9451
                     /locus_tag="ACGI68_001606"
     CDS             8945..9451
                     /locus_tag="ACGI68_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFH0138048.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            9626..10648
                     /locus_tag="ACGI68_001607"
     CDS             9626..10648
                     /locus_tag="ACGI68_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0138049.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(10795..11103)
                     /locus_tag="ACGI68_001608"
     CDS             complement(10795..11103)
                     /locus_tag="ACGI68_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFH0138050.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(11163..11591)
                     /locus_tag="ACGI68_001609"
     CDS             complement(11163..11591)
                     /locus_tag="ACGI68_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138051.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(11814..13271)
                     /locus_tag="ACGI68_001610"
     CDS             complement(11814..13271)
                     /locus_tag="ACGI68_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFH0138052.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13689..14603
                     /gene="nadA"
                     /locus_tag="ACGI68_001611"
     CDS             13689..14603
                     /gene="nadA"
                     /locus_tag="ACGI68_001611"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFH0138053.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            14616..15917
                     /locus_tag="ACGI68_001612"
     CDS             14616..15917
                     /locus_tag="ACGI68_001612"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFH0138054.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            15898..16734
                     /gene="nadC"
                     /locus_tag="ACGI68_001613"
     CDS             15898..16734
                     /gene="nadC"
                     /locus_tag="ACGI68_001613"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFH0138055.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            16990..17322
                     /gene="saoD"
                     /locus_tag="ACGI68_001614"
     CDS             16990..17322
                     /gene="saoD"
                     /locus_tag="ACGI68_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE-related protein SaoD"
                     /protein_id="HFH0138056.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            17412..17669
                     /gene="saoT"
                     /locus_tag="ACGI68_001615"
     CDS             17412..17669
                     /gene="saoT"
                     /locus_tag="ACGI68_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_240067706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:17445..17447,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-like (seleno)protein SaoT"
                     /protein_id="HFH0138057.1"
                     /translation="MSKILVEFINTUPSCDEHGVFIKKMSEKYADKLEIKLYQAGKDF
                     SYIKKYGIITKGTLIINQKKKYDRLNKDTIERAIVEAINNN"
     gene            17696..18781
                     /gene="saoE"
                     /locus_tag="ACGI68_001616"
     CDS             17696..18781
                     /gene="saoE"
                     /locus_tag="ACGI68_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter SaoE"
                     /protein_id="HFH0138058.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMGNMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            18774..19232
                     /gene="saoC"
                     /locus_tag="ACGI68_001617"
     CDS             18774..19232
                     /gene="saoC"
                     /locus_tag="ACGI68_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Cys-COOH (seleno)protein SaoC"
                     /protein_id="HFH0138059.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            19267..20358
                     /gene="saoX"
                     /locus_tag="ACGI68_001618"
     CDS             19267..20358
                     /gene="saoX"
                     /locus_tag="ACGI68_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding subunit SaoX"
                     /protein_id="HFH0138060.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            20372..21160
                     /gene="saoB"
                     /locus_tag="ACGI68_001619"
     CDS             20372..21160
                     /gene="saoB"
                     /locus_tag="ACGI68_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:20585..20587,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     (seleno)protein SaoB"
                     /protein_id="HFH0138061.1"
                     /translation="MKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTSGFV
                     INYMVNRDYFENIKVSDIMDIFTINDCUASTMQWALSSEEIDIAIICKDAAKQYVNVN
                     SGFEIVGTVVQNSDIFLLSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAY
                     GLESNLVDGVVIDCIKSIGLEGKRKSTTKLGDYDTYVLVVNKEFKKSKAYSDFITLYN
                     KSVDALKNKATFKKVLKEYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            21184..21927
                     /gene="saoP"
                     /locus_tag="ACGI68_001620"
     CDS             21184..21927
                     /gene="saoP"
                     /locus_tag="ACGI68_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_239591060.1"
                     /note="Most members of this family are selenoproteins with
                     the selenocysteine residue at the channel-gating position;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:21748..21750,aa:Sec)
                     /transl_table=11
                     /product="ABC transporter permease subunit SaoP"
                     /protein_id="HFH0138062.1"
                     /translation="MDILYKIILLVIIFLVWHFAAKDIGSSLLLPMPVDVIKGFFFCV
                     TDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFSKTFERVLSPVVDSVRQVPIMA
                     WVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVRAISKDYYNAARSMGASPIVIF
                     TNVIVPASLPDILTGSRIAISTGWMSVIUAEFIATSAGLGYSMVQAQTLMHTDVLIGL
                     MIFAALIGFIIDRVLKFINKILCRWRFAD"
     gene            21927..22679
                     /gene="saoA"
                     /locus_tag="ACGI68_001621"
     CDS             21927..22679
                     /gene="saoA"
                     /locus_tag="ACGI68_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein SaoA"
                     /protein_id="HFH0138063.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            22692..23333
                     /gene="saoL"
                     /locus_tag="ACGI68_001622"
     CDS             22692..23333
                     /gene="saoL"
                     /locus_tag="ACGI68_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_238476746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018942 - organometal metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:23175..23177,aa:Sec)
                     /transl_table=11
                     /product="MerB-like organometallic lyase SaoL"
                     /protein_id="HFH0138064.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYVSKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     CUNIMNFFWTKSDFDAWTNEAGLSDDEDIYCLDINEAIVESYKIFKLKQKVLS"
     gene            complement(23469..24290)
                     /gene="yidA"
                     /locus_tag="ACGI68_001623"
     CDS             complement(23469..24290)
                     /gene="yidA"
                     /locus_tag="ACGI68_001623"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFH0138065.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            24783..26477
                     /locus_tag="ACGI68_001624"
     CDS             24783..26477
                     /locus_tag="ACGI68_001624"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFH0138066.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYIRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      26809..26919
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            27023..27394
                     /locus_tag="ACGI68_001625"
     CDS             27023..27394
                     /locus_tag="ACGI68_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HFH0138067.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            27489..28436
                     /gene="trxB"
                     /locus_tag="ACGI68_001626"
     CDS             27489..28436
                     /gene="trxB"
                     /locus_tag="ACGI68_001626"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFH0138068.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            28540..28857
                     /locus_tag="ACGI68_001627"
     CDS             28540..28857
                     /locus_tag="ACGI68_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HFH0138069.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT        11318 a         3045 c         5226 g         9280 t
ORIGIN      
        1 aaatgttttg gagatgtttt tatatataat tctaaactag attacatata taagttgtat
       61 ggagatttat atataaaatt tggtaatata gatgaaggta taagttgtta tataaaaggt
      121 ttaaatttag ttaaaaagag ttcattgaat cttagaaaaa tatctatatt tatatgggct
      181 aatagcaaat ggatatgagc taaaaaaaga ttttaaactg gcaatagaat attataaaag
      241 tatggattgg tattctgata ggtgggaaaa aaataattcc tataaaacat ctcaagcaat
      301 tcacaaacaa tatgaactca gacaaaaaca agagagttta agattactag tggatgataa
      361 taagaaatta gaaaatgata ttttaaaaga tggacttact aaactatata atagaagata
      421 tttggaacag cagttggatt tatttaatgg aaccagagac aaaaatatgg tcgcaatact
      481 ggtcgatata gattgtttta aaagttataa tgataattat ggtcatctag caggggataa
      541 agttatagtt aaggtaactg atattataag aagtgtattt atagatatta ccgaacacat
      601 aatcagatat gggggagaag aaattttaat attagtagat tctatagaat caatcggtgt
      661 tgaagagatt gataatatag aattatatat taaaagagtt ttcaaacttc ttgaatttga
      721 gggtattgaa catatatatt ctaaaataaa aaactatata actataagtg ctggagtaag
      781 tataaaaagg tgtcgtagta gaaaagatgt tgaaatatta attgaagatg cagataagaa
      841 cctatataaa tcaaaaaaag caggtagaaa tcaatacatt ataatttaaa attgaggtga
      901 ttttgttgcg aaaaaaaata ggaattatag cctctgatat tgaattaaaa gaaagaatag
      961 aagaattata tagagaagat gtagaaaatg gaactataat aatcgacata cttaacctag
     1021 acttaatgga aaatcaaggt cgtatacttg ttgaaaaagg tgcccaagct ataattggta
     1081 gaggtggagg ttatagctta gtaatagata cagtaaatgt acctgttata ccaatgaata
     1141 tgaagtcaac tgacctttta agagcaatag aaatagcaaa gaaatattct aaaaaagtag
     1201 tattgattct tggagacaat gaagtttctt ttgactatgt tggctggaga aatgtcataa
     1261 gtactgaaat aactgaagaa tggtttgaat ctaagtatga gataagaagt aaagttgtaa
     1321 aatatataga ccaaaaagat gaagttgtaa tcgtaggagg aggtttggca tgttcttttg
     1381 caaggcaata tggaattgat agtgtatttg caactgctag tgatgaatct ataagagaag
     1441 ctgtagaata ttgtaaaaaa ttattagata cattaggtga agaaaagttt aataatgaag
     1501 tgttacgaaa tatacttgat ggtataaaag atggagtaat tgctatagat agtaatggca
     1561 gtataatttt gtacaatgaa agtgcaaaaa acatgcttaa agtagagaga aaatgtgcac
     1621 ttaataaata catcttagat gtatttccta aaatggagtg gatgttagat tgtttacatg
     1681 aaaaagagga cgtagaagat agaaagataa gaaatataaa taatctaatt gttaatacta
     1741 ggacaacatt gataaaagtt gataatagta cttatggagt tttaggaata atacaagata
     1801 taaccaagct acaaaactta gaaagaaaaa taagatttga cttgaatcaa aaaggtctct
     1861 atgctagata tacttttgat gattttttat ttaaagataa attaactaaa gaatttattg
     1921 aagaagctaa aaaaattgga aaaagtgatt atacaacttt actttatgga gaaagtgggt
     1981 ctggtaaaga aattatagct catagtatac acaacataag taaaagaaaa gataggcctt
     2041 ttgttgctat aaattgtgct actatagcag aaaatttact tgaaagtgaa ctttttggct
     2101 atgaagaagg tgcatttact ggtgctagaa agggtggaaa aagaggacta tttgaacttg
     2161 ctcatggagg gactcttttt ttagatgaaa taaatagtct atcctttaat attcaaacaa
     2221 aactattaag agtaatagaa gaacgtcaaa taatgagaat tggttctgat tatataatac
     2281 ctctggatat aaggattata gcagcaacga atgaatcttt aaccgaaaaa atagttatgg
     2341 gtacatttag agcagactta ttttatagat tgagtagttt agagataaat atacctcctt
     2401 taagagacag aagagaagat ataatacctt tgtttaacaa ttttgtaaat gaagtattaa
     2461 aggatgatgg tctcaatgga ataaacagta tagatgaaaa ttttgttttg actaaagatg
     2521 aaatggataa gctatataat tatagctggc ctggaaatgt aagagagtta aagacaatag
     2581 ctcaaaaata tgttgtgaca ggtaaaatta aattgagaca agatagagat tttaaaacta
     2641 aaaaattact tcctaactct gaagtagaca aatttaattc tgaaacgaca gcaagtgtag
     2701 aagtccaaga tgaatctata aatataagta agataaatga tggtaagata agtatagata
     2761 taaaagaagt aaataagtat gttgaagaaa aaataataag tatgcttttt gctcagggac
     2821 tttctaaaaa tgaagttgca caagttcttg gaataagcag gacatcttta tggaagaaat
     2881 acaataaaaa tatttaaata taattacata taaattattt tgaagtaatg ttaatttttt
     2941 aaaacaagtg ttaagaaaaa ttaacattac ttttttgcaa atttttattt ttttagacag
     3001 gcatatatat ttataagaaa aatagttaaa acacttaatt taataatgaa aaaaataata
     3061 attctaaaca aaatcaattt tacataaagt gggatttatt ggcatagtaa ttgcttaata
     3121 tataatgtaa gagtaaatac aattttaggg gtgatggtat gagtcaaagt atggttggta
     3181 agcatgcaat gtggccaaaa gaaaatgatg ttatatttag catatctggc agagctcaag
     3241 cagcagagaa ggcttttggt atggataatg taataaatgc aacaataggc gctctaatgg
     3301 atgattcagg aaaattgata acaatgaaaa ctgtatatga agaatataaa gcattagata
     3361 actgtgagat aggtgcatat gcagctcttg aaggtcaacc agattactta gaagcagtta
     3421 aaaaagtatt tttcagagat tacttaccag aaggacatat aagagtatta gcttcaccag
     3481 gaggaagtgg tgcaataaaa ttagctgtat ggaactatac taatgaagga gatgaggtat
     3541 taacatctga ttggttctgg agtccatatg taagtatagc tgaagaggct aatagaaaag
     3601 tagttaatta ccaacttttt gatgaaaata gaaggtttaa ttttgagtcg tttaaagaaa
     3661 aatttgtaaa tatagcagaa aaacaaggta gagtctttac aattataaac acaccagcgc
     3721 ataatccaac tggatatagt gttgcagatg atgagtggga taaaatatta gacttatcaa
     3781 aagaagtcgc aaaagataaa gacaaaaaga taatattttt cgtagattca gcatatattg
     3841 actttgcagg tgatgatgat gtatgtagaa agttttttaa gaaattttct aatttgcctg
     3901 aaaacgttct cgtattggtt ggattcagta tgtcaaaagg atttacagct tatggtatga
     3961 gaatgggagc tataatttgt atatcttcaa gtgaagatgt agctgaagag ttccattact
     4021 catgtgttca ttcatgtaga gcaaattggt caaattgtaa tagaagtgct atggcagtat
     4081 tatctaacat tgtaaatgac cctaaaaaat tcaaagaata tgaggatgaa aaagaaatat
     4141 acaaaaacat gcttactaga agagcagatg tatttgtaaa agaagctgaa agagtaggat
     4201 tagaaattct tccatatata gcaggtttct ttgtaagtat accatgtgat aatccaaaag
     4261 aagtatgtga agaattgact aaacataatt tatttgcagt tccattaaaa atgggcttaa
     4321 gatttgcagt ttgtgcagtt tctgaagata aatgtaagaa agcaccaagt attataaaag
     4381 aagcacttga aagtttagaa gtaaaaataa ataattaatt tcattgaaca aattgggtgg
     4441 actatgaatt taataattgt agatggaggg aattttatat gaaacaattg atgacaggta
     4501 atgaagcaat tgcacgtgga gcctatgagg ctggtgttaa atatgcatct gcatatccag
     4561 ggactccaag tacagaaata ttagaaaata tagcaacata taaagatgca atagttgcag
     4621 agtgggctcc aaacgaaaag gtagcacttg aggctgctat agggggttca attgcaggag
     4681 caagaacaat ggcttctatg aaacatgttg gtttgaatgt tgcagctgat ccaatattta
     4741 cttatgcata tacaggggta aatggtggta tggtacttat tacagcagat gagccgggaa
     4801 tgcattcatc tcaaaatgag caagacaata ggatgtatgc aaaatttgca aagatacctt
     4861 tatttgaacc atcaactagt caagaagcta aagatatgat aaaagaagct tttgaagtta
     4921 gtgaaaaata cgatacacca gttttatata gagttacaac aagactttgt cattcaaaag
     4981 gattagtgga gtgctatgat agagaagaag ttgaaattaa agagtatgtt aaaaatgcta
     5041 agaaaatggt aactgttcca gcaaatgctc aaataagaag gggcgttgtt gaagaaagaa
     5101 tggaaattct taaaaaattc tcaaatgaaa ctgatttaaa ctactatgaa ataaatgata
     5161 caaaaatagg tgttatagca tctggtatgt gttgtaattt tgccaaagaa gtttttggaa
     5221 aaaatgcatc atatatgaaa ctaggattta caaatccatt accttatgaa aaaataaaag
     5281 aatttgcaga aaaagttgat aaaatatatg ttatagaaga aaatgaccca tttatagaag
     5341 aacaaataaa agcatatgga atagattgta ttggaaaaga tgtaatacca ccatatggag
     5401 aaatgacacc agatgtttta agaaaagcta tatttggaaa aacaaatgat actatagaat
     5461 acaaatctga attagtaaca ccaagaccac caagtttctg tgcaggatgt ccacatagag
     5521 gattcttcta tgaacttgga aagagaaaaa atttaatagt tggtggagat ataggttgtt
     5581 atactcttgg atttgctcct ccatataatg gaatagatta tgttgtttgt atgggttctg
     5641 cttttggaac tgcacatgga gctcaaaaag ttcttaatat gaaggatgat aatgaaaaaa
     5701 gattggtagg tgtacttgga gactcaacat tcttccatac aggaataaat ggacttttag
     5761 atgttgttta caatagaggt aattctattt cggttatatt agataataga ataactggaa
     5821 tgacaggtca ccaagaaaac ccaggttcag gatatacttt acaaggtgct aaaactaaag
     5881 aagttgatat agaaggatta gttaaagctt gtggaataga acatgtaaga gttataaacc
     5941 ctaataattt aaaagaagta aatgaagcac tagattgggc tttagctata gaagatgaac
     6001 catcagtaat aataacaaga tggccatgtg tacttaaaaa attctctaaa gaagatatag
     6061 aagaatttaa taatccattt aagactaagt gtaaagttga ccatgataaa tgtataggat
     6121 gtaaattatg tttgaaaact ggatgtccag ctctatcatt tgataaagag aataagttat
     6181 caaatataga tagaaatcag tgtgtaggat gtggagtatg tgcacaagtt tgtccaaaac
     6241 aagcaatagt taaggaggaa aaataatatg actaagagta tacttttggt aggtgtagga
     6301 ggtcaaggaa ctatacttgc tagtaaatta ttaactatgg gattgatgga agctggatat
     6361 gatgtaaaga tgagtgaaat acatggaatg agtcaaagag gaggttcagt ttcctcacaa
     6421 gttagatatg gagattgtgt tcattctcca gttatagaaa ttggtggagc agatatatta
     6481 gtatcttttg aaaaaatgga agctttaaga tggttcaatt atttaaaacc agaaggaaaa
     6541 gctttagtga ataatcatag gatagattct atgactgttt taatcggagg agctgaatat
     6601 caagaaagtg atatagatgc agagttaagt agattaaatg caaaagttat aaatgcagct
     6661 gataaagctg aagaactagg aaattcaaaa ataatgaatg ttattttatt aggatgtcta
     6721 gttaaatcta tgcaattaga gtctattgat tgggaaaaaa ttataagtga aaatgttaaa
     6781 ccaaaatttg ttgaattaaa cataaaagct ttccatgaag gtatggaaat ggttagcaaa
     6841 tagtttataa tagatataaa tagactatag aaattaaggt gaaaggaaga aaattatgac
     6901 ttacagaata ttagccataa atccaggttc tacttctaca aaaatagcag tatatgatgg
     6961 agaagaacaa attcttgtga agacgataga ccatccagct gaagagattg caaaatataa
     7021 tactatacaa gaccagtttg aaatgcgtaa ggaagcagtt ttgaatattc ttaaagaaaa
     7081 tagtatagac ttaaaatctc ttagtgcaat agtaggaaga ggtggagttt taccaccagt
     7141 aaaatcagga gcatatttag taaatgaaga aatgattgat gtactaagac atagaccagt
     7201 acttgaacac gcttccaatt taggtgctgt tgtggcacat gcaatatcag aacctcttgg
     7261 aatcaactca tatatttatg attctgttgc agtagatgag cttatagatg tagcgagaat
     7321 atctggactt tgtggaatgg atagatcaag tgcagggcat gcattaaata ctagagcaat
     7381 ggctttaaaa tatgctaagg ataaaggaaa agattataag agcttaaact taatagtagc
     7441 tcacattggt ggaggagtaa gtatttatct tcatgaaaaa ggaagaatgg ttgatatgct
     7501 atctgatgat gaaggaccat tttctccaga aaggtcagga agagtacctg ctacaaaatt
     7561 agtggctgcc tgttattcag gtcaatattc agaaagagaa atgactaaaa agataagagg
     7621 taaaggtggt atagtttcat acctaaatac tgtagatgct agagaagttg aaaaaatgat
     7681 agcagaagga aatgaagaag caaaaattat ttatgaagca atggcttatc agttagcaaa
     7741 aggtattgga gagttagcaa ctgtagtaga tggaaaggta gatgctataa ttataacagg
     7801 tggaattgca tattctgaaa tgtttacttc aatggttaaa aagaaagttg agtttatagc
     7861 accagtagaa attatggcag gagaaaatga gttggaatca cttgcttttg gaactttaag
     7921 agtactaaat ggagaagaag aagctagaat ttatagtgaa aattaataag aaaaactaaa
     7981 aatgtttata tactaaaaaa gtggtatatc cgttttttat acacgaaagc ttagagtata
     8041 ggtgaaaatg tataaaattt ggattgccac tttttataat taaaatttta tcttaaccta
     8101 ataaataatt ctgtaaaatt tcgtatttat atatatattt tattggtatt tattgttatt
     8161 attattatga ggagtgattt ttgatgggaa aaataagttt aaaaagtatg ataagattat
     8221 tttcaggttt ttttatattt gcaatttctt ctgtgcttat gattaatgca catgtaggtc
     8281 ttatgccttg ggatgtactt caccaaggtt tatctataaa gctaggcata actataggtc
     8341 aggcttctat aatggtagga gttgtaattg ttatattgga tgcagttttt ggtgaaaata
     8401 taggatgggg tactttactc aacatgacat ttataggaat ttttattgat ttagtaatat
     8461 tttctggagt gatacctcat gcaagcaaca cttatatagg tgtgtttatg gttgttatag
     8521 gaattatttt ggcagctata gcaagttttc tatacttagg tgtttgttta ggaagtggac
     8581 cgagagatgg gcttatgatt gctcttcaga aaaaaactaa taaatctgta cgtcttgtta
     8641 gaacaattct tgaaatacta gcattagttg ttgggtggtt attaggaggt tctgtaggta
     8701 ttggtacgct tgtaagtgct ttaggtttag gatatgtatt acaaatagtg tttagaatat
     8761 ttaaatttga cacaaaattg ttgaaacata gatttattat agatgatata agagaatgga
     8821 aggaaaagaa aagcaatgaa cataaatgta aatcaagtat tgtaataaaa aatgagcaga
     8881 attaattatg ctaaaatata tttgtagttt atctaattta tagtaaagtc aaggagattt
     8941 tgaaatggaa ttatgggatt tatataatgc tgatggaatt aaaacaggaa atgttataaa
     9001 aagaggtaat tctattgaag aaggttatta ccatttggca gttgaagtgt ggattttaaa
     9061 tagtaactct caaattttaa ttcagaaaag gtctaaaagt aaaaagacat tgccaaatat
     9121 gtggggaatg acaactggat gtattgtttc tggggaagac agtttagaag gagcaattcg
     9181 cgaagcaaag gaagaaatag ggatagatat aactaaagat gagatgaaag tttttagaag
     9241 tatgattcat gaagatactc tttgggatgt atacttagtt aaaaaagaat atgatatatc
     9301 caaagctatt ttacaggagg aagaagtaag tgatataaag tgggtaagca cagatgaaat
     9361 aagacaatta ctaaaagagg gtttgttttt tgaatatcca gagatatacg aattgctata
     9421 tgagatagat aataataaat taaatatatg atgtagagca gatttcagta taagtaagat
     9481 tatttaaaat gtatcttctc taaattttaa tattgtttta tgaaaattaa taagttattt
     9541 ttagtatttt tctacttgtt ttttgagatt aaaataaaaa gggcagtcat tataaagtca
     9601 taaaaatttg gggaggattt aaactatgga agataacaaa ggattggcac gtgacagttc
     9661 aaaatataaa attgtttcta tatctgtcgt agtaattatt ctaattacat tttggtatat
     9721 gttaaatatg gtgcttttaa catttattat gacttttatt ttctataact tgcttgtagc
     9781 cacaagaaaa cgaattaaga agttttcttc tttaaatatt ccagattcat taataattat
     9841 agtgttatat gcactatttg ctatattgtt agtattgatt agctatgcag ttgtaccaat
     9901 aattatagtt caacttacag agttgagtag ggtttttagt gattttgatg taaatcaatt
     9961 tgctcaatct ttaggaccaa aattgtatcc tattgtctca aagctggatt ttaataaata
    10021 tatttcacaa gcagggctac ttatagcatc aacagcaaca aaagttggaa gttttggagt
    10081 aaatatatta ttggcatttt tactgagttt actcctttta cttgaaaaaa atgaaattaa
    10141 aaattttgga gataaacttt ctgatagtaa gatttctttt atttataatt cgcttgtatt
    10201 ttttggaaag agctttgtta aaaattttgg tgaagtaatg aaagtacaag ttatgattgc
    10261 ctttataaat tctgttgttt ctatgatttt cttaggtttc atgggattcc ctcaaatatg
    10321 ggctttagga tttatgattt ttgtgttggg tcttattcca gtagcaggag ttatagtgtc
    10381 tttgattcca ttaactgtaa tagcatttaa tacaggagga ataacaaagg tttttggagt
    10441 tttacttatg atttgtatag tgcatgcagt agaaacatac atattaaacc ctaagttgat
    10501 gtcgaataga actaaacttc cagtttgttt tgtttttatt attttacttg taggagaaca
    10561 ttatttaggt gtatggggat tactaattgg agttccaata tttatgttcc ttatggatat
    10621 tctgggtgtt aaatttactt ctaggtaata tattaaaata ataatttatc tttatatata
    10681 aattataaat tgttttaaaa aagaacctat aaatagacat cattaataaa gacatctatt
    10741 tgataggttc aattttgctt tgtataaata ctataaaact aaataaaaag aaatttattt
    10801 catttcaaaa gtttcacaac ttgcatttct ttcatttata tgaacttttt cagctttgca
    10861 aagttcattt ttattgtatt tacattttac agcttcacaa tgaatgttat ctgtatcaac
    10921 ttggttagtt tcattagaac gactagttat accagatgta tatttgtctt caaaagaagc
    10981 acaataagta ttttctgtag tagttgcttg cattccatct acttttatac taccagcata
    11041 acaatatcca ctagtattat aagcacaatt agtagcagca caatttaaat tattattttt
    11101 cataaataac ctcctaagtt ttatttaaac ttattatgtg aagttatcta aattttatac
    11161 acttatttac aattacattt atcacattta acaactacat ctttggaagt ttcccttcct
    11221 aaatttttat taacatgata cacatcaaca ttttcagttg atccacatat agaacattta
    11281 aaattgtttt ctagtagggt tctttcttta aataacttcc aatacataga attgatgtaa
    11341 tcattataat ttaccttgag gcatgtctca agaaagttta tgtatagatt gtctaaatct
    11401 cctgtattta attctgtata ttctggttca aaatattcac aacatcctct aaaatcgggc
    11461 tttgtatctg aaataacaca tctaatatca tatctatagt ctaggctagg ccccttaaat
    11521 gaaagatgtg tgcaaaaaga acaagatgtt tctggtagat ttttttcaag ttcatgtaat
    11581 ttattattca taagtattcc tccaaaaaat ttattttaag tatataaacg aatagattat
    11641 tttaaaactt ttatttattt atattagaga tttaggataa atattccaat aaggagtaaa
    11701 tacttaagta tttattactg tattaaataa aaaagaggca aatctaactt gagattgcct
    11761 acttttttat cattttttaa taaaatttat tctaagtatt attttattta gatttagtca
    11821 gccaacttag tatctgcttt gctttttaac tttctaccag agcgtacagt taaaatagca
    11881 tataatacag taaatatggc agctaaaaca tatgatatat ttatgtttaa gttaaatcca
    11941 atttgagcat taaatatata actgaatata acaaaggaat aaaacatacc aggaattaag
    12001 cttataatat aattcttttc ttttattata aggtaaactg taatcattgc aaatgcaaat
    12061 atggcaatag tttgattact ccaagagaag tatctccaca atatattaaa tccagaagca
    12121 ctaagctttg caaatataag gatggctatg acaggaataa atatacagat tgacaatata
    12181 acacgatgtt ttttttcctt ttggtcataa tgaaagtaat ctgcaagcat taatcttaaa
    12241 gatcttaaag ctgtatctcc agaagttatt ggtaaaacaa ttacacctat tatagctatg
    12301 ataccaccaa tagaaccaag taaatctctt gcaaccaaac ctattacatc aggagaacct
    12361 actaattcct taggtattcc tttattgtaa atacccatag cagctgcagc ccaaatcatt
    12421 gcaattaaac cttctaaaat catcatatta taaaaagtag tttttccttc tttttcattt
    12481 gaaactgatc ttgctataag agtagcttga gttgagtgga atccagaaac aataccacaa
    12541 gcaactgtaa caaagaatgt aggaataaga ggaataccat caggatgaat acctttccaa
    12601 tttgcaagac ttaaattagc taaatcataa ccttgtgtaa agattccaat tccaacacca
    12661 gcagcagata aaagtagaat agctccaaat attggatata cctttcctat gattttatca
    12721 attggaaata gtgttgcaca caagtaatat agaaagataa gtccataaac tatccagaca
    12781 actggattat ttatagtcga tttcatattt aaaatttgag taacaactaa atccccaggt
    12841 gtataaataa aaactgcacc aactaaaagc attaaaagac aaaggaatat attatagact
    12901 tgaaatactt tattacctaa aaatctactg ataagagaag gcatttgtgc tcctttttct
    12961 cttatactaa tcattccact catatagtca tgcaaagctc ctccaaatac acagccaata
    13021 ggtattagta tgaatgctat tggaccaaac aatatacctt ggataggtcc tagaattggt
    13081 cctgtaccag ctatattaag caactcaatt agactatttt tccatttttt cattgggaca
    13141 tagtctactc catcactttg agcaagtgca ggtgtttttc tgtcatctgg gccaaatact
    13201 ttttcacaat aagcaccgta taaagcacct cctattagta atatagccaa gccgattaag
    13261 aatgttgtca taatatcacc ccatttataa aattttttgg tctttgtata catattatct
    13321 aaatgtgtag atttatattg aatttaattc tggacaacct acaaattcta taaagtatta
    13381 caactaatat aatttcagaa ataaatacat atacataacc atgtacttag tatacaggta
    13441 tttggtaata ttttcaatgt tatagcaata aagaatatag acaaaattct gataatattt
    13501 tacaaaaaaa ttaaaaaata taaaaattag tttaaataga tggagaaaaa aggaaaatca
    13561 attgagaata taaatgtcta tatcttaaca atattaagag gtttaaaatt cttgaagaaa
    13621 gaatagtcaa gtgatataat gagtttacag gtgtatatac actagtacat atagaaagcg
    13681 gtgcaattat ggataaagat ttaacatatc aaatcaaaga gctaaagaaa gaaaaaaatg
    13741 caataatact tgcccatttt tatcaaccac cagaaataca agaattagca gatgcagttg
    13801 gtgactcata ttatttaagt gagattgcta gagattgtaa agaagaagtg gttgtatttt
    13861 gtggagtaag gtttatggga gaaagtgcta agatattatc acctgaaaaa actgtattga
    13921 tgccagtaag taacgctggg tgtgctatgg cagatatggt agatgaagaa ggcgtgataa
    13981 aattaaaaca acagtatcca aatgctttgg ttgtatgtta tataaactct acagctaaag
    14041 ttaaggcaca ttgtgatgtt tctgttacat cttctagtgc aattaaaata ctagaaaaca
    14101 tagataataa agaaattata ttccttccag ataaaaattt aggaggatat attgcagaac
    14161 aatttccaga taaaaacttt atattttggg acggatattg taaataccat aacaatataa
    14221 gagctgaaga aataattgag ttaaaagata agtataaaaa tgcagaagta ttagtacatc
    14281 cagaatgtaa aaaagaaata agagatttag gagattatgt aggtagtaca tcaggaataa
    14341 taaaatatgc tactaatagt aaaaacaaag actttataat agcaactgaa gaaggtatat
    14401 tacatgaatt aaagaaaaat aatcctaata aaaactttta tattcctggt ggaaaaatac
    14461 tatgtacaga tatgaaaaag acaacattag aaaatttata tagtacactt aaaaatatgg
    14521 aaaatgaagt tattgtagaa gatgaaatca tggaaaaggc tttaaattca ctgcttaaca
    14581 tgcacaaatt agcagaagga tagagggata gacttatgaa tttagaacaa gatgttttaa
    14641 tagttggaag tggagtttca ggattatact gtgcattaaa tttagataaa agtttaaatg
    14701 tgctagtagt atcgaaatca acaattgaaa acaacaatac atatcttgca caaggtggaa
    14761 tttcaactgc tagaaatatt gatgatatag aatcctttgt agaagataca atgaaggctg
    14821 ggcagtataa aaatagagtt gaagcagttc aagtacttgc agatgaatct atagaaaatg
    14881 tagggcagat agtagaatat ggtatgcctt tagacaaaga aaatggagag atagattaca
    14941 caagagaagg agcacatagt gtaaatcgaa tagttcatag taaggataat actggagaag
    15001 ttgtttttaa aactttatta aaagaagcta aaactagaga aaatataact cttatagaag
    15061 atgcctatct tttagatata ttaaaagatg gcaataaatg tataggagct agaattttta
    15121 aatctaaaaa agagattcat gttttttcaa aaatagtagt tttagctaca ggtggaattg
    15181 gtgggctttt taaaaattct actaatcaaa gacatcttac aggtgatgga attgcaatag
    15241 ctttaagaaa taatataaaa atagaaaatt tagaatatat tcaaatacat cctacagcct
    15301 tttatgaaga aaataatgaa ggtaggagaa tgcttatatc tgaatcatta agaggagagg
    15361 gagctaaact tttaaataaa aataaagaaa gatttgtgga tgaacttctt cctagagatg
    15421 tagtttcaaa agctattttt gagcaaatgg aaaaagataa attaccttat gtgtatttag
    15481 atgcaacaca tttagatagt gaatatttaa ttaatagatt ttcttttatt tataatgaat
    15541 gtttagctag aggaacagac ataacgaaag aatgtataaa ggtatctcct gcacaacatt
    15601 attttatggg cgggatacat gtagatttgg attcaaaaac atctatggaa aatttatatg
    15661 cagttggtga aattagttgt acaggtgttc atggagccaa tagattggca agtaattcat
    15721 tacttgaagg gcttgtattt tcaaaaagag cagctaaaaa tataaactca gttatagata
    15781 atgtaaaagt taagtttata gatgtaccag atatggatat agatatagaa caggtaaaaa
    15841 aagaaaataa aatattagtc ataaaagaaa ttgaaagaac aagtgaggat tttggcgatg
    15901 aattatttga ttattgatag aatgatacag gatgctttga tagaagatgt tccaagtgaa
    15961 gatataacaa ctaattctat tgtggatgaa aattctaaat ctactgtaga tttaatttgt
    16021 aaacaagatg gtataatagc tggcttaggt gtatttaaga gagtttttga gatacttggt
    16081 gatgtagatg taaaattata taaaaatgat ggtgatagag taaagaatcg tgaaaagata
    16141 gctgtattaa caggtagtac tagaaactta cttgtaggag agcgtgtagc attgaattat
    16201 ttacagagaa tgagtggtat agctactatt acaagtaggt atgttgaaaa gcttgaaggt
    16261 acaaatacaa aattattaga tagtagaaaa acgataccta atcttagaat attagataag
    16321 tattctgtta aagtaggtgg aggatgcaat catagattta atttatctga tggaatattg
    16381 cttaaagaca accatattgg ggctgctggt agtgttaaga aggcagttga attagctaga
    16441 aaaaatactt cttttgttag aaagatagaa gtggaagtgg aaactttaga catggtaaaa
    16501 gaagcaatag aagctaatgc agatataata atgttggata atatgagttt ggaaatggca
    16561 aaacaagcag tagatattat aaatggaaga gccattgtag aattttccgg aaatgtaaat
    16621 ttagatacta tagaagatat aggaaaaatt ggtgtggatg ttgtttctgt aggagcgctt
    16681 acacattctg ttaaagctct cgatataagt atgaagaatt tgagaaatat ataaatttta
    16741 tattgaaata aacttaatta aaatagactc cttaaatttg ttttataaat ttaaggagtc
    16801 tattttttat tcattaaaat taataaaaat agaaaaaaat caatcaataa tttaataaaa
    16861 atagataaat actatataca cttttaataa tatagttaat atctatttta cagataaata
    16921 agaataaact tataatataa ttgtatctgt aaaattttgt aaaatataaa aagtataggg
    16981 ggaataaaaa tgaaagtagc ttatattttt agtagtacta atacacataa aattctagac
    17041 aaaatgataa taccacaact tgaacaagga actcatggag tagacgttct agggatgttt
    17101 ttctttatgg ataatacctt atttttatct aaaggtaatc ctgtaggcga gagactaagt
    17161 aaattacatg aaaaaactga tatgataata atggcttgtg accaatgtgc tatagaaaga
    17221 ggtatagaga ataatttggt tgatggagct acaattggat gtttcccaaa cctatatgct
    17281 tgtttaggca gtgcaggtgt ggaccaagtt ataacgttat aaattagtat aggatacaat
    17341 ccatagaaaa atttattttc tgtggattat tttaagtaaa agataaattg tttaaagtag
    17401 gggattaaca aatgtctaaa attttagttg aatttataaa cacttgacca tcttgtgatg
    17461 aacatggagt gtttataaaa aaaatgtcag aaaaatatgc tgataaattg gaaataaaat
    17521 tatatcaagc agggaaagat ttttcgtata taaaaaaata tgggattata acaaaaggaa
    17581 ctttaataat aaatcagaaa aagaaatatg atagattaaa taaagataca attgaaaggg
    17641 caatagtaga agctataaac aataattaaa agtttaaaaa ttaggagtat caaaaatgaa
    17701 tatattagaa attgttttaa atggagttaa agatactata gtagcttcta tagttacact
    17761 gaatggagca tcaggatggc ttatgataag ttttatattg gcaggtgtac ttcacaatgt
    17821 tttaactcca agaaggtttc aaaaacaact tggaaataag aaattttctt ctttaattaa
    17881 aagtactatc tcaggaatgt ttttaccaat atgtagttgt ggagtaatac cacttggaat
    17941 aagtatgtat tattcaggag catatttagg accagtactt gcttttatga catcaactcc
    18001 aataataaat ccaatagctg tagttctttg tctaggtctt ttaggtccta aagttacaat
    18061 catttatcta atagctggtt ttttagtacc atttttagta ggatttttag gaaattgttt
    18121 tggaaaagaa gaattatcac ttcataatga ttcagaggaa gaagtaatag aattagaaga
    18181 agaaaaaaga agttttatgg gaaatatgat tgatggtctt aaatggtctt ttggtgaatt
    18241 agcccttact attagtaaat atgtagtaat gggtatggtt tttgcaggat ttataacaac
    18301 tatttttcca aatagtataa ttcaaaaata tttaggaaat ccaggtttgc tatctcttgg
    18361 aagtatagca atacttgcat gtgttatgta tgtatgtgct gtaggacata taccgtttat
    18421 agcagccctt gttgcaagtg gagcatctcc tggtatagcg ataacatttc taatagctgg
    18481 agcagctaca aatctaccag aacttataag tatgtttaaa cttattggaa aaagaactgt
    18541 tattatatat tctgtaactt tgacactgtc ttcaataata attggatata taacaaattt
    18601 acttttagtg aataataact cagtaaattt agatgcaaca aattcttcaa tacagttggc
    18661 aaataaacta atgttaaata tacctgatag ttttgagtat gtatgtacta ttttaattat
    18721 tttatttgca tttaaatcaa taataccaaa gatgaaagag gtcataggaa atcatgctta
    18781 aaaagatagt tataatatcc tgtatagtag ttgtgttaat cattttatca aaaatagttg
    18841 atgataacat caaagaagat gcttctattc caaatgtgaa taaagaaaca ttagaatatt
    18901 ttagaaaaaa ttataaagaa gacataataa cttgtgctga agaagactta aataatgatg
    18961 gaaaaaaaga tttagtagtt atatataaaa aaagcaataa ttcaaatgaa atggtagtcg
    19021 tagtttctga taaaaatagc cactatataa caaagccaat accagcacct atagagaatc
    19081 aaacgataac ctttaaaaat attgatgata aagcaccaat agaagtaatt gtatcagggt
    19141 ctaaaaatgg taatgtagga tatgctattt acagagtaga gggtaaaaaa tttgttgatt
    19201 tatttggcga agatatggac aaatgttgct aaaaataaaa ataatacata aaggtgggaa
    19261 atttatatga aattatctaa gaagattcaa gcattaatct tatttggtgt aatgggtaca
    19321 tctatattaa caggatgtag tagtaaacca aaagaaaaag aagaagcaaa aactggaaaa
    19381 gatgattata caataacagt aggttattat aattgtgacc atatgactgc gggaccagta
    19441 gcagaatcgg caggaatata taaagattta ggtcttaatg taaaaactgt tggaaatggt
    19501 aaagttcctg aagctatggc agcaggaaaa atggatgcag gttacatagg aactaagggg
    19561 ttagttggag caatacctaa gggttcgcca ataacaatag cagctaacaa ccatactggt
    19621 ggttctgaat atttaatagt ttcaaaagat ataaaagaac ctaaagattt aataggtaaa
    19681 aaaattgcaa cagatatgag tgatttctta tggacaagtg attatggtcc agaaacaggt
    19741 ctaccaactg acccatcaaa atatgaagtt gtaaatatgg attcagacaa agataaatat
    19801 ttagctctta aaactgggaa aattcaagct tttacatctt gtgacccttg gggttctgta
    19861 gctgaaaatg acggtgcagg aaaaataata gcatctactc aatataaaga aaaagctaat
    19921 ggcaaagaat ataactgttg ttcattctca ttaaacaaaa actttataaa agaacatcca
    19981 gatttagcaa aaaaattggt tctagcacat acaaaagcaa tagaatatat atatacaaat
    20041 ccagctgaag cagctaaaat atttgcaaaa tattataatg tagaagaaga agttgcactt
    20101 agaactatct ataagaagac tgttggagaa ggtagaactc ttacttggaa agttacaggt
    20161 gaagaatata aaaataattt acaaatgtat aaagatttaa aggctttaga tgatatacct
    20221 aaatatgaag atacgattga tacaagctta ttggactctt gtggagctga tgattttgac
    20281 aaatttataa aagaaaaagt taacccagag tttcctgaag gtatgagtta tgaagactgg
    20341 aaagcaaagg tattataaat aaatggagaa tatgaaaaat ataataatac tttttatcat
    20401 tattttggga agtatattta taatattttt taattttgaa gataaagaag aaataaaagg
    20461 aaaattaaga ataggggtat ctgatgatac ctctggtttt gtaataaatt atatggtaaa
    20521 tagagactac tttgaaaata taaaagtaag tgatataatg gatattttta caattaatga
    20581 ttgctgagca agtacaatgc agtgggctct gagttcagag gaaatagata tagcaatcat
    20641 atgtaaagat gctgcaaaac aatatgtaaa tgtaaatagt ggatttgaaa ttgttggtac
    20701 tgtagttcaa aattctgata tatttctatt aagtgataat aatcctgaaa agataggagt
    20761 cattcaaaat agagattacc agtcagaatt ggttaaaaaa tactacaaga atgtggaagt
    20821 agctccctta ttagggactg cactagcata tggattagaa agtaatttag tagatggtgt
    20881 tgtaatagat tgcataaagt caattgggtt agagggtaaa agaaaatcta caactaaatt
    20941 aggagattat gatacctatg tattagttgt aaataaagag tttaaaaaaa gtaaagcata
    21001 tagtgatttt ataacacttt acaataaaag tgttgatgct ttaaagaata aagctacatt
    21061 taagaaagta ttaaaagagt acaggaatat aagaaatata gatatggagg aaataagcaa
    21121 ttggaaatta aaatttctaa agataaaagc gatagattaa ctaagaaaaa aactgatggt
    21181 agtatggata ttttatataa aataatacta ttagttatta tatttttagt ctggcatttt
    21241 gcagcaaaag atatagggag ttctttgtta cttccaatgc cagtagatgt aataaaaggt
    21301 ttctttttct gtgtgacaga tgcagagaca gtcactaatc tttttataac attgcagaga
    21361 gtacttaaag gttttatgta tgcattattg tttgggcttc caataggatt tattatggga
    21421 ttttctaaaa cttttgaaag ggttttatct ccagtagtag attctgtaag gcaagttcca
    21481 ataatggcat gggttcctct tactatagta tggtttggta taggagatgg tccaacaata
    21541 tttttaatag ctttttcagg ggttttccct ataattttaa atactattca aggagtaaga
    21601 gcaatatcaa aggattatta taatgcagct agaagtatgg gagcatcgcc aattgtgata
    21661 tttacaaatg taattgttcc cgcatctctt ccagatatac taactggttc aaggattgct
    21721 ataagtacag gatggatgtc tgttatctga gccgagttta tagctacgag tgccggtctc
    21781 ggttactcaa tggtacaagc tcaaacatta atgcatacag atgtattaat tgggcttatg
    21841 atatttgcag ctttaatagg atttataata gatagggtat taaagtttat aaataaaatc
    21901 ctttgtagat ggaggtttgc agattaatga agttaagtgt aagagatata aataagacat
    21961 ttgtaaataa tagagtacat acaaaggttt tagaagatat aagtatggat atagatgatg
    22021 gacaatttgt ctgtctttta ggaccatcag gttgtggaaa aacaacttta cttactataa
    22081 taggtgggtt tcaaaaatca gaacgtggag atgtttttat aaatgataaa agagttaaaa
    22141 aaccgggcat agatagagct tttatatttc aaaattatgc tttatttcct tggaaaacta
    22201 taagaggtaa tgttctattt cctatgaaac agcagaaaat accaaaagaa aaaagagaag
    22261 aaatgctaga agaacttttg gttatgtcag acttgaaagg aaaagaaaaa ctgtttccgc
    22321 atcaattatc tggaggaatg aaacaaagag tggccatgat aagagcttta gcatgtaatc
    22381 cagaggtatt actaatggat gaaccattag gagctgtaga ttttcaaatg agacaaaatt
    22441 tacaggaaga gctagaacga atatggataa agaagaaaat aactgcatta atggtaactc
    22501 atgatgttga tgaagctgta tatatgagtg atagagttat agtaatgtcg agagataaag
    22561 gaagaattat tgatgatata aatatagata ttccaagacc tagaattaga ggaagccaaa
    22621 aatatgaaga gtacaaaaac aaattaactg atactttatc aaagtgttat gaagtttaaa
    22681 gaggagtaga catgagtcaa atagaaaaat acaatagttt ggataataca caacagaaaa
    22741 taagaatctc tattatggat atgataatag ataaaggttc tagtgttaca cttcaagaag
    22801 tgactgaata tgtaagtaaa aagttgaata tagaaaaaga atatatagaa aggacactac
    22861 aatattttat ttataaaaat ataatggttg ttgatgggaa tagtataaat tttatatatc
    22921 ctgtatcagc attgccaaca aatcataaag taactttgag agataataga agtttcagtg
    22981 caatgtgtgc tatagatgct ataggaacct cttgcacatt caaccaagat ataaaaataa
    23041 actctatttg tagtgctaca ggtaaggaaa tagaagtaat tataaaaaat gaaaagattg
    23101 agtatgtgaa taatccaaac ttaagagtgc ttcatataaa tcttgataag catctaaatt
    23161 gggctgctag ttgctgaaat ataatgaatt tcttctggac gaagagtgat tttgatgctt
    23221 ggacaaacga agcgggatta tctgatgatg aagatatata ttgtttagat attaatgagg
    23281 caatagtaga atcatataaa atatttaaac tcaaacaaaa ggtactatca tagatataca
    23341 tatatgaaaa actaaaaatg acctttagat aaaaatgata ggtaatatcc atactatata
    23401 aattagtata gagtagggat gtgaaaagta tttaaatcta aaggtatatt tttagttttt
    23461 ttagatgatt agatagcctt attaagtata tatttattta taacctttgc aactccattt
    23521 tcattattag tatcagtaac ataatctgca atttccttta tactattacg tgcattcccc
    23581 atagccacac caagacctgc atactttatc atatgttggt cattttcttc atcgccaaca
    23641 gcaatagcat tttctatagg tatatttaga ttcgtacata atgttttaag agcagttcct
    23701 ttattagtag ttttacctaa aaattctaga taaaaaggag cagaccttac tatagtatat
    23761 ttattgaata aatttttagg tagttgctgt atgatttctt ccaatctttc tggttcatca
    23821 actatcataa ttttacagat tttaatagat tcatcaattt tatctttttg tatatatttg
    23881 acttctatat tatttaattt cgcttcatga agtgtatatt tgccaggcgt ttcatttggt
    23941 gtaattaaat ttgtcattgt atttatgtgt attttagtct tatatttttt acaaacgtta
    24001 ttatatataa aatcaaaatc atctaaactc atttctacat catgtatgat tttctcactt
    24061 ttaacttctt gaataattgc tccattaaag catataacat aatcaccttc atctgttaaa
    24121 tttagttgat ttagaagttc ttttacacca ggaagaggtc tgcctgttgt tataactact
    24181 tttgccccag ccctttttgc ctcctgaatg gactcaaaga cttcaggagt aatccttttt
    24241 tgtgtattta atattgttcc atcaatgtct agtgcgatta atttgtacat atcaacctcc
    24301 aataaattat atactcaaat ttcctgtgaa aacgttgaca cagctagtgt tgaacaaagt
    24361 ttcagtggga aatatggttt gttttgtata ctattatatc aagagataag taatttagaa
    24421 agtttaataa tattaatttt ttgataaaaa tattacttat gtgagatgag aatgtaaaaa
    24481 ctttgtaatc aataattcca aaattcttaa ttacaaataa tggtatcgtt tgcattgtta
    24541 atataaataa atgtatatta tatagttaga agtttgaata aatagactga tggtaatttg
    24601 ttatattagt tagttttgtg ttataaaata ttgtaattcc attaatcata tgttattatt
    24661 caacatgatt ataatataaa gaatatttga tatatataat aatttataaa agagtattac
    24721 atagtacata taaatctata tttataaata caataaagta aagttgtaaa ggagtgcttt
    24781 aaatgaagtt ttcagagttt aagtatgaaa gacccaatta taatagtatg aaaaaagagt
    24841 ttttgagttg tgtagaagat ataaataatt ctcgtaatta taaagagcaa caaaaaaata
    24901 tacataagat taatttatta agaaataaaa tagaaacgtt gtcaaatata gcttcgataa
    24961 gatatagtac agacacattt aataaattct acaaagaaga aaaaaattat tgggatgaat
    25021 atatgccttt gtatgaagaa ttaaattcat atttttataa tgccattgta aattcaaaat
    25081 ttaaatatga tttgataaaa gagtttggag agcaattttt cacaatagta gagtattctt
    25141 taaaaagttt ttccaaagaa ataataagtg aattgcagga agaaaataaa ttgtgttcag
    25201 agtatactag acttttagct tcagctgaga taatgtttga tgggaaaata agaaatttat
    25261 ctggtatggg gaaattcatg tattctaaga atagaaaaac aagagaatta gcaaacaaag
    25321 catattataa tttctttgaa gaaaatgaaa ctaaatttga tgatatattt gataaattgg
    25381 ttaaacttag agataagatg tctaaaaaat taggttttga agattttgta gagcttgggt
    25441 acattagaat gatgagaagt gactacagag aatatatgat aaaaaatgtt agaaaacaag
    25501 tactaaaata tgttgttcca atggcaaatg agctttatga aaaacaagct aaaagaattg
    25561 gtctagaata tttaagttac atagatgaag gcgtggaatt tttaacagga aatgcaagtt
    25621 taaagggaga ttcaagatac ataattaaaa atggtaaaag gatgtattct gaactgtcaa
    25681 aagaaacaaa tgaatttttt gattttatgt tagaaaatga acttatggat ttagaaacga
    25741 agaaaggaaa aggagctgga gggtattgta cttatatacc agattataag tcaccattca
    25801 ttttttctaa ttttaatcaa actgctgatg atatagatgt attaacacat gaggcaggac
    25861 atgcatttca gttgtatatg tctagatgga ttgatatgcc agaaattaat tttccaacgc
    25921 ttgatagttg tgaaatccat tcaatgagta tggaatttat aacatggccg tggatggatt
    25981 tattttttaa agaagataca gataagtaca aattcactca cttatcatct tcaattaaat
    26041 ttatacctta tggagtaatt gttgatgaat ttcaacacta tatttacaaa aatcctaatg
    26101 tagataagtc aaaacgaaaa gaaatctgga gatttttaga gaaaaaatac ttacctcata
    26161 gaaaatatgg tgataattct tttttagaga gaggttgttg gtggtttaaa caaggacata
    26221 tatttaaaaa tccgttttat tatatagatt atgtattagc tcaaatttgt gcgttacaat
    26281 tttggaaaaa aatgattcaa gatagagatg caggatggaa agattatata aatatctgta
    26341 aagttggagg aacaaaatca tttttagata ttgtgagtat gggaaatttg tattctccat
    26401 ttgatgatgg ctgtattgag tctattatag gtgatgttaa atcttggttt gatgaaataa
    26461 atgacagcaa attataaaaa attatgagaa ttttattgta taaaagtaaa aaattaaaaa
    26521 atatataaaa agtgattatt tgttgtgaaa atagtcttgt ttttaactaa attgataaaa
    26581 taaactaaaa aagatttgta cttaaaaaaa aaagaaaata caagaaatat gcaatatcaa
    26641 catttggaaa aaaaagtaaa aatgtataca aaatacatat aaaaaaacta ttgcaaattt
    26701 tatagaatgt ttatatactt aagtcaacag agatagaaaa aagctattta tagaaaacaa
    26761 aggataaatt gcttaatttt aaattttctg aaaaaaataa taatccggat gaagagtatg
    26821 ggagactgct ttataaaaag cgaccgaagg gacaagttta atcttactta cccaaaagat
    26881 tagatgaaat tctcaggtaa aggtgactgt acttggacgg ggctctgaaa atgattttaa
    26941 cagggaagac aaattgggag atttgttttt cctgtttttt atttatcaag taataaataa
    27001 aagtttgaaa gagagtaaaa aaatgataat aattacgaat aatccaaaag taaaagaaga
    27061 agttcaaggc agagaagttt tgtttaaaga cacaacttat attggaatct tagaagcaag
    27121 tagagatttg atacatgaag gttatgaact tttatctcat ccactctatg ggagtgtaaa
    27181 gccaaatgaa acaccttaca ggactgttat actaaagaaa ggaaatcgtt tggatataaa
    27241 ctctttaacc ttaattgaag aagcaatcat cactgcaagt aaatttcaga ataataaaaa
    27301 gactccaaaa tggacagaaa gtgttcaaga tgattttaga gtaattgatt atgatatatt
    27361 ttataataca atacaaagaa tgcagtatga ataaaaaaat tgttaaaaag ttattaaaac
    27421 agcctaaata ttaaatctaa tttaatatta aataaaatga gaaatttaat tggggaggat
    27481 aagtcaaaat ggaaaatgtg tatgacttag taataattgg ttcaggacca gcagggcttg
    27541 cagcaggtct ttatggagca agagcaaagt taaaaactct aatcctagaa aaagacaaga
    27601 ctggtggaca aattgtaata acacatgaaa ttgcaaatta tccagggtca gttccaaatg
    27661 caacaggacc aagtttaata gctagaatgg ttgaacagtg taaagagttt ggagcagaaa
    27721 tgctaagaga caacattgtt gatactgagc tagatggaga tataaaagtt ttaaaagggg
    27781 aaaaggcaga atatagagca aaagcagtaa taataggaac aggagcaaca ccaagaaaga
    27841 ttggttgtcc tggagaaaaa gaacttactg gtaaaggggt ttcatattgt gcaacttgtg
    27901 atgcagattt ctttgaagat tttgaagtat ttgttgtagg tggaggagac agtgcattag
    27961 aagaagcaat gtatttaact aaatttgcta gaaaagtaac aattgtacat agaagacaag
    28021 gatttagatg tgctaagagt gttgaagaaa aagcaaaagc taatccaaag atagagtttt
    28081 tattagatac agtaattgaa gaaatcaaag gtgatggaat attagaatca gttgtattta
    28141 aaaataaagt tactggagaa actcatgaat actttgcaga tgaagaagat ggaacaatgg
    28201 gagtatttgt gttcgttgga ttagatgctc aaactgattt attcaaaggt aaagttgata
    28261 tggatgaaaa aggttacata ataactgatg aagatatgag aactaacata ccaggcgtat
    28321 ttgcagcagg agattgtaga tctaagactc taagacaggt tgtaactgca actaatgatg
    28381 gagcaattgc atctatagta gctgagaaat acattgatga aaaatttggg aactagaatc
    28441 tgtaaattaa gaaatagtgt ctgtaaattt aatgaataat atttaaaatt aaaattaata
    28501 tttaaaataa aaataaaaat ttcaaggggg aataagaaaa tgttagattt agataaggct
    28561 acatttgaag aagaagtttt aaatgcagaa ggttttgtat ttgttgattt ttggagtgaa
    28621 ggttgtgagc cttgtaaagc tttaatgcca gatgttcata aattagctga gacttatggg
    28681 gataaaataa aattctgtaa gatggataca acaaaagcta gaagattagc tataaaacaa
    28741 aaagtattag gtcttccaac tatggctata tacaaagatg gagaaaaagt agacgaagtt
    28801 actaaggatg atgctacagt tcctaatata gaaaacatga ttaaaaaata tctttaatat
    28861 gttgataaa
//
LOCUS       DAYASV010000037        28740 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYASV010000037 DAYASV010000000
VERSION     DAYASV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.037"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            138..488
                     /locus_tag="ACGI68_001628"
     CDS             138..488
                     /locus_tag="ACGI68_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFH0138070.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            507..2150
                     /locus_tag="ACGI68_001629"
     CDS             507..2150
                     /locus_tag="ACGI68_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFH0138071.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2231..4288
                     /gene="recG"
                     /locus_tag="ACGI68_001630"
     CDS             2231..4288
                     /gene="recG"
                     /locus_tag="ACGI68_001630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFH0138072.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4347..4901
                     /gene="rsmD"
                     /locus_tag="ACGI68_001631"
     CDS             4347..4901
                     /gene="rsmD"
                     /locus_tag="ACGI68_001631"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFH0138073.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4903..5400
                     /gene="coaD"
                     /locus_tag="ACGI68_001632"
     CDS             4903..5400
                     /gene="coaD"
                     /locus_tag="ACGI68_001632"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFH0138074.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5404..5928
                     /locus_tag="ACGI68_001633"
     CDS             5404..5928
                     /locus_tag="ACGI68_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138075.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6272..8191
                     /locus_tag="ACGI68_001634"
     CDS             6272..8191
                     /locus_tag="ACGI68_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138076.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENIL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKSVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSILELINKQN"
     gene            8204..8491
                     /locus_tag="ACGI68_001635"
     CDS             8204..8491
                     /locus_tag="ACGI68_001635"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138077.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            8624..9883
                     /locus_tag="ACGI68_001636"
     CDS             8624..9883
                     /locus_tag="ACGI68_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFH0138078.1"
                     /translation="MLDFISNNILRNPSMLIGLIAAFGLVLQKKKASDVIKGSLLAAF
                     GIIIMETGTGMLVSSIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAI
                     HLMIARFTPIKTIFLTGHMLYWFPFIFVASGVEAGLNGGVLIAFAAVLSALYWSIMPW
                     LLRKYVFDVIGDDSFTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREIS
                     ISGGMAVFLMFIVVGIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQI
                     MPAFQGISEKVVPNALPAFDCPILFNYKPNAVLMGFLVAMLTSTAMILICNYFNVFNL
                     LLIPLVITSFFECGAAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNW
                     ILIFGGNDLSLFGIISRFIGSLFGGIL"
     gene            9886..10626
                     /locus_tag="ACGI68_001637"
     CDS             9886..10626
                     /locus_tag="ACGI68_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0138079.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            10949..12259
                     /locus_tag="ACGI68_001638"
     CDS             10949..12259
                     /locus_tag="ACGI68_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFH0138080.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAAKFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLISS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            12357..13274
                     /locus_tag="ACGI68_001639"
     CDS             12357..13274
                     /locus_tag="ACGI68_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0138081.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            13290..14099
                     /locus_tag="ACGI68_001640"
     CDS             13290..14099
                     /locus_tag="ACGI68_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFH0138082.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            14157..15905
                     /locus_tag="ACGI68_001641"
     CDS             14157..15905
                     /locus_tag="ACGI68_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HFH0138083.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYIGIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKVREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     RYDKVKKYVLELLDRV"
     gene            16209..17189
                     /locus_tag="ACGI68_001642"
     CDS             16209..17189
                     /locus_tag="ACGI68_001642"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HFH0138084.1"
                     /translation="MFFLSISFIGFYLVIRDNSDTVKMQRGINIGNALESPKDFPWDV
                     KMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKIDKYVDYALDKDLIV
                     VLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELLNEPKENLSSQLLNE
                     YIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAFQG
                     NIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIFLGEFGITKEAPEVS
                     RRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILSALIEKR"
     gene            17194..19500
                     /locus_tag="ACGI68_001643"
     CDS             17194..19500
                     /locus_tag="ACGI68_001643"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0138085.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMRILAEKMGVNYITRTEREGAKAGNLNNAMQHTTS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTILVSKPSEKKEKSIDEFEPMEYI"
     gene            19511..20587
                     /locus_tag="ACGI68_001644"
     CDS             19511..20587
                     /locus_tag="ACGI68_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138086.1"
                     /translation="MKTLKVFFCSTKYLILVFIFFINTTFISSATSDKILDLSFKKIE
                     TDLNSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNFYIEIQNKNKSSQR
                     INLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEFEGKIYVNFNSLIN
                     EESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKLRFLNNIKIIGDEE
                     VQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQKGKLTLSNKAKPGQIILQV
                     NVDDKFKIGKKITLTESWSVNKKDKDGVPYTLVYPEQSPTVQDMKKINFMNNIITFVR
                     ILFVSLVIICFGIYLYWKKCSKTK"
     gene            20605..22023
                     /locus_tag="ACGI68_001645"
     CDS             20605..22023
                     /locus_tag="ACGI68_001645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HFH0138087.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPSIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            22038..23246
                     /locus_tag="ACGI68_001646"
     CDS             22038..23246
                     /locus_tag="ACGI68_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138088.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(23320..24708)
                     /locus_tag="ACGI68_001647"
     CDS             complement(23320..24708)
                     /locus_tag="ACGI68_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HFH0138089.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVIWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IGFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            25049..26383
                     /locus_tag="ACGI68_001648"
     CDS             25049..26383
                     /locus_tag="ACGI68_001648"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HFH0138090.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREISYQGSLSSSCIKIMD
                     MEAASTGITERQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(26525..27442)
                     /locus_tag="ACGI68_001649"
     CDS             complement(26525..27442)
                     /locus_tag="ACGI68_001649"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFH0138091.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            27650..28534
                     /locus_tag="ACGI68_001650"
     CDS             27650..28534
                     /locus_tag="ACGI68_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HFH0138092.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEEKKVVEGSKKTQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
BASE COUNT        11236 a         3070 c         5103 g         9331 t
ORIGIN      
        1 ccaaaaactt ttctaggttt aaattatacc atatttatag tatgatatga ttataatttt
       61 atataataaa taagtaaaaa tagtaaaaaa taaattaaga aatgttttta ataaaaataa
      121 gcttaagggg gtcaaccatg agtgcaaagg taacgaatca atatggaaat atagaaatag
      181 ataatcaagt aatagctcaa gtcgtatata gagctgcaat ggaaagttat ggcttagtag
      241 gtttagcata taagtctaaa ggaatagtag agctattaaa aggcgagaac gcaactaaag
      301 gtgtaagagt tgaggaagta gaagaagatg ccatagcaat cgaactttat gttataatac
      361 aatatggtac aaatatatct actgttgcca ataatataat agatagagta aaatatgtag
      421 ttgaaaaaat gactggcgta agagttacta gaattgatat taatgtacaa ggaataagag
      481 ttaagtaatt tcataggagg aaacaaatga ttcaatatat agatggtaaa aggttaaggg
      541 agatgtttat ttcaggtgca aacaatctcc aaaacaataa agagttagtt gacaagttaa
      601 atgtattccc tgtaccagat ggagatacag gaactaatat gtcattaaca atatcttatg
      661 ccttaaaaga attagcaaaa gttgaaaatg ataatatatc agatatcgga aaagcattat
      721 ctaaaggttc tttaatggga gctagaggaa actcaggagt tatactttca caaataatca
      781 gaggtatagc taaatctata gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag
      841 cttttaaaaa tggttcagac acagcatata aagctgtaat aaaaccaatt gaaggaacta
      901 tacttactgt agttagagaa agtggagagt ttgcaataaa aactgcgaaa aaagagaaag
      961 atgtagtaaa gtttttaagt atgcttgtga aggaatcaaa ttcttcatta gaaagaactc
     1021 cagaccttct aaagaatcta aaagaagctg gtgtggttga ctctggtgga aagggtcttg
     1081 tacttatata tgaaggaatg ttagcttcta taaaaggcga taacatagaa ataaaaaatg
     1141 ttgacctaga tactaatatt tcaacaagta tggattttgc aaagagtact actagcacag
     1201 atgatataaa atattgctat tgtacagaat ttattttaga aagttctaag gtagaagata
     1261 ctaaaataag agacataatg atggcttatg gtgatagcct agcagtagtt ggagatgatg
     1321 gagtcataaa ggtacatgta cacactaatg acccaggaaa tgtacttcaa gaagctctaa
     1381 agtacggtca attattaact ataaaaatag aaaatatgaa gttacaacat gaaaatacat
     1441 tactggatgt agaagaaaag aaagaaaatg atagtgaacc tttagaagaa gaaaaagagt
     1501 ttgggtttat agctacttct atgggtgaag gtttggcaaa tatatttaaa gatttcggag
     1561 ttgaccatat aatagaaggt ggtcaaacta tgaatccaag tactgaagat tttatgaatg
     1621 ctataaagca tataaatgct aaaaatatat ttattttccc aaataacagc aatataataa
     1681 tggctgctaa tcaggcaaaa gaattgagtg ataaaaatat aatagttata cctacaaaaa
     1741 atacgcctca aggatttgct gcattagtaa cttttaatgg agaattaagc gaagatgaaa
     1801 ataaagaagc tatgatgaat gctttaaact cagttaaatc aggtcaagta acttttgctg
     1861 ttagggatac agtgatgaat gaaattgatg ttaaagaagg taatataatt ggaattgcag
     1921 aaggaaattt attatcagca ggagattatg tggatgaagt aacatcaaac cttattgaaa
     1981 agttggttga tgaagatact gctataataa cattattctt tggtgaagat gttacagaat
     2041 ctcaagcaaa tgaactacgt acttctttag aagaaaagtt tgaagatgta gatgtagagt
     2101 tatattatgg aggacaacct ctttattatt acttaatttc agttgaataa tgattataag
     2161 aacctgtatg accaagtagc ttttataaaa ttggttgtac aggatttttg tgtagtaggt
     2221 gatatatttg ttggatttat ataaagatgt tcaatatgtt aagggaatag gacctaaaaa
     2281 agcagataaa ttaaataaac tgggtatatt tacactaaaa gacctattgt attattttcc
     2341 aagacaattt gaagatagaa ataatctaaa gaaaatagca caattagaag atggtgagaa
     2401 agttacgata aaagcagtaa tatcaagtat aaatacattt agcccaaaag aaggtatgac
     2461 tttaacaaag attgatgtaa aagatgagac aggctcggct aaattagtgt tttttaataa
     2521 gagttatata aaaaatactt ttcgtccagg agattcaata ttagtttttg ggaaggttaa
     2581 gaaaaaattt aataacttag aacttacatc atgtgaatta gaatatctta ctaattcacc
     2641 taagaataca tgtagattta tgcctgtgta ccagcttaca tacggtgtta caaataaaga
     2701 aattatgagt ataattagaa cagtacttga agataaagaa ttaattatac aagaatatat
     2761 gccacaaaga attatagaaa aatataggct atgtagtata gattttgcag ttagaaacat
     2821 acattcacca agtagtaagg aatcattaaa aatagcttta tatagaattg tatttgaaga
     2881 attacttata ctacaactag ggttgtttgt atttaaaagt ggcagaaata aagaagatgg
     2941 aataaagttt gaaacaagta aagacttaaa aaaaataata agtgctttac catttaaact
     3001 gactaaagct caaaacaggg cattagatga aattatacaa gatatgaatt tagagaaaat
     3061 aatgaataga ttggtacaag gtgatgtagg gagtggtaaa acagtagtag ctctcttagc
     3121 acttgcaaat tgtgttttaa atggttatca gggcgctttg atggcaccta cagagatatt
     3181 agcagggcaa cattatattt cacttactga gtctctaaaa gattttggaa taaatgtagg
     3241 tctattaata ggtagtctta caaaaaaaca aaaggataca gtattggaac aaataaagaa
     3301 taatgagatt gatatattaa taggtacaca tgcactaatt gaagataagg ttgaatttaa
     3361 taatattggt ttagttataa cagatgaaca acatagattt ggtgttatgc aaaggagcaa
     3421 actttcattg aaaggagcaa atcctgatat attagtaatg acagcaacac caataccaag
     3481 aactttagca cttatactct atggagattt agatatttct attatagatg aacttcctcc
     3541 aggaagacag ccaatagaaa caattgctat tgaaaaaagt aagcgagata gagcatataa
     3601 taatcttgta agaagagagg ttgaatcagg aagacaagtg tacattgtat gtcccttggt
     3661 agaagaaagt gaagccatag aagccaaatc agcagttgaa cttgttgagg agttaagagc
     3721 ggaatatttt cacgacttaa ggcttgggtt gcttcatggt aaaatgaagt caagtgaaaa
     3781 agatgaagta atgagacttt ttaaagataa agaaatagat attttagttt caacaacagt
     3841 aatagaagtt ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa atgcagaaag
     3901 atttggactt gcacaattac atcagcttag agggcgagtt ggaagaggta gtcataaatc
     3961 gtattgtgta ctaatttatg attctaaaac agatgtatgt agacaaagaa tggctataat
     4021 ggaagagacc aatgatggtt ttaagatttc agagaaggat ttagaaataa gaggacctgg
     4081 ggaatttttt gggacaaggc agcatggtct tccagaactt aaggtagcaa acttatttaa
     4141 gcatataaaa atactgaagt tagcacagca agaagctcga tatatattgg gtgaagataa
     4201 taatctacaa ttaaaagaaa acatggcact caaaaaagaa attatagata aatttaaaga
     4261 tacattgaaa gaaatttcat taaattaatt ttaaattgtg ttaaaatatt actaatgtaa
     4321 taatgtgctg aagggagatt gtcatattga gagtaatttc aggaaaagcg agaggattaa
     4381 aactaaatac tccaaaaaat gaagatgtaa ggccaactac agatagagta aaagagtctc
     4441 tatttaatat gataaattca tatataatgg aaagtgaagt tctggattta tttgctggga
     4501 ctggttcttt aggaatagag tgcttatcta ggggtgccaa gtcatgtact tttgtagata
     4561 ttagtaagga aagcatagat attgttaagt ccaatataaa aaaggcaaga gttgaaagtg
     4621 aaagtattat tttaaacctt gattttaaga ctgcaataga taaacttaaa ttacaaaata
     4681 gtaaatttga cataattttt atggaccctc cgtattataa aaatatgttt atagaagcta
     4741 ttgaaaaaat tgataattct aatctattga acgaagatgg aataatagtt gtagaacatg
     4801 atacgaatga tttatttcca gataagatat gtaaattgga gaaaactaga gataagaaat
     4861 atggaaatac tacattaact ttttataaga tggaggcata atatggaaaa taaacctaga
     4921 aaagctatat ttgcaggaag ttttgaccca attactaatg gacatttaga tattatttgc
     4981 agggcttcga agttatttga cgaattacaa ataggagtat taaataatcc taataaaaag
     5041 ggtctattta gctttgatga aagagtaaaa cttatagaaa aaagtacaag ccatttaaat
     5101 aatataaaag ttgtgacttt tgatggttta ttaataaatt actgtcagga aaatgggatt
     5161 ggagcattag ttagaggtgt gagaagtggt gctgatgttg attatgaact tcagatggca
     5221 catatgaata gagagcttaa tccagatata gagactataa ttttaccaag ttgtacaaaa
     5281 tattcattta taagttcatc tttaataaaa gaggttttgc tctttgatgc tgatataaaa
     5341 aatttggttc caaagattgt tttggaagaa ttaaagaaaa aaactagtgg gggtaattga
     5401 tatatgaaga tagatttaga aatgattgaa ctatttgagc agttagagga gatatttaga
     5461 agtgcttcta caatcccttt ctcacataaa tgcacagtgg ataaagatga ggttttagca
     5521 ataattaatg atataagaac tttaatacca gaagaagtaa cacaggcagt atggattaat
     5581 aaagaaagaa ataaaataat aagtcaagct aagcaagatg ctacaaatat agttgaacaa
     5641 gcgaaaaaag aagctgagag aatacaacaa gaatatcaag ataatataga ggaattaaag
     5701 aaaaattctg aagatgtagt aaaggcatat gtagaatcca gtgaacctgt tgttcaagca
     5761 gaccaaagag caaaagatat agttgataga gctgagagaa tagccaatga gataagaatt
     5821 gggtcaattg aatatgcaga ggatgtgctt tctagtgttg agtataattt aagagaaata
     5881 ttagaagaag taaaaagaga taaagctgaa cttagaccta aaaaataaat tttaaataaa
     5941 taaaaatgga gctatcacac aaaaaattcc aatttatgtg tgatagctct cttttaatat
     6001 agcaattttt tgttatataa ttgtgctttt tagattactt atatcttatt ttgatattat
     6061 aatgtagttt aatcttttat ttgttgaaat caacttattt atttgtataa ataatatagg
     6121 ttattttcaa atttttttaa taattttacc actgtcttct tttagattag agttagactg
     6181 tgcaaaatta taaaataata ctaaatcatg tgcaattatt tggtatggca ctacatgtag
     6241 aaaatataga taataaaatt aaggaggtag tatgaaggaa agggctaaaa aaatattaga
     6301 ttttattatt aaaaataaca atgtaacatc acaagaacta caagaaaagt ttaatgtttc
     6361 aaagagaact atttattacg atattctagc tataaatgaa caactaggaa aaagtggtaa
     6421 tataaaaaat gtaaaacaca aatttatatt tgaaggtaat ttatgtgatg caaggaaaat
     6481 aatttcaact gaagaagata aatttttaga tagtgattac agaaaaactt ttatattaaa
     6541 caagatatta cttggcgaaa aaatatcaat agaaaaacta acaaatgaaa tgttactttc
     6601 aaaaaacaca gtagtacaaa caataactga tgtaaaaaaa taccttcaaa caatggggtt
     6661 aagattagag tataagggaa agtacaaaat tataggtgat gaatacgtta ttagagagct
     6721 gtttctgata attgttcaag aaaatatatt ggaaattaat tctataagtg aggaagtaag
     6781 tagtttcgat acaaaaggac atataaaact tactgattat tctttattaa atctaactaa
     6841 gtttgtggaa tttctaaata aaaggataag agatgggaaa acattatact catataaata
     6901 tctaaatgaa gctaaaaaaa ttagttactt tagtaattgt aaagaacttt tatgtgaaga
     6961 agctaatgaa aatgaacaag cctatatatg cacttacata agttcacttc ccagcttaaa
     7021 tagtgaagtt aaggaagatg ttgttgaaga atatgtagat aaacttatag ataagtttga
     7081 agtaaataca gcaataaaat tagaaagtaa gcatgagttt aagaaaaata ttttaaggca
     7141 cctccattct tcttacaaca gaatacgatt taagtttcct attagaaatc caatgttaga
     7201 tgagactaag tataaacatg agtcacttta taaaattata aaatcaatta tagaaaatga
     7261 agaagagttt cctgtatttg aggggataag agaagaagaa ataggattta tagcagcgta
     7321 ttttggagga tatttaagag ggtctaggga taatggtcta agaagaaata aagtcttact
     7381 agtttgtcct aatggtctta tggtatcaaa gagtttggaa atacaactat ataaatacat
     7441 accaaccata gaaatagtag gtatagtatc cattaagcaa ctaaaagaag taaatgtata
     7501 ctacgattac ataattacaa ctatagatat acaaaatgta aacaacgtaa ttgttgtaaa
     7561 tcctctttta acatcatcag atgttcaatt acttatgaac aaactaataa gtgtaaaaga
     7621 aaacgagaag tatttcaatc ttgaattaat aatacaagct ataagaaaaa atggagttat
     7681 caataatgaa gaagcactaa aagctgattt attaaacata atacacaaga tagatgaagg
     7741 agagatgtat caaccaatgt taaaggaatt aattaatgca gaaagggtca atataataaa
     7801 aagtgttaga gattggaaag aagcaataaa aatagcctca aagccattgt tagaagataa
     7861 ctctatagaa gagttatata tagaaaatat gataaagtca gttgagaaat atggaccata
     7921 tattgtttta gctgatagat ttgctctacc acatgcaagt tctaaagaag gagtaaacaa
     7981 gcttgctatg tctttactaa tagtagaaga tgaagttgat ttattaggaa aaccagtaaa
     8041 tatatttatg gtattagctg ctgtagataa cactactcac attagagcat tagcatcact
     8101 atcagagatg atgtacgagg aagaaaatgt aaaactaata attaatggtg ataaaagttc
     8161 aatactagaa cttataaata agcaaaatta ggaggaatga aaaatgaaga tattagcagt
     8221 atgtggtttt ggtgtaggtt catcaatggt attaaaaatg acaatacaaa aggctctaaa
     8281 gcagttaggt atagaatgtg aagtagaaaa tacagatata aattcagcta gaggtgctga
     8341 ttgtgactgt atattcacaa gtcaagaatt gttaaatgaa ttaaaaggaa cttgtaatgt
     8401 agatgtatat ccagttaaaa agtacatgga tttagcagaa gtcacagaag ttgttcaaac
     8461 ttttttaagt gataaaaatg ataagaaata gtataagttt taaaaagtaa atttaagtta
     8521 atcagaataa aataaaaact tactaaattc aaaaatagta agaaagagag gagggattta
     8581 gtggtagaaa acttaaacag ttgacctatc actaaaaaat ataatgcttg attttatttc
     8641 aaataatatt ttacgtaatc catctatgtt aattggatta atagcagcat ttggtcttgt
     8701 actacagaag aagaaagcat ctgatgttat aaaggggtca ctacttgcag ctttcggaat
     8761 tataattatg gagactggaa caggtatgtt agttagttca atagcaccaa ttaatggagc
     8821 tttccaaagt ataagtggtg gtgcagtaac aaaaggttta agtgatgtta cttttactgc
     8881 aagttatggt ggaattatag gtttagcaat gtttgcgggt ttagctattc acctaatgat
     8941 agcaagattt actcctatta agacaatctt tttaacaggt cacatgcttt attggttccc
     9001 attcatattt gtagcttcag gagttgaagc tggattaaat ggtggagtat taatagcttt
     9061 tgcagcagta ttatcagcac tctattggtc aataatgcct tggttattaa gaaaatatgt
     9121 gtttgatgtt ataggagatg attcttttac attaggtcat ccatctggtt gtttagcact
     9181 tatatcagga tttgttgcta aattagtagg aaataaagaa aaatctacag aagatataaa
     9241 catacctcaa agtttatcat tttttagaga aatatctata agtggtggaa tggcagtgtt
     9301 tttaatgttt atagttgttg ggatagtaat accaggtgcg tttaaagata gtaatgagtt
     9361 tattggaacg tatgcaataa agcaaggtct ttcttttgga gcaggtctta taataatgct
     9421 tcatggggta agaatgctaa taaatcaaat aatgccagca tttcaaggta tatctgaaaa
     9481 agttgtgcca aatgcactac ctgcttttga ctgtccaata ttatttaatt ataaaccaaa
     9541 tgctgtatta atgggattct tagttgctat gctaacatct acagctatga ttttaatatg
     9601 taactacttt aatgtattta atttgctact tataccactt gttataactt cattctttga
     9661 gtgtggagca gcagcagtaa taggagaatc acaaggtggt atacgtggag ctataatagg
     9721 tacagctata gctgcaatat ttatggtagt tttagtagga atatctgctg taatttactc
     9781 aggaactatt caaaattgga tactaatatt tggaggtaat gacctttcat tatttggtat
     9841 aatcagtagg tttataggtt cactatttgg aggaatatta tagatatgaa atttaaatac
     9901 atagatgaga taagaagatt aattgatata atagaaaacg aagaaaaagt caatatggaa
     9961 aaagcagtac aaatctttgt tgaagcaata gaaagtaaaa atgctatatt ttcatttgga
    10021 gcttcacatg caggaatatt aacagaagaa ttattctata gagcaggtgg ctgtgttctt
    10081 ataaatccag tatttgcaaa atctctaatg ttagatacag cgcctataac tcacacaagt
    10141 aagatggaaa gattagaagg atatggtaca gctctagctg ataaaactcc aattaaaaaa
    10201 ggagatgtag ttatagttca ttcagtatct ggtagaaatc cagtaagtat agagtttgct
    10261 atgaaagcta aggaaaataa ggcaatagta atatgtataa ctaatttaag ttactcaaaa
    10321 agtgtatctt caagacattc ttcaggaaaa aaactttatg aagtatgcga tttagtgata
    10381 gataatcatg gtcaaaaggg agatgcctgt gtagagatag atggattaaa ccaaaaagtt
    10441 agtccaactt ctactgtaat agcaactact gtgatgaact ctattatagc tcaagctact
    10501 caggaattag ttaataaagg gctaaagaat cctccgattt tctatagtgc aaatattgat
    10561 ggtggagatg agttaaataa gaagattttt gatgaatata aaagtgtaat tcattatgaa
    10621 tattaaggtt aaaaaaatat gttaatgaac tttgaagttt atcaacaaag aatattaata
    10681 atatataata aatgctatca tttaaaagct gtaggtaaaa atctacagct ttttattatt
    10741 gtagattaaa aagatgcgta attaaatttt aaattggaga aaataaattg gaaggtatca
    10801 attataaata ttagaaacgt tgtcatagaa aaaaactata ttaaaaaaaa gtacatagaa
    10861 aaaactgatt agacaataaa tacatgcttt gataatattt taaatggagt gaaaatgtgt
    10921 agaattataa tgaaaaaaga aaataaaaat gaaaaaagaa aataaaatgg agggaataaa
    10981 agtgggtatg ttaaaaaaat tatgttcact agctatggta gcaattatga ctacaacact
    11041 tataacaggg tgttcaagtg gaaatggaaa agattcaaaa aaggatggcg aaaaagaaaa
    11101 actagttctt tggatgccac cagtagaaaa taatatgaag gaagtttggg accctatttt
    11161 agataaattt gaggaaaaaa ataactgtga agtagattta cagataattc cttgggataa
    11221 ttattctgaa aaatttgcaa cagctataag tgctggcgaa ggtccagatg taggatatat
    11281 gtatgcagaa atgtatccac agtttataga aagtggagca gtagaagatt tgactaacta
    11341 tgcaactaaa gaggataaag agcaatctat ttatataaaa acatctgaaa tgatgggtgg
    11401 aatgtatggt atgccattcc aagcagcaaa tccaggggta ttatactata acaaggatat
    11461 tttagagaaa ctaggagaaa aacctcctaa aacatgggaa gattttaaaa gaatttgtaa
    11521 aaaagcaacg caagatactg atggtgatgg aaagatagac caatggggat tagctcaggg
    11581 atggggagct aaaacttttg gtaacttaaa ctggaactgg tatccttatt tatggcaagc
    11641 tggtggagat atatttaatg atgatttaaa atcggttaaa tttaatgata agagtggttt
    11701 agaagcagct aagttcttaa aagaattaca agcatatgtg cctgaagatt ctttatctaa
    11761 agatagtaat gaaatgatag aaagtgtatt tggaccaggg aaagcagcat ttacaataat
    11821 gttatcttct gctgcaacaa gtgtatttga tgaatcattc ccggatttaa attggggatt
    11881 tgtaactgga ctagaagata agaaagcagc aacttttgga gcagtagacc atttatcttt
    11941 aatctcatct gcgaaaaata aagaacttgc atacaagtta atacaacata tgttaagtgt
    12001 tgaatcaatg acagaatttc ataaagcaat accaagagca ccaataacaa aaggtgaacc
    12061 atatcaaggt gatgaaagat tcaaagaaat ggtggaaaat gacaaaaatg tatatagacc
    12121 attagtagta ggacctcatg gagttgaaat atatgaatat ttatggaaag aacttcaaac
    12181 tatgatttca ggagataaaa ctccaaaaca agcgttagat gatgctgcaa aatattcaaa
    12241 tgatttatta gcacaataaa agtttaaagc tgtaatatgt tgtaaagtac tttacagatt
    12301 aaaattagca ttgtatttat aaatacaatg ctaatattta tattggagag gataaaatgg
    12361 aaaacttagt tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa ttaaatatta
    12421 aaccatatat gtatatattg ccattgggaa taattttagt ggcgttttat gtgataccaa
    12481 taataatgtc aatatacttt agttttacaa agtataatat tattagtcca gctactttta
    12541 taggtttaga aaattataaa aaattattta cagatgaaac attaaaagta tctattataa
    12601 atactattaa atttactgtt gttgtagtac cttgccaaac tatattatca ttgattttgg
    12661 ctgtttggat tacaggaaaa ggaaatagta aaatagcttc atttgctaaa ggtgctatat
    12721 ttataccagt attatcatct atggttttga taggtatggt atggagggct ttattgaatg
    12781 gagaaggttc tattatatat caggtattag gtgcctttgg aatagaatca tctaaacttt
    12841 taggagatag taagacagca ttacctacac ttatgtttat atctatgtgg aaaaatatag
    12901 ggtatttcat ggtaatttat atttcagcta taatgaattt accaaaacac tgctatgaag
    12961 tagcgaaggt tgatggggct acaaaacttc aagaatttat aaaaataact gtacctttat
    13021 tgaaaccaac tacaataatg gttgtatttt tagggtcgat atggtcatta caagtatttg
    13081 acttaattta tactgtgaca ggtggaggtc caggtatatc tactatgagt atagttatgc
    13141 atgcatttaa cttaaacttt aaaaacttta attctggata tgcaatgact gtagcaaacg
    13201 tattattttt gttaatagca gtagtttcga ttttacaaaa taagcttgta aaacgagaca
    13261 attcagattt ttagaaaggt aggtataata tgaaaacagc aagaagatta ttaagtgatg
    13321 gtttactttt atttatagcg tgtgcaagtt tggtaccttt tatatacatg ttgataatat
    13381 cattgaagat aacatataac tcatatagtt tggatatatc tttttcaaca gtaacccttc
    13441 aaaactatat agatattttt actaaaaaag gatttgcaca atattttttt aatacagcaa
    13501 tagtatcttt ttcaggtgta ttattaaatt tagtatttag tacattagca ggatattctt
    13561 ttgcgaagat ggattttaaa ggtagtgata agttattttt atttatgata atgactttaa
    13621 ttataccttc ccaagttaca atgataccac tatatataat aatgaaacac ttaggatgga
    13681 taaatagtta tcttgcactt attatgccaa taccaactgc atttggcgta tttataatga
    13741 gacaagctat tttaggagta ccaaaagagt tattagaatc agcaaagata gatggttgtt
    13801 cggattttag aattttaata caaatagtgt tgcctttaat taaaccagca ttaattacac
    13861 tagcaatatt tacttttatg ggagcatgga acgagtttat gtggcctctt atagctacaa
    13921 ctaaagatgc aatgagaaca ttaacagttg gattatctac tcttaagaca ttccagataa
    13981 ctaattatgg acagatgatg gcaggggcta caataacatt tttacctcca tttattttct
    14041 atttgatttt acagagtaaa tttgttgaag gtgtgtcttt gtctggtata aaaggttaaa
    14101 tagtctaatt taataatata taaagaaact tatataaata tggaggaatt taaactatgg
    14161 aaatttcata tgtactgaaa gattgcacat tcaaacacaa taaatactca gttgtagaag
    14221 ctgaaacttg gaaagataag gatttggata ttgtagtagc taaaggtgaa aaatttgcat
    14281 ttcaaattat gttaaaagca acagaagaat ttaattgtac aattgataaa agtagtgcta
    14341 tatcatggaa aggcctggga aatagagtaa ggttagcatt aaatatgcct aatagtttag
    14401 aaaataattt tagtataaat atattaggat atgttcaaga tgatacaaag gcatttgtga
    14461 atgatgctat tttaagggat aaagatgtgt tagtagagca atatttgcct caaacatttt
    14521 ggattgaagg tcaagtacca gaagattttg aagaaaataa ccttgatata ggtattgata
    14581 tatttaaaag ctttggatat gaagatgaag aaaaagtttg tacaattgat gttcctgtaa
    14641 aagtaagaaa tgttgtgcta aagcctttag atgaaagcaa gttcttttta gatttatggc
    14701 aacatccaag ctgtttagct agaatgtata aagttgagtt atggtctgat atacattttg
    14761 aaatagtcga taactattta aaagaattag catctttagg agaaaaagtt gcaacagtta
    14821 tagtttcaga ttatccatgg gcagggcaaa gttgttataa agtttataaa aatccatcaa
    14881 acttatatga atataacatg gtaagtgtat caaaaggatt ggatggaaag attaaatgta
    14941 actttgagag tatggatagg tatataggca tagctgataa gtataaaatg gctaaggaga
    15001 tagatttatt tggtctatta ggtaattggt gtgctggtga gtttggaaat cctgtagaag
    15061 gttataagga ccctataaga gtaagatatt ttgatgaaaa ggataaagta tttaaattta
    15121 taaacaatac aaatgattta aaagaatata taggattagt attaaatcat ttaatagagt
    15181 gtggattatg ggacagagta aggattatag ctgatgaacc aaataatcca gaagtagtca
    15241 aagagtgtat agaatttata aattctactg taggtactca tcaagttaaa tataaatcag
    15301 caactcacga ccaaaacttt ttagacagag ctaaagatga aattgatgat atgtcaataa
    15361 atttaaaact tactatacaa aattataaag atatagaaag tcttaagaaa aaaatcaatg
    15421 acaagggtgg gatattgact tggtttgtat gctgttttcc agaaaaacca aatagttttt
    15481 taagttctcc ttttgtagaa aatagaataa taggatggta tacttattac tttggtcttg
    15541 atggattttt aagatgggac tataatttat ggacagaaga cccttggaaa gattcaagtt
    15601 acaaattccc tatttggaaa gctggagata tgttctttgt ttatcctgga aaagatttaa
    15661 agccagttag aagtgtcaga atggagaatt taagatttgg aatacaggat tttgagttat
    15721 ttacaatgtt agaaaaagaa aaggtaagag agtatataga agttgagctt atgcaagaat
    15781 tattaaataa aaaagaaaat gctgaaatta aaggttttgg tgacatagaa ctaggatatt
    15841 cacttgataa ttggagatat gataaagtta aaaaatatgt attagagttg ttagacagag
    15901 tataaaaaat ataagagtgt ctcaaaatga actttttagt tcatgaggca ctctttttgt
    15961 atgaaatcaa tatagtttca ttgtttcaac aatgaaaaat aggattattt ctattctaag
    16021 acagcccttt agttttttag ttgtattcaa tgttttaaaa ctaactataa acgctataaa
    16081 gtttataaat gacaataatt atatataaca tgatgtactt taatttgcta taatagggat
    16141 tgaataatat tatatggaag ataacattgt ttggggggat taatataaaa agaataggta
    16201 ttattataat gtttttttta agtatatctt ttattggatt ttatcttgta ataagagata
    16261 acagtgatac agttaagatg caacgaggga taaatatagg taatgcacta gaatcaccta
    16321 aggattttcc gtgggatgta aaaatgtcaa ataagttttt tgatgatata aaagatgcag
    16381 gttttgacac agtacgtata ccagtaaggt tttctgatta tacgtcagat tctgataatt
    16441 ttaaaattga tgaagaattt tttaaaaaga ttgataaata tgtagattat gcattggata
    16501 aagacttaat agttgtgttg gatttacacc attttgaaga aattatgaaa gaaccaagag
    16561 ttcataaaga aaagttttta aaaatatggc aacagatagc taataggtat caaaaatatg
    16621 ataaaaaatt ggtatttgaa ttattaaatg aacctaaaga aaatttatct tcacaattac
    16681 taaacgaata cattgaagaa gcaataaaga taattagaaa aactaacccc aaaagaacta
    16741 ttatagtagg accatataat ttctatcaaa ttgattattt gaatgaatta aatattccta
    16801 aagattctaa tatagtagtt tcttttcact attacgagcc aaatgatttt gcatttcaag
    16861 gaaatatcta tcataaggga tttgagcact taagcaatat tacttgggaa ggaacaaatg
    16921 aacaaatgga ttatttaaaa aaaaggtttg atactgtgga aaattgggca aataaaaata
    16981 aggtaaaaat cttcttgggt gaatttggaa taacaaaaga ggcaccagaa gtttcaagaa
    17041 gagcatggat aaaagctgta agagaagaag ctgaaaaacg aaacttttca tgggcttatt
    17101 gggagcttgc atctggattt ggtatataca atcaaattga agggacttgg gatagagata
    17161 tattaagtgc attaatagaa aagaggtgat tttatgaaga aaaattcaaa aaagatactt
    17221 ataactttaa ctattattac taatgtcata tatatattgt ggagaatttt ttacacagta
    17281 cctaaagaag aagggaagtt tgccttaata tgtgccataa tacttctttt tgtagagata
    17341 atgggtatga tggaaatgtt tgtgcattat tatggtatgt caaatattga gtatcctgaa
    17401 aaaccaatca taagtgaaga actttatcca catgtagatg tatttattgc aacatataat
    17461 gaatctgttg atttggttag aaaaactgta aatggatgta ttcatatgca gtatccagat
    17521 aagaaaaagg tacatatata catatgtgat gatggaaatc gtgaagaaat gcgtattcta
    17581 gcagaaaaaa tgggtgtaaa ttacataact agaactgaaa gagaaggagc aaaagctgga
    17641 aatttaaata atgcaatgca acatacaact tctcctttga ttgctacatt tgatgcagat
    17701 atgataccaa tgcatgactt tttaattgct acagtaccat actttttaaa aaatgagcaa
    17761 gctaaaaaag atggagaaaa agaggagtat gaaaaggttg gttttgtgca aactccacag
    17821 agtttttata atccagattt gttccaattt aatctgcact ctgaaggaag gattccaaat
    17881 gagcaagatt atttttacag agatattcaa ttggctagaa ataggacaaa ttctgtaatt
    17941 tatggtggta gtaatacagt tatttcaagg gaagcacttg aagaagttga tggattttat
    18001 acttattcta ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa ggggtataga
    18061 tgttatgcta ttccagaagt acatgcatca ggtttatctc ctacagattt aaaaagtctt
    18121 attaagcaac gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg tctaaatatt
    18181 ttgtttagga gaggtttagg tttttggcag aaaataagtt atatttcttc aattacttat
    18241 tggtatgcaa gtataaaacg ctttgtatat ataatggcac ctattttatt ttctgtgttt
    18301 aatgtgattg tagtaaaatg tacgttactt caagtattgg tattttggtt gccaatgtat
    18361 attttaagta gtttatctct taaaatattt tctcaaaata taagaaatac tagatggacc
    18421 aatatatatg aaactataat gtttcagtct ttaatgccag cagtaatact tgaaacgttt
    18481 gcaatatcta aaaataaatt ttcagtaaca aataagagta aattagaaga aaatagaatg
    18541 tataagtttt tacagggaat accttatttt atttacatgg tcttatctat cataggaatt
    18601 ttaaaaatgt ttgtagcaat atttaaaatg agttcaatga catattcagt tgtattattt
    18661 tggttgattg gaaacctttt taaccttgtt atggctacat tatttatatc agggagacag
    18721 cagttaagaa agtctgagcg ttatattgct gaaattgatt ttaagttaaa gcaaaattct
    18781 tatgttcttt cttcaaaaac aattgatata tcagaaaatg ggtttgcatt tttgctagaa
    18841 aatccagaat atatttcacc agaagaagag tttgaggtag agtttagaga aaaatctgga
    18901 aatgaaatgt atatagccaa tatgaaagca aagattgtta atgttgtgga agtaaattca
    18961 aagtggaaat atgcagctta tattacacat attgaagata gtgaaataga taattggatg
    19021 tgtattgttc acgatagaat acctacttta ccaatgacaa tatctaatca actaggtttc
    19081 tttgatgatt tgcaaattaa tgtgaaaaaa cgtattgaaa aaactagaac attatcacga
    19141 agaagtccaa gaatcaatat gaattttcag atggatataa aaaatacagg taaattaaga
    19201 atagtgaatt ttaattatca atatgtatta ttaaattttg agaataaaag tatttatcct
    19261 aaagaaatag cattggaaat taatgaaaat attgtgttag aatgtgattt gtgtgagggt
    19321 aaaattgatg aaagaggtat cttatataaa gtaaataaca ttgacagcat aatgcaaaat
    19381 ttctttttaa gagatgaaat gatggattgg atattacaaa ataagacaat tcttgtttca
    19441 aagcctagtg aaaagaagga aaaaagtata gatgagtttg aacccatgga atatatttag
    19501 gagagatgaa atgaagactt taaaagtgtt tttttgttct acaaaatatc ttattttggt
    19561 gtttattttt tttataaata ctacttttat aagttcagca acaagtgata aaattttaga
    19621 tttgtccttt aaaaagatag agactgactt aaattctaag attacatatg aagatacagg
    19681 tgtaaaaata gaaacggata gttctaaaag cgataaggaa cgttatttat atatttacca
    19741 aaatataaaa gaaaattgga gcatgtataa taatttttat attgaaattc aaaataaaaa
    19801 taaaagttct cagagaataa atttaagcat acaaagtaaa aatatgcttg aatttagatt
    19861 aaaagaaggt agtgaagtat ttttagaagg taaaaatata atatattcag ataaaataaa
    19921 agaaggatgc atagaagtac caggtgaatt tgaaggcaaa atttatgtga actttaatag
    19981 ccttataaat gaggaaagta atgttgtgct ggactcaaat atgctttcaa acattgttag
    20041 ttggggaatt acgtttattc catctgatga agaacataat attgttatta taaaaaagat
    20101 atctttattg tctgaagaga aattaagatt tttaaataat attaaaataa ttggcgatga
    20161 agaagtacaa ataccagtat tgggtcaaag tataagtcag tatgaagtat taggtcttaa
    20221 aagtgattct aaaattaagt actctcttat gggaaaacaa gataatgtaa gcatatctca
    20281 aaaaggtaaa ttaacattaa gtaataaagc caaacctgga caaattattt tacaagttaa
    20341 tgttgatgat aagtttaaaa ttggaaagaa aataacatta actgagtctt ggagtgttaa
    20401 taaaaaagat aaggatggtg ttccatatac attagtatat cctgagcaat caccaacagt
    20461 acaagatatg aaaaaaataa actttatgaa taacatcata acgtttgttc gaattttatt
    20521 tgtatctcta gtaataatat gctttggaat atatttatat tggaaaaaat gttcaaaaac
    20581 taagtaaaga gaggaggtta gattgtgtta ttttcaagtt taattttttt attttatttt
    20641 ttacctataa ctttggtttt atattatgta tttaggttta accgaacgat acagaacatg
    20701 atattgcttg ctgttagttt atttttttat gcttggggag agccaaaatt tgttgttatc
    20761 atgatagttt ccataataat gaactatata tttggtttac tggtagatag atatagggaa
    20821 agtaaaataa aagtaaaagt ttttttagtt ttgatgtgtg catacaatat aggtgtttta
    20881 tttatattta aatatctagc ttttgcgcta agaaatatta gtgaacttat tagtactgaa
    20941 ttgactatac ctagtattgt tttacccata ggaatttcat tttttacatt tcagggaatg
    21001 tcttatgtta tagatgtata tcgtagacat ggagaagtcc aaaagaaccc tttttatgtg
    21061 ggattgtata tagcattttt cccacaatta atagcaggtc ctatagttag atatgagagt
    21121 gtggcagaac aaattctcaa tagaaaagaa acttggaata aattttctat tgggacttgt
    21181 aggtttataa caggtctagg taaaaaagta ttaatttcaa ataacatggc tatagtagca
    21241 gactatattt acactatgaa tagtcaagga gaaatagctg catcacttgc atggcttggt
    21301 tcaatagcct atactctgca aatattcttt gatttttctg cttattcaga tatggctata
    21361 ggcttaggtc ttatgtttgg ttttaaattt gaagagaact ttaattatcc atatatatca
    21421 aaatcaatta gtgagttttg gagaagatgg cacatttctt tgggaatgtg gtttaagagc
    21481 tatatatact ttccgcttgg aggttctaga gtagcaaata aagatattat gattagaaat
    21541 atgtttatag tgtggttatt tactggaata tggcatggag cagaatggac atttgtaatt
    21601 tgggggattt taaactttgt tttcctaatt atagagcgat ttataatgtt tgaaaagatt
    21661 gaaaatcaca attttataaa acatatttat gctttattag tagttaattt tgggtgggta
    21721 ttatttaggt cacctaattt aaaagaagca tataattatt ttaaagctat gtttggaggc
    21781 aatggagcta tatggagtga ttatacatat atgtttttaa aagaatactg ggttttcttt
    21841 atatttgcat ttatttttag tgtacctatt gcaaagaaaa tcaataagtt tgttgttgaa
    21901 caagctaaat atagtatgat atttaacgta ttttatccaa tctcagctat tttattgttt
    21961 tttatttctg tgacatattt agtaactgga agctataatc catttattta ttttaatttt
    22021 tagttgggag gaaaagtatg aatagatttt ttaaagtaag attaataact gcaattatgt
    22081 tcttaattat tttattcgct ttctctatct ttaatgtttg ttattctgga aaagatacga
    22141 tacaggaaat aaaaaattca atagctgaaa aaagtagttt aaaagataca atatcatcta
    22201 ttgataatac agtgaatgac aaagtaatat acaaaaatac gttcattgaa acttatggct
    22261 atttacaaaa attaatgggt aaaaatgagt tttcaaaatt tagtgttgta aaagatactt
    22321 ctggaaagat gcattataca tattttgcta aagctccaaa tccagttggt gtgatagcag
    22381 atagggtaag aaaattttct gatgaaatgg aaaagcaaaa cactaaattg gtttacctaa
    22441 tgactccaga taagtatata aaaggagtga caaaatttcc aaaaggaata ccttattctt
    22501 atagtaatga gacagcagat aattttttaa atcaactaaa aaagtataat gtggactata
    22561 ttgattttag agaaaatatt ttaaagagtg gtattccaaa agatgattta ttttttaaaa
    22621 cagaccatca ctggaaagta gaaacttctt tttgggcatt tggagaatta gttgagcaac
    22681 ttaataaaaa atacaatatg aatttagatg aaaatcatta ttatagagat aaggacaatt
    22741 ataattcaat tgtatatcct aagtcatttc ttggttctat gggaagaaag actggtatct
    22801 tatatggtgg tatagatgat tttacattaa tttatcctaa atttaagact aattatactt
    22861 attatacaga ttctaaatct caaaaatttg aattaaaagg aaggtttgaa gagagtttaa
    22921 tattatcata tccttttaat gcagaccttg atttaatgga tggtcaatca gataagtatt
    22981 ttacatattt attggggaac agaccattag ttaaaataaa aaatatagaa aatccagatg
    23041 gattaaaagt gctgtttgtt aaagattctc taattgtacc aacagcagca tttttttcct
    23101 ctgtttgttc atctatagat atgatagacc ctagatatta tgatggagat atactagaat
    23161 atgcaaaatc ccatgactat gactttgttt ttatatctgt atatccccaa aatcttacaa
    23221 aagaattttt tccgttttgt gagtaaattt ctaaaaaagt tttctgataa aataaatggc
    23281 taactggtaa aaagttagcc atttattttg agtgttattc taagaaatat tttttttact
    23341 aaatacttct ttatctattt ttccaagtga ttttcctaca gtaattgatg ataccatagc
    23401 accattaaca tttatcatag tacgtcccat atctagtata gggtctattg caagtatacc
    23461 accaactaat ggaaaataag aaccaagacc aactccagag ataacaactg aaacggccat
    23521 tgtagcagtt cctggaagac cagctattcc aaacgaacta attgttataa caattaacaa
    23581 cataacataa aagctcatat ccatgtttac tccagccata ttggcaagtg ttacagccat
    23641 taaagcagga tatatacctg cacaaccatt catacccata tttgcaccta aacttgctgt
    23701 aaagcttgat acaccctcat caactccgtg attttgtact agactttcta tcgttacagg
    23761 caatgttcct aaactagaac gtgaagtaaa tgccagaagt aaagcttcag ttgaattttt
    23821 tatataagta atgggattta gaccatttaa agttataaaa attaaatgta taataaataa
    23881 tattgcaact gatatataaa gagctaatat aaactgtaaa acagatacta ttgaactaat
    23941 accttgtgaa gttattgtat ttgcaagcag agcaaccaca gcataaggca taaatttaat
    24001 aacagtcata gacacactta caattatttt gtaagatgct tctataaaac ctataaatgg
    24061 ttttattgtt tcaaaatgtt ttttacttaa tctctttatt gaggtagcta taaatgtagc
    24121 aaatattatt gtagctacaa tatttccatt tgccatagcc attacaggat tagatggtag
    24181 taatcctctt aaagtatcca caagagagtt tatttcttta agttcttgat tttcaactaa
    24241 agtaggcata ttagctccta cacctaactt cattatattt gcaacgataa ttccaacaat
    24301 tgcagctata gcagtagtag caagaagcat acctaaagat ttaaatgtca gagttccaag
    24361 attgttatct ttcatattta taattacttt tataatggat aagaaaacta aaggtactac
    24421 taacattttt aataagtcca taaatccaga accaaatagg ctataccact tacttacttc
    24481 ttgtagccaa atgacttgag ttgtatcatt aggaaaacca gcaaaagctt gtattatgat
    24541 tcccaatatg agtcctagta ctgtagcata tattgttctc aatgaaaatt ttattttttt
    24601 cttttcaaat tgtctaacaa taaagaacgt acctagaagt aatattatga aaaaaattgt
    24661 tttaaaatct gtaatcataa gaaattgtga taaaaatgaa ctttccaata tttttctcct
    24721 tttatttaaa aataaatttt tctaagttta aatgatatca atatattaaa aaaatgtcaa
    24781 tattatatca caatttactt aaatagagta aaacaagttg attttgaaaa atgtgattaa
    24841 gtacagaaaa atagggtata ataaatgttt aaagaatttg aattgtggta agaaaataaa
    24901 aaatagcttt tataagattt gatttcaaat taaaatgtgt taaataaata actaaaaatt
    24961 attataaaaa atgttttcat agaatatata tcaaatgaaa agcttaaaat aagtgaagaa
    25021 attaactaga catcaaagga gagataaaat gagagtaata ataattggtg gtgttgcagc
    25081 tggtatgagt gctgcagcaa aattaaaaag aataaaacct gagtatgaag ttgttgttta
    25141 tgaaaaaact gaaatagtat cttttggtgc ttgtggatta ccatactttg ttggtggatt
    25201 ttttaatgat gctgatgaat tattagcaag aacaccagag aaacttagag aagctggaat
    25261 tgatttaaat atatttagag aagtcgttga ggttgatagt gaatctaaga aaataaaagt
    25321 aaaaaacata aaaacagatg aaatatatga agattactat gataaattga tgatagcaac
    25381 tggagcaaga agtattatgc ctcctataaa aaacataaaa ttaaagaatg tttctacatt
    25441 aaaaagttta tatgatggag aatatttaaa aaaactttta agtaatgaag ataataaaag
    25501 agtgacaata ataggagctg gttttatagg gttagaagca gttgaggcat gtaagaaatt
    25561 aggtaaagat gtagatgtaa ttcagttaga agatagaata ttgccacaag tatttgataa
    25621 ggaaatgacg gatgtattag aagaagaaat aaatagacat aatgttaatt tgcacttaga
    25681 tgaaatggtt gtagaacttt gtggagaaga taaagtagaa aaagttataa caaacaaagg
    25741 ggaaatagat acagatgtag taataattgc tactggcgtg agacctaata ccgagttttt
    25801 gtcaaatact aatataaaaa tgctaaaaaa tggagctata gtagttgatg agtatggaag
    25861 aacttctgta gaagatatat actctgctgg agattgtgca actataaaga atatagtaag
    25921 taatgaaaat gtatatgttc ctttagctac tggagctaat aaattgggta ggatagtagg
    25981 agaaaaccta gcaggaagag aaatatctta tcaaggttca ctatcttcaa gctgtataaa
    26041 aattatggat atggaggcag catcaactgg aattacagaa aggcaagcta aagatttagg
    26101 cataaatgtc aaatctaaat ttatttctga ttataatcaa actcattatt atccaggaag
    26161 aaataaaata tatgtaaagc ttatttatga tgcagataca aaagttattt taggcggaca
    26221 agtggctggg tttaaggatg ctgtccaaag agcaaatgta ttggcagctg ctatctttgg
    26281 aaaaatgacc acaaatcaac ttggaatgtt agatttatgt tatgctccac catttgcaag
    26341 aacatgggat gttttaaatg tagctggaaa tgtatctaaa taatatttag attatagcta
    26401 atatagttga caagcaatta agggaatgaa tttacctaaa ctatttttat catggtaaaa
    26461 ttcattccta aatttttatt ccaaattttt attccaaatt tttagaaata aggattccat
    26521 taaattattc atcaaaattt aaacgcaagc ttaatctatc ttctccaaga atagtatttt
    26581 caaaaacatt agtagcattt tctaaataca tactaggtat gtctaaagaa ggactaaaat
    26641 gagtcagtaa aagttgtttg acatttccaa gttttgcaag attagcagct tctctaaaag
    26701 tcatatgttt gtttctaaca gcttttgata tatctaaatc atcaccatac atggcttcac
    26761 atataaataa atcactatct tttatgaaat ctggtattga ttctataggt cttgtatcgg
    26821 ttgtaagact tatcttaact ccctttctca attctcctaa gaccatatct ggagtgtatt
    26881 gttttgaatc taaaactata ttttgacctt cttggagttt gttccataat attttaggaa
    26941 cttcgttttt agttgcctta tctatatcaa atttagggtt tcttctaaaa ttaaaactgt
    27001 atcctaaaca ctctatagag tgttctaaac tgattgttga aatttctaaa tttcttaaaa
    27061 ctttattatc taaagaaaaa ttgccttgag ggttttctat aatttttaaa gtgtatggta
    27121 catattctac taaatttctc atagaacgta tacaatcgac tattccaact ggaccaacta
    27181 tagttaaatc acttgtacga ccactattcc caattgttga taaaagtcct aaaagtccaa
    27241 atatatggtc accatgtaaa tgggttatac aaatcaagtc tatatcttta aagccacatt
    27301 tttttagctt catagatacc tgagtacctt caccacagtc aattagtatt tttctacctt
    27361 tatagtttat aaatacagag gatagaaatc tgtttggcat aggtacattt ccaccacatc
    27421 caagtaaagt taaatctatc attttttccc ctttctaaaa ttaacacaaa tctgttgttt
    27481 gtttttatta tgatatccta ttataattat tttaacatta aatataacaa taaatgtgta
    27541 tttgagtcaa gaaatattgt caaataaagt ttttatatag caatgtataa aaatttggaa
    27601 aacgaaaagt gaaaattata tttaggagta tttaaaggag gaaaatttaa tgaaagtatt
    27661 aaagaaaatt actattttgt cattaattac attaacctta gttggttgta gtaacggaga
    27721 agagaaaaaa gtggttgaag gttctaagaa aactcaaaat tctaaaaaag atgaacagcc
    27781 tataatagta cataaagatg caaatatatt gaaagatagg tatgagccac caaaaggatt
    27841 taaaagagtt gatgtagaaa aaggtagttt tgaatcattt cttaggaata ctaagctaaa
    27901 aaattatgga gagaaagtaa agtattatga tggaagaact aaggaatcaa aaggtattta
    27961 tgatagtgta tttgatgttg atattggaaa tagagattta catcaatgtg cagatgcaat
    28021 tatgcttatg agagcagaat acttgtatca gaataaacta tatgatgaaa taagttttga
    28081 ttttgttgat ggttttaagg cgaagtattc aaaatgggca caaggatata gaatttcagt
    28141 aaaagatagt ggttcaagct ggtataaagc aactgaatat tcaacatctt atgagagttt
    28201 cagaaagttt atggatatag tgtttgcata ttcaggaacg ttatctcttg aaaaagaatt
    28261 ggagcctgta aaagttgagg atatgaaaat aggagatgtg tttatagttg gtggaagtcc
    28321 aggacatgca agtataatta tggatatggc agaaaatgaa aagactggtg aaaaggtatt
    28381 tatgatagcc caaagctata tgcctgcaca acaaacgcaa cttcttatta atcgtaaaaa
    28441 tccaaatttg agtccttggt atagtttaga ttttgaaggt gatttgacaa tacctcaatg
    28501 gacttttaaa agagaccagt tgaaaagatt ttaaaataat aattttatat aataaaagaa
    28561 aaatatagat gtgaaaaaat gtaattagat taaaactaat tggatatgga caaaattaaa
    28621 ttgatagata aatattaaaa attacactaa ttaatcttac tatagtttaa tatttatcta
    28681 aaaagtttta tgtaaaatat aaattttata ttaaagctaa aactaatttg aggaggctaa
//
LOCUS       DAYASV010000038        28473 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYASV010000038 DAYASV010000000
VERSION     DAYASV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.038"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            549..1856
                     /locus_tag="ACGI68_001651"
     CDS             549..1856
                     /locus_tag="ACGI68_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138093.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEAFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1942..2204
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2330..3133
                     /locus_tag="ACGI68_001652"
     CDS             2330..3133
                     /locus_tag="ACGI68_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0138094.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3171..3782
                     /locus_tag="ACGI68_001653"
     CDS             3171..3782
                     /locus_tag="ACGI68_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFH0138095.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3814..4485
                     /locus_tag="ACGI68_001654"
     CDS             3814..4485
                     /locus_tag="ACGI68_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFH0138096.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4784..5146
                     /locus_tag="ACGI68_001655"
     CDS             4784..5146
                     /locus_tag="ACGI68_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFH0138097.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            5582..6283
                     /locus_tag="ACGI68_001656"
     CDS             5582..6283
                     /locus_tag="ACGI68_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HFH0138098.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDELCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            6554..7372
                     /gene="larE"
                     /locus_tag="ACGI68_001657"
     CDS             6554..7372
                     /gene="larE"
                     /locus_tag="ACGI68_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HFH0138099.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            7427..8575
                     /locus_tag="ACGI68_001658"
     CDS             7427..8575
                     /locus_tag="ACGI68_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFH0138100.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            8711..10099
                     /locus_tag="ACGI68_001659"
     CDS             8711..10099
                     /locus_tag="ACGI68_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0138101.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            10128..10826
                     /gene="walR"
                     /locus_tag="ACGI68_001660"
     CDS             10128..10826
                     /gene="walR"
                     /locus_tag="ACGI68_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism DNA-binding response
                     regulator WalR"
                     /protein_id="HFH0138102.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            10829..12262
                     /gene="walK"
                     /locus_tag="ACGI68_001661"
     CDS             10829..12262
                     /gene="walK"
                     /locus_tag="ACGI68_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFH0138103.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            12284..13021
                     /gene="truA"
                     /locus_tag="ACGI68_001662"
     CDS             12284..13021
                     /gene="truA"
                     /locus_tag="ACGI68_001662"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFH0138104.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHSPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    13132..13339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13592..14791
                     /locus_tag="ACGI68_001663"
     CDS             13592..14791
                     /locus_tag="ACGI68_001663"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFH0138105.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            15038..16084
                     /locus_tag="ACGI68_001664"
     CDS             15038..16084
                     /locus_tag="ACGI68_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HFH0138106.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNDSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTGQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            16192..17355
                     /locus_tag="ACGI68_001665"
     CDS             16192..17355
                     /locus_tag="ACGI68_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0138107.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFDVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     NRNPSSTDLQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(17444..17824)
                     /locus_tag="ACGI68_001666"
     CDS             complement(17444..17824)
                     /locus_tag="ACGI68_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HFH0138108.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(17827..18486)
                     /locus_tag="ACGI68_001667"
     CDS             complement(17827..18486)
                     /locus_tag="ACGI68_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFH0138109.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(18592..19566)
                     /locus_tag="ACGI68_001668"
     CDS             complement(18592..19566)
                     /locus_tag="ACGI68_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0138110.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(19606..19953)
                     /locus_tag="ACGI68_001669"
     CDS             complement(19606..19953)
                     /locus_tag="ACGI68_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138111.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(20171..21127)
                     /locus_tag="ACGI68_001670"
     CDS             complement(20171..21127)
                     /locus_tag="ACGI68_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFH0138112.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            21572..21940
                     /locus_tag="ACGI68_001671"
     CDS             21572..21940
                     /locus_tag="ACGI68_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
                     /protein_id="HFH0138113.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            22365..22610
                     /locus_tag="ACGI68_001672"
     CDS             22365..22610
                     /locus_tag="ACGI68_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138114.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVVNKVKTG"
     gene            22709..22972
                     /locus_tag="ACGI68_001673"
     CDS             22709..22972
                     /locus_tag="ACGI68_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HFH0138115.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(23257..24204)
                     /locus_tag="ACGI68_001674"
     CDS             complement(23257..24204)
                     /locus_tag="ACGI68_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0138116.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLANTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(24207..25838)
                     /locus_tag="ACGI68_001675"
     CDS             complement(24207..25838)
                     /locus_tag="ACGI68_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0138117.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            25968..26885
                     /locus_tag="ACGI68_001676"
     CDS             25968..26885
                     /locus_tag="ACGI68_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0138118.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            complement(26942..27523)
                     /locus_tag="ACGI68_001677"
     CDS             complement(26942..27523)
                     /locus_tag="ACGI68_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0138119.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(27563..28129)
                     /locus_tag="ACGI68_001678"
     CDS             complement(27563..28129)
                     /locus_tag="ACGI68_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0138120.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQINNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
BASE COUNT        10980 a         3232 c         4497 g         9764 t
ORIGIN      
        1 tgaaaaattt atataagtaa aatatattaa cataaaggta aaattttgta tactaaaagg
       61 gaatatttac agtttaatgg cagttttaaa ataataaatt aaagctgtca tttttattta
      121 aaaattttta gaaaaagtac aatagtgtta atgtgtgaat tagataaggt gtatataata
      181 gtttagatga tatgatataa aatatgttgt aaattaaatg ttttctttga caatatatac
      241 acaaggatga taatatctat atgaataaaa gatagtgtgt aatttgataa tatgtaatta
      301 taacattatg atataattac aataaagtat gtaataatct gtagaaattt caagctagtt
      361 atctaagttt tcggataact aaatctaaac ggaatacata atgtattaaa taaatgatga
      421 tactaaaatg atactatttt tatagaaatt tattgaggta tataattaat gaaaataata
      481 tattaaatta tataaattaa attatataat atattgagtt ttacataaaa gcaaaggtgg
      541 gaataaaaat gaaagaaatt gctgatgcta taacatcaga gtatttaagc tatggtatca
      601 atgaagatat tagtatctat gaaggtagat tttgcatata tttagataaa aaatatagat
      661 gtaatgggaa aatttattat aaaatgacac ctccaatatc tataagtttt aaagcagaca
      721 taggttgtgt tgaagagata gacaatgaag atgacaattt agctttagat tatgataatg
      781 ctattcttga agttcatgga tataaaatta tttctataac aataaatact ttaagtgaat
      841 ttagtgtaga aggttatatc aatgatgact gtataaaatc taagaactcc tatgttgaat
      901 atgtagattt taatataata aacttagata aaatacctgg aaaactcatt aaatacaatg
      961 ataaggtata cgcaggtaga attgaatttg atataaatga ttatgttgta actatagaca
     1021 agcgatatga ttatagaaaa gaattaaaat cagaattaaa gtctaaatct ggagcaataa
     1081 taactcatat aggcaggatt agaagaaaag atggtagaat ttttagaaca aataatacaa
     1141 taaatctttt agataggata tcaacagctt tgagttttat gtgtggaaga tatgtagggt
     1201 tttgtttggc caaagggtat agaagtggta atgaagttta tagaatatgg aatgaaaatc
     1261 aaatatctcc ttttagatat gtaccaacat ggtcagatac actttcaaat tatcataata
     1321 tggaaaaata tataagcctt atgtgtaaaa agctagaaga tttttattat ggttctgcta
     1381 taaagagtgt tgttgattgg tacatagaat ctcttggaag tgctaccatg gaaaacaata
     1441 taatatctgt acaaattgca cttgaaactt tgtcatatgt aattctagtg gaacaaaata
     1501 agatacttac agatgaagca tttgactgta atttggcatc taaaaatata agactcttac
     1561 ttgatacatg taagatacca tatggtaaac atgaattaaa tatatttgat aatataataa
     1621 aaaataaatt tgatgatgga gtggatttag ttatttattt aagaaatagc atagttcatc
     1681 caagtagaaa gactcataga gctgtgttag aggttgaaga tatttggaat ataatttcaa
     1741 taggaacgag atatattgaa cttgttttac tctttatttt aggttataga ggagaatatt
     1801 caaacagatt agtagaaaga tgttatggtg aagtggaagt tgtaccttgg aactagattt
     1861 ttatatttat tttaaaatat atattgacat atattgcctt atgctataaa atcaatatca
     1921 taataataat tcagaataac attaaatgct aagaaaagaa atagtaaaag tagttaatct
     1981 taaacagaga gtttccgatt ggtgagagga gcaatagatt ttactttgaa cacatctttg
     2041 agcaggactc tgaaagagta gaaatacttt attaggagat ttccggaatc cttgcacccg
     2101 ttatagtgct agagtataat aattttattg tacttaaaga ggttagtgcc gtgaggcatt
     2161 aatgaacaga ggtggaacca cgagagttaa ctcccgtcct ctagcagaag ctagaggcgg
     2221 gagtttttta atataatttt ttaagcgcgc agagaattat attgcaatag atacactttt
     2281 taaaaaataa tcaaaatatt ttaaaaatta aaagacgagg gagaatatta tgaaaaatat
     2341 attaaaaaaa gtaggaatat ttacaattat gttaggttta ttaggtggag ttgtaggttg
     2401 ttcaaaacca gataatgaaa aagataagga tgcttcaaag gaatctaaaa aagaagtagt
     2461 tgtagggttt gataatacgt ttgtaccaat gggattttta gatgaaaagg gagatacagt
     2521 aggctttgac gtagacttag ctaaagaaac atttaaaaga cttggaatgg aagttaagtt
     2581 tcagcctata gattggtcaa tgaaggaaac tgaattaaat gattcaaaaa ctgtagatgt
     2641 attatggaat ggatattcta taacagacga aagaaagaaa atagtatcat atacagaacc
     2701 atatttacaa aataaacaga taatagttac tctaagtgac tcaaaaataa attcaaaagc
     2761 tgatttaaaa gataaagaag ttggaacaca acaaggttca acagcacttg atgcagtgga
     2821 aaaggataaa gattttatga atagcttaaa aggtggagca ccagttcttt atgatactta
     2881 tgataaagca cttagagatt tagagatagg tagaactagt gcagttgttg gtgatgaagt
     2941 tttaattaga tactatatgg gacaaaaggg tgaagacaaa tacaaggtat taaaagacga
     3001 ttttggatta gaagattatg ttgtagcaac ttctaaagaa aatccagaac tttgtgagaa
     3061 aataaatgaa acattaaaag aaatgaaaaa agatggaact tttgacaaga tttatgataa
     3121 gtggtttaaa tagaagataa gttagctgtt aagaaggggg cactaaaggc atgttacaag
     3181 gtttagagat agttatagca atgttttgta taacattgat agtatcaatt ccattgggga
     3241 ttggagttgc atttttaaga ttatcaaaga ataaattagt aagtggtatt acacaatgtt
     3301 atatattgat aatgagaggt acacctttac ttttgcaaat gatagtcata ttttacggat
     3361 tacctctttt aggaattgta tttgacagat ttacagctgg agtagtagct ttcttcttaa
     3421 attatgcagc atattttgca gaaatattta gaggtggaat acagtcaatc gatagaggac
     3481 aatatgaagc ttcaaaagta ctaggatttg ataagtttac tatgtataag agagtaatct
     3541 ttccacaagt ttttaaaaga atactagcac ctatttcaaa tgaagtaatc acattagtaa
     3601 aagatacatc tttagtatat atattaggat taaatgatat tttaagaata tctcaaatag
     3661 caatgaacag ggaagcaagt ctgttaccat tatttgaggc aggagcgata tatcttatat
     3721 ttgtagctat acttactaag ggatttgaat tacttgaaaa aaaatattct tattatagat
     3781 aaagttacaa tattatagga ggaggttttt tatatgttga aaatcaaaaa cttaaataaa
     3841 tcatttaaaa aaaatagagt acttaaagat atttcttttg agttagaaga aggtcagatt
     3901 ggagttttac ttggtaagtc tggtgcagga aagaccacaa tattaaggtg tataaatggt
     3961 cttgaagaat ttgatagtgg agagattata atagataatg aggtaataaa aaataagaga
     4021 gatatggcaa aaatacgagg aaaaataggt atggtttttc aaaatttcaa cttatttcct
     4081 catatgacag tccttgaaaa tataattgaa tccccagtta atgtttttaa agttcctaga
     4141 aaagaggctg aagagagggc tagagaactt ttaagattag ttgatttaga agataaatta
     4201 aattcttatc catttgaatt gtcaggagga cagcaacaga gagttgctat agctaggtca
     4261 tgtgcattga tgccaaaagt cctttgtttt gatgaaccta cttctgcact tgatattgat
     4321 actattcaaa gagtagtcaa tataatgaac agacttaaag acaaaggtat gacaatatta
     4381 ataattacac atgatgttgt cttctcaaat aatgttgcag ataaaattat aagtataaaa
     4441 gatggtatag tagaaaatgt tcaaataaaa gaaaaaattg tttagagttt tataaatata
     4501 aactagggta gaattttata atataaataa agggcaagta aaatcaaata tactatgccc
     4561 ttttgttatc tcaaaattta atcttatatt ttatgtttaa gttacattat aatttataat
     4621 aaaaataaaa ggactattta aatatataat gtgaataaat gtttaataat ttgaaatatg
     4681 ataatttttt atgttgaaag cttataatga ttatgatata aattggttat agtatacaat
     4741 ttatgggtat aacaataaaa aattagaaag gaatttttat aaaatgatta aattatatgg
     4801 atatacaaaa tgctctactg ttaaaaaagc taaaaattgg cttaaggaaa ataatttaga
     4861 atttgaagat atagatatgg ttcaaaaccc tcctagtaaa gaggagttaa aatcaattta
     4921 taagactagt ggatatgata taaagaaatt ttttaataca agtggtatga aatatagaga
     4981 attgggtctt aaagatattg taaaaactga atctgatgat aaacttttag aaatattggt
     5041 aagtgatggt atgcttatta aaagaccatt acttttggat ggtaaaaatg tattattggg
     5101 ctttaaagaa gatgtatgga aaagtacttt gctaaaagaa gattaggaag agataggtgt
     5161 gttgattaaa attattaatt aatacaccta taaatttatg tattaaatct aatttttata
     5221 aatattaatt agcaaattca aatcaaaaaa attcatatca acaaacatat tacttcttat
     5281 tacaaattat atcatatgtt ttatattaaa atattataat attacaacga attgttacaa
     5341 aattacttat tattaaccat gaacatttgt tttgtgctat catatttata taagggaatt
     5401 gtcataaaaa atatcttgtt catacagttt tatatagaaa aaagaaatga atcttttctt
     5461 tatataagta tattttgtaa attgcctcta tatttatagt ttaaataata gtggtattaa
     5521 aaatctgaca acaaaaataa aactttcaaa aaattaaatc taaaaagaat aggagataaa
     5581 tatgagtatt gaagatttat atgattttga atgtgttcct ttgaacaatg agattgatat
     5641 tgatgtttta tttgaaaaga tgatagaaga tggaaaagaa aaagaatata caccaattat
     5701 agtcattgat gaaaaagtag ggctattaag aaaaaatata gatatgatta ctaaagagta
     5761 tggttctctt gaaaactata gagagtattg tttaacaaaa tataatgaaa ttgatgtaaa
     5821 tcaatttttt gattttagga agagtgagat ttcagaatta ttaaaggagc taattgattt
     5881 agaagatgat ttttatgatt ataaacattt tagaccaatt atgaatatag atatatttca
     5941 tgaatataac aaagtttaca tagctaaaat tccaacaatt aaaccatatg aagtatttgc
     6001 atacatacct attggcggat ttaatgattg tcctagagat gaagaacata tagctattgc
     6061 aaaatattgg tatgaaaagc atggaatctt tccaatcgca attggatgtg atacagttca
     6121 atattctgta aaaaatttaa ctaaagatgg taaaaaattt gatgaattat gtattgaatt
     6181 agtattttat tgtgaggata ttattataca agggtatgaa accttaaagg ctcttaagga
     6241 tgtattaaaa cgctctacaa tagtgctttt ttggtgggat tgatattcca tcatttagat
     6301 agtattaaat tgttagtcac caagaatcca cttgctttag ttatggggag tgtgtcaaac
     6361 taggttttta tacacagtgt agcatagtaa tatgtttaaa ttacaaaggt taaaaaaata
     6421 ctttaatatt tttaatatta taattaatag atttaaaggt aattataatt tataaaataa
     6481 atactttata tgttataatc attaagaatg taaaaaaata atatatagtt ttatagttca
     6541 tatttttcca gatatggagg taaattttgt gaaggaaaaa gaaaaattag ataaattaaa
     6601 aaaaatgtta cttgaattag gcagtgttgt agttgcttat tctggtggag tagatagtaa
     6661 ttttttgctt aaggtcgcaa aagatacatt aggtgaaaat gttatagctg tgactattca
     6721 tgcaatgatg cattcaagta gggaaattga agaggctaaa caatatactc aaaattttgg
     6781 tgttaaacat atcatattaa atatagaaaa ctttgattta aaagaattta aagaaaatgg
     6841 tgttgataga tgttatcatt gtaaaaaata tatattctct aaaataaaag aggtagcaaa
     6901 agaacataat ataaagtaca tagtagatgg aacaaatata gatgatttag gagactatag
     6961 acctgggctt aaggcattga gtgaattagg agtaattagt ccattaaaag atagtagttt
     7021 aaaaaaagaa gaaattaggt cgctttcaaa aacattagga ttaaatactt ttaataaacc
     7081 atcatttgca tgtcttgcga gtaggatacc ttatggagtt gaaataacag atgaaaattt
     7141 aagaatagta gaaaagagtg aagaatatct atccaattta ggtttttctc aatttagagt
     7201 gagaatgcat ggagatatag ctagaataga agttggacag gaagaactag gtaaattttt
     7261 tgaaaataac aattttaata aagttgatac taaattaaaa atatttggct ttaaatatgt
     7321 tacactagat atgtcaggat ataaaatggg aagtatgaat ttaaatgtgt agattatttt
     7381 aaaaataaat aagttttata gttaatataa ggaaacagga ggttttatga aaaattatac
     7441 gttaatatcg ccatgttttt ttggaatgga aaaaatgctg gctagagaga ttactaattt
     7501 aggatatgaa ataataaaaa cagaagatgg aagaataaca tataaaactg atgagtttgg
     7561 aatagcaaag tcaaacatgt ggttgagatg tgctgaaaga gtacatctta aaattgctga
     7621 atttgaggca aaaagctttg atgaattatt tgaaaataca aaaagaataa actggtctag
     7681 atatatacct tatggagctc aatttcctat atcaaaagct tcctctataa aatcaaaatt
     7741 atacagtaca ccagatgtac aagccatagt aaaaaaggct atagtagaaa gcttaaagaa
     7801 aagttatttg gaggatggtt tgcttaaaga agataaggag aaatacccta tttttgtatt
     7861 tatacacaaa gataaagtaa ccatttctat agatacaaca ggagatgctt tgcataagag
     7921 aggatataga gaaaaggcaa ataaagctcc aataagagag actttagctg ctgggcttat
     7981 ttatttaact ccctggaaag caggtagagt tttagtagac cctatgtgtg gttctggtac
     8041 tatattgatt gaagctgcta tgattggtat aaatatggca cctggcttaa atagagagtt
     8101 tatatcagaa aagtggagaa ctcttgataa gaaaatatgg tgggatgtaa gaaaagatgc
     8161 ttttaataag atagataatg aatctaaatt taagatttat ggatatgata tagatgaaga
     8221 atctatagat atagctagag aaaatgctga aatagcaggt gtagatgaat atatagagtt
     8281 taatgtagga gatgcaactc aatttaaaag tgaagatgag tttggattta taatcactaa
     8341 tcctccttat ggagaaagat tagaagataa agacagtgtt aaacagttgt ataaggagct
     8401 tggttatgca tttagaaaat taaaaaattg gtcgtattat ttgataactt cttatgaaga
     8461 ctttgaatat gagtttgggc aaaaagcaga taaaaaaaga aaattgtata atggtatgtt
     8521 aaaaactaac tttttccaat atccgggtcc taagcctcct agaaataata aataagtttt
     8581 acatgattta tttgtaaaat ataaaatttt attaaaataa ataaaacatt atgatgcctt
     8641 gaaacgtcta attattgtga ggagattttt gtgaacaaat ttaaagattt ataggattag
     8701 gaggagttaa atgaaagtgt taaagcttat tactgtagta gccctgatga tgttatttgt
     8761 tactggatgt aatataaaaa ctgaatctcc agaacaatta gcgaagatac ctgactatga
     8821 tgatacaaaa aaagtattgt atgatggtat tgaccagtta ctaaaaccag attcatctat
     8881 gatattgcct tctaatacaa aagaagtagg aaaaataaat aaggtagatt taaacagcga
     8941 tggaattgat gaattagtag tatttgaaca aaaagaagat ttaagtaata atgtgagtca
     9001 ggtagggttt gtaaccttga gttacaatgg agaaaaatac gtacttggag accatttttt
     9061 agaaaatggc gaatcaatag aatatgctaa tttttatgat ttggactcag atggatataa
     9121 ggaagttatc ttattggtta aatccaaaga caaaacgaat atgcatatat ataaagtagt
     9181 agataatgaa ataacaaaaa tatatgactt ggatgcatca tggcttcaaa ataaagaaga
     9241 ttttaatgat atgaaagtaa aaattggata tatagatgga gatgacaagt tagacatatt
     9301 gatgcttcat ttgaataata agactaatga aatgtttgca agtgttgcaa attttgatgg
     9361 gaaaatgaaa atcaaagatt ctgttaagtt tgaaaatgta aaaaacttaa gtgaattata
     9421 tataacatta ggtaatgttg cttcatctgt tggaaattct tctgcagcta taaaaggtat
     9481 tgtacttgat attcctatat tgaaagataa taactatatt acgcagatac tttatatgaa
     9541 agatgggaaa attaacaaag cttttagtga ttatgataaa actattacta aatcttatta
     9601 tatacctgta gatgatattg acaaagataa gattatagaa atacctatag ttgcaggcag
     9661 tacaggtaat aataaaaata cttatagttc taaatcatct gcaactataa gttggtatag
     9721 atggaatgga aaagaaggtg ctgactcaag tttaatattt acaagtcaga tatattataa
     9781 ttataaatat aattttaaat tatttattcc aaataattta tttgataaaa tacttgttga
     9841 acaggaattt gttggagaaa aagcagtatt taaattttac tattttgact attcagaccg
     9901 taagaattta tttaatatag tagtagagag taaaaataaa cttgaagata gaaaaaatac
     9961 aggtactcaa aattctattg tacttcaaga gagcgatgaa tatactttct tactagttgt
    10021 aaacaatgca aatgaaatgg ataagctaga tatgactata gacgcattaa aagaatattt
    10081 ctcattaata tatggttaat attatactca attggaggtt ttattttatg aaggaaaaga
    10141 tacttatact agaagatgaa attggtatta gaagttttgt tagtataaac ttaaaaaggg
    10201 aaggctatga aatagttgag gcaggtactg gaagagaagc tatagaaaaa atgacaacag
    10261 aaaaggatat tacaatagct cttttagatg taatgcttcc agatataagt ggtatagaag
    10321 tatgtaaatt tataagggag aattttgacc aagtaggtat aataatgctt actgctaaag
    10381 ctcaagaaga tgataaaata gaaggattta tctcaggagc agatgattac atcataaaac
    10441 catttagtat aaaagagctt ttagtcagag tttctgctct tcttagaaga gtagcaaagg
    10501 atgattcaag tgttaagtcg agtgagatag tttctccacc atttatactt gatatagaca
    10561 aaagaaaatt atttaaaaat ggaaaggaaa ttgagcttac accaactgaa ttttcaatag
    10621 tgaaatatct aatatcaaat gctaagcagt cgttaagtag agaccagata ttagacgaag
    10681 tatggggaac taactattta tatgatttta aaatagtaga tgtaaatata agaaggataa
    10741 gaaataaaat agaagatgac ccatcaaaac caaaatatat acaaactatt tggggttatg
    10801 gatactgttt tagaaaggaa gaataatagt gttacatttt gaaggtctga gaaaaaaagt
    10861 aataaaaaac tattttataa taattattat aatggttact ctgtttgaag gattgttcat
    10921 gttttacata caaaactact attatgattc tgtcaaacag ctacttgaat ctgaaataaa
    10981 atatgctgat gaatataatg ctattacaat ggaaactaca agttttgaga aaaaagtaaa
    11041 aaatatattt gataaacaac ccttaactaa aaattcagaa tttggaattt caataataga
    11101 taaagataaa aatattatat tagaccaata tggttttaaa agtaaagaaa aagcaaacta
    11161 tgaagatgtt aacaatgcat taaaagatat aaaaactaaa aatttaacac cttatactta
    11221 tagaatacca gatacagggg agcatgttat gagtatctcc ttaccactta aggtaaataa
    11281 tataatagaa ggtgttgtta gatatacagt atcattagat gctattgata atgcaatatt
    11341 aaagcaagct acatggctta tattagcagg aatatttata cttattatag ctatactaat
    11401 aagtcttaaa tttgctgaga ctcttataaa accattacga gagctaaaga aatttgcaaa
    11461 tgaattggct gtaggaaatt ataatataaa attagaaaag atgaagattg tagatgatga
    11521 aattggtgat ttagcacaga catttgaaca catggcacat gagattgaca aaagtgaaaa
    11581 attaaaagag gaatttatat catctgtatc tcatgaacta aggactcctc ttacatctat
    11641 aaaggggtgg agtgaaacct taggttatga aagtataaca agagaagaac tagacttagg
    11701 tttaggcatt atacaagatg aaacagaaag acttataaaa ctagttgaag aattattaga
    11761 cttttcaagg ctttcttcag acaggataaa acttcatgtt gatatagttg atgttgaagg
    11821 tttaattgta ggtgttgtaa atcaacttaa agttaaagca gctgaaaaag atatatcact
    11881 tttatttgaa tttgaaaatg aatttataga gaacattcaa ggagataaaa atagattaag
    11941 acaggttctc ataaatttaa tacaaaattc atttaaattt acgagtcaag gtggttacat
    12001 aaaagttgta gcttctcaag atgaagagat aacaaccata tcagtagagg ataatggttc
    12061 aggtatagaa aagcaaaatt taaataaagt gttagataaa tttttccaag aagattataa
    12121 taaggctggt agtggattag gactagcaat tagtaatgaa attgtaaaac ttcatggtgg
    12181 aagaatgaaa atagaaagtg aaaagaatgt tggaacaaaa ataactttta atataaaaaa
    12241 taaatttgct aaacaagcat aaattcttag aaagaggatt aagatgagaa acataaaaat
    12301 gatagtagca tacgatggtt caagatataa aggatatcaa aaattaggtg acaataatat
    12361 gactattcaa gaaaaacttg aaaatgtttt aagtaaaatg actaatgaaa cagtggagat
    12421 aataggttct ggaagaactg atatgggagc tcatgcaaga ggacaagtgg taaattttag
    12481 aactaattgt atggattctt tggataaaat tcaaaaatat ttatatgaat atttaccaga
    12541 agatatagtt gtaaaaactg ttgaagaagt tgatgaaaga tttcattcaa gatacaatgt
    12601 aaaatcaaaa acttatatgt ataaaattga caataataag tatcatagtc cttttataag
    12661 aaaatatgct actcatgtaa gtaaaaaatt agatttagat agaatgagaa aagcaagtga
    12721 gtatttagta ggagaacatg attttaccag ttttgcttct tctaaatcta agaaaaaatc
    12781 aaatgtaaga gaaatttatt ctataaatat aaaagaagat gataatgtta tagaaatata
    12841 tgtagaagga aatggatttt tgtataacat ggtaaggata atagtaggtg ctttaataga
    12901 tgttggtcta aaaagaagag ctccacaaga tattaagtat atgcttgaat caaaagatag
    12961 atgtcaatca tcagatactg caccagctaa aggactatgt ctttggaaag taacatatta
    13021 atcaattata taaaactctc tttaaaataa aaataaaata aattatatag ttgacataaa
    13081 tggaaaaata aactattata tattttataa gagagatata aaactttata tttaaatcct
    13141 gtgagaaaga gagtaacttc tatgtgtttt atagagagct gaggatggtg gaagcttggc
    13201 aaataagtat tagtgaaaag aactttggag gatactaatt gaatttagta tgttagtagg
    13261 taatctcgcc ttaagagaaa gagtggataa gtttatctta tcaattagag tggtaacgcg
    13321 gatatatttc gtctcttggt ttttccaaga ggcgtttttt aatatattta attattagtg
    13381 tttatctggt atgttgaaca tttactaatt gattagctaa gtttatcaat aagagtggta
    13441 ccgcggatat ttcgcctctt agtattttaa aatactaagg ggttttttta gtattaaaat
    13501 gattttatgt gagtctaaat tctcagagtc attcaatatt aagaatttct ataaaataaa
    13561 aattataatt taagattggg aggaattaaa aatgaaggaa aaagtagtat tagcatattc
    13621 aggaggactt gacacatcaa tcataatacc ttggttaaag gaaaattatg aagatataga
    13681 tgttatagct gtttgtggaa atgtaggtca ggaagataaa atggaagatg tatatgaaaa
    13741 agctttacaa agtggagcat caaaggcata tgtggatgat ataagtgaag aatttgtaac
    13801 tgaaactata tttaaagccg taaaagcaga ggctaaatat gaaggaaaat acttattagg
    13861 aacttcttta gcaagaccta taatagctaa aaaattagtt gaagttgctc ataaagaagg
    13921 tgcaaaatat atatgccatg gatgtacagg aaaaggtaat gaccaagtta gatttgaagc
    13981 tacaattgca gctcttgacc cgactataaa agttattgca ccttggagaa tttgggatat
    14041 aaaatcaaga gaagatgcta tagactatgc tgaaaaacat aatataaagg taacagcaac
    14101 taaagctaaa atatattcag tagatgctaa cctttggcat gtttcaacag aaggtggaga
    14161 tatagagcac ctagaaaatg aacataaaaa agatgtttat aaacaatgtg tagacccaga
    14221 agatgcatgt gatgtagctg aatatgtaga agtttacttt gaaaaaggtg tacctaaaaa
    14281 ggtaaatggt gaagaattat ctccagttgc actgattcat aagttaaatg agttgggttg
    14341 taaacatgga attggtgtta tagatatagt tgagaataga cttgttggta tgaaatcaag
    14401 aggtatatat gagactccag gaggaacaat cctttatgaa gcacataata ttttagaaag
    14461 tgctacttta gacaaagata cattacattt caaacaaatg gtatcttata aatacggaga
    14521 acttatttac aatggactat ggtattgtaa attaagagaa tctatagatg catttatgga
    14581 acaaactcaa gataatgtta caggaacagt aaaagttaaa ctttacaaag gaaatataaa
    14641 acctgctgga atatttactg aaaatgctct ttatgatgaa ggaatatctt cttttggaaa
    14701 tagtgaactt tatgaccaca aagatgcaga aggatttatt aatttattca ctttaccatt
    14761 aaaaatcaga gctatgaaag ctggaaaata ataaaataaa ttatttaaga gtgtataaaa
    14821 actattttta ggtttttata tgctcttttt ttatttgaat ttattaactt atacagtaaa
    14881 tagtataaaa aaatatatta tagttccaaa tggcttaata atgtgaaaat ataagaccta
    14941 aaatataaat tttataaata caatagttat tgactctctc attgtaagag ggtttatact
    15001 gaatctaatg aaaatttata gtgaggagat agattatatg agaaaaataa ttgaaattaa
    15061 acatcattca catgattatg agtgtatgtg gaatggtata gaagatttat atataaataa
    15121 aaccggtgaa tgccttccaa acgatttttt ctttttactg tcaggttttg gttcattttg
    15181 ttatatgaaa acaaataaag ctgatttgaa aagaatggta gctttgggag atggaaggac
    15241 aaaacagatg tataaatttt tagcaccaat tgttggattt gaatacaaac atcatgagtt
    15301 taaaaagtat gagcaagcat taaagaaagc aaaatctgaa attgatgatg gatatccagt
    15361 tattttagga gctttagata tgtattatct accatattat aagaagctat atcataaaga
    15421 acatattcct tttcattata tattaatggt aggatatgat gatgaaggaa tatatctgta
    15481 tgattgtggt agaacagaat tattacatct ttcttatgat aaattacgtg aaagcatgaa
    15541 ttgcaattcc cctggattaa gcaaagaaaa tactatttgc acaataagaa tgaatacttc
    15601 aaagaataaa tataacattg ctagagaagc tattgctata aagcgtgata tgtttttaaa
    15661 tccacctaaa ggatttttgg gatataaagg ttttgaaaag tttattaaag agttgccaca
    15721 gtggaacact gaattggata aagatgatta tgataagatt ttgaccaata tggttatgtt
    15781 ttttggaaca gtaccaacaa ttccaaatgc aatcaaagga attaaagaac cagatactgt
    15841 tcaatttaaa ggtggatttg ataaaatgag tagaatgctc aacagtattg gggaggaata
    15901 caaaaacgac tcttttatac aagtagctga tatttttgat aaaactgcta ttataattga
    15961 gaaaatatcc aatattataa tagattactt aactcaaaca tgtgatgata caggacaact
    16021 tccgttattg ttctctgagg tcttagagca tatgaaaaca ggatatttaa tcttggatgt
    16081 ataaaaaata tattgttgac cctaacatta cgtaatagtg taatatgaaa atatagcttg
    16141 aatatattag taggtattga tggctgtaaa attaaaattt gaggtgtgga tatgagaaca
    16201 gtgaaacaag tttctgattt aactggaata agtgtgaggg cattacatta ttatgatgaa
    16261 ataggtcttt taaaaccaaa taaaattaca gatgcaggat atagacttta tgatgatgaa
    16321 tctattaaaa ctcttcaaca aattttattt tttaaggaga ttgatattcc tttaagagag
    16381 gttaaggaaa taatgtcaag ccaatatttt gataaggttg aagctctaaa aaatcaaaaa
    16441 aaattactta ttttaaaacg aaagagactg gatgaattga tagagcttat aaatcaaact
    16501 ctaagaggag aaggaaatat tgattttaaa gagtttgata tgagcgagta ttttgatgtt
    16561 ttagaagaat ttaaaagaga gcataggaat aaggtaatta aaatttatgg tagtgtagaa
    16621 aaatacaatg aatatataga aagagttaag tctaatgaag agaaaattgc taaaatggct
    16681 ataaaacaat atggaactat tgagaagttt gctaaagcta taaaaacaaa ttttagcagt
    16741 gatatattga atttaggaga aaaatttgat agatataaaa atgattgttt aaaagaaaaa
    16801 catccaaaat taaaagaatt atatagaaag cttgtagaag atttaaatag gaatccttct
    16861 tcaacagacc ttcaagaaat agcaaaagag ataacagata tatctaaaaa agattatgaa
    16921 attttctcta tggatactgg tgatgacaac tggtattata tggttcaaaa ttatctagta
    16981 aatcctatgt ggatagaaga agtagataaa aagtatggaa gtggagcagg taagtttatt
    17041 ggacaagtac taaagactta tctaagagat agaaaaccaa agataaatac attgtatgaa
    17101 aaacttgtag aagatttgag tagagattgt tcttcaaggg aagttcaaag cattgttgaa
    17161 gaaatagaca atgaaatgaa aagaagtaat gaattttata aaatagacaa tggtgaaaga
    17221 tactttgatt atatgtcaga gctttattta caagattcta actatataaa agtaactgat
    17281 aagaattatg gtgatggtgc atctaagttt attggagaag cttttaagat ttattttgat
    17341 aataataatt gttgaaaatt tggaagtaaa aaatatgcaa gttttataaa agcacaaaaa
    17401 taaggatgta gattagctcc atctatatcc ttatttaaag ttattaaaat atatagttta
    17461 catcaaactt aacactttca ataatatttc ccaaatatgt ttttatactt tcaacttctt
    17521 ctaaagattt taatttattt tcagtataaa ttgctttgtc cagaatatta aaccttaatc
    17581 ctatttcatc aaagtactca tgatttaaaa ctttgtacca atggtttttt tctttattcc
    17641 aactcgtaga tattgaatca gcttcttttt gagctttttc ccaatcttca ttttctatag
    17701 atttttctaa aacagatata ttgtctttat atccttctgt aaaatcatat agtttattgt
    17761 taacataaaa tccaaataat acaaataaga tagtccatag tataacaaat gttgtagatt
    17821 tcaatattaa tcaccctttt tattttgaat aaatattttg tcattttcat ctaaaacaca
    17881 aataagaaca tctttaaaag tttctatgtg attagatttt aaaataccca ttaaccagtt
    17941 agtatctttt tgagctatgt ctagattttt atttattatt ctaccatcca gtataagtgg
    18001 gataggtaaa tgattaaaag ctcttggagg agtactattg tagcttgatg ctggtacaac
    18061 acttagatta ccatcagtct ccaaaatagc atattgtaca tcttttaggt taaagtatcc
    18121 ttgaatccta agttgttcta ataattcatc tatactaatt ctttcttttt taagctcttt
    18181 atatacaatt tttcctttat ctattaatac agaaggttta cctgataaaa atgaacttaa
    18241 ttttctactt tttaatgata agaatgaaat tagagtctgc atgattacaa gtccagatat
    18301 tgctgcaaca ccatttatga ttggtatatt attattttcc attgggacag atgcaacttc
    18361 tgcaattaat agtgttataa ctaaatcaaa acagttcatt tcagctattt caccttttcc
    18421 catgactcta agtgcaatta gcactgttat gtataatata atacttctta ttaatactac
    18481 tgtcatatct atcaattact cctttaaaaa atatttacac ttagttagta tatcctatat
    18541 aaaaaattta attcaaagta taaaaaacct taaataattg gattcaacta tttaaggttt
    18601 ttttgataat tttaatatta aattttttat ggattttctg ttacttttat ttattttaat
    18661 aacagattca acaaaactct ttttatctcc taattttaag ataaaatcag gatattcttc
    18721 aaattcattg agttttatta atttgaaaga agaagataga tttttaaagt taataatttt
    18781 agagttattc actaatacac ttcttttttt aaaataaatt tcttttttac ttatatttag
    18841 tatagttaat atttgtttaa gatatattat tatatcttct ggagttctat caaatttgag
    18901 ttcaaaattt tcatcttcaa agtagggaat ataatataaa ggtgttttga gagcaggtaa
    18961 tttattatat aattcttcaa gaatatcgat atttttaggg gagttaaaga aaaagcattt
    19021 acatacattc attaaatcca cagcatctga aagtgagtta tgtttaatat ctccttctac
    19081 agaaaaaatt tttttcatat tcagtaaaga tatttgctta gataagattc tattattgta
    19141 tgtaatatgt tgagatataa aaggttgaat gtcaatcatc atattaaata actctaagca
    19201 tttacctgta tatttatata ctctaaatga agttagaagg ccagttatat caaaatcacc
    19261 ccaattatat attgtaacat catcaaattt acaaaaccat ttataaaaat catccataac
    19321 atataaaaga ttaggagcat tattgatatc tagttggttc aatttggtta aatttttaca
    19381 tttatttgaa agtattttat tgtattttgg cttaactaaa gagtaataat tatctattgt
    19441 attaaattcg ttatcaacta aaactccacc aatagaaatt aattccgcac cattatattc
    19501 ttttggttta tttttattat catctagtat attaaattca aagtctataa ataacttgta
    19561 tttcataaat ctaaagcaca tcctcaggta aattatttat tgagcctaag catcataaga
    19621 atcaatagat ataatgactc taaacatcaa aggttcatta caacttttat ttatttcata
    19681 atttaattca aataaatctt ttaagatata atttataagt attctttccc aacccatttt
    19741 taaattaact ttagagtcta ttaatatatt ttctatcttt ttaaatattt ctatatttgc
    19801 tggtattata ctgagctcaa tattactttt aaacgggtta gactgtaaat ctgttataag
    19861 tatatttttt aattcctctg taagttcaga aattaaacta tcacctatgt atttttttat
    19921 gatttcctct ctaatttctt ttaaatttag caatattagt acctgctact tacatagtag
    19981 cttttccttt ccttttaata ctattattca tttttgaatc ttatttagat atattttaag
    20041 attaaaagtc tttagctata taatttttta cccattttta acttatatta acatattaca
    20101 ggcttatatc tccaccctgt ttacattgag gatggagtat attgcctatt ttgataaaaa
    20161 tgataggttg ctatttgcct attccttttt ctgataattt ttctaacata tctttggtca
    20221 ttttatctaa atcataacta aacttaaagt tccattcttt tactgcacta ctactgtcta
    20281 gagagttagg ccaagaatca gctatacttt gtcttacagg gtctacatca tattctatca
    20341 caaaatcagg aatatatttt tttatagaat cagctatttc ctcaggagaa aagctcatag
    20401 cagtaatatt aaaagcattt ctatggatga gtttatcagc tggagcttcg attaaatcca
    20461 ctattgattg aagtgcatca ggcatataca tcatatccat ttttgtacct tctgctatgt
    20521 aagatttata tctcttattt tttaatgcct cataatatat atcaactgca taatctgtag
    20581 ttcctccacc aggaggcgta acataagaaa taagacctgg aaatctaaca cctctagtgt
    20641 ctactccaaa ttttgagtga taataatcac atagtaattc tccagaaact ttagtcacac
    20701 catacatagt attaggtctt tgtaatgtat cttgaggagt catatttttt ggagaatttt
    20761 caccaaaagc agcaattgaa ctaggtgtaa aaaatttaca atccaattct ttggctattt
    20821 caagaccatt tattaatcca ttcatattta agttccaagc ttctagaggt ttactttcag
    20881 caactgcaga aagtaaagat gctagatgaa ttatagtatc tacatcatat ttttttgcaa
    20941 tttcaaaaaa acgattctta tctgtaacat ctaatatctc aaatatacca gattcacata
    21001 ctggattacc ttcttttact ctccttgaac tagcgattac attttgttct ccatactcat
    21061 ttctaagctt tattgttaat tctgaaccta tttgtcctaa tgcacctgtt ataagtattt
    21121 ttttcaattt tacaacctcc taagggttta atttatgtag tactacttat ttttatttta
    21181 gcaaatcttt tgtttaaata caaagaatta aagataataa tatacgactt aattgaatga
    21241 agtgcagtaa gtgcctaaaa atttaaagct aatatataat ggtatattgt ataacaataa
    21301 aagagtagat taaattatta tatatagatt gtgtgactaa gtcacagaaa atagagttaa
    21361 tttaatatat aataaaagca tatcaagtaa agctttaaat taaagtaact ttataaataa
    21421 aaagtaatat aaattgataa aattataaat ttatttatag tgaatatggg ttggatttta
    21481 tagatacaaa aatagtatat atgatatgta gaccattagc aggtttaaag tagcaaaaca
    21541 tataaaataa aatttgaaag gaagtataaa aatgacaaga ccatcaatat atcatagcca
    21601 aggatacact tgtgcagagg cattaataaa gtcttataat gaggaacata acacagatat
    21661 accaatctca ataggaagtg gtatgggggt tggaatgaat gttggaagtg tttgtggagc
    21721 tgtaaatgct gctgttgtta taatagggta tttaaatggg cgaaatagta atttagatga
    21781 aaatatagca aggaagtatt caagagagct attgaataga gtaagaaata aatacagctc
    21841 agaaatatgt gtatctctta aaaaaaataa agtaacttgt gaagaaataa taaatttcac
    21901 atatgatgct ctaaatgata tgctaaaaaa tcaaaaataa ttctaagaaa caagtatttt
    21961 acattcgata gaatacttgt ttttttatga tatttttaat aagtattagt ttattataat
    22021 ttttaaatca attttattat attttaattg aaacaatcta ctttgaaaat tactaactga
    22081 caaaagaata tgattatagt atagaaaaaa tgtaaattat ttaatattaa gtgagagtat
    22141 atgtttacct atataaaatc gtataaatta atggctaaat ttataaaata taaagaaata
    22201 tataggaaaa caaaagaatt aattagtata atataaagtt taaaagttaa aatttatata
    22261 atttatttat aaagtaaaga aaaaatagaa atttgtaata aaataaaaag tttatgacat
    22321 aatatggtat aattagtaaa aatatagaga ttggaggaat tgctttgttt atagatgaag
    22381 aattagaagg ttatatatta acatgtaaaa tatctgaaga ctttaaaaat atacctgaat
    22441 atagtgatga agagttttat gttacagtct ataaagatga aagttctgac tctgggtact
    22501 atgctttatt agaaaataaa gaagaaagag ttgtatggga tggagaagtt gttgccaata
    22561 atatttttaa taacctttgg attgtagtaa ataaggttaa aactggataa tagtctaatt
    22621 atatagtatt tgtaatattt taaatgaatt tttacatatt tgagatgagt aaataacaaa
    22681 gattattcga tactggagga gtaattcaat ggaatatagt tatagtaaga tgaacttaaa
    22741 aaaaggggat attgtagagg taaatttaga gaaacaagcc aatgtaatat tgttagacca
    22801 tataaattat gtgaaattta aaaatcaaag aaattatgat tactatggtg gatttgcaaa
    22861 gaaaaatcca tgtagaatga gagttcctaa tactggaaca tggtatctag tagtaaatca
    22921 agatggaaat agtggaatag taaacttttc aatcaataca attcaaaatt aattttagaa
    22981 tattggatat tcttaatttt tgtacatttt atatagttaa aataaaatta ataagcaatt
    23041 attataaaat aatgtgggtg cctcaaaatg aattttataa ttcatgagac actctttttt
    23101 atatgaactc aaatatactt tatcatttca acaatgaaaa agaggcgtat ctgtgttttg
    23161 agatagcctc atttatcata tatcatatat agaattttaa ttattcttaa ctaaaatttt
    23221 ttccataggt tatctgtaca attaaaatat ttattgttag catacagctc cacctgtaag
    23281 gtgtaaaaga gcctctagta tctcttcttt tctttccaaa acttcatctg ataaaagcat
    23341 ttctataaat ttatcagcac cgattctatc gatagttatt ccaaaacgtt cacctgtgtt
    23401 accctgttct ctaaataaaa gaattgcttt ttcaattaaa gtcataagtt cctcttttga
    23461 aaataatcta tcaatctgtg ttccaggacg aactgatttt ccccatttcc ctcctatata
    23521 gactttataa ccagattctt tttcttcaat actatcaaaa ttacaacttt ctatacactt
    23581 accacagtta ttacatagat tactatctat ttcaagttta cctttatctg ttaatttagc
    23641 tgctttaaca ggacagacct ctacaacagc acatttttta caaccaacac aaagctctga
    23701 atcatagtca ggtacacgtt gaccaacaat tccaagatcg tttaggtcag gtttaataca
    23761 gttattaggg catccaccaa caccaatttt gaacttatga ggtagtttta catcatacca
    23821 atttttgtaa aattcttcat gtatttcagc agcaagtgcc tgtgtattag ctagaccatg
    23881 aacacaaaca gtacctttac aagccacaac aggacgaacc cttgatccag ttccacctgt
    23941 tattagattt tcgctagcaa taaattcact tagagcttca atatcttcaa attttacacc
    24001 aggtaattct actgttagtc tagtagtaaa tgctaactga ccgtttccga atttttcagc
    24061 agcttctgca attttttttg cttgtgatgc atttattaca ccatttacag ttattatacg
    24121 tgctgcaaaa tgttctccat ccctgcttgg tagaaaacct ctacctttta cttccttttt
    24181 ttgttctgct gtaacattac tcatatttac tcctccatta ccttaagatt tggataaaat
    24241 agtattccac cttcaagaac tttcgtattt gtatagccaa agcgtcttaa gcgattttgc
    24301 gccatatagc tatttttacc tttagcacat ataagaagaa ttttttcatc cttagcaaga
    24361 ccttcaattt ctccattaat ttcaccaaca ggaatataac gtaaactagg aatagaagga
    24421 gcttttgaaa catcaagttt agtccaactg tcaaaatcct caatttcgtc aagtaaaaca
    24481 ctatcaagtt ctccattaag tttattttgt aaaacattta ctgccactac aaaagggtga
    24541 atagctgttg aaaaaggtgg tgcatatgca aggtccatac tttctaatga ataaaggtca
    24601 gcgcctaaag ttattgcagt agcagcgata tctactattt tatcaaccgc tccaggtccc
    24661 ataacttgaa cacctaataa ttttctagta tttttttcag caaccattct tataataaaa
    24721 ttagatgcac ctggataata atgagcttta tcatcagtag ctatggttac agagcaaaca
    24781 ttataacctt ctttttcagc tatttcttta cttagacctg tttttcctgc attgagctca
    24841 ggtaatttta ctactgttgt tccaagaaca ccattgtaag ttttagattt tccagatata
    24901 ttttgtgcgc aaattctacc ctcatgattt gcagatgacc ccataggtga ccatgtaggt
    24961 ttatttgtta aagaattttt tacaaaagca cagtctccaa ctacatatat atctttgtca
    25021 tttgtaagca tatattcatc tgcaataatt gtattattag gggcaaattc taaacctgtg
    25081 tcttttagaa aacctgtgtt aggacggatt ccaactgaca taaccactaa atctgtttta
    25141 atagctctat ttttagtacg aagtttttca actttatttt caccttcaat acctacaacc
    25201 tggtcacctg taaaaatcat tatgccttta tcagccatat gattttcaac gtattcagca
    25261 aaatcagtat caaatccagg cataacatgt tcagccatat caacaacact tacacgtata
    25321 cccattgcag aaagattttc agcaatttca agaccaatga aacctccacc aataacaaca
    25381 gctctcttaa taccgttttc aatagaatct cttagtttta tagcatcatt tggagtacgc
    25441 ataaagaata ctccaggtaa atctattcca ggtattggtg gttttattgg gtctgcccct
    25501 gtagcaatta caagtttgtc atagcttaga ttttcttttt ctcctgttga aagcgttttc
    25561 aaagttactt gttttgcatt tctatcaata gatgtaactt caactccaca acgaacttct
    25621 gctcctgtta gtttagaaaa actttcaggt gtatttacga ttaaagatga tttatcttca
    25681 attatcttac caacgtaata tggaagacca caacctgcat aagaaatatc atcaccttta
    25741 tttagtattg taacagagca attatcaccc atttcacgtt ttatttttgc agcagttttt
    25801 gttccagcag caactccacc tataattaat actttcacaa tatatcacac tccaatctac
    25861 tttataactc tataataaaa ttatacttct tgacaatata tttgtaaaat actaaaatat
    25921 taataaaatc ataagcattt actaatgttt gctaaggttg tgataatatg acaattcgta
    25981 gtcttgaaat ctttgttaag gttgctgaat gtggtaaaat gagtgaagta gcaagaaata
    26041 tgtatattac ccagtcatca gttagccaag ctatttcaga aatagaaaaa gagtatggtg
    26101 ttaagctatt tgatagaatt tcaaaaaaat tatatttaac tgaggcaggg aaaaaacttc
    26161 ttggatatgg gaggcatctt ttagctgtaa atgaagaaat gaatgattgt atgaagcatt
    26221 gtgcaaaaaa tatccgtata agagttgggg caacagtaac tgttggtaca tgtgtaataa
    26281 gtccaataat gttagaatta tacaaggtaa atcctcttat agaacctgaa gtatttgtag
    26341 aagatacaag actgattgca aaaaaattac ttaatagtga attagatatt gcgattgtag
    26401 aaggaaaaat taagcaccca gatattgtta caaaaagcat tattaatgat aatcttgtac
    26461 ttatatgttc acacaagcat gagttttata aaagagactc tataaaagtt tcagaacttt
    26521 caaatcaacc attaattatg cgtgaattgg gaagtggtac aagagcacaa ttagaaaagc
    26581 aattaaaaga attaaaaatc ccaatgaata ttagatggtc atgttataac tctgaagcta
    26641 tattaagagc agttgtagat aattttggca ttgcagttat atccgaatta cttattgagg
    26701 attatttaaa aaagcatctt ttgtgggctt gtgatattga aggtattaat ttacatagga
    26761 catttgatat tgcatatcat agaagcaagt tttttacaga aaatatttca gcattttttg
    26821 atatttcagt agaatatggg aaaaaacaag cgagaaagaa gagtagaagt gtaaacagtt
    26881 tataatatat ctaaataaaa ataagagtat atcaatatac tcttattttt atttagatat
    26941 attagcctat attaacgcca aaatttttac acaatcctgc aagtccatcc tcaaatccag
    27001 aacctaaagc attaaatttc cactctccat tatgtctata tagttctgcc acaactattg
    27061 ctgtttctat gctaaaatct tcacccaatt catatttaat aagttcctca ttagtttctt
    27121 cattatatat tcttatatat gaattttcaa cttgtccgaa attttgtctt cttgtaattg
    27181 cttcatttat agtaacacta aatgatattt tagcaatatt ttgaggtatt ttagataagt
    27241 ctacatttat ttgttcatca tcaccatcac caacacctgt tctattatca cccatatatt
    27301 caacagctcc agaagcgtgt tttaaattat tatagaatat aaagtctcca tcattcgtta
    27361 cttttccatc agttccagtt aaaaatgcag aggcatctaa atcgaaatca aagccaccat
    27421 catatttatt aatatcccat cctagtccta ctattacttt ttttagacca gggttacttt
    27481 ttgttaaact tactttttgt ccttttgcta aagttatacc catttaaacc aactcctcat
    27541 tataaatttt ttattgtaat tattacttat atgtattaat taattcacga agctcttcat
    27601 tttgaccaat tcctaaagca ttaaatttcc attcttcatt tctacgataa atttctcctg
    27661 ccaaaactgt tactttacca gaaaagtctt cttgaagatt atatttaaat aattcttgct
    27721 tggaatttga atcaattaat cttataaaag agttgtttat ttgaccaaaa tcctggtctc
    27781 tttttttagc tttgtatata gaaactccaa aaactatttt atgtacatat tcaggtatgt
    27841 cttctaaatt aacaattatt tgttcatcat caccttcacc ttttccagtt aagttatctc
    27901 cagtgtgtat aatagcttta gaagggtgtt taagatttcc aaaataaacc aagtcacctc
    27961 taacattcat tttatcttgg ttagtcaatg ctattgcaaa tgcatctagg tcataatttt
    28021 ttttacccaa tttattcata tcccatccta agcctactac tagatttttt acaggattat
    28081 tatcttttac taagctaact ctttgtcctt tttgtaaact aacactcatt ttaagttctc
    28141 ctttttatat ttttagtttt attgatggta tcaagtataa gaaccatatt agctatatta
    28201 tatacgtatt taggctaagt tggctatatt tataaattat ttattatata actacatgaa
    28261 tattttataa tttaaacata ttgacattat atgctttaaa ttattttgta gttttataat
    28321 atttaaattt gtatttgatt gttataaagt aaagtaagta ataaagttag actcaataat
    28381 tctaaaattt ctaagcatac tatctaaatg agaacggatt tggaaatact tgtataaaac
    28441 aataattgat acatttaatt tttatcttaa aaa
//
LOCUS       DAYASV010000039        27916 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYASV010000039 DAYASV010000000
VERSION     DAYASV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.039"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(183..938)
                     /gene="dapB"
                     /locus_tag="ACGI68_001679"
     CDS             complement(183..938)
                     /gene="dapB"
                     /locus_tag="ACGI68_001679"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFH0138121.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHETLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            1378..2367
                     /locus_tag="ACGI68_001680"
     CDS             1378..2367
                     /locus_tag="ACGI68_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFH0138122.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            complement(2819..3535)
                     /gene="dapD"
                     /locus_tag="ACGI68_001681"
     CDS             complement(2819..3535)
                     /gene="dapD"
                     /locus_tag="ACGI68_001681"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFH0138123.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(3759..4508)
                     /gene="dapB"
                     /locus_tag="ACGI68_001682"
     CDS             complement(3759..4508)
                     /gene="dapB"
                     /locus_tag="ACGI68_001682"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFH0138124.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(4575..5462)
                     /gene="dapA"
                     /locus_tag="ACGI68_001683"
     CDS             complement(4575..5462)
                     /gene="dapA"
                     /locus_tag="ACGI68_001683"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0138125.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(5579..6580)
                     /locus_tag="ACGI68_001684"
     CDS             complement(5579..6580)
                     /locus_tag="ACGI68_001684"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFH0138126.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     regulatory      complement(6753..6927)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(7005..7886)
                     /gene="dapA"
                     /locus_tag="ACGI68_001685"
     CDS             complement(7005..7886)
                     /gene="dapA"
                     /locus_tag="ACGI68_001685"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0138127.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            8209..9402
                     /locus_tag="ACGI68_001686"
     CDS             8209..9402
                     /locus_tag="ACGI68_001686"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFH0138128.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            9608..10726
                     /locus_tag="ACGI68_001687"
     CDS             9608..10726
                     /locus_tag="ACGI68_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HFH0138129.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            10857..11102
                     /locus_tag="ACGI68_001688"
     CDS             10857..11102
                     /locus_tag="ACGI68_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0138130.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            11089..11835
                     /locus_tag="ACGI68_001689"
     CDS             11089..11835
                     /locus_tag="ACGI68_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0138131.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            12120..12995
                     /gene="hslO"
                     /locus_tag="ACGI68_001690"
     CDS             12120..12995
                     /gene="hslO"
                     /locus_tag="ACGI68_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFH0138132.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            13386..14186
                     /locus_tag="ACGI68_001691"
     CDS             13386..14186
                     /locus_tag="ACGI68_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0138133.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            14714..16954
                     /locus_tag="ACGI68_001692"
     CDS             14714..16954
                     /locus_tag="ACGI68_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0138134.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            17368..18903
                     /locus_tag="ACGI68_001693"
     CDS             17368..18903
                     /locus_tag="ACGI68_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0138135.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIVIIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            18919..19680
                     /locus_tag="ACGI68_001694"
     CDS             18919..19680
                     /locus_tag="ACGI68_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0138136.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            20018..20887
                     /locus_tag="ACGI68_001695"
     CDS             20018..20887
                     /locus_tag="ACGI68_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138137.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            21216..21467
                     /locus_tag="ACGI68_001696"
     CDS             21216..21467
                     /locus_tag="ACGI68_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HFH0138138.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            21579..22358
                     /locus_tag="ACGI68_001697"
     CDS             21579..22358
                     /locus_tag="ACGI68_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0138139.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFINYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(22493..23143)
                     /locus_tag="ACGI68_001698"
     CDS             complement(22493..23143)
                     /locus_tag="ACGI68_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138140.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDSTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(23140..23643)
                     /locus_tag="ACGI68_001699"
     CDS             complement(23140..23643)
                     /locus_tag="ACGI68_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0138141.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(23692..24207)
                     /locus_tag="ACGI68_001700"
     CDS             complement(23692..24207)
                     /locus_tag="ACGI68_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138142.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            24487..25014
                     /locus_tag="ACGI68_001701"
     CDS             24487..25014
                     /locus_tag="ACGI68_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0138143.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            24998..26401
                     /locus_tag="ACGI68_001702"
     CDS             24998..26401
                     /locus_tag="ACGI68_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0138144.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(26784..27095)
                     /locus_tag="ACGI68_001703"
     CDS             complement(26784..27095)
                     /locus_tag="ACGI68_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138145.1"
                     /translation="MKKIIKSFTSWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(27151..27744)
                     /locus_tag="ACGI68_001704"
     CDS             complement(27151..27744)
                     /locus_tag="ACGI68_001704"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0138146.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT        10333 a         3448 c         4556 g         9579 t
ORIGIN      
        1 ataaaataat aaacaatata attccatata ccaataaata agataatctt taagaaaaat
       61 gattatattt aaaaggtaaa cataaaattt ataaactttt agaatagaaa tctcaatcaa
      121 ttagatattc aaattgatga atatgaatac ctaaatagat tgagatttat tatgtacaaa
      181 acttatccta atatatctga cattttataa agcccagatt gcttaccaat taaaaattta
      241 gctgcaacta tagaaccgtt tgcaaatact tcatcagatt gagaatgatg gcttaatgta
      301 agaacttcat tatcacctgc aaatacaaca tcatggtcgc tgattatatt gccacctctt
      361 atagagtgta ttccaatttc atttggtttt cttttacaat ctctaccata tctaccatgt
      421 gtattttcac tttcaggcaa gacttctcta attgcattag ctatcatagt agcagttcca
      481 ctaggggcgt cttcttttct attgtgatgt ttttctatga tttcaatatc aaaaccttct
      541 aacatctttg aagcatcctt aactaatttt attaatatat tcactccaag agatgtattt
      601 gatgataata atataggaat gttttttgat gcttcttcta ttttatttaa gtcattttca
      661 tcatatccag tagtagctaa tactacaggt gtattatttt ttagagcata tgacaatatt
      721 ggctctaaag tctcatgatg tgaaaagtct attattacat cagttttttc agttacatca
      781 gtcattgttt tgtaaagctt tgtatcacta tgatgacctt tttcatcagc agacctagct
      841 acaccacaaa ccaactctaa ttcattatct ttttttacac attctgctaa aactcttccc
      901 attgttccat tatagccact aattataact ttcaacataa attcctccta tgaaattaat
      961 aaaaatttgt tatatttcta taagtttatt ttatcactaa tataatttat ataaaataat
     1021 attagtgata ataagcttac aatttatctt acattaaaaa atttaagtgg atttttcaaa
     1081 atatagataa atttcttttt cattgtataa atgataaaaa tatatttgac ttcataccaa
     1141 aaagagtgcc ccatgaacta aaaagttaat tttgaaatac catattcata tatcatatga
     1201 tttatttgga aatcaatatt aatacattaa aataactaaa tacatgtatg cataaagatg
     1261 tataaaaaat atataaacat atagtattaa ttttaaattc aacaatttaa ttgttaaaag
     1321 tgttattatt aaattagaaa tatcttaact taatatactg agatgggagt gatagatatg
     1381 ttaaaagaag aaaaaactta tcaagagaaa gacgcaaaag tattcaatgg agtggcagct
     1441 ttgataatga ttttattatt tttagtagtt tcaataggta tcatggtttt tgggatagta
     1501 aaacttgata gtggatttac tggaataggt tcacttatgt taattttggg acttgtattt
     1561 atagtagtag attttatcct attttttggg ctaaaaatga ttaatcctaa agaagctata
     1621 gttctagttt tgtttggtaa ctattatgga actataaaaa aagaagggta ctattgggtg
     1681 aatccatttt gttcagcaat aaacccagca gtatcaagaa ttaatgtatc gaaatcttca
     1741 tctgatgaaa aggtagatat atcaactaat tcaaggggca aaaaagtatc tttaaaaaca
     1801 atgactctta ataatgaaaa gcagaaagtc aatgatttat tgggaaatcc aattataata
     1861 ggagtggttg taatttggaa ggttatagat gcaacaaagg cagtatttaa tgtagataat
     1921 tataatacat tcttatctat tcagtgtgat tctactattc gcaatgtttc aagactttat
     1981 ccatatgatg tatctgaaga tggagatgaa aaatctcttc gtggtagtag tcaggaaata
     2041 gcagatagat taaaagatga attacaatca agggttgata ttgcaggaat tgaagtgtgt
     2101 gaagtgagaa ttactcatct ttcttatgca ccagagattg cagcagcaat gttacaacgt
     2161 caacaagcag aggctataat agcagcacgt aaaaagattg ttgagggggc tgtaagtatg
     2221 gtagagatgg cattgactaa tttaagtgaa aataatgttg tgactttgga cgaagaacgt
     2281 aaagcagcta tggtaagtaa tctgttagtt gtgctttgtg gcaataaaga tgcacaacca
     2341 gtggtaaaca gcagttctat ttattaaatt ataaaaagtt taatagttta ttttattgat
     2401 atatagaaaa tttagtagtt cattttatta aattatagaa agattaatgg tttgctttat
     2461 tgaattataa aaagtttaat aatttattct attgatatat agaaaattta gtagttcatt
     2521 ttattaaatt atataaagat taatggtttt cactttatta aattataaaa agtttaatag
     2581 tttattacat tgatatattg aaaaattgaa ggaaataaaa atacttgtca gcaagaaata
     2641 aaaatgatat aagaatgaat aagagaattg taaaaaaata aaatgattat aaataaaaga
     2701 taaaaatatt gtaaaaaaga gggtgcctct ggtgaattaa attcacacag agacaccttt
     2761 ttaattaatt ttttaaatta gtatattatt gtttaattaa tagtatagta ctatttaatt
     2821 aatctaaatt tctaaggtct tccataagct tagttttgtc agccgttttt tcatctttca
     2881 tttttataac tttagcagga gaaccagcaa caacggcacc agcttctaca tcagtagtta
     2941 caacagcacc agctgcaaca acagcacctt taccgattct aacaccttca agaataactg
     3001 cattagctcc tattaaaaca tcatcttcaa ctataacagg agtagcagaa ggtggctcta
     3061 aaacaccagc aacaacagca ccagcaccta agtgaacatt tttaccaaga gttcctctag
     3121 ctccaataac agcattcata tctaccatag aaccttcacc aataacagct cctatattta
     3181 taacagctcc catcattaca acagcatttt tttctatagt aaccatgtct cttattattg
     3241 cacctggctc aattcttgca tgttcactta aagtattttt tagaggaata gcagaatttc
     3301 ttctatcata ttctacatga gtatctgtta tatcatttcc atgtttatct aatacttcca
     3361 ttatatcctc atagtcacct acaaatatat aagaaccatt acttccgaat accttaaatt
     3421 tatcagttga ttggaagtgt aaagagttag tatttacata taccttaaca ggtgttttct
     3481 ttttaacttc ttttatatac cttgctattt cataagcatc gtttaaattt accatttgtt
     3541 aattctcctt gtatttcatt atatttcttt tattatatac ttatttgtca attttagtat
     3601 atacttactt cttattattt atattttatt ttagttagtt tttattgtat agacatagta
     3661 ttaaataaat ttattatata tgtttgattt gtattgcgta ttattatatc tatattgcat
     3721 atgtttaatt tatattgtgt attattatat ttatgctatt atcccaacat gctgtccata
     3781 ttataaaatc ctggtttttg cttaactaag tatttggcag cagctataga accacttgca
     3841 aagataccac gagattgagc ttgatggctt attgtaagaa cctcattgtc tccagcaaat
     3901 agagcatcat gttcgccaac aatatttcca cctcttatag catgaatacc aacttcattt
     3961 ggttgtcttt ttgcactacg accatatcta ccataattat tttcaacgtt tggcaaaacc
     4021 tcttttatag cgttagcaat cataacagct gtgccacttg gggcatctac ttttctgttg
     4081 tgatgttttt ctattatttc tatatcaaat ccttctagta atttagctgc atctttaacc
     4141 aattttacta atacatttac accaagagac atattatatg attggaatac aggaataact
     4201 ttagccgctt cacgtatttt gttcatttcg tcatcattat atccagtagt agcaattact
     4261 aaaggtgttt tagtctttaa aacgtaagat aagatattat ctaaattaga gtgatgagaa
     4321 aaatctataa ctacatcagc ttcctcaact atagtactca tatcttcata aattttaagg
     4381 tcgccttcat agctatccag acctctagct gcaacacaaa ccaattctaa ttcattatct
     4441 tctcttatag tatcggctaa aatcttaccc atttttccat cataaccagt tgctataact
     4501 tttaacataa tttcctccta tgattaaggg ctaacttttg accaaactta ctttttacca
     4561 tgcattaatc tttactatgc attaactttt agcccaaagt taactaattc tttctttaat
     4621 acttctaaat ttgctgggtc catttctgct agaggtaatc ttaaatctcc aacattgaat
     4681 cccaataaat tcattgcagt cttaactggt ataggattta cttctataaa taaagctgcg
     4741 attagagcat ccataccaag ttgtaatttt ctagaacctt caatatcgcc ttcaaagaat
     4801 ttagctacca aatcgtgagt ttcttgaggg caaacattag caagtactga tataactcct
     4861 tgacctccta aagataataa aggaagtata gtatcatcat taccagagta gatagcaaag
     4921 tcatctggaa ctaaactagc tatttctgca acctgtccta ggtctccact agcttctttt
     4981 acagcaacta tgttatcaat tttagcaagt tcaactataa ggctaggact tatatttaca
     5041 caagttcttc cagggacatt gtaaagtata attggaagtt taacactgtt agctattgtt
     5101 tcaaaatgac gcttaagtcc agctttatta gctttattgt aataaggtgt tattattaat
     5161 agagcatcta ctcctaattt ttcagcttct tgacttaagt taactgagtg catagtatca
     5221 ttagaaccac ttccagctat aacaggaact cttttattaa ctttttcaac aacatactta
     5281 atagtagcaa gttgttcttc atcagtcata gtattagctt caccagtagt accacaagat
     5341 actatagcat cagtaccatt agctatttgg tattcaacta attcacctaa tttgtcaaaa
     5401 tcaactttgt tatcctttgt aaatggtgta actagagcaa cagcagaacc tttaaatatc
     5461 atataaaatc aatcccctct tttttatttt tgtgttttat attacagtta cttctaatgc
     5521 ttgtttttga gttactatta atattttaca aaattatata tttatacaag tttgtatatt
     5581 atactaaatc gtatgataaa gccatttcag ctatttgaat tgcgttagtt gcagcacctt
     5641 ttcttatatt atcagcaaca acccaaagat ttacaccatt atcaacacta aaatctcttc
     5701 ttattcttcc aacaaacact tcatctctat catcaaattc tacagcagtt ggatattcgt
     5761 ttttagcagg attatcaact actacaacac ctggagcttc ttcaagtgtt tttactaaat
     5821 catctaactc aaatggattg ttaaactcaa catttataga ttcactatga ccatttttta
     5881 ctggaactct tacagtagtt gcagttattt ttaaatcgta gttatcaagt attttcatag
     5941 tttcatttac cattttcatt tcttctttag tgtatccatt ttcagtgaat gaatctatat
     6001 gaggaagaca gttgtaagag atttgatgag ggtagaatcc agtaggatta ccgttcattc
     6061 ctttctctaa atcttcaaca cctttaacac cagaaccaga tacagcttgg taagtagagt
     6121 aaacaactct cttaattttg tatttatcat caagagcttt aaggggaacc atagcttgta
     6181 tagtagagca gttaggatta gctattatac ccttatgatt ttttatggct tctggattaa
     6241 cctcaggaac tactaaaggc acatctggat tcattctcca ttgactagag ttatctacta
     6301 ctaagattcc atttgaagca gctattggag catacttttc actaatactt ccaccagcag
     6361 agaataatgc tatttgtatg cctctatcag taaatgaagt ttcctttaat tcttctacta
     6421 tatattcttt accacaaaac attactttag aaccagctga tttagaagaa gagaataaaa
     6481 atagattttc tattggaaaa tttctttctt ctaatacttt taaaaatgtt ctacctacca
     6541 ttccagtagc accaactatt gctacattta cttttttcat ataaattcac cccaatttta
     6601 aattattaaa acattatttt attttagtta tacaagtaag atatgttaat atttttatat
     6661 atccaaaaac tttaatatta tattaacaac tttaattata tacaaaaaac gtacaaaggt
     6721 atactttgta cgtctaattt atcactcaaa gtaaaccctg tagctcatca cctacaaaaa
     6781 taagtgacag tattacacat gttatgtgca atcccagaaa atattattgc aagaaatatt
     6841 ttcttcggct gtaaatcctt tttccaatca ttaacacctg ccagctacta ttggatactc
     6901 ttagttacag ctacctctac cctaggttaa taggatatta aaatttgatt attctaataa
     6961 taatacatat ttaagaaaaa atcaacttat ttatataaat attgctaaaa tttaaatcca
     7021 aaatttgtca attcttgttt taacacatta agattagttt cttccatttc agctagagga
     7081 agtcttaaat caccaacatt aaaacctaaa atgttcatgg cagttttaac aggaactgga
     7141 tttacttcta taaataaagc agcaattaaa gcatccatat caagttgtaa tttttttgag
     7201 ccttcaatat caccttcaaa gaatttagtt actaaatcat gtgtttcttt aggacatatg
     7261 ttagcaagta cagagataac accacttcca cctaatgata atagtggtag tatagtatca
     7321 tcattaccgg agtatatagc aaagtcttta ggtactaatt tagctatttc tgcaacttga
     7381 gctaagtctc cacttgcttc cttcacagca actatattat ctattttagc aagttcagcc
     7441 acaacagatg gctttatatt cactttagtt cttcctggta cgttgtataa aataattggc
     7501 aacttaacgc tatttgctat tgtttcaaag tgaagtttaa gacctttctc attagtctta
     7561 ttatagtaag gagttattat taaaagacca tccaccccta atttttcagc ttcttgactc
     7621 atatgaacag acaacatagt attgtttgaa ccagtacctg ctataacagg tattctctta
     7681 ttaacttttt caactgtata ttttattaca gcaaaaattt cagattcact catagtagtc
     7741 gcctcacctg ttgttccaca aacaactagt gcatctgtgc cattctcaat gtgatattcg
     7801 attaattcac ctaatttttc aaagttgaca ttattatcct ctgtaaatgg ggtaactaaa
     7861 gcaacagcag aacctttaaa taacatataa tcaaatctcc ttttttatct aaatttaatt
     7921 ttttaagtac aataataatt atagtacaaa ttaattaatt ttaataggta tagttaatat
     7981 cttaactgtt taaataagat atttatatta tattaatatt agaaaaattt taaataaatg
     8041 ttttattctc aaaatatgca tattaattta ataaaatata ccaaaatgga aataaaaaat
     8101 aaattttatg agaatttaat aactaataat taatatattt aggttataat aaatatgtgc
     8161 gtaaaatcta acaataagca ataataccgt aaatttagga ggaataatat ggatacttta
     8221 aaggaacttt ttaaaatagg aagtggacct tcaagttctc atactatggg accacaaaga
     8281 gcagcagaga gatttaagaa tgaaaaccca gatgcagaaa gttttagagc aatattatat
     8341 ggtagtttgg cagctacagg gaaaggtcac ttaactgact atataattga aaaaactata
     8401 gcacctaaaa aagtcgaaat tgtttgggaa gaagatatta taaaagattt tcacccaaat
     8461 gggatgaagt ttgaagcttt agataaagat aaaaatgtaa cagctgaatg gactgtatat
     8521 tctgtaggtg gagggactat agcagaagaa ggtcaaagaa acagtaaaag caatagtata
     8581 tatcacttag atactatgga tgagatagta aaatggtgca aagaaaataa taagactttg
     8641 gtagattttg tgttggaatg tgaaccaaaa gacataaaag attatataaa aactataaaa
     8701 gatgctatga gaaaatctat tgatgatgga ctaagtacag atgaaataat accaggcaag
     8761 ttattattaa aaagaagagc aagtacattt tataatgcat ataaaaaaga taagagcttc
     8821 tcaacacttg tatatgcata tgcactagca gcttcagagc aaaacgcttc tggtaatata
     8881 atagttacag caccaacttg tggttctgct ggagtaatac caggaatatt ctttgcaatg
     8941 caggatttct ataattatga tgacgaaaaa ataattgaag cactacttgt agctggaata
     9001 ataggtaata taataaaaac taacgcttca atatctggag cagaggtagg gtgtcaaggt
     9061 gaagttggag ccgcttgttc tatggcagca gcagcagtag catacttaaa aggtggaact
     9121 atagaccata ttgaatatgc ggcagaaata gcacttgaac atcatttagg aatgacttgt
     9181 gaccctgtat atggatatgt tcaaattcca tgtatagaaa gaaatgcaat ggcagctcaa
     9241 agagcttatg atgcagctaa ttatgcatta ttaactgatg gctcacattc tgtctcctta
     9301 gaccaagttg ttgaaacaat gaaagaaact ggtatagata tgatggataa gtacaaagaa
     9361 acagctaagg gtggacttgc taaacatttc ttctcttgct aaaaataaaa ttttccaata
     9421 ataattcctc tgttttgatt tatgaaacag gggaattatt attaatggtg aattatttaa
     9481 taatgcaaaa tatttttata ttcaatttag ctttttattt ttaattagta taacatgcta
     9541 tataatattt taattataaa gaaatataga aatatttttt aataaattag aaattggagg
     9601 agattacatg agagaggcat catatttaca agaacaactt tataaacata ggaagtcatt
     9661 aaatcctatt gcagagattg ggagaaaaga gtataagact tcaaaatata taagagatta
     9721 tcttgataaa ttaggaattg agtatgaagt ttatttagaa acaggtatag taggtgtaat
     9781 agaaggtaaa agacctaaaa aagatattgc ttttagagct gacatagatg gtcttcatag
     9841 tgaagaaggg gtaaaacatt tatgtggtca tgatggacat gctactatat tacttggatt
     9901 aattgagtat ttaaatgata ataaagaaga cttaaatgat aatatagtat ttatattcca
     9961 accagcagaa gaaggtcctg ggggagcaaa agctttaata gatgaaggag tgcttagaaa
    10021 atacaatatt gatgaaatat atgggcttca tatatatcca gagttaccag aaggttatgt
    10081 aggtacaaga gagggctatt ttatggctca aataggagat attgacatag aaatagtgtc
    10141 taaaagtgga catggggcta tgccacaaaa tggtattgat ggtatagtga tagcttcaaa
    10201 ctttgtaagc tcacttcaaa ctatagtgtc tagaaatata agcccaatag acaatgcagt
    10261 gttgactatt ggtagaattg aagggggagc tagaagaaat ataatagctg aaaatataaa
    10321 gatggaaggt actatgagat gtttcagccc agaagtgtat gagaaaatga agcttagagt
    10381 tagagaactt gctagagggt ttgaattggc ttataactgt aaggttaata taaatattat
    10441 agatgattat attgctgtta aaaatgacaa ggatttgtat caagagtttg tagaggctat
    10501 tggaagtgat actgttgtag agttagagcc acttatgata tcagaagatt tttcttatta
    10561 tcaaaaagaa gttccaggat tattttttat gttgggttca agaaacgaag aaaaaggatt
    10621 tgtaaatggt cttcataata taaactttaa ttttgatgaa aagatatgtg taaatgcatt
    10681 gaatgtttat tcaaaattat taaaatacaa agaagcaata gattaggaag ataagttata
    10741 aagattaaaa agcataaata cctaaattta aaaaaagtgt attaataaat accccttaca
    10801 tatttgtata aatttatttt aattgacaaa aatacaaata agaaggggtg atttttttga
    10861 aagaaaaagt acaaagagaa aagaagataa ctaatggcaa gaatactaaa atattgacaa
    10921 atgatgatat aatgaaatat gagatagcct cagaactagg tcttatggat aaagttggtg
    10981 aacgtggatg ggcaggactt acagctaagg aagcaggaag aattggaggt atgcttacat
    11041 cacgcaaaaa gaggcttaaa aagattgtgg aggagcagaa taaaaaggat gagtcaatat
    11101 agtgattttg cgtttgtata tgatgaactt atgaatgaag ttgattataa tggttgggtt
    11161 aaatatatag aagatataat agatagtgaa ggtgtaaaag taaaaaatat attggaattg
    11221 gcatgtggca cgggtaatct tacaattcca ttgactaaaa aaaattacga tatagctggt
    11281 atagatatat ctgatgagat gcttagtgta gcaagagaaa aggcagaaaa agaaggagta
    11341 gaattagttt tacttcaaca agatatatca gagcttgatt ttgaaatatc tgatttggac
    11401 tgtgtacttt gtgcttgtga tggatttaat tatataactt atgatgatga tttggaaaat
    11461 gtattttcaa agacatatga attattaaag aaagatggaa tattcatatt tgatataagt
    11521 tctttttata aattagccaa catattggga aataacatgt atggagaaaa tagagaagat
    11581 atagcgtata tgtggcaaaa ttactttgat gaagaagaga atctaattga aatggaactt
    11641 acattcttta ttaaagatga atgtggaaag tttgaaagat ttgaagagat acaccaacaa
    11701 agagcatata ctgaagatga gatagttgat atgcttgaaa aatctggatt taaggatata
    11761 aaatattatg gggattttac atttgacaaa cctagtgaag aaagtcaaag aatcttcttt
    11821 gtatgtaaaa aataagatat tcttgactat ataagataat tattaaaaat aaccatgata
    11881 taaaaatata taaaatgcag ttttatagat taaaagtaaa gtgatgatat tttatgattt
    11941 ttattttcta ataattataa agttttatag ataaaaatta aaatataata actattgctt
    12001 agtaaaatta aaataaaatg ggttttgccc aaggttaaga tagataagta tttatagatt
    12061 aaaattaagg tagtaatata ataaaaataa gacaattaac gggacatgga ggaatattaa
    12121 tgagagatta tgtactaaga gcgacatcag gaaatggtca agttagagca tttgtcgcaa
    12181 ctactagaaa tacagtagaa gaagctagaa gattacatga aactacaaaa gtagcaacag
    12241 cagcacttgg aagaacatta acagccacat ctataatggg attaatgatg aaaaatgata
    12301 gtgataaatt gactgtaata ataaaaggtg gaggtccaat tggaactatt atcgccacat
    12361 cagactctaa gggaatggta aaaggatatg taggaaatcc acaagtagag gttgaagact
    12421 atccaaatgg aaagttaaat gtagcagcag cagtaggtac agaaggtgtt gtaaaagtta
    12481 taaaagactt aggtcttaga gaaccatata atgggactta tccattggta agtggagaaa
    12541 ttgcagaaga ctttacttat tattttgctg tatcagagca gactccatca gtagtagctt
    12601 taggtgttct tacaaaagaa gatgaagtag agtttgctgg aggatttata gttcaattaa
    12661 tgccagatgc agaagaagag actattgcta agttagagga aaatgtagct aaattacctt
    12721 ctataactaa catgttaaaa gaaggaaaat caccagagga tatattaaat attgttttgg
    12781 atggtttgga acctaaaata cttgatacct gtgaagttgg gtttatgtgt gaatgttcaa
    12841 aagaaagagt aaaaactgct ttggttgcaa ttggtaaaaa atcattggct caaataatag
    12901 aagaagataa aaaagcagaa gttggttgcc agttctgtaa taagaagtat atgtatagtg
    12961 aagaagagtt gcttgaaatt ttaaaggaaa tgtagactat aaaaatagtt gtattgatat
    13021 aagtaatgtt atagtaatat agtaaagtcg tattgatata gtaattaaag tataatttat
    13081 taataataga catatttata ttaaaaaata agtttaataa atttttaagc ttaataaatt
    13141 ttaagaatta agaagctttc tggctgtaat gtttgcaatg ttgcagttag aaggcttttt
    13201 tattttatta tagttcgtgt caaagattgt atgaaacaat aaaatattgt aaaataattt
    13261 gtatatttag acctgattga cacaaaggtt tataagatat ttgagttgat tatacgaaat
    13321 tctatgaagt agtaaccctg tgactttaat catgggaggt tcagaagaag gagaaaattt
    13381 taaatatgga aagtaaaacg aagtacatag gaataagtat attaatatta ttgatgatat
    13441 ttttaattgc aaagaatttt aacggaaaac ttgaaatacc tattcctgag agtaatagta
    13501 gaaaatttaa atcagcagca gtgttttatc ctcaacatca agatgatgag gttttatggg
    13561 gaggaagtgc cattgtagat gctataaaac agtgtggtgt tgataatgta tatgtggtat
    13621 tagtatcaga tggttctgga gttaacgttt tcaaagctaa cacaaaattt agaaatttaa
    13681 ctaggaagca aaaggaagaa cttagaaata acgagtttaa atcagctttg agagagcttg
    13741 gagtgaaacc tcaaaatgtg attatacttg cagatataga taaaaaggaa ggcacacatt
    13801 atgaattaat ggaaaagact atattggatt ttgaacataa gtttaaaggc aatgtgaccc
    13861 atatagccca tcactataag tatgatgacc atataatgca tagaaagaat ggggaagttt
    13921 taaagaaact taaacgtgaa ggtaagataa aagatgactt gtattttgtt aagccaaaat
    13981 atgtaaagta tataccaaga aatgaaagag ctatctataa agcaatggat tttgatgact
    14041 acaataaggt taagagtgct tgttatgaat ataaggttgt aaattcaaag cgtaaaatgt
    14101 atggtatagg atatatttcg gcacatagtt attttgaaca tcttctaaat gaccctgaac
    14161 ttacgtctgt attaagtgaa gattaatttc ttataagtag taagttatgt ttgataagtt
    14221 gattggaatt ttctatagtt ttgaaggtat tggaaattta gttttattta aagatttaaa
    14281 gatagaggtt tttagtgttt aaaattttaa agttaagttg aataattgta tagaattatt
    14341 tgtgaaagaa gaaacagtgt aaaacatagt atcttattta tggttttaaa atcataggca
    14401 agatactttt tttatgtaac taataccatt ttgagattat taacttaaga attttatata
    14461 atttaacact catatataat gatgatattt aggtagaata gatgtaatta cgcttaaata
    14521 aaataactgt attttttata aaaatccaaa ccctacacta tactaccaga taaaatctac
    14581 atgctataat ttgacctgtg tttataaaaa aaggagtaat ctaataaaat agtatttcac
    14641 ttatgtataa ctaacaacta ttattacgat tttctctaaa taatttaaag aaaaataatg
    14701 caggaggtaa aatatgaaca atgaatttat aattttaaaa ggttgcaaag agaacaattt
    14761 gaacaatatt tccttaaaaa taccaaaaag aaaaataacc atattcacag gggtgtcggg
    14821 ttctggaaag tcatctattg tatttgagac aattgcaaaa gaatcacaac gccaactaaa
    14881 tgaaaggttt agtacatttg taagaagttt tttacctaag tatggagagg ttaaagctga
    14941 ttgtatagaa aatttgtcaa caccaattat catagaccaa agtagacttg gtggaaattc
    15001 aaggtcaact ctaggtacta ttacagatgt aaattccttt ttaagagctt tatattcaag
    15061 gtttggtagt gagtatatag gtaatgccaa tatgttttct tttaataata ttgatggtat
    15121 gtgcccacac tgtcatgggc ttggaaaaaa gctagttcct aatatgaacc aaattcttga
    15181 tatgaataaa tcattaaatg aaggtgctat attgctttca gggtttggag tgggttcttg
    15241 gcattggaat atattcgctg aatcaggatt ttttgatatt aataagagga tatgtgatta
    15301 ttcagaagta gaattggaaa agtttttgca tggtaaagca gaaaagatta agatagaaaa
    15361 tgctggtcaa actaacatga catatgaggg attaatggtt aaatttaatc gcctatactt
    15421 aagtagagag ggagaaatat ctgaagctac taagaaaaaa ttgagtaaac ttcttataga
    15481 agatagatgt cctatatgtg atggaagaag acttaatgaa agagtatata ggtctttaat
    15541 taatggttat aatatagcag atttaactag tatgcaaatt gatgaattgg ctgaagttat
    15601 taaaagtata gatgagccag aagcacaacc tttaattaaa ggaataatag aaaagttaaa
    15661 cagtatcata gatataggtc ttggatattt aacactagat agagagacat cttctctatc
    15721 aggaggggaa tctcagcgta taaagatggt taagtattta aatagtaatc ttgttgattt
    15781 aatgtatatt tttgatgaac caagtgtagg acttcatcca aaagatgtgt ataaattaaa
    15841 taatctttta aaaaaattgc gtgataaagg gaatacaatt atagttgttg aacatgaccc
    15901 agatgttatt aagattgcag accatattgt agatgtagga ccaaaggcag gtaagtatgg
    15961 tggagaagtt gtgtatgaag gcagttatga aaatctactt acaagtggta ctttgacagg
    16021 gaatgcttta agtaaatttc ttcctattaa agaaagtgta agagaacata gtggatattt
    16081 agaagttaaa aattgtaata aaaacaattt aaaaaatgta tctattaaga tacctaaagg
    16141 tgtattgact ttagttactg gagtagcagg agcaggtaaa agtacattga ttaaggatga
    16201 atttttaaag caaaatccta gtgctgtact tatagaccaa agccctgttt cagcaaattc
    16261 aaggtcgagt ttggcaacat atagtggaat tatgaacaac ataagaagca tattttcaaa
    16321 aacaaatggc gtaaatgctt ctttattcag ttcaaattca gaaggagctt gtgataactg
    16381 taagggttct ggaatagtag aaatgaatct tgcttttatg gagagtataa agagtacctg
    16441 caatatttgt gaaggtaaaa aatttaaaaa agaagtttta gaatataggt ttcaagggaa
    16501 gaatataatt gaagttcttg aaatgtctgt tttagaggca attgagtttt ttaatttaaa
    16561 acagattaaa acgaaattac aatgtattga aaaaatgggt attggatacc ttactttagg
    16621 acaaactctt gatactttgt ctggaggaga gtgtcaaaga ttaaagcttg ctagtgaatt
    16681 aaataaggaa agttcagtgt atatattaga tgaaccaaca acaggtcttc atatggctga
    16741 tattgagaat tttataagta taattgaaga gattgtagat aatggaaata cggtcataat
    16801 aattgaacac aatatagatg taattaaaag agctgattgg attattgagc tgggacctga
    16861 aggaggaaca aaaggcggga gagtaatatt tgaaggtatt ccaaaacaat tatgtaacaa
    16921 taaaatatca ttaacagcaa aatatatagt ataataaata aaatataata aaattataat
    16981 gatattttaa taaatataat gggtatataa aatgataatt aaacttaaca gtaataaata
    17041 atgtgagaaa tattcaaatt taattaaata aagtgtcaga aatttgttta aattttagta
    17101 ttttaacggt tctgaccttt tttcttattt ttatggagaa tatcgaggat ggagtctcta
    17161 atagttactt ttttgtgtct acttgcaagt ttgattaaaa aatggctata cacacttgtg
    17221 acttcagtca tgggaggttc agattataat cttaatatat aatttgtgaa ataatttgat
    17281 gacgatttgc tgatgtataa acaataaaga ttaactacat taaaatttaa atcttgttaa
    17341 gtaaaactta taaaaggaga gagatggatg ataaatgata ttctaacctt attatctgaa
    17401 agaagtgatt tttttataaa gctatccata gaacatttga tgatttctat tatttctata
    17461 ataattgcga ttgttttggg tggtataatt ggtattattg taagtgaatt tcataattct
    17521 tctaaaccta tattgggaac tatcaatttt ctatacacca ttccatctat ttctatgcta
    17581 ggatttctga taccattttc cggaattgga aatactactg ctgttattgc acttaccatt
    17641 tatgcattat tgccaatggt tagaaataca tatactggaa ttactaatgt ggataaaaat
    17701 attcttgaag cagctagggg aatgggaagt acagattttc agatattgta taaaataaaa
    17761 cttccgttag ccctacctgt tattatgtct ggtattagaa atatggtgat tatgacaatt
    17821 gcattagctg gtatcgcttc atttataggt gctggaggcc taggtgtagc catatataga
    17881 ggaatcacga caaataacac aataatgaca attactggaa gtatacttat tgctctatta
    17941 gctttaatat ttgattttat tttagggctg gtagaaaaat tttttcaacg aaaaagaaaa
    18001 aagaaaaata ggaaaaaagt ttttgccatt ctaggaagcc ttattcttat agtaattatc
    18061 tttacgtctt atttatttaa tccttcaaag actcaaacta ttcatattgc tactaaacct
    18121 atgactgaac aatatattct tggtgagatg ttggatattt taattgaaaa aaacacaggt
    18181 ctaaacgtgg aaattacaca aggtgtagga gggggaacat ctaatatcca gccagctatg
    18241 gagaatggag aatttgacat ttatccagag tatacaggta caggctggaa tatggtactt
    18301 aagaaaaatg aattatatac tgaaagtatg tttagtaaac tacagtcgga atacaaaact
    18361 aaacttaaaa tgcaatggac tggtatgtat ggtttttcta atagctatgc attagcagtt
    18421 agaaaagaag ttgcagataa atacaattta aaaaatataa cggacttaaa agatgtatct
    18481 tctaaattga catttggagc tgaatatgac ttttatgaga gagaggatgg atatactcca
    18541 ttgtgtaata catatggact gaactttaaa gatacagttg atttagatat tggattaaaa
    18601 taccaagcta tcaatgaagg taagattgac gtaatgccta tatttacaac tgatggtcag
    18661 tatagtgttt cagatgttgt agttctaaaa gatgataaaa atttctaccc atcttatcaa
    18721 tgtggaaatg ttgttcgttt ggaagtatta gaggaaaatc cagaactatc aaaggtattt
    18781 aagatggttg gagatatttt gactgataaa gaaatggcta aaatgaatta tgacgtagaa
    18841 aacaacaatt tagaacctag agaagttgct cgtgagtttc tgaataagaa aggtctttta
    18901 taaattggag gaaatataat gacacctatt atacaattta aaaatattaa aaaacaatat
    18961 aatgataaaa ctataataga taatcttaat ttagatatag aaaaaggaga gtttttgact
    19021 gtaattggtt cgtctggttc tggtaagaca acactcttaa aaatgattaa tggtctgatt
    19081 ttgccagatg gaggaaatat tttgataaac caaacagata tcaaaaacga ggacttaatt
    19141 aaattaagaa gaagaatagg atattgtgta cagggaagtg ttctttttcc tcatatgact
    19201 gttgaagaaa atatttcata tgtaccaaat ctcctaagta aaaagaataa attagaagta
    19261 aaaagtgctg taaacaagtg gatggaaata gtgggattgc caaatgatat gaaagtgcgt
    19321 tacccatcag aattgtcagg tggtcagcaa caacgtgtag ggattgcaag agctttagct
    19381 tcttctccag aaattttatt aatggatgaa ccttttggtg cagttgatga gattacacga
    19441 aagcaattac aaaaagagat aaaagaaatt cataaaaaaa caggagttac gattatcttt
    19501 attacgcatg atatttatga agctttgatt ttgggaacaa agactttggt attaaatcat
    19561 ggagttgttc aacagtatga tactcctgag aatattttaa atactcctgc taaccaattt
    19621 gtagaccagt tattgaatat aagaaaatct ataatagatg atgataattt aaaagaataa
    19681 atttaaattt aaaatcaaat tgttgtgata aaaactattg atttagagct aacttcaagt
    19741 tgtatacttg aagtggaaaa gttagattaa ggtatagaag ggcatttagc agttggctgt
    19801 agacgaggta actgctgaag gtgctttcaa taaatggcta gaacctgtat ctgttgaaga
    19861 ttataataaa ttgaaataag aagctcaata tacagagtag ataggcactt caaatagaag
    19921 tgtcttttgt gtgccataaa ataaatcaaa atattagcaa agaatgtgaa aaacaaaata
    19981 aaataatatg ctatctttaa aataggaggg atgctatatg gataacaatt tgattaccat
    20041 tgaaaaaact ttagattata tagaaaaaca tcttttagat gagatgttag atttaaatgt
    20101 aatttctaaa gagataggct attcaaaata ccatttacat agaatgttta catcagttgt
    20161 cggatttagt gtacataatt atatacaaag acgtagactt actgaagcag cgagaatgct
    20221 aatttttaca gaaaaaccaa ttattgagat tgctttaatc tcggggtatg atacacaacg
    20281 ctctttttca agagggttta aaaaaatgtt taaacgtagt cctaattttt ttagaagaca
    20341 tcaagatttt ttaccactac aattaaaatt tgatattcat aatagaaaaa aattacgtgg
    20401 agatatggtt ttagatgtta aaaaagttaa ttgtgcagat ataaatattg ttggctattc
    20461 tggtaaagta agaaaaggat ttcatattat tggaaaatgc tggagggatt tgcataaaaa
    20521 taaagagatg atagaaaata aggtagattt aggtttttta attggagtta gtgattatag
    20581 caattttaaa gagattgaga atactcctac atttacatac attgcaggtg ctcaagtagc
    20641 tacttttggc aaattaccta atggaatgga aaaattcgta ttaccgaaaa gtaattatat
    20701 tgtttttact tttattggga aaaatgagga tagtatgcaa aatgttgtag aatatatcta
    20761 tcaagaatgg ttccctaatt caagttgcca gtttaatgaa aataatttat atgactttgt
    20821 aaaatatgga gaaaaaactg atgagaatgg tttgagtgaa attcagtttt gggttccaat
    20881 catttaaaag aaggtattat gtataaagta gatggattta tataggcaaa gttttacagc
    20941 agtatgtatt tataaataat ttatagtttg taggaaagaa ataattttac tataagatta
    21001 tgataaaata cacaatacta tgacaaaata gtgttaaata atagatttaa ttttgcatag
    21061 gaaaagttct ataatggtgt gtattcataa ataatttata atctgtagga aagaaatagt
    21121 tttactataa gattatgata aaatacacaa tactatgata aaatagtact aaataataga
    21181 tttaattttg caaaggataa ggtgttttaa taaaaatgaa tgtagaaaac gaattactta
    21241 aaaacctaga tagattgcat acaacagagt taggcgttgt aagaattaag aaaaatcttt
    21301 ctttggagac aaatgatgta gtgaactggt gtaaaactaa aatagaatct ccaaatgcca
    21361 ttattaatag aaagggaaaa aactggtata taagtgtata tgattgtatt ataacagtaa
    21421 acgctcacag ttatacaatt attacagctc ataaagaaaa gaaatgaatt gcaaaataca
    21481 aaggtcaaaa gcattttcca ctgtttgtag tacctatgaa gaagtatata aaataatatt
    21541 acttttgaat aataatttaa attttggagg ttgaaaaaat gaatgatact gtaaaagaaa
    21601 aattggcaga gtcactaact tctgatacta ctgatatttt accttatttg ccttatttat
    21661 tacaagattt ttgggaatta ggaagttcac cagaacaaat gttacaatta tttagaaagc
    21721 atataccttt taatgaggat acaaaagttt tggatttagc ttgtggaaaa ggtgctgtat
    21781 ctgtgaagct tgcaaaagag ttgaatatac atatcaaagg tattgatttg atacctgaat
    21841 ttattaatta tgctagaaga aaggctattg aatataaggt tgatgctttg tgtgattttg
    21901 aaatgaatga tgctaatagt tctgtaaaaa ttgagcatga ttatgattgt gtgatttttg
    21961 gagctgtagg ggatatttta ggtaattata aagaaactat aagtaagcta ttacctacaa
    22021 taaaaagtaa ggggttcatt ttaattgatg atgcatatgt agtagataaa tctacaaatg
    22081 aacagcttaa ttttgagagg gtgtatccca cttacaatga gtggtgtata ttgttcaaag
    22141 aattaggtct gaagctaatt gaatgtatac catgtttaga acttgaaaat gatgttgaag
    22201 aggaaatgaa ttggattata actcgtgcaa atgagctaat taaaaaatat ccttctcaaa
    22261 aagatatgtt tgaacaatat gttattagcc aaaaaaatga atatactgat ttacagcagg
    22321 accttgttgg agcaacttgg attttacaaa aagaataaga taaatattag actagataat
    22381 caatgttata gtgaattatc ccatgtctag tgaatataaa taacataaaa aaccgtcaag
    22441 atgaatagtt tattcgtgct tgacggtttt tagtatttgt tgaagtaaaa ttttatttct
    22501 ttagtatatc acttttagtc aatccatttg atttagcttg agtattccat tgatgattcc
    22561 ccataagttt atataggaat ctagggaagt taggtgaaat aaaaatagta cttttgttgg
    22621 aatcatctat atgtatatta tcactaagta tattttttga aagttctttt aatgcaattc
    22681 ctaaatcttt tttaggtcct ttacccaaag gtacggattc tagtgaacca aacatctccc
    22741 cagctccaat tccaatacct tgaacccatg taaagttgat ttttttacac caatgctcta
    22801 tcatttgaat tgccaaatga ttatgttcac cttcattgaa tccacagtta actattgcat
    22861 acactggaat aggtttttta ttttgtgttt ttaaaaattt ttcacatttc tctaaaaaat
    22921 gaagtaaatg agatggaata ccatctacat aaagaggaga tgcgattatt aatctatcac
    22981 aattaagtat ggcattaaga gcgtcaggat acttagatgt gctatctact ttaaatgtaa
    23041 aaactttatt atcattttga ataagttgtt ttaattcaga taaaaaatac tgtgaattat
    23101 ttttatttac ttttggacta ccatttatca ttactatatt catatggatt cacctcttat
    23161 atcttgtata ttttcataga aacaaacaga gtgttctttt atattgaaat ttatagcatt
    23221 tccatttact aatttcgtag cagtttcttt ttcagcttct gtaatacaat ctccatagaa
    23281 tatagctttt aaatttatct gtttatcata tctgctttta tgacgcatca tattatcttt
    23341 tattacaaag tatggaagaa tataattaat gcttttatca agtacgtttt tgacaaaagg
    23401 gctaaaccct ccgtaacagc atctgcttat tataataatt ccatcagagt catttataag
    23461 tttacccatg ttgctgtaat aatcttttac aacacattca gaaggagttt taatccagca
    23521 accaaaacaa ccaatacatt tgtggatttc ttttgtgttt ggaagtattt ttatattgtc
    23581 atcaagtttg ggtaataact tttctaattc tttgtcagtt aaatcatgta ttaaaagcct
    23641 cataaagtcc atctcctttt attatgtatg tttacttcta aaaattatat cttatagatt
    23701 tttcagtaaa tctttacacc aattaataac aaaattataa tagtcaactc cataaatcat
    23761 tgttgcatac atatatgtat attggtcaac gcaatgattt tcttctgcat catttttttg
    23821 tttacttaat ttatttagaa gtaattctac ttcagttatt aacattttta agtttgtttc
    23881 aattatttct ttgggtagat acttgtaaaa gaatatatta acaaggtggt cttgctttgt
    23941 tttagaaaaa tttataggtt tctttaacca ttcaagaaat acttctttac cagtattagt
    24001 tatggaatat aattttttta atttgctacc atctataact tcgtgatagt gtatatatcc
    24061 ttttgtttct aatcttttaa gtgcaggata gatacttcca aagctggcgt caaaaaagta
    24121 cgaggtactt tctgacatta tttgttttaa gtcgtatcca cttaattctt tttccattaa
    24181 aaatcctaaa ataatgtatt ctaacatatt acctcctggt atcaattaga tatatatcga
    24241 ttcgatatat ttattattac agataataaa tcttacgtca agatgctttt taagataagc
    24301 taaaaagaat taggagttat tgataaatat gcaaaaaaat taatataaaa ggtaattaaa
    24361 tatcaatgaa gatttaaaaa tagttaaatc aaagtaagag tctaatatat aaaatttata
    24421 gtaactttat ggtttaaata taaacttgcg tgctattgac aaagtataaa attagcagta
    24481 aaataattgc atgagcaaat agattattgc gaacgcaaaa aaataaggac aatagatatt
    24541 aaaggagaga atatgagaga gctattaaaa tgggtttcca ttataaatag atacaataca
    24601 aattatattg acaggaaatt ggcggatatt ggaatcaata atagccaata tttttatgta
    24661 ttacacatat gtaataatcc aggtattaca caggacacta tatttgagaa aattcttgtg
    24721 aatcctagta atataacaag agctttggca aatttagaaa aagaaggctt tataacaaga
    24781 gaaccaggtg taaaggataa aagaacatgg catttatatc ctacagaaaa atctttagga
    24841 tgttatgatg aaatactaaa aataacaaat gggtatgttg aggagttgtt gagttcattt
    24901 gagaaagaag agcaagagtt gtttatgtca atgttaaaga aagttgcatt tttggctatt
    24961 gatatgaatg aagaagcaaa attggaggcg aaggaaaatg gaagaaaaat ataaggagcc
    25021 attggaatat gaaaaggttt caaagttatt aactaagtac gcaattccta gtattatagc
    25081 aatgttagta agtgctttat ataacattgt tgaccaaata tttattgggc aaggtgttgg
    25141 tgtattgggt aatgctgcta caaatgtcac ttttccacta acaacaatat gtactgcaat
    25201 tgcattattg ttaggaattg gaggagcgtc taagtgttca ttggaattag gtgctaaaaa
    25261 ttcagaaaaa gctacaaaag cagcaggaaa ttctatatgc ttgatggctg tttttggaat
    25321 aagtttattt atagtagtat caatattttt aacaccacta ttaaaattct ttggctctac
    25381 atcagaaata ctaccttatg cacacacata tacaaaaatt acatcaattg gattaccatt
    25441 tcttataatg tccactggaa tgagtaagtt gatacttgca gatgggagac caaaagcttc
    25501 tatgttttgt atgttaatag gagcagttat taatacaatt ttgaatccat tattcatttt
    25561 tgtttttaat atgggaattg ctgggtctgc tttagcaact gttattgggc aacttatatc
    25621 ttttggaata agtgttcatt atataaagaa ttttcaacat attaaattac ataagaaaag
    25681 ttttaaatta gatagtataa tttataaaca tatatttgcg ctaggttcaa gctcatgttt
    25741 taatcaatta gcaatgactg taacacagat agttatgaat aatacattgg catattatgg
    25801 ggctaggtca gtttatggaa gtgagattcc acttgcttgt gtcggtatca ttattaaagt
    25861 aaatatgatt tttatatcta taattattgg tatatcacaa ggtatgcaac caattatagg
    25921 gtataattat ggggctgaaa agtataatcg tgtaaaagaa gcatataagt ttgcagtggg
    25981 aagtgcaact attatatctg ttattgcatt cttatgtttc caattcttcc caagacaaat
    26041 tacgtctata tttggtaacg gaaatgagac ttattttata tttgcagaac attattttag
    26101 aactttcttg ttcttaacat ttataaatgg tgttcaaata gtatcatcaa ctttctttac
    26161 atcaattggg aaatcagtgt tgggactttt tacttctttg actcgtcaaa ttttattttt
    26221 aattccattg atagtaattt taccaatgtt tatgggaatt gatggagtaa tgtttgcagg
    26281 accaatagca gatgcagcag cggcaatggt atgtatatac tttgcagttc atgaaatgaa
    26341 atcattgact ataaagcaaa ataatttgat gagtaaccat aataagattg cagaggtata
    26401 agacaatatt taagaagtat agaaaattaa tttaaaaaat ataataaatt aatttaggaa
    26461 gtgtagaaaa ttaatctaaa gaagtataat aaactaattt aggaagtata gaaaattaat
    26521 ctaaagaagt gtaataaact aatttaggaa gtatagaaaa ttaatttaaa gaagtataat
    26581 aaactaattt aagaaatata gaaaattaat ttaaagaaat acaatacatt aatttttaag
    26641 taacattaaa ttaatgtaag taaaataatg ttcatatgaa tatttaaagg tgcattttct
    26701 gaagctaagt atatgttgta gagtgttatg tctctctatc ctgtgaagtt taatagctca
    26761 agaaaatgca ctatttatga tgattatttt gttttatttc caattttgga aataactatt
    26821 ctaattaaat ctaaaattat accataaata agaaaagaaa gtatagatgc tatgtaccaa
    26881 taaatcgaaa ttgaccctgt aattgttcta catgggattt gcatacctga atccataaaa
    26941 acaaaaaaac tatcaactct ggaaataata gacaaaatag gatttaaacc tattagaaca
    27001 atactttttg agtcttgacc aatttgatgc atataaatct caaataatgc aattaaaaaa
    27061 aaccaagatg taaaagattt aataattttt ttcatcaaaa tacctcacat aatataaatt
    27121 ctgtttgcac tatttgctta ctacaactat ttatctccat tcagtcacat cacttaattg
    27181 taatctaggt cttcttgatg gttcttcatc aggataacct aaagatattg ctgccactaa
    27241 ttgactatct gtatttaacc attcacataa ttcacgatat gcaaagtata catcacaaat
    27301 ccaaagacta cctagaccta attccacagc tgtaagcgac atattctgaa ttgcagctcc
    27361 aattgattgc atatttgcca tttcatagaa tcgttcttca ggggatagct tttctaaagg
    27421 tgatttacct aatatattta aaataaaaat tgttactgga gcttgtttca tgatttcaac
    27481 tgtgtaattt gcaccagcga tatgttgacg acttcctgga agaagcccat tatcattaat
    27541 ttcattttga atacctttgc tcatggcttt aagcatagat tctttttcct tttcagtgat
    27601 gacaacaaaa cgccatggtt gcctgttttt agatgaaggt gcatttattc cagcttcaat
    27661 tattttctct atagtttcat gtgaaatgct ttgattttta tattttctga tacttcgtct
    27721 ttttgatata gaatcactta tcattttaag taacctccgt ttattttttg gaaaaatcta
    27781 tttaattttt attttctatt ttaattattt tagcattaat cagaaaaaaa gtcattgatt
    27841 ttagtataca attattaatg attatattaa gaattgtatc aaaaagtatg tgattatact
    27901 aagggattat attaaa
//
LOCUS       DAYASV010000040        27657 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYASV010000040 DAYASV010000000
VERSION     DAYASV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.040"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            283..627
                     /locus_tag="ACGI68_001705"
     CDS             283..627
                     /locus_tag="ACGI68_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFH0138147.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            658..2259
                     /locus_tag="ACGI68_001706"
     CDS             658..2259
                     /locus_tag="ACGI68_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFH0138148.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFSKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2278..3378
                     /gene="dprA"
                     /locus_tag="ACGI68_001707"
     CDS             2278..3378
                     /gene="dprA"
                     /locus_tag="ACGI68_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFH0138149.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3411..5498
                     /gene="topA"
                     /locus_tag="ACGI68_001708"
     CDS             3411..5498
                     /gene="topA"
                     /locus_tag="ACGI68_001708"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFH0138150.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNIGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5663..6448
                     /gene="codY"
                     /locus_tag="ACGI68_001709"
     CDS             5663..6448
                     /gene="codY"
                     /locus_tag="ACGI68_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFH0138151.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6555..7820
                     /locus_tag="ACGI68_001710"
     CDS             6555..7820
                     /locus_tag="ACGI68_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFH0138152.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNDAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7851..8621
                     /locus_tag="ACGI68_001711"
     CDS             7851..8621
                     /locus_tag="ACGI68_001711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0138153.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8730..9176
                     /locus_tag="ACGI68_001712"
     CDS             8730..9176
                     /locus_tag="ACGI68_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFH0138154.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9194..10387
                     /gene="nifS"
                     /locus_tag="ACGI68_001713"
     CDS             9194..10387
                     /gene="nifS"
                     /locus_tag="ACGI68_001713"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HFH0138155.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10441..10881
                     /gene="nifU"
                     /locus_tag="ACGI68_001714"
     CDS             10441..10881
                     /gene="nifU"
                     /locus_tag="ACGI68_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HFH0138156.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11170..12249
                     /gene="mnmA"
                     /locus_tag="ACGI68_001715"
     CDS             11170..12249
                     /gene="mnmA"
                     /locus_tag="ACGI68_001715"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFH0138157.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12392..12630
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12698..15337
                     /gene="alaS"
                     /locus_tag="ACGI68_001716"
     CDS             12698..15337
                     /gene="alaS"
                     /locus_tag="ACGI68_001716"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFH0138158.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15362..15619
                     /locus_tag="ACGI68_001717"
     CDS             15362..15619
                     /locus_tag="ACGI68_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFH0138159.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15629..17800
                     /locus_tag="ACGI68_001718"
     CDS             15629..17800
                     /locus_tag="ACGI68_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138160.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDIMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18006..18431
                     /gene="ruvX"
                     /locus_tag="ACGI68_001719"
     CDS             18006..18431
                     /gene="ruvX"
                     /locus_tag="ACGI68_001719"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFH0138161.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18509..18796
                     /locus_tag="ACGI68_001720"
     CDS             18509..18796
                     /locus_tag="ACGI68_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFH0138162.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19078..19536
                     /locus_tag="ACGI68_001721"
     CDS             19078..19536
                     /locus_tag="ACGI68_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFH0138163.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19653..20390)
                     /locus_tag="ACGI68_001722"
     CDS             complement(19653..20390)
                     /locus_tag="ACGI68_001722"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0138164.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20607..22283
                     /locus_tag="ACGI68_001723"
     CDS             20607..22283
                     /locus_tag="ACGI68_001723"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFH0138165.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22489..22689
                     /locus_tag="ACGI68_001724"
     CDS             22489..22689
                     /locus_tag="ACGI68_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1290 family small acid-soluble spore protein"
                     /protein_id="HFH0138166.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22743..23906
                     /locus_tag="ACGI68_001725"
     CDS             22743..23906
                     /locus_tag="ACGI68_001725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0138167.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24117..24539)
                     /locus_tag="ACGI68_001726"
     CDS             complement(24117..24539)
                     /locus_tag="ACGI68_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HFH0138168.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24560..25396)
                     /locus_tag="ACGI68_001727"
     CDS             complement(24560..25396)
                     /locus_tag="ACGI68_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFH0138169.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25694..26290
                     /locus_tag="ACGI68_001728"
     CDS             25694..26290
                     /locus_tag="ACGI68_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFH0138170.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26320..27039
                     /locus_tag="ACGI68_001729"
     CDS             26320..27039
                     /locus_tag="ACGI68_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFH0138171.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27041..27580
                     /gene="scpB"
                     /locus_tag="ACGI68_001730"
     CDS             27041..27580
                     /gene="scpB"
                     /locus_tag="ACGI68_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFH0138172.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11204 a         2892 c         4993 g         8568 t
ORIGIN      
        1 gaccggatac aatgtatttt caacattttt caaatagctt acaactaata taaataaaga
       61 taaatatcgt ataaaagcaa ctaatttttc tctgttcatt aaaggcactc ctccaaaaaa
      121 ataaactttt cctatgaaaa ctagaataat atcataaaag aataaagtca atagaagtat
      181 ttattaatat tgatggaaat aaaatcaatc caaaaacttg aatagtaaaa ttgtatactg
      241 accataattt aaaagtgaaa atttaatgta ttggagtgaa taatgaataa taaagaaaaa
      301 ggtgattttg gagaaaaagt agcagttaat tatttgctat caaaaggagc aaagatacta
      361 gaaaaaaatt acagattaaa gataggggaa atagatataa tagcaaaaat ggaagatgaa
      421 attatatttg tggaggtgaa atcaaggtct aacataaagt ttggctaccc ttgtgaatct
      481 gtaagcttca aaaaaagaaa aaagataatt ggagttgcaa attattatat tataaaaaat
      541 aacttaaata acactcccat aagatttgat gttatagagg tatatttatt agaaaaaaga
      601 ataaatcaca taatgaatgc tttttaaaaa agataatgtt tttggaaagg attaaatatg
      661 ttaagtatta ttaattcaag caacttagta ggaatcgaca gctttttagt taaggttgaa
      721 gtagatgtaa gcaatggaat acctagtttt aatattgttg gtcttccagg caaagaaatt
      781 aaagaagcaa gagaaagagt aaagtcagca attcttaata gtggatataa atttccaagc
      841 acgagaatag ttgtaaattt atcaccagca gatataaaaa aagaaggagc ttttttagat
      901 ttatctataa gtataggcct tttaagagag ttaataaaaa aagatgaaaa ttacataaga
      961 gaaagtatgt ttataggaga attatcatta gatgggaaaa taagaaaagt aagaggtata
     1021 ttgcccataa ttatgggtgc aaaaactcag aacataaaac gtatttttat accaattgag
     1081 aacataaaag aaagtctatt tgtagatgaa attgatataa ttcctattaa aagtttaaaa
     1141 gaatgtgtcg attttttaaa cgaagagata aaagtcgata aagtaaggat aatgtctttt
     1201 ttggatgata agtcaagaaa agagaacgaa gaattagaaa aagataatag ttatatagat
     1261 tgcaaatata ctaagattaa taatgaagaa agtaaatatg atgaagactt taaagatgta
     1321 aaaggaaatt attttgtaaa gaggagtgca gaaattgctg ctgctggaaa tcataatatg
     1381 tttatgattg gacctccagg ctcaggaaaa actatgattg caaaaagagt aaggacaatt
     1441 ttaccagata taagtgtaga agaaatgata gaagtaagta aggtctatag tatattgggt
     1501 atgataaatg aaactaaagg aataattgat aaaagacctt ttagagcacc tcatcataca
     1561 actacaaagc aatctttaat tggaggagga atggatgcaa gacctggaga aattgcttta
     1621 gcacatagag gtatcttatt tttagatgag atagccgaat ttgatagaaa aatacttgag
     1681 actttaagac aacctataga agatggatat gtaaatatat ccagagtaaa gtacagtgca
     1741 aaatatcctt gtagagtact gttagttgca gctatgaatc catgtccatg tggttattat
     1801 atgtcagaga cagagtgtag atgtagaagt aacgaaattg atagatacat aaataaaata
     1861 tcaggtccat tgttagatag gtttgatatt tttgtagaag ttaattctat aaaatattca
     1921 gattttaatt ctttaaaaca agaagaaagc tctcaaaaaa taaagagaag agtagaaaat
     1981 gctagaaaaa tacagataaa tagatttaag aaagataata taaaaaataa tagtgaaatt
     2041 aaagcttaca atttatttaa atattgtaaa ctggaaaaag aagcttcaaa aacggctgag
     2101 atgatattta gtaaatacaa cctaagcagt agaagttata caaaactttt aaaaatggca
     2161 agaacaatag cagatttaga agaaagagat ttgataaatt cacagtgtat tatagaagct
     2221 ttttcattta gaaaggctta ttatagttat tttaaatagt tttttgaggt ggattttatg
     2281 gaaaaaagag atacttattt atggttaaaa tcaattggtg gaataacaac aaaaacaatt
     2341 gaaataatag aaaatgaaat agtaaatatt gaagatatat ttgatttttc tgaaaaagaa
     2401 atatataatt taaaaaatat aagcttgaat attagaaaaa atatagtaaa atatagaggt
     2461 cacgcttatt tagaaaatat aaaagaacta ctttacaaaa aagcgattaa atatatttgt
     2521 aaatatgata aagaatatcc tgaaaattta aagaatatat acaacgctcc aaagttatta
     2581 ttctacaaag gtgacatagg cttagttaat aataatttta atatagctat agtaggttca
     2641 agaaaaccta cagcctatgg aattaattgt gcaaaaacaa taagttgtca actatcccaa
     2701 tatggagtta atatagtaag tggattagct ataggaatag atgcatactc gcacataggt
     2761 tgtatgagtg gaaaatctaa aactatagca gtacttggtt caggagtaga caatcctctt
     2821 ccaaaacaaa atttacattt atcaaataaa atattagaaa atgggggatt attattatca
     2881 gagtataata ttaactcgac agtagccccg taccattttt ctaatagaaa tagaattatc
     2941 agtggtttaa gtgatggagt agttgttgta gaagcagcaa taaaaagtgg tgcactaatc
     3001 acagtagatt ttgctcttga acatggaaaa aatgtttttg caatacctgg taatataaat
     3061 tctcaaatga gtagaggttg tcataaaatt ataaaagaag gtgcaaaatt aattgaaaat
     3121 atagatgata ttttaaatga atataacata tttaatatta ttgataaaaa aataaatcaa
     3181 aaatatgata atataagtct aaatgccaag tctaagcaaa taattgaagc tataaaaaga
     3241 gaaggaaact tgcatataga tagtatttgt gattatactg gtatagaaat aaagtatgta
     3301 aattcaataa tcaatgaatt agtactaaat gagttagtag tagaaatgaa taataaaacg
     3361 tacagtttaa atgtataaat taagattagg tagtgggggt gtatggtttt atggccaaaa
     3421 cattagtcat cgtagaatcg cctgcaaaag ctaaaactat agaaaaattt ttagggaaaa
     3481 gtcattacac agttaaagca tcagttggtc atgtcagaga tttacctaaa agtaaattag
     3541 gtgtagatat agaaaataat tttgaacctc aatatataaa tataagagga aaaggcgatg
     3601 taataaaaga attaaaaaaa gaagctaaaa aatctaaaca agtatatcta gcaactgacc
     3661 ctgatagaga aggtgaagct atatcatggc atttagctca tatactaaat ttagatgaat
     3721 ctgaggattg tagaatagag tttaatgaga taactaagga tgcaattaaa aaagcaatta
     3781 aacatcctag aaatattgat ataaatttgg ttgatgcaca acaggcaaga agagtgctag
     3841 acagactttt aggttatcaa ataagtccta tattatggca aaaggttaga aaaggtctta
     3901 gtgcaggtag agtgcaatct gttacaacaa agttaatttg tgatagagaa aaagaaataa
     3961 aggcatttat tccaaaagaa tactggacaa tagatgttga ggctaagaca gtaaaagaca
     4021 aagatataac tttgaaattt tatggtaaaa atgatgaaaa gattgaatta gaaaatgaag
     4081 aagctgtaaa tgaaatacta aaagaaatgg aaggtaaaga tttaaaggtt gagaagatag
     4141 aatctaaatc aagacgtaaa tcagctccaa aaccatttac aacaagtatg cttcaacaag
     4201 aaggagcaaa taagttattt tttactacta aaaaaacaat gatgatagca caagaattat
     4261 atgaaggtat cgacatagaa ggtgaaggta ctgttggtct tatatcatat ataagaactg
     4321 actctaaaag aatatctgat gaagcaaaag aaaaagctaa gggttatatc gtaaacaatt
     4381 taggagaaaa ctactataaa aacattggtg ataaaaaaga aaaaaaggat actaaaaagg
     4441 ttcaagatgc ccatgaggca ataagaccaa cctcagttga tagaactcct gagagtataa
     4501 aagcctcatt gagcaaagac cagtacaagt tatacaattt aatttggaga agatttgtag
     4561 ctagtcaaat ggaagattca gtgtttgata tactaaatgt agaatgtaaa ataggcgata
     4621 ttgtatttaa ggcaactggt tcaaaaatga aatttgatgg ttataccaag gtatataatt
     4681 tttcagaaag agaagacaaa attttaccaa gtatagaaaa aggagatata ttaaatatag
     4741 aaaatatatt gccagaccaa cattttacac aaccaccagc aagatataca gaagctagtt
     4801 tagtaaaaac actagaagaa ctaggtatag gtagaccaag tacgtatgct ccaacaatag
     4861 ctactatact gaatagagag tatgttgaaa agcaaggaac aagtctatgt ccaacagaac
     4921 ttggaataat agttactgat attttggaaa ataatttcca taaatttata gatgtagatt
     4981 ttactgctga tatggaaagt aaacttgatg ttatagaaga aggcaataca gattggaaaa
     5041 agattgtatc agaatcatat gcaccactta aagaggctat tgaagaagct cttgaaaata
     5101 tagaaaaagt aaatatggat gaagaaactg atgagatttg cgagaactgt ggttctaata
     5161 tggttatcaa atatggtaga tttggaaagt ttatggcttg taaaaactat cccgattgta
     5221 aaacaactag accaattatg aataaaattg gtgttaaatg tccaaaatgt gaagaaggag
     5281 atattatact tagaaaatct aaaagaggta aagcttttta tgggtgttct aattatccaa
     5341 actgtgactt tgtagcttgg aataagccaa caggagagtt ttgtgagaag tgtggttcat
     5401 atatggttga aaaagtgact aaatctgaaa caaaaacagt gtgttctaat aaagaatgta
     5461 ataaagagac aaaagagcat aatgacggaa aagagtgaaa attgaaaaaa atcacataat
     5521 tctcttgaaa aaggttccaa tctatgataa tctttcaagt gtaaacaact aatagaaaaa
     5581 taaattttca ttaaatgtga aaataatatt agaaaatata aaaggttatt aaaaggccta
     5641 aatttcatga ggagatgatt aaatggcaag tgaagtgtta caaaaaacaa ggaaaataaa
     5701 taaaacatta caaacaagtg gtggaagcag tgtctctttt gatttactgg ccggagcatt
     5761 gggcgacgtt ttaagttcta atgtttatgt agtaagtgca aaaggtaaag tactaggtct
     5821 tcatttaaat gatgttcaag acagttcagt tatagaagat gagtatacta agcaaaagaa
     5881 attttcagat gaatatactc aaaatgtgtt aaaaattgat gaaacattag aaaatttaaa
     5941 tggtgagaag atattagaaa tctttcctga agaacatgga agattacaaa aatatactac
     6001 agtagttcca atattaggaa gcggtcaaag attaggaaca ttggtacttt caagatattc
     6061 aaattcattc aatgatgatg atttagtaat agctgaatac agtgcaactg ttgttggtct
     6121 tgaaatatta agagcaatag gtgaggaatt ggaagaagaa atgagaaaga aagctgtagt
     6181 tcaaatggca ataggcactc tgtcctactc cgagcttgaa gcagttgaac atatttttgc
     6241 tgaattggat ggaaaagaag gtttacttgt agcaagtaag atagctgata gagttggtat
     6301 aactaggtct gtaatagtaa atgcacttag aaaatttgag agtgcaggtg tgatagaatc
     6361 aagatcatta ggtatgaaag gtactcatat aagaatactt aatgacaaac ttacagatga
     6421 attaaaaaaa ttaaaaaaca atcaataatc tatattttat aggtttagat tagaaaaggt
     6481 atctatttca aatagatacc ttttttagac gaattattaa ggtaatgata agtatattaa
     6541 aaaggagtga gaaaatgcct ttagcagata aaataagacc aaaaacaatg aatgatgtaa
     6601 taggtcaaag tcatataata ggtaatggaa aaatattgtc taagatatta caatctaatt
     6661 ttttaccaaa tatgatattt tttggtcctc ctggtgttgg taaaactaca gtagcggaaa
     6721 taatagcaga aagaagcaat aaaagcttct ataaaataaa tgctactaat tcatctttag
     6781 aagatataaa aaaggtgata tcagagcttg gaagtataaa taatgtaaat ggagtcctat
     6841 tatatataga tgaaatacaa agttttaata aaaaacagca acaatcaata ttagaattta
     6901 tggaaaatgg gagtataact ttgattgcta gcactacaga aaatccatat cattatgtat
     6961 ataaagctct tttaagtagg tctactgtat ttgaatttaa acctttagaa aaacttgata
     7021 tagaaaaagg actgaaaaga gctgttgaag tattaaatga agatagctat atggatattg
     7081 agtgtaataa tgatgctatt gaagatatag caattctttc tgatggagat atgagaagag
     7141 ccttaaatat tttggaagtg gtagtctatt caactaaacc aaataaggat aatataactt
     7201 atatagattc agatgtaata agggcatcta catttaataa aattataaac tatgacaaaa
     7261 atggtgatag tcattatgat attttaagtg catttcaaaa atctataaga ggaagtgacc
     7321 cacaggcatc tatacactat ctagcaagac ttataaaagg tggggatttg ataagtatat
     7381 gtaggagact tttagtaata gctagtgaag atataggttt agcttatcca aatgctattg
     7441 taattgtaaa ggcatgtgta gactcagcta tgcaacttgg atttccagaa gcgaaaatac
     7501 cattagcaga agctactatt cttttggcta catctccaaa gtctaattct gcatgtatag
     7561 caataatgaa agctatggat gaactggata aagagtttgt taaggatata ccaaatagta
     7621 taaaggatgc tcactattct ggttctaaag aaataggtag gggttgtgga tacaaatatc
     7681 ctcataattt taaaaatcat tatgtaaagc aacagtattt accagatagc ataaaagata
     7741 gtatttatta cataccacaa gaaaataaaa ctgagaaaaa tataaaaaaa tatttagaat
     7801 atctaaatag tgatttttag actgaacttt atgtatcggg ggtagatatt atgattataa
     7861 aacttaactc aacttatcac agtagagtta tgaaatactt aaaaaaagaa cctgagtaca
     7921 atttgtttat aataggggat attgaaagat atggatatgg aaataatttt ttaaatatat
     7981 gggcagatgt gggtgaacat ggtgaaataa aggcaatatt acttaaatat tttgaattta
     8041 tgatgtttta ttctgatgga gagtatgatg ttgaaggttt ttataatcta cttcgtaata
     8101 ctaattacga agaaatatct gggaaaatat gtgctgtaga tgctttagca aagagattag
     8161 gacttaataa tttaaaggta gttgattttt gcaaacttca aactaaaaag tttttgatag
     8221 ataataattg taatgcaaag gttaagagaa ttaggcttgg aaatctaaaa aagacagtaa
     8281 aactttatga cttaatagat gaatttcaca gtacaacttt agagaattta aaaaatggac
     8341 taaggacagg tagaggatat tgtatagaga ttaacaaaca ggtggtgtca atggcaaaga
     8401 gtacttcaga aaataggact catgcaatga taattggagt aggaacacat cctaaatata
     8461 gagctaaagg acttgctaca aaatgcctaa ttaaactatg ttcagagtta ctgagagaaa
     8521 acaaaattcc ttgtttgttt tatgataatg aagaggctgg aaagatatat aaaaaactag
     8581 gatttgaaaa cattggaaaa tggggaattt attcaaaata aaaaattaaa attaatcttg
     8641 acaaaattag tagggtataa taaaattatc ttgaaagtaa ttcctactaa aacagtatga
     8701 attagatttc tatatagagg tgataatata tgaagttgtc tactaaaggt agatatggac
     8761 taaaagcaat gtttgaatta gcattaaatc aagacaatgg accagtatca ttaaaattta
     8821 ttgctaagaa gcaaaaaata tcagatcagt acttagaaca aatattttca tctttaaaaa
     8881 aatcgggtct agtaaaaagt gtgagaggtg ctcaaggagg ttatttgctt tcaaagaatg
     8941 ctgaagacat aacagtagga gatatacttg tagtattaga aggaccagtt gcattatctg
     9001 actgtgtact agatgaagat gtatgtgaaa actcaaacat gtgtgtaaca aaaatagttt
     9061 gggaaaagat gaaaaagggt atagaagatg ttatagattc aattactctt aaagatatga
     9121 taaatgacta taacaaaaat aaattagaaa atgatataac aaatattaaa aaatagattt
     9181 ggagatgaat gtaatggaaa aaagaagatt gtatatggat tattctgcaa caacacctat
     9241 taaaaaagaa gtattggatg caatgatgcc atacctaaca gattattttg gtaatgcatc
     9301 tagttttcac acattcggaa gagaagcaaa agatgctttg gataaagcaa gagaacaagt
     9361 agcagccctt ataaatgctg aacctagtga aatatatttt acagcaggtg gttcagaaag
     9421 tgataactgg acactagagg gtgtagctta tgctaataaa aacaaaggta atcatataat
     9481 aacttcaaaa atagagcacc atgcaatact tcatacatgt gaatacttag caaaacatca
     9541 tggatttgaa ataacttatt tagatgttga tagcgaagga aaagtagact taaagcaact
     9601 agaagattca ataaaagata ctactatact tataagtata atgtttgcaa ataatgaaat
     9661 aggaactata cagcctatta aagaaatatc cgaaatagct aaaaaacata agatattatt
     9721 ccacactgat gcagtacaag ctacaggtaa tattccagta gatgttaaag aattaggaat
     9781 agatttgatg agtatgtctt cacataaaat atatggacca aaaggtgtag gagctttata
     9841 cataagaaaa ggtgtaagac ttcataactt tgtccatggt ggagcacaag aaaaaagcaa
     9901 aagagctggt acagaaaata tacctgcaat agttggatat ggaaaagcag cagaattagc
     9961 taaggaaaat atgcaaaatc atgtagaaac tttaactcgt cttagaaata agttaataga
    10021 tggtgtgtta gaaagaatac cttacacaag agtaaatggt agcttagaaa atagattgcc
    10081 aggaaatgca aactttgcat tccaatttat agaaggtgaa ggaatacttt tattattaga
    10141 tatgttagga attgctggtt caagtggttc tgcatgtact tctggttcat tagacccttc
    10201 acatgtactt cttgcaatag gtttaccaca tgaaatagca catggttcat taagacttac
    10261 agttggagat tttacaacag atgatgatat agattatata ctagagaact taccaaaagt
    10321 aatagaaaga cttagaagca tgtcaccact ttatgatgat gcgaagaaac aaggtttagt
    10381 aaaatagaca taaagataaa gcataaaaaa tagacataaa ttgagtagga ggaaaagata
    10441 atgcaatata gtgataaagt tatggaacat tttatgaatc caagaaatat gggagaaatt
    10501 gataatgcaa gtggtgtagg tgaagttgga aaccctacat gtggagatat aatgaagata
    10561 tttttagaca tagatggaga tgtaataaag gatgttaagt ttaaaacatt tggctgtggt
    10621 tcagctatag caagttcttc tatggcaact gaaatgataa aaggaaaaac tataaaagat
    10681 gcattagaac ttacaaataa agctgttgca gaagctttgg atggtttacc accagtaaag
    10741 atgcattgtt cagttttagc tgaacaagct gtaaaagcag ctcttataga ttatgctcaa
    10801 aagaacaata tccatatacc agaattggat ggatatgtta tagatgatgc tcatgaccat
    10861 gatgttgaag aagaagaata aaaataagtg attgtgttat gagaagttta caatgattaa
    10921 aaagaagtac taatgatata attaggacat aaaaaatagc tgtttttaaa atagctattt
    10981 tttattgcct aaattaagta ttttaacttg atttagaggt taattagtta acaaaataat
    11041 ccataagatt caaataataa agatgattat ttttttagaa aatagaacaa gctatatttt
    11101 aaataagtac aatacttgct ctatatggta taataataag taatgcaaaa gatgaggtga
    11161 aatttgttta tgaataaaaa agtaatgata ggtatgagtg gaggagttga tagctctgta
    11221 gctgcatatc ttttaaaaca acaaggatat gatgtcattg gtgttactat gaagttgtgg
    11281 caagatgatg atgttgttga aattgaaggt ggttgttgct ctttatctgc tgttgaagat
    11341 gctagaaggg tagcaaataa aataggtata ccattttatg ttttaaattt tagagaagtg
    11401 tttaaagaaa aagtaataga ttattttata gatgaatatt tggaaggaaa aactcctaat
    11461 ccctgtatag cttgtaataa acatataaaa tttgatgatt tttataagaa ggctagacaa
    11521 ataggttgtg attatgtagc tacaggtcac tatgcaaaga tagaaaagga tgaaagtaca
    11581 ggaagatatt tgctaaaaaa atctgttaca gataaaaaag accaaactta tgcactttat
    11641 aatcttacac aagagcaact agagcatact ttattgccaa taggtgacta tgaaaaagat
    11701 agagtaagag aaattgctaa ggaaatgggt atggcagtac acaataagcc tgatagtcaa
    11761 gaaatttgtt ttgttaaaga taatgactat gcaaattatg taaaaaaaca ttctaaaaag
    11821 cgtattgaag aaggtttttt tgtagatact aaaggcaata tacttggaaa acataagggt
    11881 atattatatt atactatagg ccaaagaaaa gggcttggaa ttacttttgg aaaaccaatg
    11941 tttgtaatag acataaatcc aataaataat accatagttc ttggagacaa tgaagattta
    12001 tttaaaaagg aacttattgc taaagatgtt aactttatat caatagatac gttagaagaa
    12061 cccttaagag ttcaagccaa aataagatat tcagcaaaac catcaccagc tactattcat
    12121 agagtaggag aagatactat taaaatagtt tttgatgaag ctcaaagagc tattactaaa
    12181 ggtcaatctg tagtaatgta tgatggagat attgtagtag gtggaggtat aatcgagaaa
    12241 agtttataaa aacatctaaa aaattaaaaa aataagcaat aaagtatatt aagagtaaat
    12301 aaagtttata aaataaattt attgtatatg aaaatatagt attgattaat tttgtaaaat
    12361 ttagtaatat aattagtata ttaattaaaa aacaaataca gtgataagga atagtaaaat
    12421 atttgatgtc ttacagagag agggaaatgc tggaagccct tagaatatga tatttgaagc
    12481 agccttttag tagcaatctt gaacaattta tcttttataa agaagattta agtagagatt
    12541 gacggtagtt acgttatagc taaggttttc cgaagatatc atactttgta tgataattag
    12601 ggtggtaccg cgattgtcct cgcccctata aatttattta tagggacgag gttttttatt
    12661 taaacgaaaa aataaaattt attaggagga acatgatatg gaaaagatgg gattaaatga
    12721 aataagaagt aagtttttag aattttttga gtcaaaaggt cattatgtag caaatagtta
    12781 ttctttagta ccaaacaatg ataagagttt gttacttata aactcaggta tggcaccttt
    12841 gaaaaactac ttttcaggtg tagaagtacc tccaagtgtg agaatgtgta catctcaaaa
    12901 atgtataaga acaggagata tagaaaatgt aggtataact gcaagacatg ctacattctt
    12961 tgaaatgatg ggtaatttct catttggaga ttactttaaa agagagtcaa taaaatgggg
    13021 atgggaattt gttacagaat ggctaaatat accagaagat aagatatggg tgactgtata
    13081 tgaagaagat gatgattctt atgatatatg ggcaaaagaa atgaatttcc cagaagaaag
    13141 aatggttaga cttggaaaag atgataattt ctgggaaata ggtacaggtc cttgtggtcc
    13201 ttgttcagaa atatattttg atagaggaga agaatatgga tgtgacaatc cagactgtaa
    13261 accaggctgt gattgtgata ggtatttaga attttggaac catgtattta ctcaatttga
    13321 tagagatgaa gaaggaaatt atagtctatt agaaaacaag aatatagata caggtatggg
    13381 tcttgaaaga atgggatgta ttatgcaagg cgtagacact atctttgaag ttgatacaat
    13441 taaatctata ctagaagcag ttgagaagtt aacaggagta aaatatggag agaatccaaa
    13501 aaatgatata tcaataagaa taataactga ccatataaga gctgtaacat tcttagttag
    13561 tgatggagtg cttccatcaa atgaaggaag aggatatgtt cttagaagac tccttagacg
    13621 tgctgctcgt catggtaagt tattaggagt aaaagaatta tttttacaga aactaataga
    13681 tgaagttata aaagtaaatg ataaagcata tccagtttta gttgaaaaag aaagttatat
    13741 taaaaaagta gttggaatag aagaagaaaa attcaatgaa actatagacc aaggaacaga
    13801 aatattgaac tcttatatag aagtattaaa aaatgaagga aagactgtcc taagtggtca
    13861 agaagctttt aaactatatg atacttatgg cttccctata gacttaacta aagaaatatt
    13921 agaagaagaa catctatctg tagatgaaga agcttttaat gaagaaatgg aaaagcaaaa
    13981 agaaagagct agaaatgcta gaggaaatat ggatggagaa agttggaagg aagaccctct
    14041 atcaaaatta gaatctacag ttgacagtac atttaatggg tatagtgaaa tttatggtga
    14101 aggaactatt gaagctatag ttaaagatga tgagctagtt caaagtgcag aagaaggcga
    14161 taaggtatct atagtactag ataacactac tttctatcct gaaggtggag gacaagttgg
    14221 agattgtgga ttgataacta atgaaaactt agttttagaa gtattaaata ctaaaaaagg
    14281 tgctaataat agtataaaac atataggtat aataaaatca ggaagaataa gtaatgggga
    14341 caaagtgaag acactcgtag atagagaaac tagaatgtca gctgcaagaa atcacagtgc
    14401 tacacattta cttcataagg cattaagaga agtattagga gaacatgtta atcaagctgg
    14461 ttctttagta actccagaaa gacttagatt tgatattact cactttgaag cgatatcaaa
    14521 tgaagaatta aaagtaatag aagaaaaagt aaataatgtt attttatcat ctttagacat
    14581 aaaatgtgat attatgaata taaaggaagc taaagaaaaa ggagctacag cactatttgg
    14641 tgaaaaatat ggtgatgagg taagagtagt ttctatggga gattattcta cagaactttg
    14701 tggaggaact cacttgacaa atacttctca agtaggaatg ttcaaaatat tatctgaagg
    14761 tggagtagca gcaggtgtaa gaagaataga agcaataaca ggaaaagctg tttatgaata
    14821 cctaaaagaa agagatggaa ttatctctga agtttgtgta aacttaaaat ctaaagaaga
    14881 taatttaatt caaagaataa gttctttatt agaagaaaat aaaaacttat ctaaagaatt
    14941 gcatgatatg aaagctaaga tgagtttaca atcagttgat tctatttttg attctaaagt
    15001 tgaagtaaat ggagttaatt tgataactaa taaatttgaa ggtatggata tggatacact
    15061 aagagaaaca gcagataatc taagagataa gcttggttct ggtgtagtag tacttgcaaa
    15121 tgtagtagat gataaagtta actttgtagt tactgccact aaagatgtat tagataaggg
    15181 aatacattca ggtaatattg ttagagaagt tgcaaaaata gctggaggaa aaggtggagg
    15241 aagacctaat atggcacaag caggagctag tgatgtatct aaggttgacc aagctttaag
    15301 ctatgcaagt gaggttatta agacacaagt taaataacat tcactcaagg aggtttttag
    15361 tttggaaaaa gatttagact acactatgaa atttgaagga ataccagaag ataggatgag
    15421 tgtaggagat actattgatt ttgtatataa agcattagtt gaaaaaggat ataaccctat
    15481 aaatcaaatt atagggtatc tcctttctgg tgattctagc tatataacta gtcataaaaa
    15541 tgcaagagct ataattaaaa aatttgaaag agatgagata cttgaagaag taatcacgca
    15601 ctatctgaat agaaagtagg tcaaataaat gagaaaaaag attatgtcgt tgtttgcaat
    15661 agtcattatg atttttgtat caactgctag ctatggtttt gctgaagaaa aaggcaaggt
    15721 catatttatt gatatgaata ggaccaatct tggaagtatg atgaatattc caatcttgaa
    15781 aaatgagata gaaaagagag gctatgtagc tctcatgaat ataagagggg atcaaggtac
    15841 tgatgataga aggtcttatg caagtatggg tgcaggaaca agagctagtg taactactga
    15901 agactatatc aattttcaaa aagtagacca agatactgct aaagcttttg aagtcgcaac
    15961 aggtcaaaaa gctaaagaaa taaatgattt aggcattaat agatctataa atgaaaatct
    16021 tgagtatgga gaatatggag caaatttagg gtcattagga aaaactttat cagaaaatgg
    16081 tttaaagaca tctgtaattg gaaatgctga tataattgaa aataatcaat tagttaaaaa
    16141 cagaaactta tgtttagtag ctatggacca atatggaagg attgattcag gtaatattga
    16201 taatattaat aagaaagatt ctaaaatgcc atttggaata tctacagatt atgataaatt
    16261 actagatgaa actaaaaaat cctataaaaa tagtgatgct atatttgtag aacttggaga
    16321 tacatataga ttagatatgt atagattaaa tctaaatgat aatacttatg atataatgaa
    16381 gaaaaatatt gtaaaaaata taaataaata tcttgaacaa gtattttcta tggttggaga
    16441 aaatgacact gtttatatag caagtgcatt tccaagtgac ctagattata agaataagag
    16501 aagactttct ccaataataa aatttagtgg agaagggaaa ggtctactgg aatctgccac
    16561 aacaagaaag gctggaattg ttgccaattt agatgttgga gtagatatac taggtacatt
    16621 tggtcttgaa aataaagaga tggtaggtag aagttatact ttagtaaata aagaggacaa
    16681 tattagctat ctaaaatcac tttatgaaag gatagtgtct atatcttcaa taaggtcaac
    16741 tattataaat ggctttgtag gcgtaatatc tgcttcttgg gtaattgcca tgtttgcgat
    16801 tttctttaga aattatatgc cacataaaga gcgtgtattc aaagtactta aggagtttat
    16861 aaaattaggc ataataatgc cactatcatt tatgttagct cctttgatga attttaaaac
    16921 tccaatttca atatctttgg gaatatttgc aactacgcta atattatact tgttaggtag
    16981 aaaattattt aaggacaatg atataaagca catgggattt tttgcaacac ttactatttt
    17041 agtcatagtt atagattctg tatttggaac atacttaatg caaaataaca taatgagtta
    17101 tgatgctgtc ataggagcta ggtactatgg tgtaggaaat gaatatgaag gtgtaactat
    17161 agcaagtgca gtatttggct tagcagtatt attacattat aagaaggtat ctaaattact
    17221 tgttgtaatt ttttcattaa taatacttat aactagtgca tatccttcaa tgggtgcaaa
    17281 tgttggtgga gctatatcag aatgtgtagc atatttgttg tttataatgc ttatatatga
    17341 cattaaaata gattttaaga aagctgtatt attagctgtt tcagctgtag ttgtcgtttt
    17401 tgcatttgca gctttagata tactttcagg tagtgaatct catttaggta tgtttgtaca
    17461 gagaattttc ctaaatggac caggagagat attacaaaca tttggaagaa aaattcaaat
    17521 gaatatgcaa ctagcacaga caagtgtatg ggtaaatatt ttacttgtag gaattggagt
    17581 aattgcagtg cttatattta aaccatctag acactttaga aagataatga ataattttcc
    17641 aatcttgttt aaagggttta ttgcatctat ggttggttgt ataattacac ttttagttaa
    17701 tgattctgga atagttgctg catctacagc atctatatat atacttatac cactaataat
    17761 aataagtata aatatgataa tatttgaaaa aaataactaa aaaaattttt actgttcata
    17821 taggatttaa ataaaaaatg caatatgatt attaatgtaa actggatgct tacataaaaa
    17881 atcatattgc aatttttatg attgattatg taaaattata tgaagcagta agcctgtgac
    17941 cttagttatg agaggttcag aatatgtatt taatctatag aatataaata gataaaggag
    18001 gaactatgtt agatggaaga ataatgggtc ttgatgttgg tgataaaact attggagttg
    18061 ctgtgagtga tttgatggga ttaactgcac aaggtgtaaa aacaataaaa agagttggca
    18121 agaaaaaaga tattgaagag ctaaaagcaa taataaaaga aaaacaagta aataaaattg
    18181 tatcaggatt accaaaaaat atgaatggaa ctttaggtcc acaaggagaa aaagtaatca
    18241 agttttgtga attggtaaaa gcagaaacag gtattgatgt agagttttgg gatgagaggc
    18301 tttctacagt agcggcagag aggtctttat tagaagcaga tgttagtaga caaaaaagaa
    18361 aaaaggttat agatatgttg gcagcagtaa taattttgca aggatattta gattttaaaa
    18421 taaattcata aaagaactat aaaattgaat acttatgata tataatataa ataataaaaa
    18481 taaaaataaa tttgaggtga atgacattat ggaagaaaat ataataaatt taatagacga
    18541 aaatggcgtt gaaagccaat ttgaaataat attaacatta gaagcagaag gaaaagagta
    18601 tgcaatatta atgcctttag atgatgaaga agctgaagaa gctttaatct ttagaattga
    18661 tgaggatgaa gaaggtgaaa tcttaatacc tttggaaagt gatgaagagt atgaaactgt
    18721 tgtagctgtg tatactgcta taatggaaga agaaggttta aattacgacg aagatgaatc
    18781 aaatggactt aactagataa gtaattaagt ttgtatataa aaatcagata tttattatat
    18841 ttgctttaat taaacattat taattgtaaa agcatttata gtatatatct gtttttttgt
    18901 tttttattaa aaataccaaa aaatttatga attttggtga ataatatatc ttttatgggt
    18961 atatatttat attatgagaa taaattaaat ttatttaaat gtcttgacaa aaaatgaatt
    19021 atcaattaca atattattta ataaagatta tcaattgaaa atagtaaggg tgatgttatg
    19081 gcaaatacaa tggatttatt aaaagacaag ttaaaagaaa ctggttttaa gattacgcca
    19141 caaagaagag caatagttga aatattgctt aaacatgacc attcacatct aagtagtgag
    19201 gaaatctatg atttagttag ggttgactgt cctgaaatag gcttagctac tgtatataga
    19261 acaatgcagc tattggatga aataggatta atatcaaaac taaacttaga cgatggatgt
    19321 ataagatatg agattagtct acataaagaa gactgtcata atcatcatca tcttatttgt
    19381 aaaaattgtg gaaagataat ggaagctaaa gaagaccttt tagacaatat agaaaaagag
    19441 atacaatcat tgtacaaatt taaaatactt gatcatgatg ttaaatttta tggactatgt
    19501 gacgagtgta atggagtaag cgattcagaa gaataaactt ctattgctta atgtcgatgg
    19561 aagaatagta tagaactttt atattctatg ctattcttcc attttttatt ataaaccatc
    19621 tataaaatgc aaatatacag ttttcaagat gcttatagtt ttaatattga aaatataatt
    19681 aatcctataa gtacaaaaat gaatgatttt tctatgaaat tactaaatgg tcctccaaca
    19741 gttaaaaaac ctaaagaaat tttcttttcg cttagcggat aaaaaattgg tattccttgc
    19801 tttgtaaata aatctcctaa aaacatatga cttgcataac caatcgtacc ataaaaagat
    19861 aaattattaa tattagtgat taattctatt tgttttagta aaaaatatac tattatagtt
    19921 gcaaatatgc tatgagaaaa acttctgtgc tttaaccagg gaaaaattga caacattaga
    19981 caaaaaattg taaaatatat ttcaacttct atgatgtata agcatatagc taatagtata
    20041 aatattaatg atagaaaaac ttttcttaga aatgtgtggg ttagttttgc ctcaataatt
    20101 ataattgcaa caaatgtaat tattgcgctt aaataaaaat tatcatttat atttattgaa
    20161 ataaagaaaa taataatatt aacagcatat ataaaaattc tatatatgta tttggataca
    20221 ttgtttttta gaatgaaatt tgagaccata gaattagatt tatccaaatc gggtagtaca
    20281 gcaaaagtag ctgaaactaa aatatcaact aaagatatag gaattttaaa tattaaagat
    20341 gtttggatag ctgttaagat tcctattgca caatgagttt taccacgcat aaatcctcct
    20401 aaattatccg atattaaatc atataatatt tagaggatat caaaataaaa taggtttgtc
    20461 aaaataatac gttaaatgta ttttaaatat agcttaaaat atgatacgaa ttatggtaca
    20521 ataagaataa ctggtagcga gtgcgtgatt tgatattatt tatgattgta gaacgtgtta
    20581 aaaaattaaa tagacagaag gagatgatgc aattgttcaa aaagaacacc aacaagataa
    20641 aggtaatggc cttgggtgga cttaacgaag ttggaaaaaa tatgactgtt gttgagtata
    20701 aagatgaaat aattgttata gatgctggac ttagttttcc agaggatgaa atgttaggag
    20761 tagatatagt tataccagat ataacttatt tagtaaaaaa tagggataaa ataaagggta
    20821 tatttattac acatggacat gaagaccata taggagctct tccatatata ttaaagaaaa
    20881 taaatgtacc tgtatatggg gcaagactta gtataggtct tatacaagta aaacttaaag
    20941 aacataaaat gaataatgtt aagttaaatg ttataggtcc tagacaagtt ataaagcttg
    21001 ataatatgga agtagagttt ttgaaaaata accatagtat accagatgct tactctattg
    21061 caatacacac agaccaaggt ataatatacc acacaggaga ttttaagatt gacttgactc
    21121 ctattgatgg agatgttatg gatatgcata gaatctgtga gttgagcaaa aaaggagttc
    21181 ttttaatgct ggcagatagt actaatgcag aaaaaccagg atttactatg tctgaaaaga
    21241 ctgttggggt aggtcttgac gaattatttg caaaaggaaa tggcagaaga attatagttg
    21301 ctacctttgc atccaacata cacagattac aacaaataat aaatactgct gaaaagttta
    21361 atagaaaagt tgctatatca ggtcgctcaa tggtcaatgt agttggagtt gcaaaagaat
    21421 taggatacct agatatttct gatgacatgc ttatagattt aaatgatata tgtaagtatg
    21481 aagatagtga attagtgata ataacaactg gttcacaagg tgaacctatg tcagctcttg
    21541 ctagaatggc tttttcagag cacaaaaaag tagaaataaa gagtggcgat ttagttataa
    21601 tatctgctca tccaatacca ggaaatgaaa aattaatatc aagagtaatc aatttcttat
    21661 ttgaaaaagg agcagaagta gtatacagtg atatagctga tatacacgtt tctggacatg
    21721 cctgtcaaga agaattaaaa cttatacatg cattagttag accaaagttt tttatgccag
    21781 ctcatggtga atatagaatg ttaaaaagac atgctgaaat agctgaacag cttggtatgg
    21841 ataaagaaaa tatttttgtc atgcaaacag gagatgtttt agaattagat aaaaattctg
    21901 ctaaggtagc aaaccgtata caaacaggaa acatattagt tgatggtcta ggagtaggcg
    21961 atgtaggtaa tatagtttta agggatagaa agcatttatc agaagatggt cttatgatag
    22021 ttgtagttac tatttccaag gatgagggta aggtcttagc aggtccagat ataatttcca
    22081 gaggattcgt atatgtaaga gagtcagaag atttaatgga tggagcaaag gatataatta
    22141 agaatgttct aaatgaatgt gaagagaaaa atattaaaga atgggcttat ttaaagaata
    22201 atattaaaga aaacctaaaa gaatatttat atcaaaaaac taaacgaaat ccaatgatac
    22261 ttcctatcat aatggaagta taatataaaa aaactataaa tttgaaaatt agacctcttg
    22321 taatttttaa agtaatatca aaaatccaat tgatattatc ttaagaatta caagaggtct
    22381 ttttaatact cattttaaaa aaatatatat tacataaaat accaattata acaatctttt
    22441 attagtaaaa actacttata tataaatttt actgatagga ggattcttat ggatgatgtt
    22501 tcaagacaaa atgctataaa agctttaaaa caaacaaaaa tggaaatagc aggagagtat
    22561 ggaatgaatt atgaagatgc ttttgaaata atagaaaatg cgtctaataa aggtgtttta
    22621 gaaggttact ttaaaaagct tgaaaagaaa aaaaatttag gtcaaggtat atctaggcat
    22681 ttagaataaa aaaagttaaa tgtgagaaaa taagtttaaa taaactttaa gaggtgacat
    22741 cgatgaaaaa attagctagt actgctttgg ctatattaat tgcgcttact cctttaagct
    22801 tttcttttgc aaataataag gaaaatgctg atgccaacca actaaatata tcttcaaaat
    22861 cagcaatttt gatggatgtt ggtagtggac aaattttata tgaaaaggat gctcataaaa
    22921 agcttcctcc tgctagtgta actaaggtaa tgactatgct acttatagta gaagctttgg
    22981 attcaggcaa gattaagctt gatgatgaag ttcaagtaag tgaaactgca tctagtatgg
    23041 gtggtagtca aatattccta gagccaggag aaactcaaaa ggttgataca ttattaaaag
    23101 gtatagctgt tgcttctgca aatgatgctt gtgttgctat ggctgaacac ttagctggaa
    23161 gtgttgaagg atttgttgat agaatgaatg ctaaagcaaa agagttaaat atgaatgata
    23221 ctcattttgc aaatacaaat ggacttccag ttgcaaacca ctatacatct gctcatgata
    23281 tagctttaat gtctagagaa ctattaaaac acgaaatgat aagtaaatac ttaactacat
    23341 ggatggataa ggttgtagta gggaagaaac aagttacagt aggtcttgca aatactaata
    23401 aacttataaa gcattatcaa ggagcaactg gtgttaaaac tggttttact caagaagcaa
    23461 aatactgttt atcagcatct gcaaagagag gtaatactca tctagttgct gcaactttag
    23521 gagcagagac ttctccagaa agatttaatg atgcctctag ccttttaaat tatggatttg
    23581 ctaattatga aagtgttaag ttatgtagta aaggtgataa tatagcaaca cttacattgg
    23641 ataaggctga tgaaaacaaa gttaaattgg ttgctaaaga agatttaaat gctcttatta
    23701 aaaaaggaag ttctaaagaa tttgagaaga aaattgaaat agtaaaaaat cctaagatgc
    23761 caattaaaaa gggaactgtt ttaggaaaaa taaagatatg taaagataaa aaagtaatag
    23821 gcgaagtgga attgataaat actaaggata taaataaggc aagctatcta caaatgcttc
    23881 aaagaataat tgataatatg atataaataa ttatcagatg aaatataatt ataatacaca
    23941 aagatatgat tataatacac ataaaaaata cccttatgta attatttgcc ataggggtat
    24001 ttttatttac catatagaag tattttataa tcttatgatt tttatagaaa tatagaaaag
    24061 tttatgtatc ttaaaatatc atttgattta atatcatata tagggttgtt taacaattat
    24121 ctaagtaaag gcatactacc agtaagttta ttcactttct ttttattccc ataaattgct
    24181 atcccaaaat actgtaactc atcttctgga acactagcaa tcttttgtgt aaattcttca
    24241 taaagattgc aactttgtgc tacatctgaa aaatctacaa cagttaaatc gttaaattcc
    24301 aaagtatata attttttacg taaatctttt attatttctt tgtttccttt taaaataggt
    24361 acaggaatgt caataattcc taagtggcta ttacctgttt tgtcaattac atctgggcca
    24421 acagtttctg gggcatgttt tcctagtgta attcccatta tagctgctgt attggatata
    24481 attcccattg gtaaattttc gtctattacc atgacacatt ttgaattttt agtttccatt
    24541 tttatttctc ctttttacct tatttaaagt tgtttgaatc atcatttata aatatatttt
    24601 gatactgctt tggtgtaaga ccaataaaat tcttaaaaaa gtttgtaaag tgactttggt
    24661 ctacaaatcc tgtttgtatc gcagcatcta taggttctac gcctttttct aaaagctttt
    24721 tagctttgtt tacccgtata ttttctaggt atctatatgg tgtaatacct tttaatttcg
    24781 taaaattacg aagtagagag tacttattca tacctgctat agtgcttaat tcgtcaagta
    24841 ctatatgttc agcataatta ttttctagat aatcacatac tgcttgaatt tctatatttg
    24901 tattttccaa gttagattgc aaatttggct ctgtatgttc ttcaattaat tgttcaatta
    24961 ggaagaaaaa aagctcttcc tttttaaaat ccaattcttc ttccatgatt atatagtgta
    25021 attcttgtag tagaggtact agctcactgc gaaatataac aggttgatta aattttggta
    25081 aataattttt acctgtaatt tcaaagacag tttttttcat tatttctggc ttaatattaa
    25141 tacatctgta atcaagtact ttatcatcta tttgttcaca agtatgacta tcaaaagggt
    25201 taaaaagtag caaatctcca gtacttgtgg tatactcttt atttttacaa gataaatatc
    25261 tttgaccttt ttctataaaa ccaattacat aatgttcatg aaaatggttt gggaattttt
    25321 gcattatgcc tttaaatcta tatgcttcaa tttttaattc cctatcaaag catactgtgc
    25381 gttcttcttg tctcacttag gtaacctcct ttcatgttat acaaagtgta acacatattt
    25441 ttttatactt cttgtataat attgactaag agacaataat gtcaattcat gtcaatataa
    25501 tattacactt acagtaggtg taattattat attttcacaa tgaatatgat ataataataa
    25561 aagttataaa ataaaaagca aattttaatt ttatgcctag aagttaaaat tattataatg
    25621 taaaataatt ttgattataa gctatttaat tgttataata atattaaaaa tttaatttag
    25681 gagatgtaca ttaatgttaa gtatgaatat aggagaaata gtagcaagtt tagttggtat
    25741 agctatggca atatcaatac atgagtttgg ccatgcttat tcagctcact tattgggaga
    25801 tgatacagct aaagcttatg gtagaatgac attaaatcct gtaagacatg ttgacccaat
    25861 aggtcttttg gctatgttta tccttaagat agggtgggca aagccagtac cagtaaatcc
    25921 aaataacttt aaaaactata agataggaaa cctgatagtt tcacttgcag gcgttttttg
    25981 taatgttttg actgctatac tgtgtgtaat tatcaataag tatgtgcata tgtatgctat
    26041 aaatacaata gctgaatatg tgtttttata tagtattgga tttgcagctt ttaacttact
    26101 tccgatacct ccacttgatg gatggggagt tatatctaca tttgtaccat ataaatggaa
    26161 tgaatatttg tataaatttg aaagcatgag ttatataatt ttattaattg ctttatttac
    26221 tggtatgtat agaataatat taacaccaat atatgcagtg ttgctaaaaa tagtatattt
    26281 atttgtttaa tataataaac agattaaaga gggattgata tgaagtataa tatacaatta
    26341 caagtctatg agggaccatt agatttactt tatgatttaa taacaaagca taaaatagat
    26401 ataaaggata tatccataat agatataact aagcagtatc taaattattt gaaaatgcta
    26461 gataaaatgg atttagagat aactagtgaa tttataacta tggcatcaaa acttttagag
    26521 ataaagtcta aatatctgct ttataagcaa aaagatgaag aagaagaccc aagaatagaa
    26581 cttatggaaa agttagaaga atatagaaag tttaaggtag catcacaaga tataaaagaa
    26641 aatataactt atgttaatga gagattttat agaaataaag aagagataat tattgatgac
    26701 aatgtggatt tagaagatat atcaatagaa gcaataaaaa atatacttcc atacatattt
    26761 aaagttaaaa catcacagat agaaaatgct aatgatgaga aactagataa aatagtaagg
    26821 aagaaaataa tatctgtaga agaaaaaata ttatatataa gagatattat aaaagagaag
    26881 attgaagtta cttttacaaa tattataaaa tcatatgaaa atgatgaaat aatagctact
    26941 tttttatcta tattagagct tataaaagaa aaagaaatag tcgtagtaca agatatattt
    27001 tttgatgata ttctaataag aaaaagttcg gagtgttaaa atgaaaagag aagatataaa
    27061 gtatattata gaatctgtta tgtttgcata tggtgaacca ataagtataa aagaattaaa
    27121 ctatataata aataaagaac tttcatcaaa agaaatagag attatgttaa atttacttat
    27181 tgaggagtac agagaacaaa atagaggtat tcaaataatc aagttagaga ataagtacca
    27241 aatgtgtaca aataaagact atgcagaata tataaagaag attattgaac caaagaaaaa
    27301 gaaaagtttg agccaagcta cattagaaac tcttactata attgcctaca aacaaccaat
    27361 aacaaaggta gaaatagagg atataagagg agtaaaatgt gataaggtat tacaaacttt
    27421 gtttgaaaat gaacttataa gagaagctgg tagattgaat aagataggga aaccaataat
    27481 ttataaaaca acagatgaat ttttaaagtt gttgaatata gaaagtcttg aagaattgcc
    27541 acctatagaa aattatcaag aagtggcaac aaatgaataa aaatataaaa tcacaataat
    27601 ttagtatgta attttaaatt ttaaaagatt taaaatgctc taagttgttg tgttttt
//
LOCUS       DAYASV010000041        27514 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYASV010000041 DAYASV010000000
VERSION     DAYASV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27514
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.041"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(186..995)
                     /locus_tag="ACGI68_001731"
     CDS             complement(186..995)
                     /locus_tag="ACGI68_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0138173.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(1079..1612)
                     /locus_tag="ACGI68_001732"
     CDS             complement(1079..1612)
                     /locus_tag="ACGI68_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0138174.1"
                     /translation="MSGAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(1762..3111)
                     /locus_tag="ACGI68_001733"
     CDS             complement(1762..3111)
                     /locus_tag="ACGI68_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HFH0138175.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSITSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(3123..4505)
                     /locus_tag="ACGI68_001734"
     CDS             complement(3123..4505)
                     /locus_tag="ACGI68_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HFH0138176.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIESGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            4749..5963
                     /locus_tag="ACGI68_001735"
     CDS             4749..5963
                     /locus_tag="ACGI68_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0138177.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            6140..8053
                     /locus_tag="ACGI68_001736"
     CDS             6140..8053
                     /locus_tag="ACGI68_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138178.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKLSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            8324..9298
                     /locus_tag="ACGI68_001737"
     CDS             8324..9298
                     /locus_tag="ACGI68_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFH0138179.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            9319..9594
                     /locus_tag="ACGI68_001738"
     CDS             9319..9594
                     /locus_tag="ACGI68_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0138180.1"
                     /translation="MNAIIHANEKDNLVTCVRPLIKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            9607..10770
                     /locus_tag="ACGI68_001739"
     CDS             9607..10770
                     /locus_tag="ACGI68_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0138181.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VNYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            11107..13386
                     /locus_tag="ACGI68_001740"
     CDS             11107..13386
                     /locus_tag="ACGI68_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0138182.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(13481..14821)
                     /locus_tag="ACGI68_001741"
     CDS             complement(13481..14821)
                     /locus_tag="ACGI68_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0138183.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEDILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQDMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(14833..15615)
                     /locus_tag="ACGI68_001742"
     CDS             complement(14833..15615)
                     /locus_tag="ACGI68_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138184.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(15630..16652)
                     /locus_tag="ACGI68_001743"
     CDS             complement(15630..16652)
                     /locus_tag="ACGI68_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0138185.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(16658..17671)
                     /locus_tag="ACGI68_001744"
     CDS             complement(16658..17671)
                     /locus_tag="ACGI68_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0138186.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(17799..18737)
                     /locus_tag="ACGI68_001745"
     CDS             complement(17799..18737)
                     /locus_tag="ACGI68_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0138187.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEAKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLAKPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            19007..19471
                     /locus_tag="ACGI68_001746"
     CDS             19007..19471
                     /locus_tag="ACGI68_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138188.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(19583..19900)
                     /locus_tag="ACGI68_001747"
     CDS             complement(19583..19900)
                     /locus_tag="ACGI68_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0138189.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(19913..20644)
                     /gene="chbG"
                     /locus_tag="ACGI68_001748"
     CDS             complement(19913..20644)
                     /gene="chbG"
                     /locus_tag="ACGI68_001748"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFH0138190.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLLSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(20670..21980)
                     /locus_tag="ACGI68_001749"
     CDS             complement(20670..21980)
                     /locus_tag="ACGI68_001749"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFH0138191.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(22077..23390)
                     /locus_tag="ACGI68_001750"
     CDS             complement(22077..23390)
                     /locus_tag="ACGI68_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0138192.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(23412..23723)
                     /locus_tag="ACGI68_001751"
     CDS             complement(23412..23723)
                     /locus_tag="ACGI68_001751"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138193.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(23923..24627)
                     /locus_tag="ACGI68_001752"
     CDS             complement(23923..24627)
                     /locus_tag="ACGI68_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0138194.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDVRVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCGYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(24718..26079)
                     /locus_tag="ACGI68_001753"
     CDS             complement(24718..26079)
                     /locus_tag="ACGI68_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0138195.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            26578..>27514
                     /locus_tag="ACGI68_001754"
     CDS             26578..>27514
                     /locus_tag="ACGI68_001754"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0138196.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYII"
BASE COUNT         9704 a         4522 c         3515 g         9773 t
ORIGIN      
        1 ttaatacatt tttaagaatg acaaggaata aattataaca aattttagga atatataata
       61 atagggtctc agaacttagt tacaagttct aaaaccctat ttgtttagtg taaaaaacat
      121 cataaaattt agcttaaatc ttttcacata atattactct ttttctaagt gataataaaa
      181 tatatctatt cctctaaaaa ctgtaaaaat tcctgattaa atagtttaag ctcatcatag
      241 aatatagcat gaccactata ctcaaaagta tataaaattg aatcttctat tttttcattc
      301 ataaaaactg caaattcata cggacatatt tcatctaatt tcccatgaaa tataccagta
      361 ggcactctta cacattttaa atcattaaat aattcttcat ctcttaaaga tacagcagtt
      421 ccaatagtgc caatacctga tgcactaaaa ccaatatcat taaaccatct tctaaagctt
      481 tctggtggat ttgatgcaaa tactttttcg ccaaaatcgg ttaccatttc tggtctatca
      541 gtacatgctt gtgctatcaa tttatttaca tcttcacgtg tcataccata tggaaattct
      601 ggaggtcttt gtacaaatga tggtgcagca gctgcagcta ataccaattt tgacactcca
      661 tatccattaa ataaactcat atacctaagt acgattgctc ctcccattga aaaaccaaca
      721 agggtaaagt cttttaatcc aatagcatgt accaccttat aaatatcgtc tgctaattgg
      781 ctataagtat atcctcctga tgttgcatca gatttaccaa aacctcttaa atcaattgaa
      841 actgttctat atccgagttt tggcaatata tttgtttgat attcaaatat cttatgtcct
      901 aagggccaac catgtataaa aagtacagtt tttttagcac tcggattcaa atcatatact
      961 gctatgttta tatcattcac atatacataa tacatatcaa cagcaactcc cttcaagaaa
     1021 atgtatgatg gttattttat atatatgaat aaaatgatga ataactaaat tttcaaattt
     1081 atttacaata cttctctatt gccttttcaa ataaacttac atgaagtttt tcatctagga
     1141 ttattctatt taatatagct tttatataag ggtcttcaat aagacttata tctttataat
     1201 aattattaat agccacatgc tccaaatgca aatctagttt taatctgtta caaatagaat
     1261 ttccataaca tacaaaacca ccattccaat atgcaccaca agaacttgta gaacctctat
     1321 atacaggatt tccacccaat aatcttatag tctttgctaa tatctccata tgtaacattt
     1381 ctgttataga tatatttatc cacatttcac ttaactctct atgcgtttca tctaaatcaa
     1441 aattatggta tagatattga tttacactcg taaattcact tgatactcca gcatagtcat
     1501 ccatcaataa ttctgcataa tacttatttg gacctaaaac ttttatctct ggatatggct
     1561 catcacttga atatccattt cgcttgtgca tgtcataatg tgcaccactc ataatatatc
     1621 ctcctttaaa tttaatgtat gatattaatt gtatgcatca caactttttc atataactta
     1681 gataataaaa tctacttaaa taataaaaag ccatattaat taaaattaac atgacctttt
     1741 atccacaaaa ttacttttta attattcatc ttttccagct actgatagct ctttaataac
     1801 aactgaaggt gaacctatac tactcattgg aaattctaaa tcttctccta tagcttcaat
     1861 atctttcaac aggtcaaaat agtttcctgc tactgtaatt tgctccactg gaaatacttt
     1921 ttttccttct ttgatataaa agcccttggc agctaaagaa aagtctcctg ttactgaatt
     1981 tgcacctgaa tgaagtcctg caaagctagt aaccatcaat ccttcatcta tatcctttat
     2041 aatttcatct aaagatttat ctcctttctc tatataaaaa ttagttggat atatgcttat
     2101 aggtgatgaa tatgaagatt ttattccatt tcctgtagtc tttacacctg ccttattagc
     2161 cgtttttaag ttatgaagta aagttataag ctttccattt aaaacaactt cttttttaaa
     2221 tgtagctact ccttcatcat caaaaggagt agatgccata ccattttcta aaagtgggtc
     2281 atcaactatt gtaactatag gagatgctat catgtctcct tctctatttt tcaataaaga
     2341 taatcctttt tgagctgaat ctgcattaaa tatcccagaa aatgtgctaa gcaaagatac
     2401 catagcttca ttaagcaata tagttttata tttacctgat gtaatactct taccacctac
     2461 tttagataaa gcattatcaa caccaagttt tgctatctca caaggtttta tttcttcaat
     2521 agaatttgct atattatatc ctattccatc atatttttgt ccattgtctt ctatgatagg
     2581 aactacaaac cctataagca tattagtttt attgcttaaa ttaagacctt tagtattaga
     2641 tataccatta cttgatacag aataagatat agtacattta gataaattta ccaccttatc
     2701 tgaataagct ttagtctctc tttccatttc taaagctaaa tcaatcaact tatctgcctc
     2761 taaattttct agctctttag aataagtctt tacatcatta tagtgcttat ctccttcata
     2821 tataaattgt acatcttcat tttcaatagt gtttactcca tcttttacat tttttatcaa
     2881 catatcaaca gctttatcat ctaaaatttc tgtataagaa taacctatct ttccatttac
     2941 cttacctcta aaagataaac caaaagattt atcaagattg tatttctcaa cttctccttc
     3001 atatatattt atacttaaat tttctcctgt ataataatat atttcacact cttcaaaacc
     3061 ttcacttaga gcctttttaa ataatatatc tttaaaattt ttaagttcca caattatccc
     3121 tcctatcttc cacctacagt aatttctttt acccttatca taggttggcc tacattagtt
     3181 ggtatacttc ctgatgatga gccacacatc ccctgagctt gttttacgtt attcccaacc
     3241 atatctatat tcataagtat atcgctacct tttccaatta aactagcacc tctaactggc
     3301 tcttgtattt ctccattttt tattaagtaa ccttctgaaa ctgcaaaatt aaactctcct
     3361 gttacaggat ttacagaacc accacccatt tttttagcat aaagtccatc tggtattgac
     3421 tttataatat cttctggtct atcttctcca gcagcaatat aagtgttagt catccttgaa
     3481 gttggtgcaa atttgtagtt ttgtctacga gaacttcctg taggattcat tcccattcgt
     3541 ctaccattaa gtttatctat catataagac ttcaaaattc cactttcaat cagtatattc
     3601 ttttgagttg gatttccctc atcatctata ttcatagaac cccagtagtt tggtattgta
     3661 ccatcatcta tagctgtaat ctttgtggaa gcaatctgtt gacctaattt atctgcaaat
     3721 acagagttcc cctttgcaac tgatgtagct tccaaagaat gtccacaagc ttcatgaaat
     3781 attactccac caaatccatt atctattgct accatcatat tacctgcagg acaattttta
     3841 gcatgcaaca tagtatgcgc tactctagct gattcttttg cataatattc tgggtctatt
     3901 tcttcaaata tttcaaaccc tttacaacct ccaggacctt caaatccagt ttgattttca
     3961 ttacccttag atgcaattga acttatccct agtctagttc ttattcttct atcctcaaca
     4021 tatatacctt cagtgtttgc aattaatatt ttttggtctt tatccaaata actgacactt
     4081 acttgagata tatcattact aaattctttt gcactcttat atgcaacctt cataacactt
     4141 actttatctt tatttcctat tgaatttgga taaattttta tattatgtat gttatttatt
     4201 ttaatactgt catttaatat tacacttttt ccatcttcca atttacctaa agcaacagca
     4261 gctttgtatg ctacatccaa cagactattt aatgaattgt cacttgtata agcatatata
     4321 cttttcaatc ctttaaatat tcttattcct ataccataat ttcttccacc tattgcattt
     4381 tctactttat tatctattaa actaatagaa ttatttatag tgtcttcttc aaaaatctca
     4441 gcaaaatctc ctccagtaat tagacatttt tctaatacct tagaagctat attttttgaa
     4501 agcataaatt tacctcctac tttaaatttt taattaactt ttattcaatc tttttacttg
     4561 ttaacaaaca ttatatacta tattacttaa aataaggtaa ttatgaatat attatacata
     4621 tgttttatac taaaatgtta tcacatttta tctatacata cccatataac tagataatta
     4681 aaatgtataa tattatttgc tatagaaaat aatacactca tacataataa attttaggag
     4741 tgaagtatat gaatcaagaa tatgtaaaag gaaatcctat ttttactaat attaataaag
     4801 ttccaagaca atttccatac cttactgatg atattgatac tgatgttata attgttggtg
     4861 gtggcgtaac tggatgtatt tgtgcttact atctagctaa aaacaatata aagtctgtca
     4921 ttcttgaaaa aggaaggatt gcacatggaa gcactagcgt gacaacttct ctattacagt
     4981 atgaattaga tgacaatttg atagatttaa cagaggttat gactctaaaa gatgctctaa
     5041 aagcatacaa cctttgtact tcagcacttg aagaattaga tacatttata gagctatatg
     5101 gaaataagtg tgattatgcc aaaagagata ctctacttta tactgccaac aaacttgaag
     5161 ttaaggctat caaagaagaa tataacttaa gaaaagaaaa tggatttgat gttgaatata
     5221 ttgatgaatc cacaaaccct tttagctttg atttaaaatc aggattgata gcaaagaatg
     5281 gaggacgaga attagaccca tataaatata gtcaccattt gattgatgta agtttaaaaa
     5341 atggtcttca agtatatgaa aatacagaag taaaaaaagt agatttatca aatgataagg
     5401 ttactgctga agtatcgtat ggatacaaag ttcatggtaa aaaattaatt gtagctacag
     5461 ggtacaatac aagtttattt acaaaaagaa attttgcaac taagtcaaat acttttaata
     5521 tagcaacgaa accacttaaa aacatagctt cttggaaaaa caacatttta attagagata
     5581 actgtgaccc atataattat ctaagaacta ctaaagacaa tcgattaatt ataggtggtg
     5641 aagatgtaag ttttgatgat atagaaaatg aaactttagc caatgaaaag tatgatattt
     5701 tagaacaaag attgaaaagt atgtttaagg atataaaaga cattgaagtt gaatataaat
     5761 attgtggttg ttttgcttca actttagata atttaggttt tattggtcca gataataaaa
     5821 ataacaatct atggtattgc ttaggttatg gagcaaatgg tatactcttt gcaatattag
     5881 gaggtataat gttaagtgaa ttatatcttg gcaaacaaaa taaaaatatg aaattattta
     5941 aagtagatag atttgataaa tagatattat atattaaaag actgtaaaaa gatgaaactt
     6001 tataattcta aaagttaact ttgatacagt cttttataat gttcctttta aaaatacttt
     6061 atttataatt aaatataaag taaaatgtta aaatataact aataaaattt agtcataaga
     6121 taatatttaa gtttatttga tgaaatatgg aggaattatt atgaaggaaa tactcgttat
     6181 tgctcccact aaaggaacct atgaaaaatc aattcacata gttaaaaaaa acaaatatac
     6241 aaatattgat gttgtatttg gcaatctaaa agaaggtata cctcttgctg aaaaaagtat
     6301 taatcatggt actagaatta tcataagccg aggtggaact tacaatatgt taaaagctac
     6361 ttacaatata cctatagttg aaattaaagt agatgcttat gatattataa aaagctacaa
     6421 ggaggtaaaa aattccaacg aaccatttgg aataattgga tttaataatg tcatctacgg
     6481 tttcgatatt atagaagaaa tattgaataa gaaaataact atgattgaaa ttgaaaaaga
     6541 agaagaaatt tatgatgcta tagaaaagta cagaaagaaa ggtataaaca catatatagg
     6601 agataccact gttgcacata ttgttaagag acttaattgt aagggaatat taatcgaatc
     6661 tagagaagaa aacatactta gagccattca acaagcagaa caaatactcg aagcaaccaa
     6721 ggatgaacaa aaaagaagat tacagataga agctatgaca gattttgtac atgatggaat
     6781 aataacagtt gataaaaatt ttaaaataac attatttaat aagagtgctg aaaaaatatt
     6841 tggtataaaa aaagaaaatg cactttatca taatgttgta gatgttatcc caaacacagt
     6901 tcttccacaa gtagttgaga ctatggaacc tcaaattggt gaaatacaaa atatagggag
     6961 tacaaaaatt attacaaata gagttccaat aattgttgat ggtcagattc aaggggctgt
     7021 ttcaacattt caagatagca aggagataag ttcttttgaa catacaataa gaaggagctt
     7081 attaagcaaa ggtctatctg ctaaatacac ttttgatgat ataattcatg ctagtgaaaa
     7141 gacaaaagat tgtatagaac ttgcaaaaaa atatgcttgt tatgatactc ctatgattat
     7201 tactggagaa tctggagttg gtaaagaact attttgtcaa agtatacata actatagtaa
     7261 gcgtaaaaat gcaccttttg ttgctgtaaa ttgtgctgcc atacctccat ctctaataga
     7321 gagtgagttt tttggttatg aggaaggttc atttacaggt gctagaaaaa aaggtaaagc
     7381 aggtgtattc gaactagcac atgaaggcac aatcttccta gatgaaataa gtgaaatccc
     7441 tcttgaactt caaggtcgat tattgagggt attgcaggaa aaacaagtta tgagaattgg
     7501 tggtgataaa gttattccta tagatgtaaa aattatctgt gcttcaaata aagacttaaa
     7561 aaatatgatg cgcaatggtt tatttagaca agacctttat tttagaataa atatactaac
     7621 attatatctt ccttcattaa aagaaagaaa agaagatatt ataaaattat cagaattttt
     7681 tatacataaa tattcaagca aatacaataa aacgccttta attatcactc caaatattaa
     7741 aaaggcactt cttgaacgag aatatgaagg taatataagg gaattggaag gtatgatgga
     7801 aagggctgtt attgtggaat catttgactc gattttatta gaaaagctta gcaatgaata
     7861 tacaaataat atagttgata aagataatag tgattttgaa tgcagtaatt tagatttaaa
     7921 aactttagaa aaaaaatata tctataaaat ttataacgaa aataatagaa atactacaaa
     7981 aacctgtgaa attctaaaga taactcgctc cactctatgg cgtaagttaa aagaaataga
     8041 agatgatgtt taaaaataat acatatctat atatatcaaa acgttttatg tttaatttta
     8101 atacataaga cgtttatttg ttttcaataa aaaatacaat tatatgctaa aaagaatctt
     8161 tgtaagtgaa attctcgtca atcatacttt taattataag taatctacag catttcaatc
     8221 gattattaaa aataatcatg ataactaaag atacaaactc tatatattgg catagtaatt
     8281 gcttttataa tacctatata aaaatgaaag ggagtgatac tagatgcaca tcttaaaatc
     8341 agtgaaaaag ataccaggag gacttatggt ctgtcccttg ttattgggag cactatttaa
     8401 tacagtatgt ccacaagctc ttgaaattgg aggattcaca acatccttat ttaaaactgg
     8461 tgcaatgagt atacttgctc tattttgtct atgcaatgga gctcaaatca atatcagaca
     8521 agctggtaaa ccattgacta aaggtatagt acttacagca acaaaattta taattggtgc
     8581 agtattaggt attattgtta gtaaatttat tggacctaaa ggtataattg gtattacacc
     8641 tcttgccata gttgctacca tgactaattc aaatggaggt ctatttgctg ctcttgcagg
     8701 tgaatatgga gactccacag atgttggagc aatatctatc ttatctctaa atgatggtcc
     8761 tttttttaca atgcttgctt ttggagtaac tggacttgca aatgtaccta tagtagcact
     8821 atttgcagca cttataccaa tactacttgg atttatactt ggtaaccttg atgaagatat
     8881 acgagaattt cttgcacctg gtacaacatt attaattcct ttcttcgctt ttcctttagg
     8941 agcagcatta aactttaatc aaattataac tgctggacta cctggagttg tacttggttt
     9001 aggagttact ataataacag gtatgggtgg gtactttgta atgaaactta tacgtgcaga
     9061 acatccagca gtaggtgcag ctacaggtac aacagccgga aatgcagctg gtactcctga
     9121 agctttagca gctatagacc ctactctagc tacaatagca gctgtttcaa cagtacaagt
     9181 tgctgctgct attattgtta cagcaatatg ttgtccaatg ttagtttcct atctagataa
     9241 aagagaaaaa aagaaaaaat ctatttttaa aacaaataaa aatattgaag taaattaata
     9301 cttaaggagg taaaattaat gaatgcaatt attcatgcaa atgaaaaaga taatctagtt
     9361 acatgtgtca gacctttaat taaaggtgaa aaagtttcaa ttgatggaaa gtcttacata
     9421 gtaaatgata atatacctgt ctttcataag atggctacag aagatgtaaa aaaaggtgat
     9481 gttgtataca aatacggtga aattatagga attgcaacag ttgacataaa aactggtgac
     9541 tatgttcatg tacataacat tgaaagtaca cgtggtcgtg gagataaaaa ataggaggaa
     9601 gttaaaatga aattaatggg ttatttaaga gaaaatggtc agtttggtat tcgtaatcat
     9661 gttttaatta ttccaacatc tgtatgttca agtgagacag ctacaagaat agcatcactt
     9721 gtgcctggag caatagcaat ccctcatcaa catggatgtt gtcaaattgg ttctgatata
     9781 gagcttacag caaagacatt aataggattt ggaaaaaatc ctaatgttgc cgcagtttta
     9841 gtagttggtc ttggatgtga tggtattcaa gcaaaagaat tagcatctga aatagctaca
     9901 acaggaaaaa aggtaaatta cgttgttata caagaatgtg gtggaacatt aaaaacagtt
     9961 tctaagggag ctgaaatagt aagtaaaatg gctcgtgaag tatcaaaaga agtaagagtt
    10021 gaatttggta tgagtgaaat tacattagca cttgaatgtg gtggttctga cccaacaagt
    10081 ggaatagctt caaacccttc aataggcgtt gcatcaaacc ttcttgtaga tgaaggtgga
    10141 agtagcatac taagtgaaac tacagaagta attggtgcag aacatttgtt ggctacccgt
    10201 tttgaagatg aagaaatgaa agataagttt cttaaatttg taagtgatgt agaaaaaaga
    10261 gcaattgcta tgggagaaga tttacgtagt ggtcaaccta ctccaggcaa taaagctggt
    10321 ggactttcaa caattgaaga aaagtcactt gggtgtatgt ataaggcagg caataagcca
    10381 ttcaaaggtg ctcttgaata tgctgatatt gtacctcctg ataaaaaagg attatatttt
    10441 atggatactc caggtcaaga tatagattct atcactggta tggtagcagg tggtgcacaa
    10501 attgtaatct tttcaactgg tagaggtact ccaacaggaa gccctatttc accagttatt
    10561 aaaatcactg ggaatagtga tacatacaac aaaatgccag acaatatcga tataaatgca
    10621 ggtagaatta taacagatgg tgctaaaata gcagatatag gtcaagaaat atttaatgaa
    10681 ataatagaag tctgtaatgg taaacatacc aaagctgaaa gtcttggtca tagagaattt
    10741 ggcatttaca gaatttcatc tacattttag attgtaacaa aatatctttt ataaatataa
    10801 tacttatgta gatacataag ctaaaagttc tttctaatac tataaaaatt ttctatactg
    10861 aaaaggaact gtatcaaaat tattttgaaa acagttcctt ttttaacctc ttttactcaa
    10921 aatattataa aataagattt aaacaataaa cgtatcttga ttaatctcta agaagacata
    10981 aataaagcta aatgtaaagt gggataaata aaatttaata tctttgtatg aaaagatatt
    11041 tggtataata tgtctgagag aattcttatt tgtaaacaaa taaaggatgg aggatgaaac
    11101 aaaagaatga actttgaaaa cacaaaaaat aaaaaaataa tagctacttt actattgaca
    11161 ctccttttaa tcgtgtcatt aacatgtgtc acttttatta tgaatctaaa taaagtaaca
    11221 cataatgaaa cagaacgata tcttgtagaa gtttcacaaa gtatttccac tacaattaat
    11281 gtgaaatgta aaagtatatt taggacacta tattctatgg gagatactta ttttgagatt
    11341 aatgatagtg aaaaagaagt caatagatac ttatcaaaaa aattaaaatt atttgaattt
    11401 gattggttag gtattataga ttctaatgga aattttgtaa gtgcccttaa agatacaggt
    11461 aatgttaaag gacaagactt ttatagaaaa gcccttcaag gcaaagaaac aattactcct
    11521 gttaaatcac aatctggtac atatggaatt ttatattctg taccactata caatggtgat
    11581 aaaattgttg gagctgttgc tgcttggaat actattgata ctatgagaaa tttattaagt
    11641 gtagaaagct ttcatggtga aggattttcc caaattatcg accaagatgg aaattttatt
    11701 gtaagttcag caaacaaaaa tgctccaaaa aatgtggtaa atttttttga catgattaaa
    11761 caccaaggaa aacaggttga taaaaataaa cttgaagaaa tgaaaataaa cttaaaaaag
    11821 aacaaaactg gttctatgta ttatacatta aatgatggta tttctaaggc aatgcattat
    11881 cttccattac aagaagaaaa cttatacctc ctttcaattg ttccaacaaa aacagcaagt
    11941 gcacagtttg atttacttct aaaaaaagca atcttcatca atattttaat tgttatacta
    12001 tttttattgc ttattatatt gattatgttt ataaatcaaa agaacagggc acaacttata
    12061 aaaatcgcct atgtagactc tgtaactaaa ggttacagta aagcacgttt tgatattgaa
    12121 gctaaaaaaa ttatacagtc atcttcatca gaaagctatt cattggtatc tataaatatt
    12181 cagaaattca aacttattaa tgattctttt ggtagtgaag caggagataa aacattaaag
    12241 tacatacatg atgtcattag taattatcta gaaccaggag aattgctttg tcgagtttct
    12301 aatgaccagt ttaatgtact tattaaaact attcctcaaa aagaaattct taagtacata
    12361 gaaaatatca ccaatgatat taatagtttc aataaaaatg aagaatgtaa atattttata
    12421 gctctatcta taggtgtata taaaattgat gacccaaatc tacctttagt ttcaattcaa
    12481 gaccgtgcaa atgttgcacg aaaaaataat aaaggaaaca ttggaaatca tccactttat
    12541 acatgtgttt tttatagtga tttagagcgt ttaaaaatga ttaaagaaaa agaaatggaa
    12601 aatagaatgg aggaagcatt aaataataac gaatttatag tatatcttca gcctaaaata
    12661 agcctcaaag ataatactat tgtaggtgca gaagcattag taagatggca agacccaaat
    12721 aaaggtctta taccaccaga cgagtttatt cctttctttg aaaaaaatgg ttttattaca
    12781 aaactcgacc actatgtttt tgaaaaagta tgcagtatga taagaaaatg gatagataat
    12841 ggagaaactc caatacctgt ttcagttaat ttttcacgtg ctaatttaaa taatatagat
    12901 tttttagaaa aatacagaat gcttcgtaat aaatacaaca ttcctgctaa gctcttagaa
    12961 attgaattaa ctgaaacact tgtatttgaa aatctccaaa aattaattaa tataatagac
    13021 caaatccatg aagaaggttt tcaatgttca ctagatgact ttggaagtgg atactcttca
    13081 ctcaatatgt taaaagaaat aaaagttgac acattaaaac ttgaccgtgc tttttttagc
    13141 tcacctaatg cagataatat tgctgaaaat catgtaatag aatctatagt taatttggca
    13201 aaaaaattaa atatgaatag tatatcagaa ggtgttgaaa caatcttgca aatggattat
    13261 cttaaaaaaa ttaattgtga tatgatacaa ggatatgttt tttcaaaacc tctacctcaa
    13321 gaaatatttg agaaaatgac ttttggaaaa aaactagaag aaaccagtat ggatagttcc
    13381 gtttaaataa aataaaaaac ttgtctctat aaaataattt taatcatttt ataaagacaa
    13441 gtctttttat tttccaattt aattttattt tctaaatttc ttacactcgg cttgttttaa
    13501 attccctttt taagaataat aatacaatta tcatagcaat aaaatctgca actggctgtg
    13561 ccaaccaaac accatctaaa ttaaattgaa ttggaagtac aatgattaat ggtataaata
    13621 aaataaattg tcttaaaaga gttaaaaaca ttgaatgttt aactttactt atacactgaa
    13681 aatacacagg tcctacaata gaaatcccca ttataggcaa tgtaattaga tttaggctta
    13741 tacctcttac tgctatatcc atcaaatctg aatctttatt aaatatacct acaaatactt
    13801 ctgggaaaac ttgtactact aaaaatccaa atgacaatat gacaactgac actatgatag
    13861 ataatattaa agttttcttt gcacgctcat attgttttgc accataattg tatgatatta
    13921 tagtttgcat atcttgattc aatccaaaca caggcatcat aaacatcaat aaaatagatg
    13981 taagtgctgt catagctcca atagctaaat ctccaccata aacctttaga gaattatttg
    14041 taaacatgtg agttatactt atagctactt ccatagcaaa aggtgtcatt cctattgcaa
    14101 ataccttctt tactatatct ttattcaatt ttaagttaca tttttttagt tttaaattgg
    14161 acttccctct tataaaataa tgtattaccc aaaagaatac tactatttga cagagtactg
    14221 ttgctatagc tgctcctttt atacctaaat taaatacaaa tataaatata gggtccaata
    14281 tcaggtttaa tacacatcct acaatcattg tccttgctgc taattttgga ttaccttctg
    14341 ccctaattgc attattaaga gaaaatgcaa ctaaattaaa tatacttcca accaatatga
    14401 cactcatata gtcttttgca taaaatattg aatctttact tgcacccaaa ataaaaagta
    14461 tatcttctaa gaaaactaat cctaatatag ttataattaa agagataatt atagatagtg
    14521 tcaaagcatt acctaatatc ttctctgctt cttctctatt tctttcccct aactttatag
    14581 acaatcttgt gctagcacct actgatacca acattccaaa agctattatt agagtaaata
    14641 ctggcatagt tacaccaaga cctgcaatag ctatagagcc aataccttcc atagaaccaa
    14701 taaaagctct atctactaca ttataaagtg aagttaccat cattgctatt attgcaggta
    14761 tcgaatacct aaataataaa atccatattt tttcatgtct taatgcttct tgattctcca
    14821 tgttgtcacc atttacacca tgtcataagt tatacaaatt ggtttatttg ttcttgggtc
    14881 tgttgctatt tctgcatcaa tattaaataa ttcttttaga ttatccttcg tcataacatc
    14941 atatgcatca ccttcacaaa ctattttacc tttcttaata cctatcatgt ggtcagcaaa
    15001 tcttgctgca ttattaagtt catgtattac catgacaata gttcttcctt gttttttatt
    15061 tagttcatct aataatttta gtatctccaa ttgatgtgcc aaatctaaat atgttgtagg
    15121 ctcatccagt aataatatat cagtttcttg tgccaaagcc atagctatcc atgccctttg
    15181 tctttgtcct cctgataaat cttccatatt tctatcctta aattctgcaa ttccagtaac
    15241 ttctaaactc caatttacaa tctctctgtc ttctttacta gaattaccaa atccactttt
    15301 atgaggaaat cttccataag aaattaattc tgaaacggta agaccactag gagcttgagg
    15361 gctttgtgga agtacagcca tatcttttgc tatttcttta gacttctttt tgtagatgtc
    15421 ctctccattt atatatattt tcccactatt agggctaata attctaccta tagttttaag
    15481 tattgtcgat tttccacaac cattagcccc tatgatggta gttatttttc cttttggtat
    15541 atttaaattt aaatttttta taatatctgt gtttccatat gatacattta gattcttagt
    15601 atttatacag ttcataacaa caccctttcc taatcattta ccaacattaa ataaataaag
    15661 tacggcactc ctattataga aactactata cctactggta tttctatagg tgctagaaga
    15721 tttcttgata tagtatctgc tattaaaagt aataatgttc ctatcaatgc tgatactggt
    15781 attaatcttt tatgctttgg tcctattatt tttctaccta tatgtggagc tattaaacct
    15841 aaaaatgaaa tactacctgc tactgctgtc gatacaccag ataaaattac tgcatatatt
    15901 ataagtcttc ttctttgttt ttctacatct acaccaagac ttatagatac ttcatcaccc
    15961 aaatttaaaa catctatata tttagattta taaatagtta tacccataaa aatcactata
    16021 aatggaagca cagctattac atatttccaa ctggttcccc aaatgcttcc tgatgtccat
    16081 gccataaccc tattaaattc ctgtgttgta aatttcaatt ggaatattgt caaaattgaa
    16141 gtaaaagcta tgtttacacc tataccaata agtagtaatc ttgatgaatt tataccacct
    16201 ttccatgcaa gagaatatat aagaaatgct ccaaataaag ctcctataag tgcaacaata
    16261 ggcattgtaa atacagtcaa gttactcata ccttcatata cattaccatt cataaggtat
    16321 atataaacta ctacaaacaa tgcagcacct gaatttattc ccaaaattcc agaatcagct
    16381 aaatcattct ttgtaactcc ttgaagtatt acacctgctg ttgataatgc agaaccaacc
    16441 aaaagtgcta ttactattct tggtaatctt aatttaaata ttgcaatttc atgattacgc
    16501 tgaccctgcc ctaaaaaagt ctttataaca tccattggtt ctatagaaaa tgagcccata
    16561 tttaaactca ataaaaaagt gataaaaata agcaccatta aaattgatat aacaagtaat
    16621 gttttagaat ttatttttat aatatttttc atagtaccta tttctccccc tttctaacaa
    16681 ggtatataaa tattggcact cctataagtg ctgttataga acctactggt gtctcatatg
    16741 gaggatttat catcctactt aatatatctg tatatacaag caatacagct cctaatacta
    16801 aagagctcgg tattatatac ttataatctg aacctactat tcctttagct atttgaggaa
    16861 ctataagacc tataaaaata atattaccag acactgacac agatgctcca gtcattaata
    16921 ttacaagtac tatacatata aatctaacaa agttagtttt ttgtcctaat cctattgcca
    16981 cttcttctcc aagacttaat atagttattc ttggagccat aataatagct aaaataaatc
    17041 caataccacc agctacaaat agcaatttta taccttccca cttcactcct acaagccctc
    17101 ctgatatcca gaaacttaat tgttgagata ggttaaaata catagatatt gctgatgcca
    17161 aagaaattag caatgtacca agagcgactc ctgcaagagc taattttaca ttattaactc
    17221 ttcctcttga tttagaactt ataaaatata taaataatcc acttatactt gctccaataa
    17281 atgcaaacat cattacacta aaacttccat gtataagttg aggaagtttt ttttgaagta
    17341 ccagtgatat cgaaattgcg aatgtagctc cttgtgtaat tcctataaca gatggttcag
    17401 ctataggatt tctcgttatt ccttgcatta ttgccccaga aactgctaaa aatcctccaa
    17461 caagtgcagc tgctattgcc cttggaagtc taacatcctt tactattctc atatttattc
    17521 catcttccat ttttatcaaa ctatctataa cagttgatag attcatatct tttgccccaa
    17581 tagttatgga taatacaatt ccaaatacaa gtacaactat acttactaat atcaacaaat
    17641 aagttatatt ttttttactc agcttagtca attatcctac ctccttttcc cttttaatcg
    17701 tcagtataat atcatctatt aagtatttta attttaagat ttattaaaaa taggtgtgaa
    17761 aaatgaatat cattatctca cacctactaa gttaagaatt actttgttaa ttcattttta
    17821 actgattcta ataataactc ttttccaatt ggattgtatg attgactgaa aaatggagca
    17881 gcatcaagta ttgttacatt accatctttt actgctctta tttgagccca gatagcacta
    17941 ttttctaaat cttttttatc tgcttctgta gcaattacaa ctatatggtc tgcatctata
    18001 tctgttaaac cttccatact taccattggt agtgttattc catcctgttt tggcatattc
    18061 ttaggtttag ctaatttcat atcatcattg attagagtac ctattccacc atcactaaat
    18121 accataaatt gacctgaact tgccaacact ggtaaataag ttttttctcc attcttttct
    18181 ataacttcct tacctaactt tgtagctttt tcatcatact tctgtagcca acttttagct
    18241 tcttcttctt tgtcaaataa ctttgaaaca tcagttaact tgcttctcca gtcatttgca
    18301 taatctttca tcataactac aggagctatt tcttttaatt ggtcatatat tttttcttgt
    18361 ctttgactca ttattattaa atcaggattt acttctaata tagcttccat atccatagta
    18421 tccatcattg agtgcccaac tatctttaca cctttaagct cttctcttat gtaacttggt
    18481 aatttatcag tttcatatga gtctacattt gctgttgcaa caggcttata acctgctaat
    18541 aatagttctt cactacttcc tgatatatca actatttttt taggatttga tggtatctta
    18601 acctctcctt taacagattg tacaactctt gtctctgatt tatctttatc ctctgtattc
    18661 ttatctgaac atgctgtaac aagtactaac gtagttataa tagatataaa tattgcaagt
    18721 gattttattt ttttcatgat tacttctcct acttgtttat tatacttaca tgatattgat
    18781 tatcataagc acatatttat tatagcattt ttctattttt agtcaatatt attttgtaaa
    18841 aggataaaaa ttcctaatta atcttaatta tatatagctt agataaggtt ttcttatatt
    18901 aaacagtatt tttttaaaga attggacaca aatacaatga cttttcagtt aaacaatgat
    18961 aaaatgcaat ttatagataa tgaatttagt aaggaggatt caaaacatgg tacatttagt
    19021 atattgtgat aatgcaggaa aaaaaggaga aaaggtctta gataaaatct tggctggaac
    19081 aaaaacaatg gttattagag gtgctgctgg tagaaaaata ccacatagta gagtatttga
    19141 agatgagtta ctttatttta tggaaaaagg aactgcaaaa atatctgcaa aagccactgt
    19201 taaaaaggta gaaaactatg ttaaattaac agaagatgaa atttctaaaa ttttaaatga
    19261 aaatcaacca aaattaaatt tatctgaaaa gcaaaaagtt cgttggcata aaaaatgttt
    19321 atgcttagtt gaatttgaaa atgttgaaga aataacacct cttgattttg accatcaagg
    19381 aaatatggat gattggctta ttgttgaaaa aattgaagat gtagttgttg gtactagtat
    19441 accttacaat tatgagaagt ctaaatttta aagtatcaat aacataatac tatattgcta
    19501 aactctactt aaataaaaaa agagtgcatt taataattat aaaaattatt tttatacact
    19561 cttttttaat aataatttta aattacttac tttctaatct ttcatataca tctataaatt
    19621 cttctgctaa ttgttgaaaa ttcatagctg tcattaaatg gtcttgtgca tgaataagta
    19681 taacacttat ttcagtttta ttaccattag cttcattttg tatcaattct gtttggaatt
    19741 tatgagcttt atgaattgat tctttcgact tttcaataca ttctctagct ttatctatat
    19801 tcccgctctt tgcttctttt attgcttcaa atgctagccc tttcgcatca cctgcaaatc
    19861 caattattcc aaaagatatc tctatcatct tctcatccat ttattcgacc tcctaaatat
    19921 ctctatagct tgatatgact aatccattct tttctaaaaa ttctttgact tttttagatg
    19981 taagtatctt gtgctctttc gttctatcaa tagcataaga tgtagaattt aaaatataat
    20041 catctaaaaa agctgggtga gacataatat ccactacatc atacttcatt atttcgtcta
    20101 tattttttat aaaatattct tcacttacat tttccttata aaaactatct attaaaggaa
    20161 ctactttaga atactctaaa ttatattcaa atccacctct tattggaaga ttatacttgg
    20221 ttacaatctt ctctattact ggctttaaac ttaataaagt atggatatga tggtgactat
    20281 ctaaatgaga tatatctatt ccaagccctt ttaccctatc tatttgagca caaagttctc
    20341 tataaatctc atcacaatcc attttttcaa taatttcgtc tgtaatcctc ctaataaaaa
    20401 aaccgtcttt atcaacaata gtttttaagt tagataataa tggtctacca caacttaaag
    20461 tcatatgaac tccacaattt aaagttttat tttctttaag taagcctact ccatgttcta
    20521 ccccaggcat atttgccatc atagaagtag acctaactat accattatta tgagctgaaa
    20581 ttattccata gttcacagct tcacaatatc caaaatcatc tgcatttatt ataattttag
    20641 tcataccata acctactttc tatagacaac tatctattaa atctaggaag ataatcttta
    20701 tgagcttcca acatttcgtc taatatagtt tttgctaagt catcagatgg aatcaatggg
    20761 tttatacaaa gtgccaaaag agcagattca taatcgccat atacagctgc tttagctgcc
    20821 aatacttcaa atgatttgat ttgacttact aatccatgca cagaatctgg taaatatcct
    20881 atacttattg gttttggacc attttttgtt attacactac ttacttctac cgcttgttca
    20941 tctttaaaat ctcttataga acccttattt aatgtattaa ctacttggat atcttttttg
    21001 tcgttgtata tagaacttat taaattacaa gcagcatcac tatagtaagc ccctcctctt
    21061 ttttcaagtt gaggtggttt tacatctaat ttttcatcct tatataattc aaataattgt
    21121 tcttccaatt ctttaactac ttgacctcta gcttttccct ctttaaactc tctcaactca
    21181 tcctcaagca tttctttagt tttatagtag tatctatgat atggacaagg tattgcaccc
    21241 aaagatttta caaattcagc attaaaatca attgccttta tattttgcat acttatatct
    21301 gctttgacaa atttatcaat tgcttctttt gtaacatctt ctccatctaa agatacattt
    21361 agaccataaa ccatatgatt aagacctgca aaatccatac ttattctgtc actttctaca
    21421 ttaaatagtt tggcaacatc attttttaaa tgaattggca cgttgcatag acctatatat
    21481 cttctaaaat tagtatattt gaatacagcc tctgttatta ttcctgttgg atttgtaaaa
    21541 ttcaccatcc atgcatttgg acataattct tccacatctt ttattatatc aaatataact
    21601 ggtattgtgc gaagggcttt aaataaacct ccagcaccat tagtttcctg ccccataact
    21661 ccatgagata aaggaatagt ttcatctttt attctcgcat ctaaaagacc tactctaagt
    21721 tgtgttgtca caaagtctgc atctcttaga gcttctcttc tatctaaagt aagatttatt
    21781 ttcatatcta ctccagcttt ttttaccatc ctcttagcta gatttccaac tatctctaat
    21841 ttttcttttc cagcttctat atctacaagc cataattcct ttacaggtaa ctcttcatat
    21901 ctatttataa aaccctctac aagctcagga gtataacttg aacctcctcc aattgtaact
    21961 atttttagtc cctttttcat ctcattatac catccttacc tataatttaa tatactctac
    22021 aaaaccccat acatttattt aaaactctat acatttattt accgaaataa aactacctag
    22081 ttgttaattt tcaatttatt agcttccatt ttttcagacg ctatgacaaa tggaatatat
    22141 attaatattg ataatattaa attaaatagt gctaaagctc caccagtcca gtcacctgta
    22201 gccattgctc cacctaatat aggaggtgtt acccaaggta ttttaggtaa aattactggt
    22261 gctactaatc caaaatctat tgctaagtat gatacagcac tacatattac tggtgcaagt
    22321 acaaatggaa tcataaatat aggatttaaa actattggaa gtccaaatat caatggttct
    22381 cctatgttaa atattgcagg tggaccacct aatactatca tctctttacg tcttcttcca
    22441 gctattaata aagctactat taatcccaat accattccag aaccacctaa atatacaaat
    22501 gcatcaaaga atgcacctgc aagtacatgg tatccttccg ttgctcctgc ttgagccaag
    22561 gcagcatttt ctccacctaa tttcataagt atagtttctg taaaaggaag tgctatattt
    22621 gcaccatgaa gtccaacagt ccaaagtata tgaattataa atgcaataag cattgtagaa
    22681 cctagactat cagctacatt tgatagtggt gctcctaaat aagtatttaa aatatctgtt
    22741 aagaaacttc cattagaaag catttttata aatattccta ttacagtaaa tatcattata
    22801 gttaacattc ctggtattaa ctttgcaaat gatcttgaaa ctgctggtgg aactccatct
    22861 ggcattttta tagttaattt ctttacctta gataatctta caaatatctc agttgataat
    22921 aatcctatta atatagaaga gaataaagct gctgtaccta agtaggtttg ttgtatcatt
    22981 ccccatcctt caactactgt agttcctccc tcaggaacta ttttaccaat ttgtggagcc
    23041 attataaaaa agatagataa agctataagc ccagattgta acccatcact ctcataagat
    23101 tttgctagaa aataagcaac acctattact aagaatactg ccattgtacc tattgctccc
    23161 caccaaaggt cgcctccaaa tgtggtccag ttttctccaa atatacttgc catcaaattt
    23221 ttgtatgctt ccaatggtaa gttatttatc aatgttccca atgcacctac catagttata
    23281 ggtatcatta ctataaatgc atctcttact gctactaagt gcctttgagc tcctatttta
    23341 gcagctacag gcactatata tttatccata aatgacatga atttttccat tttacatcct
    23401 ccagtacaac tttattttga actatttaac tctacagctc tatctaacac agcttttcca
    23461 ttacatcttc cataatccat catatttata acttctactg gaatattata tggttttgcc
    23521 atttcagttg cttttcctaa tacatatcta acttgaggcc ctaaaagtaa gacatcacaa
    23581 ttttctattt cattttttaa gttcacttct gatatagccc atatttttgc ttctataccg
    23641 ttttcctttg ctgcatcctc catttttgta actaataaac tagttgacat tcctgctgaa
    23701 caagctaaca ttatttttat cattttttcc cctccaaatt agattcatta aatatttttt
    23761 aagtcataag ttttttaaac tatacattta aaatatgttt ttctattaat tttttcttaa
    23821 tgtatatatc ttatttttaa atgtatatac atttaattct tgatattatt atataccaaa
    23881 tgtatataca tttcaagaaa actttttcat tttatttaaa ttttattcga caacagcatt
    23941 caaagttaat gtatatccat agtgatatgt gtgtgagtac tcaaaaatct cactattttg
    24001 taacttacaa atttggctta tatataaaag tggctctggt tcttctaatt ttagattatc
    24061 catacactct tcattcgata ttacagcttg tacttctctc tctgacatgg ctatcttgta
    24121 tccacacact tcttcaacaa aattataaag agaattttca caatgagtct tatttaaatt
    24181 tggaaataac ttaactggca tataagtaat catatatgaa actcttacat cattagctaa
    24241 tcttactctt tttatttccc aaacattttc ttctaaactt atattcaact tacttgataa
    24301 attttcatct gcctgaacta tttcaagaga taccaatata ttagatactt tatagccttt
    24361 actttccatc tctttagtaa atccacttac agacgatata ttatcatata tattatttgc
    24421 aataactata ctacctacac ctctttgtct agttatatag ccttctttaa ctagattatt
    24481 aagtgcagac cttacagtca ttctagttac attaaattct tcactcagct gtttttcaga
    24541 agggattaaa tccccttctt tcaaactgtc tgaatttatt aactctctaa catgattttc
    24601 tataacttta taaattggct ctttcattta attcactcct tattaaatct tttatataat
    24661 aagataattt tatatacaaa caaagaaaat gtctatacat ttaagataat ttaaatttta
    24721 ttcagaaatc ttgtacaact ttccgagttt taataaagat ataaatagac ttatcattaa
    24781 tatacctttt aatatacttg ataaggttat actccaccat acaccattta gacccaacat
    24841 ttctggtcta gatataaagt aagccaatgg tactcttgca ccagtcagta tagtaattat
    24901 aatagaaggt atataggttc tccctaatcc tttgaaagct cctgttgtta ttatttctaa
    24961 acacataaaa aattgagaat agcctaatat tttaagataa actgctcctt tttctattgc
    25021 ctcactttca tctataaaaa tagaaaatat actctctcct gcaaacacta atactaaagt
    25081 agttattata ccaagtacag aagatacagt tatagctatt ttgcaacctt tatttattct
    25141 agaatacttc ttggcaccat aattttgtcc aacaaaactg cctaatgcaa ctgatagccc
    25201 ttcagccgtc atccaggaaa ttgcttctat ttgtgagcct actttttgta ctgcaacagc
    25261 cacaggcccc catgatgcta ctattactcc taatagcatt gagaatactg taaacatacc
    25321 actttgtatt gctataggta atcccaattt atacagaact ttataatatt tagtctctat
    25381 ttctttaaat agtcttaatt taaaatattc ttctttactt ttaactatta tatatataaa
    25441 acatgtagta actactatct gagcaaatac agttgcaata gcagctcctg caacaccaag
    25501 ttttggagca aatccccatc caaaaattaa tatagggtca agtataatat tagttattaa
    25561 tcctactgta ttaatacgaa aaggagtttt actatttcca agaccattga aaattgcagt
    25621 aaatactgga ttaataaaat aaaataccat acctagagct actattatca aatactgtct
    25681 agacatggtt ataacttcta agtctcctaa tctaaaaaaa cctatcaatt gtttattaag
    25741 tactattaaa gaaattgtat atattattgc tagtataata tttatttcta ttgctgactt
    25801 catatatgat ttagtagtgc ttatattatt tgcaccaata ctttgtgcta cttttatctc
    25861 tccacctact tttgaaatca taataaatgc cattgcaagc catggaaaaa aaccagctgt
    25921 tccaactgca gctactgctt tacttccggc tttaccaacc catatcatat ctatcatgtt
    25981 ataccccatc tgtataaatg aagtccccat tattggaagt gatagcttaa gtaatttttc
    26041 tgtaattctt ccttctgtta aattaagtct gtctttcatt ttccacctct tttatattaa
    26101 atttatttgc ttatatagct agattaatca ttttatttga ttgagttcac tatccttcaa
    26161 tttatattat catatctcag gattgcatct caatcatact tatgtttaag atacagtatt
    26221 ttacctatct atgataaaat taatacttat gaatataaat ctagccattt catcaataca
    26281 tattctaacc tttaacttaa cttcaattaa gtatacatat cgtaaccaat tagaatattt
    26341 tatacggtat cttccattaa taaacaacca atatcaatga tttatatata attttattta
    26401 tacataaaat agtatactat aataataaaa aaactatatt tgaatgcttt aagcatgttt
    26461 actaattgta atttccacac aaatttgata atatatgaat atattatggt gaaatttata
    26521 gatatatatt aattatgggg agattgtggg aggttctttt cacaagacat ttaagctatg
    26581 aaaataaata aaaaattcaa tagagttgat atgcaggttt caatacttac tgcaattatt
    26641 gtaataatat cttcctcatg tgtatatttt tttaattata cactaactta taagagcatg
    26701 atatactctt taagtgagag gtcaaaacat atctatgaat atgtagaaag aaatattgat
    26761 aaaaatacct ttactgaaat taattctaaa gaagaccaat caaaaaagtc ttataaaagt
    26821 tctaaaatcc ttttagaatc tgtaaaaaat actactggag tcatgtatct atatactgct
    26881 aaaaaaacaa aagatggatc actagtatat gttgttgatg gtttaaattc atctaggagt
    26941 gattttagaa atgctggtga tttgatagaa aaagagattt ggggagacct taacaaagca
    27001 ttagataatc atatattgtt acctaaaaag attaaatcaa catcttgggg atacatattt
    27061 atatcgtatt ttcctattca caatgatgtt ggtaaggttg ttggagtaat aggaattgaa
    27121 tttgaagcaa aacatcaata caatacattt aaatttataa gtatagttac accaataata
    27181 gcattattaa cttgtctaat atcagcattg attgcagtga ttctatttaa aagaatatct
    27241 aatcctacat atactgattt tgctaataca gattatttaa caggcctaaa aaatagaaat
    27301 gcttttgaaa tagatatgaa taacttaaac aattcaaatc taaagaatct aatatctatc
    27361 atatcaatag atttagacgg tttaaaaaag attaatgata aatttggcca tcagacagga
    27421 gacttatata ttaagcaagc aagtaaaatt atacaatatg taataaataa aaaatatata
    27481 gtttatcgta ttggaggtga tgaatacata atta
//
LOCUS       DAYASV010000042        27219 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYASV010000042 DAYASV010000000
VERSION     DAYASV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.042"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(105..959)
                     /locus_tag="ACGI68_001755"
     CDS             complement(105..959)
                     /locus_tag="ACGI68_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HFH0138197.1"
                     /translation="MKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKEVP
                     SKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDIEE
                     IEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCDKI
                     IKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLNTI
                     KEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEEEE
                     EKYRRIFK"
     gene            complement(1282..2610)
                     /locus_tag="ACGI68_001756"
     CDS             complement(1282..2610)
                     /locus_tag="ACGI68_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0138198.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2758..3087)
                     /locus_tag="ACGI68_001757"
     CDS             complement(2758..3087)
                     /locus_tag="ACGI68_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0138199.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3111..4400)
                     /locus_tag="ACGI68_001758"
     CDS             complement(3111..4400)
                     /locus_tag="ACGI68_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0138200.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4416..4742)
                     /locus_tag="ACGI68_001759"
     CDS             complement(4416..4742)
                     /locus_tag="ACGI68_001759"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138201.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4877..5506)
                     /locus_tag="ACGI68_001760"
     CDS             complement(4877..5506)
                     /locus_tag="ACGI68_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138202.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5721..8378)
                     /locus_tag="ACGI68_001761"
     CDS             complement(5721..8378)
                     /locus_tag="ACGI68_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138203.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8737..9681)
                     /locus_tag="ACGI68_001762"
     CDS             complement(8737..9681)
                     /locus_tag="ACGI68_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138204.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIDVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9674..10699)
                     /locus_tag="ACGI68_001763"
     CDS             complement(9674..10699)
                     /locus_tag="ACGI68_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138205.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10774..12342)
                     /locus_tag="ACGI68_001764"
     CDS             complement(10774..12342)
                     /locus_tag="ACGI68_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0138206.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(12455..13366)
                     /locus_tag="ACGI68_001765"
     CDS             complement(12455..13366)
                     /locus_tag="ACGI68_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138207.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13350..14336)
                     /locus_tag="ACGI68_001766"
     CDS             complement(13350..14336)
                     /locus_tag="ACGI68_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138208.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14654..15097
                     /locus_tag="ACGI68_001767"
     CDS             14654..15097
                     /locus_tag="ACGI68_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0138209.1"
                     /translation="MILLKSNYDCFRIAMLLKELYSKTMYTVEENFKENGLTHQQIIV
                     IKLIAHNQELTISQLCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTT
                     GFEFATNFKIKMQESFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15100..15498
                     /locus_tag="ACGI68_001768"
     CDS             15100..15498
                     /locus_tag="ACGI68_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsmA family protein"
                     /protein_id="HFH0138210.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15948..16172)
                     /locus_tag="ACGI68_001769"
     CDS             complement(15948..16172)
                     /locus_tag="ACGI68_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HFH0138211.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGVTMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16207..17574)
                     /locus_tag="ACGI68_001770"
     CDS             complement(16207..17574)
                     /locus_tag="ACGI68_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138212.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17658..19193)
                     /locus_tag="ACGI68_001771"
     CDS             complement(17658..19193)
                     /locus_tag="ACGI68_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6179 domain-containing protein"
                     /protein_id="HFH0138213.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EVSLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGMTYK
                     IDCDVILVNLFELIMNNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19216..19650)
                     /locus_tag="ACGI68_001772"
     CDS             complement(19216..19650)
                     /locus_tag="ACGI68_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6323 family protein"
                     /protein_id="HFH0138214.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19910..20821)
                     /locus_tag="ACGI68_001773"
     CDS             complement(19910..20821)
                     /locus_tag="ACGI68_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HFH0138215.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            21117..21470
                     /locus_tag="ACGI68_001774"
     CDS             21117..21470
                     /locus_tag="ACGI68_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFH0138216.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(21689..22630)
                     /locus_tag="ACGI68_001775"
     CDS             complement(21689..22630)
                     /locus_tag="ACGI68_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HFH0138217.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(22731..23966)
                     /locus_tag="ACGI68_001776"
     CDS             complement(22731..23966)
                     /locus_tag="ACGI68_001776"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFH0138218.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            complement(24010..24864)
                     /locus_tag="ACGI68_001777"
     CDS             complement(24010..24864)
                     /locus_tag="ACGI68_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138219.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(24992..26167)
                     /locus_tag="ACGI68_001778"
     CDS             complement(24992..26167)
                     /locus_tag="ACGI68_001778"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HFH0138220.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(26266..27150)
                     /locus_tag="ACGI68_001779"
     CDS             complement(26266..27150)
                     /locus_tag="ACGI68_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFH0138221.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         8681 a         4863 c         2791 g        10884 t
ORIGIN      
        1 aaatacaaat tatattttat taatttttac tacttgtaaa ttattatctt atacaatgaa
       61 tttgtattat ataataagta tttttaggat tctgttacat aatattattt aaatattctt
      121 ctatatttct cttcttcttc ttcagtaaac acattaaata caacttttaa atttttttca
      181 ggattttctt ttagccatag tctaactcta cttacagcaa gattagctgc ctcttttttt
      241 ggataaccaa atacacctgt agaaattcca caaaaaacaa tattttttat ttcatcaatt
      301 tcttttattg tatttaaaca tgatttataa caatgaagca gttgcttttc ttgctcttta
      361 cttggctgac caccagatac aataggtcca acagtatgta ctacaaattt tgcaggtaaa
      421 caataacctc tagttatctt agcatctcca gtatattcta aatgcccttg tttttttatt
      481 attttgtcac aatcttctct aagtctagga cctgcgcatg aatgtatctc attatccacg
      541 catagatgta atggttgtaa acaccctaat aacttgttat ttgcagcatt tactattgca
      601 tctgctctta aattcgttat attacctctc catatggcaa tcccttcctc tatttcttct
      661 atatcattta catctactaa ctctctttct tcacattcaa tcaacaataa attatgtaac
      721 atattaagta tctctggagc aatttctttt ggtggatata cattcaaaag tcctctaagt
      781 atatctctag gcttaatttc atcatttgat aaattgtata ttttagaagg aacttctttt
      841 gaaaaatatg caattaaagt gtttatattt tttcttcttt gttcgtcagt tagaggcttg
      901 atagttttat caaaatcctc aaataaattt acatcatcag cataatctct ccacttcata
      961 gccatctctc cattcttgta ttttagactt aaaaataatt atgtatttat gtacatattt
     1021 atttatttct ataaacacct acataaatcc tgttaaaagt gtaaattttt tattatgcaa
     1081 cttctctggt tatttctatt gattgatagt aaattattgt acctaaattt aatccaacta
     1141 acaagtaact aaacctactt tcctaactgc aaaatacact atgtttgcca cttgtatctc
     1201 atttgtaaat aaataaagtc cctacaaata cattaactag gctatgtact aaactgtagg
     1261 aactttaaaa atatattatt cttaattaag ttttggccaa taatttttat ttgcttctat
     1321 taaatcatct agtaattctt ttgcaactgt tgcactagga actgtcttag atagcataag
     1381 tgcttgccac aatttattat aagacttctc aatccaagca tcaactacta atttttcaac
     1441 tgctaattgt tgttctatta aacctttttg gaatgttggt attttcccta ttgataatgg
     1501 ttcatacccg tcttttccaa ctatacatgg aatttcaacc atagcatcat cttgtatatt
     1561 ttctatagct ccattatttt gaactatcaa taacatacgc tcctgtgtat taaaagctag
     1621 agctgtagct aaatccacta taaactctgc atgtattcct atcttaagcc cactgccctt
     1681 agaatgtcct tgcttttcta tcaatgcaca tgaaccaaaa acttcttttt ctcttccatc
     1741 aataacttca ttggcacgag tataatttac atcagaatga tttacaacat aatctggaaa
     1801 tagatagtac tttaagtaac tacttggtat tgtagttaaa tctgttttta ctatttcttt
     1861 agtaaataaa ttagtttcaa accaactatc tttcttgtca tttttcattc cttcttcacc
     1921 atttataaaa ccatattctt ttatatgttc tactaatttt ggtaaaagtt catttccttt
     1981 tttatcttta attgaagtat accaaccaaa gtggtttaat ccaaagtatc ttatatccat
     2041 atgtttacga gatttgagtc ctgcaatcct tgctatatta tgttccatac ctattggcat
     2101 atcacaaata tttattaccc tagaattagg tctcaacctt ctaacagctt ccgctattat
     2161 tgatgctgga ttagaataat ttaacatcca acaatttgga gaatactttt ccatataatc
     2221 tatgttttca agaacacctt ctatagacct cattccataa gctattccac ctggtccaca
     2281 agtttcttga ccaactaccc catgttttag aggtatcttt tcatctagtt ctctcatttc
     2341 aagttttcca actcttatat gagctaaaca aaaatctaca tctgtataag cctctttagg
     2401 gcatgttgtt gctaaatact ctatattagg gtccttttcc tttattagta tttcacaagc
     2461 tttagctacc ttattttgtc tttcttcatc attatcatat aatttgattg ctcttaatgg
     2521 taacctatct aagttatcta gcaacattaa tatgatttct ggggtatatg tacttcctcc
     2581 tccagctatt actactgaaa atttttccat aataatatct ccttttctat ttaaaaattt
     2641 actgtattta taaataaatt tataaatact agtactttaa agtaaaaggg agttcattta
     2701 ttaaatctat tgatttaata aactatagcc aaacttcaag cactatttac aagaaattta
     2761 attcttatta attcttgggt ccatcatctc aaatatatct acaagttcag atactaaaga
     2821 atgagcaaat aatgcatttg aataatggtc ttcagagtgc acaactaaaa aatcagtccc
     2881 actatcttca ctattcatag catttgatag taattctaaa tgatgctttg atgctgtttg
     2941 tattttctct gaggctgaat ttaaaaggtg ttttgcttta ttgtagtcac ctttttttat
     3001 ttccttaaga gacatcatta catcactttt agcatctcct gcttctgata ttatactaaa
     3061 tactttttct tccatttcag gtaccatatc cttctccttc taatgacttg ttaaaaatca
     3121 aagctatcat catctaatgt tgtagctaat tctgcatttt tatttaattc ttccttctcc
     3181 attatcttaa agaatggata atatatcaag gtcataagta ttatacttgc aaatactaat
     3241 acgattgcct tccaatctaa tgtcatcaaa aatgctgcaa cccctggagg taaaaagcct
     3301 ggtggttctg caataggtaa tcctactaat cctactttta atgccataaa attaagtgct
     3361 ccaaccaata caggtccaaa tacaaatggt acaaacatat atgggttcat aactattggt
     3421 gttccaaata ttaccggctc atttattcca aatagtgatg gtaaaatcac aacttttccc
     3481 atactattaa aactcttaca cttacttctt gacataaggt acactaatcc caaagttatc
     3541 cctgaaccag ttacttgcaa gaaaccatag taaaaaccta ttgtaaacac atattttggg
     3601 tctcctcctg ctgctattaa cgttgcattt tctgccatat atgtagctgc tattggcatc
     3661 catatacttg aagttattgc tgctccatga agcccaaaga accataataa ttgagttata
     3721 aatagtatta tagttactgc aaaaacatta tctacactgc ttatagctgg tgctaagaaa
     3781 ttcattataa tagctggtaa tggttgacct gagacatttt tagttattgc tgctattgct
     3841 gtaaataaca ttatacatac tatcattgga gttaagtttc taaaactttt atttactaca
     3901 ctaggtactt cttttggaaa tctaattacg agatttttat cattcataaa cttagtaata
     3961 tcaactacta aaagtgctac taatatactt acaaataaac cttgtgcatc taaataactt
     4021 gtatctaata caccatctac tacttgtcca catacaacaa gttgtgcaac aactgctgtc
     4081 atacctattt ctacatgatt aagattatat tttttagcta gtgcctgagc tataaaaaat
     4141 gctgaatata tagataataa actcatagta aatttattag ggataagtaa tgtatccata
     4201 ttttcagata ataatgtttt tgcacttcct tcaggtaaca tattgtttaa tgccataaat
     4261 aacatactta aagaaccaac aattattatt ggggtaatgc ttatcatacc ttcttttata
     4321 gcttgaagat gcctttgttt ttcaatcttt tgtgctattg gaagaagaaa tcttcttaaa
     4381 ttattttgaa gtgcttccat ttttattccc cccaattatc tatttttata taattcgatt
     4441 gccgacttca taacagaagg agcatccata cgaccataat gcttagtatc tattaaacca
     4501 actgctattt tgtaattctt acaagcttca actacatcat cttccttaaa cctaatttgt
     4561 ggtgctacaa gtactacatc ggtatcatta gcttcaatgt atgattttaa attttcaaat
     4621 gatgttgcct ctatatttat agttttagtt tttaactttt catgctcact acaaaaactt
     4681 ttcatctttt ccacaagtac acctgttgaa gctccatttg cacatactaa taaaatattc
     4741 ataattaccc tcctacaata tctaacctct tttattttct aattttttat cagccaattc
     4801 ttactctaat tcctatcagt ttttacttta attcttatta ttttatttta attcttatta
     4861 atatttactc taattcttag tactgccaat gattaaactt gaaagccgta tctataactt
     4921 cttcttgctt atatttttct aatattttta agtatctttc tttataatca ttatctactt
     4981 ctttttctgg tattactata ctagcttcat ttaaaaagtg gaatgaatcc cttcccattt
     5041 ctaatcctct atatgtatgc ttgtcaagtg ccacgtccat aacttctgga actttaccca
     5101 ttgcaaatcc ttttataatt atatttttta ataaatcact tgatctatct ttctcacatg
     5161 cacaaagata tcttattgca tgtatgaaaa ataaaggtct gtctccatct gcatatgaat
     5221 attcctttct tacttgattt aaattattta tgattattac agctgatggg tcccccattc
     5281 ctatatcttc aactgatatt gtaagtatcc ttctccatag cttatcttct aattctggtg
     5341 atgatatata tagttcataa gcaaattcac aagcttcttc caccatattt cttcttatag
     5401 ctttttgaaa tgacgatatt atttcatctg cactaaatcc gtttcttgta gtcattcttg
     5461 cccatgggtc atttgccttt ttatctctta atattttatc ttgcatatta tttactcctt
     5521 tttatttaaa ttttaattat ttatattact catttaataa tataagcaag tattatgcca
     5581 gtttttttag acactttaaa acactgttat ttcaatcatt tttatcatca aaacttagat
     5641 actttgtcta agtatattta tatgtgtcta atattaatat tattttagac taaaaaaaat
     5701 taccaaataa gtttttatac tcattggtaa tctttttcga ttatttctgc atattctaat
     5761 attaataata tttgatagca ctctttttca ttaatttcta tattatattt tttatctaat
     5821 tgagatacta tatcttttat tttgcttagg ctcttgtgat aatcaagtgt tttttcttta
     5881 tcaagctttt caatttctct agtatttcca atcaatccat ctaccatgaa agctaaatgc
     5941 atcataagtc caattacttt atcgtatttc ttttctatat taaatactaa aaacaattca
     6001 tcagacacat attttatatc tttaaatatc ctattataat tagagctttt taaggatgtc
     6061 tttaaacctt caactacatc atcataacct atacttatct ttataatatt ttttaattta
     6121 ttgatatttt catccttatc tatatctttt ttgctaatat agtcaattcc tctaatatta
     6181 attctttgtt cattgattac tgcaacaata ttactacttt ctttaagttt cgtaatattt
     6241 aaacttaaat ctttttctgt tttatatggg acagatataa tatctattcc atctaagtac
     6301 ctactcatct tttcttctaa tatttttctt gttttattat ctacaccttc tgaaacatgt
     6361 gatgctataa taattgtttt agacagattt tctttatttt gaatttcatt agttccaaaa
     6421 tagtactttc tcgtgttagt tatagagttg tatacgtcat ttaaaggtaa tcccatattg
     6481 gccttctgaa ctgcttctaa cacagttaat gttgtcacca taggaattac tcttactcca
     6541 cacttttttt ctttctttat aatttcatca aaagagttta atgaacccat atctgtaaat
     6601 agtaatacat ctttattgtc tatattatta attaaatttt taaaatctac aataacttca
     6661 tcatagcttt tatctaactt catatcgaaa gaaatagcac tgcactttcc aataatatca
     6721 ccaactgctt ttaccatact acttgctgta gtttctccat gcatagcaac tataatatta
     6781 atgttattca ctattttatt ttgttcatct tgtttagctt gtactatgaa taatgtaata
     6841 tatgctacag tttctataga taaaaataaa tcaaattcat tttcaattat tccaactatt
     6901 tttagtgctg ttttaaattc acttctgtta tttcttctaa tttgattcaa atctatgttg
     6961 tttgtagagc tacttttatc caatacgctt tttatataaa ttgccattga cttaaatatt
     7021 gattcactta tgtctatttc ttcatcttct ttaataattt gtcttattga ttcacacatg
     7081 cttttaaact taacattatt aaatagagaa gcatctgcta tattcatttc tacagattta
     7141 ttaagattag tatttactgc attaataata tttatatcat tgtagttaat aacttcttct
     7201 tctcctaatg acgtaaatac aaaatattct attccatgtg ttataacttc atctgcttta
     7261 atagacttct ctctataatc atcactcact tcatttatag atttatcaaa atattttttc
     7321 tctatataaa ctggttcttt attatctctt ttatatctta ggtatccatt tgcaacagct
     7381 aattttatat cattttgaag ttgacctaca ttatttttac aatcataaag taatagtgat
     7441 ttaattattt ctttatgtac ttttattgaa atagaaatac tttttgattc ttccttgaaa
     7501 aaatttttga ctaattccat tctttcaatc attgtcctat ctctcagtga tggtatttct
     7561 attttcattg gtattcttct attgaatgta tctaatagtg ttgatgtcgg attttctgta
     7621 gtagcacata ttattaatac cttagactct ctttctttcg ttgcatctcc tagtctttta
     7681 aatgttccct tatctataaa agtaaacaac atttcttgac cttctgctgg tagtctatga
     7741 atttcatcta aaaatagtat ccctccattt gcaatttcca aaagaccttt tctggaagat
     7801 tctgcacctg tataagctcc ttgttctacc ccaaaaatat gtcccataag taattgtgca
     7861 ttattagcat aatcagcaca attaaatgtt acaaaaggag ctttattttt tctaattcca
     7921 ctatctatag aataatcgta catatatcta gcaatcatac tctttcctac tcctgtttct
     7981 ccatagataa tagtatgaag tccatttgta ggatagagca ttgcgacctt gcatttttct
     8041 attataggtc ttaaactatg ttctttacct attagtgttt caaaactatc tttttttatt
     8101 ttgttttcta cttttgttcc tttttttatg ccaagcaact cactttgtgt atcaagcaat
     8161 tcattcttgt taaggttaag atttgcaggg atataataca caggtcttcc ttttatcttc
     8221 gtaaccatac cctcattgaa taattggttt aaatatgtac ttatattgtt tcttgcaatt
     8281 cctgtctttt ctgataattg gttagctgta atcccttttt cattttccct atatgatgat
     8341 tctaaaactt tcattacttt atctttttta tatatcatta caagctcccc tacattatag
     8401 tattttatta actaaaaccc ctatacacac tttatttttt aataaactct tattctttaa
     8461 ctaaggttat aatttatcaa actttcctta ctttaaatta tagaaaatta ttgtagaaaa
     8521 aataagatta ttgctcattt tttctacatt actattttta attttatagt actttcttaa
     8581 caacatactt tacttgtttt taattttatt atatttgaat ttattatatt tgaaaagtag
     8641 aaataaaaaa ccctattttg gagtagtttt aaaatttctc caaaaatagg gttttagttt
     8701 gtatttcaaa taaatacctg ttcaagtatt tttatactaa acaactttta aattttgttg
     8761 atatttattt atttctgatt tagaagaata tacaaaatgt ccatctgaaa catcaatcca
     8821 acttaatgac ttttcatttg aacaatcata tccattagaa ttatactcta ttctatttct
     8881 tgacttagca tatctagggt caggaacagg aattgcagaa agtagtgatt ttgtatatgg
     8941 atgaatagga ttattataca attcatttga atctgccaat tctactaatt ttccattata
     9001 cataactcct atacggtcac taatatgtct caccatagat aaatcatgag ctataaataa
     9061 acaagttaaa ttcttttctt gttgcaattt tttaaataaa tttactattt gtgcttgtat
     9121 agacacatcc aaagaagcta taggctcatc agctattata aattctgggt ctactgaaag
     9181 agctcgtgct atgccaatac gctgtctttg tccacctgaa aaatcagatg gatacttatt
     9241 tgcataactt ctatcaagtc ctactagctt tagcatctca tatactttat tcattctatc
     9301 tgttttattt ttacagatac cttgaatttt tagtggctct gaaattatat ctgcaattgt
     9361 catacgtgga tttaatgatg aagtagaatc ttgaaaaata atctgcatac ttttgtttac
     9421 atcttttttt ataaatttgt acgttttttt atctgatatg caatttcctt tgtaaattac
     9481 ttctccacca gtagattcat gtagtcttat tatagtttta ccagttgtag attttcctga
     9541 acccgactct cctaccaatc caaatatctc acccttatat atatcaaaac ttatatcatc
     9601 aactgctttt acagtagtac ttttgtccaa atgaaaatat tgctttaagt tttttatttc
     9661 cagtattttt tctctattca ttagaaatca cccttccaca atttactcta actggtacat
     9721 ctacttcagg tgcatctggg tgcaataacc aagttgctgc actatgtgta tcattaattt
     9781 taaacattgg tggctctttt tcataatcta tcttaagtgc atttttattc cttattgcaa
     9841 aagcatctcc ttttggtggg ttcaataaat ttggaggcat tccaggtata ttatataaat
     9901 agtcatcctg cgaatctaaa gtaggtaatg aacccaacaa cccccaagta tatggatgtc
     9961 tagggtcata aaatatatcc tctacagttc ctatttctac aatctttcct gcatacataa
    10021 ctgctattct atcagctata gttgcaacta ctcctaaatc atgagttata aatattattg
    10081 acaaacctat tttatgttgt aaatccttta ttaaatcaat tatttgagct tgtatagtta
    10141 catctaaagc agttgtaggt tcatcagcaa tcaatacatc tggattacaa gcaagtgcta
    10201 tagctattac tatacgttgt ctcattccac ctgaaaaatg atgtggaaat tgtttaaatc
    10261 ttttctcagg attatcaatt ccaaccaatt caataagctc tatggctctc ttttttgcct
    10321 cacttttact tataccttga tgaatgatta cagcttctgc aatttgcttt cctattgata
    10381 tagttggatt gagagatgtc atagggtctt gaaatatcat tgatatattc tttcctctta
    10441 tatcttccat ctctttttca gacttcttaa ccaaatcact tgatttaagt aaaacctctc
    10501 catttttaat atatccattg tatgggagta tcctcatcaa actcttacac agtacagatt
    10561 tcccacaacc agattctcca actatagcca atgtctcacc tttttttaga ttaaaagata
    10621 catttcttac agcctgaact tctccttctt ctactttaaa acttacagat agattattaa
    10681 cctccaataa atgttccaaa aaccatcaac ccctatgcta ttcaaacctt agggatatat
    10741 ttttatccct aaggtttcta attaatttca gtattattca attgtccagt cagctatgtt
    10801 ccagaatata cctacaccat gatgacctaa aactgtatca ggtgttaatc cttttatgtt
    10861 tggttttcca acatatattg catctatata agcaataaat gtataaggca tatccttagt
    10921 catttctact tggaattgtt tatataactt taatttttca tctttatctt ctgtttctct
    10981 tgctttttga agtattttat ctatagtagg attagagtaa gcactgtaat ttgcaccttt
    11041 atctgttcca aatactttat atgtatggtc atctgggtca aatggacttc cccaacctat
    11101 taaatgagca tcttgattag cccaatctgt ttcagttaca acaacagctt tagcatcaac
    11161 accaatttct tttaactgtt gtgcacatat tttagccata tcaactctaa cttggtcact
    11221 ctcacctgct gttatctcga aagctaactt agtaccttct ttttcataga ttccatcaga
    11281 acctaatttc caacctagct tttcaatttc ttgtttagct ttttctggat tatattcaaa
    11341 cttttctata tcaggattat tatatggtcc catttgtaaa ggtgaatatg ctggtacacc
    11401 atgtcctaat aatacactgt caactattgc ttttctatct attgcatagc ttaaagcatt
    11461 tggtaatcct ttagcttttt tatctttaaa gaatttagaa ttaaaattgt ataatatacc
    11521 tctatagtcg gctgttttca ttatatttac tttaaaattc ttttcgtctt tttcaaagtt
    11581 agacatatct ttaggagtta tttgagctag gtctaattct cctgatttta attgcattgc
    11641 cttagcctta tcatctggaa caatcttaaa gactacttta tctaatcctg gttcttttac
    11701 aaaataatca ctattcttaa caagtgttat actttgacct ttgtcccatt tttcaagttt
    11761 aaatggtcca gtacctattg gtttttgatt aaattcatca gtagctatat ccttaccttc
    11821 caaagcatgt tttggtaata ctcctactgt taaataatca agcattgctg tatttggagc
    11881 ttttaaagta atttttatag tattatcatt aaccacatca atttttgtta tatcttcata
    11941 atttgatgct atctcagaag cattatctgg attcattata gtttctattg taaacttaac
    12001 atcatttgct gtaaattttt ctccatcatg ccacttaaca tcatctctta aattaaaagt
    12061 atatgtattt gttgcctcat caaatttcca gtcctttgct agacaaggaa ccaccttatt
    12121 attttcatca tgagctgtta atccattaaa tattaacgaa tttatttctc catgttcata
    12181 tagtgcagga tttatacttg tatagtcatt acttccataa acaagcacct ttccatcttt
    12241 aggcgtatct gccttcttat ctttgtctcc tccactagaa caacctgcca ttagactaag
    12301 tatcattact aaacttaaaa cttttaactt ctttaatttc atgatgctcc cccttttgct
    12361 ttttataaaa tatttttcaa agctttgacc ccatttattt aaatagatat ttcaaatacc
    12421 tcagtatttg cttatttaat actcaaatgc tgatttataa attactagat tttttattat
    12481 tattctttct tatataatta cctatatttg ttatacacac caatgtggta accaaaaata
    12541 tacccggaat cagtataatc caccatctat ttgaaagtaa tgcttcctct gacaaagata
    12601 acatacttcc ccaagaaaca atttctattg gtaaaccaat tcctaaaaag ctaagtgtcg
    12661 attcagtacc aatagctgcg cctatgtttg ttacaaccat gaacattatt gatgccacaa
    12721 aatttggaag taaatgttgc tttaatacat agaagaatcc tcctcccatg ctcttagctg
    12781 ctagtatgta ttcgctattt tttatttgtc taacttctgt tctcactatc ttagatatat
    12841 tcatccaact tgttatccct attactatgg acatagatac tggatttgaa tttccaagta
    12901 tagcttgtac aaatattata attaatattg aaggtatact aagtatgatt tctgtaaacc
    12961 tcatcattgc atcatctaca agttcagatg ctgaaccact tatactacca taaacaattc
    13021 ctataacagt tgatattaca gtggaaaata gtcctataaa taaagatatt tttcctccat
    13081 accatatcat agaatatata tctctaccca ttgagtctgt tccaaataaa aaagttttat
    13141 ttggagctaa attagaactt gctaaatcca tgtatgttgg ttcatgtgtc attataaaag
    13201 atgagaatac acttccaatg actatgattg ataaaataat aattgatata tatggtaatt
    13261 ctttaatttt tttagataag gttttctttg tttctatatg ttcatcttct gctactaaaa
    13321 tataattttc tcctacaatt tcaaaatcac tatattgtgg cttcattagt atcacctcta
    13381 tcatatttca ttctagggtc tattttattg tttataattt gagctaacat attcccaaat
    13441 actactaaag cccctgttat taagcataaa actaataaca tattataatc atgatacttt
    13501 gcactttcaa aacaaagtgt tcctaaccca ggataagaaa aaactttttc aactacataa
    13561 gttcctccta gtatatgagg tatcgaaatg gccattatac taatatatga tggcattata
    13621 tttctcaggc aatgcttaaa aacaatagtt ctattattta aaccttttgc tttacataaa
    13681 agcacataat cttctcttat ttcctctagt aatttatttc ttatcatata tgtgtaatac
    13741 catagatggc ttaatataag tactgtaaga ggtaagatta gatgtgatat cctacttaat
    13801 atactggact cttctcccat tgaataagca ccactacttg gaagtatact taagtttata
    13861 ctaaatataa gtatgagtac taaggcaacc caaaatgatg gaatacagtt ggttattgtt
    13921 cctaatttgc atatgattct atcaatgagc ttatcttcat gtaatgtaca aaatattcct
    13981 aacaataagg ctagcacaaa ggtaagtata tatcctgaac cacctaatat tatagtgttt
    14041 atatagacat catttataac agaagtaaca ttttgtttat atttaaatga aattccaaat
    14101 tccccttttg atgcatttga aatccatttt atatattgac tgtatattgg ttcatccaaa
    14161 ccaagttttt tcatagcatt ttctttttct tgtgtactca ttctttctac tccatcccca
    14221 tagtaagaga ttaatgggtc tcctggagca agtcttgcca tgtaaaaaac aataaaagat
    14281 aataaaaaca ttactaaaat aaactgcaac aactttttca gtatatcctt taacaaaccc
    14341 caacccccct ttgttgcaat aaaaaaaacc acataaaagc aaacgtaaag taacgttaaa
    14401 accttcgtgg ttccattatc attaaacaaa attaaatttt ctatatttat ctatgagtta
    14461 attttagcat catttattaa ctgtgtaaac aaacttttta aaaattgtta aaatttcatc
    14521 aaattatttt atttatacct aaaataaaat ttttatattc tattcttcat tattttctat
    14581 aaaattataa aattttaggt tgactatata agaaaagtat attatattag tttgtatgca
    14641 aaccatttaa gaggtgattt tattgaaaag taattatgac tgttttagaa tagccatgct
    14701 cttaaaagaa ctttattcta aaactatgta cacagtagaa gaaaatttta aagaaaatgg
    14761 attaacacac caacagatta tagttataaa actgatagct cataatcaag aacttacaat
    14821 atcacaacta tgtgatgaaa tgtctttagc taaaggtacg gtttcaggca taatcagcag
    14881 attagaacag attggatata tagaaaaatt caagaaatct aatgataaaa gaaatacata
    14941 cgttaagttt acaacaacag gatttgaatt tgccacaaat tttaaaatta aaatgcaaga
    15001 aagttttgat gatattttta aaaattgtga tgaaaacgaa ttgagtgact tagtaaaaaa
    15061 tcttagaaat atattggcaa aagtaaagga gagataatta tgaattataa attaatactt
    15121 gcaatagtat ttataacatc agctttaatt ttctacacta taggtgtatt tggagaaaga
    15181 aaggcaaaaa ttttaaaaaa gaaacatgta attatatttt ggcttggttt tatatttgat
    15241 actttaggaa catttacaat gagtaacata gccaacagcc atacttttga agtaaaatct
    15301 gctttatcac aaaaccttca tagtattaca ggtcttcttg caattgtgct tatgcttttt
    15361 catgcaagtt gggctacttt tgtcctatac aaagatgatg aggaaaagaa aaaattcttt
    15421 cataagttta gtatagttgt atggactata tggttagttc catattttat aggtatgttt
    15481 attggtatgg ctggatagta aaataaaaag tgtgtaagaa atactgtagg taaatttgta
    15541 taagacaaat ttatctacat ccttctttca cacttttaca tatttattta taatactcca
    15601 ttatttcttt catgtttctt aatacctata tagaatattt aattgtttct atagtatttt
    15661 attattttct cacacttttt catatatata ttgttttact atttcttcat atgtatatat
    15721 gaaatattcc attatttcta taaatgtttt attatctttc tcatactttt tcatatatat
    15781 attgcttcac tattttttct tatttcttca tatacatatg aaatatttca ttatttctat
    15841 aatgttttat tatctttctc atactttttc atatatattg cttcactatt ttttttactt
    15901 ctttattaaa tacttatgta taacatacat ttaataaacg ctaactatta aacacctaat
    15961 acttgagtta ctcctggaac ttcactcacc aaaacatttt caataatagc tttgattgtc
    16021 atagtcactc ctggacaccc actacatgcc ccttgtagtc taactaaaac cacaccattt
    16081 tcatttacat ctataagctc tacatctcca ccatctcttt gtaatactgg ttttatcttt
    16141 tcctcaagca ctttttcaac tttttctctc atagctattc cccccagaaa tattttatta
    16201 gcaaaattat accaatggtt taaatttttg tccatctaat gatttatcaa attcaatttt
    16261 tgcttcatca ataattttag tatccataaa tacatcataa gcactacaag ccagtacttt
    16321 agctgaattt atcatagcag aaaatcctat atttgaacct gctgatgaac atgactgcca
    16381 tgtatgactt gcaattccaa caggccaagc tgccattgca aattgagcca ctggtattac
    16441 ataacttaca tctccaacat cagtagaacc agctagtcct ttatctttgt caccgtcaac
    16501 tatttccttg tgtatatagc tttctacaat agatttatca ttttgtaatg atgttgctac
    16561 acctaatctt tcttctctag taagagtatc acacaacttt tttgcaaatt cttcttcttc
    16621 aggagtattt ttagggcaac caacaaaaac catattgtca taaactaaat cagtcaaaca
    16681 ttggtttggt atatagtcat atattccatc tgtaacctta tactccattt cagtttctgt
    16741 catcattgct gctccttgag ctacttttat taatctatca agaacgtgtt ctgcatcctt
    16801 tgctttttta cctctaataa agtaccaact ttcagcaaat ccaggaacta cattaggtct
    16861 tccaccacca ttagttataa catagtgcat tcttatagag tcaataacat gttctctcaa
    16921 ataattagct ccaacattca taatttcaac agcatcaagt gcgcttctac cattatgagg
    16981 agcttgagct gcatgggctg ttatcccttt aaatttaaat tttacagata agtttgccag
    17041 acttccacct ctccaaggtg catttatatc aaaaggatgc catgtaaatg cacaatctac
    17101 atcatcaaaa cagccattta tacacataac tgtctttcct ccaccttctt cttcagcagg
    17161 acaaccaaaa tattttattg ttccattaaa tttattttct tctattaatt tttttataga
    17221 aacaactgct cctacacctg ctacacccaa caggttgtgc ccacatccat gtccagcttt
    17281 tccttctacc aatggctttc tttctgcact tacacattgt gatagctctg gtaaagcatc
    17341 atattctcca agtatgccaa tcacaggttt accctctcca aaacttgcta taaatccagt
    17401 aggtaaattt tctacttctt caattttaaa tccttttgac tttaggtatt ctttttgaat
    17461 gctacttgca tacttttctt caaaggctaa ttctggattt tcccatattt tcttagataa
    17521 atcaattaat tcttctctta tttcttcaac attatttatg atatactctt tcataatttt
    17581 cctccaaata tatgtatttt tgatatgctt tacagattat attatatact aacattctat
    17641 ttattcacaa ttttttgtta attaatttta ttcatatgct cttctatgga gtatatctta
    17701 tcttcacttt gcctcttcat aaatttaatg tactctatct tccataaagt agtttcagtt
    17761 ttctcattta acataaattc taatatatta atttctctca ttctaatatc ttcataaaat
    17821 actacactac ccaatattgc caattctaat tcacttaatg atgcaaacaa cattaaatac
    17881 tcttctccaa ataaacattc agattcaaga atatcgataa agtctttttt agcattgata
    17941 ttttccttta ttattcttat tttttcaatt atagatgtac tatctaatat ctcattgaat
    18001 atctctctaa acgattcatc atccaattta ctttcatcat caacaatata atcttctaag
    18061 tgttcctttt tatttttact cacagttacc atacttttaa aagtgttact ttttattgag
    18121 cttagcacag attcaataaa tatcttttca tattttttaa tatatcttat tacatcttca
    18181 tcttctatgc ttaatctttt tattaatact tcaattgctt ccataagtag taaagttatt
    18241 tttttattta ttctaatttc tctgttaatt tcttcaaaat cattataatc caaattacta
    18301 tctacttcag tgttaaaact atcatctact tcaatcaatt caattatatt actttcttca
    18361 gagtctataa ctttatcttc agtaatatta ttgtacatat ttttaaattg tttttctaaa
    18421 aattcatact catattttga aatttctaaa ttagtcactt catttcctaa aatagtagaa
    18481 aaaatacaat tattcataat aagttcaaat aaatttacaa gtataacatc acaatctatt
    18541 ttataagtca tcccatagtc ataaaataat ttttctctat cttttttctt aaataggtta
    18601 caaaatctat tttctatatc tatactctca atataattct tcacataata aataccctta
    18661 acatccatat catctagtgc caatggataa tctatactta ctggaacgtc ctgagcttca
    18721 aattttacaa tataactttt aaagaaactt gataactcca tcaaggtatc attatatgct
    18781 attgaattag tatccaattt tctactcatc attagtttat aaaaatcttt tagcctaact
    18841 aactcttcct ctaatatttc aatgcctcca aggtacactt ttttaacatt cttagcagtc
    18901 aactccacaa ctcttttttc tatatctcca agcttattta tatatgcatc aatagcatac
    18961 catattgaaa tcaacagttt ttcggcaact tctgttttta ctgatgtgct ttgaccattt
    19021 gtatattttt taatcaagtc cataagtatt agagatactt cttgttgtat attatatact
    19081 tctctttcat ctatcacttt acaatttaga cattccttaa gtagagatat ggaatattgg
    19141 ttctttatta aattattttc gtttatatta atattactta aattatttaa catacaaaat
    19201 ctctcctatc atatattaaa cttctccaaa tttaaatttc tttgcaaatt cgtctgcttc
    19261 tcctcttaca ttgtccatat tgcctgaaaa tttttcataa aattcaccca gtatttctat
    19321 aatctcatca tcacatatct tgtcatcagt ttcatttttt aaataataaa aaatttcttg
    19381 catatcatta atagcctcta aataattgtc cacatttgta tattgtgatg tatatatatt
    19441 ttctattaac tgttttgtaa cttttatatc taattctatt cttccatacc cctttaaagt
    19501 attatctctc gaatttatta tatcttttac atcagattca gaaagagcta gaccataatc
    19561 tccagattct atattagttt caagcagttc atttctttct atttccttta aattctgatt
    19621 tatagaactc aaaataatag gtgatatcat ttttaaattc tcctttttta ttttttccat
    19681 tgactttttt tgttttttat gctatactta aattaatgta tattatttta ccaaccactt
    19741 ataatagtag tgttaaaagt ggtttttgtc aactcaaaat tactatctca attaaacttt
    19801 tcaattttct caacaaaaaa ataccatcat ttagatatat aattttctat aacaactttt
    19861 aattcagtag ttatttacta aaatctaaac aatgatatat ttattaattt tatttatctt
    19921 ttttggctct acttctactt ttctttacat ttgagcaatt tctacaagaa ggtgaacagt
    19981 attgagattt agaatttgta ggaatataaa ttttaccaca gtacttgcat ttatttaaag
    20041 aagaaggttc acttgtaatt gcaaaggcat agatagtctc aatagctgtt ttcaaagaat
    20101 caaactccca tgcaataatt ggtttatcta attggcttat agaaaatcct atcttttgag
    20161 gattaaaccc tccagacata atagtaacac tttcagttaa tagattagat gtatttttat
    20221 atacacaaat ttgattaaaa tgcttacaca tatctttagc aaaacttaca atccaatcta
    20281 gttgttcagt gtagaacttt gaaaatacta aatctaaaat caatggtctc tttccaaaaa
    20341 actttggaga ttcttctctc tttgatacat ctatacccct tctatacttt ttaaaataca
    20401 catcttcatc acttgcaaat ggtataaaca tgtttacata ttcatctgta ctcaatatat
    20461 tctcttttgt aatataattc ttttcaatca tcagtactct ttcttctcca actatatctc
    20521 tattatatac acttgaatat attaaaccca ataatccata cttttttaca aattcaagta
    20581 cttctttctt tagtttcatt tcttttgtct cttttgatat atcttgtaat gcctcttgtc
    20641 ctatgcgaat tatatctact attatatcat ctgctacatc aaatgggtta tacatggtaa
    20701 acacagcatc ttctgttgga actatatata attcatcttc ttcagtaatt ttatattcat
    20761 aatcactata cctaatccaa tcacttgcaa aattccctat taattctatt tttttattca
    20821 taataatcat tcacaccttt caatgtaata tataacttca tattactatt acacttttag
    20881 tttattccat attttctaca ctgtcaacta ttctttttat atttttagca tattaaaatt
    20941 tataatagcc tttataatgg atttttacag ctaaatttct tgtttattga tgactaattc
    21001 ataattaaat ttataaaaac tatttacata taaaattaat ctctttataa ttgatatgta
    21061 atatattttt acacttacat attacatatc aattataaag agaggtatct aaaatcatgg
    21121 atagaaaacg tgaattaaaa caattataca aagaaatgaa atttgacaca ggtgtattca
    21181 taataaaaaa tgatattact aaaaagattt accttggaaa aagtaatgac ataaaaagca
    21241 aatttaactc tctcaaattt cagcttggtg ctggctcatg tatgataaaa gaattaaata
    21301 atgaatggaa aaaatatggt gaaaaagcat ttacatttga agttttagaa cttataaaac
    21361 atgatgataa caaaacagag aaagactatt tagaagaact agatatttta gagatggttt
    21421 ggttagaaaa attaaaggaa gataatactt atgaaattta ttccatataa aaaataatat
    21481 aatataagaa aagtagatag tgtctcaaaa tagacattta gttcataaga tactttttta
    21541 tataaaataa agtatctttt cataatttta acaatgaaaa agaggcttat ctccatttga
    21601 tagcctcttc ccatatattt tataccatta taaaatgaac aatctactaa ttttcaatgt
    21661 atatattcat tttactaata acactccatt acttctttac agcatccaaa cattgctcta
    21721 taatactctt atcacttata cctattgctg ttactttact agctccactt aatatactct
    21781 tatcactttt atttgaaact aaaacaacag gagcattttt tgcaagtggt gatgcaacta
    21841 aagcataaac taaatcatct ccacttgtta tatagaactc ttttgctcca ttatagaact
    21901 tttcaataac atttttatta gtttgatatc tatcaacacc accaattcta gttgctctat
    21961 atctgctcac tatgtaatca cttaatgtag actctcctcc aatagcatat actggaacag
    22021 gagaaactcc aaaccatggg tctaagtctg tgtcttcttc tgctgaaggt atatcttttg
    22081 tcaatattat aggagctcca tctctataag caacagatgc aacacttact gcatcagctt
    22141 catccttaaa tccatttact aaaaacatct tatcactgtt tttaattttt tctatttcac
    22201 ctgcaatttt ataacttgta tctactctat ctacaccatc aattcttata acttgcattc
    22261 ttctcataag taacattttt tctacatctt tacttataga attttctcca cctattacat
    22321 atatcttagt aactctattc agtctttgaa gtgttgcttc tggtatatca tccttattag
    22381 ttaataaaat agctgcatta tttactcctg caagaccact tgcagcaatc ccatctacca
    22441 ttgtagagtc tgcattgata agaactgctg ttgtaaaatc ttgcttatct gaaataagcc
    22501 cagctgtttc atatttatct actccttgaa tattttcaat cttatctagt gcgtttattg
    22561 atgttggaaa actaacactt aacagtaatg ctgaaatacc taacgccaat acctttttac
    22621 taatcctcat aaattacctc cctaaaacaa atatatttag tattagtata atataaaatc
    22681 atttaaaaat agtgtaaaaa tagttacact tttttataat ttaaataatt ttagactaaa
    22741 cacaattcat ccattaaaat aacttcattt attctatatg ctaagccatc ccacgttttt
    22801 tttgacatat ctacagatgt tttcttattt gagttattca ataaagattg tatcttagaa
    22861 tctattgcat tatacaaatt attttcaaaa tcatataact cttcatcttt tggaatacct
    22921 tctttctcca taaaaggtag agaaacatat tctatctttc cagaattatt aatctcacta
    22981 ccaatccact ctttaacacc aagaatatca gtagtgatta catctgtgcc acaagataaa
    23041 gcttctaaaa ctacaactgg taatccctca taaaatgatg gtaacacaaa tatttgtgac
    23101 ctgttgaata gctcagctaa atctctctgt tctaactttc ctaggaaatt cattttaaat
    23161 ggagacttac ttgccagttt aactatctca ttatatgatt cgatatcgct tccagtacct
    23221 gcaaaattga cttctattaa atctttacta tattttaatt tagatatagc ttttataaat
    23281 ggaatcagtc cctttgattt acatatctta cctgcaaata ctatttttat tttatcatcc
    23341 tttattttat aatttttatt ataaaatata tcatcattat aaccactacc aattacaact
    23401 actttacttt cacttactcc aaaagtcttt ataatatcat atttctgatt ttcatgaaga
    23461 gcaaatatta aatctaattt ccttatattt gctattatgt attctttctc taaatcaatg
    23521 gtatttaact gtcttaaaca agttccatga caaatagaca taacctttat atcttttacc
    23581 atttccctaa caaatgcagt taagaggtac aaatgatgac atattataag ttctggttta
    23641 aaatcattca tagccttatc tatatttact ttaaattgat atttcaatct atttatcatg
    23701 tctatattta aatctttata tcttgtactt tcatatggca tagaatcact catacctaca
    23761 acaggaaaat ttaactcccc acaattatac tttactggat aaaaacttac ttcacttgga
    23821 aaacaattta catcatcatt tacatctata cctgcaatta cagcttgctt atgaccgatt
    23881 ttttcgaacc cttttatcat accagacaaa tatataccac tacctgtact atttggtttt
    23941 tgtgttatta tatgaagaat tttcactttt cacttgcctc ctttctaaaa ttaaatagtt
    24001 ttatacagtc taatcattat atatatctaa atataaacaa gatgaatcat cttttacttt
    24061 aaatcttggc actttaaccg ctttatagtc attatcttca aaatccctta ctttattatg
    24121 tatatatcct ataccaaatt tttctgctat acgtatcaat tcttcttctt ttattagatt
    24181 atatctatca catgcacttg taaaaccatc actcgttagc atcaattgaa aatctttttt
    24241 tatttcaatg tatccatata tagaattttc cacagcttcc tcatcaaaat ccaatatcca
    24301 atagccctct tttgtatttt tcttcaatct attgcttact attgtatcca taacactagt
    24361 tctaatctca tcaaaagcca tctcttccaa gttagaaagt ttactcattt catcaaatac
    24421 agtattatca aatttacaaa tactcctatc ttttataact ttggaatctc catttttaat
    24481 aaacaaagca caatctccta ataaaaaata ctctacttta ttttcatgaa attttacaac
    24541 aactatagaa ctagatggta aatctaattt ttcaacctta aacccattta atcttagttc
    24601 atactctttt tttacttcta ttattccagt attaataata ctctttaatg attcttcttt
    24661 atttatattt ttatataaat acctattcca ccactgaaca taccaccttg cgtcactttc
    24721 agttgacact aaattttttc cattaagtcc tgtagcacca tctaaaaccc atgccccaaa
    24781 tggatttata ccgactacat cttcattaaa tttagctgtt ccttgattac ataataggct
    24841 atagtttctt atatttaaag ccatatcaac ctccaaattg aatatctaaa aataaagttc
    24901 tatttaaaat tatacagcaa ataaaaaaat ctacaacaaa acttgttgta gatttttact
    24961 taatctatat tatctttata ctttcagtat tttaatgatg gaataatcta tttttagtaa
    25021 atgccatgac tatgttatac ttattacaag ttaatataac attatcatca cgaatagaac
    25081 cacctggttg tgctataaaa cttaccccac ttctttttgc acgttctata ttatctccaa
    25141 atgggaaaaa tgcatctgaa ccaagtgcta ctcctgttaa agttttaagc catgctcttt
    25201 ttccttctcc agttagaggc tctggttttt cttcaaaaaa gttttgccat atgccatcag
    25261 caagcacatc catataatca tcagaaagat atacatcaat agtgttatct ctatctggac
    25321 gacctatatc ctttttaaat tttaagttta atactttagg atgttgacga agataccaag
    25381 tatcagcttt gttacctgca agtctagtac aatgaatacg tgattgttga cctgcaccta
    25441 cccctattac ttgtccatct ttagcataac aaacagaatt tgattgtgta tattttaatg
    25501 taataagagc tataattaaa tcacgttttg cactatctgt aaaaatttta ttatcagttg
    25561 gaatatcttt taataggtct tcatttatta atatttcatt tctaccttgt tcaaaagtaa
    25621 ttccataaac atcctttgtt tcaataggtt caggagtata attttcatct atttttacaa
    25681 tattatagtt acctttacgc tttcctttta atatttcaag ggcttcatct gtatatcctg
    25741 gagctattat accatcagaa acctctcttg ctataatagt tgcagtctcc ttatcacata
    25801 catctgaaag tgctataaaa tcgccataag aagacattct atctgctcct cttgccattg
    25861 cataagcaca agctaatgga gaaagctcta aatcatcaac aaaatatatt tgctttaaag
    25921 tatctgataa aggtacccct actgctgctc ctgctggact tacatgttta aatgaagttg
    25981 ctgctggaag acctgtagct tcttttaatt ccttcacaag ttgccaacta ttaaatgcat
    26041 ctaaaaaatt tatgtatcca ggttttccat ttaatacctc tataggaagt tctccattct
    26101 tcatataaat ctttgatggt ttttgatttg gattacagcc atactttaat tctagttctc
    26161 ttgccatata ctcatccctc tctataataa attaatcata agtttgatta atcctatatt
    26221 aattaaatta ttcgtactaa taaaattata tggcatattt atccattaat caactatatt
    26281 ttttaaaact cctattcctt cgactttaca ttcaactata tcgccatgct ttaaatatac
    26341 ttgaggattc atccccatag caacccctgc tggagttcca gttgaaatta tatctcctgg
    26401 ctttagagtc attcctcttg ataaatcatt tattatagta ggtatatcaa atataaagta
    26461 ctctgtatta gaatcttgtc ttacttctcc atttactaca ctacttatat ctaattttaa
    26521 aggatgttca aattcttctt tatgtactat acaaggtccc atagatgtat gtgtgtctaa
    26581 gctttttcct ctaaaccatt gaacatgttt atcttgaagt gctctttcac ttatatcatt
    26641 tactattgta tatccaaata tatattcata agcatcttct tttgatatat ttataccttc
    26701 tttacctatg attactgcaa gttctgattc atagtccaac ttctctacca aatctccatg
    26761 taagcttatc ttatcatcca ctccaattat tttgtctgct ctcttagaaa aataaattgg
    26821 ataatctggc ataactacgt tttttataac tccatctggg atttcattga catggtcttt
    26881 atagtttaga cctaaacata taatatcttt tttaggtctt acaattgggg aacataattt
    26941 aacttcttct aacttataac actttgcgtt ttcaaatgaa ttgtttttta ttttttctaa
    27001 atctcctgta gatacgtttt caataaaatc attcatatca gtatataatt tacttaatcc
    27061 taaactatta atttcatata tactatttgt ttctgaatta aatacaccta ttttttcttc
    27121 attgctacta caaaaagtca caaatttcat ttttattcct ccattcaata agttgattaa
    27181 tattatttaa tttttattag tttatataaa ttgtaattc
//
LOCUS       DAYASV010000043        27017 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYASV010000043 DAYASV010000000
VERSION     DAYASV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27017
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.043"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            35..373
                     /locus_tag="ACGI68_001780"
     CDS             35..373
                     /locus_tag="ACGI68_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HFH0138222.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGKP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(500..1789)
                     /locus_tag="ACGI68_001781"
     CDS             complement(500..1789)
                     /locus_tag="ACGI68_001781"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0138223.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFLTENNVLMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1869..2573
                     /locus_tag="ACGI68_001782"
     CDS             1869..2573
                     /locus_tag="ACGI68_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFH0138224.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHSKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKLNQKNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2665..3711
                     /gene="selD"
                     /locus_tag="ACGI68_001783"
     CDS             2665..3711
                     /gene="selD"
                     /locus_tag="ACGI68_001783"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2713..2715,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFH0138225.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3762..5141
                     /gene="selA"
                     /locus_tag="ACGI68_001784"
     CDS             3762..5141
                     /gene="selA"
                     /locus_tag="ACGI68_001784"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFH0138226.1"
                     /translation="MLPSVDEVLSDNRIVEIINEYPRSLVLESVREVIDLKRQFILRL
                     KEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLGRSLLSEDIKDE
                     LWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNAAAVLLVLSTMA
                     KGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYKEAITEDTNVLM
                     KVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKYGLSYEPTVLDS
                     IRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDKLTICALEATLR
                     MYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINIEDGFSQVGGGS
                     MPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDVRTIFDDEFDVI
                     VEELRKAFN"
     gene            5255..7138
                     /gene="selB"
                     /locus_tag="ACGI68_001785"
     CDS             5255..7138
                     /gene="selB"
                     /locus_tag="ACGI68_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFH0138227.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7243..9981
                     /locus_tag="ACGI68_001786"
     CDS             7243..9981
                     /locus_tag="ACGI68_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HFH0138228.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEHELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10100..11044)
                     /gene="manA"
                     /locus_tag="ACGI68_001787"
     CDS             complement(10100..11044)
                     /gene="manA"
                     /locus_tag="ACGI68_001787"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFH0138229.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11217..12578
                     /locus_tag="ACGI68_001788"
     CDS             11217..12578
                     /locus_tag="ACGI68_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138230.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12666..14555
                     /locus_tag="ACGI68_001789"
     CDS             12666..14555
                     /locus_tag="ACGI68_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138231.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISSVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKANTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14570..15025
                     /locus_tag="ACGI68_001790"
     CDS             14570..15025
                     /locus_tag="ACGI68_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138232.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15084..15401
                     /locus_tag="ACGI68_001791"
     CDS             15084..15401
                     /locus_tag="ACGI68_001791"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0138233.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15426..16688
                     /locus_tag="ACGI68_001792"
     CDS             15426..16688
                     /locus_tag="ACGI68_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0138234.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16793..17857
                     /locus_tag="ACGI68_001793"
     CDS             16793..17857
                     /locus_tag="ACGI68_001793"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0138235.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YAGNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            17911..19089
                     /locus_tag="ACGI68_001794"
     CDS             17911..19089
                     /locus_tag="ACGI68_001794"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0138236.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
     gene            complement(19280..20338)
                     /locus_tag="ACGI68_001795"
     CDS             complement(19280..20338)
                     /locus_tag="ACGI68_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFH0138237.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKNGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            20405..20776
                     /locus_tag="ACGI68_001796"
     CDS             20405..20776
                     /locus_tag="ACGI68_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFH0138238.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            20813..21487
                     /locus_tag="ACGI68_001797"
     CDS             20813..21487
                     /locus_tag="ACGI68_001797"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFH0138239.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            21736..22575
                     /locus_tag="ACGI68_001798"
     CDS             21736..22575
                     /locus_tag="ACGI68_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HFH0138240.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            22819..22915
                     /locus_tag="ACGI68_001799"
     tRNA            22819..22915
                     /locus_tag="ACGI68_001799"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22853..22855,aa:Sec,seq:tca)
     gene            23065..23772
                     /locus_tag="ACGI68_001800"
     CDS             23065..23772
                     /locus_tag="ACGI68_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFH0138241.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(23914..24750)
                     /locus_tag="ACGI68_001801"
     CDS             complement(23914..24750)
                     /locus_tag="ACGI68_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0138242.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSEAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            24938..25843
                     /locus_tag="ACGI68_001802"
     CDS             24938..25843
                     /locus_tag="ACGI68_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HFH0138243.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            25852..>27017
                     /locus_tag="ACGI68_001803"
     CDS             25852..>27017
                     /locus_tag="ACGI68_001803"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0138244.1"
                     /translation="MEIRYAKEEEIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIENDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNN"
BASE COUNT        10765 a         2828 c         4602 g         8822 t
ORIGIN      
        1 gaaaatcaat atcaaataaa tgggaggaaa tctaatgaac ttattagtga ttggtggtca
       61 tgaaagaatg gaaaaagatt atatggttat ggccaagaaa aaaggttata agacaaaggt
      121 atatactacc atgtcatcta aattaaataa ttctatagga aaaccagatg cagttgtcat
      181 acttacatca actgtttcac ataagatgtc tagaacggtt gagtctcaag ctaaaaaaca
      241 agatatatta atcgtaaggc ataaaaatag tagtaaggta gcatttaatg aatgtctaga
      301 tatggttgat gaatgtctgg gaaattgttc aaaatgtaag cttaaagaca aacaaaaaca
      361 aaaaaatata taggtataaa aaatacataa acataaaatt aaaagtactg agataatagt
      421 tataattatt atctcagtac ttttagattt ataaggataa gtatagtata aattttttat
      481 tataatttcc aactacttgt tactttttca tatttttttt acttctagct ttttttcttt
      541 ttttcttctt tatatttgaa gctattataa ataaaactaa aagcactaga atacctgcaa
      601 taatcttaac aaaagtcatt aaaacattat tttcagtcaa aaatggaaga ctgctatcaa
      661 gattattttt tgcaattaag tctatactgt tttctagttt accattatta tatacattta
      721 aagtaccaac cttagctcct ttttttatag gcaaatcaat tttatttaac tctgttttgg
      781 tatcataatt gccttgagat tcatcttttt ctaagaatat cctataacta ttttttggtt
      841 cataaatcaa ttcatgttgt tttgtaaaca atactttttt agtatctgtg tatttttctt
      901 tatcaacaat agtagcacac ttaaagttat caaatccata atcaatcaga atacgtgagt
      961 ctaaatataa atcagcatta gttgaattaa atacagcaac gataactctt ctgccatctt
     1021 ttacagcact cgataaaaga catttaccag cagcatcagt atatccagtt tttataccat
     1081 caactatatc atattttata tttataggtt gacctttgta tgtaatctta tcatgtgatg
     1141 tcaaaaactt attagtgttt acaaagtatc tctcataagg atatgctttt gtagcttcaa
     1201 atttaagtga cttagttttt acaatttgtc taaaagtatc attattcata gcttctctag
     1261 ctatgagagc catatcatga gctgttgtgt agtgatttgg gtctggtaat ccatgaggat
     1321 tattaaaatg agtattagta gctccaattt tttttgcctc agagttcatg aggtcaataa
     1381 atttttgtac attgccacca ccaacatatc tagctaatac aaaagcagca tcattagcag
     1441 aatgaaccaa aagagcatct agtaactgct ttacagtaaa agattcccca ttttttagat
     1501 acatactaga gccatctatt tgaggtaaat ctttaatttc tattacttga tttaaatctt
     1561 taacctcctt tagaactaaa caagctgtcc atactttagt tgttgaagct ggataaagct
     1621 tagaatttcc atccttatta tatagtattt tgccagactc atagtccatt aaaactgcat
     1681 gtttagcaac tatagctggt tcattatctg caaaagagat attagagctt cctaaaaatg
     1741 aagtaaatat caataaacag attaataaaa gtgataaatt tcttttcatt gtgagcctcc
     1801 aaattttaaa ttcatctttt agtatagcat atatttgtca ataattaaaa gaaaggagtg
     1861 attaatatat gaaaaaaaag gtaaaattta tttcgttttt tgttttattt attttaataa
     1921 tatcatcaat aatcgtttat aaatcgctta ttttcaaaga tgatatttat gttgtaagta
     1981 aaaaaaattt acctaatgaa aaaacacatg ttgtagagaa tgaaattaaa ggtgaagata
     2041 ataatcacag taaagtaact aaagacatag ataaaaattt tcaaaataat aaaataacta
     2101 tatacataag tggagcagta aatagacctg gaattgtaac tatagagtca gacaagagac
     2161 tttatgatgc tgtagaatta ctaggaggaa cgacaaaaga agcagattta aatggagtca
     2221 atctatcagt aagattagaa gatgaacaac attacataat acctaaaata ggtgaagcaa
     2281 catctgttac aagtaatgat gattccaata aactaaatca gaagaatgag tcaagaggtg
     2341 agcctaaaaa taaatccaat aaaaatatat ctaacgaatc aaaagtaaat ataaatgtag
     2401 cgacgataga agagttggat tctctacctg gggttggaga agctacagcc aataaaattt
     2461 tacaacacag agaagaaaat ggacagttta gttcaattga ggaaataaaa aatgtaaatg
     2521 gtattggaga taaaaagtat gaaaatataa aagatttaat ttgtgtagac taaaatataa
     2581 tacaaaactt acatatagat atgttattat aaatatgtat gtatatgtgt gtacatgtgg
     2641 aaatttagta aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca
     2701 acagcaggtg gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa
     2761 ttgcctaaaa atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct
     2821 gcagtttata aattaaatga tgatatggca cttatccaaa ctttagactt ctttacacca
     2881 atggtagatg acccatacgt atttggtcaa attgctgcat caaattcact atcagatgta
     2941 tatgctatgg gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat
     3001 gatatggatg tacttgcaga aatattaaaa ggtgggtttg ataaagtgaa agaaagtgga
     3061 gcacttttgg ttggagggca tactgtagat gacaaagagc ctaaatatgg tctttcagtt
     3121 tctgggattg taagtccaaa taaagtttta tcaaatgcta ctgcaaaacc tggtgataag
     3181 ttaattataa caaaaccaat tggagtgggt gtattaaata cagccatgaa agaaggtatg
     3241 gtagaacaac atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca
     3301 gcaatgagtt ttgagaaatt tgatgtaaat tcggttacag atataactgg ttttggtcta
     3361 cttggtcata ctttagaaat ggccaaagct tctgaagtaa gtattgaaat tgaatcaaaa
     3421 catgtaccaa ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt
     3481 atgtataaaa atatgcatta tgtatctaaa gatgtagaag ttgtaggaaa tatagaagtt
     3541 gcagttcaag atattttata cgacccacaa acatcaggag ggttgttaat ttcagtaaaa
     3601 gaagaacttg cagaggagct tgtaaaagat atgaaattaa atggagcaat agaagctaaa
     3661 attataggtt ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata
     3721 gagaaagagt gattgttttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg
     3781 aagtattaag tgataataga attgtagaaa taataaatga atatcctaga agtttagttt
     3841 tagaatctgt gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag
     3901 atgcatcaaa ctctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag
     3961 ttaagttaaa ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc
     4021 atacaaattt aggaaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg
     4081 cttcaaggta ttcaaactta gaatacgatt tggataatgg agaaagaggt tcaagatact
     4141 ctcatttaac tagtacaata aaaagactta ctggagcaga agatgtacta gttgtaaata
     4201 ataatgcagc ggcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg
     4261 tatctcgtgg agaattagtt gaagtaggtg gttcatttag gattccaagt ataatggctc
     4321 ttagtggtgc agaattagtt gaagttggtt caactaataa aactcatttg aaagattaca
     4381 aggaggccat tacagaagat acaaatgtat tgatgaaggt tcatacaagt aattatagaa
     4441 taatgggatt cacagagagt gtttctatag aagaattggt taatttaggt aaaaaatata
     4501 aactacctgt aatagaagac ttaggaagtg gagtttttat agatttatca aaatatggtt
     4561 tatcttatga accaactgta ttggattcta ttcgacaagg tgcagatgta gtaactttta
     4621 gtggagataa gatgcttgga ggtccgcaag caggtattat agttgggaaa aaagagtata
     4681 tagaaaaaat gaagaaaaat caacttacta gagcacttag agttgataag cttacaatat
     4741 gtgctcttga agcaacattg agaatgtatt tagatgagac aaaagctata gaaaatatac
     4801 ctactttaaa aatgttgaca tataaaattg aagaattaga agtaaaagca aataaactat
     4861 ttgaaaaaat aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac
     4921 aggtgggagg agggtctatg cctcttgaaa ctataagtac aaaggtaata tcaataactc
     4981 ctgaacatat gaatgtttca tctttagaaa aaaaactaag acttagtgaa gcacatataa
     5041 tagccagagt gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat
     5101 ttgatgtaat agttgaggaa ttgagaaaag catttaatta aataaatttg agttaataaa
     5161 tattaaattt agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg
     5221 actttagtca tagcaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc
     5281 aggtcatatt gaccacggaa aaactactct tataaaagca ttgactggaa gagagactga
     5341 tacgttagat gaagaaaaaa agagaggtat ttcaataaac ttaggattta cttattttga
     5401 tttaccaaac aatagaagag ctggaattgt tgatgtccca ggacacgaaa aatttataaa
     5461 aaacatgtta gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga
     5521 aggggttatg ccacagacag ttgagcattt agatattcta agctttttaa atatcaaaaa
     5581 tggaatagtg gttctaacta agtctgatac tgtagatgat gaatttagag agctagtaaa
     5641 agaagatatt tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt
     5701 tgattctata tctaaaagag gtatagacac tcttattgaa aaaatagata aaatgagtga
     5761 tgatatagaa gataaaaatg aaaactctcc agcaagactt aatatagaca gagtattttc
     5821 aataaaaggg tttggtactg taataacagg cactttaata gaaggaaaga taagtataga
     5881 agatgatttg gttatatatc cacaggtatt aaaaacaaaa ataagaagta tacaagttca
     5941 tggagaaaat aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt
     6001 aaaagtggat gatataaaaa gaggagatgt tttagctgca ccaaactcat tggaggaatc
     6061 aatgatgtta gatgtaaaat tatcattggt aaatcattta gataaaggat taaagcactg
     6121 ggatagatta agattgtatc atggaactag agagatactt tgtagagcag ttccactaga
     6181 taaagaatta attgaaagtg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat
     6241 agtatctaag aaaggtgata cgtttgtagt gagacgttat tctccaatgg aaactatagg
     6301 tggaggtgta ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat
     6361 agaagcatta aaaataaaag aaaaaggcga actaaaagat ataatagaag agtacttaaa
     6421 aaggaattta aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga
     6481 agaagatgta aaaagagcgc ttgaaacact tatatcagaa gataaagtat ttattatagg
     6541 aaatatgtat atgcatataa atcaatacaa taaattaaaa gaaaatacaa taaagctttt
     6601 aagtgagtac cataagaagt ataggcttag aaaagggata ctaaaagaag aagtaaggtc
     6661 taagatagag agtaatttta agactaggga gatggatatt ttattagaaa aattgtctac
     6721 agaaaatgct ataaaaatag aaaataatat agtatctcta ttagattttg aggttatttt
     6781 aaatgataag caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt
     6841 ttcttctata ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt
     6901 ggagtcaatg ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga
     6961 tatttatgaa aatgcaaaga acattttgat aaattatata aaagaaaata aagaaataac
     7021 attaggagaa tacagagatt tgattgattc tagcagaaag aattgcatga ttatattgga
     7081 aaacttcgat agaaacaaaa taactaaaag agtagaaaac aaaagaattt tgttttagca
     7141 gaaaatgtct gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata
     7201 attaattagt ataaaatatt aattaagagg aagtgtaaag tattgaagaa tataatacct
     7261 gatgtaaaac cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc
     7321 tcagtacttt tattatttat tataaaagat atagagaaat atgaaacttt attacatgcc
     7381 atatatacga tatttttagt agctacagga gtaattatat ttaatactac aaaatttaaa
     7441 gtagattcat tttctacatt tttaggatta atttttatag ctactggagt attggagtgt
     7501 atatatttat ttaattcctt ggggattaaa actaagtcta taatggagat taacatcaca
     7561 atatcagcaa taacagattt atttcctata ctgggtgtat acttaagttt taaatttgtt
     7621 aaggataaca aacaaattta ttcaagtgta attttattta tagcaactgc tacattaaca
     7681 ataacatctt tattcataat atcaggaatt tgtgactatc tgggaaatgg taaatcacaa
     7741 tatgctttag gagttataat atctatattc ataataatta tttctatcat tagtggaatt
     7801 gaattaaatt caagttcaaa taaaggtaaa tgggaatatg atgaaaaaaa gatttttaac
     7861 agaataatag taataataat cttatcaaga gttccaaatc tattgcatat agttattgat
     7921 aatagaaatg tagagaggat attatcacaa ataataataa atgtagcctt atattattta
     7981 tataattaca tagtatctaa gaatattaag aaaacagcac tggaattgca tgatacgaat
     8041 gaagaattaa caaaaaaaac ggaaagttta aaagaaaaga ataaaaaatt aatctatgag
     8101 acagaaaaga tagaggaact taagggaata cttatgaaaa gagaatctag gctacaatct
     8161 acattagatg tagcagtaaa tagcatagta gtatttagca aagatggaga aattacatat
     8221 gcaaataaga gctttagaaa tacttttgga gactataaag aacaagataa attgactgat
     8281 aaaataaaaa acttcaatga tttgatagaa agcattcatt atgtgtttgt tcatgagaag
     8341 aatgcagaga aacttattag tacaagcgat aataaagttt atcaagctat atttgcacct
     8401 ttaattattg cttcacaaac agaaggagta ttgtgtgtac tagaagataa gactaagaaa
     8461 aaggagtacg aacgcaaact tattgaagca aataagagaa gtaaagattt tttagaaagt
     8521 attggagatg gcatagtagt gcttgaaggt aataacaaaa tatatgttaa taatgcatgt
     8581 agggaaattt ttaaaaataa tttagagagt attgattttt cattagtatg taaaagtgaa
     8641 gaaagtatag aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt
     8701 tctcagtata ctaatgctgg tactaataaa actattattg taatacgtga tacaacctct
     8761 cgaaaaatat ctcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat
     8821 atattacctg atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag
     8881 tcgtttttag atatgctagc ttttacaaac atagatgata taaaagattc taatattaaa
     8941 ctattaatga atgcgaatac tgaagaaaaa atgaaattta cggataatat ggctaaagtt
     9001 ttaaatgaaa atagaagtat acttttactg gaacatgaat tgataaatag ttatgatgat
     9061 atagtagaag tagaattaag tgcattacct tttgctatat acaatactag atacataatg
     9121 cttattataa aagatttagt acataagaaa tattccgagc aagcagaaaa agaactgctt
     9181 gaaagattta aaacagataa aatcaaaacg gagttttttg caaatatgtc tcatgaacta
     9241 aaaactccac taaatgttat atctagttca aatcaactgg tagattcttt ctatagaaat
     9301 gaaaagataa aagattacaa taacaatatt aaatctcatg tggatttagt tagacaaagt
     9361 tcttataggc ttcaaagatt aataaataac attatagatt taacaaagat ggagtctggg
     9421 ttctatacac taaaacttgc gaaatacaat attgtatctg taatagaaga cttatttatg
     9481 aatatagaag agtatgcact tagaaaagac ataaaaattc tttttgatac agaccttgaa
     9541 gaaattaatg tttatataga caaggttgaa attgagagaa ttatgttgaa tctattgtca
     9601 aactgtatta agtttactga taatggaggc tggatatatg taagtattca ttataaaata
     9661 gataaagtaa tcataagtgt aaaagacact ggagttggaa taccacaaga taaattagag
     9721 ttgatttttg aagaattttc acaagttgat aagactttat ccagaaatac tgaaggaagt
     9781 ggaatagggc ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaagta
     9841 gtaagtgaag tgaataaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt
     9901 agtgaacatt acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa
     9961 ttttctgata tatattatta aaaatgatta tgagcaaaaa taaaataaaa atactatttg
    10021 tttaaatatt taagttgtaa gaaataggct atcataaggt aaattattat gatagcctat
    10081 ttttagctag gttttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac
    10141 taaaatctcc aaagtcgttt ggcagtataa aatgcatacc cttttttaaa ttatattcta
    10201 cagaattgtg tataagtttt ccatttccat ccaacacact gtataaagaa aaaggtgtat
    10261 tgtgtgtaaa attacatttt ccaaaaacat ctaatttata aacactaaaa aattcgtttg
    10321 acacaaatgt tgtgcattta aagtctgatg ttgatacgat tttatagtca gtatcgaaat
    10381 ttatatgagg aacattcgta acatctattg atttttgaac atgaagttca cgcttattac
    10441 ctgaattgtc agttctatca taatcataga ctctataagt agtatctgag ttttgttgag
    10501 tttctaaaat caatgttcct ttgcatatag catgtatagt gccacttgga acatagaaaa
    10561 agtctccctt ttttatatta acctttctaa gtagtttgtc ccattcatta ttatttacca
    10621 tatttacaaa ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag
    10681 aacaatctat tacataccaa cactctgtct tacctaactc tccattttca ttttttttag
    10741 catattcatc atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg
    10801 taagtaaagg aaacttatca ccaggtgtgt ttccaaataa ttctctattt ttattccaca
    10861 attcagatag ttttttacct ttatattttc cattttctat taagcaatct ccatttttat
    10921 gagagcttat agcccaacat tcaccagttg ttggcgaatc tatttcataa ttaaatttat
    10981 ctttaagtgc agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt
    11041 ccataatcct gttcctccta tgatattaaa tttatcctac aaataattat atataaatag
    11101 attataaaat caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt
    11161 attaaataag ataaactatg atatataata taaagttcta aattggagtg ataattatgg
    11221 agttgtttaa ggcagaaagc aaaaaatata aaaaagaatt tttaaaatta ttggagcaat
    11281 gggttagtat accttcattt tatgatagaa aaactgtatc taaagacatg ccttttggaa
    11341 aaggagtata tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag
    11401 taaaaaatat agataatcat gctgttcaaa ttgaatatgg aaatggtaaa gagtatgtag
    11461 atatatttgg acattgtgat gttgtaaatc caggtgaagg atgggatagt gaaccattta
    11521 aactaaatat tataggtgat aaattagttg ctagaggtgt atctgataat aaagggccta
    11581 tgatagtgaa ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagaa
    11641 aggttagatt aatagctggg ggaaatgaag agagtggatt taaatgtata aagcattatt
    11701 atagcaaaga gccatatggt gtatgtggat ttactccaga tgctaaattt cctgtattaa
    11761 atggagaaaa aggtggagca ataataaagt taatttcaaa tatagacgat aaatctttat
    11821 atataagtgg tggaatagag tttaatacaa taccagataa agtttatata aagaatgtag
    11881 aaaaattggg taaagataat atatgttttg atattaacaa tatatcgata aattatgata
    11941 atggcaatta tattgtgtat ggaaaaggtg gtcattcatc taaacccgaa aaatcaataa
    12001 atcctatatt agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag
    12061 atttatataa gttgataaat caagataata taaatggaaa cttatttggt ttaaatattg
    12121 aggggaaatg tggaatttta tcaatggtgc caacaataat aaatatagta gatggaaaat
    12181 tagaagtagt tttaagcgtt agatatccag aaatattaac cattgaagat ataataaaaa
    12241 aatttaattt gtatatggag caaaataata ttaataaatt tgagcttatt ggggaaaatt
    12301 taaagcaagc aaattatata gatagaaatt caaaacttgt aagaagtttg catgatatat
    12361 atataaagta ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg
    12421 cttctgaaat gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggca
    12481 atgtccatag tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat
    12541 atgcagaggc aataattaca ttatgtaata aaatataaat acaaaatgtc ccttatatag
    12601 acatattata tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg
    12661 atacaatgat aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat
    12721 atatgacatc aaacgaatta gctatcaaat tggatgtgtc tactaaaact attaaaagat
    12781 atatagcaga tttaaatagt gttttaagca aatatgattt ggtaatagct tcttctagag
    12841 gtataggata caagttaagc ggttctaaaa acaatatagc cagagctgtt aaagaagcaa
    12901 ataaatatat tgatggtttt ttagatgact ctgaagagtc aagaatgtca aatataatat
    12961 gtatgcttat taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa
    13021 gtatagccgc tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact
    13081 tagttataaa atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa
    13141 ggcaattaat tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt
    13201 tagatgacat tagtgaaaac gatatacact gtatagaaag tattttagaa aagcacttaa
    13261 gagaaaaaga tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg
    13321 tttctgtatt taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt
    13381 atagatttca taactactac tttatagaaa atctaatgaa agaaatatct gataaaattg
    13441 gatttaaact tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct
    13501 ataaaggtac acaaggaaga aaaaacacaa gtgaaattga ggaacgaata agtagtgtta
    13561 taagtagtgc acttcaagat atattcctta tttcaggtag tgattataca aaagatgatg
    13621 agtttatggt tgcaatctct gaccatataa agagattttt aaatagagct agagctaatg
    13681 taaaatcaaa taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt
    13741 tagccgtgtt tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg
    13801 agcttggata tatagctatc cactttgctg cttcaaatga aagaatgaaa aaaaatacta
    13861 gtaagaaaat ttgtatagtt tgtcattatg ggataggaac aggacaactt atttctgaaa
    13921 agctaaaaca gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatctcg
    13981 atatggctat aagtcaagat gttgatttga tagtatctac cgttgaatta aaaggatatg
    14041 aaaaacctgt cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca
    14101 aagcctttta tgaaaaggaa gaaagaagaa agataataag caacatgttt gatgaaaaag
    14161 cttttttcag tataaaggct aatactaaag aagaggtcat aatttcactt tcaaataaac
    14221 taaaagaaag agattttata gaggaaagtt ctataaaaag tataatagat agagaaaata
    14281 tttcatcaac tgaaatagga aatctagtag caatacccca cacaatagtg aagggagata
    14341 aaaaatctat aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg
    14401 ttcagctagt atttatggta ttttttaata ctaaagaaaa acataatttc tcgatattta
    14461 aataccttta taattttata aaagatgaag gcggagtaag aggaattatt aaaatatgtg
    14521 actttaataa attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa
    14581 catatttaac aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt
    14641 aaaatttata tcaaaaaaag ctaaagactt aaacttggca gaagatgaga gcttagtata
    14701 tgaaggttta atggctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat
    14761 acctcacact aaaaatgatg caattgaaaa tccagcagta gtagtattga agtttaatga
    14821 agatgttgtt tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc
    14881 aggaaaaagt aaagagaata ttcatcttaa attattatca tctctttcaa gaaaattaat
    14941 aaataaagaa tttaaagatt ctcttttaaa aagtgataat gttgaagaaa tatctaactt
    15001 aataaatgaa gctttaggtt tataattaaa cactaaggtt ataaaataaa aaaataatta
    15061 actaaaaata ggaggaattt gaaatgaata aaaaattagt agcattatgt gcttgtccaa
    15121 tgggactagc tcacactttc atggcagcag aagcaataga gcaagcagca aaggcattag
    15181 gttatgaagc aaaagttgaa actcaaggtg ctgatggtgt acaaaatgag ttaacaagag
    15241 atgatatatt aggagcaact atgataatac atgcagtagc aataacacca gaaggtatgg
    15301 aaagatttga tggttgtgaa gtttatgaag ttgaattaca agaagctata aaaaatgcag
    15361 aaggtgttat aaaagaaata gaagaagatt taggaatata ataaatttat tgtggaggaa
    15421 atagaatggc aattaagaaa aaagttataa acagttcagg tgcttctaca acaggaggaa
    15481 ataatgtagg aaaatctact ccatctagtc caactcaatc aaagggaaat ggaaaatgga
    15541 aagaaataag taaacatata atgacaggta tatcatatat gataccagtt ttagtaatgg
    15601 gtggacttat aggggcctta tctcaattaa taccatatgc tatattagga cttgacccat
    15661 ctgtaggtat agttgatgct atgaactcag gagaatttac agggttcaag cttagtcttt
    15721 taaatatagc acaattaatg tcaaatttcg gatttacttt gtttggattt gcgataccac
    15781 tgtttgcagc attttgtgca aactctatag gtggaaaaac agctttaatt gcaggattta
    15841 taggtggata tatagctaat aaacctgtag gtgtaccaca attcgttgat ggacaatgga
    15901 ctgaagttgt tccagttgca tcaggatttt taggtgcaat actaatagca tttataatag
    15961 gatattttgt aaaatggtta aataaatcta taaaagtttc tcataactgg ttagctttta
    16021 aaacaacatt tttaatacca ttaatagcgt cattagcttg tatggtattg atgatattta
    16081 taataactcc atttggtgga ttaattaatg aaagtatgaa aaacttctta acagcagcag
    16141 gagcagctgg agaatatgta tatgcaacag ctttggcagc agctacagca ttcgacttag
    16201 gaggaccaat aaataaagca gcaggattcg ttgcacttgg tttaactaca gagaatgtac
    16261 ttccaataac agcaagaaca atagctatag ttgttcctcc aattggatta gggttaacaa
    16321 cattattaga taaaagatta gtaggaagaa gagtatatga tagacaattc tatcaagcag
    16381 gaaaaacttc tatattctta tcatttatgg gtatatctga aggagcaata ccatttgcac
    16441 ttgagagacc aggttttgtt atacctttaa acatagttgg aagtgtaata ggagctatta
    16501 caggtataat tctaggagca attcaatggt tcccagagtc agctatatgg gcatggcctc
    16561 tagtagataa cttatttgga tatataatag gtatagcagt tggtgctata ttcatagcag
    16621 taggaaatat attctataga aataaattaa taaaagatgg taaacttgtt gtagattata
    16681 tcgactagta tttaattttt actatctata aataactttg gctggtaata tggttttaat
    16741 caatattacc agccttacct atatgattaa ttaaaggcgg tggagataag atatggataa
    16801 gatagaaaaa gtaagagagt attttagaga atatgatata gatggatttc ttataaatag
    16861 tcctacaaac aagttttatg ttggaaattt atttagctct tcaggttatg tttttataac
    16921 gaaagaatca caatatataa tagtagattt tagatatttt gaagaaatta aaagaaaatc
    16981 tagtttgttt aatgtagtat taatggacaa aactagaact cattttgata taattaatga
    17041 tatttgtaga gagcagaata taaaggaaat tggatttgag ggaaatgaag tatcttttga
    17101 tttatataga agtatgagta ataagttaag tgcaacctta aaatcagttg atttatctac
    17161 attaagggaa acaaaaaatg aagacgaaat aaaatatata aaaaaagctt gtgaaatagt
    17221 tgatgctaca ttttatcata tagtagactt tataaaggtt ggtatgactg aaaagcaagt
    17281 tgaaaatgaa attgttagaa ttataaaaga tttaggtggt caaaaagaat ctttcgatac
    17341 aatagttgca tctgggctta gaggagcatt acctcatgga aaagcaagtg aaaaagttat
    17401 agagtatgga gattttgtta cctttgactt tggtgctaag tataataatt attgttcaga
    17461 tattaccaga accatttgta tgggtactat aaataaagag ttagaagaaa tatataatat
    17521 agtaagaaaa gctaatgaag agtgtataag ggttttaaga ccgggaatga ctactggaga
    17581 gatagataaa gttgcaagag acataattgg ttcatatgga tatgctggta attttggtca
    17641 taatctgggt catggtgtag gaattatggt tcatgaatat cctgcattag ctcctgaatc
    17701 aaatgaagta ttaaaagaag gaatgattgt aactatagag cctggtatat atgttccaag
    17761 tcttggaggc gtaagaatag aagatgatgt acttataaca caagatggat gtatgagatt
    17821 gactacatct actaaggatt taatagttgt aagctaaaaa taaattagat aaatatttta
    17881 taacaataag aaaagaagag gtaaatttaa atgtataatt ttgatgataa accagataga
    17941 gtaagtgaaa agtgtagaaa atgggattta aatattataa gagataaatt tggagatatt
    18001 agagaagatt ttataccaat gtggattgct gatatggatt ttaaaatacc aacagaaata
    18061 gaaaacaaat ttattgaagc tgtaagaaga ggtgtatttg gatatacata ctgttatgat
    18121 gaattttatg attcagttat tggttggcaa aaaaatatgc atgaggtaaa tgtagaaaaa
    18181 gaatggatta cattaactta tggaactgta tcaacacttc attatgtagt acaagctttt
    18241 tgtaagaaag gtgatagtat aatattaaat acaccagttt atgacccctt tgaatcatca
    18301 gcaaaaaaac aaggtgtaaa tgtaatttgt aatacattag atgttgtaaa tgatagatat
    18361 tatataaatt ttgataaatt agaagttcaa attaaagaaa ataagcctaa attaatgatg
    18421 ttttgtacac cacataatcc atctggaagg atatggacaa ttgaagaaat gacaagggta
    18481 gcaactatat gtaaagaaaa taatgttatt ttggttgcag atgaagtaca tgcagagcat
    18541 attcattatg gtacatttaa ttcaatactt aagattggaa aagagctttt agaaaatgtt
    18601 attctattga catcaccaaa taaaggattt aatcttggtg gattaaagac ttcttattca
    18661 atagttataa ataaggatat aagggacaaa tttagaaata aattaaagca aaattctata
    18721 acttctccaa atgtgtttgg aataatagga ttgataaccg cttataatga atgtcatgag
    18781 tggttaagtg gtgtaaatga atatataaaa tcaaattatg aacttttaga aacttgggta
    18841 aataaatata ataagataaa attgatgaaa atggaatcat cttacttagc ttggatggat
    18901 ataagtggat taggtattag tgcaagtgag tttacagata aattggctat agatacagga
    18961 gtattattag aagatgggag tcattttgta aaagatggtg agaagtatgt tagaataaat
    19021 ttaggaactc aaaaggaaaa tgtaattgaa gctttaaata gaatggattt atttttaaag
    19081 tctttataaa aatttggttg aattagtatt ttatataaat ttaaactatt aatatggtat
    19141 atacataagt agaatttgtt ttagattact taagtatata ccatatttta tataaaaatt
    19201 aaatttgcat tagtcatgtg ataactattt aaattcatat gtattttgta tgtataatag
    19261 atataattat tatttaaact tattgtttat ttaatttttc aatagaaaaa gatttatctg
    19321 taagtttaaa attatctttt atacccttag ttaagtatac tttcttatcc ttagttatga
    19381 atatagcatc tacattatca attttattaa ttaaattcat acctttttca agaccaagac
    19441 caaatgtaga agtggataag gcatcacaat taatagattt atcagagatt attgtaacac
    19501 tacttaaatt gttttcaaaa ggataccctg taaatggatt taacatatga tgatatattt
    19561 ttccgttttt ttcgataaat ctttcataaa ttccagaagt aacaacagac ttatttgtag
    19621 tttctatgtt tcctatggat tcaccatttt tactagttgg gtcttgaata cctattttaa
    19681 aaggtttatc atttttacca ttttctaaag taaatatatt tcctcctaag tttactaaac
    19741 ctttttttac gtcctctgat tttaaatagg ctacaatttt atcagcagca tatcctttag
    19801 ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat ttattttttt
    19861 catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc tcagaatcac
    19921 ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga ccaattgtta
    19981 tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg acgaaaaatg
    20041 tatcatcaga aactctgaca aattttttac cagcattatc atttatttta gaaacatcac
    20101 ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa tcacattcat
    20161 ttaagatttt atcacttttg gattctttta cattaaatac agtaacttcg tttactgtac
    20221 caaggtagta gctagtttta ctatatttag tattctggct tgaactccta aaaaatgaaa
    20281 ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc ttattcatgg
    20341 tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca taggaggaca
    20401 aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta ttacagcatg
    20461 ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga tttatgtaga
    20521 taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa tagagaatag
    20581 aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag atgcttcatg
    20641 tcctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa gcatagtttg
    20701 tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata atcaagaaga
    20761 tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa ttatggttaa
    20821 cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt actacttaaa
    20881 cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa atatgcacaa
    20941 tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa tattaggaca
    21001 agacccttat catggagata atcaagctca tggtttagct ttttcagttc aaccacaagt
    21061 gaaaactcca ccatctttat taaatatgta taaagaatta aaagatgatt taggttgttt
    21121 tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac ttttaaatac
    21181 tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat gggaaatttt
    21241 cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt ttgtactatg
    21301 gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt atattttgga
    21361 agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta aacacttctc
    21421 taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc agatagaaaa
    21481 tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat gacattgttg
    21541 taactaatag aaatatgctt aaatagttga aaatactatg tatgcatatt tctatttttt
    21601 tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa aacacaaata
    21661 aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta aaaaatctta
    21721 gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc ggtaatggca
    21781 gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca agtaacatta
    21841 gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg agacctttta
    21901 gaatctcaaa acattagcta tgatgcaaat gatatagtaa ctttagatga aaatacaaag
    21961 ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt tgtagaaact
    22021 aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa gggtgattct
    22081 aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga aacttatcac
    22141 aatggaaaga aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga gccagtaaat
    22201 aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag gggagaaagt
    22261 tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg taaaaatatg
    22321 agagtggtag caactgcata tactggtcac agtataactg caacaggaac aaaacctaag
    22381 tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt ttatatacct
    22441 caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg aaataaaatt
    22501 gatatctaca tgaatgattc aaaaacagtt tataactggg gaagaaaaac tatagatata
    22561 cgtatattaa aatagttaaa taatttatat ttttactgga aattatattt tgattatata
    22621 gagaagaaac tctttataga ttctaaaaat ataatttcca gttttttatt ttggatatgt
    22681 aataaaacag gtaaatttta accatgtata gataaaagct atttaaatta taatttacta
    22741 tcatataaat ctataaaaat tgttgcaaag agtcaaaata tatgatatta tagatttcgt
    22801 tgagaaggat ttcattatgg gagtggatgg gtgccggtgt gccctctagt cttcaaaact
    22861 agtatgaggg gttaagagcc tcttaggtgg gttcgattcc cacgcattcc cgccaatatg
    22921 taaaaggtat cttttaatta aaagatacct ttttaaattt gttttaagat aatttagttt
    22981 taatgatata taatattttg tattatataa tgtaaatttt ataatactat tattgttcag
    23041 ataaaagtct aggagagtga aaagatggaa aataatttta caacgagaac tagttttcta
    23101 gttggagatg atgggataga aaaacttaat aattcaaata taatagtatt tggagttgga
    23161 ggagttggta gttttactgt tgaagcttta gcaagagctg gagtaggcaa tattactatt
    23221 gtagattttg atgatgtaga tataaccaat ataaatagac aaataccagc cctacattca
    23281 actgtaggaa gatacaaagt tgatgtcatg gaagaaagaa tattagatat aaatcctaat
    23341 atcaatataa aaaaaataag aagtctttat aataaagata ctagtgacga aatactaact
    23401 gaaagatatg attatgttgt ggatgcgata gacatggtaa gttccaaaat acatctaatt
    23461 gaaacttgtg aaaaaaaagg attgaaaata ataagttcta tgggtatggg aaataaatta
    23521 gacccaacta aaatagtcgt tacagatata cacaaaacaa gtacttgtcc tctagctaaa
    23581 gttatgagga aagaacttag ggatagaggt atcaaaaagt taaaagtagt atattctact
    23641 gaacaaccaa tagaacttaa gaagaaagta atgaatggta gaaaagtaac acctggtagt
    23701 gtatcatttg tcccatctgt aggaggttta ataatcgcat ctgtaatagt aaatgaacta
    23761 ttaggacaat aagttttaaa tattttaatt tataaatttt atatacaaat ttgaaggcta
    23821 gaaggatgaa tataaagcaa ttttatattc atacttttag cctagttatt ttatattacc
    23881 ttattttatg tagttgtagc tttatttttt agattatgta tctatttcat aattatattt
    23941 tgtaaatata atttctccat cactataaaa aacaatatct cctattctgt ctgttctata
    24001 aattgataca tggttttgtt ttagattatt aataacctcc ctatgtggat gaccatattg
    24061 atttttatat ccacatgata ttgcagctat atctggacta atttttttta taaattctaa
    24121 agatgaagaa gaattacttc catgatgacc tatttttata aaattaatat tatttaattt
    24181 aaaagaatgt aaaatttctt tttcattttc ttcttcagca tcacccataa ataaaaaaga
    24241 attatcatta aaagttaatt tgaaaactat agaatttaaa ttagattctt cttgtatata
    24301 tgatggactt aatacatata tatttatatt gttatctatg tttaaaacat catttttata
    24361 gagatgctga atagataagt ttttatccgt acaagaattt attagatttt gataagcctc
    24421 actatcagtt gattgttcag gcatatatat ggagtttaca ttaaattttt ttataatatt
    24481 atcaagactt cctatgtggt ctgaatctgg atgagttgct attataatat caatagtttt
    24541 tattcttttt tgtctaagat aactaataat tatattttcg ctatcttcat cacctccatc
    24601 aattaatata ttcttatttg tgggtgtttg aactaaaata ctatcacctt gtccaacatc
    24661 aatcatatgt atagatagta gagatttttt gtcacagccg attaaaagtg atattataat
    24721 aagtattgat aaaaaaattt tatttctcaa taaaaaatca ctccattcat tacatgttta
    24781 tataaattta tgatgggtaa tataagatat atatgactta ttatatttta tattttaaac
    24841 aatataaatg aaattaatac cataataaaa ttggagtttt ttatatatac aaaataaaat
    24901 ttttaaaatt aagcaataaa aaaggtagga ggattttatg tttttcaaag atattgaact
    24961 aaattcaaaa aaagagttag acccatattt tgatttagta gattatgaag cttgtgagta
    25021 ctgttttagt actttatata tgtggcaaca tgtttataag acaggatatt atataggtga
    25081 agattttgcc gttttagttg gggaatacga aggagatagc ttctcaatat taccacttgc
    25141 taaaaaagat aaacttccag aagttgtaga ttttgtcctt gaatattttt ctaaaaataa
    25201 taaaaaaata tacttaagag gtattactac tgaagtagta gagtttttaa aagaaaaata
    25261 tccaggtaga tttgagtata tagaagaaag agatttattt gactatattt atgatgcaga
    25321 aagtttaaga actctagctg gtaaaaagaa tcaaaagaag agaaaccata taaactattt
    25381 cttaaaagag tatgcaggta gatatgaagc taaattatta gataaagaaa actttgatga
    25441 gtgtttagta ctaatgaagg aatgggaaag taataaggaa gaaaataatg aatttgatga
    25501 aagtatggat gatgaattaa taggtatcaa aaagatattt aaccattatg acatattaaa
    25561 agataaggta aaagtgtttg gtgtttatgt agatggaaaa ttagaagctt ttagtatagg
    25621 agagctttta aatccaaata tggcattgat tcatatagaa aaagctaatc ctgatataag
    25681 aggtctgtat ccatttataa atcaacaatt tttggtaagt gaatttaagg acgttgagtt
    25741 tgtaaataga gaagaagatt taggtattga aggattaaga aaagctaaac tttcttatca
    25801 tccttgcaga tttgttgaaa agtacagtgt tagggaggct taatagacta tatggagatt
    25861 aggtatgcta aagaagaaga gatagaaagt ataaaagaga tttggagtta ttgttttaac
    25921 gatacggaga gttttatgaa gtactatttt aatgataaat ataaatctga aaatactgta
    25981 gtagctttag atgaaggaaa aattatttct tctcttcagc taaatcagta taagttatta
    26041 ctaaatagca aggtatataa tacatcatat gtagtaggag tttctacact tccagaggga
    26101 agaggagctg gatatatgaa caaagtaatg aaatttactt taaacgaatt gtataaaaaa
    26161 gggcaattag tttctatatt gatgcctatt gactatagac tttatagaag atttggatat
    26221 gagcattgtt acgaccagat tgaatatact attaacacag atgatttaaa aaattttaag
    26281 tcaagtggaa aaatgataaa gtctaattta tcacaaattg atgatttgat acaaatagat
    26341 agaacttttt taaatgaggt taatggtaat gtgttaaaag atgaacatta ttatgaaaac
    26401 ttatttaaag agattcaaag tgaagatgga tttttataca ttcatgaagg aaatgaaaaa
    26461 gatggatata tagtttattt tcttcaggaa gataaaatgt ttgtcagaga gttattttat
    26521 aaaaatatag atgcccttaa atcaatgctt aaatttatat ataatcacaa tactcaatgt
    26581 aagatagtta ctatatctac tcctacaata gataagataa gatttatatt agacaaccct
    26641 aaagatagtg atataaaaat aaaacctttt atgatgggaa gagttataaa tgtaaagaag
    26701 tttattgaag atatagatat tgagaatgat attaatagct catttaattt attaatagaa
    26761 gacaagttta tagatgaaaa taatggtcta tttaaaattt ctatacagaa taagaaggta
    26821 tctgtagagc agttagataa aaaaggtgca gaaaagcctc aagaagattt tgatattaaa
    26881 ttagatataa ataccttaac acagttatca ttttcttata tagatgtaaa tgaagctatt
    26941 ttcttaaatg atataaaaga tgtttcagaa gaaacactag aaacacttaa ttgtattttt
    27001 tctaaaaaaa ataacta
//
LOCUS       DAYASV010000044        26157 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYASV010000044 DAYASV010000000
VERSION     DAYASV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26157
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.044"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            142..759
                     /locus_tag="ACGI68_001804"
     CDS             142..759
                     /locus_tag="ACGI68_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HFH0138245.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(916..1368)
                     /locus_tag="ACGI68_001805"
     CDS             complement(916..1368)
                     /locus_tag="ACGI68_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staygreen family protein"
                     /protein_id="HFH0138246.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1816..3174
                     /locus_tag="ACGI68_001806"
     CDS             1816..3174
                     /locus_tag="ACGI68_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0138247.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDSEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3447..3827
                     /locus_tag="ACGI68_001807"
     CDS             3447..3827
                     /locus_tag="ACGI68_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0138248.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3952..4152
                     /locus_tag="ACGI68_001808"
     CDS             3952..4152
                     /locus_tag="ACGI68_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138249.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4348..5076
                     /locus_tag="ACGI68_001809"
     CDS             4348..5076
                     /locus_tag="ACGI68_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138250.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5098..6015
                     /locus_tag="ACGI68_001810"
     CDS             5098..6015
                     /locus_tag="ACGI68_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HFH0138251.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6157..7341
                     /locus_tag="ACGI68_001811"
     CDS             6157..7341
                     /locus_tag="ACGI68_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0138252.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTYLDVINFDKVESIVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7448..7636
                     /locus_tag="ACGI68_001812"
     CDS             7448..7636
                     /locus_tag="ACGI68_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFH0138253.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7785..8435
                     /locus_tag="ACGI68_001813"
     CDS             7785..8435
                     /locus_tag="ACGI68_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0138254.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            8538..9476
                     /locus_tag="ACGI68_001814"
     CDS             8538..9476
                     /locus_tag="ACGI68_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0138255.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9598..10353
                     /locus_tag="ACGI68_001815"
     CDS             9598..10353
                     /locus_tag="ACGI68_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0138256.1"
                     /translation="MKKYNISENGALIKNDSIESKSESVKVVQISDTQIGSFYSTKNL
                     KKVANKINTLNPDIIVFTGDLIDYSNKNPSVDEITTILSSMNARLGKFSVFGNHDYMY
                     KLPRYYRQIMKNSNFNLLVNENKKIKLKDDKYINILGVDEILNGNPNIKYLESQIDNK
                     SFNLLLAHEPDLVDMLSKDTMNLVLSGHSHGGQIRLPIKGALVTPPYGRKYTKGFYDI
                     NGNHLYVSSGLGSTKLPFRFFNIPEIIEFNIII"
     gene            complement(10638..11372)
                     /locus_tag="ACGI68_001816"
     CDS             complement(10638..11372)
                     /locus_tag="ACGI68_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFH0138257.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            11497..12213
                     /locus_tag="ACGI68_001817"
     CDS             11497..12213
                     /locus_tag="ACGI68_001817"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0138258.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            12347..13039
                     /locus_tag="ACGI68_001818"
     CDS             12347..13039
                     /locus_tag="ACGI68_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138259.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            13029..14210
                     /locus_tag="ACGI68_001819"
     CDS             13029..14210
                     /locus_tag="ACGI68_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0138260.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSMISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            14492..14947
                     /locus_tag="ACGI68_001820"
     CDS             14492..14947
                     /locus_tag="ACGI68_001820"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFH0138261.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            14937..15662
                     /locus_tag="ACGI68_001821"
     CDS             14937..15662
                     /locus_tag="ACGI68_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HFH0138262.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIKINKDNVVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            15634..18015
                     /locus_tag="ACGI68_001822"
     CDS             15634..18015
                     /locus_tag="ACGI68_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HFH0138263.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(18220..19449)
                     /locus_tag="ACGI68_001823"
     CDS             complement(18220..19449)
                     /locus_tag="ACGI68_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0138264.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            20050..20964
                     /locus_tag="ACGI68_001824"
     CDS             20050..20964
                     /locus_tag="ACGI68_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0138265.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            20988..21623
                     /locus_tag="ACGI68_001825"
     CDS             20988..21623
                     /locus_tag="ACGI68_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HFH0138266.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            21999..22715
                     /gene="pyrF"
                     /locus_tag="ACGI68_001826"
     CDS             21999..22715
                     /gene="pyrF"
                     /locus_tag="ACGI68_001826"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFH0138267.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            22741..23784
                     /gene="carA"
                     /locus_tag="ACGI68_001827"
     CDS             22741..23784
                     /gene="carA"
                     /locus_tag="ACGI68_001827"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFH0138268.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            23837..>26157
                     /gene="carB"
                     /locus_tag="ACGI68_001828"
     CDS             23837..>26157
                     /gene="carB"
                     /locus_tag="ACGI68_001828"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HFH0138269.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
BASE COUNT        10585 a         2723 c         4668 g         8181 t
ORIGIN      
        1 ttttaaggat ataaagtaaa aaaatataca atatcaagaa aaaaagatat tgggtattta
       61 aagtaatact ttatttaatt ttaagtaaat cgggtatcta catatataga tactaatttc
      121 aaagaatagg agggtgttta tatggaaata ttcgcaatag gacattcaaa ttatccatat
      181 gataagctta ttaatatgat aaaaaaatat gacataaatt gtgtagttga cattagagaa
      241 actccatatt ctaaatataa tattcaatac aataaagagg catttaacga atctcttaga
      301 aattccggat ttttatatat atacatggga aaagaatttg gagctaaaag aactaataaa
      361 gatgtatata cgcaagaagg ttatgcagat tttgagaaag tagcaaaaga agatattttt
      421 ttaaacggaa tagaaagatt aaaaaagggt tgtcaaatgg gttataggat agttttatta
      481 ggagctatgc aagaacctat aagatgtcat agaagtatct tggtgggaaa agtattaaat
      541 aaagaaggtt ttgatgtaaa atatataatg catgaaggaa atttggcata tcaggaagat
      601 atagaggaat cattattgga taagtatttt agtgatagaa agcaactgag tattgacaat
      661 ttattaggga gtgcattaac gagagaagaa atgatacaag aaggttataa gctagctaat
      721 aaagaaatag gatatagaac tgagaaacta ggtaaataaa aatatacaaa tagataaaat
      781 acagtactta tcttaagtaa aataatatta tgaatttata atagctaggc atgatgtgcc
      841 aattaaaaat attctagtta tttaataata tcattaaatt tagggtattt tttaatagca
      901 catcatgttg cagttttaaa aaataaaatc tctaatattg ccataatcaa ctgttctatt
      961 aagagtagga tgactagata taaatctaac tgtaattttt gaatttaaca aacaaggatt
     1021 tgaaattaaa aatactctat ctccattgac aatagaagta atagctactg gcaaaatatc
     1081 tacaaatgta tcatatctgt ttttagcaat ttgataagga taatttccta caaaaacaaa
     1141 tagatttaaa tcatatatat tatcagtaaa ccaagcccat tgaccataaa ctttgtcaat
     1201 attcattgtg cctaaaccaa gtgtatcaaa gtatgttgct atagttaaat aaatattttc
     1261 catgttttct aaatatgaaa cagtatatct tctgaaacgt atgggtgctg tggaagaaat
     1321 aggataaacc atatttacat ttagattatt tgcatttaaa ttaaccaaag tatcacatcc
     1381 taaaattagt ctataaaata caatatttaa atggatgcga ttttgttaca tctaaagtgt
     1441 aatattttat atattgaaaa agtggtaaaa gtttggatga ttaaataata ttataaaaaa
     1501 tatataaaat gtacttattg ttattgctac acgatagtga tacaatatta acgaaatata
     1561 gctactttta ataataacgg aaaatattta tattaaatta ataaatattt taaatgtggt
     1621 atccataaaa aataataaac ataagataat tataaagata tactgtgttc aaatgatatt
     1681 aaacaagatt tatattattt gaaagcagta tattataatg cgaacaatac aattctaaac
     1741 tttgcattgt caatttacag ttcgataaac taattaaaaa ttaaatttat ataaagaggg
     1801 aaaagaggaa tagggatgtt tataaggaaa aatgttataa agttagcaat tcctataatg
     1861 atagagcaga cgtttgtcat gcttttagga gtatttaata caatgatggc aggtcatatt
     1921 ggggaagaag ctgtatctgc agttggaatg gttgattcaa taaacaatat atttatatct
     1981 gtatttgctg cactatctgt tggagctact gttgttgtag ctcaacaaat aggaaaaaaa
     2041 gatagtgaaa aggtgaatga aactattaag caggctctag tgtctggagt aatattatcc
     2101 tttacaataa caatattgat gtgggttttt agaactggtg taattaattt attttatggt
     2161 tccgcagaag aattggtaaa atcaaatgca aaattataca tagagtttac attatttacg
     2221 tatccattta ttgcgacaca acaaatagca aatggtattt taagagggtg tggagatgta
     2281 aagaaaccta tgtatgtgac tatgtttatg aatatagtaa atgtaacatt gggatatata
     2341 cttatatatg gtattgattt aaatcctcta ggaataaatt tgcaaacacc atcctatggc
     2401 ataacaggtg cagctatatc tattgctata gctagaataa ttggatgtat agtaattata
     2461 agagctttat ttaaaggaaa cgaatttata tctatgggta aaatatttcc atttaagatt
     2521 gatgttgaaa ctcaaaagtc tatatttaat ataggaatac cagctggagt tgaacaacta
     2581 ctttttaatg caggtaaatt gattgttcaa gtatttatag ttactatggg aacatcagca
     2641 attgcatcca atgcaatagg aatgtcaatt gcatccataa taaatgtaat tgggaatgct
     2701 ttagctttgt ctgctacaac cttggtggga cagtatatag gacgtggaga tattaaagga
     2761 gctaagagta ctttattata tttaactaaa tttgcaacaa tttgtttatt tatagtagga
     2821 gctatattta taccaattgc accctggatt tcaagtatgt atactcaaaa tgctagtgta
     2881 attgacatct caacacaatt aattcgaagt gattgtttag ctatggtaat atggcctata
     2941 tcctttgtat tatcagcagg gctaaaggga gccggtgata ctagatatac aatgatgact
     3001 gcagtaatag gtatgtggat ttttagaatt tttacaggtt acgtattggg ggttgtatta
     3061 gaatttggag tgttaggaat ttggataggt atgtatactg attggcttgt tagagggact
     3121 ttatattgtt taagattaag aggaacaaag tggctaaaac acaaagttgc atagaatata
     3181 tgaaatcact tttagatgga taactctatt tagaagtgat tttttatgta gttattatca
     3241 taattataag tatgatatat aaggtgatta aattacataa ataaaggcta taatagtatg
     3301 gatattataa attatatatt gagcagaatt acataatttg gcgataaata ttagaaatat
     3361 atatctatag aaaataatat atatgggtat attattaaat ataaaaaata ttaggtattt
     3421 atttaataat aaaggtgggt attgttatga taaataaaat aggaaaaata actctctatg
     3481 taaataatca agaggatgca aagttatttt ggacagaaaa attgaacttt gtagtaactt
     3541 atgaaaagga aatgggtcca aatatgaaat gggtggaagt agcaccaagt aaaaatgaat
     3601 ttacaacatt tgtgttgtat gataaaaatc taatgatgaa gcaaaacaaa gagataaatg
     3661 tatctcatcc atcaataatt ttaagtacaa gagatattaa tggaacttat gaacaaatgg
     3721 caaataacga ggttgaggtt ggggaattaa tggagatgcc atatggaagg atgttttctt
     3781 ttaaagacca agatggaaat gattatttgg tcagagagga taaataattt tacaaagtat
     3841 taaatttact caatatctga tataattaga gacaataatt attataaact agtctaggat
     3901 tagaattata taattaaaaa ttaagtgtaa attttgagta gaggtgataa aatgtttaag
     3961 aaaatggcag tactaaaaga tatagcaact aaaataggtc gtaaaaaagc gtatgaacta
     4021 ttagaaatgg ttgaaggtaa tgatgccttt gtagctgagg taaagataaa aaagaatgga
     4081 atagaatcta aaaaagaaga aattatgtta aaagataatc aaaaaataat attagagtat
     4141 atagaaggtt aaattttaaa tacatataag ttgatatact aaatttctga gatgagttta
     4201 cataaaactc atctttttta cttttcaata actttgtttt attatttaat atacatttta
     4261 tctccttaga gttgctgata tataaaaatt actgatataa taaaagaatg gatattaatg
     4321 taatatttta tagaaaagag gggtattatg cttgaaataa aaaatctttc ctttaatgtt
     4381 gagtctaaca atgaggaact aggaataata aatgatgtta gcctttcatt tgagagggga
     4441 aaacttattg ttatcacagg tccaaatggt ggaggaaaat ctacaattgc taagcttatt
     4501 atgggaattg aaaaagctac gagtggtcag ataattttag atggagaaga tataacaaat
     4561 cttagtatta cagaaagagc aaaaaaagga ataggatacg catttcaaca accaccaaga
     4621 ataaaaggta tgacagttga aaatttactt acacttgctc atggaaaacc attgtctact
     4681 gatgtatgtt gccaatacct tacagatgtt ggattgtgtt caaaagacta tttaaatcgt
     4741 gaagttgata actctctttc tggaggggaa atgaaaagaa ttgagatagc aactcttttt
     4801 gctagagatt taaaggtttc tatatttgat gaacctgaag cagggataga cctttggagc
     4861 tttggaaaac tcaatgaaag ttttaagaaa atacatgaag agtctaatca gactataatc
     4921 ataatttctc accaagaaag aatattagag ttagctgatg aaataattgt tctacaagat
     4981 ggttcagtaa aaagccatgg aacaaaagag gctatattac ctgaaatcat gtgtcaagtt
     5041 aattcaagtt gtgaattaat gaaagacatg aattaattag aaaggaaggc aaattaaatg
     5101 aacaaaatag atttaaatct tttagaaaaa gttgctgaac ttcgtggaac tccattagga
     5161 gcatacaata tacgtaaaaa tgggcaagga gtagagagac attcaactga aaatgtaact
     5221 ataattccta aaactgataa acctggaata gatattatag taaagccaaa tacaaaagga
     5281 gaaaatgtac atataccagt aatagtaaca gaggctggtg tacaggatat ggtatataat
     5341 gattttgaaa ttggagaaaa tgcagatgtt gttataattg ctgggtgtgg tatacataac
     5401 tgtggttgtg atgatagtga acataatggt atacatagat ttttcttacg taaaggagct
     5461 cgactaaaat atgtagaaaa acattatggt gaaggtgatg gagaaggaaa gagaatttta
     5521 aatccagtta ctattgtcaa tatggatgag gattcatatt gtgaaatgga aacaattcaa
     5581 attaagggtg tagattcaac taaaagagaa atggatgcaa acctaggtaa aggagctaaa
     5641 atagttgtat ttgagaaatt attaactcat ggtaatcaag ttgcagaatc taatatgaag
     5701 gttatcttag agggtgaaaa ctcttctgct caagtagttt ctcgtacagt tgggcaagat
     5761 aattctaagc aaaagtttaa tccttgtgtt attggggatt ccctttgttc tgcacatgtt
     5821 caatgtgatt caataattat gggaaattct caaattagtg ctatacctga aattgctgca
     5881 aatcatccag atgctctttt agtacatgaa gcagctattg gaaagattgc aggagaccaa
     5941 atactaaaga tgatgacatt aggtcttact gaagaagaag ccgagcaaca aatacttaat
     6001 tgcttcttaa agtaaattaa agattttatt taatttaata taattaatat agtataatat
     6061 agtgaataag tctaaaatat ttttattttt aggcttattt tctttatgta agaaaagcta
     6121 ttagagaaat tatattgtaa ttaggatgtg gaataaatga aaaatttact tataagaaat
     6181 gggacaataa tatctccaac agatggtatc aaatttgaaa aaagagatat cttggtttta
     6241 catgggaaaa tatatgctat tggattgaac cttaaagaga aaataagaaa agaaaaatta
     6301 cttacaaata aagagttcga gataataaat gccgataata tgtatatttc cccaggattt
     6361 atagatgttc acacccattg ttataagaga aaattaccta caggtatgga ttctgattca
     6421 attggaattg aaaaaggttc tacagttata tttgatgcag gtacagctgg tccctcaaac
     6481 tactatgatt ttaaaaaaaa gtatatggat gagtgtaaaa cagaagtata ttctttttta
     6541 aatgttacaa atgagggact aaatatattg aatgaatcaa cttatttaga tgttataaat
     6601 tttgacaagg ttgagtcaat tgtagaaaaa aatatagaaa agataaaggg tataaaagta
     6661 aaagcgagta aatttcaatc taatgaaagt ggtatagata taattaagaa atcaaagcaa
     6721 gttgctaaaa aattaaattt acccttagtg gtatatatag gagagtatcc atcatatata
     6781 gatgaagtaa tgaatatttt gggaaaagga gatgtggtta caccggtata tggaaatagt
     6841 acaaatggaa tccttaaaga atctggcaca ttaagaaaat cagtaataaa tgcaaagaat
     6901 agaggagtat tgtttgatgt atgtcatgga gtggaccaat ttgattttaa agtttttaaa
     6961 aaagcaataa agcaaggttt agagcctgac ctaataagta ctgatatgca tataaaaaac
     7021 atgaaagacg taaactattc tcttttaaat gttataaata aaattatgga acttgggata
     7081 tccctagaaa agtgtataga aaaagtaact gaatacccaa caaagatttt cgatttacat
     7141 ggaaataaag gcagaataaa gattggtgga gaaggtgatt ttacaatatt tacaatggag
     7201 gagtgtaatg atattataaa agactacaat aataatgaac ttgttttaaa taaaaaatta
     7261 agattgcaat atactgtaaa atcatggatg aaaagcagtg aagtttatag acatggatac
     7321 gaaaatacta taataaacta aacaatagtg ataaaataat aaatatatgt ttaaattggt
     7381 atatattgga taaatatcta aggaaacaac aattaaaaaa taataatttt aggggaggta
     7441 ttttagtatg aaagcatttg ttgataagga tgtatgtgta gcttgtggag catgtatagg
     7501 aacatgtcca gacgttttta gtatgagcga tgaagggcat tcagtagcaa ttgaaggaga
     7561 aataccagca gatttacaag atttatgtaa agaagccgaa gaaggatgtc cagtttcagc
     7621 tataacagtt aaatagagct gtttaaattt atttttaaaa taaatatata agatttaatt
     7681 ggtacttaag ctatagtatt agaaatttct attactatag ctttttaaaa tagataaact
     7741 taatatataa gatattactt tttacaaata tggaggactg aaatatgaaa aaaaattatg
     7801 aaatagtatt attcgattta gatggaacac ttacagaccc taaagaagga ataacaaagt
     7861 ctattcagta ttctttaaat agctttggca taaaagaaga tttggaaaac ttagaccaat
     7921 ttataggacc accattacat gatactttca aggagtatta taaatttgaa gataaaaaag
     7981 ctaaagaagc tgttgaaaag tatagagaat attttgctga taaagggata tttgaaaata
     8041 aaatttatga gaatatgaaa gagatattag aaatgctcta taaaaatggt aaaatattat
     8101 tagtagcaac ttctaagcca acagtttttg cagaaactat acttagatat tttgatatag
     8161 atagatattt taaatatata gcaggtagca atcttgatgg tacaagagtg aataaaaatg
     8221 aagttataca atatgtacta gacctatgta atgtaaaaga taaagataaa gttattatgg
     8281 ttggagatag aaagtatgac ataattggag ctaaaaaaat aggtatagat tctattggag
     8341 tattgtatgg gtatggttct tttgaagaga ttagtgaaag tgaacctact tatattgtcg
     8401 aaaatgtaga gtctataaaa gatattttgt tataaatatc ttttatagac aaaaatatta
     8461 caaaatggtg tcgcatattt aaaaatatga tattatagtt aaggaagtca tttaaaaaag
     8521 aggaggatgc acttaaaatg aaatctattt taattatggg tggaagcgac ttcataggtt
     8581 ctgcacttgc taagcgcttg attaaatgtg ggtatcaaat tgatatactt acaaatggta
     8641 aaaaggaaat agattataat ggttttaaag aacatctaat ttgtgataga aaagttcgaa
     8701 aagatatgga aaacattatc acaggaagaa aatatgatta catatatgat atgacagcat
     8761 atacaaaaga agatgtaagt aatctgattg attttataag catggataac ttaaaaaagt
     8821 atatagtatt gtcagcagga gcagtatata aagatagtgg aagaaatata aaagaagaaa
     8881 atgagaaagg tgaaaatgaa aactggggaa aatatgggtt aaataaaaaa gaggctgaag
     8941 attttattat aaatagccct ataccatata taattattag accaacttac atatatggtg
     9001 aaaataataa tttatataga gagtattatt tctttgaaaa aatagaaaaa aatgaaaaga
     9061 taccagtacc aaaaggaaaa caagttagta atcaatttat atatattggg gatttggtaa
     9121 aggtattgga aagcataatg aaaaatcctc atgtaagaga ggcctataat gtgacaaatc
     9181 cacagttaat ttcttgggat gaccttatat atacatgtgg agaaattatt ggcaaagaac
     9241 ctataataaa atatgtagat atggaaaaag tagagtttag agaaagaacg tatttcccat
     9301 ttagaaatat agactttaat cttgatataa ataagcttat tgaacatggg ctttatatac
     9361 caaacgtgtt actaaaagaa ggtcttacag caacatataa atggttttca gcaaataagc
     9421 caaaaatgca tgacagaaaa atgaacaaag ttgagtctgt aatgcaaata gtttaaaaaa
     9481 ataaataagt aggtagatat tttgaactca aaaattaagt attttttaaa attttttatc
     9541 ttaatattta ttttgatttt tatatactca gttgcgatag agccgagtct attaattgtg
     9601 aaaaaatata atataagtga aaatggagca ttgataaaaa atgattcaat tgagagtaaa
     9661 tctgaaagtg taaaagtagt tcaaatttca gatactcaaa taggtagttt ttattcaact
     9721 aagaatctca aaaaagtagc taataagatt aacactttga atccagatat aatagtattt
     9781 actggtgatt taattgatta ctccaataaa aatccaagtg tagatgaaat tacaactatt
     9841 ttatcaagta tgaatgctag gttgggaaaa ttttcagtat ttggcaatca tgattatatg
     9901 tataaacttc ctaggtatta taggcagatt atgaaaaatt ccaacttcaa tcttttagta
     9961 aatgaaaata aaaaaataaa attaaaagat gataaatata ttaatatatt aggtgttgat
    10021 gaaatattaa atggaaatcc taatataaaa tatctggaga gtcaaataga taataaaagt
    10081 tttaatttat tgttagcaca tgaaccagat ttagtagata tgcttagtaa agatacaatg
    10141 aatttagttc tatctgggca tagtcatggt ggtcaaataa gattacctat taagggagca
    10201 ttagttactc ctccatatgg aaggaaatat actaaagggt tttatgacat aaatggaaat
    10261 catttatatg tgagttcggg tcttggaagt acaaagttac catttagatt ttttaatata
    10321 ccagagataa tagaatttaa tattataatc taacataaaa tatttagaaa atataatttt
    10381 gctttttata atatgtagat gtatatactg taaaaatgga ttaaaccagt agattgaatg
    10441 aattaataga ttaaattaat atataaaata tttaagttat aattaatgta ttagacaaaa
    10501 taaatactat taagaactat taaaatatag attaaagata ataacatata ataaataaaa
    10561 aagtaagatt gtgtagatta tataaagatt atttacaatt aatacaatct tgctttttta
    10621 tttaaaaatt ttaattatta tttaaaattt tttatcttct ctttatccca tataaacaat
    10681 ttctcagaat tatttaaagc accaaataca tttcttttaa aatcaggcat ctctttattt
    10741 aaattagcaa taagattttt tatgtactct ctcctagtat gaccatttgc agggcaggga
    10801 tttgtgataa taggatagtt agaatctttt gaaattttct tagtcatata ctcttctatg
    10861 taaaccatag gtcttataac tgttaaatcc tgtctgtcta ggtatgtctt tggggaaaaa
    10921 caatttactc taccctcata aaacatagac attaaaaatg tctcaattgc atcatcttta
    10981 tgatgaccaa gagctacttt attacatcca agttttttag cattatcgtt caaagcacct
    11041 cttcttaaat tagcacacaa tgcacaagga ttcttttctt gtcttatatc aaatacaatt
    11101 tcttttatgt cagtttgaaa ttcataaaaa ggaacattta tttctttaca caatttatct
    11161 agtggagaat tatcaacacc tccagtattt aatgttatgg ctatcaattc aaagttttgt
    11221 ggagaaaatc tttgataact tttaagtata tgaagtagag ttaaactatc ttttccacca
    11281 gaaagtccaa ccgcaatttt atcattttct tgtatcatat caaaatcttg tatagcctta
    11341 cgagctttac ttagaagttt ttgtatagac attttatcac catcctttaa aaatattaaa
    11401 tttaagattt atttaagatt tacttataaa atacttaagt cttatactct aaaattaaca
    11461 ttatagcaaa cataacaaaa aggagaagat ttaattatga aaaaatttaa attaatagga
    11521 gccattattt tagtagtatt tataagtgta tttatatcta aagatagatt acttaagaaa
    11581 gataaagatc caatagtagc tgctcaacat gattcagaac aagatgttga ggcaaattct
    11641 caaaataaaa atcactcaaa tagtatatca aatatattat tggtaaacaa gacaaatgga
    11701 attagcaaaa actatactcc tgaaaatatt actaaggtaa acataccttt tgttgaagaa
    11761 gcaacagagg aagaaaaaca aatggcagga gaacctgcaa aggcagttga agatttagtt
    11821 aagcaagcta attcagaagg aatacagttt ttagggtctt cagcatacag atcttatgat
    11881 actcaactgg atacttatac aagaagggta aaatctcagg ggcgagaaaa ggcagatgcg
    11941 tatgtagcaa agcctggtta cagtgagcac caaactggac tttgtattga tttaacaaat
    12001 ccagaaagat ggtttgttgg aagtacaaaa gaagctaaat ggttggcaga aaatgctcac
    12061 aagtttggat ttataataag atatcctgaa ggtaaagaag atataacagg tacagcatat
    12121 gaaccatggc atattagata tgttggtaag gatgctgccg aggaaattta ttcaaaagga
    12181 ttaactttag aagaatattt acaaaataaa taatataaat gtgtaaataa tttgtattat
    12241 attggaaatt tagaaattaa taaatttata gattacacta actaaaatat agtataattt
    12301 tatatgaata aagctatata caaagattaa gttaggatgt gtaaaaatga ataatacagt
    12361 tcttgttgtg gatgatgaaa aggaaataag agaccttata gagatatatc ttttaaatag
    12421 tggatataat gtaattaaag ctgaagatgg aaaagaggct ctagatattt taaaaaagga
    12481 aaatattcat ttaatgatat tggatataat gatgcctaaa atggatggga tagaagtttg
    12541 tagaaaagtt agagaaagtt tgaatatacc aatacttatg ttaagtgcaa aatcagaaga
    12601 catggataaa atacaaggga taatgactgg tgcagatgat tatttgacaa aaccatttaa
    12661 cccacttgaa cttactgtga gagtgaagtc gctgattaga agagcatatt attttagtgg
    12721 tgctaataat gaagataaag atgtcataaa ggttggagta gtaattatag ataaggaaaa
    12781 acatagtgta acagtggctg gaaatgaaat aattttgaca tcaagagaat ttgatatatt
    12841 atatttacta gcaaataata aaggcagagt atatagtaca gaagaaatat ttgaaaaggt
    12901 atggaaagag aaatattacc aatctaataa tactgtaatg gttcatatga gcagaataag
    12961 ggataaaatt gaaaaatata cagatggaga aaaaataatt catactgtat ggggagtagg
    13021 atataaaatt gaaaaataaa agtttattaa atcatatgtt tcgacccata ttagatctta
    13081 taataagctg tgctttaact tcaatgctta ttttaacaag tatgtatata ataaaatcat
    13141 tatatgtcaa tagtgatatg tctgtagtta atcaacttgt atatcaagca agagatttca
    13201 gaagtcttat agggtatgat ttattttatc catttttatt tacaatcaca tcattagcaa
    13261 tttattccat gatatcgtac aagagaaata agcaaattgt agattttata agaaagacta
    13321 atagaaaaaa tcaaaaatta gaaatcatta aaagtgctgt aaatttaatg gatgaaggta
    13381 acttagaaaa aagtatagat attgagtatg atttagatat attaaaggat aaagaaaagg
    13441 atgatattga tgaactagca cataaaatca ataatatagt ccatcaactt agaaatataa
    13501 ctattgaaga gagacaagct cagcagacaa aaacagattt aataacaaat gtgtcacatg
    13561 acttaagaac acctttgaca tccataatgg gttatttagg agtgatagaa gaaggtaagt
    13621 ataaagatga agttcagatg atgtattatg ttgatattgc atatgaaaaa tcgaaaaatt
    13681 taaatgttct tataaatgat ttgtttgaat tgactaaaat gcaaaataat acaataaaat
    13741 taaacaaaat agaaataaat ttagtagagt tattaagtca agtagtatct caatttgaaa
    13801 tttattttaa acaagaattt atggttagta gaattaattt taatgaagaa aaattaattg
    13861 taaaagctga tccaaataaa ttggtaagag catttgaaaa tcttataact aatgctgtga
    13921 aatatggaaa agatgggcat tatgtagata tagtcacaga gaaaaaggaa gatatggcgg
    13981 ttgtaaaagt gataaattat ggggaaccaa ttccagtgtt agatttacca aatatatttg
    14041 atagatttta tagagttgaa aaatcgagaa atagaaatgt tggaggttct ggattgggtc
    14101 ttgcgattac aaaaaatatt gtaaatttac atgatggaga aattactgtt tcaagcgata
    14161 aatatcaaac tgtgtttgaa gtacaactgc caataataca atcagcttag ttataaaata
    14221 aaagtacgat tatgtgatat aaatattata acaataaagt gtttacaaaa ataaaaaagt
    14281 aatatataat ttaagtcagt ttaataagat acttatcaag agttagggga gagagttagc
    14341 tcagtgatcc tataccaacc ttttatcagg tggtcctgct aacaacgatg agatgacttg
    14401 aaataatata ctcttcttat caataataga taggaagagt tttttttaga attaatgtaa
    14461 caaattttaa agtataatgg aggattaaat aatggaaaaa gtagaaagtt ttaagctaga
    14521 ccatacaaaa gtaaaagctc cttttgtaag aaagtgtagt gtacttgatg gagtaaaggg
    14581 agataaagta actaaatttg acttgagatt tttacagcca aatgtagaaa gttttggaac
    14641 tgctgctatg cacggtttag agcatctttt agctacttat ttaagagata cattagatgg
    14701 tgtaatagac ttatctccaa tgggatgtag aacaggattt tatttaatac tatggggaga
    14761 tgtagatgcc aaaacagtta agattggttt agaagaagca ttaaaaaaag ttcttgaatc
    14821 agataaaatg ccagcagcta cagctataga atgtggaaac tacagagact tatctttatt
    14881 tggggcaaag gaatatgcaa aagatgtttt agataaagga ttttctttaa atatatatgg
    14941 agaataattt aattggaaga gaagatataa gcataaatca aagtgaagtt ttaagatatt
    15001 tacaatatag aggtcaagag atagataaag accttaaata tactattgat gaatgtgtag
    15061 ctataactaa aaataaaata aatcctagat atttatctag gatttatcct attaaaataa
    15121 ataaagataa tgttgtagaa ttagagggaa caaatttaac attagagagt aaagatgtat
    15181 atgaactttt aaaagaatgt aatgagtgtg taattatggc agctactata gggatagata
    15241 tagaaaaaga aataagacga cactcttatt caaatttaac aaaaggacta ataattgatt
    15301 catgtggaac aacagcaata gaagaagtgt gtgacattgt tcaaaataat ttagaaaagg
    15361 tttttttaaa gcaagataaa tttataacta tgcgatatag cccaggatat ggagatttac
    15421 caatagaaaa aaatatagat atattaaacg ttttgaatgc tcaaaaacaa ataggtctaa
    15481 caattacttc taatggaatt atgataccga gaaagtcagt agtggctatt ataggtattt
    15541 ctaatacaag aataaataaa tctaagaaag aaaagtcgtg tgaaaattgt aaaaactata
    15601 gaaactgtac atatagaaaa ggagcgaact cttgtggaaa taagacaata tctgaaaaat
    15661 aatatattaa tatttgatgg tgcaatggga acaatgcttc aacaaaaagg gttaaagtta
    15721 ggtgaaaatc cagaagtatt tggtcttcaa aatccagata aacttataga aatacataca
    15781 gcatacttag aggcaggttc gaatgtaatt cttacaaata cttttggatg taatgaacta
    15841 aaattagata gtaagtatac tgtagaagaa gtgattgaca atgctgtttt ggtggctaga
    15901 aaagctatag aaaatgtaga taatactaaa ccaagatatg tagctttaga tattggtcct
    15961 ataggagaaa tgctagagcc tatgggtact ttaagttttg ataaagcata tgaaatattt
    16021 aaaagacaag tactacaagg cgttaaatct ggagttgatg taatagtaat agagactatg
    16081 atggatttat atgaagctaa agtagctgta ttagcagcaa aagaaaactc tgatttacct
    16141 attttctgta ctatgacatt tgatgaaggt ggaagaagtt ttactggatg tatgccagaa
    16201 tgtatggtgg ctactataga aggtctaggt gtagatgcaa taggagttaa ttgttcatta
    16261 ggacctaaac aacttttacc aatagttgaa aaaatagcat caagagctac agtccctgtt
    16321 atggttcaag caaatgcagg tcttccaaat atagttgatg gagaggctat atatgatgtt
    16381 gatgcaaaag aattttttga aggggttaaa aagttcgttg aagttggtgc tactataata
    16441 ggtggatgtt gtggtacgaa tccaagtttt ataaaagaaa tatctgagaa tataaattct
    16501 gttacaaagg gatgtatcga gaaaattgat aaatgtgtag tctgttcacc atcaaaattt
    16561 gtggaagtac aatcgcctac agttgttgga gaaagattga atcccacagg tagaaaatct
    16621 ctacaagaag cattaaaaaa tgaaaatgtt gattatgcta taaatttagg tttagaacaa
    16681 gtaaatgctg gagcacaaat acttggagta aatgttggtc ttccagagat tgatgaaaaa
    16741 aaactaatgc caaaattaat aagagagatt caagcagtag tagatacacc tttacaagtt
    16801 gattcttcca atgtagaggc cttagagcaa ggacttagat attataatgg tagaacaata
    16861 gtaaactctg taaatggcaa agaggagtct ttagaaagta tattaccaat agttaaaaag
    16921 tatggttcat gtgtggttgg acttacactt gatgaaaagg ggatacctaa aaaggctgaa
    16981 gaaagatttg aaatagcaaa aaggatagtt aatagagcag tttcttatgg gataaaacca
    17041 aaagatatat ttatagactg tttatcatta actgtttcag cacaacaaga agaagcaata
    17101 gaaactataa aagctataac aatggtaaag acactaggag taaaaactat attaggtgtt
    17161 tcaaatattt catttggtct tccaaatagg aaagcactta atgcttcttt tctaacatta
    17221 gctttagggg cagggcttga tttagcaatt ataaatccaa atgaatatag tatgatggaa
    17281 gctatcaatt cttttaaaat attaaataac acagataaag gatgtataaa ctatataaat
    17341 caatatagca atataaataa ttcaaaaaaa tcggattcat ctacaaagat tgataaagat
    17401 ttgcccttgg atattttagt tgaaagagga ttaaaagatg aagcaaaaaa tgtaacactg
    17461 aatcttttaa aagagaagga tgaaaattat atattagatg agattttaat tcctgctctt
    17521 gataaagtag gaaaaagata tgatagtgga gatatatttt taccacaatt gattcaatca
    17581 gcagaaacgg taaaagtttc attaaataca ataaaggaaa ctttattaag taaaagtagc
    17641 aataatgtat ccaaaggaaa aataatagtt gctacagtaa aaggtgatat ccatgatatt
    17701 gggaaaaata tagtaaaaat aatgctagaa aattatggat atgaagttat agatttaggt
    17761 aaagatgtcc ctattgaaga agtagtggaa gttgctaaaa agagaaatat aaaattagta
    17821 ggattaagtg cacttatgac tacgactgtt caaagtatga aagatacaat tcaggcactg
    17881 agagataatg agataaatgc aaaggtattt gtaggaggtg cagttttgac agaagaatac
    17941 gctgaagaaa tgggtgctga ttattattca aaggatgcaa agtctgctgt tgaaatagca
    18001 aagttaaatt tttagcatat aaattaaaat aaaggtttta ctaaatagga tattttatat
    18061 ataatataaa tataagtttt agatacttat gtttaaaaca ataggaatta atctataata
    18121 atgagattaa ttaatataaa aaatatttat catcaacatt aaaaagggct gggaagtctg
    18181 gtataacttc catcagccct ttttagttaa aatcatatgt taaaaagccc aattaccatt
    18241 tttaaaaatc tgtatttgct ttccatcata agtctcacca ataatatcca tatcagcaga
    18301 accaatcata aaatctacat gtgttaaact gtcatttaca ccatgttcat ctaattcttc
    18361 atcttttaaa ttgtttccac cttccagaca agtcttataa gcagaaccaa ttgcaaagtg
    18421 gcaagaagca ttttcatcat atagagtatt ataaaaaatt atatccgtat tggatattgg
    18481 tgagttgtat ggaacaagag caacttctcc taaataatga gagccttcat ccgtttcaat
    18541 aagtttttct agtgtatcta gtcccttttc agcagagaaa tctattattt taccatcttt
    18601 aaatgttaag aagaaattgt caattatgtt tccaccataa acaagaggtt ttgtactata
    18661 aacagttcca tttactttaa acttatgtgg cattccaaaa atctcttcag ttggaatatt
    18721 cggattaaaa cttattccat tagggtcttt ggatgctcca cttagccaaa catgaccctt
    18781 tggcaagtct agagtcaaat cagtttttga ggatttatat tttaaagttt taaagttatt
    18841 tttatttaga aagtccattt tagatttaag attttcatta tgttcttccc atgcttttat
    18901 agggtcctga ccatctactc ttgaacattt aaatatagca tcccacagtt tttctattgc
    18961 tttatcttct gataaatttg gaaacacttt ttttgcccaa tcttttgttg gcacagagac
    19021 tatactccat ttacatttat cagttagagt gtaagaacgc cattctttta aagcttttcc
    19081 agatgccttc tgaaaatttg caactctttc agggtcaaca tcttttaata aatctggatt
    19141 ttgggcatag actgataaaa atgccccacc ttcttttgct atatctacat attgttctgc
    19201 tgtccatttg gggaactcat gaaatgcttc atctggagca tataagtatt taataagagt
    19261 actttcttca tcagaccact ctatataaac atttttagca ccacatttat atgcttcttt
    19321 aagtgcattt cttgcaaaat cagcacattc tattggtgac tttataagta gaatttgacc
    19381 tggctgtatg ttaactccaa cttttattgc taattcagcg tatttttcta aatttctatt
    19441 taaattcata tttatcctcc tatattttta atattgaata acttataaag tattataaca
    19501 ttacttatgt ttttaatatt gaacaactta taaaatatta taactttact tagattttgg
    19561 gtcatattca aaatatttat actttataga ttgagaaata aaaaaaatat tttaattaaa
    19621 acaaattatg aggataacaa acttatttaa gtagtatgtt ttttgttgat aataatatta
    19681 aagtagaaat aaaaatactt ttttagtgaa aagctataca agaataccga aaaaaactta
    19741 taatataata atgagataga tatttataaa tgaaaaaaaa tttttctggc aaagcttaat
    19801 taaaaaggta atagctagtg ttgttattta aattagcata gaaaatcaaa taaaaaaatt
    19861 atgctaatgt atagacattg ataaggtaga atttaagcct tttgaatgct atattaaact
    19921 agagtagata ttcttatgaa tttcaaaata gggcaggagg aaaaaggaaa taaaactata
    19981 aaaaatataa agtatttata taaatttata gagtttatac gtcagaagat ttcagttagg
    20041 agggcataaa tggagcttta tgaagccatt ttttatagaa aatctgtaag aaatttttcc
    20101 agtaaaaaga taaaaaagcc attgatggaa gaaataaaaa gaagttgtaa aaatttagaa
    20161 tacttaaatg aagatttaaa tattaaagca catgttgttg atagaggtca tataatacat
    20221 tttttgatgg ggaaggaatg taaagtaaag gcacctcatt atatagtagt tacatcagac
    20281 aaaggtgagg actatttaca gaatatagga tttgctacag aaggtgtagt tttgcgcctt
    20341 acaacattgg gaatagctac ttgctggttg gaaggaaact taaagagaga agatatcttg
    20401 gaatttgtag atattgggaa taagaatttt gaagaagatg aggaagatga tttagattat
    20461 ctagctggaa taaaaacaga agaagatgat gaaaacaaaa tagaaaatcc ttatataata
    20521 atagcatttg gatatccagc aaaaaatgaa gagttgttta ggacgagata tgctagacct
    20581 gatagaaagc cagtaaaaga tatgataaat aagataaata ggaatagaaa gtggaaagat
    20641 atcttagaat tgacaagaag agctccatct gtaaaaaatt ctcaaccgtg gtatttccat
    20701 aaggatgaac gaggtcttca tttatttgaa aagagaccta agaaacactg tgaagatatg
    20761 aataaggtta gcttaggtgt agccttaaga cattttgata tagcttgtat aaaaaataaa
    20821 atagatgttt catatgaaaa acttcctata aggaataaaa taggaaaatc atattttata
    20881 actgtagtcg agcatgtgaa accagaagaa gaaacccaag aagaaaatgt aactctagaa
    20941 aaagaagaat cgcaagatga ataaaatttt ggaaggggat attggatatg tataagtgtg
    21001 gaaaatgtaa tcttagaggc tgttctaatg gaaatatgga taaattaatg aagatatgtc
    21061 caactagaga aattgaacta caacaaaaag caaagcaact ttatgaagaa gaagagaata
    21121 gaaaaatagc ttataatgct ggattagttg aagcagaggg gtattgtcaa tatactagac
    21181 taaaagagat tatagagttt gctaaaaagt gtggttataa aaagattggc atagcatttt
    21241 gtattggact aaaaagagaa atgcaaacag tgcaaaaaat atttgaacat catgggtttg
    21301 aagtattttc tgttatatgt aaaaatgggg caatgcctaa gagtacaatt ggactagaag
    21361 catctcaaac aatatcaaag tgttcagatg aggtaatgtg caatccaata ggacaagctt
    21421 tatttttaaa tgaagctaag gttgacctta atgtaattct tggattatgt gttggtcacg
    21481 atactttagc catgaaatat atggaagctc ctataacaac tttagttgtc aaagatagag
    21541 taactgggca taatccagtt gctgcaatat ataatgcaga atcatatttt gagaaaaaat
    21601 tgtttgataa agatgaagaa taattcaaag acttattgac ataaaaagca attagtggtt
    21661 taattataaa aaagataaaa ctttaaatta tcctatagat acataaagtt atataaataa
    21721 agaaaaatat tacagctaaa ttccttaaag tagtccagag agactaaagg gttatagata
    21781 atgttagtat ataacctcta gtaaatcttc tagaggtttt tgatgagtat aaaataaaaa
    21841 tttaaatata ataaagtctt ttaaactagt ccagagagac tgggaaagat atctcatagc
    21901 taatgaatag agttatgtat agataaaatc cttatgcctc ttttggtgtg aggatttttt
    21961 tatgattatt taggctttat taaaaaggag atgtaaaaat gataaatggt aaagaaaaat
    22021 tgatagttgc aattgatact gacgaatttg ataaagctaa agaactaata gacagattag
    22081 aagatagtgt agacatattt aaggtagggc tagagcaata cgttgccaca aaaggtaaaa
    22141 caatagatta tcttaaagaa aaagggaaaa aaatattttt agaccttaaa ttccatgata
    22201 taccaaatac tatgaaaagt gcagtgaaag ctgcagtgag agataatgtt tggttaatga
    22261 caatacatgt atcagatatg gaaggaatga gacaatgtgc actaattgct agggaagaag
    22321 cagaaagatt aaatatagaa aagccattag tagttggagt aacagtttta acttcattaa
    22381 gcaaccaaga tttacaggac ataggatgta atatgacaac agaagagtta gctataaaga
    22441 gagctaaact ggcaaaagaa tcaggaatag atggggttgt ttgttcagcg caagaagtag
    22501 ataaaatagt agaagcatgt ggaactgatt ttataacagt ttgcccagga ataagaccta
    22561 aatcagcaga ggttggagac caaaaaagag ttgtaactcc atcagatgca ataaaaaaag
    22621 gtgctcatta tttagttgtt ggaagaccta taacaaaagc agaaaatcca agagaatcag
    22681 ctattaatat tgtaagagaa atagaaaatg cttagataaa atatttggga ggtagcttac
    22741 atgaagggga aattaatttt agaagatgga acagtatttg aaggaaaagc atttggatat
    22801 ctaaaggaag ctattggaga agttgtattt aatacttcaa tggctgggta tggagaacta
    22861 ttgacagatg caacttatta tggacaaata gttactatga cttatccact tataggaaac
    22921 tatggaataa atttagaaga tgttgaatca aataaggtgc aagtaaaagg acttattgta
    22981 agagaaaaaa gtgataaccc aaataatttt agatgtgaaa tggatataga cacatatcta
    23041 aagcaaaata aggtaatagg acttgaagga atagatacaa gagcattaac taaaattatt
    23101 agaaacaatg gaactaagag aggaattata actttagaaa atgtatcatt acaagatgtt
    23161 cagtctaaat tagaagagca ttgtaacaaa gattcagtaa aaaatgttac aagaaaagaa
    23221 attgaaaata taaaaggtac tggagctaaa gttgtagtta tagactttgg tgttaaaaat
    23281 agtgtactag agtctcttaa ggcttgtaac tgtgatataa ctatagtacc agcaatgact
    23341 acagtagaag aaattttaaa tataaatcct gacttaatct tattatctaa tggcccagga
    23401 aatccagagg atataaatga tatagttgaa aatataaaaa aattaatagg caaaaagcca
    23461 ttgacaggaa taggtttagg acatcaaatt ttagcattgg cattaggagg aaagacttca
    23521 aagcttgtat ttggacatag aggaggtaat cacccagtta aatgtttaga aactggaaag
    23581 gtatttataa cttctcaaaa tcatggatat aatgtttcag aaatgccaga aaatacagaa
    23641 gtaactcaca taaatttaaa tgacaatact atagaaggaa tgagacataa ggaattacca
    23701 atatatagtg ttcaatacat tccagaatac acaacaaaag aagatttaga ttgtatattt
    23761 aataaattct tagcattaat atagatttag tattatttac agatagtaaa aaccatttag
    23821 ataaggagaa aaatttatgc ctaaattaga tagtataaag aaaactttag tattagggtc
    23881 aggaccaata ataataggtc aagctgcaga gtttgactat tcaggaacac aagcttgcca
    23941 gtctttgaaa gaggaaggaa ttgaagttgt attaataaat agtaatccag ccactataat
    24001 gactgataaa gaagtggcag ataaaatata tatagaacca ttaacaatag aatttataga
    24061 aaaaataata gaaaaagaaa gaccagacag tttacttgca ggtatgggtg gacaaacagg
    24121 gctaaaccta gcagttgaac tttatgaaaa aggcatacta gataaatatg gagtaaagat
    24181 aataggtact tcagtagaat ctatcaaaaa aggtgaagat agagatatat ttagagaagt
    24241 aatgaaagaa ataaatcagc ctgtaatagt tagtgatata gttacagatt tacaagcagg
    24301 tcttgattat gcacttacaa ttggttatcc agtagttgta agaccagcat acacactagg
    24361 tggaactggt gggggtatag ctgataatga ggaagaatta agagaaatac tttcacatgg
    24421 tctacaatta agtcctgttg gacaagtatt aattgaaaag agtataaaag gttggaaaga
    24481 aatagaatat gaggtaataa gagattcaaa gggaaattgt atagttgtat gtaacatgga
    24541 aaatatagac ccagtcggag ttcatacagg agacagtata gttgttgctc caactcaaac
    24601 tttaagtaac aaagaatgtg caatgcttaa aaaagcatct ttagatatat taaatgcagt
    24661 agaagttcaa ggtggttgta acgttcaatt tgcacttaat ccacatagtt ttgaatatgc
    24721 agttatagag ataaatccaa gagttagtag gtcatcagct ttagcatcaa aagctacagg
    24781 atatccaata gctaaagttt cgtcaaaaat agctttaggt tatactttag atgagataga
    24841 aaatgcagta actaaaaaaa cttatgcttg ttttgaacca actatagatt atgtggtggc
    24901 aaaaatacca aaatggcctt ttgataaatt taaaaaagca aatagaaaat taggaactaa
    24961 gatgatggca actggtgaaa ttatgagtat aggaagtaac tttgaggctg ctattcttaa
    25021 agggataaga tcacttgaaa caggaaaata ttcattagtt cacacaccat ctgaggagag
    25081 aagcattgag gaattaaaga aaagagttgt agtacctgat gatgagagac tatttgattt
    25141 agctgaaatg ataagaagag gctataaagt agaaatgata gaacaaatta caggtataga
    25201 taaatggttt ataaataaat ttaaatggat agtagagcaa gaagaaaaat taaaagttat
    25261 gaaaattgaa gatttaacta aagactatct tcttgaatta aagaaaaagg gattctctga
    25321 taaaggtata tctgacctaa tgaagataag tccagaaaaa ttatatgaat tgagaagtct
    25381 atacaatata aaaccagcat ataaaatggt tgatacttgt gcaggtgaga tagatgctat
    25441 atctccttac tattattcaa cttatgagca atatgatgag gtagttgtta gtgataagaa
    25501 aaaggtgata gtattaggtt ctggtccaat aagaataggt caaggtatag aatttgacta
    25561 ttgttcagtt cactgtgtaa aatctttaag aaaaatggac atagaaacta taatcataaa
    25621 caataaccct gaaactgtaa gtacagactt tgatacatca gataagctgt attttgaacc
    25681 attaacagaa gaagaagttt taagtataat agaaaaagaa aagccagaag gtgtaatact
    25741 acagtttggt ggacaaacag ctataaaatt ggctaaattt ttacatgaaa aaaatatacc
    25801 tatattggga actgattttg atgatataga tgcagcagaa gatagagaaa aatttgatga
    25861 tttactagag agactagata taaatagacc aaaaggaaag ggtgtatggt cacttaacga
    25921 aggtattgaa gttgctaatg agttaggtta tccagtatta gttagaccat cttatgtact
    25981 tggtggtcaa ggtatggaaa taacttataa cgaagaaaaa ttatctcaat acctacaaga
    26041 tgcatttgat agagaccata aaaatccagt acttatagat aaatatctaa ctggtagaga
    26101 aatagaagta gatgctatat gtgataagga agatatattg atacctggta taatgga
//
LOCUS       DAYASV010000045        25804 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYASV010000045 DAYASV010000000
VERSION     DAYASV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.045"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            379..2613
                     /locus_tag="ACGI68_001829"
     CDS             379..2613
                     /locus_tag="ACGI68_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0138270.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIRLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2644..3489
                     /gene="dccA"
                     /locus_tag="ACGI68_001830"
     CDS             2644..3489
                     /gene="dccA"
                     /locus_tag="ACGI68_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP diguanylate cyclase DccA"
                     /protein_id="HFH0138271.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKNKKKF
                     DKNDY"
     gene            3729..5462
                     /locus_tag="ACGI68_001831"
     CDS             3729..5462
                     /locus_tag="ACGI68_001831"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0138272.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDINEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENAQMIL
                     EVNQRCTFEIIEDRGKLLIKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5794..6274
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5860..5882
                     /rpt_unit_seq="ataccacttagttaatataaaac"
     gene            complement(6804..6908)
                     /locus_tag="ACGI68_001832"
     CDS             complement(6804..6908)
                     /locus_tag="ACGI68_001832"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0138273.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7533..7778
                     /locus_tag="ACGI68_001833"
     CDS             7533..7778
                     /locus_tag="ACGI68_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138274.1"
                     /translation="MNQKKIKISIKMVIIGIFSVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIIFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8275..8409)
                     /locus_tag="ACGI68_001834"
     CDS             complement(8275..8409)
                     /locus_tag="ACGI68_001834"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138275.1"
                     /translation="MLDKRLGKRRLKNIYEDIEKENNLVKKFYNIRVESENMILKKNA
                     "
     gene            complement(8531..8677)
                     /locus_tag="ACGI68_001835"
     CDS             complement(8531..8677)
                     /locus_tag="ACGI68_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138276.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8773..9096
                     /locus_tag="ACGI68_001836"
     CDS             8773..9096
                     /locus_tag="ACGI68_001836"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFH0138277.1"
                     /translation="MKDRECSFSPNTIRNRKNMIKNQIEVFFKDMKLIDITPSILQSY
                     INNIYNEHMLNSIKNQVDFIKSVLKESYRLKEISENICDFVTTPIKKVVQLLSFIQNK
                     KLNYY"
     gene            9963..11318
                     /locus_tag="ACGI68_001837"
     CDS             9963..11318
                     /locus_tag="ACGI68_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0138278.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11436..12095)
                     /locus_tag="ACGI68_001838"
     CDS             complement(11436..12095)
                     /locus_tag="ACGI68_001838"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HFH0138279.1"
                     /translation="MLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTISEEH
                     GKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAYGDI
                     DIESHRKWDTDRCEAVGMKVDLPLWQESREELVYEFIDSGFCSVVTKVNLKHLGEEFL
                     GKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGYLEI
                     K"
     gene            complement(12171..12599)
                     /locus_tag="ACGI68_001839"
     CDS             complement(12171..12599)
                     /locus_tag="ACGI68_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0138280.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12751..13299
                     /locus_tag="ACGI68_001840"
     CDS             12751..13299
                     /locus_tag="ACGI68_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HFH0138281.1"
                     /translation="MYTNFNIDFNNFNKKENAIKFMLMGVLLIILGLLCLTFKTLGIK
                     LISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVLIIACILLFVFPQKIQ
                     SIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGICLILSPLFLSRFIVSI
                     MSFFIILFGLVLFFSGNTARKY"
     gene            13418..14353
                     /locus_tag="ACGI68_001841"
     CDS             13418..14353
                     /locus_tag="ACGI68_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFH0138282.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNKIDDTLKARNSYQGCKFV"
     gene            complement(14624..15568)
                     /locus_tag="ACGI68_001842"
     CDS             complement(14624..15568)
                     /locus_tag="ACGI68_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0138283.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKINKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKLCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
     gene            15886..17316
                     /locus_tag="ACGI68_001843"
     CDS             15886..17316
                     /locus_tag="ACGI68_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138284.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGHLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            17516..18100
                     /locus_tag="ACGI68_001844"
     CDS             17516..18100
                     /locus_tag="ACGI68_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HFH0138285.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(18204..18983)
                     /locus_tag="ACGI68_001845"
     CDS             complement(18204..18983)
                     /locus_tag="ACGI68_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0138286.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(19041..20297)
                     /locus_tag="ACGI68_001846"
     CDS             complement(19041..20297)
                     /locus_tag="ACGI68_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFH0138287.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGIAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            20555..21454
                     /locus_tag="ACGI68_001847"
     CDS             20555..21454
                     /locus_tag="ACGI68_001847"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFH0138288.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            21478..22926
                     /gene="ddlR"
                     /locus_tag="ACGI68_001848"
     CDS             21478..22926
                     /gene="ddlR"
                     /locus_tag="ACGI68_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HFH0138289.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            23009..23791
                     /locus_tag="ACGI68_001849"
     CDS             23009..23791
                     /locus_tag="ACGI68_001849"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0138290.1"
                     /translation="MSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSNPNKKGNIK
                     SQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGKSMPTDEPI
                     SFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEKIKLIDDYD
                     AVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDVTPKVSTVY
                     ADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            23817..25736
                     /locus_tag="ACGI68_001850"
     CDS             23817..25736
                     /locus_tag="ACGI68_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0138291.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGFIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKRESVKKDDSTTPKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
BASE COUNT         9879 a         3413 c         3401 g         9111 t
ORIGIN      
        1 atgtataaaa agttaattat gaataaaata attatgcctt tgaaaaatca ttaaacttaa
       61 aattaattat aaaaaaactc taccttacta actatacagc tcacaatcta tctatttatg
      121 aaaaaatatg atataattgc attcaaaaag gaaatctttc aatatactag aatttaagca
      181 ttaactaatt ttatctaata ttattactaa actaaaaaat atttgtattt gtagcattgg
      241 agcaaataac ttaatgtata ttcattttaa atacttattt aaaatttagc atttaaatta
      301 tttacaaatt aacatttaaa tacttctagc ataaatacag ttgtcatcaa aatttaaagt
      361 atcttatgga ggaataccat gcaaaatcat aattatataa aaatgttaaa taaaaaggta
      421 gaagtattat ctaaggaatt agatagtaga aattttaaat taaaaacctt aaatgaaata
      481 gatttttttt ctaaaatacc aaacagagaa tttatgtacc aacatattga tgactgtatt
      541 tcgaaaaata tacatatagg aatcatgtta ttaaatatcg ataactttaa attatttaat
      601 gatatatatg gatacctagt cggagaaaat ttattaatta ctctaactca aaacctattg
      661 cactttattc cagaatcaaa gggaatagtc gcttatttaa aaggagaagg ttttttaatt
      721 gtacttccaa atgtatcaaa taaagagtta aataatatgg gtgcaactat tatagagagc
      781 gcccgagaat taaaaataag acttcctaat gaaaaagaaa tatactccaa tgttacattt
      841 agtatcggca gcactatctg gaatggtgaa ccaaacatga ctactttgat gcttttcaac
      901 caagtgtata gtgctttaaa aaaggctaaa agtgaaggta aaaatagata cattctattt
      961 gattttaaag ataatagttt ttacaataaa ttagaaaatg ataaggagtt cttatcagaa
     1021 atatcacatg ttctacaaac taaacaaatt gaagtatttt accaaccact ttataatata
     1081 aaaaataata ctatagttgg atgtgaggca cttgcaagat ggagacaccc tattaaagga
     1141 cttcttactc cagataaatt tattccttta tttgaacagt ttggtctgat aacggaatta
     1201 gatttgcatt tatttgaaga gaactgtaaa aatataagaa gatggattga taacaatgat
     1261 gtttttgttc caatttgttg taacttttct cgtcttcatt tttttaaccc taattttcca
     1321 agtatgctaa aaggtattac tgaaaagtac agcgtctcta catcaaattt tagcattgaa
     1381 ataacagaaa atatattaat agaaaataca aatacaatta ttcaacaact agctgaactt
     1441 cgttcttatg gtttttctgt atcaatggac gattttggtt ctggatattc atcttttggt
     1501 atgttgcaaa atctaccttt tgatataatc aaaattgata ggatattttt atctcataat
     1561 ttaaatgatt ttaaaaatac aaccattttg tactctataa taaacattgc aagagctctt
     1621 gggatgctta cagtatgtga aggtgttgaa acagaacaac atgtagaatt tattaaaagt
     1681 ataaattgtg atatcgccca aggttattat tatgcaaaac ccatggagtg caaagaattt
     1741 gaaaaattac tatctaaaga gaataattca gagaaaatgt ttataaaata ttctgatata
     1801 ggaaaatctt taatagagaa gatatttgat ggatttttta ttatgcaaga tatgaaaaaa
     1861 ctaagagcta atgtcactga agacattcaa tggtttgatt tttacaacaa aactgaaatt
     1921 acatcttttc ctaaagtaat agagcatttt gaaaaacata ttaaaggtaa aaaattttct
     1981 attctttaca aaagtataac cccacataat actgaatcag attcattatt aattagtgga
     2041 gaagccgttt tatcagatga agcttctgaa catcaaaaat atagcaattt ctactttagt
     2101 gcaaattgta taatttcaga aaataaatta ctcttagcaa aattacattt ggatttaatt
     2161 aaaactgctg gctatataaa tgaatttgaa tcatattcaa tacctaaaat gacaaataca
     2221 catcttgatg aaagtaatat tttatcacaa ttttattcaa tgttgcctgt aggtataatt
     2281 cgctacgatt tatttggtga tatgattatt acttatatga atgaagaaat gtttgatatt
     2341 ataggttata caaaagaaca gttttttggt gagatgggcg gaaatttaag agcaattgtt
     2401 catccagatg atttagactt tgtttataaa aaaagtttgg acatgattga cacaaataaa
     2461 attgaaccat ttttatatag attcataaaa cgagatggtc aaattgtcac agttatgtat
     2521 acacaatgta acctttctgc aattgatgga aggtcgattg cccagggtat gtatgtagat
     2581 gtaaataaaa taaaaaattt aacaaaaatc tagtacaaag tattttattt tggagaaatt
     2641 aatatgttta aagaaatttt tttaagaact tttccaggat ttctaattat aagagattct
     2701 aactacagaa ttatatttat aaatgataat ttaaaaaatt taattaaaag ttatatgcaa
     2761 gataatccac ttggaatgac taatatagaa atagctaaga aactccctga taatattgct
     2821 aaatttttta cggattctca caatattcaa ttagattggg aaaaaaatta tccttatgat
     2881 aaaatttcaa attggatttt agagtttaaa aaagatacta caagctattg gaatgtttta
     2941 gaatacaaag tagatgttga tgaaaaaacc tacataatta ctatggccaa tgatataaca
     3001 aaactctatg aagaaaataa gagaaatctt cactactcaa ttactgaccc attaactgga
     3061 gcatataata gaaaatattt aaatgataga tttgacatat ttataggaga ttatattgta
     3121 ctaattgact tagataattt caaaatgata aatgactatg aaggtcataa tgtaggagac
     3181 aagattttat gtgattttgt ttcactttta aataaagaat taataaattc tacttctatt
     3241 atacgattag gaggagatga atttattgtt atattctcta gtgatgttga caaaaattat
     3301 gtttattcac agctagaagc cttaagagaa aattttttaa aagtattctc taagtataag
     3361 tatctttcat ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt
     3421 gaattggata aaaaaatgta taaaaacaag gagaaaaaca agaaaaaatt tgataaaaat
     3481 gattattaac attgttattt taaaataaat taaaattatc tttctttttt taaacttgta
     3541 cattagttta taaatatgta tctatacaag atattgacat taagtactga agcatatatt
     3601 gtttcagttc ttttttgtta aaattaactt atttaacaaa gatatcatag tttaacaaag
     3661 cttttaattt aaatatattg atgtataata taattaaaat ataatagcaa aaagaaagga
     3721 ggaatatcat gagcatactc ctaaaaaaag cacccaaatt agcaaaacat attataacaa
     3781 gtttttatat aaatcgagat atcaatgaag tattaaaata cctttgtgaa aatgttacat
     3841 ggattggacc aggagaacag gaatttctca cttcttttaa tgaaataaaa aattattttt
     3901 atgcaggtca agatgaaatt ccttcttgtg atataaataa tgatatattt gaaactgtaa
     3961 gtgaagatga gaatagatgt atggtattag gaagatatac tgttcgaact aaagaaaatg
     4021 ctcaaatgat tttagaggta aatcaacgtt gtacatttga gataattgag gatagaggaa
     4081 aattattaat aaagcacatg cacatatcaa acccttatgg tgaaatgcaa cttgatgaat
     4141 actttcccac taaaattgga actcaaagtt atgattattt gcaaagatta ctaaaagaaa
     4201 aaacagaagt tattgaaatg attacaaata atatcaatgg tggcttaaaa ggtagcaatg
     4261 atgatagtac atactcattt ttctatgtca atgagggatt gcccaaaata ttaggataca
     4321 catatgatga gttcatggaa atgagtggtg gttcagcagt gggtgctgta tatccaccag
     4381 acttacctaa agcactagaa gattgtgaac gatgttttgc aaaaggacct acatattcat
     4441 ctgaatatag aatccgaaaa aaagatggaa cattaatgtg ggtattagat tctggaatga
     4501 aatcgttaaa tagtgatggc atagtaaaaa ttaatagtat tattactgac ataacccaac
     4561 taaaaaacat agaatctgaa ctaaaattag aaagagagcg atatcgtatt gctttgcaaa
     4621 atattacaga tatcatgttt gagtatgata ttgaaaacga tacccttata aaatatcaac
     4681 gtgtagaaat tgacaaaaaa attgaattag aaaattttga aacaaaaaat tactccaaac
     4741 tattagaatc tggtaaaatt attcaccttg atgatattgg aaaactacta gaagtacttc
     4801 atggaaatct tcgtgaaaca attgagataa gacaagtaaa ttcattaact aaaaatgaat
     4861 ggagatggat tcgtgttcaa tgttcagtta tatatgatag tgaccataac cctatcaaaa
     4921 caataggcgt tttaaaagac ataactgaag ataaatctaa attagaaagt ttaattaatc
     4981 aggctcaacg tgacccacta acacaattat ataatcaaag agtatcacaa aatcttattg
     5041 aagagtatct atgtagttct gattccaaaa ataatgatgc tctattagtt attgatattg
     5101 atgattttaa aactgtaaat gatacttttg ggcatctaga gggaaatgaa gttttagttg
     5161 ctgtatcaaa aatactatta cataacacac atgataaaga catagttgct cgaattggtg
     5221 gagatgaatt tacaatattt ataaaatcat taacaaagga tttaataata aaaattacta
     5281 atgatatttt aaatgatgcg agtaaaataa aagtaaagga caatcataaa ataacattaa
     5341 gtattggtat tgccttcact gatgattcaa ctaaattata taaagattta ttttctaaag
     5401 ctgataaagc tctttatcta tcaaaagcag atggcaaaaa ttgttatagt acatacgaat
     5461 aataaagcta tttatacgac caagaatgaa cctttaaatt taatttcata gtctaacaat
     5521 caaagagttg aagctaatct tatagctagt caactagttc ctagaaaaaa tttaaacaat
     5581 atatttattg atactgctca tttttctaaa gagcaaattt atataaaaaa ttaaactttt
     5641 atatacatac agatactaat gataaacagt tatcattagt atcttctatc ttaaaacaaa
     5701 atgataattt atctatataa aaaagtgctt tgttgacaga gttcactttt ttatataggt
     5761 acagaattta agtataaata catgttttat tctatacatt ttagttaatg taaaatttct
     5821 tgattgtact ctctaacttt tcaacattat actatttata taccacttag ttaatataaa
     5881 actataaaag tgttgcaatt actgggtttt ataaagttaa tttacatacc acttagttaa
     5941 tataaaactt tagcacctta tttatctata gagctacaga aagtatttac ataccactta
     6001 gttaatataa aacatgtatt tatttgttct tgtactattg ttttactcta tttacatacc
     6061 acttagttaa tataaaacaa tatcaaaaca aataaaaatt tttaataaat cattatttac
     6121 ataccactta gctaatataa aacattctgg tactgttgct atttatgttc cgtattcaga
     6181 atttacatac cacttagtta atataaaact ttggagttgt tacagattat ttgccttatc
     6241 tctctattta cataccactt agttaatata aaacccccaa ataaactggg cattttcaat
     6301 acttacacat atgcaactct cttaaatttg cagtgaacca tgagtagtgc aaatgataaa
     6361 gcttatcata taccctcaac accttatatt tcaagcgtta agctctactt tgtaacaaat
     6421 atcgctcact gcaaaactat ttacattttt attatatcat aaatatatta tttttgaata
     6481 tctataccaa tttgtggtat aataaaagca agaagaacta caatctattt agacgtagag
     6541 tgaagttcaa atactaatgt ttattctttt tgaacttgat ttttagttca aaactaaagt
     6601 cactcctgcc tgtgtgaact atacccgctg ttttatacag ttaaaacatg taaaaatagt
     6661 tataaagtac tagtgtaact cattagtact ctttttatta tattcatata attaatatct
     6721 atattttaaa gcttactatc taaaaaagag cacgtcaaat aaaaccctag atatttaatt
     6781 tttttagaga tacttaaaat aaattatgct ctctctgtac ttaaatattt cctaaattca
     6841 aatggagaca tagaatttaa tctattttga tatctaaaat tattatagta gtatatataa
     6901 tcatctattg ctttaactaa ttattcatat gtatcaaatt tccttaaata gtacatttct
     6961 acctttaaaa ttccccaaaa tccttccata ggagcattgt caaggcaacg accaactctt
     7021 gtataatata tgtatatcta acaccattaa gaaatatata ttttataata tatatttctt
     7081 catttaatgc acttagaaaa gcaaagaaaa tacagattag tacacttttt agagaaaaat
     7141 aaaattaata catctaaaat attaaaaaat actttagata atctaaagac acatctcaaa
     7201 gtttttaact aaattataaa actaattaca aatgctatgt aacgataata aataaaaaat
     7261 ttacttcgct taagcgacgt gtcgttgaaa tatttcatgt tgccaactga ttcataagtc
     7321 gataagaaaa gaacactaat tatttagagt tcttttctta gctacaagtg gattataata
     7381 cttttaaatc tgtgagaaaa tcatatataa taatgatatg aacaagtaaa gaaaatacaa
     7441 aaatgacaaa attataaata aaatattata aaatcataag gtcataattg ttatattgta
     7501 gaaacaaagt aaagagagag ggtggagaat aaatgaatca aaaaaaaata aagattagta
     7561 ttaaaatggt aattataggt atattttctg taattattgc ttttgtatta tccagattat
     7621 tcgttgagta tattgatgaa ttattaaggt ctaattggca atatcagcta tttgaaagtg
     7681 agagttcata tataatattt gccaattgta ttatgttctc gaatatatta ttagaaatat
     7741 ttttaatata tatttgtaga aaatttagaa aaatctaata tggtcaacgt atttcgaatc
     7801 gaatacttca tgattattta gtctattgtg ctagttaaag tttaattccc atataaagta
     7861 aattgatttt agtaaaacaa atcaccccta agggttagtt tatggataag tcatccaaat
     7921 attaactctt taatttttgc tatagattca tctttgctca taactttact tataaaaaat
     7981 gaaatattat aagctagaac ttttattcta attctagtca taaatcctag catagagtta
     8041 ctttttttat attataaata aaactataca acaaagataa tatttagtaa aaataacttt
     8101 cattttaact aaacttaaaa ttcaattact ctctaaattg acgtttagga attagttcaa
     8161 aataaaaaag agtgtatata ttgaaattac tacatttcaa tatatatact ctttttaaat
     8221 ttctcagttt tccatggaac tttactttat ttatttctgt attagttgta ttttttatgc
     8281 atttttcttt aaaatcatat tttctgattc aactcttata ttataaaatt tctttactaa
     8341 attattctct ttttctatat cttcatatat attttttaat cgccttttac ctaatctttt
     8401 atctaacatt actaatgatt taactaatat attatatgat tttatcgcat cttctattga
     8461 gatatttaaa catatattta ctgcttcata aaaatcaaat tcattaaaaa atccattttt
     8521 atttacaatc ttatatccct ttgcatctct ttctaaatac tcttcctaaa tactcttcaa
     8581 aactttcatc atctaatcta aatcctatat cactatgaaa aataggtttt gcatttggat
     8641 atagttctat tgttttatca aaagtttcga ataccaagtt attattattc gattttccaa
     8701 tagtccacta taaacaataa taagtttgtt gctccaaata atgtaacact tgtagaaaga
     8761 tgttacagat atataaaaga tagagaatgt agtttttctc ctaatactat tagaaataga
     8821 aaaaatatga ttaaaaatca aattgaagtt ttctttaaag atatgaaatt aatagatata
     8881 acaccttcta ttttacagtc ttatataaat aacatataca atgagcacat gttaaattca
     8941 ataaaaaacc aagtcgattt tataaaatca gtattgaaag aatcttatag attaaaagaa
     9001 atatcagaaa atatttgtga ttttgttaca accccaatca aaaaagtagt tcaacttcta
     9061 agctttatac aaaacaaaaa gctcaattat tattagaaaa atcaaaaaat attcctatag
     9121 gtataccaat ttttttgatg ctaactcttg gattacgttt tggtgaagct gtatcactta
     9181 tatggtcaga tgtagacctt gataaaaaat ctcacatgta aatcaaacat tagtttatgt
     9241 atacaataaa ataatcttca aagaccctaa aacccctata tcaaaaagaa aattatttgc
     9301 tcctgatgaa cttataagtt tgcttaaagg ggaaaaatta aaacaaaata agttaaaatt
     9361 acagggtata ttgaaaaatg aatttgattt gatatgctta aataaaagat tcaacccgtg
     9421 agttcaacca acttttgttg ttcattttaa aaaactatta agagataata atcctagaga
     9481 tataaaagta catgaattaa tacatgcaaa tgcaactttg atattacttt caggtactaa
     9541 cataaaagct atttataaaa tattagtaca tgcagatata aaaatagcta tgaatatata
     9601 ttccatattc tcacttatta gatgaaatga ataaagaaac atctgaaaat ataagcaaaa
     9661 tactattcaa ataatgttca gttttatgtc aattaaatgt cactcaaagt gctttttttt
     9721 catttgtcag ttaaatgtca cttaaagtac ctttttccta tttgtcagtt aaatgtcagt
     9781 aatatataaa taagatatat ttataagacc cttaaaatca ctattattta ataaaattta
     9841 taattatata ctatttgctg ttgacattgg tattatacca tagtttatca tttatattag
     9901 aaataaaatt attaattaat agtttcgtaa ttttataata gatttacagg gagtgatagt
     9961 aaatggatac aaaaatatct ataaacgaaa acatgagtct aggtaaaaga ttttttaaat
    10021 acctagcacc atctgtagtt gcaatgtggg ttttctcact atatacaatg gttgatggaa
    10081 tatttgtaag taaaggagtt ggagaattag ctttagctgc tgtcaatata tcaatgcctt
    10141 ttattaattt tatatttgca gtttctctat tattttcaac tggtgcatct accataattg
    10201 caatctatct ggggaaaaaa gatattaaat ctgctaacga agtcttttca tttaacttag
    10261 tatcaataat tatattatca ataattatat tagctataac tttttttaat ttagatagat
    10321 tagcactatt tttaggagct acagaatcta caattggtat ggttaaagat tatttaggaa
    10381 taataatatt ttttaacgga ttctttatag tgtcttattc tttagaagtt ataataaaaa
    10441 ctgatggctt tccaatatta gctactgttg gtgtaataat atctgctctt acaaacatca
    10501 tacttgatta cttgtttgta attgaatttg gatggggtgt aaaaggtgct ggtattgcaa
    10561 ctggtttgtc tcaagtcttt tctactattt tcttcttaat acatttttta agaaaaaact
    10621 ctactttgaa tttttcaaaa tttagaattg attttaagac acttagaaaa attgtattta
    10681 taggttttcc tgattcaact actgaattaa gttgtggtat agttgtttta ctttttaact
    10741 taagtcttac taaatatatt ggagaaaatg ctcttattta ttatagtgtc ataaattata
    10801 taaatacatt agtacttatg actatgatgg gtataactca aggtatgcaa ccacttacta
    10861 gcttttatta tggtgcagga aatatagata atgtaaaaaa attattaaaa atggggataa
    10921 aagccacaat aatagcatca gttgctgtat ttgcaatttg tatggctttt tcaggaccaa
    10981 tagtctcttt atttatacat ccagaagaaa caatgctatt caatgaagga gttcgtgtat
    11041 ttaaaatatt ctctatatct ttcttattgg taggaattaa tgtaataatt tctggattct
    11101 ttgtatcagt agaaaaacca tcaatttcta ctgttatatc tcttggtaga gggcttgtaa
    11161 tagttgtttt aagtctaatt tcaatgatac taatatttgg tggacaagga atatggatga
    11221 ctactattgt ttctgagttt atatgtctta ttttatcatt agtttttcta aaaaagaatt
    11281 tttctacatt agactctaat ctaaataagg ttgcttagat gataagagtg acaatatatg
    11341 aaaatatcta aataaaaaat acctattata caatcttgca aatattaaac ctttgcaaaa
    11401 attaggatta tataataggt attttattta tgtaattatt ttatttctaa atatccatag
    11461 ccatctttta ttaaagttcc ttttacttca tattctacag gttttttaaa tattggacca
    11521 tctacgacaa atgtatgata ctctccatgt tctccacatg ggtctgcacc agcatttttt
    11581 atcttctcca caagttctct agttaatttt tttcctaaaa attcctctcc taagtgcttt
    11641 aagtttactt ttgttacaac actacaaaat ccactgtcta taaattcata tactaactcc
    11701 tctctactct cttgccataa tggtaaatct actttcatac caactgcttc acatctatca
    11761 gtatcccatt ttctatgact ttctatatct atatctccat atgcacatat agttgctccc
    11821 atgtttttag cttttattaa agccctctca aagctctctt catatccttc tacaccacac
    11881 tcagcaacta ataaaggtaa tcctatattg ctacttactt gcttaagcat attatattct
    11941 aaattatgtg tccatgactt tccatgttct tcacttatag ttgtcaagag tgccacaggc
    12001 ttatatcctt ttttcaacat tcgatttaat gctaatatac tatccttacc accactaaat
    12061 gacattacaa atttttcatc agccaatttt aacacctctt tcatctaact ttgttttatt
    12121 aacataatta taccaaattt ttgagttgca tagcaaatta tattaaattg ttaattcact
    12181 ataaagaatg tccctaatat taaaagtata acccctatgt atttgtataa atgcattgtt
    12241 tcttgtccca aaattacgct atcaataaaa aatccaacta aaacttggga tacaagagtt
    12301 aatataagtg tatttgatac tcctaaattt ggaattgcct ttactgtaaa atatattatt
    12361 agacctccaa atactcctcc taataacaac ttataattaa aaccatttat agatactaga
    12421 cactttaagt cacctgtaac tattatactt gccaaaaaaa atattgctcc tacagttaag
    12481 cttataaagg tggccactaa tgctgttgta ctttttccta gctctccatt tataaaagct
    12541 tcaagagctg tagaaccacc tgctaataat gcaaatatta ttgcccataa ttttgtcatg
    12601 gacaaacctc cttctatata attctattaa tagaagttta tgatacaacc tattaattaa
    12661 ttactaagta tagtttaatt tttatacact aggctttatt tggatatgaa tttctgttat
    12721 aattgttcta tcgattttgg aggtgatttt atgtacacaa attttaatat cgattttaat
    12781 aactttaata aaaaagaaaa tgccattaaa tttatgttaa tgggagtatt actcattata
    12841 ttaggattat tatgccttac atttaagaca ttaggtataa aattaatttc ttggactttt
    12901 ggtattgcac ttttattctt tgcatattta aatttaaaaa atattaatga actaaaaaga
    12961 tatgctacta aagaagaaat taaaccctct attaatatcc aatgggttct aataattgca
    13021 tgtatattac tttttgtatt tcctcaaaaa atacaaagca tattttcatt atttttagga
    13081 ttttatttaa tctttaatca actagtagca cttgtaaata gtaagaataa tccttattct
    13141 aaatttacaa cttggaatat agttaagatt ctatttggta tatgccttat attatctcca
    13201 ctttttttat ctagatttat agtatcgata atgtcttttt tcataatatt atttggacta
    13261 gttctatttt tctctggaaa tacagcgaga aaatattaat tattttctcg ctgtattttt
    13321 atattactta aaatagaatt tgtttaagtt atctatttgt ggtaatatgt tttttgtaca
    13381 agctcagcgt acaattttat tagaaatagg tgattttttg gataaattta aatatgcttt
    13441 tgataataaa agatatcata cttggaatta ctatcttaga aatacttttg gagaaaaagt
    13501 ttttaaggta tctataaatg caggatttac ttgcccaaac attgatggaa gtcttggtta
    13561 tggcggatgt acctattgta gcaaagaagg ctcaggtgat tttgctggaa accctaaaga
    13621 taatcttatc agtcaatttt ataatataaa agaaatgatg ttaaaaaaat ggcctcatgc
    13681 aaagtatatt ggctactttc aagcctatac aaatacgtat gctcctcttg aagttttaaa
    13741 agaaaaatat gagactatat tagaacttga agatgtcata ggtctttcaa tatccacaag
    13801 acctgactgt ttgccagatg atgttgtaga atacttgtct gaactaaata aaagaaccaa
    13861 tttatgggtt gaacttggac ttcaaacaat tcatgattct acatctaaaa ttataaatag
    13921 aggtcatgat tataaaacat ttgttgaagg tgttgaaaaa ttaaagtcta aaaatataaa
    13981 ggttgtcgta cacattataa atggtcttcc tggtgaagat tataatatga tgatggaaac
    14041 agcaaaggca gttggtaaaa tgggtgtaga cggtataaaa atccatctat tgcatgttat
    14101 aaaagatact cctatggaaa agatgttaca aaatggtatg ttaactctta tggaacaaga
    14161 tgaatatatc aatcttgtct gtgaccaatt agaaatactt cctgaaacta tgataatcca
    14221 tagactcact ggtgatggta agagggatga actagtaggt cctatatgga gcctaaaaaa
    14281 atgggaaatt ttaaataaaa ttgatgatac tttaaaggct agaaattctt atcagggctg
    14341 taagtttgtt taatacaaaa taataactat agaaatggtt atacaaatat agtcctttct
    14401 atagttattt agtatttaca aaacaaaata ttaatttgta catttataca atatataata
    14461 aaaaatagat agtctaaact gtatctactt atattttatt tgtactgtcc actttattct
    14521 ataaaattta taaatagtta ttttttaata ccaaatataa tttggtataa tttttgttgg
    14581 aatctacaat gtataccaat aaatcctttt taagtatata attctaaata ctttcagttt
    14641 tttcaatctg ctttaacttg ttggacttat aaaatattct tatttcatca taagaaagat
    14701 taagtgctac tactgcagat attaacatca acatcactcc aaataacttc cctaaggaaa
    14761 aactttctcc taatatagtg caaccaatta ttactgaacc aactaattct actgatgcca
    14821 caacacctgc aactgacaac tctacacctt tagatatacc tgctgcataa aaaatatatg
    14881 caagtgcagc tggaacaatg cctaacatta gcatacaaga taaaacatct aaatccttag
    14941 cataatttaa gatttcccaa ggtcttgaag aaggaatcat aaatatagct ccaaataaga
    15001 aactgtatac taatattgtt gaacttgaaa attcctttaa agcattttta ctaatagttg
    15061 gcataagtgc atatgctatt gctgataata ctccaagtaa aagcccaaaa gcgtttaaac
    15121 cttgaaaatc caaacgtcca ccagtcactg ccataattgc accaataaaa caaagaataa
    15181 gtgaaaataa tttgcttcta gtaatatttt ctttataaca taattttgaa aatattgcta
    15241 aaaacagtgg tgatgtatat agtaaaactg ctgcaattga tacacccgca atgtcaattg
    15301 ctttaaaata acataaatta aacattgcct gacatataat tcctattatt actgaatata
    15361 taattccttt tttgtttatc tttaagattt gtggtgtcct tatagatgaa tatacgccta
    15421 aaactataaa tccaaaaaac aatcttgtaa atgctacttg ttctgatgtc agtccatttc
    15481 ccattaaaaa ctttccaaat aatccaagtg tagcccataa taaccctgct attgctataa
    15541 atacataccc tttacttttt tctcccattt taaattccct tctattttta tttagtcaat
    15601 ttacactctt ctttttaagc aaaaactatg ccatcttaaa tcaatatata tcgactctaa
    15661 aaccctagtc tttccaataa tactaataaa ttttcaattt ttttatattt atttttcgct
    15721 tactttaagt tgatttagac ttattttaat tgtaaaatga gttatatata acttatttaa
    15781 agttaaaata gactgaaact tatatataaa tatcaaattt taagtatatt aatcaattaa
    15841 acctatttta atatatcatt aaaaatttag gaggaaaata taaaaatgaa tttagatttg
    15901 gaattttatc aaaaaatatt agaagcttcc catgatgaaa tatgtgtttc tgatgataaa
    15961 ggaataataa tatattgtaa taaagcattt gaagaaaatt atggtctaaa aaaagaagat
    16021 attttgggaa aaaatgtttc ttttttagaa gactctggat attcaacaaa aagccccata
    16081 ccaattgtat taaaaacaaa gactaaattt tcactggaac aagatactca aacagggaaa
    16141 aagcttatca taactgctac ccctatattt gatgaaaatg gacacttaga atttactgtt
    16201 gaaaattgta gagatataac tgaacttaat aatataaaaa ataaacttga agacacaaaa
    16261 aagcaagtaa aaaaatataa atctgaagta gaaacactat acagaactgc acttagaatt
    16321 gaagatactg taattatgga cggaatagtt atgagaccaa tcataaacac agtaaatcat
    16381 gtttctaaaa cggatgtaag tgtactttta ttgggtgaat ctggaacagg aaaaagctca
    16441 cttgctagat atatacacca caacagcaat agatctaatg gcccatttat aacaataaat
    16501 tgtgcaacta tatctccaca acttcttgaa tctgaacttt ttggatatac ttcaggtgct
    16561 tttacaggtg caagtactaa aggtaaagtt gggctcgtag aattagctaa tggaggaact
    16621 ctttttttag atgaaatagg agatatacca caaaatctac aggcaaaatt tttgcaatta
    16681 atacaggata gaacttttac tccagttggg agcttaaaaa ataaaaaggt cgatataaga
    16741 attatatctg ctacaaatgt tgatttagtt tcaaaagtaa aagaaaaaaa gtttagagaa
    16801 gatttatact atagattaaa tgttatagaa ataaaattgc ctcctcttag agaaagaagg
    16861 gataatctag tggaaattat aaaatattat tttaataggt actcaagtga cttcaaccta
    16921 aataaaacta tttctaagga agctatggat gctattgcaa attatagatt cccaggcaat
    16981 ataagagaat tacaaaatat aattcaaaaa atcttgctta cttgtacaga taatcatata
    17041 acaatagatg acttaccaaa cattcttaca aaaaatatac atattaccaa taatggaaat
    17101 aaaacacata tatcccaaat taataaggtt attatatctg actcaaaatc tactaattat
    17161 aaaaataaaa attttgacac tttaattaaa gagtatgaaa aaaatataat tttggatgct
    17221 tatgaaaaat ttggtagttc ttataaagta gccaaacacc ttgaaatctc acagtctaaa
    17281 gccaataggt taattagaaa gtatactaat acttaatttt aaatttgata aataaaaact
    17341 taacactatt tacatattgt aaatttggcc atttactgtt attatgaata tggttgaaat
    17401 tatttaaata aatactgttg acttagtttc atatatttac cattaaactt ttatcataaa
    17461 tatcatatca taataatata tttaataagt ctaataaact atacgaggtg taaatatgtt
    17521 aatgaatacc cattttatta tggcaaaatc aatacttgat aatatagatg aaaataaaac
    17581 attctttata agcgaaaaaa attttatata tggcaatata aagcctgatg ctttttctaa
    17641 atataaatta aaaaaacatt atttagatga gtcatttaat atgattgttt ctaaaataaa
    17701 ctatctatgc agtcttaata tagatagtct ttctaaatac ttttcagtaa gtagacttag
    17761 tcaggaacta ggagtcatat gtcatttttt atgtgatttt ttctgtgtag ctcacagtga
    17821 acgttgggaa tttaagcata gcttaaataa acatgttact tatgaaagag aacttggagc
    17881 atttgcaaaa gatgttgatt tatctaaaat taaatgtggt actataaata gtagttttga
    17941 gaagtttttt actaaacttt atagtgatta caaaaagaaa actgattata tgaatgattt
    18001 aaaattttca gcttatactt gtaatagtgt cataaattat atacttgact ctattttatc
    18061 taatacaata aaatcatatc atatagcaaa ttgcggttaa cttaagaatt tttaaactta
    18121 tataaactta taattgaata taattaaaat gactgctcta tagaatataa ttttatactt
    18181 ctctaggaca gtcatttttt atactaacta tttatctcca ttataacatc tatggcactt
    18241 tttacactat ctactacata atttgcactt tgctttacat cttcccttgc cttagacatt
    18301 gcaaaactat atttaaatcc ttctaacatg caaatatcat ttggagaatc cccaatagta
    18361 gcaacttcat ctggacttac atttaacgtt tttattacat gctctatact tcctcttttt
    18421 gacacacttt taggaactat atttaaagta tatgttccag aaaaatatat ttctaagact
    18481 tcactaaact cactttttag ataaagttct attctggata actcttccaa attttcagaa
    18541 catacgccta tatttcctat aagattatca aaactacttc ttttttcaaa atctgggtca
    18601 actataaagt cactagaata atgtctaaaa gcagtggtat cactacccac aattacatca
    18661 cccttatatt taaaataaag ttgtgcatca cttgactcaa atctatcaat gacttttttt
    18721 actaaattat catctatcgt tcttttaaaa attacattat aatctttatc atatataaat
    18781 gaaccatttt cacatacata gtatccattc atatctaaac tatctttgat aatattatgt
    18841 gcttgtttaa aaattctacc tgtagcaaca ttaaactgta ctcccttttt ttctatttct
    18901 tcaattgcta ctatatcttc ttttgttatg gttccatttt catatagagt tccatctaaa
    18961 tcacaaaata tatgttttat cataaattaa aagcctccaa aaacaatttt atattttact
    19021 aatactttaa tcaaaataac ttaaagcttt tctaattgta ttctcaattc attaagcaaa
    19081 tctctagtag ctcttaatct tatctttctt ctatccccat taaatatatt tttctttact
    19141 attgtttttc catttatata tattgcagta taaaccagtc ccacaggctt ctctttggtt
    19201 cctccaccag gtccagctac tccagttgta gatataccta tattagcttt aagtccttta
    19261 gcaatgccct cagccatttc tatagctgtt tgctcactta ctgctccaaa ctcttccaaa
    19321 gtttctcttt ttactcctaa tcttttcatt ttagaatcat tagaatatgt tacacaacct
    19381 tcaagaaata cagaggatat acctggatag tttataagag atgaagaaac caaacctcca
    19441 gtacaagatt cagccactgc tattgttaga tttttttcta caagtagctt agaaacagca
    19501 tcttctaaag cagtctcccc ttcagatatg gaaatatctc cttcagtata tacatattta
    19561 ccaactctac tttttatttt ttcttctact ggttttatta atttaaaagc ttctctttca
    19621 ttttcagcct ttgcagttat tcttattgta acttctaatt cttttgcata taaagcaact
    19681 gttggattag tctgctcatt aattatatcg agaatttcta attctagatt tgactcacct
    19741 attccatata atcttaaagt cttagatact agcatttcat ttgtgaattg ttgtaagtaa
    19801 ggtttgacac tctcattaaa catagctttc atttctcttg gaggtccagg caatactatt
    19861 ataaatttac catctttttt taaaattgcc cctggagctg taccattatt attttttagt
    19921 actatagcat cttttggaaa gtacacttgt ttcatattat tttcggccat ttttttccct
    19981 gttttaacaa agaaagattc caaaacctct agtgaaggtt tatgtagttc taatttctga
    20041 ccaaagtatt ctgcagcagt ttcttttgtc atatcatcac cagttggccc aagaccacct
    20101 gtagtaatta caaaatcact tctctttaag ctttcatcaa aacattctaa aagtctttgt
    20161 ttgttatctc caacagtact ctgatggtat acttctattc ccaatgatgc aagttcttta
    20221 gctaaaaatt gagaatttgt atttactata tctcccaata atatttctgt tcctacactt
    20281 attatttcag ctttcaaaat ttatcactcc ttgatttata gccattaaga ctagtcttaa
    20341 gttcataaca ataattttag caaaaataat aacataaata aagttaaaaa atcatttaaa
    20401 catatcttta aactagatta ttattctaaa accacacata atataaactt cgtatatagt
    20461 ggtactttca tcctcaactg ttaattcata taattaagta tgtaaataaa aagtttactt
    20521 taaaataata aaaattaaaa taggagtgta taatatgaaa atagcagtta taatgggtgg
    20581 aatatcatca gagagagaag tttctttaaa ttcaggaaaa gagatatata ataaccttga
    20641 taaaaataaa tatgaagttg taaaggtaat aatagatgat aaaaaagata tatttacaaa
    20701 gataccagaa gatatagatt ttgcaatact tgctttacat ggaaaatttg gtgaagatgg
    20761 atgtatacaa tcaatacttg agactatgga tataccatac tctggatgtg gacctctttg
    20821 cagtgggatg tgtatggata aaaatataac taaaaaaatg cttagagatt caaacctacc
    20881 tactgctcca tgggttttag taaaatcagt tgatgaaata gactatgatg aaatagataa
    20941 tataggttat ccagtattta taaaaccaaa ctcaggaggt tcaagtgtag ccacattctt
    21001 tattcattct aaggatgaag ttgaagaagc agttagaaaa ggtctggaag ttgatgaatt
    21061 tgtaatgatt gaaaaatata taccaggtgg agaatatact tcattcatat taaatggaga
    21121 agtgttccct acaatatcta taaaatctga ttcaggattc tttgattatg aagctaaata
    21181 ctcagttgaa aaaggtgcaa aggaagaagt tgtttactta gatgaagaac ttcaaaaaag
    21241 agtaaacgaa atttctgaga cttgttggaa aatttttaat tgcaaagcat atgtaagagt
    21301 tgatatgatt ataagtgaag gtattccata tgttcttgaa ctaaatactc ttcctggaat
    21361 gactcaaact agtttaattc ctagaagtgc tgctgctaga ggtattaagt acagtgaact
    21421 attagataaa cttatagaat actctttaaa ttagatgaaa taatttgagg tggtaatttg
    21481 atattttcaa atctaaaatt aaatgataat gaaccaattt atatacaact aaaaaattat
    21541 ataagtgata tgatatcaaa aggactaatt ccagacaata gtaagctccc ttctacaagg
    21601 gagcttagtc agcttttaca agttagccga aattctgtag ttttaactta tgaagaatta
    21661 aaatcagaag gattaatcta ctctatttca ggaaaaggta catttgttaa atcaaagaat
    21721 aagtcttcaa atactacatg gtcattaaac tgggattgtt tagagaatac atactctaaa
    21781 aaagccaatg aattggacat tataaaaagt gaaattcctt ggagctctga cctgatttcc
    21841 tttaaaagta tttctccaga tggagattta ttcgacatgg aagaattaaa aaaatccttt
    21901 ttaaatagaa tttcactaga aggacataaa ttactcaact atggatatgc acaaggttat
    21961 aagccactta ttgactattt actagaatat atgacaaaca aaggtgctga tataagtaat
    22021 aaagatatac taataacaaa tggattcaca gaaggctttg atattattat gtcatctttt
    22081 acccaagaaa aagactacat aatatgtgaa aatcctactc ataatactgc tataaaaatt
    22141 atgaaatctc ataacctcaa tatcttaggt gtagatataa atgaagatgg tttggacttc
    22201 aatatgctag aagacaattt agttaagtat aaaaataaaa taaagtttgc atacattaca
    22261 ccctcttatc acaatcctac aggaattgta atgactcctg aaaataggta caaattctat
    22321 aatcttatga aaaaatacaa tatagcaatt atagaagatg gatttaatga agaattactc
    22381 tacaatagtt ctcatatatt tcctatctgt tccctagata atatgaataa tggggtcgta
    22441 tatattggta gtttttcaaa aattttattt cctggaatga gaattggatg gatatttgca
    22501 gataaaaaag taatcaataa gctagaaagt gtaaaaaggt gcagaaacat acatgtatcc
    22561 tttttagacc aaggaatttt atttgattat ttaagtaatg gaggttttga aaaatacatt
    22621 aaaaaaatac gtaaattcta tggagacaaa tttaactttg catataaatg tataaaaaaa
    22681 tatataaaaa cagaatatat cttgggtgat ggtggtttgc atatatttat aaaactaaaa
    22741 aatatagata ctagagtttt attagaaaaa tgttatgaaa aaaatgttat atttatgcct
    22801 ggagacttat tttttacaga taactctggt tttgatactt taaggctcgg attttctagg
    22861 ttgtcttttg aagacattga aattggaata aaaataattg gagaaactat aaatgaaatg
    22921 ttctaggtta actaaatatt gttagcctag tttattttta tgaggtataa tatatatagt
    22981 ttatatgaat ctaatactgg gggataatat gagtaaaaag tcaaaacgaa gaaaaattaa
    23041 taaattaaaa ttaacgattg taataactgt attatttctt gctttattat ctatatatga
    23101 gatactgttc tctaatccca acaaaaaagg taatataaag agtcaagaaa ctatgcaaaa
    23161 taaaaataat acaacagatg attctaaaaa taatcaaagc tctaccaaaa agcaggaaga
    23221 taataatatc aaacctactt tttcatattc ttcaatacca gataatataa aaaataaaat
    23281 gattggtaaa tctatgccta ctgatgaacc tataagcttt gatagtctat cttatttaaa
    23341 acttacatat tatggatttg atgaaaaaac tcatcaagga gaaatgatag taaacagtga
    23401 actagctcca gaagtagtgg atatttttaa ggaattatac gaaaaaaaat atccaattga
    23461 aaaaattaag ttaatagatg actatgatgc tgtagatgaa aaatctatgt ctgataataa
    23521 tacctcatca ttttgttata gaactatagc tggtacaaat gtagtttcaa atcatggtaa
    23581 aggacgtgct atagatataa acccattaca gaatcctcaa gtttctggaa atgatgttac
    23641 acctaaagta agtacagttt atgctgatag gtctagcaca aaatttggaa tgataaaaaa
    23701 aggtgatgat tgttataatg cttttgtaag cagaggttgg agttggggtg gctattggaa
    23761 aaacccagat tatcaacatt ttgaaaaata atagattaga aataaaggtg aattatatga
    23821 atttaatgac attggaaaat ataagtaaaa gctattctga aaaaaaatta ctagaaaata
    23881 tatctcttgg aataaatgaa ggggaaaaaa taggatttat tggtgtaaat ggtactggta
    23941 aatctacctt actcaaaatt attgcaggag ttgaagaaag tgagactggt aatataacaa
    24001 aaactaatgg tattaggata gaatatctac ctcaaaatcc aacttacaat gaaaattcaa
    24061 ctgtacttga acaagtattt aatggaactt ctgaagaatt aaagctactt ggtgaatatc
    24121 aagaaatcct agaaaaactt aattcaaact ttacagagga attaaataaa aaattattat
    24181 cagtacacga aaaaatagat gccttaaatc tttgggattt agaaagtgaa gctaagtcag
    24241 tacttacaaa gcttggtata aatgacttta atcaaaaaat agcagaactt tctggtgggc
    24301 aaagaaagag agtctctctg gcatcagccc ttattacacc ttgtgagttt ttgatactag
    24361 atgagcctac aaaccatcta gacaatgata ctatagattg gcttgaggaa tatcttaatt
    24421 ccagaaaagg ttcattgctt atgataactc atgatagata ctttttagac cgtgttacaa
    24481 atagaattat tgaactggac aaaggtagat tattcagtta tgatgggaat tattcaatat
    24541 ttttggaaaa gaaaatggaa agactttcgc tagaagcaag tattgaagat aaaagacaaa
    24601 atttaataag aacagagctt gcgtgggtaa gacgaggagc tcaggctcgt agcactaaac
    24661 aaaaagctcg tttacaaaga tttgatgaac ttataaatag agattctttt aaacctgatg
    24721 aaaatctcga tatatctgtt gcatcaagta gacttggaaa taaaataata gaaatacaca
    24781 atatatctaa atcatttgaa gaaaatactg ttatagataa tttagaatac actttaactc
    24841 gtacagatag aattggtata ataggcaaaa atggtatggg taaatctacc cttataaata
    24901 tacttaatgg agaaatagag cctgatagtg gatatatttc aattggagaa acagttaaaa
    24961 taggttgttt ttctcaagac gactctcata tgaatattga tatgagggct atagactatg
    25021 taaaagaagc tagtgactat atagaaactt cagatggtac taggattaca gcttctcaaa
    25081 tgtgtgaaag attccttttt aatggtacta tgcaatatac tttgataggt aaattatctg
    25141 gtggtgaaag aagaagacta catcttctta aaatccttat gacagctcct aatgtgcttc
    25201 ttttagatga acctacaaat gatttagata ttgaaacgct taaaatatta gaagaatacc
    25261 tagacgaatt taaaggtgta gtagttacag tatcacatga tagatatttc ttagatagaa
    25321 tttgtaataa aatatttgca tatgaaggta atggaaaaat tcatatctat acaggtaatt
    25381 acagtgatta tcttatttat agagagattc aaggaattga atttgaagaa tctaaaaggg
    25441 aatcagttaa aaaagatgac tctactaccc caaaaaaaga aaaacctaaa aatgataaaa
    25501 agcttaagtt ctcatataat gaacaaaggg agtttgaaaa tattgactct gatatagaaa
    25561 aactcgaatc taaaattgca acacttgatg aaagtactgc aaaattttca actgatttta
    25621 ctaaactcca agaaatttta gatgagaaat ctaaactaga aaaagaactt gaatacaaat
    25681 atgaaagatg ggaatattta aataatctag ctgatgaaat agctaataat aagtaatatt
    25741 aaaataatct taagtttaat aagttttgct ttaaatctgt aatttataaa tattttaaag
    25801 gaag
//
LOCUS       DAYASV010000046        25761 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYASV010000046 DAYASV010000000
VERSION     DAYASV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.046"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            101..772
                     /locus_tag="ACGI68_001851"
     CDS             101..772
                     /locus_tag="ACGI68_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138292.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            774..1682
                     /locus_tag="ACGI68_001852"
     CDS             774..1682
                     /locus_tag="ACGI68_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0138293.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1759..2445
                     /locus_tag="ACGI68_001853"
     CDS             1759..2445
                     /locus_tag="ACGI68_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138294.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            2442..4997
                     /locus_tag="ACGI68_001854"
     CDS             2442..4997
                     /locus_tag="ACGI68_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0138295.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKKNKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIEVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5191..6390)
                     /locus_tag="ACGI68_001855"
     CDS             complement(5191..6390)
                     /locus_tag="ACGI68_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HFH0138296.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6509..7396
                     /locus_tag="ACGI68_001856"
     CDS             6509..7396
                     /locus_tag="ACGI68_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0138297.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7586..8143
                     /locus_tag="ACGI68_001857"
     CDS             7586..8143
                     /locus_tag="ACGI68_001857"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0138298.1"
                     /translation="MSDSEYPQMKTLETRSCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8382..9242)
                     /locus_tag="ACGI68_001858"
     CDS             complement(8382..9242)
                     /locus_tag="ACGI68_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0138299.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            9792..11144
                     /locus_tag="ACGI68_001859"
     CDS             9792..11144
                     /locus_tag="ACGI68_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0138300.1"
                     /translation="MIFICLILTVIGIFLSLITIQKPNGEFIRSDWPKKFTENFNEQI
                     VFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPKEHKDYYSNSELLALANQ
                     NNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGKFIGGKSIVFSIIGIIFL
                     IILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVFADIYNSLNILNEEIHKS
                     DEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSTNYSPEKVYKYASIMLKN
                     IEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSIDILNYPEYESNIVEFKSN
                     IDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHVTLNERIEIIISDNGKGMSRE
                     EASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNISVESIPGLGSSFYITFPI
                     "
     gene            11363..13840
                     /locus_tag="ACGI68_001860"
     CDS             11363..13840
                     /locus_tag="ACGI68_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0138301.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNTIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSEELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGNMNEEFIEDVRHMEGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGVTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            13855..14541
                     /locus_tag="ACGI68_001861"
     CDS             13855..14541
                     /locus_tag="ACGI68_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138302.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            14815..15648
                     /locus_tag="ACGI68_001862"
     CDS             14815..15648
                     /locus_tag="ACGI68_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138303.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSWMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            15686..16147
                     /locus_tag="ACGI68_001863"
     CDS             15686..16147
                     /locus_tag="ACGI68_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0138304.1"
                     /translation="MKFGGVLLIVKDINKSKKFFEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQV"
     gene            16178..16426
                     /locus_tag="ACGI68_001864"
     CDS             16178..16426
                     /locus_tag="ACGI68_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0138305.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVKDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
     gene            16879..17199
                     /locus_tag="ACGI68_001865"
     CDS             16879..17199
                     /locus_tag="ACGI68_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HFH0138306.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            17337..17981
                     /gene="trmB"
                     /locus_tag="ACGI68_001866"
     CDS             17337..17981
                     /gene="trmB"
                     /locus_tag="ACGI68_001866"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFH0138307.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            18125..18856
                     /locus_tag="ACGI68_001867"
     CDS             18125..18856
                     /locus_tag="ACGI68_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138308.1"
                     /translation="MNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDANLRAVV
                     KRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEVPKEAL
                     SLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHNVDIAK
                     GESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGNKTGKA
                     VVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(18985..19263)
                     /locus_tag="ACGI68_001868"
     CDS             complement(18985..19263)
                     /locus_tag="ACGI68_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138309.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     ILVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            19538..19984
                     /locus_tag="ACGI68_001869"
     CDS             19538..19984
                     /locus_tag="ACGI68_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138310.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            20254..20706
                     /gene="accB"
                     /locus_tag="ACGI68_001870"
     CDS             20254..20706
                     /gene="accB"
                     /locus_tag="ACGI68_001870"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFH0138311.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            20722..22083
                     /gene="accC"
                     /locus_tag="ACGI68_001871"
     CDS             20722..22083
                     /gene="accC"
                     /locus_tag="ACGI68_001871"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFH0138312.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            22098..22952
                     /gene="accD"
                     /locus_tag="ACGI68_001872"
     CDS             22098..22952
                     /gene="accD"
                     /locus_tag="ACGI68_001872"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFH0138313.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            22973..23917
                     /locus_tag="ACGI68_001873"
     CDS             22973..23917
                     /locus_tag="ACGI68_001873"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFH0138314.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            24106..24366
                     /locus_tag="ACGI68_001874"
     CDS             24106..24366
                     /locus_tag="ACGI68_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HFH0138315.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            24519..24890
                     /locus_tag="ACGI68_001875"
     CDS             24519..24890
                     /locus_tag="ACGI68_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0138316.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            24920..>25761
                     /locus_tag="ACGI68_001876"
     CDS             24920..>25761
                     /locus_tag="ACGI68_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138317.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNF"
BASE COUNT         9997 a         2857 c         4036 g         8871 t
ORIGIN      
        1 aaatacaatc tttttagtga aataaataaa attaaaatat ttttagtatg ataatgcaag
       61 aaaataatta tttatctaaa aaatctaaag gagagtaaaa ttgaacttat taattattga
      121 agatgatata aacttaaatg aaggtctttt ttatgcattt gagaatgatg gatttaatgt
      181 atttaaggct tatacaaaac aagaaggatt aaatatattt aatagtaaaa atatagattt
      241 tataatctta gactgcaatc ttccagatgg agatggattt gatgtatgtc aaataataag
      301 ggaaaagtca gatataccaa ttataatgct tactgcaaga gactcagaaa ttgatgaagt
      361 aaaaggatta gaaatagggc ttgatgatta cattacaaaa ccattttctt tatctgtatt
      421 aaaggcaaga gtcaaagtag caataagaaa aaaatcgaat aataaagtaa tatattccaa
      481 tggaataaaa ttggaccaaa agttactaaa agtatataaa aataaagaat gtttggaact
      541 tagctctgta gaatataagc ttctaagtta tctaattgaa aataaaggtc aaatattatt
      601 aaaggaacaa attttacacc atatttggga cagcgaagaa aattatgttg atgataatat
      661 tgtatctgta aatataagaa gacttagagt aaaagtagag gatgacccat ctaatccaaa
      721 atatataaaa acagcttatg gaatgggtta tttatggaat gaggtagaat aatatgttta
      781 tattagcctt tataatatgt acagttgtta tatttatagt atctactaaa ataaatcgaa
      841 aaagatatga tgaattaaat ttgatgttaa atcaaatatt ggaaggtaaa gaagttactt
      901 atccagatac aaaggataca agagcgtcaa aaatttctca ccaagtaaaa aaaattaagg
      961 atatgataga aatagaggta gaacagtcaa agtcagaaaa agaagcgatt aaaggtttaa
     1021 tatcaaatat gtcacatcaa ttaaaaactc cactttcaaa tataaccatt tattgtgaac
     1081 ttctggagaa tataaatatt tccacattac agaagaagga gtttttacaa aagatgaaaa
     1141 atgaaacttt taagattgac tggctgttac aatccttatt taaaatgact aaattagagg
     1201 atggagttat agaatttgaa gtagaagaac tactaattaa agatactctt attcaaagta
     1261 tcagtactat ctttaataaa gcagaagcta aaaatataag gataaatctc gaaccatttt
     1321 cagacataaa attagttcat aataaaaagt ggaccataga agcaattgta aatgttttag
     1381 aaaatgcaat aaaatactca ccatcagata gcacgattac tatttctgta ataaaaatgg
     1441 aactatatac gaagattact attaaagatg agggaatagg tattgatagt agagaactaa
     1501 atgatatttt taaaagattt tacagaagta aaaatgttga aaaccaaaat ggcacaggga
     1561 ttggtctata tcttacaaga ttaattttag aaaaagaaaa tggaaacatt atagtagaat
     1621 ctaaactagg aagtggatgt tgttttagta tcttcttaca gaattgtaag agtttaaact
     1681 aattttgtag gatgagtgta attatcgtca cttacaatat tttatagaac atatatctgt
     1741 attgaaagga gaaattaaat gagtatatta gtgacgaata atttagtaaa acactatgga
     1801 aaaggtgaga ctaaagtaaa agccttaaat ggagtaagta tagaaattga acgagataca
     1861 tttactgcta tagttggaac ttctggttct ggaaaaagca cattgttaaa tattataggt
     1921 ggattagata atccaacctc aggtgatgta ataattaaag gaaagaacat atctaaaata
     1981 ggaaaaaaag atttgacagt gtttagaaga agaaatatag gatttatatt tcaaaactac
     2041 agtttaatgc ccgtattaaa cacttatgat aatatagctc ttcctgttac attagataaa
     2101 ggaaaccata tagaccatga atatattgaa atgctgatga agacattggg aatttgggat
     2161 aagcgtttaa aatttccaag cgaactatct ggtggtcaac aacagagggt agcaattgct
     2221 agagctttgg caaataaacc tgcgcttata ttagcagatg aaccaacagg aaatctcgat
     2281 agtaaaacta caatggaagt ggtatgttta ttaaaagaaa gtagtgcaaa attccatcaa
     2341 acgatactta tggtaacaca caatgaaaat atagcacaag tatgcgactc aattatccat
     2401 attgaagatg gagttgtggt gaatactggt ggtgaagtac tatgatgtct ctagtaaaat
     2461 tttcaattca atatataaag cattataaaa aacaatctat atctatagtt ttaagtatag
     2521 tactatcagt agcattattg acgggaattg gttctttagt tcatagtgca gacaaaagta
     2581 gaattgaaaa gataagggaa gatagtggag attatcatta tttctataaa gtagataagg
     2641 aacaacttaa taaaattaaa aaaaataaaa aatcaaagga atacactgta gatagacttg
     2701 gcgttacaaa acctaaaaat cagatagatg aaccttttat tatagaattt ttaaatgctg
     2761 attcttcata tttagacatg acaggtagaa aattattaaa aggaactctt cctagtaaag
     2821 atggagagat agtacttgac acatattctc taaacaattt aaatgtaaac aaagaaattg
     2881 gaacagaaat caatttagat ggtaaaatct ataagttatg tggtatttta tcaagtcttt
     2941 caaacccaga aactgctctg gagggatttg taaacgatta ttttcagcct aaagaaaagg
     3001 attcttatat agtttatgta aaatatgatg aaaataaaaa tattaaaaag caaaattata
     3061 aatttatgaa aaaattcaac atatcaaaag aaaaaactta tataaactgg cctctttcag
     3121 ttgaatttgg agtaaaacca ccaaaaaata tagaaaaata taatatattt gagttcttaa
     3181 aaaatttaga actaaataca aatgcaatta tattacttgt tggagtgttt ggtgcagcag
     3241 cagtatatag tattttccat gtttcaatac ttcagagaat atcacaatat ggggttttag
     3301 aagtattagg cgctaataat aaacaacttt tatttctact atttttagag ttatttttac
     3361 tttttattgt tggatttcct ataggttgct tcttaggtat tgaagtggcg tctacaatat
     3421 atgaacaatt tccacatata tttttgagta gtgatattgt tccaggagca ttttttataa
     3481 gtgaaaaatc tatttattta ggctttttat ttatcttatt gttgttaatt ttaattgctg
     3541 tacgtgttgt acatcagttg aataaatact ctagtataga atctatgaaa aactttaaga
     3601 ttactaataa gaaaaagaga atattagaaa gtgtaaaaca ctctaacatg acaaaagtat
     3661 tatctcataa atacatgact caaaaaaagg gaatgtttat tggaattttg ttttcattat
     3721 cacttggtgg aattatattt ttatgttcta gttactctat acagcttact aaaaataata
     3781 atgaacttac aatgaaagct gatgatggtt taaattctga ctaccaaata actatgcaaa
     3841 caacagattt tgatgtaggt ataccaaaag ataaaatttc aagattaaac aatgttgagg
     3901 gaatatcaaa agtctatcca gtaagatatt tatttggagc tatgcaaatt aaagaagaac
     3961 aactattttg gaaaaatttc tttaaacctc ttgaaaaaga ttacaggata aagacttttt
     4021 ttgatggtat ttgtacaaag caaaatgaag gtggctatct tttaaaaaca aatatttatg
     4081 gttataacaa tggtatgtta gaaggattaa attcttatat tatagatggg gagattaata
     4141 cccatgatat ggttaaaaat aataaggtta tagttagact acctatggat ggaactggga
     4201 tgtatgatgc agttgatata aatccaggag atacaataaa agttaaagtg ccaaagacaa
     4261 tgaaaccaac agatgaaatt gttaaattta aagaagaaaa taagaatgat tatacaacaa
     4321 aagaatttgt agttgctgct actgtaaaac gtgtgatggc aaataacata tatttcattg
     4381 gtgattatgg tatggatata gttatgacga atgggcaaat gaaaaataac tttgatatcg
     4441 aaaattataa ttcagtaagt ataaaaaaga cagaagatag taacagtgag gaattgtctg
     4501 agaacataaa aagtgttgta agtgatgtaa aaagatgtat cgtaacagat tataccattg
     4561 ctattgaaaa aaataatacc tacttaaatc aaaaactctt atttatatat ggtattgtat
     4621 ttgtacttct tgcaataagt ttgttccata taatcaatac agtaagctac ttaatttttt
     4681 caagaagaca tgagtttgga attttaagag ctatgggtat tacggataat aagtttttac
     4741 ttatgatgat aagagaaggt tttttatatg gattctatgc aagtattata atggtaattg
     4801 gaagtgttat tggacaattt atgatttatt ttatggtaaa acgtgtatat ttatatataa
     4861 atccaattct taaaataaat acacctctat acataggtat gattatttta aatatcacta
     4921 ttagtatagt tgctgtaatt attccagtaa gacaaatttt gaagagtgat attatttcag
     4981 aaataaataa gaattagagt tatataaatt tttaagatta tataactttc tgacaatctt
     5041 gtttaaatta tttaaatata tcttatctgt gaacattaaa taaacctctt aatagatata
     5101 actcttgaaa agtcatatct attaagaggt tttaataaat tttcatgtta atatatataa
     5161 tccaaaaaaa ttaaatctta ttaaaacata ctattttgta tagttatcga aatatccttg
     5221 tatgtaaata ataggagtac ccttatctcc acttccagat gttaaatctg ctaaagaacc
     5281 tataaggtct gttaatcttc ttggagtagt accttgagaa accatgtttc ctgttaaatc
     5341 atcagattta ttatcttttt caactatgta ttttgaaatt gcttctttta actcatcacc
     5401 agataagtca gcaaaatcat tatctgctaa gtattttaat tttacttcgt ttggagttcc
     5461 ctctagccct tttgtataag caggagatac tactgggtca gctaattccc atattttacc
     5521 tacagggtct ttaaaagctc catctccata aaccattact tctacatctt tccctgttat
     5581 ttcttttata ttaccttgga ttttgtttac aacttcatca caatttatag ggaataattt
     5641 tactgtttct tctgtagctt tgtttgaacc taaaagtcca tactggtcat tgtaaccact
     5701 accatctaca cttgctgtca ttatatcatc aagtgaatat acttttttag ctccattttg
     5761 tcttaaaatt ctctttgttc tttgtcttgt atgtatatca caagttaaaa tacttgttgt
     5821 atagttaaga atagtttttg gattgtttgc aaatactatt tctacctctg caccacaatc
     5881 cttgattaaa ttcttataat aatcaacata atctacacca gtaaatgtat gcttgttata
     5941 tccaaatagt tctctgtatt tttcttctgt taaaacatct gaccaaggat ttattccctt
     6001 atcatcaagt tcatcaatac ttattaagtg atttccaact tcatcagaag ggtagcttaa
     6061 cattaatacg acttttctac atccttttgc aattcctttt aaacatatag caaaacgatt
     6121 tctacttaaa attgggaata taactccaac agtatcatct ccaaatttat cttttacatc
     6181 ttttgcaata ttgtcaacgt gagcataatt tccctgtgct ctagctacaa cagcttctgt
     6241 aacggcaatt acatctttgt ccctaacttc aaagttttca ctttttgacg catttaaaac
     6301 agaatcaaca actattttaa ctaagtcatc tccttgtctt ataataggtg ctctaacccc
     6361 tcttgaaaca gttccaacta atctatccat tatctaaaat cctcctaaag aattaatatt
     6421 gtcatattag taccatgtat attataattc aaatataatt ataagtaaaa taaataatac
     6481 taatatttga cataaggagt aattatatat gaatataaaa cttgaacttt ataaggtttt
     6541 taatgcagtt gtaaataata agagtttttc tcttgctgct aaggaattgt ttatgtcaca
     6601 gccagctgta agccagtcaa taaagcaatt agaagaacaa ttagatactt tattatttta
     6661 tagaaacaat aaaggagtaa aattaactcc agaggggaaa attctaagtg aacatgtcac
     6721 tactgcatta aagttaatat catcaggaga agatagaata aataaattta aaaaattaga
     6781 atacggttct ctgaaaatag gagtaggaga tacagctgca cgttttttct tgttaaaata
     6841 tttagaaatt tttcataaga agtatccaca tatacatgta tctacaataa atagaacgag
     6901 tagagaactt atatctttac tcaaagatgg gaatatagat atagcaatta taaacatgcc
     6961 aatagaagat gatacactaa atattgttga gtgtattgaa atacatgata tatttgtatg
     7021 tgcaaatgat tacatagaat acaaaggtag gaaaatatca ttggaagaat taaacacatt
     7081 gcctttaata atgctagaaa ataaagcaaa ttcaagactt tatgtaaatg aatatttttt
     7141 atcaaaaggt ataaaactaa acccagatat agaattgggt tctcatgaat tacttttaga
     7201 atttgcgtat ataaatcttg gagtgtcttg tgttatagaa gaatttagca tagattattt
     7261 ggaaaatgag aaattgttta aattagatat aaaagagcca atacctaaga gaaatatagg
     7321 ttattgccat ttaaaagata tatctttatc cttagcaacc aaagaatttt taagcatgat
     7381 atctaataat atataattta gaatacatat ttcacaagta atttaatata aaaatagaga
     7441 taaattcgta aatttgaaag attcatatag taattagttt ttattaaaaa taaaaataag
     7501 gttaatttga gaacatttat ataattaata tcgtatttta aatatgaaca gtatatttag
     7561 attaatgtat aaagggggat tagaaatgtc tgattctgaa tatcctcaaa tgaaaacctt
     7621 agaaactaga agttgtatac taagacctgc atcattagat gatgttgaag atttttttga
     7681 ctgttataaa aataaaatag tagtaaaaca tttaccattt agtgagcata aatctttaga
     7741 agatacaaga aagtttataa aatcattttt tctaaatcca tataaaaaag ggaaaattgg
     7801 ccactttgct atagtatata aaagagacaa taaagttatt gggaatatgg ggtttaataa
     7861 tataaatcct aaagcattag aagcagaaat aggtatatgt ataaatccaa gctattgggg
     7921 acatgatttt gctactgaga taacaaaaag agtaatacag tatggattta gggaactaaa
     7981 cttaaataaa ataattgcaa taacctatga agaaaaccaa aattctacaa aatcattaga
     8041 tttattagga tttaaatgta caggaaagta tatcaaaaaa attcatactg gaaatactgt
     8101 aaaaaatgtt ccttgttatc aatatgaatt gaaaaaacaa tagatataat catttaatat
     8161 ctatgctggt tatttagtaa tgattgtttt ctaaataaaa tttcaataaa gtggttgcct
     8221 caaaatatag ataatcctgt ttttattgtt aaaatactga aaatgcattt gattttatac
     8281 aaaaaagagt gtctcatgaa ctaaaaagtt tatgagacac tctttttgaa taaatcgtat
     8341 tttaattaaa atagtatata tacttaaaat attaattttt attagttctc taaattttta
     8401 aatccttctc caagaacctc atgtacatct gtaacaataa caaatgcagt agggtcagtt
     8461 cttttaacta atctctttag ataaagttct tgttttttag aaactacgac taatagaact
     8521 tctttatttt ctctagtgta tccaccttta ccatcaagaa tagtaagacc tctatcaagt
     8581 tcttcagtta tagcttgtct taaactttca ggctcatcag agataataaa aaatgctttt
     8641 gaatggtcaa atccttccat tatcatatct gctattttta ttaatacaca aagtgctata
     8701 ccagagtaaa gagcagtttc taaactacga tttactatac cagaagatat aacaaccatt
     8761 ccatctaggc atgacatgaa ttttgtagct tttattcctg ggaatttctt actaagtatg
     8821 agtgcaatta agtctgttcc tccagtagaa gcatctgatt taaacattat tcctaaacct
     8881 ataccaacta ggattcctcc tgcaatagaa gacaataata agtcattagt aaatcttagt
     8941 tttgataatg ggtctgttaa ttgaactata gctgaaaata caactgttcc aaataaagtt
     9001 tttaatgaat tttttgtacc cataattcta aaagcaagta ttactaatgg gacacctata
     9061 attaacatga tagctgatac tggaagtcct gtaaccttgt ttagtactaa tgatagacca
     9121 ctaagtcctc caggagcaat tgtatgtggt tttaaaaaca tatttatacc aatactcatt
     9181 gatatacaac caaaaaatag tccaagcatt tcaagtaaaa ttggcgactt ttgttttttc
     9241 atataaacac ctccatgaac ttgtcacaat ttaatattat aacatatttg accagtttat
     9301 attatataaa atttcttgat ataagcaatt tcaagtattt cattattctt aatatttttg
     9361 aatttttata tagtgttttc atatctttac tgatgttgtt acactcctat gaagtttttt
     9421 atatattata atttttttat tcttttataa agtatcccct ataaagatgg ttctatgaaa
     9481 attttctcta tattgtttaa cgtatataga gattttgtgt ataattataa ggtatttaat
     9541 ggaaaaattt atcttacttt tatatgttat caaaatgatt tattaaatac aacagacagc
     9601 ttgataacat gtaattatat aaatataatt tatgtagata gttgtatttt taaataaatt
     9661 tttacatatc aataattatt gtctatttta tataagcaac aatcaaacta aaataaaagt
     9721 ttaagaaaag agttgattaa atgcataaac ctagttataa aacagtattt cattcataca
     9781 ttattttttt catgattttt atttgcttaa tcctgacagt aataggtatt ttcttatcac
     9841 ttattactat tcagaaacca aatggggaat ttattcgtag tgactggccg aaaaaattta
     9901 cagaaaactt taatgaacaa atcgtattta tagataataa gccacaagta aaacagtctg
     9961 gattagaaaa acttcgagaa aatcaattgt ggttgcaaat tttagattct agtggaaata
    10021 gagtttatgg gtttttagaa ccaaaagagc ataaagatta ttattctaat tctgaattat
    10081 tagcattagc taaccaaaat aattcaagta atggaactgt atactttaat acaattaaaa
    10141 ataataaaaa tgactatatt tatattatat attttccatt aaatatatct aaagtgaata
    10201 tgttttttga tggaggtaaa tttataggtg gtaagtcaat tgtattttca atcataggga
    10261 ttatattttt aataattttg gtttctggaa ttgcttatgg ttactgggta acaaagtgca
    10321 tatcaaatat tacatctgct gttagtgata tttcaaaacg aagctatctt ccagttaata
    10381 ttaaaggtgt ttttgctgat atatataata gtttaaatat tttaaatgaa gaaattcata
    10441 aaagtgatga gatacaaaat aaaactgata ttatgagaga agagtggatt tcaaatataa
    10501 ctcatgattt aaaaactcca ctttcaccaa taaaagggta tgcagagctt cttacagata
    10561 gtagtactaa ttattcacct gaaaaagtat ataaatatgc ttcaatcatg ttaaagaaca
    10621 tagaatatac aaatacactt ataaacgatt taaaattaac ctatcaatta aaaaatggaa
    10681 taatcccttt aaataaaaat aattatgatt tagtaagatt cttgaaagaa ttatccattg
    10741 atattttaaa ttatccagaa tatgaatcaa atatagttga atttaagagt aatatagata
    10801 atattaactt tgattttgat tgtacactat taaaacgagc ttttagtaat cttattgtca
    10861 atgcttttac atatggagat aaaaatacta aagtttgtgt acatgtaaca ttaaatgaaa
    10921 gaattgaaat cattatatct gataatggaa aagggatgtc aagagaagaa gcatcaaatt
    10981 tgtttaatcg ttattataga ggaagcaata caaaacaaaa aatagaagga actggtttag
    11041 gactagcaat aacgaagcaa attattgaag ttcatggagg gaatatatct gttgaaagta
    11101 tacctggatt aggtagctca ttttatatca cctttcctat ttaattaagg ttaattaagg
    11161 ttaaataaaa aatatattaa ggttgataga ttattatatt tatatagtag tttatcaatc
    11221 ttttttattt aaggggtgat ttgattaaaa atattataaa tagaatgata aaagtatgga
    11281 ggagtctatt agagatttaa tttagattaa agtataaaaa tattagattg aaatatctta
    11341 gataagggaa gaaggtgtaa gtttgaaaat tatattaaag tatattttca caaatattaa
    11401 agaacgaaaa attagaacag cagttatgtt attgtccata gttttatcaa cagttttact
    11461 ttttgtttca ttctcaattg gattatctta tgaaagtgca caaagaaaaa tggcaaaagg
    11521 tatgtatggt acagcgacta tatctgtcca aagtaaaaat ccagatatat taaccaattt
    11581 agaagacata cctgatttaa atactataaa atcaaaggtt ggtgtattgg aaagctctgc
    11641 tatttataat aaagggggat actatgaaga atttagtctt atatcagcag acttaagtca
    11701 actaaataaa atcaacaagc cacgattaga aaatggcgat agtattactg atttttctgg
    11761 tgataaaatt attcttccaa atcgctttac atcaaaatat aaaataaaaa aaggtgattc
    11821 tattactttg cagatttatg gaaagtctta tacttttcaa gttagtgata ttgcatctta
    11881 tgatacagta tttttgagga acacaagagg tgtaaatgca ctgctaccca aagagactct
    11941 atcaaaaatt ataaataaag gcagtggata tacaagagtt cttatagagt cagaagaaga
    12001 aattacagaa aatctagtaa ataaattatc cgaagaactt tctactgaaa aatatatggt
    12061 atcaaatact ataaatgaaa ctaagataat ttcagatgct agacaaaaga caatgccttt
    12121 ctttttaata agtttttttg cattaaccct tagtatattt ataatataca gcagttataa
    12181 ggttattaca ttagaacgtc ttccttttat tggaacattt agaagtattg gtgccaatga
    12241 aaaaactgta actcgtatat tgatgcttga aagtatatta tacggaagta taggaggatt
    12301 aattgctatt cctattggtg tagttgtttt aaatttaatg cttcatgggt taggaaattc
    12361 tctcgaacaa ggaatatcaa ttccagtagt aatatcaccc attggagtga ttatatctgt
    12421 tatagtagca ataatcgttt cttcatttag tgcatatata cctgttaaaa aagctagcca
    12481 tttgccgata aaaaatattg tactaggaac agtagaagaa aagaatgttt caaatcgttc
    12541 tatacttttt ataggaagta taatgtttat tttatctatt ttacttccaa gaatatcccc
    12601 tgaaaataca ttgtatctag ctggtggatt ttcattacta ggattaattg tagctactat
    12661 tgtgttgatt cctcttatta ctgatatcat gtctatagtt tttgagtttg tttataaaaa
    12721 tattttaggg aatgagggta aattagctgc tagaaatatg aaaaataaca aaaatataat
    12781 acagaatatc actttactat ttataagtat ttctgcagtt attgcaataa gtgttgttgg
    12841 taactttgtc aaaacttata taacagatgt gtttcgtgac gcagaattac aaggttttgc
    12901 agatggaaat atgaatgaag agtttattga agatgttaga catatggaag gaattaaaaa
    12961 aatattacct ctttatgtta tgaataatga gatttctggt aatggtgtaa cattatcaag
    13021 actagagggt acagataata taaaactata taactctatg tttggaatca attatacaaa
    13081 ttttgagata aaaaaacaag taatagaagc ttttaatgat aaacgttctg ttattttgaa
    13141 tgaagatact ctaaaaaaag taggcttatc tataggagat acaataactc tatctaatga
    13201 taagtatgat ttttcttata aaatagtagg tagttttaaa tcccgtgcaa atgatgtaga
    13261 agctgttata ccatcacatt atgcagttaa tgattttgat aaaactaatt atggtttttt
    13321 agtatataca gcagtcaatc cagatgctgt tatgattcag ataagatatt tatttggaga
    13381 tacatataat tggagtcgta cagttgaaga atttaataat gattctttaa atactataag
    13441 tagttttttg tctcctatga ataagatgac ctatttcatt tttcttcttg ctacagtagg
    13501 tattattaat aatctactga ttaattatat tcaaaaaaga cgttctatag ctatgtataa
    13561 atctatagga ctaagtaata aacaaaatat aaaagtaaca ttgattgaag gggttacttc
    13621 aggtttactt ggagctgtta ttggtattgt gatatctata cttgagattc aaactatctt
    13681 tatagttgca ggaccaaaaa tttcaatgaa accagattta gattttaaaa catttattat
    13741 tgttgggtta ttgggaatta tagtaacttt aataggttcg atagtgccta ttataaaagg
    13801 gaagaaaatg aaactgatag aagaaattaa gtttgaataa ttatggaggt taatatgaaa
    13861 attacacata atagtttagc tgtagaagca aaaaatatta tcaaagaata taaaattgga
    13921 aatacaacaa ctcgagtgtt aaaagaggta tctttacaag taatgaaagg tgaatttgtt
    13981 tctatcatgg gtcaatctgg ttctggtaaa agtactcttt tatatatttt aggtggttta
    14041 gatactccaa cgagcggaaa agtgtatatg aatggagctg atatatcaca ttttaatgat
    14101 gagaaaatga gcataatacg tcgtagaaat ataggattcg tatttcaatt ttataactta
    14161 ataccaaatt taaatgtgga ggaaaatata atgcttcctt tgttattaga tggtaaaaat
    14221 ttaaaagatt ataaaaatca attagatgaa atcttagata tagttggttt aactgataga
    14281 cgtaaacata caccacgaga gttatctggt ggtcagcaac aacgtgttgc tatagctcga
    14341 gctttaattg gaaaaccaga gattctattt gctgatgagc ctactggaaa tttagatagt
    14401 aaaacaggga tagaaataat agatttactg aataaaataa acagagacaa tggacaaact
    14461 ataattatgg taactcactc accagaagca gccaaaagta gtagtcgtac tattacagtt
    14521 tcagatggtt taattgtata aggactaatt attgtatttg agataagcat ttttaattat
    14581 ataaattaga catatgtaaa gtggatataa gttaaatact ataaatgtgc tataatatta
    14641 aaataacaga taaaagtact atatttttta ttgattattt taattttatt tgtttattaa
    14701 atatctaatg agtattcata ctatctctat tattaaaagc agttaatgat atatagagat
    14761 aatatggatt cttcttactt taataaatat tcacgatagg acaggatata atatatgtta
    14821 aaaaaagaaa gtattcattt tataaataaa aataggtcac ttatagtttg tgaagacaat
    14881 tttgtttatc tcttgccttg tatacaatta agaaattgga tatcgaattt tactatatcg
    14941 tttccaaatc aaacaataat ttctgataat tacacgatta ttcctcatgg aagtgtaact
    15001 ttggtgtttt tttatgatgc cacagggtta catagtctat tatttggtcc aactactaaa
    15061 ccaaaaacag taggaaatat tgctaataga tgtgatgtta tactaattat agaatttcaa
    15121 cctgctggtt ttttcccgct tataggtata cagcaaagtg aacttattga taaagttgtt
    15181 ccattttcta taattaatac ttcattagat ttagaaatta aaaaaatatt taatgaatct
    15241 ttatctatag ataaattgat tttaaagttg gaagaattat tgatttctaa tattaaaatt
    15301 gaatactcat atgagtttat tttggcaatt cagttgatta ttcaaaactc tggaaatata
    15361 tcttcacaag aaatttctaa aaaagtattt tatagttcta ggcatttaaa cagactcttt
    15421 aatcagtatc tagggttgag tatgaagtca ttttctcgtt tggtacgtat taataaatct
    15481 ataaaacttc taaacaataa taaaacttca tggatgtcta tatgtaatga attaggattc
    15541 tatgatatac cacattttat taaagatttt aaaattgtct gtggaataac tcctcaaaaa
    15601 taccgagcta atatgtctga tttttacagt gaaattgcaa aattttaagt tataatactt
    15661 ataacaaaga aaggatagtg ataagatgaa atttggagga gtattgctaa ttgtcaaaga
    15721 cataaataaa tcaaaaaagt tttttgaaga tgttatggag caaaaaatca cattagattt
    15781 aggagaacat gtatcatttg aaaatggatt tggattacaa agtaattata aagctataat
    15841 tggatatgat ttaccaatat atcaacaatc aaataacttt cagttgtatt ttgaggttaa
    15901 tgacatcaaa caatgggaaa gtaagataaa tagaatagga aatattgaat ttttgcatta
    15961 tataaaagaa tacccatggg gtcaaagaac ttttaggttt tatgattttg ataaaaatat
    16021 tatagaaatt tcagaaagta tggaaagcgt cataaagagg cttttaaaac aaggtttagc
    16081 tttagaagaa atttcaaaac gcactatgta tccagttgaa tttattacac aatttcaaca
    16141 agtataaaat attaaaatat ataatttttt ctttatattg caaaaaacaa aggcttgggg
    16201 cataatagaa ccagaaaaac ctgaatctca acagcaaatg gctcatatgg atttcgctgt
    16261 taaggattta gaagaagcag ttcaatatgc aattcattgt ggagctacaa tcgcagagga
    16321 gcaatttact gatgactgga gagttatgat tgacccagct ggacaccctt tttgcttatg
    16381 tcagatgaaa tcaattatgg aaagtagcca tttttcattg ttatagacca aaatattgtt
    16441 tattaattac aaaaaagcac aacagtatat accgttgtgc ttttacttat tatacatgaa
    16501 tacactaaaa aatataaata gtagagcttt atcaataatg ataattttta aaaaatagta
    16561 tttttttatt gaataatttc ttagatattt taaagtatat aaattttgca tatatgaatt
    16621 ggacattatc aaatgtgtta taatattaaa acaacaaata aaattattat attttttaga
    16681 ataataattt tatttgttta ctaagtttat gatagggtat ttatttaagg taataaaata
    16741 ttatataaaa tattttaaag ccttaggtaa tattaggtag aagttttagt aaaatatgca
    16801 aaaactaatt gattatgtac cttgtatggg tatataatag atattgtttt aataaaatat
    16861 ttgaaaggga atgatttgat gaaaattact ttaccagaaa cagctattga tactttaaaa
    16921 agtatcttaa aagacaatca agataaacct aataatataa gagtatactt tgcaggtgtt
    16981 ggttgtggag gtccttcttt tggattagct ttagatgaga aaaaagaaga tgacttaact
    17041 tatgaagttg gagaattaca atttgtaatg agctctgatg aatatagtca gtatggagat
    17101 ataataatag aagatacagg atttggattt agagttattc ctgaaaatat gaaagatcaa
    17161 ggtggaggag gatgctctgg ttgttcaggg tgccactaag attaagtttt tagtaagttt
    17221 agaactctct ttatagaaat aaatttctat tttaagagag ttctttttat tttttggtga
    17281 gataatgtat aataaaaatg tattttacta attttttata tgtaaggaga attattgtga
    17341 gaagaagacg aaaaaaaggt gcagatgaaa agcttttaag ttatacaaaa tatgttttaa
    17401 gagacgatat agacaaatta aaaggcaagt ggaatttaaa gtttagaaat gataatccaa
    17461 tacatgttga atttgggact ggaaaaggta agtttataac aactttagct aagcagaacc
    17521 ctgacataaa ttatattgcc atggaactta aggaagaagt tcttttaaaa gctgttgaaa
    17581 aagcagatgc ttctaatttg aataatatac tatttttatg gggagatgta agcaatattt
    17641 tggattactt tgaagctaaa gaattatcga gaatttatat aaatttctgt gacccatggc
    17701 ctaaaaatag atggagtaaa agaagactca ctcattctgg atttttagaa atgtacaata
    17761 gggttttgga agatgatggc gaaattcact ttaaaacaga taatgaaaaa ttattcgagt
    17821 ttagtttaaa tgaaattgct gcaaataatt ggctattaaa gaatatttct cttgatttag
    17881 gaaatagtga gtatgaaaat aatgttacaa cagagtatga agacaaattt atgtcacaag
    17941 gtatgcgaat atttagatgt gaagctaaaa aacgtaacta gtaatatgtt tataagttta
    18001 atagtagaaa tttattttaa tttatattaa taaagtgtac gaaagtaggt atatataaca
    18061 aggaaacaga actctctcat agaaattaaa ttattataat aaaaaattta tgtgggaggg
    18121 tactatgaat ttagctaatt taataccatt tattatttct gcagcagctt taagtccaat
    18181 aaattcaaat gataaggttt tgagcgaaaa tcaacttaaa gatatttttc ctgatgcaaa
    18241 tttaagagca gttgtaaaaa gatatataaa tccagatgag atgactataa gcaatataaa
    18301 ggctttagat ggagaatttt atgcaactgg tgagagtatt tcaaatttaa aaggtatatc
    18361 atatcttgaa aatgtagaca attttatttt ttggaataat aatataaaag aggttcctaa
    18421 ggaagcttta agtctaaaag atatggattc aataaatctt gccaataatt atttgattga
    18481 tgacgatgta gtaaattctc tttctcataa tggtgtggat gtaaattgcg atttaaattt
    18541 tattgataca aaagataatc agtataaact ggactctaaa tatcataatg ttgatatagc
    18601 aaaaggtgaa agtattgatt tgagaaaaat aattacgaag aaaattgata gttattataa
    18661 atactgggaa gttactgata accttccaaa agatttaaat tttattgtat ctgtaagtga
    18721 taagagtgta ctttcatgtg aagatatgat aattaaaggt aataaaacag gtaaagcagt
    18781 ggtaaaagtt atactaagcg acaaaaatga tttaaataca tctcaagaag ttctaattac
    18841 tgtcaatgta aaataatctg tcaacttatt caatcattct attttcaatt taagtgtata
    18901 atcaaaataa aggttgttag taacagaacg ctaatattag tgatttgtta ctagcaacct
    18961 ttaaatttta tagaaataga ttatctaaaa aattccattt cttctgttag tagcccatat
    19021 tcctattaaa attatcagtg aatatattat atacaaaact attcttatat attggacatc
    19081 taatccaaaa aattcaatgt tactctgtga ggtatgcaac atcacaagga ttgcagatat
    19141 accaataagc aaaggattga ttactatttg cttatcagtt cttaaaatca ttattttacc
    19201 catccaaatt attgataata taaaaaataa aattttaaga ccaaattcta atgtatctac
    19261 caaagcgaaa acatcccttc atttataata aatttttcag ctcaacaaaa tataaaaccc
    19321 tacaaacaac ttttgaatcc ataataagat aacttgatta agcactatga aataagtact
    19381 ataaaacttc atctacatta atatatacta cactatactt caaatttctt aaacataaat
    19441 taatgaaatt aacaaaattt tattcagaaa catataaaga tatattttac tatttaaatg
    19501 catatcatta tatatacagt tttaggaggt gaggtatatg gaatctaaat tttttaattg
    19561 gtacactcaa tccttagggg gaacttttgg cattatatca tgtatatgcg catatttaaa
    19621 tggatacatg tttgtttata caaatagtgc tagcaaatat tttgaaagta taggattttt
    19681 gggaataatt tcaagttata cactacttcc actttgtatg cttactttga tttttggagt
    19741 tatatattct tatactccag aaacatccga caaaagtatt tttaatatac ctttttttac
    19801 tttaaataaa agattaatac ttattacagt agtttttggt tttataggtg ctagagttta
    19861 ctttataatt cctgcaattc taataacttt taacctatat agcaatgttt tgatagctaa
    19921 attaaagtac atcctatgta aaaataaaga aaatgatact aatttgaaag aaagcgaagt
    19981 ctaaattgca taatattggt atttgtctta aaaattcccg aggtattaat ttgtattctc
    20041 ttgtattttg caaaaaatat tgaattttat tcattttata cttataattt atattaataa
    20101 tggaaaatta aaacaaatat aagttactgg ttgttaattt aaaattaaaa gatatattga
    20161 tgtgttgatt ctctagtcag cactttttct tttataaatt tgttttttgt gatataatat
    20221 tagcaccagg taataataca agggggtaac aaaatgaaca tcaatgaaat taaggaatta
    20281 ttaaaggcca tagactcaac taacttagaa tatgtaaaac ttgaaagtag cgatttaaga
    20341 ttagaagtat ctaaaaaagc tcaatctaca agttcacctg ttttaagtgt acaacaagaa
    20401 tcagtagtgg atctttcttt agaaaagcca gttgtaaacg atacacctgt cacatctaat
    20461 gagaatctat cagtagtagt agctcctttg atgggaacat tctatgattc tccaagtcca
    20521 gatgcagata gctttgttaa agttggcgat gtagttgaag aaggcgacac tttatgtata
    20581 ttagaagcaa tgaagcttat gaatgagata acaagtgaaa ttaaaggtga aataattgaa
    20641 gtattagtta gtaatgaaga actagtagaa tataatcaac ctttatttaa gataaaacca
    20701 ttgtagtagg aggtatttaa gttgataaaa aaaatactag ttgcaaatag aggcgatata
    20761 gcagttagaa tcatcagaac atgtaaagaa ctaggtataa aaactgttgc tatctattct
    20821 gaaatagata aagattgttt tcatagatat atagctgatg aatctatttg tataggacca
    20881 aataatataa gtaagagtta taataatata gaaaatataa tctatcttgc tcttaaatta
    20941 aagtgtgatg ctattcatcc aggctttgga tttttatcag agaatcctga atttgcaaag
    21001 caatgtgaag ataataatat tatttttata ggtccaacaa gagaacaaat gattttgatg
    21061 ggagataaat ctagagcaag agaaactatg atggaattga atatccctgt tgttcctggt
    21121 tcagaatctg ttttgaaaac taaggaagaa gctttagaag ttgcaagaga aattggatat
    21181 cctgttatga taaaagcctc aagtggaggc ggtggaaaag gtatgagaat cgttagaaaa
    21241 gaagaagaac ttttttcaaa ttttgatatg gcaagttcag aagctctagc agcattttct
    21301 aatagtgatt tatatatgga aaagtttata gaaaatccta gacatataga agtacaagta
    21361 tttggagata aacattcaaa tgcaatacat ttaggtgata gagattgttc tatgcaaagg
    21421 cgaaatcaaa aagtaataga agaatcttta agtccatacc tttcagatga agaaagacaa
    21481 aaacttcata aaattgcagt agatatagta aaaggagtag gttatatcgg tgcaggtact
    21541 attgagttta tagttgacaa agataaaaac ttttatttta tagagatgaa tacaagaata
    21601 caagttgaac atccagttac agagatggta acaaatcttg accttataaa acttcaaata
    21661 tctatagcta atggggataa aattcctttt aaacaagaag atattacttt tagaggtcat
    21721 gctattgaat gtagaataaa tgcagaagat tcatcaaaga attttgctcc atcaccaggt
    21781 aagatagaat cattaaattt acctggaggt tttggtgtta gatttgatac ttttgtatat
    21841 gcaggatata ctataccacc tttatatgat tctatgatag gtaagttaat atgttgggct
    21901 gaaactagag aagaatgtat aagtagaata tatagagctc tagacgaaat tattgtagaa
    21961 ggtattaata caaatgtaga atttcaaaag gcacttgtaa caagtgagga gtttagagaa
    22021 gatactcatc ataccaaatt tatagaagat gtctttatga agaaagaatt tgctacatta
    22081 taaggagtaa atagattatg ataaaaaaat ttttatcatc aaaagaatct aagtatgtta
    22141 cgatttcttt agatgacaac tttaaaaaga atagtgtaga tgataaattt tggacctatt
    22201 gcaaaggttg tgatagtcat gtatttagaa aggacataga agaaaattca tttgtatgtc
    22261 caaaatgttc aagacattat ggcttaagag caagaaaaag aataaattta ttgattgata
    22321 aaggaacttt tatggagttt aattctgatg tagaatttca aaatccacta aattttccta
    22381 aatacaaaga aaaagtagac tcttataagg aaaaaacaaa agagtcagaa gctgttgtca
    22441 caggttatgg aagaataaat ggaattaaaa cagttatttg tgtaatgaat cctgatttta
    22501 tgatgggaag tatgggttct attgttgggg aaaaaataac ttattcaata gagtatgctg
    22561 ctgaaaataa tttacctata attatttgtt ctgcttcagg aggagctaga atgcaagaag
    22621 gtatggtatc tcttatgcag atggcaaaga cttctcaagc actttctaag cttgaagaaa
    22681 aatcattgcc atatatatca gttctaactg accctactac tggaggagta actgctagtt
    22741 ttgctatgct tggtgatatt ataatttctg agccaaatac gttgataggt tttgcaggtc
    22801 caagagtaat agagcaaaca ataaatcaaa aacttcctga aggatttcaa acttctgagt
    22861 ttttacttga aaaaggtttt attgacatga tagtagatag aagaaaaatg aaggaagtat
    22921 tatatcaaat tcttgctatg cacaagaaat agggcataaa ggaggaactg ttttgattga
    22981 aaataatagt gaaaaaataa aagtcttaga aaatgatatt aaacaattaa taactatttc
    23041 taaggcaaat aatatagatt taagtgataa aataaattca cttaatgaga aattggaaaa
    23101 gcttaaggaa gatgcttttt cacatctatc agcatatgaa aaggtaacat taagtagaga
    23161 tataaagaga cctacgacat tggaatacat agaacatatt tgttctaatt ttttagaact
    23221 tcatggagat aggcttttta aagatgaccc ttctatggtt ggaggaattg gtcagatagg
    23281 aaagtttaat gttactatag ttggacacca aaaaggaaga gatacaaaag aaaatataaa
    23341 aagaaatttt ggaatgcctc atccagaagg atacagaaaa gcattaagac ttatgaaaca
    23401 agctgaaaaa tttaatagac ctataataac gtttattgat acatcaggag ctttttgtgg
    23461 tctagaagca gaagaaagag gacaaggaga agctattgca agaaacttac ttgaaatgag
    23521 taaactttct gttccagtaa tcacttttgt aattggtgaa ggtggtagtg gaggagcttt
    23581 aggtatcgga gtaggaaatg atgtatgtat gttagaacat tcagtttatt ctgtgatttc
    23641 tccagaaggg ctttctagta ttctatttaa agattcatct aaagctaaag aagcttgtga
    23701 tgttatgaag cttacaagta atgatttata tgatttaaaa ataatagata agattataaa
    23761 agaacctctt ggaggagcac aaaaagatgt agaagctgtt tcaaaagaaa taaaagctta
    23821 cattttagaa agattaaatc attacaaaga tatggataaa gaggaaatta tagctcaaag
    23881 atataaaaaa tttagaaata ttggaaaatg cttatagaat ataaagaaat ttgtagtttt
    23941 ttcataataa aatggttaaa aaaaataaag gttttcttaa aacgattata gaagtatatt
    24001 tttataaaag tatactttaa atgatgattg taattagtaa atggcaatta tgatttagta
    24061 ctttaggtaa tggatacttt aacaaattaa caaggagatg ttgaaatgga ggtactaaaa
    24121 gtttcatcaa aatctaatcc taattctgta gctggagcct tggctggagt actaagagaa
    24181 cgaggcgttg cagaaataca agctatagga gctggagctc taaatcaagc gataaaatca
    24241 attgcaatag caagaggttt tgttgcacct agtggaatgg atttagtttg cattcccgca
    24301 tttactgata tagaaatcga aggtgataag aaaacagcca ttaaacttat aattgaacct
    24361 agataatata tctctttatt agaatttaat taagttttaa aggttggcta tagaaatagg
    24421 acactatagt gaaccttttt tactttatat gttttatttt atgtaacttt taatgtataa
    24481 tcttaagtaa acaaataaga ttggaggaaa tatacattat gaaattaaaa ttattggtag
    24541 aagagtatgc agtatgtaga ttaaataatg acagtaaaat accaacatgg atagatacag
    24601 agaagtttta ctctataact agaactgatg atgaattaag tgttgtatgt ttaaataata
    24661 atattccttc tgatgttaag tcagaaaagg agtggagaat acttaagata ctaggtcctt
    24721 tagacttttc acttgtagga attttatcta agttaagtag tttgttagca gacaacaaaa
    24781 taagcatatt tgcaatttct acttatgata cagactatat cttagtaaaa gaaaaggata
    24841 tagaaaatgc ttgtaaaatt ttaagctgta atggatatga agtagagtag ttaaatactt
    24901 ttacatgagg atttaactta tgatttatat aatcgaaatt ttaaaaataa gtgtaataaa
    24961 tattttttgt ttcataggaa tatttattgt atttggattg atttttagca ttacagaaaa
    25021 tctaaataat aaatttatat tttcatcttt tggaaaaact ggaattattg taactggagc
    25081 tataggaaca tttgttcatg aattgagtca tttgataatg tgtttaattt tcatgcataa
    25141 aataaattca gttaagtttt tcagaccaat agaaagtaaa aatgatggta tactaggata
    25201 tgtaagtcat agctataaaa aagataatct ttatcaaagt ataggaaatt tctttatagg
    25261 aattgcacct attataggtg gaacaataat gattatttta atttttaaga tattactgcc
    25321 agatagttat atgaaagtga ctcaaaatat agatttaaag ttatatgtaa gcatgattaa
    25381 taatttaaat ataaatggtt ttatacacat tattttaggt gatatttcca attttataag
    25441 aataatgttg ttaacaccta gtgtatattc attgaagtat tttatattta tgtttttaat
    25501 gtattctata tctacacata tgtcacttag tagtgctgat ttaaaaaata gcttaaatgg
    25561 cctgagcttt atagttatag ttatttttgt aataagttca attacatact tatttggatt
    25621 tgataacttg aatatatcca gtttaatagt taagtataac atatttataa gtttctttat
    25681 ggcaataggg tttatttttt caatagtaac tctcataata tcctatattt taagcttagt
    25741 cagcccattt aaaaacttta g
//
LOCUS       DAYASV010000047        25752 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYASV010000047 DAYASV010000000
VERSION     DAYASV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.047"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            746..1159
                     /locus_tag="ACGI68_001877"
     CDS             746..1159
                     /locus_tag="ACGI68_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138318.1"
                     /translation="MSKSELGVKTNLVSELLMISYFFIDSLLKKFIFKEGNLCITFII
                     LFAFWACDFMYNKKYNDLVDEASKLMLLKVDHIASKIMLYSILLIAVFFSANYTRGLD
                     ISNLDIGVILLAILFIQSLSKLILYIHFDRKGICN"
     gene            1227..1643
                     /locus_tag="ACGI68_001878"
     CDS             1227..1643
                     /locus_tag="ACGI68_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138319.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            1644..1856
                     /locus_tag="ACGI68_001879"
     CDS             1644..1856
                     /locus_tag="ACGI68_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138320.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            2253..2423
                     /locus_tag="ACGI68_001880"
     CDS             2253..2423
                     /locus_tag="ACGI68_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138321.1"
                     /translation="MSALLYGVCNKSISVSIVINMQEFKIGVSMAYGVLVIGICYIAL
                     GIIFKLDRKRFT"
     gene            2966..3844
                     /locus_tag="ACGI68_001881"
     CDS             2966..3844
                     /locus_tag="ACGI68_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFH0138322.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKAAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            3880..5775
                     /locus_tag="ACGI68_001882"
     CDS             3880..5775
                     /locus_tag="ACGI68_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFH0138323.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            6043..7950
                     /locus_tag="ACGI68_001883"
     CDS             6043..7950
                     /locus_tag="ACGI68_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138324.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVTTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            7940..8389
                     /locus_tag="ACGI68_001884"
     CDS             7940..8389
                     /locus_tag="ACGI68_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138325.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNAHTKKEILDIFYEV"
     gene            8489..9376
                     /locus_tag="ACGI68_001885"
     CDS             8489..9376
                     /locus_tag="ACGI68_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0138326.1"
                     /translation="MAIYLEMPKLIHEFPFRTLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            9781..11229
                     /locus_tag="ACGI68_001886"
     CDS             9781..11229
                     /locus_tag="ACGI68_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFH0138327.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKN
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MSYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMILGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            11255..13645
                     /locus_tag="ACGI68_001887"
     CDS             11255..13645
                     /locus_tag="ACGI68_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFH0138328.1"
                     /translation="MSLIFTTKDQLVELGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHTRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVDDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSAGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGVRTYFEATNDEEFMMQNGLEILIETSRFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVFVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            13647..14318
                     /gene="pgmB"
                     /locus_tag="ACGI68_001888"
     CDS             13647..14318
                     /gene="pgmB"
                     /locus_tag="ACGI68_001888"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFH0138329.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     INREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGVKDEFDCIVNPSLLKRGKPYPDIFIEGSKMLNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            14357..15727
                     /locus_tag="ACGI68_001889"
     CDS             14357..15727
                     /locus_tag="ACGI68_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0138330.1"
                     /translation="MKNKYEDVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQGVIYAASV
                     IPALLTVWMMSYIENFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            16074..16916
                     /gene="licT"
                     /locus_tag="ACGI68_001890"
     CDS             16074..16916
                     /gene="licT"
                     /locus_tag="ACGI68_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFH0138331.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            17061..18989
                     /locus_tag="ACGI68_001891"
     CDS             17061..18989
                     /locus_tag="ACGI68_001891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0138332.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVDAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            19050..20477
                     /locus_tag="ACGI68_001892"
     CDS             19050..20477
                     /locus_tag="ACGI68_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFH0138333.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFVVENGLGAKDIADENGYVEDDYR
                     IDYIREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            20888..21664
                     /locus_tag="ACGI68_001893"
     CDS             20888..21664
                     /locus_tag="ACGI68_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0138334.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            21704..22045
                     /locus_tag="ACGI68_001894"
     CDS             21704..22045
                     /locus_tag="ACGI68_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFH0138335.1"
                     /translation="MQEITSKFKECRKAISAMGDETRQLILIALLESDFNGIRVGEIT
                     KKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWRSLTELINLIFVGIQ
                     HISENDIRKGD"
     gene            complement(22221..22970)
                     /locus_tag="ACGI68_001895"
     CDS             complement(22221..22970)
                     /locus_tag="ACGI68_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HFH0138336.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELIST
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFEIITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            23192..23320
                     /locus_tag="ACGI68_001896"
     CDS             23192..23320
                     /locus_tag="ACGI68_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138337.1"
                     /translation="MKDYKYKGYFEGVEIFDNVNDLNLNTLTTVSRFVFVFLFTIT"
     gene            23448..23630
                     /locus_tag="ACGI68_001897"
                     /pseudo
     CDS             23448..23630
                     /locus_tag="ACGI68_001897"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            23658..23873
                     /locus_tag="ACGI68_001898"
     CDS             23658..23873
                     /locus_tag="ACGI68_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0138338.1"
                     /translation="MVPPKGKFFFGAVVVGERGQIVIPKEAREVFNINAGDKLLVLGD
                     EERGLGIVHQRDLINFIGHVGVVHKEE"
     gene            23905..25092
                     /locus_tag="ACGI68_001899"
     CDS             23905..25092
                     /locus_tag="ACGI68_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HFH0138339.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            25284..25688
                     /locus_tag="ACGI68_001900"
     CDS             25284..25688
                     /locus_tag="ACGI68_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFH0138340.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPPIYLKYKPDK"
BASE COUNT        10229 a         2649 c         4456 g         8418 t
ORIGIN      
        1 ttctaatata gatttctttt tggtgaattt aaattttatt taaatttaaa ttctaaacca
       61 ctttctatat agccaacaga aaagtgttaa aaaattatca tatatataat tactacttat
      121 agtaacacaa atagatctaa taactcctat aataaaattt atcattagtt acatatagaa
      181 taaacctcag ttttgattac tgtaggaact aggattatat gaagctacca ctcatagatt
      241 tcagtttttt ctgttgtatc atagcataga atagtaaatt tatctgaaac tttattttaa
      301 atttagaatt tgcatataga taaattatgg gcattattct gatgattcgt ctccaataat
      361 taaaaaaaga atgataaaat acttgaaaat actagtcttt attgactagt gtttttttat
      421 ttcagtgata aaatatacca attatacttc agttacagga aatgatgttt tgagggtaca
      481 ttgggaggca tttaggttgt ataattataa gtttcaatgt tatatgctca ttagaataat
      541 agttagtaaa tgatatgatt gagatgatag atgaaataaa ctaatttagt actgtattgt
      601 aatgtttatt ataaaatctt ttataaaata tatttatgaa gatattattg aatattttca
      661 atattctatt gcaatttatt tggaaaagtt atatatttat aacataaggt gttataaata
      721 tataactttt aaggggtgat tttatatgtc taaatcagag cttggtgtta aaacaaatct
      781 tgtttcagag ttacttatga tatcctattt ttttatagac tcattattaa aaaagtttat
      841 ttttaaagag ggaaatcttt gtattacttt tataatttta ttcgcttttt gggcatgtga
      901 cttcatgtat aataaaaaat ataatgattt agttgacgag gcgtcaaaac ttatgttatt
      961 aaaggttgat catattgcat caaaaataat gttatattct attttgctaa tagctgtttt
     1021 cttttcagca aattatacaa gaggtttgga tatatcaaat ttggatatag gtgtaattct
     1081 cttagctatt ttatttatac aatctttatc taaattaatt ttgtatatac attttgatag
     1141 aaagggaata tgcaactaag tgataatatt gataaaaacg aaaattaaag agtatagaga
     1201 atataatttt taaggagtga ttttatatgt ctaaatcaga aactggtgta agatcaaagc
     1261 ttatcctgga gttgcttgta ataacttatt tttttatagc tgtattagcc actaagtata
     1321 tttttaaagg tccaagcact gcttctcttc cagggttgag ttggttaata gtttttggat
     1381 gttattcaat ttataataaa aaatataatg atttagttga tgaatcatct gaatttatac
     1441 tatcaaaggt taataagatt gcaataaaaa taatgatata ttctatagta ataatatcta
     1501 ttttctttgc aacaccttat acaagtaata taaatatatc aaacctagat acaggtataa
     1561 tcctcattgc agttttattt atacagtctt tatctaaatt agtgttgtat atatactttg
     1621 ataaaagggg gatatgtaac taagtggcaa tattgatgaa aacaaaaatt aaagaataca
     1681 gagaaaaact ccttatgacg caaaatgaac ttgctaaatt ggttggggta agacgtgaga
     1741 ctattgtaca cttagaaaat gggaaatata atccttcttt gaagttagct atggatatcg
     1801 ctaaagtatt cgatactaca gtagaaaatt tgtttgaatt tattgaagaa gaatagtgga
     1861 aagtttataa acatttataa gatggataat aaaatatagt tatttaatta ttaagtttgt
     1921 taaaaatatt tatataatat aatatatgat taaggtcaga ttataattgt tttaagataa
     1981 aatgtaataa aatttaatgg agaaatttta taggtagata aattttaata tttataagat
     2041 tatgttaagg aaacacttaa aaattagatt aaaattgtgg gtatttaaat caactaaaag
     2101 atattgacta tttagtcaat atcttttttt attaaaaagg aatatacata taaataggtt
     2161 agattaaaaa ttgggataaa tatgatttat atagcatcat ttaattaaaa ggagctatat
     2221 tagtattcat tcatacaatt ggataatata caatgtcagc cttgctttat ggtgtttgta
     2281 ataaatctat ttcagtttct attgtaatta atatgcaaga atttaagata ggagtatcta
     2341 tggcttatgg tgtattagta attggtatat gctatatagc attaggaata atttttaaat
     2401 tagatagaaa aagatttaca taagataaca aaggctaatt tataaattgg tgcttagaaa
     2461 accaaatcac tttattagct agagatgaat ttggagggtg tcactttggt gttttatgta
     2521 aagtttatgg aagtaaaaca ttagataatc gtgagtaaga tttctataaa tcgtatatac
     2581 tattggaatc caagaaaagg tattggccaa atagctaata tctttttaat ttttttgact
     2641 ctaatatagc attaactatt gcatgagtat ctttaaattt atagttaatg tatcttaatt
     2701 gtttattata aaatctagga aggctaaagc ataaaacact taaaaagaga agatgtacat
     2761 gagaaatata attaactatg atgcagactt ctaaaaatta tttagtgaaa aggaacttag
     2821 agtgtataat aaaagttcct tttcactaag gaaaacgatt gttattttct gacattcatt
     2881 accctgatat tatttaaata taaacaacaa caaaattaac aagaagcaaa ttcaacctaa
     2941 aatttaaaat atcgggaggt acaatatgtt aataaacatg aaggaaatgc taaaagtggc
     3001 acaagaaaat caatttgctg taccagcatt taatatagga agtgggcaaa tacttaaagc
     3061 agtagtacag agtgctaacg aaaaaaatgc accagtaata cttgcaatac acccaaatga
     3121 gctaagtttt ctaggagata gctttgtagc tagttgcata gaagaagcaa ataagagtaa
     3181 agtacctatg gtaatacact tagatcatgg ggaaaataaa gaacaaatat taagggcaat
     3241 aagatgtgga tttacatcag taatgattga tggctcacat ttaccatatg aagagaatgt
     3301 agctatatct agggaaatag tagaaatagc aaaaggatta aatgtgtccg tagaaggaga
     3361 acttggaaca ataggaacaa caggaacatc aagtgaaggt ggtactgatg aaataatata
     3421 tacagattca aaattagcta aggattttgt tgaaaaaaca ggagtagaca cactagctat
     3481 tgcaattggt acagcacatg gtatatatcc aaaaggtttt aaaccagagc ttaaactaga
     3541 tttattaaaa gaaataagag aagtagtaga tataccttta gttcttcatg gtggttcatc
     3601 aaatccagat gaagaaatag cacaagcagt taaactagga gtttgtaaaa taaatatttc
     3661 gagtgatgtt aagtcggcat attataagaa gtgcagagaa ctattagaac aaaacccaag
     3721 cttatatgag ccagatacta tatatccacc atgtataaaa gctgcaagag aagttataga
     3781 gtttaaaatg aatctattca atgctataga taagttaaag tgcttttata aaaataaaaa
     3841 ttaattatga ggtaacaaat taaaaggagg ggagaattta tgataggtaa aatactacga
     3901 agcgattgta ttgtactaga taaacaatta caaacaaaaa aagatgtgat taattatatg
     3961 gcaaatcatt tgtaccatgg aaattatata aataataaag aaaagtttat agctgatatt
     4021 aataaacgtg aaaaagaatc atcaactaat atgggtaatt taatagctat acctcatgct
     4081 aagtcaaatg tagtaaatca acctgtaatg atgtatttaa gaacagatag agagattaca
     4141 gataatgatg gtcaaaaact taggtcattt tttatgatag cagttccaga atcaggttca
     4201 gatgaacatc ttaagattat atctaaacta gctacatatc taatggaaga aaattttctt
     4261 aagcatctac taagtgcaga aactccatac gaaatattaa gtatatttaa taataaagct
     4321 gcagaatcag gagattttag taataatcaa aataaaggtc ttatagttgg gataacagga
     4381 tgtccaacag gtatagctca tacttttatg gcaaaaaaag cattagagga agcagggcaa
     4441 gaacttggat atgaagttaa aatagaaact catggagcta taggtgtaga aaataagttg
     4501 actaaagaag atataaaaag agctgaatac gtaataatag cttgtgataa agaagttcaa
     4561 ctgagtagat ttataggaaa gaaagtgcta caaaagaaag tagtagatgc taagaatgtt
     4621 tcattagcta aaaatttaat tataaacgct gaaaatggaa tggctaaaga aattaaacca
     4681 ggtggaaaga ctttagaaga ttcaaaagaa atgcttcaag ggtcagagaa aaagggtgtt
     4741 tataagcact taatgagtgg tgtatctgaa atgattccat ttgtagtgat aggtggaatt
     4801 ggtatagcca tagcttttat gtttggtata tttgcatcag acccaactca tgaaacatac
     4861 aaccctatag ctggattctt tggacaactt ggaggcgatt cagcatttaa gttatttata
     4921 ccgatacttg ctggttttat agctaaatca atagcaggaa gacaaggatt tgctcctgct
     4981 atgatagctg gttttatggc agttattgga ggttcaggtt ttataggtgg tatggtagct
     5041 ggttttgcaa caggatatat agctttagga gtaatgaaga taacagaaaa tttaccagat
     5101 gtattaaaag gagtaaatgc tgtattgata tgtccacttt tgattgcatt tacgagtgga
     5161 gctataatgt tctttgtagt taatacgcca gcatcatggt taaatgaagc tttacaatct
     5221 tggttacaaa gtatgtctgg aactaataaa gtattattag gtgcaatatt agcagggatg
     5281 atggcatcag atatgggagg acctattaat aaaacagctt cagcttttgg attggctatg
     5341 ttttcaagta atatatttga accatcagca gcattaatgg taggaggaat ggtacctcca
     5401 ttagcaatat cattagctat gttaatattc aaaaataagt ttactaaaga agaaagaaag
     5461 tcatctctac caactgctat aatgggggca tctttcataa cagaaggcgc aataccgttt
     5521 gcagctgcag acccattaag aatgattcca gcaaatataa ttggttcttg tataggtggg
     5581 gcaatatgta tgagtttaga tataacttta atggcaccac atgggggaat atttgtaata
     5641 ccatttgcat gtagtgcacc agttgcatat atagcttgta tattgattgg tacagtaata
     5701 acagcattga ttataggggt aacaaagcct actataagga gtgaaaatag tgaagttgaa
     5761 ttacttggtg cttaaattat aaataatagt aaaaataatt aatggaataa caacaagtat
     5821 atagaataat aataaggata tttagaatca gttaactatt tctaaatatc cttaattttt
     5881 attttgaaat tatattatct attatatcaa tttagtaagg tgtatcataa attcttttat
     5941 gatacagaag tatcttagta attttacata gttagtaaat atgttaaaat agtacttaat
     6001 ataaacatag aaatatataa acataggtag ggagggctaa gtatgcttgt gtcgaggcaa
     6061 attgaatttc ttaaattgat actaaacgaa aataaatata gacctataaa atactttaaa
     6121 gataaattaa atgtatctga taaaacttta caaaaagatt tgaagatgat tgagaaatat
     6181 ttagaaactt ttaatataaa aattgatata aaaagaggct atggaatatt aatagaagat
     6241 ttagcaaaga agaacataga cttaattaat agtttaaata tacaatcgaa ggaaaataaa
     6301 tctttatcag tagagcagag aagaatggag attttaaagt atttattgct aaattcaaat
     6361 aatataactt caataaatca gttagcagat aaatattatg taagtaaaac ttctatagta
     6421 aatgatttta aatatataga ggcatggata aaagaataca atttaaaact taataaaact
     6481 ttagaaggaa ctaagattat aggtaaagaa gtggatataa ggaaatcgat tgccaaaatg
     6541 atggatgatt tattagatga aaattatgaa caacatgata tatctgagtt aacaagatta
     6601 gattctgcta catttagtgc attgataaat ttatttgata ttgattctat tatatttgta
     6661 gaaactataa ttacagaatt agaatacaat ttaggatata ctataaatca gccctactat
     6721 ataaatttaa ttacccatat acttatatgt ttaaagagaa tcgaagaagg taatcaaata
     6781 gaatctcagg aagaaagaga aatttctgta gataatttgg acgagttagt atataaaaat
     6841 gtcactacac ttatagataa gatagaaaat agatacagag taaaaataac gaaagatgaa
     6901 attatgtaca tatatatttt tttggtatca tctggattta gtagcgaaaa taatagtagt
     6961 tacaatgatg aatcagatag attaagtgaa agtgaaaaag tttcatctat aatgatagag
     7021 aatatgtcac gatttttaaa tataaatctc aaaaatgatg acttactaca gaaaagtcta
     7081 gcatctcatg taaggcctat gttaaacaga ttaagatatg atatccaaat aaaaaacccg
     7141 ttgttaggag agatacaaga gcgattttca gatgtgctgg gattatgtat gatgactata
     7201 aatattatga cagagtatga taatctgaag aatattagta tagatgaagt ttcgtatctt
     7261 gccacatatt ttcaagcggc aatagaaaga aatatgagta gtaaaagagt aatagtagtt
     7321 tgtcatacag gatatggaac atctcagctc cttgcagcca gattaaaaag agaatttcct
     7381 gaatggacta tagttgatat agttcctatg catcagttga gtaagcgaaa tttagatgat
     7441 gtagactttg taatatctac tgtaaactta gatttgaaag ataaattaca tatagttgta
     7501 tcagtactat taatggaaag tgatattaat aatataaaaa atgctttact ttctaaacct
     7561 aaaaaaagta tgaacttaga tacaaactta ttaggaatgt atttagaaga taatatatat
     7621 tttaattttg atgtagctca gatggaaagt ttaataggtg tgaatttagg gagtaaatat
     7681 gaatctattt cattaggaaa agatttaaat atatatatac ataagcaaag taaattaaat
     7741 aaagggataa tgaatatcaa taatgttact agaacgattg atatttattt agaaattagt
     7801 aatcaaagtt ttttaaagaa tattttaatg gaaatatcaa atttatatag gcaaaagaac
     7861 tatataaact atataataga ttgcaagagc aaagaggata ttaaaatatt atttaagaat
     7921 aatctattgg agaaaacaaa tggaaattag agaaatatta acagaagaaa gcattatttt
     7981 agatttgaaa tcaactacaa aagaaggcat aatacaggaa ttagtagata aattatacga
     8041 tacaggaagt ataactttaa aaagtaagtt tataaaggca ttaaaacata gagaaaaaat
     8101 aggctctact agtataggtt atcaaatggc catgccacat ggaaaaacaa aatatgtaaa
     8161 gaaggcatcc atagtaatag gtaaatctaa aaaaggtgta gattggccaa gtatagatgg
     8221 cttacctgta aaggtaataa ttatgtttgc tatgccacaa tctggaggta atgaagatat
     8281 gatattaata tctaaaatag cttacatttt aggtggtaaa aatataggtg aaagactttt
     8341 aaatgcacat acaaaaaaag aaatattaga tatattttat gaagtgtagg tattgtataa
     8401 attataaatt tgaggttttt aaaataaaat gtgtattctt ttaaatagta tagattaatt
     8461 tttagacact agagaggaga ggatatatat ggcaatatat ttagaaatgc ctaaattaat
     8521 acatgaattt ccttttcgta ctctatcaaa tgaaggagaa gtattaacaa caccacactg
     8581 gcataaggaa atagaaatat tacttataac agaaggtgta gtaaatctat ttataaatga
     8641 caagcctatg caattaaaaa aaggcgagat agtgataata aaaggaggag atattcatta
     8701 tatactgcca tcaccgggaa gtgaaagatt agtatttcaa tttgatatat ctttttttaa
     8761 tgatttaatt ttattaaatg aggagaaaga aagcttaatt aatttattca gtagtataaa
     8821 aaaatgtagt agagattggt cagcaaaaat aagaaagtca gttttcgata tattgataga
     8881 tatctacaat gaagacctat acaaaataga aggatatagt tatgcaataa aaggtaaaat
     8941 gtataattta attcctatat tatatagaca gattcctaga gaaattgaag aaaatacagt
     9001 agaatatgaa ataaactcta aagagatatt agagagattg aacaatatat tcaaatatgt
     9061 agaaaaaaat tataaacaac ctataaaatt agaagatgtg tcttatgaag taggatttag
     9121 tgtatattat tttactaaat ttttcaaaaa aaatacagga aaaacattta taactttttt
     9181 aaatgaatat agattagaaa aagcaaagtg gatattactt aataacaata tatccattgt
     9241 agatttggtt gaagaagttg gttttggaag tacaaaaact ttttacagag tatttaaaga
     9301 aaaaattgga gtctctccat tagaatttaa aaaaagatat aatagcttgg agaaaaaagg
     9361 gagtgtctca aaatgaactt agttcatgag gcactttctt ttatatgaaa tcaaatatat
     9421 tttcagtatt tcaacaatga aaattccatt catttataaa gtctaaattt tagttctcaa
     9481 tacaacctct atttcgaaat aaatcctttt atatatataa atatacagca atatatgaga
     9541 ataagataac aataatgagg atataaaaat gtatttgcta tatatataat tatatacata
     9601 gatatctaat attgatattt aatatgaaag tattgaaaat agacaatatt aattatagtg
     9661 agtaaaaaat ataattggat ttttaaattg aattatttaa aaggtatagt atgaaaaaaa
     9721 tcttataact aaatatgaga tagaaattat aaaattttat actgtgaaag aggtagatgc
     9781 atgaaattag ggaaagattt tttatgggga ggggcaattt cagccaatca aagtgaagga
     9841 ttttatttag atggaggaaa aggtttatct cagtttgata aactcccatt aaatgataag
     9901 agattaaaaa atgtatgttt agatgaggat aatattttaa atgaaaataa tcaatactat
     9961 ccaagtcata tagggattaa attttgtgaa aattacaaag aagatatagc aatgttagca
    10021 gaaataggtt ttaaatgttt tagattttct atatcttggt caagaatatt cccaacagga
    10081 gaagaaagtg aaccaaatga aaagggatta caactttatg ataatataat aaaagaatta
    10141 aaaaaatata atatagagcc attaataacg ataagccact ttgatatacc ttattctcta
    10201 gttgaaaaat attctgggtg ggctgataga agagtggtta atttatatgt aaaatatgct
    10261 gaaattttga tgaaaagata taaagatgat gtcaaatact ggattccttt taacgaaatg
    10321 aatatgataa tgcatattcc atatataggt gctgggttaa cttttatagc tgatgaaaat
    10381 caacttcaga aaaaatatca agcagcacac catcaattat tagctaatgc aaaaactata
    10441 gaaataggaa ggtatataaa taaagaattt aagtttgggt gtatgttagc tgctggaaaa
    10501 acatatccat acacatgtaa accagaagat gtatttgcaa ctttagagca tgagaggcat
    10561 aatttgtttt tttctgatgt tcaagttagg ggtgtgtatc caagctttat gagttattat
    10621 ttaaaatcaa atcaaataaa attaataata aaagaggaag atttatcaac acttaaggat
    10681 aatactgtag attttgtatc ttttagctat tattctagtg cttgtagttc agctagagct
    10741 gaaggtttag aaaaatcaaa ggcaaacgga ccagaaacca taaaaaatcc atatcttcca
    10801 aaatcaaata gtgtatggca aaacgaccct gtagggttaa gaataactat gaatcagtta
    10861 tatgaaaggt atcagttacc tttatttata gtagaaaatg gactgggtac tcaagatatt
    10921 gtaaatgaag atggaaaaat aattgatgat tatcgtatag attacatgaa agaacatatt
    10981 aaaaatatga ttcttggagt aaatgaagat ggaatagagc ttataggata tcttatgtgg
    11041 ggatgtatag atgtaaccag tgtaagtgaa ggaaagatga caaaacgata tggcataatt
    11101 catgttgatt ctgatgacta tggaagaggc tcatttaaaa gaactaaaaa acaaagtgct
    11161 ttttggtata aaaaagtaat agaaacagat ggagaaattt tatttaatga tatggaaact
    11221 catgcataat tcattttaag aaaggagtgt tattatgagt ttaatattta ctactaagga
    11281 ccaacttgta gagctagggt ttagtaagga tacaataaaa tccaatgaaa cgttgtttac
    11341 actagctaat ggacatttag gagttagagg gaatattgaa gagtctgatt ttaataatga
    11401 gtatgtagat aatatgggta catatgtaaa tggattttat gaagaatctc caattacata
    11461 tggagaaagc gcttatggct atgcaaaaca aaatcagact atctgtaagt tacctaatgc
    11521 tcatatagta aatttctcta ttgaaggaga ttgctttgat ttatcaaaag gtattacaag
    11581 tgaacatact aggatactag atttaaatga agggattctt aaacgttcat ttatatggga
    11641 aaactctcgt ggtagaaaag ttaaagtatc tatagaaaga ttagtatcac aagatataga
    11701 agaattaatg atgatttcat atacaataac accattgaat tttaatggaa aaataaaatt
    11761 taaaaataaa atggaaaata gtatggagaa gtttaattgt gaaaaagtag atgacccaag
    11821 agtggcatct ataaataaga agcaatttaa aattaatgta aaaaaatata acaataagta
    11881 ttttatgaat ttagttacag aaagaagtag attagggtta tggtgtgggt ttgattcata
    11941 tattacttgt ggaaatatta caaatgagac ctataatgtt tcagatgaat gtattgagcg
    12001 agaaatagaa gtctatgcca aaaaagatga aaggttaaat ttagaaattg ttgtaggcta
    12061 tagtagagta tattctgatt taaatctaaa tgaagaacgt ttaaaatcat tagaatgtct
    12121 ggagtcgtta aaatgtttag aatcagaagc acatttaaaa tcattagaat tggttttaag
    12181 agaagcatgt aatattggat ttgataaagt caaaaatatt cagagaaaga aaatgaatga
    12241 gttttggaca acctctgatg tagagattga aggcgataaa actcttcaac tggggataaa
    12301 aaccaattta tttcatattt atcaatcagc aggaagagat ggaaagacta atatatcagc
    12361 aaaaggtctt acaggtgatg gctatgaggg gcattatttc tgggatacag aaatgtatat
    12421 attaccattt tttatatata ctcaaccatc cattgctaga gctttattag attatagata
    12481 ctcaatctta gatgcatcaa gagaaagagc tagagaactt ggaataaata aaggttgctt
    12541 atattcatgg agaactataa atggtaatga atgcagtgca tattatccag caggaacagc
    12601 acagtttcat ataaatgcag atatagctta tggtgttaga acgtattttg aagcaactaa
    12661 tgatgaagaa tttatgatgc aaaatggctt agaaattctt atagaaacat caagattttg
    12721 ggttgaattt ggagattata tagaatcaaa aaataataaa ttctgtataa atggagtaac
    12781 aggaccagat gaatatacag caatagtaaa caataattac tacactaatt taatggctaa
    12841 gtataattta atatatgcag tacagatggt agataaattt aagcataatt tattttcaaa
    12901 tgaagtattt agaaaagtta aattacaaga atatgaagtg aaaaactgga taaatgccgc
    12961 tcaaaatatg tatttaccat atgatgaaga gaagaaaata agcaaacaag acgattcatt
    13021 tttagaaaaa tcagtatggg attttaaaaa tacaccaaaa gaaaaatatc cattacttct
    13081 ccattatcat ccattaacaa tatatagata tcaagtaaat aaacaagcag atactgtact
    13141 ttcagcgttg cttttccctg atgagtttag cttagaacaa aagaagagag attttgaata
    13201 ctatgaaaaa ataactactc atgattcatc tttgtcaagg tcaatatttg gaatgatggc
    13261 aagtgaaatt ggaaatcatg aaaaagctta taactatttt atggacacag cattaatgga
    13321 tattaataac ctacaaaaaa atacaagtga tggaatacat gctgctaata tgggtggaac
    13381 atggatgagt ttagtatatg gatttgcagg aatgaaaata aaaaatgata aattaagttt
    13441 tgaaccaagg atacctaaac actggaaaaa tattaaattt aatatattat ttagagataa
    13501 tttagttttt gtaaatatag aagaatataa atcttattat aagttaatca aaggtagaaa
    13561 tataaccata ttacatgata ataaagaaat aaaattatca aagaacataa tagaagttaa
    13621 taattgtttt aaagggagta attaatatga aagcagtctt atttgatctt gatggagtta
    13681 taacagatac tgctaagtat cattttgaag cttggcatga gatagcaaaa tcgctagata
    13741 tagaaataga tgaaactttt aatgaaaaat taaaaggcat aaacagggaa gaatcattag
    13801 ataaaatatt gttatctgct aataaagtag atagttatac taaagaggaa aagttaaagg
    13861 tgtgtgaaga aaaaaataac atttatataa agttaataaa ctcattaaca tataaagata
    13921 tattaccagg gataaatgag cttataaaag aacttaaaaa aaataatgtg aaattgggaa
    13981 tagcatctgc tagtaagaat gcacctagga tattagaatc tttgggagta aaagatgaat
    14041 ttgattgtat agtaaatcca tctttattaa aaaggggaaa accatatcct gatattttta
    14101 ttgaagggag caagatgcta aatttaaaac aatatgattg tgtaggaata gaagattctt
    14161 attcgggtat taaagctata aatgctgcca atatgacatc aattggtgtg gggaaaaaag
    14221 aagtactagg aaaatgtgac catattgtag aagatactag cttattaaat tacaatacac
    14281 tattacaagc ttggagtaaa aagattaatt tggattaaaa ttatattaaa aattatttta
    14341 aaagagagga taaggtatga agaataaata tgaagatgta gctcaattga taattgatgg
    14401 acttggagga aaagataata taattgactt aactcattgt atgacacgat taaggttcat
    14461 cttaaaagat gaaaagaaag tcaatgcaga taaattaaaa tctatagata aggtagttgg
    14521 agtaaactca acttcaactc aatatcaggt cataataggt aatgaggtag gtgttgtatt
    14581 taatgcaata ataaataagg ggataaatac cacaggagat attaaaagcg atataaaaaa
    14641 taaaaataaa aaaaatagta ttttaaataa tataatagat acaataactg gttgtatgac
    14701 acctatgata ccagccctta cagcatctgg gatgataaaa gttatattat ctctttgcac
    14761 cacttttaat tggctatcat caaattcaag tacatataga gtattagata taataggaga
    14821 tgtagcattt tactttatgc ctataatact tgcaataaat gcagctaaaa aatttaatgt
    14881 taatacatca atagcagtta tagtagtagg tgtgttttta catccaaatt tctctgcatg
    14941 ggtatcaagt ggggacccta tatcatttat aggagtacca attcaaggtg ttatatatgc
    15001 agcatctgtt atccctgcac ttttaacagt atggatgatg tcatatatag aaaatttcat
    15061 tgataaatta acacctagca tgttaaaaac aattttaaat ccaactttag tattattaat
    15121 aagtgcgcca atagcattaa tagtaattgg accaatagga aatttcttag gagagggatt
    15181 agctagtata ataaatttac tacaaggaag gttaggattt ataatggttt gtttattagc
    15241 agctgcaatg ccatttatcg ttatgacagg gatgcatcat gcattgactc ctatattcat
    15301 atcagcattt gcaactacag gacaagaatc gctaatatta atatctcaag tttgtgctaa
    15361 tttagcgcaa ggtggcgcaa cattagctgt agcattaaga agcaaagata gtaagatgaa
    15421 acagttagca tcagctgcat ggatatcagc tattatgggt ataactgaac cagctcttta
    15481 tggagttaca ttgaaactta aaaaacctgc tatgttagca tctataggag caggaatcgc
    15541 agggttattt gcaggaatta ctcatgtgac tttatatgtt ccacaaaata gtataatggc
    15601 agtacttgga ttatcagggg aaaaggggat ttcaaatata gtaaatggac ttatagttat
    15661 atttatagca ttggttgctc catttattat gacatatgtt tttggagttg aaaaaaatgt
    15721 taaataaagt acagataatg tagtagagga agtataatat taactattta cattaataga
    15781 atttttatta ttattgacta taacttgttt tgtatttata ttgatgcaaa gaaataagtt
    15841 aatattaata agaataatta ttataactga caaaaagcaa cataaaagat tgatgaagta
    15901 tatggaaata aaaagttgac aaaatatgtc tgatgtttta tcatattaat tggaattagg
    15961 tagtaattgt ttaagtacaa gctaagctta ttcattaata atagcagtat cctaggaagg
    16021 agtatgccca ttattatgga taagcttttt ttatgcttaa aaggaggata atcatgataa
    16081 ttaataagat attaaacaat aatgttgtaa tcacattaga tgataatgat gaagaagtta
    16141 tcgttatggg taaaggaatt ggttatcaaa aatcaaaagg caatctaata gataaaacta
    16201 aagtaaataa agtatttaga atatcaaata aagaaatatc aaacaaacta caagaattac
    16261 tcaataatat tcctatagaa cacatgaaat tatcaagtga aattatagaa tacgcacaaa
    16321 taaagttaaa caagaagcta aatgaaagta tttacatatc cttaagtgac catacatatt
    16381 ctgcaattca aagaatgaaa gaaggaataa atgtaaaaaa tgcaatcctt tgggaaatta
    16441 aaagatttta taaagaagag tttgaaattg gaatgaaagc actagatatt attgaaaaca
    16501 aaacaggtat aaaattacca gaagatgagg ctggatttat agcctttcat atagtgaatg
    16561 ctcagttaag tgaaggtcat actttggcat ctgatattac gaagttaata caagaagtac
    16621 ttagcatagt taggtatcat tttaggatag aatttcatga agaatcagtg ttttattata
    16681 gatttataat gcatttaaag ttttttgcgc aaagattgct tttagatagt gcacatgaag
    16741 gtgagacaga caaagaatta ctaagcatta ttaaatctaa atataataaa gagtttgaat
    16801 gtgtagttaa aattaaaaat ttcattaaaa aacaatataa ctacatatta acagatgatg
    16861 agcttatata tttaacaata catttagcta aagtagttaa agactcaaat atgtaagtga
    16921 aataattttt cagcagtata ttaagaatga ttgaaaggag tgatactaac aaaattatac
    16981 ttatggttgg atggtaacat tgagttggcc aaaccttcaa atttcaaaaa attaatttaa
    17041 acgaaatatg gaggagaaaa atgggaaaat acaaacaatt agcacaagag atagtaaaca
    17101 atgttggtgg aaaagaaaat attagtggat tagtacactg tattacaaga ttacgtttta
    17161 ctttaaaaga tgaaagtata gcaaatgaca atgtattaaa aaacatggaa ggtattgtta
    17221 ctgtaatgaa aagtggagga caatatcaag tagttatagg aaatcatgtt gaagctgtct
    17281 ataaagatgt agttgaaata attggtttag atgattcaag tacaagttca gaaactaaaa
    17341 aatcaggtaa tatactagat aaaggtatag atataatatc aggaattttt cagccagtgt
    17401 taggaataat ggcagcttgt ggtatgttga aaggatttaa tgcattattt gtagcaatgg
    17461 gattatatag tgatgtatct ggtggttata tggtaataaa tgcagcggga gatgcattat
    17521 ttacattcct tcctttgttc ttaggatata cttcagctaa gaagtttgga ttaaaaccaa
    17581 tgttgggatt agcactagga gctgcagtat gttatcctgc tatacaaggt agcagtatat
    17641 cagcaggagc agatgtgatg tacactttat ttaaaggaac aatgtttgca tcaccagtat
    17701 atattgattt ctttggacta ccaattgtat caattgatta tactggaaca gtcgtaccaa
    17761 ttatatttat agtgtatttt gcttctaggt gtgaaaaatt atttaataag tgtgttccaa
    17821 atttagttaa gtttttcttt gtgccaatgc ttacattatt aataacagtg cctgttgcac
    17881 ttatagttat aggccctata gcaacatttg gttctacact tatttcacaa attgtaattt
    17941 caataagaga ttttagtcca ctattggcag gtgctatagt aggatttact tggcaaattc
    18001 ttgttatttt tggtatgcac tggggattta ttcctgtata tataaacaat gttatgactt
    18061 taggatatga taatgttatg atgccattct ttgcctgtac atttgcaagt tcggcagttg
    18121 tgttggcaat atttttcaag actaaagata aaaaactaaa agagatggca ataccaaact
    18181 ttatatctgg tatatttgga gttacagagc cagcaattta tggaatatta ttgcctttaa
    18241 agaaaccatt tataataagt tgtattgcta gtggtattgg aggcgctttc tatggattct
    18301 ttaatcttag aaaatttata actggaggaa tgggaatatt tgaacttcca gcaatgattg
    18361 aaccagatgg gggaatgggt aacttaatag tagctctttc tggtatagct atatcaatgg
    18421 tcgtagcttt tgtattaact atgattttat ataaagatga gaaagtagaa gagtctaaaa
    18481 aagtaagaaa taagggtaaa gaagaaataa aagaagtcaa gtctactaaa ttagaaagag
    18541 aaatagtaac aagtcctata aaaggtgaag tattaaaatt aagtgatata gaggatgcag
    18601 catttgcatc tggtgtacta ggtcaagggg tggcaataat acctagtgat ggaaaagtag
    18661 ttgctccagt agatggggta gttacaacct tatttcctag tctacatgct atagggattc
    18721 tttctgatac aggtgttgaa gtgcttattc atattggatt aaatactatt caattagagg
    18781 gaagaggttt tgaggcatgt ataaaacaag gtgatagaat tacaaaaggt caaactatat
    18841 taaactttga tgttgatgca ataaaagagt tggggtatag tactgtaact ccaattgtta
    18901 ttacaaattc atcacaattt ttagatgtag tagaaacaga aagtaaaaat attgaattag
    18961 aagacaatct aattacagtc ttattttagt aaagattgag gtattagcat aatctagtgc
    19021 tttggttata attttaggag gctttgacta tgagttttaa gaatgatttt ttatggggag
    19081 gagctactgc ggccaatcaa tgtgaaggtg gatacaatga agacgaaaga ggattggcaa
    19141 atgtagatgt atgtccaaca ggaaaagata gaacagcagt tattacaggg aaactaaaaa
    19201 tgtttgattt tgatgatgag cattattatc cagctaaaac tggtattgat atgtatcata
    19261 attataaaga agatataaag ttatttgctg aaatgggatt taaagtatat agaatgagca
    19321 ttgcatggag tcgaatattt ccaaaaggtg atgaagaaac accaaatgaa aagggacttc
    19381 aattttatga aaatatattt aaggagtgta aaaaatataa aatagagcct ttagtcacta
    19441 ttacacactt tgattgtcca atgcatttag ttaaaaagta tggtgcatgg cgtaatcgaa
    19501 aaatgatagg tttctatgag aaattatgca atgttatctt tagaagatat aaaggattag
    19561 tgaagtactg gctaactttc aatgaaataa atatgatttt acatgctcca tttatgggtg
    19621 ctggtatttg ctttgaagaa ggtgaagatg ttgaaaagat aaaatatcaa gctgcccatc
    19681 atgagcttgt agctagtgca attgcaacta aaatagcaca tgaagttgat tcaaataact
    19741 taattggatg tatgtttgca gctggaagtg tatatcctta tagttgtaat cctaatgatg
    19801 tatgggaagc tacaaagtta gatagagaaa attacttctt tgtggatgta caatctaaag
    19861 gtaaatatcc taactatgcg ttaaagtata tggagcaaaa aggtattaca ccagaaatgg
    19921 aacctggtga tattgaatta ttgaataaat atacagtaga ttttatttca ttttcatatt
    19981 acaatagccg ttgtgttaga accgatgaaa atgcagatga tatggctgaa ggaaacatat
    20041 ttacatctgc taaaaatcca tatctttcat acagtcaatg gggatggcca attgacccat
    20101 taggtttgag aattacacta aatcatgtat atgatagata tgaaaagcca ctatttgttg
    20161 tagaaaatgg attaggtgct aaagatatag ctgatgaaaa tggttatgta gaagatgatt
    20221 acagaattga ttatataaga gaacatataa aagctatgca tgatgcagtt tctatagatg
    20281 gggtcgattt attaggttat actacatggg cacctattga tttagtaagt gctggtacag
    20341 gtgaaatgga aaagcgttat ggatttatat atgtagatag agacaatagt ggtaatggaa
    20401 gtctaagaag aatgaaaaag aaaagttttg aatggtataa gaaagtaatt gcttcaaatg
    20461 gtgaagattt atattagaca agaaatctat gttaaataag aaatttgtat taaataagaa
    20521 gagtataaaa attaataata agtataattt ttaacttttt taactataga aaatcaatac
    20581 agaattattt tacattgtat ttatgcaata gtatgattca caaataaaat tttagaaaat
    20641 aaaattgtta agaagtatgg gaagtaagtg caatttactt tgtaataaaa gatgaaaaag
    20701 gcaagcttag gttttaagct tgtctttttt attatgtata aactatgata ctttaaaatt
    20761 ctatgaaaaa tttatttaat agagaaatta aaaatttaat ggtaatcaaa ttctttacca
    20821 atgaatataa aaaatgtcta cgtgttataa tagtttaaac taataaacga aaggtagatg
    20881 aatgaatatg aaataccaac atataataga agttgacaaa gaactttgca ttggatgtgg
    20941 gctttgtaaa aatgattgtc cagtaaacaa tataattata gaaaataaaa agtcagtaat
    21001 taaaaaacaa gattgtctaa tgtgtggaca ttgtgcagca atatgtccca caaaagctat
    21061 taccttgaca ggatttgatg aacctcctat agaacttaca aataagccaa agctagattc
    21121 agatgaatta ctaatggcaa ttaaatctag aagaagtata cgtaaattta aagacaaaga
    21181 agtttcttca gaaattataa agcaaattat tgaggcaggt aggtataccc ctagtgctaa
    21241 aaattcacaa gatgtatcat atattgtttt agacaataaa aagtcaattt atgaaaacga
    21301 agcagtgaaa ttttttagaa aaataaaacc tattgcaaat atagctatta aatattcaaa
    21361 agaagttgag attgacgata actttttctt taagcatgct cctattgcaa taatgataat
    21421 tacaaaagat aagattagtg gttcacttgc agcatcaaat atggcattaa tggcagagtc
    21481 ttatggccta ggtgttttat ttagtggata tttttcagat gtagcaaata attcgccaaa
    21541 attaaaaaag cttttaggtc taaaacgcag taatcatgtt cttacaacat tagtaattgg
    21601 atacccagat gttaaatata gacgtactgc acagaaagaa gtagcaactg ttagatactt
    21661 ataagagagg gtggatttta tggaaaaaga atgtcaacaa agaatgcaag aaataacgtc
    21721 taaatttaaa gaatgtcgca aagctatctc tgctatgggt gatgagacta gacaattgat
    21781 tttaattgct ctcttagaga gtgattttaa tggtatacga gtgggagaaa tcacaaaaaa
    21841 aacacatctt tctcgtcctg ctgtatctca tcatcttaag atattaaaag aagcagagat
    21901 tgttaatttg cgtagagaag gaacaaaaaa ttattattat cttgattcaa aaaaatcaca
    21961 atggaggtca ctaacagagc taataaatct tatatttgtt ggaatacaac atattagtga
    22021 aaatgatata agaaaaggtg attaaattat acttgattta atacaaaaag attgatttag
    22081 gaactaaaaa gaactaaaaa gttcactttg agacactatc ttttgaacta tacccatctt
    22141 aggagacagt taaaatatgt aaaactgtat aaaacaatgg gtatagttca ttttataatg
    22201 gtttaaatat taagaatata ttattctaaa tagtatcttt ctctgttttg agttttattt
    22261 ttatactcaa tagcttcagt ggggcaacca caaatacatg ccatacaatg agtacagtta
    22321 tttttccaaa ctggtttttt gttttttaaa ttaatattat ttaaaggaca tagctctaca
    22381 cattttccac agccaatgca tttatcagta taatgaaatc ctttggcact aataattgtc
    22441 ttataaaaaa ctgtatttat tataccactt ttaattgtac ctcctaagcc actaggagta
    22501 ataatttcaa attcattttc atttcgtata tcagaaataa tcctatatat ttgtttatct
    22561 gcttctttaa tcatttgttt tgatgtttct ttgtctgttg tagaaaataa agctatataa
    22621 ttctctggca ttttaatttc tgccatacct tttaattgcc attccttgta tttacatagt
    22681 ttttgaatat agtttatgga ttttgctgta tctccaccac aagtcattac aaagtaaact
    22741 ctgttgcttc ctagaaattt tgtttctttt ataaaatccg taaccactat tggcaatctc
    22801 catgcataag taggacatac aaatatgaat ggtttatctg ttgatattaa ttcatctgtt
    22861 ttttcctctt ttataagttg atttagtgat atcagttcat catttaattc ttgtgcaatt
    22921 ttacgagcca catatcggct attaccagtt ccgctaaaat ataaaatcat aataatatct
    22981 ccctaactat tttttatttt aactcttaat tttttattta ttatagtaat ttattgatat
    23041 attctattca aatgtatgta aaatttagta gttaagaaaa ttataacaca ttagtaattt
    23101 ttatacttat tggtaaagaa tttgattacc attaaattta gcaacaagac atcaactgga
    23161 cttgtatatg agaagggata ataatttgct tatgaaagat tataaatata aaggttattt
    23221 tgaaggtgta gaaatatttg ataatgtaaa tgaccttaac ttaaatactt taacaacagt
    23281 tagtagattt gtgtttgttt ttttgtttac gataacttaa ggaaatatgt tacctgaaca
    23341 ataaatttat ataataaaag tatataaata tatatatgaa tgttaagatt agttgataga
    23401 atttaaataa taaatccaat attataaaca taggaggtat gaaaaatatg attaatgaga
    23461 atttaaaaag tctaagaaaa atcaatcagt atacacaaga agaattagct gaaaaactta
    23521 atgtttctag acaatcaata gcaaaatggg aaagtggaga atctattcct gatataggta
    23581 gttgcattaa acttgcaaaa ttgtacaatg ttaaactaga tgacttagtt aatcattcgg
    23641 aagaaaaaac aggtattata gtacctccta aaggaaagtt tttctttgga gcagttgtag
    23701 ttggtgaacg tggtcagatt gtcattccaa aagaagcaag agaagtattt aatataaatg
    23761 caggagataa attattggta cttggagatg aggaaagagg attaggaatt gttcatcaac
    23821 gtgatttgat aaattttata ggtcatgtgg gagtagtaca taaagaagaa tgatttaaaa
    23881 aaattataaa aggaggtatg aaatatgagt aaaatagtat tttttagcat tcctgcatat
    23941 ggtcatacta atcctactat tgaagtagtt cgtgaattag tggatagagg aaatgaggta
    24001 ttgtattatt cttttaatga atttaaagat aaaattgaag gtgctggagc aaaatttatt
    24061 tgttgtgata aataccttcc agaattactc cctggagatg agaaaaaaat tggtaaggat
    24121 tttcctggat taattgagat gatagtagat actactattt ctttagatga aaaagtttgt
    24181 agagaactta aagattttaa tccagattgc attgtatcag attcattaag tttttgggga
    24241 aagttatttg cacaaaaact taatattcca tatgtatgct ctacaacaac ttttgcattt
    24301 aataaacata cagcaaagtt gatgaaacaa aactttatag aaattatacg tatgattttt
    24361 ggtgtaagac gcattaataa aaaaataaag cttttacaaa gaaaagggta tgaagtaaaa
    24421 aattttattt ctatagtttc taatgataat gatacggata ccattgtata tacatcaaaa
    24481 gaatttcaac caatggtaga aaccttctct agtaagtatt catttgtagg tccatcagta
    24541 tcaaagttga ttattgaacc taaagagaga aaaagaaagc taatatatat ttcgcttggt
    24601 acaattaata ataaaaatgt tagcttttat aaaaactgta taaatgcatt taaagattct
    24661 gatgttgatg tgattatgtc tgttggtaga agtactgata ttaaaagttt aggaaatata
    24721 ccaaataatt ttgaggttaa aaatagtgtt gaacagattg ctatacttca aaaaactgat
    24781 gtgtttgtta ctcattctgg gatgaatagt gttaatgaaa gtttatatta tggagtacct
    24841 atggtattat ttcctcaaca ttcggagcag agaatggtag ctaagagagt tgttcatttg
    24901 ggagcaggaa ttatgttaaa agaggataaa tcagaaagta ttaaaaaagc agtatttcaa
    24961 gttataaatg acaatgaata taaagaaaat gcaactaaac tatcaaaaag cttttataat
    25021 gcaggaggct caaaaaaagc agcagatgta atattacaga ttatatgtaa tagtagacaa
    25081 gttgacatat aatagatttg ttaaaaatat atatacatac atgtaattta atcaaagtac
    25141 tgacagtggg gtcttttaac ctaccagttg gggcatatat attgatgcta aatatgaggg
    25201 atgttatatt ttaataaaaa agataacatc ctttttttag tgcattatat cgagttttaa
    25261 ataataaatg aaggaggatt aatatgaaaa aatataaagt tatttttata gtattaatta
    25321 taggtattat aagtgtaaca gggtttgttg tatataaaaa ctgcattaaa ccatggaaag
    25381 atgctgaaac atatattgat aagtatatgg taaaacaaga tatttcaaaa gataatataa
    25441 aaagtattac aaaagaaaaa gccaagaagg caagttacga aggaatacta tacaaagtgt
    25501 attacaaaga tgaccctcga tatgagtatc aatattttta tagtgataat tattatgctc
    25561 tatatgtaaa taaagtaatg ttacaaattt atgatatgga aaataataat aaacttttaa
    25621 gaagtaatga ggaattaaaa agtgtaaagt atccaccaat atatcttaaa tataagccag
    25681 ataaatgatt tatgacaaaa tagaataatt tttattaaaa tttatttaaa aatatcacta
    25741 gagcgtttaa at
//
LOCUS       DAYASV010000048        25470 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYASV010000048 DAYASV010000000
VERSION     DAYASV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.048"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            132..1097
                     /locus_tag="ACGI68_001901"
     CDS             132..1097
                     /locus_tag="ACGI68_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFH0138341.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(1295..3406)
                     /locus_tag="ACGI68_001902"
     CDS             complement(1295..3406)
                     /locus_tag="ACGI68_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HFH0138342.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(3708..4415)
                     /locus_tag="ACGI68_001903"
     CDS             complement(3708..4415)
                     /locus_tag="ACGI68_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0138343.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            complement(4614..6527)
                     /locus_tag="ACGI68_001904"
     CDS             complement(4614..6527)
                     /locus_tag="ACGI68_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFH0138344.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(6611..7702)
                     /locus_tag="ACGI68_001905"
     CDS             complement(6611..7702)
                     /locus_tag="ACGI68_001905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0138345.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(7708..9075)
                     /locus_tag="ACGI68_001906"
     CDS             complement(7708..9075)
                     /locus_tag="ACGI68_001906"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HFH0138346.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(9029..9781)
                     /locus_tag="ACGI68_001907"
     CDS             complement(9029..9781)
                     /locus_tag="ACGI68_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0138347.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(9829..10737)
                     /locus_tag="ACGI68_001908"
     CDS             complement(9829..10737)
                     /locus_tag="ACGI68_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0138348.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(10759..11508)
                     /locus_tag="ACGI68_001909"
     CDS             complement(10759..11508)
                     /locus_tag="ACGI68_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0138349.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(11537..12712)
                     /locus_tag="ACGI68_001910"
     CDS             complement(11537..12712)
                     /locus_tag="ACGI68_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFH0138350.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(12727..13566)
                     /locus_tag="ACGI68_001911"
     CDS             complement(12727..13566)
                     /locus_tag="ACGI68_001911"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0138351.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(13569..14726)
                     /locus_tag="ACGI68_001912"
     CDS             complement(13569..14726)
                     /locus_tag="ACGI68_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HFH0138352.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWHVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(14742..15464)
                     /locus_tag="ACGI68_001913"
     CDS             complement(14742..15464)
                     /locus_tag="ACGI68_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0138353.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LICGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(15476..16549)
                     /locus_tag="ACGI68_001914"
     CDS             complement(15476..16549)
                     /locus_tag="ACGI68_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HFH0138354.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(16571..18277)
                     /locus_tag="ACGI68_001915"
     CDS             complement(16571..18277)
                     /locus_tag="ACGI68_001915"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFH0138355.1"
                     /translation="MDYKNNYEVWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(18323..19879)
                     /gene="murJ"
                     /locus_tag="ACGI68_001916"
     CDS             complement(18323..19879)
                     /gene="murJ"
                     /locus_tag="ACGI68_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFH0138356.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(19994..21049)
                     /locus_tag="ACGI68_001917"
     CDS             complement(19994..21049)
                     /locus_tag="ACGI68_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HFH0138357.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(21144..21830)
                     /locus_tag="ACGI68_001918"
     CDS             complement(21144..21830)
                     /locus_tag="ACGI68_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFH0138358.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(22050..24074)
                     /locus_tag="ACGI68_001919"
     CDS             complement(22050..24074)
                     /locus_tag="ACGI68_001919"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFH0138359.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGSGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(24328..>25470)
                     /locus_tag="ACGI68_001920"
     CDS             complement(24328..>25470)
                     /locus_tag="ACGI68_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HFH0138360.1"
                     /translation="ISNNAVDSKLMNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQ
                     LRHAVWHGVTLADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILF
                     GFFLNYLGNPDLDTVRILGVLQRMGLVYFVTSLVYLLLKKLNVGSTATIITFLCISTF
                     IIVGYYILAKPYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCT
                     MGCVLLKENIGEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGI
                     LLSIFYFICDIKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTL
                     VEWTTYELITPWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         7948 a         4636 c         2759 g        10127 t
ORIGIN      
        1 ttttcttata aattatatca ttgtcttttt tttgtaactg aaacaagtat tgaatatatc
       61 tcaatacttt aatataatta agtgtatgct attaaaaaat atacaggaaa aagaagaaag
      121 aaggggtact tttgtcaaaa ttaaagaaat ttgttatact actggctttc ttggtagtaa
      181 tattccctat aagtgtctat ggatattttt attataaatt atctgctatt catgactcga
      241 gcataagtag tgatttattg gataacaatg accataaaaa tgaagatggg ataattaaca
      301 tactacttat gggaacagat gctagaccaa atgaagattc ttcaaggtca gatgctatga
      361 tgatacttac tattgataac aaacacaatg atatcaaact tacatcatta gctcgtgata
      421 gctatgtaga catccctggt catggtaaac aaaaacttac tcatgcttat gcttatggac
      481 aagctgattt attaatacaa acaattgaag agaattttaa tattgatatt caaaattatg
      541 cttgtgttaa tttcgaatca tttatgtata taattgatgc tattggtggc gttgaggtta
      601 ctgttgaaaa aggagaaata cgagaactaa ataaatttat tcctgaaact tataaatgga
      661 ataaaagtga tgataaaggt agcatacaat acattagaaa ctctggaaag cagactctaa
      721 atggatatca ggcactatct tttgcacgta tcagacacaa cgatacagcc tttgctagag
      781 atggcagaca gagacagatt atacaagcta taatcaagaa aacggaaact ttacctgtta
      841 ctaaatatcc aggtttatta gatgcagttc ttccatatgt taaaactaac atgaagccta
      901 atgcaatact ttctttagga gctcaagttt taaaaatggg cgacttaaat attaagcaat
      961 ttgaatttcc tattgatgat gaaattcata gtacaggtgg tatatatgga aaagctggat
     1021 gggttcttag atttgaccca gatactttag acatattaca cgattttata tttaatgaca
     1081 tagagtttaa acaataaaaa attcaacata aagtttatta aaaagtataa gattaattac
     1141 taaaaaagaa ctatatcgca taattatttt caagttgctg tgtacacttg attacaaaat
     1201 gcatatagtt cttttttatt ttctaattta tcatgataat tttcatttta atctacattt
     1261 ataaaatact ctgtatttta aattaacttt tttattactt aactaagttt aagaaagtgt
     1321 ttaatgtgtt ttgatttata ccatctccag cttcgtatac tagagtagtt ttcttaggct
     1381 ctaaagcagt tttctgagca gatgttacac tatttccaag cataactact ggtgatttta
     1441 atttagctgc taaaggacct gatgttaatg catctaccaa ttgattactt ttacttacaa
     1501 acattgatga atattcagca tttggataga atttttgtat tacttttcca tttgtttctt
     1561 ctctattttc tccagctatt ctatttttag atacatcatt tgtaatgacc ttatccagct
     1621 tatttattac tgaatcagat atacttagat taccacctat aaaataagca tcttttactt
     1681 tcaaatctga tatccaattg taaacttcat cacttacatt atcttttgat actaatacta
     1741 ttggttgctt ttcttctcct gcttttgaag ctatagaaag agaatctgct tcacctgtac
     1801 cacttgttat atatagtttt tctactggat tagtttttac taattctttt gctatcatta
     1861 aagatgtact atatctatct gaaccaccaa ctctatttat tgatacattt gacaatgtat
     1921 cttttatctc actctcaact ttatctccaa tagagttatt tcctccaatt aaaataactt
     1981 tacttggatt aagtcttttt aactcattct tagtggatgt aggtatatta tccttattta
     2041 ccaatagtat aggagcatca tttgatgtag caagtggtgt tgatgttata ccatcaacta
     2101 tagagtaccc atttactaat actactgtat ctgaatttgt agcccaacct tttttactta
     2161 ttgctactgc tgtgtcatat ctttcgtcgc ctcttaattt ctccacttta acattagatg
     2221 gtggagtaac tggttcttca ctactagaat ataaactctt taaatccttt tgtatagaag
     2281 tactatttgc tatatcttta gctgagaaat acatactacc ttttatttca ctatactttc
     2341 tatttaatgt tacttgttgt actatttctt ttgctatatt ttgtccacca taactcgatt
     2401 gaccttgctt atatatacct tgtcctatgt ataagtctac atttgttcct tttacttcat
     2461 tagcccacca tgccactaat ttagaataat ctgctgcttt aagaccaatt ggccagtaaa
     2521 gttgtggaac tacatagtca atcaatcctt gttttatcca tgctcttgta tctgcatatg
     2581 tacctacata actttcatta cctgatgtat ctgaacctgt tgggtcacta ctcttatttc
     2641 tccatatacc tgctgggctt acaccaaata caacattagg ctttatactc tttatagacg
     2701 cttttacatc tctaagtaga gtatttacat tttctcttct ccaatcatcc ttattttgtc
     2761 cttttccata tttttggtat gttgcagtat cattgaaact tactcctgga tagaagtaat
     2821 catcaaaatg aactccatct atatcgtaat tttgaactac ttctgctata ctatccacta
     2881 tgtactttct aacttctggt aaacctgggt cgtagtaata cttatttcca tgtttaacta
     2941 cccagcttgg atttttcttt gctggatggt ttgctggcaa tttatctatg ctctcatctg
     3001 ccatagttat tctatatgga ttaaaccatg catgaaattc cattcctctt ttatgtgctt
     3061 cttctattaa aaatggtaat gggtcatatc cagggtcttt accttgtgta ccggtaagat
     3121 attcagacca tgggtttata tttgatttat ataaagcatc tgattttggt cttacttgaa
     3181 ccactgctgt atttattcct acacttttta atttgtccaa taaatctgtg tattcctttt
     3241 tttgcttggc ttcattattc ttagtcttag gccagtctaa attataaact gtagatatcc
     3301 aagcagctct catttcctta tcatttgatg aatcagcaaa tgaacttaca ctacacatag
     3361 taagtgtcat tatcaatgat aatactaata tacttatttt tttcatttca tcccccttaa
     3421 ttttttaaat ttattttttc tacaatatac ttcttattaa gtatatctaa aattcatatt
     3481 atctgtcaaa tattgaataa tatagttaaa atttatattt gatatttgat tataatatga
     3541 aataatttat tataaaaaaa acagcaaaga aatttatctt ctttgctgtt tttataacgt
     3601 tcatcactat tttaatatta ctaaactctt aaatctctat gtaaaacatt aaatgtaatt
     3661 atatatttaa tgttaatatt caatcctatg taaatcaata tctattatta ttgtaaaaca
     3721 cgtgtagcag ttataagtct tcttgcataa ttagttcctg gagccatgct agtaactttt
     3781 actacatctc ctgtttgtgg tgaatgtatg aatttagaat ctcctacata caatccaacg
     3841 tgatttattg aaccacctga accaaagaaa actaaatctc ctggttttaa ttctgctctt
     3901 gatactttct ttcctactgt tgcttgtgat cttgatactc ttggtaaatt aactcctgct
     3961 ccatttttat agacataaga agtgaaccca gaacaatcaa aagaatttgg tccagttgct
     4021 ccccatttat atggcttacc ttgttgctta aatgctaagt ttaaaacatt ttgtaccttt
     4081 gcatctacat ttccaccttg aatagttaaa taatctccat ttacccatcc tgtaacattg
     4141 tcaacacttt ttattttaat ccatccattt gaatgtaatt ctaaaacttc aactttgtct
     4201 cctgtgttta tctttcctac tactgcatca tttgcactag ctccacttct tacatttaaa
     4261 aaatcacact ggtctactgt tgcactcata ggtgaattag ttggctctgc tgcactagcc
     4321 gttattgctc ctgcttctac tgtgacaact cctgctatta acaccatagc tataaatttt
     4381 ttcaatatat gttttttatt tttaatattc atcataatct caaataccta atcctttcga
     4441 aaatttaaca ttttgttcct tcgtttccaa ttcctaatta acattctatc ttacatcaaa
     4501 ataaattgca accttttttg taatcttttt gtaatctttt tgtaatcttt tgtttctttt
     4561 tagtatatat ttgtttcttt ttgctccccc tggttacaaa ttagaaatat attctacttt
     4621 tctctagttg ttgcaatttc tttgatatta gcatcttctg gtttaatata agttggcact
     4681 aattccctca taataccatc tattttttca acctcttcat tgttagtaat ttctcttaaa
     4741 agttttaagt attttgttat tttttctcta tcaacatcta ttggtctacc aataaaaatc
     4801 tttttatgct ctgtatccaa cagaccttct tctgacataa gtagttcttc atacaacttt
     4861 tctcctggtc taagaccaga aaattcaatt tttatatcaa catctggctc aaatccactg
     4921 aatttaatta agttttttgc taaatccaat attcttacag gttctcccat atcaagtaca
     4981 aatatttctc cacctttagc catagcccca gcttgtataa ctaagcctac tgcttctggt
     5041 attgtcataa aatatctaat aatatctgga tgagtaactg tcactggtcc gccatcttca
     5101 atttgcttct taaaaagtgg aattacacta ccattactac caagcacatt tccaaatcta
     5161 acagcaacaa attcagtctg gctttcagca ttcatagtct gtataatcat ttcagcagct
     5221 cttttagtag ctcccattat gttagtaggg tttacagcct tatctgtaga tataaggaca
     5281 aatttcttag ctctatattt tgaagacaag ccagctatgt ttaaagttcc aaatatatta
     5341 ttctttatag cttcgccagg gctcgattcc ataagaggaa catgtttatg tgctgctgca
     5401 tgaaatacca cttctggttt atatttatta aatatttcat ccattctttt ttcttctctt
     5461 attgatgcta ttactgtttt taaatctaat ttatttttat attttctaag caattcttgt
     5521 tgtattgagt aagcattatt ttcataatta tcaactataa taagctcttt aggattaaat
     5581 cctgcaatct gtctgcaaag ctctgaacca attgagcctc ctcctccagt tattaggatt
     5641 acttttccct caatgtaatt acttatctct cttaaatttg tttttatagg ctctctaccc
     5701 aacaagtctt ctatctctac ttctctaatc tgttttatat caacttttcc atctataatt
     5761 tcgtaaattc ctggtattgt ctttatttta cagttagtgt ttttacatat atcaataatc
     5821 tctttttttc tacgtttctc tatgtttgct attgaaaaga ttatctcatc tacattgtac
     5881 ttttctacca catcttttat atctgaagtt gttcctacaa ttttcacatt gtggattatt
     5941 cttccaattt tatttttatc atcatcaatt atagccactg ctactttttt aagttctgga
     6001 tttcttttta gttcctgtat aaccatttct cctgcatctc ctgcaccaat tataaggata
     6061 tttgaaacct ttcctctaga atatgtcttg ttcatgacta ttcttatagc tctataggct
     6121 attcttgcgc cacttgtaaa cattataata aatattgtat ttaatacata aaacattata
     6181 ggataatcaa accctaataa tttatttatt atataaatcg gaattaaata agctaaagtt
     6241 gccacaaaaa tagatataat ttcttcttct cctgcatatc tccataatgt gttgtagcac
     6301 ttaaacaatc taaatgatac tatatgaagt actatatata tacacgctga aattattata
     6361 tcattagtgg taaatgcatt taaattacct gtcaagctta aagacattcg aaacgctact
     6421 agtacgctac aaatatctag aattaatagt gttaaatgtc taaatttata ttttttaaaa
     6481 agcctattaa aaaagctttc tttctctaaa tctatacttt ctcgcataac tagaagccct
     6541 cctttaaaca gcaaaagcta cgcacctaag catagccaac aacatctatc ttttgctgta
     6601 ttttttcatt ttatttttta tatttatcaa atatagaatc ataaattttt tctatatcat
     6661 taaagtttaa attaacctca taattttctc taacaaattg acgccctttc ttacccatat
     6721 taagtctaag ttcatcattt tccattagtt ttattatagc attttttatt tcttctggtt
     6781 gttttggttc tacaattaag ccagtttcat tttcccatac agattctgga tgtcctccta
     6841 catttgtaac tataacaggt acttcacatg cttctgattc tatagctgcc actccaaatc
     6901 cctctcttaa agaaggaaat acagttacat caaaagaatt aaaagtcttg cttacatttt
     6961 ctggagatat tcttcctaaa aactgaacat cattttctat acccaattct ttagctaaat
     7021 ttattaggtt gtccatctga cttccagagc cacctatttt aagaattatt ttcttatctt
     7081 tatattcatc tttaagcatc ttaaatgctt gtattaaata ttctattcca tatttttttt
     7141 ctaaactctt tataattcct attacaaaag cgtcttcttt ttctacttcc ataggcttaa
     7201 acctatccat atctactcca aaaaaggtta catctacatg tttagctgta tatttattag
     7261 cctctttagc catatctcta ctatttgaaa agatatagtc tgcttttcta aagttatgtt
     7321 ttataatttg tttttgtata aaaccattat tagggaaatc ataaatatca gtaccccaaa
     7381 cagaaataat atatggatga tacccaagta gacttcctat aaatccataa ctacttgcat
     7441 actgtgcatg caatatatct ggctttattt catttactag cttctttatc tgctttattg
     7501 aacctaagta tcccgttttt ttagaaatat tttcattttt tagttcttct acattagaac
     7561 caaaattata aatttttatt ccttccatgt gacctccatt taaagagatt acatgaatgt
     7621 catatccttt atttttaaaa aaactacacc attttaaagt atgaggattt gacccatcag
     7681 ctaaataaca aacagtcttc ataatattta ccttcctatc gaataatact caattcctaa
     7741 ctcttccata tctttaattt tataacaatt tcttccatca aggactaaag gatttttcat
     7801 cttttcctta tacttatgta tatcataatt taatacatct ttccattctg tcattataaa
     7861 acatatctct gcatcttcta tcgcatcatc tacagaatca gcataaagta tagagtcacc
     7921 aaatattttc ttagtattat ttacaccaat tgggtcgtat acagtaactc ttgcccctct
     7981 ttcgagcaaa tattttatat ttggtataga tggtgcttct cttatatcat cagtgtctgg
     8041 cttaaaagtc aaacctaaaa cagcaacctt catatcttga aagctctcta ctaacttaga
     8101 tgcctttcta actaatttaa gtttttgact ttcattaact tctattgcag cttttactgt
     8161 ttttaattca taaccattat gttcagataa ccagtgaaga gcttttgtgt cttttggaaa
     8221 gcaagaacca ccatatccta ttcccgcctt taaaaactta cttcctattc tatcatcata
     8281 actcattcct aaagctacat cttctatgtt agctcctgct atttcacaaa gatttgcaat
     8341 atcattcata aatgatattt taagagctaa gaagttattt gatgcgtatt ttatcatctc
     8401 agcactattt ctatttgtaa ctattatagg ttgattaaaa ggcttataga tttcctctaa
     8461 tatttctcta gcccattcac tttctacacc tataacgatt cttgaagcat gtagagtatc
     8521 ttttactgct gttccttgtg ataaaaactc tggattagat gcaagttcta cttttacatc
     8581 attttttagg tttctattta tataatcttc tatctcttca tttgtaccaa ttggaactgt
     8641 agattttatt actactagag tatcttttgt cacagtttca gctatttgct ttgcaactgc
     8701 ctttatataa tctaggttgg ctgaaccatc acttctctca ggtgtaccta cagctattaa
     8761 tattacatct acatcttcat aagctttttt atagtctgta gtgtaatcta ttcttcctct
     8821 gttgtaattt ttaaccataa gttcttctag gccttcttca tatataggtg ctattccaag
     8881 tttcattgat ttaacttttt gttcatctac atcaacacag actacatttt cattgcctac
     8941 ctctgctaga caaacacctg taaccaaacc tacatatccc gtcccagcta tagctatttt
     9001 cattttttta cctccaagta tataaaactt atactctttt tttaatggca ttaaaaacat
     9061 agaaacaaaa tacatataga ctttttggta aacttaaatt ttcaacattt ctataagtat
     9121 tccaagttcg ttttaatgct tttatcttgt tacttgatat agaatttcca actaatctgt
     9181 aattcactaa atcttcttga atcccatatg catatttttc ctttttaagt aactgtaacc
     9241 aggttgcagt atcttgtcct cttcttacca aaggcatgct gaactctcct acaatctcct
     9301 tatcaatgac cactgtagaa catcctatta tagtatttct aagtaatcct tcatagtcaa
     9361 ttttcttagg agctcttgca accttattcg tttttgaccc atcttgtctc atatatctat
     9421 aagatgtaaa agaaaaacct acattttctt ttaacatata ttctatctga atctctaatt
     9481 ttctactatc ccataaatca tcactatcta caaatgcaat aaatcgacct tccgcattct
     9541 taataccaac atttctagat actgcagctc cagaattttt ttcaagcttt atatatttta
     9601 ttctagcatc ttcttttcga aaacttttta taatttcact tgaattgtcc ttagaacaat
     9661 catccactaa taaaagctgc caatttttat aagtttgatt ttggattgac tttatcgtct
     9721 ctgataaaaa ctcttctgaa ttataaacag gagtaattat cgatactaaa ggctcattca
     9781 tatttttact ctccttatat agcaactagc agtactatac caacaagttt acttagatac
     9841 taattttctt tgtccaatat atttgtaata tcctaatggg aataatgcta aatacaacaa
     9901 tttatcagat acttttctta attcaccaaa ataatgacta tcagacaaca atgagtatct
     9961 ggctaaattt atgtaatttt ttatccacat atatggatat ctacttaatg gatatgtatt
    10021 ttgatttatt aaatataaaa actctaatgc tctacctctt aatgcatttt tacttaatat
    10081 attcttagtc aaaccatcat ccaaataatc tcttattatc ataggtttat ttacacatat
    10141 aactttatag tatttagaca tttcattcca tactacactt tctggtaaaa atctcacttc
    10201 ttttctttct ggaaaaggaa attttataag ctcatcagtt acaaatacta tgcttttatc
    10261 tcccttaacg ccatacttaa aatataggtc tgcaaagtga catattttct tatcttcagg
    10321 catttctgtc cctatcaaat ctccacttgg atatacacaa agtccaacaa ctccactaaa
    10381 tccttcttta ttctctaaag aatcccactc atcattagca atttcaagag aatctttaat
    10441 taaaccatca tcactatcta caatgaaaaa tagctctcca tctgcatttt ttacacctag
    10501 atttattgca gtatgttttc ctccattttc tttgtatata tatcttatat ttaatttatt
    10561 ttctgatata aatttttcaa ctaactcttt agtactgtcg ctacttccat catcaacaat
    10621 caaccattca aaatcattga aatcataagt ttgagctttt aaatcattgt acaaattttc
    10681 aagtaaatga gctctgttat acgtcggagt aaatacagta aatttatatt tatacattaa
    10741 ttaaatcttc ctctctgtct aacttttttt gattccgttt attccataat taataaagta
    10801 gtatatagct ttgtataaag gtattttttc tatatttcta tatatattcc aagtccacat
    10861 agcagctttt attttattac cacttaggga attactagat tttctatata atgctagaac
    10921 ttcatttatt ccatgagcaa tatgcccttt tttcaatatc gataaccata aaacataatc
    10981 ttcatgtctt acaccactca ttcttatttc aaaatccaat ttagacttat caatcacaac
    11041 agtaagacat cctaagatat ttccttttaa taatcttcta taatctacat taggtggaac
    11101 ttttattact ttgtttaatt ttttatcatt ttcatccatc agttcatatg aagtaaatga
    11161 aattacatag tcattttcta acataaaatt tacttgtttt tctaacttac tactattcca
    11221 ttggtcatca ctatctaaaa aagctataaa ttgtcctgtt gccttactta gtgctaaatt
    11281 tctagcatta gagacaccct tattagtctc agtctttata cattttattc tactatcctg
    11341 ttgcatataa gatttgacta tattaggact attatctgtt gagcaatcat caataattaa
    11401 catttcccat tcttgataag tttggtttaa tactgatttt atggttgctt caataaattt
    11461 ttcagaatta tacatgggag taattataga tactaaattc ttcttcatat taccttcctc
    11521 atcatttata aaatatttat ttaattttac ttaatacaca atcatatatc ctcttgcagt
    11581 tcttattatc attatatctg aaaaacttat ttctttcatt tttttggtta tccataacct
    11641 caaaattatt ttcaataata tcaataagtt tattcacggc ttcatcatta ttataagcca
    11701 aagacccaaa catttcactt tttaaatcta tatatgaatc aactttttcc atatattcat
    11761 ccaaatcgaa ttggtaaaac aaaataggtc tattcatata caaaaagtct atggctacac
    11821 ttgaataatc agttatattt gcagctgatt ttctgatttc atttgctaga gttacccctt
    11881 tatctaaaaa cacaattcgt tttcctgtta tattttcttt tatatcatct ataaactcat
    11941 gcataagatg atgcatataa atttttacat ttatatcttt ctcatctaat attttattta
    12001 atttgtcatc atttaaaaaa ttcattatat ttttaaaata atctgtatcc gtaaattttc
    12061 ctgaactaaa ttttatccaa tctctccatg taggagtata taatatatct cgagtttgtg
    12121 caacttcatt tgttggtaaa ttgtcatatc ttgcatttcc aattatataa actgagtcat
    12181 ctaacattcc ccaatcattt accataacat ttctttcaaa ttcagaactt gctgtaacta
    12241 tattatagct tttatatagg ttatctataa tatttttgaa ctttttgttc ataggttttc
    12301 tttttttaaa acttatagtc ccatgattta aaaacacttt tatcgttttt ttatgaaaat
    12361 aattaagaac aggtacagca aaattgtatg gtgctatatc tgctgatggt gcatgtgaaa
    12421 atactatagc ttttgaattg taataataca gatagttttc tacacttcct cttattaatt
    12481 ttttgcctgg aattttatca aatactttac tgtctttatt tactacccaa taagtttcta
    12541 catcattatg ttcttttagc atatactcat aaaaaaactt tgaattatca ttgtatatat
    12601 cccctgaatg tccacctata agccagatat ctctaccatt aaacttatgc ttattaaatg
    12661 gatatataaa aaaagccaaa agcatattta ttaaaaattt aaccttgtcc ataaacacca
    12721 tcctcattat aacttgtact gtctaaatcc attaattctt ccttttatag tggcactatg
    12781 ataatctcta ttggaactca atataagacc tcctatattt cttacaagtt ttgataccat
    12841 aacttttgca aacttataat tttttctata gactatttcc tttttgcaaa gcgctccaaa
    12901 gcctcttcca tagttataat ctttttgata cttttcttta gatttagaat gtttttttgc
    12961 aggatggtaa attatatcat ttccaaagta ttttccttta aaacctaaac tcaataagtt
    13021 taaaacataa tctatctctt caccagcacc aaactctcca ccaacaccca atttctcatc
    13081 aaatcttgta tagtctttac cttcaaaatc aataaaaaaa gttatggatg ttatagtatc
    13141 caaaacgttg gaacttgtga tatcacaagt tccatcatac attttcttaa atgcatcaac
    13201 tttatttgaa tccatagttt tgcaactgta tattttataa tctttatttt tattaaagaa
    13261 atttataact ttttctaatg tatcattttc gtagacacaa tcatcatcag ggaaacctat
    13321 aatttgccct acagcattgt ctatgcctac atttctgtta taactcaatc cttttttaga
    13381 gctttttata tactttatat ctaacttatc aatgtattta tcaactattt ctttaacttt
    13441 gctattatca ttttggtcta ctactataag ttcaaagttt ttgtaagttt gattttctaa
    13501 gctgtccata agtaagtata tatcatcata tctatttaat gttggcatta ttaatgatat
    13561 taacattact acctcctact tttcatcttc cacaaatcta tttccctttt atcatttaat
    13621 atcacttctt ttattagtaa tactactgca aaactaaatg aagcttctaa taataaattt
    13681 tctgcaaaag agtatagtaa aaatataaat aaagctacta aatatgcttt tttatctttt
    13741 tctataaagc tttctattcc tttatacatc aaatacataa ataatataca agcaactact
    13801 cctagtacac taattatata tacataagaa ctatctaatg gattagttgc ttttatagca
    13861 ggactatatg aatttccaaa aggttttaga aaattcccct tttcagccaa gtatacatgc
    13921 cagaacttag gtctacttgc taataatcta tttaataaag tgcttttatc aaatactgta
    13981 ccaataatta cactaaataa agtaagtatt atcggtaatg tctttaggag cattggtagt
    14041 ccttttattt tttttatatc tatccatctt attatttcta caaatattaa tcctgctaat
    14101 atagtgaaaa atccagttct actatatgtg gtttgataaa caaaaaatgc agaaccaaat
    14161 aaaattattc tatcccattt attataatct ttatatctta agaatatata tgctgcatat
    14221 atagtaacca tatgataaaa tacactattt ggattaccaa gtccaaaatc cattcttgta
    14281 acaagcatat ctattttttc tatatatcta tagtggactt cctgattaaa tatcaatgaa
    14341 ggaaatagat taaataataa tatagctacc attaaaaaac atgctgatat tattaaataa
    14401 tttttaacta taaatttatc atctatttgc ttagctgata cagctaataa aacgattaat
    14461 aatatagctc cttccctatt taataaataa agtaagacac aaactccaac tagtaccatt
    14521 tctgttatac tttttctctt gtcaaataaa aaaagtagca ttagtattcc tattgaagaa
    14581 taaaataacg ctgatttaat catccctata tccatatttg ggtcaataaa tgatatcata
    14641 tactttctaa gtaatatgat aaacaaaaca aatatataaa gatattttct aatcataata
    14701 tttctatcat catttaaagt cttcatttta ctttctccct tttagcttcg taaagtatta
    14761 cttgcaaata ttttacaaca cttccatatc ttatttacac ctgttaagtg tttgctttct
    14821 aagaaataat ttctcttttt agcccaactt tttccccacc aatgtatccc aaaagtgttt
    14881 tctgttatac attctggtgt aaagtcttct ttaaatccaa aaggataaaa atattctttg
    14941 tcgtaaacac gtattccttc ttcataatta tttgggtcaa tcttcttata attcttttcc
    15001 ataagatgag tcattatctt tggtattgta aacaatgaag aagtaagtac ttctttttca
    15061 taatactcca ataagtcttt taaaaaagga tgtccttttt ctacacctat tatagcacca
    15121 tttatatact ctctatcatc ttcataccca cagattagcc tattattttc taaaagagga
    15181 gttatatcct ttaatatttg catatctgta tccatataaa ctccaccttg ttcatagagt
    15241 acttttattc ttgtataatc agatatgaat gcccaaagtt ttcttttata acattcttcc
    15301 aaaaacttat ttccttttat ttctttctct atatcaaagt ttttttcatt ccactctact
    15361 atctcatatt ctggaagctt ttctttccaa gaatttatac atatattttc tatatttcct
    15421 tttggaccac caaaccatac ataatgtatt tttttaggaa tcaaatgaac acctcctaca
    15481 tatattcttg tctatcttct tcttttaact gctctactat ttttttgaca tcttgagctt
    15541 tatcttttct acaaaccata actgcatcat ttgtagatac tactatcaaa tcactaatac
    15601 ctattgttga tattaatttg tcatctgaat atataattga atttttagta tctatagtaa
    15661 tattttcacc tcttttgata ttaccttcat catcagttgg atatattgca cctagagaat
    15721 cccaagaacc aacatcattc catccaaaat caccaggaac tactataaca tcattacttc
    15781 tttccataat accataatct atagagattg attgtatagt tgggtatatt tcttttattt
    15841 tttccatttc ttcttttgtt cctaagtatt tacttatatc ttctaatttt tcatatactt
    15901 ttggcaagta tctcttaaaa tcttctagta ttttagatac cttccatacg aacattccac
    15961 tattccatac atacttttta gagtttacat attctttagc aatttcatag tttggttttt
    16021 ctacaaactc cactacctca tatgcaacat cttctatagt attttctcta ttaaaattta
    16081 tgtatccata tcctgttgat ggaaatgtag gagttatccc aatagttact agtttatcat
    16141 tattttctgc tatatagata gctttttcca aaatagattt aaattctttt tcatctttta
    16201 tataatggtc agctggatat acacacataa caccatcacc atattttttc attatgttaa
    16261 atgctgcaaa acctatagct gctgctgtat ttctggcaca tggctctggc aatatattac
    16321 tatccaaaca tttatctttt actatatcct ttaaagcttc caattgcttc tcattggtta
    16381 ctataaataa atcatctttt tttgctagag aatctattct gtttatagtt tcgtttatta
    16441 aagcatcttc tccacttaaa tttataagct gtttaggtac ttcttgtcta ctaagtggcc
    16501 agaaacgagt tcctcctcct ccagccatta tgacattgta tactttcatt atttatctcc
    16561 ccttagcttt ttatatagaa tccactacat ttaatatata agatattaaa ttgttcaact
    16621 taaattctac atcttcttga ttgtctgaat ttgctccaaa gtaaaattta attttaggtt
    16681 ctgtacctga aggtcttata gctatccaag agccatcttc aagtaaaaat ttaagtacat
    16741 ctgacttagg taaatcatca acaccatctt tatagtctaa aattttatct acttttacat
    16801 cagccacatt atctatttta acacttctaa agtaaagcat catctctttt atttttttta
    16861 ttccatctat gccttttaaa gttactgact ttaagtcctc tttgtagtat ccaaatttat
    16921 tatatgtatc tatcaaagct tcatacaagt tcataccttt tgaagaatag tatgctgcca
    16981 tttcacatat cataagtgaa gatacaacac catctttatc tctagcatga gttccaatta
    17041 agtaaccata gctttcctca tatcccatta taaaacttct atcattgctt tcttcaaacg
    17101 cttttatttt ttctcctata aatttaaatc ctgttaaagt atttaaacaa tctacattat
    17161 aagcctttgc tatttttgca cctagttctg atgtaactat agtttttatt attgtaggat
    17221 tgtttttagg aagtttattt tcttcaacta aactctctaa tatatatcta actaaaagtg
    17281 aaccgacttg atttccattt aagactacat attcaccact tgaatctttt acaacgatac
    17341 ctactctatc acaatcaggg tctgttccta ttatcaaatc agtgccattg ttctttgcca
    17401 tttcaatagc tatattaaac actgactttt cttcaggatt aggatacttt actgttgaaa
    17461 aattagaatc tggcaattct tgctccttaa ctaccattac attttcaaag ccacattcat
    17521 ttaagacttt tcttactggc ttatttcctg taccatgaat aggtgtatat attattttta
    17581 attttttccc gtattcatct attatatttt gtcttaaaac ttgcttctta acagctttta
    17641 taaactcatc atctacttca ttatctaata tagttattaa atttttagat aaagcttctt
    17701 caaaatctat acttttaata gtactgtaat catcaatctt attaacttct gctattatat
    17761 catttgccat atctatacag atttgaccac catctgagtc atatacttta tatccattgt
    17821 attctttagg attatgactt gctgttatta ctattcccat agcacacttt aaatatctaa
    17881 cagcaaaaga aagttctggt gtaggcttta attcttcaaa aatataagcc tttacaccat
    17941 aagcacttaa agtctttgct acttctatac aaaactccct agacatgtat ctactatcat
    18001 gtgctataac tattcccttt tgcttacctt cttctccttg agttttcatg atataattta
    18061 aaacgcccag tgtcgcacgt ctaacagtat aaatattgat tctattagtt cccgctccta
    18121 ttatacctct taaaccacct gttccaaact ctaaattttt ataaaatcta tcttgtattt
    18181 ctttttcatc atcttttata cttaatagct catttttcgt ttgttcatca aaataaggag
    18241 aatttaacca cacctcataa ttattcttat aatccatatt ttcatccttt ctgactctaa
    18301 tttaaaattt caaggtaatt atctatcttc taagtagttt ttgttttcct tttcttaaaa
    18361 attcaaaagc taaatccatc tcttctacct taaataaaac tatcaataca gtatatacag
    18421 aagctccacc taatactcca gctccaactg atataatttc tttaattgtt cctgaaccca
    18481 taaatgcata tagattatta tatatcaata aagtagctac tgccatcaca ccagatgcta
    18541 ccaaactttt tagaccagtc tttataattt tatcccctcc aaaatatcca tttttctttt
    18601 taagagatat aaataataat ataactgtaa ttatgtttga tgtactagtt gatatagcaa
    18661 tacctgcatg acccaatggt cttatcaata ttaaattaag aactatattt attattaaag
    18721 ctatacttcc atttatcatt ggcgtttttg tatctgaaag agagtaaaat gccctatata
    18781 agacatctct tactccacaa gccaataaac ctagtgaata taatctaagc gctatagatg
    18841 taaggtctgt tgataacgca tcaaatttac ccctttggaa taatattcta acaagaggct
    18901 ctgctaatat aatagctcct atagatatag gaagcaccac caatataata caatttgaag
    18961 attttacaat agtacttata aatccttctt tattatcttt accagacatt tttgctagtt
    19021 taggatatat aactgttatt atagatgtaa caaataatgc cataacaaac tcatttagcc
    19081 tatttgcata gttaagagca gacaactttc catcacccaa agtggttgcc aataatcggt
    19141 caacaaatat atttaactgt ccaacagcta ctcctaccaa cataggcaat actaggttga
    19201 ctaactcttt tatggactca tcctttaaat ttatatatgg cttatacttg taggacttct
    19261 taaacgcaaa tggcagttga aacaatagct gacttgccat agccaatagt gctcctgctg
    19321 gcagtatcca tacattttta cctgcactta gtgctattgc tgctattatt ataatattat
    19381 atgggattcc tataagccca ggaattacaa aactgtcatt cacctgtaaa aacgaactaa
    19441 atatcttact tccactcagg aataaaactc ctgaaatcat tattttagta aataatattg
    19501 ctgcttggaa cttagcaggg tcgtttctaa atcctgctgc aaatactgta acaaattgct
    19561 ctgtaaataa aagaccaata atagctatta ctatagccat tatatagcat atatttaaaa
    19621 cattattagt aaattttaag gcaccttcat ctccgagtct tttctttgtc tcaaagtaca
    19681 taggtatata agttgtagca actgcagagc ctatgactgc tatgattaaa ccaggtatat
    19741 ttaatgttaa tacaaatacg tctgcataca tacctgtccc atagaaattt gcaagtacaa
    19801 gttctctaag gaaacctaat acttttgaaa acatagtggc tgccataatc cacaatgctg
    19861 ctttagctgt ttttgacatc ttctttctcc ttatctaatt taaactctaa aagtagaact
    19921 tttaaaactg cttataaaca ttattctaat taagtaaatt taacctatga aaagcttcct
    19981 tataaatcaa aaactatttc gactcatatc taagtgccct aaatgtaatc aatagtaata
    20041 atagtattaa tactatacaa gataagaatg ttagattaaa tatgctctta aatagactat
    20101 aatcacctac tgtataccca acttcaacta catcatttat attgctatct ttcaagaatt
    20161 tatcttgtgc acgacttaag ttatcactac aaatcagtat tggcatattt tcttttgctg
    20221 atactactcc caaagaaata tattcgttta catcagatct actaccaaag tctaatctag
    20281 cagtatagat tttctttata tctttttcat cataaaactt ctttattaaa tcaacatttg
    20341 tacttgactt atttatttgc tttattcctt ctatattttt taagttagaa tcaatctctg
    20401 caataaactc ttttgaatca cctaatgcat atattttttt aagatttttg ccttttaaga
    20461 aatcaatttg ttcctcaaac ccttcatctt tagcccatat tattggcatt ccacttttag
    20521 cggcataaga atatacagat atggcgttct ctaatcctgt tgttgagcta agtaaaaatg
    20581 cttcatcaaa tcctactgtt ctatacattt ctctagctat ctttaaagat gtatcatatc
    20641 tgtctttccc tcttattctt tctactttca ttcccatttt ttctatatta cgttcagcat
    20701 ctttagatac tgcctttaaa ccacctacta tcgtaacctt ctctggacct agttctttta
    20761 gataatttct tgttactcta ccaagatttt tactcttagt tacaactatt ggagcatttt
    20821 ttgcatatgc taaaggtgtt gcacttatac tatctacaat tgactcttca tttacaagaa
    20881 ttacttcttt agaatcctta aatgctcttt tatttaattc tgtcgaaatc tctggtatac
    20941 tcccttctat caattctctt ttgggatttg catctgcaaa tactcctatc gtactagaaa
    21001 tagagattaa caaaactaac gataatatag tagaaatttt tttaatcata agttctcatt
    21061 ccttttcttt tttgtatttt ttgacaataa aaaagctctt tttaaagagc tttttcattg
    21121 attagtaatc taatatttta aaattatcta gctccgtctc ctgtcaatac tactttaaca
    21181 gttttaaaga gtatcttaaa gtcaagtaat atactcctat tttttatata gtaaatatct
    21241 tctttaagtt tttcttcagg agtgatttcg tatccaccat ttacttgtgc ccaacctgta
    21301 agcccaggtt ttatagcgag cctgttgata aatccaggta tttcctcatt aaattgaaca
    21361 gtaaagttcg gtctctctgg tctaggacct attaagccca tgtccccctt taatatatta
    21421 aacagctgtg gaatctcatc aattctagtt ttcctaataa actttccaac cttagttatt
    21481 ctaggatcat ctttttcagc ccattgtgcg ccaaattttt ctgcgtctgt tctcatagat
    21541 ctcaatttat atatatagaa tcttctcccg cattttccta gtctttcttg cttataagtt
    21601 attggtcctt tgtcctctat tttaataaga attccaaata ttgctattaa aggtaaacct
    21661 atcacaagtc ctattataga taaaactaaa tccataattc tttggtacaa gtcgaataaa
    21721 actgttcctt ttactatgct atagtcaatc tcacttgcaa tttctttatc tgcccctgga
    21781 tagcttttaa ttttaggtat ttcacactca tcattagtat atccagacat agttacctcc
    21841 atattggaat aaaaattttt ttaattctta atttcaactt atttctatct tatttttgac
    21901 ttatttctat attatactca ataatgagca gattatcaat acaagaaaca tcattgtaaa
    21961 caatttgatt ctttacaaaa atatatcatc tcttgttata ttttacatta taacaggaga
    22021 tgatatgttt tgctttaata ttttctattt tattttactg tatcttctat tcctattcca
    22081 atttgagttc ccattgtttt tactttttct gcagtattta acttatccat atcgctctta
    22141 ttgtcaataa atgcacattc aacaagtatt gctggataat tattatttct taacacatat
    22201 aggtaatctt ttccattatc aagtgttctt gtcttgattc ctctattttt catattaaat
    22261 ttttctagta ttctttttaa gatatttgat gctgcagtct tagttgttcc accattttta
    22321 tcttttactt tgtaataaat ttccacacca ttaccagatc cattagacgc attgtaatgt
    22381 atacttgtaa ataaatctgg ctttatagta tttgataatg ctgttctttc tcctaaagcc
    22441 attgttttat ctgtatctct agtcataaca acatttatac cttttgaacg taaatattca
    22501 gtagttgcta atgctgtatt aagagtatat ttcttttctt gtgctccacc atttaatcca
    22561 cttgttgccc ctgaatcaga acccccatga cctgggtcta tcactacagt ttttcctgct
    22621 gctgaacctg aattatctgg ttctgttggt gcattgtgtt ttgataaact agatgctata
    22681 gaatttataa cactatcttt cattccatca ccaatttgat atactgtttc tgctgttttt
    22741 tctgataagt ttgtactatg gtatgcagaa acataagttt ttggagttat aagtattgga
    22801 gcttttaatt tagctgctaa aggtcctgct gttattgaat caactattat gtcagatttt
    22861 gcaactaaca ttgcactcaa ctctttatct ggatagaaag tcttaattac atttgcattt
    22921 gtttcatgtc tatctgctcc agaaactcta ttttttgaag taccattttt tgctatttta
    22981 gatatttggt ctataatttt tgatgataaa ctttgactac cacctatgta ataagcttct
    23041 tctaaacctt gtgaggataa ccaattatat gtaccttgtg gtacagaatc tttattagct
    23101 aatattatag gttgttggtc ttcaccagct tttgatgaaa tattaagagc atcatattca
    23161 ccagcatatc catttgctat gtatatttta ttaacatcat gatacttatc tatttctttt
    23221 gctataagta aagatgtttc atgtctatca actccaccaa tacgagtcac atttacattt
    23281 acagcagatt taatttgaga cactgccttt tgtgaaactg aaccatcatc acctataatt
    23341 attacatcac ttggatttag acgttttaac tctgcttttg tactttctgg tatatcatct
    23401 ttatttgcaa gtaatattgg tgcgtcatat gtagatgcaa gtggagtagc tgtaattcca
    23461 tctgctgcta attctccatt aactattaca accttactag acccatcttt ccaaccttgt
    23521 ttgcttatct caacagcagt ttcaaatcta gtagctccag caagtttttt tactggtgca
    23581 gaagtttttc tcatatattc atttactgct tttgttgaat ttgcatcact tattataaac
    23641 tcttgtgtaa catcataatc cttaagattt aatcccatag actctaagtc ttctttgttt
    23701 aaaactttct gttcatcttt accatttatt tttatgcttt gaagttccca tacatttaag
    23761 tattctgggt ctttgttata gtttattaca ccttcatatg aatctgtctt ttcattgtaa
    23821 tttaaagtag cttcaagtgg catatcataa cacataaaat ttaatgttat tgaatcagca
    23881 tttaattttt ctttaaattt tacacttgca ttagatttac ttcctaacac tacacttttt
    23941 ctttgtaaat ttatattaat atctgaatta gattctgtat ttgcagtagt attttcagcc
    24001 gctaaagcta catttggtgc caccattgct acagacaaca ttcctgttgc caataatttt
    24061 gttgtttttt tcatcatatc ccccctaatt ttttggttct ttaaatcttt aaatcttttt
    24121 taatacatct taagcttgtt ttatattaca taaattcaat attatgtaaa tcttttttca
    24181 ttatagcaca aaaaattaca aattgtaaac tttttgtaat cttttatggc atggttttta
    24241 aattttatcg aggagaaatt ttgattatat ttgatagttt ccgtatattt gaacaaatca
    24301 aagactttat catctaaaaa tattgcacta aattttaata aatatttttt tcttgtacat
    24361 tatagacatt actataaccc agaataggac atacaataaa gaaaatataa gagaatccca
    24421 agttgtacct gcccaaggtg ttataagttc atatgtagtc cactctacca aggtagttgg
    24481 ataatcgact ttgttcgtaa gttttggaac attccaaaaa acatgactca atatttcaag
    24541 gcacatatat gtaaaaatag ggctacttcc aagtgctatt attggggtaa atattttact
    24601 tttatttttg atgtcgcata taaaataaaa tattgacagt aatataccat aactgccagc
    24661 cattaaaagt acaaatgagc tagaccataa tcttttatta aatggaaaat actgattaaa
    24721 gataaaagca cctattagca ggattatgct cataaccagt attttaaaga attttttata
    24781 ttctcctatg ttttctttta aaagtacaca tcccatagtg catcccaaca tacctgaaga
    24841 aattgctact atactagtta aaaaaccatc tggttcaaat tccggcttgt acaaatgtcc
    24901 cttgaagaaa tgtaaatcca ctaactgtgc aagagaacct tctagctcaa atccataagg
    24961 ttttgctaat atgtagtacc caactattat aaatgtagat atacatagaa aagtaattat
    25021 tgtagcagta cttccaacat ttaatttttt tagtagtaaa tatactaaac tagtcacaaa
    25081 atagactaat cccatacgtt gaagtactcc aagtattctt acagtatcta aatcaggatt
    25141 tcctaggtaa tttaagaaaa atccaaataa tatcaataag atacttctct tgaatatact
    25201 aagtattata ctcagtgtgg atttattatt ttttagttta ctatttattg atattggaat
    25261 tgtaacacct aaagatataa caaaaaaagg aaatgcaaaa tctgcaagtg ttacaccatg
    25321 ccatacagca tgtcttaatt gaggatacat tctcatccaa gtcccaggat tattgcagac
    25381 tatcatgagg gctattgata gccctcttat tatatctatt gatttaatcc tactattcat
    25441 aagtttagaa tctacagcat tatttgaaat
//
LOCUS       DAYASV010000049        24355 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYASV010000049 DAYASV010000000
VERSION     DAYASV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.049"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            109..300
                     /locus_tag="ACGI68_001921"
     CDS             109..300
                     /locus_tag="ACGI68_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFH0138361.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="ACGI68_001922"
     CDS             455..1864
                     /locus_tag="ACGI68_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0138362.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTVEDFSSLETALNNIVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQIIDLEDI"
     gene            1995..2684
                     /locus_tag="ACGI68_001923"
     CDS             1995..2684
                     /locus_tag="ACGI68_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFH0138363.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="ACGI68_001924"
     CDS             2687..3622
                     /locus_tag="ACGI68_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0138364.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="ACGI68_001925"
     CDS             3619..4512
                     /locus_tag="ACGI68_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFH0138365.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="ACGI68_001926"
     CDS             4590..5336
                     /locus_tag="ACGI68_001926"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFH0138366.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="ACGI68_001927"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="ACGI68_001927"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFH0138367.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRTLKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEIRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="ACGI68_001928"
     CDS             6193..6897
                     /locus_tag="ACGI68_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFH0138368.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLNTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="ACGI68_001929"
     CDS             complement(7085..7513)
                     /locus_tag="ACGI68_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFH0138369.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="ACGI68_001930"
     CDS             complement(7550..8980)
                     /locus_tag="ACGI68_001930"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFH0138370.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="ACGI68_001931"
     CDS             9112..9696
                     /locus_tag="ACGI68_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HFH0138371.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="ACGI68_001932"
     CDS             9712..10233
                     /locus_tag="ACGI68_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein B"
                     /protein_id="HFH0138372.1"
                     /translation="MEIFSNLLIPIIILYIVIYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="ACGI68_001933"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="ACGI68_001933"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFH0138373.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="ACGI68_001934"
     CDS             12596..13366
                     /locus_tag="ACGI68_001934"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFH0138374.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13704..14135
                     /gene="phnG"
                     /locus_tag="ACGI68_001935"
     CDS             13704..14135
                     /gene="phnG"
                     /locus_tag="ACGI68_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFH0138375.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14150..14725
                     /gene="phnH"
                     /locus_tag="ACGI68_001936"
     CDS             14150..14725
                     /gene="phnH"
                     /locus_tag="ACGI68_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFH0138376.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTNLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14728..15843
                     /locus_tag="ACGI68_001937"
     CDS             14728..15843
                     /locus_tag="ACGI68_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HFH0138377.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRAAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKIKHPIYEDEEITIGDMLVTECEIVSKASQNK
                     TENSKNNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIKKFEEVSLGDI"
     gene            15833..16702
                     /locus_tag="ACGI68_001938"
     CDS             15833..16702
                     /locus_tag="ACGI68_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFH0138378.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16695..17561
                     /locus_tag="ACGI68_001939"
     CDS             16695..17561
                     /locus_tag="ACGI68_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138379.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQREFKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17602..18315
                     /locus_tag="ACGI68_001940"
     CDS             17602..18315
                     /locus_tag="ACGI68_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFH0138380.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18316..19485
                     /locus_tag="ACGI68_001941"
     CDS             18316..19485
                     /locus_tag="ACGI68_001941"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFH0138381.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19489..20325
                     /locus_tag="ACGI68_001942"
     CDS             19489..20325
                     /locus_tag="ACGI68_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFH0138382.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20326..21321
                     /locus_tag="ACGI68_001943"
     CDS             20326..21321
                     /locus_tag="ACGI68_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138383.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21339..23027
                     /locus_tag="ACGI68_001944"
     CDS             21339..23027
                     /locus_tag="ACGI68_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFH0138384.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     EFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGPSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23039..24106
                     /locus_tag="ACGI68_001945"
     CDS             23039..24106
                     /locus_tag="ACGI68_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0138385.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9726 a         2723 c         4345 g         7561 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atgtcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagcgagagg cttcctaaaa cagctcttga
     1081 gcaaggcgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaatatgg gtttaacagg ttatgaacta gaaaatattt taagaaacaa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgtt gaggatttct ctagtttaga aacagcatta aacaatattg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa ataatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag ctatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagtcaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgagtgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggaattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gtgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaac attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttgttt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaaa taagaggaga atttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaacgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaac acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctaactc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagtgatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacagcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agaggcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt taccataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag taatttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atggattaaa agctgcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaagggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactga ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacga
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcaaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgcgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggcacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttccca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagttcat gaggcactct tttttgtatg aaatcaaata tattttcatc atttcaacaa
    13501 tgaaaaagaa gtttatcata ttttgagata gtttgttttt atttatttaa ttcatatttt
    13561 aaaatatatt ttcttaacat tgttaaaaaa gaatttacaa gagattcatt agaattaact
    13621 caactctaat attcacttaa cttaagttgt ttattatatt attaaagaga agtaaacgaa
    13681 aggtagatta aggaggttga attttgtata cagttgatag tactaagata ttgataaata
    13741 tgaacttaga gaaactagaa aagatatact caatgataaa agatacatca agtatttcta
    13801 taattaaaga acctaacttg gctacagtaa tggtaagagc taatgaaagt gtaaaaaaca
    13861 caacatttaa tttaggagag atattagtta ctgaatgtag tgtaaaggta gatgaaagct
    13921 tgggttacgg aatagtttca gaaaacaata ataagaaagc catttattta gcagttatag
    13981 atgcagtttt acatagcaat aacagtaaat ttgatgagtt aaagaattac ataaataaaa
    14041 gtgtatatga agagaactta aggtatgaac aagagattat tgatgaattt agcttgatta
    14101 ataaaacgaa agttcaattc aatgctatgg attaaatagg aggagaatca tggtaaatta
    14161 tacacaagaa atatatagaa agatgctaga tagtgcttca tttcctggga aattaaataa
    14221 tatagataat ataaatatag aaaataatac aaatctattg aatggagcaa ttggtatagc
    14281 aatcacttta ttagaccaag aagttacctt ctatatagaa aattatactc aaaaagatat
    14341 aaaaaatata agagcactta caatgtcaaa tcaaatagat tatagaaaat cagattatat
    14401 atttttagat attaattcag aagtggatat attacagatt aaaataggtt cttttgaata
    14461 tcctgatgaa agtgcaacta tagtacatca agtaaatgat ttatcaacgt actctcaaac
    14521 taagtatata aagcttgatt tgagtggtcc tggtataaaa tctaaaaata gtatatatat
    14581 aaatggaatt agagaagaat ttatagaaag attatctagt ataaataaag attatccaat
    14641 aggaatagat ttgattttag tagataaaaa aggagaaatt gcatttatac caaggtcatc
    14701 aaaattaagt tgggaggtat tatagaaatg gcttatgtag cagttaaagg tggagaagaa
    14761 gccatattac aggctaaaaa tatacttgag ttttataggg taaaaggaga tagcttgcct
    14821 atagatgtaa agcagattga ggaacaaatg tcttatgctg tagacaaagt aatgtctgaa
    14881 ggaagtttat acaacaagga actatcagca ttggctataa aacaagctca aggtgatata
    14941 ttagaagcct ctttctatgt aagagcttta aggtcaacaa tgcctagaat tacatattca
    15001 gaacccataa atacagaaga aatgagaata aaaagaagaa tatcagctac ttttaaagat
    15061 ataccaggag gtcagtatct tggggcaact agagactatg aaaatagaat attgaatgta
    15121 gaattattag aagaagatga aaaaaagaat agacatttta gagatagttt tataaatgaa
    15181 ttagaaacag tagatgaaat ccctgaattt acaaaaatat ctaatatatt tagaaaagaa
    15241 aatttattac aagaagaagt ttgtgaaaac aaattggaag aagttattga tgatattaca
    15301 agaaatagtt taagatttcc agtttctagg gcagcaaagt tacaggcatt aactagaggt
    15361 gaaagcggag tagtatcagg ttttgcttat gctattgtaa gaggctttgg tgatgaacat
    15421 ccatatataa gtgaacttag aactggcaat gtgtttataa aaataaaaca tcccatctat
    15481 gaagatgaag aaataacaat aggtgatatg ttggttactg aatgtgaaat agtttcaaaa
    15541 gcatcgcaaa ataagactga aaattctaaa aataattcgg aagataaaaa taaaatgtct
    15601 ttaggatatg gtctttgtat ggggaacaat gagaataagg ctatatctat ggctatacta
    15661 gacaaatctt tggagccaaa tagtgaaaaa ctagcacaag acgaagaatt tgtattgctt
    15721 catatagatg gaatagattc attaggattt atatctcact ttaagcttcc tcactatgta
    15781 gattttaaag cggatgtaga aagaataaaa aagtttgagg aggtaagttt aggtgatata
    15841 taactatggt tttttagacg aaaacacaaa aaaagaaata agaagaaaga tattaaaagc
    15901 aatatcaata cctggttatc aagttccctt tggttcaaga gagttgccta tagctaaggg
    15961 atggggtact ggaggtttac aattaacact atcattaatt ggtaaaaatg atgtagtaaa
    16021 agtaattgac caaggaagtg atgattctac aaatgcttgt aatataagga gctttattgc
    16081 aagtgtaagt aatgtagaaa ccacaaaaga tacattagaa gctacattga ttcaaacgag
    16141 acatagaata ccagaagaaa aacttaaatc taatcaaata ctaatatttc aagtaccaat
    16201 accagaaacc cttagaatag tagaacctag tgaagtagaa acaagaagaa tgcattcaga
    16261 agaagattat agtaggatgt gggtatatct ttatgaagat atagttagat ttaatgatat
    16321 atctatagca gttgaatatc catgtaaagt aaatgataga tatttaatga atccatcacc
    16381 tatacctaga tttgatataa aaaaattaaa tatgtcagac aatttattct tgtttggtgc
    16441 aggtagagaa aaaagaattt atgctattcc tccatataca aaggtagaac ctttagagtt
    16501 tgaggattat aagtttgaag aggaaaagtt tgaaggaaaa tactgtagtt tatgtaacag
    16561 tacaaataca tttttagatg aagtatatga cagtgacact aatgaaaagt actatagttg
    16621 ctcagataca agttattgtg aaaaagttag attaaaaaat aacagtatag atgtgacaat
    16681 aggaggtact tggaatgagt aaaataaatg actctaaaag attagaaaat atagacaagc
    16741 aagtattaat aggaagaaat atcaatctta tttatggaga tgggtgtgaa aagtgttttg
    16801 aaagcacagg atatgaaagc aataatatat gtaagtattg tggttctata gttgcatgta
    16861 ataatgtaaa tttagaactt tatgaaggag aagtccttgg aattgtaggt gaatctggtt
    16921 caggaaaaag cactttatta aaaatactgt ttttccaaga acatgcaaat agtggagaag
    16981 tatatatatc ccattacaat aaagacacaa atatactaga gctttcagac caaaaaaaga
    17041 gatacataaa gaatcatttt atgggtattg tgtatcaaaa tccccacctt ggtttaaatt
    17101 tagactttag tagtggtggt aatatagcag aaaaattatt gatggcaaat ttatataatg
    17161 ttgaaaaaat aagagataga ggcaaagaac ttttaataaa aactaatata cctgtagaaa
    17221 gaatagacca taaaccaaaa tactttagtg ggggaatgca acaacgagtt caaatagcaa
    17281 aagctctttc aaataaccct ccaatattgc tattagatga ggttacaaca ggtttagatg
    17341 tttctgtgca agcggaagta ttggatttaa taagagaaat ccaaagagaa tttaagattt
    17401 ctatgatagt tgtatcccat gactttgatg ttataaagat gcttgctgat aggacaattg
    17461 taatgaaaaa tggaagtgta attgagtctg gtcttacaga ccaaataatg gaggacccac
    17521 aacacccata tactcagcaa cttataaatt cattgctata aatatcgttt gtaaataagc
    17581 aaaatggggg tttttaataa aatgaaagaa actatacttg aagtaaataa ttttagtaaa
    17641 gactttgagt tacatgcatt gaacaagact ataacggcat gtagtaatat aagttttgaa
    17701 gtttcaaaag gtgagttttt aggaataata ggtaaatctg gagcaggtaa gtctaccata
    17761 ttaaaaagta tctataaaac atatatacca acaactggag aaataatttt taattcagaa
    17821 atatatggta atgtagacct ggctaaaatt ggagaccgag aaatgataaa tcttagaaag
    17881 aaagaaatag gatatgtatc acagttttta aagacacttc cacgaataac tgcaattgaa
    17941 ctggtagtac attctttaat tgagagtggg tttgaaaagg attgttcgta tgatatggca
    18001 aaagatatac ttaaccaatt tgaaataaag gaaaatttat gggatgcgta tccaaataat
    18061 ttttctggtg gagaaaaatt aagattaaat ttagctcaag ctatggttaa aaagcctaga
    18121 cttttgttac ttgatgaacc aactgcatca cttgataatc aatctaaaat atatgtaaaa
    18181 gaaaaaatgt tggagcttaa aagtcaagga actacaatga taggaatttt tcatgatata
    18241 gagtttatgg aaactgttat agataagacc tttactatga caaaagggac tataagtgaa
    18301 agtggggtag cctagatgat aaaactaatt cataatgcaa atattgtatt agaagatatg
    18361 attttagaaa atggtcattt aatcatagag ggagatacaa taaaaaaagt atcaaatgat
    18421 aagattgatt acgcatttga ctttaatgag atagatgaaa taatagatgc aaaaaactta
    18481 tatgtaatac ctggtattat agatattcac agtgatgcca ttgaaaagga aatagagccg
    18541 agaccaagta cattattacc atttaatatg gcattttatg aactagataa aaaactacct
    18601 gcaaatggaa taactacagt ttatcattct atatctttag gggatggagt aggagttaga
    18661 agtattgata actctttaaa gatgattaaa aatattgatt catataaaaa tatagatagt
    18721 aagagcataa atcataaagt tcatttaaga tatgaagtgt tgtattatga aggtttagaa
    18781 aaggttttag aactattaga tgaaaataaa atagactact tatcaataat ggaccattct
    18841 ccaggacaag gtcagtatac aaatccaaca ttttataaag agtatgctac taaagtgtgg
    18901 ggagttacag aaaattatgt agatacatgg ttagatgatt tagtaaatct gcatgataat
    18961 ttagattgga ataaaatagc taatattata ggtatagcca aaactaaaaa tataaatgtt
    19021 gcatcacatg atgatgatac attagaaaaa atggatttca taaaaaatat aggcataaat
    19081 gtatctgaat ttcctataac tcttgaagtt gcaaaacatt caaagaaatt gggtgttcta
    19141 acttgtttag gtgcaccgaa tattgtaaga ggtaaatctc ataataacaa tttaaaagca
    19201 atggatgcta tattggaaga ttgttgtgac attgtgtgtt cggattatct tccatcagct
    19261 atgataaagt ctatgtgtat agttgcagaa agaataaata atttaaataa agcagtcagt
    19321 ttatttacct caaatcctgc taaagcagtt ggtatatatg atgaaagagg tagtataaaa
    19381 gaaaataaaa aagcagattt agttttagta gatattgata gtgaatatcc aaaggttgta
    19441 aatactattg taaatggaaa aacagtttat aaaagggagg tataaagatt gctaacagag
    19501 ttacattgtc atacaagtat atcagataat aattttacta ctgaagaaat aataaaaaaa
    19561 gcagcagaaa agaatataaa tatcttagca ataacaaatc atgacacatt atctggattg
    19621 gatgaagctc ttgtgcttgg agagcattat ggaataacaa taataccagg tatagaaatt
    19681 tcagcctatg attataaaaa tagaaaaaga gttcatattc ttggatataa catagattta
    19741 aacagtcaag aaataaaaaa tttgtgcaat ccaatggtat gtgacagaca taaagcatct
    19801 gttaagatgg taaataaaat tatagattta ggatacaaaa tatctttgga agatgtaaag
    19861 aaatattcat caaagactgg aatatttaaa caacatataa tgaaagctct tatagataaa
    19921 ggatatacag ataatatata ttcaagtcta tataaagaat tgtttcatag aggaaacggc
    19981 aaggtatatg tatctttaaa atatgtggat tacagagatg caataaaagc tataaaaaat
    20041 tctggtggaa tttgtgtgtt agctcacccg ggtcaaatgg ataattttag cgctattgaa
    20101 gagatggtaa aagtaggatt agatggaata gaagtatatc atccatctca caacaaagaa
    20161 ctagaaaaag agtcgctttc ttatgctaag aagtataatc tagtggtcac aggaggctca
    20221 gattaccacg gattttattc tcaacataat tatgaacttg gaagcaagag cttaaataaa
    20281 gaagatttat ttaagtttag gttagcttta ggaggggtat tataaatgtc ttttttacaa
    20341 gtaaaaaatg taggtaaaag ttatggacaa gttaaggtgt taaaagacat atctattgat
    20401 attgaaaaag gagagtttat ttgtttacta ggtccaagtg gttgtggaaa aagtacactg
    20461 cttagaataa ttgcagggct tgaggataaa catggtggaa aaataataat aaatgataag
    20521 gatatgacta attcaccacc tgaaagtaga aattttggta tagtttttca atcatatgca
    20581 ttatttccaa atatgaatgt ttacaaaaac atagcctttg gactagaaaa taaaaacata
    20641 agtaaatcta atattgataa aaaagttaaa gaagttctag aagttgtaga attaagtgga
    20701 tatgagaaaa aatatccatc acaattatca ggtggacaac aacaaagggt tgcattagct
    20761 cgtgcgattg ctttagagcc agacttttta cttcttgatg aacctttatc tgcattagat
    20821 gcaaaagtaa gacttaaact tagggagcag attagaagtt tgcatagaaa actaggcatt
    20881 acaactataa tggtaacgca tgaccaagaa gaagctcttt gtttagctga taagatggtt
    20941 gttatgaata gaggagaaat aatacaagtt ggaacgccaa aggaggtata taaaaatcca
    21001 gaaacacctt ttgtagctga ttttatagga acaataaatt ttatagatga tggtattaat
    21061 aagatagcta ttagaccaga agatataaaa gtagagtcta atagagattc taaagataag
    21121 gatataaaag tgggagaaat tttagatatc gaatttagag gatttaacta tagaattaca
    21181 gttgagtatc gttctaagca gatgaaatta gatgtagttt ccaaagttgc agaacaaatg
    21241 aaactgtgta taggttctaa aataaacttt aagataccta aagaaggaat tgtacaatat
    21301 aaatcagagg gatgtgctta atagctaggg gtgaataaat gataacatca aaaaataaaa
    21361 gaagaataag tgatagcaca atacaaaaat atttcttgat tttttcggta attgtactaa
    21421 tagtatttat attatttcca ttagtatcat tattaaaaaa tgcatttatg gatacaagtg
    21481 gaaattttat aggaatttca aactttagta aatatgttga aaatccaagt cttatagcat
    21541 catttaaaaa ttcaattttt gtatctacag tctcatcttt aatatcacta tgtttagcat
    21601 ttatatattc atacgcaata acaagaacaa acataaagtt taaagaattt tttaaaacta
    21661 tgggaatgtt tccacttttt gcaccaacta tgttatatgg tatttcactt atataccttt
    21721 ttggaaataa gggaatcttt acaaccatgg gtctggaagt accactatat ggtccattag
    21781 gaataattat ttctcagatt ctatttacat ttccacaagc ttttttaata ttatctgtag
    21841 cactttcgat ggctgattac agactttatg aagcagcaga tagcttgggt gctagtgaat
    21901 ttaagaaatt ttgcaaaata acaattcctg gtataaagta ctcattactt agttcattct
    21961 ttgtatcctt tatacttgca tttacagatt ttggagcagc taaggtagtt ggaggaaact
    22021 acaatgtact ggctactgac atatataaac aagtagttgg acaatttaac ataccaatgg
    22081 gagctactgt ttctatggta atgttaattc ctgtactaat agcatttaca ttagataaga
    22141 tggcaagtaa gaaacaaggt atgaccataa ctgcaaagtc tatgccatat aagattaagg
    22201 aaaataagtt tagagataga gtatttttaa tagtatgtag ttttataaca ttgctaattt
    22261 ttattttaat attcgtttca gttataggtt cagttataaa gttatggcca tataatttaa
    22321 gctttacaat ggaacactat acatttaaag gtattttggg aagtggaata aaaacatatt
    22381 taagcagttt gaaaatatca actttaacag caatatttgg aactatattt gtatttttta
    22441 gtgcttatat gatagaaaag gtggataagt ttcctaaatt aagacaaaca gcttaccttt
    22501 tatcaatgat accaatggcc ttacctggct tagttttagg tatatcatat ataacttttt
    22561 ttaactcagc aagtaatcca ttaaattttt tatatggaag tacagcaata ttagttatag
    22621 taaatgtagt tcatttttat tcagtttgtt ttataacctc aaattcatct ttaaaaatgt
    22681 tggataaaga gtatgaatta gttgcaaaat ccataaatat tccattctat aaagtatttt
    22741 ttaatataac agttccaatg tctataacat caatacttga aatagtagta tattattttg
    22801 taaattcaat ggttactgtt tcagcattaa tatttttata tacacctcaa actcagactg
    22861 catcaatatc aatactaaag ttagatgaga taggatatat aggaccatca gctgctatgg
    22921 cagtattagt gcttttaaca aatataatag taagactttt gtacgaattt gtaacaaaga
    22981 aattaaaaaa taacacacaa aaatggcagc aaaaagatgt tgcatagggg aggtaaaaat
    23041 gaaatttaag aagctagttt ctttaggagt attatcaaca cttttattaa gtgtaataac
    23101 aggatgtagt actgattcat caggttcaga ttcaaagtct gatagtaaga caaaagggga
    23161 tttaacagtt tacacagcaa ttgaagaaga tagtatagag ccatatttag ctacatttaa
    23221 agagaaatat ccagatatta aattaaatat tgttagggca tcaacaggag atataacagc
    23281 aagattatta gcagaaaagg ataatcctca agcagatgtt gtatggggag ttgcagccac
    23341 aagcttattg gttgcagatg accaggggat gttagaacca tatgctccaa aaggttctga
    23401 agaaatagag tctgcattta aagatgataa agaagttcct tcttgggttg ggatagatgc
    23461 ttggatgaca ggtataacag taaacactaa agaattaagt gataagaaca tcccagttcc
    23521 tagttcttac gaagatttaa taaagccaga atataaaggt cttatatcta tgcctaatcc
    23581 atcttcttct ggtacagggt acttaacagt atcagccctt atacaaatta tgggggaaga
    23641 aaaagcatgg cagtatatgg ataagttaca tgagaatatt ggtgtataca ctcaatctgg
    23701 ttcagctcca gctacatcag cagcttcagg agaatatcct attggtatat cttttggata
    23761 tagaggtata aagttaaaag aggaaggcta tccagtagaa gtagtatttc caaaagaagg
    23821 ttctggatgg gatatagaag caaatgctct tgtgaaaaaa gataatataa aagaagcatc
    23881 aaaagtattt ttagattggg ctataagtaa agatgcaatg aacgaatact ctaaaaacta
    23941 tgcagttact actatttcta caggaaatcc aataccagaa ggattcccta aaaagccatt
    24001 agagcaaatg atagataatg atttaaaatc agcagcaaaa aatagagaag atatactaaa
    24061 taaatggata tcaaaatatg atggtaagac agaaaaagag agttaagtaa aactatacaa
    24121 atggagtaaa aagaaaaatt ttttttaagt agattttatt attataatat aagttgagaa
    24181 taatataggt caagaaaagt gaatattcta taaaaatgta tagagtaagt ataccattca
    24241 gtttgaatgg tatatttttt atttttagag attaatttag aaatagatat actacagttt
    24301 aaaatagtat attttaattt cattattaaa gtatagatgt tatatataaa gtaaa
//
LOCUS       DAYASV010000050        23976 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYASV010000050 DAYASV010000000
VERSION     DAYASV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.050"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     regulatory      104..205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            278..1621
                     /locus_tag="ACGI68_001946"
     CDS             278..1621
                     /locus_tag="ACGI68_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFH0138386.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1800..3164
                     /locus_tag="ACGI68_001947"
     CDS             1800..3164
                     /locus_tag="ACGI68_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0138387.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3342..5207)
                     /locus_tag="ACGI68_001948"
     CDS             complement(3342..5207)
                     /locus_tag="ACGI68_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HFH0138388.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5443..6660)
                     /locus_tag="ACGI68_001949"
     CDS             complement(5443..6660)
                     /locus_tag="ACGI68_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0138389.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6860..7723)
                     /gene="aroE"
                     /locus_tag="ACGI68_001950"
     CDS             complement(6860..7723)
                     /gene="aroE"
                     /locus_tag="ACGI68_001950"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFH0138390.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7805..9727)
                     /locus_tag="ACGI68_001951"
     CDS             complement(7805..9727)
                     /locus_tag="ACGI68_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0138391.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9791..10639)
                     /locus_tag="ACGI68_001952"
     CDS             complement(9791..10639)
                     /locus_tag="ACGI68_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFH0138392.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11052..11933
                     /locus_tag="ACGI68_001953"
     CDS             11052..11933
                     /locus_tag="ACGI68_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0138393.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12141..12980)
                     /locus_tag="ACGI68_001954"
     CDS             complement(12141..12980)
                     /locus_tag="ACGI68_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFH0138394.1"
                     /translation="MSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEYKYAVSQGD
                     YVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDELPLFLSRL
                     ECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARIVIESRDDV
                     SNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIADILCNPLS
                     RARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIVEPVGPWLE
                     KMR"
     gene            complement(13159..15093)
                     /locus_tag="ACGI68_001955"
     CDS             complement(13159..15093)
                     /locus_tag="ACGI68_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HFH0138395.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15205..16218)
                     /gene="galE"
                     /locus_tag="ACGI68_001956"
     CDS             complement(15205..16218)
                     /gene="galE"
                     /locus_tag="ACGI68_001956"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFH0138396.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16242..17117)
                     /gene="galU"
                     /locus_tag="ACGI68_001957"
     CDS             complement(16242..17117)
                     /gene="galU"
                     /locus_tag="ACGI68_001957"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFH0138397.1"
                     /translation="MQMTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIE
                     EAIESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHY
                     IRQKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGV
                     QKIAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSIL
                     EKQTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENL
                     KDDLMDYMRKKVSEK"
     gene            complement(17309..17629)
                     /locus_tag="ACGI68_001958"
     CDS             complement(17309..17629)
                     /locus_tag="ACGI68_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138398.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17619..19610)
                     /locus_tag="ACGI68_001959"
     CDS             complement(17619..19610)
                     /locus_tag="ACGI68_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138399.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19733..22276)
                     /locus_tag="ACGI68_001960"
     CDS             complement(19733..22276)
                     /locus_tag="ACGI68_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFH0138400.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22554..22888)
                     /gene="rnpB"
                     /locus_tag="ACGI68_001961"
     ncRNA           complement(22554..22888)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGI68_001961"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23020..23697
                     /gene="srtB"
                     /locus_tag="ACGI68_001962"
     CDS             23020..23697
                     /gene="srtB"
                     /locus_tag="ACGI68_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HFH0138401.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23857..23931)
                     /locus_tag="ACGI68_001963"
     tRNA            complement(23857..23931)
                     /locus_tag="ACGI68_001963"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23897..23899),aa:Thr,seq:tgt)
BASE COUNT         7649 a         4149 c         2705 g         9473 t
ORIGIN      
        1 tttttttcaa taaataacct taatttttta taaaatcgac aaaaaaacga ataattttat
       61 tatatttatt gtaaataata caatattacg atataataca acagaaaaac aaatagaaaa
      121 tgttcatata atattagtaa tacggtctaa tagtttctac aaactcacct taaatgggtt
      181 ttctatgaat agaatgtcaa ctctgaatat atgttctatt ctatctatat gaatatatat
      241 ttttttgttt atatcttaaa gttttttgga ggtaaaaatg gtaccaaaca ctaacaatgt
      301 acaaccaggt ggaaataatt catcattaag ctttttagac aaaatgttta aatttagcga
      361 acgtggctct agccttaaaa cagaagtaat aggtggagtt actacattct taacaatggc
      421 atacatagtt ttcgtaaatc cagcaatatt atctgaagct ggaatggata aagccgccct
      481 tataacagtt accatactag ctacagccat tggtacactt atatttgcga ttttaggaaa
      541 tgcaccattt gctctagctc ctggtatggg attaaatgca tttttcacat actctttggt
      601 tataggtgca ggaatcccat ggcaaacagg tcttggggta gtttttctat ctggatgtgt
      661 cttctttaca ctcgcagtaa caggtcttcg aaagacaatt gcagacgcaa ttcctagaga
      721 attggcagta gcatcttcag caggtatagg tcttttcctt gcttttgttg gtttaaaaaa
      781 tatgggtgtt ataatagcta atccagatac tatagtttca cttgcagatt ttacaccaac
      841 agttttaata gccctttttg ctcttatctt aatggcaata ctagaatata aaaaagttaa
      901 aggtggaata ttgataagta tagtagttgc aacaataata tctgttatat ttggatttgt
      961 ggagttgcca acagcaataa tctcaacacc tcctagtata gcaccagttg cttttaaatt
     1021 agatattgta ggtgcattga aattctcatt aattggacct atattctctt tcatgtttat
     1081 agacatgttt gattcattag catttcttat tacttgttgt aaacaaatgg gtcttgaaga
     1141 tgagaatggt gaaataaaag atttaggaag aatgttatat gcagatgttt cttctacttt
     1201 aataggctca ttcttaggaa ctagtactgt tacaacattt ggagaatctg cttctggtat
     1261 tgcagcaggt gcaagaacag gacttgcttc aatagtaaca gcagcattat tcttagctac
     1321 acttatattt acaccattac ttggtatagt tcctacttac gctgcagctc ctgcacttgt
     1381 aatggttggt gtgtttatgt ttgaaagcat aaaacagatt gattttgctg atagcaaaat
     1441 agctgtacct gcttttgtta cagttttatt gatgccttta acttatagta taagtattgg
     1501 tttatgcttt ggatttattt catatataat aatgcatgta gtagctaaag aaactgataa
     1561 aatcagtatt acactttgga ttataggttt acttgcagtt gtaaacttag ttctaagtta
     1621 atatttttaa atagttctag gtcaatattt taaattatat agaataaaat cttatttatt
     1681 ttaaatttat aaatagcaaa atcactttat atgtaaattg attttgctat taactttgta
     1741 tagaaaattt attatcataa tatatagata taaaaatata tagaaaagga gtggtgttta
     1801 tgtcacaatt acttataaaa aatgcatatc taattactat gaatgcagat agagaggtat
     1861 atgaaaatgg aagcatactt attgaaaata atattataaa agaggttggt aagtttgata
     1921 ttaatattgt agataaagat gcagagatat atgatgctaa aggaaaaata ctaatgccag
     1981 ggctagtcaa tactcatgtt cacttatctc aacaattagg acgtggtctt gcagatgatg
     2041 ttgtactttt aacttggctt agagaaagaa tttggccata cgaaagtagc ttcaattatg
     2101 aagattcctt gatttcatct actgcatgtt gtgttgaatt gataaaatct ggtgtcacta
     2161 catttttaga atctggtggt caatatgtag atgctatggt tgaagctgtt gataaaactg
     2221 gacttagagc ctgtttagct aaatcagtta tggatacagg agatggtctt cctgaggcat
     2281 ggcaaaaaac tactgatgag gaaatcaaca ctcaattaga cttattccat aagtttaata
     2341 atacatctaa tgagagaatt aaaatatggt ttgcattaag aactattttt aatacaactg
     2401 aagaattact tgttagaact aaagaactag ctgataaata caatacaggt atacatatgc
     2461 atattgctga aatagctgat gaaatttcat ttgtaaagca aaacaatgga gttggaacag
     2521 ttgaatacct tgataaacta ggtgtattag gaccaaatct tttagctgct catacagttt
     2581 ggcttactca taaggaaatt gatttattta gattacatga tgtaaaagtt tctcatgacc
     2641 caggttcagc tatgaaagtg gttctagggt ttgcatcaat tcctgaaatg ttagaaaaag
     2701 gaatacctgt ttcaattggt actgatggag ctccttcaaa taatagaatg gaccttatga
     2761 gggacatgta tcttacatca ctaatacata aaggaagaac tttaaaccca actgtcgtac
     2821 cagctgaaga agtcttagaa atggctacta taaatggagc aaaatgtgct ttactagaaa
     2881 aagaaattgg tagtcttgaa gtaggaaaaa aagctgatat gattattcta aatccagata
     2941 caattcattg tttaccaatg cataatccaa ttggaaatat agtttattct atgactagtg
     3001 aaaatgtaga ctctactatt tgtgatggta aatggttgat gaaggaaaga aaagtattag
     3061 ttgtagatga atctgaatta ttaaataaag ttaaaaaaca agcttctaaa ataaaagata
     3121 aagcagaagt taaaattcca tctaaattta aaattataaa ataacataaa tttcaattgt
     3181 tttgtaaata tgaatatata aatatattta tatacatcat atatacaaaa caattgtttt
     3241 tttattttat attcattagc attgctcata aaatacacca taattccaga aaaaataacc
     3301 aatcagttag gattacatca tctaagcaat atataaacgt cttagtttat aagttttatt
     3361 ctactcttat ctaaaaccat ctttaaagag ttttcaactg tttcaattac atctttataa
     3421 ctcaagtaca catttgcgta gatacaatct tcatataaat tatattcagt tactctacaa
     3481 tacagcagtt ttcctccatc taataaattg ctcaaaatat agttttcttt tcttgggaca
     3541 tagcctatca catatccagt ttcaccaact attttaatag ctttttcatc atatagatta
     3601 tctttctctc tagttaaata gtatatctta cttttctcca attctttttc aagatattct
     3661 ctatttctaa accttgaacc agcaacagct attgaagtca aatagatatc cttaccatgt
     3721 tcatctactt ttattttttc aatatttaca aattcttttt ttaaattatt actttcatat
     3781 aatactcttc ttaattcatc cttcaatata acattttttt ctttttcata cgcttcttta
     3841 acaattttta attcttcaag agatagtttc tctctgtatc tttttaagat atttatggat
     3901 tcttttctta cttcatttat atttccaaat attcctttta aagatatatt ctttccttca
     3961 ggatatagtg atttacttcc tttaagcaca ataaaaaata acatatcatc ataatattct
     4021 tgagtcaatt tatctttttc tatatctttc atttttccaa ttaaatcatc tattttaaac
     4081 gtctcttcaa aaaaatttaa taattttaat ctagaacttg tcgttccctt tttagatata
     4141 tgccaatata cctcaaaatc tctaatatct ctatttagat atggcaacaa gtcatcaaaa
     4201 gataagttta catcataaat ttctgacatt tttataatat cttctgtttt tccactagca
     4261 tctcttaatg cttcaaaata tatatttttc catttttcac tgagcaagat accatttatt
     4321 tctttttcaa attcaatctt attgcactct atattttcat cttttaagac gtttatagct
     4381 attaggaaaa ttgaatataa actttcaaag ttagtcccat aatttactac agtaggtaaa
     4441 aatcgattca ctaattctaa tttattacct atatatttaa attctacact tagcaagtaa
     4501 ttgcatataa gacgtgctaa attatttatc ttttctttat ctaaatctcg tttttcaaga
     4561 tattcattca aatctacaat tgaaataata tagttcatca ataatcgttc gtactttgta
     4621 tccttataac catcttcaat tagataggaa tttattttag aatctaaatt ttccatatta
     4681 acaatagcaa atacttttat tgaacctgta gcctttttag ataaattaaa taaatatgta
     4741 ttgtaaaatt caagtttttt aatagtatta ctcagataaa atacgtattc tccagattta
     4801 gaaaatgtat ctacaacttc tcttaagttt tctacattta gatatttctc acttaaaact
     4861 aaccctagtt ttacatactc aactttttta ctcttatata ttaattcttt agttaattta
     4921 tataaccatt tctcatcaat aatcatttct tttaccaaag tatttatctt tttcataaaa
     4981 atttcgaaat aacaaattaa agacttagaa ttactaccct ccaataaata aatttcatta
     5041 gattcacttt tatacatatt taaaatttca tctactagat tatttgcaag ctctacttgt
     5101 tctcttaacg gtatactttc atatatacat aggtaattca tttcaaatga ttctttgtat
     5161 ccatcataac cattaaattc ctgcaatcgc ttaaaaacat tctccaccaa attatcctcc
     5221 tttacatgat gtaatttata ttgattatac tacttatttc atcaagttta catttatacg
     5281 caaaaaaata ttactagaaa ttgaaattct agtaatatcc ttattattaa ttatgtaaat
     5341 accctatctt ataactgtaa ataatatcct tattaatttt aattactttt aataaaaatc
     5401 tatcctagat ttagacttct ctatgcatat attatactaa tattagacat cataatcata
     5461 cactttttct ttttcatatc tcatgtttac aaacaatgca agtaaaattc caacaaatgt
     5521 tattgccaca ttaaacaata caacatattt tggtccatta actccccctg attttgtaat
     5581 aatacctgct atatttaaaa taacatagtt tgctatacta gatgcaatca taactataga
     5641 tgttattttt cctttattag ttggaaacat ttcatttgca gttgacgttg ttaactgaag
     5701 aacacctcct gctgctgaat aacctattac aaatccccct aacatacaaa tagttggagt
     5761 ttgaacaaaa tatacaacta atagcataag taaagctatt ataggatata taactaatat
     5821 tcttattggt ttaatatatt ttttaataag taaagaagtt attaatactg cacatattac
     5881 tcccattgag taaaaggatt gaatttttgt agggtcagcc atcccataca atttacccaa
     5941 ttcctgatta caatttaacc ataatacaaa tgtagatgta catgtaaatc caatacaaac
     6001 aagagctata cttgttggtg taaatttcat cttctctgat ttaactgttt tgcctttgtt
     6061 atcaataaca ttattagctg gtggaaatgg taacacagct atcaaaaagg catctactac
     6121 aataagaatc gctgttacta taaaaataac tttaaatgat attgaatttg ctgcaacaaa
     6181 tccaattata aaaggaagca aaaattgtgc taaagctatt gtaaatttag taaacatatt
     6241 tgctattgtt cctaaactag caaatatctc catacaagaa ggagttacac aggtatctaa
     6301 gaatgaattt gctgcccctc caattattgc aaatacatat gctatataaa aatttggtga
     6361 aaatacaatt ccaacaaaga aaattgcatg tagaaagttt cctataagac cacttacttt
     6421 tcttccatac ttatctgaaa taggtcctgc aaatggataa gatataagtc tccctaatcc
     6481 tagtgcagca ataacagcta aaacaatact tacatccaat gttccatctg ataatgtttt
     6541 agccccccac attgatgaaa attctggttt gtattgtcct aaaattgaaa ttccaatacc
     6601 aagtaaaaaa tatgaaaaat acaacgctat ggcagtagga taatattttt tattgttcat
     6661 aatttttctc ccattcttgt taatttatta acatttttgt gtacaataaa ctcatcccca
     6721 taatggattt aaagtatagt cagttaaagc ttcaaattta actggactat gaggcaaaac
     6781 ccactatgac aatttgtaat ttattattaa aaatattgaa atacgtatgt tttttcattg
     6841 tttaagatat tactcttatc tatttaaagc ataattcatt aacttcttct acaggcattt
     6901 ctaatccagt atatagatta aaagcttctg ctccttggta taataacatt cctaatccac
     6961 caatagcctt acatccatta gcttcagcat cttcaatcat ttttgttttc ttaggattat
     7021 aaacgacatc tgttactact aaatcttttc ttaaaacact agtatctttt atatttgttt
     7081 cattgtcata aggcttcata ccaataagag ttgcatttgt caatatatca cttgattgta
     7141 tctcctcata aagtttgttt gtatcttcta aatcatatag attaacaaca cattctggta
     7201 catctttttt tatattctca actgtttgct ctgctctttt ataaaaatca tccttaggat
     7261 taaagataga tatttctctt gctccatcta aagcacattg tacttgtata gcagttgctg
     7321 caccaccagc acccataatt gtaatctttt tacctttaac ttctacccca ttttctttta
     7381 aattacgaac atagcctact ccatctgtta tatgaccaac taactttcca ttgtcattta
     7441 caattgtatt acatgctcca attatacgag ctgctggaga aagttcatcc atatattctg
     7501 taacagcaga tttacatggc atagtaacat tggctccttt tatattaaaa gtttttattg
     7561 ctaaaagtgc ctcttttacc ttatctactg tgatatcaaa ggctaaatat ctataatcta
     7621 aatctaattt cttaaaacta taattataca tcactggtga tttagagtga ccaaccggtg
     7681 tacctattaa tgcaaataat cctgttctac ctgaaatttc cataactata atctcctctc
     7741 aatattatca ttttctttgc aaataaccta ttcagttatt tacaaagaaa aatttattta
     7801 ttatttatat atttaatgct acttcaaaag cttcttttgt tgcatctaat gccctacgag
     7861 cttttactgc atcaccaaca atataagttt cttttacaac ttcttttata acttcactta
     7921 atgggtcgta attacgagaa cccatagcaa gtactactga atcaaaacct tttaaatcat
     7981 gctctgtgcc atctgtaagt aaatagtcga caccatcatc ataaaattta gaaacttttg
     8041 caccagtaat ggtatgtata ttataatttt caaaatcatt cataaggaat ttacggtgtt
     8101 ctgaaattac atctgcacca acttcttctc taagctcaat aacagtaacc tcatgttctt
     8161 gttctccaag gaaggctgcc acttcacatc caaccattcc acctccaaca acaagtactt
     8221 ttttcccaca agatttttta ccatcaagaa ggtctacagc atgaattaat cctgaatcat
     8281 tgattcctgg tataggaagt acaagtggag ttgctccagt agctataata actacatcag
     8341 gtgatttctc ctttataagt tctactgtag cttctgtatt agtacatatt tttacaccat
     8401 attgattaca ttttgaaata taacttctaa ctaagttagt aatatctcct ttccctggag
     8461 gatatgcagc taaacgcatt tgcccaccaa gaacttcaga tttttcaact aaagttacat
     8521 tatgtcctct tttagcacat acccatgctg caagcatacc tccaacacct ccaccaacaa
     8581 caagaacttc tttgctaatt tcagctggct taagctctgc ttcatgtcct aatataggat
     8641 ttgcaagaca ggctataggt ttgccttgga acatatttgg tacacatcct tgtaagcatg
     8701 caatacatgg tatcatttcg tctagttttc catttttagc tttattaggc atttctgggt
     8761 cagccaagct ttgtctaccg aatgctacta aatcacatct tccttgtctg accattaact
     8821 cagcaaactg tggctctgta taacgtccaa ctgttattac tggtatacta acagcttttt
     8881 ttatttcagt aactaattct gcactgaatc cagcatgtaa agtcgtagga gcccacatat
     8941 attcatcatg tatatgtata ctacgtgaaa catgaagtcc atctattcca caatcttcta
     9001 gatatgctgc tattactgaa ctatcgtgta catcaattcc tcctggaaca tcatcagtgc
     9061 tgtttatacg acataatatt gctagagaat ttcctacttt tttacgtata ttttcaatga
     9121 taagacgagg taatctcatt ctattttcaa aacatccacc aaactcatct acacgcttat
     9181 ttgttctttg tgatataaaa ctactaacca gatatccatg agcgcagtga acttcaacag
     9241 catctgcacc agctttttgt gcacgtaatg cagcatctcc ataagattct ataagttcat
     9301 atatttcttc tgttgttatt gctagtggtg tattacgacc atttgatgca ggtattgcag
     9361 aagcagcttt taatggatgt cctgctactt tttcatttcc ttcagaacca gcatgttgta
     9421 attgtataga taccttcgaa cctgcatcat gacaagcatc tattacattt ttaaaactat
     9481 ctattgtgct atcatcatat aaacaaggct tacgaggacc tccttttgct tttttgtcta
     9541 caacactaga ttctatagtt attaatccaa aaccacctaa agcacgctca agataataag
     9601 ccttagaagt ttcacttaaa gtaccatctg tgtttgcaaa attgtttccc atagggggaa
     9661 ctacaaaacg atttggaact gtcattgaac caattcttat aggtgtaaac atagaactaa
     9721 tcttcataag gttttccttc ctttcaagtt ttatattata atactctgta tatttttata
     9781 ttatataagt ttattttgga gatacttcag accatgcttt atccaaaact tttttagttg
     9841 cattaaatac agtttcagca gcagtggata accctttaga tattagagaa tcagatatta
     9901 cttctgcccc tatgactctt ggtgatatat tgtgttcttt tattattttc acaaattcct
     9961 ctgtgtttcc atatccttca cctggagcca aacggtcatg tagtgattca tccctaagtt
    10021 tttcataagg agtttctaaa acatcacaca cttgaattga tactatctta tctgctggaa
    10081 caggtgcaag gtcctctgct gtctgctttg ctctagccca gtgccaagta tctaatataa
    10141 gcattgcatt ttcacgatta catcctttca caacacgcca tgcagtagcc aaatctggta
    10201 caccactata tggcataaac tcaagaccta taataagttc acctgctctg tcacataatt
    10261 ctcccaatgc agttataatt tgttcttctg gtatcatctc taataatcca caatttatat
    10321 gtttaacatt aaataagtga gccatatgat aaacattttg ctctttttct ctttgagctt
    10381 ccgttctatc ttcttctctt ccccattgag ttatatattc tacttctgta actttcatat
    10441 catattcatt aagaattgca atcatttctt catctgtaac acctgcattt tttgcatcaa
    10501 tataattttc agcacgcaat ccaataccat caaacccagc ttcttttgca atacgaactc
    10561 tatcacggaa tgaaacttct gttcccaatg tataagaact aattgtaatt ggaatattaa
    10621 gatttttttg attattcata gcttaaattt gcccccttta ttttctttat aattaatagt
    10681 atttttgatt ttatattttt atttatccta ctagtagtat tattttacct taaaaataaa
    10741 agtatttttt gatttctatt tatctagatt ttataaattt attgtatcac gagactttac
    10801 taataacaaa ttgataattt cctattaatt catagatttt gtttatagat tttatttata
    10861 agataattct tgtatttttc tattaggtcg tataaatata tttatcttat aattcagaca
    10921 aaaaacgata aattttttta aaagctttta ttcttactct ataatcatat ataataaaaa
    10981 tgtaaaaata tcttaaaggt taacttctac aaatatataa ataaaaatac ctatgaaagg
    11041 atgaacaaaa attgaattta tatcacttac gctattttgt tacattggca catttagaac
    11101 actatacaaa agcggcagaa aatttatcta taacacaacc aagcttaagt catgctattt
    11161 ctttactaga aaatgaactt ggggtggctc tatttgaaaa agaaggtaga aatatagtcc
    11221 ttactaagta tggaaaaata tttttaaaag atgtggaaaa atcattagaa attttagatt
    11281 caagtgtaaa atcattaaaa ataactggta ctggtgaagg acaaatagac ttagcttttc
    11341 ttagaacact tggtactgat tttatcccag acatagtgca caaattttta aaatctaatc
    11401 ctgctaaatc aatagatttt aaatttcata ctggtgtaac tactgacatt attcaaggtc
    11461 ttaaagaaag aaaatatgat attgcatttt gttcgaaact tgaaaaagaa aagggaattg
    11521 aatttatacc tgttgccaaa caagatttag tactaattgt accatatagt catccactag
    11581 ctgcaaagga tactatagat ttaaaagaaa ccatacctta tcctcaaata gtatttaatc
    11641 agagaagtgg tcttagatat ataattgatg atatgtttaa aaaaataaat caacagccta
    11701 atatagtcta tgaagtagag gaagaccaag taattgctgg attagttgca aaaaatttcg
    11761 gaattgctgt agtcccaaat atgaatatgc ttagctttac aaaagtaaaa gtaattcaga
    11821 ttattcaccc aagttgggaa agaaactttt acttagcatt tataaaagat agatatttgc
    11881 ctcctgcaat taaaaacttt aaaaactttg tcattaaaaa cgctcaatta taatttatta
    11941 ataaaggggt tatttttaaa tatcctcttt tattcttttt ataaaataaa aaaatctata
    12001 cttttaaaag tatagattcc taactgacag tcggcatccg agtctcagta attatcgtta
    12061 tacaacagca actggattca gttcctgaat aactaggtta cttacattat atcagatttt
    12121 ttaaaatttg tcaattgtat ctaacgcatt ttttcaagcc atggtccaac aggctctaca
    12181 ataggaactt ctttaaatcc ccaatttctt atatcattca cactcgttgg agtatatatt
    12241 ttgtttctga cagatttgaa tattttgttt ttttctccat attgcataca ctttggatta
    12301 gcttctatta accctcttct tgctctgcta agaggattac ataaaatatc tgctatttct
    12361 aaaccttctc tatagtcagc atcttctttt ttggcaatta taaaaccctt aaccttatct
    12421 cttaattctt catctatatc taagtatatt gttccattat taaatacatc aaaaaacgcc
    12481 ttttgtactt ttaagtttga gacatcatcc cttgactcta taactattcg tgcagattct
    12541 acagtttcat tatttacaaa tgaataaaaa ttctgaagaa catgtataaa agccacaaca
    12601 tacggttcct ttgaaggtgc ataatatttc tttaatctag ctttatctac tgttactgat
    12661 attatagtac actctaatct agatagaaat aaaggcaact catcatagaa taacttaatc
    12721 tgtgaatcat caaacttctc aaatgctctt gattttcttg atatatctgt caaatgtatt
    12781 cctatgttac tggttccaaa acaatactct ttaaatgcat ccatttcttt cctcaaagaa
    12841 catacataat cgccttgact tactgcatat ttatattcaa aaattactcc tgtcaatgta
    12901 aatgggtctt catgatttat atcagctctt ccactttcat caataaacat tatatatttt
    12961 ttctttataa tcttactcac cctcttaaca tatgatgata gcatctatat tcattttata
    13021 tgagttttaa aaatattcca agatttttat ataacttaaa cttaatttta tactaaaaaa
    13081 ttgagatgta aaacaatcaa gcttttattg attgaatttc acatctcaat tataattatt
    13141 tttcaatata tatttttact attttgcaag aatatccatt aatttatcca ttactatata
    13201 agatactccg cctcctattt gataaggagt ttcaactttt ttatttgcaa atactctttg
    13261 ttgctctgat gataatcctg aattatccat taaaactatt ggtgaatgta attttacagc
    13321 taatggtcca gctgataaag catctactaa agcatctgat ttactaacta atacagcctg
    13381 taaatcagta tctccataga atttttctat cactttggca ttagtatcta ctctactatc
    13441 tccatatatt ctatttcctg aaatatcttc agtagttata tcattcattt ttgctattac
    13501 tgaatcgtta attgcacttg gtccacctat aaagtatgca ttttctaatt ttctttctgt
    13561 tagccattta taagattctt ctgtaattcc atctttattg actaatatta taggttgttt
    13621 ttcttcccca gctttagaag ctacagataa agcatctgcc tccccatttc cagaaaccat
    13681 atatgcagtt tttatttctt ctcgatttcc aagttcttca gctattcttg tagcagtttg
    13741 atatctatct tgacctgcta ttctctttgt ttctgtgttg aaagtagttt ttatttcttg
    13801 ctctaattta gaacttattg cattttctcc acctatcaag attactgttt taggtgctaa
    13861 taacttcatt tcttcaacag tttcagttgg taaattagct gtattagtaa gtagtattgt
    13921 tgagtcatag ctacttgcta aaggagttgc tgttatacca tcagctattg cattaccatt
    13981 tactaatatt acagtatctg aactatcagc attccagttt ccaactgaac ttatcttttt
    14041 agctgtatca aatctatttg cacctataat tgtatcccat gtaggattta tagttccatc
    14101 ttcattaaaa gtataatctt tcccatctat tttttgagaa ccagttagca tctttccatc
    14161 tttgtctaaa tagtaagact taccatctat ttgttgccat cctgtctgca ttattcctgt
    14221 tttgtcaaag taatacttat taccatctat ttcttgccat cctgttactg ctaaaccatc
    14281 agatcctaga tagtatttct ttccattctt ttcttgccat cctgtttgca ttactccatc
    14341 tgagttaaag taataagttt ttccgtctat tactttccat ccagatactc ttgctccatc
    14401 agttcctaaa taatattttt tgttattaat tgttttccat cctgtttgca ttactcctga
    14461 agcatcaaag taatacattt ttccatttac ttttagccaa ctattctttt ggtatgttcc
    14521 atcagatttt acataatact tcttaccatt ttctgttttc catcctgttt gatttagatt
    14581 tatcttatat tttgccgcta tctttacata gctgtcgtta ctataatcta taattacatc
    14641 tgaacctaca ggaacttggt caaataacca tctaacatct ttattgtgca ttcttataca
    14701 tccaccagaa atatgctttc ctatagaact ctcattatta tttccatgta ttccatatgt
    14761 atctccataa gttcctttta atgctaatcc catccatcta tctcctaatg gatttcttgg
    14821 acttcctcca ggtatattac ctttataata tggtctattt tttatcttgt taactacttt
    14881 tgttttccct gttggtgttt ctgacccctt tttacctgtt gcaactctaa attcttttac
    14941 aagtttatta tttacaaaat atcctagagt attttttctt gaatttacta taatcacatg
    15001 tttactggct gcgcttgatt ctggtacgcc acatgcaaat actaaaacta aagtcagtgc
    15061 tagtactaaa tactttttta gacttttttt cactataact tccccctctt ttagaaacat
    15121 tactacatgt gtctaattat aacataaaat taccaatacc ttatatttac aaatttgtta
    15181 catttataca tataagtatg tattctactc ttcataccca ttaggattca tactttgcca
    15241 tctccatgaa tcttcacaca tctcttcaag ttcatactta gctttccatc caagctcttt
    15301 ttctgccttt gaagaatctg cataacacat ggctacatct ccagcccttc ttcctacaat
    15361 cttatatggt atttcttttc cacttgcttt agaaaatgct tttactaaat caagaacact
    15421 ataccccttt ccagttccta aattataaac aacaagtcct ggattttctt ctaatttttg
    15481 taatgctttt acatgaccag cagctaaatc taatacatgt atatagtctc taactccagt
    15541 tccatcatgt gttggatagt cattaccata aacactcaat tccttcaatt tacctactgc
    15601 tatttttgtt atataaggca ttagattgct cggaactcca tttggttctt cacctattct
    15661 accactttta tgagcaccga ctggattaaa gtatcttaaa agagctatat ccaatgactt
    15721 atctgctttt gatatatcta ctaacatctg ctctatcata agtttagttc ttccatatgg
    15781 attggttaca gaaagtggaa aatcctctaa tataggacaa gtatgtgggt ctccatatac
    15841 agtagctgaa gaactaaata caaacttttt aacaccatat tctctcatta attcaaataa
    15901 atttaaggta cttattagat tattgctgta atattcaatt ggtttttcta ctgattcacc
    15961 aactgctttt aaagctgcaa agtgtataat tgattcaata ttattttctt taaatacaac
    16021 atgcatttca tctttatttc tgatatcaat attataaaat tttactggtt ttttagataa
    16081 ttccttgatt ctatcaacca ctattgagtt gctattactt aagttatcaa ctataacaac
    16141 ttcataacct gattctaaaa gttctattgc tgtatggctt cctatatatc ctgctcctcc
    16201 agctactaaa actgccattg taattacctc ttttctaata tttatttttc acttactttt
    16261 tttctcatat agtccattaa atcatctttt aaattttctc tctttagagc aaagtctatt
    16321 gttgcctcta aaaaaccaat tttatctccc acatcatatc ttctaccttc aaagttataa
    16381 gcataaatag cctcttgttt tccaagagtt tgaagtgcat ctgtcagttg tatctctcca
    16441 cctttaccag gtgtctgttt ctctaatatg ctaaatattg caggagttat tatatatctt
    16501 ccaagtatag ctatatcaga tggagcttct tcaattgcag gtttttcaac catatcttta
    16561 actttatata ctctatcttc gatatgtttt acatccaata taccatattt attagtatct
    16621 tctttagcta tcttttgaac acctaataca gttgttttat actcattata agtatctatt
    16681 aattgtttta agcacggaac atctgaatct actatatcat cacctaataa tacggcaaaa
    16741 ggttcatcac ctataaaact ttttgcacag taaattgcat gtccaagacc ttttggctct
    16801 ttttgtctta tataatgaat atttaccata tttgatatgt ctcttaccat ttctaacatc
    16861 tctgtcttac ctttttgctc tagttctaac tctaattcaa ctgatttatc aaaatgatct
    16921 tctatagatt ttttatttct acctgttatt ataagaattt cttcaattcc cgattctata
    16981 gcttcttcta ttatatactg taaagttggt ttatctacta taggaagcat ttctttgggc
    17041 tgtgattttg ttgctggtaa aaatctagtt cctagccctg ctgctggtat tattgctttt
    17101 ttaactgtca tctgcatttt ttcacccttc ttcgtaaatt ttctattgta ccaaattaat
    17161 ataccatatt tatacttttt cttcaaactc tatattatcc caatatttaa tgctataaga
    17221 taatataagg tagatgaaat ttcatctacc tttagtttta taagttaata ttgtattgtt
    17281 tatattttat ccaaaactta tacaggtact aaatttcatc taagatgcct tttatatcac
    17341 tatctatatc ttcatctaga taagaatcta aatcttctaa gtcaacttct agttcttgat
    17401 atgtcatatc aacactgaca tcctcatcta tatccatttc tgatattaat tctcctaaaa
    17461 tactatttcc atcagaatct aaaccactat ctatatcttc atctatatag ttttcattta
    17521 aaatttcatc taaatcaatc tcgtaataaa cttcattatt tttttcatcc cctgagtcca
    17581 atcctaagaa ctcatagtta aaagtccaaa cattgtattt acttgacata ctttattcct
    17641 cctgtgaaaa acatattttc atctagttta ttggttccat aatcaacaga gttatatatt
    17701 ttttcaatta tgtcttttct tctttctttt gtactattat ttaatgtcaa ttgtttaaat
    17761 acatatgtat ttgttattct ctcgaataat atttctttta tactatcatc atcaatattt
    17821 ctatagtttt tccacaataa ctcaaataac ttaaacattt ccctactagt ttcatctaca
    17881 taataaggat acatattttc tactaaatgg tttaatcttg atatggcttg ttttaaaaat
    17941 acatcaagac ttgcactttc actttctttt aacattctat aattgtattt cttcttatct
    18001 tctcctgaaa ttacaaagat gatattgtct aaactttttt caacttcatt taaataactg
    18061 cttatattat atgataaatc agacaataga tttttaatct gcttaaacac ttgtacatca
    18121 agtttagtat tatacttttc aaacaaatct atttcttgat tttctaatat tcttggcata
    18181 tatgctgaca ataaatctaa attgtttctt tgttcttgta tttgcttata aagtgaattt
    18241 ataacttcta ttcttatttc atcatttaac tctttttcta aaatcttatc aaattcagat
    18301 ttcatatagt aaatatttcc aaagaaatta aatattatca tatcattcat agctttagac
    18361 caattcttat atatcacttc aatagaattt tctttaagta gaggttgctt tgcatatata
    18421 aatacatctt gcataagttt ttgaaccata cttttactta agtttaaaag tactttttga
    18481 ttattaaatt ttatttccgt ttctacaaaa ttagaatttt cgttttcaag ttctttatta
    18541 attacaatgt cttttatatc actatttttt atagatatga tttctttttg aaaagacttt
    18601 agaatagtca agctatcgaa atcttcttga ataaaaaatg gagcatgttt taatgtatat
    18661 cccactattt cattttctat atcttttagg tcatctattt gcataatttc tctattttta
    18721 tcatttatat gtaggttaaa tacattttta ttatcatata atattatatc ttctcctaaa
    18781 tttaacttag aacaattatc tttcgctgac tttacaatag tcttcttaga tttcttattt
    18841 ataaaaatta attggtcttg atattttact aaaagcatgt ttgtgttttt ataatcaaat
    18901 ccatctatat ttcctgatat cttgcagaat actatttcac aatctttagg aagatttcct
    18961 attttttggt tatctttaac tctctcattt aaatcagatg ggactacact atctatattg
    19021 ttattaaata tagatacaga ccttactata atcccctctc tttccatatg aaagtatccc
    19081 ttcaatgaca ctcttttatc ttctaaaatg tatctttcta tatcatcaaa atttatttca
    19141 aatctatttt tcccactgat aaatataaac ttctcattat ctttattaat tccacccata
    19201 taaaacttat tgtcatctaa acttaatata gtcatattat cctttagata gaatataaat
    19261 tctctttgac ttttgcagat taaatcatat tcatccaaag ttattttcat agtcaaattt
    19321 gctgtaacat ttttacttga attttcactt tttatgtaaa aaggcaagtt atttccatag
    19381 acaattattt gagaatcttc taaaactgcc tctaattttt ctttattgtc tgaaaaacta
    19441 tatatatatt ttacacctgt atcctcatta acttcatctg atttgctgat tattatattc
    19501 cctagatttg ttatatgaag ctttttatat tcttttgagt atctacttat gccactaagc
    19561 tcaaacccat ctgaaagctc atatatgatt ttttctccta atttcaacat accttcacca
    19621 cctaaacaga attataagta ttttataaag gtgaataaaa tgtctcaaaa caaataatat
    19681 tttttttaat actttttatt ctgagacata gtttctcctt ttttataatg ctctaagcac
    19741 cttcaactat ttcttcataa agtttatcta aatgtttaac atattcacga tatccagatt
    19801 tgttctttaa ctcacttctt aaatcttcat atttttgttt aagttcttca gcactcaaag
    19861 aaggagctga ttcttttaat tcttctaact tataatcata gtacttatca tacatagcta
    19921 tatatgtatc aatagacttc ttagctagtt cattacattt ttcttgtgct tctccatatt
    19981 gagaataatc acctattttt attttatagt atccattttc ttgaattatt gttgaagaaa
    20041 tacctttagc ttcatattct tttgctgttt cttttgctgt tttttcaact ttaagtgaac
    20101 ctgcataaac agtgtaatat acacctttac catctttata tgcttccatt tcttttgctt
    20161 tcttttcagc ttgtgctttt tcttgagcct ctttttcagc ttttgccttt tcttctgctt
    20221 cttttttctc tttttcagct tgtgcttttt tctcttcttc agttaattta ttgttagctt
    20281 gttgttgagt tgatgacttt ggaatattct ctttatcatt tttcttattg ttgcctactg
    20341 ttataaaata tcctacacca acaataacca ctataactac tgctataata gtttctttta
    20401 atctactctt cttagaaaca acctcttcat catctaaaga atcttcttta tctgatacat
    20461 aatttttcat ttctttcttg ttttctctat atttgtcctt agaagaattt ttagattttt
    20521 tattactatt ttctttcttt ttaataggta ttactactga tttttcattt tctaattttt
    20581 cttcttttat ttcttttgct tttttatctt ctaagttttt actttctttt tcaagcttcg
    20641 attcattatt tgaagatact ttttcatcta agttattatt tattggtttt tctattttta
    20701 cactacgttc ttcttctata tgttgacttg gttcttcttg ttttatttct ttcaattcat
    20761 tttgctctat ttcttctgat ttcacttgat gattttcaac tggtttatct aaaattatat
    20821 ctggagaaat atcatttaaa ttctcttctt cttgttcagc ttcttcattg tcttttttaa
    20881 taaatccaaa taatttagcc ttctttttac tctttttatt ttctggttca attatatctt
    20941 ctttattgct gtcgtactct ttatctgtag tatctttatc ttctacaacg tcacctatat
    21001 cttgattatt actatctact tcttctttag tcccttcttc agcatcatct tctattgaaa
    21061 tactacttct aaagcaaata tctttatctg cattttttat catgtaaatg tattcttcaa
    21121 tatcgatatc tttatttctt ctagttcttt ttgatgcaat ttcatgattt tctaattcac
    21181 tatagttatc ttttattaga ttctgtgctt tattccatgt atcttcacta atttctgttt
    21241 tatttttaat tacatagtcc acaaattcct taaaattttc aggaccaaat tctccaatta
    21301 tagtcagtct cattgcacct tctataaggt ctctatttct tctctttaaa aattctaata
    21361 caactgttgc acaagcaaaa tctttaataa tttctttgcc aaaaccatat gcttcaacag
    21421 aatgaagttc ttctaatcca cataacttct tatattcatt gtgtattgtt tgagatatac
    21481 tttcattatt taagctattt aacttatcta taaacttcga ttctatatca tttgattttg
    21541 attcatgttt tacttttgaa tttttaattt catttagtag catgtcattt tctgaattat
    21601 ctttacctaa ttgcaaatcc tcaatttcag ttgcttttat ttctactttg ttttgttcgt
    21661 tattctcttc cacattatta ggtaccacat ctttattact agctaatatt ttgtttacac
    21721 agaatataat ttctcttttt aattctcttt tcgcatctgc ttgattttta agaaataaat
    21781 ctccacattc tttcttttct aaacttattt ttctaagaga tatattaaga aggtctttat
    21841 ataggtttaa aaccttataa tccttttcta aacgtcttct tctctctgat agttccattt
    21901 tatcataagc taaaaaacta tttaaatcat tggcttttag agagtctaat ccctctttca
    21961 tagccttatt tacatacttt ctacattttg ctgtagttaa ctctaaatct ttttctaatt
    22021 catctatttc aggtatgtac ttatctacta tccatttgcc aataagtggt tctgtatttc
    22081 ttgctaatgt cttataaaac aatatattaa attttaaaag ctctgcatat tttgcttcat
    22141 taaattctac tttttcaaat ttcttttcca aaacaccatc tagcttttca tttattttag
    22201 actcttgttt taaaaaactt gctgtgacat ttaaaacagt gtttcttatt atattatttt
    22261 cactcaaaac cttcactcct taaatcaatt attttattgt aatatttaca ttgtgcaaag
    22321 ttgctaattt gattatgata tctaaataat tatttactaa atttgctaca tcttatatca
    22381 tatcatatta tttacatcat tttacttttt ctttttatat ctatcttata ttataccatt
    22441 tttaatatct gtactaactt atattacaat attcctcaaa attttaaaag tagattagac
    22501 aaatttacaa ataaaaaaac atcagtttaa actgatgttt ttgactatcg aaataattaa
    22561 gcaagtctat aagcggagtt ctgtattaga taatcatctt tctaggccta ttgtcaccaa
    22621 taggctcaag cgacctacct accggagagt acgagcagca ctctttattc ctatcttggt
    22681 cttgctccag gtggggttta catagccaac tagtcgccta gctgctggtg agctcttacc
    22741 tcgcctttcc atccttacca caatagtggc ggtttatttc tgttgcacta tccttaggat
    22801 cgctcccact aggcgttacc tagcaccctg ctctatggag ctccgacttt cctcatgtaa
    22861 gattacatgc gattatctgg cttacttaat atttatttta cttagttatg ttaacacaaa
    22921 ttcttttaca tgtcaaagta atattatact aaaaatttaa atttattcaa aaatagtgta
    22981 atatagatat ataaactaca tttaactcga ggtgatctat tgaaaaaatt atatagaata
    23041 gtaattaaca ttatactagt attagtaata ctttacagtg gatttaatat atactcaaaa
    23101 cttaccaaat acaatcatga tactaaaata tcctcagaac ttcaaaaaaa agaatataaa
    23161 aaagaggatt tatcaaagat taacagtgat tttaaatttt ggctttctgt tgagaataca
    23221 aatataaatt atccagttgt acaatctaaa gataattctt actatcttga caaagatttt
    23281 tataaaaaag attctatttc tggtacactg tttatggatt atagaaataa atctattgat
    23341 gacaaaaata tcataatata tggacataat atgaaaaata aaactatgtt caacaaccta
    23401 aataagttta aagatgctga cttctttaaa aaaaataata aaataaaaat aaccttgaat
    23461 ggaaaagaat ttttatatga tgttttttct gcttatattg tagaatctga ttatgattat
    23521 ctaaaaacaa attttaacaa tgaatctgat tatcaaaatt acataaatga tattacttct
    23581 aaatcattgt ataaatctcc tattaaggta aatagcaatg ataaaatagt tacactttct
    23641 acatgtacct atgaatttga tgatgccaga atggtgattc atggtagatt gatttaaaac
    23701 tcacagacac agtttaaaac aataatttta aactgtgtca tcttttatac ctataatttg
    23761 taaattagac cactaaaagt atttaataca aaataacaaa tacatcaaac aataaaaaac
    23821 aataaaaaaa gactatgcta aaaacatagt ctttactgga gctggtgata ggaatcgaac
    23881 ctacaacctg ctgattacaa gtcagctgct ctaccgttga gccacaccag cacacctatt
    23941 ttattgtaaa ttagcaaaac taatttttaa aagtta
//
LOCUS       DAYASV010000051        23762 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYASV010000051 DAYASV010000000
VERSION     DAYASV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.051"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            141..1487
                     /locus_tag="ACGI68_001964"
     CDS             141..1487
                     /locus_tag="ACGI68_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFH0138402.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNIIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDIIVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEKAYSIFYGDILESCNIVLMNRNIDTLESDSLNEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            1514..2914
                     /locus_tag="ACGI68_001965"
     CDS             1514..2914
                     /locus_tag="ACGI68_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFH0138403.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENRAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            3124..4560
                     /locus_tag="ACGI68_001966"
     CDS             3124..4560
                     /locus_tag="ACGI68_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HFH0138404.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNIGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            4904..5848
                     /locus_tag="ACGI68_001967"
     CDS             4904..5848
                     /locus_tag="ACGI68_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HFH0138405.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            6017..6949
                     /locus_tag="ACGI68_001968"
     CDS             6017..6949
                     /locus_tag="ACGI68_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFH0138406.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            7054..7521
                     /locus_tag="ACGI68_001969"
     CDS             7054..7521
                     /locus_tag="ACGI68_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HFH0138407.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(7765..8637)
                     /locus_tag="ACGI68_001970"
     CDS             complement(7765..8637)
                     /locus_tag="ACGI68_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFH0138408.1"
                     /translation="MKGNSLMLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSIS
                     AFIFSTIITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQ
                     LTFTTLLAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVL
                     LSSLFLSLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLIT
                     GILWSIANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGI
                     ILIMSGVFMLSIIK"
     gene            8785..10749
                     /locus_tag="ACGI68_001971"
     CDS             8785..10749
                     /locus_tag="ACGI68_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138409.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSYTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            10749..11210
                     /locus_tag="ACGI68_001972"
     CDS             10749..11210
                     /locus_tag="ACGI68_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138410.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            11213..11533
                     /locus_tag="ACGI68_001973"
     CDS             11213..11533
                     /locus_tag="ACGI68_001973"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0138411.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            11549..12649
                     /locus_tag="ACGI68_001974"
     CDS             11549..12649
                     /locus_tag="ACGI68_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0138412.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            12769..15369
                     /locus_tag="ACGI68_001975"
     CDS             12769..15369
                     /locus_tag="ACGI68_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFH0138413.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFESSDKEMVSLNKSNDAFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTIFTKGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKKLEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNKMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
     gene            15838..16173
                     /locus_tag="ACGI68_001976"
     CDS             15838..16173
                     /locus_tag="ACGI68_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0138414.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            16310..17587
                     /locus_tag="ACGI68_001977"
     CDS             16310..17587
                     /locus_tag="ACGI68_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein/redoxin"
                     /protein_id="HFH0138415.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            17708..19096
                     /locus_tag="ACGI68_001978"
     CDS             17708..19096
                     /locus_tag="ACGI68_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0138416.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEEL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            19542..20180
                     /locus_tag="ACGI68_001979"
     CDS             19542..20180
                     /locus_tag="ACGI68_001979"
                     /inference="COORDINATES: protein motif:HMM:NF037435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HFH0138417.1"
                     /translation="MKSKEEIEWLLNRDYKIDPVVTFVGDRYQFSIRQRDALKRAVCT
                     DEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISIEVALSGGTLVIGSDGNIRDLAGLRG
                     TYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDAPVSNSGNLKYRILEHAKTWGIETEV
                     ELVKNADVVLEKLDRVVSSDAVIVDKCISYFNVARGIIEEYIKECNIINLNK"
     gene            20298..21167
                     /locus_tag="ACGI68_001980"
     CDS             20298..21167
                     /locus_tag="ACGI68_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0138418.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(21322..22467)
                     /locus_tag="ACGI68_001981"
     CDS             complement(21322..22467)
                     /locus_tag="ACGI68_001981"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0138419.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(22800..23576)
                     /locus_tag="ACGI68_001982"
     CDS             complement(22800..23576)
                     /locus_tag="ACGI68_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFH0138420.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
BASE COUNT         9356 a         2535 c         4029 g         7842 t
ORIGIN      
        1 gatttataat ataactataa tgatgttgta aaatgaaaat tgtcatttca aaattcccaa
       61 aaagagtaaa agtcacttcg gttatagtca aaaaattttt tcagtgaaaa gaatttagat
      121 ttaaaaggga ggaattaatc atggaaattg aaactaaaac tccggattgg attaatgtac
      181 tagaattaaa cagtaaaata gatattactg ggaaatatga tgtaagtaat attatacaag
      241 atattgtctc tgataagtca ctagaaggtt ccattatata ttttccaaaa ggtaattatt
      301 tatttgagaa aggtattaaa attagtcaac aaataacact tcaaggagat tcttattatg
      361 gaggagataa tcaagtatca aatttagata aaaaacaatc aatcatagga acaaccaatt
      421 ttataactcg aggagtttct aatatgagta ttattgcatt gacaggaaca agtcaatgta
      481 tcaaaaatat taatttttat tatgatagtc atgatataga gaaaataccg cctaaaaatg
      541 tatctgctat tacagaatat ggagaaacac aaggactatc tcattttgaa cacctgttta
      601 tttctggatt ttcagggata gggattgaaa ttccatacta ttcaactggc aatgatataa
      661 ttgttagttc atgtggtctt ggtatgaggc ttggagagaa gtcaatgctt tctagtagca
      721 agatttacga atgtaaaaat ggaatggaga ttactactgg tgttagctta aataatgtga
      781 gaatagaaga aataaaagaa ataggtatta ataataaagg atttggtttt aacctgatta
      841 tgaatcttat ggtcgaccaa tgtggatatt gtggatttat gtttgaaaaa atgtgctata
      901 gccagataac tgccagaatt actagatgcg gtcagtatta taaaagtgtt gattataatg
      961 cttatgagaa tatggaagat agaaaagaaa aagcttattc cattttttat ggagatattt
     1021 tagaaagttg caatattgta ttgatgaata gaaatattga taccttagaa agtgattcac
     1081 ttaatgaaca taagatatat gtaatcaagg caaatgaaac aaacaatgta atgctaacat
     1141 gttatgctga agcagataac tttatacaat gtgcaaaggg caacttgctg ttagaaaatg
     1201 gtgaaaatac ttataaattt tataatggtg agatttgttc tgtaggaggt gttcaaattt
     1261 ctgatataga tgaaaaagat ttaatcaaaa taatagagaa cactttgtat atagatgata
     1321 aaggtaagta tttaatggtt cctagattaa aaaagaatac tgtaatatat tcagtactag
     1381 attctacaga agaaataaat aaattacatg gtggaatatg ggaacagatt gatgtaaaag
     1441 tattagctgg ggaatccatg tactattata aaaagataaa agattaaatt atcaaactag
     1501 gaggtagtga ttaatggaag agtctataat aattaaagat ataagtggct ggattaatgt
     1561 acttgattat ggaatttgta atgatgaaac aaaagatgta agttttaagt tgcaagagat
     1621 tatggatgct gtaccagaag attctattat atattttcca aaaggaaaat acttatttga
     1681 aagtggtgtt aagattaaga aacgattaac cttatgtgga gattcttata ttttagcgaa
     1741 tgttcctaaa aatactgctg gagtaacaca gtttactttt aaaggaaaga aaaatcctaa
     1801 aagtgaaaat agagcaatca taacagcaaa aagtgtaaga cactgtatta agaacatttg
     1861 cttttattca gatagttgtg atagagatat gacatctaat catgaacctc caacagatgg
     1921 aaaacctaga tttcatcata atgtaattat aaattataga aatatatctg ctgttatatg
     1981 tgaagataaa cagggagaac caggtcatta tgaaaatcta tcttttagtg gtttttctgg
     2041 aattgcactt gatatgccta ataattctac agcaaatgat attacaattt ttacatccgg
     2101 acttggaatt agtacagggg aaaattcaac aattgctaac agtaaggttt ggggctgtgt
     2161 tgatggaatg ataatttcta ctggaacttt tataaatggt atgagggtag aagaaataaa
     2221 aaaagttgga attaaaaata ttggtagagg tttaaatttt attacaaata tcacaattga
     2281 ccaatgtgga tattgtggag tttggtttga ttctatatcc aatgttcaga tatcaggaaa
     2341 tatcacaagg tgtgggcagt actattatga tgtaagctat gataattatt taatgttgga
     2401 taatagagtt aaagaagcat attctttatt ctatggtaat ttattggagt caagtaatat
     2461 agttttatca aataccaatg aagataattg ggaagatttc aaagaagata agcataaggt
     2521 gtatcttata gaagcactta aaaccaagaa tgtactatta acatgcaata tagatgctac
     2581 tgactggatt gttaagtcag aaaaaggaaa tttaacgtac aataatatgc gacaaactgt
     2641 tatattttac gatggaaaac taagtagtat caatggaatt ggtatatctg atgaagatta
     2701 tggggataaa gtcaagataa aaaaaggaaa tatatatata aatagtaatg ataaggatat
     2761 taagatgcat aaaccagagg taggtgacat tatgtcaaca attattaaag ataaaagtat
     2821 tctttctgct cattatgggg gaacatggga gaaaattggt gaaaaaatag agtttggaat
     2881 aactgtacac tattataaat tgatagagaa ataagtaaat acgtagtatt tgaatagatg
     2941 tattttattg gagagttagg actatagttt taatataaat ggtttggtaa tagcatggat
     3001 tgaataatat aaaaataata taaaaaatta atttgattat gtaaatttct atgaagtagt
     3061 aaaactgtga ctttagtcat aggaggttca aactaagtaa aagggaaagg aatatataat
     3121 attatggaag gaagaactgg gattaaagat ggaagatgga ttaatgtagt agaaagaggt
     3181 attaaaaatg atggttcgta caacataggt cctgctttgc aggatattat tgatgaaata
     3241 gaaacagatt tttgtgtctt atattttcca aaaggtaaat acctatttac aagtggtgtt
     3301 aaaattaata aagaaattgt attgcaaggt gattctaatt catctacagg tactacacaa
     3361 tttataactc gtggagtaga aaatatgagt attattacat tgacagggaa gaaacaatgt
     3421 attaaaaata taaatttcta ttctgatagt tgtgagatac aggtaaataa agaaccaccg
     3481 acaaaaggta atcctaaata tcattatgag atgattatta ataagaatga aaagaatgtt
     3541 gagatgaata atgtatcagc aatcctgtat gataacagta agaatatgat taaaggtcta
     3601 ggtcattatg aaaatattta tataagtggt ttttcgggta caggaattag gataccatat
     3661 tactcaattg taaatgatat tacagtttca tcttgtgggc tgggtattga tacaggaata
     3721 gacacaatta tttctaatag tagaatatct aagtgtcaaa atggaatgag gattagaact
     3781 ggaactagta taaataatgt aaggattgaa aaaatacaga aagttggtct tataagtagt
     3841 caagttaagg ggtatgaggg gtatggaagt tataaaatta ataatattac gattgaccaa
     3901 tgtgggtatt gtggcttttc atttgattca atgaaagata gttatttttc tggtattata
     3961 agaaggtgtg gtcagtatta ttataataca gattatgaca catatttaag tattaaagat
     4021 agagtagaag aagcatattc tttattttat ggaaattatt ttacagaatg taatattgat
     4081 ttagttagtg ataataagga taactgggat gatggcttag aatataaaca taaagtttat
     4141 gtatttaaaa cccttcaaat accaaaatta acattgagat gcaatataga tgcctatgat
     4201 tatatcgtta aatcggtaca tggaggaggt aatttaatgt taaaaaataa ctttaacaat
     4261 tatatattta ttgattcatc taatccagaa gttattaatg gaattaattt taattatcaa
     4321 aagaatggaa acttgttaag gttaaataat ggtaaattaa gttataaaaa tggaaaggaa
     4381 gaaacagcta tattcagacc taattatggt cttattgtag catcaacaga ttcagaatgt
     4441 gaacaaatag aggctaccta tggagaaaaa tgggagaagc ttggttctga agtaattgga
     4501 gaagaaacgg tatattatta taaacatcat gaaccagata aaacaaacca aatacaataa
     4561 aatttgtatt atataatgat ataaagtttt aatatatagt gtttaccttt aatgtaatat
     4621 tggtatattt gtatagtatt aaatttgtta aaaagtaaaa ttgtagggtg cttgaaaata
     4681 gcaccttttt tagtttcatt ataaaattag ttttgaaagt ttaggaataa tgtatattta
     4741 tttttaaaga attataacct tattaaggat attttaaatg ctagaaatga atcagtaaat
     4801 catactggaa ttatcttaaa tgttttgatt aaattaagta tttagagcta taattatatt
     4861 aaaaatatat tagaacataa ttatattact atggaggtta aatatgatag taagtgtgag
     4921 tagaagaact gatatacctg ctttctatag taagtggttt tttaacagac ttgaagaagg
     4981 ttttgtttat gtaattaatc caatgaatcc aaaacaggtt agtaagatag agttaaatcc
     5041 acatactgtt gattgttttg tattttggac aaaggatgta actccaatgt tatatgattt
     5101 aaataaatta agagactata aatattattt tcactatact ataacatcat atggaaaaga
     5161 agtagagaca tgtattttag ataaaagaaa ggtaatagat tcatttaagg aattgtctag
     5221 aaaaataggc aaagaaagag taattttaag atacgacccg attttcttaa gtgaaaagta
     5281 cacagtagat tatcatatca aagcatttgc gagtttatgt aatcagcttg atggatttac
     5341 agaaaaatgt ataataagtt ttatcgattt gtataagaaa acaaaattta atactaaagc
     5401 attacatata aagccaatag aaattacaga aatagaaaca ttatcaaaag agatttctaa
     5461 gattgctcat aagcatggga taatacttga aatgtgttct gaagaatata atttaagtaa
     5521 atttgggatt ggaaaaagta agtgtattga tgatagatta atatcaaaaa ttataggatg
     5581 tgaagtagag gtaaaaaaag atagtacaca aagagatatt tgtggatgtg ttaaaagtgt
     5641 cgatatagga caatacaata cttgcagaca ttattgtctg tattgttatg ctaactttaa
     5701 ttatgcaaaa gtagaagaaa actgtaggct ttataaggaa gataataaat tactcataga
     5761 tactttaaga gatgatgcaa aaataagcat tagagatatg aagactatta aagtagataa
     5821 gaataaacaa attagtatat ttgattaaaa taaatatact aattacctta atatattaag
     5881 aatatagata cttttgataa caaaattaaa cttaatagat attttctata taagaaagat
     5941 ggtcataata tgtcaatttg accatctttt tgatataatt aaaacacaaa taatttagta
     6001 acaaaggagg cagaacttga atttattaaa aaaatatcct atacctattg caagtttgat
     6061 tttatcaatt tttactttag gtaacttatt acaatcttat agtgaatcat tacgtaatgt
     6121 aattggattt ataggattta ttttgtatgc aatttatata gtcaaaataa tattacttag
     6181 aaagggtgtt aaagagcaat tagagaaccc tttaatatca agtacatttc caactataac
     6241 aatggcaaca atgatatttg ctacatatat aaaaccatta tctttagaat taagtattat
     6301 gatttggagc ataggaattg tgggtcatct tgtgttaata gtcttattta gtaagaagtt
     6361 cttagttaaa ttctcaataa aaacggtatt tccatcttgg tatatagtat atgttgggat
     6421 agttgcagct agtgttacgg caccagcatt taatcaactt ttgattggca aaattgcctt
     6481 ttggatagga tttgtatctt atatagttct tataccagta attttaaaga gagtatggat
     6541 aatcaaggaa atgcctgaac catccttacc aagtatagct atattagctg caccagggtc
     6601 actattattg tctggatata ttaattcttt ttcagataaa aataatgtag tattatactt
     6661 gttattcata ttatcaattg tattttatat tatagttttg ggatatttac cgaaattatt
     6721 aaggttgcct ttttatccga gttttgctgc atttacattc cctatggcaa taagtgcaat
     6781 gggtatgaaa ctaatgaatg gttatttaac taaatcaggt cattttataa gttggatttc
     6841 atatgcttct aagttagaag agataattgc tacagtgatt attttatatg ttttagtttt
     6901 gtttattata tatcttagta aatctgaaac tacatctaaa gctaaataag aattattaaa
     6961 tatgtgtatt taataggaat attgatataa atatatgaag ataagataaa ccatagaaac
     7021 ttaatttagc ttaattttat tggaggaaaa caaatgaaaa caaaagaaga atatttaaaa
     7081 gaagttagaa acatatcaga agaatacttt agaaaaggag agtttttttg ctctgaagca
     7141 gttctccaga caataaatga tgctttaggc caacctttaa gtccagagat aactaagtta
     7201 gcatcaggat ttccgatagg acttggaaag gcacaatgtc tttgtggagc aatatctggg
     7261 ggagagatgg ctttaggtat agtatatgga agagttcatg gtgaagctat gaatccaaag
     7321 atgtttgagc atgctaaagg tttgcatgat tatattaaaa aagagtatgg tgcaacatgt
     7381 tgtagagcta ttacaaagaa atgggatggt gataacttta tgtctcccga aagaaaagag
     7441 cactgtatta aaataacagg tcaagttaca gagtgggttg cagataagct tatagaagat
     7501 gaaaaactta atatcaaata gtgtaatatt agttggtgaa tttataaagt taaatttact
     7561 tatagtataa gatacttgat attctcgtat tgatagaaaa atttatagca tatgtattta
     7621 atgaaatata cttatattta gagtgtggtt taatttcata aatacatatg ctattatttt
     7681 aagtatataa gcaaatttat ttaaataaat agaaacagtt aatttatatg tacaaaacta
     7741 tatatgatta gtgtaaataa atacctattt tataatactc aacataaaga caccagacat
     7801 gattagtatt ataccaataa aaatagcaag ccattctttt ttgttcttct tttctttaaa
     7861 aaatataatc cctcctaaag tggctactat cacacaactc tgagagatag aaaaacttat
     7921 tgtaactccc aatgtattag tcgttatgaa catacctaaa tttgctattg accacaatat
     7981 accagtaatt aaattgaata aaacattttc cctatataca atctctttag aaaaatactt
     8041 gacaataata gatgaagtta tcaacatacc aacagattga ggtaatataa ttctatatcc
     8101 ttcaattaaa aatatttgat tagttacaac atataatgat aaaaatagag atgataataa
     8161 tacaactcca acacatttta acatattttt atctgtagat tttttagttt gataatttgt
     8221 gagaagtata ccaagtatta tacataatat tgataaagaa ccaatcagta acattttaga
     8281 actggtccat tcattaaaca agattacagc taataatgtc gtaaaagtta attgtgaacc
     8341 atttgaaatt ggcattactt tagatacact agcaatttga attgctttaa actgcattaa
     8401 ttgtcccaaa ctccagaata tgccagaaat catacttata caaaatgaaa aaaatgtaat
     8461 ttcaggatgt ataaccagag ttattatagt ggaaaatata aacgcactta tggatgtacc
     8521 taaaagctgt tcatgaggag aacttttctt tagatttgca actatgggca tcaatcccca
     8581 tcccattaca ggaagtaaag caataattat tgtcagcatt aaactatttc ctttcatatt
     8641 aaatttagac agcttttaat ttatcatcta aaattaaagt gttcaataca taaaaattac
     8701 cgtatttaat caggaaatat tagtagtttt ttagaaaaac attttccatt aaaataaaga
     8761 caaagggaag gagtaaaatt gaaaatgtta actgatatga ttcaaaatcg tgaaattaaa
     8821 ataataaagt ttttattaaa agaaggtcat acaactgtaa aagcaatagc agatgattta
     8881 gatgtttcag aaaaaacagt ttcaaaatct cttaaagaag tagaagcagt gcttaaaaag
     8941 ataggtcttt ctttaataag aaaacctaag ataggtgttt atattgatgg agatttaaaa
     9001 aaaatacttc cttacctaga taataaagga agtcaaattc ctacaaccag agaggagaga
     9061 gttatctata tatattcagt acttttaaag agtaatgatt atattactat acaacaactt
     9121 tctgatgaac tatatataag tcgtggaact attgaaaggg atttagcaga agttgaaaaa
     9181 ctgctaaaaa acgaagggat ttccatatat aagaaaccta gtaagggaat aaagttaaat
     9241 ataagtgaaa gagataaaag acaacttact gctaagttta tacataaatt ttggagtaga
     9301 aactggtata ttaagcaaga gcaagaaagc ttttatcaag catttgaaaa gatacaagca
     9361 gatgtaaatg gtattttttc ggaagatgat ttatcaataa ttattgatat tcttaggaat
     9421 ttcagcagag aaagaaaatt tgagttctct gattatgaat ttcagtcatt agttattcat
     9481 ttggcaatag ctgtagagag aattagaaaa ggcgaatata tagaagaaag tttgtatact
     9541 ggtaatatag aaaataatca aatgtcaaat accaattatt tagcaaaaca attagaagaa
     9601 aaattttcaa tattattacc aaaatctgaa attggatata taaatttaca tttagtagcg
     9661 tctgaccaac atagagatac taatgaacta aaaaatactt caactaatcc ttgtgagaca
     9721 agtacaaata atatatcaaa tcttcgggaa attataaaaa atacattgta tgagacagct
     9781 tatgacaagg atttaattga tggattaact tcacatgtac attcatcaat aaatagactg
     9841 aaatatggac ttagtattaa aaatccatat gtaaatacaa tcaaacaaaa cttttcttta
     9901 tcatttgagc aagctttaga tttaaagaaa gtaattgaat ttatatatga tattaggata
     9961 aatgatgatg aatgtgctta tatagcatta cattttgaag cttttagaga aagaatgaaa
    10021 gaacttccac aaaatattag agtaattcta gtatgcagta caggattagg ttcatctagg
    10081 ttattgtctg caagaataaa aaaatacttt ccaatgataa agattgaaaa agtactttcc
    10141 gtacaagaat tagtatccag taaaattgat gcagatttag taataagtac tatttattta
    10201 gagttagaaa caattccaac catagtagtt agtcctattt taaatgaaag tgatataaga
    10261 accttggaaa gaaatataca aaatgtttca agttatactt ctaagaagta taaatctttc
    10321 agaaacttaa tctttgaaga aaatatattg ttgaatattg aagcttcaaa ctatgaagat
    10381 gcaattaagt atataacaga taacctaata aaaaaaggtt ttgcaaaaga aggtatagct
    10441 aaaagtgcaa ttgagagaga aaatttatct tacacttctt tccaatcaat agctacacct
    10501 catgctaatc ctagttatat taaaaaatct aatatatctg ttgcaacgtt aaaaaatcct
    10561 attacatggg gaaatgaaaa ggtggaagtt attttcttta tttcattaga aaatgacaaa
    10621 aacctagagc ttgatgaaat atatgaagtt ttttatgact tcattgacaa taaaaaaaat
    10681 ataaatgaca tattaagtgc aacttccaca aaggaagtgt acgatttgct agtggaggag
    10741 agtatatagt gaaaatatca gatgtattga aaaaagagca aattgttttt aatcttgata
    10801 caaatacaaa ggaagaaaca ataaaaaaat taagtagtat atttgttgaa aatgggattg
    10861 tggacaatga agaagaatat gttaagtctg ttttggaaag agaagaacat tcaactacag
    10921 gtattggtaa tggtattgct attcctcatg ggaaaagtaa tgctgtcaaa aaatcttcta
    10981 taatatttgc aaagactaag cataagattg aatgggatgc tttagatgaa aaaccagtag
    11041 atagtatttt cttattggct ataagcaata ttgataagga tagcaatcat cttgtactat
    11101 tatctaaatt agcaactaaa cttatggatg atgacaatgt agatgcacta aaattagcca
    11161 attcagaaca agaaataatc aatatattta gtgaagaagg agagatgtag aaatgaaaag
    11221 aaaaattata gcagttacag catgtgcaac aggagttgca catacatata tggcagctca
    11281 agcgttaaag aaagcttcaa aaaagatggg tcaccttata aaagttgaaa cacaaggagc
    11341 tacaggaatc gataatgagt taactacaaa ggatgcacaa attgcagaag tagtaatatt
    11401 tgcagtagat actaaagtca gaaatgctga acgttttgag ggaaaagaaa tactagaagt
    11461 accagttaat gcacctataa aggatgcaga gaaagtaata aatgatgcac taaagttaat
    11521 tgacactaaa taaatggagg gaaatgtaat gttgaaaaag atagtctctg agttaaaaaa
    11581 gcatgtctta actggaatct catatatgat tccattagta attgcaggtg ctatgataat
    11641 ggcgatatct cgtgtaggtg gttctttcta taatatacca gatatatggg atgctaaata
    11701 tgcagaaagt gctagtagta ttgttagatt attacatgat ttagatgggt ttggaggtac
    11761 tgcattagga ttaatgtttc ctgtaattgc tgcatttata ggattttcta tagctgataa
    11821 attagctatt gtaccaggtc ttgttggagg tatgattgct aaagatatag gtgcaggatt
    11881 tttaggagct ttggcagttg gtcttatagc aggatataca tgtttgttta ttaaaaatca
    11941 tgttaaatta ccaaaatcag cagcttcaat agtaccaata tttatagtac cagtatttgg
    12001 gacattaatc actgttgtat taatcaatta tgtgataggt ataccttttg ccacattaaa
    12061 tactggatta gaaaattggt taaatggatt atctggtaca aatcagatac taatggcagc
    12121 tattataggg gctatgatgg cagttgactt aggtggacca gttaataaag cagctttaac
    12181 aacatcactt gcattattaa caagtggaat ttatgcgcct aacactgctg caatggtagg
    12241 aatagttata ccaccactag gattgggact tgctactata ctagcaaaac aaaaatataa
    12301 taaacagtta cgtgaagctg gaaaatcatc tcttataatg ggtttaattg gagtaagtga
    12361 aggagcaatt ccttttgcgg tagaatctcc attaaaagta ataccttcat gtgtaattgg
    12421 aacagcaatt gcttcagcta tggcagttgg tttaggttca gtaaatgcaa ctccaattag
    12481 tggattttat ggatggttta cagtagagaa ttggccaatg tatgtgttat caatagcagt
    12541 tggaacagta attgtagctg gattagttat agtattgcgt ggaaatccag tgattgagga
    12601 tgatgaattt gaagatgatg attttgatga agcagagtgg gaatcttaaa atttaataac
    12661 tatttaagac gtgttataag ctatattcaa tatgaatctt atactaaata aagtgttaaa
    12721 cattcagttt agacttaaat ataaaagata aagaacagag gagaataaat gataaaagca
    12781 catattgtta atcatacaca ctgggataga gaatggtatt ttacttctgg agatgcatta
    12841 gtacttagtg aacaactatt tacagatgta attgatgaat tagaacgtaa tccagatgta
    12901 agttttgtac ttgatggaca actttctatt ttggatgatt atgtacaatt acacaaagaa
    12961 aaaatagaaa tcattaaaaa gttaataaaa gaagatagac tacatattgg accatggttt
    13021 actcaaactg atgcattctt tgctagagga gagtctattt taagaaactt aatgattgga
    13081 atttttgaaa gtaaaaagta tggtaagtat atgaaaattg ggtatttgcc agatacattt
    13141 ggatttaatg cacagatgcc tatactatta aagcatgtag gtcttgataa tatattcttt
    13201 tggagaggta tacatcttgg taaacaggtt caatcaccat attttaagtg gaaaagctta
    13261 aatggagaaa gttatatata tgcagtaaat atgccacatg gatatggaac tggtatgctt
    13321 ttagagcctt cattaaagta tgttaatgga cgcttagacc cagctattga ttttattaaa
    13381 tcatatactg atgtagatga ggtgttaata ccttctggga atgaccagtt aaatataata
    13441 ggtgatttca aaaataaaat taaagcgatt aatgatatcg gaaagtattc atatataacg
    13501 agttcatatc aagagttttt aaaatatata aagtccatag aatcactaga aagctataga
    13561 ggagaattta gagaacctgt attagcaaga atacataaat caattggttc tagtagaatg
    13621 gatataaaat tagcatgtga taaactagaa aacaagctga ttaaaagaat agagccatta
    13681 ttagtaattg ctaaaaagag taaaattgaa ataagcaatc agttacttat aaatacatgg
    13741 aaaaaacttt tggaaggaca agcacacgat agtttagctg gatgtgtaac tgatactgta
    13801 acagatgata ttcttcaccg tataagagaa gcaaatgaaa tatgtgacag tattgaaaat
    13861 actattgtaa aaaagatttc agaagggctt aaattatcta aaaatgatat tttagtattt
    13921 aatacagaag ctaagcgttt taatggatat aaagagatac aagttgtaag tgatagtaaa
    13981 aatatttatt ttaaagataa tttagatgct actataattg aagaaactta tgtaaaaccg
    14041 agagaaaatg tattagaaga aacacctgct ggtaatatat ttattgaaga accaggatat
    14101 tatatattaa aagtaagaat aaatgtacag cttcctgcac ttggttataa agttgttagt
    14161 tttgaatcaa gtgataaaga aatggtgtca cttaataaat ctaatgatgc ttttatatct
    14221 aatgataatt ataagataat atatgaagat ggaagcttaa atttacaact taaagatgga
    14281 acttacatat caaacttttt aacattaaaa gatagtggta atgcaggtga tacctatgac
    14341 ttttctccac taaaaaatga tgaggatata aaactttcgt ttgataaagt gtatacagaa
    14401 aaagctttag gatatgaaaa aatggtggtt aaaggtacta ctttactacc acttacgtta
    14461 gaagatagaa aaaacaaata tcttaatggg aaattaaatg taaaagtaag tattgtctta
    14521 tccaaagaaa atccattgat ggatgtaaaa ctttgtgttg ataatacgat atataatcat
    14581 agactacgtg ttcatataaa aactgatata aaagataata aaaacatagc atcattgcca
    14641 tttggatata taacaagaga aaatggagtt ttagacaact gggaagatat ttacagtgaa
    14701 atgcctatag atttagaacc tttggaaagt aatattactc taacaaacca aaatagaagc
    14761 tgtactatat ttactaaagg aataaaagag tatcaacata ttgaagatga aattgcattg
    14821 accttacttg ctactacaga tgaactagga aagcctgatt tattatatag accaggacgt
    14881 gcttctggtg atacaactaa aaaaggtcac ataagaatta aaacagataa agctcaaatg
    14941 cttaagaaat tagaattttc atttgcaata tatatggatt ctaaatcatt tgatgaattg
    15001 gaaattgcga atttaaccca caattatcta agggaatgtg taaattatca attacaggat
    15061 tacaatttct ttttatatcg tatagataat aagattcaaa aatcaattgt caataaaact
    15121 gtaagtagag aattagaaat aatatcttta ccagaaaact atcttatatc agcatgttat
    15181 ccttcttatt acagtaaaga taaatttata ataagagtgg aaaatccaag tagtaataaa
    15241 atgatattgg atgagcaaat atttaaaaat agaaatggga aaattgtaaa tgcaatagaa
    15301 gatgtagaag aggaacaggt atatgaaata gcaccttttg acgttatttc aatattattg
    15361 gatttataag tgtaaaagta agtttattgt ttaaagtctg acagatatta atctgccaga
    15421 ctttttaatt tgtataaatt tgggtaaata ttaatgaatg gttttagtat ttaagtatga
    15481 atttagtatt taatatagaa tttattgaag tagaataatt agtttaacag gataagcagt
    15541 ttaaataaag aattattata ttagagtgat tttatatata atttaagata ataaaatttt
    15601 aataaaatga gttaaagatt gtatattaat agagttgatg tagttacaaa aaagttacca
    15661 attgttatgt atttacaaaa ctgatatatt aattacataa aatctaaaat agtttgtaat
    15721 aggagtatat ataataaaaa ttttaattaa ataaagctag gtatatttat aatattaata
    15781 aatatattgc tataaaagtt tgtttaataa attaaataaa gaaaagagga gaataaaatg
    15841 tacaacatag atgatatgat atataattgt ccagtagaag ctttatcaaa tattttaggt
    15901 aaaaaatggg tggctataat tatatggaaa atacaagaag ataaaaaacg atttggagag
    15961 ctacaaagag ctataccaga ctgtagtaag aaaatgttag ttcaacaact agatttttta
    16021 attgaacagg ggattatagt caaccaaaaa aagttggtta ataatgttgt agagtcaaca
    16081 tattttttaa gtgaatcagg attacgattg cttcctgtca tggaaaaaat gattatgtgg
    16141 agcaataaaa atttaaattg tgataaatac taaaatttac tctgtaagta atgaaaattt
    16201 aattaagcta tgagtagaaa aatgtattaa aattacataa ttttttagtt agattttaat
    16261 ttagaaatgg gattgtaata ttggaaataa aataaggagg aattttaatt tgcaaaatgt
    16321 aaatttattt ttagtattta tagaaggtat agtatcattc ttttcaccat gtatactgcc
    16381 aattttgcct atttatttga gtatattgtc aaatagtagt gtagaaaatt taaaagaagg
    16441 aaagacgagt tttataggaa gttctttatt taaaaatact atattttttg ctcttggaat
    16501 ctcaacaaca ttctttatat tgggttcatc agtaaaagta ttgagtatgt tttttaatga
    16561 aaataaagat ttaataatgt ttattggtgg gattataatt attatcatgg gattatttta
    16621 catgggaata attaaatctt caatattgaa tagagaaaaa agatttaatg ttaagtttaa
    16681 agaaatgaaa gcaataacag cttttatatt aggttttaca tttagttttg gatggacacc
    16741 ttgtattggt ccaatattgg catctgtatt agtcatggtt tcaagttcaa gtaatcatct
    16801 tagtgcgaat cttttaatag cagtatatac tattggtttt attttgccat tcataattac
    16861 agctatgttt tacagtaaac tatttaaaac tatagataag ataaaatcta acatggaaat
    16921 tattaaaaaa ataggtggga ttatacttat agtatcagga atattaatga tggtaaatgg
    16981 atttggaagt ataagcaaac actttaatac gtcacaaaat agtaaaattg aaagcaagca
    17041 agaagaaaat aagcgagaaa attctactga taaagaagaa aattctgatg gtaatgatag
    17101 tcaaaaagac tctaataatg acaataatga taaagggtct aatgatgaag atagaataaa
    17161 gtctattgat tttactttaa cagaccagta tggtaaaaca cataaattaa gtgattatga
    17221 aggcaaagtt gtattcttaa atttttgggc tacatggtgt cctccatgta aagaagaaat
    17281 gccttatata gaacaactgt ataaagacta taataaaaat aatgatgatg tagtaatttt
    17341 aggagtagct tcacctaatt taggtagaga aggttcaagg gaacatgtag ttaacttctt
    17401 aaaagaccaa ggttatacat tccctgttgt acttgatgaa gatggagcat tagcatatca
    17461 atatggaatt aatgcttttc caactacatt tataattgac aaggaaggat atgtaactca
    17521 gtatattcca ggagctatgg ataaggcaac tatggcatca tttattgaaa atcagagaaa
    17581 taaataaaag tatttaagta taagtttata aatcaattta aggttacaaa aaagtgccta
    17641 attgtaccac ctaaataatc ttgttatagt aaaagtataa acaatacaag aaagggttga
    17701 attttatatg aaaaaaacat ttgatgctat aataatagga tttggaaaag gtggaaaaac
    17761 attagcaggg gatttggcaa acaggggatt aaaagtagct ttaatagaaa agtcaaacaa
    17821 gatgtatggt ggaacatgtg taaatgtggc atgtatacca acaaaaagct tagaaaattc
    17881 agcaaacagc gtaaaaacaa agaatataaa ttcttgggat gaagtacaag ctgaatatga
    17941 gaaagctata gataaaaaag aaactttaat tacaaaactc agagaagcta actataataa
    18001 attaaattca aatgagaatg taactatatt cacaggaatg ggaactttca ttgatgaaaa
    18061 aacagttcaa gtcaaaactg aaaatgaaat ttatgagtta gtagcagata atatatttat
    18121 taatactggt tcaagaccat ttatacctaa tattaaggga attgaaaata agaatatagt
    18181 atatgatagt gagtctctta tgaatctaag aactttacct aaaaaaatga caattatagg
    18241 agctggattt ataggtttag aatttgctgg tatttatagt tcatttggag cagaagttac
    18301 aatattaaac tctaataatg gcattttacc aaatgaagat gttgaggatt cagaagaaat
    18361 aataaaatta cttgcaaaga gaaatgtaaa aattgttaat aatgcaaata taaaagagat
    18421 taaagaagtt tctgaattag caatagtaga atatgaagta gatggtaaat ctaaagaatt
    18481 aacaagtaat atgatacttg tagctacagg aagaaaagca aatactgagg agctagggct
    18541 agaaaatgca ggtatagaat taaatgaaag aggatttatt aaagtctcag aaactcttaa
    18601 gactaataaa gagcatatat gggctatagg agatataaat ggaggtccac aatttactta
    18661 tatctcattg gatgattatc gcatagttat taatcagttg tttggagata agactagaac
    18721 aactaatgat agaaagaata tcccaaattc tatatttata agtccagcat tttcaagagt
    18781 tggtcttaat gttaaacaag ctaaagaaaa gggatatgaa gtattagtgg caaagatgcc
    18841 agtagaagca attccaagag ctaaacagat tggcaaggca gatgggttta taaaaatagt
    18901 gatagataaa aaatccaata agatacttgg agctagtatg atatgtgaaa attcaagtga
    18961 gattatccat cttattcaat tagctgtaga tttagaagta gagtatacat atttaagaga
    19021 ccgtgtttat gcacatccaa ctatgacaga agctcttaat gatattctat ctcctaatat
    19081 gataaaagaa gtataacata agtttaatat ataaacctaa aattatcatt ataagtagat
    19141 tttagaaaga ggctatctca aaaaatagag ataagtctct tttttactgt ttaaatgata
    19201 aaaatatatt tgattttata aaagaagatt atcttatgaa ctaaaaagtt tattttaaaa
    19261 cactacctat ttatgttata aacttataaa tataatatag aatctattat tctgtattat
    19321 taagtggggt taagcgttat aattaattat aaatattgaa taatctaaat ggataagata
    19381 atggtattaa aaattatctt aaaaggtgaa ataatctgta tttaatgtag tacagttata
    19441 tctttaaagc aaggtggtga aatgatataa ataagatatc taaaagaggg tttgatgata
    19501 gtgataaaag atggttttca agtaaagaat taacaagact tataaagtct aaagaagaaa
    19561 tagaatggtt attaaatagg gattacaaaa tagacccagt cgttactttt gttggtgata
    19621 gatatcagtt ttctataaga caaagagatg cattaaaaag ggcagtctgt actgatgaaa
    19681 aaaatattat aagacaatca aaaaggcttt ctctagataa aatcaaagaa gataccgtaa
    19741 atatagatgg ctttaatttg ataatatcca tagaggtagc attatctggt ggtactctag
    19801 taataggaag tgatggcaat ataagagatt tagcaggtct taggggaacc tataagatta
    19861 tagataaaac agaagaagct ttgaatctga taggtaaatt ttttaataaa tacaatgcac
    19921 agaatcttaa attttattta gatgctccag tttctaattc aggaaattta aagtatagga
    19981 tattagagca tgccaaaact tggggaattg aaactgaagt agaattagta aaaaatgcag
    20041 atgttgtatt ggaaaaatta gatagagtag ttagtagtga tgcagtcata gttgacaagt
    20101 gtataagtta ttttaatgtg gctagaggta taattgaaga gtatattaaa gaatgtaata
    20161 tcataaattt aaataaatag ataaatatag gtgagaatat ataagtatat gtagcaatat
    20221 tcttagtaat acaagtaaag tttgaaaata ggagaacgaa tacatatcga atttcataaa
    20281 atgttgtaaa aagacatatg agaggatgga tatgtgtgaa attaactaaa ctaaatatta
    20341 aaaaatatag agcacctata tataaatatg ctttagcaaa tgtaaatttg aggtctgcta
    20401 aatctactaa ttcaagtatc ataacagtaa tacctcaggg agccaaaatg gaagtattgg
    20461 atgaagaaga tgactggatt aaggtgatgt acaattctca agaaggatat gtgtataagg
    20521 atttggtatc agtaagcgaa tatgcttgga gtaacttaaa cttaagagaa gataaatcta
    20581 caacttccaa tataattaca gtaattcctg aaaaatctag ggttgaagtc cttcaagtag
    20641 atggagattg gagtaaggtt gtatatgatg ataaaatagg atatgttttt aattattttt
    20701 tatcaattga tgggaataaa ccaaatgaat tagattataa gtatttttat actgacatga
    20761 ttaaatttgt taacgaaaat aacattaaaa gtactagcga ttatctaatt gtaacagacc
    20821 ttagaaataa atatacttat atttttaaga aagataatgg aggatgggga caactttaca
    20881 agtggcaatg tactataggc aagcctgaga ctccaactat aacaggtata ttttatataa
    20941 gtggaagaaa accttctttt ggaacggatg agtattcagt taagtatgct actagaatta
    21001 aaggtggata ttactatcat tctgtactat atgattcaac aggaagttat ataatagatg
    21061 gcagacttgg tgaagctctt agtcatggat gtataagatt aagtacggaa aatgcaaagt
    21121 ggatatatga taatatacca gatacaacaa cagttataat tcattaagaa aaattttgag
    21181 ttttttattg ggataataat tcttaaatta atagaattat atattaactt ctcataaaga
    21241 gtactattta aaaagtgtaa taagagagtg ctattaacaa aacaaagata ttttgaggca
    21301 ctctcttata aatatacata attaagatat tttcataaac aattctataa tcttatcttt
    21361 atcaaagaaa acaggattag caccagcaca agcatctttc atagagtttt ctgcaagcaa
    21421 ttcaaaatta ggattatcaa cacctacatc ttttaaagat tttgggattc caacctcttc
    21481 agaaagagct tttatcttat ctattacaaa gtcaacacac tctttatctg atttattatt
    21541 tacatccatt ccaataactt cagctattgg tcggaatttg gcaggggctg atttagcatt
    21601 ttcctcctca actattggaa gtagcatggc attacatact ccatgaggta aatcatataa
    21661 acctcctaat tgatgagcca tagcatgtac atttccaaga cctgcattac taaaggcaat
    21721 tccatttaaa aaacaagcat aacacatttg ttctctagct tctatgtcat taccattttt
    21781 aacagctctt ggaaggtatt taaatatctg ctttatagca tataaggcag ttgaatctgt
    21841 aacatcataa gcaccattag ctactacagc ttctatagca tgagttagtg catccatacc
    21901 agttgctgct gttaaattag atggtttgct aatcattaat tctgggtcat ttactgtcat
    21961 tgtggctagt gaattggtat caaccattat catctttaca tgtctgtctt catcagttat
    22021 aacatagttt attgtaactt cagcagatgt accagcagta gtagttatag ctatgatagg
    22081 aagacatttt ttagaagttt tgttgatacc ttcatagtct ctaatatctc ctccattagt
    22141 ggctaatata gagatagctt taccacagtc ttgtggagaa ccacctccta tagttataac
    22201 gaagtcgcaa ttttctgatt ttaatatttt aagtccttta ttcacattag ttacagttgg
    22261 attttgttta acttcatcat aaacaacaaa gtctacatct atctgagata aaatatctgt
    22321 tacttttttt acagtaccat ttgaaactag aaatttatca ctaacaataa atgctttttt
    22381 agttcctaat gatttaattg aatctttagc ttcattaaga catcctttcc caagtaaatt
    22441 tattggtggt acataataaa tgctcataac taaaatctcc ttttattttc attgattttt
    22501 atgtaaaaat atatatctaa caattttcac taacttactc ataatatatc gtatcttaga
    22561 ttgagatagg attgttggtt cttatataag aacttaattc gtaattacga ttatgattag
    22621 aatttaacat agtattttta tcatgtcaat aaaaatataa aaaatattat aaaaaaaaca
    22681 aagttactgt agttaaatca gcaactttgt ttttatagta taaatcaaat aaatacatta
    22741 gtttaaaaca aaatttttaa ttattatgtt ttgatatctt ccatatacag aatattaatc
    22801 taatttatta caatgtcttc attacgttct tccatcattg attgaatatt tttctgagca
    22861 tttaagagta atttttttat cagttctatt ttttcgtcat tagctctaaa taaaggcagt
    22921 gttacactaa cagcagctct tatacatcca ttttttctta ttggtacggc aagacatttt
    22981 aggtgttcca ttgattcctc acactctgaa gctatttgtg ttctttgaac ttcaagaagc
    23041 tggtcgtata aaacatcaaa atctgtaatt gatttttctg taaaaccttt tagaccatct
    23101 ggatataagc ttattagttt ttccttgtca aaatcacata aaagagcttt tccaagacct
    23161 gtacaattag caggtatttt ttttccaaca taagaaataa gtctaattgg ttctggagaa
    23221 tctacttttc caatataaag aacttcatta ttgtcaagta taccaagctg acaagtttca
    23281 gaacaggaat taactatatt agtcatttcg tcaattatat agttaaaaat agttcttttt
    23341 tcaaaaaatg aagaccctaa tgtgtaagaa cgtatcccta tagaatattt ggatgtgttt
    23401 ttatcaatag taatgtattt tctagcatta agtgtatgaa ctataggaaa gatgctactt
    23461 tttggagcat tgataacatc tgctatttct gtaagagtat atccattttc ggattctgct
    23521 aatgcttcta aaatatctaa tatcctagct gttgggcgat gattatctga agtcataaat
    23581 ttccccctct ttttattaat ttaaagtatt agaatatgtt gattttagca cactatacga
    23641 tattttacaa tgtagaaaat aaatatgtaa atttatatat aaataagctc ttgatttaga
    23701 tacagataga tattttttta gactgtacat taaggaataa ctaatttcca aaatgtacct
    23761 aa
//
LOCUS       DAYASV010000052        23636 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYASV010000052 DAYASV010000000
VERSION     DAYASV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.052"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            199..1275
                     /locus_tag="ACGI68_001983"
     CDS             199..1275
                     /locus_tag="ACGI68_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0138421.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1378..2220)
                     /locus_tag="ACGI68_001984"
     CDS             complement(1378..2220)
                     /locus_tag="ACGI68_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFH0138422.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2404..2976
                     /locus_tag="ACGI68_001985"
     CDS             2404..2976
                     /locus_tag="ACGI68_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0138423.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3139..3411)
                     /locus_tag="ACGI68_001986"
     CDS             complement(3139..3411)
                     /locus_tag="ACGI68_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138424.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3538..4410)
                     /locus_tag="ACGI68_001987"
     CDS             complement(3538..4410)
                     /locus_tag="ACGI68_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0138425.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4706..7813)
                     /gene="ileS"
                     /locus_tag="ACGI68_001988"
     CDS             complement(4706..7813)
                     /gene="ileS"
                     /locus_tag="ACGI68_001988"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFH0138426.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7906..8138)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8313..8840)
                     /locus_tag="ACGI68_001989"
     CDS             complement(8313..8840)
                     /locus_tag="ACGI68_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFH0138427.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8875..9660)
                     /locus_tag="ACGI68_001990"
     CDS             complement(8875..9660)
                     /locus_tag="ACGI68_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HFH0138428.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9670..9927)
                     /locus_tag="ACGI68_001991"
     CDS             complement(9670..9927)
                     /locus_tag="ACGI68_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFH0138429.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9946..10404)
                     /locus_tag="ACGI68_001992"
     CDS             complement(9946..10404)
                     /locus_tag="ACGI68_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFH0138430.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10444..11148)
                     /locus_tag="ACGI68_001993"
     CDS             complement(10444..11148)
                     /locus_tag="ACGI68_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFH0138431.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11158..11793)
                     /locus_tag="ACGI68_001994"
     CDS             complement(11158..11793)
                     /locus_tag="ACGI68_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HFH0138432.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12174..13214
                     /locus_tag="ACGI68_001995"
     CDS             12174..13214
                     /locus_tag="ACGI68_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFH0138433.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13677..15035)
                     /locus_tag="ACGI68_001996"
     CDS             complement(13677..15035)
                     /locus_tag="ACGI68_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008624114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFH0138434.1"
                     /translation="MVNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREK
                     IYNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDK
                     AAEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMD
                     AVKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGV
                     SGPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSL
                     APTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIP
                     VSEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGM
                     INNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSF
                     KN"
     gene            complement(15058..15327)
                     /locus_tag="ACGI68_001997"
     CDS             complement(15058..15327)
                     /locus_tag="ACGI68_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0138435.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15617..17572)
                     /locus_tag="ACGI68_001998"
     CDS             complement(15617..17572)
                     /locus_tag="ACGI68_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138436.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLKDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWYFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKLVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17755..19230)
                     /gene="spoIVA"
                     /locus_tag="ACGI68_001999"
     CDS             complement(17755..19230)
                     /gene="spoIVA"
                     /locus_tag="ACGI68_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HFH0138437.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19402..20697)
                     /locus_tag="ACGI68_002000"
     CDS             complement(19402..20697)
                     /locus_tag="ACGI68_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFH0138438.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDESGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20881..22326)
                     /locus_tag="ACGI68_002001"
     CDS             complement(20881..22326)
                     /locus_tag="ACGI68_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0138439.1"
                     /translation="MSLNKFEIGGITVKKKIGFWDLVFMNISALFGIRWIAKSTASSF
                     GLGLGSIPVWLLFAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLN
                     WTSKIFWYSSFLTFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGK
                     FFTNTGALGSTIPAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAII
                     FALSGAETTANFITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAAST
                     GILDALAKVAQDLGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQ
                     LTVTNKYNIPSNAVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFT
                     SYIRLRKTRPNEERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYE
                     IEMIGGGLFVIITGLMLWKNYEKKISNNSGC"
     gene            complement(22342..23481)
                     /locus_tag="ACGI68_002002"
     CDS             complement(22342..23481)
                     /locus_tag="ACGI68_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0138440.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
BASE COUNT         7511 a         4181 c         2672 g         9272 t
ORIGIN      
        1 acttaattta ttacttttat agaattttca caataaaaag tcattttact tccgtattta
       61 atgcaaaagt agaaattaaa cttaatataa aaaatatata acaaattttt tttatatttc
      121 tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt attgaatttt
      181 gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga acaaatgaaa
      241 aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta tcttacagga
      301 gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa tggcaataat
      361 aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga aatggtttgg
      421 tttaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa aaatgctact
      481 atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga attaggtgga
      541 ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg taaagatgaa
      601 gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat ggaacaactt
      661 aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt atctaaaata
      721 tatgaagacc taggtactga tggattttca tttgacccta ttataggttt tggacctaat
      781 ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga tgcaataatc
      841 ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac tgtattctat
      901 aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc taataagaga
      961 gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc tgctcgtgac
     1021 tacataactg aaaagggata tggacagtac ttcactcata gaactggtca ctctatagga
     1081 cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt acaaccagga
     1141 atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt tagaatagaa
     1201 gatttagtac ttgttactga agatggttgt gagatattaa acaaacatga caaagaaata
     1261 tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata taaaaatgtt
     1321 ttaaacattt ttatatggct ttaatttctc aatctatgat ttatttcaat ccattattta
     1381 tattgcagtt attaagattg caccctttga agtatatgtc cccatcactg aaccaatttc
     1441 acagattaca acatctttgt attttacatc cttttccata acttccctta ccttttgagc
     1501 cttttctggg caattagcat gtcctataaa taaaaccttt tcttctttat ctttaatttt
     1561 tgactctact aaagtaatca gctttttcaa actgtttcct tcacctcttg ccttatctat
     1621 aggagttaca acgccatctg caacttgtat tatcgcctta aaatttaagg tatttataat
     1681 ttttcctgct attggattta ttctaccacc cttaatagca ttttctaaag tttcaagtgt
     1741 tccaaaaaat ggaggattat ctttatattt atttattatt gaaacaatct cttcaatttc
     1801 ttttccttct tttattaata ttgctgcttt taacactaat tgtcctattc caattgaccc
     1861 actaagtgaa tctataacct caactttctt gttatttcct tccttttcaa acatatcttt
     1921 tgctaaaagt gcactattat aagtcccaga taatcctgaa cttatattaa tcactaatac
     1981 actttcttcc tgacaatggt atgcttctaa aaatttatca ggagaaggac atgatgtttt
     2041 tggtagtact ttactctcac tcatttttct atagaaagtt tcattatcta tttcctttcc
     2101 agatatataa ctttcttctc caaaagatac atttaaacca acaacttcta tgttgtattt
     2161 ttctattata tcatcaggta agtcacatgc actgtccact atcaatttta ttttatccat
     2221 attaacctca ctataagata ttattttatt tattcagtcg gtcgtacgac cgatttaatt
     2281 attattttat atattgaatg aaaaaaagtc aatagatata gattctcgct ttattgacaa
     2341 taaatatcca taagcttata atgttattaa tttgttataa atatacatgg aaaggtaggt
     2401 tttatgaaaa caaataatat gaaacaggct attttatcat ctgcaaccaa tttaatagcc
     2461 aaaaatggag ttcaaaatac tagccttgct gatattgcaa aagatgtaaa tataagtaaa
     2521 ggtactttgt attatcacta cgcatctaaa gatgatatta tatatgatat tgcggatact
     2581 catttagaag ttataactag tgctatacta aactgtgtaa aaaatgtcaa atcaaaaaat
     2641 tctcaaatag aaatggttaa cttaatctta gagaaaatat ctactataga aagtcgtggt
     2701 agggtacata tgtatcttat ttgtgaggca attacttcaa ataatgattt aaaggaaaga
     2761 ataagattaa aatatattga atggagaact actcttaaat ctgaaattgt tgaatcacta
     2821 gaaaaatata atagtagcga aaatgaacag gatgctgaat cattttcttt tttactcatg
     2881 tcaatagttg atggtttagt tgtccaaagt ttattaaaaa cagaaaaaat accttatgaa
     2941 aatatagctt cttttttagt taatcattgg atttaaactt aatacttaga taattaataa
     3001 aagtgaacta tttaagttat ctactttaac agttcacttt cttatttatt ctcaataatt
     3061 tttacttaaa acattcaatt tttatttaaa ccttcatgga atcacaaaat aaaaatctct
     3121 tataaattta aaatgttttt atttatgttc atgtgtatgc ccatctgaat gttcatgacc
     3181 atgtgtatgt gtatgctcat ggctatgctc atgagtacct gaaaaaccat catcatgatt
     3241 atggtcgtga cctacaccat ttacatgctc atgttcatgc ccatgactgt gagaatgttg
     3301 atgtgaatgt tcatggttat ctcctccaca gcatccttct cctccagtcc cacaacaccc
     3361 acttgtatgt ggattatgat gatttcttac tacaaatctc tctcttaaca taattcttat
     3421 acctcctagt atattattga gtacttcatg taccccatta actacagtat acatctaact
     3481 aggtataatc tcaatacata tggcattttt taacatatat aattaacata tataatctta
     3541 aagaaattta tccttaaaac ctccaccttt tacttcttca acgttattga cagaaataaa
     3601 agcttcacta tccatatcat aaattaaatc tttaaattta cctatttcat ttgcagatat
     3661 tatgcaataa atcatctttt tcttattttg tgtatatgca ccttctgcct ccaaaaaagt
     3721 gactcctcta cccattttat tcattatcac tttggatatt tcatcatatt tatttgatat
     3781 taaaagtata gatttttttc tatcaaagca atcttttcct atatccatag taaaagaaat
     3841 tatatacatt gtaattaaag tgtacatcac caatttagct ccaaataaaa aagctcctgc
     3901 acatactaca aaaaaattaa taaataaaaa tgtatttttc ataggtatat catactttct
     3961 tttcattatg gctgctatta tatcaagtcc tccagcagta gctcttgctt taaaagttaa
     4021 ccccatacct ataccagtta atgcaccacc ataaatacac tgtagcatag tatcatctat
     4081 tgggaaatat ttagcaatat cttgtgttag ccccagtgct agagaaaata gtaacatatt
     4141 gacaaaactt attagcaaaa atttattatc aaaatacttt ctagctataa taaatattgg
     4201 tatgttcatc aagaaacttg atattccctg attaatacca aataatttgt ataatataac
     4261 acaaattcca gctactcctc cacttaataa cttggctggt gctagatata aatttattcc
     4321 taaggttgat agaatcatac ccaatataat aagacaatat tgtaaaaata tattattatc
     4381 aatacttagc tttttacttt caccatacat caaaacgcac ccctttgcat aataaataaa
     4441 ccaccctgca taacacacga taaatttaca aataaattta tcagaagtag tttcctaaaa
     4501 atattattca tcctcactta aacgtttatt cattaatatt ctatcacatg acataaataa
     4561 tttatatgat ttatatagat ttatttattg taaattttgg aatattaaaa cattttattg
     4621 aatagataat tatattacat aaaaaatccc tatagaaatc tataaactaa tttctatagg
     4681 gattttggtt aactaactat tatttttatt tttttataac ttttatacct gtcatatggt
     4741 catttaagtt ttgttcctct aaagatttat catctactct gtttatttct aaagctaaag
     4801 tttcagactt gatatattca ttaaaatggt caacagcctt agcaatttca tcatcaccac
     4861 agtattctat tactatatta tctaatactt caaagttatt agattttctt aattgctgta
     4921 ctttagatat aaattctcta gcataacctt cattaattaa ctcttcagtt agcttagtat
     4981 ctagtataac aaaaagattg ttttcaacag atacattaaa tccttcctta gcagttatac
     5041 ctattaatac taaatcctta ttaaattcaa aagcttcacc atctaaatca actgttaaag
     5101 tctcaccttt ttcaagttta ttagcagttt catgagcatt taatttactt aatgctccaa
     5161 cgaataaatt catcttagaa cctaataatg gccctgcttc cttaaagttt ggttttaaac
     5221 taaagttcat atactcatct aaatccttag caaatattac ctctttaaca ttaagctctt
     5281 cttttattaa atcaactaca tcacttattg tatcttcaaa cttaccatct actaacactt
     5341 tttgtaatgg ttgacgaact tttattcttt ctacttctct tgaagctcta cctaaagtaa
     5401 ctaagttttt agctaaatcc attttttctt ctaatttagt atctactaac tcttcattac
     5461 atttaggata ttcagctaaa tgaacagata cttctcctgt taagtttcta tacatttctt
     5521 ctgacatata tggtgcaaat ggagctatag ctctacaaag accatgtaac aattcataag
     5581 ttgtattgta cactgctttt ttatcttctg ttaattcagt tgcccagaat cttcttcttg
     5641 aacgtcttat ataccagtta gataaatcat catttataaa atcttgtatc attcttaaag
     5701 ttttatttaa ctcaaatata gctaaatttt cttctacatc tttcattaaa ttattgaact
     5761 tagataatat ccatctatca agttctggtc tatctttata ttctacaaaa aattcagttg
     5821 gatttatatc atctgtgttt gcatataaag taaagaagtt atatacattc ttcatagtac
     5881 ctataaattt actttggatt tcttttaatc catccatatc aaatttagtt ggagtccatg
     5941 gtggagaaac atatagtaaa taccatctta atgcatctgc tccatattgg tcaaataatt
     6001 ccattggatt tactgtattt cctcttgatt tactcatttt cttaccatct ttatcaagta
     6061 caagatctgg aactaaaaca ctcttatatg gtgcttttcc taatacaaat gttgatactg
     6121 caagtaatga gtagaaccaa cctctagtct ggtctatacc ttctgatata tagtcagctg
     6181 ggaacaattg gtcaaagttt tccttatttt caaatggata gtggtgttga gcaaatggca
     6241 tagccccact atcaaaccaa cagtctataa cctctgttac tcttgtcatt ggttttccac
     6301 atttttcaca ttttaagtgt atatcatcta catatggtct atgaagttca actgtttctg
     6361 gatttacatc ttctatagct ttttcagcta attcagctct tgaaccaact gaatctgtat
     6421 gaccacattc acatctccat ataggaagtg gagttcccca atatctagtt cttgatattg
     6481 cccagtcatt tagattttct aaccagtttc caaatctctt ctctcctaca aaacttggga
     6541 accattttac accattatta ttttctatta atttatcttt aagtttagtc atctctatat
     6601 accagcttgg ttttgcatag tatactaatg gagtttgaca tctccagcag tgtggataat
     6661 tatgaactac tttttctttg ctatataatt tattttcaga agctagccat tttattattt
     6721 caacatctag cccatcttcc attacaaatc taccttccca aggagtagat gtaaatttac
     6781 cagtttcatc tactggttga ataactggta aatcatatct tttacctgtg ttgtagtcat
     6841 cttcaccaaa tgctggtgct gtatgaacta taccagttcc atcttcagta gttacataat
     6901 ctcctactgt aataaaaaat gcttttttgt caacttctac aaaaggcatt aattgttcat
     6961 attctaaacc ttctaggtct ttacctttca tttcttctaa aacttcataa tcttcaccta
     7021 gtactttatc agctaaaggt ttagatacat aatatacctc atcacctttt ttaactttta
     7081 aataatctac atcagcattt actgttaatg aaacgtttga tggtaaagtc catggagttg
     7141 ttgtccatgc taagaagtat tcatcagcat cttttttctt aaatttagct ataactgtat
     7201 tagtttttac ttctttatat ccttgtgcaa cctcatgact agcaagacca gttccacatc
     7261 ttgggcaata aggaagtatt ttatgtcctt catatatata tccttctttg ttgaatttgt
     7321 ttagtatcca ccatacagac tctatatagt tattatctaa agttatatat ggattatcta
     7381 aatcaacttc ataagccatt ctctcagtca ttattctcca ttgtttttca aatgagaaaa
     7441 ctgactctct acatttttgg ttaaattcag ctattccata ttcttctatt tgttgcttgc
     7501 tagtaagacc taactctttc tctacttgta tttcaactgg taatccatgt gtatcccaac
     7561 cagcttttct ctttacttga taaccagaca ttgttttata tctacaaact gaatctttaa
     7621 gcgttctaga taaaacatgg tgtactccag gatttccatt agctgttgga ggtccttcat
     7681 agaatacgaa acttgggtca tctttccctt tttctaaagt tttttcaagt atatttatgt
     7741 ctttccaata atcaaaaact tgtgcttctg cttgttttac agaactgtct accaatggct
     7801 taaatttcgc catgtaaatc accctttcct actaaatatt ataattccaa aattttttat
     7861 tctttcacat aaatttaaat actaaaaaac tcgccccctt taaaccaaag ggacgagtta
     7921 taaatcgcgt taccacccta attctatatg taaactaaat actagctaca tataacactc
     7981 taatagtata acggtactaa ccggcacagc ttagtagaat ttaatcgttc agcctgcagc
     8041 tcaagagtga tttttatctt tataaattca ttggttctca gcatacccaa ttctctgtaa
     8101 aattatctaa agataaatgt ctctatcaac gcttttaaaa atttatctgt gtttatatga
     8161 aattctataa caaataattt gaatagtcaa tatatttttg aaaaatataa taatttctat
     8221 ttttcttaca tttttatcct atttttaatc tttgttatag atttctcaaa ctcacacaat
     8281 tattctacat aaaaaataaa caaattacaa gcttattcta aagttgtagc agcttcatca
     8341 ttactataat tatacactgt tgttccttca aatgcattta cacatttttc atctacatca
     8401 taaaaaatct catcaacact tcttatttca tcttctaaca gtgatttaaa tttatttcta
     8461 aatatcttaa attctttgac taaagcatca tactcttttc ttatctctat aacacggtta
     8521 ttagcttgtt ctataatttg tctagctttt aaatcagctt cttcaacaat tatcttagcc
     8581 tttttattag ccgcgctaca agtgtcttct gctgcacttt gagcagttat taaagtagct
     8641 tttagtgttt cttcaatatt ttcatacttg tttacttggt cttgatataa ccctaatttt
     8701 tctttaagag ctgtattttc cctacataga gattcataat cttccttgat gatatctaaa
     8761 aattcatcaa cctcttcatc tctataccct cttaaacctt ttttaaactc tttattttct
     8821 atctcaattg gagttagcat tacatttcct cccagctata atttaaatta gtccttatac
     8881 aattaatctt gcttgaactt ttatttttcc ttttttagta atatctccta tttgtgttac
     8941 cttagctctt cctttgcctc taatagaaat caaatcatcg ttttttacta ttttagaagt
     9001 tgaagttatc ttttcataat tgacatggac tttttctcca tttatgtatt ttgcactatc
     9061 ttgtctagat atattgtaaa taccacttat cacacaatct aacctatctg aagatactgt
     9121 aaatgatact tctttatatt ttgaagaact attaacaata ttttctctgg aaatttcctt
     9181 tacaattaca ttatttcttg aaaccttttc taagtttaca attataaaat cgcaaatatc
     9241 actacttaca actacttgac aaaagctatc atgtataatt atatctccaa ttttttctct
     9301 ttttatacct agacccagta ttgaccctaa gtaatcttta tgagatatat ttttaaattt
     9361 gaaattccct tctatttgta aaaatctaag agtatcttct atatcttcat attccatgta
     9421 aaatggataa ataaatacag tgcttctttc agcttgttca taacctccat ccacactgta
     9481 ttttatttca tttgtagaat ttaatattgc tacagcattt ttcacttcat atggattaag
     9541 aaactccgta gacttaacat catagttttt tatacaacta ttagccttgt ctatcacttt
     9601 aaacatttta tttttcaagt ctatatcctt tatgtgattt gttagtttta atttatccat
     9661 cctatcacct tagaatataa ccaatactaa ttgtcttagg aacatcaata atactattgc
     9721 aattaaagga gaaaaatcta taggtccatt tgtatattta tacattatag accttattgg
     9781 cccttctatt ggctctgtaa gattttccaa aatatcaaaa attttacttt gtcttccatt
     9841 tggaaaccaa gtcatcaagc tttttataac tatcatccat gctattatat ctaaaagata
     9901 ataaagtgca attcttattg ttcccatata ttaaccgcct ttttactatt tattttgcca
     9961 agggaagaat gctttacttt ctaattcttt tttcatattt gcatctatat ccacattatt
    10021 aggagctagt ataaatattg atttagaaac cttttgtata cttccatcta agacataaac
    10081 tgccccattc ataaagttaa atatagattt acgaacttca gaattagtca atccttccaa
    10141 attcactata actgcccttc tttgttttag atggtctgct atagttgcag cttcatcata
    10201 aaccttaggt tcaactatta caactttcat ttggctagtt gtatgtaaat ttacaatttt
    10261 atttgctttt gcagtactaa agccactgtt catttcttct tcttgaacta tatcgtccat
    10321 gccactttca tattcatctt caatgtaatc ttcttcttca tcaactatcc aattcttaaa
    10381 tttagatatt attccatttg acattattaa cttcctcctt aaaatttatt caaatttgag
    10441 taattatttt ttattgtagt ttctttctcc aaagattgat gttccaactc taacaatggt
    10501 agccccttcc tctatagcaa tttcataatc gtgactcatt cccattgaca aaacatccat
    10561 ccctacatta gggatgttta gttcttttat ttgttttgat aaatctttaa gacctttaaa
    10621 tacacctctt atctcatctt catcttctat aaaaggtgcc attgtcatta gaccctttat
    10681 ttttatattt ttatattttt ctgacacttc tttgacaaag tctacaactt cttctctatc
    10741 aagtccatgc ttagactctt ctttagatat attcacttgt actaagcaat ctatttctct
    10801 gtttatttgt tctgcacgtt tttgaatttc ttcgcataat gcaattctat ctaaagagtg
    10861 tatcatacaa actttatcga ttatgtactt aactttattg gtttgcaaag ttcctatcag
    10921 gtgccagttt aatttatcac ctataatatc atactttcta gctaattctt ggggtttatt
    10981 ttcaccaaca gaagtgattc cttccctcat tgcctcatta actaagtcta catcaacagt
    11041 ctttgtgact gccaaaaggg taatctcttt gctgtctcta tctacttttt ttgcagcttt
    11101 atcaattttg tttcttatct cagacaaatt atctttaata gaacccatta ttatcaccta
    11161 ctttatcctt atatttgtgt ttatactgtt aggtcttaaa attattttgt catatagatt
    11221 aacacttgta actccattta tgtcattttt ataataatcc acaaatacag aagattcatt
    11281 ctcataagtt attccttcta atatgacaaa ttttacactt tgtgattgtt catcaattac
    11341 atatacacca cgtttattgt cttttgtttt tatagaagtt ttaggtattt ttaaacattc
    11401 catctgatta tatattatat caaattcttc gacacttgta tcctgatttg caacaatttg
    11461 gttacttatt tttaatttta ccattatttt attattattt tcatatattt tgtaaacttt
    11521 tgcatctagt ttttgctcat gatattcaat tattacattt tgattttttt caaagttatt
    11581 agaatcttct ttactgatgt atgtagatag ataattactt tggtcattgg catctttttc
    11641 tatagttcct tttacatgaa tctttgcatc aactttttca ttttttaaca ccaacgtatc
    11701 tacatttttg ctaattatca ttactaatat attaaatact aaatatataa gaataccaag
    11761 tatcactaat ctggtatatt ttaccttttt caaagactct ccccctataa aattactttc
    11821 attataagta cttcttctaa aaaatgttta ttcctttttt ataagataat attttacgtt
    11881 ctaataatta ttttttccat attaaattta ttttttatca atcttcctat acaagactgc
    11941 attatttata tataattgta ttataaagta taagtttaat ttacatttta tacgttaagc
    12001 ttactttata tatttttata gttattttta tttttagatg caatataaaa ccaattaaga
    12061 tttaaaataa cttttgttat gttgacattt atttaggtaa tcatttatga attaggataa
    12121 cgttatcaca acaaaagttt atgtcctaaa aaaaatacaa ggaggatttt cttatgtcag
    12181 aaattttatt aggaacaggt ttacttttat tggttctatg ttgtttcaca atttttagtt
    12241 acaaggcccc atatggtatg aaggctatgg gagctctagc aaacgctgca tgtgctactt
    12301 tcttagtaga ggcattcaac ctttctttac ttggtgatgt tttaggtatg gaatttttta
    12361 gaagtgttgg agctgctaat ggtagtcttg gtggagttgc tgctgctata cttgtgcctt
    12421 tagccttagg tgtatctcca gtttatgcag ttttaatagg attatcttgt ttagattttt
    12481 ctatattatc tggattcgtt gctggatact ttgtttcttt cttaattaag tttatagaga
    12541 aaaaagtgcc acctggagtg gatttagtgg ttgtaattct tctatgtgca ccactatctc
    12601 gtcttatagc atcaaatgta gacccattag ttaatagtac actactacaa attgggcaag
    12661 ttttgataga agctaccaac attagcccta taataatggg aattatacta ggaggtctaa
    12721 taacagtagt tggaacgtct ccacttagtt caatggctct tacatctata attggtctta
    12781 ctggtcttcc tatggcaata ggagcattag gtgcttttgg ttcagctttt atgaactcaa
    12841 cactatttgc aaaattgaaa ttaggttcta aaaaagatat tatatctgtt gctattgaac
    12901 cattaaccca atcagacata atttcagcta atccaatacc tatatattct actaatttct
    12961 ttggaggagc cttttctggt atcattgtag ccttgatggg tcttgtaaac atgacaccag
    13021 gtacagctac acctgtggct ggttttgcta taatgtttgc ttataatcca gttcaacaag
    13081 ttcttataac agctggttta tgtgctttat tcaatgtaat tgcaggattt ttaggttcaa
    13141 aaatattcag tacctttaaa gttcttactg ctggtgaaat acgtggtact gatgtagaag
    13201 aagaaattgc ttaaaaaaca ataagattta taaatttata tctcaagtag tagagtttta
    13261 tattgtaggc aattttatta attgcaaacg ttttattttt tatttatgtt tatgattatt
    13321 atgtgtgttg aatatttaga taaattttta tttattattt ttatttagat ttttgtttag
    13381 ttttttattt ggttgatata ttaggaataa aaattagtat agtttattac tagttagatg
    13441 gcataacttt ttagatacat aaaaaatttt atattaatta gatattatta aaataaaagc
    13501 gatacatgaa tttttaaatg tccatgtatc gctttatttt tgccataatc ttaataactg
    13561 atttttaaag tttattatct taacaaagtt cattatctta tataatattt aaatgcatag
    13621 gcttctttaa tttaaccatc taaatattta attaaatatt attatatgta atataactaa
    13681 tttttaaatg aatgtatagg agctggaatc cttcctcctc tttgagtaaa taattcagat
    13741 gaatttgtat ttataggcat tactggtgct cgtcctaaaa gtcctccaaa ttctaccata
    13801 tcacctacat cacatcctgg ggctggtata attctaacgg ctgtagtctt attattaatc
    13861 atacctattg ctgcctcatc agctatcatg gctccaagcg tacctgctgt agtatttcct
    13921 ggcacagcta tcatatcaag acctacagaa caaatagcag tcattgcttc aagcttatct
    13981 atacttaaag cacctttaat aacagcatca atcatacctg catcttcaga tactggtata
    14041 aacgctccac taagtcctcc aacatgtgaa catgccatta ctccaccttt tttaacagca
    14101 tcatttaaaa gtgctaatgc tgctgtagtc ccatgagtac caacaacttc aagacccatt
    14161 tcctctaata tatttgctac tgaatcacca atagcaggtg taggagctag tgataagtct
    14221 attatcccaa atggaacatc caatctacta gaagcttcct ttgccacaag ttgtccaact
    14281 cttgtaattt taaatgcagt ctttttaact gtttctgaaa caacatcaaa aggctctccc
    14341 tttactttct ctaaagctct cttaacaaca ccagggccac ttacaccaac atttataatt
    14401 ctatcagcct caccaacacc atggaaagct cctgccataa atggattatc ttctactgca
    14461 ttacaaaata ctactaattt tgcacaacca aaaccttttg catcttttgt taattctgca
    14521 gtttctttta tgatttctcc catttctttc actgcatcca tatttatacc acatcttgta
    14581 gaacctacat ttactgaagc acatacttta ttagtacttg ctagagcttt tggaattgac
    14641 ttaattagta tcttatcacc ttttgtatat cccttttgaa ctaatgctga gaatccacca
    14701 ataaaatcta tacctaaatc ctctgcagcc ttatccaatg tctgtgcaaa ctttacatag
    14761 tcattttcat ctgttgcacc tgcaataatg gaaattggag ttactgatac ccttttattt
    14821 acaattggga taccaaattc acgctctata tcttttccaa ccttcaccaa atctttagca
    14881 gaactcataa tcttattgta tattttttct ctagctttat ctccatcagg gtctatacaa
    14941 tctaataaag aaatccccat agttatagtt cttatatcca actgttcttc atctatcatc
    15001 tttattgttt caagtatatt tcctacattc actattatca cctcccctta ctgtctacta
    15061 cagattatgc attgaattaa atatttcttc tcgctgaatt tttacatcca ctgataattt
    15121 cttacctctt tcaaccaagt cctcaaccgt tttctcaaaa gaatttaatg atttctctaa
    15181 atcaactatc ataatcatag taaaataatc ctgtaatatt ttttgtgata tatctactat
    15241 gttgatattt tgcttgtata attcatcact aattcctgcc actatacctg gtttatcctt
    15301 tccaattact gttaaaattg ctttcataat aatatcctcc cttatttttt tataaatctt
    15361 atatttttac aaattgaact atgttttaac atatatttca tttatttgtt agtaaaccac
    15421 tttttatgta tatatttaat atattttaat acctatacat tcatttgtca attttttttc
    15481 aatattctat tatttttata ctatttagaa aattcatata ttatatatgt tttctcaaca
    15541 ataaatacag ttttcataat aatgaaaggt attacttagc aatcgcatga ttttctaaat
    15601 aatacctttc taaaaattat ttttttaatt ttatgaattt aattatactt tttctagtat
    15661 caggatttac aattaatata gaaccaatca taattggtgt tagtatacaa aataaaaaaa
    15721 taatatcttt ttttagatta tattctttag attctaattt ataattatta tttattattc
    15781 catcataatc acttatattt gacttatttt tatttgtatc ttgatttttg ttagaaatac
    15841 tttccctata aggaaaagaa tgttcattgg taccaaatac taattttgag cttaaatcat
    15901 aattatcatc attttctgta attgaatttt tattgtaaaa tcctttttct ttatcagaat
    15961 tattttcttt attaccgtct ttaggtatat catgaggatt tttattttca ttgctatcag
    16021 tacttgattc tacatcatct ttaggagtat ttggttgttt ctcatcagaa ttatcatttt
    16081 cattattatt attttcatta ttattgtttt gattgttatt actatcatta ttttcatttc
    16141 cattattccc atcttgattg ttatcatcct catctctatc cgggtcgtct ctacctcctc
    16201 ctccacggtc accatcaata tcatcatttt cccgttcatc atctggtgtc tcttggttgt
    16261 ctccgcctcc cattataaat cctggtttaa aaactatatt tgaataccct tcttttaatc
    16321 ctcctaccac ttttactcta aaacaacgca acttaaaatc ttcaaaaaaa agtatagctt
    16381 gatctctaat atcctcctgt tcttcagata tccaattgat tccatcgctt gaatattcaa
    16441 caaaaacctt acttgcacta tacggcatat cataaaataa aacagctaca tatccatttt
    16501 ttgtattttg aaattccagt tccaaattgt ctattggttt tttctctata acagatacat
    16561 tcaaaatagg aactacatca tcatttatta taattttttc tttatttaac atttctgttt
    16621 taaactttcc tgttatatta aaattttctc ccttttccaa cttttcataa taatcagtaa
    16681 aataccattc tacatctata tcaagtcgat taccactaga tgtttctaat accatatatt
    16741 tgggaaattt tatattttct attttatcat ctttacttac ttttatttca tcactaaata
    16801 caccaattcc cctatgacaa catttcacaa tatcatagcc agaatcttca gctatagagg
    16861 gtataagctc attataatca tcaattatat aaactttctt actttctgat ataattgatt
    16921 gtgccaactt attattatct tttagtaacc cactttcatt gttgctacta tatgctttta
    16981 ttgaagtttc ttctatctca acatcacctt tagaataaac acctatagca tgttctccat
    17041 tcacttcaat atccttattt agtattctaa tatcattttc agtataaatt cctattgcat
    17101 ctttcccatt tgcctttatt ttttcaacag attcgtagga tgatttaata tgtaagtctc
    17161 ctccttcttc aacgtataag gctgtcccat cagtagcaat tatgttgttt ataccaatac
    17221 ttatacttcc accattttct atatgtatta ctccttcttt tcctccttca cctattatat
    17281 tcaattcatt atcatcaata ataaatctcc catgattttt tatcaatatt ttatatttgt
    17341 tggtatctat tgtaaaatta ctgtcataag gaatataaaa tctaaactct tcatctatta
    17401 ccatatcaca agaaagagta taaactattt cttctttatt atttttattt tcttgaaccc
    17461 acctttcaaa atcttcaaga caattttcct cttgtccaaa aactttatgt ggaaaactta
    17521 taaaacacaa aactattata ctcataaaaa taaatatctc atactttttc atcttacact
    17581 cctagaaatt ttttagcttt gaaacaatct gtcgaaacat acacttcacc cactataatt
    17641 tttccacaat tatcaattat tgtcaattta taaaagtatc tttattaatt gttataaata
    17701 aaaagctact tactaaagta aacagacgtt tcactttaat aagtagctat atttttataa
    17761 caaaatagtt ataatattag aacttccttc atttacaatt ttttgtaaag tcttttgcat
    17821 tttaactctt atctcttctg gcatagtata taatttactt tgtaattgct cttttactaa
    17881 gtcatgtagt gatttaccaa acatatttga ttcccataaa tctgctggtt gctcttcaaa
    17941 tacttccata agatatttta tcatctcttc accttgattt tgatttccaa ctattggaga
    18001 aacctctgta gatatatctg cttttataat atgaagtgat ggtgcattgg cttttaactt
    18061 gataccatat tgttttcctt gtttcattat ttctggttct tccagactaa gttcttctaa
    18121 ggaaggagct actactccat atccttttat cttggcatct attaaagcac tttctatttt
    18181 atcatactca tttttaactt tagatagtct agttatcaga ctaagcagtt gataatctcc
    18241 ttcaatttta aatccacttt tttcttcaag gacattatag aataaatctt gctttgtctg
    18301 taaatcaata tttataacac cttctcctag ctctacatta tcaacacctg tatcttccaa
    18361 aaactctaac tcagaaaatc cttgtataat accttctata tcccttattt tacctatatc
    18421 tataatactt tgttttaatg ttgttattat gctacttttt atccaatgat ttctttcaag
    18481 accttcaacc cattttggta agttgattct tatttctgta agtggaaaat catataatac
    18541 agcttccata acctcctcta tatcttcttc ttccatttca accacattca ttggaagaac
    18601 tggaacttca tacttttctt ccaattcact tctaagcata ctcgtttcct cactttttgg
    18661 acttaacgta tttaaaacca ctgcaaatgg ttttttaaga tttttcaatt cttgtataac
    18721 tctctcttct ggttctacat agctttttct gtcaattcca gtaactgaac catctgtcaa
    18781 gacaactatc cctattgtag aatggtcttt tataactttt ttagttccta tctctgctgc
    18841 cttttcaaat gtcatagctt cttgtgacca aggagtggat actaatcttt gttttccacc
    18901 ttcctcatgc cctaaagctc cttcaactat gtatccaaca caatctacca ttcttacttt
    18961 taaagatact gtgtctttta tttttatttc tacaccatct gctggtacaa attttggttc
    19021 tactgtcatt atcgttttac ctgaaccact ttggggtatc tcatctcttg ttctatcctt
    19081 tttaaattca ttgtctatat taggtataac caacttttcc ataaattttc ttataaaagt
    19141 tgattttcct gttcttacag gtccaactac tccaatatat atatccccct gagtcctttt
    19201 ggatatatct tcgtatatgt tattattcat attattcctc ctatacatct aatcttatta
    19261 aattatattt agagttaagg gttaatattc ttaaaataga aaaataaaga ccagcaatat
    19321 taatttaatt tatatataga aatttgtaaa tttcaatgta aattaaatta atattgctgg
    19381 tccaaatagt agtgtattat attaactgac tttagtagta ttttcatttg aattattatt
    19441 catctcaata agttctttac gtctttcttt tgtcatgttc atccatataa atgtaaacac
    19501 caatccaatt atggctacta tagtaagaac tatgaagtag tacctatatc cagttacacc
    19561 cggaaatgca tccaatatct tccctgctgc taaaggacaa agtacttctg gaagatatcc
    19621 taacgtagca actattccta tagcagttcc tgatattgat aatgaataat taccttcttc
    19681 aagtaacgaa tagtgtaaac cttgaattgc atacatagat acatatattg caacacatcc
    19741 aatcaatagt atataaatca tagatgattt tgttggtgta aatattacac ctaaaagacc
    19801 tgcaatcata agtataaatc cataagatat aatctttgat gaaccaactt tatctccaag
    19861 taatccagaa gcagctgatg ctactggtct acaatactga gctaatattg agattgcaga
    19921 tgcaaacacc acagtagctc caaatgcttc agttgaataa ggcgtaaaat aataaaatga
    19981 catattagct gaatatgaac aaaacattat tattgttatt atccatgtat gaggcatttt
    20041 tattactgca aaaaaggctt ctttatcaaa ttttgctgat acctcttttt cattatcctt
    20101 taatttgaaa aatactaata ctccaactat tacatttatt gctgagtata atactataat
    20161 agctgtaaga ccagattcat cacttacttt tgatgaaata tacccaaata gtatcaatac
    20221 tagagataga tgaattgcat tggtaattcc acgaccactt tccataaatc caaaagcttt
    20281 tccttgttca ttttcagatg ctaacattct aacagctttt acaagagctg gccaaaaagt
    20341 aagtattgtt gttattcccc ataaggcatg gattaaaaac acaacaatat atggaggata
    20401 aagtgttaat acaagaccag atattcctgt aataattaaa gaacctgaaa gcaattttct
    20461 ggctgaaata cggtctgcta aaaaaccacc aaaaaggtat gataccattg caagtagacc
    20521 ataaatgctt cctaagcttc ccatttgtgt atttgtaaga ttaaatgctt gtgcaaaagt
    20581 atcataataa taattcttta aatatggtaa agcatatata aatgctcctg ctacaaagag
    20641 tattatcaag actttgaaat ttttccaaat gcttggttta tttacttcaa ctgacatact
    20701 tcctccttat gtaaatacct gtaaattttc ataataatta attattgctt attttattgt
    20761 ttattcaata tttaggaata tttattaata tattaggtta atctatcaat aatttatggt
    20821 gagttatgag ctaaaagtaa actcttttag ctcaccataa taattatatt tttatttata
    20881 ctaacatcca gagttattag atatcttttt ttcatagttc ttccatagca ttaaacctgt
    20941 tattataaca aaaagacctc ctcctatcat ttcaatctca tagattatat tatcttttaa
    21001 agtctccata actggtgccg ttgagaaaag tatgccaact acagttacta caagtaccat
    21061 tctggcaata ttgatacaaa ctgaatcact ttttgcaatt gaatatggtc tttcttcatt
    21121 tggtcttgtc tttctaagtc taatataaga tgtaaaaagc aagacatatg gaaataatgc
    21181 tgttaatgct gtcatagtaa caagaacatt gtatatatta tctactgatg gtaataagtt
    21241 tgttcctaca agaagtagac tcactattat agcctgaact attactgcat ttgatggtat
    21301 attgtattta tttgtaacag tcaattgctt aggaaataca ccttctctta catttccaaa
    21361 aagcatcttt actggacctg ctatataaag tattaatgct cctagtactg ataaagttat
    21421 tccaaatgct actattctta taaaccaact tcctatacct aaatcttgtg caactttagc
    21481 taatgcatct agtatccctg tagatgctgc aattttatct ggaggaagta tcattgttat
    21541 tgctactgaa cctaagatat agattcctcc tattaaaaca gccacagtta atatagcttt
    21601 aggaaagttt ttcttagcat tatccatttc ggttatgaaa ttagcagtcg tttcagctcc
    21661 tgataatgca aatattatag cagatataga aactaatgaa ttggcatcta tttttggtat
    21721 tatattttgg attgtatata tcgatgctga tggagctttt ttgagaataa ctacagacat
    21781 aaatgccatc actataagta atatagcagg aattgtagaa cctaatgctc ctgtattcgt
    21841 aaaaaatttt ccaaatgcca tcccacgagt acttactaag gacaacagcc aaaatattat
    21901 gagtgaaagt gctaaaataa acatcttatt gtttgataaa tctggatttc caagcatata
    21961 tgcaatattt atcgctaaaa aagtcaagaa agaagaatac caaaatatct ttgatgtcca
    22021 atttagccat gacaccataa atccccactt ctctccataa gcttgtttaa cccattctcc
    22081 aagtccacca tcttttggat atgttgcagc caactctgca catataagtg atgcaggaac
    22141 aaagaatata aaagcaaaca ataaccaaac tggtatagaa ccaagaccca atccaaaact
    22201 agaggcagta gatttagcta tccatctaat cccaaatagt gctgatatat tcataaatac
    22261 taaatcccaa aatccaattt tctttttcac tgtaattcct ccaatttcaa atttattcag
    22321 actcatacac aatgtattga cttaaatatc aagtataagt tcttcaacac ttcttggata
    22381 attagttata attgtagcac cattcttacc tactattgct gtatctgagt gtctaaatcc
    22441 tccaactcca ggtatatata tccctggctc catagaaaat accattcctt cttctaatat
    22501 taattcattg tcaaatctta aatatggctc ttcatgttct gataaaccta aaccatgacc
    22561 aattctatga tttgaatata actctagacc atactttgca acaacagctc ttgctgcttc
    22621 atccaccatt cttgctggta taccagcttt tattgtatct aatccagctt gttgagcctc
    22681 tactgcaatc ttaaacactt ctttttgtct ttctgttggt ttgcctataa taaaagttct
    22741 ttcattttct gctctatagt tttcatacca aacctgtcta ctatgtatta ctatatctcc
    22801 tctttgaagt attctagtat tggaatctaa atgaggttgt gctgttctat caatccctga
    22861 acaagtccag tttgcaaatc ctatatatgt atcaggataa ttttcagaaa catattttag
    22921 taaagcatta tcccctgcaa cgtcaaactc caattcactc atcccaactc taacattttc
    22981 taaagaccct tttattccta tatcagatag gtaacctgct atcttcaaaa attctatttc
    23041 ttttgcatct tttacatatc tcatttcaaa tattttactt ccaacatctt tgatagcaaa
    23101 tgacttatca gttatatact ctgaaaagct tgcagatact atatcttttt ctattccaac
    23161 agttgttcct ttagggtatt tatttaaaat tatatctaag taatgtttat gtgatattcc
    23221 atgttctttc atttctggta tttcataata cacatataca ttatcaactt ttgcaacttc
    23281 tttagcatgt aattcttcta atccaggtac tattaattct attttatctt gagttactac
    23341 tgttacaatt ggtctagaat aagttattgc tttaaatcca ctaatataaa attgattgtc
    23401 tggttcaaat attaaagcaa tttcaatgct tttttcattc atgtactttc tcaatctttc
    23461 tagtctcata ttatttgaca taatgcacct ctttcttaat attcagataa tttattcaat
    23521 acaacaagga tgaatatatt attttaaaat tcttaatatt tttagtactt gtcgtatttg
    23581 tatgtaaatt aattttattt gtgattattt tatcataatc atgattattt tgtcaa
//
LOCUS       DAYASV010000053        23137 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYASV010000053 DAYASV010000000
VERSION     DAYASV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23137
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.053"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            107..1339
                     /locus_tag="ACGI68_002003"
     CDS             107..1339
                     /locus_tag="ACGI68_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFH0138441.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMNCFGIKDK"
     gene            1353..2159
                     /locus_tag="ACGI68_002004"
     CDS             1353..2159
                     /locus_tag="ACGI68_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HFH0138442.1"
                     /translation="MGRKVKIGIIQQHSVLGNVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2271..3140)
                     /locus_tag="ACGI68_002005"
     CDS             complement(2271..3140)
                     /locus_tag="ACGI68_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0138443.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3512..4939
                     /locus_tag="ACGI68_002006"
     CDS             3512..4939
                     /locus_tag="ACGI68_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HFH0138444.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVEIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5016..5285)
                     /locus_tag="ACGI68_002007"
     CDS             complement(5016..5285)
                     /locus_tag="ACGI68_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138445.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMKGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5288..6730)
                     /gene="nhaC"
                     /locus_tag="ACGI68_002008"
     CDS             complement(5288..6730)
                     /gene="nhaC"
                     /locus_tag="ACGI68_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFH0138446.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6755..7921)
                     /locus_tag="ACGI68_002009"
     CDS             complement(6755..7921)
                     /locus_tag="ACGI68_002009"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0138447.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8146..10266)
                     /locus_tag="ACGI68_002010"
     CDS             complement(8146..10266)
                     /locus_tag="ACGI68_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138448.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSDNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQNFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10499..10900
                     /locus_tag="ACGI68_002011"
     CDS             10499..10900
                     /locus_tag="ACGI68_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138449.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQVGK"
     gene            10943..11236
                     /locus_tag="ACGI68_002012"
     CDS             10943..11236
                     /locus_tag="ACGI68_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFH0138450.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11251..11691
                     /locus_tag="ACGI68_002013"
     CDS             11251..11691
                     /locus_tag="ACGI68_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HFH0138451.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11783..12256)
                     /locus_tag="ACGI68_002014"
     CDS             complement(11783..12256)
                     /locus_tag="ACGI68_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0138452.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12256..13044)
                     /locus_tag="ACGI68_002015"
     CDS             complement(12256..13044)
                     /locus_tag="ACGI68_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFH0138453.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13372..14616
                     /gene="glyA"
                     /locus_tag="ACGI68_002016"
     CDS             13372..14616
                     /gene="glyA"
                     /locus_tag="ACGI68_002016"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFH0138454.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALITSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            14939..16036
                     /locus_tag="ACGI68_002017"
     CDS             14939..16036
                     /locus_tag="ACGI68_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0138455.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16027..16725
                     /locus_tag="ACGI68_002018"
     CDS             16027..16725
                     /locus_tag="ACGI68_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0138456.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16728..17792
                     /locus_tag="ACGI68_002019"
     CDS             16728..17792
                     /locus_tag="ACGI68_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFH0138457.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            17975..18679
                     /locus_tag="ACGI68_002020"
     CDS             17975..18679
                     /locus_tag="ACGI68_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138458.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18672..19994
                     /locus_tag="ACGI68_002021"
     CDS             18672..19994
                     /locus_tag="ACGI68_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0138459.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20181..21554
                     /locus_tag="ACGI68_002022"
     CDS             20181..21554
                     /locus_tag="ACGI68_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138460.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYIFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(21926..22864)
                     /gene="pdaA"
                     /locus_tag="ACGI68_002023"
     CDS             complement(21926..22864)
                     /gene="pdaA"
                     /locus_tag="ACGI68_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFH0138461.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8517 a         2882 c         3668 g         8070 t
ORIGIN      
        1 tttgtagaat agaattacaa ttatataaag aaaaattcta ttaatagtga aaaaaaagta
       61 gaaaaaagta gaaattattc ttttataaag aaagggaggg ataatattgc aagagagaga
      121 agaattagtt ttaaatccag ttccaaaaga ggaaagagtt ggatggttag ctccattgtt
      181 taatatgctt ggttctaaca tagctatatc tgaactaatg gttgggggaa ctttaatttt
      241 aggaatgact ctttctaata tgattattac atcaattata gggaacttaa tattagttgc
      301 aattattatg atacaaggtt atataggtta caaagaaggg ttaaatacgt atgtattagc
      361 taagggggca tttggagaga taggaggaaa gtatcttata tctctattac ttggtataac
      421 tagttttgga tggtttggtg tacaagcagg ggttgcagga ctatcagtac aaaagatatt
      481 cccaagtgta aacctaacat tagttacagt tatacttgga ttatttatgg ttgtttttgc
      541 actgtatggt tttaaagcta tggctaaatt taattactta gttataccac cacttatgat
      601 attaatggtg tggggagttt ttaaagcttt ttctacttat ggagttgaag ctatttataa
      661 ttatactcct caaacaacta tgagtatggt tgaaggtctt aatattgtag taggtcttgt
      721 tatagttggt gcaattatat cacctgacca gttaagatat acgagacgag taaaagacat
      781 ttggattatc agttttttag gtctagggat tatttcatta ttccaacaag ttgcagctgg
      841 tgtaatgtct atgggagcac ctacttggga tataacagaa gtattggcta atttaggttt
      901 tggatgggta gcgtttgtta ttttaatact tgcatcttgg tctacaaacg tatcaaatgc
      961 ttattctggt ggattagcat taaaaactat ttttccaaat gtaaaaagaa atgttttgac
     1021 tttagttgca ggtctaatag gtacaataat agcagctact ggtataatat ttaaatttca
     1081 gtcattttta tctttcttgg gaatcgcagt tcctgcaata gcaggaatta tgtggtgtga
     1141 gtattatttt atacaaggaa gaacttataa gcatagagaa ggaataaatt ggattgcagt
     1201 aataagttgg ttaataggtt ttgcagcgtc gtattatagt tcaaaaataa atttcttaat
     1261 acctccgata aatggaattg tagtgagtat ggttatttat tatatattaa tgaattgttt
     1321 tggaatcaaa gataaataaa gggggcaatt acatgggtcg taaggtgaaa ataggtataa
     1381 ttcaacaaca cagtgtactt ggaaatgtta aaaaaaatat agaaaaagca gtagagatga
     1441 tagatgactt aggaaaacaa ggggcagata taatttgttt accagaacta tttgctacag
     1501 gatataattt agagtcttta ggtggagtta aaacattaga attaataaga gaacataata
     1561 agtacataga ggaatcaatg tcagaagctg ctaaaagaaa taatgtatac ctgatatctc
     1621 catatggaac cttagaaaaa gggtctacac atgtttataa ttcagcagtt atatttgata
     1681 gaaaaggtaa gattatggga gaatactgta aaaatcatct gtggtcatta gaagcagtat
     1741 actttaaagg aggggaaaaa gtagaggtat atgatgctga ttttggtaga tttggtgtaa
     1801 tgatatgtta tgatgcaggt tttccagaag tatccagaga attgacatta aaaggttctg
     1861 aaataatatt tataccatct gcatggagaa tacaagatga agatatgtgg gatttaaatg
     1921 tatctcaaag agctttagag aacactgtat atacagtagg tgtaaattta gtaagtaatg
     1981 actctaattt aatactattt ggaaagagta aaatatgcaa tcctagaggt actgtaataa
     2041 ctcaattaga tacatacagg gaaaaatatg tattatcaga gattgattta gatgaaataa
     2101 aaaagtatag ggaagatatc ccctatttga aagacagaaa aagtagctat aacatataat
     2161 tctatactat aatacaagtt caatataaaa tgataaaaag aggatgtatc aatattaaga
     2221 ttaaatttat tatactttga tatatcctct ttaatattat caactttgat ttaaatattt
     2281 ttaaaatagt tgataagaaa gtttttgaac aacaacacag ctttagattc atagttttcg
     2341 ctaaaccatg aaagatttaa atatctttta aattcaatat ctttgatatg taacaactta
     2401 atctcagggt cttgagataa tccccatgat tttccacata taaatccaac tccttgaagt
     2461 gattttataa gagcataaat tgtatatggt gaatcagact caaatgctat tttgggtttg
     2521 aaattagctg attcgcacag gatatcaatt acctctctat aattctcacc cttcgttata
     2581 actataaagt tttcatctga aacttcgctt agatatactt catcctttaa tgataaagga
     2641 tggtttattg aaactcctaa tagtatttct tcgcatgtta aagttatgct attttctgag
     2701 tttagctttg taaatgaaga agatatgtac aaatcaaaat cacttttttt ataagatgat
     2761 ggcaaagtat gtgacacatt aaaagtaata ttgggatata gttttctgaa attactcaaa
     2821 agtgatggta acacattagc cgctgcagga gataatatct ttacttctcc tgaattttcc
     2881 aaagaagtgt cttgtaactc cttttttgaa ctttctacta aatctaaaat gctatctact
     2941 ctttttagaa acaatcttcc atactcattt aaaacaatat atcttccttt tctgtcaaat
     3001 aactgaactc caagttcttt ttctaaggaa gatatagttt tgcttaaagc tggctgtgct
     3061 atatttaact tgtgtgatgc ttttgttatg tgctccattc tagcaacagt ttgaaaatac
     3121 ttaagatgca ataaatccat agtataaaat acacctcttt taatatgact tgagttatat
     3181 caattttata atcaactcca cattatacat tataatataa agtttatata ataaactaca
     3241 atatattaaa aagttattta aattgttatt tatattttgt acataatcta attaaaaaaa
     3301 gtaactttaa atggttaact aaggaaatat tgttacaaac tttagttata taaaaaattc
     3361 gtttgatgag caaagtatta atgtcaagta agaataaaag cttattagat aagaaaaata
     3421 atattttata ataatgatgt tacttaaatt attatgttat actattttaa ataggaaaca
     3481 tttagatact ataattttag ggggagtcaa gatgaaatta actaaatcaa tatcatcaat
     3541 gctgtgctta tctctagttt taagcttatc accagtagca tatgcaaatg aaataagtag
     3601 aacaacagtt gagaatactt tagtaggtca ggatagatat caaactgctg ttgaagtaag
     3661 taaaaaagga tggagtagtg caaatgaggc tgtagttgta aatagtgaat ctattgtaga
     3721 tgcattagct gtaacaccat ttgctaaatt aaaaaatgct cctatattat tgacagaaaa
     3781 aaataatcta aattcacaaa ctgagaaaga attaaaaaga ttaggagtta aaaaagttta
     3841 tataattggt ctatctagtt ctgtatcaaa tgatgtgcaa aaacagttag aatctaataa
     3901 tttaactact gatagagttg gtggtagtga tagataccaa acagctttga gtatagctaa
     3961 aaaaatagaa ggattaaaag atatatcaga aatagctgta gtaaatgggt acacaggtct
     4021 tgcagatgca gttagtatag cttctgtagc tgctactaat ggaatggtta tacttccagt
     4081 atctgatgca tctggtatat ctagctttaa agattttata gaatctaaga atataaataa
     4141 atcatacata ataggctcta ctaatgctgt atcagataaa ataaaacaaa gtttacctaa
     4201 ttcagaaaga ataggtggag cagatagaaa tgaaactaat ggtaaagtaa tagaaaagtt
     4261 ttatacatct aataaattaa acaatgtatt tgtggctaag gatggaatga aaaatcaaaa
     4321 tcaacttata gacgctttag cagtaggagt actagcatca aaagaaaatt ctcctgtact
     4381 aatcgtagga gaacaattaa gtgcaacaca aaagagtgta ttttcgaata aaaatgctac
     4441 ctctataact caagttggta gtggaggaaa tgaaaatgca tttaaagaat taaaagattt
     4501 acaggcaaat agtaacaata agagagtaat gtatgtaact aatacagata aggtaaatat
     4561 aagaaatgat gctacaatag aagcttctgt tataggatat ctaaataatg gtgatgaagt
     4621 agaagtttta gatgtgttaa aaactggatg ggtagaaata aaatacaatg aaggtattgg
     4681 atatgttagt gggtcttatt tgacaaataa taagcctgat aatagtaatg aaaatataaa
     4741 aataaagtat gtaaaagaaa aagatggtct aaatgttagg aaaggaccaa gtacagaaga
     4801 tgaaaaaata ggtcaccttt catatggaag caaagtagaa acaatagaaa tgtttgctac
     4861 tggatgggta aaaataaagt ataatggtgg atatggttat gtaagtaatg attacttatc
     4921 tgatacacca gttatttaat tataagtaac aataaaaaaa ctgctttaat ttgtaaagca
     4981 gtttttttat aaaaatttaa gtctaataac tatttctatt ttgatacctt aatcctggga
     5041 tttattatta tggatattat caacatacaa acactccaaa tccagtgtct aattccaaac
     5101 ttagatatta ataaattcag tcctgctatg gtaaataagc agtatttaat tacaattaaa
     5161 agtgtattta tagaaagccc cttcatagaa agtatcctaa atccaataat agacattcta
     5221 agtcctataa atgccaataa tatacctgca atatcctgaa ttaagtaata gtattcaaat
     5281 ttcatattca atcaactctt ctctgagcag tatcaaatat ttctaactct tcaatttcat
     5341 catctgtgta tgtaagcata gtaaaaccag ttacaccata aataaatgaa attataggtg
     5401 taatccaact taagaaacaa tatggaataa attctagtgg gcttacacca agagtctgag
     5461 cacagaaaac agcatttgaa ttccatggta ttaatggacc accaagtgta gctgtatctt
     5521 ctataattct agatagattt tgtggtttta atttaaattc tttgtatatt ggagacatta
     5581 gagttccgac aaatacagaa gtgaacatca ttgatgaact aaatgcattt gcaaagtaac
     5641 ttactataaa agtagaccca accaatttag ttcttgattt tcctatttta gttataagag
     5701 gttgaagcag tacttctaag aatccagttt tttggagaat accagcaata acgaaaacta
     5761 caaatactag taaaacggtt tcagccatac tcattattcc accacgattt aatagtttgt
     5821 cggcatagac aaatccagta tctatagtaa atcctgatag catgtaattt aaaacatcac
     5881 ctattttttc tccttcttga aatactgcta taataagtcc catgatagct gaacttagta
     5941 tagatataat gggtggcttt tgtagcaata aaagtaataa taaaaatatt attggaatca
     6001 ttgctactaa accaatatga aagtttgaat ttaatacatc cttgatttga tttatttgaa
     6061 catagtctat agtattattt gaatacttaa atccaattac tgtatacaga actatacata
     6121 ttacgtatgc aggtcctgtg gtataaaaca tatgcttcat atgtgtaatt acatctgtct
     6181 tacaaactgc tgcagctaag tttgtagaat ctgatagagg tgacatctta tctccaaaga
     6241 aagcaccaga aatgactgca ccagctgtta aaccaagagg aatacccata ctagtaccaa
     6301 tactcatcat tgctatacca gaagttccaa gagtccccca agatgtacca gtagcaacag
     6361 aagttaatga acatacgatt aatgttgtaa gcagaaaata tttaggggtt attattgtta
     6421 gtccactata tactactgtt ggtattgtac cagcagctat ccaagctgct attagtacac
     6481 caacagaaag tataattaag tttgattgaa aggcatcttt accaacttca aatccaaaag
     6541 cttctatttc atcattagta taaccaagtt tcatagctgc aggaactact ataagccatg
     6601 aaagtaaaaa cattgtagtt atagaagcat taaatacaac tataccaact gatgttagag
     6661 ctattattgc aagcattact aacaaggaat atctaaatga aggtattttt ctttctgtat
     6721 tcattttcat agtgcaaatt ctccttaaat atttttagtt aattgcattt tttattctta
     6781 taagagcttc ttcaagcata cttcttggac aagctaagtt tattctttga tacccacttc
     6841 ctccaatgcc aaagctgttt ccttgattta gagcaacttt acctttttgt acaagtatac
     6901 tttctagttc ctcatcactt aatccaagtg cactaaagtc tacccataat aaataagtac
     6961 cttctggttt tctaacttta agttttggca tattttcatt tatatatttt atagcaaaat
     7021 ctatattaga ttctaaatat tctaaaaatg attctagcca agattcacca ttattatatg
     7081 aagcttcagt tgcaactaag ctaaagcaat tatttctctt tatatcaatt ctggtaaagg
     7141 catcatctaa caatttatag tctttttcat caggtagtat tacatatgat gattgaagac
     7201 cagctatatt aaaagtttta gttggagcca tacaagtaat tgtatttttt tcaaattctt
     7261 tagaaataga tgccatagga atatgcttat gtttttttaa tataatgtca ctatgtattt
     7321 catcagatat gatttttaca ttatgcttaa gacaaatatc tcctagtttt tttaattcat
     7381 cttttgtcca tactctacca actggattat gaggattaca aagtatgaat aattttacat
     7441 ctttaatctt gttttcaatg tcttcataat ccattatgta gtttccattt tctagttttt
     7501 gtaatgggct tataattagt tctctattgt tattttttac aacactatta aaagggctgt
     7561 aaactggttc ttgtatcatt attttatcat ttgcttttgt gagttcattt attaaaagac
     7621 ttatagctgg gataacacct ggactataaa ttaaccactc actttttatt ttccagttat
     7681 gtctcctatc tagccagttt actatagatt cattgtaaga atctggtctt gttgtgtagc
     7741 cataaatctc ttgttctagt ctatttttta aggaatcaat tatacaaggt gcagccttaa
     7801 aatccatgtc agccacccac ataggaagta aatcattagt tccatacttt ttttccattt
     7861 ctgaccattt agatgaaaaa ttattgcttc tatcaacaat ctcattaaaa ttataattca
     7921 ttgtaaatac ctccagtaat tttaatttta tgttaataat attaagcaaa caacgtgcca
     7981 atattgataa taaaaaatat ttattcacaa gttaatattt aatattaagt aatttcagag
     8041 catagataag ataaaaatat taattatact taaaactttg tggaaaaggt tatttctaat
     8101 aaatttttaa atatatgaaa atgtaattaa ataggttaaa taggtttaac tgtttaactt
     8161 gttagaccta tttatatttt ttagaaattc tactcctttt gatgttataa ttgaacctcc
     8221 acgaccaaca agtatgtcta caagcccaaa gttttgtagt tctaaaagca tatttctaat
     8281 ctgttgttct gtcaaaaatc tattttcctc taaagctatt ttacataagc tttttctacc
     8341 tattctttct tttaaatcat aagccttttt taaattatcc aatataaata tgtattcatc
     8401 tagtgacctt ttaaaattat agtcgtattt catgatgttt ttgtctttgc caatgttgaa
     8461 ttgatacttt tttatattat cacttacttt attacaaacg gttctactat aatttgaatc
     8521 tatagtatct aatatatatt caggtaaatc acatatttct ataatatctt tttccaagta
     8581 acagaagtat tctcctaagt ttcttaattc tcttacattt ccttcccatc tatacatttt
     8641 aaagatttct tttacttcct cacttagaat aaaattacat ggaatatcat attttaatga
     8701 accaaaaatt tcaaatatat cagcaggtct ttccctaagt ggatgtattt taaggggaag
     8761 aacattcaac ctaaaatata aatctcttct aaacatattt ttagatacaa gttcttttaa
     8821 atttctatta gtagctgcaa ttattcttac atcaatgtca atgacattat tagaaccaat
     8881 acgtcgtact tgtttttctt gtatgactct aagtaattta gattgagaat ttaaatccat
     8941 ttctccaatt tcgtctaaaa atatagttcc attgttggct aattcaaata aaccaatttt
     9001 cccacctttt ttagcaccag taaaagcccc ttcatcatag ccaaataatt cactttgcag
     9061 tagattttct tgaaaagtac tacaatttac tgcgacaaaa ggaccatctt ttctcctaga
     9121 agcactatga atggattgag caaataattc tttaccagta ccactttcac caattataag
     9181 gatagatgaa tttgattgag ccattttatt agcaattttt tttgtattaa tagtttgtat
     9241 actagaacct aatatgtcat caaatgtgta tttactgata ttaccagagt ttactagttg
     9301 tgctcttaat ttagcttgct ttttttctaa ttgagaaaac tttgtcatct ttagtataaa
     9361 tccatcaaat acatttaatt gtatatgttt aatctctaaa tttatatcaa tatgatttat
     9421 tttaatcaat ttctcaaaaa atggagtttt attggtcaat atgctttgaa aattgatgtc
     9481 ttttatgcaa tcaccaacaa atgagtcaat catttcattt gcatttatag acaaaatttt
     9541 tctagccata tgattataga attgaattat tccatcgtta tttgtgcata taattccatc
     9601 atcaattata gatagcaaca aatcaaattg tctttcaaac ttatttgcat ctaacaataa
     9661 ctcacctgta ctataacttg taggaattat tttttctacg tattttttta ttaggtcatc
     9721 ttttattaga tgttttaatt tggtttttgt agcaatttca acaatacaac ttaaatctat
     9781 tattctgtat tttaaatcta taacttcaca atttttaaac ttaggcaaga ttttttcgcc
     9841 aggagttagg attacagcat ctgtgggagt aaactcaatc tctggatagc agggcaggat
     9901 atttatattg tctattccta atctatgtat taaagaaatt gtttctattg ccatatcttt
     9961 acttaagtta tatacataaa taattttatc tttttctaat ttggatatca tatcaatact
    10021 gttcttttca aatgtcaact taggaacaat gatttgagca tcatagtgag ataattttac
    10081 tatttcatca tattttaaat aagcagatag gagtataagt ttttctttaa aaaactttaa
    10141 atttccctct tcaaatgagt ataaatttat ctttagatta tcacctaaga attgccttat
    10201 ttgattttca taaatttctc cagcgtcttt ttttaaactt acaatagcta attccatttg
    10261 tttcatttta acctctcata caaatttgac ttattttaga ttacataata attaaattag
    10321 attaaataat attatttaat tattattata aatttattat agactacaca ttgaagtaaa
    10381 agaagtagct aaaattattc cttttatttt tgttttttgt atctatttgg tataatagta
    10441 ttagagcgta taataattta ggcaaaattt tttaaggaag actagaggag aatataaaat
    10501 gaaaaaatgt ctttactgta atagggatat aaagtcacca aataataaat tagctataaa
    10561 atatgatgac aatatgaata tatatccatg tcgtgcagaa tgtaaggaaa aaattgaaga
    10621 atatatagca aaaaaaccta catataagtt tataaatata ttagaggttc ttttaggtgt
    10681 gggcggaata ttctgctttt tgagtaaatg gactattgca gcccaagttg tacttggaat
    10741 tgatgcactg gttatatttt tattcccatt ggcaggattt aaaatgaatg gaaagttaag
    10801 tatggaacaa actaagaatc aaagtaggtc tattgcaata tcaattttgg catttatagt
    10861 aataataact gtattatatt ttaaacaagt tggtaagtag agcataattg tataagacat
    10921 cattagagta tgaggtgagt ttatggaaga aagaaaatta gcaccaaaca atgagaggga
    10981 agctctcata aaaagattaa aaagaataga aggtcaagtt aagggtatac aaaagatggt
    11041 ggaacaagaa cgttattgtg ttgatatatt ggttcaaata tctgctataa ggtctgctat
    11101 aaatagagta ggcacaatta tcttagagaa tcatataaaa ggatgtgtag cagaaagtat
    11161 taaacatgat gatgctgata aaactgaaga gatgataaaa gagcttatgg atactataaa
    11221 taaatttaca aaataaggag gcggttagtt atgaatcaac aaggatttat tatggaaatt
    11281 gttgatgata gaactgcaaa gttaaaaatg caaagacatt cagcttgtgc tgcttgtgga
    11341 aaatgtgcta ctacaaattc agcagagtca aaagaaatat tagtagaagt agacaacaca
    11401 attggtgcaa aagttggtga ccatgtagaa gtgagtatgg ataatatgaa tgtattaaaa
    11461 gcagctgtaa tggcttatat aatacctctt atagcacttc ttgtaggaac aatagggact
    11521 tattatatat tgggggcaat tggaatgaca actggagttg aagccataag tggattagtt
    11581 ggaatttgtc ttacaataat ctgttattta tacttaaaga agaatgacaa aaaatttaga
    11641 gacagtagag aatttatacc agtaataaca agaatattaa ttgaccttta agataattat
    11701 aattaaaaaa tagcaatgta ctttttaaat aaatactcaa tacgtatttg tagagtttaa
    11761 gtacattgct atttttatat ttttattttg attttctgta aggaataaag ttttttctga
    11821 tttttaaaag attcaatgtt cttacaaaag aagttacaac taatacacca agtgctattg
    11881 cagcacttag aaatagcgca tctgtacctt ttgacatagc caaagaataa tttttctgaa
    11941 tcaaagacaa cattgtatta tatagcccaa ttcctggtat tagaggaaga atacctggaa
    12001 ttacgaacac aatagctggt tgctttagtt ttcttgctaa agtctcactg cacgctgaga
    12061 ccaaagcagc agcgataaaa cctgcaaaaa ctgcatttgt agtaaaatta aataaataaa
    12121 catatacaat ccaaccaatt ccgccagtta ctccagctgg aagaagtaga tatagtggag
    12181 cattaaagaa aacagcaaat ccagcagttg caacacctga aaataaaaaa tgcaaaatca
    12241 tagacatatc agtcattaga aaggacctcc tattctatac catgtatcaa ggacaaaacc
    12301 aactccagaa gcaattgcaa taataatcat agcagcatca aaagcacgag aaattcctga
    12361 tattaaattc cctgatatta aatctctaat agcttttata aaagcaactc ctgggagtag
    12421 tggcattata gcgccgacta taagcatttt agggtcatct aaaatgccta tttcaactag
    12481 tagaactcct gaaagtgcaa ttaaaaaaga agatactaaa caggaaaatg cagatattgc
    12541 acttatcttc atcgttttat cataagtaat tgcagcaaag attgctatta taaacgtaag
    12601 tataaaattt aatacataat tccctccaag caaacaagca aaacaagcag atgcaattcc
    12661 tgtacaagag taaaataacc ataaaggata aggttttaca tcttggattt cataaagacg
    12721 tgcaatggca ttatcaatat catactcttt gtcactaaca aattctcgag aaaaactgtt
    12781 taataaagaa atcttattta agtttatacc tcttgattga atcgttttca taaacgaaaa
    12841 gccatcaaat ttctcatctg aaataattat tactgtagga gttgtaaaaa tatttacatg
    12901 ataaaaccct cttgacctgc atattctaag gacactgtct tcaaccctag atgtttcagc
    12961 tccatttttt atcatcagtt cccctacaaa tagagctagt tttagaatat ttttcttata
    13021 ttcaagttcc tcattttgat tcataaatac acctccaaat taaaaaacgt aatattgaaa
    13081 ttatatcaaa tttttaagta atattcctta atatctatac aaatatatta tctttttttt
    13141 gtttcaaaat agtcacattt aaattatatc caattaaaag aattagacaa tattatttat
    13201 tatattttaa cttaaaaata aagaaagtaa ctatgttaaa tatggaaaaa tggcaaaaca
    13261 cttgactatt aattggtaaa ggactataat aataaattat catattgata aaaacgaaaa
    13321 ttatgaaatg tctgtaaaaa taaaatttaa ataataacta ggaggagtaa tatgtttgag
    13381 aacttgaaaa aagcggaccc tgagatttat gaaagtatga aaagagagtt aaaaagacaa
    13441 caaagaaata ttgaattaat agcatcagaa aacatagtat cagttccagt aatggaaact
    13501 atgggaagtc atcttacaaa taagtatgca gaaggttact caggcaaaag atattatggt
    13561 ggatgtgaat acatagatga agttgaaact cttgcaatag atagaatcaa aaaaatattt
    13621 ggagcagaac atgctaatgt tcaacctcac tctggagcaa atgcaaatat tggtgtatat
    13681 tttgctatgt taaaaccagg tgatgttgtt atgggaatga atttatctca aggtggacat
    13741 ttaactcatg gtgctccagt taatatttct ggtcaatatt ataaatttta tgaatatggt
    13801 atagataaag acagtggact tatagatttt gatgaagtta gaaagatagc acatgaagta
    13861 aaacctaaaa tgatagttgc aggagcaagt gcatatccta gagaaataga ttttaaaaag
    13921 tttagagaga tagctgatga ggttggagca ttgcttatgg tagatatggc tcatatagca
    13981 ggtcttgtgg ctgctggtct tcaccaaaat ccatgtgaag tggctgattt tgttacaaca
    14041 acaactcaca aaactttaag aggacctcga gggggtgtta ttctttgtaa agaaaaatat
    14101 gctaaagaca tagacaaagc aatattccct ggtattcaag gtggacctct tgaacatata
    14161 attgcttcta aggcagtttg ctttaaagaa gctttaagtg atgagtttaa agaatatcag
    14221 gtacaagttg ctaaaaatgc aaaagcctta gctgaagaac ttattaaaag agattttaaa
    14281 ttgatttctg gaggtacaga taatcattta atactattag atttgactaa taaaaatata
    14341 actggtaaag cagcagaaaa gagattggat gatgcttata taacagctaa taaaaataca
    14401 attccatttg acccaaatgg tgctcttatt acaagtggaa taagacttgg tactccagct
    14461 gtaactacta gaggtatgaa agaagaagat atggcaataa tcgctgaggc tatagaccta
    14521 tgtttaactt atgatgaaga atctaaggct agaacattgg ttgttggttt aactgaaaaa
    14581 tatcctttat atgaagaata taatattatg gattaattat aaagaacaac taaataaaat
    14641 acatttaaat aattacaatg attgtgtatg tatttgaata tagttagata taaaaaaggt
    14701 gtctcaaaat gaacttttta gtttatgagg cacttttttg tatggaatcg aacatatttg
    14761 tgttatttaa ataataaaaa atatggttat ctctattttt agatggtctc tttgtatttt
    14821 ttaattaaaa ctttaagatt tcttaagaat tatactaggt ttttctaaag atttaaagtc
    14881 tatacttata agtatagtaa tcatatgaaa agaaaagctt taacgaggag ggaatcttat
    14941 gaaggtatta attcttacag gaaaatttgg tatgggtcat tactcagcat caaattcttt
    15001 aagtgaagat ataaaagcaa aatttgataa ttcagaaata ataattaaag atatatttga
    15061 gtacattatg cctaactatt cagataaaat gtacaagact ttttctattc ttgtaaatcg
    15121 gggaagtagt ctttataatt tattttataa atgtgctgaa aatggaaaga aagatataaa
    15181 atttactttt tcagattatt ttttaaataa attagataca ttacttcatg aagtacaacc
    15241 aacagtagtt atttcaactt ttccattttg ttctcaatta gtatcaagat ataaggaaaa
    15301 atataactca aatcttcctt taataacatg tataactgat ataagtagtc actcagaatg
    15361 gattagtaaa aatacagact gttatttagt agctagtaaa tctactaagg aagaacttgt
    15421 ttttaaagga atagatgaat ctaagattaa agttaatggt attccagtaa aaaaagaatt
    15481 taaaagaata gaacatgtaa atcattcaac taaaaaaaat attcttataa tgggtggagg
    15541 attaggatta ctgcctaaat cagaacaatt ttacaaagaa ttaaatagtt tagaaggagt
    15601 aaaaactact gtaataactg gtaataataa aaaaatgtac tataaactat atggtagata
    15661 tgaaaatata gaggtagtag gttacaccaa tgaagtatat aaatatatga aagattctga
    15721 ccttattatt tctaaaccag gtggaataac actatttgaa acaatttatt cagaacttcc
    15781 aatattggca tttaatccat ttttacaaca agagatagat aatgctagtt ttatattaaa
    15841 taatgaaatt gggagaatac ttggtaaaaa taaaaaatac tatgtagatg agataaaaga
    15901 tttaatttat gatgatgcta ctcttaaaga aatgagcagt aacatgaaag aattaaaaaa
    15961 acaatttgat aataatacct tagagaatat tttattttca cttgatgaac aaggagcgtg
    16021 tagagaatgt atgtagtagg tttaattatc gttgttgcat tgattttttt agtccattca
    16081 attataccta catactataa taaactatta aataaagagg tattaaaaaa tatggctgga
    16141 gaaaatgaaa ttgcacttac atttgatgat ggtccagata agagatatac agaaaaatta
    16201 ctagatgtgt taaaagaaaa tgatatacaa gctatgtttt ttgtagtagc aaagaatgct
    16261 gaaaaagagc ctgaaattat aaaaagaatg ttgagagaaa atcatatagt tggtttacat
    16321 tcattggaac acagaaatgc atggctttat agttatagtt atgttaaaaa agattttatt
    16381 gaaagtacaa atataatgaa aaatttaggt gtagatgtaa actactatcg tccaccatgg
    16441 ggtcatacta atatattttc aaattcattt gttaaaaaat ataatctaaa aatgactttg
    16501 tgggatgtaa tggcagaaga ttgggagaaa gatagtacag tagacattat tataaacaag
    16561 ttaatgagta gaacaaaaga aaattctata atttgtttac atgatgcagg tgaaaattca
    16621 ggtggtgcag taggtgcacc tgagcgtact attgaagctt taaagatagc tattccaaaa
    16681 cttaaagcca gtggtttaaa gttcgtaaca ccagaaagga tgtagttatg ataagtaaaa
    16741 ctgaaaagaa aaataaatgc ctaggtagtg tagctttttt agttttacta atgggcataa
    16801 caggatattt tgtttttaga gggcagtcag ttgaatcatt gataaaatca ttaaaaggtg
    16861 caagccctat gtttatacta attggatttg caatgatgtt tatatatgtt gcctgtgaag
    16921 gtataaatat atacttaggt atgaaagctt taaaccaaaa gactactctt ttaaaatgta
    16981 tgggatatgc ttttattgga ttttatttta gttctattac accttcagca tcaggtggtc
    17041 aaccagcaca agtttattat atgaaaaaag acgatataaa tatatcatat tcttcactta
    17101 tactattagt cattgttgtt attcaccaag tagttatact ggcatatagt ggaataatgt
    17161 tcattatgga acgtgagttt atattaaata atgtaagtgg tatgaacatt ttactaatat
    17221 atggtgtaat aacaaatgtt gctcttgtca ttggagtaat tgcaattata ttttcaaaaa
    17281 aacttgtaaa caattttata atatcaataa caaatttatt aggtaagtta agaataataa
    17341 aagatgttga aagttcaaga aaagttataa tatctcaaat tgaagagtat gttaaaggtg
    17401 ctcaatatat aaagcagaat ccaaaacttg tagtccaaat tttagtcata actattgtac
    17461 aaataacagc tatgttttta gttcctttct ttgtatacaa agcttttcat ctatcaacat
    17521 atactgtatt tgagatatta gcgatacaat ccttgcttaa tattgctgtt tcttcattac
    17581 cattgccagg ggctgttgga gcttctgaaa atagttttat gacattattt aagatatttt
    17641 tccctggtca cttattagtt ccggcaatgt tacttagtag gggaataagt ttttatgcat
    17701 ttgtagctat aagtggattg atatgtatag tagtacatgt aaaatcttct gaaaaagtaa
    17761 aagcagtgaa aaaagttgct tatgtaagat aatattatat agattatttt tattaatatg
    17821 tcaatttgtg tgtatatttt aattaagctt taaacatatt tacaggagta gaaactatta
    17881 aaacgtgtga cattgctaag cttttgctaa taaagtataa tattttatat agtttgaaat
    17941 atttaagaaa tatagcaaaa aaaggagctt ggtgatgaat aaagagattc caaaaaaaat
    18001 tttagtagct gatgataatc ctgagattag ggaaatcgta gaaatacttt taactggtga
    18061 aggttatgaa gttattatgg caacgaatgg agaagaagct gtaaacttag tagatagtac
    18121 gattgatttg attattttgg atgttgtaat gcctgtaaaa acaggatttg ttgcatgtaa
    18181 tgaaattaga gaaaaaacga cagcaccaat attattttta actgcaaaaa ctcaagactc
    18241 tgataaagtt attggatttt cagcaggagg agatgactat ttatcaaagc ctttttcata
    18301 ttctgaacta atttcaagag ttaagtctct acttagacgt tattatatat accagggaaa
    18361 ggaaaaggaa agagagccaa atatattaaa aacaaaagag ttatgtgtca atttagatac
    18421 tcaagatgtt acactaaatg gagaacctgt gtttttgaca actatagaat atagtatatt
    18481 aattcttatg ttgaaaaata gaaaaaaagt tttttcgtct gaaaatttat atgaaactat
    18541 ttgggatgag cagtattttt atacagcaaa taatacaata atggttcata ttagaaattt
    18601 aagaaaaaag ttagaaaaag accaaaaaaa tccacaatat ataaaaaccg catggggaaa
    18661 ggggtattat attgattaac aaaattaagc catctaagtt gggagttata ctattgatat
    18721 atgtagtaat ctcagctgca gtggctttca ttttaaatat accattttac tctgctgttg
    18781 aatctatagt aaaagatgta tcagaaggga aagtatattt taaaagagaa gtaaatcaac
    18841 ttatggataa ttttcaaact tatgtgaata acaatggtat aagagcagat gatggagatg
    18901 atatagcaaa atggaatagt gataattggt ttgtttctat gaagatatat aaaggaaata
    18961 aggtttttta tgatacatta tactatccta atgaagtacc tgataagagt gaggaaaatg
    19021 agttttatag cagtcccatg gctagaaact atgagataca attttctgat attaaagcta
    19081 atatagttgt agtagcttta tttagattta gatttcaaaa ttttatagaa gcaatttcta
    19141 ttacatttat ggcacttaca tttattataa catttttact gttgtttagt agaaagataa
    19201 agtacctaag gaaaatagaa atgggtataa agatagtaga gagtggcagt ttaaagtata
    19261 gaataccagt aaaaggtaat gatgaattat catcacttgc atctagtatt aatgagatga
    19321 gtcatacatt ggagaatcaa cttataaaag aaagggagat gaagaataaa aataaagata
    19381 tagtaacttc aatatcacat gatgttagaa ctccattaac gtcagtaatt tgttatttag
    19441 atttgattaa agaccataaa tacaatagtc ctgagcaatt ggaacaatac ataaaaaatg
    19501 taaggtctaa agcatatcaa ataaaagatt taacagatga cctttttacg cattttgtta
    19561 attctggaga ggaagtttca aaagggtatg aaaaaataaa tgctaatgaa tttatttttc
    19621 aattactttt agatagttca tatacattgg aggagaaagg atttcatata acaatagaaa
    19681 atgaacttta taaccaatat catatttatg tagatgttac acaatttaga aggatatttg
    19741 ataatttatg ttctaatata ataaaatatg ctaggaaaga taagcctata gtatttttaa
    19801 ttaaagtaga ggattctaaa ttactaatta tacaaaaaaa tagagtaagt aatttttcta
    19861 agaatgtaga aagttttgga attggtataa agaattgtga gaaaatagtt gcatatcata
    19921 atggaagcat aaatgtaaat tcagatgata aagaatttgc tattgaaata tctattccag
    19981 caaaagaatc ataaaaattt aaattatata ttgtgtaatt gctattatat attgtgtaat
    20041 tgcttatgta aaattctatg aagcaggaag catgtgactt tagttatggg atgttcaata
    20101 ttgtgtacct ctaggcaagt aacaagttta cttacaagac catataatat tattatctta
    20161 aaaaaagggg acataataat atggttttga caggagcaca ttatatttac attctattta
    20221 tggtaattat attaattact atgattatga aaaaggatac aatagtacca tgtatattag
    20281 gtgttttttt tatggggttg ttctttgaaa aaaacatttt tggtgctata acagcagtgt
    20341 ttaactcttt tattatatct ttaaatgagt tgggtccaat tatcttgatt atagctatta
    20401 tggttgcatt atctaaagcc ctagaagcca ataatgctat acagtatatg gtgagaccat
    20461 tttcgagagt tataaaaaat tctaatacag cattttttgt aactggattt gttatgttag
    20521 ttctttcttg gtttttctgg ccaactccag ctgttgcctt agttggagct gtatttttac
    20581 ctgttgctat gagagctggg ctaccagcaa ttggtgttgc agtagcatta aatttatttg
    20641 gtcatggtct tgccttgtct acagactttg ttattcaagg agctccaagt ataacagctg
    20701 gtgcagcagg tgttgcagta tcatatgtta taaatgatgg tatgattttg ttttgggtga
    20761 tgggaatcgt aaccatatct atggcatttt atacattaaa aagagatatt aacaaaggaa
    20821 tgtttagaga agaactaaaa ggttttgaaa gtgaagaagt agaagagttc aatggaaaat
    20881 ctaaaatagc aactatttta gtagctctgg gatttcttgc tgatataatt gctatgtata
    20941 tatttgacct aaaaggagga gatgcatcag ctcttttggg tggaacagca gtatttttaa
    21001 taataattat aaatgttata aattttggca aagatagtct tgaaaatgta tgtgagaata
    21061 taattgatgg ttttgtgttt gggataaaaa tatttggagc aattatacca atagcagcat
    21121 ttttctatat gggtgaagtt gcaccattga caggtgtatt tggaaaagta ttagcaccag
    21181 gttcacaagg tttattatca gatataggca tagctctttc tcaaacaata ccacttaata
    21241 aatttgccgt atcaggtata gaaactgtag tgggagcaat aactgggctt gatggttctg
    21301 gattttctgg gatttcatta gttggttcac ttgcctctgt atttggaact gctataaatg
    21361 caagtgttgg tgcactagct gcattaggac aaatcagtgg tatatgggta ggtggaggct
    21421 gtctagttcc ttggggtctt atttcagcag cggcaatttg tggagttagt ccaatagaat
    21481 tggcaaagcg aaactttgtc ccagttataa ccggattggt tgtaacaacc atagtagcaa
    21541 tgtttattat ttaataaatc gaataaatct ttaattgtat aaattaaaaa tagatgtgta
    21601 gctataattt attataattg ttttactaat tctaagccat acatctattt ttattgatat
    21661 ttttattagt attctttaat aaaataatat ttttcattat actatacata acatctaaat
    21721 ataacatcta aaatactgtt atctatattt aatatcttgc caaatttttt gatgttttat
    21781 tgccctgtcc caccaacata tataagctat cacaagtttt tacctatact atcaccaaac
    21841 aaaaattcta attttaattt attattttat aacctatatt atattaacta tattagtttg
    21901 tagactttag atgtaattat aaatattaat actccaagag ttcaaattca tatcctagtt
    21961 ttcttgcatt tgatataaaa tcatttaaaa tttctgtact agttttggaa actgcatgta
    22021 gtaacaagat agaaccatca tgtagattat ccattatttt ttgcgttgct tcttcatgtg
    22081 atggttgttt atcagtatcc cagtctctgt atgcaaaact ccaaaataca gtcttgtatc
    22141 ctaaattttt agtcattgcc aagctttttt ctgagtattt tcccatagga ggtctaaaaa
    22201 atttaaccat atctttccca gttacatctt tatataactt ttcaacatca tataattcgt
    22261 cattaaagtt ttttagattg cttgtttttg ttggcataga tggatgggtt ttactatgat
    22321 ttccaactat atgtccctca gaaaccattc tttttactaa atctttattt tcttttatat
    22381 atggagctgt aacaaaaaaa actgctttaa cctgattttg ttttagagtg tcgagtattt
    22441 ttgtagtgta tccattttca taaccttcat caaatgtaag atatagtgtt ttttgaccat
    22501 cttttgtagg tccattgtat agggcatcat agtcagaaaa tttaaattca atgtccgtat
    22561 ttacttctgg tgttttatgc ttattgtttg gtatataaaa ccaatcaaga gtagtgttat
    22621 ctaaagaaga tacatctaat ttattattat cattattagt agtggctttt gatggtttat
    22681 ctgttccgtc tgaaactatt acattggaag tatctttacc actatctatt ttttccttat
    22741 cttcttgtaa ctgtgtttcc ttattttgat tttcgttttg attgttttgt gaatttgaac
    22801 atccaacaat aaaaaaaata gaaatagaaa gtagtgttaa ataatgcaat acttttctaa
    22861 acattttgaa gctcctttct aaattaataa taattgcata taataataat tatatgatta
    22921 attacaattg catatagtat tattataatt ggaaattact attaataata gttatatctt
    22981 taaaaataag gtcacaatag tatgacaaag ggtgaataat caataaattt aaaaaaaaat
    23041 gaaaaaaata gttgaccata ttatgagaag atgttattat aataaatgtg ctcaacaaga
    23101 agtaacaaaa taacaagcac aaagaagtta attgaat
//
LOCUS       DAYASV010000054        22825 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYASV010000054 DAYASV010000000
VERSION     DAYASV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.054"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            16..387
                     /locus_tag="ACGI68_002024"
     CDS             16..387
                     /locus_tag="ACGI68_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0138462.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            519..1793
                     /locus_tag="ACGI68_002025"
     CDS             519..1793
                     /locus_tag="ACGI68_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0138463.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            1904..2848
                     /locus_tag="ACGI68_002026"
     CDS             1904..2848
                     /locus_tag="ACGI68_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138464.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3064..4401
                     /gene="rsxC"
                     /locus_tag="ACGI68_002027"
     CDS             3064..4401
                     /gene="rsxC"
                     /locus_tag="ACGI68_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFH0138465.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4424..5401
                     /locus_tag="ACGI68_002028"
     CDS             4424..5401
                     /locus_tag="ACGI68_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HFH0138466.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5401..5970
                     /locus_tag="ACGI68_002029"
     CDS             5401..5970
                     /locus_tag="ACGI68_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HFH0138467.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            5983..6576
                     /locus_tag="ACGI68_002030"
     CDS             5983..6576
                     /locus_tag="ACGI68_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit E"
                     /protein_id="HFH0138468.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6591..7166
                     /gene="rsxA"
                     /locus_tag="ACGI68_002031"
     CDS             6591..7166
                     /gene="rsxA"
                     /locus_tag="ACGI68_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFH0138469.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7182..8153
                     /locus_tag="ACGI68_002032"
     CDS             7182..8153
                     /locus_tag="ACGI68_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFH0138470.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8373..8957
                     /locus_tag="ACGI68_002033"
     CDS             8373..8957
                     /locus_tag="ACGI68_002033"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFH0138471.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            9002..9661
                     /gene="radC"
                     /locus_tag="ACGI68_002034"
     CDS             9002..9661
                     /gene="radC"
                     /locus_tag="ACGI68_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFH0138472.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9731..10792
                     /locus_tag="ACGI68_002035"
     CDS             9731..10792
                     /locus_tag="ACGI68_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HFH0138473.1"
                     /translation="MAKEKKKEKKGFFSFNKMTKDMGIDLGTANTLVYIKGQGIVVRE
                     PSVVAIRDDSKEVLAVGEEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQK
                     AADKKGVVSPRIAICVPFGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVE
                     EPEGNMVVDIGGGTSEIAVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERT
                     AENVKINIGSTFKDDEEINMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIK
                     STLERTPPELASDIMENGIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTG
                     KSVEDQEIFEKVLMMNTKN"
     gene            10802..11695
                     /gene="mreC"
                     /locus_tag="ACGI68_002036"
     CDS             10802..11695
                     /gene="mreC"
                     /locus_tag="ACGI68_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFH0138474.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11710..12198
                     /gene="mreD"
                     /locus_tag="ACGI68_002037"
     CDS             11710..12198
                     /gene="mreD"
                     /locus_tag="ACGI68_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFH0138475.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12210..15188
                     /locus_tag="ACGI68_002038"
     CDS             12210..15188
                     /locus_tag="ACGI68_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFH0138476.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15286..15969
                     /gene="minC"
                     /locus_tag="ACGI68_002039"
     CDS             15286..15969
                     /gene="minC"
                     /locus_tag="ACGI68_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFH0138477.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            15993..16790
                     /gene="minD"
                     /locus_tag="ACGI68_002040"
     CDS             15993..16790
                     /gene="minD"
                     /locus_tag="ACGI68_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFH0138478.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16808..17092
                     /gene="minE"
                     /locus_tag="ACGI68_002041"
     CDS             16808..17092
                     /gene="minE"
                     /locus_tag="ACGI68_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFH0138479.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17177..18307
                     /gene="rodA"
                     /locus_tag="ACGI68_002042"
     CDS             17177..18307
                     /gene="rodA"
                     /locus_tag="ACGI68_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HFH0138480.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18348..18761
                     /locus_tag="ACGI68_002043"
     CDS             18348..18761
                     /locus_tag="ACGI68_002043"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFH0138481.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            19246..19575
                     /locus_tag="ACGI68_002044"
     CDS             19246..19575
                     /locus_tag="ACGI68_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0138482.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            19579..20370
                     /locus_tag="ACGI68_002045"
     CDS             19579..20370
                     /locus_tag="ACGI68_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFH0138483.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFVFGLLCIIAYFILRFKISLIKNNN"
     gene            20500..21320
                     /locus_tag="ACGI68_002046"
                     /pseudo
     CDS             20500..21320
                     /locus_tag="ACGI68_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428476.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(21532..22725)
                     /locus_tag="ACGI68_002047"
     CDS             complement(21532..22725)
                     /locus_tag="ACGI68_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HFH0138484.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
BASE COUNT         8723 a         2673 c         4424 g         7005 t
ORIGIN      
        1 gagagtgatt tcaaaatgaa gtttaatttt tgtcataaca atttcaatgt tactaattta
       61 gaaaaaagtt tagattttta taaaaaagct ttagggctta aagaggtaaa gcgcaaggaa
      121 gcagaagatg gaagttttat attagtttat ttaggagatg gcattactag tcatacatta
      181 gaattaactt ggcttagaga ttgggataga ccatataatt taggagacaa tgaatttcat
      241 ctggctttag aagttgagga gtttgatgag gcaaaaaaac ttcataaaga tttagattgc
      301 atatgttttg aaaatgaaag tatgggaatt tattttatag cggacccaga taattattgg
      361 attgaaatac ttcctaaaaa tcactaattt taaggatatg tttataattt ctttacaatt
      421 aataagaaaa taagttatca ttaaattgta gggactaaaa atggataata atgttatgaa
      481 tttatgacta taaaaatttg ttggggggag tgatagtagt gaaaaaagct atagcagctt
      541 taggaattgg ggcagtagca gtatctgtta gctccataaa tgcaagtgct cttgaaaagg
      601 gaacagtaac agcaagtgcc ttgaatataa gaagtggacc aagttctgat tgcgataagg
      661 tagcaaaatt atacaaggga aaaactgtag agattttaga aaagtctaat ggatggtata
      721 aagtaagggt atcgagttct gttgttggat ggggaagtgc aaaatacata tcaacaagtg
      781 gttcgtctga aggtacatca agtcaaaata atccaacttc aagtgggaca actattagtg
      841 gaaatggtaa agtaaatgtg agttctagac tgaatgtaag aagtggtgca ggtactaact
      901 attcattagt tggtaaagca aataatggag atgtagtaaa gttactagaa caaagcaatg
      961 gatggtataa aataaaacta tcaaatggag ttactggatg ggctagtagc caatatattt
     1021 ctaaaacatc agaagatgtt ggaacgaaca attctagtaa ttcaaatagt actaataatt
     1081 ctgataaaaa accatctagc gaagaatcaa ttgaaggcaa gaatggtaag gtaacatctg
     1141 ctgtgagttt aaatgtaaga agtggtcctg ggacaagcta ttctataata ggaaaattaa
     1201 atggtggaga tgttgttgaa ttaaaagcta aaagtaatgg ttggtataag gtaaagttat
     1261 cgagtggaac aattggatgg gtgagtgcca gttatatttc tgagactaat gaggatacaa
     1321 aagaaaaacc aaattcatca tctaaccaaa attctcaatc taatagtaac tcaaagccat
     1381 catttactgg aaattcagat aaatctacag caaaaggttc aacaatcgta gattttgcat
     1441 atactcttat tggaatacca tatcaatggg gagcttcagg acctgataaa tttgactgtt
     1501 ctggatttac acagtatgtt tttaaacatt ctgtaggcgt ttctattcct agagtttcta
     1561 gagaacaggc taattttgga agtgcaattt ctatgggaaa ttatgcacca ggagatttag
     1621 tttattttga cactgatggt gatggaacaa ctaatcatgt aggtatatac gtaggaaata
     1681 gtaaatttat ccattgtagt ggtactcaga ctaatcctaa taaggttaaa gtggataatt
     1741 taactagttc atattggtct aaagtattac ttggtgcaag aagatttgtt tagtaagtac
     1801 aatttcttat taaaaaatta gaagaaataa ttatttatat tcataaattg tgactcttaa
     1861 aaatatactt tattaataat ataattttgg gagggacaaa ctaatgaata gaagaaaaac
     1921 gatatacaga ggatttaata ataatagaca taagattgat ttacgtaaat atgttataac
     1981 gattgcttgc ttgtgtctaa ttggctatta ttcttataca aaaataaaag atagcaagat
     2041 attggagtat gtatcggcta aaataccatt tctaaataat tcatcagata taacttataa
     2101 agatatatct gatgaattaa attcaataaa aaaagggaaa aaatctaaat cacaaaccaa
     2161 ttcagatgat aaacaagaaa ctaatccaga aaaagcggta aataacacta aagagccaga
     2221 agaagtcaaa ttagctacta tagaaggctg ggatatgtat actattcaag ttgctgcaat
     2281 agataataat gatgatttga aaaaaataca gacatcatta gttaataatg acataccttt
     2341 ttcagttatg gagaaggatg gagtaaaaaa aatacagacc tattcctctt ttgatgaaaa
     2401 tgatgttaga aagcagataa gcagtgtaag aaaggtgttt ccagatgcat ttttatcaca
     2461 cttagatgca cctatgttgt ctttagagta tacaagtaac tacgcttata tagagagtat
     2521 atctaaagaa ttaaataaat taataacaaa ctttaaagaa gaatcaagtt tttggtcgaa
     2581 tgctgaaaat aatgtagata tggaaaaata taatactatt ttgacaaata gaaaggcaat
     2641 atcacaaaat atatcaaaag aagctgaaaa gattgattat tctgaaatgc gtttatttaa
     2701 agataattta atagagtatg taaagaatgt gaatgaaaaa attgatacag cttcaaaagc
     2761 agcaaatgaa gaaaaatata gtgtaagtaa aagtctgctt ttatcatcca tgcaggagta
     2821 ctctatgttt ataaattcta taaaataaaa tacagaaaat ttggacttta gaaagctata
     2881 tgaaaaggat tgtttaatta agcaatcctt ttcctcaatt tgacttaata tagaggtgtt
     2941 gatttataaa ataaattata aaaatacgga tattttttta acaattgtta agatttttta
     3001 atatatatag tatacttata gaaggcagaa aaaatgtttt tttatagata agggggtaaa
     3061 acaatgaaac tcttaacttt taagggaggg atacatcctc catatagaaa agagtactct
     3121 aacacaaagg cacttgaaaa agctcaagct ccaaagatag tttacattcc tcttcagcaa
     3181 catatagggg caccagctaa acctattgtt gaagttggtg atgaagtaaa gtttgggcaa
     3241 aaaataggtg aacaacaagg ttttgtttca tgtaacgttc attcttcagt atcaggtaaa
     3301 gttattgcta ttgaacaaca tgaagtacca ggaggttcag ctcaatgtgt agttatcgaa
     3361 aatgacttta aggaagaatt acatgagagt gttcaaccaa aaggtcagtt agaggatttg
     3421 agcaaagaag atatagtagg tatcataaaa gaggcaggaa tagtaggtat gggtggagct
     3481 acattcccta accatgtaaa agtatctcct ccaccagata gcaaggcaga agtagttata
     3541 ttaaatggtg cagaatgtga accatactta actgcagacc acagattaat ggttgaaaat
     3601 ccagaagatg ttgtgtttgg tttaagagca ttaatgaagg tattagatgt caaaaaagga
     3661 tttataggaa ttgaaacgaa taaaccagat gctatagaag caattcaaaa tgtagctaaa
     3721 gactatagcg aaatcgaagt tgttggcctt caagtgaaat atccacaagg agcagagaaa
     3781 caacttattt atgcgtgtac aggcaaagaa gttccatctg gtggattacc aatagcagca
     3841 ggtgcagttg ttgacaacgt tgccacagca gcacagatag ctaagtctat taaaacaggt
     3901 atgcctttgg ttgaaagaat cactacaata acaggtagtt gcatcaaaga gccaaagaac
     3961 ctaataacaa aagttggaac attagtttca gaaataatag aacaatgtgg tggatttaaa
     4021 gaagataaga agattggtaa ggttataatg ggtggaccta tgatgggaat agctcaatat
     4081 actactgaaa tagcgactaa caaaggttct tcaggaattt tatgtttgga tgaggaagaa
     4141 tcacgtactc cagatattca aaactgttta agatgtggaa gatgtacaga tgtatgtcca
     4201 tcattcttac aaccactttt cataagtgca tattctttaa aagatgacta tgacacagct
     4261 gaatatcata gagcaatgga ttgtatagaa tgtgggtctt gctcatttat ttgtccagca
     4321 agaagaccat tacttcaatc tataagatca gcaaagagag aaataggagc aaagagaaga
     4381 aagcaagctg ctcagaaata aagaaagtta agggggaaaa acgatggaaa ataagttgat
     4441 agtatcatct tctcctcatg tgagaagtaa tgaagatact tcatatataa tgaaacaagt
     4501 tattatagca ctccttccag cagcagtagc aggagtatac ttctttagac ttaatgcttt
     4561 gagtgctatg tttttttgta tacttggtac agtaggtact gaatttttat atcaaaaatt
     4621 tacgaagcaa aaaagtacta taggagattt ttcagctgtt gtaacaggat tattattagc
     4681 atttaacgta ccagcatcac ttccttggtg gatgtgtcta gtaggaggaa tatttgcaat
     4741 attagtagtt aaaatggtat ttggtggaat tggatgtaac tttgtcaatc cagcacttgc
     4801 tgcaagagca tttttattag catcattccc agtagcaatg actgcttgga ctcaaccagg
     4861 tgttaactgg ataggtaaaa atttagatgc agttactaca gcaactccat taagcttttt
     4921 gaaaaatgga gcagcaggac tggcagacct ttctagcaat ggaattagtc ttgctgacat
     4981 gatgataggg aatattggtg gatgtatagg tgagacatca gcaatattat tattattagg
     5041 tggagtatac ctaatgtata aaggcataat aaattatgtt ataccagtat tttacatagc
     5101 aactgtattt atattaacat tccttttagg tggatttaat ataactttcg caatatatca
     5161 actgtttgct ggaggactta tgttaggtgg attcttcatg cttacggatt atacaacttc
     5221 gcctatgact aaaaaaggtc aaataatata cgctgtatta gctggtctta taacaactgt
     5281 tataagaatg tatggtggat atccagaagg tgtatcttac tcaatactat tagtgaactg
     5341 tcttgctcca cttatagata agtttgttag aaatagagta tttggggagg tggctaaata
     5401 atgaacagta tggtgagatt aggtggtact ttacttgcta taagtgcaat agctgcatta
     5461 gctcttggag ctactaatca ggttacagct ccagtaatag aacaaagaaa tatacaagca
     5521 aataatgaac ttagaaaagc agtccttcca gaagcaaaag aatttaaaga aatgaaggaa
     5581 gatacttaca aaggccttgg agatggtctt atagctgaag tgtatgaagg tttagatggt
     5641 tctgaagtgg ttggatatac tttaaaagct aaaccaagtg gatatggtgg agaaattgaa
     5701 gttatggtag gtatatcatc tgaaggtcaa gttacaggtg ttgacattgg taatatgtca
     5761 gaaacagcag gtcttggagc aaaagctaag gatgatgcat ttaagggtca atacaaagga
     5821 aaaactgctg agcctcttga agtagcaaaa ggaagtacaa cagcagataa tcaaatatta
     5881 gcaatatcag gtgcaactat tacatctaca gcagttacta caggagtaaa tgctgctata
     5941 gatgttttca atagtgctct taataaataa ggagggttaa ttatgaattt agctaaagta
     6001 tttaaaaatg ggctaataga tgagaaccca acatttgttc aagttatagg tatgtgtcca
     6061 acactagccg ttacaacttc agcaataaat ggaattggta tgggtctttc aacagcagca
     6121 gttttaatat gtgcaaattt agttatatct ttaataagaa aaattacacc agataagata
     6181 agaataccta tatttatagt agttatagct acatttgtta caatagtagg tatgctttta
     6241 aaagcatatg ttcctgctct tgataaggct cttggtatat atataccatt aatagttgtt
     6301 aactgtttaa tattagctcg tgcagaaagt tttgctttca aaacaggagc tatgccttca
     6361 atagttgatg gtgtaggtca aggtcttgga tttactgttg cacttacaat aataggtgca
     6421 gtaagagaat tacttggaaa tggtagttta tttggaatga cattatttgg agcatctttc
     6481 caaccagtat taatatttat attaccacca ggtgcattct taacattagg attcttattt
     6541 gcaggattta ataaattaag aagtaaaaaa gcatagtgga ggtgtaataa atgaatttaa
     6601 tacttttatt tttaagtatc gttcttgtta ataacgttat aacatctcaa ttcttgggta
     6661 tatgtccttt cttgggagtt tctaagaagg ttgatacagc agtagggatg ggagttgcag
     6721 ttacgttcgt tttaactcta gcttcaatta taacttattt tatacagatt ttattagtaa
     6781 aaactggaac tggattctta caaacaatag cttttatatt ggttatagct tctatagttc
     6841 agttcgttga aatggtaatt caaaagatga gtccatcttt atatcaagca ttaggagttt
     6901 atttacctct tataactaca aactgtgcag ttcttggtat agctctagtt aatgttcaaa
     6961 aaagttataa tttagtagaa acaataataa acggatttgg agccggagct ggatttactt
     7021 tagcaatagt aatattcgca ggtataagag aaagactaga attagctgat ataccagaag
     7081 catttaaagg tttccctata acgcttatat cagcaggttt aatgtcaata gctttcttag
     7141 gatttacagg acttataaag ctataaaaaa gaggggtgaa aatggtgata cttacagctg
     7201 tattagtttt aggaattatg ggcttaatct tcgggatagt actagatttt gcatctaaaa
     7261 agtttgctgt agaagtagac gagagagtgg aagctatact aggagtactt ccaggagcca
     7321 actgtggagg ttgtggattt ccaggttgtg gaggtcttgc aaatgcgata gttgaaggca
     7381 atgcaccagt taatggttgc cctgtaggtg gagcagatgt tggtgcaaaa gttggagaaa
     7441 taatgggaat aagtgctgaa gcaggtgaga aacaagtagc aaaagttatt tgtaaaggaa
     7501 cttgttcaag tgcaaaagat aaatatgagt atgaaggaat atcagattgt agagcagcaa
     7561 atgttttaaa ctcaggtgct aagatgtgta aatttggatg tttaggtctt ggaacatgta
     7621 aagatgcttg taaatttgat gctatttcta tagttgatgg aatagcagta atagatgaag
     7681 aaaagtgtgt aaattgtgga aaatgtaaag aagtttgtcc taagggtata ataatcacaa
     7741 aaccagaaag tcaagaagta gttgtagagt gtaatagcaa agagtttggt aaagctgtta
     7801 aagaaaaatg tactgctgga tgtattggct gtggaatgtg tgtaaaagct tgtaaatttg
     7861 atgcaataat atttgaagac aaaatagcta agatagaccc taataagtgt gtaggttgta
     7921 tgcaatgtgt agcaaaatgc cctacaaaag taatatcagg tgacataact aagaaaaaga
     7981 aagttactat tgaccaagaa ctttgtgttg gatgtacagt gtgcaaaaaa caatgtaagt
     8041 ttgatgcaat agagggagaa ttaaaagaaa aacataaggt agatgcagat aaatgtgtag
     8101 gatgccattt atgtatggaa aaatgtccaa agaaagctat taaaattttg taatatggac
     8161 aaaataagga tatggaataa tttccatatc tctaatgagt acataaaaca gaggtatgga
     8221 ataatttcca tacctctatt ttatgtatat ttattctaat ggtaataaat ataacaatag
     8281 tgtaacaaaa aaggttcgac atttctgttg aattttttac aatatagtga tagactaaat
     8341 aggtacaaat tacgtacaaa tgaggtgaat aaatgaatat aatcttggca tcagcctctc
     8401 caagaaggaa agaaattcta gagaatacga atgtaagatt tgatataatt aaaaatgaaa
     8461 ttgatgagat tatattggaa ggtgaaacac caaaacattt agttatgaga ttagcttttg
     8521 aaaaaagtat gtctgtagca tctgaacata atgaagatgt tgttattgga gctgatacag
     8581 ttgttgtttt agacaatgct attttaggaa aaccaaaaga tgagtcttgt gcaagagata
     8641 tgttaaaaag actatctgga agagagcatc aagtcataac aggtattagc ttaataaact
     8701 tgtgcgaaga taaaaaggta attgactatg taatcagcaa tgtgaaattt aaaacgttat
     8761 cagagcagga tattgaggat tatctgaaaa ctaatgagtc atttgacaaa gcaggagcct
     8821 atggtataca aggttatgga gctttgttag ttgaagaaat tagaggagat tattttaata
     8881 tagttggtct tccaatttca aggttaggcg acctattaaa aaaatatttt agtataaatc
     8941 ttttttatgg agtgtgattt atctgaaaaa gtcatttaat tcaaccatta aggtaaaaga
     9001 aatggcacca gaggaaagac ccagagaaaa gatgcttgcc aaaggcgtaa aaagcttatc
     9061 aaatgctgag ttgttggcta tacttttaag aactggcaac aagaataaaa atgctattga
     9121 attagccaac tatataataa atagagatat tcagggtatt agacatttag aagatatgac
     9181 aatagaggag ctgtgtaata tagatggaat tgggttatca aaatcaaccc aaattaaagc
     9241 agctttagaa ctaggctcta gagtagctag ttttaaacct ataaagtata aaataaggaa
     9301 tccttgggat atacagaggt attatatgga tagcctaagg tatttaaaaa aagaggtttt
     9361 taaagcggtt cttttaaaca caaaaaatga gataatatct gatgtagatg tatctatagg
     9421 gactttaagc tcgtcattag tccatccaag agaggttttt aaagaagcta taagaagaag
     9481 tgcaagtaag ataatagtga tgcataatca cccttcagga agtgtagaac catcaagaga
     9541 agataaaaat atcacatcaa gacttattaa atgtggagaa ataattggaa tagaaataat
     9601 agaccatata attattggag atggattata ctttagcttt aaagaaaata tgataatttg
     9661 actttatatg tgataaaata atagaagaat gttttaaaca tgattcttaa tagtaggaag
     9721 gagtaaaatc atggctaagg aaaaaaagaa agaaaaaaaa ggttttttta gttttaataa
     9781 gatgacaaag gatatgggaa tagatttagg gactgcaaat acattagttt acataaaagg
     9841 acaaggtatt gttgttagag aaccatcagt tgtcgcaata agagatgata gtaaagaggt
     9901 attagcagta ggtgaagaag ccaaaaaaat gataggtaga acaccaggaa atatagtagc
     9961 tataagacct atgaaagatg gagtaatagc tgattttgac ataactcaat caatgattag
    10021 ctattttata caaaaagcgg ctgataaaaa aggtgttgta agtccaagaa tagctatatg
    10081 tgttcctttt ggagttacag aagtagaaaa gagagctata gaagaggctg caagacaagc
    10141 aggagctaaa gatgcattcc ttatagaaga gccaatggca gcggctatag gggctggatt
    10201 aaaagttgaa gaaccagaag gtaatatggt agttgatatt ggtggaggta cttcagaaat
    10261 agctgtaata tctttaggag gaatcgttac tgctaaatct ataagaatag gtggagacga
    10321 atttgacgag tctatagtgg cttatgtaaa aaaagaatat aatcttatga taggtgaaag
    10381 aactgctgaa aatgtaaaaa ttaacatagg ttctacattt aaagatgatg aagaaataaa
    10441 tatgcaaata agaggtagag atttaatctc aggattacct aaaacaatag aaatatgctc
    10501 tacagaagtt agagaagcat taaaagaacc tgtaagttca atagtagatg caataaaatc
    10561 tacactagaa agaactccac cagaattagc ctcagatata atggaaaacg gaataatgct
    10621 aacaggagga ggcgctttgc ttagaggtct tgataagctt attacacagg aaactggtat
    10681 gtctgtacaa atagcagaaa cacctcttga ttgtgttgca cttggaacag gtaaatctgt
    10741 tgaagaccaa gaaatctttg aaaaagtatt aatgatgaat actaagaatt agaagttggt
    10801 gatggccttg agatttgaca agaacaaaaa tgagaaaaaa aggattaatg ttaaagtgat
    10861 agcaacgggc gtagttgcta tcactttaat tggaattgtg ggtatatcaa ttggtaaatt
    10921 ttctagtggt agtccagtaa atttaggagt agcttcagat gctattacaa gtgtaggtaa
    10981 gggaataaat gatggatttt cttttatcaa aaatggtttt aaagatgtag caaattttaa
    11041 agataattct aaaaaggtaa aaaaattaga agaagaaaat gaaaaactta aaaaaaatat
    11101 gatagcttta aatgccaaac tagataaaac agaatcgcta gaagagttaa aaaagacact
    11161 gaactttgta caagaagagt ataaggcgac aagtatttca gcaagtgttg ttggtaaaaa
    11221 tgatggaaac tggtatgaga gttttgtcat aggtgcagga aaaaattcag gtgtaaaaaa
    11281 agaaagtata gttatgaatg gtagtggatt agttggcata gtttatgaag tttccaacaa
    11341 ttatagtaag gcaatttcac tattggattc taaggcatct gtaagcttta aactagcaaa
    11401 ggatgccaat gctaaaggta caattactca aaatactact ttagataata aagatagtta
    11461 taatagtaaa ggatatttgc aaggatatat gtttgactca tcatataatg taattcaagg
    11521 agatataata acaacatcag gtcttggatt cttcccagat ggtataccaa ttggcgaagt
    11581 tgaaaaagtg gttgatgata aagataagtc attaaaatat gtagttgtaa aaccttatgt
    11641 tgattttaag aatataaatg acgttgtagt catagaacca agaaatatag gataaagggg
    11701 ataaaaacaa tgaaaaaagt tttactttgt ctgttgggga ttgtgcttat gactatagaa
    11761 aatagtataa caaattatat agatattttt ggcataagct ttaatttagt tcttatatat
    11821 gtaaccataa tctctcttta tttagatgaa ttagaaggtg ggattatagc agcaataata
    11881 gggcttatta aagacataac cgttggtgga atttttggtg taaatgcatt aattttgttt
    11941 attattgcat atgcaattgg atacatgaga gatcagctat ataaaggtag ttatattatt
    12001 actttcgttt taatttttat aggcagttta attgattcaa ttgtcaatat aggtacatct
    12061 agtataattt atcaaagcta caacatatca acactttttg taaaaggtct actagtagca
    12121 ccaatagtga atagtttaat tggtttagta atatatcgct tatctaaaag agcagtttta
    12181 aaacttaaag aagattagtt ggtgatttca tggaagaaaa taaacaagtt gatagactaa
    12241 aaataattaa gatagtcatg ggcgtgatat ttttagctat tttagtaaaa ataatctata
    12301 tgactacatt taaatatgaa tattacaatg agttagctga gaataaaaca tataaaaagt
    12361 tggcaataga agctccaaga ggagaaataa aagatagata tggaagactg ttggcaggaa
    12421 ataaaaactt atttactgtt caagtttcag gtaacgatat taataagaaa gatgcaaata
    12481 agcattcaag agcaaatgaa atatcattaa aacttataaa tttgctggag agaaatggtg
    12541 aagaatatgt agatgagttt cctatatatg tagaaaatgg aaagtactat tatacatatg
    12601 acagagatat tagggaatat aaatcagaaa atggtattcc aaatgattat aatgctaagg
    12661 aaagttttta ttacttggtg gacaaactta tatcagctgg aatattatct caagaagata
    12721 aaagattgga tgcgacaaga ttacaggcaa aactaaatga aaatggatat tatccaccta
    12781 ttttagttag taaatggatg ttcacagcag aaagagataa gagagattgg ttggctagtt
    12841 ataagataaa agaaactaaa ctgagtgcca aagaagcgtt tgaaaaagta agaaatagtg
    12901 atgcattaga aattgataaa agtttgagtg atgaagatgc aagaaaaata atggtcgtaa
    12961 gggatttgat aaaatcaaaa ggatattctc aatataatcc agtaactatt gctaaggatg
    13021 taggagaaac tacgattgct caaattgaag aaagtgctat ggaccttgtt ggtgtttcaa
    13081 tagcagtaga accagtaaga tattatccta atgggtcatt agcttctcat atgttaggat
    13141 atgtaggaaa aatgccatcc actcaaatag aatcatacct tcaaaaaggt tatgaaactg
    13201 gagatatggt aggtcttgca ggagtagaaa agagtaatga aagtagatta agaggaactg
    13261 atggttataa aatggttaaa gttgatgcat tgggtagaat cagtaaagaa attgaatcta
    13321 aaaaacctaa atcaggagat actgtgtatc ttacactaga taaagattta caagaagttt
    13381 cagacaatgc attaaaacag ataatcgaag ttgcttctaa aggaggtact tttaaaagta
    13441 aatttgggga caaaccaatt agtgcatatg caggaaaagc acagtcagca gctcttatag
    13501 ctattgatgt taaaaatgga gaagtattag cttcatcaag ttatcctaat tatgacccaa
    13561 ataaatttgc aaaaggtatt tctacagagg attataaagc acttcaacca aaaaatccaa
    13621 atgacttact agcaggaagt ccacttttaa acttggttac acaaggggaa tttcagccag
    13681 gttcttcatt taagatggta acttcaatgg cagcgttaga aaatggtctg gatccaaact
    13741 ttactataaa tgaccctggg gttataatgt taggtaaaaa atcttttggt gactatgtat
    13801 ggaaccatgg tagaggaaac catggtatga caaacttata taaagcaata caagagtctt
    13861 gtaatatata tatggcaaca attggtacag gtaaaacatg gccagatgga aaaagcattg
    13921 gtatagatat gaatgcaaat aaaatacttg agtatgcaaa attatttggg cttgaccaaa
    13981 atacagggct tcaagatgaa gtagaagaaa gagcaggtaa agttcctagc acagaagata
    14041 aattgaaatc aacacaagct cttctaaagt caaatttaga aagagaaatg gcaaatgatt
    14101 ttgttgatat aactagagaa aaaaatccta aagaatatga gaaaagaatc aatgagattg
    14161 taagctgggc agcagaaaag aaaactcctg gtagagttga aacaatgaat aggctaaaaa
    14221 aattggatgt taaagaagat agaattgaag aagtagcaga tttagcagta tttagttatt
    14281 ttaactttgc aaaatggagt actgcagata catttaactt agctataggt caaggtgaaa
    14341 atgcttatac accagctcaa atatcaagat atgtagctgc tatagcaaat ggaggtaatt
    14401 tagtagaatt atctgtagtt gatagagctg tttcaagtga ttattcatca gttaaaatta
    14461 atgaccagaa gaaagtcgaa aaaatacctt ttaaaaatcc agataactta aaagaattaa
    14521 caaaaggtat gaaattggtt gcaagacaag gtacagcgaa gagtgctttt gctgattttc
    14581 caatagatgt agcagcaaaa actggtacag ctgagaaaag tgggaaaata cctacagata
    14641 acgagtatga atatttaaaa agtcatatgt catcttataa tgtcaaccta aatgatgcga
    14701 ttaaactagc tgataagatg aaagcagaaa aagaaaaaga gctttcatta gctaaggaaa
    14761 aggaaataaa aaagaaatta gaaaataaag atttgaaaga cgaagaaaga aagaaattag
    14821 aggaagaatt agaagatgga gtaaaagtta gattagagga cactgataag gtgaattctt
    14881 catatctaag aaaagctata aaagaattaa atccaaaaat aacagatgac caaatagata
    14941 gatttaaaca agattatggt tcttttacgt ggactgttgc ttttgctcca gcagatgacc
    15001 cagaaatagc tgttgtatgt gtaattcctc aaggtgattc aagtgtattc tcacttttac
    15061 caactagaga agttattgga acttatatgg gattagagcc aactaatagt aaaaatgaca
    15121 acaagacgaa tgatgttaac aattcttctg acgaaaatat aaatttcgag tcacaaatta
    15181 atagatagct caatctatgt ataaacttta gctttatata gaaaaaataa aagggtttaa
    15241 agcgtatttg aagaatataa atgatatatc actctggagg ttgttatgtc tttaagagaa
    15301 atttgtagtc aagaactagt agagttcaag ggtaataaaa gagggataat agtaaatata
    15361 aaaagagaag ctccatttga ggaaatacag gaaaaaataa taaacaaatt ggaagcatat
    15421 gttggctttt ttaatggggc taaaattagt aaaattaata gtgattgttt aactgacatg
    15481 gagattctag aacttaaaga aggaattact tctagatttg atgtggaatt tgttgaagat
    15541 caaaaaattg aagaaaatag taattttcca acaaaatatg tcaataccct aagatctggt
    15601 gaaaatattg aatttgaagg tgatgtagtt atattgaatg atatgaaacc tggttcaaaa
    15661 gtcctttcaa aatcaaatac agttgttatg ggagatataa atgctggtgc taaggttgta
    15721 gcaggaggta atgtttttgt aatgggtaaa atagaaggtt ttgtacatgc aggtgcagaa
    15781 ggcaatgaat ttgcatatgt tgttgctgga aatcttaatc ctaaaatatt acaaatagca
    15841 gataatattg cagaagctcc agatgatgaa gaaaattatg aaagtgaatc agaaattagc
    15901 ccagaaatag cctttgtaag taatggaaga attgtaatcg aaagctattt gtcaaagtta
    15961 gacaaataga aagaaaaata gggggtagta ttatgagcga agttatagtt ataacatctg
    16021 gtaaaggtgg ggttggaaaa actactaccg cagcaaactt aggaactgcg ctaagcctag
    16081 aaaataaaaa aacagtagtt gtggatgcag atattggact tagaaactta gatgtagtaa
    16141 tgggtcttga aaatcgaata gtttatgaca tagtagatgt agttgaagga acttgtaggc
    16201 ttaagcaagc tctaataaaa gacaaaagat ttgataatct atatttatta ccagcagcac
    16261 aaactagaga taaaaatgct gtctcagttg agcagatgat tgacctgtgt gagaaactaa
    16321 aagaatcttt cgagtatata ataatagatt gtccagcagg tattgagcaa ggctttaaaa
    16381 atgcagtagc tggggcagat agagctattg tagttactaa tccagaaata tcagcagtaa
    16441 gagatgctga tagaataata ggtctattag aagcaaatga aataaaagag ataagattag
    16501 ttataaatag aattagaaat gatatggtta agcgtggaga catgatggat aaacaagata
    16561 taatagaaat attagcaata gatttgttag gtcttgttcc tgatgatgaa agcataatta
    16621 tatcaacaaa taaaggagag ccagctatac ttgattctaa gtcacttgct ggtcaagcat
    16681 acaaaaatat cgcaaaaagg atactaaatg aagaagttcc tctacttgat cttgaagttg
    16741 aagatggatt ctttggtaga cttaaaaaaa tgtttagcat ggctaagtag caaaggagga
    16801 tacatctgtg ttagatttat ttagagtttt ttctaacgag gctaaaacta gtaaatctgt
    16861 tgctaaagag aggttaaagc tagttttagt tcatgataga gtagattgtt caccacaact
    16921 tttagagatg ataaaaacag acattttgaa agtaatagct aattatgcag aaatagaaga
    16981 tgatggtctt gaaataaaaa tgtctaaatg cagaggtgag catgatgaca aacctgtatc
    17041 tgcattagtt gctaatatac cactaaagaa tataaaagat agatgtatgt aattagtgct
    17101 taaaaggggg atatccttat tggtatatgc cccttttatt taattatatg tttaaagata
    17161 gaaagacggt gattctttga aaaaaatcaa tattaattat aagtcgacaa ttaagttgat
    17221 aaaacagtta gactggaaac ttatagttac agtattagct atctttattt ttgggcttgt
    17281 tatacttagt agtgcaacac atgctaattc aacaggttca tataatcaat tgattaaaca
    17341 aggattagct tttgtacttg gtataggaat gattatagtc atattatttt ttgactataa
    17401 ccttctaggc aggtattata aagcgcttta tataataagt cttattttgt tagccatagt
    17461 tttattacct ggaattggga cagtaaaagg tggagctagg tcgtggatta acttaggacc
    17521 acttgacttg cagacatctg aaatagtcaa attaacattt gtactaagtt atgcaaagat
    17581 acttgaatcc aaaaaggata aattaaatac gttaaaggaa gttatgcctg ttgtagttta
    17641 ttctcttcca tttatcggat tattgattgc acaaccagac ttgggaacag gaattgtatt
    17701 ttgttgtatg atatttgcaa tgctgttcac agcaggtctt agttcaaagt tgataaaaag
    17761 aggaataatc atactattag tgtcaatgcc actgatgtat ttaatgatgg cagaccatca
    17821 aaaggtaaga atagaagctt ttctaaatcc agaagatgta actctaaaag gaaactatca
    17881 agttatgcaa tctctgattg caattggttc tggaggcgta actggtaaag gactttacaa
    17941 tggaagtcaa aatcaagaag attttttacc tgtacaagat agtgatttta tatttgctgt
    18001 tgtaggtgaa gaacttggtg taataggaat ggcagtgttg ataatcttat ttatgatatt
    18061 cctattaagg cttttagcaa ttgcaagaga tgcaaaagat ttttatggaa ccctcatagt
    18121 ggttggtgtt atgggaatgt ttggttacca gataatccaa aatataggta tgacggttgc
    18181 gttaatacca gtaactggag taacattacc ttttgtaagt tatggaggaa gttcgttatt
    18241 gacatcttta gcaaacttag gattagtact aaatgtgtgt atgagaagaa agaaaataaa
    18301 tttctaaatt taaatacatc taaacataaa ttgaggagat tagatatatg aatatagcat
    18361 tagtagcaca tgaccaaatg aaaaacacta tggtcggatt ttgtataggt tatgaatcaa
    18421 ttttaaaaaa gtatggacta tatgcaactg gaactacagg aaaaagaata atggatgaaa
    18481 ctgaattaaa tataaataga ttagcatctg gtcctttagg tggagaccag caaataggtt
    18541 ctttaatagt aacacaagaa atagatttag ttattttttt aagagaccca ttgacatctc
    18601 aggctcatga aacagatata caagcgttaa taagactttg tgatgtttat catgttccaa
    18661 tagctacaaa tctagcttca gcagaaatat ttataaaagc tcttgataga ggagaactat
    18721 catggagaga agttagaaaa agcaaaagtc aacgtattta aaaaatatta atattacagt
    18781 ctaaagaaac agatatataa gtgtttaatg ccctagtttt tctgttggaa taaatctagt
    18841 acctgtagaa atcttatgaa agatttaaat gatgaagagc taaatttgag attaaataat
    18901 taggaaatag attatagata gagtagtaga atttaaacta ttactctatt ttaataatat
    18961 gaaatagaat caagtctatt attatatata tttaaactta taggtaatac tatttcaacc
    19021 aagtaaatgt tgttatgaca acattaataa aacaaaaatt tatttttaaa atttgataaa
    19081 tagctgtata ttagtattta tagattaaat attaatatac agctatttat tatttttaag
    19141 tctattgtag aatataaacc ttgtattata gagtactatg taatattata gtaaatataa
    19201 gtactataca ttatagagta gtaggtgaaa aggcggtggt acataatgca gttaaataaa
    19261 gaagtgttaa aaggttatat cgatatatta attgtttcta tattagaaaa gaaagattgt
    19321 tatggatatg aaattgcaaa gcaagtaaga gaaagaagtg agtttgaatt aaaagaagga
    19381 acaatgtatc ttgcacttaa aagaatggaa tctaaaaatt taattaaatc ttattatagc
    19441 aatgaacaaa gttcaggtgg aagaagaaaa tattataatt taactaatga aggtaaagat
    19501 tttttagaaa taaagaaaca agaatggaga tttattaaaa aggttatgaa tcaatttttg
    19561 ggagaggtgg attaatttat gaaactaata gatgaatatt tagataaact atataaaaaa
    19621 tgtgacaata aaagtactat agaattaaaa caggaaatga gatgccatct tatagaatct
    19681 gcaaatgaat ttaaattaga aggattagat gaagaagaag catgtaaaaa agcaattgaa
    19741 agatttgatg atggtgatga aatgcagtat gaattatgta atataataaa agaactatct
    19801 ttatcattgg ataggcataa atcaattgta atggggttta aaaaagtatt aggttacata
    19861 agtataattg catttttaat ttctggattt atgtggtatt acaataatag tttacaacat
    19921 aatatgtata atttaggaaa agaacttgat ggagaaataa agcaattggc agaaagacat
    19981 gatatgacaa agattggtga atataagtta gaacttgaaa aaatacttga taaagataag
    20041 tatagtaaag tcaaagctct tagactatat gtaatagata tgaaagatgg aaacactaat
    20101 ttaagttctt ctggattgaa tgctaatatg gtatatgaga gggaagcaga ttataataac
    20161 ataagtaatt ttatacagca tttaggatat aatggaaaag attttttaga caagaatggg
    20221 aatatagtaa atccagatat tttcttagaa tatttctttt attttgaatc ggagatgtta
    20281 attccagtag cattcgtatt tggattatta tgtataatag cgtattttat attaagattt
    20341 aaaatttctt taataaaaaa taataattaa ataattcacc aaataatttt aatttgtaga
    20401 gtaaataaga tgtttcaaaa taaaatttga atcatcttat tttttattgt ttgttatatt
    20461 cttttctttt atatttctcc atagtggatt gtatctatac ataatcacat taaaaacaac
    20521 acaacatatg ttaactggaa cgcagcagag ggagccaata ctgcttatac attggttact
    20581 aaaaaaggtg cttgttctgg aatgtgtagg agcttgaaag ctttatgtga tgctatggga
    20641 attgaatgtt attatgtaca tagtacaaca aatgaccatc agtggaatct tattcgtttt
    20701 gaggatggac gtttgtatca tgtagatatt gatgccaaca aatcagcagg agcagacatc
    20761 atatacaaga gtcttactca cccattacca tttgacaaga aagcatatcc agctgttggc
    20821 gaagtagtaa agacacagaa tccaacgcca actgttaaga aacctacgcc agctaagcct
    20881 tctgcgccag ttggacctgg gaatcacact atgatagcag atcaacaagg ggcaatactt
    20941 gatgaagttt atggaaataa tctacgtcca aaaattgaaa tgtatgaggg agttccgtgt
    21001 agtttaatca actcatcaat aaatacaatt aagtttacaa gcaaaaattc aaatttagac
    21061 ccatcaaagc atacagttac tgtaataggg tttatgaata caccatatgg tatggttgca
    21121 ttagcaccag agagcaaacc tgttagttat ccttttgagt atgataaggc attttcttta
    21181 gattttactg gtaagaacct ttctgcagca gaattatcaa aaaagtgtaa aaaaataata
    21241 taattattaa agtagaaatt ggtgaaacaa catctaattc tggaataggg ctagtagcat
    21301 atactagata cgtgaattaa tacaagtata ctagtataca aatagaacag actataaata
    21361 atgcaccctg tgcgaaaggg tacatttatt attatattag tcattttgga aaataaaaaa
    21421 ggagttgaac ttaaaattag attagaaaaa ttaattttga gacaactcca ttttaattta
    21481 tttttaaata taaaatcatt tattttaaat aatattatgt ttgattaatg gctatttaac
    21541 taattttgca aattctgcac caaatgccat acactctttt aatgtctctt cactagggaa
    21601 gaattttttc ttaagagatt ctacaggcat tttgaatgac atagatttaa gtaaagtctc
    21661 agccatagtt ataccttcac cactccaacc aaatgaacca aatactcctg ctatcttgcc
    21721 ttggttagcc attgggtcta taacagagaa taaatcccac ataggtttaa ccatagtttt
    21781 gtttatagtt ggagagccta gtaatattac ttttgacact actaatgtat cgtgcatttc
    21841 tgttaatgtc atatcttcta aatcatataa ttcagccttt gcaccttctt tatcaagtcc
    21901 ttcttttatc tttttagcca tctctaaagt attactataa gctgataagt agaatataga
    21961 aacttgattt tgattagttg tattaacagc ttctgtagac cactcaacat atcttttaac
    22021 agcagccatt gggtctttag taagcattgg accatgagaa gtcaatatag tatcaaattc
    22081 tatgtttagt cctaccacct tatctacagc acttaaaaca tgtttagcaa atggcttaac
    22141 tatacaatca tagtagtgtt tagctgattt tagataatct tcactattca ctacctctgc
    22201 atcaacactt gcaaagtgac aaccaaatgc atcacaagtt aaaagagtct tgtcctcctc
    22261 aatgtaagta aacattgtat caacccaatg taagaatgga gcagttataa atctaagatt
    22321 tcttttaccg atatttaaaa tttcaccatc ttttattaca tgacagttaa aaggtctatt
    22381 tatttgacca tctaagtata gtttagcagc tttagtacaa taaacttcta tattaggatt
    22441 tatatctaat aagtatttta aactacctgc atggtctggt tctgtgtgat gaattacaac
    22501 atagtcaatt ttagctatat cagtaacctc tgataaatta cttaaaaact catctttaaa
    22561 atttgccttt acagtgtcaa ataaaaccgt tttttcgtcc ttgattaaat aagagttgta
    22621 agttgtccca aactcagttt ccattattat gtcgaatact tttaaacctt catctactac
    22681 tccagtaaag tagatatctt tttttacttc aaaaacttta ctcattttta accctcctaa
    22741 aatttatcat tatataagta acatacccca ttttgaggtg aatgaaacaa aagatgtcat
    22801 gataagtcat gtaaaacaat agttt
//
LOCUS       DAYASV010000055        22275 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYASV010000055 DAYASV010000000
VERSION     DAYASV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.055"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            288..746
                     /locus_tag="ACGI68_002048"
     CDS             288..746
                     /locus_tag="ACGI68_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138485.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            807..1082
                     /locus_tag="ACGI68_002049"
     CDS             807..1082
                     /locus_tag="ACGI68_002049"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138486.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1141..2415
                     /locus_tag="ACGI68_002050"
     CDS             1141..2415
                     /locus_tag="ACGI68_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFH0138487.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2545..3597
                     /locus_tag="ACGI68_002051"
     CDS             2545..3597
                     /locus_tag="ACGI68_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0138488.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3597..4628
                     /locus_tag="ACGI68_002052"
     CDS             3597..4628
                     /locus_tag="ACGI68_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFH0138489.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4720..5532
                     /locus_tag="ACGI68_002053"
     CDS             4720..5532
                     /locus_tag="ACGI68_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0138490.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5535..6455
                     /locus_tag="ACGI68_002054"
     CDS             5535..6455
                     /locus_tag="ACGI68_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HFH0138491.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6630..7076
                     /gene="rpiB"
                     /locus_tag="ACGI68_002055"
     CDS             6630..7076
                     /gene="rpiB"
                     /locus_tag="ACGI68_002055"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HFH0138492.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7106..7771
                     /locus_tag="ACGI68_002056"
     CDS             7106..7771
                     /locus_tag="ACGI68_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0138493.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7881..9383
                     /locus_tag="ACGI68_002057"
     CDS             7881..9383
                     /locus_tag="ACGI68_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFH0138494.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRPYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9695..11287)
                     /locus_tag="ACGI68_002058"
     CDS             complement(9695..11287)
                     /locus_tag="ACGI68_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HFH0138495.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLVSILFEAVYDLNNNFSYN
                     INYYYKEFALPYFYFFGTPVHLSKVELEFVCIGIILSSFLLTILCFTLYKKNKQTKVK
                     FLENVISLFSKVKIELTLILTLILLTLRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLNMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEV
                     IGDDEISTLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDRETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELEN
                     AHMEIIIDADLFKVMLTFRRVN"
     gene            complement(11256..11972)
                     /locus_tag="ACGI68_002059"
     CDS             complement(11256..11972)
                     /locus_tag="ACGI68_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138496.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(12512..13312)
                     /locus_tag="ACGI68_002060"
     CDS             complement(12512..13312)
                     /locus_tag="ACGI68_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0138497.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            complement(13626..14357)
                     /locus_tag="ACGI68_002061"
     CDS             complement(13626..14357)
                     /locus_tag="ACGI68_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HFH0138498.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(14344..15039)
                     /locus_tag="ACGI68_002062"
     CDS             complement(14344..15039)
                     /locus_tag="ACGI68_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0138499.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            complement(15041..15832)
                     /locus_tag="ACGI68_002063"
     CDS             complement(15041..15832)
                     /locus_tag="ACGI68_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138500.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAIILSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQVFVNLPLTIKILYTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(15842..16654)
                     /gene="modA"
                     /locus_tag="ACGI68_002064"
     CDS             complement(15842..16654)
                     /gene="modA"
                     /locus_tag="ACGI68_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0138501.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            complement(17288..17488)
                     /locus_tag="ACGI68_002065"
     CDS             complement(17288..17488)
                     /locus_tag="ACGI68_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0138502.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(17713..17889)
                     /locus_tag="ACGI68_002066"
     CDS             complement(17713..17889)
                     /locus_tag="ACGI68_002066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138503.1"
                     /translation="MFRDKMDKCTHMLTAYISNSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(17943..18032)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(18212..18412)
                     /locus_tag="ACGI68_002067"
     CDS             complement(18212..18412)
                     /locus_tag="ACGI68_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138504.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(18414..18764)
                     /locus_tag="ACGI68_002068"
     CDS             complement(18414..18764)
                     /locus_tag="ACGI68_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HFH0138505.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            18926..19114
                     /locus_tag="ACGI68_002069"
     CDS             18926..19114
                     /locus_tag="ACGI68_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138506.1"
                     /translation="MTKYDFNFKLKVAKFYLNREGRDILIVKQYGVYDHSQVERWVNI
                     LGESELKIKKFSILYGSS"
     gene            complement(19591..19791)
                     /locus_tag="ACGI68_002070"
                     /pseudo
     CDS             complement(19591..19791)
                     /locus_tag="ACGI68_002070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04223.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20150..20638)
                     /locus_tag="ACGI68_002071"
     CDS             complement(20150..20638)
                     /locus_tag="ACGI68_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HFH0138507.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTEGNIIWWTKKDGKITVNSGIGDEDPLAHKIENLGMPYN
                     GSYTLVSSNGSEEYLELNIIIDGEISKSGLDKLEEDYGSSIKIPNDKNMIITFLSNKS
                     DN"
     gene            21442..21816
                     /locus_tag="ACGI68_002072"
     CDS             21442..21816
                     /locus_tag="ACGI68_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0138508.1"
                     /translation="MKEEILIEKLLRAELIVMEYLWKKDTLMPKKEIVKEIKQIYGWH
                     KSTIKILLKRLVAKRYLARYIINSQSHYKIIDSKEYYTFKKKVLKSSKSIKIMRSLTT
                     THKNVSKEKLDLLDEYYRNLKE"
     gene            21921..22275
                     /locus_tag="ACGI68_002073"
                     /pseudo
     CDS             21921..22275
                     /locus_tag="ACGI68_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         8607 a         2716 c         3319 g         7633 t
ORIGIN      
        1 acataaaaaa ccatttgtaa aaaacaaaaa aatcatttct ctaacaaaaa aaaccacaaa
       61 aaatataaaa aagtatggct agttaagtga ttaacaagta aaaattaaaa taaaaatcaa
      121 aaaaaattta tcaatgaaag tcagctaaaa tcctggaaga gagcattatt tttgggaaaa
      181 cattttcttt tgtttcacaa aaaaagccac tttttggcac acaacatgct ttatataaag
      241 tcaagaaaaa ctaaggtata agacaaaggc aattaggagg tgtaaatatg gagtttataa
      301 attttgtaag tgataaagtt atagacctta aaatggattg taaaacgaaa gaagatttct
      361 ttaaagaaat acataataaa gtttttgatt taggctttgt aaaagaagaa tttggagaaa
      421 aaatattagc aagagaaaat gtatttccaa caggtttaaa tttaggtgat tatggagtag
      481 caataccaca tacagatgcg gaatatatta aagagcaatt tatatcagtc tgtactttta
      541 atgaaccagt agtatttagt tctatggaag accaagatga aaaagtacct gtaaatttgg
      601 ctttcgtatt aggacttaat caaccacaca gtcaattatc tgtattaaca gaattaatgg
      661 gaattatgca aaataaagag ttagttgata agttgatgag ttctactgat aaagaagaag
      721 ttttaaagac actaaagtct ttataaaaat aaaaaagtac taaaaataaa aaatataaaa
      781 ataaaaatta ttaggaggaa ataaaaatga aaaaaatatt agtagcatgt ggagcaggaa
      841 tagcaacatc aacagtagtt tgtgacaagg tagagagatt agttaaggaa aataatatac
      901 aagcagaagt tatacaatgc aaaatagctg aatgtgcaac aaagcaagaa ggagcagatt
      961 taatagtatc aacaactata ctaccaacaa cttataatat accagcaata aaagcaacag
     1021 catatataac tggagtaaat acagcagcat tagataaaaa aatattagat gctttaaaat
     1081 aatcctatca tagatacatt gaaataatat tttataaaac agaaaaagga ggatttataa
     1141 atggataaat tattagcagt agtacaatac attttaaata tgggaccgac agtaatacta
     1201 ccaataacaa ttgtagtact aggaatgata tttagaatta agtttacaaa agctttaaag
     1261 tcaggtctta caattggtat aggatttgtg ggtataaact tagttgtaaa cctattaaca
     1321 tcaacactag ggccagcggc tcaaggaatg gttgaaagat tcggacttaa tttaacagta
     1381 atagatgttg gttggccaac aacagcatca gcaacatggg cttcaccagt tgcaccaata
     1441 ttaataccag tttgtttatt agtaaactta ctattattat acttcaataa aacaaagata
     1501 ttaaacgttg atatatggaa ttattggcac tttatagtag caggtgcagt tggatatata
     1561 gtaacaggaa gttatgtttg ggctatattc tgtgcaatac taatggagtt attagtactg
     1621 tttattgcag aaaagacagc aaaaaatgtt caggagtttt atgatttaga aggtatagca
     1681 ttaccaactg gttcaacagc ttcatttgct ccacttggat atttagtagg taaatttgtt
     1741 gaaaaaatac caggaataaa taaaatacaa gcagacccag actctataca aaaaagattt
     1801 ggtatatttg gagaaccaat gatgatgggg attatactag gtataatact tggagcactt
     1861 gcgggatatg atgtagcagg aatattcaat gtaggtatat caatgggtgc agtaatgtta
     1921 cttatgccta gaatggttag aatattaatg gaaggtttaa ttccaatttc agaatcaata
     1981 agagatttct tacaagcaaa atatggagac agagaacttt atataggtct ggatgcagca
     2041 gtagcaactg gacatccagc agttatatca acagctctta tacttgttcc tataactttg
     2101 ttcttagcag ttatactacc aggaaacaaa gttttaccat ttggagactt agcgacaata
     2161 ccattttata tggcatttat agtgtgtttt agaaaaggta atatagttca atcagttata
     2221 acaggaacaa tagtaatagc tatatcactt tatatggcta caaactttgc agatgttcat
     2281 acacaattat tacaacaagc aaatatgatg ccagaaggaa caacaatggt tacaagtata
     2341 gatacaggtg gaaacttgct aaaatgggga atattgaaat tatctcaatt agtttcttca
     2401 tttggaatat tctagtatgt agttaataga gattactctt ttttagtaaa gtatgcgaaa
     2461 atttaacatt tagcagctac taaaaaagag taatattttt tataaatttt taaaaataca
     2521 aaatacaaga ggaggagatt tatcgtgaaa gcagcagtat tacatggcac aaatgatatg
     2581 agatttgaag atatagaaat aaaaccatgt gaaagtgatg aagtaaaaat aaaagttatg
     2641 gcagcaggaa tatgtgggtc agatcctcca agagtattaa aacattggaa atatccagtt
     2701 ccagctatac caggacatga gttttctgga gtaattgctg aagttggtaa ggatgtaaaa
     2761 aatgtaaaag ttggagatag agtagttgca attcctttta taccttgtaa tgaatgtgaa
     2821 tattgtaaaa gaggattatt ttcattgtgt gatgaccatg gaatgttagg agctaaaagt
     2881 tttggagctt ttgcagagta tgtaaatata aaagctacaa atgtacttcc aataggagat
     2941 atgaactttg aagatgctgc aatgatagag ccactagcag ttgctatgca tggagtactt
     3001 aatataggtg ttcaagttgg agacactgta gctgtaatgg gtagtggaac aatggggcag
     3061 ttggtaatac aaggacttaa aatagcaggg gcaggaacaa ttatagcagt tgatatatct
     3121 gataataaat taagagaatc aaaggaatta ggggctgata taataattaa tgctaaagat
     3181 attaacccag tagaaaaaat aaaagaactt actggtggaa aaggtgttga catagctctt
     3241 gaatgtgcag gttcaaaaat aactcaagaa caatgtttac ttataactaa aaagaaaagt
     3301 aagataggat ttttagggat agcatattca gatataactt tatcagaaga agcatttgaa
     3361 aatatattta gaaaagaact agaattaaaa ggtttttgga actcttattc tgcaccattt
     3421 ccaggacaag aatggactaa gggaatcaat ctagttaatg aaggaaagat aaaattaaaa
     3481 gaaatggtat ctcatagatt ctcacttgaa gatacttaca aagcatttga aatgataaga
     3541 gatagaaaag aagaatttaa caaaatatta attttaccac aaggggttga aaaataatga
     3601 aagcagtagt taaaactaaa ccaggctatg ataatgtaga agttttagaa gtagaagaac
     3661 caaaagctac aggagacaag gttaaaataa aagtagaata tagtggtata tgtggttcag
     3721 atatacattc atttaaaggt gaatatgcaa atataaaagc cccagtaaca ttaggtcatg
     3781 aattttctgg aattgttgta gaagtaggag aagatgttaa aaatataaaa gtaggagata
     3841 gagttacatc agagactact tttgcaactt gtgagaaatg tatatattgt gaaactaaag
     3901 attataactt atgcccaact agaaaaggta ttggtactca agtaaatgga agttttgctg
     3961 agtatgtatt atcaagagaa gaaagcatac atgtacttcc tgaaaatgta tctttattat
     4021 cagcagccct tacagagcca ctagcttgtt gtgttcatgc agctcttgaa aagactacta
     4081 tagaaagttc tgatacagtt ttaataatag gaccaggacc aattggttta ttattagctc
     4141 aagttgttaa atctcaaggt gctacagtta taatgtctgg tgttacaaaa gataaggaaa
     4201 gattagaaat agctaaaaat cttggagtag atagaactgt aaatgtaatg gaagaaagtt
     4261 tggcagatgt agtgaatgaa atgactgatg gaaatggagt aaacaaagga tttgattgtt
     4321 ctgggtttat gccagcagtt aacgaagcct taagactgct tagaaaaaaa ggtactttcg
     4381 tacaagttgg aatctttgct aaaaaattaa atgaaatgga ccaagaagca ataatccaaa
     4441 gagagttaca atatatagga agcaggtctc aaaagccaag ttcttggatt acagcattag
     4501 atttaatggc aaatgggaaa gtaaatacag aagctttagt tactagaact gttggtttag
     4561 acgaatttaa agaaggtatt acagctttaa tgaacgggga agaaataaaa gtagcagtaa
     4621 aatcttaaaa aaacatttgt attattttaa aaaaaattgt aaaatctact gtaggataaa
     4681 aaaatgtaag gtcttaaaaa ataacaggag gaagaaagta tgagcactct tagagatcat
     4741 tctaataata ttagaaaaaa cataataaaa atggttgcag aagctaaaag cggacatcca
     4801 ggtggttcat tatcaatagc tgatatgttg acagtattat attttgataa gatgaacgta
     4861 tgtgcagaaa atccaaaaat ggctgataga gatagatttg ttctatcaaa aggtcatgca
     4921 gcaccagctt tatatgctac attagcagaa aaaggatttt tcccagaaga agaattaata
     4981 acactaagaa aatttggttc taagttacaa ggtcacccag acatgaaaaa agtagcagga
     5041 atcgatatgt ctactggttc tcttggtcaa gggctatctg cagcaaatgg tatggcactt
     5101 gcaggaaaat tagataacaa agactataca gtatatacta tattaggtga tggagaaatc
     5161 caagaaggtc aaatatggga agctgcaatg acagcatcac attacaaatt agataattta
     5221 attgcatttg ttgacttaaa tggattacaa atagatggtt ctaatgaaga agtaatgaat
     5281 gtaagtccag tagatgacaa attcaaatct tttggatgga atgtaatagt aataaatgga
     5341 cattcttttg aagaaatagg aaatgcagtt gatgaagcta aaaaagttac aggaaaacca
     5401 actgtaataa tagctaaaac agtaaaaggt aaaggtgttt catttatgga aaataatgct
     5461 gcatggcatg gaacagctcc aaatgcagaa caagcagagc aagcattgaa agatttagaa
     5521 ggaggtctat aggaatggga atagcaacta gagaagctta cggacaagtg ttaaaagaac
     5581 ttgcagaaaa taaagatata gtagtattag atgcagattt aggaaaagct acaaaaagta
     5641 tatcatttaa agaagttgcc ccagatagat tttttgacat ggggatagca gaaggtgaca
     5701 tgattggtac agcagcaggt cttgctactt gtggaaaaat accttttgcg agtacttttg
     5761 caatatttgc agcaggaaga ggctatgagc aaataagaaa ctctgtagca tatccaaatt
     5821 taaatgtaaa aatagcagct actcatgcag gtgtaacagt aggtgaagat ggtggtagcc
     5881 atcaagctat agaggatata tctctaatga gaggtatacc aaatatggtt gtattaaatc
     5941 cagcagatgc tttagaagca agacaagcta ttcttacatc tattgattat aatggacctg
     6001 tttatataag attaggtaga gctgcaactc ctgatgtaaa cagtgaaaat tataaatttg
     6061 aaataggaaa aggaactgtt ttaagagaag gttctgacat aacagtaata gctacaggaa
     6121 taatggtagc aaaagcctta gaagcagcag aagaacttgc taaagaaggt ataaatgtag
     6181 aagttgttaa tatatctaca ataaaaccat tagatgaagc attaataaaa gaaactgcta
     6241 agaaaactgg aaaagtagtt acagcagagg agcacagtat aataggtggt ttaggttcag
     6301 ctgtatgtga agctttagca gaaactaaag atgtagtagt tagaagaatt ggtgttaaag
     6361 atgtatttgg tcaatcaggt acaccagctg atttattaaa acactatggt ttaactacag
     6421 aagatatagt aaaaaatata aaagagttgt tatagatata aatatatcta aataacaaat
     6481 tgatttgaca taagtcaatt aaagctgtct taaaaataga aagaaatttc tacaatggga
     6541 cagctttatt tttacaatac tttattttta tgataattat aaaatagtct gaaagtaatc
     6601 aaaaaaatta taaaaagagg tgctatttaa tgaaaatatc tattggatgt gaccatggtg
     6661 gttatgaatt aaaggaatat ataaaggaaa atttaataaa taaaggtata gaagttgaag
     6721 atgtgggtac aaattcaaaa gaaagtgtag attttccaat atatgcaaaa aaggtaagtc
     6781 aaaatgttca aagtaaaaaa tctgatttag gcatactttg ttgtggaaca ggaataggta
     6841 tgtctattgc agcaaataag tttaaaggaa tcagagcagc tgttgtttct gactgttttt
     6901 cagctaaggc aactagagaa cacaataata caaatgtact ttgtttagga gaaagagtta
     6961 ttgggaaagg attagcaatg agaattgttg aagaatggtt aaattcaagt tataatggag
     7021 aaagacatgc aagaagatta caaatgattg atgatataga aaaagaaaat ttataaatat
     7081 ataatttata acaaggggga gcgttatgtc tattatttgt gcttcaataa tgtgtgcaga
     7141 ccaaatgaag ttaaaagagg aattagaagc tcttgaagaa gcaggtgtaa aattacttca
     7201 ttgtgatgtt atggatggaa tatttgttaa aaacttggct atgggaccag agttattaaa
     7261 atcaataagt gaaaatacaa caataccttt agacatacac ttagccacag aaactccaga
     7321 taaatatatt gatatgatgt cttatataaa gccaaaatat atttcttttc atgtagaatc
     7381 aagtacaaat gtaaaagctg atatacaaaa gttaagaaat tatggtatag ggccagtgtt
     7441 agcaataagc cctcaaacaa gtgtggataa aatagaagaa tatataagtt tagttgaggg
     7501 tatcttagta atgacagtaa atccagggtt tgcaggtcaa aagtttaatc tttctgtatt
     7561 ggataagttg gataaactta cagagatact aaaagattat gataatcctc catttataga
     7621 ggttgatgga aatataaata aggatactat taaacttatg aatggtaaaa aagtagatat
     7681 atatgttgtt ggtacttcag ctttatttaa tgataagcct ccaatttcat ataaagataa
     7741 aatagaagaa ctaaaagaaa gtataaagta accatgtaat aaaattataa tatgtttctg
     7801 tttaatagta aaaatttagg tggaaacaag aaattttata atatttaata aaaattttgg
     7861 ttgagtaagg agaatttttt atgggagacc tatataaact acaaaatggt actgatatta
     7921 gaggaatagc gtatgaaaat gattctaaag aagttaacct aactgtagaa gaagtaaaaa
     7981 aaatagctaa agcgtttcat atttggttaa aagaaaagac taagaaagat aaagtaactg
     8041 ttgccatagg aactgattct aggataacag gaagccaatt tagaagtact gttattgaaa
     8101 ctttaactaa tgacgattgt aatgtaattg attgtgagat tgccacaact ccagctatgt
     8161 ttatgacaac tattatggat ggatacaatt ctgatggggc gattatgatt acagcaagtc
     8221 acttacctta ttactataat ggtttaaagt ttttcacaga aagtggaggt ttagaaaaaa
     8281 ctgatataaa agagatgttg gatatagctg ttaaaaatga tagtgtagac tatgaggaag
     8341 taaataaaaa aggtgaagtt atagtaaaaa atttaataga agattattct aatttattga
     8401 tagataaaat tagaaaaggt gtaaattcaa gtaaaaatta tgaaaagcct ttttctgggc
     8461 ttaagatatt ggttgatgca ggaaatgggg ctggtggatt ctttgctgaa aaagttttac
     8521 acatactggg agcagatacg acaggtagtc agtttttgaa cccagatggt atgttcccaa
     8581 atcatatacc taatcccgaa aacaaagaag caatggaaag catatgtaaa gctgtgttag
     8641 ataataagag cgacctagga ataatctttg atacagatgt agacagagct gctattgtag
     8701 gtaaaaatgg caaaccaata aataaaaatg ctcttatagc tgttatatca tcaatagtat
     8761 tagaagaaca tccaaaaaca gctatagtaa cagattcgat tacttctgaa ggacttgcta
     8821 aatttataaa tgaattacaa ggaagacatc atagatttaa aagaggatat aaaaatgtaa
     8881 taaatgaagc tataagattg aatagtgaag gtgaagagtg ccatttagct atagaaacgt
     8941 ctggacatgc tgctttaaaa gaaaattatt tcttagatga tggggcatat ttaatagcta
     9001 aaattttaat aaaagtagca aagctatcct tggaaggaaa gactatagaa gaattgatag
     9061 aaaaccttga agaagctcaa gaagagaaag aagttagaat aggcattaat aaagaagatt
     9121 ttagaccata tgcagaggat atattaaaag atttagattc tcatgtcaag agtattgatg
     9181 gatggtctgt agcaccaaat aattttgaag gcataagagt taattgtgat gaatcaaatg
     9241 gagatggatg gtttttactt agaatatcac tacatgaacc attattagct ttaaatattg
     9301 aatcaaataa aattggcgga gcagaacaaa tttattctaa gttaaaactt ttcttagaaa
     9361 aatatgattt aaaaggtctt tagggtaaat agaaagaata tatttatatt gaagaataaa
     9421 tgtaaatttg tacctaagat tacaaatatt acaaaattgt aatcttaggt aacagagatg
     9481 taactgatta tggaaacact atgtgatatt atagatacag agagatgaaa tatgtgatga
     9541 attatagaga aatataattt aaaatgtatt aatatcacac caaaaaggta taatcaaaaa
     9601 atatgtaaat ataaacccca acatttagaa gaagacctct aaggaggttt ttttcttgtt
     9661 taaataactt aaaatatatt tttatatcaa tatatcaatt aactcttcta aaagtaagca
     9721 ttaccttaaa taaatcagca tctataatta tttccatgtg tgcattttct aattcaatta
     9781 tattttgagc aattgataaa ccaagaccag agccatctgt ttttctagat atatcacccc
     9841 ttctaaatct ttctattaac tcttctggct ttagatttag tttatcattt gaaatatttt
     9901 taaacgttaa tattatattt tcttccttta taaacatatc tatgtagacc ctagtgttag
     9961 gcatagagta ttttagtata ttgcaaatta gattctcaaa aactctccaa gtttttttac
    10021 catctatcat ggtaaaaaca gcatattcag gtgtttctat tataaaatct aaactgctat
    10081 tttgaatttt gtcttcaaac tctacaatac tttgaattaa aagttctctt atatcggttt
    10141 ttgttatatt aaattctaca tctccactac ttagcttaga tgcttcaaat atgtcatcta
    10201 ttaataattt taatctttgt gattttttat ctaaaatatt tatatagtca tttatagtct
    10261 ctctgtcaaa gtcattattt tttaacatat caacatagct taatatagat gtaagtggag
    10321 tttttaagtc atgagatacg tttgtgataa gctctgcctt catcctttca ttttttatat
    10381 tagcatctac agcagtttca agaccacctg ttatattatt tatacttttg gctaaagtag
    10441 atatttcatc atctcctata acttctaatt tatactttaa attgccattt tctatatttt
    10501 ttatattttt tgatatgtta tttatgtaat taaacttaac agataaaaag ttgtatatat
    10561 aaaaatgaaa aactgtgttt acaagtgtaa ataaaattac tatagctatt gcctcagcat
    10621 aatatatata cataaagaat atcaaaacaa gatattggat aatagcttgt acaactatag
    10681 atattaaaaa tactatagct aattttttat ttatagtttt tttattatac atattaagca
    10741 agtttgaaaa aaatacatat gttatagaca tatcttttag attatagtta ttatatttaa
    10801 tattttcata tattatatag actatagata taattataaa attgtagatt attaaattag
    10861 tgttgtaaaa cattttatta cttgtaaatg catgaaacct taaggttaat agtatcaaag
    10921 ttaatataag tgtaagttct atttttacct tagaaaaaag gcttatgaca ttctctaaga
    10981 atttgacttt agtctgttta ttttttttat acaatgtaaa acatagtata gtcaataaaa
    11041 aacttgataa tataatacct atgcagacaa actctaattc tacttttgac aaatgtacag
    11101 gagtaccgaa aaaataaaaa taagggagag caaactcttt ataataataa ttaatattat
    11161 aagaaaagtt attattcaaa tcataaactg cttcaaataa tatagaaact aatagaaaca
    11221 gtaataataa taaaataaat tttgctttag aatcattaat aattaagttt ttcaattttg
    11281 taaccaatgc cccacaccac ctttaaatat tttggttctt ttggattaat ttcaatcttt
    11341 tctctaattc ttctaatatg aacagcaata gttctctcac aactataagc atcctcattc
    11401 cagacatttt cataaatctc atgagaagag aaaattttac cttttcgtga cataagaagt
    11461 gtaagtattt tatattcaag tggtgtcatt ttaatttctt caccatctaa agaaactatt
    11521 tttgtatctg tatttagctc taggccacca ctttttagaa tataattagt atcatttgag
    11581 ttatttgtat tactaaaatc acctaaattt atataccttc taagttgaga ttttactcga
    11641 gcagtaagtt ctagtggatt aaatggttta gttatatagt catcagcacc catattaaga
    11701 cctataattt tgtccatatc ttctgatttt gcagataaga gaattattgg tatattcctt
    11761 ttttctctta ttttaagtgt agttttaagc ccatctaaat tgggcatcat tacatccatt
    11821 attattaact taatatcatt ttcgattagt atgtccaatg cttcaagtcc atcatatgcc
    11881 tttaaaacat taaagttatc aggtctcaaa tagaactcta tagcatcaac tatctctcta
    11941 tcatcatcta caaccaatat attttgattc aacttattaa cccccttgaa attttatttt
    12001 taaccaattt ttattttata ccaaaaagag gtattagaaa acagtaagcc cgttcattat
    12061 gaaataaaaa ctagtacttt taatatattg tcaatgcata tagtaaattt aaaagtatag
    12121 acgcccatat taatctgaag gtaaaagttt atgaaaatat ctagggtaat ctatcacact
    12181 ccttatgtta ttttggttta ttaatttttt aggtttaaac atatattaac aaatataaat
    12241 aacaaatgag tttcataatt attaagaatt tataacaaat atttatttta atgtagttta
    12301 atataaatag gtagcttaat gtagatatgt ataaagcatt taataaggct tataggtttt
    12361 aatgatgata tctttatata ttttaagaga tgatgaaatt agatatgggt aatagttgta
    12421 tgagaaaaag tagtatagca caaaaagtgc tatatcacaa aatctcaaat aatttccttt
    12481 ttataactta aattaaaaga ataaatattt cttataaatt aggtgtatga agtatagcag
    12541 gaacatcttt attatttaat aacttatcaa atgatttatg gtcagactta tagccaatac
    12601 cttctctata ttctaattca tcaacaattt tgtattgttg acaggcattt ttaacctttt
    12661 catagtcttc agtagaatga gctttataaa ctatcttatt attaaaagtt attttatcag
    12721 caaatttagg ttttacaaaa tatttagcat cttttatttt accagcatta tataaatttt
    12781 ggataacaga accatttttt aaatgttgta aatggtcatc taacttataa gtatgtgcta
    12841 tatgagttac atttttaaat ttttcttcaa atgtaagagc aattttttcc atgtaattga
    12901 agtcagtatt accatttgta tttgtttctg gtaataatat tatattttcc cttattacac
    12961 caaggctatt aagagaagct aaaaattctc tatttctata ttctgatttt tgtatatttg
    13021 ttaggttttc atatttttta tatttatcaa agacggatat accacatcca gtagatacta
    13081 aaaccacaaa tacatgattt tttcctcttt cttttatagc attaacaata gcactcccag
    13141 cccacaacac ttcatcatct tgatgctgaa catagaagac aacataatct ttaaattttt
    13201 tatgactata attcacatag ctactaaaag cggttaaaat caaacataaa actagaataa
    13261 tagatagaat tttatattta tttatagtaa attttttatt cttaaatgtc atttaatcat
    13321 ctcctgaaaa gttgtcttag ttatattatc aaacaaagta ctaaaaatta tttcttaaat
    13381 gctgaaaaat atagagtaga ttaatggaat aaaatttttt tattagttta aaataaagaa
    13441 aacatttatg tataagacta atcaaaatat taatgattga taggaattga gatttaaagg
    13501 gattgtacat ttattcggga ttcattttaa tagaatttta ctttagtact tagaataaaa
    13561 gtaataactt tttaaattta aagagtacac atatatattt gtgtactcct tgatgatatg
    13621 attgcttatt tactacaaaa acagagtctt tttaaaccta aaatatttga tgcaccagct
    13681 atcgtagttc cataatagta aactggtttg ttaagtgata gaaaatttat aattgagtta
    13741 ttacataatg tacttcctgt gcaaatcact aaatcagccc aagatattac atcttcataa
    13801 tctcgtatac catcttcaat cttaacatta tattggatag tatccacaaa ttcaggattt
    13861 aaatctaata ctcttgtttc gaaaaagtcc ttaatattat caattatggc tggttgatat
    13921 cctataattg ctacttttgg attaccaaat tccattttta taaactcagg gaattttttt
    13981 gcacatatct ctggctcgtt atttttacaa tgaatggttc tatccgtctt gcctaagtat
    14041 ttcataactg catttaatga tgctataaaa agacttctat catgaggatt gtcatgtaaa
    14101 tctaatgata atatgtcttt taaagaacct tcaaatgtag atggagtaga agtaaatgct
    14161 tgtcctttag ctcctttaaa gtcagcttca agcattattt ctttaccaac tataattggg
    14221 aaatctttac gcttagtatt tccaattgct tcttttgaag atagagattt ggacacaata
    14281 cttatcatat ccatatttaa attatattta tcaacttgta ctaatagata atctctcaaa
    14341 atatcaaata tattcataga aaaaagattc tcctttattt tttatttctt catttaaatc
    14401 aaaaatatga tttatttttc cattgttcat tattcctatc ttatgagcaa aaaacattgc
    14461 ttcagaaata tcatgagtaa catgaatcac tgttggtttg tattttatgt agacatcctt
    14521 caaaattttg cttatatgtt tttttgtaat ataatccaat gaactcatag gttcatctaa
    14581 aagtaatata tcaggttcag ctattaaggc acgtgcaaaa gcaactcgtt gcttttcacc
    14641 tccacttaaa tttttaggat acctattttt tatatcttct atctgcatta atttagataa
    14701 gtggttagcc ttaagaatct gttcatctat aggtattttt ctcaatttaa gtggaaacct
    14761 tatattatct tcaacattca tatttggaaa taattcaaaa ttttgatata caaatcctat
    14821 atttctattt tctggaggta agtttgatat taaattttct ttgtatgata catttccttt
    14881 agcagatgga tatcttcctg ctaatgtttc tagaaataga gttttacctg acccactagc
    14941 tcctaataaa acaaagtatt catgattatt aatatttaaa ctattttctt gtaatttaaa
    15001 attaggtaaa tcaagagata aattttttat ttttaacata ttaattctta tcaacctctc
    15061 tattaaaaaa tatctcaaaa agcatcaagc atgtaagtga gataaatatc aaaattacag
    15121 ctattccgat tgcaatgtct atatcaccag tagacatatt taaatatata gatgtaggaa
    15181 taatttcagt tttcatacgt gtagaacctg ctatcatagc tactgcacca aattcaccaa
    15241 gtgctcttga ccaagtcatt acagttgcag atatgattcc agtttttaga tttggaagaa
    15301 ttataaatct aaatgcttcc caactattac atccaagggt acgtgcaaca aatatcatct
    15361 tttcattact ttcatttagt gaagtgtaaa gtattttaat agtaagtgga agatttacaa
    15421 atacttgtgc taaaactata ccttgctttg taaatataaa atcaagctta aaaatcttat
    15481 aaatggaatc tccaatcccc attcttccaa acaaaagtaa aagagcaatt cctgatacta
    15541 agtgtggaag agacataggg agatttatta tctgtataat cagtttttta aatggtaatt
    15601 tagtaatatg taaaaaatat gctactggaa tagctaataa aaggcatact atagtagata
    15661 ttaaagatgt tttaatacta agtattattg catactgtac ttccactgat tttaagcata
    15721 taggaacata acttatccct ttaatgatta acataagaag tggtgataca ataaacaaaa
    15781 agactatagc aaaaaatcca gtaagtagag tagtaaaaaa tttatttttc aagtttaatc
    15841 actacttaac aggttcaaaa ccatattttt taaatatctg tttagcttcg tctgactcta
    15901 tataatttat aaatttatta gctaaatctt ttttatcact atagtttacg attcctgctg
    15961 atataagttg gtctatattt ttatcttggg caatttctat aacttctaca tcttcattgc
    16021 caaagaccga atcttttgtg gctattgtag catcagcttt acttgatgtt aactgtacaa
    16081 tcatttcatt tacagttgca gctgtagaga caacattatc atttatagat tttaaattat
    16141 ttttttcaag tattttttgt gtagtcttac ctatagcatt agactctttg tctcccaaaa
    16201 taagtttaac tccatcttta tttaagtctt ccaatttttg aatattagct ggatttcctt
    16261 taggtacaac tattgcaggt gtatgatgtg caaccgtttt atgttcacct acaagttctt
    16321 tttcaaaggc ttttgaataa attggttcag aaccaacaat aaatacgtcc cctttgtgac
    16381 ttgtctcaat ttgagctatt agctgtgcag aacctgcata gctatattca actttgacat
    16441 tattttcttt ctcaaatttt tttgctattt catccattgg tttctttaaa cctgcccctg
    16501 aatagacaaa aagagtctct ttttcttttg aatcatcagc tgttgtcact ttagtattag
    16561 attcatcatt agctttgttt tctttagaat tacatgcagt aatacttaat gaaataatca
    16621 tagctaaagc tagtactgaa agttttttaa acataagata ttccccctta gtaaattaat
    16681 gtaaattctt ttttagtata tcatactaag aaaacgatgt ctaagttttt gttatgtttt
    16741 ataatgtttt gttatgtttt gttatgtata gttaagaaaa aatctattta taatgttatt
    16801 tctataggta gttattttat ttttaatttt tattaatact ttaaagtatt ttaaatttaa
    16861 aaacttaaat caaattaact tattgtagat tttttacttt ttaactaatt ttatatttat
    16921 tatatttcag tgaaaaaata gaacattgaa acatatagat atgttttaat tgctttttat
    16981 taacaaatta ctaaaaatgt tatatcagaa ttgaatacaa aagaaagagt atgtatttat
    17041 gagatagttg aaaattttga tgtgccacaa ctaaaattat tatattacta tatatttaat
    17101 taaaagagta cttgtaggtt taagtacata ctttataaat atatttttgt tagatttatt
    17161 ttataaaaag tatttagact aagtattatt tttaagtatt ttcttgttga agttattttt
    17221 tcctttttta caaaattcgt aaataaaaaa aagactctta aaagagtctt tccgaaaaat
    17281 ctatatatta taatatagtt atattttctg cttgaggacc tctagcgcct ttaactatat
    17341 caaagcttac tttttgacct tcttctaatg atttaaatcc tgaagtttgt atagctgaga
    17401 aatgagcaaa tacgtcatct ccactttcca ttgatataaa tccaaatcct ttttcattat
    17461 taaaccattt tactattcca ttattcatta aaaatactcc tattaaccta tatcaagata
    17521 tacttgattt acttgtgtat taccacaaat attattgtat catagattaa cataaatgtc
    17581 tatttaatta taatttttag ttaacatttt tatttattct taatatgaaa ataaatttaa
    17641 acattttata ttaccaatat attaatgtaa atgtagataa aactaaaaag ggttttctag
    17701 aaaaaaatct gtctaattat acttacttac caataccatc acttgatgca agcaagattc
    17761 cacaactttt tctccgtact ctaatataaa atcatctaac tgtgtatcta taaaatcaca
    17821 ataatcgtat gaattactaa tataagcagt taacatatgt gtacatttat ccattttatc
    17881 tctaaacata atgtatttat ccccctaaaa aactttcatg aaaaacaaaa agaaaatatc
    17941 tttttggcaa ctggctgaca taacttattt tataagcgtt agtccctata gctttgcgtc
    18001 actaaatttc tctagttttg ccgatttagt tttattctac agattaataa tacaatagaa
    18061 ttagttatca ttcaacatat ctagttgaat aattaataaa tttatatatg aactttaaat
    18121 tatttgaaaa aataaaaagc ccctctgtca ggagtgactt aaaaattgaa tttaagtttg
    18181 tatttaaaac acatttttaa taatgcaaaa actaatcaaa aataaacaat tcatcaatag
    18241 gtgtatctac tatttttgat atatctattg ctaatttcaa agaaggatta tactttcctg
    18301 cttctaaacg cataatggtt tctcttctaa ctcctacttt ttctgctaat tgttcttgag
    18361 ttaattcttt taattgtcta tattttttta ggttacaagt gaatttaggc atattatttt
    18421 tcctctaaaa tataaagttt tatagcataa acatttaaag tgattgcata tgtaattgat
    18481 agtaaaacac acattaattc tatattccta gacactatag taatacacca taaaatgaat
    18541 aattctataa tagcaagctg tcctgtaaat gccatagctg ctttttcatc ttgtacatac
    18601 ctttcatctg ctttatcacc atttattttt gctataataa agtttgagaa aaaagcaaaa
    18661 aatccaatat atgtaaggtc agcaacattg ccagatgaaa aatatctaag acttaaaaat
    18721 ccaaggaaac cacaaaaacc cataaaaaat tttttctgaa tcatttttta tcctccttag
    18781 taattttaaa agagatatat ttatatcttt atgatacaaa tatatctctt ttatattctt
    18841 ttgtcaatag aaaatcagta ttatacaata tttatactgc accataattg ttagagcaaa
    18901 aatctaatgc ttgagatgtt tatgtatgac taaatatgat tttaatttca aattaaaagt
    18961 agctaaattt tatttgaata gagaaggaag agatatttta atagtaaaac aatatggagt
    19021 ttatgaccat tctcaagttg aaagatgggt taatatatta ggagagagtg aactaaaaat
    19081 aaaaaaattt tctatactat atggttcaag ttagatgtta taaactatat gatgataact
    19141 aaatatagtg tacaataaat cgctaagtat tttggaataa acaatagcac agttatagca
    19201 acatgaaaat aaaaattttt acatagtggt gttgatgcct tttctaaaat gaaaggaaga
    19261 ccataatgaa taaaaacaaa aacactaaag aaaaaataaa ttaacaagag aacaagaatt
    19321 agaatatgaa aataaatttt taaaagatga gcttgagttt ttaaaaaaga tgtgtgtctc
    19381 agggaagcca cattaaaaaa cagtggtatc attattaatc aaaagaaagt tagatgtatt
    19441 atcaaaaaat tagaacttaa atgcatagca ttttttcata aatcacataa atataactcc
    19501 tataaaggta taattggaaa agtaaaaaat aataaaacaa atagaagatt taaaacatca
    19561 atagcatatc agaaaataac aaatgatatg ctagaattta taaaatatat ctaatgataa
    19621 aaatggaaaa tcaattataa aaaaggcata tctcgattca ttttttgtta tatagtcaaa
    19681 taagaatact atagatgaaa gatacgattt tgcatatgta ttaagtaagt attctgatgt
    19741 ttttggttga tacacatgca ataaagaagt tgtgtttgca gataaattag caaaactata
    19801 gtataagttg caacaataaa gaaaatgaaa attatcatac taaagcataa ggcatgttac
    19861 aattttatta gataggacat taacatacac taaagtactt tttattataa agagtactta
    19921 tttttttgaa attcattaat gtataaacta tcaagaatat tactcaacat aatttaaaaa
    19981 caacttgaat aggtattttt aaattatgtg atataataaa aaatagaagt gtatgccaat
    20041 acacttctgt agttataact aagcattcta ttcttgagga gtgctttttt catttttata
    20101 accaactctt tttactttaa caataattat cagacctata tatttatttt taattgtctg
    20161 atttattaga taaaaaagtt attatcatat ttttatcatt tggtattttt attgaactac
    20221 cataatcttc ttctaattta tctagaccac ttttacttat ttctccatct ataattatgt
    20281 ttaattctaa gtattcttca ctaccattag atgacactaa agtgtacgaa ccattataag
    20341 gcatgcctaa attttctatt ttatgtgcca aagggtcttc atcaccaata ccagagttta
    20401 ctgttatttt tccatctttt ttagtccacc atattatgtt tccttcagta tattttgatt
    20461 catctgcata gtagctaaga gacgcacttt ttattgcact tatatcagct tcaagtttag
    20521 ctatctttgc tttttctata tttttaaata atgctggaag tgctactatt gccaatattc
    20581 ctattatagc aattactact aataattcca ctagagtaaa tcctttttta tttttcattt
    20641 atttcccctc catataaaat taataatata taaattttta ttttgttaat ttctactttc
    20701 tatattttac catatataga caaaaaaatc actttctatg acaaatatac tattatttta
    20761 caatttatgg aaatgattta aaatgacatt tcttgttaga aaagatataa gaaatggatt
    20821 aaaataaaga aaactacagt catatcaatg tcaataatat tatttaaaag cagatgaaaa
    20881 taaaaagagg actcagaaaa taatgtctat ttaattatac ttacttacca ataccataac
    20941 ttgatataaa caagattata ctacattttt ttatgctcta gtataaaatc atctaactgt
    21001 gtatctataa aatcaaaata attatatgaa ctatcagtat aataagtcaa aatatgtata
    21061 catgaataca tattttaaaa gatatatatt attacatacc taaaaaactt tcataaaaac
    21121 aaaaataaaa tatgctttta gtaacttgtt aacataactt ataatttatt ttataagcat
    21181 tagcctatat agctttgtgc tactaaatct ctttgattct aacgatttag tgctattcta
    21241 caactaaaat aatacaatag agctatttat tgtttggtag cattgtttgt aaattaaaca
    21301 aatttataca tatgaatatt attttacatt caaaaaaaca gactaaatat gtagaatatt
    21361 tggtaaaact tacctctagt ttttgtctta aaaagctcct attattaact taagttagaa
    21421 gaattaagga agtgacataa attgaaagaa gaaatattaa tagaaaaatt attaagagca
    21481 gaattaatag taatggagta tttatggaaa aaagacactc taatgcctaa aaaagaaatt
    21541 gtaaaagaaa taaaacagat ttatggatgg cataaaagta caataaaaat actactaaaa
    21601 aggctagttg ctaaaaggta tttggctaga tatattataa attctcaatc tcattataaa
    21661 ataatagata gtaaagaata ttatactttt aagaaaaagg tactaaaatc tagtaaaagc
    21721 ataaaaataa tgcgttcact taccactaca cataaaaatg taagtaaaga gaagttagac
    21781 ctattagatg agtattatag aaacttaaaa gaatgatata gtaaaaatcg aatgtaatta
    21841 tttaccttaa ttcttttgtt tgtaaaactt tattgataca gaatttaaca tatacataaa
    21901 ttggtaaagg aatgtatact atgaaaatta gtaaattacc agaagcggaa ttaaaagtta
    21961 tgagatatat ttgggaatca gaaaaggtat taatatcaag agaaatcacc aagtctatgg
    22021 aacaaaaata tgaatggaaa gatacaacta tatttacagt attaaaaaga ttactaaaga
    22081 agagagtttt tagaagcgga gaaaatagat aagtgtacac actataatgt attagtaaaa
    22141 aaaaataata tcagagattt taaacaaaag aatttttaaa gaatacacat aaaaattcaa
    22201 tattaagttt aatgtcagca ctatgtagta aggatgatga agtagatggt gatattttaa
    22261 atttattaga agaat
//
LOCUS       DAYASV010000056        22252 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYASV010000056 DAYASV010000000
VERSION     DAYASV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.056"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            383..3037
                     /gene="adhE"
                     /locus_tag="ACGI68_002074"
                     /gene_synonym="adhC"
     CDS             383..3037
                     /gene="adhE"
                     /locus_tag="ACGI68_002074"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFH0138509.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            3313..5532
                     /locus_tag="ACGI68_002075"
     CDS             3313..5532
                     /locus_tag="ACGI68_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0138510.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNNGTIILHPQGDSLSKNIYKLLKQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFIAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            5786..6448
                     /locus_tag="ACGI68_002076"
     CDS             5786..6448
                     /locus_tag="ACGI68_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0138511.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            6819..8228
                     /locus_tag="ACGI68_002077"
     CDS             6819..8228
                     /locus_tag="ACGI68_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFH0138512.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLTSSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            8343..8618
                     /locus_tag="ACGI68_002078"
     CDS             8343..8618
                     /locus_tag="ACGI68_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HFH0138513.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            9248..9559
                     /locus_tag="ACGI68_002079"
     CDS             9248..9559
                     /locus_tag="ACGI68_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138514.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            9561..11486
                     /locus_tag="ACGI68_002080"
     CDS             9561..11486
                     /locus_tag="ACGI68_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFH0138515.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFNCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            11489..11980
                     /locus_tag="ACGI68_002081"
     CDS             11489..11980
                     /locus_tag="ACGI68_002081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFH0138516.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            12032..12595
                     /locus_tag="ACGI68_002082"
     CDS             12032..12595
                     /locus_tag="ACGI68_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFH0138517.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            12610..13587
                     /locus_tag="ACGI68_002083"
     CDS             12610..13587
                     /locus_tag="ACGI68_002083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HFH0138518.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            13580..13897
                     /locus_tag="ACGI68_002084"
     CDS             13580..13897
                     /locus_tag="ACGI68_002084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFH0138519.1"
                     /translation="MYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVEDEY
                     AIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGADIL
                     FKE"
     gene            14014..15792
                     /locus_tag="ACGI68_002085"
     CDS             14014..15792
                     /locus_tag="ACGI68_002085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFH0138520.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            15789..17162
                     /locus_tag="ACGI68_002086"
     CDS             15789..17162
                     /locus_tag="ACGI68_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFH0138521.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            17168..17836
                     /locus_tag="ACGI68_002087"
     CDS             17168..17836
                     /locus_tag="ACGI68_002087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFH0138522.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            18113..19162
                     /locus_tag="ACGI68_002088"
     CDS             18113..19162
                     /locus_tag="ACGI68_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0138523.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            19258..20247
                     /locus_tag="ACGI68_002089"
     CDS             19258..20247
                     /locus_tag="ACGI68_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138524.1"
                     /translation="MSYLKINNVFKSYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKGKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDASIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            20252..21970
                     /locus_tag="ACGI68_002090"
     CDS             20252..21970
                     /locus_tag="ACGI68_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HFH0138525.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAASLAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(22052..>22252)
                     /locus_tag="ACGI68_002091"
     CDS             complement(22052..>22252)
                     /locus_tag="ACGI68_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0138526.1"
                     /translation="HKEINNIINNINLKIVEENSTNELKLSMSIGYAIFDEKVDVSLF
                     DTYRRADSQMYDNKRKINKQYQ"
BASE COUNT         8593 a         2604 c         4079 g         6976 t
ORIGIN      
        1 gtacatttaa ctatatttac tttaacttgt taattaagtt ttaattttca ttttcaagga
       61 tttttatttt taaacttgat agagtatcat attaaaaatt ctttttataa agttttattt
      121 gcaaaaaata caaaaatata aacgttaaaa aaataacgtt atccttaagt ttttagatga
      181 tttcattagt gatttgcgta aaaaataaag tgatatctag ttaaattaag tatttgtaaa
      241 gctagatatt attagtagaa atgatgttat attagataaa aaataaaatg ttaaaaaaat
      301 aacttgaaaa taggaaaagt aaatgctata atgttaataa attaacataa taaataaaaa
      361 actatctcag gaggttgtta aaatggctca taaagaacaa gtaataatag aaaataaaaa
      421 agaaaatgaa gttatgtcag ttggtttaga tactgtaaat aacgttgaaa gcttagttaa
      481 aaaactagca aaaatcagag aagctcaaaa aatatttgct acatatagtc aagagcaggt
      541 agataaaatt tttttagcag catctttggc agctaataag caaagagttc ctctagctat
      601 aatggcacaa aaagaaactg gtatgggaat tgctgaagat aaggttataa agaatcatta
      661 tgcctcagag tatatatata atgcatataa agaaactaaa acttgtggtg ttatagagaa
      721 agacgaagca tttggtatga ctaaaattgc tgaaccaata ggtgttattg cagcagttgt
      781 acctacaact aatccaactt ctactgctat ctttaaagca cttttagctt taaaaactag
      841 aaatggaata attttctctc cacatccaag agcaaaaaat tctactatag aggctgcaaa
      901 agttgttctt gaggctgctg ttttagctgg agctcctgaa ggtataatag gatggataga
      961 tgagccatct ttagaattaa ctacaactgt tatgaaagaa gtggatttga cccttgctac
     1021 aggaggtcct ggaatggtta aatcagcata ttcatctgga aaaccagcta ttggagttgg
     1081 tgcaggtaat actccagcaa taattgatga tagtgcagat ataaaaactg ctgtaaactc
     1141 aattcttgta tctaagactt ttgacaatgg tatgatatgt gcttctgaac aatcggttat
     1201 agtattagaa aatatataca atgaagtcaa aaaagaattt aaagagcgtg gtgcttattt
     1261 attagataaa gatgaaacag aaaaaataag aaatataatt ttagtaaatg gaggattaaa
     1321 ttctaaaata gttgggcaaa cagcttgtac tatagcaaaa cttgcaggct ttgaagtccc
     1381 agtagatact aaggttttaa taggtgaagt agaatcagtt gaaatagaag aagcttttgc
     1441 tcatgagaaa ctttcaccag tacttgcaat gtacaaggca agtgattttg atgatgcagt
     1501 tagaaaagca gaaaaattag tagaagatgg tggatttggg catacatctt cattatatat
     1561 tgatgatgta aatcaaagag aaaaacttga taagtttact tcagccatga aaacttgtag
     1621 aattttaatt aatactcctt cttctcaagg tggtataggg gacttatata attttaaact
     1681 agctccttct cttactttag gatgtgggtc ttggggtgga aattcagtat ctgaaaatgt
     1741 aggagttaag catttactta atattaaaac agtagctgag aggagagaaa atatgctttg
     1801 gtttagagca ccagaaaaag tttatttcaa aaaaggttgt ttaggagtag cattaaaaga
     1861 acttaaagat gtaatgaata agaagagagc ttttattgta acagatacat tcctttacaa
     1921 taatggatat actaaagcag ttactgattt attagatgaa atgaatatta agtacactac
     1981 attctttgaa gtagaaccag acccaacttt ggaatgtgct aagataggcg ctaaagctat
     2041 gagagaattt aatccagatg taataatatc tataggtgga ggaagtgcaa tggatgcagg
     2101 aaagataatg tggacattgt atgagcatcc agatgtagat ttccaagacc tagctatgag
     2161 atttatggat ataagaaaaa gggtatatac attccctaaa atgggtgaaa aagctaattt
     2221 tgtagctata ccaacatcag caggtactgg ttcagaagta acaccatttg ctgttataac
     2281 tgaccaagat acaggagtaa aatatccatt agcagattat gaactgatgc caaatatggc
     2341 tatagttgat tctgatatga tgatgaatat gccaaaaagc ttaacatctg cttctggtat
     2401 agatgcttta actcatgcat tagaagcata tgtatctatg cttgcaaccg attatacaaa
     2461 tggattagct ttacaagcta ttaagagtat atttgaatat cttccacgtg catatgataa
     2521 tggtgcaaaa gacccagaag ctagagaaaa aatggcaaat gcttctacaa tggcaggtat
     2581 ggcttttgca aatgcatttt tgggtgtttg tcactctatg gctcataaat taggtgcttt
     2641 ccaccatgta ccacatggtg ttgcaaatgc tcttttgata acagaagtta tgaaatttaa
     2701 ttcttcagat gcaccaaaga aaatgggagc attctctcaa tataaatatc cagaagcatt
     2761 aaagagatat gctgaaatag cttcattttt aggattaaaa ggaaattctg atgaagagaa
     2821 attccaaagt ttattggtag ctattgaaga tttaaaactt aaagttggta ttcctaaatc
     2881 tataaaagag tttggcgttg aagaatctaa gtttatggat tcaatagatg aaatggtaat
     2941 tcaagcattt gatgaccaat gtacaggagc aaatccaaga tatccactta tgaatgaaat
     3001 aaaagatatg tatttaaatt catattatgg aagataagtt tgctataaaa tagataaaat
     3061 aaaaactctt atacataaaa caaagtttta ttgtataaga gtttttttgt ttgagaaaat
     3121 ggatagtttt tattctttaa tttaaaattc aattaaatag tgttattata ataataaaag
     3181 aatgttttta tggttattat gttgatttta tgtgtaacat ttaagaatag ttatatacaa
     3241 tataaatgat gaattggtat aaaaaggata tattatctat atgaagtatt gactaattag
     3301 gggaggagtc gaatgacagg gagaaataaa agttttacat taattacaat tcttttttta
     3361 acatcattat ttttgacaat ggggtatatc tatatagaag atacaaaaca tcttcttatg
     3421 tcagaggcaa aaatacatat aaaagaagtt gcagtacaag gctctcaact agcacaaaga
     3481 caaattgaaa aagatttaga tgttttaaat attttttcta actattatgc ttcaaatcca
     3541 gatataccta atgaggaaaa aatgaaaaat cttttagatg aaatggaaaa tcaaaaattt
     3601 tacactatgg caatagttga tataaatggt aatgctgaaa ataccaaagg tgataagttt
     3661 tcagtgaaag atagagaatt tttcaaaaac tcaattaaag gtaagaagta tgtttcaagt
     3721 ccatatgttg atgaagtaaa taagtctata aagaagattg ctatttcagt accattatta
     3781 aataatgata aagtagttgg agttttatat tgtacttaca atatcaatac tctgatgaaa
     3841 cttattaata tatcttttta tgaaaataat agtatatctt atgttgtaaa aaataatggt
     3901 acaattatcc ttcatccaca aggggatagc ctatcaaaaa atatttataa attgttaaaa
     3961 caggataatg atattcaaga agtaaataga ttaaaaaaag aattgcaaga aaataaaaca
     4021 ggtgcaactg tattaaatat gttggaagaa agaagatatt taggatatgc cactatggat
     4081 aatgggaata gtgaaaataa ttatataaaa gactggaatt taattttttc aattccagaa
     4141 actgttgtat ttagtaattc aaaacaaata atcaacagag ctgtatatgc agtgttatct
     4201 attgtattaa tatttattgc aattattttt tatataattt atataaaaaa atctaatgaa
     4261 aaagaaatat taagtttggc ttatgaagat aaagttacat atataggaaa tcaaaataaa
     4321 ttttatagag aatgtagtaa gtatctttta gataaaccat ctttaaatta tattatagta
     4381 tattttgata ttaataactt taagatgatt aatgatactt ttggatatga atttggtgac
     4441 aatttattaa ttacaatagc aaaagcttta aaagaagaat taacagaagg tgaggtatat
     4501 gcacgacttt caagtgatta ttttgcaatt ttctgtgatt ataaaaatgg aagaaataaa
     4561 attataagaa aacttgataa tatacgaagt aatattgaaa gtaatttgag tatagtcttt
     4621 gaaatttctc tgtgtgtggg tatttatttt gttgaagaag gtgaagtaga tattcaaaag
     4681 gctgtaaata aagcaaatat ggctaggtct gttgctaaag gtaaaaatat aaattatgct
     4741 atttataatg aagatgttag gaataagctt agtgaggaat caatgatttt agatgatata
     4801 aaaatagcat tggtaaaaaa tcaatttgaa gtgtattatc aacctaagtt ttcacttgta
     4861 actggagaaa tgatagggag tgaagcacta ataagatgga atcatccaga acatggtttt
     4921 ataagtcccg cagtatttat tccaatagca gagaaaagta agttgatttt aaaaattggg
     4981 agatttgtat ttgaaagggt ttgcaatgat ttatatgaat ggaaaaagca aggtaaaaag
     5041 atagttccag tttcagtaaa tttatctagg gtcgagttgt atcaaccaga tattgtaaaa
     5101 tttatcaata aaacaataaa aatgtataat ttaagttcag atttcattga aatagagata
     5161 acagaaactg tggctattaa tgagttaaat attttaaaga atgttttaaa tgagttgagg
     5221 acgtatggat tttctatttc tatggatgac tttggtacag gatattcttc tattagttgt
     5281 ttaagagata tgccaattga tatattgaag ctagataagt cttttcttgg tggcattgaa
     5341 catgatgaga gaagtcgcaa tatagcaaaa tcaatagtgt ctctagcaaa atcactagat
     5401 ttagttgtaa ttatagaagg tgttgagagt aaagaacaag ctgagttaat gaaacaattt
     5461 ggatgtgatt tagttcaagg attttatttt gcacgaccaa tgccagctaa gaatttttta
     5521 gatttacttt aaaacaaaat ttgattttta tataaagcac tattatgaat gcttgatttt
     5581 aataaatcat tgctaaataa ttagtgtttt tttattttat caatgtattg tctaatataa
     5641 agcaaaaaat aattgtattt tataaaaatt atattaaaaa taaatttatt ttgctaataa
     5701 acatgttatt aacaaaagaa tagtactata atgtatagat gataaaaact ttaataaaaa
     5761 tttagtgtat aggagttgtt taagtatgat agatataagt attgatgaca aacttaagga
     5821 aagatggcct aatgctaagc tgggttgcat tcaagcaaaa gtagcagtaa ataaaagctc
     5881 agaaaaatta attaatgaaa taaatgagtt ttgtgatact ataaatcaaa gtctaaaagt
     5941 tgaagacatt acgaaactag ataaaattaa agatgcaaga aatgcttata aagagcttgg
     6001 aaaaaatcca agtagatata gaacatcttc agaagcattg gttagaagaa ttattcaagg
     6061 gaagggtctt tatacaataa ataatatagt agaaataaac aatttaatat caataaaatc
     6121 attatatcca gtaggtacat ataatgtaag caaattacat tcaccagtat gttttactgt
     6181 aggagatgaa ggagaacagt ataagggtat tgggaaagaa ttaataaata tagaaaatct
     6241 tcctatatta tctgattcag tgggtaaatt tgggagtcca actagtgatt ctgaaagagc
     6301 aatgataaca gaagatgcaa atgaaatatt aatttgtata ttttctttta gtcaagaatc
     6361 tgatattgaa gaatattttg gatatgctaa ggaattactt aaagaatatg cagatggaaa
     6421 agatatagaa actaaaataa ttaaatagaa tctatataac aacgttatgt taagctaaaa
     6481 ttcaatataa aaataagtta agataaagaa actaacacaa aattagtttc ttttttaaaa
     6541 tagtagttta gtgttacaaa aatagataga tagtcccacg attattaaaa gtatggtaat
     6601 atattaatat agcagagatt gtgtttaagg gattttgaaa ggggggattt ataataatca
     6661 ttttatctaa aagataaaat ggttttatag aaataactat atatttaaaa atataaataa
     6721 tgatacagta atttgggtat ataaaataag gcatacatgg ttttgtttaa aaaccaataa
     6781 ggtacatagg taattacaat gtaaggaagg atttaattat gattgatgga aaagaggtaa
     6841 aacaagaaag tggacaaaaa aatctaaaat ttaagatagc tatagtagta ggtgttttgt
     6901 ttgtagctta tataggaatg gcaatctttt ttagaaatca tttttacttt ggaacgacta
     6961 ttaatggcgt aaaggcttca ggaaaaacag ttgagcaagt aaatgatttg ttaacatcta
     7021 gtactaattc ctatacttta aacttagagg aaagaaacaa taagaaagaa caaataaaag
     7081 caaaagatat tgatttaaaa tttacaccag atgataaagt tcaaaagata aaagatagtc
     7141 agagcgctat tggatggatt ggtggaatat ttaagtctaa agagtataat aatatgataa
     7201 ctctttcata taataaagat ttactagaaa aaaggtttaa tgctttatct tgttttgata
     7261 gtaaaaatgt tgtagaacct aagagtgctt atcctaagta tgatagcact aaaaacgctt
     7321 atgttatagt accagaggta cttggaaaca aagtgaaaaa agatgatttt tataagttag
     7381 tacaaaactc aattaattct gggaatactt ctatttctct ggatgaaagt aaagtttatg
     7441 aagcaccaaa caacacaaag gattcagaaa agttgaaaca agctattaag acattagata
     7501 agtatgttgg tgtagtcgtt acatatgatt ttagtgaccg taaggaaact ttagatggtt
     7561 ctgaaattaa taaatggtta aaagtagact cagaaaagaa ttatgacata acttttgata
     7621 atgatgctat ggtagaatat acaagaggtt taagtaaaaa atacagtact tttggtgact
     7681 caagaccatt taaaactgcc acaggtaaag atattacaat ttcaggtgga atttatggtt
     7741 ggttgataga taaaaagaaa gaagctgaag cattagttga tgttgtaaaa gctggtaaaa
     7801 atgttacaag agaacctatt tacatacaaa aagctgtatc tcgtaataaa gatgatattg
     7861 gaaatacata tgtagagatt agtctttctg gccaacatat gtggttcttt aaaaatggag
     7921 aaatgcttgt atcaacagac gttgtaacag gtgatttagc tagaggattt gctacaccat
     7981 caggtatata tcctcttaat tataaggcta gaaatgtttc attaactggt caaggttaca
     8041 gttcacctgt aagttattgg cttccgttta atgggaacat aggcattcat gatgctactt
     8101 ggagaaattc ttttggtgga agtatatata agtcttcagg ttcacatgga tgtgtaaata
     8161 cacctttttc taaggcaaag aaaatatatg aaaatataga accaggtaca ccaattattt
     8221 tatactagta aatgtgttgc taacagttaa gcgaagtcat cttagtttat ttaggatggc
     8281 ttccttttta tataaagctt attgaagttt acagaaagac tacttaaaag gagaaataca
     8341 taatgaatga aaaattaact aaaattttag aagaagctaa gaatgatgat gaatttagaa
     8401 aagaacttat aaaaacaaga gaacaacgtg atgttatgga ttcattttgt aaggttgtta
     8461 catctagagg ttatgatatg acagtagggg aacttttttc tatgggagaa gaatatacat
     8521 ccaatctact gaaaagtgta aatggaggag cagcatatcc tattgaagat tggagtgatt
     8581 ggtacgagga tttttttata atgttgtcga atatatagag taactaatga tattatctac
     8641 ttaagattaa tacattgcct taaaataaaa ctattagaac taggtgtaag ttttatcaat
     8701 ctttaaaaat attgatttat ttctaactag atataaaaaa attagattaa aaataaggtg
     8761 acaaaaatcg atatagcttg atgatttata aaagttagat aaaatagtgc gagatatttc
     8821 taaaaattca aaaaaaacaa taaacttcaa tggttagagg ttgaaaaaga atataaaaaa
     8881 cttttatatg taataaatta aattaaaatt ataaaaaaaa taaaataaaa atttaaaatg
     8941 tcgacataac gttaatttta aaacaaaata acgctattga caaactatat cgctttaaat
     9001 tataattatt gaaaataatg gtatttaaaa accatactca tgtcatagtc taaaagtaag
     9061 tcaaaaaagg atgtaaatct aggcaagtac tattctaaaa tatgttttat taagaaataa
     9121 acgttgaaaa atattaatta ttatataaaa atgctgttta tactttaaca atgaggtagt
     9181 aatctaatta gaataaaaaa atctaattgg ttatatacat agtaaaaagg taagagaggt
     9241 gatttggatg gcttttgaaa taataaaaag cattgttgag gcagagcaga cagcagacag
     9301 tatcaaagta aaagctgtta ctgatgcaga gtctatcaga gctgatgctg taaacaaatg
     9361 tgaaagcata tttgctgatg taaaaaaaca agcaaagctt atggaagaaa ctcttattga
     9421 gaaggcagtc accgacagta gagcagaggt tgataaaatc ttagctaatg ctaaaagtga
     9481 atgtctgaaa attgaaaaaa ctgctgaaga aagaaaaagt aaggctattg aagcggttat
     9541 tggaaaggta gtgagataat atggcaatag ttaatatgaa caaaatatct attgttggct
     9601 tagaagctca aaaaagccag attttaaagc tacttatgaa ccgtgggttt gttcagattg
     9661 atgatagtgc ctttcttact gaggaggagg aattaaaaaa ttatcttgta aaagatagtg
     9721 aagaatctga agttataatg ctagagcaga aaatgtctca tgtaaaccaa gcaattaata
     9781 ttgtttcttc actagtgaaa caaaaaaaat ccctacttgc accaaaaaga gagtttaaca
     9841 aaattgatag tgaaaaagaa gggcaagttt atgctgaagc tctggagtta aacaatttgc
     9901 acaaggagat aggtacaata aggtctaaca taaacttact taacaacaat aaaagtatgt
     9961 tagaaccatg ggtgaacttt gatattcctc ttgaaaatat ggaaactaag tatacaaaaa
    10021 ctatacttgg aactgtgccc ataagcgcaa acattgatgc tttaagagta aaattagaag
    10081 atgaaatacc agaaagtgtt attgggattg tcaattctga caagttaatg aactatgttt
    10141 atttaattac tttaaaagaa acttttgatg atgttttaga agttttaaaa gaatttaatt
    10201 tttcagtggt ttcattacca aatgaagaag gtacaccgtt acaagctatc aagaaataca
    10261 atgaaatgat aattagacag gaaaacgttt ctaaggacaa aacagaggaa attaaaagac
    10321 atgcagatag tatacacatg cttgaaaacc tatatgattt ttacactata gaaagagacc
    10381 aaaaaaagat tatagagaga cttgtcaaaa caaaaacaac attctgtctt aatggttggt
    10441 taccaagcaa aagggcagat gaattaatta aagaaattac agaaaagttt aattgttgtg
    10501 ttcagactga agaaggaagt aaagaagaag gcttcccaat tctgcttgaa aacaataaat
    10561 tagtaacacc ttttgaaagt gtaactaaca tgtacagcta tccaagtaca aaagatattg
    10621 acccaaatac aattttaact ttcttctttg ttgtattctt tggaatgatg ctaagtgacg
    10681 ccgcatatgg gttaataatt gcagtagtct gtggatttgt tgtatataaa ttaaagatac
    10741 aaaaaggtga aggaaattta attaaactta ttggtatctg tggagtttca actactgtat
    10801 ggggtcttat attcgggggt attttggggg atttaattcc tataaaagca ttaattaatc
    10861 ctttagagga tgttatgaca cttatgggaa tgtcgttact gttcggtatt atccatatat
    10921 atgtaggttt gggaataaaa gcatatacat taataagaag tggaaaggta atagatgcaa
    10981 tctttgacat aggtttttgg tatttgtgta taacaggtgt atgtctactt ataatacctt
    11041 ttgttgcagg ggatataggt gtattttcag aagtaggaaa atatttagca attataggtg
    11101 ctataggtct tgtacttact caaggaagaa gctttaaagg tattcctgtt aagcttttta
    11161 aaggattttc aagtctctat ggtataacaa gttattttgc tgatatatta tcttatacaa
    11221 gaattatggc gctttgttta acaacaggag ttatagcaca agttatcaac cttttaggag
    11281 ctatagctgg tccgatactt gcagttgtaa ttggtgttgt aggacacaca ataaaccttc
    11341 ttataaatgc actaggtgca tatgttcata caagtaggtt acagtatgta gaatttttca
    11401 ataagtttta tgaaggcgga ggagttcctt ttgttccgtt taagtataaa acaaaatata
    11461 caagcattaa taaaaaggag atgtaaatat ggaattttta aacgcgttag gaaatggtca
    11521 atttttggca atatcaggag ctgctatagc agcattattt gcaggtatgg gttctgctaa
    11581 aggtacttca atagcaggtc aagccgcagc aggtgttgta acagaagacc ctagtaaatt
    11641 cggtcaactt ctagttttac aacttcttcc tggtacacaa ggtctgtatg gatttatcat
    11701 agctttcctt attttatcaa aagcaggaat tattggtgga gatgcaatac caacatcagc
    11761 tcaaggttta caacttctta tggcaggact tccaattggt ttagttggtt tagtatcagg
    11821 tatagcacaa ggtaaagcag cagcagcagg agttggaata gttgcaaaaa gacctgaaga
    11881 acttggtaaa gctataacat ttgcagcaat agttgaaaca tatgctcttt taggactact
    11941 tgtttctttc ttagcttaca atggaattgc aattggttaa ttataaatta taattcataa
    12001 gtaaaaaaaa tgaaaaggag gaagcactcc tatgggtaat gaacaaaaaa tgattgacag
    12061 aattatagct gatgccaaac aagaagcaca agaaatattg gataaagcta aaagtgaagc
    12121 agatttaaaa gtaaattcag ctaatgaaaa agctgaaaag gaaatggctt cctatacaaa
    12181 gcttgcagag gcagaagcag aaaaagcggc ttctaaggaa atatcaggag cttatatgga
    12241 ggctaaaaag caaatattat caaaaaaaca agagatttta gaagaggtaa ttttagaagc
    12301 taaaaacaaa cttttaaatc ttaaagataa tgaatatgaa gagattatac taaacatgat
    12361 tgaaaaatca aattgtacag atgattcaga gatagtatta tctaaaaaag ataaaaaaac
    12421 attaaaagat gtgctatcaa aaaaaggcat taaagtgtct gatgaaacta gagatattac
    12481 tggaggtttc atagttaaaa aaggtgacat tgagtacaat tattcatttg aagccataat
    12541 tgcagtagag catgaatata ttgaacaaat tgcagcagaa attttgttta attaaggagg
    12601 ggttaaaaaa tggcagaaga aaaaacttat ccctatgccg ttgcacgaat aagagtgctt
    12661 gaaaaacagc ttcttaacag acaaatgttt attcaaatgg cagaagccaa aagccctgaa
    12721 gactctttaa gaattatagc tgaggcaggt tatgctgata catcaaataa taatatacat
    12781 aattttgaga atattttgac tcaagaatta tcaaagacat atgaaattct taaaagtcta
    12841 gctcctgaag aaaaatttgt tgatgtattt ctttataaaa atgactatca caatttaaaa
    12901 gtgctgatta aagaagaaat ttctggtgta gatggtgaga aatatttaat tgatggaggt
    12961 acaattcctc ttattaagct tagagaatct ttagcaaatc gtagttttag tgatttacct
    13021 aagataatgt catcagctat attagaggca tttgatgttt ataataagac acaaaatggc
    13081 cagatggttg atattgttct agataaagca acctttacca gtatgaaaga aactgctaaa
    13141 gaaagccaaa acaaatttgt aatagattat gttatgaaag tttgtgattt aacaaatcta
    13201 aagagtttta taagagtcaa aaatatgaag aaagattttg atatgtttat gaatgtgttt
    13261 gtttcaggtg gctcacttga taaggaaaaa ttcttagaag ctttttcctc agatactcca
    13321 gcatcatgtt ttaaatctac ttcttactct gatgtctgta aaaatggcat ggatagtggt
    13381 tttactgtct ttgaaaaact ttgtgatgac tatcttatgg aatatgttag aggagcaaaa
    13441 tttaagcctc ttacattaga accactaatt gcatatttat atgctaaaga atcagagata
    13501 aaaacaatta gaattatttt gactagtaag cttaataata tagacgcaga tactataaaa
    13561 gaaaggctaa gggatgctta tgtataaggt aggagtagtt ggagataaag atagcatcat
    13621 gggattttta gctcttggaa tagatatctt cccagcctat gatagtgacg aaattaaaaa
    13681 aagtatacat aagttggttg aagatgaata tgcgataatt tatattacag aacaggcatc
    13741 attgcttgca aaagaatcaa tagcaaaata caaagattac caacttcctg ccattatagt
    13801 tattccaggt attggaggaa gtatgggatt aggtatgaat gaagtaagag aatctagtaa
    13861 acgtgctata ggtgcagata tattatttaa agaatagatg aaagttgaat aaaagacaat
    13921 ctcaaatttt attggtgcta aatatatgtg ctaataaaat ataggaggga atatttaaat
    13981 tttgataaag tatatgattg caggtgagag aatatgaaaa caggcacaat tgtaaaggtg
    14041 tcaggaccgc ttgttgtagc ggagggcatg agagatgcga atatgtttga cgtcgtaaga
    14101 gtttcagata aacatcttat aggcgagatt atagaaatgc atggagataa agcttctata
    14161 caggtttatg aggaaacttc cggtctagga ccaggggaag aagtagtttc tcttggaatg
    14221 cctatgagtg ttgaacttgg accaggtctt atttcaacta tttatgatgg tattcaacgt
    14281 ccacttgaaa aaatgtatga tatttcaggt actaatataa ctaaaggtgt tgaagtatct
    14341 tcacttgata gaacaactaa gtgggaattt aaacctaaaa aatctgttgg tgataaggtt
    14401 gtagcaggag atataattgg tggcgttcaa gaaacagcag tagtagaatg taaaattatg
    14461 gttccttatg gagttaaggg tactataaaa gaaatatatt ctggtgaatt tactgttgaa
    14521 gatacagttt gtgttattac agatgaaaaa ggtaatgata tacctgtaac tatgatgcaa
    14581 aaatggccag ttagaaaaga aagaccatat agagaaaaga aaacaccaga ttcacttctt
    14641 gttacaggtc aaagagttat agatacattt ttcccaatca caaaaggtgg ggtagcagct
    14701 ataccgggac cttttggaag tggaaaaact gttacacagc atcagcttgc taaatgggca
    14761 gatgcagata tagttgtata tataggctgt ggcgagcgtg gaaatgaaat gacagatgtt
    14821 cttcttgaat ttcctgaatt aaaagaccca agaacaggcg agtcacttat gcaaagaact
    14881 gtgcttatag caaatacatc agatatgccg gttgctgcac gtgaagcttc tatatacact
    14941 ggtattacaa tagctgaata ttttagagat atgggatata gtgttgcact tatggcagac
    15001 tctacatcaa gatgggctga ggctcttaga gagatgagtg gtcgtttaga ggagatgcct
    15061 ggtgaagaag gttatcctgc atacttaggt tcacgtcttg ctcaattcta tgagagagca
    15121 ggaaaggtaa attgtctagg tatggatgaa agagaaggaa cacttacagc aattggtgca
    15181 gtttctcctc ctggtggtga tacatcagaa cctgtcagcc aagcaactct tcgtatagta
    15241 aaagtattct ggggtcttga tgcatcttta gcatataaac gtcatttccc agcaattaac
    15301 tggttgacta gttattcact ctatcaggaa aaagttgatg tatgggcaga taaaaatgta
    15361 aatgataatt tctcagctca aagagctcaa gctatgaaac ttttacaagt tgaatctgaa
    15421 cttcaagaaa tagttcaatt ggttggggtt gattcacttt cagatggaga tagattgata
    15481 ctagaggtta ctcgttctat aagagaagac ttccttcatc aaaactcttt ccatgaagtt
    15541 gatacttata catcacttca taaacaatat agaatgatgg gacttatatt aaaattctat
    15601 aagtcagcac aagatgctat caagcaaaat gttactataa acgatattat caagctttca
    15661 gttcttgaaa agattggtcg tgctaagtat gtagaagaag gcgatataga tgcagcttac
    15721 gattcaattg aagatgaaat agataacgag cttgcagacc ttattaaaaa gaggggggct
    15781 gaagaactat gataaaggaa tataagtcaa tacaagaggt agccggtcct cttatgttaa
    15841 taaacggcgt agaaggtgta aaatttgatg aacttggaga aattgaactt tcaaatggag
    15901 aaattcgccg ttgcaaagtt ctagaggtaa atggagatac tgcacttgtt cagcttttcg
    15961 aaagctcaac aggtataaac cttgctgaaa gtaaagttcg ttttttagga aagagtcttg
    16021 actttggagt ttcaccagat atattaggtc gtgtttttag tggaatgggt agacctattg
    16081 atggaggtcc agaaattata ccagataaaa gacttgatat aaatggagct ccaataaatc
    16141 cagcagcacg tgattatccg gccgagttta tacaaactgg agtctctgct atagatggac
    16201 ttaatacatt ggttagaggg caaaaacttc ctatattctc agggtctggt ttgccacatg
    16261 ccaatcttgc agttcagatt gcaagacagg caaaagtacg tggaactgat tctaagttcg
    16321 ccgttgtatt tgctgcagtt ggtataactt ttgaagatgc tgaatttttt ataagcgatt
    16381 ttaaagcaac tggtgcaatc gaccgttctg tagtgtttgt aaaccttgca aatgacccag
    16441 ctgtagagcg tgtatctaca cctagaatgg cacttacagc agcagaatac cttgctttcg
    16501 agcaagatat gcatgtactt gttataataa cggatattac aaactatgca gaggcgttgc
    16561 gtgaagtttc agcagctaaa aaagaagttc caggacgtcg tggatatcca ggatatcttt
    16621 atactgacct tgctactatg tatgaaagag caggaaaaat gaaagggcat gaagggtcta
    16681 taactatgat tccaatactt acaatgcctg aggatgataa gacacatcca attcctgacc
    16741 ttacaggata cattacagaa gggcaaatta tacttagccg tgaactttat aaaaaagata
    16801 tacagcctcc tatagatgtt ttaccatcac tttctcgtct aaaagataaa ggtattggta
    16861 aaggtaagac tcgtgaagac catgcagata caatgaacca gctttttgca gcttattcaa
    16921 gaggtaagga tgcaaaagaa cttatggcta tacttggtga atctgcactt tctgagattg
    16981 atttaatgta tgctaagttt gcagatgaat ttgagaaaga atatgtttca caaggattta
    17041 gaactgatag aactattgag cagacattag aaatcggatg gaagcttctt agtatgttac
    17101 caaaaagtga acttaaacgt atccgtgatg aatattttga aaaatatatg cctaaggagt
    17161 gatatctgtg gctagattaa atataaatcc aacacgtatg gaaatgacta gacttaagaa
    17221 actccttaaa actgctacaa gaggtcataa gcttcttaaa gataaacttg atgaacttat
    17281 gaaacaattt cttgagattg taagagaaaa taagcgattg cgtgaagaag cagaaaatgc
    17341 tcttgatact gcttataaaa actttataat cgcaagagca gttatgagtc aggagtatct
    17401 gggttcagca cttatgatgc caaagcaatc agtttcagta gatgtttcta cgagaaatat
    17461 aatgagtgtt gatgttcctg tgtttgactt taaaactgaa aacaatcaaa gtgatattta
    17521 tccttatggt cttgctttta catcaggtga attagattct gctatggaag ctttctcaga
    17581 tgctatgcaa ccacttttaa gacttgcaga aagtgaaaaa agtgcacagc ttcttgcaca
    17641 agagatagaa aagacacgta gacgtgttaa tgctcttgaa aatgtaatga taccaaatta
    17701 cattgagact attaagtata tagctatgaa gcttgaagaa aatgagagag caagtactac
    17761 tagacttatg aaggttaagg atatggtgct taaaaaagca cttgaagaaa agaaaaaaaa
    17821 tgatttggtt gtataattaa atacttataa aaataagtct aacattctac tttatagagg
    17881 ttagacttat tttttatgtt taaattaaaa tgtagatact ataaaaatta atatattgtt
    17941 taaaaacaga ctgtatttaa ctaaaaataa tatatttaac aaaaatttct aaaaattaac
    18001 gaactgttaa tttacactta actttatatt aacaaattag gtgtattata taaacataag
    18061 caaaaacaat aaaaaacaaa taaaaaaagg aaaaaacaca aagggggaag atatgaaaga
    18121 atttatattt acttgtctgt gcattgtagt gattacattt atgacaagtt gcatatcaac
    18181 aacaccacaa actactcaag gaaaaggaaa taataaactt acaatatatg cagcactaga
    18241 ggaagagcag atagcttatt atatggaagg ttttagagaa gagtatccca atatagatgt
    18301 agagatagtt atggattctc atggtgtaat agcttcaaaa ctattagcag aaaaagataa
    18361 tccacaagca gatattatat ggggattatc agcaatgaac atgatagagc taaaaaaaga
    18421 tggtgcactt gaagcatata agcctaaaaa ttatgataaa gttgataaaa aatttaaaga
    18481 tactgaagat gatgtatctt gggtaggaat gagtgtccca gagacagcaa tagtagttaa
    18541 ttataaggaa cttaagaaac taggtataga tattccaaaa agttatgaag atttaataaa
    18601 accagaatat aaaggactta taacaatgcc aaatccagca tcatctggaa cggggtatct
    18661 tacagtatca gggcttattc aaatgatggg tgaaaaagat gcttacaatt acatggataa
    18721 attacataaa aatatagcca catatacaca ctcgggttca aaacctgcga agttagctgg
    18781 ctctggagag tatccaatag gtattacact tggatataga gctacacaac aattatctag
    18841 tggagaacct atagaggttg tatttccaaa ggaaggttca ggctgggact tagaagcaaa
    18901 tgccttagtt aaaaaagata atataaaaaa agaagcaaag ctatttttag attgggcaat
    18961 aagtgattca gctatggata gatatgcaca tgaagtagca gttacttcca taaagacaga
    19021 taatccagtt ccaaaaggtt attctaaaca accatttgaa cagcttataa aagaagttga
    19081 tttaaactct gctgccaaaa acagaagtac agtacttaaa acttgggaat caaaatacgg
    19141 ttcaaaatct gaaagtaagt aaagtttaaa ttaataattt agatgaaaga attttagaag
    19201 aaaaagtagg atataaaatt taaaataaaa aaataactat ataaggagag ataagatatg
    19261 agttacttaa aaataaataa tgtgtttaaa agttatgacc aaaaaagggt tttaaataat
    19321 attagtttgg atattgaaga aggagaattt ctatgtttac tcggacctag tggatgtggt
    19381 aaaactacat tacttagaat aatagcaggt cttgaagatg taaatagcgg aactataatt
    19441 ctccaggata aagatattac aaatttagag ccatcaaaga gaggttttgg tatagtattt
    19501 caatcatatg cattatttcc gaatatgact gcttataaca atatagcttt tccactaaaa
    19561 gagagaaaag tttcaaaagg aaaaatagat aataaagtta aggaagtact tgaaacagtt
    19621 ggattgacta atgaagctca taaatatcct aaagcattat caggtgggca acaacagagg
    19681 attgcaatag caagagcatt agctcttgaa ccaaagtttt tacttttaga tgaaccaatg
    19741 tccgcattag atgcaaaggt tagacacaag cttagaatgg acattaaaag attacaaaaa
    19801 gaacttaata taacgacaat aatggtaaca catgaccaag aagaagctat aactatggca
    19861 gataaaatag ctattctaaa tggaggagat ataatgcaaa tagggactcc agaagaaata
    19921 taccaaaatc ctcaaaacct atttacagct caatttatag gagatacaaa ctgttttgat
    19981 aatggagatt ctatattaac tgtaagacca gaatatgttc aaatagagaa atcgacaaaa
    20041 gaaaactacc aaggaataat atcaaatata gagtttagag ggaatttatt gagagttgaa
    20101 ataaaagata aattaaatga aaatttcata ataagtgatg cttctataaa agagtgggtt
    20161 aatttaaatt tagtagaagg tgaccttgta aaaataagta tagacgaaaa atactattta
    20221 aagtatccaa aagttaaagg agcataagtg tatgaataat aatgctataa gttctaaaag
    20281 tataagtctt aaatcaaaaa tgaatttaga tataaaaaaa tgtatgctta cagtattagt
    20341 tatgtcttta gtaatatttt tattagcacc tataatatat ctatttgtac aggctttttt
    20401 agataaagat ggagtctttg taggattaca aaactttcaa gaatacttgg cttctgaatc
    20461 tctattaaat tctctaaaaa atacagtatt tgtatcagtt atatcaactg taatatcact
    20521 aacacttgca ttagtatatg catatgcatt aactagaaca aagattaaat ttaaaggttt
    20581 ttttaaatca ttagctatgt ttcctatgtt tgctccaaca atgatgcatg caataggtct
    20641 agtatattta tttggaaatc aaggtttggt aactaattta cttggagtgc atgttccaat
    20701 atatggagca gtgggaataa tattggcaca aattatatat gtatttcctc aatcatttca
    20761 aatactttat atatctctaa gttcaaccga ttatagacta tatgaagcta gtaatactat
    20821 ggggataagc aagataagag aatttataag cataacaata ccaagtataa aatactcttt
    20881 aataagttct gcattcgtat cgtttacatt atgttttaca gattttggag ctccaaaggt
    20941 gataggagga gagtttaatg tattatcaat agatgtatat aagcaaatta tagggcaaca
    21001 gaatatgcca atgggagcaa ctgtatcaat attactttta atacctgcaa taatatcgtt
    21061 tatagtaact aaaaaaattg aaaaaaatca aaattcatat ataacttcaa agtcaattcc
    21121 atataaggtt aaaactaata agttaagaga tagtatttta ggaggggttg taggaattat
    21181 tgcatttggt ataatagctg taatgttaac tgtaatctta tcttcattaa tatcagtttg
    21241 gccatatgat ttaagactga cactggaaca tttttctatg aaaaacagta ttgatgggat
    21301 taaaccattt ataaattcaa taaaaatgtc actattgaca gctattctag ggacgagctt
    21361 tgttttctta tttgcatatt tgaatgaaaa aacagaaaaa gctccatatt taaaatcatt
    21421 tgggtatttt ttaagtacat tgcctatggc attgccagga ttagttttag gtatagctta
    21481 tgtattattt tttaataaat tagagtttaa ttttatggat acaatataca ttaagaattt
    21541 gtttaatgga ttatatggaa cattacttat aatggttata tgtaatatag ttcatttctt
    21601 ttcagtagca tttataacag ctactactgc tattaagaaa gtagataaag aattggatat
    21661 ggtagctaaa tcaatgggaa taaacagcgc atcaattctt aagaacgtaa ctataccact
    21721 ttgtttgcca gcagttttag agaactttat gtactttttc ctaaactcca tggtgacaat
    21781 atcagcatta gtttttatat atactgcaag ttcaaaagta gctgcaatat caattgtaca
    21841 actagatgat aaggggagta ctgcacaggc agcatcacta gctgtgttaa tattagtcac
    21901 aaatatatta gtgaaagtaa tatatgaatt tataaagaat cgtttagaaa aaagaacaaa
    21961 ctttaattaa ataatttatc ttataaaaac acaaatttaa aaaagtctat tcttgtgatt
    22021 tattatttaa agaatagact ttttaaattt attattgata ttgtttatta attttccttt
    22081 tgttatcata catttgagaa tctgcacgac gatatgtatc aaataaagaa acatctactt
    22141 tttcatcaaa aatagcatat ccaatagaca ttgataattt taattcatta gttgagtttt
    22201 cttcaactat ttttaaatta atattattta taatattatt tatttctttg tg
//
LOCUS       DAYASV010000057        22247 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYASV010000057 DAYASV010000000
VERSION     DAYASV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.057"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(134..1324)
                     /locus_tag="ACGI68_002092"
     CDS             complement(134..1324)
                     /locus_tag="ACGI68_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0138527.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1584..2015)
                     /gene="dut"
                     /locus_tag="ACGI68_002093"
     CDS             complement(1584..2015)
                     /gene="dut"
                     /locus_tag="ACGI68_002093"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFH0138528.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2128..4356
                     /locus_tag="ACGI68_002094"
     CDS             2128..4356
                     /locus_tag="ACGI68_002094"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFH0138529.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4467..4952)
                     /locus_tag="ACGI68_002095"
     CDS             complement(4467..4952)
                     /locus_tag="ACGI68_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0138530.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5204..6427
                     /locus_tag="ACGI68_002096"
     CDS             5204..6427
                     /locus_tag="ACGI68_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0138531.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPSILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6431..9565
                     /locus_tag="ACGI68_002097"
     CDS             6431..9565
                     /locus_tag="ACGI68_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFH0138532.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGKKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9587..10897
                     /locus_tag="ACGI68_002098"
     CDS             9587..10897
                     /locus_tag="ACGI68_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFH0138533.1"
                     /translation="MNKKMSKIVAIGCGVGIITANLIPVYADNNTSSINKVDNSILSD
                     SNKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLTDKYEKDYKRYEKEVEAYNLERQTYAAGLTTYANYLQQKFN
                     TENGLVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11186..11431)
                     /locus_tag="ACGI68_002099"
     CDS             complement(11186..11431)
                     /locus_tag="ACGI68_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0138534.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11645..14272)
                     /gene="ppdK"
                     /locus_tag="ACGI68_002100"
     CDS             complement(11645..14272)
                     /gene="ppdK"
                     /locus_tag="ACGI68_002100"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HFH0138535.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14333..15175)
                     /locus_tag="ACGI68_002101"
     CDS             complement(14333..15175)
                     /locus_tag="ACGI68_002101"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFH0138536.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15182..15820)
                     /locus_tag="ACGI68_002102"
     CDS             complement(15182..15820)
                     /locus_tag="ACGI68_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138537.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16256..17548)
                     /locus_tag="ACGI68_002103"
     CDS             complement(16256..17548)
                     /locus_tag="ACGI68_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFH0138538.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDIDLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17585..17941)
                     /locus_tag="ACGI68_002104"
     CDS             complement(17585..17941)
                     /locus_tag="ACGI68_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFH0138539.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17976..18335)
                     /locus_tag="ACGI68_002105"
     CDS             complement(17976..18335)
                     /locus_tag="ACGI68_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFH0138540.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAVISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18404..20377)
                     /locus_tag="ACGI68_002106"
     CDS             complement(18404..20377)
                     /locus_tag="ACGI68_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138541.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDEKFRKNIVNREEKSPTVFD
                     KGLLFPHSINEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(20599..21612)
                     /locus_tag="ACGI68_002107"
     CDS             complement(20599..21612)
                     /locus_tag="ACGI68_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFH0138542.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(21625..22170)
                     /locus_tag="ACGI68_002108"
     CDS             complement(21625..22170)
                     /locus_tag="ACGI68_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFH0138543.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         7624 a         3724 c         2881 g         8018 t
ORIGIN      
        1 aaaataaata aaaagcaggt atcaatcaaa tagtaacatg tattatacta tacattacta
       61 tcaatattgt acctgctttc atattataag caataaagaa aaatctattt attaggtttt
      121 ataataaaaa caattataaa actcttcttg catttacata tctacttgaa tagtagctac
      181 tatttatatt agatattgtt actttttttg atgaacctga agatgcatgt atcatatctc
      241 catttcctat gtatattcca acatgactta cagaaccatt atttgaacct tgtgtatcaa
      301 agaatactaa atctccactt ctaaggtctc ctctatttac atatgttcca tacttacttt
      361 ggtctctaga tactcttggt atactaactc ctacagattt tttcataacg tactgtgtga
      421 atccagaaca gtcaaaacta tttggacctt ctgctcccca tacatatggt tttccaagta
      481 gtgtctttgc aaaacttatt acactatcag ctccttgact tggagaatta gtcgaataag
      541 atggaattga agtatcaaca gaaccttttt cagcaaggta ttgacttgac acatatccag
      601 tttgattgcc ataacttact tttgtccatc ctccactttc agaaatactt ccaacctcag
      661 taccataact taatgtagct attttagaat gacttgtacc tggtcctgtt ctaacgttca
      721 atgactttgc tgttacaacc tttgtaccct ttacaaggtc atttgaactg ttttcagcat
      781 tttcatttgt agagtcattt acactagttc ctaaatattt acttgaaaca taacctactc
      841 taccattaga acttattttt gaccatccat ttgactcaga tatgtatccg acttcagtcc
      901 catatcctag tgttgatatt ttagaactac ttgtacttgg tcctgttcta aaatttaatc
      961 cttttgctgt tacaactttt gtcgacttaa ctgaagtatc actactttca ttgctacttc
     1021 caaggctatt tattgctaca taatttccat gcacataacc tgtgtttcca ttatatttaa
     1081 cttttaccca agaaccttct tttcctacgt actcaacttt atcacctttg taaagtttcc
     1141 ccattgattc atggtttgta cctggtcctt ttctaaaatt taccgcattc cctgttattg
     1201 ttctgctttc tatactcaca ctctctactt cagactcatt agcatatgct aaattcccca
     1261 tagtaggcat tattatcgca gttgccatta tactggctgc tattctcttt tgattaaaat
     1321 tcataacttt ccactcatct cctcttttct actatcttta tttcacttca aaatataaaa
     1381 gaaataattg acactattaa aatatcaatt atttctttta tattaatatt attacaaatt
     1441 tgtaactatt tgtctatagt aatatttgct taccataaat taccatgtat aaattttaat
     1501 ctttaatagc ctgtaattta gtaatatagc catcttttac cttttttaaa aatggctata
     1561 ttttttaact tttttgtaac tttttaaaat ccagttgagc caaacccacc ttttcctctt
     1621 tcggagtcac ttaattcttt tacttcttct atgttaatat catagatagg ttttactatc
     1681 atttgagcaa ttttcatatg cttttcaacc ttaaaatcat ttttaccatg atttattaat
     1741 ataattttaa gctctcctct gtatccttcg tcaatagttc ctggtgtatt taatacagta
     1801 acagaatgct ttaaggcaag cccacttcta ggtcttacct gcgcttctgt catagtagga
     1861 agctctatac atatcccagt tttgataagt ttagtctctc caggaggtat tacaacctct
     1921 tctatagaat acaaatccat accagcatca cctttgtgag caaaattagg tattatagca
     1981 tcatcattta atttttttac ttttaaattg tacatcttat atctcctttc aactttctat
     2041 aaatattata acttaaattt cgacctcttg aagtttttta tttttttgtg gtaatataat
     2101 tattagtgtt ttaaaggagg attttttatg aaacttgtac ttgctgagaa accatctgtg
     2161 gcaaaaacta tagcatcatt tttaggtgca aagaccagac aagatggtta ctttgaagga
     2221 aatgattata taattactta tgcagttgga cacttagtat cattatatga tatgaaggat
     2281 tatgataaag ataaatactc tggctcttgg aaaatgaaca atttcccatt tgttcctgag
     2341 gataagttta aatttaagat tgatagttct aaaactaaac aattcaacac tgtaaaaaag
     2401 cttttgaata gaaaagatgt agaatatgta ataaatgcca ctgacaatga ccgagaagga
     2461 gaacttatag catttttaat atttttactg gcaaaaaata aaaaaccagt aaaaagaatc
     2521 ttggtaaatg aatggacacc agaagatatt acaagaggta taaaaaacct aaaagatgaa
     2581 gatgaaatga gaaatcttca agctgctgga tatactaggc ttattactga ctggttgatt
     2641 ggaataaatt tcacttctgt tgctacattg aaatatggaa atgggaaact attaaacata
     2701 ggtagggtaa ttcttccaac agttaaatta gtctatgata gagatatgga aatactaaac
     2761 tttgttccta aaacctatta tgaaatagaa gggcatttta aagctgaagc tggtgaatat
     2821 aaaggtaaat atgttaaggg taaagaaagt aaatttgata cacttgaaga tgcaaataaa
     2881 attatagctt caattacatc tgaaactggt aaaatactag ataaaaaagt tactatgtca
     2941 aaggaatatg caccaaaact ttttagtttg acatcacttc aaggatatat aacttctaag
     3001 tattccaatt ttacatctga taaggtttta aatgtctgtc aatctctcta tgagggtaat
     3061 ggtaagggtg gatacataac ttaccctaga actgattcca ttttcttaga agaaagtttg
     3121 gcttcaaaag cttctcaaac tttagataaa ttaaaggttg gtcttgaata tgaaaataaa
     3181 attaaatttt ctaaaactaa aagagttttt gattcttcta aagtagactc ccatagtgct
     3241 ataattccaa cttatataat tccaaaaagt ttatctaagg atgaacaatt agtttatgat
     3301 gccataaaag atagatttgt agcaaacttt atgcctcctg ctgaatatga gaatacagaa
     3361 attaagactg aagtagataa ttgtactttc ttaacaaaag gtaaggtgct taaatcaaaa
     3421 ggataccttg aggtatataa taaggaagaa aaaaacgatt tacttccact tgtaaataaa
     3481 gatgatgttg tagatgtgtt agaaataaaa ccattaacta agcaaactac tccacctaaa
     3541 ccatatactg aggacacact tttaaaggct atgaaaaatt gtggtaaaaa tgtgcctgaa
     3601 gaagatacaa cagtgctatc tggatattcg attggaacat cagccacacg tgctgatgtg
     3661 ttaaagaaga taagtcaagt gggctatgta aagaaaaaag gtaaatctta ttctataaca
     3721 gaccttggga aaaatttagt tgaaattttt cctgttaaag acttatttga tgtagattat
     3781 actggaaaac ttgaaaaaag tttaagcgat attcaaaaag gaaagtatac aagaaaagaa
     3841 tatcttacta atatcattag ttttatatat caaaatgtca atattataaa acaagatgct
     3901 tcaaaaaata taaacacaga agcatatact ttcgattcaa aaactaaaaa atttactaaa
     3961 acaaaagaaa aaaaacagaa aaaagatgta actaatactg aaaaagaagt tgctatatca
     4021 aaggattcta ataacaaaaa agaagttaaa aatggtgatg aaaatcaatc tcttggcaaa
     4081 tgccccattt gtcaaggtga tgtactagaa tttgacaaag gctttgcttg taaaaatcat
     4141 aaagaatgta aatttgttat ttggaaaaat gataagtatt taagtcttta taaaaagaaa
     4201 gttaacaaaa caatggtcaa aaatatttta aaaaaaggtg aaactaaagt aaaatcttta
     4261 actgctaaaa acggtaataa atttgatgct acattaaaat atgataaaaa tccagatact
     4321 ggctatttca attggaaaat agagtttgac aactaaatat tcataagaaa agaaatagca
     4381 gtagtataat aatctatatt atattactgc tatttctttt cttatgaagt tttaactttt
     4441 ttaatagtga cttttacaat cacactctaa cctaatttct ttactatttc tgataacttt
     4501 aatgtatgtt catctaataa agttagttcc tcttcagaaa gattagataa tttttttact
     4561 agagattctt tgagaatagc ttttactgca tcaaataatt taaatgcttt atctgttaat
     4621 tcaaccctaa gaatccttct atcttttggg tcaggaaatt tattgactaa accatgttcc
     4681 actaatctgt caataatcgg agtcatattt gattttaaaa ttcctaaatt agaagctatt
     4741 tgagaaattg atgatgaatt tgatgtcata agataaagaa ttaccttagc atgtgatgga
     4801 gccattggac attcttcaaa ataagctttc attatatcag agttattttg aaactttttt
     4861 aacaaatcgc tttctttaaa tatattattt tcaagcacaa ataaaaaatc taaaaaagag
     4921 tccgctattt taagtatatt gttgttatcc atatttgtaa catcccttca atagataata
     4981 taataaaaca ttgttgttta atatagtata aattataaac tattaataaa tttaaaacaa
     5041 gaaaaagtta ttgactcata ataattataa atttataatc aaattagaga attataaatt
     5101 gccaataaag ggaagttttt taattagtct aaaactaaac ttaaattata agacacttta
     5161 aattctacaa caagctagtg tgcaatatat aataggagga gatatgaaaa gatatttaat
     5221 agttttctta atgtgtagtt cactgttttt agttggatgt ggaaaaacag aagaatctga
     5281 acctgagaaa ccaatagcag tttctgttca aaaagcaata ggtggagaaa ttgaaaacac
     5341 aaattctttc tcaggaacta caaaggttaa agatgaaaca gctgttactg cacagactgt
     5401 tggcacagtt caagaagtgt atgtaaaact tgggcaaaat gtaagaaaag gagatgaatt
     5461 attgagtata agcagtccag agctagaaaa tagtgtaaag caaagtaaag cctcactaga
     5521 tttagcaaaa gcaagttatt caagtgccac tggtggttca ttagaggcac aagtaaatca
     5581 ggcaaaaaca gctcttgaca atgcaaagat tcaatatgag gagtctcaaa gaaattatga
     5641 caataataag attctttatg aacaggaagt cattagttta gaccaattta aaaaaataga
     5701 attttcttta gagcaaacaa aacaacagtt agattctgct caaagagcat acgatacagc
     5761 aacttcaaaa agtatacctc aagctaaagc acttgctaaa aaacagcttg accaagctca
     5821 agtatcatat aatttagcaa tgagtaattt agacaaatta acattaacat ctccagtaga
     5881 tggtacaata acagctaaaa actttgattc aaaggaaatg ataacacaat cacaaccagc
     5941 ttttattatt tctaatccta gtatactaga agttgattta aatgtagcag aatcagatat
     6001 aggtaaattt aagaaagatg gaaatgtaga tgttataatt gaagaccaaa ggattttagg
     6061 aaaaatagat tatgtacctt cagtagttga ccctcagaca tctttatatc ctgttaaagt
     6121 acttgtaaat aatacaaaca acaaatttaa ggcaggtatg tctgctcaag taaacttaag
     6181 tgttgaaaaa gaaaatggtg ctgttacagt acctaaaaaa gcaatatttg aagaaaatgg
     6241 taaaaaatat gtatacatag caacaaaaga caatatagca aagaaacact tggttcaaac
     6301 tggaattgta acagaggata aaatagaaat taaaagtggt gtaagtgata aagatacagt
     6361 gattattgga gggataagtc ttatttctga cggaactaag atatttcctg tagaaaagga
     6421 gaagtagact atgaatttaa cacagacttc cgttaaacgt cctctcacaa tcataatggt
     6481 ctttttagta ttaatagttt ttggtggtat tggatataaa aaaatgtcaa taaatctaat
     6541 gcctgatata gaaataccag ttgtaatggt catgactact tggacaggtg ctggtcctca
     6601 agacgttgat gaacaagtta gtcaaaaagt tgatgaaagt ttatctgccg tttcaaatgt
     6661 aaaatctact atatcaagtt cacaagaaag tgtttctatg gtagttgctc agtttgaatt
     6721 tggaactaat ttggatgaaa taatgaatga tgttaggtct aaagttgatg cattacagac
     6781 cagcctccca gatgatgcag ctaaaccaac agttttaaag ctggatatga atgcacaggc
     6841 cataggtcaa cttgtcatat ctggaggaaa tgaaaatagt tcacaagcct taagaaaata
     6901 cgctgaagat gtaattcagc ctaaaataga atccatagat ggtgttactt cagcagacct
     6961 taaaggtgga aaaaaagcac aggttaatgt aatagctgac ccagctgttc tttcaaatta
     7021 tggggttagt ttatccacta taaaaggtgt cttatcctct agtaataaaa cattccctta
     7081 tggttcaatt actcaaggtg aggataaaat agttttaaga gcgattgata aattagaatc
     7141 cctagatgat ataaaacaga ttcaaattcc tgtaaaagga ggaaatacag ttagattaga
     7201 taatctttgt agtgtagatt atggatatgc agacaaagat agtatttata gatacaatgg
     7261 aaaagacagt ttggtaatag atatttctaa acaacaagat gccaacactg taaaagttat
     7321 gcagagtgca tacaaatacg tagatgaatt gaataaggaa aatcctcaat ttaaaattaa
     7381 aattgctaat gatacaagtt catacataag tgaatcaata aacagtgtaa tgagcaactt
     7441 gcttataagt gctgttatcg catttattgt aatatttgct ttcctaaaaa gtgtaagagc
     7501 ttcactagtt gttgcagttg caataccaat ttctataata ggtgctatag caattctcta
     7561 ctttactggt gaaagtttaa atcttattac tgcaagttca ttggtaattt cagttggtat
     7621 ggttgttgat aatgctacag ttgtaattga aaatatattt aaatatagga aaagtggcag
     7681 attgagtctt gatgattgtg ctattgaggg aacaagaact gtaacaaatg caatacttgc
     7741 atcaacattg accacaatag caatattctt acctatcatg tttacagaag gtatagctaa
     7801 aataactttt ggggcattag gtaaaacact aatagcttca ctagcattct cctttatagg
     7861 tgcaattaca ctcgttccta gtgtatttgc aaaattaagc agaggaaaaa atagtcaaaa
     7921 gatgcaagaa aaagatagtc ctatatttga taaggtaagt gaagcatata aaaaattatt
     7981 aagtgtatgt ttgagacatc aacgcttagt tgtaatatca agtttactta tttttgtagc
     8041 ttccttattt ggaatgggat ttataggtat ggactttatg cctgcagcag acaagggtga
     8101 attatctatt tctatagaac ttccaaaagg tcttagccta gaatccaatg attactatgt
     8161 atctatgact gagaaaaaag tctcagatat tccagaaatt aaaactttaa taacaacttt
     8221 gacatcagat tcaaataata gtaaagcaag tatagcaatt gaattagaat ctcaaaaaaa
     8281 tagaaaaaga actactgatg aaatagaaaa agagataatt gatagagtgg ctactgtacc
     8341 agattgtaaa ataaatgtaa gtcaaaacag tagtgttatg ggtggagctg gaggagcaga
     8401 cttcacatta gaaatgaaag gtcctgattt agatgtatta aaaatactgg gaaaagaagc
     8461 tgaaaaagaa tttagctcta taaaaggatt tagaaatgtt gaaacttctc ttacagatac
     8521 aacacaagaa gctcaattta ctatagataa acataaggct caagaatatg gtgtaaatac
     8581 cgctgaaatt gcaggcatgt tacgtacagc aataaatggt gactccgtaa ccactgctac
     8641 tattgatgat tacaaagtgg atgtaaacct aaaatttaaa gcagatacta ttgatagctt
     8701 agaagatata aagcaactaa aagtaaactc tgcaactgga aaagaagttc ctcttggtgc
     8761 tttttcagat ataaaaatgg cagatggtct acaatctata tctaggactg atggagattt
     8821 ttctgtaaat ataactgcta cacttgacaa tttagatgct ggtactgcta caaaactagc
     8881 actagaaaaa acgaataaaa tggtaattcc taaagattac tctataggtg ttggtggtga
     8941 aacagaaatg atgaatgaat ctatggcaag tcttggattt tcaatggtca tagctatagc
     9001 attagtttat atggtaatgg tagctcagtt tgaatcattt agtaaaccat ttataatcat
     9061 gttctgtgtt ccatttgcat tcgttggagt tgtgctttcg cttctagtta gtggtcaaag
     9121 tttaagtact gtcggtatgc taggtataat cttacttatc ggtattgttg ttaataatgg
     9181 tatagttctt attgactaca tagagcaact tagaaaaaca aatcgtaaca atgatttaat
     9241 tggtttggtt tcaattggtt gtgcagcaag attaagacca gtcttaatga ctacagcaac
     9301 aacaatttta ggtatgttgc caacagcact atctcttggt gaaggtggag aaactatgca
     9361 accactagcc atagttataa tgggtggtct aactgtatct acacttataa ctttggtgct
     9421 tatacctact atatacttaa tatttgataa atgggaaaat aagttttcag acaaaatgcc
     9481 taaaatattt ggtaagataa cagataaaat aggaagtata aaacgtaaaa taataaagaa
     9541 accaaaattg aaagctaaag tttaaataga atggagggtg attgtattga ataaaaaaat
     9601 gagcaaaata gttgcaattg gctgtggtgt tggtataata actgccaatt taatcccagt
     9661 ttacgcagat aataatactt cttctataaa caaagttgat aattctattt taagtgactc
     9721 aaataaaaga acatctgaaa aggaaatttt gactgctaga gaagctgtta gtgctgcact
     9781 taaaaatagt gaaaaattaa aaatgaaatc agaagaaata aaaatgttaa aagaaaagtt
     9841 agaagtacaa gatgagtttg atagttttac tggaagtgac aactcgtttc catacgacca
     9901 aattggactt cttaaaaatc aaagtgaaca ggctaaaggc tttatggagg accaaatagc
     9961 aaatgatatt actaataaat ttaacgattt agtttcaaga gaaaatgagc tagacaagat
    10021 aaaaaacaat cttgaaataa aaactaaaga aattaaagat atgaaattaa aaaaagattt
    10081 agggttagta acatctttag aaactgaatc agctgaactt gaacttcaaa cactacaaaa
    10141 tactcaaaaa gctaaacttc aagaactaaa aaacaatcaa gactacttta agttgttgac
    10201 taatatagat ttaaataatt atcaattgga taaagaatat agatttgaat cttttagagt
    10261 aggtggctca gttgattcat atatggaagg taaggttaat gaatatttaa aatacgacca
    10321 attgatttta gaacgtacag aagaaagctt taatgataaa gatgagaaca aagcagattt
    10381 accagataga cctaatctta caagaccagt tgctcctaca aaaccagagc aaggtaacct
    10441 aactgatgat gaatataagg ttcttacgga taagtatgaa aaagactaca agagatatga
    10501 aaaagaagtt gaagcataca atcttgaaag acagacttat gctgctggcc ttactactta
    10561 tgcaaattat cttcaacaaa aatttaatac cgaaaatggt ctagtaacat tagaagacag
    10621 taaaaaagct cttaaaaaag ggttaattga tagctatgct caattacttg ctttagaaga
    10681 tacaattcag attactaaaa aacaacttga tttatcagaa aaacaattaa aaaatactaa
    10741 attaagatat gatttaggat taattactct aactgattat aaaaaacaag tggtaagtaa
    10801 tgaagatgct aaaaatagct atgacactct tattgtgaat tataactcac taaaaaatgg
    10861 tatagaaaaa ccttggatac ttaatactgg aaaataattc tggtaaatag aaagattact
    10921 ttaacatacg tttgattaaa aaatagttat gcacactcgt ggctttagtc atggaagatt
    10981 cagaaggcaa ataatagaaa tagattagta aaaatatatt tgcttataaa actgtataat
    11041 tttatagagg ctatctcaaa atagagataa gcctcttttt tattgtttaa attataaaaa
    11101 tatatttaat tccatacaaa aagagtgcct tatgaactaa aaagttcatt ttgagacacc
    11161 ctcaaaagct taactctaaa tttaattatt ttttatcaaa tgtctcatct attttacatc
    11221 tcaccatgtt aacaaattca gaaatattat atcctctaag tttgcatcca catttaggac
    11281 aataatccaa tttcctagat accaaagccc tacaactagg ttctggacat cttattttta
    11341 gactctcatc tgctacttta aatccacatc gtgggcatat actacctgga cgtgatgata
    11401 cttcctttcc acatttagga cactttgtca actttatccc ctccagtaaa ctcaatttaa
    11461 ttgatagttg aaatttctct ctacaataat atatgcaata taattaaata cgtttcactt
    11521 acaactatat aagttaaatt tttataataa aaatccccta tattcaattt acaaatactt
    11581 aatttgatac attgaacaat aggggatttt atttttattt agttacatat tttgcagtct
    11641 attattatct aggatttttt atagatgctt gagctgctgc aagtctagca attggcactc
    11701 taaatggtga acaagataca tagttaagtc ctacactata acagaactct actgaagatg
    11761 gttctcctcc atgctctcca catattccta gttttaattc tggtttaact tctctaccta
    11821 atttagctcc catcttaact aacttaccaa caccattttg gtctagtact tggaatgggt
    11881 ctttttctaa tatttcttca tctacatatt gacctaagaa tttaccagca tcatctcttg
    11941 aatatccaaa tgccatttga gttaagtcat tagtaccaaa actgaagaag tctgcttctt
    12001 gtgctatttc atcagcagtt aaacaagctc taggtatttc tatcatagta cctacagtat
    12061 aaggaacttc tataccttct tcttttatta tagcgtcaac agtttctact atcatttttc
    12121 ttattatttt taactcattt acttctccaa ctaatggaac cattatctct ggagaaactt
    12181 taacacctgc tttgattgct tctatagcac cttgtattat agctttagat tgcattacac
    12241 atatttcagg ataagttatt gcaagtctac aacctctgtg tccaagcatt gggttaaact
    12301 catctaaatc aactattctc ttcttaatat cacttacttt tattcccata gattttgaaa
    12361 gttcttctat agtctcatca tcatgaggca aaaattcatg tagtggtggg tctagtaatc
    12421 ttatattaac tggttttcca tccataactt taaataactc tacaaaatct tctctttgca
    12481 ttggtaatat tttttcaaga gctttttctc tttgttctac tgtattagat aatatcattt
    12541 ctctaactgc tggcaatcta gcttcatcaa agaacatatg ctcagttcta catagaccta
    12601 taccttctgc accaaactct atagctgcac gagcatctct aggattatct gcattagttc
    12661 taaccatcat acatttgtgt ttgtctaccc agttcattaa tttttcaaaa tttccagtta
    12721 aagatacttc tgttttctta acatctccta ggtatacatt tcctgtagaa ccatctatag
    12781 aaatgtattc tccttctttt ataactacat catcaagtgc tcttatttct ttattaaact
    12841 catcaacttt tatttctcca catccagcaa cacaacattt acccatacct ctagcaacaa
    12901 ctgctgcatg tgatgtcatt ccacctctag ctgtaaggat tccttctgct ttaaccattc
    12961 cttctatatc ttctggagat gtctctagtc taactaaaac tactttttct cctttttcat
    13021 ttgcttttac tacatcatca gcattgaaat aaactttacc acttgctgca cctggtgatg
    13081 ctggtaaacc ttttgctata accttagctt cttttaatgc cttatcttca aattttggat
    13141 gtaataattg gtctaattga ttaggctcaa ttctcatgat agcttctttt tcatcaatta
    13201 ttccagcttc aactaaatca accgctacat ttattgctgc tgctgctgtt ctcttaccat
    13261 ttctagtttg tagtatatat aatctttcat tttctatagt aaactctata tcttgcatat
    13321 ctttataatg accttcaagt atatgagtaa tttttacaaa ttcatcgaat acagctggca
    13381 ttacatctgc aagagttgat atattttgag gagttcttat acctgcaaca acgtcttcac
    13441 cttgtgcatt cattaaaaat tcaccaaata atttattttc tcctgtagct ggatttctag
    13501 taaatgcaac acctgtacca ctagtttctc ccatattacc aaataccatt gattgaatat
    13561 ttactgctgt tcctaagtta tgagctatat cattcaattt acggtatacg attgcacgag
    13621 gattattcca tgatctaaat acagcctcta ttgcaagcat taattgctct tttgggtctt
    13681 gtgggaaatc attttttatt tcttttttat aaattgcttt aaattctttt actatttctt
    13741 ttaaatcatc agttgtaagt tctgtatcaa ctgtgtatcc ctttgcttct tttactctgt
    13801 ctaatacatt ttcaaattta tattttggaa cttccatagc aacatcagaa aacatttgta
    13861 tgaatcttct gtaactatca tatgcaaatc tttcattttg tgtagcctct gctaagccaa
    13921 caacactatt gtcattaaga cctaggttaa gaattgtatc catcattcct ggcatagaga
    13981 atactgcacc agaacgaact gatactaata atggattctt attgcatcct aatgttttat
    14041 ttaagtcttt ttctaaagta gctaaatgct cttctatttc ttttattatc tctgctctta
    14101 tactttcatt gttaacataa taatcattac atgcttctgt tgttatagtg aatccaggag
    14161 ggacaggtaa gcctatctta gtcatttctg ctaaattggc acctttacct cctagtaaag
    14221 atttcatgtc tttgcttcct tcaccaaaac tataaacata cttagtttcc attgttaaat
    14281 cccccattaa tctttttatt ttaatttata tcatttttat taatagacat tattaaattt
    14341 ctatttcttt gtatatcttt agaccatttt ctttcattat gtctaaaatt atacctgctg
    14401 tttcttctat tgccttattt gaaacgttta ttactgggca tccaattctt ttcattatct
    14461 cttctgaata atctaactcc tgaagtattc tttctaaatt agcatagttt gctttactgg
    14521 acaatcctaa agcttttaat ctttgggttc ttatctcatt cagcttttca ggtgaattag
    14581 taagacctat tatcttttta gtatctatct caaacacttc tttaggaatt ggtatttctg
    14641 gaactaatgg aacattagct acttttatat ttttattagc taaatacata ctcaaaggag
    14701 ttttagatgt tcttgatatt cccactaaaa ttatgtctgc ttgaagaaca cctctagggt
    14761 cttttccatc atcatatttc actgcaaatt ctatagcttc aactctctta aagtaagatt
    14821 catctaattt tctaattata ccagcttctc ttttaggttt tcttcctgtt ctcttgctta
    14881 tttctcttag tatatctgtc attaaatcta tacaactaag accttctctt tcgcaatatc
    14941 tttcaacaat actgcataat tcttcatcaa ctaatgtata tacaattaaa gcgttttcag
    15001 cttttgcatt ctccaaagtt tcaagtagta ctcctttatt aatcatgtat ggaaatcttt
    15061 ttatttcata agtttcattc acataaaact gagacataca tgcctttgca acttgttgtg
    15121 cagtctcccc tactgaatct gatactgcat atataattag gtttttcacc agcatacctc
    15181 cttaattgtc aactatttct aagaatagct tagttatgtt ggtttttgat attctcccaa
    15241 caactctcat atgatttttg tcttctccct tatattcaac aatcggaatc gaatctactt
    15301 catgttctat tatttttctt gaagctaata aaacacttgc tcctttagtt aaagtgacta
    15361 catttggagt tcttgtcatt atcataccta ttggcatttt atttatatca gaacctccta
    15421 tagttgcttt tagcaagtct tttctggaaa caactccaca taactcttca ttctcatcta
    15481 taataactat actccctaca tcagataaaa acatagtgac tattacatca tatatacttt
    15541 catccttctt agctagtaca ggcatgctca ttatatcctc tactgttttt tcttttacac
    15601 ttttaccaat taaatttatt tcactaactc ctgaataaaa atatccaact tttggtcttg
    15661 cgtctaatat tcctgtcatt gttaatatag ctaaatcaga tctaagtgta gcacgagtca
    15721 cattcaagct ggaggcaata ctttcactcg ttattggttc attttcctta actatatcta
    15781 taatttttag ttgtctttca ttaagctgaa taataatcac cttctttcat tgtgctatac
    15841 tttttatctt ttcgcatctt atatagtata ttatataact atatatacca ttctgtaaac
    15901 agatttttta ttgaatacga tatatttttt actaagttct ctcgcttaaa gatatttatt
    15961 atatattaat gggggatatt tctttaatta atagaactat attagtatta cagttaatat
    16021 tataatacat aattggtcat tttaattcat tttatattaa aatttttaat aatattattt
    16081 caatgtgaat ataaaattgc agaactaatt caaaataatt tttacgacta caaataacct
    16141 ataatatact tttaaagaaa gtatcctaac aagaatatat aaaaatttaa aagggttgat
    16201 ttaaaaataa ccatcaaccc tttttactaa tatctattta tattaccatc aaatcttacc
    16261 caaatatttt ttcttgcaat tgtcttaaat gatataaagt agtatctttt tctatctcta
    16321 ccatatcgta agttataact tcaccttttt taatatcttt tacaacttta gtctttttac
    16381 ttatcaaacc aataggtaca gcattcatag cctttgctac atcgtatttt tctatagtac
    16441 catagcatgt atatgctcca tagccatcca taaactcacc ttttttcaaa tctttcttag
    16501 ctatagtcat aacttctgct actagtccag ctttaggtac tattgtagcc ttatggtcta
    16561 aaactgccat agctgctgat aaaggagtct ctaaactaca taaatgatat ggtctatata
    16621 atatgtaatt aggtccatca cccatactta aatattttag ttcatcatta actgctttta
    16681 atttatgggc aactactacg aaaactccag gtgctatacc atttacaaaa tcaactacac
    16741 cataattatc taatactcca ccttcgcttt ttaatgcata tttcttaggt acttcattaa
    16801 ctgtagcttc aataccatga ccacctctta catcaggcac aaatccagtt gcattacaca
    16861 ttaaagccat ttctaccatt gttttagttc cttctttaaa agaagctatc atatgaggtg
    16921 aagctccttt tctttctgct tcttctctaa ctgtatcagg attacaatct aaatctaatt
    16981 tattattttt acctttacct atcactctaa cttcaaaccc cattgcatct gcaaaatcaa
    17041 ataattccat tactgcacct ggctcatctc ctgcagaacc tgtaaatatt actccagcat
    17101 catctgctaa ttttttaagt attggtccaa caacacaatc agtttcagca tttaacataa
    17161 ctatatgttt tttatttaaa atagagttta atgctatctc tgcaccaatt gcaatacccc
    17221 cagttgcatc tataactaca tctattgact tagttttaca agccacaaga aagtcattac
    17281 atgtcaatac ttttccttct tgtaatttta tttctgcatc cttttcattt gttacattat
    17341 ctataatttc ttcatctatt cctgcatcag taaaagcttt atgagccaac tccaaatcta
    17401 tatctacaac aatagaagct tcaatacctt tcatatttgc catttggctt accattccat
    17461 ttcccatatg cccagctcct ataagagcta cttttatttt tttaccttcc tcttccaatt
    17521 ttgcaagttt tctgtttaaa tctaacatac caattcctcc tgttataata attttatttt
    17581 caaattaaga aatagttatt aaatctccaa tagttatatc tggatttcct atttccacat
    17641 gtatttctcc tggtaaattt acttcacatt ttccatcaaa tttgattgta caatgcccaa
    17701 tttctctaag agtcttctga gcaatatcac caactgaaac tatacaataa tctctattcc
    17761 ctatagtaag tgtatctcct atttcaacat cactttttaa tgttgctatt gaatgaatta
    17821 cagatatatc ctttaagtcg tcatcattaa tggtttcatc atatataata agacaattgc
    17881 tttcatccat taactcttta actaaactgc ctatcccact tatcttaact tcatatttca
    17941 tgacattcct cctgataatt tatatttata ttgagttaat taaaagaaac atttattttt
    18001 tcttttataa attttatagc ttgtacaata ctctcttcat ctttaaattt ttgctctgca
    18061 ctatatatat ctaatccttc tatttcttca atctctttaa atttagccat tactgtacgc
    18121 ccttttaaaa tttctccaaa ctttataaac ccatctgaat ctgaagatat aacggctata
    18181 cttcctttag aaaaatagct tttctttttg cctgtagaca ctatatcacc ctttttttta
    18241 gctctattta aagttttaaa atacaattta ttctgcttca ttataaatat agtatttaaa
    18301 atataagcaa caaataaaat tactaataat ttcatattta tacaccttcc aaaaatcaca
    18361 ttatttttaa atctgtataa caactataaa tgctgtacaa tagttatata aggtttttaa
    18421 gtaacacctt tttaaattct aaaaaagttc tacatttact tatcttattc gttaatttta
    18481 catcttggcc tatctttaaa acttcttcat aaatttttac taataagtct gagtctatct
    18541 tattctcaca tggaaacatt gtcataagaa ttatttttat acttctattt gcatattcta
    18601 ttggttcttc taaaattcca actgccatta aaaacttatc tgatttttca tttatagaat
    18661 gaggaaataa taaaccttta tcaaatactg tgggagattt ttcttctcta ttgactatat
    18721 tttttctaaa cttctcatca atacatccca aatccataag atggtccatc attttttcta
    18781 atgaggcaag tatagtcttt tcatttaata tcataaattt atcttcatct aacaaattat
    18841 ttattagtaa atttgtagaa ttagtagtgt caatattccc ttctcttagg cacaatactg
    18901 tttttaattg ttctcttagt ttgttttcat caaatattgt atttattttt aatactattg
    18961 tatttatatt tgatgtgtct aaatcaatag ttgtaaaaac tatatcgtat tcatctaagt
    19021 taatatttct caaattaaaa cttgaaagag tatcaatttt aggattattt cctatcagtt
    19081 tttctactct tattttcaat agttttgctg ttcctagacc tgtactacat ataattgcta
    19141 ttttttttat tgaatagaat ctactgctat ttctttctaa atatccactg aaatggattg
    19201 ctatatatcc aatctcatct tctgaaactt ctaagttata ctttttctct attacttttc
    19261 ctgctatttg tgctaattta aatggaagta tataattttg cttaatctct tctaacaaaa
    19321 cattttttaa ctttatatta aataatagcc tgtttaaagt aaaactaaga tgatatccta
    19381 gacctactct taattcatcc tcatttataa aaatacccat atcgacatat atttgattaa
    19441 atatattttc catcaactca tcaatgtttt tactaattct actactattt aaagcacatt
    19501 ctgatgctgg tgcatttcca ctaactaacg gtatagttaa ataaataatt tcttcatcat
    19561 ttaaagtaca ttcaaacatt cctgatatta tatgttttat ttccctagca ataaagtatt
    19621 catcatatga tttaagtaaa tcaaacttta catcataatc ttttactttc ttttcttctt
    19681 ttactctata gactaaaact actatgtagt taataacttc tcttaacatt tcatctgtaa
    19741 cataaaaatt atttttttta aataaagtca atagtttttc ttctaaattt atgatttctt
    19801 cttttttgat tcctttaaaa tactttgaat tactgaaatc tttccttgag tttttataca
    19861 actgatttaa tataaacagt ctaatatcaa gttcatttcc atttaatttc atacctgtat
    19921 tctgtttttt aatcaagttt aaattatatg aagccaaaac tttttctaca tactggaaat
    19981 catttactaa agtagttctt cctacacaca tctcatcagc taaactatca agtgtaataa
    20041 atccatctag ttctatcaag taatttagta taaactctac tctatcttct tttgaattta
    20101 atgaaaatac attttttcta ttcatatcaa taatcttatt gagtatctgc tcatctttta
    20161 tttctagttt atagccttga ccctttatat atttgattac tgctatatca ttacctaaat
    20221 ctttattcaa ttgttttata tacgtcctta cacttctagt tgtgacattc aattcatttg
    20281 ctattgagtt aatactcaca aactcttttt tactacacat aatcaatatc ttaaatatct
    20341 tgcggtcaat agaaggaatt ttctcagtta cactcatttt gtatctcctc cttgatttca
    20401 ttctagtaca aatttgaaac caaattaata cataaaattt cactttaata aaggaaaatt
    20461 tagtattaat ctaactcgat aattacagct aattaatagt ataaaagcta gtctttcatt
    20521 aacaaaaaat cttgactagc ttaaatttat atttacaatt ttattaatta attccaagcc
    20581 ttatattgac attaaatatt atctcaatgc aaatactgca actccatatg caataataac
    20641 agctattgga ccagttatta aacgagagta aaataaagct ggaacaccat actctacagt
    20701 ttctggttct gcttctccca aacttaatcc tactggtaca aaatcacatc cagcttgccc
    20761 atttattgca aataaagctg gaagtgctaa atatgctggt attgcaccaa gaccaatttg
    20821 tgtacctaca agagtcccta ctacttgagc tataactgca ccaggtccta gtattggtga
    20881 taagaatgga agtgtgcaaa ttactgatat aatcaataat ccaccaatat tacctgcaag
    20941 tggagatata actcttgcaa tccagtctcc cagtccagat gctaaaataa ttcccataag
    21001 catacttaca aatgccataa atggtattac gttattcatt accatttgga tagtatctcg
    21061 acctgcatca taaaattttg atactacttg ccctgcccct tgaccaatag aaagtattat
    21121 gttcttttta gcatattcac cttgtgcttt tgacttaacc tcatcataat tatcaggctt
    21181 tctaatagaa ctcttatttt cgcttttatt ttcttgagat ttttgtggca tatcttcccc
    21241 atctacaaca cttatacagt taggattaac atctgataca taatattctt ctgttataaa
    21301 ttttgctaaa ggacctgttt ttccaactgg atttacattt attgtaggta tttttttctt
    21361 tggataaact ccacatctag cagttccacc acaatcaata ataactactg ctacttcttc
    21421 atcagaaact ccagttttaa atccatctac aatttcacaa cccagtagtt cttttatttt
    21481 taatgttact tcatggatac cgccagcagt cattgatagt atcttatttc tattcccttc
    21541 tttttttata tacaaaggac ctccccagcc tgaagttccc ttttctattt ttaatatttt
    21601 attctcctcc attttatcta cctcctaagc cttatttgtt gctctatttt ttcttgttag
    21661 aaaagcatac attttttctg ttactatacc tcttataaaa attacaatta gaccaactat
    21721 aaaataccac aatgccaatg tagtagtttc tttacctaaa gtagtcaaac ctgcagatat
    21781 tcccaaccat acaaaaagtt ctcctgcatt tgcatgtgga aaaagacctg taattggatg
    21841 tacaaatgaa actgctgcat cataaaatcc tgctttgtgc tcttctttta aaaatctacc
    21901 aaatgtatag cacataggat ttcccaagaa aaatactgat actaatggaa gtagaatata
    21961 tcttgtaaaa gagttctttc ctagcttaga agcccaatta ttaactcttt cctcaccaat
    22021 tatcttaatc aaagagttaa tagctaccat taatacaatt agcgttggaa gagtacctga
    22081 aacaagacct ataaattgtt ctcccccttt ctgaaacaaa ccgataaacc actctgcacc
    22141 tttacttaga cctgtaataa cgttttccat aaaattataa cctccctata atatctttaa
    22201 atttttaatt ttactgctta ccataaattt tacaaattac atctttt
//
LOCUS       DAYASV010000058        22058 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYASV010000058 DAYASV010000000
VERSION     DAYASV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.058"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(269..460)
                     /locus_tag="ACGI68_002109"
     CDS             complement(269..460)
                     /locus_tag="ACGI68_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138544.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            680..1726
                     /gene="cobT"
                     /locus_tag="ACGI68_002110"
     CDS             680..1726
                     /gene="cobT"
                     /locus_tag="ACGI68_002110"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFH0138545.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            1758..2318
                     /gene="cobU"
                     /locus_tag="ACGI68_002111"
     CDS             1758..2318
                     /gene="cobU"
                     /locus_tag="ACGI68_002111"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFH0138546.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            2328..3101
                     /gene="cobS"
                     /locus_tag="ACGI68_002112"
     CDS             2328..3101
                     /gene="cobS"
                     /locus_tag="ACGI68_002112"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFH0138547.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            3121..3735
                     /locus_tag="ACGI68_002113"
     CDS             3121..3735
                     /locus_tag="ACGI68_002113"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFH0138548.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENNSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      3802..3981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4096..5610
                     /locus_tag="ACGI68_002114"
     CDS             4096..5610
                     /locus_tag="ACGI68_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HFH0138549.1"
                     /translation="MGKKIMLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMAL
                     NSFITKEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSS
                     KYHDYKLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPV
                     IIVGDIDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKV
                     PVIGVVPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVS
                     IRYVDYGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQ
                     MLGKKLKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGK
                     KVKGYEIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEIS
                     FTRAILNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            5649..7025
                     /locus_tag="ACGI68_002115"
     CDS             5649..7025
                     /locus_tag="ACGI68_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HFH0138550.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            7026..7997
                     /gene="cbiB"
                     /locus_tag="ACGI68_002116"
     CDS             7026..7997
                     /gene="cbiB"
                     /locus_tag="ACGI68_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HFH0138551.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKFGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            8029..9099
                     /locus_tag="ACGI68_002117"
     CDS             8029..9099
                     /locus_tag="ACGI68_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0138552.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEERKYML
                     QELRNIRNIKVYDTDANFILIRIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            9101..9988
                     /locus_tag="ACGI68_002118"
     CDS             9101..9988
                     /locus_tag="ACGI68_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFH0138553.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            9976..10608
                     /locus_tag="ACGI68_002119"
     CDS             9976..10608
                     /locus_tag="ACGI68_002119"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HFH0138554.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            10617..11843
                     /gene="cbiD"
                     /locus_tag="ACGI68_002120"
     CDS             10617..11843
                     /gene="cbiD"
                     /locus_tag="ACGI68_002120"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HFH0138555.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDNLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            11840..12448
                     /locus_tag="ACGI68_002121"
     CDS             11840..12448
                     /locus_tag="ACGI68_002121"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HFH0138556.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            12438..13013
                     /locus_tag="ACGI68_002122"
     CDS             12438..13013
                     /locus_tag="ACGI68_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HFH0138557.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            13006..13758
                     /locus_tag="ACGI68_002123"
     CDS             13006..13758
                     /locus_tag="ACGI68_002123"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HFH0138558.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            13742..14863
                     /gene="cbiG"
                     /locus_tag="ACGI68_002124"
     CDS             13742..14863
                     /gene="cbiG"
                     /locus_tag="ACGI68_002124"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HFH0138559.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYQVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYVDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTQKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            14860..15585
                     /gene="cobJ"
                     /locus_tag="ACGI68_002125"
     CDS             14860..15585
                     /gene="cobJ"
                     /locus_tag="ACGI68_002125"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HFH0138560.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     GKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            15582..16334
                     /gene="cobK"
                     /locus_tag="ACGI68_002126"
     CDS             15582..16334
                     /gene="cobK"
                     /locus_tag="ACGI68_002126"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HFH0138561.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            16353..17831
                     /locus_tag="ACGI68_002127"
     CDS             16353..17831
                     /locus_tag="ACGI68_002127"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HFH0138562.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            17861..18766
                     /gene="hemC"
                     /locus_tag="ACGI68_002128"
     CDS             17861..18766
                     /gene="hemC"
                     /locus_tag="ACGI68_002128"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFH0138563.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSTIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            18756..20255
                     /gene="cobA"
                     /locus_tag="ACGI68_002129"
     CDS             18756..20255
                     /gene="cobA"
                     /locus_tag="ACGI68_002129"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFH0138564.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            20272..21237
                     /gene="hemB"
                     /locus_tag="ACGI68_002130"
     CDS             20272..21237
                     /gene="hemB"
                     /locus_tag="ACGI68_002130"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFH0138565.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            21390..21968
                     /locus_tag="ACGI68_002131"
     CDS             21390..21968
                     /locus_tag="ACGI68_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HFH0138566.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT         9075 a         2125 c         4159 g         6699 t
ORIGIN      
        1 tttgggatta tttaagaaat tttgtattat aaatagaagg aaatagataa attgtataga
       61 ataaaatata ttaagataag ttaataatgt taaatgggaa gtgagtttat ggaaaaacca
      121 aatacaaaag ttgttaactt tcaagagtat aaagacagaa aaaaccaaag agaagtaaat
      181 agagacgaac ttttaaagct tataaaaaaa attgtgactt caaaataggg agttgttaaa
      241 ctccctattt taatgggttt cataataatt aagacattct aagtccatct atataatctt
      301 tgaattgtga gaaatctgat tcactactta attctatggt ttctgaacct tccgtataaa
      361 aattcttaag atagatagta taattattat caacacaatc atgataaata catctatatc
      421 catcttcata caaacttttt aacttagaaa aatcatccac aatatcatct ccttaaagtt
      481 cttttaaata tatttttaca aaaagcatct aattgtatgc aaattgacaa tgtaaaataa
      541 aggaaatgaa cataaaatgt agaataattt ttatatgtat gtaaaaataa aaagaaaaag
      601 ttgaaatttt tagaaaataa gagtattttt tatctaaaaa gagagtttaa aaaataaaaa
      661 aatgttggga ggataaaaaa tgagtttatt agaaagtata tcaaaaaaca tatattccct
      721 agataactct tctattgaaa aaacaaagca gagattggat aggcttatac atccaactgg
      781 aagcttagga aagatagaag atatttgcat gcaattagca ggaatatttg gcaatgaaaa
      841 ttttgacaca agtaaaaagg ttataattgc ttttgcggga gaccatggtg tatatgaaga
      901 aggcgtagca cctgaccctc aaaatataac aaaattacag tttccaaatt tttcgaaagg
      961 tctatgcgga gttggagtaa taagtaaatt tgtaggggca gatgttgtag ctgtagatgt
     1021 aggaataaac tgtgatgaaa aactggatgg ggtattagac tacaagataa gaaaagggac
     1081 atcaaatatg gcaaaaggtc cagctatgag taaacaagaa gcaattagat gtttagagat
     1141 aggtattgaa atagcagagc agtgtataga aaaagattat aaggttattg gtattgggga
     1201 gatgggtata gcaaatacta ctccaagtac agcaataata tctgtaattt cagggtgtga
     1261 cccgttagaa gttactggta taggtgcagg tcttaagaaa gaaagactaa agcataaggc
     1321 tgaagtaatt agaaaggcta ttgaaattaa taatccaaat ccaactgatg gagtagatat
     1381 tttatctaaa gtaggagggt ttgaaatagg ctcaatggca ggtgttatat taggttgtag
     1441 tgctaatagg attccagtag taattgatgg gtttatttct tatgcagcag cattaattgc
     1501 atataagata aatccaaaaa ctagagaata tatgatagca tctcatttat cagcagaatc
     1561 aggaactaaa agagcattag acatattaaa attagaccct ctattaaata tggatatgag
     1621 attaggtgag ggaagtggag ctgctttagc atttgatata atagaagctt caaactatac
     1681 ttataaaaat atggcaactt ttgatgaaat tgacatggga agataaatat aaaaatttaa
     1741 tatgtaggag tgttttgatg agtaaaatta ttctagtaac aggaggagct aggtcaggga
     1801 agagtaactt tgcagaaaaa ctatgttttg acagaaataa taatacagct tatatagcaa
     1861 cctctattcc atttgatgat gaaatgaaag atagagtaaa aaaacatcaa gagaatagac
     1921 ctagaaactg gaatacttat gaaatatatg aagatatata ttccataatt aaggagatat
     1981 ataaagaaca tgaaactgtt atactagatt gtgtaacatt attagtaaat aatctaatgt
     2041 ttgaacacaa tctaaatata gaagaatcta gtcaagaaga aataaacaaa ttagaacaat
     2101 acataaaaga acaagttgtc aaacttattg aagaaataga aaaaacaaat ctatactttg
     2161 tatttgtaac gaatgagtta ggtatgggaa ttgttcctgg gaataaactt agtagaattt
     2221 atgctgatat tgtaggtcgt ataaatcaat atatagcttc gaaaagtaat gaagtttatt
     2281 ttgtagttag tggcatagct atgaagataa aggagtagta tgcaattatg aaaagattta
     2341 ttttaatact acagtttttg acaaggatac ctataaaatt aaatgtagga tttgatgatg
     2401 aattttataa gtcaatagta tattttccac ttgtaggttt tgtcataggt atattatcct
     2461 atttaatagg atggatttct atgcttttat ttgaaccatt tatagcttct ataataataa
     2521 ctctagctgg tgtcttaatt actggaggac ttcatataga tggattagga gatacatttg
     2581 atgcaatata tagttatagg gataaagaaa agatgcttga aataatgaaa gattcaagat
     2641 taggtacaaa ttcacttcta gcaattatgt ttgtactttt attaaaggtt ggattcgttt
     2701 acgatataat aagtaataat tcattgtggg ttataatttt tatgccaatg attgcaaggt
     2761 taggagtaat gttattgaca tataaaactg tgacaccaag agaaaaagga atgggaaatt
     2821 tatttatagg aaaattaact acaagtatgt taataactgc tataatatac acattattga
     2881 tagttgctct tattactaaa tttatatttt tattacctaa tatagtgttg attaaagtat
     2941 taggttcgat tatagtggtt tttgtattta taatattatt caaaaaacat atttataaaa
     3001 aaattgacgg agtaacagga gatatattag gctgtggaat agaactttca gagcttgtat
     3061 atttaattta catatatttg ttaattttta tgttttttta aagtaaaaag tggtgataat
     3121 atgataagat taatattaat aagacatgca ctgacaaatg ataataaaaa aggaagatta
     3181 tcaggccata taaatagttg tattagtgaa gagggcaaac tacaaataaa taaaattacc
     3241 agatatctga gtaatgaaaa catagataag gtttatacaa caccatctac acgtactaaa
     3301 gatacagtag aaaaaatatc aaaattgaaa ttattagaaa tagaagaaaa agaagcttta
     3361 agagaaatta gttttggaga ctttgaagga agaacttttg aagaaattaa aatcaaatat
     3421 ccaaatgagt ttgaaaaaat gataaaagaa ggtaacaact atagatatcc aaatggagaa
     3481 agtttgatag actcttataa aagagtggca aaagaaattg ataatataat tttagaaaat
     3541 aatagtaatt tagatacaaa gacaatactt atatgttctc atgcaggaac aataagaaat
     3601 ataataacac atttgatttc tggtagttat aagtatcact ggaactttaa aattgataat
     3661 gcatctatta cagttttaga aattgatggt ggatttgctg taatagataa aatgaatttt
     3721 acagacttca tataatttaa tcaaaaccaa acattacaat ctgtgtttgt aatatatctg
     3781 taaaattagc aatgaaaata gaatattaga ttaaaattag gttcttaatt agatgttaaa
     3841 agggaaaaag gttagatgcc tttgcagccc ccgctactgt gaaaccaacg aaactataaa
     3901 taccactgtc agtttgatgg gaaggttata gaagtaggat gaagttaagc caggatacct
     3961 gcctaattta atttaaaaca agaggtcttc ggggtgaaag atttttaatt aatatatata
     4021 aaagtcctat ctttagaagt attaggactt tttttaattt gttttttagt aaattttaac
     4081 aaggagggat ggaaagtggg gaaaaaaata atgctacaag gaacagcttc aaatgtaggt
     4141 aaaagtgtct tagtagcagg tttgtgtaga atatttaagc aagatggtta tactgttgct
     4201 ccttttaagt cacaaaatat ggctttaaat tcttttataa ctaaagaagg attagaaatg
     4261 ggaagagcac aagtatttca agcagaggct gcgaaaatag agccaatagc tgatatgaat
     4321 ccagttttat taaaaccttc tggaaataat aaaagtcaag ttattgtaag aggtaaagtt
     4381 gtaggagaaa tgccttcttc taagtatcat gattataaat tggagttagt agatgtgttg
     4441 aaggaaacat ttaatagctt aaattcaagg tatgatattg tagttatgga aggggcagga
     4501 agtaatgcag agataaactt aaaagataga gatatctcta atatgggtat ggcagaaata
     4561 gctgatgcac ctgtaataat agtcggagat attgatagag gaggcgtatt tgcctcatta
     4621 gcaggaacaa tgcttttatt aaatgaagat gagaaaagaa gagtaaaagg tgtaataatc
     4681 aataaattca gaggaaaaaa agagctttta caagatggtg taaagatgct agaagacatc
     4741 ataaaagtac ctgttatagg agtagtacct tatacagata taaaaataga agatgaagac
     4801 agtgtaacta cgagatttaa gaaaaaaatg aataaaagtg atatacacat agaaataatt
     4861 agaaccccac atatgtcaaa ttttacagat tttaatatat ttgagacaca agaagatgta
     4921 agtataagat atgtagacta tggagaatct ttaggaaatc cagatatatt aataatacct
     4981 ggaactaaaa gtactataga cgatttaaca tatataagaa aaagtggtct tgaatcgcaa
     5041 atcaaaaatt tacatagtca aggtaaattg atatttggga tatgtggtgg ataccaaatg
     5101 ttaggtaaaa aattaaaaga cccttatcat gttgaaagtg aaattgaaga agttgatgga
     5161 ataggtcttt tagatacaga aacaacgttt gagaaagaaa agactacaac acaagtagaa
     5221 gctactattg ttgaatgtat cagtgaatac atgaataacc ttaagggaaa aaaagtaaaa
     5281 ggttatgaaa ttcatatggg aattacaaag cgaggtaatg atgtaagaag tttagattta
     5341 ataactaaaa aacttgataa agaagtgaat tatactgagg gaagtgtaaa taaagagggg
     5401 aatgtaattg ggacttatct tcatggaata tttgatgaaa taagctttac aagagcaatt
     5461 ttaaacaata taagaaagaa aaaaggttta ggagaattag aaagcaaagt aaactctttt
     5521 aatgaattta aggataaaga gtatgataag ttggctgatt ttttaagaga acatttagat
     5581 atagagaaaa tatatgagat tatgaaataa tgatgacctt tatttataat ttaggtagga
     5641 ggtgtattat gaaaaaaatt ttaattgcgg gtactaatag tggagtaggt aaaacaacaa
     5701 tatcattagg aataatgcaa gctttggtta agagaaatat gaaagtacag ccatataaag
     5761 tagggccaga ctatatagac ccttcatatc acacttttat aactggaaga cattctagaa
     5821 atttagattc ttatatgtta gatgatgaaa aaataaagta tatagtaaat aagtcctcaa
     5881 aagatgcaga tatttcagta atagaaggtg ttatgggctt atatgatggc tttggaatag
     5941 atttagataa ttgtacaagt tcacatactt caaaggtatt aaagtcacct gttatattag
     6001 ttataaatgg taaatctatg gcagcatcta gtgctgctat ggtacttgga tataaggaac
     6061 tagataaaga tgtaaatata aagggtgtta tagtaaataa tgtaaagact aatagccatt
     6121 atcaaataat taagagttct atagaaaaat attgtaatgt tgaagtattg gggtattttc
     6181 ctccaaataa caaattctct ttggaatctc gacatctagg attagttcct agtgtagaaa
     6241 tgaaatcact aagggaaaaa ttttatacct tagcagatga aattgaacac tatatagata
     6301 ttgacagaat aattgaaata tcagaaagtg atgaatttga gagtgatttt gattttcaaa
     6361 ctttgataga aaataataaa ataaaagaac atgttaataa taaatcaata gctatagcat
     6421 atgataaagc attcaatttt tattatagag aaaatataga gcttttagag gaacttggtt
     6481 tagaaataaa ctattttagc cctcttgaag atgtatcagt gccaaatgca gattatattt
     6541 atattggagg aggttttcca gaaatatttg gaaaagaatt agaagcaaat gagtcaatga
     6601 gaacttccat attagaagca cataacaata atatacctat ttatgcagaa tgtggaggac
     6661 tgatgtacct aggtgagaaa ctattgaatc aagaagaaca agaatttaat atggttggaa
     6721 tatttagtgg aattagtaag atgacatctt ctttaaagag atttgggtac tgtattggtg
     6781 tagccaagga aaatactctt ttatctaaaa aaggggaatc cattaaaggg catgagtttc
     6841 atcattcaat atttgaaagt gatgaaaaat gtgtatattc aatgagaaaa gaaagagatg
     6901 gaaatatagt agaagagtgg gatggtggtt atagtaaagg aaacactttg gctacatatc
     6961 ttcatactca tttttataat aacttaaatt gtatagaaaa ttttttgaaa aaaggaaatg
     7021 aataaatgag tattttatct atttatatag gatatgtgac agacttaatt gtaggagacc
     7081 catattcttt tcctcatcca gtgagattta ttggtaaact tataaatttt actcaaggta
     7141 taataagaaa ggtctctaaa aatgataggc agttaaaatt tgggggattt atattatggt
     7201 ttataaccgt tggaataaca tatttaacaa cttatgctat agtgaagtta tttagtttta
     7261 atatattatt gagtattgta gtgaatagtt ttataattta tacaacacta gccacaaaat
     7321 gtttaaaaga cgaagcatta aaaatttata atgtattaaa aactgaagat atagaaaaat
     7381 ctaggattca gctatcttat atagtaggta gggatactac taatttaagt gaacctgaaa
     7441 taataagagc aactgtagaa actgttgcag aaaatactgt agatggtata attgcgccta
     7501 tgttttatgc atttattgga ggagcaccac ttgctatggc ctataaagct atcaatacat
     7561 tggactcaac agttggatat aaaaataaaa agtataaaga tattggtttt gcatctgcta
     7621 agattgatga tatagccaat tatataccag ctagaatatc tgtcattctt atgacaattg
     7681 gaagtttctt tttaaattat aactatagga actgttttaa aatctctatt agagatagaa
     7741 agaatcataa aagtccaaat tgtgcttttt cagaaggtgc agtatctggt gctctaggaa
     7801 tacaactagg aggtaccaac gtttattttg gggaaaaagt atataagcca acaatagggg
     7861 ataagcttag ggagatagat aaagaagata taattaaaac taataaaatt atgtatgcat
     7921 cttcatttgt atctatgttg gtatttacac taatatatgt gttatgtagg atgattttaa
     7981 ttaacttaat tatataaatt attgtttgtt aacttaaggg ggacatagat gaaggattta
     8041 gggcatggtg ccaatgtaga tgaaatggca agattatatg ggaaaaaccc aaaagaaata
     8101 attgatttta gttcaaacat aaatccaaat gtattaccaa atttagagag atacatttta
     8161 aaaggattag aagagtgtag gaattatcca gatataaatt atacaaactt gagagaaaat
     8221 atatcaaaat atatagatat aaatccagac tttataatac ctggcaatgg tgcaacagaa
     8281 gtgatttatt tgcttatgaa gagtattaaa aaaaaattgg ctataataaa tccgactttt
     8341 tcagagtata gaagaagtgc tgagttaaat aatttagata ttatagattt agagttagat
     8401 ttagaaaata actttaaact taatatagat ataataaaag aaaatataaa aagatttgat
     8461 agtttattta tatgtaatcc taataatccg agtggtaatg tccaagattt aaaggaactt
     8521 gttcatttac tagataaaca taataaggta ctaataattg atgaaacttt catggaattt
     8581 gttgaagatg aaagtaaata tagtttggtt aaatatatag aatcaaataa aaatatattt
     8641 ataattaaag ctgtgactaa gttttttggt atgcctggtt taaggctagg atatggtctt
     8701 acaagcaaca ctgaaattat gaacaaaatt tatgaacaca aagagccatg gactatcaat
     8761 tcttttgcag atatactatc caattttatt tttgaagata aagagtatat taaaaatagt
     8821 aaagaatatt acatagaaga aagaaaatat atgttacaag agttgagaaa cataagaaat
     8881 ataaaggtct atgatacaga tgctaatttt atcttgataa gaatatataa aaaaacaact
     8941 aaagaattga aaaaagattt gtttaagcaa ggaaacatat tagttagaga tgcatctaat
     9001 tttataggat tagatgatag ctttataaga gtggctataa aatctcatga agataacaaa
     9061 attcttatag aaaatatgaa aaatttattg ggtgattaaa atgaaatctt atggaatatg
     9121 cccagcatct tgtggagaat ttgtacaagg tataattgat gatgaagaat atttatgttc
     9181 atatgcaata gatatgtatt ctaaagtgta tattgaagaa aaattagtag atataaattt
     9241 agggagatat aagtcaaggt tagcaattga aaaggtattt gaaaaattta atctacctaa
     9301 aaaatatact aaaaatattt ctttaaatat aaatagtaaa atacctgttg gaaaaggaat
     9361 ggcaagttca actgctgata taggagctac tataaaggca acattatcat tgatagataa
     9421 agatttaagt agcgaggaga tttctaaatt agcagcagaa atagaaccaa cagattctat
     9481 atttatagat aaaaatagta tatttaatcc tctaaatgga actgttataa aatacctagg
     9541 aaatctgacc aatgcgaaag ttgtaatact tgaaccaaac aaagtactag atactatgaa
     9601 aattagattg aggcaagatt ataataaatt aaaagtagag aataaagaag taataaaaaa
     9661 atcatttgct cttttagaag aaggattgaa aaaaaataat ttatctctag ttggagaggc
     9721 ttgtactttg agtagcttag caaatgagaa tatagaaaaa aaagagtatt taaatgaaat
     9781 aataaaaata tcaaaaaaat atggagcata tggagtaaat attgctcaca gtggaactgt
     9841 agtaggtatt ttaattgata agtttatgaa tgataaaaag atgatagatg ccttatgtga
     9901 atctaacata aattctgtct ataataaaat ttatacacaa aacataatta atggaggaat
     9961 taagggggaa atagaatgga atacataaag aatcctatga aaatagaaga aaaaagtttt
    10021 gaattgatac aagaaataat agacgaaatt agaccagatt ataaatttaa gaataatatc
    10081 gaagaaaaaa taataaaaag agctatacat actactgctg attttgatta cttagatata
    10141 ttaaaaatat cagaagaagc agttgatagt ataatagatg cattaaaaaa taatgcaagt
    10201 atttatactg atacaaatat ggctcttagt ggtataaata aaagaaagtt agaatcatta
    10261 ggatgtaagt ataaatgttt agtggcagat gatgaaactg taaaattagc taaagaaaaa
    10321 ggaattactc gttctatggc agcagtagaa attgcagcta aagaagaagg aagaaaaata
    10381 ttcgttttag gtaatgctcc aacagcttta tataaagtta tggagatgaa atcagaaggt
    10441 agattagatt tagatgcagt tataggagtt cctgtaggtt ttgtaggagc acaagaatca
    10501 aaagatgaag ttgaaaaaac agatatacca tatataattt ctaaaggtag aaaaggtggc
    10561 agtaatttag cagcagcaat agtaaatgct atattatata ctatgtaggt acctatatgg
    10621 aagaatatgt atatatagat ggaaaaaaat atagaagagg atatactaca ggttcatgtg
    10681 caacaggtgc atctaaggct gctgtatata tgttgattac aaaaaataga atcaatacta
    10741 taaatataga tactcctaag gggataccat tactactaaa agttgataat ataaatattt
    10801 ctgatacatt tgtggaatgt tccataaaaa aagatggtgg agatgatata gatgcaactc
    10861 ataccatgga tatttatgca agagctgaaa ttgtagctaa aaatgataag aacaaagggt
    10921 atcttactct aaaggatata gataatttaa gcaccaatag tgagtgtaaa agtgagctct
    10981 ataagtttat tagggtttat ggcggaactg gtattggtgt ggttacaaaa aaaggtctta
    11041 gcgtggatgt tggtaaacct gctataaatc cgactccact taaaatgata aatcatgaaa
    11101 taagaaagct tataggagat aattttgaat ctatacttgg aaatgataaa gttttaaaaa
    11161 taacaatctt tgctccacaa ggagaaactg tagcaaaaaa aacatttaat ccaagattag
    11221 gtatagttgg aggaatatct ataataggta caactgggat tgttgaacct atgagtgatg
    11281 agggttggaa gaagtctcta tctatagaac ttcagatgaa aaaagaacaa ggcttagata
    11341 aaataatatt ggttccagga aatcatggag aacagtttat aagagaaaaa ttaaacttag
    11401 atattaagta tgtagttagg gtaagtaatt ttataggcta tatgattaaa gaagctcaaa
    11461 gaataggata taagaaaatc ttaatggcag gtcatatagg taaatttata aaagtatctg
    11521 ctggtatttt taatactcat agtaaggtag cagatgctag aagtgagata ttagtggcaa
    11581 acttagcttt aatgggagct agatacgaat ttttaaataa aataaatcaa tgcgtaacta
    11641 ctgaagaagc tgtagagcta ataaataata gtgaatatag agaagtatat aatatattaa
    11701 gtaataaatg tagggagaga gtaaagcaat acttgaatga agattcagat gatattgatg
    11761 tggaagtaat catattttca atggataagt ctttattagg taaatcggat aatacagatg
    11821 atttagtgga ggtctttata tgataaacat aataggtcta ggacctggta acttagacta
    11881 tataacaaaa aaaggtgaaa atttaatttc cacttctgat gtattaattg gtggtaaaag
    11941 aaatttagag tctataaaaa attttgaagg agaaaaaata gttctggatt ctaatttaag
    12001 agaaattata gagtacataa ataacaataa agaaaagcaa atatcaataa tagcatctgg
    12061 ggacccacta atatatggga taggtagata tctatctaaa aatattgata ataagatgtt
    12121 aaacatggtt tctgggatta gttcattaca gtacatattt tcaaaaatat atgtagatat
    12181 gaatgatgta tatattacaa gtagtcatgg taaagtacca gattttgatt acatattatc
    12241 acataaaaaa gtatgtatgg ttacagattc aaagatagga ccaaaacaaa taagtagaga
    12301 aataatagat agaaatctaa ataaaattat tgttgtagga gaaaatctgt catatgacaa
    12361 tgaaaaaata acaatagcaa aaccagaaga gataataaga atggacaatt ttgatatgaa
    12421 tgttgtggtg attttagatg aggaatagtg aatttataac aggtaaagtt ccaattacta
    12481 aggaagaggt tagagcaata tcaataagta agttaaattt agtaaatgct aaaaacctca
    12541 tagacatagg ggctggtaca ggaagtgtaa gtgttgaagc tgcatataat tatcctaatt
    12601 taaaggttat atctatagag aaaaatgatg atgctataga gcttatagag aaaaataaaa
    12661 aaaaattcaa cttgaaaaat atagaagtga taaaaggata tgctccaatt aatctagggt
    12721 taaatgttaa agttgagtct atttttcttg ggggaacagg aaataatctg gaagaaataa
    12781 tagaatggtc taaaaaaaat ctcgttacag gaggaagact tgtagctaat tttataataa
    12841 tagatacatt taatcaaact ttaaaactgc ttaaaaagca tggatttaaa gaaatagatg
    12901 tatgtgtttt gaatgtttct aaactagaga aactaggaaa aggtgaatat tttaaaccac
    12961 taaatcctat atatataata tcttgtgaaa aaggggaaaa tgatgatgaa taaagtacat
    13021 tttgtaggag caggacctgg agataaagag ctgataacat taaaaggata caaattatta
    13081 agtaatgcag atgtagtgat atatgcaggt tcacttgtta atcctgaact tttggaatac
    13141 tgtaaagaag attgccaaat acataatagt gcacatatgg atttacaaga gataatagat
    13201 gttatgagag aaggtataga aaataataaa tctgttgtaa gactgcaaac aggtgatttt
    13261 tctatatatg gttcaattag agaacaagtg gaagatttaa ataaattgaa tatagattat
    13321 gactgtactc ctggagtaag ttctttttta ggggcagcat catctttagg ggttgaatat
    13381 acagttccag aaatttctca aagtgtaatt ataacaagaa tggaaggcag aactccggtt
    13441 ccagagaaag aatcaataca atcatatgct aagcatcaga catctatggt aatattttta
    13501 tctgttcaag aaattgagaa agttgtgtct aaattactgg aaggtggata tccaaaagat
    13561 actcctatag cagttatata caaagcaact tgggcagatg aaaaaatagt taaaggaact
    13621 ttaagtgata tagctgtaaa agttaaagaa aacaacataa ataagactgc tttaataatg
    13681 gttgggagat ttttaggaga agagtataat aattcaaagt tatatgataa ggatttcaaa
    13741 catgaatata gagggtaata ttgctataat ctgtattaca gaaaatggaa aaaaattagc
    13801 tctaaagata aattctctag taaataactg tgttatatat caagtaaaaa ataaaaaaag
    13861 taatttagtt gttgaagaaa catgtgtgca tacagttcaa aagaagttaa gtgattttgt
    13921 aggagaaata tttagcaaat ttgattacat agtatttata atggctacag gtatagttgt
    13981 acgaagtata gcaccataca taataagtaa gtttagtgac cctgcaattt tggtaatgga
    14041 tgaaaaaggc aaaaacgtaa taagtttatt gagtggtcat attggagggg caaacagctt
    14101 gacacagtac ataagtagtc ttattgatgc taatccagta ataacaacag ctactgatat
    14161 aaatcaaaaa gcttccttag atatgatagc aaaaaaacta aatgcacata ttgataattt
    14221 tagagaaagt gtaaaagaag taaattctat gctagtgaat aataaatgtg ttggattata
    14281 tgttgatgaa ggatatcaag ttgatactag gggatttaaa cttatagaaa gtaaggatga
    14341 gttaagagat agtttttcaa acacatatat agaaaaatta gttgtagtaa gcaataaaat
    14401 aaattgtatt gaagatattg agaatttaaa cctagataat aaatttgaac attgctatag
    14461 tacccaaaaa ataataaagg taattccaag agatatagtg ataggaattg gctgtagaag
    14521 aaacacagat agtgaactga tgtataattc acttgaagag tttcttaata aacaaaatat
    14581 agatattaga gcaataaaca cagttggaag tatagaactg aaaaaagacg aaaaagcaat
    14641 aattgaatta tcaaaaatat tgaaggtgcc atttaaaaca ttttcagtag atgaaatatc
    14701 taaggttgat aatctttttg ataaatctga ttttgttaaa aaaaatgtgg gtgtgtattc
    14761 agtagcagaa cctgtagcat atcttttgag caaaggtaat ttgttagttg aaaaacataa
    14821 atataaagga ataacttttt cagtggggag aataaaaata tgatatacgt tgtaggaatt
    14881 ggaccaggaa gtaaagatac aatgacttta gaagcaataa aagcaataga agattctgag
    14941 gctattgttg gatataagac ttatataaaa ctaatagaag aatttataca aggtaaagaa
    15001 ataatccaaa atggtatgag gcaagaagtt gatagatgta aacaagcagt ggaggaagca
    15061 aaaaaaggaa aaaaagtagc agttgtaagc agtggtgatg ctggaatata tgggatggca
    15121 ggtcttattt tagaattaat atctaaagaa gatgaagata taaaagtaaa ggtaattcca
    15181 ggcgttactg caagtattgg tgcagcagct atacttggag ctcctattat gcatgatttc
    15241 tgtcatataa gtttgagtga tttaatgact ccgtgggaag taattgaaaa aagattgaga
    15301 ttggctgctg aagccgattt tgtaatttgt ttgtataatc caagaagtaa gggaagaagt
    15361 gaacatctaa gcaaagcatt caaaataatg ggtgagttta aaggtggaga tacacctgtt
    15421 ggagtggtaa aagatgtagg tagagaaaaa gaagaaaagt ttgtatgtac ttttgaaaat
    15481 atggactttg aaaaagtcga tatgactaca atggttataa taggaaataa gtctacctat
    15541 attaatggtg aattgatgat aactccaaga ggttatacag tatgatttta attttaggag
    15601 gaacttcaga tagtttatct atatgtgata agataaatga actgaaaaat caaccatata
    15661 tcctttcagt tactacagat tatggaaggg aacttgcaca acaacatgca gagaatgtaa
    15721 ttttaggtaa gcttggaaaa gaagatatgc taaaatttat aaaagataat aatatagtaa
    15781 aaataataga tgccactcat ccttatgcag ttgaagtatc caaaactgct attacttgtg
    15841 caaaactatt gaatatagat tatataaggt ttgagagaaa atctttaatt gaagaaatag
    15901 attatgataa taaatttata gtagatacaa ttgaagaagc ctgtgaaata gcaaataaaa
    15961 tagggacaaa tatattcata ggaacaggaa gtaaaaattt gaacagattt atagaggaaa
    16021 taagtgataa aaatttagtt gttagagttc ttccaacgag tgaagtcata ttaagttgtg
    16081 aaaaattagg tttaagtgca gataatatag tagctatgaa aggtccattt agccagtata
    16141 taaatgaaga aacatataaa cattacaata tagatttagt cataacaaaa gaaagtggaa
    16201 tagctggtgg gttcttagag aaggtaaatg catgtaaaac tttcaatata ccagtaataa
    16261 ttataaaaag agaatcgatt aactacgaaa atgtatttaa tgacataaat aaaattaaag
    16321 acatacttgt gtaattttta ggaggagaat atatgaagaa agccttatta gtagtgagtt
    16381 ttggtacaag ctatcatgaa acgagagaaa agacaataga tatatgtgaa gataaaataa
    16441 aaaattcgtt aaaaagccat gattttttta gagcttatac atcaaatatg ataataaata
    16501 aaataaaaaa aagggatgga atagaaatag ataatccaat acaggcttta gataaaatat
    16561 atgaacaagg atatgatgaa gtaataatcc aaacacttca tattatatgt ggcgaagaat
    16621 ttaacaaatt aaaagaacaa gtagatggat attcttctaa attcaaaaag attgtattag
    16681 gaagaccttt gcttacacat attgaagatt atcaggaagc tgtagaggca attaagcatc
    16741 aaattcctca tatggaaagt aatgaagcag tagtatttat gggacatgga acacttcatg
    16801 aatcacattc agcgtatcca gcattggaat acatgcttag ggataatgga ataaatgctt
    16861 atgttggtac agttgaaggg tatccagaaa tagaacatgt aataagaaga ttaaaagaag
    16921 gaaatataaa aactgtaaat ttgatgccat ttatgttagt tgcaggagac catgctataa
    16981 atgatatggc aggagatgaa gaagattctt ggaaaacaat attagaagaa aatggattta
    17041 acgtaagagt tcatttaaaa ggactaggag aaaattctta tatacaagat aaatttgttc
    17101 gtcatgctgt aaaatgtgca gagaatgcga agttctatgg tataggagca gggccaggag
    17161 atggttcttt acttacgata aaagcggtta atactttaaa gaaactggat attctataca
    17221 ctccagaatc aaaaaagggt ggagatagtc tagctttatc aatagttagt gaatatttac
    17281 ctgaatcagt ggaaataaag tctaggcatt tcccaatgag ttttgatggg aatgaaaaaa
    17341 gtttagcttg ggacaatgtg gcacaagaaa ttatatcaga tgttaatgat ggtaaaaatg
    17401 taggatttgt tacattagga gaccctatga tttatagtac atatgtatat ataatgaaaa
    17461 gaatattagg agaggtagaa gtcgaaacta taccaggaat ttcatcattt tccaatatag
    17521 catctaatca gaattttcca ttagtcatgg ataaagaagc tttagtggta attccttgta
    17581 ctatggaaga tgataagata gagtatgctt tgcaaaatta taattcaata gtattgatga
    17641 aagtatataa aaattttaaa gaaataataa aaaaattaga aaagtatgat ttaatagaac
    17701 atgctatttt agtaagtaat tcttcccaag aaagcgaaaa tgtgtttaca gatttaaaag
    17761 aagcgcacct tgaagataag atatcttatt tttctactat actagtaaat aaagataata
    17821 aaataaaata ggtgaaagca atatttaaag gagacttaat atgaatatag tggttggaac
    17881 taggggtagt aatctagcgc ttatacaaac agaatgggta ataaatgaat taaaaaagaa
    17941 gtatccagaa attagttttg agattaaaat cataaagaca aaaggagatt taattcaaaa
    18001 tgtttcactg gataaaatag gtgataaagg attgtttgtc aaagaaatag agcaacaatt
    18061 attagatgga aaaatagaca tagcagtaca tagtatgaaa gatatgcctt cttatttagc
    18121 agatggacta aaatttgctc atacgcctaa gagagaagac ccaagagatg ttctaatact
    18181 tagagaagga tataaaaatt tagatgattt gcctcatgga gcagtaatag gaacaggaag
    18241 taaaagaagg aagtttcaat tattgaaaca aagacctgat ttaaatatag tacaagttag
    18301 aggtaatgtg gaaactagga tacgaaaaat aaaagatgaa aacatgcatg gtattgtgtt
    18361 ggcagcatca ggaataatta gagctaattt acaagataaa ataagttctt atttacctgt
    18421 tgatgttgtt attccagcac ctgcccaagg agctttagct atagaaatta gaagcaatga
    18481 tagtacgata gagggaatag taaattcact gaaggatgag aatacagaaa tccaaatatt
    18541 ggctgaaaga ggttttttag atggtgtaaa tggaagttgt catattccaa tggcagctta
    18601 ttgtgaaata atccaagata aaatacatct tactggctta tatggagatt cagaaggaaa
    18661 aaaagtagta atcaagtcta tagatggaga tatttcttct cctagagagt taggtttaaa
    18721 gttagctaaa cttgtattga aggagtatga gaattatgaa gggtaaggtt tacttagtag
    18781 gggcaggacc aggtgattat aaactcatga ctttaaaagg tttagagtgt ataagaaaat
    18841 cggatgtaat agtatatgat agattagcaa atagtagtta tttaagagaa gctaagcctg
    18901 attgtgaact tatatatgta ggcaaggcat caagtaatca tattttgcca caagaagata
    18961 taaatagaat tatagcagaa aaagctaaag aaggaaaaat tgtaactcgt cttaaaggag
    19021 gagaccctta tgtatttggc agagggggag aagaagcaga gactttagta gatgaaggta
    19081 tagaatttga agtagttcca ggtataactt ctgctatagg aggtttatgt tatgcgggta
    19141 ttcctataac acatagagac catgcttcgt catttcatgt aataacaggt catttgaagg
    19201 gtgatgatag tggagaatta aactggaatg ctcttgcaaa taacaaaggt actcttgtgt
    19261 ttttgatggg aatttcaaac ctagagaaaa taagtgaaaa cttaatgaaa gaaggaaaag
    19321 ataaggatac acctgttgct cttataagtt gggcaactag atataatcaa agagttgtta
    19381 catctacact tgaaaatgtt tatgaaactg ctattaaaga agaagtaaaa ccgccaactc
    19441 ttatagtagt tgggagtgtt gtaggactta gagaaaagtt gaacttcttt gaaagtaagc
    19501 ctttatttgg aaaaagtatt gctgtaacta ggtctagaaa tcaaaatagt gtattggtag
    19561 aaaaaataat ggatttaggt ggaaatccta tagaaatacc tactattaag gtagaaaaaa
    19621 tacaaaataa tattaactta gaaaatgaaa taaaaaatat aaataagtat aattatctaa
    19681 tactaactag taaaaatgcc gtagaaatct tctttgaaaa gatatatgaa atgaattttg
    19741 atttaaggat attatctaat ttaaaggtat gtgctatagg ttctgctaca tcaaatgagt
    19801 tgaaaaaaag aggaattata gcggatatag taccaaaaaa atttgtagca gagtcactgt
    19861 atgaagagtt agcgcctatt ttaaatgatg acgataatat acttatacct agagcagaaa
    19921 atgctagaga ttatctggtt gacaaactca aggctatttg tgaagtaaca gaagtacata
    19981 tatatagaac agtaatagat gaggagaaaa aaggtgagat attagatatt ttaaattcta
    20041 atgacataga ttatataaca tttactagtt cctcaacagt aaataatctt gttgaaatta
    20101 taggaaaaga aaatattaaa atattagaaa aaataaaaac actatctata ggtcctataa
    20161 ctacagagac aataaatagt ttgggaataa aattgtacaa agaatctgct acatctacta
    20221 tagattcgtt agtagattta attataaaaa attaattttg gaggcttgaa tatgttaaga
    20281 agaccaagaa gactaagagc aagtaaagca atcagaaatt tggtaagaga aactaaggtg
    20341 aatgtagaag acttaatata tccactgttt gtagtagagg gggaaaatat aaaagaagaa
    20401 attaaatctt taccagatgt ctatcattta tcaatagata tgctagaaga cgaaataaaa
    20461 gaaataagtg agttgggaat tgaacatata atgttatttg gtgttccaga tgaaaaagat
    20521 gagtgtgctt cacaagcttt taatgataat ggaatagttc aaaaggcaat tagaaaggta
    20581 aaagaaataa attcagatat aaatgtaata acagatgtct gtatgtgcca atatacaagt
    20641 catggacatt gtgggatttt aactgatact ggttatgttg ataatgataa atcattggag
    20701 tatttggcta aaatatctgt aagccatgct aaagcaggag ctgatatggt tgctccatct
    20761 gatatgatgg atggaagaat acaagcaata agagaagctt tagatgaaaa tggattagaa
    20821 aatgttggaa tcatggctta tagtgttaag tatgcttcaa attattatgg accttttaga
    20881 gaggcagctc attcagcacc atcttttgga gatagaaaaa catatcaaat ggacccagca
    20941 aatagcaatg aagctataat agagactgaa cttgatatat tggaaggtgc agatatactt
    21001 atggttaaac ctgcattatc atatttagat attataagaa gagttaaaga taattttgac
    21061 ttacctctag cagcttataa tgtaagtgga gaatactcta tgcttaagtt tgctgtgaag
    21121 gaaggtttat taaatgaaga tgtaattctt gaatcaatta catctataaa aagagcagga
    21181 gcagatataa taataactta ttttgctaaa gacattgcta aactgttgaa aaaataaatt
    21241 ctagcagtat aaagaatatc aaaaagtata ggaattgtaa ttaactatta tacagtagtt
    21301 ttgtttagac atttattgaa gaataataga aactaatttg caatttctat actttattta
    21361 tgtaactaaa taaaatttgg aggtagaaga tgctatatcc tattaattta aaattagatg
    21421 agttagatgt agttataatt ggtggtggag aagttgcata taggaaatgt aaaaattttt
    21481 tagagtttaa caaaaatgtt actatagttt ctaaacaaat tttaaataaa ttttatgatt
    21541 taaaaggaaa tataaaaata ataaaagatg attataaaga aatatatata aaagatgctt
    21601 ctgtaataat tgctgccaca aataatagag agttaaatat ggaaatagga ttatattgta
    21661 aagcaaaaaa taagctagtg aatgtagttg ataatataga aatatcgaac tttactgttc
    21721 catcatatat aaaaagagga gatttactta taagtgtgtc tactgggggc aagagtcctt
    21781 cattatcatc aaagataaaa aaagaaattg aagagagata tactgaagat tatgaggagt
    21841 atttaaatgt attaggagat ataagaaaaa aggttataaa aaaatacgaa gataaaagta
    21901 aaagaaaaga tgtgcttaat atgttggtaa cacttgacct agaagaactt aagaagtttg
    21961 atatataaaa aataaaataa aaaatgttaa aaaggtgttg acggattatt tttaagatga
    22021 tatagtatta cttgtcctta agaaaagggc aaacataa
//
LOCUS       DAYASV010000059        21455 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYASV010000059 DAYASV010000000
VERSION     DAYASV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.059"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            113..985
                     /locus_tag="ACGI68_002132"
     CDS             113..985
                     /locus_tag="ACGI68_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFH0138567.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(1182..2369)
                     /locus_tag="ACGI68_002133"
     CDS             complement(1182..2369)
                     /locus_tag="ACGI68_002133"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0138568.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(2374..3444)
                     /locus_tag="ACGI68_002134"
     CDS             complement(2374..3444)
                     /locus_tag="ACGI68_002134"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0138569.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIKLIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            complement(3454..4776)
                     /locus_tag="ACGI68_002135"
     CDS             complement(3454..4776)
                     /locus_tag="ACGI68_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0138570.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILSNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEGLLDDLGLDF"
     gene            complement(4808..5143)
                     /locus_tag="ACGI68_002136"
     CDS             complement(4808..5143)
                     /locus_tag="ACGI68_002136"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138571.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            complement(5240..5545)
                     /locus_tag="ACGI68_002137"
     CDS             complement(5240..5545)
                     /locus_tag="ACGI68_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFH0138572.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            complement(5560..6603)
                     /gene="ypdE"
                     /locus_tag="ACGI68_002138"
     CDS             complement(5560..6603)
                     /gene="ypdE"
                     /locus_tag="ACGI68_002138"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0138573.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNKKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            complement(6606..8507)
                     /locus_tag="ACGI68_002139"
     CDS             complement(6606..8507)
                     /locus_tag="ACGI68_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138574.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVVGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            complement(8833..9684)
                     /locus_tag="ACGI68_002140"
     CDS             complement(8833..9684)
                     /locus_tag="ACGI68_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFH0138575.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVNIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(9733..10896)
                     /locus_tag="ACGI68_002141"
     CDS             complement(9733..10896)
                     /locus_tag="ACGI68_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0138576.1"
                     /translation="MFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKLSR
                     FIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNPYD
                     YLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKNDEN
                     NYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVIDRK
                     DEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPKSF
                     IDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFKEK
                     YNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            11373..12290
                     /locus_tag="ACGI68_002142"
     CDS             11373..12290
                     /locus_tag="ACGI68_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0138577.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAINGEECIKAQRLLEKIINSVK"
     gene            complement(12419..13348)
                     /gene="pfkB"
                     /locus_tag="ACGI68_002143"
     CDS             complement(12419..13348)
                     /gene="pfkB"
                     /locus_tag="ACGI68_002143"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFH0138578.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGMNIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            13617..14342
                     /locus_tag="ACGI68_002144"
     CDS             13617..14342
                     /locus_tag="ACGI68_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0138579.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            14425..15579
                     /gene="nagA"
                     /locus_tag="ACGI68_002145"
     CDS             14425..15579
                     /gene="nagA"
                     /locus_tag="ACGI68_002145"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0138580.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDNDFNIHETYINGICKYKVNK"
     gene            complement(16288..16758)
                     /locus_tag="ACGI68_002146"
     CDS             complement(16288..16758)
                     /locus_tag="ACGI68_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFH0138581.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIY"
     gene            complement(17362..18519)
                     /locus_tag="ACGI68_002147"
     CDS             complement(17362..18519)
                     /locus_tag="ACGI68_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFH0138582.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            18673..19242
                     /locus_tag="ACGI68_002148"
     CDS             18673..19242
                     /locus_tag="ACGI68_002148"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFH0138583.1"
                     /translation="MKKEFRKKVIESRKNKSANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNN"
     gene            complement(19424..19840)
                     /locus_tag="ACGI68_002149"
     CDS             complement(19424..19840)
                     /locus_tag="ACGI68_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138584.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(20066..21160)
                     /locus_tag="ACGI68_002150"
     CDS             complement(20066..21160)
                     /locus_tag="ACGI68_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138585.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
BASE COUNT         7325 a         3472 c         2462 g         8196 t
ORIGIN      
        1 attttcaaaa tatactaaaa tctacctttc aaatataata tcaaataaaa tttaataatc
       61 tttatacttt atagtataat atactcataa aatataataa aggagatttt aaatgaatat
      121 attacaaaat gaaaatttaa ttatcgaatc aagcagtttt ggtgctgaac taactaaaat
      181 attttctaaa aaatacgaca aaaacattct ttgggatggt aataaaaaat attggggtag
      241 acaatcacct atattgttcc ctatagtagg taaacttttt gataatgaaa caataataga
      301 agaaaattta tatagcatga ctcaacatgg gtttgctaga gatatggact ttgagattgt
      361 agataaagga gatacatttg taacttataa attaacagat aatgaatcta ctttaaagaa
      421 gtatccatat tcttttgagc taatcataaa ctatacatta aatgataata gtattgaggt
      481 agaatggatt gtaaaaaata ctgattctaa agatatattc ttttctatag gtggacatcc
      541 tgcattcaac cttccttttt acgaacaaaa tgatttttct aaacactacc ttgagtttaa
      601 aagtaaaaat gatgtagaaa aaatcaattt aaatggttct tttacagatg atataatacc
      661 tattggaaaa ttaaaaaact tacagttaaa tccagaagta ttcaagaatg atgctttgat
      721 atacaccaac atagatgaag tgtctatatg taataatgat ggtagtaaat atgtaactat
      781 atctatggaa gatttccctc tagttggtat ttggactcct tattatagtg aaactaattc
      841 tactgctcct tttttatgta tagaaccttg gtatggtctt gctgatagta taaattctaa
      901 caaaatttat aaagataaga agtttattaa taaacttagt aaaggaaaag tttttactgc
      961 ttcttacaat ataaatatac actagataaa ggttagctaa cccgttccca aaataataaa
     1021 atatttttat ataagttaat ataagggtat gaaataatat tatttaatat tcctcaataa
     1081 tattccttaa tcaaaaaata ttactggaat acaaaattcc agtaatattt gataactaaa
     1141 taatgtgttt ttatataaac catagatact ttttttatga tttaacaata catacttata
     1201 tacgtttttt tgattctatt tagagcctca tttaatgttt ctctaggaca ccccacatta
     1261 atacgaataa atccatctcc atctttcaca aaataacttc caactaccat agttaagttt
     1321 gcatgtctaa aaaattcttt agattcttct tcatttctaa atacttctct tacatctatc
     1381 caggctaaaa aagaagagtc tgacttcatc actttagctt ttggtatatt agtttcaaag
     1441 aaatcataaa ctatttcaaa attctcctct acataactag tcatagcatc tagccattct
     1501 tcgctttcat tatatgctgc aactattgct ggtattgcaa atacatttgg agaagttatt
     1561 gaattacttt ttagcctttc taataaagtc tccctaattt ttacatctct tattaataca
     1621 tatgatgttt ttaatccacc taagttgaag cctttattag gagatacaca gattatacta
     1681 tttttataaa tttctggatg agcattccac aaagatacaa atctataatc tttaaatatt
     1741 atatctctat gaatttcatc acttaccaaa attacattat actttaaaca taactctgac
     1801 acttgatata gttcttcttt tgtccatata cgacctgatg gattttgtgg actacagaat
     1861 ataagcaatc ttatattctc gtatttcatt tgattttcca tatcctctaa atctaaataa
     1921 tatctattgt tatctaattt taatgagcta caacacaact ttctattatt atttactaca
     1981 gcctctgcaa aagggtcata agcaggtgta tttatcaata caccctctcc ttctttagta
     2041 tatgcttgga ctatataatg aagggttgaa acagtaccaa aagttaattt tatccaatcc
     2101 ttttctatat atacatcaaa tcttctttta ttccaattta taatagcatc atagaagtca
     2161 tctcctataa aagtataact ataatctcct actgatgctc tttttaagat agattctttt
     2221 atagcaggag cacactcaaa atcgatatca gctaaatcca taggaattgc atcttcattt
     2281 aagccaaatt tttctttaat cacatggttg ctccattttc tacaatttcc ttctttacga
     2341 tttaatactt tattaaaatt atatttcata attttactct cctaatatta taaactcttt
     2401 ggatgtctta ttgagtggaa caccttttcc atctattata acaatatcat cttctattct
     2461 aactccccct acatttggaa tataaatacc tggctcacaa gacattacca ttccttccac
     2521 taatatatct tcactatttg gatttaaagt gggaacttca ccatctccaa gacctatacc
     2581 atgacccagt ccatgattaa aatattcgcc aaatccatgt ttatctataa tttttctagc
     2641 cactttgtca acatcacttg ccctagttcc ttcctttatt gactctattg cagataattg
     2701 tgcttccaat actacatcat atatctcttt aataatttta ggaggtttgc ctatagatat
     2761 tgtacgagtc atgtctgatt gatacccttg ataaacaaca ccaaaatcta ttgttatagc
     2821 ttcattcact ttcaattttt tgtttgtagg tcttccatgt ggcattgcac ctctttctcc
     2881 tgataccact attgtttcaa aagacattcc acttgcacct ttttttaatg catgatactg
     2941 aattagagct gatacttcta gctcagtcat atctttttta atttcagaaa taacctcatc
     3001 aaatacttca tctgtaatct cacaagcctt tttaataagt ttaatttctt ctttagattt
     3061 tatagccctt agcttccata attcatttgt tattaaattg atttcaaaac cttcattttt
     3121 tagtgaaaga tactcttgta tactcatagc ttggttttct attgctaact tttttatatt
     3181 taattctttt aataactcaa ttatagttgg tatataagaa ccttgagata caatcctttt
     3241 tatgaaacca gtcgtttttt tatctgcttc atcaatatat ctgccatcta gaatttgata
     3301 atttttatcc ttagtaacaa gcacatatac accactacct gataaagctc ctatatatct
     3361 tttagtattt tttgctttaa tcaaaagagc gtcaacacct cttttataga ttaaggatac
     3421 taatctctcc attgtattat tcataacaaa atattagaaa tccaatccta aatcatccaa
     3481 taagccttct tcttctttag agaatatatt cttagtttct tctttttgtt ttttattatt
     3541 ttgttgacgt tccattattt taaagaatgg ccaatataat aaaactgcta atactaattg
     3601 caatgcattt gctaatatag aatgccaatc tccattagtt aaaaatcctt ctaagaatac
     3661 ccctacatat ggagggtcaa atactggctt tgctaaccat ccccagtgca taaaaaacat
     3721 tggtagtgta cctattatag ctccaccaaa tacatgtggt ataaacatta ttggatttaa
     3781 tattatcggt gctccaaata atattggttc atttatccca aataatgcag ggaataatga
     3841 tacacgtcca atcttcttat aggaagcatt ttttgataac atacatgcaa ttacacaacc
     3901 tatactaatt ccacatcctg taaatccaaa gaatgcactt gaagctcctg ctgtaaagaa
     3961 atgaggtatt gtttctccag cctgcatagc tgctatgttt tcagctataa atgtaatcat
     4021 tataggtcta gttataggac taaatactga tgaatgtatt ccaaaaaaga atatagtaca
     4081 tatagcaaag ttaagtgcaa gaactgacca tgggttatcc atacttccta ctaatggaga
     4141 taaaaattga gtaagtatct gtggaggtaa ttctccaaat acttttaatg aaataaaacg
     4201 agcaactata aaagttccac aaactattat agtagtaggt attaattcaa aagacctaga
     4261 tacaaaatca ggtacatttt ctggcatttt tattgtgata ttcttcttat tacaaaaatt
     4321 atataactcc acagaaccaa gccctgctat catacctgta aatagccctt tagctcctaa
     4381 atttcctatt tctaaagctc catctggtgt aaatgaggct actaggaata aaaaagaaaa
     4441 agttgatagt gcaacacaac ctggtatata taatttatag tagttcccca gattaaaagc
     4501 aatacacata caagcatata atcctattaa acctatggaa agacttactg gtaaatccaa
     4561 tattgctgaa ttgctaagta tgaatttgct tattggatta ttttctccaa gcatatttgg
     4621 aagtgctgga agtattgcaa atatacttcc taatattgtg aatggtgtca tagcaaccat
     4681 accagccttt atagaaccaa ggtgacgttg attgtcaacc ttatgagcta aaggcataaa
     4741 tactctattc ataaatgctt caaattttga aaacatatct aatctccctt ttccctcatt
     4801 atttttatta tccttgagct aattgatgtt tcttataagt tattaatgcc aacttcataa
     4861 ctgtagctcc atccattttt ccatatacat ctttatctat tactgcaaaa ggcttatttt
     4921 ttggctctaa tattcctttt aagaagtcta ttttgtgagc tatttgagga cctataagta
     4981 ctatatccca atcatcaact tcactttcta ttgaatctgc tggtatagct gtaaactcaa
     5041 aatctttttc attgagttta gcactctctt ttaccacctt tttcatattt tgcattaaca
     5101 tattagtaga aaatccacct gcacaacata ataatatttt cataataatt cctcctaaag
     5161 tttgtatttt tattatctct taaaagcatc taaactttta aaagaatttt tacataaata
     5221 atctatttct atattacaat tacttatcaa gacaatatat caaagcctct atcaagtctt
     5281 ttgctagctg agaagtcata tagtggtctt gagcatgtac cattaaaaga ctcattgctg
     5341 gactttcaac atcttcattt aaatcttgag ttattaattc tgtttgtatc ctgtgagctt
     5401 caatatctat tttacgagcc tcctctatta acaattttgc tcctttaaaa tcttttgact
     5461 tcactttttt taaagcctca aaagctttcg atttactctc tcctgcagtt gatataagtt
     5521 ccattatttg tacttctgtt gacattttct tattctcctt tagataaaat ttcttatatg
     5581 tttatattgt tcttcatcaa aatttaaaat taactgtgta tacaatctta taatgttctc
     5641 caaatcttta ttacttacta ttgaaaatgc acaatgacca tatctaactg gtacacaagt
     5701 tactacacaa ggtttccctt catttacgag atgtgcttct cctccatcag tcccacctga
     5761 ttcaaacata tcatgttgaa gtggtatatt atttttatca gcaatttcca ttacataatc
     5821 tatcattttt ctactaggga ctagagtcct atctctatgc attagcatta cacctttatc
     5881 aatttgtttc ttatttgtat agtctctcac aaactcattt ttagcacatg ctacatctat
     5941 tacaaatact atatctggat ttattaactg tgttgatgtc tttgctcccc ttattccaac
     6001 ctcttcacta cttgtagccg caaagtgaat attacaattt agattttctt tcctaagttc
     6061 ttttaaaact tctcccatca caaaacatcc tatcctatca tcaaaagcct ttcccactaa
     6121 tcggtctggt aaagtaaatt cctcaaagct tgttgtatat gttaccatat ccccaacatt
     6181 aatccctagg ttatatacat cttcttctgt atatgctcca atatctgcat atatattttc
     6241 agccttacca ttattatatg tagcatttat gacagcgggg attttcttcc cttcttttgt
     6301 tgtaattcta actttttgta caaactgtgc aagaggtttt actcctccta ttgttattaa
     6361 gtgaatcata ccaagtttgt ctatacttct caccataaat ccaacttcat ccatatgagc
     6421 acaaatcatt acgtttggtg cactctcatc ttttatttta gaaaagatga tactccctag
     6481 cccatcacag ataattttat cactataagc ttttagttct tccaataaaa catgtctaac
     6541 ctcttcttca ttagatgcta taccatctgc ttctgacaac ctcttaaaaa actctatatt
     6601 catgtctaac ctcccagata gatttttaac ttatacataa actcttcata agattgtgac
     6661 ttatttagca tgtctaccat atccttattt gccataatct tacttaaata tttagatatt
     6721 tgcttatgaa gttctaaatt tccctttatt aatgaaatca tgaaaataat tttaggcacc
     6781 ctattttcat aaataatatc agtttgtatc aaagctactg atattatatt tttgtttcct
     6841 gtcatctcga ttggatgagg tatagccacc ccatttgtat aaatagtact caaataactt
     6901 tctctttccc atacatcttt gggataggtt ggagaacaag ctccctcgtc aactatacat
     6961 tttgacatac tatcaaggat atccttatac ttagctttat cttttataca cctaaagtga
     7021 ttcttatcaa atagacctaa tatatattgt tttgggcttg atatatttcc tatatggtct
     7081 gcaaatctaa cactttcttt aatatctaaa taatccaatt cgtctaaaat ttcatttata
     7141 agtataactg gagcatttat ttcaaataaa agatttgtta ttgaaaaaat caaatctggt
     7201 gcaaattcta ttacagcttt ttcttctagt aatgaaaaat ttcttatctc agcatttgga
     7261 aaaatttctt taagtcttaa atttatcaaa aatgcactac ctccaccaga agagcatatt
     7321 attgctattt tatatttttt attaaaatta gcttcttctc tcttttcaaa aggaacagca
     7381 atgtgagtag ctataaaccc tatttcatct tgacttattt ttatatgata ctcattttct
     7441 atgatttttg aaaattgaat agataaatta aaaacagttg gatactgttg acttatttta
     7501 actgaaaaag gatttattat cttgtggttc ttctttatac gttctattaa acatgcaata
     7561 tgtaagtaaa ataagttaaa aaactcttta tcttctaaaa aattagagtt atatttttta
     7621 tctatctctc taaaaaattc gaacataata tcttgaagtt tattcttatc aatattcttt
     7681 atagtggctt tcttatcttt agtctttagt tttataagct cctttaaata atacttttca
     7741 tcataattaa ggttatattt tttatctcta aaaattttat ctaataattt atctataatt
     7801 aaatcttctg tatataattt taaatcgcta gcatttccca ctactctcat actaactctt
     7861 atatacaata taataatttg tgctaatatt tcctcaagtt ctgttaaatt tgcttctaaa
     7921 tcatgttcat taagtacatt ttttattgta cttctaatat tagctaattt taaattatct
     7981 ataaacttat tcctgtactc tgttatattt ctattccctc taaaataact atccaccaat
     8041 atactttgaa tttcttttat agaaccttct attttaattc cataatgagc ctttcgctct
     8101 agttttaatt ctgtatcctt tatcttttca tctatctttt ttaaatcatt ttttatttgg
     8161 gatgaactta ctagaaattt tttacttaac tgttctatag ttttataacc ttcatttact
     8221 aaaagctcta ctattataga ctccaaccgt tgttctggat tttcaactaa atctgagggt
     8281 aagtttctta gataatgctc gaatttatct tggtctttaa tatttagaat atatcctaat
     8341 ttagacttat attctaattt aaaaccatta cattctttta tagtttctat cttttttatt
     8401 tcatttctga tagttcgagt agataccccc aacttatctg caaaataatc tatgtttaga
     8461 taaccactac tattttttaa tgtttcaaga ataatgcgtt ctctcatatc cccacctccc
     8521 tcagtgttat tattgtgttt tttaaagtgg tataaaaaat attttttata aaaacaacct
     8581 tttcttttta tttaagaatt ttatatccta gtatatttta attccacaat ttaatataaa
     8641 tattttataa taataaaata tatagttcaa tttttagtat attttaatta tatatgtaat
     8701 actttaccaa taaaagctga tatttttcat caattaaatg aaaaaaatac accatttaca
     8761 tataattaat ataaattttt catataataa atatcctaaa cttatctaaa ataaatttag
     8821 gatattttat ttttatactc tatttacaga cccacacact ttaatctttt ctttcacaaa
     8881 atcctttact gattgaagcc ctgctaatcc atacttttta gggtctataa cctcagaatc
     8941 ttcatctaaa acttttttaa cccctttact atatgcaatt cttaattcag ttgcatagtt
     9001 aacttttgca atacctcttt ttatagcttc ttttataata tcatcatgta ttcctgaacc
     9061 tccatgcaat actaaaggta ttgaaactac atttttaatt tctgataacc tatctaaatc
     9121 tattttaggt tcacccttat acaatccatg tgcagtacct atagcaatag ccaatgaaga
     9181 tattcctgtt ctatcgacaa actcttttgc ctcttttgga tttgtataat cattactatt
     9241 cccgccatct aaatcatctt cttttccacc aactttccca agttctgctt ccactggtat
     9301 aagagaagga ctacatgctt ttactactga tttagatata gatatattat tttcaaagct
     9361 ttcatgagaa ccatctatca ttattgatgt atatccactt cttaaagctt tcattgcaag
     9421 gtcaaaactt gaaccatggt ccagatgcat tgctacaggt atatttactc tttctgcagc
     9481 aacctttgca tttgctagat aataatcaag acctgcatat tttacagtag atggagtagt
     9541 ttgtaatatt actggagaat ttaactcctc agcagcttct ataacagcaa ttaccatttc
     9601 catattttct acattaaatg ctcctactgc atacccttca tcttgtgcct ttttaagcat
     9661 tgatgttgtt gttactaatg ccacattgat accctcccaa attatttagt tataatattt
     9721 taatcttatc ttttaatatt catatattgt tacacctttt actactcgat ttactgttcc
     9781 tgtagctgat ggagtatctg gcttatttcc aacctttact gatacaaaca atgctatagt
     9841 ttgtgcaaac aatatgtatg gcattgcaag atacacatct ggtaatttat tatacttttc
     9901 tttaaactca aactttgttc ctgagaagtt attttcattt tctactgaca ctgcacaagt
     9961 gaatttagca attttatccc tttttatctc ctctaaaaca tctaaatcat attttcttgt
    10021 atataaacag ttagatacaa attcaaatac aagtgtattt tcatcaataa atgattttgg
    10081 tccgtgtcta aaccccatag gagaatcata cactgtagat atttttcctg ctgtaagttc
    10141 taaaagtttt agttgtgctt cttgtgtcaa accacctaat cctcctgaac ctaaatatac
    10201 aactctatca aaatctttat ttattaactc atgtatttca tcttttctat ctataacatt
    10261 tcttcccatt tcaattattg catttatata tgacttttct acatcatctt ctaaactatc
    10321 gaatattaac atagcagata acatcataca cgaaaaactt cctgtcatgg caaagccttc
    10381 atcatttgat tttgatggca ttaataacaa gtaattattt tcgtcgtttt tagtcatctt
    10441 agccagttct ccttctggat tacaagttat agctaaatga taaaaattat ctacaatctt
    10501 attccctaaa tttaaagctg ctaaactttc aggactattt ccacttcttg caaatgaaat
    10561 taataatgtt ggtatatctt tttttaagta atcatatggg tttgaaacta tatccgtagt
    10621 aggtattgct tcaaatatgt attttcttat gtcattttta tttttaaggt aaggaagtat
    10681 tgaatttcct atgtaagctg aagttccagc acctgtaaat ataactctaa actgcccatg
    10741 attattactt attgtatcta tgaatctact taatttttct ttgttagatt tatatatttc
    10801 ataagtttct aaccataatt caggctgttg ttttatttca ttagttgtta ttattgcacc
    10861 taaatctttt agtttttgtt cttctaattt aaacattttc attctccttt catacttgta
    10921 acgtttacat ttggtaatga ggttataacc actttaaact ataaaaaaat atactctttt
    10981 ataatttttt gcttagattt tttagtacta cttaaactca ctataaaata tacactatag
    11041 attttaatta attttatagt ttatctttaa aaatttaata aaatcatact ctatatactt
    11101 tagtattttt attttacgat aaaagcttgt tcagaccaaa gtggtaatta ccactaatcc
    11161 aaatataaat taatttgaat tttttgtcaa tacataaaat atttatatca atcaatttta
    11221 tggatatcct acaaaattac tttatcaata tttcttattt cgctaattta cttatataag
    11281 ctttaaattt attttttgat acaaagttta caaatttatg tgtctgaatt ataatcaatt
    11341 ctgtaaatat aaaaacctta ggagaaagta ttatgaaaaa tataaaaatg ggaatgatag
    11401 gtcttggctc catagctcaa aaggcatatt tacctatatt aactaagtct gagagatttg
    11461 agtttgtagg agcttttact ccaaataaag ttaaaaggga aaaagtctgt agtgattata
    11521 gaatcatgcc attcgattct attgaatccc tagctaaaaa atgtgattgt atttttctac
    11581 atagctctac agaaacacac tacgaaatta taaaaatatt attaaattta ggtgttcatg
    11641 tatatgtaga caaacctctt gcttcaacag taagtcaagg agaagaactt atcgagttaa
    11701 gtacgaaaaa aaacttaaat ttaatggttg gattcaaccg tagattctgt cctatgtaca
    11761 aagaaattaa aaataatgct acagaaattg tatctatcaa tatatgtaaa catggattaa
    11821 attccttaag aaatgttaga ttcgacagta ctcttataga tgactatata catgttattg
    11881 acactgcttt atggttagca aatgaagatg ttgaaattag tggagaggat ttatttttaa
    11941 cagacaataa gaatcttata tttgtaagtc ataaacttaa aggtaaaaat ttctctataa
    12001 acacctctat gcatagagat tctggaacaa agttagaaca agttgaaatt ctttcaaagg
    12061 gtaagattca aagagttaag aatcttaatg tactggaaat agaagaaggt ggaaacctaa
    12121 ctttaaagca aagtggtgca tgggtaaata tattaaaaca aaagggtttt gaggatatat
    12181 caaatcattt tattgattgt atagaaaata atataaaacc agctattaat ggagaagagt
    12241 gtatcaaagc tcaaagatta ctagagaaaa ttataaattc agtaaaatag atatttatta
    12301 taaaaatagg ttaaaaatga atttttctaa cctattttta tataagacta tatcttttat
    12361 attaaaatta aaatctatta caacaacatt aaatttatta tattcccatt aaatgtattt
    12421 aacttaactt ttttacttca actaaatctt gatatttatc aatttctccc aaatctacaa
    12481 atccagtttc catatttaaa gcattactta taccacaagc atttgcaaat ttaagtaaat
    12541 tttctatttt ataacccctt aaaataccta cactaaatcc agccactgtg ctatctccac
    12601 tacctacagt atttatacac tttacaactg gtacttttac atcatataca ttatcttcgt
    12661 ttaaatacac cataccatct tttcctaaag atatacatac attctctgca cccatttcaa
    12721 taagtttttt tcctgacaag ataatttcct ctcttgattc tatattcatt cctaataaat
    12781 gtcttatttc atctatattt ggttttatta aatatggttt tgattttaaa gctattttta
    12841 atgcttctcc tgatgtatct aatataaact ttatattatt tgctctacat atatttccta
    12901 tcttttcata atagtctatc ggcatattct cacaataact accagaagct accagtattt
    12961 tagtattttt tagtacttcc tctatatcat tttcaaatct ctctaaatcg cttttttcta
    13021 ctattggtcc tttttctaaa aattctgtac taactttatt gtcctcaatt atgtttaagc
    13081 aatttctagt ttcctgctct atttttgtaa atttattttg tatatctagt tttctaagtt
    13141 cattttttat aaattctcca ttaaatccgc ctagaaatcc catagctaaa ggattttctt
    13201 ttaaaatttt acatacctta gctacgttta atcctttacc tccagcagtt gcattcttac
    13261 ttactaccct ttgcacctca cctatgttta tattattcac tctatacatt ctatctatag
    13321 atgggttgaa tgttattact gtaatcatac tagattctcc tcattattaa tttttacttt
    13381 tctaaacaat aaattaatct gtaaaataaa aaatacctca tctataatat acccaaagtg
    13441 gttattacca atttgaatat aaattatttt acttgttttg tcaatgtacc ataattttat
    13501 aataaattaa taaccttact tattgaattt tgattaaact ttattcattg acataaataa
    13561 ttctatattc tataatcaaa ttgtggtaat aaccacttag aaaggactat atttttatga
    13621 taaaaagaaa tgataaaaga ccaatatatg accagcttgt tgagatatta agaactaaga
    13681 ttgaaaatga gatggaacca aacgatagaa tgctttccga aagaaaaatt tgtgatgaat
    13741 atggagtaag tagaaccaca gttagactag ctatggctga gttggaacat atggggtata
    13801 tatacaaaag acatggaaaa ggaacttttg ttgctgcact aagtcaaaat tctcaaaact
    13861 taatggaaag ttatagtttt acagattata tgagaacaca aggaaaaaag ccaggtacaa
    13921 aggtattatc atttgaggta gtagaaagta caaagtattt ctctgaaaaa ttaggtataa
    13981 ctcctggtga aaaaatgata aaaataatgc gtttaagact tgctgatgac cttcctatga
    14041 tgcttgaaag aacatatctt cctatgaaag aatttatagg attgacaaaa gaaaaaatcg
    14101 aacaaaaacc tttatatgaa atatttagag aagattatgg cgagattatt aaagttgctg
    14161 atgaagaatt ttcagctgga ataatctctg ataaggaagc taagttattg gaagtaccag
    14221 gagattcacc ttgtctgaaa ttacttcgta ccacatataa tgatgacaat atagttattg
    14281 aatttacttt gagtgttgct agaagcgata aatttgtata tagagtaaga catgtaagat
    14341 agtaagttaa ataactttca agaaaataga atgatattat aaacgtcaat gccacaatga
    14401 tagtataatt ataaggaggt tattttgaaa agttttattt atgcagataa attttttatg
    14461 aaacatggaa tcaaagaaaa aggttacctt agtataatta atggcaaatt cggtgaattt
    14521 caacaagaaa gacctaatgt tactgataat gttatagatt tttcaggtaa atatattgct
    14581 cctggcttag ttgatactca cattcacggt cttttaggtg cagatgtcat ggacaataca
    14641 tttgaagcaa taaacacaat ttcaaaaggc ttattaaaat atggagtaac atctttccta
    14701 cctacaactt taactgattc tgtagataca ctaagtgatt ctgtagaaaa tataagcaat
    14761 agcttcaatc gagttgaggg tgcaaaaata caaggtattt atttagaagg tccatttttt
    14821 acagaaaaat ataagggcgc tcaaaatgaa aaatatttta aagaccctga catcaatata
    14881 ttaaaattat ggcaaaaaaa atcgaaaaat ttaataagaa aaatagcaat tgctccagaa
    14941 cgtaaaggtt caatagaatt aacaagatat gcaactaaaa ataatatatc agtttctctt
    15001 ggtcatagtt cagctacatt tgagcaagtt aaacaagtcg ttaattcagg tgctaaagtg
    15061 tttgtacata catataatgg gatgagtcct ttaaatcata gagaacctgg aatggttggg
    15121 gctgctatgg tactaaaaga tacttatgct gaattaattt gtgatggtca tcatgtaagc
    15181 ccaatagctg ttaaaataat gatggatgcg aagtctagta ataacatagc tttaattaca
    15241 gattgtatga gagctggtgc tatggaggat ggaaaatata ctcttggtaa gtttaatgta
    15301 aatgtaaaaa atagtgttgc aagattaaat agtgggagtt tagctggaag tgtattaact
    15361 atggacaagg ctgtgagaaa tatagttgat tggaacatat caactttaga agatgcaata
    15421 aaaatggcta catatgtccc tgctttaagt tgtaatattg ataatgtctg tggctctata
    15481 tatcaaggta tggatgcaga ctttatagta acagataatg actttaatat acacgaaact
    15541 tatataaatg gtatctgtaa gtacaaagtt aataaatagg ttcaatataa ggttatggag
    15601 gctgtctaaa aagagtctgg aaatatttaa agttatttct ccaaaatctg atttaacact
    15661 tttatttcca tatcgttaat aaactattgc gatagaggtt attttaaaca aatatatatt
    15721 ataagtattt ttgtgataat attttcgata atatgtacat ttaaagtaac ttttctatta
    15781 ttatttgtaa gtttttatct tgagcaaaca tataaatttt taataaagca tgagataaac
    15841 cctatatttc aattactaat gatgagattt tgattgataa cagatatatt ttcaaatgta
    15901 aaaatactaa aaatcatatc aattttaaag atattgtagg tgtaaataaa caagataata
    15961 ctatagaaat ttttacaaat gaaagcactt attgtatata tctaaaagtt ttaagcattg
    16021 agagcgaaga atatctaata gatagactaa aatcattttg ttgattttat atattttatg
    16081 ttaaatataa agcagagtag ttaaaactac cctgcttttg taattaaaat ttaaaaataa
    16141 taactcataa tacattgaat ttgagcttaa cagtaactat tgtaataata gtatcaatct
    16201 tttgtgttga atataattta tttacactat agttaagcat gatttctaat gttattttct
    16261 ctaatttaca attatgatta aaattcatta atatatatat ttataagttg tattgtttat
    16321 attgtttatt acttttttaa atccactagc tgtaataata aatgtctttt ctggtatccc
    16381 tggtgaaaag attattttat catggttaaa tatatcttga tctattacaa gagagatatt
    16441 tttctcatat ccaaaaggag atacacatcc aggcaaacat tgtgtttttt gtgttaattc
    16501 ttcttttgaa catatagata cttttccacc taaaacattt tttatagttt ttggatctaa
    16561 tttttttcct tctgtggtta caaatatata gtaatttcca gatctatctt ttataaataa
    16621 acttttactt tctactccag tatattgtaa tttttctctt atttctttag ctttttcaaa
    16681 tgataaaata ggttcatggt catattcttc aaacgttata tgattttctt ttaaaatatt
    16741 tttgatattg ttgtacatat attttccttt ctatatttaa attaatttat atataatatt
    16801 caattataaa gtgattttat aatttcttca aactatcata aaaaacattc acatgtttta
    16861 ggaataaatt atataaaata ggttataaga taatgaacat ttatattata acctattttt
    16921 atatcgataa gtatgaaatt gtttaaccaa gtcaaaatga tatataaatt tatatttaaa
    16981 tgtgtttaga gtttaattta ttttcaatgt ttaaaaaagt tattctatgc atctaattta
    17041 attagtaaac tgaggttcta gttagataaa ttatattata atgaatatat agtatgcagt
    17101 ttacgatatt ttaaaagtaa atgatataat atttggaaca actgggttgc aatgtgtttt
    17161 taaaaaatat caaaataagg ctatgatttt taaaaccata gccctgtctg taaaaattta
    17221 attatcactt aaaaaataaa tttttattta aagaatacac ataaatattt ccagactccc
    17281 tcatgaacta aaaagttcat tttgagacat ctcttttata atgttcttta ttttatagta
    17341 taagtttata aaaattaaat cctatttatc aatctgttct ctaagccact tagttgctac
    17401 atctaactgt ttattttcat ctttttcttc aatagtaggc tttatacctt tttcattgat
    17461 ataatctcca ttaggtgtaa aatattgagc agtagttaac ttgtatcctg ttccatcctt
    17521 caatcttaca actgattgta ccaacccttt accaaaagtt gtagtcccta cagaaatacc
    17581 ttctttatta tcaacgattg cagctgttaa tatttcagca gcagaagcac tttctccatt
    17641 agttaacact actattggaa gtcccaattt ttctttatca gatttcaagt actcagtatt
    17701 tcccttatta tcttttgtat atactatagt tccttcacca attaactcat cagcaacttc
    17761 tttacatata tctagcattc caccaggatt atttctaagg tctattacta atcccttgat
    17821 attttttgct tttaattctt ttaaagcttt atcaaagtca gtatatgtac ttgatataaa
    17881 ttgggtaatt tctatatatc ctaaatcatt atcaattact ttactttcta tactcttctc
    17941 aattatctgg tctgttttta ctttaaagtt caattgttta tcttctctta atatagtcag
    18001 ttcaactgtt ttaccttttt tacccttcat catacgaaca gcttcatctg aatttttata
    18061 tgagacagat tttccattga cttttactaa tttatcccct gattttacac ctgctttctc
    18121 tgctggagaa tctttcatgg gaactactac taagccacca tcttttgatg cccctatata
    18181 catacctaca ccaacataag aaccttcact atcattaatt agattctcca tttcatcttt
    18241 agtataataa ccagaatact tatcatttgt tcctagaaat agaccttttg ctgctccatt
    18301 taccaaatca gtatctgata cttttttata aaaatcttgt tttataatag attctagtcc
    18361 taacaactta tcatattttt tatagtcttc atagagttct ttcgatatta caactttatt
    18421 ccccaatgtc aactgaaatg ctgatgtaac catagctgtt ataattacta aaatcacccc
    18481 taaaaaaata gctttcttct ttgaaatcac aagatacatc tccttttaca taaactatta
    18541 ttattatatg actagctgtt atttttatta catttaccca aatatattgc tttattacaa
    18601 taaattatac taaaatttat attaaataca attgctgtac acattataac atattaaggg
    18661 aggaattatt ttatgaaaaa agaatttaga aaaaaagtta ttgaatcaag aaaaaataag
    18721 agtgcaaact tcgtacttag taattcaaat ttaatcactg aaaaattact tcaaatggaa
    18781 gatataagaa atgcaactaa tataatgctt tatctaaatt ttaacaatga agttcaaaca
    18841 gataatttaa tcaaaaaact tttgtctcta aagaaaatag tatcaagtcc catcacaata
    18901 aaagaaaatc atacattaat ccctactcaa ataacagatt taaaagaggg attgagaatt
    18961 ggagcatatg gaataagaga acctaacgaa aaatcaccta caattgatat taaggcctta
    19021 gatgttgtaa ttgtaccagc tgtagcatat gatatacatt gctatagatt aggatatggt
    19081 ggaggatttt atgatagatt tttagaaaac ttaagaaaag atgctataac tatcggcata
    19141 gcatttgact tccagatatt cgattctatt ccaaaagaaa ctcatgatgc acaattagac
    19201 tacatagtta ctgaaactag aattatcact ccaaataatt agaatattaa gtatttccag
    19261 accccattta atatagtaat aaatactact gttaatatct tactttataa gcattttaag
    19321 tcatggcttt tacaacaata agaaccatat acaatttact tacattaata aaaaccatga
    19381 ctttattgta caatatgcag ttatttaatt ataaaatacc ttcttatttc gtcgcattta
    19441 attctattct atctggaagt tcactcatat ttgtttcaac aactatgtat ggaaaagaat
    19501 cttccttatc tttatacttt atcttgactc tcaaaacatc ctttccttcc tcaaccacac
    19561 tttctatttt gtccatttct ataccatatt tattatttcc tctagtcact ataatgtata
    19621 ttttgttatc gattttagcc gctaatgctc tttcttcatt tgtatactta tccatcacat
    19681 ctaatatctt ttctggtatt gaatttcttt gtaatactgt ataatctatg ggtttagatt
    19741 tctctacaaa catacttggt atgaatataa atccccctat aacgaataca agagctataa
    19801 ctccataaat tataccttta atattaaact ttacattcaa tcttatcact cctcccatat
    19861 attctaaata tatgatttat aaacttatct tagactctaa attattgatt agtatatatt
    19921 tatacataat tataaatata tgtaatgtaa ctaaaaaaca tgttttttct atttcataat
    19981 aaacgagcta aaaaataaaa agagctatat gaaaacattt tcattgtttt catatagctc
    20041 ttgagtttac aatattaaaa aatacttatt tgtcactgat tccttcttga tatccagatg
    20101 tttttgtaat ctgtctataa agaacacaaa ccagtattat gacgcccata tatccagtat
    20161 atggatataa aataccaacc agcttatcaa atggtaggaa gccacctata agacctaata
    20221 tagctgctat taaagttatt aatttaaact taggatgttt atcatctaca aatctattag
    20281 ttactgacca taataaaggt acagctgttg tatatatacc taatataaga attattgaga
    20341 ataaaactcc aattactggt gaaatcttat ctgctaagaa taatgatggt attgcttttg
    20401 aatataagtt tcctatatca gaaagaatag ctaagtgcat cattatagct gcagccataa
    20461 gtgctactcc acctaatata ccaccacaaa tagcttcttt tttgttagca gcacttgctc
    20521 ctaatccagt taagaatgcc ataacaacaa ttgtgttata agtagtatat attacaccag
    20581 atagataggc atttggaact ggtttagcta tctttacagt gctaagaact tgtgcagctt
    20641 ctcctatatt acctatgttt ttagctaaac ttataccacc aactaaaagt gtaaagatta
    20701 ttattatagg ccctattcca cctagtatct ttgataattt atcaagacct agtgaaacag
    20761 ttaataatgc tactactgcc atacctactc tacctacaaa tggatttaat ccatagtatt
    20821 cagttaaagt tgctcctgct cctgaaatca ttactacgaa aacaccaaat aagaataaag
    20881 gtccaaacca ttcaaagaat attcctaaat acttaccaca ataaacacga taaattttga
    20941 taggttcttt aagttgaagc tctttacctt ttgacataac tgaagcaccc atccatgaga
    21001 ataataccat tgaaattatc ccaccaataa tacctgcaaa accataaaaa gagaagaatt
    21061 gcattatttc ctgtcctgtt gcaaatccag aaccgattac tgttgctaca tacgcacctg
    21121 cgaaactgat gactgtttta acatttactt tttctgacat atctcagtta cctccccatg
    21181 tgaaattttt tcttctgatt aaacaattcg ataaactacc tacaatttcc tttaacataa
    21241 aatacatgat aactgtttta tagcttttta gtataataac ttaaaactta taatctgatt
    21301 ttatacccta ctcctcctct cataattttt ttagattctt ttatctaagt agtcattata
    21361 tataaaatta gtttctctat aaattatata ttagtcatat ttttctgaat agtcaatata
    21421 tatttgaata ttttctctat ttttgtaaaa atttg
//
LOCUS       DAYASV010000060        21432 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYASV010000060 DAYASV010000000
VERSION     DAYASV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.060"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            300..1004
                     /locus_tag="ACGI68_002151"
     CDS             300..1004
                     /locus_tag="ACGI68_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138586.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            1006..2493
                     /locus_tag="ACGI68_002152"
     CDS             1006..2493
                     /locus_tag="ACGI68_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0138587.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            2767..4311
                     /locus_tag="ACGI68_002153"
     CDS             2767..4311
                     /locus_tag="ACGI68_002153"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0138588.1"
                     /translation="MFLSDFLGQKEGYIKVVRYAKLGFIICLGFCNLITLNPKITMPL
                     IFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEIVVTSIFVLFVKEGFEIMLENNSI
                     RVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYWLPLFLIPIFSLFLSVSIFDVSKF
                     FSLESLKFLSLISSVGILYINFIVFYLFKFIIDKTKLSMKYELLEKEIIHKEELRLSN
                     ECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFGYNNACKNIFKEIKDKDLVHSEDK
                     AIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRTSITSIYNDENELLRVVGVIVDVD
                     SDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETIVMNTGDKKDALFIIDLDDFKEIN
                     DNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGDEFVVYMQDYVTEVNLHKKAKELN
                     RVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFELFKNADRALYSIKASGKNSYCLFE
                     EELYVQ"
     gene            complement(4674..5504)
                     /locus_tag="ACGI68_002154"
     CDS             complement(4674..5504)
                     /locus_tag="ACGI68_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0138589.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            5577..7304
                     /locus_tag="ACGI68_002155"
     CDS             5577..7304
                     /locus_tag="ACGI68_002155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138590.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7341..9203
                     /locus_tag="ACGI68_002156"
     CDS             7341..9203
                     /locus_tag="ACGI68_002156"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138591.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            9536..10225
                     /locus_tag="ACGI68_002157"
     CDS             9536..10225
                     /locus_tag="ACGI68_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HFH0138592.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTISVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10342..11016
                     /locus_tag="ACGI68_002158"
     CDS             10342..11016
                     /locus_tag="ACGI68_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HFH0138593.1"
                     /translation="MTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMVYKKT
                     YMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDPMDLV
                     FLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNEDSEDI
                     ATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAVLVSY
                     NGDYVA"
     gene            11033..12940
                     /locus_tag="ACGI68_002159"
     CDS             11033..12940
                     /locus_tag="ACGI68_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HFH0138594.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSADLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            13393..14082
                     /locus_tag="ACGI68_002160"
     CDS             13393..14082
                     /locus_tag="ACGI68_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0138595.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            14518..15057
                     /locus_tag="ACGI68_002161"
     CDS             14518..15057
                     /locus_tag="ACGI68_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0138596.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15076..16119
                     /gene="potA"
                     /locus_tag="ACGI68_002162"
     CDS             15076..16119
                     /gene="potA"
                     /locus_tag="ACGI68_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:1902047 - polyamine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0138597.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            16121..16957
                     /locus_tag="ACGI68_002163"
     CDS             16121..16957
                     /locus_tag="ACGI68_002163"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138598.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17062..17736
                     /locus_tag="ACGI68_002164"
     CDS             17062..17736
                     /locus_tag="ACGI68_002164"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138599.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            17736..18788
                     /locus_tag="ACGI68_002165"
     CDS             17736..18788
                     /locus_tag="ACGI68_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0138600.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            19018..19866
                     /locus_tag="ACGI68_002166"
     CDS             19018..19866
                     /locus_tag="ACGI68_002166"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0138601.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            19856..>21432
                     /locus_tag="ACGI68_002167"
     CDS             19856..>21432
                     /locus_tag="ACGI68_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HFH0138602.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYIGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     LTKSDTRFKSISNISKV"
BASE COUNT         8404 a         2160 c         3716 g         7152 t
ORIGIN      
        1 tttatcaaaa atatgcaaag tactacacct tcaaatatca cctaaagtaa ttaaaaggca
       61 attaaggtga agtagttcaa gtatttctaa aataagttta ataaattcta taataattta
      121 ttttttgcct taaaattgcc ttaaaatcca aacaatttta aaactaatgt actataaaaa
      181 gctggtataa tttaaatata gtaaaaaata agatttatct tatctattgg gatttaatta
      241 tttaatcaat atatttatac catttaattt aaataaaaag tctattaagg agtgagctta
      301 tgagtaagtt aatttatata gcagatgatg aagataatat aagaaatttg gtaaagacat
      361 tcttaaaaaa tgaaggtcat gatgttatgg attttaaaac gggtgatgaa cttttagaac
      421 aatttaatat aaaggagtgt gacttagtaa tactagatat aatgatgcct ggctcaagtg
      481 gttttgaagt atgtacaaaa cttcgtgaga aaagtacagt cccaataatc atgcttactg
      541 caagagatac agatatagat tatattacag ggataacctt aggtagtgat gattatttta
      601 caaaaccatt tagcccaatg tctttggtta tgagagtaaa atctatattt agaaggatag
      661 agtttgaaaa gaaacaaaat tatgataaat atagtaactc gatagatatg gaattaaaat
      721 ttggtgatgt aataataaat aaaaagaata aaatcgttac aagtaaaaat gtaaatatag
      781 accttacacc aaatgaatat aacctattga cttatttgtt tgaaaatata gatagagcag
      841 tttccagaga tgaattatta aacaaaatat ggggatacga tatagaagtt gagacaaggg
      901 ctgctgatga tacagtgaag agattacgta aaaaaatttt aaatacaaat attttaatag
      961 aaactgtatg ggggtttgga tttaggttga aggagaaaag ttaagatgaa gaatggaaat
     1021 gacaagaata gaattggaaa gattaaatcc aaatttaaat taaagttctc aaagaaaaat
     1081 ataagcataa aaactaggat tttgttgagt atactttgtt tgataattat agtatttatg
     1141 ataatattag tatcttttaa tgtatttctt gatacatata taaaaacaac tgcgaatgaa
     1201 gaattgacaa agtctacaga aatagtagaa catatggata gtaattttag acctattaag
     1261 ccaccacaag atgatggaaa gcttcctccc aaagtattat ctaattttat gagaaatgta
     1321 caagataaag ttaggatggc agaaacacaa tcagatgctg atgctatggt agttgattct
     1381 aagtataacc tgatatttcc aacaagagag gatgatttct taaaaaatgt tgatgagatg
     1441 gagctgatta gggagtgtat tcaaaatgaa aaattagatt taaattcaga tgaaaataca
     1501 cggatatcta ccagaaataa ggattattat atttcaactg taaaaattac tagtatatat
     1561 ggtgaacaag acaagtatct tatacttttt atagacatat caaaaactct aaatctagca
     1621 caaaagataa atattgtatt aatatctgta atgtgttttg ctggaatatt agcaatattt
     1681 acagctgtga ttttatctga gaaaattgct aaaccgataa aagagttgtg tgagtttgca
     1741 aaaaaaatgg gtcaaggaga tttcaaaaga acctactttg acttttcaga taaagaatta
     1801 gtagagctct caactgttat gaataagagt gctgagtatc tggataagta tgataatgaa
     1861 caaaaaatat tttttcaaaa tgcatctcat gaattaagga caccacttat gtctattaaa
     1921 ggttatgcag aagctataaa atataatgtt atagatagta aacatgcaag tgatataatt
     1981 cttgaagaaa gtgataggtt aagtgatatg gttgaagatt tattatacat ttctaaaata
     2041 gataatataa ctaaagattt tgaacttgta gaatgtgatt taagagaagt actttcaaat
     2101 tgtggagcta ggcaaaatgt aagagctata aataagggga taaaatttat ttatgaattt
     2161 gatgaagaaa tggttttatt tgaatgtgat gaaaaaaaca tttccaaagc atttatgaat
     2221 cttatagaaa atgctttgag atacgctaag agtgaaataa aaatagtgtg taagtataat
     2281 caaaaaaata tagttgtgat aatagaagat gatggaattg gaatcaagaa agaagattta
     2341 cctcatgttt ttgaaagatt ctataaggga gtagggggaa atcatggaat tggattatca
     2401 atagttaaat ctattgtaaa taagcatggt gggcgtatat acgtagaaaa tggcaaaaaa
     2461 ggagctaaat ttactatatt atttaaattg taaaaaaatt agagaaaata tagagatgtt
     2521 tgaagttgtt ttttatatat atctgttgta tactgttgaa aaaagaacaa tattaagctg
     2581 acatatatta cgatagttga catttaaagt aaatgcttaa atatagatac gaaaggaaaa
     2641 gtaaaatctg aagtgtattt aggtttaagt aagcttctta ttttataaaa aatatgtatg
     2701 aaagaacagc tgatttttaa tattacaaat ttagtaacgg ttgtatttga agcttttgtc
     2761 atccatatgt ttttatcaga ctttttaggt cagaaagaag ggtatataaa agttgtaaga
     2821 tatgctaaac tgggttttat aatatgttta ggattctgta atttaataac tttaaaccct
     2881 aaaatcacaa tgcctttaat tttcatcctt attttttcaa catcattttt atataaagga
     2941 aatttaaaga cacgattgtt tactactgtt ttattaagta tattctttat actgtcagaa
     3001 attgtagtta caagtatttt tgttttattt gtaaaagaag gctttgaaat aatgcttgaa
     3061 aataatagta taagagtatt ggcgacaatt ttatctaaaa ttgtcttttt gttgacttgt
     3121 aaaattattt gtctatttaa aaaggatgta catttagata tgcccataaa gtattggctc
     3181 cctttatttt taatacctat atttagttta tttctgtctg tttctatctt tgatgtaagt
     3241 aaatttttta gtcttgaaag tttaaaattt ctaagcttaa tatctagtgt tggtatttta
     3301 tatattaatt ttattgtctt ttatttattt aaatttatta tagataaaac taaactatct
     3361 atgaaatatg aactacttga aaaagaaata attcataagg aagaattaag acttagtaat
     3421 gagtgttata aaattattgt tgaacagact gattctgtcg ttttcgaatg gaatatcaaa
     3481 gaaaataaat catttgtatc gcaggcttgg actgaaaaat ttggatataa caatgcatgt
     3541 aagaatattt ttaaagaaat taaagataaa gacttagttc attctgaaga taaagctata
     3601 tttgaaggtt ttcttgaaag tattaaaaaa aagaatatgc acaatcaagc agtatatcgt
     3661 ttaaaaaaat ctaatggtga atatatttgg tgtagaacat caattacaag catttataat
     3721 gatgaaaatg aacttttaag agttgtcgga gtaattgtag atgtagatag tgatataaaa
     3781 aaatatgaag agttaagaac tagagcagaa agtgactcat tgaccaatat atacaataaa
     3841 ggtacatttg aaaaattagt ggaagaaact atagttatga atacaggtga taaaaaagat
     3901 gcattattca ttattgattt ggatgatttt aaagaaataa atgataattt tggtcatcca
     3961 tttggtgatt ttgtactaaa aacatttgct gataagatac aaactagttt tggtagtaaa
     4021 gacttagtcg gaagaattgg tggagatgaa tttgttgtat atatgcaaga ttatgttaca
     4081 gaagtaaatt tgcataaaaa agctaaagaa ttaaatagag tactttcgga taattacact
     4141 gatttgagtt ttagttttga tgcttctgtc agtataggta ttgcaaggta tcctcaagat
     4201 ggaacttcat tttttgaatt gtttaaaaat gcagatagag cactttatag cataaaagca
     4261 tcaggtaaaa attcatattg tttatttgaa gaagaactat atgtacagtg aatttgaatt
     4321 tagattttat gtgagagtaa gatttatata tacttgtggc tgtctgattt ataaaatatg
     4381 ttactttgta tatttttata tgtttcagta aggttaatta acatgagcaa aggtgtaagt
     4441 aaatttatat tagtttaagt ttacttacac ctttataaaa atacaaataa tagattcatt
     4501 tacattctta aatgattaaa gctctttacc ttctaaacat gcataacata tcgttataat
     4561 tccagtaaat aagactaaaa tgcccattac taagattaca atttcatcat ctattactcc
     4621 aaatgttttt aaagtaactt atttcgcttt gtatcataat aatttgtctc tttttacatt
     4681 ttaacttctg ctgagatttg agttagatat tcattagaat ctcgcacact tatatcatat
     4741 agaagatatt cttcataaac aaaatcccca atacgtaaat tattctgatt aaaaaaattt
     4801 aataactttt cataagcctt gtaaatagtt tcataactcc ctttatgata agcagttaca
     4861 taaagtccct ttggtttgat tgatacagga tatttcttgt actctttatt aacttttgta
     4921 aataaatatg aatatgattc aaagtcttta ttaattatat cttcaccttt aatgattgag
     4981 ccaatcgtac cataatcact caattcatag ttgtccataa agttaataca ttcatataaa
     5041 aaattttctt cctcatctcc taaaaactgt tctttatatg taacaggtgt tttgaccaag
     5101 tactcttctt catgttcttt taaagtaata gtattcgcat ctatattaca tgcactttta
     5161 gtaaatgaaa tagtagattc cattagagct tgaatattat ttagtttttc aatttcattt
     5221 ttaatatcaa tggttttctt ttctaaaagc tcaattagta aattaggtgt tcttttgtct
     5281 atataagctt taatttcttt taaaggcatt tttacttccc taagtgaaga aattatacca
     5341 aaaatgctaa gttgatggta tgagtagtat ctatatccat tttctttctt taattctggt
     5401 gagaaaagac ctatatcatc ataatggaaa agtgtttttt tattgattcc acaaatcttt
     5461 gcaaattctc cagttgtaaa ataatttttt gaaacttgac tcataattat aattcctcct
     5521 tgactatcct gttacagtat agtttatcat agtattttgt aaaggagtgt gattatatga
     5581 aaaaattaat tcatttttta aaaccatata gagttttaat tgttgtagtt ttgatattta
     5641 cgtttttaca aacacttgga actttgtata taccaacact tacagcaaat atagtaaata
     5701 atggtgtagt aaagggagat atagattata ttgtaaaaac aggtttaatg atgatgatag
     5761 tagctggtat aacagcttta tcagctgtgt tagtttgtaa agtatcagca aatctttcat
     5821 ctggattttg tagagatatt agagaagcag tatttataaa atcacaagat ttatcaataa
     5881 atgattttaa taatataggt acagcatcaa tgattactag gagtactagt gatattactt
     5941 taataggtca gtctgtgttt atgtttattc aattagtatt accagcacca attataactg
     6001 tttctggtct attcttagca tattctatag ataaagcaat gacaattatt attgttgtag
     6061 taatgttttt attcatgctt tcagcatttt tagttggtaa aaagcttata aagttgttta
     6121 aaatgatgca aataaagatg gataatatga atagggtgtt acgtgaggta gtaactggag
     6181 taagagttat aagagctttt aataggagtc gttttgaaaa aaagagattt gatagaactg
     6241 caatagatta ttctgaaaca gcaatttcaa taaataaaat atttgcagta cttatgccta
     6301 ttgtaatgtt aataatgaat ttaggtatag taagtattat ttggtttggt gggatgagag
     6361 tcagtaatgg aaatatggaa ataggtcata taatggcact tgttgaatat tgtatcttaa
     6421 ttttattcta tttaatcatg ggagtaatgg tatttatgta catacctaga gcaggtgcat
     6481 gtgcagatag agtaaatcag atattagata tagaacctga aattgtagat ggaaatggtc
     6541 ataaagatac tgtctcagag agaggacatt tagtatttaa aaatgtaacc ttttcatatg
     6601 cacaatcaga agaacctgtg ttaaataata taacatttga agcaaaatct ggagaagtaa
     6661 ctgcaattat aggaagcact ggctcaggca aatctactat tgcaaatata ataccacgtt
     6721 tttttgaaat tcaatctgga gaaatatcta taaatggtca ggatattaag aagattcctc
     6781 aaaaagaact tagagataag attggatttg taccacaaaa agctttttta tttagtggaa
     6841 caatagaaga aaatataagg tatggaaaag aagatgcaag tattgaagaa gtaaagcatg
     6901 ctgcaagtat agctcaagct gatgaattta tttatgatat ggaagacaag tatgactcat
     6961 ttgtagcaca aggtggaaat aatttgtcag gtggtcaaaa acagagaata tccattgcaa
     7021 gagcgttagt tagaaagcct gaagtatatg tctttgatga tagtttttct gcattagatt
     7081 ttaaaacaga taaaagactt agaaaagcgt tgaaaaatga aatcaaagat tcatcagcta
     7141 taattatagc acaacgtata agtacaatca tggatgctaa tcagattata gttttaaatg
     7201 atggaaaaat tgtaggtatt ggaaaacata aagatttact tgaaaattgt gaagtttata
     7261 agcaaattgc ggattcacaa cttagtaaag aagaacttgc ataaaatgaa gaattaatct
     7321 gatatttgga ggtgtatgat atgagtgaaa aaagaataga tgaacaagat atagaagtac
     7381 ttagttttga agatggcatg tcagcagaaa aagctaagga tgcaaaaaaa actactaaga
     7441 gattattaaa atatatggca aaacaaaagt ttaaaatatt aattattttt atttcagtat
     7501 tgataagttc agcgcttaca gttttagcac ctatggttat gggaaaagct attgaccaac
     7561 tatttaatgg aataaaaact gcagtccaaa caggtacaaa attcagtgtg aattttagta
     7621 ctatgggagg gatagtatca atacttctag ggttgtactt aattagtgca gtatttatat
     7681 atattcaaca gtatattatg tcaggagttg cacaaaatct agttcttagc atgagaaaag
     7741 atttaagtga taaactcaat aaacttcctc ttaagtacta tgattctcat aaaaaggggg
     7801 aaacacttag tatagtaaca aatgatttag aaaaagtagc agatagttta caagaaggat
     7861 taatgcaact tattacagca gtagttacag tagttggttc tattgtaatg atgataagta
     7921 taagtgtacc acttacaata gtatcagcta taacattact tgtttcattg ggtattactg
     7981 ttgtaattgc aagaaaatca caagaaagat tctctgaaaa tcaaaaggca ctaggagagt
     8041 taaatagcaa tatagaagag atatttactg gtcaaatagt ggttaaagca tttagtaaag
     8101 aaaaggatac tataagaaac tttaaaatat taaatcaaaa tctttataat gcaagtagaa
     8161 aagcacagtt ttcgtcatat gcaatatctc caattattag atttataaac ctaattggct
     8221 atgttattat agcagttgta ggtgggatat ttgcatcaac tggagcaatg actcttggta
     8281 gtatacaagc gtttattcaa tatgtaaatc aagcatcaga gcctactaca gaaatatctt
     8341 atattgtaaa tatgcttcaa gcagcaatag catctgcaga gagagttttt acagttatgg
     8401 acgaggtgga agagataaag gatgaagaat cttcaaaggt tattagcatg cctaaaggta
     8461 aagtacaatt tgagcatgtt aaatttggat atagtgatga ttttattctt atgaaagata
     8521 ttaatatcaa tctaaatgca ggagataaga ttgcaattgt tggacctaca ggagctggaa
     8581 agacaactct tgtaaattta cttatgagat tttatgagat acaaggtgga agaattacta
     8641 ttgatggtgt taatataaaa gatttaaagc gtggagaact tagaactatg tttggtatgg
     8701 ttcttcaaga tacatggctg tttaatggaa gtataaaaga aaatattgca tatagtaagt
     8761 cagatgccac aatggatgaa attgtaagag cagcaaaatc agcaagagta gaccatttta
     8821 taaggacttt accacaaggg tatgacacta tattgactga ggatgcttct aatctatctc
     8881 aaggtcaaaa gcaacttcta acaattgcca gggcaatact ttcagaccca tcagtattga
     8941 ttcttgatga agcaacatct agtgtagata ctagaacaga ggtagaaatt caaaaggcaa
     9001 tgaataactt gatgaaggga agaactagtt ttgttattgc acatagatta tcaactattc
     9061 gagatgctga tttaatatta gtaatgaaag aaggaactat tattgaacaa ggaagccata
     9121 aggaacttat tgaaaagaaa ggattctatg aagaacttta taacagtcaa tttacaagtg
     9181 aatatgatga agatgttgta tgattatcaa gttttaggca actaaatgat tgataaatat
     9241 taagttaaat atagtaacat gtaaggttat atggaataag agtatttctc tatttcatat
     9301 aacctttttt atttatcaaa ataagataaa atttcctttc taaattagat tgaagaatgt
     9361 atttatcaat ataaaagtaa aataatcatt attattagtg aagtaaatat tgtttgccac
     9421 atatatgcca taaagatgca cttattttac ggagatttaa caaagtagtg taaagtatac
     9481 tctaaatata aaataaatca aatagagtta aataaattga gaggtgatat aaatagtgaa
     9541 taaaaaattg aactatagat ttgaaatgaa gcacaaaata actgaagcag atgtgttagc
     9601 tttaaaatca agactctatc caataatgaa gaaagatgaa aatgcatcta aagatggaaa
     9661 atatcttatt aggagtttat attttgatac tcctgaagat aaagcacttt tggataaatt
     9721 aaatggcgtt gcaataagag aaaagtttag gataagattt tataataatg actgctctta
     9781 tataaggtta gagaaaaaaa tcaaacatta caacatgaca tcaaagctat caactaattt
     9841 aaccaaaaaa gaagtaataa atatactaaa taatgatatt gaatttctaa aagagtcgac
     9901 aaattgttta ttaagagaat tttatttgaa attaaaatgt gaaagattag aggcaaaaag
     9961 catagttgat tacaatagag aagcttttat ttatccagtt ggaaatgtaa gagttacaat
    10021 cgatagtgat attaaaactt ctgtaaattc ggtagattta tttaataaag atttgccaac
    10081 tatttcggta attgatgaga atatgactgt attggaggtt aaatatgatg aatttatacc
    10141 agattttata aaggatttaa ttcaaattaa taagactacc tctacagcag tttcaaagta
    10201 tgcttcaagt agattatata tttaagaata aaggctatga tttatataaa gatatacata
    10261 aataaggtta aaaaacaaat aagttaaaaa taaataaaat aaaaaataaa taagttaaaa
    10321 ataggaagga tgattttaat tatgacattt aatgatattt ttaaatcaag ttttatagaa
    10381 aacgtatcag gtttttcatt tgtggacagt gctttagcac taggttcagc ttttttagta
    10441 ggtttgttta tatatatggt ctataaaaag acttatatgg gaattatgta ttctcgacca
    10501 ttcaatgtgt cgcttgtagc acttacaatg cttacaactt ttgtaatatt ggcggttact
    10561 tcaaatgtag ttttatcctt aggtatggtt ggtgctttat ctatagtaag atttagaaca
    10621 gcaattaaag acccaatgga tttagtattt ctattttggt cattaggttc aggtatagtt
    10681 ttaggagcag gtttgattcc cttagctgtc atgggttcaa taattatggg attgattcta
    10741 atattctttt caaataaaac aatatctgaa actccatata tattgatggt aaattgtaat
    10801 aatgaagatt cagaagatat agcaactgat aaaattaaaa aagtatttac taaatatcaa
    10861 ataaaatcaa aaagtgttac accagaaaaa ggaatagaat tagtgtttga agtaagaatg
    10921 aaagatggag aaacaagtct tataaatgac ttgagtcaag tagatggagt tactaatgca
    10981 gttcttgtaa gttataatgg tgactatgta gcatagagag gagtaatctt ttatgaaaga
    11041 taaaaaattt acccttctta tctcgattat gattatattt ttatgtgctg tagttggagt
    11101 ttatagtaca tctagcaaca aaagtgctga tttatatagt gatgtatata ttgaaaaata
    11161 ttttaacaga gacaaggtta tggaagttaa tatagagata gatgaaagtg acttgaagga
    11221 tatgaatgaa aatgctataa aagaagaatt taaggttgca aaagtaactg tagatggaga
    11281 tacatatgga aacgtaggta taagaactaa aggaaattca agtcttatat ctgtagcaaa
    11341 tagtgatagt gatagataca gctataagat taattttgat aagtataata ctagtcaaag
    11401 tatggaaggg cttactcaat taaatcttaa taactgttac tctgacccat cttatatgag
    11461 agagttttta acatatagta tttgcgagga aatgggatta gcgactccag aatttgcata
    11521 tgctaaagtc tctataaatg gcgaatatca tggtttgtat ttggcagtag aaggattaaa
    11581 agagtcttat cttgaaaata attttggtaa tgtaactgga gacttatata agtcagatga
    11641 aggaagctcg ttgcaatata aaggagatga cccagaaagt tactcaaact taatcgttga
    11701 aagtgataaa aagacagctg attggtctaa aatcacaaaa ctattaaaat ctttggatac
    11761 aggtgaagat attgaaaaat atcttgatgt agattctgtc cttaaaaata tagcaataaa
    11821 tacagcttta ttaaaccttg atagctatca agggagtttt gcccataact attatttata
    11881 tgagcaagat ggagtatttt ctatgttacc atgggatttt aatatgtcat ttggtggatt
    11941 tagtggtttt ggtggaggta gtcaatctat agcaattgat gaacctacga caggtaattt
    12001 agaagacaga cctctcatat cctcgttatt aaaaaatgag acatacaaaa caaaatacca
    12061 taaatatctg gaagagatag taacaaaata cctagattca gactatttag agaatatgac
    12121 aacaaaattg catgacatga tagcatcata tgtaaaagaa gacccaacag cattttatac
    12181 ttatgaagaa tttgaaaaaa atataacatc ttcaattgaa gattctagtg ataataaggg
    12241 atttggtaat aaagggtttg acaacaataa ctctaataac agtgattcta ataataattc
    12301 taatagtgaa aataagcgct ctggaaatca aagtgatgaa aaagaagtta atgctgaatt
    12361 aacatcaagc gtagtcaaag ctaatacaga taatgaaact aaaaataaaa ctacaaatga
    12421 tagtgaaagt aagaataata cagataaaga taaaagtgga aatgataata atcaaaagct
    12481 agaaggtcct atgggtaaag gaggtaagtc aataccaggg gttttggaag ttgcagaaga
    12541 tatgagtaaa actataaaat ctcaattaag tggagaaact tcttcgacaa agcaaaactc
    12601 tggtgatgaa agttcaagtg gaattaaagg tagtgaaaag tttgatgagg atatgagtgg
    12661 tatgccagaa ccacctgagg gaatggatgg taaaatgcca ccaggaatgg gtaatatgga
    12721 taagggagat atgaatggta aaaatggcaa tatgaatatg gatagaaatc aagataatcc
    12781 aagagaagct ggaggttttg gcaatagagg aggaggctct gtgagtaaaa caacaacata
    12841 cttcaaatta attttaggtg gagcttcaat gataataatg tcgattatgt tagttggtgt
    12901 atcaagggta aagagaagaa gatttataaa gtcaaaataa gattaaaatt gaatataaat
    12961 aattttaata ctgttaagtc taaatattta cttagcagta tttttttaga aaataatgta
    13021 tttggttaat aatataaggt aataaattga taaaaattaa atacagcata ttaataatac
    13081 ataataaagt aataaaaaaa ctgtttaagt ataataactt atatttagac agtttttttc
    13141 atttataaat aatataaatg aaattgaaaa catttgaagg atagaaattg gttggataaa
    13201 tgtataaatt acttcaccaa aaaataatga caaaaattgt aactaagttg taataaaatg
    13261 gtagttattt agcataaaat aatgtatcat agaagtatat tataaaatta ataaaataag
    13321 atttttgtaa gaaaactgct ggcttttgat atttagagtg agtagttaaa ttatgtttag
    13381 gaggggtaga atttgttgca aaaagtatta tggaaatttt taaagctagt agtcggttta
    13441 tttatatgtt cagttggtat agtattgacg ataaattgta accttggatt atctccctgg
    13501 gatgtatttc atcaaggact ttcaaatcat ataggaataa ctataggaac tgcttctata
    13561 attgtaggct ctatagtagt tattgctgat gtcgttcttg gagaaaatgt aggatgggga
    13621 actgttttta acatgctact aattggattc tttatggatt tacttttata tagtaattta
    13681 attccagagg ctgatagttt atttgtagga ataattatgt tgatattagg attggttctt
    13741 ttgtctgttg gaatggtatt ttatatggga agtggtttag gaagtggacc tagagatggt
    13801 ttgatggttg caattcaaaa gaaaacagga aaatcactta aattaataag aggtactata
    13861 gaagttggag cattaatagt aggattttta ttaggtggaa aagttggtat aggaactata
    13921 ataagtgcat ttggattggg gtatttcaca caaatggtat ttggcttatt taaattagat
    13981 tgtagtaaga ttaaacatag atttatagta gatgatataa aatttatcaa agtgtatatg
    14041 agtggtgaga aaaaagctgc tgttcaaaat atagttaagt aatttaagaa taaattcaac
    14101 gaaatgttaa gtattttaaa ttaacatttt gtttagatag ataaattgaa tattaaaaga
    14161 atattaaaat atgaggattg ctagaacact ataaaatata gatttctagc aattttttat
    14221 ttgttcaata tttttttagt actagaagtc ttattctcta tttaatgaat gaattaattt
    14281 ataattagag atactaaact ttatagatat taatataaac acctttaaac tgtgtatttt
    14341 ccaaagcctt taaaatgctt gtatttattc taaataaaaa aataaaaaaa tgtcaaaaaa
    14401 actcttgtat tatatccaaa ttttatatat aattacctat gttgtttaga aattttaaac
    14461 tcaatgttta gtaaacaaaa tgtttagtaa aataaaacag aaactaggag gtgcaacatg
    14521 gatataggtg aaaagataaa aagattaaga acagaaaaac aactgaccca ggaagagtta
    14581 gctaatagat gtgaattgtc aaagggattt atttctcaat tagaaaataa cttaacatca
    14641 ccatccatag ctacccttat agatatactt gaaatactag gtacaaattt aagggaattt
    14701 tttaatgaaa tagatgatga aagaatcagt tttacaaaag aagatatgtt tgaaacagaa
    14761 gatgaagatt taaaatataa attaaagtgg ttaattccaa attcacaaaa aaatgaaatg
    14821 gagccaataa taataacatt gtatccagga ggacagtata aagaagaaaa gccacatgaa
    14881 ggtgaagaat ttggatatgt attagcaggt tcaatatatg tccatatagg cgaaaagaaa
    14941 aataaagtaa aaaaaggaga gagtttttac tttagaccga aagcaaatca ctatatatca
    15001 aacgagggca aaactacagc aaaagtgatt tgggtaagta cgccaccatc attttagaat
    15061 agaggtgttt aataattggt agaaaatata atagaattaa gggatatatc taaacattat
    15121 gaagaactta ctgttttaga taatttcaat ttagatataa agaaaaatga gtttctaaca
    15181 ttacttggac caagtggatg tggaaaaact acaacattaa aaataatagc aggatttgag
    15241 tatgctgatg atggtaaagt tttatttgaa ggtaaggaga taaataattt gccaccatat
    15301 gaaagacaag taaacacagt atttcaaaaa tatgctttat ttccacacat ggatgtttat
    15361 gaaaatgtag cttttggatt aaaaattaaa aagactccaa aagatgtaat agatgctaaa
    15421 gtaaaagagg ttttgaaact tgttgcacta gaaggttttg aaagacgaca agttgaatca
    15481 ttgagtggag gacaacaaca aagaattgca atagcaagag ctctagttaa tgaaccaaag
    15541 gtattattac tagatgaacc acttggagca cttgatttaa agttaagaca agagatgcaa
    15601 atagaattaa agagaatcca aaaaaaatta ggaataacat ttgtttttgt aactcatgac
    15661 caagaagagg ctcttactat gtcagatact attgttgtaa tgtataaggg caaaattcaa
    15721 caaatgggaa caccacaaga tatatacaat gaacctaaaa attcatttgt agctaagttt
    15781 ataggtgaaa gtaacatatt tgatggaatt atgatagaag attataaagt taatttctgt
    15841 aatagagatt ttgagtgtgt tgataaagga ttcgaaaaaa acgaaaatat agaagtagta
    15901 ataagaccag aagatataaa aatggtaaag ccagaagaag gaatgttaaa ggggaaagtt
    15961 acatctacag tgtttaaagg agtgcattac gagatagaat tgaatgaaaa tggaagattg
    16021 tggatacttc ataatactaa aaatgctgag gttgggactg aactaggcat ggatatatat
    16081 ccagaagata tccatataat gagaaaagag aataactaag atgaaaagaa aatctttttt
    16141 agcatatcca tatgtagttt ggagtgcgat atttgtaata attcctttaa tattagtagt
    16201 attttttagt tttacaaaag aatcaggtgg aggttatgcc tttacacttg agaattataa
    16261 agaagtaatt gaccctatat atatgaaagt ttttgggcgt tctattcttt tagcaggagg
    16321 agctacatta atatgcctta tagttggtta tccagtggca tatataattt caaaggctcg
    16381 tgtaagtaga agaggttcat taatattatt gtttattctt cctatgtgga tgaactttct
    16441 tttaagaaca tatgcatggg ttgctatatt aggaaaaaat ggacttttaa atacattttt
    16501 aggttggttt ggaatacaac cattagcaat tttgtatact aattttgcca tacttttagg
    16561 tatggtgtat aactttttac catttatggt tcttccaata tatacagcac tatcaaagat
    16621 ggataatgat ttgataaacg ctgctcatga cttgggtgca aataatatga cagtttttag
    16681 aaaaattata tttcctttga gtttgccagg tgtaatgtct ggaataacta tggtatttat
    16741 gccagcagtc actacttttg caatatcaag actgttaggt ggaggtaaaa taatgctggt
    16801 tggagatttg attgaacaac agtttacagt tgttggagat tggaactttg gttcagctat
    16861 atcgatattt atgatgatag taatattaat atctatgtct ataatgtcaa aatttggaga
    16921 tgaatctgac aaagaaggcg gtgggttact attttaaaat taaagaaaat aacttctaat
    16981 atatatttag cattagtatt cttatttttg tatgcaccca tatttgcttt ggtactattt
    17041 tctttcaatg attcaaaatc aatggcaaga tggaatggat ttacatggaa gtggtatggt
    17101 caattacttc aaaatgagag tataatgagt gctttatatt atactattgt aatagctata
    17161 ttagcgtctg taatatctac aatagtagga actataagtg ctataggaat acataagatg
    17221 agaggtaaga gtaagaaatt aatactaaat gtaaactatc ttcctatttt aaatacagag
    17281 atagttacag cagtagctct tatgagtctg tttgtatttg taaaaatgga atttggattt
    17341 acaacaatgc tattggcaca tataatgttt tgtttgccat acgttatact ttcagttttg
    17401 ccaaaaatga agcaattacc tgataatata gaagatgcag ctatggactt gggagcaaca
    17461 cctatatacg ctcttagaaa agtaatttta ccacaaataa aaccaggtat agtttctgga
    17521 tttttaattg catttactat gtcaatagat gactttataa taagtttctt taatgctgga
    17581 aatggtgtaa gcaatctttc tatagaaatc tatggaatgg ctagaagagg tataaaacct
    17641 gaaataaatg cattatcaac aataatgttt gcagtggttc taggtttact tttactagct
    17701 aataaaaaag aatctatagt aagggggatt aaataatgat taagtttaaa aaagcaatat
    17761 cccttatagc tgtaggtatg atgacagtta ctttatttac aggatgtaat aaagcaactg
    17821 attctacaaa agttttaaat gtatataatg ttggagatta tatagatgag tctcttatag
    17881 ataagtttga aaaagaaaca ggaatagatg ttcaatattc aacttatgat actaatgaaa
    17941 tgatgtatca aaaagtaaag agtggtagta cacactatga tttagtattt ccttcagact
    18001 atatggtaga gaaaatgaaa aatgaaggtc ttttggaaaa attagatttt aagaatatac
    18061 ctaatatgaa atatttagat aagagttttt taaatcctgt atacgacaaa actaatgaat
    18121 atagtgttgc atatatgtgg ggaactcttg gaatactgta caataagaaa gaagttaaag
    18181 accctatgga tagctggaat atactttgga atccaaagta taagggaaat ataatgatgt
    18241 ttgactctgt tagagatact atagggataa ctcttaaaaa attaggatat agcatgaata
    18301 gtgtaaatcc aaaagaaata aatgaagcta aaaatctttt aatgaaacaa aaggatttag
    18361 tattggctta tgtaaatgat gaaggtaagg atagattgct tggtggagaa gtggcaatgg
    18421 gtatgctgta ttctggagat gcagttacac taatggagca aaatcctgac cttgattatg
    18481 ctatacctaa agagggaact aataaatggg tagatgctat gtgtgtacct acaacagcac
    18541 aaaataagaa agaagctgag ctgtttataa actttttatt agacccagag aatgcaaaag
    18601 taaatgctga gtacattggg tattcaacac ctaatacagg agcactaaaa ttattagacc
    18661 cagaaataac tcagaatcct gttgcatatc catctaaaga agtgcttgat aaatgtgaaa
    18721 cttttgaaga tattggtgaa aatataaaat tatatgacaa ggcttggata gagttaaaat
    18781 caaaataggg tcttgttggt ttaagtaaat ttacttaata aatacttaat aaaactgttc
    18841 ttttttagaa aatttatttt ctaattattg aacagtttta ttttttgttt aagttatatt
    18901 catatgcaat ttaaatagtt tattaataaa tgaaaactta tttgtataat gactttaata
    18961 ataattagaa aaaatgggca acttaataga ataattggag gtgtaaattt ataaaaaatg
    19021 aataggataa ataaaaaaat agatttatta atattggctt tgctcgtttt tatattttta
    19081 attgttggaa tagtaatact atcgattagg acagaaaata aattgaatgt gttcataatg
    19141 ttagcaataa ttttttttat aataatgtta acttatttat caaattcagt agtggggttg
    19201 ataacaagtt caataattat atttatgtat acatcctata ttttatataa caatattact
    19261 cacaatatgg atgttgaatt tatatcatat atgtggataa tagcaacacc agtatcttca
    19321 attataatgg gtaatctgaa caaaagcata aatgaattgc aaaatacaaa taaaaaacta
    19381 tctgaacagt ataaggaatt agtgactatt gatagtgaaa ctggacttag aaatttaaag
    19441 attttttata atgatgtaaa tatggagata agtaaatcta ttagacataa tactgatttt
    19501 tctcttatga tagtaaaatt accatactac ggaaacctac agactatatt tggtgaaaac
    19561 aaaactaata aaatagtaaa atatattggt tcaaatatta tagaatgtac aagaaatgaa
    19621 gatataattt atagtttaca aaaagatatg atagggatac taatgccaaa tacatctttg
    19681 gaaggttcaa aagttgttaa agacagaata aagaaaagga taaaagaatt aaatttagat
    19741 ttaaacaata ggggaaaata tgtaaatata gatgtcaaaa ttgcattttt acaatataag
    19801 agtagttttg gagatagtat aaattttaag aatatagttg aagaggaact tcaatatgat
    19861 gtttaaaata tttaataaaa gtctattaaa attagtagtt gtattatcaa tagtactgac
    19921 aatatcaaat tttagcttat cttcatatgc atcagaaatt acttccagca cagataaggt
    19981 attaatcata tatgattcta agaaagaaac agcttataat agagatattt taaatattat
    20041 gaggactcta ttgggaagat ttagttcaga tatagagcta ttaaaattga gtaactatga
    20101 tggtgagata aataaaaatt attacagtca tatatttatt ttaggaatca atgaaaactc
    20161 atataataat gataaaaaca ctaaaaactt gattagttcc ttaaatttat ataaaggaac
    20221 tatatgttgg ttaggatatg gtatagaaaa tcttttggaa cataaaaaat acaatttaga
    20281 ctatgtagga aaaacaaaca atatagttag tgtaaattat agaggtaaat catataactt
    20341 ggatgaacat tatgttttta acattgtaga atcaaaagat acaagtaata aagtaatagg
    20401 aagtattaat gataccttaa ataagtatcc atatataata aatgataaaa atctttttta
    20461 tgtatcaaaa ttagatttag atggagtttt attttatata ttttgcgatt cacttaatga
    20521 tatttttaat attaagacgt ttgataaagg acgtatattt gtaagaatag aagatgtaca
    20581 tgcttttaga gaaccaaaaa atcttgtaga gatagcggat tatttaagta gtaagaatat
    20641 accatttacg attgcattaa taccagcata tgtaaatcct aaaaatcata aggtaataac
    20701 tttatcagaa agccctgaaa ttgtcaaagc aataaaatat atgcaagata aaggtggaac
    20761 tgtgatattg catggatata ctcatcaata caagaaagaa gaagtttctg gagaagggta
    20821 tgaattttgg gatggtaaaa aagatgaacc cttaaaagaa aatatgaaga tatttgtcaa
    20881 agatagagtt ttgaatggtc ttagagtatg tatagagaat ggaatttatc ctcttgcatt
    20941 tgaagcgcca cattatgcaa tggaatcaga gggatataaa gaattaaaaa aatatttttc
    21001 tacttatata gggcaacatc aaaataatga taaaaaattt agcacaaata catatcctta
    21061 tattattagg gatacagagg agtttaatat atttataccc gaaaatttag gatatataga
    21121 cccagaagat aagtttactt ttcaaaatat aaaagaaaat ttagataaat tatccattgt
    21181 aagaggtttt agtggagggt tcttctttca ttcatattta aacatagagt atctaaaaaa
    21241 cacaatagaa tatttagaaa aacagaatat agaatttatg aatttaagag attttaataa
    21301 ttgggtaaaa gttgatgaaa tacaaataag aaataatgga gatgaaatta tagtcaacta
    21361 tgataaggac ttagatgaac taactaaaag tgatactaga tttaaatcca tatccaatat
    21421 tagtaaagtt tt
//
LOCUS       DAYASV010000061        21322 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYASV010000061 DAYASV010000000
VERSION     DAYASV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.061"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            250..771
                     /locus_tag="ACGI68_002168"
     CDS             250..771
                     /locus_tag="ACGI68_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138603.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            805..1581
                     /locus_tag="ACGI68_002169"
     CDS             805..1581
                     /locus_tag="ACGI68_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFH0138604.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDKI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1923..2876
                     /gene="gpr"
                     /locus_tag="ACGI68_002170"
     CDS             1923..2876
                     /gene="gpr"
                     /locus_tag="ACGI68_002170"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HFH0138605.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2894..3913
                     /gene="spoIIP"
                     /locus_tag="ACGI68_002171"
     CDS             2894..3913
                     /gene="spoIIP"
                     /locus_tag="ACGI68_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HFH0138606.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3944..4195
                     /locus_tag="ACGI68_002172"
     CDS             3944..4195
                     /locus_tag="ACGI68_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138607.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4477..6282
                     /gene="lepA"
                     /locus_tag="ACGI68_002173"
     CDS             4477..6282
                     /gene="lepA"
                     /locus_tag="ACGI68_002173"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFH0138608.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6431..7768
                     /locus_tag="ACGI68_002174"
     CDS             6431..7768
                     /locus_tag="ACGI68_002174"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFH0138609.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8001..9314
                     /locus_tag="ACGI68_002175"
     CDS             8001..9314
                     /locus_tag="ACGI68_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0138610.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9486..10661
                     /gene="hemW"
                     /locus_tag="ACGI68_002176"
     CDS             9486..10661
                     /gene="hemW"
                     /locus_tag="ACGI68_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFH0138611.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10764..11783
                     /gene="hrcA"
                     /locus_tag="ACGI68_002177"
     CDS             10764..11783
                     /gene="hrcA"
                     /locus_tag="ACGI68_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFH0138612.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11813..12433
                     /gene="grpE"
                     /locus_tag="ACGI68_002178"
     CDS             11813..12433
                     /gene="grpE"
                     /locus_tag="ACGI68_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFH0138613.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12525..14372
                     /gene="dnaK"
                     /locus_tag="ACGI68_002179"
     CDS             12525..14372
                     /gene="dnaK"
                     /locus_tag="ACGI68_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFH0138614.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14583..15737
                     /gene="dnaJ"
                     /locus_tag="ACGI68_002180"
     CDS             14583..15737
                     /gene="dnaJ"
                     /locus_tag="ACGI68_002180"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFH0138615.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16703..17638
                     /locus_tag="ACGI68_002181"
     CDS             16703..17638
                     /locus_tag="ACGI68_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0138616.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17674..19047
                     /locus_tag="ACGI68_002182"
     CDS             17674..19047
                     /locus_tag="ACGI68_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0138617.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19235..20002)
                     /locus_tag="ACGI68_002183"
     CDS             complement(19235..20002)
                     /locus_tag="ACGI68_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HFH0138618.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDFI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20032..21141)
                     /locus_tag="ACGI68_002184"
     CDS             complement(20032..21141)
                     /locus_tag="ACGI68_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138619.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNNIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRNLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8348 a         2391 c         3750 g         6833 t
ORIGIN      
        1 taatgaaaaa ttataaaaat ataaatttat atgcttaatt aagataattt ttttcattag
       61 tatgtatgat agatgcatca aacaatataa ctaatatgtt agaaggttat atgtaattaa
      121 tgtcagtgtt tatatagaat agcatttgta gcatttatta tagttgtaat tactatttat
      181 atcatagact tgaatagtct aaaaaaatag actataatat ttttatagac taaagaaagg
      241 gctgataaga tggacatagc aaaactaatt ttaaatcctg caaggcttcg tattttacaa
      301 tatattcgac tttatggcag tgttcgtaca tctgatattg taaagtattt aaatgatatt
      361 cctcgtgcaa cagtttatca ccatgtaaaa attttagaag agaacaatat gattgaagta
      421 attaaagaaa atcgtgtgcg tggtactatt gaaaaggttt atgcactaaa ggaatataca
      481 acatctatgg aaggagagac cttcgttgca ctttctacag cattttatgt aggtctgatg
      541 caggaaatga atgaatactt tagcagaaaa gagcaagacc ataaaaagga taatgtattt
      601 ttctcttcag cattactata tatgagtgat aatgaatatg aaaatcttct taaaagtata
      661 gctgatttat tgaaaccata tatagaacaa aaacctaaat caaatctaaa acttagaaaa
      721 ctttcaatta tatcttctcc acctgtagct gataatgata aaacaaaata acatgcaacc
      781 catttttata aaaggaggtt atatatgaaa tttcatgagt ttggaaataa aaacagacca
      841 catattatgt tgattcatgg gggagggaat gcatggtgga actatttgcg acaagcagaa
      901 gttttgtcta acaaatatca tgtgatactt cctacattgg atggtcatgg agaagaatat
      961 gaaacaacat acatttctac agaggataca gcagataagt tgatatcata tatagatgaa
     1021 aattgcagtg gacatctttt cgctttgtgt ggggtatcac ttggtggtca aattgtcatg
     1081 gaacttttat ctcgaaaacc taatattact aaaaaagcaa ttattgatgg tagtatttgc
     1141 tacccaagac ctcttatggc aaggttttgt attgctggta ttcgattttt tggaggactt
     1201 ttgtttagtg agaaggcttg tcgctttcaa attgctatga tgccaaaatt gttacctgaa
     1261 aatatgcaat atccagataa aataaaagcg tattatatga cagatatgcc acatgtaaga
     1321 aaagaaacat tctataatat gtatcgtact tacatgatga actatacact taaagagaat
     1381 gttaaaaaaa ccacagcaca agtaatgtac tggtatggag aaaaagaaat gaagtgtgtc
     1441 aaaaaatctg caaaactgtt tcagtcttat gtgccctctt gtgagattta tgaagctaag
     1501 agctataatc atgggtattt atcactttat cttccaaatg aatggttgga aattgcagaa
     1561 ccatttttcc aaaaggaata aaggtgttag gtgattattg tataattatt gctgtttttt
     1621 tatatagtaa ataaattagg tataaatata aaaaattatt ttagttaata tattagtgaa
     1681 agcttaatag gaaaattata aatagattaa aggtagcacc tatataaata gttgctacct
     1741 ttttctgttt gtaaataaat gatagataag aaggtttact gacaaatcac tgactaaacc
     1801 caattttctt acattgaaag ccttaatttt acttgtttta aataaattta caatttcttg
     1861 ttataaaata ctaaattttg ttaacaatta taattagcat ttaatttata ctggaggttt
     1921 atatgattag tgtaagaaca gatttagctt tagaagctag tgaaatgtgt gaaaagtctc
     1981 aagaaggaag tagtattcct ggtgtaaaaa tagaaactaa agaattagaa aattgcattg
     2041 taactaaagt tgaagtaata gacaggcaag gcgcagagat aatgaataaa gatataggaa
     2101 agtatataac tctggaaagt aatcttatga agtttgatga tgatgagtca agagaagaaa
     2161 tgataagtta tttaaaagag gagttagtag atatatttgg ccaagacaaa aataaaaaaa
     2221 ccctggtaat tggtcttgga aatagaaata ttacttctga tgcgttagga ccaaagagtg
     2281 tatcaaaaac acttgtaact agacatcttt ttaaaaatta taataaagac tatgatgatg
     2341 atttcactga ggtatcagct ttaagcccag gagttatggg tgttactgga atagaaacta
     2401 gtgaaatagt aaaatcattg gttgagaagg ttaagccaga tagagtagta gcaatagatg
     2461 cattggcttc aagaaaaatg gaaagagtaa actctactat acagatatca acagcaggaa
     2521 tatcacctgg tggaggtgtt ggcaatacta gaaaatctct aactaaagaa actttaggcg
     2581 tggatgttat tgcaataggc gttccaacgg tggttgatgc agcaactctt acaatagatg
     2641 tcttagatat ggcaatagac aatttaatag ctcaatcaga agaaacagaa agtttttatg
     2701 aaatgttgaa aaaacttaaa gaagaagaaa aatatcatct aataaaagat tcgttagacc
     2761 catatgacaa gaacttaata gtaacaccaa aggatataga tgatacaatc gaaaatctat
     2821 ctataataat aagtgaaggc ttaaataggt ctttacatcc aggtagattg gtataaaata
     2881 aggaggagca attatgttta aaaaatgcat taaagtagta accttgactt ttatattagt
     2941 atgcatttta ccaggaaaat cattagcttt gaatcaagat gattttttaa agtttttagt
     3001 aaattcatct tatccagaag ctaaagtcga aggaaatgat acagaaaata aaaagaataa
     3061 taaaaataaa gaaacaagta aggaaaataa ggaagaaagt aaagaaggaa acactaaatc
     3121 taaagatgca agtaaagttg ataataaaaa agagagtgaa aaagaatata taaaacttta
     3181 tgttggtaaa gaaaatgttc cagatattga gtcaaaaaat tcagacacaa ctgaaactaa
     3241 cacaacatct agttcagatt ataaggatga tttaagagta accaaagaaa atccaagaat
     3301 tttgatatat catactcatg gatgtgagac ttattcaaat tcacctgatg gaaattatca
     3361 ttcaagagat aagaaaaatt cagtaatgga agtaggaagt gcattaacta gtgctttaga
     3421 tagcaaagga tggggtgtag tccatactac taaataccat gactatccat cttataataa
     3481 ctcttatgca agtagcttaa aaacaataca aagtatatta cctaaatata actcagtgga
     3541 tatagcgata gacttacata gagatgctag ggatttaact aatccagcca ctaaggaaaa
     3601 agaccattta aaatatacaa ctatgataaa tggagagaga gtatcaaagt tcttctttgt
     3661 tgtaggaggt aaaaatacaa atagaaaaca acttagagct ttagcagaag atataactgc
     3721 ttttgctgag aaaaagtatc ctggtctagt atctccaata gtagaaaaag actatgctag
     3781 attcaatcag tttgcagtta agaatcatat gttagtagaa atagggaata acgcaacaag
     3841 tgtagaagaa tctaaggcta ctacaaagta tttagccgag attttggatg aatattttaa
     3901 acaaaaaaat tagagcttta agttataatt aggagcatgc taaatgagcc gattagaaaa
     3961 aaatatagaa agtaaaagta aactaagaaa atacaagtat attatgagaa tcttattttt
     4021 gattactatg attatatgta catcagtttg tgtatttata gtggacagta atgcaaaaag
     4081 tatgctagga atgaaacaat atgattttcc aagtattaaa tttgatataa caaaatttga
     4141 ttttgacata ccaaattttg atatacatga aatcataaaa aaatttaata agtagtaata
     4201 agtgaataag gtttattgat taaaataagc cttattttag ttatatattt ttaatgtata
     4261 aaataaaaat tcattaactg tattttatag gatttacttt aagtttatga tattatataa
     4321 tatttagtgt tagacttgat aatccacata taatgtggat aattttgttt agtgccaatt
     4381 agtttatatt caaaaatttt aaatttggta tataaagagt ttttagataa tgtaaaataa
     4441 taactataag aataatcaag aggagggaat tttaaggtgg acaataaaca aagtagaact
     4501 agaaatttta gtataattgc acatatagac catggaaagt ctaccttagc tgatagatta
     4561 atacaacaaa ctgggcttgt tagtgaaaga gacatgaaaa gtcaattact tgacaatatg
     4621 gaccttgaaa gagaaagagg aatcactata aagcttcaaa acataagatt aatgtataaa
     4681 gcgaaggatg gaaatgaata ttatttaaat cttatagata cacctggaca tgtagacttt
     4741 aactatgagg tatcaagaag tttagctgca tgtgaaggag cactattagt agttgatgca
     4801 gctcaaggtg ttgaggcaca aactttagcc aatgtttatc ttgctataga ccaagattta
     4861 gaaatattac caataataaa taaaatagat ttaccaagtg caagaccaga ggaagtaaaa
     4921 aatgaaatag aagatttaat agggcttgat tcaagtgaag ctcctcttat ttcagctaaa
     4981 actggtttaa acatagaaga tgttttagaa gatattgtaa aaaatgttcc tccacctaaa
     5041 ggagataatg aagctccatt aaaggcatta atattcgatt cttactatga tgcttacaaa
     5101 ggtgttgtag cttatgttag agtatttgaa ggaactgtta aaaagggtat gacaataaaa
     5161 atgatgaata ccaataaaaa gtttgaggta actgaagttg gtgttatggc tccaggtcaa
     5221 actgaactta gtgaattatc agcaggtgat gttggttata ttgctgctag tataaaagat
     5281 ataagaagtt gtcgtgttgg tgatacaata acagattcaa ataatcctac agaagaacct
     5341 ttaccaggat acaaaaaagc tactccaatg gtatattgtg gtatatatcc aggagaagga
     5401 gaaaagtatg agaatgttag agatgctctt gaaaagcttc aagtaaatga tgctgcactt
     5461 gaatatgaag ctgagacttc agcagcacta ggatttggtt ttagatgtgg tttcttaggt
     5521 cttttacata tggagataat gcaagaaaga cttgagagag aatttaacct tgatataatt
     5581 actacagctc catctgttat atatagagtt acaaaaatgg atggagaggt tgtaatgata
     5641 caaaatcctg caaacttacc agaaccatct gagataaaga tgatagaaga gcctatagta
     5701 aaaggagata taatagttcc taaagactat gtgggtgttg ttatggagct ttgccaggaa
     5761 agacgtggaa atatgttaaa tatggaatat atagatgata gaagagtaat gcttcactat
     5821 gaccttcctc tcaatgaagt tgtttacgat ttctttgatg ctttaaaatc aagaactaga
     5881 ggttatggtt ctcttgatta tgaggttaaa ggttatgtag catctacact tgtaaaactt
     5941 gatatattaa tcaataaaga gcaagtggat gcacttagtt ttattgtcca tgagacacgt
     6001 gctttcccta gaggaaaggc aatgtgtgag aaattaaaag gagaaattcc aagacatcag
     6061 tttgctatac caatacaagc tgctgttggt aataaggtta ttgcaagaga aacaataagc
     6121 gctctaagaa aagatgttct tgctaaatgt tatggtggag atatatctcg taagaagaaa
     6181 cttcttgaaa aacaaaaaga aggtaagaag cgtatgagac aaattggttc tgttgaagtt
     6241 cctcaaaaag catttatgtc tgtgcttaaa ttagatgagt aaatccttat aaatttagat
     6301 aaagatattt aaaagctaga gtgattttag aattatttct aaattagtct agctttttta
     6361 tggtatattt taagtaaact catagagaat ataagtaaga atattaaaga agaataaaaa
     6421 ggtgatattt atgattaata tagaaattcc taaaaaagta gactatataa ttaaagaatt
     6481 agaaaagaat ggttatgaag cttatatagt tggaggatgt gttagagatt gtttattaga
     6541 aagagttcca aacgattggg atataacaac tagtgctaga ccagaagtgg tagtagaatt
     6601 atttgaaaaa acaattccaa ctggtataca acatgggact gtaacagtta tgattgaaca
     6661 tgaaccattt gaagtgacga cttatagaat tgatggaaat tatagtgatg gtagacatcc
     6721 agattcaata gaatttacaa ataacatagt taaggacttg tcaagaagag attttactat
     6781 caattcaatt gcatacaatt caaagacagg attagtcgac ccatttaatg gctatgaaga
     6841 tatacaaaat aagtatatac gttgtgttgg aaatccagta gatagatttg aagaagatgc
     6901 acttagaatg cttagagcag ttagattttc agcacaactc aattttaaaa tagctgaagg
     6961 tactaagcaa agtattcata aaaaagcaga tttgataaaa aatgtttcta ttgaaagaat
     7021 ccagacagag tttaataaaa tattagtatc tgattcatct aaattaaact tattaaaatc
     7081 aacaggtttg ttgaaattta taattcctga aatatgtgaa ttagaagatg ttactcaaca
     7141 caatccatat cacatctatg atgtacaaaa acatacttta atagctacag aagtaattga
     7201 agatgaactg tatttaaaac ttacaatgtt gtttcatgat ttaggtaaaa aagtaactaa
     7261 aacaactgat aaaaatggtg ttgaccattt ttatactcat agtagagaat ctgtaaagat
     7321 tgcaaagaag attttgaaaa gacttaagta tgataattat actattaata aagttttaat
     7381 attaatacaa tatcatgatt atagaataga acctaaaagg aagataatta aaaagttatt
     7441 aaataaactt gaagatgttg aattatttga agatttaata aaagttaatt gggctgatac
     7501 acttgcaaaa aatccaaagt atgcaaaaca aaagatatta aatcttattg agtgtgaaaa
     7561 agaatttaaa catataataa atcaaaagga gtgctttaat cttaaagatt tagctattaa
     7621 tggaaaagat ttgatatcta taggtataaa accaggtaaa gatataggat atattttaaa
     7681 taagatgctt gaaatagtca taaataatcc agagttaaat gaaaaagaaa tactaaaaga
     7741 aaaagcatta aatatatata cattttagat tcaaatataa aaaatttaat tactttaaaa
     7801 aacatagaat aaaatactta tttagataaa ataaataatt tttagagaat attttagaag
     7861 ttacgattgt atgattttta aatgcatagg catttactct aggactatag actaatataa
     7921 aaaaaaactt tgggaatact aaaaccataa atttataaaa ttaatatttt atatactgta
     7981 aaacagtgag gagggacagt atgcatactc aactacaaaa gactattggt ctttcagcag
     8041 ctctttctac tgttgtaggt atggttatag gttcgggagt tttttttaag ccacaagcaa
     8101 tatacactac aactaatgga gctccaggtc taggtattat agcgtggctt ttaggtggtt
     8161 ttataacaat aactgctggg cttacagcta cagaaatttc agctgctata ccaaaaactg
     8221 gtggtatgat gatttatatc gaagaaatat atggagaaaa gttaggtttt ttaactggat
     8281 ggatgcaaac tgtattattt ttcccaggta catcagcggc attaggagta atatttgctc
     8341 aacaagcatc tgaactttta ggaatgagcc caaataatat ggcaaatgta cttcctatag
     8401 caattggagt tatactattt ttagcgttat taaatataat aggttcatcc ttaggaggaa
     8461 aagttcagac agtagccact attggaaaga tgataccttt aattttaata atagtctttg
     8521 gatttattaa aggtcaaagt agtgaagtat taaatccatt tgtaggagat ggtgtaaatg
     8581 catcaaatgc acttggacaa gtattaatag ctactttgtt tgcatatgat ggttggataa
     8641 atgttggagc tatatcagga gaaatgaagt ctccagagaa agatttacca agagcaatag
     8701 ttggtggttt atcattagtt atggcagttt atattataat aaatatagct tatctttggg
     8761 tagtaccagc aagtgaatta gcaacagtta cttccccagc tacattagta gcaacaagat
     8821 tgtttggaaa tataggtgga aaagtaataa ctgtgggtat attaatatca gtatttggga
     8881 cattgaatgg atatttatta acaggttcaa ggatacctta tacattagct gaaatgggaa
     8941 ctttaccagc atctaaaact cttttaaaag taaattctgg aggttctcct gtaaattcaa
     9001 tactacttat aactgtgtta gcctgtgtat atgcattatc tggtcaattt aatcttttga
     9061 cagacttaac aatattttca atatgggtat tttatgtatt gacttttata ggtgttatga
     9121 ggcttagaag acaaaagcca gatttacaca gaccatataa agtcccttta tatccaataa
     9181 taccaattat agctatatta ggtggactgt ttgtaataat aaatcaaata ttgacttcaa
     9241 cagtcatatc tttaggcgga atatttataa cactattagg tttaccagtt tattattata
     9301 tgaaaaaaag ataactactt aatttaagtt agataaattt gggtaaaggt gaaaattaac
     9361 ttttatccaa attttttata ctgttataca atataagtat tatatattgc ttgatgtgct
     9421 attttgtttg atttataatt tagtaattga tgttttaaga taatttaaga aatgaggagt
     9481 gaaagatgct aggactttat gtacatattc cattttgtgt gaaaaaatgt aagtattgtg
     9541 attttaattc ttataaaatg gacatagatt caaaaaaaag atatatagaa gacttaaaaa
     9601 tagaaatgga attatatagt aataaattat ataaagataa taaatataaa aataaagaat
     9661 gctgtagtct aaataaaaat gacaaaataa cgagtatatt tgtaggggga ggtacaccta
     9721 gtatactaac ctctgatgaa ataagagaag tatttataag tataaaagaa atgtttgata
     9781 tagatgagaa tgcagaaata actatagagt gtaatcctgg gacattaaca ttagaaaaat
     9841 taaaaactat gaaagagatt ggcataaata gacttagtat agggctacaa gctattcaag
     9901 aaaagcattt gaactttata ggaagaatac atacttatga agaatttgag aaaaactata
     9961 aagatgcatt aagtgtaggt tttaaaaata ttaatataga tttaatgtac agtcttccaa
    10021 atcaaactct atgtgattgg aaggaaactc ttgaaaaagt tgtacactta aatccaacac
    10081 atatatcagc ttattccctt atactagaag aaggaactga actatacaat atgtatgaaa
    10141 gtaataaatt tgaacttata gatgaaaatg tagatataga aatgtatgag tatacaataa
    10201 actatttaaa gtcaaaagga tataatcaat atgaaatatc aaactactca aaagaaggct
    10261 ataattgtga gcataatatt ttatattggg aatgtgaaca ttatataggg attggagcag
    10321 gagcttctgg ctatattaat gagaacagat acaataatgt agaatcttta gaagattatc
    10381 atttaagttt ggtaaaaaga gaaaaaccaa tacaagaaaa tgaaattctt tctgagaaag
    10441 atatgataga agaaaagata tttatggggc ttagaatgaa taaaggcata aaatttgaag
    10501 attttaaaaa aaaatttgga atagatttta gagagaagta taataagcaa atagaaatgc
    10561 tattagctag aaagcttata aatcagagct ttgaaggtat acaattaact caaaaaggaa
    10621 gagaaatatc taatagtgta tttatagaat ttatggaata atttttaagg tgttgacaaa
    10681 ataaatttat aatggtatat aatatatata atctttttag cactcgaact tagtgagtgc
    10741 taacagctag gaggtataat aatatggaat taaatgaaag aaaattaaat atcctcaaag
    10801 ctatagttaa agattatata gaaacagctg aagctatagg ttctagaaca atatctaaaa
    10861 gacatgattt aggtgttagt gctgctacca taagaaatga aatggctgac cttgaagaat
    10921 tagggtattt aattcagcct catacttctg cgggtagagt tccatctgaa aaaggctata
    10981 agttatatgt aaactctctt atgagtaaaa gcgaattgga tgataatgat aagattctta
    11041 ttgaacaatg tatgaatcat aatataaatc atataaaaga attgattcat gaaacttcta
    11101 agctattatc gcaactaaca aactatacaa ctgtagcagt tactaagagt ttaataaatc
    11161 aaagtgttat aaagcacata cagttggttg ctatgaatga caataatatt ttacttatag
    11221 ttgtaacaga taaaggggac ttaaaaaaag ctaaccttac aactaatgta tatttagatc
    11281 aatctaagtt aaatttgatt tctgataatc ttacaaggaa gctcttaggt aaaagtataa
    11341 cagatttgga tgataatcta attgctttta ttaaatacga aataagtgag tattctgggc
    11401 ttatagatga gcttttaaat gctttgaact caaatatgaa agaagaagat ttttctctat
    11461 cattaaatgg agctactaac atatttagtt atccagaatt taatgatgta ttaaaagcaa
    11521 agtcattttt aaatatgttg gaaaaaaagg aaacaatagc tgacataatt aaatcaaagg
    11581 gaatacaaaa agacaatcta aatataataa ttggtagtga taatgattgt gaactggcac
    11641 aggattgtag tatagttaca gcaacttata atgtagatag agacttggtt gggcgaataa
    11701 gctttatagg ccctaccaga atggattatg ctagaattta ctctataata aattatatga
    11761 gtttattaat taatagaaag taaagaaatt ttaaattgcg aggtgtaaag tcttggataa
    11821 aaaaaatgag caacaggaag taagagaaga aaatgatact tctattaacc aagaatcgga
    11881 aactcaagta gagttagaag aggaagttgt taatgaagaa tgtgaaacat caagtgaaaa
    11941 aactgatgaa aaagaagttg atgatgaaaa tgtaacagat ataaattcta agttagcaga
    12001 aaaaaaattg caggatgagt tggatgaatt aaatgataaa tatcaaagac ttcaagccga
    12061 atatgcaaac tacagaagaa gaactcaaca agagaaagag acaataggcg tatttgcaaa
    12121 tgaaaaaata ataactgaat tgataccagt tatagatagt atggaaagag ccttagatgc
    12181 atgtgaagat aaggaagata ctatgtataa gggtataagc cttgtacata aacaattaat
    12241 agacacttta gtaaaatttg gagtagaaga aatagaagca gaatctaagg aatttgaccc
    12301 taatctacat ctagcagtta tgcaagaaag tgtagatgga gttgaagcaa atcaaatagt
    12361 aatggtactt caaaaaggat ataagctagg aactaaagtt gtaagacctt caatggttaa
    12421 agtttcttgc taagtataaa ttaaaggtta aattatttaa cctaatataa aatattaaaa
    12481 taagtaaaac ttaattatat aattactcga ggaggatttt agtcatggga aaaataatag
    12541 gaatagattt aggaacaaca aattcatgtg ttgcagtgtt agaaggtgga gaagcacaaa
    12601 taatagcaaa tagtgaaggt atgagaacta ctccatcagt agtagcattt acaaaagatg
    12661 gtgaaagaat agttggagaa cctgcaaaaa gacaagcagt tacaaatgca gataaaacaa
    12721 taacttctat aaaaactcat atgggaactg attataaagt aaatatagat ggaaaatcat
    12781 atactccaca agaaatatca gcaataatat tacaaaaatt aaaatcagat gcagaaagtt
    12841 acttaggaca aacagtaaca gaagcagtta taacagtacc agcttacttt acagatgctc
    12901 aaagacaagc aactaaagat gctggtagaa tagcagggct tgatgttaag agaataataa
    12961 atgagccaac agcagcagct cttgcttatg gtatggataa attagaccaa gaaaagaaaa
    13021 tattagtatt tgacttaggt ggaggaactt ttgacgtatc tatacttgaa ataggagatg
    13081 gaacttttga agttttagct acagctggta acaatagatt aggtggagat gactttgacc
    13141 aaatagtaat agattattta gcagaggaat ttaaaaaagc tgaaggtgta gatttaagaa
    13201 atgataaaat ggctcttcaa agattaaaag aagcagcaga aaaggctaag aaagaattat
    13261 catcaacaat gagttcaaac ataaacttac ctttcataac tgctacagca gaaggaccaa
    13321 aacacttaaa tatagattta tcaagagcta aatttgaaga attaactaga ggtttagttg
    13381 aaaaaactat ggaaccaact aagacagctt tacaagatgc aggactttct acaggtgata
    13441 tagatgatgt attattagta ggtggttcta caagaatacc agcagtacaa gaagctgtta
    13501 agaagtttat aggaaaagaa cctcataaag gtataaaccc agatgagtgt gttgcagcag
    13561 gtgcttctat acaagctgga gttttagcag gggatgttaa agacttatta ttactagatg
    13621 ttactccatt atctcttggt atagaaacta tgggtaatgt aatgactaag ataatagaaa
    13681 gaaatactac aataccaact aagaagtctc aaatattctc aactgctgct gataaccaaa
    13741 cagctgttga tatacatgtt cttcaaggtg aaagaagtat ggcttatgac aatacaactt
    13801 taggaagatt ccaattaaca gatataccac cagcacaaag aggaatacct caaatagaag
    13861 ttacttttga tatagatgca aatggtatag ttaatgtttc agctaaagat ttaggtacag
    13921 gaaaagagca aaaaataact ataacatcaa acactaattt atcagaagca gaaatagagc
    13981 aaaagataaa agaagcagaa atgaatgcag aagctgacaa acaaaagaaa gaaaaaatag
    14041 aagcattcaa tcaagctgaa tctactatat atcaaactga aaaaacttta aatgaacttg
    14101 gagataaaat aagttcaggt gaaaaagagg atatagaaaa agctatagca gacttaaaag
    14161 ctgtaaaaga taatcaagat gctacagcag aagaacttaa gaaagctaca gatgaagtaa
    14221 tgactaagtt ccaaaaagta tctcaagaaa tgtaccaaaa agcagctcaa gaacaacaag
    14281 cggcacaagg tgcagaacaa gcacaagaca atggacctaa agatgacaat gtagtagatg
    14341 ctgactttaa agaagtagat gaagataaat aagctatata atatgtaaca ctaaatgata
    14401 attggaacat atatgatttg tgaaaatcat atagatgtta aagatatatg gacaatatac
    14461 atcataaaga atataatata tcatagatta tataacttaa agtagaatat aagaattaat
    14521 gatagcttgt agtttattat tctacaagct atcgttttgt aaaatgaagg tggtgtcaat
    14581 ttttgagtac aaaaagggat tattatgaag tcttaggtat cagtaaaggt gcagaagctc
    14641 aagagataaa aaaagcttat agaaaattag caatgaagta ccatccagat agaaatcctg
    14701 gagataaaga ggcggaagaa aaatttaaag agataaatga agcttatgaa gttctatctg
    14761 atgatacaaa gagaaaaact tatgaccagt ttggacatga tgggctaaat ggtcaaggcg
    14821 gttttggtgg tcaaggagga tttggcggtc aaggctttgg tggttttgaa gatatgttcg
    14881 gagatatatt tggtgacatg tttggaggaa gcttcggagg tggaagagca agaagaagag
    14941 ggcctcaacg tggtgcagat ataagacaga gtgttactat atcttttgaa gaagctgcat
    15001 ttggtaaaaa gatgtctata aaagtaaata gaagtgaaga atgtgaagaa tgtaatggaa
    15061 ctggagcaaa accaggaaca tcaaagaaaa cttgttcaac ttgtaatgga actggtcaag
    15121 ttagaactgt tcaaagaact ccatttggta atatagcaag ttcaagacca tgtagtgctt
    15181 gtaatggaac tggagaagtg attgaatctc catgtagtaa atgtcatgga actggaaata
    15241 caagaaaagt taagactata gaagtagata tacctgctgg tatagatgat ggtcaaatga
    15301 taaaactttc tggtcaaggt gaagtaggag aaaaaggtgc tccaagagga gacttatata
    15361 ttgtagttaa tgttaagtca catccactat ttactagaga tggaaatgat atttattttg
    15421 aaatgccaat tacatttgtt caagcaactt taggtgatga gatagaagta cctactttgg
    15481 atggaaaagt taaatatagt gtgccagagg ggacacaaac tggaactgta tttagactta
    15541 aagagaaagg aatccctaga attagaggta attcaagagg agaccaatat gttaaggttg
    15601 tagttgaaat tcctaaaaaa ttaaacgata agcaaaaaga actattgaga gaatttgcga
    15661 aggagtgtgg gtcgaacgtc cacgaaaaga agaaaacctt cggacaaaag atagaagata
    15721 tgtttaagaa aaagtagtta aagtagccaa tatataatca tatttttaaa atcttagtta
    15781 tttataagat tgtcaaagga gatattatga tttttatatt ggctattttt gtataaaaaa
    15841 taaatgtgca taaaataaaa ctgaatataa ataattttct gaaaaatact tgtcctaaaa
    15901 tatataatat gctataatat aatcaaataa aagaaagggg tgattccagt gggaacggat
    15961 gtggcactgc caaatgatga agaaaacttt attgtaatat acaaaataaa attagatact
    16021 tttagtgcta gattaagtac tggaaaaact catgagctgg gataattatt gtaattacct
    16081 agactcaaac ttttagtagt aaaatagtaa tattatatag acaaggtatc taatttttaa
    16141 atcttttgta tgaattaaaa tttttataaa gggtatatct gattagaatg taattaggga
    16201 aacctagttg tgaattactt ttagtatata catgtgtaaa gaaatatgta tatggtattg
    16261 gttattgtgc tagatgtgta tgtaaaggaa tatgtgtcta gttgggtata tgtaaagaaa
    16321 tatacttata atctatgaaa aatttttagt ataaattgct aaaacattga gattgttttt
    16381 tatagaaagt tagtttatac caactaaaaa tgtgcttaag atttgtttga tgtttaatta
    16441 taaataaggt tgtagataaa aaaataagta aagacaaagg tttaaaatac ttgctttaaa
    16501 tatagtttgt tttgaacact tgccaaatta aaataattaa taaaatagac atcttctcat
    16561 agtaaaatat actgtgagag gatgtttttg ctttgttcat aaacttttat gaaaaatttg
    16621 catagtgtca cattttaata tactatatat ataataaaat atagataaaa ttaaatgatt
    16681 caaaagagaa aggtgaaaag agatgtatta tataggtgta gacataggag gaacaggtat
    16741 acaagcagga gtagttgata actatgggaa aataattttt aggtcagagt gtaaaacagt
    16801 aattgaaaaa ggctttgaag gaatacttaa tgacataaaa ataatgattt ataagttgtt
    16861 ggaagataat aaacttacta tgagtgacat aaaatctatt ggatttggtg ttccaggttt
    16921 tataaataaa gaaggattag taacttgtgt aaatttaaaa tggaataaaa aagcttttaa
    16981 taaagagtta aaaagaagat ttccagatgt tgaaattcat ggagaaaatg atgcaacagt
    17041 agcagcactt ggagaagcaa agtttggaag tatgaaaggt gctaatgttg gagtattata
    17101 tactttagga actggagtag gtggaggaat agtaattaat caaaaagtct tttctggagc
    17161 acatggattg ggttcagaaa taggtcatca aattatagga gaaaactatt ttaattgtaa
    17221 ttgtggaaat aatggttgtg tagaaacttt ttgttcagca actgcaatta taaagtattc
    17281 tcaaaaactg atagaagaag gagaaaagag taggatttta gatttggcag agggaaattt
    17341 agaaaatgta aatgcaaaga tggtgtttga tgcatataga gaaaatgatt tagtggcaat
    17401 taaagtaata aatagattta aagagtattt agcaaaaact tttgcaaaca caataaattc
    17461 tttagaccca gaaattatat ctataggagg aggcatttct aaatctagcg atataatact
    17521 tgatggaata gaagatttgg taagaaaatt tgtattatac aaaacagaag atatagctac
    17581 aattacatgt gcaactttag gttcagacgc aggaattata ggagcagctt ttttataatg
    17641 taaatgaact gcttattgga ggcgtgatga tatatgggta aaacaaatgc tagaagaacg
    17701 ctttttttga tagcaatagg ttcaggaact atgctgaatc ctcttaattc ttctatgatt
    17761 tcacttgctt tacatagtat acaaaatgat tttcatatta gttttacaac tgtatcttgg
    17821 attgtttcag cattttacct agctagtgct attgcacaac cagtttcagg taagatagga
    17881 gatttaatag gtagaaaggt cttatttttg agtggtttga tgcttgtaat tttatcagca
    17941 tccatagtac cacttgttca atcattcttt atattgattt ttataagagt aattcaagca
    18001 attggaacaa gtaccctata tccatctgga gttgctctta tacaaaataa tataaaagaa
    18061 agacaatctt ctgcattagc agttctaacc atttttgctt caactacagc aggattaggt
    18121 ccgacacttg gaggattatt gttggactta ggaggatggc atgctatatt tcttgtaaat
    18181 atacctgttg ttttggtttc tatatgctta gggtattttt tattcccaaa agaagtaaaa
    18241 gaaaagaaat ctataaaaga aactttaaaa aatgttttgt caagattaga tattattggc
    18301 atacttctat tttcaattgc aatgattttt atattactat ttttgctatc tatgaaggag
    18361 tcttttaact tggaacaact tgtatttggt ataatactga tgtgtctttt tatttggcat
    18421 gaattgagaa caaaattgcc atttatagat attaagcttt ttgtaagtaa tcctaaattg
    18481 tcaaaagttt acttgcaatt tattatatta aatttcttta attatatttt gttttttggt
    18541 cttcctagtt attttcaaga tgccttacat tatagtgcaa agtcgactgg attatttatg
    18601 ctatttatgt ctggtatagg tatatttata tctccattaa ctggaaaatg gatagataaa
    18661 tctggaacta gatttcctct tgttacatca tcaatcttta tgtttattag tgcagtactt
    18721 ctttcagtag tttttatcca tatacctgta ataggaaagg gtattatatt atcattagca
    18781 ggtattagtt atggagttgg taatgtagcc ctgcaatcct ctatgtttga agaaagtcct
    18841 cgtgacagta tagggacagc ttctggtctg tttcaaacta gtcgttattt aggttctatt
    18901 ttagcaactg ttttacttgg aatggtattt gaagtaaata taacatcaga acaatttcag
    18961 atattaggtt atgttatggt ggtcttaggt acaataagtt ttttattaaa ttttatatta
    19021 aagaaagaat taagaaaagt agaatagtgg atttatggat ttaataagtc taataactga
    19081 taagtttaaa aatattgttt gtaaaacata actggtttga tatttattaa ttctaagttt
    19141 tgaagcatag agtactcgat aagtatacaa tattttgaat taaaattaag cagaaatttg
    19201 aactagtctt tcaaatctct gcttaattta gtagttaaat ttttttatag agaaaataag
    19261 atttgacact tagataaaaa atcattgcat tttcaaaatt ctttaaatca aacccaagta
    19321 tctcattaat cttttccaat ctatatacta gcgtgtttct atgtaaaaac atactagtag
    19381 atgtcttaga aatatttaaa tcattattta taaattttat tcctgtatat agaagttctt
    19441 cgtcaataga tttaatctta ttaaaatcta ttaatttatt gagtatatta tcctcattaa
    19501 ttaaatctaa agttttatat ctaataaagt caaacaaatt taaaaactta ttttcagcgt
    19561 tgtcttttat aatatgaggt agtaaatcag aacataactg taatctaaaa tcaaaatcag
    19621 ctttactatc taattcacca agataaaaaa tataagtact ttgataagtt tcagtttcaa
    19681 ttatattttg tgtagttaca ttaatatcta agttatattt agaatttact ataaaaaaac
    19741 cattgtgttg tattatattt atatcttcaa ataaattata aaaaatattt tttagatatt
    19801 caaagtctat atgagattgg taaattaaat aaaagttgcc ttttaaatta tcgagattat
    19861 ttttatctag aaaatcaata acataaatag cattattgaa agtaactaaa tggctactag
    19921 catctaactt ttggttttca tatggataga tatttatttt tttattaagc tgtctttcta
    19981 aaaaatcttt taactcttcc ataaagtctc cttaaaaata agattatatt cttatcttat
    20041 agctaaagta gtttctttgt caaatatgtg tactctacta ttgtcaactg aaagtgttaa
    20101 gtcatcacca acattaattc tgtttttact gtcaaatcta attgttatcg tattttcgcc
    20161 tagttttaag tgagcataag tttctgctcc cataagttct tttatttcaa ttcttgattt
    20221 aaaacaagta tctggattag atttaataaa agtatcttct acatgaatat cttctggtct
    20281 gatacctata acaacttcat tattaatata attgtctttt attaaatcat agtgtttttc
    20341 tttattcaat ttaagcttaa tatgttcatt ttgtgcataa atttcatcat tttcttctat
    20401 taatattaca tcaataaaat tcatctgtgg tgctccaata aatcctgcaa caaatatatt
    20461 agctggataa tcgtatacat cttgtggagt tgcaatctgc tgaactactc catccttcat
    20521 tactacgatt ctatcagcca tagtcatagc ttcaacttgg tcgtgtgtta catagataaa
    20581 tgtagttcca agctgttggt gtagcttagt tatctctgtt ctcatagcag ttctaagttt
    20641 tgcatctaag ttggatagag gttcatccat taagaatacc ttaggatttc taactattgc
    20701 acgtcctaag gcaacccttt gtctttgtcc tccagataat gttttaggtt ttttatttaa
    20761 aagaggtagt aagtctaata ttttagcagc ttctttaact tttttgtcta tttcatcttt
    20821 aggcaaattt ctaagtttaa gtgcaaatgc catgttttcg taaacagata agtggggata
    20881 taaagcatag ttttgaaaaa ccatagctat atctctatct tttggttcta tattattgac
    20941 aagtttatct ccaatgtata gttctccttc agagatttct tcaagaccag caatcattct
    21001 aagtgtagtc gatttaccac aaccagatgg tcctactaaa acgataaatt ctttatcttt
    21061 aatgtctata tttatatttt ttactgcgtt aaatccattt gagtaaagtt ttgatatatt
    21121 ttttaaaatt acttctgaca taatatcctc cttgatattt aaattttaat tatatagtat
    21181 caaatttttt tattatatta aatgtgtaaa atgactaaat gtatttgttt aatatgtata
    21241 tttagttttt agcaaagaat aaaactaaca gtagaatata tttaaaaaat taaaaaacaa
    21301 aattaaattt attatcaaaa aa
//
LOCUS       DAYASV010000062        21183 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYASV010000062 DAYASV010000000
VERSION     DAYASV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21183
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.062"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            154..1164
                     /locus_tag="ACGI68_002185"
     CDS             154..1164
                     /locus_tag="ACGI68_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0138620.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1300..1788
                     /locus_tag="ACGI68_002186"
     CDS             1300..1788
                     /locus_tag="ACGI68_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0138621.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1964..3892
                     /locus_tag="ACGI68_002187"
     CDS             1964..3892
                     /locus_tag="ACGI68_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0138622.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4069..4479
                     /locus_tag="ACGI68_002188"
     CDS             4069..4479
                     /locus_tag="ACGI68_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFH0138623.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4501..4803
                     /locus_tag="ACGI68_002189"
     CDS             4501..4803
                     /locus_tag="ACGI68_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HFH0138624.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5020..5712)
                     /locus_tag="ACGI68_002190"
     CDS             complement(5020..5712)
                     /locus_tag="ACGI68_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFH0138625.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5738..6925)
                     /locus_tag="ACGI68_002191"
     CDS             complement(5738..6925)
                     /locus_tag="ACGI68_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0138626.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7261..9138
                     /locus_tag="ACGI68_002192"
     CDS             7261..9138
                     /locus_tag="ACGI68_002192"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0138627.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9495..10901
                     /locus_tag="ACGI68_002193"
     CDS             9495..10901
                     /locus_tag="ACGI68_002193"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0138628.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10929..11777
                     /locus_tag="ACGI68_002194"
     CDS             10929..11777
                     /locus_tag="ACGI68_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0138629.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12438..13130
                     /locus_tag="ACGI68_002195"
     CDS             12438..13130
                     /locus_tag="ACGI68_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138630.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13127..14062
                     /locus_tag="ACGI68_002196"
     CDS             13127..14062
                     /locus_tag="ACGI68_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0138631.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            14210..15133
                     /locus_tag="ACGI68_002197"
     CDS             14210..15133
                     /locus_tag="ACGI68_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138632.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15117..15887
                     /locus_tag="ACGI68_002198"
     CDS             15117..15887
                     /locus_tag="ACGI68_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138633.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15897..16679
                     /locus_tag="ACGI68_002199"
     CDS             15897..16679
                     /locus_tag="ACGI68_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138634.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17056..17592
                     /gene="rbr"
                     /locus_tag="ACGI68_002200"
     CDS             17056..17592
                     /gene="rbr"
                     /locus_tag="ACGI68_002200"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0138635.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17624..18013
                     /locus_tag="ACGI68_002201"
     CDS             17624..18013
                     /locus_tag="ACGI68_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFH0138636.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18050..18436
                     /locus_tag="ACGI68_002202"
     CDS             18050..18436
                     /locus_tag="ACGI68_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFH0138637.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18563..20005
                     /locus_tag="ACGI68_002203"
     CDS             18563..20005
                     /locus_tag="ACGI68_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFH0138638.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20302..21117
                     /locus_tag="ACGI68_002204"
     CDS             20302..21117
                     /locus_tag="ACGI68_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0138639.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7817 a         2474 c         3896 g         6996 t
ORIGIN      
        1 tatactggaa ttagagtaat taatttattt tttaacatac aaatgatttt atgaaaaaaa
       61 acaagactca aaaattttta tattctttga tagaataaaa ttgagagtaa atttattgaa
      121 ttttataaaa tttataaggg gtgagtctaa gatatgcatg aagataaaaa tgatatattt
      181 gaatcaatag atactgagga ttttgataat aaaacagaag aagaatgttt agagctagta
      241 agaaaaaatg gattgaattt aatatacata aaaaatcaaa cagaagagat atgtctagag
      301 gctgttagac aaaattgtaa tgcaataaaa tgtgttcaga atcagacaga aaaaatatgt
      361 atagaagcag ttaaacaaga ttggagaatg ttagaatttg tggaagagcc aacagaagaa
      421 atttgcatgt tggctattaa ccaagatgga acagcattaa aatatataga aaatcagaca
      481 gaggagttat gcttaagagc cgttgaaaaa aatggtgcag ctttggagta tgtaaaagaa
      541 caaacagaag aaatatgtat agaagcagtt agaaacagcg aatttggatt ggcaagagtg
      601 aaaaatcaaa cagaaaaaat atgtatggaa gcagttaagc attgtagcta taatctaaaa
      661 tgggtagaaa atcaaacaga agaaatatgt atggaagcag tgagacaaaa tgggcttgat
      721 ttgaaatttg taaaaaatca aacagaaaca atctgtttaa gagcagtaag acaaaatgga
      781 atggcattag aatttgtaaa ggaacagact gttggtatat gcttaaaggc agtaagacaa
      841 tatggaatgg cattaaaatt tgtaaaggaa caaacagaag aaatttgtac agaagctata
      901 aaacaagata aaagagcact tagttttgta aaaggagata aggaaaaata taaggctcta
      961 tatgataata atgaaccatt tgcaaagaga tatgttagaa atgtaataga aaaagaaaac
     1021 agagcattga ttaaaaaagg agaagaaaat gcaaaaatca aaattgcagg aaaactttat
     1081 gatagaggca tgtcttttga agatatatca gatatagttg agattgaaat aagcaagtta
     1141 aaagtgagtt taggtgtttt ttaaagtaat aaatgtaggt gttttttatg ggaaatagtt
     1201 aataaaaaaa taatcgacta gctctttgta ggacagcaaa attagtgata taatttgatg
     1261 tataataaat tatttttatt agataagggg gaattaaagt tgaagattac agtattatac
     1321 caaacaagaa gtggaaacac agaaagggtc gctaagctga tagaagaggg cgctaaaaag
     1381 gtggatggag tagatgtaaa gcttatgaga cttgataata ttgatttaga ttatttaaat
     1441 gaaagtaaag cagtaatatt tggaacacct acttatttag caaatacaac ttgggaagta
     1501 aaaaaatggt ttgatgaaga ttcaaaaaaa gttaatttag ctggaaaatt aggagctgtt
     1561 tttgctacat gtgattatat atgtggaggt ccagatgttg cgatacttac tatagtagga
     1621 catttgatgg ttaaagggat gcttgtgtat tctggaggtg gttctttagg aaagccattt
     1681 atacatttag gacatgtaca ttcaattgaa ggtccagagt tacaagatga gaaggctata
     1741 atatttggtg aaagaattgc tactaaggca aaagaactat ttgcttaatt tctatatgca
     1801 tagtaatgat tagagtaaat ttagggtttt aatggagatt tgaagtaaaa gttataaaat
     1861 aaaaaatttt ccataaaatt actcaagatg aattataata taattataaa taaattatat
     1921 tattaaaata tttaaaatca aacgtcaata gcgagggaag tttatggtat ggcatataga
     1981 atatgagatt ttttctgcaa ttatagttat ttttttaatg gtatactttt ttagaagtaa
     2041 atttatacct actttacaaa acaaaatata ttgtgcactt ttaatattct catttttatt
     2101 tataatttca aatattttag gttcattttg tctaaataat atagacaaaa ttccaatttt
     2161 tattactttt ttattgaatc agatatactt attgttattg ccaataccag cagcacttgt
     2221 atcattttat gttatggcta ttatttatca agatataagg tatatgaaaa aaagattatt
     2281 atttttatct ataccactta taatttgtat aatcttatct attacaaatc cttttacgca
     2341 tattctattt ggattgtcaa aagaaacagg ttatatacga ggttgtggat atgttgcaac
     2401 ttttgcatct tttttctttt atgcaatata ttcagcattt ttatctttta aatatagaaa
     2461 agcaatgcat gaatcaaaaa tatgggctat tagaggtttt cttatagttt caattgtggc
     2521 aattatctta caggcaattt ttttacagta tcttcttaca ggaactgcat gtgcatgtag
     2581 tttacttttg acatatcttt ctatgcagaa tcgaggtctt attattgatg accttactgg
     2641 tgtactcaat agacaatctt ttattcaaga gctagatttg aatatcaata ctgaacaatc
     2701 aggatttatt attacaattg cgcttgatga ttttaagttt atgaatgaaa cttttggaac
     2761 aaaaaatgga gatattgcac taaaagaagt tggaaaatat ttgattcaga tacatgatgt
     2821 aaaccatgta tatagaatag ggggagatat attttctatt gttgtcaata gtcaattagg
     2881 tatagaacca gatgatatta tcagaaagat agaagaaaga tttaaaaagc cttggctagt
     2941 agaagagata agttttaatt tagcaactag tattgctgtt gtttattacc cagaaaatgc
     3001 agaaacgaca gaagatgtaa ttgttgctat agatttctct atacatgaag caaaacgctc
     3061 tgagtcaagg cgagttgttt atgcagatgc gtctataagt gaaaagataa agaagaaaca
     3121 tgttattaaa aattgtttaa aagaagcatt aaaaaatgat gggtttgaag tgtactacca
     3181 acctatattt tctaatagca ggggcaggtt tacttcagca gaggcattac ttaggctaga
     3241 acacaaagag ttaggtttta tacctcctga tgaatttatt ccaattgcag aacaaacagg
     3301 attgattaac tcaattggat tagtagtctt tgagaaggta tgtagattta tagctagtga
     3361 tgagtttgaa gatttaaaat tggataatat agctgtgaat ctttctgtag tacaatgtat
     3421 gcaaaaaaac ttagctgatg atttacttta tcttatgaat aaatataatg tatcaccaac
     3481 taaatttaaa cttgaaatta ctgagactgt ggctatgggc tcttttaata taattaaaga
     3541 aacaatggaa agattaattg atttaggtgt taagtttgca cttgatgatt ttggaattgc
     3601 ttattctgga gttactaata tgctttcttt accattttca gtaattaagt tagataagag
     3661 tttgatttgg tctatgaatg aagatagtag gcataaactt acagttgaga cgattatagc
     3721 acttataaat aaattgaata tgaaggctgt agcagaaggt gttgaaacca ttaagtgtgc
     3781 tgaagattta atagctatgg agtgtgaata tttgcaaggg tattattttt ctaaaccagt
     3841 tccagaagat gtttttaaaa atttgttgaa agaaaatact tttacaagtt aacacccttt
     3901 atttttttaa ttacaacttt gttcattgac actgccagcg atatattata aaattaacac
     3961 aataataaat attaaattta ttatacatat tattaatatt aattaatgga gtaaagattt
     4021 attatatatt ttatttaatt atcctaaagt gagaaaggaa gatgtgacat gaataaaaag
     4081 aaaatattag tgccaattga tgggacagaa agaagtatgc attctttgga atttataaaa
     4141 ggaatattta aaaaagatga agttgaagta gaaataatga atgtaaaaga actagtattt
     4201 attgatggca tatccctggc tgaagagata aaaaattctg aaaatttagg aagaagaatc
     4261 ttggataaag cagcagagat aatgggagag tatgatgtta aagtacattt tacttttgga
     4321 tatccaggag atgagataat tagaaaagca aaagaagata atgtagattt tatagttatg
     4381 acaaaatcta ctaaaaaagg tcttacaaga atgataggtt cagtaacagc aagtgtagtt
     4441 aaacaagcaa aatgtatagt gatgattgta ccggagtaga attagaaagg tatttgaaat
     4501 atgaataaaa agatagtagc agtttgtgaa agtgaaagaa gtttaaattc tcttaagaca
     4561 gctgcaaatg atttaggatt tgatttagtt tatgaaattc aagaaaatga taatataaaa
     4621 aatgaacttt ctatttatga tatagaagat gcatccatag tgttatttgt tacaagtgat
     4681 tctatagaaa gcatagagaa gattgataga tttatagata gagaatttta tgaagttgat
     4741 ccaaagtata taatagagga tgcaaaatct atagtaagtg aaattttaat agacttaaat
     4801 taatcgtggg tgtcgcacta agaatagagc gacgcctttt tatatatttt acacaagaaa
     4861 taaagaggtt gtatcaaaat atagataaga gtctttttca ttgttgaaat gatgaaaata
     4921 tatttgattt catacaaaaa agagtacctc ataactaaaa agttcacttt gagacactcc
     4981 atttttatat gttagttgca tttaaggtaa aatagaagtt taatcatgtt gaaataactg
     5041 aattacatct tctaaatgat tgtacattga tgtataacct aagtaagagt ctttactatc
     5101 aaatgcaatc attatcttgt tatggtggct aatcatatcc ttgagagaat ttctatattt
     5161 aaaaccaaag tattggttag aagaaactgt tttaggtatt aactcataat attttataaa
     5221 aattggatta tgagaagcta aaattatctc tttgtgaaat tgcaagtcag catcataaca
     5281 ttcttctatc aaaccatgag aaaccttatc caattcctct attgacttta ggtaagtata
     5341 ttttattttt tgaagttctt tagatgtagc atttaaaatt gctagtttag tagcttcgct
     5401 ttcgataagt tgtcttgctc ttaaagattc tacataaaat tcttgtatgt tatgattttt
     5461 atagatttca ccatcatcaa ataggtcaac ttttttaaat ccttcaacta taggacttga
     5521 ataaggcaca gctctaagta ttccttcctt acagagtata tttatggcat ttcttacagg
     5581 tattctactt atgttaaata ggtcagcaat ctttctctca cttggtaatt tactattaat
     5641 tttaaaagtt ccatttagaa tgtttaattt aatataatca ataatttttt cagtggcatt
     5701 taagcttttc ataaaaactc ctaaacatat aattttttta tgattgtatt atatcatagt
     5761 taagagttaa atctactttt ttataatatg tataagacag gatagaggcc aaaatagcta
     5821 taatagccaa gataaagtat ccatattgta ttgaagtata ttccgatatt ttaccaacta
     5881 tcattggaaa tacagacatt cctatagcat aaattgcttg aaaaaaaccc attgcagtag
     5941 atttctgatg ttttggtaca tttttcatag actcgctagt aaggtaagaa aggagtatac
     6001 ctgtagatat acctggtagt acttgtagta caaatattat attaatattt tttattgttg
     6061 gaactaaaaa gcaataggct gaaactaaaa taaaaactaa aggaatccaa aattttggtc
     6121 catacttagt acaaaattta gtagaagcaa agccagaaga agcaacagca cacaccatgt
     6181 aaaatataga agatagacct atgagggtag aactagaacc taagttttta agaatttgag
     6241 tagtaaatga cattgttgta gacatttgaa taccctgttg aattaatgcc ataaaggaaa
     6301 aaaatacaag tcttttatcc ttgcagatag ataaatattt tttaattgat attttttcag
     6361 gtattttttt agaattatga ttttttatat ttaaggctag aatcgttcca ataactgaag
     6421 acaatacact catgatacat aaggctgtca ttcctaattt ttcataaaat aaagtactca
     6481 taacaaatcc aatcaacatt cctagattgt ttaccaatat tatcgtactt gtagcttttt
     6541 gttgatgttc tgtagaaaaa aagttggtat agtgaaccat gtaagaaatc caagttgatg
     6601 atgatattcc agataaaatg ttagctataa gaaaaccatt gcctccagga gaaaagactc
     6661 ttataagaga tgctaaacca gcaaagaaaa ttccagacag tataaatatt ttatgtttat
     6721 ttatggaatc agcaaatact ccaacaggaa gccttaataa acattgtgat attccatatg
     6781 cacctattat tataccaatc atatttgtag atacattaca tgcacttaaa tatggatttt
     6841 ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt aacaatagaa agtaaaagta
     6901 atttttgatt agatattttt ttcatattat cctccatgtg gtatttttat aatgcaaata
     6961 atataccatt tagatttttt aatcaagtac tttctctatt aaaaaaaatt taaatatata
     7021 attattattt acaaaaaaat tatactttat agttgaatta agaaaacgct tatgatacta
     7081 taaagttaac aaatttgaat atataacagt agacgtgtta ctggtaatgc aggcaagatc
     7141 gaatcaattt ttatagatta tctatagagt actatgggta taatctattt atttggttcg
     7201 atttttttgt ttaattggcc ataaaacaaa aagcctatag ttgaaaatgg aggatttttt
     7261 atggattata aaaatatagc tcaagaaatt ctactaaatg taggaggaaa agagaatgta
     7321 aatgaagtga ctcattgtat gacaagactt agatttaaag tcaaaagcgc ttctaaagtg
     7381 aataaggata aattatctaa aactgaagga gtaataactg ttgttgagag tatgggtcaa
     7441 atacaagttg taattggaaa taaagtaaaa aaagtatatg atgaggtcat aaaaattgta
     7501 cctcaatcaa ataacatagg taaaaagaat gaaagtcaag aaaagcaagg tataattaat
     7561 tctatattat cagcagtagc tggtatattt acacctacaa tacctgctat agcaggggtt
     7621 ggtatgatta aaggtatttt atcagttctt gctatgtatt atatgaataa aaatggagta
     7681 gatataaaag aaacgcaaag ctatattatc ttaaatgcta tggctgattc tatattctat
     7741 tttatgccta ttatactagg atatactgcg gctaaagtat ttaatgcaaa taaaataata
     7801 tctatggtat taggagctac actttgttat ccaactttta ctgctttgat ggcaggtgaa
     7861 gaatctgtta gatttttagg attggcagta acaaaagcta cgtatacatc atctgttatt
     7921 ccaataatta ttgctatatg ggctctttca tacgttgaaa aagtattaga aaagtatata
     7981 cctgaggtaa ttaaaataat aatggttcca actttatcat tggtagtaat gttacctgca
     8041 acattatttt tatttggacc aatcggaatt tatataggaa atgtcataaa cttttcttat
     8101 aagtatattt atgaattaag cccagcattg tgtggagcat ttgtaggtgg gttatggtgt
     8161 gtgttagtta tatttggtgc tcatagagca ttattaccaa taggtataag tgatgttgct
     8221 caaacaggaa gacaaaattt attggcgttt gcaggagctg caaacttttc acaagcaggg
     8281 gctgctctag gagtattctt taaaactaaa aatcaaggac taaaaacaat ttctatgtct
     8341 gcaactataa cagctttatt tggtataact gaacctgcaa tatacggtgc caatcttaga
     8401 ttaaaaaaac ctatggtatg tgccgtaata tgtggtgcat ttggaggtgc gataatggga
     8461 atgggtggag cttatggtaa tgcatttgca aaccaaggtg ttttaactat acctgtatat
     8521 gctgaagcag gtgcattagg tttcttaagt tatctaggtg gttgtgctat agcatttttt
     8581 ggttcagcta tttcaactta tctagttgga tttgaagatt tggaagagtc ttctaataag
     8641 gaaaattcat ctataaaagt agaaacgaag gatggtgtta ttgatataac ttcacctgta
     8701 gagggcgaat gtatagaatt gtcagaagta aaggatgatg tatttgcttc aaaagctatg
     8761 ggagaaggta tagctgtgtt acctacaaaa ggagtaataa cagcccctac tgattgtgaa
     8821 gtagcttctt tatttccaac attacatgca ataggattaa aattagataa tggagcagaa
     8881 atgttaatac atgttggaat caatacagta gagttaaatg gtaaatactt tacaaaacat
     8941 gttaatcaag gagatttagt gaaaaaaggt gataaactta tatcgtttga tatagataaa
     9001 ataaaaaagg ctggttatga tgtaactacg ccagttatag taaacaatac atttgatttt
     9061 gggcaagtag tttcttgtaa atctagttat gtttcaacca atgataatat aatttcatta
     9121 gtattagctg gtaattaagt taatttgact agagataagt aaattgattt aaaaggaaat
     9181 aagtaaatca acaattaaat acatctaaaa gtaagtttat tgcaatgaat tgttaaagaa
     9241 aataattaat atggagtaaa tctattgcaa tgaagtaaca aagaaaataa ttaatataga
     9301 gtaagtttat tgtaatgaat tgttaaagaa aagagttaat atagagtaag tttattgtaa
     9361 tgaattgtta aagaaaataa ttaatatagg gtaaatctat ctcaatgaaa taacaaagaa
     9421 aagagttaat atagagtgaa cttattgcaa tgaatcaaca aagaaaataa tcaatataaa
     9481 aaggagaaat atttatgaat acaggatttc caaaagactt tttatgggga gcatcatctt
     9541 ctgcatttca agtggaagga gcttgggata aagacaataa aggaaaaacc gtagccgact
     9601 ataattcttt taaaaagtct catttacagg cagatactaa agtcgcatca gacttttacc
     9661 ataattatga agaagatatt gaattaatga aagaattagg gatgaaaaca tatcgttttt
     9721 caatttcatg ggcaagaata ataccagatg gtgaaggaga gataaaccag aaaggtttag
     9781 aattttataa taagataata gacaaactga tagagtgtga tattgagcca ttcgtaactt
     9841 tataccattt tgaccttcca tttaaattag tagagaaata taatggttgg gaaagtagag
     9901 aaactgtcta tgcttttgaa agatttgcta aaatatgctt taagcatttt ggagataggg
     9961 taaagtattg gcaacctcat aatgaacaga atttaattgt tagagtggag gagagaatta
    10021 atatatatga tgaaacagat tcatggaaga tagataaaat gcgtgctcag atggattata
    10081 atctttgctt agcacatgca ttagcagtca atgcatgtca tgagatgata aaagaaagta
    10141 aaattggagc agcagtatca tcatctgtaa catatcctct tacttctaag ccagaagatg
    10201 tatatgcagc tagaatgaat gataatttta aagtgtacta tatgcttgat atgcatcatt
    10261 atggagaata tccagggtat tatatgaagt atcttgaaaa aagaaatata gttcctcaca
    10321 tggaagatgg ggataaagag atacttaaaa aagctaaaat ggattttata gctgtaaatt
    10381 attataggac aaattgtgca gaagctttac ctgaagattc acaacatcca tttggattaa
    10441 gagaaggaac tgttgatttt agtatgtatg gactatttaa aatgtcaatg aatcctaact
    10501 tagaggcttc tgagtatgga gcagcaatag acccatcagg tcttagagtt gccttaaatg
    10561 aatattggca aagatatcac ttacctgtta taataacaga aaatggtcta ggagctaaag
    10621 atatattaga agatggaaaa attcatgatg actatcgtat agactattta agaagtcata
    10681 taaatgcatg taaactagct attgaagatg gtgtagagat gattggatat tgtccatggt
    10741 catttacaga ccttttaagt tcttctcaag gttttaataa gcgttatgga cttgtttata
    10801 taaatagaac tgaccatgaa gttttagatt taaaaagaat caagaaagat agtttttact
    10861 ggtataaaga agttatagag aacaatggaa tagtaaaata aacataaata tatattgagg
    10921 gttttagtat gtacattaca aaggtattaa ataattcgtt gctacttgca aaggatgata
    10981 atgatgaaga aataatattg atggggaaag gcattggcca taattataag gccgggtctg
    11041 agttaaataa aaaagatata gaaaagattt ttgttcttca tgatgaaaat ataaaaaaga
    11101 gtattataca attggcaaca gaaatagatg cagaatattt tggtatagct caaatgataa
    11161 ttgcatatgg aatagaaaaa tataacttaa aacttatgaa ccatatatat ttggccttaa
    11221 ctgaccacat tgcctttgca gtgagacgtt ttaaagaagg tataatgatt gaaaatcact
    11281 atttgttcga aatcaaggaa tttaatccaa aagagtatga tataggaaaa tatgctatta
    11341 ctgtttttaa ggaggttctt ggtttagacc taccagaaga agagattggt aatatagcag
    11401 gccattttat aaatgcacaa caagataatc cttatagtga tagaaataag agaagtgcta
    11461 aaattgtaaa tgctattttg caaatagttc attatcactt ttctattgta tataataaag
    11521 agtcatttta ttataggaga tttgtaatgc atctaaaggc cttttcacaa agatttctgg
    11581 ctaatgaacc atcaagagaa aaaatagatt ttatatatga gcaagttcaa aaaaattgta
    11641 aggaagagta tgaatgtgtg aaaaaggtag gagcttatat gtataaggaa tactccagag
    11701 aattaccaag acaagaggag ttatatttga tgattcatat tcataagatt ttaggagaat
    11761 tatgcgaaaa tagttagaat atcttaatat gctagtaata ttataaaaaa ttaaaaatta
    11821 actgcacttt aaatatgaat aattgcatta cttaaggggg ttgtcttaaa attgattttt
    11881 ttaattaaat ttgaagctca actccttttt taaaccttta tatcattaca actttaatgt
    11941 ttatacgact gtactttaga tgtatatcta ctttttacat ttctcaacat gcaaacctac
    12001 cattttttgc agtatgttaa ttgaaataaa ccattgagtt aaccctaaat ttattctgga
    12061 cttataggaa tagttccaaa gatactttaa aatattttct ggaataattg cttatttcac
    12121 atatgtttta ttgtgatgtt ttgatttaat aaattcttca actctgtata tagtaaatgt
    12181 attatcatgt ttgttttatt caagcacact tataatacgc ttgatttttt cacaatgatg
    12241 aaatatacta atgggtgtta taggtataag aatcgcatta cacttgaaga agtaagtgat
    12301 aaatgaggta taaatcctca tatgataaac tactatttca ttgaggaaca tattctaagt
    12361 gatgtcaaga tactacaata tgactaaatt cctagtgata aaaaagtcta cgaatgagtg
    12421 tagaaagggg aactaagatg aataaagttt taattataga tgatgataaa gatctttgta
    12481 ttttgttaaa aaagagtatt tcaattgaac agattgaagc aaactattgc cattctggga
    12541 gagatggact ggttgaactt tcaaaacaag attatcagtt agtagttcta gatgtgatga
    12601 tgccaggtat ggatggtttt gaaactatgg aacaaatacg aaaatatagc agtattccca
    12661 tcttaatgct gacatcaaaa agcgataatt acagcaaagt gcatggattg cgagctggtg
    12721 cagatgacta tctaaccaaa ccatttgaaa ttacagaatt tactgctcgt gtactgtctt
    12781 taatccgacg ttacacccgt tttaatccaa aaaatgagga gccacagcaa ctttctttca
    12841 aaggtttgtt gattgacctt gaccagcata cagttactac caatggcgaa cctatgttgc
    12901 ttccacccaa ggaatttggt gtacttcttt actgtgcaag gcatcagggt aagattttga
    12961 caaaacagca gatttatgaa gccgtgtggg gtgagccata tgtatatgat gacagcaata
    13021 tcatggctat tatcagtcgc ctaagaaaaa aaattgaact agacccatca tctcccatct
    13081 atttgcaaac aataaaagga attgggtatc aattcaacac ggaggtatga aaatggatga
    13141 acaaacgata attcttttct tactcttgct gtcgattggt ctactttttc tttccatatt
    13201 tgctttttcc aagcttgggc atatttacaa gcgcctaaaa gatatagagg agatactggc
    13261 tgatgtacaa attggagacg aaaatcgtaa aatactaatt aagccatgtg atgttatggc
    13321 tccattggtt tatcagctta atgagattgt ctacgattat gaaaataagt tactttctct
    13381 gaaaaagtcg gataaagcta gcaaacaact tatgacaagt ttatctcatg atgttcgtac
    13441 accattaaca actctgattg gttatctgga tgctgtacac agtggtatag ttatcgaaca
    13501 agagcgggag gaataccttg aaatcgctag gcgtagagcc tacgacctca aggattacat
    13561 tgatgttctt tttgactggt ttcggttgaa ctctgatgaa tttacgctgt caatagaatc
    13621 tgtggaaatt gcagagttgt cacgcaatat tttgaaagac tggattccta tattccatga
    13681 aaagaaactg aattttgaaa ttgatattcc agaaaatcga ctaatggtaa acctcgaccc
    13741 agatggatat tcccgtgtgg tcaataatct ggttcaaaat gttcttgctc acagtaaggc
    13801 tcggcaaatc aagattacca tgagtgagga ttccagaatg gtattactcc gtgttgagga
    13861 tgatggtgtt gggattgcca gagaaaattt accacatatt tttgagcgac tgtataagtg
    13921 tgataaagga cgttcagaaa aaggaagtgg tcttggtcta tctattgtca gtcaaatggt
    13981 ggagcgaatg ggtggtcagg tttccgtaga aagtgagatt ggaaagtata ccgtatttac
    14041 agtgagctta ccattaatat aaaattgttt ttcccccatg tgacatgtat acacatgggg
    14101 attttttgta agttataaag ttgcaaggtt aatacaaggt tgttgcaagg ttaatgtaag
    14161 attgaggtgg tatgataact ttaataaaca aaggaggctt ttattattta tgaataattt
    14221 aattattgaa acaaaaaatc tcactaagca atatggtgct caaaagagcg tttctaattt
    14281 gaatattcac gttcagaaag gtcgcattta cggattgcta ggaagaaacg gtgcgggtaa
    14341 gacgacaact atgaaaatgt tattgggtct aacaaaacca actactggag aaattaaaat
    14401 atttggtcag gacattcaca ataacgagaa gaagcttctt cctcgaattg gatgtttgat
    14461 tgaatcacca gggttttatc ctaatctgac aggaacagaa aatctcaaaa tctttgcaag
    14521 gcttagagga ataccaaaaa aaaatgcagt agaatcagca ctggatgtag tcggtttgcc
    14581 aaacaatgac aaaaagttat tttctcaata ctctcttggt atgaaacaga ggttggcgat
    14641 tgctcttgcc attatgcatg acccacaact gttgattttg gacgagccta tcaatggact
    14701 tgatcctatt ggtattgcag aagtaagaac gtttattcgt gatctctgca cagaacgagg
    14761 taaaactatt ttgatttcca gtcacattct ttctgaaatt gccttgttgg cagatgacat
    14821 tggcattatt gaccatggta ttttgttgga ggaagaaagt cttgcccaat tagaagaaaa
    14881 aaacagtaaa tacatacatt ttgctgtatc tgatgcagca caggcagcaa ggcttctgga
    14941 aacccaactt aacctgaaaa atttcaaagt agcagataat tacaatatta tgctatatga
    15001 tacagatgtt tctgttgcag aaataaatcg tgctttcatt ctaaacaaca ttgaggtatc
    15061 agaatctcat ttatgcgaag atactcttga agattacttc aagaaagtaa cagggggtga
    15121 aggaattgct tgatataatt tcatgtgaat tttcaaaact caagcggtta aaatttatac
    15181 tgatttctat tttaggtgct tgcttatttc caattcccgc aacaattttg attgcaaagg
    15241 acaacttgcc ctttgagcaa ttgttcaaat tagttgtcaa ttttggatat tttttgctat
    15301 taccaattgt attaagtatt gttgctagcc aactattttt tatagaaaga gataatgatg
    15361 tattaaaaaa tcttgcaaca gtgccagtgc aaaaaggaaa actggctctg gcaaaattgg
    15421 ctgtgttgct gtttatatca ttgttctact ctgttgcagg gttaggagct actatcattg
    15481 gaggtttcat tgttggaata gtagaggggg ttgctgtaaa attaggaatg agcattgctt
    15541 ttggaattat gttttttgtt actgtacttc ctgttgtggt actcattgta tatttcaatc
    15601 gcagctatat tttttctatt atactttctt ttgtatatgc tattttcaat tttagtatat
    15661 ccttaaatat tatcaatttt gaaccaaata acccaatatt aagtgtatta cctgctcctg
    15721 ttataatgcg ttggtggatg gcattttggg gagatcccac tggcgaatac acggcactta
    15781 gacagccata tttgctctca actcctgctt gtgtaggtat tttatgtttg ataaccatta
    15841 tagcagtcct gctgatttgt atgatataca aaaagcagga gaattaggag gtatctatgc
    15901 ttgatataat tactacagaa tttcaaaaaa taaaaagata taatattcta tggattggtg
    15961 ttgttgctgt tctgttttca gcactattgg cagtttttca ggttgtaagt tcacacggca
    16021 gtgaccctct aacgtatgag agctttgcaa acggagttat ctggaataat ttcagtctta
    16081 gttttccatt tgggattaca cttattggag gatatcttat taaccgtgaa tatacagacc
    16141 aaaccttaaa aaacatccta actgttccta tttcattacg caaattgcta gtgggaaaat
    16201 taattgcggt tggaggaatt actgtattgt taagtctttt cagcttctta tgcacattta
    16261 ttttggggtt gttgttttgc aatatcaata tatctgttac actcgttgta aaatccttag
    16321 ttcagatttt gtctgttaat ctgtattgtt atgttgctgt tttgccgatt attgcttatt
    16381 ttggtcgtaa acaaaatgca tttctgacgg gtgtagggat tgcatttgta tacggttttt
    16441 gtggtgtgtt tgttgctgga agaaatctga cagactttta tccaatcaca gcaggtcttg
    16501 gtctagttgg atacaacaat ggtgctggta ctgtttatca gccacttatc ggtgctatga
    16561 ctttgattgt aattctaatc ttgacaacta ttctacttgt atttacacct aattatgata
    16621 aagttatgac catatctaag aaaaaaggga taaagcataa aaagtattct cataaataac
    16681 ataaatattg tttctattat ttaagttgct agacagtgaa aagaaaaaat ctgtcgtaca
    16741 gtgtctatat gctcatgtat tcaagaagct acagttttga tttttcataa ctgtagcttc
    16801 tttttgtatt tattaaatca cgactatcat aatacttgca ataggtatag cgacaagaga
    16861 gagtactgtc atatgtgttt tatatattac attaagtata ttctaaaata tatgttttaa
    16921 ataatatatt tgaaaatagg aatatttaaa taaaaaaaat aatattatat atagttgaca
    16981 aaaattggca aatgagataa tatataaata tagaaatagt aatcattact attattaaaa
    17041 agggaggaat taattatgaa cttaaaagga actaaaacag aaaagaattt aatggcagga
    17101 tttgcaggag agtcagaagc aagaaataaa tatacatact acgcttcaaa agcaagaaaa
    17161 gaaggttata atcaaatagc tgctatattt gaagaaactg ctaacaatga aaaggaacat
    17221 gctaaattat ggtttaaact tttacatgat ggtatgcctt caacagagga aaatttaaag
    17281 gatgcagcag caggtgaaaa ctatgaatgg actgatatgt atgcaaaatt tgctaaagaa
    17341 gctagagaag aaggatttga caagatagct tatttatttg aagctgttgg aaaaatagag
    17401 aaagagcatg aagaaagata cttaaagctt ttagaaaact taaatgaagg aaaaatattt
    17461 aaaagagatg aagaagttgt ttggcaatgt caaaactgtg gtcatgtgta tgtaggtact
    17521 gaagcaccag aaaaatgccc agtatgtgac catccaaaag cgtatttcaa tataaaagct
    17581 gaaaattatt aaaaattatc attattaaaa aggaatgtta actatgaaat tttctaaaca
    17641 acgagaactg attttaaatg aaatattaaa taatccggtt catcctactg cggattacct
    17701 atatgaaaac ttaaaaaaag ataatccaaa tttaagctta ggaactgtgt atagaaattt
    17761 agctcagcta acggaacatg gctttataag aaaggttagt attccagggt atccagatag
    17821 atttgatggt cgaatagata atcactatca tatcatatgt gaagtatgtg gagaggtata
    17881 cgatttagaa tccgaagttc ttaataattt acaagaatta atatctgagg agacagacat
    17941 aaaaataaca tcttataaca taagttttaa agggatttgt aataattgta aaaggtgtag
    18001 ccaagtaggt tagttttcaa attctaaaaa taattaatga ggtgagttta tgtgtagtga
    18061 acaaaaattt tttatatgtg agacttgtgg aaaccttgta ggaatgatac aaagtggtgg
    18121 tgtacctatt ttctgttgtg gaaaacctat gaaggaactt gttcctaata caacagatgc
    18181 agcagttgaa aaacatgtac ctgtaattga agtggatgga aataatgtta ctgttaaagt
    18241 aagtagcaca actcatccaa tgactaaaga acatcatata gcatgggtgt atcttatgac
    18301 tgaacaaggt ggtcaacgca aatgtctagc agtagatgga gaacctgtag ttaaatttgc
    18361 tttgaatgat gatgataaag tgatttctgc ctatgcttac tgtaatttac atggattatg
    18421 gaaggctgaa ctgtaatatt taaataaatt gagtcaaaaa ggaggctata aatgtatttt
    18481 catacaaata gcctcctata aatttatata aattcaagtt ttaattttgt ttaatgtcaa
    18541 tataatataa ggagaaaata atatgtcaat ttataaatgt agtgtttgtg gatatattta
    18601 tgatgaatct aagaatgata aaacatggga tgaattaagt gaagattggg aatgccctgt
    18661 atgtacaaaa ggtcgtagtt attttggaaa gataagtact gtttactatg aagaagatga
    18721 aaagatagca gaagacatag ttgaagatga atctaaatta aatactgaaa aagagggaga
    18781 tttaaattac ttaagtacat atttaagaag agatgatgaa gttgagaaac atatggatat
    18841 aattcacgaa atggctgtta caggaaaatc tataatagaa cctatgagaa ctaaactacc
    18901 agtaatttct tgggatgata ttttaattat gggtgctcaa ttaaatccat tacctttaaa
    18961 tgagcatgat gaagtaaata caacaacaat aataggaaaa aaagcaaaga aacctatgat
    19021 tatagaaaat ccagtttata tttcccacat gtcatttgga gctttgtcta aagaactaaa
    19081 aatagcttta gcaaagggtg cagcccaaaa taagacagct atgtgtagtg gtgaaggagg
    19141 tatattacca gaagagaagg aagcttcata caagtatatt tttgagtatg taccaaataa
    19201 atatagtgta actgaagaaa atcttaaaaa ttctgatgct atagagataa aaattggtca
    19261 aggaacaaaa cctggtatgg gaggtcatct tcctggcgaa aaagtaactg aagaaatagc
    19321 aaaagtaaga aataagcctg taggtcaaga tgtaataagt ccatcttgtt ttgaggaaat
    19381 acaatccaaa gaagatttaa agaaacttgt agatgaactt agagaagttt cagaagggcg
    19441 tccaataggt gttaaaattt cagcaggtca tattgaaaaa gatatggaat ttatagctta
    19501 tgctaaacct gattttgtaa ctattgatgg tcgtggtgga gctactggag caagtccaaa
    19561 gttgcttaaa gatgcaactt caattccaac aatatttgct ctttatcgtg ctagaaaata
    19621 catagataca catggattag atatagattt ggttataaca ggtgggcttc gtatatctac
    19681 tgattttgct aaggcaattg ctatgggtgc tgatgcagtt gctattgcaa gttctgctct
    19741 tatggcagcg gcgtgtcaac aatatcgtat atgtggttct ggtaagtgtc ctgtaggagt
    19801 agctactcag gatgaggaat tgagaaaaag attacatata gaaaacagtg ctaatagagt
    19861 ggcaaacttt ttaaatgtaa gtttagaaga acttaagact tttgctagaa tttctggtca
    19921 taaagatata catgatttaa gtgttgatga tttatatact gttaactctg aaatatcaaa
    19981 ttatacaaac atacagcatg tatagattga agtttgaaac tattagattt tagatagcta
    20041 taaaagtaaa tgataaatat attttataaa agagagtgtc ttaaaatgaa ctttttagtt
    20101 cataagtcac tctttttttt atgaagccaa atatatttat cattttagca atgaaaaaga
    20161 atcttatctc tttattttga gatagactcc ttctatttat gaaaattaaa gatattaaat
    20221 tgtaaaatat tctgtgatgt cgtactatta aactaaagat tctgtacttt aaagtaaata
    20281 aaaatttaag gagtgattat tatggaaaga ttgaagatac aagttttagt tgacaataat
    20341 acttatattg atagatactt tgttggagaa ccagcagtaa gctattatat tgagattgat
    20401 ggaaatagga ttttgtttga ctcagggtat tcagatgtat tcatatcaaa cgctgaaaaa
    20461 ttaaatatag atttaggaaa tttaactcat gttgtttttt ctcatgggca taatgaccat
    20521 acaagaggta tccagtttct agaaaatagg tatgatttat ctacagttga acttatttca
    20581 catcctaact gttttatacc tagaaaacat ggagagaaaa gcataggagc tccattttct
    20641 gctgaggaaa ttaaaaatat ttttatgtat aatcctaagg ataagccatt taatctaagt
    20701 aagaattgtg tgtttttagg tgaaatacct tctataaatg attttgaaaa aagagctaaa
    20761 attggtaaat gtaaaatagg agatttgtgg gaagatgatt atgttttaga tgattctgct
    20821 atagtttgta aaactgataa aggtattttt attgttacag gatgttctca tagtggaatt
    20881 tgtaatatag tagagtatgc taaaaaggta tgtggagatg atagagttat tggtatacta
    20941 ggtggttttc atctttttga attgaataat aggttagata gtacaataca atatttggat
    21001 aaagaagaaa tgagaatgtt gtacccttgt cactgtgttt cttttaaggt aaaagcgaag
    21061 atgaatgaaa tattgaatat aaatgaagtt ggagtagggc tgaccataag tatatagtat
    21121 ttgaaagtat ataagattta agttgaaggt taaaaagata ttttctatag aaaatatctt
    21181 ttt
//
LOCUS       DAYASV010000063        20986 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYASV010000063 DAYASV010000000
VERSION     DAYASV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.063"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(441..2828)
                     /locus_tag="ACGI68_002205"
     CDS             complement(441..2828)
                     /locus_tag="ACGI68_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0138640.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTEPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKAYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="ACGI68_002206"
     CDS             complement(2851..3216)
                     /locus_tag="ACGI68_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0138641.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="ACGI68_002207"
     CDS             3415..4263
                     /locus_tag="ACGI68_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138642.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGIPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSIQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="ACGI68_002208"
     CDS             complement(4700..5788)
                     /locus_tag="ACGI68_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138643.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="ACGI68_002209"
     CDS             5913..6641
                     /locus_tag="ACGI68_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0138644.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="ACGI68_002210"
     CDS             complement(6843..7334)
                     /locus_tag="ACGI68_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138645.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="ACGI68_002211"
     CDS             complement(7345..7788)
                     /locus_tag="ACGI68_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138646.1"
                     /translation="MYSNTYNNENFMRARAFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="ACGI68_002212"
     CDS             complement(7778..8617)
                     /locus_tag="ACGI68_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HFH0138647.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="ACGI68_002213"
     CDS             complement(8836..10221)
                     /locus_tag="ACGI68_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138648.1"
                     /translation="MENLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKYGFSIDELEGMDTAFAASYGEGRPVIAVLGEYDALDSLSQKVSTIKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="ACGI68_002214"
     CDS             complement(10394..11137)
                     /locus_tag="ACGI68_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFH0138649.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSKHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="ACGI68_002215"
     CDS             complement(11157..12419)
                     /locus_tag="ACGI68_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138650.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="ACGI68_002216"
     CDS             complement(12603..13601)
                     /locus_tag="ACGI68_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138651.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="ACGI68_002217"
     CDS             complement(13585..15096)
                     /locus_tag="ACGI68_002217"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0138652.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="ACGI68_002218"
     CDS             complement(15150..16112)
                     /locus_tag="ACGI68_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0138653.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI68_002219"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="ACGI68_002219"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFH0138654.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18114)
                     /locus_tag="ACGI68_002220"
     CDS             complement(17092..18114)
                     /locus_tag="ACGI68_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0138655.1"
                     /translation="MNFLSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKI
                     MKEKNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNT
                     DEDAKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETL
                     KGIVKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYN
                     PAYVFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVV
                     GFDDIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRD
                     STKESE"
     gene            complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI68_002221"
     CDS             complement(18334..19311)
                     /gene="bioB"
                     /locus_tag="ACGI68_002221"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFH0138656.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19517..19780)
                     /locus_tag="ACGI68_002222"
     CDS             complement(19517..19780)
                     /locus_tag="ACGI68_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138657.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19850..20923)
                     /locus_tag="ACGI68_002223"
     CDS             complement(19850..20923)
                     /locus_tag="ACGI68_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0138658.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
BASE COUNT         6727 a         3857 c         2130 g         8272 t
ORIGIN      
        1 cttaaaaata attcgtcaat acttttttaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaatgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatgcttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaagaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctcgaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgctaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggctctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ctcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcattcc taacaatgtt cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggta tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctatac aaggtatacc acaggctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtattgg tgggtcagaa
     4501 tagtatgata ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gccaaaaaat
     6301 ataatgtaac cgatttttta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatatttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagcttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaagccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgctcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaac cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcataattca aagaaaacct agagatttct ccatgatttc
     8521 tttttaaata tttacatagt ccacaatagt aagctttgta ataatcgtat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttgtgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttattta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcttt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg taactaacat ctccaatatc caatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg tacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg ggattcttat aggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcctct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta cccagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttaata gtggatactt tttgagacaa
     9961 actatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccatagct
    10021 agccgcaaat gctgtatcca taccttctaa ttcatctata ctaaaaccat atttttttaa
    10081 gtattctttt tgtaaatttg aagcataata ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattctcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctatc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgtttag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccgaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaatcc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattaa tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 tttttcccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gtccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacctgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatctacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattcc cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaaaat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcttcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcccc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattctgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatactt ttcaacatct tctttatagg
    16201 gcataccaga ttgtgcacct agtttagtta cagaaagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tatttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattacact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg taaccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgca aaaaaaaatt aatatacttt tataattatc
    18301 taaaagtata ttaattaatt taagcagtat ttattaaagt tcaaatcctt gctctataat
    18361 cattttttta tcattttcaa tcccacttcc aacagttgtc aaataatcac caacaattgc
    18421 agaatttact cccccattaa atccaacttt gtcatatccc tttaatgata gccttccacc
    18481 agcatacctt atgtacacat taggaattat aaacctaaat actgctgttg tttttagtat
    18541 ttccattggt tctaatacat cataattttc cattggtgta ttcttaatag gatttaaaat
    18601 gtttattgga aatgatttta catttaattc tcgtatttca aaagccattt ttagtctctg
    18661 ttcttcactc tcattcattc caattatacc acctacacat acatctagcc cagctttttt
    18721 agcatctttt atagttttta tcctatcttt atatgtgtgt gttgtacata tattatgaaa
    18781 attatctcta cacgtctcaa cattgtggtg atatgtagtc acacctgctg acttcaacat
    18841 tttagcttgc tcataatcta taatacctaa agatgcacat aattttaaat tagtattttc
    18901 cctcaatcct tcatagatat ttaaaatatt attaaactcc tttcctgaca tacctttacc
    18961 acttgtaacc aatgagaatc tatgcactcc tttactctcc atctctttag ctctatttaa
    19021 gatttcattg taatctaata atgaatattc ttctacacca gttttataat gagatgattg
    19081 agcacaaaat ttacagtctt cactacattt gccagattta gcattcatta tcgtacataa
    19141 atctgctttt cttcccataa aatattctct tatttcattt gcagatttta atagaatatc
    19201 aaaatcgttt ttattatttg tatcaaggct tattaaatta agagcttctt cataacttat
    19261 ttctttttct ctcaatactt tattttttaa ttttattatg tatttttcca tatatcctcc
    19321 tatttgtcaa cttaatattt ttatgtagtt tacttattat atatagtatt gcatatataa
    19381 catttattgg tcaactttat tttagacaca agtttactat acacaaaaaa atactgcttc
    19441 aaaataatat tagtaaatgg attatacata ttattattaa aacagtattt tttattagaa
    19501 tataattctt actggtttat aaaactatcc ttgtcttaac tccatccaag ctttccattg
    19561 taataacctc taagccttca tattctctaa atagccattt taaatccttc atggcttgat
    19621 taacctgact ttttgttaga ttatttataa aatattcttt tctagtttcc tcatactctt
    19681 tgctagtcaa aaagtttctt agaaaattaa acgcaaatac acttggaata ttgtttacat
    19741 tagccttctt gctgttttcc ttctcatagt aaattttcat aaatgcatac tcctttgata
    19801 cttttagatt tctcttacaa taattatatg catttaagtg ctactattct tataatttta
    19861 ttaattcata ttctttactt cccataccca tttcacatgc atagtctatt attctagttc
    19921 catttacatg aggatgttca tgtttgaaaa catcatgacc catttcctta caaatcaaat
    19981 catagcatgc ttgttcaata gctacagggt ctgttgatgc taatattcct atatcatggg
    20041 caattggtct acctgaccaa ggtacacaat cacaaagtgg agttacattc ataacaaaag
    20101 ttatataccc taccttgtct tttttatttg atactgctcc ataagcgtat tctgccatct
    20161 tttcgacaaa cacatctgag tcactttccc attgtatagt tactgctcta gttggacaga
    20221 cagttggaca ttctccacat ccataacata catcagaatc tataactgct tttttatcca
    20281 ctatacttat agcttttgta ggacaagaat taacacactt tccacaacct acacacttct
    20341 tttcctttac tactggagta acatcagaat gttgcatttg ttttcctgct gcacttgcac
    20401 atcccatagc taagtttttt aatgctcctc caaaacctgc tgcttcatga cctttaaaat
    20461 gactcattac tatcattgca gaagaattat aaatttctcc acctattttt acactttcaa
    20521 aatgtttttt gtctattttt acattctcat aatttctact atataatcca tctgcaatta
    20581 ttactggtgc atttacaact gcataagcaa atccattttc tatagcagtt gtaagatggt
    20641 ctacagaatt tgttctactc cctgtataca gtgtatttgt atctgttaaa aatggctttc
    20701 cttcgcagtc tttaacctta tcaaccactt gtctaactgc cacaggactc atatatgtag
    20761 tatttccctt ttctccaaag tgaagtttaa ttgcaacttg gtcattcttt tctattaaat
    20821 ctttgaatcc tgcttcatca aataatcttc ttacattgtt tggtacgttt ttattttgcg
    20881 aatttgagta taaatcacaa aaatatactt ttgacttttc catatctaca cccctttaaa
    20941 atatttactt atactaattt tatcactttt aaagtaattt ttagta
//
LOCUS       DAYASV010000064        20806 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYASV010000064 DAYASV010000000
VERSION     DAYASV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.064"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(113..649)
                     /locus_tag="ACGI68_002224"
     CDS             complement(113..649)
                     /locus_tag="ACGI68_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFH0138659.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(667..1980)
                     /locus_tag="ACGI68_002225"
     CDS             complement(667..1980)
                     /locus_tag="ACGI68_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFH0138660.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2335..2775
                     /locus_tag="ACGI68_002226"
     CDS             2335..2775
                     /locus_tag="ACGI68_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFH0138661.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2959..3147)
                     /locus_tag="ACGI68_002227"
     CDS             complement(2959..3147)
                     /locus_tag="ACGI68_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0138662.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTIIKNSNKKE"
     gene            4169..4564
                     /locus_tag="ACGI68_002228"
     CDS             4169..4564
                     /locus_tag="ACGI68_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HFH0138663.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            4607..6706
                     /locus_tag="ACGI68_002229"
     CDS             4607..6706
                     /locus_tag="ACGI68_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0138664.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYTGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGETMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNTSTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(6931..12141)
                     /locus_tag="ACGI68_002230"
     CDS             complement(6931..12141)
                     /locus_tag="ACGI68_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFH0138665.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKELM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNTLYEEHKDWTISKREDSGSA
                     GAEGKAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILEFSHIYEIFYAVEKIEMQLNREILQKYYSFEKKNKAVLSMDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDIKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILRSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(12156..14651)
                     /locus_tag="ACGI68_002231"
     CDS             complement(12156..14651)
                     /locus_tag="ACGI68_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0138666.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIES
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(15312..15599)
                     /locus_tag="ACGI68_002232"
     CDS             complement(15312..15599)
                     /locus_tag="ACGI68_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138667.1"
                     /translation="MRLNITKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNIFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(15619..15894)
                     /locus_tag="ACGI68_002233"
     CDS             complement(15619..15894)
                     /locus_tag="ACGI68_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138668.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(16549..16728)
                     /locus_tag="ACGI68_002234"
     CDS             complement(16549..16728)
                     /locus_tag="ACGI68_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="HFH0138669.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGNAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(17013..18488)
                     /locus_tag="ACGI68_002235"
     CDS             complement(17013..18488)
                     /locus_tag="ACGI68_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0138670.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYVTLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRLKLGIL"
     gene            19786..20697
                     /locus_tag="ACGI68_002236"
     CDS             19786..20697
                     /locus_tag="ACGI68_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0138671.1"
                     /translation="MAVISFCILHLITVSTISLVKNTSPDIIFANMTYKLISILIASI
                     LDFILWYSMNKIKEHIYIITQNQIEFSLFLKFIWFTLGYILFDSIQITFAPPSSLTSY
                     FILGSNILLILQLIIFINHIYSIEKNIYLEKEHNRLKMIKQEQLLKVNLLKKKANIDE
                     LTSAYNRKFAFEKIQHYIDNNLLFSLIYIDLDKLKTINDTYGHLAGDNYIINFSSIVI
                     ENLGIDDIFARIGGDEFIVIMPKSNENTTKEFIEKISKIMDNTISFSYGIVEVSSENK
                     MTVEEIINIADIRMYTNKQNNHKRNNN"
BASE COUNT         7055 a         3553 c         2249 g         7949 t
ORIGIN      
        1 taattttcct actctttttt ctgtacttac atataatatt aaaactcttt attttttaat
       61 atatatttta aattttcttt tttaataaat ctttttcttt taataaatct atttaatttt
      121 cttcttctat atcagagcca aagggaatcc atgcattacc aaactcttta tctttaaata
      181 aataagctaa tccagttatt tgcttcaatc taagaatttc atcaacttcc atacccaggt
      241 gctttgatat ccattcatca gattttccaa tttcacaaag ttcagttata attttactca
      301 ttaaatccac attatgagaa cctcttgcac gattgtgtcg aatagtactt gccattcttt
      361 catcaagaga tttatcaatt actgaaacag gaagaagtcc attttctcta tcatatatat
      421 cctcattttc aagcattact ctatatctat gaaaaccatc tacaataata tacttatcct
      481 tttcctcatt ataataacag acaattggca ttgtataccc atcttctttt atacttttat
      541 acaacaactg catttctggt ggggctactg catttggatt atagtcgtta gattctatct
      601 tttcgattgg cactgcgatg attccatata caggactttt atattccata gcatcttaac
      661 tcctatctaa tgaatttttt catatttctt cttaatatat tccgttctaa ttctctgttc
      721 ttttgtagga ccaaatccca taaatctaca catatggtca ttttttaaaa tacaataaca
      781 cattcttttc caacttggta tatctttggt aaatttaata tcatccgtat catcaggtat
      841 atcatccaaa aatatgactc tactcttttt atcttttgta tgatttgaaa ctccatttaa
      901 ttctatttta taaccttttc tcaacaattc acgtataact atttctgaca aaccaccacc
      961 tgtattctgc caaaactcaa tagatgttga aaatttttta atatagctat tccttatatt
     1021 ttcaggtaag gtatcaagca aaaaatatgt gaaacttttc catgtgtgtc catatggcaa
     1081 tttaacacta cgatatccca tagcttttgt tttgccataa atagaagtaa aattaactcc
     1141 ttgtactcta gcaaccaatt tcacccatac atctgggtcc aatattctgt ataaatttaa
     1201 actctctttt gctgaatcat taaaaggtga tgccactctc atttttcttg gagctactcc
     1261 agctttatag aataagtcat aaattttatt atagtcatag tcaaatttat agtgtgctaa
     1321 ccatatatct tctatttccc aatcatatat aggagatgcc gtccagacat ttttaaattc
     1381 cttagttatc cagcactttc ctttatatcc atgctgttta ttcacaatag aacaataacg
     1441 ttgtagagat tctgctgctc gtgttcctaa taagcaaaca acttttgcac cttcatgtat
     1501 atctctaatc catctaccaa attgttttgc caaactttct tgtctcattt tatatttata
     1561 ataagtaaaa ggattatttt tttgatttat tatgtattct ttgactggca tttcccttac
     1621 ccataactct tctttctcat catcccatgg ataccaatac atttcataat ttcctacagc
     1681 agttcttgta gccattggta aacacaccca atatggttca atatcttcaa tattatcttc
     1741 aaatactttt tcaacatatt cagtagtcaa actatattga gcctcaaaat cttgatgaaa
     1801 tactcctatt ttggaactta tattattctt tctcttataa tccaaaacta aatttagcag
     1861 taaaccacta tcttttcctc cgctaaaaga tacatagata taatcaaatt cattaaaaag
     1921 ataagaaagt ctttcttgaa aagcttcaaa tacatcggat tctaaatatc tcttatccat
     1981 ttgaattatc ccctttcaat tagaagttta taaaacctta caatgcaata ttattatgct
     2041 tttttagcag taatcagcat aaattataca ttatttaaat acacaaatcc aactaactac
     2101 atataattat aatgtaatta tacttttgta ttataaacta tattttatca ttattttaca
     2161 tttttttcca atatatataa atatcatatt tttctattag ctaaaactaa ttactttata
     2221 tttctacgta ttagtataat aatacataaa aacttgaata atgcataaaa acttgacata
     2281 ctgttgtcat aattactttg ctataattaa aagtaaaaat ggaggtaaat tctaatgtat
     2341 gaacgtatgc ttgataaaag caataaaccc tgtttgtccc aaataataga atatataggt
     2401 aaagatggat atgcacgatt gtgtaagttg gaaacttact tgaaaactca atataattta
     2461 tcaagagaac ttcgatttcc ttttggaaac aattatggtt ggggttacaa atatagccat
     2521 aaatcttttc atttatttta tgcttttttt gaaaaaggtg catttactgt tctgattcag
     2581 ataggtgaca agcaagttcc agcagtagaa aaaacgttgg tagctttgac accaaaagct
     2641 aaagaactct gggtcactcg ttatccttgt ggcaataatg gaggctggat taagtatcgt
     2701 attttagaag atgcagactt acttgatatt attcaatata taagagcaaa aaaatctcca
     2761 cctaaacaaa attaactttt attttttgtg atttaaatat agtgtatata aattccattc
     2821 tttccatatt aaaaaataat ataaaaaata tctattagta taacttattt agtatgtctt
     2881 attctacttt ttacagataa ttaaaaagtt ttatcataaa taaatcataa ctagacatcc
     2941 tagtaaatca aaattaattt attctttttt attcgaattt ttgataatag tccctataca
     3001 tatgccaatt atcattccaa aaattagacc ataacacatt gtaataccta catttccttt
     3061 agaaatacct atagcacaac ctatagccat tccaagtatc atgcctataa acatacatat
     3121 aataactaaa ttgttgtttt tattcatagt ttttctccat tcaatatatt ttaaattgcc
     3181 tagctcgcct tcttagctta aaacaaagaa atataaatta actttctttc attctaatat
     3241 ctgcctgtat aaatgtcaaa caacacattt cttgtcataa cctcctcttt ttgaagtaag
     3301 tacaatctcc cagacgcaat aatatcaatt tcctaaaacc atttaagtaa cttttatatt
     3361 tacataatat ttacaatcat aaactatatt gttattcatt tatacatcat aaacactccc
     3421 tcctaatcat tattatactt aaaataatgt tcatttataa taatatatct aacgcacttt
     3481 aattgaaaaa actaaaactt tgcctttcat tcccatatat aaataagtat actttttgat
     3541 gctctattac aaatcaacta aattaattga tataaattat cattttctgt tgaaaatacc
     3601 attcgcacat gctatactta attgcataaa tggtaataaa tgtcatatga ttttacaaat
     3661 ctattaataa caattattac ttttactgta ttttaaagtt tttggactaa atatcaagaa
     3721 ctttctaaaa tacaaagtac aaaaaattta tataagttat aaatttatat gaactagata
     3781 agctcaaaca atatgttatg tataaactgt gtagtattcg gttataaatt tatataaatt
     3841 atataagcta gataaagtat tacctcaact atataagcta gatatataaa ttgcttatat
     3901 aagctagata agaattattt caactatata aactacatat ataaattaca tacataagct
     3961 agatatataa actgcatata taagctatat aagaattatt tcaaccatat aaactacaca
     4021 tgtaaaattc atataaattt actaatataa atcaaataaa attactaaac tctctaaact
     4081 aattttgtaa aacatactct atttttaaca tttgttacct tatacataag tacaaacaaa
     4141 atctaactaa atgaaaggat aatgtaaaat gaatatttta aaaatgaaaa aaacagggat
     4201 atttttagga ggagtacttt ttggtactgt aggtgttaag atactttcaa gtgattgtgt
     4261 taaaaaaact tgcactcgtg caacagcagg agcattgaaa gttaaagatt ctgttcttga
     4321 aacaactacg aaaattcaag aaaatgtaga agatatttta gctgaagcaa aagaaatcaa
     4381 cgagcaagaa gacttatcta catctaaggt aaacgttgta caagatactc ttaaatgcga
     4441 acaagaagtt cttaaaactg aaactataaa aactgtagaa gatactaatt ctaatagcat
     4501 taaatgtgaa gaaatcaaag ataacgtaat tgaagaaaaa aatagtactt gtacaacaga
     4561 ataaatttaa tgattattat gattaataat atcaggagga agtaaattga atttttatat
     4621 aaaacacgaa ataagaggtc gtatacgctt tgacttacaa cttggcaaat taaacaacaa
     4681 gcagtccgat actctcttat attatttaac ttctataaat ggtattacca aagcaaaagt
     4741 ttacgataga actggtgatg cagtcattta ttacactggt gaaagagaat acataataag
     4801 agaaatcact aaattcagtt ttaacaatag caaaatagaa aacctagttc ctgaacatac
     4861 cagcagggaa ttaaacaatt attatagaga caaattagca aataaactgc tagtcaaaag
     4921 tatctccaaa atattttttc caaagtcctt aagatttttt ttaattggac ttcattctat
     4981 aaaatatttt aaaaaaggtc tctcttccct gctaaataaa aaaatagaag tttctgtttt
     5041 agatgccact gctataggta tttctatttt tagaaaagat atcaatacgg ctggttcagt
     5101 tatgtttctt cttggtatcg gtgaactatt agaagaatgg actagaaaaa aatctataga
     5161 cgacttggct caaagtatgt cactaaacat tgaaaaagta tggttaagaa aaggtgacac
     5221 tgaaatctta attccaattt cagagataaa ggaaggagat ttagtaagtg taactatggg
     5281 aaatatgatt ccattggatg gagttatagt atcaggagaa acaatggtca atcaggcatc
     5341 acttacaggt gaatcccttg ccgttaacaa aaaagaaggc tcttacatat atgctggaac
     5401 tgtaatagag caaggcaata ttgttatgtg cgtcaaggaa aaagctggta ctactagatt
     5461 ccagaaaata gtaactatga ttgaggaatc tgaaaaacta aaatcttctg tagagagtaa
     5521 atttgaacat ctagcagata cattagttcc atacagcttt ttaggaagta tattaaccta
     5581 tgcaattact agaaacccta taaaatcact ctctatatta atggtagatt tctcttgtgc
     5641 attaaaactc tctatcccta tttcagtact atcagccatg agagaatgta acaataacaa
     5701 cattactgta aaaggtggaa aattccttga aggtgttgca tgtgcagata ctattgtatt
     5761 tgataagaca ggcacattaa caaaagctca accaactgtt tcagatataa ttacattcca
     5821 aaattataat aaggaggata tgttaagact tgcagcttgc ttagaagaac actttccaca
     5881 ctccatagca aatgcagtag tttacgaagc tgaaaaacaa gggcttagtc acaaagaaat
     5941 gcatacagag gtagaatata ttattgctca tggcatttca acaaaagttg atgataaaaa
     6001 ggttgtaata ggtagcaaac attttgtatt tgaagatgag aagtgtacta tcccagacgg
     6061 tgaggaggat aagtacaaca atctatcaga tgagtattca cacctattta tggcaatatc
     6121 aggtaaatta tcagcagtta tttgcataaa tgacccttta cgaaaagaag caaaatatgt
     6181 aatttcaaat ttaagagaat gcggaataaa aaagattgtg atgatgactg gagatagcga
     6241 aaaaacagct aaatctatag caagtaaagt tggtgttgat gaatattatt cagaagtctt
     6301 accagaagac aaagccaatt ttgttaaaag tgaaaaactc aaaggtcgca aagtaattat
     6361 gattggtgat ggaatcaatg attctcctgc tatttcagaa tcagatgtcg gaattgcaat
     6421 gagtgaaggt gctgaaatag caagagagat ttcagatatt accatatcag cagataattt
     6481 gaataattta atcgttttaa agcagattag taataagctt atgaaaagag ttgatttaag
     6541 ttctaagttt attattggct ttaatttagg tctgatttta cttggtgtag gtggatttgt
     6601 tagaccaagt acatctgcct ttttacacaa cacatctact gtcggaataa gtttaaacag
     6661 tatgacaaat ttattggaaa acaccaatac ttataactac cattaatctc cattacataa
     6721 aacatatgat ataaaaaatt tataattcat ctttttaaaa gaatatgtct taaaattagc
     6781 ttttttctaa tcaaaattat ataaagcata ctttacaaat tttaaactca acttatttct
     6841 attttttaca aaaaaggaga tgattcaaat tttcattttg aaacatcccc ttttgtaaat
     6901 ttctttaaaa tttatatatt aattatattt tcaaccaata attgtatttt caaccaatct
     6961 ttcttcatag cattttaaat cattttgctt tagataatta aattcagact ctacataaat
     7021 accacagcct accaattcaa ttgtcaaatt aggtatattt tcctttagta tactagtctg
     7081 ctcataaata attctttcca ataaatcaac ttgtaatata gaaaaatgct tcataatctc
     7141 cccatcacta tctatatctt tcttgatttc ataccaactc tttgacaaaa ttcccttcaa
     7201 atcaatatac cctagactcc tcaatatatc tatactatcg ctccatgcat acttactagg
     7261 tttatcaaag tatgattcta gtttcttctt ctgctcattt gtaagtgaat cataagaaag
     7321 cttcttatta aaatgtgcct tcaaagaaat cttagccata gaatatggac ttctaccaga
     7381 gtttaatcct gcatttttga acttttcaat tcctttatct tctactgaag accatattga
     7441 gtttattgaa tctgaatcat tgtatttaat atcaagacca tgtgtaatat ttatatagtt
     7501 ttgtggaaat acatattgat aatagacatc ttttttatca tcatatagag agaagtatcc
     7561 actatcatat aaatgaatat gcacctttac ttttaaacta tcctctaaaa tatttcgtct
     7621 aaggttcggg atatttgcat acatggttac atattttatg gcatcaaagt ttttatttaa
     7681 tggaacttca aaactaattt catctcctgt caaaagcttt ttcacttctt cttcattaca
     7741 atcatataga gaatatgtat tttcaaatcc cacacgtgat ttcccatcta aactgcctgt
     7801 taatgctacc ttcaaaatat ggttatctgt tttttgccat tggtaagcat cttggtcaag
     7861 aaactctcta atctgtctat tttgctcttt cgcctgattt ataacctcgt tttcatcttc
     7921 tgtaatccct acatggcatc tatttaagat atttactata ttgcttccaa atgggaatgt
     7981 ccatagttga aaagctgctg cctcaacctg acacaaatat ctaacctgat ttaattcaat
     8041 ttgatggata agttttttct gatattcatt catctgatta ccactatctg acatttgata
     8101 aatcaattct cccattgcaa gattatcttt tttcttatca atcttatcag taatgataat
     8161 ttgagtttcc atcatatcct gtactttttt aaatattata gaaatctcct ctgagttttc
     8221 catttttaag cttccaaact tcgtgcttaa ttcagttaaa acttccttca tctttaattt
     8281 acttatctca ttttcaaaaa tatcttgatt tttaactgta ctatcgaatt tttctacaaa
     8341 tggtataaca gattctttca aagttttatc tttaaattcc accatttgag tattcaattc
     8401 atctaatttc ttaatattat tttctttatc tttaattgcc tctttaacta cctctatttt
     8461 actctcaaaa ctagatttta cattaagaca attattaaca ccactaatcg cagtatttat
     8521 aagagaacaa gcagaacttc caatactcaa tgcattttta attttagatg tcttttgctg
     8581 tctttcaata gcttgtttac ttttctctag tgcagctaac ttatcttttt gctgtttatt
     8641 attgtaattt gcctccaaca tacctatttc ttttttctgg atttttgtgt aaatttcctt
     8701 atactcgctt tcacttaacc cctcttttgc tctttgaata tcgtcagctt tgtctgcata
     8761 gttatcatca tctatagact taactataat ctccttaatt tggtcaaatt tagttcttct
     8821 ttcttcatca acacctgcca aattataagt cattttatct aaagcatttt ttctatcttc
     8881 acttagctgt tctccaagta caaatctcat accatcagaa tccaatttaa aatctgaaaa
     8941 atcaaccatc ctgttagtcc attggtattc ctcttcttta gctgaaagat tttccttcaa
     9001 agattttgca ctgtcaaatg cccctagaca agcttgacca acaccactaa atgcagtcag
     9061 taaactcaat gcgctttgta ttccagaaag aattaactgt gttttcattt gttcctcaag
     9121 ttttgcctgt ttctttttta gttcctctat gtcttcttgt tcatcttctt tcatctttat
     9181 aatttcttga atcaacaggt tcatttttgc attaacagaa agcattttat tctcaagttc
     9241 attttttaaa acctctattt gttgatttcc agattcaatt tgagttagga tactttcctt
     9301 atatgtattg aaatatcctt cttttaactc atctttcttt ccttgttcca attctcttaa
     9361 ggtcttatcc agatagtctg tcaaagaacg aatatctgta acacgatttc ctccaatttc
     9421 actttcttca atcatctgca cataaaatgc tgtcttctgc tcaagtgcaa ataaaatcat
     9481 actccattcg ctaaaattct gattttttcc tacaaaagat tctattctat cagactgcat
     9541 caatttattt ataaggtatt catagatttc ctttgcctct ttataaaatt ctttctttat
     9601 attcatgtca tccatagaaa gtactgcttt atttttcttc tcaaaactat agtacttctg
     9661 aagtatttct ctatttaatt gcatctctat tttctcaact gcataaaaaa tttcataaat
     9721 atgtgaaaat tctaaaatat cagcttctaa aattcctact gcacacaaca gtctaagatt
     9781 tctataacct ttaattccag aaaaaccaaa tctctcgaaa aattcataga agaatgtatc
     9841 tattgacaaa gaatactcct gctttaacaa ttccttttct tttagtgcat ctccagagtg
     9901 accggctttt ccctctgctc cggctgaacc tgaatcttca cgtttgctaa ttgtccagtc
     9961 cttatgttct tcataaagag tatttaattt ctctccatca tctgctgctt gcttctgatt
    10021 taaagcatga ttaaacacat atctatcaaa ataatagtag tttctatcac tattatccat
    10081 tggaggagag gtacctatat atcttattgt ttcttcatat atacctctaa ttccaccaag
    10141 acctcctgca ccaccagctc ctccagagcc tccttttcct gctaaaccat gatttccatc
    10201 ttttttactt atagcacttt ttaataaagg agcttttcca aggagataaa cctgtccaga
    10261 cttgcttcca agacctgcat ttccaccatt acctcctgtg cctccagttc caccttgttt
    10321 tcctttttct gcttttacaa catagtattt tgtaccttta acattagcaa attcttttga
    10381 ctctttttta gaacctattt tagcatttgc cccagcttct ccagttatcc ctgtatctcc
    10441 tttttttcca tcttgtccat ttccaccacg tccaccatct acgtaaagtt taagttctcc
    10501 tcttttttct atttctttaa aagataatat gatatcacct gaagtacctg cattattgcc
    10561 atttcttcca ttcattccgt cgtaaataga actttctcca accaattttt ctgcatcctt
    10621 accagatacg ttgattgaat aagtcccttc tggtatatgt aatttttctg taagtagtac
    10681 aatattctta cctgagaaga cttctttctc catattacag tcaattttca actcacaatc
    10741 tccatacata tatatagatg acaaaatatt aagttgataa ccagatatta ttttagtcaa
    10801 atccgataaa ttcactttag gtataggaat atatatagaa agtacatttt ctttctcata
    10861 tgattttata tatgtacaac tttgaatctt ctctatccta tactctgcat cttcaagctt
    10921 tttggcttct tcttccctat attcttcttc tgctatccaa tatttgatat cttttaatgt
    10981 atcttctaaa gtatttttta gcaatgattt taacaactca cttagaccaa caatatgagt
    11041 ctcactacag taactattta actctaaaag aagacgtgtt tcttctaata tattcacttt
    11101 ctgtacgcca tcaccatatg aaagttcttg tacaaactcc ataagctctt tcatattatt
    11161 agtcctacta cataaatctg tatacatctt atctaattga atctttttat cttttagact
    11221 atttatagaa gatagaatgg aaatagaatc ttccatacct tttttaacac tttctatata
    11281 aggaactaaa agtttttcta ggtctttata ggttacatca acagcttttt gtatttctat
    11341 tttacaagaa gctgtaagca tcaatcccaa atctgttttt tcaaatgttt caggtagatt
    11401 tttcatttca tccaacaact tttctggctg tttatctagt ggattacata atttaataga
    11461 tacaccatct gataatgcta catttaataa gtacactaat tccttacaac cgtcgtaatc
    11521 tttctcttga ctatctaagt cttcttttgc tttttccaat atatttctta ttttttcttc
    11581 tgttacatta ccgattagtg ttttaggtat tttattaata agaaagaaga aattttcttt
    11641 atactttgca taatcaccaa gcatatcttt caaatttttg cctgtttcta tcaataatgt
    11701 atgtctagag tccagtaggg attgatagtc taagataact accatccctt tcacctttga
    11761 agatgtggaa atagtttccc ttacaagcat agcaccagca atcatatact ctgctccacg
    11821 gttgtcaaaa aaacctgggc aatcacataa tgaaaaatct ctatttgcaa tatctacaac
    11881 ttctggacat aaagtcttag aatatacacc atttccaatt tcgacagctg aatcttttgc
    11941 agttatatat atttgtccag tctcttcatc cgttttttct tccatcacac agccttttaa
    12001 ataatttata caagtactct ttccaacgcc tgaagcacct aggaataaca aaattgactt
    12061 ttcttttaag tcatattttc taagctcgtc tcttccttct tccaaaaggt ttacgatttc
    12121 actataaagt ttatttgcca tacatccatc cctttctata tttcggaaca atagccttga
    12181 tatttttctt taataagatt ctcttctacc tttgaaacag ctgttgataa cttagtattt
    12241 ggagcattaa atcctccatt gtctcctcta tctccatatt cagcgtattc tcttgctggt
    12301 cctgttttac cttcattcca gcttccacca ctaaattcta tatgatattt aattatacta
    12361 tctaacttat aatcccaact tgtaaaatat cctgtatagt atacagtctt atctatacca
    12421 cttggagaaa atgaaagtgt attatatccg tgatagccac cacttcctcc atatccacct
    12481 aaacctggtc taccatttgg acctgcttct ccatctttca ctgttatatc acaatgatta
    12541 attattctat ttttttgttc ctctgaatct gaaatgaagt aaatattacc tttcttacca
    12601 gcatttccac ctcttccagc atttccacca gctccacctt tttcaccttt tttgccactt
    12661 gtaccttttt caaaataccc tttaaacatc tgtccgtgga aataacttgt atatcctgca
    12721 tcagctccat tacttccaga agctcctgtt tcaccatttt ctccatctat tcctttagaa
    12781 ccatttcctc catttgcaat cagtgttaga cgataatttc taatttcttc tttaatatat
    12841 aagtaaatat tacctgcatc tctaccatct tcaccatttt tcccatacat agaattatct
    12901 acaagttctc cgctctttcc acttacatta atttttacat tactacctac atcaagattg
    12961 ttagaagcaa gaataatatt tttctctgat gcaaagttca ttccaaggtt atcatccata
    13021 taaatagtat ctttagcaaa gaacataaaa gtagaaacat tgtcatctac ttttctattt
    13081 ttgaaatatt ccaatacact gctaattact aagtttggaa ttaatgctct tatttcaata
    13141 cctttcttat cattaatatt aattgttttt actataaatg gatttaaaat gtatttacca
    13201 ataaattctc taattatctc attaccttct tctccatatg cacttaatct tgttatactg
    13261 tcttctaact cttcattttg tgtaactgta acatttgtca aaattgaatt aaaatcactt
    13321 acactatata cataatcctt tttatttgca acttccatta atttttctct atttggtaat
    13381 gtaaatacct gtttttgcac ttcataagaa actaaaactt tttctaaatt ctttcttatt
    13441 ataatgaagt tttgttccat tatcttctta cttgcctttt ctataataaa ttccatatgt
    13501 acagaaggta ataaaatttc cccagttcca tgttctgtat atttacttaa agcatcaatt
    13561 atatctaagt ttccttttat cttattttgt aatttcatgc aactatcttg taagaataat
    13621 ccactttgtt tggctaatac gttgataaaa ctctctatag taagacttgt tgtactttcc
    13681 aaccagtttt taaatacttt tacaacctca tcatattttg ctatttcttg aatcattgtt
    13741 atacttccaa taatattgat aaaatcttct gtatacgcct taacataatc ttctaggcta
    13801 gatacgatat tgttttgaat ttcttttatc aacgtatcta aaactaatct tgcatctgca
    13861 ctaattggat attggaatgc tgatgtctta tctttaactt catctaattc tagtacttgc
    13921 tttaataagc cttccctctc atctgatgca agtggcttac aaagatgaat attttttcca
    13981 ttatctttta gcaattcttc aaataaccaa gctttttcct ctatattact gtctaaattt
    14041 tcgcataatt gttcatattt tctttttata acattatata tttgtttttc tgttcttcct
    14101 aagaaacttt ttgaaatgaa aaaagttata ctacccttaa aattttcttt atgaagacac
    14161 atatttaact gttggaacag gtctagtact tttgtaagtc tagaatctaa tatatctgct
    14221 tctggtatca atacaattac tgcacatata gatgcagaat ttttaaatac ttcacctaat
    14281 gaaactgctg cactaatatc atgggctact cccctattgt cgctaaaacc tgcagtatca
    14341 cataattttg catccattcc ttctattggt tttctcatat ttacaatgtt cgggtataaa
    14401 gtttgagaaa cagctcctga accaatctct gccacagggt ctaagcaatc aatatatgat
    14461 ataccagtat cttcatcttc tacttccatc attacacatc ctgcaagata gttaattgca
    14521 gtacttttgc cacatcctgt cccaccaaga attagaatta aattttgatt ttcagcctta
    14581 tttattgcta cttttccttc atttaataaa agtgttattt cttctaaaag ttgattgtta
    14641 ttttctggca tttattttcc cccttggtta tatattttat ttaaataaag tttaatccct
    14701 taagtagatt gcaaagtttt tagactatat ttaaatttgt tttttatttt ttatttttaa
    14761 taaatttgca ttttcactaa ataattattc ttctttgttt attaaaataa ctgtgttgta
    14821 taatataatc tttatcaaca cagttatttt atttaatgcg cttattttat tgtattttat
    14881 gtaatacagt tgttttttat attatatgtc cattattttt ctatggttat tacatataga
    14941 tttttaatta tatttgtacc attaatttaa acattcctat actactgctt aagttttaca
    15001 cctataccag cttgaatatt ttatttttat ttgcttaaat agttcaacta tactggttta
    15061 aataggttat ctttatttgc ttaaatagtt caactatact ggttaaatag gttatcttta
    15121 tttgcttaaa tagttcaact atactgattt aaataggtta tctttatttg cttaaataag
    15181 ttcaactata ctgatttaaa taggtttcct ttagtttaaa taattcacct atactggttt
    15241 aaataggttt cctttagttt aaataattca cctataccta cttgaatagt tcacttttat
    15301 ttgcttaaat tctataccaa tactggctta aacattatac taccatgatt gtattgtcta
    15361 tcttcaacta caataaaaac aggttctaaa tctttagtag atgttatttt taattctaaa
    15421 aaaatattat cagttggtaa tatttttaca tcataattat ttaaattatt attcgtgata
    15481 ctattaaata catcaccttg acgttcaaaa aaaacattat caactattac aaaataaaca
    15541 tcttcttttc tactattttc aacctctata gtattttgtt tagttatatt tagtctcatc
    15601 ttccacctcc tattaatatt acttaattat attaatactt gtaattgttg cattatgttc
    15661 atcacaagga agaggtttaa aaacaactat ctctgctaaa taattctgtg gaggatttgt
    15721 aactgaagta gcattgcaaa taaatttaaa atttgttcct gctacaactt gagtagaaac
    15781 aataagtggt tcataaccaa ccccttctaa cattcctaca gcttcattaa atacttttaa
    15841 atcttcttct tcgacagctc tgaattttga ccatccacca acaaaaagac tcatataaaa
    15901 acctccttta tatatatatt tatttatata tagtagtacc aattttctca atattcaata
    15961 ttttctgtgt aactggtcaa tgaaaatgca taactggata aaaatactta taaaataact
    16021 taactttaca tataaatagc ttactttaca tgtaataaga ttattctaca tgtaataaga
    16081 ttattctaca atatgaaatg catcttgttt tgaatatatt ctaaatacaa taatggatat
    16141 tttcaattat tataattaga aaatatccat tacttaactt gttttattta aaatcacata
    16201 gagactttat tatttttttt attgcatgaa tagctatttc aggtatcata aggtgatata
    16261 ccccctcctt gaaaccacaa aaaacatgac aaactttacg aatcttgtct taaaaaacta
    16321 caggtacatt ccttatttgt ggattggagt agccataaca agcaaatttt acaattttta
    16381 tgatgacaaa aatcaataaa atcgcttagt atataacacg aaatactact tgttactatt
    16441 aagaatagtt agataggaag tatgcattat acaaataaag gaatcaataa agaaagttaa
    16501 aacatcccta caaattaaaa atctgttaca tttttctata tttaaacgtt atttacttac
    16561 aattggagaa ataacaggtt ttccttctcg gtcaagtaaa ggcgtaaatc cggctgcatt
    16621 gccataatag tggaatacat agttcacacc tgtttcttta tccacccaaa tctccgttgc
    16681 atttattttc ccttgtgaat atactttttc aaatctatct tttcccattt tataatcctc
    16741 catatttttg attggtctat gccttcttca cttaagtaga aaaaatacat aacttatttt
    16801 tttaattcta atatgagcac gtaaaaatgt caaacagctt atttaatttc ataacttcat
    16861 gttactgttc gtaaccttgt ctgtttactt acactgcatt acagcaccat tttatccttt
    16921 acattattgt attgcacaca gtaaaaatgc ttactttatg ggatttttca actaaaaaaa
    16981 ggattttatc tccacaaaac atttactatt tgttacaata tacctaattt taatctcata
    17041 tcaacattta atgaagatat aacctttttc aaatattgta aagactcctt aaaaatatta
    17101 gaaaaagatt ctgcaaatct ctcaacatct ccttttttta aatacctaag tatctcaaaa
    17161 catacatcaa aacgtgtttt caaaatttca ttatattctt catgaaaaaa tctctctaaa
    17221 tagattccaa atatcatata ttgttttaaa tgcaaaaatg tttcctgtaa tacataaatt
    17281 tgtgtataat ctactatctt acaaagtaaa acaaaatctg gataaacctt acactcttca
    17341 caattatttt gtaagtacct taaatgagtt tctattttat ctacagctat caaatccatt
    17401 ttatttccta gtaaaagtac tacatcctca agagtaatcg taacaaactc catagcatcc
    17461 aaaagcaaac tcaacctttt agctccttct tctgtattta tataatcatc atcatattca
    17521 gaaaaaagcg ttacatatgt tcccttgcca ttaattgttt tagcaattcc caattcattt
    17581 agctcattat aggcacttct aactgttgga agtgatacat tatatctatt aattatcgaa
    17641 gtaattgatg gtagacaatc tcctatttta atattgcctt caaaaatttg acaaattaca
    17701 tctgaaacaa ttaagtcata taaataacat ttttccaacg ttacagaatt aaaactactt
    17761 gtatctatac tttgtggagc acgattttct accattttaa gtatggattc tctacaagct
    17821 ttatacatat atgttaatag ttttttaaat tcaattagat tcccatccaa tccatataag
    17881 taagtttttc ttacctgctc tatgtattta ttacgaaaaa agtctataca acaattatcc
    17941 aagttatcca agttatcgaa attataggcc aattgtgaaa taatgacatc atgctgaata
    18001 atctcacaaa aatggtgaat caggaaatta tttgttttac ttattagttt atttatatat
    18061 tttagccttt gttctttaaa gactaaataa ttagaactat ttttttccat acaatcgata
    18121 atctcgttta attcttcaaa gtctttagat gtaaatcttt taatattgta catagcaata
    18181 gaaggaaata ttaaactaaa aatcttatat atttctgata tttgtaaaat cttctcatat
    18241 aataagtcag attctattga ttgtttactc gtactctgga ttaaaactgg tctagaacca
    18301 gcttttgtct ctataaagcc cttttctgag agcattttta ttacagtttt tatagtattt
    18361 tttgatacca tatattcttc tgacatgctt tttatagaat caagcctatc tccaatagta
    18421 attttaccag tataaatttg ttgaattata tgctcaaaaa taattaaata ttgtggtttt
    18481 ctcttcatat ttttcctctt cgataaatta gatttattgt tttttgtaag gtatatttac
    18541 ttttatttaa tagggcaaaa tagttgctta ttttttcctc ataatctata attaatataa
    18601 accatttata tatatggtct aagttttata atactgtaat gcagtaaaat agcatgttgt
    18661 agctatacta cagcaaatta acataccatt gtagtatatt aattggaata attttaagta
    18721 cttcaaaatt ccagcaaaac atatatgtgt ttttgatatt atgtatattc gttaatagaa
    18781 tagctatact tgttttacat aataaattat atgatacttg ctatatgtaa tcactatatt
    18841 taatacacat aactattata cttgctatat gtaattacta tactttatac acatagttat
    18901 catatatgct atatgtaatt actatacttt atacacatag ttatcatata tactatatat
    18961 aattactata cttggacaca tagttatcat acatgctata tgtaattact acacttggac
    19021 acataactac tatatttact atatgtaatt atactatact tggacatata gttatcatac
    19081 atactatatg taattactat acttgaacac ataactatta tatttgctat atgtaattat
    19141 actatacttg gacatatagt tatcatacat actatatgta attactatac ttatacacat
    19201 aattatcata catgctatat gtaattacta tacttataca cataactatc atacatgcta
    19261 tatgtaatta ctatacttat acacataact attatacttt ccatacataa ttactatatt
    19321 tggacacata gttatcatac atactgtata ttaagatata atacttttta aattttatat
    19381 aacacagaca catgcaataa aaaagaaggt atccataata agctctttgc atgtacctaa
    19441 ataaaaactg taaaatagaa tatataaaat ctaatctatt ttaagtaaat acaatttcac
    19501 tttaaaagag gatgtactat gaaataccat atatttttaa tcataactac tatattaata
    19561 atcgaactat gtaacattta catatgcaaa atagcatctt ttggaaacaa aaatataact
    19621 atacataaga attacaaata tattttttta ataaaaccat taaccttctt gataataata
    19681 ttcacaaaat taccattacc actatattat atattgtttt acatagtaag tcataaattg
    19741 ctcttaagca gaaaaagttc tactaacaac cattttattc ccaatatggc agtaataagt
    19801 ttttgtattt tacaccttat tactgtaagt acaatatcac ttgttaaaaa tacaagtcca
    19861 gatataatat ttgcaaatat gacctataag ttaattagta ttttaatagc ttctatttta
    19921 gattttatct tatggtactc tatgaataaa ataaaagagc atatttatat aataactcaa
    19981 aatcaaatag agttcagtct atttttaaaa tttatctggt tcactttagg atatatatta
    20041 tttgatagta tacaaattac atttgctcca ccaagttctc taacttccta ctttatattg
    20101 ggaagtaaca tattattaat cttacaacta attatattca tcaatcatat atattccatt
    20161 gaaaaaaata tttatttaga aaaagaacat aatagactaa aaatgataaa acaagaacaa
    20221 ttactaaaag taaatttatt aaagaaaaaa gctaacattg atgaattaac atcagcatat
    20281 aatcgaaaat ttgcttttga aaaaatacag cattacattg ataacaattt attattttca
    20341 ttgatatata tcgatttgga taaattaaag acgataaatg atacatatgg acatttagct
    20401 ggggataact atattattaa cttctcaagt atagttattg aaaacttagg aatagatgac
    20461 atatttgcta gaataggagg agatgagttt atagttatta tgcctaaatc taatgaaaac
    20521 accacaaaag aatttataga gaaaataagt aaaattatgg ataatacgat atcttttagc
    20581 tatggtattg tagaagtttc tagcgaaaac aaaatgactg tagaagaaat tattaatatt
    20641 gctgatatta gaatgtacac taataaacaa aataatcata aaaggaacaa taactaaaat
    20701 gaatatttac atgagcttac ttgtagtctc actatgtttt ttatcttatt atctctatca
    20761 ttataatttt tttaaatatc tggacataat gattaataac tctaaa
//
LOCUS       DAYASV010000065        20735 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYASV010000065 DAYASV010000000
VERSION     DAYASV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.065"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI68_002237"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="ACGI68_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFH0138672.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="ACGI68_002238"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="ACGI68_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFH0138673.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI68_002239"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="ACGI68_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFH0138674.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI68_002240"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="ACGI68_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFH0138675.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI68_002241"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="ACGI68_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFH0138676.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI68_002242"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="ACGI68_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFH0138677.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI68_002243"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="ACGI68_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFH0138678.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI68_002244"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="ACGI68_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFH0138679.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI68_002245"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="ACGI68_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFH0138680.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI68_002246"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="ACGI68_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFH0138681.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI68_002247"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="ACGI68_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFH0138682.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI68_002248"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="ACGI68_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFH0138683.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI68_002249"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="ACGI68_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFH0138684.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI68_002250"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="ACGI68_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFH0138685.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="ACGI68_002251"
     CDS             6989..7174
                     /locus_tag="ACGI68_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFH0138686.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI68_002252"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="ACGI68_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFH0138687.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI68_002253"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="ACGI68_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFH0138688.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI68_002254"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="ACGI68_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFH0138689.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI68_002255"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="ACGI68_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFH0138690.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI68_002256"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="ACGI68_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFH0138691.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI68_002257"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="ACGI68_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFH0138692.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="ACGI68_002258"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="ACGI68_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFH0138693.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="ACGI68_002259"
     CDS             11117..11767
                     /locus_tag="ACGI68_002259"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFH0138694.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="ACGI68_002260"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="ACGI68_002260"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFH0138695.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="ACGI68_002261"
     CDS             12536..12805
                     /locus_tag="ACGI68_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HFH0138696.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="ACGI68_002262"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="ACGI68_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFH0138697.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI68_002263"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="ACGI68_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFH0138698.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI68_002264"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="ACGI68_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFH0138699.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI68_002265"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="ACGI68_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFH0138700.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI68_002266"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="ACGI68_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFH0138701.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="ACGI68_002267"
     CDS             14901..15848
                     /locus_tag="ACGI68_002267"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFH0138702.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI68_002268"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="ACGI68_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFH0138703.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="ACGI68_002269"
     CDS             16330..17163
                     /locus_tag="ACGI68_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFH0138704.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="ACGI68_002270"
     CDS             17151..18017
                     /locus_tag="ACGI68_002270"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HFH0138705.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="ACGI68_002271"
     CDS             18011..18814
                     /locus_tag="ACGI68_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFH0138706.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="ACGI68_002272"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="ACGI68_002272"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFH0138707.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI68_002273"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="ACGI68_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFH0138708.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI68_002274"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="ACGI68_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFH0138709.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8282 a         2448 c         4322 g         5683 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc tagatgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tatcccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaa
//
LOCUS       DAYASV010000066        20544 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYASV010000066 DAYASV010000000
VERSION     DAYASV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.066"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            62..3010
                     /locus_tag="ACGI68_002275"
     CDS             62..3010
                     /locus_tag="ACGI68_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138710.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            2988..3452
                     /locus_tag="ACGI68_002276"
     CDS             2988..3452
                     /locus_tag="ACGI68_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138711.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            3565..3867
                     /locus_tag="ACGI68_002277"
     CDS             3565..3867
                     /locus_tag="ACGI68_002277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138712.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            3944..5293
                     /locus_tag="ACGI68_002278"
     CDS             3944..5293
                     /locus_tag="ACGI68_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0138713.1"
                     /translation="MEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGLTVGVG
                     FIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALIIPIGL
                     VVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMVIVLVMAD
                     LTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQKKFGL
                     FGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLPVSEAA
                     QEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVLPFADL
                     AVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFKMPEGA
                     TMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKLSIEE"
     gene            5328..5801
                     /locus_tag="ACGI68_002279"
     CDS             5328..5801
                     /locus_tag="ACGI68_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138714.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINDGKLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            5973..6623
                     /locus_tag="ACGI68_002280"
     CDS             5973..6623
                     /locus_tag="ACGI68_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFH0138715.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            6797..7729
                     /locus_tag="ACGI68_002281"
     CDS             6797..7729
                     /locus_tag="ACGI68_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HFH0138716.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYYDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            8003..8716
                     /gene="ispD"
                     /locus_tag="ACGI68_002282"
     CDS             8003..8716
                     /gene="ispD"
                     /locus_tag="ACGI68_002282"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFH0138717.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            8713..9198
                     /gene="ispF"
                     /locus_tag="ACGI68_002283"
     CDS             8713..9198
                     /gene="ispF"
                     /locus_tag="ACGI68_002283"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFH0138718.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    9293..9501
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            9565..11280
                     /locus_tag="ACGI68_002284"
     CDS             9565..11280
                     /locus_tag="ACGI68_002284"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0138719.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFNRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            11402..12847
                     /gene="proS"
                     /locus_tag="ACGI68_002285"
     CDS             11402..12847
                     /gene="proS"
                     /locus_tag="ACGI68_002285"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0138720.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            13285..14766
                     /gene="gltX"
                     /locus_tag="ACGI68_002286"
     CDS             13285..14766
                     /gene="gltX"
                     /locus_tag="ACGI68_002286"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFH0138721.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            14987..15142
                     /locus_tag="ACGI68_002287"
     CDS             14987..15142
                     /locus_tag="ACGI68_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138722.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            15287..16684
                     /gene="cysS"
                     /locus_tag="ACGI68_002288"
     CDS             15287..16684
                     /gene="cysS"
                     /locus_tag="ACGI68_002288"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFH0138723.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            16687..17082
                     /locus_tag="ACGI68_002289"
     CDS             16687..17082
                     /locus_tag="ACGI68_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HFH0138724.1"
                     /translation="MEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRKVNDLHK
                     LAIKYVKAKAQATIIHEVEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKHATGFEA
                     LVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            17188..17946
                     /gene="thyX"
                     /locus_tag="ACGI68_002290"
     CDS             17188..17946
                     /gene="thyX"
                     /locus_tag="ACGI68_002290"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HFH0138725.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            17959..18696
                     /gene="rlmB"
                     /locus_tag="ACGI68_002291"
     CDS             17959..18696
                     /gene="rlmB"
                     /locus_tag="ACGI68_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFH0138726.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            18699..19226
                     /locus_tag="ACGI68_002292"
     CDS             18699..19226
                     /locus_tag="ACGI68_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFH0138727.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            19291..19938
                     /gene="sigH"
                     /locus_tag="ACGI68_002293"
     CDS             19291..19938
                     /gene="sigH"
                     /locus_tag="ACGI68_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HFH0138728.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            20012..>20544
                     /locus_tag="ACGI68_002294"
     CDS             20012..>20544
                     /locus_tag="ACGI68_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFH0138729.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSAL"
BASE COUNT         8496 a         2149 c         3848 g         6051 t
ORIGIN      
        1 aaaaaaaaca atatatggca tataagttgc tatataaata ataaggaggg aacaattata
       61 aatgaggcaa agaataaaaa acttaattga aaatgaagat aagaaaaatc ctttaacaga
      121 tgaagcgatt tcaagtaggt taaatattcg aagagaagat gttacctttt tgagaaatga
      181 gttaaaaata gaagattcaa gacaaagaag aaaagttgtt ttaattaaag ctataaaaga
      241 tattttaaaa gaagaaaaaa ctataaataa aaatgatata acaagaagat taaacagcat
      301 tgggtttaca gtatcaagat ttacagtcat acaatactta aaagagattc aagattctgg
      361 tgacctaaaa cttgataaag agagtgatga taaaaaagaa gaaataatca ataatagaga
      421 aaataaagat atagcatttg ataaactcat aggtagtagt ggtagcttaa gtacaattat
      481 aaaactagcc aaagcagcaa ttctttatcc acctcatggt cttcatacta ttattatggg
      541 gccaactggt gttggaaaga gtgagttagc agagtgtatg tataaatttg cagtagaatc
      601 atccagattc cctaagaatg caccatttat agtttttaat gcagcagatt attcagagaa
      661 tccacagctt ttgctatcac agttatttgg acacgttaaa ggtgctttta caggagctga
      721 tgagaacaaa gaaggattag tatcaaaggc agatggagga atattgttta tagatgaaat
      781 acatagatta cctcatgaag gtcaagaaat actatttcaa ctgattgata aaggtatggt
      841 cagaaagtta ggagaaacaa gactaacaca taaaatagat gtaatgatta tttcagcaac
      901 ttctgagcca atagattctc atttacttaa tacatttaaa agacgtatac ctgtaacaat
      961 tgaaatacca gaattagttg caagaccatt aaatgagagg tttgacatta taaataattt
     1021 cttcttaata gaagctcaaa gaatgggagc aaacatacat ataaagtcag atgtattaaa
     1081 agctcttatg ctttacgatt gtataggtaa tgtaggtcaa cttagaagtg atattcaagt
     1141 agcttgtgct aggggactat taaaccaact tacaaacaaa ctaaaagaag taaatataag
     1201 catatcagat gtacctggat atgtaaaaca aggtcttata aaaatcagaa attgtagagg
     1261 aaaaatagaa aattatgtag atggggattt aattatagac tcaaatcttc aaggtgagat
     1321 agaaaagaaa aatgaagata tatatacttt tccagatgaa atatacaaac ttactgaaag
     1381 aagacatatt gatttattgg aacaaggttt agattttgat gttataaata gaatcattgg
     1441 aggagaatta gaaagttgca ttcaaaaata cataaaacaa gttaaaaaag tttctattag
     1501 taacaatctt ccagatataa gtccaattgt tggaaaatat atcgtagagc taacttatga
     1561 agtaatcaaa atagcatcac aatacctaag ttcactagaa ccgaatctac atttatgttt
     1621 agcagttcac ctaaaagcag cttttgagag attaaaagaa ggaaaagtaa tatcaaatgc
     1681 tcatttgaaa gaaataatag gaaattatcc actagaatat tctgtagcag aaaaggtagc
     1741 tcaatattta aaggaaagat ataacataaa cttaccaaaa gaagaaatag gtataatagc
     1801 aatatatttt aaaatgagta gtagaagaga tatgtctgaa caaaaaaaga taggtgtact
     1861 tcttatggct cacggaagag tagcatcttc tgtatgtgag gttacaaata aactattagg
     1921 aattaatcac gcaaggtatt tagatatgcc atttgagaag aaaccggaag aaatgattga
     1981 agaagctact acaataataa aagatattga tgaaggtaga ggtgtgatta tattagtaga
     2041 tatgggttca ctttcatatt ttggagatat tatttctgga aagactggaa ttaatataag
     2101 gacaatatct agatttgata cattgctttc tatagaggtt atacgaagag cagttttacc
     2161 agatttaaca atagacgaga tagttgatga agttaaaggt atagagccac aagtaaaaga
     2221 aaaggttagt aaatgtaata atacaaaagt agattttaga aaacctataa ttttaacatt
     2281 atgtattact ggattagggg gagcttgtaa gataaaaaaa atcctagagg aaaatattaa
     2341 aagtataact gaaaaggttg atatagaacc tataggtatg attgaatatc caagtcttga
     2401 tttagagata aaacaaataa gcaatgataa aaatgtaatc gccatagttg gaacaattga
     2461 ccctcagata gcaaatatcc ctttttttag tctagtagac ataaatgata aagcaaagtt
     2521 aaatcaaata aataacctaa ttgatacaaa tcaaattggt gaaacaaata aaaaatatga
     2581 attatcagat ttaatacata aagaagatat atttgtggat ttacaggtta aatcaaaaga
     2641 agaagttcta aaatatatga gtaaaaaact tagagagaaa ggttatgtga caaaaaattt
     2701 ctataaaaaa gtagtggaaa gagataactt atttccaact gaattatcag atgaaatagc
     2761 aatacctcac acagatggaa tagatgtaat taggccagct ataagtatag ccatcttaaa
     2821 gaagcctatt gtgtggagta aaagtaaagt aaaaataata cttttattag ctgtagacca
     2881 aaagtgtttt aatccattaa gcactctact ctcatttata gaaacagatg aatttaagaa
     2941 gataaaaaac attaaagata gagattttat tagggagatg atattagatg gagtcaatga
     3001 aaataattag tagcaattta atttttaaaa atattgaagt gtctaacaat gaagatgcat
     3061 taaagttttt gggacaacgt ttgtttgacg aacagtatgt aaaagaaagc tacatacaag
     3121 ctgttgtagc aagagaaaaa aagtatgcta cagggttacc aacagaaata tacggagtgg
     3181 caataccaca tacagatata gtacatgtaa atgagccagg aatagcaatt ggaatactga
     3241 ataaacctgt aaaatttata atgatgggta cagacgatac tgaaattgat gtaaaagttg
     3301 tatttatgtt agcagtgaaa gagccacaag aacaattgca actattagaa aaacttatga
     3361 ctatatttca agataagaat atactaaata atattgttga cttaagtgaa gaaagtgtga
     3421 gtgatttatt aaactctaaa ttgaaaaatt aaacatgtaa agtatattaa aaatgtaaaa
     3481 ttaaacatgc aaagtatgtt aaaaaatata aaaagattat taaagatttc taactataga
     3541 tatctttaaa ataaaataat ttcaatgggg gtgactaatg tggctaaaaa gatattagtt
     3601 gcatgtggaa caggagtatg tacttcaaca attgctataa acaaattaaa gaaagcactt
     3661 caagacattg gtaaattgga tatggttaat attacacaat gtaaagtagt agaagttgca
     3721 tcaaaagcac ctgattatga tttaataatt tgtactacac aagtatctag tagtataaaa
     3781 acaccagtga taaatggatt acctttttta actggtgtcg gaatggataa attaatcaat
     3841 gatgtattag aagagttaga attataatta aaaaaattaa ggaacatggt ctctaaaatc
     3901 ataagtttta gagacttaaa attaaataat ggagggtctt tatatggaga tattaaattt
     3961 cattgttggt ctaggtgcac aagtaatgat gccaataata atatgtattt ttggtttgat
     4021 attaggtaca aaactaggaa aatctttaag agctggttta actgtgggag ttgggtttat
     4081 aggtcttaac actattatag ctttgctaac agataattta ggtccagcta cacaacaaat
     4141 ggttaagaat ttagggctaa gtttatcaat aatagatgta ggatggccag cagcatcagc
     4201 tatagctttt gcatcaacag taggggcttt aattattcca ataggtcttg tggtaaatat
     4261 agttatgcta attaccaata ctactcaaac tgttgatgta gacatatggg attactggca
     4321 ttttgctttt acaggagctt tagtggcagg ggctactcaa agtgtaatgt ggggagtatt
     4381 tgcagcagta gcaaatatgg taattgtatt ggtaatggca gatttaactg ctccaggtat
     4441 agaggaatat ttaggtatgc caggtatatc tcttcctcat ggatttacac aggcatttgt
     4501 tccaattgct atagtagtaa ataaattatt agacttaata ccaggcataa ataagattga
     4561 gataaatgct gatacattgc agaaaaaatt tggattattt ggagaacctt taataatggg
     4621 gtctgtaata ggtgtaataa ttggtattgc tgcgaaatat gatataaaag gaatattaca
     4681 attaggtgta actatgggag ctgtattaat attaattcca aagatggcag cattattaat
     4741 ggaaggatta ttaccagtat cagaagctgc acaggaattt attgagaaga gatttaaaaa
     4801 cagaggcaag atttatatag gcttagattc agcagtagga ataggtcatc ctgttacatt
     4861 atcagttgca ctagtgttgg ttcctttgac tatacttata gcagctatat tgccaggaaa
     4921 taaagtttta ccatttgcag atttagcagt aattccattt gcacttgtat taatcgtacc
     4981 aattacaaag ggaaatgtgt ttagaacatt aataatagga ataattataa ttacttctgg
     5041 attattaatc gcaactaact tagctccatt atttacccaa atggctttaa atgctagttt
     5101 taaaatgcct gaaggagcaa ctatgatttc aagtatttgt gatggtgcga atccattaag
     5161 ttgggtattt gtaaaagtta tgaactataa agttattgga ggagtagttt ttggagttat
     5221 agctttagga atggctattt acaataggaa tagaataata aatgaaaata aaaagctatc
     5281 tatagaagaa taaggttaaa acaattttat taaggagtgt ataggttatg aaaaaatata
     5341 aatatgattc taaaagatac ttactcagtg ttacaatacc aggatttttt atgattttaa
     5401 tattgattta tgcactgttt aataactata caaattttaa tttgaatata tatagtttgt
     5461 taataattgt ctgtacttat ggtatattca atacttttat atcatcaagt aatcctaaaa
     5521 aaattattat tgatagcaaa tgtatacatt ttacctcatt tatgactaca cataagtatg
     5581 aaattaaaga tatagaaaag ataagaataa gagaatttta taataaaaaa atttatctaa
     5641 aaattaatga tggaaaactt ttcaaaggta ggtattggat aagaacaaat atgtttaata
     5701 atagtatagc attatatagc tatttcattg agttggaaga gtgcttatat ccagattcac
     5761 taaaatttag aaataaaaaa tttgagaata agaacatata aaaataaaaa attgaattga
     5821 agcctagctt aatctttttt gataagatat aagtttgaac tttatactaa atttttagaa
     5881 ttttcaatca agacaataac ataatagcta agcaattaga acacaactaa gatgtataag
     5941 gcatgtatat gatgaatggg aggatttaat taatgttatt agagaaatta agaaaagaag
     6001 tattacaagc atctttagac cttttaaact ataatttagt gacattaaca ggaggaaatg
     6061 taagtgggag agatgaacaa actggctata tagcaatcac cccaagtgga atggattata
     6121 gaaatcttac accaagtgat attgttatag tagatgtaga tggaaatatt atagatggaa
     6181 aatggaaagc atcagtggat ttatcagacc acttgtatat atataagcat agagaagaca
     6241 taaatagtat aattcataca cattctacat actcaagttg ttttgctatt ttaaatgaac
     6301 ctatagaatg tgcaagtaca actcttgcaa atgaagtagg gggttcagta ccagttgcaa
     6361 aattttcgcc accaacatct aaaaagatgg gaaaatgtgt tattgaagca attggtgaca
     6421 aaagagcatg tcttttagca aatcatggcg taattgcagt tggtccatct gtaggacatg
     6481 ctcttacagc cgctgtaatg ttagaagatt cagcaaaagt atattactta gcaaaatcaa
     6541 ttggcacacc agtgctactg cctgatgaag aaattcaaag agcgagagat gtatttttta
     6601 atgtctatgg tcaggataaa taaattagat aaaattttaa aagtaatatg aaaatataga
     6661 tattcaatga tattaaggag atttataatg atataataag tttaatagtt aagaagtaaa
     6721 atatgaaatg taatagtata taaattcatt taagactaaa aagaataaga taaaataaaa
     6781 aggagtctgt atttctatgt attacttatt aaacaaaagc acaaatcctt actttaattt
     6841 ggcattagaa gaatatctat ttttaaatga taaatataat gatgatatta taatcatatg
     6901 gagaaatgaa gaatccattt ttataggtaa aaatcaaaat ccatatcagg aagtatatca
     6961 tgatgttatt gaaaagggag aaattcctat tttaagaaga atatctggag gaggaactgt
     7021 ttatcatgac ttaggtaata tcaatatgtc attcatacaa aaagataggc aacttcatga
     7081 gatagatttt ttggagcata ctaagtttat gcaaaagatg ctttcaacct taggactaga
     7141 tgtatctata actgaaagaa aagatttatt tttaaatggg aaaaaaattt caggctctgc
     7201 acagtctata aaaagaaaaa attctcttta tcatggaact cttctttacg attcagattt
     7261 aaataaatta actaaatatt taaattcaaa taaagcaact gaatcaaatg caactaaatc
     7321 tgtttcaagt aaagttacaa atattaagtt tttactcgaa aaagatataa attacttttt
     7381 agattattgt gttgaatatt taaaatctaa tataaagaat ataaaagaac ttgaattgga
     7441 tgaagaagat attaaaagtg tatacaatat aggagaagat aaatataaaa agatagagtg
     7501 gacatatggt aaaacgccga aatttcaagt agtgctacca tttaataatg aaagtaaaat
     7561 aaatatacat gttaacagat ggaaagtatc aaaatttttt atttcttatt atgataatgt
     7621 aattaatatg gacaagttgt tagatttgaa tttctttaaa gaagaaataa aagagagtat
     7681 tttagaaaat tatccacatt acatagattt aatcaattta atattttaat ataaaacctc
     7741 taatttgata aaattgtctt atatcaaact agaggttttt aatattcttg catattaaca
     7801 accattacaa aattgtaaaa ttaaaaacaa cactattgaa aaatcaatat atattgtaat
     7861 aaatatatgt gtttttcatt agcaaataga cattaactat ttatacaact gaaaatttaa
     7921 tatataatta atcagatggt taaaataaaa atattaaaag ttttaataaa tgatggttaa
     7981 aaataaaata cgaggtgata ccatgtatag tgttataatt gttgcagcgg gaagcggaag
     8041 gagaatgaac ttagatataa acaaacagtt cataaaatta agagaaaaag aaattattgc
     8101 tcatacaatt caagtttttt atgaaaatat aaatatagat gaaatagtgg tatgtataaa
     8161 aaaagaagaa gaagattttt ttaaagaaaa tataataaat aagtataact ttaaaaatat
     8221 aaaaatagca tatggcggaa aagaaagaca ggactcaata tataatggtt taaaaaaatt
     8281 agataaaaat tgtgatatag tacttatcca tgatggagca agaccatttg tcgaccatag
     8341 aataatcaat gaatctataa aggtagctaa agaaaaaaag gctgtggttg taggtgttcc
     8401 agtaagtgat actataaaga tagtgtcaga tggaacagta caagaaactc cagaaagaaa
     8461 tttgctgtgg gcagcacaaa cccctcaaac ctttgaatat aatctcataa tagatgctta
     8521 tgaacaagca tataagaaca attattatgg tactgatgat tcaatgttgg tagaaaatat
     8581 aggtcaaagt gtcactatgg taatgggttc ttatgaaaac ataaaaataa ctagtccaga
     8641 agatttaaat atagcagaac aaatattaaa tatggaaaaa agagatgaag ttaacagtag
     8701 aaggagaatt atatgagaat aggattagga tatgatgtac acaaattaac agaggataga
     8761 aaacttataa tcggtggtgt agagatacca cacgataaag gtttgttggg tcattctgat
     8821 gctgatgtat tgatacatgc aataatggat tctattttag gtgctctagc attaggagat
     8881 atagggaagc atttcccaga tacagatgaa gaatataagg gagcagatag tatgaaatta
     8941 ttagaacatg tgtataatct aataacaagt aaaggatata aaattggtaa tatagacagt
     9001 actataattg ctcaaagccc taagatggct ccatatattg agagtatgag aagtaatata
     9061 tcaaaagttt taaacacaga cattgataat ataaacataa aggcaacaac agaagaaggt
     9121 cttggtttta caggagcaaa acagggaata gcttcacaaa gtatttgttt attattgttg
     9181 acaagtcaga ataattaata tattattagt aataagttta atggatatca agtaataggc
     9241 ctatatagaa tatctataat gtatgaattt aagtgaaatt aatataaata taaaattagc
     9301 tatgataaag aaatagtaaa atgatgaaat cttcacagag agaaaacggt ggtgagagtt
     9361 ttcaagagga agtctttgaa cctgtctttt agcttaaagg tataaaaatc tttacggtag
     9421 gaaccgttat attcccaaaa gagagctatt catatagcta actagagtgg taccgcggaa
     9481 attaaacctt tcgtctctat acttgtatag agatgagagg ttttttatat tttcaggaag
     9541 ttaatattca ggaggaatct attaatgaaa atgtcaaaaa tgtttatgcc gacattaaaa
     9601 gaaatcccag cagatgctga gattacaagc catcaattaa tggttaggtc aggcatgata
     9661 aaaaaaatga cttcaggtgt ttataaccaa ttaccaatgg ggttaagagt gttcaaaaaa
     9721 attgaacaaa taattagaga agaattaaat aaaaaagatt gtcaagaaat actttgtgca
     9781 gccttacttc cttctgaatt atggaaagag tctggaaggt ggactgctat gggtgaagaa
     9841 atgtttagac taaaagacag aacagaaaga gagtactgtc ttgggccaac acatgaagaa
     9901 gcattcactg atataataag acaagaaatt acttcatata aacaattgcc actgaacttg
     9961 taccaaatac aagtaaaata tagagacgag agaagaccaa gatttggtgt tatgagaact
    10021 aagactttta caatgaagga tgcctatagt tttgatgtag atgataaagg tttagataag
    10081 tcatatcaag atatgtttga tgcatatgta agtatattta atagatgtgg attggagaat
    10141 agccctgtac aggcagattc aggagctata ggaggttcta catcagccga atttatggtt
    10201 aagtcagaag ttggtgaaga tgaagttgta ttttgtagtg gatgtgatta tgcagccaat
    10261 gtagaaagag cagaatcttg taatctagca tcacaaaaag aagaaatgaa agaattagaa
    10321 gaggttcata caccaggagc agctacaata aaagagttag aagaattttt aaaaacttct
    10381 ccagataagt ttgctaaaac tttagtatat gaggcagatg gaaaaacagt tgtagtcgtt
    10441 gtaagaggag atagagaagt taatgaaatc aaagtatcta atgccatagg ttctgttata
    10501 gaatttgctc ttgctacaga tgatgtagtg agaaaagtaa ctaatgcaga agtaggattt
    10561 gcaggtccaa taggtataaa tgctgactat gtatttatag ataaagaaat tgttgaacaa
    10621 agaaacatag ttgttggggc taataaaact gagtaccata taaaaaatgc taactatgga
    10681 agagattttg aaggaatagt tggagacttt agaaatgttc aagaaggcga caagtgtata
    10741 gtatgtggta agccacttga aatagctaga ggtgttgaag taggacatat atttaaacta
    10801 ggaactaagt actcagaatc tatgaatgca aacttcatag acaaagatgg aaaatcaaag
    10861 cctatagtaa tgggatgtta tggaataggt gttgaaagaa cagcagcagc tataattgaa
    10921 caacataatg atgagaaagg tataatatgg cctttgtcag tagctccata ccatgttgtg
    10981 atagttccag caaatatgaa aaatgaagaa caaatatcaa tagctgaaaa tatatataat
    11041 gatttgcaag ctatgggtgt agaagtttta ttagatgaca gagatgaaag aataggtgtc
    11101 aaattcaatg attcagaatt aataggaata cctatgagga ttacagtagg taaaaatatt
    11161 aatgaaggta aagtagaatt taaacttaga cataaagaag ataaagagat aattgacata
    11221 gaagaaataa atgaaaaagt aaaggcagag tttatcagga ataatgttag gttaggataa
    11281 taacttaaat aagatattgt atagataatc aaagttgtac tttatagaca ccattttatc
    11341 tatatataag gatataaaat ggtgtttttt tataaaacaa ataaatgagg aggtaattaa
    11401 aatggcaaaa aatgaaaaac aatttgttga agaaataaca aagatggaag atgattttcc
    11461 acaatggtac acagatgtaa taactaagac tgacttagtt gattatgccc cagtaaaagg
    11521 ttttatggtt gtaaagccat atggatatgc attatgggaa aagatgcaag aatttatgga
    11581 taaaaaattt aaagaaactg gacataaaaa ctgttatttt ccattattaa taccagagag
    11641 tttattaaac aaagaagcag aacacgttga aggttttgct ccagaagttg catgggtaac
    11701 acatggagga aataaaaaat tagaagaaag attatgtgta agacctactt cagagactat
    11761 aatatgtaca atgtatgcga aatggttaaa atcatataga gaattaccat atctttataa
    11821 tcaatggtgc tcagttgtta gatgggaaaa atcaactaga cctttcttaa gaacatctga
    11881 gtttttatgg caagaaggtc atacgttaca tgaaacagca gaagaagcac aagaagaaac
    11941 aatacaacaa ttagaagtat ataaagcgct atgtgaagaa ttacttgcaa tgccagttgt
    12001 agctggtcaa aaaagtgaaa gtgagaaatt tgcaggtggt gaaagaacat atactataga
    12061 agctatgatg catgatggta aagcattaca atcaggtaca agtcacttcc taggtcaaca
    12121 ctttactaag gcttttgata ttacttttgc agatagggaa ggaaatttag ctaatccata
    12181 ccatacatct tggggagcat ctacaagact tataggtgga cttataatga ctcatagtga
    12241 taacagaggt ttagtattac caccaagagt agctccaatt caagttgtta tagttcctat
    12301 agctgctaaa aaaggaaatg taatggagac tgttgataaa atatatgctg atttaaaagc
    12361 taagggagta gctgtagaag ttgatgatag agataactat acaactggat ggaaatttaa
    12421 tgagtgggaa atgaaaggtg ttccagtaag agttgaaatt ggaccaaaag atatagaaaa
    12481 taatcaagct atggtattta gaagagatac tttagaaaag gattctatgc ctttagaagg
    12541 attagctgat gctatatgtg atttatttga tgttatacac aatgatatgt ttgaaaaagc
    12601 aagaaaacat agagaagaca atacatctat agttgaaaat atggatgagt ttagaaaagc
    12661 tttagaagaa aagccaggat tcataaagac tatgtggtgt ggagatgcag aatgtgaagc
    12721 taagattaag gaagaaacag gagcaactat aagatgctta ccttttgaac aagaaaactt
    12781 aggtcacaag tgtgtgtact gtggaaaaga agcagatagt atggtagtaa tggcaaaagc
    12841 ttactaaaat acatttaaat aaaaactggg gtagaaagta tttaatactt tatcccagtt
    12901 ttttgttttt tatgagtaat aaaatgtatt tgtaactatg tcataagata agtcatagat
    12961 acttatatta aaaagaatat aatagagaac tatttttaat aattacatat taatgaaagc
    13021 aaaatatttt gttatataga ttttcaataa agcaaacaat aaatataaat gaatattaat
    13081 aaggagtgct tttaattttt agaaaataaa tgacttaaaa gaaagattta tgaaattaaa
    13141 actaggattt tctgatataa taaacatgtg tatttttaaa taaaaaggat attattatta
    13201 gatttgatta aaaataaact tgcaaaacaa tgtactagtt taatattata gttatattga
    13261 ataaaatatc aggaggaagt aaaaatgaat gtaagagtaa ggtttgcacc aagtccaaca
    13321 ggatttgtac atataggtag tttgagaact gctttatata actatttatt tgcaaaaaaa
    13381 atgggcggag aatatatatt aagagtggaa gatacagacc aaagtagatt agtagaaggt
    13441 gctatagaga atatgcttaa tgctatgaaa tgggctgggg tcaaccatag tgaaggtgtt
    13501 atattagatg atagtggaaa agtagtacaa aaaggagaat atggtccata tattcaatct
    13561 caaagattag atatatacca agaatatata aagcaacttt tagatagtgg aaaagcttat
    13621 tactgtttct gtacaaaaga aagactggat gaagtaagag atgctcaaag agcagcagga
    13681 gaaacagcta aatatgatgg tcattgtaag aatctttcta aagaagaagt agaagcgaat
    13741 ataaaagcag gaataccata tgttataaga ttaagattgc cagagaatca tacaattaag
    13801 tttacagact tagttagagg agatatggaa tttaatacta atgacttaga tgaccaagta
    13861 ttaatgaaaa cagatggatt tccaacttat cattttgcag tagttgttga tgactatttg
    13921 atgaaaatta cacatgttat aagaggagaa gaatgggtat catctactcc aaaacatgtt
    13981 tatttatatg aagcttttgg atgggaagca cctgtatttg tgcatttacc aaatatactt
    14041 aataaagaaa agaaaaaatt aagtaaaaga caaggtgatg tagctgttga agactttaag
    14101 aaaaaaggat atttaccaga gggattagta aactatgtag cattagttgg ttggtctcct
    14161 gaagataatc aagaattgtt tacaatggag gaattagaaa aagccttctc tgtagaaaga
    14221 gtatctaaaa gtggtggagt atttgatact gaaaaattaa attgggttaa tcagcactat
    14281 ataaaagatg gtgatgatgc ttatctaaca gacttagcaa taccattttt aatagaagat
    14341 ggatttataa cagaagaaga agctactaat aaatatgatt tcttaaagag tatgatatca
    14401 gtacttaaag agaaattaca atatgtaaaa gaagttactg aacatgcaag tatatttttt
    14461 ggagataaaa tagaggtaga aactgaagag ggaagagagt tcttaagatt agaacatata
    14521 cctactttaa tagatgcttt gagagaaaaa atagaaaaaa cagaggtatt aaatgctgag
    14581 tttgttcaag ctatgcttaa agaaatacaa aaagagtaca aaataaaagg gaaaaattta
    14641 tttatgggct caagaataat tttgacaggg caaatgcatg gtccagattt accaaaagta
    14701 atggaagttt taggaaaaga aacttgctta aatagaatag cttatgtaaa aaataatata
    14761 ttatagtcat aaaagtccgc taatttagaa gcggactttt tttgtgttag tatttggtga
    14821 aaatatctgc ttaagaaaat gaaggtattt aaggtaataa atttaatata taaaaagcaa
    14881 agcaaaaata taaataaaat atgaataata aactaataaa atattaagca tagaagaagg
    14941 gaatctatta ttttagtttg atttaattat taagggggta attactatgt atagtaatat
    15001 tgatgatgtt aaaaaggaat taaaggagct atgcttagaa tatgttacaa ttttagaaaa
    15061 attaaaagat gaaaaaatga ttacagaaga aacttttgaa aagtgctctt ctcaaaaaaa
    15121 aatattttta gaagaacaat agaggatggt aatagtatta ttattatgta gtttgaaaga
    15181 tagtacgaaa aatgatagta atactattat ttattgcaat tttcaactat aatataatat
    15241 ataatgttaa gaagataatt tataaaaata cagtgaggtg atgagtgtga aagtttataa
    15301 tacattgact agaacaaaag aagaatttgt tccattagaa gaaggaaagg taaaaatgta
    15361 tgtgtgtggt ccaacagttt ataactatat acacataggt aatgcaagac catttataat
    15421 atttgatact ctaagaaggt atttagagta tagaggatat gatgtaactt atgtacaaaa
    15481 ctttacagat gttgatgata agattataaa tagaagtcat gaggagggaa tatctcctga
    15541 agaagttgcg gctaagtata taaaagaata ttttgtagac tgtgatgggc taggaataaa
    15601 aagagctact gttcatcctc aggttacaga taatatacaa cagattatag agtttataaa
    15661 agagttggaa gataagggat atgcatatgc agtaaatggt gatgtatatt ttgatacaaa
    15721 taagtttgaa ggttatggaa aactttcagg tcaaaaacaa gaagatttag aggcaggggc
    15781 tagaattgag gttaatgacc aaaaaagaca tcctatggac tttgttcttt ggaaggcaaa
    15841 aaaagaaggt gagccaggat gggatagccc ttggggagaa ggtagaccag gatggcatat
    15901 agaatgttct gttatgtcta agagatattt aggtgaaaca attgatatac atgcaggtgg
    15961 gcaagattta acattcccac atcatgaaaa tgaaatagct caaagtgaag ctagaagtgg
    16021 aaaaacattc tcaaaatact ggatgcataa tggatatata aatataaatg atgaaaagat
    16081 gagtaaatca aaaggtaatt tctttacagt aagagatata tcaaaattat atgatttaga
    16141 gatagtaaga tttttcatgt tatcagcaca ttatagaaat ccagtaaact ttagtgatga
    16201 gatgttaaat caagctaaag caggtcttga aagactatat aatactaaag aaaaattaga
    16261 atttacgcta agtaatttag tagaatctcc tttaacagaa aaagaagtag agcttgttaa
    16321 agaattagat gactttagac aaaaattcat agatgctatg gatgatgatg ttaatacagc
    16381 agacgcagta agtgtaatat ttgaattagc taagttaata aattctaatg tagatgaaaa
    16441 ttcttctttg gagtttgcta aaaaatgctt agatgaattt aatgaactta caggagtatt
    16501 aaatattgta aataagaaaa aggatactgt attagataaa gacattgaag aattgataca
    16561 aaaaagaact gatgcaaaga aaaataaaga gtttcaatta gctgatgata ttagacaaca
    16621 attactagat atgggaattg tattagaaga cacaagacaa ggtgttaagt ggaaaaggat
    16681 ataaaaatgg aaaaaactga attagttact atgtcaccat tagttttagc atacttaggt
    16741 gatacagtgt atgagacata tataagagaa tatttaatta gacagaatac acaaagaaaa
    16801 gttaatgatt tgcataaact agctataaag tatgtaaaag ctaaagctca ggcaactata
    16861 attcatgaag ttgaaattga gttaactgaa gaagagagta aaatttataa aaggggaaga
    16921 aatcaaaagt caaatacttc tcctaagaat gcagatatta tagactacaa acatgcaact
    16981 gggtttgagg cattagttgg atatttgtat ttaaataatg aaatagagag acttcaatat
    17041 attatcaata aaggaatcaa aattattgaa agagatatgt aaatattaaa gagtgttgtc
    17101 ttaataataa gtaataaggc aacacttttt ttataactaa tgtataataa atatatagaa
    17161 attagtaatg agaaaggata aaaagttatg aaagtaaaat taatatcaca tacacctgaa
    17221 ccagaaaaag taatagctat ggcagctaag ctatgttatt caccagttgg gacagacgaa
    17281 atagaaaaag acttaacaga tgaaagtata gagaaatttt taaatatgtt attaagtata
    17341 ggtcatgggt caatattaga acatgcttca tttacatttt caatagaagg tatttcaagg
    17401 gcatgttcac atcaaattgt tagacatcgt atagctagtt tttcacaaca aagtcaaaga
    17461 tatgttaaat tagaacaatt tgagtacata attcctccag aaatagaaaa aatagaaaag
    17521 gcaaaagagt tatttataga ctccatgaaa aaagaccaag agaattatga taagttagtt
    17581 gaaatattgt ttgaaaatca ttataatgac ctaataaaaa acggtaaaaa tgagaaaaca
    17641 gctaaaagac aagctgagaa gaaagcaata gaagatgcta gatacgtatt tccaaatgca
    17701 tgtgaaacaa agatggtatt tactataaat gctagaagtt tgtttaattt ctttgaacat
    17761 agatgctgtg aaagagcaca gtgggaaata agaaatttag ctgtggagat gttaagagag
    17821 gttaaaaagg ttgctcctat attatttaaa aagactggtc caagttgtgt aaatggaagt
    17881 tgtccagaag gaactatgac ttgtggagat atagttcaag ttagagaaaa atttaaggcc
    17941 ttgtaggagg tgaataattt ggcgagtata gaaggaagaa atccggtaat agaagcaata
    18001 aaaagcgaca gagaaataga taaaatatta attgcaaatt cagctaaaga aggttcaatt
    18061 aagaaaataa taggaatggc taaagataag aatataatta ttcagtatgt tgataaacat
    18121 aagttagatg aagtaagtac aagtcattca catcaaggtg taatagctta tgcaagtgag
    18181 tataagtatt atgaattaga tgagctaata gatttagcaa agaataaaga tgaagaccca
    18241 tttttcatta tattagatga gataacagac ccacataatt tgggttcaat aataaggaca
    18301 gcagatgcag taggagcaca tggagttatc attccaaaaa gaaggtctgt acacataact
    18361 cctgtagttg ccaaagcatc tgctggagca gtagaatata tgccagtttg caaggtaacc
    18421 aatatagtta atacaataaa aagactcaaa gaagaagggc tgtggattgc tgcagctgat
    18481 atggatggag aaacttttta taaacaaaat cttactggtc ctcttggtgt agtaattgga
    18541 agtgaaggtt ttggaatatc tagattggtt aaacaaaact gtgactttat agtaaaaatg
    18601 ccaatgatag gtaatgttac atctttaaat gcatctgtag caggtggaat acttttatat
    18661 gagatattca gacaaagact ggataaaagt aaataggcat gaaaaaaaat ataaatcatt
    18721 acttaataat agatggctac aatattataa atgcgtggga caatttgaaa gaactggcta
    18781 aagaggactt agaagattct cgagaaaagc ttattgatga cattatagaa ttttctgagt
    18841 ttatgggtta taagactata atagtttttg atgcttataa tgttaaaaat tctcgagaaa
    18901 aagttgaaaa aagaaaacac ataacaatag tttatacaag agaacaccaa acagcagata
    18961 gttatataga gaaatttata acttctttat caaagtatga tgatgtaaaa gtagccacta
    19021 atgactatgc agaacagcaa atgattttag gaaaaggtgc tactagaatg tctgcaagag
    19081 aattaaagtt agagttagat cgttccaaga ataaaatgaa ggaaaaaaat atcggtttga
    19141 ggaaaaaaat tcagcgaaat tggttggagg aaaggttaga taaagaaaca ttgtcgaaac
    19201 ttgagaacat tcgtagaaag cgttgaaatt actaggttct ttagactata atatacctat
    19261 aaatttgttt tatatatggg ggagatttac atgttggtag caaaagaaaa aagttatgag
    19321 ttagtggaca attgtcagca ggatgagtat aacattgtat taagagcaag tgaaggggat
    19381 aaaatagcac tagagtacat tattactaaa tacagaaatt ttgtaaaggc aaaagcaaag
    19441 tcatattttt taattggagc agataaagaa gatataatac aagaaggaat gataggcctt
    19501 tataaggcag ttagagattt tgatggaagt aagaccaatt catttaaatg ttttgcggag
    19561 atatgtataa caagacaaat aataactgct ataaaaactg caacaagaca aaaacatata
    19621 cctttaaatt catatgtttc tttgaataaa ccaatatatg atgaagagtc agacagaaca
    19681 cttttagata ttatagctac aagtatagta acagatccag aagagcttat aattagtaaa
    19741 gaagagttaa aaaatataga atcaaaaatg aacgaattgt taagtgactt ggaattagaa
    19801 gtactagaat tatatttaaa tggaaagtca taccagttta tagcggacaa gcttaaaaga
    19861 gatgtaaagt caatagataa cgctttacaa agggttaaga gaaaattaga aaaacatctt
    19921 gaaaacagaa atgattaata gtaaaatact taccataagc ttattgtgag caaaattaat
    19981 ttatatcgaa agataaggga ggaaattcaa aatggctaaa gctaaatacg aaagaacaaa
    20041 acctcatgtt aatataggga caataggaca cgtagaccac ggtaaaacta cattaacagc
    20101 agcaataaca aaaacattat atgacagata tcaattagga gaagcagtag atttcgcaaa
    20161 catagataaa gctccagaag aaagagaaag aggaatcaca atatcaacag cacacgttga
    20221 gtatgaaaca ccaaatagac actatgcaca cgttgactgc ccaggacatg ctgactacgt
    20281 taagaacatg ataacaggag cagcacaaat ggacggagca atattagttt gttcagcaac
    20341 agatggacca atgccacaaa caagagagca tatactatta tcaagacaag ttggagtacc
    20401 atatatagta gtattcttaa acaaatgtga catggtagat gatgaagagt tattagagtt
    20461 agtagagatg gaagtaagag atttattaac agaatatgat ttcccaggag atgacactcc
    20521 aatagtaaga ggttcagcat taat
//
LOCUS       DAYASV010000067        20075 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYASV010000067 DAYASV010000000
VERSION     DAYASV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20075
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.067"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            150..605
                     /locus_tag="ACGI68_002295"
     CDS             150..605
                     /locus_tag="ACGI68_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138730.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(826..1086)
                     /locus_tag="ACGI68_002296"
     CDS             complement(826..1086)
                     /locus_tag="ACGI68_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138731.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTGIKGMLQNGV"
     gene            complement(1349..1702)
                     /locus_tag="ACGI68_002297"
     CDS             complement(1349..1702)
                     /locus_tag="ACGI68_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1375 family protein"
                     /protein_id="HFH0138732.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLNQSSVNEASNISSFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1719..7040)
                     /locus_tag="ACGI68_002298"
     CDS             complement(1719..7040)
                     /locus_tag="ACGI68_002298"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138733.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFIKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGNGNTMLIDNKGILKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDIFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNKSASQYT
                     EIGVSNVTDAYSSYNATFIVADEKKVYATGINTNYLLGVNPNDGVNINLTLINNWFYV
                     NPSGASYSTNSCTNDITKINNIIIYEYMAILCTNYGSFLTGYHGTLWTKPSDSTYRVQ
                     QQGITHAGYISNYIYNYYPTRCTQSSYSTNFAYLYYGESTSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHVFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNVFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYICNDVSIIVKNDNTCYVCGFVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNVIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAESNEPQA"
     gene            complement(7057..8040)
                     /locus_tag="ACGI68_002299"
     CDS             complement(7057..8040)
                     /locus_tag="ACGI68_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0138734.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKVD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(8041..8739)
                     /locus_tag="ACGI68_002300"
     CDS             complement(8041..8739)
                     /locus_tag="ACGI68_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFH0138735.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8736..9788)
                     /locus_tag="ACGI68_002301"
     CDS             complement(8736..9788)
                     /locus_tag="ACGI68_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFH0138736.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9781..10209)
                     /locus_tag="ACGI68_002302"
     CDS             complement(9781..10209)
                     /locus_tag="ACGI68_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HFH0138737.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDVLSANVKVSTIYGEVEINV"
     gene            complement(10209..10535)
                     /locus_tag="ACGI68_002303"
     CDS             complement(10209..10535)
                     /locus_tag="ACGI68_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HFH0138738.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(10551..12080)
                     /locus_tag="ACGI68_002304"
     CDS             complement(10551..12080)
                     /locus_tag="ACGI68_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0138739.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLNVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(12362..12784)
                     /locus_tag="ACGI68_002305"
     CDS             complement(12362..12784)
                     /locus_tag="ACGI68_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFH0138740.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12784..15237)
                     /locus_tag="ACGI68_002306"
     CDS             complement(12784..15237)
                     /locus_tag="ACGI68_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138741.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MILAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     VWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(15237..15404)
                     /locus_tag="ACGI68_002307"
     CDS             complement(15237..15404)
                     /locus_tag="ACGI68_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138742.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15431..15877)
                     /locus_tag="ACGI68_002308"
     CDS             complement(15431..15877)
                     /locus_tag="ACGI68_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0138743.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(16003..16431)
                     /locus_tag="ACGI68_002309"
     CDS             complement(16003..16431)
                     /locus_tag="ACGI68_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0138744.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYIGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16445..17509)
                     /locus_tag="ACGI68_002310"
     CDS             complement(16445..17509)
                     /locus_tag="ACGI68_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFH0138745.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESKVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17514..17957)
                     /locus_tag="ACGI68_002311"
     CDS             complement(17514..17957)
                     /locus_tag="ACGI68_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFH0138746.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEVSDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(18206..18646)
                     /locus_tag="ACGI68_002312"
     CDS             complement(18206..18646)
                     /locus_tag="ACGI68_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138747.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(18751..18948)
                     /locus_tag="ACGI68_002313"
     CDS             complement(18751..18948)
                     /locus_tag="ACGI68_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138748.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            19117..19437
                     /locus_tag="ACGI68_002314"
     CDS             19117..19437
                     /locus_tag="ACGI68_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138749.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19519..20025
                     /locus_tag="ACGI68_002315"
     CDS             19519..20025
                     /locus_tag="ACGI68_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFH0138750.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
BASE COUNT         6250 a         3543 c         2094 g         8188 t
ORIGIN      
        1 attttatgat aaaataatta gtatattttg ctttacttga atgtaattat ttatacaaaa
       61 gtcaaacata atataaatgt agtaaatttt tagtttcaag taatatctcg ttattataac
      121 taaatattta ttaaaattag gaggataaaa tgggcttaag agacaaattc gcgcaatcat
      181 ttgctagatc aaaaactatg agtggccctg agaaaaaggc taatgaaata atgggtaaat
      241 tgcttcttaa gaaagctata ttacctatcg ttttaatgtt cgtgataata atcgctggag
      301 ctatgcttaa aatcaattct tgggttacac ttggcataaa tttagttatt gctgttggtg
      361 ctttcttcta cataagaaat tctagtaaaa agtatcagaa ctttaaacct tatgttggca
      421 atttgattag tttagagaaa aaaggtaaaa aagagtacgt tgctattata aaacaaggta
      481 agttaccagt taagttacaa atagcttatg gtggtgaaga ccttgaacat gttaagaaaa
      541 atcaaatggt tcaaataagt tataatccag atgctaaaat agctattttg gttaatagac
      601 aataatttaa taaaaaaaag gagttgtctt aaaattgatt ttttctaatc aaatttaagc
      661 tcaactcctt ttttaatttt tatatattat ttttatatat tatttttatt acttttttta
      721 attaatattt ttaaatatca aatccttctc aataaaatag ggtactatct acaatactac
      781 tttatctttt cctaacaata tataatctgg tgctactctt tcatgttata caccgttttg
      841 taacatacct tttataccag taacatcttc cttaatttcc tctacatctg ttttcattat
      901 acccatttga acaagtatat ttttgtttat ttcttgttgt tgtgtagata attctataaa
      961 attttctaca gtttttttat acatgtctct atcttctttt ttctcctgca tagtattttt
     1021 aaataataga gcacatagta tacctattgc ccctagacta ctcaattcgg ttattaattt
     1081 atccattata gcctcctatt gtctaaattt tttatactta tagctttaat ttttgttata
     1141 ttcttacatc atgcaatatt atattttcag gattatcaat ttaatcttct gtactttaaa
     1201 ttgtaggtgc aatttataga ttgaataaat aataaatata tatacaattt ctaatataac
     1261 aaaaaataag tacaaggctt acttattaaa gttaagacct tgtactttac ataaaaaaaa
     1321 taactaatac atagttattc taaatttact attcaactgg tatttctaat tctttgcatc
     1381 taattctaac ttgctctcta attactgctg gtacttcttc tattgttttt ttgcctctta
     1441 aaattaaagc tacatatact tctaccattc cactctcacc tccagatttt cttacactaa
     1501 aagagcttat attacttgct tcattgacag aactttgatt taacaacatc tcatatattt
     1561 ctgcatttgc tataaaatta tttgttatat catcatcttg ctttacttga cctatactta
     1621 tgtctgaaat gtctttatac tgcatttcta cttttctacc ttgtttgatt tgttctattt
     1681 tatttttatc gaatttaaac aattttcaca cctccatttt aagcttgtgg ctcgttgctt
     1741 tcagcatcat atctaaaaat catagatgga cgttttatag ctgtccattt atctcctttt
     1801 tctactatga ttttaggctg agcacttttt atgttggctt cttctaattc atagatttct
     1861 tggtaacttg agtctgattt tttgatttca actagtttca tgtctttgaa taagtcagat
     1921 ttatttattt ttacttgaac tttagtttta tttattaatt gatatatagg attgttttcc
     1981 atcatgtcat cctttaatcc ctcatttaat gttactatta aatcagtgtt attttcagta
     2041 atgtttatga ttgaatatac tttattgttt atcattaaaa tagaatcctt ttttacttta
     2101 agccctgttt ctgatttaaa tataaacata cttgaatata aatcgcctgt aggactactg
     2161 gctttaatca gtatcttatt ttctcctaat actattttat tttgtggtat ctcaaatata
     2221 atgctattta tttccaaatt agtcttagta gatattaatt catcatttat aaacatttca
     2281 agtttttcta taacattatt agggtcttgt actacaactc tcacttgaga tattgatttt
     2341 tcaactattt ctatagatgc tttacttgta tctacttttg ttatcatgta tattgtatca
     2401 tctgaaagct ctatatctaa aatatcattt agatgttcag gaattggtat tttagtaaat
     2461 tcatataaat cagtaattga tatagatatt ttatacatag atttattttt accagttatc
     2521 catagactat tatcattttt aattactagt atgtggtcat cattatgtga cttaatttct
     2581 ttaacattag ttaatacttt agtaaaagtt cttattatct tatctttttc actttctcct
     2641 agtccaaata atccatttgt attgtaccct tgagcgtaca actctccctc attagttaaa
     2701 acaagtgtca ttgagttata tatacttata tctttaacag aaggtaatcc agtaaccttt
     2761 tgtaactctc ttacatcatt attgtggcca actcccaatt ggtactcagt attctttcca
     2821 tatgaccata gagtattatc tgtcttaata atataggagt agttatctcc acaagcaact
     2881 tttttgacat ctgatatatt tacttcagta agagttaata attctatatc ttcaagggtt
     2941 tgattaattt ctttatatga attacttcct attccatata acttgccatc cttagcaaca
     3001 gccaatgaat gtgtttttga tatatcaata tcagatacct caaatggcaa tattattgaa
     3061 gtatacttaa aattatcttt aaatatgttt ttataattta tatacttatt atttgttttt
     3121 atatacttat cattaaacat tactagcttt tcatttttct tattaaaaac tattttatct
     3181 gatgtgttat ctgttttaaa atatgaatgt agggaacctt caagagcttc atttttatca
     3241 ttatcatatc tatggtgaat acctaaatga ttatatctat tatctcctgt tgaagaataa
     3301 aattttccat atatatcttt aataaatata gtattgtaac tagaaaatac agtatcaaca
     3361 tctttcttta tagatacttt ataatatgga actgacatat ccctataaga ttcatcagat
     3421 ataccataag tatctctacc tgatgatatc aaattgttat tattatctat ataaagtgta
     3481 ttattagctg ttacaaaaac atctttaaca tcttccacac tatttgtatt attattattt
     3541 gctatacttc catatccact aagacctcca acaaatagtt tattgtcatt tgttaaaatg
     3601 gctgaagtat atccattaac tactattttt tttgcattta taatattatt aattactcgt
     3661 atacccttta tatcattagt ttctgttgaa aagaataccc tctctttttt ccctgtagta
     3721 taaataacat tgtcatttgt tataaaaaat gtagcttcac ttcctgctgt tacaacagat
     3781 tttacattct caatatttat ttttgtaaat gtacttatac ttccttcagt aaacccaaaa
     3841 tatccattta caaatccaca tacataacat gtattatcat ttttaacaat tattgataca
     3901 tcattacata tatacacatc tctaactcca tttataggta tacgtgtaaa tatattccta
     3961 cttgtagtat cacccaatcc taactgtcct tttccattat tacctgctcc atataaaaga
     4021 ccatcattag ttaataatag agatgtgttt ccatatacag ctacatattt tacattttca
     4081 acatttattt tagtaaatac atttctattt acattatctc ctattcctaa ctgaccagaa
     4141 ttatttaaac caacagcata acaggtatta tcatttttta tcgcaaatac atgtttacta
     4201 ttgtcagtaa atctatttac atttatagat tttatattat caatatttat tttagtaaat
     4261 acctttctat ttatctcatc tcccactcct aattgaccta catcatttaa accaacactg
     4321 taaagcatat tattattagt taataaatat gtagacttcg aatggctata tatttcttgt
     4381 atattagaag atatttcact actgctattc tttttattgt aatttatctt attatttaaa
     4441 tttaaactac tattaactga tataccatgc gtacccttag agttatatcc acatataaat
     4501 aagctcttat cagttaatat tgcaaatcta gttttatctc tacttaaatc ataaggaggt
     4561 ataaatatat tttttatatt tttcattacc tttttataat tagtggcatt atatccatct
     4621 tgtgtatctg aaacatctag ttcttttgca ctttcagata tattcccaaa accatataac
     4681 gtgttagaag ataaatacaa tagagcttta tataaaaata tggctttatc agaggtaata
     4741 ggacctgaat ttatattaga tgctgcaata ttattagatg attgattgtt aagataattc
     4801 cttccatatt gatgtatata agatgaacct ccagaaataa gtaaattatc tggatttaca
     4861 ttttttaaat tacttgttga ttctccataa tacaaatagg caaaatttgt agaatatgat
     4921 gattgtgtac accttgtggg ataatagtta tatatgtaat tactaatata tccagcatgt
     4981 gtaattcctt gttgttgaac tctataagta gaatcacttg gctttgtcca taaagtacca
     5041 tggtatccag ttaaaaaaga accataattt gtacataata tagccatata ttcatatata
     5101 ataatattat taattttagt aatatcattt gtacaagaat ttgtactata acttgctcct
     5161 gaaggattta cataaaacca gttatttatt aatgttaaat ttatattgac tccatcatta
     5221 ggattaacac ccaataaata gtttgtattt ataccagttg catatacctt tttttcgtca
     5281 gccacaataa atgttgcatt atatgaacta tatgcatctg ttacattgct aacaccaatc
     5341 tcagtatatt gacttgctga tttattacta agtccattaa cataacttga actctttcca
     5401 gaagtatata tttgcttatt attagttaca attaatgaaa accagtttga taaatcagta
     5461 tttataacaa ctttttttac attatctata gatattttct taaatatatc tgaattagaa
     5521 ggaagaacac ctgctattga gttactattt ggcccagaat aataacaagt gtcatcattt
     5581 ttaatgtaga atattcctcc agaaatattt tttgatgcta ctatagactt tacattagag
     5641 atacttaatt ttgtcaactc atttaattga cttggtaagt cagaacccat tcctttgtat
     5701 ctattatttc ctagaccata tacatctcca ttccctaaaa taaccagagt acaataagga
     5761 ctaaaaacga catctcttac agaatgtata tctagtggaa gttcaatatc ctttatttcc
     5821 atttcttctt ttaataaagt taatttatcc tcagatttaa ttaaatttat ataattacta
     5881 taactatatt tttcactatc cattttctta aattctacaa tgtgcatatc atcaaaaaac
     5941 gaacctaatt gtccataagt attatctcct gtacaatatg aatcattatt tgaatcaatt
     6001 agtatgctat gtgataaacc aatgaaaata ttcttagcta caatattttt taaatctata
     6061 aatgaattat ctatattctc agagtaattt gctataccta actgatatat atcattattt
     6121 ccagcaacct tcaaaatacc tttattatct ataagcatag tattaccatt accacatgca
     6181 atagttttta tatttacttc ttctatctta gtaaatagtg atgattgaag tgcatttcca
     6241 aaacattttt cctttccaca accatatata tcattattat acgtcattaa cacagtgtgt
     6301 tcagaaccac aagctatagt cttaacatca ttaatagaaa ctaagctaaa taaatctcta
     6361 ttattcttat ctcctaaacc tagttgacct tgattattaa aaccagtaga ataacattta
     6421 ttgtcattct ttattataaa agctgattta ttatatgaga atacagcctt tacattatca
     6481 acatctactt ttataaatgt atttacattt gcagatgctc cattagttcc tagacctaat
     6541 tgaccataca aattatttcc agcaacatat aaagttccat cattgtataa aagatatgtt
     6601 gattgaagtc cacatgcaat atcctttacg ccttttattt ctggaacctt ctcaaatgtg
     6661 gaaactgttt ttcctacttt ttctaatccc aatcccattt gaccatctgt atttatacct
     6721 gcgacaagta attctccatc tgaagtaagt ataactgcat gagattctcc acatgcaact
     6781 tttttaatat tagtagatat atttggtatt ttcgtaaata attttcttgt tgtattgtct
     6841 tctcccaatc caagttgaaa gttactatta tctccacatc catacaagat attattattt
     6901 tttagaccaa atgtatagtt acaacatcca gtaaaaaaat taataaagtc ataattatca
     6961 tacccttcat caaattcatc aactaccata tttttatcgc caaagaaatt acctctttga
     7021 agtagtttag ttcttttcat tataaaaacc tcctaattat cttatttcat acgaatattt
     7081 atttaaagtt atatctgtct ggccaattga atatgcccta ttagattttg tttcaacaaa
     7141 gttattatcc atttcatgta tatatctaac tctatttgca ttaaatggta gaaccacatt
     7201 cttaaatgta acactaccaa gttctccagt tatacacttt ctatttaaat ctaacttaag
     7261 gccttctata gctattatgt ttttagcatt agttaaggta tcataccaat atccagcttc
     7321 atctacatct atttctgact ctaattctaa gtatattaaa atatctaata ctttttcaga
     7381 taatttattg taactatttt ttaatacaga tagttcagtt tgtatttgac ctcctattac
     7441 atcatcaata gatatatttt taattttttc tactacctca tttatagctc ctgataagtt
     7501 tttagcttct gtagataaga gttcttctcc aatttttact tttatttctt ttatattttt
     7561 atcaacctta tcatctaaat cttgaatatc cttttgagtt acaaaaacca acgtcgggtc
     7621 tacttctaat gtaacatttg aagtattgga caattgtaaa atcattttta caaccaaatc
     7681 tttaatactg ccatcttcag cacgtggctt atacgtctct ggatacttac ctatagctat
     7741 tatattattc tcattatcca gaacagcagc ttctcttatc ataaatccac caactgttcc
     7801 tggtaaaata gtctctataa ctatccaatt aggatttttt tcatcaattc ttagagaatt
     7861 tatatttcct tcccaaacta cattctttaa agcagtttgt tcctctgttg gctcataata
     7921 ttctccatta ctatctccaa cttgaaactt ggttaatcca acaagctctc ctagtgcagt
     7981 tgcatttgct attttttctc taccaatttt ggttaatatt atataaaatt tttcttccaa
     8041 ttataccacc tccgatttag gataaactac tacattttct acgcctgtat catttgcaat
     8101 agctatatta atcttacaat tattttcaat gcatttaatg cttaaaggat aaacctttat
     8161 ttcttctcta cttaacattc cagctccaca gtttacaaca gcgctacatt ttataattgg
     8221 ttcaaagtat ggatgaactt ttacttcttc actacatacc atacctccac cacaatagac
     8281 catactttta tctagtatta ttggttctaa atagtgtaat aagtgagcag gttttactct
     8341 ttcaatcata tcgctacaat ctaaaagagt tttatagtca caattatttg ctataaaact
     8401 caatacgaat gtaaattcat cactataaac ttttatatct gtttctgatt ttgtatatgc
     8461 ctcacatata ctttttataa cttcaatagt actagtacct ctgcttctca ttttagcttt
     8521 tatattgcta cgtcttgtgt caaaatcaag ttcttttttt tcaatgcaga gtatgtcttc
     8581 ccacatatct aatccccatg ttgcagtgtc aacaaataat tggttaaagg tatcatcaat
     8641 atcttcttta agaatattta attctgtatc gtatgcacct tgtatctcct ctacaatgta
     8701 atttctatca aatgatggta gtttatcaat tagcttcatt gattttccac ctcactaaaa
     8761 ttaacagttg ttacactagg tattttatct tcattaatag ttatattatc tgtacttcca
     8821 tttataagta ggttacttaa atcgtgtaca ccagtagtat ttataagtat tcccattact
     8881 tttgtataga ttatctctcc tctaatatct ctaaagtatg tatttatact ttctaggaaa
     8941 gattctttta cattgtctaa tgtatatcca tcttctaatt tcattactgc acttatattt
     9001 atttctattg gtaatggtgt cacaactgtt atagttggtc caataggctt ctcttcatct
     9061 atatgttgct gacacctttc aattgtttct gtatcaacag cttgattatt tttcccaaat
     9121 atcaagacct taactgttcc cggaccatcc catcttggat aaaccttaac attatagact
     9181 ccatctactt ccaaagccca ttcttcatag tgagctttat ttccacttgt agcttgattt
     9241 ctttggattt tataaaatct ttcttttagt tcttcatctg tctctatatc tgtaccacct
     9301 tcaaaacttc ttgtgttagt aatctttgtt actccagata tattatcaac tagtttaaat
     9361 tcacaatttg taggtaaatt atatttctta ccaacttcca gagcttgaac tgggctattg
     9421 tcaccttctt cactaccaat agttacatct tttattacta caaatagtaa atctctatat
     9481 gatattattg tgccattatt tattacagtt cctttctctc caataaattc aacttctcca
     9541 tttgactctg tacctaactt tctatataca ccaaattcat tgactctttt atcgagaaac
     9601 tggttatatg tgtcttgtat aaaagccatt ttatgtatct ttgaaagttc tagatatatc
     9661 tttgaaagtt ctagattatt tccagatacc atgttgttta gaaaagaacc ttctccttta
     9721 taaatatcaa gattaatatt ttcaagagtt ctatttttta ttacttcata tgtctggtca
     9781 ctatacattt atttccacct ccccatagat agtggatact tttacatttg cactcaatac
     9841 gtctctgtta aagttagcac tttttacgtt tacttctaat atatatggat ttattagaag
     9901 ggcctctttt atgaatctac tagcttcact ttctgtaaga ccttttgtat atttttgtcc
     9961 tattagttct gaaagctctg tcccatattc taaagagtat atctcatgtt catatctatt
    10021 tgtctttata cacttgtata tccaaacttt taaggcttct tttttttcaa ttattttaaa
    10081 gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc catgccactt cacgaaagat
    10141 tggcaattct tctgttttag gtaagatata atcctctggg acacctataa aaggaaatat
    10201 tgtactcatt ataagctcac caacttactt acaacagcaa atttttcgcc tattctaaac
    10261 attattacca tatccccaga ttcaaaatta tctataaaag gattttttac ttcatgctgg
    10321 tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc tatcaatcat ccaactatct
    10381 atcaaaatat cttctttttc taatatgatg ttatttgtct ctatttttaa atctggtaat
    10441 ttacttttaa tttttccaat aaaaaaagaa ggttgattat gatactttcc ttcttctctt
    10501 attattccta taaattcatt tattggatta gccattatat caccatcctt ttataatact
    10561 ctcctagctg tattaaaatc tcttctacta cttaacttac ttattttaac tacatcacct
    10621 ttttgtggag catgtaaaaa ttctccatta cctatatata atccaacatg gctgactgga
    10681 ttatgaaaaa atactaaatc tcctgcttga agattttttt gttctacttt cttgcctttt
    10741 ttagattgtt gatttgacgt tcttggcaaa ctaacattaa cttttttaaa acaatagacc
    10801 attaaaccag aacagtcaaa actacttggt ccatttccac cccatttata aggtttacct
    10861 agatgttttt ttgcttctga tattactatt ttagctttat cagatacatt accagaataa
    10921 ccaccaataa taatttttcc ttttcttctt ccaaatttac tagcttcttc aacatttcca
    10981 aataaaatat ctatatggta tgttccattc ttttctatag ttattgcagg tcctacgtca
    11041 tttactttat agactccatc acgaccagaa actcctgtaa ttacctgtat ttcatctcca
    11101 tagtttaata aaggatgttt atttaaaaac tcttttgtat aataagtttg ttcatattta
    11161 ccaaccatag gagcagcgca agttttttta gatgggtcaa gttttttctt tctacaatct
    11221 gtatctccac cttcttcttt tctaggacag taagctgtaa attctgctgt aaactctttt
    11281 cctcctgcat aatcttctcc ccctaagtta ctttcttcct taggttcatc ctgtcctgct
    11341 gacttttcat ccataagatt ttgaaaatta agttcaagct caatttgata ttctccattt
    11401 tgccaagtat gtttgtctgt atctatataa aatagtccta caagctttgt ataagaatct
    11461 ttaactttta ctcctctacc agttatacaa cttacatctc cataaccttt aagagaacag
    11521 cttttttcta tcccattaaa ctcgctatca atatctacat cttgattttc ttgttgctga
    11581 attacttttt gcattattac atttacttcc ttaaaaattt cattatctat tttttcgcta
    11641 atcttgcttc catactggtc taccactatt actttatttt ttacattttc catgctctcc
    11701 gaaaaggtgg tatttataat attaaatccc tcttcaaaca taacatttaa tgtaacagtt
    11761 cctttttcaa taacattaaa cttatctaaa ttggcctcta tcatatactt tttctttgtc
    11821 tttttacttg cttctgtata tgcactcatt attgtgtcat aaccatttac tcctataaac
    11881 atctttgtat acttgacatt ggtctttgct attgttccaa ctgaaagcct attttcagca
    11941 aatacttgct ttgctatgtc ttcaactaac ttatctttaa aattgtatga cacttcactt
    12001 tgtgtaagta aaaatcccat atctttagat acaaaactaa tttcattact gcttgaatct
    12061 ttagacctat taattatcat tcctctaaag agttctttct catctacata gaaacagact
    12121 gtactagcta ttggtatatc tatttgttgg aaatttacat caaatgatga ttgtattatt
    12181 gaaaactcta gtgtccttga tggagattta taatcacctg accatgatac cttgtctact
    12241 atgtcagtta tgtcatatat acttccgttt tttatgtgta cccaaatctt aataataaaa
    12301 aatcacctgc cttttcattt aattctataa gcagttattt ataacttatt atctataata
    12361 gttattgttt tggagttact tttggtatct ttattctttt atactctttt aaatctaatg
    12421 taaaatatac atctcgagtc ccatctcttt cttcataact aaacccttca attatgactt
    12481 ccatatttat atttgtttcc gtaattgtaa atcttaatat aaaaccttcc ttcatccatt
    12541 tttctatctt atttacacaa tcatatggtg atggaaaacc tgtatagtca cagaaagtat
    12601 agtctttatt tggaaaaaaa cttgatattg aaatgctttt aagagctaca ccaccaaaag
    12661 ttgctatttc acctattttg agtatactag aagagtttac agcagctttt ccattaatac
    12721 taaaggaaga tggaaatact ggaaatctaa atctatcttc agcttgtcta agccacattt
    12781 ccattataag acacccccta actgtgccaa ttggatactt gcaactaatt ttgatgttat
    12841 cttttctata tcagcttctt ctctaatcac tattgtatca gctaacttag ctatgtttat
    12901 atttccacca ttttttctgc ttctatattg atttgcttct tgtttagtta aaaccatttc
    12961 accttcatgg agtctggttt gatagtcatt ataaggaaca tagttcaatc caaatgcatt
    13021 attacctttc ttacctgaat aagctttacc agaatcacca gcctttgttc cagcatatgg
    13081 attatctttg taattttttt tactcttttc cactagattt aaaactgttc ctgtgatgct
    13141 tccatttttt atcttattaa tcatagtttg ccaccacttg catatatctt taactatttt
    13201 acatacttta tctaatatgt taaaaaaagc tcccaatatt ggctctataa ttttccatcc
    13261 agtttccaac agaggtccta aggtctccca tacagttttc caaataacac ccagtgtctc
    13321 tataaaattg tttatttgag gtgcatattc acctaaaaaa gtaaaaattc ttgtgataat
    13381 aggactaaca gcttcaacta caggtccgat tagttggaat gctgtagtta ctattgtacc
    13441 aaatatactt attaatcctt gtgcaattgg tgatttattg tttatactat ctagtaaatt
    13501 atttactaag tttactactg gaagtaatga atctattaat gctgtaaaat cgatattttg
    13561 taatagtgta cttcctgcgt taacaattgc ttttggtgca ttccctatca ttgttgctgc
    13621 atgattttca aaacgttctt gcttattttc aaccatcatt ttacgctttt cagcctcatt
    13681 agaaattcga tattgagtag aatcagaaaa ctcttttcca ttttttacac ttctagattg
    13741 catctggtca tatttgttgc caataattat cttaccattc attacattgc ctattttacc
    13801 agccattttt tcagataagc cagctaaatt gcctaatcca ctttctataa aagtttttat
    13861 tttctttagt ccttgaccta aaatatcatc aaaaggttta acaatactct tcaagccatt
    13921 gttaatgctt gtttgagctc ctgatattat acctggtatt gtattactca tttctttagc
    13981 taaccccatc tgctttacac ttatgccttc caatgcttca tcaaaagttt tataattttc
    14041 tcctaccaga gtattaagag attccatgtt tccttttgct gcacttaaga aagctgctga
    14101 tacatcttgt tcactacttg tattcatatt taaagctcta acatctacca tagcttgagt
    14161 tatatctttt tcttttttgc caccagtaat attactaatc attttagctt tagttcccat
    14221 tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc attcttacta aactgccata
    14281 ataatcatca acatctattt gattaacttt tccagtattt cttttcatta cactttctag
    14341 aacaactttc tgattttgtt cctgaccaag taaatcaaca ccctttttca cagcgacacc
    14401 tatagtagca ccatttgtca aatcagatat cttccctttt atcgaaccaa gtattccact
    14461 tgccatatcc tttacagcta ttgcaccact ccatacctta cctgcaaata atctcaattt
    14521 gggaaatact ttacttataa tgccacttgc catatctttt atagctatta caccactcca
    14581 aaaagtattt ttaaacaaca ttaacttagg caaaattcta cccatagcac tactcaccat
    14641 atctttaaga ttaatacttc cttgataagg acgtttctct acttgtttta attttcccaa
    14701 gatactactt ataactctct tagtcatatc ttttgctttt attacgatat tccaaacctt
    14761 gccaccaatc ttttttaaat ttccaagtaa tttattttta gttttttcta cttcatcact
    14821 tttagcttta aatattaata taatcataat atttgaatta aactcatttt tcttttgaat
    14881 caacttatta aaataatcaa taatcatagc tatagttatt gctagtatca taatacttcc
    14941 atttaaactt ccaataactc ctgaaacagc atttatgcta gaacttaatt ttgtcatagt
    15001 tgaacttaca ctactagcag tagtttgaac ttttcttaac gaggaattta aactgtttac
    15061 tctattactt gcacttccaa tagaattttt tatgctacta gccattacat tagtggaaag
    15121 tgaaacagcc acagttgctc tcctaaaatt atcagttact gatataattt gattcatttt
    15181 tggtgtgtaa ttatctgtaa ggtacatatt tataccttct tctctcacat ttcccattac
    15241 ataccacctc ctgtcaaagg acacactcca tatccatgtt tcattttttc aacttcttcc
    15301 tcaatttcta aggcgaaaaa agcttcaata agctttaatt cgcctttatc cattgcataa
    15361 aaaagggacg gtcttatacc ttttttcttc caataatagt acattattgt agtcctggta
    15421 tccgtcccta ttagtttttt acctcttcta tcactgcatt tttaccataa cccataacat
    15481 cttgaagtat tctatataag ttggtaattt cacctggtaa taataatttt cttgctaatt
    15541 cttttggtgt tggagcctta aatttatcca ttagctcctt atttttaaat aaaagctttc
    15601 catccaaatc acaaacacct tctataattg taagcatctg catctccggt aagtctatat
    15661 ccgttttcat attttcatca attttaagac aagtattttg tatttcttca tctttactta
    15721 ttgttaaagc tctacacatt actttaaatt cctgcccaaa tatagttgat aatctcttaa
    15781 gttcaactat agtactcggt ctttctaagt tttctgtatc tgaatttaaa agtaaatcta
    15841 ctatgtttat attttttgat aatccatttt cactcattat tatttgctcc tttatcaatc
    15901 aaattcaatc aactaacttt tttacttatt aactatctga atttattaag tatatattta
    15961 ctaaatatca gtataaaacc aaatataaaa ctaaatataa aactaaatta tatcaagaaa
    16021 atcatactca gtaaatgtga aaggtgcttc tatctctcct tttacgccaa cctcccaatc
    16081 agctaaagtt aaatcatcaa atgctatatt ctttactgct attctttctg ctccataaga
    16141 atctgggtca tttaatttag atatagcaac aaacctaggt tcagaacctc tctttatctt
    16201 ttctccaatt aacttgtgca ttcttgaact aacgtggtat agagttattg agccttttcc
    16261 tttatatcct atatactttg tatcagtacc catttgacct gctattataa tatcttcttt
    16321 tgtaaattcc atctttgctt gaaatttctt tacttctgct actttgtttc catcaagcca
    16381 aagttctccc caagtaccac tcattacatt tctagcttcc atatttgcca tattaataat
    16441 cctcctatat ttctattgat aaatctatat cttccatagc atcaagtact tttatttttg
    16501 cttttaaaaa tactttagaa cctgtgttag cttcttttat ttcttgtaat gtcatataag
    16561 ataaatctac tccttttgat tttaaatacg atttttgtgc ttcaaaatct atttcaacag
    16621 tagaatcaga ttctataagt gctgattttt ctaattcttc taaataactt tttatagcta
    16681 ctatcaataa acatttgttg tcataactgt tagtaacctt tcctatatag tcatctatta
    16741 tcacctttct tatgtcacta tgtataatat ctaaagtgtc aactattttt attttctgga
    16801 acatttctcc tttttctgct gttaattcag ttaaagaatt tactcctcta gcaattctta
    16861 tagctcctgc ttctttaata agtataagct ctcctttatt aacctttgat tctgcatcaa
    16921 ctttcgtcat cttaggtata tcgactacat cactaagttt agtataagtt actgattgac
    16981 ttaaaggtgt acctgctata agtccagcca ccctacttgt aaactcatca acactgtatt
    17041 tcttttctcc aactaacaca tcttctgtag taaaattaat tatcccttca tgatttccta
    17101 caacttttcc taatacagct ttaaccttaa ccttatctat atctctaagt ttaattatcc
    17161 aattttttat agcagtctta tcagcttcta ctgcttttgg catacataga taattaaatt
    17221 ccttagtctc taaaaaatct aatgcagttt gaatatctgc ttctccttct attacataaa
    17281 ctaataactt atttggagtg ttaacatttc ccatcaaagc taaatttata tattctttat
    17341 tttcagcaga taaatcaact ggtatatcct ctttttcatg tatttcatta agacctagtg
    17401 ccttagcatc tttaagtacc attgcaatta ttcctctagc tgaacgttct ttaacagttg
    17461 tagctagctc cttaaatgat atgttgatac ttggtaatcc tatagccata ctatcactct
    17521 cctaaattta aattgatttc ttccatattt tcatatacta catcttcttc atatacacta
    17581 tccagatagt ttagtgtaaa tgtaaagttc aatacataaa ttgactcttc ttttttaata
    17641 ctttgttcta gctttgttat atttaatttt ctgtctgtta cctttatatt tctgttgaat
    17701 aacttattta attcatctga aacttcatac attctaatac tctttgactg tggttttggt
    17761 aagtatttta tagagataac aatatctttg ttattcgtat ttaacattac aggtgtacaa
    17821 ataactgata gtatacttac aaaaaaacaa gacccttcag agccttgcac actttcatca
    17881 tcaataaaaa tactttcagt aaagtttttt ttgagaatct caataattgt ttctaatatt
    17941 tccttgtatt taagcaaata atcaccacac tatcactaaa tattcttgct taatctgata
    18001 atcttactat aaatgatata agtcattttt aaatatatat ctcatttatt cctgtcaagt
    18061 atcttctata tttttaggta atattattct tttcaatcta tcacctcctt caatatggat
    18121 ttctttcaca ataacattat ctcacatatt ttattgccta ttgttgtaat ggtgtatata
    18181 aagtgttgta aaattgtcct attttttata atatctcact caatttaaat atcatctttt
    18241 tctttaaatt gtagcaagat gttctatcta agtgtagttt catagaaata tatgtcatat
    18301 tgttttttgt cttactatta taaaaaagat taaaaaattc catttctata tcatttagac
    18361 acgttagagc attttctatc ttctcttttt ctatttctat atcttctttt tccattttta
    18421 attttcttaa tctttcctct ctttttaaca cctcattttc cacagaagag cttatgttat
    18481 atgtgttact agtcttttct gtatatacca ttgctccaca tccagagtat tcattctcac
    18541 atctttttat gtccaagtta atattcttta tctttatatc caaatactta taattatgta
    18601 atcttttatc tgtttcttta aataatatat cttttttgtt ttccatagta cttccccttt
    18661 tttatatact tattattagc ttcaaattta caataatctc tttagtgcac agttttttac
    18721 taatctcaca accttcaaga acatattgtt ctataactgt ataaaaaaaa cttcttctat
    18781 attttgttct aaaatactag aaatcttctt agccataaga agtccaggat tttttatgcc
    18841 tctttctatt tgagatatat aatcatttgt aattccagcc tttctggcaa gttgaatttg
    18901 tgtcatacct ttgcattttc tataatattt caagttattt ttaaacattg ttttatacct
    18961 ccattactat taagaatatt ttgttcttcc tcacttagat tatatagaat tatttgttct
    19021 ttatcaatag attttagaaa aaaatattct tttatatttt attagtttta ttttttagaa
    19081 caatttgtta taatctatac ataaggtggt gttataatgt ttgccaaaag attaagagaa
    19141 cttagaaagg aatttggatt gactcaaaga gaacttggag aaaaagtagg cgtttcccaa
    19201 agagtcttag ggtactatga aacagaaaac agatttcctg atgaacacat tttaaataaa
    19261 ttggctgatg tatttaatgt atcagttgat taccttcttg gaagaacctt agttaaggaa
    19321 aatattgata cagtagctgc acatagaaaa aatccacatg aagagttacc tgaagaagca
    19381 caagaacaac ttaatgatta tatagaattt ttactaaata aatataaaaa aaaataaacc
    19441 atatgatgag cagtctattt ttctgctctt ttaatataat tcaaaggcaa acatacattc
    19501 tattttaggg ggatttttat gaataatttg gataagcttt ttgaattagc ttctcaagaa
    19561 gagataataa ttcattatac cacttatatt gcaggtgatt tagaaggact atatataaac
    19621 aaacatggta ttaaaattat atcattactc agtaatttaa aacaaaactc aaaaaaattg
    19681 acatccatct tagctgaaga acttggacat catttcacta gccttggata ctatgtatcc
    19741 tcttataacg attattatac aaaaattatt atagataagt gcgaaaataa agccttaaaa
    19801 tgggcttgtg agttcttaat tactgaagaa gatataataa acataattaa ctctggcatc
    19861 acttgcgttt atgaaatggc agacatactt aatgtggata ttactttctt tcaaaaaaga
    19921 ttagaatttc tatcattaaa aaaacagtct ttacaacttg gaaataataa atatttaata
    19981 ttaactaatt tgccgtattt ctacatattt gatccaattt cataataaaa atagtagtaa
    20041 ttttaactgg cttaacagta aaatctatat attta
//
LOCUS       DAYASV010000068        18947 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYASV010000068 DAYASV010000000
VERSION     DAYASV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.068"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(208..1785)
                     /locus_tag="ACGI68_002316"
     CDS             complement(208..1785)
                     /locus_tag="ACGI68_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HFH0138751.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
     gene            complement(2082..4493)
                     /locus_tag="ACGI68_002317"
     CDS             complement(2082..4493)
                     /locus_tag="ACGI68_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0138752.1"
                     /translation="MRKYKLKKLSKLLVLLTVCFLIVSTIPVSAEKNKILTGVETAEY
                     SESYLQYLEDVKNGDIAKYNGVIPTPYEMEGTTLKTNVRSSLPASYKSSVSYDPRKLD
                     LTTPAKDQGKLNTCWAFSGMSTLEAYLKLKGYGTYDLSEEHFRWWSTGGIHGWNLTDM
                     TGSSNVTAIGYLTAWAGPKLEKDIPYNFKSEDEGATRPQNMDTAPTQFNVTDVVRISK
                     DKTSVKNAIMQYGAVTSGYAHYSAYLSDDENSYNCNDKSEPLNHSVSIVGWDDNYSKD
                     KFKPSVRPESNGAWLVKSSWGEFNSEKGFFWISYEDKTLLKDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETD
                     IDGYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            4668..5084
                     /locus_tag="ACGI68_002318"
     CDS             4668..5084
                     /locus_tag="ACGI68_002318"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFH0138753.1"
                     /translation="MEENFLILKKHLETILYLFFGALTVLINIITYLFFTRIINFTFL
                     LSNSLSWSIAVLFSYITNKFFVFKSEKTEFLFLLKEFVSFLSCRLFSGFIEILLMYLM
                     IDLFSLNDILVKIGTNIIVIILNFVFSKVVVFKHRN"
     gene            complement(5149..7266)
                     /locus_tag="ACGI68_002319"
     CDS             complement(5149..7266)
                     /locus_tag="ACGI68_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0138754.1"
                     /translation="MQISKKIISLLTAAFLSIVLPSNVVNASNKDSLIGSGRWETAIK
                     ISQNGWLRSDNAVLVNDNSIADALSATPFAKLKDAPILLSGSKELDFRTKSELKRLGV
                     KNVFLVGGSNALGVNVENELKSEKITVERIFGNDRYETSLKLAEKLNNEKDISDIIVV
                     NGEKGLADAVSVGSIAAQNYMPIILSDSNLGVEVANKFIKAQNIKNSYIIGGLNSISN
                     EIEKNLPNSKRISGNNRNETNANVIKEFYKETDLRNAYITKDGMKKQSDLIDSLSVGV
                     LAAKNNSPVVLVGNKIDSIQKDVLNTKIFSTVTQVGGIGNENVYKDILDMQEETKYTV
                     ETIEELDSALKKVDANDIIKFRPDKKISESFKLETEKAIKVDLYGEYSQSITINMPNG
                     ELNNTGDISKTLILKDIKDGTLTNSGYINQMDISDDNGCKIENENNGQIWIVTIFSSA
                     KDVNIRNDGTITKIDNDSSSTNIRNSGKIDTVSGNKEPGISGNKPNTNNTDKDDDSDT
                     AAGLSPKISACSPLENNQIMLTVPQKPKNSNYKIYYRVLSYKPSAMRVGDRININDWD
                     LVEDTEPLKVNAKNGSYIEVVEIDYTRKKVSRWGRAGATDDGFKIVANGIKSTVSVDE
                     NSKVTISVTGYNGNKVYYRIKTTKPEAMNVGDEIDSSWKSTSNSDIILEGNKVNGLYV
                     ELASGENNKVEKWGATDKIEIVS"
     gene            complement(7297..8004)
                     /locus_tag="ACGI68_002320"
     CDS             complement(7297..8004)
                     /locus_tag="ACGI68_002320"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HFH0138755.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELSKEKAKEIILKYLEEYPEAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKKWNPESGHYAIGYHSVK
                     SHFDDLELNKTQYVHAP"
     gene            complement(8111..9982)
                     /locus_tag="ACGI68_002321"
     CDS             complement(8111..9982)
                     /locus_tag="ACGI68_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HFH0138756.1"
                     /translation="MNKKNLSVIMAAAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRGLMSQKYTGGSQVGQPIYEIKVGETLSKLKIITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEATEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTETVTSKTIYVGIGDEKVDLTANPVDSTGTNLDPSAEGFRVNKIDK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVANGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKATELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGDDRHMTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSADAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKNALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI68_002322"
     CDS             complement(10326..12671)
                     /gene="secA"
                     /locus_tag="ACGI68_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFH0138757.1"
                     /translation="MSVLDTILDKADEQEIRRLNVIVDKIESLEKDIENMSDDELKKM
                     TDLFRDRLKKGETLDSLLPEAFAVAREASKRVLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVVTVNDYLAQRDKELMQPVYEFLGMSVGVIIADLI
                     PEVRKVQYNCDITYGTNNEFGFDYLKDNMVTKKFDKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGEGDEDSKLYELANSFIKTIKEEDYELDRKDKTVALKESGIDKAESFFGIKN
                     LTDIENIELYHHINQALRGHKLMEKDVDYVVVNQEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEIKDESKTMATITFQNFFRLYNKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPIIRADLHDKVFRTEKEKYQAVVEEVIRIHKTKQPILIGTVSVEKSEMLSGMLKK
                     EGIKHQVLNAKENDKEALIVSKAGKLGSVTIATNMAGRGTDIALGAGDKEEESRVKEL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKSIEKLMKR
                     PNSNKNNSIESKSLTKAIERAQKGVEGKNFELRKNVLKYDDTINEQRKVIYNERNKVL
                     NDEDIQVDIQNMVKDIIKEAAAEYLTKRKRDYYGYFKHLYNTFMPADTLLIPDLDNKS
                     IEDIIDSTYEISKRVYDLKKMVVGLDKITEMEKIVLLKVVDQYWVEHIDAMEQLKQYV
                     GFKSYAQKDPFKEYAMEGYEMFESLNKNIRETTIQYLYKFN"
     gene            complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI68_002323"
     CDS             complement(12907..15087)
                     /gene="slpA"
                     /locus_tag="ACGI68_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HFH0138758.1"
                     /translation="MNKKNLAVVMSAVTVIGSAAPVFAAAEDSFPNGNTTVSSSKYSD
                     IERILKKYVADGVTGITVNFFDKKGNPKGVSISSLEASQISYVKDEIKDLKAGEYAKI
                     SVKITDGTEKKYDDTLLKELDSNAVVTKVGTATTVKIVDQDGNSIENDAKLKLQKYDT
                     TTSAYVDGATKDEVLAATKAAVKTSKFTDKTGNNATGTVAITATYADAVAGAIKLKYD
                     TQAAVAPTLDTAVASLDGTKEIYDFSKPVITVNTTDGTSKLSFEKTGEKVGAVESNVT
                     VIAASDETVTISGDAKEKAEALAKKYVFKDTELEDAYKTVTASDFEKTDNDYYEVVLY
                     PTGKRLNTASTYASSNYKPELPTSDRVDTPAIITLRSTNKNNLKSALDELRTYNNSYS
                     NNSVLAGDDRIETAIEISKDSYNADGGIKGDYVEANEVVLVGSQSIVDGLVASPLAAV
                     KDAPLLLTSKDKLDSSVKSEIKRVMGLDDKTGITSKKTVYIAGGENSVSKEVANELKD
                     MGLKVERLSGDDRYATSLEIADEIGLNHNKVFVVGGTGLADAMSIASVASNKEMPIVV
                     VDGKGKDLSTDAKDFIGSAYVDIIGGKSSVSEDMEDAIDDATGKSPERVSGDDRQDTN
                     AEVIKTYFEKDNSDSVISTGVKNFYVAKDGSTKEDQLVDALAIAAVAGHNEAPIVLAT
                     DSLSSDQSVAISKVTNSDDSKKLTQVGKGIADSVIKRIKDLLEL"
     gene            complement(15380..16978)
                     /locus_tag="ACGI68_002324"
     CDS             complement(15380..16978)
                     /locus_tag="ACGI68_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0138759.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTVILVNTDNSIVDGLSASGLSGVVKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVADQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNNLKPNIEGTVDTSKAGTYVLNVSAFDNFGQKCEINVNVKVIINTSTKDPNS
                     YEFKAMVSNEMYNLVNSYRKEKGKKSLRELDSLAGMANAWSKYMDEKKIFAHEIDGKN
                     AAEVFSGFGMRTDENIAYLPMNVKSVYTSKDAKEIAKSVFDLWKKSSKYNDNMLKEEF
                     YSFGFGMYVSNQREVNATMEFLNS"
     gene            complement(17197..18798)
                     /locus_tag="ACGI68_002325"
     CDS             complement(17197..18798)
                     /locus_tag="ACGI68_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HFH0138760.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVIQQCISASKSNGQPPAITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLVVSSKLEGMANAWSKYMMDNKVFEHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKSVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
BASE COUNT         5580 a         3557 c         2019 g         7791 t
ORIGIN      
        1 agattatttt tctttattct agttttgtca atacttttat ttattttttt gttacattca
       61 attccaatcc ccatgttacc tccccaaagc ttattcaaat atatactaaa ctacatattt
      121 atctataaat ctatttgtat ataaacctgt ttaactacat aactttagaa caattacaac
      181 aaacacttca cttaaatata tatattctta gctaagaaat ttctttagaa atccaagctt
      241 atctagtata tcttgtattt tattgacttt ttgacttata gaatctatag ctttttcaat
      301 tgaatctact ttagcctcta aatcttcaac ctgattaact tttttatcta tagtaccatt
      361 attagttata tcactattag atgaattatc tataagtttt cctatatatc catcattaat
      421 caattttgta tcattagcag aagctaatat catcaacgta tctatatctg aatttctagt
      481 attttctata actctaccat ttttgtcacg aactgacaat atagtgattg tatcatggtt
      541 attaaatcca ccttctccaa tatttctaac tataacattt accaaagtag catagttatt
      601 cacttcacca ttaggcatat ctactgtaag agtctttgaa caatcaccct ttatattcac
      661 agttattggt ttatttgtta ccattctaaa agcttcattt acagtattct cttttggttt
      721 aaagtttata atatcattag gacttgctat atttatcata tcagttaatt cctcaacagt
      781 ttttgcttca aacacagttt tttcttgtag acttttaatt tcatcaaaag cttcttcatt
      841 tccatttcca cctacctgtg ttatagtatt taatttttta gtactaagta tgtccctttg
      901 ttttgtgttt aatttattac caaccaaaac aacaggagac tcattttttg cagccaatac
      961 tccaacagct aacgagtcta ttagttgatt ctcattttta ctaccatcct tagttacata
     1021 taaattactt aaatttgtat cagtgtaaaa cttttctatg accttagcat tagtctcatt
     1081 tctatctttc ccactcactc tttctgcatt aggaagactc ttttctacag ctcttgaaac
     1141 agaatttgta cctccaatta catatgcttt tataactttc tcatccctta taaatttatc
     1201 aaatgcttct actccgtcct taggactaga caaaattata ggcatcttat tttgagctgc
     1261 tggtgcccca acgctaacag catcactcaa tcctttttca ccatttacta cagcaacttc
     1321 cttaatatct tttatatcct taagcttatt agccaaaatc agtgaagttt catatctatc
     1381 cttaccatta attctttcta cattaagtcc attacttttt aacttgttct ctatatcttt
     1441 attaagtaca gcagtacctc ctatcaaata aatatcttta gctcctagac gttttatctc
     1501 tttttcagtc ctatcatcta gcttatcact ttctgtcaaa agtattggtg ctcctttagc
     1561 ctttgcaaat ggtgtagctg aaagtgcatc agctaatgaa ttgtcattaa ctagcactac
     1621 attaggacta gaactgtatg ccttttgact tatctttaca gcagtttcat atctaccatt
     1681 acctattaat ttttcctctg taagtgcata tgatggtatt atagaaatgc tacttaaagt
     1741 aagcgctatt gtcagtattg ttaaaatagt ttttggtgct ttcattatta ttccccctta
     1801 ttttgtttaa aatcttacta tatttatatt ataaaataac tttacactat tttacagttt
     1861 aaaattatta catttttcat caataataag aaaactatct aaaaatatag acaatcctat
     1921 ttttcattat ttaaatgata aaaatataaa acaacaaaaa agagtgcctc atgaactaaa
     1981 aagttcattt tgagacaccc cgttttgtta ttttatattt ataaacttct attgaatatt
     2041 ttttaataat aaaatgtata tccttactat ctttccattc actattttcc taaaagagta
     2101 tttagttcat taaaagcatt ttcatttcca tttccaccaa ctctaatagg agtttctatt
     2161 ttcttagact taagtacatt tttttgactt tcatctaact tatttccaac taaaactact
     2221 ggtgaatcag ttttagcacc taatgctcct actgctagtc catctatcaa gtctccttga
     2281 ctctttgaac catctttaac aacaaaagca tatttcaaat ctgagctagg atagaattta
     2341 tctattatct tagcattagt ttcatttcta ttacttcctg caagtcttgt tggatttgaa
     2401 agcttacttt ctaaatttga tgataaagta gctgttccac caattatata agacttagtt
     2461 attttagatg aatttaaaaa ctcatcagca ccttgcaact cactcttttc attagtaagt
     2521 attattggca tattgttatc agcagctgct gcaccaacac ttattgcatc tgcaagtcca
     2581 tttacaccat ttgccactgc aacttgtgaa acagatttta tactatttaa cttttgagct
     2641 agagatatac tggtcttgta tctatcacta cctgaaattc tttctactga tatacccata
     2701 ttactaagtt gagtttgtac attcttgctt aaagaatttt ctccacctat tagataaact
     2761 tttttagttc ctaatctttg taactctgcc ttagtttttg aatctaagtt atcttttcct
     2821 gtcaaaagaa ttggagagtc aattgctgct gtaaatggtg tagctgttaa agcatccact
     2881 attgcatctc catttactaa tactgcattt tgtgaagaag tccatccttt ttgactaact
     2941 ttaactgctg tttcatatct atctgctcca gttaaagatt ctcctgaagt agcttgtcca
     3001 gaagatgttt ttgttatagc ttttataaca aagttacaag gagaaaattc actatatgta
     3061 tttatatctt caaatttatt gttttgaagt atgtagcttt cacctaagtt tgctttagcc
     3121 tcatatagat agtatccatc tatatcagtt tcatatgcta aagttgattt ttctctatta
     3181 ggatttgctg tgttgtctat aactactact attgctcctt taccttttgg taagctgtaa
     3241 gaattagtag gtacatttat gtatccagaa tatgatactg ttccacttgc aagttttgtc
     3301 attgaattgt tttgaggaac tccatttact actggtgcat aatatacttc atattttgct
     3361 cctacagaat ctgtttcaaa cataacagag tcaagttttt cagaatctct gctaaaatca
     3421 aatacatttg ctgctgttac tttatttgac attatcttgc taagaccagc atattcaagt
     3481 tggtacattt ttttatcgct atctggtttt gatactgatt tcattgcgta gttatctgta
     3541 tcttttaaaa gagttttgtc ttcataagaa atccagaaaa agcctttttc tgagttaaat
     3601 tctccccaac tactttttac taaccatgct ccatttgatt ctggtctaac acttggtttg
     3661 aacttatctt ttgaataatt atcatcccat cctacaattg aaacagaatg atttaaaggt
     3721 tcacttttat cattacaatt atacgaattt tcatcatcac ttaagtaggc tgaatagtgt
     3781 gcatatcctg atgtcactgc accatattgc attattgcat ttttaacaga agttttatct
     3841 ttactaattc ttacaacatc tgttacatta aattgtgtag gtgcagtatc catattttga
     3901 ggcctagttg ctccttcatc ttctgattta aaattatatg gaatgtcttt ttccagctta
     3961 ggtcctgccc aggctgtaag atatccaatt gcagttacat tcgaactacc tgtcatatca
     4021 gttaagttcc atccatgtat tcctccagtt gaccaccatc taaaatgctc ttctgaaaga
     4081 tcataagttc cataaccttt taatttcaaa tatgcttcta gtgttgacat tccagaaaaa
     4141 gcccaacatg tattaagttt tccttggtct tttgctggtg tagttaaatc taattttctt
     4201 ggatcatatg atacacttga tttatatgat gctggaagac tacttcttac attagtttta
     4261 agtgttgttc cttccatttc ataaggagtt ggtattactc cattatattt tgctatatct
     4321 ccatttttta catcttccaa gtattgaaga tatgattcag aatactctgc agtttctact
     4381 ccagtcagaa ttttattttt ttctgctgag acaggtattg ttgacacaat taaaaaacaa
     4441 actgtcaaca acaccagtag ctttgacaat ttttttaatt tatactttct catatttacc
     4501 cccttgtatg tatttaattt ttattatatc agatttctaa atctaggtca tattttattt
     4561 ttataaaaat aggaattata attatacata tataaaatac tattttatac tgcatattta
     4621 ttgattaata tgtggtatac taattacata ataatatata ccaaataatg gaggaaaatt
     4681 ttttgatatt aaaaaaacat ctagaaacaa tattatatct tttttttggt gctttaacag
     4741 ttttaataaa cattattact tacctatttt ttacaagaat tattaatttt acttttttat
     4801 tatcaaactc tctatcttgg tctatagcag ttttattttc atatataaca aacaaatttt
     4861 ttgtattcaa aagtgaaaaa actgagtttt tatttctatt aaaagaattt gtatcttttt
     4921 tatcttgtcg tttattttct ggttttatag aaatattact tatgtattta atgattgatt
     4981 tattttcttt aaatgatatc ttagtaaaaa taggcactaa tataattgtg ataattttaa
     5041 acttcgtctt cagcaaggta gtagttttta agcatagaaa ttaacattat tatatttatc
     5101 tttatgcaga ataaggagtt gcctaaaaaa aacaactcct tattagcttt aagaaacaat
     5161 ttctatttta tctgttgctc cccatttttc aactttatta ttttctcctg atgctaattc
     5221 tacataaagt ccatttacct tatttccttc taaaattata tcactattag aagtactttt
     5281 ccaactggag tctatttcat ctcctacatt catagcttct ggttttgtag tctttattct
     5341 atagtaaact ttattaccat tgtatccagt tacactaata gttactttag aattttcatc
     5401 aacagaaaca gttgatttta ttccatttgc tactatttta aaaccatcat ctgtcgcacc
     5461 agctcttccc catcttgaaa ctttctttct tgtataatca atttcaacaa cttctatata
     5521 tgatccattc ttagcattta cttttaatgg ttccgtatct tcaaccaagt cccaatcatt
     5581 tatgttaatt ctatcaccaa ccctcatagc tgatggttta tatgacaaaa ctctataata
     5641 aattttataa tttgaatttt taggtttctg aggtacagtt aacataattt gattattctc
     5701 aagaggagaa catgcactta tctttggaga taatcctgct gctgtatcac tatcatcatc
     5761 tttatctgta ttattagtgt taggtttatt gccacttatt cctggttctt tatttccact
     5821 aacagtatca atctttcccg aatttcttat attagtactt gaagaatcat tatctatctt
     5881 tgttatagtt ccatcatttc ttatatttac atcttttgca gaactaaata ttgttacaat
     5941 ccatatttgt ccattatttt cattttcaat cttgcaacca ttatcatcag aaatgtccat
     6001 ttgatttata tatcctgaat tagttaaagt tccatctttt atatctttaa gtatcaatgt
     6061 tttacttata tcacctgtat tattcaactc tccatttggc atatttatag ttattgactg
     6121 actatattct ccatataaat caacctttat agctttttca gtctctaatt taaaagattc
     6181 acttattttt ttatcaggtc taaactttat aatatcatta gcatccactt tctttaaagc
     6241 agaatccaat tcttcaattg tttcaactgt atatttagtt tcttcttgca tatccaatat
     6301 atctttatag acattttcat ttcctattcc accaacctga gtaactgtac taaatatctt
     6361 agtatttagt acatctttct gaatagaatc aattttattt cctactaata ccactggaga
     6421 attgtttttt gcagccaaca ctccaactga tagtgaatct attaaatcac tttgtttttt
     6481 cattccatcc ttggttatat atgcatttct taaatctgtt tctttataaa attcttttat
     6541 aacattagca tttgtttcat ttctattatt tcctgatatc ctcttagaat ttggtaaatt
     6601 tttttctatt tcattagaaa tggaatttaa tcctccaata atataagaat tttttatatt
     6661 ttgggctttt ataaatttat tagctacttc aactccaagg tttgaatctg aaagtataat
     6721 tggcatgtaa ttttgagctg ctattgaacc aacacttaca gcatctgcta atcccttttc
     6781 tccatttact acaattatat cagaaatatc tttttcatta ttcaattttt cagccaattt
     6841 taaggatgtt tcatatctat cattcccaaa aattctttca actgttattt tctctgattt
     6901 taattcattt tctacattta cacctagtgc atttgaacct cctactaaaa atacattttt
     6961 tactccaagc ctttttagtt ctgattttgt cctaaaatcc aattctttac ttccagatag
     7021 taaaataggt gcatctttca atttagcaaa tggagttgca gataatgcat ctgctattga
     7081 gttatcattc actaatacag cattatcaga tcttaaccaa ccgttttggc ttatttttat
     7141 tgctgtttcc catctacctg aacctattaa actgtcttta ttagatgcat ttaccacatt
     7201 actaggtaaa acaattgata aaaatgctgc tgttaataaa gatattattt tttttgatat
     7261 ttgcattaat tatctcctcc taatttaaat ataattttat ggagcatgta catactgtgt
     7321 tttatttaat tctaaatcat caaaatgaga ttttactgaa tggtatccta tagcataatg
     7381 tccactttca ggattccatt tcttatatgc attcatcgca cttaataact tctcctcttg
     7441 actaggtaat accttccaga cttctacatt tggattaact tttttaaaat ccttatagtc
     7501 atatggttct acataaaatc ttaaatcttt tatttttcct tctctatata actctagtgc
     7561 agcttctcct aatgctctat gatcttcatg tattccacta gctttaaacg gagtaacagt
     7621 ttttactttt gcttctggat attcttctaa gtatttaagt attatttcct ttgccttttc
     7681 tttgcttaac tcaccatcat gagctctatt atcttctata tgtatattac tttcttttac
     7741 acccattgct tcacagctat ccttaaattc ttcatctcta tcttttgaaa attcactttc
     7801 tgataaagag tatttatgaa catctttaat atgataagaa catgaccctc catcatccaa
     7861 aacttttctt attatagatt tactaccatc tgtgcacaat atgacatgtg tatcactttt
     7921 ttcaacatgt tcaattatag cacttcccat acttaaggtt tcatcatctt gatgtggtgt
     7981 aaaaaacaaa gttgcatcta ccactactac atcctcctac tttataaaat gttataattt
     8041 acaataaata gcataattat agaaaaagat tcctgtatta attaaggaat cttctccaca
     8101 gtataagtta ttaccaacct agtattttag ctatcttttg tattactgtc aattcaactc
     8161 cattacctat ttgagttact tctgcaccaa gatttaattt tttactaagt gcattctttt
     8221 gtgagtctgt tatatcagtt ttagctagaa gtattggacc tttacctgct gctagaggtc
     8281 ctgctgccaa tgcatctact agcataccat tatttccata tccatctttt gctatatata
     8341 gtttatctaa tttgccataa tatgtttcta taactttagc attagtatcg tgtcttgttt
     8401 ctccttcaac tctttgaact tttctatctt tatcaatatc tgcaagttga ttttcaattt
     8461 ggctagatac attattagag ccaccaacta tgccaatctc ttttccatcc atcaatttga
     8521 tagcatctgc tgatagatca ttccaaccat ttactattat aggagcctta agttcagcag
     8581 ctttagcagc tatactcata gcatcagcct ccccttttgc gcctactaca aatgcatctt
     8641 taaaagaccc catagctttt gctactgcta cagaagtcat atgtctatca tctccagcta
     8701 gtctttcaac attcttagtt actgattcta attgcttttt agctgtattt gatactgctg
     8761 attctccacc tactatatat attttagctg atgggctttt cttaattata tcttttatat
     8821 aatctaaagt tacttttggt atttcattat cagatgctaa taatataggt gcttttttag
     8881 attgagcaag tggtgtagct gctaatccgt caactagttt atttgaatta actataacta
     8941 tattgtcagt atattttgtt tgttttgcta tagccactgc agttgcatat ctatcatctc
     9001 cagctatcaa tttaaccttt gaattacctt ctaatgcagc tttggcatct tttaaatctt
     9061 tttcattggt actttctaca gtaagctctg ttgcttttcc agatgcatca gtatacttaa
     9121 tagtaaattt atattttcct gtttctgaat cttttgctga aatatcacta attgactttg
     9181 atgtaccatt tgctaccata ttacctttaa cagtcaatct atatccatca tataaatctt
     9241 gtggtgaaac tgtatttagg tcactatttt ttatagttat ttcggccaat tggacatcat
     9301 caatattttt agctcctgct acacctagtt tatcgatttt atttactcta aatccttctg
     9361 cagaagggtc taaatttgtt ccagttgaat caactggatt tgctgttaaa tcaacttttt
     9421 catcaccaat acctacatat atagttttag aagttactgt ttctgtttta tctcttaaag
     9481 ttataactaa cttatcttta gcactatcat atgaagcttt tacatctgca actttataaa
     9541 ttccattggt ttcagtttta gctttttctg ttatagaatt agcctcagcg gaaaggtctg
     9601 ctgaattttc atatttttca gttgcttctg caactacttc atttgcacta tttgttatat
     9661 gtcctttatc tgttatagtt acaataagtt ctttattttc tcccaaagca tttactaatt
     9721 tctctaattc atctatatta gttattattt ttaattttga taaagtctca ccaactttta
     9781 tttcatatat tggttgtcca acttgagaac ctcctgtata cttttgagac attaaacctc
     9841 taactttcga aactaattct gtagcctctt tcttagttat tgtttctttt tttacctgtg
     9901 tagtttctgc agcaaaaact ggagctactg atgtacttat cattgcagca gccataatta
     9961 cagaaagatt ttttttattc atggtatttt ccccctaaaa aattcaaatt tttattattt
    10021 tttccttatt taccaattat aatatatatt tgatattatt tcaataaatg agacttattg
    10081 taataattaa ccaaaagata taaacttttt cttatatttt atttttttaa tatttacgta
    10141 tacaaaaaag taacaaaatt gtaatttttt ttaataatcc agtttataac taatagactt
    10201 ttcatcttct ttatagtata atattaaatg gaagaaagaa atttcaagca tctctttttt
    10261 ccgtatttct cacatagaaa aagaggctga ttatcagcct ctttttccta tacagtttaa
    10321 atttcttaat taaatttata taaatactgt atagtcgtct ctcttatatt tttatttaat
    10381 gattcaaaca tctcataacc ttccatagca tattctttga aaggatcttt ttgagcatat
    10441 gatttaaatc ctacatattg ttttaattgc tccatagcat ctatatgttc tacccaatat
    10501 tgatcaacta cttttaaaag aacaattttt tccatttcgg ttattttgtc aaggcctact
    10561 accatctttt ttaaatcata aactcttttt gatatttcat aagtactatc aattatatcc
    10621 tcaatacttt tattatctaa gtcaggtatt agtaaggtat cagctggcat aaatgtatta
    10681 taaagatgct taaaatatcc atagtaatcc ctctttcttt ttgttaaata ttcagcagca
    10741 gcttctttta ttatatcttt aaccatgttt tgaatatcta cttgtatatc ttcatcattt
    10801 agaactttat ttctttcgtt atagattact tttctttgtt catttatagt atcatcatat
    10861 ttaagaacat ttttccttaa ttcaaaattt ttaccttcca cccctttttg agctctctct
    10921 attgcctttg taagagattt actttcaata gaattatttt tatttgaatt tggtcgtttc
    10981 attagtttct ctatactttt tccaccatac aatttgatta catcatcttc aagactaaca
    11041 aaaaaccttg atgttcctgg atcaccttga cgtccagaac gccctctaag ctgattatcg
    11101 attcttctag attcatgtct ctcagttcct attacataaa gtcctcctaa ttcctttact
    11161 ctactttctt cttctttatc acctgctcct aaagcaatat ctgttcctct acctgccata
    11221 ttagttgcaa tagttactga acccaactta ccagcctttg acactatcaa tgcttcttta
    11281 tcattttcct tagcatttag aacttgatgt tttattcctt ctttcttaag cataccagat
    11341 aacatttctg atttctcaac agaaacagta ccaataagaa ttggctgttt tgttttatgt
    11401 atccttatta cttcttctac aacagcttga tacttttctt tctctgttct aaacacttta
    11461 tcatgaagat ctgctcttat aattggtttg ttagtaggaa tttgtacaac atttaattta
    11521 taaattgatt caaattctcc ttcttcagtt ttagcagtcc ctgtcatacc agaaagttta
    11581 ttatataatc taaagaaatt ctggaatgta attgtagcca tagttttaga ttcatctttt
    11641 atttctaccc cttcttttgc ttctatagct tggtgtaagc catctgtata tcttcttcca
    11701 tccattactc ttcctgtaaa ttcatcaact atcattactt cttgattaac aacaacataa
    11761 tcaacatctt tttccataag tttgtgaccc ctaagagcct gatttatatg atgatataat
    11821 tctatatttt ctatatctgt aagattttta atcccaaaaa atgattctgc cttatcaata
    11881 ccactttcct ttagagctac ggtcttatct tttctatcta attcataatc ttcttctttt
    11941 attgttttta taaatgaatt tgctaattca taaagttttg aatcttcatc accctctcca
    12001 gcaattataa gtggagttct agcttcatct ataagaattg agtcaacctc atcaactatg
    12061 gcaaaattca actctctttg tactttatca aactttttag ttaccatatt atcttttaaa
    12121 taatcaaatc caaattcatt atttgtacca taagtaatat cacaattgta ttgaactttt
    12181 ctaacttcag gtataagatc tgcaataatt actcctacag acatacctaa aaactcatac
    12241 actggttgca ttagctcttt atcacgttgt gctagatagt catttactgt aacaacatgc
    12301 acacctttac ctgttaaagc atttagataa acaggtgcaa cttctaccaa agttttacct
    12361 tcaccagttt tcatttctgc tatttttcct tgatgaatta caatgcctcc aattaattga
    12421 accctatatt gacgcattcc taaaactctt tttgaagctt ctcttgccac tgcaaatgct
    12481 tctggtaata aactatctaa agtttctcct ttttttaatc tatctctaaa taggtctgtc
    12541 atttttttta attcgtcatc agacatattt tctatatctt tttctaaaga ttctatttta
    12601 tcaactatca cattaagcct tcttatttcc tgctcatctg ctttatccaa tattgtatcc
    12661 aaaactgaca tatttaattt acctcctttt ttatcataca attactatta taccttaaaa
    12721 ttatagaata gtagacaatt atttttattt atagtataat aatcatactt aaataaatta
    12781 catctttaat tatttaggta tttctcacaa aaaaaggaac tatttttagt tcctttttta
    12841 atttcttttt ataaaaaaga gaacttcttc aaagaagttc cctcttattt atagtttaat
    12901 tttatcttat aattctaata aatcttttat tctcttaata actgaatctg ctattccttt
    12961 accaacttga gtcaatttct tagaatcatc agaatttgta actttactta ttgctactga
    13021 ttggtcagaa gataaactat cagttgctaa aactattgga gcttcattgt gacctgctac
    13081 tgctgctatt gctaacgcat ctactaattg atcttctttt gtagaaccat ctttagcaac
    13141 atagaagttt ttaacacctg tgctaattac actgtcacta ttatcttttt caaaataagt
    13201 ttttataact tctgcattag tatcttgtct atcatctcca ctaactcttt ctggactctt
    13261 acctgtagca tcatctatag catcttccat gtcttcagaa acactagatt ttccacctat
    13321 tatatctaca taagcacttc ctataaagtc ttttgcatca gttgataaat cttttccttt
    13381 tccatcaact actactattg gcatttcttt attagaagca actgatgcta tactcattgc
    13441 atctgctaat ccagtaccac caactacaaa tactttatta tggtttaaac ctatttcatc
    13501 agcaatttct aaagatgtag catatctatc atcaccagat aatctttcaa cttttaatcc
    13561 catatctttt aattcatttg ctacttcttt agatacagaa ttttccccac cagctatata
    13621 aacagtcttt ttagatgtta taccagtttt atcatctaat cccattactc tctttatttc
    13681 agattttact gatgaatcta acttatcttt tgaagttaat aataatggag catctttaac
    13741 agcagctaaa ggtgaagcta ctagaccatc tactattgat tgtgatccta ctaatactac
    13801 ctcattagct tctacataat ctcctttaat tcctccatct gcattataag agtctttact
    13861 tatttctatt gcagtctcta ttctatcatc accagctaat acagaattat ttgaataaga
    13921 attattataa gttcttaatt catctaaagc tgattttaag ttattcttat tagtagatct
    13981 taatgttatt atagcaggag tatctactct atcacttgta ggtaattctg gcttatagtt
    14041 agatgatgca tatgttgaag ctgtatttaa tcttttacct gttggataaa gaactacctc
    14101 ataataatca ttgtcagttt tttcaaaatc acttgctgtt actgtcttat atgcatcttc
    14161 taattcagta tctttaaata catatttttt agctaaagct tctgcttttt cttttgcatc
    14221 tccagatatt gtaactgttt catctgatgc tgctataact gttacatttg attcaactgc
    14281 tccaactttt tctccagttt tttcaaaact taattttgat gtaccatcag tagtattaac
    14341 cgttataact ggtttactaa aatcatatat ctctttagta ccatcaagag atgctactgc
    14401 tgtatctaaa gtaggtgcta ctgctgcttg tgtatcatat tttaatttaa ttgcaccagc
    14461 tactgcatca gcatatgttg ctgtaatagc tactgttcct gtagcattat taccagtctt
    14521 atcagtaaat tttgaagttt tgactgctgc ctttgtagct gctaaaactt catctttagt
    14581 agctccatct acataagctg acgtagttgt atcatatttt tgtaacttta atttagcatc
    14641 attttctatt gaatttccat cttgatctac aatcttaact gttgttgcag taccaacttt
    14701 agtaactaca gcattactat caagttcctt taataatgta tcatcatatt ttttttctgt
    14761 tccatctgta atttttacag atatttttgc atattctcca gcttttaaat ctttaatttc
    14821 atctttaaca taacttattt gacttgcttc taaacttgat atagataccc cttttggatt
    14881 tccttttttg tcaaaaaagt taactgttat gcctgttaca ccatctgcaa catatttttt
    14941 aagtattctt tctatatcac tatacttact actagataca gtggtatttc catttggaaa
    15001 actatcctct gctgcagcga aaactggtgc tgctgatccg attactgtaa cggcagacat
    15061 tacaactgcc aaatttttct tattcattcc ttaaattcct cccaacatta tattaattac
    15121 atctctttaa ttatgtacat taaaattcca tgttcatata aaccaaatat ttctcacact
    15181 cttatgatag actatataaa atagaatttc aataatttat gcaaaattaa tagaattttc
    15241 tttcaacatt tttccccttt tttttgctat ttcatatttt ttaaattaaa ttttcaattt
    15301 taattttatt aatacattta tttatctgat ttaatcaata tatattaata ttttctacca
    15361 taaaatatta atatatactc taactattta aaaattccat tgtagcatta acttctcttt
    15421 gattagaaac atacattcca aagccaaaac tataaaactc ttcctttaac atattatcat
    15481 tatacttaga agacttcttc cataaatcaa atacagactt agcaatctct tttgcatctt
    15541 tagatgtata tacagactta acattcatag gtaaatatgc aatattttca tcagttctca
    15601 ttccaaagcc agaaaatact tcagctgcat ttttcccatc tatttcatga gcaaatatct
    15661 tcttctcatc catatactta gaccatgcat ttgccattcc agctaaagaa tctaattctc
    15721 ttaaactttt cttacctttt tcttttctat atgaattaac tagattatac atttcattag
    15781 ataccatagc cttaaactca taactattag ggtcttttgt acttgtgttt ataattactt
    15841 tgacatttac attaatctca cacttttgcc caaaattatc aaaagcactt acatttaaaa
    15901 catacgttcc tgccttactg gtatccacag ttccctcaat attaggcttc aaattatttc
    15961 cataataatc agtggcaact atattcaaca tactattatc aaacttatcc cctacagaaa
    16021 tctctacatc attagcagtg attgtaggca ttgtattttt atcagatgca gaactaatac
    16081 attggtcagc aaccttctta tccattccac ctaaagaagt aacttcatca gcacctctaa
    16141 gcacactctt atcacttcca tcattaacaa gtactatagg tttgtcctta gccaacggag
    16201 aaccagccac agcatcaact aacttatacc cttgagaaac ataaaacttc tttgttcctt
    16261 tataaaagcg ttgtataacc ttcttattag tttcaaatct atctttacca gcaatcctaa
    16321 cagaattagt cttactcaca agttcattac tcattatctc ctctgaccca agagaataac
    16381 attgaacgcc atcaacctta aatggtatac tctttccatc agtaagtata acaggaactc
    16441 catctctagc agctacagaa gatacactca ttgcatcagc ctcacctgtg tatccattta
    16501 caagaaaaac cttatcacca tcatttactg gtttaatagc tgaaatctcc ttagctataa
    16561 gatagctagt ctttattcta tcttccccac caattctttt aacttcaatc cctttattct
    16621 taagttgatt ttctacagat ttacctattg tatcctctgt acctataaca taagcattct
    16681 taacatcctt tagtcttttt tcaacatctg ttggtatctt atctctttgc actaacatta
    16741 taggagcttt tacaactcca gataatccac ttgcacttaa tccatctact atagaattat
    16801 cagtatttac taatatgact gtatcatata tttgtttatc tgctatcaat gcagaagtct
    16861 catatctatc tttcccttgt atcttttcta tactcgaaag tgcattgata tttggtgctc
    16921 ctgccattat taatgatatt gtcaatccta ttgataatac tctcttattt accttcatcg
    16981 aactcctcct actaattttt ttatataatt cagctaatca taaattacac cctcagtagt
    17041 gattccctca aaatttataa actactaaaa caaaaaacac ctaaaatacc ataacctgta
    17101 aagcaataat aaaacagatt acaaaaagta ctttagatgt gattttcatt ttataatatc
    17161 agtaaattaa caaaaaaaca agatttatgg aattttttac aagctacctt cattgccatt
    17221 taaaaactct tgagtagcat gcacttgacc atcagataat atataaagcc caaatccagt
    17281 actataaaac tcatcactaa gcatgtttgc attgtattct ggagatttct tccacacttc
    17341 aaatatagcc tttgccaagt cttttgcatc ttgagttgtt tgaacactct tagaatctat
    17401 ataaatatat gctatatttt cctcacttct cattccaaac tcagaaaata cttgtggagc
    17461 atttttgcca tctatataat gttcaaaaac tttgttatcc atcatatact tagaccatgc
    17521 atttgccata ccttctagct tactagatac taccaaaggc tctttacctt tttcttttct
    17581 ataggaattg attaagttat acatttcagt agataccatt tttttaaatt ctgggctgtt
    17641 gtaatcatga gatttatcat ccaacacttt tatttctact ctttttcctg tactttttcc
    17701 ccattcgtct gtagctttta atgtcaatat gtatgtacct actctatttg tatctataaa
    17761 cccatcaact tctattggta ataccttccc agtgttatct ttagctacta tatttaactt
    17821 gccagtgtca aacttttcac ccttatatac ctctgtactt ccaactgtaa ttgctggggg
    17881 ttgtccattt gactttgaag cacttataca ttgttgtatt accttctcat ctatttcacc
    17941 tacagaggtt atattcttag ctccttctaa tacagtttta tcgcttccat catttactag
    18001 taccattgga gagtttttag taagtggtgc agctgctatt gcatctacta attggtatcc
    18061 atctgataca tagaatccat ctgcactatt aaaaaagtag tctattacgt ttttgttagt
    18121 ttcaaatcgg tcagttcctt ctattcttac tgagtttgta tttttaacta gaggattact
    18181 cattatctcc tctgaaccta tacaatatga ttgaatattt tttacatcaa aaggcacact
    18241 ctttccatct gtaagtatga ttggagctcc atctctagta gctactgatg atacactcat
    18301 tgcatctgct tctccactat atgcattagt tagtagtact ttatcaactt tttttatagt
    18361 tgctatttct ttagctatta aatagcttgt ttttagtcta tcttctccac caattctttt
    18421 gacttctata tttttagaat ctagttcttt ttctactgat ttacttatag tatcttctgt
    18481 acctattata tatgcttttt tgatattttt tagacatcta tttgtgtctg ctggtatctt
    18541 attttgttgt gtaaatagta ttggagcttt tgtagctccc gataatccac ttgcacttaa
    18601 tccatctgca agtgattttt ctgtatttac taaaattact gtttcataag tctgtttatc
    18661 agctatcttt gctgctgttt catatctatc tttaccgtat attttttcta ctactgatga
    18721 tgcatttaca cttttgaagt ttactaagat taatgatact gctagaccta atgataatat
    18781 ttttttgttc attttcatat ccccctgatg atttttatat tttatattta tattgtgatt
    18841 attttccatt tattttctct ttattttcca tatattatag agtatatatg atatgaatat
    18901 atctcgtcta tatattagtg ttttatttta ttaaatttaa aaaatta
//
LOCUS       DAYASV010000069        18861 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYASV010000069 DAYASV010000000
VERSION     DAYASV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18861
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.069"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            89..1375
                     /locus_tag="ACGI68_002326"
     CDS             89..1375
                     /locus_tag="ACGI68_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0138761.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1487..1960
                     /gene="grdA"
                     /locus_tag="ACGI68_002327"
     CDS             1487..1960
                     /gene="grdA"
                     /locus_tag="ACGI68_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_110943085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1619..1621,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HFH0138762.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            1992..3302
                     /gene="grdB"
                     /locus_tag="ACGI68_002328"
     CDS             1992..3302
                     /gene="grdB"
                     /locus_tag="ACGI68_002328"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3039..3041,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HFH0138763.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3558..5090
                     /gene="grdC"
                     /locus_tag="ACGI68_002329"
     CDS             3558..5090
                     /gene="grdC"
                     /locus_tag="ACGI68_002329"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HFH0138764.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5101..6246
                     /gene="grdD"
                     /locus_tag="ACGI68_002330"
     CDS             5101..6246
                     /gene="grdD"
                     /locus_tag="ACGI68_002330"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HFH0138765.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6556..7635
                     /locus_tag="ACGI68_002331"
     CDS             6556..7635
                     /locus_tag="ACGI68_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0138766.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7748..8347
                     /locus_tag="ACGI68_002332"
     CDS             7748..8347
                     /locus_tag="ACGI68_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138767.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8533..9405)
                     /locus_tag="ACGI68_002333"
     CDS             complement(8533..9405)
                     /locus_tag="ACGI68_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0138768.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9822..11225
                     /locus_tag="ACGI68_002334"
     CDS             9822..11225
                     /locus_tag="ACGI68_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138769.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11252..12796
                     /locus_tag="ACGI68_002335"
     CDS             11252..12796
                     /locus_tag="ACGI68_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HFH0138770.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12827..14218
                     /locus_tag="ACGI68_002336"
     CDS             12827..14218
                     /locus_tag="ACGI68_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFH0138771.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14411..15907
                     /gene="abfD"
                     /locus_tag="ACGI68_002337"
     CDS             14411..15907
                     /gene="abfD"
                     /locus_tag="ACGI68_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HFH0138772.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16042..16317
                     /locus_tag="ACGI68_002338"
     CDS             16042..16317
                     /locus_tag="ACGI68_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138773.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16341..17648
                     /locus_tag="ACGI68_002339"
     CDS             16341..17648
                     /locus_tag="ACGI68_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HFH0138774.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVREHLAVEFEKRFKEKY"
     gene            17668..18786
                     /locus_tag="ACGI68_002340"
     CDS             17668..18786
                     /locus_tag="ACGI68_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HFH0138775.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
BASE COUNT         7226 a         2156 c         3734 g         5745 t
ORIGIN      
        1 ttaaaaaaat aagataaatt gtaaaatgat ataattttga gaaaaactca aatgcatata
       61 cacaaattaa ataacaggag gtgcaagtat gcgtcttgaa ttagggaaaa tctttataaa
      121 agatattcaa tttggtgatg taacagaagt aaaagatgga gtattattca tcaataagca
      181 agagatgcta caagaaattg gtggagatga gcatattaaa tctatagata tagaattagc
      241 tcgtccaggt gaaagcgtaa gaataacacc tgtaaaagat gttattgagc caagagtaaa
      301 agttgaaggt aatgggggaa tcttccctgg tatcatgtct aaagttgaca ctgtaggaga
      361 aggaaaaact catgctttaa aaggtgtagc tgttgtaact actggaaaaa tagtaggttt
      421 ccaagaaggt ataatagaca tgactggaga aggagctaag tacactccat tttctaaatt
      481 aaataatgta gtagtaatag ctgaacctat tgatggttta aaacaatacg ctcatgaaaa
      541 agctgttaga atgataggat tcaaagcagc aatgtactta ggagaagtag caagaaatct
      601 tactccagat gaagtttcag tatatgaaac taaacctctt cttgaatcaa taaaagaata
      661 tccagagctt ccaaaagtag gatatgttta catgcttcag actcaaggtt tattacatga
      721 tacttatgta tatggagtag atgcaaaaca aatagtacca actttattat atccaacaga
      781 attaatggac ggagcaattg taagtggtaa ctgtgtttct gcatgtgaca aaaacccaag
      841 ttatgttcat ataaacaatg gggttgttga agacttatat gcaagacatg gtaaagatat
      901 aaactttgta ggtgtaatta taactaatga aaatgtttat ctagcagata aagaaagatc
      961 ttcaaactgg acttctaaat tatgtaaata tttaggatta gatgcagtta tagtttcaca
     1021 agaaggtttt ggaaatcctg atactgacct tataatgaac tgtaagaaga tagaaatgca
     1081 aggtgttaaa acagttatag ttacagatga atatgctgga cgtgatggag gttctcaatc
     1141 acttgcagat gcagatgtaa gagctgatgc tgttgtaaca ggtggaaatg ccaaccaagt
     1201 tgtagttctt cctaagttag ataaagttat tggacattta gaagtagtag atgtaatagc
     1261 tggtggttct gatggaagtt taagagctga tggaactatt gaagttgaaa tccaagctat
     1321 aacaggtgca actaatgaaa caggatttgg tcatttaact gctaaaggat attaagaagt
     1381 attttattaa acgattgagt aatgttaata aaacatatta tagataatta taataagtgt
     1441 tatagatata taaaaatata ttagaaatat aataaggagg attattatga gtttacttag
     1501 taataaaaag gttcttataa taggtgaccg tgatggtata ccaggacctg cgatagaaga
     1561 atgtgtaaaa acagtagaag gagcagaggt tgttttctca tctacagaat gctttgtctg
     1621 aacagctgct ggggctatgg acttagaaaa tcaaaacaga gttaaagatg ctgctgataa
     1681 attcggagct gaaaatgttg tgattttact aggtgctgct gaagccgaag ctgcaggtct
     1741 tgcagccgaa acagtaactg ctggagatcc aactttcgct ggaccacttg ctggagttgc
     1801 cttaggatta agtgtttacc acgttgttga ggaaccaata aaatcattat ttgatgaaag
     1861 tgtatatgag gaccaaataa gtatgatgga aatggtttta gaagttgaag aaatagaaga
     1921 agaaatgtct ggtataagag aagaattttg taaattttaa tgcctaaaag caaaaggggt
     1981 gaaactaaga gatgggaaaa ctaaaagcag tacattatat taatcagttc tttgctggta
     2041 taggtggaga agaaaaagct gatactaagc cacatgtggc agaaacttta cctccaataa
     2101 gtttacaact tgataaatta ttaggtgaag atattgaaat agttggaaca gtagtttgtg
     2161 gagatagcta tttcaatgaa aatatagata gtgcaagtga agaagtttta tcaatggtaa
     2221 aaggttttga accacaactt ttcatagctg gtcctgcatt taatgctggt agatatgggg
     2281 ttgctgctgg aacaataact aaagttgtaa aagatgcttt gaatatacca gctttaacag
     2341 gtatgtatgt tgaaaatcca ggagcagata tgttcaaaaa ggatgtttat gtagtagaaa
     2401 cttctgattc tgcagcaggt atgagaaagg cacttcctaa gatagctaaa cttgcagtaa
     2461 aacttgctaa tggagaagaa attggaactc ctaaagatga aggatacata gctagaggaa
     2521 taagagttaa ctacttccat gaagacagag gttctaagag agctgttgac atgttagtta
     2581 agaaaataaa aggtgaacca tttgaaactg aatacccaat gccaaacttt gatagagttg
     2641 acccaagtaa agctgtaaaa gatttatcaa aatgtaaaat agctttagtt acttctggtg
     2701 gtatagttcc aaaaggaaat ccagatagaa tagagtcttc ttcagcttct aaatatggaa
     2761 cttattctat agcaggagtt atggatttaa cagaagagac ttatgaaact gcacatggtg
     2821 gatatgaccc agtgtatgca aatttagatg cagatagagt attacctgtt gatgttctta
     2881 gagatttaga aaaagaaggt gtaattggaa aattacatga gactttctat acaactgtag
     2941 gtaatggtac atctgttgca aactctaaaa aatatgcttc tgaaattgga gcagcattag
     3001 tggctgatgg tgtagatgca gtaatattaa cttctacatg aggtacttgt actcgttgcg
     3061 gtgcaacgat ggtaaaagaa atagaaaaaa ctggacttcc agtagttcat atgtgtacag
     3121 tagtacctat ttcattaaca gtaggggcta atagaatagt tccaactata gcaatacctc
     3181 atccacttgg aaatccagcg cttgacccta cagaagaaaa agccctaaga agaggccttg
     3241 tagaaaaagc attaaatgct ttaactacag aagtagatgg tcaaacagta tttgaaaaat
     3301 aaaaataaca cacacatata ttatatagat aaaaatataa acacacacta aaaataaaaa
     3361 ataactatac acaaaaaaat aatatagatt tcatgaatgg ataaattgga gttattcatt
     3421 catgaaatct taaaaaatgc caaaatacat aaacaataat agaatcaaat atacaataaa
     3481 atacaatgat tagagctttt aataagttta ataagattaa aaaataagtg ttcataaaat
     3541 ttatggaggt gcacaaaatg acttatccag ttttaaaagg ggcaggctat gtacttattc
     3601 atactccaga tatgatagtg caaaatggaa gtacttgtac agttgaaaga gcaacaaatc
     3661 cagattcaga atttttaaaa gaagtaagta atcatataag aagttatgaa gatgtagtta
     3721 actacatgcc aaatcaagtt tatataggaa atagaagacc agaagaatta agagatttac
     3781 caatgccatg gtgtgaacaa aagattgagg gaactagaaa tggtaagttt ggagaaataa
     3841 tgcctcaaga tgagttcata gctttaatgc aaataagtga tgcatttgat ttagtaaaat
     3901 tatcacaaga atttattgat gaagtaaagc caaaaatcga aaataactac cctgaaattg
     3961 caccatttgt aggtaaatta aagggtgatg atatagaaga aggtaaagaa ttagtagcaa
     4021 cacacatagc tgaaggatta taccatgatg gaaaatttgt tggttatgta aaaagagcac
     4081 atgatgtaga tgttaactta aatgcccata caatgtttga aaatctagta gtaaaagcat
     4141 ctggagtttt atctgctatt caaatgctaa gacatagcaa aatagaccct gctgaaatag
     4201 attatgttat tgagtgttca gaagaagctt gtggagatat aaaccaaaga ggtggaggaa
     4261 actttgctaa atcaatagct gagatagcag gacttcaaaa tgcaactggt tctgacacaa
     4321 gaggattttg tgcagcacca actcatgcat taattcaagc agcagcttta gtaaaagctg
     4381 gtatacataa aaatgtaatg gtagtagcag gtggagcaag tgctaaactt ggaatgaatg
     4441 ctaaagacca tgttaaaaaa ggtcttcctg tacttgaaga tgttgttgga ggatttgcag
     4501 tacttgtatc tgaaaatgat ggtgtaaatc cagttataag aactgattta acaggtaagc
     4561 atactgttgg aactggttct tcaccacaag cagttatgac agcacttata acttcaggtc
     4621 ttgatagagc taatttaaaa ataacagatg ttgatgtgta ttcagttgaa atgcaaaacc
     4681 cagatataac taagccagca ggagcaggag atgttccaga agcaaactac aaaatgatag
     4741 gggcacttgc tgttaaacgt ggagacttag aaaagaaaga attgaaagat tttgtttcta
     4801 acaaaggatt accaggatgg gcaccaactc aaggacatat accatcagga gctccttata
     4861 taggattttt aatagatgat ttaactacag gaaatagaaa tagagctatg atagttggaa
     4921 aaggtagttt attcttagga agaatgacta acttatttga tggagtatct tttatagctg
     4981 aaagaaatac aggtgttact gaagagactt caggaatatc taaggatgaa attaagaaga
     5041 taatagctga atctatgaaa aaactagcat tagatatgtt agaagaatag gggtgaggat
     5101 atgtcaaaaa aagtaatcgc agatgtattc ttagaagtag ctaatgcaat agaaagcggc
     5161 gaatttggca aaaaagtaaa aattggtgtt actacacttg gaagtgaaca tggtgtagaa
     5221 aacatggtta atggtgcaca attagcaaaa tctaatttat ttgatattgt gcttattgga
     5281 ccaaaagtgg aaactgacct tgaagtagtt gaagttaatg atgaaaaaga aatgcatgca
     5341 aaaatggaag aattattaga ttcaggatat attgatgctt gtgttactat gcattataat
     5401 tttccaatag gtgtatctac agtaggtaga gtaataactc cagcaaaagg aaaagaaatg
     5461 atacttgcaa caacaacagg aacaagtgct actaatagaa tagaggcaat ggtaagaaat
     5521 gccatttatg gtattgctac agctaaatca atgggtaaca aatgcccaaa agttggaata
     5581 cttaatgttg atggagcaag acaagttgag aagtgcttaa aagaattaaa agataatgga
     5641 tatgacatgg agtttgcgga ttcaattaga gcagatggtg gatgtgtaat gagaggtaat
     5701 gatttacttg taggtgcacc tgatgttatg gttactgata cattatctgg aaatatattt
     5761 atgaaagtat tttcttcata tactacaggt ggagattatg aagctcaagg ctttggatac
     5821 ggacctggag ttggagagga ctatgataga aaggtactta tagtatcaag agcatcaggt
     5881 tctccggtag tagcaaatgc tttaaaatac gcttatgatg ttgtaaaggg agatataagt
     5941 aatgttgcaa gaaatgaatt tgcaaaggtt aaaaaagcaa aatttgatga cattatatcc
     6001 tctcttacta aaaaagaagt taaagctgaa aaagtagaag ttaaaatgcc agataaagaa
     6061 attgttactc gtcaaatagc tggtgttgat ataatggatt tagaagatgc agtatctgag
     6121 ttatggaaaa acggaatcta tgctgaaagt ggtatgggtt gtacaggtcc aatagtttta
     6181 gtaaatgaag ctaaaggaga cttagcagtt gaaactttag ttaaagctgg atatactgca
     6241 aaataagtac attttttata tgtaaataag aacatatagt tataatttta ttgattataa
     6301 ttatatgttc tttttatttt atatcttgtc aatatttata tattaacaca atccatttat
     6361 ttgattctct agctaattta ttatatttat tcagttaaaa ttaagctttc taactaaata
     6421 aaaactaatg aagtattaaa atgaaattga taaaatatga aaaaatttta ggaataaagt
     6481 atctgttaga taaaaataag attaattagt gatataatat aattaagata aaatttatta
     6541 tttggaggca aaatcatgag cagagtaaaa aatgtagtag aattgttgga aactaaaggt
     6601 gttgatgcac tatatctaac aaagaaaact aatgtaaact atataagtgg ttttcctgat
     6661 gaagaagcat atgcagtgat ttgcaaagat ggaaactttt tagttactga tagtagatat
     6721 atggagcttg cagaaaaggt ttgcaaagat tttgaaatta taaattggca taattttgat
     6781 agaagtgtgg caaaggcagt taaaagtgta tgtgataaag tgggtataaa gaaacttggg
     6841 tttgaaagaa ctaatatagt gtttgataaa tatgaagaac ttaaaaatct gattgagaag
     6901 gataatggag aattaatacc tacagaaaat attgtagaga ctttgagata tgtaaaagac
     6961 aaagatgaaa tcaaaaatac aagaaaagct tgtgaaatag cggataaggc attagaagaa
     7021 ttgattccac atataaaagc tggagttagc gaaattgaac tggctacaaa gttagagtac
     7081 tttatgaaaa tgaacggagc acaaaatata gggtttgaaa ctatactgat atcaggagca
     7141 aagacttcat tgcttcatgg taagcctagt gataagatta tagaaaaagg tgattttgta
     7201 cttatagact atggcgctat gtataatggc tatatatctg atacaactag aacttttata
     7261 gttggaggag cttctgaaaa gcaattagaa atatataatt tagttaagga agctcaaaat
     7321 gtaggggttg agaacatgaa agctggtgta catgcgacaa ttccagatgc tgagataaga
     7381 aaagttgtca aaaaatatga agactattac tatcaaggta taggacatgg agtcggtaga
     7441 gatgttcatg aagaaccttt cattggtaat tatggtgata aaatcataga agagggatgt
     7501 ataataacaa tggaaccagg tatttatttc cctggatggg gtggagttag aatagaagat
     7561 actgttttga ttactaaaaa tggtccagaa agacttacta aattccctaa agatttgatg
     7621 attttagaca aataatttaa tttatatata tttttttaca taaaatataa cataatctca
     7681 ctattaagct ctatttacat attatagtaa agagggctta attttattgt gagaggtgat
     7741 agtatatatg tataaaaaac ataaatttat aaaaaatata aaaaaagcct ggtataaaat
     7801 caaattaatt gatagatgcc ttataattgt aatggcaata ttaatgtttc aatctatata
     7861 taatttattt gttaatgaag taaattcaca agatactaca actattgatg tagtagtaag
     7921 aaccacttca gcagctatat ttgggtattt tttaagtgct aattttataa agagaccttt
     7981 aaggaagtat acaaatactg atgtatcttc aaatcttttt atagataata atttatcccc
     8041 aaaaggtcaa gagtctaatc aaaataatat aatgaatgtc aaaaatacca ttggatttac
     8101 ttcagaagct gataattatg aaaagaaaat acctattaat aataatgaag gttttgaaga
     8161 aggagaaact agtgaattgc aaattattat agctactgta atttgtatag tagcattaac
     8221 agtacttttt gttgtaagaa attttacaac tacaacatca gcttctttag gtactatttc
     8281 acagatgaga gattttgtct caggatgcgt gggttttttg ctaggttgtc caagtaaacc
     8341 tgattaaaaa agtattatca ataaaaggta ttttaaaaag tatcagtata ataatattta
     8401 gatttaaatg tatacaatta taattctaaa cattctatat tgtattttta tactgatact
     8461 ctaaactata ctatttatta aaatatttta acattgtatt taaacaaaat attatatctg
     8521 tataatataa atttagttta tatgaataga tgccatatac tcaataaaaa gtttcatact
     8581 atgagatatg tattgatttc ttctccaaag aaccccaata ttcgcagtaa ttgcacgttc
     8641 taatggtatt gaacagagat tttctgtatt ttgaaatatt tctttataca atattgttga
     8701 agccaagtca ttttctagta aataacgtat ggtagaaagt tgatttgagt gtaaaattat
     8761 atttggctta gctttttcaa gtctttttgt aataatctta tagtgataag aaccttcaga
     8821 taagattaca aaaggtacat tttttaacat ataattcgat ataatgggat gttttgccaa
     8881 ttcatttttg ggatttgtaa ctaagtataa ttctgtttta tagattgtct ttgaatctat
     8941 atcatcataa atatcactgt caattatacc aatacaaaaa tcaagtgtag attcttcaat
     9001 cattttagct ccaacaaatg ttggaacttc aaaaatttta agctctatat ctggataaat
     9061 tacattaaat ctaggaagta tttttgaaag caaaaacgta cctaaaactg taggaatacc
     9121 tattttgaga gaagcttttc tcttttttcc taaatcaagt gcgtcacatt gaaaatcctc
     9181 aaattgtttt ataaaatgtt ttgacttgtc taaaaaaatt tttccttcag gcgttaactc
     9241 tattttatta cctattctgt aaaaaagttc aaagccaagt tctttttcta attctttaat
     9301 tgctaatgtt atggatggtt gagaaatatg aatactttcc gcagccttag tcatgttttt
     9361 atatttacag acagtttgaa aatagtataa atagtttata ttcatttggt attagttcct
     9421 ttcatttaca tattagtgac aagtatataa ttaacaatag cataagtttt gtatattgtt
     9481 gtaaaaaaat agaatagttt taaactatca tagcataagt ttttgatatt ttcaatctat
     9541 taagaatagt gctattatta aattaacaaa agagaatagc tggtatggtt tagagtaaat
     9601 caatctatat tacagttata tcaacgaata tcaaaaaatt atctattaga aaataaaaat
     9661 tatttttaca attaaattgt taattaaatc ataaagaaaa aagtgatatt aacaagataa
     9721 aattttttta atcagaaatg ttaatttaat aacaaaatag agtattgggg agaaaggaga
     9781 aatatatttt ttaataatat ttgttaattt aataacgaaa aatgaaaggg agtgatttag
     9841 tgaagtttga attgatggat tttttaatga atccttttgt ccttatgttt gctgcagtaa
     9901 ttacaggaat tttatttgga aaaattaaat ttgggaagtt taattttggg gtatcaggag
     9961 cattatttac aggattattt attggatggc ttgcatatag cttggggaat ctaataatag
    10021 caaaagggga aacagcagca ggatataagg cagcaactgt aatgatgggt aatggaatta
    10081 tttcttctga cttctttgat ttctttttaa taatatttgt tgcagcagtt ggtctattgg
    10141 cagcaaaaga tatgaaagct gtattaaaga agtatggagc aagatttgta attttaggag
    10201 ttcttataac ttttataggt ggctctatga cttatgcaat gactttacta tcaagtgata
    10261 aaggaagtag tgcttatgaa gtttctggcg tttacacagg agcattaaca agttcgcctg
    10321 gattagctgc tgctttagaa acagctggta aacatgcaga agatgtatca aaagaatttg
    10381 aaaaagcttc aataaaagat aaaaaagaaa ttttaaaagt agtcgaccca gaaggcaaat
    10441 tggatgtgaa tacaactaca tcactgactc aagagcaaat agataagtat attgcatatg
    10501 ctgaagctgg tgttggtata ggtcatgcag tcgcatatcc ttttggagtt ttaatagtaa
    10561 tcttaggtgt aaatttcctt ccaaaattat ttaggatgga cctaaaagaa gaaagaagaa
    10621 aatatgagaa ggaaatgaag gaagcaagag atagtgtttc tggtaaaacg ataccagaag
    10681 ttccatttaa tataataaca tttttcttga catgtttagc tggatactta gttggaggaa
    10741 tacatgtatt tatgggacca ctagggtact tcacactagg cgcaacagga ggttcactaa
    10801 ttgtatctct agttcttgga tatataggta agataggggt agtaaatttc agaatggaag
    10861 aaaaggtttt aaatatttta aaacaaatag ggctagtatt tttcttagca atagttggat
    10921 taagatatgg tggaaaagta gttgattcaa ttatgacatc tggtatgcac ctagccttag
    10981 tagcaatagc agtaggtgtt acagctatga tgataggatt cttagttggc aaatacgtat
    11041 ttaaattaaa ttggatttta ttatctggag ctgtctgtgg tggtatgaca tcaacaccag
    11101 gtcttggagc tgctgttgat gctcttgata gtgatgaccc agcagcaggt tatggtgcta
    11161 cgtatccatt tgcattatta actaaagtta tattggttat agttttacac aaacttccaa
    11221 tgtaaataag attttatatg gggggatatt tatgaataat gaaggagcat ttgaagaatt
    11281 aagtggaaga ttaagaaata aagaattgtg tgatttaata atgaatgcag atgaagctgc
    11341 taaaattata gaggatggca tggttattgg agttagtgga tttacacctt caggctatcc
    11401 aaaagcagta ccattagcag tttcagaaag agctaaaagt ggagaaaaga taaaattaac
    11461 cgtatattca ggagcatctt taggtccaga agttgatgga gcatggtcag aagcaggtat
    11521 aattgaaaga agacttcctt atcaaactaa ttcaatactt agaaataata ttaataaagg
    11581 tgttgttgat tatatagata tgcacctaag tcattctact caatttttaa attatggaac
    11641 tatacctaaa gtagatgtag ctatagtaga agcacttgca ataactgaag aaggaaatat
    11701 tataccaact agtgggatag gtaattctcc atcttttata aaaagtgcag ataaagtaat
    11761 agtagagata aatcttgcaa aaccaatgga aatggaaggt atggcagata tatatattac
    11821 ggaaaatcca ccaaatagaa aaccaataga aataaatcat cctaaggata gaattggaac
    11881 aacatatata ccttgtggtt tggataaaat tgcaggagta gtaattacca atatgcaaga
    11941 taagacccgt ccattgggag ttgtagatga agcttcgaaa aaaatatcaa ataatattat
    12001 tgctttttta agagaagaag taaaggcagg cagattatca aagaatttac ttcctcttca
    12061 atctggtgta ggaagtgtag caaatgcagt attatatgga ttatgtgaat ctgaatttga
    12121 aaatttaact tgttatacag aggttgtaca ggactctatg ttggatttaa taagaatggg
    12181 taaagttaca atggcatcta ctacatctgt aagtccatct ccagaaggat taataaaatt
    12241 tgaaaaagat attgattttt ttaaggataa gattatatta agacctcaag aaattagtaa
    12301 caatccagaa atagccagaa gaattggtgt aattgcaatg aacactgcaa ttgaagttga
    12361 tatttatgga aatgtaaatt ctactcacat aatggggtca aagatgatga atggaattgg
    12421 tggttctggt gattttgcta gaaatggagc aataactata tttagtactg agtcaattgc
    12481 aaaaaatggt gatatatcat caatagtacc tatggtatct cacgtagacc acactgaaca
    12541 tgatgtaatg gtaattgtaa cagaacaagg atatgctgat ttaagaggtc ttgcacctag
    12601 agaaagagct ataaaaataa tagaaaattg tgcccatcca gattataaag aacaacttag
    12661 ggattattta aatagagctt gtaaaagtgg agcaaaacaa acacctcaca tattggacga
    12721 agctttatca tggcatagta aatttatgag tacaggtaca atgaaaaagg ctgaaacttt
    12781 taaaagtgca ttataaaata tttaataaat tggaggtaga ttttaaatgg aaaaagcagt
    12841 ggaaaacttt gaagatttaa gtaaggaata cattaatggt tatattgaga gagcaagaaa
    12901 agctcaaaga gagtttgaat gttatactca agaacaagta gataaaatag taaaaatagt
    12961 tggaaaagta gtgtattata atgctgaata tttagctaaa cttgctgtag aagagactgg
    13021 aatgggagta tatgaagata aggtagctaa aaataaaagc aaagcaaaag ttatatataa
    13081 caatttaaaa gataaaaaat cagttggtat aattgatata gatagagaaa ctggaataac
    13141 aaaggtagct aaaccagttg gagtagtagc ggcaataact ccttgtacaa atccaatcgt
    13201 tacacctatg agcaatgcaa tgtttgcact taagggaaga aatgcaatta ttatcacacc
    13261 tcatcataaa gcaattggat gtagtacaaa aactgtagag atgattaatg aagaattaga
    13321 aaaaatagga gctccagaaa atttaataca aatacttgac caacaatcaa gagaaaatac
    13381 aagaaattta atttcatcag cagatgtagt aattgccact ggaggaatgg gaatggtaaa
    13441 agctgcgtac tcaagtggaa aaccagcact tggtgtaggg gcaggaaatg ttcaatgtat
    13501 catagataga gatgtagaca ttaaagaagc agtacctaaa atcatagcag gaagaatttt
    13561 tgataatggt atcatttgtt caggtgaaca gtcagtaata gtagcagaag aaatgtttga
    13621 taaaataatg gatgaattta aaaataataa aggttttata gttagagata aagtacaaaa
    13681 agaagctttc agaaatgcta tgtttgtaaa taaatctatg aataaagatg cagtaggtca
    13741 atcagttcat acaattgcaa aaattgcagg tgttgagata ccagaagata caaaaataat
    13801 agtaatagaa gctgatggac caggagaaga ggatattatt gcaaaagaaa aaatgtgtcc
    13861 agttatatct gcatacaagt ataaaagttt tgaagaaggt gtagctattg caaaagcaaa
    13921 tttaaatgta gaaggaaaag gacatagtgt atctatacat tcaaatacag ttaaaaatat
    13981 agaatatgca ggagaaaata tagaagtatc aagatttgtt attaatcaat gttgtgctac
    14041 tagtgctgga ggaagtttct ttaatggtct tgcacctact aatactctag gttgtggttc
    14101 ttggggaaat aacagcatct ctgaaaatct tgattataag catctaatta atatttctag
    14161 aattgcttat tatatgccag aaaatgaagt ccctacagat gaagaacttt ggggataaaa
    14221 tttagacaaa aaatattcat tctaaattaa agtaggaata tattttaatt tagaatgaat
    14281 ttaagacata aattaaattt aaattagttt attggactaa atttaaaata ttaagagaca
    14341 tatagaagaa ggaagtatta taatttaaaa taaataaaaa aatctataaa agagaaggag
    14401 tggttttatt atggcattaa tgacaggagc tcaatatatt gaaagtttga gaaaattaaa
    14461 tactaaggtt tatatgtttg gtgaagaggt taagaattgg gttgaccatc ctatgattag
    14521 accatctata aattgtgtag cagcaactta tgacttagct catgacccag aatatgcaga
    14581 tttaatgaca gttacttcaa atatcacagg tgaaaaaata aatagatttg gtcatcttca
    14641 tcaaagtgtt gatgatttga taaaaaaagt aaaaatgcaa agactatgtg gtcaaaaaac
    14701 agcttcttgt ttccaaagat gtgttggtat ggatgcgttt aatgcagtgt attcaacaac
    14761 atttgagtgt gataaagccc atggaactaa ttatcatgat aattttgtaa aatacttaac
    14821 ttatatacaa gaaaacgatt tagtagttga tggagctatg actgacccta agggagatag
    14881 aagtctatca cctagtgctc aacctgaccc agatatgttc cttcatatag tagaaagaag
    14941 agaagatggg ataattgtaa gaggagcaaa ggcacatcaa actggttcaa taaattcaca
    15001 tgagcacctt ataatgccta ctataagtat gactgaagca gataaagact acgctgtatc
    15061 atttgcagtt ccttcagatg ctgaaggagt gtttatgatt tatggaagac aatcttgtga
    15121 cactagaaaa ttagaagaag gtgcagatgt tgatttagga aataaggagt ttggaggtca
    15181 agaagcttta gtagtatttg ataatgtatt tgttccaaat gatagaattt tcttatgtgg
    15241 agaatgggat ttctctggta tgttagtaga gagatttgca ggataccata gacaaagtta
    15301 tggtggatgt aaagtaggag taggtgatgt aataatagga gcagcagctt tagcagctga
    15361 ttataatgga gcaaacaaag cttcacatat aaaagataaa ctaatagaaa tgactcacct
    15421 taatgaaagc ttatattgct gtggtatagc atgttcatca gaaggtcaca aaacagaagc
    15481 tggaaactat caaatagatt tacttcttgc aaatgtgtgt aaacaaaatg taactagatt
    15541 tccttatgaa atagtaagac ttgcagaaga tatagctgga gggcttatgg ttactatgcc
    15601 ttcagaaaaa gactttaaat cagaccttaa agttggaaca agtggaatga ctattggcga
    15661 agtatgtaac aagtatttta aagcttcttc agtggcttca acagaagaaa gaatgagaat
    15721 attaagattc ttagaaaata tatgccttgg ttcatctgct gttggttata gaactgagtc
    15781 aatgcatggg gcaggttcac cacaagctca aagaataatg atttcaagac aaggaaacat
    15841 aaatcaaaag aaagaattag ctaagaaaat tgcgggaata aaaaaagagg aagctttaaa
    15901 tttataaaaa gttgatatat aaagggaaat tatatattaa tgtgtgttaa ttaatgaaaa
    15961 aatatgtagt ataagagata gttataaaat tatggtggtt tttagctaag taaagaaatt
    16021 tttaataagg agaatttaca catgacctgt ggagttagat tttgtggggg ctgtaaccct
    16081 agatacgaaa gagggaaggc tcttacaata atcaaagagc acttcaatgg tagggtagat
    16141 tttagtatag cacaagagaa cgtcgagtat gatttgttac ttataattgg aggatgcgca
    16201 actctatgcg catcctatca acaatataaa gtgaaaaatg aatgtgttaa gatgtgggat
    16261 tttagccata tagaaagagt tataaatgtg attgaatcag caatttgtga aacttaaaaa
    16321 agctaaatgg ggggatttca ttgagttggc aagaattgta tcaaagtaaa ttatgtagtg
    16381 ctacagaagc tgtaaaacag attaaaaacg gtgatactgt agtatttgca cattgtgtag
    16441 gtgaacctcc tgcactagta gaagctatga ttgaaaatgc tgaacaatat aaagatgttg
    16501 agattaaaca tatggtaagc ttgggtagtg gtggatatac tgctaaggga atggaagctc
    16561 attttagagt aaatccaatg tttgtaagtg gaaatgtaag aaaggcaatt gaaaatggag
    16621 atggtgattt tacacctgca ttcttccatg aagtaccaaa gttgcttaga gaaaaaagat
    16681 taaaatgtga tgttgtttta gcacaggtaa ctccaccaga tgaacatggg tattgtagct
    16741 taggaacatc agttgattat acttatgaag ccattaaaac tgctagaaca gtaattgttc
    16801 aagtgaatga ccagtttcct agaacctatg gtgaggtagt ccatgtatca gagtttgact
    16861 atatagtgga aaaatcacaa cctttatttg aactacaacc tgcaaagatt ggagaagtag
    16921 aagaagcaat tggtaaaaat tgtgcttcat taattgaaga tggctctact ttacagctag
    16981 ggattggtgg gataccagat gcagttatgt tatttttaac tgataaaaag gatttaggga
    17041 ttcattcaga aatgatttca gatgggactt tggcccttta tgaaaaaggt gttataaatg
    17101 gtaaatataa aaattttgat aaagaaaaaa tgacagttac cttcttaatg ggtactaaaa
    17161 aattatatga ctttgcaaat aataaccctg cagtagaggt aaaaccagta gactatgtga
    17221 atcatccagc aattataatg aaacaacata agatggtttc tataaattct gctatacaag
    17281 ttgatttaat gggacaggta gttgcagagg ctatgggact tagacaattt agtggtgttg
    17341 gaggtcaagt tgactttata cgtggagtgt ctatgggtga agatggaaag gcaattatag
    17401 ctatgccttc aataactaca aaaaaagatg gtacagtaat ttcaaaaatc gtatctattg
    17461 tggatgaagg tgcacctata acgacatcaa gaaatgatgt tgactatatt gtaacagaat
    17521 acggtatagc agaattaaaa ggaaaatctc ttagagaaag agcaagaaat ttaataaata
    17581 tagctcatcc aagtgtaaga gaacatctag cagtagaatt tgaaaagaga tttaaggaga
    17641 aatattaata atagaggggt gttaataatg gaagcactta gagtagttcc aaagatattt
    17701 tactttgata catttaaaga atttaatgaa gaatttaaaa taggaaaaaa tgatttagta
    17761 attactaatg agtttatata tgagccatat atgaaacctc ttggaataga tacaaattta
    17821 atttttcaag aaaagtttgg aacaggtgaa ccatctgatg aaatgataga ctccatgaca
    17881 aaggaaatga agaaatacaa ttttgataga attattgcat ttggaggagg aacaatagtt
    17941 gatatatgta agatattagc acttgatgtt ccagaaaagt ctattgattt atttgaagga
    18001 gaagtatcac ctaaaaaagt aaaagagtta gtagtagttc ctacaacatg tggtacaggt
    18061 agtgaagtaa caaatgtagc tattgcagag ttaaaatcta aacatactaa aaaggggctt
    18121 gcagtagaag aaacatatgc agactatgca gtgttaattc cagagacagt aaaagggtta
    18181 ccttacaaat tctttgttac aagttcggta gatgctctta tacatgctat tgagtcttat
    18241 ctttctccaa aagcaagtcc atttacagag atgtattctc tacaagcaat aaaaatgatt
    18301 atggatgggt ataagaagat agttgataaa ggagaagaag agagatttaa tcatcttaga
    18361 gactttgtac ttgcatcaaa ttatgcagga attgcttttg gaaatgctgg ttgtgcagca
    18421 gttcatgcac tatcttattc aataggtgga gctttccatg ttgcacatgg tgaagcaaat
    18481 tatcagttct ttactgaagt atttaagatg tattctagaa aaaaacctaa tggaaaaatt
    18541 ataaaatgta caaaaatttt agcagatgcc cttgaatgtg accctaactg tgatgtttat
    18601 ggagaattag aaaagttttt aaataaatta atagctaaaa aagctttgag agaatatgga
    18661 atggtggaaa gtcagataga tgaatttaca gatagtacaa tagcaaatca acaacgacta
    18721 cttgctaata attatgtaga gcttagtaga gaggaaataa gagagatatt tgcaaatctt
    18781 tattaataca attttttatt tatatgtata gtcaacataa acaatgctaa ttatatttat
    18841 gtacaaataa aaataaggga a
//
LOCUS       DAYASV010000070        18555 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYASV010000070 DAYASV010000000
VERSION     DAYASV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.070"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            143..1360
                     /gene="ilvA"
                     /locus_tag="ACGI68_002341"
     CDS             143..1360
                     /gene="ilvA"
                     /locus_tag="ACGI68_002341"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HFH0138776.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1455..1835
                     /locus_tag="ACGI68_002342"
     CDS             1455..1835
                     /locus_tag="ACGI68_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0138777.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2119..2601
                     /locus_tag="ACGI68_002343"
     CDS             2119..2601
                     /locus_tag="ACGI68_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0138778.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2622..3437
                     /locus_tag="ACGI68_002344"
     CDS             2622..3437
                     /locus_tag="ACGI68_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0138779.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3488..5038
                     /locus_tag="ACGI68_002345"
     CDS             3488..5038
                     /locus_tag="ACGI68_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0138780.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPFLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5135..6487
                     /locus_tag="ACGI68_002346"
     CDS             5135..6487
                     /locus_tag="ACGI68_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFH0138781.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6618..7376
                     /locus_tag="ACGI68_002347"
     CDS             6618..7376
                     /locus_tag="ACGI68_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138782.1"
                     /translation="MNIINKIFWEEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLIDNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELLNLLRTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7423..8142
                     /locus_tag="ACGI68_002348"
     CDS             7423..8142
                     /locus_tag="ACGI68_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HFH0138783.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8266..9666)
                     /locus_tag="ACGI68_002349"
     CDS             complement(8266..9666)
                     /locus_tag="ACGI68_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFH0138784.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADIGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9670..10560)
                     /locus_tag="ACGI68_002350"
     CDS             complement(9670..10560)
                     /locus_tag="ACGI68_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0138785.1"
                     /translation="MTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKATFYKHF
                     ETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWKNIFSRTTINTYI
                     RENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDALIHQFI
                     YIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYFSLHNKS
                     LFFKTIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAYLEKEDT
                     LRPKVLLLNSIKNQLKKETL"
     gene            10839..11486
                     /locus_tag="ACGI68_002351"
     CDS             10839..11486
                     /locus_tag="ACGI68_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0138786.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11811..14450
                     /locus_tag="ACGI68_002352"
     CDS             11811..14450
                     /locus_tag="ACGI68_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFH0138787.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHIEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMNTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14508..15611)
                     /locus_tag="ACGI68_002353"
     CDS             complement(14508..15611)
                     /locus_tag="ACGI68_002353"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0138788.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVREAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            15971..16792
                     /locus_tag="ACGI68_002354"
     CDS             15971..16792
                     /locus_tag="ACGI68_002354"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFH0138789.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16871..17082
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17169..18485
                     /gene="argH"
                     /locus_tag="ACGI68_002355"
     CDS             17169..18485
                     /gene="argH"
                     /locus_tag="ACGI68_002355"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFH0138790.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELQKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7077 a         2151 c         3297 g         6030 t
ORIGIN      
        1 tatgcaattg ttgtattttt aggttttttg tgattaatta aaaaattata aaaatcgttt
       61 acatgttgat ttttaaaaaa aaaagatata gaattatata gaaagtaata aacaaaaatt
      121 ggatttacgg gagtggaatg aaatgatgaa tgttacctta acagatgtga aagaggctag
      181 agagaccatt aagaacatag taaaaagaac tgacttactt gaaagtgtta aacttagcga
      241 aaaaacagga gcaaatgtgt tctataaatg tgagaatctt caaaaaacag gttcttttaa
      301 attaagagga gcttgtaaca aaatagcgag tttaacagat gaagaaaagg caaatggagt
      361 tatagcttca agtgcaggga atcatgctca aggagttgca ttgggagcaa aaatgactgg
      421 tataaaatct acaatagtaa tgccagcaac tgcgccttta gcaaaagtta gtgcaactaa
      481 aggttatgga gcagaagtag ttttaaatgg tgcggtttat gatgatgcat atgcaaaagc
      541 tgttgaaata caaaaagaaa caggagcaac tttcttacat ccattcaatg ataagtatgt
      601 tatagcagga caaggaacta tatctcttga aattttcgaa caattagaca ataaagttga
      661 tactatacta tgcccagttg gtggaggagg aatcatctct ggtgttgctg ttgcagcaaa
      721 agcattaaat cctaatgtaa aaatagtcgg tgttcaaact gcaaatatac cttcaatgaa
      781 agagtcaata aaaaatggaa aagtaacaac agcatttaat gatactacaa tagctgatgg
      841 tatagctgtt aaaactccag gagatttaac ttttgaaata ataaatgaat tggttgatga
      901 aatagtagtg gttgaagaga cagaaattgc agaatcaata ttatttatga tggaaagtca
      961 aaaaatagtt tcagaaggtg ctggtgctgt atgtacagct gctatattaa gtggtaagta
     1021 tgtaccagca aaagatgaaa atgtagtttg tataatatct ggtggtaata tagacataaa
     1081 tacgttatat agaataatag gtgttgcttt agctaaagaa ggaagaagat attcatttag
     1141 tacaattatg gaagataagc caggaaactt tgctgagtta actagaataa taagtgagaa
     1201 tggtggaaac attttaagtg ctaatcaagg taaattatca gcaggggaag ctctaggaaa
     1261 acaaagtgcg gaatttatat tagagacaat agattatgac catatagcta gaataaagaa
     1321 agctatagaa gaaaaaggtt ttaaaattat agaattataa taaaaagtgt atataatata
     1381 caagaaaaat agtataatag tgtatgaaga tacattttat gtgtattaga ttaacgaatt
     1441 aggaggattt taaaatgaaa catgaagtaa tacatactaa tgatgcacca gcagctttag
     1501 gaccatattc tcaagctata aaagctggaa acttgttatt tgtatctgga caagttcctc
     1561 ttgtaccaga gactatggaa gtagttgaag gagatgtaca agctcaaact gctcaatctt
     1621 taaaaaatct taaagctata ttagctgaaa gtggagctga tttttcaaat gtagttaaga
     1681 ctacagtatt tataaaagat atgaatgaat ttggggctat aaacgaagtt tatgctgaat
     1741 attttggaga aaataaacca gcaagagctt gtgtagaagt tgctagatta ccaaaagacg
     1801 ttaaagttga aatagaagta atagctgtat tataaaaata caatattttt aatttgaaaa
     1861 ggctgaaaaa ctatattcta gtgattcagc ctttttataa aaaaataaat cacaataaag
     1921 aacatgataa atattgtcga attaaaaaat aaaattaaaa taaatattgc aatatttttg
     1981 agaaggtttt ataatgagta tataatttga aacgtgtaac tattaaatta ggcatgagtt
     2041 cttggatatt tttatcctga ggattcatgc cttttttgta ttgaaattca aaaatctatt
     2101 taataaggag gaagtactgt gttaaatttt ttcaagaaaa ataaaagcta taagttacat
     2161 gcagtagtga gtggtaacag cattaatatt gagaaagtaa acgattctgt gttttcaaaa
     2221 aagttaatgg gagatggagt tgcaattatt cctaatagta atgttgttgt agccccttgt
     2281 aatggtaaag taactgtgct tacagaatca aagcatgcct ttggtatggt ctcagatgag
     2341 ggtgttgaaa tattagtcca cataggaatt gatacagtta gtttacaggg tgaggggttt
     2401 aaaaatgaag tgtctcaagg agatactgtt aaaaaaggta gtccaataat aagttttgaa
     2461 agagaaaaaa taaattctca gggaattgat tgtacaacaa taataattgt attaaatcat
     2521 agcgaatttt cagaaataaa ttgcatggta gaaaatgaag ttgttgcagg gcaagatact
     2581 gttattgaaa ttatgaaata ataggaaggg attaattcaa tatgagagta gtaaaaatct
     2641 ttaataataa tgcattatct acagtaacta atgataggaa agaagctatt ttactaggtc
     2701 ttggtatagg ttttaataaa cgtccaggag ataaagttaa tgaagataaa atagaaaaga
     2761 tttattatgt tcaagaccac atgcaaacaa aatttttaga attattaaaa aatgttagtc
     2821 cagaagtgat ggatgcttca caacaaatta taagtcttat accaggaaat gagggaaaat
     2881 ttaataataa agggatatta tctttagtgg aacatattag ttttgcaata gatagaatga
     2941 aaaataatgt ttttttacct aatttaatgc ttttggatat aaagatgatg tattcaaaag
     3001 agtttgaatt aggagtaaaa gctttagaaa ttatatatag agtatgtcat atccatttgc
     3061 ctgaagatga agcaggttac atagctttac attttgtcaa tttacaatca aatgataatt
     3121 tggcatatga tactttaaaa tttgtaaaag gtagtattga tttgattaaa gaatgttatg
     3181 gattagaatt agacgaatcg agtttaagta cattacgttt tagaactcat cttaaatttc
     3241 ttgctcaaag aatttttaag aatgaaattt gtcaggatga taaaatgatt gaaatgtatg
     3301 attatttaat taacaatcat cctaaaaata aagaatattt agaaaagtta aatgtatata
     3361 ttgaaaagga gtttaggtat aaattagata aacctgaaaa aatttatcta ctactacacc
     3421 taacaaaaat cttataagaa tgagatgaat tagtaataga aatatataat tttagggggg
     3481 gataagattg aaagaaaaac tacttggagg actggaaaca tttgcaaagg caatggttca
     3541 gccattaatg tatttatcga gtgcaggaat attaatggtg gtaggtgtac tacttacaaa
     3601 tgatatgtta ttaggagtac ttccattttt taaatgggag ccaattcaaa tatttggaag
     3661 cctaatttat aactgtataa tggctattat taataatctt gctgttatat ttgctatagg
     3721 tattcctgct gcactagcaa aaaaagagaa gcacaaagca gcgttaatag gatttatgag
     3781 ttatataata taccttacag cttccaacac tatgttaaca tcactaggtc aactagcaaa
     3841 agaaagcaag atgcttggac tgatagggac tggacaagct actattttag gtattcaagt
     3901 tgtagataca agtgtatttg gtggaattat tttagggtgt atagctggat atgtatttaa
     3961 tcgtacaagc aacaaaacat ttaaaggagc tttacagata tactcgggag caaattttag
     4021 ttttatatgc atgttctttg tatccatttt aattggagtt gtgagtaact ttatatggcc
     4081 attagctcaa caaggaatag cttctcttgc aaatgtcatc aaagatacag gtacatttgg
     4141 tttattctta tatggttttt tagagagaat attagtacca actggacttc atcatttaat
     4201 ctatacacca tttcagtttt ctgatttagg aggagtgctt aaagttggtg cagatacagt
     4261 ttcaggagcc tatgctatag ttatgacaga aatgaattta ccagttgagc gtttctcaga
     4321 tagtatatac tacatggcaa ctggatttac aaagatgttt ggttatatag gtataggagc
     4381 tgcctttatt tatactgcat ataagcaaaa taagaagaaa gttaaggcaa cgattattcc
     4441 actaataatt acggcagtat tagcctctgt aacagaacca ttagacttta tgtttgtatt
     4501 ctgtgcacca tttttattct ttgtacatgc agttatagct ggtcttttta tagctttatt
     4561 aaaaatattt gatgttactg cattatgtgg agggaactta ttagtatcaa ttataatgaa
     4621 tactacagta ggcgtagaaa aaacacactg gccagtaatg atgatattag gacttgcaca
     4681 aattattgta tattttgtag tatttagttt tttaattaag aaatttaatt ataaaactcc
     4741 aggtcgtgaa gatgagagta cagctacagg cgaagaacaa tcaaaacaac ttaatttaga
     4801 tgcagggata gaaaatataa ttgatgggtt aggcggaaaa gaaaatatta atacagtaga
     4861 aaattgcatc actagattac gtgttaatgt aaaagatgaa agtaagatta atgaagatat
     4921 aattaattta acgccaaata gcgggattgt aaggaaagga aaagatatac aaataatttt
     4981 tggacttcat gtgcatgagg taagacgtgc agtagaagat tttttagaag cttattaaag
     5041 atatatattt atatagcaag ggtaaataac agctttaagt gctgtttact atttattaaa
     5101 gatacatata atttatagag gaggaataat tattatgatt attactattg ttggtggagg
     5161 aagcacattt actccaggta ttgttaagtc aattgcatta agagaaaaag agttagagct
     5221 tgaggaaatt cgcttatttg acattgataa agaacgccaa gataaagtag ctgtagttgt
     5281 caactggata ctaaaagaag atgttaaaac agatgttaaa ctaactgtaa ctaattgtga
     5341 aaaagaagcg tatacagatg ctacatttat atttgcacaa atgcgtgttg gtaaatatga
     5401 aatgcgtgag caggatgaaa aaattccatt gaaatatggt tgtgtaggtc aagaaacttg
     5461 tggttgtgga gggatggcat acggactgcg tacaatacaa ccaatgataa aggttattga
     5521 tgatgtagag aaatatgcta aaaaagatta ttggatatta aattactcta atcctgctgc
     5581 aatcgtttct gaagcttgtc gtaagttaag accaaatgca aggattataa atatctgtga
     5641 tatgccaata gctattatag atatgatagc ggggtcatta aatatcaatg acgttcataa
     5701 tatacgctat gattattttg gtctaaatca ttttggctgg tttacttcaa tagattataa
     5761 acatagagac ttaatggaag agattaagga atacataaaa gaaaataaaa tattattacc
     5821 aaaatcttat atagaaaact taaataaaag tcgtagtaca ggaaatcgcc atgctgtaag
     5881 tagctggata catgtttggg aatctgttta tacaatgtta gaaagcttcc caaaatatct
     5941 gccaaacact tatttaacat actatctttt aagtaaagaa acagtggaac attcaaacat
     6001 aaatcgtact cgtgcaaatg aggttatgga aagccgtgaa aaagacttat ttgaaggtgt
     6061 gagacattac ttagaaactg gagaaatttc tgaaaaagca ttttatgcag gttcacatgg
     6121 agattggatt agtgatttgg cagtttcaat taaaaatgat acacgtagtc gttttttagt
     6181 tataactgaa aatcgtggag ctataccaaa catgccatat gatgcaatgg tagaaattcc
     6241 agcatatatt ggtaaagatg gtccagaagt tattgctcga ggtccaatac ctctatttca
     6301 acaaggatta atgatgcaac aacttaacag tgaaaaattg atagtagaag cttatataga
     6361 aaatagttat gaaaaagctt tacaagcatt cacattaaat aaaactattc caagtatgaa
     6421 tgttgctaaa gaaattttgg atgaaatgat agaagttaat aaggaatact ggccagaatt
     6481 aagatagtag gataaaggaa agggttttta tttaataatt agatatgttt tcagagtctt
     6541 ttatcaaaat gtattcacaa acatatggca atatattaaa ataaagagtg atatttttag
     6601 aagaatgggg tgatgcaatg aacattataa ataaaatttt ttgggaagaa gataggattg
     6661 gcatgattac aaataattta gaggcagata cacatagcca ctgtatgtta caactgttct
     6721 tagggataga agacagcatt gaaataacag tcaatgaaaa attagttaaa tgtaattgta
     6781 tcatagtaga taaaaatatc tctcattcat tttcagcaag aaaaaaagtt tattactctg
     6841 ctattattga acctacatct atatatgcag aacagcttac ctcaaaaatg aatgattttg
     6901 gctactggat atgtgacaat gatggattag agaaattacg tcaacaagga acttttttga
     6961 tagataactc tagtaaagaa caatatttaa gatttatgga aatgcttaat aactatttga
     7021 atatacctat aactctcaaa cattatgatg acagaattac tgaattgctc aatttattac
     7081 gtacttgtaa ttgtgataat catactattt caagttttgc agataaagtg tccctatcag
     7141 caagtagatt atcacatctg tttaaagaac aaatagggat acctttaaaa agctatatct
     7201 tatttcatca aatggagtgt gcttttagag aattactttc tgggaaaaat gttaccgaag
     7261 cttctatgac tgctggattc gatactccat cacactttgc agggacagtt aagcgaatga
     7321 tgggaatgcc tgtttcttta tctttgaaag atagcgaatt tttgaaagta tattaaatat
     7381 tctcgttata taatgaagtt gtactaagaa aggggctttg ttatgaataa agaatattta
     7441 gaggaaacaa aaatgttaaa ttataatgaa ccacaactta agcttttggt atcatcaaag
     7501 aattggttgg aattagatga ttttcataaa ataaaatcta tttatgagtt tgtacagaat
     7561 gatattttat ttggatataa tgcttctgat atgttaagcg caacacaggt attaaatgat
     7621 ggtatgggac aatgcaatac aaaagctact cttttaatgg cattattacg tgcagtgaat
     7681 atcccttgta ggcttcatgc ttttgatgta acaaaagatt ttcaacgagg agctacatcc
     7741 aaattgattt cactattagc accaaaatat attcttcata cttgggtaga ggtattttat
     7801 caagataggt ggattgctct tgagggtgtc ataactgata aaaaatattt agaagcaatt
     7861 cagaagaaat tttttaatca tggtggaaca tttaaaaagt atgcaatagc tacaaatgat
     7921 ttgaaaaata catctattga ttgggatggt aaagatactt ttatccaaaa agaagctatc
     7981 gtgtatgatt atggtatttt tccatcacca gacgtatttt tttctactca ttcacaacat
     8041 atgtcaaaat taaaaaattt catatatgta catcttatca gaaaaataat gacaaaaaat
     8101 gtatgtaagg ctagaaacaa ttatattgat aaaaacgaat gagtgtttaa gaggtaacta
     8161 tataggaaat atatagaata attaaaactg tactggttaa aatgaataaa aaggagttat
     8221 cacagagcaa acttaaattg ttcataggat aactcctttt ctgtattaac ttctaataat
     8281 ttgatgtctt tttataaaac atatagatac aattaaacca gtaaatgcaa gagtagctgc
     8341 tattgaaaat gcaacatcta ttccatgaat gttagacagc atgtttgaac tcatatgagc
     8401 tttcttagta gcacttgtca ttatagtaat aagtatagca gaacctatag ctccagatat
     8461 ctgacggagt gtgttattaa ttgctgtggc atgagatata agttctctat ctaatgtttt
     8521 aattccccat gtagttaatg gcattaaaac cattgaaata ccaatcatac gaatacaata
     8581 catcaatgac acatgaatta aagatgtatc tctgcctaaa aaagcgaagg ataaagttcc
     8641 taaaaacaaa catcctgttc ctaatattga tagagcatga ggtccatatt tatctaaatg
     8701 acgtcctgca attggattga gtacaaccat caatatagca ccaggtaaca ttaatgaacc
     8761 tgaagataat gcagaaaatc ctcttacaga ttgaacgtac agaggaatca ttaatgtagc
     8821 agacatcatt gaggcatata caatcataat taatattgta gaaataagaa atggtttatt
     8881 tttaaaaacc ctcaattcta aaaaaggttc tttagatttt aattgacgca atgtaaataa
     8941 aattaatgac ataattccta ttaatattgg taaatatgtg gcaatattag tccacccata
     9001 attaccttga ttagtaaatc caattaataa accaccaaaa cctagagatg atagtactat
     9061 tgaaggtata tctaatttta gtttttgagt ttccccaaca tttcttaata gaataaatga
     9121 tagtataaca tctaatatag caatagggga aagaaacaga aataaactat gccaaccaaa
     9181 tgaatctact aaccatcctg ataaagttgg acctattgct ggagcaaatc ctactgtaat
     9241 accaactaaa ctcatagctg caccacgttt ttcctcaggg cataaataga gtataataac
     9301 ttgtaaaagt ggcattagag aaccagaacc cattgcctgc aacacacgag caatcaacat
     9361 agttgaaaaa ttattagaaa aaagtgcaat aatacatcct atagaaaaga tagacattga
     9421 tgtaataaat aactttctag tactaaatcg atttattaaa taagctgttg tgggtacaat
     9481 aatacctgca ataagtagat aaattgttgt taaccactga ccaatatcag cagtaatttt
     9541 aaaatctgac attatattag gtaatgctgt tgacaatagt gtttgactga gagaacttat
     9601 aaaagcacca gtcattacaa ttgctattat taaatttcct tttttattgt tactttgggt
     9661 ttccataatt tataacgtct ccttttttaa ttgattttta atgctgttca ataaaagtac
     9721 ttttggtctt aaagtgtctt ctttttctaa atatgcaatc attgcatcca taattaaaga
     9781 atcatattgt tgattttttc cttcgacgta taattttttt attgcttcta gtaatttagg
     9841 attttcaagg agagaagtat cagttttgat tagttcttct atatcttgta tcgttttaaa
     9901 aaatagcgat ttattatgaa gggaaaagta aacttcatct tcttgtttat gtaaaaacat
     9961 tgatttttca attaaaggat ttagattatc catattttct acaactaaat ctagaatttg
    10021 tatagtaaat ttggcaataa agtatacatt tatttctctt ttttgtacat gaataatgta
    10081 tataaactga tgaattagag catccaataa gaaaactaaa tcaaatataa ttttttctat
    10141 tttatcacca tagttatcaa ataagcttag tttgtattca ttcaataagt tagctcttga
    10201 ttttgtttgc aatttacttg tagccttacg ttgctcttca gaaaagtttt ctcttatata
    10261 ggtatttatg gtagttctag aaaaaatatt tttccataca gcaattatct ttctgtttaa
    10321 cttttctgat gatggaatgt cattattttc atttatggaa tgtataattt ctaaaaactt
    10381 ttcatcataa taattgatta tttcgcagat aaaactttcc ttagtttcaa aatgcttgta
    10441 aaaagtagct ttagacattt tacactcttt tacaacatcc gatattgaag tattttgaaa
    10501 acctttttgg tcaaaaattt tttgtgcaat agttataatg agcctttgtt tttctgtcac
    10561 tttattatct cctttcacgc aaaaacgttt agtactatat agtacctaca agttttgata
    10621 aatatattat acatcatttg gaaaaaaaat caatatatag actgtaatat gaaatttttg
    10681 aaaaatttgt tgttattttt agtcgtataa aaaagaaact taagagtttc ttaagaattg
    10741 tttaaagaag ttgcaataaa ttagtggtat gattatagta tgaaagagaa tgtcttgaaa
    10801 tttttctgta tcaaattcca attatggagg tgtagtatat gaaaaagaaa atttgtgtag
    10861 caataatttt tgctctattt atagtttttg tagaaatgtt tattgatagc tctagcgaag
    10921 gaagtaaaat tatgacagct gaattaagta aatacaggaa aggcgtaaat gtattatcaa
    10981 taaacaatat tatagtagcg aataaaaaat atagtttgcc caaagattat tctcctcagg
    11041 aaagcagtga agcgagagat gctttttata aaatgaataa agatgctcaa aagtcaggat
    11101 taaatttaaa agcttttagt acatatagaa gttatgaata tcaagataga ttatttaagt
    11161 catatgtaaa agaacatgga gaaaaagaag caaatagatt ttcagctaaa cctggagaga
    11221 gtgagcatca aactggactt gcttttgata ttggaggaga tgaccagtct tgttgggcaa
    11281 ataaaaagtt caacaataca aaagaagcta agtggttgta tgaaaatgca tataaatatg
    11341 gttttatact tagatatcca gaaggtaaag agaatataac aggatatatg tatgaatctt
    11401 ggcactatag atatgttgga actgaacata gcaaaaactt tgcaatgaat aatttgactt
    11461 tagaagaata tttacatata aattaagaga tggtttgtta aaattaggtt aaaataaaaa
    11521 ataacctaga ctattagctg tgtaaagtga tataatgtaa atacaattta agataactac
    11581 tcgaagggga gtagcttaca cgaaagtggt aattaatcgt caagacagta gaaatactcg
    11641 gttaattagc aagggattcc ttgatagcaa gaccttcaaa ataagcatta ggtatgtttg
    11701 ttttggggtc tttttttatt caaggaattt ttatttaaaa aaatttaaat ttcaagtata
    11761 cataagagcc cccaaacata aaataaattt attgaagagg agggcaacaa atgtcttaca
    11821 aaagtactta tcaagaagta attaaaaatt tagactcaag tttatctggt ttgagtaaag
    11881 aaagagcaga agagttgtta gaaaaaaatg gagctaatga attaaaagaa gcagataagg
    11941 ttcctactta taagcttttc ctagaaagtt ttaaagaccc attagtaata attctattga
    12001 tagctgcttt agtacaaata tttttaggtg aaacagtgga gtctataata atttttgcag
    12061 ttattattat aaactctgtt ctgggtgttg tacaaacaaa aaaagcagaa agttcattag
    12121 aaagtttgaa aaatttatct gcaccaaacg caaaggtaat aagagacaat aaaaaaatga
    12181 caattccagc aaaaaatcta gtagttggag atattgtatt cttagaagct ggcgattaca
    12241 taccagcaga tggaaggctt atagaagcac aatctctcaa ggttgtagaa gggatgttaa
    12301 ctggagaatc agaaccagtt ttaaagcata tagaaaaaat aagtgaagat gtaagtttag
    12361 gtgaccagaa aaatatggta tttagtggtg ctgtagttgt ttatggtaga ggttcttttg
    12421 tagtgactgg aacaggtatg aatactgaga tgggtaaaat agctgggctt ttagaaagtg
    12481 ctgaaaataa acttacacca ttgcaacaaa aaatagagga ttttagtaaa aaacttggtg
    12541 taggtattgt aatacttgct cttttaatat ttataattca agtagcaaga aattacttta
    12601 tggctggaaa tggagaaatg acacaaatta ttttagattc attcatgttc tctgtagcag
    12661 tagcagtagc agctatacca gaagctctat catcaatagt cactatagta ttggctgttg
    12721 gaacaaatag tatggcaaaa aaacaagcca taataagaaa attaccagca gtagaaactt
    12781 taggttcaac aagtgtaata tgtactgata aaacaggtac tcttacacaa aataaaatga
    12841 cagtagttga ttattatatg tatggaacta ataatgatga gttagataaa gaaaaagtaa
    12901 actattctta tcatgctaaa ttattgacta tggtttctac attgtgtaat gactctcata
    12961 taactagtga tggtaaagaa gttggtgacc caacagaagt ggctttaata aattattcaa
    13021 gtaaaaatga tttagattat gataaattaa gaagtaagta tatcagaata aatgaaatac
    13081 catttgactc agacagaaag cttatgtcta ctgtaaatag tatatatgga gattatataa
    13141 tgtttacaaa aggtgctcca gatattgtgt ttagtagatg taaatatgct ttgaaaaatg
    13201 gttcaaaagt agatataaca gatgagataa tagaagaata taaacataaa aatgaagagt
    13261 tctcaaatag agcacttaga gtattagctt ttgcaataaa agatgtccca gaagaagatt
    13321 ttataccttc aattgatgac gaacatgaca tgacattggt tggaataatg gcaatgatag
    13381 acccaccaag agaagaagta atggatgcag taaaagaggc aaagagtgct ggtataaaaa
    13441 ctgttatgat aactggtgac cataaaacaa cagcagcagc aattgcaaaa gagattggta
    13501 taatggaaga aggagacttg gctcttactg gaaaagagct tgatgctttg agtgaagaac
    13561 aactatatga aaaactagaa aatataagtg tatatgccag agtctcgccg gaaaataaaa
    13621 ttcgtatagt aaaagcatgg caacataaga ataatataac agcaatgact ggtgatggtg
    13681 ttaatgatgc acctgcatta aaacaagcag atataggaat cggtatggga agtggtactg
    13741 atgtagcaaa agatgcttct gctatggtat tggttgatga taactttgca agtatagtaa
    13801 atgctgttga agttggtaga acagtataca gtaatataat aaaatcaatt acttatctat
    13861 ttgcaggaaa cttaggagct atagtatcca tagtatttgc agtgtttgca ggttggtcaa
    13921 atccatttac agctttacaa cttttgttta taaacttagt aaatgactct ttaccagcaa
    13981 ttgcattagg gtttgaaaag tcagaaaaaa atataatgaa taaacctcct agacacccgg
    14041 atgaaagtat actagcagga ggaacattga aggcagttgc tataagaggt tgtataatag
    14101 gtgtagttac aataattgca caatatatag gattacaagt atctccagaa cttggagtag
    14161 caatggcatt ttcaacattg atattagcta gaataatgca aacattacct gcacgttcta
    14221 ataatcaaac agtaataaaa ttaggttttt tcagcaataa atacgttata ggagcagttt
    14281 tagtctgtct tgggctttac tctttaacac taataccagt gctaagagga atattctcta
    14341 taccaacaac gtttggattg taccagttgg ggatttgcct agggttagca ttaatttcat
    14401 caatattaat ggaacttaac aaattaataa atagaagtga agtaaaataa tcaaaaaatt
    14461 aaagggcata ttgattttaa caatatgcct tttataaata gtctaattta ttctatatta
    14521 tcgattattt ggaatgcttt tataacttca ggtaattctt tttcttcagg cattctaaat
    14581 cttataaaat ttttatcaag tccagtaaaa tcacttccag atataactct tatttttaat
    14641 ttagcaaact cttgttgtaa atctatgtcc ttgtttttat gttcaactgt cataattgat
    14701 acagtatcac tagtttcagc tatattaagg tttttccatg gaattaataa ttgatttttt
    14761 atatcttttg ttttttcttt taattcttct ataaattgaa catcatctaa taaatttgct
    14821 gcaatactac gagatatttc agacacttcg taaggagtag aaatcttttt aatagggcta
    14881 acaagttgtt ctggaagaac tgcatatcct gctcttagtc ctgctaaacc aaatcctttt
    14941 gaaaatgttt ttaaagcaat tacattatca taattattta gaagcttgac agctgaattt
    15001 tcttttggca tgtattcacc ataagcttca tctaccataa ctgctatatc atatttagct
    15061 gcttccctta ctatattttc aattgatgat aaaggtataa tttgtcctgt aggattgttt
    15121 ggattgtcta tgtaaattac tttatattct ggatttattt tttcaataaa ttctttttca
    15181 ttaaacttga aattatcctc tttctttaaa agaacataat cataagttgc accatgcatt
    15241 tttatatctg tctcatattc tgagaactga ggtgaatatc ctaaaacttt atctcccttt
    15301 tcaataaata gtctatttaa aaggtaaata acatgtatag aaccatctgc caaacaaatt
    15361 ctgtcagtat ctaatgctat aaaatctttc caataatcga ctatattatc ttttaaagca
    15421 attgaatgag gatatcctct tatcatatca aattttaaag cattaaaagc ttctacagct
    15481 ttgtttgagt aagacacggt atttatacct tcaccacagt ctatctcgaa tacatcttct
    15541 gaatgtccag attctattgc ataacttttt ttcaactgca tttcaatagc tttgttaatt
    15601 tttagtttca tactttatca ctccttattt agtcaataat aaatgttata aaagttgcta
    15661 gatttaaaat attatgaaaa tatataatag taaaaatgaa tataaatatt ataatagctc
    15721 actttattta atatgattat atatcatata ttgtaaaatt tctagcatta taagactata
    15781 aattgaaaat attcagaaaa atactacaag aaacgattta ttttgctctt aaaataagtt
    15841 aaatgaatgt tattaatttt gtaaattgaa atagtttaag tataaagtta tgatataatc
    15901 taataagggt aattctttag attatattaa ggaaatatag gtatacaata aaaagttatg
    15961 gagttggatt atgggatata aattaattgt aacagatatg gatggaactt tattaggcaa
    16021 taatcataaa gttacagatg aaaataaaac tgcactacaa aaagttataa aaagcggtat
    16081 taatgtaact ttagcaacag gaagagcctt cgattcagct aaatgcaatg tagattttct
    16141 taaagaggac atgccaataa tagcttgtaa tggttcatta ataagagagc aaaatggaaa
    16201 tataatatat tcaaataaga ttgatacaag aacatgttta aacattttag atgtattaga
    16261 taaatacgat atttactacc aatgtaatag tatagattct atgctttcga aaaaaataga
    16321 aggacgtgaa gatagattaa gtgtattttt aggctctgaa acagaagtaa ttgtaaaaga
    16381 tgatttgaga gaagaaatct ttaaaaaaga tatactaaaa tttgtaatca tagaagaaaa
    16441 aaatccatct attttggatg aaataagaaa agaattgaga aaagttcaag gaataaagat
    16501 aacctcttca tggccaaaca atattgaagt catgaatgaa ggtgttgata aaggtaatgc
    16561 agttaaaatt ttagctgaaa aaatgaatat agacagagaa gatataattg catttggaga
    16621 caattacaat gacatagaaa tgataaagtt tgctggtctt ggggtagcta tgggaaatgc
    16681 agaagaattg attaaacaag aagctgatta tgttacagat acaaatcaag acagtggagt
    16741 tgcaaaggca atttataaat tcctagagtt aaaagaatca cttagctctt aaaacagtat
    16801 ataataggaa aaaataggtt gactattttg tagatagtaa ttataataca aatagataaa
    16861 atataaaaat ttagattcag tgaaaaagag agtagatata gagaaaatct cagcgaattg
    16921 ggggatggtg tgagcctaat gataacactt tatattgaaa agaactttgg agaatactac
    16981 ttgaaattca gtatggtagt aggtaatctc gccttaagag aatagagtgg gtaaacattg
    17041 tttaccaact agagtggtaa cacggaatat ttcgtctctt ggtctttttg accaagagat
    17101 tttttagttt cattgagtta caagacttat aattaaaata caaaaaatta aaatatgagg
    17161 tgaaagttat gaaattatgg ggtggacgct ttagaaaagc ggaaaatcaa cttatggaag
    17221 aatttaataa atcttttggc tatgattgtg tactgtacaa aaaagatatc gaaggaagtg
    17281 tagcacatgt tcatatgcaa gtaaaatgtg gacttttaac agaagaagaa ggaaagtcta
    17341 taacagaagg tttaaaaggg atacttgaag atgtagaaaa tggaaagtta gtattggatg
    17401 gagagtatga agatatacat agttttactg agataaattt aattcaaaga attggagatg
    17461 ttggcaagaa gcttcataca gcacgtagta gaaatgacca agttgcagtt gatatgagat
    17521 tgtatgctaa agaaaaagca aatgatttgg tggatttaat atctgaattt aaagctacta
    17581 taaaagatgt tgctgacaaa aacccagtga tgatgccagg ctatactcat cttcaaaggg
    17641 cacaagtagt gacatttaag catcacttaa tggcatatta tagtatgttt gatagagatg
    17701 agaaaagaat aaaaaatgct atagaaatat tagatgaatc acctttaggc tgtggagctt
    17761 tagcaggaac tacgcatgat atagatagaa gtataacttg tgaacaatta ggatttaaaa
    17821 aagtagtaga taattttatg gatggagtaa gtgatagaga ttatttacta gagctaatga
    17881 gtgatttttc aattataatg atgcacttaa gtagattaag tgaagagttg atactttgga
    17941 gttctcaaga atttggattt gttgagatag atgatttata tacaactgga agtagtataa
    18001 tgcctcagaa gaaaaatcca gatggtgcag agcttataag aggtaagact ggtagagtat
    18061 atggaaactt atttggtctt tttacagtaa tgaaaggaat accattagca tataataaag
    18121 acatgcaaga agataaagaa ggttttttcg atagtgtaca tactcttgaa atgtgtatac
    18181 aaataatgga tagaatgata gccacactaa aggtaaatga ggataaaatg aaacaagcag
    18241 taaaaaatgg ttttttaaat gcaacagaag ttgcggatta tttggttaaa aataatgttg
    18301 cttttagaga tgcacatggt atagtaggaa gtatcgttat atattgtgaa gataataaaa
    18361 aagcaataga agatttaaca ttagaagaat tacaaaagtt ttcagatgct tttaaggaag
    18421 atatttatga ttttatcgac tatgagagta tattaaataa gggaataaaa aagaatttaa
    18481 aataatgtat ttatttttaa aagctgtaaa tttgaatgtt atcatttaaa tttacagctt
    18541 tttattgtgt aagat
//
LOCUS       DAYASV010000071        18485 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYASV010000071 DAYASV010000000
VERSION     DAYASV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18485
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.071"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..591)
                     /locus_tag="ACGI68_002356"
     CDS             complement(<1..591)
                     /locus_tag="ACGI68_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0138791.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="ACGI68_002357"
     CDS             complement(921..2135)
                     /gene="dpaL"
                     /locus_tag="ACGI68_002357"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFH0138792.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(3010..3297)
                     /locus_tag="ACGI68_002358"
     CDS             complement(3010..3297)
                     /locus_tag="ACGI68_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138793.1"
                     /translation="MQYAIELYYDKKTEKQLFDLSKKIADEKISTKYLEWKTRPHLTL
                     ACFNDVDEACCIDKLKGFAQNHKVIPAYIGSVGMFNDTKTVFVSPIMNSSM"
     gene            complement(3374..5179)
                     /locus_tag="ACGI68_002359"
     CDS             complement(3374..5179)
                     /locus_tag="ACGI68_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138794.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5575..5952)
                     /locus_tag="ACGI68_002360"
     CDS             complement(5575..5952)
                     /locus_tag="ACGI68_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0138795.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6857..7342
                     /locus_tag="ACGI68_002361"
     CDS             6857..7342
                     /locus_tag="ACGI68_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138796.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7542..8081)
                     /locus_tag="ACGI68_002362"
     CDS             complement(7542..8081)
                     /locus_tag="ACGI68_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0138797.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8074..8487)
                     /locus_tag="ACGI68_002363"
     CDS             complement(8074..8487)
                     /locus_tag="ACGI68_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0138798.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8523..9644)
                     /locus_tag="ACGI68_002364"
     CDS             complement(8523..9644)
                     /locus_tag="ACGI68_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0138799.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10232..11884
                     /locus_tag="ACGI68_002365"
     CDS             10232..11884
                     /locus_tag="ACGI68_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HFH0138800.1"
                     /translation="MNKKNLAILISGIMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            12183..12932
                     /locus_tag="ACGI68_002366"
     CDS             12183..12932
                     /locus_tag="ACGI68_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138801.1"
                     /translation="MNTILKVNNITKTYNKGQQPSLDKVSFNVSEGEFIGIMGASGSG
                     KTTLLNVLSTIDKPTSGDIFINNINLKSLNDTCAADFRKDNLGFIFQEYFLLDSLTIF
                     ENISVPLTLQKLNPDKIENMVKSLAERFGIASQLNKYPNELSGGQRQRASAVRAIVKH
                     PSILFADEPTGALDSNSATELLHSLQEANEELKTTILMVTHDAYVASFANRILIFKDG
                     HIIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            12945..14972
                     /locus_tag="ACGI68_002367"
     CDS             12945..14972
                     /locus_tag="ACGI68_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0138802.1"
                     /translation="MNIFYTAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKIM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENVLICSGAFIIGLVLGALAHKGIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAVSNGKFFYKTPLVKLIRYEKSAEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWVSIGFYQMGLITMIIIVIGTVLFINSFLPYIMQNSKKNKRTFYTDMK
                     IIAIPNFIYRIRSNSKTLIMLTLLSATTLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYTSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGTSKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLLKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYPLLVNGKETPVAVRETSLNNAISFANSVGTLIVNDSIYEQIAQNKLPMTRILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIAVG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYILTIKSCCKIVLKK"
     gene            15116..16699
                     /locus_tag="ACGI68_002368"
     CDS             15116..16699
                     /locus_tag="ACGI68_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0138803.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTETYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLALDSWYLLLSFVTTPIGTIIFKILSTVIVYVS
                     IKFIFLFLFQGSGYNFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHLKRVVFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRAIFLFISILVISLVVLFANGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPDVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNISDNGVANTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISVSDNIPHGV
                     CVQITIPMKGDGSYKYFVS"
     gene            16671..17291
                     /locus_tag="ACGI68_002369"
     CDS             16671..17291
                     /locus_tag="ACGI68_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0138804.1"
                     /translation="MVLINILLVDDHMLFAKSLSIALEEYKEIEQFYSTQDIHNLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGVTCFPSENTEVIIEDLTTMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIKMGYISPVY"
     gene            complement(17480..18406)
                     /locus_tag="ACGI68_002370"
     CDS             complement(17480..18406)
                     /locus_tag="ACGI68_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFH0138805.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         6335 a         2913 c         2224 g         7013 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaggtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttaatt tgttgacgtt ttattttgat atttcatttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt ttattttgaa tttttcattt gctttacttt
      781 tttatttgct ttactttttc atttatttag atttttcatt tgttttagtt tttattttat
      841 ttaaagtttt cacttattta agtttttatt ttccttaaat ttttcattta tttgttaaga
      901 ttttcttttc cttaagtctt ttatttactt tggcattcac cattccaaac tatatctcta
      961 tatttatcag ggtcagtatc tccttctgta cttattaata atactttaga gttctcatct
     1021 agtttcaaag cttctcttaa gtcttttaaa tcttcacttt caaggattga aactaatgct
     1081 ccagttgtaa ctgctccaga ttctccagag attactttct catctcctct tagagggttt
     1141 cctaatattc tcataccttt agcagatacc cagtctggtg ctgaaacgaa tgctgatgaa
     1201 taatttttta acacttcaaa acctatagtg tttggttctc cacaagcaag ccctgccatt
     1261 atagttggca tatctcctcc aacaaatctt ggttttccat ctccagcttc tgctgattta
     1321 tagtaacagt cagcctcatc agcctctact actactgtta ttgggcattc atcatatatt
     1381 gaagcaacat atccttgaac tgcaccagca agtgaaccaa ctcctgcttg aacaaatacg
     1441 tgtgttggtc tatctactcc atattctttt aattgttcta tagcttcttt agccatagtt
     1501 ccatatcctt gcattatcca tgctggtatt tcttcatatc cgtcccatgc tgtatcttga
     1561 accattactc catggtcatc agcttctgca taatcattag ctaatcttac tgcatcatca
     1621 tagtttaagt ctgttatact agcatctgca ccttctcctt tgattttatt taatctaaat
     1681 tcagaagaac cttttggcat taatacaaca gatttttgac ctaatttatt agctgtccaa
     1741 gcaactcctc taccatgatt tccatctgta gctgtaaaga aagttatttc tcctaatttt
     1801 tcttttattt cttttgaagt taatttatca taccctaact cagatatatc tgtatctaat
     1861 ctttgagcta agtatctacc catagagtaa gaaccaccta aaactttaaa tgcgtttaaa
     1921 ccaaatctat aagattcgtc tttaacatat attccgctta cgcctagtga ttttgctaag
     1981 ttatctaatt taactaaagg agttttttca tattgtggaa agctttcatg aaaagcttta
     2041 gcctttgcaa tctcttcatc ttttaaaaat tcaattcctt tttctttttc ctctgattta
     2101 ggtaagttat ttaatttcca ttttatttct ctcatttatt ttatattccc ctttcaaatt
     2161 tttcggtgtc attttaattt tttatctttg tgccttatag caaagcaatt accgtgccaa
     2221 tattatttat tctaaaaaca atttttttat gttggaatta catactttta taaaatttct
     2281 ttaaagttta taaattttga gtgtgaaaaa taagatttat aaaagaaaaa attctcatat
     2341 tgggaaattt ttatcaatac gagaattatc aattcgagaa atcgtttatt tatcttaatt
     2401 cttcctttat tatgctctta ttttttatat gtctattgtt ttgtcagata tattactact
     2461 tttattttct ataaatatat ggctataacc ttcttacgca taaactctat aaatttatat
     2521 actttatatt ctttattacc caaatattct ttattaccca aagcctccaa aatggcttaa
     2581 atcttttcat agcttcttac agaagtttac atgggcgtcc ccccaataag caaaagtgca
     2641 atatgtgtac cattgtgccc tacttgcgac tattgagctg tcgaaattcg caacttcgca
     2701 tttatctgat gaaatgcact atacaaaata ttcataacat ccacaacaga gttaaagtta
     2761 cctattcata ttcactgtaa aaatttcttc cacaggaaat gtaattttaa ccaatcctat
     2821 tgagacaaac tcaccactta tttttttgaa ctcacgcagt aatacatcac ttgcccgaaa
     2881 aaaagcctca ctttcatcat ctccagtaag tgctattgtg caatggggta cccaacgatt
     2941 ggggtggtac cattcccaac catttgaatc aaaatcttgt agtacctcat ataattctct
     3001 ctgaaactgc tacatactgc tattcattat aggtgataca aacaccgttt tagtatcatt
     3061 aaacatgcct actgaaccaa tgtaagcagg tattacctta tggttttgtg caaacccctt
     3121 tagcttgtct atacaacaag cctcatccac atcattgaaa caagccaaag taagatgagg
     3181 tcttgttttc cattctaaat acttcgtact aattttttca tcagcgattt ttttagataa
     3241 atcaaaaagt tgcttttccg tttttttatc ataatacagc tctatcgcat attgcataat
     3301 aacacctcca ccataaagta aaaataccta tctataaaaa cacacaaata cctatcattt
     3361 taatattata ttactactgt tcctcttcca attttctata tagtgtagcc aaacctatgc
     3421 ctaagtcttt tgctatcttc ttcttcgtct ttgtatctct tccatactta ttcaacaact
     3481 tgtttatgta agtcaactct aattctttaa ttgaaagtat cctttcttga ttctctacta
     3541 tccctccaac tatatcatct tcaaatatta tatttacatc cttattttta taaatatttc
     3601 cattaatatt ataatagttt aaaatatcta atggaagcat atctttatat attattccat
     3661 catctccaac caaattcatc atatattcaa ctgcattttc cagttccctt atatttcccg
     3721 gccaattata gttaatcaac atattcatta cattttcttc tattttattt aactgaatac
     3781 ctaattctaa agaatattta gaagcaaact tcatcattat tgcctcaata tcatcctttc
     3841 tttccctaag aggtggcaaa tttataggaa ttacattcaa tctatagtat aaatcatccc
     3901 taaacttacc ttcatttact aatttcaata agtttttatt tgtagcagca atcactctta
     3961 tatctaaatc tataagttta tttgacccaa ttctcgcaaa ttttctctct tgtaaaactc
     4021 taagtaactt tgcttgtaaa ggcattgaca aatctcctat ttcatcaaga aatataacac
     4081 cttcatttgc aagttcaaat ttacctactt tacctccact actagctcca ctaaatgcac
     4141 cttttatata gccaaatagc tcactttcaa ggagattttc tggaatagca gcacagttta
     4201 tcgcaataaa tggcttgttc catctgctac cttcagcatg tatagcccta gcaattagct
     4261 ctttacccgt accactttcc ccagttataa gtacagttga atttgacttt gctaatttct
     4321 tagttctctc ttttactttt aacattgctt ctgaatttcc aattatagac tctatatcac
     4381 tatttcccca gccactattc acttttccat gtcctttatg attgatatat gtcttgtcaa
     4441 atattattat tttgtcgtat tgatttatat atggatatac cggaattatt ttggcattta
     4501 tattataaat tttgttatca atatcaattt ctagtaattc atgtccattt gaggaagatt
     4561 caacagatac tatatttact atattttcta ttatattttt atctatatct aattttttta
     4621 gagctatatt gtttatataa gatattttgc tatttatgtc tagtattata acgccttttt
     4681 caacattttc aatgatttct atcatcatat ccatttttct actgctattt tttgacgctt
     4741 catgttcttc tattttcata gaaattaaat cgcatatatg attgacaaat tttaaataat
     4801 tatctatttt tgcacttatt tctacttttt tagttttatc tgtactaata gcaccaatta
     4861 ctcctatagt tcgattatta tactttattg gaactgctat tattacttta tttaggcatt
     4921 tcatgtaatg ggaacattct atacaaagct gattatcaca tatatccaat acgactaatt
     4981 cctgacctgt ctgtataaca ttgtcataaa taaagccctc agtaacaacg ctttcattta
     5041 tctttgattt atacagacct gtaccactaa ttcttattaa tctatcgtca actatttcaa
     5101 tatctacatc taacacacta gctactgttt cagtgtattt tatcatttct tgttgaaatt
     5161 cttttaattt acttaacata gtttccccca ttagaattta atttaccaaa ttatcagttt
     5221 agctatattt taacatatag tcactcttta aaatatatat aaaacattac ttatttatat
     5281 atttgcgtta aaacagacaa tattaattta taaaaagttt gttttatata gtaaccttaa
     5341 ttataatatg cagacaataa aataaatcac aatacaataa taaaaaaaag ctttcctcat
     5401 ataacaaata tatttttgac ataaaaatat acattatatg agaaaagctc aatagttaaa
     5461 tatttagata gatttttata tagacttttt taatataaac ttttttaatt gtagactttt
     5521 tttattagtt tttaattttt attagttttg ttattggttt tattgttata atacttataa
     5581 tactgccatt gcttcaattt ctaaaactgc atcttttggt aattgagcaa cttgtacaca
     5641 tgaccttgct ggtggatttt ctttaaaata tgtagcatat acttcattaa tatcattaaa
     5701 atcctttaaa tcttttacga atactgtagt cttaaatatt ttatccatac ttgtaccagc
     5761 ttgttctaat attgctttta cattatctaa agaagctttt gttgcttctt ttgggtcaga
     5821 tactaactcg ccagtcttca tatctattgg aagttgacct gatgtagaaa ctatgtttcc
     5881 aacttgaatt gcttgagaat atggtccaat tgctcctgga gctttctctg tagctattac
     5941 tacttttgac atgacttcac ctcctattat taagttatat ttacaggaaa ataaaatttc
     6001 ctaaacacca atattaatac gaacctttag attaaacttc aaatctaaaa cttcaaataa
     6061 tcaatactta gatgattatg tttatttgtg tcttgttaac ttttattaaa gcaattaacg
     6121 tgccaaactt tatttatata taaaattttg tgaagtcatt caaaatagtt acttctgaat
     6181 tttttcgtaa aaaaacgctc taaaattact tttttatgat gtcaattttt ttctcatatc
     6241 tagaaattta ccaacatgag aattcttgtt ttgagaattt acagattaaa gttatttttt
     6301 aaccttaaat tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt
     6361 gcataatcaa gttattacat atgaaagaac tatagttgac aatcccacta ttataaataa
     6421 taaaaccttt acccatacct tatttaccct agttatcata gagcctattg aaaatataac
     6481 agctaaatat aataataacc aaaaaattat caatatctac cactgttgct gaaaatccaa
     6541 aaaactacct tttgatagta ttacttaaca atctaattaa tctcataata aatttacaac
     6601 ttataaataa attgttatat tggccgtatc tcaacaactt tctcctttcc tagttttaaa
     6661 tcttttatag aattgtacca cgtctaagta aattttcata ctattaagtc ttatttctaa
     6721 taaatcatta gctctcattc tatgcccaat aatgtaagat tgcagtaatt ttattgattt
     6781 taatagtttt atttaatata cttatattaa ttattttcat atgaaattat tatttatata
     6841 gggggatttt tttaatatga gcaaaagtga attaacagca gaaacaacag aagaaatgtt
     6901 agaagtacta agtggtaaag attatgatat tgcatgtcat ttacatgaac ttggtaaatc
     6961 attagattgt aaaattgaac caaaaacagg tgctcgttct tacaaaatag tatattcaac
     7021 taagaaacca aaacgtagct tatttactat tgaatgtaat gaaaaaaaat ggagagttaa
     7081 agcaaatctt tttcatctaa atacatataa agatgctgtg gaggaatgct ctaaaactat
     7141 taaagatagt ataactaaaa ctcgtacttg taagaaatgt aattcaaagt gtattggagg
     7201 ttcttcgttt gaattagatg gaaagtctta cctgacttgc ataggaagtg gtcattattt
     7261 tgcaaatatg gaagaaatgg attggaaaaa cctagaaaaa ttaattacta aagaaaataa
     7321 tattatgcag gaatctgtat agttttttaa gggtaattat taaaatatat gagtaacaac
     7381 agatacttaa ataggcataa aaaaataaag ggtataataa ggatatagaa taaaaataaa
     7441 tgatatagca caaagaggct gtctcaaaat acagataagc ctctttttat tgttgaaatg
     7501 ataagaatat attcaatttt atacaatttt atacaaacat actacgccaa cttacccatt
     7561 tcatatgcct gtttcattgc cttacttccc ttaatatcac ctacattcca agcacctaca
     7621 ccatatacta tacctttttc cttagaacca tttagacatg atgtaaatcc tctaaatcct
     7681 tctattgttc tttccatatc agccacattt tcatctgctg ctgtaacgat aaaatagaaa
     7741 tctttatcac aaatttcagt atatctagag caagttctat caatcatagt tttcatttgc
     7801 gcattcatag tataaaaata aactggagtc gctaaaacaa tcacatctgc ctcaatcatt
     7861 ttttcaagaa tttctcccat gtcatctgtc tgtgaacaat ttgcttcttt ttcataacaa
     7921 gcaccacatc cagtacaata gttaatatct ttatctttta aaaatatctt ctccacttga
     7981 tttttagatt ctaatacccc ttgtataaac tggtcacaca ataaatcaga atttccacct
     8041 tttctaggac tagctgatac tattaataca tttttactca taagatactg cctccatttt
     8101 cttatttcta ctgctcttta atactttttc tttctcaata ataccttctt catatctatc
     8161 aatcttaaaa tttaatcgtt ccaaagtttt ttgcatttct tctattcttt ttgctagttg
     8221 gtttctctgc tctattaaaa gttcttttct agctttgata gtactgctcc cttgttgaaa
     8281 catagctaca tactcaatta atgtctcaat agaaagacct gcacctctca tacactttat
     8341 gaaattaacc cactcacaat cttcttctgt aaaagaccta attccacttt tatttctatt
     8401 tacaggtggt attaaaccaa ttcgttcata atatcgtaaa gtatccgctg aaagctcgta
     8461 ttttttacta acttctgaaa tcgtcataca tatcttctcc tcttgatatt gtatagattg
     8521 aattatttct caaaggtttc tgctacattc tttaaagcat ctataatttt aatgtgttga
     8581 ggacagcttt cttcacattg tttacattca atacaatctg aggcctttcc ataagtttta
     8641 gtataattat tatagtagac catctgtgtt gaaaatccag tattaagagc ttgtttttct
     8701 gcattgtaca acgcaaaata gtttggaata gcgatatttt ttggacagcc ttctacacaa
     8761 tactgacagg cagtacaagg aactaaaatt gattcgttga taatctctac tgcttcatct
     8821 ataatattat attcctcttg tacgaatggt ttgaaatttt gcatatatcc tgtattgtct
     8881 aataattgtt ccatatttga catcccactt aagaccatca taacattatc tttacttgct
     8941 gcaaaacgta ttgcccaaga tgaaattgac atatctggat tgtagtcatt caataatttc
     9001 tctgcttttt ctggtatctt tgcaagtgta cctcctttta ctggctccat aacaattaca
     9061 ggcttattat gcttctctgc cacctcgtag cattttctag attgtatgct ttcattatcc
     9121 caatctaaat aattaatctg taattgcacg aagtctactt ctggatgctc actcaagact
     9181 ttatccaata attctgcact atcatgaaaa gaaaaaccaa tattctttat ttttccttct
     9241 tttttctttt cttcaataaa cttaaagcta tcaaattttt tagctacttc ataatgtgac
     9301 acgcctatat tgtgtaaaag atagtagtca aaatagtcta caccacattt ttctaattgc
     9361 tcattaaaaa tggtttcttg ctcttctttt ttctttaaag ccattaatgg aagttttgta
     9421 gctatagtaa aactctctct tttgtgtctt tttactagag attctctaag tgctatctca
     9481 ctttttccca tgtgatacac ataggctgta tcaaaatatg taaatcccct ttctaaaaaa
     9541 gtgtctacca ttttatttaa atgttccata tcaatagttg taacatcatt ttcatctttt
     9601 attggtaaac gcattaatcc aaatcctaat ttttttgcct ccattgtttt acttccccct
     9661 ttaaaacttg tttgtttttt ctctctactt taactattct tacaagtata taattcttgt
     9721 ataattatac tttactactt ggagttaact tcaagtcaat agattttttt atttttaaga
     9781 caaatattat aaaaatacag ttctttaata ttaatccatt acatgtttct ttcaccctcc
     9841 aattctcaaa cttcacaaac tccaacatgt aaagaaatat atactttaag aaattcagtc
     9901 cattaatatt actacaaata ctttttagat atgtttaaaa agaaatttca cctaaaagat
     9961 ttagaaatag gtcttgtgtc tattttaaaa tatataaaac agtggtaaag acttatttat
    10021 aagataaaaa atgaatagtg tatacccttt ttaaaaagtt ttttcctttt tatatttgtg
    10081 atacaatgtt aaatataaaa aggaaaattt tgtattaaaa aaatcttatt tgttgatttt
    10141 gtaacttcaa actcagcatc aatttgattt taagattata ttttattaag tatgctaaat
    10201 aacaaatttt taaagattgt gaggtaaaaa tatgaataag aaaaatttag ctatattaat
    10261 atcaggtatt atgttttttt ctagttcttc agcaattttt gcagacaata ctacaaagca
    10321 agaaagatta ataggtaaaa caagatatga aacagcagta gaagtaagta aattgggatg
    10381 ggttcaatca aaaacagcca ttatagttaa tggtaattct attcaaagtg cgctttgtgc
    10441 aaatccattt gctaagctaa agaatgcacc tatactttta gtaaacaaca acagtattga
    10501 aaattcaact aaggctgagc ttaaaaggct tggtgtagat aatgtgtaca ttgtagatag
    10561 tggaaatagc atttcttcta aagtagaaaa tgaaataaaa tcacttaaca taaaaataaa
    10621 taaaatagta ggaaacaata tatatgaaat gtctacaaat gtacttaaag aaatagataa
    10681 gataaaaaag gtagaaaatg ttgcagttgt aaatggacta aagggactat ctgatgcagt
    10741 tagtatagct tctccagcag caatacataa tatgcctata atattagtat cagatgaaaa
    10801 cagcaatgat agtagtataa atttcttaaa caataggtct ataaaaaaat cttacatagt
    10861 tggaggaaat aagattttat cagacaatac agtaaagaaa tatccaaaca gagaaagaat
    10921 cagtggttca gatagaaaca atactaatgc aaaaattatt gataaatttt ataaagataa
    10981 aaaaataaat aatgtatttg tagctaaaga tggtattaac caagaaaatg cattagtaga
    11041 ttcattatca ataggagtac tagctgctaa agaaaattct cctataatga tatctaatgg
    11101 tagccttgta aatagtcaaa aagaatttat taaaaataaa gatatagatg aaataacaca
    11161 agtaggagga ggatataatc aaaaagcatt tgaagatatt ttaaaactaa aaaatgtgaa
    11221 cataagaaat aaaactaaaa aaatatcttt aatacaggct aaagaaatat taaaacaagc
    11281 tcttccagga agcaatattt tagtttgtta ttatgatgaa gatgataatg aatatgactg
    11341 caaagttata aaggataata tcctatatga gatagaaatt gatgcctact ctggtaaaat
    11401 aatagaaatt gaaaaagatg atgacttaaa tgataataat ttaggtacac aaaatatatc
    11461 attggataaa gctaagtcta tagcattaga tgctgttcct aatggtaatt taatctattg
    11521 taaatatgat gctgaagata acgaatatga ggctaaagta ataaaaaata agaaaacata
    11581 tgaattagag ataagtgcat ttgatggaag aattgtagat attgacatgg acaatgattc
    11641 tgatgatgac tttgatgata atgataagaa taactctaat actaatgaca acagttctat
    11701 tattagtgct gaaaaagcta aatcaataat gctagataaa gtgcctggtg ggaaaatagt
    11761 aaaatttaag tatgatgaag atgacaaaga atatgaaggt gaaataataa aaggaaaaac
    11821 tgaatatgag ataacaatta atgcaattac tggtaaaata ttagaatttg aaatagatga
    11881 ttaataaaaa atatatattc tattaattat tacaagcaat aaagccataa atattgaaaa
    11941 tttcaatatt tatggcttta ttttattttt tttaatttaa atccacatta tgccattata
    12001 tatacctatg cactctttta aattaaaatc ttgtccatca tcccaaacct aataaagtta
    12061 gattttaaac aatatattgt gatataagta atttgtgtag aattattgcg tgatttactc
    12121 atgaagaaac aataaggtta atatataatt tgactatatt aagacgaaag gagacatata
    12181 caatgaatac aatattaaaa gtaaataaca taactaaaac ttataataaa gggcaacagc
    12241 cgagtttaga taaggtatcc tttaatgtat cagaaggtga atttataggt ataatgggag
    12301 cttctggctc tgggaaaaca actctactta atgtattatc tacaattgat aaaccaacaa
    12361 gtggagatat tttcataaat aatataaatt tgaaatcgct taatgacact tgtgctgctg
    12421 attttcgtaa ggataattta ggctttatct ttcaagagta ttttttacta gatagtctta
    12481 caattttcga gaatatttca gtacctctta cacttcagaa actcaatcca gataaaatag
    12541 aaaacatggt aaagagtcta gctgaacgat ttggcatagc ttctcaatta aataaatacc
    12601 caaatgaatt atctggtggg caaagacaga gagcttcagc agttagagca atagtaaaac
    12661 atcctagtat tctctttgca gatgaaccaa caggagcttt ggattctaat tcagctactg
    12721 aactactcca ctcactacag gaagcaaatg aggagttgaa aacaacaatt ttaatggtaa
    12781 cacatgatgc ctatgtagca agttttgcaa atagaatttt gatttttaaa gatggtcata
    12841 tcattaaaga attaaataaa caacagaata atagacatac attttatgag ttgattattc
    12901 aagaaattgc aaaattggat actaaaagat aggaggataa tttaatgaac attttttata
    12961 cggcaataaa gaatcttaaa aaaaactttt cattctattc tctatatcta ctttcagtct
    13021 cttttgttat tactgtattt ttcgcattta cttcattttc tgttaataag ataatgctag
    13081 aaaaaatttc aactgatggt agagttgaat ctatgtgtaa tgttatttcg atatttttga
    13141 tggcatttgt catcttctat atggcatact caaatcgatt cttcttgaga cgtcgtacaa
    13201 aagaattagg tatatatgct ttacttggct atcgtaagtt tacaatatta tcgctactta
    13261 cctttgaaaa tgtattaatt tgtagtggag cttttattat tggattggta ttaggagcat
    13321 tggcacataa gggtattgtc tttggaatta cagcactact taatttatca attgataatt
    13381 cacagatacc attttttaat ataaatgcaa taaataaaac aattatcttt atagcattag
    13441 ttgttttagt cttagctgta tcaaatggta aattttttta taagactcca cttgtaaaat
    13501 tgataagata tgaaaaaagt gcagaaaaaa aattaaagtt cagaaaaatt ccagccttta
    13561 tggggtttat tatgatttta agcgggtatt gtttatcact agatattctt cgtggagcta
    13621 aatcagtatg ggtaagtatt ggattttatc aaatgggctt gataactatg attatcattg
    13681 taattggtac agttctcttt atcaattcct ttcttccata tattatgcaa aatagtaaaa
    13741 aaaataaacg tactttctac acagatatga agataatagc tattccaaac tttatctatc
    13801 gtatacgttc aaactctaaa actctaatta tgcttacatt actctctgct actactttga
    13861 cagtttcaag tgttatggca ctgtccttat actaccctat tgcatctgtt tcgcgtattg
    13921 caccttcaga gatagaattt agagtagaaa aaaataaaca aatagaagat gtaaaacaaa
    13981 ttgtcagtca atatacgtct aatacagatg atattatttt tacacagaca aatatttata
    14041 aggtaaaatc ttcatcaaaa gaccttccat ttgaatatga aattggtaca tctaagaata
    14101 atttatccaa tagtagttct tctaaagaag ttatttcaag agaatcagga tttgaatgta
    14161 tatctttttc tgattattat actttattaa aggcacaaaa tcgaacaaag ctactaaaac
    14221 aacttacccc tttaagggat aatgaatgta ttttaatgaa atatcaacca aataatgatg
    14281 gatatagtga aaatggaacg atttatccat tacttgttaa tggtaaagaa acacctgtag
    14341 ctgtaaggga aacatcatta aacaatgcta tttcttttgc caatagcgtt ggaacactta
    14401 tcgttaatga tagtatatat gagcaaatag cacaaaataa attaccaatg acaagaattt
    14461 taagtattaa tggtaaatca attaaaaata atgaaaattt atatagtaat atttcagatt
    14521 atttagatgg aagcccttat ttacaaggat attctcacag gataaatgat ttattatcaa
    14581 ttaatagttc tacatttctg ttaatcggat ttcttgtggt attattcttt attgctgttg
    14641 gaagtatttt atactttaac aatatatcag caatttcaga ttcaacatct gattataaaa
    14701 ttttacagaa aatgggttat actaatatac aaattaaaaa aattattaaa aaacaagtat
    14761 tgccattttt taatattccg tttttacttg gtatagtgga ctgtatattt gctaccttgg
    14821 tttataagac tgctttgatg cagaatttac ttggaaattc actttcacaa tacatacctg
    14881 tgattattgc aattttctta acttctgtag tatatttgat atattacata cttactatta
    14941 aatcctgttg taaaattgta ttaaaaaaat aatatttgcc aaatcgtcaa gcttaaaggt
    15001 gtgacgattt ggtatacttc atttataagt atttgatata atttaaaact ataaattctt
    15061 aagtttgatt tagtcataaa attaatataa ttaactcata aggaggtgta taagattgac
    15121 aaattttaat ttaaaaatac tatttttagg aaactacatt ctatcacttc tcttcatggg
    15181 aatatctaca agcactgaaa cttatatgaa tattcaaagc attattccct taataatttc
    15241 aacattatta ctcatagaca tcacatatat aacctcttgt aatgtccgag aaaataacat
    15301 tatttcattg ttttgtggat tactagcact agacagttgg tatttactct tatcatttgt
    15361 aaccacacca ataggcacta ttatttttaa aatattaagc acagttatag tgtatgtctc
    15421 tattaaattt atatttttat ttttgtttca aggtagtgga tacaatttcc gtaagataac
    15481 agacatttta ttgcttatca cctgcattgg tgcaattgta ggagcattta tttctaacag
    15541 aacgtatgct tgtatgtacg gaatacagtt tgttgttagt ataataagct ttttctatat
    15601 tattttttat catttgaaaa gggttgtttt tgtcataaaa agcgaaaaaa aacctatatc
    15661 gatttcagta tttataacaa ttataggatt ccttacttat tattttttta cactaagaat
    15721 atcaaatcat attggtaatt ttggaatcta tctaactgta ctggtatttt ttatgagtat
    15781 tcatggtatt gcacttaaag aaaatgatag ttttcctatt tcaacagttt ttaacataaa
    15841 acaaagggct atatttcttt ttataagtat tttagtaatt agcttggtcg tactatttgc
    15901 caatgggaat ttttctataa tattaatagc tataaatgtt ttatttatgc tcattttcct
    15961 ctgtaacatt attttggagt ttaaccttag agatgaaaaa aataaagtag ttaaaaatag
    16021 caagtatact tttgcattag aaaaattaca acaagaagaa caactaaaaa cagagttttc
    16081 taatttttta catgatgaca tattacagaa cctgctttca ataaaaaaca tgatgagtaa
    16141 gtcatatcgc ccagatgttc aagatattat atatgaaaca cttaacaatc taaatacatg
    16201 tataaggaag caaatgcaag actatcatcc tgttatttta aaaaatttaa cagtaaaaga
    16261 aaattatcag aatttaattg aaagtgtttc ttcatccttt ccacaaaaaa atatttgtgt
    16321 ttcatttaag tgttctgata ctttattctt agttgaacca tatgatatat tgatatatcg
    16381 actgattaaa gaattattga caaatgtgta taaacattca aatggtagcc atgcatggat
    16441 agctttagca ctagaaaaaa atataataaa attaaatatt agtgataatg gtgtggcaaa
    16501 tacatctttt aaaacgcctt tagatataac taaaattact tttgatacaa taaaacacaa
    16561 agggattata tcaattaaag aacaagtaga aaatctagga ggaacgataa gtgtttctga
    16621 taatatacct catggagttt gtgtacagat tacaatccct atgaaaggag atggttctta
    16681 taaatatttt gttagttgat gaccatatgc tttttgctaa aagcttgtca attgcactag
    16741 aagaatacaa agaaatcgaa cagttttatt ccacacagga catccataat ctttcagaaa
    16801 tgattcaaga taaaaaaata gacattgtct taatggatat aaaccttaga ggcttaagtg
    16861 aatgtgatgg cttaaaagta gcttataatt tattagagac taattcaact attaaaataa
    16921 taatcttgac tggatacgac ctcccagttt ataaacatga ggctaaaaaa ataggtatct
    16981 atggattcct caataagaat attagccctg atagtttaat tgattcatta ttaaaagtct
    17041 atcaaggggt aacttgtttt ccatctgaaa atactgaagt aattattgaa gatttaacta
    17101 ctatggagaa aagaatttta cagcttatct gtagtggtaa aaaaagaaaa ataattgctg
    17161 atgaattgta tataagtgaa agaacattat caaatcattt acaacacata tatgacaagc
    17221 tagatgtttc atcatcagtt gaggctatta caaaagctat taaaatgggt tatatttctc
    17281 cagtatatta aaaaaagtga gtgtctcaaa ataaatcttc taactcataa agcactctcc
    17341 tttgtattaa atcaaatata ttttcatcat ttaaacaatt aagaagaggc ttctctctat
    17401 tttgagacaa ctccatttta atacactatt tatatttata tataaacata ttttaaacaa
    17461 ctaaaaagca actaatatct caatacatat cttcataaca catatcacca taattacaag
    17521 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    17581 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    17641 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    17701 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    17761 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    17821 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    17881 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    17941 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    18001 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    18061 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    18121 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    18181 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    18241 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    18301 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    18361 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    18421 aatttaaata gataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    18481 atttt
//
LOCUS       DAYASV010000072        18389 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYASV010000072 DAYASV010000000
VERSION     DAYASV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.072"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(213..893)
                     /locus_tag="ACGI68_002371"
     CDS             complement(213..893)
                     /locus_tag="ACGI68_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138806.1"
                     /translation="MQTKILIIDGDKDNCKKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(943..2958)
                     /locus_tag="ACGI68_002372"
     CDS             complement(943..2958)
                     /locus_tag="ACGI68_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0138807.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLYFL"
     gene            complement(3030..3707)
                     /locus_tag="ACGI68_002373"
     CDS             complement(3030..3707)
                     /locus_tag="ACGI68_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0138808.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4287..4370)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4603..5445)
                     /locus_tag="ACGI68_002374"
     CDS             complement(4603..5445)
                     /locus_tag="ACGI68_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0138809.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5697..7481)
                     /gene="pepF"
                     /locus_tag="ACGI68_002375"
     CDS             complement(5697..7481)
                     /gene="pepF"
                     /locus_tag="ACGI68_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFH0138810.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYAKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7547..8212)
                     /locus_tag="ACGI68_002376"
     CDS             complement(7547..8212)
                     /locus_tag="ACGI68_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFH0138811.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8363..8524)
                     /locus_tag="ACGI68_002377"
     CDS             complement(8363..8524)
                     /locus_tag="ACGI68_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HFH0138812.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8602..9111)
                     /locus_tag="ACGI68_002378"
     CDS             complement(8602..9111)
                     /locus_tag="ACGI68_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFH0138813.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9317..9535
                     /locus_tag="ACGI68_002379"
     CDS             9317..9535
                     /locus_tag="ACGI68_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0138814.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9553..11499
                     /gene="feoB"
                     /locus_tag="ACGI68_002380"
     CDS             9553..11499
                     /gene="feoB"
                     /locus_tag="ACGI68_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0138815.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIIEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11678..12733)
                     /locus_tag="ACGI68_002381"
     CDS             complement(11678..12733)
                     /locus_tag="ACGI68_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0138816.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12781..13581)
                     /locus_tag="ACGI68_002382"
     CDS             complement(12781..13581)
                     /locus_tag="ACGI68_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0138817.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13574..14365)
                     /locus_tag="ACGI68_002383"
     CDS             complement(13574..14365)
                     /locus_tag="ACGI68_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0138818.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14405..14827)
                     /locus_tag="ACGI68_002384"
     CDS             complement(14405..14827)
                     /locus_tag="ACGI68_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138819.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14843..15322)
                     /locus_tag="ACGI68_002385"
     CDS             complement(14843..15322)
                     /locus_tag="ACGI68_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0138820.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15454..18210)
                     /locus_tag="ACGI68_002386"
     CDS             complement(15454..18210)
                     /locus_tag="ACGI68_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138821.1"
                     /translation="MRDEILSFVNECVLFNNFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDIIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6013 a         3148 c         2022 g         7206 t
ORIGIN      
        1 tacaatttat ctatcttttc aaatataatc tataaaataa tagttccatt acgcattttt
       61 ttatataata aaactgactg taatttaact ggcaaagtct agaagaatag gctttatcaa
      121 ttaaattaca gtcagctatt ttataataat ttagtttaga tattgttagt atttcacatt
      181 tataacaatt attaatgata ttaatatatt tattattctt tattaaaata atatcctgct
      241 cctcttattg ttataatttt ctgaggattt ttataatctt tctctatttt ctttcttaat
      301 ccattcactg caactaccac actatagtta tcctctaagt attcattcat ccatacatgt
      361 tcatatatct ctttagtagt caaagtcttt tcaggattct ctataaaaaa gtttaacaac
      421 ttattttctg ttggagtcaa ttttattatt ttatcttcta actttattac tccactagtt
      481 ctattatatt cctctaattt tgtattattt tcattattat ttacatatga gttcattctt
      541 cttaaaatag cttttacttt tgcgtataat atttctaaat ttagaggctt tattaagtaa
      601 tcgtctccac cagaatttaa agcatttaaa atgctttggt cttcatttat ataagtcata
      661 taaacaattg gacaagtagt tacatttcta atttttttac aaagagtcca tccatcacca
      721 tcacttagaa ttatttctaa aaatattaaa tcatatttat tgctaaatat ttttccaata
      781 gcttcttcac aattatatgc taaatctata cttattcctt tttcctccag aaatcctttt
      841 agcttcttgc aattatcctt gtctccatct attatcaata tcttagtttg cattataatc
      901 actcccaaat atcattattc ttttatatta aataagctat tattataaaa aatatagact
      961 taattttatt gtaaaaacag ttccttttcc tagttctgaa tctacaaaga tttctccacc
     1021 atgctctctt ataatggatt tgacaatata tagccctaac ccttgccctt ttggctcatt
     1081 attgtctctt tctgtaaaga atctgtcaaa tacctttggt atattttctg gaggtatacc
     1141 ttttccatta tcagaaattt taataattgc aaatccatct tctttatcca ttgataaaat
     1201 tatctttcca tcaaatggag taaaggtcaa agcattataa attaaattct caaatgctct
     1261 tgtaagcatc tctttattta tctttgcata tatttttttc tccaatacgt aagtttcaaa
     1321 gtttacacca catgcttgtg tatctggcaa attattttca tatatctctt taataaaatt
     1381 attcagacat attttttcac taataccaat gcttttatct tgtgatgtaa attcttgcaa
     1441 actacgtact ctattttgta cttcatttga cttttgattt atagcataaa gataggttct
     1501 tgtctcttca tctacatgaa tattaccaac ctttatcaaa tctataaatg cttgtatagc
     1561 cgaaacaggt gcctttaaat catgtgctac atctgccaag aatcttttac gttcatttaa
     1621 caatgttgta agttcattag ttctcacttc aacttcatgt tcaagattcc ttgtaagttt
     1681 ttcattctct tttattatta ttctattcct gtttatcatc ataacagaaa ataaaataac
     1741 aattacaaac ccacaatatt ctgtttgcca ccctgtaaat ataggctcaa atctattact
     1801 cgtaactgca tcaaataata tgctaattcc aaaaacagtg tttactgaaa ctaaaatata
     1861 agaacttata tgctcagagc atgtcttttt atatattgaa accagcgaag acattaatat
     1921 atatataaat acaatatatt tatatatgtc tattaacttc ccatatatgt tgacaaagta
     1981 catatcatat ggaagaagaa taattggtat cactatagat atgatactca ttgtaattcc
     2041 taatggtaaa attaaaaatc tgtacacttt attttcttct attttggata tacttccatt
     2101 tatacaaata atacataaaa ctaccataaa atatgacaaa tcttcaaccg catatgtaaa
     2161 gcttaccaaa ggcagtccta acaaatgtac aaagggatag gatacatgga tggcaaagaa
     2221 aacacacata catccaaaat ataagtaaat tttacttttg tttgaaagaa accatacaga
     2281 caatgaaaat atagctattg caaaagttgt gaagcaaatt atactataaa aaagtaatcg
     2341 ataaaacatc atattactta tgtcctttgt ttctcctaat gcaggcggat aatacaaacc
     2401 actataataa tgtgaaaaat tcgatacatt gattataatt tctgtatttt tctttgcaat
     2461 aaaacttact actgaatttt taattttggg tctatatttt tctgttccta catctcctaa
     2521 tttagaaacc atttctccat ttatccatac ttcacttgcg gaaaaaatct caggaagctc
     2581 taaacttagt agcttatcct tgccttcatt ttttattctc atacgataag tagcattccc
     2641 atatggtgac tgagtcccct ttctcataga aaaatctgga tattgaccta taaaaacata
     2701 ttctggtttt atatcttgct ctgtaaaatc attcggttta aatattttat gattataaaa
     2761 ctcccagtca tctatcaaaa agacaagttt acctttagtg aaagtatcat tatctatagt
     2821 taaaattcca ttacttgctc taatagactt atatgtatat ttattatcat atttatacag
     2881 aagatttaat cctgttattg ccaacagaga gactatcaat acaatgagag ttccttttag
     2941 tatattatac tttttcattg tatcaaccac atctctttca tagtagtatt aaataaaaca
     3001 agaactattt attaattaca gtatctcact tatggtacaa acttatatcc ttttccccat
     3061 acggtttgaa tgtattgatg ctccctgctt aaagaattaa ttttttttct catctgtgca
     3121 atatgaacct gcactgtatg ttgatctcca atatcaggca ttttccaaac ttggttatat
     3181 atctctatct tcgaaaatac ttgttctttg ttttcagcta gaaacaataa tatttcaaat
     3241 tccatagtag ttaaatctat actttctcca tcaaaagtta cttttcttga atttacattt
     3301 attactaacg ctgaaaactt taacacatta ggaactgatg taatattttt gtactgcctc
     3361 atacgagcat atattctaag ttcaagttct tttaaactat aaggttttgt tatataatca
     3421 tcccccccag ctaaaaatcc ccgaactttt tcttcttcat gctcataatt agataaaaat
     3481 attataggta tgttaacctt ttcttttata tcattgcaca ttttatatcc atctatatct
     3541 ggcaatgaaa tatccaaaat tatacagtct aaaactatac tctctactat atccatagca
     3601 tctttagcag tctctgatgt atatacatta aaacctatat tagaaaaata aatttgattt
     3661 atgtttaaaa tatcaacatc atcatctaca accagtaaat tatacattac accactccat
     3721 tcaaaggtat tttaattata attccaagtt ttatagttat tacaaattat aatgttttat
     3781 tttcatatat aactggtatt attattttat aatatcttta caaactagac tataatactt
     3841 aagtattttt gaataattct tgaatattgt attaaacatt aagattcatt attaaatact
     3901 aattaaagtc tccgattgtt ggagaatgga gcttaaagtc attttcacgt ataaatttat
     3961 tatttttcta gaaacttgtc tattggctag tttgagtatt gatttatatg atttatatgg
     4021 ttataatata aaataaattg tattattttg gtaaatatat tgttacaaaa ggtttatatt
     4081 ctgcatctca atgtttataa aaactttatt ttcttattat aatactatta ttcttttaaa
     4141 aaaatttccc ttttccaaca aatggctact ttaaaagcca aaattttacc tatcaataaa
     4201 aatcttaacg actaatactc ttattttcaa attttgcaac atatttgtta attttaaaac
     4261 aaaaaaagac tatgcaatat aaaaattata ttcatagccg gttgcaccac taactccata
     4321 tatctctaga tgcccaataa taagatatac ttctcagttt ctaaaatatt tttattaaaa
     4381 atcttttata tattttcata aatttctttt ttattctaac attgtaatat tattttgtca
     4441 atatttccat attcttagag ataagatact aaaataccta ttttatctaa agatatttat
     4501 atttctacat tcaataatag acaataccct gcaagttaaa gcatcatttt tatgagctta
     4561 tctcacaggg tattatatgt ttactttata ttataactct tgttattgat taactttaat
     4621 aaatcctttt tctgaaacaa gtgcttgtcc ttcatcactt aatatataat ctattaaagc
     4681 tttacctttt tctgacaaac catcttcttt attaactaat ataaatggtc ttgatattgg
     4741 atatttatta gctattacat ttgcatcagt tgcttcaaca tcatttattt ttaaactatt
     4801 tactttctca tcaacatatc caaatgatat atatcctata gcattttcat ttccttcaac
     4861 tgtagttttt atatttcctg aaccatcact tatttgtgca tctttagtta gttcttctga
     4921 atcaaaccct acaatttctt ggaatccatc tctagttcct gaaccttctt ctcttgatac
     4981 tattactatt ggtgcatcat ttcctcctac ttctttccag ttagtaatct ttcctgtata
     5041 tataccttta acttggtcca ttgtcagatt ttttactgag tttgcagtat tagttatcaa
     5101 tgctatacca tctattgcta ttttagtttc ctttaatcca gcactttttt cttcatcttt
     5161 taaatctctt gaactcattc ctatttcaga tgttccttct atagcatttt ttatacctcc
     5221 agaagaacca atctgttgta cttctacact aacaccttga tttttttctt gaaacttttc
     5281 tccgatagtt tccataagag gacctattga agttgaacct gaaacagaaa ctttgcttga
     5341 atctcctgaa tcacctgaac ctcctgagtt agaactacat cctactgcta aagctccaac
     5401 taacattgta ctagctaaaa gaacgcctat tttcttttta aacattttac ttacccccgc
     5461 ttatatttta attttttgtt gtcatttttc tatattttaa taatacagat tctttgttaa
     5521 atgaatatta gagcagggtt aagtaaatgt aaatattgtt aaatacatgt aaaaacttaa
     5581 ttttatatga tttattctta attagattaa ctttttaaag ttactttagg cagatataaa
     5641 cataatacaa ctcatattat cagaagtctt cattgaagct atcaacctat cacatcctat
     5701 aattcttttt ctagtttatt aactaattca gcaaatacat ttaaggcttc ctctatagat
     5761 tcttttttcg tcatatctac accagcagat tttaattgtt ctagtgggta ttctgaccct
     5821 ccacttttta agaagtttat atatctatct actgcagtac ttccttctga aagtatctgt
     5881 tgacttaaag cacttgcagc agaaaaccct gtggcatact tatatacata gaagtttgaa
     5941 taaaaatgag gtattctcgc ccattctatt cctatttgtt tatctactat tgcagatttt
     6001 ccgtaatact tttcatttaa tttgtagtat atattagtaa attcatcagc agtaagtggt
     6061 tctccactct ctaccctttg atgtacaatt ttttcaaatt cagcaaacat agtttgtcta
     6121 tgaactgtag ttctaaattg ttctaaataa taatttaata agtatatagt ttcatcctta
     6181 gacttagaat tttctaataa ataatttata agaagtaatt catttaatgt agatgcaact
     6241 tctgcaacga atatcttata gtccgaatat aaaaatggtt gacttgttct tgaataatga
     6301 ctgtgaacag agtgccctaa ttcatgaatt aaagtgaata gagaattaag gtcattttta
     6361 tagctcatta atatataagg atgtgaatca taacttcccc aagaataagc tcctcctttt
     6421 ttaccttcat tatcatatac atcaatccac ccttcatcaa aagcactttt tatgaccttc
     6481 aagtattctt ctcctaaagg tttaagtgcc tctaatatga tatcctgtgc tttatcatat
     6541 ggtatatcca tgtcaaattt atctgtcaaa ggaacataca aatcatacat gtgtatttca
     6601 ttcaatccta aaaatttctt tttaagctca acatatttat ttagattagg aatactttta
     6661 tctatagctg atataagatt attgtaaaca tctaaactta catcatctga aaataaagac
     6721 gcttctattg ctgaattata ctttctagtt tttgcgtaaa atatctctga ttttattcca
     6781 ccataaagcg ttgaggcaaa tgtattttta tatttctcat atgttgaaaa ttcaccttca
     6841 aaagcgtctt ttctaactct actattctta ctttttataa ataaagagta gtttgaatga
     6901 gaaagcttaa ccttattccc ctcctcatct tctatctcag gaaattccat atctgcatat
     6961 gctaacatat cgtatacatt ttctggaatt gtcgataatt ctgatgtagc tgcaagtatt
     7021 tcttcctctc gttcactcaa agtatgtggt ttatctctca atatatcctt tatatatttt
     7081 gcataaaagg atagcttctc atcttttaag tattcactta acttattctc ttctatcgcc
     7141 agtatttctg gaactatata agaagtagcc acacctaaat cagtagataa catttctgtc
     7201 ttagtggctc ttccttgatt atcattaatt ctagtatctt catgttgttt catatgagta
     7261 tatacatata aattttgaac aatcctagat gcattttcag agatatttaa tactttatat
     7321 aagttctcct tactctcaga aagcttccct ttatattctt ttacttcttg gataagattt
     7381 tttactttac ttatgtcttt ttcaatttcc tctttactgg agtacatctt atctatcttc
     7441 catttgtact tttcttctat tgtacttcta tcagttccca tatcatacac ctctatttta
     7501 atttgtttta tattttattc actttacaaa atatattgta tatattttag tccaaataat
     7561 ctacatttat tactccttct atcatattta tttctttcat aacgtcgtgt aagtgtattc
     7621 tcttagcata agatacaaaa aaattaacct tccagtattc ttcattacga cattctattt
     7681 ccatatttct tacgataagt tgattttttt caaatttatc atataaaaaa ggtatagaat
     7741 ccaaattgct taattttaat atcaatacat tcttttcttg gctaaataaa aacgtattga
     7801 ttatcctaag cacaaataat gctatcatta caaataatcc acctagaatt gccaattcat
     7861 aataaccata tccaactgct ataccaatac atgcagttgc ccacatccca gcagctgtag
     7921 taagcccttt tactccacct ttatttttta aaattgctcc tgcacctaaa aaaccaattc
     7981 cagataatac ttgagcaggc attcttgctg ggtcagcatt tgtatacaaa ttataatcaa
     8041 agaataattg aatcgaagtt attgaaacaa tacaagaacc tattgcaatt agtatatgtg
     8101 tcctaaatcc tgcaaactgt cttatttttt ctctctctaa gcctattatt cctcctatta
     8161 ttagtgctaa tagtaacctt attgctatct ccttacttcc tatatcaatc ataatataaa
     8221 accctcctga cttaattaca acttatgtcc tacagataat ttacacatca gaaatactat
     8281 tttatatctt aatattatta ttaatatatg taaaatatag ccatctgtta atttttgata
     8341 caaaaaaacc cacaactgtg ggctatttgc ttaggtacat gtatttattt attaatatat
     8401 ctattttctt acttgattct actaactctt cttgaaatat ttctggatat tctatgtatg
     8461 tatttatctc ttccctaact gcttcaatct cattttttaa cgcttctaat tcattgttcg
     8521 ccataataat caccactatt acaaatttgt atatttttat atacatttta tcaaacaata
     8581 ttatattttg tcaatattat gtcaataaaa cttactataa aaatgcttta atttaggtat
     8641 tgtatacctg gatactaagc ctacaaacat accagttact aatgatgcca ttaacataaa
     8701 tggtaagtac cctatagcaa gtatattttc tatgattaaa cttgcaacta ttaattgacc
     8761 tatattatgc cctattgcac ctataatgct tatccctatt agtgataaat cttttatttt
     8821 actcgctaaa tacataaata aaagactcag ataagctcct cctatactaa atagtagata
     8881 tgataaaggg cctgcaaata ttgatgacaa aattaccctg atagtaacaa ttataacagt
     8941 atctttgaca cctaacaaca ttaatgaaat taaagttata atattgctca aacccaattt
     9001 tgcccctgga aatataacta taagaggatt tggtatatat gtctctaata catataaagc
     9061 taaactatac cctatcataa gacctaaaaa tgtcattttt ctagttttca ttaagttaac
     9121 tcctccaaat tttatctatc atcttacatt ataatacaaa atatataaaa acacctaatt
     9181 tagactaaaa tatttttaat ctatttagaa taaatattag ttatttcaat gtgcaattac
     9241 ctctataaat cttgactttt tgtatcatac ttgttacaat tttaatgata atgaatttca
     9301 ttaggaggtg ttaataatga ctgtttacga tttaaaaata ggagaaaaag gatttattaa
     9361 tagtatagat ggagatatta aattatctaa aagattgctt gcacttggat gtataaatag
     9421 tactgaaata gaagttaaaa aggttgcccc acttggagac ccaattataa ttagatttag
     9481 aggctttgac cttgcgatta gaaaatctga tgctaaaaat atttctttac aataatagag
     9541 gaggagctac ccgtgattaa tgtagcatta ttaggaaacc caaatgtcgg taaaactact
     9601 gtttttaatc tacttacagg ttcaaatcaa tatgtaggta actggcctgg tgttactata
     9661 gaaaaaaaag aaggatttct tggaaaagag ataaaggttg tcgatttacc tggtatatat
     9721 gcaatggata ctttctcaaa tgaagaaaag gtttctaaat catatttaga aaatgaagat
     9781 gttgatgtaa tagtaaatgt agttgatgct tctaatttat caagaaacct ttacttaaca
     9841 actcaactta tgcaatttaa caagcctata gttatattgc ttaatatgtt agacattgca
     9901 gaatctaaag gagttaatat agatgccaaa aaattaagtg aagaacttgg agtaattgtt
     9961 gttccaatta tagcaaaaaa gaaaaatggt gttgatagga tagaagaaga gattaaggga
    10021 gctacgcaaa aagcttttat atatcagaaa aactttggaa gtgaaacaga aacatataaa
    10081 aaattagaat ccattttatc gagatgtaca aaaactacat atacacaaaa gaaatctatc
    10141 agtgataaga tagacaatat agtcttgaat cctatattag catatccgat atttattggt
    10201 gtattgttcc tcttatttaa atttaccttt gactgggtcg gaggaccact acaagaggga
    10261 tttgctagtc ttatagaggc ttatatttct gcacctgtta gtgatatgtt atctaactct
    10321 agcccatggt ttaaatcact tatagttgat ggaataattg gaggtgttgg tggtacttta
    10381 ccattcttcc ctttaatatt tacattattc tttggtatat cgttgtttga agatagtgga
    10441 tatatgtcaa gaacagcatt tttaatggat aaagttatga gaaaggttgg tctatctggt
    10501 aaagctttta tacctatagt tatgggtatg ggatgttctt ctccagctat aatggcaact
    10561 agaacattgg aaagcgaaaa agatagaaga gttacagctc ttattgcacc acttatgact
    10621 tgtggagcaa agctacctat atatgcacta tttgtagcta tattctttcc acataatgct
    10681 gctttagtaa ctacatcact gtatttggtg ggtatagtta tggcaattgt agttgcatta
    10741 ttcttaaata aaacagcctt taaaacagag gctgaaccat ttatattaga attaccagaa
    10801 tatagaatcc ctactataga tgcacttatt aaaaatactt ggaataaatc aaaaggtttc
    10861 ttgataagag ttgttactgt catgtttgca atgtcagtcg taatttgggg actttcttca
    10921 ttcaatgttt ttggatatac agaagatata aatgtaagct tcttggctac tctaggtcat
    10981 attatatctc ctatattcaa acctcttggt tttgatgatt ggagaacttc agttgctatt
    11041 ttaagtggtc ttggagcaaa agaaatcgta gtaaactctt taaatatact atatggtaat
    11101 ctaactgttg tcttaccaac aatatttaat ggagttactg catatacatt cttgatattt
    11161 actgctttat atactccatg tatagctgca cttgcaacta tgaaaaaaga atatggcaat
    11221 aaaatgatgt ttacatcatt tgcatatcaa tttatactag catggataat ggcttttata
    11281 gttaaaagta taggtggagt actatttatg ggtaattcaa taatagaagc tttgataggt
    11341 ggaataatta tagtccttgc actctttatt ctgtttaata aattcaagtc tacaaaaaac
    11401 ggaaatgtct gtagtggatg ttctatttgt tctggatgtc caagtgctaa ttcttgctca
    11461 agtaaggaag acagcaataa aaaaaataat gcacactaat atataaatat tttagtttaa
    11521 aataagataa aagtgggtgt ctcaaaatta actttttagt tcatgaggca ctctttttta
    11581 tagaaaatca aatatatttt catcatttca acagtaaaaa agagcttatc tagtaaaaaa
    11641 gaggcttatc tctattttga gacaacctct ttttttgtta cttattcagt ttcttgctac
    11701 ctaacttcat gtggaaccta gtatcttttg gaacacttgg gtctacacct cttaatgtag
    11761 gtatttcccc acatattagt tgtataggta ctataaaatt aaatgtttga tagtaaggat
    11821 tatctgtgat atcaatcttt aaatttttat cattttctgt aacatctcta cctatagcaa
    11881 atacattttc cgtccaacca gaaagtacat caatcatctt agttactcta ggttcttttc
    11941 ctgtatctag tataaatata gtagaatcac tatttatagc attatatata ccatgtataa
    12001 actcttcaaa ctcataccca gttacaggta ttctcattgt ttccaataat tttaatgctg
    12061 cttctaatgt atctccatat atatcagagt gacctattat tcttatttct ttagaattaa
    12121 ctaacttttc tttgtttctt tcaatccatt gtttagagag cttatacact gcttcaaatc
    12181 tattaattgc gtctaatatc ttatttattt cctcattgta cttttcacta gatattatac
    12241 ctttttctct agctatttga agacctaata acattaagtt taactttgta caataatatc
    12301 cttttgtttt agctccagat ttttcttcac cacaatttac tgttaatata taatcagaaa
    12361 tctcatctat taaagcattc ttacaacctg ccatagatgc tattttacag cccttatctt
    12421 cagctaactt catagcgttg tatgtagaat aactactccc accttgagat acacctacta
    12481 ctagggtatt ttcattatca aacttgaatg tatcttcagt tatcataaat ggatacattt
    12541 tcacaacatc tatatctaat aaattttgta aatatggttg tacttgcact cctgagtgat
    12601 agctagtacc tgaacctgtt attataatct tttttagatt tgttctctta acttcattaa
    12661 ataagctatc tgcattagat ataatttctc tcattctaac tggtgtttct aacatataat
    12721 cttgtattgt cattttatac accttcctta tctatatatt caataaatta taattaattt
    12781 ctataacaaa cctatagcat gtaaagcgat tcctgttact atagtgccta tagttaattt
    12841 tgttacactt acctttctct ttaacatgta gtatattaca aatgcaaagg ttaaaggtaa
    12901 catattaggt attattttat ctagtatatc ttgtaaaatt acttctgcac catttaagtt
    12961 gaatttcaga ggagtagtta tactcaacat agttgcaacc attcctccta caacgaataa
    13021 accaacagtt gtagccatag acattatttt ctccataagt ccctctcttt gtattctttc
    13081 aagagaattt acccctactt gataaccata cttaagtcca aatattctaa gggcaaatgc
    13141 tggtatattg tataatagta agaataatag tggtccaaag atattacctt cttttgcaag
    13201 tgatactcct actcctgctg ctataatcct aaatgttccc cagaataatg aatcaccaat
    13261 tcctgcaaga ggtcccataa gagctgtttt aacagcacta attgaacttt catcaaattc
    13321 tggattatta gcattctctt cttccatagc tgctgttata cctaatatag caggaactac
    13381 tactgtcgtt gtattaaaaa tctccaaatg cctttctatt gcttttgctt gattttcttt
    13441 cttattgtat agcttcttta ttgcaggtaa cattgaataa cagtaaccta agttttgcat
    13501 tctttcgtaa ttaaatgcac cttgaagagc aaatgaacgc caaaacatac ttcttaattc
    13561 tttttttgtt aatttattca aagtcaatct ctccttccgt taaagtattg attgctaatt
    13621 gaggattttg attattttct ttgttattga tataaaagtt tagtacaaat gctacacata
    13681 ctccaaataa agctatagca gttatatcta atccaccata agatgctaac aaaaacccta
    13741 taaagaaaaa tacagacatc ttttttgaaa gtaacatatc cataagcaat gcaaaaccta
    13801 ctgctggcaa caatgttcct gcaacaccca gtccgtctaa tataacttta ggtattgcat
    13861 ttatcaatgc tgtaacttta tcagcaccaa tcataattgc taaaaaggtt ggaactgatg
    13921 tacttaaaaa gaataatatt actggcaacc acatcattat ggtaactttc ttaaaatcag
    13981 cttgtttagc atagaaaact gccttttgag aaaagtacga atttattatt ctaactaata
    14041 ctccaagaga ttgtgctaag actgctactg gtactgctac tgctaaagca acttctgcac
    14101 cattacctga taatatagca aaagctgtac ctaatacacc tcctgttact acatctggag
    14161 gagtagctgc acctattcct gcaattccca tccaaattaa ttcaagctgt gcaccaatca
    14221 taattcctga ttttatgtct cctaaaacta atcctactaa catacctgta actattggcc
    14281 tatcaagcat agattgacca aatatacgcc catctagtat accaactcct gctataatag
    14341 ctaacatagt agcttgtact aacatattga acctccattt taacttagtt taatttacat
    14401 taaattacat tacatttttg attttgtttt gattttctaa taactcattt acataaacaa
    14461 ttccattttt tgcttcctct aatcctgatt taactattag ttttgtatcc tcctcaacta
    14521 ataaaagtat agataaaagc attggtaaat ttactcctgt caataattca aatttgaacc
    14581 cttgtaaaag taagtttgta catatgtttg ccacacttcc accaaaaaca tctgttaaga
    14641 caacaaattc ttcactatct ttattatttg ataatatact tctaatttct ttctcagcat
    14701 catctgctga tttatccgtt gtcatacaaa aatactcaat attagttaat gaaccaatta
    14761 ttaatttact tgtgtcaaca agctctctgg acaattctcc atgtgttgca attagaaatt
    14821 ttttcattct ctttctccta aattatttta ttaaatctga tagttgtttc tttttgtctc
    14881 taggtaacat tctaaattct atatcaatcc ctaaatcacc tatttgttta aagttattta
    14941 tatcatcatc atcaacagca actaaatctg taagtttctt cttcccttcc tcatatctta
    15001 atcctcctac atttatttct tttaatactc ctcctgaatt tttagctaca actaaagcat
    15061 ctttggtatt ttttactaac actaatacat tgcttttctt tgaagaagca aatttttcta
    15121 ctatttctcc agattcctct acccccctta tatataaagt tacattgcta ggtttcgcta
    15181 agttcagtga catagctttc atttggtcat tttttgcttc atcatttgca actagtatta
    15241 catttatacc tagtgatgtt gcccatccat atgcaacctg accatgtatc aatctgtcat
    15301 caattctcat caaagatacc attttcccca aatcctccat catacattaa aatttttatt
    15361 gtttatactc tcatttatct ataaaagcaa aaagtatgcc aaatactggc atactttttt
    15421 aaatttcatt cacttatttt gtaatcttgt attttactgt ggtagaccat agataatatt
    15481 ataaatatat cctatctctg aaattggtat ttgcacacta taactctttt ctagcacact
    15541 aaatgcattt tttattagtt ttacttcact atgatggttt ttgattaaca aatccatatc
    15601 agtatactct tcaatttcag tttgtctaac taatctttca atcatacaac ttaagtgaac
    15661 atataaagaa atctttttat catttgaaag ttttttcttt tgaatatttt ctatagcatt
    15721 aattccttct tctattctct ctattatctt ttttgaatct aaaatagtta gactatctat
    15781 tactctaatt aatgaaaaat tctttacaat agaatcattt atgttcttta ttttttcatt
    15841 ttctactact ctgctaaata ttctaaaaac atcatcttct ccttttcctg atattatatc
    15901 ctctaataat ataaaatcta ccccattcac atttggattg gcagttccta ctatagccat
    15961 cacatcatac atttcaaaaa ctggttcttt atctcccata gaaacaagac tatcataatc
    16021 ataaggtatt attttgacat caactattcc aattaaactt tcggaaagta gatgttggat
    16081 ttttatagca gtacctatcc ctgtaaaaca agatgatatt atagcatatt ctttattttt
    16141 ttgaggataa tacaattgta tattaggttt agtttcttca tccataattt ctgcaatctc
    16201 ttcaatgttt ttaccttgca ttaataaatt tcctacttct agtgcagaaa tagtggatat
    16261 ttggtctata aataacattg ggctatttat cttattcttt aaatcttcat atataagatt
    16321 aagagagccc atatcaaata acacaattaa accattcttt atcctatctc cctctaggta
    16381 tctagacatt tgttttgata catccgcaat agtagcatct ataggcatat caatagctgt
    16441 aaatatattt ttacccaaca tccgattaca tacattcgcc atactactgg cagtagcata
    16501 tccatgtgct ataatagtcg cattaacttg attaaatttc attcttataa ttgttgattt
    16561 aataaatata atcattatga ttatatctac attattaaga tacatatcca atctattcgt
    16621 tattaatgtt gatattttac ttattataga gtattctttg taactctctt tttttaaata
    16681 tattagtaat ttattttcta aagctttata ctcatcagac aattgataag attcgtcctt
    16741 caaataaaga taagacgaaa atgccaatac attattccca tcatatttta tattgttatt
    16801 aacttctaaa tttctaaata tatcctgcat tatcgatatt ataagctcca taagtacgtt
    16861 attattttca tcattatttg aaaagactaa attgtccatt agtctaatca ttagattaga
    16921 actcttttta ctaaattttt cctttgttat ttcatttttt tctagtctat caaagtaatc
    16981 taaacatttt atgaaacttt gatagattat atttctttta tttcttccat ttgacatttc
    17041 aatactaaat tgtttaggag aaaagataat gcttttgctt ttaccacttt ggaattgaaa
    17101 tatatctaca ttttctatat aaagatttct aggaaggtct tgaagagtaa caaaaatatt
    17161 tccatttctt ctttgatttt ttgcataagc tcgtccacat gcatatttta ttacattttt
    17221 acattcacct atattcccct tgaattttat ctttaaaatc gcatttaatg taatttgagt
    17281 tatctctata ggcaaaccaa gtaccttact ttcttcataa ataaaatgtt ctacaatctt
    17341 tcttttttca atagtccctc tttcttctat attgggaaca taaatattta tgggtattct
    17401 tcttaaaaag gtttgtaaaa attcagatag actttctgtt gtagcaaata ttaatcttac
    17461 cctagctttt ctaattactc cattctctcc aatcctagaa aacgtacctg tatccataaa
    17521 tgtaaataac ttttcctgac cttcactatt taatctatgt acttcatcta aaaataaaac
    17581 accaccatct gattgttcta ttagacctgc tctagattca tttgctccag tatatgctcc
    17641 ttttacatgc ccaaataata gactagacaa taactccaaa ttattataat actgcgcaca
    17701 gtttaacaca ttgtatggag aatttttttc taatacatta ctgcttacac tatatttgta
    17761 tattatcttg gctaaaaaag tttttcctac tccactttca cctgtcagca ttattgtaag
    17821 tccgccattt ggataaagta cagcggtttt tatttgttcg attacttctt ttaagctatt
    17881 atcatatcct atgatactat taaacacagt ctcttgcttt aataatttac actcattttt
    17941 aagttcatct aaattttcat aaatactttt ttttgtaggt cctactacat catttatttt
    18001 atttttaggt aaaaaaataa caggtctact gtttatcttt ataagattat cagattttac
    18061 taaatcattt aataatctac ttgattgagt tctacttaaa ttaaactttt ttactatatc
    18121 ttctgttatt atgccacttt cttttttaag ttcatcaaag ttgttaaata atacacattc
    18181 atttacaaaa cttaaaattt catccctcat gttcaacctc caatacaaat atcaataata
    18241 ctataatatt agtatatcat ttatatattt atgaaaaaga taattatatt cctatcttga
    18301 ttctcttttg taagtttgta aaatcaaact ttttataagt ataaaatcta gattttttaa
    18361 cataatctca aggttcttat ctttccata
//
LOCUS       DAYASV010000073        18315 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYASV010000073 DAYASV010000000
VERSION     DAYASV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18315
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.073"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(357..1079)
                     /locus_tag="ACGI68_002387"
     CDS             complement(357..1079)
                     /locus_tag="ACGI68_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFH0138822.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
     gene            complement(1138..1815)
                     /locus_tag="ACGI68_002388"
     CDS             complement(1138..1815)
                     /locus_tag="ACGI68_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFH0138823.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            complement(1843..2658)
                     /locus_tag="ACGI68_002389"
     CDS             complement(1843..2658)
                     /locus_tag="ACGI68_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0138824.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     misc_binding    complement(2884..3094)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3812..5878)
                     /locus_tag="ACGI68_002390"
     CDS             complement(3812..5878)
                     /locus_tag="ACGI68_002390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0138825.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(5955..8984)
                     /locus_tag="ACGI68_002391"
     CDS             complement(5955..8984)
                     /locus_tag="ACGI68_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0138826.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(9037..10497)
                     /locus_tag="ACGI68_002392"
     CDS             complement(9037..10497)
                     /locus_tag="ACGI68_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFH0138827.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(10589..10963)
                     /locus_tag="ACGI68_002393"
     CDS             complement(10589..10963)
                     /locus_tag="ACGI68_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138828.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(11139..11879)
                     /locus_tag="ACGI68_002394"
     CDS             complement(11139..11879)
                     /locus_tag="ACGI68_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFH0138829.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(11900..12688)
                     /locus_tag="ACGI68_002395"
     CDS             complement(11900..12688)
                     /locus_tag="ACGI68_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HFH0138830.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(12776..12866)
                     /locus_tag="ACGI68_002396"
     tRNA            complement(12776..12866)
                     /locus_tag="ACGI68_002396"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12830..12832),aa:Ser,seq:gct)
     gene            13125..13889
                     /locus_tag="ACGI68_002397"
     CDS             13125..13889
                     /locus_tag="ACGI68_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0138831.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(14170..15285)
                     /locus_tag="ACGI68_002398"
     CDS             complement(14170..15285)
                     /locus_tag="ACGI68_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFH0138832.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLIFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(15326..16852)
                     /gene="glpK"
                     /locus_tag="ACGI68_002399"
     CDS             complement(15326..16852)
                     /gene="glpK"
                     /locus_tag="ACGI68_002399"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFH0138833.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            17145..18242
                     /locus_tag="ACGI68_002400"
     CDS             17145..18242
                     /locus_tag="ACGI68_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0138834.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRY
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
BASE COUNT         5908 a         3261 c         1952 g         7194 t
ORIGIN      
        1 ttataagctt acttgttata aaaataactt ttataattta tttcaaaaaa aatattgatt
       61 tttgtgtcgc attttgttat aataaattaa ggttatcccc ctggtaaccc tagttagtta
      121 atatatctat tcccaatacc ctttcataag gatatatttt atatttatta aaaatcggat
      181 tagtaacgga aattaattcg gtggaaaaaa agagcttacc ccgagctctt ttttttctgc
      241 aaaaaatcgt aaaaataaag ttaatattaa tataacttta attttaaaat taatattaac
      301 catcactaaa aaaggatgta ttgaatttct aatatcaata acatcctttt tatatattat
      361 agcacttttg ctaaaaatgc ttttgttctt tcatgttttg gatttccgaa aacttcttct
      421 ggagtattat cttcaagtat acttcctcca tctataaaaa taactctatc tgcaacttcc
      481 ttagcaaatc ccatctcatg agttacaatt gccattgtca taccttcttt agcaagttct
      541 tttataacat ccaaaacttc gtttaccatt tctgggtcaa gagcagatgt tggctcatca
      601 aacaacatca tatctggttc catagcaagt gctctagcta ttgctattct ttgcttttgt
      661 cctccagata attgtgatgg atatgaatct tttttatcta aaagaccaac tctactcaac
      721 aaaatttctg cttttttttc tgcttcttcc ttagtcatct tttttaattt aattggtgct
      781 aatgttatat tatctaaaat agtcttatgt gggaataaat taaagttctg aaacaccata
      841 cctatatttt gtctaacctc atcaatatta gtttttttat cgaccaaatt ttttccttca
      901 aatattattt ctccacctgt aggtatttct agtaaattca tacatctaag aagtgtactt
      961 ttaccagaac cacttggacc tactataacc attatttcgc ctttagcgat ttctaagtct
     1021 atatttttta atacatttaa atctccaaaa gatttactta aattttttat tgtaatcata
     1081 ttatcacttt cctttttact attctatgtt ttccctaaat gctaaacatg ctctcatcta
     1141 gttagacaca gatagtttct tttctaataa tccaactaat cttgatagag taaatgttat
     1201 aacaaagtaa attgctgctg ctactattaa tggctctaat gctctaaatg ctgcattttt
     1261 tactatatca gcagtacgca ttatctcaac aactcctatt acagatatta ttgaagattc
     1321 ttttacaagt gtaataaact catttgctaa tgaaggtaat atatttttta tagcttgagg
     1381 tattattata tatctcatag ttgaccaata atttaatcct aatgatctac ttgcttccat
     1441 ttgtccatta tcaactgatt gtatacctga tctaagtatc tctgccacgt atgcaccaga
     1501 gtttactgat aatgcaaatg cactagctat aaattcactt ggtataccaa acaacatagg
     1561 gaatcttatt cctaattgag gtaaaccaaa ccatactata tatatctgta ctaataatgg
     1621 tgtccctctt aaaacctcaa tatagattga tgatattgct cttaatacct tgctctttga
     1681 tatttttgcc atacagataa ttatacctat tataaatcca aagcataatg ccacaagaga
     1741 aattgatact gtcacacctg ttccttccaa aaaagaagtc ccaaatctac ttaaaaaact
     1801 aaaatctaaa ctcataattt catctcccac ataactttct tactaatttg aagcagcttc
     1861 agtagatgct tcatttaaaa attcttctat cttcttgcca tcttgtaatt cttttatctt
     1921 tttgtttaat aattctataa aatctttgtt attgtcagat tttttgattg ctgctgccat
     1981 accttctgtt acatctttcc caacttcagt atttgccatt tttattccat catatttttt
     2041 aacatttatt aaagaaactg cttcattagt aacaactgca tctatgtttc cattttttaa
     2101 ttctgtaatt aaatcaggta ttgctttcaa agattttgta gttgtcattt ttaaagtgtt
     2161 aactacatat tcttcttgga tacttcctgc ttgaactcca actctaagtt tctttaaatc
     2221 atcttcagtt tttactttgt ctatatctgc atctttaact ataactgcat ttctacttaa
     2281 ataatataat tctgaaaaat caacactctt ttctctctct ggtgttggag acatacctgc
     2341 aagaacaata tcaacttggt ctgcatttaa agcacctatt aatccatcaa atgacatatc
     2401 cttaatttca acctttacac caagctcttt tgcaaattct tcagccaaca ttatatcgaa
     2461 tccttttatt gaatcttttc catctactac cttatgaaat tcaaatggag gaaattctgc
     2521 actagtacca acaactaatt ttccattgtc ttttatttgt tctaactttg ttttttctcc
     2581 tccaccacta catcctacta gcgataatgt caatcctaat actaatccta aacttaataa
     2641 ttttttaaat actttcattt taactacccc tctcttttaa ttataatttc cctcagattt
     2701 taatacttaa atgtttcgag ttacaaaaaa agtctctcag tctttaatga ctaagagacg
     2761 actttaccgc gttaccactc ttattgataa atacttatat tactttcaaa atttgtgact
     2821 gttagcattt aagctgtatg gatactttta tttttaaatc aaaaaagtcc cttagcttta
     2881 cagctaagag acgaattata tccgcgttac cactcttttt gataagtaag tacttatcct
     2941 ctcaatccct taaggcgggt aaaccaatta tcatactaaa gtttctgata atctcctcca
     3001 gagctctctt cacatagact tcattgctag gcttacacca acccctaact cgctgtaaat
     3061 ccatatatgc tactttctct atcattggat ttgtttcctt aaattgttaa tacaattgta
     3121 cttccttata atatttttgt caataagttt tttatttttt taataatttt tacttgtaaa
     3181 ttatctaaat actactgttt atatttattg atattagttt tgtaattata aatagtatat
     3241 ttcatatttt tagatatttc ttaaataata ctaaataata tctttcacat tgttgctttt
     3301 tcttaaacaa tactaaataa tatctttcac attgttgctt ttcttaaatt acactaaata
     3361 atatctttca cactgttgct ttttctttaa taatactaaa tagtatcttt cacactgttg
     3421 ccctttcttt aataatacta aataatatct ttcacattgt tgctttttct taaattacac
     3481 taaataatat ctttcacact gttgcttttt ctttaataat actaaatagt atctttcaca
     3541 ctgtttctct ttctttaata atactaaata atatctttca cattgttgct ttttcttaaa
     3601 ttacactaaa taatatcttt cacattgttg cttttcttta ataatactaa ataatatttt
     3661 ccacattgtt gctttttcta aacaactata aataatatat taaaccctta tttctttttc
     3721 taataagtta aataatacat tttacacata tctatttgtc ttgcacataa taaataatac
     3781 attatatttc tatctaaatt tagattctaa attaaatagt ataaattata ttcttcaata
     3841 aacaagtttt caaatccaag attcgctctc tatctttaaa ctttattgta aacataattc
     3901 catctttttt aattatttct ccttcatgaa attttttatg gtatacctta tcccctacac
     3961 caatactgtc aatttctttt ttcgttggag attttatatc attaataaat aatgatttat
     4021 caactttctt accatactta tttaaagtag agcttataca taacttttct tcagctcttg
     4081 taatagcaac atacataagt cttctttctt cttctaattg ttcttctttt ttctcttcct
     4141 tacaaatatc atatgatttt tcatgaggta ttgttccttc atttacacct ataatataaa
     4201 catttctaaa ttctaatcct tttgcactat gcatagttgt aaatatgaca ccctcagtct
     4261 gcttattctt gttattttcc gttaactcag tctttactct atcaatatgc tctaaaaact
     4321 ctgtaacagt attaaaatta gttgctgaac tctcaagttc atttaatatc tctacaagtc
     4381 cattagtctt tatctttctg ttgctacaat aatctaaaac atatctatca taatcaagac
     4441 tagttctaat ataagatatt gcatttttag gatttaaagt atttaaataa cttaaatcta
     4501 tttccaaatc atttattgtt ttaacttgtt taggatgaag attacactta tttattaatg
     4561 ctgttataaa gtctttttca tccttaacca tatttatact gtctttggat atatatctaa
     4621 aaggcttgtt tataattctc aaccaatctt tatttgattt tggattaata cctattctta
     4681 aataggctag tatgtcttga cttgcccaat ggtcatatat tgtaattact gagtctttta
     4741 ctacaaatgg aatcctcata tccataaata catctactaa tgctcttgac tgtatattag
     4801 ttctataaat tactgcaaaa tctgaatact ctacataatc tttttgtatt tcgtcaatta
     4861 tttccctggc aatatataag gcttcttcct ctgaatcatg cggagaaata taggtaacac
     4921 ttcctccatc cccctgacta cacttaatga ttttttcata tctattttta tttttgtcga
     4981 taagtctatt tgcagtatga actatctcac ttttagacct gtaatttata tctaatatta
     5041 tttttttagt attattaaag tattgttcaa attcaagcaa aaaatctggt ctagcacctc
     5101 taaatccata tatactttgg tcttcatctc caactgcaaa tatattgttt aatgggctac
     5161 aaattaactt taaaacttca aactgtactt tatttatatc ttgaaattca tctatcaata
     5221 tatatttata cacatttcta accatttcta gtgcagactt attgttcaaa agtaaataat
     5281 aagttcttat aagcatatca tcaaaatcta ttttattaac tttagatttt tgttcttcgt
     5341 ataagttgta tacttttaaa aactcatctt ttgttaatac ttctgaatta aattcatttt
     5401 tatccataag ttcatttttt acataggaaa tttcatttat aacttgtcct atattctcat
     5461 catcatcagc attctctata tttaaacttt ttaggattgc cttgattgtc attcgctttg
     5521 acttttcatc aaatatactg tctaagttgt atctttcaaa gtatcttaga attttaaaaa
     5581 atacagagtg aaaagtacca taggttacct tattaagtct tatgtcatca cttaaattca
     5641 aagctctatt tttcatttct attgaagatg ccttggtaaa acttattgcc aagattcttg
     5701 ttggtgctat gtttttgttt acaaccatat tggctattct atatgtaatt actctggtct
     5761 tacctgaacc aggccctgca agtatcatgc aaggcccatc tatatgctca actgcttcta
     5821 attgattttc atttaacttt cttatatcta tatttgttgt gattattagc tccaccacct
     5881 tgatttataa ttatattatt ttctttttaa ttataaacta aaagttcata aaaataaatt
     5941 taatttttcc tctattaaac cacatcattc ctattttcat gacatatctt gagttctttt
     6001 ttaattttgt cgcttatatc ttcaggtata aatgatggtt tactaatata gtagccttgc
     6061 atataatcta ctccaagatt aattagtgtt tccatttcac cttttgtttc tacaccttct
     6121 gctatcactt taatatttct ttcgttagaa tatgagataa gattttttaa tattttctgt
     6181 ctgttttcat ctttatcaat acctcttaca atagacatat caatctttac atagtctggc
     6241 attatatata atagtacagc atctccgttg taacctgtcc caaagtcatc taaagcaatt
     6301 tctatattcc aatggtctat atatgctttc tttttctcaa taaaatcttc attaatctta
     6361 tcattctctg ttatttctag tactactcta tttaaatatt caccatataa atcttcaatt
     6421 tcctcaaaat ctacttttga caaattatta ttaggaatag aatttaagaa cagtttgcaa
     6481 tcgccaaatt tttctttgta ttttttaaat gatttaagtg ctttaaacca tgtaatacgc
     6541 tcaatttgat ataatttaga ctgtgaacga gctaatctaa tgacatctat tggcgaacta
     6601 aaagttttta ttttaggtct cataagtgct tcatatgcaa aaactttacc tttacaatct
     6661 acaataggct gaaatgcata ctcaactaat tgaccatcaa ttaacttgtt taattcttct
     6721 ctattactca ataagaatga atccttatta tatatattaa tatcaaattc actaacttca
     6781 cccttgattg tatttttaat ttgatacata gcaaaatctg cgtatctcat taattcatca
     6841 tagttggttg agtcatcagg gtaccaggaa attcctgctg atgcccttat tttaaattct
     6901 gttccatcat gaagttttaa taaggtatta ttcatctgtt tcttaacaga attaataata
     6961 tctcttatac tatctttact ttcatatcca tatataaata cataaaactc atcacctgaa
     7021 agtctagata ctataccatt ccatgatata aacttcttaa gtatgtctgc agcacaccta
     7081 atatattcat caccagcatc atgtccatat gtatcattaa tatattttag attatctaaa
     7141 tcccacataa taaatgctgc tatcttaagt tcatctttat tcttaaattt ttcttgtatc
     7201 tgtgaatgaa aagctctcct atttaaaaga ttcgttaaaa tatcaaaatc tctttcatat
     7261 tctatcttcc ttttctcaat aaattctttt gttacatcaa caacaacacc aagaaccttt
     7321 agctcatctt caaagatatt cattcttatc cacttaagtt ttttatcttt agattcaaac
     7381 ttatatatat atgtattttc tgtttcataa ctttcttgca aatatttatc tagtcccctt
     7441 aattttttca ggaacttatt tttttccata tatccatatt cttcaaattt gatttcagta
     7501 gtaaacacgc taaaaaattc ttttgtacaa aaaacattct ctgcgttttt gtataactca
     7561 aaagctccaa ttggcatatc taccatttca ataatctgtg ataattttga cgaagaatct
     7621 gcaacatttg cactgagcaa ctctatagct gaagataatt catctatttc agatatattt
     7681 atcttatcaa gatttactgg ttttgatgga ttactgtttc taactttgtt ggctaaaaga
     7741 acaattggtt ttgtaaataa tcttgatgca atataaatac ctattagacc tattaataaa
     7801 gaaattgcta tagatattat aactaatttc tctacccttt ttgaaaatga caaaagactt
     7861 ttttcttcaa taatacccaa aagagcccaa cgttcatttt caaatggagt attggagtta
     7921 tataaatcta agtattgtac actagcataa atgttgccat taccacattc attatcttta
     7981 tttttaatct tatatatatt tttatacgat tccttctgct ctaaattaga atgtaaacca
     8041 tcccaaaatt tttcttttaa tgagtatcca tttgaaatta cattttcaaa ggccatatca
     8101 ttatttctat ctacacctaa aatataatta ccctttttat tcccatttat ctctgagtat
     8161 ggaagtaatt ctcttaaata atttacactt aaatctattc caagtactcc atatacagtt
     8221 ccatcttcat aaataagggg aactgtatat gtcataagcc tatcacctgc atttgtcaat
     8281 aaaaacttac ttccccagta tcctaaatta gaacttgata catttggatt atcaattgca
     8341 gctctaaatg gtttataaaa aaaatcatct ccatcttgct cattttcact tttaaatgaa
     8401 aacttaggtt cccaataact gtccatagat attccaaaac ttcttgctat atctgatgaa
     8461 cctctctcaa ttaacaaatc agaattatca tttggattaa acttagggtc taaatccctt
     8521 atatacaatc ctgtcctact tatttcacct ttactatcta ttgacttatc gttaatattt
     8581 ttgtcactta ggattataaa agctcctgta acagaatttt ttctcaaaag atatattaaa
     8641 tcatttgata tgctttttat aatatctttg ttaatttctt cattgttgcc tacatcctca
     8701 attaaggcat ttttttcatt taatactttt tttacagagg aattaattgt agcttccgtt
     8761 tcacttgtat ttgaccatct ttgtaacatt tcattttgta tataattttt acgattaatt
     8821 actctttcat ttaagatgtc aaacgaatta tcatttaatt tcttaatagt tcctccccat
     8881 aaaattgtgg cacaaaataa acttgtttga attagcataa caataattag gggaaatatc
     8941 atctttttca ttattgaatt gttttttctt agattgtttt ccatacccat tccacctata
     9001 cctttgtgct cgtcaacaca ctaactgcca attcatctat ttttttatag cattttcaag
     9061 ctcagcctta aaactttcaa accaagtttt aaaattttta tcattattaa attgtgctac
     9121 tgcatccttc ttagaagttc cactttttac taattcattt attttatttg aatcttcttt
     9181 agctttatca agcattgaat attcaagtat ttgtctagct ttagttccac catcaaatgc
     9241 tttattagta tataaattac aactgttaat ctgttcaata gatgaggcta ttgaaccttc
     9301 aactttatca gacaaactca aattatcttt cttcataact tcttttataa catccaaatc
     9361 attcgttttt ttctttactg gtaagtatcc agaagcaata gagaatttta tattaggttc
     9421 tttatctgta aaccatttta aaaattctac tgatgcatat tcttcttctt tagtagattt
     9481 tgttacagcc attcctgcac cttgttgtac agcatatttt ccttctcctt taaaacgtgg
     9541 tgcttgaaaa acagatgttt caatattata actctcatta tcatttagca ttactctatc
     9601 tggaaagtat gctgctcctg aactagaacc tacaagagct aatatatcac ctgtttttgc
     9661 atcatcagaa cgaaacttac cataagaagc aaaatatcca tttatatatg gtacatagaa
     9721 attatcccat agttttttca taactttttc atcaatattt agcgtaactt taccatcttt
     9781 aacagaaaaa atttctactc ctagttgttt acttcctata ataatataat ttgctaaagc
     9841 atctcttcca aaaaatgctt taccatcatt aggctctttc gttaaactat ctgtccattc
     9901 ataatatttt tttgatgtct ctactaatcc ttcaattgtt tttatatcat ttatatttga
     9961 atttgtagct tttgcaaatt tatcccaatc agttttattt aacatcaaaa tttcagttga
    10021 ttttgcaatt gggaaaagtt taaactcatc cttagaattt attctgcctt cttctacata
    10081 ttcgtctatg tattcattta attcttcttt acttatgtac ttatccatat ctactagtaa
    10141 ccctaattta tccatttcat atgcagtatc tgagtatgct gcaaatatat ttggtacatc
    10201 ttcagaacca acttttttat ttgcagaatc taaaactttc tctgataaat caactacatt
    10261 accttgactg aatgcttcaa caataattcc tttttcagca cctacagtgt cattaaattc
    10321 cttaacaagc ttatcaaaag ccattttctg ctgtccatta taataatgcc aaatttgtat
    10381 tgatgtcgga ttttctgggt ctaacttata attatcttcc ttctttaaac tacatcctgt
    10441 aagtaataaa acaatcattg atgtgataat taattttgca aacttacacc tattcataaa
    10501 atctactcct tcaaattata aaatttataa tagcattaaa actgtaactt cactatttat
    10561 attaattata gaacgtttat tgatatattt aaacaggatt tacaacatat ttaaaagcaa
    10621 ttaagaaaca tactattaca aatatagaaa ataaagcttt tacataattt ttatttctaa
    10681 atgcaccaaa tgacaccaat ccttgttgaa tagctactgt aattactaaa agtatcatta
    10741 ttatattaag ttgaaccagt ttaaataagt aaagcaatga aaacactact agaagtacac
    10801 ataatacaat actagattta ttaataatat catcaatctt atctactttc ttagattgct
    10861 tattaatttt atttaatttt tctttattta aattctcaac ttttttatta tacctttctc
    10921 ttgttttatc tttatattta gtatttttat ttttatttcc catttatttt ccatcctcta
    10981 taaatttttt aattgagctt atacattatt taaatattat atatttaaga taccatatat
    11041 tcctacaaaa aggtaactta ctatgtatct atttttgatt ttcgcataaa aaagccatct
    11101 cctttagaat taattctgta ttgagacagc taatttaact atttatcctt tgaaacattt
    11161 tctacattat catatttact atcaactaaa actatatcaa ctcttctatt ttttgaccta
    11221 ccttcttcag atgaattttc tgctattggt ctaaactcac catatcctac agcagataat
    11281 ttatctggtg ctatacctgc attgtcaata agaagttgaa caacattggt tgctctcata
    11341 actgataaat cccaatttga cttaaactct gaatttttta tagacatatt gtctgtatgg
    11401 ccttctactc tcatgtaact attcatttca tttaacatct tacctatttg aataatctta
    11461 tctctagaat tatccttcac aatagctttc cctgtatcaa atagtattgt atctttaagg
    11521 cttattacta atcctctatc ttgaactctt aatgatactg aattagccat accattttcg
    11581 tttaaatact tctgaatttc ttcaccaatt tttttaaact tagcattttg taaagtatct
    11641 aaatcattac ttcctggttc aactactggt tctatcttgc tattaccacc ttcaataact
    11701 ccagaagaac caccaaaagc agaatttaga gattgagata attgtttata tttctccgca
    11761 tcaacattac tcatagaata cattattaca aagaaaatca taagtaatgt tatgaggtct
    11821 gaataagtta aaagccatcg ctcattgttt tcttcttttt catcttcatc atacaacata
    11881 aacattccat ccttttatat tattcagctc taccaccaat ttcttcaagt tcaattagtt
    11941 cttttgaatt taaaaatatt tttaattttt ctgtcattaa attagaatta tcaccctcaa
    12001 gtatggataa tattgcttct atcaccaaaa gtctttcact tacttcttgt tgatttatag
    12061 ccttaagttt tgatgctatt ggtaaccata ttaaattggc agaaccaaca ccatacaaag
    12121 tagccaagaa tgcaacagaa atttgaggtc ctaatgcatc tggattactt gaaagattac
    12181 ctaaaatatg tactaatccc ataactgtac ctattatccc cagtgtaggt gcatatccac
    12241 ctgctgaatc aaaaattgct attccttctt tatgtcgttt tgatgttatc tgtatctgtg
    12301 attccaatat atttcttata gtaactgcat ccactccatc taccataagt tcaagccctt
    12361 ttctaataaa agggtctaat tcatcattct cacttatttc tttgtcaata ctaagaagcc
    12421 cctctttcct agcctttgtt gcaatttctt tgaaatagat tatattttct atgttattag
    12481 gctttttatt tttaaaagct aataacatta tctttataga ctttttaaac acagggaatg
    12541 gagtcgaagc tccaacagct cctatagttc caccaaacac aacaagtgct gatgaaaata
    12601 caaataatgc tccaacatgt cccccatcaa taacaaaagc aactaccaaa gatgctatcc
    12661 ccactatgaa aaaaattaaa gttgtcatct attccctcct ttctttagtt atacatatct
    12721 aatcatttat gacattataa aaagactatc tttaataaaa atagccttta ataattggcg
    12781 gagaaggagg gattcgaacc ctcgcaccgg ttacccagcc taatccctta gcaggggatc
    12841 ctcttgagcc acttgagtac ttctccatat ctctatacta ataaattata taataaaatc
    12901 atattttagt caattgtttt ataaaatttg agttattttg aattattaaa catctatttt
    12961 tacttttttg cttaaatgta agttttaaat atggtaaaat tatcttgcat aacacttata
    13021 atattatttg ataaatatac atacattaac aaatatacct aaactctgac ttaattgtaa
    13081 gttatgccaa aactaaaata atactagtcc aaggaggata ttttatgtta ccacaggaaa
    13141 gatatgagaa gattttaagt attctcaaat cagataatat aattaaaata aatgacatag
    13201 ttcaaatgtt tgatatatca atagaaactg cacgaagaga tttagcccat atggagagta
    13261 tcggtctttt aaaaagagtt tatggtggtg ctataccagt tatagcaagt agcgtagaac
    13321 ttaactatgt taaaagaaaa aaaataaact caaacgataa aaaacagatt gctattaaat
    13381 gtagtgagtt tataaataca aatgatacta tttttatgga tactggaact actgtacttg
    13441 aagtttcaaa aaatttgaaa gataaaaaga atctaactgt aataaccaac tcaattttag
    13501 ttgcaaacga acttatgaat actaatataa aagtatatgt gcttgggggc tatcttagag
    13561 aaactgaagg ttctttttct ggtccgctta cactgtcagc tattgaacaa tttaatgttg
    13621 ataaagctat tttaggcgct ggaggtatca ctcttgaaaa tggaatctct gactatcact
    13681 tagaagaagc acttgttaga aaaaaaatga ttgaaaggtc tagaaaatct atagtagttg
    13741 tcgaacctcc taagtttggt ataaattcat ttgcatcagt agctcctata gaacatatag
    13801 atactattat tacctctgct agtatagacc ataatataat aatagagttt tctcaaaaag
    13861 gaataccttt agtcatagct gataattagc ttatatgatt gatttttagt tccttgatgt
    13921 aataaattaa aattaaggag ccaaataatt atttatttag ctccatttat gtctaaacta
    13981 tactttatat tttaattatg taactacttt cagttagctt atcattttta gttatttgat
    14041 tgccatttta tttaatttat aagttttagt tattcgatta ttttttaatt gtctgtcttt
    14101 ttaatttatt tttagttatt taaactccta ctttaaaata atttaaattt gacttaagta
    14161 ttgtttattc tacactgcat ctttcttcat tttaatactt tgttcttctt ccaaggttaa
    14221 atcttttgtc ataagtatag caagtgcaat tgccaatact cctccaacca attttccaat
    14281 tacaacaggt gtaatcatct ctggaactac tcctgctgta aatcctaaat ggtcccccaa
    14341 agctgctgta gcacaaacca gccaagcagt atttactact ttcccctttg gactcatatc
    14401 tttcatcatt ttatatactg gaatactatt ggctaaagta aaaactaatc ctgctgcact
    14461 agtggcatcc atacctaatt ttccacccac tattgtaagt ggtttattaa gaactttaac
    14521 taataaatgt aatattggaa aagtacctaa taacacgatt gcaatacttc caattacttg
    14581 aataccttct cttataggtg tcatgccttt tataagtact atacctgtta tttcttgaaa
    14641 tgctgctgct cccaaaccta tcgttatcaa tatagatata aattttccaa aaattaatga
    14701 cccatttatc ataagagtag aattaaattt taaacctact gccaatatta ctgccaatat
    14761 tatcactggt atcatattta taattaccat tgataagtta aatcctgcta ttaatcctcc
    14821 tataatacct ccaaagggta ttgttatcaa gcctatcagt aacccttttg caaagaatgg
    14881 tctgtcttca tattctataa gacctagtcc tactggaatt gaaaatacta atgtacatcc
    14941 aagcatggat gacacaacca atcctgcaag cattcctgcc tctttattgt gtgcaagttc
    15001 catagccaat ggataccctc ccatatcatt cgctaaaata gttgcaaaca tagatgggtc
    15061 agctccacag agattgaaga ttggtattat tataggacct agtacatctg aaattactgg
    15121 agctaaacaa actataccaa ccattcctaa agccaatgga cccattgaat taaacccttc
    15181 ttcaaactgc tcgccaagtc caaatttatt tccaattatt ctatctaacg ctcctaaaag
    15241 cactcccaca gccattatgt ataatataat tttatcaaag ggcattttta attcctccta
    15301 tacatgtaaa atactctatt gtaacttaat tagcacacac ctttttggta tcgttttccc
    15361 aattcatagc tctttttact gcttggttcc atccaagtaa tatttcttct cttttttgag
    15421 tagtcatatt tggaatatat tttttgctta ttgtaaattc acttctaatt tcatcaatac
    15481 tgttccagac accacaagaa agacctgcca tatatcctac tccaagtact gtcgtctcag
    15541 gattttctgc aatttcaatt ggtataccta atatgtcgga ttgaaattgc attgtaaagt
    15601 tatttttagt aattccacca tctactctca acatttttat ttttttccct gaggctaagt
    15661 tcatagaatc aacaacatct ttgacttgaa gtgcaacaga ctcaagagtc gctcttacta
    15721 tatgctcttt tttagtactt cctgttatcc caaccattgt acctctagca tattggtccc
    15781 aataaggtgc tgctatacct gcaaatgctg gtacaaaata tacacctcca gtacttgata
    15841 tggaatttgc catgtcatca gtttctgaat aattttttat aactccaatg ccatctctaa
    15901 gccattgtat tgcagcacct gctatataca ctccaccatc ataagcatat gtaactttat
    15961 ctcctatttt ccaagcaaca gttgtcaaaa taccattacc tgctatattt agttcctcac
    16021 ctatattcat atataggaaa catcctgtac cataagtatt tttaaccatt cctggttcaa
    16081 aacatccatt gccaaataaa gctgctggtt ggtctaccat tgaagcagta atttttatag
    16141 aaaccccaca aaatgcctcc ttatctgtag ttccaaaatc accactcgta ggcattatat
    16201 ctgctaaaat acttctaggt actcctataa tgtcaagcaa ctctctatcc caatccatat
    16261 ctcttatatt aaaaagcatt gttcttgctg ctgtagatac atcagttaaa aaagattttc
    16321 ctcctgtcat tttccatatt aaccaactat ctaatgttcc tgcaagtact tctccatttt
    16381 taattttttc ttttacacct tttacattat ctattaccca tttaattttt gtacctgaga
    16441 aatatgggtc tataattaac ccagtcttat ctattatctt attttctaaa cctggtatat
    16501 tctttaaatt ttccacataa tcataagttc tcctgcactg ccacacaata gcattgtaaa
    16561 gtggtttacc tgtattttta tcccataaca ttactgtttc accttgatta tctataccaa
    16621 ttccttttat atcactttca ttgattccat tttcaaaagc ctgttgtaat acattattac
    16681 tcacagtttt tgtattattc caaatttcta tagggtcatg ttcaacatat ccagattgtg
    16741 gatatatttg agtatgttcc atataatcgc taaaaacaaa ctctgatttt tcgtttatca
    16801 atactgctct tgttcctgtt gttccttggt caattgctaa tacatatttc actttaaatg
    16861 tcctccttta ttttcagaat taataaattc ttttttattt tttgaatata taatctattc
    16921 aagttattta tattttatat catcatgtgg atttatgcaa tattcattac aatatttttt
    16981 gttattttta attatagtga ttgaaaaagt atttttattg aaatattgtt gcaatattta
    17041 ttattttatt ctataatcta gttaaaataa cacataatat tttaatattt acacataatc
    17101 agtcattcaa aaatttaaat ttaacttttt aaggaggctt tacaatgaaa aaaacatatc
    17161 tattcagtcc tggaccagta atggttacag ataaagtcag aaattcactt cttcactatg
    17221 atatatgcca tagaggaaat gagtttatgg atttattcaa agatacacaa caaaaaatca
    17281 ataaactata taatgcaact tctaattact actcattaat tgtatctggc tctggaacat
    17341 ctgtaaatga atgtgtacta tcttctatct ttgacaaaga agatgcagtt ctgctagtta
    17401 gtaatggtgt ttttggagaa aggctagaag aaatattatc tacttataat gtaaaaacat
    17461 ataaacctaa ttttgaatgg gcagaatacc cggacctaga cattttagaa aaatacataa
    17521 ttgaaaactc tgatataaaa gtaattgcta tggtgttcca tgaaactagt tcaggaatga
    17581 taaatccagt ccctgaacta ggaaaacttg ctaagaagta taacaaaata ttttttgtag
    17641 atgcaataag tgcctcagca ggtgaatata ttgatgtgga agagtttaat atagatataa
    17701 taacaggtgt tggtggcaaa gctcttggag catttccagg ttctgcatac ctttgtgcta
    17761 aagagagtat tttacaaaat attaaagaaa atcaatgtaa gaatgtgtat cttaatcttt
    17821 ataaacacta taaagttgct aaatcatctt ctcaaacacc aaatacacct aatgttactt
    17881 taatatttgc ccttaatgaa gctttaactg agtttttgga agatgacagt aaaatagaaa
    17941 gatacaaaga atgttctgct attttaaggt atggtatgga agaattgggt ctaacatttt
    18001 tacttccaga taagtatatg tctaatacag taacatctgt atttctacct aaagaaatca
    18061 atgtaaatga gtttatatta gggctagaaa gggaaagtgg ctatgttgtc tatccaggaa
    18121 aaggcaaatt cctagatgct aacatattcc aagtagcaaa tatgggagaa atatatccag
    18181 aagattgtta taaattctta gatatcttaa aatcaaaatt ggagataaaa aaagttatat
    18241 aacagtttgt ttgtaaaatc ctacaatata tcagtataaa ttaaatagac tgattttttt
    18301 aaattttcct ataat
//
LOCUS       DAYASV010000074        18306 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYASV010000074 DAYASV010000000
VERSION     DAYASV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.074"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            459..2129
                     /gene="pdcB"
                     /locus_tag="ACGI68_002401"
     CDS             459..2129
                     /gene="pdcB"
                     /locus_tag="ACGI68_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase PdcB"
                     /protein_id="HFH0138835.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHRMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESNKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2251..2985)
                     /gene="pflA"
                     /locus_tag="ACGI68_002402"
     CDS             complement(2251..2985)
                     /gene="pflA"
                     /locus_tag="ACGI68_002402"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFH0138836.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNIDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3145..5376)
                     /gene="pflB"
                     /locus_tag="ACGI68_002403"
     CDS             complement(3145..5376)
                     /gene="pflB"
                     /locus_tag="ACGI68_002403"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFH0138837.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5735..6730
                     /locus_tag="ACGI68_002404"
     CDS             5735..6730
                     /locus_tag="ACGI68_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFH0138838.1"
                     /translation="MFITVVLFLVGFLLITKGADIFINCTVEIGKKTNISEIILGATI
                     VSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICNTGLALGLVVFISPFNVDKKMFF
                     SKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIFYMVNNYRSVVGKSDTKNRNIKN
                     NTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMMIIGSQILIESGVIIASFLNIPQ
                     GIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNILGANILNIVSVIAVSAIPNNIP
                     ILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGILMLFTYFLYISILYFMYII"
     gene            6821..8434
                     /locus_tag="ACGI68_002405"
     CDS             6821..8434
                     /locus_tag="ACGI68_002405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0138839.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            8709..10694
                     /locus_tag="ACGI68_002406"
     CDS             8709..10694
                     /locus_tag="ACGI68_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138840.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            10699..11133
                     /locus_tag="ACGI68_002407"
     CDS             10699..11133
                     /locus_tag="ACGI68_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFH0138841.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKNLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11162..11722
                     /locus_tag="ACGI68_002408"
     CDS             11162..11722
                     /locus_tag="ACGI68_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFH0138842.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            11786..12286
                     /locus_tag="ACGI68_002409"
                     /pseudo
     CDS             11786..12286
                     /locus_tag="ACGI68_002409"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
     gene            12326..12745
                     /locus_tag="ACGI68_002410"
     CDS             12326..12745
                     /locus_tag="ACGI68_002410"
                     /inference="COORDINATES: protein motif:HMM:NF019286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbitol transporter subunit IIB"
                     /protein_id="HFH0138843.1"
                     /translation="MAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFP
                     LLSPFLGPGAVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAE
                     AEPDTVQVGVPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            12786..13151
                     /locus_tag="ACGI68_002411"
     CDS             12786..13151
                     /locus_tag="ACGI68_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFH0138844.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13252..14025
                     /gene="srlD"
                     /locus_tag="ACGI68_002412"
     CDS             13252..14025
                     /gene="srlD"
                     /locus_tag="ACGI68_002412"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFH0138845.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14374..16518
                     /locus_tag="ACGI68_002413"
     CDS             14374..16518
                     /locus_tag="ACGI68_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HFH0138846.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELD
                     ITSNKTDYISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKV
                     CFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFF
                     MELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKF
                     ETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMD
                     KKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKK
                     HGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            16606..16941
                     /gene="spoIIAA"
                     /locus_tag="ACGI68_002414"
     CDS             16606..16941
                     /gene="spoIIAA"
                     /locus_tag="ACGI68_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HFH0138847.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            16955..17383
                     /gene="spoIIAB"
                     /locus_tag="ACGI68_002415"
     CDS             16955..17383
                     /gene="spoIIAB"
                     /locus_tag="ACGI68_002415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HFH0138848.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17406..18170
                     /gene="sigF"
                     /locus_tag="ACGI68_002416"
     CDS             17406..18170
                     /gene="sigF"
                     /locus_tag="ACGI68_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HFH0138849.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAIKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
BASE COUNT         7117 a         2074 c         3172 g         5943 t
ORIGIN      
        1 tataatccct attaaaaaat ttatagatga tgaagaaata tcaaaatctt atattatagg
       61 aagagacact tctttgccaa aatctataga aaagcaatac caaatccaaa gagaataggc
      121 gaagaaaaaa ataaaataaa ttcaagagta atagaaaagt tttatagaat aaagaactga
      181 taactcttta tataacgaaa aatagaattt taagagaaaa tgatttaata aatgatttaa
      241 catggtgttt tagcaggtaa aaataaatca ccagtaatga ttatagaaaa attactttat
      301 gaatctcaaa aaacttagtt aagagtaaga gatttgataa gataactaaa gttggggaaa
      361 tgtgtttgat gagacaaaaa attagtaaaa tagtataatc taaataagga tattttgttg
      421 attaaactac actaatagta atattgtagg gggaaaagat gagagaagaa tcgaactctt
      481 taagtttaga ccatatagaa attttctttg aacttttatc tcaaagtaca gaagattata
      541 tatttttttg ggatataaat aggaataaat ttaaaatttc ttcagcaatt tttgatgaat
      601 ttaacttaag taaagagata gaatctgatt tggtcaattg ttggagtaaa attgtttatc
      661 cggatgatgt tcaaatatgg aaagatgata tacaagaatt actacatgga aaaaaaggtg
      721 agcacaattt agaatatagg ttgattaata aatataaaga aatagtatgg atttcttgta
      781 gaggtaaagt ttatgtgtcg gatgacccaa aaactatatt tctagtcggg agaataaaga
      841 atattggtga aaaaaataaa tttgattcta tcacaggaac gtggaataga gaacaatttg
      901 aacatagaat gaattatctt ataaaagaga aaatttataa aaatggtgct atgtttatta
      961 tggatattga taattttaaa aatataaatg aaaaatatgg acattcttat ggtgacaaag
     1021 tattgcgtgc aattgctact gaagtattag aatatcttcc aaaagatgta agattgtata
     1081 ggttagatgg agatgaattt gcattttttt accctatgtg tactaaagaa actatagaga
     1141 aaatatatga aaagatacag atgtatacaa atactcagca tgaaattgaa tcaaataagt
     1201 actattgcac agtgactgca ggcgttgcta tgtatccaga agatggagat aattatttag
     1261 atttatttaa acatgctgat attgcattgg atatagcaaa aattagtggt aaaaatagaa
     1321 ttaagttttt ttcacaagag ctctatgaaa ataaactaaa ggtaatttca atgcaacaaa
     1381 aactaagaga gtgcgttgaa aataatttta atgattttga gttgtttttt caaccccagg
     1441 taaatgctgt tacaaaagag gtaatagggg cagaggtact tttaagatgg cattcgagta
     1501 cttatggaga agtatctcct gttgaattta taccaatact agaacagagt aatttaatta
     1561 taccagttgg gaagtggatt ataaaagaag ctgtaaaaca gtgtaaagaa tggcataaaa
     1621 taaatccaga ctttaaaata tctgtaaatg tatcgtatat tcagttaaag gaagattttt
     1681 ttagagattt tatagtagaa tgtttagtag agtatcaatt aagacctgaa tttttaattt
     1741 tggaattaac agagaattgt tggataccag atataaattt actaaatgat aaatttatta
     1801 gtttaaaggg tataggagta tatattgcaa tagatgactt tggtacagga tattcatctt
     1861 taaattacct taaagaactg tctgttaata taataaaaat tgaaagaagt tttgttaaga
     1921 atattacata taatagttat gaatatactt ttcttgaata tattattaaa ttagctcata
     1981 ttataaattt aaaagtttgt gttgagggaa tagagtctta tgaagagtat gatatagtaa
     2041 aaagcttagg tgtagatatt attcagggtt ttttatttgg aagacctgta agtgcctctg
     2101 aattttataa atttcagcta tcaaagtgat gtatataaaa tgagatatat agtatataaa
     2161 aatagggcaa agtggaagtt ttgaagttta ttaaaaattt acactttacc ctattttgtt
     2221 atgtcttcta taaaggatta atatactaca ctaactatta tatactttca ctccaaattt
     2281 agagattatt tcactagctt tttggacatc ttccttagtg gcatcaggaa catcttttag
     2341 ttcataatcg atacctaaac tttcccattt gtgtattccc atagaatggt atggtagcaa
     2401 ttcaaatctt tctacatttt tcaatgtagc tacaaattga cctagttttt ctaaattatc
     2461 tatatcatca gtaatgccag gaactaaaac atgtcttatc catacaggaa tatttcttga
     2521 ttctaagtgt ctagcaagtt ttaatgcctt atccatacca acaccagtca aatcaataga
     2581 tttttcctca accatatgtt ttaaatcaag taaaaccaaa tcaatattat ctaaaactgg
     2641 gtctatagtt tctatatcta caaatccaga tgtatccaag caagtgtgta tatcattttc
     2701 ttttgctttt ttaaataatt cacttaaaaa ttctggttga agagtggatt caccaccaga
     2761 ggcagttata cctccaccag aaaacttcat aaaagaagta tacttaagag catccgttat
     2821 tatttcatca actgtgtatt cttttcctga tttaatatcc catgtatccc tgttatgaca
     2881 gtatttgcat ctaagaggac acccctgaaa aaataatata tatcttattc ctggtccatc
     2941 tacagtacca aacgtttcta tagaatgtat ctttccctta atcatagagt tcacctcaca
     3001 atatatttta ataaccttat caatagttat aatatttctc taaaaaaggc catcttatac
     3061 cagaaataat tcaaatataa gatagccttt tatttttatt ttttacacag tagtaatctt
     3121 agttaaagta taaaaattta aagactaagc cattttaccg tggaatgttc tgtttataac
     3181 atctaattgt tgctctttag ttaattttat gaagttaact gcatatccag atactcttat
     3241 agttaattgt ggatattctt ctggatgttc catagcatct tctaaagtag ctctatcaaa
     3301 tacatttaca tttaagtgat gagcattttg agcgaagtat ccatccatca ttgcagaaag
     3361 attgtttatt ctttcagtca tatcttttcc taaagcacca ggtactatag agaaagtatt
     3421 agatatacca tcttgagcat gttcatatgg taatttagca actgaagata atgaagctaa
     3481 agcaccatta gtatctctac catgcattgg gttagcacca ggagcaaatg gagcaccagc
     3541 tcttctacca tctggtgtat taccagtttt cttaccataa acaacatttg aagttattgt
     3601 aagtatagat tgagttggat aagaatttct ataagtttta ttctttctta ttttattcat
     3661 aaagctttca actaactcta cagctatact atcaactcta tcatcattat ttccgtattt
     3721 tggataatct ccttctattt caaaatcaac agctattcct tgttcatttc taatagtttt
     3781 aaccttagca tgttttatag cagataatga gtctgcacaa actgataacc cagccatacc
     3841 acaagccata gttctaaata tatctctatc atgtaatgcc atttctaatg cttcataaga
     3901 atatttatca tgcatataat gtattacatt taaagtattt acatataatg ttgctaacca
     3961 atcagtgaat ggttcaaatt tactcataac ttcatcataa tctaagtact cagaagttat
     4021 aggttcaaat cttggtccaa cttgaacacc agatttctca tctacaccac catttatagc
     4081 gtataataaa gttttagcta agttaactct agctccaaag aactgcattt gtttaccaat
     4141 tctcattgca gatacacaac aagctatacc atagtcatct ccccagtatg ctctcattaa
     4201 atcatcattt tcatactgta ctgaacttgt atctatagat actttagaac agaaatcttt
     4261 gaatccttga ggtaatttag ttgaccatag aactgttaag tttggttctg gagaaggtcc
     4321 taatgtatat agagtattta acattctaaa tgagttttta gtaactaatg ttctaccatc
     4381 tattcccata cctcctatac actcagttac ccaagttggg tctccagaga ataggttatt
     4441 gtaatcagga gttcttagga acttaaccat tcttaatttc ataacaaaat ggtccatata
     4501 ctcttgtaat tcttcttctg ttacgattcc agcttttaaa tctctttcaa agtatatatc
     4561 taagaaagta gatgttctac ctaaagacat tgcagcacca ttttggtctt taacagcacc
     4621 taaataacca aagtataacc attgtacagc ttcttttgag ttagttgctg gttttgatat
     4681 atcaaatcca taagattcag ccattttttt aagttcattt aaagctgata tttgctcagt
     4741 tatttcttct cttaatctga taacttcctc atccatgcaa gaaacttcta atgatttctt
     4801 ttgctcattt ttatcttcta ttaaagcatc aacaccgtat agagcaactc ttctgtagtc
     4861 accaattatt ctacctctac cataagcatc tggaagacct gttattatac cagattttct
     4921 agcagctctc atttctggag tataaacatc aaaaactcct tggttgtgtg tttttctgta
     4981 tttagtaaat atatctttta tttctgggtc taattcatat ccaaaagctt cacatgaatt
     5041 ttcaaccatc tttataccac catatggcat gacagctctt tttaaagggg catccgtttg
     5101 taacccaact attgtttcta attctttatc taaatatcct ggagcatatg cagctattcc
     5161 agaaacagtt tttgtatcaa catctaaagt accaccattt tctctttctt ttttaaatag
     5221 gaccattgct tcgtcccata attttttagt attttcagtg gcaccagcta agaaagagtc
     5281 atttccctca tatggagaat agtttaattg tataaactct ctaacattta tttctttagt
     5341 ccatcttcct gttttaaatc cttgccatgc attcattaag tatttcctcc ttagatttaa
     5401 atgataaata atactattaa aattaatttt tatatactta atttatttta tatatttttt
     5461 taaaaatata ttttctaata taataatagc atattattcg tatcctgtat acataaaaaa
     5521 taaaaaattg tcgctttaaa gtataaaaca gattatatgt taataatgtg gtgaaattat
     5581 acccatatat aaataaaata aaacaaataa atagctaatt aaggagaaaa tattttttat
     5641 tttttgttta ggataaataa attttattat ggaaaaaaga aaaagcatac atcttcacaa
     5701 actgaatatt tatttaatag ggggaggtga tagattgttt atcacagtgg tattattttt
     5761 agtaggtttc ctattaatta ctaaaggcgc agatatcttt ataaattgta ctgtagagat
     5821 aggtaaaaaa acgaatatat cagaaattat acttggagct actatagtta gttttgcaac
     5881 aacattacct gagtttacag tgtctctact tgcttctatt gatggtcata ctactatgag
     5941 tttaggaaat gcagttggtt ctataatatg taatacaggg ttagcattag ggttggttgt
     6001 ttttataagt ccatttaatg tagataaaaa aatgtttttt tctaagtctt tactattaat
     6061 agtatccgtt atagttttaa ttttattgag tttagatgga gttataacaa gaggtgattc
     6121 attattactt ataataatat taatctttta tatggttaat aattatagaa gtgtcgttgg
     6181 aaaatctgat actaaaaata gaaatattaa aaataacact attaaagaca tatctactaa
     6241 aaaagggaaa aattatagag gtttttccgt attagaaatt gtaaaaatac tattactttt
     6301 tgctacagga ttgataatga tgataatagg ttcacaaatc cttatagaga gtggagtaat
     6361 aatagctagt tttttaaata tacctcaagg tatagtaagt ttaactataa tagcacttgg
     6421 aacttctctg ccagagatag tttcgtcgat tactgctata agaaaaaatc atcatgaaat
     6481 atcagtagga aatattttgg gtgcaaacat attgaatata gtatcagtaa tagctgtatc
     6541 agcaatacca aataacatac caatactatc tcaaaataga caattggata taccattcat
     6601 gatattatta ttattaatag taataatacc aacacttaaa tctaataaac ttagtagaat
     6661 acaaggtata ttgatgttat tcacatattt tctgtacata tcaattttgt atttcatgta
     6721 tattatttaa ctatactgtt taaaatatta attgcagaat atcctaaaaa agtatataat
     6781 ggataggtta agattaattg ataacggaga ggaataatat atgaatataa caaagtttga
     6841 agatttacca ataagtgagg gaataaaaaa agctatagct gaaatggggt ttgaagaacc
     6901 atctcctata caagcacagt ctatacctgc gattttgtca ggtaaagacg ttataggaca
     6961 agctcaaact ggtacgggaa aaacagctgc ttttagtatc cctatactag aaacaataga
     7021 cccaaataat agaagtttac aagctgtagt gctttgtcca acaagagaac tggcaataca
     7081 agtatcaaca gaaataagaa agttagctaa gtattcgcac ggaataaaga cattacctat
     7141 atatggtggt cagccaatag atagacaaat aaaatctcta aagagtggtg tgcaagttgt
     7201 aataggtaca ccaggacgta caatagacca tatcaatcgt aaaacattaa agatggataa
     7261 tgtaaaaatg attatattag atgaagcaga tgaaatgcta gatatgggat ttagagaaga
     7321 tatagaaatg attttaagta agattccaga agaaagacaa acaactttct tttcagctac
     7381 tatgccaaga ggaattttgg aattaactaa gagatatcaa aaagacccag agcatataaa
     7441 agttgttaga aaagaactta cagtttcaaa tacaaagcaa tactatatag aaactaggtc
     7501 atcaaataaa ttagaagtat tatgtagatt agtagatgta tatgacccta aactatctgt
     7561 tgttttctgt aatactaaga gaaaagcaga cgaattggta ggagatttac aagcaagagg
     7621 atattttgca gatgcattac atggtgactt aaaacagact caaagagata tagtaatgga
     7681 taagtttaga aatggtacaa tagatatact tgttgctact gatgttgcag ctagaggtat
     7741 agatgttgat gatgttgaat gtgtattcaa ctatgactta ccacaagatg aagaatatta
     7801 cgttcataga attggtagaa ctggtcgtgc tggtagagaa ggtatgtcat ttacttttgt
     7861 atttggaaaa gaaatgagaa agatgaaaga tatagaaaga tatactaaat ctaaactaat
     7921 aaagcataat ataccaacaa taactgatgt tgaagagaaa aaagtaggta ctttctttgc
     7981 acaagttaaa caaactatag aagaaggtca cttaactaaa caattacaat ggttagaagg
     8041 tttctgtaat gatgaagatt atgcaatggt tgatatagct gctgcacttg taaaactatc
     8101 tctaggtgaa gaaatgaaag aagaaataat agaagaaaaa cctagaagag agcgtggaga
     8161 tagaaaaggt ggtactggag ctaaagatgg tatgattaga ttatttatca atattggtag
     8221 aaatcaaaga gtacaagcaa aagatatagt tggtgccata gctggagaag ttggaatacc
     8281 aggtaaagta gtaggtacta tagatatata tgataagtat acttttgttg aaatacctaa
     8341 aaaagatgca aaaacagtaa ttgaaaagat gaaagatatt aagataaaag gtaataagat
     8401 taatatagaa aaagcaaata agaagaaaaa ataacgtata gtatacaaaa ctataattag
     8461 aaaatatata aaataatata ttggatattt aaaaagcctg atgtgttttc aaaacagata
     8521 gtagataatc tgtaagagtt aacagcataa ggcttttctt gttttatatg atgttataat
     8581 tttccagatt gttgtgaaaa atgtatatta gttccttttt ttcaaatctg atttgaaaaa
     8641 gagaatatta attatctata tagtttagat tataataaaa aatgtaagat aacaaagggg
     8701 agaaaaagat gtcaatgact tatatggacc ctagggtttt tttgatttta gaaatggtgt
     8761 tagaagaaga cacaaatact ctagaacaat tttcaaatgt tttgggtgtt agtactagga
     8821 ctatcagaaa ctatataaac cagattaatc gagaattaaa aggtggtatt gctaaaatag
     8881 ttaaaaatga aaaaggtaca tatagtataa gaattgagga tgaaagtaaa ttaattgaga
     8941 ttgtaaattt taatagagga aagctatcaa attttataaa tttaaattca cctgatgaaa
     9001 gaataaatta tgttcttgat attttaacta tgaccaacaa tccaattacc atagatgatt
     9061 tggctgaaga gatatgtata ggaagaacaa cattaataaa ggatttaaaa aaagcagaca
     9121 gaattttagc tgaatatgat ttagaattaa aaaggaagcc aaatataggt atgattttag
     9181 ttggtgaaga aatggatata agattattaa tccttgatag attgtatgaa aattatattg
     9241 atgtcttgga aaacgttaat cagataaatt tagttaaaga ttatgatatt gaatgtctta
     9301 gagatgaatt aaaaaagtta tttaaaaaag aagaactcta cataacagaa caggtcttga
     9361 gagatgttga aagatatata atcatgtctg tcttaagaaa tctaaatggg tacaaatttg
     9421 aaaagataga taagagattt gaagtgataa gatgttcaga tgaatatact ttaggattaa
     9481 agctcaaagc tttattggaa aagatattta atatagtttt aaatgaaaaa gaaaccatat
     9541 ttttgacaat gccattaata ggtagaaaag ctcctagtac aaaattagca cttagcagta
     9601 taaagataaa tgatagcgta aaggaagttg tagatgaagt aactagtttt ttattagaga
     9661 cctcagggat tgattttaaa gaagataaaa agcttataga aaatttggaa tatcacttgt
     9721 attttgcact taatagaatg agattcaaca taagggtaaa aaatcctctt ttacaagaaa
     9781 taaaggaaag atatacattt ccatatagtg ttgcaaagat agctgcaatg ataattgagg
     9841 aaaagtttga cttgaaaata tgtgaccacg aaataggata tatagcatta cattttggaa
     9901 gttattttga aaaaaattca agaaaagttc taaatgtaaa tagggtagct gttgtttgtg
     9961 gaacaggtct tgggactgca caacttatta gaataaagat aggaaaaata cttggagaaa
    10021 gtgtagaagt aaatactttt tctgatatag aagctaaagc taatttagat ttattaaagt
    10081 attatgattt agtatttaca acagtagatt tcaaaacaga cttagatgtt ccaattttta
    10141 gagtaaatgc gttatttaat gaagaaagta taaagaaaga gatagaaaat gcaatacttt
    10201 tgaaaaatag tataaatctt aagtctataa aaaagattga aaaaaatatc ccttttatag
    10261 gtaaattaat tgaagaggat aagttcttta tattgaataa gagtacgttt atggaaaatt
    10321 tagaagatat gttagaaaaa ttagttcata aaggaattat tgatatagag ttcaaaaaaa
    10381 aggtattaaa aagagaaaag aaatcttcaa cagcattaga taattatata gcattgcctc
    10441 actcagttaa tactaatggg gaaaatcttt atctggcatt tggaatatta gaaaaaccag
    10501 tgatttggga tacaaaggaa attagaataa taatattgat gataattcca gataagaatg
    10561 tagatagtac agacctgata ataaaaagtt atgaagaagt acttgaagta gggagagata
    10621 aaaagatggt agaagaactt gctaaggcaa aatcatttga tgaattttat aaaataatta
    10681 ccaagaggag gtaataatat ggacccagta atttttatta tagcatcact tattacagtg
    10741 tatttattaa atatttttct tggatactta cagcttaaag attttaataa aaattatatt
    10801 gaactaaaaa gaaaaggaag agtggcaatt ggcagaaaaa aaggaagaat ttcatcgggt
    10861 acaattgtgt tgatacttat aaatgatgat ggtgtgattg tcgagactag aaaaatgcaa
    10921 ggtgttacag tattggcaag agtaaagtca tttgaaggtt tggtaggaaa gaacttagag
    10981 tatatagaaa aatcagattt aactgtttac aataaactac ttaaaaaagc aatactagat
    11041 gcagtcaaac agtacataac atttgagaaa aataaatgtg aagatacaaa taatctgagt
    11101 actgatgaag agtgtaggat ggaagtagtt taaaaaaata tatttataag gaggagttaa
    11161 tatgacgcaa ttttttcaag tattagcaag tggtgcagat agttttatga atttatttag
    11221 ggcaggtggg caacaatttg taggttttgt aacagatata gtaccacttt tagtagcatt
    11281 attagttaca atgaatgcaa ttatcaactt tattggtaca gatagagtcg agagacttgc
    11341 aaaaaaatgt tctgcaaata tatttactag atatttgata ttaccagtcc ttggtacatt
    11401 tgtatttgca aacccaatga cactatcatt aggaagattc ttgccagaaa agtttaaacc
    11461 tagttacttt gcagcagcaa gcttttcatg ccatacaatg aatggattat tcccacatat
    11521 caatccaggt gaactattta tatatctagg aattgcaaat ggtataacaa ctttgggatt
    11581 ctcaacagca gatttagcag ttagatattt gttagtgggt atagttgcaa actttattaa
    11641 aggtgtagtt actgactata caacaaaatt tgtagagaaa caacaaggaa tagcattaaa
    11701 ttcagatata aagattggat aattacttta aggtataaat aaatttttca aataaaaaga
    11761 tagtgtatag aggaggataa attatatggc atttaacaaa gttaaaatag taaaaggttc
    11821 tggaggatgg ggtggaccat tgataataga accaacagaa aagaagaata aggtagtata
    11881 tgtgactggg ggagcaaagc cagaaactgc agtaaaaata gctgaactta caggatgtga
    11941 gttaatagat ggattttcac agggtgttaa agatgatgaa atcgcatgtg taataataaa
    12001 ttgtggtgga acattgagat gtggtatata tcctcaaaag aaaataccga ctataaatat
    12061 aatgaaaaca ggaaaaagtg gaccattagc aatgtttata aaagaagata tatatgtttc
    12121 aggggtaaaa ccaacaaatg tagagcagtt ataattgagg tagtaggagg gaagaaatat
    12181 ggcttatgat actaaaaaga agataagtga gcagagtcaa ggaattgttg caaaaatagg
    12241 tatagcacta ggaaatgtga caagtactct ttttcaagct ggtagggata ctatagatac
    12301 attaatacat actatactgc cttttatggc tttcgttgca gtcctaatag gtataataaa
    12361 tcaatcaggt ataggaaatt gggtagccaa tttacttact ccattaatcg gaaatgttgg
    12421 aggtttagtt gtcttatcaa taatatgttc atttccgttg ttgtctccgt ttcttggacc
    12481 aggagctgtt atagctcaga tagtgggagt attggttggt acagaaatag gaaaaggaaa
    12541 tattccacca agtttagcat taccagcatt gtttgcaata aactcacaag cagcttgtga
    12601 ctttatacca gtaggtcttg gtcttgcaga agctgaacct gatacagttc aagtaggagt
    12661 tccgtcagtt ttatattcta gatttttaac aggtgcacca actgttctat tagcatggtt
    12721 agcaagcttt gggttatata aatagaagaa tactttataa ataattagag agaggttcag
    12781 ttgatatgga attaatatac agtactcaaa taaataaaat aggagataat gcaaatgaat
    12841 ttttacaaca taatatgttt gtaactttca aagataatgc tcctcaagag ttagaaaatt
    12901 actgttatat acattctgaa aataatttag taaaagaaat acttacggat gatgtgctat
    12961 caataaataa tgttgattac aaaattactt ctgttggtga gttagtaaac caaaatcttt
    13021 cagaattagg tcatataaca tttaagttta caggtgaaaa agatggagca attggaggaa
    13081 ccttatatct tgaagaaaaa gaaatagcac ctattggcgt aggtactata ctaaaaataa
    13141 ttagaaaata aaatttaaaa cgaatctaat catattataa gtaaattata gataaaagtc
    13201 aatttaacta atcaagttaa cttttaatgt gataaaaaag gggaaatgaa aatgagaaaa
    13261 gtggctttag tcgttggagg aggaagaagt ttaggtgaat atttatctaa acatttagct
    13321 aaagtaggtt atgatgttgc tattgcagat ttaaatgaag acaacgcaaa aaaagtagct
    13381 gaagaaataa aaactgaatt tggttgtaaa tctgtaggat atagttgtaa tgccatagat
    13441 gaaagacagg ttataagtac tgtcaaagct attgaaaatg attttgatag aatagattta
    13501 ttagtgtaca atgcaggagt ggctgtaagt aaaaaaataa ctgattttgg atacaaagat
    13561 tttaaattta ttgtagacgt aaatttaaat gggtactttc tatttgcaag agaagtatct
    13621 aaagtcatga tagcaaaagg tattgagggt agaataatac agataaattc aaaatcaggt
    13681 agagtgggaa gcaagcataa ttcaggatat tcagcaggta aatttgctgg agtaggactt
    13741 actcaaagtt tagcgctaga tttggcagaa cataagataa ctgtaaatgc actgatgctc
    13801 ggtaatttac ttgattctga tatgtttgaa agtttaattc ctcaatatgc agtaaaattg
    13861 ggaatacctg aatcagaagt caagcaagtt tatatagata aagtaccttt aaaaagaggg
    13921 tgtagattta tagatgttgc aaatatgctt acattttatg cttcaaagga agctgaatac
    13981 tgcactggtc aatctataaa tattactggt ggacaagtta tgtaaggttt ttatggagtg
    14041 tctcaagatg aactctttag ttcatgaggc acttttttgt ataaaattaa atatattttc
    14101 agcatttaaa caatgaaaaa gaggtttatc tttattttga gatagcctca tttttgtaat
    14161 taaaaaacat agtgtttaag taaacatata agattgttag ttttttaaaa gagaattttt
    14221 taataatatt atatttatga agtattttta aacttatttt gataaaatat agctatgttt
    14281 tatatactgt agttttgaac atagaaagtt taataaatta tagtatatat gttaattttt
    14341 aataaggtaa gaaagtagaa agaaggatat tttatggata taataaaaaa attaagtcat
    14401 gggtgtacat tagattgtca tgactgttgt aaattcaatg tttatactaa aggaaataat
    14461 gtagtaaaaa ttgaaggaga taaaaatcat ccttatacaa aaggttttat atgcaaaaaa
    14521 gggatggccc atttagatag attaaatcat aaagatagaa taaagactcc tatgcttaaa
    14581 gttgatggag tatggaaaga gatttctttt gataaagcta tagaaataat ggcagaaaag
    14641 cttacatact ataaagaaaa gtatacatca aagtctgtca tgcattatga ccaatatgga
    14701 agtgggtctg tgctaaaata cataggagat atatttttta atttttatgg aggtgttagc
    14761 agacataaag gtggtccctg ttggagtgca gggatgcatg ctcaaaaata tgattttgga
    14821 gtagctaaga gtcatgctat agaagatatg ttaaatagta aaagtatatt tgtgtgggga
    14881 aaaaatccag catatactac aattcatact atgcaaataa ttaaaaaagc taaagaaaaa
    14941 gggataaaga tagtagtgat tgaccccata tatacaaaaa cagctcaaat agcagataag
    15001 tatgtacaag taaatcctgg aacagatgga gctttagcta tagctatggc aaaaattata
    15061 gtagaagata aattatatga tgaagaatat ataaactcgt atgttattgg ttttgaagaa
    15121 tataaaaaat atttatcttc attggaatta agttttttaa ttgatgaatg tggtgttaaa
    15181 gagaatgata taagagagtt agtggattta tatactaaca aatattcaag tataaatgtt
    15241 ggatatgggc ttcaaaaata taaaaatggt ggaaacacaa taagagcaat agatgcttta
    15301 ggtgctataa ctggtcaaat aggatttagt ggtggagggg taaactatgc taataaagtt
    15361 tatccgagtg ttataaattc tgacccttac aatagccaat catatggtga agatagggag
    15421 ttttatgtaa gtaatatcag taaatttata gaagaatctt taaaaaatac atcaaataaa
    15481 gtaaattatg catcagatga acttgatatg acatcaaata aagtaaatta tgtatcagat
    15541 gaacttgata taacatcaaa taaaacagat tatatatcaa atgaattgta taatttatct
    15601 aataagtcaa tcaaaggtaa tattcctata aagatggctg taataactaa aagtaatatg
    15661 ctgaatcaac tgccagattt agtagaattg gagagggtgt tttcaaaaat tgaatttaaa
    15721 gtatgttttg atatgtttat aactgataca gctactttat gtgacatgtt tataccgtgt
    15781 acaaatacac tagagagtga agatataatt tatagctcaa tgaccaatcc atatataacc
    15841 tataatgaaa gagctgtaaa gccagcacat aaattgatgg atgaatatta cttctttatg
    15901 gagctagcta aaaagatggg attaaatgat tatccttttg tagaaaagag aacttattta
    15961 gaaaaagtga ttgaaccact taagcgtttt gacaaaaatc tggatataga aaaacttaaa
    16021 aacaattact ttactataca taaccctgtt gcttgggaag ataagaagtt tgagactcca
    16081 tctggaaaat acgagcttta ttcagaaagt ataaaaaact tgggaataag tccaacacct
    16141 gtatatataa gcaataaata taaagaaata gaagataaaa atatttcttt tagattatta
    16201 actaatcatc atgcagatac tttatttagt cagcatttta tggacaaaaa atctatagct
    16261 caagcatata taaatcggag aatggctaaa aaagtaggta ttgaggacaa agatattgtg
    16321 atattgaggt caaaaaaagc aaaaataaat gtacaaataa atatagatga tggagtaggt
    16381 aattatatag taaaaatgta tgttggttgg tggaagaaac atggaaatcc taatagttta
    16441 acagacactg gtatatcaga ttttgggggt caggtaacat ataatgaaag tatggtagaa
    16501 ataatacgac aaaactagtt aaatttcatt gaaggaataa aaatataatt atagaattga
    16561 ttaaaagtta ctatttttac aaaactattg aaggagggat ttttgatggt aaattattcc
    16621 ttagaacata agaatctata catagaattt atgtgctcag aactagacca ccatgtagcc
    16681 aatgaaataa gggaagaaat agataactta ttaagtgtaa atcaagtaaa aaatgttgta
    16741 tttaattttg aaaatattaa ttttatggat tcttcaggta taggcgtcat cataggtaga
    16801 tataaaaaga tttctaatga agggggacga gtttcagtaa ttaatataag ttcacgagtt
    16861 aagaagattt ttgatttatc tgggttaaat aaaattattg gcatttatga tacgtatgaa
    16921 gaagctctaa gttctttgta gggaggagta tgtgatgaat aatattatgg aagttaaatt
    16981 ttcggcaata tctgaaaatg aaagtttagc aagagttata gtagcatcct ttgcagctaa
    17041 gttagaccca acagtagatg agttagtaga tataaaaact gctgtctctg aggctgttac
    17101 gaatgctata atacatggat atgaagaaga cagttctaag tttgttttct taagatgtga
    17161 gatagaagga aatactataa agattatagt tgaagatgaa gggtatggaa tagaaaatgt
    17221 ggagaaggca atggaaccac tttatacatc aaaaccagaa cttgatagat caggaatggg
    17281 attcacagta atgaaaagct ttatggacga tgtagaggta agttctgtaa aagataatgg
    17341 gactagaatt gaaatgacta aaaaaataaa tgtgcctaaa taaagtccaa aagtgaggtt
    17401 ttattatgga agtaactgtt gccagagaag aaaaaaaacc tcttcttagt catgaagaga
    17461 ctctagaact tattgaaaaa gttcaaaatg gagatgaaga ggcaaaagaa atattaatat
    17521 caagcaattt aggtctagtt aggagcgttg tatctagatt tttaaatata ggatatgata
    17581 gagaagattt atttcagtta ggttctatag gcttaataaa atcaatatat aaatttgacc
    17641 caaaatttaa tgtaaaattc tctacttatg cagtaccaat gattttaggt gaaataaaga
    17701 gatacttaag agatgatggt atgataaaag tatcaaggtc gttaaagcaa atagctataa
    17761 aggcaaagat ggaaagtgaa gctttaacta aaaaattagg tagagagccg agtatagaag
    17821 aattagctaa agcaatagat gtagaaaaag aagatttagt aatggctatg gaagctaatt
    17881 tcaatgttga gtatttgcaa ggagttattc atgaagagga aggttctcca atatgcctta
    17941 ttgataaaat aagcatgaaa ggagaaagtg aagaagaaaa agtagtggat aacatattgt
    18001 taaaagacat acttggaaga ctggataaga gagagcgtca aataatagtt ctcagatact
    18061 ttgaagacat gactcaaagt gaaataggag agatgctcaa tatatcacaa gttcaagtat
    18121 ctaggataga gaagaaagta ttgtctaagt taaaagaata tatatcgtga taatacttat
    18181 gatatgtaga ataataaaat ataataaata tattactaaa ataaaatgta ataacttttt
    18241 atgttaagtt tttaaattat taaaagatat taaaataata aaaagcatta aaaaagtaaa
    18301 aatata
//
LOCUS       DAYASV010000075        18103 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYASV010000075 DAYASV010000000
VERSION     DAYASV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.075"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(4..690)
                     /locus_tag="ACGI68_002417"
     CDS             complement(4..690)
                     /locus_tag="ACGI68_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFH0138850.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(739..1272)
                     /locus_tag="ACGI68_002418"
     CDS             complement(739..1272)
                     /locus_tag="ACGI68_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFH0138851.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(1316..1948)
                     /locus_tag="ACGI68_002419"
     CDS             complement(1316..1948)
                     /locus_tag="ACGI68_002419"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HFH0138852.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(2038..3117)
                     /locus_tag="ACGI68_002420"
     CDS             complement(2038..3117)
                     /locus_tag="ACGI68_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFH0138853.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSDVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(3250..4596)
                     /gene="pepV"
                     /locus_tag="ACGI68_002421"
     CDS             complement(3250..4596)
                     /gene="pepV"
                     /locus_tag="ACGI68_002421"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFH0138854.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELNKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(4665..5945)
                     /locus_tag="ACGI68_002422"
     CDS             complement(4665..5945)
                     /locus_tag="ACGI68_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0138855.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(6072..6332)
                     /gene="hfq"
                     /locus_tag="ACGI68_002423"
     CDS             complement(6072..6332)
                     /gene="hfq"
                     /locus_tag="ACGI68_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFH0138856.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(6446..7381)
                     /gene="miaA"
                     /locus_tag="ACGI68_002424"
     CDS             complement(6446..7381)
                     /gene="miaA"
                     /locus_tag="ACGI68_002424"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFH0138857.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINKKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(7419..9386)
                     /gene="mutL"
                     /locus_tag="ACGI68_002425"
     CDS             complement(7419..9386)
                     /gene="mutL"
                     /locus_tag="ACGI68_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFH0138858.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(9397..12240)
                     /gene="mutS"
                     /locus_tag="ACGI68_002426"
     CDS             complement(9397..12240)
                     /gene="mutS"
                     /locus_tag="ACGI68_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFH0138859.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIENLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALIKQNDDKEKINQVDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(12400..13134)
                     /locus_tag="ACGI68_002427"
     CDS             complement(12400..13134)
                     /locus_tag="ACGI68_002427"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138860.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(13103..14116)
                     /locus_tag="ACGI68_002428"
     CDS             complement(13103..14116)
                     /locus_tag="ACGI68_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0138861.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(14138..14908)
                     /locus_tag="ACGI68_002429"
     CDS             complement(14138..14908)
                     /locus_tag="ACGI68_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138862.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(14883..15173)
                     /locus_tag="ACGI68_002430"
     CDS             complement(14883..15173)
                     /locus_tag="ACGI68_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HFH0138863.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(15304..15403)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      16023..16112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16165..16341
                     /locus_tag="ACGI68_002431"
     CDS             16165..16341
                     /locus_tag="ACGI68_002431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138864.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(16456..16812)
                     /locus_tag="ACGI68_002432"
     CDS             complement(16456..16812)
                     /locus_tag="ACGI68_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0138865.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            17189..17569
                     /locus_tag="ACGI68_002433"
     CDS             17189..17569
                     /locus_tag="ACGI68_002433"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFH0138866.1"
                     /translation="MTNADGLSESQMQKIARELNNSETAFIFNNLAKNHQENYDVHIR
                     FFTPTNEVPICGHATIAAHYVRAIENQFNNKTVFQKTGAGILPVDIIKKDNDYEIIMT
                     QGKIEVSPPLQQTYQKRLLNAFGI"
     gene            17629..18027
                     /locus_tag="ACGI68_002434"
     CDS             17629..18027
                     /locus_tag="ACGI68_002434"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HFH0138867.1"
                     /translation="MIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHNKETLQ
                     VHGRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPTPNTEFIFEAVQGEAIKRKGKM
                     LVKVIILDNKPVEVKIIGKAVIAFSTNIEI"
BASE COUNT         5803 a         3064 c         1948 g         7288 t
ORIGIN      
        1 tttttaaatt attccaaata cgccaaataa tattggtata aatatagctg gtaacaaatt
       61 ggctactttt atattcgtta atttaagtac atttagacca agtcctataa ttaataaact
      121 tccagctgct gtcatttctg tgactacagg atcacttaaa aatccattta aaaatgatgc
      181 tcccacacat ataatacctt catacaaaaa tacagatatt gcagaaaaca ttacacctat
      241 acctaaagaa gcagtaaata taattgaagc tattccatct ataacagatt ttacaaataa
      301 agtatcatga cttcctgtaa gaccactttc taatgaacca actactgcca tggctcctat
      361 acagaataat aaactagatg atataaaacc ctctgctata gaatcttttt tatttccttt
      421 agaaaattta ctttgtaaaa aagcacccaa cttatttacc catttgtcaa tatctattaa
      481 ttctcctatc agagcaccta cagctacgga tattattgtc actaatgtgt tttttccttc
      541 tagtgctcct gatataccaa tgtacaatac acataaggca attccattca ttatagtatc
      601 gcttactctt tgaggtaatc ctccttttat tataagacct actatacatc cacctgcaat
      661 tgctagacta tttgctatta ctccaaacat tttttctccc ctcgattagc taattgaaat
      721 ttatcctatt attaaaacct aaacatctat actttgtatt ggatatttta aatattccaa
      781 aaattcttca aaaccttttc tagatatatt tacagtttta gtatttatgt taggatgaaa
      841 atttaagtaa tcttctttaa ttacatcttt atctatgtaa agctcaactt ttctttctgt
      901 atcatttatt aatgaaaaag gatttacact tccaggaaga agtttaagta cattgtatag
      961 tctttcaggt gaagcaaacg ataatctaga acttcctatc ttatctttaa gtgagtttaa
     1021 atcaacttgt ttttctcctt taactacaat aagataatac ttatttcctt tggcatttct
     1081 taaaaataaa tttttacatt gacacccctt tgttattcca tctaaaatga ctaattgctc
     1141 tgctgtatat acagcttcat gctctactac atcatattca acatttaatt tatccaaaat
     1201 atcatatatt ttagtttcat tcatgctaga ttcattttca tcttgttctt ttttattatt
     1261 taaatcactc atacttttct ccttaaacac ttcatcataa tatcattgtg aggtattatt
     1321 ctactatagc tattgctctt actggagctc catcagcatc attatactta aatggtgctg
     1381 ctataaatag aaatttttct ggaacatttt ctaaatttgt aagattttct atgattattt
     1441 ttcctttttc aaataaaata tgatgcactt caaatactga tgtgtcatat ctatccactg
     1501 atagcatatc tattcctatg ccttttatat tagtattagc tatatagttt gcagcttctt
     1561 ttgttaatgt aggataacct acatagtact gctttttatc ccaaaatttt gaccaacctg
     1621 atttaaatat tataaaatca ctatttttta tttcatcttc atattttttt agatattcta
     1681 attctatatt ctcatttccc ttttcaagtt ccaatacata agctttccca acaaaatttt
     1741 ctatatccaa cttatctagg gtctcacctt tagtatacat atgtcttggt gaatccatat
     1801 gagttccatt gtgagaaaat acactaatta atgtttcttg ataaccattt tcttcaataa
     1861 tagctacttt cttgatatct ggtctattag gctctgcata tacaggcatg tcattatgtg
     1921 tcacatgagt taaatcaaaa accttcatct ttatataccc cctatgtttt tattttctat
     1981 tatataccta aattcatttt atttctatca gattttattc cattatatac catactacta
     2041 tatatttttt ataaacctca agggatttac cgaaaaatct tggaaagaat tatcagtaaa
     2101 tataacatct tcacccaaaa aacgtttata ccttccttct tctttcttta aagtaattct
     2161 ctgtttttgt gaatgattat taaaaaccat tatcttaaga ggaggttttt ttctattagt
     2221 ctgcactctt tttaacatgt attttatgtg catatcagct tcaggaaatg aatatccaca
     2281 aaatattaaa aggtcagtat ctcttaaagt tttttctgtt tcattccaaa catcacttaa
     2341 aaatatgttt gacatattct taaaatatgt tggtggcact ataataggtt ctgttaactc
     2401 accacactct aaacattttg tctctgaact attttcaata agcttcatga caccaccttc
     2461 atgtggtgta agagttacac tattacaaat ggggcaataa agccaattca atgacccatg
     2521 tatcttatat aatttgatta atggttcttt tggttttctc caatttaact cctctttaaa
     2581 atttgtaaac tccacaccat aatcaagcat aattggcaaa tcattcttat ctcttaatcc
     2641 agtaactgta ttgtctatat gtatatcata atttgcacta ataaaagtag tatcaagaag
     2701 caaatcattt tccaaaagat tttcaactaa aagtttatga tatttattat ctgttctagg
     2761 tgtattatga attgcttcag ccattagcag tattaaatat tgtcttaaaa aacgtatact
     2821 atcacttctg ttatttagat tttctagtcc aaaatttcta aatgcttctt ttctttgttc
     2881 tgctaagtct aaaagaccta aaacttcttc aaaggtagga aaatttgcat tatctatatt
     2941 atcttttact atatcaatat tgaacatctg tttaaagaaa atatacaatt ctctattcat
     3001 tttggtatta taatttgtgg gatttagttt tttaaaatat tcgctaaaaa gttcattttg
     3061 aataggtaga ttctctgctg ctgaagctcc tgctcctaaa aatattgcta ttttcatata
     3121 gttcctccta atataaatat tactcctatt tttatatcca ttaaatatcc atatcattta
     3181 ttttatgata caacaataaa gctgtctttt actagaaaaa atcctattac aagacagcct
     3241 tatattatat taaatcttta ataattcgta aatagaaaga gcatatattt gagtagcttt
     3301 tataatatcc tctactatta agtattcatt agcttggtgt tctgtttctg gttttcctgg
     3361 aaaaatagct ccaaatgcaa cacaattatc taaagctctt gcgtatgttg ctcctccaga
     3421 tgaaagtggt gttccatcaa gaccagtttc ttcttcatat acttttctaa gtgtcttaac
     3481 aagaagagta tcttctggaa catagattga atccaagaaa tcatattctt cataatttaa
     3541 attgtattta tctgtcattt tctttaactc ttttacaaaa tcatcctttt tgtatgttac
     3601 tggaatcctt acatctattc ctgcaaattc tttttcgtta tcaatagtta ctctacctat
     3661 atttactgtt agtttacctg atacatcatc cttgcagtta ggaattatat tgtttccatt
     3721 agcatcttca ccaataactt ctgctaaaaa cttaattata ttagaatcta tacctatatt
     3781 attaagagct atacaaagtc tagccactgc atttatacca gtatcacttg ctgctgaatg
     3841 gacactcttt ccaattatac agattttatt accttcaaca gtatactcaa aatttaattt
     3901 attaagttct ttttttaatt tatcagagta ttttccaata tagattgctt tacctggaac
     3961 tgcatttaaa gcctttccaa ctgacaactc tatatcactt ttgctatcac aagtcaaatg
     4021 aacttgtaaa agacctttct ctgcatttgt tattgggaat ctagaatctg gagtaaatcc
     4081 atagtttggt atttcttcat tattttcttt atatttattt atacatctcc aaagattttc
     4141 ttcatcagcc ccaaaaatga atcttatcct cttattaaag tctacattta aatccataag
     4201 agctttcact gcataaattg ctgatattgt aggtccttta tcatcttgtg ttcctcttcc
     4261 atacaattta ccatcttcta aaacagcttc aaatggaggg taattccagc tttctaaatc
     4321 accttctgga accacatcaa cgtgaccaag tatacctatc attttttcac cttgtcctat
     4381 ttctgcatat ccataatatc catctttgta tactgtctta aatcctaaag atttacataa
     4441 atctaatata cctcttaaag ctttatctac attttcacca aatgggcaat tttcagttgc
     4501 ttcagatata acactaggta tttttacact ttcttgtatt gaagaaatca tttcatcttg
     4561 tagagaattt actttttctt taatttgctc tttcatatta tcacccttca attttcatca
     4621 taatatacta ataaatattt tgacctttta aaaaaaaaat atgcttattt tttcagtttt
     4681 tttataaatt caatcgcttt taaagtcccc atttttgaat ggtcaaatgt aagtccacct
     4741 tggaaataaa cattataagg aggtcttatt ggagcatctg cacttaattc tatagaagaa
     4801 ccttgtataa atgctcctgc tgccattatg acttcacttt cgtatccagg catgtcccaa
     4861 ggaacaggtt ttacataaga atcgactggt gcagcttctt gtataccttc acaaaaactt
     4921 attacttcat cagcattatt aagtctaata cattgaataa tatcacttct taagtcatca
     4981 tattttggta atacatcata tcctaatttt tcaaaagctc ttgagcaaaa gatagctccc
     5041 attacagcct gagatactat ataaggcgcc aagaaaaaac cctgaagtac atttctagtt
     5101 gttccaaaag taagaccaca ttcttttcct attccaggag aagtcattct ataagatata
     5161 agctcaatca aatcttttct accagctata tatccacctg tcaatgcaag cccaccacca
     5221 ggatttttta ttaaagagcc agccataaca tctgctccaa catcagtagg ctctttggta
     5281 tctaaaaact caccataaca attatcaacc attactatag cttctggttt aacagacttt
     5341 attacttcta tagcttcttt tatatcacta attgaaagtg attttctcca agaatatcct
     5401 tttgaccttt gtatcataac gagtttagtt ctatcattta ttttgttttt aattccttct
     5461 aaatctaagt ttccatcctc taaaaaatct acatcatcat atgtaacacc atactctttc
     5521 aatgaacctt tttcttctct tatgcctata actccctcta aagtatcata tggtctacca
     5581 gtaacagata aaatttcatc tcctggtcta actatacctt gtatacataa agtaagtgca
     5641 tgtgtaccat ttacaattat tggtcttaca agagcatctt ctgtattaaa tactttagaa
     5701 taaatttcct ctattttttc acgaccaata tcattatatc catatcctgt agtccagtta
     5761 aagtgcatat cacttagttt agattcttgc atagctttta aaactttata ttgattatat
     5821 tctcttattt cttttatttc ttcaaactta tcttttattt cttccattat ttcttgcgat
     5881 agtttaaaag tatcatcatc tattccatag tagtctttta ataattcttt tgtctcatta
     5941 agcattttat cacctgtctc tttattattt tatttaaaca tgtaaaggag actattttac
     6001 taaaacaaat aaattatagt ttataagtta gtataagtct cctctcaatc atcttaaatt
     6061 aaattaatta tctatctgtt gttattatta ttgttgtttt ggttttgatt tgttagatta
     6121 atatactttc caggttgtat agttgataca gcatgtttat aaatcatgtt ttgttgtcta
     6181 ttatcccctt ctattaatat tatataacta tcaaacccct ttacaagccc tttaacttgc
     6241 actccattaa ctaaatatat agtaacaggt atcctttctt tccttgcatt atttaaaaac
     6301 aaatcttgta aatttaaaac tgtatttttc attagatttg taccctccca agattaaatt
     6361 gaacttctaa aatattccta ccttatattc tatataagtt atgacaaatt ttcaatatta
     6421 agttgtatac ttaaaaattt atatattatt ttatagaatc tttaatatat aagattattt
     6481 cattttttaa ttcgtctata ttttcatatt ggtctaaatc aaaccatttt gctgttttat
     6541 atcttttaaa ccatgttatc tgacgttttg cataccttct agaatctctt ttaattattt
     6601 caatagcttc ttcgtatgta tattctccat ccagatactt taatatctct ttataaccta
     6661 taccttgcat tgatatcata tcttttttaa atcccatact taaaagtttt tttacctctt
     6721 caaccaatcc atttttaagc ataatatcaa ctctcatatt tattctttga tacaaatgtt
     6781 ctctatctct atttagaact attattattg gttgatattc ttcattaaat tttaaatcac
     6841 ttgaaaaatc attcattttc ttaccactta gacaaacttc aagagctctt atgacccttt
     6901 tagtattgtt tttatggatt ctattagcag attcttcatc aagttctttt aacttattat
     6961 gcatgtaatc tattcccttt tcagcaagtt gtttttgaag ttcttctctt aattcattat
     7021 tggcatcaga ttgtgcaaaa tccatattat atattaaaga gtttaaataa agtcctgtgc
     7081 ctccagttac taaaacacat ttttcctttt tatttatttc atgtatatat ttttttgctc
     7141 tttgttgaaa ctctgaaact gaaaatggat agtcaggctt aacttcatct ataaggtaat
     7201 gcttcactcc ttgcatctct tcctctgtaa cttttgcact tccaatatcc atatattcat
     7261 atatttgcat agaatcagct gaaattatct ctgcattcat atcttttgct aattttattg
     7321 acaaatctgt ttttccaaca gcagttggac ctgtaagtat tataagtggt atttttttca
     7381 taacattcct ttccatatat tttaaatatc caaatatttt acataattct tttaaacatt
     7441 ttttctattt ctgtcttaga tatttcgacc attataggtc ttccatgagg acatgtaaat
     7501 ggattctcac acttttctag ctgttccaat aaacttttta tttcaatatc gtatatttta
     7561 tcatttgctt ttatagcaga cctacatgcc atagatgcaa atctctctcc ttttaaatca
     7621 taattacttg taatttcttc tatattatca attatttgaa gtataaattt ctcggtttca
     7681 ggtactccaa atatagtagg aacacatctt accattatat gattattgcc aaaaatttct
     7741 agctcaaaac caaatttcat aaataactcc aagttatttt caatttgaag catatctaca
     7801 tttgatactt caataaccac tgggtctaaa agtatttgca tatttatatc ttgtctatag
     7861 aacttctcca tatatctttc atatagtatt ctttcatgtg cagcatgttg gtccaacaaa
     7921 tacatagaat cgtctttgct caaaattata taagtattaa atacaactcc aattacagaa
     7981 tatccgtata aagaaaactt gcgttttgaa ctacacaagt attcttcttc actgtcctta
     8041 atactatctc ctaagtaata atttccttca tttttaattc catcaacttg aaactcttct
     8101 tgtatattat catttgcact atcttcactt agataattaa tgttttttac atcatcttct
     8161 acacttgcat ttaggacttc acttactgat tgaaattcat ttattggttt ttcacttgat
     8221 aaagtaacaa cttcaatgac ttcatcctta cctttagtaa tattactatt tttaggagta
     8281 gattctaata aatcattatt tcttaattta taatctgtac ttttttctct actgttaata
     8341 gcgattctag gttgtgtttt tttatcacta taagtagcat attttccaat aagatttgag
     8401 tgtattaatt taacctttaa aaaatctcta agttctatat acacttcttg ttctttttca
     8461 aatttaattt caagtttatt ggggtgtata tttacatcta tacatgatgg gtctacttct
     8521 atatttaaga agcacactgc atgtttacct attggaatta tagacttata actttctgtt
     8581 attgcatcta tgattatttt actttttaca aatcttttat ttatatagat atgttgtaag
     8641 tttttatttg aacggtatat attgttatta cctatatatc cattcatttt aaagtgattg
     8701 cacttaaact ctacatctat tatattttcc gttatttctt ttccatatat acttctaata
     8761 gtgtttacaa gctttccatc ccctggagta tttagcatct gtttattgtt atttgtatat
     8821 ttaaattgaa tgtttggatt acctattgca agtttattta tcaaatcact tatatttata
     8881 gtttctgcat gagtagattt taaaaatttc tgtcttgctg gtgtattaaa gaaaatatct
     8941 tttataatga tggtagttcc attagtagaa cctattggtt ctttcgatat tatttttcca
     9001 ccttcaacat atattttagt tcctattatt tcatctttag ttttagttgt catttctaat
     9061 tttgatacag ctgaaataga tgccaatgct tctcctctaa aaccaagaga gtataaatca
     9121 tacaaatcat caattttttt aattttacta gtagcatgtc tcaaaaatga tttttcaact
     9181 tcacttgaag gtattcctat tccattatct gtaatcttaa ttagactttt tcctccatca
     9241 attatatcta tggaaatttt atttgcacct gcatcaatgg agttttcaat aagttctttt
     9301 actacagaag aaggtctttc tacaacttca cctgctgcta tcttatttat agtcaaatca
     9361 tctaggatgt taattatatt tttcattttt acacctctaa gattttattt cttttgcttt
     9421 tctttgaaga ttataaagag agtttatagc atctaatgga gtcatattta aaatatccag
     9481 atttaaaatt tcttctgata gtatatctct atttacacta tcaaaagaga tttgagttaa
     9541 ggctacttct tttacagaat ccacttgatt aattttctct ttatcatcat tttgctttat
     9601 taaagcaact tcatcattta atgctttaaa tttagaattt aatttatcat aatccttttt
     9661 tatatgtttg tagtctttta ctataagttc atgttctatt tttagagatt catatttatt
     9721 cttaaaatta ctttgattta ccttaactaa ttcttcgtcc aaattactat taattatatt
     9781 attgttttct ttatctccat ttattgcaac actgttgtaa acatgatttt tttccaagtc
     9841 tttaagtata tattttgctc tttctatgac ttcgtctggt aatttagcta atttagcaac
     9901 atatattcca taactcttgt ctgctccctg aggaattatt tttcttaaaa atattattcc
     9961 ttccccatct tctttgactg ctattgaata atttttaacc tctttaaact cttcttctaa
    10021 atcagtaagt tcatgataat gtgttgcaaa taatgtctta catctaatat ttttttgaat
    10081 atattccact attgaccatg ctaaacttat accatcatag gtactagttc cacgacctat
    10141 ttcatctaat ataactaaac ttctctctgt tgcatttttt aatataaggg atacttcgtt
    10201 catctctacc ataaaagtag attgaccttg agataaatcg tcactagctc caactcttgt
    10261 aaatattcta tctaatattg gaatatcagc agactctgct ggtacaaatg agcctatatg
    10321 tgccataaga gctattatag cagtttgtct catatatgtt gatttacctg acatattagg
    10381 accagttatt atgttaataa tgttttctcc acgatttaaa taagtatcat ttggaacgaa
    10441 attttcctct cctactatat tttcgacaac aggatgtcta ccatttctta tatcaagttt
    10501 attattttca tttattgctg gttttacata gttatttata tgtgcaactg ttgcaaggga
    10561 cacaaaaaca tctatattag ctatagtctt agccactttt tgtattctat ctatgttttt
    10621 atatattgta tctcttatct ctacaaatat ctcgtattct aaagatttaa tcttttcctc
    10681 tgcatgtagt atcttttctt ctatttcctt taattctgga gttatatatc tttctgcatt
    10741 actaagtgtt tgctttctta tatatgtctc atctagcttt gcctgtttaa agttagcttt
    10801 tgttatttct atataatatc caaaaacttt attaaagcct atttttaatg atttaactcc
    10861 tgttttttct ctttccctgt tttctatttc ctttacaaga aaggctccat tcttagaaat
    10921 ctctcttagt tccttaagtt catcactaaa atctgattta attatgtttc catcttttat
    10981 ggttatcgta ggttcttcta gaatagcttc atctattaaa ttgtatatat catctaattt
    11041 atccatttct gaaacatact cttttaggat ttttgcattt gataaattta tagtatcttt
    11101 taaattaggt aacttttcta tcgaattttt taagtgaatt aactcttttg gtgttactct
    11161 ctcaaatgct atctttccac atattctttc tatatcgtat atatttttaa gtatatcatt
    11221 taggtcttct cttaatataa agtcatcttt tatttcttct attacatcta atctattttc
    11281 aattttagat ttatttatca aaggttcttc tacatatttt cttaaaagtc tacctcccat
    11341 tgctgtagaa gttttatcaa gtacgtgtaa taatgaacct tttttcttat tccctcttat
    11401 agtttgagta agttctaaat tagttctagt aaacatatct agaaccatat attctgatga
    11461 attgtatata tttatattat taatgttaga tgttatttgt ttttgagtat tgtatatata
    11521 attcagaaga attgataagc ttgactttat tagaccttta tcatcaaact ttagtttttg
    11581 tagataaaca tctggaaaat attcttttaa tatatttata tccaaatagt tatcactaaa
    11641 actttcattt atgtatatat tacttacagt agctatatct ctaagatttt ctataaagtc
    11701 taaatcattt ataattattt cagttggatg gatttttgca atttcttcaa ttaccttatc
    11761 ttcatttaaa catgttgcat tagtttctcc tgtacttata tcaacataag ttagacctat
    11821 attagtacca tccttataca aagataataa ataattattc tttttatttt caagtaaatt
    11881 tccatctaaa actgtaccag gagtgatgac tcttataacc tctcttctaa caattccttt
    11941 tgctgtagat gggtcttcca tttgttcacc tattgctact ttatatccat tttcaactaa
    12001 ttttgatata tatgaattgg ctgagtggaa aggaacacca cacataggag cacgttcttc
    12061 taaaccacaa gcttttcctg tcaatgctat ttcaagagct tttgaagcta ctaatgcatc
    12121 ttcaaaaaac atttcataaa aatctccaag tctaaaaaac agtatacagt ctttatatct
    12181 attttttact tcaagatact gtttcatcat aggagttaat ttattcatat ctatttgcat
    12241 tatcaaacct ccttcttttc taattaaata ttatatcata gtagatgatt attattaaca
    12301 caatatataa aattttgaca tataactaaa aacaatccaa aatgtatact cacattttgg
    12361 attgctaagt ttaatgctta ttaaataaaa atattttttt tacaataaag atattatttc
    12421 ttttttaagt ttaatgatat tttcaaggtt atcttcattt aactttctac tatctataac
    12481 aaattctttc tttactattg atggtttttt tgaaataaca taaattctgt ttgatagcat
    12541 tatagcttcg tcaatatcat gagttataag aagtattgtt gaatttactt ttttcttaac
    12601 atctaaaagc catttttgca tggatgtttt agttaatgaa tcaagtgccc caaaaggctc
    12661 atctaaaagc attatgtcat tagaatttat aaaagttctc aagaagttag ctctctgtct
    12721 cattcctcca gaaagttctg aaggatattt atcttcatat ccgcttaatc caaacacctc
    12781 aaaataaggt tttaccattg acctagcttt actctttttt tcttttttta aaattagtgg
    12841 tagtgacaca ttatctatta tagttttata tggcaagagt aaatcttttt gatacatata
    12901 actatactta ccatttatat tcactttgcc actatcataa tctgtaagtt tagttataat
    12961 attaaaaata gtactttttc cacatccact tggccctaat atagaaacaa actcaccttc
    13021 ataaacatca atacttatat cttttaaaac ttcaatatct ttataagttt ttgatacatt
    13081 ttctatagat acttttactt ttttattgtg gtaaaaattc atttgtgtaa gcatcacttg
    13141 ccttcatatc cttatctatt aatttatatt cttttaagaa tgatgtatag ttgttccata
    13201 cactatcttt catttctccc catcgtggtg catcatcttt gtacttgctt gctaaatatt
    13261 cttgactctt taaagctagt ttttcatcta cttcaggtgc atgttttact aaaatttttg
    13321 ctgattcttc tggatttttt attgcatatt cataaccttt tgttgtagct tttaagaatt
    13381 ttttagcaag ctctggatta tctttaagca cagtttcatt agaaattata agtggtgtat
    13441 aataatccaa tctctcatct aaatctttta ctggtataaa gtttaagtca tatccaatta
    13501 aatcagcttt aactgcatcc caaccttcaa atatccatgc aaaatctaca gttttcatag
    13561 ctgcaaagaa atcatcttga ccagtattaa ctatctttaa cttattaaaa tcagctccat
    13621 ttttcttcat aactgcttta aaaacagctt cttctgatgg agagccccaa ccaccataag
    13681 tttttccttc aaaatcttta gctgttgtta tattcttttc ttttggtgat gcaaacccag
    13741 aagtattatg ttgtataaca gttgctacag cttttattgg aagtggatct tcactagtct
    13801 ttgcataagt aacttgttct tgatagctta taccaaagtc agcttttcct gttgctacta
    13861 aagtttctgc tccactttca ggtggttgta ctatttcaac atccaaacct tcttctttat
    13921 agtaaccctt atcaagtgct acaaataatc cagtatgatt tgtatttggt gtccaatcaa
    13981 gcaccatagt tactttttta gaagaagtat ctttatcatt gtttttgtct ttattgttat
    14041 ttgaagaaca tccagttatt gttgccaatg taaaaacact tactaaacct aatattccta
    14101 actttttaaa attcataatt gcctccctca aataccttta atctattaat ctatgtctta
    14161 taacaacttt ttttacagcc tgtacaattc ctacaaacat taaactaaat ataactacta
    14221 atatagtaga tgcaaatact ttatcaagtg catatgcact ttttgaccta agcatataaa
    14281 caccaagtcc tttcgttcca cctagccatt ctgcaacagt tgctgccatt acagcatatg
    14341 ttgcagatat tttaagtcca gaaaaaaatt tatccattgc cataggaaat ttaaggtgaa
    14401 tgaatgtatt aactttattt gagtccatag tcttaaataa atttaaataa tctttatcaa
    14461 tattctcaat gccatctaca aaacttatta atataggaaa aaaacaagta agcattacca
    14521 tcaatacttt tggcattgtt ccaaatccaa accatatcat aaagagtggt gctataatta
    14581 ttgtaggtat catctgtgta accaacataa taggataaag acattttttt attattgata
    14641 caaaatccat caatattcca attaccaatg atattataat tgcgactata aaaccaagta
    14701 ttgcttctcc tatagtaacc acagcatgca tggataaatt ttgataatct ttaaaaaaca
    14761 cattaattat atctactggt gtgggtagta tatactgagg aacttctaaa actcctacca
    14821 tagattgcca aagaataagt ataaacaaaa atgttgctat tggatatata ctatctttga
    14881 ctttatctat gatttctaag tttttcatct atagtcagac ctccagcttt ttcatctact
    14941 ctagctactg ttatgactct ttcacatcca agattaaaag ggattttatg tacctttttt
    15001 aataattcaa aaacttcatc aaaatcacct tcaacagtag tgctcattgc tcctatttca
    15061 catttaagac cacttttttc aatttcagca atacaagcat ctactacttt atacatctct
    15121 tcttcacctt tgtaaggtct tagtggcatt actgctatgt ctgcgattac catttaaatc
    15181 actcctttaa aaataattaa tacctactag gtatattttg ttttttatta aaaaatttta
    15241 ttactaaaaa gatattatat ttaaataaaa gaaaagtctt atccaaaata ggataagact
    15301 tatttttatc ttaactggtc tccctacact ggcattatcc aaacaggttc aaagggtcga
    15361 gataaaatct cctctcagca cattagctcc ccgaaatata ttatatcttt tctacttgat
    15421 tatactacac ttatcatatt ttaacaaatt ttttatttat attagatatt ttatccgttc
    15481 aaaaatatct aatataaatt gtacattatt acaccaaata aattatctta aaatggtatt
    15541 ttatcaacat cttccaaaac tagattaact gaattttcta gtttttattt tgctatttca
    15601 agcatatttg gatgtatatc aattccatac aaatattgtg ctaaatttct gttttcactc
    15661 atctaatttg caatcgttac agattacaga ttagaaacag tttttagact ctacttatct
    15721 ttatctacaa aaaatctttc aataaattca ttaataatat tcagtaatct aaaaataaac
    15781 ctctatctgg atttttaaga tacaaacctt ttaggaagta tcttttgtga taatataaaa
    15841 attactcgtc attatcatca aaatcctcag caaccaacat tttatataag aatcaaactc
    15901 tataatataa ttattttatc caatttattt ttgagaaata aaatattttt ttaattttta
    15961 aattttttaa tatgcacctt gaaaaaagaa acgttttcct atattatggt aatagtagaa
    16021 taaaactaaa taggcaaaac tagagaaatt tagtgacgca aagctatagg gactaagact
    16081 tatgaaatga gttatgtcag ccagttgcca aagagatatt ttctttttgt ttttatgaat
    16141 gttttttagg gggatatatg atatatgttt atggataaaa tggatagatg tacacatata
    16201 ctaactgcat atatatgcag ttcacgcgat tactgcaatt ttatagatac acaattaaat
    16261 gattttatac tagaatatgg agagaatgtg gtagaatctt gtttgcatca agtgatggta
    16321 ttggtaagca gatataatta aagaaatttt attttctaga aaaccctttt tagttttatc
    16381 tacgttttat aattcattaa agaactaatt aaaataagct tcattatata tttttcatga
    16441 agtaaacagt taagtttatc tcatatactg tttaccccaa ttacataatt ctaataatat
    16501 agattccaat gatttaccac gtttagtaga caaatattca acttttggag gaatagtcgg
    16561 ataagaaatc ctcttaataa gtccatctct ttcaagatac tttaattctt gtgacagtat
    16621 cttatgactt atatgaacaa cttctttttt taattgactg taacgtattg gctcttgttt
    16681 taaaatatgc cataaaataa ttgccctcca tttacttcct atcacagtca tggtaaaacc
    16741 aattgaacaa ttaaaatctt tttgtctttc ttcaatattt ggaaattctt caaaattatt
    16801 atctatatcc atctcaatcc tctctttcaa aatagtaagt atctaactaa aaagtgcatt
    16861 cttgtttagc gtactaattc gtaatataat cttgattaaa aaatagctat gcacactcgt
    16921 gactttagtc atgggaggtt cagtgtagca tagagcctta cataatacta cttttttaga
    16981 tgtatatgtt taaattttaa tatagacata tgttatttta ttaattttat ttaaacagat
    17041 aataataaat aataatttat ctcaagtttt ctatgtagat aaactaatct aatgtttaga
    17101 aaggattata taatatgaat aaaaaatttc gtctttatca agttgactca ttacaaaaga
    17161 aaaatttaca ggtaatccag ctggtgtaat tacaaatgct gatggcttat ctgaatcaca
    17221 aatgcaaaaa atagcaagag aattaaataa ttcagaaact gcttttattt tcaataactt
    17281 ggctaaaaat catcaagaaa attatgatgt acatatcaga ttttttacac caactaatga
    17341 ggttcctata tgtggtcatg ctactattgc tgcccattat gttagagcaa tagaaaacca
    17401 attcaataac aaaacagtat ttcaaaaaac tggtgctgga atattaccag ttgatattat
    17461 aaaaaaagat aatgattatg aaataattat gacacaagga aaaattgaag tatctccacc
    17521 attacaacaa acctatcaaa aaagattact taatgcattt ggtatttaaa ggaagatatt
    17581 cgtacagatt gtcctattgc catatcatct acaggacatt caaaagttat gattggaata
    17641 aaggaattat caacattaaa tagtttatct ccaaatttat atgaattatc gaaaataagt
    17701 caagaaataa aatgtaatgg atattatatt tttacattac ataataaaga aactctacaa
    17761 gttcatggaa gaatgtttgc tcctgcaatt ggtatttcag aagaccctgt tacaggaaat
    17821 gctaatggac ccttaggtgc gtaccttgta cattataaag taatacctac acccaacaca
    17881 gagtttatat ttgaagcagt tcaaggagaa gcaataaaac gcaaaggcaa gatgttagta
    17941 aaagtaatta ttttagataa taagccagtt gaagtaaaaa taattggaaa agctgtaatt
    18001 gcattctcaa caaacataga aatctaatta tagattcctt ctaagtaaat ttttgaactt
    18061 tttaatacta atttaataac aaactaattt atatacaatc aaa
//
LOCUS       DAYASV010000076        18014 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYASV010000076 DAYASV010000000
VERSION     DAYASV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.076"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            24..1304
                     /locus_tag="ACGI68_002435"
     CDS             24..1304
                     /locus_tag="ACGI68_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFH0138868.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNLAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDV
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1567..2958)
                     /locus_tag="ACGI68_002436"
     CDS             complement(1567..2958)
                     /locus_tag="ACGI68_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFH0138869.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2969..4189)
                     /locus_tag="ACGI68_002437"
     CDS             complement(2969..4189)
                     /locus_tag="ACGI68_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFH0138870.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4203..4991)
                     /locus_tag="ACGI68_002438"
     CDS             complement(4203..4991)
                     /locus_tag="ACGI68_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFH0138871.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5008..6282)
                     /gene="larA"
                     /locus_tag="ACGI68_002439"
     CDS             complement(5008..6282)
                     /gene="larA"
                     /locus_tag="ACGI68_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HFH0138872.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6400..7113)
                     /locus_tag="ACGI68_002440"
     CDS             complement(6400..7113)
                     /locus_tag="ACGI68_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFH0138873.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7238..8497
                     /locus_tag="ACGI68_002441"
     CDS             7238..8497
                     /locus_tag="ACGI68_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HFH0138874.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8777..9343
                     /locus_tag="ACGI68_002442"
     CDS             8777..9343
                     /locus_tag="ACGI68_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0138875.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9544..10749)
                     /locus_tag="ACGI68_002443"
     CDS             complement(9544..10749)
                     /locus_tag="ACGI68_002443"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFH0138876.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10852..10958)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11050..11418)
                     /locus_tag="ACGI68_002444"
     CDS             complement(11050..11418)
                     /locus_tag="ACGI68_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HFH0138877.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11470..12747)
                     /gene="obgE"
                     /locus_tag="ACGI68_002445"
     CDS             complement(11470..12747)
                     /gene="obgE"
                     /locus_tag="ACGI68_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFH0138878.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12858..13148)
                     /gene="rpmA"
                     /locus_tag="ACGI68_002446"
     CDS             complement(12858..13148)
                     /gene="rpmA"
                     /locus_tag="ACGI68_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFH0138879.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13162..13479)
                     /locus_tag="ACGI68_002447"
     CDS             complement(13162..13479)
                     /locus_tag="ACGI68_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFH0138880.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13494..13805)
                     /gene="rplU"
                     /locus_tag="ACGI68_002448"
     CDS             complement(13494..13805)
                     /gene="rplU"
                     /locus_tag="ACGI68_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFH0138881.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13906..15327)
                     /locus_tag="ACGI68_002449"
     CDS             complement(13906..15327)
                     /locus_tag="ACGI68_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFH0138882.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15345..16046)
                     /locus_tag="ACGI68_002450"
     CDS             complement(15345..16046)
                     /locus_tag="ACGI68_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HFH0138883.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGAESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16048..17892)
                     /locus_tag="ACGI68_002451"
     CDS             complement(16048..17892)
                     /locus_tag="ACGI68_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HFH0138884.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
BASE COUNT         5732 a         3293 c         1923 g         7066 t
ORIGIN      
        1 attaaagata aaaggagtat tttatgaaca tacttggatt aatagttgaa tataacccat
       61 ttcataatgg acacatatat catttatcta aatctataga aaaaacaaat gcaacacata
      121 ctgtagcaat aatgagtgga aattttctac aaagaggcga accagcttta tttgataaat
      181 acactagagc aaaaattgct gtcgaaaatg gtgtagactt agttatagaa ttaccaactt
      241 tatttgcttg tcaaagtgct gaaatttttt ctcatggtgc tataacaatt ttaaattcac
      301 taaactgcat aaattctata tgttttggta gtgaagaagg aaatacagaa attctttata
      361 atctagctga aattttagca aatgaaccaa atgaatttaa gttactttta aaaaaatatt
      421 tagatgatgg tctgttattt ccaacagcta gagctaatgc actttttgat tatatacatc
      481 aagacaaaaa aaataatttt atacaaaatt attcaaagga acaattatca aaaacattaa
      541 attcatcaaa caacatactg ggtatagaat acataaaaaa cctcatccat ttaaacagta
      601 gtataaaacc atttactatc tccagggtcc aatcagaata taattccgaa gaaattgaga
      661 gtgatatttg ttctgctact gctataagaa cttcacttaa agaaaataca gacctttttc
      721 atttaaaaaa tgttattcca gaaaaaactt ataacatcct aaatgaaaaa atagataatg
      781 gttttttacc tatgtttgat gacttcttct ttgatatttt aaaaacaatt atacttagag
      841 atatggacac tttaaaagag tattttgaag taaatgaagg catagaaaac agaatctata
      901 aagatatctt tacatctgaa tttctacatg aactacaatt agcaattaaa tccaagcgat
      961 acactttaac caaaatcaaa cgaactctaa acaatatatt gttaggtatt aaaaaaagcg
     1021 acgtgcaact tataaaaaac atagatacta taccttatat aagaatactt gcttttaatg
     1081 ataaaggtag agaaattcta aaagagataa aaaataaatc tgaaataaaa ataataaaca
     1141 agttttcaaa tatatcattt tcattagatg atacgatttt taaaacactc ataagttatg
     1201 atataaaagc tacaaatata tataattcaa tatattataa aaacaataaa gatttagtaa
     1261 aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtttttt aaaataacta
     1321 tatttataaa aatcatttta ttaactcttt attatataag atacaatagt cttactttat
     1381 ttatgtacac atgcttttag gatgacaaca tcaacttaaa tattatgtta ctttctaaat
     1441 ttaagtaaat attgccatcc taaaaatttt acaatttagc ttcagattta aaagtaaagt
     1501 ttaaaatatt atatttaaat ctaatttaat attttttgtt ttataaatat tattaaatta
     1561 atatttctaa tatattttcc ctggatttaa aatattttta gggtcaaaag ctaactttat
     1621 atttttaatt aacatcatat aagcatctga atttgattca tgtaagtact cttttttagc
     1681 atatcctatg ccatgttcac cagaaacttg accactaagt tctctagatt ttttatacat
     1741 atagtcaaaa gtttctttta atcttatttt ccatgtatta tcatccatac catcttttaa
     1801 aatatatatg tgtagatttc catctcctgc atgaccaaaa ctttttattc ttatttttaa
     1861 tttgtcttga agctcgtgag tgtatcttat aaactctgct atcttatctc ttggaacaac
     1921 gacatcacat tcatccattt gtgtcgtaga tgcctttata gcttctaaaa atgcacctct
     1981 ggctgaccat atagaatcgt ttctttcttg tgtatctgat ataaatacat ctaatgcacc
     2041 attttctaaa cataaattag ctactttttc atattctttt tctatatctt cagtgctatt
     2101 tccatcaaat gtaagcaaaa gataagcatc tgatgtatta tctggaaact ttttacctaa
     2161 aaattcttct gctgccaata tcacatctct ttccataaat tctatagccg ttggtattga
     2221 ttttgatttt attattttag gaacagtttc tattgccatt gataaatctg gaaatggtat
     2281 aagcaaactt atacttttct ttggtaaagg aagcaatttt aatatagctt tagtaactat
     2341 tcctaaagta ccttctgagc ccactaataa atcctttata ctatatccag aactattttt
     2401 aaccaccttt ccacctacat ttataatttc tccatttggt agtacaactt caagtcctct
     2461 tacataatcc cttgtaacgc catattttac tgctctcata ccacctgcat tagtgtttat
     2521 attacctgca attgtagcac ttttttcacc tgggtcaggt gggtaaaata aatctctatc
     2581 ttgaacatat tgacctattg tcatcaataa aacacctggt tctactgtca atgttaaatt
     2641 ttcataatca acttcaagta ttttattcat tttacataga ttaatcatta ttccaccatt
     2701 tatggcaaca gcagcaccta caagaccagt accttgacct cttggtgtga ctggtatatt
     2761 atttttatat gcgtatttca ttattttaga tacttgttca gtttcaagta catttactaa
     2821 aacttcaggg tacttctcaa tccctccaag ttcatcatgt gaaaaatctt catttatatc
     2881 acttccaact aatacatttt cttctccaca agtatctatt aaaaaatcta tatctttttt
     2941 atcgattaat ttatacattt ctatccccct atttagctgc tgatgccata tatttttgat
     3001 tattatcttt gttattttct attttttcaa tcaatttagg aataatatca taaatatcac
     3061 ctattaaagc taaatgtgct acatcaagta taggcgcttt atcatcttta tttattgcta
     3121 ctatatagtc agcaccctgc atccctgcaa caaattgaac tgctccagat acaccacaag
     3181 ttattatcaa tttaggtttt acagttctac cactaagacc tatttgtttt ttagcatcta
     3241 tccaacctgc ttctattaca ggtcttgttc ctgccacttg agcgtttaac ttatcagcta
     3301 atttatatat catctccata tcttcttgtt tttttattgc tctacttgcc acaactatca
     3361 cttcagcttc ttccagacca acttctttat ttttttcttt tacactcagt accttaattt
     3421 tagattttag ttcttttttt tctactttac ataaagttat ctttcctgta gtattctcta
     3481 ttttttctgg tgctgaaaat attttatatc ttacagtggc aaattgtggt ctattattag
     3541 gtgtgtttat atgagccatt atatttccac caaatgctgg tcttatttgg tctaaatcag
     3601 tatttgattg aatatcaagt attgtacaat ctgctgtaag acctgttcta aatctagcgg
     3661 caagtcttgg agctaatgac ctacctaatg tagttccacc aactaatact atcgttggtt
     3721 ttattttatc tataaagttt tctattgctt ttgaatatgg ttctatccta aaatctttaa
     3781 attcatcttc atcatataca aatacttcat ccaccccata agaaatcaaa ttactgcact
     3841 tatcttttat gtcttttcct acaaatacac aatacacagg ttgttttatt tttgatgcca
     3901 tctcacgagc tttacctata agctcgtacg ttacaggatg tatatttcca ttatgatgtt
     3961 caacatatac tgcaattccc ctccattcat ctttattaat ttgtacttta ctactctcta
     4021 taaattcaaa tgcgcctttt ggtccacttt tgacacacaa cttgcacatc ttacagccag
     4081 cacttatttc tactatacct gacttttctt ctatagcccc aaaaggacat atttctaaaa
     4141 ttctctttaa gtcagttatt ttatcttgat ttacaactat ctttgacatt tattactccc
     4201 ccttacacca actttaattc ctctaattta tttacaagtg caaaacttag ttcatctgca
     4261 tttcctctta caatattttt atctgtattt ggctttggag gaaatattct tacaacttga
     4321 gtaggagaac cattaagtcc atagttattt tcatctttat cttcaaagtc atctaagcta
     4381 tatacaggta tttctctatc tttagtatta agttttaatt taaatgaagg tagtcttggt
     4441 tgaaaaatgt ctttttctac agttattaaa catggatatt caatctcaca tacttgtaaa
     4501 tcatttggca tatctatttc aacaactata ctttcatctt taacttcaat taattttgta
     4561 acgttagtaa catgaggtat atctaaaaat tcagctactt caggtcccac ctgtgctgta
     4621 tcaccatcag tagtttgttt tccacaaatt attaggtcta cctttcccat cttcttaata
     4681 ccttgtgata tggtatagga agtagccaat acatctgctc ctccaaatct cctatcactt
     4741 attaatgctc cttcatcagc tcccatcata aaactttctt caattacctt ctttgcctga
     4801 ggaggaccca tgctaagtac ttttagattc gctttcttat cttcctttat tctaagagct
     4861 gtttctaaag catataggtc atagggattc atttttgaat ctactccatc tctttttagt
     4921 actcctgtag tttcatcaac ttctactgaa gtcgttccag gaacttgttt aatacatgtc
     4981 aaaatttcca taaagtacac tcctttttta tttaactata actgatactc catcaggtat
     5041 aactactact ttgctctcct taccaactat actattcgcc atttctaaag cttctttaaa
     5101 gttaaatgca tgtttcatat gcattttttt tatcatttca ggattacact tatctgttac
     5161 cattatcaca gtatatttag ataatattct cgctagtatc tgaaattccc attggtctgg
     5221 aatcgtatct actctatcga cacttctaat tttatctaaa agttgttttg gagaatctgc
     5281 atttgctaca ttttcataaa aagattgacc tccatgacca tcattacatg ctgaaatcat
     5341 tattattact ccaccgtctt tacaagtagc ttcagctgct gtcattcctt taacagattg
     5401 ataaatattt tggtctagtg gatatccacc atttgtagtt acaacaatat cagattttat
     5461 agaatttact tttgataact ctgttacaaa tctacatcct ttagtatgtg ctttttcgac
     5521 atcaccagca aaggaagcaa ttattttttt atctttatct attacaacat tcaatatgaa
     5581 tgctaatttt gcaactttag ctgcatatac catatcctca tgaataggat tattattcaa
     5641 ttttccagtt cttgaattat cactatctat aaagtcacta caatgattgt acattatagt
     5701 ctttgcagat gcgattcctg gaagtacact ttttctgcca cctgaaaaac ctgcaaagaa
     5761 atgaggttct ataaaacctt ctgctatcaa aagttctgcc tcatatgcta gtttgtttat
     5821 atataaatct ccacctgaag gcaatatccc tactttacac atagatttat catcagtaga
     5881 tatatgcata actatatctt cattatctac tatttcttct cccattttat aaattagctc
     5941 ttcccttgta cttggcctat gaaatccagt tgccactata atcttcacat caatttctgg
     6001 attaacattc ctaatccttc ttaatataat tggcatagtt attctacttg gtacaggtct
     6061 tgtatggtca cttgtaatta tgactacatt ctttttcccc ttaaccagtt cttccagaga
     6121 attagagcct ataggattat tcatagattc ttcaactatc tcttcttgag tcttatgttc
     6181 tatataatta tccgattttg actccaaaac accgagaaaa cgttcatcag gtatttctaa
     6241 aatcattcct tgttttgaat acggtacttt taactttacc atttttaatc tcccattata
     6301 ataaactata aaattggtat gtggtcatac cagttttaca tttatatgat agtaccatta
     6361 tctaccattt gcaagaataa tttattttat aacaaacttt taattttcat tttttctata
     6421 atttttactt ataaaatcca tatgttcctt tgctttctca catgcaagtt ctacatcatt
     6481 ttctacaact gcattgtata tagcttcatg ctgtttaagc aatatatttt cattaaaagg
     6541 ttctgcgatt attttttctc ttgcatcttc tataaatcca tcaaataatt ttgatgctgt
     6601 cataaaaaga ctttgaatta aataattttt acttaatgtt gctagtttac tgtgaaattt
     6661 ttggtctaat aacaccagta ctcgattatt atctttttta ttagtttcta ccctcatttc
     6721 atctattata tgtttcaatt ctataacttc agaagaagtt attctctttg aagcaagttt
     6781 tgcaatctca atttctagga ttattctaag ctctaatata tcttcaagtg taccattatt
     6841 aagtttaaaa atcatagata aaggttctat taaagttttt tctatatttg tacatataaa
     6901 atttccttca ccctgtctgc tttctatcac tcccatagtc tctaataccc taatagcttc
     6961 ccttatggaa gttctactca cattcatttg ttcagacagt tctctttctg atagtaattt
     7021 atcccccttt tttaactctc catttagtat tttatattgt atttgctcaa ttacttgttc
     7081 ataaactttt ttactagcta tactattaaa cattttttcc tccaatatga taatcgttgt
     7141 tgtaatatat atctattata tatcttaagc cattacattc atatattttt attactttta
     7201 atataatatc ttataactca aatttacagg tgatattatg aaaaaaaata aaattattgc
     7261 tttttttgct ccatctatca tagttggagt tctagtttta gggattatcc tatacccaaa
     7321 tgaagctata aaatctgcaa gagaaggctt ttctatatgg tctaatgttt tagttccatc
     7381 actactacca tttataattg gagcaaactt aattgttgat ttaaaaatag tggatattat
     7441 agggtttatc atcaatccca taactagatt tgtatttaat gtttcaggaa agagcgcttt
     7501 agcttttgct atatctacag tatctggtta tccagtagga gtaagtttag cttctgaatt
     7561 taggagtaat ggacaaattt ctaagtatga agcacaaaga cttgtgtctt tttgttctac
     7621 atcaggacct ttattcataa taggttcggt tggtacaggt atgttccaaa attcaaactt
     7681 aggttattta atgattctct gccattatct aggaactata ttggttggtc ttttctttag
     7741 aaattatggt aatgaaaatc ttccaaagac aaaattggat ttaaaaacaa atataaaaaa
     7801 tgtaatcaac attagaaatt caagtgggaa tggttttttt gttttatttg gaaatgctgt
     7861 atttaatgga gtaaatacat tacttacagt tggtggattt gtaattgttt tttcagtagt
     7921 atttaaaata ctatctcttt ttaatgtaat ctcattaata gcatcagtta tatatttgcc
     7981 tctttcattt ttaggtgtta gtaaagaact ctgtcaagca tttgtaagtg gtctatttga
     8041 aattactata ggatgcaata aagtatctag tataacaagt tctccagaaa ttttaagagc
     8101 atctttagct agttttttga taggatttag tggattatcc atcctagctc aatgttgtac
     8161 atttctatca aaaacggaca tagatttaaa attatacata ctaagtaaat ttgcgcatgg
     8221 agttttagct gctatcttta tatttatact ttatccaatt gcaaactctg ctgtgttggt
     8281 ttctagcttt gctgatacat acaacgttat atataataat ttaatctggt tttattatct
     8341 tagctattat gatacaattc ttcaaattgt agtaataatt tatctaatct cagccgtatt
     8401 tattgcaaaa aaacatatga aaactttatc tacaaatggg aaactaatgc gatataaaaa
     8461 tacttttttc actaagaaat tttcacgaaa aaaataaaac taaaaataaa actaataaaa
     8521 cttaactaaa tacttccatt tttttattat atttgtcata attttaataa cctttttaat
     8581 tagtttaaca gcatagttat ttcatcacaa tttaataatt ttattagaaa tatttttcaa
     8641 aaaattctcc ttaatcacat atatcacact tgtataagtt tactgggaaa tttttccatt
     8701 atataatatt tcatttatat tatataataa ataccgacct aaacttacca aatcaaacta
     8761 agaggagtgg ttgaaaatgg caacaagacc tatagaaata gacgaatata aaaagataat
     8821 ggaactatta catactggct ttacttatag tgaaaatggc gttgaaaaaa gatttagaaa
     8881 aaatcctaaa gttgcattgg cactaatgtt agaagctaat ttaggtctaa gaatatcaga
     8941 tattttaaga ttaaagatag gtaattttaa aggtaatatg ttggaaataa atgaaaaaaa
     9001 gacgggcaaa ttacagtata gaccagttaa taaaaatata attgagtcca taaatgaaca
     9061 tgcaagaaag tataatctta aatcaaatga ctacttagta aatataaaga caaaggctat
     9121 tcaaaaacag ttgaggataa tttgtaaata cttaaatctg tataatataa gtacacatag
     9181 ctttagaaag ctctatgcta ctactcaatt tgaaaagagt aacaataatt tagagttggt
     9241 taaagaactt ttaaatcaca gctctgtagc cactacacag cgatatataa gggtaactca
     9301 gcaagctatt aatgaagcta gtgaaaattt ctttattggt taattataac gtatacattt
     9361 ttctattatc ttattaattc accagatatt aaaaaagagt gtctcaaaat gaacttttta
     9421 gttcatgaga cactcttttt ttataaaatc aaatatattt ttattatttc aacactggaa
     9481 aaaaggctta tctctctatt ttgagacacc ctattaaatt ttcataattc ttgcataaac
     9541 atattaatct atcttcaata attttttagc tgattcactg taattatttc tttttacaat
     9601 ttccaaaact ctatcaatct catgttctga ctttgcactt acaaaaatcg ccaattcttc
     9661 atcatctata atatcataac tatctacata tttatctaaa cattctaaaa ttccatcttt
     9721 attattatac ccaccaaatc taactactgc actacactca tcttctataa gtccacttaa
     9781 atccaatttc ccttttgaaa aagactttat atttgaactt ctattatcta ttatatctat
     9841 aatatctgca taaacagcac ttcctgttgc acctcttcca gctcccttac ctacaaaaga
     9901 aagttcacct actgaatcac ctactaaaat aactacgttg aactcattat ctatctttga
     9961 aagcatatta ttattgtcca ctaatgctgg tctaacaaat ccacttactt ctcctgattc
    10021 atacttactt ctagcaacca attttatctt acaatttaat ttttttgcat attctatatc
    10081 tttttcatct attgaagtta ttccttctaa taataaatct ttccaatata ctatttttcc
    10141 ataagataaa gtagacaata tagataactt tctagcacta tcatatccta aaacatctgc
    10201 atctggatta acttctgcaa atcccaactc ttgtgcctgt tttaaagcga catcatacgg
    10261 taaattttca tcatacatct ttgataatat aaaattacaa gtcccattta aaatagcgtt
    10321 tatgctcttt atattgttgc cttccagtga ttctatcaat ggttttaaaa ctggaatacc
    10381 acctgccaca gatgcttcaa acttgatgct aacattattt aagttagcta attctataag
    10441 gtcactacct ttttcagcca gtaaatcttt attagctgtc actacatgta tttttttatt
    10501 taacgctctt gttacataat caaaagctgg atttaatcct ccaattactt caactagtat
    10561 atcaatatcc ttgtcaaata tatcttctat atcagtagtt atcttatcaa aatacttctt
    10621 atacttgtgt ttttctttat ttttaactaa aattccaaca acttctatat ctctttttga
    10681 agtattattt tctattaaat caattaatcc acttcctact gttccatacc ctaatattgc
    10741 tatcttcact gtaacatacc cccatcattt tgattatatt aaattattta tatatataaa
    10801 tataccaata aaaaaagctt gagtataaac tcaagccata tgacgggttt atactcatct
    10861 ttcagcataa atgctgcagg atttagcacc aatgtataat taaatatact ggttgccggg
    10921 cttcacaggg ccagtccctc tgccactctt gataagattg ctaatttaaa tttatgttta
    10981 atagtataat acaatttctt ccaattgtca ctattttttt aacttatttc cctaaaagga
    11041 tataaactat tatctaacag ctcttttagt tggtttaccc ttttttgtaa cattattatt
    11101 tttagtcttt gctttcgcct gttcatgtcc tggaaaaagt attttaggct cttcaatatt
    11161 cctcttgtaa atagtaaatt taaaaccaat agcctgcaca aattcagctc ttaaagcttc
    11221 acatactgca ttagcagttt ctttagtatc aagacctgca ttttctaata tagtaacttt
    11281 tactaattct ctagttctta acataccatc taattgctct aagaaactct ctgtaacacc
    11341 ttctttacca atttgtgtag taggctttaa agtatttgcc aaagatctta aatacgccct
    11401 ttgttttcct tttaacatat tctcacctct ataattctat acttaaatta tctataatct
    11461 atatttaaat taattgtaaa actcaaattc taattcataa attttaacca catctccatc
    11521 ttctattccc atttctctaa gcctatcaaa aacaccttga ctctccatag ctttttggaa
    11581 atattgaagg gattccatat catcaaaatt tacagaatac attattcttc taagtgcttt
    11641 acctgtaact acatacacac catcttcaat atctatactt aactcttctt cagtaccaat
    11701 atcaagttct ggtctataca tttcttcttc tgaaactagc tccacatctt ctacttcttt
    11761 tagcatttta gaaacatagg ctattacttc atctatgcct tgacgagttg ctgctgacat
    11821 tttaaatact gtatatcctc tgccttctaa ttcactctta aacttctcaa acttactctc
    11881 atcttctaat atatcaaatt tatttgcaac tacaacttgt ggtctctttg aaagcttttc
    11941 attgtacaat ttaagctcat catttatctt gtcaaaatct tcaatagggt ctcttccctc
    12001 taatccagat atatctacaa tatgtatcaa tacctttgtt ctctcaacat gtcttaagaa
    12061 ctcatgacct aatcctatgc cttcagaagc accctctata attccaggta tatctgcaag
    12121 tacaaaacta tctccaaact tagtttgaac aacacctaga tttggtgtaa gtgttgtaaa
    12181 atgatagttt gcaattttgg gtttagcttt agttacaact gatagaaatg ttgatttacc
    12241 tacatttgga aatcctaaaa gaccaacatc tgctatcatt ttaagttcaa gagttatcca
    12301 tctttcttct ccatctgtac cagattttgc aaaagctgga gcttgtctta ctgcatttgc
    12361 aaagtgttgg ttacctttac cacctatacc accttttgca actatagctg tatctccttc
    12421 tttttttaaa tctgctaata caagacctgt agcctcatct cttattactg tacctgctgg
    12481 tacttttaat actaaatttt ctccattttt accagctctt tttcctttgc ttccgtcgcc
    12541 accattttga gcttgatatt ttttctggta cttaaagtcc attagagttc taagccccaa
    12601 atcaacttca aatatgatgc ttgctccacg accaccatct ccaccgtctg gaccaccagc
    12661 tggaacgtat ttttctcttc taaatgctac cgaaccattt ccaccatttc cagccttaac
    12721 aaatattctt gccttatcta taaacaaatt cttcacctac ttaattaaaa acttttaatt
    12781 agcattacca aaaataccat tataaattca tcagtattat aagaaaaagg agtgcctcaa
    12841 cactcctttt atgtatatta ctcagctatt gctactggat atatactaac tttttttctc
    12901 ttcttgtctt ttctttcgaa tttaacagta ccatcaacta atgcaaataa agtatcatca
    12961 ctaccttttc caacattagt tcctggatgt attttagttc ctctttgtct aactattata
    13021 cttccagcag ttactaattg accatcgaat ctcttaacac ctaatctttt agatatagaa
    13081 tctctaccat ttttagaaga acctacccct tttttagatg ctaataattg taaattcatg
    13141 tttaacatat ggagtcaccc cctacttttt taaaattttt atattttttg ggtattcccg
    13201 tttaatttct ttaaccgcta attgaaagtg atttagtaat aattgtgcgt cttttacatg
    13261 tgtatctttg actatacact ctatatgacc ttcttctcct actacttttt caaattcaat
    13321 cttctgtaat ttgtctaaag aatttataac tgcaatagca ttagatgtaa ctgcagcaca
    13381 tactatatca tagcctattt cagcaaaatc agcatgtccc tttaaacaaa aaccttgcaa
    13441 caagaaatca tcattataat aatatttaac ttttatcata acttaaccaa taactaagcg
    13501 tttatttttt ctacaactat cttagtgtat gattgacgat gtccttgttt tcttctgtaa
    13561 tctttttttg ctttatactt gaaaactata actttcttag ctttaccttg ttcaacaact
    13621 ttagcttgaa cagatgctcc ttctactact ggagaaccta attttaattc tccatcttta
    13681 gagcaagcta atacttcgtt taaagtaaca acatcacctg cgttagcttc taatttttca
    13741 acgaataata catcaccttc agaaacttta tattgctttc caccagtctt tactatagcg
    13801 tacattaaat acacctcctc ggcctcaaac tcgccagtac aaggtacttc atttgaagat
    13861 ttaaaccttt actgtgcggc attacaatta ttaattataa aatatttaca ttttattgtc
    13921 aactatttta tcgaaaataa cattggtttt attgtgcttt aatgtactat ttgcgtttaa
    13981 agaaatcttt atattatatt tttcaccaat tttatcaatt atatccatat aatattcttt
    14041 tatttgttca aaaataaaat cattaaattc taccattatg tttttgtaaa cagtatgttc
    14101 acttattctc ataatttctt tttcaatact atctagtata taatgtacag attttattgt
    14161 ttgttcacca tcacaacatg gacaagacat taaataatac ttatcaatgg attctttttc
    14221 tctacgccta gcaacttcta ctaaaccaag ttttgtcata cctaatactt cagcttttct
    14281 tttatcttta ttaaattctt cttctaggat gtgtaaaatc tccttttgat gcttcttttt
    14341 atgcatatca ataaaatcga ttattattat cccagctatg tctctaattc taagctgtct
    14401 tacaatttct tttgcagctt caagattagt tttataaacg gtttcatcaa gtttaccagt
    14461 tccaataaac ttacccgtat ttacatctat aaccgtaaga gcttctgttt tttcaattat
    14521 taaataacct ccactcttta accatacttt tttatttaat aatttttcta ttttagattc
    14581 tattctgtat aaatcaaata catctctatt ttcctcaaga cacagtttat caacataagt
    14641 cttatctata cctcttaaaa tagacttaag ttcagagtac ttatcataac tattggtaat
    14701 tatagattca atatcatcat tcacattatc ttttacatat ttagttgcaa aatctaaaga
    14761 cttatataat aatttcggac ctataccaag tttatattct tttaaaatgt tttcatactt
    14821 taatttcaac tcttctatat ctttctttat ttcatcatga gtgcaacctt gagcctcagt
    14881 ccttattatt aatcctagat tttccttatt tactgcctta gtaatttttt ttaatctaaa
    14941 tcgttctttt tcatcagtaa ttttatttga tattgtagtt ctatcattgg aaggtatata
    15001 gacaatatac cttccagaaa tactaatctc agtgtttaat tttgcgcctt tagttcctat
    15061 ttcttcttta tttatctgaa ccaaaatatc ctgaccagat tttatagtac ttcccttttt
    15121 taattgtaaa taagcaagtt tcttaaatcc aatatctaca aaacaagctt ctattcctgg
    15181 tataactttt tttacgattc cacgatatat attggataca gtcttcttat ttaaattgtc
    15241 ttcaacaaaa agttctgtta atctttcatc ttcaagtact gctgttttct gggaccctat
    15301 tagggactca ataactatct ttttcacaac ttcacctctc gagcttacat aggagtcact
    15361 aattccccat cttctaatac atataaatct cttctcaata tatcaacatc taatgggtca
    15421 atatcaagtt caaacatctc taatatcttt ggtataagta tattagtatt taaattagtt
    15481 tttgagcctg tagctatagt agcagtaaga gtaatatgtt catcttctaa atttaaaaca
    15541 tcaaaggttc ttatcatagg tcttatatct acttcaacca tcttaccttt tttattcttt
    15601 tttgttatga ttatttcttt attattcata aagtcaatta cttttctttt tacaaactct
    15661 ttcgtcatag gtcttttcaa atctatatta aacaaatatt ctccatagtc tatcacagaa
    15721 gaaagagaag gtgtttgtct atcaatttct tttgctttga taaaatctat cccatctgga
    15781 agttgaattg atactttatt taaaaattcc tctactgata aatcatcttc ttctatttca
    15841 atatctacat actcaccttg actttctgcc cctaaagcca aagcattgcc ataactcatt
    15901 ttagggtgag gattaaatcc ttgagaatgt gataagttta tttcagctct tctaaacgct
    15961 ctttgtaaca atctctgaag gtcaagatgt gatatatata tcatgtcgcc ttccttttta
    16021 aattttactc ttattatctt actcatatta gcaaagcccc cttcctatat catgtatatt
    16081 tattccacaa ccattgcagt tatgtctaca atcagaagtg attttttctt ttttagcatt
    16141 ttcgtcttct ctaattaaga attttttact aatacctaca tctatatggt cccaaggaaa
    16201 tacctcttca tagtttctat ttctatgtgc ataaaaatct atagaaagat tattcttttc
    16261 catagcttct ttccatatat ctaaattaaa atgttctgcc catccatcaa atttagcacc
    16321 tagtctaaat gcatcatata taacttttcc tattcttcta tcgcctctag ctactactgc
    16381 ttccatcaaa ctagtctttg aatcatgata attgtatttt atattgttat ttcttaattt
    16441 atttactaaa tgtctctgct tattaataac ttcttctgta gtatcttgac catgccattg
    16501 gaaaggtgta aatggctttg gtacaaatgt agaagtactc actgtaacac taaaacttct
    16561 ctttagcttt cctccattta cttttctata aatatcaata actttataag ccaatttagc
    16621 tataccatct aaatcttcat aagtctcagt tggtaaacca atcataaaat acaacttaac
    16681 actattccac cccatctcaa aagctctttc tgttgcattt tccaaatctt cttctgtaac
    16741 acctttattt ataacatctc taagtctttg agttcctgct tctggagcaa aagtaagtcc
    16801 agattttctt acttgttgta ttttatcagc aatttccatt gaaaaattat ctaatctaag
    16861 ggatggaagc gaaatcccaa tattattaga tgcatattca tctaccaaat aatcagtcaa
    16921 ctctgataac ttactataat cactcgtact aagtgatgat aatgatattt catcatatcc
    16981 agtattctta actaatttat ctaatatttc tttaagtctt ttaacactct tttctcttat
    17041 tggcctgtat atcatacctg cttgacagaa tctacatcct ctagtacatc ctctaaataa
    17101 ttctagtact attctatcat gaacagtatc tatataaggt actattagtt tttctggata
    17161 atctacagtc tcaacatctt ttattatcct tttagtagga tttttagtaa tcccttctct
    17221 attaggagtt acactttgaa ctgtaccatc ttcattgtac ttgacatcat aaaaacttgg
    17281 tatgtatact ccttctatgc tagatatttt atataaaaaa tcttctctag tcgttttatt
    17341 tttcttccat tctttatact catttactac ttctaaatta acctcttcac cttcacctaa
    17401 tattactaca tctacaaaat ctgctaatgg ttcggaatta taagcacaag gaccaccaac
    17461 cattataaat gggtcttcca aagttctttc ttcttttaat attggtatat tggctaaatc
    17521 taatatattt aagatattag tgtatgaaag ttcatattga agtgtaaaag tgacaaaatc
    17581 aaagtttgtt ataggctctc ttgactctaa tgcaaataat ggtatacttt tttctctcat
    17641 tatattttcc atatcaactg caggagaata cactctctca caaaatacat cttcatcctt
    17701 atttattaca tcatataata tatgactccc taagtgactc ataccaactt catataaatc
    17761 tggaaagcaa tgtgcatatc ttatcaattc actattacta gtatctttat gaattgaatt
    17821 tatttcattt ccaagatatc tagcaggctt ttctactttt tttaagattt ttttcaagtc
    17881 tactttattc atttattaaa attcctccaa acaagctctt tatttttatt cttatttata
    17941 agtaatatta tacacgataa aatattttct atcaaaaata taactaaaat attgaattta
    18001 aaatacatat tatt
//
LOCUS       DAYASV010000077        17937 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYASV010000077 DAYASV010000000
VERSION     DAYASV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.077"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            438..3983
                     /locus_tag="ACGI68_002452"
     CDS             438..3983
                     /locus_tag="ACGI68_002452"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0138885.1"
                     /translation="MNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHTY
                     LSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRMT
                     ISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKSG
                     KIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQLF
                     DKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKIV
                     SIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGGV
                     KTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQLE
                     KQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSKR
                     FEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFKD
                     DRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVDI
                     TSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENAT
                     KNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDNN
                     GKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKGA
                     LMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDIS
                     ETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALYS
                     AKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIEV
                     AVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLGN
                     YLLNFNADGLFYCNDIENLGSDVKKVLKPQGICSILQCKILEGNEFRGFIGFDECSYN
                     RMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTYE
                     LLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKFE
                     VQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            4168..6102
                     /locus_tag="ACGI68_002453"
     CDS             4168..6102
                     /locus_tag="ACGI68_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFH0138886.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            6208..7203
                     /locus_tag="ACGI68_002454"
     CDS             6208..7203
                     /locus_tag="ACGI68_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HFH0138887.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            7175..7960
                     /locus_tag="ACGI68_002455"
     CDS             7175..7960
                     /locus_tag="ACGI68_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138888.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7948..8646
                     /locus_tag="ACGI68_002456"
     CDS             7948..8646
                     /locus_tag="ACGI68_002456"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138889.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8769..9242
                     /gene="ybaK"
                     /locus_tag="ACGI68_002457"
     CDS             8769..9242
                     /gene="ybaK"
                     /locus_tag="ACGI68_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFH0138890.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKFYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9439..10278
                     /locus_tag="ACGI68_002458"
     CDS             9439..10278
                     /locus_tag="ACGI68_002458"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFH0138891.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKN
                     VVI"
     gene            10291..10839
                     /locus_tag="ACGI68_002459"
     CDS             10291..10839
                     /locus_tag="ACGI68_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HFH0138892.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10905..12086
                     /locus_tag="ACGI68_002460"
     CDS             10905..12086
                     /locus_tag="ACGI68_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HFH0138893.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEIKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12378..12767
                     /locus_tag="ACGI68_002461"
     CDS             12378..12767
                     /locus_tag="ACGI68_002461"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0138894.1"
                     /translation="MMEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNIT
                     AYDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQ
                     PGVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            12917..13180
                     /locus_tag="ACGI68_002462"
     CDS             12917..13180
                     /locus_tag="ACGI68_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0138895.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13309..14151)
                     /locus_tag="ACGI68_002463"
     CDS             complement(13309..14151)
                     /locus_tag="ACGI68_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HFH0138896.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            14446..15180
                     /locus_tag="ACGI68_002464"
     CDS             14446..15180
                     /locus_tag="ACGI68_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0138897.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            15190..16323
                     /gene="nagA"
                     /locus_tag="ACGI68_002465"
     CDS             15190..16323
                     /gene="nagA"
                     /locus_tag="ACGI68_002465"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0138898.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            16422..17171
                     /gene="nagB"
                     /locus_tag="ACGI68_002466"
     CDS             16422..17171
                     /gene="nagB"
                     /locus_tag="ACGI68_002466"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFH0138899.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            complement(17303..17722)
                     /locus_tag="ACGI68_002467"
     CDS             complement(17303..17722)
                     /locus_tag="ACGI68_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138900.1"
                     /translation="MKKALRYLLFLIILLLMYFLAVKLNYDFYTQFYTGYMQDFKRYI
                     FINLISSGGIGLLLGTELLIREYKKDGSWYIDIPRLLLLCFPSFLLSLMPVFFFMFPV
                     GNIPIIGNFIMLDRIPLNIIIFNILFGYFLITSFRKK"
BASE COUNT         7180 a         1879 c         3151 g         5727 t
ORIGIN      
        1 aacttaagtt ttgagtatta atctaaagta tatgttttta tatggtttaa attttatgta
       61 tacaaaatta atttgctaac tgaatcatat aaaaaaaaac aaaatatttt ttagagggaa
      121 tgattagata gaattttttt gttatgaaat taattgaaga gtaattaatt agaaaaggaa
      181 tgaataataa tgaatatatt acattattac tttttgataa atcaaaaata aagttataat
      241 ttttaatatt aattaagaat gatgaaatat gaataaagat tttacaaata aatcttaagc
      301 tatcttattt aattctaata caggaagaag gtaaatgtat attgaaaaaa tcatagaaat
      361 agaagtaaac aatttaaata aatacaaagg tttgcaaaag aagtaatata attatataca
      421 atgaggatgt gagtatgatg aacagactaa agccaagaat tttaggtatt agtatcttgt
      481 ttattattat aattatttta acatgtggag ctgttttcaa gtttaaattt aaattagata
      541 ataaattaaa agaaaccaca catacatatt tgtctgagtt tacagaacac aatataatta
      601 atctcaagac aagacttagt ggtcaatttg acatgcttga atctattgct aacttcatag
      661 gaaacttgaa taatataaat gatgaaatgg tgattgattt aatggattct gatgtaaagc
      721 gaagttcttt aattagaatg actatctcta cattagatgg ggaatgttat tctaatgatg
      781 gaattaccca taatgttcaa gatagagatt attttaaaaa agctaaaaat ggagaaaaga
      841 atatatcaga gcctttaaaa tctattgtta ataataaaga aataatagtt ttatctaccc
      901 cagtttataa aagtgggaaa atagttggtg ttttaagtgg aatttatgat tcaaaaaagt
      961 taaatgagtt attaggatta tctgcatttg atgaaaaagc agatgttttt gttgcaaaga
     1021 aaaatggaga tatagttgct caaaaaacaa ataaaaatag aaagacgcta tcatgtatat
     1081 ttcagctttt tgacaaagat aattgtgatg aagatgattg tctaaaaact ataaaagaaa
     1141 atatgaaaaa tgataagtct ggatatttta attataactt agataatgaa aaatttatgt
     1201 taaactatca gccacttgga gttaatgatt ggtatgtttt ttctgttgtg ccaaataaaa
     1261 tagtatctat tcagtcagag aatatatctt atgatgtata tatttttgca ttagaaatac
     1321 tactagttat attgattttt ataatatata tagcatgtat tataaataat aacaccaaaa
     1381 ttattataaa tgaaaaagaa aaatttaaag tactcactga taatatatta ggaggggtta
     1441 aaacctgttt aaaagatgaa tatatgacta tgacatatgt aagtcagggg tttttagagc
     1501 ttacaggtta ctcttgtgaa gatttaaata aaatatttaa aaatagattt aaagacatga
     1561 tttataaaga tgatttagac aagtttaatt gtattattct aaatcaatta gaaaaacaag
     1621 ataaaattga agttgaatat agaattgtaa gaaaagatgg aagtataatt tggattttgg
     1681 agagaagtaa gtctataaat gataataaag gaagtgagtg tattcaggga gttttaactg
     1741 atataacaga acttaagaaa atacaagaag aattagattc aaaaagattt gaaatacagg
     1801 ctatcaatga tagtattgtt ggtggaatag tgataactga gataaatgat gattttgata
     1861 gtatatattt aaatgaaggt tatttaaata tgattggata tacacgcaaa caactggaag
     1921 atgagctaaa taataaatta atcaaactta tttttaaaga tgatagaaca gaattaatta
     1981 atagtataaa caaacaactc aaattaagtg atacatttgc atctgaacaa agagttgaaa
     2041 aaagggatgg cactattata tggattttat taaaaggaag aaagatgatt tatgataatg
     2101 gaaaatctgt actttgttgt gttattgtag atattacttc tgctaaatta ttagagcaag
     2161 aacttaaaaa gagtgaagaa cgtcatgaaa tcattctaaa gcagacgcaa aatattataa
     2221 tagaatggga tatagaaaaa gataaaatga atatatcacc taattggtat aataagttta
     2281 aatatgaccc tataactgaa aacgcaacaa aaaatataat taaatcagta gatatatatc
     2341 atgatgatat ggataagttt atttcattaa ttaaacttac ttcacaaggt agtgagtatg
     2401 aagagtgtga aattagactt agaactagag atactattta cgcttggttt agtataaagg
     2461 caacaacttt atttgataat aatggtaaac cttataaagt agtaggaagt ttgactgata
     2521 ttgatgaatc aaaacgagaa gctgaaaagt tgaaagaaag ggcagaaaaa gaccctctta
     2581 cagatctgta taataaaaaa gcatcacaat cacttataga ggagtacata acattggata
     2641 aaaaaagaaa aggtgcttta atgattattg atattgatga ctttaaagga ataaatgata
     2701 atttaggaca tttatatggg gatgctgtat taagtgaaat tgcaaatgac ctaataaatc
     2761 tatttagagc aaccgatata gtaggcagaa ttggtggaga tgagtttatt gtatttataa
     2821 aagatatttc tgaaacaaga gatattataa ataaagcaga agaattggta aaaacatttg
     2881 aaaggtcttt tttaggtaaa ggacgaaatt ataaaatatc attaagtgta ggaattgcta
     2941 agtttccaca agatggtatg gactatatga cactatttag aaatgcggat atagctcttt
     3001 actcagccaa agggaagggc aagaattgtt atacattgta tgataaaaaa tttgataaag
     3061 tccaatatat aactaataca agaaatgcag gagatagtga taatgtagag atgtctttta
     3121 aagaacatat cacaaattat atatttgata tactatatga atcaagtgat atagaagtgg
     3181 cagtaaattt aattctaagt attgtaggta aacactttaa tgtgagtcgc atgtatatat
     3241 ttgaaaattc agatgataat cgagtatgta aaaatacatt tgaatggtgt aacgaaggtg
     3301 taacctcgga aattaataat cttaaaaata tttgttacaa ggatttaggc aactatcttt
     3361 taaattttaa tgcagatggg ttattttatt gtaatgatat tgaaaattta ggtagtgatg
     3421 tgaaaaaagt acttaagcca caaggcatat gttcaatatt acaatgtaaa attttagagg
     3481 gtaatgaatt tagagggttc ataggatttg atgaatgttc atataatcgt atgtggacta
     3541 atgatgaagt gaacactttg acatttatat ctaaaacgct cagtatgttt ttattaaaaa
     3601 tgcgtattca gagtaagcta gaagagtctt taacaattca aaaatctgtg atggataata
     3661 tggatatatg ggcatatgtt gttgacagga atacctatga attactattt atcaataaaa
     3721 aaatgcttga gtcagcacca aacactcatg taggtgattt atgttataaa gcgatatgga
     3781 ataatcaaga taaaccttgt aaaaaatgcc ctatgatata tttaaaagat ggtgatgata
     3841 cttataaatc attcatgcat aataaaaagt ttgaggtaca ggcaaatata actacaacta
     3901 ctttgaaatg gtttgatggt aaagatgcat gtttaatatt tggaagtgaa ataactagta
     3961 ttgaagctga agaaaatact taaaatactg tgttatatat atacttaaaa tcgcaatttg
     4021 aatttattag acttaaaatg tctatataaa tcatttaagt tgtggtttta agtatttttt
     4081 atgatataat taagcgttaa gaacaaaatt aaaagatact tttgtataaa aaaatatttg
     4141 ttaaatatat attgagcagg tgataatatg gaaaataagt ttttaccaat aagcaaacaa
     4201 gatatgatag atagaggatg ggaagaatta gattttgttc tagttacagg agatgcttat
     4261 gtggaccatc atagttttgg aacagcaata atatctagag ttttagaaag tgctgggtat
     4321 aaagttggaa taatagctca accagactgg agaactacag atgattttat gaagctagga
     4381 aaacctagac ttgcatttct agttaatgca ggaaatatgg attcaatggt caatcattat
     4441 tctgtaagta aaaagcatag agaaaaggat atgtattctc caggtgggaa aatgggttat
     4501 agaccagata gagctactat agtatattgc aataaaataa gagaagcata cagtgatgtt
     4561 gcaatagtaa taggtggaat tgaagcgagc ttaagaagat ttgcacacta tgattattgg
     4621 agtgataagg ttagaaagtc tatgttaata gatagtggag cagatttatt agtttatggt
     4681 atgagtgaaa agcagattgt agaagtagcc aatgcactta atgatggata tgacccaaaa
     4741 tacattagac atattgatgg gacatgctat atttcagata cattggaaga aatatatgat
     4801 aaatatatat taattccatc atataaagaa atttgtgaag ataaaatgaa gtatgtagaa
     4861 gcttttaaaa ttcaatatga tgagcaagac ccatttagag gtaatataat tgttcaacct
     4921 catggaagta agtatctggt tcaaaataag ccagaaaaac cacttagtag agaagaatta
     4981 gatgaagtat atggattacc atatcagaag acataccatc ctgtatatga aaaatttgga
     5041 ggaataccag ctatagaaga agtaaaattt agtatagtaa gttcaagagg ctgttttggt
     5101 agttgttcat tttgtgcaat aacatttcat caaggtagag cagtgcaaag tagaagtgaa
     5161 aaatcaatta ttgatgaagc aataggaata acaaatttag atgattttaa agggtatata
     5221 catgatgttg gaggtccaac agctaatttt agaagaccag catgtaaaaa acaaattact
     5281 aaaggtgctt gtaaaaatag acaatgctta tctccatcac catgtaaaaa tttagatgca
     5341 gaccacagtg aatacttaca tttacttaga gctgtaagaa aattacctaa aataaaaaag
     5401 gtgtttgtgc gttctggaat tagatacgat tatgtaatgg cagataagaa caataaattt
     5461 cttagagagt tgatagagca tcatgtaagt ggtcagttaa aggtagcccc agaacatata
     5521 tcagaagaag ttttaaagta tatgcaaaaa ccagcaggaa aaacttatga taaatttaga
     5581 cagaagtttt ttgctataaa tgaagaatta ggtaaaaagc aatatttgat accctattta
     5641 atgtctagtc accctggttc taccttaaat agcgctatag agttagcaga gtatttaaga
     5701 gatactcact accaaccaga gcaggttcag gatttttatc caactccagg gactctatct
     5761 acaaccatgt tttatacagg tattgacccg cttacaatga agccagttta tgttcctaaa
     5821 tcaaaacgtg acaaggcaat gcaaagggct ttattacaat atagagcacc tagaaattat
     5881 gatttggtat actctgcgtt agttgaagca ggaagagaag acttaattgg ttttggacat
     5941 agatgtctta taaagccaaa aaatgaaaag ccttatttta atagaaataa ttctaaaaaa
     6001 aatgtatcta aaggaacaaa taaaaacaaa aaaactaata caaataacag aagtaataaa
     6061 aatcaacaaa aaagttcaac taaaaaaaag aaaaaacatt aaagtataaa taaaaatact
     6121 taaaaaagaa ggattattcg ataaattaac agaaattagt ataaataaat ataattattt
     6181 taatgacgtt acctttggga ggaagagatg aaaaagtata tttctataat attattagtg
     6241 gtgttgacta tggttgtttt ggtaggatgt tcaccaggaa aagataaccc taaaaataag
     6301 gagctatcag ttgtcaaaca agtaaaagta gctgttccag atggactacc agcagtggca
     6361 atagctaaac ttgcaaatga aaatccagag attaaagaag gatatgaaac agtgtatagc
     6421 attgaaaaga cacctgaagc gatttccact agagtgatga aaaaagatgc agatatagct
     6481 atagttcctt caaacatggc tgcaatagcg tataataaaa cttcatcata taatattgtt
     6541 ggaactgtag gaatgggttc actttatttg gtgtcaacag aaaatataaa agattactct
     6601 gatttagttg gtaaagaagt tggatgtact ggaaaaggac ttactccaga cataactata
     6661 aaaagtcttt tgttacaaaa ggatataaat tattcagata taaaatttaa ttatgtaaat
     6721 tcagcaagtg aactggtacc attattggct actggtaaag ttaaaacagg tattgttcca
     6781 gaacctgcac ttagtgcttt gatgtctaaa aatccagaca taaaaattat aaaaagttta
     6841 aatgactcat ggaaggaagt aagtggttca aaagatggtt atcctcaatc tactttaatt
     6901 gtaaagtctg aatttttaag agataataaa gattttgttg attcttttgt aggtcaatta
     6961 tcaaatagta tagattgggc aaacaaaaat cctgaagaat taggtgcata ttctgaaaaa
     7021 ataaaaatat ctactgaatc aaagataata ggaaaatctc ttgaaagagc taatttgaaa
     7081 tatattccag ttaaggacat gattaaagat tataaaaatt actatgagaa attagctaat
     7141 tttgatgata agacattagg aggcaaagta ccagatgaag caatatactt tgttgagaaa
     7201 taaaaaaaat aaagaacaat ttatagataa aatggaggta acactttctt gtgttatcct
     7261 cttattttta tggcaaatta ttgctttaaa aattgataat gatatttttt taccaacagt
     7321 aggtcaagtg tttaatagta taaaagagat tatgctaaat tctagtttct atatagatat
     7381 actttactct atggggagat gtttttttag ttttttactg gctatgattt ttgctattat
     7441 actttcaata gtgtcttatt taaatagatt ttttagaaac ttattaaagc caatcaatgc
     7501 tttgacacgt tcaataccaa ctatgatatt ggtagtactt gcacttattt ggtttgaaaa
     7561 ggatagtacc ccttttatag ttggatttac tatagttttt cctatattgt atgataacgt
     7621 tttgggcgct atattgaata tagacaagaa cttattagaa atggctaatg tgtataaaat
     7681 aaggtttata gataaggtgt taaaaattta tttaccagcc ataaaatttc aaattatatc
     7741 tatactagta tcaacatttt ctttaagttt gaaagttgct atagcaggag aggtgtatag
     7801 tcaacctaca tatggtattg gaactatgat acagactgaa aaaattaact ttaacacatc
     7861 tggaatattt gcatggataa ttattgttct tttaatatct gctattttac aaatagctca
     7921 aaagttttta gcaaggagag cttttttatg gaaaagataa agttgagtat tgaaaatata
     7981 aataaaagat atgatagtcg tattatattt agagatttta atattgattt ttatgtaaat
     8041 gaggtaaatt gtatccttgg aaaatctggt tgtggaaaaa ctacactctt gaacataata
     8101 agtgggatta ttaaaaatga caccaacaat ctaaatatca aagaaaattt aaatagagta
     8161 ggaaataagc ttgaagctag ttacatattt caagatgata gacttataga ctggttaaca
     8221 gtagaagaaa atataaagat agttgtaaat aaatactata ataaaactca attaaataaa
     8281 atttgtgatg aatacttgga attggttgga attagtgatt ataaaaaatt ctatcctcaa
     8341 atgcttagtg gaggtattag acaaagagta aatattgcta gagcttttat atatccatca
     8401 aaaaacatta taatggatga accttttaaa tctattgatg caaagaatac acagcttata
     8461 atggataatt ttagaaatat attaagaaaa gaaaagagaa ctgtgttatt tgtaactcat
     8521 aatattgagg aagcattgtt tttagcagat aggatattta ttttaggaga ctctccaatt
     8581 agaataaaaa aaatattaaa aaattctaag gaattggaaa aaaatgaagt tttgaagttg
     8641 atatagagtt ataataatac tgtcgtttta aaatatattt ggttaaaatc tataagacta
     8701 tattaaaata gtacagaata ttcaaaactg tgtataatgt tcgtatttta taaaaaggaa
     8761 gtgaatgtat ggttaagact aatgctatga gaatattaga ttcaaataag attgattata
     8821 aagtaatgtc ttatgaagtg aaaagtgaac atgtggatgg tgttgaggtg gcacatgata
     8881 taggaagaga tgtaaatgaa gtgtataaga cattagtaac tcaaggagtt agtaagaata
     8941 tatatgtata tgtaatacca gtacatgaaa acttagactt aaaaaaggct gcaaaagtag
     9001 ctaaagagaa aagtgtagag atgattcatg taaaagatat aaataagctt acaggttata
     9061 ttagaggcgg atgttctcct ataggtatga aaaagtttta taaaactttt gtaaatgaaa
     9121 gtgcaaagaa tttagataca attattgtga gtgcaggaaa aataggatat caaatagaac
     9181 tatctccatt tgatttacaa agacttataa aagtagaatt tgtagatgta ataaaaaaat
     9241 aagaattaca tataaaatat agagcttata ttctcaataa taggtgttgt aatattaata
     9301 aaatgaataa gaattttaaa ataaatattc agttacttat gtgtaattgg tataaaaata
     9361 ttgagaaatt ttaaaaacaa tggtaaagtg taagtataat cacgaatatt ttttataaag
     9421 taatggaggg atgttcgctt gagaaaaata aaatctgaac aaatcgtcga gcaggtaaaa
     9481 aaactttgta tagaagcgag cctatattta ggagaagatg ttttaagttg tataaaagaa
     9541 aaagcgaaaa gcgaaaagag tgaagttgga aaaaatatat tgaatatatt agttgaaaat
     9601 gctgaaatag ctaaagaaaa aaatatacca atatgccaag atactggtat ggctgtattt
     9661 tttgttgaaa taggacaaga agttttaatt gaaggagaca cacttactga tgctataaat
     9721 gagggagtaa gacaagggta tgaagaaggc tacttaagaa aatctgttgt aagtccaatt
     9781 aacagagtaa atacaaaaga taatacacct gctgttattc attatgatat ggtaaaaggt
     9841 gataaaatta aaatagaatt tgcagctaag ggatttggaa gcgaaaatat gagtaagatg
     9901 aaaatgttaa agccatcaga tggattggaa ggaattaaaa aattcataat agatacagtt
     9961 tcagaagctg gacctaaccc atgccctcct atggtaattg gagttggtat aggtgggact
    10021 gtggacaaat gtgctcaaat agctaaaaag gcacttttta gagaactagg tgaatttaat
    10081 aaggacgaaa atatagcaaa acttgaaagt gaactattaa cagctattaa taaacttggt
    10141 ataggacctc aaggcttagg tggtactaca acagctttgg gattaaacat agagactttt
    10201 cctacacaca tagcaggtct acctgttgtt gtaaatataa actgccatgc atcaagacat
    10261 aagaatgtag taatttagga ggaaaataaa atgataaaaa taactacacc tgtaaatgaa
    10321 attgatatag caaaattaaa ttgtggagat acaattagtt tgagtggtat actttataca
    10381 gcaagagatg ctgctcataa aagattgata gattgtataa ataaaggtga agaattacca
    10441 tttgacgtat atggtcaagg aatatattat gtaggaccta ctcctacaaa gccaggagaa
    10501 gttataggtg ctgcaggtcc aacgacgagt tatagaatgg atgatttgac tatacctctt
    10561 ttagaaagag gtcttagatt gatgataggc aagggaaaaa gaagtgatga agttatagaa
    10621 ggtatgcaaa aatacggagc agtttatctt gctgcaatag gaggagctgg agcgtatatt
    10681 tcaaattcaa taaaatcatg tgaaattatt gcatatgaag atttaggagc tgaagctata
    10741 agaaagattg tagtagaaga ccttaagctt acagtagcaa tagatagcta tggaaataat
    10801 atatatgagc aaggtcgagc aatttatgaa tgtaaataat gtttagaaat aaatatgaat
    10861 atcaaaatta aaaatagttg tgtatatttt aaggaggaaa attgatgtct aaggattatg
    10921 caaaattggc attagaaatg catgaaatca acaaagggaa agtaagtgta gaaagcaagg
    10981 tagaaattaa aactaaggat gatttatcaa cagcatatac accaggggtt gctgaacctt
    11041 gtttaaaaat acatgaaaat caagatgatg tttatagata cacttctaaa gggaatctag
    11101 tagcagtagt tagtgatgga agtgctgtac ttggattagg taacattggt gctgaagcat
    11161 ctatacctgt tatggaaggt aaagctatat tatttaaaca atttgcagat gtagacgctt
    11221 ttccaatatg tttaaagact aatgatgtag atgagatagt taaatctgta gagttgatgg
    11281 agcctgtatt tggaggtata aatttggaag acatatcttc tccaagatgt tttgagatag
    11341 aagagagatt aaaaaaatct ttaagtatac ctgtttttca tgatgaccaa catgggacag
    11401 caataatagt tgcagccgcc ataataaact caataaagtt aattgaaaat aaaaaaatag
    11461 aagatttaga aatagttata aacggagcag gagctgctgg tatagccata gcaaagatac
    11521 tacttaatat gaatgttaaa aatataatct tatgtgatag aagtggagca ttggaagcta
    11581 gtatagaaaa tctaaactat gttcaaaaag aaatgttaaa agtaacaaat ataagaaatg
    11641 aaaaaggacc tttaaaagat attataaaag gaaaagatgt atttattggt gtttcaggtc
    11701 caggagctgt tacaaaggaa atggttgaaa gtatgtctga gaagccaata atattggcaa
    11761 tggcaaatcc tactccagag ataatgccag aagaagcaaa attaggtgga gcatttataa
    11821 tgggaacagg tcgttcagac ttccctaatc aagtcaataa tgtactggct tttcctggaa
    11881 tatttagagg tgcacttgat gttaaggcta aagagataaa tgaagaaatg aagatagctg
    11941 ctgcatatgc aattgctaat acaataagtg atgaagaaat atgcccagag tatatattac
    12001 cagatgtatt taataagaat gtagtaaaaa atgttgctaa ggcagttaaa gaagcagcga
    12061 taaaaactaa agtaaataga atataaattt ttatatgttc atatatcctt atattatcgg
    12121 gtatatgggc atttttatta taggtttata ctcatatatc cttactttta tatacatatc
    12181 tttatgatta aaggtgttag aaaatgtagt aaattttata ctatcagact ctatctttat
    12241 tactttagta gatacatggt tgtaaaggtt tatagaaaaa aatataggaa ggcagatttt
    12301 tattgttgcg tatatatgag aatgatataa tatgaatatt ataaattaat aaatttaaat
    12361 ttatggaggt acaagagatg atggaatata aagttaatga ccaagaactt aatgcttcaa
    12421 tgtttatccc attcgtcaac aggatatggc caggagatta tgatatggat aaaacacaga
    12481 ctgcattatc aaaaactttg aatataactg cttatgatga taaagtgctt gtaggatgtc
    12541 tgcgagttct ttcagatggt tattattttg gaacaattac agaattgctt gttcttccag
    12601 agtatcagaa acaaggcatt ggtagtaagc ttttccagct tgctaaagag aacaccccaa
    12661 ctatgctata ttttggtgca cagccaggag tagaagcgtt ctatgaaaaa aatgggtgcc
    12721 aaagaagtct gcaatcctac ataataaaaa agaaagatag ttattaaagt tccagctttc
    12781 aataatctta aaagtacaat attttagtgg aatcatttca ttttttatta ctaatatgac
    12841 ataaacatgt catattagta ttgatatact tttaatatgg ttaagagcca aagaaaaata
    12901 taaggagtga tttaaaatgg aagaaactaa attttgccaa tgctgtgcaa tgccaatggg
    12961 acaaactgat gaattatatg ggacaaataa agatggaagt aaaagtactg attattgtag
    13021 ttattgtttt aaagatggtg agtttacagc tgatataagt atggaagaga tgatagaagt
    13081 ttgtatccct catatgcttc aaagtaataa ggatatgact gaagatgaag caagaacaat
    13141 gatgaacaat ttctttccaa cattaaaacg ctggaaataa ttttatgcaa aattagttat
    13201 atgcaaaaca tgaaatatgg tgttgagaat aaggggtggt gtgctttata gcataccacc
    13261 tgttttgtta cttggagttc agttattttc ctaaatattt taaatatact attttagata
    13321 agttccctct tcatcttgat aaattaatcc ttttttcata agcactccaa gagctcgttt
    13381 aaaatttttc ttacttgaat taaataatat agaaatatct ttagggtcag atttatcatt
    13441 aaatctcata tatccatcag aaccttctaa ataacttaaa atcttatttt ctaaagtatc
    13501 aagttcatcc tttctatttc ctctaggaga aagaccaagt tttccatctt catatatttt
    13561 tatgacatgt aaattttcaa gaacatcacc agtttttagt tcagtaaagt actcattatg
    13621 aagtattaca ccagcatatt tattgtctac acatatcatt gcagaattat tagtttggaa
    13681 tccatataca actccagtta caatatctcc tacattgtaa gtatggtctg ttaataagta
    13741 tgaatctatg tctgtagtag cagcaattct tttgcttttg tctaaatata tataaaataa
    13801 atatttttcg tctctaaata gtggataagt cttagcttta aaaggaacta atatgtcttt
    13861 aggaagtccc atatctataa aagtaccaat gtctgtatta tcaactactt ttaaatgtgc
    13921 tacatcccca accttagcta aaggcggtat taaagttgca gcagttctgt catcagaatc
    13981 tttaaatatg aaagcattta cttcatcacc tatgtttatt tcattttttc cgattaatct
    14041 attatgaaga agtatatcgt cttttgtgtt attagtttgt ccatccaaaa aatatccaaa
    14101 ttcagttttt ctttttactg taagtttatt aaaatcacct atgtttatca aattaaaatc
    14161 atcctctcat tttatattta taatttagcc ataatatact tatgtagtag tttgtcacag
    14221 tatccatatt agtattagtg aaatttacaa taaataatat agttatttat gtatttttca
    14281 attctctagc ttcttatata gtataaactc caatttcttt tttgtcttta ggttttatta
    14341 aaatttttac aaaataagtt aaaaagtatt gaaataaaat ttcttttaaa gtataattgg
    14401 tatataccaa aattggtata taccatatcg aaaagtggtg actatatgaa aatagtaaat
    14461 aaatctagta atataccatt gcatactcaa ttaagttcaa taataagaga gatgatagaa
    14521 actggtgagc ttaaagaggg cgatgcaata atgccagaaa gagaactttg caacatacaa
    14581 aatgtaagtc gtatgacagt aaataaaacc attgttggat tagttacaga aggacttctt
    14641 tatagggtac aaggtaaagg gacattcgtt gcaaagcaaa aaaagaagta tcagttttca
    14701 aatgtaaaag gttttactga tgttatgaaa gaaaagggag taaacataaa aacggatata
    14761 ttatcctttg agatggaact accagatgat ttagttaaaa gaaagcttgg aataagtgat
    14821 aatactacaa atatatataa gattgtcaga cttagatata ctgatggaga gccatttgga
    14881 ttagaaatag tatatctatc agaagaaatg tgtaagggtc taacaaaagg gatattggat
    14941 aattcttcat tatatagggt tttaaatgaa aaatatggct ataagataca aaaagctgaa
    15001 caggtaatgg agccagtaat actttcagat gaagaaagta agcttcttga aacagatgaa
    15061 ggtgctttag ctcttaaact tcatagaaat tcctataata gagaaggtag ccctatagaa
    15121 tataccatat caatatttag aacagataaa tatcaatatg aaatagtatt gagtgagtag
    15181 gtgaataata tgaaatgttt aattaatgga aagataatat taaaaaatca aatcttagaa
    15241 aataaagtat tagtatttga tgaaaaaata attgatatag ctgatagtgt accaaaagat
    15301 tgtgaagtta tagatgctga tggaaaatat atatctccag ggcttattga tattcatata
    15361 catggaaaca tgggaaaaga tacaatggat tcgactgatg aatctattga aacaatttca
    15421 aagtcaatta tgagacatgg agtaacatct tttcttccaa ctacaatgac tatggataaa
    15481 gaacatgttt atgatgcact tgaagttatt aaaaaagccc aaaatagaaa gcttgaaggg
    15541 gcacaagtat taggagcaca tttagaagga ccttttataa atgagaatta taagggagca
    15601 caaaatgaaa aatttataat aaattcaaaa tacgaattta taaaagaata taaagatgtt
    15661 ataaaagtta taacttatgc tccagaaaaa gatattgatt ttgattttac tagagaaata
    15721 aaaagatgta cagatatagt tttatctatt ggtcatagta atgcaaatta tgaccaagct
    15781 aaagaagcta taaatttagg agtaactaat gtaactcaca tgttcaatgc aatgactggt
    15841 ttaaaccata gagacccagg cgttgttggt gcagcactta caactaatgt gtacagtgag
    15901 ttaatagcag atacaatcca tataaataaa gatttatttc aatttatact aaataataaa
    15961 ggaaaagaaa gactgatttt aataacagat tctatagaag ctggtggtct tgaagatggc
    16021 aattattctc ttggaggtca gaaagtcata gttaagggta atgaagctag acttgaaaat
    16081 ggagcattag ctggaagtgt gttgtcttta aataaaatgg tatttaactt cttagataat
    16141 acaaacctta aagtgaatga agcaattaat ttagcttctt taaatccagc aacatctttg
    16201 ggaataaatg acaaaaaagg aagcttagaa ataggtaagg atgcagatat agctgtattt
    16261 gatgagaatt tagattgtaa aatgacactt tgcttggggg acgtagtata taaaaatata
    16321 tagtatacaa aacaaacctt aaagacagta aaaacaatat aaaaaataat aaattaaact
    16381 tacattagta aaagtagtag ttaaaattta ggaggaataa gatgagagta ttagtgtgta
    16441 aagattatga tggaatgagt aaaaaagccg ctgaaatgat agcagctcaa atagttttaa
    16501 aaccaaattc aatactggga ctagctacag gaagtacacc agtaggtatg tatagagatt
    16561 tagtaaaaaa atacaatgat aatatagtag atttttcaga tgtgatgagc tttaatttag
    16621 atgaatatta taagttacca atatctaatg accaaagcta tgattatttt atgaaagaaa
    16681 atctatttaa tcatgtaaac ataaagccag aaaatactca tctaccaaat ggaatggctg
    16741 atgatattga aaaagagtgt atgaactatg aagcttctat agatgcagct ggtggtatag
    16801 atgtacaagt acttggaata ggtcgtaatg ctcatatagg ttttaatgaa cctgatacta
    16861 aatttgcaaa gagaactcat gtagttgaac ttacagagtc tacaatagaa gctaatgcaa
    16921 gattctttaa atcaagagaa gatgttccta aaaaagctgt aagtatggga ataggttcaa
    16981 tattgaagtc taagaaaata ttgcttttag cttcaggaga agaaaaagct gatgcagtat
    17041 ataatactgt ttatggagat ataactccag aagttcctgg ttctatactt caacttcata
    17101 aagacactat agttatagta gatgaagcag cagcaagtaa attgaatcca aaagattata
    17161 aattagtata agaatagaat gtagtttaag taaaaaatag ctatcattga gtaatcaaat
    17221 ttatttaatg atagctattt ttatttaaat tttttacttc tttttattta aattagttat
    17281 tattgtttaa attacttgct tattactttt tacgaaaact cgttatcaag aagtatccaa
    17341 ataagatgtt aaaaattatt atgtttagtg ggattctatc aagcataata aaattaccta
    17401 taattgggat attaccaact ggaaacataa agaagaatac aggcatcagt gatagtaaaa
    17461 aagatggaaa acaaagaaga agtaatcttg gaatatcaat ataccaacta ccatcctttt
    17521 tgtattctct aatcaataat tcagttccta gtaataaacc aataccacca gaggaaatta
    17581 aattaataaa aatgtatctt ttaaagtctt gcatataacc agtgtaaaat tgagtataga
    17641 aatcataatt taatttaaca gctaaaaaat acataagcaa tagaatgatt aaaaataaaa
    17701 gatatctaag agcttttttc attttaaatt ctcccttcag aaatacttta ttcagtttta
    17761 ttttgttgga atctgatttt atcatttagt ttaagaaatc cataatatta gtgaagaatg
    17821 aaataagtat tatatatgaa taaatatttt tagaattaag gtgaattgta gtgcttaata
    17881 taactataat attaattgtt tgaatattca ataaaaagtt tgaaaaatat caatttt
//
LOCUS       DAYASV010000078        17885 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYASV010000078 DAYASV010000000
VERSION     DAYASV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17885
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.078"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            222..7322
                     /gene="tcdB"
                     /locus_tag="ACGI68_002468"
                     /gene_synonym="toxB"
     CDS             222..7322
                     /gene="tcdB"
                     /locus_tag="ACGI68_002468"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Toxigenic strains produce this toxin (TcdB) or
                     TcdA. TcdB-producing strains are tumorigenic in mice;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HFH0138901.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            7444..7944
                     /gene="tcdE"
                     /locus_tag="ACGI68_002469"
     CDS             7444..7944
                     /gene="tcdE"
                     /locus_tag="ACGI68_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HFH0138902.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8023..8184
                     /locus_tag="ACGI68_002470"
     CDS             8023..8184
                     /locus_tag="ACGI68_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138903.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            8249..8455
                     /locus_tag="ACGI68_002471"
     CDS             8249..8455
                     /locus_tag="ACGI68_002471"
                     /inference="COORDINATES: protein motif:HMM:NF022413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BhlA/UviB family holin-like peptide"
                     /protein_id="HFH0138904.1"
                     /translation="MKLFKNTKIRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKT
                     LKKYKICLQITIRQSPYLIEESIN"
     gene            8672..16804
                     /gene="tcdA"
                     /locus_tag="ACGI68_002472"
                     /gene_synonym="toxA"
     CDS             8672..16804
                     /gene="tcdA"
                     /locus_tag="ACGI68_002472"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HFH0138905.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17139..17837)
                     /gene="tcdC"
                     /locus_tag="ACGI68_002473"
     CDS             complement(17139..17837)
                     /gene="tcdC"
                     /locus_tag="ACGI68_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HFH0138906.1"
                     /translation="MFSKKNEGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
BASE COUNT         7040 a         2006 c         2799 g         6040 t
ORIGIN      
        1 atattttata tagaacaatg tttacatatt tatttcagac aacgtcttta ttcaatcgaa
       61 gagcaaatta atcaactgag tgtcttaaat ttaaaatgtt aggaagtgaa tgtatatgaa
      121 aacctaagta gatattagta tattttataa atagaaagga ggatatataa aagagtttta
      181 gcatttagat gtaaaaatat tatagtaaag gagaaaattt tatgagttta gttaatagaa
      241 aacagttaga aaaaatggca aatgtaagat ttcgtactca agaagatgaa tatgttgcaa
      301 tattggatgc tttagaagaa tatcataata tgtcagagaa tactgtagtc gaaaaatatt
      361 taaaattaaa agatataaat agtttaacag atatttatat agatacatat aaaaaatctg
      421 gtagaaataa agccttaaaa aaatttaagg aatatctagt tacagaagta ttagagctaa
      481 agaataataa tttaactcca gttgagaaaa atttacattt tgtttggatt ggaggtcaaa
      541 taaatgacac tgctattaat tatataaatc aatggaaaga tgtaaatagt gattataatg
      601 ttaatgtttt ttatgatagt aatgcatttt tgataaacac attgaaaaaa actgtagtag
      661 aatcagcaat aaatgataca cttgaatcat ttagagaaaa cttaaatgac cctagatttg
      721 actataataa attcttcaga aaacgtatgg aaataattta tgataaacag aaaaatttca
      781 taaactacta taaagctcaa agagaagaaa atcctgaact tataattgat gatattgtaa
      841 agacatatct ttcaaatgag tattcaaagg agatagatga acttaatacc tatattgaag
      901 aatccttaaa taaaattaca cagaatagtg gaaatgatgt tagaaacttt gaagaattta
      961 aaaatggaga gtcattcaac ttatatgaac aagagttggt agaaaggtgg aatttagctg
     1021 ctgcttctga catattaaga atatctgcat taaaagaaat tggtggtatg tatttagatg
     1081 ttgatatgtt accaggaata caaccagact tatttgagtc tatagagaaa cctagttcag
     1141 taacagtgga tttttgggaa atgacaaagt tagaagctat aatgaaatac aaagaatata
     1201 taccagaata tacctcagaa cattttgaca tgttagacga agaagttcaa agtagttttg
     1261 aatctgttct agcttctaag tcagataaat cagaaatatt ctcatcactt ggtgatatgg
     1321 aggcatcacc actagaagtt aaaattgcat ttaatagtaa gggtattata aatcaagggc
     1381 taatttctgt gaaagactca tattgtagca atttaatagt aaaacaaatc gagaatagat
     1441 ataaaatatt gaataatagt ttaaatccag ctattagcga ggataatgat tttaatacta
     1501 caacgaatac ctttattgat agtataatgg ctgaagctaa tgcagataat ggtagattta
     1561 tgatggaact aggaaagtat ttaagagttg gtttcttccc agatgttaaa actactatta
     1621 acttaagtgg ccctgaagca tatgcggcag cttatcaaga tttattaatg tttaaagaag
     1681 gcagtatgaa tatccatttg atagaagctg atttaagaaa ctttgaaatc tctaaaacta
     1741 atatttctca atcaactgaa caagaaatgg ctagcttatg gtcatttgac gatgcaagag
     1801 ctaaagctca atttgaagaa tataaaagga attattttga aggttctctt ggtgaagatg
     1861 ataatcttga tttttctcaa aatatagtag ttgacaagga gtatctttta gaaaaaatat
     1921 cttcattagc aagaagttca gagagaggat atatacacta tattgttcag ttacaaggag
     1981 ataaaattag ttatgaagca gcatgtaact tatttgcaaa gactccttat gatagtgtac
     2041 tatttcagaa aaatatagaa gattcagaaa ttgcatatta ttataatcct ggagatggtg
     2101 aaatacaaga aatagacaag tataaaattc caagtataat ttctgataga cctaagatta
     2161 aattaacatt tattggtcat ggtaaagatg aatttaatac tgatatattt gcaggttttg
     2221 atgtagattc attatccaca gaaatagaag cagcaataga tttagctaaa gaggatattt
     2281 ctcctaagtc aatagaaata aatttattag gatgtaatat gtttagctac tctatcaacg
     2341 tagaggagac ttatcctgga aaattattac ttaaagttaa agataaaata tcagaattaa
     2401 tgccatctat aagtcaagac tctattatag taagtgcaaa tcaatatgaa gttagaataa
     2461 atagtgaagg aagaagagaa ttattggatc attctggtga atggataaat aaagaagaaa
     2521 gtattataaa ggatatttca tcaaaagaat atatatcatt taatcctaaa gaaaataaaa
     2581 ttacagtaaa atctaaaaat ttacctgagc tatctacatt attacaagaa attagaaata
     2641 attctaattc aagtgatatt gaactagaag aaaaagtaat gttaacagaa tgtgagataa
     2701 atgttatttc aaatatagat acgcaaattg ttgaggaaag gattgaagaa gctaagaatt
     2761 taacttctga ctctattaat tatataaaag atgaatttaa actaatagaa tctatttctg
     2821 atgcactatg tgacttaaaa caacagaatg aattagaaga ttctcatttt atatcttttg
     2881 aggacatatc agagactgat gagggattta gtataagatt tattaataaa gaaactggag
     2941 aatctatatt tgtagaaact gaaaaaacaa tattctctga atatgctaat catataactg
     3001 aagagatttc taagataaaa ggtactatat ttgatactgt aaatggtaag ttagtaaaaa
     3061 aagtaaattt agatactaca cacgaagtaa atactttaaa tgctgcattt tttatacaat
     3121 cattaataga atataatagt tctaaagaat ctcttagtaa tttaagtgta gcaatgaaag
     3181 tccaagttta cgctcaatta tttagtactg gtttaaatac tattacagat gcagccaaag
     3241 ttgttgaatt agtatcaact gcattagatg aaactataga cttacttcct acattatctg
     3301 aaggattacc tataattgca actattatag atggtgtaag tttaggtgca gcaatcaaag
     3361 agctaagtga aacgagtgac ccattattaa gacaagaaat agaagctaag ataggtataa
     3421 tggcagtaaa tttaacaaca gctacaactg caatcattac ttcatctttg ggggtagcta
     3481 gtggatttag tatactttta gttcctttag caggaatttc agcaggtata ccaagcttag
     3541 taaacaatga acttgtactt cgagataagg caacaaaggt tgtagattat tttaaacatg
     3601 tttcattagt tgaaactgaa ggagtattta ctttattaga tgataaaata atgatgccac
     3661 aagatgattt agtgatatca gaaatagatt ttaataataa ttcaatagtt ttaggtaaat
     3721 gtgaaatctg gagaatggaa ggtggttcag gtcatactgt aactgatgat atagatcact
     3781 tcttttcagc accatcaata acatatagag agccacactt atctatatat gacgtattgg
     3841 aagtacaaaa agaagaactt gatttgtcaa aagatttaat ggtattacct aatgctccaa
     3901 atagagtatt tgcttgggaa acaggatgga caccaggttt aagaagctta gaaaatgatg
     3961 gcacaaaact gttagaccgt ataagagata actatgaagg tgagttttat tggagatatt
     4021 ttgcttttat agctgatgct ttaataacaa cattaaaacc aagatatgaa gatactaata
     4081 taagaataaa tttagatagt aatactagaa gttttatagt tccaataata actacagaat
     4141 atataagaga aaaattatca tattctttct atggttcagg aggaacttat gcattgtctc
     4201 tttctcaata taatatgggt ataaatatag aattaagtga aagtgatgtt tggattatag
     4261 atgttgataa tgttgtgaga gatgtaacta tagaatctga taaaattaaa aaaggtgatt
     4321 taatagaagg tattttatct acactaagta ttgaagagaa taaaattatc ttaaatagcc
     4381 atgagattaa tttttctggt gaggtaaatg gaagtaatgg atttgtttct ttaacatttt
     4441 caattttaga aggaataaat gcaattatag aagttgattt attatctaaa tcatataaat
     4501 tacttatttc tggcgaatta aaaatattga tgttaaattc aaatcatatt caacagaaaa
     4561 tagattatat aggattcaat agcgaattac agaaaaatat accatatagc tttgtagata
     4621 gtgaaggaaa agagaatggt tttattaatg gttcaacaaa agaaggttta tttgtatctg
     4681 aattacctga tgtagttctt ataagtaagg tttatatgga tgatagtaag ccttcatttg
     4741 gatattatag taataatttg aaagatgtca aagttataac taaagataat gttaatatat
     4801 taacaggtta ttatcttaag gatgatataa aaatctctct ttctttgact ctacaagatg
     4861 aaaaaactat aaagttaaat agtgtgcatt tagatgaaag tggagtagct gagattttga
     4921 agttcatgaa tagaaaaggt aatacaaata cttcagattc tttaatgagc tttttagaaa
     4981 gtatgaatat aaaaagtatt ttcgttaatt tcttacaatc taatattaag tttatattag
     5041 atgctaattt tataataagt ggtactactt ctattggcca atttgagttt atttgtgatg
     5101 aaaatgataa tatacaacca tatttcatta agtttaatac actagaaact aattatactt
     5161 tatatgtagg aaatagacaa aatatgatag tggaaccaaa ttatgattta gatgattctg
     5221 gagatatatc ttcaactgtt atcaatttct ctcaaaagta tctttatgga atagacagtt
     5281 gtgttaataa agttgtaatt tcaccaaata tttatacaga tgaaataaat ataacgcctg
     5341 tatatgaaac aaataatact tatccagaag ttattgtatt agatgcaaat tatataaatg
     5401 aaaaaataaa tgttaatatc aatgatctat ctatacgata tgtatggagt aatgatggta
     5461 atgattttat tcttatgtca actagtgaag aaaataaggt gtcacaagtt aaaataagat
     5521 tcgttaatgt ttttaaagat aagactttgg caaataagct atcttttaac tttagtgata
     5581 aacaagatgt acctgtaagt gaaataatct tatcatttac accttcatat tatgaggatg
     5641 gattgattgg ctatgatttg ggtctagttt ctttatataa tgagaaattt tatattaata
     5701 actttggaat gatggtatct ggattaatat atattaatga ttcattatat tattttaaac
     5761 caccagtaaa taatttgata actggatttg tgactgtagg cgatgataaa tactacttta
     5821 atccaattaa tggtggagct gcttcaattg gagagacaat aattgatgac aaaaattatt
     5881 atttcaacca aagtggagtg ttacaaacag gtgtatttag tacagaagat ggatttaaat
     5941 attttgcccc agctaataca cttgatgaaa acctagaagg agaagcaatt gattttactg
     6001 gaaaattaat tattgacgaa aatatttatt attttgatga taattataga ggagctgtag
     6061 aatggaaaga attagatggt gaaatgcact attttagccc agaaacaggt aaagctttta
     6121 aaggtctaaa tcaaataggt gattataaat actatttcaa ttctgatgga gttatgcaaa
     6181 aaggatttgt tagtataaat gataataaac actattttga tgattctggt gttatgaaag
     6241 taggttacac tgaaatagat ggcaagcatt tctactttgc tgaaaacgga gaaatgcaaa
     6301 taggagtatt taatacagaa gatggattta aatattttgc tcatcataat gaagatttag
     6361 gaaatgaaga aggtgaagaa atctcatatt ctggtatatt aaatttcaat aataaaattt
     6421 actattttga tgattcattt acagctgtag ttggatggaa agatttagag gatggttcaa
     6481 agtattattt tgatgaagat acagcagaag catatatagg tttgtcatta ataaatgatg
     6541 gtcaatatta ttttaatgat gatggaatta tgcaagttgg atttgtcact ataaatgata
     6601 aagtcttcta cttctctgac tctggaatta tagaatctgg agtacaaaac atagatgaca
     6661 attatttcta tatagatgat aatggtatag ttcaaattgg tgtatttgat acttcagatg
     6721 gatataaata ttttgcacct gctaatactg taaatgataa tatttacgga caagcagttg
     6781 aatatagtgg tttagttaga gttggggaag atgtatatta ttttggagaa acatatacaa
     6841 ttgagactgg atggatatat gatatggaaa atgaaagtga taaatattat ttcaatccag
     6901 aaactaaaaa agcatgcaaa ggtattaatt taattgatga tataaaatat tattttgatg
     6961 agaagggcat aatgagaacg ggtcttatat catttgaaaa taataattat tactttaatg
     7021 agaatggtga aatgcaattt ggttatataa atatagaaga taagatgttc tattttggtg
     7081 aagatggtgt catgcagatt ggagtattta atacaccaga tggatttaaa tactttgcac
     7141 atcaaaatac tttggatgag aattttgagg gagaatcaat aaactatact ggttggttag
     7201 atttagatga aaagagatat tattttacag atgaatatat tgcagcaact ggttcagtta
     7261 ttattgatgg tgaggagtat tattttgatc ctgatacagc tcaattagtg attagtgaat
     7321 agataaaaat atgttaaata tatcctctta tacttaaata tataaaaata aacaaaatga
     7381 tacactacat aaagtgttct atctaatatg aagatttacc aataaaaagg tggactatga
     7441 tgaatgcaca gtagttcacc tttttatatt tctaatggta acaaaatatt tttttatata
     7501 aacctaggag gcgttatgaa tatgacaata tcttttttat cagagcatat atttataaag
     7561 ttagtaattt taactatatc atttgataca ttattaggat gtttaagtgc aataaaaagt
     7621 cgtaaattta attctagttt tggaatagat ggaggaatca gaaaagtagc aatgatagca
     7681 tgtatatttt ttttatcagt agttgacatt cttacaaagt ttaacttttt atttatgtta
     7741 ccacaagatt gtatcaattt tttaagacta aaacatcttg gaatatctga atttttctct
     7801 attttattta ttttatatga aagtgtaagt atattaaaaa atatgtgctt atgtggatta
     7861 ccagtaccta agagattaaa ggaaaaaata gcaattttac tagatgcaat gacagatgaa
     7921 atgaatgcta aggatgaaaa gtaagtaatg gtagatataa taaagatatt aacaaataaa
     7981 aagtgttatc caaataagaa tagctgaaag ttatcataat tcatgaaact aataatgaaa
     8041 acgagggagc agatgccaag agacacacaa gtattaaata catataattt cgaagcaagt
     8101 gttcattact atatagatga caaggtagta tatcaaacat tggttcacaa agatggtgca
     8161 tggtcagttg gtaaaatcta ttaagctaca ttagttacag atatcacaaa ctataatagt
     8221 taaacataga aatatgtgta aattgtgatt gaaattattc aaaaacacaa aaatacgtga
     8281 tgaaggacaa aatgatatag aaaataagta tcaaacctta ataaatgatt taattgatag
     8341 tttaaaagtt ataggaaaaa tatataaaga aataaaaaca ttaaaaaaat ataagatatg
     8401 tttacaaatt actatcagac aatctcctta tctaatagaa gagtcaatta actaattgag
     8461 tatctttaaa ttgaaatgtt aggaagtgat ttaaatatga aaacttaaat tataaaaaat
     8521 caatattaat ttatttttaa aaaatagaaa ggagtgtata agatttattt tcaaagttta
     8581 aaaacaagaa aatcaattta aatttcagaa ggaataaatg tggttataga agtggattta
     8641 ttatcaaaaa taataatact aggaggtttt tatgtcttta atatctaaag aagagttaat
     8701 aaaactcgca tatagcatta gaccaagaga aaatgagtat aaaactatac taactaattt
     8761 agacgaatat aataagttaa ctacaaacaa taatgaaaat aaatatttac aattaaaaaa
     8821 actaaatgaa tcaattgatg tttttatgaa taaatataaa acttcaagca gaaatagagc
     8881 actctctaat ctaaaaaaag atatattaaa agaagtaatt cttattaaaa attccaatac
     8941 aagccctgta gaaaaaaatt tacattttgt atggataggt ggagaagtca gtgatattgc
     9001 tcttgaatac ataaaacaat gggctgatat taatgcagaa tataatatta aactgtggta
     9061 tgatagtgaa gcattcttag taaatacact aaaaaaggct atagttgaat cttctaccac
     9121 tgaagcatta cagctactag aggaagagat tcaaaatcct caatttgata atatgaaatt
     9181 ttacaaaaaa aggatggaat ttatatatga tagacaaaaa aggtttataa attattataa
     9241 atctcaaatc aataaaccta cagtacctac aatagatgat attataaagt ctcatctagt
     9301 atctgaatat aatagagatg aaactgtatt agaatcatat agaacaaatt ctttgagaaa
     9361 aataaatagt aatcatggga tagatatcag ggctaatagt ttgtttacag aacaagagtt
     9421 attaaatatt tatagtcagg agttgttaaa tcgtggaaat ttagctgcag catctgacat
     9481 agtaagatta ttagccctaa aaaattttgg cggagtatat ttagatgttg atatgcttcc
     9541 aggtattcac tctgatttat ttaaaacaat atctagacct agctctattg gactagaccg
     9601 ttgggaaatg ataaaattag aggctattat gaagtataaa aaatatataa ataattatac
     9661 atcagaaaac tttgataaac ttgatcaaca attaaaagat aattttaaac tcattataga
     9721 aagtaaaagt gaaaaatctg agatattttc taaattagaa aatttaaatg tatctgatct
     9781 tgaaattaaa atagctttcg ctttaggcag tgttataaat caagccttga tatcaaaaca
     9841 aggttcatat cttactaacc tagtaataga acaagtaaaa aatagatatc aatttttaaa
     9901 ccaacacctt aacccagcca tagagtctga taataacttc acagatacta ctaaaatttt
     9961 tcatgattca ttatttaatt cagctaccgc agaaaactct atgtttttaa caaaaatagc
    10021 accatactta caagtaggtt ttatgccaga agctcgctcc acaataagtt taagtggtcc
    10081 aggagcttat gcgtcagctt actatgattt cataaattta caagaaaata ctatagaaaa
    10141 aactttaaaa gcatcagatt taatagaatt taaattccca gaaaataatc tatctcaatt
    10201 gacagaacaa gaaataaata gtctatggag ctttgatcaa gcaagtgcaa aatatcaatt
    10261 tgagaaatat gtaagagatt atactggtgg atctctttct gaagacaatg gggtagactt
    10321 taataaaaat actgccctcg acaaaaacta tttattaaat aataaaattc catcaaacaa
    10381 tgtagaagaa gctggaagta aaaattatgt tcattatatc atacagttac aaggagatga
    10441 tataagttat gaagcaacat gcaatttatt ttctaaaaat cctaaaaata gtattattat
    10501 acaacgaaat atgaatgaaa gtgcaaaaag ctacttttta agtgatgatg gagaatctat
    10561 tttagaatta aataaatata ggatacctga aagattaaaa aataaggaaa aagtaaaagt
    10621 aacctttatt ggacatggta aagatgaatt caacacaagc gaatttgcta gattaagtgt
    10681 agattcactt tccaatgaga taagttcatt tttagatacc ataaaattag atatatcacc
    10741 taaaaatgta gaagtaaact tacttggatg taatatgttt agttatgatt ttaatgttga
    10801 agaaacttat cctgggaagt tgctattaag tattatggac aaaattactt ccactttacc
    10861 tgatgtaaat aaaaattcta ttactatagg agcaaatcaa tatgaagtaa gaattaatag
    10921 tgagggaaga aaagaacttc tggctcactc aggtaaatgg ataaataaag aagaagctat
    10981 tatgagcgat ttatctagta aagaatacat tttttttgat tctatagata ataagctaaa
    11041 agcaaagtcc aagaatattc caggattagc atcaatatca gaagatataa aaacattatt
    11101 acttgatgca agtgttagtc ctgatacaaa atttatttta aataatctta agcttaatat
    11161 tgaatcttct attggtgatt acatttatta tgaaaaatta gagcctgtta aaaatataat
    11221 tcacaattct atagatgatt taatagatga gttcaatcta cttgaaaatg tatctgatga
    11281 attatatgaa ttaaaaaaat taaataatct agatgagaag tatttaatat cttttgaaga
    11341 tatctcaaaa aataattcaa cttactctgt aagatttatt aacaaaagta atggtgagtc
    11401 agtttatgta gaaacagaaa aagaaatttt ttcaaaatat agcgaacata ttacaaaaga
    11461 aataagtact ataaagaata gtataattac agatgttaat ggtaatttat tggataatat
    11521 acagttagat catacttctc aagttaatac attaaacgca gcattcttta ttcaatcatt
    11581 aatagattat agtagcaata aagatgtact gaatgattta agtacctcag ttaaggttca
    11641 actttatgct caactattta gtacaggttt aaatactata tatgactcta tccaattagt
    11701 aaatttaata tcaaatgcag taaatgatac tataaatgta ctacctacaa taacagaggg
    11761 gatacctatt gtatctacta tattagacgg aataaactta ggtgcagcaa ttaaggaatt
    11821 actagacgaa catgacccat tactaaaaaa agaattagaa gctaaggtgg gtgttttagc
    11881 aataaatatg tcattatcta tagctgcaac tgtagcttca attgttggaa taggtgctga
    11941 agttactatt ttcttattac ctatagctgg tatatctgca ggaatacctt cattagttaa
    12001 taatgaatta atattgcatg ataaggcaac ttcagtggta aactatttta atcatttgtc
    12061 tgaatctaaa aaatatggcc ctcttaaaac agaagatgat aaaattttag ttcctattga
    12121 tgatttagta atatcagaaa tagattttaa taataattcg ataaaactag gaacatgtaa
    12181 tatattagca atggaggggg gatcaggaca cacagtgact ggtaatatag atcacttttt
    12241 ctcatctcca tctataagtt ctcatattcc ttcattatca atttattctg caataggtat
    12301 agaaacagaa aatctagatt tttcaaaaaa aataatgatg ttacctaatg ctccttcaag
    12361 agtgttttgg tgggaaactg gagcagttcc aggtttaaga tcattggaaa atgacggaac
    12421 tagattactt gattcaataa gagatttata cccaggtaaa ttttactgga gattctatgc
    12481 ttttttcgat tatgcaataa ctacattaaa accagtttat gaagacacta atattaaaat
    12541 taaactagat aaagatacta gaaacttcat aatgccaact ataactacta acgaaattag
    12601 aaacaaatta tcttattcat ttgatggagc aggaggaact tactctttat tattatcttc
    12661 atatccaata tcaacgaata taaatttatc taaagatgat ttatggatat ttaatattga
    12721 taatgaagta agagaaatat ctatagaaaa tggtactatt aaaaaaggaa agttaataaa
    12781 agatgtttta agtaaaattg atataaataa aaataaactt attataggca atcaaacaat
    12841 agatttttca ggcgatatag ataataaaga tagatatata ttcttgactt gtgagttaga
    12901 tgataaaatt agtttaataa tagaaataaa tcttgttgca aaatcttata gtttgttatt
    12961 gtctggggat aaaaattatt tgatatccaa tttatctaat actattgaga aaatcaatac
    13021 tttaggccta gatagtaaaa atatagcgta caattacact gatgaatcta ataataaata
    13081 ttttggagct atatctaaaa caagtcaaaa aagcataata cattataaaa aagacagtaa
    13141 aaatatatta gaattttata atgacagtac attagaattt aacagtaaag attttattgc
    13201 tgaagatata aatgtattta tgaaagatga tattaatact ataacaggaa aatactatgt
    13261 tgataataat actgataaaa gtatagattt ctctatttct ttagttagta aaaatcaagt
    13321 aaaagtaaat ggattatatt taaatgaatc cgtatactca tcttaccttg attttgtgaa
    13381 aaattcagat ggacaccata atacttctaa ttttatgaat ttatttttgg acaatataag
    13441 tttctggaaa ttgtttgggt ttgaaaatat aaattttgta atcgataaat actttaccct
    13501 tgttggtaaa actaatcttg gatatgtaga atttatttgt gacaataata aaaatataga
    13561 tatatatttt ggtgaatgga aaacatcgtc atctaaaagc actatattta gcggaaatgg
    13621 tagaaatgtt gtagtagagc ctatatataa tcctgatacg ggtgaagata tatctacttc
    13681 actagatttt tcctatgaac ctctctatgg aatagataga tatatcaata aagtattgat
    13741 agcacctgat ttatatacaa gtttaataaa tattaatacc aattattatt caaatgagta
    13801 ctaccctgag attatagttc ttaacccaaa tacattccac aaaaaagtaa atataaattt
    13861 agatagttct tcttttgagt ataaatggtc tacagaagga agtgacttta ttttagttag
    13921 atacttagaa gaaagtaata aaaaaatatt acaaaaaata agaatcaaag gtatcttatc
    13981 taatactcaa tcatttaata aaatgagtat agattttaaa gatattaaaa aactatcatt
    14041 aggatatata atgagtaatt ttaaatcatt taattctgaa aatgaattag atagagatca
    14101 tttaggattt aaaataatag ataataaaac ttattactat gatgaagata gtaaattagt
    14161 taaaggatta atcaatataa ataattcatt attctatttt gatcctatag aatttaactt
    14221 agtaactgga tggcaaacta tcaatggtaa aaaatattat tttgatataa atactggagc
    14281 agctttaact agttataaaa ttattaatgg taaacacttt tattttaata atgatggtgt
    14341 gatgcagttg ggagtattta aaggacctga tggatttgaa tattttgcac ctgccaatac
    14401 tcaaaataat aacatagaag gtcaggctat agtttatcaa agtaaattct taactttgaa
    14461 tggcaaaaaa tattattttg ataatgactc aaaagcagtc actggatgga gaattattaa
    14521 caatgagaaa tattacttta atcctaataa tgctattgct gcagtcggat tgcaagtaat
    14581 tgacaataat aagtattatt tcaatcctga cactgctatc atctcaaaag gttggcagac
    14641 tgttaatggt agtagatact actttgatac tgataccgct attgccttta atggttataa
    14701 aactattgat ggtaaacact tttattttga tagtgattgt gtagtgaaaa taggtgtgtt
    14761 tagtacctct aatggatttg aatattttgc acctgctaat acttataata ataacataga
    14821 aggtcaggct atagtttatc aaagtaaatt cttaactttg aatggtaaaa aatattactt
    14881 tgataataac tcaaaagcag ttaccggatg gcaaactatt gatagtaaaa aatattactt
    14941 taatactaac actgctgaag cagctactgg atggcaaact attgatggta aaaaatatta
    15001 ctttaatact aacactgctg aagcagctac tggatggcaa actattgatg gtaaaaaata
    15061 ttactttaat actaacactg ctatagcttc aactggttat acaattatta atggtaaaca
    15121 tttttatttt aatactgatg gtattatgca gataggagtg tttaaaggac ctaatggatt
    15181 tgaatatttt gcacctgcta atacggatgc taacaacata gaaggtcaag ctatacttta
    15241 ccaaaatgaa ttcttaactt tgaatggtaa aaaatattac tttggtagtg actcaaaagc
    15301 agttactgga tggagaatta ttaacaataa gaaatattac tttaatccta ataatgctat
    15361 tgctgcaatt catctatgca ctataaataa tgacaagtat tactttagtt atgatggaat
    15421 tcttcaaaat ggatatatta ctattgaaag aaataatttc tattttgatg ctaataatga
    15481 atctaaaatg gtaacaggag tatttaaagg acctaatgga tttgagtatt ttgcacctgc
    15541 taatactcac aataataaca tagaaggtca ggctatagtt taccagaaca aattcttaac
    15601 tttgaatggc aaaaaatatt attttgataa tgactcaaaa gcagttactg gatggcaaac
    15661 cattgatggt aaaaaatatt actttaatct taacactgct gaagcagcta ctggatggca
    15721 aactattgat ggtaaaaaat attactttaa tcttaacact gctgaagcag ctactggatg
    15781 gcaaactatt gatggtaaaa aatattactt taatactaac actttcatag cctcaactgg
    15841 ttatacaagt attaatggta aacattttta ttttaatact gatggtatta tgcagatagg
    15901 agtgtttaaa ggacctaatg gatttgaata ctttgcacct gctaatactc ataataataa
    15961 catagaaggt caagctatac tttaccaaaa taaattctta actttgaatg gtaaaaaata
    16021 ttactttggt agtgactcaa aagcagttac cggattgcga actattgatg gtaaaaaata
    16081 ttactttaat actaacactg ctgttgcagt tactggatgg caaactatta atggtaaaaa
    16141 atactacttt aatactaaca cttctatagc ttcaactggt tatacaatta ttagtggtaa
    16201 acatttttat tttaatactg atggtattat gcagatagga gtgtttaaag gacctgatgg
    16261 atttgaatac tttgcacctg ctaatacaga tgctaacaat atagaaggtc aagctatacg
    16321 ttatcaaaat agattcctat atttacatga caatatatat tattttggta ataattcaaa
    16381 agcggctact ggttgggtaa ctattgatgg taatagatat tacttcgagc ctaatacagc
    16441 tatgggtgcg aatggttata aaactattga taataaaaat ttttacttta gaaatggttt
    16501 acctcagata ggagtgttta aagggtctaa tggatttgaa tactttgcac ctgctaatac
    16561 ggatgctaac aatatagaag gtcaagctat acgttatcaa aatagattcc tacatttact
    16621 tggaaaaata tattactttg gtaataattc aaaagcagtt actggatggc aaactattaa
    16681 tggtaaagta tattacttta tgcctgatac tgctatggct gcagctggtg gacttttcga
    16741 gattgatggt gttatatatt tctttggtgt tgatggagta aaagcccctg ggatatatgg
    16801 ctaaaatata tgtttgataa aaaattattc ctgtgctact aagaaattat ttttatataa
    16861 taaatattga gatttaatta aaagtcatgt gttattgtaa tacatgactt ttagttaaaa
    16921 tttttctatc atttaataat ctattattct tgactatttt ataataaaat tcatatatgg
    16981 aaatattaat actaaataat taatagttga taaaaaatag ataatatgct aaaagcaaaa
    17041 actaatttag agccttgtaa ctgtttattt gcaattataa aaacatcttt aaacatattg
    17101 actataatat aaaatattaa ctataataca aaacaatatt aattaatttt ctctacagct
    17161 atccctggta tagttatatt tccacccata gttgattcat aagttatagt tccagcactt
    17221 atacctctta tagttatgta atcatcctct aatactcttg aaggagttat tgatttttta
    17281 taactacata gtacaacctt atcataattt ccagacacag ctaatcttat ttgcacctca
    17341 tcaccatctt caataacttg aataacctta ccttcaaatt ttaccttttt gtacttataa
    17401 tcatcaggtg ttctagctaa ttggtcataa gtaataccag tatcatatcc tttcttctcc
    17461 tcttcttctt ttttgcgttg ttcttcagcc tttttagctt cttcagcttt tttagcttct
    17521 tcagctttac gttgattttc agcttcaata gctttctttt cgtcgtcttt cattttgaac
    17581 catggttcag catcagacaa tttgctattt aaagtttcta tttctgtttt ttgagaatct
    17641 ataactgatg tttggttaga aatgacctcc tcatggtctt cagaacaagc tggtgaggat
    17701 atattgccaa aaaacacgcc taaaataaat gccagtagag caataccctt tgtgctctta
    17761 aagaatttaa ttattttctt agagcttcct tttccttcat tactaaattc gttaccctca
    17821 ttttttttag aaaacataat acaatctccc ttttattaga tatattttat tttaaaagtt
    17881 aaaaa
//
LOCUS       DAYASV010000079        17798 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYASV010000079 DAYASV010000000
VERSION     DAYASV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.079"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            529..1389
                     /locus_tag="ACGI68_002474"
     CDS             529..1389
                     /locus_tag="ACGI68_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0138907.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKTLNIKASDYLSLTLLDHLEF
                     LLKRCKKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSE
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1379..3250
                     /locus_tag="ACGI68_002475"
     CDS             1379..3250
                     /locus_tag="ACGI68_002475"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0138908.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQSTTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3270..4724
                     /locus_tag="ACGI68_002476"
     CDS             3270..4724
                     /locus_tag="ACGI68_002476"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0138909.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYEMPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLGKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            5749..6957
                     /locus_tag="ACGI68_002477"
     CDS             5749..6957
                     /locus_tag="ACGI68_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138910.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            6988..8367
                     /locus_tag="ACGI68_002478"
     CDS             6988..8367
                     /locus_tag="ACGI68_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138911.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            8473..9663
                     /locus_tag="ACGI68_002479"
     CDS             8473..9663
                     /locus_tag="ACGI68_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138912.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSENGGKTLLLRADMDALPIREETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGNVPNAIPNKASMSGTLRTFDQFVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEVLLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(9791..11119)
                     /locus_tag="ACGI68_002480"
     CDS             complement(9791..11119)
                     /locus_tag="ACGI68_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138913.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(11737..12951)
                     /locus_tag="ACGI68_002481"
     CDS             complement(11737..12951)
                     /locus_tag="ACGI68_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFH0138914.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYKI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            13377..14093
                     /locus_tag="ACGI68_002482"
     CDS             13377..14093
                     /locus_tag="ACGI68_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0138915.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDEAATKFGLE"
     gene            14228..14854
                     /locus_tag="ACGI68_002483"
     CDS             14228..14854
                     /locus_tag="ACGI68_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0138916.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(15054..16598)
                     /locus_tag="ACGI68_002484"
     CDS             complement(15054..16598)
                     /locus_tag="ACGI68_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0138917.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(16624..17265)
                     /locus_tag="ACGI68_002485"
     CDS             complement(16624..17265)
                     /locus_tag="ACGI68_002485"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HFH0138918.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            17535..17729
                     /locus_tag="ACGI68_002486"
     CDS             17535..17729
                     /locus_tag="ACGI68_002486"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFH0138919.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFD"
BASE COUNT         6581 a         2090 c         2933 g         6194 t
ORIGIN      
        1 aactaaattt aagtatttat atattagtat atcttactag catttaaatg aaactaaatt
       61 tgaagcttta cctattaata tatcttaata gtgattaaat aaaactaaac ttaagacttt
      121 atatgttaat atatcttaag aatttatttg ttaaaataca gttcattata tttaaaagca
      181 ttactatgat aattcataat agtaaaatag ataatggttc agacaatatt ataaatacta
      241 gtgacggtaa aatagtatca atttagtata taaaccttgt cagataatcg cataacagag
      301 cttgtagtta tagaataaaa aaatatctag gaaaccgttg acagatttat atggatagta
      361 taacatatag aatatgaaat taaacaaaaa agaattttaa ttatggcgac gtagtcataa
      421 ttttgagtat gttactgatt agcaggcaga actcaagtat atatgtgaaa aatagaggga
      481 tactctatac atatatgctt gagttctttt tattgaaggg aggaatatat gaaaataatt
      541 cagattttaa ataataatgt agccttagta aaaagaggta aaaatgaaat tattgtattt
      601 gttaaaggaa ttggctttaa aaaaagagtg ggacaagtaa taaatgaaaa tgaaatagag
      661 agaacatata tactagattc atacgatatg ctagaacact ttagttatct actacaaaac
      721 tcagactcca ataatatagt tcttattaat gaaataattg aatatggtga gaaaacttta
      781 aatataaagg caagtgatta tctaagctta accttactag accatttaga gtttttactt
      841 aaaagatgta aaaagaatca atttataaaa agtcctttgg tatggaatgt taaaagattt
      901 tatccaaagc attttgaaat tggaatgcat gcactaaaat taattggtga tagtaaggga
      961 attaatcttc ctgatgatga ggcagtatca atagcacttc attttgtaaa tatggaggta
     1021 aataaagagt ctcatgatag tacaattgta gagttgagaa ctttagcaga tatagtttct
     1081 ataataaaat atcattttaa tgtagagcta gatgaaactt caacaaatta tatgagattt
     1141 actactcatc tacagtactt tatccaaaga attatgtcag aggaagcttc aatagatgaa
     1201 gataattcta tagatttata taaacaagta agtcaacttt atccaaagtc atttggaagt
     1261 gttcaaaaga taagaaaata tataaaatca caatttaatg tcgatatatc tataagtgaa
     1321 gaaacttatt taatgttgca tataaatagg gtgactcaaa gactggaggc aaaatcagat
     1381 gaaatataaa gatttaaatg aaaatataat aaaacttata gggggaaaag aaaatataaa
     1441 gtcggtagcg cattgtgtta ctcgtttaag atttacttta aatgatagga gtatcgctca
     1501 aacagaagaa attaaaaaat taaaaggagt aattgatgta gtttctaatg atgttgctta
     1561 tcaaattata ataggtactc atgttgtaga tgtatataac gagtttatgt ctatgatagg
     1621 tttaagttca agtgaatcaa gtataaaaag tgaaagaaaa aagggaatta gaggtatcaa
     1681 aggtatattt aattccatct ttgtggtagt ctcagagact atgacttcag taattgaagt
     1741 actacttgca gctggtatac ttgcaagtat attagcactt ttaaatatgt ctggcatact
     1801 tcagagtgac agtcctacat atcaaatatt agatacatta agaagtgcta catttcactt
     1861 cttaccagta ttaattgcaa tagcatcagc aaaaagactt aatgtaaatc catatatagc
     1921 agtagtatta gcagttacgt tattatcttc atcaatagat ggtgtagaag gattaagttt
     1981 atttggtata aatttacagt ctacaactta tgcaaacagt tttattccaa tacttttagg
     2041 agtatgggca atgggaatta taattgaagg tttgaaaaaa atattaccta aagcactaca
     2101 ctatttttta gttccagtat tatctttaat aataactcta tcagttacat taactgtatt
     2161 tggacctatt ggtatgtgga ttggtgatgg attaaactat gcatgtatgt ttttaattga
     2221 tacattgggt aattggagtg ttgttgcact gtatgcagct ttacaaccac tcttaatagt
     2281 ctttggtgca gcaaacttta ctatgcctat atctttaaat tctgtgacaa cattggggta
     2341 tgacccaatt tttatgggcg ctgcaacaat atctgatatt gcagtttgtg gtgcaatgtt
     2401 tggatatttc ttaaaaacta ggaataaaga acaaaaacaa ttatttggta cagtaagttt
     2461 tagtgcactt atgggagtta cagaaccttc agtgtttggt gtgtttatga aatatagaag
     2521 accatttgta gcagtaatga taggtggagg tataggtggt ttaatagcag gtttagctca
     2581 agttaaaaca tatggaatgg tttggggatt agctgcatta ccaacgtatc ttgttggagg
     2641 agaggtatct aactttacat ttatgataat aagtgtagta gtatcatttg tagcagcaac
     2701 agcagcttcc tatttactag gaataccttc agatgaaaaa gaagaagtta agggtgataa
     2761 tgaaaaagaa ttagaactaa taatcaacga gaacaattca agaactatta gtattggaaa
     2821 tgttgcaaaa ggtcaggtaa ttgcacttgg ggaagttaat gacaaagcat tttcctctgg
     2881 agcattaggt aaaggggttg gagttatacc tagtgaatca aaatcaactg ttatatctcc
     2941 tgtagatgga actataacta tagtatttcc aactaaacat gcatatggta tagtgactga
     3001 tgaaggatta gaaatattaa ttcatattgg gatagatact gtaaacttag atggaaaatt
     3061 ctttgaaagt cttgtaacac aaaatcaaag ggttaaaaaa ggagatgctt tagcagtatt
     3121 tgaatctgat aaaattatag aggaaggctt tgattctact ataataactg tagttacaaa
     3181 tactagcgat tatttagatg taatctcatc aaatgatgaa tcagaagaat tattaactgt
     3241 aatagtataa attttaggag ggaaaattca tgaatattaa agatacattt ttctggggag
     3301 gaagtattgc agcccatcaa tgtgaaggtt catgggatag tgacaacaaa ggacctgcaa
     3361 ttatggattt tgtaactaag ggctcttatg aaatgccaag agtaattact gataaaatag
     3421 aagaaaaatt ggattatcca tctcataatg gaatagattt ttataataga tataaagaag
     3481 atatagcttt atttaaagaa atgggattta gtgcattaag aatatctata gattggtcaa
     3541 gaatttttcc aaatggagat gatgaaaatc ctaatgaatt agggattaaa tactatgaag
     3601 gtctgataga tacattaata gaaaataata tagaacctat tgtaacacta tatcattttg
     3661 aattacctat gaatttagta cataaatatg gttcatggaa taacagaaaa cttatagatt
     3721 tgtatttaaa gtacagtgag actgtaataa gaagatttga tgataaggta aagtactggg
     3781 ttacatttaa tgagatgaac catattgacc ctcaaacaga gcactcggat atttttactt
     3841 atatgatagc aggtattaag tatagtgaaa tagaaaataa ggttcaaaca ttggcaaatg
     3901 tagggtataa tatgactttg gcgggtgtaa aggttgcaaa gttggctaga gaaatcaatc
     3961 ccaacaataa agttggatgt gtatttggac taaatccaat ttatccatac aattgtaatc
     4021 ctagtaatgt gttaaatgca tttttagata atgaccgtga ttattatcaa attgatgcaa
     4081 tgtgcaatgg gaagtttcca caatataaat taaaagaata tgaaaaaaat aatataacta
     4141 ttgaagtgac tgatgaagac aaaaaagcat ttaaagaagg taagttagat tttataggac
     4201 ttaattatta tttttcaagt gtatctactc caaaagaaaa tttatcaggt gataaatctt
     4261 tatttggtgg gattcaaaat ccatatttgg aacaaagtaa gtgggggtgg gcaatagacc
     4321 ctataggttt aagatttacc ttgaactata tttacagaaa gtatcaatta cctatcttaa
     4381 ttacagaaaa tggtttaggg gcatatgata aaatcgaaga agatggaact ataaatgatg
     4441 attatagaat tgaatattta ggtaagcatc tatctcaaat gaagtctgca attgaagaag
     4501 ataatgttga atgttttgga tatttgatgt ggggaccaat tgatttagtc agtgcaacta
     4561 caggtcagat gagtaagaga tatggattta tctatgttga cttagatgat gaaggcaaag
     4621 gaagttttaa gagatataag aaaaaatcat ttgattggta caaagaagta ataaaatcta
     4681 atggagaagt tttagatttt tctacggata tagtagtagg gtaggaataa gataaataga
     4741 agttaggaac taatgttaat tagtgtctag taaagcattt aaaatagagt atagggtctt
     4801 taaagtaaag tgtgtattct tcttaaaagt aatggcaact aaaattacta taaaaaggag
     4861 aatacacatt ttttatgttt atacttaagt aaatatttag acatattcat agtataattt
     4921 actcattata taaaaatagt atgatttact cactatataa ctttataaat tttctggaaa
     4981 aatgtcagaa tatattgaca aagaaaagat ttaaaattat aatctatatt aagaatagtt
     5041 tgacaatata aaaacaagta tttttataaa tagtgaaatt atttttattg tcaatttaag
     5101 aataatttag gactactata aagataaagg tatagatgtc attatatgat aaattatact
     5161 tgtatagaaa gaattttaaa gtatggctta tcattgattt aatataataa ataatttttg
     5221 tttctagtaa tagttcctag gaataagata tctaaattcg tttagaaata gtctaattta
     5281 caaaggaggg gatgcactaa tagataaaat ttctatataa agaagactta aagtgaagta
     5341 tattctgata atattggttt aatgggtata ttattatttc gttttagata ttttaagaat
     5401 aatttaagac gtttataaaa atgtattcta tatctaatat ttaaataaat ataagtcttc
     5461 caacatgtat actaatatag agagtagttg aagtacaatt ctaactcgta aaattatgta
     5521 gaaagtaaaa atcaaaaaga aatctagtaa gttgataact atagttaata tttagagagt
     5581 taactctagt tatatttatt tagatatttt aggaataatt taggacgtat ataatatcat
     5641 caaaaaatat caagctatac catatgtagt taagaataac tgcataatat acattttata
     5701 attattgttt tattgataaa aaattattat atttaggagg aaacaacaat ggaaaatttt
     5761 tggaatccac ttacttgctt tactgtatgt gtaatgattt atgcttttag tgagttttta
     5821 tctaagaaaa caaaaggtgc aatttcaaca ttgttatttg tatgtattat atttttacta
     5881 ggattttgga caaatatttt accaaaagac attacaggag catcagggat attagctgca
     5941 atgagcaact ttggtatagc tttattaata actaacttag gaacacttat aaatcttgaa
     6001 gacttatgtc atgaatggaa aacggttgta attgcattag taggtattgt aggaatagga
     6061 gttggctgtt taacagtggg tagtatgtta tttggcagag agtatgcttt aatagcagca
     6121 cctccaatag caggttctac tgttgctggg attatcgtaa caactgttgc agaagctgca
     6181 aatagacctg aacttgctgc atttgtaatt ctagtattat catttcaaaa gttctttggt
     6241 attccgataa ctacatattg tataaataaa gaattaaaat ttaaaagaga taacggggat
     6301 tttttgaaag atgagtcaaa aagtaatttt aaacttccaa gtatgagaat ttttaaagaa
     6361 gatttaggta aaaaatcatc tactctagtg tatcttgcaa agctttcatt tgttgctatg
     6421 attggtaatt ttgtaggatt ggctacttta ataccaggat ttacccctgc aaactattat
     6481 ttaaatccta atatagcatg tttgttagta gggttgattt ttacacgaat aggtttttta
     6541 gaaaaaggaa ttttagataa atctcaatct aatggaatta taatgtttgg ttgtatgttg
     6601 atgttacctg gaggacttgc taaggtaact ccaacagcat tgtttgggat gattgttcca
     6661 actataggaa tacttgttgt aggttctgta tttataatat tatttactgc aattgtagga
     6721 aaactattgg gatattctcc aagaatgtca gctgcaattg gtgtaacttg tatgctagct
     6781 tatcctgcaa cacagataat ttccaacgaa ggtattgatg ctatggaagg aactgaagaa
     6841 gaaaaacaaa gggcaacaga ttatttatta ccaaagatga ttataggagg atttgttact
     6901 gtaaccattg catcagtggc atttgctagt ataataggtc caattatatt tggttgatag
     6961 taaatatact tataggagga ataggaaatg ataggaagta tatctataaa tgagatagat
     7021 aaaaaaagat tcaaacttga tgatttaagt aagaaaatat gggaaaatcc agaaaaagca
     7081 tttaaagaat ttaaagcatg tgaaaataca gcaaactttc taagaagtga aggatttgat
     7141 gtggaagtag gtgtaggagg tttagccacg gctattagag ctagttttgg ttcaggtaaa
     7201 ccagttatag gttttatggg agaatttgat gcattacctg gtttaaatca aaaagtttca
     7261 acaaagcaag aagcatttga attgggtgca tatggtcatg gatgtgggca taatttatta
     7321 tgtacagcac atgtaggggc agtagtaggg cttaaaagag agatgataga aaataactta
     7381 agtgggacta tagtatttta tgcatgtcca ggagaagaac aattaacagg taaaggattt
     7441 atggctagag gtggagcgtt tgaagggtta gacttagcta taaattttca tccaaataaa
     7501 attagtgaag ctacaattgg tacttcaaca gctgttaact ctgtaaagtt tcactttaag
     7561 ggaaagactg cacatgcagg gtcagaccct caaaatggaa gaagtgcact agatgctgtg
     7621 gaacttacta atgtgggagc taattatttg agagaacatg ttacttctga tgtaaggatt
     7681 cattatacaa ttacagatgg aggagtagca cccaacatag ttcctgataa agcatcagta
     7741 tggtattata caagagcttt aagtagagaa gctgtagaaa gtacatatga aagacttgta
     7801 aaggtagcaa aaggagctgc aatgatgact gaaactgaag ttgaagtaga gtttttagga
     7861 gggtgttata atactttaaa caatcatgtt ttagcaaatc ttgtatcaga atgtatgaat
     7921 gagattaaac aagagccttg gactcgagag gaattggatt ttgcagctga attagataga
     7981 caaagcccac aacaatataa ggctatgata agtaaatata atttgccaga aggaactcat
     8041 ctatattatg gtggaggaaa tgttacaaac tttaatagtt atggctctac tgatattgga
     8101 gatgtaatgc atatagttcc gacagcatac ttttttacag ggtgtacgaa tctaggagca
     8161 ccaggtcata gctggcagtt tacatcttgt gcaggaagtt ctataggaga aaaaggtatg
     8221 atatatgcat caaagattat ggctatgttt ggagctaaaa tattaaataa tccagagata
     8281 gctaaaaagg caaaagaaga atttgataaa tctatgaatg gtaaaacata taaatgtcct
     8341 attccagatg atattccaac tccttaaaaa gacatgaaat ttaatatata aaattataat
     8401 ttttatatca gcatcatttc attactaaaa gaaagtgaga ttttgctgac tatctgatta
     8461 ggaggaagga atatgaaaaa tttttttaaa gaagcgcagt caatagaagg ggaaattatt
     8521 agatggagaa ggtatttaca tgaaaatccc gaaattggtt gcaatctacc gaaaacagta
     8581 acctttgtta ccagtacttt agaaaaaatg ggatattctc cgaaattgac gtctaataat
     8641 ggaatagtag ctgttttaga cagtgaaaat ggtggaaaga ccttattatt aagggcagat
     8701 atggatgcac ttcctattag ggaggagacg gatttacctt ttaaaagtaa gaataaagat
     8761 gcatctcata gttgtggaca cgataatcac acagcaatgc tcttaggagc agctaaaata
     8821 cttatggata ataagcactt acttaaaggt agagtaaagt ttgtatttga accagatgaa
     8881 gagactacca gaggtgcaaa atcaatgatt gatgatggta tattagaaaa tcctcaggtc
     8941 gatgcagcta tggcatttca cagtatggtg ggtaaatttc ttaacacagg tcaagtagct
     9001 tattcacgtg gtccagctat ggcatctgcg gatatatttg aaataacagt agaaggaaaa
     9061 gcaactcatg gagcaagccc tgaatttgga gtggacccaa taaacataat gtcgcatata
     9121 caaatagcat taaacactat actttcaaga gaaaaacctc agaaagagcc tgtagtttta
     9181 actattggta tgtttaatgc aggaaacgta ccaaatgcta taccaaataa agcatctatg
     9241 tcaggaactt taagaacatt tgaccaattt gttagagaaa aattaaaaaa tagaattatt
     9301 gaaatatcta caggaatagc taaatcattt ggaggaaatg ctaaagtgga gtttaaaatg
     9361 gggactcctg cagttataaa tgatttggat gtaggagatg agattgtatc ttatgttaaa
     9421 gaagttatag gaaatgaaaa tgtaatagac tttccatctg caatgggttc agaagatttt
     9481 tcagaggttt tactaagggt tccaggagta tttttatgga ttggaatggg aagtattagt
     9541 cagggatata attttggtat gcataatcct aaagcaattt ttaatgaaga gggattagtt
     9601 tatggttcag ctttatttgc tcattgtgca gtaaaatggt tagaaaataa ttttagtaga
     9661 taataacata aaatattatt aaatatcata aaaggtatat cttaaaaaga tgcaaagata
     9721 taccttttag aaagtcatat ataattttac ttaatattag atgataaaat atttttatgc
     9781 attttggatt ttaatattta ttttctgata taaataattt gtataccctt tcaggtctgc
     9841 cttttgttga gttttgtttt tcatataata tagtagcgtt tccagacttt aagagactac
     9901 ttagaaatct attagcagtt ctaactgtta catttaaaca tgaagataat tccttaggag
     9961 taacttcatc tctttgtaga atttcagtaa cagatataat cttttgaata gtaagagtag
    10021 acaatttaga acttttggca attgtattta tatgtggagt tactttattt gaaacagtaa
    10081 gtaacttatc acacagtaaa ggtcctattg tctcataatt ttcgttaact aagaaactgt
    10141 aatttaaagg atttatttta gattccttat tagcacttat ggcactagac atagcttgct
    10201 ttatatcatt accaattcca tatccaatac atactttaat atttgtacta ttttcaatag
    10261 attcttttat ataacatgca gtaaaatttt tagttaatga tttaatcgtt tcacatttag
    10321 tgaaaatttt aaaagaatta ctattttttt caattataaa atcggttaat ttatcttttt
    10381 taatactatt taatatttta taaacttggt ctagagtaga gtcatcataa tttgtatctt
    10441 taactgttat atatattaca cttggttgat ttcccttcat tctatgtaaa tgaatatctt
    10501 ttattaagtt attaaaagta ttttttaata aatctatatc agggtatatg aaatgaaagt
    10561 ttactcctgc attttgtaac ataggaacaa tactactaaa acgagtagta gaaaaatcga
    10621 ttttcccttc attccataga ttaatatgtt tttttgcaat ctttttttca agttttgcta
    10681 tgtcagataa actcatttta gatgcatttt catcaatact atccattaag tctccaaatg
    10741 tgccatttga taaataatat gatatatctt ttggattatc tgcccagtct gttaagtcaa
    10801 agtatgctct ggaaaaatct aatgtattat atttaaagat aagctttaga aacatatcat
    10861 agtaactttg taagtcagta ccaaaatcaa ctagaggttt tttaatagtt ccagcctttt
    10921 ttgtaatagc ttttttgggg atggggccac taactgcaaa tccatcaaac ctatcttcta
    10981 tttctaaata taaatctcca acatgagaaa aatctctata gatatatatt tcaatttctg
    11041 cattaatatt taactttttt atagaattat ctacaaactc ttttaaaaat tcggttgtta
    11101 tgactgctat tttcatcata gattacttcc ttttcaatta ttataattat tttatattaa
    11161 atattacatt aatctattgt tctagttaaa atcgtatttc aattaaattc aattaaaata
    11221 aataactctt tataaaaagc aattatttag gtagaggaat atttgctaac tttaatagtc
    11281 tttgttaact ttaattttat ttaagttatt atagtcttac taattttata attgtagatt
    11341 taactacttt atgttctttt attgtttatg tagttgattt agaagttatt ttaaccattt
    11401 attttaattg aagttgtttg acttttaaat cctttgtgta atctttgttt ttctagccat
    11461 gataaaagta tttctatttg tatcactata tataataata tcatgacaaa atatttattg
    11521 tctaactaat tataatctat ccaaaatgaa aatgggtgtg agatattatt acaacaaatt
    11581 gtattagtat taaaaagatt aaaaaattaa aaaactaaac agttaaataa ttaaacgatt
    11641 aagtgactgt catataacaa tttataattt tatttaacag ccacttaggt atttaagagc
    11701 gatagaattg agtgtatatc aaattataat aatttcctag taaataattg aaaaataagg
    11761 tagtccattt ttatatttga tatctggttt accttgaata agtggatata gataatttag
    11821 ggcttcatca gttatataat tacattcttc atttatccac tctttaggaa aatattttgt
    11881 cttgttagct attttagaaa tatcagtatg actaacttca tacttataag gattattgca
    11941 tgttctatta atgatagcca taactcctgt atatcctttt ttagatagtt ctatcccata
    12001 tgcaccaact tcagctgctt gttttaaatc agtatctgaa gctaaatgca tagcacatct
    12061 ttgtattaca ccaagttcta aggtttttac tcttgttgta attgatgatg atactataag
    12121 attctttaga tagttgctta ctccacctaa ttgggaatga ccaaatttat catgagcaga
    12181 agttttttgt tcgcatataa agttgccgta tttatccctt ataccttctg atgcaactat
    12241 ataagcctta tttttttctt taaatatttt tctaacatct tctataaact tatcttcata
    12301 gaaatcaatt tctggaaggt atattaaatc aacaagtgga gtatcattaa gtctagctag
    12361 tgaagaagaa gctgctaacc atccagtatc tctacccata gtttctacta taaatatacc
    12421 attatttttg tatatagaaa aatctaagta tgtttccatt attagtgtag aaataagctt
    12481 tgcagcagaa ccaaatccag gacagttgtc agttcctaat aaatcattat ctattgtttt
    12541 aggaatacct ataaatttta ttttacaagt agtgtttttg cagtattgag aaaatttcat
    12601 cactgtgtcc atagaatcat ttccgcctat ataaaataat gtatctattt tatattcatc
    12661 tagtatattt ataactctat cataatcata agaatttatt ttataatcag ccagtttgta
    12721 tctacaagaa ccaagaccag acgaaggtgt ttgctttaaa tttaatacat tatctttgtt
    12781 taaggagtct gaatctatta tatttttgtt taatatacct tcaatcccat tgataccaaa
    12841 atatacttta tcaaagtatt tagtttctaa atttttttca taaataccaa ctgcagaagc
    12901 attgataaca gctgtagggc ctccagattg ggcaataata cagtttctca ttttacatct
    12961 ccctttcatt aatattgtta tgaatggtac tttttatcga gtaaatttat atttatagtc
    13021 tgattatata ttaaatgaac tagctatatc aattgagtta gtatagacaa taaatgccat
    13081 aagaatgtgg taaaaaacta actttttata tacaattaat gtctataatt taccacaata
    13141 taaaggtact tttaaggagt agtcaaagtg ttgtgaaaaa aaatatatca gtgtaatatt
    13201 aatttacaaa gcaaaataag ttcacagtta acaaaagcag aataagttca taaaagaaat
    13261 atatacagca ataaaagtaa tgctaaataa aagtaatact ggattaaaaa atattaaaaa
    13321 aatattaaaa actaatattt aatactaatt aaagtaaaat atggaggtat acaaatatga
    13381 aacacataaa aatagcagca ggattagcac atgtaaatta cggtcatata tcagccgtag
    13441 ttaaagaagc aatggatgca ggtgtagact acgtacattc agatgcagca gatatgcatg
    13501 acttaaaaaa tatgcaactt atgggtggtc atcaaataat agaagcaata agaccggtga
    13561 cagataaacc aatagaatgc catatataca caagagattg tgatagatta tttatagaaa
    13621 aaatagcagc agcaggatgt aacttgttga taataccagc agaacatttt ttaggtgctc
    13681 ctcttgcata cataataaat tactgtagag aatttggtat gaaagtagga ttaacaattg
    13741 gatgttacac accactatgt tttgttgatg aagctatata tgatatagat agattgcaaa
    13801 tagtcgttca tggtgttgat gaaactgatg gaaaagataa ctggggatgg agaaagagtg
    13861 ctgttgattt agtaaaaaga gctagaaaaa tgatagacga aaagaatcct aagtgtgaat
    13921 tagcaataga tggtggacta agagcagaca atatggagcc attaatagaa tgtaatccag
    13981 acgttgtaat attatcttca gcaatattca aggataaaga tggtataaca gcaggtgtta
    14041 agaattgtag aaaagctata gatgaagcag ctacaaagtt tggtttagaa taagataata
    14101 aactatgata aaagagatgc tttcttaatg ttttaaggaa gcattaattt tatgaaaaac
    14161 tacaggaagc tagactaatt taattgattt tttatactga aacaatataa tcataggagg
    14221 ttattaaatg aaaaaagaaa tgataaaaga ttatttactg ataacaatat cagcattttt
    14281 aatggcatta gcagttaatt ttttctttgc agaacataca ttagctccag gagggataac
    14341 aggattatca gtcgtactta gcacattctt atcaatgcct gttgagaata tatcattagg
    14401 aatatctatg ccacttttag taatgggatt attattttta gggaaaggtt ttggaataaa
    14461 aacattatat ataactctta tgggcccatt attcttaaaa gtaataccac aaactcacat
    14521 aactgacaat ctattagtag caggagtgat aggtgggtta ttggtaggaa ctgctatagg
    14581 gatagcaata ataaggggct gtagtactgg tggaactgat ttagttgcaa tgcttattaa
    14641 taaggtaatt aagtttctga agttgccagt tatattattt atgctagatg gatttataat
    14701 agtagcgtct ggaataataa gtaagaattt tatgatatct atatattcat tgatatcttt
    14761 attactcata ataaaaacaa taggatttgt aactacaaag tttggtaaga atactgaaga
    14821 aattaataat ataaataatg cttgtgctca ataaaaataa taaaatttac tttaaatata
    14881 aaagtttaaa aaatataaaa gcttaaataa aaagattaaa tataaaaaat gaaggtgtct
    14941 caaaatgaat tttttagttc atgaggcact ttttctatga aatcaaatat atttttatca
    15001 tttggacaat gaaaaatagg tttatctcta tgttgagaca agcctatttt tgtttattta
    15061 taagataata ttttattttt ttcagaatat agttttttta taattgtact aagtctagaa
    15121 atatcattct tacttaaaag agcatttata tttatcagtt cagcattatg ttcaaagtct
    15181 acaggagcag tggttagtat taagtccaca tcatcaaagt tcgtaaatga aatagaagat
    15241 agaggtatag atttaactat attaatctga ttaaattcaa aacttaactt ttggactaat
    15301 aaattagata caccatcact acagtgacat attaaaatag tatttagggg agatttgctt
    15361 ctatctattg ctgatattaa atataaagca agatatccta tttcgtcatc attgacttca
    15421 taattcatca actgtttgat aacagtatta gactctttag cagccttata agtgtaagga
    15481 aatgttctct ttatatcatt taatatggga tttgtaatag taaacccata gtttattctg
    15541 tgtagaactg atttaaggtg attagcaaga gactttatta atattaaatc atcctttaag
    15601 tttaaattta gtatacttcc ccaatgaaca gctattgctt cagatagatt atactctgta
    15661 atttcttcat ttttaagagg ttcaagagta gtagttttct ttattgattt aagtaatcct
    15721 tctatatgaa gaaggatata acagacctct tcatcaataa attctacgtc aaaagcttca
    15781 cttaggttag cacataaaat ttttgatttt tcaaagtatt catgttctaa taattctcta
    15841 aaaaaacctt cagataagtt aatatatctt ttaattgaaa ttcttttaat acatatagct
    15901 atatgtatta agaagtttat ataatactca tcactaaggt tacttttttc tacttgtaag
    15961 gtgtgtagag tgatttgaga taatttctca aaatcatagt taaatagctc cttaaatatt
    16021 tttgttgatg gggtatcttt gcattcatta gatttcgaga ataatatttc tttaagttca
    16081 gtataacttt tattaaccgt agcaagttca aaaattgctt ttcgaatatc tttctctttt
    16141 ccctctaaat atactccatt attggttttt ctaactagag tcactttaaa gttttgcaaa
    16201 aaattttgaa taactaccaa gtctttatgc acagtagctc tactaacatg tagttcattg
    16261 caaatatcac gaatcctaaa cttagaattt ttaataaata atttacttag aatatacctt
    16321 tttctatctt caggagagta tgcatatata caatttgatt tatcattgat atctctaata
    16381 actttaagtt ttatatcttc attacctaga ataataaccc ctgaaccagt ttttttatct
    16441 agggataaat taaactctaa aagccattca tctaaaataa caaggtcatt acgaatagtt
    16501 ttattactaa cttttaattc ttcggcaata gtatctacag taatatagtc tgagctgttg
    16561 agtaataatt cagcaatatc taataatctt ttgttcaatg caaaccctcc taaatcttag
    16621 ttactattta ttaaccttgg accaatctct ttcaaaagta ttggcaccat ctattgtaag
    16681 ctggttaaaa gaagcttttt taaataaatc atatgttaca gctgcataat cacatccaca
    16741 aagagatgct tgagttaagt ggaatacatt tttaatagaa gctgcaacaa ctttagtatt
    16801 atatccattt attgatataa ggttacatat ttctgtaagg ttttttatac catcattccc
    16861 attttcatca tttctaccta taaaagggaa taggtaatca gcacctgcat ttatagctaa
    16921 tgatgcttga tttacattga aaatcaaggt acatgcagtt tttacacctt tactatttaa
    16981 tatgtttata agttctaagc caacttcaga gaaatttatt tttattatag tgtttttatt
    17041 tatatttaat atcttatcac atgaattaat taattcgtat aaattatttc caatgacttc
    17101 agcagttaat atttctggat ttaaattagc acatgtctca ataaattcat ggaagtttac
    17161 tttctcttta agatacatag atggattagc ggttattccc ttaaatccat actttagaca
    17221 cttttgaatt tcatcaagat tagcactgtc tatcaaatat ttcataattt aattctcctt
    17281 taagctattt gtcataaaaa agtgtatata ctctattttt attattaagt caattataca
    17341 ataagattga ttataaaatt ataacatggg tacttaataa aatttacata atgatatggt
    17401 aataataaaa cattgattgt ctaataatta ccataagctt tagtaaataa gagttaacag
    17461 tgaaggtatt gccttaaaaa tcctatgata tgtataattt gaatatgaag gcaataattt
    17521 aagggaggaa aaatatgatt aacttttatc aaccatcaag aataaatttt ggacaaggaa
    17581 ctttaagtga attacctaga ataataggaa aatatggaaa gaaatgtttg ttagttacta
    17641 ctccaaatgt agagccatta gacaagttat ataaaagagt tacattgaat ttaatagaaa
    17701 aaggtataga agttgtacac tttgattaaa aaatagctac gcacactcgt gactttagtc
    17761 atgagttaat agctaaaata gtcagcatat agggaaac
//
LOCUS       DAYASV010000080        17737 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYASV010000080 DAYASV010000000
VERSION     DAYASV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.080"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            103..1125
                     /gene="splB"
                     /locus_tag="ACGI68_002487"
     CDS             103..1125
                     /gene="splB"
                     /locus_tag="ACGI68_002487"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006290 - pyrimidine dimer repair [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HFH0138920.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            1307..2218
                     /locus_tag="ACGI68_002488"
     CDS             1307..2218
                     /locus_tag="ACGI68_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0138921.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            2233..2649
                     /locus_tag="ACGI68_002489"
     CDS             2233..2649
                     /locus_tag="ACGI68_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HFH0138922.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(2749..3141)
                     /locus_tag="ACGI68_002490"
     CDS             complement(2749..3141)
                     /locus_tag="ACGI68_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0138923.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            3282..3677
                     /locus_tag="ACGI68_002491"
     CDS             3282..3677
                     /locus_tag="ACGI68_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0138924.1"
                     /translation="MKALEFLNKAGVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            4080..4286
                     /locus_tag="ACGI68_002492"
     CDS             4080..4286
                     /locus_tag="ACGI68_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138925.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(4598..5341)
                     /locus_tag="ACGI68_002493"
     CDS             complement(4598..5341)
                     /locus_tag="ACGI68_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HFH0138926.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
     gene            5507..8074
                     /locus_tag="ACGI68_002494"
     CDS             5507..8074
                     /locus_tag="ACGI68_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0138927.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            8304..8708
                     /locus_tag="ACGI68_002495"
     CDS             8304..8708
                     /locus_tag="ACGI68_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138928.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            8732..9229
                     /locus_tag="ACGI68_002496"
     CDS             8732..9229
                     /locus_tag="ACGI68_002496"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0138929.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            9232..9633
                     /locus_tag="ACGI68_002497"
     CDS             9232..9633
                     /locus_tag="ACGI68_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0138930.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            9651..10139
                     /locus_tag="ACGI68_002498"
     CDS             9651..10139
                     /locus_tag="ACGI68_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0138931.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            10166..10921
                     /locus_tag="ACGI68_002499"
     CDS             10166..10921
                     /locus_tag="ACGI68_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFH0138932.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            10921..11784
                     /locus_tag="ACGI68_002500"
     CDS             10921..11784
                     /locus_tag="ACGI68_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0138933.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            11831..12310
                     /locus_tag="ACGI68_002501"
     CDS             11831..12310
                     /locus_tag="ACGI68_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HFH0138934.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            12375..13682
                     /locus_tag="ACGI68_002502"
     CDS             12375..13682
                     /locus_tag="ACGI68_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0138935.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVRGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            14010..14132
                     /locus_tag="ACGI68_002503"
     CDS             14010..14132
                     /locus_tag="ACGI68_002503"
                     /inference="COORDINATES: protein motif:HMM:NF021468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFH0138936.1"
                     /translation="MLSNKITKECYKVGYMIREKSIDTYPDSGWGFMTWDENGE"
     gene            14642..15736
                     /locus_tag="ACGI68_002504"
     CDS             14642..15736
                     /locus_tag="ACGI68_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138937.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            15741..15941
                     /locus_tag="ACGI68_002505"
     CDS             15741..15941
                     /locus_tag="ACGI68_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFH0138938.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            15938..16858
                     /locus_tag="ACGI68_002506"
     CDS             15938..16858
                     /locus_tag="ACGI68_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0138939.1"
                     /translation="MMNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKT
                     TTMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCG
                     EITGLSKKEIQIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDE
                     PTSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLS
                     EIKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTV
                     ISTLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            16855..17613
                     /locus_tag="ACGI68_002507"
     CDS             16855..17613
                     /locus_tag="ACGI68_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0138940.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTIYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISVVCLLAAVILFNKKPV"
BASE COUNT         6834 a         1863 c         3184 g         5856 t
ORIGIN      
        1 ttttactttc taataaataa aggagtttaa aaaagggaag attaattaag taatatttta
       61 taaaattctt cgatttaaaa actttaagta gaggtgaaat aaatgtttaa tcccaaaaga
      121 ataatattcg aaaaaggaac tttagacact gatgtgggta aaaaaatata caataaattt
      181 agagataata aagatgtaga gataattaat atatcatcaa ataggattaa agaacatatt
      241 ccaggtgaaa atctatatga acaatataga agtgggaagc agactatggt agttgggatt
      301 agaaaaagtc tgaagtttca aacatgtaaa ccttctgcta attatcaact accacttgtc
      361 agtggatgta tgggaagatg tgagtattgt tatttgaaca cacaattagg tgataaacca
      421 tttataaggg tgtttgtaaa tgtagatgaa atacttgaac agacaaaaaa atatattgaa
      481 gatagaaagc cacaggtaac catatttgaa ggggcagcta catcagaccc agttccagta
      541 gagccatata ctaattcttt gaagaaaaca atagagtttt ttggaagaga ggaaaaagga
      601 agatttagat ttgttactaa atacagtgat gtggatagct tattggatgc tgaacataat
      661 aatcatacag aaatacgatt tagtatcaat actcctagaa ttataaatga acatgagcat
      721 tatacagcct ctgcaaataa aagaatagag tcagctgtaa aacttgctag ggctggttat
      781 cagattggtt tcataatagc acctgtgttt ttatatgaag gttggaaaga agagtataaa
      841 aatctgatta taagtataaa agaagcttta ccaaaggatt ttgataaaaa aattatattt
      901 gaagttatat ctcatagata tacaactaaa gcaaaaaata gaatactaga gatttatcca
      961 gatacactat tgccaatgga aaatgaagat agaaagttta aatacggtca atttggatat
     1021 ggtaagtacg tgtatgataa agaacaatta gatgatatga aagagttctt tcttaaggaa
     1081 ataaatggag tatttaaaaa tagtttaata aaatatataa tatagcaaat cttacctaaa
     1141 gatttgctat attttatttt tatatatttt atgtttataa taaagcaatg agatttagca
     1201 ttatcatata agaaaattaa aaagggcact atctatcaag aacatttttt ctagttttga
     1261 tactataaag gataagagta acaaaaatat caggaggagt tatagtatgc atgcatggga
     1321 gtctattcaa attacattgg atttaataga agcaaattta tcagaagaaa tatcaattaa
     1381 tgaattagca aataaagcaa atttatcacc attttactat caaagactat ttaaaagatt
     1441 ggtaaataag actgtaatgg agtatataaa gcttcgtcgt cttgcaagag cttctgaata
     1501 cttagcagaa cataagaatc gtatacttga tgtagcttta aattttggat ttgctagtca
     1561 tgagacattt acacgttcct ttaaaaaagc atatggatta actccagaaa aatatagagc
     1621 aaatccagta aaactcaatc attttattaa gccagaatta attttaaatt atgctatggt
     1681 tgatgaaggt gttccactga ttgcagatga tatagtgatt gaagtcaatc gtaagacatt
     1741 gagtaatcca aggacatttg ttggaataga aatagaagta cctatttgcc agttggtggg
     1801 cggtgaaaca actggaattg caatagtaga ggaattatgg atgaaacttg gaagtcaaag
     1861 acataatata ccaaatcaaa ttccaggtgg aaatgaattt gctgctcttt atatgggaaa
     1921 tgcaaaagaa ggaaattgta tgtatatggc aggtgtagag gtagagtcag gtacatcagt
     1981 agaaggatat agtacttttg agttgccagc taaagaatat ttagtatgtg gatttgaggc
     2041 agaaaacttt aatgagttag ttaattcagc tgtttttaaa gcagataaat ttatggaaag
     2101 atggatgaag aaacataatt taacaactac tgattttgca attgagatgt attaccccac
     2161 tacacctgaa gcagcatatc tggaacattg gattgttccg gttcctattg aacaatagaa
     2221 aggatgatga atatgaattg gcaagatgcg ttttttcaag atactaaacc tacatttgag
     2281 caaattaatg aatttattaa taatccatta tgggacaagt taaataaaac tttggtttct
     2341 acttataatg tagaacctaa gcttgaatac agtaaatgtt caatgcaaag aggttggaat
     2401 gtaaaatata aaaaaagagg taaatcacta tgtactttat atccacaaga aggttatttt
     2461 aaagctttgg ttattgtaag tgaaagcaat agagtagagg ttgatttatt tattaatact
     2521 tgttgtgatt atatcaaaaa aatatataat gaggtaaact tttttaatgg aagtaaatgg
     2581 ctaatgattc aagtagatag tttattggta ttaaacgata tgcttgaact aattaaattt
     2641 agagcttaac atatagaaca ggatgaatat ataagtcatc ctgtttttat attcaatcag
     2701 ataaattaat atatagtcta taatatgaat aatttagtaa atttaaattt actttatctt
     2761 tctacattct ttgcaactaa gtccacatgt gtttttttct tgcatattag tagcacccca
     2821 attagcaaat tgttgtaaaa taggcacaat acttttacct ttacttgtta atgtatattc
     2881 aacttttgga ggaatttcat tatattgttt acgatgaaca ataccagtag actctaaatc
     2941 ttttaatgat tgagctagca tagtatttgt aatgccatcc aattttcttt ttatgctact
     3001 atatctaatt ggattttcaa ctgctaacat acatataata ggtaatttcc acttaccacc
     3061 gacaatgtct aatgcataac ggattggaca ggttaattct tcaacgtttt tagttatatt
     3121 tttttcagta tttatactca ttttaattac tccttagtta tagtagtatc ttttttatac
     3181 tttatcagaa aaaactgcat acttgttttt tctgtatttt atattaaaat tatatcaaag
     3241 gataatcctt aatacaagta tttaaaaggg gtgtaagaat tatgaaagca ttagaatttt
     3301 tgaacaaggc aggagtttat tatttagcaa cagttgatga gaacaatcaa gcacatgtgc
     3361 gtcctttagg atttgttatg gaatacaatg gtaaacttac attttgcaca tctaatcaaa
     3421 aggatatgtt taagcaactt atttttaatc caaaggttga actttgttgt atagaccaaa
     3481 accttaatac tctacgtatt ttaggtgaag cagtatttgt tacatcaaaa gaaacacaag
     3541 ctaaggcact tgaaataatg ccaagtttag gtcagatgta ctctgtagga gatggtaagt
     3601 ttgaaatatt ctctattgat aaggcaaaag ttagttgttg ttcaatgagt ggtaaaaaga
     3661 ttgatgtaga gctttaatta taattgaatc gacatccata gcgaaaatta agaaaacgtt
     3721 attactgaaa tattgataga tttaatttaa atgcaataaa aagattttta actaatgaat
     3781 atgtaaaaat ttttagttat tagatgtgta tttcaataat gatatactat tttgaataat
     3841 tacatactag aaataagata attaaaattt actttataat gggatagatt tcttttaaaa
     3901 aatattttaa ctataattga tttttattta atctgaaaaa aatattatga ctataaaaaa
     3961 ttgctactta ttatttggat atttttatat ataaataata agtagcatga ctttatacat
     4021 taataatgat agaatataaa ttaatagata agatatgatt ttaaaaggag tgatataaga
     4081 tgagtaaata tagttcttta tgggaatatg ttaagaaaaa caatagtcaa tcgtttaaat
     4141 taacttttga agaaataaaa gatattgctg ggatagagat agaccattcg tttttgaaat
     4201 ataaaaagga attaaatgaa tatggatatc aagtaggaaa aatctcttta aaagagaaga
     4261 ctgttatttt cgataaaatt ggttaagaca taaataatta catataataa aataatttgt
     4321 attgatgatt attacaaata aaaataaagt aaaccttata taagatttac tttattttag
     4381 atgattcttt tttttagctt tatactataa ggtatataaa actaagctta ttaaaaatgt
     4441 tataatttct attgctatca tagaccatat caatccatca agttcataaa agtaattaag
     4501 ctttattaaa tagcttcttt attataattt ctttatttca tataatataa atagtattaa
     4561 aatttttatg ggagttaaag taagcatgta ttattactta gatatttgta taatcaacat
     4621 ctagtaattt acctttttga tgaaaatgat taataagttt tttaggataa ttcattatta
     4681 attcatctgg aaaattaatt tcctttaaga tattttcaga atactcatag ttacctatgt
     4741 caaaacaaat gtgagcatct gaagttagta tgattttagc accatatttt ttgcaaagtg
     4801 tagcaatata cttacagtta tccgagctac catttcttga cacgccttta atagaacaat
     4861 tatttatttc tattaagata ttcttttcta tagctaattt aatgattaat tcatagtcga
     4921 ttgggtagtt tgggtttcca aggtgtccta atatttcaat attatcatgc ttatttattg
     4981 catttattaa agctttagta ttattctcta atgtatttgg agaaaacaca gcttcgtgaa
     5041 atgacaagat aagataatct aaccgaggaa ttacaaaagg ctctaggtct atgtttcctt
     5101 taatatctag tatatttgct tcacagcctc ttaaaattat aatgttattt atgattcttg
     5161 ggatattttg aatgttgtga aagtaccatc tgtggggaga accaggcatc ttaggaccat
     5221 gttcagatgt acctagtatc ttaattccat tttctttaca ataattaata ttttctagta
     5281 aagtgctata gccatgacca ctgactatag agtgggtgtg taaatctgat aaaaaattca
     5341 tataaattcc tcctctataa aagatgaggg taacgtaatt tatattataa aacgaatagg
     5401 aaagaaaatc aatagttaca taattagaca aaaatattaa aatattgtag aattaagctt
     5461 aataataaat gtttaacagt attactgctt ataggggagg ttaactatga cacaaaagaa
     5521 gctatcaagg aaagaaaaag ttttaatttg cttgaaaagg ttaacagaag aacacttgaa
     5581 ctttaataat ggcattagat ttggatttga tgcagaatat atagctactg aagtaggtat
     5641 aaatagaagt aatgctagta aggaattaaa tcaattaata aaagaaagta tcattataaa
     5701 gataaagggt aagcctgttc agtacttata taaagaaagt attgagagta ttttaggaaa
     5761 gaaactaaat aaagggatat ttgataatat taactctata aacgaatata gtgaaaatga
     5821 ttctatcaaa gatgagtata ttttagagag tataaaaaaa gagaacaata tttttaattt
     5881 agtaggatgt gatggaagtt taagatccca aatagaacag gctaaagcag cagtaatata
     5941 tccacctaag gggttgagta cattaataat aggacctaca ggagttggta aatctatatt
     6001 tgcagaatat atgtataagt atagtttaac tataaaagaa gatagtgata atgcaccatt
     6061 tatcatattt aactgtgctg attattctga taatcagcaa ttattattag cgcagctttt
     6121 tggatatgta aaaggagcat ttactggagc tgataaagat aagtatggta ttgtacatga
     6181 agctaataat ggagtattgt ttttagatga agttcataga ttatctgcag agggacaaga
     6241 aatgctattt ctgcttatgg ataaagggat atatagaagg ttaggagagg ctaataaggt
     6301 acacgagtgt agagttatga taattgcagc cactacagaa gacccagaaa cagcaatgtt
     6361 ggaaacgttt ttaagaagaa tacctgtaac tataaagatt ccgtctttag aggaaagagg
     6421 ctttcaagag agaatgaagc ttatatgtta tttttttaaa gaagagagtg ttaggattgg
     6481 tgttaagtta aaggtttcaa aagaagttat taaggcattt attttatata agtgtaaggg
     6541 aaacataggc caattaaaaa gcgatataca attaatttgt gcaagagctt ttttagatta
     6601 tatgacctat aaaagggaat gtgtatatgt aaggctctct cttctaccaa ataatattag
     6661 agaatcatta tatggaaata atagaaaaga ggagtttgta caaaatttca gttttattgg
     6721 aaaagatgat attatttttt taccagaaga aaaggatttt gaaagtgaaa atttattaat
     6781 agaaaataaa aaatatgatt tagattttta tggaatgata aaagaaactt ggaataaact
     6841 tcaacaagaa ggtattaaag agtcacaaat tagaagtgtt atagatggag acatacagaa
     6901 gtattcctat aatttaatga atacttttat atataatagt agtaatcaaa cagcttataa
     6961 caacattgtt aatgattcaa ttgcaactac tgtaaaatat attttacaaa agcatgataa
     7021 atggagcaaa agagaaggat tagataaatt aataaaagct atagctttac atattcagaa
     7081 tttaattgaa agaataaaga ttggaaatat agtaaagcat cctaataaag atgaaattat
     7141 gaaggaaaga gcatatgaat ataacatagc aaaagaaata ttatctaaca tgtcattaat
     7201 gtatggagta gagtttccag aagatgaagc aattttttta gcaacatttc tttatttatc
     7261 gtatgtggga ttaaatgagg agagtatagc aatattagtc ataatgcatg gtgaatctac
     7321 agcatctagc atggtgaatg tagccaatac tttactggat tgtaaccatg cagtggcaat
     7381 taatatggga ttagaagata gagttcagga tattttagta caagctgtag aaatagccaa
     7441 taaaattgat aaaggaaagg gaattttaat tttaactgat atggggtcta ttttaacatt
     7501 tgctaaagtt attcgtgatg ctacaggaaa agaagtaaaa gcaatagata tggtaagtac
     7561 acctattgta atagaagcaa ctagaaaggc tctaactcca gaaatgacat tagagaaatt
     7621 atattttaat attatagaat ctataaagaa acattatgga gatatggaag ttacatatgc
     7681 tgaaaaagat aatggtggta gatattttga tagattacta attgataata ttagtaaaac
     7741 tttgactttt ttaaatgggg aaaaggctta ttttatatta aaagaagtaa ttaatagaat
     7801 tagtgaacac tattctttag taattcaaga tgaactttta gtaaagttta tatttcattg
     7861 ttcatgtatg attgaaagag taataataaa agagtcttta gtttataaat catatgaaga
     7921 aagaattagt agaaataaag aattatattt agttataaaa gaaagtttta agctagtaga
     7981 agagacattc gatataatta tttcagatat ggaatatgca aatatattag atatatttga
     8041 atcacaatat gatacagata aattcaaaag ttgatacaaa atttaaaaat agccaaaagg
     8101 ctatcttttt tttacttttt ttgatacaga tttgatacag gttgaaaaaa tcttttgata
     8161 catatcgaaa taataagttt ttaaaaaaaa ttgaataaaa agttttgatt aaatcctgta
     8221 aaatagccat ttgttattga tacacataag ttggcacggc aattgcttat ataaaaagtg
     8281 acaagaaaag tgaggtgaaa ttggtgatag gaataatagt tatgactcat gggagtttta
     8341 gtgaagaaat tataaaaagc tgtgaactta ttgcaggacc agcagaaaga acagcagcaa
     8401 ttaaactaaa taggaatgat aatattgaag acttaaataa aaattttgta gaaaagttaa
     8461 atgaattaga tgaaggtgac ggagttttag tattagctga cctattagga ggttcgccaa
     8521 gtaatgttgc aagtttaaac cttaaaaaag gtggcaagtt tcatgcactt actggagtaa
     8581 atcttccaat gctacttgaa gctttaatta atagagaggg aaaaagttta gaagagttag
     8641 cagaagcatg tattgaagca ggacaaactg gtataaacaa tattaacaaa gttcttagct
     8701 caatgtaata gaaaaaggag tttgattaaa aatgtcttta gtaaaatatt gcagatgtga
     8761 tgatagatta atacatggtc aagttatata taaatgggtt aagcatctag gtgtaaaaaa
     8821 gatagtagta gtagatgatg aaacaaccaa tgatgttatt gctaaaggtt tgataaaaat
     8881 ggcagcacca aagaatattg atttatcaat attaactgta agtgaatcaa ggagatattt
     8941 ttataataat caagctgatg ataacgtttt tgtattaatt aaaaatttag atactgcaaa
     9001 tagaatgata gaagaaggga ttaatatcaa aaaacttatt gttggaagaa tacctacagg
     9061 aataggaaaa aagaaaatat ctcaaaatgt atacattaat aaaaaagagt ttcttttaat
     9121 agatgaattt attaaaaaga atattaatgt atcaattcaa atggtacctg atgaagaaga
     9181 agtagaatta aaccaaaata ttaatcgcta taaaggagag tttatttagc aatgaataaa
     9241 tataaggtaa tcatactgtg ccatggaact tggggaaaag tcttagtaga agaaacaaat
     9301 aaaaactttg gattgtcaaa tcaatatgaa gttttatcac taagttctga aaaagatgtt
     9361 tctgtattta tgaaagaggt tgaagaagta gtagataaag aagatgtgaa aattataata
     9421 tcagatttat atggaggatc tacatcaagt gttgcaatag ctgttgctat aagaagagga
     9481 atagatgcaa taaatggact tagcttacag acaattttaa tcgtagatga agaattaagc
     9541 aaagaagata aattacatac attaccagaa agaatcgtca aaaaaaataa taacctttgt
     9601 gtggatttaa ttaaagaatt taaaaaaata taaattatga ggtgaatata atggcaaaaa
     9661 taacattagt aagagtagac cacagattaa ttcatgggca agttataaca aaatgggtaa
     9721 aaatcgctca agctcaaaaa ataataatag ttgatgactt cttaggtcaa gatgaattta
     9781 tggctgacat atataaaatg gcagcaccat caggagtaga agtagttatt ttaacagcag
     9841 aagatgcggg acaagcattc caaaacaaca ctttaggaga taaaaatata tttattctat
     9901 ttaaaaatgt tgatatggca aataaggctt ataaagctgg acttaaatat gaaaagattc
     9961 aattaggagg tattccaaat gaagcaggaa agaaaatggt gtttacagct gtttcattag
    10021 gaaatgagga tgtagaacaa cttaatgaac ttaatgaaaa tggggttgaa atagtcctac
    10081 aagttatacc agaggaatca tctatgacat atgaaaatgc gttaaagaaa tttaaataaa
    10141 aaatataaat taggaggaat tataaatgga atctgttttg atgttggcaa ttgtaacagg
    10201 attatggtat tggtttgcag caggtcttgc agggtatacc cttttctcaa cactaaagtc
    10261 tcctttattt ataggtttta gccttggatt actttggggt gatgtaacaa ctggtatgat
    10321 tgttggtgca agtatagaaa tggtttatct aggaatggtc gcagcagggg gaaatattcc
    10381 atcagataaa tgtctagctg ctcttattgc aataccagta gcacttcaaa ctggtgttaa
    10441 tgcagaagtt gcagtatcta ttgcggtacc tttaggtgtt ataggagtat tagttaataa
    10501 cttaagaaga actggtaatg ctgtattggt tcataaggca gataagtatg ctgaagaagg
    10561 taatacaaaa ggcatctgga gatgtgcaac actttattct ttaatatttg ggtttgtttt
    10621 aaggtttcca atagtttttg tatgtaactt ttttggagca gatttagttc aatcattatt
    10681 agatgttata ccacaatggt taatgaatgg attaacagta atgggcggta tattacctgc
    10741 attaggtttt gcaacaacaa tatttactat aggtaaaaat aaatttttac caatgtttat
    10801 tataggattt tttatggttc aatattttga aatatcaatt actgcagctg caatatttgg
    10861 tgtttgtata gctcttttaa ttacctttat gaaagaagac aaaagggtag gtgaagtata
    10921 atgcaagaaa taaaaaataa agaagttaaa aatggacaag gtccaactga agttaataag
    10981 acaagagtac ttacaaagaa agatgtaaca aaaacatatc taagatggtg gtggacagca
    11041 gaattatcca actcttttga aagaatgcaa gcattagcag tttgtgcaag tttcactccg
    11101 gctttagaaa agttatataa gagaaaagaa gatttagtag atgcgttaaa gagacactta
    11161 caatttttta atactcaagc tatatgggga ggattaattc acggtacagt tttagctatg
    11221 gaagaagaga aagctactga aggaaaaatc ccaggagaag ttatttcagg tgttaagaat
    11281 ggtcttatgg gacctttggc tggaattgga gatactttag attttggtac atttcaaaca
    11341 atattcttag ctttaggtgc ttcatttgga gcagaaggaa gtgttatagg tgcatttttc
    11401 ccaataatgt ttagtatatt actattttgc gaaggttatt acttatttca tttaggatat
    11461 tctttaggta gagattcaat taagaaaatt ctatcaggtg gtattgtcaa taagataatt
    11521 gatggtgctt caattctagg aatgtttatg atgggagcat tatctgcaac tacagttaag
    11581 ctttcaactc cgctatcatt tgatattggt ggtaaagcaa ttgttgttca agatacatta
    11641 aatatgattg ctccaggttt attaccactt ggtgttgtat tcttcgtata ttggggaatg
    11701 aaatacaaga agtggacaat aactaagtta ttagtaattt tagttgtatt agcattggta
    11761 gggtcattca ttggaatatt ctaattcaca gtgattaaaa tatagaattt aattttagga
    11821 ggagaaagaa atgtcagaaa aaatgtatac agaaaaggaa tatttagaag gaattagagg
    11881 agcaacagga gaaagagcag tatggttcta tcttcttatg aaagaagctg aaaaattagg
    11941 agctaatcca gatgatatat gtaaagaagc aatatatgga tttggtaaaa tgagaggtca
    12001 aaaatataac gtagcagata ctccaggaaa aatggcagaa atgttatata acagtaaagg
    12061 acaaaaagtg tttgaaatgg aattagtaga aaatacagat gagaatggcg ttttaaaatt
    12121 ccatcactgt ccattagatg ctgcttggaa agaatatgga ttaactaaag aagaaagaaa
    12181 agaaatttgt agattagctt gttatgggga ttatggaaga gttgactgcg cacaaggtgt
    12241 taaattagat tttgctcaaa aatgtgctca tgatgatgaa gtttgtgaat tagtatttac
    12301 aaaaaaataa tttttaatgc taaaagcctg ttctttaagg acaggcttta aaaagataga
    12361 aagtaggttg atttatggat ataagcagaa gtgtagataa tagtttaaat agaaccatag
    12421 aatttctaaa agaattgata aaaattgatt cacaacaagg tgaacctatt tcacaatgtc
    12481 cttttggtat aggacctaaa aaatctttag ataaaacttt agattactgt gctagtttag
    12541 gttttagtgt taaaaatatt gataattata ttggatatgc agaaattgga gagggagagg
    12601 aattaatagg gattccaatg catttagata tagttccacc aggagagggg tggagtgtag
    12661 acccattttc gggagcagta attgacaata ttatttatgg aagaggtgta atagataata
    12721 agggtgctgt atctatgctt atacatgtat tgaaaaacat agaggatatg tatcctacaa
    12781 ttaataaaag aataagatta atttttggta caaatgagga gacaggtatg aaatgtatca
    12841 aatattactt agataaaggt gaagaaattc caagtatggg atttactcca gatgctatgt
    12901 atccagtggt taatggagaa aaaggaaggg ttcatataag gatagaaaaa gaaattaaaa
    12961 ttgacaaaag taaaccttac ataatagtaa ggggaggaac taaagaaaat gttgttccat
    13021 ctcattgtac tgcaaaaatt attaatggaa ttatttcaga gttaactact aaaggagtag
    13081 ctgtacatgc aagtaatcct gaaaagggag aaaatgctat atctaaaatg gtaataaaga
    13141 tagttgagga taatatggat tttcagcata gagaagatat cgaattagtt tcaaaatacc
    13201 tttgtagtga ttattatgga gatgcattag ggattaatca atatgatgag gtattcaaaa
    13261 acactacctt aaatttagga atattaaaag taaatgagga aaaaatcgta tgtgagttag
    13321 atataaggta tggtaaaaat atagttttaa acaatataat agatagattc aaaaaggttt
    13381 tttgtaatgg ttggaaaata gaggttattg ctcataaaga cttacattat gtggatgaga
    13441 gtaatttagt acttaaaaaa ttattagaag cctatgaaga agtaacagat gaaaatggat
    13501 atacaattgc tatgggtggt ggcacatatg caagttggtt taaagatatg gtggcgtttg
    13561 gacctaagtt cttagcgtat aaaactggtg gtcatggagt agatgaaaga gtaccaataa
    13621 atcatatacg aaagaatatg gagatatata ctttggcttt aataaaacta ttagaattgt
    13681 aaaagaagaa gagactttat gtgcaaattt acagatatct taaaaagaga gataaaacga
    13741 gttcaacctt atttaaatgt tcaacaccat caaatggggc tgtctcaaaa tagagataag
    13801 cctcttactt attgttgaaa tgatgaaaat atattttact tcatacaaaa aagagtgcct
    13861 tatgaagtaa aaagttaatt ttgagacacc ctcatttgat gatgttgaag agaaaataag
    13921 tatgaaatta ttaaaaaaga tgattttata aaagttaagg ttgaaaagaa aagttaattg
    13981 aatggaatta gataaatgga gaaggatgta tactatctaa taaaataaca aaagagtgtt
    14041 ataaagttgg ttatatgatt agagaaaagt ctattgatac ttatccagat agtggatggg
    14101 gatttatgac ttgggatgaa aatggagaat aataaaaatt atagtttata cagtttaata
    14161 agatatatgt acaagcagtt aatctaaatg attgactgct tttcttttat agatttttat
    14221 ataatagaca aataccaata catgatagtt gcctgttata attagagaaa taaatacgaa
    14281 taataacttt ataaaagaat taaatggaaa atcgaagagt tatattcagt attttaaaaa
    14341 gaatatgaaa tgagaaatga aaataaggtg tttatattaa aagaggtttt acacaaaata
    14401 tttaaaaacg tgataaataa aaactgtatt tagatagatt aactattagt tttatataat
    14461 atggatttta tttacaaagt ttacatattt tacaaaagag attaaagata aatttaaata
    14521 tatattttat atacttgttt atttgtttct ctgtggttga ttttttcgtc aaaagtcaac
    14581 ttcaaatcgc ttgttagctt tacatgttta aggatataat tatacataag gagggattga
    14641 catgaatgaa ttaaacatcg caaaaacgct gattttaaag cgtaaagaaa aaggaattac
    14701 acaagatgaa cttgcaaatt atataggcgt ttctaaagct tctgtatcta aatgggaaac
    14761 tggacaaagc tatcctgaca taactttttt accgcaactc gcgacatatt tcaacattac
    14821 cgttgatgag ttaatatgtt atgaacctca aatgatgaag gaagatatta ataaactgta
    14881 taataaactg tgcaaagact ttacggttaa accatttgat gaagtcatgg atgaaataag
    14941 agaaatcata aaaaaatatt attcatgttt tcctttgatt tttcggatgg gtcttttaat
    15001 agttaatcac tatgatattg tagatgaaaa aaagcgtgaa ctattaatta gtgaagcatt
    15061 agaaatattt attcgtatac aggaaacttg taatgacata gatatttgcc gtcaagcaaa
    15121 aagtatggag gcaacttgtt atatattgtt aaatcaaccc atacaagtga ttgatttatt
    15181 gcaaaacagc aattttccca tgataaatga atctatactt cttgcacagg ggcaaatgat
    15241 gaatggtcaa atggatgaag cgagagaaac atttcaactt ggtgcatatc agaaccttat
    15301 atcattagtc caaaacctag tgggtatttt gcaaaatgca gataaattac aaatgaagga
    15361 aatagaaaga cgtattctag caatttcaga tatatttgag cttgatacat tatctcctgc
    15421 tattatgctt tcttcatatt taacacaggc acaaattaat ttaatacatg gtgataatga
    15481 aggagctatt aaatcattac gaaaatatgt agatttagca acacgtgata tatacccaat
    15541 tataatacat ggggatgact tttttaataa acttgacaga tggatttctg aaattggaac
    15601 aggtattaca cgtgatgata caatcgtaaa agcaggtatt gttgctgcta ttaaaaataa
    15661 tccaatgttt tctgtattaa gtgaaaacaa agaatacaaa tttcttatag aaaaattatc
    15721 tttattggag gaataagatt atggaaaatt tgatggaata cttaccactt ttggttcctg
    15781 ttattatttt agatttaata cttataatta ctgcacttgt tcatgtattg agacatccaa
    15841 attataaaat tggaaataag gcaatatgga ttattgtagt actgtttatt agtctaatag
    15901 gaccaatttt gtattttaca ataggaagag gtgaagaatg atgaatattg tctcaataaa
    15961 tggattatca aaaggttttg gtaatcgaaa gataatagat aatttaaatt ttactgtacc
    16021 agaagggtca gtctttgggt ttgtaggtaa gaatggtgca ggtaagacta caacaatgaa
    16081 aatggtactt gggttactta aaccagatag tggaactata gatgtttgtg gtgagaaagt
    16141 gacctatggg aaaacaagtt ctaatcgtca tgtaggttat ctgcctgatg ttccagagtt
    16201 ttacaactat atgagaccac ttgaatatct ttctctttgt ggtgaaatta cgggactttc
    16261 aaaaaaagaa atacagataa ggagtgaaga gttgttatca cttgtgggtc ttggaaatga
    16321 aaagagacga ataggtgggt tttctcgtgg aatgaagcaa cgacttggta tagctcaagc
    16381 tttgctttca cgccccaaat tacttatttg tgatgaacca acaagtgcac ttgaccctgt
    16441 tggtcgaaaa gaaatattag atattatgct taaaatcaaa gactcaacaa ctgtaatatt
    16501 ttcaacacat atactatcag atgttgaaag aatctgtgac catgttgcta tattaaacaa
    16561 aggaagcatt gcccttagtg gtacactttc agaaataaaa agtatgcacg gaaaggatag
    16621 acttttactg gagtttgcaa gtaatgatga aattcaaaag tttaagtcta gtgatggtat
    16681 taaatcctta ctaaaagatt cagaagaaac taatatggaa attgttttgc atggtaaaga
    16741 tataaaagca atacaaaaaa cagttatatc tacactcgca gaaatgaatc tttgtccagt
    16801 aaagatggaa ctaatagaat tatcacttga aaatttattt ttggaggtag taaagtgaga
    16861 gaatacatag catttacaaa aaaagaattt aaagagaatt taagaaatta taaattattt
    16921 agtttaatta ttttatttct aatatttggt ataagtagtc ctatttctgc aaaatttatg
    16981 ccagacttga ttgctcattt tgcaccgact ttaaaagtta ctgctgcacc aactgcttta
    17041 gattcttgga cacaattttt tagcaatatc tctggtcttg gtatgagttt aactctaatt
    17101 attttttgta atattttatc aaatgaatat tctaaaggga cacttgttat tatgctgact
    17161 aaaggtttat cacgttcatc tatagtattg tctaaattct ctgttacggt gattattatg
    17221 acaattggtt tttggttgag ttttttattt gcttatggct atacaatata tttttggcca
    17281 actgcaaatc ttaatcatat tatattttca gcatttaatt tatggcttat aggaattatg
    17341 tatattagtg tattaattct tgggtgtgta ctatttagac cagcatttgc cagtgtttta
    17401 cttgtattgg tggcaactct tttattgagt ttaatttcta ttcctaaaca gattgcacct
    17461 tatactccta atttcttgat gtcaaaaaat atagacttga tttcagggaa agtggcaatg
    17521 ccagaattta ttattccaat tattataaca gttcttattt ctgtagtatg tttactagca
    17581 gctgttattt tatttaataa aaaaccagtt taataatacc tttatcacaa ggtaaagtaa
    17641 tcttatgttg ttaaaaacta cacctaaaaa tcaaaaagac ttggacttaa gtttcaagtc
    17701 tttttgattt ttaatatata gaattaagtg tagaaaa
//
LOCUS       DAYASV010000081        17484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.081, whole genome shotgun sequence.
ACCESSION   DAYASV010000081 DAYASV010000000
VERSION     DAYASV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.081"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(12..632)
                     /locus_tag="ACGI68_002508"
     CDS             complement(12..632)
                     /locus_tag="ACGI68_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFH0138941.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(946..3084)
                     /gene="vanT"
                     /locus_tag="ACGI68_002509"
     CDS             complement(946..3084)
                     /gene="vanT"
                     /locus_tag="ACGI68_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFH0138942.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(3102..3908)
                     /locus_tag="ACGI68_002510"
     CDS             complement(3102..3908)
                     /locus_tag="ACGI68_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0138943.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(3905..5005)
                     /gene="vanG"
                     /locus_tag="ACGI68_002511"
     CDS             complement(3905..5005)
                     /gene="vanG"
                     /locus_tag="ACGI68_002511"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFH0138944.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(5266..6348)
                     /gene="vanS"
                     /locus_tag="ACGI68_002512"
     CDS             complement(5266..6348)
                     /gene="vanS"
                     /locus_tag="ACGI68_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFH0138945.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            complement(6398..7099)
                     /locus_tag="ACGI68_002513"
     CDS             complement(6398..7099)
                     /locus_tag="ACGI68_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFH0138946.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(7533..10064)
                     /locus_tag="ACGI68_002514"
     CDS             complement(7533..10064)
                     /locus_tag="ACGI68_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0138947.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(10172..10789)
                     /locus_tag="ACGI68_002515"
     CDS             complement(10172..10789)
                     /locus_tag="ACGI68_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFH0138948.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(11151..12035)
                     /locus_tag="ACGI68_002516"
     CDS             complement(11151..12035)
                     /locus_tag="ACGI68_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFH0138949.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRKFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(12226..13476)
                     /locus_tag="ACGI68_002517"
     CDS             complement(12226..13476)
                     /locus_tag="ACGI68_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0138950.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(13698..14462)
                     /locus_tag="ACGI68_002518"
     CDS             complement(13698..14462)
                     /locus_tag="ACGI68_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0138951.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(14456..15181)
                     /locus_tag="ACGI68_002519"
     CDS             complement(14456..15181)
                     /locus_tag="ACGI68_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0138952.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(15183..15551)
                     /locus_tag="ACGI68_002520"
     CDS             complement(15183..15551)
                     /locus_tag="ACGI68_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0138953.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(15727..17271)
                     /locus_tag="ACGI68_002521"
     CDS             complement(15727..17271)
                     /locus_tag="ACGI68_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0138954.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKSNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         5984 a         3082 c         1758 g         6660 t
ORIGIN      
        1 tttcgtacaa gctaaatcat tttatcatct ttatcattgt gatgtttttt atgatggaaa
       61 tcataattat gattttcaag atttttattt actctatttc caatcttcca tgacaaaaac
      121 accaataata taaatgatgc tatctttaaa ggacttgtta taaaaccttc tatatcactt
      181 gccacgtaac ttataactat aaacattaca aattttcctg ccaacattgc tggcacaaaa
      241 tcttttaaat cttttttacc aaaagctgat gcgatagtaa ctaaacaacc gggcataaat
      301 ggacaggcat acgctataaa taataactta aatccctgtt tatccatcca acttattgct
      361 ttttcagttt tactatttct caacttatta aaaagcttac tatcattaaa tctgcttatt
      421 aataagaata atgaagttgt cccaagtcct gagccaatcc atgataacaa acatccaata
      481 aataatcctt gtattgcagc atttgctgta actatagcaa caagtggtaa ggctggtata
      541 aagctttcta taaatgcact taataagccg attattgtgg ctaacaccca ataattttga
      601 gtcaaatcaa gtagttcttg tatatactgc atattgcttt tccccttttt taactttaac
      661 tattattata tactctatat gtttattata taccaatctc aaaaaatgaa tctaatatta
      721 aaaagtaaat aatctatata agtttacagt taaggtataa ttattttatt atatattata
      781 acaaaaaagg tgatgtttca aaataaaaat ctgaaacatc acctttttat aacttaaatt
      841 gtcatgtaag ttataaaaaa tatatattag acataatatt actaaaaatg tctagtcaga
      901 gtaaaataaa ttacacagta caacaaatat tactatttaa ttgagttact tctttataac
      961 atttaacctt cttcctactc tgctcaataa ttcattcgat atactacatg agttatttgc
     1021 aacttcagaa gcagataatt cagataaatt atctcttcct attattattg ccgtattacc
     1081 aacttctaca ttaggtatat ctgtaatatc aacagcaagt tggtccatgc aaattcgacc
     1141 cactattgct gctctacaac catttattat tacgtaactt tttccacagg ataaatttct
     1201 aggtatacca tcggcatagc caatagataa tatagcaatt acgctatctc tgcttgcaat
     1261 aaagactctt ccatatccaa aactatcatc tttttgtatt tttctaataa gaattacttg
     1321 tgattttaag gataaaacag gtcgtaaatc aagttgcaat ttagtcgtat catttggaga
     1381 acttaatatc ccgtataatg caattccaat tcttacataa ttacacttaa gattgggata
     1441 attaaaaaaa ccataactac tttgaatatg tactttagga atttttatac ctttttttaa
     1501 cagaaaatca attaacttat aaaaacattc aatttgttta tttgtaaagt ctacatcttc
     1561 atctttcaga ctatctgaaa cacacaagtg tgtaaatatg ccacatatat ctaagtattt
     1621 taatttaaat gcctttaaaa ttgacctcac atcattaaca tcaaaaccca gtctgtgcat
     1681 acctgtatca attttaatgt gtgattttat gttgtatttc tgtttgttta aagagatagc
     1741 atgattaagg tcaataacac tctgtgtaag attgtattta tgaagttcct ttgcccttat
     1801 tgggttagta tatccaagaa tcaatatttc tcctagtatt ccatatcgac gtaaatcaat
     1861 cccctcatct attgttgcta ctgcaaatgc ttttacacct atttggtttg cacaagtcgc
     1921 tacttcaaaa gcactatgac cataggcatt agcttttaca actgccatca attcacaatc
     1981 ttctggcata gctttttgta tcatctttac attatgttcc aaattattta aatcaatttc
     2041 aatccaagct ctattggtat ttaccttatg ttctcctttc aagtcttttt ttagaggttt
     2101 aagaatcaat ataataatta tagaagctat aaaagatgct atacttacca ctatataatg
     2161 aataaggcta ttgtcaacca agacagtttg tattccgaat attttagaaa acatacgaat
     2221 aacaacaatc atcataggat gtataatata aataattaat gatgatgttc gcaataacct
     2281 agctcgagaa cctttcaaaa atgttaaggc tgtaaataag aaatacatac atggcaataa
     2341 cataatatac atgctatcat gacgctgtac ccctagttta tgtaaaaaca ttccctcaca
     2401 taacataagt aagaatgata taagaaatcc aatcaagcta tttttagttg acatataatt
     2461 agatttatta gatattattc caccaagtat gaaaaataca ggtgcaaaaa atataccatt
     2521 tcgtgtataa tctgatactt caaaaatata cccatataaa ttctttaata gtggtatttt
     2581 ttctaaaaaa ccataataac tatcaccaaa cataccaatt acataaataa caatagtgat
     2641 tactagtgag tactttaaac ctagtctttt tacaagaaag tatgcaatac ttgcaccaat
     2701 aattgatgca ggtagatacc ataaatgata taaagtccca tcaaatacaa tgtccttaat
     2761 aatattgggc actaaattat tcatggaaaa atatccagtg taaatattta agggaatata
     2821 aattacaata gctatcccgt aaataaataa tacatttttt agaaaagatt tcagtttatt
     2881 acaattataa ttatatgttg aaattagaaa aaagcctgat agcataaaaa agaatggcac
     2941 agcaatacga gcaattatac tagttaacac aaagtcagcc attttactat atgataataa
     3001 tggagatgta tgaattgcaa ttataagtaa tgcagctata atcctaaaat aatcaagccc
     3061 tgcataagat ttattcctaa tcattcttgt tcctccattt cttagtcctt tctatttctc
     3121 caaaatgtca caataaaacc atccttattg ttacccgata tttgatatac acattgttca
     3181 ggtatagaga ttctcacttc ttctttagta tgaataggca cataaaagat ttctataatt
     3241 ttttctttat cttctatttt taaacaattt gtatacgaaa atctcttaat atattctata
     3301 tattcttcaa gagcgagatt cattttattg ataatctcag aatgcggata tcccacataa
     3361 cgaaaatgcc atggttcttg agatattcct gttatttctt cttttttaca ctgataacgc
     3421 tctataaaac cataatgtag tgctgcctta cgaaactcat tacaaatacc attataagga
     3481 aattcagggc agataaaatc tataacttct ttctttaacc ctaaatcaat agcaagccct
     3541 gtctgatgtt cactgtgatt tggaagtgct acaaactttt tagtaaagtc ttttccattg
     3601 tcacgcagag aacttgaata tatatcttgt tgttcctctg cagtacgata tccacttaca
     3661 ggtactattt catttattcc acatatactt tttaaaatat tttgtaaaat gtttgtagct
     3721 tcacgtttca taagaatact tggaaacctt gtatctacag gaattaaatc tatatcttca
     3781 gatgttttaa ctggtaaaaa agcatttact agaattagat tcccatcata aactttatct
     3841 ttagtcaata agatagtatt tgtcatttta tcttctttat ttttatctaa taaaatatta
     3901 ttcatcattt tatgtacacc tcaattatct tgtccaatat ttcttcaaaa gacaatccaa
     3961 tacctttcat catatttgga taacgactat gagaagtaaa gccaggaatt gtattgactt
     4021 cattaaaaat aatttcacca gatggagtta aaaacatatc tactctagca aatccagaac
     4081 atccaagagt tctataaata atttttgctg tctcttgtat tcttttctca caatccaaat
     4141 caattctagc tggcatatga atttttgaac ttttcaatgt atatttttct gtataatcaa
     4201 aaaatccttt tgacaattct atttcatcta ctcttccaac aagtaagtca tcgtttccta
     4261 aaactgcaca ccctacctca aaaccatcaa tattttcctc aattattact tcattatcat
     4321 gttcaaaagc caagtctact gctacttgta actcttcttt tttataaatt ttactaatac
     4381 caaatgatga acctgcacgc acaggtttta taaataatgg ataagttaag tcttttgaca
     4441 gttcagccac attaaattta tctgagtatt ttagtaatat tgattttggt actttaatac
     4501 cttctgaatg aacaagttta tgtgatttgt ctttgtccat acataatgct gaagaaagag
     4561 taccacatcc tataacctgt atacctgcca attcaaataa cccctgcact gttccatctt
     4621 ctccattttt tccatgcagt acaggaaaca caaaatcaag tttagtgtat ttaatatcat
     4681 tttcacatga ttcaatagca ctatttggaa tatgattctg agaaacagca tcaggaatta
     4741 aaaattcaat aataccattt ttgacacggt tctgcgaaac aataactgga attatatcag
     4801 tatccctttc aaaccaagtg ttattctgga tattattata atctccaata taatgatacc
     4861 aattaccctc ccttgtaatt ccaattggta taatatcata tttttccgta ttcaaattct
     4921 caagtactgc aaaagctgat tgtaatgata catcatattc tgttgagttt ccaccaaata
     4981 taactgcaat ttttttatta ttcatctttg attctccttt gttgatttat ttatttttat
     5041 acaatctaca gaaacatgtt aaaatacttt ttctatttca aatccaatca accttcactt
     5101 acaattgcat ctgttccaca taatatattt tcacattttt ctattgatat aattactgta
     5161 tcaaaataaa agcactcttg ttttgataca gtaattatat caataacaag agtgctcttt
     5221 atttatttat cctaaagaat ttcctatttt tttatactta aattcttaca aatttcctac
     5281 aatcggaata ataaccttaa agataattat attgttctcg ctatgagctg ttattgtccc
     5341 gttatgaagc tccactattt ctttagctat agcaagtccc aacccagctc taccactgtt
     5401 tgaattacga gatgtatcca acctgtaaaa ttgttcaaaa atacgctcaa gtttttcctc
     5461 tggaatcgta ttgctatgat ttataaattt aagctttata taatcatcct cctgtacagc
     5521 tgttatttca atagtagtat cttcaaaact ataataaaca gcatttcgta ataagttatc
     5581 aaatacacgt tgcattttat taacatcaca ccttatcatc atatttggtg taacattcag
     5641 tgtatatttt aaattcttac tcatcaaagt aggtttaaac tcatatgtta gttgttcaag
     5701 catacgagtt aagcttactg tactgtattc aagtgatata tttgataaat taaaccttgt
     5761 aatttcaaag aattcattaa ttaaatcttc aagacgctct gccttatcca acgaaataga
     5821 taaatatttt tttcgcagtt cttcagaaat ctgactttca tctcgaagta gtgtcagata
     5881 ccctataact gatgtaagtg gtgtttttaa atcatgtgca agataaacaa taagttcatt
     5941 cttacgttgt tctgccattt gtgtctcaaa tttacgttgt tcaagtatat gtttaattga
     6001 gtttattttc ttttctattg ccaataactc tggagataaa gccacttctc caacatcttc
     6061 tttgataaga gagtcaatac cttgatttat ttcactaaaa tattttgtaa accaatttaa
     6121 ataaacccgg aatattataa aaaacacaat caccatagct agtatcatga agagctccat
     6181 gctattacgg aaaatctgct gatataaatt caatgcagct ttatattcca tacgaaaagc
     6241 attttcaaag aatgatacta tccagataga aaacttacca tacaatataa aagaatataa
     6301 aatccatatg aaaataatgg ctataaatgc cattaaagct gttctcataa aaattttatt
     6361 tctcacttga gtaaagtcag gattgacttt aatattttta cttttcaatt gtataaccta
     6421 ctccccatac agtttttata aatttcgggt ttttagaagg ttcatgaatt ttttcacgta
     6481 atcgccctat atgagccata actgtgttat tattatcaag atatttttct ccccatacag
     6541 cttcaaacaa ttcttcagaa ggaaccactt ttccttgatg ttcacataaa taccaaagaa
     6601 tagaaaattc aatcggtgtt aatgctactt ctttcccaaa taaactacat ttatgtgttt
     6661 ctttattgat aatcaatcct ttaatatcat actcattaac aattatcgac tgttgttcat
     6721 aagaattatt atatcgcata tatcttctta attgtgtctt tactcttgca acaacttcca
     6781 aagggttaaa tggctttgtt atataatcat cagcacctat ggttaaaccc attatcttgt
     6841 ctatatcttc aattttagct gttaacataa tgataggata atagtatttt tctcgaattt
     6901 tctggcaaat atgaaatcca tcaatatcag gaagcataat atctaaaata gccatatcca
     6961 aatttttaga ttcaatacat tctagagctt ccttacctgt ataaaacttg tatactgtaa
     7021 aaccttcatt ttttaaatat acttcaatta aatctgctat ttctttttca tcatccacaa
     7081 tcaaaatatt ttcgttcata gattattttt cactctcctt attatcgagg tcatcattta
     7141 taatattggt aaatttacat atcaaataat tacaagcata tttgtaacta atttttataa
     7201 gaaaagaact actacatcag aatgtgttga tgctactatt ggaacacaaa aactgagatt
     7261 aattgttatt ataaaaactc ccctaacacg tccttattat cgcataacag gtattaactt
     7321 tcaataaaaa tatctatcag tcaaacacaa ttcaatttat tataccagag acaatccata
     7381 taaaaaacaa ttaaacctat gtatgtaacc acagacatta ctaaaatttc catgttattt
     7441 tatataaaaa ttcaaaaaag gagttgaatt taaaatttga ttagaaaaaa atcaattttg
     7501 agacaactcc tttttatttt aatgtattta aattatagta tatcagcctt cataatatcc
     7561 tcaaaatcac ctttttcaga tattaatctt atcaatttaa cagtacagct taaatcattt
     7621 ataagtatag ctccaactaa tgaaccatct ttcataaata attttttata tattctttga
     7681 ccttcatctt ttgatgaaat ttctttatca aaattttgta tattaccaat actaaatact
     7741 tttacattca ttgcatctaa tgaaactgga tatacttcac tttcaaatgt caaattatct
     7801 ccacttgcat ttgcccctgc tatttttccc atttctactg aagatggcca tattgctaaa
     7861 ctaatatttc ccacttgtgc aacatctcca caagcatata tatctttaac atttgtctgt
     7921 aatgtcttat ctacaacaat acctctatca atttttacag atgtatcctg aaccatttga
     7981 gtgtttgctc ttacaccaat tgaaaatact accatatcac agtcgacact atctccatct
     8041 ttaaatacaa ctttttgagc ttttccatca ccttcaatac catcaactgc tacacccaat
     8101 cttacatcta ttcctgtatc tcttacacat tgttctaata ttttactacc ttctttatca
     8161 agttgttttg atagtatgct atccatggct tcaactacta ctacttctaa tccttttagt
     8221 ctaaattccc atgcagcttc aagtcccaat aaaccgccac caatcactac aactttctta
     8281 gatttttcta gtgcttcttt tacactatat aaatctttta tatttctaag agtaaacaca
     8341 ttttctaaat catggccctt aattggtggt ataaagtttc tacttcctgt agctattaca
     8401 agcttatcaa atttaatagc tccatcattc actataatag ttttattcac ttcatctaat
     8461 ttatctacat tcgttcctaa tattactact atattatttt tatcatacca atctttatct
     8521 tccatgtaga aatctgaacc aagttcttca tttataccat cagaaagtgc aggtctatag
     8581 taagtcaact catcttcatt agaaatcata gttatcttac aagttttatt tctttttctt
     8641 atagcatctg ctgcataaaa accagctgct ccatttccta ctattacgaa atattcatca
     8701 gtatcttttc taaaagtaac ttcatcttct tcaacttcta taaattggtc ttccttagct
     8761 ccacatacag ggcatacact aggagcacta tcaccttcaa aaacttcatt acaaattaca
     8821 catttaaatt tcttagtttt ctttttactt ccaaaagtag aatttaactt atctaggaat
     8881 ttacctgtat aagaatataa tttatcaatc tcttcatttg atggtttaaa gttaacagca
     8941 aaaggtttta ctgctgtcat tctaagctga cttattctct ccatagcatt ttctatacct
     9001 tctccactcc atccatatga accaaatact gaagcaactt ttccaccatg aagaactggg
     9061 tttaacgata ttaatatatc ccatataggc tttaacgcat ctcctaatat tgttggtgtt
     9121 ccaaataata caccttgtga tactgctatt tttgccaaaa tatcttcttg cttatgctct
     9181 attacatcaa ataaatttac tttatagttg ctattttttt caatatatgc ttttatagct
     9241 tctgccataa tctttgtata tccatgtgca gaaacataac aaattgtaac ttctttttct
     9301 aactttattt gttcatttac actccagttg taataaaggt ctataatttt tctaggattt
     9361 tcagttaata ctggaccatg acctggacat acagtatcta tatctaaatc ttttatcttt
     9421 tctatagcag ttaccataga tggtttaaat ggacccatta tacaatcata atagtatctt
     9481 aaagcatcca agtaatcctt ttcttcattt tcatcaagtg tatttactat cttatcatta
     9541 ctatagtgac taccaaatga atcacatgtt acaagagttt tatcctcttt tatatatgtg
     9601 tatatggtat ctggccaatg aagcataggt acagagaaaa attctaaagt tttgtcccca
     9661 attgataatg tatctccatc tgtaactgca acatattcaa agtctttgtt tacgatttcc
     9721 ttaagatatt taattgctgt ttctgaagct actacttttg catttttagc taaatccaaa
     9781 agcttttcaa cgcttcctgc atggtctggt tcagtatgac ttacaacaat atagtctatt
     9841 ttttcaaaat ctatatttaa atcatttaat ctttcaatat aattgtcaaa atgcttatct
     9901 ttaactgtct caaataaaac tgttttttct gttcctttta atacatatga attataagta
     9961 gttccataag gtgtatacat aattatgtca aatactctta aatcagggtc aagcgaacct
    10021 acccaatata aattttcttt tatttttagt gatttcaatg acataccgta ctcctcctaa
    10081 aatagttatt tagtctcttt ataccctgct tgttatattt taaacaaaat ttattcaaaa
    10141 aagaaattat tttaaagata gtttaaatct tctaatcttc tttttctacc ttcaatacag
    10201 ttcccttttt agaatctact tttatgtcat attcattttt atctttatct attcttattt
    10261 tataaaaagt aacatctaaa tctttgtcta aactccattc tttaactatt ccattttgtt
    10321 cttttaatgc aatttccata gcttgttgag gtgtcattat gtcatttaaa tctaattttt
    10381 cttttctagc aactccattt aaatcttctg aatccaactt ttctgtttta tcttctaata
    10441 catcttttgt attagcatca attttcatct tatattcatt attatcatct acaccttcca
    10501 cagtataaac aaatttattt aaatctctct caagacttaa atctactata tcagcatcct
    10561 tatatttatc tttaaatatg tttattgcat ctgtgtatgg cactttaaaa tcttgttcca
    10621 ttaaattttc attatttttt tcatctttta aagcgttatt tgatgcattt gcctcctttt
    10681 ttgtattatc ttgtgcttgt cctgaatcct tagttggact cccactacat gcagttaata
    10741 aacccgctat aagcatactt accactaata aatacgtttt tttacacata aaaaccctcc
    10801 ttaaatatat taatttattc tatattatgc cctctaaacc ctttatttac ccttttatat
    10861 taaaaattcc tcctatttta tcaattttaa aactttatat ttactaaaat taaagttaca
    10921 taaaaattat actgtctcaa aatggaaaca agcctctttt tcattgttga aataataaaa
    10981 atatatttta tttcgtacaa aaagagtgcc tcatgaacta aaaagttcat tttgagacac
    11041 tcccattttt ttatattttt acttacaaaa taacagtact tattaatttt ttacaaacct
    11101 tataagtata tctatatgat attcttaaat ttaaacacta tattaaaaaa ttaaattttg
    11161 tttaattctt ttaccgttaa atccatttta ggatatggaa tcgtaattcc ttcttcgtca
    11221 aacctaactt taacctcttc tagtaaatca agataaattg tccaataatc ttctgtcttg
    11281 caccaaactc tagttgcaaa tttaatagca ttatctccat gttctactac tcctataaat
    11341 ggttctggtt catttattat taatttatga ttattaacaa tatctttaag tactcttcta
    11401 acatgtagta tatcatcttc ataccctaca ctaaaagtca aatcaactcg tctagtattc
    11461 tttgctgaat agtttattag actatcattt gatacagaac cattaggtat aagtatctgt
    11521 ttattatctg gagtaaccaa ttgtgtataa aaaagaccta tttgttcaac cgttcctcca
    11581 tgacctgctg cttctacgta atctcctact ttaaaaggtc taatcaatag tataataaat
    11641 cctccagcaa aatttgatag actaccttgt aaagccaaac ctattgctac ccctgctgat
    11701 gctatcacag cagcaaaact ggacaaactc attccccaat attccagaac aatgattata
    11761 acggcaactt taagtacact caaaagaaga gactttaaaa actttcttaa agttaaatca
    11821 atatctcttt tactaagtaa taacataaca tggtttacaa atttttttat tattttaaat
    11881 ccaatagaaa gtataagtat gcctattata agttttactc cgcttgtagt agcccattct
    11941 agcagacttt ccattatacc tcccatatcc atcttattaa tatctaaatt atttaaatct
    12001 aacttgctta tatcatctat tttcgtcgta tccataaaaa actccttacc ttatatttta
    12061 gaaccaatat tatagtttaa agttcttatt ttctatttaa tataatacaa taccatttgt
    12121 acatagtaaa caagatttat attccaatta tacatattag gaaaatgcaa actatttgtt
    12181 atatatatgc aaaatataat actaaattaa actattaata aaacactata tagccttaaa
    12241 atcatcattt tttaccccta tatgagatga tactatagtt ataatactta gtactataaa
    12301 tactacaaaa ccttttttat atccatcaac tatattatat gaatttactt gtaaagagat
    12361 agctataatc aaattaataa ttccacttcc tataaattca aaagtattaa taacactagt
    12421 tgccagtcca gaataaattt ctttattttt atacttggca tcattaaaaa cctgtagatg
    12481 tgacataact atacatccca taataaagaa tgatattggt aaaactataa ttggtggttt
    12541 tacctggtat acaactacaa ttacaatcca tataaacaaa tctatcattg ctccaaattt
    12601 tattatatta aatttgctag accttatttt agcaaataca aaatccataa ttattgaacc
    12661 aaatataaat ccatatgtaa aaaatgatac tataaacgca gaaaaactct tactcacacc
    12721 ataaacgtcc attatgtatc ttactcccca caagcttata aatgcacttg taagaccaac
    12781 aaaagagaac attatcattg aattatacca tgttgatttg ttctttataa cagacttaat
    12841 accatcaaca atattaacct tctctgactt ttcatagggt tcagtatcta cattgaatcc
    12901 atactctttt ggtgtatctc taacaataat atacataaaa catccaatta caacaccaat
    12961 tataccgatt aataaaaatg aatttctcca accaacaagt tcagacaaaa acacaagtgg
    13021 aaaagtagca agaactccac ctaaatttcc tacaaatgaa aatttagctg ttgcagaagc
    13081 aaattctgac ttattaaacc atctcccttg aatttttaaa atacaaagta atattactga
    13141 tgttcctgcc cctacaatta cacgagaaat atatgctaat tctatacttt gaatcaaacc
    13201 aaataaaatt gaccctaaac ttgccattat tattccaaga ctgctaattt ttcttgctcc
    13261 atatttatca atcaaaattc ctgcaggtat ttgcataaat gcatatgcat ataggcaaaa
    13321 ggatgctata tttgaaattt gtatagaatt aaaacctaac tcatttgcta aattgtctga
    13381 aacaacagca gttgacatcc ttaaaaataa tactaaaaca aatgaaaatg taattatacc
    13441 ccaaacactc cacttagcct ttgtataact tttcatatct tctccctcaa atccatatat
    13501 ttttaaattt tcttgatata acttatataa agtgtatagt tattcttaca aatttttaca
    13561 cgcaatatca ttatacaaca tatgtatata tttaactaga aataaaacac tattcatatt
    13621 atatacatga attaactaca taaataataa gagccatctt ttaacattac tcaaaagata
    13681 gctccatata aaaacattta ttctaaattt aattttttat ctaatataaa ttttgtagcg
    13741 aaaaataacg ctattgcaac tacaagacta ccaagtattg caagcataaa actaggactc
    13801 tgataaagat gatataccat atttgtatct tcatttggta agatgcttcc aactgcatct
    13861 ccaacaattg atattactat atttatagct ataaaagaag caaatgccac tatgtttcta
    13921 tgtttattga attttggcaa ttgtcccatt gagattgatg tatataataa caaaataaat
    13981 gatgaatatg aaataaacat tactactact aataataaaa tatctaaaaa gttttcagaa
    14041 atccatttaa aactagtgtt aaacaaatct aaaatttcag gtagtaaaat tccagaagta
    14101 ccaaaaagca taagaactcc aaaagtgaat actgcaacta taaaactaag tacagcgtat
    14161 ataagtgctg ttatacattt agataatata agtgaactag tgctaacagg aagagtaaac
    14221 attaaataac cttcatcccc caaaagattt tttctaaacc tttgaattgt aactacaata
    14281 gttaaaactc ctaatgccat aaaaagactt gttaatacta gtattcctat tccctgtacc
    14341 tgaagtatat ttgtattcat aaatattcca ttaaatacag ctactatcaa tatggctata
    14401 tataacggta taaatattct tccacttgct tttaactcat attttaataa tttacctaac
    14461 atttaaattc ctccctaaat aatgcatcta tagattttcc tttttcttct cttattgctt
    14521 caacgtctcc ttgtaacact atttctcctt ttgatatgaa tattatctca tcacatatgt
    14581 tttctatttc acttattaag tgggtagcaa tcagtaaagt actatcttca gaataatttt
    14641 ttaatatagt cttaagtata tatgaccttg cagctgggtc aacaccacct ataggttcat
    14701 ccaatatata tatgctcgca tttcttgaca ttactaaaat aagttgaact ttttctttag
    14761 ttcctttaga cattgtcttt agcttttcat ttacatcaat atctaagctt ttaatcatct
    14821 catttgctct tctaacatca aaatcactat aaaaatcata gaaaaactta agtaaatctg
    14881 ataccttcat ccaatcattt aaataagttc tttctggtaa atatgaaact atttttttag
    14941 tatctatgga tggtttcatt ccatttatca taatttcacc attatcaggc tgtaataatc
    15001 cattcattag cttaatcatt gtacttttgc cacttccatt aggtcctaac agcccgacta
    15061 tttttccctt tggtatattt aaatcaatat tttttaaaac attcttagtt ccataactct
    15121 tgtttacacc tttaaattca actattccat cttgagaaac attgcttata tttttatcca
    15181 ttttataatt ctcctttcag attttctgta ataagtttta gcatttcatc tctatcatat
    15241 ccaagctttt ctaaggttac ttttaatgta tttatctcca cattagctat ttcctctttc
    15301 atagctttta ttttttcctt atcatctgtt acaaatcttc ctttagttct ctgactataa
    15361 actaaacctt gtctttcaag ttctgttaga gccttttgca ttgtatttgg attaacttcc
    15421 gcatcttctg ctagtgctct cactgtttct aatttagatc ctattttaat ttcccctgaa
    15481 attattttta actttaaatg ttctactaat tgtatatata taggtttgtt attatcaagt
    15541 tcccattcca tgttcatgcc tccttattat attattgtat taagttgtta atacaataat
    15601 ataataatac aaagtatatg tcaattactt tttagtaaaa gtataaaaaa ttttacaaaa
    15661 aaaatgaaca ctacattaga gtccattttt aacacaattt attaaattct ttatattaca
    15721 gattaattac cctcttacca agttagatac aattaaatgt tcaaattctt caattggcat
    15781 tggctttcca aacaaatatc cttgtgccat atcacacttt acttctttta aaagtttaaa
    15841 ctgttcttct gtctcaactc cttctgatat gactttcata cccaactctt ttgccatttc
    15901 tattacttta gtaacaatta ttttgcttct tttactatta taagtttcca taataaattt
    15961 ttggtcaagt tttaagatat ccataggaat ttcttttaac atatttaatg atgagtaccc
    16021 tgaaccaaaa tcatccatag atattaaaaa tcctattttt tttaaattat tcattatatc
    16081 aattaaaata tcaaaattat caaatatgat actctccgtt aattcaagtt ctatatactt
    16141 tgcaggtata ttgtatttat caattatatt tttgtatgtt tctataaaat tattgcaata
    16201 taaatgtatc cttgaaacat taactgaaac agtaactgga tttattcctg catcaatcca
    16261 ttttctaagg tatatacata cttcttcaag cacatataaa tctaaattta taacaaaacc
    16321 gtttctttca aaaaatggga taaattcatt tggaggtatt agccctttat caggacgttt
    16381 ccacctaaca agtgcttctg aaccttgtat ctctcctgta ttaagttcta tcttaggttg
    16441 taagtatata ataaattctt tattttctag acttgaaaac attgtatttt ccatctcttt
    16501 ttcttttatc tcttggtcat gaatcttatt atcataaaat gcatatgaac ttttatgtcc
    16561 tccctttgcc attttatgag gtatattagc cctatctatg attgagttga tatctctatc
    16621 ttctggaagt ataaaataga ttccacaact taaaactaac ttatattttt cttcttgatt
    16681 attattaaac aagtctaatt cttcaaatat agtttttaac ctctttgtta tatcttcttc
    16741 ttctatatat ttcataatta taataaaatt atccgcagat attcttgcaa acatttcctc
    16801 ttcaaatata ttgctcttaa taatctttgt taaattacgc aaaatttcat tcccaacttc
    16861 atatccaaat aaatcattta tatatttaaa tttatcaata tctatatata ataaagcata
    16921 ctttacttga gtatttttac atagtatttt gttagcatca attacaaatt tatctatagt
    16981 atttgctcct gtaagattat ctgtatatgc tatcctttgc aatttaattt taaaatttaa
    17041 tttatcatat attatataga atataagtat tacacatatt atagccataa taagtattat
    17101 ttgtattgca tgtttgctaa taagctgatt attagacaca atagactcaa agttatttat
    17161 ggagttgttt ttatagagtt tgcataaaga atagactggt gttgtgtatg taatttgtaa
    17221 aattatacaa ataaataaaa aaaacatcaa ttttttttgt aaattcctca taaaaatttc
    17281 ctcccattaa aacacctcta ttttattagc aaataagtac atctcaaaag tttccatttc
    17341 ttgtaaaatg gatagtttat atctacttta attttgtaaa attttctgct actataatta
    17401 tagaatgtag catattcatt gtcttaacta ttgatttata tatttcatat aggtataata
    17461 gaaaataaat tggattattt tggc
//
LOCUS       DAYASV010000082        17450 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYASV010000082 DAYASV010000000
VERSION     DAYASV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.082"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            128..349
                     /locus_tag="ACGI68_002522"
     CDS             128..349
                     /locus_tag="ACGI68_002522"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138955.1"
                     /translation="MKNVLKSNRKSKKLTQNQLSKLANISQSYISDLEKEYFVHSPTV
                     KQIISLSKALSIEPCELAEYFIKKEIESK"
     gene            365..514
                     /locus_tag="ACGI68_002523"
     CDS             365..514
                     /locus_tag="ACGI68_002523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138956.1"
                     /translation="MEKYFLDELNKLNNPTISEIIEVSEKLSLSPFIIASYFIEKEEE
                     SSSSI"
     gene            complement(1110..2309)
                     /locus_tag="ACGI68_002524"
     CDS             complement(1110..2309)
                     /locus_tag="ACGI68_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138957.1"
                     /translation="MDSQFTVIHNLKPITRMSKSKLKDMLGIFKTSTRKIEKTDRSVC
                     NSCNIRKNYSLHEDRLDDFCSNCPKIKTFTEFSYVIEKETNTIGTIEIVTNENKTISL
                     SKSAIKQFIAYHFIPCNKNFVRKSVSFKNIADICNVSVVTARYNHSILIEAGLVYSTK
                     VKRGKFDIVIDGEYKNHLKKSDGGQGYITMSLDTLKHLLKFDNVNELKLEIKKLLWAD
                     ANTSKGKKVKFNKENLISVLPSYLRKSSKRLEAFLKGSKTLFNISNNRIDTSRYETKE
                     KVRNRYINFIKEKIEKFFINTGCSLSANYSKVLEQVNLDKHELYKEDLNRQLELEKEL
                     IINDLVGLSIQYSINIVLKTLNYVFNEYTIDENDINYSEIKNPGAFIRTIIVKNINKN
                     GSLKIVI"
     gene            complement(2698..3102)
                     /locus_tag="ACGI68_002525"
     CDS             complement(2698..3102)
                     /locus_tag="ACGI68_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138958.1"
                     /translation="MKLKEVTPFGKILRKIRIDNDETLKDMSEKFNVTSSHLSAVETG
                     KRSIPKQWQDIIVKEYNLNENETNQLKKSILHSATEVKINTIDLNKDEKELVFAFASR
                     FKHLNSQDKEEIKSILKKIDSKELSGFSTRND"
     gene            complement(4441..5340)
                     /locus_tag="ACGI68_002526"
     CDS             complement(4441..5340)
                     /locus_tag="ACGI68_002526"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0138959.1"
                     /translation="MVITLSQNEDKYKVIQSLFNEKKASLIKLCEIAQVNRSGYYKWL
                     NRDKSNLELENIKLATLITKIYEEKNGVFGSLRMTLQLNREYKLNVNHKRVYRLMRAI
                     GLKSVCRKKKFSYVKCTPEVIAENVLSRKFSADKTFQKWLTDVTEFKLTNGTKAYLSA
                     ILDLGDRSIISYVIGTSNNNNLVFETFDKAIELYPNAKPIFHSDRGFQYTSKVFKSKL
                     LAQGMIQSMSRVGRCIDNAPMEGFWGILKVEMYYLRKFDTYEQLVKAIENYIYYYNNF
                     RYQKRLNSMSPLEFRKYLSTEIA"
     gene            complement(5334..6005)
                     /locus_tag="ACGI68_002527"
     CDS             complement(5334..6005)
                     /locus_tag="ACGI68_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0138960.1"
                     /translation="MSKTKISSEQKIKAIESYLKGENSVIKIMGELNIGKRTFREWMR
                     NYETFGNDGLIKQSRKSYYSKEFKLSAVKAYLNGEGSYDTISKKYKMRSVHPLKAWVK
                     EYNIHKEIKETPKGGLFMETRKTTLDERVVIVQDCIEHDFNYNLIAKKHSVSYSQVRR
                     WTLKYKDKGISGLEDRRGKTKKESELTQLDKVKLELKILKAEKHKLQLENEFLKKLKN
                     IERGW"
     gene            6685..6930
                     /locus_tag="ACGI68_002528"
     CDS             6685..6930
                     /locus_tag="ACGI68_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138961.1"
                     /translation="MIKFLVGKTYETSSICDRDCKFKIKIISRTEKTLVYEDEDGNRA
                     RTKIHKLEDINTEFIRLGNYSLAITFKADNNLINDCI"
     gene            6947..7354
                     /locus_tag="ACGI68_002529"
     CDS             6947..7354
                     /locus_tag="ACGI68_002529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138962.1"
                     /translation="MYNSNINNRLIEINTNKCTLKNIEIVLMEGSKDYIKNFKIIYTK
                     LLSLQLQSVSLQYFGNNIILITDTYSKTLELLDEELLKQYLHIKHLSSDNFDSDILEN
                     CKLESYKINKHLISNRIFRLIKNEIDCISLKNL"
     gene            7372..7701
                     /locus_tag="ACGI68_002530"
     CDS             7372..7701
                     /locus_tag="ACGI68_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138963.1"
                     /translation="MKKNKISFRKWFKFYLIGCSCICIIVSLFMLIYFGSNRIETMET
                     HSAYNFIESKIPTNAKYQGYKKNHINAKTVLYYSYKDSIHTVELYHPENNLNEVDWNE
                     VTDIKFD"
     gene            complement(8065..8595)
                     /locus_tag="ACGI68_002531"
     CDS             complement(8065..8595)
                     /locus_tag="ACGI68_002531"
                     /inference="COORDINATES: protein motif:HMM:NF037233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138964.1"
                     /translation="MSDKITNRLCFLGEEKEIKEILEFIKNDEIGVGSIDFNKIISMP
                     SWIYKGSLSKNECKKHRDENCWLEWCMKNWGTKWNAIGGIDKRSTDDTIYFFTAWSNV
                     TQIIIKLSLIFPTVEIEYSYCNEEFGTGVGMIKFRNTRIIEEYIPNDYSKEAYEGSLE
                     IEKSKPEDYNIIFDEN"
     gene            complement(8667..8963)
                     /locus_tag="ACGI68_002532"
     CDS             complement(8667..8963)
                     /locus_tag="ACGI68_002532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138965.1"
                     /translation="MEYIIHNKFDTNKFNIYKAIRLEYFDIAGWYKFSRDVLITNINE
                     TEIKFMYAVKDWNGLATSFGVVKVEDILREYIKIKFLEVLDNKKSDGRSNQHNK"
     gene            complement(8992..10176)
                     /locus_tag="ACGI68_002533"
     CDS             complement(8992..10176)
                     /locus_tag="ACGI68_002533"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFH0138966.1"
                     /translation="MKELIIDNFAGGGGASEGIELATKRKVDIAINHDPSSIRLHKTN
                     HPNTKHYCENVWDVDPVEATQGQPVALCWFSPDCKHFSRAKGKTPVDKKIRGLAWVAV
                     RWASKVKPRVIILENVPEFKTWCPVKNDRPVKSKKGETFHKFINALERLGYKVEYKEL
                     KACDYGAPTTRKRFFLIARCDNKPIVFPEPTHGENKIPYIPASEIIDWDIEVPSIFNR
                     KKPLSENTLVRIARGINKFIIEDKPYIVNKSAYFLTHYYTHQGLETRGSSLRKPINTI
                     PTANRFGLVRVDFNDIRLPDNEELYEFLKYYGSEIGQSLNRQLQKINDIGLRMLTPRE
                     LFRGQGFPESYVIDRDYKWNLYPKTEQVARCGNSVPPILSKVLVESNLPELCGISYSN
                     AI"
     gene            complement(10242..10817)
                     /locus_tag="ACGI68_002534"
     CDS             complement(10242..10817)
                     /locus_tag="ACGI68_002534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138967.1"
                     /translation="MNIAKSKVKKCIRETIEVTSIENLKCCGFYIIEDKIHHYIHCEG
                     QTLDENIYNGEYDYLYDYDDIIRIYNQKKFTLKDIDEKVFDKIQEMINKKEKYDTTIA
                     MFIKSIKEINNKKLQICKFNGKVKKLYREYIGNFKKWSEFYEEDITEYKSHIYDLEEI
                     NLFLKVDFELINENKENLLKSTIKLYGIEIF"
     gene            complement(11073..11405)
                     /locus_tag="ACGI68_002535"
     CDS             complement(11073..11405)
                     /locus_tag="ACGI68_002535"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0138968.1"
                     /translation="MTEKIDELLNSKNITKSQLSKESGIPYTTISGFYVKGTDNVKLS
                     TLKKLADYFQCSLDYLVYDDVGLNENIVFNGYINMFAKLNREEKELTYKAIRILYKNK
                     EGINYIGN"
     gene            complement(11445..11984)
                     /locus_tag="ACGI68_002536"
     CDS             complement(11445..11984)
                     /locus_tag="ACGI68_002536"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138969.1"
                     /translation="MSEKDGIIRLNLIELRKILKLTQEQMSASLKISRSNYGNIETGK
                     VGLTERVKSDICDIYNVNKEWLETGTGEIFSKESNCGKKINELRKNRNLTSEQLSELS
                     NVPKGTLDKILNGTTKDPKFETLKSLARALNCTLNDFDDMNTINIDIKAKEFNYLFAQ
                     IDNETKDLIIGIMKKVLNN"
     gene            complement(12120..12500)
                     /locus_tag="ACGI68_002537"
     CDS             complement(12120..12500)
                     /locus_tag="ACGI68_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138970.1"
                     /translation="MSDETISTRIRKIRKDANLSQPAFGEKLSVSKDVISNIEYDRVE
                     PKPLLINHLCSVFNINKEWLINGTGDMYVSVEDDIYSTLGEVFYKIASSKDENLKELI
                     INISKLDATYIASLNEIVKRSIQR"
     gene            complement(12520..12888)
                     /locus_tag="ACGI68_002538"
     CDS             complement(12520..12888)
                     /locus_tag="ACGI68_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138971.1"
                     /translation="MIDIDTNKRIKEIREDINLSQQKFGEKLGVSRDVISNIENNRVE
                     VKTVFITHMCEVFSVNQVWLETGEGRKFIEQECDIVLGEVFANITVSKNENLKELIIN
                     ISKLNDTYINNLNEIVKGLI"
     gene            complement(12899..14344)
                     /locus_tag="ACGI68_002539"
     CDS             complement(12899..14344)
                     /locus_tag="ACGI68_002539"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138972.1"
                     /translation="MQEELNIIPFLAETLSTNKKCYKLIDKYYNENKLKYSNLAKENI
                     FYDSRIASEGSIIQEYYFKKSLGILLEKDEKKITRIFKVGYTIAYNYIISVPIFKAST
                     FLRKLMLKNKMEKFSDDELNGNMLVAISLCGALEKEVDESDEIYVKVIKNLILRNESY
                     KCEKFFSIDKLSKEEQKLISKIELKLKSIYLKEYTPSSYAIRVNNESTGTFKIDDLSY
                     LDRNLLCFDYVYDLEAISVISIVGKDIFKSKQIQELILCYCSLQNNIEDENSINYEDL
                     LRFIIPAIDLRYLLVEYKKAKQFFFNNFDEELKESIEEKDIELITLKKENSLLREENK
                     NIKLELELLQKDKLRLENEIKAQITSKEELVQLRNFMFSKQQEYIENIPTDERDLKNI
                     KAIIFGGHPNWILKMKDKLINFEFISAETINFDVNILNSYEYVFINTNYVGHAMYYKI
                     IENLNENNKLRYINNINIDRAIRDIKDAIKI"
     gene            complement(14447..15046)
                     /locus_tag="ACGI68_002540"
     CDS             complement(14447..15046)
                     /locus_tag="ACGI68_002540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138973.1"
                     /translation="MKRITKKEIKKYVREATSNNFNWKLSRCGFYIKNDKVQFFISYV
                     GQGMDENIYNNDYEEIIYIEDIIEEYRRKEYNLEDIDTIIYENVNNMIYNYNERIEDI
                     EDFIQEIKDSIGEEFTIFEYDNFVQGKHNHLSDKLDSWDYFTEGDINDYLESGSYTYI
                     FFYEDLNRTVNLNIGFEVIERNEEEICESKIKIREIILL"
     gene            complement(15311..15466)
                     /locus_tag="ACGI68_002541"
     CDS             complement(15311..15466)
                     /locus_tag="ACGI68_002541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138974.1"
                     /translation="MYLVESIQILNFDIISYENCYVDEKYVLSENFIKNMNIVLDKIE
                     NIISFYE"
     gene            complement(15636..17285)
                     /locus_tag="ACGI68_002542"
     CDS             complement(15636..17285)
                     /locus_tag="ACGI68_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HFH0138975.1"
                     /translation="MDKKEEILKEIKKDPYFLKYVKVQTEDMCLLAVRYIGWTLEFVH
                     NQTEEICKEAVKQNGLALKDVKEQTEEICLLAVKQNGRALKYVHNQTEEICKEAVKRN
                     GLALKAVKEETEEICSLAVKQNGDALKYVKKQTEVVCKEAVKQSYEALQYVKKQTEEI
                     CIEAVKQNYKALQYVKKQTEAVCIEAIKQNRKAFELVDERTEAIYIELVRQNGLDLEI
                     VDEQTEAICIEAVKQNGKALQYVKKQTEAICKEAVRQNGRVLFYVEEQTEEICLLAVK
                     QNGFALKDVKEQTEEICKEAVKQEGGALEYVKKQTEEICLLAVKQYGGALKHVIKQNY
                     EICKEAVKQSGWSLFYVKEQTKELCSIAINNKAYALTSVKEQTRELCLIAIRKEPLTL
                     KYVREQTEEMCLEAVKRNGMALKFVDKRTEEICIEALKQDKHSILYIDDKEKYLKMFS
                     DDIKFLNSLEQLKRDNKYKFAFSNIEFKEAEEEIQNIIAIKENGKWLFTLCNLFYLDD
                     GWKDMSKGRFIYKIYNEEGGYNIHHRVNLYRQIYLEFLEKF"
BASE COUNT         5851 a         2611 c         1650 g         7338 t
ORIGIN      
        1 tatttatttt tctatttttc gacatataat gtaagagggg gtgattaatt gttgaacaaa
       61 acattaatgt atatttaaat aaatatgtat gtatacaaat ttaaatatgt tttttcagca
      121 taataatatg aaaaatgtgt taaaatctaa tcgtaaatca aaaaaactaa ctcaaaatca
      181 gctgagtaaa ttggctaata taagccaaag ttatatttct gacctagaga aggaatattt
      241 tgtacatagc ccaacagtaa aacaaattat atcactctca aaagcccttt ccattgaacc
      301 atgtgaatta gcagaatatt ttattaaaaa agaaattgaa tcaaaataag ataggaggta
      361 tattatggag aaatactttt tagatgaact taataagtta aataatccaa ctattagtga
      421 gattatagaa gtatcagaaa aactatcatt aagtccattt attattgcat cttatttcat
      481 tgaaaaagaa gaggaaagta gcagctctat ttaaaagttt tttctaaact gtatatataa
      541 aataagtatt tattaatact tttgagataa tttttctgta gtaagaatat tttataaaaa
      601 aagataaaga atagtacttt tgatggttac aaaggtacta ttttttaata aatataagta
      661 atattgttat atacatgaag ttatatacat gaaattacat acatgaaatt actacagtaa
      721 ttacgtacag taattagcaa atgattgtaa tagaagaaga gtagtaatta caatatcttt
      781 aagaaatata aatttaaaat atttaaaaac tgtatataaa tagtttttgt ttatttataa
      841 tttattttat ttttaatata aataaacttt gcttatttat aatttattta tatattttga
      901 taataaaatt tttattgtaa ataaaaaact ttctattgtg tataatttta atataaaaaa
      961 acaccaaaaa tagaactaat agaagagttt tcattctaag tagttctatt tttgataagt
     1021 gaagatttga atataaacac taataggagt agccaaaatt aaattttttg aactactccc
     1081 tattattata gatttttata ctactaatat catattacta tttttaagct tccattttta
     1141 tttatgtttt taacaattat agttcttata aatgcacctg gattttttat ttcactataa
     1201 tttatatcat tttcatcaat tgtatattca ttaaaaacat agtttagagt ttttaacact
     1261 atattaatac tatactgtat agataaacca actaaatcat ttataataag ttctttctct
     1321 aattcaagtt gtctatttaa atcttcttta tataattcat gtttatctaa attaacttgt
     1381 tccagcactt tgctataatt agcacttaaa gagcaaccag tattgataaa gaatttctct
     1441 atcttttctt taataaaatt aatatatcta tttctaacct tttcttttgt ttcataacga
     1501 cttgtatcta ttctattgtt acttatatta aataaagtct tagaaccttt caaaaaggct
     1561 tctaaacgct tacttgattt tcgtaaataa cttggaagaa cagatataag attttcttta
     1621 ttaaatttaa cttttttacc tttacttgta ttagcatcag cccaaagtaa tttttttatt
     1681 tctaatttta actcattaac attatcaaat ttaagtaaat gttttaatgt atctaaagac
     1741 atagttatat aaccttgtcc accatcagat ttttttaaat gatttttata ctcaccatct
     1801 ataactatat caaatttacc ccttttaact tttgtagagt aaactagtcc agcttcaatc
     1861 aaaatagaat gattatatct tgctgttaca acagaaacat tacatatatc agctatattt
     1921 ttaaaagaaa cagattttct aacgaaattc ttattacaag gtataaaatg ataagctata
     1981 aattgtttaa tagctgattt acttaaagag atagttttat tttcatttgt tacaatctct
     2041 attgtaccaa ttgtattagt ttctttttca attacataag aaaattctgt aaaagttttt
     2101 attttaggac aatttgaaca gaagtcatct aatctatctt catgtaaaga atagtttttt
     2161 ctgatattac atgaattaca aacagaacga tctgtttttt ctatttttct tgtagacgtt
     2221 ttaaagatac ccagcatatc tttaagctta gactttgaca ttctggtaat aggttttaaa
     2281 ttatgtatta ctgtaaactg gctatccata ttgagatacc tcctttatat gtgtaaaaca
     2341 tataaaaaag ccttaatatt attatatatc aaggctttga gacatcatat ttacagtaac
     2401 ttccataaga tttaatctaa aataagcacc aaaagtattg caagtaatca taaaatatga
     2461 tattataatc ataaattatg aagtaaatga ggtctacatg ggcacatgat aattaatatt
     2521 tgcgcttcta ctttaaagta ttaattgtca gattgataag tgaattggcg ttccttatca
     2581 atcctgtgag ttgtaggctt tttttatttg ttttattata ttatatttta tattttaatt
     2641 ttttgcaatt atttttatat taatatttaa tattatcagt ttttgtaact atgttattta
     2701 atcattccga gtagaaaaac cactcaattc ctttgagtct atttttttta aaatagactt
     2761 aatttcttct ttatcttgac tatttaaatg tttaaaacga cttgcaaaag caaaaactaa
     2821 ctctttttca tctttattta aatctatagt atttattttt acttccgttg cagaatgaag
     2881 tatagacttc tttaattgat ttgtttcatt ttcatttaaa ttatattctt ttactattat
     2941 atcttgccat tgtttaggta tacttctttt tccagtttca acagccgata aatgagatga
     3001 tgttacatta aacttttctg acatatcttt aagtgtttca tcattatcaa ttcttatttt
     3061 tcttaatatt tttccaaatg gtgttacttc tttaagtttc atatacatca tcctccttat
     3121 atatctatat attaccatta ttacaatata aattcaacaa taattgatta ttttttttgc
     3181 aataaggata ttttaacttt taatactttt attggccaac aatggttatc cataaaaata
     3241 ttaaaaatac tagtttttat aggtaagaga gtagtcaatt atggtataat aaataaaaga
     3301 aaattaattt ttatacaaat tttaataatt tactcattaa aaaatcactt ctttacagct
     3361 gataaagagt gattttttta atgaatattg taggaccaga tgaatttata tttaatgtat
     3421 tgaaataact taatttttat atgcaaaaac gtaacttttt taattttttt aaataatatt
     3481 tctatattta atacttaaat cgttctgatc taaacgaaat tattatttaa aatgaaaaat
     3541 aatttcgttt tttgcaaagt actatttatc ttataagttt atttttgagt taatattttt
     3601 aataatttat tcctactagg tatatctaag ttttaaatat ttgataaata aatatttaaa
     3661 acttacaaaa aaagaaaatg gactatatcg aaaacacgac tagcccaaac acaaaatatt
     3721 gctattattg ctatggtgat aaacaatctg accaggacat tttaaccata gacttttaca
     3781 ttaacgagta caatgacaac aacacaatac taaataacat attagatatt ggttatatca
     3841 agatacaacg aaaaaattct tctatttata aattatataa taaatttgtt caaaatgcta
     3901 gatattttca gtagagtaga gtccaaaaat tattaattat tctttttgtg tagtgtataa
     3961 taaatattgc ttttctggaa cttaatatta agttccaagc cattcttagt tagtagctta
     4021 gataaaataa actactagac tggcaattaa actctctaat acacttagta aatagcaact
     4081 tatatttttt ctaaattaat aaaaagatat aaatttgtat caaaatctac attttgtggt
     4141 ataataaaag caagaaggac tatactttat ttgctgtaga gtagagtcca gaaattattg
     4201 attattcttt ttgaacttaa cttttagttc aaaactaaag ctactctttc gtgaactata
     4261 cccactgttt tatacagtaa aaaaatgtaa aagtagttat aaagtactag tgtcactcat
     4321 tagtactttt ttattatatt aatataatga atatatatat tttaaagttt actatctaaa
     4381 aaagagcacg tcaaataaaa ctctagatat ttaaattttt ttagagctac ttaaaataaa
     4441 ttatgctatc tctgtactta aatattttct aaattcaagt ggagacatag aatttaatct
     4501 cttttgatat ctaaaattat tatagtaata tatataattt tctattgctt taactaattg
     4561 ttcatatgta tcaaatttcc ttaaatagta catttccacc tttaaaattc cccaaaatcc
     4621 ttccatagga gcattgtcaa tacaacgacc aactcttgac atactttgta tcataccttg
     4681 tgctaaaagt ttcgatttaa acaccttact agtatactga aaacctctat cactatgaaa
     4741 aataggcttt gcatttggat atagttctat tgctttatca aaagtttcga acaccaagtt
     4801 attgttattc gatgttccaa taacatatga aattatactt ctatcgccta aatccagaat
     4861 tgcacttaaa taggccttcg tcccattggt taatttaaat tcagttacat ctgttaacca
     4921 tttttgaaat gttttatctg cactaaattt tctacttaat acattttcag cgattacttc
     4981 tggtgtacat ttaacataac taaatttctt ttttctacaa acagacttta agcctatggc
     5041 tctcattaat ctgtacactc tcttgtgatt gacattaagt ttatactctc tgtttaactg
     5101 cagagtcatc cttaaagagc caaaaacccc attcttttct tcatatatct tagttatcaa
     5161 agttgctaat ttaatgtttt caagttctaa attgctttta tctcgattga gccatttata
     5221 gtatccagaa cgattcactt gagcaatttc acataatttg ataagcgaag cttttttttc
     5281 attgaataat gattgaatta ctttatattt atcttcattc tgggatagtg taattaccac
     5341 cctctttcta tgttttttaa cttttttaaa aattcattct ccagttgtaa tttatgtttt
     5401 tccgctttta gaatttttaa ttccaacttt accttatcta attgagtcaa ctcactttct
     5461 ttttttgttt tgcctcttcg gtcttctaat ccagatatcc ctttatcttt atatttaagt
     5521 gtccatcttc taacttgact gtatgaaaca gaatgttttt ttgctatcaa attgtaatta
     5581 aaatcatgtt caatacaatc ttgaacaatt acaactcttt catctaaggt tgtttttcta
     5641 gtctccataa agagccctcc cttcggggtt tcttttatct ccttatgaat attatactct
     5701 ttaacccaag cttttagtgg atgaacagat ctcattttat atttttttga gattgtgtca
     5761 taagaacctt ctccgtttag ataagccttt acagcactca acttaaactc tttagaatag
     5821 tatgatttac gactttgttt aattaagcca tcatttccga aagtttcata attacgcatc
     5881 cactctctga aagtacgttt tccaatattt aactctccca ttatttttat tactgaattt
     5941 tcacctttta aatatgattc aattgctttg attttttgct ctgatgatat tttagtttta
     6001 gacataacaa aaccctccta gttttagaca gttttacata ttttaactgt ctcctaagag
     6061 gggtatagtt catcgcacag agtggctttt tacttttttg tttaacttac taattaagta
     6121 aattattaca ctagctgcta aacttgctaa aacactaagt aaaaactcgt tcatacttcc
     6181 acctccttcc ttttaggaag ttggttttta tctttagtat gaactccact cttaaaatat
     6241 agttaatctt cttgcttcta taattatatc ataaagtttt ggaaattaat aactatagtt
     6301 catataatac aaatctattt atttcaattt taaaccctgt aaatgcaata agtaatgtaa
     6361 ttatactttc acaacctaaa aatctctata aaccaatcta agaactttta taaatataat
     6421 gtatttattt aaagtcaaaa tcagtaaacc ttcaaaataa gtaatgtaaa tatttttagt
     6481 cgttttttta tcgcaacagc ttatcatatt tttttatttt gtcaaaacca gtataaattt
     6541 tttttagccc aagaacattg gccaaaaaaa atttatactt agcaaagctt tttgacaaaa
     6601 taatgcaaat atgaaatctt cagctcaaca aaaaaacaac tcgacttttt aatttttata
     6661 attaattttt atggaggtaa gatgatgata aaattcttag ttgggaaaac ttatgaaaca
     6721 agtagtattt gtgatagaga ttgtaaattc aaaattaaga ttataagtag aacagaaaag
     6781 acacttgtat atgaagatga agatggaaat agagctagaa ctaaaattca taagctagaa
     6841 gatataaata ctgaatttat aagactaggt aattatagct tggctattac atttaaagca
     6901 gataataatt taattaatga ttgtatatag tggaggagag agcgatatgt ataattcaaa
     6961 tataaataat agattaattg aaataaacac aaataaatgt actttaaaaa atatagaaat
     7021 tgtattaatg gaaggttcaa aagattatat taaaaatttc aaaataatct atactaagtt
     7081 attatctcta caactacaat ctgtatcatt acagtatttt ggcaacaata ttatcttgat
     7141 tactgacaca tattcaaaaa ccttagaatt attagatgaa gaattattaa aacaatattt
     7201 gcatataaag catctttcta gtgataattt tgattctgat atattagaga attgcaaact
     7261 tgaaagttat aaaataaata agcatttaat atcaaataga atttttagat taataaaaaa
     7321 tgaaatagat tgtatatcat taaaaaacct ttaaaggaga tataagctat tatgaaaaaa
     7381 aataaaatca gctttagaaa atggttcaaa ttttatctta ttggatgctc atgtatttgt
     7441 ataattgtaa gtttatttat gttaatatat tttggttcaa atagaattga aacaatggaa
     7501 actcattcag catataattt tatagaaagt aaaattccaa ccaatgctaa atatcaagga
     7561 tataaaaaaa atcatatcaa tgctaaaaca gtactttatt atagttataa agattcaata
     7621 catacagtag agttatacca tccagaaaat aatcttaatg aagtagattg gaatgaggtt
     7681 actgatatta aatttgatta atataaaatg aaaatattat atgataaaag cctagaaaaa
     7741 caattctagg ttttttattg tattctttat taaacgctac cgttttattt ggattttaac
     7801 gcctccataa tcatcaccct ttgtgattat acatccagca atataaacag cttacaagca
     7861 tctttaaatg ttttatttta gattaagaaa ttattttaga ttaaaggata aaattctaac
     7921 taatataagt gcttttaagc tattttttat aacataaaaa aattatattg gaaaccttat
     7981 aaaaaatatt cgtaaataaa caaaaaagct atataaataa ttaatattta aaatagcttt
     8041 tatttatact gactaataca ttttttaatt ttcatcaaat ataatgttat agtcttctgg
     8101 tttacttttt tctatttcta aacttccttc ataggcttct ttagaatagt catttggtat
     8161 atattcttca atgattcttg tatttctaaa cttaatcatt cctacacctg ttccaaattc
     8221 ttcattacaa taactgtatt ctatttctac agtgggaaat ataagagaaa gtttaattat
     8281 tatttgagtc acattagacc atgcagtaaa aaaataaata gtatcatccg tacttctttt
     8341 atctatacca ccaatagcat tccatttagt accccaattt ttcatacacc attcaagcca
     8401 acaattttcg tctctatgtt ttttacattc atttttactc aaactacctt tataaatcca
     8461 acttggcatt gaaattattt tattaaaatc aatactaccc acacctattt catcattttt
     8521 tataaattct aatatttctt taatttcttt ttcttcccct aaaaaacata atctatttgt
     8581 tattttatct gacatatatt cctccatact attttaattt tatatttata agtattatat
     8641 atatgttata atttttataa gtttttttac ttattgtgtt ggttacttct accatcactt
     8701 ttcttattat ctaagacttc taagaactta atcttgatgt attctctcaa aatatcctca
     8761 actttaacaa ctccaaaaga tgtcgctaga ccattccaat cctttacagc atacataaat
     8821 tttatttctg tctcattaat attagttatt aaaacatctc ttgaaaattt ataccatcct
     8881 gctatatcaa agtattcaag tcgtatagct ttataaatat taaatttgtt tgtatcaaat
     8941 ttattatgta ttatgtattc cacactaaca cccctttata taatttaaat tttatatagc
     9001 atttgaataa ctaattccac aaagttctgg taaattagat tcaactaaaa ctttagataa
     9061 tattggagga actgagttcc cacatctagc aacttgttca gttttaggat ataaattcca
     9121 tttataatct ctatctatta cataactttc aggaaagcct tgtcctctaa ataattctct
     9181 tggtgtcaac attcttaaac ctatatcatt tattttttgt aattgcctat taagactttg
     9241 acctatctca cttccataat actttaaaaa ttcatataac tcttcattat ctggtaatct
     9301 aatatcatta aaatcaactc gtacaagtcc aaaacgatta gccgtaggta tagtatttat
     9361 tggttttctt aagctactac ctctagtttc taagccttga tgtgtataat aatgagttag
     9421 aaaataagca cttttattta ctatatatgg tttatcttct atgataaatt tatttattcc
     9481 tcttgctatt cttaccaaag tattttcact taaaggtttt tttctattga atatacttgg
     9541 aacttctata tcccaatcaa ttatttcaga tgcaggtata taaggaatct tattttctcc
     9601 atgtgttggt tcggggaata ctataggttt attatcacac cttgcaatta aaaaaaatct
     9661 ttttcgtgtg gtaggtgctc catagtcaca tgcttttaat tccttatatt caactttgta
     9721 tcctaaacgt tccagagcat ttataaactt atgaaaagtc tctccttttt ttgatttaac
     9781 tggtctatca ttttttacag gacaccatgt tttaaattct ggtacatttt ctaatataat
     9841 tactcttggt tttactttag atgcccaacg aacagctacc catgctaatc ctcttatttt
     9901 tttatctaca ggtgttttcc cttttgctcg gctgaaatgt ttacaatctg gactaaacca
     9961 acataaggct actggttgac cttgtgttgc ttctactggg tctacatccc acacattttc
    10021 gcaataatgt tttgtattag gatgattagt cttatgaagt cttattgatg atgggtcatg
    10081 atttatagct atatcaactt ttcttttagt tgctaattca attccttcac ttgctccacc
    10141 accacctgca aagttgtcaa ttattaattc tttcatatac actactcctt tcatataagt
    10201 caaagtaagt ctaaatagat ttaataggta attttataaa tttaaaatat ctctatacca
    10261 tataacttta ttgtagattt tagcaaattt tctttattct catttattaa ttcaaagtcc
    10321 acttttagaa atagatttat ctcttctaaa tcataaatat gacttttata ttcagtaata
    10381 tcttcttcat aaaattcaga ccattttttg aaattaccaa tgtattcacg atataatttt
    10441 tttactttac cattaaattt gcatatttgt aatttcttat tattaatctc tttaattgat
    10501 tttatgaaca ttgctatagt tgtatcatat ttttcctttt tatttatcat ttcttgaatt
    10561 ttatcaaata ccttttcatc aatatctttt aatgtgaatt ttttttgatt atagattctg
    10621 attatatcat cataatcata taaataatca tattcaccat tatatatatt ttcatcaaga
    10681 gtttgacctt cacaatgtat ataatggtga attttatctt caattatata aaatccacaa
    10741 cactttaggt tttctataga agttacttct atagtttctc taatacactt tttgacttta
    10801 gatttagcaa tattcatttc aacccctcca aattttaaag tatatttcta tttctttcta
    10861 tatttatatt ataacatata atacactttt tagcaatctt tttattgtat agattcatat
    10921 aaatcaagca cttattctta aataaaaaaa ttgtgttcat agaatataaa atctacaaac
    10981 acaatctatc tataatacac aggttatttt ttaacacatc attactattc atttttttag
    11041 gattattttc tgcctttttt attttctctt ctctagtttc caatgtaatt tatcccctct
    11101 ttatttttat ataagattct tatagcttta tatgttagtt ctttttcttc acgatttaat
    11161 tttgcaaaca tatttatata tccattaaaa actatatttt cattaagacc tacatcatca
    11221 tatactaaat agtcaagtga gcattgaaaa taatcagcta atttttttaa tgttgagagt
    11281 tttacattat cagttccctt tacatagaat ccagaaatag ttgtgtaagg aataccactt
    11341 tcctttgaaa gttgagactt ggttatattt ttagagttta ataattcgtc tatcttttct
    11401 gtcatactca attttaaaca ccacctatct taatatatag atatttaatt atttaatact
    11461 tttttcatta ttccaataat caaatctttt gtttcattat caatttgagc gaataaataa
    11521 ttaaattctt ttgcttttat atctatattt atagtgttca tatcatcaaa atcatttagt
    11581 gtgcaattta atgctctagc aagtgattta agcgtttcaa atttggggtc ttttgttgtt
    11641 ccatttagta ttttatctaa tgttccttta ggaacatttg acaactcaga taattgctct
    11701 gatgtgagat ttctattttt tcttaattca tttattttct tgccacaatt gctttctttt
    11761 gaaaaaattt ctcctgtacc agtttctagc cactctttat taacattata tatatcacaa
    11821 atatctgatt taacacgttc agtcaatcct actttgccag tctcaatatt tccataattt
    11881 gagcgactta tttttaatga tgcagacatt tgttcttgtg taagttttaa aatctttctt
    11941 aattctatta aatttaatct tataattcca tctttttcag acattaacct cacctacttt
    12001 ttaatgattg ttgcaatttt attaattctt taatttgatt aatcgcacat tctcaacttg
    12061 ttttattttt tcattttcta ttaagatttt aattctcttg cctatatcgc acattaatat
    12121 cacctctgta ttgacctttt tacaatctca ttcaaagacg ctatgtatgt agcatctaat
    12181 ttagaaatgt taattattag ctcttttaaa ttttcatcct ttgaactagc tatcttatag
    12241 aatacttcgc ctaaagttga ataaatatca tcttctacag aaacatacat atcacctgta
    12301 ccatttataa gccattcctt attaatatta aatacactac ataaatgatt aataagtaaa
    12361 ggtttgggtt caaccctatc atactctata ttacttataa catctttact gacgcttagt
    12421 ttttccccaa aagctggttg agataaatta gcatctttcc ttatctttct aattcttgtg
    12481 cttatagttt catcagacaa ttataatacc tcccactatt taaattagac cttttacaat
    12541 ctcatttaaa ttatttatat atgtatcatt taatttagaa atatttataa tcaattcttt
    12601 taaattttca tttttagata cagttatatt agcaaaaact tctcctaaaa caatatcaca
    12661 ttcttgttca ataaacttcc tcccctcacc agtttctaac cacacttgat taacactaaa
    12721 aacctcacac atatgagtaa taaatactgt tttaacttct actctattat tttctatatt
    12781 acttataaca tctctactca caccaagctt ttctccgaat ttttgttgtg ataaatttat
    12841 atcttccctt atttctttta ttcttttatt tgtatctata tcaatcataa tacgctcctt
    12901 aaattttaat agcatctttt atatctctta ttgctctatc tatatttata ttatttatat
    12961 atctaagttt attattttcg tttagatttt ctataatttt gtaatacatt gcatgtccta
    13021 cataattagt atttataaaa acatactcat aactattcaa tatatttaca tcaaaattaa
    13081 ttgtttctgc tgatataaat tcaaagttaa ttaatttatc tttcattttt aaaatccaat
    13141 ttggatgtcc tccaaaaatt atcgctttta tgtttttcaa atctctttca tcagtgggaa
    13201 tattttcaat atattcctgt tgtttgctaa acataaaatt tcttaattga accaattctt
    13261 ctttggatgt aatttgtgct ttgatttcat tttctaatct aagtttgtct ttttgcaata
    13321 attccaattc taattttatg tttttatttt cttctcttaa taaagaattt tcttttttta
    13381 aagtaattaa ttcaatatct ttttcttcta tagattcttt taattcttca tcaaaattat
    13441 taaagaaaaa ttgctttgct tttttatatt ctacaagtag atacctcaaa tctatagcag
    13501 gaattataaa tctcaataaa tcttcataat taatactatt ttcatcctct atattatttt
    13561 gtaaactaca ataacataaa attaattctt gtatttgttt agatttaaat atatcttttc
    13621 ctacaattga aattacactt attgcttcta aatcatatac atagtcaaag cataaaaggt
    13681 ttctgtctaa ataggataaa tcatctatct taaaagttcc tgtactctca ttgttgactc
    13741 taattgcata agatgatggt gtatattctt ttaaatatat agattttaat ttcaactcta
    13801 ttttgctaat aagtttttgt tcttctttac ttagtttatc tatagaaaaa aatttttcac
    13861 atttatagct ttcatttctt aaaatcaaat ttttgattac ttttacatat atctcatctg
    13921 attcatcaac ttctttttct aaagctccac ataaagaaat agcaactaac atatttccat
    13981 taagttcatc atcagagaat ttttccattt tattttttaa cataagtttt cttaaaaaag
    14041 tacttgcttt aaatattggt acagaaataa tgtaattata agcgattgta taacctacct
    14101 taaatattct agttattttc ttttcatctt tttccaataa tatacctaga gattttttaa
    14161 aataatattc ttggattata cttccttcac ttgctattct tgaatcataa aagatatttt
    14221 ctttagctaa atttgaatat tttaatttat tttcattata atatttatct atgagtttat
    14281 aacatttttt atttgtcgat aaagtctcag ctaaaaatgg tattatattt aattcttctt
    14341 gcatctgaaa acctcacttt tttaatgaaa ttaaaagata taaattcata ataccttata
    14401 tctttcaatt tcacattgta atattagtta atatctaatt attatattat aacaatataa
    14461 tctctcttat ttttatttta ctttcacaaa tttcttcttc attcctttct attacttcaa
    14521 aaccaatatt taaattaaca gtcctattta aatcttcata aaagaaaata tatgtataac
    14581 ttcctgactc caaataatca tttatatctc cttctgtgaa ataatcccat gagtctaatt
    14641 tatcactaag atgattatgc ttaccttgta caaaattatc atattcaaat attgtaaact
    14701 cttcacctat agaatccttt atttcttgta taaaatcttc tatatcttct attcgttcat
    14761 tataattata aatcatgttg tttacatttt cataaataat agtatctata tcttctagat
    14821 tatattcttt tcttctatat tcttctataa tatcctcaat ataaattatt tcttcataat
    14881 cattattata tatattttca tccatgccct gaccaacata agatataaaa aattgtactt
    14941 tatcattttt tatgtaaaat ccacatctgc ttaatttcca attaaaatta ttacttgtag
    15001 cttctctcac atatttttta atttctttct tagttattct tttcattttc atatcctcct
    15061 tatccacttc tttctatatt tatattataa catacaatac acttttttgc aatcttttta
    15121 ttgtattaat ttatttttca aaggctatgt caataacttt ttatgtttat gtaataacac
    15181 ttagatatat tgaaattaaa aggtatagga ttataaaaga ttattactga atcaaataaa
    15241 ctactaaatt tataataata aaaatggcga acattatatt agttcaccat tttattattt
    15301 aagttttttg tcattcataa aaagatataa tattctcaat cttatctagt acaatgttca
    15361 tattttttat aaagttttca gataaaacat atttttcatc tacataacaa ttttcatatg
    15421 atattatatc aaagtttagt atttgtatag actctaccaa atacattttg aactcttcat
    15481 tattctatta taatatctat ctaacatatc ttttaaaagt acttttttat atcatttaaa
    15541 gctctagttg catccatttt ttaaatactc cttttatatt tgtgtttcta tagtattatt
    15601 ataacgctct aaattaatat tcactttttt ctcaattaaa atttttctaa aaattcaaga
    15661 taaatttgtc tatataaatt tactctatga tgaatattat aaccaccttc ttcattgtat
    15721 attttataaa taaatctccc tttactcata tctttccacc catcatctaa gtaaaataaa
    15781 ttacaaagag taaataacca ttttccattc tctttaatgg caattatatt ttgaatttct
    15841 tcttctgctt ctttaaattc aatattgcta aatgcgaact tatacttatt atctcttttt
    15901 aactgttcta aactatttaa gaatttaata tcatcagaaa acatcttcaa atatttttct
    15961 ttatcatcta tatataaaat tgaatgttta tcttgtttta atgcttctat acatatttct
    16021 tcagtacgct tatccacaaa ttttaaagcc attccatttc gttttactgc ttctaagcac
    16081 atttcctctg tctgttctct tacatatttt aaagttaaag gttcttttct aatggctatt
    16141 agacaaagtt ctcttgtttg ttcttttaca cttgttaatg catatgcttt attatttata
    16201 gctattgaac aaagttcttt tgtttgttct tttacataaa ataaagacca accactttgt
    16261 tttactgctt ctttacatat ttcataattt tgttttataa catgcttcaa cgctccacca
    16321 tattgtttta ctgctaataa acatatttct tctgtttgct tttttacata ctccaacgct
    16381 ccaccctctt gttttactgc ttctttacat atttcttctg tctgttcttt tacatcttta
    16441 agagcaaatc cattttgttt tactgctaat aaacatattt cttctgtctg ctcttctaca
    16501 taaaataaaa ctcttccatt ttgtcttact gcttctttac atatagcttc tgtctgtttc
    16561 tttacatact gtaaagcttt accattttgt tttactgctt ctatacatat ggcttctgtc
    16621 tgttcatcca caatttccaa atctaaacca ttttgtctaa ccaattctat gtaaatggct
    16681 tctgtacgtt cgtccaccag ttcgaaagct tttctatttt gttttattgc ttctatacat
    16741 acagcttctg tctgtttctt tacatactgt aaagctttat aattttgttt tactgcttct
    16801 atacatattt cttctgtctg tttctttaca tactgtaaag cttcataact ttgtttcact
    16861 gcttctttac acacaacttc tgtttgcttt tttacatact tcaacgcatc accattttgt
    16921 tttactgcta atgaacagat ttcttctgtc tcttctttta cagctttaag agcaagtcca
    16981 tttcgtttca ctgcttcttt acatatttct tctgtttgat tgtgtacata cttcaacgct
    17041 ctaccatttt gttttactgc taataaacat atttcttctg tctgttcttt tacatcttta
    17101 agtgcaagtc cattttgttt cactgcttct ttacatattt cttctgtttg attgtgtaca
    17161 aattccaacg tccatcctat atatctcact gctagtaaac acatgtcttc tgtttgcacc
    17221 ttaacatatt ttaaaaaata agggtctttt ttaatttctt ttaatatttc ttctttttta
    17281 tccatactat atttctcctt tttcatgatt tcatgagatt ataaaaaacg ctaaacaata
    17341 tctatttttc cttttctaac ttctataata ttattatatc acaatataca cttttttgca
    17401 atctttttat tgtattgaat tgcaatactt ttatactaaa aagtcttttc
//
LOCUS       DAYASV010000083        17319 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYASV010000083 DAYASV010000000
VERSION     DAYASV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.083"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            74..2143
                     /locus_tag="ACGI68_002543"
     CDS             74..2143
                     /locus_tag="ACGI68_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0138976.1"
                     /translation="MQLTKIILSNYRCFGEEPTTIEINNLTGFIGHNSSGKTALLSAL
                     MKLFGEKSSDRIIERSDFHIPESIDPDSISKNELYIEAQFEFPELERGKDSISIPVFF
                     KNMVVSEPGGKPYLRIRLEANWEKSSNPEGSIDSNVYYITSTTEEINPEDKRNIKRYE
                     LDQIKMIYVPAIRRPDEQLKNVSGTIISRLLNGISWSEQTKARIKTKASETEEIFFEE
                     KGVESLKKSLETQWNSYHKDIRYSNPKINFNSTDLETILKKVEVTFSPTGTHRDYRVE
                     ELGDGLRSLFYLSLVNTFLEIEEEILKSINDGKEEDIFTLIPPVLTIVAVEEPENHIS
                     PHLLGRVVEKLNFISTKENSQTILTSHSPAIVKRIEPTDIRYFRICKEYECTKVKSII
                     LPNEEKEAEQFKFVKESVLAYPELYFAKLVVLGEGDSEEIIIKQMIELKAESVDSSEI
                     SIVPLGGRHVNHFWRLLNDLEIPFITLLDLDRERDGGGWGRIKYAIQQLINIGHDKNV
                     LLYTCEGISQDNEFKEMHTWDVTDTENMAKWIEMLKGYDVYFSAPLDIDFAMLQSFKD
                     KYISALTSAEGPRIKDHGKIQDIVINEDAEDESLKAAYKDRIDSDIKSTLKQEGGDGS
                     TYTEGEKELMIWYSYFFLGRGKPTTHLSLFSNNTDIDFESFPYCLKEFVNSIIEKMID
                     DSGGGRK"
     gene            2140..4005
                     /locus_tag="ACGI68_002544"
     CDS             2140..4005
                     /locus_tag="ACGI68_002544"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFH0138977.1"
                     /translation="MKRNWVSKEDWSPAAGFRLEVEAMNTVINDKNTLVVAGPGAGKT
                     ELLAQRACFLLETNTCRYPKKILAISFKKDAAENLKERLELRCGKELASRFESKTYDA
                     FAKEIVDRFRNSLDEAYRPLNNYNIAENRDVRRAFEIAGLNFRGNQTEFNKVYGNKLS
                     INKLPLHTDFVDKIFIDAWNVLLKGNKDTNFVSSLTFGMISRLAEYLIRTNRYIKKAI
                     EMTYSHVFLDEFQDTKSIQYDLVKACFFKTETIITAVGDGKQRIMLWAGARKSIFEDF
                     QNDFLSQKRTLIMNHRSAPRLVEIQKAMYNSLNEEAIDLKTNEKWNYEDGEVYLRLFE
                     DYIKEADILSEEIVELVETGIEINEICILVKQSVDIYSKEIINKLSERNIKARNEAMY
                     QEILKEDIIKTLVSLFRLSLSDRNPDDWDYIYDILGELYGIDEGYNPKILNDFITKVE
                     LMINDLKVNLNITDEKQFSDLVDSKVEYISYEKFASKFQKYKSKDYFDDLVDKFKKLV
                     WIEYNETKDWIKAIDNFEGKNSIPIMTIHKSKGLEYDTIFFVGLEDSAFWNFKNQPDE
                     DRCAFFVALSRAKRRIDFTFSLDRPAGFSNSQKHYCINEFYELFDNTEIVKKIEY"
     gene            4129..4323
                     /locus_tag="ACGI68_002545"
     CDS             4129..4323
                     /locus_tag="ACGI68_002545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138978.1"
                     /translation="MKKKTKISKKIVSFIMVFITIVTTILTSSVSAYASELDMSEQTG
                     FNYLGISPITGKKLIITFIV"
     gene            4326..4943
                     /locus_tag="ACGI68_002546"
     CDS             4326..4943
                     /locus_tag="ACGI68_002546"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138979.1"
                     /translation="MDNKIVFCVEPGIKTSSGGGYISEELGDKFISTTLPNYHKRKAE
                     ILEKVNRHNILPSWNNEKVAVKSGETIELEDKNGIIREMQLESNNTGTDILLEENTLK
                     ITANKNSNSGVISYIKIPKNEVGISIVYRKHNYQSLSEFHMESSMSANLNINVIKLGI
                     VKVKKIDEGTGKELANAKLRFEYDGDSKEIITGSNEIASIEDIIG"
     gene            4935..5456
                     /locus_tag="ACGI68_002547"
                     /pseudo
     CDS             4935..5456
                     /locus_tag="ACGI68_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901831.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            5569..5730
                     /locus_tag="ACGI68_002548"
     CDS             5569..5730
                     /locus_tag="ACGI68_002548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138980.1"
                     /translation="MAIAIIGVYLGFKREFSKLIGFLVVALIAVGLVFNTAGVKDILL
                     ELFNKIIGV"
     gene            5970..6197
                     /locus_tag="ACGI68_002549"
     CDS             5970..6197
                     /locus_tag="ACGI68_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0138981.1"
                     /translation="MDIVKVFANNVRKYRNKKGLSQEAFAEKAGLHRTYICAIERKKR
                     SIALDNVQKIADALEIETYLLFIEGEEKECL"
     gene            6194..9133
                     /locus_tag="ACGI68_002550"
     CDS             6194..9133
                     /locus_tag="ACGI68_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0138982.1"
                     /translation="MKKLPIEKLIENLGFSGSKNLVNIDMLTKLDISKHDVKTLKVIN
                     PYAIYLVNSKPFILFIESSFEEKKLQQISKLIWNAQIPIAFICDSNSVKIFNGKALDL
                     KSYLIEEIYNQSIEDFSEESEFSYLKISDPLFWDQYTKQYTKTHLNDYLLDNITSLTN
                     KLKKKYKVKFATKLVLRLIFIRYLIDRGVDIAYKNFNHDIKASQKELLKITKNKVELY
                     ELFKYLKEKFNGNLFELNDEINNTCLTDEVFILLSDFLSGKISIDNGQMSLFSMYDFN
                     IIPVELISNIYEILLGKEIRNKDNAFYTPNYLAEYILDKTTLEFLKNNRQYKILDPSC
                     GSGVFLVNSFRRIIDMNLGDKLFCEDDKLLKELLLSNIYGIDINEDAIDVTIFSLYLT
                     ILDYKDPKILSEFKLPNLKGCNLIASDFFDDNKLESLKKINFDFIIGNPPWGSVSDGL
                     HLDYCNKNGYNDMQQNKEISRSFIFRAKDFSSRNTVCSFVVHSKILYTQKKPSKKFRE
                     FLLKKSQIFSVVEMSSVRKLVFKNADAPAAVISFKYNESEENLNNRIIYTSLKPNVFF
                     KLFNIIVIEKYDVKYISQKLLLENDWAWKTIVYGFSKDIDIIMNIKKKYQTVNEIILK
                     NSFVHGTGIQINGNESHDATHLKGKWYVDSKKGIDSFYVDINKGKELTQDKMRRPKAN
                     QQEIFIPPYVLLKKGVDTKTYRYRAAFSNDAFVYSDAVTGICGKKDNLTTLYCLTGML
                     NSSFYSYLNIMLGTSIGIEREQGFSTEIFTYPAIESDEIANLVVKIQEATKLEKTTMF
                     LNEKNSCKYIKQLDKLILKSFNLNDDKFIDYAINIQIPELTNSKSTNIYRKITKEDLI
                     NYSECFKEQFSSIYRRVGKSISISVYPKILNRFTVFELEVTDEKTDREIMVKAEADNN
                     MELFTKIIRYDYNDVFHQIRDIIHFGENSFYIIKPNCYKYWHPAIAEMDLADVLDQIL
                     SDTGGEE"
     gene            9133..9807
                     /locus_tag="ACGI68_002551"
     CDS             9133..9807
                     /locus_tag="ACGI68_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138983.1"
                     /translation="MGGFKNSSLNFYLKQGLYEIIIDGLIDCCLLMSKSCIEQSRKIL
                     NHEEKIRTHLVENYLENDIERRKTSLSKQHLRFEVEAQENYDNKNESYEGRVDIKVIS
                     KDTLADRRAYYIIECKRIDGKETLNKKYITEGVQRFITEKYSSHYGKNIMLGFIVKDI
                     NIQSNIQKINSLNQNILYTYIDTDMLTVRSDSENNYAVCESKYKVKSKLLLLNHIFYN
                     FSSIIR"
     gene            9968..10384
                     /locus_tag="ACGI68_002552"
                     /pseudo
     CDS             9968..10384
                     /locus_tag="ACGI68_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003455547.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            10344..12791
                     /locus_tag="ACGI68_002553"
                     /pseudo
     CDS             10344..12791
                     /locus_tag="ACGI68_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            12931..13353
                     /locus_tag="ACGI68_002554"
     CDS             12931..13353
                     /locus_tag="ACGI68_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138984.1"
                     /translation="MRLEDIIDKESYEEKYDDLTVEIKKLSKEKKTLKASLINEKSIK
                     ARLREFRKILERNELLEKFDRYVFASIVDKVIVGVINEDGIVDPYKITFVYKTSFDDK
                     QDGHKFINKSPLETYSKDNNDNKKKCSYTNNETLRDCS"
     gene            13891..14925
                     /locus_tag="ACGI68_002555"
     CDS             13891..14925
                     /locus_tag="ACGI68_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HFH0138985.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTTTSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKVAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSEFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            15012..15215
                     /locus_tag="ACGI68_002556"
     CDS             15012..15215
                     /locus_tag="ACGI68_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFH0138986.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            15386..16546
                     /locus_tag="ACGI68_002557"
     CDS             15386..16546
                     /locus_tag="ACGI68_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HFH0138987.1"
                     /translation="MKLSKILLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGRVSTVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSMSDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            16805..16945
                     /locus_tag="ACGI68_002558"
     CDS             16805..16945
                     /locus_tag="ACGI68_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138988.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
BASE COUNT         7091 a         1681 c         3003 g         5544 t
ORIGIN      
        1 tgtataataa aggaaaaaat acagaattaa aaaacataag atagataagt ggacttaggg
       61 gagagaaatt attatgcaat tgacaaaaat aattcttagt aactataggt gttttggaga
      121 agaacctaca actattgaaa ttaataattt aactggattt ataggtcata atagcagtgg
      181 taaaactgct ttattaagtg cacttatgaa actttttgga gaaaagtcta gtgatagaat
      241 tattgaaaga tcagatttcc atatacctga aagtattgat ccagatagta tttctaagaa
      301 tgaattatat attgaagcac aatttgagtt tcctgaattg gagagaggta aagacagcat
      361 atctatacca gtatttttta aaaatatggt tgttagtgaa ccaggtggga aaccttattt
      421 aagaattaga cttgaagcaa attgggagaa aagctcaaat cctgaaggaa gtattgattc
      481 aaatgtatac tatataacat ctacaacaga ggagataaat cctgaagata agcgtaatat
      541 caaaagatat gagcttgatc aaataaagat gatttatgta cctgccatta ggagaccaga
      601 tgaacagtta aaaaatgtct caggaacaat aatttcaaga ttattaaatg gtataagttg
      661 gtcagaacaa acaaaggcaa gaattaaaac gaaagcaagt gaaacggagg aaattttctt
      721 tgaagaaaaa ggggttgaat cattaaagaa atcactcgaa actcaatgga atagttatca
      781 taaggatatt agatattcta atccaaagat aaacttcaat agtactgatt tagagacaat
      841 tttaaaaaaa gttgaagtaa ctttttcacc tacagggaca catcgagatt atcgggttga
      901 agagcttgga gatgggttac gttctttatt ttatctttca cttgtaaata catttttaga
      961 aattgaagaa gaaattctaa aatctattaa tgatggtaaa gaagaagata tatttacatt
     1021 aataccgcct gtattaacaa ttgttgcggt tgaagaacca gaaaatcata tttcacctca
     1081 tcttttagga agggtagttg aaaaattaaa ttttatttct acaaaagaaa attcacagac
     1141 aatattgaca tcacattcac cagcaatagt aaaaagaatt gaaccaacag acataaggta
     1201 ttttaggatt tgtaaggaat atgaatgtac aaaagtgaag tcaattatat taccaaatga
     1261 agaaaaggaa gcagaacaat ttaaatttgt aaaggaatca gtattagcat atcctgaact
     1321 ttattttgca aaacttgttg tattgggcga aggtgatagt gaagagatca ttattaagca
     1381 gatgattgaa ttaaaagcag aatctgtaga ttcatcagaa atttcaatag taccattagg
     1441 tggtaggcat gttaatcatt tttggcgttt attaaatgat ttagaaattc catttattac
     1501 tttgttagat ttagatagag aacgagatgg aggtggttgg ggacgtataa agtatgcaat
     1561 acaacaatta ataaatattg gtcatgataa gaatgtatta ctttatacat gtgaaggaat
     1621 ttcacaagat aatgaattta aagagatgca tacttgggat gtaactgata ctgaaaatat
     1681 ggctaaatgg attgaaatgc taaaaggtta tgatgtttat ttttcagctc cgttagatat
     1741 agattttgca atgttacaaa gttttaaaga taaatatatt tcagcattaa caagtgcgga
     1801 aggtcctagg ataaaagatc atggaaaaat acaagatatt gtgatcaatg aagatgctga
     1861 agatgaatca ttgaaagctg cttataaaga tagaatagat agtgatatta aatcaacctt
     1921 aaaacaagaa ggtggtgatg gatctactta tactgaggga gagaaagaac taatgatatg
     1981 gtattcatat ttctttttag gaagaggtaa accaactact catttatctt tattttctaa
     2041 taatactgat atagattttg aaagttttcc atactgtcta aaggagtttg taaatagtat
     2101 tattgaaaag atgatagatg attcaggggg tggaagaaaa tgaagaggaa ttgggttagt
     2161 aaagaagatt ggagtccagc ggctggattc agactagaag ttgaagcgat gaatacagtt
     2221 attaatgata agaatacact tgttgtagct ggtcctggcg ctggaaaaac agagttatta
     2281 gctcagagag cctgcttttt actagagact aatacctgta gatatcctaa aaaaatcctt
     2341 gctattagtt ttaaaaagga tgctgctgaa aatttaaaag agcgtttaga attgagatgt
     2401 gggaaagaat tagcttcaag atttgaatca aagacctatg atgcttttgc aaaagaaatc
     2461 gtagatagat ttagaaatag tttagatgaa gcatatagac ctttaaataa ttacaatata
     2521 gctgaaaatc gtgatgttag aagagcattt gagatagcag gattaaattt cagaggaaat
     2581 caaactgaat ttaataaagt atatggaaat aaactatcga taaataaatt gcctttacat
     2641 acagattttg tagataagat ttttatagat gcttggaatg tactacttaa aggcaacaaa
     2701 gatactaatt ttgtaagttc acttactttt ggtatgatat cacgtttagc cgaatatttg
     2761 ataagaacca ataggtatat taagaaagca attgaaatga cttatagtca tgtgttttta
     2821 gatgagtttc aagatactaa atctattcaa tatgatcttg ttaaagcttg tttttttaaa
     2881 acagaaacaa taattactgc tgtaggggat ggcaaacaac gaattatgtt atgggctggt
     2941 gctagaaagt caatatttga agattttcaa aatgattttt taagtcaaaa aagaacatta
     3001 attatgaatc atcgttcagc gccaagactt gtagagattc aaaaagcaat gtataacagt
     3061 ttgaatgaag aggcaattga tttaaaaact aatgaaaagt ggaattatga agatggagaa
     3121 gtgtatttaa gactttttga ggattatata aaagaagctg atatacttag tgaggaaata
     3181 gtcgaattgg tggaaacagg tatagaaatt aatgaaatat gtatattagt aaaacagagc
     3241 gtagatattt attcaaaaga gattataaat aaactttctg aacgtaatat taaggcgcgt
     3301 aatgaagcaa tgtatcagga gatattaaaa gaagatataa taaagacact ggtttcgctt
     3361 tttaggttgt cgttaagtga tagaaatccc gatgattggg attatattta cgatattcta
     3421 ggtgaattat atggaataga cgaagggtat aaccctaaaa tattaaatga tttcattacc
     3481 aaagtagaat taatgattaa tgatctcaag gtaaatttaa atattactga tgaaaaacaa
     3541 ttttcagatt tagttgattc taaggttgaa tatatttcat atgaaaaatt tgcatcaaag
     3601 tttcaaaaat ataaatctaa agattatttt gatgatttgg tagataaatt taaaaagctg
     3661 gtatggattg aatataatga aactaaagat tggattaaag cgattgataa ctttgaaggt
     3721 aaaaattcaa ttccaataat gacaattcat aaaagtaaag gacttgaata tgatactatc
     3781 ttttttgttg ggttagaaga ttcagcattt tggaatttta aaaatcaacc agatgaagat
     3841 agatgtgcat tttttgttgc attatcaaga gctaaaagaa gaattgattt tacatttagc
     3901 ttagatagac cagcaggatt ttcgaattca cagaagcatt attgtattaa tgaattctac
     3961 gaattgtttg ataatactga gattgtaaaa aagatagagt attgattaag atgataaaaa
     4021 ctttagtata ttatatttta agatattagt tatataagta gacattgaat ttatgtattt
     4081 gatgtctatt ttttatataa aaatatatta aagaaaggat gaatttgcat gaagaaaaaa
     4141 acaaaaatat ctaaaaagat tgtaagtttt attatggttt ttataactat agttactact
     4201 atacttacat catcagtttc agcttatgct agcgagttgg atatgagtga acagacaggc
     4261 tttaattatt taggtattag tccaattaca ggaaaaaaat taatcataac atttatcgta
     4321 tgaagatgga taataaaatt gtattttgtg tagagccagg aattaaaact tcaagtggag
     4381 gaggatatat atcggaggag ttaggagata aatttatatc tacaacactt ccaaattatc
     4441 ataaaagaaa agctgaaata cttgagaagg taaatagaca taatatatta ccttcatgga
     4501 ataatgaaaa agttgcagtt aaaagtggag aaacaattga gttagaagat aagaatggta
     4561 ttattagaga aatgcaatta gaatcaaata atactggaac agatatatta cttgaagaaa
     4621 atactttgaa aattacagca aataaaaatt ctaattcagg agttattagt tatataaaga
     4681 ttcctaaaaa tgaggttgga atatcaatag tttatcgtaa gcataactat caaagcttat
     4741 cagaatttca tatggaaagt tcgatgagtg caaatttaaa tataaatgta atcaagttag
     4801 gaattgttaa agtaaaaaag attgatgaag gcacaggaaa ggaattagca aatgcaaaac
     4861 ttagatttga gtatgatggt gatagtaaag aaattataac gggttctaat gaaattgcta
     4921 gtatagaaga tattattggt tgattaaaaa gtgaagtata cttttgtatc tatgaaaaag
     4981 actataagca atatataaaa ttagatatac ctattgatga agcaaaaata aaaaatagat
     5041 ttgaaataag gttaaaaaat gaaagagcat atcatgcagc agttgatttg ttagcaaata
     5101 gagatgaaga aaagacagca ttttcaatta ttaatcgtta tatacgtttt gttgataaag
     5161 atgatgaaaa aattagaagt gaatggaaaa ctaatgatca gtggagttat tttattggtg
     5221 aaggtagagc gaagttaaaa ctaacaacaa atccagaacc atataatatt caaaaaactt
     5281 taaattggtt atcaagacaa gttgcaccaa cgttaaaaac aattaaaagg ttagatagtt
     5341 tgaacaactc aacattaatt gagggcttaa taaggtatgc aagactaaca gataaacata
     5401 aaaaaataat tgaacaaaat tcagtaaatg ttgaagacat tatttatgat gaataaaaaa
     5461 atgtaattag tattgcagat tttaatataa aagaagggag atatacgaat gaattttggt
     5521 caaaatttat ataattagtt tttatcaaat gcacaatcat tggtattaat ggctatagct
     5581 ataattggtg tatatttagg atttaagaga gagttttcaa aacttatagg atttttagta
     5641 gttgcattaa ttgcagtagg gcttgtgttt aatacagcag gagttaaaga tatattattg
     5701 gagctattta ataaaataat aggtgtgtaa ggattaaaag agaatagaaa attatgagta
     5761 tatgatgaag ggttctaata agttttatta aaatatatat tgaaagacaa gtagagctat
     5821 ttcgagttat tacatgtaat gctaatatat ataactgcat tttggaaatt aaatacataa
     5881 ttagaaatag caaattttat aggtgactta ttaatatgtt tacttattgt atactatttg
     5941 atatatttaa tatgaggtga ttcttgaaaa tggacatagt gaaagttttt gctaacaatg
     6001 tgagaaaata tagaaataaa aaaggtctat cacaagaagc ttttgctgaa aaagctggat
     6061 tgcatagaac ttatatttgt gctattgaaa gaaaaaagag aagtattgct ttagataatg
     6121 tgcagaaaat tgctgatgcg cttgagattg agacttactt actatttata gaaggtgagg
     6181 agaaagaatg tctatgaaaa aattaccaat agaaaagctt atagaaaatt taggtttttc
     6241 agggtcaaag aatcttgtga atatagatat gcttacaaaa ttagatatat ctaaacatga
     6301 tgtaaaaact ttaaaagtta taaatcctta tgcaatttat ttagtaaata gtaagccatt
     6361 tatattattt atagagtctt cttttgaaga aaaaaaatta caacaaatta gtaagcttat
     6421 atggaatgct caaataccaa ttgcttttat atgcgattct aattctgtta aaatatttaa
     6481 tggaaaagct ttggatttaa agtcatattt aattgaggaa atatataatc aatctattga
     6541 ggatttttcg gaagaatctg agttttctta tttgaaaata tctgatccat tattttggga
     6601 tcaatacact aaacaatata ccaaaacgca tttaaatgat tatttgctag ataatattac
     6661 ttcattgaca aataaattaa agaaaaaata taaggtgaaa tttgcaacga agcttgttct
     6721 aagattaata tttattagat atttaatcga tagaggtgtc gatatagctt ataaaaattt
     6781 taatcatgat attaaagctt cacaaaaaga actgctaaag attactaaaa acaaagttga
     6841 attatatgaa ctgtttaaat acttaaaaga aaagtttaat ggtaatttat ttgagttaaa
     6901 tgacgaaatt aataatactt gtttaacaga tgaagtgttt attttactat ctgatttttt
     6961 atcaggtaaa ataagtattg acaatggaca aatgtcttta ttttcaatgt atgactttaa
     7021 tatcatacca gttgaactaa ttagtaatat atacgaaatt cttttaggaa aagaaattcg
     7081 taataaagat aacgcattct atactccgaa ttacttagca gaatatattt tagataaaac
     7141 aacacttgaa tttctgaaaa ataataggca atacaaaatt ctagatcctt cttgtggatc
     7201 aggagtattt ttagtaaata gttttagacg tattattgat atgaatttgg gtgataaact
     7261 tttttgtgaa gatgataaac ttttaaaaga acttttatta tctaatattt atggaattga
     7321 tatcaatgaa gatgctatcg atgttactat tttctcgtta tatctaacta ttttagatta
     7381 caaagaccct aagatattat cagaatttaa actgcctaat ttaaaagggt gtaatttaat
     7441 agcgagtgat ttttttgatg ataataaatt ggagagttta aagaaaatta actttgattt
     7501 tatcattgga aatccgcctt ggggaagtgt ctcagatgga ttacatttag attattgcaa
     7561 taaaaatggc tataatgata tgcaacaaaa caaagaaatc agtagaagtt ttatttttag
     7621 agcaaaagat tttagctcta gaaatacagt atgttctttt gtagtgcatt ctaaaatact
     7681 atatactcaa aaaaagccat caaaaaaatt ccgtgaattt ttgcttaaaa aaagtcaaat
     7741 atttagtgta gttgaaatgt catctgtaag aaaattagtg tttaaaaatg cagacgctcc
     7801 tgcagcagtt atatcattta agtataacga aagtgaagaa aatttaaata atagaatcat
     7861 ctatacatct ttaaaaccaa atgtattttt taaattattt aacattattg taattgaaaa
     7921 atatgatgtt aaatatattt cacaaaaact attattggag aatgattggg cttggaaaac
     7981 tattgtgtat ggattcagta aagatataga tataattatg aatatcaaaa aaaaatatca
     8041 aactgtaaat gaaattattt tgaaaaacag ttttgttcat ggaacaggga tacaaatcaa
     8101 tggtaatgaa agccatgatg cgacacattt aaaaggaaag tggtatgttg attcaaagaa
     8161 aggaatagat tcattctatg ttgatataaa taaagggaaa gaacttactc aagataaaat
     8221 gagaagacct aaggctaatc agcaagaaat atttattcct ccatatgtat tattaaaaaa
     8281 gggagttgac acgaaaacat atagatatag agcagcattt tcaaatgatg catttgtata
     8341 ttcagatgca gttacaggaa tatgtggaaa aaaagataat ttaactacac tttattgctt
     8401 gactggtatg cttaactctt ctttttactc atacttaaat ataatgctag gtacatcaat
     8461 tggaatagaa agagaacaag gattttcaac ggaaattttt acgtatccag caatagaaag
     8521 tgatgaaatt gctaatctgg tagtaaaaat acaagaagcg actaaattag aaaaaacaac
     8581 tatgttcttg aatgaaaaaa atagttgtaa gtatatcaaa caattagata agttaatttt
     8641 gaagtctttt aatttaaatg atgataaatt tattgactat gcaataaata ttcaaattcc
     8701 tgagcttaca aattctaagt caactaacat atatagaaaa ataactaagg aagatttaat
     8761 taattatagt gaatgtttca aagaacagtt ttcatcgata tatcgtaggg ttgggaaaag
     8821 tatttcaatt tcggtatatc caaaaatatt aaatcgtttt acagtatttg aactagaagt
     8881 aacagatgaa aaaacagata gagagattat ggttaaagca gaagctgata ataatatgga
     8941 gttgtttacg aaaataatta gatatgatta taatgatgtt tttcatcaaa ttagagatat
     9001 tattcatttt ggagaaaatt cattttatat cataaagcct aattgctata aatattggca
     9061 tcctgctatc gcagaaatgg atcttgctga tgttttggat cagattttat ctgatacagg
     9121 gggtgaagaa taatgggagg atttaaaaat tcttcactaa acttttattt aaagcaaggg
     9181 ctttatgaaa taattataga tggattaata gactgttgtt tacttatgag caaatcatgt
     9241 attgaacaat ctagaaaaat attaaatcac gaagaaaaga taagaacgca tttagttgaa
     9301 aattatttag aaaatgatat tgaaagaaga aaaacatctt tatctaaaca acatctaaga
     9361 tttgaagtag aagcacaaga aaattatgac aacaaaaatg aatcttatga gggtagggtt
     9421 gatataaaag ttatatctaa agacacttta gcggatagaa gggcttatta tatcattgaa
     9481 tgtaaaagga tagatggtaa ggaaacttta aataaaaaat atataaccga aggtgtacaa
     9541 cgatttatta ctgagaaata ttcttcgcat tatggcaaaa atataatgct tggatttata
     9601 gtaaaagata taaatatcca gagcaatata caaaaaatta attcactgaa tcagaacata
     9661 ttatatacat atatagatac agatatgttg acagttagaa gtgattctga aaataattat
     9721 gctgtttgtg aaagtaaata taaagtaaaa tctaaactac tattgttaaa tcatattttt
     9781 tataactttt cgagtataat aagataatct tatgaatata aaatattact atgttaaaat
     9841 tatagtttgg ttttgataat attataaaaa taagtttaag aataaaatat aaaggaaaaa
     9901 aggggcgtag cactattttt tagtgtgaca gcccctttaa ttttaaaatc ggtaaggagg
     9961 agtgtatttg aaaaaaatta agtcatatac agggattttg aatgtggaga aggttatata
    10021 tgcaataaat gattttcagc ttccatttcc agttactttt acgcagatga catggtttgt
    10081 attaacactt tttataatta tattatttgg taagttacct ccattattta tgatagaagg
    10141 agcattttta aagtatcttg gcataccaat ttttataact tagtttatgt ctcaaaagac
    10201 ttttgatgga aagaaaccat atggattttt aaagtcaata atactatatt cattaagacc
    10261 taaaataaca tacgcaggaa aaaacattaa atttaaaaat aaaattattg atgaaaatat
    10321 aactatagta aggagtgaat tatatgttcc cgataaaata tatagagaac aatcttgtat
    10381 ttaataagga aggagaatgt tttgcatatt atgaattaat accatataat tattcattct
    10441 tataagctga acaaaaattt attgtacatg ataattttag gcaattaata tcacaaagta
    10501 gaaatggtaa aattcatgca cttcaaatag ccactgaaag ttctataaga gttacacaag
    10561 aaaggttaaa gaaaaatatt acaggaaaat taaaagaaat tgcttatgaa aaaatagacg
    10621 agcaaacaga ggcacttgtt tctatgattg gcgataatca aattgattat cgttttttta
    10681 ttgggtttaa gcttttagtt acagagaaag aaatgaatat aaagagtata aaaaagtcaa
    10741 tattagctaa tctatcagat tttatatatg atgttaataa taaattgatg gatgactttg
    10801 tcagtataaa taatgatgaa attatgagat ttttaaaaat ggaaaagtta ttagaaaata
    10861 aaatctcaag aagatttaaa gttagaaggt tagataaaaa tgattttgga tatttaattg
    10921 aacataatta tggaaagaaa ggcacagttt ttgaagatta tcaataccat ttacctacaa
    10981 agaaacttaa aagagaaaca ttagtaaaga aatacaatct tataaagcca actagatgtc
    11041 ttatagaaga gaagcaaaga tacttaaaaa ttatacatga ggattcagaa agttttgtag
    11101 cttattttac tattgaaatt attattggag aaatgaattt tccatctagt gagatattct
    11161 attatcagca acaacagttt gcatttccta ttgatactag tatgaatgtg gaaattgtaa
    11221 caaataaaaa ggctttaact acagttagaa ataagaaaaa agaattaaaa gatttagaca
    11281 atcatgcttg gcagtcaggg aatgaaacag taacaaatgt agtagaagca ttagaggctg
    11341 taaatgagct tgtaaataat ttaaatcaaa caaaagaatc aatgtataag ttaagctatg
    11401 taattagggt atcagcagat agcattgatg aattaaaacg ccgttgtgat gaagttaaag
    11461 atttttatga cgatttaaat ataaaattag tacgcctaat gggagatatg ataggatttc
    11521 acaatgaatt tataccttca agcaaaaggt atataaatga ttatattcaa tatgtaacta
    11581 gtgattttct agcaggatta gggtttggag caactcaata gcttggagag aacgaaggaa
    11641 tatatattgg atatagttta gatacaggaa gaaatgtcta tcttcaacca tctcttgcaa
    11701 gtcaaggagt aaaagaatct gtaactaatg cgttggtagc agcattcttt ggatttttag
    11761 gaggtgggaa atcatttgct aataacttac ttgtttacta ttcagtattg tttggagggc
    11821 aagctgtttt ggtggatcca aaagctgaac gtggcaaatg gaaagaaaat ttaccagata
    11881 tatatcatga agtaaatatt gttaatttaa caagtgatat agaaaataag ggaatgctag
    11941 atccttatgt aattatgaaa gaagtaaaag atagtggaag tctagctatt gatatattaa
    12001 cttttcttac aggaataagt agtcatgatg gagagcagtt tcctatactt agaaaatcaa
    12061 tcataaatgt tactcaagac aaatgcagag gacttttgaa ggtaatagat gaattaagga
    12121 aagataattc tcaaatatca aataatattg cagatcatat tgaaagtttt acagattatg
    12181 attttgcaca attacttttc tcagatggaa atattaaagg ctcaattaat ctagaaaaac
    12241 aaatgaatat aatacaagtt gcagatcttg ttttaccgaa taaagatact ttatttgaag
    12301 aatatacaac aatggaaagt ctttcagtag ctatgttgat agtaattagt acttttgcat
    12361 tagattttat ccatacagat agaagtactt ttaaaatagt agatttagat gaagcttgga
    12421 catttcttaa tgtagcacaa ggaaaaactt tatctaataa attagttaga gcaggatgtg
    12481 ctatgaatgc aggagtgtat tttgtaactc aaaatgcagg tgatgtatca gatgaaaatt
    12541 taaaaaacaa tattggatta aaatttgcat tcagaagcac agatatcaat gaaattaaga
    12601 gaatacttga attttttgga gttgataagg aagatgagaa taatcaaaaa aggttaagag
    12661 agttagagaa tggacagtgt ttaatacaag atttatatgg acgagttggg gtaatacaaa
    12721 tacatctagt ttttcaagaa ctccttactg catttgatac aagaccacct ataagaaaag
    12781 aggtggatta atgaaatcga aagtatttag aaatttaagc tggataacta ttttaatagt
    12841 agtactttta ctatttttta tttatataaa tttatagatt tagagaaaca aatagataca
    12901 ttagataaga aaagaaaaaa actcatagat atgagattag aagatataat tgataaagaa
    12961 tcatatgaag aaaagtatga tgatttaaca gttgaaataa agaaacttag taaagagaaa
    13021 aaaactctta aagcatcatt aataaatgaa aagagtataa aagcaagact tagagaattt
    13081 agaaagatac ttgaaagaaa tgaattgtta gaaaaatttg atagatatgt atttgcaagt
    13141 atagtagata aggttatagt tggagttatt aatgaagatg gtatagttga tccttataaa
    13201 ataacttttg tttataaaac tagctttgat gataaacaag atggacataa atttataaat
    13261 aagagtccgt tagaaacgta ttcaaaagat aacaacgata ataaaaagaa gtgttcatat
    13321 actaataatg agacattgcg agactgtagt taaacttatt agagaataac aaggtttcag
    13381 acttttatat tgtgtcttaa aagtagattt gccacctatt tgccaccata attatttttg
    13441 ggtggcaaat ttattctaca atgatttcaa aaatatttac cgtctggttt ctcatgttgt
    13501 tagttacatg taaatatgta tccatagttg ttgaagtttt agcatgtccg agtatatgct
    13561 ggatatcttt tatattagta ccattttcta ataacatagt atcagggttt ttaaaataaa
    13621 gtgtgtattc ttctaaagta gtgataatta aaatatcaaa tatcactata caaaggaaag
    13681 tgcacgcttt ttatgtttaa caaaaacgaa atattaaaaa acttattgaa gaatatgatg
    13741 tcaaaacaac tacagatatt caagatatgc taaaagattt atttacaaat acaattcatc
    13801 aaatatttac attgtcgaat tttagcggtt taaacataat atattgactg atataataaa
    13861 atcatagaat attttagggg gaaatagatt atgtttaaga aaaagttaat agctgttgca
    13921 atgagtataa ctatgattag tgtagggagc ttttcgtatg ctcattcagg aagaactgat
    13981 tcatcaggag gtcatcgtga taataaaaat aaatctggat taggaagtta tcactatcat
    14041 tgtggtggtt atccagctca tttacataat aatggtggtt gtccttatac tggaggagga
    14101 agcagttctg gaacaacaac ttcggttaat aatgaagaaa agcaaaagag gtctgttgga
    14161 gaaaaaggat ataatcaagg gtatgaagat gggtataaag gaaattatag ttcatcaaat
    14221 tatagtggag attatagtga tacatatgaa tcaaagtact cagaaggata tgaaaaaggt
    14281 aaggctaaat tagaagagga agaaaaggtg gctaaagaga ctggatataa tttagggctt
    14341 acaggtgcta aatctaataa tacatatgaa aaagaagttt taaaaaatgc ttatgatacc
    14401 ggatattcaa ctggatacaa tgaatataag actaaaaaga tagaagaata taaagctaaa
    14461 gggatagagg atagcaataa agataaggaa aaaatgacat ttgaagaaaa catagattct
    14521 gaatttatag atgcttataa taatgcttat gacgaaatac aagaacaatt aaaaaatgat
    14581 tacactactc aaggatttga gagtgctatc aaaggagaaa gatttgacac atcaactata
    14641 ggtaatgtta aatatgctaa ttggtttaaa gaaggatatg ataatggtaa ggttaagtta
    14701 cctaaagcaa aagaaagtgt atataatcaa ggttacaatg aagaagattt tagtatacca
    14761 gatgaaatga agtctattga aactaagtta aaaggtgtat atgatgaagg tttagaaaaa
    14821 agagaagaag agaaaagtag aaatgctact tatggggttg gagctggaac agcagctgta
    14881 gttgctggag tagtatataa gaagaaaaaa cgtaaaaaga tatagatgct agatatacca
    14941 atgatgacaa gtgtataaca aatataattt taagtataaa tatttaaaat atagaaggga
    15001 aggttatatc tatgggattt aactttagga aaagcataaa tctaggagga ggactcaaat
    15061 taaatttagg taagaagagt gtaggtataa gtgctggtgt taaaggtgct agagtgtcag
    15121 ttaattcaaa aggaagaaaa tcagctacat tgagtatacc tggaactgga atatcatata
    15181 caaaaactag tacaaagaaa cgtaaaccta aataaatatt tgatatacta agaaatagac
    15241 tgttacaaat agtctatttt ttagtatata gatttaaaaa gtgacaaaat tttgaaatat
    15301 aaactaattt cataaaggaa aatattattt tatgtagaat tataatattt aagtaatatt
    15361 ataaaattta taggaggatt tcattatgaa attaagcaaa atactattgg caatagggat
    15421 gtctgtttca ttattgattg ctagttgtcc aacatcaaac gctcttagtt ctattaattc
    15481 aataaaaggt acaaataagt atgaaacagc aggtttaata gcagataaac aaaattatga
    15541 tattgcaata cttattaatt ctgaaaatgg attatctgat ggattgtcag caagtggatt
    15601 gtcaggagct aaaaatgccc caatattgtt aactaagaaa gatacaatcc cttcagaaac
    15661 attaaaaaga ttagaaaagg taaaagaagt atatctaatt ggaggaaaaa attcgatagg
    15721 agcatcagtt cagaaaatat taagtgataa aaaaataagc ataaatagaa taagtggaac
    15781 tgatagaata gatacaagtc ataaagttgc aaaagaaata ggaagagtaa gtacggttaa
    15841 aaaagtaata ttaactaatg cttttaaagg cgaaccagat gctatgagtg ttgcttctat
    15901 agcagttaga gataaatctc caatagtatt aactgatgga aatactacaa gttttgctac
    15961 agatggatta gaaacttatg caattggtgg aaaagtcagt atgagtgata agttaataaa
    16021 tgaaactaaa gcaacaagaa taggtggagt agatagatac gatacaaata aacaaataat
    16081 aaataaattt tataatggct caaaagaatt ttacatagca agtgggactg atttagttta
    16141 ttctttagtt ggttctacaa ttgcacaaaa aacacctata gtgttagttg gaaatggaag
    16201 taataagagt atattaaaaa atgcaagtaa aataactgct attggaaata ttgatgatgt
    16261 tgttataaaa caatgtttaa atataactaa taatgttggg gattcaaata caggtattgt
    16321 aaaagatact aattcaaata atacgaacaa accaagtaat gagcctagct ctaataatcc
    16381 tggattaact gataaaagta cagtatatgt aactcctaat ggaaaatcat atcatctaac
    16441 aaaaaattgt tctacactaa aaagaagtaa aacaataaat aaagtaacat taaaacaagc
    16501 taagtcacaa ggtaaaatag atccttgtaa tgtttgtgta aaataatgta ttagccaaat
    16561 atatattaaa aaagacaatt aattaagatt gtctttttta ttgaagaaat ataaatttat
    16621 attatgtatt attaaaaaaa aattattaat tataaaaaat aataataaag acgataagta
    16681 ttaaaatttt atgtttccaa tatttatgat ataattaata tggcaaggaa taaatatttg
    16741 aaaaagctat gagtggtgtt tccatggaag tttcttcact ttcttatgag aggaggtgaa
    16801 aactatggaa aatctaatga tgagtattat aactggtgtt atagccagtt acatttacga
    16861 taaattaaag agccactctg agcgaccaac taagagtggc tgggaactta atataaagtt
    16921 ccagaaaagt aaatcttcaa aataatttgt tgaaggaaac atcactcaaa gttaagtaaa
    16981 atatgtttcc ttgcttttat tatatcacaa attaaaaata ttaacacaaa attttgtgaa
    17041 tatatactta ttattgttct aaaaaaatat gtaaataatt gatttgattg catggaatta
    17101 ttgatgtaat atttaagtta aatatacaca atctcaatta aagaagataa gtactataaa
    17161 ttcatatcaa aaaaatacat aaacttttaa gttataattt tataaaaagt cttttagtat
    17221 cattatttca agaggaatta tcatatgaaa aaataacttc attagaaaag tggataaaaa
    17281 actggtaaga agatgactaa agattacata tactattac
//
LOCUS       DAYASV010000084        17311 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYASV010000084 DAYASV010000000
VERSION     DAYASV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17311
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.084"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(305..697)
                     /locus_tag="ACGI68_002559"
     CDS             complement(305..697)
                     /locus_tag="ACGI68_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0138989.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1264..1626
                     /locus_tag="ACGI68_002560"
     CDS             1264..1626
                     /locus_tag="ACGI68_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138990.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSKIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1610..2335
                     /locus_tag="ACGI68_002561"
     CDS             1610..2335
                     /locus_tag="ACGI68_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138991.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDVSNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEILNQFKNESENIRNQEVGGFTEEDEEDEEYEEELKQN
                     IIENIKKNFDEKNLVQVYKYRGV"
     gene            2328..2774
                     /locus_tag="ACGI68_002562"
     CDS             2328..2774
                     /locus_tag="ACGI68_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138992.1"
                     /translation="MYNFEKRRNVTVIELIVSISIILLIVTLSFPKDNLENHKINLFA
                     RQLCSDIRYVRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSI
                     EIIKFKSDGSPQKASTIEIYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2764..3288
                     /locus_tag="ACGI68_002563"
     CDS             2764..3288
                     /locus_tag="ACGI68_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0138993.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKRSQKYE
                     TDFKIKVWNFSESI"
     gene            3600..4157
                     /gene="efp"
                     /locus_tag="ACGI68_002564"
     CDS             3600..4157
                     /gene="efp"
                     /locus_tag="ACGI68_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFH0138994.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4249..4704
                     /locus_tag="ACGI68_002565"
     CDS             4249..4704
                     /locus_tag="ACGI68_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1247 N-terminal domain-containing protein"
                     /protein_id="HFH0138995.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4867..5577
                     /gene="rnc"
                     /locus_tag="ACGI68_002566"
     CDS             4867..5577
                     /gene="rnc"
                     /locus_tag="ACGI68_002566"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFH0138996.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5631..6701
                     /locus_tag="ACGI68_002567"
     CDS             5631..6701
                     /locus_tag="ACGI68_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006448 - regulation of translational
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongator complex protein 3"
                     /protein_id="HFH0138997.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6729..10283
                     /gene="smc"
                     /locus_tag="ACGI68_002568"
     CDS             6729..10283
                     /gene="smc"
                     /locus_tag="ACGI68_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFH0138998.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKFAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10310..11590
                     /gene="ftsY"
                     /locus_tag="ACGI68_002569"
     CDS             10310..11590
                     /gene="ftsY"
                     /locus_tag="ACGI68_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFH0138999.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDIEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11693..12061
                     /gene="ylxM"
                     /locus_tag="ACGI68_002570"
     CDS             11693..12061
                     /gene="ylxM"
                     /locus_tag="ACGI68_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HFH0139000.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12058..13410
                     /gene="ffh"
                     /locus_tag="ACGI68_002571"
     CDS             12058..13410
                     /gene="ffh"
                     /locus_tag="ACGI68_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFH0139001.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPINIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13448..13720
                     /gene="rpsP"
                     /locus_tag="ACGI68_002572"
     CDS             13448..13720
                     /gene="rpsP"
                     /locus_tag="ACGI68_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFH0139002.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13785..14012
                     /locus_tag="ACGI68_002573"
     CDS             13785..14012
                     /locus_tag="ACGI68_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFH0139003.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14120..14635
                     /gene="rimM"
                     /locus_tag="ACGI68_002574"
     CDS             14120..14635
                     /gene="rimM"
                     /locus_tag="ACGI68_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFH0139004.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVAREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14644..15339
                     /gene="trmD"
                     /locus_tag="ACGI68_002575"
     CDS             14644..15339
                     /gene="trmD"
                     /locus_tag="ACGI68_002575"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFH0139005.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15524..15874
                     /gene="rplS"
                     /locus_tag="ACGI68_002576"
     CDS             15524..15874
                     /gene="rplS"
                     /locus_tag="ACGI68_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFH0139006.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16195..17091
                     /gene="ylqF"
                     /locus_tag="ACGI68_002577"
     CDS             16195..17091
                     /gene="ylqF"
                     /locus_tag="ACGI68_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFH0139007.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
BASE COUNT         7452 a         1563 c         3197 g         5099 t
ORIGIN      
        1 tacacattaa tatctataaa gtgacaaaat aaaaaatact atttaaatag atattaaaaa
       61 attgatacca agtatttatt tgctatatga ataacaattt atttaggaac tttactttaa
      121 cactgtataa taataaagta attgctaaaa acgtttgatg atttggatgt atttttagaa
      181 attagaaaaa tatatactta ctgactaatg ataatcttat atgtatttta gtttgagtga
      241 tttaagaaaa gtgttttgaa tggggattat tttatagagt aaataaatat ataatagtag
      301 aaaattacat ataaagagta gtgtttattt cattaccttc tttatctgtt ccgatagggt
      361 cttgtagaga tacttgagtt ttattttttt tgttagatct aatattcatt aaaatctcat
      421 tttcaataca tctagaagca tatgttgcta atcgagttcc tttatctatg ttataagttt
      481 ctatagcttt aattaatcct attgtaccta tggatattaa atcatcctga tcttccgttg
      541 aattattata tttctttgct atataagcta ctaatctcat gtttctttca attaatgtat
      601 cttttgcact tttatcgttt tctattttaa atttagttaa atactcaatc tcttcttcag
      661 gggttaaggg tttttcaaaa gatttaagag ctgccaatag gtcaccccct tttcaacggt
      721 cactttaaat tatatgaagg agacttatcc aagttgactg ttatctaata aaataatatg
      781 catattttta gaatttaaaa catatatttg caaacttttg aatggaatat gacataaaga
      841 gtgggaattt aaatttaagt ttttgttgta tatatatgaa ataaggcgaa gaaattattg
      901 atttataaag tataaattgg aattgaggtg tcaataaata taaaaggaaa tagtatttat
      961 atatgttttg gtgttgaagt aaaagattaa tgcaaagcat tagagtctat aaaaatatga
     1021 atataggagg tgtaatttta caatacttaa aaaagtaaat gtaattaatt gtttgtataa
     1081 agttgattac aagcaaaagt atcagtcata gttaatactg ttaagggtga aggtggtgtg
     1141 ttaatttata tatgtatggg aagaaatttt gattacaatg aaaaaactta caaaagatat
     1201 ttatgaaaaa acttatagtt aaatgtttat atataaaata ttatttaggg gttgttttta
     1261 ttattgaaaa taagaaagag tggttttata tcaattgaat gtataataag tattgctata
     1321 ttatatgtgg ctgtttattt agtttctaca tcattgtata attgttatag ttttatcagt
     1381 agaaatatat ctgacagaga aatgttaagt acagcaaaaa aatatataga agatgagaag
     1441 tatagaatac aaaatagtaa gtatgagtta attgaagata agatagaaaa aaattacata
     1501 aatggatatg aaattaacag taaaatagag caaattttag attattatca atgctatgaa
     1561 ataaatatag agataaaaaa tgaatttaaa aaactgaggt ttaatagcta tgttactaga
     1621 aaataaaggc tctatattaa taataacatt gataatattt tctattataa gtactgtgtg
     1681 cattatgtgt ataggtttaa tttatacaca taataatatg tttgagttag gatacaagga
     1741 cattcaacta aaagaaattt ctttaagtgg aatagaaata tcagtaagta atgtactttc
     1801 ttgtgttgag gaagctattg atgtatctaa taatgaagac gaatttagag aatatttctt
     1861 aggaaataat atgattgaat gtataaaaaa aataaaagat acatcttata gtagtttaga
     1921 agaactttct ttaaaagtag aaaataatac tatttatgac agaggagatt tttttgaatt
     1981 tgatatagaa agtaatgtta gagaaaaaga atttttaaaa gcaataaaag taagagtgaa
     2041 aatcaaaaat ccatggatag gaattgacat tgaagattat atggataaat ctaataacaa
     2101 taaaattgat gacaaaacat tcaataatta taaagcatct gaaaataatg aaattctaaa
     2161 tcaatttaaa aatgaaagtg aaaatataag gaatcaagag gttggtggat ttacagaaga
     2221 agatgaagaa gatgaagaat atgaagaaga acttaaacaa aacataattg aaaatataaa
     2281 gaagaatttt gatgaaaaaa acctagtaca ggtatataag tataggggtg tataactttg
     2341 aaaaaagaag gaatgtgact gtaatagaat taatagtatc tatcagtata atactcttga
     2401 tagttacatt atcttttcca aaagataatc ttgaaaatca taaaataaat ttatttgcac
     2461 gtcaactttg ttcagatata aggtatgtta ggcgaattaa tatgtttagc aactacaata
     2521 catatatata ttatataaat caaggtaaac acgaaggtta tgtattgaga aaagatggta
     2581 aaaacattaa atcaataatg ctacctaaaa atgcaaaaat agctaggagt attgaaataa
     2641 taaagtttaa atctgatggg tcgcctcaaa aggcgtctac aatagagata tataataata
     2701 gattaagaaa aactataaca ataacacctg taagtggaag agtacttctg aaagagggaa
     2761 aatatgaaac ataaatatgg ttacttgctt ttggaaagtg tcgtatcatt gtcttcaatg
     2821 gtgataatta tactagtttt atattctata tttttgtcta caataaactt aaagttaaaa
     2881 gtagaagata agatagaact acaacaacaa agtcttgaaa tcataaaatc tatggaagga
     2941 attattagca attctatggg aataatgaat gtatctaatt atgaagaaac atttaaaaag
     3001 acaacatcta taaaatgtag atatttagat gaaaataata atgaagaagg cataagtaat
     3061 aaagaaatta tattgaatga aagacgaaat aagttatttg taaattcttt aaatggcgaa
     3121 agtagtcaag caggtggtta tgaaattgga gattatgtag atgagatgta tgtatcaatt
     3181 actaataatg gtcaatatgt taatattaaa ttgaaattat caaaacgtag tcaaaaatat
     3241 gagactgatt tcaaaataaa agtatggaat tttagtgaaa gtatatagaa atacataaat
     3301 ttaagagaaa gaagtgacat caataagtca tataaaatat aattattgag aactatataa
     3361 ctaaataata tcaatatgta aagtgtatag attaagaata tagaggataa taataaaaat
     3421 atagaataag taatttgcca aatacttgtt tatattgtat aacttatgat attatgatat
     3481 tagtaaaaat ttgtaatcct gacttgacag aattcaagta agagatataa ttttaaaggc
     3541 atagtaatgt aaaatttact aaaaaataag aaatttattt taatatggag gaacaaaaaa
     3601 tggtatcagc aggtgatttt agaaaaggtg ttacatttga aaaagatgga caaccatgtt
     3661 tagtagttga ttttcaacac gttaagccag gtaaaggagc tgctttcgtt agaactaaat
     3721 acaaaaactt aaaaacagga gctataagag aagaaagttt caatccaagt gaaaaattcc
     3781 ctaaagctgt tatagataca agacaaatgc aatatctata caatgatggt gagttatatt
     3841 attttatgga tcaagaaaat tttgaacaaa taccattaaa ctatgagcaa gttgaagatg
     3901 caattaagtt cttaaaagaa aatgaagttg ctacaataag attttaccaa ggacaaccat
     3961 tccaagtgga agcgccaaac tttgcagagc tagaagttac agatacagag ccaggtataa
     4021 aaggtgatac agcaagtaat gtaactaaag cagctacagt tgaaactgga gcagttgttc
     4081 aagttccatt atttataaat actggagata aagtaaagat agatactaga acaggtgaat
     4141 atttatcaag agtataaaaa tttatgtata taaaagctta tatgtcttaa taatataaga
     4201 tatatgagct tttagtttat atataaaaaa gaaagcgagg ggtgttacat gaaacattta
     4261 tatgaagaag ttgcatactt aaaaggccta gcagaaggac tagaaataag tgctgaaagt
     4321 aaagaaggga aaatgataca taaaatagtt gatgcattag aagtatttgc agatgctatt
     4381 gtaacattgg atgaagaaca agaagaactt caagattttg ttgaatctat agatgaagat
     4441 ttagcagatt tagaagaaga tattgctgac atggaagaag atctttatga agaagatgac
     4501 gatgaggatg atgaggactt cagctatata gaaatggaat gcccaaattg tggagagtta
     4561 gtagaaatag acgaagattt gttatatgat gatgaagtag atgttgtttg tcctgattgt
     4621 aaggctgtga tattatcatc agaagatgat tgtgatgatg attgtacatg tggtggttgt
     4681 tcaagttgtg atgatagaga gtaaaaattc ataagaatta aaactgccta tgatgcctat
     4741 gagatatttt gcttcatagg tagttttatt tttatgagga agggtattat gtattttggt
     4801 tttgcaatac ttggacaaaa atagtacact agaatttata gaataggatt ttggagggat
     4861 gtaaatatga aaataagtaa aaaattacta gataatatac aaagatttga aaatgttata
     4921 aattataaat ttaaaaataa agaatatata ctagaagctc ttactcatag ttcatattct
     4981 aatgaaaata aaaaatataa ttttaacgaa agattggaat ttttagggga ttcggtttta
     5041 ggcatagtga taagtgatta tttatttaat gaagaagcta atctgcccga aggggaacta
     5101 actaaactta gagcaaatat agtttgtgaa gattcattaa gtgaagtagc taatgatata
     5161 aatttaggta ttcacatgtt attaggtaga ggagaagaag caacaggagg aagacatagg
     5221 acttcaatat tagcagacgc atttgaagct gtaatagctg ctatatatct tgatggaggt
     5281 tttgaaagtg caagacagtt tatacttcat catatggaaa atataattta tgattcaaga
     5341 aaaggaaata tatttagaga ctacaaaacg catcttcaag aggtgttaca agggaatgga
     5401 gaaaataata tctggtacag gcttatagaa gaaaaaggac cagaccataa taaaagattt
     5461 gtaatggaag ttggaattaa tgacgatgta cttggtatag gagaaggcaa gagtaaaaaa
     5521 gaagctgaac agttagctgc taaaatagct ttaaagaaaa aattatggga aaaataaata
     5581 taatatgtaa aaatataaaa agaaatattt atattagaag gatgttgtag atgaaaaaga
     5641 gaattatacc tatatttgtt cctcacaaag gatgtcctca tgattgcata ttttgtaatc
     5701 aaaaaaaaat aacaggtgtg agtactgatg ttactagtga agatgcaaga aatataattg
     5761 aggaatgttt agagactata gataaagatg cagatgtaga aatagcattt tttggtggta
     5821 gctttactgc tatagatatt gatatacaaa aaagtttatt atcagttgct aaagaatatg
     5881 tagaaaaagg tcttgtaaaa gatattagaa tgtctacaag accagattgt attgatgaag
     5941 atatattaag tatgttaaaa gaatataaga caagtataat tgaactagga gtacaatcct
     6001 tagatgaaaa agtattatta gatagtataa gaggtcatca atctgaaatt gtatacaaga
     6061 gttcaaagat gataaaaaat agtggaatca aacttggatt acagatgatg attggtcttc
     6121 cagctgacac agaagaaaag tgtatattta cagcaaaaaa atttgtagag ctaaaaccag
     6181 attgtgttag agtctatcca actttggtag taaaagatac aggtcttgaa aagttaatgg
     6241 aacaaaataa atataatcca tttactcttg aagagagtgt aaatatagta aaaaaagttc
     6301 ttgtactgtt ttatgttaat aatatcaatg taataagagt tgggcttcaa gctactgatg
     6361 atatacaaat tggaaaagct gttttagcag gaccatatca tcctgcattt agagagttgg
     6421 tagaagcaga tatgataaaa gattatttag aatttgttat tttgcaaaat aaaaatataa
     6481 agcaaatgtt ggtcaaatct aataaaaaga atatttctaa aatcatagga aataaaaaaa
     6541 caaatgttaa atatatggaa gaaaaatttg gtgtgctttt aaaaacacaa gaatcagatt
     6601 tagatataaa tcaattagaa attgtgttag atgggaaaag tttaattagt gctaatatga
     6661 gagacattca tagaaaattg tatgacattt atgaccttta gttttaataa tgaagggaga
     6721 gaaaagattt gtatttaaaa agactagagt taaagggatt taaatccttt ccagtaaaaa
     6781 cagatattat ttttaaagaa ggaataacag ccatagtagg accaaatggg agtggaaaaa
     6841 gtaatatatc tgatgctgta agatgggtat taggagaaca gagtattaag agtcttagag
     6901 gtgataaact cgaagatgta atatttgcag gtacagatac aaaaaaacct atgaattatt
     6961 gtgaagtggc tcttactatt gataattctg aaaatcaatt ggaattagat tttacagaag
     7021 taactataag aagaagggct tatagaaatg gtgaaagtga attttttcta aataataaaa
     7081 gttgtagatt aaaagatata aaagaagtct ttttagatac tggtattgga aaagatggtt
     7141 attcaatcat agaacaagga aaagtagatg aaatattaag taataatcct ttaagcagaa
     7201 gaaaagtttt tgatgaagct tgtggcatat caaaatatag atataaaaaa caggaagcag
     7261 aaaggaattt aagtaataca aaggaaaatt tagaaagaat agatgatgta tatatagaaa
     7321 ttgaaaatca attaaagcct ctttttaatc aacagacaaa ggcaaaaaag tacttggaaa
     7381 taagtgaaaa attaaagaca cttgaagtaa atagctttat aagagaaatt gaaggaatag
     7441 aaaaagaatt aagtgaggtt aatgaacatc gtaaggttat tgaaaaggag ctcaatgaaa
     7501 aagaagaaca aaaaaatgtt gtagagaaaa agcaagaaga tattaataaa gaggtagagg
     7561 tattacaaga tgtaattgaa aaatctgtag attatataaa ttcaattaaa ggagttatat
     7621 caaaaaaaga gtcccaaata aatttaatta aggaaagaat aagaaatttt acgaatgaaa
     7681 ttagtagaaa aaatttagaa ataaaggata taaaagagaa gctaaatgaa aataagcaat
     7741 acataaaaga attagaaagc aataaattat caggtagtga agaactatct accttgcagg
     7801 aaaatataaa agtattggaa ggaagtaaag ataaacaaaa aattaagtta gaatccctaa
     7861 ataatgagat agaattattg aaagaaagta taattgatat attaaataag aaacaagagt
     7921 ttagtaataa gctgtctact ttaaacgcaa ataaagaaaa tatgaatatc agagatgaaa
     7981 atataaattc agagataaca gagttaaaca aaaatattga aataaaatca tctgaattag
     8041 atactataaa taaagaattt aatatgcaaa atgaaaattt aaagaatgta aataatagac
     8101 ataaagaatt atcaattaat ttgcaggatt caattagtga acacaataaa ctagaagatg
     8161 aaatacagaa aagtaaatat aatttaaatg gatataattc aaagttaaat gtctatatag
     8221 atatggaaaa tcattatgaa ggatttaata gaggagtaaa agaagtacta aaaaataaaa
     8281 atttaaaagg agttcatgga gcattaggac agattataaa tgtacctgaa aagtatgaga
     8341 aatctataga agcagctctt ggtgcttata tgcaaaatat aataactgat aatgagttta
     8401 gtgctaagtt tgcaataaac tatctaaaac agaataattt aggtagagta acatttcttc
     8461 cattaaatat aattaaatca aataaaataa gtctaggaaa tttaaaagca aatactaagt
     8521 tcataggaat agcaagtgat ttaattacat ttgatgaaaa gtatagaaat ataattgaaa
     8581 atatacttgg aagaacaata cttataaata atatagatga gggcataaaa tttgcaaaag
     8641 aaactggtca taggtttaaa atagtaactt tagatggtga aatcttaaat ccaggaggtt
     8701 ctttgactgg gggaagtcta aaaactaatg ggaatatact atcgagaaaa agatacatta
     8761 atgaatatac agaaaagata agtaatatca agaatgaaat ttcacatcta gaattaaaaa
     8821 gagaatcttt agacaaagat gtgaaaaata tcaaaaatga aattgattca catgaaagta
     8881 aaataaaaga tttagaaaaa agtataataa taaaaagtac aagtataaag aatgttgagt
     8941 ctgaaattga atcgttaaaa ggtagtatta ctaaattaga aaatgagaag aatgatttaa
     9001 attctaacct aaattataca cttgaaaaaa gtgatgatgt tagaaaagat atggaagagt
     9061 tagatgattt atacaataaa aataaagaaa aaattgatgc attgaatgag gaaataaaaa
     9121 gatacaatga tttatatgat aaagaaaaat ctgaatttga tgaattgaac ttaagtttag
     9181 tgaagaaaac agaagtttac aatagtatag ttagagatat aaaaagaata tctggtgaaa
     9241 attgtgagct agaagaaaaa aataaacaat tagaagagtc attaaattat gaagaacatg
     9301 aaataattaa attacaagat tctatactca ctgaagaaaa agaaaaagaa aatttaacaa
     9361 aacaactagg ggatagtaat agaaatcttg aaactagaaa aatagcaaaa gatgatttaa
     9421 aaaatagttt tgatgaaatt aataaagaat taaaaactat agatagacag catatagaac
     9481 ttaaagaaag tttatttaaa gttggtggaa gattagaaag attaaaaact agtcaagaca
     9541 catatataaa taagttgttt gagcaatatg atatgacctt agtacaagca ttggaaatta
     9601 aagatgaaga tttggatata gatagaaaat tcttagaaag tctaaagaga gaaataagaa
     9661 gtcttggaaa tataaatata gactctataa aggaatatga ggaaataaaa gaaagatacg
     9721 atttttatag cgaacaaaag caagatttag aagagtctat ggaagagata gaaaaactta
     9781 tacatacttt agaagaaaat atgaaatctg aatttgaaat taagtttgaa gaaataagta
     9841 aaaattttaa atatgtatat aagagattat ttggtggagg ttgtggagaa ttaactattt
     9901 tagataaaga aaatttatta gaaagtgata tactaataac agctcaaccc ccaggtaaaa
     9961 aaatgaaaaa tttaaaccta ttatctggtg gagagaaagc tttaacggca ataagtatat
    10021 tatttgctat attgattaca aagccaacac cattttgtat attagatgag attgaagcgc
    10081 cacttgatga cgcaaatata tttagatttg gagaattttt gaaggattta tctaaagaga
    10141 ctcaatttat atcagtaact cataggagag gaacaatgga agctgctgat tatatctacg
    10201 gagttacaat gcaagaaaaa gccatttcaa aagttattag tttaaaatta aaagaagctc
    10261 aggaaataac agatattata taacttagat tgacatagga ggaataacag tgttaaaaaa
    10321 attatttggt tttggaaaag acaaggaaaa ggaaatagaa aaaaaagatg ctgaagaaga
    10381 aatagaagtt gaagacagtg ttgataattt agaaaacttg gaagaaacta ttttttcagg
    10441 acttgaagaa gaagtgatag ataaagtgga agatattgaa gaaaaaaatg aggaaagtgt
    10501 caataatgaa ggcactgaag aaatcgaaaa ctttgaaaaa atagaagttg agatggatag
    10561 taatggaaaa gaagtagaaa atatttcaaa taatgattct caagaagaga taatcgaaga
    10621 attagaaaat tatgatgaaa ttgagtctaa agagatagaa gataaagaag ataagaaagt
    10681 taacttgttt gaaagactaa aacaaggatt gacaaaagca aagcaaggaa taactgatag
    10741 aatagatgaa gttttaaaat catatacaaa aatagatgaa gaattacttg aagatttaga
    10801 ggaaatttta ataacagcag atgttggtgt aaatacaact atggatataa ttgaaagact
    10861 aagagataaa ataaagcaaa aaggtattac tgaaccaata aaggtaagag aagaacttaa
    10921 atctatagtt gaagatattc ttacaaatga aaactctaca ctagatatag aaccagcacc
    10981 atgtataata ttaatggtgg gtgtaaatgg ggtcggtaag actactacaa taggtaaact
    11041 agcaaataga tacaaaaaag atggtaaaaa agttttatta gcagcagcag atacatttag
    11101 agcagcagcg acagagcaat tagaaatttg ggcaaataga accaatgtag acataataaa
    11161 acatcaagag ggagcagacc ctggagctgt agtatttgat gctataaaag ctgcaaaagc
    11221 tagaaaaaca gatgtattaa tatgtgatac agcaggaaga ttacacaata aggctaatct
    11281 tatgaatgaa cttggaaaag tatttaaaat agtagataga gaatttccag aagcaaaacg
    11341 tgaagtactt cttgtggtgg atgctacaac aggtcaaaat gcagttgttc aagcaaagac
    11401 atttaaagaa gttgcagata taacaggtat agtacttaca aaacttgatg gaactgcaaa
    11461 aggtggagta gtccttgcag taaaatcaga agtagatgta cctgtaaaac ttattggagt
    11521 tggagaaagt gtagaagacc tacaagattt caatgcaaaa tcattttcag atgcattatt
    11581 tggtaactaa tgttctttta aaaataatgg ttgacaagag tgttgttatg atataaaata
    11641 caatagtgta aagtaaataa cctttacagt atactgctag gaagtgtaag atatgaatat
    11701 agaaaaaatg gtcgaaattg gattgttatt tgaacagtat aaagaattat taactgacaa
    11761 gcagaaagaa atagttgctc tatactatga agaagattat tctttaggtg aaattagtga
    11821 aaatctgaat gtatcaagac aaggtgttta tgatacctta aagcgttcag aaaaaatact
    11881 aagagattat gaagaaaagt tacacttagt atcaaagatt caagagcagg aaaaaaatat
    11941 taagattata aaagataaaa ttattgacat taaagaagat ttattgcaca atagagattg
    12001 tgctaattta atccctaagc tagaaaatat agaagatgta tgtagggaga tgataaaatg
    12061 atatttgaag gattgtctga taaactacaa ggggcattta gtaagttaaa atctaaagga
    12121 aaacttacag aagtagatgt aaaaaatgct atgagagaag ttaaacttgc tcttcttgag
    12181 gcagatgtta actttaaagt agttaaagat ttcataaaaa aagttcaaga gagatgtgtt
    12241 gggcaagaag ttatgcaaag cttaactcca gctcagcatg taatcaaaat tgtaaatgaa
    12301 gagcttacaa gtttaatggg agatgtacaa agtaaagtaa tgatttcctc aaagcctcct
    12361 acaattttaa tgatggttgg attgcaaggt gcaggtaaga ctactacagc aggaaaactt
    12421 ggaggatatt ttaaaaagca aggtaaaaaa cctctattag tagcttgtga tatttataga
    12481 ccagcagcta taaagcaact tcaagttgtt ggagaaaaat tggatatacc tgtattcaat
    12541 atgggagata aagaaaatcc aataaatatt gcgaaagcag gtttaagcca tgcgattaaa
    12601 aatgctaatg atttagttat tatagataca gctggtagac ttcatataga tgaagtactt
    12661 atggatgagt taaagtctat taaatcagaa gttaagccac atgagatact tttggttgta
    12721 gattctatga caggtcaaga tgctgtaaat gttgcagaaa gcttcaatga ggcacttggt
    12781 gttgatggag tagtattaac taaattggat ggagatacta gaggtggtgc tgcgctttca
    12841 ataagagcag ttacacaaaa gcctataaaa tttatgggga tgggagaaaa gctagatgat
    12901 atagaacctt tccacccaga tagaatggca tcaagaatat taggtatggg agatgtctta
    12961 agtttaatag aaaaagcaca agaaaatata gacttagaga aagctaaaga attagagcaa
    13021 aaaattaaaa aacaagagct tgattttgaa gatttcttac aacaaatgga acaaatacaa
    13081 aatatgggac ctcttgacaa gatacttggt atgatacctg gtatgggaaa tgtaaaagac
    13141 caacttggag atgtagattt gaatggcaaa gaaatgaaga gaacaaaagc cataattcag
    13201 tcaatgacta catatgagag aagaaaccca ggagtattaa atgcttcaag aaagaaaaga
    13261 atagctaaag gcagtggaac tagtgtacaa gatgtaaata gacttataaa acaacttaat
    13321 gaaatgaaaa aaatgatgaa gatgtttgct ggttcacaaa agagtatgaa gaaaaggggc
    13381 ggcttaggcg gtttaccttt ttttaaataa aatataaaaa agtaaaaatt attcggaggt
    13441 gactgtaatg gcagttaaaa taagattaaa aagaatggga gctaacaaaa aacctttcta
    13501 cagaatagta gtagctgatt caagagctcc aagagatgga aaatttatag aagaaatagg
    13561 atactataat ccaatttctg aacctaaaca agtaagaata aatgatgaaa aagctataaa
    13621 atggttagct actggtgctc agccaacaga agtagttaaa aaattacttg taaaaaacgg
    13681 agtaatagaa aaattcgaag cttctaaaca agcaaaataa ttttttcttg gcaattagtt
    13741 taagtaaggg ctcattgcta aggttacttt aggagatgaa atatatgaaa gagctagttg
    13801 tagatatagc taaggctcta gttgataatc ctgactcagt tgttgttgag gaatttgaag
    13861 ataatgatgg tatcgtctta aagcttacag ttgctcaaga ggatatgggt aaggttattg
    13921 ggaagcaggg aagaatagct aaagctataa gaactgtcgt aagatctgtt gcaaatagag
    13981 aaaatataaa agtttctctt gagatagtat aaaataggag gattaggtat aatgcctagt
    14041 cctttttata taaatgcgta gtttaagatt ttatgatgat atgaccacat aagattaaat
    14101 tttttaagaa ggtgataaca tgaaagaaaa attaactcat tttaaggtag gtcaaatagt
    14161 aaatacacaa ggtttaaaag gtgaagtaag agtttatccg cttactgatg atatagagag
    14221 gtttgatgaa ttaaaagatt tctatctggg aaaagattta aataataaat tggctgttga
    14281 aaaagtaaga tacaaaggtt ctatggttat aatgaagata aaaggcatag acagcataga
    14341 gaaagcagaa aaactaaaaa ataaatttat gtatgtagct agagaagata gcagagagct
    14401 tgaagaaggc gaatttttta tagcagatat gattggaatg gaagttttaa ctgtagatgg
    14461 aaaatatgta gggatactag aagatgttct tcaatactca gcaaatgatg tctatgtagt
    14521 aaaaggtgag gaagataaag aatttatgat accagctata aaaaaatttg tacctacaat
    14581 agatattgat gaaagaaaaa tgattataga ccctattaag ggaatgatag attaggtggt
    14641 aatatgagat ttcatataat gacacttttt ccagaaatat ttaattcgta catggatgaa
    14701 agtataatga agagagcagt tgaaaaaggt ataattgagg ttcatatata taatataagg
    14761 gatttttcta ataataaaca taaaaaagta gatgattatc catttggagg aggagcgggg
    14821 atggtaatga ctcctcaacc tatatatgac acatataagc atataataac aactcataat
    14881 ataaataacc caagtgtaat ttatcttact ccaaaaggta aggtatacaa tcaatctatg
    14941 gcaaagcaga tgtctttaaa agaagatata atactactat gtggtcatta tgaaggaata
    15001 gatgagagaa tcattgattt aatagttact gatgaaattt caattggaga ttatgtatta
    15061 actgggggag agctcccagc acttataatg atagattcta tatcgagatt aataccaggt
    15121 gtattaaacc aagaagaatc atttgaagaa gaatcattta aagacaactt attggaatat
    15181 cctcattaca ctagacctag agattttgaa ggattaaaag taccagaagt gcttttatca
    15241 gggaatcata agaagataga tgagtggaga agagaagaat ctataaggat aaccaaagaa
    15301 agacgatttg atttatataa aaaatctaat gaaaaataaa aatataatga aaaattaata
    15361 tatatgtata caataaagta cctataaatg gaattaaaaa cataaaagtg cttgtaataa
    15421 ctattgaagt atagtataat ataaaatgtt gaaaatacgg gcgttcctct gtttacactg
    15481 gtcgggtagt taagaacgtc tatgatatct aggaggaaga aagatgaatg aaatgctaag
    15541 agcaattgag caagaacaat taaaaaatga agttcctaat tttggacctg gggacacagt
    15601 taaagtacac gttagaatta tagaaggaaa aagagaaaga atacaagtat tcgaaggtgt
    15661 agtattaaag agacaaggtg gaggagcaag agaaactttc acagttagaa aaatgtcttt
    15721 caatgttggt gtagaaagaa ctttcccagt tcactctcca aaaatagaaa aaatagaagt
    15781 tactagaaaa ggtaaagtaa gaagagctaa actaaactac ttaagaggta gagtaggtaa
    15841 agctgctaag ataaaagaag ctagaaataa ataattaaaa actttactta taaatggggg
    15901 gctgatttat cagtcctctt tctattttaa tgaaaattac agcaaataat tatactagaa
    15961 ataaatagaa atatttgttg aaaatataca ttatttatct atgaatttat agcttttaaa
    16021 atgaaaaata aaattggtaa atattgcatt atattttgta acaatattaa aaataagaga
    16081 acagtattat gtaaagtatg acgttaaaaa gatgacaata ttatagtttg ttaagataga
    16141 ttttttaagt ggaattagga tataataata taatattaat aaggagtgat acatgtgtca
    16201 aatgaatacg aagagtatct tatgaatgac aatctacata taaactggta tccaggtcat
    16261 atgaaaaaaa ctaaggagtt agtaaaaaat aacttaaaat tgattgatgt agtaattgaa
    16321 cttttggatg ctagaatccc gtttagtagt aaaaatccag acatagacag gcttgtagga
    16381 gataagccaa gagttgtcgt acttaataag agtgatatgg cagataggga taaattgaat
    16441 caatggattg aatactataa gaaaattaat atcaaagcta taccagtaga tacaataaaa
    16501 ggcgttggta taaataagat aatcgaagaa tgtaaaaatg taactagaga aaaaatgagt
    16561 tctttaaaag acaaagggag aaaagaaaga gctatacgaa taatgatagt tggagttcca
    16621 aacgttggta aatcgtctct tataaacaaa ttaacaggta gaaaaagcac tcaaactgga
    16681 gataagccag gagtaacgaa agggaaacaa tgggttagat taaagggaaa tcttgaactt
    16741 ttagatacac ctggaatact ttggcctaag tttgaagacc aagaagttgc attaaattta
    16801 gcatttagta gagctataaa ggatgagatt ttagatactg aaactttggc tttaagactt
    16861 atagagaaac ttatgaaaat agaacctgag aagttgaaag ccagatataa gttagattgt
    16921 ctaggagaaa ctccaattga aactatggat atgattggcc ataaaagagg gtttataaca
    16981 ggtaaaaaag aactagatta tacacgcata gccacaactg tattaaatga atttagagat
    17041 ggaaaaatag gaaatataac attggaagtt ccagaaaacg ttaaaagata gattttaaaa
    17101 caaaatcaaa aatttaatgt taaaaattta aaaaagcctt ttagcaaagg cttttttatt
    17161 tttttgaaat atagacaaat atctccatat aaaagtataa attaggttag aaaaccttta
    17221 aaaatattaa tttaagacaa atatacctaa tattttgaaa attaaaatga gtgtgattat
    17281 ttaaaatttt agcatattga cttgcttgtt t
//
LOCUS       DAYASV010000085        17290 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYASV010000085 DAYASV010000000
VERSION     DAYASV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17290
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.085"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            52..1119
                     /locus_tag="ACGI68_002578"
     CDS             52..1119
                     /locus_tag="ACGI68_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFH0139008.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1138..2298
                     /locus_tag="ACGI68_002579"
     CDS             1138..2298
                     /locus_tag="ACGI68_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0139009.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVATILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2311..4407
                     /locus_tag="ACGI68_002580"
     CDS             2311..4407
                     /locus_tag="ACGI68_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HFH0139010.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNGLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4566..4910)
                     /locus_tag="ACGI68_002581"
     CDS             complement(4566..4910)
                     /locus_tag="ACGI68_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0139011.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5202..6092
                     /locus_tag="ACGI68_002582"
     CDS             5202..6092
                     /locus_tag="ACGI68_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0139012.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEIIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6310..7596
                     /locus_tag="ACGI68_002583"
     CDS             6310..7596
                     /locus_tag="ACGI68_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0139013.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7685..8128)
                     /locus_tag="ACGI68_002584"
     CDS             complement(7685..8128)
                     /locus_tag="ACGI68_002584"
                     /inference="COORDINATES: protein motif:HMM:NF025547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HFH0139014.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8152..8946)
                     /locus_tag="ACGI68_002585"
     CDS             complement(8152..8946)
                     /locus_tag="ACGI68_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HFH0139015.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(9511..9987)
                     /locus_tag="ACGI68_002586"
     CDS             complement(9511..9987)
                     /locus_tag="ACGI68_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFH0139016.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSIPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(10088..11257)
                     /locus_tag="ACGI68_002587"
     CDS             complement(10088..11257)
                     /locus_tag="ACGI68_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0139017.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLENPNVKYI
                     FGLHVNSNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            11420..12283
                     /locus_tag="ACGI68_002588"
     CDS             11420..12283
                     /locus_tag="ACGI68_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFH0139018.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWDKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            12354..12947
                     /locus_tag="ACGI68_002589"
     CDS             12354..12947
                     /locus_tag="ACGI68_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HFH0139019.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13198..13833)
                     /locus_tag="ACGI68_002590"
     CDS             complement(13198..13833)
                     /locus_tag="ACGI68_002590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFH0139020.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(13913..14644)
                     /locus_tag="ACGI68_002591"
     CDS             complement(13913..14644)
                     /locus_tag="ACGI68_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFH0139021.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LTLNIELKTDKINYPNIEKGVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            14975..17164
                     /locus_tag="ACGI68_002592"
     CDS             14975..17164
                     /locus_tag="ACGI68_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HFH0139022.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEVPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKFGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDIYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         6630 a         1983 c         2834 g         5843 t
ORIGIN      
        1 gtataaaatt ttaaaaattg aattataact tcaaaaaata aggagggtaa tatgaagata
       61 acagatatca aatttgaaaa gcttagaatt aaattaaaaa aacctgtagt agtttcattt
      121 ggagtaattg aatatggaga aagtataatt ttaaaaattg aaactgatga agggtattgt
      181 ggatttggcg aagcagcgcc tcttgcagca gttacaggtg aagttttgga taatgttctt
      241 tcagtattgc ttatgtttaa aaaagagtta ataggcaaag acccactaga tattgaaacc
      301 atacatacaa tcatggatgg aattattata ggtaatactt ctgcaaaagc agctattgac
      361 atagctttat atgatattaa aggaaaaatt atgaatgcac ccctttataa agttcttgga
      421 ggatttgata gtaaagtcca aacggatatt actataagta tagataagcc agaaaaaatg
      481 gcagtagaag ctttagaaag agtaaaagaa ggttttagga tattgaagct taaagcaggt
      541 ataaatcctg aagacgatat tgaagctgta aagttaatta gagaagccgt tggagagagt
      601 ataagaatta gaattgatgc aaatcaagga tggaacgtaa atagcagtat aaatactatc
      661 aaaaagttag aagaatttaa tgttgatgca attgaacaag cacttccaca ttgggattta
      721 gatggtactg cttacataag aaataaaagt aatacaaaaa ttatgataga tgaatcatta
      781 cattcaccaa tagatgcaat taaggctata aaaaagaatg cagttgatac atttaatatt
      841 aagctgatga aatcaagtgg aatatatcct gctataaaaa tcaacaacat agctgaggca
      901 agtggagtaa attgtatgtt aggatgtatg ctggaaacga gaattggtat aacagcagca
      961 gcaaatttaa tagcatctaa aaagaacata acagaagctg accttgatag ttttatgttt
     1021 tgtgaagaat tagaaggtat atctggagga tttgtaatgg atggagatat tatgaatctt
     1081 gtcaataaac caggtttagg aatagaagta aacttatgat aaatttggag gttaaatatg
     1141 aatacacttt tttcaaatcc aggtagtata cttgctgtaa tgacatcaat gattgcattg
     1201 ggtttttact tacaaagata caaagccatt aaaagcttag gaccagcact tacaataatc
     1261 ataatgggaa taatactttc aaacttaaaa gtagttccag taagtactga gttatatgga
     1321 actatttcaa catatgctat tccagtttct atgactataa tgttgatgag tgttgactta
     1381 aaagaaatga caaaattatc aagagaacct ttaattgcaa tttttgtagc tgtattaact
     1441 gtaagtatca tggcattttt atttggatta gtatttgcag aaaaaatatc agaaggttgg
     1501 aaggttgctg gaatgtttgt aggtacatat actggtggaa gtgctaatct tacagctatt
     1561 ggaacaggtc ttaatgtaag tagacaaact cttgctgcag caaatgcagc agactatgta
     1621 attggagtac caacattgat atttatgttt gctctaccag caatattaaa aaattcaaag
     1681 aagtttaaaa aactttggcc atatcatgta gaagaatcag aattagagga ttgtcaaaat
     1741 gaagaattta tggagtcaaa agaatggagt attaaagata tagcttggat gttggctata
     1801 ggttttgttg ttacagaagt tgcaacaata cttgctggat atttcaattc gagttttagt
     1861 agtgcagcaa gaatactttt agttacaaca atttcaatta taattgctca attaaagcca
     1921 gtaaagaaac taaagggaaa tttagattta ggattatttg ttgcattatt ctttttatgt
     1981 acaataggtt tttcagtaga tataaaagaa tttttaggtt caacttttac tataacactt
     2041 tactgtttta gcataatttt tgcttcattt gttttccatt taggcataac tagactttta
     2101 aagataaagt atcaatatgt aatactttct atagtaggag caattgctga tggtcctact
     2161 tcagctttag tagctgcttc tgcaaagtgg aattcgcttg taagtgtagc agtcgtaatg
     2221 ggagtaattg gtggagtatt aggaaattat gcaggtatat cagtggcata tgcaataaag
     2281 atgtctctag gattataggg gggagtttaa ttgataaaat ttaaattata catatctgga
     2341 ctatacagtg gaaatgtaat ctttgatgga gatttattaa ttgaaaagtt gaatcctttt
     2401 acaaataaaa tagaaagttt aaaaccaata agcaaggaag aaaatacata ctatttaaat
     2461 cttacaaaaa tagatttaaa gtctttattt aataattttg atgtatatac taaaagctta
     2521 gtaaacacag ataaagacac agttataaat aacttaggag aaacgcattc taaacttaat
     2581 gaatatatat gggttcaaag aaacaaaaag ttcccactag atataattat tgtagataat
     2641 aaaatagtag gttttatctg tttatccaga gaaacttgta ctatattaat tatggatgga
     2701 tatgaagagt atactgtatt aaaagaatgg gaaaaaactc ataaaaatga agagatatat
     2761 tctataagat ttggtggaaa ttatatgatt gatatgaaag atggtataaa actttctaca
     2821 gatgtatatc tccctgattt tgtagattct accaaaaaag caccgacaat tcttatgaga
     2881 actccatatg gaaaagaaaa tgataaagaa atatattata aatatgtaca aagaggatat
     2941 gctgttgtaa tacaagatgt taggggaaga aatgaatcag aaggtaagtg ggaacctatg
     3001 attcatgaga gggaagatgg agatagtacc ataaattgga tagtatcaca ggaatggtca
     3061 agtggaatag ttggaatgct tggagcatca tatcttggtt atgtccaatg ggcagctgca
     3121 tcttcaggca ataagcatct taaagcttta gttagtatag taacatcagg aagccctttt
     3181 attgatatac caagaaaagg aggagctttt gtatctggta tgcttgcatg ggcatttatg
     3241 gtatcaagaa ataaagttga tagaagcaaa atggtaagag atgattggga tgatgtttta
     3301 aacatacgac caatagaaaa tatacctgtt gaagctcttg gatatagaat tgaatttctg
     3361 gaagaatggt taaagagagt agaaaaagat gagtattggg atttgatgga ttggcattta
     3421 caaaaagata aaataaatgt acctgcattg gttgtatctg ggtggtatga tgataatagt
     3481 atgggaacta cagaggcctt agatgttata aaggactatg aaaaaggaaa gagaaaggct
     3541 attctaggtc catggatgca taattcaaat actttaagag atataaatgg tatttcttta
     3601 ggaaatagtt cattaagata tgatttggat tataattatc tattatggtt tgataaatac
     3661 ttaaaaggta tagaaaataa catagatacg actgctccag tagagtatta ctctgtaggt
     3721 tctaataagt ggaagacaga agaaaattgg ccaataataa ataagataga taaaagtatg
     3781 tatctaataa gtgatggaaa tgctaacaca tctttaggaa atggtagatt agtatttgat
     3841 aatggtttag aagaaaagta tgatagttat atatataatc caaaagaccc atcagtacaa
     3901 ctaattgata tgtcagagaa tgaagttgga gttccaaata actacaaaga tttagaaaaa
     3961 agaagtgata tgttgtgtta tacatcagat gcttttagtg aagaatttac tgtaacaggt
     4021 gatataaagt tagagttttt tgcatcaagt tctgccaaag atactgactg ggttataaaa
     4081 attatggacg tggatttaga tggaaattca attaaattag ctgatggtat acttagtgct
     4141 agatttagaa atagttttta taagtcagag tttatggaag aaggagagat ttataagttt
     4201 acgattataa cttcaaaaat atcaaacacg tttaaagttg gtcataaaat tagactagat
     4261 ataacgtcta gtgctaaaaa ctttattttc caaaatagca acacgacaga gggatataat
     4321 agtattgaat atatagaagc aaagaatacg atttatcatg gaggtaaata tccatctaaa
     4381 ttgatattgc caatagagaa taagtaaatt tatatagatt aaatagtgag gaggtgtctc
     4441 aaaatgaatt ttttagttcg tgagacactt tcttttatac aaaatcaaat gtattttcat
     4501 catttcaaca atgaaaaaga ggcttatctc tctgttttga gatagcctcc tataagctaa
     4561 attgattatt ttgtagctgc aacaacgctc atttcaacta acaaatgctc tctagcaagt
     4621 ctagcttcaa cacaagctct agcaggttca aatccttttt caacccaagc atcccaaaca
     4681 gagttcatag cttcaaagtc tttcatatct cttaaataaa tagtaacaga tagcaaatgt
     4741 tgcttatctg aaccatattt gtttaataaa tcttctattt tagctaaaac ttcagttgtt
     4801 tgttcaatta ctcctccttc agcatgagtt tgtccacata aataaactgt attgttatga
     4861 actacagctc tactcattct tccagttcct tcatatctct ttatttccat attttaaata
     4921 cctccatagt taaaaaatct attaaaaatc taaataataa attaaaaaaa gtctatacta
     4981 tataaaatta tattatatat tctgataata atccataaaa acatataact ttttagctat
     5041 aattattatt gttattaatt gaaaagattt attagttact tgacatagtt tacactttta
     5101 attatagtat aaattggaat atttagataa ttattattta atgtataagc taagttttta
     5161 attaatatgg ttttatacat ttatatagaa aagaggtacg tatgagtagc acaatcaaat
     5221 caaatcgaat aaaaggtata atgtttataa tagcatcagc gcttggcttt gcaatgatgt
     5281 ctgcattcgt aaagctagct ggagatttac caagttttca aaaagtgttc tttagaaatc
     5341 tggtatcagc tataatagct ttttggctta tcataaaaca taaaggaagt ctaactggta
     5401 aaaaagaaaa tttaaaagta cttttatata ggtcaatatt tggaactcta ggagttatat
     5461 ttaactacta tgcaattgac aagttgatgc tttcagatgc taatatgcta aataaaatga
     5521 gtcccttcct tgtagtaata ttttgtgcaa ttgctctaaa agaaaaaata aatgtaaagc
     5581 agataggagc tattataatt gcatttatag gagctttatt cataataaaa ccaacattta
     5641 gtgtagaaat aattccatat cttgggggaa ttgcaggagc tgtatttgca gcaatggcat
     5701 atacttgttt aagagttctt ggagatagag aagattacta tacaattgta tttttctttt
     5761 cagttttttc attagttact gtagggccaa ttgcttttgc agtatatgaa ccaatgaccc
     5821 ttatgcaact tatatatcta ttactagggg gagtatttgc aagtctaggt caatttggaa
     5881 taactctagc atataaatat gcacctgcta aggaaatctc tatatttgac tatagtaata
     5941 taatcttttc agcaatttta agtatattct tattcaatgt ctatccagat atgttaagtg
     6001 ttgttggata tttaatagta ttttcggcag cattttatat gtttttatac aataagaagt
     6061 tagataaatt agacaaagaa aagaagaagt aaactagata aaagtgtttt tataatttgt
     6121 attgaatatt taagtctttg tattaaaatt aaagaaaatt taattaaaaa tgtttggtat
     6181 attaacttat aacagcagtt aaaattgaaa aattataact gctgtttctt ttattaaaaa
     6241 tatccaaatg tctgtaaaaa tagactataa tagagtagta taaataatat aaaggggata
     6301 aattaaagta tggaaaaagt gaaagttgct gtgattggag caggtaatag aggaacgtat
     6361 gcatatgctc catatattta tgaaaattct gatgtatgtg aaatagttgc tgtagcagaa
     6421 cctaaaaaag gaagacgaga actatttaca caaaaatata acctagatag taaaaatgta
     6481 tttgaaactt tagaagactt ttttaaacat gacaagatgg cagatgcagt aataatagct
     6541 acaaatgatg atagacatta tgatgtagca aaactagctc ttgaaaaagg ttatcatgta
     6601 ctattagaaa agccaatgtc taatagttta gatggtctgg tacatataga tgacttgtgt
     6661 gataagtata aagataagat ttttatgatt tgccatgtac tgagatattc tccattttat
     6721 aataaattaa aagaaattgt agaaagcaaa aaattaggag agctagttag tatacaatat
     6781 aatgaaaata taggatattg gcattttgca catagcttta ctagagggaa ttggagaaat
     6841 agcaatgaaa ccagcccttt aattttagca aagagttgtc atgacatgga tattttgtta
     6901 tatttagtag gaagtagatg caaaaaaata tctgcatttg gaagcttaaa gcatcttaat
     6961 aaccaaaatg ctagtggaga gatggcacaa aattgtcttc agtgtttagt tgaaaaaaaa
     7021 tgtccatatt cagcaaagag aatttattta gaaaaagata gaagtataaa tagagctgtt
     7081 cacataaatc caactgaaga gaatctatta aatatactta agacaagtcc atatggaaga
     7141 tgtgtttaca gatgtgataa taatgttgta gacaatatgg taaatatact acagtttgaa
     7201 aatggagtga cggctacttt taatttatgt gcatttacta aagaaaatgg aagaactata
     7261 aagttaatgt ttagccatgg tgaagttggt ggggatttaa ataaaaatga gataagaatt
     7321 aaagaatttg gaaaaaacga agaaatagtt atgaatccat caaatcaaca gaatatggta
     7381 gaggaatatg atagaaatct tatagctgaa tttattaaat tagtttcaaa taaagaacta
     7441 gaaaagggta gagtagcagc aaaagaagct atacaaagtc atgtaatggc atttgcagca
     7501 gaatattcta gggtcagtga tgaagtagtg tatatagaag aattttttga tagtgctaag
     7561 caaatgacta aggagataga agaaactata ttttaattaa taaaaattta ttaaataaaa
     7621 taaagataga ttaaataata gaaatacgaa aatatgtgtt ctattaagga atctatcttt
     7681 tttattaagg ttttttctta tccctatcaa aaaaatccat acattctgat aaaagataag
     7741 aaagtaaagc gaataagatt tctgtactta aaggaatcgt tactgaatcc ataaaggtat
     7801 caattatgtt catagctaga aaagttagta taaaatcaat tataaattcg caaagtttat
     7861 atattgagta tggtaatttg tttacatatc tgaatatatc taaaatagta gtgcatagaa
     7921 aatcaattgg agtggttata caaaaatata ttaaaaagaa aattaataca gctccaagag
     7981 atttatattc aaatcctaag aatgataaga agccaattcc tataaccgtg aaaatacaaa
     8041 aagttatacc aaaaaaaaca gcaaaaaata aaaatacaga aataaattcg cctaaaggaa
     8101 tccttctttt attacttatc ttaatcaact ccttaattta tgatatgaaa tttatataaa
     8161 taataacatt cttaaaaatt cataatagtc ctctgtttca aatagtactg tttgagaatc
     8221 tatttttttc atatttggat aaaaagaggc cttaaaagct ttataatgtt ctctaaattt
     8281 tatttctatc ttaaatttat ctggcaattt tattaagtgc ctagataaat ctccagataa
     8341 agcttttttg acaccttttt caattttttt tagtgcaagg tttggatgga ttgaaattga
     8401 accatttcca attccttttg acacaggaac agtgactata tttggattta gtttttttgc
     8461 actctcacaa agcatttcat ctccacttaa aaatgctaca ggaacattat aatatgcagc
     8521 aatataagca tttatagtaa attcagatgc tatttcatca tttattttaa aatagtcata
     8581 atcaatatcc atagtatggg aaagtggatt accattacaa gtagctccag agtgatagcc
     8641 ggtgaatata catgcatcaa agctttcatc aattccagcc atcataacta gtgggtcttt
     8701 ggtccatcct ctaagtattt tgacgttttc aggtaaaata tttggattaa tatttctacc
     8761 actgtcatgt gcatctttaa caagtatttc atgtacacca gattgagtag ctccatcaca
     8821 tgctgctgtt atctctttac tcatttgtgt tctatagtgt tgcgaaaatt cacattcaag
     8881 ttttgtttca tcccaattta caataccaca agtaccttct atatcagcac ttaaatacaa
     8941 tttcataata tataccccct tatgtttatt attgatgata ataacagata acagaaaata
     9001 cataatcaat aaagggcata aaaatattat gtcttactat taattattat taaatctgta
     9061 actgaaaaat aactaaattg gtattaaaaa atataacact atgtgtcatt tatttatata
     9121 gtttttatat ctgatgttta tataaaattg taacatataa gtggttacat ttactagttt
     9181 aacatagtta aaatagttag gtattatttt taagttaaat tgattattat aaaatcgcag
     9241 aataaaagaa agatttaatt tattaaaatg catgaaatgt tttttcctca acataatgct
     9301 aaattaaact caaaaagaaa taatagaaaa atcttatgta gcttagaaag aatgtgaatg
     9361 atttaaggga aaaatagaaa tttattatat aatattaggc tgtattatat aatacagcct
     9421 aatattatat gtatatgtga cataaaatct tggtaagtgt aaataaaata aatgtggaaa
     9481 acaagttaat gaaatcaaga aaaaagtaat ttaaaattca atttcatctt cccaattaaa
     9541 ttctcctgat ttttctacac tcttaggcca gtgactacac catttaggaa tggacggatt
     9601 ttcaacccta tcaacgcttg gagtatatgg ctttaagtct ataacaggac tattatcatt
     9661 agcatcaata tatggtattt ttattatacc attttcatgg tcgatattga taatttgtac
     9721 agcagtcaat gctattggat tgggtcttat aggtgacctt gttgcaaata ttcctataac
     9781 gtctggtcca cttttataag gttttggggt ttctaaaata cttcttagtt caggattatc
     9841 aaacccatca gcccaccaaa atacatttaa atgactgaaa tcgtcgagtt catttaatgc
     9901 aggtacatac tttttgtcta atttaataaa gacctcttcg ccttgaatac atattttacc
     9961 tataggattt attaatattt ctttcataat attacctcca attatttaat ttatcatttg
    10021 taattttagg gtataacatg acatcatgtg agggtcaata taaaataata aataaaaata
    10081 ttaatagtta gaatgataat acatttttta catggagcat gacaccagta ataaggcaat
    10141 cttcgtcaat atcaaaagta gaggtatgca aaggggaagt aatgtttttt tcacgatttg
    10201 cacaaccaag gtggtaaaaa gctccttcac agtgtcttag aaaatacgaa aagtcttcag
    10261 ccccaagagt gggcgagtct ttaaggataa tattttcttc tccaaacaat tctacagcat
    10321 tttgtttgac gtaatctact agatggtttg aattgataag agctggataa ccttcctcaa
    10381 tttcaacaga aatacttcct ccaaatgttt tacaagtaaa atcgcaaatt tctctaattc
    10441 gtttttttga aaactctcta gtttcaggag tcaaggttct caaagtaccc cttataacca
    10501 ctttatcaca aactatattt tccttgattc caccttcaat ctttcctaga gaaataacca
    10561 cagaatcagt tggatttgtg tttcgactaa cgattgtctg tagagctgta ataatatgag
    10621 cagatataac aatagcatca ataccttgat gtggataagc tccatgacat tttgaaccat
    10681 gtacagttaa ttgaagtgta tcagtggaag catttagagt gttgtatttt aattcaatta
    10741 gatttttgtt gatattggaa tttacatgaa gtccaaatat atattttaca tttgggtttt
    10801 caagacatcc atcttctatc ataagttgag ccccaccaat agtttcctcg gcaggttgaa
    10861 aaaagaattt tacatttaca tttaagtaat cctttaattt aaatagtata ttacaagtac
    10921 ccagtaaaat agcagtgtgt gcatcatgac cacatgcatg cattttgcca atattaatag
    10981 atttataaga ataattaagt tcttctgtta taggtaaggc atctatatca gctcttatag
    11041 caactgtttc aaagtttgga ctcacgttaa tgtatgcagt tatccctgta tgattcttat
    11101 attcaatata atttatacct atttcattta agtatttaat aatttttttc ttagtttgaa
    11161 attcttctaa tccaagttct ggagtcttat gtaaatctct tcttatattt ataacccaat
    11221 catttatctc atgacaaaga gtttttatat taagcataaa acacatccta actatattaa
    11281 aaatttattt ttcttcatta attatattat tttatgtaaa tcattattat gtatcaagta
    11341 caaataaaat atcttataat tatattagag atatttagta tggagaagaa tatatcattt
    11401 tataagaaga ggtgtttaaa tgttaggaat aattgcgcca tctgggccat taagaaatac
    11461 aagtttggag gaaataaagt ttaatttaga aagttatggt tatgaagtta agttttctga
    11521 aagttgtagt ttaaattata aaggttattt agctggtaat gatgatatta gagcaaggga
    11581 tatagaagat atgtttttag ataaagatgt agatataatt atgtgcttaa gaggaggtta
    11641 tggaacaaca agaatattgg ataaaataaa ttatgatata ataaagcaaa atccaaaacc
    11701 atttataggt ttttctgata taacaggttt aaatttagct ttctataaaa attgtggtct
    11761 tttaccatat catggaataa tggcagcaaa tgtaggtaaa tgggataaat ttacctataa
    11821 atcattggta aatgctttag aatttaaaga tgaattatat ttagaaaatc caaaagaaga
    11881 aaagatttat actgtatgtg aaggtaaagc agagggaata ataatgggag gaaatttatc
    11941 cttaataatt gccacaatgg gaacaaaata tgaaatagat gcaaaagata aaattttgtt
    12001 tattgaggaa attggagaac ctcaatataa actggataga atgttaacac aactatattc
    12061 ttcaggaaaa cttgaggaat gtaatggtat aatttttgga gattttaagg attgtattga
    12121 agaaaatgat ttgatggaat tactaataga atttgcaaat aaggtcaata aaccttctat
    12181 atataatctt cagtcaggac attgtatccc aatgataact attcctttgg gcagaatgtg
    12241 cgaattagat gctacagaaa aaatcgttaa attaaaaaaa tagtgtgtat ataaatataa
    12301 tagaaatatg gtataatctt atacaagtaa aaatttagct aggaggatga aaaatgtctg
    12361 atgatagaat aatagacttt aacgaactaa agaacaaagt taaagattct gatgtagata
    12421 aatttgagca atacatatat aacttatatt tttctgttat ggatggaaag atgtctatgg
    12481 ctgagttttc aagaaaaata ttcgattata tgagagataa caacatatct caagaaaaat
    12541 ttatgaaaat acaaaagcaa tttatggaga gatatggtat ggacactgaa gaagtagaaa
    12601 aacaacttag aaactttgga atcgaccctt ctacggcagg ttttatgagt aataatactt
    12661 ccagcaaagt atctacagaa gatttagagt catttaagaa gagtgcagga ttttatgaga
    12721 aatatggaga aaaaatccaa ccaaagagtt gtataacaac atttataaaa aatgacttaa
    12781 atgatattaa tgtaataatc gaccaagaaa agataatgct ttgtagtgat agaaaaataa
    12841 atcttatgga ttcagaacta aatgagtttt tattagaata taagaatatg tttaataaaa
    12901 aaataaaggt agtaatgtgc gaaactacga ataagtatga ttattaaaaa atgaatttaa
    12961 atactgaagt agtatcaatt aattgtacta cttaaaaata aaaatcgcaa tttattattc
    13021 cttataaatt tgtataagga atatctaaac tgcgattttt tatatagtga agattagtaa
    13081 taatagatag tgttttaaat tgtaatcttc tcactaaaac tgtgtttttc tattagaaat
    13141 tttattaaaa ataaatatct gataattgta ttatatggga tgtaaagtat tagaagctta
    13201 acgaatctct tttaatacat taactccatt ttgaatcata ctgttgaaaa atactaagtt
    13261 taaaatctca gcagggtgaa cgttaggaat atcataagta tctactaaat tcataggtac
    13321 aactatattt ttttcaatat tatgttgatt aaaatatgat tttagagtta ttgcaaattg
    13381 atatatacat atatctgtgc aatcgccaac tataattata ttatctatat tatcaaaatc
    13441 caaattttct aatgcaaaaa aaccattagt agaattttta ggaagaattt gtaggttttc
    13501 tatcgactta agttcatcaa ccaattcact ttctacatca ttttccaaac agtgaacagg
    13561 ataactcaaa agttctattg atttaggagt atgagaatca gtaaatgcta ttactctatt
    13621 taatttgttt gatatttttt ttgtaaacat ttcaattggc tttattaaag actctactct
    13681 aggagagtat aatgcacctt gtcttgcaaa gccattatta acatcaatta taaataaagc
    13741 agttttactc aaatcataat tttctatttt ttctattggt agattatcta aattactctt
    13801 taaagcttca agttcattta ataagttatc catagttata cctccaagat aaatttaata
    13861 aaattgtaca tattaattat ataaaagtca aggagtacaa actattgtat aattagctgt
    13921 attgttttga gtgtaaaaga tttgaagcaa tatctggata gttagtaaaa ataccatcta
    13981 cattgagttt tagacagtgt agtatatcat cctcttcatt tacagtatat acattgacac
    14041 caagtagatt tttgtgacaa agttcaatta gagattcatt tactaaaact agaggtggat
    14101 gaagataact attacccaga aatttagcaa aattgactac attgtttata ggtaaataac
    14161 acaataatcc tgtttttaca ctagggtcta atttatgaaa gttttttaag ctattgctgt
    14221 tgaaactaga tataagaact ctatttttca tattatactt taaaataagc tctaaaacgc
    14281 ctttttctat attgggatag tttattttgt cagtttttag ttcgatattt aaagtaaggt
    14341 ctgtaggtga gattagaatt aatacttctt ctaaagttgg aattttacat tctttattgc
    14401 tttgatatcc ttcaaaagat gagtttagag tttttaattt tcttaaagta aagtctttta
    14461 caaaaccatg tccattaaaa gttctatcaa ccttttcatc atgaataact acaatttttc
    14521 catccttagt tctgtggaca tcaagttcta ttccatatat atccatatcc aaacattttt
    14581 taaaagctag aatagtattt tctggatact ttccactaaa ccccctatga gcataaatat
    14641 tcatttatat ggttccctcc tataagtata acttatctta taaaaattta attaatataa
    14701 tatatcatat tccaaaaggt attgttatat attagttaaa taactatttg taatttatat
    14761 attatataaa tgtagaaaaa taaaatcata tatgtgttat aatatatttt aaacattgta
    14821 aatattacga atttagtagt cataataagt gtaattttat gcaagctcat gtttttagag
    14881 cttgcatatg tattattaat atatttatat attgtgaaaa taaaattcca aaagaatttt
    14941 ttctatagac ttagtagtaa gggggaattt tgatatgtat gaagttaatt tagaacgagc
    15001 tatgaattat tggatggatt tgttagatga ttatacgaat atagcaaaaa ttccaaatga
    15061 ttttttttat aaagattgtt ctgatgaagt agtagatgag gtttttaaga tgaatattga
    15121 gaaacatgaa aggttaattc aatattctaa agtgaacaat attagcatag actctatcat
    15181 agaaactata tatggcctta tacttcaaaa gtatatatat gaagatgatg tagtttttgg
    15241 taaaacaatg aatataattc caataagagt aagagttaga caaagtgaaa aatttataga
    15301 tgttgtaagg aagctaaaaa ggcagtggag caggtcttta gagtatggat actatttgtt
    15361 atcagatata gagaataaaa atcatctaaa tactaagctt ataaacacaa tatttatagc
    15421 taaaagtttt gatttatcat atgattatga catagaaaaa aaaggatatg atttatctgt
    15481 aatagtatat actaagaggg cacttaaaat aaaaataaag tataaaccat ctatatataa
    15541 aagagaaact attagaagaa ttttaaatca atttgattat ataatagagc aaataataga
    15601 agataatgat attttaatag aagatttaga atttgtatgt aaggaagaag aaaaagtatt
    15661 attggaagac tttaataata atcaatatga agtaccctat gataaaagtt atgttgattt
    15721 atttagggag caagttaagg taacacctag taatatagct gctagggatg aaaatagaaa
    15781 gttaacatat aaagaattgg atgaagtaac tgataaactt gcaggttatt taaatcatat
    15841 aggagtggaa agtgaagaca tagtagctgt aatgttacca agagatatta acataattat
    15901 tacagcaata gggattatga aatcaggggg agcatttttt ccaatagaca caagtaatcc
    15961 agaagaaaga ttgaattacc ttcttgaaga tagtaatgcg aaagtagtga taactacaga
    16021 tgaactcaaa agtaaggttg taaatgagaa taccatagta ctcgatataa atgatgaaga
    16081 aatgtttaag tttggatatg aattaactga aaaaataaca tcatcaaatt gtgcatatac
    16141 gatatctact tcagggtcaa ctggtaggcc aaagaccata gctatagaac ataagagttt
    16201 agtaaatatg tgttattata gcgtaaaatc tataagcgct acagaaaatg atatatgtgg
    16261 tatatactta agtttcagtt ttgatgctgt aatgaaacaa ctattccctt atttgcttgt
    16321 aggtgcatca atagatatta tgcctgaaga agctaaattt gatgaatata cagtaaatga
    16381 atactgtgag gaaaatgaca taactatact agcattgcca acagcatttg caaggttatt
    16441 tatacaaaat tgcaataata attcacttag agttgtacag actggaggag aaagattaaa
    16501 aggatataga aagcgtaatt atgaactata taatgagtat ggaccaaccg aatttactgt
    16561 tgtatcgact tcttttcatg tggacagaga atatggaaaa ataccaatag gaaaacctat
    16621 atttaatact tatgcatatg tattagataa aaagaacaaa ctatgtccaa ttggagcacc
    16681 aggagagcta tgtctatcag gcattcaaat atcaagagga tatttaaata aaaaagggct
    16741 aacagagcaa gtatttgtag agaatcctta caaaacttgt gaacataata agttaatgta
    16801 tagaactggt gatttagtaa gatggttaga tgatggaaat ttagattata ttggaaggat
    16861 ggacaatcaa gtaaaaattg atgagtttag gatagagcta tatgagattg aaaatataat
    16921 aaataatatt acagaaatta aaagtgttgt ttgtatatcg agaacaaatg atgatggaga
    16981 tatttatata tgtgcatatt atgtaattga tgaggaagat agtggtaaga taaatgaaag
    17041 aactattaga gaatatttaa atgagcatct acctccatat atgattccaa caataataat
    17101 gaggatagat aagataccag ttacaccaat cggaaaagta aataaaagag cattaccaga
    17161 ataatttttt tattatgata ggtgtcataa agtgatttca gacatttctt tatgacactt
    17221 atttttataa aataatcaat ttgcataaaa acaagaattt tctgtatata tttactatgg
    17281 tgaaaattta
//
LOCUS       DAYASV010000086        17272 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYASV010000086 DAYASV010000000
VERSION     DAYASV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17272
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.086"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(196..720)
                     /locus_tag="ACGI68_002593"
     CDS             complement(196..720)
                     /locus_tag="ACGI68_002593"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0139023.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(785..1537)
                     /locus_tag="ACGI68_002594"
     CDS             complement(785..1537)
                     /locus_tag="ACGI68_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0139024.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1568..2866)
                     /locus_tag="ACGI68_002595"
     CDS             complement(1568..2866)
                     /locus_tag="ACGI68_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFH0139025.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2883..5147)
                     /locus_tag="ACGI68_002596"
     CDS             complement(2883..5147)
                     /locus_tag="ACGI68_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFH0139026.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5402..6220)
                     /locus_tag="ACGI68_002597"
     CDS             complement(5402..6220)
                     /locus_tag="ACGI68_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0139027.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6213..6911)
                     /gene="cbiQ"
                     /locus_tag="ACGI68_002598"
     CDS             complement(6213..6911)
                     /gene="cbiQ"
                     /locus_tag="ACGI68_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFH0139028.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6914..7198)
                     /locus_tag="ACGI68_002599"
     CDS             complement(6914..7198)
                     /locus_tag="ACGI68_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0139029.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7188..7940)
                     /locus_tag="ACGI68_002600"
     CDS             complement(7188..7940)
                     /locus_tag="ACGI68_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFH0139030.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8122..8314)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8446..10218)
                     /locus_tag="ACGI68_002601"
     CDS             complement(8446..10218)
                     /locus_tag="ACGI68_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFH0139031.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10232..11161)
                     /locus_tag="ACGI68_002602"
     CDS             complement(10232..11161)
                     /locus_tag="ACGI68_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0139032.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11382..11567
                     /locus_tag="ACGI68_002603"
     CDS             11382..11567
                     /locus_tag="ACGI68_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0139033.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11560..12261
                     /locus_tag="ACGI68_002604"
     CDS             11560..12261
                     /locus_tag="ACGI68_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139034.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12263..13186
                     /locus_tag="ACGI68_002605"
     CDS             12263..13186
                     /locus_tag="ACGI68_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139035.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13343..14266
                     /locus_tag="ACGI68_002606"
     CDS             13343..14266
                     /locus_tag="ACGI68_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139036.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14259..14996
                     /locus_tag="ACGI68_002607"
     CDS             14259..14996
                     /locus_tag="ACGI68_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139037.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15007..15780
                     /locus_tag="ACGI68_002608"
     CDS             15007..15780
                     /locus_tag="ACGI68_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139038.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16321..16929)
                     /locus_tag="ACGI68_002609"
     CDS             complement(16321..16929)
                     /locus_tag="ACGI68_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0139039.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17107..17223)
                     /gene="rrf"
                     /locus_tag="ACGI68_002610"
     rRNA            complement(17107..17223)
                     /gene="rrf"
                     /locus_tag="ACGI68_002610"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5564 a         2995 c         2186 g         6527 t
ORIGIN      
        1 tcataataaa atttttttaa taaaacttta ttttttagtt aaaatcatac aatatataga
       61 tttccacaat acaaataaat taaaaagtga aatgtatttg tctaaaaaca taataacatt
      121 tcactttatc ctcattttta agtaatatat atttttaatt tttatttata actaaatcta
      181 gtttttatat aaattttagt ttttttctac atctgagtaa tctattccaa aagtttctac
      241 agtaacagat ttcataactt gtggagtttg tggcttatca gctgaatctg ttctaactga
      301 tgctatttca tcaactgtat ccataccttc tatcactcta ccaaaacctg catactgacc
      361 atctaagtgt ggtgagtttt tatgcattat gaaaaattga gaaccagctg aattaggtgc
      421 catagttcta gccattgata tgactcctct ttcatgtttt aaattgttag taaatccatt
      481 accactaaat tcacctttta ttgagtgacc tggaccaccc attcctgtac catttgggca
      541 tcctccttgt atcataaatc ctggtattac tctatgaaat attattccat tgtaatatcc
      601 ttcatttact aatgatacaa agtttttaac tgtatttgga gctatatttg gatataactc
      661 aattttgatt tcttttccat tttccatttc tatagttact ataggatttt tattttccat
      721 aatttattac cttccttttc attagttaaa ttacataatt acatttatta tattatactc
      781 taaattaaag tctatcaagt atattttttc ctatttcata tccttcttga taaatagttt
      841 tacaaaactc atcatcaaag ttaagcgtgt ctttaggagc aaattgagta ttaggtctaa
      901 ctactattat tttatcttca tcatatactt ttttaatatc ttctggcaac tcataattat
      961 attttgtgga tattaaaata attttatcca cattatcccc aattaatggt tcaattaatg
     1021 cactcctaat taaacctcca tctaatttga aaccatcata aattccttct tgcacatctt
     1081 tcacaaattg ttctttaaat ccaagagttg cgtttggatt tgatggtaac aacccactgt
     1141 atgaaatgct gataatggct tcattacgag gcatttttga tacgtcaaca atcttttctg
     1201 atactacttt attatcaact tcaacataat taacataaaa gttcatttct tctatattac
     1261 tatcatctag ttcccttagg ttgtctatag caggagaatt atccacagta ttatttacta
     1321 tttttacatt tccattctgg atatctttta tatttgtcca cattttctcc aagttatcaa
     1381 cattccctgt gtacaaataa tatccattta ttgctcctat tgatgtccct gaaatcgaat
     1441 aaaatttatt tattcctcca tcatacaagg ctttaactac tcctgcttga taagcccctt
     1501 ttgctcctcc accttctaaa cataatccta atttcatagt ctcatctcca aattgtttat
     1561 cttatattta tatttcaaaa aacttactaa atgttttata tatactatag tggtcctcaa
     1621 catttcctaa ttctcttata gaatgcattg ctaaaattgg acttcccaca tccacagatg
     1681 gtatatctat atgagtagaa gatattggac ctatagtact tcctcctctt tcatctgacc
     1741 tattaacaaa ttcttgatac tcaacattag cttctctaca tatattttta taaatagcta
     1801 tagaatatcc atcactagta tatgcctgac tagcatttat ctttataacc ggaccttttc
     1861 ccattattgg tttattagtt gggtcatgct tttctggaat gtttggatga atagcatgag
     1921 ctaaatctga tgatatcata aaagatgaat aaatagactc aaagaatccc tctctatcct
     1981 tacccattga tatgcaaatt ctctccaata tatttaacaa catgtttgaa tctgcacctt
     2041 gtttagtaga gctaccaact tcttcattgt caaatacagc cattacattt acaccatgtt
     2101 gacctttagt attaacaaga gcattcaaac tagcatgtac cattgcaaga ttatcttgtc
     2161 tcccaataga tataaattca tcattagccc ctattaaact tcctttttca aattcatata
     2221 aaaataaatc aaaatctata atatcgtcca tatttacatc taagttagat gctatttgct
     2281 ttataagaaa attatctttt tctaatgtat catttaacat cccaactaaa ggtaacatat
     2341 ctttttgctt attatatgag tgaccatcat ttatacttct atttaaatgt attgctagat
     2401 ttggtattat acaaattggc tttttaaagt ctataagcct ttcatttggt tttaatatac
     2461 tatcaccttt taaaactact ctaccagcta tcccaagagg tctatccaac catgtactta
     2521 atattgctcc tccatagcat tctgtattta acttcaaata agatttttca gcttctattt
     2581 ctgcatttgg tttaatacta aatgtaggag aatctgaatg agagcctatt attctaaatc
     2641 catctctttc tataccttct gaatttacta caaaagctac tatagctgat aaattcttag
     2701 taacataata ctttccacca actcttagat tccatctttg attcaaaact aattcttcaa
     2761 atccattttt atctaagatt tccttagtac tctcgactgc atgaaaagac gacggactat
     2821 catatatgaa gtctattaag tttttagcaa agtttatact atccattcct catttacctc
     2881 cattataaaa tatttagtgt tgtatattct ttatttattt ttttaattcc ctgttggtca
     2941 aaagctatag taacatctgt accaattata gaaaccactg tcccaacccc aaatttagga
     3001 tgatgtacct tagaaccaag ttttatatca tctggattag tttctttatt agaatctttt
     3061 attgtagcat taactttatc tgcaacttta gatttgctta tagtactcat atacttttta
     3121 gtatatttat ctaacatatt ataacttgct ttagaatagc ttagttcttt ttttactttg
     3181 tacaatcttt ctatacattc ttctggcagt tcttccataa atcttgaagc tattgcaaca
     3241 tttgttttac catataatgt tctcttctct gtcaatgaca tatatagctc ttctttagct
     3301 ctagttatac caacataaca aagtcttctc tcttcttcta tttgtgaatc actcatagat
     3361 tttatagctc ttgatatagg aaataaccct tcttccatac ctgttaaaaa cacaacaggg
     3421 aactctaaac ctttggatgt atgaattgtc ataagcgaaa ccctatcatt gtcttcttca
     3481 tcatttgatt cagatgtaag tgctatactt gttaggaatg tctctaagtc tttattttca
     3541 taagtatcat cattactact ttcaaactcc aaagctatag aaataaattc tctcaaatta
     3601 tcaattctgt cttgagcttc ttcacctttg cctgttaaat ctttttcatt tttatttctt
     3661 atctctacaa gctcgtccat atatcctgta gtatccaaga ctttttctat aagtttgcta
     3721 acaggataaa cttcttttat agttctaaga gtccctataa tatcaacaaa tccacttata
     3781 cttgcccttg ccttagtaga tatatctgaa tttgtttcta tatcaattaa tactgagtat
     3841 atactttcct gttttaagtt agctctatct tcaatttttt ctatagttct aagccctata
     3901 cctcttctag gaacatttat aatccttttt atagatatat catcttgtgg attttgaata
     3961 actcttaaat acgccaacaa atcctttatt tcttttctct catagaattt tgtaccacca
     4021 tatatattgt atggaatttg acttctattt aaagcgtcct ctacaggtct tgcctgtgca
     4081 tttgctctat aaagtactgc aaaatcctta taaggtctat tttcttttct agctatttgt
     4141 gcaattgaat cagcaatgaa atcagcctct tctatttcac taccagttaa ttgtatcttt
     4201 ataagttctc cttctttttt ttcactccaa agcttttttc tttttctttc tatattatta
     4261 gatataactt tattagctgc atctaatata acttgtgtag acctataatt ttgttccaac
     4321 tttacaacat gtacattgtc ataatctttc tcaaattcaa gtatgtttct tatatctgca
     4381 cctctccatc cataaatact ttggtcatca tctccaacaa cacaaatatt ttgatgttct
     4441 cttgctaata attttatcaa ttcatattga gctttacttg tatcttggta ctcatctacc
     4501 attatgtatc taaatctact tctgtaatat gctaacactt catcattagc cttgaaaagt
     4561 tctacagttt taagtattaa atcatcaaag tcaagtgcac tatttctttt taaacggtct
     4621 tgatatagcg cataaatatc tgcaatctta accattctat tatctgccat attgatatct
     4681 ttaaactgct ttggagtata aagtttgtct tttgctcctg aaattgcact tataactgct
     4741 tttggttcaa acactttatc acttaaattt aattctttta gacagtcttt aaccaatgta
     4801 acttggtctg ctgaatcata aattacaaaa cttctattgt aaccaatctt atttatatct
     4861 tttcttagta tacgaacaca gcaagaatgg aatgtactta tccacatatc cttagtttca
     4921 ggacctatat tttgttcaac tctctctctc atttcatttg cagctttgtt tgtaaatgtt
     4981 atagctaata tatttggtgc ttgtacacct ttatcttcta taagatatgc tattcttgtt
     5041 gttaatacct tagttttacc tgaaccagca ccagccagta taagtactgg accttcagtc
     5101 ttttctactg cttctctttg agcaggattt aatgtatcta aattcatatt tctcctcctg
     5161 ttaacagcca taaatatatt ttaatctaaa atatgaatta cttcaaatta taattatgta
     5221 ttatattaat agattatttc caatatatta caattaaata acatttttcc atataattta
     5281 tttcattgtt taataaaaaa aataacctta tgatttgggg aatcataagg cacctttaag
     5341 gggtctaggt taagggttat tatatataaa tctatttttt taaaataggt ttagggctta
     5401 attatttatt tgtattaagt tctctttcct tccaatactt atacaaatct tcttctttct
     5461 taaataaggg taaattcata tttctatgta cttttactaa ccatggggat tctaggccag
     5521 cttgtataat attattttca tttataaaca cattttctgc ttttccttca tcaatcaaaa
     5581 tccccttatc caatacatat atgtaatcac atatttcata cattaaattc atatcatgac
     5641 tggatattac tatttttata ttatttttat ttaatccctt tattatatct acaatagacc
     5701 tagtacttac tgggtctaaa ccagctgtag gttcatcaag taatacaatt tcattttcca
     5761 ttgctataac tgatgctatt gccactcttt ttttctgtcc atagctaaga aaatgtactg
     5821 gcctgtctat aaaatctatt ccattaacag ctactagagc tttgtttatt ctctctttaa
     5881 ttgttttttc atccatacca atgtttctca ttgcaaaggc tatatcatca tatacttttg
     5941 aataaaaaat ttgtttttct gggtcttgga agacaatacc tacatttttt ctaaggttgt
     6001 ataaacttct cttatcatat tttaactttt cttctttaaa aagaatttct ccagatgtag
     6061 gctttaatat acccattaaa ttcataaata atgttgattt tcctgaacca tttgaaccta
     6121 ttatacctat aacatttccc tttgaaaaat ccatattaat gtttaatagt gcattggtat
     6181 ttttttcata ttgatacgtt aaattattta ttttaaacat cattatcccc tactatgtga
     6241 aatttaccat catacaattt tatgtctaat gatatacaca tatcatcata ccttgtcata
     6301 agtcttttgt acagcatact tccaagaata cctaaggatt tatatgaagt ttttaaattt
     6361 atatatccaa atcttagctc ttgtgacttg tatatttctg aaaattcttc taaaaatatg
     6421 aatataaacc tatatattaa cattgataac tcgacaaaag tgtcaggtaa atgaagtttt
     6481 ttaaaaaaga atattaattg attaaaaggt gttgtaagta tacaaaaata cacacaagtc
     6541 aaacaagaca tcgctctaaa taacacatga atagacatgt taatagacgc ctttgatatt
     6601 cctacattaa aactaaaaaa ttcaaaacta tataataaat ctgctttatt aaaagatata
     6661 tttacaagag ttattcctat actaagaaat aaaaagtaca ttggtattct aagtaatttt
     6721 aaataatttt ttaaatctat tccagccaca caaacaacca aaatactcat taaaatcata
     6781 attccactta aaataaatat attttgtatt accatagaag caatcagaaa aattactcct
     6841 atggctactt ttttgtttgg atttacttta ctcaatctat ttgtatatgc atatttatca
     6901 attataagca tgattaactt ctctttcctt tttgaagacc taatatgtat cctattacac
     6961 cagcccctaa agctgcttgt gcagagaata ataaactttc tatttctcca cttggtggct
     7021 catatggact gctaaaccat ggtttgtaat ttggatttat cttagtaatt tcactttcag
     7081 cttgaccatc tgctcctcca tattcagcac ctgagttgac aagtaatggg aatataataa
     7141 gagctactac taataaaagt aataatatgt tagtttttgt ttttgtttta gcactcatta
     7201 gctttaactc ctttatcatt attttctagt atgctttttt ctccgttttc tgtaattaaa
     7261 ttatatacaa ttacagtaag tataccttct gctattgcta ttggtatttg agtcataaag
     7321 aaaattccta aaaatttaat tgctgaagcc attaccccac ctgatgggtc tgggaaagcc
     7381 agtgctaatt gtattgaagt tattgtatat gtggctaagt ctcctattgc tgctgcaaga
     7441 aatactggaa tagcattatt tccatctttt ttctttaaag ccttaaatat taggaatgat
     7501 acgattggcc ctattatcgc cattgaaaaa gcattagctc ctaaagtagt tattcctcca
     7561 tgtgctaata acaatgcttg gaatattaaa actatagttc caagaacaaa cattacgctt
     7621 ggtccaaaca ttattgctcc aagacctaca ccagtaggat gtgaacaact tcctgttact
     7681 gaaggtattt ttagtgctga taatacaaac acaaatgctc cacataaagc taataatact
     7741 ttctttttag ggtcttgctt tactatttta cctattgatt ttaatccgaa taagaaaaat
     7801 ggtataaata taactcccca tgctatagac cattttactg gtaagtaacc ttccattata
     7861 tgcattgcat ttgatgtaac tggagttaaa atgattaaca taagtgcagc tataacccct
     7921 aatttaatat tctgtttcat cttagtatcc ctctctccct ttctaataat taaaacataa
     7981 taatacatgt ctgatttatt taataaaata aaaaagccta actcgatggt taggcttcta
     8041 attttccaca atctaaatac aaacacataa aacaaacata tctgtataat tattataaga
     8101 aactttctcc atccccgaag aaattacttt aatattttag gcaggtctcc tgacttagct
     8161 tcatcctact ccttttcctt cccatgagat ataaaaaata tcatcatagt ggctaacaaa
     8221 agtttcgtct gcatcacagt agcgggggct gtaaaggaat ttaacctttt tccctattaa
     8281 tcttgcttaa agaacctata atattatata tttaattttt aaattattct taaatcattt
     8341 tctatataaa tcattgtata ccttttctgt gaaaatttct acatttttta taaattttat
     8401 tcatttttat atttttatgt atataaattc ttttttgtaa gtttattaat ttctaattat
     8461 cttctttaaa tacattgaaa ctacatcaga aaatctagtt atatctcttg aatatataaa
     8521 atcatttcca tatattaatc tttcagcttc aagttctgat tcttttcctg taaatactcc
     8581 taatactaat atattttgct ttctagcttt tctcacttca ttggctgtat ctctcaaacc
     8641 aactataccc ctataagcct tttctcctct taatgttctc tcactacttt ttcctatctt
     8701 tacatcattt ggtcttccat cgctgagaac tatcaggatt ttattttctt catctctatt
     8761 tattagacca ctacatatag attttattgc caaaccatct ctattattac cagcacaaaa
     8821 atattcaaat atattttcac tattttttat actatcatca taatctttaa atctttttaa
     8881 tattgtatag tctaaaaaac tagaaaatcc catcactcta tttggtatgc caacttctgt
     8941 tagagcattt gcaattatgt aagcttgaat tgccacattt ccctgatttc tactttgaga
     9001 gccacttgca tccaataata tatcaataac gtatttccct ttttcatttt ctatatcttt
     9061 ataaaatatt ttattattat tacttctacc aactctccaa gctctattgg ctaatatagt
     9121 accacaatca gaatatactc tacttttagc attttcttct attaaaattc ttgacagact
     9181 ttcttttaat ctctttattt gctgtctata cattctagca tgttctctaa attttcctaa
     9241 attattttct ttttgtcttg ttacatattt tagttgaaat acattattac actcacttct
     9301 aagaactcca tctgtaaagt gtattctaca accttcatga acatttctac aattttgtgt
     9361 ctcaattttt ttaatttcac ctttactcaa ataagttttt ccataataat gttctatttt
     9421 tgaatatatc ttatttgcag tgtcttcatc aacatatata actcggtctg ctgaattgct
     9481 acatttagaa tcatcaaaat cccctacacc ttctacaagc atggatgaag atattgaatc
     9541 tatatcactt tcaataatat tttcttcttc atcactataa agttcttcat acatgaaatc
     9601 agcaaatgta tcaaaattta cttctacact ttctacatta ttttcatcaa taagactatt
     9661 ttcttctcca tttaatatgt attcaaaata agttaaatat ataacatcta atgcttttaa
     9721 aatctccata atatcttgat tggtatctat atttcttata tcattaacta ttcttctagt
     9781 catactatct aatgctgggt gcttgtccat actataaagt acaaatgtgt atttaatttt
     9841 atccataata ttatcattgt gtattttggt aaaacgtgaa acaatatctt tataagcatt
     9901 ccttcttatg tcttctatcc ctggcctgtc tttaatgacc ttttcttcaa ctattgtatt
     9961 taatataact tgaattagac ctagtataac atctttgttg tatccttttc ttattctatt
    10021 tgtaagatat tttttaacta tatcccaatc tacatacttt cttctagcac ctgcaattat
    10081 tgcgaaatat agacctattt cttttgaaga gtaatctttt tctgatatat ctatactttc
    10141 attgtaatca ccagctattg tccatgatag gttattagct ctattttcaa attcataatc
    10201 atcatatatc catttcataa tctcacctat attaaatatg acttattgga aacacatcta
    10261 tactctccca tttattaggt attctagttt taattacatc tccaaccatt tctttctcat
    10321 atacatcaaa agtctttcct gtaagtccca tattaattgc aagagttggt tttaatcctc
    10381 ttcttatagt cttaagagat gccataagac cacgtaaatc aatagcttta ctagatatct
    10441 ctccattttg agacttcaat tgtaaatcta aaaatattcc tgcaaagtgt attaattttt
    10501 cttcatcagc atctgaaaat tcatttttta aaatcatcat taacttatct tcttctatag
    10561 gaggaatatc tattaccata aatctagata ccagtgcttc attaagttct tttgttcctg
    10621 catattcata attcatagtc ccaataaatc ttgttgctgg atgtagattg actctctcat
    10681 acccaggtac atcaattatt cttctatagt ctaaagctga atgtaccaca actatagcat
    10741 cattttttgc catattaatt tcatcaaaca ccccaaaacc tccattaata gcacactcat
    10801 aaactgagcc tcttctaagt ttaacttcat tgtctataaa tgtatctgta cctataagtg
    10861 ttgaactgtc tgtatttata tgaaatgatg tattccactg aggtctatta aatatttccc
    10921 ctagattatc agcaagtaag ttttttcctg ttgcctttgg tcccgataat aatatatttt
    10981 cttcttctaa aatagctgct atacacatag acaagatatc tttaccataa aaataagttt
    11041 tagactttgt tactctgtat tctaaatctt tttctaaacc ataataattt ctaaaataca
    11101 gtacatcttc aataagtttt tcatctaccc cttgcaccct tagattatct attatatcca
    11161 tatttttacc ccttttatat aaatagtatt tgtttcattt cttatcacca ttttagcata
    11221 aaaggatata agctaaaatt gattttttaa tctccccatt ttaagtaaaa ccatgcacca
    11281 ttttatcact acaaaattaa gagtactact tactgatatt tcttgatttt gttcgtctat
    11341 acgtatataa tatatgctga taaattatga ggaaaggatg attgaaatat ggatgcccag
    11401 aagtagcaaa aaaatggggt atttctgaaa gaagagtaca aaaactatgt gaagaaagtc
    11461 gcataccagg agtttcaaaa ctcgggtata tgtggctgat accaaagaac gcagaaaagc
    11521 ctgttgatgg tagaacaaaa agaagaaagg aagtacaata tgaatagcgt tttgattata
    11581 gatgatgaca aagaactttg tgctcttatg aaaaaatgtg ttgagcaaga aaatttatct
    11641 gctattactg cacattgtgg tgcagaaggt ctacatttgg tcgataaaaa tagacacaca
    11701 ctctctcttg taattttgga cataatgatg ccagatatag acggttttca agttctgaaa
    11761 aaaattagag aaacaagtaa tattccagta attatgctta ctgctaaaaa tgacgaggat
    11821 gataaagttt ccggactacg tttaggtgct gacgattacc taacaaaacc gttcagcata
    11881 aacgaactta tggctcgtgt caattctctt attcgtcggt atactatgtt aaaccctgct
    11941 tttataacag acacagattg cataataata aatggcatga ttattgacaa gacaaaccga
    12001 attgtttctg tcaataatat tcctgttcaa cttacaagca aagaatttga tttacttgct
    12061 tttctggctt ctaataaggg acgggtgttt accaaaaaac agatttatac acaagtatgg
    12121 gaagaagaat atgcttttga cgatagtaac atcatgtctt tcatcagtaa attgagaaaa
    12181 aagattgaac cagaccccaa ccatccattt tatattttga cagttcgtgg tgtcggttat
    12241 cgttttaata aggaggctta acatgaatac aagtgtctat ctgcttttga tattggttct
    12301 tagcctgatt attatagcgt accttgtttc tgtcctgcta cgtgtccgaa tacaacttac
    12361 acttataaaa gatgctttag aggatataaa aaatggaaac cttaatcgac gtattttaac
    12421 agatgaaaac gacattacaa aacaaatttg ctatgatatt aacgaaatag caatcaatag
    12481 ccaatttcaa cttattcagc aaaaacaatc agaacaagcc tacaaacagc taatgacaag
    12541 tctctcccat gatgtaaaaa ctccacttgc ttctttggtc ggttatttgg aagcagtaga
    12601 aagtaaaatt gttgtaggag aggaaaaaga cgagtatatc catgttgcgt ctaataaggc
    12661 tcattactta aagaattttg tagaaaatct atttgaatgg gtaaaattgg attctaagga
    12721 acaagttttt cattttgata tttttgactt gaatgaatta tcccgtaata ttatatctga
    12781 ttggatacct gttttagaaa gcagtcattt tgaatatgaa tttgatatac cagaaataga
    12841 gtacttctta cgtatagacg caaatgcata tacccgtatc attaataacc tattacaaaa
    12901 tattattacc catagtagtg gcaacaaaat gacattgaga atttttgaaa acaaagagca
    12961 agctcaaatt ataataacag ataatgggaa aggtatatct tcagacaatc tcccacatat
    13021 ttttgaaagg ttgtaccaat gcgaccattc acgagcttca aagggaaatg gtttaggctt
    13081 ggcgattgca aaggaactta ttaacgctca taagggaaat attaccgcca acagcactcc
    13141 cggtatggga acagaattta caatattgct tcctaaagcc ctgtaaaagt acgaggcttt
    13201 cttactctaa aacaagataa aagcaagatt atggcaaggt tttggcaagg ttcatgtttt
    13261 ataattctaa ttgtagttga ttttgacgtt gtatcaacta tcttttgtag aaacaaattt
    13321 attaaaagtg aggaaattat atatgaatga ttttgtgatt gaaacaaagc aacttacaaa
    13381 aatttatggg aatcaaacag tagttgacaa agtcaatctc catgcaaaaa aaggccgaat
    13441 atatggtctg ttaggacgca acggagctgg gaaaaccaca attatgaaaa tgattttagg
    13501 attgacttca atcacgtatg gcgaggtaga tgtatttgga gagaatatta agggacgtga
    13561 aaaacaagta tatccccgta tcggtgcaat tatcgaaacg ccaggatttt atcctaatct
    13621 gacaggaacg gagaacttgg agatttttgc aaaactgcgt ggtacagccg cccctaattc
    13681 tgtaaaaaga gcattggaaa ttgtaggctt accttataaa aataaaaagc tgtttagtga
    13741 atattcactt ggcatgaaac agcgacttgg tattgccaac gctatcctgc atgacccaga
    13801 actgttagtt ttagatgagc caaccaatgg tcttgaccct attggtattg cggagatgag
    13861 aaagtttatt agggacttga gcgttgagca tggaaagaca attcttattt ccagtcatat
    13921 tctttcagaa atctcattgt tggctgatga tattggaatt atcgaccacg gtgttctttt
    13981 ggaagaaagt agtatgagtg aactagaaaa gaaaaacagt aaatatatcc tgctacaagt
    14041 ttcagatatt ccgaagacat cacttatttt agagcgtcaa tttaacacga aggattattc
    14101 cgttcaagat gactacacgt tacaccttta taacactaat ctggatatgg gtgaaatcaa
    14161 taaagcactt gtaatgcaga atgtaactgt tattagttct caactttgta atgatacttt
    14221 agaggactac tttaaaaaaa tcacaggagg agagggcatt gcttaaactt attcaaacag
    14281 aatttcttaa actgcgtcgt aggaaactta tttggctgat gttgttgtct gctctcatta
    14341 tgccgttttt cgcactacta tacttcaatt attttggtaa aacaggtgtt gagccaataa
    14401 agttttataa atggtctgca tttggttata cactttggat tattttgcct gttgttttgg
    14461 gtatattttg caccatgctt atacacgaag aaaaccagta tgatatgctc aaacagcttt
    14521 ggattgtacc tattagtaaa atgggatact tttttagtaa atttttcgtt gttttgattt
    14581 actctatttg ttttatgctg attacagccg ttgcttctgt gttatttagt gtactttctg
    14641 gatatgtcgt atttagctgg gcaagcattt tatatttgtt gaaaaaatgt ttagaaattg
    14701 gtgtaatcac tgcttttgta atgttgccga ttttggcaat cgctacatct caaaaaggat
    14761 acattctccc aatttgtatc acattggtat atgccttttc cggttttgtt ataatgacaa
    14821 taaatatgta tttacaccca ttatccagta tgggagttat tatcatgaga aataaagaca
    14881 ttccaggtct tatattttca caggaaatta atgtcccatt agcatttctt tgtatttgta
    14941 tttggagtat tgtatcagta ttgtttgcaa acactgcact aggaagaaga aagtgaggtg
    15001 tagataatgt ggaaattact ttgggctgaa tgtcaaaaaa ttcgtcgttc aaaaattgtt
    15061 tggattacag tttttgcatc cattatgata gcagtcatag tttttgcagg agggcaggat
    15121 gtatataatg acccagattt acattatggc ttaaagagcg tttatgacgg ttcacggtat
    15181 atagacaatg caggttggta tatggacgca gtacaacctt ggtcaacttt ttttgttctt
    15241 cctgctgtca ttgccctatt aggaagctat attatttgtc gtgaagaaga ggaggacact
    15301 attaaatccc tgcgtcttat tcccataaat gaaacaaaat taaccatagc aaaaatgatt
    15361 attactttta tattcagtat acttctttat ttgttactct ttactataac atttttgact
    15421 gaatccgttt tacatttttc cgatttatcg acaaaactgg ttttaagttg catgaaagag
    15481 tattttcttg atggcatagg tgtattcttt gcaatttctc ccattattgc cttggtatcg
    15541 cgcatgaaaa aaggatattg gcttgcatta gtattcacag aagtttattc tattgctgga
    15601 ctatttgcgg gtatgtcaaa tgtcttacaa accttttttc ctattgcggc aatatttaat
    15661 atttctggat actatataac tactggagaa aaaattatgg ggagtatcat aagtttatta
    15721 ctttgtgcct gcctttctgc atttatactt aaagggctaa aacacaatga aaaaaattga
    15781 aataggtata tcaaaatgaa aaagataaaa aaactgatta ttctaattgt attcttgttt
    15841 ctttttggca tgttacactg ttatactgaa tggaaattca acaataaccc acctaaaggg
    15901 gtaatagaaa gaataaatga atggactgat aagattggac tggattagta acttatgtta
    15961 attatagtga atgtaatatt agcaagttat tgtttcgtat tttaatacta taactttaaa
    16021 tatttggttt taatcttcca aacgaatata aaaaaactgt cacagaggca acatattttt
    16081 gaatatttat aaagtggtag ttttgatttt caaagctgcc gccttttctt ctatataagc
    16141 aaacttatcc tcaacaaagt gtaaaacatg gtacatttta agaaaaaagt accacagaca
    16201 caagcaataa aagaaacgcc gagaacctac aaaaacgtag tgcttatgca gatttacgac
    16261 gccttctatt ttctcaaccg tcttaatgac cttgtacaaa agaaattttt ctaatatttt
    16321 ttataatctt atcttgtgat gttttttatg tataaatgac ttaataacaa atccaataaa
    16381 aattatggat attggtataa ttagaacgag gaatttataa tcattataat gtgacattat
    16441 tcttacccaa ctctcagaaa aaatatatcc tatactgatt aatgaagtat tccatatggt
    16501 tattcccacg atagatgata ttacaaaagt tataatattt tgacctgcta tacctgctat
    16561 aaaagatatg tatgttctac ataaagggat taatctacat acacaaacag aaatagaaga
    16621 gtatttgtta aaataatctt gagatgcaaa tattcctttc tttgcctttg gacatatctc
    16681 tattatttta tttatgatag atttacctcc ccaatttccc actgtataac aaaatatcga
    16741 accaagtata cctgcaatta tacttaaaat taaaattgtc agaaatccaa aatgatttct
    16801 tgcagcaatt gctccacata aaggtaaaac aacttcgcta ggtaatggaa aacaagcata
    16861 ctcaatcata attaatacaa atacagagat taatccatat tgtaaaataa aactattgat
    16921 tacagacact tacttatcta tctcctttct tatttaaagg ttttaagggt acatgttcta
    16981 aggattctca ccctatattt aaagaatcaa tctctttaaa ctattatatg tatttacaca
    17041 aagcatttat aacatacttt aaaaatagta ccttaaaata agaaacttaa taaataaaaa
    17101 aagattacgt ggctacgtcc tactctccca ggcagtttcc caccaagtac catcagcgct
    17161 agagagctta acttctgtgt tcggaatggg aacaggtgta tcctctctgc tattgtaacc
    17221 acataatctt tatgcttaat atttctatta agtttttaat aagtttatag aa
//
LOCUS       DAYASV010000087        16833 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYASV010000087 DAYASV010000000
VERSION     DAYASV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.088"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            207..2021
                     /locus_tag="ACGI68_002611"
     CDS             207..2021
                     /locus_tag="ACGI68_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0139040.1"
                     /translation="MIFISFISIKLIHNVGDYGKLINYVGIVRGASQRLTKLEMNHQP
                     NDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKWEDLNIEIKKVRMKEQ
                     NNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISIIGILACIILILQYSKK
                     MVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDKKFAVFYIDFENFKYI
                     NDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRCYVDKEDLVVRQRDVD
                     NELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANFAQKTVKNKPGTNYAF
                     YNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKINCAEALVRWLTPDKG
                     IISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFVQISVNVSRIQFYNTK
                     FVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLHENGFTCSLDDFGKGY
                     SSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKELNITTVAEGIECKEQ
                     VEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2313..2912)
                     /locus_tag="ACGI68_002612"
     CDS             complement(2313..2912)
                     /locus_tag="ACGI68_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0139041.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3015..3812)
                     /locus_tag="ACGI68_002613"
     CDS             complement(3015..3812)
                     /locus_tag="ACGI68_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0139042.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3925..4030)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4122..5108)
                     /locus_tag="ACGI68_002614"
     CDS             complement(4122..5108)
                     /locus_tag="ACGI68_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFH0139043.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     regulatory      5272..5448
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5592..7322
                     /locus_tag="ACGI68_002615"
     CDS             5592..7322
                     /locus_tag="ACGI68_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFH0139044.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLSIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7432..7512
                     /locus_tag="ACGI68_002616"
     tRNA            7432..7512
                     /locus_tag="ACGI68_002616"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7466..7468,aa:Leu,seq:tag)
     gene            7847..8362
                     /locus_tag="ACGI68_002617"
     CDS             7847..8362
                     /locus_tag="ACGI68_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HFH0139045.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8854..8958
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9168..11642
                     /gene="gcvPA"
                     /locus_tag="ACGI68_002618"
     CDS             9168..11642
                     /gene="gcvPA"
                     /locus_tag="ACGI68_002618"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HFH0139046.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11642..13099
                     /gene="gcvPB"
                     /locus_tag="ACGI68_002619"
     CDS             11642..13099
                     /gene="gcvPB"
                     /locus_tag="ACGI68_002619"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HFH0139047.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
     gene            13522..15879
                     /locus_tag="ACGI68_002620"
     CDS             13522..15879
                     /locus_tag="ACGI68_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFH0139048.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(15965..16282)
                     /locus_tag="ACGI68_002621"
     CDS             complement(15965..16282)
                     /locus_tag="ACGI68_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFH0139049.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         6370 a         1945 c         2741 g         5777 t
ORIGIN      
        1 tttgtatata taaaggactc taaagatgaa ctatgtaaaa caaggtttat atattctaat
       61 tgtatagaaa aatttattgt aaacgcatgt atattattag aaaaaatagt ttggaggtat
      121 tcaataaaca taaaaaacaa tataggggat aaaggtgttt tatgaagaga tttttgagga
      181 gaattatctt agttttattt attttgttga tttttataag ttttatttct attaaattaa
      241 ttcataatgt tggagattat ggaaaattga ttaattatgt tgggattgtt cgtggagcta
      301 gccaacgtct aactaaacta gagatgaatc atcaaccaaa tgatgaatta atagaatata
      361 ttgatgaaat cctacaggag ttgattacag ggcatggaga ttatggttta gtaatcactg
      421 attgtaatga atacaatgag gacttacttt tattagaaaa aaagtgggaa gatttaaata
      481 ttgaaattaa aaaagtaaga atgaaagaac agaataacca gttattaagt ataagtgaag
      541 agtttttttc tcttgcaaat gatacagtgt ttaaaataga aaacttttct aaagaaaaaa
      601 gtaattactt aatgacttta ataataatta tatctattat aggtatatta gcatgtatca
      661 tattgatttt acaatatagt aaaaaaatgg ttagactgga aaaattaaat gtagatttaa
      721 aaaatattgc atataaagat gaactaacag gagtaaatac tatagaaaaa tttaaattgg
      781 atgcaaatca aaatatttgt atacataagg ataagaaatt tgctgtattc tatattgatt
      841 ttgaaaactt taaatacatt aatgatatat ttggatatga ttatggtgac atgatactga
      901 aaagatatgc taatcttatg atgaatgaca tagggaaata tgaaatattt gcaagagaaa
      961 ttgcagatag atttgttgcg ttaagatgct atgtagataa ggaagattta gtggtaagac
     1021 aacgagatgt agacaatgaa ctaattaata taacaaatga aattaaaaat aaacatagta
     1081 ttactgttgt tagtggaatt tgttgtattg aagatgtaaa tgaaaaatta agtatagatg
     1141 gtttaataaa tagagcaaat tttgcacaaa aaactgttaa gaataaacca ggtactaatt
     1201 atgcttttta taatgacagt attcgtaaaa aaatgattga agaaaatact atcaaaagta
     1261 gaatacatga agctattgaa aaaagagaat ttatagttta tttacagcct aaagttaatt
     1321 tacatagtca aaaaataaat tgtgcagaag ctttagttcg ttggcttact ccggataaag
     1381 gtattatatc tccagcaata tttattccag tacttgagaa gaattttttt attgccttag
     1441 tagataagta tgtttttgag gaggtttgta aatggattag aaagaggtta gatgaaaata
     1501 aaccttttgt tcagatatca gttaatgttt caagaataca attttataac actaaatttg
     1561 tagaaactta ttccaatata caaaataaat acaggatacc taaaaatact atagaaattg
     1621 aatttacaga gagtgttgct tttgagaatc aaaaacatct tttggaaatt atacatgatt
     1681 tgcatgaaaa tggatttact tgttctttag atgattttgg aaaaggttat tcgtctttaa
     1741 gtgttttaaa agatttacca tttgatgttt taaagcttga ttctatgttt ttcaaggcta
     1801 gtttagataa agataaagag aaaatagtaa taaaaaatat tgtacatatg ttaaaagaac
     1861 ttaatatcac aactgttgct gagggcattg aatgcaagga acaagttgaa tttcttaggg
     1921 atattgggtg tgatttagtt cagggatttg tattttataa accaatgcca atattagaat
     1981 ttgaaaaaat attggataaa gagtttgttt ataatagcta aaatgtactt tataaaatat
     2041 gaactaatta agtaaaatta ttttgtactg aataaatttt tactttattt acagaataat
     2101 gattatttac agaaaagcgc gaaaagtgta ttcctttaag tgtgggatgg atagcacaaa
     2161 tagaggagaa aaaatattgg ccatataaga atgcataata atataaatat ttaaaaggtc
     2221 agcattatat aaaaattaaa aagaaaataa ataatttttc tataaaaaag agactatgag
     2281 aaaatttact catagtctcc taaataatgt atttattttc ttcctgcaac ccattttaat
     2341 ccccaatgaa attcagtatc catttctcta tgacctttga agtatttaac tactttttca
     2401 acactgaatg tctcatcatt tacattttct atcaatgcta ttgaacacat attatagcca
     2461 tttttatgtt catcaagttt gacaactaag tctgaatctt gtttttgttt gattgttaca
     2521 attccatcag cttcagacca gttagctaca ccttcgtata taaaagcata aataagtatt
     2581 ctttttatat cttttatttt attaccattt attcttaaat tttctccttg tgtattactt
     2641 cctgttctgt catctccatc taattgagca aaaggtggat tgtctaaact accaaaggca
     2701 tttcctaatg cttgaacagc accttttaca ccatttttca tctcaaatag gcaacccaaa
     2761 tctaaatcta tattactgct tcttaaagag ccaaaaaaac cctttttttg tactttttga
     2821 ttccaattta aattaactaa aatttcacct aaatcgctat tttccttttt tgtaaggttt
     2881 attttttgtc ccttttttaa ttctatagcc atagtaatct tcctccacaa acatagattt
     2941 atttaaatgg tagcacttaa taagtcaata ttcaacaaga ttagaataat aatattaaaa
     3001 ttatactaaa tatactaaaa agtaggaatc acaatacctt tgtatttttc ttcaattaag
     3061 cttcttgctt tatctgatgt aagaacatct tgtagaactt taatcttttc actgtcttta
     3121 tcttcagttc taaatgctaa aagatttgca tactcgtcat ttataggagc tgtataaatt
     3181 ccgtttttct tggcatcaag accagcaggt acagcataag tagtatctat aaatgcagca
     3241 gtcacatctg gaagagaaga tggaatttga gcaatatcta actctttgaa ttgtattccc
     3301 tttggatttt ctattatgtc cttaggacta actaatccat cccctttagg taacgttaaa
     3361 agtccaatac tttcaaggta tctaaggttc ttagaacaag aagatgggtt attgcttatt
     3421 gcaattgtat ctcctttttt aaattcatca acagatttta tcttataaga gtaaagtatt
     3481 gctgggcata catatagttt tttaccagcc attattttgt aacctttggc cttaacagct
     3541 tcttttagat aaggttcatg ttgaaataga tttgcatcaa tttcaccatt atttaaagct
     3601 tcgtttggaa gtatgtaatc atcaaagttt ttaacttcta aagtatatcc tttttcttta
     3661 ataagtggtt ttaattcttc taataactct cctcctggaa ctaaagtagc accaacaaca
     3721 atttttttat catctttagc tttagaacat ccaaccacag ctaaagttag tactagacat
     3781 aataacaaac ataataattt tttgaatttc atataaattc cctcctgaat ataattgatt
     3841 agttaaaaat aaaataccat tctttatcac actgcaaaat aaaaataagc ctgagtttaa
     3901 actcaggctt taaaagctaa tttagactca tctttcagta taatactgca ggatttagca
     3961 ccaatgtata taaaaatata ctggttgctg ggtttcatag ggccagtccc ttcaccactc
     4021 tggataagaa gtatcaattt ttaattatgt tataaatatt atcatgattt tttacgtctg
     4081 taaacataat tttataaata ttttaaaatc tttaatgtat tttatttaac acctaaaatt
     4141 ccagaaacta gaatatcagt atcagcacct aagaaatagt catttatttc aatagaatcc
     4201 agagtatttc tcaaagcatt ttcttcatgc cttataccaa taagtttatt ttctacaaat
     4261 gcaacatcac attttccaaa aaaatctcca aaaaatttta tactagttat tatgccttta
     4321 tcaacattta aattaaactc aacatttcca ccagaatatt ttaattcatt tgaaagagta
     4381 tattttggtg aattaccaaa attccaatcc caagtttcgt attttgattt agtaagcttt
     4441 tctatttgtt ttatatcatg ctcactcaaa caataattag tattatcagt atttgtttta
     4501 gaaagataat ccattatagc atctttaaat tctaatatgt ccatttttga atgtaaatgt
     4561 tcacttatat tggttacacg agattttaca gattttattc cttttccttc aaattttata
     4621 ggtttaacct ttaatgcttt tgagaggtca tttatttcag atgaaaataa aagagtccca
     4681 tgatgcatta ctttgttttt gtagttgtat tgagcatttc cagaaaattt cttgccatct
     4741 ataagaaggt catttcttcc agaaaattcg gcattgacat ctagtgtttt aagtaaatct
     4801 attatgggct gagtgaatct tctgaaatca ctaaaattat tattgttaca agctataaat
     4861 gtaaaattta tatttcccaa atcatgaaat acagctccac caccagactg tctcctaacg
     4921 acaggtatag aatattcttt tatatattct aaatttattt cagaaagtgt gttttggttt
     4981 tttccaacaa ttatagaagg ttcattcctc cataaaataa ataagtcttc atcaaaattt
     5041 tttagaagat attcttccat tgctaaatta aaataagggt ttgttttttc attataaatt
     5101 aataacatta aagtagttcc tccatagaat tgataaaatt tttattatga atacaattaa
     5161 ttttataatt cgacaataat ataaaaactc ctttaaatta gaaattttaa aaactacctt
     5221 gcaaagttta aataaataat atatactcaa aatatgttta ataaaagtga atagaagggt
     5281 agaggcgcaa taatctatca gtactaataa ggagttaagc acagagagtt attaggaaag
     5341 gagatattgc cgaagtttta taatcgatgc tttaagatta tattgctggg ggtacataga
     5401 aaatatgtac aactgtcaca ggtttatttg tggtgagcta tcatttaagg aattatatta
     5461 tatttatttt tactattgtt gatatattgt aatgtttaag ccttgagtgt ttaccactca
     5521 aggctttttt agttttattg taacataata ccctgtacag ttcacaaaat taaaaggaga
     5581 gggagattta tatgaggaac aaaaaaatta atattatttt tctgacgaca atcatgttca
     5641 taatgtcaac agtaatggtt tttgcagaag aagatataga cactattgca ctagccaatg
     5701 cagaaaaatt tgggatttta actttaatac caccattagt tgctataata ctcgcattta
     5761 taactaaaaa cgtaataatt tctttactta taggtattct ttctggtagt tttataatta
     5821 aagctagtgg tattaatgtt tttgcgacat ttatacaggc atttttagat ttagtagata
     5881 gagctttagt atctttagca gacccttgga atgcggggat aatactacag gttttgtcaa
     5941 ttggaggagt aataaatctt gttgctaaaa tgggaggagc taaagctata gctgaagcac
     6001 ttgctaaaag agcaaagtca gcaaaaggga cacagcttat aacatggttt ttggggcttt
     6061 tagtattctt tgatgattat gccaattcac taatagtagg tcctatgatg agacctgttg
     6121 cagataaaat gaaaatatcg agagaaaagc tagcatttat aatagatgcc acagcagcac
     6181 cagttgctgg tttagctata atatctacat ggataggtct tgaggttggt ttaatacatg
     6241 atgcttttga aagtataagt atagatgtag atgcatttgg tatattttta aatacaatac
     6301 catttagatt ttacaatata ttaatattag ctttcattgt aatatcagca ttattattaa
     6361 aagaatttgg tccaatgaga aaagctgaga taaaatctag aagtagaaaa attagtatag
     6421 accttgatga aggcgtagaa gagttggatg atttagctcc aaaaaatgga gtaaaattga
     6481 gtgtatggaa tgctataatc ccaataggaa cattgataat agttgcatta gcatcatttt
     6541 attatagtgg atatacatct ataatgggag gagacgataa ggctctgata caactattta
     6601 caaattctcc atattctttt gaagcaataa aagaagcatt tagtgcatct gatgcatcta
     6661 gagcattatt ccagtctgca cttgttgcaa gtttagttgc tattataatg gctgtagtaa
     6721 aaaagatttt tacaatatca gaagcaattg atgtttggat agatggaatg aaaagtttag
     6781 taatcactgg tgtaatatta atacttgcat ggtcattaag ttcagtaata aaagagttag
     6841 gtacagctaa atttttaata cacctactat caggttcact accaccattc ttattaccaa
     6901 gtttaatatt tggtttagga gcaataatat cttttgctac tggaacagct tatggaacaa
     6961 tgggtatact tatgccactt gcaattccat tagcatactc attaaatcct gatatgtctt
     7021 atgtaatagt aagtactagt gcagttttaa caggtgcaat atttggagac cattgttctc
     7081 caatttcaga tacaacaata ctttcttcaa tgggagctgg ctgtaatcat atagaccatg
     7141 ttaacactca aatgccatat gctatattta cagcagtaat cactattgta tttgggtata
     7201 taccagctgg acttggattg ccaatatata tagtattacc agtggcaata gctgctatat
     7261 ttgtaggtat tcaaataatt ggtaagaaag ttgatgaagc ggaaatagaa cttgtagaat
     7321 aaagatataa ataagtttca ccttttatta tcactatttt gttagttttt agataatttt
     7381 aaaaagttat tgaaaataat ggcgaaatat agtaaaataa aagagtgata agcggatgtg
     7441 gcggaattgg cagacgcact agacttagga tctagcgcca ttggcgtggg ggttcgactc
     7501 ccttcatccg cactttacag tacttaattg tactgaaaaa aactggggtt gctggctttc
     7561 ggagtgggct atcccgtttt tatttttttg tatatataat agattggcac tttagtataa
     7621 ggtgcttttt ttataggcaa aaacagtaca tttatttctg ttttattatc attcatctat
     7681 tagaatatac tttagttaag aatgtgttaa taagcagtgt tcacattctt gataaaatgt
     7741 ggaataatat gtatttttac taagacggaa atgaaataat taaaccatta aataatatta
     7801 taagatgatg aataagtaga taaatcaaat tttgtaggag gatattatgg aaaaatatat
     7861 atggttgttt ccattacttt ttatttttca tgatatggaa gagataatag gttttggaat
     7921 atggcttaaa aaaaataaat caatgcttga taagaagtat ccatttatca gtaaagttta
     7981 tgaaaattat agtactgaag gcatggcatt tgctgtgttt gaagaattta ttttatgcat
     8041 tattttctgt atacttacag ttataacaga gaatcagtat gtatatctac tatggttagg
     8101 tagttttata gcatatacct tacatttggt aattcatata gggcagtcaa tcataataag
     8161 aaaatatata ccttcactta ttaccagtat catatgctta ccaataagca tttggtgtat
     8221 aagtaagagt atttatatag ttgattgtga aatgagtaca acaattctgt acagtataat
     8281 tggcattata attgtagcat tgaatttaaa gtttgcacag tcgctgattg gaaaatttac
     8341 aaagtggatg tcaaacatat aaattttagt ttattggaca gttcaaggaa tcagaaattg
     8401 gaattttata gtattaatat tttatggtat taatataatg tataaaattt tatagcaatg
     8461 tatttcataa gtataaagtg actagaattt taagaaatat acaaattcta taaaaactaa
     8521 ataatttcaa gtatatttta aaaatcttat ttttcacaag tttaaaattg taaacgattt
     8581 catcaagtct atgaatatac ttttaacatg ttatagtaaa tctcaatata aatttacatt
     8641 tggttctttg tgtgttattt taataataaa ttaagtgaaa atgttatttt acattaatat
     8701 ataatatctt tgcataagag tgaataatat aaaagagaaa taattgataa taaaaactta
     8761 acaaaaaaat taatacataa aaaaaaataa atataatgtg ttgacattta attgaaatat
     8821 tcatacaata aaattgaaaa taaatatttc caaatgagat tagcgagaga gtcctttata
     8881 cgaaaaggcg ccgaagaaga aatttaagaa atgccaattt ttagagaagc tttcaggcaa
     8941 aaatatcgct aattgatggg actctggaga gacttaagtt aagatactat aagtcaccga
     9001 agaagcaaaa taatattaaa ttattaaatc tttcaggtat acaaacaggg atatataggc
     9061 taggattatt ttccatgctg atatatcttt tttttatgaa aaagtacagg aaataatatc
     9121 aaagttactt aaatacagta ggtctaattt tctagggagg gttatatatg aatgaattga
     9181 aaagagtatc tttgtacaat atacacaaag agcttggagc aaaacttgtt gaatttgctg
     9241 gttgggaaat gcctttagaa tatgagggta taaataaaga gcatgagaag gtcagaaaaa
     9301 gtgcaggtat atttgatgta tctcatatgg gagaggtaca gataaaggga gcagagtcag
     9361 aaaaatttat acaaaaccta gttacaaatg atataagcgc attaaagata aatgatatta
     9421 tatatacacc tatgtgttat gaaaatggtg gagtagttga tgatttatta atatataaat
     9481 ttggtgaaga agattattta ttagtaataa atgcaggtaa tatagataaa gatgtagcat
     9541 ggataataaa acaaagtgaa ggatacaatg tagatataaa aaatatatct agtgaagtga
     9601 gtcaattagc aattcaagga cctaaagcag aagaaattct tcaaaaaatc acagatatag
     9661 acttaaactc aataaaattt tataagtcaa ttccatcaac aaaagtatgt ggttgtcctt
     9721 gtttagtttc aagaacagga tatactgggg aagatgggtt tgagatatac tgtaaaaata
     9781 aatatgtaga aataatttgg aatgaagttc taaaggttgg aggagaagat atatgtccag
     9841 ctggtctagg ttgtagggat actttgagat ttgaagcagc tctacctcta tatggacatg
     9901 aaataaatga acacatatct ccaattgaag gtggtttatc tatctttgtg aaaacaaata
     9961 aagaatcatt tattggcaaa tcaattttat caaaagaaaa agaaagtgga gctaaaagaa
    10021 agttagtagg atttgaaatg caaggtaaag gaatgcctag aaatggatat gatataagga
    10081 ttggtgataa aacagttgga tttgtaacaa caggatgtgc atctccaacg acaggaaaaa
    10141 ttttaggtat gggaattata gattctgaat atgcaaaagt aggaaatgaa ataggtatag
    10201 caatcagaaa aaaagttgtt cctgctgtga tagttaagaa acctttttat aaaaaacaat
    10261 ataaaaaaga taatattata ttaaacaaag aaaataaatt ttcatatata cctgctacaa
    10321 gtgaagataa atcaaaaatg ttaaaagtag ttggattaaa ttcagttgat gaattatttt
    10381 cagatatacc agaagaagta aaattaaaaa gagatttaaa tttggaaatt ggaaaatctg
    10441 aattagaggt aagcaaaata gtaaaaagat tgtcagaaga aaatttaagt ttagaagact
    10501 tgacttgttt tcttggtgct ggtgcatatg accattatat accatcaatt ataaaacata
    10561 taacttcaag atctgaattt tatacagcat ataccccata tcaagctgaa ataagtcagg
    10621 gaactttaca agtagttttt gaatttcaat ctatgattgc agaaattaca ggtatggaaa
    10681 ttgcaaatgc atcaatgtat gatggagcaa ctgctgcaat agaagcatgt ataatggcaa
    10741 tgaatcagac tagaaaaagt aaaatagtag tgtcaaaaac tattcatcct gaaactctgt
    10801 cagtattaag aacttactta caatataaag attgtgagat agtagaaatt gacttttgta
    10861 atgaatatgg gactacagat atagaaaaat taaaagcttc tgtagacaaa gatacagcat
    10921 gtgttcttat acaaactcct aatttctttg gaattattga agaaatggaa gaaatagaaa
    10981 agataactca tgaaaataag gctatgttaa ttatgagtgt agacccaata tcattaggag
    11041 ttttaaaaac accaggtgag ataggtgcag atattgttgt tggagaagct caatcacttg
    11101 gaaaccctct taattttgga ggtccttatg taggattttt agctagcaaa tctaaatata
    11161 ctagaaaaat gccaggaaga atagttgggc aaagtttaga tgtagaaggt aaaattgcat
    11221 atgtattaac tttacaaact agagaacagc atgtaagaag agaaaaagca acttctaata
    11281 tatgttcaaa tcaagcttta aatgctcttg tagcttcaat atatatggct actatgggta
    11341 aagaaggatt taaagaggtt ggtatgcagt ctatgaaaaa agcacattat acctacaata
    11401 aacttgtaca aactggaaaa tataagccaa tatttaaagg taagtttttc aaagaatttg
    11461 ctgtacaagg aaatcttaat atagaaacta taaatgataa actcttagaa gaaaatatat
    11521 taggaggtta taatttagaa tacaattatc cagaattaaa aaattcaact ttgctttgtg
    11581 tgactgagaa aagaagcaaa gaggaaatag ataagttagt tggaataatg gagggattat
    11641 aatgaaagaa tacaatagtt tgttgataga tatatctaaa aaaggtagga aagcatactc
    11701 acttccaaag ttagatatag atgatataaa aatagaaaat atgattgacc aagatatggc
    11761 aagacaaagt gagttgaatt tgccagaagt tggtgaatta gaattagtta gacattatac
    11821 attattatca aataaaaact ttggagttga tacaggtttt tatcctttgg gttcttgtac
    11881 tatgaaatat aatcctaaaa taaatgaaga tatggcagca cttccaaact ttactggaat
    11941 gcatccatac caatcttcgg atacagctca aggttctctt tctttgatgt atgatttatc
    12001 aagaagactt gcagaaatta caggtatgga tgaagttaca ctacaacctt cagcaggttc
    12061 tcatggtgag tttacagggc ttatgattat aaaagcatat catgaaaata gaggtgacca
    12121 taagagaacc aaagtgataa ttccagattc tgcacatgga acaaatccag caagtgcagc
    12181 tatggcaaac tttgatgtga ttcaaatagc atctgataaa aatggagctg tagacataaa
    12241 tgcattaaaa gaggttttga atgatgaagt agcagctctt atgcttacta atccaagtac
    12301 tcttggttta tttgaaaaaa atataaaaga aatagctact cttgtacatg aagcaggagg
    12361 acttttgtac tatgatggtg caaacatgaa cgctattatg gggataacaa gacctggaga
    12421 tatggggttt gatgtggttc atcttaatct tcataagact ttttcaactc ctcatggagg
    12481 tggaggtcca ggagctggtc ctgttggagt taaaaaagaa ttagtacctt ttttaccaat
    12541 accagtaatt gaaagaaaag aagataagta tgtactaaat tatgatagag aaaaatctat
    12601 tgggaaaatt aaaaatttct atggtaattt tggagttctt gtaagagctt acacatatat
    12661 actaactatg ggaagggatg gacttaaaga ggctagtgaa atggcagttt taaatgctaa
    12721 ttatataaag gaaagtataa aagatgatta tattttgcca atagatacct tgtgtaagca
    12781 tgagtttgta ttaggtggat tgccaagagg agaggccaat ataaaaacta tcgatattgc
    12841 aaaaagacta ctagattatg gataccatcc tccaacaatg tactttcctc ttattataaa
    12901 tgaggcactt atgatagaac ctacagaaag tgagagtata gagacattag atagttttat
    12961 agaagctatg aaaagtgttg ctaaagaggc aaaggaagaa ccagaactat tgaaaactgc
    13021 tccacataat actttggtca aaagagtaga tgatgcaagg gcagtaaaga aacctatact
    13081 aacatggtca caaagataaa tagttaaaaa gtagagattt aaagtataaa gagaattaaa
    13141 tttgtaagaa aaagctatct aacttaatct agtttagata gcttttttgt atattagtat
    13201 gtatggtatt ttctatataa agttttaaat tatattagtg cttatataga atattgatat
    13261 gatgattatg tatagatatt aataagaatt gttgaattta tcttgcctta actttattta
    13321 ctcttttata ttaagaaaaa cttaactaaa atatagcgaa attcataaaa atttaggaaa
    13381 tcttaagaat tataatattt aaatctttag atttatattt tattatataa actatagaat
    13441 aataaaataa aaaaattgag aattttataa tatatttgta gatttgagta taaaaaagtg
    13501 ctaagttgaa aggagtaaaa tatggataag gttaatttag aaaggtatag ttgtgatata
    13561 aatgttggtt taaataaaaa tcaacttcaa agtagaatag atgataattt aattaatgca
    13621 tttgataatg gaaaaaccaa aacatataaa caaattttta aagacaatat atttacactt
    13681 tttaatctca taaatattgt tttagtctgt ttattagttt cagttggttc ttttaaaaat
    13741 accttattta tatttattgc tgtaattaat actattatag gagtaataca agagataaga
    13801 gctaaaagat tattggacaa tctatctgtt atagtttcaa ataaggtatc tgtcataaga
    13861 gaaaaagaga aaaaagagat tgatgtacaa gaattagtat tagatgatat tatgatttta
    13921 aaaacaggta atcaaatttg tgcagattca aaagttttaa atggaaaatt agaggtaaat
    13981 gagtctttgg ttacaggtga atctgatatt attataaaaa ataaaggcga ttttttatat
    14041 tctggaagtt ttgtagtttc tggagaagct tttgtaagag ttgaaaatat aggaaaagat
    14101 aactatgcaa ataagatagc taatgatgca aagatttcta aaaaacataa ttctcaactt
    14161 agaaattcat taaatgcaat attaaaaatt gtaggtataa taataattcc tttaggaatt
    14221 atattgtttg caaaacaaca ttttggaaat ggagattcta tagctacatc tgtagtaggt
    14281 acagtggcag ctgtaaatgg tatgatacca gagggattaa cattacttac gagcattgct
    14341 ctagctgtag gaactataaa acttgctagt cataaaactt tagtgcaaga actgtactgt
    14401 gtagagactc ttgctagagt tgatacacta tgtcttgata agacaggtac aattacagaa
    14461 ggtaaaatgc aagttgatga tatagtaatg ttggaagaag tggatattaa tgaaataatg
    14521 gggaatatat gtaattctct caaagatgat aatgcaacac taaatgcaat aagagataag
    14581 tataatgttt tagatacgta taaagctaag aatttaatac cattttcttc agaaagaaaa
    14641 tacagtgggg ttacctttga aaataaaggt acatatttat taggtgcatt tgaatttata
    14701 tttaaggaaa aaaataaaaa acttaggata gaagttgaaa gatattctaa aaaaggaaac
    14761 agagtaatag ttttagctca tagtgattct tatatggaag ataataatat tcctgaagat
    14821 ataaagccac tagcttttat attgatattg gataaaatta gagatgaagc taaggaaact
    14881 ttagaatttt tttacaatga aggtgtagat ataaaaatta tatctggtga caatcctata
    14941 acagtaagtg aggtagctag aaaagcaggt cttaaaacag ctagtaattt tattgatgca
    15001 acaacactta aaaatgatag tgatatatac aaagcagttg ataaatacag tgtttttgga
    15061 agggtaagcc cacaacagaa aaaacaaatg attttagcat taaaaaaaca aaatcataca
    15121 gtagctatga ctggagatgg tgtaaatgat gtgctagcac ttaaagaagc ggattgtagc
    15181 attgccatgg catctggaag tgatgttact aagaatgtgt ctaatttagt cttattagat
    15241 tcaaactttg catcaatgcc aaaggttgtt atggagggaa gaaaagtaat taataacatt
    15301 caaagagcat cttctttgtt tttagtaaag acaatttttt cattcttctt gtcgttatta
    15361 acattattct tcttcagtag acagtatcca tttgttccta tacaattatc actcattggg
    15421 gctgtaacag tgggtatacc atcattcttc ttagctcttg aagcaaacaa aagtagaatt
    15481 tcaggaaatt ttttactcaa tatattaaaa aatgctttac caggagcact atgtgtgaca
    15541 gtaaatgtaa ttattgttta caatatagtt gaatatttag gttatagttc aagtgtattt
    15601 tccacaatgt gtgctatatt aactggtgta tgtggtcttt taatattaag acaacaatgt
    15661 ttgccattta ataaagaaag attattttta atagtaagta tgactattgc atttgttatt
    15721 gggattctat ttttaggtgg attattctca tttgtagcat tgagtagaga attaattctc
    15781 ataactatgg cattagcaat attaatgcca attatgataa aagttatgtt atttgtctta
    15841 gatgagatat tagagacaat tgttccagat ttaaattaat tttaaaatga tataaataag
    15901 agcagtttta gttgaactgc tctttttaga tgtgtttaat tttatgtaag aatatgtatt
    15961 tttattaatc agctatatat gagcaacagt agtcaacaac ttcattaacc tttaagtcaa
    16021 aacttgaatc actaggaaca ttaaattttt gcccttcttt ataagttaca aactcattgc
    16081 ttccaggcag ttttacatcc atacttccag caagcatctc cattatttct tcttctctag
    16141 tagaaaaagt gtattctcct ggcaacatta atcctaaagt ttttctttct ccattaggca
    16201 gtattattac tctgctactt accttaccat caaaataaac gtttgccttc tttactgcag
    16261 taacattctt aaattcactc attagataaa acctccaaaa ttatttgtac atatatatta
    16321 tataaaaata atgtaaaaaa tgctatatat ttacaaaaaa atttagtatt tattaaaaaa
    16381 attaataaga ataaaaaaga gatgaaattg cttaaattga taaaaaatat aaaataaatt
    16441 gcaaagtaag caagaaaaga ataagttgaa gagttatata ttataaatat taaaaatttt
    16501 agtattaata aaaaagatat tacattaata tgaaaatata ggagattaga agtgtttgat
    16561 aattacagta catgttagat atatcattgt aaattaacat agctttgaat ataataatgt
    16621 tcagaaggca atatttttga ggtctgaatc caataataat atagtcttat gattgtagat
    16681 atcaaaaaat gtaagttact aggaatacgt tttgtagatt tatatatgat taataactaa
    16741 agctataaat aaacaaaata aaaagagtat gtttcaacct atagctttta gatttaaaat
    16801 atactctttt attaattata gttgtataaa tta
//
LOCUS       DAYASV010000088        16260 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYASV010000088 DAYASV010000000
VERSION     DAYASV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.089"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(734..1372)
                     /locus_tag="ACGI68_002622"
     CDS             complement(734..1372)
                     /locus_tag="ACGI68_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0139050.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFTLWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVVIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(1375..2232)
                     /locus_tag="ACGI68_002623"
     CDS             complement(1375..2232)
                     /locus_tag="ACGI68_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139051.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(2234..2608)
                     /locus_tag="ACGI68_002624"
     CDS             complement(2234..2608)
                     /locus_tag="ACGI68_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0139052.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYQED"
     gene            complement(3770..4960)
                     /locus_tag="ACGI68_002625"
     CDS             complement(3770..4960)
                     /locus_tag="ACGI68_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFH0139053.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(4976..6931)
                     /locus_tag="ACGI68_002626"
     CDS             complement(4976..6931)
                     /locus_tag="ACGI68_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFH0139054.1"
                     /translation="MEKIKIESSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGIDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     SEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(6948..8993)
                     /locus_tag="ACGI68_002627"
     CDS             complement(6948..8993)
                     /locus_tag="ACGI68_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFH0139055.1"
                     /translation="MKKITKERIENTMKKLFTSETFLEYQFISDCQISPDGGYTAFIV
                     KKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRNEKVKEAV
                     KNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRPIVEGLPE
                     EEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAITAPMFNV
                     VGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGFELYQNQV
                     ILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGAGQTTKVD
                     GEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCLGNGLPEV
                     YTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYEVGKKYPA
                     VLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQFGEIDYI
                     DFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRSIANWSGM
                     EGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYVDKCVTPTLFLHGEKDYRCYMQEA
                     FQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIKKDNIGQ"
     gene            complement(9366..10016)
                     /locus_tag="ACGI68_002628"
     CDS             complement(9366..10016)
                     /locus_tag="ACGI68_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0139056.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(11000..11332)
                     /locus_tag="ACGI68_002629"
     CDS             complement(11000..11332)
                     /locus_tag="ACGI68_002629"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0139057.1"
                     /translation="MAQPFEVEPKLDELIEWYYSQSEVSIKVITEFHYRFELIHPFQD
                     GNGHIGRFVMLKQMLENNLPIKIVSWDSEELYRNSLNSCSLGNYVPLIEYLSSLEDFR
                     EVYKMLWK"
     gene            complement(11433..12113)
                     /locus_tag="ACGI68_002630"
     CDS             complement(11433..12113)
                     /locus_tag="ACGI68_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0139058.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(12110..13036)
                     /locus_tag="ACGI68_002631"
     CDS             complement(12110..13036)
                     /locus_tag="ACGI68_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139059.1"
                     /translation="MNYIIETNQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVKPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(13109..14026)
                     /locus_tag="ACGI68_002632"
     CDS             complement(13109..14026)
                     /locus_tag="ACGI68_002632"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0139060.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLLEKFYSRQINYNRSEKAMLQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRATVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLVNSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(14026..14715)
                     /locus_tag="ACGI68_002633"
     CDS             complement(14026..14715)
                     /locus_tag="ACGI68_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139061.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSEDMVIQYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(15123..15428)
                     /locus_tag="ACGI68_002634"
     CDS             complement(15123..15428)
                     /locus_tag="ACGI68_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139062.1"
                     /translation="MPSMIMEVVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     VGIIAAGIALIFFRNGIDSIDSIATYGVFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
BASE COUNT         5558 a         2741 c         1785 g         6176 t
ORIGIN      
        1 gaaaatatga atatagaaaa actttaataa gaaaataaat tatgaataaa taaaagaaac
       61 gcaatactgt atatagatga ttatttagaa aaataaaatc tcttttaata aaaataagct
      121 tcaaaataaa cttttagttc ataaagctta ggaggaacta aattgagtgc aagtgtatct
      181 actgttcaaa ataaatagtt cataaagcac tcttttttat attaaatcaa ttatattttc
      241 gtcatttcag caatgaaaaa gaaacttatc tctattttga gacagcttct ttttattaac
      301 ttcctacttt ttattagttt tctaaaattg aaaagcttta tttataggat tccttagatt
      361 tatattttga cgatataatt ttttaaaaac atcttaaaag atggtataca atacacacaa
      421 tcatactcat aattgaaaaa taatcaatgg aaaataaaat tactggatat tatctattaa
      481 aaaatacaaa ttgattcaca agaaacatat aatctataat attatgatgt tactctctcg
      541 caccaagtaa ctttattttc aagtaacctt ttaaattcat accatctaac ttcactcaat
      601 aaaaatggca atataaatat gtactctatt ttttactttt aaatatctga caagctttat
      661 atactattgg aagtaaaata caaagtaaaa cagcccaatc tggtaaatgt ggcatataaa
      721 agtccccctt accttataca agctccttat tactaaaaat tttaatagaa ataaacattg
      781 aagctaacag tataattaaa cttaaaagga acaatacaat attcactatc atggatggat
      841 actgatatat aaatggaatt tcaaaattgc tgtgagctat agttaatggt aatacaaatg
      901 atgaagccat gattatcacc acgaaaaaga attttgttgt ttcatatcct aatttatatt
      961 gaataggcat ataaactcca taaataatag aacttattaa taaaacaatc aaaatatatg
     1021 ttaagtcaaa agttcctatt tgtggaataa gtaaattttc aatccagtaa actaatgtac
     1081 aatagataaa taccattgca aataaaacat acttagattg aactaagcca cgtctaggat
     1141 aaggagttga acaaagtaat acagatgctt ttgaatattg actctccttt aataatagat
     1201 actggcacag cataaattct gaaaatataa tggatagtac aaatcccaat atttgtgcaa
     1261 atggaggtaa acgccacaat gtaaataatg ggattcctat tgcaataaac aatatcaaca
     1321 atacatattt ttttacaatc agaatatctt ttttaactaa atgaatcaac ataattactc
     1381 tctcctttca atatatgcaa gcataatatc ttcaattgta ggacgctcta ttaaaatatc
     1441 tggcatattt ttttgcactt cattaatttt tgaggtaatc cctataaaac cataacctgt
     1501 aatttctaaa tttaaaaata attttttgat ttcatcattt agaaattgaa tatccccttt
     1561 tacttttcta tgttcgtcca atagagtatc tttttcacgc tctaatataa tacgtccatt
     1621 gtcaatcata acaatcatat ctgctatttt gtctaaatca gatgttatat gagtggaaaa
     1681 gaaaacacca cgaccacatt ttttcatata tcctgttagt atgtcaagta tctgactacg
     1741 gattagtggg tctaaaccac ttgtaggttc atccataatt aaaaactcag ctttatgcga
     1801 taatgctaat gctaatgcaa atttcatttt cattcctttt gaaagtgtat caatcttttg
     1861 ttttgggtta agattaaaac gcttcatatt ttgttgataa tcttcttcac accaattatc
     1921 atatgaaggt gcaataatac ttttcatttc tgccattgac aactcctcat agaaatatga
     1981 atcatcaaat acaactccaa ttcgattttt taattccttc tcatttccat cgaaatcttt
     2041 accaaagatt tttatttttc cactatcacg ctttacgaca tccaatatcg aacggattgt
     2101 tgttgttttt cctgctccat taacacctat aaatccagtt atacaatctt ctggtaatgc
     2161 aaagcttata ttagataatg aaaaatcggt ataagtctta tttaagttat aaacttctaa
     2221 aatattttcc atattaatcc tcctgatata gaatatccat catttcttgt aactctatct
     2281 gtgtaattcc tagtgctttt gcatccttaa tcatatctgc cattttttgt tcaactatac
     2341 gtctctttga gtctcttagt atttctaaat ttccagttga aataaatgtt cctattccaa
     2401 cttggcgtgt aagaaagcct tcattttcaa gttcttcata cactcgtcta atagtcaaaa
     2461 tactaacttt taaatcatta gcaaaatttc ttatggatgg cattgcatcc ccttctttca
     2521 gttcttcagt aaatattgct tctttaattt gctctttaat ttgttggtat aatggcttat
     2581 ctgatgtgtt agaaatgagc actttcattt tttatcaccc cctttttatc attttcagtg
     2641 tgatgttgtt atatacacac tatacactta gagcactttt ttgtcaatat aaaagatgta
     2701 aaatattact atgacaaaat aaatatgaat gaaatattag agattgttga tagctataaa
     2761 cactttttaa aaccacataa gatagaatgt attaaatatt tattaaaagt aagaattgaa
     2821 tatctaaaaa aaagaggaat cctgtatgaa tgatttgttt gatgataaat taagttataa
     2881 actatttata aaaaacataa aggaataaat ccatttctaa ttacaaacat actaaaaaat
     2941 ttagatagta ataaatagat agtaataaat agatagtaat aaatgggatg aatttataag
     3001 aatttggata gaggaaagag ttattcaaga gtgtagacta gaggtaaact ctatattaaa
     3061 aaaatttaaa tttggatatt atgatgcatg gaatatttgc agaagtaacc ttgctataag
     3121 tatagaagat ttttttgatt acaaaagata atactctaaa atgtgaggat ttcaacattc
     3181 aatactgtac tttaaataat atcactgcaa aataccatcc accttttacc ataattgaat
     3241 accctaatta ttttaaaaaa gaaaacatta gaaacagaaa accttggata tataaaataa
     3301 aaaatactaa cttctaaaac acggatactc tttgataaat aaaacaaaaa ataattttaa
     3361 taattaaaat tggctatctc aaactagaga taagtctctt tctcactttt aaaacgttga
     3421 aaatatattc gatttcgtac aaaaaagagt gccttagaac taaaaagttc actttgtggc
     3481 actccctttt ttctcctctt tttaattttt tataaaaatt cttcaaaaat attattaaat
     3541 accctatatt atacataaat caaattaaag ttgcatttta gcaaataaaa acaaattata
     3601 actattagca tagcaaataa aaaatctgct ctcttataaa atcaaattat aattataaaa
     3661 agaaaatatt cttataacaa attagctaat ataaaattat tttataaatt aatactactt
     3721 tttagcttct cctataaatt aataccactt ttttaacttt gaaacctatc tacaacattg
     3781 gtaccataag accagctacc acaatagaaa tcatacaagc cataccacca actaccagct
     3841 taggcataat cttagacatc aaatatgctc tttcttcttc cgtttctcca acagcattac
     3901 acacttcatt tgaaattaaa tatgtagttg ggaatcccaa catctgacaa aaccctactc
     3961 caaaagcaag aggtttagaa ccgataattt tccaacctgg taacaccttc atcataagga
     4021 atataccaat gatagaagct gcaaacatac ccacaattgg tacaaaaaga ctaattaaat
     4081 ctgtcaaaga aacttttgct aaagaaggaa taacagctgc aaacacaacc atgttgataa
     4141 atcccttgat ttttcctttt tccaaaatgt cttttggcac aataccaata cgtgttacaa
     4201 taacaccaac aacaagacat acaatagaat aatttacatg aataatctct gaaagttcat
     4261 accctagata agagaataat gctataaaaa atatacaaac atttgtagaa taatatttat
     4321 cgtatttttc acagaaagct tgttttactt ttgtgcctgt attctctgct tctttcttat
     4381 cagtatcaac atttgctaaa tcaatgcctt ttgattttgc ttcatgatat tctactaaaa
     4441 gacggtttgc ctcttgtaac gctgctcttg aagctattgg tgtaccaata aacttctgaa
     4501 ttgcaaatac aactgttgct gtagctgctg caagtgtcag tcctttttct agtgctgctt
     4561 gtgtcataat agtagtagca ggtaaagctc catttataac tggtatgctt gtcaatactg
     4621 ttgattttcc aaacaaagga atacataatg agacacagac tataactgct acaattccaa
     4681 gccatgctgt caatactgtt ctccattcgt ctataaattg tttaacattc aacattgttc
     4741 ccatactaaa cataatcatt ggaacactcc aactagctgc tgcggtcata cctgcttttt
     4801 caataatatc agctggtatt gcttttgtga caaatagaat caaaaataaa agtagtgtaa
     4861 caaatacagc tgatactttt gcttttgttt tgcttgcaat taaatcccca atagcaaata
     4921 aaactaatac gacactaaac cataataaca tatcactcat agaataaccc ccttattata
     4981 aatatctttc aaaccaatct acaatagcat ttgctcttgc aattctatta gatggtcttc
     5041 catttctaga caatccatgt gtatctccgt ggaacaaata catttctgta tctacaccaa
     5101 gctgttttaa tgctgtaaac atttggaaag tatcccccat aaaacatcta taatccctat
     5161 cactatgtaa gaacaatgtt ggtgtcttaa catttttcac atatttaata ggtgaatgat
     5221 tccaaagaac ctcctcatga ttccaaggcg tcttatctcc acaagtctgc ttcataattc
     5281 tatccccaaa gtcaataatt aaagaagctg taatcaagtt actaatacct ctttgagaaa
     5341 ctgctgcttt gaaacggtct gtatgaccaa ttatccagtt tgtcatataa ccaccgtaac
     5401 ttccccctgc aacccctaag cggtctgcat caatcccagg atatttttca atacaagtat
     5461 ctacaaattc cataagatgc tcatagtcaa cataccccca tctttctacc atttctaaga
     5521 atttttcacc ataaccgtca ccaccatgag ggttacaata aatcacatac attccctttg
     5581 atgttacaca ctgcttcaag tgtgtaaaca tatagccata tgcccattta ggcccaccat
     5641 gaatgaacaa tacagcagga tatttttcgc cttctttagc atctataggt ggtataacaa
     5701 aaccatcaac ttcttcttta ttttttgcta caaacgtcaa tttttcaggt aatgaaacct
     5761 tatgagtatc taaatatggt ttagaaaaag cagtcatttt tttcattgta cctgtagtca
     5821 tatcaatgct atatatttct gctaactcat aatctttcaa cattactgtg tacatggtgt
     5881 tgtcgtaaat atcaaaagat gtaaaagcat ctacttttgt gatgatacgt atattacctg
     5941 tctttgtatc aaactcatta aattctgtca tagttctgca aattcttgca ccaataaatt
     6001 tatctcctat aactttatta ctacgtccac caccatatac tgcatctaca tccagtaatg
     6061 tagcatacat accatctgga ctagcaatca tatttatttc actagtactc aaatcaagta
     6121 taaaaatatc atgattctgt gtaatagaac ggtctaatgt tgaagcttgc aacattactt
     6181 tatctttgcc aatatatttt gccaaactta tttggtactt tccttgtggt acaagttctt
     6241 tggtttcacc actgtctata tgatactccc aaagtccaga aaaaggagtt gcacaatctt
     6301 cataaatctg accagtatat aaaattctgt ttttatccga agaaacatct atacctgcta
     6361 cttcacaaaa ttttggtgta atggcttttg ttatattttc accttcttga tataattcta
     6421 atgttacacg tcttctttga gaaaaatttt ctccatctct tataaaaggt ttatctgtaa
     6481 atgtccaata atctacaccc tctactgccc tatctggttc tgatttttta gaatccgttg
     6541 gattttttgt tgtcagtaac cacaatttat ttccaagtga tacaggtacg ctacaagctt
     6601 ttggaataga aaatgctttt tttgcttctc ctccttctat agaaatagtg taaaaatctg
     6661 ttgtacctgc ttttggctta tttcttttag atgtaaaaag tagttctgta ttagaaatcc
     6721 aactaaatga tgttacatct tttgtttgag tcatatgtat ttgtttttct gttttgatgt
     6781 ccatcacata taagtcatgt tcatagcagt ctttttcctc gttgatattt gatataatat
     6841 aagcaatatg ctttccatca ggtgaacaag aaatatcatg agggaatata tattcaaaaa
     6901 tatctgaact ttcaatcttt attttttcca tttttatatt ctcctttcta ctgtcctatg
     6961 ttatcttttt ttatatatac cgaaaaccaa tctagcattt caactaatct ttgtagcttt
     7021 tgttttggtc taccagaacg agacagttca tggttttctc cttcaaatag acaaagtttt
     7081 gtaggacacc catgaatttt caatgctgaa aacatctgga atgcttcttg catatagcat
     7141 ctatagtctt tttcaccatg taaaaataaa gtcggtgtaa cacatttatc aacataagca
     7201 agaggtgact gtttccattg aagctcgtgg ttttccatat gagaagcacc tgtttgtcct
     7261 ttgcagaaat aataaccaat gtctgttgta ccctccatgc cactccagtt tgcaatagaa
     7321 cgctgagcac aagcaacagc aaaacggttt gtatgaccaa ttatccaatt cgtcataaat
     7381 ccaccataac ttccaccttc aacagccaat tttgttttat caatgtctgt atatttttca
     7441 agtacagtat ctgtaaattc cataaaatct atataatcaa tttctccaaa ctgtttccta
     7501 atatctgcaa aagcattgcc acgaccttca gacccccttg ggttacagaa gaatacaaag
     7561 taacctgcac ttgtaaatac ctgtatctca tgcataaaat atggtccata tgtcaatctt
     7621 ggtcctccat gaatcgccaa tacagctgga tattttttcc ccacctcata atctactggt
     7681 ttatatacgt atccttgtat ttcccattta gaacttcctt ttgactctat gtattctggc
     7741 acagaaattt tatattcctc taaaatatgg tcatttaaat gtgttttctt acacaatgtt
     7801 ccattctcta ctgtataaac ttctggtaat ccatttccta aacaacccac acatacaata
     7861 ttgccatctt ttacatctat aaaatcaact gaacctgtct gtgttacttt ttcctgtttt
     7921 cctgttggaa tatgtattct ttcaataatg cagtccatat ctactgtaga agtgtaataa
     7981 atatattctc catctacctt tgttgtctga ccagcaccca ttttaacatc agatgttact
     8041 gtatttccaa tacaatgatg ttgatgccct gaaagaagtt tcatttcttt cgtatcaata
     8101 tcaatagtat aaaaatctcc attttctcca tttccataag ataaagcatc agttaggttt
     8161 agtatcactt gattttgata taattcaaaa ccttttataa tatacttatc tttttctaat
     8221 atacagacat ttgtgtttgt gtttgtatca aatacataga ccccattttt caaacctttt
     8281 acatctttaa attcttgccc tatatataag atttttccat cacttatttt catacctaca
     8341 acattaaaca taggtgctgt aattgccttc aactcattta tattactatc gtatacatat
     8401 aggcgttttc tttttctgct aatatatccc tccccattga cagcataagg aatttctttg
     8461 aaatgttcat aatgtctttt gttataagca tttatttttt tctgttgttc ttcttctggc
     8521 aatccctcta caataggtcg gtcattgtca taagaatcaa taactgcata caaacctttt
     8581 cctataggat tcaatttacc acctttgata gggagtctaa aaagttcttc tgcttctcca
     8641 ccatttaaag ataaagcata gtaactcatg cattgcttac cgtttttgac agcttctttt
     8701 actttttcat ttcgcaaagc agggaacaag atagtatttt catcttccca ggcatatgct
     8761 ccaacagagt ttatagacgt tatttgtttt aattcgccag ttttattgtt taatatataa
     8821 acttggcttg tgtaaccatt ctctttgata tcagcttttt ttacaataaa tgctgtatat
     8881 cctccatctg gtgatatttg gcaatcactt ataaattgat attccaaaaa tgtttcactc
     8941 gtaaatagct ttttcatagt attttctatc ctttctttag taattttttt caaactccct
     9001 accacctctt tcatttttta ttttaaattt aaaataaaaa attttaaatt taaaataaaa
     9061 aattttataa aggttatttt tttaggaagc cttctggctt ccttgtaata tttgtaattt
     9121 aaagtatatt taatccatgc ttaattcatg cttaattcat acttaatcta tacttaatct
     9181 atacttagtc catacttaat ctatatttaa ttcatactta gacttattta cactatattt
     9241 agattatatt tattagatta tatttattag attatattta gattatgttt aaactataat
     9301 tagattatac ttagactaca tttagcaact taatagataa gtatatatag taacttcaaa
     9361 acttattatt catcttcaca gaatctttta atagttcctt gaatatgatt tcttagttta
     9421 tctactgctt tttttgtatc tctgtcttca atgaatttta atatttcaaa atggtcttgt
     9481 actgttcctt cataattacg attttttaat ggtcgaatta aatagttcac tactataaat
     9541 cttccttgta taccaacata tacatcattt aaaatctcat tatttgcccc ttctacaatg
     9601 gctttatgta attccaaatc tgctagtgca agttctacag atttttcttg acgatagtac
     9661 tcctcgcatt tatcagagta tctttttatt acatctagct tttgttgaaa aacctcttcc
     9721 tcttcttgca tcattttttc taaagcatat gtttcaatca gtgttctcgt ttcacaaact
     9781 tgttttacca tatcatattc atactctggt acgtacactt taccatttgt cataatagta
     9841 agcactccat ctttatttag catttttact gcttgtacaa ctggtgttct gcttacttca
     9901 aatttttcac aaaggtcatt tagtaatatt ttttgaccta cagagtattc accacgaata
     9961 agactttcaa taatcttatg atataaaatt tcatctaaat ttgactttac tttcactact
    10021 ctcaccttat ttctattctt ttgccatatt atattgaatt actggacttt aattactttt
    10081 agtaatattt ttaaagtaga aattcttatt tgatagtact tttgtactac aatttaaatt
    10141 attgtactgt aaatcatgct cttatactat agattaattc attgtattgc aaatcaagtt
    10201 attgtactat agattaaatc gttatacttc aaattaaact attatactat agattaaacc
    10261 attgtacttc aaattaaact attatactat agattaaatc gttgtacttc aaattaaact
    10321 attatactat agattaattc attgtacttc aaattaaact attataccat agattaattc
    10381 attgtattgc aaatcaagtt attgtactat agattaaatc gttgtacttc aaatcaagtt
    10441 attgtactat agattaaatc gttgtacttt aaattaaact attatactat agattaattc
    10501 attgttctgc aaatcatgct agtgtactac aaattaaact ataaatgttt actatctctt
    10561 aataatttga ataatatgtt acaaaaagta tagcaccttt ttattttttt cgcaataaat
    10621 aaaaaattta aaatttttta ttttttattt tcttatatta tcaatttgaa atatgaaaga
    10681 ttaagactaa tagaattgat tcagttaatt gaaaaaaata ggtacctttt atgaaaattc
    10741 taatgataag catttttctg atgtaatggt aaattataaa tgataaaatt aaaaatcact
    10801 tttagtttta tgaactgtcc ccatattaag aaacaactaa gatacataaa aatagttata
    10861 aagagatagt taaactatat ccactatttt atatagttaa aatagtgggt atagtccaag
    10921 ctaagaataa ctaagaaatt agcgttaaat agcatagata aaaatacatt tatatataaa
    10981 ctttttttgc tgcaaacaat tatttccata acattttata tacttctcta aaatcttcta
    11041 aacttgataa atattcaatt aaagggacat aattacctaa actacaagaa tttaaggagt
    11101 ttctatacaa ttcctcacta tcccaagata caatttttat aggaagatta ttttctaaca
    11161 tctgttttag cataacaaat ctacctatgt gaccattacc atcttgaaat ggatgtatca
    11221 attcaaatct ataatgaaat tcagttataa ctttaataga tacttcactc tgactatagt
    11281 accattcaat taattcatca agctttggct caacttcaaa tggttgtgca atattattaa
    11341 aactctaata atattaataa tagctgatat aatagcaaat cattattatc ccgagaagat
    11401 acaattaata actaccttcc tatccacagt atctagtcaa tttcagtaac tttatgattt
    11461 agtatataat atataccaat cacagttata ccaactataa taaaaacaga aatcaaactg
    11521 tcttgagttg tatttgtacc agctaaaata aattgtctta aattgataaa aataattgaa
    11581 ctgacaaggg taacaggtat tgattttctt atcattccaa caacgaatgt ccatatacct
    11641 aatgagcaaa atacaataat acttttaatt gctcctggta tccaataaga taaaattgaa
    11701 atatgaaaat ctccaataac taaattaaaa tacttatcca acacaacaac gaatgtaaaa
    11761 caacagatat atccaactat cattgatgat atcataagag tcgtaatcaa aagtaacttt
    11821 gataaaataa gttttttacg gtcaagtgga taagagaata aaatcaagat tgttttattt
    11881 gtatattcat ttattatata tgccacattc aacacagcaa ataatataat atagtaaaaa
    11941 cagaaaaata aactgattgt cctaaatgta acatcataat catatattga agtagattta
    12001 tcattcaatc cagtaaagac aaaaaacata cataagaaaa tccccagcat tgaaaataaa
    12061 aaatatcttt ttagcccaac tttttttagc tctagtttca ttaaatgtat catagtgttt
    12121 ctttacctcc tgtacctgta gtttggaaga agtaatcttc taaagaactt tgtttacgtc
    12181 caatgctatt tataccaatt tgattttgaa taaatatttt ggatatttct tttacatctt
    12241 ttcgtaaatc atatatttca atacaacttt ccgaagtaat tttaaagtct tttattccaa
    12301 attctttttc taacaagtat ccagcaagct ccacattatc tacatctact tcaacataat
    12361 ctgtttgata tttgtgtata tctgacatag aaatttcttt aataagttta ccatcttgaa
    12421 taattccaat agtgtccgca atttgttcta cctctgaaag aatatgacta gaaataaata
    12481 tagttattcc ataatctgta tttaacttac ggaataattc tcgcatttct cttatcccct
    12541 ctgggtcgag tgcatttata ggttcatcaa gaatcaataa ttctggttta gtaagaattg
    12601 ctctagctat tgcaagacgt tgtttcatcc ctaaagaata ttttgaaact ttcttgtttg
    12661 attgctttga caatccgaca cattccaata cctctgatat atgttctttg ttataataac
    12721 ccatatattc acagtgtaaa tcaagattct gtttagcagt caaattctca tataaatacg
    12781 gattttcaat aatagtacca atctttttta atatttcaaa atcagtttct gccactttct
    12841 taccgaacat aactatttct ccagaatttg gcttaacaag atttagcaac attttcatta
    12901 ctgtactttt acctgccccg tttggtccaa caaatccata aatttctccc tttttcacat
    12961 gaatattaac agcattaaca acagctttat ctttatatat tttctttaat tgattggtct
    13021 ctatgatata gttcatcata ctccctcctt tctaaaaaca gtatacaaaa taaaattatc
    13081 ttttctctct tttaaatcta acttttttct aacttttcaa ttttactgta aaaactgttt
    13141 ttacattagg tctactattt acataaattg aacctcccat agagtttaca agcccttgag
    13201 aaatagccaa acctaaacca ttgccttttt ttctgtcagc agtataagct cttgtgaaaa
    13261 tatgctcttt ttcagtttca gaaatacctg aacctttatc ctcaatatct ataaaaacat
    13321 gtttttcctt ttcatatagt gaaatcccta aaaatctacc atctgaacca tattttatag
    13381 cattatcaat taaatttttg agtatacgtt gtaatgcttc tgtgtcaaca tttgcatata
    13441 taggtctatc ttcaatatta acttcaacat taaagttttc tttatctagc aaatcataaa
    13501 actctaaaat aatctcatga cataattgtg ttagattgga tttttgaaga tttaatatca
    13561 tatctccaga ttggattttt gccatattaa acaggttatt tattgaatta acaagctcat
    13621 ttgaatttgt ttgcattttt tccacagttg ctctcattga agaagataat tcttcttttt
    13681 gactttgaag atataacatt tcaatataac cctttaatac tgtgatggga gttcgtaaat
    13741 cgtgtgatat gtttgtaaat atttgcaaca tcgctttttc acttctatta taattaattt
    13801 gcctgctgta aaacttttca agaagtttat ttatatctac tgctgtttct tttacaatcg
    13861 aaatatcaga tggtactaaa atataattaa tttcatcatc atttgttatg tcacgaagtc
    13921 ttgaattgat atacttataa tcactttcca ttttctttga tttatttcgt tcttttaagt
    13981 agagagcaaa tagtacaatc gaaattacaa ctaaaaataa ctccattatt cttcctccat
    14041 tttgtagcca attccccaaa gagttttaat ataattattt ttttctatac catcagacaa
    14101 tttatttctt aacctgttaa tatgagtatt tagcacattt tcatttccat aataaggttc
    14161 tttccaaata gcgttgtata attgttcctt tgaaaaagca cgtccttgat tagtcgctag
    14221 taatttaaca atttcaaatt ctgtatgtgt taagtttaag tcaattccat tctttgaaac
    14281 tacatatttt gatatattta taatcaaatc tttatactga ataaccatat cttcagagtt
    14341 tcttgaactg ctgtacactg ttgcacgtct aatattagct ttaattcttg caagcaattc
    14401 tacgattgaa aaaggctttg taatataatc atctgcacca agattaagcc ctagcatttt
    14461 atctgtgtca ctatctttcg cagtagtaat aataacagga acaacactca cacttcgtat
    14521 atattttaat acttcaaaac cactagactt tggtaacatc aagtccagta aaataatatc
    14581 aaatgaataa tttttaaata tttcaatagc tgtatttcca tcaatggcag ttttaacaga
    14641 gtaattctca ctaatcagaa aatcgtatac catttctcga atattcttat catcttcaac
    14701 caataagatg tccattatta ttcaactcct tttcgtcagt atacttcaaa tgatatattt
    14761 attaaaaagg gttgtcaata aataactgct tatgatattg tatctgcaat ttggtgatac
    14821 tcttctaagt taaagttctt ttcaataagc tttattgaaa aaataattaa aatatagtat
    14881 actctcttat ctttatctga aatatacata attttctttt attaacatct acaattaact
    14941 attttatatt ataaaacacc ttttaactct tgctaaaaaa tattttatac ataaaaaaga
    15001 aaccacattc tagttttata cagaacatag tttctttaaa aataatcaaa tttggatttt
    15061 aaataattaa aaacaaactt ttatttgttt ttaattttcc aattttatag tagataattt
    15121 agttaattag ttcttaaatg tttatcttct ttagtaaatt tttttcttat taagaaatat
    15181 gcaactatta caacaagttc tattgctaaa aatacaccat aagtagctat agaatctatg
    15241 ctatctatgc catttctaaa gaaaattaaa gcaattcctg ctgctataat acctacagca
    15301 tttttcccta tagaattttg accaaaacca taaaaagaag ggaaatttcc ttttatttca
    15361 atgaatgtca agattaatcc aatagctata aatagtactc ctacaacttc cataatcatg
    15421 ctaggcattt tgacccctcc ttattaataa ttaactacta ctataaaaat atttattatt
    15481 tttataatta tattatatat gcgattacat gtttttggaa gtatatttgg aagtaatttc
    15541 atatttattt aacttaattt ttttgtgata aaatacttct aatttttata tagatattat
    15601 tataaatttt ctagtttatg gagtcattca ttctataatt cacaaatatg tattttttaa
    15661 tctattaaag tattcatttt ctggcttata agcatggact tcttatctat aagcttggtt
    15721 tttcatctaa taagttattt attctttaat ctatgagctt gatttttcat ctataagtca
    15781 tttatttttt aatctatgag tttggttttt catctataaa taagtcattt tttaatctat
    15841 aagtttagtt cttcatctat aaatcattta ttttttaatc tataagtttg gtttttcatc
    15901 tataaataag tcatttattt tttaatctat aagtttagtt cttcatctat aaatcattta
    15961 ttttttaatc tatgagcttg atttttatct ataattaatt cattctctaa tctattagct
    16021 tggtttttat ttataagtca tttattctct aatctattag cttggttttt acctataatt
    16081 aattcattct ctaatctatt agcttggttt ttatttataa gtcatttatt ctctaatcta
    16141 taagcttact tcttaatcta taaatcattc attttttaat ctattagctt ggtttttacc
    16201 tataaatcat ttattctcta atctataagc tttctttttc atctataaat cattcattct
//
LOCUS       DAYASV010000089        15458 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYASV010000089 DAYASV010000000
VERSION     DAYASV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.090"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            196..2094
                     /locus_tag="ACGI68_002635"
     CDS             196..2094
                     /locus_tag="ACGI68_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HFH0139063.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            complement(2222..3343)
                     /locus_tag="ACGI68_002636"
     CDS             complement(2222..3343)
                     /locus_tag="ACGI68_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0139064.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            3542..3874
                     /locus_tag="ACGI68_002637"
     CDS             3542..3874
                     /locus_tag="ACGI68_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0139065.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            3861..4919
                     /locus_tag="ACGI68_002638"
     CDS             3861..4919
                     /locus_tag="ACGI68_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFH0139066.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINENSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            4912..5982
                     /locus_tag="ACGI68_002639"
     CDS             4912..5982
                     /locus_tag="ACGI68_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFH0139067.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDIAVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSENGDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHNEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            6084..6620
                     /locus_tag="ACGI68_002640"
     CDS             6084..6620
                     /locus_tag="ACGI68_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0139068.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            6736..7443
                     /locus_tag="ACGI68_002641"
     CDS             6736..7443
                     /locus_tag="ACGI68_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0139069.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEELFMKVVAENRKRGQ"
     gene            7445..8161
                     /locus_tag="ACGI68_002642"
     CDS             7445..8161
                     /locus_tag="ACGI68_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HFH0139070.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIASILIILLFMWQIPLCMFLASKFGMFVTVLTNMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            8163..8921
                     /locus_tag="ACGI68_002643"
     CDS             8163..8921
                     /locus_tag="ACGI68_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HFH0139071.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSSVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFTSSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLICGFVTILAFVILCIWFSKWEGKKSEE"
     gene            9056..10444
                     /locus_tag="ACGI68_002644"
     CDS             9056..10444
                     /locus_tag="ACGI68_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HFH0139072.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGNLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVHNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGTLCIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(10520..11341)
                     /locus_tag="ACGI68_002645"
     CDS             complement(10520..11341)
                     /locus_tag="ACGI68_002645"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HFH0139073.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYNRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            11592..12746
                     /locus_tag="ACGI68_002646"
     CDS             11592..12746
                     /locus_tag="ACGI68_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HFH0139074.1"
                     /translation="MKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNMEEKVM
                     ESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYENEVKK
                     MKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQKDKENA
                     FRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGVELQLT
                     DEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGRFKKNS
                     IWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFVGYEKK
                     GRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            12929..13129
                     /locus_tag="ACGI68_002647"
     CDS             12929..13129
                     /locus_tag="ACGI68_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0139075.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            13550..13978
                     /locus_tag="ACGI68_002648"
     CDS             13550..13978
                     /locus_tag="ACGI68_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFH0139076.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(14121..14876)
                     /locus_tag="ACGI68_002649"
     CDS             complement(14121..14876)
                     /locus_tag="ACGI68_002649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0139077.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
BASE COUNT         6065 a         1708 c         2510 g         5175 t
ORIGIN      
        1 aaactattgt atgtataata ttttttccaa taaaaaatat tgcgaacttt ttatacttga
       61 tatataataa tataaataaa aacacgaata aaacacgaat aatttgcaag aaaaattcat
      121 aaatgtaagg aaaatgaaat ttatcttgta tttaacaaaa taaaatatac ataaaaatta
      181 tgggagagtg gaaaaatgga tagtctatta tacgtaatag agaaagataa acacactaat
      241 gaagaattaa gagaaatcct aaaaagcaat aaaaatataa gatttgtttc cttaatgggg
      301 gttgatttag gaggaaatgc aacagatgaa aagattcctg tagaattatt tttggaggat
      361 atagataaat ttttggaatc agctatacag actgatggtt caagtgttga gctttataat
      421 atagctacat taaataatgc gaaagtagat ttaatgccag ataaaagttg tcattggtat
      481 gtagattaca atatggaata tatagacgaa gaagtaggtc tacctgttgg aacattaaag
      541 atacctgcat ttttaataca tgataataaa aaagtatgtt ctagaggagt acttcaaaaa
      601 gcagataagt actttaaaaa gtcaatgtat gaaatattta gagaatatcc acatgtaata
      661 aataacatag gaatagattc agtagatgat atagaggaaa taatgttaac agcagctaca
      721 gaacttgagt tctgggttaa tactcctgag gataaagctg acttagagaa attatatgta
      781 tcacaaagcc taaaagagca atactggaaa agaactcatg gtattataag aacttgttta
      841 gagaaaagtt taattatact tcaaaagcta ggtgtaaacc ctgaaatggc tcacaaagag
      901 gtcggaggaa tacaaagttc tattagtata gatggaagaa caaaccatgc tatggagcaa
      961 ttagaggttt cttggaagtt ttctacaccg ttacaagcag cagacaatga attattagta
     1021 agagatgtta tagaggatgt atttacatca catggactag aagttacatt taaagcaaaa
     1081 ccaattcatg gagtagcagg aagtggtgga catacacatg taggagtaag tgcgaagtta
     1141 aaggatggaa gtataaaaaa tctatttgcg ccaaaagatt taaaagaaga ttatctaagt
     1201 gaactaggat atggggctct tatgggactt ctttacaatt atgaagtatt aaacccaatt
     1261 gtaacagctt ctaatgatgg atttaataga ttagttcctg gatttgaagc tcctgtatgc
     1321 atagttacat ctttaggtca tagttatgag attccatcaa gaaataggtc agtgcttgtt
     1381 ggattaataa gagatatgaa aaatccaaag actgtaagat ttgagttaag gtcaccaagc
     1441 cctctatcta acacgtattt agtaatagca ggatgttatc aaacaatgtt ggatggtata
     1501 aaagcagcag ctaagagtgg tcttagtaca aaagaacttg aaaaagagtt atctaaaaat
     1561 gtaggtgaag aaagtttcta tttagaaaaa gatagagcat atagagatga aaatgatgta
     1621 tttgaacatt atagtttgga agaaagaaat gcaagatttg gaataccacc ggctacagtt
     1681 tatgaaaata tgaaaaactt agaaatatat gcttcaaaat taaaatcttt aaaacaaggt
     1741 gatgtattta ctgatagtat aatagaatca tttaagattg gagctataga taagtggcaa
     1801 aagaaattaa aaactagaat aatagaggct gggattcaaa aaataagaag tattgtcaag
     1861 atacacacaa aagaaaatat ggatgcgctt gatgaggtag tttggaactc aatatcggat
     1921 ttaaagttca atattatgaa ggatacttta actagagaat cactattcac tagagtgaga
     1981 gaagctatag aaaataagga ttaccagact gcttcagatt tacaaataga attaaagaga
     2041 agtatggaag aaatacaaca actgtatatg cagtacaaga aaaatatata ctaatataac
     2101 aatttgattt tgatatgata aaggcaatca agtattatta aacactatga gtaaattctc
     2161 aagtgtaaaa gcatacttga ttgcctttaa ttttatgttt gaaatttttt aatagtacaa
     2221 tttaactgta atgattatct attaattctg atatataatc agcaattttc tctggatgaa
     2281 tgactgtttt aaattctttt aaattttttt tcataaagtt tattatagaa tcaaattcat
     2341 ttgatactaa tttataaaat atgtttttaa ggtctaggtc atcactataa atcatgccaa
     2401 gacccttatc tattataaat ttagcgtttt caatttcttg tccaactttt ggcattttta
     2461 cgagtactgg agtttgtgat tgaatagctt caaatatagt tgctcctcca ggcttagtta
     2521 gcattaggtc acattctctt attagattat acatgtcgtt tacaaatcca agtactttaa
     2581 tattttttag aggtttttta tgagttagat tgtcatataa ttttttgttt ttaccagtaa
     2641 ctattgtaat ttctatagaa ttactatgtt cttcaataaa ttcatctatc caatgcataa
     2701 aatcctcatc tatatcaaat agacctcttc ctccacctag aagtaatagt ctatatttat
     2761 cacaacatag ttctttactt tcaatttgaa atctcttatc aacaggaact ccagtaacct
     2821 ttatggagtc tggatttatt cctttctgaa aaaatctatt ttttatttca ggagaaggaa
     2881 caaaatacaa atctgtattt tcaaaaaccc attccatact atctacaaca tctgttataa
     2941 ctgtaagtgt aggtatattt atatcaggat tttttatctt aaagttattt acacaagcag
     3001 cagctagtgg aaatgtagag ataatcaaat ctggatttat atcagcaata tactcagaaa
     3061 gtttaggtgt atataattta tgagataaat catgtcttga atcaaacgat ttcttaaagt
     3121 aataatagta attatataat ccaggagtat attttgtatt gttttcataa agttttacca
     3181 taggcttatt catcatggga atacttgcat ttataaagtt ttgtataaca acattatagt
     3241 tactatattt ttcaataata cattctttga ctgcttttgc agcacttata tgtccagcac
     3301 caaattgagc agttaggatt aatattgttt tatcatcttt cattttagcc aatttatcac
     3361 cactttcata aaatatatct gattagagta ttatacattt atagaaggat ttaatcaaca
     3421 agaatattta aaaataacta ttgtgcagaa catagtactg tgttattatg aggatataag
     3481 aagttttaac atagtacctt gtaaaacaca atactgtgta tttaaagaaa ggatggagta
     3541 tatgaacaca caatttagaa agggtgtttt agaaatttgt gtacttgctc tcatatctaa
     3601 gaaagatatg tatggatatg agatagtaca taacatctct aaagtaatag atgtaaatga
     3661 aggaacaata tatcctattc taagaagact tacaaaagat ttgtatttcg aaacatatat
     3721 actagaatct aatgaaggtc ctgctagaaa atactataga ataacttcac taggaaaaga
     3781 gaatttatcc aatctgatgg aagagtggag agaatttgta aaggctgttg agattttaat
     3841 aagtgaagat gagggaggta atgaacttga ataaaactga gtttcttgaa attttaaagg
     3901 attatctaaa aaaagatttt tctgagagcg aagttaatga tatattgaga gattatgaag
     3961 agtatttcgt agatggtctt atagagggaa agagtgatat ggagataata gcatcacttg
     4021 gctcaccaaa atctatagca aaagaactta tcttacaaac taaagaaaaa aatattgaag
     4081 aggaaaaaat aataaataca gatgttttga atgataagtt tagtaaattt aaatcagata
     4141 tgaaaaaagg ttactctatc ttaaaagatg aagcaaaaga aaagtatagt aaatcaaaag
     4201 aaaatataga tggaaaaata atgaatacag acatattaaa tggtaaattt agtagattta
     4261 aatcaaatgt aaaaaaggat tattctatct tgaaagatga agctaaggaa aagtacaata
     4321 aatcaaaaga aaatataaat gataaactta caccaagtat agaagaaggt gtaggaggat
     4381 tatccagaca agttataaag gggttattag tagtgttatc tttatttcta attgttccag
     4441 catttgcgtt tgtatgtgct attgtatctg ctactattgc attagtatgc tcattaattg
     4501 cttttgttat attaattcca tttgtgatta aatttatagc aatggttcca gatgttatga
     4561 tgttttgtat tttcgcaacc atagcatttg tagggcttga gatacttgca tggcaaattt
     4621 tattatttgc cataaaacta ggtaaacaat tactaaataa ttatatacat tggataaaga
     4681 gaaaaaatat ttatataaag gcaaatgaaa agatggaaaa aagaaaaaaa gctactttaa
     4741 atgatgaaga tttgaataaa gacattaatg aaaattcata taataatgat tactacaata
     4801 gagaagaaga aagcataaaa gaaactgatg aatataatga tgatagtata aattttgagg
     4861 cgatatataa ggaccttgaa gatatagaaa ataagaaagg agaagataaa catgaataaa
     4921 aaacttgcta tatttgctgc agttttattg gtaataggta taatagggac aacatggtca
     4981 gggattttag ttatgccaag cctaataaac tttggtttag aaaaggaagc tgaattaaac
     5041 aaagaaaaca aactttatca agaaaaagta aatattgaca agttagatat agctgttgac
     5101 aatataaatg ttatgataag aaaaagttca aatgaagatg tgcgtgtgac aacaagaggg
     5161 aataatgaat tttacaaata cagtgtaacc ctacaagata agactttagt tgtaaaaggc
     5221 gaaagaaaat ctgaaaataa aattaaaaag attagaagtt tcgaccactt attaaataca
     5281 tctataaata gtatgttttc acgtgattat agagaaatta gaatatatgt accaaataat
     5341 gtagatataa atgcatcaag tataagttcg tacttattta tatctgatga tgtagattca
     5401 aataatataa catataaaac ttcttctgga ggttttagca gagctataac agaaaataaa
     5461 gtaaatagat tagaaaactt aaacttaata tctaatagca atcttcatct atcaacaaag
     5521 tcaattttag gcgttaaaaa tgtaaacata gaatcagagt cattggatat atcttcagag
     5581 aatggagatg tatttatcaa taatatagaa gagtatatac ctgaaaatgt aaatataaaa
     5641 gagaaaatag gtagaaattc aaattatgaa agtgaatttt acttgtactc tgatatgcca
     5701 attgctaagt tcctggacat agaagttcca aattctaagg taagacttga tataccagta
     5761 aataagtata aatttaattg tgatataaag tcaaaagaag caattgaaga atttaataat
     5821 gatgaagaat atgacaatga ttcatatgat tatgaacata atgaagaaca tagcaacaag
     5881 tatagaaata cgagggaaat aaaagggctt ttgaataaaa atctgtcgaa tttagagaaa
     5941 gaatatacta taaaaataaa ttcaaatagc ttagaattgt aaataattta ctataataat
     6001 ttataaattg atttaaaagt ataaaaatat aatctatact atggtataat aagtatcgaa
     6061 agagaattag gagagggata cttatgaata aaatagcagt aagtttttta ataattgcta
     6121 ctactttatt gagtacggca tgtatggatt atagcatcag tgcagttgag ctagttgatt
     6181 ctaaagaatc agcagttgta aagaaggatg aagatgcaaa agaagaaaca actagtaaaa
     6241 tgattaattc taaaaaaact actaaaatac ctatagagat aatttcaaag gatgagaaaa
     6301 tagtaaagta cttacagatt gatgaagaaa gttcacttaa ggataaactg cgattaatct
     6361 tagatacatt atcaaatgag tattttaatg gtttagacat ggaagtagaa gttaaggaaa
     6421 aagataatct tgttaaaata aacttgatag aaccagataa aaaaagtaga gtttcttgga
     6481 aagatgatta tttgaatgaa caaaatataa tatatactat aaataatatt attaaaaatg
     6541 tcatccaaga agaagataat agcatttgga ttgaagaagt agagatatac tacaatggaa
     6601 aattaataga attaagataa aaaaagatgt gtttttaaca catctttttt tgttttttaa
     6661 ttcttataaa atccttatat ttatgattta gaatgattct tgtaaataag tttgacaaaa
     6721 gaaaggatga aaattatgag taattatatt ttgcaggtaa aaggagtttc taaaaaattt
     6781 aaggagcaga tggcagttga taacatttca ctagcaatta aatctaattc tatatatgga
     6841 ttattaggac caaatggagc tgggaaatct actttattaa aaatgataac tgggatttta
     6901 aatccaactt caggaaaaat aatgtttgag ggacatagat ggactagaaa ggatttatca
     6961 aatattggtt ctcttattga gtctcctgct atatatgaga atttaacagc acgagaaaat
     7021 ttaaaagtcc atactacttt acttggtctt cctgactctc gcattgaaga agttttagag
     7081 actgtagatt taagagatac ctttaaaaag cgttcaggtc aattctctct aggcatgaag
     7141 caacgccttg gtattgctat tgcattatta aatcacccaa aacttttaat cttagatgag
     7201 cctacaaatg gtcttgaccc tattggtatt gaagaactta gagaattgat tcgttctttt
     7261 ccaaaacaag gaattacagt tattttatca agccatatct tgtctgaagt tgaacaagtt
     7321 gtagatgaaa taggaataat ttctaatgga gtaatgggtt atcaaggaga agtatcaaaa
     7381 gaacaaaact tagaagaact ttttatgaaa gttgtagctg aaaatagaaa gagagggcag
     7441 taatatggaa agatttatta gagtggaatt tcaaaagatg aaaggaactt ttggaatgaa
     7501 gttgatatgg atagttccag tgttttgtat acttttgtgt ggactaggtg gagggcaaaa
     7561 tgctgctttc aactggtggt atacgacatt tcttccagga gcattaacat taatatcttc
     7621 tttaatagta ttaaaagatg aaaaaagtaa atatcgtggt attttaccat tgccaattga
     7681 aatgaaagac gtttggactg gaaaaatgat ttactgtatt atttgttgtt ttatagcatc
     7741 aattatttct gcaataggtg tagtttttgt tgggataatc tataaagaaa ctatttcaat
     7801 aggtcaaaat atcattgcaa gtatattaat tattttacta tttatgtggc agattcctct
     7861 ttgtatgttt ttagcttcaa aatttggtat gtttgttaca gttctaacaa atatgttctt
     7921 tactattgtt ggagttgctg ttttctcaac tacagaaatg tggtatatgt ttccatattc
     7981 gattccttca agattaatgg ctgttgtatt gcatattctg ccaaatggtc ttcctgtacc
     8041 agagggaagt gaatttttaa ataatgctgt tattttgcca ggagtattaa tatctattgt
     8101 tttatttgta ttatttgctt tagctacaag ggtttggttc aataatagag aggtgaagta
     8161 atatgagaca gttaataaga atattttgtt ctgatttaat aaaattaaag agaaatttta
     8221 taatactaat gcacatttgc attgctttaa tggggatggg attgttttta tggtattata
     8281 aggtttcagg agctgataat atactaaaga tagtagcata tttacaagtt atagcaatag
     8341 cttttccttt attgtcaagt gttgtgtgtt caatgtttgt tgaacaagaa tattatgcag
     8401 ggaattataa acatatgtta acaacatcta atccaaaata tcttactctt ataagtaaat
     8461 atataatctt agtatgtctt ggttttgtag caacattagt gtctgtatta ggttttaaat
     8521 ttggtatatc taaggcttac tttacatcaa gcttttatat gatttcaatt ctaattttaa
     8581 ttgggtgtaa tttatttgaa tacatattac atttattctt aagtttaagg ttcggaaaag
     8641 gaacatcaat tggagtaggt atagttgaaa tgttactttc agcattatta ttaactggat
     8701 tgggttctgg tatttggcag tattttcctt gtgcatgggg tgttagattt ataactattt
     8761 ggtcgtcttt ttcaagttct aaaacggttg aatatataaa ggttgaatca ataaaagcat
     8821 atcaatcaac aggtttaatt tgtggttttg taacaatttt agcatttgta attttatgta
     8881 tttggttttc taaatgggaa ggtaaaaaat cagaagaata gagtaaaaat tgactgttta
     8941 tttatattat tatgttaaaa tataacttag caataaaaag atataaaata atcaaataaa
     9001 aaactggttg ggtattaatt atgaattaat taaactattg gaggacaggg tacaaatgct
     9061 tggaggtaaa aaatctaaca acaaaaaagt tgaaaagtta agaactattt ttataaaata
     9121 tttatcttta ttctttatta tgacaatttc aattgtactc cttttaatgt tatcattttc
     9181 tgtattatta tcatcaggtg taattttacc agctaattat gcagaaaaac aatttaataa
     9241 atataaagaa cagattattt caagtgaaaa agtaactgaa gatataattc caagtatata
     9301 tgagtatggg gtttatactt tggatgggaa tttaatttca gggaccttta ataaaaaaga
     9361 atcaaaagaa gtatggaatt taatgagaga tattgaagaa aggcatgcat attcagaaag
     9421 ttatataaag ttttttaaga aagatgaagt ttttataata aaatacaaaa tagtatcaga
     9481 gtatagttct ccaattttaa gagcatattt accaaaacca gagacattgg gtatgattat
     9541 ttttagtata atatttttta ttgaaattgt tatattatca aaagtatttg gcaagaaatt
     9601 taatattgaa atggagcttt taaaaaatac tacagaaaaa atagaacaac aggatttaga
     9661 ttttgttgta gaatctagta aaatttatga aattaataat gtcctttttt ctatggataa
     9721 aatgaagttg gcgttaaaag attctttaga aaaacaatgg atgctagaag aaaatagaaa
     9781 ggaacagata tctgcactgg cacatgatat aaaaacacca ctaacaatta tacatggaaa
     9841 tactgactta ttaattgaaa taaatgaaaa tcctgagtta ttagaataca tggaatatat
     9901 ctcaaagggt gtcactcaaa tagaaaaata tattaatact ttaatagata tatcaaaaac
     9961 agagaatggc tatatattaa ataaagaagc aatcagtatg agtgagttta taaaagatgt
    10021 tttagtgcaa atagaagcat tagctagtac taaaaactta gatgtggagt tttataaaca
    10081 agataatttg cctaaaaata taactataga tagagaactg ttattcagag ctattatgaa
    10141 tataatttca aatgctatag attattcacc aagccagagt aaactataca taagtgtatc
    10201 agtacataat aaatacttaa aatttgtagt tacagattgt ggaagtggtt tttctaaagc
    10261 agatttgtca aaagctacag aacagtttta tacaggagat ttgagtagaa attctaaatc
    10321 tcattatgga atgggactct atattgtaaa taatattgta caaaaacata atgggacttt
    10381 atgtattgaa aattctgtga aaactggtgg tgctatggta acaattgaaa taccaatagt
    10441 ttagtaaata atatccattt tgtaaatacc taaattttat gatttatagg tatttataaa
    10501 atggatagtt tttttgattt tatatattgg ttaaatctga taaaaagttt tcaaacataa
    10561 ctccatcatt tctatcagta tcaaattgag atgtgtaatt tatagtttta tatataaaat
    10621 ctgttgcagt attaaccgct actcccaaat catgtttatt tactaacata ccacaaagta
    10681 tagatgtgaa tatatctcca gttccactgt aagaacaatt attatatttg actgatgtaa
    10741 agaatacatg gtcattatct ctattgtaag ccaaatttaa tatattatca tcttctaata
    10801 taccagtaat aacgactttg ctaggtccca aatcagatac agattttgca atatatataa
    10861 gctcatctct attataatcg cttttggtat aatcatttcc agttaagaaa caagcttctg
    10921 ttaaattagg agtagttaaa tctgaatgtt taactaattc tttaatttcc tgtctcattt
    10981 cttctgtgaa tattggatac ataactccat tatcacccat cacagggtca actacgataa
    11041 aagcattggg ataatcgttt ataaaattag ccactatctc aatttgatgc tttgaaccca
    11101 aaaaaccact atatatacta tcaaaattga cttttaaatt tttccaaaca tttgaatatt
    11161 ttaccatttc atcagtaaag tctaagaaag taaattcagg ataaccagtt tgacttgaaa
    11221 gtatggccgt tggaaatgga caacattgaa cctttaatgc tgaaagtata ggtatagcaa
    11281 ccgataatga gcattttcct attccagata agtcattaat tgctgcgact tttttttgca
    11341 taaaaaaccc tccattagta tgtattaatt ataatgatac caaaatattt atatacatat
    11401 atattactat aaaaaaaaga aaaaagtaga ttataaaata aaaaattgta aaaaaaacag
    11461 tgtattgata caattaattt ttaatattgg acataataaa atagagaatt attaaatatg
    11521 aaaattacta tatgtgacag aatattcttt gtaatttgat ttgtaaggtg tactatttgg
    11581 gaggtgtagc tttgaaaata ctgaaaaata agaataagta catcaaaggt ataatggcat
    11641 ttgtattttt gtctttagct actacagcag gtgtattttt aatcaatgat ttaagtatga
    11701 aaaatatgga agaaaaagtc atggaatctg caaatgttga aggtgatgat gtaaaagacc
    11761 aatataaact attgcttgaa aacttatttg actacagaaa taaggcaata ttagagaaaa
    11821 atgaaaatat tttaaaaggt ctttatgaaa cggataaaaa atttggtctg tgggcttatg
    11881 aaaatgaagt aaaaaagatg aaatatttag aaaactggtc tagtaaacaa ggtgtaaaat
    11941 ttaaagacat aaagacaagg gttaagatta aaaaaataaa ggaaaaagaa aaagacctct
    12001 atggtataat ctgttcagta tccactgaat ataagtattc atatgaaaat caaaaggaca
    12061 aagaaaatgc ctttagaata ggtacatatc attatctaaa tgtaaaaatt atagataatc
    12121 aatatgtcat aactaaagag tggtatacag acccttttgc agactcttta aatttagaaa
    12181 atataaaatc tgatgatata agaaattata ttggtcaaca agatggtgta gagttacagc
    12241 ttacagatga acaaaaaaaa gctatagatt atgcacataa atactgtgga gccgcagctg
    12301 atgaagaaca tggtatgaaa tttaatccaa actataggga ttataatcca gaaggtggag
    12361 attgtgcaaa ctttgcatca cagataatgt ttgaaagtgg aaggttcaaa aagaactcaa
    12421 tttggaatta tagcgagaga gcaggtacaa aagcatgggt aaatgcacaa gcttttaaaa
    12481 attatgcact gtatagtgga agaggttcat taattgctaa aggttcctat gaagaagtat
    12541 acaaagaagc ttataattta cgtccagggg attttgttgg ttatgagaag aaaggcagaa
    12601 taacacatgt ttcgacagtg acaggattag actcaagagg atatcctttg gtaacttgcc
    12661 ataatactga tagaatgttg gtgccatggg atttaggctg gagtgataag tccataagat
    12721 tccatataat aaaaataaat tattaaaaaa ttataaagaa aaataaagtg taaaagataa
    12781 taatcattta aaattataaa gtaaaaaaat tgattattaa tgtataaaaa taataataat
    12841 tgacaaacat agctatatat gatagagtag taataaggtt acagaaaaga gtagctaaat
    12901 atttgtgttt ttttaggagg atttattaat gaaaactggt gtagttaaat ggtttaacaa
    12961 cgaaaaagga tttggattca tatctgtaga aggtgaagga gatgttttcg tacatttctc
    13021 agctataact ggagatggat acaaaacatt agaagaagga caaagcgttg agttcgaagt
    13081 agtagatgga gctaaaggtc ctcaagctgc taacgtagtt agattataat taaaaattca
    13141 tatatattag tgttctagta atatgagaga taagccgcta atcatttagc ggttttttca
    13201 gtttttgtaa ataaaattaa tagcaaaata aattaaaatt attaaaataa aatcattaat
    13261 aatgagataa atatgttaaa attaatagtg tagagtaaaa ttattatggc taagtttaag
    13321 tcaattaatt tttagtaaat tttctaaaaa gagaataagt gatacttata gaccatataa
    13381 aaatattaat aaagcataat aagattaaaa tgagcaaagg aaaaacgctt gttacagcgt
    13441 atagtattaa gaatacttag aaaataagaa aggagaaagt tactgcaaaa aagtaacatt
    13501 ataaatattg agtaatattt ataagttttc agtaacggtg ggtagtgtaa tgaacatatt
    13561 attctttcta acaccaaaaa gtgaagtagc atatatatat gaagactaca ctataaggca
    13621 agccctagaa aaaatggaat atcacaaata ctctgcaatt ccaataataa gtaaggatgg
    13681 taaatatgtt ggaactataa cagaaggtga ttttttatgg acattaaaaa atgatttaaa
    13741 tctggactta aagggattgg aagatgttcc agtgacagat ataaatagaa aaatggataa
    13801 ttctcctgtt tccatcaacg cagatattga agatttagtt ataaaatctt taaatcaaaa
    13861 tttcattcct gttatagatg accaagacac atttattggg ataataaaaa gaagagatgt
    13921 aataggttac tgttatgaga taatacgtgg atataaaaat ttagctaatg ataattaaaa
    13981 atcttattta aacttttaag ctgagttctt tacaataaaa aaatgataat atgaaactga
    14041 atatttaaac tttaatataa aaagctgtct tttctaataa atattgtgaa aagacagctt
    14101 ttttatatat gaccttatga ttaatcgttg tattttacaa ggtttccgta cttagcatta
    14161 agagcattta tcttatttcc ataaacttca ttttgttttt gatataataa acttttttca
    14221 acttcagcct taacttcatc aaatgtagac tctgtacttt cttgaaggtc ttctaatttt
    14281 attatatggt atccaaattg agttttaact ggttcactta cttcaccttt agccatagag
    14341 aacacagctt cttcaaattc aggaaccatt tgtcctctac cgaatgttcc taaatcacca
    14401 cccatatctt tagatgggca agaagaatgt ttaagagctg catcttcaaa agatatttct
    14461 tctgatttta tttgagcaag tatttcttta gctttttcat cactatctac taatatatgt
    14521 ttagcagttg cactttctgg tttagagaaa gagctcttat tagcttcaaa gaattttact
    14581 ttttcatctt ctgttacatt aacactagat aaaactttat ttatagcaaa ttgctttaat
    14641 acattttctt ctatcttttt catttctaat ttaaatattt cttcttcatc taatttttct
    14701 tctttagcat ctaagaagaa taattcttga ttcactaaat catttaataa gtgttttttt
    14761 ccttcttcag ttttaaattg catagcttga taagggtcta atgattttaa tgcattttca
    14821 atatcaatat tagttatctc tttttctcca actgttgcta aaactttttt ttccataata
    14881 gttaactcct tcatagtgta tttcattatt aatataagac taccatctta aataatatca
    14941 gaaaataatg tagatgtcta atatttaaag ccatattaag tcaaatagaa ttaaatttag
    15001 tagaacaaag ttattacaat taataaaagt gagctcataa tacccactac accttcgtca
    15061 tctttaaatt taagagtttt tactattgaa taataaaggt atattccagt aaaaataatc
    15121 tttaaataca agaaaaataa actctttggg tatattaaaa agctaaaaag tgtaaatata
    15181 aaagcaagta gagtacttat cctgtaaaag aaatctattt gtgtaatgtc tttatatttt
    15241 atcataaaca ccgaatataa aatgatgaaa acaattgcga cgattgcata tatggtaaat
    15301 aacatatttt caagagtacc atttgaaatt actatccact tataccacat aattggccaa
    15361 aataatagtg ctaagaactt aaaataatta tcaaacaact tttctttata cattcatcaa
    15421 acaacctttc ttaacaaaag catatatttg tttttaga
//
LOCUS       DAYASV010000090        15340 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYASV010000090 DAYASV010000000
VERSION     DAYASV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15340
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.091"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            43..1494
                     /locus_tag="ACGI68_002650"
     CDS             43..1494
                     /locus_tag="ACGI68_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0139078.1"
                     /translation="MDFFNGILTSINNAVVYVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGINKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1713..2174)
                     /gene="ribE"
                     /locus_tag="ACGI68_002651"
     CDS             complement(1713..2174)
                     /gene="ribE"
                     /locus_tag="ACGI68_002651"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFH0139079.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            complement(2279..3472)
                     /locus_tag="ACGI68_002652"
     CDS             complement(2279..3472)
                     /locus_tag="ACGI68_002652"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFH0139080.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(3495..4160)
                     /locus_tag="ACGI68_002653"
     CDS             complement(3495..4160)
                     /locus_tag="ACGI68_002653"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFH0139081.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSNNHKSNIDKDFLFK
                     NGF"
     gene            complement(4194..5306)
                     /gene="ribD"
                     /locus_tag="ACGI68_002654"
     CDS             complement(4194..5306)
                     /gene="ribD"
                     /locus_tag="ACGI68_002654"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFH0139082.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKASNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(5438..5559)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            5748..8231
                     /gene="recQ"
                     /locus_tag="ACGI68_002655"
     CDS             5748..8231
                     /gene="recQ"
                     /locus_tag="ACGI68_002655"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFH0139083.1"
                     /translation="MNKKLLEILSKYYGYTSFRKGQESIINSILSEKDVLAIMPTGGG
                     KSICYQIPALILDGMTIVISPLISLMKDQVDALKTMGISAAFINSSLSSKEFNEILNN
                     IRKNNYKILYIAPERLDAQEFIELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDF
                     IKNLPKRPIVTAFTATASEEVRTDIINLLCLENPDYYITGFDRENLSINIVKSSSKNK
                     YILDYIQNHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFI
                     NDNVDIMVATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFS
                     PGDVHIQKYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILSYFGENYIDNCN
                     NCSNCLVEGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLS
                     TYNIMRDYSSEDLKNFVNTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVK
                     VEFKEIVMTKSLEVEDELYNALRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKE
                     EMLDISGVGEIKYQKYGRDFEAVIKEYVEKNNINKKIDEKNVDSKNVNSDFLEVNTDK
                     VLYEKLLVIREEYATKEKLPAYMILANKALKEISGRYPLDEEQLKDISGIGGVKFEKY
                     GASILAEVNKYVKENNINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQE
                     IEVSLSTIIGYVHDYVKDGHSISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRK
                     LPDSIKYENIRAVILDSYLENLKNNIIAE"
     regulatory      8320..8431
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8573..9874
                     /gene="thiC"
                     /locus_tag="ACGI68_002656"
     CDS             8573..9874
                     /gene="thiC"
                     /locus_tag="ACGI68_002656"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFH0139084.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            9928..10122
                     /gene="thiS"
                     /locus_tag="ACGI68_002657"
     CDS             9928..10122
                     /gene="thiS"
                     /locus_tag="ACGI68_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFH0139085.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            10115..10918
                     /gene="thiF"
                     /locus_tag="ACGI68_002658"
     CDS             10115..10918
                     /gene="thiF"
                     /locus_tag="ACGI68_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HFH0139086.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            10981..11751
                     /locus_tag="ACGI68_002659"
     CDS             10981..11751
                     /locus_tag="ACGI68_002659"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFH0139087.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            11768..12871
                     /gene="thiH"
                     /locus_tag="ACGI68_002660"
     CDS             11768..12871
                     /gene="thiH"
                     /locus_tag="ACGI68_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFH0139088.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEVISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            12872..13561
                     /locus_tag="ACGI68_002661"
     CDS             12872..13561
                     /locus_tag="ACGI68_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFH0139089.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKEFRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            13749..15143
                     /locus_tag="ACGI68_002662"
     CDS             13749..15143
                     /locus_tag="ACGI68_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFH0139090.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
BASE COUNT         5678 a         1832 c         2616 g         5214 t
ORIGIN      
        1 tttattgaga aaacaaaact aaataggggg gtaattaata taatggattt tttcaatggt
       61 attttaacga gtattaacaa tgcagtagta tatgtaaaca attacttatg gtcgtatatc
      121 ttaattataa tgttagttgt aattggtatt tactttacta ttaaaacaaa ttttgtacaa
      181 ttcagatatt ttatagaaat gtttagactt ctgggtgatg gtacagcgaa taaaaatgca
      241 aaaaaggaag gaaaaatctc ttcatttcaa gcattttgta taagtacatc ttcaagagtt
      301 ggaactggaa atatagctgg aatagcaatt gcagtggtag caggaggacc tggtgctgta
      361 ttttggatgt ggcttatagc attaattggt tctgcatcaa gctttgttga gagtacatta
      421 gctcagattt acaaagtgaa agatggtcaa gcttttagag gtggaccagc gtattatatg
      481 gaacaaggtc taaataaaaa atggatgggt attttattct caatacttat aactatatgt
      541 tatggttttg tatttaatgc agttcaagca aatactgttt cacttgcatt taataatgca
      601 tttggaataa ataaaatggc tatgggaata ggtcttggaa ttttaactgc aatagttatt
      661 tttggtggag tgcatagagt tgcaaaagtt tctgaaataa tagttcctgt atttgcagga
      721 ttatatatat tagttgcttt ggctatagtt gtaatgaata taacagagat accaagtgtt
      781 atagcattga tagttgaaag tgcatttgat tttaaaggaa tggcgattgg tacatttatg
      841 ggagttgtaa tgactggagt gaagagaggt ctattttcta atgaagcagg tatgggtagt
      901 gctcctaatg ctgctgcaac agcacatgta actcatcctg taaaacaagg tttaattcaa
      961 tctttaggtg tttttacaga tactataata atatgtagtt gtacagcatt catggtatta
     1021 ttatattcag gttatagtag tagtggagca actggaattg aattagctca agaagcttta
     1081 actcatcata taggaccagt tggaaatata tttatagcag tttgtatatt cttgtttgca
     1141 tttagttcta tagttggtaa ttactattat ggtgaatcaa atatggagtt tatgagtgga
     1201 agtaagacta aacttaatgt atttagagtt tttgttgtag gtatggtttt atttggttca
     1261 cttactcaag tggatgtagt ttggaatctt gcagacttat ttatggcatt gatggcgata
     1321 ataaacttaa tagcaatagc acttttagga aaatatgcat ttattgcttt aaaagattat
     1381 acaagtcaaa aaaaatcagg aataaaagac cctgtgttta ttgcagatga aatagaaggc
     1441 ctagaaaagg tatcagagtg gcatagggaa ttatctgagg aacatttagg ataaaaaata
     1501 gtataaaatt ataaaaccat tttgaaagac tagagtatgt atatacttta tatgaatata
     1561 atattgaatt aatttatatt actggaattt ttaattccag taatattttt ttattatata
     1621 atttagcttt attaaaagtt tttattatta aaaatagctt atttatcgta aagtgaatga
     1681 tattgggttt taattattat gaatgttagt atttaattta aagacttaaa taaatttgcc
     1741 atttctagcg cattcatagc acaatcataa cctttatttc cagcttttgt acctgctctt
     1801 tcaatagctt gttcaatatt ttctgtagtt acgattccaa atataacagg taattcactg
     1861 tctaaagata ctttggctat ccctttagag acttcactac atacataatc ataatgagaa
     1921 gtagcccctc ttataacaca gcctaaacag attatagcat catacttacc acttttagcc
     1981 attttttgag caacaagagg aatctcaaaa gctccaggta cccaaactat ttctatattt
     2041 tctggagaaa cgtcatgtct taaaagacag tcttcagctc ctgataaaag ttttgatgtt
     2101 ataaattcat taaaacgaga atttattata cctattttta aatcttttcc aataagtttt
     2161 ccttcatata tcatttttac ttcctccaat tttatttatg ttatagattt atatttataa
     2221 atatctttaa tttaattata aaaaactact tagtagaaaa atatcatcat ataatcattt
     2281 atataatatc taaaagatgt cccattttat cttttttagt ttttaaatat agcttattaa
     2341 agtcattaat ttcttcatta atagggattc tattttcgat ttttattcca tatttctcta
     2401 attgatttat tttatctggg ttattactta aaagatttat actttttatg tttaaatttt
     2461 ttaatatttg agcagccata tagaaatctc tcatatcttc ttcaaatcca agcattaaat
     2521 ttgcttcaac agtatcgtaa ccttcttctt gaagtttata agccttaatt ttatttaaaa
     2581 gaccaatacc tctaccttct tgcttcatat aaattaaaac tccacttcca ttttccacaa
     2641 ttttcttcat agaagattct agctgtaatc cacaatcaca ttttagagag tggaacacat
     2701 ctcctgttaa acattcagaa tgaagtctaa ctagagtatt ttctatattt atatttccat
     2761 aagtaagggc aatgtgttct tcatttgaat aagtatctct atatcctatg atttcaaaat
     2821 ttccatattt tgtcggaagg aaagcactag atactttttc tatagtagtt tcatttattt
     2881 tgcggtattc tataaggtct tttatagtga tgatttttaa attatgcttt tctttgaaaa
     2941 tcattaaatc atcaactctt gccattgtac catcatcttt cattatttca cagataagac
     3001 caatctcttt aaaacctgca agtctagcaa ggtctactga ggcttctgta tgtccatccc
     3061 tgacaagaac cccaccagtt tttgctatta aaggaaagat gtgaccaggc gttttgaaat
     3121 ctttacttgt agaattttca tcacagaata attttacagt ttcagctctt tcatatgcag
     3181 atataccagt tagagtatct gctccatcta tagtaacagt aaaggctgtt tgaaatgtat
     3241 ctgtattttc tctaaccata agaggtatat taagactttt aaattttttt tcagtggcag
     3301 gtagacaaat tagacctcta ccatatgtag ccataaaatt tattgattca ggagtagcca
     3361 tttcagctgc catcaataaa tctccttcat tctctctact ttcatcatca accacgatta
     3421 ccattttccc attttttata tcttcaatag cttcttcaat agtattaaac atgtaattca
     3481 ctcctttatt atttttaaaa accattttta aataaaaaat ctttatctat atttgattta
     3541 tggttattac tattagaatt taattccttg tagttattag ccataaaatt attaacatac
     3601 tttccaatta catcgctttc taaattaacg aaatctccaa cattctttgt caagagaata
     3661 gtgttaattt tagtatgagg tataatagaa accttaaata tttcatcatc taaatatgat
     3721 atagttaaac ttacaccatc aatagcaaca gaacctttga gtatcattga ggataaaagt
     3781 tttttatcag gtcgtatact tactaaaact gcattttcat ctttttcaaa tttgcagata
     3841 gttcctttac catcaacatg acctgttact atatgaccac caaatcttga agaaagactc
     3901 atagctcttt caagatttac cttgtcattt gcatttaata aacctaaatt tgtggaacga
     3961 attgtctcca tcataacatc ggctgtaaat tcattttttc cgaggtttga aacagttaga
     4021 caaactccat ttacggctat actatctcca agatttgtgc catctaatat tttatttgat
     4081 agtatggtta cttttccact cttaccatta gcagtaattt ttctaagtat tccaacttct
     4141 tctactatgc cagtaaacat cctaacctcc tatatgaatg tattaacaaa aatttaattt
     4201 aaaacatctg attctataag aatatcgcca tctatcaaag ttacattatt gattgttaac
     4261 tgtgttgaat ctgacaaatg atttattcct tctccagaaa cgaatgtagg tgcatcttta
     4321 ccacctataa tcttgggtga tagaaataat ttgaccttat caactaactt atttctaaag
     4381 agactgtcat tgagagtagc accaccttca actagaacag aatcaatgtt taactctcct
     4441 aatttataca ttacaaactc caaatctact aagttgtttt ttgaaggaga aattattgtt
     4501 tcaacattat tttcttttaa catagataat tttatacttt ctttgcaaca tgtgaaaact
     4561 atagttttac tggttttatc tttaaccaaa ttactattaa gtggcaactt taggtgtgta
     4621 tctattacta ttcttgtagg atgagacact ttttcttgat tgagcctaca agttagtaaa
     4681 ggattatcgt tcaaaactgt atttatgcca accattatac tttggtattt atttctgtat
     4741 ttatgagtta gataccttga tgattcatta gatatccatt ttgattctag tgattttgta
     4801 gcaattttac catctagact tactgctgat tttacaatac aaagaggtct tttgtttttt
     4861 atatgataaa aaaatgcctc atttaaatct cttgcttcat cttctaatat tcccacagta
     4921 acatcaatat tgttatcttt taattttttt actccattac cacaaacaag tggatttggg
     4981 tctaatgtag atattacaac tctcttaatc ttattttgta taattttatc tacacaagga
     5041 ggcgttttcc cataatgaga acatggttct aagtttacat aaagagtaga gtcttttaga
     5101 ctttgtttag cactgtttat agcatttact tcagcatgat tagagccaaa tttctcatga
     5161 tatcctttgc ctattatatt actagcttta acaattacac acccaactaa tggatttgga
     5221 tttacaaagc cttctccatt tttagccaat tcaatagctt ttttcatata ataaatatct
     5281 ttttcttttt gattcacaaa attcacaata tgtacacctc ctgtttaatt ggataatttt
     5341 taaacaaaaa accctgagaa ttaactcagg gtttagaata atctacaata aatgtttaat
     5401 aaatgtagaa caaaattgct ctacaaaaac actcattaaa tatcttcttc catccagact
     5461 ttactgtcgg ctttggaatt taaccaaatc aaccaagttt taccttggct cgcgggctat
     5521 accgccgatt gggaatttca ccctaccccg aagattaagt attaaattta tatttagaat
     5581 gtatcataaa ttattttact ttgcaaacta tttttttaaa aaatatatgt taatatataa
     5641 tatagaatgt ctataaactt tgaaatacca cgattgtgaa tataaaaata aaaatttgaa
     5701 cataatcagg aaagaagtct aattaattta aattaggaga gtaatatatg aataaaaaac
     5761 ttttagaaat actatcaaaa tactatggat atacaagttt tagaaaaggt caagaatcta
     5821 taataaactc tatcttaagc gaaaaagatg tgttagctat tatgccaaca ggagggggaa
     5881 aatcaatatg ttatcaaatt ccagctttga ttttagatgg aatgacaata gtaatttctc
     5941 cacttatatc acttatgaaa gaccaagtag atgccttaaa gactatggga attagtgctg
     6001 cttttataaa tagttcatta agtagcaaag aatttaatga aatattaaat aatattagaa
     6061 aaaataatta taaaatactc tatattgctc cagagagatt agatgcacaa gagtttattg
     6121 agctaataaa taataacaat gtaagtcaag ttgcgataga tgaagcacat tgtgtatctc
     6181 aatgggggca tgattttagg ttgagttata gaaggatttc tgattttata aaaaatttgc
     6241 ctaaaagacc tatagttaca gcatttactg caacagcttc agaagaggta agaactgata
     6301 taataaattt actgtgttta gaaaatccag attattatat aactggattt gatagagaga
     6361 atttatcaat aaatatagta aaatcatcta gtaaaaataa gtatatttta gattatattc
     6421 aaaatcataa aaatgagtct ggaattatat atgttgcaac tagaaaggaa gttgaaaata
     6481 tatataatgg tttatctaag agaaatatat ctgtgtcaaa atatcatgca ggtttatcta
     6541 aaaatgaaag aagtaaaaat caagaagatt ttataaatga taatgtggat ataatggtag
     6601 caacaaatgc atttggaatg ggtattgata aacctaatat aagatgggtt atacattaca
     6661 atatgcctca aagtatagaa aactactatc aagaaatagg tagagctggt agggatggag
     6721 aaaaaagtga gtgtgtactt ttattttctc caggagatgt acatatacaa aaatatttaa
     6781 ttgatattgg agttgaaaat cctgaaagaa aattatttca gcataagaag cttcaataca
     6841 tgattgactt agtttacagt aattcatgct atagaaagac aatattgagt tattttggag
     6901 aaaattatat agacaattgc aataattgta gcaattgttt agtagagggt gaaatagtag
     6961 ataaaactat agatgcacaa aaagtcattt catgtgtagc tagaatgaaa agaagttatg
     7021 gtgttactac tattgtagat gtgcttagag gttctaagaa taaaaagatt ttacagcttg
     7081 gattaaatac tttgtctaca tataacatta tgagagatta ttctagtgaa gacttaaaga
     7141 attttgtaaa tacattggtt tctcatggtt ttttagattt agttgagact ctaggcaatg
     7201 gaaatagagg tagttttcct actataagat taaatgagat gtctatgaaa gttttaaaag
     7261 gtgaagtgaa agtagagttt aaagaaattg ttatgacaaa atcattggaa gtagaggatg
     7321 agttatataa tgctttaaga gaacttagac attcaattgc aagtgaagaa aggatagctc
     7381 catatatggt atttggagat ggtacactta gagccatgtc tagtagttat ccaattaata
     7441 aagaagaaat gctagatatc tctggagtag gagaaattaa atatcaaaaa tatggtagag
     7501 attttgaagc agtaattaaa gaatatgtcg aaaaaaataa tataaataaa aaaatagatg
     7561 aaaaaaatgt cgattcaaag aacgttaata gtgatttctt agaggttaat acagataaag
     7621 ttttatatga aaaactgtta gtcattagag aagaatatgc aacaaaggaa aaattacctg
     7681 catatatgat tttagccaat aaggctttga aagaaataag tggaagatat ccacttgatg
     7741 aagaacaatt aaaagatatt tctggcatag gtggagtaaa atttgaaaaa tatggagcta
     7801 gtattttggc agaggttaat aaatatgtaa aagaaaataa tattaatgtt atttgggaga
     7861 aaaagggcaa aaggaagtta atattagatg gagaaagtag aaaaaataat gaaatagcat
     7921 tagatttgct aaatcaagga aaagatatac aaagtatcag tcaagaaata gaagtttcac
     7981 tgtcaaccat tattggatat gtgcatgact atgtaaagga tggacatagt atatcatttg
     8041 aattaggact agaaaatttt tataatgaag ataaaaagaa gatgatttta gaagtttgcg
     8101 aaaaagttgg ctatgataat ttaaataaca taaaaagaaa acttccagat tcaataaagt
     8161 atgaaaatat aagagctgtg atacttgact cttatcttga aaacttaaaa aataatatta
     8221 tagcagagta atttttaact taataaaaaa taaaatttgt attattgtgt tgaactaata
     8281 attttgctgt gttatactga atttgtaaaa ataatgaaaa attagagcta ggggcgctag
     8341 aattggctag ctgagagata aagccgtaac ttacaaaccc taataacctg atcaagataa
     8401 tgcttgcgaa gggaagcttt ttaatataat tttattatat aaataggcaa attattcaat
     8461 gttaggagaa atttgtctaa gtgtatatac gattataaga gtgctttcca aacgaggaaa
     8521 agcacttttt ttattttcaa aaactataac tttaatcttg gaggattgta atatgaacta
     8581 tacaacacaa atggatgctg ctagaaaagg cataataact aaagaaatgg aaatcgtttc
     8641 tcaaaaagaa caggtagatg taaatgaatt aagagaatta atagcaaatg gacaagttgt
     8701 tataccagct aataaaaatc ataaatcttt aagtgcagaa ggtgtaggta aaaatttaag
     8761 aacaaaaata aatgtaaatc taggaatttc aagagattgt aaggatatag aaaaagaact
     8821 tgaaaaagtt agagtagcaa tagacatgaa agctgaagct ataatggatt taagtaatta
     8881 tggcaaaact agagaattta gagaaaaggt tgttgaaatg tcacctgcaa tgataggttc
     8941 agtacctatg tatgatgcag taggatattt ggaaaaagaa ttaaaagata taacagaaga
     9001 agagttttta aatgtaataa gacaacatgc tattgatgga gtagacttta ttactataca
     9061 tgcaggtctt acaagaagtg tttgccaaaa aataaaaaat catgagagat tgactcatat
     9121 agtatctcgt ggaggttctt tattgtttgc atggatggaa ttaaataata aggaaaatcc
     9181 tatttatact aattttgata aaatattgga tatatgtgaa gaatatgatg ttacattgag
     9241 tttaggagat gcttgtagac caggttgtat aaaagattct actgatggtg ttcaaattca
     9301 agaacttgta gtattaggtg aacttactaa aagagcatgg gaaagaaatg tacaagtaat
     9361 gatagaaggt cctggacaca tggctattga tgaaatagaa gcaaatgtag tattagaaaa
     9421 gagattatgc catggagcac ctttttatgt tcttggacca ttggttacag atatagcacc
     9481 aggttatgac catataacaa gtgcaatagg aggagcttta gcatgtgcca aaggagtaga
     9541 ttttttatgt tatgtaactc cagcagaaca tttaagattg cctaatttag atgatatgaa
     9601 agagggtata atagctgcaa agatagctgc tcatgcagga gatatagcta aaaatgttaa
     9661 aggtgctcgt gaatgggata ataagatgag taaagctaga gctgatttag actggtgtga
     9721 gatgtttaga cttgcaatag accctgaaaa agctaagaga tacagagatg aatcaactcc
     9781 tactcatgag gatagttgta ctatgtgtgg aaaaatgtgc tcaatgagaa cagttaagaa
     9841 aatattaaac aatgaggaac ttaatctaat ataatttttg gctagtatga ctggaataaa
     9901 aataagctga aaagaggagg tgatttagtg aaggttaatg ggaaggaaat tgaatttgaa
     9961 aaagatttga ctgttataga tttattaaat aaatataatt taaaaagtga tagagttgta
    10021 gttgaagtga atttagagat aattgaagaa tctaactaca atacatatgt attaaaagac
    10081 gaggacattg tagagcttat tagctttatt ggaggtggtt aaaataaaaa tatttgtaaa
    10141 tgaattatta acagatgttg aggaaaatac aactgcttat aaagttagag atagctataa
    10201 caataaatgt gatgtattgg tgttaaatgg atatccaatt aaaacagata tgaccctttg
    10261 tgaaaatgat aaattaacac ttatacaaaa aggagttaag ccatcacttg atgaattgga
    10321 aagcttgttg attgctagac atactccaaa tatacataat aaattaaaaa aagggaaagt
    10381 tgctatattg ggattggggg gacttggttc caatatagct atctctttag ctagaatagg
    10441 agtgggagaa cttttactta tagattatga tgttgtagaa ccatctaatt taaatagaca
    10501 gcagtatttt atagatgata taggaaaact aaaaactgat gccatgatag aaaatataaa
    10561 aaaaataaat ccatttataa aattaaataa acgagatatt tttttaaaca agaacaatat
    10621 ggatactatt aaggattcag atttaataat tgaggccttt gatgatgctt catgtaaagc
    10681 acaggtatgt aattatgtat taataaattt aaaagataaa tatttaatag catcatctgg
    10741 tatggcaggt tactatgatt caaatataat aactactaag aagataaaag acaggtttta
    10801 tatatgtggg gattttgtga atgaagctaa atttggtgaa gggcttatgg ctccaagggt
    10861 tgccatctgc gcaaatcata tggcaaattt ggcaacgcaa atacttatag atatgtaaat
    10921 attaaaatta gatataaaaa agattgaata ttaaaaaaat ttaacgtgga gggaaataat
    10981 atggataaat tggtacttgg tggacatgaa tttaatagca gattattggt tggaacaggt
    11041 aaatatggtt caaataatat attgcctgag gtaataaaag aaagtggaag tgaaataata
    11101 acaatggcac ttagaagagt ggatttagac aataaacaag aaaacatatt aacgtatatt
    11161 ccaaaagaga tgacaatact tccaaataca tcaggagcta ctaatgcaga agaagctgta
    11221 agaatagcta gaatatctag aaaaatggga tgtggagatt ttataaaaat agaagtaata
    11281 tcagatacta gatatttact tcctgataat gaagaaacta taaaagctac aaagatactt
    11341 gctgatgagg ggtttatagt tcttccatat atgacaccag acctttatgc tggaagaaga
    11401 cttatagaag ctaatgcagc ggcagttatg ccacttggag ctccaattgg ttcaaataga
    11461 ggtcttcaaa tgaaagaaat gataagaata atgatagatg agttggatat accaataata
    11521 gtagatgcag gtattggaaa accatctcaa gctatggagg ctatggaaat gggagctgat
    11581 gcagtacttg tcaacactgc tatagcttca gcaggagacc ctgtacaaat ggctagagca
    11641 tttaaattag ctgtagaagg tggaagagaa gcatatattg caaaaacagg aaatgtgtca
    11701 gaatttgcaa atgcatcttc tcctttaaca ggttttttag gcaatttata gaaagtggtg
    11761 attggtgatg agtttttatg atgtaataga aaaatatagg gattttgatt ttgatggata
    11821 tttaaataat gttactgata atgatgtttt aagaagttta tcaaaagaca agcttgaaga
    11881 ttttgatata ttaaatttgc tttctaaaac tgcagtaaaa catttagaag atatggcaca
    11941 aaaagcacat aaattaagtg ttcaatactt tggtaaaaca gtatgtttat atacaccaat
    12001 gtatatagca aattattgtg ttaatcaatg tgtttattgt agctataata taaagagtgg
    12061 aataaaaaga aaaaaattaa ctatggatga aattagagaa gagggagaag taatttcaaa
    12121 agaaggtttc aaacatctac ttgttttgac tggagaaagt agttttcact caagtgtaga
    12181 atatataggt gaggctatag aaatattaag agagaaattt ccctctatag gtatagaagt
    12241 ttatccaatg gaagtagaag aatataagta cattgtagat aaaggtgttg aaggattaac
    12301 agtatatcaa gaaacttatg acgaggaaat atataagaga gtacatataa aaggcccaaa
    12361 atcaaattat aaatttagac tagatgctcc tgaaagaggg gcaaaagcag gtatgaggac
    12421 tttatcaata ggtgcacttt tagggctaaa tgattttaga aaagaaactt tttttacaat
    12481 attgcatggg aaatacttaa aaacgaaata tcctcatatt gagctttcat attcaacacc
    12541 aagaatgaga ccttttaaag ggtgttttga ggaattggta gatataagtg atactgattt
    12601 agttcaagct atggtatgta tgagattatt tgaccctcat gcagctataa atatatccac
    12661 tagagaaaat ttagaaatga gaagtcatat aataccttta ggagtaacta aattgagtgc
    12721 aggtgtatct actgatgtag gaggtcattc tcaagatgaa catgatactg ctcaatttaa
    12781 gataaatgat gaaagtactg ttaaagatgt ggagaaaatg ctaaactcaa ttggttatca
    12841 gcatgtgttt aaagattggg aaagatttta aatgtatctg ataacaaaca gaaaattatg
    12901 tagtgaggaa cgatatttag aagttattaa agagtctata ttgagtggtg tagaaaatat
    12961 aataataaga gaaaaggatt tagaatatca agagctaaga aaattatata tgaaaataaa
    13021 aactaaaatc aattgtatag attttcaaga gcaaatatca gatgaaagtt tgaaaactaa
    13081 tataaatcaa aaagagttta gaaataaatt caaggttaat tttataataa atagtaacat
    13141 tgaatttttt gaaaaagtgg attgtcaagg tatccatctt ccttttaaat tattcttgaa
    13201 tttaattgaa aataagtata attttaatga aaataaaata ctaggattat ctttacataa
    13261 agtagaggaa gtagattatt tagaaaaatt aataagaaat caaaatataa aaatagatta
    13321 cataacatta tcacatatat atgaaactaa atgtaaagaa ggattaaatc caaaaggaat
    13381 tgagctttta aaagaagcta aaaagattac tgatattaaa ataattgcac ttgggggaat
    13441 tttaccatct aatgtcaagg aaacattaaa atactgtgat gattttgcta taatgtcaac
    13501 tataatgagg tctaaagata ttaaaaaaac catatcaaat tataatgaaa agttgaatta
    13561 gtaaatatag tatattgaat ttttaactta aataaaaata aaaaagttat cttttaaatt
    13621 ttaaaaggta gcttttttat tttatatctt tgctaataaa aaattgtaaa aagggaaaac
    13681 tttagtcaga gagattaaac aatatcctag aaagatttaa aatttagtaa agtatggagg
    13741 acattataat gactgacagt aaaaagaaac gatttattat accacacact tttacaataa
    13801 tatttttact aatagtcttg atggcaatat tgacttggat agtaccaagt ggtgaattta
    13861 gtagagaaga tgtagaaggt agaatggtag ttgtaccagg gacttatcaa agtgtagaaa
    13921 gtaatcctca aggatttgca gatgttttta aagcaccaat agaaggcttt atcgattcag
    13981 ctgaagttgt aggttttgtt ttaatagttg gaggtgcttt tggtattgta aataggactg
    14041 gagctataga agcaggaata gcttcggtta tctctaagtt taagggaaaa gaacttctta
    14101 taattccaat atcaatgatt ttatttggac ttggaggtac aacatttggg atggcagaag
    14161 agacattacc attttatatg atatttgttc cactcatgac atccttggga tatgattctc
    14221 taacagcaat agcaattgta tttataggag cagcagcagg agcagccgct tcaacaataa
    14281 atccattttc agttggtatt gcacaggcac tagctcaact tgctcctggt tcaggagtaa
    14341 cttacagagt agttattttt atagcatata tactaataag tatagcattt gttatgtact
    14401 atgctaagaa ggtaaaggaa aatcctgaaa gttcaatagt atatgaaata gataaaaata
    14461 ataaatcaat acttaatcaa acttcaacag aaataaaaga atttactttt aaagaagtat
    14521 ctgttcttgc tatatttgga atagggatgg caataatgat ttggggtgtt ttaagtcaag
    14581 gatggtacat tccagaaata tcaatgtgtt ttgctgcaat aggtgtgctt tctggattgg
    14641 ttggtagatt atctcaaatt gaaatctcag aaagttttat agatggagca aaagatttag
    14701 catctgcagc tcttgctata gctttggctc gtggaatcgt aataattgct caaaatggat
    14761 ttataataga tactatatta aattccgcag cagcctttct aggtgggctt ccaaaggtta
    14821 tatttgtata tttatcattt ttcttggaag ctgcattggc atttttaatt ccatcatctt
    14881 caggtcttgc ttcacttaca gtacctgtac ttgctcctct tggtgatttg gttaatatat
    14941 cggctcaagt gatagtaacc gcttatcaat ttggtgtggg tcttacaaac tttataactc
    15001 caacttcagg agtactgatg ggagcacttg cagtagccca tattccattt tcaaaatggg
    15061 taagatttat aatgcctatc ttgttgattt tgacagctgt atctctagtc tttttaacaa
    15121 taggtattta tatagggttt taattaagtg tagttttatt taagatattt agtaatggta
    15181 taaaatgata atatgttttc aataaattgc cactaattta attagtggta atttgttttt
    15241 tgtatttaaa aataaatgta tttacttgta ttttgaatta atatctactt agacattgtt
    15301 ttaattgtta tatttttatt taattaagca ttgatttatc
//
LOCUS       DAYASV010000091        15035 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYASV010000091 DAYASV010000000
VERSION     DAYASV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.092"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(142..285)
                     /gene="scfA"
                     /locus_tag="ACGI68_002663"
     CDS             complement(142..285)
                     /gene="scfA"
                     /locus_tag="ACGI68_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HFH0139091.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(351..713)
                     /locus_tag="ACGI68_002664"
     CDS             complement(351..713)
                     /locus_tag="ACGI68_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HFH0139092.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(771..1064)
                     /gene="yajC"
                     /locus_tag="ACGI68_002665"
     CDS             complement(771..1064)
                     /gene="yajC"
                     /locus_tag="ACGI68_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFH0139093.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1198..2319)
                     /gene="tgt"
                     /locus_tag="ACGI68_002666"
     CDS             complement(1198..2319)
                     /gene="tgt"
                     /locus_tag="ACGI68_002666"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFH0139094.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2398..2883)
                     /locus_tag="ACGI68_002667"
     CDS             complement(2398..2883)
                     /locus_tag="ACGI68_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HFH0139095.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            complement(2895..3920)
                     /gene="queA"
                     /locus_tag="ACGI68_002668"
     CDS             complement(2895..3920)
                     /gene="queA"
                     /locus_tag="ACGI68_002668"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFH0139096.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4238..5257)
                     /gene="ruvB"
                     /locus_tag="ACGI68_002669"
     CDS             complement(4238..5257)
                     /gene="ruvB"
                     /locus_tag="ACGI68_002669"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFH0139097.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5364..5966)
                     /gene="ruvA"
                     /locus_tag="ACGI68_002670"
     CDS             complement(5364..5966)
                     /gene="ruvA"
                     /locus_tag="ACGI68_002670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFH0139098.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5959..6468)
                     /gene="ruvC"
                     /locus_tag="ACGI68_002671"
     CDS             complement(5959..6468)
                     /gene="ruvC"
                     /locus_tag="ACGI68_002671"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFH0139099.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKAIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6780..7409)
                     /locus_tag="ACGI68_002672"
     CDS             complement(6780..7409)
                     /locus_tag="ACGI68_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139100.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            7612..7935
                     /locus_tag="ACGI68_002673"
     CDS             7612..7935
                     /locus_tag="ACGI68_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HFH0139101.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNKNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            8203..9057
                     /locus_tag="ACGI68_002674"
     CDS             8203..9057
                     /locus_tag="ACGI68_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFH0139102.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(9243..10679)
                     /locus_tag="ACGI68_002675"
     CDS             complement(9243..10679)
                     /locus_tag="ACGI68_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFH0139103.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSGKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNGGKEFMFRSAAAI
                     TKVLGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISSGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(10750..12132)
                     /locus_tag="ACGI68_002676"
     CDS             complement(10750..12132)
                     /locus_tag="ACGI68_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate:H+ symporter"
                     /protein_id="HFH0139104.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            complement(12268..13176)
                     /gene="garR"
                     /locus_tag="ACGI68_002677"
                     /pseudo
     CDS             complement(12268..13176)
                     /gene="garR"
                     /locus_tag="ACGI68_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
     gene            13569..14240
                     /locus_tag="ACGI68_002678"
     CDS             13569..14240
                     /locus_tag="ACGI68_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0139105.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            14343..14891
                     /locus_tag="ACGI68_002679"
     CDS             14343..14891
                     /locus_tag="ACGI68_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139106.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
BASE COUNT         4935 a         2633 c         1740 g         5727 t
ORIGIN      
        1 aaacgataag aaatcaaatt taattttaca taaatagaat atatattttt atataataat
       61 tgaaaataaa agaggatacc tcataatgat aaaagaatca ctttgagata ccctcttaca
      121 aacaactaaa tcaataattt tctatctttc acactcttga tttgcaactg tacaagaagt
      181 tttacaagct gattgacaag aagtttgaca ctctccacat ccaccttttg cagcactttg
      241 ctttaaagta gcttgagata aagtttttac atgtttattt tccattttca acgcctccat
      301 ttctaattgt ttcctattga ttatatcata aaactacttt aatataaact ctatttaaag
      361 ttgacaccca atacgcctcc aatgcctcct gctgctgata ttaacaataa cttataaatc
      421 atccctactt caaatacaaa cttttcttgt gctaaaaaca cagtcaaaaa aatacatact
      481 atgtacaaaa gtcctactaa aagcccatac ataagtcctt tttctttcat gtgttttgaa
      541 gtgtaaaacc ctcctatagc tgcagaaata gtagttataa atgatgagac catagttata
      601 ttatctccac caatatctgt aaatgtcaaa aataagttat acactaataa aacagccaaa
      661 gttattatat atgcatatcc tagaccttta aaaatataat tagattttcc cataaaaaaa
      721 cctcctctca aattaatata tattcgagaa aaggctttta aattactaat ttagtcttct
      781 tctatttctt cttttttaga tttaacagtt cctatagccc atttttcaaa tggaacttta
      841 gttctatttg gtcctaattc taatattaca acatcatctt caactttagc tacattagct
      901 actattccac ctattgtaat gaccttgtct cctacactta ggctacttct catttctttt
      961 aattgtttgt cttttttctt ttgaggtcta atcattagga aataaaagat tgcgattact
     1021 actacccata gacctatact cataattatt tgttgctgtg gcattaattt ttcactcctt
     1081 tgtaaattat actcttttgt aaatcataat aaatgtttat ataacaattt actttctcta
     1141 tctttgagta ctattgaata ttatatatta aaatgattat aattaatagt gcaaaaccta
     1201 tatttcatat ccatattttg caaaaaattc atttctaaaa tcaagtaatc tatcttccat
     1261 tatcgcttgt cttatttgtt tcattaaatt taataaaaag tgaagattat gagtagttat
     1321 taatcttgca cccagtattt catttgcttt tataaggtgt cttatataag ctcttgaata
     1381 gtttttacaa gcataacaat cacattcagg gtcaagtgga gtaaagtctt cagcataagt
     1441 tgcatttcta actactactt taccttggct agtcattgca gttccatttc tagcgatacg
     1501 agtaggtagg acacaatcaa acatgtctac tcctcttata acaccttcta ctaagtcatc
     1561 tggactacca actcccatta ggtatcttgg tttatccttt ggcataagtg gagttgtatg
     1621 ctctaacacg tcatacataa gtggttttgg ttctccaaca ctaagtccac caatagcata
     1681 tcctggtaaa tcaatattag ttatttcttt cgctgattgt tcccttaagt ctttatacat
     1741 cccaccttgt attataccaa ataaagcttg cttgtcggta ttattatggg catctttaca
     1801 tctttttaac catcttgttg ttctttctaa agaatttttt acatattctc tatctgatgg
     1861 gtatggtgca cactcatcaa atgccatcat tatgtctgaa cccaatgcat tttgtatttc
     1921 catagctttt tctggagtta gaaaatgttt tgaaccatct aagtgtgacc taaattctac
     1981 accttcttct tttatctttc ttaaaggccc tagactaaat acctggaacc ctccactatc
     2041 tgtaagtatt ggtttatccc aattcataaa cttatgaaga ccaccagctc tttttataag
     2101 ttcatgacct ggtctcatat ataaatggta tgtgttacta agtattattt gtgaaccaat
     2161 ctcttttaat tcttctggtg tcatagattt tacagttgct tgagttccaa ctggcatgaa
     2221 tattggtgtt tcaataatcc catgtggagt gtgtaatctt cctaaccttg caccactctg
     2281 cttacaagtc tttatcaatt cgtatcttac tgcgtacata aagttctcct taatcatatt
     2341 tatttctcat tatttcttaa gatatatttg taataagcta ttcatataat ttattgtcta
     2401 aatagattta tcttcgtctt ctttgttctg ttgttcctta attgcttctt catcatgaat
     2461 actcattata ttaagagcac tcttgtcttt tgttactaaa aactttttta tagcaaatag
     2521 tcctgctata gctataactc ctattaaaac atctatcata ccttcatttt taggcagtag
     2581 caacattttt ctagcaattg cgtaaagtaa tgcctcaata aatgctcctg gtatatgaag
     2641 agatagcatt actacaagtt caacacctat tactaaaagt aatatttgtg ctaaaaaatc
     2701 attaagctga ctgtattgta ctggattttg aaaatcaact atatatgctc cccacataag
     2761 tcttagaaca tctatagttc ctaaaaatac tgctattaga actactactg ctaaaacacg
     2821 ttcaaatata aaagcaatct ttaatatagg gttattcttt ttttgttttt gtaaactatg
     2881 catctattac ctccctactt tataatcata gcatctccaa aactgaagaa tctatatctt
     2941 tcttttactg cctcattata tgcatttaaa acattatcct ttccacaaat agcacttact
     3001 aacattatta gtgtagactc tggtaagtga aaattagtaa taagtttatc taaaacttta
     3061 aatttataac ctggatatat aaatatttct gtccatccac tagtttcttt catctttcca
     3121 tcctcattac aagctgattc tatagttcta caactagtag taccaacaca tattacattt
     3181 tttccatttt cctttgctct atttattttg tcagctgctt cttggctaac catataatat
     3241 tctgaatgca tcttgtgatt taaaacatcc tctactttta ctggtctaaa agttcctaag
     3301 ccaacatgta gagttacaaa ggctatatct acaccttttt cttttatttt atttaacaat
     3361 tcttctgtaa aatgtaatcc tgctgtaggt gctgctgctg aaccattatg ctttgaataa
     3421 acagtctggt atctttcttt ttcatctagt ctagcagtga tatatggagg tagtggcata
     3481 tttccaagtt catccaatat ttcttcaaaa attccatcat aatggaattt tataattctt
     3541 gaaccttcat ctgataaatc tacaacttct ccaattagct ttccatttcc aaaagaaaat
     3601 ttagtcccta cttttgctct ttttcctggt tttactaaag cctgccaagt atcttcctct
     3661 gttcttttta ataataaaaa ctctatttta cctccagttt caagcttctc tcctattaat
     3721 ctagctggta taactctagt attatttaat actaagcaat ctcctggatt taaatattct
     3781 attatatctc taaatacttt atgttcaata ttacctgtct ctttatctaa taccatcaat
     3841 cttgagctag ctctatcttc tataggaact tgagctataa gttcctgtgg taagtcaaat
     3901 ttaaaatcac ttgttttcaa tgttaaactt cctctcttaa aatttctcca gctacaataa
     3961 tatcaaaaaa acaacttatt ttccacaata ttaccataat ctatcaagaa attgctattt
     4021 ttaacagaca ccatatccac aatatataga tatttttcca cgatatacat gccttatcca
     4081 caatatatag atacaataca cacaattttt atcgtgttta tccacagtat ctacagatat
     4141 aattaccatt ttgtggatat gtttgaattt ttagccattt tagaaatatt ttaatttaat
     4201 gactctttta ttttataaat actcgtataa tctattttta tttttcattg ggatatggaa
     4261 tttttaagtg ttcatatgct aatggcatag ctactcttcc tctaggacct ctatttataa
     4321 atcctaattg aagcaaatat ggctcatata catcttctat tgtatttcta tcttctccga
     4381 tagaagctgc caatgtatct aaacccactg gtcctcctct aaatttttct attatagtca
     4441 taagaagctt ctcatctaca aaatcgagac ccaaactatc gactcctaaa agttccagag
     4501 catcttttgc cactttatca gttatttttc catcagctct gacttgtgca aaatctctaa
     4561 ctctttttag taatctattt gctatccttg gagtccctct tgaacgtttt gcaagttcta
     4621 atgctccatt agactgtatc tctgcatcta agatgcttga agacctaagt actatttttg
     4681 aaagttcatc aactgtataa tagtccaatt tacaaataac accaaatcta tctctaagtg
     4741 gatttgtaag cataccagct cttgtagtag ctccaataag tgtaaattta ggaaggtcaa
     4801 gtcttatgct tctagcagat ggccctttcc ctattattat atctaggcaa aaatcctcca
     4861 ttgctggata taaaacttct tcaacacttc tattgattct atgaatctca tctataaata
     4921 aaacatcatt ttcatttaaa ttcgtaagta tggctgccaa atctccagct ctttctattg
     4981 caggtcctga cgtaattctt aaatttaccc ccatctcatt ggctattata cttgctagtg
     5041 tagtttttcc aagacctggt ggtccatata aaagcacatg gtctaactgc tcatttctcg
     5101 atttagctgc ttctataaat atactaagtt gttctttgga tttttcttga cccaaataat
     5161 cttctaaagt ttttggtctt aaactgtttt ctatatctat atcttccatc ttcattgtag
     5221 aagttattat cctattttca tcttcaaaac cttgcattta tacccctcct ctcgtacttt
     5281 tgaaaatcta ctttatatat tttcaaattt acataaactc cttaaatatt gttttaaaat
     5341 ccaaatatta taaaatccaa atactatttc aaggatttac tcattatata tgtaagtgct
     5401 ttctttataa tagcttctgt attcatacca tcttttttac atttttgaac tgcctctttt
     5461 gattccgaca aagtatagcc taaagctaca agtgcatcta cagactcatc ttgtgatatc
     5521 aaggtaggtt tttgtgataa taacgttggt tcaaactcta tgttattttt gtctacctta
     5581 tccttaagct ctaagacgat tctttctgct gtctttttac caacaccagg agctttggat
     5641 aattttccaa tgtcttcact taaaatatat gctcctaact gtgctggtga tgcaaatgat
     5701 agtattgaaa gcgccacctt aggtcctatt ttagatacag atgttagtaa ttcaaacatc
     5761 ttaagttctt ccctacttac aaaaccacaa agactcatat catcttctct tacaattaat
     5821 ttagtatata tttttgtggc ttctcccact tgtaaattca ttattgtatt ggatgataca
     5881 tttattttat atcctatccc attattttca acaactatac tgtctatata aatttcttct
     5941 actgtacctt ttatataact atacatactt accacctata ttctttagag ttttttcaag
     6001 tttgtttgaa tgagcatggc aaatagccac agctaaagca tctgcaacat catctggttt
     6061 tggaactttt tttagactta aaaatgaagt taccatctgt tgaacttgtg ccttatctgc
     6121 tctcccatat ccaactacac cttgttttac ttgaagtgga gtgtattcat atacaggctt
     6181 cccattatga gcacatgcaa gcatagtaac acctctagct tgtgcaactg ttatagctgt
     6241 ttttacattc ttgttaaaga ataattcttc cattccaact tcatctatat tgtaattctt
     6301 tactattgta tcaattcctt tatacacaag ttccaatctt ctcgatatat tcatatgggc
     6361 aggtgttgta actgctccat aatcgattgc tttaaacttg ctatttttgt attcaattat
     6421 accatatcca actatggcta tacctgggtc aatccccaat attatcattt tttaaaattc
     6481 cccctaaaaa taaaagaaat accaaaatgt atttctcata aaaacttatg ttctaaatca
     6541 ttatatcata aataatatta acaaatcata aaatgctcta tgtaaaatat aaaatcaaat
     6601 aaaaaagtta tatcaaagca agattttacc tcaatataac ttttatattt attacaaata
     6661 aattattgta tttttatagg aattaaatgt gactacaatt ttaatactaa tctatttaat
     6721 attaaattat ttattattaa tttacttagt atttaactta aaataatatt aatttactat
     6781 taacttgcaa agtcttctaa tcttgaatag gcctcttctt cagtatccat aactacacct
     6841 tttttgattt catcttgaac tgttttagga agtgaatcta ctcttatttt tgttttttct
     6901 actaatgttt tacttccgct atcatcatat ttatatagtg ctatatttcc attatcattt
     6961 tcaatagtat atttaccttt tatagtaaca tccattgctt gtacatcttt attatcacta
     7021 tatttagatt ctaccaatat gatttcattt ttatccatac tctttatttt tttagttgga
     7081 tattcttttt taaaatatga tactatttct gacttcgtct tatctaataa tgactctgga
     7141 actattcctg ccattgtaag tgttctctta tagttagttt cattttcgcc ttcactttga
     7201 agccaaactt cacaattttt atttaatcca aaagcttctt ttgacttttc attcatattc
     7261 gcactagtac ttacaacttc accagtttga actccattgt gttttaatct tgtccctata
     7321 taaactccgc tacttaatac aaatactcca accagtatta aggcagatat tttccaagga
     7381 aataattctt ttttcttctt ttcaaacatt gattacgcct cctttttcta ttattttctc
     7441 caagctgtaa gtttttatac aatttaggta aattttagaa gaaaatatat ttatatttca
     7501 aattactcta agaatagata ttttctaaaa ggtagttttc tcttaatggt tatattcttg
     7561 taattatatg gacataattt ataataaata atcgtttagg aggtaataac tttggaaaga
     7621 ataaactgta atgtaattaa ctgttcacat aacaacagcc atatatgtta tgcagataaa
     7681 atttttataa atggtgaaaa agctcacaat gacagacata cttgttgtag ttcattttta
     7741 gatgaatcta tttatagtaa tttatctagc aacgttaata ataaaaattg tgaggaatta
     7801 gtttgtactg taaaaaattg taaatacaac aaagatggta gcttatgtac acttgataat
     7861 atattggttg ctcctgaaac tgatagagta aacctttata ttgaaacatg ttgctctagt
     7921 ttcgaatgta aatagtcata gtataaatat aagggtgtca aatgtaatta aactaacata
     7981 gtgtagagta tctacaacta ttagtttgat tatacttgac acccttattt attttaaata
     8041 aaatttcatt attataacca tgtttataat gttctaactg taattaaatt tgataaattt
     8101 ctataaattg gtataatcaa tatgtaaatt gaatttagta aattaatcat aaaaataaaa
     8161 tatcgagtat atagtgttat actaactaga aatgaggtgc ttatgagtac attttatatt
     8221 caatttcttt attttgtaat ttatagcttt ataggatggt gttgtgaaac tacatattgt
     8281 tcagttttac aaaaacaatt tgtaaataga ggttttttaa atggtccatt ttgccctata
     8341 tatggatttg gagcattagc aatagttgct accttgactc cttttgtaca caatattcct
     8401 ttgttatttt tgtttagtat aattataact agcataatgg agtactgtac gagctttatt
     8461 ttagaaaaaa tatttaatat gacttggtgg gattattcta aacataaatt taatattcat
     8521 ggaagagttt gtctggaaaa ctcacttatg tttggtgtat tatcattgat tgtaatgctt
     8581 atagtacatc ctattgtggt tgactttatt aactctatct ctaaaaatgt gctatttata
     8641 tttgctatat ctatagaaat ttattttgtt ttagatttag ttatcacagt ccatacaatt
     8701 ttacaattaa atggtaagtt aaaacaaatc aatcttataa ttaaggaatt aaaagataag
     8761 aaagaatact acaaacttat tacacaagaa actatagaaa acaaacttga atctttagtt
     8821 gaaaatagac tggatacttt ggaagatagg tttgaaatac ttaaagaaaa agaaaattat
     8881 cgctatgcta aaaatcgtat tgatgagcta aaaaataaac ttaatacttt gctgtctaac
     8941 cacaaattac ttcatagacg tataatcaaa gcattcccca acataagctc taataagcat
     9001 acagatattt taaataaaat caaagaaaat attaaatcaa ttaaaaaaga taaataataa
     9061 aataaacaca taagttaaat aataaaaata ctaatacata agattaaggt ttagaatatt
     9121 ttttgtaaaa tactctaaac cctaattaat atactattat ttttaatctt aatttataat
     9181 tttagtttat tttaatttta actcagtaaa tttaagcgtt taaacttcaa tacactaata
     9241 tactacccca tcaatatctc aactgattct ctaagtgtgc taatctctcc cacatttcca
     9301 gggaaaatta tatatggcat attaggaaat ttgctttctt tgcctgtcat ccatactggt
     9361 ataccaggtt ttatctgtcc cattactgta gcttttttta ctcttagtgc tttagtacca
     9421 acatcactag aagtaattcc acccttagct ataataaaat ttggtcttac attaagcata
     9481 ccaattatac tggtaactgc atctgatatt tctacagaaa tcataagctg tttatcttta
     9541 tcattcgtat ccaagtcaaa acgctctcgt ctagtataaa cagcaacagt tttaccactg
     9601 cttatttttt cctctacaac ttttataact ctttttacct cattttttag accattttct
     9661 tgaagaacta aatgttgatt aaattctata aattctatcg gatatttaca atttttaagt
     9721 tcttctaact gttgggtagt cttattcaca tgtgaaccaa caagtataat tcctccattg
     9781 gtattacctt ttgatactaa ttcatcctta gtaagtagtt ccttgtcact cactccgcca
     9841 agtacttttg ttatggcagc agcgcttcta aacataaatt ccttaccacc atttactgct
     9901 cgtataaacg caattgtaaa tacctttaca tctacataat cggttgcatt tacaataatt
     9961 ttattgaatc cttcagcttt tttaagtttt tctgttatag cttcaatatt tagctctcta
    10021 agttcttttg acgaaatata tatcatatca ctagatttgt actttccatt tgtcttttct
    10081 tcacaccact cacctatatc agatgattta tatccaaaag atttatcttt tgcaaattca
    10141 gtcattcctg ctggtacaag tatttctcct tctttaacat aatgtacatt atctatagta
    10201 aacctaccac cttctttaaa aaatggcatt attatttcac cctgaaataa cttccccgat
    10261 aatctctcta cttcgctttt taataaatta gtttcaagag gataatgtcc tctcatagta
    10321 gaatcactac gacttatgat tataaaatcc ttattaactt tctttgatac atccacaata
    10381 ttctttgaaa tttctttatg tgcttttgta gtttgagcaa cagtaaatcc ccttgaattg
    10441 gtaagtataa aaaacatact atttttctca ttaaagcctt gttccatact atccttatcc
    10501 caatcagtat aaacagaaat atcatgaact gtttgtacac ctgttgggtc atcatctaat
    10561 acaacaattt ttctatctaa atgcttaagc tcatctgcaa gaagtttatt taatctatcc
    10621 tcatctactt gtttaaaagt attaagtata tctatgctaa ttggttttac ttctctcatt
    10681 taaaacgccc ctttatgtaa tttgtatagt ctaaatataa tttatatgat ataaacaata
    10741 gtttgttatt tatagacctg gaagtggtat aattccattt ataacactaa gcaataaaac
    10801 cattataaat gctacaactc cagcaatggt tcctccaatt gtccaagagt ttaaagtatc
    10861 tttaactgat aatccactaa atcttgaaag tacccagaat ccagaatcat ttggaaggga
    10921 gaaaccaact cctccagcac atatagcaag ccctactaaa actggtgaca ttccagttgc
    10981 caaaattgta ggtcctaaaa ttgatgatgt tgttactaag gctacagtag cagaaccttg
    11041 agaaattcta agcaaggcac ttaataagaa tccaagtact acaactgata tactaagacc
    11101 agacatagta tcaactaaga agttacctat accacttgca tttataacac tgccaaacgc
    11161 accacctgac cctgttatca ataatattag ccctgctgaa tcagcagctt caataactac
    11221 atctcccata ggtttattta tgtatttctt taaaaatcct attgccacca atattccaag
    11281 aagtattgct atgtttttat ctcctataaa tgcaagtact ccactcattg tagaaccttt
    11341 ttctaagaac atactaaata catttgatat aagtataaga agtattggga acaataaaga
    11401 aaacatagaa attccaaaac ctggtacttt agaagaatca tcatcttcaa tttttatgct
    11461 atcttttccc tgctctaaat ttttttctct atcattctca tcaatagcat aagctggata
    11521 tttttcaaaa cgttttccat aaatccatcc acctataaga gctgctggaa aagcaacaat
    11581 tatagaataa agtataaaac tacctacatt tgcatttaca tttgctgcaa ctgccaaagg
    11641 tcctggtgta ggtattacaa gtgcatgacc tactatagtc ccaactccta aggcacatac
    11701 aaaaaccatt agagatttct tagttactct cgaaacatac ttaattatag gcatcattat
    11761 aacaaaagct gcatccataa aaactggaat tgaaattaaa aaacctgctg tagttactgc
    11821 taaagcactg tttttttctc cagtcttagc taacaacccc tttgcaatag attctgttgc
    11881 tcttgattct gaaagaatat ttccaaatat tattccaagt ccaattacaa tacctatact
    11941 acctaatgta tttccaaatc cagtcgatat tgtggttgat atttcattta taggcattct
    12001 aaccataaag cctgtaatta tagatgttat cagtaatgcc aaaaacggat ttactttaaa
    12061 tttaataatc gatacaagta aaattcctat agatataaca aatgctatca aaagtccata
    12121 tcctgtaatc ataatcttta cccctttatc attctacttt attatttatc aattttattt
    12181 ttcttttact atgataactt aacctcgctg tatttatata atctaatcct aataatatgt
    12241 atttaattat attgatatat ttaaatatta attatcgtgt ttttttattt caacacctgc
    12301 caacttttca aaatactgtg ctattccact atggtcatct tccaaatgtc catgtacttt
    12361 taatgtctgc ataatttcta ataattgact tgtcataggt agtggtacat ctaaattgtg
    12421 tgctgtttgc attacattct taatatcttt caaattaata gatatttttc cacctggttt
    12481 aaaatcacga ttcataatca ttggtatctt cacatccaat attgtactac cagctaggcc
    12541 tcctcttatt gccttatata ctttttctgg gtctgcacct gcttttgctg caagtacaaa
    12601 tgcctcagaa actgctgcta tcgtcaaatt aactattact tggtttgtaa gttttgtaac
    12661 acttccacta ccattatctc caattaaaat agatgagctt cccattatat taaaaaatgg
    12721 ctctactttc ttataaattt cttccttacc accagccata aaagcaagtg taccatcaat
    12781 cgcttttggc tcccctccac ttactggtga gtctataaat tctaagccca tatcttttaa
    12841 tttatttgca cacaatattg attctgtagg agtaactgaa ctcatatcga ctactatact
    12901 tccttctttt aaagtttttg ccaatccatc catcccaaac aatatatcct gcacaatagt
    12961 tccatttgga aggattgtaa atacaatatc acattcttga cccatttcta aaacacttgc
    13021 gtattttgct ccacatgata taagttcatc cacagcagat ttattaatgt catacaccaa
    13081 aagattacat ccatctttta ataaattttt tgccattggc tttcccatta tccctaatcc
    13141 aataaatccc aatttcattt tataaccctc catatcttca catttttgaa aacgtttttt
    13201 atgttttaga gtatactaca taccagtata ccggtcaata taaaatataa aagtatttat
    13261 tttaacaata aatagtaatt tttaaagtca tttattatat ttagtcatcc tatataagtt
    13321 ttaatacata ctattgtttt caaatataat atactacttt tacagttttg tattttaact
    13381 aatttcaatt agttttaatt aatctgcctt taatgttaat catgcttcac acttcaataa
    13441 ttttttatat tttttaatct tatctgcaat ttaaattgca aaagattgac cttattttca
    13501 ctatgttgta tgcttaaaat tatataagtt tttgtatttt tatattgttt aggggggtat
    13561 tattaagtat ggctacattt aaaagattac aagaacaagc atatgactac ttaaaagaat
    13621 tgatactatc tggagaaatg atagaaaatg aaatatattc tgaaacaaaa cttgctagtg
    13681 aaataggtat ctctagaaca cctataagag atgctcttca aagacttagt caagatggat
    13741 ttatagatat aatcccaagc aaaggattta gaatccatca aattacagca aatgaaattg
    13801 tagagatatt ccaaatacgc tctgcaatag aaggattttg tactttttta attacaagtc
    13861 aatacaaaga agctagagca gtagaaacta taagtaaatt aaaacatctt ttagataaac
    13921 aaaaagatat tttattaggt gataaaaatt taaactcatt tgcagaatac gatacattat
    13981 ttcacacaac tatagtttca tatgctcaaa atactgaatt tgacaaaatg tttaacaatt
    14041 atatgtacag aataaaaaaa ttagctctcg attcattgtc acatgaagga aggcttgaaa
    14101 caacattaaa agagcacaca gatattttta ataatattgc aaatggatgt attgaagata
    14161 tttacagaac tactctcatt catatggaaa ctcctaaata cattaattta gaagattttt
    14221 gtaacaataa gttattttag tattatactt tcatttatta aaaatatatc tatctcaaat
    14281 taatttccta atttataaaa aatattctat aattatattg taactaatac ttattctaag
    14341 gagtgatttt tttgaacgaa gatgttgtaa ctaatgaaac tattgacaaa gattatgcta
    14401 ttgaagaagt tcgtatggca tgcaaacatt ttggtgattt atatttttat ttttccaaag
    14461 tgttatttga agagtttggt gaagataaaa ctagcgaaat attaagaaaa gttttatttg
    14521 aaaggtctga agaaagagct attgctatga gagagagagc tcatgaaaat ggagatgaat
    14581 taatcgcaga caatattatt agcacaactg atgttccatt tctaggatgg gttcctgaat
    14641 ttgaagagtt acattgcccc tatggagctt cttggctttt aagatttgag gaaaatcctt
    14701 ggtttaaaaa atttgcttct ctttactgtg atgttacaga tactactgta gctgaagtat
    14761 ttacaggaga tacgtcccat aaaataacaa aaaacattct atggggagat aaaagttgtg
    14821 aaagaatata tttccatgat gacaaggtat ctgaaggtaa atatacttat ggaaataaaa
    14881 actgtaaata aatgatttaa attttctata ttcatatact atattttgta ctttttcata
    14941 taaatattat aactagtctt ttattttact tttgtttaac ctaaagataa tttactttta
    15001 atctatatga attatcatat aaataatcag aaagt
//
LOCUS       DAYASV010000092        14552 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYASV010000092 DAYASV010000000
VERSION     DAYASV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.093"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..602)
                     /locus_tag="ACGI68_002680"
     CDS             complement(<1..602)
                     /locus_tag="ACGI68_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFH0139107.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINS"
     gene            complement(873..1649)
                     /locus_tag="ACGI68_002681"
     CDS             complement(873..1649)
                     /locus_tag="ACGI68_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFH0139108.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLIDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     NTVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            complement(1706..2677)
                     /gene="asrC"
                     /locus_tag="ACGI68_002682"
     CDS             complement(1706..2677)
                     /gene="asrC"
                     /locus_tag="ACGI68_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HFH0139109.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            complement(2690..3481)
                     /gene="asrB"
                     /locus_tag="ACGI68_002683"
     CDS             complement(2690..3481)
                     /gene="asrB"
                     /locus_tag="ACGI68_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HFH0139110.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            complement(3482..4489)
                     /gene="asrA"
                     /locus_tag="ACGI68_002684"
     CDS             complement(3482..4489)
                     /gene="asrA"
                     /locus_tag="ACGI68_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016002 - sulfite reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HFH0139111.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            complement(4880..5569)
                     /locus_tag="ACGI68_002685"
     CDS             complement(4880..5569)
                     /locus_tag="ACGI68_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0139112.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(5632..6480)
                     /locus_tag="ACGI68_002686"
     CDS             complement(5632..6480)
                     /locus_tag="ACGI68_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0139113.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            complement(6499..7386)
                     /locus_tag="ACGI68_002687"
     CDS             complement(6499..7386)
                     /locus_tag="ACGI68_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0139114.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGKDATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            complement(7444..8247)
                     /locus_tag="ACGI68_002688"
     CDS             complement(7444..8247)
                     /locus_tag="ACGI68_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFH0139115.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            complement(8407..8859)
                     /locus_tag="ACGI68_002689"
     CDS             complement(8407..8859)
                     /locus_tag="ACGI68_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFH0139116.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            complement(8989..10491)
                     /locus_tag="ACGI68_002690"
     CDS             complement(8989..10491)
                     /locus_tag="ACGI68_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFH0139117.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            complement(10524..11405)
                     /locus_tag="ACGI68_002691"
     CDS             complement(10524..11405)
                     /locus_tag="ACGI68_002691"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFH0139118.1"
                     /translation="MRTTDMKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGL
                     YVGGSTGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGY
                     DAISAVTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENE
                     KIIGVKFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRA
                     RQIFELTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKE
                     ATDEMKSRAKEIYRKYF"
     gene            complement(11475..12146)
                     /locus_tag="ACGI68_002692"
     CDS             complement(11475..12146)
                     /locus_tag="ACGI68_002692"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFH0139119.1"
                     /translation="MLDKVKGRLIVSCQALEDEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            complement(12419..12910)
                     /locus_tag="ACGI68_002693"
     CDS             complement(12419..12910)
                     /locus_tag="ACGI68_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0139120.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            complement(12932..14296)
                     /locus_tag="ACGI68_002694"
     CDS             complement(12932..14296)
                     /locus_tag="ACGI68_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0139121.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
BASE COUNT         4760 a         2605 c         1355 g         5832 t
ORIGIN      
        1 gaatttattc tataattaca gctatctatt tttctcatac tagatttaat tatagaacta
       61 tcatcattaa tagtattttt taaaagacta attttttcta ataattcatc acatatagat
      121 atcagttctt tatctccgac atttttcatc ctatttctat tcataacaaa ttgagcaatc
      181 attattataa ctgctccaac agctaaagcc gacaatctca taggaaattc actttttcct
      241 acaggtatac ttaccataaa tatatacatt agaccaaagg gtacatatac tgctcttttt
      301 aagtcataac taaatacata ccctattata aataatgcta taaaatttaa taaaactcct
      361 aaatacatat tttgtactgc aaaaaatgat agtattccta agattatatt tattgctaaa
      421 tacttcagta aatttttaat tggagaaata gttaaatctc tttccaagag cgttagcata
      481 gctgttacta tacctacacc tataagtgta tttttacttc caaataaata tataaatata
      541 gaaataaatg ctattataaa aataaacaac tttgtttttg atattactag tttctttgtc
      601 atcatatccc ccctaaaaat ttcaacaaga ccaattttat catatttgtt atcttattca
      661 aaatgcgctt ttttacggta ggactttaac tttttttata tttagaacta tataattcat
      721 atataaacgt attttttata tatataaccg atatattttc gtatgctccc atattttttt
      781 tgtttcaaat attttttcaa aaatataaaa ataactctct caacaatact aaaaatgcat
      841 ttatcaagag agttataatt tatacaataa atttaattaa gcttttcttt acctaatata
      901 taagttccaa gacccataaa taaagctcct ccaacaatat ttccaagtgt tacagctact
      961 aaattataaa tagctcctcc aatagtaact gtatttattg ttggtgaaaa taatgaaaca
     1021 ctataaattg tcatatttgc tacactatgc tcaaagcctg atgttataaa agcaaataaa
     1081 cataagaata tcataattat ttttgcagta tcactattag ttctaaacga acacaacact
     1141 gatacacata ctagaatatt acataaaatc cctctaaaga aaagagctgt aaatggcatg
     1201 gatgctttac tagctgctgt atttgcaaaa aattcagcta caggtccttt gtctattaga
     1261 ccagttccta caaatataat gcctaaaacc aaagctccga ttaaattacc tacccaactg
     1321 tatgcccata ttttagaagt atcttttata cttacacctt tatttaacat accagcagac
     1381 ataaccatat tgtttccagt aaataactct gttcccgtca ttataaccaa acttagtgct
     1441 attgcaaatg ataaccccat tactatctta gtcataggtg aacctgcatc agtcaaaagt
     1501 cctccaatag taaatatcaa aagtatacct atacctacat aaagtcctgc aaacgcagaa
     1561 gataccaaat attttacttt acttgtgttt aataaattta tcttatttat agctgcatta
     1621 gttaatttat ccaaagtttc tttatgcata attccctctt ccttctatta aattttaatt
     1681 tattcaaata tatgttttac tcaatttata gatatttaat tccactccag tatatgtttt
     1741 cgtacatttt agttatttct ggaagctcta caccttcaag tgcccatttt ttaaattcca
     1801 taaatcctgt tctatcaatt atatatccta tatgttcttt tcctcctggt gcatctttat
     1861 ctatatactt ttctacatat ctataagtat taagtattat ctttattatg ctgtcctcat
     1921 ctacccataa aagaaaatct tctgctagtc tagggttttt ctttcctgtt ctacccatta
     1981 tagctaatct atagtatttt ttctcatctc tagtccatgc atttgttggg caatttagca
     2041 cacactctcc acagcctata cacttatctt tatctcttac caccttatta ttttctgttt
     2101 ttaatgctcc aacagatatc ttagaacatt tttttacaca tgctccacaa tttacacatc
     2161 tatccttttc atatattggt aaagccatac caataattcc aaagtcattc attctagctt
     2221 ttatacaatc atttggacaa ccagttaaag ccactttaaa atgtaaatca tttggaaata
     2281 tagctttttc tattctttta gcaaattcag ttgtattata ttgagccttt ggacagactt
     2341 tatttcctat acaagctgct atatttcttg tacctgctgc tggataacct gcgtttttct
     2401 cactttgatt tatattcatt ttttcaataa caggttgtat aattttattt atttcttcca
     2461 tatcttccat atttattcct aatatttcaa acccctgtct tgtagttatg tgcacctgtc
     2521 cattgccgta ctcacttgca attttagaaa ctacagctaa actctcaggg tcaataagac
     2581 ctccaggtac tctaactctt aaagaagtct catattttgt ttttgtaatt ctataagcat
     2641 ttttcattac ttttttggta tttaaatctc ttatcacaaa cttcaccccc taatcaataa
     2701 gatttttagc atatgaataa tcaaatactg gtccatctaa acatatgtaa gtatcatcca
     2761 ttttacaatg tccacattta cctattccac aacacatttt tctttcataa gaaacccaaa
     2821 tgttcttttc atctaaatct ctttttaaaa attcttctac tgtaaatttc atcatcattg
     2881 gaggtccaac tacaactata gaagtattgt ttatatcttt catatccaat tctggtatgt
     2941 attttgttac taatcctaca ttaccactat atccttcctc tgctccatct acagtaacta
     3001 aaatatcaag ttttttactc catctcttta aatcatctgc aaataaaata tcttttggtg
     3061 atttaaatcc tactattaat ttaaactttt cgcatttttc tttattatta taaaaatatt
     3121 caactattcc tcttacaggg gctaatgcac ttcctcctgc cactactaca atttctcttc
     3181 cttcataaag ggatacatca aatccatttc cataaggacc tcttataaaa aaagaatctc
     3241 cttctttata attaaacaac tcgctagtta cttttcctac atttcttatt gtaaagtcaa
     3301 tataatcctc tccatatcct gatactgata ttggactttc accgtattta ggtatagata
     3361 tctcataaaa ctttccaggc aatacacctt ttgtattgca gtttactcta aaagtccatt
     3421 ctttatcagt atgtttaact atatctagta tttttgcact tactggtata tatgggttca
     3481 tttatacttc ctccttacta attactaaat catttatttt ttctatacac tttgaaaatg
     3541 atatgtattg cggacaggca tcatcacatc ttccacagcc aacacacata tgatatccga
     3601 atcttttttt aaaatcatgt attttatgca tagttttaaa tctcattctt tcgccttgtt
     3661 tttttctaaa agaatttcca cctgctatat ctgtatatcc atctacttga caagaagccc
     3721 aaacccttct tctctcacct acattttcgt tttccttata gaaaatatcc tgcattgtaa
     3781 aacatgaaca tgttggacat acaaaattac atctaccaca tcctatacat cttgtatcat
     3841 attcatccca tattgaattt cctatcattt catttgaatc aatattctct ggcacatcta
     3901 cagatattaa attatcagtt acataatcaa cattaaattc agttgcttct ccattaaata
     3961 cttcaaattc actatccttt atatctaagt acagtgtatc tccttctata tttaatccaa
     4021 ttgaataatt atcagttttg ttagaatcca tgctcacaca aaaacagttt ctaaaacttt
     4081 ccttacaccc tacaagtgca aactttactt tatctctgat atttttataa aaataatctt
     4141 tttcttcacc attatttaga tatatttcat caagtctttt tataccattt atgtcacaag
     4201 ctcttaagaa aattaaaatt ttcttgttgt ctaaatcgct ttccttaaac tctttttcag
     4261 taaaataaaa caatacttga tttatgggaa gtacaacttc ttttggtgaa aagtttgctt
     4321 tcttagcaaa ttccatttct tcaaaagcgt ttacctcctt gtatcttata acatcagtat
     4381 ctgaaaaagt acctttaaat gggattaaaa ctggtgcaaa tatcttgtag tcttttttta
     4441 gataattcag accttcattg aacttagatg aatctaattt tagtttcatt tttatcacct
     4501 ttcttatatt ttagttttac ttacttgcta tcttgtttaa atcttaacac tttattttct
     4561 atgcttccgt gataattatc acagtttcaa aaataataaa aaaaatatta atggagttta
     4621 ttaagttctg ttaatatttt tttattatct acattattcg atttcaaatc taatcactta
     4681 aatacaatca ttaagtacaa taaaactact atctactcaa ttttttgaat aaaaaagaat
     4741 cttatttgtt tttttataca attcaatagg gtaaaataaa aattaataaa ttagacaata
     4801 aaaaagaatc tataaataga ttctcttaat aatattttta tttaattaaa attctaataa
     4861 tactcttact taatcataat taattatttt taaaatacct tgataattca gtttctttaa
     4921 cataaaaacc tttattgtca attttaataa gttcttcttt ttgcaatacc tttatcattc
     4981 tggaaacagt ctccctttta cagccaagca tatcagctaa atatgtaact gtaatattta
     5041 catttagtaa agtccattct ccacagcaaa ctccaaattc tctacttaac ctatataatt
     5101 ttgctgcaag ttttttatct acttttatgg atatagaatt tttcaactgt ctatataaac
     5161 gtcttattct tctttccata taagaaataa tatcctttgt aagttgaaaa tcattttgca
     5221 taatttctaa aaattcttca atactataat aagctacaat tgaattttca aatgcttcac
     5281 agccaacagt agagttttta gaagcgtcaa gtgtaatatc atttatgact tccccactat
     5341 ttaatatgaa aattactttt ctctctccat tttcacttat cttaaaaata gatacctttc
     5401 cttctaaaag tatataaaca ctgtctaagc tatctttttc ataaaataaa atttcttttt
     5461 tttgtaatct ttttagttta actttttctt taagttgatt cttcgtttta gtacttaaat
     5521 tattaaaaat tattacttta tcaatattat aatcttttaa tttagacatg taatatctct
     5581 ccattcctaa atttttaaga gagctctata aaacattaca aatttcttat tttattcatt
     5641 tttaaacaat tttatagcat ttgtagtttt aattttcctt tcaatagctt cattagaaag
     5701 ttcttttact acctgtgtat atactaaatc aataatgaaa aattgtgcta atttagaaga
     5761 tattgaacca gtttccagta ctgtttctcc cgatacataa gataagtgta catctgaaat
     5821 atcctttaat tcagaatttt tattttcagt aattgatata attttagcat tattggctct
     5881 tgcaagcttt acagttttta taatctcttc tgtttcacca gaatgtgaaa ttgcaattat
     5941 taaatctcct tcttccatta tggaagacat cattatcatt gtatgactac tgtcaaaact
     6001 tacacaatta agacctattc tcataaattt atagtttgaa tcctgtgcta ttataccaga
     6061 atatccaata cctatgaagt aaactttctt tgcatttatt ataagccttg cgcagtcatg
     6121 tacatcctta ctattgatta tctctactgt gttctctaaa gtggttacat ttgaacttaa
     6181 aagttttttt gctgtatcca aagctggctc atcattttgt atatttttat ttataatatt
     6241 ttttttattg atagttgata tttcttgtgc taaagtaact ttaaaatcct gtaatccatt
     6301 aaaattcatt ttttttacaa acctagttat agttgcttca ccaatattgc tctctttagc
     6361 tatttgtgat attggtttat aaaatacatc atctatattt tcttttatat aagctattaa
     6421 taatttatct gattttgtga ctttaaaagt tggtgattcc aattgttcta aaatactcat
     6481 taaaaaccct tctattcttt atattttgtt taaaaaatta tataatgctc caagcattcc
     6541 tgcatcattt tgtctctttg caaattctat attagtgttt ttgtaaactc tctctataag
     6601 aacctccttt aatgctttgt ctatttttgg ttttaggaac ttttcttgtg ccataatccc
     6661 accacctaaa attaccactt ctggatttat tacataacat atatttgcga ttccaactgc
     6721 taatgatttt atcatattat cgagttcttt taaacaatct tcatcattat tctttgctaa
     6781 atcaaaaatt atttttccat ttaagtcatt ttcattcaaa ttttttattt ttgctacttt
     6841 tttaattaga ctgctagtag atgccaattc ttgaaaagaa cttccattta cattcatata
     6901 tcctacttca ccagcactat tactaaaccc atttatcagt ttattattta ttataataca
     6961 tccaccaatt ccagtaccta ttgttaaaca tatagaagat gttgcatctt tacctgctcc
     7021 aagccacatt tcaccaagtc cagcacaatt aacatcattt tcaacttcac attttatatt
     7081 aaactctttt tctacttctt tttttaattg catgccctta tagtttggaa ttaattcttc
     7141 taatgcaaat agtattttac cttcaactgg gtcaaccata cctgcagttg atatacatat
     7201 accacttatt tcattttcct ctatatattt ttttactata tcttttattt tggctaatat
     7261 tccttctcca cctttttcct ttgcttcagt atcaagactt gatttatcaa ttataaatcc
     7321 atcttctcta ataaaaccat attttattga agtaccccca acatctatac aaacgtaatt
     7381 tttcataaca tatctccata caaattattt ttataaatta acaactctat ttaaaatttt
     7441 atattacaca aatcttttac ttatcttttc caattcacag atatatttcc ttgcttctga
     7501 taagtctata gtatcactta atacactttt ttctttggtt atatgatact tataaatatt
     7561 tttacataag tcctctccta acatattaag accttcttga gtgtaatgaa catcatcaat
     7621 agttaatcct ctgtcatgaa actgcatagc aattttgctt cctacacata atatatcaca
     7681 ttcactacaa gccgactcct gtgctgctgc aattcgtctt gttctaataa aatattcatc
     7741 ttctccaagc caataaccag ttaaacttac aaacatttta tcaatcatac attcttcttt
     7801 aagcctatac gcaatttttt ttagactttc tttatagtac aatacatcac taccatattt
     7861 tgcatcactt tcaccttgaa tccaaataac gtatttattt tctatattat aatgtttact
     7921 taaggtagta cagcctgtat taaatttttc tatagtgtca tttagaaacc agttattatc
     7981 atgattccag ttttttattc ttgaaccatc ttttgcattg tgaataaaac aaactttatt
     8041 tccagtaagc ttattccatt catttgaaaa tgcaggtgct atagtaccat caccttctga
     8101 agtctctagc tcacatctca tatttataat ttcatcagta ctcaaatatt caaaacaatt
     8161 ttcatttgga atgatgctct cttttggatt tcccaatcct ctagcattgc tttgtcctat
     8221 taataagaat acatctatat tttccataag ttaactcctt tacaaaattt taagctttta
     8281 attattttca attttctcat aaaaaactaa ggctgaaaat agtaatctaa ctaaaattta
     8341 cgaagtctca ctaatcgctg aaagttaaat cactattttc agccctagtg gttatataaa
     8401 ttatcattat ctaaccaaaa ccttaaaaat agcttttttg atattttctt tttctaaaac
     8461 ttttattcct gtcatatgag catcttccgg ataacaaact aaaaaatcac cttttctaag
     8521 ttccatgtat ccattatttt taccatcaag aggcaagaaa tcgccctttt cttcatatat
     8581 tttttgttct aaattttcta taaaatttat atctattcgt tcacatccat caagcatcaa
     8641 atgaacatct atatacttct tatgagcttc ccagaatcta tcttcttttt cacaagtttt
     8701 atactctaca atattcacaa aaatatcatc accatctatt gtataagtac ctttttcata
     8761 atctattaat gaattatctt tagcatattc aaaacatcta agtaaatctt catgcaatgt
     8821 tgaatatgtc ttactatgat ttatatttcc aaatatcata agcactatct ccctatataa
     8881 ttataaagtc tatttttata cttttatata caaagtatat tatacaatct tatagagtaa
     8941 ctaaattatt tatctaatca ctctataaga caatttatca tttaaatatt atttttcata
     9001 ataaacagta gtctcagctt ttggtaaaga tacttttgct ttaactttag cttctattgc
     9061 actaactatt gtacctacca ctacagaagt cgtgattgtt attagagaat atgcccaaga
     9121 agttacttct ggcatacggt attttaaata aacaactaaa attgctgata ctatgaatcc
     9181 aacatatgct cccattttag tcgctttttt acaaaatgca ccaagtacaa atattcctgc
     9241 taaaacacct agtactagcc ccataaaact attaaaccat tcatatgctg actttatttc
     9301 tccatttgct aatactattg caacagctat agaaagtata cctacaccta atgatatata
     9361 ttgtgctatc ttagtttgct tttccatact tatatctttt gttatgatag tttgtatatc
     9421 taaagtccaa cttgtagcaa ctgaattaag ccctgtcgat aaagttgatt gagaagctgc
     9481 atatatcgct gcaagtagta ttcctgttat acccactggt aattcataag ctatatatga
     9541 tgcaaatact tggtcttgtt gaactgtttg agctaaatct gggttttgag tatagtatat
     9601 aaataaacaa gttccaatta gataaaatac tgttgcaagt cccattgata aaacaccatt
     9661 tccatatgtc attttattaa gttgctttat atcagttgtt gtagtaaatc tttgaactat
     9721 gtcttgactc gatacatatg atgaaaaagt attaagacca gctccaacaa atattataaa
     9781 tgcacttgta gatagtatat ttttatcaaa cataacttca ttacttgcta agaacttgtg
     9841 tccagttgta agagtatcca ttactgttcc aaaaccacca tttatacttg ctattaaata
     9901 gaacaatgtc cctgtaacac ctactattaa taccattcct tgtataaaat cggtccacaa
     9961 gacagccttt aatccacctg tatatgagta aattattgct attacaccca taactattat
    10021 cagtacattt acaggtattc ctgtaagacc agccagaact attgatggaa gatacattat
    10081 gatagacata cgtcctattt gatatattat aaacatcaaa gctccaagta ctctaagacc
    10141 tttgctttcg tatctcattt ctaagtattc atatgctgta tctatttcta acctactata
    10201 tagaggtaag aaaaatttta ttgtcaaagg tattgctata aacataccta attgtgcaaa
    10261 ccacaaaatc caagtaccac cataagaatt tcctgctaat gataaaaatg atattggact
    10321 taataatgtg gcaaatattg atactgatgt aacccaccac ggaatagttc catcaccttt
    10381 gaagaactct tttcccttca tttccttttt tgaaaataac aatcctgcaa ataaaactgc
    10441 caataagtaa accactagta ttgcaaaatc tatccaagta aatcctacca tttgactaat
    10501 cctcccatac taaaagtatt ttattaaaaa tactttctgt atatttcttt tgctcttgat
    10561 ttcatttcat cagttgcttc tttcataggt tgtctacagt atccagcctc aacaccttgt
    10621 tcttcaagta gtaatttaat tgtttgatat aagccatttc ctaatatatc tgttattaaa
    10681 tcattagtca catgttgtac ttcaagtgct tctgatattt tttcattctt agttaattcg
    10741 aatatttgtc tagctctgac accgtttaca ttgaatgtag aaccaattgc cccatctaca
    10801 cctaaaactg tagctggtaa catcatttca tcaaatcctg caaatattaa tttatttggg
    10861 aatgtttttc tcattctctc taataagtag aaatctgctg ctgtaaactt aacacctatt
    10921 attttttcat tttcaaataa ttctccaaac tggtctaaac tcatatcaac accagttaaa
    10981 aatggtatag agtatattat taatctatta tctacagaat ttattattgt attataatag
    11041 tgttttattt cttcaaaatc aaatttatag taaaaaggtg taacagcact tattgcatca
    11101 tatcctaaat cagttgtaaa tttagcaagt tctacagctt cttttaaatt aactgaacca
    11161 acttgagcta ttaattttat ttcttctttc acttcatctt tagctatttc aaatattctt
    11221 ttcttttcat cagttgaaag catgaagttt tcccctgtac ttcctcctac ataaagacca
    11281 tctactttac aaacatctat attgtgtcta attatttgtc ttaacccttt ttcatttata
    11341 tttccttcct tatcaaatga aactaataat gctgaataaa ttcccttcat atctgttgtt
    11401 ctcatatctt tacctccaat tttaattttc aaatatagtt aaatataata attttttaat
    11461 ttataaatta atttttaatt aatttcaatt gctttaacaa atttttctgt tattaattgt
    11521 ggtcttgtta ttgctgaacc tactactgat gaatgaacac ccaactcaaa gacctttttt
    11581 aaatcttgag gagtatttac ctttccttca gctattacag gtatttctaa atctttaact
    11641 aatctttcca ttaatacaaa atctggacct tctaatgttt tagtatatgg agtatatcca
    11701 gatagtgtag ttgaaacgca atctacaccg tactcttgtg ctttgatagc ctcatcataa
    11761 tttgaaatat ctgccattac tagaacaccg ttttctttta tatactttat aagttctttt
    11821 aagtcttcat tatttggtct aactctattg gtagcatcta atgctatcat ttcacaccct
    11881 gttgttaaaa gttcatcaac ttctttttta gttggcgtta tatatatatc tgaatcttca
    11941 tagtttctct ttataatacc tataacagga agtccagtaa ctttttttat ttcaataata
    12001 tcttcaactc cttgagctct aattccaaca gctccacctt ccatagcagc cttagccatt
    12061 ctccccatta taaaaggact atgaagaggt tcatcttcta aagcttggca tgatactatt
    12121 aatcttccct ttaccttatc tagcattttt taaattacct cgtttctgaa atttattttc
    12181 ttgatttatg tattaataat aacatgaaaa ctttttcttt gcaatatttt ttcatatttt
    12241 tttctcgcat ctatattttt actgatttat tttatatatt agcttgtttt ttacatttaa
    12301 aagaaatttt atttcatttt tttggtgttt attttcattt tatataacta ctgttataag
    12361 tccatttaaa taatttcaat aaaaaaaccc atgtatacta tacataggtt tcccaatatt
    12421 attctaaatc cattataatt tttcttccac agcatggaca aaattcagcc tctgtatcta
    12481 tctcactttc acaataacca caaattataa aatcatcaca ttcattttcc aattcaaatt
    12541 ttttaccaca ttcagcacaa tatttatcat ttacatttcc aacataacca cactcacaag
    12601 ttacattact ttctgccatt tccatttcat taatttccat atttttttca taaattaact
    12661 tatcaagttc tagtaactca tcacagaatt tatcaaaact attatcatta atagaatctt
    12721 ctctaatttt ttgatatgtc agtattccca tttccaagaa tatctttgct ttcttttctt
    12781 gaatatcatc aatctctcta tttaattttg atttttcttt atataattca actttaaatt
    12841 ttccactacc aaactttttc ttaaccttag aaaatttatc atctacttta ttattagtat
    12901 ttccttccac agaaattccc tccaatcata tttaatcagc tgaatttagc ttatatccta
    12961 ttaaagatat cacaaaacta tatacaaatg aaattacaat agcttgtaag actttaaatt
    13021 gcattattaa agttgttcca taattagttg tattaaagaa attaatatta ctatctagta
    13081 taaatgtact aaataaagcc aatattccca tcaaaaatgc ataatatata ctaaatacta
    13141 ttataggtct tatactatct gtattatact taaatcttag cttgtatcca tttaataata
    13201 atattaacat agacaatgct cccattgcat aaaacattaa tttagtatct ccaaataaag
    13261 atgaagaact taaagtaaac attgatactg ttgagtttat tatggttact gaaattccat
    13321 ttgcaaaagc ccacatataa ctagctattt gtgggagtat agtaaataaa tttaatccat
    13381 ttgaataact actcatatct agttcattta agtaactact atcagatata gttagtacca
    13441 atagtattac taatactaaa acataaccta atatgaatgt tcttatagaa tttgaaaaaa
    13501 gagataaata catattttcc ttttcataac tttttttata agtagttatg tatgtacata
    13561 taaaccctaa tacaaatcca tttaaaagaa cactaaaaaa ttcataacta tattctaatg
    13621 catatccata ttctaacata ctatgtgaac tagtttttac attagtaaaa atagataaaa
    13681 tagctaaaac taatccataa aaaattccaa ctcctagaga atttgctaat acagtttttg
    13741 aatctctaca tctttttctc ataaaaatta ggtttgatat taataatacc aaaataggga
    13801 ttataagtaa tatcaacaaa cctatattgg catttataaa cccagaaccc atcatggtag
    13861 acatagatac actaatttga cccaagttga gagctaggat tatatgtgct ggatttatta
    13921 aataactaat atcattaaat tgaatagtta taatagcttt aaatatcaat gcaataataa
    13981 acaatataaa tatggttgta aatgttgtta aaaatacacc tcttttattt gcatgatatg
    14041 aaagctcttt tattttagat ttcaagtcta gttttgtttc aagtggtctt aatgatgcta
    14101 tttcataaag ttcatttcca caaaatttac agtaattatc cttagtatta ttttctttat
    14161 tgcaactaga acaaattttt tcttctttac ttttaagagt gatatggcta ttttttattt
    14221 ttctagttgg ttcacctgtc tttttggaat aattattatg tgaataattc atattattta
    14281 ttttcttttc acccattttt tccctccaag ttttactatt gtaaattatc aaatataact
    14341 atatcatata aaacttaatt ataccttaat tggatatatt tatctatttt atttgtttat
    14401 tgctgttgtt ttttaacagg tcttgatatt taaatagttt gttaatgcga actttattat
    14461 ttatatttta ttatgcttaa tttaaattta ttctattctt tattttcagt agtaaactat
    14521 ctgtaaatct taattataat aaagtattaa tc
//
LOCUS       DAYASV010000093        14523 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYASV010000093 DAYASV010000000
VERSION     DAYASV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.094"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            290..1303
                     /gene="aroF"
                     /locus_tag="ACGI68_002695"
     CDS             290..1303
                     /gene="aroF"
                     /locus_tag="ACGI68_002695"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFH0139122.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            1325..2389
                     /gene="aroB"
                     /locus_tag="ACGI68_002696"
     CDS             1325..2389
                     /gene="aroB"
                     /locus_tag="ACGI68_002696"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFH0139123.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            2389..3702
                     /gene="aroA"
                     /locus_tag="ACGI68_002697"
     CDS             2389..3702
                     /gene="aroA"
                     /locus_tag="ACGI68_002697"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFH0139124.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLTFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            3680..4759
                     /gene="aroC"
                     /locus_tag="ACGI68_002698"
     CDS             3680..4759
                     /gene="aroC"
                     /locus_tag="ACGI68_002698"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFH0139125.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            4762..5958
                     /gene="pheA"
                     /locus_tag="ACGI68_002699"
     CDS             4762..5958
                     /gene="pheA"
                     /locus_tag="ACGI68_002699"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFH0139126.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRLIDNDGEQKIEFSLDS
                     SNKKFDLSNVNRYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSKFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            6005..6814
                     /gene="aroE"
                     /locus_tag="ACGI68_002700"
     CDS             6005..6814
                     /gene="aroE"
                     /locus_tag="ACGI68_002700"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFH0139127.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            6826..7359
                     /locus_tag="ACGI68_002701"
     CDS             6826..7359
                     /locus_tag="ACGI68_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFH0139128.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            7380..8219
                     /locus_tag="ACGI68_002702"
     CDS             7380..8219
                     /locus_tag="ACGI68_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFH0139129.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            8487..9968
                     /locus_tag="ACGI68_002703"
     CDS             8487..9968
                     /locus_tag="ACGI68_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0139130.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            10001..13435
                     /locus_tag="ACGI68_002704"
     CDS             10001..13435
                     /locus_tag="ACGI68_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFH0139131.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDKKKALESIQHQLNTKGIAVYQHRIRRRNGNIIWVSIKGRLIVDDGIEKGIWILNDI
                     TDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNVP
                     YSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDED
                     GNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESWQ
                     KPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRRM
                     NQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYNK
                     GTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPIF
                     RKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGIS
                     FVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADKC
                     EKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDRP
                     CNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            13453..14199
                     /locus_tag="ACGI68_002705"
     CDS             13453..14199
                     /locus_tag="ACGI68_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFH0139132.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQGKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(14148..14456)
                     /locus_tag="ACGI68_002706"
     CDS             complement(14148..14456)
                     /locus_tag="ACGI68_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFH0139133.1"
                     /translation="MNFFKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIVKYKT
                     SYKQLLVNPNVEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
BASE COUNT         5993 a         1439 c         2440 g         4651 t
ORIGIN      
        1 tactatgaaa ggaatattaa aagaactaac agcaaaactt tatagctttt tttactttat
       61 gttctggggc ttttatttta gcgctggcta tttcttttac aaaaatatat gtacttcctt
      121 tctggtgagg ccttatttta gtgattttga gcataagtaa attttataaa aatgattatt
      181 ttatatattt atataagatt tagtgcttga tagattattt tagagaactc attagtgagt
      241 tctctttttt attttaaata taaaattata atattgggag gtagtaaaaa tgatagtagt
      301 attaaaaatg ggagcagata aaaatgaagt taaaaaatta attgaggcta ttgggagaga
      361 aggagtagaa gttaatccca tagatggaac agaattaact gttttaggtt tagttggaga
      421 tactagtaaa atagatgcca aaagaataga agcaaataag gtagttgaaa aagttatgca
      481 tgtggtagag ccttttaaaa aggccaatag aaaatttcat ccagaacctt caatcataaa
      541 tgttaatgga atggaaattg gttcaaagaa aattgctatg atagctgggc cttgttctgt
      601 tgaaacagaa gaccaaatag tatctatagc taaagatgtg aaaaaatcag gtgcaggatt
      661 tttgagaggt ggagcattca aaccaagaac atcaccatac gctttccaag gattgaaata
      721 tgatggattg gatttattaa aaaaagcaaa agaaaaaaca gggcttccta tagttacaga
      781 aataatgtca acacaagata tagatatatt tgaagaaaat gtagatgtaa ttcaagttgg
      841 agctagaaac atgcaaaact ttgatttatt aaaagaatta ggtaaaacaa ataaaacaat
      901 acttttaaaa agaggattat ctgctacaat tgaagagtgg ctaatgtcag cagagtatat
      961 aatggctgga ggaaatgaaa atgtagttct ttgtgaaaga ggtataagaa cttttgaaac
     1021 atatactaga aatactttgg atttaagtgc aattttagct gtgaagaaac ttagtcattt
     1081 accagttata gttgacccaa gtcatgcagc aggtaaatca tggatggttg actctttatc
     1141 taaggcagca attgcagttg gagcagatgg tctaataata gaagttcata atgacccagc
     1201 acatgcactt tgtgatggaa aacaatcgat taagccaaat gaatatgatg aattgataag
     1261 tgaattaaag actatcgcat cagcagttgg tagagaaata taatctataa aaggatggtt
     1321 aaacgtggaa gaaaaactat tagtaaattt aaaagaaaat agctacaata tatttattaa
     1381 aaaaaatata ctaaagaata taggagaaga aataaaaaaa atatattttg gaaataagat
     1441 ttttattgta actgatgaaa atatagcaaa attatacttg gataaagtaa aaaataattt
     1501 aagtgaatct ggatttgaaa ttaaatcttt aatattaaaa tctggtgaag aaacaaaatc
     1561 atttaatact cttcctaaaa tatacgatag tttacttgac tttaagttaa ctagaaaaga
     1621 tttgattatt actttaggag gaggggtaat aggtgattta ggtggatttg cagcagcaac
     1681 ttttttaaga ggtataagtt ttattcaaat tccaacatct ttattagcac aagttgatag
     1741 ttctgtaggt ggaaaagttg gagttgacct tgagagaggc aaaaacttag tgggaagctt
     1801 ttaccaacca aaggctgtgt ttatcgaccc aaatgtatta ttatcacttc cagaaaaatt
     1861 ctatagagat ggaatggcag aagttataaa atatggatgt ataaaagaca aaactttttt
     1921 caataaactt ttaagtttaa aaaatagaga agaagtaatg caacatatag aatacattat
     1981 acataaatgt tgctttatta aaaaaactgt agttgaagct gatgaaaaag actttggaga
     2041 tagaatgctt ctaaactttg gccatacatt aggtcatgct attgagaaat actacaattt
     2101 tacaggttat acccatggag aagctgttgc cataggaatg tataagataa ctttagaaag
     2161 tgaaaagata ggaattacaa aaaaaggtac atcagaactt ataaaatcaa ttttaatgaa
     2221 ttataatttg ccttatgaga ttgaattaag agataataaa tctatagtag atgctatatc
     2281 tcttgataaa aaaaatctag gtgatacatt aaaaataata cttttaaaag atattggaga
     2341 gagtattata tataatacta cttctaaatt ttttcaggag gtaagataat gggaagtcta
     2401 aaaatatatc caagtaaatt atctggagat gtaaaaatac caccatcaaa aagtatggca
     2461 catagggcag ttatttgttc atctctaagc aatggaaaaa gtagaatttc taatatagat
     2521 ttttctgatg atataatagc tactataaga gcaatgacat cattaggagc tattatagaa
     2581 aagaaagaag atattttaga aatttcggga attttttcta aagaaggtat tttaaataga
     2641 gagaatcagt tgaatcaacc taaattgaca atagattgta atgaatcagg ctctacattg
     2701 agatttttag tacctatatc tttaacattt gatggtgtaa aaaggtttat aggtagaggt
     2761 aatcttggaa aaagaccact tgatacctat tatgagatat ttgacagaca gaatataaaa
     2821 tatagttaca aagaaaatca gttagatttg attatatcag gtaagcttaa accagatgaa
     2881 tttagagtaa aaggaaatat aagttctcag tttatcacag gtttactgtt tatattgcct
     2941 acgctagaaa gtgattcaaa gataataatt actactgagc tagaatctaa aggatattta
     3001 gatttaactc tttcaacgat aaaagatttt ggggtagaaa ttataaacaa taactataaa
     3061 gaatttatta taaaaggaaa tcaaacgtat aaagcgagag attataaagt agaaggagat
     3121 tattctcaag gagcatttta tttatcagca gatgctatag gagaagatat tagcatatta
     3181 gaccttaaag aagattctct tcaaggggat agtgaagtag ttgaaatttt atctaggatg
     3241 ggaatggaaa tattaagaga aggcaacaaa ataaaaggaa taacaaatgg tttaaatggt
     3301 actttgatag atgcatctca atgcccagat ataatacctg tattatctgt agtagcatcc
     3361 ttaagtatag ggaggactac aataattaat gctggtaggc ttagaattaa agaatgtgat
     3421 agacttcatg caataaatgt tgagttaagt aaacttggag caaatataga ggagaaagag
     3481 gatagtttga taatcgaagg agtaagcaag ttaaatggtg gagtagaggt gtggagtcac
     3541 aaagaccata gaattgctat gacacttgca attgcttctt gtagatgtga taaaccaata
     3601 atattaaaag attttgagtg tgtatcaaaa tcttatccac atttttttaa agattttaag
     3661 atgttaggag gtcgaattga tgagtggaat atggggaaat aatttaaaag tatcaatttt
     3721 tggagaatct catggaaatg ctataggtat aaatatagat ggtcttcctt cggggattga
     3781 acttgacctt gataatattg ataaagaaat gaagagaaga gcacctggaa aaaattctat
     3841 atctacttca agaaatgaaa gtgatatacc agaaattcta agtggatatt ttaatggaag
     3901 aactacagga acgccattat gtgcaatcat aagaaatagt gatactcgtt caaaagatta
     3961 tggagagctt aaaaatttaa tgagaccagg acatgcagat ttcacaggta atgtgagata
     4021 ttctggattt aatgactata gagggggagg tcatttttca ggtaggataa cagctccatt
     4081 agtattttgt ggggctatat gtaaacaaat tttatcacaa aaaggaatag agataggagc
     4141 acacataaaa aaaattaaaa atatagaaga tatgagtttt gattatgtaa atatttcaaa
     4201 acaacagtta tcaaatttgc aaactttgga actaccttta cttgatttat caaaggaaga
     4261 agccatgaaa aatacgatta tagatgcaaa aaatcaagga gattcagttg gtggtataat
     4321 tgagtgtgca gtagtaggta ttaatgtagg gcttggaaat ccattttttg actctgttga
     4381 atctacttta tcacatttat tgttttctgt tcctgctgta aaaggtgtag agtttggttt
     4441 aggttttgaa cttgcagata tgtatgggtc tcaaagtaat gatgaaatgt attatgaagg
     4501 aaatcaagtt aagagcaaaa caaataacaa tggaggaatt ataggaggga ttactactgg
     4561 aatgccaata attttcaaag tggcaattaa gccaactcca tcaattagca gacaacaaaa
     4621 cacagttaat ataaaagata aaaaggacga tattttatat attaaaggta gacatgaccc
     4681 ttgtatagtt caaagggcta tacctgtaat agaagcagta acagccattg gaatttttga
     4741 tttgatgaaa ggacgataat aatggaggat ttaacgagat atagaaatga gattgacaat
     4801 atagataaag agcttattca attatttgaa aagagaatga atacagttct tgaaattgcc
     4861 agacataaaa tgaaaaataa cactactata cttcaaaaag atagagaaga aaaagtacta
     4921 agtagagctg tagataattt agataataaa gcatattcac aagaaacggt acagtttttt
     4981 aattctataa tggaaattag tagaggatta caaaaaagat tgatagataa tgatggtgag
     5041 cagaaaatcg agtttagttt agattcatca aataagaaat ttgatttgtc aaatgtaaat
     5101 agatataagt ctttaaaaga tgaattaaat aaaaaaaata tcctagtagg atttccagga
     5161 aaatcaggtt catttacaga agaagcacta aataaattct tcaacaaaaa aatatcaaaa
     5221 aaacaattta aagaatttga agatgttttc atagcactta aaaataaaga aataaactat
     5281 ggaataatcc ccatagaaaa ttcatctaca ggagctatct ctgaaactta tgaccttttg
     5341 agaaagtacg gtttttatat agttggagaa gaatgtataa aaatagacca aaatttaata
     5401 gggataaaag acacaaagct tgaagatata aaagaaatat attctcatcc acaaggtata
     5461 ggacaatgta gtaaattttt aaaacaaaat agtacatgga aattgatacc atttaataac
     5521 acagcaacaa gtgcagaact tgtaagaaaa cttcaagata aaacaaaagc agcaattgca
     5581 agcaaaaaag ctgcaagtat ttatggatta gaaataataa gtccttgtat aaatgatatt
     5641 gcaaacaact atacaagatt tgtagtaatc agtaatcaaa tacacattga agaagaatca
     5701 aacaaaatga gtgtagtatt ttctgttgaa catgaagcag gaaaacttta taaagtactt
     5761 ggatactttg ctaaaaataa tataaatatg acaaaaatag aatcaagacc tatgaaaaat
     5821 gcctcatgga gatatttctt ttatatagat tttgagtgtt ctatatacaa tgctaaagtg
     5881 tacgatttat tagaattgat agaacataat acagcatatt ttaaatttat gggagtatat
     5941 aaaaataaaa ctgaatagat aagataaatt tttatttaat ttataattat taggaggtgt
     6001 aagtatgaat tttttcggtc tagtaggtga aaaactctct cacagtgttt cacctcaaat
     6061 ccacaagaga gtattcgaga tattgaatat tgaaagtgct tataaaaact ttgaaatttc
     6121 aaaagaagat atatctaagc tagacggtgc tataaaatta ttaggaatac aaggggtaaa
     6181 tgttactgta ccatataagg agcgtattat gaaatatctt gattttatat caccagaggc
     6241 aaaacgtata ggagcagtta atacaatttt acttagagaa aatatgttat atggttacaa
     6301 tacagattac tttggtcttg atagtatgtt taaaatggct aatattgatg ttcaaggaaa
     6361 agtagcagtt atattaggaa ctggaggtgc ttctaaagct gctcttacat attttattga
     6421 ttctggtata gaaaagcttt atgtttcaac tagaaaaaag gatgataaaa aattattaaa
     6481 tagtaaagct atattaatag attatgaaga acttaaacat ataaaaggag atataatatt
     6541 aaatgctaca ccagtaggta tgtaccctaa tgtaggaatt tcaccagtgt caaaatctat
     6601 tatacaaaat tttgatatat taatagattt aatttacaat cctggagaaa cagagttttt
     6661 aagaattggt aatagtatgg gtaaaaaaac ttgtgatgga ttatatatgc tggtggggca
     6721 agcaataaaa tctcaggaaa tttggcaaga tactaaaata gataatagta ttttagatgt
     6781 tatatataat gaactaaaat tagaattttt gtaagggaga ttgttatgat aaataaaaca
     6841 aaagaaaaat tgatattaat aggtatgcca ggtagtggaa aaactacaat tggtaaactt
     6901 ttggccaaag aatataattg tagtttctgt gatatggatg attatataat tcaaatatct
     6961 caaaaaagta tagcagagtt gttttcagaa ggagaagata tctttaggaa ctatgagact
     7021 caagcatgta gagaactttc tataagtgat aaaacagtga tatctacagg tggaggtgta
     7081 ataaaaaagg atgttaatat ggaaatacta aaagaaacag gtataataat ttttattgat
     7141 agacctatac aaaagatttt ggaagatata aatattaatt caagaccatt attaaaaaac
     7201 ggaaaagata gattgtataa tctttataat gagagaataa atctttataa aaaattttca
     7261 gatatagaga ttttaaatga taaatcatta aataatgctg tatataatat tacaaatgca
     7321 gtcagtgaaa attttaaatt tgactttaaa gaaaaataat ctagggggaa ataaaaataa
     7381 tgaatattgt tattgtagga ctaggagtta taggaggctc ttttgctaaa gcattaaaaa
     7441 aagctggata tgaaaatgta tttggagtag atgtagattt agaaacttta aaaaaagcag
     7501 aaaaagcaaa aatcataaaa aaaggatgta ctacaggtaa agagttattt aaaaaagctg
     7561 atttaattat attatctata tatcctagat tagttgtaga ttttctaaat aataataaaa
     7621 acttttttaa aaaagggact ataattactg atactacagg aataaaagaa acacttatca
     7681 atgatgtgtt gcaaattatt ccagatgata tagattttat atttggacat ccaatggcag
     7741 gtagggaaaa aaagggtatt gattttgcaa gtgaacaagt ttttaatggc gcaaattata
     7801 taattactcc aactggcaga aacaacataa aaaatttaga gttagttgaa aatttaattt
     7861 tagagattgg atttaaaaga gtaaaaaagt taacttcaca aaaacatgat gagattatag
     7921 cttttacttc tcagttacct catgttatgg cagttgctct tataaatagt gatgaagaag
     7981 gtagagatac gggtaaattt attggagata gttatagaga tttaactaga attgcaaata
     8041 tgaatgaaga cttatggagt gaactttttt taggaaatag agataattta ctaaaagtta
     8101 tagaaaactt tgaatcagaa gtaaatttag taaaagaagc tatatttaac aatgataaaa
     8161 ataaattaat agagtatttt aaaaaatcat caataagaag agaagcttta gaaaaataaa
     8221 tattaatatt taagctatat atagatgggt ataaatataa gcgatagtgt atttaattgt
     8281 attaataaat atggattcaa tataattaaa tatgctattg atatatttgt acccttaact
     8341 attgttatct tttaaatgat aatgatattt atttaaatac tctttattaa caatttataa
     8401 aacttaattg taaaaaatac ttattaatag taaaataaag tgaatcaatt atattataat
     8461 tgctaaagag agtgaggtgt agctatttga aaaaaaatag attaatagca aagaatatat
     8521 caatagcatt tatagtacta ttctttttta gtgtatttac atttttttat gtaggtaata
     8581 ttaatagagt gttagaatat gaaacaaatg acataataac agttacaatt gctggatgga
     8641 ttatactttc atttttattt ttaggtatta taatttacat attatattca aaagcaaata
     8701 gtcaaaaaac tatagaaaaa gtagcatata cggattttgt aacaggttat tccaactgga
     8761 gaaagtttga attagatgtt acaaatttat taaagaaaac atcacaaaat aataaatatg
     8821 caatggtcat attcgatatt gataaattta aagcaattaa tgatatatat ggccataaaa
     8881 agggaaattt aatattaaaa gatattgcag atactttaaa tgaattgact gatatcaatg
     8941 aaacttttgc tcgtgtaagt gctgataatt tcaatatatt attaacatat aacaaaaaag
     9001 aggatataat aaatattatc aaaaagatta tggcaaataa tgaacttgta aacttatctt
     9061 ttggaattta tgaaatcaaa gataaggatt tatctgtaag tgtatatagt gatagagctt
     9121 cacttgcaaa atcatcaata aaaaataata gtgatgtaaa ttttgcattt tttaatgaca
     9181 aattaagaga gaaattatta tttgaagata agatagaaaa agaaatggaa tatgctcttg
     9241 aaagtgggca attcgtaatg tatttacaac caaaatacaa tattaaatta gataaatttt
     9301 gtggaagtga agccctagtc agatggcaat atacagaaaa agaggtcata tatccaggtg
     9361 attttattcc tatatttgaa aaaaatggat ttattagaaa gattgatatg tacatattag
     9421 aacaagcttg taaagaaatc cggtctttat ttgataaagg aatttcacct ttacctatat
     9481 ctgtaaactt ctcaagagtt gattttttta aaaaagattt tattgaaaac atagtaaata
     9541 tatgtgatag atataaaata ccatatagtt taatagaaat tgaaattact gaatctagta
     9601 tgtttggaga tactgatact ctatttaatg ttagtagaaa cctacaggat attggcttta
     9661 tagttgcaat ggatgatttt ggctctggat attcttctgt aaatatgctt aaaaatatac
     9721 cattaaatgt aataaaatta gatagaggat tttttgttga tgataaagat gttgataaga
     9781 gtcaaattgt tataaaaagc atagtttctc tcattaagca attaggaata agggttgttg
     9841 ctgaaggaat tgaaacaaga agtcaaatag aaatgttaaa aaaagcaaat tgtgatattg
     9901 ttcaaggata ttatttttca aaaccattac caattaaaga atttgaaaaa ttagtctata
     9961 aaatatagtc agcgagtctt aaatgggagg tggtgattaa gtgataagag caaaatcgtt
    10021 aaaaaatgta ctgagtattg ttttaatatt tttatttaca ataatttctt tttctgattt
    10081 tgcatttaaa actaatgtcc aggaagaaga aaatagtaaa attaatttaa gagaaatatc
    10141 cctacaaagt tccaatgcta taaaaataaa attagatagt tctctatcct ttattaaaag
    10201 tgtggcaaat atggtttctt attttaatac tatagataac tcaaaaatag ttccattatt
    10261 agctaatata tcaaaagaat ctgactttca aagaatctct attgtatctc ctgatggaat
    10321 aagtcatacc agtgatggca aaattatcaa tgtgtcaaag tatcagtatt ttaagaaagc
    10381 tattaaagga gaaagtaata tatctgaagt tgtgaaatct ccaattgatg gaaaagatac
    10441 ttttgttata gctactcctg tatttaataa gaataacaaa ataattggtg ttttatacgg
    10501 ttcttactac aatgataaat tagctgaatt tatagatata acaagtttta atagtgaagg
    10561 gtacatagat atatttgaat ctagtggtca atttgtttta aaatcttcac atacaaacaa
    10621 actgattaag tcaagtagta acatatatga tatatttaaa aaagcttctt ttgatgatgg
    10681 ttatagctat aaaaaaatat atgaagatac aaaaaataac aagagtggct ttacctcata
    10741 tgaatacaaa ggagaaaatc gttatgctac ttatacaccc attggtgtaa atgactggta
    10801 tgtaatttct gtgataccac aatatactgt aagaagaaac attgataaaa taaatcaatt
    10861 agcattttct cttattttca aggtattctt attgtttatt atgcttataa tatatattat
    10921 ttataaaaac aataaatctc agaaagatat actaaaagct aacaaagaaa tctcatctat
    10981 tacaaatagc attcctggag gagtacaaaa gtgtaccctg ggtgaaacat gcaatattaa
    11041 ttttttaagt gatggattta tagagttgtt tggttataca agagaagaaa taaaaacatt
    11101 gtttaataat gatttttata gtttaataca taaagatgat gttgaaagaa taaaaaaaga
    11161 aatttttaaa caactttaca aaaataaaac tgttgaatta gaatatagga ttataacaaa
    11221 aaatggtaat acagtatggg ttttagataa aggggagctt ataacagaac ctgatggaaa
    11281 agaatatttt tgttgtgtaa ctgtagatat tactagaaca aaacaattag aacaagagat
    11341 ggaacaaaaa aatctagaac ttgaaatgat atcagaaaat gttttaggtg gtataattat
    11401 aacagaattt aatgatgatt ttactataaa atacgctaac tctggttatc tttcaatgat
    11461 tggatataca cgtgaacaat tagaaaatga acttaaatac aaagcttgta atcttgtttt
    11521 ttatgaggat aaaaagaagg ctcttgaaag tatacaacat caattaaaca caaaaggaat
    11581 agctgtatat caacatagaa taagaagaag aaatggtaat attatttggg tttctataaa
    11641 aggaaggctt attgttgatg atggtattga aaaaggtatt tggattctaa atgatataac
    11701 agatttaaaa aatgcagaac aaaaacttaa aataaatgaa gaaagattta gaattgcatt
    11761 aaagcaaaca tcaaacacta tatttgagta tgatataaaa acaaagtcac ttttaaatac
    11821 tagtaaagag cttgataaat ataatattta taaaattact aaaaacgttc catattctct
    11881 agtagaatca ggagttattc ataaagattt tagtgatgat tttataaaca tgtatgaaaa
    11941 tgtaataaat ggagcaaaaa aagcaagttg tgtagttaag atgaaacttc cagatgaaaa
    12001 ttatatatgg aataaagtta ccttgactac tatttttgat gaagatggta atcctgtaag
    12061 agcaattggt atacaggaag atataagcaa acaaaaggaa gcagagataa gatacaatca
    12121 agaaggtaag tatcattcag ctatattgtc ggaagcaatg ataacttatg aaataaatgt
    12181 atcaaaggat acttttatca aaggaaatga aagttggcaa aaaccatttg gaattaagcc
    12241 tgacaataac tattctcaaa tggtaaaaac tatagcaaat agtattgtac ataaagacaa
    12301 taaagaaaca tttttaaata catttttatg tgaaaactta ttggatgcat ataaaaataa
    12361 tgtgactgaa ataaaattag aatatcgaag aatgaatcaa gaaaataaaa tgatttgggt
    12421 catttgtaca atgcacttaa taaaagattc tattacgaac gatataaaag cacttgctta
    12481 tgttaaagat attgacaagc aaaaaaatga agaaattaga ttaaaattaa aagctgaaag
    12541 agatactctt acaggattat ataataaagg tactacagaa aaattgatta atgatttctt
    12601 gaactctgag gaagcaagct taggaatgca tgcattttta attgttgata tagataattt
    12661 taaatctgtg aatgataatt taggacatgt atttggagat aaagtattat ttgaagtttc
    12721 taaaaaccta aaacctatat ttagaaaaga tgatatagta ggtagaattg gtggagatga
    12781 atttatagta ttcttaaaaa atattcagtc ttttgagaat atcaaaaaga aagcagaaaa
    12841 aatatgcgat atatttagaa attcttatac agaaaataaa aagacatata aaatatctgg
    12901 aacagttgga atttcatttg taccagaaca tggaactgta tttgaagaac tttataaaaa
    12961 agcagacata gcactttatt atgcaaagag caaaggaaaa gataagtatg cactttataa
    13021 tgattgtatc taccaaaatg aacttgaaaa taatataagt gataataata taaaattagc
    13081 agataaatgt gagaaatcca aaccagtact taaagaaatt cttgattcta ttgataatta
    13141 tatctatata gtgaatccaa atacttatga acttcttttt gtcaataata aggtgtctcg
    13201 tttggattta gagataaaat taggagaaac ctgttataaa tcactattaa aatctgatag
    13261 accatgtaat aattgccctc ttaacggatt agaagaaaat aagcattcaa aattcaaaat
    13321 agagacaaat acatttggta aaaatatcag tatagcaagt tggattaatt gcatagatgg
    13381 agaatacaac tgtcttatag agtgtataga ttcttcttta tacaaaaata aataaaaaag
    13441 gactatgaag atatgttaaa tagtataact ttgatacttg gtataatatt tacaatttac
    13501 tatttttatc tcaaaatagt ttttggagga atttcattta gtgagatttt cttagttgta
    13561 ggaattgtac taattatcta tcaaatattt aagaaaaaaa ttaaagaaaa gaaggttttt
    13621 tatagggtgt taaaaataat aatcagcatt tttttaatag tatttgtagt tactgagagt
    13681 ctaattattt tttatcccaa aaatagttta gaatcaaaaa gtgattatct cttaatttta
    13741 ggagcttctg taaaaaagac tacaccaagt acaaccctga agggtaggtt agacactgca
    13801 ttgaaatacc taaaaattaa tgataattgt tatgtggtag tatctggtgg taaaggaagt
    13861 ggagaaaaaa taactgaggc aaaagctatg aaagattatc ttataaaaaa tggtatagat
    13921 aaaaatagga taatagaaga ggataaatcc acgaatactt atgagaattt taaatattca
    13981 aaattgaaga tagaagaaca tagtcaagga aaactaagcg atttaaaagt aaaaatagta
    14041 acaacagatt ttcatgttct cagaagtaaa atattagcat atagaaatgg atataaaaat
    14101 acgtcttttt atgctagtaa atcaaaactt tcatttgtac caacttacta tacaagagaa
    14161 ttttttgcat tatggaaaac aatcattttt gatagataat aaataaaaag actagatata
    14221 aactcatatc tagtcttttc tattccctta attctaatcc attttccaac tttacttgtt
    14281 gtagaaattt caacattggg atttacaagt aattgcttat aagaagtttt atattttacg
    14341 attccaaaat ataattttcc ttcataatcc attataaatc cgaatggtct tactcttggt
    14401 ttattatctt caactgtagc aaaatagaat gttgagtttt ctttaaaaaa attcattatt
    14461 ttttcatgat taaatcctct ttgtatctta tactaatata atgaatataa ataaaatatt
    14521 cat
//
LOCUS       DAYASV010000094        14521 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYASV010000094 DAYASV010000000
VERSION     DAYASV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.095"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(27..278)
                     /locus_tag="ACGI68_002707"
     CDS             complement(27..278)
                     /locus_tag="ACGI68_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139134.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            367..1098
                     /locus_tag="ACGI68_002708"
     CDS             367..1098
                     /locus_tag="ACGI68_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFH0139135.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            1157..2068
                     /locus_tag="ACGI68_002709"
     CDS             1157..2068
                     /locus_tag="ACGI68_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0139136.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIEAGKIELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            2119..2931
                     /locus_tag="ACGI68_002710"
     CDS             2119..2931
                     /locus_tag="ACGI68_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFH0139137.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            3040..3732
                     /locus_tag="ACGI68_002711"
     CDS             3040..3732
                     /locus_tag="ACGI68_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0139138.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            3963..4760
                     /locus_tag="ACGI68_002712"
     CDS             3963..4760
                     /locus_tag="ACGI68_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139139.1"
                     /translation="MSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDNKK
                     NEEISNNKNLNNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENN
                     KYTSKEKNSEDIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSI
                     KKDVTNARNKCKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALK
                     LINTKSPKYIGKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            4797..5954
                     /locus_tag="ACGI68_002713"
     CDS             4797..5954
                     /locus_tag="ACGI68_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFH0139140.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            6106..6666
                     /locus_tag="ACGI68_002714"
     CDS             6106..6666
                     /locus_tag="ACGI68_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0139141.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            6694..7185
                     /locus_tag="ACGI68_002715"
     CDS             6694..7185
                     /locus_tag="ACGI68_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139142.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            7414..7965
                     /locus_tag="ACGI68_002716"
     CDS             7414..7965
                     /locus_tag="ACGI68_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139143.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            8051..8983
                     /locus_tag="ACGI68_002717"
     CDS             8051..8983
                     /locus_tag="ACGI68_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFH0139144.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            9038..9901
                     /locus_tag="ACGI68_002718"
     CDS             9038..9901
                     /locus_tag="ACGI68_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFH0139145.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFTNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            10148..12010
                     /locus_tag="ACGI68_002719"
     CDS             10148..12010
                     /locus_tag="ACGI68_002719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFH0139146.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            12013..13479
                     /locus_tag="ACGI68_002720"
     CDS             12013..13479
                     /locus_tag="ACGI68_002720"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0139147.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASAIATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            13503..14339
                     /locus_tag="ACGI68_002721"
     CDS             13503..14339
                     /locus_tag="ACGI68_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0139148.1"
                     /translation="MNYIIKKVLNSSVVLVHDAKYNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT         5723 a         1414 c         2571 g         4813 t
ORIGIN      
        1 tttaatttta gaattatttt tgataactat ctttttttat ttataaattt tgctcctaaa
       61 tattttttga tatcttcttc aggagaaaag tcatcaataa gtatttcttt cccagtgtct
      121 tcaaatacta caagaacttc acagtcacct tgtaagaaaa attttctaac tgtatcttct
      181 ttaacttctc taacttttat tacattcttt ttttcttttt caaagtcttt tgtgtaataa
      241 cttcccatca tattaattat ttttgtatat ctattcataa aatacctcca tatgcgatta
      301 aatttaaaaa atatgttata ataattgttt gtattaactt aataattata acaaggagta
      361 ataataatga ctagtataaa aaataaagat attttaaatt gtatagatta ttccattaaa
      421 aataagctct ttgacaaact aaatgatgta tataagtcct tgccagtagg aaattgttct
      481 ggatgtggaa attgttgtat ggagtcagta ggtataaatt taattgagtt cttgaatata
      541 ttttgttatc ttgaagatag agttgattta aaaaaaagct gtataagtaa aatattagat
      601 tattattttg aggagtatac aagaaagaac ccatgtccat ttaaagatac taataataga
      661 tgtttaatat atgaagttag accattaaat tgtaggatgt ttggacattg gaaaaaagag
      721 gattataata aaaatttaga taatgttatt gagaaaaata ataactatag agagttaatg
      781 aaaaagcaat atggatttga aataaatgat gaagtagtaa attacaggat agattattgt
      841 gatagattta ttccaaataa agattattta agtaaatcta agagattaag cttttttgat
      901 gagcttatga tattagactc aaatatttat tcaagtggaa atatagatat agactttagg
      961 gatagaggta tagttgaata ttttatagag tcattacttt atagaaattt agcatataat
     1021 gtgaaaataa ggatatctaa agaatacaaa ataagaaaaa gaactataga tagaataaaa
     1081 aggattgttc ttgtatagtt ttatattagt aagtaataaa ttgtatcata aatattgtca
     1141 aaatgaggtg aagaaggtga atcttacaaa agtaaaagga aatacctttt ttataaaagg
     1201 tggcacgaat acaggaatat atgtatttga agataacaca gcacttatga tagacccagg
     1261 gttatgtggt tcaagaccta ggagagtaaa taaagtatta gatgaaaata atatacaggt
     1321 tagatatatt ataaatactc atggtcatga tgaccactat ggagcttgta atcaatttaa
     1381 agaatattat aaagatgtgt gtataatgtc atctgaatac gataaattat atatagaaaa
     1441 tccagaattg ttttcaaagt atattgttgg gggaaagagc aatgtattta tggataatat
     1501 cttaaaaaat aaaatgttgg atgatataaa aatagataaa tctatagaag ctggaaaaat
     1561 tgaattaaac aatgaaatgt ttgatataat tgaatttcag ggtcatacac ctggtagtat
     1621 agctgttttg acgaaggata atgtgatttt tgttggcgat ttattgatag gagatgaaat
     1681 tctatcaaaa tacgatttct tgtttctata tgatatacaa gaacaaataa attcattaga
     1741 aaaattaaaa aatatagatt ttgagtactt agttttaggg catggcaagc aagttatatc
     1801 gaaagaagat tcttataaat taatagaaaa acatttgaat gctttaaaaa aatatttata
     1861 tcaaataaga gagttgcttg tagaaccgaa atctttagaa attttattaa aaaacattat
     1921 aaataacaat aatttaagca ataattataa agagtatcat ttttttaaat cttcgttagt
     1981 atctgtcata agttacttag ttgatttaga agaaataggg tatatattgg aaaatggaga
     2041 attgctttac tatacaaaaa caaaataaat tatgataaaa taaagaaggt cataaaaaac
     2101 aaataaaggg tgagcaatat ggaatacgaa aaatggaatg agatactagc accttatgaa
     2161 catgcagtag aggaattgaa aataaaattt aaaaatataa gaaaagaata tttagcaaaa
     2221 ggtgaatatt ctcccataga gtttgtcaca ggtagaacaa aaaaaatatc ttccataata
     2281 tctaagttaa aaaggataaa tgcaaaagat atagaaactg aaattgatga tattgctggt
     2341 ataagaataa tgtgccagtt tgttgaggat atatatgcta tagttgattt gataaaagta
     2401 agaaatgata tgactataat aggagaaaaa gattatatta caaattacaa agatagtggt
     2461 tatagaagct atcatgttat aataaaatac cctataaact ccattgcggg ttcaaaggaa
     2521 attatatgtg aaatacaaat aagaactctt gcaatgaatt tctgggctac aatagaacat
     2581 tcattaaaat ataaatatga ccattacata cctgagactc ttgctgaaag gcttagaaga
     2641 gcatctgatg ctgcctttct tttagaccaa gaaatgagtg aaataagaga ggacataatg
     2701 aaagctcagg ctatgtacca aatgaagtct atagctatta gagatacttt aaatagaata
     2761 caagagttat atgacttggg tgatacacat aaagctatgc agtaccaaag aaaacttgat
     2821 agaactgaaa cagataaaaa tataactgaa attttggaat taaaacaaga aatagatata
     2881 ttattagagc aatataagga tactaaggta gatgataaag tagttcagta aaaactactt
     2941 gtagaaaatt tgctaaatat taatggggct gcctcttaat ggaaaataat ttctaaaaag
     3001 agacagccta tttagtgtaa ttaagaaagg aatgatatta tgagtttgta tgctataggt
     3061 gatttacatt tttcaacatc agtcaataag ccaatgaata tctttggaag taattgggat
     3121 ggtcatgaaa agaaaattat agataattgg aaagaagttg ttaaggaaga agatatggtc
     3181 ttagtactag gagatacatc ttggggaatt aatttatccg aagctaaaaa agatttagat
     3241 ataataagca aacttccagg tcagaaaata cttataaagg gaaatcatga ttactggtgg
     3301 acaaccgtta caagtttaaa taagctatat gaagacatgc gttttattca gacaaatttt
     3361 tatgaatata aagattatgc aatttgtgga ggtaggggct ggatatgtcc taatgatgtt
     3421 aaatttgatg agactgatga aaaagtttat aaaagagaag aacatagact tagactatca
     3481 ttagaatctg ctcgtaaaag tggtcactca aaaataattg tgattactca ttatcctcct
     3541 acgaatgata aattggaaga atctttattt acaaagttat ttgaagaata taatgttgaa
     3601 aaggtgattt atggtcactt gcatggtaaa gaatctttta aaatgggatt gaaggggatt
     3661 cgaaatggag tagaatatac attagcatct tgtgactata cagaatttaa tttaataaaa
     3721 gttcacgatt aaaaaacata aattggtaaa tactataaga tacatgatta aaaataaata
     3781 tttgagggat ttcggttttg agaaaggaga cacgacttat gaggtggata ttagttttta
     3841 tatttttata tcttattcct ttagtagtga tattcagaaa ttacaagaat tttaagaggt
     3901 cttgtatcta cagtagtatt tatgtggtat tagctacaac aataatgata agtaatattt
     3961 atatgagtgg acttaataaa ataaaagagt ctatgtatta tcacaattat atttctgatg
     4021 agacatatga tgaaaagtat gtatcaaatt ttaaggaaaa agaaagtact gatttaaaac
     4081 aggataataa aaagaatgaa gaaatatcaa ataataaaaa tttaaacaac aaacaagata
     4141 gaaatataca aaataaagaa agtaatgaag aaggaagtaa tgatataaaa gaaaagaata
     4201 attctgatag taatgtttca gtgaataata aatcagaaaa acaagaagat aatacaaaag
     4261 aaaataataa atatacaagt aaagaaaaaa atagtgaaga tataaaaaaa atagatatgg
     4321 aaagtatagg aatttttaag aaagaaattt ataatataga aagagcagct ttagttccaa
     4381 tgagagaatg tataccttac actaaggata tagcaaaaaa tataactaaa ttatctagta
     4441 taaaaaaaga tgttacaaat gccagaaaca agtgtaaaga agttgtaaaa gaatatgaaa
     4501 atatgcagat tccagaactt tctaaggaga agtatacaga aacattgagt aatgctagag
     4561 atgacgtaaa gaaagcatat gagcttagag aaaaatctat ggaaagtgct ttgaaattaa
     4621 taaatactaa aagccctaaa tatataggga agataacaga atatcttgat ttatcagata
     4681 agcagataga aagttttaaa aatagattga acgatttaaa agaagaaata aataattcag
     4741 aaacaaataa tattaattaa taagatttaa agatataagt taaaagggag attatgatga
     4801 ataaacagag tataggaaaa ctaaatatgt tttttatggg gattactcaa agaaaccttg
     4861 ataataggga tttttttatt gagttagagg tgatttttaa atctggaaga aaagagttta
     4921 aaggaattgc aatagaggaa gataataaat ataaatttaa ttttaaagga aaatcagatt
     4981 tatatacgtt taatgagttg cttaagaata tttctaaaga ggcagaaaat tatgatggat
     5041 tagtatttaa atatgttgag cgaggaacta ttgttgtaat tgaaggagac aataagaagg
     5101 taaatgtaaa atatttagat aataaggaag aagtacctaa aatagatgaa tttacggcat
     5161 ctcaaataaa aaacagagat tattatgtaa aagtaggaca agcaaatgca cttttaaaag
     5221 aaattggagt acttacaaaa gatggtaaga taaaaaatga taaaataaga aaatacaatc
     5281 agatagacca ttttgttgaa ttgatagata gtattttaaa agaaataaag gataaagact
     5341 gtataactat cttggattgt gcttgtggaa aatcttactt aagctttgtc ttaaattttt
     5401 atataaaaga agtattgaag aaaaactgtt attttatagg aatagattat tcagatgtag
     5461 ttatagaagc atcaaaaaat atggctaaaa acttgggtta taaaaatatg tcatttataa
     5521 aagaggattt gactaattat acacctaata gggatgttga ccttgtaata agtctacatg
     5581 cttgtgatac agctacagat atggcaatag gtctaggaat tagagcaaaa tctgaagcta
     5641 tagtagtagt accatgttgt cataaggagt tattagggca atatagatat gaagctatgg
     5701 aaccaatatt aaaacatggt gtatttaaag ctagatttgc agatttaata actgatggtt
     5761 taagaacatt gttattggag ggcaatggat atgatacttc tgtagtagaa tacatatctc
     5821 cacttgatac accaaaaaat ttaatgataa gagcgataaa aactaaaacg aataatgaca
     5881 aagcattaaa agaatataaa gaactgaaat cacaatttgg agtggaacca accttagaaa
     5941 aacttatata ttagttaaaa atgtaaaatt tacgaaaagc ttgcttaaat atgcaagctt
     6001 tttggtatta tataggtata gtcatatcca gagttttgat ttataattaa agctgtgcta
     6061 taatatttga ttaatcaaat caataaatgt tacatggagg taaaaatgaa caaattgaat
     6121 atatgtattg atatagatgg aacaataaca agcccttatc attttcttcc atacctaaat
     6181 gatatcttta ataaaaatat aactgaagca gagtgtataa cacataattg ggaagaattg
     6241 tatggttcgg gaataagaga tgtgtatgcg gagtttaata ataagtatat acactcatat
     6301 gatgaagcaa agattgtaga gggtgctaca gaggtaatta gcacattatc taaaggacat
     6361 aatttatctt ttgtaacggc tagacatgag tgtttaactg atgtaactaa aaattggcta
     6421 tcgagacaag gattttcaga tatagatgtt tacttattag gaagtgatta taaagttgaa
     6481 aaggcaagag aactgagttg tgacatattt atagaagata atcctttaaa ctcagtgcaa
     6541 ctagctgatg atggtgttaa agtaatactt ctggatacta actataataa agatgttaaa
     6601 catcaaaata tagttagagt tagtaattgg atagatattg aaaagattat aaaacaaggt
     6661 atttaaagat ttgtagattg aaagggggag catatgatag atttaaaaat tgaaaataga
     6721 aaaggaaaaa tccatgtaaa gagcagtgaa ataaaagaca ttttagaatt acgtccagac
     6781 ttcgaatatg tacaagatat atcaaatact ataaatcaag aaaatatatt ggtatttgat
     6841 tgccagttga caggaaattc ttttgatatg gatgatttag atatagaaga aatattggaa
     6901 gagttaggtg aagaaataga tgagtcttat ttcagtgttt tatttgaaga tgtaagagcg
     6961 tatttaaaag atgctactga tgagatagaa gcagacttac aggataatta cttatttgat
     7021 aatttgagat gttattttga tatatacaat ataaatcaag aatttacaga ttttaagttt
     7081 gtatttttag tttcttttaa agatataaaa atttcttcat tagcaaacct agctaagatt
     7141 gtttctaaga gacagcttat aggtgcatct aagttttata gttaaaatga aatattatta
     7201 taataaaagc tagtaacctt gtaaaaatat aggttactag ttttttattt attcattttc
     7261 acaaaatttc agaaaactat ttacattcta aaaaaagtaa tgtatattaa acataatgta
     7321 cattataatg gataaaaagt ttctattaaa ttactaagtt tatttaataa tcaaaataat
     7381 ttatcttata ttagtatgta ttagggggta tttatgcaaa gtttaaatga aataattgct
     7441 gaaaatttaa aaaagataag gaaagaaaaa catcttagtt tagacaagat tgcacaactt
     7501 agtggtgtaa gtaagagtat gctatctcaa attgaaaagt gtgaggtaaa tcctactata
     7561 tctactttga agaaaataac taatgggcta aaaatttcat ttacatcatt gatggagagg
     7621 caggaaagtg acatagaatt gattcaaaaa agtgatatag accattttat tgaagataat
     7681 ggaaagtata taagttatcc aatatttcca tttgactcca aaagaagatt tgagatattt
     7741 atgattgaaa tagaagaagg tggaaattta gattcaaatg ctgaacttcc aggaacacaa
     7801 gagtttataa ctgtttttag tggcgaagta accattaaaa taaatggaga agattacatt
     7861 gtatctagtg gaaattctat aagatttaaa gctgatgtat cacatatata tagaaactca
     7921 ggcaaaggta tagctaaaat gagtatggta gtatattata tataaataat ttaatggaat
     7981 tagagtgata tttagatgat tttaggtatg aagatatatt atatttaagt tataggagtg
     8041 tgatttatgg atgaataata aaaaagcagt ttttttcttt tgcagtgatt taaagaaaga
     8101 cccagtagcc agtaatgtga taaaatgttg tgaagaaata atggatttaa atcaaactga
     8161 tattgttatt gatgataatt ttgtattaga gtttaaggat aaaaacaata acttatttca
     8221 ttttgtaaaa acaaaagatg taataagtca taattataaa tattatcttc caattttaaa
     8281 tagacatttt agagattatg attttgcagg agttgtaaat tggcatgagg gagaaaatgc
     8341 tccagaacat atcctaacag ctcatacaat tggagatgtt ccaacaggag aatttggaaa
     8401 ttcaaatcca agatacttta aaaatctaat caatgctatt gaggatataa aaaatgaaaa
     8461 tctacttgat gaatttacta cattaacaga agcaactcac tggtctggaa caacatatgg
     8521 tgaagaatct aaattaatta cagaatattc tgtacctatg ttggatattg agataggaag
     8581 tagtagtgat agttttaata atagtatagc aatacaggta ttggcaaaat cattaattag
     8641 agtatttgat tgtgatgaac ctttgaagac tttgttatgt gttggagggg tacattttga
     8701 gaaatctttc tcagatatta taaaaaataa agaatataac atatcaattg gacatgtatt
     8761 gccaaatcaa tggattgtaa gtggaatgta tgatgatgaa tctggatttg agaaactaga
     8821 aaagtgtata aatagcattg aaggaggaat tgattgtata gtttttcatg ataaattaaa
     8881 aggtacctat aaagagcaat gccgtaaact aggtgaaaaa cttaatgtac ctgtatttaa
     8941 acataaaata ttaaaaaatc caaaagactt acctatatgg tagtcttata cagttaatat
     9001 aacaataagt cggattaatt tttaagcgag gtcaaaaatg atattatctt attttgcatt
     9061 attaattaga gtagtgctat tagcttttga aagagtagtt gtaagattat taggtgatga
     9121 agaaggagat atttataaaa atatagcttc atcatttcta tttttcttta tagggggagt
     9181 atgtttactt ccatttagca taatggatag agttagtgat tggtcgtttt taataccatg
     9241 ttatataagt agtttagtct attcaattgg ttcagtagcg tatgtaacgt ctttagcaac
     9301 tggggaagtt tcacttgtta ctccaataaa tagtttaaat tcattgtttt tactgctttt
     9361 atcagttata tttttaggtg aaggtttaag tttagcaaaa gttgcaggaa tcataattat
     9421 gattggtgga gtgtttatat taaaaaaagt aagttctttt ttaaagaatg tctctgtaat
     9481 ttttaacaat ttaccatgta gattaatgtt tttatacata tttttgcagt cagtaggaag
     9541 agtattggat aaggcttttt ttgtacaagt atctcctata ttatattcaa ctatactgta
     9601 ttttttcgta ggtttaaatt tatttatctt tttagcattt aaaaagaaac aaaaaataat
     9661 ttgtgagatt tttactaata aaaaggggct ttcaatttta agtggtgcta ttaatggata
     9721 ttcttattta gcattattaa ttgctttaaa taatctagaa cttagtatag cagagccatt
     9781 ttctcaagta tctatgataa taactttaat acttgctcat tttatattca aagaaaatat
     9841 aaaagaaaaa ataccaggtt caattttgat actaattggt gggtggctac tgttacttta
     9901 attgacataa aaaatgaatt ataaaggtgc tattagttag tatcagaaaa tttaaaaaag
     9961 tattgacaat tcttttaggc aaatgttaat ataataatac aaaatagtat aaatagaaga
    10021 catgttactg gtaatgcagg caggatcgaa tatattgctt tgatttttca aggtgtttat
    10081 ataggttctg ccttttttgc atcttgaatg gccattcata aaaaattatg gagaaggaga
    10141 ttacaagatg aattataaaa aaactgccat tgacattatt gaacaagttg gtggttcaaa
    10201 aaatatttta gctttagaac actgttcaac tagactacgc tttaagttga ttgatagaag
    10261 taaagtcaat caagatgctt taaaaaaagt acaaggtgtt atgagtatag ttaatggggc
    10321 acaatttcaa gttgtcattg gaaatagtgt tgttgatgtg tatgatgaaa ttttaaaagt
    10381 ttgcccttta gatacaaatg atagtactgt aaaaactgaa gaaaaaataa acttaggaac
    10441 aagatttata gaatttatta ttaatatttt tcaaccactg atttttgcta ttgcaggagc
    10501 tggtatttta aaatcactgt tgatgttatt agcaatgttt aatattttaa aatcagattc
    10561 tactatatat acattattag ttgctatttc agatgcaaca ttctatttcc tacctcttat
    10621 ggtagctgta actactgcca atgtattgaa atgtaatcgt ttagtagcta ttgccgctgt
    10681 aggatactta ttgcttccag caacaacaac tgctttaagt gaaggtgttg aactatttgg
    10741 atttacaatt ccaaacattg cctataatgc acaggttttc ccggctatct tatgtgtgtc
    10801 tttcttggca acaatggaga aaatttttaa caagtattca ccaaaaccaa ttcgaacttt
    10861 ctttgttcct atgatgtctt tggcaattac tgttccaatt actctaacta ttttaggtcc
    10921 tttaggatat aacgttggta caatttttac aaccattatt ttattcttat ataacaaaat
    10981 gggattctta gttgtaggat tacttgctgc ttttcttcca tttatggttg caacaggaat
    11041 gcacaaagca ctaatacctt attcaattaa tacaattgga aagttgggtt atgaagcttt
    11101 atatatgaca tcttcattag cccacaatat ttctgaaagt ggtgcttgtt ttgcagtagc
    11161 cttgcgaaca aaagatgaaa cattaaaaca aacagcatta tctgcaggaa tttctgcctt
    11221 gatgggaatt acagagccag ctttatatgg tattacctta caacataaaa gagcaatgtt
    11281 aggtgttgta ttctcaagtg caatttctgg taccttttta ggattatttg cagtaaaagg
    11341 atttgtaatt gttggaccag ggcttgccag tatgagtatg tttattgacc caaacaatgg
    11401 aaacaatttg atttttgcga gtattggttt tgttattgca atattaggct cgtttattat
    11461 tacactgatt atttggaagg aagatacaaa tacagtagag tcatcaattg gagatgttgt
    11521 tgaagaagag ctagaagaag aaaaagaagt aaatatctta gtaactccag tagaaggaaa
    11581 agtaatagat ttaagtaagg ttaatgatga actatttgca agtaagactt tgggagatgg
    11641 agttgcaatt ataccaacca atggaaattt atacgcacct tgtgattcag aagttgtgat
    11701 gttatttgaa acaaaacatg caattggttt aagaacaaaa aatggggctg agattttgat
    11761 tcatattgga ataaatactg taagtatgaa tggagatgga tttaaaacat ttgttaagac
    11821 aggcgataat gtaaaagaag gagatttatt aattcaattt gatttagaca aaatctcaca
    11881 tgctaattta gattcaactg ttatgattgt aaacaataat ggagcagatt atgcttataa
    11941 agtgttaaac cagtcttatg gcaatgtaaa aaaaggaagt atcttgtttg atgtaaaggg
    12001 aggaatataa atatgagaaa atttccagaa ggatttcttt ggggaggagc tacagctgct
    12061 aatcaatttg aaggtggttg gaatttaggt gggaaagggt ggtctgtatc agatgtagca
    12121 aaggcacact ttgatgcaga tgtgaaagat tataaatcta ataatgaaat cacaacaaaa
    12181 gatatagaag aaggattagc acatcctgaa gatgaagtaa attatccaaa acgtcatggt
    12241 agtgattttt atcatcacta taaagaagat attgctctaa tggcagaaat gggatttaag
    12301 acatatcgaa tgagtattgc ttggtctcgt atttttccaa atggagatga taaagagcca
    12361 aatgaagaag gtttacagtt ttatgatgat gtatttgatg agttgataag ttatggaatt
    12421 gaaccacttg ttactatgtc acattatgaa ccaccattaa atatcgtttt aaactatgat
    12481 ggatggtatt cacgacaagt tatcaatatg tttgttaggt atgtagaaac tatctgtgaa
    12541 agatataaaa acaaagtgaa atattggctt acatttaatg aagttgattc tatgattcgt
    12601 catccgtaca ctactggtgg tttagttcga gaccgtttta aggataaaaa ctttgaggaa
    12661 gttatttttc aagctatgca tcatcaattt gtagctagtg ccattgcaac aaaaatttgc
    12721 catgagatta ttccaaattc aaaagtaggg tgtatgttaa caaaattaac gtattatcca
    12781 tatacatgta gaccagaaga tgttttggca actcaacaaa aaatgagaag tatttatgca
    12841 tatagtgata cgcaggtttt tggtgagtat ccagtatatt tactgtcgta ttttaaaaat
    12901 aataacattc aaatagtaaa agaagaacat gatgatgaaa ttatgaaaaa gtatccagta
    12961 gattttattt cattctctta ctatatgtct tcttgtgagg cagctgatac aactggatta
    13021 gatattacac ctgggaatac gttattagct gtaaaaaatc cttatttaga aatgagtgaa
    13081 tggggttggc aaattgattc aataggtcta cgtatatctt tgattgaatt atatgataga
    13141 tacagaaagc ctttatttat agttgaaaat ggattgggag ctaaagatat tttaactgaa
    13201 gataaaaaag ttcatgacca atatagaatt aattatttaa aagaacactt taaatgtatg
    13261 ttagatgcga ttatcgaaga tggcgttgaa ctttggggat atacttcatg gggttgtatt
    13321 gatttggtca gtgaatcaac gaaacaaatg tcaaaacgtt atggatatat ttatgtggat
    13381 gctgatgact atggaaaggg gacatataat cgatataaga aagatagttt ttattggtat
    13441 aaaaaggtaa ttgaaaataa tagtatagat tttaaatgac caatagaagg tgagcaaata
    13501 aaatgaacta cataataaag aaagttttga actcgagcgt tgttttagtt catgatgcaa
    13561 agtataatga atttattttg ctagggaaag gaattggata tggtaaaaaa acaggagaat
    13621 atatttgtgg ttccaatgat aatcaaatgt ttgtccctgt tgaaaataca aaatcaaaac
    13681 aatttttaga attaatggat gatattcctg tcgacatttt aaagataaca caggaaataa
    13741 tagtagaggc agaaaagtta ttgaactcat cttttaataa aaacttatac ttaatattag
    13801 cagaccattt taactttgca attgaaagga tgcgtaaagg aataaaaatt acaaatcgag
    13861 tattttggga aataaaaaat tattatccaa atgagtttaa ggttggaatg gttgcaattt
    13921 atatggtaga ggagagattg ggcattcaat taccagagga agaagcagct aacattgctt
    13981 tccattttgc aaatgctatg gcttcagatg gaaattcata tgatacgatt aagtatgcca
    14041 aagtaattgg agaaataatt aatatttttg ttttttcttt gaatagaacc ttagataaaa
    14101 agagtatgca ttatatgaga tttataacac atataaaatt ttttgtggaa agattttttt
    14161 cagatagtat gttaagcagt ggtgatgatt tattatttaa tcaaatgaaa aggtcaaatc
    14221 caaaagaaat gttaattgca ttaaaagtta gagatttttt agaaaagaaa tatggaaaaa
    14281 agttaacaaa tgaggaaata gctttcttgg ttgtacatat cgcacgagtt aagcaataga
    14341 gtgaattact aatattatct tatacgtcta catggtagaa aattgaaaga aggtaaagta
    14401 tataaagaaa ctgagacata ttattttgtt gttcattatg tttattttat gagtgtatcc
    14461 tgttaaaaat ttaagttaat atttataatt aaaatataca aatagttaaa ataagtttat
    14521 g
//
LOCUS       DAYASV010000095        14309 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYASV010000095 DAYASV010000000
VERSION     DAYASV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.096"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            100..561
                     /locus_tag="ACGI68_002722"
     CDS             100..561
                     /locus_tag="ACGI68_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0139149.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            629..2875
                     /locus_tag="ACGI68_002723"
     CDS             629..2875
                     /locus_tag="ACGI68_002723"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139150.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            2868..4700
                     /locus_tag="ACGI68_002724"
     CDS             2868..4700
                     /locus_tag="ACGI68_002724"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139151.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            5425..6696
                     /gene="hflX"
                     /locus_tag="ACGI68_002725"
     CDS             5425..6696
                     /gene="hflX"
                     /locus_tag="ACGI68_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFH0139152.1"
                     /translation="MAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSITQN
                     AKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRTAL
                     ILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKLEL
                     DRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLVDI
                     FKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRSTL
                     EEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVLDR
                     ILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNENTS
                     IINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            6947..7426
                     /locus_tag="ACGI68_002726"
     CDS             6947..7426
                     /locus_tag="ACGI68_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0139153.1"
                     /translation="MKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELTMKQWLL
                     LAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAICLVTKD
                     RVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVENNDVGE"
     gene            7476..8000
                     /locus_tag="ACGI68_002727"
     CDS             7476..8000
                     /locus_tag="ACGI68_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0139154.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            8312..9676
                     /locus_tag="ACGI68_002728"
     CDS             8312..9676
                     /locus_tag="ACGI68_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFH0139155.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNAC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            9815..10981
                     /locus_tag="ACGI68_002729"
     CDS             9815..10981
                     /locus_tag="ACGI68_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0139156.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            11098..11880
                     /locus_tag="ACGI68_002730"
     CDS             11098..11880
                     /locus_tag="ACGI68_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0139157.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(11999..12652)
                     /locus_tag="ACGI68_002731"
     CDS             complement(11999..12652)
                     /locus_tag="ACGI68_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139158.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(12676..14040)
                     /locus_tag="ACGI68_002732"
     CDS             complement(12676..14040)
                     /locus_tag="ACGI68_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HFH0139159.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         5543 a         1603 c         2413 g         4750 t
ORIGIN      
        1 atttatatga attttttata aatatattga aaaaatgcat gcagtatagt aaaataatgc
       61 ttagccgact aagtaatata aaaaataagg agggcaagta tgaatagcaa ttctgaagaa
      121 aaattaaatg atgttttctt gcaattaatg cgaatatatt ttataagaac tcatttttat
      181 tttgataaat taggcttata tcatggtcaa ccccaaatac ttttttcttt aaagatgaaa
      241 gatgggcaaa gtcaaaaaga attagcagat aaaaggaaag taaaagcatc tacaactaca
      301 gttatgataa agaggatgga aaaagctgga cttgtggaaa gaaaacaaga tgaaaaggac
      361 caaagagtat ctaggatttt tttgacagac aaaggtttag aaatatgtaa taaagttgaa
      421 gaatttaaca aggaattgga aagagagttt tttttaggat ttagtgaaga agaaaaaata
      481 gtacttagaa gacttctaat gcaagtacga gataatttaa aagcaggatg tcataagcat
      541 gaagatgaaa aatattttta aagtttaaaa taatgttttt caatttaatg ttattagact
      601 aattaatatt acagaaggga agaaatgtat gtttaaatta attaaatatt taaagaaatc
      661 ggcattatca atagtaataa tagtctgtct attggtaatt caagcggtat gtgacctttc
      721 tttaccagaa tacacatcca atatagtaaa tgtaggtata caacaaggtg gagtagaaaa
      781 ctctgttcca tctgtgatta gagagagtga attaaataaa attactttat ttatggataa
      841 aagtagtaag gataaagtac tagataatta tactttgctg aataaaaagg attatgttaa
      901 gtataaagat aagtatccag gtctaaaaga tgaatctctt tatgagttaa atactaaaga
      961 caaagacact atagatgatt taaatgttat ttttggtaaa gctattttaa tagtatcagg
     1021 gcttgagggc gatagtaaag aggtaaaagc tatgaaagct cagttaatga gcaaacttcc
     1081 accacaagct actcaaagtg gagatgttga tatatttaaa ttgttatcag caatgccaaa
     1141 ggaacagtta gatacattga ctaaagagat gagcaaaggc tttgaaagta tgccagagag
     1201 tatgataaca caaagctctg tttcatatgt aaggtcagaa tatgagaaaa taggtattga
     1261 tacagaaaaa actcaaaata attatatatt atttactggt gctaaaatgc ttggtatagc
     1321 aatgattagt atggtagcta caataacagt aggattttta gcagcaagag tagccgcaag
     1381 tgtaggtaga aatttaagaa gtggagtatt tagaaaagtt atgtcatttt caaatactga
     1441 aatgaatgaa ttttctacag cttcattaat tactcgtagt acaaatgata ttcaacagat
     1501 acaaatgttg atggttatgt tgcttagaat agtattctat gcacctataa tggctatagg
     1561 tggagtgata aaggttttaa acacaaatac ttcaatggcc tggattatag cagtagcagt
     1621 agttgctatc ttatcattaa ttgtagtgtt attctcagtg gttatgccta agtttaaact
     1681 ggttcaaaag cttgttgata agttaaactt agtaagccgt gaaataatta ctggtattcc
     1741 agttatcaga gcttttagta ctgaaaaaca tgaagaagaa agatttgata aagcaaataa
     1801 aaatttaact aaaacaaatc tttttgtaaa tagagttatg acatgtatga tgcctgctat
     1861 gatgcttata atgaacctta tcacagttct tatagtttgg agaggtgcat acagtgttga
     1921 ttctggagca atgcaagttg gagatatgat ggcatttata caatatacaa tgcaaataat
     1981 aatgtcattc ttaatgatat caatggtatc tattatgctt ccaagagcat ccgtttcagc
     2041 tacacgtata gatgaagtat tagtgtcaga tataagcata aaagacccag aaaaatcaga
     2101 ggcacaagag tttaaggaag ataaaaaggg tcttgttgaa tttaaaaatg tttcgttcat
     2161 gtatccagac gcagaagaac ctatattaac aaatatatct tttacagcaa agccaggaga
     2221 aacaactgcc tttataggaa gtactggtag tggtaagtca actcttataa atttaattcc
     2281 tcgtttcttt gatgttactg aaggagaaat attacttggt ggagttaata taaaaaatgt
     2341 ttctcaacat gatttaaggg aaaaaattgg atatgtacct caaaagggag tattgttttc
     2401 tggtactata gaatccaact taaaatatgg tagaaaagac gcaacagatg aagaaatgag
     2461 aacagcagct gaaatagctc aagctacaga atttatagat tctaaaccgg aagcttttaa
     2521 aactgaaatt tctcaaggtg gaacaaatgt atctggaggt caaaaacaaa gactttctat
     2581 tgcaagagca atagcaaaaa atccagaagt ttatatattt gatgatagtt tttcagcact
     2641 tgacttaaaa actgatgcag ctcttcgtaa ggctttaaaa tcaaaaactg ctgaaagtac
     2701 agttctaata gttgcacaaa gaattagtac tatcttaaat gcagaacaga taattgtttt
     2761 aaatgagggc gaaatagttg gaataggaac acataaagag cttctaaaaa attgcgaagt
     2821 ttatgagcaa atagctttat cacaactttc aaaggaggag ctagctaatg agtagaatgg
     2881 gaagaggtcc tatgggaaaa tctatgggag caggacaaaa agcaaatgac tttaaaggga
     2941 ctatgcgaaa actaattgca tatttatcta aatttaaaat atcaataatt ctagtaattg
     3001 tatttgctat tggtagtgcg tcgttttcta tagtaggtcc aaaaatttta ggaaaggcaa
     3061 ctacaaagat atttgaaggg ctagtgagta aagtttctgg aggaaacgta ggtatagatt
     3121 ttaatgcaat tggaaaaatc ttaacctttt tacttttcct ttatttgata agtgcactgt
     3181 tctcttttat acaaggattt ataatgagtg gaatttctca aaaagtatcc tataatttaa
     3241 gaaaagagat ttcagcaaaa ttggatagat tgccaatgaa gtattttgat actaagacgc
     3301 atggtgaaat attatcgaga attacgaatg atatagatac tttaaatcaa agtttaaatc
     3361 aaagtatgac acagcttata acatcagtaa caactatgat aggtgttttg ataatgatgt
     3421 tatctataag tggaattatg acattagtgg cagttttaat attaccaata tcaatgtttg
     3481 taatttcaag aataataaag aaatcacaaa aatactttag atatcaacaa gaatatttag
     3541 gaaatgtaaa tggtcaagtt gaggaaactt acagtggcca aacaatagta aaagcattta
     3601 atcgtgagga tgaagtaatt gaagagtttg ataaactaaa tgatagcctg tataattcag
     3661 cgtggaaatc tcaattttta tcaggtataa tgcaaccact tatgatgttt attggaaact
     3721 tgggatatgt aatggtatca atcttaggag gttggcttgc aattaagaaa acaattgagg
     3781 ttggtgacat acagtcattt atacaatatg taagaaactt tacacagcca atgactcaga
     3841 ttgcacaggt tgccaattta ttgcaatcaa cagcagcagc ttctgaaaga gtatttgagt
     3901 ttttagaaga ggaagaagaa gtccaaattg ttgaaaatgc agtttcaata gatggtttag
     3961 aaggtaaaat tgattttgaa aatgtcaact ttggatataa cccaaacaag actataataa
     4021 atgatttctc agtaaatgtt aaacctggtc aaaaagttgc tattgttgga cctacaggag
     4081 caggtaaaac tacaatagtt aaactgttaa tgagatttta tgatgttaat agcggttcaa
     4141 tactgattga tggtcataat attaaagatt ttaatcgtag tgagcttaga gaaatgtttg
     4201 gtatggttct tcaagataca tggttattta gtggttcaat tatggaaaat ataagatatg
     4261 gtaagttgaa tgctacagat gaagaagtta tagaggctgc taaatctgca catgttcata
     4321 gatttattaa gacattacca gatggatata agatgaaatt aaatgaagaa gcaagtaatg
     4381 tttctcaagg tcaaaaacaa ttattgacta tagctcgtgc tattttagct gatcctaaaa
     4441 ttttaatatt agatgaagct acaagttctg tcgatactag aacagaagta ttgattcaaa
     4501 aagctatgga taatcttatg gaaggtagaa caagttttgt tatagcccac agattatcaa
     4561 ctatacgtga tgctgatatg attcttgtaa tgaatgaagg agatatagtt gaacaaggaa
     4621 atcacgagga acttctaaag aaaggcggat tctatgccaa tctttacaat tctcaatttg
     4681 aagaagatga agcaatgtaa caaaaaacta tcttagcata gacatgtaat taataagata
     4741 aaataaatga gggtgcctta aaatgaactt tttaattcat aaggcactct tttgtgtatg
     4801 aaataaagtg tatttttatc attttaacaa tgaaaaagag gattctctat attttgagat
     4861 agccacataa ttttatgtct ttacaaagtg tttattttat tataatatgt agtaaggaaa
     4921 atgttggagg tgattatcaa aatgtatatt taattttatt ctttaaattt tattataaat
     4981 agaatttaat tttaatagtt gatgattata ttgaagtata tgtttttatt acttttatta
     5041 atcatgcaaa taactactta aatttaagaa tagggttaaa tataaaatga taattacata
     5101 taagttaatt aaactaatgt atttttaata tatatttaga attttaatcc ttcttatgat
     5161 ataagtagct tgttttgtat tacttagatt tgtaaggagt ttttttattg aaaaaactgc
     5221 ataatatatt tgggaggagg tgtaagaaag tgatttttta tataagattg agaaaataat
     5281 gcacgattat ggttattata atagtaaata agaagtgtca tttaaggata agataaaaag
     5341 caaaattctt gatgaattta attatagagc cataatctcg tgctatagat taaataaaaa
     5401 atagcagata aggagaatat ggatatggcg aaaaaaggaa taacagtagg attaaacata
     5461 aataataaat ctgaggactt taatgaacta atgattgaac ttgaaaactt atgttcagca
     5521 tgtgatattg atgttgtagg gagtataact caaaatgcaa agcaggttaa tagagcattt
     5581 tacattggga caggtaaagt tgaggaaata ttaaacttaa taaagaaaga aaatatagag
     5641 atagttatat tttacaatga actttctaca tcacaactaa aaaatcttga agaaaagctt
     5701 aattgtgaaa ttatagatag aacggcattg atacttgata tatttgcaca aagggcaaaa
     5761 actagagaag ctaaacttca agtagaagta gcaagtttaa aatatatgtt accaagactt
     5821 ataggttcaa atgaaaattt aggacgtcaa agtggtggag ttggtactaa aaatagaggt
     5881 tctggtgaga aaaaattaga acttgaccgt agaagaatag aagaaaagat aacatcttta
     5941 aatagggagt tggatgattt aaaattccaa agagaaactc aaagaagtat gagaagaaaa
     6001 tcaaatcttc caaatgtagc tctagtcggg tatacaaatg ctggtaaatc ttcaataatg
     6061 aataaactag tagatatatt taaaaactct gaagaaaaga aagtgtttga gaaaaacatg
     6121 ttatttgcta cacttgaaac ttctgtcaga aatatagttc ttgcaaataa caaagaattt
     6181 ttattatctg atactgttgg ttttgtaagt aatctaccac atgacttagt aaaagcattt
     6241 aggtcaacat tagaagaagc atgtgaggca gacgtactgt tacatgttat tgatatttca
     6301 aatccaagct ataaaagtca tattaaagtg acagaagata ctttaaaaca aataggtgca
     6361 gatggtatac ctatgataca tgtgtataat aaaattgatt taatagatgt agaagtattg
     6421 gatagaattt tagatagtat cgataaagaa ggtatatttg tttcagttaa aaaagatata
     6481 aatatagata aaatgataaa atgtatttgt gatagcatat ttaaagatta tgtaagatgt
     6541 aagttcttaa ttccatatga taaaggtcat gtagtttctt attttaacga aaatacgagc
     6601 attataaata cagaatacag agaagatgga gctattttag atgtagaatg tagcaatata
     6661 gaatataata agtataaaaa gtatgcttta gagtaaattt tgaaaaactt attatagcct
     6721 aaaatatatt taatttataa tttattataa attgattgta aatatgattt tatgaaacgg
     6781 tatagccata atgctatgcc gttttgccgt ttataataaa aataaattta ttgacataat
     6841 agtaactaat tactataata taaagtaatt agttactatt taaaattatc aaaaaattaa
     6901 aatctaagat aaagagaagt gtataataat ggatgcagaa agtttaatga aaaaatatat
     6961 ggtagatgat gatagtagaa aacaagctat atttgcaagt atatttgtaa tgcaaaataa
     7021 acttcaaact acatgtgata aattagaccc agaattaaca atgaaacaat ggttactttt
     7081 agctattgcg gactctgtag atgaaactct tacattaacc aaacttggag aattaatggg
     7141 gtgctcaaga caaaatgtaa aaaaactagc tattgcttta gaaaagaaag gttttataca
     7201 aattgagcag aatgaaaaag attcaagagc tatctgtctt gtgacaaaag atagagtgca
     7261 agagtattct aaaaaagtaa gaggtatgca ggaaaaagta ttacagcttt tatttgaaga
     7321 ttttacagag aaagaagtgg aacagttatt ttttggaatt atgaaattaa gtataggaat
     7381 tgaaaatgtg aaaaattatg tagaaaataa tgacgttgga gaataagcaa gtatcaaatc
     7441 aactttgtat gacatcagag agggggataa tttatatgaa aaatgtagta gtgatttata
     7501 agagtaagta tggttcaaca aaaaaatatg ctaaatggct ttcagaatta ttgtcttgtg
     7561 atatttttga aactaagaat attaatatag aaaaggttat gaagtatgat acgattattt
     7621 taggaggagg tctttatgct tgtaatatag cgggaatttc tttcttaaaa aaaaattata
     7681 aagaattgca gaaaaaaaat ttagtagtat ttggcgtagg agctgctcca tatgatgatg
     7741 aagtaataaa gaaattaaga gaagtaaatt ttaaggatga attaaacaaa attccttgct
     7801 tttattgtcg tggggcatgg gatgagacaa aaatgtcttt tatagattat actctttgta
     7861 gtttttttaa aaaatctata gctaaaaaag aacgtcaagg tcttaaacca gcagaagcac
     7921 ctttattgtc tggagaagcc tttgattggt gtaataaaga aaatttaact ccaatacttg
     7981 attatataag agaactttaa gaggtatata tttgtaatta tttattatct aaagttagat
     8041 ttcatagaaa cttactagac attatttata aaataatatt attaaagatg gataatttaa
     8101 ttgtttcaat aaggttataa ttttaaaaaa tgtttatttt aaaataaact attttataag
     8161 cttacttata cttaataagg ggcttttttt attggaagat ttatgttata ttgagatgaa
     8221 tttattatga tataaaaaat aatgttagga taaatttaaa tctttttcta aaaatgtatt
     8281 agtataattc ttaaagaaag gatttttaac aatggatata aaagagttag ttaaaatgca
     8341 aagaaaatat tataatacag gtaaaacaag agatatatcg tttcgtatag aacagttgaa
     8401 gaaacttaag ttagttgtat cacaaaatga agaaaaaata cttttagcac taaagaaaga
     8461 tttaaataaa tcggattttg aaggttttat gacagaggta ggcatgtttt atagtgaatt
     8521 aaactttgct ataaagaata taagaaaatg gagcaaaata aaaagagtta aatcaagtat
     8581 ggttaatttt ccatctatat ccaaagttgt tccacaacct tatggtgtta ctctcataat
     8641 gtcaccttgg aattatccat ttcaattagc acttatacca ttagtttggt cattagcagc
     8701 agggaattgt gttatattaa aaccttctga atattctgtg tcaacatcta gtgtagttaa
     8761 agacatagtt gaagatactt tttcaaaaga atatgttgca gttgttcaag gaagtcaaga
     8821 agaaagtgaa aagttactat tggaaagatt tgattatata ttttttacag gaagtactaa
     8881 tgttggtaaa ataatcatga aatcagcatc agagcattta actcctatta ctttagaact
     8941 tggaggaaaa agtccttgta tcattttaaa agatgctaat atagacctta ctgccaaaag
     9001 acttacttgg ggaaaactaa taaatgcagg acaaacctgc gtggctccag attatgtttt
     9061 agtgcatgaa gatagaaaaa atgagttaat agagaaaata aaatattata ctaataagta
     9121 ttttggggat aatgcatgta ataatgagca atttcctaaa atcataaatc agagacattt
     9181 taatcggata ttatctctta ttgataaaga taagatagtc tatggtggaa attataataa
     9241 ggagacatta aagatagagc caactatagt agacaatgtt aattgggatg ataatataat
     9301 gaaggaagaa atatttgggc ctatatttcc cattttaaca tataaagatt tagatgaagt
     9361 aatacaaaaa attatacaaa tgccaaatcc attggcactt tatattttta caaaaaataa
     9421 gtacctagaa aataaacttt tagaaatgat tccagcaggc ggatgttgta tcaatgatac
     9481 agttactcat atagctacga attacttacc ttttggtggt attggtgaaa gtggtatggg
     9541 ctcatatcat ggaaaagctg gattcgatac atttactcac tataagagtg tgttaaaaaa
     9601 attaaatttg gatgtcccta ttagatatgc tccatacgat aataaattaa ttaaggttct
     9661 taaaaagata atgtaggtta tattgtagaa taataaatat aaataaaatt gagtatttgt
     9721 attctgaata tattttattt ttaatttggt atatttgtta taaaagtggt gattttgtga
     9781 taaaatttaa ttatatagaa ataaggagga aaaaatgaaa attaattgga atacaaaata
     9841 cacaacaata gcaatttata catttataat agctgctagt agtattatat tttatctagt
     9901 atcatcacaa attgatgtat tttctaataa tttagatgct atatttacaa cattacaacc
     9961 ttttataata ggatttgcta ttgcatatct tcttaatttt attttaaagt tttatgaaga
    10021 cagaattttt ataaaaagtg agaaattaaa aaaactaaaa caaagttcaa aaagaggctt
    10081 gggtctttta ttaacatatg caacagctac attgatactt tacttattta tgcatttcgt
    10141 attgccacaa gtaattgaga gtattgttgg actagcaaat gatataccta tgtatgtaaa
    10201 caatgcaact aagcttattg ataaattaat gactgattta aatcttgatg agcaatactt
    10261 taatttggca gttgataagt ggaatgaatt tgttacatat attataaagt ttgtaacaga
    10321 tttaatccca atattaggaa gtatgttaaa gaatgtagcc tctagtatat ggaatattgt
    10381 actaggatta attgtttcag tgtacttatt aattgataaa gaaaaattct atggattaag
    10441 caaaaaaata acttatgcag tatttacaga aaaacaagct gccagaattt tagaattaac
    10501 tcatagaagt aactatacat ttggaagatt tttaggtggc aaaatattag attcattcat
    10561 tataggtatt ctaacatttg taatacttac gttagttaaa atgccatata cattattaat
    10621 atcagttata attggtatta caaatattat ccctttcttt ggacctttat ttggagctat
    10681 accatctacg ctaatagtga tgtttgtatc tcctataaaa gctttctggt tattgttgat
    10741 tatattaata atacaacaga tagatggaaa tattatagga cctaaaatac ttggagattc
    10801 aattggtata tctgcatttt ggattttgtt ctccttattg gttgcaggaa aattattagg
    10861 ttttattgga atggttatag gagttccaat gtttgcagta atttattcta taattaagga
    10921 tattgttgaa agtaaattgg acaaaaaggg attaccgaca gatacttccg attatatgta
    10981 gctaaaaaat attatgtata attaaaaata tataaattaa gcaatattta aagcactgag
    11041 aaagttaaac tcttggtgct ttttagatat aaaattatta tagaagaggt gttttttgtg
    11101 gcatttatag aatgtaaatt taaatctaaa tatactggag gagaaactga tataattgta
    11161 attttgcctg tagcaaatgg aaaagaactt tttagtggta aagagatata tgattttgaa
    11221 ggtaaattta aaacattata cttatttcat ggtttagaag atgaccaaag ctcatggatt
    11281 agatatacta atgtagagca ttatgctaaa aataaaaata tagccctagt aataccaaga
    11341 gtagaaacta gtttttatac tgatatggcg tatggacacc aatattttac ttttgtaagc
    11401 gaggaattac ctaaatttgt tagagctata tttcctttat cagataaaag ggaagataac
    11461 tttgtagctg gtatgtctat ggggggatat ggagctttaa aattagcgct ttcaaaacca
    11521 aaagagtttt cggcagttgc aagtttttct ggttcgcctg atatgattca tgagttagaa
    11581 aatcaacaaa tagaaaccaa tgcagacatt ctatttcacg attttggtag accagaagac
    11641 gctagaaata gtgagaatga tttactttac atacttaaga accttaaaga aagaaatgaa
    11701 gatattccta aaatttatca attttgtggt gatagagatt ttttatataa aaataatcag
    11761 atgtttaaaa aatatgcaga ttctctgggt gtagatgttg aatatgaaga gtgtgtaggg
    11821 gggcatgaat ggcgtctttg gaatttatgg ataaagaaat ttatagatat aattggatag
    11881 tacacttatg gtattaacag gtatactata aaatatcttt aaatatgata aagactgaaa
    11941 tcctaaaatc aaattatgat tttaaaattt cagtctctaa gaatttaata aaattttatt
    12001 aagttcttac acgagttatt attggagtaa aattgtcaac tatatctata tagtatttac
    12061 tcaattcctc taaaatctct tcgcataaat ccgtactatg accaagatta ataagaaact
    12121 gttttgtaaa atgctttatt acagcttgag aagatatatc gtacttgtgt gaagtataaa
    12181 ctctaattat ttcaggtttc atagcaagta taccaataca agaaagtatt aattcatcat
    12241 gttctataga ttctttatca ttaacatatc ttttcatgac catttcggtt acacttcttt
    12301 gatagcttaa aatagcttct tcataagcta tatccaaata aaaatttcta tatgcttcgt
    12361 tttttagaag gaaacaaagg tcagcattca tagcaagaaa gaacatcatc atagtacctt
    12421 cttcatggta gttatagatt aaagactgca tttcatttct tatatcttca tatattatag
    12481 ctcctatttc gcttttactt gagaagtaat agcttattag acctaaattt atattagctc
    12541 tttctgcaat ttgcctagtt gttgtcttat gataaccttg ctcaaaaaat aattctctag
    12601 cagcacttat aatactagct tttgtttctt tgcccttttc tgattttgac atatttactg
    12661 tgtatacctc tcttttcaat ctttttttga aaatttgcta actataaata ttgtaataga
    12721 accacctatt ataggaacca aaattctgtc aaatacagta agaataccaa tcatatttaa
    12781 taatgccaat ataacactta ctataacagc atagatagct cctttttttg tagcaccttt
    12841 ccagcaaaga ccaattacaa atggtgcaaa tattacacct gtacataatg gagctggcca
    12901 aaacaataat tctaataaac tatcaaattt aagtgctatg attgcaccta ttatagctgt
    12961 caaagctgat gcaatttgag aatactttag agtatttttt aaatcattaa attctatatt
    13021 aggatgtaat aatttgttat aaatatctct tgatataata gcactcattg aggtcattaa
    13081 tgaatcggca gtagacatta tggcagccaa tatagcagct aagaataata cagatattaa
    13141 aggtggcaaa gtagtcgata tcatttgagg taaaacctga acagatgata tatttggaaa
    13201 taaactacca ccaatagctc caattattcc tatagggaaa gcaagaaata atagtataaa
    13261 tcctgcaatt acactacttt taactgcatt tttagcatta tcagcacatt gatatcttaa
    13321 aaaactttca taagaaaata ttgagcctaa aactaatggt aaaactgtat acataaaatc
    13381 actaatcgat ttatttcctg gaaagaatgg ttcttttacc attgattgta acataagtaa
    13441 agggttgtcg ccaactaatt ttaagcttat ataagctact attggcaaaa acactataca
    13501 aatagcaagt tgaataaatg ctgatactgc aactccccac attcctgatg tataagtata
    13561 tataatgata actataactc ctataattac agctatatca taaggaatac caaatgcagt
    13621 agatacagaa ccaaaagaaa tcaattgact acccataata gctagtatag atattggtgt
    13681 tacaagactt tgataagctc ttaaaaatac actatgacca tatctagttt caatataatc
    13741 agcaggtgtt atatcacaag ataagcttct taattttcct gcgaaaaatg catttataaa
    13801 gcaagctaaa gctgcaccta tagtagaata cattccaacc caaagtcctc tttcagctcc
    13861 ccattgaact ccaccaacta agcaccctcc tccaaaagat gtagcagcaa tggttgcagc
    13921 tagtataggc aaaggcagat ttctacctgc gattaggaaa tcggatgctg ttttaacttt
    13981 ttttgaaaaa taaaaaccaa tccatatgta tattgcacac gtaacaatta aaagtaacat
    14041 agtcttttct cctttataaa atattttaat aaaatataca aaatatattt aaaatatatt
    14101 ttatataaaa gtagtacgat atttgtttta ttggttttag attaatatag ctataataat
    14161 ttatgatatt agatggaaat tacgtaaaaa tagaaaattg aaaattctga aatttttgca
    14221 tattgatatt tatctcatag attctttttt gtaattgtaa cacgctttta tttttaaatc
    14281 aatagtacat gtataaaaat aaaaggaat
//
LOCUS       DAYASV010000096        14159 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYASV010000096 DAYASV010000000
VERSION     DAYASV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.097"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            579..1229
                     /locus_tag="ACGI68_002733"
     CDS             579..1229
                     /locus_tag="ACGI68_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFH0139160.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1443..2114
                     /locus_tag="ACGI68_002734"
     CDS             1443..2114
                     /locus_tag="ACGI68_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139161.1"
                     /translation="MAKILIVEDEKRMQSIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            2111..3241
                     /locus_tag="ACGI68_002735"
     CDS             2111..3241
                     /locus_tag="ACGI68_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139162.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKLEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3542..3664
                     /locus_tag="ACGI68_002736"
     CDS             3542..3664
                     /locus_tag="ACGI68_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139163.1"
                     /translation="MKWYTWLTIILIILLSIPLKIKFIKWWDRRQKNDKGGYDR"
     gene            3661..4506
                     /locus_tag="ACGI68_002737"
     CDS             3661..4506
                     /locus_tag="ACGI68_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139164.1"
                     /translation="MIKVGELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSLFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4506..5534
                     /locus_tag="ACGI68_002738"
     CDS             4506..5534
                     /locus_tag="ACGI68_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139165.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDDYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNIGFFEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5537..6550
                     /locus_tag="ACGI68_002739"
     CDS             5537..6550
                     /locus_tag="ACGI68_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139166.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLYYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLF
                     FSR"
     gene            complement(7067..8317)
                     /gene="cme"
                     /locus_tag="ACGI68_002740"
     CDS             complement(7067..8317)
                     /gene="cme"
                     /locus_tag="ACGI68_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HFH0139167.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8403..9149)
                     /locus_tag="ACGI68_002741"
     CDS             complement(8403..9149)
                     /locus_tag="ACGI68_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0139168.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9264..10286)
                     /locus_tag="ACGI68_002742"
     CDS             complement(9264..10286)
                     /locus_tag="ACGI68_002742"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFH0139169.1"
                     /translation="MNKNNCLDLIFEKVNPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTYSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10286..12706)
                     /locus_tag="ACGI68_002743"
     CDS             complement(10286..12706)
                     /locus_tag="ACGI68_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0139170.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSYNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIKMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12792..13712)
                     /locus_tag="ACGI68_002744"
     CDS             complement(12792..13712)
                     /locus_tag="ACGI68_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0139171.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         5019 a         1821 c         2218 g         5101 t
ORIGIN      
        1 ttatattaaa ttaaggacta taatttgaaa ataagctata aaaactcaat attgttttgt
       61 tcaaagataa aaaaagaata ctttaggtga tttttattaa aaattaagag tgataagatt
      121 atatacttta taaatgtaaa tatgacggat aaaaatgata aatataataa tatatatatt
      181 tacattagga gttttctata ctataattat ttaaaactaa atataaatgt tggaattatt
      241 taggacttga aattgtctaa aaataaatat aagtgatgaa gaggtgatgt gatgagatta
      301 gatatggata gtatgttata tgtactaatt tatatgacat tattggtatc tatcattgga
      361 agtatatttt atttttgtaa agacctctta aatcataaaa ttaagtatgt tttaataaga
      421 gacatatttg taaatagaat tacatattct gaagaacggt tgcctaaaca gtatatagtt
      481 tttcagaaat atgatatttg gaggtattgt agtttgtttt aatttgaaat gaatctatta
      541 taaattctaa agtttaaaaa taaaaaggag agaataaaat ggatttcatt tcaagtatat
      601 tgcaaggatt attaagtata ttatttaatt ttacaggtga ttttggaatt tctattgtac
      661 ttataacttt aatagttaaa ttagtactta tgccactatc attaaaacaa agattttctg
      721 taaaaaaaca gcaagaatta gctgaaaaaa tggaacatat aaaagaaaag tataagaatg
      781 atactaaaga attggaaaag caaatacaag taagttctgt agaaagtatg aagagtatgc
      841 tgggatgtct tatagttttg gttcaaatgc cagtagtata tgctttatat catgtatgtt
      901 tagatatgac aaaggaattt acaacaatta taattccatg gattgctaat ttaggtacat
      961 ttgataattt gtttatatta ccttgtatat atacattaat tatgttagct tcaaatttag
     1021 tgaattatat accatattta aaaataaatt ctcaagtaaa gtttaacaaa caaatggctg
     1081 tggtgactac cataacaagt cttatactaa ctgttagaac tccagtagcc ataggtctgt
     1141 actttataac aagtgcaatt tattcattaa ttgaagatat ttgttttaga atatatttta
     1201 atcgaaaaaa tagaatagtg acaaaataat ataattatta ttagaaatat tttagcctgc
     1261 taacatagaa aaagttagta ggctattttt atctatgata tcaattccat taagaatagt
     1321 ctaaaaaata ctatcaaaat gctataaaaa atactatcaa aatactataa aaaaatagtt
     1381 gaaaaataaa taaattaata taaactaaaa taaagcctac aaaaaagagg tgataaatag
     1441 aaatggctaa aatactaatt gtagaagatg aaaaaaggat gcaaagtatt attgtagaat
     1501 atatgcaaaa gggaggatat acttgtataa ctgctgatga tggagtcgag gcacttacaa
     1561 tattaaagag caacaatata gatttaatga tattggatat tatgatgccg tatttagatg
     1621 gattttcagt gtgtagagtt tcacgtgaaa tgactaatat acctattatt atattgacag
     1681 ctaaaggtga agaggaggac aaattaaagg gttatgaata tggcgcagat gattatataa
     1741 caaaaccatt tagccccaaa gtacttttag caaaagttaa tgccttgtta cgtagataca
     1801 ctactgatat tcccaaaaat tcattgagtt taggaaagat ttttattatg gtagcatcaa
     1861 ggcaagtgta tgtagaagat aaattgattg atttaacata taaagaattt gaattattgc
     1921 gtttatttat ggaaaaccct aatcaagttt tttccagaga gaaactttta aattgtatat
     1981 ggggatatga ttttgaagga aacacacgaa ctgtagatac acatataaaa accttacgca
     2041 aaaagcttgg tagtgaagga caccatatag ttacattaat tcgttcagga tataagtttg
     2101 aggttaagga atgaatagtt taagaaagat tatagaaaaa ctgaaaaatg tagatatttc
     2161 aaattggatt aaacaaaatt ttggactata ttcagttaga aaaaaagtgt ttttactttc
     2221 taaattatca ggagcgttaa ttatattatt ttatctggtt gttgaagaat tttcgataaa
     2281 ctctaaatta gaattttgga tttggtttat aatactggtt atatttatta tgagtattga
     2341 tttcttattg ggaaaattta tttcagaacc aatcacatct ataaataaat ctgcaaaaag
     2401 tatgtcccaa cttgattttt ctaatccatg tactgtaaat actaatgatg aatttggaga
     2461 actttctagg agcctgaata caatgtctac aaatctacaa caggcattat cagacctaga
     2521 aagtgcaaat attcaacttg aaaaggatgt aaataaggaa agaatgctat tggaacaaag
     2581 aaaggagttg gtagatacaa tttcacatga aatgaagact ccacttggca ttatacgtgc
     2641 gtatacagag gggcttatgg atgaagttga tgaagaaaaa aagaaaaatt atatgaatgt
     2701 tataatagaa gaaactgata gaatgaacaa tatgattgta tcattattag acttatctgc
     2761 attggaagca ggagtttcta aacttaaccc agagcgtttt gattttatag agtttgttga
     2821 aactgtggca ggtcgtcttt taattgatat accagatgcc aatttttatt ttacatatga
     2881 tttgccagag tatgaagttt ttgtagtggc agataaaatg agaatggaac aagtagtaga
     2941 gaatctagtt attaatgcaa agaagcatgt tgtatataat ggtaatttag atttatcgat
     3001 tacatgtgaa aatggcttgc ttttatttga aatttataat gatggtaggt ttatagagca
     3061 agatgagatt tcaaagattt ggtctaagtt ttatcgtagt gttcaatctc agcgtacagg
     3121 aggttcaggt ctagggcttg caattgtttc acaaatactt acaatgcaag ggttagaata
     3181 tggagtgaat aatcgtgata gaggagtaga gttttatttt atgattccaa ttgatgaata
     3241 atttagtatt aatttataaa ttataagtat ctgatgtaaa atatttttct ttgtatatgt
     3301 aagcatttaa agagaataaa ataaaaaaac ttaatgaaaa aagcttaatg aaaaagtcta
     3361 ataaaaagct taatgaaaag tcttgttttt ctgatgaaaa caagattttt tattaagctt
     3421 tttattattt cacttcgatt tcacaagaga gacactctga tttcaccaaa tagaaataaa
     3481 ataggttaaa taaattaaaa aatagaagcg taattaaata aggttacaag ggggagtact
     3541 tatgaaatgg tatacatggt tgactattat attgataata ttgctttcta ttccgcttaa
     3601 aattaaattt ataaaatggt gggatagacg acaaaaaaat gataaaggag gttatgatag
     3661 atgataaaag taggagagtt aaaattttct tatggaaaag ataaacagat attgcatgga
     3721 cttaattttg aagtaaaaga aggagaaatc tttggattcc ttggacctaa tggttcagga
     3781 aaatccacaa cacagaaaat cttaaatggt gttttaaaag gttacaatgg tcacgtatcc
     3841 ctttttggta aggaagttaa agcatacaca gaatctttat atcaaaagat tggtgtttta
     3901 tttgaatttc cttatttata taccaattta agtgcaattg ataatttaga atatttttcg
     3961 tctttttatc ctaaaaatca aaggagagat atatatgaac tcttagattt gttagagttt
     4021 aagaaagagt ttataaataa gcctgtatca tcctactcaa aaggtatgaa gcaacgtatt
     4081 agtatggcaa gagcattggt aagtaatcca aagttgttat ttttagatga gcctactagt
     4141 ggacttgacc catctggagc tgtactattt cgtaagatta ttgaagaaga acgaaaaaaa
     4201 ggaacaacta tctttttgac tactcataat atgctagatg cagatttaat gtgtaacaat
     4261 gtggctttta ttgcagatgg aaagattatg gtaatagata aacccaaaaa tttaaaaatg
     4321 aaaaatagca ataataaagt tgaagtggaa tttgtatata atggaaatcg agatattgaa
     4381 attgtagata tacaagagtt agaatctgga ataactttta aatatgatga aatcttaagt
     4441 gtacattcaa aagaacctac actagaagaa gtttttatta aatacacagg aaggatgtta
     4501 gtgtaatgta ctctaaattt tttaccttag taaaaaaaga tttttggatg atgatttatg
     4561 gtaaattttt tgtgctgaca cttggctctc tcatacttta ttcgtgttat attaattttg
     4621 gttatgtaaa gtttatggat gaagatagtt atcacaataa tgtatatcta tataatccag
     4681 agaatataag catgagtgca tctccattaa ttaatcaagt ttcttctata ggtgaattaa
     4741 aagcaaaact tgatgaagat acaagtgcag tttgtattga tatgagttcc agtaagccga
     4801 aagtaatttt acatgaaagt attaaaggag ttgacgatta tagagctgac tatgcattgt
     4861 cattattatc atcagaaaaa gaccacaata gcaaaatatc agacaacaat gcaataattc
     4921 aaattaatga taaggataca tctgagataa aacaaagaaa agacatgact tgtgagattt
     4981 tattctttga aattgtagct attggatttt tagggattgc ctctttatta tttaaagaaa
     5041 agcagatggg agtaatgcgt attactggta ttttacctgt aaaaagaagt ctttttatat
     5101 tatcaaagat atttgctttt ttgatatgtg acctatgttt tacagttctt ttagtagtat
     5161 ttaatattgg attttttgaa ggaataaaaa tacttccaca agtattagtt caagctagta
     5221 ttttatcaat tattatgtct ttgattggaa taggatgctc aatgttattg agagatttta
     5281 agcaattttc tcttatatat cttgtggttg ctgtattcat aactacacct atatttctat
     5341 ctgcaaatac atcttttgaa atagattgga ttaaatatca tcccttctat catgtttata
     5401 gtcagcttaa aaatgcgttt ttcatgaatt tttcaaaaga attagtttat tttgtagtgt
     5461 gttctttgac aataatagtt ctatttttac ttgtaaaata tatttttaat aaagagttgg
     5521 ttagggaggg ataatcttga taggattacg ttaccagtta aagagtataa aaaaagataa
     5581 aatgtgtata atttccttct ttttaccagt tgttatggcg attttaataa attttatggg
     5641 aacaattgat atatcttcat taggagagat acgttttggt atagtgaagg atacacttcc
     5701 tgtacaaaca gagcagtggt tgaagaagta tggtatggta agtgtatatg acacaaaaat
     5761 gcatttaata cgtgaaatta aggatattaa aacagacaca attggagttg ttagagatgg
     5821 taaagggata aaaactgtgc tttatggaaa tgagattaat gctataaaaa atgttggaaa
     5881 gacattgcca gagatgttta gactaaaggg tgatttaaaa gatgttgagg ttacaatatt
     5941 gcctaaagat agtattttaa aagagtttca aaatgttttt attgccatga ttttaattac
     6001 tgctatgttt atgggatgca cctttaatgc aatgaatatt atatctgaaa aagaagatgg
     6061 aatatcattt ataaatgaag ttttgccaca gagtagaagt cagtatatta tacaaaaagt
     6121 atttttgggt cttttatgtg ggtgtttatc ttcaatgata actgtattta tatgtttgga
     6181 tatttcaata ttagatatga taatgatgtt gttactcgtt gtattttcag catttatttc
     6241 atcacttata ggtttattta ttggaaagtt gtcagaaggt cttatggtag gaattgttta
     6301 tattaaaata gtaatgattt tatttatagg tactcctttg ttaatatatc ttctaggggt
     6361 taataccagt ggtataggga atttatacta tataatgcct tcagttgcta catttaatgg
     6421 tattatggag attattaatg gtaatgtcat tattatcaaa gatattgtaa ttttggctgt
     6481 acattgtgta gtatggttta tgatatatat tattttagat aaaaaaagaa agttattttt
     6541 tagtagataa ctcagtataa ttataattgg gacaatttaa taggtgattt aatatacttt
     6601 gtatagtgta atttatgtag tgcaatttgt atactttaat ttatgtagtg taatagttag
     6661 ttttatttta taattattca atataattta gtaagaagtt cagtacaatt agagctactt
     6721 agtggaaaac ttaataggta cttaaactat ataaatactt tattttcctc catttgtact
     6781 ataaatttaa tttatgatat aaatggaggt attattatat gatttgataa atttcatcat
     6841 tataacctac aagtaaaggt attattatat aaagttaata aagatagtat caaaatatta
     6901 tttgctataa agagaggggc tcaaaataaa gataaaccta tttttcaatg ttgaaatgat
     6961 aaaatatatt tgatttcata taaaaaattt tcttcataaa ttaaaaagtc cattttaagc
     7021 cccctcattt ttaaccagat aaatcgagtt aaactattta aatatattac tcggattcat
     7081 ttgacttaga tattccttgc ttgtaaaatt gttttgaata ggggatagcc aaagcaaaaa
     7141 ataaaagtat aatccctgct atgattatta aattattaat agaaattaca tctgccattg
     7201 gtccgaaaat catcatacca agaggaataa aaccagtata tataataata ggtaagctaa
     7261 gaactcgacc tagcatttgt ggtgctacat gctcttgcaa catagtcata gctgatgctt
     7321 gaatgattgg aattgaaaaa ttcgtaaaaa acataaatat aaaaaattgc caagcttgtg
     7381 ttacaagacc tagaataatt gaaaaaatag cataggataa tattgctaga aaaaaggtct
     7441 tatttctatt tttaaaacca ccaaaaactc ctataatcaa tccacctaaa accataccta
     7501 aaaatccaat catttcaata atagacaggt atatataacc cttgccaaat gttcgttcaa
     7561 taagaagtcc tataagaaat cctgatggaa cacaaagaag catgaatgct ccataagtaa
     7621 aaagcacttt tctaatgaac ttatctgaaa aacagtactt tatccctgat accatatctg
     7681 caaaaaatga gactttttca gtttgttttg gtatatcatg tttaggaatt ttgatgaata
     7741 tcaataatac aatagcaata attgcagtaa atacatcaat taaaagaact tgatttattt
     7801 tattaccaaa tccaagtatt gcagcagcta caataggaac tataaattgt actatagatt
     7861 gtaggctact atttatacca ttaaaacgca ttaaatgttc ttctggtaca agttgaggaa
     7921 tcattgcgtt tacagcaggt gtttgtacac cacttcctag tgaacgtata acagatatta
     7981 ctgatatggc tattaattca cttgttgtgc catttgatga catcataaat agaattagaa
     8041 ctaatgtaga tatagcaatt gttgaatctg atacaattat gagtaccttt cgattgtagt
     8101 agtctgccca tacaccagca aaaaaagaaa ttatcatttg aggtataaag gagcatacag
     8161 ttaaaactgt tacccataca cctgatgatg tctgaagtgt gacataccat atcattgcca
     8221 tttgtacaag tgatgagcca aataaggaaa tatattgact tgctaaaaaa agaatgactt
     8281 ttttcttcca tgtagaatta gtatttttgt tttccatcct attcctcctt tgatttgtgt
     8341 gttgaatttt tcttgctctt aaggataaca cacaaatcaa agtctggagt tagagtggag
     8401 ggctaggaaa attgtttttt cataatttga agatgagatt tcatggtttt tgcattatgt
     8461 tcagcttctt tcaatgaagt tatcagtttg tcacacactg agataatttc gtcatcatct
     8521 tttggagtgt caaggacagt tttaatatta gtattaggat tagaagataa agcatttaac
     8581 attcgtaaaa tagctgaaag agaatagttt gcacaacgaa gtgcgcttat aattttaagt
     8641 cgttttatat cttcatcagt atatacacga taaccatttt gttttctttt tacttttaac
     8701 aaaccattca tttcccaatt tcttaaagta tctattgtaa cgtttaagtg ttctgcagtt
     8761 tcttttctag ttaaaaagag tttatttgtt tctggagtat ttccatttaa aatctgtgca
     8821 acaatagtaa gtgcttcctc ggcattagat ttttctattt ctaattgttg caagtattga
     8881 tttacaagac taatagcctt atcaaagtcc ctattcgctg atgttcttac aatctcaagg
     8941 gcatcttttc ttaatcccct ttgtaccact tcaattttca aggctgtacg agcaaatcta
     9001 aattgttcta tatgaaaatc attaaagata cgataaccat tggtattacg cttaggttta
     9061 ggtattaagt ttagttcttc atataaacgt actgtattag gatgtacacc aattaaatct
     9121 gctacctctg atgtcgtgta agtgtccatt agtattcaac tccttttaga tatatagtat
     9181 tagtcataat aacaccaaac aaaagtctgg tgttataatg gagggtttgg ttatctaaga
     9241 gtaattaatt tatgaaagag taattaatct attatagaag taagtatata tttttcaata
     9301 aactcccatc tatcacgagg tttaaacact attttagatg gtatggctat tactaagtca
     9361 tattgtggaa tacaacaaat agcatttcct ccagctccca ttgctgaata ccacaaaaca
     9421 ccatcttttt tacatagcca ccacagataa ccgtattcat tactattcat tttaactgat
     9481 tcttcaatcc acgatttaga tataatacgt ttattattcc aaataccatc atttaaatac
     9541 agaaaaccaa atcgcaacat atctctagca ctcaatgtaa gtccccagcc acctatagaa
     9601 taaccctgtc tatcttttgc ccaaccctta agattttttc caaagaaatc ttcaaaacca
     9661 aaatctttca tatcataatc tggtataact ttcataccaa taggtttaaa taagtattca
     9721 ttagcaaatt cacgagcaga ttttccagta gtttttgtta taatagctga aagtaggtgc
     9781 gttccagctg aagaatattt gaaagtacca atttcgcctt tttgaccaag ttgattcaag
     9841 gtatatttta tccaatcttt ttgtttgcaa agtctttcta aagactcagt catattttta
     9901 aaagggtatg gtgcagtcat tgtaagtagg tgacgtatag ttactgcttt tttacatatt
     9961 tcagaaggac tataagtata ttcagggaaa aaatcaacaa ccttttggtc aacatctttt
    10021 atatatcctt tatctattgc aatacctatt aaggctgata taatactttt tgtaactgat
    10081 gcaacatgaa aagtatcatc agcattatat ccattatagt acttttcaaa gacaactttt
    10141 ccttttcttg caataattat tccatttata ttactatatt gtgagattat taaaggttca
    10201 agttgtgaga gtttttctga actcatgcca acacttgaag gatttacttt ttcaaatatt
    10261 aaatctaagc aattgttttt attcattaaa gatacctccc tgttatagat tttggatttt
    10321 cttttagagt ttttggagaa ccttctgcaa ttatataacc tcctttgtca ccaccttctg
    10381 gaccaagctc aacaatccag tcacaaacat ttaatacatc tatatcatgt tcaacaacaa
    10441 ttacagaatt tccattttca accaattcat ctaataattg aatcaactta gaagtatcgt
    10501 atagacttag accagtagtt ggctcatcca atacataaag gatattacca tttcgttttt
    10561 taccaatttc ttttgcaagt ttgattcttt gggattcacc accacttaaa gttggtgtag
    10621 gttggcctaa ttttatatac cccataccta tttgttctaa tacatttaag tgtgaaacaa
    10681 tatttggaat atccttgaag aaatgtacag attcagaaac actcatattt aaaatatcat
    10741 gtatattttt atttttatat ttaatagata aagattcatc attaaatctt tttccctgac
    10801 attcactgca tatatggtct atacttagat tatttcccaa ccagaccttt tcatatccac
    10861 taccactaca tttaggacaa gcccctttgg agttaaatga gaaatgacca gctgtaaatc
    10921 ctcgttctaa cgattctggc tgacctgcaa atagtgtacg tatcttatcc catataccaa
    10981 tataagaagc aggagttgag ttcatatttt tgccaatagg agattgagat atctctgaat
    11041 atccagaaat atgtttagta ccaatcagtc tatctgcaat agtttcaaca actgcataat
    11101 cgtcttcctc acctatttca ctctctgaag aatttatttg attattgata gtatggttat
    11161 taaattggct tctaagaaga ggaagtaggg tgtctgatat aagtgaactc ttgccactac
    11221 ctgattttcc agatattcca accatagcac ccaaaggtaa tgatacagtt acatttttaa
    11281 gattatttgt atttgcattt tgaatagtta tacaaggtat tttatcagta tgtgtaatgt
    11341 ttttattgct tgttctatta ggcattggat attttccaga tagatactgt ccagtgagtg
    11401 atttgtcgga ttgaagtaga ccttctaaat ctccttgata tactaattga ccaccttcaa
    11461 caccagcttt tggaccaata tcaataatat gttctgccat tttaattgtg tttttatcat
    11521 gctcaactac tatgaccgta ttgcctaaat ctttaagttt ctttattgat tttaagatgt
    11581 ctattttttc tgattcatgc aatccagctg ttggttcatc aagcacataa attaatgagt
    11641 ccatctctga atcgagatgt gaatttaaaa atagtctttg catttcccca ccactcaaag
    11701 ttgacatttc tcggtataag gatagatgac ctaatcgtga ttctattaaa tgttgagttt
    11761 tggataaaat atcatttaca agatttgtac caaatgtgtt taaattacct tgatttatta
    11821 agagtttaag gaaatcatca atttctaaaa tcgtcatttt tccaagttct ccaatatgtt
    11881 ttccgtttaa aagtactcta cgtgcttctt caccaatttt aaaaccattg caatctgaac
    11941 aggcagtcat agtataaact ccatttaaat cttcgcccct tttgtaacga ttgtgaaata
    12001 cctttgataa agaggaactt tttttaccat tttcataatg accatagagt acttcatttc
    12061 ttacttcttc aggtagttgt aagaattgta tgtctaaata atcattgaat tttttagaaa
    12121 atactctcaa atatccaggt gtaatctttg ctctatctaa gacttgtttt agggtatcat
    12181 gcttatcaag gactaatttt tcaatattaa tctcaaaata tgcaccatgt ccagagcact
    12241 tcatacacat tccatctgaa gagttgtatg agaaataact tggtcttaat cgttcttcct
    12301 catatccaca attattgcaa attagattat agcttacagg agtatcacac atagtacaca
    12361 ttatttgacc ttccactgaa tacaatagtg taagcatgtt gagaatattg gttcttgtgc
    12421 ctactgttga acgaggattg ctctggcgaa taatattttg ttgaacagct atagtgggag
    12481 ctaaaccttg aatattatca aacttatctt cactatcaat acctgcaagt atacctaaag
    12541 attgcagata ttgtttacgc ccttcctcaa atacaatgtc aaacataagg ctagacttac
    12601 ctgagccact gacacctgtt gcaacaacaa gcttatcttt ggggatagaa acatctatat
    12661 ttttaagatt gtgtatcttt gctccattaa tttttatttc tttcattggt tcacctcatt
    12721 ttatcaattt tatcaaaccg taaaataggg atatattaat ccttaataca agaatagatt
    12781 aggtgaggtt attaaaaagg ttctataggt aaatatatat cgaccaagtg tctattttga
    12841 gggtgattgt gtgggttgtt aagatatact tcaaaaggga aggaggcacg tagcttataa
    12901 ttaccatttg atagccattc attatacata aaatcccacg catcactata ttcagatttt
    12961 gaaatatcaa aatgtcccac aagatatttt ccagaaggta ttatcatatt gccaagattg
    13021 tcattctctt tgacaacaat gttatctgga atggtcatgc aaatactagt ccttaattga
    13081 tgctcctgtg taaattgtgg attatcatga taaatggcta atatttgtgt gttttcaagt
    13141 ccaagtagat tctgctcact tgcatatgtg aataaccgtt ctaaaagttt tgggaaagta
    13201 ctggttagat taccatatga accagtatac ctagtgtaaa tagtattcat attatcaagt
    13261 tctagaattt caatttcacc tttaacctct ctagtattct tacatttagg ttttgatatg
    13321 cttctattgt actgagaaat tttatatggt tctttgcaat tcttgctata attgtttcta
    13381 tattttatag ggcttatttt atagtaattt ttaaatgctc ttgagaaaac tgctgaatca
    13441 gtaaagccaa agtagtgtgc aatatcagta acagtcatgt cagttctatg aataagaaaa
    13501 gcagttgcat tttctagttt tagcctattt acatattgtg ccaaagtttc tttttctatt
    13561 gctttgaaga ttctatgaaa atgaaatttt gaaaaacctg ctatattaga aagttcatca
    13621 agagaaagag gttcatgtaa atgttcttct atatagtctt gcactttgca aatacgattt
    13681 agatattctt ttttactttt agcatttgtc atatatagcc ctccttattg tgtaaaatgt
    13741 gtatcaatta atttatagca atatagtaat aaaattttat gtttttgtaa tttatgataa
    13801 ataagatagc gtttaatttt ttataattga taaaagatat ttataattaa taaaagatat
    13861 aagatgcctt atatttgtct ttgcatataa gtatagtctt cgtaataatt agtataccat
    13921 agtgatttat ttttaattgt gtatttatat aaatacacaa cttatgatat ggacgaatta
    13981 ttatgcaaat aagctgtaat taaataaatt gcaatataaa ccagttgaaa cataagttag
    14041 ggaaatatta tcaaagttat tacttagcat aagagtatag atggaataaa ataagattaa
    14101 aaaattacat actaaattta ataaaatttt tatattaaaa ataatattaa gtttattgt
//
LOCUS       DAYASV010000097        14158 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYASV010000097 DAYASV010000000
VERSION     DAYASV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.098"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            433..1836
                     /locus_tag="ACGI68_002745"
     CDS             433..1836
                     /locus_tag="ACGI68_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0139172.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            complement(2313..3335)
                     /locus_tag="ACGI68_002746"
     CDS             complement(2313..3335)
                     /locus_tag="ACGI68_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0139173.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(3370..4968)
                     /locus_tag="ACGI68_002747"
     CDS             complement(3370..4968)
                     /locus_tag="ACGI68_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HFH0139174.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            complement(5010..6275)
                     /locus_tag="ACGI68_002748"
     CDS             complement(5010..6275)
                     /locus_tag="ACGI68_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139175.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTGIGMSAAVAE
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            complement(6417..7844)
                     /locus_tag="ACGI68_002749"
     CDS             complement(6417..7844)
                     /locus_tag="ACGI68_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HFH0139176.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTEKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SVGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLFASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQIYMGVEE"
     gene            complement(7882..8889)
                     /locus_tag="ACGI68_002750"
     CDS             complement(7882..8889)
                     /locus_tag="ACGI68_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFH0139177.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            complement(9086..10408)
                     /locus_tag="ACGI68_002751"
     CDS             complement(9086..10408)
                     /locus_tag="ACGI68_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139178.1"
                     /translation="MKILYVGPGMVIDKILAVSKKNFPNIEADFLRYDRYIEVPDLLE
                     KYRGRVDAVLFTGKSPFKFFEKRQKDFVLSDYIPRHETTLYRVLLEITYLLKFDISRL
                     SIDTYDKAMMKRLYKEIGVEFKDEHMFFAEQKYLEEDYIKFVLEFHKYNYQNNDVCCC
                     ITGLEEAYHIMKKDNIPVVLALPSEDVMVQSIKNIQMRYIAKKNNENQIIVLAIKLNM
                     PSEYSLMKEDEYAYLSQRIKILDKLYHFNSRIDGVLVEQSRSEFMIFTTKKILELETK
                     KYRDIYLLDMIREVSVINTYIGIGYGKTANEAKFNAYESIKMAQSQKNDAAYVVFENG
                     EIMGPLKPNPTVKEKNSFDERFYQIATETGLSANTVYKVFNKIINSGKTEFTSKELAT
                     ICGVSIRTMDRIILRLCDSGYCEVVSEQLMSKYGRPSRILRFKPFLLF"
     gene            complement(10766..12406)
                     /locus_tag="ACGI68_002752"
     CDS             complement(10766..12406)
                     /locus_tag="ACGI68_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139179.1"
                     /translation="MFDVKIVNGIIVDGTGNSRYKSDVGIVGDKIMAIGDLSQKEAKE
                     TIDATGKFVSPGFIDFHTHSDLSLVYDKYTRSRIHTGVTTDVIANCGIGVAPIREEKK
                     QELIDYLGTRIIGTIPTKLELHWNTMQEYFDYLTENSPAVNVVAYVAQGPVRINEMGF
                     SKEPATLEQLKNMKLEVRKAMEAGCVALSSGLVYLPGAYTKKEEMAELCKELIPYNGY
                     YISHIRDEGDEEMEALDEAIYIAKTAGVPLHISHLKVMGHKNFGTIDEVFKKLDEAEA
                     DGLEVTFDCYPYTAGMTSLGALLPPWAFEGGVENMVKRLEVQENRDRIIKELEEGIPG
                     WQCFYQLAGGWNGVVLASVMTEANKYVEGKTLMEVAKINGENPFDTFFRLLIEEKSKI
                     QVVVHTQGQEDTDKVVCHPKSCIGSDSMDLSTEGLLSLGKPHPRAFGTFGRIFSYYVR
                     EKGMLTFEEAVKKITYLSAKRLGIYKERGLLKENYFADIVVFDPDTIEDKATYSNPKQ
                     YTVGVEYVLVNGKIALAGGKQTDVCAGRVIKNPLSIAK"
     gene            complement(12562..13218)
                     /locus_tag="ACGI68_002753"
     CDS             complement(12562..13218)
                     /locus_tag="ACGI68_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139180.1"
                     /translation="MYDKKMIKIGRISIGLAIIANFIPAIYVGIRFGQMPMLSVIFQI
                     WGLVAATYGVSWIVQPIAYYPTLGAPGSYIGWLAGSVGDIRMPAASMAQKIAGVEAGT
                     HEGEVVGTIGTCCSIFVSASMITIFTIVGYKVIPLLPEFVTNSFNYILPALFGAIYVD
                     IARKDIRAGGCTIAVALAIMYFGEKIGIPGGLLTLFIVLGGILITRVFFVIDNNKTKE
                     "
     gene            complement(13244..13945)
                     /locus_tag="ACGI68_002754"
     CDS             complement(13244..13945)
                     /locus_tag="ACGI68_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFH0139181.1"
                     /translation="MKFQEMINSPGLWIVSSFLVIISVVQAIVFMKEALKEASNLGIE
                     KKGIKAAIRSASVTAIGPSLSPVITLLSLVAVIGAPTTWMRLCDVGAARTELGVISLT
                     SNLSGVEVGSAAFGAEAFSYALWGMALNNLGWLFVVFILGHRMRGIVEKMNMKYNPTW
                     VKKLLAGATVGLFAYLLSNQIKTFEIPKLTPAIISAIVMLVLTTLFKKNQRLQELSLG
                     IAMLIGMFGTQIILS"
BASE COUNT         4811 a         2664 c         1672 g         5011 t
ORIGIN      
        1 tattatattc taatatgcta ctatactaca caacaaatcc tataattata ttctaatata
       61 ctacctacta ctttgcgcta caacaatttc tctatttata tgatttgtaa aatatagtac
      121 aacatttttt atcattttca tttcaactaa atttgtaata ttttattcaa tacttaataa
      181 atacttaatc aatacatgct ttataatact ttactcaata tataatcaat acataattta
      241 taatttgtaa tactttattc aatacataag tagaaatctc tatatacata aattcatttt
      301 ttcatacatc ttacaaattt atttttattt agataacatg tatatttttt ccatgaaaaa
      361 taaataataa aaaatagtaa atatggaaaa tataaaattg attaacaata attaattacc
      421 tgaggtgtga atatgaaaat gctaaacaac ttttttaaga aaatcaacaa tccagctcct
      481 gtctttgcac agacaaaaat ggaaagagat aatcaaacag atgattcact ttctacaaaa
      541 ccaaagacta catttagaga cgttgctgga ttagacgaag taaaagaaga actatttgaa
      601 atagtcgatt ttatgaagtc accacaaaaa tatcaaaaaa tgggtgcaaa aatacctaaa
      661 ggagtattat tttatggtcc tccaggaaca ggtaaaaccc tacttgcatc tgctgtagct
      721 ggtgaaacaa actcatcatt tttcaatgtt actggttcag agttcgttga aaaatatgta
      781 ggtgtgggag caaaaagagt tagaactcta tttgaaaaag ctagaaaaga agctccaagt
      841 ataatattta tagacgaaat agatgctgta ggagcaaaga gacatcttga aagtaacaac
      901 gaaaaagacc aaacactaaa ccaactttta gttgaaatgg atggatttaa taaagattcc
      961 aatgtattaa taataggtgc taccaataga cttgacttat tagatgaagc tttacttaga
     1021 ccaggaagat ttgacagaca cattcatata ggtgcaccaa actatcacac tagatttgaa
     1081 attctaaagg tacatacaga tgacaaacct atagacaaat ctgtaaatct agaactttta
     1141 gctaaaaaaa ctcatggttt caatggggct catctttcaa atatagcaaa tgaagcagct
     1201 atatttgcag ttagagatga cagtgagtgt attacatcag aacattttga caaggcactt
     1261 gaaagagtta ttgcagggct agaatctaaa aactctgcat tggttgaaaa agagaaaaaa
     1321 atagtagcat atcatgaagc tggacatgct cttgtaagtg atatagtagg aatttgtcct
     1381 atacaaaaaa tatctatagt tcctagagga caagcattag gttatgttct tcaattacct
     1441 gatgaagata gatatattta tactaaggat gagttgattg gaaaaataaa aatattactt
     1501 gctggaaagg cttcagaaga attaatattt aatcataaat ctactggtgc aaaggacgac
     1561 ttaaagaaag tcactgaaat agctaaccaa atggtttgtg aatatggtat gagtaactta
     1621 ggatttatga ctattgatgg aaatgataag actttcttat gtgataaggt tcaaaaggaa
     1681 gcaaatcgta tagttgaaat ttgttataaa gagactttag agatgttaaa ggataatctt
     1741 gaagatttac attctgtttc taaattctta tttgaaaaag aaactatgac tcatgaagag
     1801 ttaaaagatt tgataggaaa agaagctgta aactaatata gttttaaaaa tttgaggctg
     1861 tctcaaaata gagataagcc tctttttatt gttaaagtta taaaaataca tttgatttta
     1921 tacaaaaaag tgcctcataa actaaaaatt tcattttgag acacttacat attttaatta
     1981 catattttaa ttttgtctat ttacagaata ttatatctac ataaaactaa actgctgtaa
     2041 acttaactac ctccagtaat gtttgaagtg gtataaaagc tagtagccta gtttctacac
     2101 ttataattta tattttacat aactaaattt agtagatttt tatactcaca atttagaacg
     2161 attgaaaact taaaaatttc ttatcagata tctacaactt aattaggtat ctatcacttg
     2221 attaaatatc tatcacttaa ttaggtatct atcacttgat taaatatctg ccatttaatt
     2281 aaatatctac cacttaatta aatatctacc acttaaagtg tagaacctct ttcaatcaat
     2341 tcaggtttta acatttcaac gactgactct ggattttctt catttaataa ggacttaagt
     2401 atgttaattg ttttttcaat caacaaatct agtggctgtc taatcgtcgt taaattataa
     2461 ctcttccatg aagccatttg aatatcatca tatcctatca ctgataaatc ctctggcacc
     2521 ctcaacttgt actcaaacct tgcagcatcc attacaccaa tagctatcaa gtcacttgtg
     2581 caaaaaattg ctgttggagg atttgataat gctaacattc tcttacctgc ttcatatcca
     2641 gaatcataat catagtttgc agattcttct ttaaggttat gaatcccact ttgtcttaac
     2701 ttagagtaaa atccaatttt cttttccatt tcttcacttg tttctctcgt aaattgaata
     2761 tatcctatat tcttatgacc tttcttaagc agatattcag cagccattcc cccaccttca
     2821 attgggtcta catagactgt acttattttt ataccaggta taaaacgatt aaaaagaatc
     2881 acaggaatat tgttttcttc acaagtctca gccatcacct tattcatggc cgaagctgta
     2941 ataatcacag cttctacttg aaactgaatt acttttgaaa ttatactatc tatactttgt
     3001 tgtctaggaa ctttaaaaac gagacactgt ttacctatct cttgaatttt ttctacaaat
     3061 aaattgataa ttctattgta aaatggacct agactatcac caactatcaa tcctataaca
     3121 ttagtttttc cactaatcat tccccgtgct accaaattag gaacatatcc tatttcattt
     3181 gctacacgaa aaattctttc tcttacttca ggttttacgc cctttccatt tgcactacca
     3241 aatgctcttg aaacagatga ttgagatact cctgccaatc ttgcaacatc ttttgaggta
     3301 gccgatttat tataatgaac tttcttttga ttcatttagt atttctcctt aaatatctag
     3361 tatttcttct taaaattgta tcaaaaataa tataatatat tggataataa aaaaagctaa
     3421 tacaattaat tcttgtttac ctaaagaaaa tggcactata gatactccat ctactttatc
     3481 cattccttga gaaataaggt gatttctcat accaaatgca gtgtattttt taatatcatc
     3541 tgcattatga tttttcttta aaatgacact aaaaatatat ccaaatatca atgttattgt
     3601 agcacctatt gctggattcc acaaccaact cacattatat ttattagaaa taaaaaatcc
     3661 taaaactaac cctacaataa aaccaataga tactccttta tcattagctt tatatgtaaa
     3721 cattgctagt acaaatgcac ctgtagctgg tccagcaaag tatgagatat agttaccaac
     3781 aaggtcaaga attgatttga tgcttcctcc aaatcccata ataataaaac ctgtcataac
     3841 aactcctata actacagtta taatcattgt aacttttaat gatgattctt tgttatcttt
     3901 tttgaaataa aactcataaa tatccttcgt aaaaacagtt gtcatactgt ttaatataga
     3961 gtcgatacta gacattgctg ctgcaaacac acctgcaata ataattccaa tggctcctac
     4021 tggaagttca tttaaaataa aagtaatcat tatttcattt gaagtttcaa atgtccttcc
     4081 cccataaaaa acaaataaaa ttactcctac caatagcata aagtagtaaa ctaaattcat
     4141 aacaaatgca ctaactacaa aagaattttt tgcacatttt aaagatttag atgttaaaac
     4201 tctttgtact tgagattggt caaaacaaaa gtatcttatc cacattacag ttccaccaat
     4261 taaagtagcc caaaaagtat ttgtcactga aatatcccta ctgaaatcaa atgtatttaa
     4321 ttttccactc tgttgtgcaa tttgtaatgt atcaaagaaa cttagattca ttccttttaa
     4381 ggctacaata attactataa atacagcact tattactact atcatttgaa tactatcagt
     4441 ccatataact gccttaatcc cacctaatag agtgtatata attgatacaa gtacaactat
     4501 tgggactaaa gcataaagtg accagccagt aatcatttga ataataagca ctggaatata
     4561 taccatagaa ctagcttgaa tcaatgaatt aataaaaaat tgaataatag ttaatcctct
     4621 tgaataattt cctaaacgaa gtcccatata ttcatatata gaggttattt ttaatttata
     4681 gataactggg gtggtaatgc tcagtgcaaa aaatattgct aaaggtactg taatattaac
     4741 cattactgga tacattccat ctacatatgc ccatccagga ccacctataa agccatttgc
     4801 actaatcata gtagctgcaa ctgataaagc aattggaatc catggcattg acctacctgc
     4861 caagaaataa tcttcttgat ttttattatc ttttcctgaa taataaccaa taactaacat
     4921 taatagcaaa tatcctatta taatacatac atcaattgat gaaatcataa gccacccctt
     4981 tctaagtaac aaaataaatt cctagtattt tattttttat tatttaacgt ctctggtaag
     5041 aataaccata caatagcacc tgctacaaag actgcgattg atatgtaaaa gatagtcatc
     5101 atacctaaag taccagaagc tgtaccagct atagcaggta ttgcaaacgt agatataatc
     5161 cttcctccat taaatgacat accagatgac aacgaacgat atttacttgg gaacaactct
     5221 gtgtagtatg ctccccatgc tccagaatat ccaacagcaa cacctagtaa tactgatatc
     5281 atatagaatg ttgttgtatt atctccacct aattttgtat atataaaaac aaatactgct
     5341 gctaatgcca tacctataaa aggtattttt cttccaaatt tatctgcaat tgaaccccag
     5401 aataaatatc ctactaattg accaattcct tgtaaactat atatagactc agccactgct
     5461 gctgacattc ctattcctgt cactaggtat gtagttgcat tatttgaaaa tgctgaatat
     5521 gctgtaaaat ttgcacctga taaaagaatc gttcccaatc caatccataa gtattttccc
     5581 ttatacatac ttatatagct aatcttttca ccagtttctt ttcccttttt aactaattcc
     5641 tttgtatact ccaaatcatc tggcacaaag aagattgcta agatagctcc aactataggt
     5701 ggtataatcg cagtatacat caatatttgc caatttgcat cacctaaaac gccatatact
     5761 ttcccaccaa aaatcattgc taatgaaaat aaagcagtta atatagcagc taacttacca
     5821 cgatatttac ttggaaacat atctgcaact aaaggcatag ctgttccaaa tacgcctccc
     5881 aacactactc ctgtacacaa acgtgctatt ttccataaca taatatttgt aggagataac
     5941 attggtataa atgttgtcaa tccaatcagt aaagcaacgc taattaaagt cctcttcttc
     6001 ccaatttttg tagaaatcac cccaaaaata aatgtacctg gaattgtacc aaaagacata
     6061 actgcaaata tttcttctgt ttgttttaat gaaaaaccaa atcccccatc tgctaaagca
     6121 taaatcattg ttggtttaat aaaagtaccc accataaaat tgtagcaata aaaaaagtat
     6181 attaaagcaa ttgaaaaaaa tgcaattcct ctttttaggt ttgaatagtc ttttcctaat
     6241 atgatatcat tttctatttc aaccgttgtt ttcatatttt tataattccc ccttttaaat
     6301 ttagtcataa ttttgctaaa agtttaaaca tctatttttc atatacattc cccacttaaa
     6361 ttttataaag caatacttcc ctatatattt caaaactatt tatactctaa acagctttat
     6421 tcttctactc ccatataaat ttgttttaat acatccatac catcaaaagt tatccactcc
     6481 tcctttactt taccttgtag tatatgataa tgattgattc ccattacttc tatatgcttt
     6541 ccagaaggct gtccaaaaaa tcctatacct tcatttattc cacgtaatct ccatcttaca
     6601 gatacatcat aaccttcatt tttttctctt tgattacaag ttactctttc cacttcataa
     6661 cttccatttg gaattgatgc aaataaactt acaatcattc cttgaatctc atcatagcca
     6721 tttaaatctt tatcacatat aaaatgtact actgcattat catgatagta ttttttaact
     6781 tcatttatat atttataatt ataaatacgg cttgtcattt caagcatcat ttcgccaaca
     6841 gaatcatctt ttgcttgata cttagttggc ataaattgac catccataga ttcacagata
     6901 ccataagttg attgtaaact aagcacttta gattcacttg cctttgccat actctttgct
     6961 acttcctgag ggtttaatcc taattgagta acaatccata aattatctct aaccaaccat
     7021 tcttcatgga ttctattgtt agttgtggca caatcaatta cagttctaaa gtttattttt
     7081 ctacctgttg ctggtccaaa attactatca cctaagttag ttgctgtaga caaaacacgg
     7141 tgagaagata aaaaaccatc agcaccaaag ttagaccata ttacattttg accaattaat
     7201 cttctatcag gaaacgcatg taatgtttga agagtattgg aaataacatc tttaatacca
     7261 actaaatttg aagagccaca atgcattgta acattattgt gatatgtatc ataaataaca
     7321 cctatacctt tttcttccca gatattatgt gtaatcttca taatataatc tacaaaatct
     7381 ctatacttag aatcaaatcc aggtatttcc tgttcccttt ttgtctgttt tgaaaaatca
     7441 ttgtaatcca ttactccaac aggcgttact tctaatgaat ctccatagta aatttttgta
     7501 gatgacatat tatcttcccc cttaaatata acttttttat ttttttcagt aagagtattt
     7561 gtatttaatt tatctgtatc cactgtatct atgttaactt tatttatgtc ggatttattt
     7621 gtgtttgttt tatctatatt ccctttatct atatttcctt tatctatatt tcttttatat
     7681 gtgttcgttt tattctcatt tgtttgattt tcatttatat tactaaaatt agtttgattc
     7741 attttagctt tgttttcttt aaccgtacat gattcaagcc cacttgtaga aacttctttt
     7801 tcttcctctt tacttgttgc attatttttg ttggtagatg ccactttata tccccctttg
     7861 ttttagaata cgtatgcata actaagcatt aaaaatactt ttagttctct caagaatatc
     7921 aagcccttgt tgtttcaacc aataaggaat atcaattaac acccagtttt cttgaagttt
     7981 atcgccctta cgataataaa catcaacaac ttgcatatct gctttaacct ctccacctgg
     8041 taatcctaaa aaacctccac atggtacatt agtcagatta ggccatccaa agaaacaagc
     8101 aaaattacct tctgcaaatc tacatacatg cccattaaaa gtctttcctt ttaaattatt
     8161 tctaaatggt agctggtgtt gctcttgata tctaggtata gtataagaag caccaattcc
     8221 acctggacca taccaaatca tatctttagc ccaagatttt tcaagtattt ctggtgggca
     8281 tcccatagca ccactttcat ttagttcaga taaatcatct accattttat ttactaatgc
     8341 taaagttttt actccttcaa cttcatctgc atcttcaaaa agtaatccat tatggtcaat
     8401 tggccctgga tatacaaaat atgaacctgt ttgtggtggc aatggattaa ctcctgcttg
     8461 aatcataaac ccaattatat ctacaaataa tccagtttta gcaattttac catcttgaac
     8521 acagttaaat tctgcatatc ttaaactaat cattttacga gtatgtttaa tgccaagcca
     8581 atcgttatca aaaagtccca taaaatgccc catactcatt acccattcac tattgtcaat
     8641 ctcatttgtt cctgcaatga aaatatcttg tcgtctttgc atattactca atgatttttt
     8701 taaaggcttc caaaatacat ctgctacatt ttcagtgcct tcttgttcac gaaatggata
     8761 cacacctttc cagttataat ctgatttcat atgttgcttt aagacatttt caacctcatc
     8821 cccagtagca ttttctaatg cttcaaaata atttcttaca actttttttg aatcctgata
     8881 ttttcccata attatctcct cccagattta tttaagttat aaattaactt tctcatttca
     8941 tgcatacgat ttccttttta atgatttttt agctgtttta agtttatttt atgaatacat
     9001 atgcattttt ctaaatatta acatatatct aaaaaattgt aaagaaaatg ttatcataaa
     9061 aatttaattt ttgacttata tctatttaaa acaacagaaa aggtttaaaa cgtaatattc
     9121 tactgggtct accatacttg ctcataagct gttcactcac tacctcacaa taaccagaat
     9181 cacacaatct aagtataatc ctgtccatag ttctaatact tacaccacat atagtagcta
     9241 attcctttga tgtaaattca gtttttccac tatttattat tttattgaat actttatata
     9301 cagtattagc acttagccct gtctctgtag caatttgata aaaacgttca tcaaaactat
     9361 ttttctcttt gacagtagga tttggcttta gtggtcccat aatttctcca ttttcaaaaa
     9421 ctacatatgc tgcatcattt ttttgacttt gagccatctt aatactctca taagcgttaa
     9481 atttagcttc atttgctgtt tttccataac caattcctat ataagtgttt ataactgaaa
     9541 cttcacgaat catatccaac aaataaatat ctctgtattt ctttgtttct aactcaagta
     9601 tttttttagt tgtaaaaatc ataaattcac ttctactctg ctctaccaaa acaccatcta
     9661 tacggctatt gaaatggtat agtttatcaa gaatcttaat cctttgtgaa agataagcgt
     9721 actcatcttc cttcattagt gaatattctg aaggcatgtt cagtttaata gcaagaacaa
     9781 taatctgatt ctcattgttt ttctttgcaa tataacgcat ctgaatgttt tttatacttt
     9841 gcaccattac gtcctcagaa ggcaaggcta aaactactgg aatattatct tttttcatta
     9901 tatgataggc ctcttcaaga cctgtaatac aacaacatac atcattattt tgataattat
     9961 atttatgaaa ttcaagcaca aactttatat agtcctcttc taaatacttt tgctctgcaa
    10021 aaaacatatg ttcatcctta aattcaactc caatttcttt atacaatctc ttcatcatag
    10081 ccttatcata agtatctata ctaagtctac taatatcgaa tttaagcaaa tatgtaattt
    10141 caagcaacac tctgtacaat gttgtttcat gtctaggaat ataatcagac aatacaaaat
    10201 ctttttgtct tttttcaaaa aatttaaatg gcgacttacc tgtaaaaaga actgcatcca
    10261 ctctacctct atatttttcc aataaatcag gtacttcaat atatctatca tatctaagaa
    10321 aatctgcttc aatattagga aaattctttt tacttactgc tagtatttta tcgattacca
    10381 tacctggtcc tacataaagt attttcattt cgtctctcct ttcctaatga tactattatc
    10441 tcttaaaaca ttttctaaac ctaccatgac taaagccaca ggactcctga ttcatagaat
    10501 tttgaataat caattatacc taatataaaa agttacttta agttttttaa ttacgactat
    10561 ctgttgaaac atttttgtat ttaaattttt gtatttaaat cttattcatt aatattaatt
    10621 acattttaca attaatatat tgttttttct acttaaagtt aaattcttta gaaaacacaa
    10681 gtagagtaat ctttattaat ttaacaaaaa tagccttcct taatattcaa agaaagacta
    10741 tttttataaa agttcttaat gtattctatt ttgctatgct aagaggattt ttaattacac
    10801 gtccagcaca tacatcagtt tgctttcctc ctgccaatgc aattttacca ttcaccaaca
    10861 catattccac tcccactgta tactgtttag gattactata agtcgcttta tcttctattg
    10921 tatctgggtc aaataccaca atgtcagcaa aataattttc tttaagtaga cccctttctt
    10981 tataaatgcc taagcgtttt gctgaaagat aagttatctt tttaactgct tcctcaaaag
    11041 tcaacatacc tttttctcta acatagtaag agaaaattcg tccaaaagta ccaaatgcac
    11101 gaggatgagg ctttccaaga gaaagtaaac cttctgtaga aaggtccata ctatcagaac
    11161 ctatacatga ctttggatgg caaacaacct tatctgtatc ctcttgacct tgtgtatgaa
    11221 caactacttg aattttactt ttttcttcaa tcaaaagcct aaagaacgta tcaaatggat
    11281 tttcaccatt gattttggct acctccatta aagttttacc ctctacatat ttatttgctt
    11341 cagtcattac agaggctagt acaacaccgt tccacccacc tgctaattgg tagaaacatt
    11401 gccatccagg aatcccttcc tccaactctt ttataattct gtctctattt tcttgtactt
    11461 ctaatcgttt taccatattt tctacaccgc cttcaaaagc ccaaggaggt aaaagtgcac
    11521 ccaaagatgt cattccagct gtatatgggt aacaatcaaa agttacttcc aaaccatctg
    11581 cctcagcttc atctagcttt ttaaatacct catcaatagt tccaaaattc ttatgaccca
    11641 taacttttaa atgagaaata tgtagaggaa ctcctgcagt cttagctata taaattgctt
    11701 catcaagagc ttccatttct tcatcacctt catctctaat atgagatata tagtatccat
    11761 tgtatggtat caattcctta cataattcag ccatttcttc tttctttgtg tatgctcctg
    11821 gtaaataaac taatcctgat gaaagagcta cacatccagc ttccatagct tttctaactt
    11881 ccaacttcat atttttaagt tgctctaaag tagctggttc ctttgaaaat cccatttcat
    11941 tgatacgtac aggtccttgt gctacataag ctacaacatt gactgctgga gaattttcag
    12001 ttaagtaatc aaagtattct tgcattgtat tccaatgaag ctctaatttt gttggaattg
    12061 taccaattat cctagttcca agataatcaa ttaactcttg cttcttctct tctcttatcg
    12121 gagcaactcc tataccacag tttgcaataa catctgttgt tacacctgta tggatacggc
    12181 ttcttgtgta tttatcatac acaagagata aatcagaatg agtatggaaa tctataaaac
    12241 caggtgatac aaattttcca gttgcgtcta tagtctcttt agcctctttt tgacttaaat
    12301 ctccaatagc catgattttg tctcctacaa tccctacatc acttttatat cttgaatttc
    12361 ctgtaccatc aacgattatt ccattgacaa ttttaacatc aaacatgctt tttctccttt
    12421 caagacttat ttatattcat atttcttaat aaacaatcct attctttttt ctcaacaaat
    12481 aattctagtc tttctcctta ataaacaatc ctattctttt tctcaacaaa taattctagt
    12541 ctttctcctt aataagcaat cctattcttt tgttttattg ttatcaatta caaagaagac
    12601 ccttgtgata agtatcccac ctaacacaat aaacaatgtt aataatccac ctggtatacc
    12661 aattttttca ccaaagtaca taattgctaa cgctacagca atcgtacatc caccagctcg
    12721 aatatcctta cgtgcaatat caacataaat tgctccaaat aatgctggaa gtatataatt
    12781 aaatgaatta gtaacaaatt caggtaacaa aggaattact ttatagccta caatggtaaa
    12841 aatggtaatc atagaagctg atacaaagat tgaacaacat gtaccaattg ttccaacaac
    12901 ttcaccctca tgagttcctg cttctacacc tgcaattttc tgtgccattg atgctgcagg
    12961 catacgtata tctccaacag aacctgcaag ccaacctata taagaacctg gagctcctag
    13021 tgtaggataa taagctatag gttgaacaat ccaactaacc ccataagtcg cagcgactaa
    13081 cccccaaatt tgaaatataa cagaaagcat tggcatctga ccaaagcgaa tacctacata
    13141 gatagcggga ataaagtttg caataattgc aagacctata gaaattctcc ctatttttat
    13201 catcttttta tcatacataa ttatcacctc ttatccgttt taatcacgat aatataatct
    13261 gtgtaccaaa cataccaatt aacatggcaa tacctaagga taattcttgt aatcgttgat
    13321 tctttttaaa taaagttgtc aatactagca taactatagc tgaaataatt gctggtgtaa
    13381 gttttggtat ttcaaatgtt tttatttgat ttgaaagaag gtaagcaaat aatccaactg
    13441 tagcacctgc aagtagtttt ttaacccaag taggattata cttcatgttc atcttttcaa
    13501 caattccacg cattctatga cccaaaataa atacaacaaa tagccaacct aagttattta
    13561 aagccatccc ccatagtgca tacgaaaaag cttctgctcc aaatgcagca ctacctactt
    13621 ctaccccaga taaattggat gtaagagata ttactccaag ctctgttcta gctgctccga
    13681 catcacagag cctcatccaa gtcgtaggag cgccaatcac agctaccagt gataataatg
    13741 taataacagg agatagagaa ggtccaatgg ctgtaacact agctgagcga attgctgctt
    13801 taattccttt cttttcaata ccaagatttg aagcctcttt tagtgcctct ttcataaata
    13861 cgattgcttg aacaactgaa ataattacta aaaaactaga aacaatccaa agacctgggc
    13921 tattaatcat ttcttgaaac ttcatacttt attcctcctt aaaataaatt tttcagcatt
    13981 ttattgtgag ctttctttct gtaatcttgt tatgttttta tattgtttta atttatttta
    14041 tctaagatat agtatcttta ataggagatt atctgtctta aagtctcact aaaaacatta
    14101 atacttaaat attttattca taattagtaa atatcagaaa attcacatat tatttttt
//
LOCUS       DAYASV010000098        14127 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYASV010000098 DAYASV010000000
VERSION     DAYASV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.099"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            538..1737
                     /gene="hadA"
                     /locus_tag="ACGI68_002755"
     CDS             538..1737
                     /gene="hadA"
                     /locus_tag="ACGI68_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HFH0139182.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            1776..2576
                     /gene="hadI"
                     /locus_tag="ACGI68_002756"
     CDS             1776..2576
                     /gene="hadI"
                     /locus_tag="ACGI68_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HFH0139183.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            2591..3817
                     /gene="hadB"
                     /locus_tag="ACGI68_002757"
     CDS             2591..3817
                     /gene="hadB"
                     /locus_tag="ACGI68_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HFH0139184.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            3817..4944
                     /gene="hadC"
                     /locus_tag="ACGI68_002758"
     CDS             3817..4944
                     /gene="hadC"
                     /locus_tag="ACGI68_002758"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HFH0139185.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            4992..6125
                     /gene="acdB"
                     /locus_tag="ACGI68_002759"
     CDS             4992..6125
                     /gene="acdB"
                     /locus_tag="ACGI68_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative isocaproyl-CoA dehydrogenase AcdB"
                     /protein_id="HFH0139186.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            6158..6943
                     /gene="etfB"
                     /locus_tag="ACGI68_002760"
     CDS             6158..6943
                     /gene="etfB"
                     /locus_tag="ACGI68_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HFH0139187.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            6962..7999
                     /locus_tag="ACGI68_002761"
     CDS             6962..7999
                     /locus_tag="ACGI68_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0139188.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            8482..10113
                     /locus_tag="ACGI68_002762"
     CDS             8482..10113
                     /locus_tag="ACGI68_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0139189.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10248..11174)
                     /gene="fba"
                     /locus_tag="ACGI68_002763"
     CDS             complement(10248..11174)
                     /gene="fba"
                     /locus_tag="ACGI68_002763"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0030388 - fructose 1,6-bisphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HFH0139190.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            11504..11833
                     /locus_tag="ACGI68_002764"
     CDS             11504..11833
                     /locus_tag="ACGI68_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HFH0139191.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESIDLAFTVFEKEKY
                     YENGEHF"
     gene            11833..12555
                     /locus_tag="ACGI68_002765"
     CDS             11833..12555
                     /locus_tag="ACGI68_002765"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFH0139192.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            12582..13943
                     /gene="rlmD"
                     /locus_tag="ACGI68_002766"
     CDS             12582..13943
                     /gene="rlmD"
                     /locus_tag="ACGI68_002766"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFH0139193.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVLL
                     RRK"
BASE COUNT         5560 a         1638 c         2722 g         4207 t
ORIGIN      
        1 ttgatattta gtaatttttt atttaatgta ataattactc taaaatttat caaagaaatt
       61 gttatttttt agattaaata ttattaaaaa attatctaag tttgaaatta gttaactaac
      121 ttgctgatta attacaatga taacatgtgt ttttgagttt agcaatgaaa actttttttg
      181 aaatgtcaag ctttttcata aaaaaatata taaaaaaaac ttttataagc atagaaagaa
      241 tagcaaaatg caatggatag tggcaagatg ccacgatatg aaaaagatta ttcaattgca
      301 tattgttaaa aaacattcaa atcaagcgat attttgatta aatataaagt tggcacgata
      361 aatgcttaac aatataggtg taagacaata aaacaataaa aaataatttt aataattttt
      421 aagttatttt agataagatt aggaactttg acatcagaca gttaatatca gaaaaaagta
      481 ttttatgagt gatttaatcg aaattttata attgatacta ttaaggagga aaacgttatg
      541 cttttagaag gagttaaagt agtagaactt tcaagtttca tcgcagcacc atgttgtgca
      601 aaaatgttag gtgactgggg tgcagaggtt attaagattg aacctataga aggtgatgga
      661 ataagagtta tgggtggaac atttaaatct ccagcatcag atgatgaaaa ccctatgttt
      721 gaattagaaa atggaaataa aaagggtgta agtattaatg taaaatcaaa agaaggagta
      781 gaaatattac ataaattatt atcagaagca gacatatttg taactaatgt tagagttcaa
      841 gcattagaaa aaatgggtat agcttatgac caaataaaag ataagtatcc aggattaata
      901 ttctctcaaa tattaggata tggtgaaaaa ggacctttaa aagataaacc aggatttgac
      961 tatactgcat acttcgcaag aggaggagtt agccaatctg ttatggaaaa aggaacatct
     1021 ccagcaaata cagcagcagg atttggtgac cactatgcag gtctagcact agcagcagga
     1081 agtttagcag cattacataa aaaagctcaa actggtaaag gtgagagagt aacagtaagt
     1141 cttttccata cagctatata tggaatggga acaatgataa caacagcaca atacggaaat
     1201 gaaatgcctt tatcaagaga aaatccaaac agcccattaa tgactacata taaatgtaaa
     1261 gatggaagat ggattcaatt agctttaata caatacaaca agtggttagg caaattctgt
     1321 aaggttataa atagagaata tatattagaa gatgatagat ataataacat agattcaatg
     1381 gttaatcatg ttgaagattt agttaagata gttggagaag ctatgttaga aaaaacatta
     1441 gacgagtggt cagctttatt agaagaagca gacttaccat ttgaaaaaat tcaaagctgt
     1501 gaagatttat tagatgacga acaagcttgg gcaaatgact tcttatttaa gaaaacatac
     1561 gatagcggaa atacaggtgt cttagttaat actccagtta tgtttagaaa tgaaggaatt
     1621 aaagaatata caccagcacc aaaagtaggt caacatactg tagaagtatt aaaatcttta
     1681 ggctacgatg aagagaaaat aaataacttt aaagatagta aagttgtaag atattaatta
     1741 gtaattctag attttttaaa aggagaatgc taaagatgta cacaatggga ttagatatag
     1801 gttcaactgc atcaaaggga gtaatcttaa agaatgggga agatattgta gcttctgaaa
     1861 caatatcctc tggtactggg actactggac catcaagagt tttagaaaaa ttatatggca
     1921 agacaggtct tgcaagagaa gatattaaaa aagttgtagt tacaggatat ggaagaatga
     1981 actattcaga tgctgataag caaataagtg aattaagctg tcatgctaga ggggtaaatt
     2041 tcataattcc agagacaaga accattattg acataggtgg tcaagatgca aaggtattaa
     2101 aattagataa taatggaaga ctattaaact ttcttatgaa tgacaaatgt gctgcaggta
     2161 caggaagatt tttagatgta atggcaaaaa taatagaggt tgatgtatct gaactcggaa
     2221 gtatatctat gaattctcaa aatgaagtat caataagcag tacatgtaca gtatttgcag
     2281 agtctgaggt tatatcacat ttatctgaaa atgcaaaaat tgaagatata gtggcaggta
     2341 ttcatacttc agtagcaaag agagtttcta gcctagtaaa aagaatagga gtacaaagaa
     2401 atgtagttat ggttggtgga gttgctagaa atagtggtat tgtaagagct atggcaagag
     2461 aaatcaacac agaaattatt gtacctgata tacctcaatt aactggtgct ttaggagcag
     2521 cgttatatgc ttttgatgaa gcaaaagaat cacaaaaaga agtgaaaaat atataaaaag
     2581 agggatgaaa atgtctgaaa aaaaagaagc tagagtagta attaatgatt tattagctga
     2641 acaatatgca aatgcattta aagctaaaga agaaggaaga cctgtaggtt ggtcaacatc
     2701 agtatttcct caagagttag cagaagtatt tgacttaaac gtattatatc cagaaaacca
     2761 agcagctgga gtagcagcta aaaaaggttc tttagaatta tgtgaaatag ctgaatctaa
     2821 aggatattct attgacctat gtgcatatgc aagaacaaat tttggtcttt tagaaaatgg
     2881 tggatgtgaa gctttggata tgccagctcc agatttccta ctttgctgta acaatatatg
     2941 taaccaagtt ataaaatggt atgaaaatat ttcaagagaa ttagatatac ctttaataat
     3001 gattgataca actttcaata atgaagacga agttactcaa tcaagaatag attatattaa
     3061 agctcaattt gaagaagcta taaaacaact agaaattata tcaggaaaga aatttgaccc
     3121 taagaagttt gaagaagtaa tgaaaatatc agctgaaaac ggaagactat ggaagtattc
     3181 tatgagttta ccagcagatt cttctccttc tccaatgaat ggatttgact tatttactta
     3241 catggctgta atagtttgtg ctagaggtaa aaaagaaact acagaagcat ttaagttact
     3301 tatagaagaa ttagaggaca acatgaaaac tggtaaatct tctttcagag gggaagaaaa
     3361 atacagaata atgatggaag gtataccttg ttggccatat ataggataca agatgaaaac
     3421 attagctaaa tttggagtta acatgacagg tagtgtttac ccacatgctt gggcattaca
     3481 atatgaagtt aatgatttag atggaatggc agtagcatat agtactatgt ttaacaatgt
     3541 aaacctagac cgtatgacaa aatatagagt tgattcttta gtagagggta aatgtgatgg
     3601 agcattctat catatgaaca gaagctgtaa acttatgagt ttaatacaat atgaaatgca
     3661 aagaagagca gctgaagaaa ctggattacc atatgctgga tttgatggtg accaagcaga
     3721 ccctagagct ttcactaatg ctcaatttga aacaagaatt caaggtttag ttgaagtaat
     3781 ggaagaaaga aaaaaactta atagaggtga gatataatgg aagctatttt atctaaaatg
     3841 aaagaagtag ttgaaaatcc aaatgcggct gtaaaaaaat ataaaagtga aactggtaaa
     3901 aaagctatag gttgtttccc agtttattgc ccagaagaaa ttatacatgc agctggaatg
     3961 cttccagttg gtatatgggg agggcaaaca gaattagatt tagctaaaca atatttccct
     4021 gcatttgcat gttcaataat gcaatcatgt ttagaatatg gattaaaagg tgcttatgat
     4081 gaattatctg gagttattat accaggtatg tgtgatacac taatttgttt aggacaaaac
     4141 tggaaatcag cagtacctca tataaaatat atatcattag tacacccaca aaatagaaaa
     4201 cttgaagctg gtgtaaaata cttaatcagt gagtacaaag gcgtaaaaag agaacttgaa
     4261 gaaatttgtg gatatgaaat agaagaagca aaaattcatg aaagtataga agtttacaat
     4321 gaacatagaa aaactatgag agactttgtt gaagtagctt ataaacattc taatactata
     4381 aaaccatcaa taagaagctt agtaattaag agtgggttct ttatgagaaa agaagaacat
     4441 actgagctag tgaaagattt aatagcaaaa ttaaatgcta tgccagaaga agtctgttct
     4501 ggaaagaaag ttttattaac aggtatatta gctgattcta aagatatatt agacatttta
     4561 gaagacaaca atatatcagt tgtagctgac gacttagcac aagaaacaag acaattcaga
     4621 acagatgtac cagcaggtga tgatgcgtta gaaagattag caagacaatg gtcaaacata
     4681 gaaggatgtt cattagctta tgaccctaag aaaaaacgtg ggtcacttat agtagatgaa
     4741 gttaaaaaga aagatataga tggtgttatc ttctgtatga tgaaattctg tgacccagaa
     4801 gaatacgatt atcctttagt tagaaaagat atagaagata gtggaatacc tactttatat
     4861 gttgaaatcg accaacaaac tcagaataat gaacaagcca gaactcgtat tcaaactttt
     4921 gctgagatga tgagtttagc gtaagttcat taaaataaat ttactataaa atatgaaaat
     4981 tggaggaagc tatgttatat aacaaagaac aagaactttt aagaaaagca gtaagagatt
     5041 ttgttagtaa agaattagat actttaccag cagaaatgga taaaacaggt gtaatgccta
     5101 aggaattaat caaaaaatta gctgatgcaa aatttataag cagtaatatc ccagaagaat
     5161 atggtggtgg gggagcagga tatgtttctt acgctatagt aatggaagaa atagctagaa
     5221 gatgtgcttc aacagctact tttgttacag ctggttcttc tcttgcttca ttaccaatac
     5281 tttacaatgg tactgaagag caaaaacaaa aatacttaaa aggtatagca acaggtgaat
     5341 taataggagc atttggttta actgagccag gagctggttc tgatgcaggt ggacaacaaa
     5401 caacagcaga attagttgga gaccactata tattaaatgg tagaaaaact ttcataacta
     5461 atggaccatt ctgtgatgta gctatagtaa tagcagttac agatagaagt aaaggactta
     5521 gaggaacatc agcgtttata gtagaaagta aatgggatgg tttctcaaca ggagctcatg
     5581 aagataagat gggtataaga ggaactgaaa cttctgactt aatatttgaa aacgttaaag
     5641 ttccaaaaga aaacttactt ggaaaagaag gtcaaggatt taagatagca atgggtactc
     5701 tagaagttgg tagaataggt gttgctgcct tagctctagg aatagctcaa ggtgctttag
     5761 atgaagctgt taaatataca aaacaaagag ttcaatttgg taagcctata gctaaattcc
     5821 aaaatactca atttactata gctgacatgg aaacaaaagt ttgtgcagct agaggattag
     5881 tttatgatgc agcacaaaag agagatgcag gaatgagagt tgctcaagaa tctgctatgg
     5941 ctaaatacta tgcatcagaa attgcaaatg aagttgctta taaagcatta caacttcacg
     6001 gtggatatgg atttataaaa gattatgaaa tcgaaagaat gtacagagat gctagaatcg
     6061 tatcaatata cgaaggaaca tcagaagttc aaaaaatggt aatttcatca aacgtattaa
     6121 aataataaat aatctaatga aattggaggt atagactttg aaaatattag tttgcgtaaa
     6181 acaagttcca gatactaacg aagtaagaat aaataaagaa acaggtactc ttataagaga
     6241 tggagttcca agtatattaa acccagatga tgcaaatgca ttagaggaag ctttaaaaat
     6301 taaagatgag catgatgatg taactataac agttataaca atgggacctc ctcaagcaga
     6361 ctttatgcta agagagtgtt tagctatggg tgctgatgat gcaatattat taagtgatag
     6421 agcatttgga ggagctgata cttgggctac atcaaatact attgcagcgg gaatatctaa
     6481 agtaggtgac tatgacataa tatttgcagg aagacaagca atagatggag atactgcaca
     6541 agttggacca caaattgcag agaaattaga cattcctcaa gttacatatg tacaagattt
     6601 caaaatagaa ggaaaagata taatagttca aagacaatta gaagatggat acgaattaat
     6661 aaaagtaagt acacctgtac ttttaacagc agtaaaagag ttaaatacac ctagatacat
     6721 gtctgttgat aaaatagtaa aagcatataa acatgatgta aaagtttgga caattgatga
     6781 tttagatgta aataaagaag aagttggttt aaaagcatca ccaactaaag tatttagatc
     6841 atttactcct gaaccaagag gaaaaggaga aatcttagaa ggtaaagctg atgaaatagc
     6901 tagtaaaata ataatcggtc taaaacaaaa gcacattata taagttggag gagtacaaac
     6961 tatgaatgat ataaaagatt taagttctta taaaaacgta tggatatttg cagaacaaag
     7021 agaaggaaaa atagctccag tagttataga attattagga gaaggaagaa aattagctaa
     7081 agaagtagat gcagaacttt gtgcaatatt attaggaaaa gatgttgatg gattagctaa
     7141 agaattaatc acttttggag ctgacaaagt ttatgttgca gatgatgctc ttttagaaaa
     7201 atatacaact gatgcatata caaaagtaat aaaagatgca atagatgaaa taaaaccaga
     7261 aataatgctt ttcggagcaa ctcatatagg tagagactta gcacctagaa tagcttcaag
     7321 agttggaact ggattaacag ctgactgtac taagttagaa atagacccag aagataagaa
     7381 aataaaacaa actcgtccag catttggtgg aaacataatg gctacaatca tttgtccaaa
     7441 ccatagacct caaatgtcta cagttagacc tggtgttatg gataaagctg aaaaagacga
     7501 aacaagaact ggtgaagtta tagcattaga ctacaaaata actcaagatg atataagaac
     7561 tactgtttta gaaacagtta aaactaagaa agatttagta tctcttacag atgcaaatgt
     7621 tatagtatca ggtggtctag gattaggtgg accagaagga tttgaaatgc ttaagaaatt
     7681 agctgacaaa ttaggtggag tagttggttc ttctcgtgcg gctgttgatg ctggatggat
     7741 agaccattct caccaagtag gtcaaacagg aactactgtt aaaccaaacc tatatatagc
     7801 ttgtggtata tcaggagcaa tacaacattt agcaggtatg caatcatcag atttcataat
     7861 tgctataaac aaaaacccag cagctccaat tttagaaatc gctgactatg gagtagttgg
     7921 agacttacat gaaatagttc caatgcttat agaaaaatta gatagcgttg atgatttatt
     7981 agaagctata aaagcttaat agatacaaac tatataaata ttactgattg aataagtctt
     8041 attagttagt aacataatta ttaacccttt gataagttat tgctagtgtg tatttgtcag
     8101 ttattaaaaa gtctgtttga ataaaatttt ataatacaag ataatctgga taatccttat
     8161 atgtatattg cttacaaatt gtgagttata ttctgtaagg attatctttg tttttttaaa
     8221 taaatcttaa atttaattaa attgtattaa atagacaaat aattactaaa tatgtgataa
     8281 aatacaatta aaagcaaaat gaaattttta acaaactata atcattaaaa ttatatctta
     8341 gttacgtttt aatgaaataa actacttgaa aagacgaata ttataaggag ttgattatat
     8401 gtgctgatgg tatttattgt aagcaaacaa taaattttgt atgtagttca ataaattact
     8461 taatgaaagg tgcgaatatt aatggataat aatttaatag ggcttataga acatgtagaa
     8521 aatccagcca ttttatgtaa agaatcaggc gaaataatat actgtaatca tctaatagat
     8581 agtattttta gttttctaga tataaaaaag cctagaaata taaatgaatt agattcaaat
     8641 tttgacaaaa cagaaatact gacagatagt aaaaagaaaa tagctttcag agagttaagg
     8701 atgactgcac atatatacaa tatgaaagat aataataatg aaaataatat agtttattta
     8761 tttgagaagt ctctaatatc tgataaagta attgaagata taatagagca tatagatgaa
     8821 gttgttgttg tgtttaacaa agatggggta attgaaaaga tgaacactgt cagtgatgag
     8881 atattacctt ttaaaagaac tgaggtactt ggtagaaata taacagacct tgtgagacaa
     8941 ggattagttg aagaaccaat aatattaaac atgcttaaag tgaagaaaaa gatttataga
     9001 aatatcgttt acccagatgg gaagcttatt gcatatacgg cagttccaag atgggattct
     9061 aagggaaagc ttacaggagg agtactaaca gggagagata tttcaagggt tattaagctt
     9121 gaatctcaga taaaatatag tgatatatca gaagacacag agtatataag tcaaagtaag
     9181 attatggaca atataaaaaa agttgttaag agagctgcag cctcagattc ttctatattt
     9241 ataaatggag aatcaggtgt aggaaaagaa ataatagcaa gaacaatata taagtatagt
     9301 tcaaggagag acaaaccttt tatagctata aactgtggag ctataccaaa tgaattatta
     9361 gagtcagagt tttttggata tgaagaaggt tcttttactg gagctaagaa aaaaggtaaa
     9421 aagggacttt ttgaagaggc gaatggtgga actattttct tggatgagat aggtgagtta
     9481 ccaatgcaga tgcaaaaaaa attacttagg gttatacaag aaaatactat tactagaata
     9541 ggtggtagta agcctataaa aatagatgtt agatatataa gtgctactaa tatttctcat
     9601 gaagacctta gaaataacct taaatttaga caagatttgt actatagatt aagtgttatt
     9661 ccagtaaaaa tacctcctct tagagaaaga aaagaggata tagtaccatt agtaaattat
     9721 ttcttaaaac tttacaatga aaagtacaat agagaagtag aagtttcgcc taaagttatt
     9781 gagttattag aagaatattc ttggccagga aacataagag aattaaaaaa catcattgaa
     9841 agatttgtag tattatcagc taaaaatgta attggagaag atgaatttaa catgctgata
     9901 aatttagata tgattgataa tgaaacagat gatttatcac caatagtggt aaatggaatt
     9961 atgaatctta atgatgcata taaaatagtg gaccaaataa tgataagtaa ggctataaat
    10021 aagtatggtt caataacaaa ggctgctgaa gttataggta tatatccttc taccatccat
    10081 agaaagataa agagtggaca tattcacgtt tagctgtcag tttaaaaaga caatataatt
    10141 aaaatattat aagatacata atttatacaa cttaaaagag atggcttaaa aataaattta
    10201 attactttaa gtcatctctt ttcttttata aatatttaaa agttatatta gtttatagag
    10261 ttagcagaac ctaaaacatc atctatcttg ctttcaacta cagcttgtat agcatctcta
    10321 ccagcaccaa tgtattttct tgggtcaaat tcttttggat tttcagctaa gaattttctg
    10381 atagcagcag tcatagctag tcttaagtca gtatccatgt ttattttaca aacagccata
    10441 gaagatgctt ttcttaacat atctacaggt acacctttag caccagctat atttccacca
    10501 aattcattac atgtagcaac agcgttttgg tcaacagcag atgcaccatg aagaactatt
    10561 gggaaacctg gtaatttgct ttggatttct tctagtatat cgaatcttaa tttagcttct
    10621 cctttgaatt tgaaagcacc atgagaagtt ccaatagcta ttgctaatga atcaacacct
    10681 gttctttcaa cgaactcaac tgcctcagct ggttgagtat acttgtgaac atcagaagta
    10741 acatcatctt ctgttccagc tagaacacca agttctgctt caacaacaac acctttagag
    10801 tgtgcatatt caacagcttc tttagttatt ctaacatttt cttcaaagtc aaaatgagaa
    10861 ccatctatca taactgaaga gaaaccagca tcaatacaag ttttaatagc atccatattt
    10921 ggaccatggt ctaagtgaag agctacatct acacctattt catcagaagc agccttaacc
    10981 atttcaacta atgtatgagg tccagcatat ttaacagctg acatagaagc ttgtatcata
    11041 acataagaat ttttagcttt agcagctttt aaaacacctt gtaattgttc taagtcactt
    11101 atgttaaatg cacctatagc aaatccacct tcgtaagctt ttttaaacat ttcttttgta
    11161 gtaattaatg ccattttaaa ctcctcctat taacttttaa aatttaccca tcacttatat
    11221 tataacgtta aatcaaaaaa agtatatagt aaattgtaat aactataaat tacaagtata
    11281 atgttaaata gaagacaata ggattaaata aaataataaa gaatataaac aatttaggaa
    11341 aactagctat ataatgctaa ctataagggt aggaactacc ttattagttt ggagatactg
    11401 ataacaatag ttgccttgac caagaagtta ccacttcaaa cgttatcgaa aggaattaag
    11461 tggatgataa ttcactaaaa atttaaaata aaggagataa actatgccac agataaaaat
    11521 aagaggaatt aatgaaaatg atatatgtaa aataagtgaa aaaatgataa atgatttagt
    11581 tgaagctgta aaatgtccaa gagactactt tgaaatagaa tgtataaagt cagttgcaat
    11641 aagagatggt aaaatagcag atgtatatcc ttttgttgaa gtagcttggt ttgatagagg
    11701 acaagaagta caagatatag tagcaagaat aataactgat agtataagaa ataatttaga
    11761 tgtagaaagt atagatttag catttacagt atttgaaaaa gaaaaatatt atgagaatgg
    11821 agagcatttt taatggctaa aaagcaacgt atagataaaa ttctttcaaa tttaggttat
    11881 ggaagtagaa gtgaaataaa gaagtattgt aaacaagggt ctgtagtagt aaatggaagt
    11941 gaagtttcaa atccaggaac tcaagtagac actgaaaatg atgagatatt atttaatgga
    12001 gaagaagtta tatatagaga atatatatat ttgatgatga ataaacctga tggatatata
    12061 tcagcaacta cagataaata tgacccaaca gtattagatt taattgattt atcatactta
    12121 gcttttgaac catttcctgt tggaaggtta gataaagaca cagagggatt attagtttta
    12181 actaatgatg gaaagctttc tcatagagtt ttatctccta agaagcatgt gcctaagact
    12241 tattatgcaa aaattgatgg agtagttact gaagaagatg tagaagcttt tttagaaggt
    12301 gtagtgcttg atgacggata taaaactatg ccatcacagt taaatatatt aaaaagtgat
    12361 gatgaatcag aaatagagct tacaatacat gaaggtaagt ttcaccaagt aaaaagaatg
    12421 tttgaaagcg taggcaaaaa agtagtatac ttaaaaagat tgtctatggg caatttaaaa
    12481 ttggatgaaa gtttagagtt aggtgagtat agagagttaa cagatgaaga agtaaaattg
    12541 attgaagaaa gataataaag atactatagt aaattggagg aatgtctgtg aagaaaaaag
    12601 atgttataga atttgaagta gataaaatgg aatttggtgg tacatcttta agccaagttg
    12661 gagatagagt tatccatatg aagggtggaa taagtggtca aaaggtgaga gctggagtta
    12721 aaaaagttag aagtaagaaa gctgaagtta agatgataga attattagag aactcaccac
    12781 ttgaaactga agaaacttgc aagcacttta gagagtgtgg aggatgtaca ctattgtctg
    12841 tgccatatga aaaacaatta gaaataaaag aaaaacaagt tatggattta tttttaaagc
    12901 aagatttatt tggatttcag tttttaggga tagaaggaag tccagaaaac aaatattata
    12961 gaaataagat ggaatatact tttggagatg aagtaaaaaa tggacctttg acattaggtc
    13021 ttcataaaaa aggaaagcat atagatatac aaactgttga ggagtgtatg ttggttgatg
    13081 aagacttctc aaagatatta gtttctagtg tagagttttt caatgaaaaa aagcttcctt
    13141 attatagaac aatgaatcac aaaggatatt tgagacatct tgtagttaga aaagggattc
    13201 atactaatga gattatggtt aatatagtaa cttcttctca agaagatttt gatatgtatg
    13261 agtttaaaga tatgctttta gggattgatt taaaagctga gttagtaggt gtacttcata
    13321 ctattaatga tggtttagca gatgcagtcc aatgtgatga gttaagagta ttgtatggaa
    13381 gagattatat acaagaagaa attttaggtc ttaagtttaa gatttcacca ttttcgtttt
    13441 tccaaaccaa tactaaggga gcagaggttc tttatagtat tgctagggat tttattggag
    13501 attacaatga taaagttgta tttgacttgt atagtggtac tgggtctatt ggacaggtta
    13561 tggctggtgc tgctaagaaa gtgtatggta ttgagatagt tgaggaagct gttgttgctg
    13621 ctaatgagaa tgctaagttg aatggactta ctaattgtga gtttattgct ggggatgtgg
    13681 ctaaggttgt taaggattta aaggacaagc ctgatttgat tattgttgac cctcctagac
    13741 caggcattca taaggatgct attagggata tttgtggatt tggtgctaag gagattgttt
    13801 atatttcttg taatcctaag tctttggttg ttgatttggt tgattttaag gggtatggtt
    13861 atgagattaa gatggttaag tgtatggata tgttcccaaa tacgccacat tgtgagactg
    13921 tagtgttgct acgtcgaaaa taatgctaga aatcttgagc tttagacagt ttcatagaca
    13981 tataatattt agattagagg gtaataaaat taggaataaa gaattgaaaa ggtgttttga
    14041 tgtgtctgta gttatatata tggagtgtgg gaatacgaaa taaaaaaagt tttaattata
    14101 gaaatagata ttaaatttat aaattta
//
LOCUS       DAYASV010000099        14103 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYASV010000099 DAYASV010000000
VERSION     DAYASV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.100"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(20..832)
                     /locus_tag="ACGI68_002767"
     CDS             complement(20..832)
                     /locus_tag="ACGI68_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HFH0139194.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            1254..2405
                     /locus_tag="ACGI68_002768"
     CDS             1254..2405
                     /locus_tag="ACGI68_002768"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139195.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVISDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            2523..3161
                     /locus_tag="ACGI68_002769"
     CDS             2523..3161
                     /locus_tag="ACGI68_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HFH0139196.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            3162..4184
                     /locus_tag="ACGI68_002770"
     CDS             3162..4184
                     /locus_tag="ACGI68_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFH0139197.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            4174..4794
                     /locus_tag="ACGI68_002771"
     CDS             4174..4794
                     /locus_tag="ACGI68_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139198.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDIKMNDYMENCNM
                     MAGLALDKIGIVCLDYINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(4918..5604)
                     /locus_tag="ACGI68_002772"
     CDS             complement(4918..5604)
                     /locus_tag="ACGI68_002772"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFH0139199.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQNKRLENSKIKGYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDVLGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(5618..6163)
                     /locus_tag="ACGI68_002773"
     CDS             complement(5618..6163)
                     /locus_tag="ACGI68_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139200.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            6391..7638
                     /locus_tag="ACGI68_002774"
     CDS             6391..7638
                     /locus_tag="ACGI68_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFH0139201.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLTFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            7725..9719
                     /locus_tag="ACGI68_002775"
     CDS             7725..9719
                     /locus_tag="ACGI68_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0139202.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIKNFIENIVKLQYKMLQLKNVEIKIDCEHNLICYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDVLKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            9719..11041
                     /locus_tag="ACGI68_002776"
     CDS             9719..11041
                     /locus_tag="ACGI68_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFH0139203.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNFEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            11320..12606
                     /gene="grdG"
                     /locus_tag="ACGI68_002777"
     CDS             11320..12606
                     /gene="grdG"
                     /locus_tag="ACGI68_002777"
                     /EC_number="1.21.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     alpha"
                     /protein_id="HFH0139204.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            12692..13999
                     /gene="grdF"
                     /locus_tag="ACGI68_002778"
     CDS             12692..13999
                     /gene="grdF"
                     /locus_tag="ACGI68_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_082007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:13739..13741,aa:Sec)
                     /transl_table=11
                     /product="sarcosine reductase complex component B subunit
                     beta"
                     /protein_id="HFH0139205.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
BASE COUNT         5616 a         1495 c         2400 g         4592 t
ORIGIN      
        1 aaaattataa aatttttatt tatttaattt tttcaacaac attagtcaat attgaatcta
       61 aatcatcact aatttctgta actccattat gagtaatatc tataatttct ttagtcatag
      121 gaagttctcc taaaagctct aagtctaaat catctaaaaa cttttcagta gattctcctt
      181 caaataactt gattttttta gaacaatcag gacattggat ataactcata ttttcaataa
      241 ctccaaggac atttatgttc atttttttag ccatattaac agcttttgat acaatcatag
      301 ataccaaatc ttgaggtact gaaaccataa ctattccact tataggaata gattgcataa
      361 ctgttaaggc aacatctcca gtaccaggag gcatatctat taataaatag tctaattctt
      421 cccaaagaac atcagtataa aattgcttta caacccctcc aagtaaaggt cctctccaaa
      481 caacaggttc attttcatca tctatcataa ggtttataga cattactttt atgttatttg
      541 agttttctac agggtaaata taagagccat cagaataagc ttttacattt tttacaccca
      601 taagtctagg aatacttggt ccagttatat ctgagtctaa tataccaacc ttatatccca
      661 ttttgtttag cttattagca agtaaagcag ttacagttga ttttccaact ccacccttcc
      721 cactcataac accaattatt tttccaaatt tattgttagg gttattttca atagaacaat
      781 ttgattgact attacaattt ccctttgatg gacatgaatt acaatctgcc atatctatta
      841 cctccaaact ttaatattta ttagattatt tgtaacctac atatactatt ttagtacaat
      901 tgaaccataa agtcattatt taaaagcaac ttagaagatt ttctattgat ttattcatag
      961 actatagcaa aaagaataca ataaaaaaat tataacattc ttgaaaaagt gattatttca
     1021 atttagttat agaacttagt catataaata tatagaaaat attttaatat gttatacata
     1081 acgggttatt ttataagttt aactataaat taggtttata ttttataaaa atttgctaaa
     1141 aaaaacaatt aaaaattgta aagaaaaaag atataattag aactgtagta ataaatgttt
     1201 tattatagat aatagagttt taagtatctt aaataatatt tggaggaata tgaatggaat
     1261 taagaccaag aagtgcaaaa ttagaagaat ttgataaagt aatagaatta ataaactatg
     1321 tatttagaat atcaagaaat cataaaccaa ctatgatgga agaatttcct cttttactaa
     1381 gtaaaaataa tatagaaaat atgattataa tatcagaaga tgataaagtg atttctgatg
     1441 taaactatct aattcaggat gtatctattc aaggaaacag attgaaggtt gcagctatag
     1501 gtggagtatg tactcatcca gattatgaaa agagaggcta ttcatctaag atattggata
     1561 aagtagaaga aaaaatgttt tatgatggtg tagatattgt aattatatca ggaacaagaa
     1621 gtctatacag tagaagaaat tgtagtttgg taaagagttt ttataaatat acaataaaac
     1681 ctgaagatgt aaaaatagca tatgaaatag ttgaatttga tgaaactaat tttgaaaaag
     1741 ataatgattt agataaaatg attgaactat ataatcaaaa tagtactaga tttataagaa
     1801 cgagagatga gtttcaaaaa ctattgcatg cagcaactat tgcctgggga ccaattggtt
     1861 acaagaaggt atttataaag gaaaataata atataatagg atatttgata ataagaacaa
     1921 taaaaaaaga agattctact gtaggagaag ttgctgaaat agggttaaac tctgtaaatg
     1981 ttgaaaatat attgaaatat gttgcaaata aatttggatt agaatattta aattataaag
     2041 tacatgtgaa aaatcttaaa gaccagttga aatgtaatgg gacaaaatct ttggattatc
     2101 aacaaggaac tatgaaaata ataaatttta ctaagttgtg tgatagttta agaagttatt
     2161 ttagtcaata tgtagatttt gaattactaa aatacatgga atttaaacaa gttgaaaata
     2221 aatatattat taaatacaaa gaagaagaac ttgtaataga aaatttggat aagcttaata
     2281 aactgttttt tgaaaaaaat gaggaacagt acaatgaatt taaacatcta aaaaatatat
     2341 atgaatttgc tacaaaggca tttcctgtag attttccatg gactgcaaat ttaaattatc
     2401 aatagtttta gatgatatta aataaaattg attgatacct agagttagtc cttgttaaat
     2461 aagagaaatt attggatatt gaaaaagcaa ttttgtaagt aattagaaag gatgagtatt
     2521 atatggacat acttgatgat atagcagaat gtatattaaa tatgggtgta gataatatag
     2581 agaatttagt aaaaattgct atagataaaa atattgatgt ggaagatatt tatgaatatg
     2641 gtttaaacaa aggtatgata ggagctttag ataagtttga gaataaggaa tactatcttt
     2701 cagaagttat agtatgcaca gatgcattaa ataaaggtat taatcttttg aagggaactg
     2761 gaaaagtaaa gaaaaaatca aagggagtaa ttctgatgtc tgtagtagaa ggagatactc
     2821 atgagatagg aaaaaatatt gtaaaagtaa tggtagaagc cactggatat aaggttattg
     2881 acttaggtgt aaatagaaaa agtgaagata taatagaaga agctataaaa aacaatgtag
     2941 atataatagg cttatcttca atgatgacaa ctacaatgga aaatatgaaa tcggttatag
     3001 acgaacttaa tatgattgag atagataaaa gaccaaaagt aataattggt ggaggacctg
     3061 taagtatgga gtttgcagaa gaaataggag cagatggata cagttcaaat gctccaaaag
     3121 cagttaaatt aataaataaa cttataggag gtgaagttta aatgttttca gatatgactt
     3181 tacttcagta tataaatagt gaagatagag gtttttttct gcctgatatg ggtacaaatg
     3241 gattgttctt aataaataaa aaggcatatg aagtatatga tagtgtagat ttgcaattag
     3301 aattagcaaa gactatggac aagtattttg aatcagattt tatatactca ttctgtgatg
     3361 ggattacatt ttgtgaaact ctgggtttag agacattaag accagattat gattttccaa
     3421 gtgtattgac acatactata actgacagag ataaattaag aaaattagat gttccagacc
     3481 cttatacgag tggacgtatg ccactcaata tagagagctt atcagttata tcaaaaagta
     3541 ttaaaaaacc actatatgaa tcgatacaag gtccatttac acttgcaggt caattagctg
     3601 gagctactca acttttaaga tgtataataa ctgataaaaa atttgtagaa gaattattgg
     3661 aatttacaac tgaattggta agaagatatg ctgtagctgc aaataaagca ggagcaaaat
     3721 atatttcaat agcagagcca acatcagtaa ccctaagtaa agatagattt gatgaattta
     3781 tagtaaaaaa tttaaataaa atctacaatg aacttgattg ttggaaaggt atgcatatat
     3841 gtggagacac cagagaatta ttagataata tgctaagttg taatatagat gcagtaagtt
     3901 tagaccaaat tttagactat gaagaaattg ctccaatgat tcctagagat atagttttga
     3961 ttggtaattt agacccaata aaacttcttg gacgttctaa gccagataag attagaagag
     4021 agacgctaaa attgttaaag aagatgagaa gatatgataa ctttttatgt gattttggct
     4081 gtaactgctt aaataccact ccagtagaaa atttacaagc agctataaag gcaggaagaa
     4141 ttagttataa agaattagat aggattgata ttgatgaatt ataatagtaa taaaaataaa
     4201 tatcttttaa aggatataaa tttagatgat atttttatag ataagaatat tgtgctaaag
     4261 tttttggggt atggaaatag aaaagctcca gatacaatta gtaaaatcgt tgaaagagaa
     4321 ataagaaata taaatgatat tttagatatc aagatatata taagtgaagt tgatattaat
     4381 aaaacaccta aagaatgtaa aaaagcattt gctgtactat atacaatagg ggataaaatt
     4441 gatattaaga tgaatgacta tatggaaaat tgcaatatga tggcagggct agcattggat
     4501 aaaataggta tagtatgttt agattatatt aatgagaaaa taaaaatgta tttaaaagga
     4561 aagtatgata gtctcaaaat atcgcatgaa atatatccag gagataaaga ttttgaagta
     4621 gagagacaaa aggatattta taactatgta aaaaataaat ataatagtat agaaatagaa
     4681 attaatgatt atcatcaact atcccccata aaaagtgttg caatgcttat tttaatgggg
     4741 gatgaagaaa atttagaatc aagatgtagt aagtgtccta gaaaatgctt ttagttaatt
     4801 ttcaagacta agatttgttt tgtgtataaa ttgcaataat ttttttggtc attttatgta
     4861 taaactaaaa aaattattgc aaagtaatat tctatttaat ttgaacagac ttacaaacta
     4921 tagtagtttc tttaattcaa taagtgtatt tatttcataa gttggtatat gattagtata
     4981 atttatactg ttattggaat ttaaccaaca agtatctatt cctgaattta ttccacctaa
     5041 gacatcagag gttaagctat ccccaattat taaggcagaa gatttatcat gacaattaaa
     5101 ctttttgaga gcgtatttaa atatttcagg atttggtttt gatattttca actcttcaga
     5161 tacaaccatt ccatcaatat atccttttat ttttgaattt tcaagtcttt tattttgaac
     5221 tgcagaaatt ccatttgtta ttataacaat agtatacttt tttttaagat attgtaatat
     5281 atcgattgca cctggaatta aaaatgtaca ttcacctaaa aaatttaagt acattttgct
     5341 aagagtttca gaatcaacat ccaaagatat tttatttgca aaaagttcaa atctaagtaa
     5401 tttaagttca ttcaaagtta taatattttt ttcaaggtcg agccaaagct tatcacttat
     5461 atttctatat gactctatat aattctcaaa attaaattct aaatcaaact cagatagaag
     5521 ttttttaaaa gcatgtgatt cacttttctt aaaatcaaat aaagtatcat ctgcatcaaa
     5581 aaatataaat ttatatgtct tcatggtttt tactccttta caagatgact gcaaaaatta
     5641 ctttactttc actatttgag tcattttgcc atttatgttt tgttcctaaa ggaactttta
     5701 cactatctcc tttggacaaa gtaaattcat catcatccat aaaaagttta acttctccct
     5761 ccaaaacata tgcaatttca tctccattat gagtaatcct atcaacacaa gaagaagtgt
     5821 ttggagctaa atccataatt gcaaattcta aattaccact agagtctgga attaaaatct
     5881 catatataac ttctttgctt ccaggtaaaa taactttttt tctattatca tgtcttacta
     5941 tcaagttttt tttatctact tcagatacaa aaaatgtaaa taaaggaaca tttagagaag
     6001 aggctataga ttttaatgaa tttacagatg gattagcaag acctcgttct atttggctca
     6061 ataatgatgg tgtaacatca gctagttttg ctaaatctcg aatacttaag ttttgttttt
     6121 ttcgaacttc agctattttt tcccctaaat ttaaatcatt catataaata tcctctcttt
     6181 ttaatttaaa aaatattaac ttaaaataat caagtaaaat gattatgtag tatgaaatta
     6241 ttataacata tagaattagg ttttgaaaaa actatgttca atataattaa ataaattaaa
     6301 atataattga caaagtggat acagaatact taaaatatat ttaaataaaa ttaagcaaaa
     6361 gatagttaat ttatgatagg aggggatatt atgcaaataa cgacttttgg agctatattt
     6421 ggtttattaa tagcaattat tttaattata aaaaaatttc aagctgtata tagtttaatg
     6481 ttaggagcat ttattggagg attagttgga ggagcaaata taacacaaac tgtagatttt
     6541 atggcaaatg gagctatgaa tatatcacca tcaattctta gagctttggc atcaggtgta
     6601 ttagctggaa gtttaataaa aacaggggca gtagataaaa tatcagaaca gattgtaaaa
     6661 atatttggtg aaaagagggc tttattttcg attgccattt caactatggt acttgctgga
     6721 gtaggtgtaa atttggatgt atctataata actgtagcac ctataggatt atatataggt
     6781 agaaaattaa attattctaa attgtcaata ctattagcta tgttgggagg gggtaaagca
     6841 ggtaatataa tatcaccaaa tccaaatact atagcagtag cagataattt ttctgtcaat
     6901 ctctctagtg ttatgatggc aaatataatt ccagcaatta ttggagttgt tattacagtt
     6961 attttagcaa gtatattaat aaataaagga aataaagtac aatcatatga aattttagag
     7021 caaagagaag atttaccaag tttatttaaa tcattatgtg gtccaattat agctatattt
     7081 ctattgtttt taggaaatgt atctcctata gttatagacc caatgatagc ccttccaata
     7141 ggaggaatcg taactctaat tgtcacagga aacttgaaca attcaagaga atatttaact
     7201 tttggattat ctaagatgca aggggtatgc atattattac ttggaacagg gactatcgca
     7261 ggaattatac agatgtctga attacaacag agtacaatag gtgctttaca atttttaaat
     7321 atgccacaat ttttattggc accagtttcg ggtatactta tgtcattagc aacagcatca
     7381 tcaacagctg gtgctactat agcatcatct acttttcatg atgctataat aaatggtggg
     7441 ctatcaccga tttcaggtgc atcaatagta aatgctgggt ctagtgtatt cgagcaatta
     7501 ccacatggct cactatttca tactagtgca ggtagtataa acatggatat aggtgaaaga
     7561 tttaagttaa ttccatatga agcattgata ggaatagtaa tgactataat ttcaacatct
     7621 attcaattag ttttataata aatataaata tttaatgaaa aaagtaggat tttgttatta
     7681 aatattgaac ttattatagt aaaaaagaag attggagttt taaagtgaat aaaaaaaaga
     7741 ttgttattat agggattatt tattcatttt tagtagtatt ttcacttaca aatatgtatg
     7801 taaatatgga gtataatcta aatgtatttg aatatattaa gaagtcactt ccatttacag
     7861 aagaagaaaa aaagtggtta gaaaaacaca agaatttgat ttacagctct gaccaaagtt
     7921 cacctccttt gagatataaa ggtaaggaag atggacagta taagggaatt attgttgatt
     7981 taataaattc tctgtcaatt caaattggta gagattttta ttttaaacca aataattggt
     8041 ggaaagaatc tttcgtaaat tcaatagatg atagtataaa gttttttgac ttgattccat
     8101 caaaagaaag ggcaaataaa tttatattta ctgacccaat atacactttg agtgcaaata
     8161 tattgaaaga taaaaagtcc caagatataa atagttacat ggatttaaag gggaaaacag
     8221 tagcaattcc agaaggtgat tattctataa attttttaaa gcaaaaaatt caagatataa
     8281 acattttatt gactccagat ataaagactg gagtcaacca tttaatgtct ggaaaagtag
     8341 acgcagttgt tggtgatgaa ccagtattgc gatactatat aaataattat ggactttcaa
     8401 ataaatacag tgttttaagt aatcctatat atactaaaaa agcagttctt gctgtaccta
     8461 aacaatatga agagctggtt agtattttaa ataaaggcat ttttaaactt cagaaaaatg
     8521 gagtgtataa agatttaaag aaaaaatggt attctacgta taatgaagtt gatgatatat
     8581 tgtatgagcg aggaattgtg cctagtattt atctttttat aggtataatt ttaatatcaa
     8641 tttatgtttt ttatagctat acatatttgt taaagataga aatcaaaagg aaaacagaac
     8701 aggtaataga aaataaaaaa actttagagg caacttttaa tagtattaca gatataataa
     8761 tgcttgtaga tgaaaataac aatatagtag aatcgaacaa agtactatat gactttatgg
     8821 gtgaaatgag ttataagata gcagatttga ttagcatgat aaaaggtgtt attgaaaata
     8881 ccttctctga aaatactaat aaaactagtg aaatagaaat acacaataaa attttaaaga
     8941 taaacacttt tcctgtagag tataaaaaaa ataatactga gtatatagta gttcttatta
     9001 aagatataac taatgataaa atagtagagg cgaaattact tagagaaaac aaaatgatat
     9061 ctatagggca attagcttct ggggtagcac atgagataag aaatcctttg ggtataataa
     9121 gaaataattg ctatctctta aaagataacg ttacaatgga agaagtaaat gattgtgtta
     9181 aatcaataga aagtaatgta gatagggcaa gtaatataat aaccaacttg ttaaatttcg
     9241 caagaatatc agatgataat ttagaacata taaatataaa aaattttatt gaaaatatag
     9301 tgaaacttca atataaaatg ttacagttaa agaatgttga aataaaaatt gactgtgaac
     9361 ataatttaat atgctatata aatggagaat cgttaaaaca tgtatttata aatttaattt
     9421 ctaattcaat agatgcaata catcaagatg gaaaaattat tatatactgt tatgaaaaaa
     9481 atcattgttt atttatagat tttaaagata atggtgaagg aataaaagaa gatgtcctaa
     9541 aggacatatt taatcccttc tatacaacaa aaccaattgg tgagggaact ggtttaggtt
     9601 tatatattac ttataatgag attaaaaaga ataatggaga tataagtgtt gaaagtaaat
     9661 tgggggttgg aacttgtttt catataaaaa ttccattaaa taaagaggtg actatataat
     9721 gaaaatacta ttagttgatg atgagctaga atatggagtg gtaatgaaaa aaattctaca
     9781 aaagaagggc tatcttgtag atatagcact aagtggtgaa gaagctataa atataattaa
     9841 aaaagataaa aattatgatt tggttttatc tgatgttatg atgaaaaata tggatggagt
     9901 acagctatta gatagaatta aatctattaa tagagatata gaggttatat tagttacagg
     9961 atatggaagt attgaaaatg cagttgatgc tatgaaaaga ggagctttat cttattttat
    10021 aaaaagtaat cccattgaaa atttattaga agaagttgaa aaggtaaagg caagtaaaat
    10081 atctgtttct gtacaaaaaa ataattttga atttactttg gaatctaaaa atagagattt
    10141 taataatatc ataaaaatag caaaaaaagc tgcttgtaaa gatgttaata tattaatctt
    10201 gggggaatct ggtgtaggaa aagatatatt agctagatat atacatagca taagtccaag
    10261 aaagaatgag atatttgtac ctgtaaattg ttgttctttt tcagaaaatc tattggagtc
    10321 tgagctgttt ggtcatgaaa aaggttcttt tacaggagca gtagatagta gaaaaggtag
    10381 atttgaactt tcgaataaag gaactctatt tttggatgaa ataggagata tacctctaaa
    10441 tgttcaggta aaacttttga ggacacttga ggacaaatct atagagagga taggaagtaa
    10501 taaatctatt aaagttgact ttaggttaat ttgtgctatg aataaagaac caaaagtgga
    10561 aatatcaaat ggaaatataa gagaagattt tttttataga ataagtacaa taacaataac
    10621 tataccacca ctaagaaaga gaagagaaga cttaactact cttatagaat ttttcttaaa
    10681 taagtaccaa attgaacatg ataaaaagat acactcgatt gataaggaag taaaggattt
    10741 tttattaaat tacaattatc caggaaatat aagagaatta aaaaatataa taaatagact
    10801 tgtagtttta agtgaagaag gtaatttatc aaaagacaat ttaaatttaa tatcaaataa
    10861 tgtatacata gatgataaaa taagtattag acctttaaga gaaataagaa aagagtttga
    10921 atgtgagtat attgaaaaag ttttatcttt gtgtggaaat aatatttcaa atacagctaa
    10981 aaaattggag attagtagaa gacagttaac caataaaata tcagagtata atattaagtg
    11041 agaaaaaaac ttctcattaa gtgagaaaaa aatttctcaa actgtattaa aagataaaca
    11101 gaaaaaatct gtttatcttt ttttattata attttaaaaa ttaatattta caataattat
    11161 tgctaaattc aatattggtg taaattaaaa atgaaaattt ctatgacatt aatacataga
    11221 atatatattt taaataattg gcatagaaaa tgctttatat attaataaac gtaataaata
    11281 cttttaattg cgattaaata tattaagggg gattttcata tgaagctaga gctagggaat
    11341 atatttataa aaaatgttga atttggagaa aagacagaag taaaagatgg agtactatat
    11401 attagtagcg aagaaataga aaagatagta ttagaagatg aaaggattgt aagtgtaaat
    11461 gttgaacttg caagacctgg cgaatcaata agaatagctc cagttaaaga tgtaatagag
    11521 cctagagtta aaattggaga tgaaagcaaa atatttcctg gaattataaa taaagtgaag
    11581 actgtaggaa gtggaagaac ccatgtactt ttaggagctt gtgttgtaac ttgtggaaat
    11641 atagttggat ttcaagaagg cgtaattgat atgagtggtc ctactgctaa atatacacct
    11701 ttttcaaaaa caaataatat atgtattgtt ataaaagcaa aggatggtat agatactcat
    11761 gattatgaag aagcagctag aattgcagga ttaaaaatag gtgcatatgt aggtgaggca
    11821 ggaagagaag ttgagccaga tgaagtcatt gtatatgaga caaagccatt attaaaacaa
    11881 gtaaaagaat atccagacct gccaacagta gcatatgttc acatgttaca atcacaagga
    11941 cttttacatg acacttatta ctatggggtg gatgctaaac agatagtgcc tacatttatg
    12001 tatccaactg aaataatgga tggagctata atatctggaa actgtgtagc accatgtgat
    12061 aaggttacaa cattccatca tttaaataat ccagttatac atgacttata taaaagacat
    12121 ggaaaagatt taaactttat aggagttatt ttgactaatg agaacgtatt cttagtcgat
    12181 aaagagagaa gctctgatat ggtagctaaa ttaatagaat ttttaggagt tgatggagtt
    12241 ctagtgacag aagaaggtta tgggaatcca gatactgacc taatgatgaa ttgtagaaaa
    12301 tgtagtgaag ttggagcaaa tgtagttttg ataacggatg aatttcctgg aaaagatggc
    12361 aagagtcaat ctatagcaga cgctactaag gaagcagatg cagttgtttc ttgtggtcaa
    12421 ggaaatctag tggttcattt cccagcaatg gaaaaaatta taggaacttt agattatgtt
    12481 gaaatgatga taggtgggta taaaggatgc ttaaatgaag acggaagcat ggatgcagag
    12541 ctacaaatta taatagcatc aactatagca aatggctata atcatttgac agcaagatat
    12601 tattaaataa gtatttgaat ataaagttct gttgatgtaa tcattggtct aactgatgta
    12661 ataactataa agtgatttgg aggattaaga gatgagtaaa atcagagtgg ttcattatat
    12721 aaacaatttc tttgctggta taggtggaga ggaaaaagca gatattccgc cagaaaaaag
    12781 ggctggtgta gtaggacctg gaatagcatt tcagagtcaa tttaatgatg atgcagagat
    12841 aatctcaact gtaatttgcg gagatacgta ttttggtgaa aatattgaga gtgcaactca
    12901 agaactttta aaaatgataa aagaagaaag ccctgattta tttattgcag gcccagcatt
    12961 taatgcaggt agatatggag tagcttgtgg gactatttgt aaggcagttg aagaagagtt
    13021 aaatattcct gtaattacag gcatgtataa agaaaatcca ggtgtagata tgtttaaatt
    13081 agatttgcat atagtgtcta caggaaattc agcagcaagt cttagaaagg tagttccaat
    13141 aatgacgaaa ctaggtctta aattggttag aggtgaggaa ataggacctc ctgaagagga
    13201 aggatatata atgagaggaa taagaaaaaa tttcttccat gaacttagag ggtctgaaag
    13261 agcaattgat atgttggtta aaaagatgaa aggggaaagc tttgaaactg agtatccaat
    13321 gcctgaattt gatagagtta cacctatgaa acctgttaaa gatttatcta agataaaact
    13381 tgccctagta acttctggag ggatagttcc aattgataat ccagacaaaa ttgaatcttc
    13441 aagtgctact aaatatggaa tttacgattt aacagatatg gattctatgt caaataaaga
    13501 atttacaact atacatgggg ggtatgacag agcatatgtc cttgaaaatc caaatttagt
    13561 tgtaccacta gatgtagtta gagagcttga aaaagaaggt gtaattggag aattagtaga
    13621 ttattttata actactacag gaacaggaac gagtgttggc aattctaaga gatttggaga
    13681 agagttttct aagaaattgt tagaagatga tgtagatgct gtaattttaa cttctacatg
    13741 aggtacttgt actcgttgtg gagcaacgat ggtaaaagag attgagagag ctggtatagc
    13801 agttgttcat atatgtacag tagtaccaat atcacttaca ataggagcta atagaattgt
    13861 tccagcagta gggataccat atccacttgg aaatcctatg ttaggagaag aggaaagtaa
    13921 aaaaattaga aagaatatag ttttaaaagc tttaaatgca ctaacagaag atatagaaga
    13981 acaaacagta tttgaataat aaaaatacaa cacaacactg aaataaaaaa agatatcaaa
    14041 aaataatttt ttaaaattat tttacgatat ctttttttat taggtaaatt cgaaatttgc
    14101 att
//
LOCUS       DAYASV010000100        14027 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYASV010000100 DAYASV010000000
VERSION     DAYASV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.101"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            803..2158
                     /locus_tag="ACGI68_002779"
     CDS             803..2158
                     /locus_tag="ACGI68_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0139206.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
     gene            complement(2384..3265)
                     /locus_tag="ACGI68_002780"
     CDS             complement(2384..3265)
                     /locus_tag="ACGI68_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFH0139207.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDTSNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNNITS
                     VKIGGSAYTVLSGEIMY"
     gene            complement(3252..4004)
                     /locus_tag="ACGI68_002781"
     CDS             complement(3252..4004)
                     /locus_tag="ACGI68_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFH0139208.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            4262..4588
                     /gene="trxA"
                     /locus_tag="ACGI68_002782"
     CDS             4262..4588
                     /gene="trxA"
                     /locus_tag="ACGI68_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFH0139209.1"
                     /translation="MYIMVKIVNNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYE
                     ALGNEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKN
                     KIESYM"
     gene            complement(4795..6069)
                     /locus_tag="ACGI68_002783"
     CDS             complement(4795..6069)
                     /locus_tag="ACGI68_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFH0139210.1"
                     /translation="MQFMDVSKYIEGANLKVKTKSGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPLLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGISPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            6950..7786
                     /locus_tag="ACGI68_002784"
     CDS             6950..7786
                     /locus_tag="ACGI68_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0139211.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            8139..9716
                     /locus_tag="ACGI68_002785"
     CDS             8139..9716
                     /locus_tag="ACGI68_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFH0139212.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSNFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            9709..10893
                     /locus_tag="ACGI68_002786"
     CDS             9709..10893
                     /locus_tag="ACGI68_002786"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFH0139213.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIEKR
                     IL"
     gene            10911..11510
                     /locus_tag="ACGI68_002787"
     CDS             10911..11510
                     /locus_tag="ACGI68_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFH0139214.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            11534..12004
                     /locus_tag="ACGI68_002788"
     CDS             11534..12004
                     /locus_tag="ACGI68_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0139215.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEALVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
     gene            12363..13109
                     /locus_tag="ACGI68_002789"
     CDS             12363..13109
                     /locus_tag="ACGI68_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFH0139216.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            13170..13844
                     /locus_tag="ACGI68_002790"
     CDS             13170..13844
                     /locus_tag="ACGI68_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0139217.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKAEELADWISEYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLKCMMSCPWSL
                     RYANQK"
BASE COUNT         5166 a         1568 c         2294 g         4999 t
ORIGIN      
        1 taatttgaat ttttctaatt atttactgtc gattaataga tttttttctt gcatataagt
       61 gcatatttat ggtacgattt acatggtgaa ataaaaaagg ggaggtgaaa aataaatatt
      121 cgtgaaaaag ttttcagttt gaggtatttt atgaataata tattttgagg tattttataa
      181 ataatatatt atacataaag agtatagcct caataaatag tatcaaagcg aatatatggg
      241 taattacttt aaaataacag tttttatgtt tagattcaca gtgtttatta taaactaatg
      301 tgtaagaaat aagcctaatc tgtgaataga tagtagttta gttatttaag ttgagaattg
      361 gttgggtgaa gtaatatata aatttaaaaa ataaataaaa acattatttg aacaaaatat
      421 atgttgcttt attcgagact cctttaaaaa agcactaaaa tgtaattaca taagttgcta
      481 caaaaggggg attttagttt gtaaaatact atcacgtaaa aaataagaat tgcatatggc
      541 gaaggttttt tcatggtatt agatgagaat atattaattt ataactatat ctgaaaatag
      601 tgaatttata taatttaaat ttatgattaa gaagtgtatt aattttattc catgagaata
      661 tgtactctac aaataaatgc gcaattatct taataataat tacaaattgt tttacaaaat
      721 aataatatta tatctttcat aaaagaaaga atttggacaa aacgtaaaaa ataaaaatac
      781 aattaagaaa ggatttaaaa ttatgagctc aacagtaaaa aagaagggta aatttcctat
      841 gggattttat atctgctcaa caactttctc atttgaaaga gcagcatatt atgcttcaaa
      901 gtacttaata tatatattcc ttacaacagc tatagttcat ggtggtcttg gaatagataa
      961 aggacaagct gctataatgc aagccaacct tgttgcattc acataccttg cacctataat
     1021 tggggggtat atatcagata gatggatagg agctagatat actataccta taggtatgct
     1081 tatcatgggg gtaggatact atcttggttc tattgcaact acagtttcta tggtaaatgc
     1141 aatgataatc ttagtttcaa ttggtactgc attctttaaa ggtaacgtat ctgcggtaaa
     1201 tggacagctt tttgatagtc aagaagaact tgatacagca ttttcagtac aatattcttt
     1261 tgttaatata ggttctttta ttggtactat agcagttggt attctttacc ttaaaacatt
     1321 tgcaaaaaat ggtgtacttg gattttctca atgtttcttt atagcagctg tactttgtgt
     1381 aataggtgca atatggttca tatatggatg gagattcctt gggaatgcag gaaagagacc
     1441 ttttaaagaa ggtgtagttg ctgaaaaaat tgaagaaaaa gataagtctc ctcttacaag
     1501 catggataag aagagaattt gggctattat acttatatca ttcttctctg taattttctg
     1561 ggtattctgg tatctaactt atcttgcagt ttatgattat ggtgcagcat ttgtaaacat
     1621 gaatgttgga ggatttgatg ttcctcttgc ttggtttgac tcattaaatt cacttgtatg
     1681 tatagtatta ggaccagttc ttggagcatt atggtttaaa cttgctagta gaccacaagg
     1741 agatatgagt ttatttaaaa agacaggtct tggattaata ttcttaggtc ttgcattctt
     1801 aatgcttgtt ggtgcagaat tttcaagagg agttggagct cctgaaactg ctaaagctag
     1861 tatactatgg ataatcatgt ttggtattct tttaagtctt ggtgaaatgt tattctcacc
     1921 acttggtaac tcttttgtaa gtaagtatgc acctaagaag cttctaggtg ttcttatggg
     1981 agtatggaca tttgctactt ttatagctgg taaaggatat ggatatatat atgcatttac
     2041 tcttaaattt gatatgatta aagtatatac tataattcca gtaatactat ttgttgcagc
     2101 gatattatta ttcttatctg ataagacatt aagtagactt gttgaggacg ataaataatc
     2161 agtaagcaat tattatgttt tataggttaa tctactgtaa ttatgttgta ttagattgat
     2221 ttataactaa ttaataataa aaataaaatt ttacttctaa atacaatatt atgatatata
     2281 taattataat atttgttaga gtatgattat taaataaaaa taacaaataa aaatactagc
     2341 tttagttagc tagtattttt atttgttttt attaggtatg ggattaatac ataatttcac
     2401 cacttaagac agtataagca cttcctccaa tttttacaga agttatgtta ttgttttctc
     2461 tagttatatt tacatatacc tttccaggtc tgtttataaa gttaccttgc tcaccaataa
     2521 aactagtttt atctaaaata ttgtgatgta ctatataaga tgatacgcaa gcgttcccag
     2581 ttccacatac agggtcttct gtaactccaa catatggtgc tatagatctt aaatggtagt
     2641 cacacttttt atctatagtt tctatacaaa atggagttat accaattata ttattatcac
     2701 cactaaattt tttgatttta tttaaatctg gagagatatt ttttaaatca tctaaagatt
     2761 ttagaccaaa tacaagccac caaagtccag tatttacctt tataatagga tattcaagaa
     2821 gtctatcttc atgcacacct atcatttgtg caagttcttt tctgcaatta tctaaatttt
     2881 caaattcagg tgtattctgt tccatcataa atatattttc attatttatt ttcttaagct
     2941 ctacagataa tacaccagct tttgtttctt gataaaatat acttttatta gtattaagct
     3001 tcccttcttc caaaagtgca tggaaagtac ctattgttgc atgaccacat aaatcaactt
     3061 ctaaacctgg tgtgaaaaat tgtactttaa aatctgcaat atttgactct gaaacaaatg
     3121 cagtttctga taaattcatt tctttagcca cattctgctt aatttcatca gaaatgttag
     3181 aggtatcagt aacgacacca gcaggatttc caccaaaagg tatagttgta aaagcatcaa
     3241 cttgtttaat cttaattttg ttcattttta taacctcctt ttatttgttc tgaaatataa
     3301 atttcatgtt ttatgtattg aactccaata tataaaatgt tagagttttt tgtccaaatt
     3361 gaaatggcct cactgccttc tgataagttt cctgtcaata tttcatcatt atctacaatc
     3421 aaaataattc ctttgtaaag cattttattt tcaagtgtag ttttattacc tccatgaata
     3481 tgaatattat caagaccttt cagacttttg ttttctaaaa gtagtatttc aatattaata
     3541 ttttttttgt aagcaattag gagttctttt tttaattcaa gaatttcttc ataagggcaa
     3601 gaaattataa tttcttgttt tgcatttttt atcatttcta ttgctttttt taatatattt
     3661 ttatctcctt ttatgtttaa tacgtattct acacttttac ttttatttaa tatctgtaag
     3721 ctattcttta gattatccat agaattgtca aattcttttt tcttattttt taataattca
     3781 ttagggttta ttggtatgta cttaataggg tcagttccaa cattgataat tatattattg
     3841 tttaatagct ttgttatatt ttcatagact tttgattgtg gtacacctga taatttactt
     3901 atttcatatc ctgttaaagg gctttccttt aataatgata tgtatatttg agattcgtat
     3961 ttagtaaatc ctatattttg taattcatta ataatattat ccatatagaa actcctttga
     4021 taaaagatta gtaactacct atgtagttat taatttgatt ttataattta agggaaaaaa
     4081 tgtcaactat attaagaaaa aaataaaata tattagaaat atttagtctt ataaatattt
     4141 aatcttataa atctatttat cataatatta aatctattaa aaataggcgg ttacaaaaaa
     4201 gtgcctaatt gaactgttat ataggtcgta gtatattaat atcaagaaga taataaagga
     4261 gatgtatatt atggtaaaaa tagtaaataa taatgaattt ataaatgaag tagaaaataa
     4321 agatggttta gtagttgtag atttctttgc gacttggtgt ggaccttgta agatgctatc
     4381 accaatatat gaagctttag gaaatgagat ggttgaaaaa gcaaacttct tgaaggtgga
     4441 catagaccaa agtatagaat tagctcagaa atttgaagta tctacagtac caactatgct
     4501 aatatttaag gatggaaaac ctgtagatag attaataggt tttatgccta aggagaattt
     4561 aaaaaacaaa attgaatcat atatgtaatt aaagtaaatt gaagtaatta aaataaatct
     4621 catatataaa caaaaaaact cattgtaatg agttttttgt ttgtatagtg taaaatagtt
     4681 tgtatagtta caaatgagtg tctaattgct aatgaaattg actgttgata taatttgctt
     4741 ttttgtgaaa acaaaactac tctctattta ttgataaaaa agaatagaaa aaacttattt
     4801 aaaagccaaa gttttaatag catgtataaa gctatccacc tcatcaaaag tattgtacat
     4861 tcctaagctt atcctaacaa gacctacagg attttccctc ataaatctga catatgcttc
     4921 ttttggactt atacctaata atttttgtac ataaatatct gcacctacta ctccagctcc
     4981 agtttctatt ccaaagttat caactaaata ttttgctaca tctttgtaat acatattact
     5041 tatattaaaa gatatatttg ggataattac attatttgat ttatctccat atattattat
     5101 attaggtatt ttactcattt cttctttagc atataaaaat aattctcttt cgtattttga
     5161 aattctgttt agaccaattt tatttaaagt gttcagtgct tttcccattg caagagttcc
     5221 aaaatactct gggtatcctg cttcaaaacg ttggggtacg tttgcatata gtattttgtt
     5281 atcatttaca aatttggtag aacctgagcc atatagtaca ggattaaatt tttcaaagaa
     5341 ttttttaggt cctaccagag ctcctccatt tagtggagaa taacatttat gagcactaaa
     5401 aacaacaaaa tctatatgct catcacattc atgtggcatc attgaaaaag gtttatgctg
     5461 aattacttgc acaatatcca ccaatatctt tgcaccatac ttatgagcta ttcttgctat
     5521 ttcatatata ggagtggtta tacctgtgac attactagct cctgtaactg taacgagaga
     5581 tacattacca gcataatttt taagtatatt ctctaattga cataaatcaa gttctccttt
     5641 ttctgtaatt ccaacaactt ttgtaggaaa attaacttta aaaggcaggt aattagccat
     5701 atgctccatt gctgtggtaa taacaaattt atctggattt tcatctttaa aaaattttgc
     5761 taagaagttt attccttctg tagcattttt gacataaata acagaatcct tttcttcgtc
     5821 tccatcaata agtttgagga tagtagagcg tacattttca tattttaaag tatttttttc
     5881 tgagattatt cctggagcat taatataagt aagcaaagga atctcacagt ttaagttatc
     5941 cactacgttt tttaaaacta atggagtggc agagttgtta aaatatattc ttttaacagt
     6001 ttcaccactt ttagttttaa ctttaaggtt tgcaccttca atgtatttag atacatccat
     6061 gaattgcata aaaaaacccc ttatctaata aactattctg ttttttatta aaaaagctca
     6121 agttttaatc aagagcttgt ttaatatatg ttatgttttt ataaggggtt ttgttaatta
     6181 attcttgttg agatgtttat taaattacac gaaaatgagc tacagttggg ggaaactgta
     6241 gctcacttga ttttggggga gataggttga acttattgtt tatccgtatc atatttttag
     6301 tatagtcact tattttaatt tatatacatt tttttataaa ttttacataa aatgttaaac
     6361 actaattaga aataattatg aatatgacta ataaagttgt aaaaatagta aagaaattga
     6421 ttagaacata atggaaaata tattgtatat aattagaaaa aaagatttta tatgttataa
     6481 aagatgtgca agtataataa ttttataaaa aatacaaaat ataatacaaa taattttata
     6541 aaaaaaatta taaaaaaatt taatttatat aaattaaaaa ttatattacc aatgtatata
     6601 tttgagaaaa gattagtaca aagctataat ctgaatttca ataaatatat atataattaa
     6661 gttgaaacac ttgaaaatac tggaaaaaat tgaaaatagt ttttgtaata taaaatgtcg
     6721 attgatttta aataattaaa tatacataag aaataattaa atatatttca atgaaaatat
     6781 gataaaatat gacaaattaa ttgatgacaa taaaaaatat tttgttattg tagtattata
     6841 gggtaaattg caaaatatat acgaaagtaa aattttaaat attgacatta aattaaatat
     6901 actatactat gtaagtgtga tgagagaatt tttttagagg ggatttatag tgaatataaa
     6961 aaagatattt aataacaatg tagtagtaag ctccctagaa gatggcacag aaataattgt
     7021 cactggtgct ggagtaggat ttaagaaaaa agtaggcgat ttgatagatg aaaatttgat
     7081 ttctaagaaa tattttgttc aagacgatca aagggacaaa tacaatcaaa tacttaataa
     7141 gacctctatt gaatacttta aaatttcaga agaaattata gaaaaggcta atgaagtttt
     7201 aaatactcaa gtgaatgatt caataatatt agccttaact agtcacatag aatttgctgt
     7261 ccaaagagaa aaacaaggta taaaattacc aaacttaata ttgaatgaga caaaacaact
     7321 ttatagggaa gaatttgaat ttggattatg ggcaattgat gaaattgaaa aaaggatagg
     7381 aataaaattg cctgaagatg aagcaggata tattgctatt cacataatca atggtttgga
     7441 aaattctcaa aaagatgaag gtattaatat actagagttt tcaaaacaag ttattcagat
     7501 aattgaagaa gtacatggat ttaaattgga tgtaaattca ttaaattata ctagacttat
     7561 aactcactta aagttttttg ttcaaaggat tctccgaaaa gaaacttaca atgatagtga
     7621 tattgaagat atgtataagt tagtgaataa aaattacaat aaacctaaag tgtgtactga
     7681 agaaattgca gcattggtat tagacagatt tgaatataag attagtaaag aagaagaatt
     7741 atatttaatg attcacataa ataaaataac aaacagtggt tgttaggatt gtaactgata
     7801 aagcaggcaa aacctaagtc attgagctta actattagat taagtttttt gatttaggtt
     7861 tttttattag atacaatcaa aatacagaca gtgactttta ggattgtaac tggttaagca
     7921 ggcaaaacct aaatcattga atttaactat taggttaagt tttttgattt aggttttttt
     7981 attagataca atcaaataca gacagtggtt gttaggattg taactggttg agcaggcaaa
     8041 acctaaatca ttaaacttaa ttattaaatt aagttttttg atttaggttt ttctataaat
     8101 ttgattatgt ttttagataa aaataggagg ggaaaattat gaaaaaaaat aagttaggtt
     8161 tatggaactt cttccaaatg ttaggaaaaa cgtttatgtt cccaatagca ctacttagtg
     8221 tttctgggat gatgctagga cttggggcag gttttacaga ccctaagatg attgaaatgg
     8281 ttccattttt agggaatgaa tatgttaact tagttttaaa cttcatgtta acaataggat
     8341 tatttgcatt taacaacttg ggagcattat ttgcaatggc aattccacta ggattattaa
     8401 aaaaagaaaa agagtttggt gctttttcag gattagtagg atttatagca atgcatatag
     8461 gtacaaattt ttatttaaca agagctgatt tgttagtacc agcagaacag atgtcaacta
     8521 atggtcaagc tatgattatg ggtatacaaa catataatac aagtgtttta ggtggtattg
     8581 tagcaggatt aattgtttat gcaatatatg ataaagttag taatttaaag ataccagaat
     8641 cattaggatt ctacagtgga ccaagattag ttccaattgt tagtttaata gttatgagta
     8701 tagttggatt attaatacct attgtttggc caccattttt caatgcattc caaagtttag
     8761 gaagatggat ttcttcagca ggaccattag gatatttttc atatgcagtg gcagaaagag
     8821 ttacaattcc atttggacta aatcatcttg taacatctgt attccgtttt acaccaatag
     8881 gtggaactgc tgttattgat ggagaaacgt actttggtac attgaacatg tttatggcat
     8941 atgtagaaaa taatcagatt atacctcttg atttagctgg taaaatggaa caaggtaaat
     9001 tgatgattca atatggatta gctggtgctg cattggctat ctaccgtaca gcaaaaccag
     9061 aaaatagaaa ggcacttaaa ggacttttaa tatcaggagt gttgacagtt attataggtg
     9121 gtataagtga accaatagag tttctattct tattcatatc accagcatta tttgctttcc
     9181 atacatttat gaatggtcta gcaaacatgg tacttccata cttaggcgtg ttgatgggat
     9241 tcacaggaga tttaattgca tttataagtt ttggtgtgtt aagaggaaca gctacaggat
     9301 ggccaatagc agtattagta gcagttttat attttgcaat atactatttt gtatttaagt
     9361 gggcaattat taagtttaat ataaagaccc caggacgtga ggataaagaa ataaaagtat
     9421 ctgaaggaaa tggttcaaat ttcaaaaatt tgtctacata taaaggacaa caaatgatat
     9481 ctgctttagg tggtcaatct aatatattat cactagataa ttgtgtaact agattacgtt
     9541 taaaattaca agatgtatca ttaataaatg aggatgcaat taaagaggct ggtggtatag
     9601 cagttgttaa attggatgag catacaatac aagtaattat aggaacccaa gtgtattctt
     9661 taagaaagca gatggataaa gctatggaag gtcatttgga ggcatgtaat gattaatttt
     9721 gataaaaaag tagaccgatt gggtacatat tgtacccagt gggactatgt agaagataga
     9781 tttggtaaga aggatttgct tccttttaca atttcagata tggatttaga gtttccattt
     9841 gaaataacag aggtgttgca ggaaaggtta aatcatagag ttttgggata cagtagatgg
     9901 aaacatgatg attttagaaa ttcagttacg aattggtata ttaaaagatt taattgtagc
     9961 gttgatagta actggatata ttatagtcca agtgtaatgt attcaattag caaacttatt
    10021 gagatgttaa gtaatgaagg agatggaatc cttataaata ctcctgcata caatgctttt
    10081 tatgatgtga taataaataa caatcgtaat attataaagt cacatcttgt aaataggaat
    10141 ggattctatt caattgattt taatgatttt gagaaaaagt gtaaaaaatc taagatattt
    10201 attttatgta atcctcacaa tccaactggt agagtatgga gtgagttaga acttgttaag
    10261 atgataggaa tttgtaagaa taatgatgtc aagataatat cagatgatat acacatggat
    10321 attgtatatg acaatagaat gacacctatc ttaaatgttg caaaggacta tttagattct
    10381 gtattcattt gtacatcagc gtctaaatct ttcaatatac cagcattgac tgggtcatat
    10441 gtgattatac caaatgaagg tattagaaat aaatttgaaa atatcactag ataccgtgat
    10501 tttgtaaatt cgccagcaat tctagcaata ttagccacaa tggtctgtta tgatgactgc
    10561 gaatattggt tagatgaact attaaaatac cttaaagaga atcttaaata tacgattgat
    10621 tatataaaag ataatttaga accattgaaa ttggtcatgc cagaaggctg ttactttgct
    10681 tggatagatt tttccgaact taaaataagt ggtgagaaat tccaaaaatt attgatggat
    10741 attggagaag ttgctattat gtcggggtct gtatatggtg aagaaggtga gttttattta
    10801 aggttaaatg tggcatgttc aagagaaaaa cttgaagatg gtttaaatag gattaaaaaa
    10861 actatggatt atattgaaaa aagaatttta tagtaggaag ggagatagtt atgggatatt
    10921 taaaaacaat ggttgatgtt atgactactg aaatgaatgc cataagatgt ttaatagatg
    10981 aagcaggaat agaatatgaa agtatagtta gtgaaattgc aaattgtgaa ggcaaagtaa
    11041 tttttatggg tgtaggaaaa tctgcacata taggtaagaa attggcagct acatttgcta
    11101 gtactggtac acctagcttt tttgtacatg caacagaggc agtgcatgga gatttaggaa
    11161 tgattgaaag taaggatatt acaattttaa tatcaaatag tggaaattct atggaagtgg
    11221 taaactgtat aaaatatatt aaagcaatag gttctaagac aattgcattt acttcaaaca
    11281 gaaattcagt actggcaaaa gagtgtgatt atgctttaat atatcctgca aaggatgaag
    11341 cagaccattt aaatctagca ccaacaacat catctacaat tactttggtt ttaggagatt
    11401 ctattgcatg tgcattatct aagagtagta attttggttc ttcagatttt tataagtacc
    11461 atccaggagg aagtttaggt gagaagttaa aaacagctaa caatggataa aagcaaaaag
    11521 gagagtagtt tttatgttca aaatgtttaa aaaaaaatca ataaaatcac ccatatcagg
    11581 aaaagtaata gagttatcta aaatacaaga tggtgtattt tctcaaaaaa ttatgggaga
    11641 gggtgtagct atagattcta caggagacat tgtatatgct ccaatcaatg gaaagatagt
    11701 agtagtagca gagactaaac atgcatttgt aatagaatta gaaaatggaa tggaacttct
    11761 tattcatgtt ggtttagata ctgtaaattt aaaaggcgaa ggttttgagg cattggtgag
    11821 tatgggagaa caagtgaagg aaggaacacc aatgttgaaa attaaccgtc ctttgattga
    11881 atcaaagggt atatcactta ttactccagt tacaataact aatcattcag agtacaatat
    11941 gaatacatgt aatgtaggca atagtgttga aggtgggaaa gatactgtaa ttgagtttaa
    12001 ataaagaact aaaatacaag gttaatattt ataaatacct ataaacttat gttttataag
    12061 attgattgta tagatttgtt gtataaaaaa gaactcttta attataaatt aaggagttct
    12121 tttttatttt gtaaatattg tttagattta tatagaaaaa cacttagtaa taaatagaat
    12181 aaataagtat ttatatagct aaaaatctaa atagttttgt ttaaatatat ttttatattt
    12241 tgtaatttca aattaataaa taattagatt tgataaacaa aaaataaaaa tgttgattat
    12301 attaataaac tttaatataa tatatttatg aaaaatgtaa actgtgaaat ggaggtagta
    12361 acatgaaaat tgaaaatctt tctattgatg atattaaacg aggctatata tttaatgatg
    12421 atttaaattg ttatagttgc attaaatgta caaaaacata tgaggtagga gaaatatata
    12481 aaataggtaa tagattcttt gaagcttcaa aagctattga acttcatatg gaattagaac
    12541 atagtgatta tttagagcaa ttgcttaata gtgattcaaa atacaacaca ttgactgaaa
    12601 atcaaaaaca attatttcaa ttatttatta gtgatttacc agataaggag atagcaaaac
    12661 aggttggagt atccacatct actatacgcc atcaaaaatt tattttcaga gaaaaagcaa
    12721 aacaggctaa gctctattta gcactatatg aatctgtatt tgaagataaa tctaataaaa
    12781 gtagtactat aataccaatc catgaacatg ctcgaatggt agatgaaaga tatgtagtaa
    12841 ctgaagaaga gagaaaacac attttggata catcatttga aagtatgaat cctttaaaat
    12901 taaaagtatt ttcttcaaaa gaaaagaaaa aggtcgtaat tttatctaag attttggaac
    12961 aatttgaacg tgggaagaaa tattcagaaa aggaagttaa tcagatttta gaatctatat
    13021 atgatgattt tgcaacaatt agaagatatc taattgagta tggttttatg agtagaaata
    13081 aagaatgtac aaaatattgg ttaacataat taaaataata aattttacta atgtgtaatg
    13141 ttatttttat ctagggggag gagtcaaata tgaatgatga attaaaatct attttataca
    13201 ataaaggtgt ggacattgtt cgctttgttg atatatctga atttccaata aatcaaacgc
    13261 aaggtttttc taaagcaatt ttattttgta tagggttatc aaaaaaattt attacagata
    13321 tatacaataa cttacctaca gatagtgatg agtttttaga aaaagaggag aaagcagaag
    13381 aattagctga ctggatttca gaatatattc aaaataaagg atatagagcg tattctcaat
    13441 cggagaaaaa caatctagaa catggatatt ttgaaaaagc ctatattaat ccagaaatgc
    13501 aatcagggat tagtccttta ccacataaga caatagcaaa tattagtggc attggattta
    13561 tgggaaaaaa taatttgttt gtaacagagg aatacggatg tgcttttagc atgtgtacag
    13621 tacttacaga tgctccaatt tctgttgaaa gatatccttt gattgattca aaatgtatgg
    13681 attgtaatgt ttgtgtagaa aactgccctg caaaagctat acatggaaat gagtggactt
    13741 tgccaggaaa aagagagtct attattgatg tttcaaagtg tttttgtgta ttgaagtgta
    13801 tgatgagttg cccatggtca ttaagatatg caaatcaaaa ataattttac tagagaaggt
    13861 attaaattga atattataaa atttttaaaa tgtaagatta tataagattt ttcaaaataa
    13921 aaatttgtat agaacactca gaaatgagtg tttttttatt ggaattaaat tatattgata
    13981 caaagtttaa ctattttaat cattatataa aatttgtatt aaaaaat
//
LOCUS       DAYASV010000101        13308 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYASV010000101 DAYASV010000000
VERSION     DAYASV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.102"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            98..1528
                     /locus_tag="ACGI68_002791"
     CDS             98..1528
                     /locus_tag="ACGI68_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139218.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1631..2734
                     /gene="hisC"
                     /locus_tag="ACGI68_002792"
     CDS             1631..2734
                     /gene="hisC"
                     /locus_tag="ACGI68_002792"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0139219.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENRKFVAVLKEILNIKGEPSIG"
     gene            2767..2985
                     /locus_tag="ACGI68_002793"
     CDS             2767..2985
                     /locus_tag="ACGI68_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0139220.1"
                     /translation="MNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAHVEVDES
                     KCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3002..4051
                     /gene="vorB"
                     /locus_tag="ACGI68_002794"
     CDS             3002..4051
                     /gene="vorB"
                     /locus_tag="ACGI68_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HFH0139221.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4053..4793
                     /locus_tag="ACGI68_002795"
     CDS             4053..4793
                     /locus_tag="ACGI68_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFH0139222.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            4796..5335
                     /locus_tag="ACGI68_002796"
     CDS             4796..5335
                     /locus_tag="ACGI68_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HFH0139223.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5459..7558
                     /locus_tag="ACGI68_002797"
     CDS             5459..7558
                     /locus_tag="ACGI68_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HFH0139224.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMEKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(7825..8340)
                     /locus_tag="ACGI68_002798"
     CDS             complement(7825..8340)
                     /locus_tag="ACGI68_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HFH0139225.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8432..9643)
                     /locus_tag="ACGI68_002799"
     CDS             complement(8432..9643)
                     /locus_tag="ACGI68_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0139226.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKFLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10319..11761
                     /locus_tag="ACGI68_002800"
     CDS             10319..11761
                     /locus_tag="ACGI68_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0139227.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            11803..12156
                     /locus_tag="ACGI68_002801"
     CDS             11803..12156
                     /locus_tag="ACGI68_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139228.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12347..13168
                     /locus_tag="ACGI68_002802"
     CDS             12347..13168
                     /locus_tag="ACGI68_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HFH0139229.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKAYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         5040 a         1564 c         2417 g         4287 t
ORIGIN      
        1 tactcctgaa tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata
       61 ataaaaagta agtaaatttt ataagggagg ggtagatgtg agtagtcaag aaagcaaacc
      121 aaaaggaaat cttatatcaa attttagaga tgatatgaga gaccctaaga aaagagcaat
      181 atacacatat tcaagttcta taaatttagc gtacaatttt ataggttact ttgtatcaac
      241 tgttacaaat atttttttaa ctgatgtggc aaaacttggt gtaattatgg caggtggtat
      301 gcaaattata caatctttca taaaaattgc tgtagctcct ataacaggta cagtatttga
      361 caaacaacca ttcaaaaaag gaaagtatta tccatggctt agatatgcac cagcagcgtt
      421 agcattaaca tatggaatat ttttcatagt tccattaatg aaaataagtc cagcattact
      481 tttacctttg atgacagctt tattattaat agcatcttgt gctcagcaag tagttttcac
      541 tataatttat gctatttacc ctgtagttgc taagacacct agagaccgag ttataggctc
      601 aactactacg aatatattta aagaagttgg taaattctta gtaggttttt catatccgtt
      661 attattagtt ttatttgcag atgtattagg tggagaagga atgggttatt taggtaccta
      721 cttattcttt gcagcaggat gccttttagt attttggttt tcttcaacag agataataaa
      781 atctggagca gaagaagaag ttcttcaaaa agaagagaag ccaaagatta aagcaagtga
      841 aatggcaaaa gctttattca ctaatgtacc acttatgatg gcattttgtt tagaatttct
      901 tatatgtata agatctatag gattaggacc tttggctcca tattatttta aatatgtaat
      961 agaagatgaa cgtggattag cagtattttt atcaatcatg cctttggtaa gtgtagcatt
     1021 catgtttttc gcaccagtgt ttataaaaat ctgtagagag caaaaactag cttcaataat
     1081 ttctttttct atatgtgcac tatgccactt attggttgca tggactcctt gggggaaaac
     1141 tactttagga gtaactatgt tgttggcaat tggtggaggt ttttcaaatg tagtatcaat
     1201 aataaatctt aatttctttg ccggttcttg tgattatggg cattggaaat caggaaaaga
     1261 cttacctggc ttaagtatgt cactatatcc agttgcaata caagtaggag tattacttgc
     1321 aactactata agaacagttt taatgaactc aatgggatat caagctgata tggtagtcac
     1381 agaagcagtt aaatcaggtt ttataaatat gatttcttat tctatggcaa tacctttaat
     1441 aatagctgtt gttatagcaa tactatatcc agtaagtgac aagaaattaa accaaataag
     1501 agaagagtta aatcaaagaa atgcttagtt aaaagaaagt tgagtagatg gttaatttaa
     1561 gctattaaag taagtagacg caatagcgtt tatataaatg aaaatacata tacagagggg
     1621 gcttttaaaa atgatagaaa aattattgag aaataccgta aaagaattac atcaatatgt
     1681 tccaggtgaa ccaatagaaa aagttaagga aaaatatggt gtaaaagaaa taataaaatt
     1741 agcttcaaat gaaaatcctc taggaccatc accaaaagca atagaagcta tgattgaaat
     1801 gttaaaacaa ggtcaacttt atccagagcc agaagctaac gaacttagaa gaaaattagc
     1861 tgaaaagcta gatttaaaac cagaaaattt tattgttgct aatggtgcag ataacgttat
     1921 cactctaata ggtgaggctt ttataaatag aggagatgaa gttatatatt gtaacccaac
     1981 atttccatca tatagaactg caactataaa aaatgaaggg ataccagtag aagtaccttt
     2041 aacagaagat tataagtatg atttacaagg tatattagat aagattacag ataaaacaaa
     2101 attaatatgt gtatgtaacc ctaataatcc aacaggaaca attgtggatg ataaagagtt
     2161 agaagaattt ttaaagaaag tccctgaaaa tattataact atattggatg aagcatatat
     2221 agaattttta acagttccta attatgtgga tggacttaag tatgtaaggg aaaattataa
     2281 cgttatagta acaagaactt tctctaaaat atatggtctt gcagggctta gagtaggata
     2341 tggtattgct aaagatgaga taataagaac tttatttaca gtaaaagagc ctttctctgc
     2401 aaatcgtgtt gctataagtg gagcaacagc agcacttgat gatgaagagt ttataaaaga
     2461 aacatatgaa ttaaatagag ttggaatggc ttactttaaa gaagagttta caaaaatggg
     2521 atttgatgtt gtagattcac aatcaaattt cctttatgta gatatgaaaa cagatatacc
     2581 taagttattt gaagacttga aaaaaagagg gtttgtaata agaccaagtg ctacacatgc
     2641 acgtgtaagt attggaacta tggaagaaaa taggaaattt gtagcagttt taaaggaaat
     2701 attaaatatt aaaggagagc caagtattgg ataatagata gaaatattat gaaacggggt
     2761 gtagatatga acttaaaaat aaacgcagag agatgtaaaa gttgtgaata ctgcgttata
     2821 tcttgtaaaa aaggagcatt aaaaatatca agtaagataa ataaagaagg ttatgctcat
     2881 gtagaagttg atgagagtaa atgcatactt tgtggaattt gctatcaggt gtgtccagat
     2941 aatgtttttg aaataattaa agaagaagca tgtcaagagg cttagaagga gttgggataa
     3001 tatgaaaaag atgtggaagg ggaaccacgc cattgcagaa gcagccctaa gaggaggttg
     3061 tgaattttat gcaggatatc caattacacc acaaacagag gtaatggaat ttttatcaca
     3121 tagaatgtct gaacttggaa gaacttttat acagtccgaa aatgagatgg ctgctatata
     3181 tatggtatat ggagcatatg caagtggaat gaggtctatg acgagttctt caggaccagg
     3241 aatatcttta aaacaagaag ggatttctta cttatgtgca aatcattacc cttgtgtaat
     3301 cgtaaatgta cagagatggg ggcctggtct tggctcattg gactcagcac agacagacta
     3361 tctaagagat actagagggg gaggaaatgg agattatcat ttgatagttt atgcaccaaa
     3421 cagtattcaa gagactgtag atttgatgta taactcattt gatgtagcgg agaagtacag
     3481 agtgcctgtc gaaattttga cagaagcagc tttaggtcaa atgatggaac cagtagaatt
     3541 tccagagttt aagaagagag aaggagattt aggttggact tatgatggaa gtaatagaga
     3601 ccatgcaaag gttgcagata accaaaagcc aactttttgt atggagaaac gtatgagaat
     3661 aactgaaaat gaacaacaat gggaagatta tcaagtagaa gatgcagaat atgtgtttgt
     3721 agcgtttggt ataccatcaa gaacaactat gaatgctgtt agaagactta gagaacaagg
     3781 tgaaaaagtt ggaataataa gaccaataac agtatggcct ttcccatata aagcatttga
     3841 aaaggtaagt aaagatgtaa aaggatttat ctcagtagaa tcaacagaca caggtcaatt
     3901 agtagaagat gttgctttag cctcaaagaa agtatgtaaa gaaaatgtac ctgtgtatgg
     3961 tttatttagt ggaaatcata taccaaaaac attgcaagta atggacactt atagcaaaat
     4021 aaaatctggc gaaatcaagg aggtgttcta atatggctac agaaaaatta acacctagcg
     4081 ttgtttcaat accaaacaag ttttgcccag gttgtggtca tggaatagta aatcgtataa
     4141 tagcagaggt aatagaagaa catggatatg aaaaaaatca tgtattaaca ttaggagttg
     4201 gttgtgtctg caatatgaat tttagctgga atggtgataa gatgcaaact gcacatggta
     4261 gagcttcatc tacagctata ggagttaaag tagcacttcc agatacatta gtaatgactt
     4321 accaaggtga tggagatgct tatgttatag gtctttctga aactttaaat acagcatata
     4381 gaaatcataa tgtgactgta tttgtaataa acaataataa ttttgcaatg actggtggac
     4441 aaatgtcatg gaccactatg ccgggtcaag tcacaacaac aagtgtaaat ggaagagata
     4501 taaagactac tggtagtcca ctgaaagttc ctgagatggt ggcaaacttc tttgatgtag
     4561 catatgtagc aagaggttca gttcatagcc caaaggaaat aattaatcta aaaaaatata
     4621 taaaaaatgc aatagaagct caattaaatg gagagggata ttccttagtt gaaatattag
     4681 caccttgccc aactaactgg ggtgtatcac tagagaaatc tatcaaatgg atggaagaag
     4741 aaatagtgcc atattatgct cttggcgaat ttaagcaaag ggatggtgaa tagttatgga
     4801 aaaagagcta gtatttgcag ggtttggtgg tcaaggagtt ttaactcttg gtctgattgt
     4861 agctgagtca gcacttgaat tggggaaaca agtaacttgg atgcctgcat atggtccaac
     4921 tatgagaggt ggaaaagcct atagtgtagt aaagttttct gatgagagta taggtgggcc
     4981 tgatatggaa gaaatagatg ttttagtagc aatgaataaa ccatctttag attatataaa
     5041 tttagttaaa gaaggtggaa cagtagttat taatacatct gcaatagatg agaatgtaac
     5101 attaagagaa gatataagtg tagttaaaat aaattgtcag gaattagctc aaaaagtaaa
     5161 caatccaaaa gcagcaaata tagtagtact tggagcatta attgctaaaa ctgggttatt
     5221 tgaaaaagaa ttagctttaa aaactatgtg tgatttcttt gaggaaaaag gaaaaggaaa
     5281 gtttaatgtt caaaatgaag cagcattcat ggaaggctat aataacgcca tttgataaat
     5341 tacccttatc ttaagtgatt tatctcaaga tttaataatc tttagtgcca agagaaactt
     5401 catcttgaga taaaattcac ttaaaatcac aactataaac atataggagc gtgaatttat
     5461 gagtttggaa aaacttatta gaccaaaaag tattgctata gtaggagtga ctgataaact
     5521 tggttttgga agaagtgcag cacttagtat agtaaaaagt aaggaaacag atagagtata
     5581 ttatgttaat cccaaaagag aagaattgtt tgggagaaaa tgttataaga cgatacaaga
     5641 agttccagaa gtagtagact gtgtagtagt atgtacgcct agaaatgtag ttccatctgt
     5701 attaaaagat tctggagagt taggaacaaa ggcagcagtg gtatatgcta gtggatttgc
     5761 agaggaaggt acagaggaag gtacagattt agaaaatcaa cttatagaaa tatcaaatac
     5821 atacgatatg aaaatattgg gtccaaactg catggggttg ctaaattgta tagataaggt
     5881 taatctatgg gcaggaggtt ctaaatggga tttagatact aaaaaacctg gtataggaat
     5941 tgtaggtcaa agtggattta taactgctga aattgtttca agtgattatt ttaatatatc
     6001 ttatggtttt tcaacaggaa atggaaatat agttacacta gaagatgcag tagatttcct
     6061 tgtagatgat gattatgcat cagtaatagc aatttacctt gaaggtttaa aaaatcctca
     6121 aaaatttata gatgctttaa aaagggcagc acaaaaaaga aagccagtaa taatattgaa
     6181 atcaggaagg tcagaaaaag gtgctatttc tgctgcttct catacaggaa atttagctgg
     6241 ttcgagtaaa gcttttgaaa gtatttttga aaaatatgga gtaatttctg tggaaaattt
     6301 agaacagttt atgtgtttag cacaagcttt tagtgtttta gatggtaatt tacctgcaaa
     6361 ttctaatttt gcagcaataa acttctcagg aggagaaaat actatttgtg cagatttagc
     6421 agaagaaaat ggtgtagaac ttgcagaaat atcttctgaa actaaagaag aaatggaaaa
     6481 atatcttcca ggatttgcaa cacctaaaaa tccactagat gctacaacag cattattcca
     6541 tgaaaaggat atgatagtag gtttattaca tacctttgaa aaggatgata gtgttggatt
     6601 tactatggta ggtgcaaata taagagatga agaaaatgaa atgcatgaaa ccctttgtaa
     6661 agctgtaagt gaagctagag aacaaggatt gaaaaaacct gtgtttgcag ttcctacact
     6721 tgaaggaaat agatatttag attataggac tagattagaa gataatcaag tgcctataat
     6781 gtcatcagtt ggaactacat ttacttgttt taataaaatg gctaagttta tagattatga
     6841 ttattcaaaa agaactttag aatttaaagc tgttaagaaa agagagtcaa ataatgtagt
     6901 agcattatca gaattagata gtaagattga gatgaaaaaa tatggagtac cagttccagg
     6961 acaaggtaat gcaaaaagta ttgatgaatt agatgaaatc ttaaaatata ttaagtatcc
     7021 tgttgtttta aaaataaatt cttctgaaat attgcacaaa tctgatgttg gaggagttaa
     7081 aataggaatt aagaatagag atgaggcagt agatgcatat aatgagattt taactaatgt
     7141 aaaaaaagca aaaccagatg ctaatattga tggtatatta gttcaagaaa tggtagaaag
     7201 tggtatagaa attattattg gtataactaa tgatgaccaa tttggaccaa tgttattagt
     7261 cggtttagga ggagtatttg tagaggtatt taaagatact acattatatc cattacctat
     7321 aaatcatgat gaagctatta tgatgcttaa aaaactaaaa tcatttaagc ttttaaatgg
     7381 ctatagagga agtgagccat gtgatattga tgctcttgca gatatgatgg tcaagttagg
     7441 aaaatatgct tatgaaaata aggatgaagt caaggaaata gacttaaatc cagtgtttgt
     7501 atatcctaaa ggaaaaggcg tatgtgcagt agatgcctta atagttaaat ataaatagtt
     7561 tataagtttt ggtattcaac cataattaaa gcaccaactg aaaatcacat tttgtaagtt
     7621 agatagtgtg attttcagtc accccaaatt taatttaaaa atattttaaa atgaaagtat
     7681 agttttaagt ttcattgtga aatagcactg tataaacaat aaaatactac gtataaaacg
     7741 aataataaaa ggtgttttaa tgataagaat caatcttgat tttcattaaa acaccttctt
     7801 tattatatta aaaattttta aacgttatat tgtcacaggt gggtttgttg gagctgcata
     7861 tgtacgatta gcaaattctt ggtcaacaaa gaatagccca ccctctccag caagtttaac
     7921 acgttttata ttgtcttcac aatttgtttc ttcttctgct tgttcagata taaaccaaag
     7981 taagaattga gaagttctaa agtctgattc agagtttgct acagctgcta agttgtttat
     8041 taaagctgta accttttttt catgagctaa cgtacgctca agaatatcca ttgcagaagt
     8101 aaagtctgaa tctggcattg ggatagcatc aagctgaacc tttcctccta cattatgtaa
     8161 gtatttgtaa aaaaacatag cgtggtctgt ctcttcttta tattgaacat aaaaccaatt
     8221 agaaaatcct ttaagtcctt ctgcttcaag gtaagaagac atagaaagat aaagatacgc
     8281 agaatataat tcatggttaa tctgctcatt aagtaatttt tccattttag ctgaaatcat
     8341 ttttatacct ccaaatttta aatgctctta taaaatataa taatattaaa tggaaaaata
     8401 ttcaataaaa gtgtttaaat attttacata tttaataaaa atttatacta ccattaaaaa
     8461 aattataact tacaatggct atagagccaa ttaatggtgc atttgctcca aaatttgaat
     8521 gccttacact tattttttta tatttagaaa aacttgctga agcagtcaat ttttttaata
     8581 atgtattttc tataaatgta ccaggtatat taaagtcata gcctattatt atagaactac
     8641 aatcaatcaa tgtaagtgta ttaactaatg catatgatac gtatgtacaa aattcatcaa
     8701 gcaaaaacat ggcaagacta tctttattat ttgcctcatc tattaacttg actaaagaaa
     8761 aatctttaca gttaattaat ggggagttag gataaatatg gctcaatgat tcaattcttt
     8821 taattagatt actgacactt gtataataat ctaaacatcc attgttacca caactgcaca
     8881 atggtccact aaaattaatt gatgtatgtc ctatttctcc actttgtcct aggttgccag
     8941 tatgaagttt attttctaga actagaccag ctccaatacc attcattatg tgtaggtata
     9001 tataatttga tatattttta cctaaaccat acattttttc tgctaatgca cccgaattag
     9061 catcatttat caaaaatatt ggcaaatcag acatttcttt tataaaatct actatattta
     9121 ggtttgatac atttccaaaa tctgggggat ttagtatggt accatttata tcatctactg
     9181 gacctattga agaaatacca attccaatta tatctctctt gttgattttc attattttag
     9241 taaacattct ttttattata tcttttaaaa catcatttga tagaaattta gggtaagttt
     9301 cagaaagttg ttttacaata ttacctttta aatctgaaat aattacctta catatatttc
     9361 ttcttattag gattccacaa atacaaggcg atatattaga aatatctaat aaaataggtt
     9421 tacgacctga tgaatgtgca tttaaagttg atgactctat ttccctaatc atattttcat
     9481 taataagttc agtaactaga tttgataaag tcattttagt aagtcctgta gacttagcta
     9541 aatcaactct ggacataggt gtatttgtag aaagtaattt caatattatt aacttatttt
     9601 taatttttac atctttgatg ttagaacctt gtttgttact catcataaat ctccttaaat
     9661 aaagttaagt gttttattta ttttcaagta tactatcacc ttataataga gtaacatttt
     9721 tttacttgaa aatatcatta attagaatat atcacattat atatcataaa acatataaaa
     9781 aaatattaga attaaattaa aagtgaaaag tagattgata tataaatagt aaatacttaa
     9841 ttaaaaaaag ataatgatag caataattat tgttaccaag aatctactac ttcaagtgtt
     9901 accttaggta tttaagttga tagtggtttg cacgtaataa acttgatgta atatatattc
     9961 ttaataatat cgataaaaga tttacttaaa aataatatag attatgtaaa catataatct
    10021 atttattgta tacaataaaa tttcattttg taaaacagtg aattaaatat gtaatataga
    10081 tatcgatttt tattacaaat tgttactaaa tataggtaca ttagaacaaa aatctagtaa
    10141 attgtaacta aaatgtaaat ttttccttta atctatacaa tattatggaa actttttgta
    10201 atttatgata taataacaaa tatataatta ttaaattata ataaatgact atagcgtaga
    10261 tgtaatacta tgttatagtc atttttgaat taaacttgat atatcaaggg ggaagaatat
    10321 gagaacaatt agaagagttt tagcattagg tttaacatta gctatatttc taataaatgt
    10381 acctaatgta gatgcattaa catcagatac aattaaaggt aacaatatat atgagacggc
    10441 tggattaata gcagataaaa agagttatga tacagcaatt atggtaaata tggataattc
    10501 tatagcagat ggtctttcag caagtggtct tgcaggtgct gttgatgctc caattttgct
    10561 tgcacaaaaa aataagatac caaatgaaac aaaacaaaga ttaaaaaatg ttaaaaagat
    10621 atacataata ggtaaagaat tatcaataag taagtcagta gaaactgaac taaaaaacac
    10681 aggggcacag gtaactagat tgggtggaga tgacagaata aagactagtt atagtgttgc
    10741 taaagaggta aatggtatca aaaaagttga tgaagtgata ttaactaatg catataaagg
    10801 agaagcagat acaataagtg ctgcacccgt atcagtaaga gatatagctc ctatagtact
    10861 tacagatgga aagagtgtgc ctttttcaac aagcaatgta aaaacatatg cagtaggtgg
    10921 gagtatatca atgagcacta gtttagttaa taaaactaat gctaaaagac ttggtggttc
    10981 agatagatat gatacaaata agaaagttat aaaagagttt tatccagatg catcagaatt
    11041 ttatttgagt gatggatatg atttagtaaa tgcacttaca ggttctacaa ttgctaagga
    11101 aaatccaatt gtattggtgt cagaaagtag tgataagtct atattagcag gagcagataa
    11161 gattactaga ttaggttcaa taagtgacag tgtgtataat aagtgtgttt ctgctgcaca
    11221 aaataatggc gattcgtcta caaaaggtga gtcacctatg aaaaatgaga caagcatatt
    11281 aggtcagcca acagctagtt tagaagcgtg cttaaaatgg gcaaaatcta aaaaagctaa
    11341 tgatttattt atagagttaa taccaatatt atatgatact gctgttcagg aaggtgttaa
    11401 tcctgttttg gcagtagctc aatctgcaaa agaaactggt ttttgtaatt ttggtggagt
    11461 attagatgca tcatttaaga atccttgtgg acttaaaact tctgtaggtg gttctgatac
    11521 tgataagaat gcccattcaa gatttgatac ttgggaagaa ggaatattag ctcaaattca
    11581 acatttatgc ttatatgcag gacaagatgg ttacccactt tcaaatccag tagaccctag
    11641 acatgaaaaa tctttatttg gtaaggctaa aacagttgaa agtttatcta ataattgggc
    11701 tggaggtcaa tatggacaag atttagtaag aatgatggga gaaattgagg caacaaaata
    11761 attgaagatt atcaaatatc caataaataa ggattgatta ttatgaaaaa gagtaatatt
    11821 tttagtatca tatttcctac agtcataatg gtgctaatgt ttttaacttt tgctacagat
    11881 atatttaata ttccagatat tcaatctaaa agtatttttg ttgtgggaat ggttttgatt
    11941 tttccaattt cttttttgat acaagggata atttgtgtat taaataagac taattggatt
    12001 ttatctctta ttgtttcttt agtaacttat atagtattaa tgtatatatt tttaaatgat
    12061 tctgcatata tatatgtttt actttataca gctttttata tgattggaca tataacagct
    12121 aaactctatt acaaaattaa gacttttaaa aattaaattg aagtatataa aagtaacatt
    12181 aggagaagta tccttaatga ttttctaaaa tcagaaatag gtgcttctct tttttgtaaa
    12241 aataaaagta tgtaaataaa atttaaccat tataattttc ttttaacaaa tgtatacaat
    12301 aaacttgttg tataattaag atagataaaa taaagggagg gataatatga atttaataga
    12361 taatcatatt catactaatt tttcaagtga tggaaaggat tccatggaag atacaataaa
    12421 aaaagctata agtattggtg ttagatattt gacttttaca gaccatttag aacatgatga
    12481 agatagagga ttttgcataa attataatga ctatgttcca gtatttaata agtttaaaga
    12541 aaaatataaa aaggacatag agttattact aggtgttgaa gtgggttaca gaaaacatct
    12601 aaaaaatgaa atagaagaaa taataaattc taatcctttt gattttgtat tgtgctcaac
    12661 tcatacaatt gacaatgtac ctgttccatc aaaggcgtat tttaaaggtc ttagcaaaga
    12721 agatgcgtat tataaatatt ttaatagcat acttgaaact aatcgtgaat ttggagatta
    12781 caatatatat ggtcatttgg attatatatc taggtatgga atatattcag ataatagagt
    12841 tatatacaat gactttaaag atgttattga tgaggtctta aagtcaataa tcaataatgg
    12901 gagtggtata gagctaaata cttctggtta tagatatgga ctaaatgcta tacatccaaa
    12961 tgaagatatt ctaaaaagat ataaagaact tggaggattt atagttactg taggttctga
    13021 ttctcatagg gtagaagata tatgcaaaga ttttgatgtg gcctatgata tgcttaagta
    13081 tcttgacttt aaatatgtat ctttatttaa agaaagagaa acctattttg taaatataga
    13141 aaaagttaaa tctaacaata tagcttagag tgtgttttga agtaaattaa tagatagtta
    13201 aaaagttaat agttatagta ttgtatcctc tagaaaaata ctagaggttt ttattttgct
    13261 aaaataacta aatagaatta aatttaaata gtatagctta ttttaaat
//
LOCUS       DAYASV010000102        13155 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYASV010000102 DAYASV010000000
VERSION     DAYASV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.103"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            251..817
                     /locus_tag="ACGI68_002803"
     CDS             251..817
                     /locus_tag="ACGI68_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0139230.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(1031..2017)
                     /locus_tag="ACGI68_002804"
     CDS             complement(1031..2017)
                     /locus_tag="ACGI68_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFH0139231.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGTRAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISEGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(2047..2742)
                     /gene="pxpB"
                     /locus_tag="ACGI68_002805"
     CDS             complement(2047..2742)
                     /gene="pxpB"
                     /locus_tag="ACGI68_002805"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFH0139232.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2762..3982)
                     /locus_tag="ACGI68_002806"
     CDS             complement(2762..3982)
                     /locus_tag="ACGI68_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFH0139233.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(4011..4802)
                     /gene="pxpA"
                     /locus_tag="ACGI68_002807"
     CDS             complement(4011..4802)
                     /gene="pxpA"
                     /locus_tag="ACGI68_002807"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFH0139234.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELEGIEVCPLENIEVCSSENIV"
     gene            5390..6019
                     /locus_tag="ACGI68_002808"
     CDS             5390..6019
                     /locus_tag="ACGI68_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HFH0139235.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6133..7647)
                     /gene="glpK"
                     /locus_tag="ACGI68_002809"
     CDS             complement(6133..7647)
                     /gene="glpK"
                     /locus_tag="ACGI68_002809"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFH0139236.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7891..8433
                     /locus_tag="ACGI68_002810"
     CDS             7891..8433
                     /locus_tag="ACGI68_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139237.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8438..10939
                     /locus_tag="ACGI68_002811"
     CDS             8438..10939
                     /locus_tag="ACGI68_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139238.1"
                     /translation="MFTLNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPAR
                     TIIQNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLI
                     CGLSSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIA
                     NILGSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGT
                     LMLICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILS
                     FEYFLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIA
                     APLSGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFT
                     MGTPLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDL
                     VGKPSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKS
                     LLNKVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGE
                     NKLNSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKE
                     LTTAYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVN
                     EQKESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRTMESQKKELQSKLNNSISQ
                     KKDMEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTL
                     QSTLNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            11360..11758
                     /locus_tag="ACGI68_002812"
     CDS             11360..11758
                     /locus_tag="ACGI68_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139239.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(11975..12184)
                     /locus_tag="ACGI68_002813"
     CDS             complement(11975..12184)
                     /locus_tag="ACGI68_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139240.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(12216..12425)
                     /locus_tag="ACGI68_002814"
     CDS             complement(12216..12425)
                     /locus_tag="ACGI68_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0139241.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            12724..13143
                     /locus_tag="ACGI68_002815"
     CDS             12724..13143
                     /locus_tag="ACGI68_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139242.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
BASE COUNT         4649 a         2098 c         1627 g         4781 t
ORIGIN      
        1 atttactctt ttccaaaata taatgtgata atcttaattc aattaatact cttctattaa
       61 atttttttat tattattttc tttgtattaa tttaacaata attcattttc atagactttt
      121 atttggattt aattaaattt taattgtcaa aatctactat gtattcttat tgccactact
      181 tctatgttgt gctataatta ctttgtttta agcgaatatt ttatattaat tttattattt
      241 ggaggttctt atgctaggtg aaaagatgag aaatataaga aaaagcaaga aaaaaacatt
      301 aagtgatgtt tccaaattga cagatttatc tataagttat atctctcaaa tagaaagaga
      361 tgctattgaa ccatccttgt cttcattgag aaaaatcgct gaagttttag acactccttt
      421 atatatgttt atggatgaca ataatactga tgatttagtc attagaaaag aagatagagt
      481 tatgatgaaa tttccaaaga gtgaaatgtt ttacgagata gtatctccaa tgcctacaac
      541 tgagtttaca ccttcaattt tatttgtaga gtttgaactt aaaccagaaa gtgaggatgc
      601 caaagattat atatatcatg catcagaaga aattatggtg gtaacgagtg gtgtggttga
      661 tatttgtatg ggtgaaaagg tagttagatt aaatccaggt gactcaacat ttataaaagc
      721 taatgtacca cataaaatca tcaatccaag taaagataca atagctcgtg gatatggagt
      781 tatatcacct ccaatttggc caataaaatc taaataaact caatatgaca cacctacttt
      841 ttgtagataa tataatagat ttaaatattg cttttatttt aaaattgaag tatttattta
      901 aatttaaaat atttactcaa actaaaaaaa tagctatttg actaatctaa accatcttaa
      961 ttatggtaaa tagtaaatag ctatttgtaa gttatattat aaactataat aaaaattgta
     1021 tttgttaaaa ttaaactgct atatcattta atacttttgc ctttacaact cttatatttt
     1081 tcatactctt gtagatatct tgaatcttat cttcttgctc tctcaatagt ctatgtgctt
     1141 catatatatc tacaagttca aattttacta catcatttgg ctttgcttgt gctaacttat
     1201 acaaatctac acttataacg ttccctatct tagtatagcc accactagtc tgtctatctg
     1261 ataacattat aataggtttt ccatgaccag gtacttgtat agctccataa gaaataccct
     1321 cagatataat atctgctcca ttaaggtgct ttatactctc tccatctagt ctacaaccca
     1381 ttctatctat ttcattcgta actttgtatt tatttgataa aaatttctct acttcaccgt
     1441 tagaaaaagc atcaaattga ggacctttta caattcttag tacgatttct gaactataag
     1501 ttggaatata ttttgaactt aattttttat ttattgtaaa atctttttcc tgaataaaag
     1561 tatttatata atcgcctttt ttaagtgctc taccttcaaa tccaccaatt ttagctcttg
     1621 tataagttga cttacttccc attgcaagtg gaacatctat tccaccagct attgacacat
     1681 aggctctagt acctgatttc acacctctaa atgataatac atcacctgga tttatttgat
     1741 atgacctcca gttttgaatt tcttttccat ttattttagc ccctaaatct cccccagtta
     1801 ctgctatttg cgtatattca tcaaaggtaa ttgtaggacc taacattaaa acttctaaag
     1861 aagcctcatc ttcagaattt cctactaata tatttcctac tctatgtgca tactcatcca
     1921 ttgctccaca tccagataca ccatattgac catagccaac tctacctctg tcttgtatta
     1981 atgtttggaa acctcctaat tcaactttga atcccatata taccactccc tgtactataa
     2041 cgatatttaa atacttaagt tatttttttc gatttctata aattcatcta atgttattgg
     2101 aacaaatttt ataaagtctc cagctctaag caaaatctgt tcttctctat tttcatcata
     2161 taattttaaa ggtgtcctac caattatttg ccatccacca ggactatcaa taggatatac
     2221 accagtctgg gaacctgcta ttccaacact cccaccataa attttagttc ttggcacttc
     2281 taatctaggt gtagctattc tttcatccat accacctaga tatggaaatc caggagtaaa
     2341 ccctagcata tatatcaagt actctctgct tgtgtgtatt tttataactt catctacagt
     2401 gagattattg tagcttgcaa cgtttttaat atctatacca aaaacctcat cataacatac
     2461 tggaatctca tgtattttag gttttggtaa tactatactt tccaaattat cttctatttt
     2521 ttttaaattc tcaatcaaac tatcaaaatc tatttctaat ggattataac ttatcataag
     2581 tgacctatat gttggtacca tttctgttac aataccttta agattcgata tttctattgc
     2641 tctcataaat gaaattactt ttttgtttat atcttctgat atctcatttc caaattcagc
     2701 aaccactgcc ttatcccctg atatcaagta tctagtttcc ataaatgtca cctcttctaa
     2761 attaagcaaa taaagcacct aggctgctaa gagaagttat tcctgtatat gcagatatta
     2821 aaactactat taatccaaat attaaaagcc atgtaggatg cttgtaatct ccaacaatat
     2881 cttttctttt agatgctata agcataattc ctaatgtgat tggaagtatc aatccattta
     2941 aagctcctgc taaaattaat aaagtagctg gttttcctat aaatgccatt ataagtgttg
     3001 atattgctat aaatgctatt ataaaatatt tttcatattt atctataaaa ggatttagtg
     3061 tctttaagaa agaaactgat gtataagcag ctcctataac tgatgtaatt gcagcagacc
     3121 ataatacaag tccaaagaat ttatatccta ttgctcctgc actaaattta aaagctgaag
     3181 ctgctggatt ttctgggtca agttgaagac cttttgaaac cactgccagt attgctaaaa
     3241 ataaaagaac tctcatcatt gtggcaacta atatacccaa aagagaagat ttagttattt
     3301 cttttatatt ttcttctcct gttattccac cgtctattaa tctatgtcct ccagcaaaag
     3361 ttatataacc tccaacactt cctccaagta aagttattat tgggaatatc agtccttttg
     3421 gattttctgg ttttaccatt cttgatactg cctctccaac aggaggatga tttgatattg
     3481 ctacatatcc aactataatt atcattccta atgctaaaaa tttagttatc ttatcgacta
     3541 gagaatttga attttttgac ataaatacaa atatagctat taatccactt aatacagttc
     3601 ctaatgtcat attcatatta aacattacat tcataccaag tgcagctcca ccaacatttc
     3661 ctatattaaa agctaatcct ccaagagcta ctaaaaaagc tactaaatag cctaatccag
     3721 gtattatttt atttgctata tcctgacctc taagtcctga tacaccaatt actctccata
     3781 catttacttg agcccctata aaaaggattg ttgtaattaa aattacaaat gaaaaattcg
     3841 gtccaaaatc ttgggtaaat tgtgcagtct gagttaaaaa ccctggtcca attgctgatg
     3901 ttgccattat aaatgcagcc cctataagtg ctccgacatc ttttttattt ttagttttat
     3961 ctgtaatatt ttttgtactc atattttatc tccccctcat gttatcagtc ttatactatg
     4021 ttctctgacg aacaaacttc tatgttttct aatggacaaa cttctatacc ttctaattca
     4081 aaacgctttc ttatttcttt gacaaactct attgcttttg gattgtcccc atgtacacat
     4141 atagaatctg ctttgatatg tataacttca cctgttaaag tttctacagt tccttctttt
     4201 accatcctaa caaccctatc tattgcatgt tttgtatcat gtataactgc tccttctaca
     4261 tttcttggaa ccaaaaaacc attattatca taagctctat ctgcaaatac ttcatttgca
     4321 aacctaagtc caatttcttt agcagaattt atcatttcac tattatatag ccccaacagt
     4381 ataatatctt tatcgacttc atatatagct tcacaaatcc ccatagctaa ctctttatct
     4441 ttagctgcca tattgtataa tgctccatgt ggttttacat gttgtatatt acatccattt
     4501 gaacttgcaa atgccattaa agcacccagt tgatattttg tataatcttt agcttcctgc
     4561 tttgttattt tcatctcacg tcttccaaat cctattaaat ctaggaaacc tggatgtgcg
     4621 cctattttca ctccattttt tttagctagc tgtactgttt tttccatatg actagggtca
     4681 cctgcatgaa acccacaagc tatattggct gatgaaatat attttaaaac ttcttcatct
     4741 aaaccaatct tatacgttcc aaaactttct cctaaatcac tatttaaatc tactttatac
     4801 aatttatgac ccctcctcgc taacattttg atagaataag tagttttaat ttttaaattt
     4861 tgtttatttt atatattgca tgaaatattt tatcacttgt attcaatttt atttttgcaa
     4921 ctattctatt gcatttaatt aatataattt taccatttaa ggaatatttt tgcaattatt
     4981 ttaattgaac attcatttgt atttcttttt caaaaaatct ttttaccatt ttttagttaa
     5041 taattaatta ttttcactta aaataagggc tttaaccttc atttttaaag tatttttcat
     5101 ttttaatcac tttatatgca agacagaatc atttttcttc attcatttgt ttctttcatc
     5161 tatataatta tcattctttg taagtaaaat atctttatat ttttataatt aattctttat
     5221 atagttttaa gtataataaa atcatgtata ctaaatgcat gttaaaatat tattttaaaa
     5281 catgcacaat atttgtacat attgtagaca tataaaccta taaattatac aatagtattg
     5341 ttaagattat atattttttt aacataaatt ttgtattggg ggaaaaacaa tggataaaaa
     5401 ctatacgatg ccaatatacc aaaaaatagc gttggatatt gcaaataaaa tatacacggg
     5461 tgaaatacaa gaggattcag tactatttgg acgctctgtt ttggctggta aatacaatgt
     5521 atctccagaa acgataagaa gagctgtcaa aattttagag gacattggcg ttgttaaaag
     5581 cataaagggt aaaggtgtta ttgtactatc tccagacaaa gcttcttctt ttataaaaaa
     5641 atatagagat attacaaata tatcatcata taaatcaaca ctttataatt taatagatac
     5701 taaatctaat ttagaaaatg aaatattaga tactataaat aaaattttag actattctaa
     5761 cagacttgaa ataatcaatc ctttagtgcc cgttcagttc accataaatt ctaattgcaa
     5821 atatattggt caaacagctg cacaaactaa attttggcaa aatacaggtg ctacaatagt
     5881 tgcaattaaa agaggtgaag aacttataat atctcctgga ccatatattg aatttttaga
     5941 aggagacatt ctcttagtag ttggtgacca acacatttac aattctatac caatgttttt
     6001 atatgaaaac gaaaaataaa ttatataatt tagtgtgatt atataaaaat gcaagattaa
     6061 caacaaaaaa agagcttcat attttacata aagctctttt taaattcaaa taaatttaac
     6121 tttgattaat ttttaaatat ccaattcatc ttctttttcc cagcttaaag ctctcgatac
     6181 agcttttttc catcctttat ataatttaca tctcttctct tcactcatat ttggattaaa
     6241 ctctttttct actgaccact ttgaagtaat ttcatttctt ccaatataga atccaacaga
     6301 caaccctgct aaataagcag ctcctaaagc tgtagtttct actattttgg gtctatctat
     6361 atctacattt aatatatctg attgaaattg cattaaaaaa ttattattac ttgctccacc
     6421 atcaactttt aatgatttta gttttaattt tgaatcgttt tgcattgctt ctaaaacatc
     6481 ctttgtctga taagcaattg attccaatgt ggctcttact atatgttctt tttttgctcc
     6541 tcttgtaagt cctaaaattg aacccctagc atacatatcc caatatggag ctccaagacc
     6601 tgtaaatgct ggcactacat atacaccatt agtatcctct accctatttg catacatttc
     6661 actatctttt gcagattcaa ttatctttaa ttcatctcta agccattgaa cagatgctcc
     6721 tccaataaat atacttcctt ctaatgcata tgttactttt ccatcaactc cccaagcaat
     6781 tgttgtaagt aacccatgct tagatttaac tatgttactg cctgtattca taagcaagaa
     6841 acaacccgtt ccataagtat tttttgcagt accttcttca acacaatttt gaccaaataa
     6901 tgctgcttgt tggtctccag cacatcccgc aataggtatt ttagctcctg ataacatgcg
     6961 ctcatctgta tgaccataaa catagctaga aggttttact aatggcaaca tgcttattgg
     7021 tatgtccaat tctcgtaata tatcctcatc ccattctaaa tcatttatat tgtacatcat
     7081 tgttctagat gcatttgagt aatctgttac atgtactttt ccacgagtta aattccatat
     7141 taaccatgta tctatagttc caaataataa ctctccttgt tctgcttttt ccctagctcc
     7201 ttctacatta tctaatatcc attttacttt tgttgctgaa aagtatgcat ctattaaaag
     7261 acctgttttt tcttttattg attcttccaa tcctttattt ttaagttcat cacatatttc
     7321 tgacgttctc ctacactgcc atactattgc gttataaata ggtttaccag tatacctact
     7381 ccaaacaacc gtagtctctc tttgattagt tatccctatt gaagcaattt cttctggtct
     7441 tatacctgct gtttctataa cttctctcat taccccactt tgtgaccccc atatttccat
     7501 agggtcatgc tctacccatc cagcttttgg ataaatttga ttgaactctt tttgtgcaat
     7561 tttcactatt tcgccttttt tattaaatag tatagctctt gaactggttg ttccttggtc
     7621 taatgccatt acatacttct tttccatcac catattctcc ttatcatata atatttaatt
     7681 tattaaaaag aaaaatttaa tatcacatat ttcaataaat aatcatactt aagtacaaat
     7741 ttaatataat agatatttag taagaagaca cttattttct atcatatcta tataataata
     7801 ttatacatta tagcttacat attgattaat aaattttaaa ggagtataat aaacagtgtt
     7861 cataataatt ttaataaaag aggtggtaat atgccaaaga ttttagaaaa tgtaaaagaa
     7921 gatatattaa aagtgtctcg ggatatgatt ttagaagaag attactcgaa tattagcata
     7981 aggaaaatag ctcaaaggtg tggtataagt gcaggtacag tgtataatta ctttaattca
     8041 aaacaagaaa taatagaata tatcacaaaa tctgaatggg atttattgat aaggcgaatt
     8101 gaatactcca ataaaaacac tgaagattac ataaaaaaac taaacattat ttttactgaa
     8161 ataagaaatt ttataaacaa agttcataat atccaataca atgacttttt aaacactttt
     8221 gaaacagaga gattttttga aatgaaaaaa cacaaagacg actttcatga acagctttca
     8281 aataaagtat atgaagcttt agaaaaagaa agttttttta ataatgacaa attagtatgc
     8341 aatatcataa taaaaatgtt tttttcatat tcaacatatc ttcatataga atttgaagat
     8401 ttaaaaccct atattgaaaa attaatacat taaggtggtg tttaccttga atcataatac
     8461 tagtattaag aacaaggaca gtaatccaaa aatattttta gtacttacgc tttatctatt
     8521 aggtatattt atgggagcta ttgatactgg aatagtatca ccagcaagaa cgattataca
     8581 aaatagcctt ggagtaaatg aaaaaacggg aatttggatg attaccatat acacattaac
     8641 atacgcttct gtaattccta tctctggaaa attagcagat aaaataggaa gaaaatatgt
     8701 gtatctagtt agtatatttc tatttggtag tggttcatta atatgtggat tatcatcact
     8761 attttccaat ttctatattt tattaattgg tcgtgttatt caagcaattg gtggtggtgg
     8821 aatcatgcca attgctacag cagaatttgg aactaccttc ccagaaaata aaagaggcat
     8881 ggctttaggt ttagttgggg caacatatgg tattgccaat attttaggtt caagcattgg
     8941 tagcacaatt ctaagtatct ttggaactca aaattggaag tggctatttt ttgtaaatct
     9001 tccaatttgt ttaatcatac ttattggtgg agttttctgt attaaaaaca ataagtctga
     9061 atctaaagaa aaaatagata agcttggtac attgatgttg atatgtataa tcgtatcact
     9121 attatatggt cttatgaata tagacttttt taattttaaa aattctatac aagatatatc
     9181 agtttatcca tacttattga catttattgt acttatacct attttcatat ttattgaaaa
     9241 tagagctaaa gaccctattt taagctttga atatttttta aatccgagaa tattgatagt
     9301 acttatttta tctttaattg ttggtattgg aatgatgggt atggtattcg ttccacagta
     9361 tgctgaaaat gcattaaaga taaatgctgg ttctggggga tattttgtag ctatattggg
     9421 attatttgca ggtattgctg ctcctttaag cggaagactt attgacaagt ttggggcaaa
     9481 aaaaatacta ttactaggat tttctgtatc tatgataggc tctctttacc tagttttaat
     9541 tgctctaaaa accaatactg tatttagtgt atgtgtaagt ttaatattta tgggattagg
     9601 aatgggattt actatgggaa ctcctctaaa ctatatgatg ttatcaaata caagattaga
     9661 agaatctaat tcagcacttg caacattatc cttgatacgc tcaattggta cttcaatttc
     9721 tccagctata atgattggat ttatagcaca tgcaggatta tctgtgcaag ataatattat
     9781 ggacttagtt ggaaagccct caactcctaa aatagttcaa cttgaagaac ttgaaaacat
     9841 gatagatgac ttaaaatcag accctgaaat ggcaaaaaat ttaaaaaatg tctctatccc
     9901 aaatatgaat gatacttcaa atataaatat ggacatgaca ggtggcaaac ttccaaaatc
     9961 cttacttaat aaagttcagt cagcagatac aaccaacatt accaatataa caaaagaaat
    10021 atcaacaaca atgtttgatg aaaaagttcc atctataatt actaaaatac agaaaaatgt
    10081 acaaaaagga atagatggaa cacaaagtgg aataaacggt attgaagatg gtgaaaataa
    10141 actaaatagt ggtattaaag gagttcaaac tggtattgac aatatggtaa aagctagagc
    10201 tggtattaat caaggaatag atggtgtaaa aaaaggtatt gctggtgttg ataaaggtat
    10261 aaaaggtatg gaacaaggtc ttgaaaaaca agatgaggcc atcaaggaac ttacaactgc
    10321 ttataataaa atacctacta cagaaaaaac tcctactgat aacgaaaata actcaaatga
    10381 acaaaaccag agtaatacca gtaattctga atcagacgac actaagaatg attctacaga
    10441 aaataagaat aacaattcca gcgagaataa accaaatgta aatgaacaaa aagaaagttt
    10501 aaatgctcaa atacagcaac taaaaaagac aagagatgac ttaaatacaa agttacaaaa
    10561 aaataaaact caaaagaacg aattaagtaa aaaactaaga acaatggaat ctcaaaagaa
    10621 agagctacaa agcaagttaa ataattctat aagccaaaag aaagacatgg aaaaaagcct
    10681 tgatacaatg caacagcaaa aaaaatcact ccaagcagtt ttagcaaaga cacaagaggt
    10741 aaaaactgaa ataccaaagg cttttgataa atcaaaattg gattatataa attccataga
    10801 aaataataga acaaaaatag aaaacacact acaatctact ctaaactcag gatttaaaca
    10861 gatgtatata acagtattct gtgtaaatct actagcattt ataatattat tgttctataa
    10921 agaaaataaa actagataaa agagtgttat agtattatat tgtaaattta tcttattctt
    10981 aaatatttta ttctaaagaa taaattcata aaatttttgt aattgattaa tataaattac
    11041 aatataatac tatttttctc ttaatctttt tctcttttac agacaatcaa aaagcaatct
    11101 tagtttatcg aaataaattt atacaatcta ttcatacacc cctttacatt tatataacaa
    11161 ttaaacctat tgttatatat acaaatcaaa ataaaccata tattttcaac tataatatgt
    11221 taaaatcata ttatgagcat attaatttaa gcaaaatgtc atttagactt taatttagta
    11281 gttttatatt ataaatttat atatttttaa ttttaaatgt catttagaca ttactcataa
    11341 aaagaaagga gtgtattttg tggatacttt aggaaaaaga attgcttatt tgagaaactc
    11401 caaaaaactt actcagcgca aacttatgga tattttaaaa tttgaaaact taggcaagta
    11461 tgaaacagga gatagaaaac ctaattgtga tatactaatg tcaatagctg attattttaa
    11521 tgttacgact gactggcttc tctatggtaa agagaaggta aatgtcaata gtagtgtaaa
    11581 agaagataaa gaagattact tacatgttac taatgatgaa atgacgatat taaatctata
    11641 cagacaacta aacgaacgtg ataaaataaa aatagaagga atacttgaac ttaagatatc
    11701 tgaatataaa gacttaaaaa agcattcctc taataataat gaagataaaa tggtttaaac
    11761 atacataatt tgatacgatt ttacatcatc tattaacctt ctttttttgt agtatattac
    11821 taaaatatgg atattggatt atttaaaaac aagtatagtc taatttcaag cacatttata
    11881 atatttagat tatcttatcc atattttagt ttaagtactt tgaaaaatat aatacatctc
    11941 tatgtataac ttatattcct aagaaatgca ttactcatgt ttttaattct tccaacatct
    12001 ttctatacac aattgtacat ccttcataat tcccctcttt aagaaggatt gaacatgtac
    12061 tagaatattt agttaaagta tttaatcttt ccatatttaa gctttttata ttactgagtg
    12121 ctgtaataaa atcatcaatt gctctacaac actctataac cccatctgaa tccaatttac
    12181 acacaagaat attcacctac tttatatact atttattaat catcttctga atattctata
    12241 ttaagtaatt gataaattga tgatttatat tttttacctg ttcttcttcc atttaaaata
    12301 gctgacaaat agttttcatt aacacctatt ttcttcgcta attccctttg agtcatattt
    12361 aattcaataa gtctttgctt gacttgcact cccaaaaaag gtatccattt ttttcgagca
    12421 tccatttatc aacttccttt ctaagtgatt tatatttact tttatattat tttagcttac
    12481 acaccccctc tatttagtat atatttttaa tatgtacaca tattaagtat ataaatttat
    12541 ttaatactaa ataagatttt ttgattactt tgtgctaaaa ttaggatgta aattcgtatc
    12601 attaagttaa ttataaagtc attttgacct taagtcaatg ctttttatgt cattttgacc
    12661 ttaaatttga aactatttac atcttaaaaa gtctttttga cattaattga atggagagtg
    12721 tttatggata ctttaggaga aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa
    12781 tatgaactag aagaaatgtt gggctgtgac aacttaagta aatttgagag aaacattaga
    12841 aaaccaaatt atgaaatatt aaaatctata gctgagatat ttaatgtttc agtagactgg
    12901 ctattaaatg gagataatct atcacataaa tcagatttaa tttgcgattc ctcctcaaac
    12961 tatccactta atagcataaa ctctaatgaa ataaagcttt taaacaattt tagaaagtta
    13021 aatgattatg ataaagccaa aatagaaggt atgattgaat taaaacttca tgaatatgaa
    13081 aaagagaagg atttaggaaa aattgagtat aacaaaaata aagatgagaa aatagataaa
    13141 taatttttat gttgc
//
LOCUS       DAYASV010000103        13129 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYASV010000103 DAYASV010000000
VERSION     DAYASV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.104"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     misc_binding    28..259
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            367..1848
                     /gene="thrC"
                     /locus_tag="ACGI68_002816"
     CDS             367..1848
                     /gene="thrC"
                     /locus_tag="ACGI68_002816"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFH0139243.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            1858..2751
                     /gene="thrB"
                     /locus_tag="ACGI68_002817"
     CDS             1858..2751
                     /gene="thrB"
                     /locus_tag="ACGI68_002817"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFH0139244.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            2881..3936
                     /locus_tag="ACGI68_002818"
     CDS             2881..3936
                     /locus_tag="ACGI68_002818"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFH0139245.1"
                     /translation="MKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVISG
                     VQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEAVK
                     AGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIVVE
                     GPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSSSD
                     VKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAIRN
                     KFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAYKI
                     DKLSTVEDVINELISEL"
     gene            complement(4091..4603)
                     /locus_tag="ACGI68_002819"
     CDS             complement(4091..4603)
                     /locus_tag="ACGI68_002819"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFH0139246.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKESKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            5123..6058
                     /locus_tag="ACGI68_002820"
     CDS             5123..6058
                     /locus_tag="ACGI68_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFH0139247.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(6150..8021)
                     /locus_tag="ACGI68_002821"
     CDS             complement(6150..8021)
                     /locus_tag="ACGI68_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0139248.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            8211..9137
                     /locus_tag="ACGI68_002822"
     CDS             8211..9137
                     /locus_tag="ACGI68_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFH0139249.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            9276..9422
                     /locus_tag="ACGI68_002823"
     CDS             9276..9422
                     /locus_tag="ACGI68_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139250.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(9527..10051)
                     /locus_tag="ACGI68_002824"
     CDS             complement(9527..10051)
                     /locus_tag="ACGI68_002824"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139251.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(10104..10328)
                     /locus_tag="ACGI68_002825"
     CDS             complement(10104..10328)
                     /locus_tag="ACGI68_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139252.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            10525..11649
                     /locus_tag="ACGI68_002826"
     CDS             10525..11649
                     /locus_tag="ACGI68_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HFH0139253.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISTFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSDSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(12123..12947)
                     /locus_tag="ACGI68_002827"
     CDS             complement(12123..12947)
                     /locus_tag="ACGI68_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5780 domain-containing protein"
                     /protein_id="HFH0139254.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         4614 a         1899 c         1643 g         4973 t
ORIGIN      
        1 tagaataatt aataatatta agaatattga aaggcattga ttaggaagag taaatctgaa
       61 tcgattacca gagaaggaaa ctactagctg aaatgtttcc ctaattagaa ggattgaaaa
      121 ctacctagga gcttctatcc gaagttatct taatgatttt agtaggaata gacgttttta
      181 ttacttgcgt taaagtatca agtggatttt ttaaaatcaa tttgggtgga accgcgggaa
      241 acagattctc gtcccttttt tagggatgag ggtctttttt tattacctaa attcagtttt
      301 taaaatattc tgtatctaat ttaattagct aattaaaaat ttaaaatata tggaggatta
      361 taaaaaatgt attattcaag tacgagaggc accgaagaaa aagtaactgc atcacaagca
      421 ataataaaag gaatatcaaa tgatggtggt ctttatgttc caagtgaatt tcccaatgta
      481 aaaaatgaac ttataaattt agtaaattta acgtattctc agatagcatt ttttgtactt
      541 agtaaatttt tatgtgattt tactgaagat gaaattaaaa attgtataga aaatgcatat
      601 gatgaaaagt ttgattgtag tagtatagct ccattaaata aagtaaatga tacttatttc
      661 ttagaactat atcacggtcc aactttagca tttaaagata tggcacttac tataatgcct
      721 catcttctta aaacatctat aaagaaagat aatttagaga aagatgttgt gattcttact
      781 gctacatctg gagatacagg taaagctgca cttgagggat ttaaagatat agataaaata
      841 aagataattg tattcttccc agaagatggt gtaagcccag tacaaaaact tcaaatgaaa
      901 actcaaactg gaaaaaatac atatgtagtt ggtataaaag gaaattttga tgatgcacaa
      961 tcaggtgtaa agaagatttt ttctgataaa gaatttaatc aaaaactatt ggataataat
     1021 tatatattat cttctgcaaa ttcaattaat ataggaagac ttcttccaca agttgcatac
     1081 tatttttatt catatatgaa attagttagc aatagtgata taaatttaaa tgataaaata
     1141 aattttgttg tcccaactgg taattttggt aatattctag ctggatatta tgctaaacaa
     1201 atgggtcttc caataaacaa attaatatgt gcatcaaatg ataataatgt tctttatgat
     1261 ttctttaaaa ctggtgtata tgataaaaat agaacattga aattaactac ttctccttct
     1321 atggatatat taatttcaag taatttagaa cgattactgt ttgaaatatc aaatagaaat
     1381 actgatattg taaataagct tttatcagac cttagtgata aaggagtcta caaaataagt
     1441 gatgatatgg aaaaaaattt agcctcattc tatggagaat attcaaatga agaagaagtt
     1501 aaaagcacga taaacaatgt gtttaaaaat tataattatt tgatagatac tcatactgct
     1561 gttgcataca attgttatga aaaatataaa aaagaaactt ctgataacac aaaaactgtt
     1621 attgtaagta ctgcaagtcc attcaagttt tctgaagatg ttctaaagtc tatagattgt
     1681 gattttaaag accttgatga ctttgctata attgatagat tatcaagtat ttctaaaata
     1741 gatattccaa aacctattaa aaatcttaaa aatgcagaaa tactacataa agatatttat
     1801 gaaaaagatg aattaaaact agctataaaa aaattcttaa aggtataggt gattattatg
     1861 ttagagatta tagttcctgc aacaagtgct aatataggtc caggatttga ttgtcttgga
     1921 attgcgttaa atatatacaa taaattttat gttgaagaaa ttgaaagtgg tcttgaaata
     1981 gaaggttgtg aagatgctta caaaaacgaa aacaacttag tttatacatc tatgaagtac
     2041 ttttttgata gagttaaacc agaaaaaata cctgctggta tcaaaataaa aatccaaagt
     2101 gaagtaccta tatgtagagg tcttggcagt agtgcctctt gtatagtagc tggagttata
     2161 gctgcaaatg ctctatcagg tgctaattta gataaaaatc aattactaaa tatagcttca
     2221 gaaatagaag gtcacccaga taatgttgct cctgctattt taggaaatat gattgtatct
     2281 gttactgata atgagaatat acattatgat attataaaaa ttcctgaaga acttaaattt
     2341 tgtgcaatga tacctaattt taagttatca acagaaaaag ctagaggtgt tttaccaaag
     2401 gaaataccat attctgatgg ggtattcaat gttagtagag ttgctttact aatatctgcc
     2461 ttactaaaca aaaactttga cctacttaaa gtagcttgtc aagacaaatt gcatcaagat
     2521 tatagaggaa ctctaattga gaactacaat gatattgttg aaaaatctga acaattaaat
     2581 agtattggag tatttttaag tggagctgga cctactataa tgtctttaat aaaagaaaat
     2641 gatgatagtt ttgtggataa tatgaaaaat tacctacaaa aactaaaatc tgattgggaa
     2701 ataaaagagt tatgttgcga ttctaatgga gctgttttaa atataatata attatcgtct
     2761 tctatacaaa atataactac tcttttatta taatttgtag tataataatt aggactaggt
     2821 aataatatta agtattataa tctagttctt taaacatgtt tacattgagg agggattatc
     2881 ataaaagagt taataattag agatttagtt gcaaaagttc ctataattca aggtggaatg
     2941 ggcgttggta tatctaggtc atcactagct ggtgcagttg ctaagcttgg tggtgttggt
     3001 gtaatttctg gagttcaaat tggatatgat gaggaagatt ttgaaactaa tactataaat
     3061 gcaaatttaa gagctataaa aaaacatatt tctaaggcca aagaaatctc aaatggtggt
     3121 attattggta ttaattttat ggtagctatg aaagaatatg aaacatacgt aaaggaagct
     3181 gttaaagctg gtgtagacct tataatatct ggagctggat tacctaataa gctaccatca
     3241 ttagtaaagg gaagtaatgt aaaaatagca cccatagttt caactgcaaa agctgctaat
     3301 gtaattttaa aaatgtggga tagaaaagag aaaactactg ctgacttaat agttgttgaa
     3361 ggtccaaaag ctggtggtca ccttggatac tctaacgagg aacttgataa catagattct
     3421 atagactatg ataaggaatt tgttgaaatt ttaaaggttg caaatactta tggagaaaaa
     3481 tttggtagaa acataccagt agttgctgct ggtggtataa cttctagtag tgatgttaaa
     3541 aaatatatag atatgggtgc aagtggtgta caagttggta caagatttgt tgctacttac
     3601 gaatgtgatg cccatgagaa ctttaaaatg gcatacataa atgctagtga agaagatgtt
     3661 catatagtta aaagtcctgt aggtcttcct ggacgcgcca taagaaacaa atttatagaa
     3721 gaagttaaaa taaatcgtcc agaaataaag aagtgctata attgtttgat tccttgtaat
     3781 ccaaaagaaa ctccttactg tatttctcaa gctctaatca atgcagttaa aggagatgtt
     3841 gaaaattcac ttttattttg tggaaatgat gcctataaaa ttgataaatt aagtactgtt
     3901 gaagatgtta tcaatgaatt gatttctgaa ttataattta aagaaaacta aattaaaatt
     3961 ttatcaagct aaggagttta aatacgttat ttattaatct ttataaacat atttatgtcc
     4021 ttagcttttt tgtgtcaatt tccttttatg gaaattttaa tttaattaga gatagtatta
     4081 acctttataa ctaatcttct tttacaagta cattggttat ttcagctata tataaagtat
     4141 gataatcttt ttgtggatac catcttccat ctataccttc agcaataaaa cattgaggtt
     4201 caagtttctg actatacatt tttttgcata taatagccat tttagattct ttaaaataag
     4261 gtgtatcatt taagtgttct atattaaatc caatctgtga tatcttatcc tcttctctac
     4321 cagatacctt tccacaatat gaaagctctt tacgaaaatc ttcatcaaaa aatgtaagag
     4381 aaaatttatc tgtattatct ataaattcct tagtataacg ctgtggtctt attactacaa
     4441 aagcaacatc ttttccccac ataactccaa gccctcccca gctagcagtc attgtattca
     4501 ctttaccttc attttctgct gttataagtg tccaatcttt acctatcaat ccaaatggat
     4561 tatttttaat ctcttctgcc aaaatctctt taaaatcact catatctcta ataacctctc
     4621 attctcttat atttatattc cttttatttt cgtgataaat tataaatgaa gtctagtttt
     4681 gaaaataaaa tactttttta aatatattat tgcaaataag taaatatata tatactctct
     4741 tattttttaa tatatttaat aatttcactc agaatatata tattctttac tcaaattaaa
     4801 tataacttat atactttatg tgtaaattat ggtaaaaata ttatttaatc aaatcaaaac
     4861 ttgatttata tacctggaaa aggtattata ttaaagagtg cttaaaatac gcatttcata
     4921 aatagctata tataaaataa taaataagga gagtgatggg caatggatac tagtccgttg
     4981 cggagagaat tttttacatt ttcatcactt aataatatct aaaatcaaaa ctaatacata
     5041 tattcagtta tataaattag ttaattaata gatactatta aatgatgaga tttatataaa
     5101 actgaataaa ggatgtgttg ccatgataag atattacaaa actatagatt caaaattaga
     5161 gaaacttagt ttttttgaag atggctgttg gataaatctt gttgaaccta atcatagtga
     5221 aatcaacgaa atttctaatt tattaaatat agatgtagaa agcatagaat ctgcactaga
     5281 tgaagaagaa cgctcaagaa tagatgttga agacaatcat actcttatac ttatagatat
     5341 accagtagac gaaagtgact ctaattcttc tcattatact acaataccct taggtattat
     5401 actaactgaa gaagctattg taacagtttg tgatgcacaa actaagattt taaatgactt
     5461 tatagttggt catattaaag atttttttac gtttaaaaag actcgctttt tgcttcaaat
     5521 tcttcacaaa aatgcagctt attacttgca ttacttaaga aaaataaata aaatgacaat
     5581 cataatagag cgagaaattt acaaatcaat gaaaaacaaa gaattggttc aattattgga
     5641 attagaaaaa tctttggtat acttctctac ctcactaaaa tctaatgaac ttgttttaaa
     5701 caaaatggtt agaactgctg gtataaagaa gtatccagat gatgaagacc tattagaaga
     5761 tgttatagtt gaaaatagac aggctcttga tatggctaaa atatatggag atatattgag
     5821 tagaattatg gatgctttca gtgcaattat aagtaataat cagaataatg ttatgcaaat
     5881 tttaacagta gttacactaa ttttctcaat acctacaata atatcaggat tctttggtat
     5941 gaatgtcatc aacatgccat tttctaataa cccaaacgga ttttggataa tacttcttat
     6001 tacctctata atatgtatag tgattacatt tttcatgtca agaaataagc tattataaaa
     6061 tattaataaa aaggccatta gaattaattg atttacattt atcctaatgg cttttatata
     6121 tttaaatcta tataatctta taatactttt taactatcat gttcttcatc tataatcatt
     6181 tcagtttcaa gcccttctag cattgaatct aaactttctt ttatatttag tatataaatt
     6241 ggtacatact cttcttcatc tattacctct tcttgcaact taaatggagg aattaatact
     6301 tcatcaaagt ctatatttaa atttggaaag ccttctacta tctcttcaca attatcgtac
     6361 tcttcatttg ctaaagaaat gtacatatct aggctctttt tataataaag acctgcaagc
     6421 atatactcac acttcttact tccatattta ataccttctt tataatattt ctcagcttct
     6481 gcgtcatcat ttaattttaa ataaatttga cctaaattat atattgattc aacacaacct
     6541 tgtgctctag ccttttcata ccaaaattta gaatttacat aatctttata gaacatatca
     6601 taaattaatc ctaccatatg ttgagcatac acattatttt cattggcagg tgtatcaaac
     6661 atcattttag actcttccaa tttaccttct ctaaaataaa ttctacctag atgtatacga
     6721 gcattcatat ggtttttgct acttgccatt tcaaaatact ttctagcatt ttttgtattt
     6781 cctaagctct catatataca ccccaatcta taactagacc tcgtatctcc attttcagat
     6841 gctttcttat accaacttat tgcttcttca atattattta aatcttcata gatatttcct
     6901 aatttgattt gacaagatac attatttgga atcctcgaat aatacgaaat cgctttttct
     6961 atatcttgat tctgataata taaatcccct agattttcaa gggaactttt gcatccaaca
     7021 gctatagcat cctcataata tttaatagca ttttcataat ctttttcttc aaaatatata
     7081 cctgccagat tattttttgc ttctgttata tcatcttcag cagccatttt ataataagtt
     7141 tttgccttct ctatctcatt ttctctatta tataaattag caagtctata cttagaaagt
     7201 gtatgatttt tttctattgc tttctcataa taataaattg catctcccag ctcttctttt
     7261 ctatcatata catatgcaag cctatactca gacttcacat catctctatc agcactcttt
     7321 ttataatact tctctgcatc ttctaaatta tcatttattt ccataatcat acccaagtta
     7381 aagcaacctt tcatatgacc tgacttgtac aactgtttat ataaacttac tgttgattta
     7441 ttatctttct tttttccatt taatattgct aaattatatt ttgcatcatc atctaaaagc
     7501 acagaacctt ttttatagta ttcatatgct tcatcatctt tctccaaaaa agtatataat
     7561 acccctaaat taaaacaagc tttacctaaa ttcttgtctg ctgctgtttt taaccatttt
     7621 tcacacctta caaaatcttt cattttaaaa tggcatacac ctaaattatt ttgtgcttgt
     7681 aaataaccta aatttgctat cttttcatac caatatatag catattcata gccatctaac
     7741 ttatagtaca tattacctaa atcaaaagca gcatcttcac ttccactttt atatgccatc
     7801 gaataccact tttctgcttc tttaaattgt tttttacttt catatatatt tcctaaagta
     7861 tattgagatt ttacgtaatt atccaatgca gcactcttat acaactctat tgcgctttcc
     7921 atcttaccca tcttttcaag gagggcaccc ttagtatgta cgactttcgg gtcattagat
     7981 atatttttta ttttttttat agaaaatttt gtatttttca ttattcgcta cctacccatt
     8041 ttataaaata tattctctca aacattatta tacattataa tctacaatta atgctataat
     8101 attgaatttt taccttttgt cactatattg taacattaaa ttttaaattt tataatcatt
     8161 agattttatt atgtaattgg tatataatgt atatataggg gggacatttt atgaatttca
     8221 gcaatatttt tatccaagtt gcagttttat ttataattat tttagttgga tattttgtca
     8281 gaaaattcaa cttattagat gaccactgca catctaagct ttcaactttg acaatgacag
     8341 tttttttacc atcaatgata attagctcca tgcaaattaa ctttgatagt aaaatgatac
     8401 aaaaaatact attgttatta tttacatctt tgattatgta tatagtttca attataattg
     8461 catttttatt gaaatacatt ttaaaatgta atgataaaaa agatttggga atataccagt
     8521 atatagttgt tttttcaaat gttgctttta tgggttatcc tgtaattgaa gcagttttag
     8581 gtcatgaagc aatattctat actgcaatat tcaatttgcc ttttaactta ttttctttca
     8641 ctttaggaat ttatctttta tctaaaggca gtacaagtag aggtttttca ataaaatcac
     8701 ttataagccc tgctacaata gctattgtta ttggactttt tttatttgta acaggactta
     8761 ggttacccca atttataaac gagccacttg agatgttggg aagtataact acaccaatat
     8821 ccatgattat cattggaagc ttacttgcta attcttcagc tctcgattgt tttgtcaaca
     8881 aaaaattata tatagtcact tttataagat tattggtact tcctgtgatt gtgtatttta
     8941 ttctaaaagg atggataaat gataagatga ttttagctat accagtagta atttcttcaa
     9001 tgcctgctgc tgcaaatact gctataatgg ctaatcaata tgactccaat ataacactag
     9061 cttcacaatg tgtgtttttt acaacactat tttcagttat atctatacct tttataagta
     9121 ttttactctt atcttgattt atttttttat gtattatata ataacatatt caataaatta
     9181 caaatataaa cattttattg tagtatattg atggaatttt ttcctagata tttgtcaata
     9241 atatctatat gtattataga ataggaggct taaatatgga tatggattta ctctttaaaa
     9301 tagtatttgc tatagttgct ataggtgttt ctgtaaaaat cattaaaact gttgctagtt
     9361 tagcttttaa aatagcgttg atattgctaa taattttatt tgtatataga atgtttttgt
     9421 aaacatttaa aaataagttt tttaacacaa tagagaagta aaatagatta tctttaatgt
     9481 aaatttttta ttaaagacac tctattttac ttctctatta gcttctttaa attaaatttt
     9541 tattatatct tcccataata taaacatctt caaattttcc atctctgatt acagcatatt
     9601 ttttagttcc ctctatttca aaacctagct ttttatataa atttaatccc ttttcattat
     9661 ccacaattac ttctaatcct actctagtta acataatcca attatctgct atatctaaaa
     9721 gttgttccat aagcttctta cctactcctc ttccctgata attctctgca acacttattc
     9781 ctatttctgc gctatgtctc aatcgtggag aagaatttac atgtaaccca gcaaaaccta
     9841 ttacatcttt ttctccatta ttattagttt cagctacaaa catataatca ttttctccca
     9901 aagaagctaa catagcttca gttttatcga ctctgtcact cattaatgcc aaagtatttc
     9961 tcataacact gtatgttctt ctaatttcat taaaagcttg tgcatcttct cttcttatag
    10021 gtcttattgt tatatctata ggcttactca tattggattc cccctcaaac ataatcttta
    10081 tttttaatta aaattttaca tctttatttt atactgttca aatctatgta tgcttctacc
    10141 tgaggtacat atatcatatt atcttttata tgtgtacttc caaattcttt taattctgta
    10201 ccttctttat ccttagttat tttatatcta atggattcca taccttcaac tacttcagca
    10261 tgaaaaaaca taccttcata ttgtttaaat ctaaaattaa aattgtcttt agtaagtttt
    10321 attatcatat ttttatctac tccttatata attgatattt ttcttattat tagttgtata
    10381 tatactgctt attcatatta atatattata acttatcatt tatttagtat aaagttataa
    10441 atagatatat ttatacttat tttacaatct ttcttcaatt gatatataat atgtttatat
    10501 aaactaatgt atgtgaggtg aaatatgagc aaaaaaacaa aatatttttc tttaattctc
    10561 tttcctattg ctttgctttt aaattttata gccagtaaag ttccaaatat tgttgaaaaa
    10621 tattactccc aattcataga taaaataatt gtacaaatat tgagtaaagt ttctggaatt
    10681 ttcccatttt ctctctatga gattacaatg tacataatag ttatatctac cttcttattt
    10741 ttatgttgta ctttatctac aatttttaat aagaaaaaaa aattaaacat atatttaaaa
    10801 aattccattt taaatatatt atccattgta tcaatagtct actttctttt tgtagtgctt
    10861 tggggaataa actataatag gattccacta gaaaccacac ttataaataa ctataattta
    10921 aaaaacaata cctctatcca atcaaaacaa tatagtgtta aagagcttac taaattatat
    10981 aaattccttg taattaaagc aaatgaaact agaaaactca cattacaaga taaaaatggt
    11041 attgtaaaat ctaatacaga ttataatggt gtcataaata gagctcaact aggatacgat
    11101 aatatcttgg atatattacc tggtgttagt gatagttatt ctaagcctaa gtatattatt
    11161 tcatcaaatc taatgtgcta tacaggaata actggtatat actttccttt tacaggtgag
    11221 gcaaatgtaa atatagcaat acctgactta tatattcctt gtacagttga acatgaaatg
    11281 gcacatcaaa ggggatttgc tagtgaagat gaagctaatt ttatagcgta tcttacttct
    11341 ataaaacacc ccaatattga ttttaactat tctggatata ttttagcttt aaattatact
    11401 gcttctgctt taagtaaggt tgattataat gcatatgttg atatttctgc tggcatttca
    11461 gattctgtcc gtagagattt aaaaaatgaa agtgaatttt ggcaaaaata tgaaggtaaa
    11521 attaatgaaa tttctaatga gtttaacaat tcttacttaa aagctaatgg cgttactgaa
    11581 ggtactcaga gttatggtaa aatggttgat ttattgctaa cttattatga attatatcca
    11641 tataactaaa cactacaatt caaaatttta ttcatataca gctgatatta aatcagctgt
    11701 tttctattta aataaattat ttaacttcaa gcaatcttat ataatttgat agtacttata
    11761 ctatctgtat aacatttcaa ttctaactat tttagttaaa acatttctat tatctttttt
    11821 aataaatgtt ttaactaaaa tttttaaaac ataaaacttc cacattattt cactatctat
    11881 ataaaaatct aaaaattcac atataaaatt acataactaa aaaatcataa atttatagaa
    11941 aacatatatt caaaataaaa aagcatgtat caaaacttga cacatgctta taatcttatg
    12001 tatatatatc attttgtcac aataaaaatt tacttgtaac actgatattt ttaaacaaaa
    12061 tagtccttat ttaattaaat taacattaat taaattcgta ctcattttgc tatcattaaa
    12121 cattacttat agtaattttc taatacacta tttgaaactt ccttcccttg aaaacttttc
    12181 aaccaattct catatgctga gtttttccat acactaccat cattaaatgt tacttgtttt
    12241 acacaagata taatgtactt tacaccatgc tctccagttg attgtttcca tttatcaaac
    12301 aatagaaagg catcattttg attcttacct ggtgctattg cattttgctt tgcatattct
    12361 ttctccattt gtttccattc tttcaaccaa ttttctaccc atgccttgcc ttgttgtttt
    12421 tcaaacatac ttattatatc ctgtggtggt gattgttgca tttttctata agtatgtgaa
    12481 tatgattttg gagacacagg agatatgcct gtaacaatac catagtctac accagctggg
    12541 ctaaatccaa cacttcctac ttcgccatct gctgccacat tttgagcatc ccaatatagc
    12601 tcaagtggtt ttccattttt atcatatgct aagcatgtaa tttcataatc cataattgcc
    12661 ttttcactgc tatttgttaa gcttataaca ttgctaggcc aatgatatgg agtatcatca
    12721 tactgtattt tgctattatt tataaataat ggaatattct cattataaac aagaccttta
    12781 ctactttgaa tatctgatac cttctttgtg acagtactta ccgtatttgc taagtataca
    12841 tttgtttttt ctgaattggt cgtactaaga gaaaatgttg ttgtcgttcc agtaatcagc
    12901 aaagcaacaa ctaaaatagg taagctagat atttttttat atctcacaat tcttcacctc
    12961 tttttctatt aacttataat aaaacaattt accaaaacat taaaatctat gttaacataa
    13021 tttaccaact gttctagtaa ctatatccta tcatagttac tatcaagttt caatactatt
    13081 gatggaaatt ttcactatat taaatctttt tttaatttta taaatttac
//
LOCUS       DAYASV010000104        12935 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYASV010000104 DAYASV010000000
VERSION     DAYASV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12935
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.105"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            178..888
                     /gene="rgaR"
                     /locus_tag="ACGI68_002828"
     CDS             178..888
                     /gene="rgaR"
                     /locus_tag="ACGI68_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HFH0139255.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            1952..2965
                     /locus_tag="ACGI68_002829"
     CDS             1952..2965
                     /locus_tag="ACGI68_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HFH0139256.1"
                     /translation="MNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYIFISDKF
                     DDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNIRLINLS
                     LSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFTLDGDDS
                     FWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEKPGITIS
                     DLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCLDIEENN
                     EKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLTELVERY
                     KNL"
     gene            3098..4396
                     /locus_tag="ACGI68_002830"
     CDS             3098..4396
                     /locus_tag="ACGI68_002830"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFH0139257.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLSHFNKVITTTPNNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(4510..4755)
                     /locus_tag="ACGI68_002831"
     CDS             complement(4510..4755)
                     /locus_tag="ACGI68_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HFH0139258.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            4903..5769
                     /locus_tag="ACGI68_002832"
     CDS             4903..5769
                     /locus_tag="ACGI68_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HFH0139259.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(5773..6309)
                     /locus_tag="ACGI68_002833"
     CDS             complement(5773..6309)
                     /locus_tag="ACGI68_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HFH0139260.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(6500..6703)
                     /locus_tag="ACGI68_002834"
     CDS             complement(6500..6703)
                     /locus_tag="ACGI68_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HFH0139261.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            6994..7758
                     /locus_tag="ACGI68_002835"
     CDS             6994..7758
                     /locus_tag="ACGI68_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0139262.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            8147..9460
                     /gene="prdC"
                     /locus_tag="ACGI68_002836"
     CDS             8147..9460
                     /gene="prdC"
                     /locus_tag="ACGI68_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HFH0139263.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      9865..9950
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            10249..10377
                     /locus_tag="ACGI68_002837"
     CDS             10249..10377
                     /locus_tag="ACGI68_002837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139264.1"
                     /translation="MDNQQVSFEKKKLQRLERNLVPTSVGKWGVPNLWLGNDIVCA"
     gene            10532..12706
                     /locus_tag="ACGI68_002838"
     CDS             10532..12706
                     /locus_tag="ACGI68_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HFH0139265.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTPDVEKPSDGQVPDGEKPSDSQVPDGEKPSDGQVPDGEKPSDGQIPDGEKPS
                     DGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQTPDVEKPSDGQ
                     MPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINKIVYNFWEDEED
                     VKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYHDLRFDDITKTL
                     TFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSPYLPAEDFNGQE
                     NQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWIILGQMDEQERL
                     KQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAGREELKPNDTGK
                     PSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLRLPDGVTAYDGDGNIIGTGEVE
                     ISTQQKFKLKSVVKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKDHSSSLPSSKQN
                     LLSYTIEKKNGQEVNFNIGLPTDDVVNPPVPPTDDTNGHKPKPSPPIDDTVINPPVPP
                     MDDTIINPPVPPTEDAVLNPPVPPTEDAVLNPPVPPTEDTVLNPPVPSVSDTVEKTPE
                     LSRDDTIIKSPKTGDETQLMSYVFISVIAICGLAYQCKIKRN"
BASE COUNT         5216 a         1385 c         2203 g         4131 t
ORIGIN      
        1 tctagaaaag tataggaatt tatcgtaatt taagtttaaa tattaaagag aatttctata
       61 agtagtttaa tcgacaattt aaatttataa agaaatttaa atttgaaagc tagtgagtac
      121 agtttggaaa ttgaaataaa gtaaactaaa tgatatttta gatttggagg tgatattatg
      181 ttcagggtag ttatatgtga tgacgaaaaa atacaaagaa gcatactaaa agagtttaca
      241 gaaaaacttt tgtctgaaag atgtctaaat tatcaaatac tggaattttc ctgtggagaa
      301 gatttaatat ccaaataccc agaaaaaatt gatattatat ttttagatat ccaaatgaaa
      361 gatatcaatg gaattgaggt ggctaaaaaa ataagaaagt ttgatgaaaa agtagaaatt
      421 atatttacaa cggctttttc agagtatgcc ccccaaggtt atgaggttcg tgcttataga
      481 tatttagtaa agcctataga gtatagtaac tttgcaatgg gtgtaaatct ttgtattgat
      541 aaccttcaaa gaaagaagga gcgttatata gttttaaata gtaaaaaagg atttaataga
      601 atactgatag attctatttt atatgttgaa actgtaaaga gagatttagt cgtacataca
      661 ataggtagag attataaagc taatatgagc atgaaacaag cagaaaaatt actgtgtgaa
      721 aatggctttt ttagatgtca tacaagtttt attgttaatt taagaagaat agaagatctt
      781 agggataata tgattactat taatgaacaa tttataccta taagtaagta tagacttaaa
      841 gatttaaaag tagcattaac aagcttgtta tgtgatataa tgtattaatt ttaaaaaggt
      901 aattgaaaat atcgtaaaaa agatttaatg tcatcacagg gctattgatt gaaactgtct
      961 taatatataa aatgttattt atattaagac agttttatta tgcaaaatag cagtttatag
     1021 atttttaata tggtttaaca tgtatatgta gaggttccat tatagtagca tgattattaa
     1081 ttagtagctt gattatcaat atactattat tgatttttat atgcattata tgtattttta
     1141 gaatatgatt acctttttaa aataatgatt atttataaaa ctctatagat atttgtttca
     1201 tttagctacg catactttaa ttaattatat taatatttaa tggagtacaa tataaatatt
     1261 aaagcgacat cttatagttt attgaaacta tagtaatgaa ttaataaata gtttaattct
     1321 atgaaaagat ttccttttat gcaatttatt tattgaaata tgatataata tataaaggtg
     1381 taagtattag ttgttaaaat aatatttata ttagttttta tacatacttg gtataagttt
     1441 ttcatatatt gattaaaatt gatgttgatt tattattttt agtatttttt caccttttga
     1501 ttcatacttt gtagaatggt gtattttcta aaactgatta taagacaagt tcattcaaat
     1561 tagcattttt atatcatata aataattgtt ccccctaaat aagatttaga tgtatagtat
     1621 taaaatcaaa aagtataaaa taaaattgat gcttaatcat agataaatat taataaaata
     1681 tacaagggga ttgtctaata aaaagataat ctcctttttt acaaataatt ctaaattgtg
     1741 atataaatct aaaaatttta tcttaacatt taaccattta atgataataa ttgatataat
     1801 aaatataaat taaatatatg gtgtacttat tttatgtttt tagcaagaaa tatagcattt
     1861 aaaagataaa ttgtgcaact taaatcaaag tttaagatga actattaaca tagatatatt
     1921 aaaaactttt gtaatggaga atatatgtga tatgaataat aaagttaagg ttgcagtatt
     1981 agatacagga atagacaaag aacatgatta cttaaaagat aatttagttg gaggaattgc
     2041 ttttgaatgt atgcatgatt atatctttat atcagataaa tttgatgatg aagatggtca
     2101 tggaactgct tgtgcatcaa taataaagaa agagtatgaa gatgtagagt tatttgtaat
     2161 aaaaattttg agaaaagatg gtattacaaa tataaaagtt cttgaagaag cattaagata
     2221 ccttttagac actaacataa gattaattaa tttaagttta tcagtcatag gagtagaaag
     2281 tgttaagggc ttatttgaag tttgctatga attgtttaga aaaggaaaaa taatagtatg
     2341 ttcactcgct aatgattttg acttaagtta tccagctatg tttaacaatg ttataggtgt
     2401 aagaggattt actttagatg gagatgattc attttggtac aacaaaaaat atgatgttca
     2461 atgtgttatg gatagcaatt catatataag ttgtgatata aataattcct atagattacc
     2521 accaaaatgt aatagttatt tatcagcaaa gcttactggt aaaatagcta agatattatc
     2581 agaaaaacca ggcataacaa tatctgattt aaatgataag cttgaatcat tagcaactag
     2641 aaatcattgg gatagttgtg attttgatga gtgtagtaga attgtaaact ttaaattgga
     2701 tttgtatgac aaagaaaatg ctctttttat gaaagttgct gacgttgtta gggattgctt
     2761 agatattgaa gagaataatg aaaaactctt taagggtagc ctttttaata aggagatagg
     2821 cttggtttat gataattgtt ttaatctgtt gaaaaagcta gagcatagat ttgacataaa
     2881 gtttaattat atggacatct caaaatatga cttagtttca atatatacac tgactgaatt
     2941 agtggaaaga tataaaaact tataaataaa gatttgtagc taaatttaat gtagaaggta
     3001 ttaaattata tgtattcttt gtcggtagat taaaagtaaa aaatatacta taattagata
     3061 gcataagaga aaatgccaat acagggggag ttagataatg aaatatgaag aagcattaga
     3121 gtacatatca caaactaata aattcggtat aagattagga ttagaaaata tcggaaagtt
     3181 attagaatta ttaggaaatc cacaagaaac tttgaatata atacatgtag ctggaactaa
     3241 tggaaaaggt tctgtgtgtt cttttgtttc taatatacta agagagtgtg gatataaagt
     3301 tggtttatat acatctccat atttagaaac ttttactgag cgtataagag taaatggaca
     3361 aaacatacca caggatgatg ttgctagaat aattgagtta ataaaagaaa aaatagagat
     3421 tatggtaaaa gaaggctatg catatccgac agaatttgaa gtggttactg caatggcatt
     3481 ttattactat tcagaacaaa aggtagactt tgtggcatta gaagttggtt taggtggcag
     3541 atatgatgct acaaatataa taacaaaatc acttgtaagt gtgatagtat ctataagttt
     3601 ggaccataca ggtatacttg gagatacaat agaaaaaata gcgtacgaaa aggctggaat
     3661 aataaaggaa aatggagtag tactagtata tgaccagact gatgaagcaa aggatgttat
     3721 aaagtcagtt tgtaaagaga aaaaagctaa atatatagaa gttgattttg atgatataaa
     3781 cataaaaaaa tcagatataa attctcaaat atacgattgt actgtaatga aagaaactta
     3841 tagagacctt gaaataaaat tgataggcga acatcaaata aacaattcta tcttggcaat
     3901 tagtgtaata aaatatctaa aagacataaa taaattagca aatataaatg aagaatctat
     3961 aagaaaagga cttataaata ctaaatggcc aggaagaata gaaaagataa aggaaaatcc
     4021 aatttttata atagatggtg cacataatga agatggagca aaatctttgg caaaagcact
     4081 tgataagaat tttaaaggta gaaaattaac cttattaata ggtatgttag aagataaaga
     4141 tattgatagt gttttagaga tattgttgtc acattttaat aaggtaataa caacaacacc
     4201 aaataaccca agggccataa attcagatat attaagagaa aaagtattaa aatatgtaga
     4261 tgatgtaaca tcaaagcatg agatagagga tgcagtaaat tatacattag aaacatcaag
     4321 tgaggatgat attataataa gtgcaggttc attatatatg attggtacag tgagaacttt
     4381 agtaaagaaa ttataatttt ataaaataag tatacaaata aaaatataaa aaagagttgt
     4441 ctaaaaaatg gacaactctt tttatataaa ataagttttt aataatttta ctgatgttta
     4501 tataagtaat tatatagatt gacgtatagc tttagataat tggtctgggc aagaagtacc
     4561 ttttccacca cagtcaatac catttaattt atcagcaact tcaagagcat tttggccaat
     4621 tattaagctt ctaagtcctg ataaattacc attacatcct cctataaact cagcatcaac
     4681 aatcacatta ttttcatcaa cttcaaaaat catttctcta caacatacac cacttggatt
     4741 aaatgttact ttcattaaaa agacctccat taaatattat ttcttttttt aacattaata
     4801 ataataccaa atatatgcgt aataatgcaa ctataaagca taatacaagc taatgaaata
     4861 atatattatt gtagtatatg tattgtagag aggaggggtt atatgaaaat ctatatttct
     4921 aactacttat caaagagtat tagcatagtt gattattcta cacttgaact agaaaaagaa
     4981 atagtattag aagataatat atatccacac catttttgca tagaaaaaga aaaaaattta
     5041 atatacatac ctagttcaag taatgggata ctctatgtct tagatttaag taatgataaa
     5101 ataattgata ctgtctctat aggaggaagt cttagtcaag ttgtacttag cgataatgaa
     5161 ttgtttgtgg caaatgaaga ttctaatagt atatatgtac tagataaaaa cacattaaat
     5221 ccaataggaa ttattggagt tgataatatg ccacatggat ttgactttga tagagaaagc
     5281 aaaaagttat atgtaccgtg tataaactcg atagtatgta tagatacaat taataaaaat
     5341 atacaaaaga aaataaatat ggactttaga gcttggcata tagaacttga taaacaaaag
     5401 aaagaaatgt atatatcaac acttgatgga aagcttataa taatagatga agtaagcatg
     5461 gatataaaaa aggtattata tgaattttta ttaccagtag aaataagatt taattatagt
     5521 ggcaaaaaag tttatgttgc tgatttgggt tataataatg taagaatttt agattataca
     5581 acgagtagat acattggaaa tattgaaata aatgggattc cccaaggatt agaaatatct
     5641 aaagatgaaa agttactatt tgtatcagat acacaagaaa attcagtaaa agtttatgaa
     5701 actgcaaata acaaattaat aaaagaaatc aaggtaggaa aagagcctac aactataatt
     5761 tgtttgtagg ctttaatgta tgtctcttat aagtaaatcg ataacttcgg catacatata
     5821 agatgcatta tttttccagt tattgcaaat aagttttgct tgtttctctg atgaaacatt
     5881 tagatttaaa tctattaagt tagactgatt ttctatgact ctaaggttga caataaattc
     5941 attatcactt tgttttacaa aactagactt tacttgagta tctgctagta gattttcttt
     6001 gttagaatct atatacttgt ctattttttc agtaacacta gtaggtattc gattaaagaa
     6061 gtattctaga ctttctacgc ctctttgagt aagagcataa tattctctgt cagagtcttt
     6121 atatgtagtc aaaaacttag attccataag ctgagataaa agctgttgta atgaaaaata
     6181 attcatcatc tcagtctcta atactacttg agttatttga gaattagtta aatccatttt
     6241 aattttttct aatatatata aaattaaaag tttgtgatag gctaattctt cagacgagtt
     6301 ttcaaacata aatatcatcc tccgtcaaac tatttctata tataatatat taaactaatt
     6361 tgtacaaaat taaaagaaca atttgtattg tattattaat ctttaataat tcatataaaa
     6421 acaattttat aaataaatat agaaaaacag ggctgctaat tagcagccct gttgataccc
     6481 aggtgtcaaa atttactatt tattttccag ccatttgtct ttcagccatt tcaactaatt
     6541 tcttagtcat atatccacca acatatccat tttctctagc tgttaagtta cctttgtcta
     6601 tgttttcata gttagataaa cctatttcgt tagctatttc taatttcatt tggtttaaag
     6661 cagcttttgc ttctggaact actgttctgt ttgaatttga catagtaaat ttcctcctca
     6721 aataattagt ttatgatgtc taacaacact tttatttatg tgatgttaat actaatttct
     6781 ccaaaataaa aataatatat acagaaaaat aaaggtaaaa atgtaaaaaa tataaaaaaa
     6841 ttaaataaat gagtatttta gagagtttaa ttaggtcaat ataggtttta tttttttagt
     6901 tattgtctta taaattaaat attacagtac ttttttgagc tgaaaaaaaa taaattttaa
     6961 atatagaaga aattatttca aagggggaat agtatgaaaa gagaagttaa aaggactaaa
     7021 gctttgatat atagtgtttt atttttttca ttattattag tgttaatatt tgtttttgtt
     7081 tatggaataa aatacatatg ttctaaatct gacaattcaa aaatgcctat atataaagtt
     7141 gatacaaatg agaaaaaaat agctttgagt tttgatgtag catggggaac tgataatatg
     7201 gatgatattt taaaaatttt agataaacat aatgtaaaag ctaccttctt tttagttggt
     7261 agttgggtgg atgataatga agaaattgta aaagctatag atgaaaaagg acatgaaatt
     7321 ggaaatcatt caaatactca tgccaatttg aaggaaatat caaaagaaga cataacaaaa
     7381 gagatagaaa ctacatcaga aaaaatatat aatataacag gaaaaaggac taatttattt
     7441 agacctcctt ttggggacgt aaacaataaa gctatggaca tttgtagtga tttaggttat
     7501 aaagtaatta agtgggatgt agattctata gactggaagg agcttggacc aaatcatgta
     7561 attgaaaaag taataaaaga atctcaacct ggttcaatag ttttattcca tgctaatata
     7621 aatgatgtag ataactattt ggatactata ataagtcgac taaaaaaaga tggatacagt
     7681 ctagtcaaaa tatctgattt gttgtataaa gataattatg tagttgattc caatggagtg
     7741 caaaaaacta agaattaata agtaataaat gtatatttta agtaaagttg tgataaatta
     7801 tatagtatat gttagaaaag tgtcgataaa aagacggtgt ctattttttg acacttttta
     7861 tacatgtatg caatatcaat aattatagat taaattgatt aaaaaagtgt ctaataattg
     7921 actttttttt gatgggttta aaaatttaga agaattaatg aatgctgtaa gacagtattt
     7981 ccaaagggta taagtttgtt taaaagttgg catagaaatt gctttgttaa taagcgatat
     8041 gttctataga aataaacaat atgttaagac cttataatag aaaaatagca ttaacattat
     8101 ttgaacagct tataaaaaaa ttaatatact taagggggcg tactagttga acaaactata
     8161 ttctatttta ttaaaacaac acgttggtgg tccagataag ccagtagtaa gtgttggaga
     8221 tgtagtaaaa aaaggaactc taatcgcaga accaactgga ttaggggcaa atatttatgc
     8281 cagtgttagt ggaaaaataa gtgaaataaa tgaccaagcg atagtaattg aagctgatga
     8341 agtacaagaa gatacttttg aaccattaaa aggagaaggt atacttgact taattaaaga
     8401 agctggagta gtaggaatgg gtggagcagg attccctact catgtaaaat taaacataga
     8461 tttaaatggt ggaacaatat tagcaaatgc agctgaatgt gaacctttat tagctcataa
     8521 tataaaagaa atagaagaaa gaccagaaat agtttatcaa ggtataaaat atgctatgga
     8581 agttactaat gctggaaaag gtatgctagc aataaaaagt aagcatccaa aagcaataga
     8641 agctttcaaa aaagtaataa agccaggaga caacatagaa gttgcagaac tagttgatat
     8701 gtacccaatg ggagaagaga gagcaatagt aagagacgtt ctaggaaaat tacttgagcc
     8761 aactcaatta ccttcagagg ctaatgcagt agtaataaac gtagaaacac taactagaat
     8821 agtagaggct gttgagcaaa aaagaccagt tatatctaaa aatataactg tagttggtca
     8881 attaaacagt ggaaaagaat ctatagtgtt tgaagatgtg ccaattggaa caactgtcgg
     8941 agaattaatt gaaagagctg gtggaataaa aggtgaatat ggtgaaataa tcctaggagg
     9001 acctttcact ggtaaagcta ctactttaga tgctccaata actaaaacta gtggtggtat
     9061 aatagttact atgccatttg ttaatgaaaa gagaaaaatg ggtcttttag tttgtgcatg
     9121 tggacctaat gaagaaagaa tgagagatat agcaactaaa atgggagtta ctgatatagt
     9181 atcagttcaa aaatgtaaac aagctcaaga aattaaaggt gcattaaaat gtgaaaatcc
     9241 aggacactgt ccaggacaag ctcaaaaatg tattgaattt aagaaagctg gagcagaagt
     9301 tatattaata ggaaactgta ctgactgtag taacacagta atgggttctg caccaaaatt
     9361 aaaattaggt gtatatcata ttactgacca tgtaatgaga acagttaatc acccattaat
     9421 aagaagaata aaaggtgacg taagattaaa aataaaataa tagataaata gaagttagac
     9481 atctttagaa gtaaaactta aaagttactt ctaaggatgt tttttaatgt attttgaggg
     9541 caatatatat attgtaaaat aaatggaaaa aatagacaaa atatgctaga tattgtataa
     9601 tgataataat agctattaca attaatattg gacaaaaata atgatactca agtatgtggt
     9661 ttgtattaat atgcatatta tattaagtca actatccgat agagtaaaag gtttttgtga
     9721 tatttgaaga tactctatag tatctttttt attataaaaa ctaatttttg tcttttatta
     9781 gataagtatc taggatacta atttatattt tattgagttg ttaaaaaagt gcacttaaaa
     9841 agttaaaaaa gaaaaattaa aaaacctatt tatagaaact gtgaagtata tcttaaacct
     9901 gggcacttaa aagatatatg gagttagtag tgcaacctgc tataaatata aataggagta
     9961 acttttaatt gtcaaaaata tataaaataa atacataaat aagtaattaa tagaagatag
    10021 atataaatat atttttgaaa tgctccctgc attcataacg agtgtagagt ggcagatatc
    10081 tgcccaaaac tcctttaatt gctggaagtt cctaaagcta actaaaccac aacataatca
    10141 tgaaataagg ataagtgtga tagagtgtgc atttaataag cacttgaggt tacgaaagta
    10201 gaaaaaatta gttagatagc ataaggttaa atcctaagtg ccttgaaaat ggataatcag
    10261 caggtaagct tcgaaaagaa gaaacttcaa cgactagaga gaaatctcgt acctacaagt
    10321 gtggggaaat ggggagtacc taatttatgg ttaggtaatg atatagtctg tgcttaatag
    10381 aaatattaag aagttcatag gagaactggt taggagtaac gaccctaatt gaacacacta
    10441 aagatgtatt attgaataag attcatataa tatttaataa ttttaattat attaagtgaa
    10501 tacaatatat gtttttagta tcaggagaat tttgaaaaca aaaattaaaa aatcaagtat
    10561 aatcagttta gtaattgctt ttagtatggt gtttactgca tttacaccaa tagtatctta
    10621 tgctgacgaa gtgactagca atgatacaat acttaatgga gaagaacaat caaatggtca
    10681 gacaccagat gtagagaaac catcagacgg tcaggtacca gatggggaga aaccatcaga
    10741 cagtcaagta ccagatgggg agaaaccatc agacggtcag gtaccagatg gggagaaacc
    10801 atcagatggt cagataccag atggggagaa accatcagat ggtcagatac cagatggaga
    10861 gaaaccatca gatggtcaga taccagatgg agagaaacca tcagatggtc agataccaga
    10921 tggggagaaa ccatcagatg gtcagatgcc agatggagag aagacatcag atagtcagac
    10981 gccagatgta gagaaaccat cagatggtca gatgccagat ggggagaaac cattagatga
    11041 gcaaactcca gaagaagaaa aaccattgga agaagaaata gttatagagg aaatgagttt
    11101 aaaacaagat attgataaaa ttcttgatat gactttatct caaataaata aaatagtata
    11161 taacttttgg gaagatgaag aagatgtaaa ggcagatgaa caatcagaaa ttaaccaagt
    11221 ttttacttca gaagactcat ttattagctt atggtatgac aaaaaagcaa aagtaaaaaa
    11281 ttcttgttta ctaaaagaaa tagatggttc agatagacct tatcatgatt taagatttga
    11341 tgatattaca aagactttaa cttttgacta tgtaataaag ggtcttgtag gagcaagctc
    11401 aaaagacgac aaatatgtaa tagaaggtga agatggagag caaactgcat tttgttacaa
    11461 taatcattta agaccaccaa gtagtaatgg aaagagtcca tatttaccag cagaagattt
    11521 taatggtcaa gaaaaccaaa atgaagaagc agttaaatct atactatatg caggttctga
    11581 atttgatgga tttggatata agcaacaatt taatttaggt ggagaagaaa atgaattgat
    11641 gacttactca gctactcaaa gtgcaatttg gattattctt ggacaaatgg atgaacaaga
    11701 aagacttaag cagtatcaag gttctataaa cctttgtgat aaacttatag aaagagcaaa
    11761 aactgaagag gaaagagctg agtatcagaa gaagaaagaa gtagctataa atataaaagc
    11821 ttatttagaa gcattgttaa aagcaggtcg tgaagagtta aagccaaatg atacaggaaa
    11881 acctagtttg agtaatggtt taactaagat taactttgaa aaaaatgaag atggtactta
    11941 tgaaacagaa gctgtagcct tagtaggtta tagtggtgta gtaaagttaa gactaccaga
    12001 tggagtaact gcttatgatg gagatggtaa tataataggt acaggtgaag tagaaatttc
    12061 aacacaacag aaatttaaac ttaagagtgt tgtaaaacca gatgccaaag ctaatatttc
    12121 tgctgtatct tatgattata tttttccaaa agctattcaa tattataagg cagtgcttga
    12181 tttaggacaa aaagaccatt catcttcatt accatctagt aagcaaaatt tattaagtta
    12241 tacaatagag aaaaaaaatg gtcaagaagt taactttaat ataggtctac caacagatga
    12301 tgttgtaaat ccaccagtgc caccaacaga tgatacaaat ggacataagc caaaaccatc
    12361 accaccaata gatgatacag taataaatcc accagtacca ccaatggatg atacaataat
    12421 aaatccacca gtaccaccaa cggaggatgc agtgttaaat ccaccagtgc caccaacgga
    12481 ggatgcagtg ctaaatccac cagtgccacc aacagaggat acagtgttaa atccaccagt
    12541 accatcagta agtgatacag tggaaaaaac tccagaacta tcaagagatg atacaataat
    12601 aaaatcacca aaaacaggtg atgaaactca gctaatgtca tatgtattta ttagtgtcat
    12661 tgcaatttgt ggattagcgt atcaatgtaa aataaaaaga aattaatcta gattattatt
    12721 aagtttaaaa taaaaagtct caaattgatt tataagtcat tttggggctt ttttatgtaa
    12781 atataatatg caaatatagt ttgtttaaaa atatatattt taattatatt cttaaattta
    12841 tgattggata caggcattca ttaattgttt gatgaataga aaaatgtttt gaaggtgaaa
    12901 atagtattga aaaaataata tactaagggt ataat
//
LOCUS       DAYASV010000105        12924 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYASV010000105 DAYASV010000000
VERSION     DAYASV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12924
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.106"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            108..296
                     /gene="rpmB"
                     /locus_tag="ACGI68_002839"
     CDS             108..296
                     /gene="rpmB"
                     /locus_tag="ACGI68_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFH0139266.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(456..608)
                     /locus_tag="ACGI68_002840"
     CDS             complement(456..608)
                     /locus_tag="ACGI68_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HFH0139267.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(706..1182)
                     /locus_tag="ACGI68_002841"
     CDS             complement(706..1182)
                     /locus_tag="ACGI68_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0139268.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1401..2162
                     /locus_tag="ACGI68_002842"
     CDS             1401..2162
                     /locus_tag="ACGI68_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0139269.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLCYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2422..4350)
                     /locus_tag="ACGI68_002843"
     CDS             complement(2422..4350)
                     /locus_tag="ACGI68_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0139270.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILNEPIYWSENSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4563..5036
                     /locus_tag="ACGI68_002844"
     CDS             4563..5036
                     /locus_tag="ACGI68_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0139271.1"
                     /translation="MNGVEKYMVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNK
                     EALLKDLLKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRY
                     VFSLLAPHKNEGNIHLKTLSKLATCLMEDDFTSEVKNASDASHLVKYITNKMKEE"
     gene            5039..5347
                     /locus_tag="ACGI68_002845"
     CDS             5039..5347
                     /locus_tag="ACGI68_002845"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0139272.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5461..6567
                     /locus_tag="ACGI68_002846"
     CDS             5461..6567
                     /locus_tag="ACGI68_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0139273.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAV
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6642..9320
                     /gene="mngB"
                     /locus_tag="ACGI68_002847"
     CDS             6642..9320
                     /gene="mngB"
                     /locus_tag="ACGI68_002847"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFH0139274.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDKYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIVKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPASLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9637..11973)
                     /locus_tag="ACGI68_002848"
     CDS             complement(9637..11973)
                     /locus_tag="ACGI68_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0139275.1"
                     /translation="MLDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGG
                     AENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDT
                     YDGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVL
                     DTTKNDASKENSDENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSY
                     GVNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYG
                     YGNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTL
                     SRIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNL
                     TYWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKTVINQIRTINL
                     KEKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQL
                     LDVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNV
                     SHDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSG
                     DVYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENL
                     IINISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTE
                     GSGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11945..12661)
                     /locus_tag="ACGI68_002849"
     CDS             complement(11945..12661)
                     /locus_tag="ACGI68_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139276.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         4820 a         1796 c         1674 g         4634 t
ORIGIN      
        1 tatgtgtaag gcaaatatat atttctattg caaatttttg ttatttatga taatataaat
       61 atgtttttag tctttaaata gttatcattt taaggaggtg tgcgtagatg gcaaaagtat
      121 gtagcgtatg tggtaaaggt aaggtttctg gaaaccaagt atcacactca aataaacata
      181 ataaaagaac atggtcagct aacttaagaa gcgtaagagc gattatagat ggagctccta
      241 agagagtaaa agtttgtact agatgtttac gttctggtaa aattgaaaga gcttagtaac
      301 cacgtttaaa agcctacttc cgtaagtagg ctttttaatt taatcatttt tactctttct
      361 cataaatttt aggattatac ttgaaatcca ttttggtaat tttattgtta ctactttcat
      421 caatactcac cccacacttt atagttaaaa ctattttaac aaaaaaataa taatgctcca
      481 catccaataa gtattaatgc ggtaagtgct gaccaaatcc agccaggaag caacattgac
      541 agaattacag ccattcctgt tgctagacaa agtattccta gcaatacctt agacttgtta
      601 tttctcatgg aacacctcca catagaacct gtatctataa atattttcat attcaagttt
      661 atgcgacagt tttccatttt agaccaataa acacattatg taattttaat ttttcatact
      721 agaattatta ctgaactccg atatagattc tacaatatcc aaaattcctt tttttctact
      781 taacctttca ggtccactat ataaaacaac tggtataact ttaggaagtt taaaattttt
      841 atcttctatt tcttcaaggc tttttttcat aataaaactt ttccaaattt gttctctata
      901 cgatttcaat cttgggacaa tttcagtatc catttctgtt tgaaattcta acactatata
      961 atgtgatact ttgccgcttt tagaaaatac cttataaatt atatcaactt cttttccttt
     1021 atattcacat ataaaagatg tttctaactc tatattttta agagttaatt cttttgctat
     1081 tgagacactt gtaaaatttt gaatcaaact taaaaaactt tctttatctg aatacattct
     1141 tctgtactct tctcgttttt cattgcttct ttttaaattc atacagttcc tcctagacta
     1201 tagaccttct attttttagt aaagatagtc tataaatttt attttttaat tttcactagt
     1261 gagtattgac agttgtgtgt aaaatatcca gtaaagatat ttttttatat ttttattaaa
     1321 agatataaat ttttatattt taatgtattg acatttacgt tgcgtaaaag tgtatattta
     1381 atttatagga ggtactactt atggaatata cagttcaaaa attaagtaaa ctagctggta
     1441 taagtactag aacacttaga tattatgatg aaataggtct tcttaagcca cttaaaataa
     1501 attcttcagg ttatcgtata tatggtcaaa atgaagttaa taaacttcaa caaatacttt
     1561 tttatagaga attaggaatt aacttagaaa atataaaaaa cattataaat tcaccaaact
     1621 tcgatagttt aagtgcgtta aaagaacacc ataataaatt actagccaaa agaaagcaaa
     1681 ttgatttact aatagaaaat gtaactaaaa ctatagctct gaaggaggga aaatacacta
     1741 tgacagatat agaaaaattt gaaggtttta aagaaaagat gattgatgaa aatgaaaaaa
     1801 attatggaac tgaaattaga gagaaatatg gtaaagatat tatagataca tcaaataaaa
     1861 aacttaaaaa tatgtctaag caagattatg aaaattggca aaacctaaat atcgaaataa
     1921 ttagtaaact taaaaaagct tttaaaactg gtgatgcctc tagtgaactg gctcaggaag
     1981 ttgcaaaatt acatcataaa tggttgtgtt acacttggaa cacatactct aaagaagctc
     2041 attcctctct agcacagatg tatgtatatg atgaacgttt tacatcatat tatgatagag
     2101 aacaacctgg attagctaag tttttaaggg atgctatagt aatatataca agagataaat
     2161 aatataaaat aataaaggtg gtgtctcaaa atgaactttt tagttcatga gacactcttt
     2221 tttgtatgaa atcagatata tttttatcat tttatcaatg aaaaacaggt ttctctctct
     2281 attttgagac agaatcttta ttgtataata attataatca atacaaaata ataacaaatt
     2341 atacaaaact tacttatact atttatgcta aaaacaaaaa atattttatt aaagttttta
     2401 aaacttatgt aatataaaaa tctatactat agacttgatt atggaaagaa aattatcaaa
     2461 atcattacaa ttagatatac tatctctaat atcattgtca tgaattatct ttaaaaggat
     2521 atcataaatg gagtttattt ttatataatc atctttattt atagccaaca tcatgattaa
     2581 tttaacagta gaattttcac tccaatatat tggttcattc aatatggaaa tacaaatctt
     2641 agttcttata gagcttaatt ccataggatg aggtatagct aaaaaatcat ctatattggt
     2701 agaagataaa ctttctctgt aaaatacaga ttcagcaaat gatggaaata caatttcact
     2761 ttgagttaat ttgttgcaca tcaactttat aagctcatct ttatctttga ttttaggatt
     2821 aacaataaat aaatttttat caaagaaatt gtccaaaaga ttactataaa tatgggaatt
     2881 attagtgatt gacttagata tattctctat atcttttttt aatagtttta aatccactaa
     2941 tacaatagga atactatcat gatttaaagg aattgtcgat attattatat ctacatctga
     3001 caattcacta gctaaaaatt gattaaatga tagacattga agtatattta ttttatcatg
     3061 aaaaactttg tttaattgaa cctctaataa cctacttgaa ccataccctg aaccacatac
     3121 taatactacg tttttacact taatattact ttgaaaaaat ctctctattc cagcacctat
     3181 atgcagggat acatatccaa tttcatcttc tgttaaagag taaatactat ttttaaaaac
     3241 tttttctata gcatttaaag ttatttcaaa tgctaaagga taattactct ttattgtatt
     3301 tataagggga ttcaccttat ttaaattata agatttagaa ttgagaattg atttaaagtg
     3361 cagtactaaa tcatcaaata atacactatc atttctaagg tcaaaagtat attgattgtt
     3421 gattacttct aaaatttctt caacatactc tataatttta gtatctaccc tattggaaac
     3481 gttatccaaa agagaacttt tagttataaa atgattatat agataaactc tgtctgcctt
     3541 tgaaataatt atagtatatt tactctcaat ataattaaat atattttcaa cagttgtatt
     3601 cgattgaaaa tctgttaaaa caacatctaa aacattatct aagcagtaac catcaagtat
     3661 tctacttaca gtaatagcta aatggataat aaagtttttt agattaaaat ctaggaaatt
     3721 gatattaaat ttattaattt cacttaacac taccctttga agctctatta aatctacatt
     3781 attaaataat tctatttctc tatctgtaat tccaattata tagttttcaa aattcctatt
     3841 tatcatatta ttagaaatac atcttcgaat atctatttcc ttaccttcaa ctttaactcc
     3901 ataatatggc ttagaaacta atattaaatt atactttgac aaaataggct ttattctttt
     3961 tatgtcattt agtattgtaa atttactcac atacaagcta ttagctaaat cttctagttt
     4021 tatatatgta ttgttataga gaatatattt taaaatatat tgatttcttt ctataggaga
     4081 atctggtatt tcacttgaat ccataatatc atcagatatt aatgctagat actgttggta
     4141 taatgctaaa ttaagaatat ctatataata tccttctcct ttttttattt taataatagc
     4201 accatttttc tctaaagttc catttataga agtaatatca tttcttatag ttctctcact
     4261 cacttttaaa tgattactta aagttttaca agtaatttta tcatcacttt catttaataa
     4321 tttaaacaac gtgagcaatc tcttgtgcat aaatatcccc ccttatagaa tttcttctaa
     4381 attatactat ttacactcat ataatacaat tactctaata aaattgtatt ataataattt
     4441 atttccactt ctatatggaa attagttatt atcataatat aaatttccat gtcgaagtgg
     4501 aaattagtta tatttaattt aattaggata atgattacaa tataaatata agttaagaca
     4561 ttatgaatgg agtggaaaaa tatatggtac tacaagaaaa atatatattt atagataaag
     4621 acttaaaaag caaagaagaa gtattaaatt ttattgcaga tagtgcttat atactaggta
     4681 ttacaactaa taaagaggct cttttaaaag accttctaaa aagagaaaat gaatttccaa
     4741 caggaataaa agatggattt gcgatacctc atacaaagag tgtaaatgta aaggaagctt
     4801 ccatactcta tattaaaaca actaacgagt tagaatggaa aactttagat gatagtaagg
     4861 ttagatatgt atttagtttg ttagccccac ataaaaatga aggaaatata catttgaaaa
     4921 ctctatctaa attagccaca tgtttaatgg aggatgattt tacaagtgaa gttaaaaatg
     4981 ctagtgatgc aagtcattta gtaaaatata taactaataa aatgaaggag gaataattat
     5041 gaaaatagta gctataacat catgtatagc aggattagca cacacaccta tggcagcaaa
     5101 agctttagaa caagctgcat caaaaatggg tcatgaaatc aaagtagaac aacaaggtgc
     5161 tatgggacga gtaaatacta taacaaaaga agaagcacaa gatgcggact ttatacttat
     5221 agcatctgac caaactatat ctggaatgga aagatttgat ggtaaaaaag tgataaaagt
     5281 caaaataggt atagcgttaa aaaaaccaga agctgtctta acaaagtgta tagaagctat
     5341 ttcttaataa aaaaatctca ctctaatata gaatgagaaa aatcaaaaat taaaaaattg
     5401 tctaatgtaa ttataacata ttatcaaaaa atataacaaa cttatatagg aggattaaaa
     5461 atgagtaaaa aaaataaatc tcttttaagt gaacttaagg gacacttaat gacaggtatc
     5521 tcatatatgt tgccacttgt aataggctca tctttagttg tagctatccc aaagcttaca
     5581 gctttagcag tgggtataac aagtttagat gcttataaaa atgtatctgg tttttatcat
     5641 attctttact taatggaaca agttggttgg actggcatag gtctattaaa tacggtatta
     5701 gcaggattta tagcattttc aataggtgag aaaccagcta tgggtgcagg ttttattggt
     5761 gggctcatag caagtaatac taatgcagga ttcttaggag cagtaatagc aggtttcttt
     5821 gctgggtatg tttgtaagtt tttaaagaaa aaaattaaaa tatctgggtc agcatcagga
     5881 atgatgccac ttataataat gccacttata acagttggat taactggttt tttaatgagt
     5941 gttgtattag caggaccttt aggaaacata aatacttcat taaatgcttg ggtagctgaa
     6001 atgtgtcaaa atggaacaaa ttcggtggta ttggctttaa ttttaggcgc aatgatagga
     6061 ttcgatttag gtggaccagt aaataaagca gcttggatgg caggaaatgc attattgtta
     6121 gagggaatct atctaccagc gatattggta aactgcgcaa tagttatacc tccactagga
     6181 tatggtttag ctacattcat aagaaaatca aaattttcca atgcattagc tgaaacagga
     6241 aaaggcaata tagttatggg tataattgga ataacagaag gtgcaattcc tttcacttta
     6301 acaaatccat taaaattagt tcctgttaat atgataggat gcgctttggg tgtaggatta
     6361 tcagctttgt taggtgtaca tgctataatg cctccagttg gtggattata tggatttata
     6421 tcaataggtt ctggatgggc ttacttagta ggtgcaatat ttggagcttt agtaatagca
     6481 atattatcaa caactttagt agacttcaat gaagatgaaa ctacggaaaa tacagaattt
     6541 gatgaaatag agttagatat aatgtaaatt aaaaacttaa ttttagataa ttgagaattg
     6601 agtgtactct caattctcaa ttaaattttg ggaggatttt tatgaaaaag acaaaagtac
     6661 atgtagttcc acacagtcat tgggatagag aatggtattt tacaacatca aggtcaaaaa
     6721 tatatttaat gaaagatttt caagatatat tagacatatt ggaagaaaaa gaagacttta
     6781 aatactttac tatggatgca caagcttctc tcttagatga ttatttaaaa tggagacctc
     6841 aggataaata tagaataaga gatttagttc aaagaagaag attagttatt ggaccttggt
     6901 atactcaaac agaccaattg gttatatcag gagaatctat aattagaaac ctttactatg
     6961 gaatagatag atgcaatgaa tttggtgaac ctatgagggt tggatatgta ccagattctt
     7021 ttgggcaatc agctcaaatg ccacaaatat acagaggttt tggaatagat agttcattat
     7081 tttggcgtgg agtttctgat gatatggtag atacaacaga gtttatatgg aaaggttctg
     7141 atggttcaaa agtattagca gttcaaatac catttggtta ttactatgga ggtaatatac
     7201 cagaagatga ttcagattta aaagaatatt taaaagatat aatcgtaaaa cttaaggtta
     7261 aagcttctac taataacgta tattttccaa atggatttga ccaagcacca ataagaaaaa
     7321 accttcctga tatattaaaa aaagctaatg aaatagataa tgaaaatgaa tacgaaataa
     7381 gtgatataga aaactatata gattcagtaa gaaatgaaag aaatgaattt acagagctta
     7441 aaggtgaatt tttaaatgct aaacatatga gaatacacaa atccatattc tcctcaaggt
     7501 cagaccttaa aataatgaat aacaaattag aaaattatat tacaaatgta cttgaaccgt
     7561 tattaacatt aagttattca cttggaaatg aataccctca cctagttata aaagatatat
     7621 ggaaacttat gtttgaaaat gcagctcatg acagcatagg ttcttgtaat agtgacacaa
     7681 caaatgagga tgtatacaca agatacaaac aagccagaga tttgagtata aatttattag
     7741 atttgcatat gagacttata agtactcaaa tcaaaaataa taatgaagaa ataacattaa
     7801 cagtattcaa tggattccca gaagtcagaa atgaagttgt agaatttgat acttatattc
     7861 caggtcaaga ttttgtgata aagaataaaa atggaaaaat tcttaattat ataataaaag
     7921 aaaaagaaga tattacaaat tatatattaa ctcaaacaat aagattaaac ccaagtaaaa
     7981 aaatatatat acccaaaact gtatatagag caaaaattgc actttcagta aaagatatac
     8041 caccaatggg atatactcaa ctagtttttg aattggatac taaaaataaa tgtgaaatag
     8101 aaacaagtaa ttgcaaggaa atagaaaaca aatactacaa aataacagcc aataaaaatg
     8161 gttcattaga aatattagat aaagaatcta ataagactta taaaaaccaa gctgtaattg
     8221 aagacaatgg agatgatgga gattcataca actattctcc tccaagaaaa gatatttatg
     8281 tttcatcttt agattctgta tcttcagtag aagtgctaaa atcaaacatt caagaagaaa
     8341 tgatacttaa atattcattg accataccag cttcactgga agaaagggct ataggtaaag
     8401 tttctgtgaa aatgccagta acaatgaaaa tcaccttaga acctaaagaa caaataatta
     8461 agtttaatgt taatataaaa aataaccaag cactatctca tagagtaaga gttttattta
     8521 atacagaaat agcatctaag ttttctatag cagatcaaca atttggaata atacaaagac
     8581 caacagtatt agaaaaagac cttgcattgt ggaaaagaga caattactca tggcaagaaa
     8641 aacctataac tatcgaacca atgcaaagtt ttgttacttt agaggatgga caaagaggaa
     8701 tagcattaat aactggttgt gtaagagaat atcaaatagt tggtgataat ttagatacaa
     8761 tagcattaac actattcagg tcatttggat atatgggaag agaaaattta ctttatagac
     8821 caggaagagc ttctggagaa aaaataatag caacaccaga tgcacaatta cttaaagagt
     8881 taaactttga ctttggtctt tacatatata acaataaatt tgatgaagca aatgtagcta
     8941 atacagctaa gagattctta acacctatac aaatatatga atatgctgat tttttaaatg
     9001 gtagacttat atttgcattc aatgacgaaa aacaaatata tgaaaatgaa tttagtttga
     9061 tgaatgtaaa taattcaaat ttcacagtaa gtgcaataaa gaaagaagaa aaaggagatg
     9121 gaattgtagt tagaatcttc aatggtatga aaaatgagga tgctaaggga agtcttaaca
     9181 tcaataaaaa tgtcaatgat gcttatagtg caatgctcga cgaaatctat gataatacta
     9241 gcaagcttaa agtaaatact aaaactgtag aggtaaattc tttatcacat tgtaaagtac
     9301 aaacaatagt aattaaataa tataaaatag cttatacaag atttcaaaat aaattgctag
     9361 taccatgtta atagtaaagc cagatattat gctatttata agctctgtaa cactaaataa
     9421 cattttgtaa aataaaaagg tgaaccttgt atattaggat tcaccttttt taaataaatc
     9481 aacattctgt tttaaaataa catttataag ctgttttgaa ataatgctta caaattattt
     9541 taaaacaata cctataaatt tttatttaaa gaatacacat aaatatttcc agactctttt
     9601 atcctataga ctttataatt attaaagaag tctaatctaa tttttgttaa aagtaattgt
     9661 aactttaaat aaatcaccat ccacacttat attcatctta ccaccctgta attctataaa
     9721 actttttgct atcgctaaac caagaccaga accttctgtg ttacgtgatt tatctcctct
     9781 tacaaatctc tccattatat catctacact aaaatcaatc tcagatgcag acatattttt
     9841 aagcataata tttactttat tatctgtttc taaaatatca atatacactc ttgaatttgg
     9901 cattgcatat ttgcttatat ttattataag attttcaaat actctaaatg tacgctgact
     9961 atctaatggt aatattattt tttcattaga aaagttattc tttactatta gagatgaatc
    10021 tgcaagtttg tcatctaatt ctaaaagtgt ctgcttcatt agagatacaa tatccacatt
    10081 tactatattc aaataaacat ctccactatt tgccttgctt acttcaaaca aatcttctat
    10141 tagatgttgc aatctttgtg actttctgtc taatgtatca atatacaact tacgattttc
    10201 atctgttata ctttcatctt ttaataaatc tatataagtg attatagatg ttagaggtgt
    10261 ttttaagtca tgtgatacat ttgatataag ctctgttttc atcttttgac tcttaacctc
    10321 ttcgtcaaca gcttttttaa atccagtttg aatatttcct aattcgtctt ttataggatt
    10381 aaaaaatcct aaatcttttt ccatatttac atctaagttt ccatctgaaa ttttctttgt
    10441 aacatctaaa agttgattat attcatatat tgtcttttta gagtaatctg acagtatttt
    10501 aaataatact actgaatata ttaatgcgag tattattcca aagaaccaca tactacacat
    10561 tataactata atcaataaat ttattcccaa aattgtaact atagttttat ttggcttttc
    10621 ttttagattt attgttctaa tttgattaat aactgttttt cctgcttttt ttagccatac
    10681 aaaaaacatt cctgtcacag tatgttttgt aaaatactct cttattccaa catttaaaat
    10741 atgttttaaa agtattataa atacaaatat tattgagaat attacaaacc aataagttaa
    10801 attcattaaa cttaccaata tactagccat tttagcactt atgtcattgt ttataaggta
    10861 ctctacataa ttcccactca aggtattact tattataaca gtaggatata aatctacaag
    10921 cccctgtata atagcaagag caataaagag tatctctaac ggtattcttg ataatgtatc
    10981 aaatcctagc aactctttag attttctata tgggaatgct aaagctacaa taacaactat
    11041 agctaatata atttttaaaa tcattccaga aatcttataa taagaattat tttctacatt
    11101 gcttatatat gaaattatac catcatcaaa ataagtattt ccataaccat actctacact
    11161 atcaccttta cttattactt taggtacagc atatactaat gttatatttt ttattggaga
    11221 taacttatat gaaattgaat ttcctcctaa acttgtatcc attaaatctt ctcttgcttt
    11281 agataacaac atttgattaa taattttttg atttactcca taagaatcca caatatttac
    11341 ttttccatct ttatcaaaat tcattacaac gtagaatcta tagttttctt ttatatctgt
    11401 aatctgcttt tgtctttcct catcattcat ttctgtattt tttgactgat tttcatcaga
    11461 attttctttt gaagcatcat tttttgttgt atcaagtact cctaattctt tattatcatt
    11521 aaaaaattca aaattgcttt ctttatcaac taaatagtac tttaaattct ttcctgacgc
    11581 tttaatgaaa tgctcccaat tttcaaagtt ttcattaaaa tctgcattaa tactatcatc
    11641 tgtatagtaa gtttctgaac catcataagt atctttttct ttacttacat tttttatata
    11701 gttcatagtt tcttcattga taaatagttt tgatggttgt atcatcttac cttcttgttc
    11761 ttccttcata ttatgatata aaacataact tcctttatat atactatcta caaagctttc
    11821 ctgttcaaat ggattctctg ctccaccttt agcacttgct tgtatagtag aatattgact
    11881 aatcatacct aatgatgctg ataacataag tataaaaact ataatagtaa ctatatattt
    11941 atctttaaat tttttcaatt ttgtatccaa caccccacac cacctttaaa tatttaggat
    12001 tttttgggtc aatttctatc ttttccctta tattcctcac atgtaccata actgtatctg
    12061 tatttacagc attctcattc caaactcttt catatatttc atcagcagaa aaaactcttc
    12121 ctgcatttct cataagtaaa cttagtattt taaattctat aggagttgtt tttatatact
    12181 ttccatcaat actaacttct tttgtatttt catttaattc taatccgcct attgcaaaca
    12241 cactatcgtc actttctacc ttttgctctt tatttccaac catgtttaga tacttagtgt
    12301 accttctaag ttgtgaatta actctagcca atagttctaa tggcttaaaa ggttttgtta
    12361 cataatcgtc tgctcctata ttaagcccca ttattttgtc aacttcctct gattttgcag
    12421 atagcattat tactggaaaa ttatagtttt ctctaagctt cattgtaagt gttataccat
    12481 ccattttagg catcattata tcaactatag ctaaatgtat ttcttggttt tctataacct
    12541 taagaccttc taatccattg tatcccttaa aaacattata tccttgattt aatagatata
    12601 tttctatagc atctgctatc tccttttcat cttctaccac caaaatatta tatgaattca
    12661 ttctaatctc ctttatatta atttataaat tttctttaca tatatgatta tacaatatga
    12721 tagaagtttt tccatttata tatatattta tatcttttgt tctggttatt tgtatttaat
    12781 gtccatctta gaaacctcct tctagtttaa atttaatatt ttttcatttt gttttatata
    12841 cttaatatta aaattaaaaa cttaagatat tacatatact tttctaaaga ttttctaaag
    12901 atttaaatat atttaaaatt aata
//
LOCUS       DAYASV010000106        12840 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYASV010000106 DAYASV010000000
VERSION     DAYASV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12840
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.107"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     regulatory      31..133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            227..1603
                     /locus_tag="ACGI68_002850"
     CDS             227..1603
                     /locus_tag="ACGI68_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFH0139277.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(1717..2862)
                     /locus_tag="ACGI68_002851"
     CDS             complement(1717..2862)
                     /locus_tag="ACGI68_002851"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0139278.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIILDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            3115..3822
                     /locus_tag="ACGI68_002852"
     CDS             3115..3822
                     /locus_tag="ACGI68_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139279.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            3869..5464
                     /locus_tag="ACGI68_002853"
     CDS             3869..5464
                     /locus_tag="ACGI68_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139280.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLMVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            5567..6649
                     /locus_tag="ACGI68_002854"
     CDS             5567..6649
                     /locus_tag="ACGI68_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HFH0139281.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            6798..6959
                     /locus_tag="ACGI68_002855"
     CDS             6798..6959
                     /locus_tag="ACGI68_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HFH0139282.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            7300..8568
                     /locus_tag="ACGI68_002856"
     CDS             7300..8568
                     /locus_tag="ACGI68_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0139283.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            8763..9551
                     /locus_tag="ACGI68_002857"
     CDS             8763..9551
                     /locus_tag="ACGI68_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0139284.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSIRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            9551..10000
                     /locus_tag="ACGI68_002858"
     CDS             9551..10000
                     /locus_tag="ACGI68_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFH0139285.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            10004..12268
                     /locus_tag="ACGI68_002859"
     CDS             10004..12268
                     /locus_tag="ACGI68_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0139286.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGSMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            12408..12719
                     /locus_tag="ACGI68_002860"
     CDS             12408..12719
                     /locus_tag="ACGI68_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139287.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         4703 a         1396 c         2187 g         4554 t
ORIGIN      
        1 tataaattaa aataatattc gtaaagaaaa attcatgttt tgtactgtat aacatataat
       61 tttgacaata tgggtcataa gtttctaccg gaataccgta aatattctga ctatgtatac
      121 tattatttag ttaattaaat atttaattaa ctgaattatg aaggatacgg ctcagcttat
      181 caactactgt gtcgtttttt tgtaaaattt aataggaggt gttgttatgc aaacaacaaa
      241 cagtagtact atgcagaaac tttttccaat tttgactaat aaaaatgtcg acatgaagaa
      301 ggaaataatt gcaggagtaa ctactttttt aactatggct tatataatag ctgtaaatcc
      361 aaatatttta tctgagacag gcatgcctgc aggtgcactt gttacaggta cttgtttagc
      421 agcagcattt ggatgtattt taatgggtgt agttgctaac cttccttttg ctttagcatc
      481 aggtatgggt cttaatgcat tttttgcata tacagtagtt ttacaaatgg gtgttccttg
      541 ggaagtagct cttacagcag tatttgtaga aggtattatt tttatagtat tatcagtaag
      601 tggagttcgt gaggctgttg taaatgcgat accaaaaaat atgaaattag ctgttacagg
      661 tggtatagga atatttattg cacttatagg tctagttaac agtggaattg ttattggaga
      721 ccaagccact cttatcaaaa tgggtagatt tacaccagca gtaataataa cttgtgtagg
      781 attaataatt attgctgtat tagataaaaa aagagttaaa ggttctatac tttttggaat
      841 agtagtgagt tcattacttg cttgggcatt tgcttttata aatccagagt atgcacaaaa
      901 attaggtata tatttacctg gtggattatt taaattcgaa agtttagcac caatagcagg
      961 taaaatagat ttaggatatg tactacatcc aacaaatata ggtggttttt tagtagtagt
     1021 ttgtacattt ttatttgttg atttttttga tacagtaggt acattagttg gtgtttgttc
     1081 taaagcaaac atgttagatg aaaaagggaa tgttcctaat gtaggtagag ccttacttac
     1141 agatgcggtt gcaactacag ttggtgcagg acttggagta tctacagtta caacttatgt
     1201 tgagagttca acaggagtta tcgcaggtgg aagaactgga tggacagcaa taacagttgg
     1261 aattttattc ttagctgcaa tgtttttctc tcctgtattt atagcaattc catcatgtgc
     1321 tacagcacca gctttaatat atgttggata tttaatgctt ggtacagtta aggatataga
     1381 gtttgataat ataactgaag gtgttccagc ttttgttact attgcttgta tggcattaac
     1441 atatagtata ggtgatggtt taactttagg tatattaact tatgttttcg taaatatatt
     1501 ttataatata tttggagcta aaaaggcaga agacaaaaaa catgtttctg tagtaatgat
     1561 tgttctggca atattattta taataaaact ttggctacta taataggtat tatttaaaat
     1621 tttaatttaa tataaaataa agggctgtaa tttattatca attgtgatga taaactacag
     1681 ctcttttgta tttgttgtat ttttgtattt attgtattat ttaataatgt ctattagttc
     1741 tccattttgt aaagttttag gtgtaattac tatattgaac tctttttgca aatcttttat
     1801 aatatctaat tctttttttg ttattattga gattgtgtta ccacttcgat ttcctctagc
     1861 agttcgacca catctatgca ggtattcatt tttacttttt ggaaaatcca agttaaaaac
     1921 atgacttaca tccacaatat ctagtccacg agcagataaa tcagttgtaa ttaaaatctt
     1981 tgctttacca agtttaaatt tatttattgc attctttcta tcttcttttt ccatatttcc
     2041 aaaaattcct attgctttgt aattatgata atttagtttt gatactagga cttctatatt
     2101 tttttcatta tttacaaata caatagcttt ttttggattt gtagctgcta atgcctttct
     2161 aagaaaagta aatttatccc ttatctcacc taataaatat gagtgattga tgttaggatt
     2221 tatttgtgat ttttcctttg tttttattat ttcaaactcc ttcataagtt tatcacaaat
     2281 ttttatagtg ctatcagtaa gacttgcaga gcatcctata atttgtctat ctcttaacgt
     2341 tgttcttatt atgtcttcta tacaagttat attttttcca ttcaatagat tgtccacttc
     2401 atctaagata atagttttta tattatgtga ttttagtttc ttttgtttta tcaagtctaa
     2461 aactcttcca caagagccaa ttactatgtg tggttttact gcttttatat tttttatttg
     2521 tttttgtata ttgacctccc caattaaagc taaagaagta acggagagtt ctgcattttt
     2581 agccaataat tcaacctggt ttgtaatctg cattactaat tcatgcgtag gtgcaagtat
     2641 aagagcttgt gtttctcttt ttgatgtatc aattttttca aatataggta aaaggtatgc
     2701 tagtgttttt cctgttccag tctgtgaatt aatcaataaa tccttatttt gaattatatt
     2761 ccctataaca agtgactgaa cttctgttgg agaagttatg tcttgttttt tgagtccatc
     2821 tattagagtg gaacttattt ttaattgttc aaatgtattc atattttact ccttatattt
     2881 taataattat agtcaactat tttttattat acattgttta ttcaaatcga actatatatt
     2941 cttttataat tatgtgtaat ttgaaagtaa atatcaatta tatttatacc tttatatttc
     3001 tgataccttt gcattttata gatttctaaa ataaacaaat aaaagttgta attgtttgat
     3061 atttgttata tattatatat aacaaatatt aaaactataa tatggaggga aattatgtta
     3121 aaagtaatca atgtatctaa atcatataaa aataaaaaag tagtaagtag tgtctcattt
     3181 gatgtaaatg atggagagct ttttgggttt ataggacata atggagcagg taaaactact
     3241 actattaaag caattgttgg aatacacgat tttgaagagg gagaggtact aataaattct
     3301 aaatctataa aaaaacagcc tgttgaatgt aaaaaggaaa tggcatatat acctgataat
     3361 ccagatttat acgagtcatt aactggtata caatacctta attttatagc tgacatattt
     3421 gaagttgcca aagataaaag agaagagtta ataaaatatt attcaaataa gtttgaactc
     3481 aataaagcat taggagattt aatatcttca tattcacatg gtatgaaaca aaaattagct
     3541 ataatttcgg ctttgataca cagcccaaaa atcttaattc ttgatgaacc ttttgtaggt
     3601 cttgacccaa aagcttcctt tatattgaag gaaattatga gggagttttg tgacaaaggt
     3661 ggttgtatat ttttctcaac acacgtttta gaagttgctg aaaaaatttg tgataagatt
     3721 gccataataa aagatggaaa tataattgct tatggaacta cagaagaagt taagggaagt
     3781 aattcacttg aaagtatctt tatggaatta atagagaaat aaaatgtttt ttatacaata
     3841 tagtgtctaa agtgaggaat gataaaatat gagcaatcta agtatattaa taaaaactaa
     3901 tataataaat gaatttaaat taaatgcgtt aaaaaaagct gatggtagag aaaaacaaaa
     3961 aataatggga atgatgcttt cagttgttat aatagcgggt ctattaattt tttatgtaac
     4021 aagcttaagt ctctctttag ttgatatctt aaagcaaata aatcaactgg aaatgctact
     4081 gatatttggt tttggattat caacattaac aactattgtt acatcattat ataaaacgtc
     4141 atcatatcta tttcaagcaa aagatttaga ccttttgact agtttaccta taaaagaatc
     4201 tactgtacta gcaagtaaga tattaatgtt ggttggagca aactatttat tttcttcaat
     4261 atgtatgatt atacctgcag tggtttactt tttaaattct gatgtaagta taacttattt
     4321 cccttatttg atattgctat ttttagcttt accattatta ccgatagttg cttcatcaat
     4381 aatttttcta tttatagggc agatatcatc aagaataaaa tacaaaaatt tagtattaat
     4441 cataggtagc attatattga tgttgcttta taccctactt atgtcaagac ttaattctat
     4501 gtcagttgaa atgctaaaga atagtgtgtc tatttcagag acaattagaa aagtatatcc
     4561 tcctatatat tattttgttg atgctcttaa aacaggaagt atagtttcat ttttagcatt
     4621 tatagccatc tcaataatac cattttttat gtttgtaact ctatttgcaa aaaaatttaa
     4681 agatataaat gctaagatga gtgaaggata taagtcaaaa aattatgaat ttaagggtca
     4741 aaaaacttca agaccttcaa aagctttatt aaaaaaagaa tttaagagat atacttcttc
     4801 gtttatatac tttttaaatt catctgcagg tttgttattt ctaatggtag gtacaatttt
     4861 aatattatta tttggagcag ataaagtggg tgagttactg aaattgaata tagattttaa
     4921 tcttataaaa atacaattat tgggagtatt cttctttata atagtaatga attgtacaac
     4981 atactgttca atttctcttg aaggaaaaaa cttatggata ctgaaatctt ctccaataga
     5041 tgaaagagaa atattctggt caaaaatatt gatgaatttt ttattgaatg caccactatc
     5101 tattttatgt ctaatattag tatctataag attagacttt gaaattcagt ttattattgt
     5161 atcaatctgt ttgattgtaa gtttttcaat gtttgtagca ttgatgggat tattatcaaa
     5221 tttattatac ccaaaccttg attggaaaaa tgaggtggct gtagtaaaaa gaagtgcaag
     5281 tatgatagtt acaatgttag tatcattagc ttatgtaatt atacttggag gtatatatgt
     5341 attacttgga atttcgtttt taaatatata tattattttg gcaagtatac ttactttgat
     5401 attagatttt atgatatgga gaattatttc tactaaagga gtaagaatat ttaaaaattt
     5461 ataggtgtat ttatgtcaaa taaagactta tcttttttag tcttgcaaat aataggttaa
     5521 gtagtatact atgtatagtg atatgaaaaa atttaggaga aaataaatga gaaaattatc
     5581 caaaagagaa aaagatttta ttcaatatgg atttttatta tttttgatat gtattactac
     5641 ttatttagta ttaactactt tagatataaa actaatacct aaaattataa ggctagtaaa
     5701 taagacttat atatttcttg gcatattgac aattatagta tatatgataa ttgaatctat
     5761 tgtaactcat ttgataataa atgcagttca aaaaacaaaa gtaaaattta ttggtattaa
     5821 aatggctaca atgggatttt actataactt agttactcca tttgcatctg ggagtcaacc
     5881 aatgcaaata tatgcactta caaaatatga tgtaagcctt agtaaatcag tggctatagt
     5941 tactaataaa actgtagtat ttcaaagtac agttactgta ttttgtggaa ttttaatttt
     6001 gctaaataag tcattactga tggagcaagt acattctgtg aaagtcttga taacttttgg
     6061 aatgattatg aatgtaagta tgttgttagg tggactacta atagtattta gtccaaaaca
     6121 agtaaaaatg atagcagaag ttttgattaa ttttttatct aaatttaaaa catttaaatt
     6181 tttagattca aaacgtgata gaataaatca ttatattgat gattataatt attctataaa
     6241 aatatttatt aaaaatgtaa gaatcttagt tttaagtata ttacttactg tgttacaatt
     6301 aatcgcatat ttcagtatag tttattgtgt gtataaagcc tcaaatttaa gaggagtttc
     6361 atatgtacat ataatgacat tacaggtctt tttatatatg gctatatcac caattccaac
     6421 accaggaaat gttggagcta atgaggttac attttttact atgttctcca atataattcc
     6481 aaaagaatta ttggggtact ctgtactctt atatagtggg tttgtatact attttatatt
     6541 aatagtttca ggtttattta catttggaac gcattataca ttaaattctt ggaaaaagga
     6601 aaataaagta aattctaatt tagataaggt tattgaaaaa ttaaattaat aaaataatta
     6661 gggaaattct atatataaat atggtgttga ataagctata ttatattgag atatttccct
     6721 tttttgatta aaattataga aatttgaaat aataagtaat aaacagaaaa agtgatattg
     6781 ttttgaaagg agaaactatg aaacaagata aacaaaatga aaaacaaagt gagatttcta
     6841 agataaaaca aagcaatgat aataaacttg aaatagctca agaattacat gtaagtaaaa
     6901 atatagggaa tatgcttgga tatagaatag ataagaaaaa aatgaataga ccaagttaat
     6961 gaaaatgttg aaaatggtac attttaaaaa atatatataa tgaaatgttt tattgttaaa
     7021 tttaaactta ttttaatggg ggaaaaagtc gaaaaatatc taaaaagata tggatataaa
     7081 aaatattaat atatatatac tataaacttg acaaaatcaa ccttttaata actagcaaaa
     7141 aatagcaaaa ttatataagc gttatttaat tgtcaaaaat gaaagctaaa ttgcatgtcc
     7201 taaaaaaaat gattgataac atttatttgt gtgctataat tatatagtaa aacaataagt
     7261 atattgcata atgtatgcaa atcgggagga gagacggaca tggaagcatc tatacacaca
     7321 attgccagca ataatttatt gataattttt gctattgtaa gtataactgg aataatttgt
     7381 agtaagctta gtgaaataat aaaggttcca gatgtagtgt tatatttact tgtaggtatt
     7441 ttaataggac catcattttt aaaatttata gatattcgtg gatttcaaat agaaaatcaa
     7501 ctaatactta catttggttc tgcatttatt ttatatttag gtggaaaaga aataagttta
     7561 aaagtattga gaaatgtgaa gatatctgta ttcttattag ctactttagg agttgtgata
     7621 tctgctttta taatgcaaca agtaatagga tttacttttg ggataagtgc aatgactgct
     7681 ttactagcag gaactataat agcatcaaca gacccagcca ctcttgtacc tatatttaat
     7741 caggtaaaga taaaggatag ggtaaaacaa actgtaataa gtgaatcagc ttttaatgat
     7801 gcaacaggag ctatactaac atcagcagtg ttggctgtaa ttttgtcaaa taaattttca
     7861 ttaggtgaaa atatttatga actggggaca atggttattg tcggagtttt agttggttta
     7921 attacaggaa tactgctttt gaaattagtt aatgataaac cttatggaat atttaaagat
     7981 tttgcaccaa taatatctgt tatttcagtt gtagttgcat atgaattatc tacaaaatta
     8041 ggtggaagtg gatatatgtc ttgctttatt gtaggtataa taactggtaa caagaaaaat
     8101 tttaaaattt ggttaagtca aaaatcttat gatgcagatt tttatgttgc tgaaacttta
     8161 ggtactattt gtcgtatggc aatatttata atattgggta gccaagttaa tttggctcag
     8221 ttaagtaaat attttatgcc atctgtaatt acagtattag tattaatgtt tatagcaaga
     8281 ccactttgcg ttttagtttg tacacttgta gacagggaag caaaatggag taaaagtgaa
     8341 atattattta tgatgtgggt aagggaaaca ggcgtaatac cagcagcact ttgtggtatt
     8401 atttcagcaa tgaaagttcc tggatacgaa gtgatttcat ctgtagtatt catgactata
     8461 ctaattacat tagtaataca aggaagtact acaaaattag tagctaaaaa acttggatta
     8521 ttagaagaag aagtgataaa tataagtgaa aaagtatcaa ctttttaaag ttttgcttta
     8581 aaatagtacc tgttaatggc tattttaata ttgtctaatg atgattattg aaatagtcat
     8641 ttttttatgt ataagtaatt tacagaattt acatattaga catatttatt atcaaaattt
     8701 gttgaagcta tgatataatt agttgtattt aaaataagaa tagcaaataa gggaggaaat
     8761 ttatgtttac ggttatggat attgtacagc cagatacagt agaagaagcg tactcaattt
     8821 taaataaaag aaaaactaac caagtaattg gtggaagtgc ttttttaaga atggggaaaa
     8881 aaagaatagg aacaggaata gaattatcta aattaaattt agattatata aaagagtatg
     8941 aagattacgt tgagattggt tctatgacta cttttagaac attagaaaca agttccataa
     9001 taaaaaataa ttttggaaga ataatagaag attcagttaa aaatataata ggagttcagt
     9061 ttagaaatgt agtaacagtt ggtgcaacag tattttctaa atatggtttt tctgacttga
     9121 tagtagcatt actttcatta gatacggaag tagaattata taatattggg agaattagtc
     9181 ttgaagagtt tctaaataga gattatgaga aagatttact tattaagata tacataaaga
     9241 agacaaataa gaatgcttct tataaatcaa ttagaaatgc aaagagtgat tatccaattc
     9301 taaatgtatc tgtatcaaaa catatggaac aatttaagtt atgtgtagga gcaagaccgc
     9361 agaaagctac gattgcaaaa caagctagtg aatttctatc taacaatgaa atcaatgaaa
     9421 ttaatataga taaagcagta gaaatagcat cagaagaact aacttttggt tctaacatga
     9481 gagcatctag ggagtataga aaagctatga gtaaagtact tttaaaaaga gctataatgg
     9541 aggttattta atgttagtaa agattaatat aaatgggaag aaaaaaagtg ttgatataga
     9601 accagaagaa tatctggttg atactttaag aaaagttggt aatttaagtg tgaaaagagg
     9661 ttgtgacaca ggttgttgtg gtttatgtac agttttaatt gacaagaaac ctacattatc
     9721 ttgtgcaaca ctgacattga gagctttaga taaagaaata actacaattg aaggtttaga
     9781 aaatgaagtt aaagaattta gtgaagtttt ggtaaaagaa ggagctgagc aatgtggttt
     9841 ctgttctcct ggctttatac ttactgtact tgcaatgaag gatgagcttg aaaatcctac
     9901 agatgatgaa ataaagcatt atcttacagg aaatctatgt agatgtacag ggtatatggg
     9961 acagttaaga gctatcaaaa cttatctgga ggtaaaatag aacatgaata tagttggaaa
    10021 aggaatcaag aaaatagatg gaatgagtat cacaacagga aagcctgtat atacagatga
    10081 tttatcagca aaagatgctt tggtggtaaa gatacttaga agtcctcacg cttatgcaaa
    10141 aataaaagat atagatacat caaaggcaga aatggttgat ggtgttgaat gtgttttaac
    10201 atataaagat gtaccaaaat ctcgatttac acttgctggt caaacatatc cagagccatc
    10261 tccatatgat agattaattc ttgaagatgt tgtaagatat gtaggtgacg aagtagctat
    10321 agtggcagct atagatgaaa aaactgcaat taaagcaatg aatcttatca aagtaaagta
    10381 tgaagtactt acacctgttt tggattttga agaggcaata ggaaataaaa ctatggtaca
    10441 tgatgaagat agacattgta actttgatat aggtatgcaa agagaaaaaa atatagtatc
    10501 ccatcatgat tatacaaaag gtgatgtaga aaaagtatta agtaattgtg atgtagtgat
    10561 tgaagaaact tattatactc aagctcaagc acatgcaatg atggagacgt atagggcata
    10621 caattattta gaccatatgg gaagattggt tgtagtaagt tcaactcaaa taccatttca
    10681 tgtaaaaaga catttatctc gtgcactaga aataccagct agcaaaatta gggttataaa
    10741 acctagaatt ggtggcggat ttggtggtaa acaaactgca tgtgttgaga ttttttctgc
    10801 aattgttact ttaaaaacag ggaaaccagc taaaataatt tatgatagaa aagaaacttt
    10861 taattgttca acaagtagac atgcaatgag attaaaagtt agactaggtg ctacaaatga
    10921 tggcataata aaggcaatag atataaaagc actttctgat acaggagcat atggagagca
    10981 tgcttctaca actattggtt tggttggaga aaaaacttta ccaatgtata ataaagttga
    11041 agcaacaaga tttattgcag atgtggtata tactaataag atgccagcag gagcacttag
    11101 agggtatgga gcatcacaag gatgttttgc tgttgaatca actataaata tgcttgctaa
    11161 taaattaaaa atggacccaa cagatttaag gttaaaaaat gtggtaacag aaggtgaaac
    11221 tacatttgca tatggtaaaa ctttaaatag tgtcgattta ataaaatgta taaataaagg
    11281 caaagaactt attggatgga atgaaaaata tccttgtaaa catcttgata atggaaaaat
    11341 tagaagtgtg ggtatggcaa ttactatgca aggttctggt atacaaggtc ttgataagtc
    11401 ttcagctgaa ataaaattga atgactgtgg agattatact cttatgatag gttctactga
    11461 tatgggtaca ggaagtgata caattttagc tcaaatggct gctgaggttc ttgatacaag
    11521 cattgaaaat ataacagtta tatcagctga tactgactta gttccatatg acccaggttc
    11581 atatgcttca agtactactt atgtaacagg tatggcagta gttaaggcag ctcaagactt
    11641 aagagaaaaa ataatttctg ctggagcaac taaactaggt gttgagaagg atgaagtaga
    11701 atttgatggt ataaaattaa ctagtaaaaa aggtagcact ttattattcg atatagcagt
    11761 tgattctaca attggtccag atactaatca attaataggt cttgcttctc atggtagccc
    11821 agtatctcct ccacctttta tagcaggttt tgcagaaact gaaattgaca cagaaactgg
    11881 aaaagttgat ttagtaaatt atgtagctgt agtagattgt ggaacggtta taaataaaaa
    11941 tctagctaag atacaagttg aaggtggaat tgttcaaggg attggaatgg cattatttga
    12001 agaagttaga tacacagaaa aaggaagcat ggacacaaat acatttatgc aatacaagat
    12061 tccagctaga tgtgatattg gaaatgttaa agtagatttt gttgaaactt atgaaccaac
    12121 tggaccattt ggtgctaaat ctgttggaga agttgttata aatacacctt taccagcaat
    12181 acaagaagct gtgtttaatg gaattggtgt aagaataaat acactcccta ttacacctga
    12241 aaaagttttt atggaaatga ataaataatt atttatgaaa atttagacta gttttacatg
    12301 ttaaacaacc agttatgtca tacctgttga aagatattta ataagtagtt attataatat
    12361 tatgaatttt acttataaaa tacaaaatta aataagggga tgatgttttg gctggtaatc
    12421 taaataatat gagagcagta aataatttta gaggagataa gaacatttta gaatgtttag
    12481 tcagctttga gggtcgttca ataagtcaga gaaaagtaag ggtatttttt aaagaaaaac
    12541 aaaatcaaat agaaattgat tttgcagaag aggaaatttc taaattggtt gaaaatgttg
    12601 ttttaaatac atcatatcaa gaaatgttat atgatgaaat agagaaacaa ctggaaattg
    12661 attgtatagg tacttggatg atattatcta aattaaaaga tggtagtaga gttcactgat
    12721 tttaaaataa attattagag cctaatttta aggctctttt ttgctgtttt taattaacaa
    12781 tagaaaattt atctttagtt tgtatataat tttaatatca tatattctat atatataaaa
//
LOCUS       DAYASV010000107        12777 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYASV010000107 DAYASV010000000
VERSION     DAYASV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12777
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.108"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(25..903)
                     /locus_tag="ACGI68_002861"
     CDS             complement(25..903)
                     /locus_tag="ACGI68_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0139288.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(920..1237)
                     /gene="trxA"
                     /locus_tag="ACGI68_002862"
     CDS             complement(920..1237)
                     /gene="trxA"
                     /locus_tag="ACGI68_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFH0139289.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1387..2619)
                     /locus_tag="ACGI68_002863"
     CDS             complement(1387..2619)
                     /locus_tag="ACGI68_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139290.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2609..3280)
                     /locus_tag="ACGI68_002864"
     CDS             complement(2609..3280)
                     /locus_tag="ACGI68_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139291.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLINQNIY
                     DLVILDLMLPNINGFDILKKMRINNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3296..4132)
                     /locus_tag="ACGI68_002865"
     CDS             complement(3296..4132)
                     /locus_tag="ACGI68_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139292.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4158..4832)
                     /locus_tag="ACGI68_002866"
     CDS             complement(4158..4832)
                     /locus_tag="ACGI68_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0139293.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4846..5574)
                     /locus_tag="ACGI68_002867"
     CDS             complement(4846..5574)
                     /locus_tag="ACGI68_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFH0139294.1"
                     /translation="MMFDRGKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAQDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5701..6390)
                     /locus_tag="ACGI68_002868"
     CDS             complement(5701..6390)
                     /locus_tag="ACGI68_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0139295.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6398..6961)
                     /locus_tag="ACGI68_002869"
     CDS             complement(6398..6961)
                     /locus_tag="ACGI68_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0139296.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6963..7931)
                     /locus_tag="ACGI68_002870"
     CDS             complement(6963..7931)
                     /locus_tag="ACGI68_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFH0139297.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8032..8075)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8441..9070
                     /locus_tag="ACGI68_002871"
     CDS             8441..9070
                     /locus_tag="ACGI68_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139298.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMHSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9045..9449
                     /locus_tag="ACGI68_002872"
     CDS             9045..9449
                     /locus_tag="ACGI68_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HFH0139299.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9452..9928
                     /locus_tag="ACGI68_002873"
     CDS             9452..9928
                     /locus_tag="ACGI68_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HFH0139300.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNNKIELS
                     KFDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPS
                     SLLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10500..11165
                     /locus_tag="ACGI68_002874"
     CDS             10500..11165
                     /locus_tag="ACGI68_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFH0139301.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLRQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11187..12119
                     /locus_tag="ACGI68_002875"
     CDS             11187..12119
                     /locus_tag="ACGI68_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFH0139302.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12140..>12777
                     /gene="pcp"
                     /locus_tag="ACGI68_002876"
     CDS             12140..>12777
                     /gene="pcp"
                     /locus_tag="ACGI68_002876"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFH0139303.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
BASE COUNT         4371 a         2036 c         1443 g         4927 t
ORIGIN      
        1 ttaaaaagtt aatcaatata aatattaagc cctctttaat ttatctaaat agtaaacagc
       61 atttaaagct gctatttgcc cttgaccagc tgcttttata taagaatagg gttttccaac
      121 acaatctcca gcagcaaagc atccatctat actagtcttc atattattgt caactttaat
      181 atgaatgcca tcaatctcta ttccaggaac caatgcactt ggagccgtac tatcttttat
      241 aacgaatact ccatcaactt caatgtggtt ttctttaaaa gatactttgt ttacaagttt
      301 atctccatct atctgtactg gtctgtcatg tataacttca atagaatcat ctaggttatc
      361 tgaacttctc ataagattat cttttttgta cataggtata aaataagtct ttgaagtgag
      421 ttcacttaaa aaatttgctt cttctttaga ttcttcatta tatcctataa ctgctacctt
      481 tttatttcta taaagtggtg catcacatgt agcacagtag cccacacctc ttcctagaaa
      541 ttcttcttca cccttgattg gtctagtata ctctacccca gttgcaagga ttacagtagt
      601 agcttcatac atatcatttc cagacattat tgtaaagtat tctcccatag cataaatatt
      661 atttattctt ttattttcaa ttgaaatatc catactatct atatgtgatt taaacttgtc
      721 ttttaattca tcaccactta tatcataaaa acctaaataa ttatctattg aaggagcttt
      781 aacaagctta ttacttaaat tatcattacc aaacatgata attgttttat ttcttatttt
      841 tgcatttata gcagcagaca atccagctgg accactgccg attatagcta tatcatatct
      901 cataatactc acccattttt tataaatatg attttacttg catttcaatt ttgttctttg
      961 gcatgaatcc aattaaagtt tctactggtt ttccatcttt aaatataatc atagttggta
     1021 ctgtagatac attgaattgt tgagctattt ctaaactctc atctatatca acttttacaa
     1081 atctagcttt atccttcatc tcttcaccta attctgcaaa tacaggtccc aacatgttac
     1141 aaggtccaca ccaagttgca aagaaatcaa ctacaactac tcccttacta ccttcaacac
     1201 tacttctaaa ttcagatgta tttataactt ttgccatttt taaatcctcc tattaaactt
     1261 tattatccct atataattat actatgaaat ttataaatat aaatttactt ttataaggtc
     1321 tttttatatt ttatactaat tagtataccc attcttcatt tctaaaaaca attttttata
     1381 tttttattat tttaactttt ttggtattga caattcaaat gtagttccaa tatctacatc
     1441 agaagttaca tttatatcaa tagataagtt taaactaatc atgcgagcta ttgaaagtcc
     1501 tattccagaa ccttctaaat ctttatttct tacattttca cttctaaaaa atctatcaaa
     1561 tataagaggt atctcttctt tttttatgcc tataccagta tcacttataa gtaaagttac
     1621 ctcatcctct atatcttctt tcaattctaa tgctatctca tcacccaatt ttgtatactt
     1681 aaaggcatta tcaatgaaaa ttaatataag ttgtcttaat ttatttttat ctgttattat
     1741 aactttattc ttcagtttag aattaaacac gaatcttttt tcctgaaact cagcaatgtc
     1801 aatatagtct tctgatattt cttccaataa cttttctaaa tctatttctt ctaaattaac
     1861 tttaacaata gagtcctctt tagtaagaga caataaatca gttatcattt ttttaagtct
     1921 tctagtttcc ttcattgcag tagctattgt ttcaacttca tcatttactg tactttctgg
     1981 ggattttaac atactttcta attttgatga aactattgtt attggtgttc ttaattcatg
     2041 agaagcatct tgtataaact tagcttgatt tttccatgct gtttctattg gaattaaagc
     2101 ttttctagtg agatatacag ccacaaagta agtaattatt acagaaatta atattccaat
     2161 tataagaaca gatgtaagct gtcttaaaga attcatctca gaatctatat ttctaattat
     2221 ctgtatttgg tatctaccta catctacatt taattctcta aaagtatatc cattttctgt
     2281 gtatttaaaa aacgaatttt ttttatctag tcttctatct ggaagtaact catcaaaata
     2341 ttcattttga gtatagtaac ttatccttcc atcttcatat acatacacca tatctttagg
     2401 atctttgagt cttattgggt tcaaaaatga agttctttta aattgagaga ctatatattc
     2461 aagttcatca tttatctcac tatcaattcc actatatgta agtcccctaa aatatgagta
     2521 tataaatata gaaaagatta ttaaaaaact acctactaca ataatataca tttttattaa
     2581 atgattttta gtactggtaa atacattctt atttatcatc gaatatatat cctaccttac
     2641 gtttagtttt aatatattta tcatatccat atttacttag cttttttctt agattactta
     2701 catatacttc cactatttca atagttgcat ctgaatcaat tccccatatt ctatcataaa
     2761 tctgctcttt taataaaatt gaacctttat ttaatacaaa atactcaagc aagttaaact
     2821 gtttgttttg aagttttatt tcttcttttt caatatacac tcttttttct agtgtatcta
     2881 aatataaaga tttaaattct aatccgtttc tttcttttat ctttccatta gtccttaata
     2941 tagcgtatac cctagctact aactcttcca tataaaaagg ttttgtcaaa taatcattag
     3001 ctcctatagt aaatgcttct actttgtcat ctaatgtttc ttttgctgtc aatataagta
     3061 caggtgtatc tatattatta attctcattt tctttaggat atcaaatcca tttatattag
     3121 gaagcattaa gtctaaaatg actaaatcat atatattttg atttattaag tataaagctt
     3181 cttctccatc ttcacacttt tcagtttcaa aatgcttgct aagttcttct actacacttt
     3241 ctaaaagtat cttattatct tcaactatta aaaccttcat aagtatcccc tacctttatt
     3301 tgtctatctt aactttatga gattttaagt caatatccac attggctttt tcttttatat
     3361 catcatacgt atatcccact atttcaacac tttttgcctt tgttgaacac aatatatcta
     3421 tatctgtctc ttctgaaggc tttaaaatct tatatgatgg tacacgattt actgttaaat
     3481 attctccatt ttcattaact tctgctgtct taagaataat atttcctaaa tcttttgatg
     3541 atgaattttt tacttttact tgatacgtaa ttccctcatt tgattcattt acttttttaa
     3601 gttctgatgt agatagcacc tcatactgac tactattttc tagcttaata ttattatttc
     3661 ttatattaat agtattttct tttaaattaa catacacaat cttaccctct gcctcatatt
     3721 catatgcata aacttctaca ccctttacaa aagttttatg cccacattct atgtaagcaa
     3781 ctttcccagg cattaaagtc atgtccaaca aagccttact atccgaagat ataatttttt
     3841 tattctcatc atattcatta tatatcaatt caacattgga tatattaaat gttgaattat
     3901 tttctacctt tgttgatgta tctactttat caccttcatc tatatttata atttctgatt
     3961 ttcctatatt caatactttt gcatttccct catctatttt tatctcattt ttatcttctg
     4021 tttgaatatc aactgaatca ggctgttcat ctttttcaca tgctgtcaac ataaaaataa
     4081 atattgttaa tataatcatt agagaaattg atttttttac tattttattc atattaagca
     4141 cctcatatag tagtatttta tagtagattt ttatactttt tcataaattc tttcaatgcc
     4201 tgtgtaagta tttgatgttt ttcaaaattc gaattactac ataatctttc aaaatcattc
     4261 catatttctt tatctatacg tattcttgtt gtttttaatt catctatgtg ttcatcatct
     4321 atatgtacat cttttttatt agatactttt ttaggcttta tattgtaatc ttttacttct
     4381 aaataccaat catatacttc acaaagctta tctaaatctt cttgagttat atttattttt
     4441 ctatctttct taggcttaat attttgtttt ggctttgcat tttttaaacc tgtatcatta
     4501 aactcaattt gttcaacact tttacttaaa gccttttcat tagaaatctt ttttttggtg
     4561 cttacttttt ttctattact tatagtattc tttttacttt tttcatactc cttagctttc
     4621 tcttcttcca agtttctatt atccaaactt ttattataat ttaattcgtc ttttaatatg
     4681 tatttacctt tttctgactt atatcctttt ttatttaaaa aagttcttaa agtactttga
     4741 ctaatcttta gattttgagc tatctctgca aatttaaatc ctttgctttg aagttctaaa
     4801 aatttgtcaa ttctgtcttc attgttattc attaatattc ctccgtcaac atcttcttgt
     4861 ctttcttttc atacttctta ttatattctt cttttctaaa tctactctat ttgattcaat
     4921 tattttttgt agcattttat tataagtgaa atcatctaaa ttattatttt ttatatagag
     4981 aaaagtttcc tcttcacatt taataaaaca aattgatagc aaccatgcca tacccatttt
     5041 aacataatat tcttcacttt gtatattatt acagtattca aatatttctt taatatgttt
     5101 atcatcaaca taatatatta aaaacataac taatgcaaat ctaatttccc atggattctt
     5161 tgaataaaca tattttttaa gtatatcata catattttct ttatattttt tcatagactt
     5221 taaatcagaa caaaatccat cgcaaactga ccaattatcg attttaggta taaatctttt
     5281 tacatagtat aaagtttctt caaaattact atttatattg ccaattacca taccttgtag
     5341 cctaacttct tcatagtatg tatcctgtgc tacttctaaa aattccttcc aattcccttg
     5401 gcttatttct tttgataact ttcttaaatt aggaactcta atacctatta tattttctgt
     5461 tccaggtata agctttttat taaaatctct atattttata tcttgtatag acatcatata
     5521 ttctaaaagt tcattataat ccaatttatt ccattttccc ctatcaaaca tcatttaatt
     5581 gctccttaat tccttttttc ttattgtaac ataaaactct acaaaaaaag cttctaacta
     5641 ttagaagctt tttcaaaaac ataagaaact tttcaatttt aaatttaaac taaattaatg
     5701 ctaatctcct acaccaaagt ctgtattatt gtataaaact tctatatttg gattttcatc
     5761 caagaattta tacttactaa caaaccattt cactaactct tcatctcttt ttatactaga
     5821 agaattattt ataaaaacat tcactgtcgc tttttcaaaa gtatttagga taatctctat
     5881 atctttatct atcatctctt ttgtttgtcc ttttattcca accataatac aaggtgaatc
     5941 aaaatattct tttacatctt gtggagtctt aaaatttgca tttttattta aaatattatt
     6001 tctaaaatca ttgtcaaagg tttcaactcc aattttaaat accacaggaa tttcaaaata
     6061 ctctctcatt tccttaagcc tatttttgta agaccaatga ctttctaaaa ataatttttt
     6121 tatattcttc tcttttataa tacactttat tttttctaaa gtatccttag gtaattcaaa
     6181 acagcttcca gaatttatga cttcaagaac tccatatttc ccagttacgt tttttaaaac
     6241 ctttagattt aatttattca tctcttcttc taaattagaa ttatcatcta tataatcaca
     6301 aaaagaacac tttccccata tacaaggaaa tcctttaagc agaacaattt ctctttgatt
     6361 tttatttgtt atctcactat atctatccat tttctatcta ttttcctcca gtcaatgata
     6421 cctttaaagt ttttggaact gcatttaatc ctaaattcac taaaacaact gctaacagct
     6481 tatctccata atcagtaaat acttgggtta caaacacact aaaaacatcc cccagtccta
     6541 acactttcaa gatttgcact atatatgatg aacctgaaga ggtcatacct ccaaataaaa
     6601 atgctgaaat tattgaactt attatagatg ttggaatggc aaataaaagt actcctagtg
     6661 gagtttttaa accccttaaa aaattctttt taaatgctat tccagcaagt aatccggttg
     6721 atatttgaac tggtgcaaaa tagagagaat acacatcaaa cgtaattcca cttattaaac
     6781 ttccaaacac acctgtaaca acagcgtatt taggtcctaa aagtgaagct atcattatag
     6841 ttcctattga atctaagtat acaggtaatc ttaatcccat agctataaaa gcgcctatta
     6901 tatttataga aataccaaaa gctataagtg taatagtttt tacacatatt tttcttttca
     6961 cactatatca ctccttccac agagtctata atgtcttcat aacctttaaa aagcctcttt
     7021 aaaaacatgt acataaattt cttctcatca acttcattta gaacaattgc attaggattt
     7081 tttttataga aattgaaaga atccactata gactgaccca tagctattcc atcttctaca
     7141 acttctacat atgattcaaa tcctttacat atacttctat ctataaagta tgctactgct
     7201 agagggtcat ttatcacaca gccaattatt ccctcctgtt cccaatggaa atcaatataa
     7261 aatctagtta tttcagttat atactttgcc atcttcttat caagtctatt tataaactca
     7321 ataatattag gagtaagtac aatttttcta gttacatcta aacctaccat gtggattttt
     7381 ttagataaat tcttgtaaac ataatctgct ccatgtgggt ctacccaata attaaactct
     7441 gctactggag agcaatttcc atgaatccta aatgctcctc ccatagatac aaactcatcg
     7501 agattttcaa atgctttctt atctttaata agtgctttag ctatatttgt aagtggtgca
     7561 agtgctatta ttgatacttt ttcatgatta tataaagtat ttattataaa atccactgct
     7621 ccattttttg ctttagctcc tacaaccttt tgataaaagt tttctccaat accatcttct
     7681 ccatgtgtat cttgagctgt tacaagtttt ctttttagtg gtgcttcttc tcctatatat
     7741 actggaatat ttagtgaaga acacatttga agtgttttta gtgcattttc tgcacctata
     7801 tttgctggga catttccaca acatgtggta attccaatta cttctagctc tggtgagttt
     7861 aaagccagaa gaattgccaa agaatcatca attcctgggt cacaatcaat tattactttt
     7921 ctcttttcca ttttttatcc tccttgattt gtggtcgagg agaactacac atcttttttc
     7981 cactttcaaa aaggtgtgtc taagggtatt aattatatac tgccactata taattcctta
     8041 gttttttata cttggagttc tcgaacaagt acagatgtaa aatcatattt ttaggctact
     8101 tactataaga ctcttaaaaa ctatagaccg ttaaaaaatt tttaaacatc tgatttaata
     8161 gctataaaat aaattttata gcataaaaga atttattata ttttaatgta tcttattaaa
     8221 gtttatttag aattatgaac caactttaat aagatgtggc gcatttaaac cgattattat
     8281 tatacaataa tatcttaaat attacaatta ataacattaa tttattatcc ataaaaatat
     8341 attaacttat attatttaat aatttattga cagtattaaa attaaattat acaataacag
     8401 tgtaaattaa cttaacaccg ttaagcaaag aggtgattat atgagtatca aagaaagacg
     8461 taaaaatgaa aaagaagaaa tgaagaaaaa gattatggat gctagcatcg aaattataaa
     8521 ccaacatgga tatgaaaatt tatcaataag aaaaattgct acaaaaatag aatattctcc
     8581 aactacaatt tatctttact acaaagacaa agcagagatt atcagcgata tgaccaataa
     8641 actttataac acagtagaaa gtaatgccat tgacattatg aataattgct catcactacc
     8701 aatagataaa caaatcatag aaattatgac tagctttata aaaactcttt ctagtgaacc
     8761 agagatggca aaatctatca tgcatagtag aatgaatatt atatttgcat ctgaaaatac
     8821 aaacaatacg cctagcaata atggtataaa aatgcttgat aaattccttt ccattggaat
     8881 tgaacaagga atattcaaaa aaaatataga taaaagctca tggatgctta taagtgcatt
     8941 gttaggattc gttttatgtg ttgttgaaaa taaactatat tctttacata atttttctca
     9001 acttatagaa aatttcttag atattttagt gggaggactt tacaatgaaa attcgcaaca
     9061 aggctactaa taaaaagaaa aaatatgtaa aaatatctat tgctctattc tttgtattta
     9121 tatgcattat tattgtaatt gtaagcaact taaagccaga aaacttggta gtaaaagata
     9181 tagatattaa taatgttaaa aatggcattt atactggaag tgctgataat aacttggtaa
     9241 aagctactgt gtctgttgaa gttaataatg gtaaaatcca aaatattaat atcttaaaac
     9301 acgaccatct tttaggtaaa cctgctgaaa aaattacaac aagtattatt aaacaacagt
     9361 ctttagatgt ggatgctata acttctgcaa cttatagcag taatactata cgaaaagctg
     9421 tagaaaatgc tttaagaaag ggagaataat tatgaatact attattatat attcaagtaa
     9481 atatggttgt acaaaagact gtgcaaatat actaaaaaat aaactatcag ataatgtcac
     9541 tattgttgat attaataata ataataataa aattgagtta tcaaaatttg ataaaattat
     9601 tattggaagt tctatatatg ttggttcagt ttcaaaaaaa atacaggtgc tatgtaatga
     9661 taatgtagag ttattaaaca aaaaacaagt tggcattttc ctttgctgtg gcttttctga
     9721 acaagcagat aaatatttaa aatcaaattt tccatcatcc ttattagaaa gtgcaaatgc
     9781 tattggaatt tttggtagtg aagctcgatt agaaaaaatg aaatttttag ataaacttat
     9841 tatgaaggct gtttcaaaag gtaattatga cagttttaga atatcacagg ataatataga
     9901 taatttttta ataaatctta atagctaaaa taaagctaat tatttctatg ataaattctt
     9961 ttatttataa gtaatattgc tcaaaatagt ctttaaactt tatcaaaatt ttatttttct
    10021 tagtgaaatc taatgaaact agcacttaat atatgcttta ttaatcataa caaatatgtt
    10081 ttattaatca taacaaaaag agagtgtcta atgaactaaa aaattcatta gacactctct
    10141 ttttgtatga aatcaaatat atttttatca tttcaacaga gaaaaataca cttgtctcta
    10201 tgtttggcac attctctttt tataatataa tgtttaattt aaaactacta tttcaaatat
    10261 tgacatatac ttaaactata gacttacaaa atcaattcat attagcttaa aatataatct
    10321 ttgttattca aattcctata cacaaccatt atttactttg atactttaaa acatcttccc
    10381 tattttttat taaattttat aataatagtc ctattaacgc aaatactata aaaagagttt
    10441 aactttatcc attaaataaa tttaaattaa aacatataga aatggaagga gaacaattta
    10501 tgataaaact aatcggtata ttgatagttg taattggatt tatacttaaa attgacacac
    10561 tatttactgt attattagca ggagtggcaa caggaattgt tgcaggatta gattttaatc
    10621 aaatttttac aattttagga gattcttttg tctcaaacag aggtgtatca ttattcatat
    10681 taacattgcc agtaatcggt gtattagaaa gatatggttt aagacaaaga gctgtttctt
    10741 taatagaaaa attaaaaaga cttacaacag gaaaagtact tactatatac atgatagcta
    10801 gacaaatagc tggtgctcta tcaataagaa tgagtggaca tcctcaattt gtaagaccac
    10861 ttgttaatcc tatggcacaa gctgctggtt taagtaatag tgatgaacta aaagaatcgg
    10921 atgaagaagc cataaaggca ttatctgctg catcagaaaa ctatggaaat ttttatggtc
    10981 aaaacttatt tgcaggtagt agtggagtat tacttatagc atctactctg actcaatttg
    11041 gatacaatgt aactggactt aatatcgtaa aggctagtac tattatggca gtaatagcac
    11101 ttgtagtggc tacaatccag tttaccttat atgataaaaa attaaacaaa agtcataaaa
    11161 aatagtttta tttaggagga ataaaaatgc aaaattttat aaacatatca ttggaaatct
    11221 tttacgcact aatgggattt ttaatgattg ttatagcata taagtctttt actaccataa
    11281 ataacaataa gaaatatggt acatcattat tttggatact tatatcactg ccattcatat
    11341 ttggtagatt gattccagcc aatataatag gaataatatt aatattatct agtttattaa
    11401 cactttctaa acaagttgta tttgcaaagt atgaagaacc agatgaaaat tttggaaaag
    11461 aacaagcaga caaacttaaa aataaaatat ttataccatc attaatatta gcatttgctg
    11521 cagtagtagt agctatgagt ctttctaatt ttgataattc ttctcagttt gcaataggtg
    11581 tgggttcaat aattgcatta atatcagccc ttataataac taaggcaaaa cctgctacat
    11641 cagttcaaga tggctcaaga ttacttcaac aaatgggacc tgctagtatg ttaccacaac
    11701 ttttagttgc attgggagct ttatttactc aagccggagt cggtgaagtt atttcaacca
    11761 tgatatctgg tgtagtccct gctgatagca gattatttgg tgttatagct tatgtattag
    11821 gtatggttat atttaccatg ataatgggaa atgcatttgc cgcattttca gtcataacgg
    11881 caggaattgg tattcctttt gtattgtctc aaggtggaaa tcctgctata attggagcat
    11941 tagcattaac agcaggatat tgtggaactc tacttactcc aatggctgct aattttaata
    12001 tagttccagc agccttgcta gaatgtaaaa atgattatat tgttataaaa tatcaagctc
    12061 cagttgcttt ggtattaatt atagcccaca tattagtaat gtacttttta ggattttaat
    12121 ataattagga ggtaaaataa tgaaaatttt gttaacagga tttgacccat ttggcggaga
    12181 accaataaac ccagctcaag aagcagttga aagggtcaat aataatataa acggtgcaga
    12241 aatcataaag ataactatac caacagttat gacaaaatct gttgaagcta tagataaagc
    12301 tattcaagag cacaatccag atatagtaat atcagttgga caagctggag gtagatttga
    12361 cattacacct gagagagttg ctataaatat agatgatttt agaataaaag ataatgaggg
    12421 taaccaggtt atagatacta ttataaaaga agatggtgaa cctgcatatt tttcaaaatt
    12481 gcctgtaaaa gctatggtaa aacatatgaa tgaaaataaa attccagcta gtgtttcaaa
    12541 cactgcagga acatttgttt gcaatcatgt gatgtatgga attctttata tgatagacaa
    12601 gaaatatcct aatataaggg gtggatttat acacatccca tatacaactt cacaagtaat
    12661 agataaaaaa aatacaccat tcatgtcttt agaagaaata gtaaaaggct tagaattagc
    12721 tatagaagct tgtattatat ataaagaaga tgtaaaagaa attggtggtg aaatttc
//
LOCUS       DAYASV010000108        12718 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYASV010000108 DAYASV010000000
VERSION     DAYASV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.109"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(107..1987)
                     /locus_tag="ACGI68_002877"
     CDS             complement(107..1987)
                     /locus_tag="ACGI68_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HFH0139304.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRAE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKVTINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGSGGNNGGGSNSGGSSSGGSSGGGNSSDSTSNVTVKKLKGDDRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNDKLPQKTVEELK
                     RLNPSKVVVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2197..8160)
                     /locus_tag="ACGI68_002878"
     CDS             complement(2197..8160)
                     /locus_tag="ACGI68_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HFH0139305.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSDSESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDEQQ
                     ENSDKQEVNSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSKSTLE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVTYPAGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDDLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELARYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQGISKGNADLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRIEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDANTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGTMNNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8284..8369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8541..9944)
                     /locus_tag="ACGI68_002879"
     CDS             complement(8541..9944)
                     /locus_tag="ACGI68_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HFH0139306.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            complement(10406..12301)
                     /locus_tag="ACGI68_002880"
     CDS             complement(10406..12301)
                     /locus_tag="ACGI68_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HFH0139307.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKGGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT         3531 a         2344 c         1407 g         5436 t
ORIGIN      
        1 aacctcaata ttattagatt gttaagataa tgaaaataaa ataagaatgc atttaaatat
       61 ataactttat atctaaatac attcttattt tctatttact taatatttat ttttcaatta
      121 aactcttaat cttgtctatt acagatgagg ctattcctcc acctacttca aacaactttg
      181 atgaattttt accttttaat gacgattctt gtgatttatc taacgtattt gtagcaagta
      241 taactggtga attgttttta gctgctaatg gtccaactgc taaagcatct ataagtttat
      301 cttgatttgc aactacaact ccatctagct tagattgaga gtagaattta tctattactt
      361 tggcattcgt ctcttgtcta gtttgacctg ccaccctatt gtttgatata tctttgtttg
      421 ctatttttcc aacttgttca atagccttgt tagatatttt ttctactcca ccaataaaat
      481 atatatttga caccttattt gatttaataa attgttcagc ttcattatct acaccatctt
      541 tttgtgtaag tactattgga gttttttctt taccagccag tgatgctatt gataatgaat
      601 cagcttctcc atttcctgct cctatataca acttgcttac attattcgtc ttatctattt
      661 cttttgctat attaatagaa gtttgatatc ttgtatctcc acctattctt tgtactgata
      721 tttttgaatt tacagctttg atagcttcaa caactgaatt tggcattgag ttgtttccac
      781 ctatcacaac tacttttgat ggatttagcc ttttcaattc ttctacagtt ttttgaggaa
      841 gtttatcatt tgatgataaa agaatcggtg agttttttac actagcaagt ggtgtagctg
      901 taagcccatc caccatactt ttatcttctc catttactat tacaactgta tctgattttg
      961 tccaaccact ttgagatatt ttgatagctg tctcaaatct atcatcacct tttaattttt
     1021 tgactgtgac attagatgta gaatcacttg aatttcctcc tccagagctt ccaccactag
     1081 atgagccgcc tgaattactt cctccaccat tatttccacc actaccatta tctggtggta
     1141 attcatccgg ttttgataat tcattttcaa ctatgtattc taaacttttt ggaagtgtaa
     1201 cttcaacata tttgtttatt gtaacttttc ttcccttagt tgttttttgt gctaatccaa
     1261 gttttaatat tacatttgaa ccactttctt gtgtttcttt actaacaata gaatagttta
     1321 tcctaatatt gtttaataaa tattcaagtg cttcactttt ctctacctta tacttttcag
     1381 ggtcattttt actagaacca gttcccccaa tcggttttac ttcactctca ttaataaggg
     1441 taaatattaa tccttcatca gagcttatat tttcataatc cactgttttg ttgttatatg
     1501 ttacaatttt taaagctggt ttatcatttg ttccttttcc taaaacttta tagtcagcat
     1561 ttgagttact tccagatttt tttacagata ttcttcctat attcaatcta tttccgtcta
     1621 gtaagtcttc atttgatgta acatatatat ctaatgtagt tgtattactt gatttgttgt
     1681 attctgctct acaagtagca tttgcatttc cactttttat ctgagatttt atattatcag
     1741 accaagtaat cttttctttc tgctcattat ctactacttc aacctcacct tgtactttta
     1801 tactggcttg aaatgaatat actatgtttt ctactgagtc cacagatata tctatagtat
     1861 ttactttctc atacgttttt gttccttgta aaactggagc tgcatatgca catgtagcac
     1921 taccagttac agctactgcc atagttgata ctgctatagt tcttataaaa gattttcttt
     1981 tactcattaa cacccgtcct tatctatcat taactaagtg gataaattta cttatccacc
     2041 tagcttttat atttaattta taaattaccc taaatttatg attacattta tatactttac
     2101 ttttaatatc tactatttta aaactattct agtacctaat cttattagat atacaatgtt
     2161 agtgttagta tttttacgtt tttagagttc cacctactat ttatctctaa caatattacc
     2221 ctttacttta ttgcttacag ttataacctt cttattactg atatcaatag aatttcttgt
     2281 agcttttcta gctttactat tactatcagt aagacttatt gtctttaact catcataatt
     2341 cttagctcct acaggtacat acattgtatc acttacaaat ctttgaccta ctggagtatc
     2401 tccttgaagg tcattgtatc tatatagttc agccttaacc ttatcactta aagaatcaaa
     2461 gttttctttt ggcatatagt aaacccatgt acctttactg atttcaccta gttcaccttt
     2521 ttctggaact tctgcaaata aaagtgcttc tccagatata gtcttattgt ttttatctat
     2581 aagtagtggt aaattaaatg ctggatttcc actgtattca cctgtaataa ctattgaacc
     2641 cgctggaatc ttacctgttt catcttcact tgtgctagag tcatcacttt gatatacata
     2701 atcttctgct aattttccaa ttccattagt acgagtttcg tctgcattat tcattgtgcc
     2761 aatttctata ttatcacctg gaggtcctat tatatctaat tctgcaactt ctatattaga
     2821 cttattattt gagattagtt ttacatatct agcttggtat gtccaaagtt taccatcttt
     2881 attaaatgta actctagctt cattatcttg gtcttttcct ccaccaacta aatcttgtcc
     2941 atattcaaaa gttccttgac ttacatcttc ccagttcttt ttatcattac ttacttgtat
     3001 tttgtaatta gttaaaggat tgtatgttgt gctagtattc tttctaaagt taaataatcc
     3061 ttttaatgag aacttcctag cttctgtctc tggttttgta tattttagtc ctactatagg
     3121 taatgtttct ttcatgtcta ctattatata tggattttcc tctacattta tatcagggtc
     3181 tttttgaggg tctttgttcc actctgcttt acttattatc tttcctttat atgtagtatt
     3241 tgcatcatta tcttttatat tatcaatttc aggattttgt aatggtccat gtgagtcatt
     3301 ttcagttcta acatcaccgt cagaaacagt atttgttatg aatgaccatg ctgatttatt
     3361 tattttccct tcatctgata attttacagt accaacttca aattcttctg ttggatttag
     3421 gtgataatca tatgcaacta ccttatatgt aaatgctctg ttattaagag gagatatatt
     3481 atcagtaaat ttagtcttac tatttccttt tgcatccaca aatccaactg ttctgtattt
     3541 tacctgtgtt ccattttcac catctaaata atttccatca cttcttatta tttcgtatcc
     3601 tagtatctta tctttatcct tatctacatc taggtctata gatattttat tagatttaac
     3661 tagagaacca ctttgtattc catcagaaaa actagcactt actttagtat cttttgccat
     3721 actagctata ttttcattta aaatctttct tctagcttcg tcatttacat attgtatctt
     3781 tcttgattct ttttcgtagt tcttactatc cattatactg tttaattctg gtgtaatttc
     3841 tactccccat gccttaaagt aatctcttaa atctttttca acaacacttg atgccattgc
     3901 tactagcatt tggtcttttg gcaaactatt aattgggtca ctacttgaaa gtgctctgta
     3961 ttttcttgaa atctttgcat agaatgtatc attacttaaa tcagcatctg agttattctt
     4021 taacattgaa cccgtaaaat catcatcata agctagatgt aattgccata acattccaag
     4081 cctttcaaat acattgcttg gtatacctaa gctatttgaa gttacatgct catatatctt
     4141 atccattgtt gaaccttcaa ttcttgaatg tgtcttatca tcaaaggtct gagcaagtaa
     4201 agcaagtaca ttgttactag tttcgctata tgtcatcttt ccaatatctg taacatgtcc
     4261 tatctcatga cctattcccc atccaaataa gtttcctcct gtagcttttc cactatcatc
     4321 tatttggaaa ggtactccat tcataagccc tggaactgaa ccatattcta ctcctacatg
     4381 taatccagaa gcatacataa atgctccaat aaacattctt tggtatttta tgttcatacg
     4441 agattttgga gccatatcat ttttagttaa gttaaattca ttatcatcta cagttccatt
     4501 accatctaaa tcttgtgttt taaatactcc tctttctgag taaactaaat ccattatttg
     4561 ttcccatgca agcatactat tatataatct atctacttgt gtatttaaat cagcatttcc
     4621 ttttgatatc ccttgatata cagctgttgc aggaacattt aatgttactt tatcactttc
     4681 aatatctgta gtgtttaaca caccagtttt ttctgaatat ccatatgaat taccaacatt
     4741 tgttcctgga taatatggtt ctccttttac agcatcttta tatctagcta attcttctat
     4801 atatgctttt atctttttct tagattcaga aactcccgca ggactcttat catttattgt
     4861 tccatttaga ttaaggtgtg gtatcttagt agctcctgct acacgtactt ttatatctcc
     4921 tccacttgga gtatcttcag cgtatcttat gtataaactt cctccacatt ctatatctaa
     4981 actatgaagt ttgcttaatt gaattacatt ttttccagtg ttaagattta tttctccact
     5041 cttatatgca ccagattctc catagtactg tgtatatatc aacttaggag tttttccact
     5101 tttacttcca acatatactg tgatttcatc tcctgctcta gcagctactc ctaatgcctg
     5161 ccagtcattt tgcataccta aattattgga atttacagta tactgtccgt ttttatcttt
     5221 atattttcct aatttattta tttcttgatt tacagtagtt acatcagtaa gtgtggctct
     5281 atcattatat aagtcttgag ctaattttaa ttctctctca ataactgtct tatatggatg
     5341 atattctcca ctagctttat ctggagtatt taatctatct tttaattcag ttattttttc
     5401 ttgagttaca tcttctttta attcaacttg taggtcatcc ataaatagat ttcttacatc
     5461 atcttctata ctgtcatact cataaaactt aagttctgat atattagact tatatgaata
     5521 ggatgaacaa acacttactt gtatcttggt agcagttatt ggttctttta atctaaccat
     5581 tacatactta ttattattta cagggtctgt ctttgtttca aaaggaacat cccccagatt
     5641 gtgtaattga ccattttcat cccaatatcg tacctcaaag tcatttattg tcttatcaga
     5701 aacatcaaaa cgaggtgtag ccataaaagt atccatagta taagatttat caaaagttat
     5761 tattggtcct ctattcgttc cctcagctgc attatatgtt gggaatgtcc aatgtgttgt
     5821 aaagtcccca tctactacat tgaatttatc aaatggattt ttatcactat attcactttt
     5881 gtcatatcct gctggataag taacatctac aatgtgattt gttggtattc caaatccacc
     5941 attctcttta ggagtattaa ttaatctata gttaggtgtt gttggtgcat ccatgctttc
     6001 agttgtacct atatatgttt tagacattcc actagttccc aagtggttag tagctgtcat
     6061 tctaacttca tatgttgtat tatccttaag accttctatc tttgaacttg tcccacgaat
     6121 aagcttatca ttgtatttta tccaagaccc attagagcct ttttctttat agtacaagtc
     6181 aaaatcttta gcttgtttat gatttttcca gcttacatca agagatttaa atttaccttt
     6241 aacatttata ccttctggtg gttctggttt tgtgtctgga acaacttgaa attctaatat
     6301 actatctggg ctaaattgta taggtttata gtttgaatct acattttctg gtactccgtc
     6361 taagttttct ggtgttgcat ctttataacc actagaccaa tctccattta aagattgtat
     6421 gctaacccta tatagtccat atgcttctat atctctaact tctattgtat ttttactggt
     6481 ttgtaatttt ttagtagata atacttttcc cttactatca attttatcta gtataagttc
     6541 gtatgctgat acatttactt cattattcca ataaaaagat actttttcat ttccaacagc
     6601 agtgctagtc gatattttat ttattttagg tatattcatc tctggctttg gtatttcttt
     6661 atatacattg tttaaaaatt caacttttga tatttctgca agatttctat cgtttgctgt
     6721 tgtagctgtt atttttatag ttatcttttt tactgctact tgtttaccaa gatttattac
     6781 tatatcacta ttttgaacat ctgcatatgt ttctaatgtt gacttactat atgtagatgt
     6841 tgtagagtta tcatctttac tgatattacc ctcttctttt ggagtatctt catctgaatt
     6901 aggtttatcc attgatgaat tttctttatc attattttta tcttgattgg tattagagtt
     6961 tacttcttgt ttatcgctat tttcctgttg ttcatcatta ttttcatctt tattatttga
     7021 gttattatca gtctggttta tattttcatc tttattattt acttcttctt gatttacttc
     7081 ttcttgttcg ttatttactt cctcttgttg ctcctcatct tttaagtttt tcatatattc
     7141 tttgttttca taaggaatct gttgaacatt accatctttg tcagtaacct caactattcc
     7201 ttttgctgga gcagtacctt ctggagcttt tatattgatg acttccatat taagtgcttc
     7261 tgataaagct atatccatta ctgctggttt ttcctcagat atttcatcat tttttgaaga
     7321 tattgtaact ccattatttt ctccactaaa taaattttct acattaccat taatatctac
     7381 atcactattt ccttttaatt caaccttact tgtatctact ataggattgg tagatactat
     7441 actttctagg ctttttgcat cttcactttg tcctaaattt ttctgtacat aatctaagtc
     7501 aacaatatct acctttccat ctctatttaa atcaaattct cttttatttg aatctatatt
     7561 ctctaaaacc ttattataat cactcttatc tactttaccg tctccattta catcacccat
     7621 taagaacaat tcataattta aaggtttatt tttattatct acacttgctc tattgcttaa
     7681 taagactctt tgtgaataac ttgatatatc aatgtcttta atttctttgt tcttaaatcc
     7741 atctccagag acttctacat tgtattttcc aacaggtaaa ttattaaata caatattata
     7801 atatgatata ttctcatctt tattttttat ttcacttcta cttgcagata atttttttat
     7861 agtatattct atattatctg tcccaacatt tactgttcct gaatcttttt ctccaagctt
     7921 tattattcca ctattgttac tatcatcttt taatcgtaac tgcatattag tcatactttc
     7981 tgaatcgcta ttttttattg gcagtgaaaa atttaaatct acttcaagac tcccccttaa
     8041 cttttctgta actgattttt caactgccct tagcttcccc tctaatatag gttctgcaaa
     8101 aacgtttagt ggtgcactac acatagatac tgctagtaca caagatattg cctttttcat
     8161 gaaataacaa tcctccctta ataaatatct aatttttcat gaatttaaat actataagtt
     8221 tcagacaata aaaaaagaca ctcaaagaga atcttaatta taaaataaaa agactttact
     8281 ctttttagca actggctgat atggctttaa aagctttaat ccctatagtt ttgcgtcatt
     8341 agatttccct aattttgcca tttaattttc actactttct tacccatttt atactatatt
     8401 taaacttttt aatacaattt ttttagatta aattataaaa aaataacatt tatattaatt
     8461 tattaatata aatgttattt tccattataa tttaattatt tatatagctt ccttacaagc
     8521 ataattttgt gtatattttc ttattcttta tatcttccat aaaattcatg tgtagcatat
     8581 agattcttat ctcctgttat agctatacca aatcccatta tttcataccc tttatgcaac
     8641 atgttagttc cttcttctgg atttgtcttc cactcattaa aaataaagtt tcctatatca
     8701 tctgcatctc tatttgtata tacaggttta tctggaattt tatatttaat tatattttca
     8761 ccttgaactg ctatatatcc aggtttaatc tcactccaat ctaagtattt caaaaaagta
     8821 gaatatgaat tttttccatt tatcgtatga gataaagtct tcttatttgc cattaaatta
     8881 gaccaatcat ttgctaaacc ctctaatctg cttgctacag ataacgctct tactccattc
     8941 tcttttctat actgctttgt aagagcatat actttaccct ttattctctc tttaaatgct
     9001 ctatcatttg gattgtaata taaaccttgt tgatttggtg aagcttgatt tatacattgc
     9061 ttgataacat tctgatttat gcctcctagt acagtcaatt tgtctgcacc acttaaaata
     9121 ctcttgttac tgccattttc aactaaaact acaggagcat tttttgctat tgtagaggca
     9181 gccaaagcgt ctgttaattg taagccttta cttagataaa attctttact atttttataa
     9241 aactcttgta taactatctt attagtttca aatctatctg tgccacccaa tctaatagaa
     9301 tttgtgctct taactaattc gtcgctgatt atttcactag aaccaagcgc ataactcttt
     9361 aagcctgtcg tgtcaaatcc cacactattt ccatctgtaa gtattatagg tactccatct
     9421 ctagcagcta ctggtgatgc acttatcgca tctgcttctc ctgtaaatcc attggctata
     9481 attactttat ctattggctt aatttctttg attttttcag ctatttttat acttgtatca
     9541 aatctatttt caccaaagat tctttgtaca accttctttt tggatagtaa ctgattctca
     9601 acatttttac ttattgttga ttctgagcct attatataca cagtctttgc tttgtttaat
     9661 ctttccatag ttgtgtttgg aatctgattt tgttttgtca tcaaaatagg cgcatttaag
     9721 gctccagcta aaccacttgc acttagacca tctgctaagc tattatctga gttgattagt
     9781 attgctgtat tgtagttttg cttatctgct atttttgctg ctgtctcata tctgtcattc
     9841 ccttttattt gctcaactga tgtcaacgca tttaccatag gagtatttac cattactaaa
     9901 aatagtgtaa gtcctatagc taatatcttt ttactattct tcatattatt ttctaccccc
     9961 aatgtacttt tcatattttt tattagtact catacttatt tttaatcaaa agttacaatt
    10021 ttgttaccat attttatata tgataccata gttatccata caactcaaac aatataccta
    10081 aagtttttat tctctataaa ttcaaaacat tataatttat tttatttaat taagttataa
    10141 gttcaaaata atttatgtta ataagatgtt taatcattat aagaagtatc tttttataat
    10201 aatttttgca atatgaattt ctcaacatta taatcaaaaa ataaaataag gtatctcaat
    10261 ctaattctaa atgagacacc ttatttttat tattttatct tgttatttgt atattttata
    10321 atatctacac tatgtcttac atattaacgc attgtataat catatattat ctaaacaaac
    10381 ttttaatatt aattcctata tcttattagt tcttagtaaa taagtttatt ttttctgcta
    10441 ttttttcaat agcgttttta gctattcctt cacctatttg tactaactta gtagcattct
    10501 taagtcttaa ctcaactact tcttcttgtg atttagaaag ttcttctgta gttaatacta
    10561 ttggagcctt tgaactagct gcaagtggtg ctgctgtaag tgcatcaact aattgtccat
    10621 ttccacctac ataaccatct tttgctacat atgctttagt tgcacctgca tagtatttat
    10681 tgattacttt agcattagta tcttttctat tttctccagc taatctttca actttattat
    10741 ttaaatctat atcaactaat ttgtcttcaa cttcttttga tacagaattt tctccaccca
    10801 ctatttctaa ttcttttcct tttaataatt tttcagcatt tgcatctaat tcgttacctg
    10861 taactattat tggagcacca aattgagcag ctctagctga tatactcata gcatcagctt
    10921 ctccatttcc acctactaca aaagcagtct taaatgtatc ttttaattgt tttgctattt
    10981 ctaaagaagt ttcaaatcta tcttctccag aaactctctc aactttactt acacctaatt
    11041 tagaaagttt ttcttcagtt tcttttgata ctttagattc tccacctact atatatattt
    11101 tcttagaaga tagattactt ccaagtactc ttaatatttc agcttctatt tctgatggta
    11161 gtgaatcttt tttagataat aatattggtg catttttttg agaggcaagt ggtgctgatg
    11221 ctaatccatc aactatagca tcttctccaa ctaaaattat agcttctgct tctccatctt
    11281 tgaatctctc tttactaact tctactgctg ttgtaaatct tgaatcacca gataaaactt
    11341 ctacttttaa ttcttttcca ccttttacat cagttaatac tttgtgtaag tctctaacta
    11401 acttatggtt tttacttgaa acacttattg tttctggctt ttcatttgcc ttgcttaaaa
    11461 ctatatcaaa gcttgatttt tctggtttat ctttatcatc actatcttct tgttcttgag
    11521 taaggattgc ttcattttca aatgttaaag catatccatt tttaacttta ttaactattt
    11581 ctcttcctaa tgtagttaat ctaattccat caaataattc ttctgctgat atgtcttgta
    11641 aatctgcgtt tttaactgtt atcgtatgta attcttttga atctatgtca ctataactct
    11701 tttcataacc ttcaaaagta ccttcttctg atgttatcac ttttgagaaa tctaaatgtt
    11761 ggtctcctac atttaatgta ataacatttg ctgaatcttt tgctcttttt acttcaactg
    11821 tatttgtaga ttttatagtt gctgttaact tgttatcaga atcttctgct aaagttgcat
    11881 tgtaagcatt aactgcatct actatttctt gagcagtttt gtagttttca attttatagt
    11941 caactacttt tccacctaac acttgatgtc ctttatcttg aatagtaact tttaaacttt
    12001 gcccttctgt taaagagttt atcttatttt gtaattgagt tgcattagtt aagtttacat
    12061 tgtctacttt tatatcgtaa actctatcat ttacatttgc tcctgctttt gtatcttcaa
    12121 acttaacttc taatgctttt ctaacctctt cgattagttt agcagaatct ttcgcactta
    12181 ctgtgtaatt tttatcaaca ttttctgtca cagtattgtc tgcgaatatt ggtgctgctg
    12241 cactcataac tgtagcacct gccattacta ttgccatact tctctttttg ttgcttaaca
    12301 tatttatgtc ctccgaatat tattttttat tttttatatt cgtatttccc acacataaat
    12361 aatattatat ataatattat ttttcaatat attttatgtg tttacataat ttactaacac
    12421 aaacactaat aatatcttct tgaacccctt ccccctgctt agctctctat aattatgctc
    12481 aaagatagaa atcctatttt atagtgattt tctactaaac aatcatatac taacaaaagt
    12541 attcaaactc catttttata tactactatg cactaatttt tgctttactc ttattactta
    12601 aatatatatc actaaattaa tatctatctc catatattaa aatatataac atattataaa
    12661 aatcatcata aattagagtt taaaattaaa aattattcta atacatgtaa ttgataaa
//
LOCUS       DAYASV010000109        12381 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYASV010000109 DAYASV010000000
VERSION     DAYASV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.110"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..321
                     /locus_tag="ACGI68_002881"
     CDS             <1..321
                     /locus_tag="ACGI68_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0139308.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKS
                     VYKK"
     gene            337..1920
                     /locus_tag="ACGI68_002882"
     CDS             337..1920
                     /locus_tag="ACGI68_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139309.1"
                     /translation="MRILIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKNLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI68_002883"
     CDS             1948..3276
                     /gene="ssnA"
                     /locus_tag="ACGI68_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFH0139310.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3475..4827
                     /locus_tag="ACGI68_002884"
     CDS             3475..4827
                     /locus_tag="ACGI68_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFH0139311.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            5019..6128
                     /locus_tag="ACGI68_002885"
     CDS             5019..6128
                     /locus_tag="ACGI68_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0139312.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI68_002886"
     CDS             6411..7802
                     /gene="hydA"
                     /locus_tag="ACGI68_002886"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFH0139313.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI68_002887"
     CDS             7796..10357
                     /gene="xdh"
                     /locus_tag="ACGI68_002887"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0046415 - urate metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HFH0139314.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSIPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI68_002888"
     CDS             10509..11855
                     /gene="rpoN"
                     /locus_tag="ACGI68_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFH0139315.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         4947 a         1428 c         2509 g         3497 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggctatatg atgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctacgcaggt
      181 gaggctggaa taagaactat aggatttggt ccttctaagg aaaatcttgc tcatacaata
      241 gatgagtata tagaattaga ccaattattc aaaggtgcag aaggatatta tggtatatta
      301 aaatcagttt ataaaaaata attaggagat ggttttgtga gaattttaat aaaacatggg
      361 cttatagtag atggaaataa aacaccagca tatgaaggag atattttaat agaaaacgag
      421 aaaatattaa aaatatcaca agatttaaac gaagaagctg acaaagttat agatgcaaaa
      481 ggtagagtta tatgtccagg tttcatagat actcatagcc attcagacct agttatttta
      541 gttaatccat ataatgaggt aaagattaga cagggaatta caacagaagt gttgggccaa
      601 gatggtatat ctatggcccc tctacctcaa gagcatatat cttcttggag aaaaaattta
      661 gcaggtctag atggtgaaag tgatgaaatt gattggaaat atgaaacaac agagaattat
      721 ttaaaaatga tggattacaa tggggtaggt ttaaatgaaa cttatttagt acctcatgga
      781 aacgttagaa tggaagctat gggacttgaa gatagacctg ctactaaaga agaaattcaa
      841 aaaatgtgtg aaatcacaga gagagaatta aaagcaggag ctataggatt gtctactgga
      901 cttatatata taccttgtgc atactcattg actgaagaaa ttatagagat gtgtaaggta
      961 gtagcaaaat atgatggagt atttgtagtt caccaaagaa gtgaagcaga tacaatttta
     1021 acttcaatgg aagaaatcat agaaattgga aaacaatcag gagtaaaagt tcatttttca
     1081 cattttaagg tttgtggtaa agcaaactgg aagtatatac cacaagttat agagctttta
     1141 gaaaaagcag aaaaggaagg aataagagta tcttttgacc aatatccata tgcagcagga
     1201 agtacaatgc ttggagtagt tttacctcct tgggcacatt ctggaggaac tgataagtta
     1261 atagaaagat taagtgatga aaacgaaaga gcaaaaatga aaaaagatat agcaaatgga
     1321 atagaaggct gggataattt tatagaattt gctggaatag accaaatatt tgtaacaagc
     1381 gtaaaaacag aaaagaataa agatacaatt ggtaagaact tattagaaat tggaaaaatg
     1441 agaggaaaag acccattaga tgcaacgttt gatttattaa aagaagaaga aaatgcagtt
     1501 gggatggtag atttctatgg attagaagaa catataatag gattcatgaa aagagacgag
     1561 caaaatgtgt gtacagatgg tcttttagca ggtaaaccac atccaagagc ttatggttct
     1621 ttccctaaaa tactaggaag atatgttaga gagttgaatg tgttaacaat cgaagaagct
     1681 gtttataaaa tgactaaaaa ggcagctaca agctttagta taaaagatag aggcgaatta
     1741 aaagaaggat attttgcaga tatagttata tttgacaagg atacagttag tggttgtgat
     1801 gattttatca actctatgca gtaccctaca ggtatagatt acgtaattat aaatggaaat
     1861 tgtgttgttg aagaaggaaa atacaataaa attaaagcag gaaaagtact taaaaattaa
     1921 atcttagctt taaggaggaa taaatttatg attttagtgg ggaatgggag gctaataagc
     1981 caagattctt taaatcccta tatggaagat ggatgtgtag taataaatga taatctaatt
     2041 gaagatatag gtactacaga agaaatgaaa gctaaatatc cagtacatga atttatagat
     2101 gcaggcaaga aaataatcat gccgggtcta ataaattcac atatgcatat atacagctca
     2161 tttgctagag gaatggcagt tccaggaaaa ccttctgaga actttttaga gatactaaac
     2221 aatttatggt ggagacttga caaacagctt acactggaag atacaaaata cagtgcatat
     2281 gcaacgtata tagaatgtat aaaaaatggt gtaacaacag tttatgacca tcatgcaagt
     2341 ccaaatgcaa ttgaaggcag tttgtttaca atatcagatg ttgcaaagga tttgggaatt
     2401 agaacgtgtc tttgttatga agtgtctgat agagatggtg gaaatacaat ccaacaaggt
     2461 atcaatgaaa acgtaaactt tataaaatac gctcaaaaag atgatacaga catgataaaa
     2521 ggaatgtttg ggcttcatgc tgcatttaca ctatctgatg atacattaaa taaatgtgct
     2581 tcagagatgg ctggtcttga tacaggttac catgtacatg ttgctgaagg actagcagac
     2641 ttacaatata atgtagataa atatggaaaa agggttttag aaagattcaa tgattttgga
     2701 gtactaaaag aaaaaacaat agcagttcat tgtatatata taagtgcaga agaaatcagt
     2761 cttttaaata aaacaaatac atttgtagtt cataatccag aatctaatat gggaaatgca
     2821 gttggatgtt ctcctgcaat agagttatta aaaaggggag ttacagtagg attaggaact
     2881 gatggatata ctagcgatat gtttgaatca ttaaaagttg ccaatataat tcacaaacat
     2941 cacttaaaag acccaagagt tggatttgaa gaatcacaaa caatgttatt tgataacaat
     3001 agaaaaatag ctaaagctta ttataaaaat gacctaggag tacttaaaaa aggtgcttat
     3061 gcagatgtta tagtagttga ttatatacca catactccat tgacagaaaa tacaataggg
     3121 ggacacacta tttttggaat gagtggaaga agcgtagata caactattat aaatggaaag
     3181 gtacttatga aagatagaaa gttaataaac atagatgaag aaagtattct agcaaaaagt
     3241 agagaattaa gtcaaaatct atggaataga atttaaataa ttgattatgc aaaattctat
     3301 gaagcaggaa ccctgtgact ttagtcatgg gaggttcaga ttaatataca agtaagaaac
     3361 aagaatttag atgttaaaaa cagagcttaa acaaaaatag gaatttaaat gaagtatata
     3421 aataaaaaat atacggaata gaatattaaa ttaaatatag aagggagacc caaaatgtct
     3481 cgagataaaa gccctaaaat agctccagta gatgaaaggt tacctgtatc caaatcttgg
     3541 gtattttcac tacagcacgt tttagcaatg tgtgcaggag cagtagcagt tcctatgatg
     3601 ataggaagtg ctgcacgact agatcatgct agtattgttt ttttaataaa tgcttgcttg
     3661 tttatggcag gtataggaac actcatacaa agtgttggta taggaaatat tgtaggtgct
     3721 aaaataccag taatagaagg aactagtttt gccgctgtgg caggtatgac agcaatagcc
     3781 tcatcatata gtgattcaca agttgcaata cagacagtat ttggctctgt aatagtagct
     3841 ggattgtttt gtatattact tgctccagta tttgaaaaat taataagatt tttccctagt
     3901 gtagttactg gaacagttgt tatggtaata ggtatttcac tatttccagt tgggataaaa
     3961 tggataactg acaacaaatt ggaagcggtt gaaccaaact cattagcatt agctttagca
     4021 gtattggcaa taactttact ttcatttaaa ttcttaaaag gaatttggaa tagcacagct
     4081 atattggtag gtatagtact tggtacacta ctttccatgg cagttggaat ggcagatttt
     4141 agtgcagtaa atggagttgg ttggcttaac ttaaatgtac ctttaaaatt tggaatgcct
     4201 agatttgaac catctgcaat actatcattg tgtttgatta tgttggtact tatgacggaa
     4261 tcagttggta atatgatagc tattcatgaa atggtagaca aagaagtaac aggtaaaaat
     4321 attaaaagag gtctatttgg agatggaata tctacagcat tatctggaat attcaattca
     4381 tttccaataa ctccatttgg tcaaaataca ggaatagtag gtcttacagg tataaaatca
     4441 agatttatag cagtttacgc aggggttata ttgctagtac ttggattctt accaaaattt
     4501 gcagcaataa taggagcaat acctaagcct gttcttggag gcgtaggatt tgcaatgttt
     4561 ggaatggtaa tggttggagg agtaaaaact ttaagcaaag tgaaatttga tggaactaaa
     4621 aatggggtta tagtagcagt tagtgttggt ttgtctatga taccactaac aaatcctgat
     4681 ttctatcata atttcccaac ttgggtacaa acaatattcc atagtggaat aacaacaggt
     4741 agtttatcag cagtgctttt aaacatattc ttcaatgaac taggttctaa aaaagaaaaa
     4801 aataataaag ttgaagaagc aaaataaaaa taaaacaagt aaaaatctat attgacttaa
     4861 agtcattaag tattaataaa aataattcag agatattaat aattcaacaa aactatctta
     4921 tattttaaat aatataaaaa agtaaataaa taacaaatac aaataaaaga atacaatcta
     4981 aatattaaaa atatttaatt taagggagag ataataatat ggctaattta aaagttaatt
     5041 tatatggaat ggaattttta aatccaatta tgacagcggc aggacctgga gcaaaagatg
     5101 gtgctctctg tcaagaggca gcaaaaggtg gagcaggagc tatatgtaca aaaactatat
     5161 ctgtattacc agctgatgta ccaagaccat gtatggcaaa aactaatagt ggttttttaa
     5221 acacagaatt atggtcagag cttccaaaag agcaatggat agaggaagaa tacaaaaagg
     5281 taaaagaaac agggcttcca gtaatagtaa gtatgggata ttcagcagag caaatagctg
     5341 aggttgctcc tttagtaaag ccttatgcag atgcagttga attatctact cattatgtag
     5401 gtacagacgt tgcaccaata gtaaatgctt taaaagctgc aaaagctgtt ttagatgtac
     5461 cagtatttat gaaaatgagt cctcatactg acattcaaac tattgcaaaa gctgttgaag
     5521 aagcaggtgc agatggactt gtaatgataa actcatttgg accatgtatg gcaatagatg
     5581 taaatacagg ataccctata atgggaagta aagctggata cggatggtta tcaggtgcac
     5641 caataagacc tctagcaata agatgtatat atgaagcttc tcaagttgta aatataccaa
     5701 taatcggtgt tggtggaatc acttgtggaa aagacgtggc tgaaatgatg atggctggag
     5761 caagtgctgt tcaagtatgt acagaagcaa tccttaaagg gccttctatc tatggaaaaa
     5821 ttgctaaaga attaaatgaa tttttagatg agaatggata tgctgatgtt aatgaaataa
     5881 aaggactaac tcataagaaa atggcagcta gagggtttag aactcattca gtagctccag
     5941 tagtggatga taacaagtgt atcaaatgta agcaatgtgt aacaagctgt gtatacgaag
     6001 ctttagaagt tacagataag ttgaacatag atgcagacaa gtgttttgga tgtggattat
     6061 gtgtaactag atgtccaaaa ggagctttaa gcatctgcta caatcaagta gaagctatga
     6121 atgagtaata attatataga attttggtaa ttaacttaga gtaataggta ttaggataat
     6181 aaaatatatc agataaacaa acacacatta tgcattaata atacagtcta aagttaataa
     6241 ttttattata ttgatttaac taaatattag tttagctgaa tattaattta attaggtatt
     6301 ggtttaatct taatatagat ttactgcgta taagtgaact aactagatta ctttaagtta
     6361 attttttcat ataaaataaa ttgatacatt acaagtaagg tggagtctat atgggaattg
     6421 ttttaaaagg tgggaaagta gtaacaagtg aaaaaactta ctgtgcagat gtaagaatag
     6481 aagatgaaaa aatagtcgat ataggtcttg atatatcaaa agaaaatgat gaaatagttg
     6541 atgttagtga ttcttatgtt ataccaggag gtatagatac acacacacac tttgacttag
     6601 atacaggcgt tactaagaca gcagataact ttgagacagg aacaaaagct gctatagtag
     6661 gaggaactac tactatattg gattttgcta cacaagctaa aggatgttct ttgaaagacg
     6721 gattagaaga atggcatgaa aaagctaagg ataaatccta ttcagattat ggctttcata
     6781 tggctataac agattggaat gactcggttt gtaatgaaat ggaggatatg gtgaaagagg
     6841 gagtatcttc atttaaactg tatatggcgt ataaaggaag ccttcaagtt gatgatggtg
     6901 taatctttga agcattgaga aaagcagaag agataggagg gattattggt ttccattgtg
     6961 aaaatgggga cataatatgt gaacttgtgg ataaagcaaa gtcagaaaat aatttatctc
     7021 caaaatatca tcaattaact agaccagcta taatggaggc agaagctaca tcaagactta
     7081 tgaagatagc tgaagttgca aactctaaag tatatgtagt tcacttaagc tgtaaagaat
     7141 ccttagagga ggttttaaaa gctaagcaaa gaggtgtgga tgtaatagtt gaaacttgtc
     7201 ctcaatacct tctattaaat gatgaattat atgatttaga tggatttgaa agtgctaagt
     7261 atgtaatgtc accaccttta agaaataaag aaaataatga aatattatgg aatgctattt
     7321 tagataatca aatagacact ataggaacag accattgttc ttttaattat aagggacaga
     7381 aggaacttgg gataaacgat ttcagtaaga taccaaatgg ttctccaggt gtagaacata
     7441 ggatgagtct tttatacaca tatggagtct gtaaaaataa aatatctatc aacaagatgg
     7501 tagaagtaac atcaactaat gcagcaaagt tgtttggaat gtatcctaat aaaggaacta
     7561 tagaaatagg cagtgatgca gatatattag tcttaaatcc aaacaaaaaa tccaaaatag
     7621 aagcaaaaaa acaattacaa aatgtagatt atacacctta tgaagggtat gaagtggatt
     7681 gtcaaataga acatgtttat ttaagaggaa aagaagtagt tagagatgga gaattaattt
     7741 gtaatactcc aagtgggaga tatataaata ggcgtctaac aaagggggct agagaatggt
     7801 agaatttata ttaaatggag agaaagttgt tgcaaaagaa ggcaaaaaac ttttagactt
     7861 ccttagagaa gatatgaata taacttcagt taaaaatgga tgttcagaag gtgcttgtgg
     7921 aacatgtatg gttataatag atggtaaagc tactaaagca tgtgtattta aaactgataa
     7981 attagctggg aaaaacataa caactataga gggattatca gagagagaaa gagatgtttt
     8041 tgcatattct tttgctagtc aaggagctgt acaatgtgga ttttgtattc caggtatggt
     8101 aattagttca aaagcactta tagacgttaa taataatcct actgaagatg atataaagaa
     8161 agctttaaaa ggtaatatat gtagatgtac agggtatgta aaaataatta aagctgtcga
     8221 tttagcggca aagattttta gagaaaatat agatgtgcca aaagtaaact gtaagggtct
     8281 agtaggtgaa aatcttccta gagttgatgc agttggcaaa gcacttggat attctgaata
     8341 tgtagatgat atgagaattg agggtatgat ttatggttca gcagttagag ctaagtatcc
     8401 tagagcttta gttaaaaaaa tagatataga agaagcaaaa gcttatccag gagttgttgc
     8461 agttctaaca gctaaagata ttccaggaac tataaaggta gggcacttaa agaaggattg
     8521 ggatacttta ataccagaag gtgaaataac tagatactta ggagatggaa tagttttagt
     8581 agcagcagaa actagagaag cgttagaaga ggccaaaaag ctagttaaga tagattatga
     8641 ggaattaaca ccattaagta ctacagcaga tgcacttaga gaagatgctc caaagataca
     8701 tgagacaggg aatatacttg ttaaggaaca tttagtaaga ggaaacgcta aagaaaagat
     8761 agaaaattca aaatatgttg taactaaaag atatactaca cctgctacag aacacgcatt
     8821 tttagagcct gaatgtgcag tagctggtcc ttatgaagat gatggagtaa taatctattc
     8881 gacagaccaa ggggtgtttg ccactcaaca tgaatgtgca gatatgttag ggttaccata
     8941 tgagaaggta agagttataa acaaaatggt aggaggaggg tttggtggaa aagaagacat
     9001 gagtgttcaa caccatgcag ccttacttgc ataccataca aagagatttg taaaagtaca
     9061 gttaacaaga aaagaaagta ttattgttca tccaaaacga catgccacag aaatggaatt
     9121 tacgacagca tgtgatgaaa atggatactt aacgggtatg aaggcaacga taatatctga
     9181 cacaggtgca tatgcctctc ttggaggacc agtgctacaa agactgtgta ctcatgcagc
     9241 aggaccatac aattatcaag atattgatat aacaggaaca gcagtatata ctaataaccc
     9301 tcctggagga gcatttagag gatttggagt tacacagagt tgttttgcaa ctgaatgtaa
     9361 tctaaattta cttgctgaaa tggttggaat atctccttgg gagataagat ataaaaatgc
     9421 aataagacct gggcaagttt taccaaatgg tcaatttgca gatgaaggaa cagcaatggc
     9481 agagacacta gaagctgtaa aagaagagta tgaaaagcat ccacgtgcag gtatagcttg
     9541 tgctatgaaa aatagtgggg ttggagttgg tatacctgat gttggaagat gtagattggt
     9601 gataaaagat ggcaaagttc acataagaac aagtgcttct tgtataggtc aagggcttgg
     9661 tactgttatg actcaagtag tttgtgaaac tgctaaccta cttcctgaaa atatagttca
     9721 tgaagttcca gatactcatt taacaccaga ctctggtaat actacggctt caagacagac
     9781 agtatttacg ggagaagcta ctaaggttgc atcaaataag ttaaaagaag ctttagactc
     9841 tggcaaaact ttacttgatt tagagggaga agagttttat ggagaatatg aaagtattac
     9901 agataaaatg ggttgtgaca aagaaaatcc agtaagtcat gttgcttatg gatatgcaac
     9961 acaagttgta attctggatg aaaatggaaa actagaaaaa gtagtagcag cacatgatgt
    10021 tggaagagct ataaatccaa tcagtttaga aggtcaaata gaaggtggag tagttatggg
    10081 tcttggatat gctctaaccg aagattaccc agtagttaac tctattccta cagctaagtt
    10141 tggtacatta ggtttattta aatctacaga tataccagaa atacaagctt taatagttga
    10201 aaagaacaaa gatttattgg cttatggggc aaaaggtgta ggagaaattt gtacagttcc
    10261 tactgcacca gcagtacaaa atgcctatta tgtatttgat aaagaattta gaactgagtt
    10321 accactacaa aatactccat atagtaaaaa aaagtaacaa aaaaaagaaa tattaaataa
    10381 aaaaatagat gaaattgtaa ataaaaaaaa cccttttgca taaggtatat tttaaatgat
    10441 agaatataca tatacaggag ggtttttctt ttataaaata agtttaataa aattgagagg
    10501 tgagcagaat gaattttaat aatagtctag aattaactca gtctcagaag ctaataatga
    10561 ctacacaact taagcaatca ttatcaatat taaatatgag taagttagag ttagaagaag
    10621 agataaaaag agaagcagag aataacccat tagttgaagt cgagaaaagt ggggagataa
    10681 attgggaaga gtatataaaa gacatggata ggtcaagacg tttagataga acagagataa
    10741 gctataatcc tgataatgaa gttaatctag aaaatttagt taaatattcg tcaaatttat
    10801 atgaagattt aaaatttcag ataagcctgt ataaacttac agacaaagaa ttggaagtct
    10861 gtgagtatat aattgatagt ttagatgagg atggatattt aagaactgag gaaaaagtaa
    10921 ttgttgatac cttaaagata gatgaagaac tttttgaaaa atgtttaatt agtgttcagc
    10981 agttggaacc tagtggagta ggggcaagaa gtctttcaga atgtctgatg atacaaatgg
    11041 ctagtttagg aatatataat gaaattttag aggaaatcgt tacaaaagac ttaaatttaa
    11101 ttgcaaataa taaatacaaa gagataagta aaaagtacaa tatgtcatta caaaaatgtg
    11161 ttgaactaat aaatataatt aagacattag accccaaacc aggaagaact tgttcagtag
    11221 agaaaagtgt atacattcaa cctgatgtaa cagtagaaaa aattgatgat gaatttattg
    11281 tatatttaaa tgagaaagat tcttatcaaa taagaatcaa tagttattat aaagacatat
    11341 taaaaaattc acagtctgat gaaagtgcaa aagaatttat aaaagaaaga ctaaattcag
    11401 caacaggact tatgaaaaat atagagagta ggaaaactac agtacttaag attgcagaag
    11461 agatagtaaa agctcaagat gaatttttga gaaaagggac aaaatatata aaacctttga
    11521 aaatgaaaga catagcagaa aaattagaat ttcatgaatc tactataagt agaggtgtaa
    11581 atggtaaata tatgctaaca ccttttggcg tatatgaatt taggtacttt ttcagtagcg
    11641 ccattgagac tgagaataat gaaatggcat ctagtacaag cataaaaaaa attataaagg
    11701 aaacaataaa agacgaaaat aaaaagaaac ctttaagtga tgaccatata tctaagttat
    11761 taaaagaaaa aggaatcaat gttgcaagaa ggacagttgc caaatatcgt gaagaattag
    11821 gaatcccatc ttcaagtaag agaaaagaat actaataagt aaaagacaaa tatcagtatg
    11881 gtaaggggaa aaggctaatt aagagatgaa tgtagattgg gctaaaaatt ttataaagta
    11941 aaataatata atatgaaaaa gtattaaaat gctataaggt attaaaaaac tggggaacat
    12001 taaaacatta agatagcaaa gagcataaaa aactaaagaa gattaaaaca ttaaagaaca
    12061 ttagagcttt gaggaatatt agaactttaa agaatattaa aatttataaa atataaaaat
    12121 actaaaaaga attgaaaaac tggggaacat taaaatattg taaaatgcta aaaaactaga
    12181 aaacataatt aaataagtgc ttataaagaa tgattaaatt aaataagtgt ttgtaaagaa
    12241 taattagata ttagaaaaag aagaatacct agataaataa actattatta aatttaataa
    12301 tcacaaaagg aaaacacgta gggtataaat aaaaatctta actagataaa agtaaactta
    12361 gttgtagata aaaataacta g
//
LOCUS       DAYASV010000110        12312 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYASV010000110 DAYASV010000000
VERSION     DAYASV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.111"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            172..867
                     /gene="cas6"
                     /locus_tag="ACGI68_002889"
     CDS             172..867
                     /gene="cas6"
                     /locus_tag="ACGI68_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HFH0139316.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQHSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            879..2618
                     /locus_tag="ACGI68_002890"
     CDS             879..2618
                     /locus_tag="ACGI68_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HFH0139317.1"
                     /translation="MLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKNFSNIDIL
                     EVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKKENLRPDK
                     NNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLNKIRLWIK
                     TNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIIDGKNYGLP
                     NDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLTNKAANGEEVIYISSE
                     KGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIPIEYERHN
                     LKPNLEIGYINNLIKLEKTIDTVFFNKFMCNSYFKEIKNINLSDLVLRDILIRHREGL
                     YTWFYKGRDSIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFHGGSNMAD
                     KIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSLINPILNA
                     QNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLLIGGYLYY
                     NLIYTKSEENENE"
     gene            2611..3600
                     /locus_tag="ACGI68_002891"
     CDS             2611..3600
                     /locus_tag="ACGI68_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HFH0139318.1"
                     /translation="MNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALKFP
                     MKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEDLKLEKNQQKVL
                     ENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPKAK
                     DKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKNDY
                     KKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDLIC
                     INIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3602..4342
                     /gene="cas5b"
                     /locus_tag="ACGI68_002892"
     CDS             3602..4342
                     /gene="cas5b"
                     /locus_tag="ACGI68_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5b"
                     /protein_id="HFH0139319.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYKEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4366..6924
                     /locus_tag="ACGI68_002893"
     CDS             4366..6924
                     /locus_tag="ACGI68_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFH0139320.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEAYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDDFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALKFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
     repeat_region   7049..9983
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7114..7143
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            10068..10562
                     /locus_tag="ACGI68_002894"
     CDS             10068..10562
                     /locus_tag="ACGI68_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HFH0139321.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKTKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            10588..11595
                     /gene="cas1b"
                     /locus_tag="ACGI68_002895"
     CDS             10588..11595
                     /gene="cas1b"
                     /locus_tag="ACGI68_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HFH0139322.1"
                     /translation="MLILGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFC
                     LNEVSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRRE
                     EIAKSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEG
                     DIWQTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYAKTVTAIYNTHLNQ
                     SVSFLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLND
                     EGKQIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDG
                     L"
     gene            11806..12096
                     /gene="cas2"
                     /locus_tag="ACGI68_002896"
     CDS             11806..12096
                     /gene="cas2"
                     /locus_tag="ACGI68_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFH0139323.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
BASE COUNT         5294 a         1075 c         1876 g         4067 t
ORIGIN      
        1 atttttttga aactttgtga ttttatttgt cgaataaaaa ataaaaaatt ttaaaggaaa
       61 aagttaaaaa ataaagaatt ttataactat aaattatatt ttagagttta ttgttattaa
      121 atagatgaaa aaatcctaat atggattatc agaaaatgag gaggttgtta attgataaaa
      181 tgtcttgaaa tgtctgtaaa agtgtatctt cttgaaaata tttctttaga taatacatca
      241 aaagaaatac gtaaattaat agatgataca ttaattatag aagaaaaata taaggaattt
      301 cataataaag ctggatttaa gaattattct tttaatttat tatacccatt agaaaaagat
      361 aaggtttata agaaggattg tatatatact tttattatta gaactgtaga tagtgaatta
      421 ggaatgtatt ttatgaataa tttaaaaaat cagcattctg ataaaataaa ggttattaca
      481 atgagtaaaa aagtgatccc taaaaaacat atagaaagta tttactctgt cactccaata
      541 atagctaaat ttgaggatgg gtattggaga agtaataaaa cagtagaaga tttagaaaaa
      601 agaatcagag aaaatttaat aaaaaaatat aatgaattta ctaacagtaa aatagatgaa
      661 gattttgaat tatttcaagt tattaaattt ataaatagga aacctatttc tactaaatat
      721 aagaatatat ctttacttgg tgataaaata agttttaaag tatcagaaaa tgatatggct
      781 caggaaattg caaattttgc acttggagtt ggagtcggag aaattaattc tcgtggattt
      841 ggttttgtaa actatagatg gttataggag gtgaaaactt gttaaaggat tgtttagaga
      901 tttttgaaaa agaatacaaa tctttaggaa atagattgat aactgatagc tatgtcctat
      961 cctatggaac atatatattg gtgaaaaatt tttcgaatat agatatatta gaagtttcta
     1021 aaaataatgt ggatagtaca gaagatatat accataaatt ttgtgaactg gattatttat
     1081 caaaactggt gtctatagat aaatcagttg attccaaaaa aataatacat agtaataatt
     1141 atctttcatt ttttgtaaaa aaggaaaact taagacctga taaaaataat aatgtaaaac
     1201 ttacagaaga aattattgat aattattatg atgctttgtt aaacccaaga attaaatata
     1261 aatctgataa aagaaaaaaa gaaatctatg attcaataga aaataaatat ggtaaagtgg
     1321 atgaaaataa attaaataaa ataagactat ggataaaaac aaatatattt gatttagttg
     1381 aatctgttaa aaaagataaa aattatttaa aaatattttt tgatgaaaaa atagagttat
     1441 acaatgatga aaaccaaaaa tatttatatc caaatatata taataaaaat gatttcaatg
     1501 taattattga tggtaaaaac tatgggttac caaacgataa tatgcaatta aatgaaaaaa
     1561 agccattttt gaaattaaaa acaagaatta gaaattcaac tccttatttg attagtgatg
     1621 atgaagtttt gttacagaaa gctttttttg attatctaac gaataaagca gcgaatggag
     1681 aagaagtaat atatataagt agcgaaaagg gaattcatag aaaaactgat ataccaaatg
     1741 aatttagtgg ttatatactg agaataaaaa agggtaaaga attagaaata catgattttg
     1801 atactgtggt aaatttaaac actaaaataa aaagaataaa tgtagaatgt ataataccaa
     1861 tagaatatga aagacataat ttaaaaccaa atttagagat aggatatatt aataatctta
     1921 taaaattgga aaaaacgatt gatactgtat tctttaataa atttatgtgc aatagttatt
     1981 ttaaagaaat taaaaatata aacttaagtg atttagttct aagggatata cttattagac
     2041 atagagaagg tttatatacc tggttttata aagggagaga tagtatagta aagcaaactt
     2101 tttcaaacta ttctatgggt ataattgaaa attctataaa aaacaataac tacataagag
     2161 caaaggagca atataattta agaatagcta taataaatta ttttcatgga ggaagtaata
     2221 tggcagataa aataaaagtt attagtgaat ctttatttga aaaaataaat tctataaaaa
     2281 cgaaatgtat acaaagtgat gaagaatatt attttgcagt tgggcaatta gcatcgtatt
     2341 ttatatctaa aaataagtca aaaaataaaa atcattctct gattaatcct atactaaatg
     2401 cacaaaatag aaaaaaaatc atgttagaga tagaaaaatt atttaaaaaa tataattatg
     2461 atataagtta tgacaacaat aggcgattta agaatctata ttctatgatt gtaggatatg
     2521 aggtagaaga aaaaaaagtt aatagagatt tattaattgg tggatatcta tattacaatt
     2581 taatatacac aaaaagtgag gagaatgaaa atgaatagaa gagtatatgg agtattaggt
     2641 ataaaatcta taatgtcaaa ctggaatgct gatttttctg gatatccaaa gacgacatca
     2701 acaggagaag tttttgggag tgataaagca cttaagtttc ccatgaaaaa aatgtgggaa
     2761 aatgaaggtg aaaaagtttt atatgttaaa tcattaaaaa tagatgaatc taagaaagga
     2821 gaaataaaaa ttagaccaaa atcattaaaa gagagatatg aagaattgtt tgatatagag
     2881 gacctaaaac tagaaaagaa tcaacaaaaa gtactggaaa atctatttaa agctattgat
     2941 gttaaaaatt ttggtgcaac atttgcagaa gaaggaaata atataggaat tatgggcgca
     3001 gtacaaattg gacaaggatt taataagtat gaagatatta atgttgaaga acaacaaata
     3061 ttatccccat ttagggatcc aaaagcaaaa gataagaaaa agtcaacaaa tgaagaagaa
     3121 aaagaagatg caaattcaac tacattagga aaaaaaatag taagtgatga ggctcattac
     3181 ttttatccat tttcaataaa tccaaaagct tatgaagttt ttgttaatat gggtgtgact
     3241 gatggatata ctaaaaatga ttataaaaaa tttaaagaag ctgctttagt aagtgcaact
     3301 gcattcaata caaattcaaa gcttggttgc gaaaatgaat ttgcattatt tgttgaaact
     3361 gatgaagagt tatacctacc agatttggca caatatataa cttttgaaaa gagagaaaaa
     3421 tcaaaagatt taatttgtat aaatataatt catcttatag cagaattaaa agatgatata
     3481 aagagcgttg agatatatta taacccatat aaaatagaaa taaagccttt taaagatgaa
     3541 atattaccta agatagataa agttaaattt atgaacatat ttacaagaaa agaggtataa
     3601 tatggaggtt ttgaaattta acttaagtgg aaaaactgct ttttttaaaa aaccagatgt
     3661 aaatagcata atttatttta cgtatggaaa catacataaa attgcactta tgggtatttt
     3721 aggagcttgt ttaggacttg gtggatataa tcaacagagt aaggaagatg agtatccaga
     3781 attctactct ttacttaagg atataaaaat gtcaatagtt ccaaagcatg aatttggata
     3841 tataaataaa aaagtccata tatttaataa ttctgtagga tatgcatcaa aagaagaagg
     3901 tggaaattta ataataaaag aacagtggct tgaaaatgtt agttgggata tatatataca
     3961 attagaagat tcaactacaa gtagagagct taaaagaaga tttttatcta ataaatttgt
     4021 atatatacct tacttaggca aaaacgatca tatagctaat ataagtgaaa tagagataat
     4081 tactgatttt tacaaaacag aagatattaa cagagtggac agtttcttta taagagagga
     4141 ttttgagttt gaaaaatctt attttgatga taatgatgat tataaagagc catttaaata
     4201 tcaggaaaaa cttccttata gactgcaccg aatgactaat caatatattt tggaaactat
     4261 catatttact aatatgtcag ttaaattgat taataaaaaa gaggtatatc aagtaaaggg
     4321 tttaaattta atctttattt aaatttatag gtttaggaga attatatgta ctttaaaaaa
     4381 gttgagttgt ttgatataag taataatata aataattgca aaaatattta tgctcatata
     4441 aataaagaca ggaagccaga aactttaaaa gaacattcag acttatcata ctcatatctt
     4501 atgaaattat gtgtaaaaaa agatttaagc tcttgcctag agagaataga gaataaattt
     4561 tttaaaaaag attctttttt aagtaagaat ttttatagag agattatctt gaatatggtt
     4621 tatatgcatg atataggtaa gattaattct tcatttcagt ttgtaaaaat gcaaaataaa
     4681 aattttaaaa aaatttcact tagatatact aaacattctt ttatctcttc aatgctatat
     4741 tttgattact attttgaaaa gcttaaaaaa tatgattttg aagaaaaaaa aatattgagt
     4801 ttatttttga tgataaatac atatacaata tcaaaacatc atggggttct gaagtcattt
     4861 aaagaatttt tatttttatt taaagatgag tttataaaca ttaaagaaaa ttttaaagat
     4921 ttatttaaaa acaattataa tttagagctt aaatcagttc ccaaaactat agaaaaaatg
     4981 gtaagaggtg tagaagcgta tttagatgat agccatataa aggataactt agctatagac
     5041 ttatatatat attcaaaatt gatgttttca attgttacat cttgtgattt ttatgctaca
     5101 tcagaatata atagtggtaa ttctatagat gattttggga caattgataa ttataataag
     5161 tattatgatg tattaaaaaa atctgaaata tataagggga tgaaaaaata tagggaatac
     5221 ttaaataaca atagtgaatg cccatataaa gagaatgata taaacagatt gagaacagaa
     5281 atgagtatag aagctgaaga tgcaattaca cataatacga gaaaaagaat attttattta
     5341 gaagcaccaa ctggaagtgg aaaaactgtt acatctataa atctagcact gaaatctata
     5401 gagttaaatc aatctttaaa aaagatattt tatatatttc catttaacac attagtagag
     5461 caaacaaaag atgtatttat aaatgaaata tttaattctg ttaaagacat acaaaaagat
     5521 gttgtagtta taaattcaat aacaccaata caaacagaag ataaagaaga agataataaa
     5581 aatgttgaat attgtgttac taaacaagaa attgattatg ataaatcatt gttaaataga
     5641 caatttttac actatcctat agtacttaca actaatatag gtttttttaa ctatttgttt
     5701 ggtgtatcaa gagaagaatg ttttccattg atacatatga taaatagtgt aattatatta
     5761 gatgaaatac aaaattataa aaatgagata tggaaagaaa ttattttgtt tttagaaaaa
     5821 tatgcagata tattaaatat aaaatttatt ataatgtctg caactttacc tagattaaat
     5881 aaattaggat gtaatgatga taattttgca tatctagtaa aaaacagaga aaaatttttt
     5941 aaaaatcacc tttttaaaga tagagtaaac ataagttttg agatgttaaa ttcagaaatt
     6001 aatgatattg aagaaatatc agaaaaggtt atagaagaag cagaaatata taataagata
     6061 ctgatagagt ttataaagaa atcatcagca ctaaaatttt ataaaaatat taaagaaaaa
     6121 ttgaagtata agttagaaga tgttttttta ttaacaggtg atgataataa attagaaaga
     6181 aaaaaaataa taaataaaat taaagatatg aactctgtaa ttttggttgc tacacaagtt
     6241 gtagaggctg gagtagatat agatatggat ttgggattta aagatatatc tacaattgat
     6301 tctgatgaac aatttttggg aagaataaat agatcttgta aaaaattaaa ttcaaaagta
     6361 tactttttta atttagatga tgcaagtaag atatataaga aagatgttag aaaagagaga
     6421 catttaactc ttaatgagga aagtaataga aaaattattt tagataaaga ctttgaaaag
     6481 tattatgata aaataatagc cagaatagaa gaaaataagc ataaacataa tgacaagaat
     6541 atagaggttt ttataaaaga tattgtggga aatttgaatt ttacagaggt aaaaaaatgg
     6601 atggctttaa taccagattt aaaagagtat acaatctttt taaatactat agttaaagat
     6661 gaatctggaa atatgattgt aggaagtgat gtatggtatg aatatatatc actactaaaa
     6721 aatgataatg ttgagtattc tgaaaagagg gtgaaaatgt ctgaaattat ggaaaaacta
     6781 gattatttta cgtataaagt tcagaaattt gataattcat ttaatgattt agttggagat
     6841 attttttata ttgatgatgg aagtaagtat tttacagagg gaaagtttga tagaagtaaa
     6901 tttaatcaaa atgaatttct ataatgtatt tagattatat agagaatatt tatcccaagt
     6961 taattaatta gaattagttt aaaactattg aaatatagat attttatagc ttatatattt
     7021 tttctaggat tagctgggat aaaaaatctg tatattagtg ttttcagtgt atttaagggg
     7081 attatatttt taagaagtac tgattttaaa gtggttaaac agtaacatga gatgtattta
     7141 aatttttact acataattaa atcgtagtca ataccgtaca ggttaaacag taacatgaga
     7201 tgtatttaaa tctctgaata gccaatcaac tttgctgcat tactcttgtt aaacagtaac
     7261 atgagatgta tttaaatgat aatagttata tatatttttt acaatatatt attgttaaac
     7321 agtaacatga gatgtattta aatttacttc tcctgttttg aaatctatat attcatcatg
     7381 ttaaacagta acatgagatg tatttaaata taatattgat acaaatattt tgggtaaatg
     7441 gacttgttaa acagtaacat gagatgtatt taaattcgat atggtaacaa atttataaaa
     7501 gatatagaat gggttaaaca gtaacatgag atgtatttaa ataaagaaaa acgaaattgg
     7561 tgattttgaa gcaatgtgtt aaacagtaac atgagatgta tttaaattag taactacaat
     7621 gcttttcctt ttgaagatga tatgttaaac agtaacatga gatgtattta aattaataat
     7681 gcacctattt taacagtacc tggtctaaag ttaaacagta acatgagatg tatttaaatt
     7741 caagtctagg agacaacata aatagtacag atgggttaaa cagtaacatg agatgtattt
     7801 aaattagtaa aaccagatgg aactgttgta ataccaccaa agttaaacag taacatgaga
     7861 tgtatttaaa tttaaaaaca tcttgaggaa tatctttaac agtaattgtt aaacagtaac
     7921 atgagatgta tttaaatgca ttttcaggtg catgataaat aggtaaatta gatgttaaac
     7981 agtaacatga gatgtattta aattttttgt aattagccag attatcaact cccaaaatag
     8041 ttaaacagta acatgagatg tatttaaatt aaatatcgtc cttcgtagtc aaagcaacac
     8101 cagaaggtta aacagtaaca tgagatgtat ttaaatcgta aacaaaattt atcaagagaa
     8161 gaatttctaa ggttaaacag taacatgaga tgtatttaaa tttatatttg ataagagtat
     8221 caaatcgact taaaatagtt aaacagtaac atgagatgta tttaaattca atcttaatac
     8281 acagacattt ttaaaaacaa aacgttaaac agtaacatga gatgtattta aataaataga
     8341 caaatattga atagctctgt cttcatcaag ttaaacagta acatgagatg tatttaaata
     8401 tatttacaaa atctctaaca tattttttaa cttctgttaa acagtaacat gagatgtatt
     8461 taaattcttt taaagagtcc tcgggagcct cgaaaacttc agttaaacag taacatgaga
     8521 tgtatttaaa ttactcaaat aaatttctaa tttgtttatc tactaacgtt aaacagtaac
     8581 atgagatgta tttaaattag aatttacaac aatataagcc tctttaacca taagttaaac
     8641 agtaacatga gatgtattta aattgtatat ttgatgttgt tatttctcct gtgtataatg
     8701 ttaaacagta acatgagatg tatttaaatg aagttccacc cccaccaccc ccactacttt
     8761 gagaagttaa acagtaacat gagatgtatt taaattaaca atagagtaaa tataataaac
     8821 aagcatacaa agttaaacag taacatgaga tgtatttaaa tccccttttg catggtacta
     8881 gacacgcata tatggtggtt aaacagtaac atgagatgta tttaaatacg cctatatgcg
     8941 gagtaacaga tttttgcctg gatggttaaa cagtaacatg agatgtattt aaatactgtt
     9001 ttgggataga ctggaattaa atggtctgtg ttaaacagta acatgagatg tatttaaatt
     9061 tttggaatga agcatcaaca gataataaag aaaaagttaa acagtaacat gagatgtatt
     9121 taaatcaagg aaacaaaaga gaagtgaaaa gaaatataat gttaaacagt aacatgagat
     9181 gtatttaaat aatcttttaa atggaaatta agtaataaaa gagagggtta aacagtaaca
     9241 tgagatgtat ttaaattcaa ttaaataagc attaatctta gtaaaatcaa acgttaaaca
     9301 gtaacatgag atgtatttaa attttcttgt gttaattgaa cccccatttt aagctccagt
     9361 taaacagtaa catgagatgt atttaaatca gaatacagct tctatatctg gatacatttg
     9421 tcttggttaa acagtaacat gagatgtatt taaatgtgaa aagtaataca ggtatagagt
     9481 ctaaacgctc ggttaaacag taacatgaga tgtatttaaa tcaatgtatc ctctttttta
     9541 gttttggagc ctcttttgtt aaacagtaac atgagatgta tttaaattag ttagcacatt
     9601 ttatttcaag ccttaaaaca ttcgttaaac agtaacatga gatgtattta aattaatttg
     9661 cacacttaac ctctaatctt aaagtattag ttaaacagta acatgagatg tatttaaata
     9721 acacaatatt atctttaata gaattaaaca aatgagttaa acagtaacat gagatgtatt
     9781 taaatttttg ttggtcttgt gcttctttta aaagctgatt agttaaacag taacatgaga
     9841 tgtatttaaa tttaaaaagt tctgttatta aaacatccaa accaatcgtt aaacagtaac
     9901 atgagatgta tttaaattct gtaccatcta gccaaaccgt ttttgaagtt aaagttaaac
     9961 agtaacataa gatgtatcta aattactcta tcataaaatt ttaatatatt aaacaaacta
    10021 atatgtgata tacttatgta taaataacaa aataaatagg tgatgttatg aaaattacag
    10081 gaactttgat aaattactat ttccattgta aaagacaatg ctggttgctt ggtaatagaa
    10141 taaatttgga agaaaatagt gaagatgtaa aaataggtag acttcttcat gaactaaaag
    10201 aagaaaaaac aaaatataaa gaaattgcaa ttgaaaatat aaaaatagac aaattaacaa
    10261 gagaatattt agtagaggtt aaaaagtctg atgctgatat agaagctgtt aagtggcaag
    10321 ttcttttata tttaaaaaag ttaaaagaaa agggaattgt aagaaaaggg aaaattgaat
    10381 ttatagaaaa aaataaaact aataaaaaaa tagtctttgt agatttgagt gaagtatctg
    10441 aagaacagct tatatctata gaaagagaaa ttgttaattt aataaatagt gataaagcac
    10501 catgtcctga tttaaaaaaa ggatgtaaaa aatgtgccta ttatgaatat tgttatatat
    10561 aaagaggtat aagaaacata taaggaggtg ttaatattgg gaaagacaaa atatattacc
    10621 tcaatgggtg aactcacaag aaaagataat tctatatgtt ttagaaaaaa tggaaggaat
    10681 gtatatatac cagtagaaaa tactagtgaa atattctgtc taaatgaggt atctttaaat
    10741 tcaaaattac tggatttttt atcaagagca aatattgttg ttcatttttt taattactat
    10801 ggtggatata gtggaacttt ttatcctaaa gaatatttaa taagtggaaa agtaacagtg
    10861 gcacaggtaa aaacctttga agatagaaga gaagaaatag caaaatccat agtcaatggt
    10921 ataaggatta atgcaataaa tacattatcg cattactata agcatggaaa ttcagaaata
    10981 caaccattat tatcacatct aaaaaaagat tctataaaca agttagaaaa atcaaataat
    11041 ataaaagaaa tattatcagt cgaaggagat atttggcaaa cattttatac ttcctttaag
    11101 tatattttaa gagaggaatt tatttttaat aaaagagtta agagaccacc agataatccc
    11161 ttaaacgcaa tgatatcatt tggtaacagt attttatatg ctaaaactgt aactgctata
    11221 tataatacac atctaaatca aagtgtaagt tttttacatg aaccaagtga aagtagattt
    11281 tctttaagtc tagatttaag tgaaaatttt aaacatgtta taacttttaa aactatattt
    11341 gatttagtta ataatagaaa aataaatatt gaaaaacatt ttgataaaaa tttgaactac
    11401 tgtgttctaa atgatgaagg aaagcaaatt tttataaatt ctttagaaga aagattagaa
    11461 tcagtcaaag aacatccaaa attaaaacga aaagtaagtt taaaaacctg tataaaatta
    11521 gatgcttaca aaatgttaaa gtgtataatg gaagaaaaaa gttttattcc atataacata
    11581 aaagacggat tataatttaa acataaagta agtattttaa tatagaactt tgatatatat
    11641 aagaatgata attatgctat tataagtata cataatatat tattgaaata atataagttt
    11701 ttagaagtat tttaagatga gaaaggagaa aatactgaaa aataattatt ctattataat
    11761 ttgccaaggt tttgtatatt tttcagttta aatattatat tacatatgaa aaagaaaaac
    11821 aatttaaatt ataattatgt atttgtagtc tatgatgtag gagaaaaaag agtaagtaaa
    11881 gtttttaaag tgtgtaaaaa atatctctcg cattttcaaa aatcagtttt tagaggtgaa
    11941 ataactccat ctaagattat gagcttaaaa cgtgatttag aaaatattat agataaagat
    12001 aatgattttg tatgtataat aaagttgatg aattctaata catttgaaga agaaatatta
    12061 gggaataata gtaattgtga aaatatgata ttataaatct aattactata tttgtaaaaa
    12121 ataatagctt attaaagagt agtaaaattg ggatataatt tatgatagag actgcaagta
    12181 gatttttgtg aaaaggattt acttgcagta tttttgttat gtataactgt taacattagt
    12241 gtacaaaaca ttatcaatag catttttcta taatgaagaa atactattat aatgggaaaa
    12301 ttttattaat ac
//
LOCUS       DAYASV010000111        12199 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYASV010000111 DAYASV010000000
VERSION     DAYASV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.112"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            291..1682
                     /locus_tag="ACGI68_002897"
     CDS             291..1682
                     /locus_tag="ACGI68_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0139324.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            1935..3728
                     /locus_tag="ACGI68_002898"
     CDS             1935..3728
                     /locus_tag="ACGI68_002898"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFH0139325.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIAPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(3809..5191)
                     /locus_tag="ACGI68_002899"
     CDS             complement(3809..5191)
                     /locus_tag="ACGI68_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFH0139326.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPINGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            5449..5796
                     /locus_tag="ACGI68_002900"
     CDS             5449..5796
                     /locus_tag="ACGI68_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0139327.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            5814..6848
                     /locus_tag="ACGI68_002901"
     CDS             5814..6848
                     /locus_tag="ACGI68_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HFH0139328.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(6897..7946)
                     /locus_tag="ACGI68_002902"
     CDS             complement(6897..7946)
                     /locus_tag="ACGI68_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFH0139329.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            8221..9756
                     /locus_tag="ACGI68_002903"
     CDS             8221..9756
                     /locus_tag="ACGI68_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139330.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(9941..10627)
                     /locus_tag="ACGI68_002904"
     CDS             complement(9941..10627)
                     /locus_tag="ACGI68_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HFH0139331.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            10752..12134
                     /locus_tag="ACGI68_002905"
     CDS             10752..12134
                     /locus_tag="ACGI68_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0139332.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
BASE COUNT         4663 a         1480 c         2024 g         4032 t
ORIGIN      
        1 atatttttaa tttgaatttt ttcaataaaa atttacaaaa atgaaaaaat aaacaaaaaa
       61 gaattgaaga tttaagttgt tatttaaata tatatatatt aagattttct gctgaagata
      121 ctaaaaaaat aatatttttc ataattttaa aaataataat aaaaaaatga aaatacaggc
      181 tataatctat tgagtaaaca tagaaaagat gatattataa gtatgtgcta aaatggttaa
      241 agcaatatta atattaaatt taactataaa acaaaagggg gcgttttttt atgtcaagta
      301 cagaaagcgt atctaaaaaa agtggtcatc cgaagagttt ttggcttatt tgcatgacaa
      361 ttttgtggga aagatttagt tattatggtt taacagcttt attggtatta ttttttacag
      421 caagtgtagc tcaaggtggt ttaggtcttt ctactcaaga agcaacatct atgtttggct
      481 tatttacagg tcttatatat cttacaccat tagtcggtgg ctggttagca gataaagttt
      541 taggtcaaca aaaatgtatt tctataggtg cacttttaat agcaatagga gatttcgtac
      601 tatttgcttc atctacatct attgcaatgg tttgggtagg tctatcaata attattttag
      661 gaaatggttt ttttaaatca agttgctcaa atatagttgg ggatatatat cctaaaactg
      721 atgtgtcaag aaaagatgca gcatacagtt tattctatat ggcagtaaat gtaggagcat
      781 ttatagctcc aataatatgt ggattgcttt ctgataatgt atttgcagta agaggagcaa
      841 gtggagatat agtatcttat ggatataaga tggcattttt aatttgtggt ataggcatga
      901 tgttaggtat gattatattt acacttcttg caccaaaatt attaaatgaa gtcggaaaat
      961 atcctgtgac taaaggtaat aaaggtgaaa aaattgagta tggaccactt actaagttgg
     1021 agaaaaatag gataatagca atggttattt tatttttatt tgtggtctta ttctggacag
     1081 catactatca aacacaaagt tcttttatga tttatgtaag ggataatgta aatagaactc
     1141 tatttggatt tgaaatgcca gtagcatggc ttacatcact aaatgctata ttatgtgtgg
     1201 ctttatgccc agtattagct accctatggg ttaaattatc tcataccaaa agaggtgatt
     1261 tctctattcc agttaaaatg ggattaggaa tgataataat gggtgttggg tttatagtta
     1321 tggtatttgc tacattagct aatggtccag atggaatggt aaaggctagt atattgttta
     1381 tagtattgac ttatatattt acaacagtag gtgaattatt cttatctcct ataggacttg
     1441 caatgttcaa taaattagct ccagctaagt atgctacgtt gtctatggga gcctggtatc
     1501 taacaagttt ttttgcaagt ttattatcag gaaagattgc tggttttaca gccacacttg
     1561 gttttttaca gatatttgga ggaatagcaa cagttttaat aatatttggt ttaatattat
     1621 tgggaataag aaatgtatta acaagattaa tggctatgga tttactaaca aaagaaaaat
     1681 aattcatgac gaattattgt aaataaatga attactttaa ataaaggaag ttacatttaa
     1741 atataaaatt aaatgtaatt tcctctttga ttatgaaaaa aagctttaca caaatagata
     1801 ttataattaa aattataatt gtgataatta gagtgtataa gatttatata aattatacaa
     1861 aaaatattta tgtaataaac tttacatact tatatattta gttattcaaa ctattatttc
     1921 gaggaggtct tgttgtgaat ataaaagata gattaagtgg attaagaaaa tttatggaag
     1981 aaaaaaatat agatgcctat atgattccat cttctgataa tcaccaaagt gaatatgtag
     2041 gagattactt caaatcaaga gaatttatat cagggtttaa tggttcagca gggacggtta
     2101 ttgtgactaa agacgaagct gggttatgga cagatggaag atattttata caggcagaat
     2161 ctcaattaga aggaagtaca ataaaattat tcaaaatggg acaagaaggt tgcccaacta
     2221 cagatgaata tttatataaa aatattcctg aaggaggaac tttaggattt gatggtagag
     2281 ttatatctgc tagagaagga gctacattag ctgaaaaatt atctaaaaaa ggtataaaga
     2341 tagaatatca gtatgattta atagatagta tatggccaga tagacctgct ttatcagata
     2401 gtaaagcatt tttattagat gttaaatatt gtggagaaag ttttagctca aaattagcta
     2461 gattaagaga aaaaatgagt gaaaaaggta cttctactca tgtaataact actcttgatg
     2521 atatagcatg gttattcaat ataagaggtg gagatgttaa atataatcca gttgtacttt
     2581 cttatgcagt aataacttta aaagaagttt atctatttgt tgatgaaagt aagttaaatg
     2641 aagaaatact aaatgaacta gctaaggaaa atgttcaaat aaagccatat aatgatgtgt
     2701 atgaatttgt aaaaaatata gacaaaactg aaaaagtttt attagatgga actaagctta
     2761 gttatacaat ttacaataat ataccttgtg aagttgagaa agttgatgag ttcaatccag
     2821 taatgttttt taaagctcaa aaaaatgagg tagaattaga aaatataaga aattcacatg
     2881 taaaagatgg tgtagctttc actaaattta tgtactggtt aaagaaaaat gtaggtaaga
     2941 tggaaatcac tgaaataagt gctactcaaa aattagagga cttaagaaga gagcaagaag
     3001 gtttctttga gccaagtttc aatacaattg ctgcatataa agagcatgct gcaatgatgc
     3061 actatagtgc aactccagag tcaaattata aattagaggc agaaggatta tttttagtag
     3121 attcaggtgg acaatattat gatggaacta ctgatataac taggactaca gttttaggac
     3181 ctataagtga tgaattaaaa ctacacttta cttctgtagc aagaggaatg ataaatcttt
     3241 ctaaggctaa gttcttgcat ggttgtagag gatataactt agacatacta tctagaagtt
     3301 gcatgtggaa tatgggaatt gattatcaat gtggaactgg acatggtata ggatttgtgc
     3361 taaatgttca cgaagctcca aatggattta gatggagagt agtacctgaa agatttgata
     3421 gtgcagttct tgaagaagga atggtaacta ctaatgaacc aggtatatat atagaaggtt
     3481 ctcatggtat aagaacagag aatgaaattg tagttagaaa agctgaaaag aatttctatg
     3541 gtcaatttat ggaatttgaa gttgttacgt tagctcctat tgacttagat ggtatagcac
     3601 cagaattaat gaataaggat gaaaaagatt atttaaattg gtatcataag ttagtttatg
     3661 ataaaatatc acctttctta actgatgaag aaagagaatg gttaaaagtt tacactagag
     3721 ctatatagtt tgtactagat acaataaaaa tcgaggtatt ataatgcctc gattttttat
     3781 tgtatatttt attaacttta aaaaattttt aatttgataa ttctactaat tttttattca
     3841 ttataagtag tataattcca aatatcaata aaaatgatgg tatcactatg aagacttgta
     3901 acataccaag ttggtctata atagcttgcg tatagccaga taatttattt gctgcaaatt
     3961 tgcctaaaaa ccaaacaccc attaggaatg aaatatactt ttcaggcgaa agtttattta
     4021 ccataccata cccgactgga gaaaagcaaa tttctcctat actttggaat gttaaaaatc
     4081 caactaccca aaatacactt gctttgtgaa ttgaattagg gtctgttcct gtttgtataa
     4141 ctgctactat cataaacaaa aatgctatgg ctaatagtat aaaacctaag ctcatttttt
     4201 ttggcacact taaatcgcct tttttagttt gagataattt tacccatact aaagcagaga
     4261 taggtccaag aataacacaa aggagaccat tgtatgaatc tatccatgaa gtaggaattt
     4321 gaaaattgcc aacagtaaga tttatgtgtt cctttgtata gagtgcaata gacatagagg
     4381 cttggttata tgctatccaa aaaaatactg tgaaaaaagc taacaccaat attactatta
     4441 ttcttttttt ctcaatcaaa gttaaaggtc ttgaagcgag cattttctta tattctagtg
     4501 attttcttat agactctctc ccgttaatag ggtcattatt caaaggttta taaaactttc
     4561 tgtttccaaa tataaagaag aataaaccta ttagaaccgt aattgatgaa aataaaaata
     4621 catatctata tccatacata cttatagata atccgctttt catagcaaac cattcattag
     4681 caactaaacc gcatattaaa gggccaaata aagtacctag attagtagcc atatacgtaa
     4741 tagagaaagc tccatctttt cttgggtcat cattggtata taagtttcca accatagtat
     4801 gtaagtttcc tttataaaaa cctattccta atccagataa aaatatcatg atgtttatat
     4861 ccatcttact atttgctatg tatcctagaa aataaccaat tgaaaccatt agcattccaa
     4921 gtataataca gtttttggga cctaaaaatc tatcactgac ccatccacca ataactggag
     4981 agaaataagt ccatgctaaa aaagatgcaa gcattgatgc ggattctgct ggtgttaacc
     5041 ctaaaccacc tttggagact tgttcagcta aaaacaataa taataccaat ttcataccat
     5101 agtatgcaag tcctacaaag gtaaatattg aacagcagaa atataaatct ctaggttgtt
     5161 taagtttttc ttgcgttttt aaaactgaca tatgtcgaga acctccagat aaatagttta
     5221 acaaaatttt accatactat atgagtatat gtttttttca tatagacgtc aacttgtaag
     5281 ataatttaat taagtcgaaa tatatgtaaa aatagtatca tttttgtgta ttcagtaata
     5341 tttttttatg ataatatatg ttattattat attaaaatac aaacatattt atggaaagga
     5401 tatgccaatt tagtagttag attgattgtt tggcaggtgg gatgagctat gataagaaag
     5461 aaatctgaat tacaagtaga agtaaaagaa aatttaaatg gtggtatagg aaaggttaag
     5521 cttgaaaaca taatacaaaa tgaagagctt aaaggaaaag gaagactatt taaaagagta
     5581 gctttaccag taggaagttc aattggtgtg catgaccata caacagattt tgaagtatat
     5641 tatatactaa agggaaaagg taaagtattt gataatgggg aatttgtaga agtaaatgag
     5701 ggtgatgtag tgtatacagc tgatggcgaa aaacattcta tagaaaatat aggagaagaa
     5761 gatcttgagt ttgtagcact agttttatat atgtaaaata tggaggagga gtcatgacag
     5821 gcaagataga agaaattaag gacaagataa atagattttg caactccaat ttaaatcaag
     5881 aatacaaaga tataagcttt aaaattttgc aaaacctttt agataataat aaagagataa
     5941 ttcattcttc tagagctgat atatggtctt cagctatact aaatatagtt ttagaacaaa
     6001 atcttttata caataagaag catccactac atataacaaa aaaagagttt tcaaaacaaa
     6061 taggtgtttc tttaaatact ataaataata aatcgtcttt aataaaagat gtttgtagta
     6121 ttgacttttt aaatcaagat actatagcta tttctaatgt agaattttgg gtaaaagaga
     6181 ctaattccaa atcaaagtct gtagatttag atttagaaaa gaagaaaata ctatataaaa
     6241 gatatattaa gctatcacaa gatgcgaata gtgatattga atcaattaga tatatggagg
     6301 aagctgtaaa tataggtaaa agtatgatta ctaaagaaga taaagaactt ggattttgga
     6361 aaggtttatc taccagagcc tatatgatag cacttgaatc ccttgctaga aagttagaaa
     6421 gtattaataa tctaaaggag tccaaaaaag tattagaata tttaatagaa attaatccta
     6481 gtgatgagca gggaataaga tacaaattat taaatgtatt aattagatta aatgatagaa
     6541 cttcaataaa taaattattt gaactttata aagaagaaaa atctgctaca tggatgtaca
     6601 gtaaagctct ttattacttt aaaaataaaa gtatgttttt ggcaaatgat tctattaagg
     6661 ttgctagaag taaaaataag tacgttggat tatatttgtt agattggcga aatgcttttg
     6721 gtagagaatt tgttacagag gaagaaaagg cagaagctgt ctattactat gatgaaaaca
     6781 tagtgatttg gaatgaagtt aaaggttcta tggattggct attaaaaaaa atgttggaat
     6841 tttcataaaa aaaactactc cttactttta tataagtcaa aggagtagtt tttaatttat
     6901 acatctagtt ttaaatcttc aggttttatt agaccttttt ttctcataaa ttctgaaatc
     6961 aacaaagtta caatagcagg aagtataaaa ttaagtaaaa gtataccaac ccacatagat
     7021 atacctcctt ttccagcact ttgcatagca gttactgttc caaattgtcc aaccaatcca
     7081 caagtaccca taccagaacc aataggaata ttctccattt tgaatattgt tgtagataat
     7141 ggtccaagta atattgaagc tacagtaggt ggtatccaaa ttttccaatt ttttattata
     7201 ttaggtactt gcaacataga tgtcccaaga ccttgtgcca caaggccacc aactccattt
     7261 tctctaaagc tcataacagc aaagcctacc atttggcaac aacaacctac agttgctgca
     7321 ccaccagcaa gaccacctaa acttaacatc atacaaatag ctgcactact tataggtaat
     7381 gtaagagcta ttccaacaag tgcagagact aaagctccca ttattatagg ttgcatagtt
     7441 gtagcttgca ttattatatt tccaaatgaa gtcataaaag cagaaacagc tggaccaata
     7501 agttcagcta caagtactcc aattagtatt gttacagcag gtgtaaccaa tatatctatt
     7561 tttgtttctt ttgaaactaa ttttccaacc tctacagcaa aaagtgtagc gacaaatgct
     7621 ccaacaggtc ctcctaattc attaccacaa gctccaacaa gtgcacaaga aaatagtaca
     7681 agtggaggtg cttgaagtcc atacgcaata gccactgcta tagctggacc agtaacctgt
     7741 ttagcaagag ggcttataac ttcagtaaaa agagaaatat tgaaattatc tcctaatgta
     7801 ttgaaaattg ttccaattaa aagggacgaa aaaagaccga gtgccatata acttagtgcg
     7861 tcaataagat acctttgaac agaaatttca atattttttc ttttagcaaa tgatttgaaa
     7921 tttctttttt tataattgat ttccaattta aaatcctcct catataatat aagtataatt
     7981 tctttttaat taattatata acttttcagc tgacaagaca aatgttattt cactaaaatt
     8041 agactattgt aatagtaaga aattaacatt aattttagtt ttaaatttta aatttatggt
     8101 ttataattag tatagggata ttctgaaata taataatttt ccagtttatc ccaatattta
     8161 acttaggtag attgcttaca aaacaaatga ttatatatta ggctaaaagg aagtggatag
     8221 atgatatttg taaaaaataa gaaatacaga aaaattgcca taattatatc tattcctata
     8281 ttgcttgttg cgcagtacta cctttttaaa tttggaatac ttagaccaat ggtaaaggga
     8341 gtagaaattg aaattgtaga tggggaatat gttaaagata tagataaata tgtagtaaaa
     8401 ttaaatgaaa cagttacttt gtctacaggt gaatatatta aggttccttc gtatgctaaa
     8461 aatccaaata tttggtttaa tgtattggat aactcaggga ctttaaaaat agatggaaat
     8521 aaaatgacag cattaaaggt tgggtattct tctgtgggta tcatgaaaaa ttcaagagta
     8581 ttaaaaaagg ctactataaa agtagtagac ccagaggtag aatcactaga tatggagatt
     8641 aatggggatt taaaacatgt tggtgatagt gctactatag aaagtacaat agaagttgat
     8701 tatgataagt ttaaaaaatc atataagcca gtttataaga gtagcaatga aaatatttta
     8761 aaaatcaaag gaaataaagt aagtgcagtt ggagttggta aagctacaat atatgctaag
     8821 tgtggaaata aagaaattga gagaacattt aggattcaag ctagagttgc tgaaataaat
     8881 atggatgata taaaaataga agttgaccag aatgaaaaat tgaaaccaga tataataact
     8941 tctccgcctg gtttagaacc accaaaaata tattatgagt tagtagattc aaaattacct
     9001 gtagaaagag caataagttt aagtagtaat ggaaatgttg tagggcttag agaaggtaaa
     9061 gatagggtga gaataacttg tggagataaa tctaaagtaa taacagtaac agtagtcaaa
     9121 gaaactataa cgaatgacta tatacaaaac ttggttggtt cacataaaat agtaaacaat
     9181 aaagttatta ttaaacttga atgggattat atgaaagatg tctttgatta tgatatatat
     9241 cttaaagata agttgtcagg agaatcattt aagaaaatta aatctataac tataaatgaa
     9301 agtgatttag gtaagtctaa caaggtatat gcaactatag aagtagatat gaagggtaat
     9361 caaaatatag attttgatat ttatgtcata gggaaaaagg attcagatac aactaagcca
     9421 agtaatgtaa taaatattac caatggaaat ggaaattcaa ataatgataa taatattaaa
     9481 gatatgaggg tagaaaatat atcagctaat gttgatagag ataataatgt cattagagtt
     9541 aactggagta gtatatctca taaggactgt acttacagta tatatgtaaa aaataatact
     9601 aatggagatg gagaatttac tctatatcaa agtgatatac agggaaatga gcatactata
     9661 agtattcctg aaggagattt aaatctagat atttatgttg ttgcaaatta taatggaaat
     9721 agctctcaaa atagtgatgt tataaatgta agatagattt gtatacaaat tgaaagtgtt
     9781 gagtttattt aaatattaaa ttaagaaaaa ggatagtata gggtaattat actatccttt
     9841 tttgttacaa tatataggtt taaaatctct agtaatataa attataaaaa gtcttattta
     9901 cacaaatcta tagatatttt ttttattaat caatattgtc ttaaattgaa cttagtgcgg
     9961 cttctattgc atgtattact gtcgtatcaa atagtggaat agaagtgtca ttttgtttaa
    10021 ttaaaagacc tatttctgta catcctaata taacagcctc tgcaccttgt ttgccaagct
    10081 tatctataat agataagaaa gctttttttg aagattcaga tataattcct aagcaaagtt
    10141 cattaaatat tgtgtcattt actattatac ggtcttcttc atttggaatt aagacttcaa
    10201 ttccattatt aataattaca tttttataaa aatcttgttc cattgtatat ttagtaccta
    10261 ataaggccac tttttttatt tctttttccc ttaataccgt agcagttaca tctgctatgt
    10321 gaagtaatgg aatatgtata ctttcttgta ttttatctga taccttatgc atagtattag
    10381 tacagataac aataaaatct gcccctgcct cttgtaattt aattgcagca tcagctaaaa
    10441 ttttggcgct tttttcccaa tttccactag attggcattc ttcaatttct tgaaaatcta
    10501 cactgtaaag tatacattta gaagagtgta gtccacctaa tctttctttt attactgtat
    10561 taataacttg ataatatgta atggtacttt cccaactcat accaccaatt aagcctatag
    10621 tcttcatatt atacctctct tatttattta ttttgaaata gctatttaca aatacaagta
    10681 tagcatctaa aatagaagca tacaatgata aaattgtatg gagtacaatt ataattaaga
    10741 ggagtaatta tatgccaata aattcttttg aaaactatcc tatgacatgg aaaccaaaat
    10801 tagataaccg aaaaccacca atatataaaa ctttagcaat gttattagaa gaagatatta
    10861 aaagaggaaa tttaaatcct ggagataaac tgcctccaca acgggaatta gcagattttc
    10921 tagatttaaa cttaagtaca ataacacgag catttaaact ttgcgaagaa aagggactaa
    10981 tatgtgcaaa agtgggaaag ggaacattca tttcttctga tgtaaatgta agtaatacat
    11041 tattatatca aacggaatca aaagacataa ttgaattagg gacagttcat cctccgtatg
    11101 agcaaaatac atatattatt gactttatta aaaatgtact aaaacaacca gaaatggata
    11161 ggtttttaca atacatgtct ccatctggca catatatgca aaaaaagtca attgcaaaat
    11221 ggatagaaag aaataatgta tacaccagtg aagaaaatat attattatct acaggagggc
    11281 aaaatgctat atgtgcaact ttactaggat tatttaaagc tggtgataga attgctacag
    11341 attctttaag tttttctgga attaagtcaa tagctaaaat gataggtatt caattagtcc
    11401 caattcctca agaaaataat gaaatatcga ttgaatatct tgaaagttat tgtaaaaatg
    11461 aaaatataaa aggcatatat cttataccag attatcataa tccaacgaca catactatgt
    11521 ctgatttatc tcgtaaaaag atagccaaga tagcaaagca atataatata ataattattg
    11581 aagatgctat aaatagcatt tttagagatg gtatacaaac tcccatattt tctttagcta
    11641 gtgataatac tatatatatt tttagtacat ctaaattttt atgtgctggt cttagagtag
    11701 catttgtagt tgctccaaaa cgatatatag aaaatttaga aaatgcacta tataatatga
    11761 atctaatggt ttctccattt acagcagaaa tagtacaccg tttattatat tctcctatta
    11821 tagataaaat tatagaagag aaaagagatg caattataga aagaaatgaa gttgctgata
    11881 aagttttgtc agattataat ttgattggtg ataagaattg cagttttcgt tggttacttc
    11941 ttcctgatga attggatgga aaatcatttg aaatttctgc aaaaaatcta ggagttcaag
    12001 tatattgtgc tgaacgattt tcagtaggaa actctacagt tccaaaagca gttcgtattt
    12061 gtgtaacagc tccaaaagat gtagaagaat tagaaaaagg gcttaatata ataaagtcac
    12121 ttcttaagag ttaaatttat tttactatta tttaattaaa taaataaaaa ggcggagaaa
    12181 tttattataa taaacctcc
//
LOCUS       DAYASV010000112        12170 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYASV010000112 DAYASV010000000
VERSION     DAYASV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.113"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..341
                     /locus_tag="ACGI68_002906"
     CDS             <1..341
                     /locus_tag="ACGI68_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139333.1"
                     /translation="SDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVED
                     TGCGIKKEDLNRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYT
                     KFTITFKCDL"
     gene            453..1682
                     /locus_tag="ACGI68_002907"
     CDS             453..1682
                     /locus_tag="ACGI68_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0139334.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALSSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            1690..2364
                     /locus_tag="ACGI68_002908"
     CDS             1690..2364
                     /locus_tag="ACGI68_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139335.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            2367..3599
                     /locus_tag="ACGI68_002909"
     CDS             2367..3599
                     /locus_tag="ACGI68_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139336.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFTATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(3885..5150)
                     /locus_tag="ACGI68_002910"
     CDS             complement(3885..5150)
                     /locus_tag="ACGI68_002910"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFH0139337.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(5203..6351)
                     /locus_tag="ACGI68_002911"
     CDS             complement(5203..6351)
                     /locus_tag="ACGI68_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0139338.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNRVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(6442..6987)
                     /locus_tag="ACGI68_002912"
     CDS             complement(6442..6987)
                     /locus_tag="ACGI68_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HFH0139339.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(7210..8154)
                     /locus_tag="ACGI68_002913"
     CDS             complement(7210..8154)
                     /locus_tag="ACGI68_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0139340.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            8540..9421
                     /locus_tag="ACGI68_002914"
     CDS             8540..9421
                     /locus_tag="ACGI68_002914"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0139341.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(9482..10690)
                     /gene="tyrS"
                     /locus_tag="ACGI68_002915"
     CDS             complement(9482..10690)
                     /gene="tyrS"
                     /locus_tag="ACGI68_002915"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFH0139342.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(10763..11015)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11167..12135)
                     /locus_tag="ACGI68_002916"
     CDS             complement(11167..12135)
                     /locus_tag="ACGI68_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HFH0139343.1"
                     /translation="MNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDFIVPVFFC
                     IICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINKPTMVMDD
                     LVYGSVFGLIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLMIALFPFG
                     MVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRSLNQFLCCSKKAFYICVSYLAIFE
                     VLKLLNVKVDSVYLKTIFISITYISLYFIIRDSCMSLQNFIIAKFRKLLYARILFLII
                     ILLLFFIFDVTVIGLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
BASE COUNT         4253 a         2004 c         1531 g         4382 t
ORIGIN      
        1 tatctgatga aaaatggcta tacttcatat tcgagcaatt attgagtaat tccctaaaat
       61 actcctcatt aggagataat atatctatct attgcaaaag aaccaaaaat tatttacaat
      121 taagagtgga agatactggt tgtggtataa aaaaggaaga tttaaatcga atattcaata
      181 agggatttac aggtaataat ggaagaaaca attctaaatc tacaggtctt gggctttatt
      241 tagtaaaaga actttgtgat aagcttggtc ataaaattga tgtagattca gagtacaacc
      301 aatatacaaa atttactatt acattcaaat gtgatttata gtatcttatt attgatagtt
      361 tattttcatt aacttatatt caacttctta tagtaattct atatgtacat cccagtatat
      421 aattaagtag tttttataaa aaggaggaag acatgtcttt tctaaaaaaa tttaaaaata
      481 aaagatttag aaataacagt tttaaaagta acaactttag aaatagcagt tttggaagta
      541 gtggcttcaa gtctaaaaat aaaggtttta aaaacaatcc ctttaaaaat aagagattca
      601 aaatatatgc aggaatagca gtaatcatac ttttaattct aggagttctt gcttatgttg
      661 attcgaaaaa cactgaatta caagctaatg aaaattttat cgaaacatat actattcctg
      721 aaaatgaaaa aatatttata aatgggatgg ttgttcctaa gcaaactaag gacttcaata
      781 tatctggaga ttatgagtta agtgatgtta atgttacaaa tggtcaaaaa gtaaatcaag
      841 gagaccttct ttttactgct aaaaatccaa ctataatagc agaaatagat agtttaaaat
      901 cacaacttag tcaatacaaa aaacaaaaaa tatcactttc tgatattgct gaaaataaag
      961 atgctattgc ttctataaat gcacagataa ctgctttaag ctcccagata gcttctctgg
     1021 ataaaaaagc ttatgacaga gttactgctc cttttgatgg cacagtatac ttgaatgacc
     1081 aaactggaaa tccagaccaa ccagtttcat ttatgactat tcaaggtcta gagttttata
     1141 tgaaaggaca agcaagtgaa caagatttac ctaaaatgaa gattgaccaa atggtcaata
     1201 tactagtttt ttcaactgac caaaagctta ctggtagaat aagttttata tctgataaac
     1261 catctactcc aaatactgaa atgggagctc agcagaatac actatcttat tatgatataa
     1321 acatagcatt tgataatcaa gaaggattag taaatggatt ccatcttcaa gcatcacttg
     1381 aagtagctaa ttcaagcttt aaaataccag catcttgtgt acttaaagac aacaaacact
     1441 catatgtatt taaggattta gatggtatat tgaaaaagca aattgtagat gtagcaagtc
     1501 aaaatgatga ttttgctgta gttagaggcg gtcttgaaca aggcgatata ataataaaac
     1561 accctactaa agaaatgaaa gaaggagacc cagttcaagg cgatggcgta actgctggtt
     1621 ctaataatgg agatactact ccaaacaaaa aagtagaaga agttactatg gatgtgaatt
     1681 aatttacata tgttaattaa attagaaaat attcaaaaat attacaaagt agggaaagat
     1741 gaattacatg ttttaaaatc cttaaattta gaaattgaat ctggagaatt tgtcatgata
     1801 atgggtaagt ctggtagtgg taaaactact ttacttaata tcttaggttt tctagatgta
     1861 tttgatgaag gaagatatat atttgatgga acagatgtaa ctaatttgag tgaaaatgaa
     1921 cgttctgttt ttagaaatat taatattgga tttgttttcc aacaatttaa tttaatagaa
     1981 actctaaatg tatatcaaaa tgtagagttg cctctaattt acaataaagc actaaaaaaa
     2041 tctaatagag aagaaattgt taaagataaa ttaagttctg ttgggctttt ggacaagctt
     2101 aagcagaaac cacttcaatt atctggtggt caacaacagc gtgtagccat tgcacgttgc
     2161 ctagcaaatg accctcagat aatctttgct gacgaaccga ctggggcatt agatagtgaa
     2221 actagcagag aaattatgga gcttttaaca aggctcaaca aacaaggtaa gactataata
     2281 atggttacac atgatcaaga tttaactaag tatgcaacta aggttatacg acttaaagat
     2341 ggtgtgttta cttcggaggt gtaatcatga gtttattaaa aaattctttg gccaatttaa
     2401 aaggtcacaa gcttcgtgta tttgtagctt tactctggat tataatagga ataacttcag
     2461 taatacttgt tagttctata ggtaatggat ttcaaaaaga aattaagaaa tctgtaaaca
     2521 atgtaaaccc taataaaact actatttcgt ttgaatcagc cgataacaca ggtcttactg
     2581 atgatatgag tattttttta aaaccattta atgcaaaaga tttagaagaa ttatcatttg
     2641 ttgaaggagt agaaagaata gctccttcaa gagatgggtt taaccttgat tcagtttact
     2701 cttcacaagc atcttttgat aaaaaaacta cgtatgttga tgttggacca gtaaaaaaag
     2761 attctaaaat aaatttaatt tgtggtagag atttttcttt agatgatgaa aaaagaaaag
     2821 taattttact tacattacaa agtacaagtg aaatttttga aaatccagaa gatgccttag
     2881 gtcatggtat caacataaat ggaactatat ttgaaataat aggggtatta gatgattctc
     2941 agcaaaacca agctggagga ttctttggag gttaccaaga tatgcaattt actacttccc
     3001 tagtacctaa aaaggctttt gacactttaa tgagtcaaaa ttcttattca aatgaaattt
     3061 accagttaga cttagtttct tctaaaggct ataatgtaaa tgaagttgca aacaatgtta
     3121 tagctaaatt atatgagatg caccctggta tcaatggttc ttatactaca ccagacccaa
     3181 ctgaacagac agcttatctt gaaagcatta attccaatgt aaacaaatat gtatctataa
     3241 taactgtagt tgcaatgttt gttggtggta ttggtgtaat gaatataatg tatgtatctg
     3301 ttatggaaag acaaagagaa attggtataa gacgtgctat tggagcaaaa cctaggtcta
     3361 tattatttca atttttagtt gaagctgttt ttataacagt atgtggtgga attttaggaa
     3421 ctatagttgg atttactgct actaactatg tttctaaata tataggtttt gaagctatac
     3481 ctagcttaaa tagcttattt tatgcaattg tagctactat attgactgga gttgtatttg
     3541 gtctgattcc agcctttaaa gcctctaaac tagaccctat aaaggcaatt tataagtaga
     3601 aattgtataa agattttcga tttttagatt agtttatata aaacatactt aaatatataa
     3661 cgcactatta tatatgatag attaggaact taatctttcc aaatataata gtgcgttctt
     3721 tcataaaaag gattctcttc cttactatat ctaaattacc aagatgttat gttctaattg
     3781 ttcaaactaa tacttccaat tttgattaat caatctcatt ctcctaatat acaatctttt
     3841 actttcaatc attgtacact ccgcctaaaa aaaataatct taaattaggc gttatataca
     3901 atctccccat tacatattgt ataattaacc tttccatata aagatttacc tttaaatggt
     3961 gaattatcag ctttagaaac aaagctttca actgtccatt cttcttctgg attgaatata
     4021 acaatgtctg ctacagcccc ttcttctata aatccaatat ttaaattgta caactttgca
     4081 ggatttatag acattttttc tattaactgc atcatactaa gatgattttt atgcaccaaa
     4141 tttgttattc caagagctaa agaagtttca agcccaataa ttccacttgg agcttttata
     4201 aactctctat ctttttcttc tttactatga ggtgcatggt ccgttgctat tatttcaata
     4261 gtaccatcat ttaatccctc tataatctta tctctgtccc atttacttct tagaggtgga
     4321 ttcatttttg catttgttcc aaactccaat acatcttcct cagtcaatgt aaagtgatgt
     4381 ggtgaagctt ctgcaactac atttgcacct aagtattttg caaatctaac catatcaaca
     4441 gatacacctg aacttatatg ttgtatatca actttagcac cagtctttag tgccaacata
     4501 caatctctgg ctaccataac atcttctgct acacttgatg caccttttaa tccaagttct
     4561 gaagctacct ttcctgcatt tacacctgga tttccaacta aagaagggtc ttcttcatga
     4621 aaactaagtg gtacatccaa ctcttttgcc ttaatcattg cctccataat tagattgcta
     4681 tccattaaag gtaatccatc atcagtaaac cctggtacac cagctttttt aagagcttcc
     4741 atatcaacta actctttacc ttcaaaacct ttagttatag ctgctgcttg taaaacattt
     4801 atacatgcat tttttgactt ttctttaaca taattaaaag tatcttcatt atctacaata
     4861 ggattagtat ttgccataca tataacagtt gtaaatccac ctctagcagc agatttagct
     4921 ccactttcta tatcttcttt atatgtaaac ccagggtctc taaagtgtac atgtacatcc
     4981 actaaacctg gagcaactac attaccacta gcatctatta ttttttcata ttcatcactt
     5041 tcatcaaact tgcctatttt ataaatttta ttttctttta taattaagtc aatattctca
     5101 tctctttgac ttttagggtc tattactcta ccatttttaa ttaaaatcat attatttctc
     5161 ctttatattt ttaatttata tagactcata taatattata cattatttta catacttaaa
     5221 tacaaaatta cgtttgacat caatagcctt tttaggacaa agttcatggc aacagtaaca
     5281 tcttatacat ttacttaagt ctataatagc acccttagtc cctatctcta tagtggatgc
     5341 aggacaagct tccttgcatt ttccacattt tatgcaatct tgaaatctta ccacaggctt
     5401 tggtgttata agctttgaaa taggttcatt taaaaatttt ggtagcttta cagaaaacaa
     5461 tcttaaatcc ttacttacat ttggaatctt gtaatttttt ataattaaat cttttatatc
     5521 ttcacctaaa atttcaatat ctgaaaaatc ttcttgtata tatcctctct taatagcacc
     5581 tctaattgta gcaactctat ttggaagtaa acttataatc ttacatgcaa caacatctat
     5641 agcatatgga ttttgtgatg ctaataaaac gcctatttct cgagtagtac cagctgaagg
     5701 tccatcacct tccataccta ctataccatc cataattgtc aatgttggtt ttatataatc
     5761 acaaatatct aaaagtacat tttcgcaaaa caactcttct gtaggaaatc tataatgtat
     5821 ctctgctttt ttcaatccag ctatgcatcc atataagttt tttgtaccac cagtaaaagt
     5881 tgccattata tgagtcttta atttacaaag atttattata taatctgaat ctactattgg
     5941 tttaataata tccatatatt ttagaacatg tgctttttca ctatgtatct taacatctga
     6001 aacatcataa tttaacttaa tgcccagttc atttgctact tcttcaattc cagatgcctt
     6061 ataaatgctc ttaagagaac tttgattata tggaccacca gggctatctg ctattgtaat
     6121 atcacaattt aactctaaaa gtttttcaca tacaactttt acaaccatag gatgtgtagt
     6181 tgtagcttct tcaggctttt ttttcattaa taaatttggt tttataagta ctttgcttcc
     6241 ttttttaaca aacttatcta taccaccaat atcctctaaa tttttttcaa tagataactt
     6301 tacttcttca taattgtaag ttttacaact cctaacagat accgacttca tattatctcc
     6361 atccttctat cacaacagtt tatttgtata atttaaaaaa ggagaaacca aagtttctcc
     6421 ttttaatcta tgttttaatt attattttcc aaaatgtctg tttaataatc ctaagaatgc
     6481 tttaccatgt ctagcttcgt ctttacacat ttcatgtact gtatcatgta tagcatctaa
     6541 tcctaattct ttagctctct tagctaattt taattttcca tcagttgcac catactcagc
     6601 atcaactcta actcttaagt tttcttttgt atctgcaact acaacttctc caagaagttc
     6661 tgcaaattta gcagcatgtt cagcctcttc aaaagctatt ctcttatacg cttcagctac
     6721 ttctggataa ccttctctat cagcttgtct actcattgct aaatacattc ctacttctgt
     6781 acactcacca gtaaagttgg ctcttaatcc ttcgattatt tctgcatcta caccttgagc
     6841 tactcctatt ctatgttcat cagcccaaac catttcgcct ttcatttctt caaatttatc
     6901 agctccaact ttacatactg gacattgtgc tggtgcagca tctccttcat gtatatatcc
     6961 acatactgta caaacaaatt ttttcataat taatttcctc ccaattttat ataattaaat
     7021 ttttattttt aagtttaata acttgctaca agaatatata taccccagac tttatagctt
     7081 aaacactaaa aataaaattt atttcctaat taattttatc actttgaaac accagtatca
     7141 accaagtttt gatgtcaaat ttctacataa tacataattt atttgattaa ttaggaaata
     7201 gttgtcatat taaatacttt ctaatgaaga ctcctctgaa tctttaagtt tcactttttt
     7261 gctctttaaa aaagataatt tagtttctgc agttatgatt gcaacaaata tcaagatact
     7321 accaataacc aattttgctg taataagctc ctttaaaatt attatagaaa atattgtacc
     7381 aaatacagcc tctgtggata atattatagc tgttctagtt ccatcaactc ttttttgaca
     7441 tatagtctga aataaaaatc caattgtagt actaaataca gctagataca tagttcccat
     7501 gtaaccagat aattcagctt ccatctttaa ttgacctgca aatgtttgaa ctacaacaga
     7561 cattaaaaac gctactgtaa attgaatagc tgtcaatgcc attgggttgt tatcttttgc
     7621 aaactcactt gtaaagaata tatgaaatgc aaatccgaat gaacatataa gtgttagaaa
     7681 atctccaaaa ttaatagaga aatcagcttc taaagaaagt atcccaattc ctataagagc
     7741 taccaaacta cttattattc ctattttatc aagttttctt ctataaagta taaatcctat
     7801 gaatggaact attacaacat tagctgctgt tataaacgca tttttagatg gtgtagtgta
     7861 cataagtcca atagtttgta ctgtgaatgc tagaaataaa aatattccta aaatccctcc
     7921 tgcttttaat aattttttac ccatattagc acgaatctgt ttaaaaaaca gtaaattcat
     7981 aattatagct gccaagaaaa atcttaaagt tatgatttgt agaggtgtaa gcccaccgtc
     8041 taatgcaaat tgggttgcta caaagccact tccccatata attgctataa atattagtcc
     8101 tatttctcca aagtattttt tcaataaagc cccctccttt ttattttttt gcaaaattat
     8161 aaacccccta aacagcaaga gagtttttct ataagcatta ctataaatta atatagtaaa
     8221 ttttttcagt attttaaatc taaatttaca tacattttga gctcaaattt atatccacta
     8281 tttaacagta atatattaat cattttttgt aaacaccttt ttaataaatt tttatatttt
     8341 ttatattttt ttacctatac taaatttttt tatagatatt tttaatatat atgatacttt
     8401 aatattatgc aaataatgtc atataagtta cactttagtt atcttagtta caactttgtc
     8461 ggtttacttt ttctactttc ttcgatataa ttataatata tttttgatac aattcgacaa
     8521 aagggggaat gctgtaatta tggtaaaaaa aagaaaaaag aaaactgtta cacttactat
     8581 acttgttgta atagttattg gtattttttc tatggcatgg gcggacatgt ctagaaaaaa
     8641 agaaatgaaa gaaactcaag aaagacttaa agctgaaaga ttagaaaaag aaagaattga
     8701 aaaagaaaaa aatgaaccta ttattggtgc aaaaaaagaa gctgtaaaag aatatggata
     8761 tgacgcaaaa atagtttggg acaaacttaa caaatacgac tattcaaaca atggagaaaa
     8821 gatagtcttt cttacatttg atgatggacc atctactact aatactccac aagttctaga
     8881 tatcttgaaa agacatggtg ttagaggaac tttctttatt aagggtgatt ctcttgaaag
     8941 aaaaggagct aatgaaattt taaaaagaac atttgacgaa ggaaatgcaa tagcccatca
     9001 ctcttatact catgactata aaaaactata tcctaacaga agcctaaatc tcgatacatt
     9061 cgtaaatgaa cttaacaaaa ctgatgaagc tatgaaaaaa gtcttaggaa agaatttttc
     9121 ctcaaatgta gttagatgcc caggcgggta tatgtcatgg aaaaatatgg aacctctagg
     9181 aaactactta aaagagaaaa acatggcatc aattgattgg aacgctttaa atgctgatgc
     9241 agaaggtaaa aagaaaaatg cacaagaatt atttgaacat gcaaagaaat cttctgaagg
     9301 caaagaaatg gttgttcttt taatgcatga tacttatgga aaacaagaaa cagtaaatgc
     9361 attggaccaa attataacat attttaaaga caatggttat caatttaaaa ttttaatata
     9421 ataaaaaagc aagtttattg aatgtacatt ttatactcaa tagacttgct tttactttac
     9481 tctattttac tagctcgatt tttacaaatt ttttctttcc tatttgaaca actaattcac
     9541 tctcttttac aatagtgctt aaatcctcta ctttttcacc atttaccttt actccacctt
     9601 gagaagctaa tcttctaact tcactcttac tcttaacaat ctcattagaa actagtactt
     9661 cgattaagtc aaatccatct tccttaactt gaattgtttg tatatcttca ggtatttgac
     9721 ctttttggaa tacagattta aatctttctt cagcttcttt agcagattct tctccatggt
     9781 ataatgttac tatttctctt gctaagttca tctttatatc tcttggattt actgaaccct
     9841 ctttcaactg tgattctatt ttattaactt catcaggatg aacatctgtc actaaattgt
     9901 agtatttaat tataagttca tcaggaattt ccatagattt ttggtacatt attcccgctt
     9961 cttcatctat tccaatatag ttacctaaac tcttactcat tttttcttta ccatctaatc
    10021 cttctattaa aggcatcatt attactattt gagactccat accaaattct ctttgtaatg
    10081 atcttcccat tagtaaattg aatctttggt cagttccacc aagttctata tcagcttcta
    10141 atgctataga gtcatatgct tgcattaatg ggtagaagaa ctcatgtact gatattggca
    10201 tttgtccttc atatctcttt ttaaaatctt ctctttctaa cattctcgca acagtaatag
    10261 ttgctgctaa ttttattaca tcttcaaaat ttaacttagc taaccactcg ctattaaatc
    10321 ttacaatagt tttttcttta tctaaaactt tgaaaatttg ttcttcataa gtttttgcat
    10381 ttgcaagaac ttgctctgtt gttaaagctt ttctagcttt agattttcca gttgggtctc
    10441 caatttttcc tgtaaaatct ccaattatta ttactatttg atgacctaaa tcttgtaatt
    10501 gtttcatctt tctaagaact actgtatgcc ctaaatgtat atctggagca cttgggtcta
    10561 gacccaattt tactatcata ggctttcctt ctttttctga tttaataagc ttttccctta
    10621 actctttctc atctattaaa tcatctacac ctttcataat tattcgcatt tgctcgtcta
    10681 tcgatttcat ttttcattcc tcctaaaata tttatataat ctataatttt tatttttata
    10741 ataaaaaaag ctttcatcct cttaaaagga cgaaagcatt gcttacgtgt taccacctta
    10801 gttcatcaat atctcacgat attaatctct tagagtacaa ttacatatac tcgtgtacta
    10861 taacgtgtac taatccgata tctcttaatg gttgttatct ttcagagata gtgctctgag
    10921 atgtattcaa actaaatcaa tttattccat ttcaccaaac tggaactctc tgttaaataa
    10981 aatctagccc tacttcttct cttcatagca tttatatatt cagattattt catcaattac
    11041 aagaaatttt tcttaactta ctcataatat attattattt ttatattttg tcaacctttt
    11101 ttattattat atacatgttt ttttacatta tactactttt ttatattttt taccaatacc
    11161 caaaatttat ttttcaatca aaagatttat ttgtttattg actatattac tctgactcaa
    11221 tcttatattt attttcttat ttaaaaatgc gtttataatt attaaaccaa taactgttac
    11281 atcaaatata aaaaacagca ataaaattat tatcaaaaat aaaattctag catataataa
    11341 ttttctaaac ttagctatta taaagttttg tagtgacata catgagtctc taatgataaa
    11401 atagagactt atataggtta ttgatataaa gattgttttt aaataaacag aatcaacttt
    11461 aacattcaac agttttagaa cttcaaaaat ggctagataa gaaacacata tgtaaaaagc
    11521 tttttttgag caacataaaa attggtttaa acttctaaaa ttcttaaata ttaaaaattt
    11581 ttttagagaa ttagaatcta aaatatgcaa tttattccct aaaattaaac ttaataaata
    11641 tatgcaaaac accataccaa aaggaaacaa tgctatcatt aaataataaa taaaatttgt
    11701 atacccatta aaataaatcc atttgctata tccttgaaat tctttcataa aactataacc
    11761 tattaatgtt gaagcatata tatcaataaa aaccattaca ataagcccaa atactgaacc
    11821 atatactaaa tcatccataa ccattgtagg cttatttatt agatagccac acattatacc
    11881 aagtaataca ctctgacttg cccaaatagc agttcctaga tttccaagta ccagagaaat
    11941 tattaaaagc gaagtaattc cagataaaat ggtatacttt aaatcacatt tcaagcagat
    12001 tatacaaaaa aatactggca ctataaaatc taagtaaata gcatatccaa atccagtact
    12061 cacagctata attgttacta ctatgaataa agatgacaat aaagctgctt cagttaattt
    12121 tttagtagta ttcaaaacac tgcctccata aatcataaat tttctaacta
//
LOCUS       DAYASV010000113        12160 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYASV010000113 DAYASV010000000
VERSION     DAYASV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.114"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            164..952
                     /locus_tag="ACGI68_002917"
     CDS             164..952
                     /locus_tag="ACGI68_002917"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFH0139344.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1423..5139
                     /gene="rpoB"
                     /locus_tag="ACGI68_002918"
     CDS             1423..5139
                     /gene="rpoB"
                     /locus_tag="ACGI68_002918"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFH0139345.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIIKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5181..8666
                     /gene="rpoC"
                     /locus_tag="ACGI68_002919"
     CDS             5181..8666
                     /gene="rpoC"
                     /locus_tag="ACGI68_002919"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFH0139346.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8955..9377
                     /gene="rpsL"
                     /locus_tag="ACGI68_002920"
     CDS             8955..9377
                     /gene="rpsL"
                     /locus_tag="ACGI68_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFH0139347.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9501..9971
                     /gene="rpsG"
                     /locus_tag="ACGI68_002921"
     CDS             9501..9971
                     /gene="rpsG"
                     /locus_tag="ACGI68_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFH0139348.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10020..12086
                     /gene="fusA"
                     /locus_tag="ACGI68_002922"
     CDS             10020..12086
                     /gene="fusA"
                     /locus_tag="ACGI68_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFH0139349.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4711 a         1535 c         2497 g         3417 t
ORIGIN      
        1 taaaatgtaa aattttacat tttagtttaa aaaaatacac gagtgtttat aaataaaatg
       61 taataaataa ttaattaaca aatataaatt ttaaaataag ttagatattt agaaaaatcg
      121 aaatttgtag gatttaaagg acatattcaa ggaggagtta ataatggaaa aattacaagg
      181 aaaaattgca gtagttactg cagcaacaaa aggtattgga ttagcatcag cagagatatt
      241 agcaaaaaat ggagcaactg tgtacttagc agctcgttca gaagaattag ctaatgaagt
      301 tataaataaa ataagtgcag aaggtggttg tgctaagttt gtttacttta atgctcgtga
      361 agaagagacc tttacctcaa tgatagaaga ggtagttaaa aaagaaggta agatagatat
      421 acttgtaaac aattttggtt caacaaaccc ttctcttgac aaagaccttg taactgggga
      481 tacagataat ttctttgaca cagtaaatac taatttaaaa agtgtgtatt taccatgtaa
      541 agcagcaatt cctcatatga taaagaatgg aaaaggaagt atagtaaata tatcaagtat
      601 aggttcagta ttacctgatt tatctagaat agcttactgt gtatcaaaag cagcaatcaa
      661 ctcattaact caaaacatag ctacacaata tgctaaagac aatgttagat gtaatgctgt
      721 acttccaggg cttattgcaa ctaaagcagc attagacaat atgtctccag agttcataaa
      781 agaattttta aagcatgttc ctttaaatcg tataggggaa ccagatgata tagcaaaagc
      841 tgttctattc tatgctagtg atgactcatc atttataaca ggggatttac ttgaagttgc
      901 aggaggattt ggtttaccta ctccacaatt tgcagataat atattaggat aattaaatta
      961 ctaatatgca atgaaagctt atgcattagt tatagtagaa atgatatttt tataaaggcc
     1021 atagatataa tttatattgg aaaggtttga ggttcaaaat tccaagttaa atttatttat
     1081 ggtctttatt ttgtatattt taataaattg tatatatttg tataaatgta attatttaca
     1141 atcattttaa tatggaatta taattaaata ggggggatgg tacttgtata aaatttttta
     1201 tagaaaatta gacctataaa tgaaaaatat ggattttata ttggaaaatt gtacataata
     1261 cataatatat gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa
     1321 gataaaaaat atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat
     1381 gaactattgt tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg
     1441 ataggtaaaa gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat
     1501 cttatagaaa ttcaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg
     1561 tttgatgata tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac
     1621 tattctctag atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca
     1681 tattgtgcac ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa
     1741 gaacaagaag tgtttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata
     1801 aatggagcag aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg
     1861 gaagaaagag ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga
     1921 gcatggttag aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca
     1981 agaaaacaac cagttacagt tttacttaga gctttaggaa taggaacaga tgcagagata
     2041 attgaccttt taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag
     2101 acagtagaag aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact
     2161 gtagaaagtg catcatcttt attaaatgct ttattctttg acccaaaaag atatgaccta
     2221 gcaaaagttg gtagatataa attcaataag aaacttgctt tatgttatag aataatgaat
     2281 aaaatttcag cagaagacat aataaatcca gaaactggag aagtatttgt aaaagctgga
     2341 gaaaaaataa gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat
     2401 ctactaatgg atgatgataa aaaagttaga gttataggaa ataattttgt ggatattaag
     2461 tctcacattg attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact
     2521 ttaaaagaaa ttttagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca
     2581 agaattaaag aacttatacc aaaacatata ttattagatg atataatagc ttcaataagt
     2641 tatgagttca atatattcta taatatagga aatattgatg atatagatca cctaggaaac
     2701 agaagaataa gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga
     2761 atggaaagag ttataaaaga aagaatgacg gttcaagata tggaagctat aacgcctcaa
     2821 gcgttagtta acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag
     2881 ttatctcagt tcatggatca aacaaatcct ctatctgagt taacgcataa gagaagatta
     2941 tcagcccttg ggcctggagg tctttcaaga gaaagagctg gattcgaagt gcgtgacgtt
     3001 caccattcac actatggtag aatgtgtcca atagagactc cagagggacc aaatatcggt
     3061 cttataaact ctctaggaac ttatgcaaaa ataaatgaat ttggatttat agaatcacca
     3121 tatagaaaat ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct
     3181 gacgaagaag atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa
     3241 ttcgtaaacc atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa
     3301 agtagagtag attacatgga tatatctcct aagcaggttg tatctgttgc cactgctatg
     3361 atacctttct tagaaaatga tgatgccaac cgtgccctta tgggagcgaa catgcagcgt
     3421 caggcagtgc ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga
     3481 gcagcaaaag actctggagc agttgttgtt gctagaaatt ctggtatagc agaaagagta
     3541 acagcagatg aaataattat aaaaagagaa gatggaaaca gagatagata taatctactt
     3601 aagtttaaac gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt
     3661 gaccagataa taaaaggtga tgttatagca gatggtccag caactgattt aggagaagtt
     3721 gcacttggaa gaaactgtct tatagcattc atgacttggg aaggttataa ctacgaggat
     3781 gctatattaa taaatgaaag attagttaaa gaagatagat tatcaacaat tcatatagaa
     3841 gaatatgaat gtgaagctag agatacaaaa ctagggccag aggaaataac tagagatata
     3901 cctaatgttg gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt
     3961 gcagaagtag actcaggtga tatattagtt ggtaaagtaa ctccaaaagg agaaactgaa
     4021 cttactgctg aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga
     4081 gatacttcac ttaaagtgcc tcatggtgaa tctggtataa tagttgatgt aaaagtattt
     4141 actagagaaa atggagatga tttatctcca ggagtaaatg aattagtaag atgctatata
     4201 gctaaaaaga gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg
     4261 gttatctcaa gagtattacc tgaagaagat atgccattca tggaaaatgg aacaccacta
     4321 gacataatac ttaacccaca aggtatacca tcacgtatga acattggtca ggttttagaa
     4381 gttcatttag gacttgctgc taagacatta ggatggtatg tagcaacatc agtattcgat
     4441 ggtgctaatg agtatgacat aatggatgct cttgaagaag ctgggtatcc tagagatggt
     4501 aaattaactt tatatgatgg tagaacaggt gagtcttttg acaacagaat aactgttgga
     4561 tatatgtact acttgaaact acatcactta gtagatgaaa aactacatgc aagaagtact
     4621 ggaccatact cattagttac tcaacagcca ctaggtggta aagcacaatt tggtggacaa
     4681 agatttggag agatggaggt ttgggctctg gaagcatatg gagctgctca catacttcaa
     4741 gaaatactta ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata
     4801 gttaaaggtg aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa
     4861 gaacttcaaa gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa
     4921 gtaagagaat ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat
     4981 catatgggag aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa
     5041 aatgcagaag atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa
     5101 gaagaaatag attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca
     5161 aatagaaggg agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat
     5221 tggcttctcc agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta
     5281 taaattaccg tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac
     5341 cacaaaaaga ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag
     5401 tttgtgatag atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac
     5461 atatagagct agctgctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa
     5521 tgggactttt acttgatatg tcaccaagat cgttagaaaa aatattatac tttgcctcat
     5581 atgtggtagt tgatccagga gagactggat taaacgaaaa acaattgctt acagaaaaag
     5641 aatacagaac tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgctgaag
     5701 ctgtaaaaac attactacaa aatatagatt tagagcaaca aagtaaagac ctaagagcgg
     5761 agttaaaaga tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag
     5821 aggcatttaa aaagtctgga aataaaccag aatggatgat tttagatgca ataccagtaa
     5881 taccaccaga tttaagacca atggtacaac ttgatggtgg aagatttgcg acttcagacc
     5941 taaatgattt atatagaaga gttataaata gaaacaacag acttaaaaga ttattagagc
     6001 ttggagctcc agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg
     6061 cattgataga taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta
     6121 aatctttatc agatatgtta aaaggtaagc aaggtcgttt ccgtcaaaac ttacttggta
     6181 agcgtgttga ctactcagga cgttctgtta tagttgttgg accagaactt aaattttatc
     6241 aatgtggact tccaaagaag atggcattag aattattcaa gccatttgtt atggataaat
     6301 tagttaaaga aggttatgca cataatataa aaagtgcgaa atctatagta gaaaaagtta
     6361 agccagaagt ttgggatgtt ttagaagatg ttataaaaag tcatccagtt cttcttaacc
     6421 gtgcaccgac tctacataga ttaggtatac aagcatttga accaatctta gttgaaggta
     6481 aggctataaa actacatcct cttgtatgta cagcttataa tgcagacttt gatggtgacc
     6541 aaatggcagt tcatgtacct ttatcagtag aagcacaagc agaagcaaga ttcttaatgc
     6601 tttctgtaaa taatatactt gctcctaaag atggttcacc tataactact ccatctcagg
     6661 atatggtttt aggttgctat tatctaacaa tagaagccca agatggagct aaaggaacag
     6721 gtatggtatt taaagacttt aatgaactat tacttgctta ttacaataaa tcagttcatc
     6781 tacatgcatt agtgaaactt aaggtaacac ttgaagatgg aagaagttca ttggttgaaa
     6841 gtactgttgg tagatttata tttaatgaaa atatacctca agacttaggt tttgtagata
     6901 gaaaagaaaa tccatttgca cttgaagttg atttcttagc agataaaaaa tctcttggta
     6961 aaataataga taaatgcttt agaaaacacg gaaatacaga aacagctgaa ttactagatt
     7021 atataaaagc tctaggattt aaatattcta cattaggtgg cataacagtt gctgttgatg
     7081 atatgagtgt cccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata
     7141 agtatgaaaa agcatacaga agaggtctaa tctctgatga agaaagatat gaaaaagtta
     7201 tagagacatg gacagaaaca actgataaag ttactgatgc tcttatgggt ggactagata
     7261 gattaaataa tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta
     7321 gacagctggc aggtatgcgt ggtcttatgg caaatgcatc tggtaaaaca gttgagatac
     7381 cagttaaatc taatttccgt gaaggtttat cagtactaga atactttaca tcttcacatg
     7441 gtgccagaaa aggtcttgcc gatacagcta tacgtacagc tgaatctgga tatttaacaa
     7501 gaagacttgt tgatgtaagt caagatgtta ttgtaaggga aatagattgt ggaacagaag
     7561 atactacaga gatttatgct ataaaagaag gaaatgaagt tatagaagag atatatgata
     7621 gaattgtagg aagatatact atagacccta tattaaatcc tgaaactgga gaagttatag
     7681 ttgaggctga ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggaattg
     7741 aaaaaattag gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt
     7801 gttatggtag aaacttggca acaggaaaag aagttaatat aggtgaagca gttggtataa
     7861 tagcagctca atctatcggt gagccgggta ctcagcttac aatgcgtaca ttccatactg
     7921 gaggagttgc aggagctgat ataactcaag gtctaccaag ggttgaagaa ttatttgaag
     7981 ctagaaaacc aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg
     8041 aaacaggaaa gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg
     8101 taattccata tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc
     8161 ctctaacaca aggatttata aatcctcatg atatagtaag agtaaatggt gttaagggag
     8221 ttcaagaata tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta
     8281 atgataagca tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc
     8341 caggagatac agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta
     8401 ataaagatgc tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta
     8461 taactaaagc atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa
     8521 caagagtatt aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag
     8581 aaaatgttat attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata
     8641 tagctgttga aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt
     8701 atttgtggtg ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag
     8761 ttcttagtga cttttagatg gcaataaatg agaactaaaa agcatcaagc tagaaaagtg
     8821 ctaatatcac atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca
     8881 gtcagtatga atattcatat tgatgtagtt ggcggtcact gataagatcg aaaatttgga
     8941 aggaggtgca gatgatgcca acaattaacc aattagttcg taaaagtaga aaagcgctag
     9001 agaagaaatc tactgctcca gcattacaaa aaggttacaa ctctttaaat aagaaagtaa
     9061 ctgacgctag tgccccacaa aaaagagggg tttgtacttc agtaaaaaca gttactccta
     9121 agaagcctaa ctcagcttta agaaaagttg ccagagttag attaactaat ggtatagaag
     9181 tttctgctta tataccagga gaaggacaca acttacaaga gcatagtgtt gttcttataa
     9241 gaggaggaag agtaaaagac cttccagggg ttagatacca tatactaaga ggtactctag
     9301 atacagcagg tgttgataag agaagacaat caagatctaa atacggtgcg aagagaccta
     9361 aagaagctaa aaagtaaaaa caattaatga ttgattatag ttcggtgctt taaatatata
     9421 tatatttacg gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa
     9481 taattaagga gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac
     9541 cagatcctat gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg
     9601 gaaaaaaagg aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga
     9661 caggagaaga agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag
     9721 aagtaaaagc aagaagagtt ggtggggcaa actaccaagt gccaatcgaa gttagacctg
     9781 aaagaagaca aacattaggt ttaagatggc tagtaaaata tactagagct cgtggagaaa
     9841 aaggaatggt tgaaaagcta gctaaagaga taatggacgc agctaacaac actggagctt
     9901 cagttaagaa aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt
     9961 acagatggta ggatatatag atatcttata aaattgagga tatcataagg aggataacca
    10021 tggctagaaa gtttcctttg gaaagaacta ggaatatagg aatcatggct catatagatg
    10081 caggaaaaac tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag
    10141 gagaaactca tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta
    10201 taacaataac ttctgccgct actactgctt catggaaaga ccatagaata aacataatag
    10261 atactcctgg acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgacg
    10321 gttcagttgc tgtattctgt gcaaaaggtg gggttgagcc tcaatcagaa aatgtatgga
    10381 gacaagctga aacttatgga gtacctagaa tagcttttgt aaataaaatg gatatattag
    10441 gtgcagattt ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc
    10501 ctatgcaatt accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa
    10561 tggatgctgt tatatacaaa gacgacctag gggttgaaat ggaagaaaca gatataccag
    10621 aagatatgaa agaattagct gctgagtgga gagaaaaatt agttgaatca gtagctgaaa
    10681 ctgatgaaga attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa
    10741 aagttgctat aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag
    10801 cttatagaaa caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcgc
    10861 caactgatat accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt
    10921 cttctgatga agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg
    10981 gaaaactagc gttcttcaga gtttattctg gaacattaga atcaggttct tatgttttaa
    11041 atgcaactaa gaataagaga gaaagaattg gtcgtatatt acaaatgcat gctaatacaa
    11101 gagaagaaat aactaaagtg tatgctggag atatagcagc agcagtagga ttaaaagata
    11161 ctactactgg agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat
    11221 tccctgagcc agttatatct gttgctatag aaccaagttc taaagcagct caagaaaaaa
    11281 tgggtatagc tcttcaaaaa ttagctgaag aagacccaac tttcacagtt aagactgacc
    11341 aagaaacagg acaaactata atatctggta tgggtgaatt acacttagag ataatagtag
    11401 atagattatt aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata
    11461 gagagactat aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta
    11521 gaggacaata cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca
    11581 aattcacaaa caaaactgtt ggtggatcag taccaaaaga atatgttggt ccagttgaca
    11641 tgggtataca aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg
    11701 ctgttgagtt atatgatggt tcttaccatg aggttgactc ttctgagatg gcgtttaaga
    11761 tggctggttc aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc
    11821 cttacttcaa agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact
    11881 taaactcaag aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa
    11941 atgcattcgt tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc
    12001 aaggtcgtgc aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg
    12061 ctaagaaaat agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat
    12121 aatcagctag atgagagata tctctcatct agcacaagat
//
LOCUS       DAYASV010000114        12115 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYASV010000114 DAYASV010000000
VERSION     DAYASV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.115"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            30..905
                     /locus_tag="ACGI68_002923"
     CDS             30..905
                     /locus_tag="ACGI68_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFH0139350.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDINTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            1264..2568
                     /locus_tag="ACGI68_002924"
     CDS             1264..2568
                     /locus_tag="ACGI68_002924"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/bile acid-CoA:amino acid
                     N-acyltransferase family protein"
                     /protein_id="HFH0139351.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKGIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLKALEIFEENNYPYNVELLTYNNMGHPLPIPYIMPLKETLCMS
                     MSGGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNNYY"
     gene            2752..4143
                     /locus_tag="ACGI68_002925"
     CDS             2752..4143
                     /locus_tag="ACGI68_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFH0139352.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            4320..4907
                     /locus_tag="ACGI68_002926"
     CDS             4320..4907
                     /locus_tag="ACGI68_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139353.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYRNSKSVSPYLLLPLSYIEFAQEETYLFKL
                     LFINDLDLMMKEANDFYKEIDNEKKARLFSESIGIDLECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKSDWNLSI"
     gene            4940..5731
                     /locus_tag="ACGI68_002927"
     CDS             4940..5731
                     /locus_tag="ACGI68_002927"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0139354.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(5912..8329)
                     /locus_tag="ACGI68_002928"
     CDS             complement(5912..8329)
                     /locus_tag="ACGI68_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0139355.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKDKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(8342..9007)
                     /locus_tag="ACGI68_002929"
     CDS             complement(8342..9007)
                     /locus_tag="ACGI68_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139356.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(9089..10120)
                     /locus_tag="ACGI68_002930"
     CDS             complement(9089..10120)
                     /locus_tag="ACGI68_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139357.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITDDLKLIALLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSRICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            complement(10110..10796)
                     /locus_tag="ACGI68_002931"
     CDS             complement(10110..10796)
                     /locus_tag="ACGI68_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139358.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(10954..12018)
                     /locus_tag="ACGI68_002932"
     CDS             complement(10954..12018)
                     /locus_tag="ACGI68_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HFH0139359.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
BASE COUNT         4393 a         1542 c         1732 g         4448 t
ORIGIN      
        1 gatgaaacta atggggaggt taaaactcaa tgattacttt tgatgtaaat ggagctatta
       61 tcagatttga tgatacaaaa aataattata ataagataag gaaaatcttt aaattatatg
      121 ctttggaaat atctaaaaac tttgagagag attgtttaaa tagttttcaa aatctaaagc
      181 aaatatctga tagaggttta agtttaggag aaaaatatat agaagagtct tttaagaaag
      241 gaattgagac tatagtaagt tttggaatta taactataga tatcaatacc tttaaaagtg
      301 tttattgtga aaaatattta gattttaaga gactttttaa taacttgaat aaaactttta
      361 ttataccaaa taaaaataaa aaaaatggcc acgcgagctt cttagaaata gaatcgacaa
      421 taaagaaaat gagcaattat ttatacaatg attgttttaa aattcattat gctgttgtag
      481 atgcccttat agaaaatcgt ataactgcgg tacattctta tattgatgaa gaatctataa
      541 aaaaatctaa tgccttattt aataattata aagatggttt tattgggaag gctgacgaat
      601 gcaaagtagt aaaacaaata ataactttaa atccatatag ggaagatgta tacgaatttt
      661 tggtaaaaga agatggagat tttagtaagg aaattgagag attgactgaa tttttaggct
      721 atgatataag agaatataaa gcgtcattaa tggatattta tataaatgag ttaatggaga
      781 gcgatgaaaa tgatgttgaa aatgcaaaag aaaagattga aaaatatgct agatatattg
      841 gatgtagtga tgagaattta tatgtagcaa gagttaatgc catatatact tttgcaaatg
      901 cataatttta ttaaacatta aatattagat gttcgattat ataaatttaa ataaaaaaat
      961 atccttatta ataacaaata gtttatatta aaaaccattc ttaaaatgag tggtttttta
     1021 ttttgaatat atatattcta aacctaatag cattcctatc tttaattagc caaaaatcaa
     1081 ctggatttca tatagactac ataattttaa attgctaaaa aagacaatat gttgacaaaa
     1141 atagaatatg ttgataaaat gaaatagtca atatggtgtc ttaatttaaa aatttattta
     1201 tgttgtatta gataaagctg ttttatataa aacataaata attattataa ggagaaatag
     1261 attatgaaag taaatgtata caatgtagaa tcatttgtgg atgaaagact taagattatg
     1321 gtttcggact taattccaaa tactaaactt aaaataagtg tgaaaatgga atttccatgg
     1381 tgcaagggag aagaattttc atcttatgga gtgttttgtt caaatgaaaa aggagaagta
     1441 gatttagatt tagttgaacc tatagaaggt acttataagg ctgataattg tatggggtta
     1501 atttattcgc tggaaaaatc taaaactgaa ggtaaaaatt ttgcagaaaa tatatctatt
     1561 gataaaccaa taattatgaa tatgatattt gaaacttcta ttgaaagaaa agaagttgaa
     1621 ttaaagagaa tatttaaatc agaagatatt accacaaaat atatatctga tgagtttata
     1681 ggaaaattat tttataaaca aaattctaat aataaaacaa ttttaatgct aggtggttct
     1741 gatggaaatt tagacgctct agacctttta gcagctccac ttgcatctag aggattcaat
     1801 gtactaacag ttgcatactt tgctttagaa gggttaccag ataaattaga agaggttcca
     1861 ttagaatatt ttgaaaaagt atttgagtgg attagtgaaa atgaaattac cagtactaaa
     1921 gaaatatttg tacatgggac atcaaagggt ggagaattgg cctttttact tgcttcaaga
     1981 tatgaacaaa ttaaaaaggt tgtagtatca caacctcaca tttattgttt ccaagctctt
     2041 aatggattga tgagtggaaa tgatacttca tcatggtcat ataaaggaga gccatttcca
     2101 tatatcaaag tagacaatga tatattcttt gaagaacaga aaaaaaatat atcaaaaggt
     2161 attccatttg gatttaatag tacttataag aaaagtctta aaagagcaga gaatagaaaa
     2221 gaggctagaa taaaaataga aaattccaaa gcagatatac ttatgattgc tggaaaagaa
     2281 gataacatat ggaatagtta tgaagcttgt ttaaaggcat tagaaatctt tgaagaaaat
     2341 aattatccat ataacgtaga attattaaca tacaataata tgggtcatcc gttaccaatt
     2401 ccatatatta tgcctctaaa ggagacttta tgtatgagta tgagtggagg gattttttct
     2461 tcaggtggaa ctgtagaagg aaattcaaaa ggacagtttg aatcatggaa tagaactatt
     2521 gaattttata aaaaaccttt atcaagtgat ttaaataact actattaagt gatttaagta
     2581 actaaaaatg aagcggtctt tctatatatg cacaaaatct atcaatttta tagatttaat
     2641 ttattgttta tataatttgc atgttatata ctaattttaa tgacattgat aatttataaa
     2701 caattataaa gttgtaaact tataagagtt attgaaaggt ggagtgaagt tatggaaaga
     2761 ctcgctagaa gggaacaacc tcatataaaa tttggaatgt ttaagataag aattccattt
     2821 atacattaca gatttgagaa accagaagct attcaaggta taatatcaag tatgacaagt
     2881 ttaggcacaa ttggccttag tacacaaatt ttgggattac cctatgaagt ggcatggagt
     2941 atggctatca ttaactcaat attatactgt ttgcatgttt ttatgggaga cccagttgta
     3001 cctggctgga ttacagcttc tataactctt acaactgcat atttgttaaa gttcagcatg
     3061 ggcattgaaa gaatacaagc attaacagct ttacaaatag atttaggaat aatatttatt
     3121 ttaatgggta taactggtgt agctggaaag ttagttagta agataccaaa ttctattaaa
     3181 ggtggaatac taatgggtgt cggtatatcg actataatat cagagtttaa tcctaaaggg
     3241 agatttgatt cttatcctat atctataaca gtaggaattt tggttgcttg ttttgtaatg
     3301 ttttctgaaa gatttgatac tctaaaagtt aagaataaat ttttatttca tttaggagaa
     3361 tatggtgtag tatcagctat tttagtgagt ttaatagtag gtatatttag taaagaaata
     3421 gaaattccta gatttagttt tgacaattta gtttacatag tagattttaa aaatctgata
     3481 aatacagtat ctccttttgg aataggtttt ccaagtgtaa tgttatttat acaagggata
     3541 ccaatggctt ttatgatata cataattgcc tttggtgatt ttataacagg tgaaaactta
     3601 gtactttctg aaagtgaaaa tagaaaagat gaatatatag atttcaattc aaataggtct
     3661 aatgttataa gtgggataag aaatattttt atggcaatct tttctccata tataccaatg
     3721 tgcggtccat tagctgcgac acttacagga agtgttgctc aaaggtacaa ggttggaaag
     3781 gaagccatgc agtctgtttt tagtggcatg ggtacattag tatgggtttc agcaattttt
     3841 atctgttttt atccaattgc gcagattgct acacctctta taccattagc attgtcagta
     3901 acacttttag ttcaaggata tttatgtaca aagctatcaa tggagctatg taatacaggt
     3961 gttgagaagg gattgattgg acttatggga ggtgtaattg ctgctaaagg cggaacatgg
     4021 ggacttgctg taggttttat actgtatttc attttaattg attctaaaaa aagaaaagaa
     4081 aaagatatta attcaataga agattgtgta tatgaaatat tagacaaaaa gaagagtatt
     4141 taaaggaaaa tctgagttgc tgttatctat taatagatta attgcaactc atgttttatt
     4201 tataagtttt aggaattgca aaaaaatgca aagctttttc ttgacataat ttcttgatat
     4261 gttactatag ttatataaca catgtgtttt ataaaaattt aatggagagg tgaacatata
     4321 tgccacctaa agtaaagata acaaaagaaa tgattttgaa tacagtcttg gatattacaa
     4381 gagaagtggg atttgaagct gtaaatgcga ggagtattgc aagtaaatta cagtgttcca
     4441 ctcgaccaat ttttacttgt tatgaaaaca tgaatgaatt aaaaaatgaa tttcttgctt
     4501 ttgcatatga atattataat cagtatgttg ttaattatcg taattctaaa agtgtaagcc
     4561 cttatttact gcttccgctt tcatacattg aatttgctca agaagaaacg tatttattta
     4621 agttgttatt tataaatgat ttagatttga tgatgaaaga ggcgaatgat ttttataaag
     4681 aaattgacaa tgaaaaaaag gcaagacttt tttcggaaag cattggaata gatttggaat
     4741 gtgcaaaagt aatattttta gatttatttc tttatactca tggcatagct gtcttaacag
     4801 caacaaaaaa attaacatta gatagatata gtgctgaaaa aatgttgaaa aatatattaa
     4861 cagcttttat aagacaagaa aaatcagatt ggaatttgtc tatttgatgt ttattaacta
     4921 atatagaaga ggtgaaatta tggaaacaaa acaatactta attgactttt ataatactta
     4981 tgatgaagat agtcgacttg cattaaaaca tggaatggtg gaatttctca ctactatgca
     5041 ttacattgat aagtacataa aatcaggtga ttgtgtgctt gaaattggcg ctgcaactgg
     5101 tcgttattct cacacactgg cacgtcaggg atatgatgtt gacgcagtgg aattagtcga
     5161 acataatata gaggttttcc ataaaaatac tcaatcaaat gaaaatataa gcattactca
     5221 gggtaatgct atggatttat ctgttttccc agataataaa tatgatatta cgttactgtt
     5281 aggaccacta tatcatcttt ataacaaaga agataaacaa caagctttac atgaggcaat
     5341 tcgtgtaaca aaacctggtg gtgtggtttt tgctgcatat gttatatctg atggctgtct
     5401 tattgacgag gggttccata gaggaaatat taatgtatct gaatatattg aaaaaggttt
     5461 gattgatcca cagacatttg ctgctaagtc tgaaccaaag gatttgtttg agcttgtacg
     5521 taaagaaaac attgatgatt tgatgtctgc atttaatgta actagactgc attatgtcgc
     5581 atcagatggt ttggctttgt atatgcgtga agcagtagac tctatggatg atgatgcatt
     5641 tgcattatat ttaaaatatc accttgctac ttgtgaacgt gaagatttag taggggttac
     5701 aagtcatgca attgacatat ttagaaaata aggaatatgc ttgtttatgt ctctggtgat
     5761 gttgctcgga gatataagta agtgtatctg tataaaataa aaacaagtat agggatagaa
     5821 aattatgtaa agtcaaaatt ttctatcctt ttagctttta aaaaattatg ttaaaacttg
     5881 tataaaaaat ggctattttt tatatacatt cttactgaga acgtaagcgt tctgtaagtg
     5941 attttttttc tatatttctt acaagtaatt cagtcactaa aacttgtata ccaattaata
     6001 cgaatgaaaa aattaaaata gtaaatatag aaaatttaaa attaataaag tctaatctaa
     6061 ttacatcttt tataaaagtg caaagtagat ttgaaccaaa ataacctagt atacttgaag
     6121 tacatatagc tttgatactt atgtttagac cttctctata aagcatattt cgcagttgct
     6181 ttcttgtcat tccaacggct tgaagtaatg caaactcttt ttttctaatt aaaacgctgt
     6241 ttattagggt attggtcata tttagtatac caaagcaaga aattaaacca acaaaaatgt
     6301 atgaagctat aattatatat tgaaatgctc tagtaatcat tctataatca tcttcatatg
     6361 aataaagcat taaatttgaa tttttctgaa ttatacttaa caacttttct tcaacttctt
     6421 tagaatgctt tttatctatt aaaatagaaa aatctaaatt tgtattatat ggtgttagag
     6481 attctaaagt tttgtctgaa gcaaaaaaca tacctcctgt attggaattg tcaatgattc
     6541 ctataatttt cattttcttt ttaataatat catttccact aaagatatta aatgtaactg
     6601 tatctccaac ttttaaattt aatccataat attttgttct atatttattt ataattaaac
     6661 tatctgaatc taaatgtgta tagtctattt ttccactgac aatatattct tcaagtgatt
     6721 ttgaaagttc aggcgtaata cctttaatta gagtaataaa ttgtttttcg tctctatctt
     6781 tgatgtaagg ttctataata ttacttttaa ttgatttgga ttcaattaat ttttttactc
     6841 catgaataga tttaatttca ttcttaagag aatctgttag tggattattt tgttggattt
     6901 ctgtaatatg ctcatataag ttatcactct taatgctaat tatataatct tcgttataga
     6961 caaattgttg agatagttgt tttttgaaat ctattgaatt agcaagatta gcgattgaaa
     7021 taaataaaac tccactaatg cttagcgata ttaatgcaat caaatttttc tttctatttg
     7081 actcgatatt ctttaatgct agatttttta tatttatata actaaaattt tttgcttttt
     7141 ttctaaattt agtttctgta tattgcatgg cttcaatagc agatatttta gaaataattc
     7201 tagctggttt aaataaagaa attctaacag ttaaaaacat taccagtgat aaaattaaaa
     7261 ttataaataa atcagctatc atttgccatg ctaatggttg aattatgtaa gcaattatac
     7321 aagaagctat aacccctaat ggaataaaaa taattgacaa aatatttcct tgtagagaaa
     7381 taattttttt taactgaaaa gaagtcatac ctaacgattt caacttagca tacatctgaa
     7441 ctgaattaac aactgaaata taaaaaatac tatatataac aaaaacgcta gaaaaaagaa
     7501 caactattcc aactactaaa actgtaataa tctgctcacc atcaattaaa tttgattcaa
     7561 tatagttatc atttaagtat atattatatt tcttaatacc taaagataaa ccaattttat
     7621 ttaactgtgt cttaattgaa gctgaatctt tctctgtatt ttcttctgat agtttaacta
     7681 atacatctac agtatcaggt cttgactcgt caaaagtatt acttttattt tgtaaaggtg
     7741 tagaagctat attcttaaaa taatcatttg aaacgactgt atagtattgc ttggcattat
     7801 cttgataata attttctaga aatccaacaa taataaaatc acattgaact tctttattcg
     7861 taatatcatc ataataattt aatttaatgc tatctccaat atctttatct aaaccaaatt
     7921 cctgtaaata tttttctgaa atagcaattt catttttatt tgtaggtagt ttgcctttca
     7981 atggttgaaa attcataagt ttaattgctg tatcatctgc ataaacaatc gacataatac
     8041 aatctgtctt tttattactt ccaattaatt tgtaagttcc tacgccacta aagtttttat
     8101 ctttatatag tatatttttt gctttttcat ctacagattt atatatagca tgatatggag
     8161 atgcattaac taaatctaca gaggcattat atgaaactaa ggtcactgtt aataataaac
     8221 atgtagaaat cattataatg atacctgaaa gatatgtttt aagtttatta atttttaaat
     8281 catttaaagc tatttttctt ataaatgtgc gattattatt tctaaacata tactacacct
     8341 cctagttaac aatttttcca tcttcaatac gaataattct atcagttagt tgtgcaattt
     8401 cattattgtg tgtaatcatt acaattgttt ggttgaattt tttacttgtc actttaagta
     8461 aactcagtac atctgaacta gttctactat caaggttacc tgttggttca tcagctagta
     8521 taatagctgg ttttgttaca agtgctctag ctatagcaac acgttgctgt tgaccaccag
     8581 aaagattact tggtatatca tataattttt cttcaagaga aagcatattt ataatttcat
     8641 ttataaaatc cttatctatg gtctctccat ccaatcctat tggtaagata atattttcat
     8701 atacattaag taaaggaaca agattataat tttgaaatat aaaacctata tttctgcgtc
     8761 taaagattgt aagctgttca tcagacatag tagataattc cttacccttt ataataacct
     8821 ttccagatgt aggattatct aatcctccca tcatatttaa taatgtggat ttgccactac
     8881 cagatgtacc tacaatagaa ataaattcgc ctttttcaat agatagagtt acaccatcaa
     8941 gagcttttgt aatgtttgaa ctttgtccat aatatttctt taaattaatt acttgtaaaa
     9001 tactcataat aatacctcct taacatcttg taaatacagt ataaaaccaa aatgttgcag
     9061 aatcattaca aatgaaatat ttaaaaattt atttattagg taagaaaata gaaaaagtcg
     9121 acccttttcc aatatctgat gtaactttaa tatagccacc ttgaagagat ataatttttc
     9181 ttgaaagata gagacctatt ccaatacctt ctatgtcatg gacatcttct tcacgataaa
     9241 atcttttaaa gatttctgct tggcgacttt ctgatattcc ttttccatta tcaataatat
     9301 ctatttttgt atacataacc caacattctg tagctacacg tatcttacca ttagaacttg
     9361 tgtattttac agcattatct aatatattaa aaatagcttc agaagtccat ttcttgtcat
     9421 gaattatata taaatttggg tcacaatcaa ctgtaacttc aatattcttt ttttcagcat
     9481 caaacaaaat tccactaagt gctgtagcaa ttgtttcata aattgaattt ttctttttag
     9541 atagtgtgat tatacctgtc tctagtctgg aggtttttac catagattgc attaaaaaat
     9601 ctaatttatc taattggttt tccatatcca ttaaaaaatc cttttgaatt tctgttttaa
     9661 atgtttgatt tagtagagta gtattaatca tttttaaatt tgcaatagga gttttaattt
     9721 gatgagatat atcagaaact agttcttgta aatcagcttt ctctatagca atactattgc
     9781 gattttcttg aataacttca tacattctaa ttaatctgtg attgatacgt gataaaatag
     9841 tttcttcttc taattcaatt tcaatattat cttcactatt catcatacta tctaaagtgt
     9901 gacatattct agaacaaaat ataactattt ttttatgaag taaaaatatt attaatgcta
     9961 taaatccaca aagtactatt gtatatatta ataatagagc aattagtttt aaatcatcag
    10021 ttattagata aattgagata ctaaataaaa tagctgtaaa aattatacca acagcaatta
    10081 ttaaataaat agaactaaaa gaaaattttt taaaattcat ttctttcacc taaccacata
    10141 tatcccatac catataccgt tttgatatac ttaagattgt ctttttcaat tttgcttcta
    10201 atcctactta taacagatgt taaagtatgt tcatctacaa agttagcatc tatatcccag
    10261 agtttttcta aaagtacttt ccttgttaaa atacttttag gattttcagt aagcagtttt
    10321 aaagtacgat attctaatgg tgtaaatata atttgatttc catttatatt ggcagataac
    10381 tcagaaaagt taatttcaag atatccatcc ctatagtaat cttgttttgt ttttattgta
    10441 agatgattta aaaaagcttt aattttttgt ttaaatatat tgatattaaa tggttttgtt
    10501 atataatcca aagctccaag ttcatagcct ttaatcatat cacattccat atcattagca
    10561 gttaaaaaaa ttattggtat attgtaattt tcctttattt cattacataa ttcaaaccca
    10621 cttttatctg gtaaattaat atccaaaatt attaaatcaa attcacattc aaaaatagac
    10681 tttagtgcag atttagctgt aaattttgaa gtaattatat atccatcttc aattaggtta
    10741 tatgatagag ttttatttaa tatagaatca tcttctacta ctaatatttt ttccatacag
    10801 ttactccttt tagtttaata ttttataatg tcaattatag actaaaaaca ttgctgaatt
    10861 gttataaacg agaaaaagta tttaaaaatt tatatattta ataaagaacc tgtcctatga
    10921 taaattctag tacagattct ttattaaata aaattatttc aaccaagatg gatttccagg
    10981 tataaatatt cttttaccaa attttgaagt atactcatct aaagcttttt gaataagctc
    11041 attattaccc ttaaatgaag tagtattctt tatagtttcg agattttggc taaatgggca
    11101 agcagatata cacataccac aatctgtacc taaatatctc catttaatat agcatgtctc
    11161 atgttcaatt acccaattat attttccatc atcaccaatt ttaatatcat tagaaagcga
    11221 atgagaaggg cagttaaaag agcatttttt acagacctta caaaaatctt ctagtccaaa
    11281 atcaacatat tcatcttcta aaagaggaat atctgtagta acaacaccta atctaagtct
    11341 agagccataa tctttatttg taagtatagc atttcttcct atatctccta aaccagcatc
    11401 acgagctata aaaacaggca ttactaaata attagcatcc atatgatttc tagcatcata
    11461 tcctaaattt cttatatagt aacttaaaat catgccgacg atagatgcat ccacatagca
    11521 ctttgatgtt tctataactt cacatatcat tggagctcta tttatcattt ctttatccat
    11581 ctcacatcca aatacaattg catacttatg agttagatta atttcttcac cgtaattttc
    11641 ttcatgtcta cccctatttg tataaatatg atagtctttt aatttagtta taccaactac
    11701 agaagctcca tactgctttg caagaccctt aatttttttt gtcattgact ttgcatctac
    11761 atctacttta gtatcagaaa cttttccttc gcataaagat ttgatatcag ataaaaaatc
    11821 gaatgcacta cttgccattg gagagtttaa ctcattgtaa gtcattgttc cttcactaca
    11881 taaatttggt ctatttctta tactatcatc aatttctttt ttgtcaggat ttttcttata
    11941 atagtcatta taagcagtac tatctttttt gtaatttgct cttgcaaaca tagtatttct
    12001 ctcatcaacc cttttcaatt ctattccccc tagtaaaaat atatttaaaa aatataactt
    12061 tattgttgta taatttaatt atataagaat aaagttatat tttcaattta agcgt
//
LOCUS       DAYASV010000115        11928 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYASV010000115 DAYASV010000000
VERSION     DAYASV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.116"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(202..1932)
                     /gene="lpdA"
                     /locus_tag="ACGI68_002933"
     CDS             complement(202..1932)
                     /gene="lpdA"
                     /locus_tag="ACGI68_002933"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFH0139360.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            complement(2099..3145)
                     /locus_tag="ACGI68_002934"
     CDS             complement(2099..3145)
                     /locus_tag="ACGI68_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFH0139361.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTAKETIVSTNTSKSSE
                     KKELKIENENSSMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            complement(3158..4144)
                     /locus_tag="ACGI68_002935"
     CDS             complement(3158..4144)
                     /locus_tag="ACGI68_002935"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFH0139362.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            complement(4169..5131)
                     /locus_tag="ACGI68_002936"
     CDS             complement(4169..5131)
                     /locus_tag="ACGI68_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HFH0139363.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            complement(5169..6122)
                     /locus_tag="ACGI68_002937"
     CDS             complement(5169..6122)
                     /locus_tag="ACGI68_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFH0139364.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            complement(6567..7940)
                     /locus_tag="ACGI68_002938"
     CDS             complement(6567..7940)
                     /locus_tag="ACGI68_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139365.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            complement(8037..9683)
                     /locus_tag="ACGI68_002939"
     CDS             complement(8037..9683)
                     /locus_tag="ACGI68_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFH0139366.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYVQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            complement(9705..10871)
                     /locus_tag="ACGI68_002940"
     CDS             complement(9705..10871)
                     /locus_tag="ACGI68_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HFH0139367.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFAMIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYKVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLVKCVAQEWYPIEY"
     gene            11088..>11928
                     /locus_tag="ACGI68_002941"
     CDS             11088..>11928
                     /locus_tag="ACGI68_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139368.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNL"
BASE COUNT         3643 a         2312 c         1279 g         4694 t
ORIGIN      
        1 taatgttatt ttatattact atcttttttg tacaacaaaa actacaagga ttctatattc
       61 tatatttttc cataaaaaat accccttatt atgcaaataa attatttgca taataagggg
      121 tatggctaag taagtgtttt aaaattttat gatataaatt aatattaata atagacttaa
      181 attaataatt tatgtttaga tttatttttt aggtaagtgt aaacattctc catttacatc
      241 tgccaaagct tccattaaag cttctgaagt acatggatga ccaaatatca actcacttgc
      301 ttcatcagta ggtatctcta atgctttaaa tgatgctgca tgatttataa gttctgctac
      361 accacatcca aacatatgta tacctaaaat ttctccatat ttagcatctg ctactacctt
      421 cacatatcct tgttcttgac cagaagctaa agctcttcca tttccagcaa aattaaactt
      481 accaactttt acattatatt tttttctagc atcctcttct gtaataccaa ctgatgctac
      541 ttctggaatt gtataaacac aactaggtaa tgcacctaag tcaacttctt tattcacacc
      601 tagagcattt gaagctgcta cctctcccat tttaaaagca gcatgagcta acattacacc
      661 tccagtaaca tcacctactg catatatgct tggtatactt gtttccattt tactatttac
      721 aactattgaa cctttatcta ttttaatatc taagtcctct atgccagaaa gattagcttc
      781 tcttcctata gcatatagac ataaatctgc ttttattggc tcttcacctt caatgcacac
      841 taatatgtta tttccttctt ctttaaattc agaaactttc tttttagtta atacatttat
      901 acctttttta ctaagagaat attttaaaga ttcgcttaac tctttatcca ttcttgggat
      961 tactctatct tccatttcta ctatagttac tttagaacct cttgaattga atatctctgc
     1021 aaattcacaa ccaatcactc caccaccaat tattactaat tcttctggca ctgtttccaa
     1081 atctaatgct tctgtactag ttattattaa atttgattct attcctttta taggtaatat
     1141 ccttactttg gaacctgtag caattattat attttctgta tctaaaactt ttccatctga
     1201 taaaattact ttatgctctt ctttaacact agctttaaga ttaaacacat ctacatctct
     1261 actttttaat aatccgccaa caccagctgt aagtttttta actactctat ttttatattt
     1321 tatggccttc ttaatgtcct gttctttatc tacagttact tttacacctc tctttgataa
     1381 ttggtcaatt tcttctaata tttctgctgt ttttatataa gtctttgtag ggatacaacc
     1441 tctatttaag caagtacctc ctagtatatt ttcttcaact aaagcaactc ttccacctaa
     1501 aagagctgct tttaatgctg ataaatatcc cccaggtcct cctcctatta ccactacatc
     1561 ataatcatga ttacactctt ttttgtttat tttttcttct ttaaattcta tattttgatt
     1621 ctcttcaact atatcttctt ttgaaacatt tccagataaa tatttagctt ttacttcctc
     1681 ttgattttcc cctttttctg ctattacacc tattactgta aatactggta atacttcacc
     1741 ttcctcttta tgtatgatta cagcaagtgt accttctcct tctgactcta tctccatatt
     1801 aactttatct gttgttattt ctagtatagg ttctccaatc tttacttctt cgccttcttg
     1861 ctttagccaa gatactatag taccttcttc catggctact cctgcttttg gcattattac
     1921 ttctactgac ataggctctc ctcctattta ttttcttata ttttaattaa gcaaatcata
     1981 ttcacatata gttactgtta aaaataattc ttaatatata aaagctttaa atttactgaa
     2041 acaattataa taatataatc tattcaaact atttatatca gtaacaaaat atcaaaacct
     2101 atacaagcat tgataaagga ttttctaaca attcttttaa atcttttaag aattttgcag
     2161 ctactgttcc atctattact ctatggtctg atgttaatga taagttcatt ataggtttta
     2221 ttttagcctc tccatttact ggaacaaact tatcttgtgt agcacctacc cctaagattg
     2281 cacttgaagg catatttatt ataggtgtaa atgtagttat accatacatt ccaacattac
     2341 ttattgtaaa tgtattacct tcttggtctg ctggcataag tttccctgtc tttacttttt
     2401 ccgcaagttc tttgctttct tttgctattt cttttaaaga ctttttgttt gcatttttta
     2461 ctaccggaac atataagcct tcatctaaac caactgcaat agctatattt acatctttat
     2521 atctaaatat accctcatct gtccatgctg aatttaaggc ttgatgattt ggaagtattt
     2581 tagatactgc cattactatt aaatctgtca tagttaattt tactccagtt gattctttta
     2641 ctgtgtcgat taactttgct ctaagtacct tcaattctgt agcatctact tctatattga
     2701 atgtaaatac aggagcacta aaataacttt ctgacattcg ttttgctaca gtagctctca
     2761 ttggatttgg cttttcaaat attccttcta ctgtgttaaa catgcttgaa ttttcatttt
     2821 caattttcag ctcttttttc tcactagatt tagatgtatt agtcgataca atagtttctt
     2881 ttgcagttaa tttttccaca tcacactttc ttatctttcc attatatcca gtacctacaa
     2941 tagtttctaa atctatattt aaatcttctg ctattcttct agctaaagga gtagtatttg
     3001 ctttattatc ttttaaataa tttaacacat ctactaaatg aatccttcca ttttctccac
     3061 ttccaatcag cctatctaat tttatattgt cttttcttgc ctgacttcta gctgctggtg
     3121 tggcttttat tttatttgcc accatatcta tccctcctta tctaacaatt aaagatttta
     3181 ttgcttcttc tatttcatca actcttggga caactgcttt ttctaactct ggattatatg
     3241 gtattggcac atcttttcca caaattcttt ttactggagc atctaaataa tcaaatgatt
     3301 cactttctgt tatcagtgct gatatttctc cacctagccc accagtctta gctgcttcat
     3361 ggcaaattaa aactctccca gttttacata cactctttac tatagtctct ttatctaatg
     3421 gatataaagt tcttaaatct ataatttcaa catttatatt ttcttttgat agagtctctg
     3481 ctgcttcttc aacactttgt aacattcttc cataagtaat tactgtaaca tcagtaccct
     3541 ctcttttaat atctgccttt ccaatttcaa ttacatagtc atcttctggc acaaaacctt
     3601 tttttctgta taacaattta ttctcaacaa atatgactgg attattatct cttatagctg
     3661 ctttcaacaa tcccttagca tctgctggtg tagaaggtgc aactactttt actcctggta
     3721 catgacaaaa ccaagcttct aaactttgtg aatgttgctc tgctgaacca gttccagaac
     3781 ctcctgggca tctaacaacc attggaactt gtgcttttcc tccaaacata tatctcattt
     3841 ttgccgcttg atttacaatt gcatccatag aaattgttac aaagtccata aacattactt
     3901 ccataatagg tcttaagcca gttgctgctg cacctgctgc cgctcctgct atagctgctt
     3961 ctgatatagg agtatccctt actctttctg gtccaaattc atctatcata ccaactgata
     4021 caccaaatgc tcctccatat attcctatat cttctcccat aaatattaca ttttcatctc
     4081 ttctcatttc ttctgacatt gcttccttta ttgcttgtgc atatgttaat tcccttgtac
     4141 tcatttttat tttcctcctt taccattctt agtctgcata tacatcctct aataaagact
     4201 ctatttttgg atttgggcta ttttgagcaa actctactgc atcttctatt gattgctttg
     4261 caaattcttg aatcttatct aattcatctt cattagatag attattttct ataagataat
     4321 tttttaaaaa ttcaataggg tcttttgctt tccatgattc tatttcttct tttgttctat
     4381 atacatttgc atctgatttt gaatgaccta accatctata agttctactt tcaattaata
     4441 caggtccttc tcctcttcta cacttctcag cagctttttg tactgtttcg tatacttcta
     4501 ttgggttata tccatctatt gatattcctt ctattccata agaagctgct ctagtggcaa
     4561 tactttctat attcatatga cgttttatgc ttgttgacat accgtataaa ttattctcac
     4621 agtaaaatat tattggcaat ttccatattg aagataaatt tattccttca tgaaaagtac
     4681 cttcatttga tgctccatca ccaaaactac ataaaactat tttgcctgtt tttttatact
     4741 gttgtgtaag tgcagctcct ggagctattg taagacctcc acctacaact ccatttgctc
     4801 ctaagttacc tgactcaata tctgctatat gcattgagcc tccttttccc ttacaaaatc
     4861 cagtttcttt acccataagt tcagccatca ttttatttag gtctattccc attcctataa
     4921 attgactatg tcctctatga gttaatgata ctaaatctcc tttttctaaa gccattacag
     4981 ccgccacact tgacgcttct tgacctacag ataagtgagt tgttccatga accatacctc
     5041 ttgcaaaaaa ccaactaact ttctcttcaa attttctagc ctgattcatt ctcttataca
     5101 tttcaagcaa tgtttctttt gatattgaca tatacattcc ctcctgttaa cttttatcta
     5161 tctttctatt agtcacaatc ttctaataat ttaagtgcta aactgtgatt aattgctaaa
     5221 ctatttataa atttcgcttt taatgatgcc tttattgcct ctattttttc atctcctgaa
     5281 gcgacaccta ttgttctttt tatctttttt aaatcttcca gtttcactcc taatactctt
     5341 tgattaaatt caagtataga agtttttcct tctttatcaa aggcttgtaa acaaattgca
     5401 cctattgagt tgtatttttt taaatcttct atatctgctt cattcatgta tccagaagcc
     5461 attattgttg agtttaaact cataggattt cctataccca caattgctat agttgtttta
     5521 tctattaaat ctagtacttc tttaattttt tcgtctttgc aaagtttctc ttttgtagac
     5581 aagtcagaca taacagctgg agcatgtaat aatttaaaat cacctccaaa agccctcgct
     5641 aaacttacgg ctatttgatt tgcatgaata tctatttcat tgtctccaat ccctcctaat
     5701 agaggtataa atgtaacatt tttacagtta cttttttcca catatcttgg tatttctttt
     5761 agtgttgttc ctattgaaac acctattata tcattttcct ttactactct agttaaatat
     5821 tcagatacag ctcttgctag ttcttgtttt tgtgttagtg catcctgttt atcatctact
     5881 actattactt cttttaattt atattttctc tccaaacttc tctctatttt ttggtaatca
     5941 tttttcaaaa catcaactat ctgtattttt actattcctt gttcaaaagc ttcttttaat
     6001 attcttgata ttgttggtct tgatattccc aattgatttg ctatttcctg ttgattcaaa
     6061 ttatcttcgt aataaagtga acaacacttc atcattaatc ttaagtctcc aatttttttc
     6121 atttgctttc ctccaaaatc aagcttttca attatcttta catttgttca atcattttac
     6181 ttaatttcag taacattata gaaggggttc atataaaaag tcaactgttt ttttgtataa
     6241 caaaaaaaag gtgatacttt tcttgtacca ccaccttcat aaatagttga aaaattgctt
     6301 cttagtgcat attatatatt acttctaatt gttaaatatc atctccataa attaaaaatt
     6361 aattgtccac ttataatgct ttaaataaca ataacttatt aaatcacttc taaaaaacca
     6421 accatttcat catttaaagt cttcttatat ttttattatt cgcttctcta aaaattcttc
     6481 aataatcata aatatataat agtttttata gggaacattt acaatagacc ttaattaaac
     6541 ttaagacttt ataagaaact ttttcattat ttagtattta attcattaag tatagtacta
     6601 tattctttat caagcttata aaaagacatt atgataattt gtaatattgt tataaatata
     6661 ggtaggtata taaaacatac ttttattgat actatagcta aatcactttg agtagctgat
     6721 gttcctacat aaccaccaat tgataatact gcacctaata ttgctgaacc taatccattt
     6781 cccactttaa acccaaaact agatgcacta tttataagcc cctcagttct atatccagtc
     6841 ttccattctc catattcaat agtatctgaa acaatagcaa acattgttga tgctattcca
     6901 gcacttccta ttccctttat agcacttgca aatattactc ctatatgatt ttgtccaact
     6961 attataaaca taacatttga cgatattaat attattgaac ctactattaa tacatttctt
     7021 tttccaaatt tttttataaa aggagcaacc ataaacatag ctataatttg ggatatattt
     7081 gaaaccatat ttataggacc aaataatttt tcatctccca aaataaactt tgcataatat
     7141 acggatgaac caccatttat cgcaaataca ataaatatgc aaactaaaca taatgttatt
     7201 tgtatccagt acttatttct aaataaagat ttaataccta ttttaaatgg tattacttca
     7261 ttcctatctt gcttaactgc ttttactctt tccttggttc cagtaaatgt tataaaaaat
     7321 accattatag atgctattcc aaatacaaag aatgttttag tccatgctga taaattattt
     7381 ccaaagtatt caactagagg tagtgtaagt ccatttatta gtaaggtacc acaagtggct
     7441 aatatcattc taaatatact taatactcct ctttcatact ggttttgagt tataagtgaa
     7501 tttagagttg catagggtac attgattgct gtataaataa cagtagaaac taagttatat
     7561 gtaataaaaa tataaattaa tttagaagtg ataccaagat ttgatggaac agaaaataat
     7621 aatactgctg ctatagcaaa aggaatcgcc attctcaaaa tccaaggcct agccttaccg
     7681 tatttggatt tagttctatc cactataatt cccattgtta agtcactaaa cccatccagt
     7741 atcctagata caagcataat actacctaca gctgctgcac taacatctgc ataattagta
     7801 taataaaaca gcaaaaatgc tgacatagct gagtatatga tattgcaccc caaatcccca
     7861 caaccataag aaattctttc aaaaaatgaa atcttttcat atctaatcgt agttgcttta
     7921 acattaactt ctgtattcat aataccatac tcctttttgt aagatttgcc ttctaatgtt
     7981 tcttatttca gagcataaga tttacctttg ctctgaaaat tttagtaatg attttattaa
     8041 tcgacatctt ttgattcatc tactatatat tcaaaaaaat caaaatcagc atagtgttta
     8101 tgctttacac gatctgcaca agttaatcct ataaacgtac ctgtaaattc tccatattta
     8161 cagtactcat ctgaaaactt agttgtatca aatacctttc ctattctctg gtagtttttt
     8221 tcatcataac tccattcaaa atatgactta cgaccttgta catacagacg aagatatatt
     8281 ggtatatctt ttattggtat cctagtattt aaaaattccg ttttctctcc attttcaaga
     8341 tgtattattg atagtgcact ttgacccaat gtttcactat aatactttct taaatttata
     8401 taattcatat tatcataata cattatcaaa cccgcactat gctgatgtac ctctgggtaa
     8461 aactccatct tagtggttat tcttgcatat actgaagtta attttcttgc caatatgctc
     8521 actttattta atgatgttct tgattcttgt cctctaatcc ttacatatcc tggtctagac
     8581 ttaacatctg caaatgataa tggtgaaatc ctaggagcat agtactgtaa acctaattca
     8641 tttgaatcaa agtcatcaaa acttggtata gatgaaacta aatgttcttc tagtttactt
     8701 tctgatacgt attcttttgc caaattactt tcatcttcca ttcgaagcca attatccttt
     8761 gtccatttca ttttttgaat agctgtttct ctacctaaag tacaccttaa ctctggtaca
     8821 aatggtctag atgttagatg aactaaatat acttcacctg atgttgtttc tatatagctt
     8881 ccatgaccag atttttgaag ttttgattct ggattatagt atttaggctt tagatggtca
     8941 gggtcatgtc tttcataaaa atcgcctgga attgaagtta caattggatt cattgaatct
     9001 ttttcataag gtccccatat atttttagcc cttcccatag ttacactatg accatatcca
     9061 gttccacctt ctgcacacat tatataatag taatctccac gctttgttat atgtggtgct
     9121 tcaatacaac ctctatcagt tccccctgac catattctct ttggatatcc tactatttct
     9181 ttcttttttg tacagtattc cactaaacat ataacaccag gtttttcata tccttctctt
     9241 gtctcccagt ctaatgatgc aatccacttt ttaccatcat catcatgaaa aatagacgca
     9301 tcaaaacctg atgagtgtag atatactggt tcactccact ctccctttat atcttttgat
     9361 gtaattaaat aattatcaac atcaaaatat cttgcattca tagagttcat aattccataa
     9421 actatataaa ataaatcttc ttcttcacaa taagtcaaac agggtgccca aattccttta
     9481 gatgaaggaa gcttcttcaa atctattttt gtttcatctg tcaaaatatg agtatataac
     9541 tcccaatttt ttaagtcttt agagtgataa acaggtatac ctggaaacca ttcaaaagat
     9601 gaaactacaa gataataatc atctcccttt ctacaaatac aaggatctgg attaaaacct
     9661 ggaagtatag ggtttttaat catatctttt cctccaattt tagtttaata ttctatagga
     9721 taccattctt gagctacaca tttgactagg tcccaactat cccatcctat ccaaccttct
     9781 tcattaactg tatctagtaa taacttataa ttccaatagt agtgaccact tccatttctc
     9841 catgcatcaa gctgtgcttt tgctatcttc ctatataatt ctcttttatc gtcttttgat
     9901 aatttatcta tatttgattc aatcccattt agaggtgatt gacctccatt tgtatctata
     9961 ccacaagcat atgaattaaa taagctccac tccccacata taacaggaaa atatttttgc
    10021 atttgtaaaa tatctttagc ataattttcc ctaatatatt tgagatagct atcaatactt
    10081 tgttcacatc catctgcttc agctaacatc aaatattggt gtgtatctag aacaacattt
    10141 ttaaattctt cctctctcat aaagtctttc catgctttta attcaaatgc atcatgaaat
    10201 actataacgt tttcctccct catataagga cgcattacct tatatgcttt tatataaaaa
    10261 tcacgtaaaa actctaatga attaggttta ctcccccttg ccatctcctt atctacagcc
    10321 ttaaaccgat ttggaacatt cattatgttc cacattcttt ctgttatagg ctcatttaaa
    10381 atttggattc catacaattc atgcctcatc ccataacgtt ttgcaagtct ttctaataca
    10441 tttaatgtaa acgctacata ttctggattt tgtgaccagc tacatatacc tgatattcct
    10501 ccattatcaa atccattttg gcttcctgga actgtatgta agtctatcaa tatactaagc
    10561 ccatacttat ctgcccaagc aaatgcttta tcaagttctt taacacatcc tatgaatggt
    10621 tcacaatctc caaaaataaa gtatggtact ggtatacgaa ctgaattaaa tcccatagat
    10681 ttaatcattg caaaatctct ctctgtgatg tattcactac gatgagtctt aattctagat
    10741 tcatatgcct ctcttgaaag ctgtcttggt aaatagtatt catcttcagc ttctgtgcct
    10801 tcaaataaac ttgaggtcat ccatttttct aaaactaacc aacctcctaa atttacacct
    10861 tttatataca cataaaatcc tcccctctta tcttatttaa ataggtatat tttgcactta
    10921 taataaaata atttttttaa gttattttaa tatacagtta ttatatttga ttttatccgt
    10981 tttcttaagt atgtttatta tctaatttat taatctatat tatcttttta atattgaaca
    11041 ttataaattt actatttata atttaaaatt ataaggagga tattattatg aacagaaata
    11101 atttttcaca tgaaataatt gaacctattg ataatttaga tgttaatttc aaactatttg
    11161 atgatagtgg tagttatgta gcaaatcatt ggcataactc attagaaata atttatataa
    11221 cttctggtga tttacaaatt aacatggagg gttacactta taatttaaaa gctaatgagt
    11281 gtatgttcat taattctgga attattcact caactagatg tatttatcaa aatacctcta
    11341 tattattgca agtaccattg tcttttctaa acaaatatat ccctgatttt aaaaattgct
    11401 attttgactt taaggttaat gccaatgaca ataactataa aaaaaattca agtaaagtta
    11461 aacttatatt agaaaacatg agagaaatta aacttagttc tcctcctgca gctaatctgc
    11521 tctttacaag cttattattt gaacttttat ttgaattata tactaacttt aaaatatctg
    11581 taggtaataa aaatatgaaa aaaacagtac ttgatttgtc aaaatttgaa ccagttttag
    11641 aatatacaaa gattaattac aaaatgccta tttctattaa caaaatcgct aaggtagcac
    11701 acttacaacc agaatatttt tgtagaaaat ttaaacagta tatgggacaa acttacttag
    11761 agtatttaaa tgatgtgcgt atttctcata tttataaaga cttaattaat acaaatgata
    11821 ccttatgcag tatattagaa gctcatggat ttactaatac aaaattattt tacagaattt
    11881 ttaaggaaaa atttaaatgc actcctaaac atataagaaa aaatctac
//
LOCUS       DAYASV010000116        11722 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYASV010000116 DAYASV010000000
VERSION     DAYASV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.118"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            53..178
                     /locus_tag="ACGI68_002942"
                     /pseudo
     CDS             53..178
                     /locus_tag="ACGI68_002942"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
     gene            233..499
                     /locus_tag="ACGI68_002943"
     CDS             233..499
                     /locus_tag="ACGI68_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFH0139369.1"
                     /translation="MAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDYLIKVL
                     AEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            548..1132
                     /locus_tag="ACGI68_002944"
     CDS             548..1132
                     /locus_tag="ACGI68_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HFH0139370.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1137..1460)
                     /locus_tag="ACGI68_002945"
     CDS             complement(1137..1460)
                     /locus_tag="ACGI68_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0139371.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1612..2130
                     /locus_tag="ACGI68_002946"
     CDS             1612..2130
                     /locus_tag="ACGI68_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0139372.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2306..2974)
                     /locus_tag="ACGI68_002947"
     CDS             complement(2306..2974)
                     /locus_tag="ACGI68_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFH0139373.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3089..4504
                     /locus_tag="ACGI68_002948"
     CDS             3089..4504
                     /locus_tag="ACGI68_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFH0139374.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4703..5185
                     /locus_tag="ACGI68_002949"
     CDS             4703..5185
                     /locus_tag="ACGI68_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139375.1"
                     /translation="MKTNTDVIMEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFT
                     NVVGCTIHQYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQ
                     VYRNKDEFYPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA
                     "
     gene            5182..5766
                     /gene="clpP"
                     /locus_tag="ACGI68_002950"
     CDS             5182..5766
                     /gene="clpP"
                     /locus_tag="ACGI68_002950"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFH0139376.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6078..7154
                     /locus_tag="ACGI68_002951"
     CDS             6078..7154
                     /locus_tag="ACGI68_002951"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0139377.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTDGGFKSDDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7221..9296
                     /gene="bclA3"
                     /locus_tag="ACGI68_002952"
     CDS             7221..9296
                     /gene="bclA3"
                     /locus_tag="ACGI68_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HFH0139378.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAVGATGIGIT
                     GPTGPTGATGADGATGVTGPTGATGATGADGVTGPTGATGATGFGVTGPTGPTGATGI
                     GITGATGLIGPTGATGATGATGPTGVTGATGAAGLIGPTGATGATGADGATGATGATG
                     ATGPTGADGLVGPTGATGATGADGLVGPTGPTGATGVGITGATGATGATGPTGADGLV
                     GPTGATGATGADGVAGPTGATGATGNTGADGATGPTGATGPTGADGLVGPTGATGATG
                     LAGATGATGPIGATGPTGADGATGATGATGPTGADGLVGPTGATGATGATGATGPTGA
                     TGATGATGPTGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDL
                     TNAAGTLTNFAFSMPRAGTITSISAYFSTTAALSLVGSTVTITATLYQSTAPNNSFTA
                     VPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVAG
                     YASAGIAIN"
     gene            9465..10226
                     /locus_tag="ACGI68_002953"
     CDS             9465..10226
                     /locus_tag="ACGI68_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HFH0139379.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKMGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            10535..10813
                     /locus_tag="ACGI68_002954"
     CDS             10535..10813
                     /locus_tag="ACGI68_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3324 family protein"
                     /protein_id="HFH0139380.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            11106..11681
                     /locus_tag="ACGI68_002955"
     CDS             11106..11681
                     /locus_tag="ACGI68_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HFH0139381.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
BASE COUNT         4446 a         1494 c         2195 g         3587 t
ORIGIN      
        1 tattagctaa aattgtatta aataattaag gaggtgagta gctctggatg aaatagattt
       61 aaaaataatt aattctctaa aaaagaatag tagggcttca acctctgaaa taagcagaca
      121 aattaattta tcagtaccag cagtctcaga gcgtataagg aaaattgagc agtcaaattt
      181 aattcaaaag tacactattc aaattaatcg ggaaaaaaca aaatataagc ttttggcttt
      241 tatatttatc aatactgatt tatctaaaag tatgaaagaa tttaagaaaa ctatattgga
      301 atatgatact gtattagaat gtcatcatat ttcaggagag tatgattact taataaaagt
      361 attagcagaa gatacaagta atcttgaaga gtttatatca gtgactttga aggaaattgg
      421 ggggattcaa aaagctaata caaccattgt tttatctaca attaaagaag aatttaatac
      481 aataaaagat attttttaaa taatttacta gtaaagtgtt aattatattt taggaggaaa
      541 atagaatatg aaagttttag tttttttagc aaaaggtttt gaaactatgg aatttagtgt
      601 atttgtagat gttatgggat gggctcgtaa cgattatggg catgatattg atgtagtgac
      661 ttgtggattt aaaaaacaag ttatgagcac atttaatata caagtattag ttgataagac
      721 gatagaagaa gtatgtgttg atgattatga tgctctagct attcctgggg gatttgaaga
      781 atttggattt tatgatgaag catatgattc atctttttta aatctaataa gggagtttaa
      841 ttcaaaagaa aaaataatcg cttctatttg tgtggcagca ttgccagttg gaaaaagtgg
      901 tgttttaaaa aatcgtaaag ctactacata tcatttgaaa aatggtaaaa gacaaagaca
      961 gttaagtgag tttgatgtaa atgtagttaa tgaaccaatt gttgtagata aaaatattat
     1021 aacctcatat tgtccagaaa cagctccaca tgtagctttt aagcttttag aaatgttgac
     1081 atctaaagaa caaatggatg aagttaaatt agcaatggga tttaaactgt aatggtttat
     1141 agattttcta agtaatcaga accccagtca cacatggaat ctaaaattgg aataatactc
     1201 tttccaaaat cagatagcga gtattctact tttggaggaa caacaggata aaccactctt
     1261 ataattaaac catctgattc taattcacgt aattgttgag ttaacatctt aggcgttgct
     1321 ttaggaatta atttttttaa ttcattgaac ctcaatatag tgtctttaag atgccaaagt
     1381 ataagtgtct tatatttacc accaatcaat gcaagtgttg cttctattgg gcaactgtaa
     1441 ttatctaaac attttttcat ttttgggctc ctttatttat tttaaagtat ctttttaggt
     1501 actatattac aaaaaagtgc attattgtaa tatagtacca tagtgataaa attatagcat
     1561 aggttaaata aatcatcaac ttagcctaat aaaacaatag gaggtaaata aatgaatttc
     1621 gttgaacttg caaaaaaaag atattcttgc cgtaattatc aagatagaaa ggttgaaaaa
     1681 gaaaaacttg agaaggtttt agatgttgca agaatagctc caactggtgg taatcgtcaa
     1741 cctcaaagat tgattgttat tcaggaaaaa gaaggtataa ataaactttc taaggctgcc
     1801 aatatatatg atgctccatt agcaattctt gtatgtggag ataaagacaa agtttggaca
     1861 agaccttttg atgggaaaca acttacagat attgacacaa gtatagttac agaccatatg
     1921 atgttacagg caactgaact tggattggca agtgtttggg tgtgctattt taatccagat
     1981 ataattagag aagagtttag tttgccagat aatctagaac caataaatat attattgatg
     2041 ggatatgaat ctaagatacc agagagccca gagagacatg aaaaaactcg tgttccttta
     2101 agtgaaattg tttcttatga aactctttaa ttataaaatt aagttattca taggataaat
     2161 gtaataaagt gtttattata aacaaaattc aaaataaata atatctacat ataattaatt
     2221 ttagtgaagt atatctagtt taactagaat gtatacttca ctaatttatt tattcaaaat
     2281 atttatgagg attttaatat attaactaaa atatatcaat tgaattaact tttaaatctg
     2341 gaaaagcact tgaaatatag tgttttatgt catttaaatg gtcttgaatt tgcttttctg
     2401 atattttatc aggtccatat acaaaataca taatattttt agtatctttt gttatagctg
     2461 ttcgattatc agggccatta agaatactag ataatatacc aatattatct gataaaaata
     2521 aatcatcttt tgttaaaata tgttctttac cacctatacc ataaaaatgt tcacttccat
     2581 ttgctaattc tattttcaaa ggtaattcca ttttatcaag gtcatgacct gctgttagaa
     2641 gaagattttt caattctgct aaaaacattg actcaattgt tacacctaca tctgggatac
     2701 cttttgattt taatagaata gattctaatt gaagcaatac agggtaagtt ttcttgaatt
     2761 ttttataata ctttatataa cttgaaacag gctctgtttt aattttttct ttacgattgt
     2821 attctgggtg ttgttctata atatttttaa ttgtagagtt tttaagaact aaaaaatttt
     2881 ctttattagg ttttgaatgt gtaatattta taaccataac tccaaattta atattgggat
     2941 aaatttcttt aactttatgt gatatttcta acataatata gactcctttt tattttatat
     3001 tttatgatat aatcttagca aattaaaagt ggattttgta gttccatttt tatagtaatt
     3061 ttatagaacc acttttggag gtattagaat gtggattacg attaatagaa atagtaatat
     3121 atcattatca agacaaattt attgtgagat aaaagatatg attctggatg gaagcttaaa
     3181 gtttgagcaa aaactgccat catctaggac tttatcaaaa gaactatctg tatctcgtaa
     3241 tacagtttta gatgcatata atcaattaat tgcagaagga tatttggaaa caaggaatgg
     3301 atatggaaca attgtagcta aaggaatatc agggcataaa gttgatttta agttaaatga
     3361 tacttttaga gatgaaaaag atttacatac taataaacaa aaaaattata tagattttcg
     3421 ttcaggagtt ccagaattaa aactatttcc acgcaaagaa ctttcaaagc tatataaaca
     3481 aatactgtat gatttatcag attcggattt cagatatgga agtacagctg gtgtatgggt
     3541 tttaagagtg gaaatatcta agtatttatt tcgtactaga ggaattagat gtaatcccaa
     3601 aaacattatg attgtttctg gttcaacaca aggtttatct ttaatttcac agcttttata
     3661 caaaagtaat aaaaaggttg ctgtagaaga ccctattcat aaaggcttat taaaggttat
     3721 ttcatctatt ggatattcag ttgttggaat taaagtagat gataagggaa tgaataccaa
     3781 cttaataaaa tcaataaagg ggatttcttt tgtttatact acaccttctc atcaataccc
     3841 attaggaggg attttaccta ttcaaagaag actagagctt attaaatgtg caacagagaa
     3901 tgattactat ataattgaag atgattatga tagtgaattt agatatgagg gtcaaccaat
     3961 tagttctctt tatgaactta gtccaaataa agtaatttac ataggttctt ttagtaaaat
     4021 attatctcca gctattcgtg ttggatttat gttattacca gacaatattt tagaaagcta
     4081 taaaacctta aaaatgtact ctgatgttca tacagaaaca atattgcaac atgtattagc
     4141 tgaatttatt ggaaatggag gtttagagaa gtacatattt agaatgaaga agttgtacaa
     4201 tagaaaacgt aagtacctca taaaagtttt aaacacggaa tttaaaaatg aatttaaaat
     4261 aaaaggtgaa gcagcagggc ttcatatagt tgctcatttc tataatgtta aatttacgga
     4321 aagtttaatt cataaaattt ttgaagcagg tataaaagtt tatcctatac aacaatatgc
     4381 aattgagaat atattagaat atgactatga aattatacta ggttatgctc atcttagttt
     4441 tgatgaaatt gaaaaaggtg taaaaatact cagcaaaata atacatatga atcaaggtac
     4501 atagataaaa catgtagcaa tctacatata atagatgtaa ttaagtttaa actttaaaag
     4561 aaatatatat taatattttt gtataaaaat acctgatatt tttttatcga tactttattg
     4621 ctatacacta aattaagata gcaatttata ttaagaaaaa actttttgca taggttataa
     4681 tacttaaaag gagaggatag agttgaaaac aaatacagat gttattatgg agataattga
     4741 ttatatagaa gaaaatctta cagaaaatct aaatttagat aaaatagctt atattgctaa
     4801 ttattcaaaa taccatttac acagaatgtt tacaaatgtt gtaggatgta ctatccatca
     4861 atacatacag aggaggagac ttactgaagc agctagacaa cttatattta cagacaaata
     4921 tattatagac atagctatta cagtaggata ttctacacat caatctttta cactagcatt
     4981 tagaaattta tataaagaat ctccacaagt atatagaaat aaagatgaat tttatcctat
     5041 tcaacagaga tttgttttaa atctaaatca tcaaattcaa agtgaaaaat gtatttctta
     5101 tgcaaatatg aattataaac aaagaagaga tgaaatcaat tcttatctga actttaatat
     5161 taattgtgag gtgaaagtag catgagttta gtgccacatg taattgaaca aacaggtcaa
     5221 ggggaaaggt cttatgatat atattcaaaa ttattaaaag atagaattat atttatagga
     5281 gaagaaatta atgatgctat tgcaagttta gtagtagctc aattactgtt tttagaatca
     5341 gaagacccag attcagatat aattatatat ataaatagcc ctggtggctc tgctacatca
     5401 ggatttgcta tatatgatac tatgaattat gtcagatgtg atgtatcgac tatttgtatt
     5461 ggtatggctg caagcatgag tgctttttta cttgcaggag gaacacatgg aaaaagatgt
     5521 gctttaccca attcagagat tatgattcat cagccaatgg gaggagctaa aggtcaagca
     5581 acagatgtaa aaatagctgt agataatata ttgaaaataa aagaacgatt agataaaatt
     5641 ctttctgaaa atacaggtaa atcaatagaa gatattagga gagatacaga tagagataat
     5701 tttatgactg cactagaggc taaagagtac gggctgattg attatataat gaataggaat
     5761 gaataaagta tcaaagtatc atattaaaat gtctgtttta caaatatata tataatatta
     5821 aataagagtg tctcaaaatg aactttttag tttatgggac actctttttt gtatgaaatt
     5881 aaatatggtt ttatcatttc gacaattatt accaaatttc aagtttaata aattctacta
     5941 taaaaaatac ataaaagata aggttcttta gtaactttct gtcgtcaaaa gtttttataa
     6001 atttaataac aaaacaagag aaaataccat atattactta tgtctgcaat atatagaaga
     6061 caaaggagtt gtacattatg attacaataa gtttgtgcat gattgttaag aatgaggaag
     6121 aagttatagg cagatgctta gaatgtgtaa aggatatagt agacgaaatt ataattgtag
     6181 ataccggttc tacagattcc acaaaagaaa tagtctcaaa atatacagac atggtgtacg
     6241 atttcgaatg gatagatgat ttctctgctg caagaaattt ttcattttct aaagcttcta
     6301 aagactatac tatgtggctt gatgcagatg acataatttt agaagctgat agagaaaaat
     6361 tattaaagat taaagaaagc ttggatactt ccattgatat tgttatggca aaatacaatg
     6421 tatcctttga tgaaaatggt aacccaactc tctcatatta tagagaacga ttgtttaaaa
     6481 gaagttctaa tcataaatgg gttgacccta ttcatgaagt agtacctctt tttggagagg
     6541 tattttattc tgacattgca atatctcata aaaagcttca tagacaggac cctctaagaa
     6601 atcttagaat ttttgaaaaa atgatttcag aaggaaaaac attggagcca agacaccaat
     6661 tctattattc tagagagttg tactataatg ctaggtataa agaagcaata gaaggtttta
     6721 ctaagttttt agactcatca agaggatgga ttgaggattg tatcagtgct tgcagagatt
     6781 tagccacatg ttattactta attaatgatg aaaaaagtgc cttatattca ttgtttagaa
     6841 gtttcgaatt tgatgagcca agagcagaaa tttgttgtga cataggaaag catatgtttg
     6901 atagacaaaa atataaagaa gccatttttt ggtataaggt agcattaaca agagataaga
     6961 atgatactga tggaggcttt aagtcagatg attgttatgg atatatacca tctatacaat
     7021 taagtgtctg ttatgacagg ttaggagaat cagataaagc tatttattat catgaaaaaa
     7081 caaaagaaat caaacctaat gatagtgctg ttttgcacaa tgaaaattat tttagtaaat
     7141 ttaaaaatag ttgatgggtc acaatcatac taaatttgac atattatata tagagcttaa
     7201 aagctcaaat atatcaggag gtgcttttaa taatgagtag aaataaatat tttggaccat
     7261 ttgatgataa tgattacaac aatggctatg ataaatatga tgattgtaac aatggtcgtg
     7321 atgattataa tagctgtgat tgccatcatt gctgtccacc atcatgtgta ggtccaacag
     7381 gcccaatggg tccaagaggt agaaccggcc caacaggtcc aacgggtcca acaggtccag
     7441 gtgtaggggc aacaggccca acaggaccaa ccggtccgac tggtccaaca ggaaatacag
     7501 ggaatacagg agcaacagga ttaagaggtc caacaggagc aacaggggca acaggcccaa
     7561 caggagcgac aggagctata ggatttggag taacaggtcc aacaggccca acaggagcga
     7621 caggagcaac aggagcagat ggagtaacag gtccaacagg tccaacggga gcaacaggag
     7681 cagatggaat aacaggtcca acaggagcaa caggggcaac aggatttgga gtaacaggtc
     7741 caacaggccc aacaggagca acaggagtag gagtaacagg agcaacagga ttaataggtc
     7801 caacaggagc aacaggaaca cctggagcga caggcccaac aggagcagta ggagcaacag
     7861 gaataggaat aacaggtcca acaggtccaa caggagcaac aggagcagac ggagcaacag
     7921 gagtaacagg tccaacaggg gcaacagggg caacaggagc agatggagta acaggtccaa
     7981 caggggcaac aggagcaaca ggatttggag tgacaggccc aacaggtcca acaggagcaa
     8041 caggaatagg aataacagga gcaacagggt taataggtcc aaccggagca acgggagcaa
     8101 caggagcaac aggcccaaca ggagtaacag gggcaacagg agcagcagga ctaataggac
     8161 caaccggggc aacaggagca accggagcag atggagcaac cggagcgaca ggggcaacag
     8221 gagcaacagg tccaacagga gcagatggat tagtaggtcc aacaggagca acaggagcaa
     8281 caggagcaga tggattagta ggcccaacag gtccaacagg ggcaacagga gtaggaataa
     8341 caggagcaac aggagcaaca ggagcgacag gtccaacagg ggcagatgga ttagtaggtc
     8401 caacaggagc gacgggagca acaggagcag atggagtagc aggtccaaca ggagcaacag
     8461 gagcaacagg aaatacagga gcagatggag caacaggtcc aacaggggca acaggtccaa
     8521 caggagcaga cggattagta ggtccaacag gagcaacagg agcaacagga ttagcaggag
     8581 caacaggagc aacaggccca ataggagcaa caggtccaac aggagcagat ggagcaacag
     8641 gggcaacagg agcaacaggt ccaacagggg cagatggatt agtaggtcca acaggagcaa
     8701 ccggagcaac cggagcgacc ggagcaacag gtccaacagg agcaacagga gcaacaggag
     8761 caacaggtcc aacaggtgct agtgcaataa taccttttgc atcaggtata ccactatcac
     8821 ttacaactat agctggagga ttagtaggta caccaggttt tgttggcttt ggtagttcag
     8881 ctccaggatt aagtatagtt ggtggagtaa tagaccttac aaacgcagca ggaacattga
     8941 ctaactttgc attttcaatg ccaagagctg gaacaataac atctatttca gcatacttca
     9001 gtacaacagc agcactttca cttgttggct caacagttac aattacagca acactttacc
     9061 aatctactgc accaaataac tcatttacag ctgtaccagg agcgacagtt acactagctc
     9121 caccacttac aggaatatta tcagttggtt caatttctag tggaattgta acaggattaa
     9181 atatagcagc aacagcagaa actcgattct tactagtatt tactgcaaca gcttcaggtc
     9241 tttcattagt taatactgta gcaggatatg caagtgcagg aattgcaata aattagatta
     9301 gataagaaaa ggcttctaat atcgtattga tattaggagc ttttttaaga atgattgatg
     9361 aacatagttt taatattaat tagtgcgcat attataaaat actattatat aatataaaat
     9421 aataacatta aattggaaat atagaattat ctaggggtga tactatgaaa aataagattg
     9481 aaatatcaat gaacaacttt gtaaaaaatt atcataaatc aaataatata actacaaagt
     9541 ggcaagaact tttgtttgct tatgcatctg ctgaagatga tatgtttcag aagctaaaaa
     9601 gtgttgtagg aacatctcat gctatgccaa aagattttct ggaaaatgga aaaactataa
     9661 ttacatattt tatacctttt gatgaaagtg taaataatag taatattgga gggtttgaat
     9721 gttctgaaat atgggcaaga gcgtacatag aaactaatat gttgatttca aatttaaatg
     9781 atttcttatg tgaagaacta gagaagatgg gggctaattc atatgctatt cctgggtcgt
     9841 ataattttga cagtgaaaaa ttggtaagtg actggtcaca aagacatgta gcattcatag
     9901 caggtcttgg aacttttggt ttaaataata tgcttattac agaaaaaggg tgttgtggaa
     9961 gaataggaag cataatcaca agtttagaat tagagccaac taaaagacca gaaaatgaat
    10021 actgcttgta taaacataat aatagttgca aaaaatgtgt caacaaatgc gtgaaagaag
    10081 ctttaaaaga agatggattt aataggaata agtgttatga aatgtgtatg ataaatgaaa
    10141 aaaaatacac ttatataggt ggatgtgata tttgtggaaa gtgtatagtt ggagttcctt
    10201 gttcagtaat aaacccatca agataatgga tttaagttga ctaataattt taaagatagt
    10261 ttataatgaa aaaattttag ttatcaaaat aaatataata taaaaactaa ataaatgtgt
    10321 ttaaaaaatg gataaataat ttgtgttgat tgacaaccag ttatactgtt gatagactta
    10381 tatgtatcgc tgtgatatat aaggagggtt gagattttat gtttgattta atctttattt
    10441 ttgcatagca agggctattg caactatgaa atttaatata taaatagccc tttgctattc
    10501 ctaatgaaaa attactattt aataggagag caaaatgagc tataaaaaag caatacatat
    10561 attaccagaa gaattacttg aattaataca ggaatatgta gatggtgaat gtatctatat
    10621 tcctagaaaa tctaataata aaaaagaatg gggaagtaat acacctactc gtgaggagtt
    10681 gtcaattaga aatatgcaga tttacgaaga ctatcaagtt ggatatgatt tgcaatattt
    10741 atcggaaaag tattatttat ctttgaaaag tatccaaaga attgtattac aagaaaaaag
    10801 aaaagatatc taaggatgag ccagtatagt tttataatta tactggctca ttttcttttg
    10861 ataaaagtgt ttgaggtata gtttctttta agtggatgat ataatttaag taatttcaat
    10921 aacttataca gtagctaaca aagctggttg taaagtatct aataagaagt agtagctttt
    10981 tatatgtttg aaattaaaat ttattaaaat taggaggtct tgttttgaaa aaaatacttg
    11041 attatattcc accttttcat aactttctat taatttagta atcttataga aaggaaattg
    11101 catttatgaa aaaaattact tgggaggtgg aatatatctc ccagttacct gtaattaggt
    11161 attgtaaaaa atgtggaaaa aagactgaat atatttgttc aggtttattt agggttaatg
    11221 ctcaacataa atatttggat atatggttaa tatacaagtg ttcaaattgt gattcaacat
    11281 ggaactcaac tatatattct cgtgttaatc caaaaagctt aaatccagaa atactagaac
    11341 aattccatac aaacgattat aatcttgtta agcaatatgc tatgaatatg gaacttttac
    11401 atagaaatgg agcagaagtt ggtattccag agtataaaat agtaggacaa gaaattaata
    11461 ttaatgacct tacacaagtt catattatta gtaaatatcc atgtcagtta aaggtatcaa
    11521 cattattacg tgagaaacta ggtttatcta ggaaggtatt tgacgaaatg ttggattgtg
    11581 ggattattag aagtaactca ggagttgatt taaagaaagg taaattaaat tatgaaatta
    11641 tgttagatat aggcaaggag aagcaaaagt cttcaaattg attagaataa ctaacttaat
    11701 atattttcag gaacatcatt ta
//
LOCUS       DAYASV010000117        11513 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYASV010000117 DAYASV010000000
VERSION     DAYASV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.119"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            140..559
                     /locus_tag="ACGI68_002956"
     CDS             140..559
                     /locus_tag="ACGI68_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139382.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            584..5641
                     /locus_tag="ACGI68_002957"
     CDS             584..5641
                     /locus_tag="ACGI68_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFH0139383.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLILLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSNLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(5826..6563)
                     /locus_tag="ACGI68_002958"
     CDS             complement(5826..6563)
                     /locus_tag="ACGI68_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0139384.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(6582..7517)
                     /locus_tag="ACGI68_002959"
     CDS             complement(6582..7517)
                     /locus_tag="ACGI68_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0139385.1"
                     /translation="MDNNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRDILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRLHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            7725..10301
                     /locus_tag="ACGI68_002960"
     CDS             7725..10301
                     /locus_tag="ACGI68_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139386.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YTNKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            10313..10984
                     /locus_tag="ACGI68_002961"
     CDS             10313..10984
                     /locus_tag="ACGI68_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139387.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            11011..11352
                     /locus_tag="ACGI68_002962"
     CDS             11011..11352
                     /locus_tag="ACGI68_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139388.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENEDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
BASE COUNT         4480 a         1478 c         1681 g         3874 t
ORIGIN      
        1 actttaagat agcttttttt ctattttttg taaaataatt ccataattca cattaataat
       61 tcacaaaatg atataatata acaaatagat aaatttttaa atttctaaat acataattaa
      121 tattttgcga ggtgatttta tgcaaattta ttttagagtt aaggctgttg gcaaacgcaa
      181 acctatgcta gaacttaccc cttttgatgt atctgacaac atcaattgcc taaaagaagc
      241 tatctctgaa attgtatcta aaaatgttct tgaatataat gagaaaatcc cagaaaaatc
      301 aataataaac ttcttaacta atgaagaaat tgaatctcaa gctgatattg gaaaagttgg
      361 atttggctct atttacaatg agaataaaca atctgtagaa aaggcaatag aggtagcgtt
      421 acaggcattt gaagatggaa tctacaaagt gcttataaat gaggatgtta ttgaaaaact
      481 cgattcctct ataaatctga aaagtggaga tatatttact tttataagac ttaccttgct
      541 tgctggaaga atgttttaaa ataaataatt tggaggtaca taaatgttag aacgtactag
      601 agtagaaaaa aatcctaaaa taattgaaaa ttttaaaaat aattttacta aatcttcttc
      661 aaaacagtac tacgacttag caaagtctct ttgggactgc atagaaaata gttcatacta
      721 ttatagagaa cctgaagaca aattcttaaa tatgataaag aaatactaca attgccacta
      781 ctacgaaatt aaaaatatgg acgagctcct ttctggacct ttaaaaaatg cattagaatt
      841 aatatttact tctgaacaag tagaagaact ttgccaatta attaaaagaa agatggatta
      901 tacatattca ggaggatatt atcgtaaagg gtatcgctca aataaacttg tggattattt
      961 tagaggtatt ataaggttta tttcaaacta tgttgattta atttattatg atttttcagt
     1021 tatagattat cttactcttc caaataaggt cttgtactat gactctataa tgagtgataa
     1081 aatagcacta gaaatagaca aaggaaatca aaaagtaata agtgctttag aagaagtaat
     1141 atttggagaa aatcaaactg ctctacttac aagacaaatg ataattggta tcatgaagag
     1201 tcatagtgat aaatgctatg aaatgctttc taaacttctt ttggcagcca aacaacaaga
     1261 aggacttaga cagcaaattg tagagagttt agatgagggt acaatttctg catcaatata
     1321 catgttaaaa actattttgg acaacaattt agagcgattt agttctgtta taagagcctt
     1381 ggatacatgg acaggacttg gtttttcaga ccaaaaacct ggaataatac gtaaatgttt
     1441 agaactttca tataaatcat tgactgatga aaagttccgt atagaatcaa ttaatagcaa
     1501 tgataatctt gaaatttatt ttgccttatg ggctactgca acttacaaca tatatgactc
     1561 ttactctctt attgaaaact tactttcttg tcaggaaaaa tataaaaaac ttgtagctct
     1621 ttacttttta tcgcatacag aaagtacata ttttaaacat tctatagcat ctaaaaacat
     1681 taagggtgat gatttagaga tactagcatg ggtaattaca aatttctatg tagaaggttc
     1741 tgttggctac tactatagtt ggcaaaacag agaaactaat tttgacttgt caaacactcc
     1801 tattgacaaa gactttgatg ctagaaaaga acaatttgaa agtttaaaac atactttaga
     1861 aattttaaat tcaaagacaa aatcattttc aggaagtgta tttccatgga cttggattga
     1921 aatttcttct catgaaatac ttaaatgtat gatgacttta acagcatacg atatgaatgc
     1981 tcaatttata gatgtgctta tggaaaattg tcatcttatg agttcagatt taagagcttc
     2041 acttatagtc aattttgtaa atgaaccaaa gacagataga caacgtgaat ttttattgga
     2101 atgtttaagt gataagagtg tttccaatag agaaacggta ttgagtctac ttagaaagct
     2161 tgagctaaca gatgtagaat tagataagat aaccaatcta ttgtctttaa agacaagctc
     2221 tattagacaa acagtaataa aaattttact tgaacaagat gattcaaatt tggaaaatat
     2281 tgcttcttca ctgataaaat cttcaaattc tcaaaaatgc cttggaggtc ttgaaattgt
     2341 aaacttatta aaagacaata ctgctagaca agaactatat aataagctta ttttacttat
     2401 agaggatatt gataaatcta aaaagaatac atctcaagtt caaatactaa ttgataattt
     2461 gttaggtaaa gcaaaaaatg attactgcaa agaaaatggt tttggaatat ataaccctga
     2521 aaacaaagtt caaattccag aacaatttga ttttaaagat gattctaata gctcatcaaa
     2581 gcttaataaa attatctcaa atataagttt atctactcgt gaggattctg tttctgtagc
     2641 aacaaaaaaa gaagcaaaaa aaattctcga tacggatata aataaaataa taaaagttta
     2701 tgaaaaattt gatgaactta ttactgaaaa tgcagattat gaatatgaag ttgaaagatg
     2761 gtcaggtaat gtagacaaag tacttttagg ttattctact tataacttga ctcctattaa
     2821 atataaagtt tctaatgata ctcttgataa ttatccatta gcagagcttt ggagagaaac
     2881 tagtaaaaaa cttgacttag attacaaatt aatacttgat atcttatttt atagtataag
     2941 tacatgtgga tataatgaaa cagaaaaaag ttggttttca tctatatatt caaatgtcct
     3001 tgactctaaa aaacaaaata aaatatatac aaaacttgaa aaacttaaat ttaatggact
     3061 tacaagaaaa cttttaagag ttttactaaa tgagtttgac cataaagaaa tatttaacta
     3121 ttgtttagaa atatactata aacttagaaa cttattgact atagaacaat gtaaaaaaca
     3181 aagagttgaa tttactaaaa actattacag acaaaatgaa tactgccctg ctttaaatgc
     3241 ccctatttta aactattggc ttgataaagc acattcgttt atttatgatg atatttcttt
     3301 tactaagttt ttctacacta cgttttcaaa ttatcagttg ctagaatata agtttccttt
     3361 ctatactagc ataaatgtgt ttgctcgtgc acatgaatta ggattaatga ctgaagagca
     3421 agttaataga gaaatgataa gtggatacaa cgcatcaaat ataataagaa cattgacaaa
     3481 tcctagatgg gctggcgaca cattagaaaa gtacccttgg cttaaagata atttaaaaac
     3541 tgttacaaac cgaattatag aaattgaatc acgtagaggt gatatgccaa cagaagtgtc
     3601 taatctagct aataatatac aatactttga aggtataaat tattttgtaa atattttggt
     3661 cgctcttggt aaagaaactt tccagagagg atattcatat ggtagcaatt attcaaaaaa
     3721 agatgtttta agttctctac ttaaaaattg ctatcctact aaagaagatg attcaaaaaa
     3781 attaaaaagt ttacttaaaa ataccgacat tactgagtta agattgatag aatctgctat
     3841 gtatgcccct caatgggtaa acataattga aaaatattta aactggaagg gtctgaaaag
     3901 tacaatttgg tacttccatg cacatataaa tgaaggcttc tctgcacaaa aagaatcaga
     3961 ggttgctcta ttctccccta tttcaccacg tagtttcatt gatggtgcat ttgatgtaga
     4021 ttggttttta gattcatata aaactattgg ggaagaaaga ttcaatatac tttacaagtc
     4081 agctaaatat ataactagtg gtggtaactc tcatagacgt gcacaactct atgctgatgc
     4141 tacacttggt aagcttgata aagacatgtt gatgaaagaa attgaaacaa atagaaatca
     4201 agataaattg agaagtttct ctttacttcc tatgaaaaaa ggagacctaa aagaagcaac
     4261 tttccgttat gaatttattc aaaagtttgc taaagaaagc aagcaatttg gtgcacaacg
     4321 tcgtgaaagt gaatcaaaag cttgtactat agcgctagaa aatctagcta gaagtgctgg
     4381 atttggagat gtaaatcgta tgatgtgggc tcttgagact gaaaaactaa atgaagttgc
     4441 tcatctattt aaaccaaaag atattgatgg tacttcaatt tggatagatg ttgatgaaaa
     4501 cggaaaagca tctactaaag ttcaaaaagg caacaagtct ttaaagagta taccaaaagc
     4561 tattgctaaa catccatatg tacttgaact taaatctgtc caaaaagatt taaagagtca
     4621 gtattctcgt gcaaaacaga gtttagaaca agctatgaaa aatgaaatag tatttacagt
     4681 taaagaaatt gccaatatct ataaaaatcc agttatctgc cctttgatta aaaatctagt
     4741 atgggtatgt gatgataata ttggatatgt ttataatgat aagtctaagt tctttatttg
     4801 caatatagaa ggtgaaactt ataagttgac ggcaaaaaat aaattaaaac ttgcacatcc
     4861 agttcatttg tttgaatctg gtaagtggag tgagttccaa catgatttat ttagtaagca
     4921 aattgttcaa ccatttaagc aagtattccg tgagtattat agaataaaca gtgatgaatt
     4981 atctgaaaaa actatatctc gcagatatgc tggtcaccaa attcagccaa agaaaactgt
     5041 agcacttctt agaaatcagc tttggactgt cgattatgaa gagggattac agaaagtatt
     5101 ttataaagaa aatatagtgg caaagatgtt tgcacttgca gattggtttt caccagcaga
     5161 tatagagcct cctactattg aaacagtaga attttttgat agaaaaactt ataaaagttt
     5221 agaattagct tctatacctc caattctatt ctcagaagtt atgcgtgata tagaccttgt
     5281 tgtaagtgtc gctcatgctg gtggtgttga ccctgaaact agccattcta ctgtagagat
     5341 gcgtatagca attgctaaag aacttttatt attattaaaa attaacaatg tcaattttga
     5401 aggctctcat gctcttatag caggtaattt aggtgagtat tctgtgcata tgggttctgg
     5461 tattgtacat aaaaaaggga tgggtgcttt gaatatactt cctatacatt ctcaaagtag
     5521 aggtaagata ttccttccat tcgctgatga agaccctaaa actgctgaga ttatgtctaa
     5581 gatactatta cttgctgaag atagtaagat taaagaccct agtatcttaa gtaatttata
     5641 agtataaaaa ctatctctaa aatatattgg actttcttaa aaaaattaga aagtccaata
     5701 tatactatac tgtaatttat atagattaaa tcataattac aaataatttt tatataaact
     5761 tctcttatac attatcttta cggtacttat tatatcacac aaaaagtatt tttcttctat
     5821 cttcactaga atcttttaac cctataaaat ctatataaaa aatctttttc tagagatttg
     5881 tttaatatgg cattttcttg aaaatttata aaccttatct ctaatgaaga tttatttata
     5941 ggtataacct tatgtataaa tcttagattt actatgtatt gtctatgggt cataaagaaa
     6001 ttaggaggta ggcgtttttt taaatcatca aagttctcat aaaactttat tatatcttca
     6061 tctttagtgt gtagattaat tgctcttgaa tttttctcaa acataactat atcatcaaaa
     6121 ttaatcatat gtatctgctt ccttatttta tatacaaaaa gggtgtcttc tataaaatta
     6181 ttattagcaa tcttatggga gttaatatgg tctattgcta tgttaattga atcaagtatt
     6241 ttttcttttt taaatggttt aactacaaaa tctataggat gcactttaaa gctttcaaga
     6301 ctataatcag cgaaacccgt cacaaatact atatgtatat ccttatctat agaaagaatt
     6361 tcttcagcag aatcaatccc atttttatat ggcatatcaa tgtcaataat caataaatct
     6421 attttctcat tttttataaa ttcaattgcc tctataccat ccttaacttt tttaacataa
     6481 tctacatatc cagactcttt agctatattt gctatatcat ctcttacaat cttatcatca
     6541 tcacaaatca aaacttttat catagacttt tctcctccta actaataata ttttgggatt
     6601 aagattacaa attcagttaa aaaatcatca ctatttacaa gtatactata atcatactta
     6661 tctaatatat ttttaactaa atgtagccca tatcccctat cttgtccttc tttagtacta
     6721 aatccattcc aaaatatact ttcaacatta tgtattttag gaccattatt ttttatagct
     6781 ataatatact gaaattcatc ttcatatgca tgtaatctta tatacttctt ttcttttttt
     6841 atagtagaca aagactctat tgcattatct attatgttta atagtactga actcatatca
     6901 ttaggttcaa tctctaaatt atccataaaa tcttgaatgt agacatcaaa agatatatcc
     6961 ttttcaagac attcctcgta ttttaatgaa gctatagcat ctaaataaga tacttttcca
     7021 taccttgaat tttttgattt aaagctattt gatatattca ttatatattc tttggcatct
     7081 tcatctagcc cagtatttaa tagagaatac actacttgca aatgtttcaa atagtcatgt
     7141 ttttgacttc ttaatttgat aattatatta tccttatgat ttattttact atctagtttt
     7201 tgtaatttat attcctgttc attattatta tttatcaatc ttataacttt aaagcctgta
     7261 taaaaactta aaataattaa aaccaaaaaa agtataatat ttaatactcc ttcaagtaaa
     7321 aaactatatc ctaaccttaa ttgtatatat ttagtgccct ccataaataa aatcaatgca
     7381 aaacacgata taaaaaatgt taatatgtct ctttttaata tgtctaattc tttgtaaaat
     7441 ttatacttat tcattataag attgctaaat ataaacaaag ttagtattac tataggaaat
     7501 acaaaattgt tatccatgat tcccctcccc tctacaattt atatagggat tatatcattt
     7561 taaaaaaatt atgtaaaaag ttaaaattta catatatatt aattaaactt gtaaaaatac
     7621 agatgaattt gtacaattaa ctacttaaaa tttgaaaata cttactttgc tatatatttt
     7681 agataaatat ataattgatt tagttaagaa aggaagtggt aaatatgaaa gataatttga
     7741 aaatagtact aaggtatata aaaagttata aagctaggtc tttagcaatt gttgtgagta
     7801 taattcttgc tacatcactc gttgttggta ttggtactct ctcaagaagt gctcagcaag
     7861 cagatgtaga tagattgaaa agagagcttg gaactgacca tgtatacttt aaaaatataa
     7921 agaaaaatca attaaagtcc ataaaatcta gcaaagatat taaaaatctg ggtataacat
     7981 cttactatgc gtctacagat gttaatgaaa aattacctat aaacatacta tatacaaata
     8041 aaaattattt gactagtcat tctaaactta taaaaggtcg atttccaaaa gctagcaatg
     8101 aggtggtagt cgagaaatgg attttaaata gtatgggctt aaaaccagag ataaatcaaa
     8161 accttacttt taaattatat caaaaagaaa atccagaaac ttttaaagtt gttggaatcc
     8221 ttgaagatag atttatagag aaaaaaaagg gacaatgtga aattttttct aatctcaatg
     8281 aatcagaact gaatgatttt acaacttata ttgaatttaa tgaaggcagt gatataaata
     8341 caaacataga caatataata aaaaatgcta tgttagatga gaaaagtata ggaagaaact
     8401 caatgcttat agaatctact atgaaaaacg gtactttaga taattcaagt aaatacgttg
     8461 caattgctat gagtctattt gctggtattg taatatatag tatctatgtt atatcaatat
     8521 accaaagagt acaggaatat ggcgtcttaa gagctattgg ttccaccaac tttaggattt
     8581 tcaaacttat gctttatgaa cttttcatac ttgctctaat agcaatgcct ataggtatat
     8641 gtataggtat gggaggtgca caaatattta ataggtctgt aggaaatatt caatttgaag
     8701 gaaatataaa tgttacacct tttgttattc cagataaaat aattctctta tcaattgcat
     8761 gtacaatact aataatacta attataagtt tctttacata cttaaaaata agaagaattt
     8821 ctcctattga tgctatcaga aaaacttttg gagctgataa aaatgtaacc aaagttaatt
     8881 atttaatatc aaaattaact ttaaatatgt cagtaacaaa atctatatct gcgaaaaaca
     8941 tctttagaaa taaaaagggg ttcattataa ttgtattatc tatgagtata ggaggtataa
     9001 tcgtcataaa agacaattat aaatattctt ttttagatgt gcaaaacaaa tctactcaag
     9061 aacaaacata tatgaatgca gattttattc tatcaaatgt ttatctcaat gaaattggta
     9121 acaatacttc ttttaaagat ataaaagggt ttaatgataa tcagatagat aaaataaaaa
     9181 atatagatgg aattgacaaa gtcaaaactg ctagtatctt aaacacaaga atagaggtaa
     9241 ataaaataaa tagattagat tactacgaag ctataaattc aactccttat tataagctaa
     9301 atcctctgtt cataaaaaat aagactactg gcaaatatac actaaagcaa aagctaaaag
     9361 gttataatga tgagatgata aagtcattag aaaaatactt agtaagtgga aatataaact
     9421 tgaaaaaaat gaaaaaagaa aatttagcta ttgtatatgt accagaaatt tctaaaacac
     9481 agaaatacac aaagactttt actcctggtg aaggaacacc agctgtagat ataaaagtag
     9541 gtgatactat aaaagtcaaa tatccaaagg gtgggatgga tacaggtctt tatgaaagtc
     9601 taaaagataa ttatgaatac ttagaatatg aatttaaagt tggagctatt ataagttatc
     9661 cttttgcaga taatggtatg tactctggag atgatggtgt agatgtaata acgagtagcg
     9721 aatacctaaa aaaattaact ggtgtagata agtataactt agtttatgca gaccttaatg
     9781 aaaatgcaaa caagaaaaaa ataaataacc ttttaggtaa aattggaagt gaagttcctg
     9841 gaactacaac agtagatatg atggtagaaa aagaaaattc tgataagatg acatctagaa
     9901 ctatgattta tagttatggt atagtagcaa taatgttcat aatcagtgta cttaacataa
     9961 taaacaacat aagttataac ttaacttcaa gaactagtga atttggaatg ttaagagcta
    10021 ttggtatctc tgagagaggc tttaaaaata tgatacttta tgaaggaata ttatatggtg
    10081 tactctcaag tataattact atcgtgtcag gaattataat acaatttaaa atgtattata
    10141 tgcagggctt tgtatcatat ggattgggat tttcaataga ttacaaaata tatatactag
    10201 ttgttgtagc aaatataatt gtaggtattc tagctacata tattccatca agaaagataa
    10261 ataaaattag tatagttgaa gctataaaca ttacagaata ggaggaataa ttatgcctat
    10321 attggaaact attaacttag gtaagattta tggaaaaaag gaaacatctg tacatgcact
    10381 aaagaatgca aatttaaaaa taaataaagg cgaatttgtt gctataattg gaccaagtgg
    10441 aagtgggaaa agtacatttc tacatttagt aggtggtctt gaaagaccaa gtaatggtac
    10501 tataaaggta gatggtaaag atatatgttg tctttcagat aaagaattag caagatatag
    10561 aagacaaaag gtaggatttg tctttcaaca gtataactta attcctgtat taaatgtaaa
    10621 ggaaaatata gaattaccac taaagttaga caataaaaaa atagataaag aatatattga
    10681 tgaccttata aatttattgg gtcttaatga aaggaaaaat catcttccca accaattatc
    10741 tggtggtcaa cagcaaagag ttgctattgc acgtgcttta tctgcaaaac caagtataat
    10801 cctagcagat gaaccaacag gaaatttgga cagtaaaact acagaagaag tcatggattt
    10861 actaaaaagt tctataaaaa aatataacca aacacttata ataataacac ataatgaaaa
    10921 tattgctaga aaagcagata gaattatatc tataatagat ggagagctta aattaacact
    10981 ctaaaactat ttatagaaaa gaggatttaa atgttattga ttttaggtaa tttaaattta
    11041 aacttggatt ttgactacag aataataaga gaagaaaatg aagatgtaga tatatttata
    11101 gatattaatt atcgtagctt agatatagat actgatggtt ctaacttatt taattctaga
    11161 atacaatttc catttgtaag agcattgatt ctaaggctaa ataagaacaa tcaatgtatg
    11221 acaattcatt tgttaagaga tatagattta ttctctgctt ttgccaactt tgaagtggat
    11281 tatactgact ctataataaa tataaaaaat caaaatgaga aagtaatttt aaataaatct
    11341 atcaaaaaat agtaaggggg ccttaatagg ccccaatttt tttcttttct aaacttatta
    11401 tatcatgata aataaattag tcaatagaat tttctgaata tttttataat tttttcataa
    11461 agttttttat atattatgct ttaaaattat atgtaataaa aaaattttcc gac
//
LOCUS       DAYASV010000118        11387 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYASV010000118 DAYASV010000000
VERSION     DAYASV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.120"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            332..913
                     /locus_tag="ACGI68_002963"
     CDS             332..913
                     /locus_tag="ACGI68_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0139389.1"
                     /translation="MMGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLD
                     TAAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPG
                     EISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVA
                     EIYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            952..1530
                     /locus_tag="ACGI68_002964"
     CDS             952..1530
                     /locus_tag="ACGI68_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0139390.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1616..2245
                     /locus_tag="ACGI68_002965"
     CDS             1616..2245
                     /locus_tag="ACGI68_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFH0139391.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2235..4994
                     /locus_tag="ACGI68_002966"
     CDS             2235..4994
                     /locus_tag="ACGI68_002966"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0139392.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     LKKTA"
     gene            5081..6643
                     /locus_tag="ACGI68_002967"
     CDS             5081..6643
                     /locus_tag="ACGI68_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HFH0139393.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6644..7780
                     /locus_tag="ACGI68_002968"
     CDS             6644..7780
                     /locus_tag="ACGI68_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFH0139394.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7844..8920
                     /locus_tag="ACGI68_002969"
     CDS             7844..8920
                     /locus_tag="ACGI68_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFH0139395.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHFITGILIPKANSELIENCIEALDFMNLQDMYVI
                     PIVETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8898..10136
                     /locus_tag="ACGI68_002970"
     CDS             8898..10136
                     /locus_tag="ACGI68_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HFH0139396.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            10190..11278
                     /locus_tag="ACGI68_002971"
     CDS             10190..11278
                     /locus_tag="ACGI68_002971"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFH0139397.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNISLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
BASE COUNT         4550 a         1152 c         2134 g         3551 t
ORIGIN      
        1 ggtagtagtg gcgaagacac cgtccgaagt agcttggctt tgtaaccacc tctgttgtac
       61 tgtttaagta taaataagtg tgttagccac caagaatccc cttgctttag ctatggggag
      121 tgtgtcaaat attaaacata gcttttatac atttaataag gtatgtaaaa tctatgtaaa
      181 aagtaagaaa tataaacatt tttttaatta tattttaaaa atatcaagca agttttcttc
      241 acaaatgaat aaaaaatgtt ataatatgat taaagaaaaa gataaataat tagcaagatt
      301 tgtttagcta aaattaacaa gggagcgata aattatgggt ataacattat caaaaggcca
      361 aaaggttgat ttaactaaag gaaatccagg tcttaaaaat attttagtag gtttaggatg
      421 ggatactaac aagtatgatg gtggatttga ctttgattta gatacagcag catttttaac
      481 aggagcaagt ggaagtgtta ctaatgatgg agattttgta ttttacaaca atttaaaaca
      541 tacatctgga gcagttgagc atctaggaga taacagaact ggcgaaggtg atggagatga
      601 tgaacaaata gttgttgatt tatctaagat tcctggagaa ataagcaaga tttcttttac
      661 tgttacaatt catgatgcaa cagaaagaag acaaaatttt ggacaagtaa gtaattctta
      721 tataagaata gtagataaag acacaaatga agagttaata aagtatgaat taggagaaga
      781 ttttagtata gaaacagcta tagtagtagc tgaaatatat aagcacaatg gagaatggaa
      841 gtttaatgca ctaggttctg gctttgaagg tggtcttgct gcattatgtg gaaactttgg
      901 aataaactta taatttcaac tataacaata aactaaaggg ggatattttc catggctata
      961 gtattaaaaa aaggacaaaa aatcgattta actaagggaa atccaggtct taaaaatata
     1021 aaacttggac taggatggga tacaaattca tttgatagtg gctatgatta tgatttagat
     1081 gtaagtgttt tcatggttgg agaatctcaa agagttgaaa aagatgaaga ttttatattc
     1141 tacaataact taaaacatcc atctggagca gttgaacact taggtgataa tagaactggt
     1201 gaaggtgatg gagatgatga ggaaatatta gtgaatttag agacaatgcc aaagcatata
     1261 caaaagatag ctgtaacagt tacaatatat gaggctgctg aaagaagaca aaattttgga
     1321 caagtaagta actcatatat aagagtatta aattctgaaa atgatgaaga aatattaaga
     1381 tatgacttag gtgaagaatt tagtatagaa actgcaatta cagtatgtga gatatataag
     1441 tataatggag aatggaagtt tagtgcagtt ggagcaggat ttgaaggtgg cttagaagca
     1501 ctttgtagaa actatggctt aaatgtttaa tttaagcaat ataatatatt aaatataaat
     1561 aacctataca aataatgtat aggttatatt atataaaatt ttgtggaggt ttagtatgtc
     1621 gattaattta tcaaaaggtg ataaaataga tttaaaaaaa tctaacccag gactgagtaa
     1681 catacttgta ggtctaggat gggaccctgt gcaacaatct ggtggaggtt tttttaaatc
     1741 gttatttggt ggaggacaag ctgatataga ttgtgatgca tctgtattta tgcttaatca
     1801 agaaggtaaa ttaagtggaa taaaagattt aatttatttt ggaaatctaa aaagtgcctg
     1861 taaatcagtt ttgcatacag gagacaatct aactggtgag ggtacaggtg atgatgaaca
     1921 aatcctagtt aatttggata aagttccatc aaatattcat aaattattgt ttgtagtaaa
     1981 tatatacaat tgtgtagata gaaaacaaca ctttggaatg atagaaaatg cctatataag
     2041 agttgaagac caaggtaata agaaagagat agcaaaatac aatctatctg ataattattc
     2101 tgaaaaaact actcttatag taggagctat atatagaaaa gatggaagtt ggcaatttaa
     2161 agcaattggt gaaggaacaa aagatgcggg tcttaaagaa gtaatgcaaa atttagacag
     2221 gattgagtgt gcatatggaa tttaataatg atatcaaata ttataatgta gataaagaag
     2281 aagttacaaa agacttatct gttaatcctg aaaaaggttt atcagaatca gaagtcaaga
     2341 ctagaagaga aaaatatggt ttgaatgaat ttactccaaa agaagagggt agtttttggg
     2401 atgatttaaa ggaaagttta agtgaaccaa tgatagttat cttgattatt gcagcagtag
     2461 taagtgcagt aattggggaa actcatgatg caatagggat tgtaggtgct atagcaatag
     2521 gtatagcaat aggtatgatt acagaaggtc gttctaagaa agcagcagag gctctttcta
     2581 agttgactga aaacatagaa gtaaaagtat tgagagatgg ggaagtacat caaatttcta
     2641 agagtgaatt ggtaccaggt gatatagtat atattgaaac tggtgacatg gtacctgctg
     2701 atggtagact tatagaaagt ataaacctta aaattagaga agatatgtta acaggagaat
     2761 ctgatgacgt atctaaaaag tgtgatgtag tagtttctat ggaagagata gaatctaaag
     2821 gagcagtagt agtacaggag ccaatacctg caaaacagat aaatatggtg tttggtggca
     2881 cacttgtagc ttatggtaga ggagctctag ttgtaacttc aattggagat aatagtgaaa
     2941 tgggtaaaat tgctcaaaac ttatcagaaa cagatgagca aactccactt cagataaaac
     3001 ttggaaattt aggtggtttg atagccaaag tttctagtgc agtagcagga ttactattta
     3061 tatttatggt tttccaaatg ataagtaaga atgtattaaa tgtagatatg tctggaatat
     3121 taccgttttt agaatcaata gacccagtta aaacagcatt tacagtatgt gttgcgttga
     3181 tagttgcagc agtgccagaa ggtcttccaa ctatgataaa tatgacactt gcaataacaa
     3241 tgcaaaaaat ggctaaaata aatgcattgg ttactaaaaa agaagcttgt gaaacgatag
     3301 gttctgtttc tgtaatatgt tcagataaaa ctggtacatt aactgaaaat agaatgactg
     3361 tagaagttgc atatgttgat ggcagatata ttgaaagttc agaagaggaa ataaatagtt
     3421 attttgaaga aaactgtatg ataaactcta cagctgatgt agagcacaat gatggtgata
     3481 ttaagtattt aggaagtgct actgaatgtg cattgctttt atattacaaa aacgtagatt
     3541 atagacaagt tagaaaaaat tctaatatag tagctcaaaa tccttttact tcagattcaa
     3601 agagaatgac ttctgttata ggtcaagata atcatcatgt actattatca aaaggagcac
     3661 cagaagtctt acttgaatta tgttcacata ttcaaagagg aaaagacata gttccattaa
     3721 ctgaaaatat aaaacatgag attttagaag aaataaagaa attgcaaata aaatcaatga
     3781 gaactttagg gtttgcatat aaagaaatct cacaagctga agaagaagca gcagtaactg
     3841 ctgaaagtga tgctatgaat gtaagtgcaa tggaaaacaa tttagtattt agtggttttg
     3901 ttggtataag agacccactt agaaaagatg ttatagagtc tgtaaataca gcaaataaag
     3961 ctggtgtttc tgtaaaaatg cttacaggag ataatataaa tacggcaaga gctataggtg
     4021 aagagttagg cctattaaaa aataatatga gagtggttga agcttcatat atagatactt
     4081 tagatgatga agaattaaaa caagagatac aaagtatttc aatcgtggca agaagtaaac
     4141 cagatagtaa aatgagaata gtttcagctc tgcaaaagag cggagatgtt gttgcagtaa
     4201 caggtgatgg tataaatgat gctccagcac tttcaaaagc agatgttggt atagcaatgg
     4261 gtatatctgg tacagaggta tctaaaaacg cagcagacat aattttaact gatgatagtt
     4321 ttagtaccat tgtaaaaggt attaaatggg gtagaggaat ttatgataac ttccaaagat
     4381 tcgttcaatt ccaattaact gtaaatgtaa ttgcattttt agtggctata atatctcaag
     4441 ttatgggaca agagatgcca tttactacaa tacaattatt atgggtaaac atcattatgg
     4501 atggaccacc agcactagca ttaggtttag agccagttag ggaccatgtg ttaaatagaa
     4561 aacctgttga tagaaaagcc aatataattt ctaagtcaat ggtgtataca atagtattaa
     4621 atgcatttta tataacagca atactaatgt tacaatctac atttaatttc ttaggagttg
     4681 atgaagcgca taagggaaca gtaatgtttg cgttatttgc ttttagtgca ctgtttaatg
     4741 catttaactg tagagagttt aatgcagata gtatcttccc taattttaca cataacaaat
     4801 tagcactaca aataatagtg ttgacaggaa ttgcacaagt aatatttact cagctattcc
     4861 aagatttctt taactctgta gcaatggatt ttatgacatg ggtaaaagtt ttatgtgtag
     4921 cagcaagtgt aattgtggtt aatgaagttg taaaatgtat aataagactg gtaaaactaa
     4981 aaaagactgc ttaaaacaaa ttttttagaa gaaaacagac tgactcaaaa tatgaatttt
     5041 gggtcagttc tgaataaaca tttaaattgg gaggatagtt atgggtgact caaaggtatt
     5101 tgaaaaaata ttctccagcc aatcagatag aggaaattac actccttcaa agggatatct
     5161 aagttatttt atttcgtata ttggtttaga agatgaagtt ttgtacaatc tcgagatatt
     5221 taagacaaaa caaaatattg atagcaaaaa agatattgct ttatttactg atgttatagc
     5281 aaatcctagt gattttgata ttataaatta ttttaaaagt ggacttcaaa aatatagaac
     5341 tagcatggaa gatgtagata taaatatttt gggatttgaa gagattgatt ataaaataaa
     5401 gcaagcaatg gatagagtgt taaaagaaga ggaaaaagaa tttactaatg atagagttaa
     5461 acaaaatttt atagtgaaaa taatggcatg gataaaaatt tatatcggtg ctttagatat
     5521 aaataaaaat gaagctccaa aagtaatatt ttatggagat ataaaaaaac atgaagttta
     5581 tttactctta atattatact tggcaggttt tgatgtttta tatcttaatc caaattcaaa
     5641 gagtaatata gatattttaa aatcagaaag atacaatata gagtttgaag aagccaacat
     5701 aatagaagaa aaaatttctt ttgaagaaag ggttatttta ggggaaaaaa tagataaaag
     5761 ttctgtaaaa aaagctttta ctgttggggc agaagcatca aagcgtataa gtgaggaatt
     5821 gttaaatgat gcaggattta taaaaccttg gcaactccaa gatagaaaaa taaaaaatct
     5881 acttctaagt tcaactgttg atgaaatatc aatatactgg aatcaaccac ttaaattaag
     5941 accaggattt aagtttaatg atgctattgt agaagctcca aattttcttt ctaagattaa
     6001 tggaatatat aacgataaga atgaatatat taagttttta gatttattaa gagattcgga
     6061 aagttcaaca tttattgaat ttaatggaga tgtagataga ttttcaaaag cttttacaag
     6121 agaagctttt agtttatcct ttttattgga ttcaaaaggt gcaatagata aaaactctgt
     6181 tttaaataat aaagattatt ccatatcaac actagcattg aatcaacaaa ttatgatttt
     6241 agagaaagtg gaagagttgt tagaaggaag tatgtttttg aatggtttaa gtggagaaga
     6301 caagataaaa ggtctattta cagtacttca catggataag aaatttgttc atatgatgaa
     6361 caatttcgat tactcactta taaacccaaa attgattata tacatgtaca agagtattgt
     6421 atttgataaa gaaatagttt ttcttatgct tttacttagt aagattggat ttgatattat
     6481 catattatgt ccaggaggag aaaacaatat agagaatgtt ataaataacc aattgattga
     6541 tgtacacaga ttggataaaa tggtatatga ccttaaatta aattcgcttg aaaatgatat
     6601 acctttactt aaaaaaattt ttggaaagag gagacgattt tagatgggga taaatataaa
     6661 tagtttttca aaagataaga actctgtaga ggttactgaa aatttgccta cagcagttca
     6721 agaagaaaac ttcgatataa tggaatatac aaataataaa aaaaatgaac tgagaaagtc
     6781 taaggaagtt gaggcattga catctcttat agaagtagaa aatccagata cgattttaca
     6841 gtttggtagg aaagcatctg aaggagtagc aagagtttct gatagccttt taaatacaat
     6901 aaaattaaat agaaatgaag aaaattctaa aatgttggtt catttaacta aaataatgga
     6961 caagtttgat ttagacgatt ttcaagaaac taaggaacca aatttcgttc aaaaactttt
     7021 caaaaaagct aataatgcca ttgaaatgat gtttcaaaag tatgaaacat tgggaggaga
     7081 agttgagaaa attcaaattg agcttgaaca atacgaaaga gatatagcac tttctaataa
     7141 gcaaataggt gctatgttaa atgaaaactt tgagttttac aatgaacttc aaaaatatat
     7201 tgttgctgga gaaatggcaa tagaagaaat ggataatgaa atactacctt tctttaaaca
     7261 aaaatcagag actagtggag accaaatgga tgttgtcaat tatcaagaac tattaaaagt
     7321 ttatgatatg ttaaatcaaa gagtctatga tttaaggata gcagaaaata tagctatcca
     7381 aacaatacct atgcttagag gtatgcaaca taataattat ggtttaatta gaaaaataaa
     7441 tagtgcattt gtcgtaactt taccagtatt taaacaatgt ttatctcaag ctatattact
     7501 taaaaagcaa gaattacaag caaaatctct aaaagcactt gatgacaaaa ctaatgaatt
     7561 attgcttaga aatgcacaaa atgtaagtac tcaaagtgca caaatagcta gaatggctgg
     7621 aacaagttct gttcaaatag aaacattgga aaaaatgtac aatactatca agtctggtat
     7681 agatgaaact atgagaatag aagaaaacaa tagagcttta attaaggata atacaaagag
     7741 attggaagaa ttaaatacga ctataattta taataagtag tattatatta agggctaatt
     7801 tgtataaagt tggccctttc tatgtatatg aggaggctag acattgaaat atttcgatta
     7861 tatctgcaaa gatgatttgg agagaatttt tttgaaggag ccagaagatt ttagtgctaa
     7921 gactgaaaaa gatgtgttga aatatgcact gggcgctttt ttatacgttc cagctactca
     7981 atataatatg atttataaga gtattatagg ggatgttaag ggagttagac cattagctat
     8041 ttgtttagaa gatgctgttg gagtaaatgg agagttggag gctatagaaa atcttagatt
     8101 aattttaaaa aatattagca atgaaagtat aactaataaa gatggaatac cactaatttt
     8161 tgtaagaata aaagatgtgg agcaactgtt gagaattaaa gagattataa ttaaaaatag
     8221 tcattttata acaggaatat taattcctaa agcaaattcg gagttaattg aaaattgtat
     8281 agaagcttta gattttatga atcttcaaga tatgtatgtt attccgatag tagagacaaa
     8341 agaatttata tataatgaaa aaaaagaact tagttttact aatttgtaca atgctatttt
     8401 aagacataag tcaagaatat taagcattag agtaggcata actgatatat tagggatgta
     8461 tggtataagg agagataaaa acttttctat atataataat ttgatttgtt cttcatttat
     8521 attagatata atcacatatc ttcaaagacc agagttagat atacctataa gtggtggagt
     8581 atcagaattt tttgatatga cgaataagga tataagaaat aagtatatag aagaaatatt
     8641 attggataaa tatcatggat taattggaaa gaccgtaatt catccaatgc aaattcaaat
     8701 tgtacaagcg ctatctacag tatcatatga agattttact gatgctttgg atatactgga
     8761 tagtacagac agtaagtatg gtgtgagcaa gggtgtatta ggagagagaa tgaatgaaac
     8821 aaatccacat tttttatggg ctaaaaaaac attgattcta tctaaaatat atggtgtatt
     8881 aaataaagga gtagactatg aagaattgct taaattttaa ttgtgagatt gacattattg
     8941 ataatccatt gaatttagaa ctaaatgatt ttttagacgt aggaataaga aataatagca
     9001 aaagacgttt tttgtttatc tcaaagaaac tcggtaaaca tttagcatgt aagccaagtg
     9061 agatggataa tttgggaaaa ttaatggcta caatttataa tgaaaaaaat aaggaagaat
     9121 caaatggtac tgttatagct tttgctgaaa ctggtacagc tgttgggcat agtttttttg
     9181 attacttatg tggagattat gaatttattc atactactag agagcagttt gacgagttag
     9241 aaaatctgga atttttagag gaacattccc atgcaacaga ccagaattta tactttggta
     9301 tgcttaacaa ctttaaattt ggagatgaga taatattggt tgatgatgag attaccacag
     9361 ccaatacatg catgaatata attaggaaaa ttcaaagcat ttatcctaaa aagaaataca
     9421 ctatatgctc aatactcaat tgggtaagtg atgaaaattt aagtaaagtt actcgtttag
     9481 aaagagagct agattgtaag atagattttg tatacttatt taaaggaaac tttaaatttt
     9541 atatagatga agatattatt tcaaattaca acgataatta tagtattact aaattagaga
     9601 ctatttcaag tgataaagta aatttagtag taaatcatat ttatgttgat ttagaagatt
     9661 atattggaaa taagaaatat gtaaaatata caggaagatt tggaattaat agaagagaac
     9721 aatttagatt aaaagaaatc gttaagagag aaagtaaaaa aataactatg gccagtaaag
     9781 aatctttaaa aaatgaaaaa gaaaagattc tatttttagg tactgaagaa tttatgtata
     9841 tacctatgct gtttgcaaaa gaaaaagaaa atcaatatga tatatactat cattcaacaa
     9901 caagaagtcc aattattaat attgataaga ataattatcc tattaaaagt aaatttatgc
     9961 taaagagttt ttataatata gatattcaaa attatattta caatatagat aaacataatt
    10021 tttctaagtg ttatttattt gtagagctga ataaaagtga aaattcttat ctagaattta
    10081 ttgatataat taaaaaaaca agtataaaag aattaactat agtatgttgt agctaaagtg
    10141 ttttaacagg ataaaatata tcacgatttt aagataggag ttgagtcata tgagagattt
    10201 tagcacgtat tctaaagatg atgttatttt tttattaaaa gatatatcaa atttaattga
    10261 agaagaagga aataaagaaa gagaattaaa aatacaaaat ggtagacatt attcagaaat
    10321 gataccaata gagtatgaag ttagtgaaga ttatctgaat ttatatcatg agaaactaag
    10381 agaaaataaa gataaacttg cattttctat aggagttatg tgtgaaaaaa taattaaaaa
    10441 aaatggaaat gatgtagtac tagtttcact tgctagagca ggaacaccta ttggtatttt
    10501 ggcaaaaaga tatataagaa gcaagtataa tataagttta cctcactata caatctctat
    10561 aataagagat aagggaatag atatgaatgc aattgaatat atagtaaaga atcatccgag
    10621 ctcaaaaatt caatttttag atggatggac tggtaaggga actatatcaa aagaacttga
    10681 aaaagcatgt gatgaactta atgttaaatt taaaaaatat tttgactcaa cacttgctgt
    10741 attagctgac cctgctggat attcaggact ttatggaatt agagaagatt tcttaatacc
    10801 aagtgcatgt ttaaattcaa cagtttctgg tttagtaagt agaactgtac taagagagga
    10861 tttaattggt aaaaatgact tccatggagc taagttttat agacatttaa aagataaaga
    10921 tgaatctatg aactatatcg aaactataga agaatgtttt aaaaatcaat ttaagaatat
    10981 atctgatgaa gttgaaaact gggaaagtga cattataaca agagatggat actttgatgt
    11041 tttaaatata aaagaaaagt ataacataac agatattaac tttatcaaac caggtgtagg
    11101 ggaaacgaca agggttttac ttagaagagt tccgtataag attcttgtta aagacttaaa
    11161 tgataaatct ttagaccata tattcatatt agctaaggag aaaaatgttg aagttgaaca
    11221 aatggattta aaagcctata aatgttgtgg aataataaaa aatatgaaag atatttagga
    11281 aatatattag acaggagaat attgaatgat tgttttttca gatttagaca ggagtatcat
    11341 ttactccaat aaatttttaa atgctgatag taaatatact aatatag
//
LOCUS       DAYASV010000119        11234 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYASV010000119 DAYASV010000000
VERSION     DAYASV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11234
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.121"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            156..923
                     /locus_tag="ACGI68_002972"
     CDS             156..923
                     /locus_tag="ACGI68_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HFH0139398.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPYF
                     KNCVKKRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1141..1354
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1608..2642
                     /gene="argC"
                     /locus_tag="ACGI68_002973"
     CDS             1608..2642
                     /gene="argC"
                     /locus_tag="ACGI68_002973"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFH0139399.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKVASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2683..3900
                     /gene="argJ"
                     /locus_tag="ACGI68_002974"
     CDS             2683..3900
                     /gene="argJ"
                     /locus_tag="ACGI68_002974"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFH0139400.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3915..4775
                     /gene="argB"
                     /locus_tag="ACGI68_002975"
     CDS             3915..4775
                     /gene="argB"
                     /locus_tag="ACGI68_002975"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFH0139401.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKETE"
     gene            4805..5992
                     /locus_tag="ACGI68_002976"
     CDS             4805..5992
                     /locus_tag="ACGI68_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HFH0139402.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVILKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6101..7123
                     /gene="argF"
                     /locus_tag="ACGI68_002977"
     CDS             6101..7123
                     /gene="argF"
                     /locus_tag="ACGI68_002977"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFH0139403.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7250..7867
                     /locus_tag="ACGI68_002978"
     CDS             7250..7867
                     /locus_tag="ACGI68_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HFH0139404.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8087..8785)
                     /locus_tag="ACGI68_002979"
     CDS             complement(8087..8785)
                     /locus_tag="ACGI68_002979"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFH0139405.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHDLMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8947..10164)
                     /locus_tag="ACGI68_002980"
     CDS             complement(8947..10164)
                     /locus_tag="ACGI68_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HFH0139406.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTTDLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10355..10768)
                     /locus_tag="ACGI68_002981"
     CDS             complement(10355..10768)
                     /locus_tag="ACGI68_002981"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139407.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYYRKIL"
BASE COUNT         4432 a         1209 c         1840 g         3753 t
ORIGIN      
        1 aattatatat acaatttaat aattcagata atcttaaaat aatattatgt aaaataaaaa
       61 tatatatgac atgatgttgt tgtattcaaa atattaagat aaaaatatat agaatatatt
      121 taaaaattat tatgcaatta gaaaaggaga taagtatgca gattattgat ataaatataa
      181 ataatattga caatgaacat atatgttgtt caatttctga aaaaaaagga gaaaattgtg
      241 ttttttctaa aaaagcttgg atgaaaaata attttgataa aggtctagtc tttaaacgat
      301 ttgatgtaag aggtaaagct tttatagaat atattccagc agaaaatgca tggtgtccta
      361 ttactgcgga caattatata tttattaact gtttttggat atctggaaaa tttaagggtc
      421 aaggatattc taatctattg ctacaagagt gtgttgatga tgcaaaatca aaaggtaaat
      481 atggattagt tgtgctttcg tctagtaaaa aaatgccttt tttatctgat gctaaatatc
      541 taaaatataa ggacttttgt ttagcagata catcagaacc aaattatgag ctattatatt
      601 ttccttttac tgataatgct cctattccat attttaagaa ttgtgtaaaa aaaagaaaat
      661 taaatgagaa cggtatagtt ttatattaca gtaatcagtg tccacatacg gaaaaatatg
      721 ctcctatttt gaaaaaaatt gcaaattcta gaggtattga tgtaaatctt ataaaattcg
      781 aaacagcaaa agaagcacaa aatgctcctg caccatttac tacatacagt ctttttttta
      841 atggtgaatt tgtaaccaac gagatacttt ctgagaaaaa gattgagaaa tttttagata
      901 ccaacattaa aaaatttaac taaaaagaga tgtaaatatt gtattttgaa aatatattat
      961 tttataattt aatatatttt ggatgttttt taaattgacg aatgaaatat aaaaattata
     1021 tcgcaattta gatacaaaat gctggaaatt aaccatatag aaaatatttc caaaaattta
     1081 aaaattatat tgacaaagtt aaaaaaataa actatcatat aaaacaataa gaaaataaat
     1141 tgaaatcctg tgaaaaagag agtagcataa agctagttga tagagagctg ggaatggtgg
     1201 aagcctagta acgaatttta ttgtgaatag agctttggag gagattacca gaagtaatag
     1261 tatggtaatt ggtgaatctc gccttaagag attagagtgg gtaagattat cttatcaatt
     1321 agagtggtaa cgcgggtgta tattcgtctc ttggtctttg accaagaggc ttttttaatt
     1381 ttaaatgtac attttgtaga tactttttaa tttgagtttt aatgattatt gtaattttac
     1441 caataagagt ggtaccgcgg aaaatcgcct cttagtattt ataatgctaa ggggtttttt
     1501 gttttaatta gagtatttta atctacgtat tatcaaatga ttatcatata gtaaagtgga
     1561 aagataggat tttaagacta tttgaatata aaaatggagg gaacaaaatg aataaaataa
     1621 aagttggtat tattggtgca actggatatg tcggggcaga gttgattaga ttattaatga
     1681 atcatgataa agtcgaagtg acagcgatag gttcaaattc gtatgttgga aaagatatag
     1741 ttgatatata tccaagtata ggatataaaa ataacatgat atgtatagaa aatgaaaaag
     1801 ttatagatat gtgtgatgtt gtatttacag cccttccaca tggagttagt gaaaagtttg
     1861 taataaaggc aattaaatcg aaaaagaaag ttatagattt aggtgctgac tttagaataa
     1921 aagatgaaga agtatatagt aaatggtatg gagtatcttt tatagataaa atcttacata
     1981 aaaaagctgt atatggatta agtgaaatat acaaagaaga tataaaagat gcagatataa
     2041 ttgcaaatcc tggatgttat ccaactagta tttctttacc attgatgcca ttactaagtt
     2101 ctaaattgat aaaaaataat aatataataa ttgattctaa atcaggtctt acaggtgctg
     2161 gtagagaatt atcgattagt agtcacttta cggaggttaa tgaaaatatc actgcatata
     2221 aaatagggaa gcacagacat actccagaaa tagaacaaaa tttaagtgaa tcctgcaaag
     2281 aaaaagtaag tgtagttttt acaccaaact taatcccagt aaatagggga atcttatcaa
     2341 ctatatattg tacaaaagaa gataatattt ctataaatga tatacatagg aaattaactg
     2401 attattatga atttaaagaa tttatagaaa ttcttccatt agataaggta gcttctttaa
     2461 agaatgttag attctcaaat aaatgtgtaa tatcattaca tgaaaatgga gatacattga
     2521 taatctgttc agctatagat aatatgatta agggagcagc aggtcaagct atacaaaaca
     2581 tgaatataat atttggtatt gaagaaaata caggtttaaa aaatattgca ccatcatttt
     2641 agaaataaaa aatactaaac tcatataatt aggaggataa atatggagat tataaagggt
     2701 ggagttacag tttcagaagg attttttgct tcagggattc actgtggctt gagaaaaaat
     2761 aaagaaaaaa gagatttagc tttggtttat tcagatgttt tatgtgatgc agcagcagta
     2821 gtaactcaaa ataaagtaaa gggcaatcct gtttatgtta cacaggaaca cattaaaaat
     2881 ggaaaagcac aagctatcat agtaaatagt gctaatgcta atacctttaa tggaaaagaa
     2941 ggacttataa atgcttataa aatggctaaa ttcaccagtg ataaattaaa tattaaagaa
     3001 agtgatgttt tggttgcatc tacaggtgtt ataggaaagc cattaaatat agatttaata
     3061 gaagaaaata tggatgaatt agtgaataat ctaagtaaac aaggtcatat tggagcaaga
     3121 gaagctatta tgacaactga tattataaaa aaagaaatag cagtagcaat aatgtatgga
     3181 gataaaaaaa taactattgg aggtatggca aaaggttctg gcatgataca tcctaatatg
     3241 gcaacaacgt taggatttat aactacagat gcaaatattg atggagtttt gctaaaagaa
     3301 gctttaaaaa ttgctgttga caaaagtttt aatagagtta gtgtagatgg agatacaagt
     3361 actaatgata tggtaatgat tctagcaaat ggaaaagcaa aaaatgatag aataaacaaa
     3421 aaagatgaac attatcaagt atttttgagt gcacttacat atgtctgtat agaacttgct
     3481 aaacttgttg caaaagatgg agaaggatgt acaaaactaa ttgaatgcta tataaatgga
     3541 gcattaagtg aagaacatgc agtaaaatta gcaaaaacag ttatatcttc ttcattagta
     3601 aagacagctg tatttggagc tgacgcaaat tggggtagaa tactgtgtgc tttaggttat
     3661 gcaggtgaag aaatagatat ggaaaaagtt gatattattt ttgagagtat gaaaggatat
     3721 attgaagtat gtaaaaatgg aaatggatta gattttaatg aggaaaaagc aaaaaaaatc
     3781 cttgaagatg acgaaataag tatattagta gatttaaata tgggcaatgc cagaggaaat
     3841 gcatggggat gtgatttaag ctatgattat gtaagaataa atggaagtta tagaaattag
     3901 gaggatatac tttaatgatt aatatagaaa aagcaaatac acttatagaa gctttaccat
     3961 atatagaaaa acatcaggga aagactattg tagttaagta tggtggaagt gcaatgaaaa
     4021 aagatggtct aaaagaatct gttatggaag accttgtgtt aatgagttat gttggaatca
     4081 atatagtttt agtacatggt ggaggggcag aaatcaataa gatgctagca aaagtggata
     4141 tagaaagtaa atttgtaaat ggtcttagat atacagatga agagaccatg gaaattgtaa
     4201 aaatggtact tgctggaaag gttaataaag acttagtaaa taaaatacat acaaaaggtg
     4261 gaaaagcagt agggctttgt ggaatagata ataatatgat tttatgtgac ccatataaaa
     4321 attatgaact tggatttgta ggagaaatca aaaaagtaaa tgttgagctt atagaatctt
     4381 gtctaaagtc aggttatatt tcagtaattg caactatagg tgttggagat gatggagaga
     4441 cttataatat aaatggtgac acagcagctt ctgctatagc taaagagtta aatgcagata
     4501 aattgatact tcttacagat gtccctggtt tgttaagaga gccagatgaa gaaaaatcat
     4561 taataacaga agttatatta gaggatgttg ataaattatt tgaagaaggt ataattactg
     4621 gaggtatgat acctaaaata gagggatgcg tagatgcctt aaataacgga gtaaataggg
     4681 tacatatttt ggatggaaga gttccacatt ctataataac agagctattt actgatagtg
     4741 gaataggtac attaataaga aaagaaactg aataaaatta taagtttaag aggggagaat
     4801 aagaatgaat aacgacaata caatttcaaa atggaatgaa tattttatag atacatataa
     4861 ccaacccaat tttgtcatag attatggtga aggaagttgt ttttttgata ctaatggcaa
     4921 taaatatata gattttacaa gtggatatgg agtatcttct cttgggtata gtaatagtaa
     4981 tttaaagaat gctctaaaag agcaagtaga taaattatta catacttcaa atttatattt
     5041 taatgagcca gtgttgtatt caggagagaa aataattaat tcaagtggaa tggctaaagt
     5101 atatttttgt aattctggaa cagaagcaaa tgaaacagca ttcaaaatag caagaaagta
     5161 tagtagtgac aaatatggta atggtagagg aactatcata agtttaaaag actcttttca
     5221 tggtagaaca atgatgtctt taatggcaac tggtatggac aaataccata agtattttta
     5281 tcctcttccc gaaggtttta aatatataga aagaaataat attgaagatt taaaaaataa
     5341 tcttgattcc acagtttgtg caataattct tgaagcgatt caaggcgaag gtggagttaa
     5401 tgttcttgaa aaagattatg ttttggaaat agtaaaaata tgtcaagaaa aggatattgt
     5461 agtgattttt gatgaagtcc aatgcggtat aggaagaact ggtaaacttt ttggctatga
     5521 atattttgat gtaaaaccag atatagttac cgtagcaaaa gggttgggag caggaatacc
     5581 agtaggagga gtattggtaa ataaaaaact ttcaaaagta ctaggaaaag gagaccaagg
     5641 tacaactttt ggaggtaatt tattagctat ggtggcagct tcagttgtat tagatgagat
     5701 atctaaagat ggcttttata atgaagtatt agaaaaagga aattatataa gaaaatctat
     5761 tgaaagtttt aataacaaag ttatattaaa gacaaaaggt attggtctaa tgataggaat
     5821 tgaaactaat attgaatcta gtattataga agagaaagct agaaaaaaag gattgcttat
     5881 tttgactgct ggaaaaaatg tattgagatt tttaccacca ttaacaatca gttataagga
     5941 aatagatgag gctcttgaaa tcctaaaaga tatattacta gaaattaact aaatattcaa
     6001 aaaatagttg attaaagata aaaattagaa gtaaataaaa aagtaaagtg ctaacaagtt
     6061 taattgaatt taaaaagttt ataagaaggg gcgaattaat atgaataata taagtttaaa
     6121 aggtaagagt tttttgacat taaaggattt tacaaaagaa gagattagat atttattaac
     6181 tttatcaagt tatttaaaaa ctaagaaaaa agttggtata aaaggcgatt tgctacaagg
     6241 aaaaaatata gctttattat ttgagaaaac atcaacaaga actagatgtt catttgaagt
     6301 agcagctcat gatgaaggag cacatgtaac ttatctaggt ccaaatgatt gtcatatggg
     6361 taaaaaagag tctgtatcag atacagcaaa agttctagga agattctatg atggaataga
     6421 atttagaggc tttaaacaag aaacagttga aagtctagca aaatattctg gtgtacctgt
     6481 ttggaatgga cttacagatg aatatcatcc aacacaaata ctggcagatt ttttaacagt
     6541 aattgaaaat gtagataaag atttgaataa agttaaattt gtatatgttg gtgatgctag
     6601 aaataatatg gggaactcac tgatgatagg atgtgctaaa atgggaatac atttcgtagc
     6661 actagcacct aaggaacttt ggccaaatga agaattggtt aaggaaatga aggaaatagc
     6721 caagaaatcc aaaggagata taatgttgac tgaaaatatt gatgatgtta aaggtgcaga
     6781 tgttatatat acagatgtat gggtttctat gggagaagag gagcaatatg aagcaagaat
     6841 aaatcaactt aaagattatc aagtaaatat ggatatgata aagaaaactg aaaatgaaaa
     6901 tgttatattt ttacactgtc ttccagcatt tcatgattta gaaactaagg ttgctcagga
     6961 gatttatgaa aagttcggat tatcagaatt agaggttaca aacgaggtat ttaattcaaa
     7021 atattcaaag gtatttgaag aagctgaaaa tagaatgcat acaataaaag ctgttatggt
     7081 ttctactatg actgatgtaa atatgaacaa taatttaata tagatatgtt ttaatataga
     7141 ctttacttta atgaaaattt ataaaatatt ttaaatatat tattcaaaaa ctctcattat
     7201 atgttaaaat aacttatgta tcaacaaatt aagagggagg atttgagata tgaaaaaaat
     7261 catattagct ttaacaactt ttttattagc aatatcttta acagcatgtt caagtgcaaa
     7321 accagaagat acaattgata gtttttttaa cagtgcgaaa aagtttgact ttgaaggcat
     7381 gaataaggta atggaaaata atgacgaaaa atataaggat atattaaagg aattagatac
     7441 aaaagaccca aatgcccaat atgttttaga ttatctaaaa cagaatgcat ctaaaatcac
     7501 atatacaata aaagacagcg aggttaaagg tgataaggct acaatcaaag tagaatgtaa
     7561 atttatagat tcaacaccat tacttcaaga aatagtagct gaggcattta caaagatgat
     7621 tggtatgagt ttttcaggac aagacttaac tgatgagaaa acaactgaaa tgttagtatc
     7681 aattatgaaa gaaaaacaaa aaagcgttaa agagacttat gttacaaaaa atgtagagtt
     7741 tgaatgttct aaaaaggata ataaatggat aataagttca gtaaatgatg cagtagctga
     7801 tgtattgtta tctaatcttg ttacagcagg acaagaattt tctaactcta tgaatacact
     7861 tgaataatat tgtttaggtg tagtaaagta ataaatatat gattattttt ataaataaac
     7921 atataaatat ttatgaagga taccattttt ggtatccttt ttattaatat aatgaaatac
     7981 atggttaatt tctatattgt caagaaacta tgatatatgt tataagtcta tactatttaa
     8041 aataaactta taacatatat tttttataat atagtgtatg aaaaatttat ttaactaatt
     8101 ttagatattt attgacaact gattcagcca agcattttga tgaatcaaca atatttattg
     8161 aaggatatga tttttctaaa actacattta aatcagtaca agcaattact atattttcta
     8221 tagattcatt tttaacatct tcaataagtt tattccaaat atcaatattg tgtggatttt
     8281 ctttatccat ttttatattt tgaataagat tgtttaattt tatttgccat tcatctttaa
     8341 atataaattc atgtccatta tgtattatac cattctggta aattgtagac tcaaaagttg
     8401 aactagtcga aaaaagagtt actttttgtg agatagtagg taatttttta actgtttctt
     8461 ctacaatatt taatattggt atattaatag actcttttaa ttctttaaag tatatatgtg
     8521 ctgaattgca tggcattgca ataaaattta caccagttga ttcaagcttt tgtaatccat
     8581 caattatagt ttctttcata aggtcatgat ttattggacg gtcaatataa aatggggtag
     8641 gtaatgaata tatcatcatc tttggaaatt catcatcata ttttgctcca tattgagatt
     8701 gacactcatc aataattaaa tcaacaaaag gggctgttga ccttggaccc attccagcca
     8761 aaataccaat taatttttca ttcataaaaa ttatctcctt tatatataaa tatatttatg
     8821 tataaaataa aaatctataa aagcataaaa ttatatttta taatagatta tattgtacac
     8881 ttatataata taaatataaa gtgatttttt agattatata ttttaatata ctttcttata
     8941 taagaattat tttaaatcaa gattatctag tatttttaaa aatattttta cagcagtatc
     9001 caagtcatct acagagacac tttcattagg attatgactt acgcccccag cgcatcttat
     9061 aaataccata ccaatatcag ttatattatc catttcctga gcatcatggc cagcaccact
     9121 ataaatataa aaaggattaa gatttaaatc tataaaactt ttttctatga tttttgttat
     9181 tttattagaa catggtacag gaacattttc aaaagcaagt tcacttgtat aattaagttt
     9241 tctattttca catatatgtg atatttcatt aaatatcttt tctacagaat tatcaagaat
     9301 ttcttgtgaa caagagcgta catcgagtgt aaattcagct ctttcaggta ccacatttga
     9361 tgaagatgga tagaaattca tctttcctac agtagcaacc aaatcagtag tcgttttagc
     9421 tactttttcg atagctaata cacattcaca ggcacaacat ccagcatcaa gtctcatatt
     9481 cattggaagt gtaccagcat gtccagactt accatttact gatatcttaa aacttttaaa
     9541 tcctgtaata cttgatacaa ttccagcagg aagagattca ttttctaaaa caggaccctg
     9601 ctcaatatga aattctaaat atgcatctac atcttttcta ggatgcaaat ttgttaagtt
     9661 ttttgtatta aatccaaact ttgttacagc ttcttcaagt gtaattccat tttcatcaac
     9721 agatttaaga agttcctctt taaaagtacc agcaaaaact ttacttccca ttaaagaagt
     9781 ttcaaatcta gttccttcct cttcagcgaa agcaattagc ttaatattga aaggataaga
     9841 cctattattg tgttttgctt cttcaagacc aactagaggc actattatac ctaacatacc
     9901 atcaaatata cctccacact ttacactatc ttgatgagat gcaactacta atgtgggagc
     9961 atttggaaag cttgatttat atgtacctat aacgtttcca atgtcatcaa tttcaatatc
    10021 aagatttaaa ccttccatcc atgaactaat taaatcaata gattttttat gttcttgagt
    10081 caagataagt cttgtaagac catttgttgt tgaacttatt tttcctaatg tttgtagata
    10141 atccatacat ttttttccta acatatttac ctcctaaaaa tataattttt atttaagcta
    10201 agattagctt ttatacttat aatttttgaa ctttcaaata attttgtatg tatagtaaaa
    10261 tactatttta gtattaataa tgatataaaa acataaagtc tgtagacgaa gagcagaagt
    10321 tttaatttaa aaatatgaaa tatgattttt attattatag tatttttcta taataatatc
    10381 tctttccagt tagaggataa ttttcaagtg aaaatacttt atggtatcca aatttctcat
    10441 aaaatttagg tgcttgaaaa ctgaatgtat ctaaaaaaac atatttacaa cctcgttctt
    10501 tagcaatttt ttcagcctta tataaaagtt gacttccagt acctttatat ctaagcttct
    10561 cactaaccca taagtatttt atagaaagcc aattaccatg agtaacacct ataaaacctg
    10621 caacaatttt acctgcttta tctcgaatat atattcctaa atccactggg tttttatctt
    10681 caagtctagc aagattatat tttaataatc cttgaaaaat agtatcttca tcttgttttt
    10741 ctattttttc tgtaatttct aattccatat ttgaactttc tccttgtata atcttttgta
    10801 tattggtatt ttttaataaa atcagtagaa agaatatagt ttaacattat tctaataggc
    10861 atttatgcta atgtcaaact aataatactt attcttaatt ttatattgtt gatattaaat
    10921 ttttttaaat aaaaatgtag tttaagttat ataaaaagaa atcatcaata aatttagtaa
    10981 acctaaattg ttgaatatta aaataagtta atttaataca taaaaactat tttagatgtt
    11041 attctattgc tatgttcaat aatatcatta gtagttcaat ttataaaatg aataaattat
    11101 ataaataaga ttagaaagta gtaaatagtc ttattaaata taagtaataa aataataaat
    11161 acctagtaga gattaataat taaaaaataa aatactacca aaataaactc gatagtattt
    11221 tatttaaata ctta
//
LOCUS       DAYASV010000120        11159 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYASV010000120 DAYASV010000000
VERSION     DAYASV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.122"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..676
                     /locus_tag="ACGI68_002982"
     CDS             <1..676
                     /locus_tag="ACGI68_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139408.1"
                     /translation="FLINPEDAFDYIIFYIFILLIIYVAIAAPTFSGEYQTQSDSILR
                     CTKHGHICLAFTKILALFSIFIIMFVICISIYLAISDLAFGTECLKTSFQMLFSVISL
                     VNINLLQAQILIAIGGLISILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSW
                     IGYLLPPGGIGFKISLLYQMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIHTY
                     CKHQVS"
     gene            1057..1974
                     /gene="pstC"
                     /locus_tag="ACGI68_002983"
     CDS             1057..1974
                     /gene="pstC"
                     /locus_tag="ACGI68_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFH0139409.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1976..2800
                     /gene="pstA"
                     /locus_tag="ACGI68_002984"
     CDS             1976..2800
                     /gene="pstA"
                     /locus_tag="ACGI68_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFH0139410.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2819..3583
                     /gene="pstB"
                     /locus_tag="ACGI68_002985"
     CDS             2819..3583
                     /gene="pstB"
                     /locus_tag="ACGI68_002985"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFH0139411.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3611..4264
                     /gene="phoU"
                     /locus_tag="ACGI68_002986"
     CDS             3611..4264
                     /gene="phoU"
                     /locus_tag="ACGI68_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFH0139412.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4422..5291
                     /locus_tag="ACGI68_002987"
     CDS             4422..5291
                     /locus_tag="ACGI68_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFH0139413.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5378..6814
                     /locus_tag="ACGI68_002988"
     CDS             5378..6814
                     /locus_tag="ACGI68_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HFH0139414.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     NLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6862..7689
                     /locus_tag="ACGI68_002989"
     CDS             6862..7689
                     /locus_tag="ACGI68_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0139415.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    7985..8247
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8323..10989
                     /locus_tag="ACGI68_002990"
     CDS             8323..10989
                     /locus_tag="ACGI68_002990"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFH0139416.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4408 a         1196 c         2081 g         3474 t
ORIGIN      
        1 tttcttaata aatccagaag atgctttcga ttatataata ttttatattt ttatattatt
       61 aattatatat gttgcaattg ctgcacctac tttttctggt gagtatcaga cacaatcaga
      121 tagcatcctt cgttgtacga aacatggaca tatttgtcta gcgtttacta aaatattagc
      181 tctattttct atatttataa taatgtttgt tatatgtatt tctatctatt tggcaatttc
      241 tgatttagct tttggtactg aatgtttgaa aacctcattt caaatgttgt tttctgtcat
      301 tagtctagta aatataaatt tactacaagc acagattctt atagctattg gtggtctaat
      361 ttcaatactt gcaatggtta gtttaacatt atttttatct gcaaaatgta aggattctct
      421 tacttctatg ctgattgctt tttctatttg cttaattcct atgggaatag gtgttagttg
      481 gattggatac ctattaccgc cagggggaat tggttttaag attagtttat tatatcaaat
      541 gacatcattt aatttcttac acttaggagg tatgagtttt tggacaccac acattatact
      601 gtttttctca gtgatagaaa tacctatttt tatatttttg gctattcata cctattgcaa
      661 acatcaagta tcgtaacaat ttgatggaaa taatagtgat ataaactaga ggatacaata
      721 attatatatg tagtataaaa gttaaattta gttaacaaat cttaatagca gatataggta
      781 ataaattata cataaactgt attattatct atatctgcta ttttaatata gagaaacaac
      841 agtatattgt aaagtaaatg tcaaatatag ttttaaaagt aaaattaatg ttaaaaagta
      901 aatataattt tacacttgct taacttaatc ctaatataga tttaacttaa aaaaagtaga
      961 ataaaagtac aggaaatttg atgaagacga attataaaaa attaaatttt gaatacagaa
     1021 cgaaaatcga taagtatatt ttaatgggag ggtattatga tagtaaaatc tagattagta
     1081 ggtttagaaa aaaatgacaa taatggcaat aagacaaaat acgtaattga aaaagtagca
     1141 aaaaatattt ttctacttag tgctttagta gcagttgcaa gtttactttt gataatagga
     1201 tttgtatttt ataaaggatt aagaccattt atatttgaag gatattcatt tatagacttt
     1261 ttaactggtg cagattgggt tccttctgcc aataaatttg gaatatcatc tatgatagta
     1321 gcatcaatat ttgcaacact tggagcactt gttataggag tacctgttgg tatattaacc
     1381 tctgtattta tagcagaagt agcacctaaa agattagcaa aaataatgtc accagcagtt
     1441 gaacttttag caggtatacc ttcagtatta tatggtgtat ttggtcttgc agtgatagtt
     1501 ccaactatac aaagtatatt taatcttcca aaaggtcaaa gtttgttagc aatagtaata
     1561 gtattagcag taatgatgtt accaacaata atatctgtat cagaaacagc aataagagca
     1621 gtaccaaaag catacaaaga aggttcatta gcacttggtg catcaaaaat agaaacaata
     1681 ttcaaagttg tattacctgc tgcaaaatct ggagtattag ccgcagtagt gttaggagta
     1741 ggaagagctt taggtgaaac aatggcagta atacttgttg caggcaactc accagttatg
     1801 ccaactgcaa taacagatag tgtaagacca cttacaacta atatagcact tgaaatggga
     1861 tacgcatttg gtacacatca agaaatgcta tttgcaactg gagtagtatt gtttacattt
     1921 atattaattt taaacttagt tttaaataaa ctttcaaata aggcggtaaa ttaagatgag
     1981 aaagttcaaa gagaatctat tgaagtcatt agtatatcta tcagcactat ttacaatagt
     2041 atcattagta gttatagtgg gatttatatt tataaaggga attggcaaca tgaattttaa
     2101 tttcttattt agcaattatt cagcatcagg tgatggtggt atacttccaa tgataattac
     2161 aacattatat acagtagtag tttctatagt tgtagcaact cctataggaa tattggctgc
     2221 tatatattta caagaatatg ctaaaaaagg aaaagcagtt acagctataa gatttgcaac
     2281 tgaaagttta tcagggatac catctatagt atatggatta tttggaggaa ttttctttgt
     2341 agttacatta aaaatgggat attccatagt tgcaggttca cttacagttg caataataat
     2401 attaccagta ataattcgta caacagaaga agctttaaaa acagttcctc aagcttatag
     2461 agaatcatca ttggcattag gagctacaaa atttcaaact ttatataagg tagtacttcc
     2521 aagtgcaata ccaggaattt tatctggagt aatcttatca gttggtcgta taattggaga
     2581 atcagctgca atattactta cagcaggtac tgtagccaag atgccaggtg gaatatttga
     2641 tagtgcaaga acattaactg tacactcata cttactgact aaagagagtg gagatatagc
     2701 aacagcagcc agtataggaa ttgttttaat agtaattgta ttagcactaa acatgctagc
     2761 aagatttgtt gctaagaaat taaataaagc taattattaa atttaagagg tgtgaataat
     2821 ggaattaatc gataagataa aaatgagtgt aaaagatttg gatttatttt atggtgataa
     2881 gcaagcatta aagaaaataa atatggatat aaaagagaat aaggttactg ctttaatagg
     2941 tccatctgga tgcggaaaat caacttttat aagaacgtta aatagaatga atgacttaat
     3001 cgaagatgta acaattaaag gaaacatttc tgttgatgga gaggacatat atacctcaga
     3061 tgatgttata aatcttcgta ctaaagtagg aatggtattt caaaagccaa atccatttcc
     3121 tatgagtata tatgataatg tggcatatgg tcctcgtaca catggactaa gagataagaa
     3181 gcaattagat aaaattgtag aagaaagctt aaaaggtgca gctatttggg atgaggttaa
     3241 agacaggctt aaatcatctg cactaggact ttctggtggt caacaacaac gtatatgtat
     3301 agcaagagca attgctatga gaccagaagt tattcttatg gatgaaccta catctgcttt
     3361 agacccaatt tcaacattaa aagtagagga acttatagag gatttaaaaa aagactacac
     3421 aatagtcata gttacacata atatgcaaca agctgctcgt atatcagatg aaacagcttt
     3481 tttcttaaat ggagaggtta tagaatttag cgatacaaaa actatgttta ccactcctgt
     3541 agataaaaga acagaagact atataacagg tagatttggt taaaatatat aactataaag
     3601 gagttagaca atggtcaata atagcttaga tataagtatt gatactttaa aagaacaaac
     3661 agtgcttatg atggaaaaat gtgaagaagc aatagaaaag tctgtgactt gtatgataga
     3721 gaaggattta gagggtgcaa gaaaagtagt agaagatgat gaagaaataa ataaattaag
     3781 agaagaaata agagataaga gtatagagct aatagtatta aagcaaccaa tggcaaggga
     3841 cttaagattt atttattcat taggtaatat atctatagag ctggaaagaa taggagatta
     3901 tgcagcaaat atagctagag aaatgttgaa gataggtgga gaggcttata taaaagagct
     3961 aatagatatt cctaagatgg caaaagaatg taagtctatg atgtctcaga ctagagaagc
     4021 tttaatctta tttgatgcaa gtatagctta tgatgttgga actaaagatg atattgtaga
     4081 tggattgtat gaacaagtac aaattgattg tttaaaaata atgcatgaaa actcaaatac
     4141 catagaccaa ggggtaaaac ttctacttat aggaagatac cttgaaagaa ttggagacca
     4201 tataacaaat gtatgtgaga agataatttt tgcaattgag ggtaaaatgg ttgagatagg
     4261 ataaaaaact gctttaaagc agttttttta ttgtctaaat taagaaagaa ttgataaaaa
     4321 aatatgatta atgggtaaat ttaaaaaatt gtgatatact aggaacatat taaatatgag
     4381 gatgcgtatt ttatataagg tgctttttag gaggtggaac tttgaaactt caaaagatgg
     4441 gtatgaggac tataaaaaca ggaattagtg tcactttatg tatgattctt ggtgactatt
     4501 tagtagagaa tatgttttat tctctggtag catgtataat ttctgttcaa gatacagtaa
     4561 aaggttcttt aaagacggga atcaatagag tggaaggtac tattttaggt ggtataatag
     4621 gattcatatt tgccatcatt aaagctggag acccattttt atgtggtcta ggagtaatgt
     4681 gctcaatata tttatgcaat ctgtttaaaa taaagtcagt agtagttgcc tgtgtaacct
     4741 ttctggctat acatctaggt gtaggaaata gtgaccctgt gtattattct ttacataggg
     4801 tgttagatac atctgtgggt gttttgtttg gagtacttgt taattattat gttgctagac
     4861 caaattatct aaatagtgtt atagatgagt ttaaaaagat agaagaactt tcaatgttgc
     4921 ttattgaaga taaaattctt agaaaagaaa atttaaatgt agagaaattt gaagaagaag
     4981 tagaaaaatt agaagaaata tattcaaaac ttattgatga gttggattat agtttgagtg
     5041 aaactaatat agaaaaaatt gagaatgcct taaggatatg tagagaaata cattttcata
     5101 tgcagtctat agagttactt gaaaataaac tttatttgaa agagcataca tataaatacc
     5161 ttaagaaaac atataatgtg gataagctag actggaatat agatgaagaa aaaagccctg
     5221 tattcaacta tcatctaatg aaaataatga ttgaaataga gttgttgaga aaactaaata
     5281 aatcatatta ataatagtag ttaaataata aataaaaaat agtatctttg taaatattaa
     5341 acatatataa gtttaattta tggaggtact attttttatg aatactagaa atgacaattc
     5401 taaaatacta gaaatatata aaatgaagct ttttggcgaa cttgtaaaag cagtgtggaa
     5461 tgatgacata gaggatattg aaaacttacc taataaaatt ttaaatgaaa atttaaagga
     5521 taatttaaat aattatttta gtggaaaatt tgataaagat actatggtaa atttaattag
     5581 ggtaactatg ggattggatg tgacagaaga tacagataaa aatttaaaag agtcactaca
     5641 tgaagctgta aatttagatg aagtcaaaag accaataata tctgtaatat cagatgcttg
     5701 caaatattgt gatgaaaata ataaggaaga tgagtcatgt aagataagaa gtaagcatat
     5761 taattgtaat gaggaagatg tatgtagtgc ttgtggtgat tgtgtatcaa gatgtaagtt
     5821 gggtgcaatt tcagataaaa tagaatttat accaatgata aaattgttaa aagatataaa
     5881 gagccctgta tatgcaatag ttgcaccagc ttttgtagga cagtttggga aagatgtaag
     5941 ccctggtaaa ttaagaactg cattaaaaaa gataggattt gaagatatga tagaagttgc
     6001 tttggcagca gatatgctta ctgcaaaaga agcatatgat tattatgagc atatgaaaaa
     6061 ggacgaagaa ggatgcttta taactagttg ttgctgtcct atttgggtta gtttgataca
     6121 aaatagtttt cctcaaatat tggagaatgt atcaccatcc gtttctccta tgattgcttg
     6181 tgggagagct gtaaaaattt taaatccaga tgctaaagtt gtattcatag gtccttgtat
     6241 ggcaaaaaag aaagaggcaa tgttagaaga tataaaagat gcagttgatt ttgtgcttac
     6301 atttaaagag ttgcaagaaa tatttgatgc attagagtta aatccagcaa taatggatga
     6361 gagtaataga gtagaggcgt ccctttcagg tagagtttat gctagaacag gaggtgtaag
     6421 taaagcagtt aagttatcag ttagaagaat tgacaaaaat ataaaatttg aagaaaaggc
     6481 atttgaagga acaaaagagt gcatggacgg tctaaagaaa gttctaaata aagaaataga
     6541 agctacattt atagaaggaa tggggtgtgt aggaggctgt gtaggaggtc cgaagcgtat
     6601 tttaagtgtg gaagaaggta ctaagaatgt tgatgaatat agtgaaagta cgaatatgga
     6661 aactccattt gataatttaa atgtattaca atttttaact tctttgggaa tcaaaagagt
     6721 agaatcttta ggagaaaaag aagaagaaaa ggttaaaaaa atatttagta gagatataaa
     6781 aagtaataag aaagagaata aatataaaaa ataacttttt aagtcttaaa cttatgcttt
     6841 tataaaaggg agatatgggg gatgaaaatt aaagataata aggctgtttt tcatgtattt
     6901 ataaggatat ttcttatagc tgttttaatt ttaactggaa cattattgga ggaaaaaatt
     6961 aataatgttc cagttgaaaa tataagttca aaaggtgaaa atacactgga actgctttct
     7021 caaagtaata ctttaggaga aatcatatac aaagcatctc aagaagaaac aaaaggtcca
     7081 ggggctactt ctgaaaaagt agcatacata acaatagatg atggtccatc aaaatataca
     7141 aatgcactac ttgatatttt aaaagaaaat gatgtcaaag cgacattttt catgcttaat
     7201 cagaatatga aaaaccacaa ggaagaagta aggagagtct tggaagaaga aaactcaata
     7261 ggttttcata gtgtaagtca tgatatacat aagttatata aaagcccaga agtaacagtt
     7321 ggggaattta atacatgcaa taatactcta caagaaataa ctggtcagag ttcaaaatta
     7381 ataagattac catacggaag taaaccatat atgccagaag gttcatataa taaacttatg
     7441 gcaaatgatt atttaatttg ggactggaat ttggatacac aggattggaa gtcttctaca
     7501 agtcagatag taagtaatat attatattat ggaagaaaaa gagatgaatt agtagtatta
     7561 attcatgaga aagagcaaag tttaaatgct ttaaataata ttataagaat cttaaaagag
     7621 agaggatata caatattgcc aattacagaa aatataacac ctaaaaattt ctggcaagaa
     7681 aacttataat aaatttaaat atagtaaaag ataaagtaaa ttatttataa aataaaacat
     7741 ataatataaa aaatacgaat aagaggtata ctgtaaggat aatataaaat taatgagaaa
     7801 gcattaaaat taaaataaaa taatcttgca attattgaca ttataagaca gaataactgt
     7861 aaaaacatat tttaaaatat aacaactata taaaagagaa tagacataat taagaaaatt
     7921 cagaaaaaat aaaataaaaa taattgacaa agagaataat atttgtatat aataatatgt
     7981 aatattaaag actgagaaga agaggagtaa gtaaatacta ctaaaagaga ggaaaattca
     8041 ctggctgtaa gattttctaa gtataagatt tactgaatgt agctttggag tttctaaact
     8101 gaactataag taagtttaga cggttagaac cgttaaattc tttgagagtc aaagttgata
     8161 aaataacatt aaccaataat ttaatgatat gttaatgtat tttaagcttt ggaattaagg
     8221 tggtaacgcg agcttttcgt cctttttaaa gaggatgaag agctcttttt tatttctaaa
     8281 gatatttaaa aaatttattt ataaaataca ggaggcagga caatggaaaa tacgaattta
     8341 tcaaaaactt ataacccaaa agattttgag gctaggctat ataaaaaatg gatggatgaa
     8401 gggtatttta aatctaaacc aaatccagat aaaaaaccat ttacaataat gatgccacca
     8461 ccaaatataa cagggcaatt gcatatggga cacgctcttg accatacact tcaagatata
     8521 ttaatcagat ggaaacgtat ggatggatat gaagcttttt ggcttccagg aactgaccat
     8581 gcttctatag caacagaagt taaagttgtt gaaagaataa aaaaacaaga aggcaagaca
     8641 aagtatgaaa taggtagaga agaattttta aagagagcct gggaatggaa agatgaattt
     8701 ggaggaaaaa tatctaatca gcttaaacaa ctaggtgatt cttgtgattg ggataaagaa
     8761 agatttacta tggatgaagg gtgtaatgaa gcagttatag aattttttgt aagcctatat
     8821 gaaaaaggtc acatatacag aggaaataga ataataaact ggtgcccaga ttgcaaaact
     8881 actttatcag atgcagaagt tgaacatgaa gaacacgacg gaaactttta tcatataaag
     8941 tacccactaa aagatagtga agacttttta gaaatagcta caactagacc tgagacaatg
     9001 ataggagata ctggtatagc agtaaatcca gaagatgata gatataaaca tttaatagga
     9061 aaaacagcaa tacttccatt agtaggaaga gaacttccaa tagttgcaga tagttatgtt
     9121 gatttagagt ttggaactgg tgcagttaag atgactccag cacatgaccc taatgatttt
     9181 gaagtaggat taagacataa tcttgagcag ttaaatacta tgaatgaaga tggaactatg
     9241 aatgaagttt gtggaaaata tgaaggtatg gatagatttg agtgtagaaa agctatcgta
     9301 gcagacttaa aagaacaagg ttacctaatt aagataaaag agcataatca taatgtagga
     9361 acttgttata gatgccatac agtagttgag cctagattat cagagcaatg gtttgttaag
     9421 atggaagaac ttgcaaaacc agctatagat attcttaaaa aaggagaatt agaatttgtt
     9481 ccagataaat ttgataaaac ttatttacaa tggctagaaa atataagaga ttggtgtata
     9541 tcaagacaat tatggtgggg tcatcaaata ccagcttatt attgtcaaga gtgtggagaa
     9601 atagtagttg ctagaaaaat gccagataag tgtcctaaat gtggaagcac tcattttaaa
     9661 caagatgaag atgccttaga tacttggttc agttctgcat tatggccttt ctctactcta
     9721 ggatggccaa ataagactga agctttagat tattattatc caacaagtgt acttgttaca
     9781 ggatacgata taatattctt ctgggtagtt agaatggcat ttgcaggtat gttctgtatg
     9841 aatgaaaagc catttgacca tgttttggtt catggattag taagagattc tcaaggtaga
     9901 aaaatgagta aatctttagg aaatggtatc gacccattgg agataatcga gcaatatgga
     9961 gctgatgccc taagatttac attaacaact ggaaattccc caggaaatga tatgagattc
    10021 tatatggaaa gagtagaatt tgctagaaac tttgcaaata agttgtggaa tgcatcaaga
    10081 tttgtattta tgaatattga tgaagatata attaaaaaca tgactagaga aagtgttaaa
    10141 gaggacttaa ctctagctga taaatggata atatcaagag caaataatat tgtaaaagaa
    10201 gcaactaata acatggataa gtttgattta ggaatagcac ttcaaaagat atacgatttt
    10261 acttggtcag aatactgtga ctggtatatt gaaatggtta aacctagatt atatggagaa
    10321 gatgcaaatg ctaaatctgc agcattatat acattaactt atgttcttga aaaaatatta
    10381 aaattattac atccatacat gccatttata acagaggaaa tatatactca tcttcctact
    10441 gttgaaggat gtataatagt atctgaatgg ccaaaatata atgaagaaga taacatggct
    10501 gaagaagaag atatgatgaa cttattgatg gaaggtataa gaagtataag aaatgtgaga
    10561 gcagaaatga atgtaccacc ttctaagaaa gctaaattga taataatccc aagtgaagaa
    10621 aaaatagaag ctattgagct tggtaaagac tattttataa ctttagcatc tgcttcaaat
    10681 gtagagatag caaaagataa atctaatgtt ccagaagatg cagttggagt agttattgat
    10741 ggtgttgaaa tatttatacc tcttaatgaa ttagttgatt ttgaaaaaga aattgagaga
    10801 ttatctaaag aaaagaaaaa gttagaaggc gaaataaaga gagttaatgg aaagcttgca
    10861 aatcaaggtt tcttagcaaa agctcctgaa agtttaattg aagaagaaaa agcaaaaaaa
    10921 gaaaaatttg aagagatgat taaatctgtt gaggaaagat taacaaattt agaatctaaa
    10981 ataaaataaa tataagaatt agtaaaaaca tacaaaaagt atatattcta tactttttgt
    11041 atgtttttct ttatatatat acaaatatga tgttataatt ctttacagat gtgttatatt
    11101 tttattttat gttgtgaata tatgaatttt atgtcttaaa atttataagc aataaaaat
//
LOCUS       DAYASV010000121        11141 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYASV010000121 DAYASV010000000
VERSION     DAYASV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.123"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            123..1298
                     /locus_tag="ACGI68_002991"
     CDS             123..1298
                     /locus_tag="ACGI68_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0139417.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1471..2868)
                     /gene="asnS"
                     /locus_tag="ACGI68_002992"
     CDS             complement(1471..2868)
                     /gene="asnS"
                     /locus_tag="ACGI68_002992"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFH0139418.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(2945..3178)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3347..3541)
                     /locus_tag="ACGI68_002993"
     CDS             complement(3347..3541)
                     /locus_tag="ACGI68_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HFH0139419.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3814..5487)
                     /gene="cspC"
                     /locus_tag="ACGI68_002994"
     CDS             complement(3814..5487)
                     /gene="cspC"
                     /locus_tag="ACGI68_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid germinant receptor pseudoprotease
                     CspC"
                     /protein_id="HFH0139420.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLEPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5497..8895)
                     /gene="cspBA"
                     /locus_tag="ACGI68_002995"
     CDS             complement(5497..8895)
                     /gene="cspBA"
                     /locus_tag="ACGI68_002995"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional germination protease/germinant
                     receptor pseudoprotease CspBA"
                     /protein_id="HFH0139421.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9062..10258)
                     /locus_tag="ACGI68_002996"
     CDS             complement(9062..10258)
                     /locus_tag="ACGI68_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0139422.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSISYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10279..11100)
                     /locus_tag="ACGI68_002997"
     CDS             complement(10279..11100)
                     /locus_tag="ACGI68_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0139423.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3538 a         2051 c         1300 g         4252 t
ORIGIN      
        1 acaattttag attgttacac acaatatatt ttgatataat taaatcataa acgaagtatt
       61 ccaaatatct aatgatagaa aggaatactt atttttatgc tttattttta atggaggctt
      121 atatgaactt agaaaaaata aaaaaatttt ttggatttcc tctagacaaa aaacacaaag
      181 acaatgttga gaaagaactc tgcaattatc tttatcatac gggtaaaatc atttgcataa
      241 taatagtaat atctcaaata attatgatga tatcaatctc agctagagaa ggaggccctt
      301 ttgtcacacc aagacgccaa tggtattttt atatgtatgt aacactattt tgtgtatctt
      361 ctgtctttat gtttgtattt tcttaccttt ggaataaaaa aaaggattgc tgtacgtttt
      421 atataaattt atggataatt tatgtgactt ttttctgcct atggggttgt gtaattactc
      481 tacttgacca gttaggtgga aatgacctaa gtgtatttag ttatattatg ttagcagctg
      541 cagctttctc agtattaaaa tttggagaat cagtaatagt ttttggagga tgctttttgt
      601 ttcttaattt attcttacct gggattcaaa ctataccagg caatgagttt agtaatattg
      661 tcaacacatt ttttataaca tcccttgcca tttttgtttc tacaatgcta tatagaaatc
      721 gtgtatttac ttactatgat aaacttttaa ttagtcaaca atatgaagaa atatctcata
      781 taaataattt acttaacgac aaagttatga cagatgacct tactaaaatg aacaaccgta
      841 ggtaccttga agatattcta caaaaaaaat taatcagcca tgcaaatcaa gaacctataa
      901 ctggaatgat gatagatata gattatttta aacaatttaa tgataattat ggacatcaaa
      961 agggagacta ttgtctacaa aatattgcca aagtaatttt agattttaca agttctgaaa
     1021 atgaacatgc tatacgatat ggtggtgagg aattttttat atgtattttt aactgtaatg
     1081 aggcagatgc taaacttaaa tcagaatgtt tacgaaaaat tatagagtcc tgcaattttg
     1141 agcgtgatga tgttccttta aaatgtgtaa ctgttagtat tggagtttat acaaagaaaa
     1201 ttggaaagac aataacatta aatgagttta taagtaattg tgataaggcc ctatatactg
     1261 caaaagaaca aggacgtaat tgtgttgcat tttattaaaa ggttacatac tattacataa
     1321 acctataagt attatatcgc aaaataaaag taatcaaatt atatatttat agtacaataa
     1381 aaataacttg catatctcta aaatagataa atatgcaagt cattttagtg tttatactta
     1441 aagttatata agttttatta tatataatta ttaaaattca gcatttttag gagttcttgg
     1501 gaaaggtata acgtctctta tgttagacat acctgtcata tacataatta ttctttcaaa
     1561 tccaagacca aatccagagt gagtagcagt accaaacttt ctaagttcta aataccacca
     1621 ataatcttct tctcttaatc ccatttcatt aattctatct aacaatacat cttctctttc
     1681 ttctctttga gaacctccta cgatttctcc aactccagga actagtaaat ccattgctct
     1741 aacagtttta ccatcttcat tcattctcat atagaaagct tttatatctt ttggataatc
     1801 tgtaacaaac actggacaat tatatatttg ctccgttata tatctttcat gctctgtttg
     1861 aaggtcacat ccccactcaa caggatactc aaattcatgc ccagattcaa ttaataattc
     1921 cactgcttta gtatacgtta ttctaataaa ctcagagttt actatcttat ttaatctttc
     1981 taacaatcct ttatctataa aactattaaa aaattccatt tcttcaggag cattttctaa
     2041 tacatagctt ataacatatt ttatcatatc ttctgcaagt tccatgttat cttcaaggtc
     2101 tgcaaaaact atttctggct ctatcatcca aaattctgaa gcatgtcttc ctgtataaga
     2161 gttttcagct ctaaatgtag gtccaaaagt ataaacgttt ctaaaagaaa gtgccattat
     2221 ctcggcattt aattgaccac taactgttaa attagcctct tttccaaaga aatcttgagt
     2281 atagtctatt ttgccatctt cagtttttgg aatatcattt aaatccatag ttgttattct
     2341 aaacatctct cctgcacctt cacagtcact acctgttatg attggagaat gtgcatatac
     2401 aaagtttctt tcttggaaga atttgtgtat tgcataggca gctattgatc ttactctaaa
     2461 tacagctgaa aaagtattac ttcttggtct taaatgtgct atcgttctta aatattcaag
     2521 cgtatgtctc ttcttttgta gtggatacga actatctgat tccccttcta caactatgct
     2581 ttttgcatgt atttcgactg gttgtttagc cccttctgta gatactaact ctccctctac
     2641 aagtatagat gagcttattg ctaattttcc tacctcttta aaattttcta ctttctcatc
     2701 aaatactatc tgcatatttt taaagaagct tccatcattt agctctataa atccaaaatt
     2761 tttagatact ctagatgttc ttatccatcc agctactttt acagtcttcc ctatgtactg
     2821 ttctttttct ctgtacaatt cttttactgt tataaattcc ttttgcattt taaatcctcc
     2881 tagtataatt gttattaatt tcttttaaat ataatttcaa taaaaaaagc ccttcatccc
     2941 tatataaagg gacgaaagac tattactctc gcggtaccac cctagttgtc ataatgacct
     3001 cttagttaag caatgcttaa agcctataac ggtgccttcc gtctaagtct acttactaaa
     3061 taagctttcg attagaaact ccaagatgtt cttcaatata aacctcgtat tgagctttca
     3121 ccatcctcaa ctcgctagaa ctacatttta tatctactct tctcttcaca gtatttatct
     3181 tttattattt ctgctaatta acaaaattat accaatttat aaatttttag tcaataaatt
     3241 tatataattg atactgattg ttatttaaaa tataataatt ctaccttaca taatgataat
     3301 aaatacttat cttcattact tctttattac ttcaatttcc aaaattttat aatttacttc
     3361 cattgtcaac ccagtctttt gcttcttcga cacccttaat tgaaggtatt gaaactttac
     3421 tatgaggttt taatttttca acatcagccc atgctgctgt cgactcattt ccaactaaaa
     3481 gtgaactttt aagtctttta ttatctttac cttttaatgc tcttgccttt ttactttcca
     3541 aaactatcac ctctattatt tattttgtaa tagttttagc aattttacaa ataaaattca
     3601 taagtctaaa ttataattat ttttaaatta acataatatc ttttcaatac ttaatttata
     3661 cctatttata tacaaaatat ttactttgtg tattcaaaat ataaaactgt tttttaattt
     3721 tcatatttat tttttatgct atttttctac tctatctaaa gtatttgata caattaaact
     3781 aataatcaat tataatttta cataggttct tatctataga gtatttgcta tctgttgaat
     3841 cgtatttttt aaatttaaaa tcccatatcc ctgcgataca tttggatatg tatatatctc
     3901 aagttttgta gcacccaata ttaaatatgt ttttaaaact tgtgtaaaca atgataatct
     3961 cggtacatta tcctgttttt ctaagtattc catcaataag gctaaaacac ctgtgactat
     4021 agaactgctc actcctgttc ctgttcctgt attatatgtt ccatttttat atgtggatat
     4081 tatatctaca cctgaagcta ctatatctgg cttaattcct cttcctctaa ttggtccttt
     4141 cgaagaacca atccacatac tatcagtctt attgttaaat gtaccaactg taattacatc
     4201 atcactagca gcatacatag ttatagtagc tactgaatca ggctctaaaa accttgtgtc
     4261 tggagctatc aaatttttat ttggcaagta tatgtcatat tctccattta ttataagttc
     4321 tgaagtcaat cttaaaatcc atactccagg ttttatatct cttaatctta tctccaaatt
     4381 ttcttttcca gaggtaatat atggataaat aaatctcatt gcataagtag tattttctaa
     4441 gttaaatttt cctctatata tataaaagtc tggagaatac cttatatcat gactaacttc
     4501 acctgaagga gatattatct gtgctcccac tttatctgga ccattcgtat ttagtgttat
     4561 atcaagagca tagtcatctc catcttgtat tattacatct tgaacttctc ccacactgct
     4621 aaatctgcca gaataatgaa tatctgtatt cccttgattt cctgcaccac tcactactac
     4681 cacaccagca ctactcaata tattaaaagt atctagtatt gaagtagttg ctacagcact
     4741 tgacttaact ccaatagtca aatttattat cagtggttta ttttctgttc ttgctatgtt
     4801 agtaacatac gttatagctg ctaaaaaatc tgaaacactg taatttatcc ttccagcata
     4861 ataagtatct gtatatgatt ttaatttaac tactattaag tctgattctg tagtaatacc
     4921 tctgtactga gaatttatcc ttccatttcc aactaatata ccagaaacta aagttccagt
     4981 acctatatta tcttgactca aactcccatc atttctattt atggctatat ttaactcact
     5041 tcttgtaaat tcacttccaa aaataaaacc ttctggaggt ggatttgtat ttgcttcttg
     5101 gtcccataaa tataaaactt tactagttcc atcatcattt ataaaatcag gatgcaaata
     5161 atctatccca gaatctataa ctgctaataa aattcctctc cctgttatat cattatatgg
     5221 attttcataa atataatcag tttcagctgc tgttgtaatg gtttctccat tatttacatt
     5281 attagttatt tcaattagac tgctcattgg ttcagattct tcccaccaag caacttgtat
     5341 tatattattt aaaatagttt catcaaaatc taaaggtaca tatattactg caagctgact
     5401 atttaaaacc atatacctat ttattccatt ttcttgtagg gcagattcta tatcaccttg
     5461 ataaattata caataagatt tttccataaa tccctcctat cttaaaacat caaacattcc
     5521 tctaacatcc aagagcccat atccagaatt tgtatttgga tatactgtgt ttgaatcttt
     5581 tctagcacca gcttgcataa aagtttttat tttttgcaca tatgcttggt ttggatatct
     5641 accgtcaaca aaagtatatt gaaaatacat tgcagcagca ccagctgcat gtgcacttgc
     5701 tgctgctgta ccagttatag ttgcatacgt gttcccagga taagcagcta ttatattgac
     5761 acctggtgct actatatctg gttttaacct gtcttcaata gttggccctc ttgatgaact
     5821 ttgccataaa ctaccattaa tggtattgta ggctcctatg gttattaaat catcttgaat
     5881 cgcaggataa tttatagtgt aaaaagggtc tacctctcta aatctagttc cactttttaa
     5941 aagttctcta tttggaaggt ataagttata tcttcctgtt ataatataaa ctcctactaa
     6001 tcgaatcttc catacacctc tttttgcatt ctttaaagta acattagtaa attgctgtcc
     6061 agaaaaagtt gttgggtata tatatgttat agaatattca gtcccctcta aatcaaataa
     6121 acctgttact ttattataat ttgaaattcc aacactttta ctttcttcac cagtgggaga
     6181 tactattatt acatcagctt tatctggtct atttaaccaa agttctaatg ataattcttc
     6241 ctcgtcttca tttaactcta attcaacttc aacagaacct ccaacatatg gtattattcc
     6301 agatgtatgt gtctgtgtat tgccctcatt tcctgctcct gccgttatac ataaccctct
     6361 agtaaagaac gctttttcac tattactcct attggttaaa cctgctaggc tactggtacc
     6421 taaagacata tttataacta atggtcttcc aagttcaaaa gcttttttat atgcatactg
     6481 agatgcagca aacaacattg cactattata aaaatcatct atcttgccga gttttattat
     6541 tattagctca gaatcttctg ctatacctgc atattcacta tttacatttc caagccctgc
     6601 acatatccca ctcaacatag ttccttgacc aacttcatcc tgagatagac tagggtcatt
     6661 ttctgcgata gctcgattta tatcttctcg tgtatattct gtcccaatat aaaaaccatc
     6721 aggaggagtt ccttcttttg tttggtccca taaatatact atttttgaag tgccatctgg
     6781 ataaataaaa tctgggtgta gatagtctat tcctgtatct gcaattgata ttagagtccc
     6841 tcttcctgtt atattaatat taggattatt tttaaagaaa tttacgccca tttcttctgt
     6901 tgcaactaca cctccaaaag tacctcgatt aatttctcca agtaatgtca tctttgtagt
     6961 cgatacagtt cttatgattg aaggaagttg caaaactcgt tgtatgctat tatagtctgt
     7021 attatttata gatagtacac ctaatgaatc acttatctta aaaaaccttt gactcatacc
     7081 taacccagct agttcttctt caaattcagg agtatggact atatctattc ctgatagtat
     7141 tcctgattgt ctacttgcat ttttacttat ataaatttca ttctctaagt cattatgtat
     7201 ttgcatatta taaccatcta taaacttata actattttcc tcatctttaa atgacttatt
     7261 aatacttcta tatccaatat cctctgtttc taaatcttga tttatattag atagagtgta
     7321 caaatctata tctgataaat ttaaaaaacc aaatcctgat gaattatttg gataagattg
     7381 attacttaat ctccttgctc cctttagaag taatgccctt aatttctgtg aatacaaaaa
     7441 taaatcattt ccatttacaa ttccccattc cataaacaat gaacaaaccc ctgtaacatg
     7501 aggtgtagcc atgctagttc cagttaatgc tccacttgta cctcctggta aaaaagatac
     7561 tatatcttca cctggagcta ataaatcagg tttaaaaaca cctaattgtg tatctccttc
     7621 accagaaaat atagatacta tatctgttct tgaattaaaa ctacctactg ttataactct
     7681 acttgctgtt ccaggaactg ttacagtaag ctcttgagta ggaatcagaa atctagtatt
     7741 tctatttaga ccttcagaag ttggcaaata tatatttaca ttaccagtta ctatatctat
     7801 tggttcaaat acaattttcc ataacccagg agttatctgt gtatttgaac ttaattgtag
     7861 agtaactctt cttgtgagag aatacggtgc tatagggtaa aaataaccag ttattctagt
     7921 ttcacccaaa gtattcctta tctcacctga agttagagag attgcttgtg tttgattatt
     7981 tgatgggttt accaaatgta cactaaaatc atcaacaaaa tctggccata tatttatgtt
     8041 taatatacgc tctccttccc caactataaa ctcaacttct tctgtaatat tattttgaag
     8101 cctaatcctt ttatgacctc ctttatctgc attatttcca gctgctacaa ctatattgtt
     8161 tttccaaaat aaacacatat catctatata ttgttcaaat aaagatgttc ctctatgtga
     8221 accttcatta cttccataac ttatatttaa tgtaacaggc attcttaatt ctaatgccct
     8281 atctaaaata aatttaattg ccctcataaa ctctgtactt ctcgaaaaaa tatcggtctg
     8341 tatatttcca actctaacca ctattattct ggcatcactt gcaatagttg cacaaatacc
     8401 tgcaacatgt gttccatgta agctagttgt tgaaataggt atatacatac ttccatctat
     8461 tgcattattt atatcttcat tagtatataa tgttccttct ctaaaacctt ctggtggatt
     8521 tccttgaata gattggtccc aataatataa tatcttagac cttccatcac tatctctaaa
     8581 cacaggtaga gtataatcta ttcccgaatc aattatacca attatagttc cttttccagt
     8641 taaaccagtt ctatttttaa atcctgttat tcctgtactt gaaaaacttt gcacatcctg
     8701 agtctgtaat ataaaaggct tttctataaa ttcaatttct ggataagtta aaagtatatt
     8761 tacatcttct tcattacttg atgttattat tgcatatgat gaattaagta tctctactga
     8821 tacacctaat tcttcttcta acctcaatat atctccattg tattttacaa ttaattcata
     8881 atttattata atcacctcga cttctaaaat tattattaat gtatatgttt tgtgtgagtc
     8941 taaaatgtat tagactatat aattttttaa gtttcagtta tacaatttat aatagatata
     9001 ttaaaaaaat aaataatgat ggtacaattt tgaataaatc ataccatcat ttatatttac
     9061 tttatcttga aaccattaaa tttttcaatg ctttctcaag accttttaca gtcaatggaa
     9121 acatattaaa ttcactttgt atcattcttt gagtttgata ccaataacta attgaatccc
     9181 atccatctct aagttctgga ttcatccata caattttact gtactttctc ttaaaccttt
     9241 taagccactc tataccaggt gtataattat atggaccacg atagttacca ccaatatgaa
     9301 gcaactcaga aggcgacata gaagcatctc caacaattat aactttatag tttttatcta
     9361 tattttttaa tatccattca gtattaatag atttacttaa ccaacattca ggtgttgtaa
     9421 ataatctatc ataaatacag ttatggaaat aatatatctt tacatcttta aaatgatttg
     9481 atttacttac agactgaaat aatgtattac ataaagtgct ataccctctc attgaaccac
     9541 ctgaatccat aagcaataaa agcttcactg tattttttct tggcttctca aaaaataact
     9601 ttaaatatcc tgcattatta caagtctctt caatagtctt atccaaatca agctctgtct
     9661 ttggagcatc tattctagta gaaaattgac gaagtctctt tagtgcaact tgaaattgac
     9721 gcatatttaa aacttcatca tctctaaaat ctctgtattt tctttctcct gcaacttcaa
     9781 gaacagctct accataagta gaatttccac ctatacgtat accagtttgc ccttttcctg
     9841 aatgtcctaa ctctgaagtg cctcctgtgc ctatccaata attaccacca ttatgaggag
     9901 aatcttgttc ttttattcgt tcctccaatt tagctcttaa tttatcaaga tttatatttg
     9961 gattttgttc taatctttca aactcttcta aatctatatc tggtttttta agccaattca
    10021 taatctcttc tggaatttct tcttggtgtt ctataccttt aaaatactcc aaaaacactg
    10081 catcaaactt atcaaaatca ctttcacttt ttattaaaat catcctgcaa agatagtaaa
    10141 aatttgaaaa gtttgaatag cacagtcctt tatccaatgc ttcaattaaa gtcatccatt
    10201 catttaaaga tacatttaat cctcttgacc tcaataaata aaaaaattca ataaacataa
    10261 ttatccagct cctattaact ataagtgtct ttgcatagat tcaatatcct cattattctt
    10321 aagtagtatt cctgcaaaag gaacttcttt ttctattttt tcaataggca ttccactcaa
    10381 agttaatgct tgtatccagt ctattaactc tgatgtacta ggcttttttt gtatgtcctt
    10441 taaacttcta atccaataaa aagtagtcat tacttgttcc aataggtgtt cttctacttt
    10501 atcaaaatgt actttaacta tttcttccat catgtctcta tcaggaaatt ctatataatg
    10561 aaatatgcat cttcttaaaa aagcatctgg aagttctttt tccgcatttg aagttataat
    10621 aactattggt ctttgtttag ctcttacagt ttcttttgtc tcatttatgt aaaactccat
    10681 tttatccaat tcccaaagca agtcatttgg aaattctaaa tccgccttat ctatttcatc
    10741 tattaaaaga attacttgtt gatttgagct aaaggcctct cctagtttac catattttat
    10801 gtacttgcta atatcatcta caccttctcc tccaaattgg ctatcataaa gtctttgaac
    10861 agtatcatat acataaagcc cttcttgtgc ttttgtagtt gattttatat tccaaattac
    10921 taaatctttt tttaattcat ttgaaattgc ttgtgctagc atagttttac ctgttccagg
    10981 ttcgccctta atcaaaagtg gcttttcaag agcaatagca acattaactg atgccatcaa
    11041 ttctggtgat actacataat cactacttcc tctaaagtta gatatctctt tttttatcat
    11101 aaatcctact ccttttaaac agtattttct aaatgtatat a
//
LOCUS       DAYASV010000122        10996 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYASV010000122 DAYASV010000000
VERSION     DAYASV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.125"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..619)
                     /locus_tag="ACGI68_002998"
     CDS             complement(<1..619)
                     /locus_tag="ACGI68_002998"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFH0139424.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNI"
     gene            complement(712..1044)
                     /locus_tag="ACGI68_002999"
                     /pseudo
     CDS             complement(712..1044)
                     /locus_tag="ACGI68_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            complement(1122..1325)
                     /locus_tag="ACGI68_003000"
     CDS             complement(1122..1325)
                     /locus_tag="ACGI68_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0139425.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            1390..1527
                     /locus_tag="ACGI68_003001"
     CDS             1390..1527
                     /locus_tag="ACGI68_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139426.1"
                     /translation="MVFQPIPFLWTEPCHTGVSLYCGTGYGKTTVPKVIHKSLYSSSL
                     D"
     gene            complement(1715..1945)
                     /locus_tag="ACGI68_003002"
     CDS             complement(1715..1945)
                     /locus_tag="ACGI68_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139427.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1947..2300)
                     /locus_tag="ACGI68_003003"
     CDS             complement(1947..2300)
                     /locus_tag="ACGI68_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFH0139428.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            complement(2714..3163)
                     /locus_tag="ACGI68_003004"
                     /pseudo
     CDS             complement(2714..3163)
                     /locus_tag="ACGI68_003004"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3177..4103)
                     /locus_tag="ACGI68_003005"
     CDS             complement(3177..4103)
                     /locus_tag="ACGI68_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139429.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            complement(4228..5010)
                     /locus_tag="ACGI68_003006"
     CDS             complement(4228..5010)
                     /locus_tag="ACGI68_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFH0139430.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(5166..5786)
                     /locus_tag="ACGI68_003007"
     CDS             complement(5166..5786)
                     /locus_tag="ACGI68_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0139431.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5913..6581)
                     /locus_tag="ACGI68_003008"
                     /pseudo
     CDS             complement(5913..6581)
                     /locus_tag="ACGI68_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6652..7488
                     /locus_tag="ACGI68_003009"
                     /pseudo
     CDS             6652..7488
                     /locus_tag="ACGI68_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8160..8927
                     /gene="aroD"
                     /locus_tag="ACGI68_003010"
     CDS             8160..8927
                     /gene="aroD"
                     /locus_tag="ACGI68_003010"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFH0139432.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9255..9932)
                     /locus_tag="ACGI68_003011"
     CDS             complement(9255..9932)
                     /locus_tag="ACGI68_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139433.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10082..10399)
                     /locus_tag="ACGI68_003012"
     CDS             complement(10082..10399)
                     /locus_tag="ACGI68_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139434.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10421..10759)
                     /locus_tag="ACGI68_003013"
     CDS             complement(10421..10759)
                     /locus_tag="ACGI68_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139435.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3643 a         1741 c         1566 g         4046 t
ORIGIN      
        1 caatatttcc tgattgaata tgcttaatgt ttgattgaat ttttttatat ggccaatccc
       61 accatttaat ttctaataat tttgaaattg tatcattatt aaatctcttt tttataattt
      121 ttgcaggtac tcctccaaca atagaatacg gtggaacgtc attagtaact actgctcttg
      181 ttccaataat tgcaccatca ccaatattta cacctgacat aattatagaa tcataaccta
      241 tccacacatc atttccaatt ataatgttac ccttattgtc ccaagcgtcc gttatatggt
      301 tgatatcgag tttccattct tcataaaaga tgggaaaagt atatgtggat tttgatttca
      361 ttgagtgatt tccactagtc attagaaatt ttgcattaca tgctattgag caaaacttac
      421 caattattaa tttatcattg tttatagggt aatgatacaa tacattgttt ttctcaaaat
      481 ctcttgggtc attataaaag tcattatata ttgtgtaatc tccaacttct atattttccc
      541 ttgttattac atttttaaga tatacagttt gataatcatt acttcttgga tataccttat
      601 ttgaacttgg aatagtcata tttaaatctc tcctttaata tatattatgt ttttgactat
      661 tccactacta caatacatct cttcacagaa tcactccttg ttaagaatct tcataagata
      721 atggcgtcct tttttagtgt taagtcggat attctttctt agtccattct ttttagcgta
      781 taactgacaa actgtcattt tcttaccaat agtgtcaatg ctatcatcaa ggtcattttg
      841 tattgctttt tccttatccc ttaatgatat atcatcacgt tttccctttg ggggtttatc
      901 agtaggtaca agtttccaag agtaaacaaa ttgtggttta ccaaatgaat atacatactt
      961 atatgcgtat cttccatctt ttctctggct ctctccagtt cttaaaattc tatctttatt
     1021 atctcgtctt ttttcagttg acatgataag aagctcctct caataaccga attgagcttg
     1081 atacgtcata tgaatattat aacatataag gctcaatttc attagataac gtcaagtgta
     1141 tcaacgattt tctcaaattg ttttctcttt atttgaatgc gattgctatt cataatgacc
     1201 cagccagaat caaggttttc atcagcacgt ttacgtaatt ttttctctcc aatacgaaag
     1261 tattttgacg cttcttcaat cgtcaaggta tatttttccc aaataggtac atcagtattg
     1321 ttcatataat gtatcactcc tttctatgtt cgtagttcag aagtagatag atggctttga
     1381 taagctccat tggtatttca accaatccca ttcttatgga cagaaccgtg tcatacaggt
     1441 gtatcattat actgtggtac aggttacggc aaaacaaccg ttccaaaggt tattcacaaa
     1501 tcactatata gtagctctct tgattaaggt catggcgtat agtatttgtt ggctatctgt
     1561 atcacaaacg tatctatcta ctttattcag ttgtcaaaga acagtcggca ttgatttgcc
     1621 tataaaaaat cacaatagct tgtgggcttt tatagacaaa taattagtga ttctccatgt
     1681 accaaatagc ttgatacatg gagagagggc gagattaaga caaattaaat ctcaaaatag
     1741 cagtgattag ttttgttcct agcattcgct ttgtttcttc ttctacatga atatagggat
     1801 ttccgtattc atcaaataaa gttttctgtg aaagacgaat gatatagtgt tcaaaatgtt
     1861 taagtattac gtttattgca tttacatcgc catgtacagc aagtaaaata attgtaaaag
     1921 gtaatacttc tttagtaggt ttcatttcaa tgttcctgca tgagttcttt caactctttt
     1981 agtgcatttg ttttgtgata atgtatggta ttttctgcta aatccatcaa tgttgctatg
     2041 tcagcatttg tcatgttgaa aaagaatgaa agtagaatta tatcttgttt tctttttgta
     2101 agttttccga ttgcttgcgc cattaataca tcttcaattc gtatgttgta gtcgtaagaa
     2161 accatacaga tgtagttaga atcataatca tctaaaatac tcaatttact taattctgca
     2221 agtgacaaag attcgagaga taccaaataa tcatttctgc gtttattttc gtcataaata
     2281 tctctagcat agttacgcaa aactgtttta cagaaactgt cgaattgatg ttcaattgtc
     2341 ttttcatcaa ccataagatt tcaccctctt tccatcgcaa ggagttaaat aaccttgctt
     2401 actcctatca cggtgggagg gcgttaaatc ttcgataaat ttcaaaaaaa agagaaaaaa
     2461 atacacactc tgcctatagg gcaaaaatgt gtattcttta aaacgtattt aatttatatt
     2521 ggttgtaagc caccaccata ttgatgtatg gaaatatgca ttggctagct tttcataaaa
     2581 atacaaagat gcataagaaa ttaatagagg ggaaaaactt attctgttat taaaaagtag
     2641 gttaattcta ataaatgcta ttgcggtcga attaaactat atacatggca atcaatcatg
     2701 tttctattct ttattatgaa ttgtgacagt attcttcaat cttaaagcca catttttcca
     2761 atactttact tgactctcta ttgtaggaat atacattagc atgaattttg ttaaggttgt
     2821 atttttcaaa ggcaattttg catatttctt ttactgcatt tgttgctatc cctttgcctc
     2881 aatagttcct agcaagccag taacctaatt cagcattttt attaatagag gtatcttctt
     2941 caaatgttag gctgatactt ccaatagccc tttcttgata aaaaatagca tagctaagtt
     3001 gtttaacatc tgaagttgcc aaacaaaaac taacaaaatc tctagcgttt tgctccctat
     3061 aagggtgggg gaagttgtca cgaagattct ttgcaatttc tttatcattc gcaaactcaa
     3121 ctaaatcggt aatataagca taattccact ctataagttc catttttctt ctccttttat
     3181 gaaacagcta caagaaatct ttctaagcca ataccactta tcaaacagcg ttctctttta
     3241 tttccaagca tttgataagt ccagtcaaca aatccaccgt caactatttc aacggtttca
     3301 tttccttgtt tgaccgataa tttgaaattg atacctttat aataattatt atcaacatca
     3361 tcattatgaa ttgataactc tatattagga aaaataagtt tgatagtttc aaccattctg
     3421 tcgaaaaatc cgtcattatc tttgtatccg tttcttttgc gtagaataat agagaaacga
     3481 acattttcta tttcacttaa tatatcttta taatagttta agtgcttttc taacaacatt
     3541 ttttcacaag tgtaagaccc tgtatctata ccagatgaaa ccatacaata taagccaaaa
     3601 tgtgaatata aacctttacc tacaaaattt tgtgctctta ttaccctagc agtagtagcc
     3661 atgtggatag gtatggtatt attttcttct tttcttttta atttatcagc aataattatt
     3721 gccaacatat ttgaggggtc tgcaagtgtt tctgtccctc gtagagcact tactacgtta
     3781 tattgatcta cggagccaaa aacgctacag ctacctaatg gggcagaggg agaaagcata
     3841 atcgtcttga tatccatatt gaatgctttt ctcaataatt ttgattccaa agcatgaatt
     3901 tcttctggat ctataccgct agggatagta aatctattta gttggtatga ctttagaata
     3961 tcagttgcag ttaaagtatt tgcttgtcta tcaaacactt ctaatagaag tgagttcaaa
     4021 tctgattttg atagagctga taacttatca actatattct tatccccaat tttatttgta
     4081 attctactta aaatatcact catagttatc ctccatactg ttactttcaa aataattata
     4141 gaattgttac aacaactaat ctaggtcatt tgaccaaaaa tgaaaaaaca ctgaatattt
     4201 actattcaat atttttttac tttgggttta tttgattaaa tcttgaaaat actgtaatgc
     4261 aaccctttca gcttcaacct ttcggacaat aggactttgt gataattcct ctcttaactc
     4321 aagatacagg ttgatagtag cctgcaatgc attaacacag cttttatatt catggttagc
     4381 aaccgtcttt tctaatgctt caatatattg gggtgcgtcc aattcaattt ttctaactcc
     4441 acgaggtaat ttacctaaga gaagctgtat taatggggca agtactgatt gtcttaaaaa
     4501 agataaaaaa tcaattgttt caaatagttc tctacgtcca atcttagtgc aagcgtaatg
     4561 aacccaaatc caaaatctat cttcaatcca ctgtaagtct ggagttggga agtatgcttc
     4621 ttttttagaa taaatttctg ttatgcagtt gttttcttca tagagaatag ctgaatcttc
     4681 aaccctatct agtaaaccat ctaaggttgt aaacttaaaa tcaacatgaa gaacagggga
     4741 atcatagagg gaaattatca atcgaggttc tccaacgtgt tcgccagtaa aagcagaaac
     4801 taatgttcca aactgttcca caataagtaa tctctcttcg ataacttctt cataggattt
     4861 atcatctgct acaatcagaa aatccaaatc agaatattca tctaacgatt ttgtgatata
     4921 agaactgcta attgcaagtc ctattatcct actatctctt tttgaaaatt ctaatacgtt
     4981 ttgtatcatt ttctgttgtg ttttagtcat aatataacac ctctcactgt cttttattag
     5041 ctatcaatcc aacataatta tgcataggag tattatataa tgaagaaata aaaaaaaata
     5101 ggtcacttga cccgaaataa aaaagcattg aataattatt attcaatact tttttgaaat
     5161 tatacttatg ttgtaggaag attctttaat acctctcgct gattaattac actaaatctt
     5221 ttgttttcat aagcaattag atgttcttgt acacattcat ttatagcacg attaatgctt
     5281 ctaattggag cgtttacttc gctagcaagc tgttttttgg taagcagatt taattcactt
     5341 ctataatagt gtaaggatat acacctcaga agtcgttctt taacacttaa agatgataat
     5401 tcttcaatta attcagcatt tacaactaat ttactagata tttctcgaat cagaaatttt
     5461 gtagcttcaa aatctcccct taaccagttt aaaaaatcat ctctatgaag tttatataca
     5521 ttacaatctg tgaaagcgac aatttcaacg ggttttttcc catcattaaa ttgttccatt
     5581 tctccaaaaa aactaccact tctataaaga tgaatcgttg agatgtttcc tttcacattt
     5641 aagatgaatg cttctgcaat accgtcaatc ataagaatta catattcatt ttcttgctca
     5701 gcaaaaagaa tagtctcttt ctttttatac gtttttttaa caagcttctt tttaatatct
     5761 tcattcataa aatcaattaa atgcatattc atccctcctt accaaatctt tgattaaatt
     5821 atatcatctt tcaattagtt aggtaagata gtaaaaatat atttacatag aaactattcc
     5881 attattattt tccagttgtc atttttctgc aaaaacttat cactgtcacg actggagata
     5941 ccagtaagga atgttaggat atcaatcgct aaactttcag aatccttttt acggctcaaa
     6001 atgacgtaag ggtcaagcaa tcctttgttt tcatcattac tggttaaatt gacaatgttt
     6061 atctcatggg ctatttctgg tagcgtttct ttccatttgc cgcgttctgc tttagggtca
     6121 actatgaccg cctgtccgcc gaatagtacc gcatagtaaa caagcatatt gttactgaat
     6181 aatttaccac cacccaatga accgataaac gcagacgcta acgcattggt aatagaaccc
     6241 tttacccctt gactagcaag ggcaggtttt agatagacat tctttccagt gtccagattg
     6301 tagccaagat agatatcctc atgttcgcca agggtttgtg tcgctccaaa accaagtcca
     6361 gccaaaaaat ccgaagtgac atattgaata taatcattga tgtaacgttt gctgacagga
     6421 ataaattcgc catgcaatcc caacatatcc ccaaagggac gcacgagctt gattgataag
     6481 tcgtcgtaaa agtctttgac ttcatcacaa cgccttttta gctcgtcaag attctttgta
     6541 gatacacgga caacatagct aagtttatac atagattctt tacagttaaa agtctgtttt
     6601 taaaaaaatg tataaattat aaatcaaaat atagatacta tcaatgaccc actaggtgca
     6661 aaaagcattg atatggttaa attatcagac gctaaaagct gggctatccg aatgagtgaa
     6721 gatgggtgtg cttataaatc cattgacaat tataagcgct cactgaaagc gtcgtttttc
     6781 atggcaattc aagacgactg tattagaaag aatccattta atttttagtt aagtgatgtt
     6841 ttggaagata caacagaacc aaaaatcgct ctaacaccag aacaggaaat gggcttatta
     6901 tcctttatgg ggagcgataa gacttattgt aaatattatg atgatgttgt tatcctctta
     6961 gaaacaggac tacgcatttc tgaattatgc ggtttgactt tagaattgga tatgaaaaat
     7021 aaagccatta tggtagacca tcaattgttg agtgatacta agattggcta ttatgtagaa
     7081 gttccaaaga ccacacaggg taagcgagaa attccaatga ctgaaagagc ttatcaatca
     7141 tttgaacgta tcgtggaacg gcgtaagaac gctaaagcag aaccaatcat tgttgatggt
     7201 taccagaact tcctatttct taaacaagat ggattaccaa aacaagcaac tcattatcaa
     7261 agcatgttaa gagcgttagt caagaagtat aataagaccc atgaggacaa gctaccaaag
     7321 attacgccac atacctttag gcatacactc tgtacaaata tggctaacaa aggaatgagt
     7381 cctaatatat tgcaatatat tatggggcat aaaaacatta ctatgacgct tggctattat
     7441 gcacatggtt catttagttc ttcgagagct gaaatggaac ggttataatc ctagtagtat
     7501 gactagagtt tactacttat ttactacttt tgatggcatt cttatacggt taaatgccaa
     7561 cttatgcaaa gtatcttcca aaaagaaaat gccgatactc cttgtagata caggatatat
     7621 cgacatttac caacttatga aaagataatc aaatttaata taagtttttc aaataaaaag
     7681 ataccaaatt ctttttcgag aatctatata acattactta agcttggttg gggtataaac
     7741 aattgagtag ctacttttgt atggtgttca actttagtca aaactcttaa gtaatgcaaa
     7801 tgatttagat tcattaatat tcaatctctt cataagtagt gaaattcgag ttatttctgc
     7861 ataattatac tatgatttat agattaattc tattagcatc cagaataata ttgtttatat
     7921 gagtaaaatt taaagaatat tgtgaaaatt ataggatgtt aaaataaatt taggtaaaaa
     7981 agaagtgttt gtatattata gagtaccttt tctaaaatat gtgggattgt tttaagaatt
     8041 agaataaaaa ttatttttaa attagtattt ttataatgta tagtattttt aatgtataat
     8101 taaattgtgc agaaaataga atttaaagtc ctatagaatt tctgattatg gaggagaata
     8161 tgaaaagaaa agttcaagta aaaaatatta ctattggaga aggtagacct aaaatttgtg
     8221 taccaattat tgggaaaaat aaaaaagata ttataaaaga ggctaaagag ttaaaagatg
     8281 catgtttgga tattatagag tggcgagttg atttttttga gaatgtagaa aatattaaag
     8341 aagttaagga agttctctat gaattaagaa gttatataca tgatatccct ttattattca
     8401 catttagaag tgtagtagaa gggggagaaa aattaatatc aagagattat tatactactt
     8461 tgaataaaga aatttctaat acaggtttag tagatttaat agatgtagag ttatttatgg
     8521 gagatgaggt catagatgaa gtagttaatt ttgctcataa aaaagaagtg aaagtaatta
     8581 tatctaatca tgattttaat aaaactccaa aaaaagaaga gattgtatct cgtttatgta
     8641 gaatgcagga attgggcgca gatttaccca aaatagctgt tatgccgcaa aatgaaaaag
     8701 atgtgttagt gctacttgaa gctacaaatg aaatgtttaa aatttatgca gatagaccaa
     8761 taataacaat gtcaatgtca ggaatgggtg ttataagtag attgtgtggt gaaatatttg
     8821 gttctgcatt gacttttgga gctgcaaaaa gtgtttcagc accagggcaa atatcattta
     8881 aagagttaaa ttcagtactt aatctattac ataagagtat taattaaatt aattatatag
     8941 aaatattaga cggtattttt gaagatataa attaaatata aaaaactttt cagatagtaa
     9001 aatttaaatt gggcaattat atctgaaaag caactattta gtgtgtattt tgaaagtgat
     9061 atagaaaatc ttataaatca actatggcat atttaaaata tgccattaaa acctactaga
     9121 cattagatta tagatggtat actagcattt ctattattta tagatagtaa gttttcataa
     9181 taactgtttt atctaaagta gaagatatat ttgtattatg tatatttgtc attatttttt
     9241 tgtaattatg tatattaatg gttatatatg taatataata ttatcactct attttttgta
     9301 tattgtacat tacaacattt atatctatca tcatcaatta cagtagaatg tatgggatac
     9361 ttattttcac ttaaccaata tttttgcata ttctttgtcc aagactttgg aaacttcaat
     9421 actttcttgt ttattataac atcctcatct tcaaaaatat taacttctct ttctattttt
     9481 atttttctgt tgtatatctt attgatagat tctttgtcga gttttatgtc tctatacata
     9541 tcagggcaat agcaacaaac ataatttctt ttttttcgac gtttaaattt cacaactttc
     9601 tctatgctaa tttctaattt ttttgtacca tcttttataa atttaatatc ttctatgttc
     9661 tcgcttgaaa aaatttctgt caatcttaaa gtatctacag tttctctaat tatttgcttt
     9721 ttataagaag agaatccata tccaaaagaa aggtttaatt tattagggat gtagccattt
     9781 ttatatttaa acatcttaat tttttccaat tcagcattat ctttaaattt caacttttca
     9841 tacctaaaag ttactttgct aaaaagagta tattctacat caatatattc ttttgcaatc
     9901 ttaaatttat cgctaccttc aatcacaatc atagcctatc tccaatcgtt ttatattaaa
     9961 ataaggccat agaacatggt ctaatagtac tcattagcac tataagacaa tgttaacaca
    10021 aaaagacaaa aaaaggttat ttatttcaaa taaccttttt ttttatataa aatataaaga
    10081 attatatttt attctttttt atgatgtcta taatctgtga tatcagattt agttctcttg
    10141 aatttaagtc ttttaactct tcatttatat cattttctaa atatgttttt ccagtggaag
    10201 taatatcttt tataagaaca tctgcagaaa tatttaatat atttacaaat tttatgaatg
    10261 ttttaagact tggattcttt ttaccacttt cgatttgagt tatataccaa ggcgatactc
    10321 caaccatatt tgcaaattct tttgaagtta aatttgcttt tttccgttct tctttaagtc
    10381 ttttacctat ataattcatt attgttacta tttccctcac ttatatttct ttgtatttaa
    10441 tgatgcactt tattaactct agcattgcaa tttgttcttt ttcagttaat ttattaatca
    10501 tttcataaca ttctaaaggt acagagttat caacgccttc tgttgtaata atatttgcaa
    10561 ataacaattc aagtggtatg tctaaagcag tagcaatttt cattataata ttgatagatg
    10621 tatttgtctc agctctttca atctgaccaa tatatgttgg atgtaaacct gccttttctg
    10681 ctagaaactc ttgactatat cccaattttg ttctatagac ttttattcta tctcctatta
    10741 tttttcctat atttgccaat tattatccct ccactttaga ttatattcat agtttaagtt
    10801 gttttatata gactatttat acttttttag tctaaatata gactatatat gtttttatac
    10861 tgaaataagt gctatttatt tttgtatagt ctgtatctac aataaacaaa tatggataaa
    10921 tcatattttt aattagagaa atattttcat aatttcatag ccatactaca ataataaaac
    10981 gaattaataa tatgtt
//
LOCUS       DAYASV010000123        10941 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYASV010000123 DAYASV010000000
VERSION     DAYASV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10941
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.126"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            255..719
                     /locus_tag="ACGI68_003014"
     CDS             255..719
                     /locus_tag="ACGI68_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFH0139436.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            726..1220
                     /locus_tag="ACGI68_003015"
     CDS             726..1220
                     /locus_tag="ACGI68_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HFH0139437.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1236..2261
                     /locus_tag="ACGI68_003016"
     CDS             1236..2261
                     /locus_tag="ACGI68_003016"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HFH0139438.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2248..4695
                     /locus_tag="ACGI68_003017"
     CDS             2248..4695
                     /locus_tag="ACGI68_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFH0139439.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNKVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4875..6248
                     /gene="radA"
                     /locus_tag="ACGI68_003018"
     CDS             4875..6248
                     /gene="radA"
                     /locus_tag="ACGI68_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFH0139440.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSEKLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6251..7321
                     /gene="disA"
                     /locus_tag="ACGI68_003019"
     CDS             6251..7321
                     /gene="disA"
                     /locus_tag="ACGI68_003019"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HFH0139441.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7410..8504
                     /locus_tag="ACGI68_003020"
     CDS             7410..8504
                     /locus_tag="ACGI68_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFH0139442.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8633..10810
                     /locus_tag="ACGI68_003021"
     CDS             8633..10810
                     /locus_tag="ACGI68_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139443.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TVYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
BASE COUNT         4584 a         1102 c         2018 g         3237 t
ORIGIN      
        1 taataaagat gagaaggtat atttaaaata taaagtattt aaagttctga aatctaatca
       61 tgaagcaaaa aattagcatt tggttatcaa taaatataat aaataaaaac tcataattaa
      121 tgaaagaata aattcatatc taaatttcta taaaaaattt aaatttatac aatttagggc
      181 tttaaaaatt taaataatag gtgtaaaata atagtcaaag aaagtcaaaa ataaaaaggt
      241 gtgtggtgat attaatggca actatgacag atataattga aaaatttatt aaagatttga
      301 tggaagaaga taatagcata caaattcaaa gaaatgaact agcaaacttg tttagttgtg
      361 caccatcaca aataaattat gtcttaacaa caagatttac aatagataga ggatattaca
      421 ttgaaagtaa aaaaggtggc ggaggatatg ttcaaataga aaaaatacga aaaagtaaag
      481 atgggcatat tagagaacta ctaaatgaaa aaataggaag tcagatatcc tataaaaaag
      541 caaaagaatt attagaaggt ttaaaagagt cggatttgat taacgaaaga gaattaaaat
      601 taatattata tgcaatagat gataaatcat tgtgtatgcc aatatatgaa ttaaaagaga
      661 aggtaaggtc taatatttta aagaatataa taataggatt atttagtata gaggagtgat
      721 aatttatgct atgtcaaaaa tgcaataaga ataaagcatc tgtatattat aataagatag
      781 tcaatggaga aaaaacagag atgtatttat gtagtgagtg tgcaaaagag aatacagaaa
      841 tgaattttaa cttagatatg cctttttcaa tgatggatat attttctaat ttaggttttc
      901 aacctaaaaa agaacttgag gaaaagctag tttgtcctaa atgcaataca acatatagtg
      961 agtttaagaa taatgggaga tttggatgta gtgaatgtta taatgcattt agtagtcaag
     1021 taaatccaat gcttcaaaat atacatgggc atatagaaca tacaggaaaa gcacctaaaa
     1081 aatcttttta taagataagt gtagaaaatg agattaaaga actaaaagaa gatttagata
     1141 gagctataaa aaatgaagag tatgagttag cagctcaatt tagagataaa attaaatatt
     1201 taaaaggttc tattgactaa gattggagtg atattatgaa agagaacata gtcatgaaat
     1261 caagagttag gctagctaga aatttaaata actatccatt tccaaataaa ttagataaag
     1321 aatgtgccat ggaaataata gaaaaagtaa aaaatgcgtt tattaattct aacttagaac
     1381 aaaaagaaga atttgatttc tataaaattg aagatttaga ccaatctaaa aaaatgctta
     1441 tggttgaaga acatataata agcccagatt tagcagaaaa tgataaaagt gcagttatag
     1501 taaaaaaaga taaaactata agtataatga taaatgaaga agaccatata aggatacaaa
     1561 ctatatgtga tgatttaaac ttagaatatg catatagtgt agcaaatgaa atagatgatt
     1621 tgttagaatc aagtttagaa tatgccttta acacaaagct aggttatcta acatcttgtc
     1681 caactaatac aggaacagga atgagagctt ctgtaatgat gcatttacca gcattaagtc
     1741 aactaggcta tatggatgaa ctttataaaa tttcatcaca aataggtata gcaattagag
     1801 ggatatatgg agaacgaaca gaagcgttgg gaaatatata tcaaatatct aatcaactta
     1861 cacttggaag aacagagtct aatattatag aaaatgtaag tggacttaca aaagatgcaa
     1921 tttcaaaaga aataaaagct agagaaatat tgcaaaaaaa attaggtata aaactagaag
     1981 ataaaatatt tagatcgata gggacacttg aaaattcaag agtaatgagt agcgcagagg
     2041 caatgagcca tctatcaaat ataaaaatgg gaatagaaat gaactatata gataaactag
     2101 atttaagggc tatagaacaa cttatgatag gtatacaacc agcgcatcag tcaataatgt
     2161 ataagagtga tgatgttgag aatagagata taaatagagc aacatatatt agagaaactt
     2221 tggaaaaatt aagaggaggg atgaattgtg aacttcaata gatttacaca aagagctaaa
     2281 aaggcaattg atttagcgtt tgaatctgct aaaagtctag gacacaatat tgtcggaagt
     2341 gaacatatac ttttaggtct tttaagagaa gaagaaggta tagctgctaa agttctaagt
     2401 aaagttggat ttacagaagc ttatttagaa ggcaaaatag ttgatatgga aggtaaggga
     2461 gaagaaatct cagaagatat agtattaagt ccaaggagta aacaaatact tgaattatca
     2521 ggaatgtttg caaataaatt aaaaacaaat tatattggga ctgagcatat attattagca
     2581 attattcaag aaggtgaagg aatagctaat aagattttaa attatgctgg agtaaatgat
     2641 aggactttag cccaattaac aattgatatg atgggtatta gtgataaaaa tcaatacaaa
     2701 gcagagaata gttacacggg caaccagaat caagcagaat ctaaagtact tgataagtat
     2761 ggaagaaatc ttacactata tgcaaaacag aataaaatag accctgtaat aggtagagaa
     2821 aaagaaatac aaagagtaat acaaatatta agtagaagaa ctaaaaacaa tccagtgttg
     2881 ataggagacc caggtgtagg gaaaacagcc atagctgaag gtctggctac aaatatagcc
     2941 ttaggaaatg ttccagaaac actaaaaaac aaaactttat actctttaga gatgggttca
     3001 ttattggctg gagctaagta tagaggagaa tttgaagaaa gaattaaaga agttgtagat
     3061 gaagttgtta aaaatggaaa cataatctta tttatagatg agatgcacac aataataggg
     3121 gctgggtcta ctggagaagg ctctatagat gcttcaaata tattaaagcc agccttagca
     3181 agaggagaaa tacaagttat aggtgcaact acaattgatg aatatagaaa gcatgttgaa
     3241 aaagattctg ctctagaaag aagattccaa ccagttatgg tagatgaacc aactaaagag
     3301 gactcaataa aaatattaga aggattaaga gataaatatg aagctcatca taaagttaaa
     3361 ataactgatg atgctataaa ggcagctgta gaattatcaa ctagatatat atcagacaga
     3421 tatctgccag ataaagctat agacttgatt gatgaagctg catcaaaagt aagattaaaa
     3481 gaaaatacac ctccttcaga aataaaaaaa ttagaactag aaatagaaaa tatagataaa
     3541 gaaaaagaag aagcagtaag atgtcaagac tttgagaaag cagccaaaat aagagatgaa
     3601 caaggtctac ttaaaaaaca attagaagat gttagagaaa gatggaacaa gtcatctaaa
     3661 cattcagatt tagttgatgg agaagttatt gctgaagtag tgggcttatg gacaggaata
     3721 ccagttaata aaattcttga agaagaggct gatagacttt tgagacttga ggaaatactg
     3781 cacaataaag ttataggtca agaacaagca gtaaaatcta tttcaaaggc aatcagaagg
     3841 tcaagagcag gtcttaaaga ccctaataga ccaattggtt catttttatt tttaggtcca
     3901 acaggagtag gtaaaacaga attatctaag gcattagcag aagtgcaatt tggagatgaa
     3961 aatcaaataa ttagaataga tatgtctgaa tatatggaaa aacatgctgt gtcaagaatg
     4021 ataggttcac ctccaggtta tgtaggtcat gatgaaggtg gtcaattaac tgaaaaagta
     4081 agaagaaatc catattcagt tatcttattt gatgagatag aaaaagctca tccagatgta
     4141 tttaatattc tgttacaaat tctagatgat ggtagactaa ctgattcaaa aggaagaact
     4201 gtagatttta agaatactat tgtaataatg acatcaaatg ttggagcatc tacaattggt
     4261 agacaaaaaa ctttaggatt tagtatagct aaaggagacg aagaagaaaa atctcaatat
     4321 gaaaaaatga aagaaaatat aatgggtgaa ttaaaacaaa gattcagacc agagttttta
     4381 aacagaatag atgatataat tgttttccac tcactaaatg aaaatcatat atcaaaaata
     4441 gtacttttaa tggcagcgaa actacaagag agattaaaag aaatggatat aaaattagaa
     4501 atgagtgatg aggctgttaa attaatttct aaatctggat ttgacttaga atatggagca
     4561 agacctctta aaagagcttt acaaaaagag ttggaagatg agctatctga agcaatctta
     4621 aaaggtaatg tgaaaaaggg aagtaatgta gtagcaaaag tcaaagatga aaaaatagtt
     4681 tttgaaacta aataatttaa tagagttgca ttttaaaata ttgcaaatat aagttttata
     4741 atctaaacaa aattttaagg gcaatgaatc tataataatt cattgtcctt aattatttta
     4801 tttaatcttt acatattgtc tttcagatat gctaaaatta tagtatagaa taaaaaatta
     4861 aggatgataa aagtatggca aaaataaaaa caaaatatgt atgtcaatct tgcggatatg
     4921 aaacagctaa atggcttggt aaatgtccag aatgtacaaa atggaataca tttgtagaag
     4981 aaatagacca aaaaagcacc aaaaaagagg tctttataat agataaatct tcatctaaac
     5041 cagtcagtat aaactcaata gagagtaaag aagaagagcg gtttacaaca gatataaatg
     5101 aattagatag agtgcttgga ggaggtatag ttaaaggctc tttagtgctt gttggtggtg
     5161 accctggtat aggtaaatca acacttttaa tccaagtatc tagtaatgtt gccaatttag
     5221 gaaaaactgt tttatatata actggagaag aatcagaatc tcaaataaaa atgagagcta
     5281 aaagattagg tattaactca gaaaaattat atatatttgc tgaaaataat ttaagtataa
     5341 ttgaatctta tttagaaagt gtaaatccag aattaataat tctagactca attcaaactg
     5401 tcttcagtcc agaaatatcc tcggcaccag gaactgtcag ccaaattaaa gaaggtactt
     5461 caaaattcat gaaaatttct aaaaaaatgg gaatttcaac gttcatagtt gggcatgtta
     5521 caaaagaagg ttcactagca ggaccaaaat tattagagca tatggttgat actgttctat
     5581 attttgaagg agaaagatat aatacttata gattagtaag agctgttaaa aatcgatttg
     5641 gttcaactaa tgaacttgga gtatttgaaa tgagggattt aggattagta gaacttgata
     5701 atccatctaa aattttaatc tctgaaaaac caaaagatgt agcaggttca gtaataatat
     5761 ctactgtaga aggtacgagg ccaatgttac ttgaattgca agcattagtt tctcctacaa
     5821 gttttggtat accaaaaaga acatctacag gagtcgatta caatagagta ggtatgttat
     5881 tagcagtatt agaaaaacgt gttggtttgc aaatacaaaa tcaagatgtc tatataaaca
     5941 tagttggagg aataaagata aatgaaccct caattgattt agggatagcc atttctgtag
     6001 catctagttt tagaaatata ccgatagatg aagatatagc tgttacagga gaagtgggac
     6061 ttacaggaga agttagagca gtaagcttta tagaaaagag aatagctgag tgtaaaaaac
     6121 ttggatttaa aaagatagta gttcctagaa gtaattatga tgttgtaaaa gagaccaaag
     6181 gcatagaaat atggccagta gataacttaa gacaagctat aaatatagta cttgggagga
     6241 atcaataaat atggagaatt ttctagataa taaaaatatg ctatatgcat taaaaatgat
     6301 atctcctgga actccactta gattaggtct aaacaatgta ctaagagcta agactggtgg
     6361 attaattgta attgcaacaa acgaagatgt aatgaaaata gtagatggag gatttgctat
     6421 aaatgcagaa tattcaccat catatctata tgaattagct aaaatggatg gagctatagt
     6481 tttaagtggt gatgtaaaga aaatattatt tgctaatgca caacttatac ctgactattt
     6541 tatagaaaca tcagagacag gaacaagaca tagaacagca gaaagagtag caaaacaaac
     6601 tggtgctata gtcataggaa tttcacaaag aagaaatgtt ataacagttt atagaggaaa
     6661 tgagaagtat gtagtcgaag atatatctaa gatatttact aaggcaaatc aggctataca
     6721 aactctggaa aaatataaga cagtattgga ccaagctgta acaaatttaa atgccttaga
     6781 gtttaatgat ttggtaacta tttatgatgt tgcattagtc atgcaaaaga tggaaatggt
     6841 aatgagagtt acaagtataa ttgaaaaata tgtgatagaa ttgggtgatg aaggaacttt
     6901 agtaagtatg caattagaag aattaatggg tacaaccaga atagaccaga aattaatatt
     6961 caaagattat aataaagaaa acacagaaat aaaagaactt atgaaaaagg tcaaaaattt
     7021 aaattcagaa gaactaatag aattggtcaa tatggcaaaa ctattagggt atagtggttt
     7081 ttcagaaagt atggatatgc ctataaaaac aagaggttat aggattctta gcaaaataca
     7141 tagactacca acagcaataa tagaaaactt agtaaattat tttgaaaact ttcaacaaat
     7201 tttagatgca tctattgaag aattagatga ggttgaagga ataggtgaaa taagagcaac
     7261 atatataaaa aatggactca taaaaatgaa acaattagtc ttattagata gacacatatg
     7321 atgaatttta aatgaatttt actacttttt tcttgaatat aatatacact ttggtctata
     7381 atagataagt ataactaggg ggtgaagcat tgataaggaa agtaacaaga atattgttta
     7441 ttttacttgg atttacaata ggtataacaa cctatttaac attaatgaaa gattttgaaa
     7501 tattaacatt tggaaaagaa acatatggtt atatcgcagc tgtagtagca ggtataataa
     7561 tagccatttt aggatatctt atagagccat gggttgtaaa taaagtaaaa gaaattgcta
     7621 aaatagtgga taaagaattg tcaaaatatc ctcagactga tatattgtta gggtctatgg
     7681 gtcttatagt aggatttgtt attgcgtatt tattaagtgg attagtaaat agaataccaa
     7741 tagtaggtgg aatactatcc ttactattat atttattcct aggatatctt ggaatgaaag
     7801 ttgcgcttaa aagtaaaaat gatttattta atattggaaa actaggaaga ttagcaaatc
     7861 ctattaagga taaagataag gataaagaaa ataaaaaaga agtaaaagcc attcctccaa
     7921 aagtattaga tacaagtgta ataattgatg gaagaatagc tgatatttgt aagactgggt
     7981 ttatagaagg aaaacttatt attcctgctt ttgttttgga ggaactaaga catattgcag
     8041 attcttcaga tgatttaaaa agagtaagag gtagaagagg tctggatata ttaaatataa
     8101 ttcagaaaga attaaatata gaagttgaaa taagtgagag agactttgat gatatagcag
     8161 aagttgatag taaattatta aagcttgctc aggttttaaa tggaaaagtt gttacaaatg
     8221 attataactt aaataaagtt gctcaattcc aaggtgttga agtattaaat ataaatgagt
     8281 tagctaatgc tataaaacca gttgctatac caggtgaaga tatggttgtt caagtggtta
     8341 aagaaggaaa agaggctcaa caaggtgttg catatctaga tgatggaact atgatagttg
     8401 ttgatggagg aagaaaatat atgaatgaga ctataaaagt actcgttact tctgttcttc
     8461 aaactcctgc aggtagaatg atatttgcaa aacctaaaaa ttaatatgtt caaagaaacc
     8521 tataatttgg gctgtttatt ttaaatagat ggtccaaatt tatttattta taacgataat
     8581 gtttaatgta ttcttaaaga tggggattta aatcagaaag cagggagaat ttatgagaaa
     8641 aaagattata tctttcttag caacatttat cataatatta acatcagcaa gtcagtatag
     8701 ttttgcagat gatatatcaa cacgtggaaa agtaatattt atagatatga atagaactag
     8761 tatgagtaat atgttaagaa taaaatcatt aagagaagaa ctagacaata gagggtatat
     8821 tggcttgatg aatataagag gcgataaagg aagtgatgat agaaggtcat atgctagtat
     8881 gggtgctggt ggtagagcta atgtggctaa tgaagaagat attaattttg aaagctcgag
     8941 taaggataga aatatagttt ttgaatcagc tacaggaaaa agtgcaaaag gtataaataa
     9001 tttaactata aataagtcta taaatgaaaa tttaaacttt ggagagtatg gttctgtatt
     9061 agggtcatta ggacaaagtt tatctgataa tggattaaaa gcatctgtat tgggtaattc
     9121 agatataatt gaaaatggtc aacttataaa aaatagaaac ttatgtctaa ctgcaatgga
     9181 tgaatatgga agaataccta atggaaatgt agatactata aataaaaaag acttatctat
     9241 gccttatgga atatcaactg attatgataa attgattgta gagactaaag aagcttacaa
     9301 aaataatgat gtgatttttg tggagcttgg agacacatat agattagatt tatataaacc
     9361 aaatttaaat gaaaaaacat atgagagtat gaaagataac atagaaaaaa atatagatat
     9421 atatttaaaa aatatatttt ctatggttga agaaaatgac actgtctaca tagcaagtgc
     9481 atttccaagt gatttagatt ataaaaataa aagaagactt tcgccaataa taaaattaaa
     9541 tggagaaggg aaaggtatat tatcatcttc aactactaga agagaaggta ttgtaacaaa
     9601 tttagatgtt ggggttgaaa tattagacaa ctttaatata aaaaatcaaa atatggttgg
     9661 aagaaaatat gaacttatta acagagatga taataaagaa tttttgatgg atgaatatca
     9721 aaagatagta tctatttcaa gcataaggtc tacaatactg aatggttttg taagtatagt
     9781 tttcttgtca tggatagttg ctatgattgc aattttattt agaaagcata tatcaaaaaa
     9841 ttataaagaa acaacatttt ttatattaaa ggaactttta aaaataggaa tagtaatgcc
     9901 actgtcattt atgataacac caataatgaa tttcaaaaca ccattagcaa tatctttagg
     9961 aataattata ataacactta ctatatatct tataagtaaa gttttaataa agaacgattt
    10021 aaaaaacatg ctgtttttta ctggattaac catagtaatt atggttattg atgctgggtt
    10081 tggctcatat ttaatgaaaa gcaatgttat gagttatgat tgtataattg gagctagata
    10141 ttatggagta ggaaatgagt atcaaggtgt ggcaatcggt agtgcaatat ttacatttgc
    10201 aattttactt acatataaaa atataccaaa atggtctata atagtttttt ctttggttat
    10261 cttaattaca agtgcatctc caattatggg agcaaatgtt ggaagtgcta tttctgaatg
    10321 tgttgcattt ttactattta tactattgat atataatgta aagatagatt tcaaaaaaat
    10381 cgtattatta ggtatagcag tagtatttgt attaggagtt tttgtagcaa tagatatgat
    10441 tttgggaagt aattcacatt taggcatgtt tgtaaaagaa atttacttta atggaccagg
    10501 agaaataata caaacattta gtagaaagat agagatgaat ttaaagctag ctcaaaccag
    10561 tgcatgggta aatatactgt taactggaat aggtgtaatt ttagttctta tgattaatca
    10621 gattagatac tttaagcaac tgatggatga gtatccaatt gtatttaaag gatttatagc
    10681 atcaatagca ggatgtttag taactctttt agtgaatgac tctgggattg tgtcatcagc
    10741 tacagccttt atttatgtaa ttgtacctat gataacatta agtataaatc ttacagcatt
    10801 aaaagaataa gtactgtatg aataatatgt aaatattaat acattaattt aacttcttta
    10861 tttacaaaat aaagaagaaa gtttataaaa aataaattat aataaaaaag agtttatctc
    10921 aaaattgatt attctaatca a
//
LOCUS       DAYASV010000124        10838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYASV010000124 DAYASV010000000
VERSION     DAYASV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10838
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.127"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            280..1464
                     /locus_tag="ACGI68_003022"
     CDS             280..1464
                     /locus_tag="ACGI68_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139444.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
     gene            complement(1841..2968)
                     /locus_tag="ACGI68_003023"
     CDS             complement(1841..2968)
                     /locus_tag="ACGI68_003023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0139445.1"
                     /translation="MKGKITMDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEG
                     KDVIAEAQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESK
                     DVSILAAYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQ
                     MLLMGFKNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLN
                     NIKQEVVETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLH
                     SDIAQSKRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIG
                     RTGRAGEEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            3413..4606
                     /gene="nifV"
                     /locus_tag="ACGI68_003024"
     CDS             3413..4606
                     /gene="nifV"
                     /locus_tag="ACGI68_003024"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HFH0139446.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            4631..6556
                     /locus_tag="ACGI68_003025"
     CDS             4631..6556
                     /locus_tag="ACGI68_003025"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HFH0139447.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWVELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            6578..7573
                     /locus_tag="ACGI68_003026"
     CDS             6578..7573
                     /locus_tag="ACGI68_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HFH0139448.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            7898..8371
                     /locus_tag="ACGI68_003027"
     CDS             7898..8371
                     /locus_tag="ACGI68_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0139449.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            8405..8899
                     /locus_tag="ACGI68_003028"
     CDS             8405..8899
                     /locus_tag="ACGI68_003028"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139450.1"
                     /translation="MIRKANMNDLESIMKIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            8996..9517
                     /locus_tag="ACGI68_003029"
     CDS             8996..9517
                     /locus_tag="ACGI68_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFH0139451.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            9706..10017
                     /locus_tag="ACGI68_003030"
     CDS             9706..10017
                     /locus_tag="ACGI68_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0139452.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            10045..10731
                     /locus_tag="ACGI68_003031"
     CDS             10045..10731
                     /locus_tag="ACGI68_003031"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HFH0139453.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         4163 a         1173 c         1948 g         3554 t
ORIGIN      
        1 tataataata aagaaaaaat tatataataa taaagaaaaa atgtataata tttaacatta
       61 aaaaagatat ttcataaaat taaaaaacaa agtgaatttg aatactattt aaaaaataaa
      121 ataaatatat aaaaaatatt taatataaag gtggaaataa gttgaaaatg taaagtcatt
      181 ggaaaaatat agttgaattt aatgagcaaa aatgttattg ttttatcaat aaagtgttaa
      241 aataaaaact ttcaatatca ttttagggag gatgatttca tgcaaaaaag tgtaaagaaa
      301 gcaaaagtaa cgggctctat gttagctatt tttggtgttg caagcgttct ttttagttca
      361 catgcaggag gaggatttgc tacagggaat caagaaactc agtactatgt gcagtatgga
      421 tggacagcac cacttatggc aattttagca atgattatac ttactgctac catgagagaa
      481 gttatcataa tgtataataa taacaattgt aggaattata aggatttatt ttgtgagctt
      541 tggagaccat atccaaaact tgaaataata tgggagatat attactatct aatggtttta
      601 atagctgtaa gtgctgttat agcaggtgca gcagcagtat ttcaaagtat aggagtaaat
      661 tattttgtag ctgtatttat tattggagtt gtacttcttg tctttactat atttggtgct
      721 atgcttgtat ctaaagcagc tacagcaatg acaatagcta ttctagtatg tactcttact
      781 atttttattg ttggtataaa agctaaagta ccagaaataa ctgaaatatt atcaaataga
      841 acgagtttca cacctggcta tatcaagcca atacttaata cttttattta tgcaggtttt
      901 caatcggttg ttatacctac acttgcagga tgttcaagac cacttcttaa gaactcaaaa
      961 gaagctacta aggcaatgat aatttcattt gttatgaatg caattgcatt aggacttgct
     1021 gtaactatgc taatgggatg gtatagagaa attattgctg ctgggcaaac aacactccct
     1081 actctttatg ttgcaggtca atctggaaat catactatat acataattta taatgttgca
     1141 ttattccttt gtttaatgtc tacaggagta actactatat ttggtcttgt aaatagattt
     1201 gaagaccata aagcactttc tttcctttct tcaagaatga aaagaagggt atttacagct
     1261 tgtgcaataa tggtagtgtc aatgcttata tcacttacag gattaagtaa tattgttaaa
     1321 tatggatatg gatattgtgg ttatcttggt ctattcacta tagtaattcc atttcttaca
     1381 ctaggacatt ataagaataa gaagtttgca aaggaaaatc cagaagctaa atggccagcg
     1441 gaattaaatg aaaatgtaaa ttagatgtag tagcaaaaaa tattgctaaa gagaatacta
     1501 taaataagaa tactatagat aatatatctg catgtttatt aagattattt gcaaaatcta
     1561 atattaaaca actataaaca agcaatataa aactaagaat aaaagataaa aagagggtat
     1621 ctcaaaatgg acttttagtt tataaggcac tcttttttat atgaagtcaa atatattttc
     1681 attattttga caataaaaga gactaatcct actttggata gcctcttttt tctttaatat
     1741 aaaatagtat aacagcaaag tatagattga tacatgtttt ctgtagcttt ctatatatta
     1801 cagtgtttgt gtggcattgg tattttaagc aatctataaa ttataattct ataattcttc
     1861 tactaatttt agattctata gccgtctcta tttcacttaa cattcgttca tttttagggt
     1921 caataaaagc acatgtatag ccttcttcac cagctctacc agttctacca atacggtgga
     1981 tgtagtcttc tggtgtttct ggtaaatcgt aattatatat atgacttact ccagatatat
     2041 caagaccacg agaagctaca tctgtagcta ttaagtattg tatatctaaa tttctaaatg
     2101 atttcattat tctttctcgt tttgattgag caatatcact atgaagtttt tgacaattat
     2161 atcctcttat agcaagagct tcttcaagat tgtcaaccct tctcttagtt ctacaaaata
     2221 ttattgccat aaaaggatta tcttcatcta gtaccttaca taaggcatct aatttttttc
     2281 tatctgtagt ttctactact tcttgtttta tattattaag agtgatttct tctttttgaa
     2341 tagaaactac aacaggattt tttgtgtatc tataagctaa tttttttact tgagaattta
     2401 tagttgcaga aaagcatagt gtttgctttt tgtttgaagt ctcttttaaa atagcttcca
     2461 cttcattttt aaatcccata agcaacattt ggtctgcttc atctaaaaca aaagtcttta
     2521 acttactaag gtctattgtt tttctattta gatggtctaa aagcctacct ggtgtagcta
     2581 taatcatatg aatatttcct tttagtttct ttatttgaga accaatatct tttcctccat
     2641 atgctgcaag aatacttaca tctttagatt ctttaagttt catagcttct tctgttattt
     2701 gaatagctag ttctcttgta ggagaaagta taagtacttg tatatcattt atatctaatg
     2761 atatattttc aaatatcgga agtaaaaaag ctagtgtttt tcctgttcca gtttgagctt
     2821 ctgctattac atcttttcct tcttttatta gtttaatact ttctttttgt ataggtgtag
     2881 gtgttgttat tcctgatttt tttagaatat ttattgtatt ttcactaatc cctaagcttt
     2941 tgaaatccat tgttatctta cctttcatga aaaattttct tttttattaa ccaattaata
     3001 atatcatatt tgtaagtatt tttagtaaat tttttaatta ttatttatat agcttaaaaa
     3061 tatattaaga tactttaaaa tggaatgtaa cttaaattaa attttagtag gaattttata
     3121 ggtttatttt taaaggtttt ttgaaaataa atctatttaa aaattaaatt aaaatgcaag
     3181 aaaaacataa taactttcat ttttaagaag ttttcacaaa aaagaaaaaa tatagtgcaa
     3241 taaatgatac aaagtaatat aatagattac aagatatgaa aagctaaaaa aataaattgc
     3301 aaatgaaatt aaaatgaata aaaaataaaa aaataatcat aagttagaag tatcaatgaa
     3361 aaataagact taatgttttt aaaattaaaa gtatactaag gggggcatta atatgtgcgt
     3421 tatatcaaaa gatagagcaa aggaaataaa aatagtagat actactttaa gagatggaga
     3481 gcaaacagct ggggttgtat ttgcaaatag agaaaaaata atgattgctg aaatgttaag
     3541 tgacctaggt gttgaccaaa ttgaagttgg tatacctact atgggtggag atgaaaaaaa
     3601 tgtaataaag cacatatgta gtagaaattt aaaatcagat ataatggctt ggaatagagc
     3661 agtaataaaa gatgttgaag aatctatttc ttgtggtgtt gatgcagtag ctatatcaat
     3721 atcagtatct gacattcata tagaaaataa attgagaact tctagaggct gggttttaga
     3781 aaatatggct aaaacagttg aatttgcaaa gaaaaatgga ttatatgttt cagtaaatgg
     3841 agaagatgct tcaagagcag atatagactt cttaactgaa tttataaatg ttggaaaaca
     3901 agcaggggca gatagattta gatactgtga tacagtagga gttatgaatc ctttttctat
     3961 aaagaatgca atagaaactc tttatgaaag aactaatttt gatatagaaa tgcatacaca
     4021 taatgacttt gggatggcaa ctgcaaatgc acttgcagga atagcagcag gagcaaatta
     4081 tgttggtgtt acagtaaatg gattaggtga aagagcagga aatgcagcat tagaagaagt
     4141 tttaatggca cttaagtgtg tttataaatg tgatttaaat aatatagata ctagaaaatt
     4201 tagaggaata tgtgagtatg tagcacaggc atcaggaaga atacttccta catggaaacc
     4261 agtagttggg gataatatgt ttatacatga atctggtata catgcagatg gagctttaaa
     4321 agacccacat aactatgaac catttgaccc aagtgaagta aatcttgaaa gaaaaatagt
     4381 aataggtaaa cattcaggaa gagctgcagt tgtaaataag ttgagtgaat atgaaatgta
     4441 tatttctcca gaaaacgcta ccaagctatt aaatgctata agagctacat ctataagatt
     4501 aaaaagaagt ttaatggata aagaaattct tcaattatat tgtgatatac tagcacatga
     4561 gaaaggcaca acagaagaag aagcagttag aggttcatat atttaagata taattgttta
     4621 ggaggaaaat atgggagata atatagttta taaaataatt aaaaaacata tagtagatgg
     4681 agaagctgtt gctggaagtt caattggtat aaaaatagac caaactttaa cacaagattc
     4741 tactggaaca atgacatatc ttcaactaga agctatggga atagataaag taaagactaa
     4801 aagaagtgtg gcatttgtag accataatat gttacaacaa ggatttgaaa atgcagacga
     4861 ccataagtat atacaaacag tggcagataa atatggagtt tatttctcta agccaggaaa
     4921 tggtatttgt catcaagtat ttttagaaag attttcaaca ccaggagata cacttttagg
     4981 ttctgatagc cacacaccta ctgctggagg tgtgggcatg atggctattg gagctggtgg
     5041 attagatgtt gctttagcca tggcaggagg agcatactat ataaaagcac caaaagtctg
     5101 taaggtgaat ctagttggaa aattaaataa tatggtatca tcaaaagata taatattaga
     5161 agtgttaaga aaacaaactg taaaaggtgg agtaggtaaa gtttatgaat atggtggaga
     5221 aggtgtcaaa agcctatctg ttccacaaag agctacaata acaaatatgg gggctgaact
     5281 tggagcaact acttcaatct tcccaagtga tgaaaaaaca ctagaatttt ttaagagtca
     5341 aggaagagaa gacgcatggg tagagctaaa accagatgca gatgcagttt atgatgaaga
     5401 gattactata aacttggatg aattaaaacc attagctgca aaacctcata gcccagacaa
     5461 tgtagatgaa gttgaaaata taggcaagat taaaatagac caagtggcaa taggaagttg
     5521 tacaaattct tcttatgaag acttaatgaa agtagcacaa atcctgaaag gaaataaagt
     5581 acataaggat gtgagtttgg taatagcacc aggttcaaga caagttatgg agatgattgc
     5641 tagaaatggg gctttggcag atataataag tgctggagct agaatactag aaaattcttg
     5701 tggtccatgt attggtatgg gtcaatctcc aggtactgac tctgtgtctt taagaacttt
     5761 caatagaaat ttctatggta gaagtgggac tttgtcagca caagtatatt tagtaagtcc
     5821 agaagtggca gcagtaagtg caatcaaggg agtgcttaca gacccaagag aatttgatat
     5881 taaatttaca aatttagatg taaatgagtt tttgatagat gatagcatga taattaaacc
     5941 tgctgatgta ggaagtgatg tagaagtagt tagaggacct aatataaaac ctttccctct
     6001 taatacagag ctaagtcagt ctataggtgg aaaagttata ttaaagacag aagataatat
     6061 aactactgac catataatgc catcaaatgc taagttactt ccttttagaa gtaatatacc
     6121 gtatcttgca aactattgtt tcaatactgt agatacagaa tttcctcaga gagcaaaaga
     6181 caataatggt ggatttatag ttggtggaga taattatggt caaggttcaa gtagagaaca
     6241 tgcagcttta gcaccattat atctaggtgt taaaggggta atagttaaga gctttgcaag
     6301 aatccacaag gctaacttga taaatagtgg tataattcca atggagtttt gtgatgagaa
     6361 agattatgaa aatatatctc ttttagataa tctggagata ccaaatattt tagacaatct
     6421 aggtagtgga attttagaag ttaagaatac aactaaagga acttctttta aggtcaaagt
     6481 agaattgtca gcaaaagaag ttgatgtatt aaaagctggt ggaaaattaa actacactaa
     6541 aaatcaagct aattaatatg aaaagaggga atattgaatg tataaagtga cacttatacc
     6601 aggagatgga ataggaccag aagttgcgaa agctatgaaa aaggtagtag aagctactgg
     6661 agtagaaata gaatgggaag aagttaatgc aggagaggca gttattgaag agtatggtac
     6721 tccacttcct gaatatatta tagatagtat aaaaaagaac aaaatagcta taaaaggccc
     6781 aattactact cctgtaggta aaggatttag gagtgtaaat gtaactttaa gacaggcatt
     6841 agatttgtat gtcaacttaa gacctattaa aagttttaag gggataaaat caagatatga
     6901 agatgttgat ttagtggttg taagagaaaa tacagaagat ttatatgctg gaatagaaca
     6961 taaaataggt gattatgcag ctgaaagtat aaagataata acaagaagtg caagtgaaag
     7021 aatcgttgat tttgcatgta actatgttaa agataataag agaaaaaaag ttacagctat
     7081 acataaggca aatataatga aaatgtctga tggattgttt ttagatgtgt ttagagaagt
     7141 tgcatcaaaa catggtgtag aatatgatga tttgattgta gatgcagcag ctatgaactt
     7201 agttttaaat ccagaaaatt atgatgtaat ggtaatgcca aatctttatg gagatatact
     7261 atcagattta ggtgctgggc ttgttggagg tttaggtata attccaagtg caaatattgg
     7321 taaagattgt gcgatatttg aagctgttca tggaagtgct ccacaaatag caggtcaaaa
     7381 taaggctaat ccaacagcac ttattcaatc atcagtaatg atgcttagat acttaggtga
     7441 atatgaaaat gcacaaaaaa tagaaacagc tttagaaaaa gtatttcttg aaggaagtaa
     7501 attgactgta gatttaggtg gctcagcttc tactacagag tttgcggatg aggtgtgtaa
     7561 gtatattgta tagattttat ttagggaaaa agtaaaatat agattgaaga aaagaggctt
     7621 tctcaaaata gagataagcc ttttttattg gaatgataaa atatatttga ttttatacaa
     7681 aagagagttc ctcataaacg aaaaagttaa ttttgggata tccattttaa ttttaaaatt
     7741 aattgattaa tacgcaaaga aagattaaat aaatttatta tttttaattg aactttcaac
     7801 caaaatatgg taaaatttaa aaagttgaac tttcaactaa aaatgaaaat attgattatg
     7861 tgaaattcta taaaacagaa actatttgac tttagccatg gtagtttcag tggaggtgtg
     7921 tttgattaag actttagata gtaatatatt aagagaagta ggtactttat ctagagcagt
     7981 aaattcaata aatgatatta agtacaagga attaaagtta caaaaaggac aatttacatt
     8041 tttgacaaga atctgtgaaa atcctggtat aaatcttgtg gaattatcaa atatgttaaa
     8101 ggtagacaag gctacaacaa ccaaggctat acaaaaatta ataaaggcag gatatgtaga
     8161 taaaaagcag gataagtttg ataaaagagg atataattta actccaactg ataaatcttt
     8221 ggaagtttat gagttgataa ttgaagaaga gaatagaagt atagagatat gttttgataa
     8281 ttttacagat gaagaaaaac aagtggtaac taagttatta gaaaaaatga gcaaaaacgt
     8341 agaaaatgaa tggtttaaag taaagagata gtaaataaat acattaactt aaggagatat
     8401 ttttatgatt agaaaagcga atatgaatga tttagaaagc attatgaaaa ttataaaatc
     8461 tacagtggaa gaaatgaaga cttataataa tacacaatgg gatgaaaact atccattaga
     8521 aaaagacttt gtttcagata ttaagaaaca agatttatat atttatgaag tggatggaga
     8581 agtagcaggg tttatatgtc ttaattatga agagccagaa gaatatttag gcttaaattg
     8641 gtcatctaat aaaaaagcaa tggtaataca tagaatggct gtaaatccaa actttagaaa
     8701 gacaggaata gcatctaagt tagttgattt tgctgaaaaa ttagctgtag aaaacaatgt
     8761 atcatactta aagagcgata catattcaat aaactcaaaa atgaatttat tacttacaaa
     8821 gtgtggtttt ataaaaatcg gagaaatgag ttttttagga aaggagaaat ctttctactg
     8881 ttatgataaa atattataaa gagcatttat aatacaaggg gtaaataata gattttcagc
     8941 attagtaagg gcataatata acctagtata tgctattatg aaagggatga tttatatgat
     9001 tggatttcta aagaaaagaa gaagtattag aaaatacaag gatgttgaag tagaaaaaga
     9061 aaaattagat aaaatattaa aagctgctct tttagctcca tcatcaaagg ggcttagaac
     9121 atgggaattt atagtggtag atgataaaga aaagctaatt aatttatctc aatgtagaac
     9181 taaaggtggt ggatttttct taaaaaatgc acctttagct attgtaatta ttgcagataa
     9241 agaaaaaaat gatgtttgga tagaggatgc aagtatagct gcttcataca tacaacttca
     9301 agctcatgaa ctaggtttag gttcttgttg gatacaagta agaaatagaa tgcatgatga
     9361 taatatagaa gctgataagt acattagaga agaattaaaa gtacctagca agtacagtgt
     9421 agaatgtata atttctatag gatatcctga tgaagaaaag aaagcatacg gtgactctga
     9481 tttagactat aaaaaggttc attttaacaa cttttagtat ataatatgtt gtattaagac
     9541 attatatttt tttctattgt taatatatac ttaaaattgt aaaaatatat ctgaatttta
     9601 ctaacttatc taggggttat gtacaaggat tttaaagata aatatagaaa ttaatgttta
     9661 taatcgatac ataacttgag taacaaaatt taaggaggtt aaagtatggg tatatttaaa
     9721 acaaaaatgg atgaggactg gaaagtaaac tacattaaag agtttaatga aatgagagat
     9781 tcttatgaga gtaaacttca aaaaaaacag tttgaggtag atagtttgaa atcagaattg
     9841 gatagactaa gaagctataa aaatagcctt aagccaaaag aaaaacaaat tactgatgat
     9901 gatataaata atataaagag tttaagaaga gatggcttaa gctataaaga aatttcaaat
     9961 caaacaagtt ggagcaaagc aactgtaagt agggtattaa atggacttta cgattagcat
    10021 atacatgggg tgatttaaat tgggatgaag tatagttaca aaaaatgcat aatagattct
    10081 attttattga tgtttatagt tcaaatatta aggatgattt tgaattatgt acttctaagt
    10141 caatttgaat ttactcttga aaattttaat attataaact taatttcttt tacattagtt
    10201 ggtttatcgt taattttatt tttaaaagat aattctttgt ataacaaggt tagaaataga
    10261 aaaatcacag aagcttttga agaaaataaa aacaatatac ttattgaaaa gtgtaagctg
    10321 atactttttg ttgtggtttt atctttagca ataattgtaa cttattgtac taagggatat
    10381 gtacttttta atgtaactat gatgactttg tctgtattga tagtcccaat atttgaagag
    10441 ttatttttta gagaatatat atggaactat ctaagtaatt ttataaagtc aaagagtaaa
    10501 attatctgta taacatctat actatctgga atttataata taggatacat agatgttata
    10561 agaaattatg taatattgta taacaattca agctacacct ttgaagttat tatttcaaaa
    10621 attatgatag gtactgtttt tggtattgta cttggattag ttaagtatag gtttagagat
    10681 gtagggtttt gtatattatt gaggagctta tttgctatat ttattaggta gatagtataa
    10741 tcggtttata aggttgtttt aaatttaatt ttaaatcaac cttattttat ttgtgtttta
    10801 taaagcactt atatattaat aactaataaa tttaataa
//
LOCUS       DAYASV010000125        10756 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYASV010000125 DAYASV010000000
VERSION     DAYASV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.128"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            78..1136
                     /locus_tag="ACGI68_003032"
     CDS             78..1136
                     /locus_tag="ACGI68_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HFH0139454.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTI
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRSIFSKNYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            1240..2424
                     /locus_tag="ACGI68_003033"
     CDS             1240..2424
                     /locus_tag="ACGI68_003033"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFH0139455.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMIVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            2631..3344
                     /gene="rpsB"
                     /locus_tag="ACGI68_003034"
     CDS             2631..3344
                     /gene="rpsB"
                     /locus_tag="ACGI68_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFH0139456.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            3423..4334
                     /gene="tsf"
                     /locus_tag="ACGI68_003035"
     CDS             3423..4334
                     /gene="tsf"
                     /locus_tag="ACGI68_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFH0139457.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            4419..5123
                     /gene="pyrH"
                     /locus_tag="ACGI68_003036"
     CDS             4419..5123
                     /gene="pyrH"
                     /locus_tag="ACGI68_003036"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFH0139458.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            5149..5706
                     /gene="frr"
                     /locus_tag="ACGI68_003037"
     CDS             5149..5706
                     /gene="frr"
                     /locus_tag="ACGI68_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFH0139459.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDALLSKKEKDIMEV"
     gene            5709..5912
                     /locus_tag="ACGI68_003038"
     CDS             5709..5912
                     /locus_tag="ACGI68_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139460.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            5941..6675
                     /locus_tag="ACGI68_003039"
     CDS             5941..6675
                     /locus_tag="ACGI68_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFH0139461.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            6688..7467
                     /locus_tag="ACGI68_003040"
     CDS             6688..7467
                     /locus_tag="ACGI68_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFH0139462.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
     gene            8291..10102
                     /locus_tag="ACGI68_003041"
     CDS             8291..10102
                     /locus_tag="ACGI68_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0139463.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVATVLSKTFNIKETCARICSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSMMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVKKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(10190..>10756)
                     /locus_tag="ACGI68_003042"
     CDS             complement(10190..>10756)
                     /locus_tag="ACGI68_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139464.1"
                     /translation="KINQFERSYDIGIFKNEQSIYKFIEKIPFVKKDSSNFNYTNYYM
                     EFEAIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFINGETL
                     VDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTNDSILY
                     LLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         4343 a         1087 c         1881 g         3445 t
ORIGIN      
        1 ttaagtatat aaaataaaat aatatccaaa ctaatacaaa atagtaataa ttgttttgta
       61 ctggaggata tatatttatg aattgtaaat ggatatctaa aatagatgag agaaaaaaat
      121 gtcatagaga agcagattct tctggatatt gtatctttca taaagaaaat aagtctgatg
      181 aagaaataca gttgatgatg gatactattc ataaagaaga gataagtgaa tttaatggat
      241 ttgtttttga aaatgaattt aatgcggaag aaatattaac ttacaattat aaaatactgg
      301 atttttctga atccatattt aaacaaaaag caaattttaa aaaatatatt tttaaaaaga
      361 atattatttt taactataca gaatttagag ataaagtttt atttaatggt tgtgtattct
      421 tagaaaactg tgattttaat agaagcatat ttagtaaaaa ctatataaat gaccgtatat
      481 ttgaaaaagt gaaatttaaa ggacctgatt tggtagttaa taaagtagaa aattttccaa
      541 gaatggatgg aataattttt agcatgtgta caaaatttgt gttaaagaat gttgaatatg
      601 gaaaaagtga gtatgagcat ggcaagataa attatagaat agctagaaat caagcaacaa
      661 aaattggaga atatgagatg ataggatttt actattataa ggaaagaatt tatagtagta
      721 aaataatgaa acgttctaat tatcctactt tcagtgatta tttagttgaa aaattttttg
      781 accaaatagc acgttacaca acaggttatg gagaaaagcc atggaatata ttattagtta
      841 ttatagcaat aataagtgtt tttgctttat tatacttatt tgtaggcata gaaagttcaa
      901 atagcacact tgtagcttta gatataaata atataggtga ttactcacta agtgagattt
      961 tcaaaatgta tatggattta tggtatttta gcatggctac atttagtaca gtaggatatg
     1021 gagatatggt tgctacaagt ttgattggga aagcattggc aggtatagaa gtattttttg
     1081 gagttacaat aggagcaatt tgggcatctg tgattattaa aagaatgata aggtaatatt
     1141 tgtgtaaata tggataggta ttaattacta gatataaatt ggactaaata gggaaaattt
     1201 aaatataaat atacttaata atccaagaag gtgaaaaatt tgaaaaatgc actaaaaaaa
     1261 attatatcat tagccatgat tctagttttt ataataccaa attattcata tgcagatgat
     1321 aaatctgtag acaagtattc aaaaacatct atacttatag accaagaaac aggtagagtt
     1381 ttatatagta aagaacctga tatgaaggta ccattagcta gtttaagtaa gatgatgact
     1441 tttttacttg caatagaagc tattgaaaat aaagaagtaa aagaaactga taaagtaaaa
     1501 atagacaaat ctattgcatc agtaaaaggg tcaagttata aacttaaaga tggtgaagaa
     1561 gtgccactta tagagcttat gagaggtctt atgattgtat caggaaatga tgcggcaatt
     1621 gctatagcaa aacatatttg taaaacgaaa gaagcatttg tagatagaat gaataaaaaa
     1681 gcaaaagaaa taggaatgag taatacacat tttctaaatc caaatggtct tccaatatac
     1741 gatttaagca accccaaaaa accagcaaaa gaaaatatct ctactgcaaa agatatagct
     1801 attcttggaa aatacatgtt tgaccattat gaaaaacaag taacagctat aactgatatg
     1861 gagacatata caaattcaga tagaagtttt gaaaaaagca atacaaatgc attattaaga
     1921 attataccag aagtagatgg tataaaaact ggatatactg gcaatgctgg atattgctta
     1981 tcattttcta tgattgtaaa taaaaatgat aaaaatgaga aaaaacgtag agtaataggg
     2041 gttgtattag gtactaacca taaaaataaa agaacctctg cttcattagc tctactgaaa
     2101 tatggaaaag aaaatttcaa tatgaaaaaa gtaattgata aagatagctt tattggtaag
     2161 aaatatataa aaggaatgaa agagttagag gttacattaa aagcaaaaga tgaactttat
     2221 acaatattaa aagatgatga aagtataaaa tcagaagtta agattaaaaa tatagagtac
     2281 cctgttaaaa aaggtgatac tatgggtatt ataaaatatt atactaattc aggtgactta
     2341 cttggaagtg tagatatagt aagtaataat agtatagata acgtatcatt aaaaacaaaa
     2401 ttaaaaatga aatttgcaaa ttaaattcaa aaaatggtat aaaatacaca aaaaaatata
     2461 aaaatagaaa atatggaaaa aatattgtta tttcttcacc tctgtgatat actactagag
     2521 gtgaaaaaat aaaataaatc acacacagca ttgggatcct taaaaggtgc ctattacatg
     2581 gttttttagg gagaagatca agctggaggt ataaaaccag gaggtaagat atgtcagtaa
     2641 tatcaatgaa acaattatta gaagcaggtg ttcactttgg tcaccaaaca agaagatgga
     2701 accctaaaat ggctaagtat atattcacag agagaaatgg aatatatata atcgacttac
     2761 aaaaaacagt taaaaaagtt gaagaagctt acaagttcac taaagaagta gctgaaacag
     2821 gaaagccaat cttattcgta ggaactaaaa aacaagctca agatgcgata aaagatgaag
     2881 ctgaaagatg tggaatgtac tttgttaatg aaagatggtt aggtggaatg ttaacaaacc
     2941 acaaaactat aaaaacaaga ataaataaat taagagaatt agaaaaaatg gaagaagaag
     3001 gcgtatttaa tgttcttcct aaaaaagaag ttataaaatt aagagctgaa aaagaaaaac
     3061 tagaaaaata cttaggtgga ataaaagaca tgcctgaatt accaggtgca atgttcgtag
     3121 ttgacccaag aaaagaaaac atagctattc aagaagctca tagattaggt ataccagtag
     3181 ttggtatagt tgatactaac tgtgacccag aacaattaga ctttgctata ccaggaaatg
     3241 atgacgctat aagagccgta aaattaataa ctggtgctat ggcaacagct gtaattgaag
     3301 gtagacaagg tgctgaggaa gaagtagctg aagaccaaga ataataattt gacaattaaa
     3361 tggtaagggt cgcccttacc atttataata tggaatagaa taagtaggag gaaacttaaa
     3421 aaatggctaa tataactgct caaatggtaa aagagttaag agaaagtaca ggcgcaggaa
     3481 tgatggactg caagaaagct ttacaagaag ctgaaggaaa catggaaaaa gcagtagatt
     3541 tattaagaga aaaaggatta tcaaaagctg ctaaaaaagc tggtagagtt gctgctgaag
     3601 gtctagttgc aatagaaatg aatgatgata atacagttgc ttctatggta gaagttaact
     3661 cagagacaga tttcgttgct aaaaacgaag acttcaaagt gttcgttaaa gatgctgctt
     3721 gtatggcatt agctactgat aaagaagata tagcttcttt attaggtgaa actcataaag
     3781 agggaataac tttacaagaa gttttaaata atagagttgc aaaaataggt gagaaattag
     3841 actttagaag atttgctaaa gttgtaacta atggacaagt tgctggatac attcacggtg
     3901 gtggaaaaat aggtgttctt gttgaaatgg aaacagaagc tagagatgct aaagttttag
     3961 aattaggtaa agatgtagct atgcaagttg ctgctatgaa tcctaagtat gtttcaagag
     4021 atgaagtaga tgctgaatac atagcacatg aaacagaagt attaactcaa caagctttaa
     4081 atgaaggaaa accagctaat atagttgaaa aaatggttaa aggaagatta gaaaaagaat
     4141 taaaagaggt ttgtttatta gagcaaactt tcgttaaaaa tccagatata actgttaaac
     4201 aattagttgc tgatgttgct aaggcagtag gttctgatat aaaagttgtt aaagttgtga
     4261 gattcgaagt tggtgaaggt atacaaaaga gagaagaaaa ctttgcagaa gaagttgcta
     4321 agcaacttaa gtaataacca aagagagaac acgcaggtgt tctctttttt aaaaaataga
     4381 aaaaattgga tatgaaggat tgaaatagga ggtttctgat ggataaacca atgtacaaaa
     4441 gggtattgtt aaaattaagt ggagaagcat tagcaggaga aagaggtttt ggtataaata
     4501 atgatgtagt taatgatatt gctattgcta taaaaaaaat acaagaaata ggtgttgaag
     4561 tagctgtagt agtaggtgga ggaaacttct ggagaggcag aaccagtgaa ggaatggaca
     4621 gaactactgc tgactatata ggaatgttag ctacagttat gaatgcaatg gcgctacagg
     4681 atgcactaga aaatatagat gttgctacaa gagttcaaac tgctatagac atgagacaaa
     4741 ttgcagaacc atatataaga agaagagcag ttagacactt agaaaaggaa agagttgtta
     4801 tatttggtgc aggaacagga aacccttact ttacaacaga tacaactgct gctttacgtg
     4861 ctgctgagat ggaagcagaa gttatattgt tagctaaaaa tgttgatgca gtatatgaca
     4921 aggatccaaa agtacatgca gatgctaaga aatttacaga attaagttac atggaagtta
     4981 tacagaaaga attaaaagta atggattcta cagctacatc tttatgtatg gataataaaa
     5041 ttccaataaa ggtatttgag cttacaacag agaatataat aagagcagta aaaggcgaaa
     5101 atataggaac tactgtaaaa taatgaagaa tgaaggagga gtaaaattat gaaactggaa
     5161 atacataagc aattagaaga gaaaatgaat gggacaattg atgctttaaa gtttgaattt
     5221 ggaactataa gagcaggaag agctaatgca caaatgctag acaaaataag agttgattat
     5281 tatggaactc caactccaat aaatcaaata ggtgctatat ctgtaccaga accaagaata
     5341 ttaatgatat ctccttggga taaatctgct atgcatgaaa tagaaaaagc gatagctaac
     5401 tcagacttag gattaaatcc atcaaatgat ggtgaggtta taagactttc tgtacctgct
     5461 ttaacagaag aaagaagaaa agaattagct aaaaaagcta gtaaagctgc agaagaattt
     5521 aaagttagaa taagaaatga aagaagagac gccaatgaga aaattaaaaa gatggaaaaa
     5581 ggcggagaat taactgaaga tgaattaaaa aaagcacaag atgaagttca aaaaatgact
     5641 gataaattta taaaagaaat agatgcatta ttatctaaaa aagaaaagga cataatggag
     5701 gtataatatt gaaaaaatat tataaccttt taggtcttca tgtagatgag gtaaaacaat
     5761 attttgatga gcaaaatata aattataccg taaaatctat tgaaggtaaa aaggataaag
     5821 aaaaacttgt agttcctaga gttataaaaa tatctgagat aggtgatagt gtagaactga
     5881 taatcacata cttttctgac tccttagttt aaggagtcag aacaatattg gaggcaaaat
     5941 atgaataaca acattatata tgatatagac ttaaataaca tacctactca tattgctatt
     6001 ataatggatg gaaatggaag atgggcaaaa gctagatttc ttccaagaac tgcaggacat
     6061 aaggctggag tagaaactat aagagatata gtaaaagaat gctcaaaatt aggtgttaag
     6121 cacttaacat tatatgcatt ttcaacagaa aactggaaaa gaccgaaact tgaagttgac
     6181 acacttatga acttattatc tacatactta agaaatgaaa tagctgaact tcaccaaaac
     6241 aatgtaaaag taactgctat aggagatata agtgctttac caaaaacttg tataagagaa
     6301 ttaaactcag ctaaggaact gacaaaaaac aatacaggag ttaacttaaa tttagcatta
     6361 aactacggaa gtagagcaga tataaaaaat gctcttatag atatagtaaa aaattgtgaa
     6421 agtggtaaaa tagatataaa caatattgat gaagatatta taaaaaacta tttaagcaca
     6481 aagtctatac cagatccaga cttggtaata agaacaagtg gtgaacaaag attgagtaac
     6541 tttttacttt gggaagttgc ttattctgag ttttacttta ctgatataca ttggcctgat
     6601 tttaaaaaag aagaactaca gaaggctata tacgtatatc aaagaaggga tagaagattt
     6661 ggaggactta agtaaggaga ttattttatg cttacaagaa ttattgcttc actagctctg
     6721 gttccattat ttctatttgt tgtatatgga ggtattcctc tatatatagc tgagacagca
     6781 atagtttata ttgcattgca tgagttttat aaggcattta aaataaaaga tgtacatcca
     6841 atattcatta taggatattt attttctatt tatttagctg taaaaaatat ttttaattta
     6901 ccattagaat atacttatgc agtaattttt attttatttt tagcaagtat aatttatatg
     6961 ttgatgggca aaaacaatgt tatagatgtt tcgattacgt ttttaggagt tttttatata
     7021 ggagtatttt tagattttat aattataact ataaatggat ttgaaaaagg tagtatatat
     7081 gtatggctta tatttgtgat atcttttatg acagatatat ttgcatattt tagtggttat
     7141 ttacttggaa aacataagct aattcctaaa gttagtccta aaaagactat agaaggagct
     7201 attggaggaa taataggaag tacactttgt tgtattttat ttggatattt atttggcatt
     7261 gatttgcttc aattagcgat aataggaagt ataggaagtg taatagctca attaggagat
     7321 ttatttgcat cttctataaa gagatatgta gggataaagg attatggaaa gataatacct
     7381 ggtcatgggg gtatattaga tagatttgat agtgttatat tagttgcacc ttttgtatat
     7441 agtgcgatta aattttttat tagataaaga ttggttggat ttaaaagagc aatctataat
     7501 tgctagtaat gaaaatagct atgtaacttt taaatgcaga gatattctgc agttattagt
     7561 tacatggcta ttttcattgt tcaaaaattt agaagacctt ggggaattgt ataagaatag
     7621 gtattacttt ataatagtat actatatttg tagtgagtat attttttata ctgtaactat
     7681 cattaaaaaa tatttttttg tttaataatt tcgatatttt tagctttatt atatatttat
     7741 aatatatatg tataaatgta actaatttat tatccattta taaattttat ttttaaatat
     7801 aattaataag agataagtaa attattggtc ttattgcaca aaaatagata taatttaagt
     7861 aatataccaa aaaaaaggaa tatatttaca taatatgata aaataaattc aaagagttaa
     7921 attgtaggta aatagtattt taattattta ttaaaatata gattatgtat atgccaaatt
     7981 taaaaagaga cataaagaca ttatacaaat atattattgg ggtgatattt tgtaagaaaa
     8041 gaagtgatgc agtaaattaa ttgagtaatt taaaaatgat ttaatgataa tgttgatgaa
     8101 tgtttaaaaa gaaattttag aaaaacgtat attgctgttt gtgttggagg tgatgaattt
     8161 gtagaattta tagggtataa atgattaaac tatttaagat aaatacatat cactgtaaaa
     8221 tattgataat cattttttat aagagattga ttatttgcaa attatatata ttacaaatga
     8281 ggtaacaata atgaagaaaa aatatgattt tttaatttat tctgctattt ggctagttat
     8341 tattattctt tttatatttt ctctatatca tagtatagag catattaaaa ttataagtca
     8401 cactggtact attcgcagtg aaactcaaaa ggttgttaag caagaattga ataatgaaag
     8461 aaatgatgac ttgataaaac gactggataa tattctgata aagctaagaa caggtaatgg
     8521 agaaaatgga tttcaaagat gtgataataa agaatttcaa caaaaactaa atcaaatgga
     8581 ttctatgtgg gaatctatga agaaagaaat tataaaagtt agaaatggtg cttcaggtga
     8641 taaattatat aaattgagtg aagagtattc tgtgttgtct aatcaaattg tttttatatc
     8701 tgaaaaacac tctaatgcaa aattgtattc ttttgctact gcactattta tttatcttat
     8761 attctcaaca ataagtcttt taatttggga atattataat aaaaaaaggt ttaaaagaat
     8821 attttatact gataatttaa caaaaataaa aaatcaggtt gcatttgaaa acagagcgat
     8881 tgaaattttg cacaatgcat ctaacaaaga gtatgtactg ctaaatattg atattgacaa
     8941 ttttaaatat attaatgata cacatggata tgaatatgga gataaagttc taataatagt
     9001 tgcaactgtt ctttctaaaa cttttaatat aaaagaaact tgtgcaagaa tttgttcaga
     9061 taattttgta atacttgcaa aatatagaga ttctctttta gaggatattc gggaaatgct
     9121 aactaatgct ataatctctg aattagatat gaatgtaact caaactatat cttattgtat
     9181 aggagcttac ttagttgaaa ttgataattt aggttataaa tctatcaatt ctatgatgga
     9241 taaggcaaat attgcacaca aggtaagtaa aacaagagga atttcctcca cagtatggta
     9301 taatgaaaat ctgttaaaac agttacaaat ggaaaatagt atttataatt atatgtataa
     9361 agcgatagaa aatgaagaat ttcatatgta tttgcaacct aaatttcaaa tatcttcttt
     9421 aaatgttgtg agcgcagaag ctcttgtaag atggttttct ccagaacttg gttttttatc
     9481 accagatgaa tttattccat tgtttgaaaa aagtggattt attattgaat tggactttta
     9541 tatgttaaaa aaagcgtgtt catttgtaaa aaaaacgttt atgaaaaaaa atcaatatac
     9601 atatccgatt gcagttaatt tttcaagggt tactatttac caaaatagtt tttatcaaag
     9661 gtttttagat actgttaaag aatatgaaat tccttttaag tatatagaga tagaggtaac
     9721 tgaaagtgct tttaatgaaa tatctcaacc agttatttca attttagagg aacttaaaaa
     9781 gttaggattt ttaatttcta tggatgattt tggatcagga tactcttcat taagtctttt
     9841 atgttctcta tctattaatg gattgaagtt agataaaagc ttacttaaag aaacatttaa
     9901 ccgggagaaa gtatatagta ttattcagtg tattattgaa atgtcacatc gtataggcat
     9961 gtctgttgta tgtgaaggta ttgaaactaa aaaagacttg gaatttttaa atacagttaa
    10021 atgtgatgta ggacaagggt tttatttttc taaaccgata gaagaaaaag aattttttaa
    10081 caaatatgta actaaaaaat aatataatta tttttatcag tctatacatt gagtcatttt
    10141 atatctcgtt aaatgactca atgcactgtt atgtatttta aaatataaat tatttaaaac
    10201 gttttttata cttatataga aattcttcta tattttttga ttgttctctt atttcaactg
    10261 cttctatatc tagtaaataa agtatagaat catttgtaag ttcaacatat tcaccatctt
    10321 ctgaaccgag tgcatgtctc tcaattttta atcctttttc ttcataatat ttttttgttt
    10381 ctatatataa tgattctcgc ttttgtatgt agtctttcac ttcattattt ggaaagttat
    10441 atgaatccac taatgtttcg ccatttataa aagttttctc ggtagcaact ttttcatccc
    10501 aacaatggat ttcttcccaa gtaaaaaata tctcagttcc atctggaatg aatgaaaacc
    10561 tggtaataat atataaataa ttattgaaat ttacttcata atattcagga atagcttcaa
    10621 attccatata gtaatttgta taattaaagt tactactatc tttttttaca aatggtattt
    10681 tctcaataaa tttatatatt gattgttcat ttttaaaaat tcctatatca tagcttcttt
    10741 caaattgatt aatttt
//
LOCUS       DAYASV010000126        10710 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYASV010000126 DAYASV010000000
VERSION     DAYASV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.129"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..410
                     /locus_tag="ACGI68_003043"
     CDS             <1..410
                     /locus_tag="ACGI68_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFH0139465.1"
                     /translation="IANMFMKKDINKEFDIDFILSKEVICNYTKEIFQNLYKKEQSIG
                     KKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKIYLFRKFGVPFVVG
                     CLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI68_003044"
     CDS             complement(710..1297)
                     /gene="rbr"
                     /locus_tag="ACGI68_003044"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0139466.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(1383..1820)
                     /locus_tag="ACGI68_003045"
     CDS             complement(1383..1820)
                     /locus_tag="ACGI68_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0139467.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            1924..2922
                     /locus_tag="ACGI68_003046"
     CDS             1924..2922
                     /locus_tag="ACGI68_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HFH0139468.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(3042..3968)
                     /locus_tag="ACGI68_003047"
     CDS             complement(3042..3968)
                     /locus_tag="ACGI68_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFH0139469.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            4353..5957
                     /locus_tag="ACGI68_003048"
     CDS             4353..5957
                     /locus_tag="ACGI68_003048"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HFH0139470.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENHIRPVMIHAQTVREDQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            6773..8044
                     /gene="serS"
                     /locus_tag="ACGI68_003049"
     CDS             6773..8044
                     /gene="serS"
                     /locus_tag="ACGI68_003049"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFH0139471.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
     gene            8322..9827
                     /locus_tag="ACGI68_003050"
     CDS             8322..9827
                     /locus_tag="ACGI68_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0139472.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            9941..10639
                     /locus_tag="ACGI68_003051"
     CDS             9941..10639
                     /locus_tag="ACGI68_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0139473.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
BASE COUNT         4199 a         1210 c         1676 g         3625 t
ORIGIN      
        1 atattgcaaa tatgtttatg aaaaaggata ttaataaaga atttgacatt gattttattc
       61 tcagtaaaga agttatatgt aattatacca aagaaatatt tcaaaattta tataaaaaag
      121 aacaaagcat aggtaaaaaa gaactgctta tattgagcat aatagagaag tttttagtta
      181 aatctataat aacttttgaa gagttaaaat tgatagtgaa tataatagaa gaaaatttta
      241 catcaatata caatgaaagt ggaaagattt atttgtttag aaagtttgga gttccattcg
      301 tagtaggatg tctggattat aaaaaagtaa agtttgaaaa agatgtagtt aagattattg
      361 aggttgactt gacaaaagaa ataaatgata taagtataaa gttatcatag tgtattatct
      421 tatataatgt attataataa tctacttaaa atacacttta tttaaatatt tagtttaaag
      481 atgagatttt atgtgagtgt gtaaaagtta aagatagatt taagtaggtt atagggatta
      541 gtaaacaata aaatgaacca tgatgaacta ctataaattg tatttaaact acattttcta
      601 cttatagtaa tatcaatggt tcattttatt gttattaaga taatacagta tttataaaaa
      661 tacttgatat aaatatagta aattgtttaa atagaattat tgccttaatt tattcaaata
      721 atgagttttt aaaatctaag aaatatcctt gtggatgaag acatacagga caagctttag
      781 gagcttcttt acctatatgt atatggccac aatgtgtaca tatccattgt atatcttcat
      841 ttcttctgaa taaagtacct tcttctaaat cagtagcata tctaccaaat ctatcaccat
      901 gaactttttc tatttcagct atattttcaa ataacttagc tatagattca aatccttctt
      961 cacgtgctgt ttttccaaag tttttatata catcatccca ctcttgaagc tcattatgtt
     1021 gtgcagcttt aaggtgttgt gctgtcttgt catataaatc tacagggtat gctcctgata
     1081 tacttatatt ttcaccagca aattctttta atttagaaaa gaattgtttt gcatgtgatt
     1141 tttcttggtc agcagtgtaa ttaaataaag actctattat atataatcct tctttttttg
     1201 ctacagatgc agaaatattg tatctatttc tagcttgact ctcacctgca aaagctctta
     1261 ataaattttc tttagtttta ctatctttta aattcatatt ctacctctct atcacaataa
     1321 atttttacaa tatttgattc tttttattta ttattccaat aggagatata attatgctca
     1381 atttagtcag catttttaaa attatcatta atttttttta aaattctaag aaattcttgt
     1441 ttttcatttt ctgtaatatt atggtatgcc gtctctttaa cttgtgttga gatgctatta
     1501 aaagtatctt ctataagcag tgcctttttt gtaagcttta tatacgtgat acgtttgtca
     1561 tttgtacatt tttgtctttc taaatatcct aggtttataa gtttattaac caacactgta
     1621 acagtggatt tatcttttcc tattttagct gcaatttctt tcattgtaag tattccatta
     1681 ttttcataaa gtgcagttaa aatatttcca tgagtaggta ttaactcacc taaaccattt
     1741 ttactgagtt tattttcaat gaatttaatc atactagcct ttgttttact tatgaaatgt
     1801 acaatatatt tatcttccat aaattattct ccaatcttta atctatttaa attagtttga
     1861 cataaaacta tttataaact ataattagtt taacataaaa ctaaatgtat tgggaggaaa
     1921 tttatgaata aaaaatttaa atcactatat attacagaag aagaaggaaa ttttagaaga
     1981 aatatagttg aaagagaagt ggatacattg ccagaaggcg aagttctaat aaaagtaaaa
     2041 tattcttctc taaattacaa ggatgcactt tctgcaaatg gtaataaggg tgttacgaga
     2101 aagtatcctc atacaccagg aattgatgca tctggtatag tagaatctag tagttgtgat
     2161 gattttaaaa ttggagaaga agttttagta acaggttatg acttgggaat gaatacttct
     2221 ggtggttttt cagaatacat aagagttcct tctagttggg tagttaaact tcctaaaaat
     2281 ctttcactaa aagaagcaat ggattatggt acagcagggt ttacagcagc tttatctgtg
     2341 tataaattta tagattctgt agacaagaat atgggagatg tattagtcac tggtggtact
     2401 ggaggagttg ctgtaattgc aactaaaata ttgattaaat taggttattc agttgtagtt
     2461 tcaacaggaa aattagaaga acaaaaagaa gtattactaa acttaggtgt taaagatgtt
     2521 atacataggt cagaagtgga tgataactca ggaagacctc tgcttagacc aagatgggct
     2581 ggagtaatag atacagtagg aggaaatact ctagcaacag caataaagac aactaattat
     2641 tgtggagcag taacaacttg tggtaatgca ggaggagttg attttacaag ttcagtatat
     2701 ccatttatct taaagggaat tactttatat ggtatagatt ctgtggattg cccaatgaac
     2761 ttaagattaa aaatatggga tttattatca aatgaatgga aattagatga tttagatgat
     2821 atggtaaatg tagtatcatt ggaagagtta aatgatagta tagattcaat aatggcagga
     2881 aaacatgttg gaagaacagt agttgattta gaaaaaatat aggtgaataa atttataatt
     2941 aggattatag gttttaagca aaaatgagat acttgaaatt tacttgtgag tatctcattt
     3001 ttttatgtaa aaagatttat gtagatgttt gtgtaaaaag attattcatg aacaattaat
     3061 tcaaatacat ctggtcttga atagtgacct tctgaatcaa aatctagtct actaagtact
     3121 attttttcta ggtctaaatc agctattagc atttcttctt tattgaaaat aggacctgct
     3181 acatattttc caaaagggtc aactatacaa cttcctcctg gacacataat ttctggttct
     3241 gcatctagct ctttatagta gttaaggtca gttggataca tatttttttc aacatattga
     3301 ttgcatccaa ttacaaaaca tcttccttct aaagcaatat gcttcatagt tgcttgccac
     3361 tcttctcttg aatctgcagt aggggctatg taaagtttta cacccttagc ataaagagct
     3421 gttctagcta atggcatata attttcccaa caaatcaacg aacccatttt tccatatgga
     3481 gtatcaacta ctgtaagagt acttccatct ccttctcccc aaatacatct ttctgttcct
     3541 gtaggtttaa gttttctgtg tttaccaatt agcttacctt ctggagagaa aaataagttt
     3601 gtgcaataaa gggtacaatt tatatcatta ccatctcttt ctgttatacc cattgataga
     3661 tatactcctg cttcttgtgc tgcttttcct aataaatctg ttgtagcact aggaactggt
     3721 acagaattat catagtatct tttccaatct tctctacctt ccattgttct acttccaaca
     3781 acaaatccaa atgaaagacc tcttggatat gcaggtatga atgcttcagg gaaaactacg
     3841 atatttgccc cttttctacc agcttcttta attaatccta atgctttttc aacagttttt
     3901 tctaaatcca ttatgactgg tgacgcctgg acaacagcaa ttcttacatt tttattttcc
     3961 ttgctcataa aaacctccta aaaaatattt aaattttttg cattgaatag attacatgag
     4021 atgttatttg agaactaaga tacttatatt gtgaataata atgtaactgt atatttaaaa
     4081 taatacatca tgatttaaaa aattacaatt gtacagatat aattgagttt agatatttaa
     4141 atttaacaac ttataatatt ttttcaatta gtataaagcc tattaatttg tcttatttac
     4201 aatgaatagt atttacttct tggcaaacaa taattaaaaa taaaactttt atatgattaa
     4261 actctaaaga aaaagtttat aaagaagata atataattaa gcagaaaata atgaaaaaaa
     4321 tttattaaag tagacaacgt aaggagaatt ttatgaaaga aaaaatttat tacaatggaa
     4381 atatcatcac aatggaagat tcaatatgtg gagatgctat tcttataaaa gacaaaataa
     4441 taaaaaagat aggaacaaaa gaagaagtat ttgctttgaa aaataaagat acagaaatta
     4501 tagatttaca aggtaagaca ttgatgccat cttttataga ttcacatagt cacttgatag
     4561 cttttgctac tactctaaag ctagttcctt tagaagatgc aaccagtttt aaagacatag
     4621 taaaaaaaat acaggatttc aaagagtcga ataatataaa aaaaggagac tggataattg
     4681 gattttcata tgataataat tttttagaag aaaataagca tccagataag agtgtactcg
     4741 atagtgcatc ttcagaaaat ccaatactaa tttcacatgc ttctggtcat atgggagttg
     4801 caaatacatt ggggttaaaa cagcttggtg ttaccaatga aacacgtgac ccagaaggtg
     4861 gacatatagg tagagtagaa ggcagtgaag aaccaaatgg atatttagaa gaaaatgcat
     4921 tttttaatgt agcatctaaa ataaaacaac catcatcaaa tgagattttt aattcaattg
     4981 aaaaagcaca gaacatatat ctaagctatg gtattacaac agctcaagaa ggtttaatgg
     5041 aagaaaatca atttaacatt ttaaaagcaa tggcaaatca aaataaatta aaaatggatg
     5101 ttgtaggata tgtaaatttg aaaaaaagta agtctgtagc agataataat agggagttta
     5161 taaaaaaata tataaataga tttaaaatag gaggttataa gattttctta gatggttctc
     5221 cacaaggtaa gacagcatgg ttgagtagac catatgaaaa ttcagatgat ggttattgtg
     5281 gatatcctat atacaaagat gaagaagtag aaaagtttat agatatttca ctaaaagaaa
     5341 agatgcaact cttaactcat tgtaatggag atgcagcagc agaccagttg atagatgcgt
     5401 ttgagaaagt tttaaattta aaagagcaaa gtagtgaaaa tcatataaga ccagttatga
     5461 tacatgctca aacagtaaga gaagaccaga ttgatgatat gaaagtaata aatatgatac
     5521 cttcatactt tgtagctcac acatattatt ggggtgatat tcatatcaaa aaccttggag
     5581 aagatagagc gtttaagatt agcccactta aaacaactat agaaaaggga ttaatttaca
     5641 ctcttcatca ggatacacca gtaatagcac ctaatatgct ggaaacagta tggtgtgctg
     5701 tcaatagaat tacaaaaaaa ggtatacaaa taggagaaaa tgaaaagata agcccattag
     5761 atgctttaaa gggtgttact ataaatgcag catatcaata ttttgaagaa gataaaaaag
     5821 gttcaattaa agaagggaaa ttagctaatt tgataatctt agatgaaaac ccacttacaa
     5881 tcgaccctat gaaaataaaa gatattaaag tattgcagac tataagagaa ggagaagtat
     5941 tgtattcttt gaagtagatg aaagttagat attttataat ctataaaaaa tagttttata
     6001 taataagtta ggtagaatta aataagatta cagttaaatc aacaattata tttttattaa
     6061 attttaatta ttgtattgaa gatttataag tataatttta ttgattctac ctttttaaat
     6121 aactgtcgat ttattaaagt tatctatgta ctatattaaa caattaagta gttattaatt
     6181 gaacttatta gtttattaaa aaactatgta tattactttg cagtaagact ttggaactaa
     6241 atctataata gcaaatttaa atttattaaa tagattacct tataacattt catataaaat
     6301 atattgtgaa tctgagaaaa ttactatttt tctttgagta ttttttgtaa gcagttctaa
     6361 aagattcaat ttcataccta ttacttgaat cattcattaa aataattcta atttaaaaaa
     6421 taattctatc caaataatag atatatagga ctatgttata atataaaaaa atttatataa
     6481 aagttaataa ctatacaaga ttgataaaga aaaagtcaaa atagataatt gattagcaaa
     6541 atagatataa ttataatgaa gttaaatcta gcaatattct cctaaaatta aattacattt
     6601 atttaattaa atataatttg acaatggaca aaaaagtgag taaaatataa ataaaataaa
     6661 aataacaggt tcggataaag aaaaaggttt cttaacctca agcataaagt atattatcta
     6721 agttatttga gattaagaga taaatccaat cttaggtact gaggaggaaa aaatgcttga
     6781 tataaaaaga ataagagaaa atttagattg tataaaaaaa gcaatggaaa gaaggggcga
     6841 aaaagacttt aaccttgatg aagtagttaa gttagatgat gaaagaagaa agattcttca
     6901 agaagttgaa gttatgaaaa atgaattaaa tacagcatct aaaaatatac caaatcttat
     6961 aaaagaaggt aaagatgtag aaaatgaaaa gattaaacta aaagagttat ctgataaaat
     7021 taaggtaata gaccaaaatc ttaaagaagt agaagataag atggaatacc tattaatgag
     7081 aataccaaat gtaccacatc cagaagtacc acaaggtgaa actgatgaag ataatgttga
     7141 agtaagaact tggggaaaga ctactacctt tgattttgaa tctaaggctc attgggaaat
     7201 tggaacagaa ttaggtatac ttgattttga aacagctagt aaaataactg gttcaagatt
     7261 tactctttac aaaggattag gtgctagatt agagagagca ttattaaatt tttacttaga
     7321 cacgaataca aaagtaaatg gatatacaga agttattcca ccatttatgg caaatagaaa
     7381 tagtttttta ggtacaggac agttacctaa gtttgaagaa gatatgttta aaatagaagg
     7441 tatggactat tttatgattc caacatcaga agttccttta actaatatac atgctaatga
     7501 aatacttaaa tttgaacaat taccgataaa ttatacagct tatactccat gttttaggtc
     7561 agaagctggt tctgctggaa gagatacaag aggtttagta agacaacatc agtttaacaa
     7621 agtagaaatg gttaaaatag ttgcaccaga agaatcttac aatgagttag aaaagcttac
     7681 taacaatgcg gaaactatgc tacaattatt gaatctgcct tatagggtag taaagatatg
     7741 tacaggtgat ttaggattta cggcttcttt taaatatgat gtagaagttt ggatgccaag
     7801 ttacaatagg tatgttgaga tttcatcttg ttcaaactgc gaagatttcc aagctagaag
     7861 agcaggaata agatttaaaa gagataaaga ttctaaagct gaatatgtac acacattaaa
     7921 tggctctggg ttagctattg gaagaagtgt tgcagctata ttagaaaatt atcaacaaga
     7981 agatggttct gtggttgttc ctgaagtttt aagaccatat atgggagttt ctgttattaa
     8041 gtaagagtta aattatgtat ttttaagcaa tacaataata tagaggtaat aaaaaataat
     8101 taatataaaa gtgatacaat aatatagaag taataaaaaa atagttaata taaaagtgat
     8161 acaataatat agaagtaata aaaaaaaata attaatataa aaatgtgtac aaactaatga
     8221 atattgatat agtaagctat catatgaaag agtatgtaca catttttttt actaattttt
     8281 cacaatatga acataaataa tttacaggaa aggaatcttt tatgaaaatt caaaatactg
     8341 agtggggata tattgaatgg aaacatactt atgatgaaaa taatccaaaa caagctatga
     8401 atatatacat tgcagtaact atgcctggta agaaacattt taatcatgtt cattatggtc
     8461 aagagcagat gatatatatt ttagagggag agggtttgta tattataaat ggagtttgga
     8521 agccatttta tcaaggtatg attttttata tagaatctgg tagcacacat gaaactataa
     8581 atacaggtga tagagaaata aaagaactta ttgtttcaaa taatgtagat gatgtaggag
     8641 agagtgaagt tatagatatt aatcctaata attatttaaa gaagaccttg ataaattata
     8701 gtgaaagtac attaaatttg tatgcagcag tagaatcaat tcgtggtcaa tttatagacc
     8761 cgtttaaaat tccattaatt atatatgatg attcatggaa tatagtttta aaaaatcctt
     8821 attttccact tttttgtttt gaaaaatgta atcctatgaa atttcctcaa aattgtgatt
     8881 gtatgaatca aaaaagtagc aatcaatttg tatgtgaata tgggataact atatataata
     8941 taccaatact gtacaaaagc aatagcatag gtgttataag aggagggtat gtactattat
     9001 ctgatttgaa tttagataca gagcataata atttatatga tattccagag ggagctgcta
     9061 gaagtataaa acgcctcttg aaacaaatat ctaaaaatat aataaatttt tgctctttta
     9121 atgatataag aaaggattta caagaaaaag agaagactat agcaagaaca tatcattatg
     9181 gagagcaact tgaaatgaat ttaaaagtag ctcaagatat ggttactaat ttaagaataa
     9241 atcatcactt tttatttaat acattaaatt caatggcaag catagcacta gatgatggta
     9301 gttatgattt gtatagtgca ataatagatt tatcaagaat gtttagatat acaatgaggt
     9361 ctgatttaag atttgtagaa ttagaatctg aaatattata cattaaaaat tatttgaatt
     9421 tacagaaatt acgttatgga gatgcactta aagtaaaata tcttattcca gagaagttat
     9481 ataacctttc tgtgcctttt aactttattc aacctattgt agagaatgca tttacacatg
     9541 gatttagaga tattgacaca gaaaagcgaa ttgaaataat tgcaagatta gattctcagt
     9601 atgctattat agagattcat aataatggaa ctatcttaga tggaaataat atagacaaaa
     9661 taaaggcagg tattcgtagt aataatggtc atggtttgtc tttaatatat acaaaattca
     9721 catctgctta tggaaataat tttgatatgg atataaagtc gagtgataat gaaggaactt
     9781 acataatgat taaaattcct attgaaaatt ataaggagta tgtatagaac ttgtgaaaaa
     9841 ttaataagtt ttataaatgt taagaaatat attagatgtt tttatgttgt tttatatatc
     9901 ttactttttt atattattaa gctaaaaggg aggtattctc atgagaagag cactaataat
     9961 agatgatgaa gaagctgttc taaaaataat aacacatttt atagaaatga aagatatgcc
    10021 aataaaaatt gttggtaaag caacatctgg tgatgaagca gtagataaaa tcattggttt
    10081 agagccagac atagttttta tagatattca aatgccaata tacaacgggt tagaagtaat
    10141 agaaaaaaca tctcatttat ctaaaaaaat aaactttgta gtaataactg catttaacta
    10201 ttttgaatat gctcaaaaag ctttgagatt aaatgtaaaa gatatactcc ttaaaccatt
    10261 ggatatgaaa gaatttacta agtctgtaga aaaaattatt ggatatcaat ataccaataa
    10321 tgatttatta aatgagatac tggaatatat aaatctaaac tatagcaatg acttaaagtt
    10381 aaatgattgt gctagattat ttttaaccaa tccatctaat atatctagga tttttaaaag
    10441 taatttaaat acaactttta tatcatatct aaatcatata agaatagaaa aatctattga
    10501 gttacttgaa aatactacaa aaagtataaa tgagattgca gaaatagttg gatataatag
    10561 cctaaataac ttttataaaa actttaagat agaaaaagga atgactccaa aagtttataa
    10621 actgaacact aaaaattgaa attgataaat aaaaagtcaa tatagataat tgtttgagtg
    10681 tgtctgaata agtatacttt aattatgata
//
LOCUS       DAYASV010000127        10693 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYASV010000127 DAYASV010000000
VERSION     DAYASV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10693
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.130"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(83..415)
                     /locus_tag="ACGI68_003052"
     CDS             complement(83..415)
                     /locus_tag="ACGI68_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFH0139474.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(659..1300)
                     /locus_tag="ACGI68_003053"
     CDS             complement(659..1300)
                     /locus_tag="ACGI68_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFH0139475.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
     gene            complement(1281..1574)
                     /locus_tag="ACGI68_003054"
     CDS             complement(1281..1574)
                     /locus_tag="ACGI68_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HFH0139476.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            1955..2026
                     /locus_tag="ACGI68_003055"
                     /pseudo
     CDS             1955..2026
                     /locus_tag="ACGI68_003055"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2197..2331)
                     /locus_tag="ACGI68_003056"
     CDS             complement(2197..2331)
                     /locus_tag="ACGI68_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139477.1"
                     /translation="MNISRRAMKIIELAQKIANKRGVTVQDAWNDAMKEYKEKYEYVA
                     "
     gene            complement(2542..3294)
                     /locus_tag="ACGI68_003057"
     CDS             complement(2542..3294)
                     /locus_tag="ACGI68_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFH0139478.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VVMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3353..5008)
                     /locus_tag="ACGI68_003058"
     CDS             complement(3353..5008)
                     /locus_tag="ACGI68_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139479.1"
                     /translation="MEYFSESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     YQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKQCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIDIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSISDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6281..7360)
                     /locus_tag="ACGI68_003059"
     CDS             complement(6281..7360)
                     /locus_tag="ACGI68_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0139480.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIFNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7444..8100)
                     /locus_tag="ACGI68_003060"
     CDS             complement(7444..8100)
                     /locus_tag="ACGI68_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139481.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8422..8628
                     /locus_tag="ACGI68_003061"
     CDS             8422..8628
                     /locus_tag="ACGI68_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139482.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCLAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8781..9209)
                     /locus_tag="ACGI68_003062"
     CDS             complement(8781..9209)
                     /locus_tag="ACGI68_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFH0139483.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9685..10623)
                     /locus_tag="ACGI68_003063"
     CDS             complement(9685..10623)
                     /locus_tag="ACGI68_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HFH0139484.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3544 a         1906 c         1132 g         4111 t
ORIGIN      
        1 agtgaactta gaaaataaag cttaatttca aagacttaaa tagactatta ttattagtct
       61 aaataaacta cgtctatcaa aattatatat taaattcttc taaaattttt attgcaaatc
      121 ttgcgtttgg atatgtaata gacccgccac caattacacc tatttttata gcttctataa
      181 tttcatcttt acttgctcca gaattaatac aaccctcaat atgataacaa atacactcat
      241 cacatcggct taaaattgaa atagctagtc ccattaattc cttatttttt ttgcttatag
      301 ccccttctga ataagtctta tcatcaagac ttccaaatac tctaaaaaac tcatctgttt
      361 cgcttaattt tttattgtaa aatcccctat attctttcat ttctctagta ttcatcttct
      421 acctcctatg tttttcttat aagaatatca caccgtactt atattttcaa tattaaatac
      481 aaattttcat tttatgttca ttagcctaat ttatattatt ttttgaaatc tcaataagtt
      541 atcatataag taagattttt tcagaactaa aatagaaatc ttagtaattt ctttattatc
      601 tatctcagtt ctgaaatata ttattcttat ccaaacatta ttatagtgat taattatatt
      661 agttattttt acctgaaatt ttatacatca aataagaata tataaatggt agtattacaa
      721 ttacaaagat tacaaagata ttataaaaag gaatattaat aaatgcatta ataattaata
      781 atattccacc aataagccaa atccatccag ctaaatggtg tgtcttattc caattatctt
      841 catcattaag agtccatggt aaaattattc ctactgagta atttcgttta catttaggaa
      901 gatagtttcc aaataaaata actattattc ctaaaattaa tcctgtataa aaattagact
      961 ccaaactaac tcctgttgaa tataaaacca tcattatttg tataattact gaaagtatag
     1021 caactcccca ttttgataaa ttttgtataa catttgaaaa attagctttc ttaggctcat
     1081 tctcaagtct aaaccatgta tacaaatgga tacatagcat taacaatggt acaccaaaag
     1141 ctgctataac ctttggagca taattatctg gatttccata attatcaaaa tgaattgcaa
     1201 cttcatttgg taatctatta taaaaatata ttgatacaat aaatggtata aaacatatta
     1261 aagtagataa aataaattgt ttatttatcg cttttttcat tatctttttc tcccttctcc
     1321 ataaattgtg aaaaccaaag catgacctct tcgaaaactg aaatatttat ttcgtagaaa
     1381 ataaagttct tatactttgt ttcaaatatg agtcctgcct tttttagttg agatagatga
     1441 taagaaattg tagcattcgt catattaaat ttttcactaa tttctcctgc tgacatttta
     1501 ttctccctca acataaccaa tatttctctt cttatagggt ctgaaagagc tttgaatgtt
     1561 tctggaaacc ccaacaccat cacctcgcta tttagaattt catctaaata gaatataaca
     1621 taacatccat tttatttcaa tagctattta gaaatttttc taaatagtta tttctaaata
     1681 tttattgaaa atttttatat taagattctc aatttactta agtcgctttt atttaatctt
     1741 actttatcta atttttttaa cttaacaaaa gtgtattgca ccataattgt taaagcaaaa
     1801 acctaatgat tgaggtactt atatatataa caatactttt aattttaaat taaaagtagc
     1861 taaacccaat ttaaaaagta gttaagtcct atttaaatag aaaagaatga aacgagaaat
     1921 atactgtata aaagtacata aaaacatcta ctaattagat acaactataa cagaatatat
     1981 ttattattat aataacaaaa gaataaaaaa aattaattta gcataacctt gttaaatata
     2041 gatttgaata tgaatttaaa gttatgtaaa attaaaaata caggaatgaa aattccattc
     2101 ctgtataaaa tctaactttt gagtctcagt acaatcttat tttgagacaa tcttttttgt
     2161 tttatatttt cttatattta aaatataacg catatcttat gcaacatatt catatttttc
     2221 tttatattct ttcatcgcat cattccaagc gtcttgtact gttacacctc ttttattagc
     2281 aattttttgt gccagttcaa ttatcttcat agctcttctt gatatgttca taaacattcc
     2341 tcctacaaat taccatatat ttcatatata taaatcaagg ttatattttt atgatatata
     2401 atccttctat tgtaagatta cagtttatat gtgtattata tatgaactat attagaagat
     2461 aattagaatt tttataaagc tatatgatta gattaaatat aaaagcttta caattactta
     2521 taaatttaaa aagtctataa gctaaactaa tgtttgatat accaatttta cgaccataaa
     2581 taatgccatt actaaaaata ttggttttat catctttgaa cctttcttta tagcaacttt
     2641 agcacctaca taagctccca ttatcatcac taatgcaaac actatagcaa ttttataatc
     2701 tacttgacca tttatcatga ataataacaa tgaagttata ttacttgtta agtttaatat
     2761 ttttgtattt gcagaagcat gtaaaaaatc atatccatat atttttataa atccaaaagt
     2821 caaaaaagtt cctgtacctg gaccaaaaaa accatcataa aaacccatta taagagccat
     2881 aagcatccct aatcttaaat ttttcttatt aacagcttca aaattatctt cttgacctaa
     2941 atttttagaa ataaaggtgt aaatagccac tattaaaatc ataaccacaa ccaaaacatt
     3001 taatatctct tcacttattg ataatacaca tctcacacct agaacactac caataatagt
     3061 aaatggtact aattttttta aaaggttgtt attaattttt ccagactttg catatctata
     3121 ggcactactt atacatcctg ctgatgctgc aaatttattt gtaccaatgg caagatgtac
     3181 tggcactcca attgccataa gaacaggcat gcttataagt cctcctcctc ctgctataga
     3241 atctacaaaa gcagctaaaa acccccctat acataaaaaa ataactgaaa acataaaatt
     3301 cctccatcat tctctttcaa tttttcttac aatatatatt atataatctt atttaatact
     3361 tattatccag catattcttt ctatattcat ttggagttat actattaaac ttcttaaata
     3421 tttgtgaaaa ataactactg ttattaaaac ctactttctt acaaatatca ctaatactca
     3481 tgcttctatc taacagatat tttttagatt cttctatcct aagaagattt atatactcag
     3541 ttattgaaac tttatttctt tcattaaaaa tagaactcaa ataatttgga cttataaaaa
     3601 aaacatctga tatactattc aaatttatat tttctttata atattttttt atatattcgt
     3661 ttatattttc aattagattt cctttatctt ttctccaaaa tgctctattc ttatcaatac
     3721 actctttgga aaattcaaat aatacattcc ataaatcttc atcactgttt acaagtgata
     3781 tttttttatt atatttattt cttaaataag atataagttt taaatcctct aaatctctaa
     3841 gcactgttct agacaataca aatatcattt cctgaatagt atctctaaca tcattatatc
     3901 ttaatttgtt tgtatctatt agattataaa caccttctaa attcatattt ttaatttctt
     3961 tttcaatata aagttctaat tcaaagggat attcttttga agaattattg aacatatcta
     4021 tcggattaat aatatactct tcttctaata atctactatt tgctttttta agtgcctctt
     4081 caagtcttat attttctttt ctactcttca atatctcctc atcttttata ctaagttgat
     4141 tttcaaaaaa attcctctta gctatttcct gaataaattc agacatcatt aataaatttt
     4201 cttttaatac atataatcta cttttaggat ttaatggaaa ttcattgtaa attttgttta
     4261 tgtattttat atcaatacca gaactacttg ataattcaga taaattttta tctatatcaa
     4321 tagacttata tactataaaa ttaccacatt ctatatatcc aactatattt tcttctaata
     4381 ctataggtat ctctaaactg gaaacaccat actcacaatg gtacactttt tcaaagttat
     4441 cattatttat gcttttaaca agttttttag tacactgttt tatattttta caaatatcgc
     4501 aaaatttatt ttctttctta gtctcaaata tttctttata ctttaagtca tatacagata
     4561 ttctaagctt caatatatta gatatttgat ttgcaagatt ttcaattatg tttacattaa
     4621 ctaactcctg tatattgttt tcagaaaata ctataggtat ttgtctcaaa ctcgaaaatt
     4681 tatgaggaac ataagtaatt aatattacta aatcaatttt accagtgttt ataatttcat
     4741 gttctgaata tggtgggcaa taaactatag atgtttcctt tatattgacc tttttaccat
     4801 caattatttg tattccttct ccctgaatca catataaaag ctgttcttca cttaaatgaa
     4861 aatgcttttc ttggtagttt cccggaaaaa atcttacaat tccagcacta agtctattga
     4921 attgtgtttt tgatggttcg tgtatccaca taacctctcc ccaatcaaat ttttgtactt
     4981 cattatttga ctcactaaaa tactccataa gctcacctct tagtttttgt ctaaatattt
     5041 tttcttccct tcttatatta aaataatatc atcatcatta actttcatta ctataaaatc
     5101 atcatactct tttaatactc ctgtaatttt agttgcactt agtttactac aattcagttt
     5161 tttaatatat tgataaattc tataattact tttttatttt gtaaaatcag tgttttccca
     5221 aaataattat atctataaat gaaagtattt ttactgctta catcaatata tgttatcata
     5281 attttccttt ttattgcata tataaataac tgtatataac attgtccaat actatataga
     5341 aatacatcta agaaagaata ttattaaatt gttccaatta gtattatttt tcttatagaa
     5401 ataaaaaaac tgttggagtc ttttagaaaa attatttttt acataccttt tttgcttaga
     5461 tagtctatct tcacagctat tttgtatata cactcttcta gttcatgatt ttattttaat
     5521 atcttgctta tattgctgaa ataattgaat tcttttatct atcatattgt ctcttatatc
     5581 acaaacatat tgtttcttat atcacaaaaa tgaaagcatt cttatatctt caatatataa
     5641 acctgctttt tttaatcttg taataaatat aggatactct atattctctt cctagaaata
     5701 actaatacca cttatttttc tattatatag aaagtaataa atctttttct taataaaatt
     5761 caatagtaag tggaataaaa ttttatatga ataagaacat tataaaaata ctttatacaa
     5821 tagtcaataa aagatattag cttcaaatta tgttaaagtt gtattattta aagtgaaaaa
     5881 acaaataaaa aagaaataat agtataagta gaaaaatata cttaaaacaa ggcgaagcct
     5941 ctcttgtatc taaatcaagt agagaattaa atctaaaaaa gaaaataaat ataaaagatg
     6001 actcctgtac attacaagaa tcatcttatg gtttcataat cactccatgg tttttaactg
     6061 tctgatataa aggtctcagt tcactcatag catgaccacc ttttccgatt gctgaccccg
     6121 tcacttatgg acaaaatgtc cataagtgat agtcctctct taaaaccctc taaatagata
     6181 tatctcaagc accgcattga aaccagttgc tggaaacaaa ctgaagcgtt ctggaatagt
     6241 aaaagaaatt gccaccatat ccacgttact gtcctagttg ttactttgct aacttcggag
     6301 cgatttcctc taacaatacg cagtcaaatt ctgccttttt gctaagaaat ggcagatggt
     6361 ctgcatctgg tataaaaaaa atctgtttgt ctggcgccac tatttcatca aaaaatttct
     6421 tcgctggaag ataggggcta ttatagtcat tttctcccat gatgtagtat acagggacct
     6481 gatagtcaac tccataattt cttatgtcag cttcttccac taaaaaccgc aatacatcac
     6541 cttgattgtc aatcgcattt ggattgaaaa tggccgtatt ttttaactct ctcaatgaat
     6601 aatacggggt tgccagtata ctgattatat tgcccaaatc catatttata gaaagcccat
     6661 acttgctttg gtatgttctt actgtcataa gagctgccaa atagcttgga ccatattttg
     6721 ctggcggata tggtgcaata gcttccagcg ccttgatatc tttttgcttc cctgcagctt
     6781 tcaccatttc aagtgtcttt tcatagccca cgcgctcatt gtccaccata ttaacggtct
     6841 gcgcaatccc aatgtacgcg ctgtaatcct ccggataggt ttgcacagcc atagttccca
     6901 gaactgttcc ccagctatga cctataagga taattttctc cttgttgaac tcttgcttca
     6961 aatatgctgt cacctcatgg acgtcttgca gaacacgctc cgcgctcatg gtctgcaaaa
     7021 ctgctgccgg atcgttcgca aagtatgttt tgcctacgtt tcgctgatcc cagttcacca
     7081 cagtaaaatc attttcccac tcatactggt acagatgtaa aaatggcatc attgaattac
     7141 caggaccccc atgaacccaa aggatgatgg ggttgtctgc gttttgtccc cggatatata
     7201 acgcctgctg tatgccgccg atctccacca tttccatgcg gtcgattcca ggagcttcta
     7261 ttttatggct tgtacgtata atcgtggggg ctatcagacg caaaccggca aacgccagaa
     7321 caaaaacagc aactagtatg caaagtgtga ttaatatcat tttaagtacc ttctttcatg
     7381 aattttttta gccatagata tctcctctaa cattaaaaaa aatatgttgc tattttagtt
     7441 ttgttactct acctgtccca ttggtttcct ttcataaaaa atattaattg attctataat
     7501 ctcatcttcc aggatatcaa ctcctatttg tctcataaga aactgtaaga attgaaaccg
     7561 tttacgaagt gtaggaaagt ccccctgaaa tacatctgta tcacaccaaa agttaaagag
     7621 cagcaaaatt acctctgcgc attcatctgg gaactctgtg atgatgcttc tatcctctat
     7681 gccttctcgt aaaacatttg ctaatattgg tgctagtttt ttcaaattat cctgtgtaaa
     7741 tgccagtatg atatgcggac tttctatcgc gcttgtaatc atgttactag attcttttat
     7801 cacctcatcc gttaaatgcc gcctaactaa acttctaagc ttctcttttg ctgtaagtcc
     7861 atgcatttca ccgagccatt gatttactgt ctctattatc tgttcaaagc gcctttccat
     7921 gaccacatta aaaatatctt cttttgaact gaagtgatga aaaattccgc cctttgacat
     7981 tcctgaagca tcaacaatat tttgcatact tgttttgtca tatccttttt cagcaaacaa
     8041 ctttgcagaa atggtaatga tattttcaat agcttgcttt ggattgttct ttctagtcat
     8101 tatataactc cttcttatta aaaaccgact gttggttttt attatataag ctattcctgc
     8161 ttctgtcaag taacattgtg tttatggcaa aacaataagg tttggtggtt agacaggtat
     8221 taattagact aatgcgactt tcccctagat ggaacccttg gtaaatttgc aatctttacc
     8281 ggctttgatt atatctgtaa gcaaggcagg tatggccaca cctgccctgt ttgcccctcg
     8341 gatattcatt cctatccctc ctctccttag ccattacacc aaaacgccgc tcattgtctc
     8401 caatggcggc gttttaaaga tatgatttct catgctgctg aaatatgcaa aaaatataag
     8461 atgcccttta ttatttttcc tacaatagaa aagaaaagta ttgaagaatg ggttaatatt
     8521 ggagcaaata tgctttgttt ggcacaagat actaccttta tcacttatgt tatcaatcaa
     8581 ttattatctt tttataatga gattgtacca attcaaaaac aacattaaat tataaatcta
     8641 attttatatt aaaagagact aatattttat ctatctctat taaggatatt ttcttatgta
     8701 tcataatatt agtctctctt attattatat actgctatca tcattagttg ctatttatac
     8761 tatttttata atatacaaat ttattcagct aacgttttgc ctaattctat acactcatct
     8821 tttgcttctt catttgggtc ttccattgct gtaattcctt ctccaccaat aattgtagca
     8881 ccagcatttt gcatacgttc ttcccagtct ctcatccatt cacaatttcc ccatccatat
     8941 gaaccaaata atccaatctg tttacctgat accatagatt ctaattctga aacaaatggc
     9001 tccatatacc cctcttctag ttgctctgct cccatagatg ggcaacctaa tgcaaattta
     9061 tcttcttctt ttaaatcact tggtttcagt aagctcacat ctaaaacttc tggtgtctta
     9121 ccttttaact tcacaccttc agccacaaaa tttgccattt tttcagtatt tcctgttcca
     9181 ctccaataaa caatatatat tttactcatt tttacctcct gtagtatttt ttaattaatc
     9241 aatagttagt tatagctaac taacatctaa aaaacaaaag atttctttat ctgaaatctc
     9301 tttatttttc taaatataga atatcatggt tgattttgaa attcaatact tattgataat
     9361 atttatcatt tatattttat attcatttta taatatcaaa acactgtatc gtatttctaa
     9421 tctttcatag aaaaatttct aacttatagt acttttgcat ctcttataaa tcttttatat
     9481 gtataagtac atagaaaaac acaccttttc cagatgtgct atctataata tcgtatattt
     9541 atttttcaat tctaactagc taatatgttt ataaatttta tcacttaata cctttataca
     9601 ttgatgtaaa atcttacacc taagtaattt ttattatttg tctcgactct atataatata
     9661 gctattttat catcaaatca aaaattatct tttgctatta ttcttgtatt tataaagatt
     9721 taaatataag tatccatttc cttgttccgt tctaggtatt ccaagcgctc tcgtacattt
     9781 tattaattgt gcatacagtt cagcttcatc taaatctctt tcaaactcct ctcttgccca
     9841 ttctttaatt agtgctaatg agcctgatac gtatggtgca ctcatactgg taccactcat
     9901 aatagcaagt ttattatcca tataagtcga tataatattt cttcctggag ctaccaaatc
     9961 tattgtagta tttgaattac taaacttttc aaccaaatag ttctcattta ttgcacctac
    10021 ttctattacc tcagcataac tggctggata actatactca cttgtactag aatcaccatc
    10081 tccattatta cctgctgcac acactacaga aatattattt tttactgctt gcatgacagc
    10141 attttttaaa ttcttatcat ctttgtttcc cccaagagac atagatataa tatcaacctt
    10201 gttatttaca gcaaagttaa tagcattaat tatactttga tatgttccag taccatcttt
    10261 atttaatgct tttgctatta ataatttaca atctggagct actcccataa cttcattatt
    10321 ataattagat gcagctataa tacccgccac atgagttcca tgaccattaa aatcctcata
    10381 tatattttta tttccattac tgtcatcact aaaatttgca ccaccaatta tttttccttt
    10441 taaaagagga tgagatatat cacaacctgt atctattata ccaactacaa tatttttacc
    10501 agtataacct tcatcccaca ttcccctagc attcatttgt tttattccat atgggaattt
    10561 attttttgca cctcttagat tttcatttat ttcatatgga attagtttca ttcttttatt
    10621 catcatatat cctcctaaaa attttattgc ttataaaaat catgaatttt attttataat
    10681 aggtttataa atc
//
LOCUS       DAYASV010000128        10639 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYASV010000128 DAYASV010000000
VERSION     DAYASV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.131"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..321
                     /locus_tag="ACGI68_003064"
     CDS             <1..321
                     /locus_tag="ACGI68_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0139485.1"
                     /translation="VEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDS
                     EITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKS
                     VYGK"
     gene            454..1824
                     /gene="hydA"
                     /locus_tag="ACGI68_003065"
     CDS             454..1824
                     /gene="hydA"
                     /locus_tag="ACGI68_003065"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HFH0139486.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEKIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKIAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISVNKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            1842..3248
                     /locus_tag="ACGI68_003066"
     CDS             1842..3248
                     /locus_tag="ACGI68_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139487.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     IRGTIKNRMEGHKWNSLY"
     gene            3320..3766
                     /locus_tag="ACGI68_003067"
     CDS             3320..3766
                     /locus_tag="ACGI68_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFH0139488.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            3805..4599
                     /locus_tag="ACGI68_003068"
     CDS             3805..4599
                     /locus_tag="ACGI68_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0139489.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMTKALVVRMYNSIGGECDGK"
     gene            4589..6880
                     /locus_tag="ACGI68_003069"
     CDS             4589..6880
                     /locus_tag="ACGI68_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0139490.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            6881..8194
                     /locus_tag="ACGI68_003070"
     CDS             6881..8194
                     /locus_tag="ACGI68_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139491.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            8230..9315
                     /locus_tag="ACGI68_003071"
     CDS             8230..9315
                     /locus_tag="ACGI68_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFH0139492.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASIGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            9551..10594
                     /locus_tag="ACGI68_003072"
     CDS             9551..10594
                     /locus_tag="ACGI68_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139493.1"
                     /translation="MQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEVKQ
                     EMTVMKEDMSEVKQEINVLKEDMSEMKQEMTVMKEDMSEVKQEINVLKGDMSEVKQEM
                     IVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEMTVMKEDMSEVKQEINV
                     LKEDMSEVRQEINIMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMK
                     EDVSEVKSEVNFMQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEI
                     QIEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATS
                     KNWSDIVKLKSVK"
BASE COUNT         4283 a         1117 c         2169 g         3070 t
ORIGIN      
        1 gttgaaaaag ctgattgcta tacaggaaat actatagaaa gtgaaagatt cttcccaggt
       61 tggttatatg acgaagaaga tgaatttgtt caagcagcgt ataagggatt aaaagaagct
      121 ggaatagatt cagaaataac tcaatactca ttctgtacga atggaagtca ctatgcaggt
      181 gaagctggaa taaaaactat aggatttggt ccttctaagg aaaatcttgc tcacacaata
      241 gatgagtata tagagcaaga acagttattt ataggaacag aaggttacta tggaatatta
      301 aaatctgtat acggaaaata atatttagtt cacaataaat tatttaaata tagtctagag
      361 gtgctgaata tagcaccttt agaaaataca attttaaata aagttttaca atacttagaa
      421 aatactttaa agcttaaaag ttaggaggta tatatgggaa caatactaag aggtggaact
      481 ataattacct ctgataagac ttatataagt gatttaagga tagaagatga aaagatagtt
      541 gaaataggaa ataacttaga aataaatggt gataaggtta ttgatgctac aggtaaaatt
      601 gtaattcctg gtggaataga cacacatact cattttgata tgaatgcagg ttctattaca
      661 actgcagata attttgaaac aggaacaaga gcagctatag ctggaggaac tactacaata
      721 ctagattttg cagaagccaa tgagggggaa aatctactgc aaggtgtaga agcatatcat
      781 aaaaaagcaa gtggaaattg ttattgtgat tatggttttc atatgacaat tacatgttta
      841 aataaagata catttaatca tatggaaaaa cttatttcag atgggatagt atcttttaag
      901 atgtatatgg catatgatgg catgaaagta gatgatggaa caatatacag ggttttaaag
      961 aaagcaaggg atttaggttg tatagttgga tttcattgtg aaaatgggga tttactagat
     1021 gtattgatag aagaaaatat acaaaatggt aatttggagc caaaatatca tccacttaca
     1081 agacctaaca tagttgagaa agagtctgtt tcaagacttg ctgatataac taaattatct
     1141 aattcaaaat cctatgtagt ccacttaagt tgtagggagt cacttgaaac tgtaaaaata
     1201 gctagagaaa aaaatataga tatgatagta gaaacatgcc cacagtatct attattagaa
     1261 gacaatttat ataataagga taaatttgag ggtgctaagt atgtaatgtc tcctccttta
     1321 agaaaaaaag aagacataaa ttacctttgg gaaggattgt ctagtggaga tattcaaaca
     1381 gttggcacag accactgttc atttaatttt aaggggcaaa aagacttggg aattgatgat
     1441 ttcagtaaga taccaaatgg agcacctggt gttgaacata ggttagcact tttatataca
     1501 tatggggtct tagaaaatag aataagtgta aataagtttg tggaagttac ttctacgaat
     1561 gctgcaaaaa tatttggaat gtatcctaaa aaaggtgaga tagctgtagg aagtgatgca
     1621 gatatagtca ttttaaatat caataaagaa gaaacaatta gttataaaac tcaaaaacaa
     1681 aatgtagatt atactccata tgaaggattt aaggttaaat gtaaagttga agatgtattt
     1741 ttgagaggaa atcatgtagt acaaagttgt aatgttaaag aacatccaac aggtcaatat
     1801 attaaaagaa aaattactaa ataatgcggg gggaaataag aatgatggat atacttatta
     1861 agaatgccat aatagtaact gtgaataaag agagagaagt tatttttgat ggggcattag
     1921 tagtaaaaga taacaagatt gctgatattg gaaattctaa agagatagaa agtaaatata
     1981 ctgatgttaa aaaaattata gatgctaaag gtaaagtttt attcccaggt tttataaata
     2041 cacataatca tttattccaa actttattaa aaggattggg agatgatatg gtacttaaag
     2101 actggttaga aactatgaca tttccagcag ctaactattt agagcccaaa gatacttatg
     2161 atgctgcaat gcttggatgt attgaaggac ttagaagtgg tataacaaca atggttgact
     2221 acatgtatcc tcatagtaag ccaggtcttt gtgatggaat aatagatgct tataaagaat
     2281 taggaataag aggaatactt ggaagaggat gtatgaatac aggagctcaa tttggagttc
     2341 atcctggaat aatgcaagat gtagaaacgg ttgaaaaaga tgtaagaaga ttgtttgaaa
     2401 aacaccataa tacagaaaat ggaagaatca aaataggagt agctcctgca gcaatatggt
     2461 caaattctca agaaatgctt gaaatgctat ggagagtagt aaaagaatat gatgatgctt
     2521 tatttacagt acatatatca gagactcctt ttgatagaga agcagcaaaa gaattgcatg
     2581 ggcaatatga catagatgtt ttagaaaaat taggaatact tggaccaaat gtgcttatgg
     2641 tacattgtgt gtatctaact gaaaaagata tggaacttac taaaaaatac gatatgaaag
     2701 tgtctcataa tacagcaagt aatatgtact tatcttcagg tgttgcacca gtgccagaaa
     2761 tgcttaaaaa aggaataaca gttagtttag gagtagatgg agccgctagt aataactctc
     2821 aagatatgct tgaacttatg aaattaacag cacttcaaca taaagttaat aagtgtgacc
     2881 cattagcaat gtcggcagaa aaagttcttg agttggcaac tatagatgga gcaagagcta
     2941 ttggaatgga agatgagata ggttcattgg agataggtaa aaaagcagac ttactaatat
     3001 ttaatccaat gctttcacca aaagcgatac ctatgcataa tccggtatct acattagtat
     3061 attcttctag tatgaaaaat atagagagtg ttatagtaga tggaaatata ataatggaag
     3121 attcaaaaat attgacagct aatgaagaga aagcattaaa agatgctcaa gatacagctg
     3181 agagactttg tataagagga actataaaaa atagaatgga aggtcataaa tggaatagcc
     3241 tttattaata aatgtaattt attcataaaa aagtatagta aaactttagc ttaaaaatat
     3301 gagttttgag gtgatttatt tgattatcaa atgtaccata aataataaag aaaaagaatt
     3361 aagtataaga ccagatgatt ttttagcaga tacattaaga gaaaatggat atgtaagtgt
     3421 taaaaaaggg tgtgatacag gctcatgtgg gttatgtaca gtgatggtaa atgacaaggc
     3481 cgtgctttca tgtaattatc ttactgctag agtagaaggt cagcatatta caacacttga
     3541 gggtgtcttg gatgaagcaa agaagtttgg agattttctt gcagacgaag gggctgacca
     3601 gtgtggattt tgtgctcctg gatttataat gatggtcttg gcaatgaaaa gagagcttca
     3661 aaatccaaca gaagaagaag ttatagatta tgtaaatggt aacctttgta gatgtacagg
     3721 atataaaggt caattaagag caatacataa atatttaaac acttagactt ttaagtgaaa
     3781 attaagttga tatggggaga gagtatgata actataaagg aatatgtagt tccaaaaagt
     3841 ttagatgaag cttatgaact gctaatatca agaaaaaaca acataatact tggaggatgt
     3901 ggatttttaa aactaggtag taaaaatatt ggttcagcaa tagatttaaa agacttagct
     3961 ttagattata taaatgaaac tgatgatagc atattaattg gagcagatac aagtttaaga
     4021 actttagaat taaataaggt tattaaaaat tactgtaatg gggtaatttc aaacgctgtt
     4081 tctaatatag taggagttca atttagaagt ggagctagag taggagctag cgtatttgct
     4141 aagtatggat tttcagattt aataccttca ttactagtgg tagatgcaaa ggttaagtta
     4201 tataaaaaag gggttatgaa cttagctgat tttttagaaa atgagcttga aaaagatata
     4261 ttaatagaag ttatattacc taaaaaagat gctataggtg tatttgattc tataagaaaa
     4321 tgtacaggtg attttgcagt attaaatgga gcaatgttaa aagaaggaag tatttataaa
     4381 attgcgatag gcgctagacc aagaagagca aaaattgcct ataaagcaag tgaaatatta
     4441 agtttagaaa atgatataga aaaagctggt gaagtatcaa gtgaagaact ttcatttgga
     4501 agtaatataa gaggaagtaa agaatacaga aaagatatga caaaagcctt agtagtgaga
     4561 atgtataata gtattggagg tgagtgtgat ggtaaataaa gaggtaagaa aaatagatag
     4621 tgaagccatt gttactggaa aaccaatata tacagaagat ttaatttttc ataaagatgt
     4681 tttaacaatt aagcttctta gaagtccaca tgcttttgcg aagataaaaa acatagatat
     4741 aaaaaatgca ttaaaggtaa aaggtgtagt aaacatattc acttacaaag atgttccaaa
     4801 ttatagatat tcaatggttg gagagtcata tccagaagca tcacctcatg accaattaat
     4861 acttgaagac gtagttagat atgtaggaga tgaagttgct ataatagccg ctgaagatga
     4921 aagggctgct attaaagcta tgaaacttat aaaagttgaa tatgaagtaa tgactcctat
     4981 attggatgca agagaatcgg aaggaaataa aattttaatc catgaagaag atgatttgtt
     5041 ttgtccattt gattttggat tggatgctac aaaaaatatt gtatcgagag aaaaaatggg
     5101 taaaggggat gtagaaaaag agtttaaaga aagtgatgta atagtagaac attcttatat
     5161 gactcaatca caagcccatt gtatgatgga aactcataga gcttatacgt atatagatac
     5221 gaatggcaga ttagtagtaa cttctgcaaa ccaatcagct tatcatatga gaagacaggt
     5281 ttctaaagca ctgggaatac ctctttcaaa agtgagagta ataaaaccaa gaataggtgg
     5341 aggttttggt ggaaaaaaca tagctgtaac agagatatat gcatcatttg taacttggat
     5401 gacaaaaaga ccagcaaaac taatttatac tagagaagaa acttttgcaa tgactaatac
     5461 tagacatcag atgttttttg atataaaaat tggggcagat aaagaaggaa acataaaagc
     5521 tatagatatg aaagcattga acaatacagg cgcttatggc gataatggac cttcagtttg
     5581 ctctgaatca ggtcataatg tacttccaac atataataat gtacctgcaa taaaatttga
     5641 tgggaaaact gtatatacta atttagttcc aggaggagca ttaagaggat atggagcaac
     5701 acaaggtaca tttgcacttg attgtgcaat tgatgaatta gcgcataaac ttaatatgga
     5761 cccaacagaa ataaagttaa agaatattat aaaaaaacaa actgaaggcg gaataatgaa
     5821 ttttccaata agaagttgtg cacttgatga atgtataatt agaggtaaaa aacttattgg
     5881 atgggatgaa aagtaccctg ttagggatat tggaaacaat aaggtgaggg ctgttggaat
     5941 gtctgtggga actcatggtt ctggtatagc caacatagac atggcaatag taaatatgag
     6001 gatggatgaa gatggaactt ataagttgtt ttctgggtct tcagacttag gaactggttc
     6061 tgacacaata cttgcacaaa tagctgcaaa agcattgaat acatctattg atagaatttc
     6121 cgtttataca ggagatacag atatgtgtcc atatgatact ggtgcatacg cttcttgtac
     6181 tacttatgta actggaaatg ctacaataaa atctgctgaa agtttaaaac aaaagatatt
     6241 aaaagtagca cagaagaaac tcgatttacc aatagatgat ttaatactat atgaagacag
     6301 agtttgttat agtgaagatg tagagaagtt tgttttgtta agtgaattag gaaaagaatc
     6361 tgttggtggt ggaaatcaag aagttttata ttcatctgaa tcttatggta tacaagagag
     6421 tgctaaacca ttcttagcag gatttgctga aatagagtta gataaaacaa caggggagtt
     6481 taaagtaata aattatgctt gttctacaga ttgtggtaca gttatgaatc ctgcacttgc
     6541 tagaatacaa gttgaaggtg gtgtaactca aggtataggg cttgcaatgt atgaagaccc
     6601 tagacatggt tctgatggaa agcttcaaac ttctaatcta ttacaataca aagttccaac
     6661 aaaaaaagat ataggaaaca taattgttga ttttgtagat aattatgagc caacaggtcc
     6721 atatggagct aaatctttag gagaaatagt tattcataca cctgcaccag caatagcaaa
     6781 tgcaatatac aatgccacag gagttcgtat aagagaactt cctataactt ctgagaaagt
     6841 ttatatggcc atgaaaaaat taaatcaggg aggtaaataa atgattgcta ttaaaagtaa
     6901 atacttaata tcttctgctg atgaaatata tacaaattct gctgttgtag tagaaaatga
     6961 cattataaaa gatatattgc caaatgaaga agttgaaata aaatataaaa atattgaaga
     7021 aataatagat aaatcagatg ctataataat gccaggattc gtcaatggtc atatgcatca
     7081 atatggagtt ctttctaggg gaataccggc aaatgtacat tttactgact ttgaaggttt
     7141 tttaaatgac tattggtggc catttataga aaatagaatt ggattaaaag aagtgaaggc
     7201 aacaactaaa gcatcagcaa tagaacttat agaatctggt gtggtagctt tttgtgatac
     7261 cctagaagct ccaaatacag aagaaggaac tttaattgag caaggaaaaa tactagaaga
     7321 aataggcatg aaagcgattt tatccttaga aagttgtgaa agaataagct ttgaaaatgg
     7381 acttagatgt ttagatgaaa attctaattt aataaagtgg agtagagaaa atagtaaatt
     7441 aataaatgga ataatgtgta cacatacaag cttcacatgt tctgatagat ttattaagaa
     7501 agctaaagaa gatgcaaaaa aattaaatgc accaatgcaa tttcatttat gtgagagtat
     7561 atatgagcca aactatgctg aaaaacactt tggcaaaaaa gcagttgact actataatga
     7621 tttagatata ttggatgaaa ctgtattagc atctcagtgt gtaaaagtaa atgatgaaga
     7681 aatagatata ttaaaagaaa aaggcgtaaa agtggttcat atgcctctta gtaactgtga
     7741 agtaggtgga ggtttttctc cagtaccaaa aatgattaaa aaaggcataa aagtggcact
     7801 gggtactgat ggatatataa atgatttttt tgaagttatg agaggtgcat ttttaatgca
     7861 taaatcagtt gaagaagatg catcagtaat gccagcaaat ttagtattta gaatggcaac
     7921 agagcatggt gcttatgtat taggacttca aaattctggt aaaatagcag ttggtaataa
     7981 ggctgatata atagttatgg aagatgaatt taaaactcct gtgactcttg ataatatatt
     8041 tgaccagata gtagttcagg gtaaaaaaga atttatatca aatgtataca tagatggtag
     8101 acatatttta aaagaaaaac agttagttga tttagataaa aaagctattg ttaaagaaat
     8161 gaaagaagtt gcttgtgaat tttggaaatt ttaacgaatg taaatatata tgaaaagggg
     8221 aagcagataa tgtataattt tttaggtaaa gaattaaaat caccacttat aataggttca
     8281 ggaccattaa catatagtgc agctggatgc aagatattaa gtgatgcagg agcaggggca
     8341 gttgtgacta agactataag aaaagaaaga gctataaatc cagcaccaca tatggttaga
     8401 aatacagcta atgctctttt aaataatgaa aaatggacag attttgagcc agagcaatgg
     8461 atagattttg aaataccaca aatgaaaaga gatgggactg tctgtatagc ttcaataggt
     8521 catacaattg aagaaagtag cgaattagta gagaaagttg caaatgcagg agctgacttt
     8581 attgaattag tatcttatga ctatagagat ttgataccaa tgcttaaaga tgcaaaggaa
     8641 agagtaaata tacctgtaat agttaaactt cctcctatga tagatgagat aggcgatttt
     8701 gctaaaaaat tagaagaagc aggagcagat gcaataactg cttgtgactc tgtaggacca
     8761 gcatttagaa tagacataga aacagggcaa cctttattag gtggaaatgg aataggatat
     8821 ttaagtggag aaactatcaa gccaataact ttacagagaa tatatgaaat aaaaaaacaa
     8881 gttaatatac caataattgg cttaggtgga tgtgtaagtg gagatgatgc cttagaaatg
     8941 attatggcag gtgctgactt tgttggtata tgttcagtag taattttaaa aggtgcacaa
     9001 gtaatatcta aaatacatga tgatttaaaa tctaatctta atagattagg atataatact
     9061 atagaaaatg cttgtggtat agtacataat catatgggag atgttaatga aagacttagc
     9121 tttgagttta cttatgatga agaaaaatgt actaaatgta agatgtgcga gagagtttgt
     9181 gcatatcaag cgagaaaatt gaatgataag atggaattga atgaagaaga gtgtagatat
     9241 tgtggacttt gtatatcagt ttgtaaacca aaagcacttg gaagaaaaat ttcatgttca
     9301 gagctgaatg tatagattat tttataaaaa acaaagtttt aaataaaaat aagaaaggct
     9361 atctcaaaga gataagcctt ttttgttgtt gaccttattt ttgtttattt gtagttattt
     9421 tagatattaa ttagttttga agtaataaag tgaatcaatt ttgtttaatt aaaattttaa
     9481 taattctgat atatatgtta acattgtata gcatttgtgt aaaatgttat aataattaat
     9541 agggatggtg atgcaagtgc aagagaagat tttagagtta ttatatacta taaatgaaga
     9601 tgtagttagc ataaaaaatg atatgtcgga agtaaaacaa gaaataaatg tgttaaaaga
     9661 agatatgtcg gaagtaaaac aagaaatgac tgtaatgaaa gaagacatgt cggaagtaaa
     9721 acaggaaata aatgtattaa aagaagatat gtcggaaatg aaacaagaaa tgactgtaat
     9781 gaaagaagat atgtcggaag taaaacagga aataaatgta ttaaaaggag atatgtcaga
     9841 agtaaaacag gaaatgattg taatgaaaga agatatgtcc gaggtaagac aagagataaa
     9901 tataatgaaa gaggatatgt ccgaggtaag acaagagata aatacaatga aagaggatat
     9961 gtcggaagta agacaagaaa tgactgtaat gaaagaagat atgtcggaag taaaacagga
    10021 gataaatgta ttaaaagaag atatgtccga agtaagacaa gagataaata taatgaaaga
    10081 agatatgtcc gaagtaagac aagaaatgac cgtaatgaaa gaagatacat cagaggtaaa
    10141 acaagagata aatgtaatga aaaaagacat gtcagaagtg agacaagaaa taactgtaat
    10201 gaaagaagat gtttccgaag ttaaatcaga agtaaacttt atgcaaaata aaataaataa
    10261 cattaatgaa gatatgaatg gaattaagga cgaggtatcc atagcaaatg aaaaattata
    10321 tggaattgaa attaagattg atagtctaga atgtgaagat aaatcaatga aagagataca
    10381 gatagaacag aataatattt taggaagttt attacataat tctgaaataa ataaagctac
    10441 tcatgataat atagaacata acatagcata tataaaaggt gacactaatt ctataaaaga
    10501 ggatatagct gaaataagaa gagacttaaa tttagtagag ttagccacta gtaaaaattg
    10561 gagtgatatt gtcaaactaa aatcagtaaa gtagtatttt aattcactaa tttatcatcc
    10621 acaaactaag aattttata
//
LOCUS       DAYASV010000129        10544 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYASV010000129 DAYASV010000000
VERSION     DAYASV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.132"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            29..1729
                     /locus_tag="ACGI68_003073"
     CDS             29..1729
                     /locus_tag="ACGI68_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139494.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            1899..2516
                     /locus_tag="ACGI68_003074"
     CDS             1899..2516
                     /locus_tag="ACGI68_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139495.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     regulatory      2627..2795
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2877..4070
                     /locus_tag="ACGI68_003075"
     CDS             2877..4070
                     /locus_tag="ACGI68_003075"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFH0139496.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHLVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            4088..5380
                     /gene="lysA"
                     /locus_tag="ACGI68_003076"
     CDS             4088..5380
                     /gene="lysA"
                     /locus_tag="ACGI68_003076"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFH0139497.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            5597..6256
                     /locus_tag="ACGI68_003077"
     CDS             5597..6256
                     /locus_tag="ACGI68_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0139498.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNNNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(6411..7496)
                     /locus_tag="ACGI68_003078"
     CDS             complement(6411..7496)
                     /locus_tag="ACGI68_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0139499.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            7687..8403
                     /locus_tag="ACGI68_003079"
     CDS             7687..8403
                     /locus_tag="ACGI68_003079"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFH0139500.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            8461..9039
                     /locus_tag="ACGI68_003080"
     CDS             8461..9039
                     /locus_tag="ACGI68_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0139501.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            9433..10305
                     /locus_tag="ACGI68_003081"
     CDS             9433..10305
                     /locus_tag="ACGI68_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0139502.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4353 a         1112 c         1838 g         3241 t
ORIGIN      
        1 aataacattt tttagagagg agtattaaat gaaacgatat cttaaaacat atggagttgc
       61 attaataatt gtaataattt atgcatttat taaattacct gtactaagat tagattttac
      121 atctttattt tcagtaggaa taattttctt tgttgtagca ggaatactag atatgatgtt
      181 agatagaaat gaaagagcat ctaaaatggc taaatataat ttttcaatag caatagcttt
      241 gcttgtgtat attgtagtag ttccctttat aacttctaca cctgtacttc atgcaaaatc
      301 atatagagaa ctattgggaa aagtaactga aagtaaattt actgatgaca ttagccctgt
      361 cagtgtaaat gacattcgtc ttgtagatga agatatggca atgaaattag gtgacaaaaa
      421 gcttggagaa gttcctgcaa tagggagtgt atccaaatta ggaaaatttc atatacaaaa
      481 tgtagatgga gagttgtact gggtagcgcc tcttgttcac agagacataa ttaaatggat
      541 aacatctcta agtggtacaa gtggttatgt aaaagtatca gctagtaatc ctcaagacgt
      601 ccaattagtt caagaaatag atggaaagcc aataaagata gtttaccaac cagaagccta
      661 cttacatcag gatttacaaa gacatttata catacatggt attgtaaatg ttggtatgac
      721 agatttcact cttgaaataa atgatgaagg aagaccttat tgggttgtaa gtttatatga
      781 acataaaatt ggctatggag gagcaaatgc aacaggaata gccactgttg atgcagaaac
      841 tggaaaaata aatgtttatg atgttaaaaa cacgcctaaa tgggtggata gaatacaacc
      901 acaaagcttt gtaacagacc aaattaaaga ctggggtgtt tatgttaatg gatttttaaa
      961 ctctgttata tcagaaaagg gagttttagt accaacagaa ggtacatcat tagtttatgg
     1021 caatgataac aggtcttatt ggtatacagg tataacatct tcaggtggag atgaatctac
     1081 aattggattt atgttagttg attctagaac aaaagaagct agattatata aacaaccagg
     1141 agctactgaa acggctgcaa tgaaatcagc tgaaggtaat gttcaagaaa aaaattatga
     1201 agcgacgttc cctgtaatgt ataatatatt gggtcagcca acatatgtat catcattaaa
     1261 agataaggcg ggtcttgtta agagagttgc atttgtatct gttgaagatt ataatgtact
     1321 tggaataggt gaagataaga atgaagcatt aagaaactat aaggatgcat tagaatctaa
     1381 gggtaatgat ttaaaacttg ataatgattt aaaagatgag gtattagaag gcacagtgac
     1441 aagaataagc ccagatgtgc gaggtggtaa cactaattat tatgtaactt tagattcaaa
     1501 taaagatata atatttaggg ctacttcaaa agtatctagc gaattgccat tgactcaggt
     1561 tggtgataaa gtcaaaatat cgtattcaaa agaagaatct ggtgtaatag aaatgtcaga
     1621 atttgataac ctaaatattg caaactttga agataaaaaa gcaaaagaaa aaacaggaac
     1681 tgatgaaaat acagattcca aagattctga aaatcaaggt aatgtataat ttaaataaaa
     1741 agattgataa tagtatttaa catagtgata gctaattttt atagaattta attctataaa
     1801 aattagctat tttattttaa ttctttgtaa atttaatgtc ataaaattaa tataatatta
     1861 gtacaacttt gcaaataata cttcaaggag aatgtactat gacgaatatg gaaaaagaga
     1921 tattactttt aattagcagg gttagatgtg ttacagagtc tcaatttaga aaaatttatg
     1981 gagaccaaaa aagatacaat aaaaaaaatt ttaaaaaaac tttgagaaaa atgtgtagtg
     2041 aatatacact aaaaaaatat ccttgtgatg tggattatta taattataaa gacctctctt
     2101 atgtatacta tttaaacggt agtaaacttt ttaagggaga tgatttaata aaggctctag
     2161 taggatcaga attagttgtt agaatcaata ctgcaggttg tgatgtaaaa agattttacc
     2221 gaaatgttag tgtagatgat gtgaaatacg atttatttat tgaatataca gaccaatttg
     2281 gtaatataaa gcaagtttta gttgatgttg acttaaataa tgaaatagac atttctaaat
     2341 acgaaaatct ttctaaaaaa atagataaat ctacaattcc attttttaaa gtaccaaacg
     2401 ttttggtagt tacaccagat aatctatccg aattaaatga tttagagaga tatgataatg
     2461 agtatagcat aaattttata gatttaggat taaataaact atttaattgt atataagcta
     2521 actttagaaa agaaaactat cgatttgcca cattttgtaa aataattgtt gcaatattat
     2581 atagaatatg ataaaactat attaataaat taattaaaaa taatgagcct agggtagagg
     2641 tagctgtatt caatagtatt caataggagc tggtaagcgt tgataattga tgaaagggta
     2701 tatagccgaa gaaattttgt tgacggacaa attttctgga cttatgcaga atatgcataa
     2761 ggctgtcact tttaaagtgg tgagctacaa ggttacttgg tctatgaaat ttacacgtcc
     2821 aaagtatacc tttgggcgtt tttatttatc aaaatttagc ttgaggggga gaaagcatga
     2881 gtataattgt tcaaaaatat ggtggaagtt cagtagcaga tactgaaaaa ataaaatcaa
     2941 tagcagaaaa tataatagaa cgaagaaaag aaaatccaca aatggtaata gttgtatctg
     3001 ccatgggaaa atccactgat gaatatataa ctttagcaaa ggaattgagt aatgaaccta
     3061 gtaagaggga attagatgct ttaatgtcta ctggtgaaat gatttcagca tctttattat
     3121 caatagcatt aaatgcactt ggatgtaaag ctataagtta taatgcttac cagttaaata
     3181 taaaaacgag tggtcttcat ggaaagtctc aaattgatga tataaacgta aacagaatta
     3241 aaaatagttt agatgaagga aatgtcgtga tagtaactgg atttcaggga atcaacgaag
     3301 atggtgatgt aaccactctt ggaagaggtg gttcagatac atctgctgta gctcttgcgg
     3361 ttaaattaaa tggaaaatgt gagatatata cagatgttga tggaatttat tttacagacc
     3421 caagaaaata ttcaaaggct agtaaacttg atgagattga atatgaagaa atgcttgagt
     3481 tagcgagttt aggagcacaa gtaatgcatt caagaagtat agagcttgca caaaaatatg
     3541 gtgttgaaat ctatgtcgga cgtacatgtg gtacagaaaa aggaacatat ataagagggg
     3601 gaaaagacat gaaattagag gataaagtaa taacaggatt agctactagt gatgatgaca
     3661 gttctataac aataaaagat tttaaagctg aaaatatatc tagcttattt gaagatatag
     3721 caactatagg tataagtgta gatatgataa gccaaactgc acctatttta gataagataa
     3781 gtgtatcatt tacagttcca aaagaagagt tgggagaatg taaaaagata gtatctaaat
     3841 atacagatga agaacattta gtgattgata ataatatcac taaattttct ttagtaggtc
     3901 ttggtatgaa aaatacttct ggagttgctg ctaaagtatt taagatattc aatgaaaatg
     3961 gtataatgat aaaacttata actacatcag agattcgtat aacttgtgct ataaattctg
     4021 atgataagca agtagctata gaaaaaatag cagaagtatt taatatataa aatttgggga
     4081 ggctattatg aaacttcatg gaactatgac tgtaaataat gatgaattat atattggagg
     4141 agtgagttgt ttagaactta gtaaaaaata tcaaactcca ctttatgtat ttgatgaagc
     4201 tttagttaga caaaattgta gagaatatat agaatatttt aaggctagag agggaaaaaa
     4261 taaggttgct tatgcaggga aagctttttt acctctttac atgtgtaatc ttataaatga
     4321 agaacaaatg tgtttagatg tagtttcagg aggcgagtta tatacagctt ataaatcaaa
     4381 ttttcctatg gaaaaagttt tctttcatgg aaacaataag actaaagaag aaataaacat
     4441 gggattagat ttaggtgtag gtaaatttgt agtggataat ttttatgaat tagatttgct
     4501 tgaagcatta tgtgaagata aaggtaaaac tcaagagata tactttagaa taactcctgg
     4561 tatagaagct catacacatg attatataaa aactggacag atagattcta aatttggatt
     4621 tgctttagta aatggagatt tatttagagc aatagaaaaa ttaagtgaat ataaaaatat
     4681 taaacttgta ggtcttcacg ctcatatagg ttcacaaata tttgatgtag aaccatacat
     4741 agatacagtg gatataatga tgaacttggt aaaagaaata aaagaaaaat tcaacataca
     4801 actaacagaa attgacctag gtggaggagt tggcgtttat tatagcgaag gagataaacc
     4861 taaaactata aaagagtttt gtgaaaccat aatcactaaa gcagaaactt catgtaaaga
     4921 gcttggaata gaagtaccta cattggtaat agaaccagga aggtcaatag ttgcaaatgc
     4981 aggaagtaca ttgtatacag taggttctat aaaagatata aaagatgtta gggtttatgt
     5041 aagtgtagat ggaggtatga cagataatgt aagaccttcg ttgtaccaag ctagttatga
     5101 gtgtagtatt gtgaataaaa ttaatcatga ggctatgaat catgtaacaa tagctggaaa
     5161 atgttgtgag agtggagata tattaattgg agatataaac ataatggata ttaaaagtgg
     5221 agatatactt ataacaacat caactggtgc atatggatat tcaatgtctt caaattataa
     5281 taaaatacca agagcagcag tggtatcagt atttgatgga aatgataaga taatttgtaa
     5341 gagacaaagc tatgaggact tattagcttt agagatataa aatagagaag tatataataa
     5401 aaattaaata ttaaagttta ttatgagcag atttgctagg atatgtatct ctaaattaat
     5461 ttttggttta ttgtatcgtt cgtagcatat ctgcttattt tttgttttac taacaaattt
     5521 cttgtaataa atataaaata ttttaaaaac tttataatta tgataaaatt taaagtatat
     5581 gacaaggagg gatttaatga gaggaagagt aattttatta atatcgatat tatctatatt
     5641 tcttgtagga tgttcattta ataaaaaaga tgaaataaat ttagtagagc aaggaaaaac
     5701 atatttagaa aaacataact ataaaaaagc tatggaatca ttatcctctg cactggaaga
     5761 agatagtact aatgagaatg caagagctat gtatatgcaa gctatgagaa tgtcaaatat
     5821 gacggagttt gaagaattaa aaaattatga gggtgcaatc aaggaactta aacttataga
     5881 gaaaatcaaa aatggttcct ctgttattaa agaagaggca actaaaaaga aagaagagtt
     5941 aactaaatta gaaaaagaac aaaacgaagc agaggaacaa agaaagaaaa aggcaaaaga
     6001 tacggctgga gaagataggt atagagttga atcagatgca aggaaatctt cttatagtgg
     6061 aagtagtaaa aataataaat ctagttctaa aaacaatgat aaaaaatcag atagtaataa
     6121 gtctgacaat aataacagtt cttctcaaaa tcaagaaaag ccaaataata gtaatccaac
     6181 accagcacca actccacaag atactactgg tggttcagat agtggtggaa attctcaaaa
     6241 ctcaaataat caataagtat aaaatatatg cataaattta atgaacaaat tatatattat
     6301 aaaaaatatc tcttagcaat tttttcttac taagagatat tttttgagaa ataatttaat
     6361 aaatagcagt taaattttaa actaaaaatt aaagtcacag tagagataat ctattgtaaa
     6421 cgattatcta tatatttatc aacataagtt tttacaacag cagctattgg tactgaaaga
     6481 aaaactccta tgaaaccaaa tagagattgt cccaccaaaa tagcagatat tacccatata
     6541 gctcctatac caacttgatt acccataact tttggttcta taaaatttcc atctaattgt
     6601 tgcaatataa atagaaatgc aatgacccat atagtttttt gtggactata taatatagtc
     6661 atggctattg gagggattgc accaataaaa ggaccaaaat agggaattat attacctaag
     6721 aatattattg aagctaaaaa taaagcattt tctattctga taacaaattg aaatcctaaa
     6781 aatgcaatta taccaataat aagagagtct aatattctac caagtaaata atcatagaaa
     6841 atatcatgag tagtcctaaa aaattctata atgttatctg ccttgtttct agcagtagaa
     6901 gaatatagaa atctcttaaa tcccaatgca attttttctt tgtcatatat aatgtatata
     6961 gatagtataa atcccattgt catattaaat agcatagaag atatactaag tgtatagatt
     7021 aagaagtttt taggaatttt accaacaaaa ttacttgctt cttttaataa attatctaaa
     7081 ctatgttgta tatgaggaat taaagttttt agaaaatata tgtttgacat atttttttct
     7141 ataaaactat ctatataatc agtatacata ggcatttcat ttatcaatct atttaatgta
     7201 ttagctattg atggaattaa taacctaatg gcaaaggcta aaataaaacc tataaatata
     7261 taagataaaa aaatggctaa aagtctatgt gctttgaatt ttatctcaaa aagcatcaca
     7321 agtggattta aaagtaaagc aagtaaaata gctaataaaa atgggctaaa aaactttaag
     7381 aatccttcaa tactagatat aaagtctgat ggagtattaa taaatttaaa aaataaaatt
     7441 ccaataaaca cgactattaa ataatatttt atattttcct ttgaaataat aatcacttcc
     7501 ttaaaatagt gtttagtcag ctattacata ttaatagtat gtgaaaagaa attacaatta
     7561 tttaggaaaa ttaaataatt tttttgcttg tttttaatta aggtctagta tagtataata
     7621 atgtagcaaa attaacattg ataaaaatat taaacacaca taatttaaga taaggaagga
     7681 ttaagtatga gaattaataa gtatatagct tcttgtggaa tagcatcaag aagaaaagca
     7741 gaagaaataa ttcttggagg aagaataaag gtaaatggaa gtgtagtaaa agagttatct
     7801 tttaatattg atgaagaaaa agacatagta gaatttgata ataaaaaagt aaaaccaagt
     7861 gaaaattata tatatatagt attaaataaa ccagagggat atataactac agtaaaagac
     7921 caattcaata gacccagtgt aatagacata cttaaagatg taagggaaag agtatatcca
     7981 atagggagat tagattatga aactagtggt ttattaatac ttactaatga tggcgattta
     8041 acatataagc ttacccatcc aaagcatgaa attgataaaa tgtatgtggc aagtgtgaag
     8101 ggaattataa gtgatgatga gataagacaa ttcgaaacag gactaaaaat agaagattac
     8161 acaacggcac ctgccaaaat aagggtaact aaagaaaata aagaaaaaaa ttattcagtg
     8221 tgtgaaataa ctatacatga gggtagaaat agacaagtaa gaaaaatgtg caaagctata
     8281 aatcatccag ttttaaattt aagaaggatt tctgtgggga aaatagtatt aaaagacacc
     8341 aaagttggag aatatagata cctaacagaa gatgaaataa aatatttaaa atcaataaaa
     8401 taagataaaa ataaattgag aaaaaatgaa ataaagttaa tagtagggag aaatagtatt
     8461 atggcatatg caaagtattt aactaaaatt acagatataa ataaatttta tttagaaaat
     8521 attataaatg aaggtgacat agtaattgat gctactatgg gaaatgggta tgatacaagg
     8581 tatctagctg agaaggttgg agaaaagggc tttgtatatg cttttgatat acaagaagaa
     8641 gcaataaagt ctacaagaaa gaaactagag aaagaaggat atacagatag agtaaaattg
     8701 atatttgatg ggcatgaaaa aatgaatcaa tatataaaag aagaagtttc atgtgtttta
     8761 tttaatttgg gttatcttcc aagagcgaaa cattcaatta taaccaaacc agatacaact
     8821 ttaaaggcta taaagttaag tttagaaatg ttaaaagaaa atggagtaat aagcattgcc
     8881 atatatacag ggcatgatgg aggtcaagaa gaaaaagatt gcatatataa ccttgtcaat
     8941 aatttaaatc aagatgaatt taatgtttta gaatctaagt ttttaaatca agtcaataac
     9001 ccaccacaat tgattttaat tgaaaagaaa aaagcataat aaatactaaa aaacttgaca
     9061 agattgtatt aagagttaat atatatagtg tgacctatta aaaatacttt aaataataag
     9121 tatacaacag tttattctca aaacaaaata tacatctaaa tatgcaaaat aagatagata
     9181 acttttaaat gagtaaaaat tgaaaaattt aaaagaaata ttggataaat attaaaaaat
     9241 gcaaggaaag atggtataat aaatttaatc gaaaatacta tgtgaagtat aaggtacaga
     9301 aaagttagaa ttttatgagt ttaagaagct atgataaaaa ctaagatgaa attgagataa
     9361 aaaataaaat ttttagagaa aaagacagtg ttaataaaac aaaatagttt atataactaa
     9421 aaggagaaaa aaatggaaga aacaaatgaa atgaaggaca gtaaacattt gatatcaaat
     9481 atacaatctc aatatacgag attgagtaag ggtcaaaaac ttatagcaca atacatttta
     9541 aataattatg ataaagttgc atttatgact gcatgtaaat tgggtgaaac cgtaggagta
     9601 agtgagtcta cagttgtaag atttgcaaat gcattaggtt atagtggata tcctaaatta
     9661 caagctgcac ttcaagaact cataaaaaat aaactaacta cagtacaaag agttgaaatg
     9721 gcacatgatt attccgatga ttttgcaata ctaaacaaag ttttaaaaag tgatatagat
     9781 aatataaggt ctacactaga agaaattgac gagagagcgt ttaaagaagc ttctaacaaa
     9841 cttttaagag cgagaaaaat atatatactt ggaatgagga gttcttttgt agtagctcaa
     9901 tatttaggtt tttacttaga tataatactt gacaatgttc atataattag aatggatatg
     9961 ggagatgctt ttgagcagat tgtaagaata aatgaagaag atgtaatagt tgctattagt
    10021 ttccctcgtt attctaagaa atcttatcaa attgtcaatt atgctaaaga aaaaggagcc
    10081 catgtaatat cattaacaga tagcttattt gcgccagtag catcacttgc agataacact
    10141 cttctagtta agagtaatat ggcttcattt gtagattctc tagttccagc tcttagtatt
    10201 tcaaatgccc ttgctatatc tgttgggatg aaggaaaaag aagatattaa acagcatttt
    10261 gatgatttag agcaaatttg gaaaagatat tctgtgtatg agtaaagttt gtatattaat
    10321 aaggctatga aattgagaat catagcctta ttagtaaata ctcgattatc acttaaaaaa
    10381 taaattttcg tttaaataat acaaataaat atttcgagac tcaaaacata aagatgtatt
    10441 ctgaatacat ctttactacg tcaactaatt tattaaagaa agcatttcca ttcatggaga
    10501 gtgctttttt tatttctcta aacaatatca tttccttaat taac
//
LOCUS       DAYASV010000130        10500 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYASV010000130 DAYASV010000000
VERSION     DAYASV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10500
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.133"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..998
                     /locus_tag="ACGI68_003082"
     CDS             <1..998
                     /locus_tag="ACGI68_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0139503.1"
                     /translation="NKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEK
                     YKYILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDK
                     LRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEK
                     LLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLT
                     NINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSMHGKATEIRIFITFNTSNLIITI
                     SDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            991..1638
                     /locus_tag="ACGI68_003083"
     CDS             991..1638
                     /locus_tag="ACGI68_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0139504.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTIKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1767..2702
                     /locus_tag="ACGI68_003084"
     CDS             1767..2702
                     /locus_tag="ACGI68_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0139505.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGN
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2716..3813
                     /locus_tag="ACGI68_003085"
     CDS             2716..3813
                     /locus_tag="ACGI68_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139506.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3816..4880
                     /locus_tag="ACGI68_003086"
     CDS             3816..4880
                     /locus_tag="ACGI68_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139507.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5124..5621
                     /locus_tag="ACGI68_003087"
     CDS             5124..5621
                     /locus_tag="ACGI68_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFH0139508.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5797..6045)
                     /locus_tag="ACGI68_003088"
     CDS             complement(5797..6045)
                     /locus_tag="ACGI68_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139509.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6111..7127)
                     /locus_tag="ACGI68_003089"
     CDS             complement(6111..7127)
                     /locus_tag="ACGI68_003089"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFH0139510.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCVNSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7185..7952)
                     /locus_tag="ACGI68_003090"
     CDS             complement(7185..7952)
                     /locus_tag="ACGI68_003090"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFH0139511.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(8020..10209)
                     /locus_tag="ACGI68_003091"
     CDS             complement(8020..10209)
                     /locus_tag="ACGI68_003091"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HFH0139512.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYGISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKKFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEGDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3660 a         1605 c         1200 g         4035 t
ORIGIN      
        1 agaataaatt tatacttatt tcttactttt tagaacttat attaattttt atactttgta
       61 attatacaaa aacttttaat tcgatttact ttgttccttt aatattagat atatcatttt
      121 taataaagga aaaatataag tacatattgt tctcttttgt tataatatct tctcttatta
      181 ttagtttaga taaaaatata tatttggcac tggagtcaag ctctatattg ctaattataa
      241 ctgtactttc tatatatata gaaaatgaga atttatcaaa attatacagt caaaatcttt
      301 atgataagct tagaatttct aaagatgaat taaaaaaagt taatgctgat ttggaaactt
      361 atgcaagttc tatagaagaa ttagcaattt taaaagaaag aaatcgaata tcaagagaaa
      421 tacatgatag tgtaggacat tctctttcta caacaataat acaattaaat gctatagaaa
      481 aactcttaaa agacaaacct ctaatttatg acttagtaca tgaacttaga gaatttgtaa
      541 ctgaaagttt tcaagatgta agaagggcta tatctgagtt aaaacctgtt gaatatgaaa
      601 attaccaaag tttatttaaa atcaaagaat tagttaaaag ctttattaag cttacaaata
      661 ttaatgttaa attaactatt tcaaaaaata cttggaactt atccagaaat caatctatag
      721 ctttatatag acttatacag gaatcccttt ccaactcatc gatgcatgga aaggcaactg
      781 aaatcagaat ttttattaca ttcaatactt caaatcttat aattactatt agtgataatg
      841 gtataggatg tggcaatata aaaaaaggaa atggacttaa tagtatcaat gagagaatat
      901 atgaattaaa tggtaaagtt gaatttgata attctaataa tggtttcaca attagagctt
      961 cttttcctaa attttcaggg ggtgattttt ttgaataaaa ttaaagttct tatagtagat
     1021 gacgaaaaat taattagaaa aggtcttaaa attatattat catcttataa tgatttagaa
     1081 attgtaggag atgcttctaa tggatatgaa gctttagaat tttgtaaaat gaatgatgta
     1141 gacatagttc ttatggatat acgaatgaaa gtatgtgatg gggttctagg tacaaggctt
     1201 ataaaggaat ataataattc tataacatta ttaattttaa caacttttaa tgatgatgaa
     1261 tatataaaag atgctatgaa gtttggtgct tcaggatacc ttttaaaaga tagctctgat
     1321 gaagtattgc atgaagggat tcgttcttca ttttttggca atattgtgct tgataaaagt
     1381 gtagctgaaa aaatcatgac ctctgaaaaa acgattaaac aagagcatct ttatgacatg
     1441 tacaatttaa cagaaaaaga aatatctata attagactaa ttgcaaatgg acttaataat
     1501 aaagaaataa gtcaagaact attcttatct gaaggtacaa taaagaataa cataactaat
     1561 attttagcta aactagaact tcgtgacaga actcaactag caatctttgc tttcaaaaat
     1621 aaaattgtaa tagaataaat ctaaaaatat aaagtgactt aagtcacttt ttttagtgac
     1681 ttatagtaat agaattataa ttcacaattc tttattataa tattatagct ttttaactta
     1741 tacaaaatta ttttggaggg attactatgg atatagtcag agtaaataat ataacaaaaa
     1801 gatttaatga taaattagta cttgataaca tcagcttttc tgttaaaaaa ggtgagatat
     1861 ttggacttat tggcccaaat ggtgcaggta aatctacact tataaatata attacaaatc
     1921 ttatgttacc aaatagtggt agtattcaaa tcaatggttt agatttatca aaagattata
     1981 taaaagcaaa gtctataata gggttagttc cacaagaatt agcaattata gaaactctta
     2041 ccccttttga taacttagag tactttggag ctttctacgg tttaaaggga aaactactta
     2101 aagaaagaat cattgaagca ttagaagtta caggtcttac tgaagtcaaa aagaaaaagg
     2161 tcaaaaaatt atctggtggt atgcaaagga gattaaatat tggaatagca ctcttaaatc
     2221 atcctaaaat attaatattg gatgaaccaa ctgtaggtgt agacccccaa tcaagaaatc
     2281 atattttcaa ttttataaaa gatatatcta aaaaacatga aactactgta atatatactt
     2341 cccattatat ggaagaagtt gagcaccttt gttctaaaat ttttattatg gatgaaggta
     2401 aagaaattgc ttttggaaat aatgattatt taaaatcact cgtatctaca aatacaaaat
     2461 taattatgga aataaaaaat ataaatgccc aacttatctt tgatttaaaa aatactaaag
     2521 gtgttatatc tgttttagaa aataattcat tattagaatt ggatatagat aaaaaattac
     2581 aacttacaga tatactctct gtcattgaca aaaacaattc aaaaataatg aaaatttctt
     2641 atgaagaacc tagcctagaa gacgtatttt taaatctaac aggaaaaaat ttaagggact
     2701 aatggaggtg attttatgaa attatacatg agtgtaaaaa ctatgctaaa gagtttaaaa
     2761 agctccttta tacttaattt aatatatttt ttagctttgc ctctgatttt atcttggttt
     2821 cttgggatgg ttacagaaag catgtttcaa aaccctataa aaacagaatc tacccccatt
     2881 gttatatatg acaaagataa cacaagatta tcaaatgatt tgacaaagta tttgaaaaat
     2941 gatttatctt atatattaac agttaaaaaa gatgattcta aggcagaatt aaagcttact
     3001 atacctaaag gctatgagag tagtttatta aatgaaaaat caaatactct aaacatagaa
     3061 aaacttggaa ctcgtgatga tattgcaata ttgcttcaag atatattaga tacttaccat
     3121 gaaaaatttt atttaaataa ttctcagaaa ataagttcag aagatttttc taaattattt
     3181 aataaaaatt ctattgatac ttctataata aataacaatg ttaaacagag ctcttatgaa
     3241 tactttgctt tagtttcact aggatttctt gtaataattt ttataatgaa caatattttg
     3301 tcaaattata taagtgaatc aaagggactt tcaaaaagac tttattctat gcctataact
     3361 agagtacaat tcttaattta tgactttgta ggtttatgga tttattcatt tatattctta
     3421 ttattatatg ttttattctt tagaatactt ggaataacat ttaaaggtaa ttttgccata
     3481 ttgctcctac tttgtgcttt atcatcttat tttatgactt caatatctac ctttgtaacc
     3541 agctttttta gtaaaaaata cggtacagca atagtttatg cattactctt tttacagaca
     3601 atttttggag gtatcttcag tatgataagt gatacattta ctaagcttac aagtttatct
     3661 cctacctatt taataggaga attgtttagt aattatgaaa catttaaaac catagattca
     3721 ataggaaatt taattttcac atgtttaatt atctctacaa tattaattgc acttgctatt
     3781 gtaaaggaaa aatataaatg gagggaggtt taataatgat tagattaact attgcaaata
     3841 taaaaagata tctaaaaaat catactcttt taattaatat ggttatgctt cctattatac
     3901 taattttttc acttaatttt tttatcaaca actcgggaaa tcaatcaatg tacttttctc
     3961 cagtagcaat tgtatctgac tcttcaggaa agtatgaaca taagttaata aatagttcta
     4021 aattaaaaga aaattcattt agattaaatg aacaagataa ggctatgaat ctattaaaaa
     4081 ataataaggt ttctgctgta ttcgttttag ataaaaattt ttcaaatagt atagataaat
     4141 tacaacgacc tgtagttaaa tgctttaaaa ttgaaaatgg tggtggtagt ctttgggcag
     4201 aatcacaaat agaatctttt attactaaat cgttaaaatt gaaagtggat aaaaatatag
     4261 atgataaatt gacaaagaca aatataattg ataacaaacc agataagaat aaaggttcat
     4321 ttttagtagt cttcttgata tgctatttta tgtatattaa tgcggcacat cttgcttctg
     4381 atttatttac attaaaaaaa tcaaatgtgc taaaaagact tatctctaca gataataaag
     4441 atataaaaat aattttcagt atatttttag gactattttt tattcaatca attgtataca
     4501 cttttgctct attatgtttt tcaattatag aagactttaa tttgagtctg aatgtattga
     4561 tgattgtact agcaaatagt tttgtttcca ctggatttgt attttgggta gctagagtat
     4621 ttaaaaatga aagttctata agtcttatat ctacatttta ttctttaata ggtctagcta
     4681 tatctatctc tagtctaata ccatcaatgg ataaattatc ctttatgact aatctttcaa
     4741 agttcacacc attttattgg actatagatg ctattaaaaa caatggtagt attttaataa
     4801 atataatagc tctaatatta attggaatta tatttgtaac tgcaggaagt cttaaactaa
     4861 gagattttgc taaaaactaa taattattaa atataattag tatctatttt tatataattg
     4921 taaaagaata tttcaacata ttaaattatt ttatcttata tttttctctt tagtttcaca
     4981 catcaccaag tctaaactct atattatata ttaatatttt tgacttgtca agttatttat
     5041 gttataatat taatcattag ataattaaag cattgtacct aagttgcaat atctaaaatt
     5101 caatttggag gtataactag catatgtata aaattggtga aagtgtaatg tatccaaaag
     5161 agggagcctg ctctgtaaat gatattgtaa caaagaaaat aaatcatgag atgcaaaaat
     5221 actatgaatt aagtgtcatt tttaatagta accttaaaat ttcgattcca gttctaaatg
     5281 cagatagaat tggcatacgt ccagttatgg atggaaatga tgttgataat tttatccaat
     5341 ctattaataa aacagatggt gtatggattt ttgatagaaa agaaagactt aaactgtacc
     5401 aagataaatt tcattcagga gatgtttttg aaattgtaaa attaatcaaa atgcttatga
     5461 tacaagacag ttcaaaacaa ttgtgtagca cagataagga gtttttaaat aaagctcaaa
     5521 aatttgcttt atcagaatta gctgctgctc aatgtaaatc ctatacaatg gtattagagg
     5581 aaatgaaaaa acatatatta aattcaaaga atacaaatta aaccagtttt ttaaactggt
     5641 tttatttttt atactaatca tccatgctac tttagcttat tttaaactgc ctaatctaat
     5701 attaataaag ttatctcaat tagatagatg tattttattc aaataaaaaa aactaagagt
     5761 aaatcatatt actgatttac tcttagctaa taaaatttaa tcttcttcaa gttctttttt
     5821 ctcttgtaat aatacactta attcatttgc ttctaaaact tgtgcctctg caatatcaag
     5881 taactcttgt tgccattcca cacatttatc tttgtaatca aaaggtagcg ttctgtcaac
     5941 tatgtactta tcaatacagt cctccgcact tttaattatt tcttttcttt catcaataaa
     6001 tttttctaat tctactaagg cttcttcata agcttttcta ctcatataaa ttcccccttt
     6061 gtacaattat tctacattaa aatgattttt cctctttttt gtataaaatt ttatttgtat
     6121 ataacttttc catcaatatc ttttatagtt tgacatccag ttaatatcat agaactactt
     6181 agctctgatt gaagtttatt tacataagtt tctacaccat cagttgcacc accaaaagaa
     6241 gcagtcacaa aaggtcttcc tattaatact gcatctgcac ctaaacctat catttttagt
     6301 acgtctaaac cagttctcac tccaccatct gcaagtattg taactttacc tttaacagca
     6361 tcagcaattt ttggtaaaac ttcacatgct cctggtgtac agtcaagcac tcttcctcca
     6421 tgatttgata cgactattgc atccacacct gcttctactg ccattaaagc atcttcaaca
     6481 gtcattatac ctttcaatat aaatggtagt ttagtagact ttactaactc ctttatttgt
     6541 ttaacatttt taggaagtac tggttttcca tgtaatgata aagttactag accacaggca
     6601 tctatatcaa ctccaactgc aaatgcacca gcttcttcag ctaatcttat cttttttata
     6661 atattttcat tatcccaagg tttaataaat acaattccag ctccattata ctttttgact
     6721 acatctaaat tttccattaa aaaagcatct actgccgtat caccaaccat tgcataaata
     6781 ccactattaa cacatcctgc aactactggt tctatgtaat ctctttcatt aatttttcca
     6841 cccatattta aagtagttcc tgttactgga gctgcaaata taggtgcact cattttcttt
     6901 ccaaacatct ctatagatgt atcaggattt gatacatcat gtattactct catatttact
     6961 tttacttttt ctaaactctt gctattttct ataaaagaag aaccacttcc tttacctccc
     7021 atgccaggca cttcacctgc acaagcaacc ccattacaaa ccttacaaac tttacaactt
     7081 ccatttaaat tttctctagc actttttaat aattcattat attccatctt taatccccca
     7141 ttaactatag taatttatat tatatacatt atatcatatt atatttacag tatatttttc
     7201 aaaaaacttt ttctgtggat aggagttatg ccatattttt caatagcttc atagtgttct
     7261 ttagttccat atcctttgtg agatttaaac ccatattctg ggtatactct atcatattgg
     7321 tacattatac tatccctagt aactttagca agtatacttg ctgctgctat tgatatagat
     7381 tttgaatctc ctttcacaat tggattttga gatatgtcaa tacctggtat tgttgctgca
     7441 tcaactaata aataatccgg tgcttttttt aaacaattta tagccttttt cattgccata
     7501 tatgtagcat ttaatatatt aaattcatct atttcttcat tgtttactat acctattcca
     7561 taatctagtg cttcttcttt tattacttca aaaagttcat ctctttttgc ctcactcagt
     7621 ttcttagagt catttacacc ttctattttg gtatcttttt taaatactac aacagaagct
     7681 acaacaggtc cagcaagtgg tcctcttccc gcctcatcaa ttcctccaat atataaataa
     7741 cctttatcat agccttcgtt ctcaaatata tttatagttt ccaatctttc ttcttctttt
     7801 ctaatattat ccagcttttt tgccagtttt atagctagac cttgtacaga ttttctttca
     7861 tcaactctta acagctctat atattccata tatttttcta cttctaaagt ctctattatt
     7921 tctttaatct ccctcacact tttatcctgc ataccattcc ctcatctttc gtttttaaaa
     7981 atttaatttt taaatatatt aaaatttttt aattagagtt taattgcatc cactatagcc
     8041 acattttaaa catatactac atccacctgt atgtgcaatt ccttcacttc cacactcagg
     8101 gcacacaaat ttttcatctc cttcaaaatt tttatttatt atttctttgg ctttttcatc
     8161 tttattatct ttttcttttt tattattttt ataatattct tttttactat tttctttttt
     8221 atcttcttta ttattttctt cctttttttc atttattaaa attccactcc ttttacatcc
     8281 atctctaaat attgttactc ctttaagacc agcctcccat gccttaatat atatattgaa
     8341 aacatcttca actgtagttt caaaaggtat atttattgta gatgatatag aagcatcaat
     8401 atacttttgc catacttttt gcatgtttat tctttcttca tatcctatat tcatagcagt
     8461 aacaaaaaaa tctggtaaac attcttcatc tgtaatacct ttcttgtcca tatactcttt
     8521 aacaattgga gtatatactt tataatacac atcctcatca tgcaagcttt cagtttttct
     8581 tatatatgaa agattaaata taggttctat tccaccactg atacctatca ttgtagatat
     8641 ggaacctgta ggaggtatgg ttaaaagttg tgaatttcta agcccatact ttttaaccat
     8701 ttccttagtg tcttcattta gattttctat aaaaaactta gatttagaaa tattttcata
     8761 tttatactca ctaaatgttc catattcttt agcaattaat gctgattgct tcacagcttc
     8821 atttaacata gtcttaccaa ttaaatcaca tagttctaaa gattcatcag aaccatatct
     8881 tattcccatt tttataagca tatcagctat acccattaca cctaaaccta tctgtctata
     8941 cttatgtaca ctatctcttt gttcttgtaa tggatgcaag ttcaatccct catctaagac
     9001 atcatttaaa gctactacag cttctctaac gcaagatttt aatttattaa tatcaaaagt
     9061 agcatgttca ccaaatggtt gtattataaa ctctgacaag tttattgagc caagtagaca
     9121 acttcctcca cttggaagtg gttcttctgc acaaggattt actcctgaaa agcaaaagtt
     9181 cttatcttca ctcataaaat tatatgtttt tattttatcc caaaacaata tacctggttc
     9241 tgcataatcc cagttatttt tcacaaattt attaaataaa tcataagcat ttactatctt
     9301 aactattttt tctccagtag tttcaacttc aaaagtacaa ttatataact ttttttctct
     9361 tacagcttgc ataaattcat ctgttactct tacagaaata tttgctttag taaccttctc
     9421 caaatctgtt tttatatcta taaagtcttc tatctctgga tgatttacat ccatagaaat
     9481 cataagagct cctcttctcc ctctttgacc aattaagcca gttgtcatac tgtaaagttc
     9541 cataaaagag actgctccag tcgtatgttt tgcagcattg ttaactttcg ctccttttgg
     9601 tcttagcttt gaaatatcaa ttccaactcc tccaccataa ctaaaagttc tagcaagttt
     9661 ttttgcagta tcaaatatat cttctatgct atcttttggt ggttctatta cataacaatt
     9721 agaatatgtt actttttttc cctctttctg taatcctcta tttgctaaaa ttcttccagc
     9781 aaatagaaat tttttttgtc ttattagttt tgatattctt gaactattat ttgaaattct
     9841 ctttatccat tcatcaaaac tctcaccatt atgtctatac ttgttcttcc atattgattt
     9901 ttgtaattca tcaaactccc aagctttagt ccttatttca gagcgtcttt ctctatataa
     9961 tatgtagtgt tttgctatat cttttctatt tgattccata agtagtactt caactatatc
    10021 ttgtatttct tctacggata agcatttttc catagaatta tagtcattag ctatttttct
    10081 tgatatacca tatgccaact ctctatctac tccaccttca ctatttatac tagctttata
    10141 gacagcatct tcaatcttac ttttactaaa aagttctttt gtaccatctc tttttattat
    10201 gtatttcatt tttaaacctc caataaatta gtattaaatt aatacacaca ttaactataa
    10261 ctctaaacaa actttttata tatttgtata ttttcatatt aattatctaa cataatatat
    10321 attatactat tttatattca atataacact ccttatagtt ttcttaactt gtaaaatatc
    10381 ttcctaaaaa actcaaaaat aatataaaaa agtatataaa agtcaaatta tactcttata
    10441 tactttatct ttttatattt ttttcatttt aactttgatt aattaataag tatttagaaa
//
LOCUS       DAYASV010000131        10426 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYASV010000131 DAYASV010000000
VERSION     DAYASV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.134"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            166..2667
                     /locus_tag="ACGI68_003092"
     CDS             166..2667
                     /locus_tag="ACGI68_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFH0139513.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVKNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2827..3495
                     /locus_tag="ACGI68_003093"
     CDS             2827..3495
                     /locus_tag="ACGI68_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139514.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3479..4756
                     /locus_tag="ACGI68_003094"
     CDS             3479..4756
                     /locus_tag="ACGI68_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139515.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            5157..5378
                     /locus_tag="ACGI68_003095"
     CDS             5157..5378
                     /locus_tag="ACGI68_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139516.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5667..6431)
                     /locus_tag="ACGI68_003096"
     CDS             complement(5667..6431)
                     /locus_tag="ACGI68_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0139517.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6705..7379
                     /gene="fsa"
                     /locus_tag="ACGI68_003097"
     CDS             6705..7379
                     /gene="fsa"
                     /locus_tag="ACGI68_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFH0139518.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVGKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7501..8895)
                     /locus_tag="ACGI68_003098"
     CDS             complement(7501..8895)
                     /locus_tag="ACGI68_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFH0139519.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(8973..10319)
                     /locus_tag="ACGI68_003099"
     CDS             complement(8973..10319)
                     /locus_tag="ACGI68_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0139520.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
BASE COUNT         4241 a         1236 c         1610 g         3339 t
ORIGIN      
        1 aatataacta atacatttat ttattaaaaa aatattgatt taatatacct atagggggta
       61 taataaaaat atagatttct aaataagtaa tttatcaata ttacatatca aaataaaacg
      121 ttacaaatta gctgaaaata tattgaaaga aaggaagatt ttattatgag ttcaaataaa
      181 gtggtagaaa gttataaaat tacaggtatg acatgtgcag cttgtgcaaa ggcagtagag
      241 agagtaacta agaagatgga tggagtatat gaccaaagtg taaatatagc tactgaaaaa
      301 cttaaaatag aatatgataa ttctaaggtt aattttgatg atataaagca ggttgtagaa
      361 aaagcaggat atggaattat aaaggaagaa agtaataaaa aaattgatat gaaaattgat
      421 ggaatgacat gtgcagcttg tgcaaaagct gtagagagag tggtaaaaaa attggatgga
      481 gtagaaagta ttagtgtgaa tattgcaaca gataaagcta atatagacta tgacccatca
      541 aaagtaaaat tatctcaaat aaaagcagct attgaaaaag ctggatataa accaatagaa
      601 gaggtaaaaa ataaggttga tgtggatgaa gataaattga gaaaagaaag agaaatgaag
      661 tctttatttg ttaaatttat agttgcaata gtatttgcag tacctctgtt ttatatagca
      721 atgggaccta tgataataaa gcctataggg ccatggcctt tgccagaaat tataaatcct
      781 atgacaaata cttttaatta tgcattaatt cagttaattt tagtaatacc agtaatgata
      841 gctggttata aattttatat aaatgggttt aaatcacttt tttctttaag ccctaatatg
      901 gattctttag ttgcaatagg aacattagcc gcatttttat atagtctata tacgacacta
      961 caaatagcca atggacaaat tcaaggtatg caccatcatc agttgtatta tgaaagtgca
     1021 ggaataataa tagcacttat cttacttggt aaatatttag aatcaaaatc aaaaggaaaa
     1081 acatcagaag caattaaaaa gcttatgggg ctacaaccta aaactgcaat agtgttagtt
     1141 gatggtaaag aagttgaaac tcctatagaa gaagtagaga taggagatat actattagta
     1201 aaaccaggaa ctaaaatacc tgtggatgga gttgtaatag aaggatatac atcagttgat
     1261 gaatcaatgc ttacaggtga aagtatacca gtagaaaaga atgttggaag taaggtgaca
     1321 ggagctagta ttaataaaaa tggtgttatt aagtttaagg ctgaaaaaat tggtggagat
     1381 actgctcttg cccaaataat aaagttagtt gaagatgcac aaggtacaaa agcacctatt
     1441 gctaaacttg ccgatactgt atctggatat tttgtaccaa tagtaattgc aatagctgta
     1501 gttgcatcat tattgtggtt tttaatagga ggaaaagata tagtatttgt acttactata
     1561 tttatatcag tacttgtaat tgcttgtcct tgtgcattag gattagcaac accaactgct
     1621 ataatggttg ggactggaaa gggcgcagaa aatggaatct tgataaaagg tggagaggca
     1681 ttagaatctg cacataaagt aaatacagtg atatttgata aaacaggtac tataacagaa
     1741 ggaaagccaa aagtaacaga tatagtatta aataataatg taaaagaaga atatcttatc
     1801 aagattgctt caagtgcaga aaaaggttca gaacatcctc ttggagaagc aatagttaag
     1861 tatggagaag agaagaatat aaaatttgaa aaagttgata attttaaagc cataccaggt
     1921 gctggtatac aagttactat taatgatgaa agtatattac ttggaaatag aaagcttatg
     1981 aatgacaata atataaagct tggggattta gaagaaaaat caaatatatt ggcaagccaa
     2041 ggtaaaacac ctatgtatat agctgtagat ggaaatttat caggtataat agcagttgca
     2101 gatgtagtca aagaaagtag taaaaaagca atagaaatac ttcatgatat gggaataaaa
     2161 gttgcaatgg taactggaga taatgcgaaa actgcaaatg ctatagcaaa tcaagtcggt
     2221 atagatatgg ttttggcaga ggttttacca gaagataagt caaaagaagt tgaaaaacta
     2281 caaaatcaag gaaagtttgt tgcaatggta ggggatggaa taaatgatgc cccagctctt
     2341 gcaaaagcag atatagggat agcaatagga agtggaacgg atgtagccat tgaatctgcg
     2401 gatatagtac ttatgaagag tgatttaatt gatgttccaa cagcaattaa acttagtcat
     2461 gaaactataa aaaatataaa acaaaaccta ttttgggcat ttggatataa tacaattggt
     2521 atacctgtag cagcaggaat attatatgta tttggaggac ctttattaaa cccaatgatt
     2581 gcagcagcag caatgagctt aagttcagtt tcagtcgtat caaatgcact tagacttaaa
     2641 aacttcaagg catataagag agattagatt aataaattaa ataaaatgta tccaaaagag
     2701 ctatctttaa attaaaagga ttaatttaca aattaagata gctctttttt catgtaaaat
     2761 tcacaacaat aagataaaat ataagtaata tacgaaattt taaataaaat agaaaggatt
     2821 gatagaatga attcgtcaat acttgtaatt gaagatgatt caaatataca agagttaata
     2881 tcagaatttt taagtgcaga aggttatcaa gttgatacag caaatgatgg gttggaaggt
     2941 atacaaaagt ttaagcaagg aagttatgat ttagtaatat tagatataat gatgccaaac
     3001 ttagatggtt atggagtttg taaaatgata agaaagtcat caagcgttcc aattatattt
     3061 ttaactgctt taaatgatga aggagaccaa cttaaagggt ttgacttaga atgtgatgat
     3121 tatataacaa aaccattttc atttaatctt cttataaaaa gagtagaagc aattttaagg
     3181 cgaagtaaca agactataaa tgacaaattt atagtttttg aaaaattaaa attagattta
     3241 aacacatata ttgcagagat agatggagaa cctatagaac ttacactaaa agagtttaat
     3301 atattaaaag ctttgataga aaagtatcca caagttataa ctagggaagg tcttttggat
     3361 agtatatggg gttatgacta ttatggagat actaggattg ttgatgcaca tataaaaaat
     3421 ataagaaaaa aaatatcatt accatatatt aaaactgtaa aaggaatagg atatacactt
     3481 gagaaggata tttgataagt gggaaaagtt aagtataaag tataagttat tttcaataac
     3541 gacatcatta ttaatagcat tagctttaat aatatatcta attttatatt ttttattgcc
     3601 atcatattat catgagtata aaattgagtc tcttcaagaa agtttgaaat ctttagtaga
     3661 tagttcaatt catttcgata catatacttt agaagaaaga ctttattata tggctaaaga
     3721 ccaaaatttg gctattttat taaaagataa ccaaggtaaa attgtatatg gtaagaatga
     3781 agtagttatt ttaagatata gtaagtacat gattaatagt cttgaggatg agtatagaac
     3841 ttctatacct atatatacta aagatgcaaa agatggtcca tatactttag aactcgttat
     3901 gccattacaa ccaattgacg aagcaaacga agttataaga aaactaatgc catacatcat
     3961 atcaatagca atattgatag cgattattgg agcatatata tattcaattg taataacaaa
     4021 gcctcttata aacataatag agagtgagag agaacaggaa tatagaagga aggattttgt
     4081 tgctacaata tcacatgaac taaaaactcc aataaccata ataagtggtc aaattgaagg
     4141 tatgatttat agtgtaggta agtataagga tagggatact tatctgaaaa aatcgtatga
     4201 gtgtacacaa gaattaaaag atttagtaaa tgaaatgata gaggtttcaa aatcagaaat
     4261 acttgaaaaa gatttgaaat tggtatcaat aaatataagt gaattattaa atagacttgt
     4321 aaagagacaa gtatttttaa tagaagaaaa acatatgaaa actattttaa aaatagaaga
     4381 aaatttggaa attaaagcag accaagagag aataactaag gcaatcaata atataataaa
     4441 taatgccata aaatattctc ctgaagaatc agagcttata attagactct atgataaaaa
     4501 taaacgaata agtaaaaaga cttctaatca aagagtggtt ttagaaattg aaaatactgg
     4561 tgtaactatc gagaaacgtt atttagaaga aatatttaat ccattttata gaatagaaaa
     4621 atctcgttcg agaaaaactg gaggaagtgg acttggactt tatatagtca gccaaatttt
     4681 taaaagtcat gggtttgatt atagcataaa aaacaaagaa aactctgtag tattcacagt
     4741 agaatttaaa aattaattac ttattataaa gaatatataa ctttcaataa tagttcattt
     4801 taaaatcaca aaaaattcat aatgaaactg tattatataa atataaataa gcttaaaacc
     4861 ataaaataca ccaactataa tgatgagatt tccttacatc ctccatataa gtcatcatta
     4921 tagtaaccca acaattaagt tactatgaat ttaaaaaata acaccgagca tataatctat
     4981 gcttagtgtt atttttattt tgaaataaaa aatatttaat ttattgaaat gtatagattt
     5041 agatagttta aactatattt aagacttaaa aattataatt gatattacaa ataaatatat
     5101 gtactttggg aaaaataaat ataggaagta ttatatttac agaggaggta gttttgatgg
     5161 atagattaaa aagagatatg aaaaatatga aagaagattt aaaagaaggt gttaaaaaga
     5221 cagcttatgt tatgaaaaat acagctgaag atttgaaaga tgatgcacaa ggtgctatgg
     5281 taaacttaga aatgaaaaaa gatgagatga tggaaagata tgaacaaaag aaattttcta
     5341 aagaattaga gaagcaaatg aagaatgaaa tgaaataagc ttttatttat gaataacaaa
     5401 ttattgtatt catataaaaa gtagccagta atattaaata gtttatttaa ggaaatagac
     5461 tttctaaaaa taactgtatt agatttagaa agtctatttc aaattagttt tataaataga
     5521 cataaggata aagtagatgt tatagaacta gattttaaaa aatagatact aaggataaaa
     5581 taaatattat agaaatatgt ttttaagaat atttatcaaa taaaaattta tatttataag
     5641 aaatcctata aaacatataa ttaaacttat aaaacattta cattaaattc tgataaaata
     5701 tctactatat caggagtagg cgttttttct gtaattatag aactaatatc ttctaaatga
     5761 gcaaacttga aatttccatc aaatttaaac tttttatctt ccataactat aaatactttt
     5821 ttacttgaat ttattatagc cttcttcgta agaccatctt caatttcaaa agttgtaagt
     5881 gaagagttga atacatctac tccacaactt cccataaaag tcttatcaaa cttatatttc
     5941 gatataaact ctatagttgg agtaccgaca aaaccgtcta aactgagatt taataaacct
     6001 ccagtggaaa ttatatttaa attatttggt tttgaagtaa ctacatttag tatatccagc
     6061 atatttgtaa caagtgttac ttttttatta ctttcagcta acaacttcgc aagcaagatg
     6121 ttaattgtag agatatctaa aaatatagtt tctctatctt caattaaatt aaatgctttt
     6181 tctgctatta ttttctttgt tggaatatta atttcttttc tgtcttttgt atcctgattc
     6241 tctagtgttt ctctagctag tacagctcct ccataaattc ttcttattga agaaatcttt
     6301 tctatatttt ttaaatcttt tcttatacaa tcttctgtaa cattaaattt agaactgagc
     6361 tctttgacag taactctacc ttcttttttt aggatattta atatagcttg gattctttct
     6421 tctgcaaaca ttttctacct cttttcgcaa tatagtatta tagtaaatat tataagataa
     6481 aatacaatat atcaactatt ttaacatgaa atacaaaatt aatttaatat aaaaagtatg
     6541 ttttttctaa ctatatattt agaatttagt aaataaaaaa attataaaac taagaaggaa
     6601 aagtaaaaca ataataaaaa ataaaaaaca ataagcaaaa tgttgaaaat aataccaaat
     6661 caatatataa taagaataaa gaaagattaa agggggatat tttaatgcaa ctaatcttag
     6721 atacaggtaa tgtggaggag attaaggagt tatgtacttg tttaccaata gatggtgtaa
     6781 ctacaaatcc aagtattgtt tcaaaagaaa aaaagaattt taagcaactt ataaatgaaa
     6841 ttggtgagat tataggtgag gatatgccta tacatgctca agtgttaagt acaaagtatg
     6901 aagaaatatt agaagaagca ttatatataa gtagtttgag aaaaaatata tatgtaaaaa
     6961 tacctgtaac acaagatggt ttaagagcta taaaagacct tcacagaaaa ggtgtaaaaa
     7021 taactgctac tgcaatattc actgctcatc aaggtttttt agctgcaaag gctggagcaa
     7081 attatattgc accttatgta aataggcttg ataatatatc tggtgatgga gtagctatgg
     7141 tttcagaact tataaaaata atagatactt ataaaatgga tacaaaagta cttgcagcca
     7201 gttttaaaaa tgcacaacaa gttatagaat taatgcaaca tggggtgcat agtgttacag
     7261 taccatatga tatttgtaaa tctatgatga atcatccatt aactgattgg agtgtaggta
     7321 agtttataga ggattgggag aatacttttg gaaaaggaag taaaacaaat aatatttagt
     7381 attatggtaa aagagtagtt ttgtccaatt tcacaagaac ttatataaaa agatgaaaat
     7441 atgaattttc tttttaataa tttcgttatt atcttaagtt aattcatatt ttcatctttt
     7501 ttagatttct agtcctataa atttccacca agggaatgtt atacatatta ttataaagta
     7561 ggttaaagga atcaaagtta aaccgtattt gaaaataacc ttgctattat acagattttc
     7621 accatgacca accattattg tagcatgatg aaatggtaat atgtactgta tgcttactat
     7681 aatgtagaca aatagacata aagtaacact attttccttt agtatatgta aacttccaag
     7741 taacggtatt actacagatg atgtagtgac agcacttcca agacatatat tcaagattat
     7801 tgtgagagtt atcaaaaaca atattgaaat aatttctgaa gagtttggta taaactttat
     7861 caaataattt cccattatat ctacaactcc agagtacttt aacactcctc caattgaaaa
     7921 tgctgctata aaaaaaataa ggaccttcca atttatttct tttaaagtac taaatccaac
     7981 tatttttctt agtaataaaa gaagtacaca aattgacatt ataattaatg tgtttatgtt
     8041 atgtatgaac tgagttaaaa acataaaaaa cattattcct aggcataaaa atacgtgttt
     8101 ttgctctttg tttagcaaat taaattcttt ttttaaatta gaatatgaaa aatgaagttt
     8161 attaccctca agattttcat tagaaataaa ttttattgaa tgcaattctt ttttaaagac
     8221 aaagataaat aaaaaataag ttataaaaca agtaattata gttggaacag acatataaaa
     8281 agcccattga ccccaattta tattacaatt tccaagttct agtacaacat aatttagaag
     8341 ggtgtctcca tttacaaaaa acattgatgt aaaggtagat gctgtaaata tactaaataa
     8401 aagtatagat tttgaatctt cagttatctg ttgttctttt aaaaactcct tgtaaaaagc
     8461 agatacaaga ataacccttg gaaatggttg tgggataaaa aatattaaaa gtatacctaa
     8521 aatatacgat aaaagaatca tttttttagg ggtatttaca gtatttaaaa ttaacttctc
     8581 agaaataatt tttgcgacac ctgtttttgt aactgcatta gtaattatgt atgcaagaat
     8641 tattatgtaa aaattttctg taaataaaaa ctgtaaaata acctttattg gtgcaatgtt
     8701 aaatataaac gctagaatta caaaaaacaa tgcaacaaaa gttttttcaa ctgagtctac
     8761 agcccatatc aagatagtta aaaaagataa gcatatagaa aattttatgt tgaaactgta
     8821 attaagagag aagtagagaa ttatggtagc tataacagtc atataaaatt gttttggctt
     8881 aagaataata cccattttta tattcacctc cataatttat tatatcataa tggaggtgaa
     8941 tacaatgaat gtgaatacat tttataaaaa gattattcat tgattctatg atttacaata
     9001 gctttagttt tccaatgtcc tttcttatag tagataactg taacaataag acctaaagtc
     9061 caaccaaaag gaatacccat ccaaattccg tctgttccaa aaaatttaga aagcatataa
     9121 gaagctggaa gtcttccaag ccataaagat actattgagc ttataagtgc aaacatggaa
     9181 tcaccagcac ctttcattgc acttgaaagt acaaaagaag ttccaataaa taagaagaat
     9241 ggtcctatta taaataagta agaagagcca attttaatga catctttatc agtattgaat
     9301 agagcaatta gattttccct aaatagcaaa attaaagcaa taactgtaac tgctaaaact
     9361 aaggtcattt ttatagattc tcgaatgcct tttttaactc tttcattttc tccagcacct
     9421 atgttttgag caacaaaagt agaaacagca gaaccaagat tcataatagg taagcttata
     9481 aaattttcta tcctaagacc agcaccaaaa gctgccatag cagatgtacc ataactatta
     9541 actaaaaatt gaagagccat atttccaatt gaaaacaaca tttgttgaac tccacttgga
     9601 agtcctaatc taagtccatc tttaaatatt ttgttatcat atacaattcc tttgatttta
     9661 aatttaagca cttcatgttt tttatttaaa taaattatac ttattataaa tgatactgcc
     9721 tgagccatta tagtggcaag tgcaacgccc tcaacaccca ttcttagcac tacaataaaa
     9781 gttaagtcta aaactatatt tagaattgtg gctattatca gaaaataaag tggagttact
     9841 gaatctccaa gtcctctaag tattgcactt actgaattat atccagcaga aaataatatt
     9901 ccactaaata ttatagtaag atacaatttt gattgagcaa aaacggattc tggtgtattc
     9961 ataagcttaa gcattgggcc accaaatact attccaagaa tacttattaa tatagaacca
    10021 ataaataaaa gtatatatgt agtatcaata gtagctttta ccttttttaa atctcctgag
    10081 ccataaaatt gtgacactaa aatagaaaat ccaaggctaa cacccataag cgctgccacc
    10141 aataaaaaca ttatagggtt agctccagag actgccgcca ttgcttcttt tccgataaat
    10201 ctaccaacta ttatactgtc agctgtattg tatagttgtt gaaataaact accaataagc
    10261 ataggcatgg caaaaaagaa tatggacttt ccctcatgac ctgtagttaa atctttcacc
    10321 ttattcctcc aaataaaaaa attattaatt tattatagta taatcttaac ataaacttta
    10381 tatccaaact ataaataaat tgtatcaaaa tatcatatat tttttt
//
LOCUS       DAYASV010000132        10370 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYASV010000132 DAYASV010000000
VERSION     DAYASV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.135"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..339
                     /locus_tag="ACGI68_003100"
     CDS             <1..339
                     /locus_tag="ACGI68_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0139521.1"
                     /translation="FYKKFGFSSKEMSKNYKLNARNVKINLNIKGYKFLPYVKNSKAS
                     RDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFGNMKSIYLKKTNGNLIELSNH
                     RITESKPWKI"
     gene            327..1265
                     /locus_tag="ACGI68_003101"
     CDS             327..1265
                     /locus_tag="ACGI68_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFH0139522.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1649..1873)
                     /gene="secG"
                     /locus_tag="ACGI68_003102"
     CDS             complement(1649..1873)
                     /gene="secG"
                     /locus_tag="ACGI68_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFH0139523.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2466..3758)
                     /gene="eno"
                     /locus_tag="ACGI68_003103"
     CDS             complement(2466..3758)
                     /gene="eno"
                     /locus_tag="ACGI68_003103"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFH0139524.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(4346..5875)
                     /gene="gpmI"
                     /locus_tag="ACGI68_003104"
     CDS             complement(4346..5875)
                     /gene="gpmI"
                     /locus_tag="ACGI68_003104"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFH0139525.1"
                     /translation="MKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTLI
                     NASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMDN
                     AKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALEY
                     AKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAIQ
                     NSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDGF
                     KREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAHV
                     TFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVLN
                     FANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPET
                     GKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTILDMMKLEKPEEMTGHSLIS
                     K"
     gene            complement(5887..6630)
                     /gene="tpiA"
                     /locus_tag="ACGI68_003105"
     CDS             complement(5887..6630)
                     /gene="tpiA"
                     /locus_tag="ACGI68_003105"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFH0139526.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            complement(6656..7858)
                     /gene="pgk"
                     /locus_tag="ACGI68_003106"
     CDS             complement(6656..7858)
                     /gene="pgk"
                     /locus_tag="ACGI68_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFH0139527.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            complement(8080..9087)
                     /gene="gap"
                     /locus_tag="ACGI68_003107"
     CDS             complement(8080..9087)
                     /gene="gap"
                     /locus_tag="ACGI68_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0139528.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            complement(9147..10169)
                     /locus_tag="ACGI68_003108"
     CDS             complement(9147..10169)
                     /locus_tag="ACGI68_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFH0139529.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
BASE COUNT         3182 a         1910 c         1186 g         4092 t
ORIGIN      
        1 ttttataaaa aatttggttt ttcatctaaa gaaatgagca aaaattataa acttaatgct
       61 agaaatgtta agataaatct taacataaaa ggttataaat ttttacctta tgttaaaaat
      121 tcaaaagcaa gtagagataa cttatgtttt aaaataaaca caaatattga tgagccaaaa
      181 aaatacttag aacaaaatga gattcaaatt aagagaggaa ttgtagaatg tacaggtgtc
      241 tttggtaata tgaaatctat ttatttgaaa aaaactaatg gaaatttaat tgaattatca
      301 aatcatagaa tcacggagag taaaccttgg aaaatttaat cctatcaata aatgttgttc
      361 ttcctttgtt tctcacaatg tcacttggat atgttctaaa aaaattaggt ctatttgata
      421 ctcatacttt aaataaaatg aataatgttt gcttcaagtc tttcttgcca ttattattat
      481 tttttaatat atacaataca gatttagatg gttctcttaa ttttaagctt atggctgtag
      541 ctgttataat tattttttca atcttcttaa tcttagtctt tattattcct aaaattgaaa
      601 aagataataa gacaaggggt gcattaatac aaggtgtatt tcgtagcaat ttcgttatat
      661 ttggtattcc tgtgacaatt tcactatttg gcgataagag tattggagtc acatctttat
      721 taattgcagt tattgtacct atgtttaatg tattatctgt tgtaattctt gaaattttta
      781 gaggtggcaa agtaaatata aaacatatga ttaaaagcgt cttaacaaat ccactcataa
      841 tcgcctcttt tataggattg acaatgctaa ttttaaatat taatatacct acatttttac
      901 acaaaactat tggtgatatt tctaaaatag ctactccttt atcacttata attctaggcg
      961 gctcttttag tttttctgct ataaaggggc atataaaacc cattatattg ggcgtatgtg
     1021 gaaaacttat acttgtacct gctatatttt taccaattag catttttctt ggatttagaa
     1081 atgtagaatt agcttcactt atgataatgt tatctgctcc aactgcagtt tcttcattta
     1141 caatggcaca acagatggat tctgacagtg aattagctgg acaaattgta gtatttactt
     1201 ctggattttc tataattaca gtatttttaa taatatttat tttaaagcag aataatctta
     1261 tttaatgata tttgataaca caaattggta ttataagaaa catatataaa actgatttaa
     1321 tcgcgtaaaa acatattatt atactatttt tactatataa tataactttg tgtatattat
     1381 ctcattaaac atggtgaaag tagtgttgct atatattttt tagtttgaag aaaatatata
     1441 aatacaaaca atatataaat acaaataata aacgccacca aatagtcaat ctataaaata
     1501 aaaactggac taatcatata aaatcattag tccagctttt attttacgta ctttttatgt
     1561 tgatgtactt ttattaaaaa ctaaatttaa tttttaaatg tctagcaaat gcactaatta
     1621 aataatggcc agttattcaa caatatcact acttcttaac tataagtaat gccaatgcat
     1681 ttataaaaaa taatactgct gttatctttg taactctagc aagaaatgct tgttttcctt
     1741 ttggtttaaa gtaagcttga cttccttctc caccaaattc tgaaggtaaa gcattcttag
     1801 aatcttgagg cattatgcta attactagta aaatccctag tactacttgc acacccatta
     1861 aaatgttgct cattcttgcc cctcctatct gtttattact taaatttcat tactctttag
     1921 tgtatcacaa taatatagcc atttcaagac tattatgcta taaataacat acaaaaaaat
     1981 atattgttac tataatatat tactatattc tcaatataaa aatgaataaa acttagaaga
     2041 gaaaactatt taaattaata cataaattaa cacttaaaat aatctactct cattgtatat
     2101 attataaata gccaccaaaa taaaacaatc attttaaaat attgatataa cttttaattt
     2161 caacataatt taaaataaat aatccacatt tagcaataat acttcaatga caattatgat
     2221 taaatcaaaa tatgtcttca cttttatttt ttaataaaaa cctcctatgc aattagataa
     2281 aaatgagata gaaacattta ttcatatttt ttctgaatct atgaataaaa ttaagtaata
     2341 aaatataaaa aaggataagt gaattgaaaa atcaattaac acttatctct tttatatctt
     2401 ataagatgag cttaaatatc ttatattctc tcttcattaa tcttttttag taaccatttt
     2461 aataactatt tttttaagtt atagaaagat tttaatccac aatatctagc ttgctctcca
     2521 accatttctt ctattcttaa taattggttg tacttagcaa ctctatcagt tcttgatgga
     2581 gcaccagttt ttatttgacc agcatttact gcaacagcta aatctgctat agtagaatct
     2641 tcagtttctc ctgatctgtg agatataaca gcagtatatc ctgctctctt agccatttct
     2701 atagcatcta aagtttcagt tattgtacct atttgattaa ctttaactaa tatagagtta
     2761 gctactccat tttcaattcc tttttctaat ctctctgtgt tagtaacaaa taagtcgtca
     2821 ccaactaatt gtaatttatt tcctaatttt tcagttaata atttccatcc atcccaatct
     2881 tcttcgtcta aaccatcttc tatagttatt attgggaagt tgtttgacca atcttcatat
     2941 aaagcaacca tttcagcagc agttaactct tttccttctc ctgctaaaac gtatttttta
     3001 gtttctttgt tgtacatttc tgtagcagca acgtcaagac ctaacataac gtcttcacct
     3061 ggcttatatc cagcttttgt tatagcttca actattaatt ctaaagcttc tctgtttgaa
     3121 cctaagtttg gagcaaatcc accttcgtca cctacaccac aagctaatcc tttttctcct
     3181 aaaactttct ttaacgaatg gaatacttct gctcccattc ttaaaccttc tttgaaacaa
     3241 caagctccta ctggtaatat cataaattct tgaacatcaa cgttgttatc agcatgctct
     3301 ccaccgttta atatgttcat cattggaaca ggtaattgtt tagcatttac tccacctagg
     3361 tattggaata atggtaaacc tatttcatca gcagctgctc tagctactgc cattgaaaca
     3421 cctaatatag cattagctcc taatttacct ttgtttggag ttccatctaa ttctatcatg
     3481 attgcatcta tagctggttg gtcaaatgca tccattcctt ctatttcagg tgctattatt
     3541 tcatttacgt tagcaacagc tgtttctaca ccttttccta agtatcttcc cttatctcca
     3601 tctcttaatt caacagcttc aaaagctcct gtagatgctc ctgatggaac tattgctctt
     3661 cccattgctc catcttctaa tactacttca acttctacag ttggatttcc tcttgagtct
     3721 aatacttctc tagcatatac taattcaata actgacattt gtatatctcc cttcgatttt
     3781 ctattagtta aattttcatt tactgtaaac atccgtctac tattttaatc tactttctat
     3841 attatagtac ataaagttgc ttttgaaaat taggtacaaa tttatttgat gggtaaattt
     3901 gtaaaagttc ctatttttat tttatttttt ataatatttt aatttatata atataaaaaa
     3961 acttgtctaa aatccaaaac caatcttaaa tacacaaata ttataactag atttaaaaca
     4021 ttaaacataa aatgtacaac taatgattct aaaatttttg cttatataat atatattaat
     4081 tattattatt tatattgtta ttgtctatct tgttattatt tatatcatta tttacattat
     4141 tatctatatt gtaatttttt attttataaa ttgtctatct tgtaaatttt ataaaatata
     4201 tcttcatcta caccttacaa tatccagaac ttacaaaatt taaaccttat gtcataaagt
     4261 ctaaacttta caaaataaac ctaaactgca ttatttttaa atttatattg tagtttatat
     4321 attattatct acttatcaga atcgactatt ttgaaataag tgaatgacca gtcatttctt
     4381 ctggcttttc taacttcatc atatctaaaa tagtaggtgc tatatcagat aatcttcctc
     4441 catccaataa ttttgcactt tcatattctt gaccaactac aataaatggt actggattaa
     4501 tagagtgagc cgttacagtt tttcctgttt ctgggtctaa catatattca gcatttccgt
     4561 ggtcagcagt tattattgca cttccaccta actctactat tttatctatc aatcttccta
     4621 cacaagtatc tacagtttca acagccttta tagccgcttc tatacttcct gtgtgaccta
     4681 ccatatcagg atttgcaaag tttaatacta taaagtcaaa cttatcttct cctaatttat
     4741 ctaaagcttt atctgtcaat tcatatgctg acatttctgg ttttaaatca taagttgcaa
     4801 cctttggaga aggtatcaat agtctttctt cacctttatt tggctcttct actccaccat
     4861 tgaagaagaa agttacatgt gcatactttt cagtttcagc agctctaagt tgagtttttc
     4921 catttttagc tagatactca cctagagtgt tggctaaaga ttgaggtcca aatgctatat
     4981 gcacattctc tatagttata tcgtactctg ttaagcatac aaagaagttt ttaatatctt
     5041 ctcttttaaa tccatcaaac tcttcacaaa ctaaagctct agttatttgt ctagctctat
     5101 ctggtctaaa gttgaagaat ataattgaat cattttcctt tatacttcca actggtttat
     5161 catctttcat aataactgtt ggcattataa actcatcatt ttttccatca tcataagagt
     5221 tttgaatagc ttcctcaata gaatttgctt tttcaccaat acctctaacc atggcattgt
     5281 atgcaagttc tactctttcc catctcttat ctctatccat agcgtagtat ctaccagaaa
     5341 ctgtagcaaa ttcacctact ccaatttcat ccatactagc ttgtacttct ttggcatatt
     5401 ctaatgcact ttgagggtca gtatctctac catcagtaaa tgcgtgaaca tacacctttt
     5461 gtacgccttt atcttttgcc attttgatta tagccttaag atggtctata tgtgaatgta
     5521 ctcctccatc agacaaaagt cccattacat gaagagagtt ttcttttgca ttatccattg
     5581 cttcacataa aactttatta gtaaaaaagt caccatcttt tatagatttt gttattctag
     5641 ttaaatcttg atatactatt ctacctgcac ctatgttagt gtgtccaact tctgaatttc
     5701 ccatttgacc atcaggtaga ccaacatcaa gaccactagc atttatcaat gtgtttggat
     5761 actctttttt tattctatct aaattaggag ttttggactc agcaattgca tttcccttaa
     5821 catccttatt gtatccaaac ccgtccataa taattaaagc aactggtttt ttcatcattt
     5881 cactccttag aaattaacaa ggtctaggta atcattacta gctaaactag ctccacctac
     5941 taaagctcca tctatatcac tttgacccat tatttcggct acatttgaag gtttaacact
     6001 tccaccgtat tgtattctaa cttcattagc taattctcca tataatcctt ttataacttc
     6061 tcttatataa gatataacgt cattagcatc ttcagcagtt gcagttttac cagttccaat
     6121 agcccagatt ggctcatatg caacaactac ttttgctaaa tcatctttaa gaacattttc
     6181 taaagctttt tcaacttgaa ctttacaaac atctttagtt tttccagctt ctctttgttc
     6241 taaagtttct ccaacacata atattgggtc tattcctact tctaatgctt ttaaaacttt
     6301 cttgtttaca gtttcatcag tttcattgaa gtattgtctt ctttctgaat gtcctattac
     6361 aacatagtcc atatctattt cttttaacat aagaggtgaa acttctcctg taaatgctcc
     6421 tttttcttca aagtgcatat tttgagcacc tatctttata tttgtaccct tagtagcttc
     6481 ttttaaatcc tttaataaag taaatggtgc acatataaca gcttctactt tatcagaatt
     6541 tactttatct ttaacttcat ttacaaattc taaagcttct tttattgttt tatgcatttt
     6601 ccagtttcct gcaattatag gttttctcat tttacaatct cctctatata ttagtttatt
     6661 tattatctaa tgctgcaata cctggtaatt ctttaccttc taaaaattct agtgatgctc
     6721 ctccacctgt tgatacgtga gtcatcttgt caccaaatcc taattgatta actgctgctg
     6781 cactatctcc accgccaatt acagtagttg catcagtcaa ctctgccata gctctagcta
     6841 ctgataatgt accatttgca aagttttcaa attcaaatac tcccattggc ccattccata
     6901 ctacagtttt tgattctttt atagttttta caaagtttgc tatagtctta ggtcccatat
     6961 ctagtcccat gtaatcttct tttacattag catcttctgt tactattgga gtagcatctg
     7021 gtgcaaagtg gtctgccata acaacgtcta ctggtaataa taagttaaca cccttattct
     7081 tagctttttc catcatttct ttagcatatt caactttatc tatttcaagt aaagaagttc
     7141 ctacttcata accctgagct tttaagaatg tataagccat tcctccacct attattagat
     7201 tatcaacttt ttctaataat tcgtttataa cagctaattt atcagaaact ttagctcctc
     7261 caagtatagc tgtaaatggt cttacagggt ttgcaacagc ttctcctaag aattttaatt
     7321 ctttttgaat taagtatcca caaactctct cttgtaaaaa ctcgcctgca ccaactgttg
     7381 agcaatgtgc tctatgagca gttccaaatg catcatttac aaatatttca gcaagtgatg
     7441 ctagttcttt agagaaattc tcttcgtttt tagtttcttc ttttctatat ctagtatttt
     7501 ctaataatac tacatctccg ttttccattt tttcagttgc tttctttgca ttttctccta
     7561 caacattatc gtctgctgca aatacaactt cttttcctaa catctcagat aatctctttg
     7621 ctactggtgc taaagataat tctggtttag cttctccttt tggctttcct aagtgagagc
     7681 ataatataac ttttgcccct ttagaaatta aatattgaat agttggtaaa gctccgttca
     7741 atctattttc atcagtaatt acaccatctt gtaatggcac attaaagtca catcttacta
     7801 atactttttt tccgcacaca tcaatatctt ctatagtttt tttgttaagc attgacataa
     7861 atatctctcc ttttaataaa gtaatttttt attgccaagt ataatatagt acatactttt
     7921 accctattaa atatatcata ttatcatatt tttatatata gtatgtaaca ttatttaggt
     7981 gtattttata tattttttct taaaaaaagt cctgagtccg tagtattaaa ctacaaactc
     8041 ggactttgtc aatctacttt tctattttct caaaactgac tattttgcta attgtgcaaa
     8101 gtatcctaaa gttcttatta attgagaagt ataagacatt tcattatcat accaagaaac
     8161 aactttaact aattgttttc catctacttc cataactttt gtttgagtag cgtcaaataa
     8221 tgaaccgtag cttattccga ttatgtcaga agatactaac atttcttcag tatatccgaa
     8281 agattcgttt gaagcttctt tcatagctgc atttatttct tcaactgtaa cattttttcc
     8341 taatgtacaa actaattcag ttattgaacc agttacaact ggtactcttt gtgctgcccc
     8401 atctaatttt ccttttaatg aaggtattac taagcctatt gcttttgcag ctcctgtagt
     8461 gtttggaact atgtttccag cagcagctct agctcttctt aaatcacctt ttggatgagg
     8521 accatctaaa gtattttggt cattagtgta tgcatgtata gtagtcatta agcctttttc
     8581 tataccgtat ttatcattta atactttagc cattggagct aaacagttag tagtacaaga
     8641 agctcctgat ataactgttt cagaaccatc taatatgtca ctgtttgtgt tgaatactat
     8701 tgtttttaaa tctccagtag ctggtgcaga tataacaact ttcttagccc ctgcttgtat
     8761 atgagcttca gctttatctt tagatgtaaa gaatcctgta cattctaata ctatatctac
     8821 tcctaattca gcccatggta ggtcagcagg atttctttct gctgttactt ttatttcttt
     8881 tccgtttact acaaaagcac cttctttaac ttctatttct ccgttaaatc ttccttgagc
     8941 tgtatcatat ttaaataaat gagctaacat tttagcgtct gttaagtcgt ttattgcaac
     9001 aacttcgaac ttatcttgtt gctccatcat ttttcttaat gctaatcttc ctattcttcc
     9061 aaatccattt atagctactt taaccatttt cgctacctcc tataaagtaa atgtatgttt
     9121 tctagctctg tgctaggtct tgacttttat aataatgaca atatcttata agcactttct
     9181 tcatctgtta caagaaccat gtttttatgc atatttgata tagctaataa ggcttctact
     9241 ttttcagtcc ctgcaaagat tgcaatagtt tcttttactt gtttaactgt ttctaaatca
     9301 attccaacag tgttcagttt gtaaactatt tctccatctt tgttgaaata atatccaaat
     9361 gcttctccaa ctgcattttt agataatatc tcatcaacct tgtcttctgg aagttttctt
     9421 ctctttgcca tgacatctgc tctacctata ctaaaaacta aaatgtctgt tcttttaata
     9481 tactccaaag ttgtcttaat ctctggcact tggctaagag ttttcattgc actttcatcc
     9541 aactcatctg gtatatgtaa aagtttgcaa ttagaatgta atttcttact tattgaagca
     9601 actatattgt tagattgcat ctctacatca gttcccatac ttcctctagc tggaactatt
     9661 gtagagtttg gatatctctt atcagtttta ataacgtttg aaaactccaa catagtgctt
     9721 cctcctgtta ttgaaacgat atctccatct tttaaaatgc ctaagaaata ttcgcatcca
     9781 tatctagtta catcttttag taaacttcca ttttcatcac agcttccagg aactaatatt
     9841 acccttttta ttccaagctt ttctctgact ctatcttgta acttagacaa tcccatgatg
     9901 tcactcatga tgtccttaag tttatctaac aaatcagaac cttctttagt gatagtcatc
     9961 ccagatacag ctacgtctat aagtccttgt tcttttaaaa actcagtttc tgacctgatt
    10021 attctttcac taatctctaa tacatttgat aatgttctcc tacctattgg ttcacttaaa
    10081 gatatttgtc ttaaaataga gtatcttctt tccataagtt caattgcctg tggaatcaat
    10141 ttcttttgaa tttctaataa gttcttcata acatactcct tattgctttc ctttcgggac
    10201 tttttttgtc cctagtgttt atttaagtcc cacaagatat ttttatttta taggatttgc
    10261 gtatagattt caatagtttt tcaaaaactt ttttattttt ttattttata aaatatttct
    10321 ccaccatttt caaatcacag tattttttct ttttatataa ggctttttat
//
LOCUS       DAYASV010000133        10277 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYASV010000133 DAYASV010000000
VERSION     DAYASV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.136"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(82..681)
                     /locus_tag="ACGI68_003109"
     CDS             complement(82..681)
                     /locus_tag="ACGI68_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viroplasmin family protein"
                     /protein_id="HFH0139530.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(699..3818)
                     /locus_tag="ACGI68_003110"
     CDS             complement(699..3818)
                     /locus_tag="ACGI68_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFH0139531.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANKIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINFIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDVPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKSRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4039..4863
                     /gene="pdaA"
                     /locus_tag="ACGI68_003111"
     CDS             4039..4863
                     /gene="pdaA"
                     /locus_tag="ACGI68_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HFH0139532.1"
                     /translation="MKKDSLKKYIMIGAFALTLFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(4960..5745)
                     /locus_tag="ACGI68_003112"
     CDS             complement(4960..5745)
                     /locus_tag="ACGI68_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139533.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEGGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5845..5979)
                     /locus_tag="ACGI68_003113"
     CDS             complement(5845..5979)
                     /locus_tag="ACGI68_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139534.1"
                     /translation="MNISRKAMKIIELAQKIANKRGISVEEAWSEAVTEYKNKYEHIA
                     "
     gene            6210..6575
                     /locus_tag="ACGI68_003114"
     CDS             6210..6575
                     /locus_tag="ACGI68_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0139535.1"
                     /translation="MKIRAEYTCPLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6653..7207
                     /locus_tag="ACGI68_003115"
     CDS             6653..7207
                     /locus_tag="ACGI68_003115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFH0139536.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIIIKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYYK"
     gene            7427..8101
                     /locus_tag="ACGI68_003116"
     CDS             7427..8101
                     /locus_tag="ACGI68_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFH0139537.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      8456..8552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8606..8782
                     /locus_tag="ACGI68_003117"
     CDS             8606..8782
                     /locus_tag="ACGI68_003117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139538.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(8953..9192)
                     /locus_tag="ACGI68_003118"
     CDS             complement(8953..9192)
                     /locus_tag="ACGI68_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139539.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(9307..10146)
                     /locus_tag="ACGI68_003119"
     CDS             complement(9307..10146)
                     /locus_tag="ACGI68_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0139540.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKMYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITKYLYFNGL
                     DGK"
BASE COUNT         3567 a         1582 c         1022 g         4106 t
ORIGIN      
        1 tttaattaca agttgtttaa ttataaagta ggaatttaga tatactctta taccatgtaa
       61 ttatgtataa atattttttt attatacacc aatagctttt ttagctaatt tatcagcttc
      121 ctcattatac ttatttccag aatgagcttt aacctttgta aattttacat taagcttatt
      181 cttaatactg tcatagaaat ttttatatgc tatagttcct tctttatttg ttttccaaac
      241 tccagtacac cacttttcaa tcccttcata gtcaaagtat aaaatcaagt tttgaacatt
      301 attgtcaata cagtactgca ttgcaatttt agaagcctct atctctccag ctacatttct
      361 catacttact aaagttttct cttttccttt ttcactataa gtttttataa cttcattatt
      421 ttttaaaatc acaacacccg aaccatacat ttttactgaa tgttcataac ttccatctac
      481 atatgcttct ataaactcct tatcttcttg aaaactcaat ttttctgacc catcaatata
      541 ttctttagct tcttgaaaag tagaaaaact cttatattca gctcctgaga atccatttac
      601 ttgttttttg cactcatccc atgtattata tacacctata tttttacctt ttttcacagc
      661 ataaaacttt tgttttgcca aatatatcac tcccactctt aatttttatc ttcttctctc
      721 ataaatatat ctaaatttat aagatagttt ctatcaaaat attttacttt catatctttt
      781 tttataatat caatatggta cacttctcct tttacagctc tttgtcttat attttcattt
      841 atatcttttt ttataatatc cttatcaaca tcattgttat ccattttagc atcaacattc
      901 aacgctttta tagtatcaaa tataacagat tttagtatta aacttctaga tttatttaaa
      961 tgtcgcctta tataagtata aaactcattg ctattaacct ttgaaatttt acaatcgtgt
     1021 aaatctttct ctaatctctt taacacactt atattagttc ctacatcaaa aatcacaaaa
     1081 catccatagt catatttgct tcttaaaatt ctccccatag cttgctttac ctttatggtc
     1141 atttgaggat aatttatagt atagtagtct ataccatact tcttcattat agtaaaatac
     1201 aaagggtctt taggatttag attcggaagc ttgtctaaag ttacacaaac caaaccatcc
     1261 cctggaacgt ctacgccttc aaaacatcct tttgagccaa gtactacaca ttttctattt
     1321 aaatcgttaa ggtgtctaat gcccttttta tcagcataaa tttctaagtt aaaactatgt
     1381 aagtattttt tcaatatttc ataagttttt tcttgtctat ctttactatt aaaaagagct
     1441 aatgtatgac cttctgtaat ctttgatata ttagatataa ttttactcat ctcatttgga
     1501 aattcagaat ttctgtactc acaaatatca gaaaatccaa ctacagagac tctattttta
     1561 taatcaaaaa ttggttctat aatcttttcc acattcttaa ctctgtctat tcctaatgta
     1621 tttttaaaat aagacatact ttcagataaa ctcaaagttg ctgataaaaa tattcctttt
     1681 tctaattgag ataaaatatt ttcttcaaat aaatctgcta tttttaatgg tactactcga
     1741 aattcaaagt cattataatt cttatcaatt tcaactatcc ttgcataatc atccttttca
     1801 tcatactcta aaaagatttc aaatataatc tttatatcct ctaaatctct tgtctttgcc
     1861 tttccaaatt tatatatatc tgcttcttta tctatactat catcatcaat attcctataa
     1921 attattatta ttattgaaac taaatttttt attattttct cacaactcaa ctttatcttt
     1981 tcactatagg ctctataact tatttcagta tctataccat ctttttttag ttttcccaca
     2041 atttcatcta tttgcaaatt cagttcccat cttaaattat aattacttac attattatat
     2101 tcattacaat taccaaactt taatattgaa tctatctctt caataatgaa atttatactt
     2161 ctagcaattt tttgtttatc ttctcttcca atttttaata cattataaaa tttatcaaaa
     2221 gcctttattt ttctcatatt tctactatat tttttatata taaagctact attttgaata
     2281 aactcgtaag gataaatttc ttgtaataag taccttaaag actttgaatt tataatactt
     2341 gaaaagaaat catatccttt ttcagtcaaa ttatgtgctt catctactat tatattttct
     2401 aaaggttttt catccttata cggccattta gctaatagtg aatgatttac tactgttata
     2461 tgttcttctt ttaattcttc tattctgttt ttatataaac aatctttttt gcatttttta
     2521 ggtctgcaca tatttgggtc acaacttaca tttcttaagt gagtacttat ttccttgaag
     2581 ttgtcaaaca ctaaataatt tatttcttct atatctccat acttgccacc ttcaactaat
     2641 ctcttcaaaa atattaaaga taaaatttct cctttagtag ggttttgaga ctcataatca
     2701 tctatatatg cttccagtct atctatacaa atatagttat tttttccctt tatatatccg
     2761 taacttactt ttccatttag acccaaagaa tctaacacat ttggaatatc tttatttatt
     2821 aattgtatct gcaattcctt tgtgtcagta gaaattaata atcttttttt attaattcta
     2881 gcctctaata ttgcaggcaa taaatatcct acacttttac ctataccagt tggtgcctct
     2941 atacatgcaa ttttttcttc atcttcgcta tttctaaaca actctcttat tgttttagta
     3001 agctcatatt gaccaggtct atattcatat gtaaatcctt ctttattctc ccaaatagtt
     3061 ttgtatttta ataattcttc ataatttttt tcttcttcat ttaaaatttt taaggtttct
     3121 ctttccttct tcttttcttc ttttgagtca aatatattat attctttttt tatatttata
     3181 ttatttgata aatcataatt tgcctcttct aaaaatttgc tccattccca tgttggaaga
     3241 ttaaattttt taagatatga atttatttta aaactcaatg gctccaaagt agttttttca
     3301 tagttattaa attttactaa aaggcaattt acaatattta ttatatcaat agcatctgat
     3361 aaagctctat tttccacttt agaattacaa tttgttaaag tattttttaa gtattctaag
     3421 ctataatctt tatgatatgg ttctaaaata actgctaatt caattaaatc aataaattta
     3481 ttttttaact ttggcatatg atactcaaaa aacttttttt tcagacttaa atcatgacat
     3541 ataatgaaac tatcttctac aaaatttaca agtctatctt ccaccagttc tattttatta
     3601 gctatttcta aatcattttt atctaaattt ttacatactg agaatacttc tataggtacc
     3661 tcaaatttgt tttttattaa tgattcatag gtatatattt tccagtcttt aaccttgaca
     3721 gctccaactt ctaaaatttc tgaatttaaa caatctaatc cactaacttc tatatctaaa
     3781 aaaacaacat cattcaatac acttattata ttatccattt ttatatccct tcaagttaaa
     3841 ccagcctaac ttatttacaa tcatttctct ttatatttta tcatttatat acactttatt
     3901 taagataatt aatatcaact ttatatctta cagtaaactt ctttcattta ttatttgtat
     3961 atgtgaatat ttttaactat aaatattcaa actatattat aagaaatata ctttaaattt
     4021 taaataagag gtgaaattgt gaaaaaagat agcctaaaaa aatacattat gataggagca
     4081 tttgctctaa cactttttgg aattgctagt ataaatttta aatcattaga taaaacaaaa
     4141 actcaaattt ctagtccaac actcgacaca catgaatatg attggtattt taaccctagg
     4201 gaagatggta aacagccatc acctataaaa gaagctgatt tttttaaaaa atacggagct
     4261 tattatgtag gaaatcccaa tgaaaaagtt atatatctat catttgatgc tggatatgaa
     4321 agtggaaaca ctccaaaatt attagatacg cttaaaaaac acaatgctaa agctcaattt
     4381 ttcgtagttg aaagttatat aaaaagcaat ccagagttaa taaagcgtat ggaaaaagaa
     4441 ggacatttag tttgtaatca ttcaaaaagc catccatcaa tggcaggtat tacagatttt
     4501 gaaaaattca aagaagaaat tacaagtgta gaaaaagcat acaaagatgt tactggaaaa
     4561 gaaatgccta agtattttag acctccaatg ggtaaattta gcgaacaatc tttaaaatat
     4621 acacaagatt taggatataa atcaatattt tggagttttg cttatgtaga ttggtatgaa
     4681 aagaaacaac ctactcatga atttgccaaa aacaaaattt attcaagaac tcatcctggt
     4741 gcaatagttt tactgcatcc aaattcatca acaaatactg agatattaga tgaggttctt
     4801 acacattggg aaaaagaagg ttataaactt aaaacacttg attatttaaa taataaaaaa
     4861 tagtatcata aatttaaaaa aactctatca tgaattatag taaaaatata gttatttata
     4921 aatactttaa ttctcgatag agttttttat tttttatagc tatttaaaaa gaattgtcca
     4981 ttcatttatc tctttgtatc ctatattatg atatatttta cctgctacat cattattata
     5041 aaaaagacat ggaacttttc cttctaacaa caattcttta ctcaaattgt aaaccatata
     5101 acttgcaagt ccttttcccc tatatctttt atctgtactt acagagacca ccattgccaa
     5161 aaaactagtt tccataccag ttgacacagt gctaatcata gtatcatcat ttctaataaa
     5221 atatgctctt acatttccct cttttaaatg aacttttctt tcttctatgt aatttttact
     5281 tactttatag tctgacctat ttaaaagttt tcctatttca tcaatatctt tttcctgagc
     5341 attttctatt ttatactcct tcatgttgga aaaatctatt tcttttattt cttttaatac
     5401 acaaaattta ttatctagtt tttcataaaa atctttgtat ttacttatta gtctatctat
     5461 tacacatttt tttccactta tgctttctac atctaaatct tttatatgat tcttcatttc
     5521 tcctatatta aaattatttt taaaactata aattataagt gttgacttat tttttaatat
     5581 tatagaggtt atatttccac cttctacctg agaccatata tcttgatatt gagagctaaa
     5641 tccataattc tcaacgtatc caattatgaa taaatttaaa ctttcttcat gtgatacata
     5701 ttgccaaaat tctttttcta aagtattatc tatttttaca atcaagcaga tatcctcctt
     5761 ctatgttaat aatgatatac cttataattt atagtatcat ttaaaatcat tatattctac
     5821 caaatattct aaatttttaa atatctatgc aatatgttcg tatttatttt tatattctgt
     5881 tactgcttca ctccaagctt cttctactga aatacctctc ttatttgcaa ttttttgtgc
     5941 taattctatt attttcatag cttttcttga tatattcata tatattcctc ctctaaatta
     6001 tcataagtta ttttaaagtt aaatttctat agtcatgtta tatatttatt tacttatatc
     6061 ttactaatta tttatgtact aaatatgaac tacaatagaa gaaatttaga acttaataaa
     6121 acataaaatt tatatacagt tcatatatat ttgtcatatc acaagacaag tgatataatt
     6181 tattatcatt ttactggggg tgatatgcta tgaaaataag agctgaatat acatgtcctc
     6241 ttgaaattgt acatgacata atcaaaggaa aatggaaaac tatccttata tttgaattaa
     6301 gacaatctaa taaaacattt tctgaattgc aacataatat ttatggaata aatcaaaaaa
     6361 tgttgattca acaattaaaa gagttacgtg attttgggat tattgataaa ataagttctg
     6421 ctggatatcc attacatgtt gaatattttc ttactaatcg tggtaaaaaa atgttaaaag
     6481 cagtggaaat aatgcaagaa ataggaatcg aatatatgct tgaacatggt caacaagaaa
     6541 ttctagataa taaaggaatt tgctacaata agtaatccta tacataccaa aaagtaagta
     6601 atttacattt tttacaatag tattataata aaaataaata ggaggtataa tcatgtctat
     6661 agctttaata ttgattgata ttcaaaatga ttattttaaa aatggaaaat gtgaattatt
     6721 tcaatcagaa gaaactgctg aaaatgctaa aaaaatacta ctgtttttta gaaaaaacaa
     6781 tttaccagtt attcatatac agcatatatc aacagataaa acagcttctt tttttcttcc
     6841 taatactcat ggttcagaaa taaataaaat tgtttttcca ctagataatg aagaaattat
     6901 aattaaacac acaccagata gtttctttga aacagatttg caaagtatcc ttgagaaaaa
     6961 aaatattact aatctagtaa tctgtggaat gatgagccat atgtgtattg atacaagtgt
     7021 aagaacagca aagaaacttc gatacgacat aactctaata agcgatgcat gtacaacgaa
     7081 gaatttatct tggaataata tagaaattaa tgcaaatact gtacatcaag tattcatggc
     7141 atcactacag aacagttttg cagatgtaaa aaacacagat aaatttttat ctaactatta
     7201 caaataaaaa catacactat gttaaaagag caagtttgat ttatttaagc ttgctcttta
     7261 atcatttaaa attagatgct ttctatcatt tttatataca taatatacgt ttatacattt
     7321 tatattcata tataactatt atataaattt taaactttac tatattcaca taaaaaatgt
     7381 agtattatag tatatataat attatataga agggggtgct agataaatga accatcatat
     7441 attgattcaa tttgaatata tagcaagaat cattactgct ggtttctgtg gggctttaat
     7501 tggatatgag agaaagaata gactaaaaga agctggaata cgcactcatt ttatagtggc
     7561 attagcttca gcacttataa tcattgtttc caagtatgga tttaatgata tagtgggtac
     7621 tccaggtatt ggtctagacc cagcccgcgt tgcagcacaa gttgtcagtg gtataggttt
     7681 tttaggtgct ggattaatat ttattagaaa tcaatcagta agtggtctta ctacagctgc
     7741 tggtatatgg gcaacagccg gtacaggtct tgccattggc tctggattat acttagttgg
     7801 tattgtttca agtatcatta ttgttattgt acagataaca cttcatagag ataggaaatg
     7861 gatgaaactc cctacagcag agcaagtttc tcttaaaatt tctaacagca ctgattccat
     7921 agaatatatt gaaggtaaat ttaaaaaatc taatattgaa attataaaca tgaatattaa
     7981 acgccttgat ggggattggt tagaagtagt tatatatact aagttaccta aaggatataa
     8041 aaaaacaaat ttattaaaat tatttagcga taatcctctt atagaaactt tagaaatata
     8101 atataaaaat tgaagcttaa aaataataat atatttaatc aaggtcaata tataaataat
     8161 taagggatga gtctatataa aattcatccc ttgttttttg ccattcatat atattttttc
     8221 aagatatttt tgtttatcta ttatctagcc aagtaaccct tgtgaactaa actttttaat
     8281 acaaaaactt caatacaaaa ctaaatataa tttttattaa acattctata catatagttt
     8341 gtttcttgag tgaaaaatag atttcatatt gttaagatat tgttaaatat ttcaattttc
     8401 tgacaagtat gttgaacaat aacttatcct agtgtattat tttagttgta gaataaaact
     8461 aaatcggcaa aactagagaa atttagtgac gcaaagctat agggactaac gcttataaaa
     8521 taaactatga gttatgtcag ccagttgcca aaaagatatt gttctttttg ttttatgaaa
     8581 gttttttagg gggatagtaa tatatatgtt tagagatgaa atggataaat gtacacacat
     8641 gttaactgct tatattagta gtttatatga ttattgtgat tttatagata cacagctaga
     8701 tgattttata ctagagtacg gagaaaatgt agtagaatct tgtttacatc aagtgatggt
     8761 attggtaagt aagtataatt aaatagaaat tgttttctag agaacccttt ttagttttat
     8821 ctacatttaa aaatgttatt atattaaaaa actaatcttc catatatgca aagttttctt
     8881 ccatataaca atttcttccc atttatactt tttcacttgt atctttaata aaattcaact
     8941 tcaaaaatta ttctatttct tatctttaca ccaattccta tcagtattat cctttatata
     9001 ggttctaact atttcgccac aatctaaaca taaatctgaa tatatagtag attgtactgg
     9061 aaaagtttta tttataactt tatctaattt tccatctcct ctatatttta atccaatagt
     9121 ttcagcatat atgccaccag ttgttaaaat tcccttttca atatttacac ttccacaatt
     9181 agaacatttc acatttataa ccccctaaac aattattttt tcatgctatc ttataattta
     9241 aaatataatt tgctaccgat tttataacac agtggcaaat cggtgccaaa tcaacatttt
     9301 ttcattctat ttaccatcta atccattgaa atatagatat ttagttatag gaacttgaat
     9361 ttctgttaca aactcctcta caattccagt ctcatgtata tctatcttat agatttctat
     9421 agggtcagca attatcttat aattattttc ttctataaat ttaaacacct tctccatata
     9481 catcttatta ttagaattac tcccagtata atttaaagtt acataatatc cttcatcaaa
     9541 aactatatta tatactttct cattactttc cagaaaacaa aatacagatt taaactcatt
     9601 aaatatgcct ttattaattg cttcagtaga aaaggcagaa ccaatattgt tgtttccaag
     9661 tatattaaat ctatcttcat attctttctg aagtttttga attaaaaaat cgaagtcttc
     9721 atccctcttt atatcagcat ttaattctaa agcttttctt ttttctatat atacaacctc
     9781 tatctcatct atttttgtat tttgaattac actttcaatt gaactcaatc tttttataat
     9841 attttcctta tggcttataa attcagctat ttttttatca attaaatcga gttcttcatt
     9901 taaaatattt ttcgtagact ctatacttct gtcatctagg tattctttta ttttcttcat
     9961 tggaaagttc aagcttctaa attcttttat taaatttaat ttccatatat ctgatatatt
    10021 atacattcta tatccattta tatctctaac tggtcttaga atgcctattt cttcataata
    10081 cattaatgaa tctctaccta ttccatatat tttagatatc tctcctattt tatagtaatc
    10141 tttcatttat ataataaact cctccgaaat cttattttta ttttaaatca acattattac
    10201 tctataatac tatagtatta gaatatacta aaaaaatctt taaaacaagg ataggaaaat
    10261 aaacatccta ataatta
//
LOCUS       DAYASV010000134        10185 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYASV010000134 DAYASV010000000
VERSION     DAYASV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.137"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..1335
                     /locus_tag="ACGI68_003120"
     CDS             <1..1335
                     /locus_tag="ACGI68_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HFH0139541.1"
                     /translation="LWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYILKNYYQ
                     DNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDHFILLI
                     ACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLLIDRAN
                     IAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYSLDDVN
                     EIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWINKGYT
                     PVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILIDIMKK
                     IKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISKIVEMA
                     KAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            1482..2228
                     /locus_tag="ACGI68_003121"
     CDS             1482..2228
                     /locus_tag="ACGI68_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139542.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            2228..4108
                     /locus_tag="ACGI68_003122"
     CDS             2228..4108
                     /locus_tag="ACGI68_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139543.1"
                     /translation="MDFRKFAYNNIVKNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGVII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAQITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKIYTRKVWF
                     FD"
     gene            4227..5618
                     /locus_tag="ACGI68_003123"
     CDS             4227..5618
                     /locus_tag="ACGI68_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0139544.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            5725..6765
                     /locus_tag="ACGI68_003124"
     CDS             5725..6765
                     /locus_tag="ACGI68_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0139545.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            7254..7907
                     /locus_tag="ACGI68_003125"
     CDS             7254..7907
                     /locus_tag="ACGI68_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFH0139546.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKAAEKIDGIIDGMKF"
     gene            7983..8192
                     /locus_tag="ACGI68_003126"
     CDS             7983..8192
                     /locus_tag="ACGI68_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139547.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(8332..8898)
                     /locus_tag="ACGI68_003127"
     CDS             complement(8332..8898)
                     /locus_tag="ACGI68_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFH0139548.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            9059..9712
                     /locus_tag="ACGI68_003128"
     CDS             9059..9712
                     /locus_tag="ACGI68_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFH0139549.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGFSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(9870..10046)
                     /locus_tag="ACGI68_003129"
     CDS             complement(9870..10046)
                     /locus_tag="ACGI68_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139550.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         3969 a         1003 c         1626 g         3587 t
ORIGIN      
        1 ttgtggaaat taagtaatgc taaattaata gaacaactta aaaaattggc ttattatgat
       61 tctttaagtg gaataaaaaa taaagaaaag tttagaaaag attctatgta tattttaaag
      121 aattattatc aagataattt ttacttagta caattggatg tcaataaatt taaatatata
      181 aatgagatgt ttggatatgc tgaagggaat aagatattaa ttcatatatc acaggtttta
      241 aataataata caaataaata tgaaatttgt gctaggatgg ataatgacca ttttatattg
      301 ttgattgctt gtaacactga agatgaactt ttaaatagat tatctaaaat aaataaagaa
      361 atttgtaact taaatactac taactcatct aagtataaaa ttgttatgtc aagtggaata
      421 tataagatta ctaaaaagga tgatattaaa aagattgact tgttaattga tagagcaaat
      481 attgcagcaa aaagtaaaaa ggaaaaatat gaacattcat attcattttt taatgaagat
      541 acaagaaatc gattgtacaa ggaaaaacga ttggaagata atatgaataa agctttagaa
      601 aaaggtgaat ttatagtata ttatcaacct aagtatagtt tagatgatgt aaatgaaatt
      661 gaaggagcag aagccttgat aagatggaat agtccagaat ttgggtttat ttcacctata
      721 gattttgtac ctttatttga aaagaatggt tttattgtaa atattgatat gttcgtgttt
      781 gaagaagtat gcaaaacatt aaacaagtgg ataaataaag ggtatactcc tgtaccaatc
      841 tctgtaaata tgtctagagt tcatttatat agggataatt ttatagaaaa tataactgat
      901 ttgataagta aatacaatat atctcctgag tttattgaac ttgaactaac agaaagtgtt
      961 gtgtttgata atttaaatat attgattgat ataatgaaaa agattaaaga gataggattc
     1021 cttatatcaa tggatgattt tggttcggga tattcatctt taaatctgtt aaaagacctt
     1081 tcttttgata ttttaaaatt agatagagga tttttaatcg aaacaacaga tacaaaacgt
     1141 ggaaaaatta ttatttctaa gattgtggaa atggcaaaag cgattgatat taaagtaatc
     1201 tgtgagggcg tagaaactta tgaacaagta gagttcttga aagagatagg ttgtgataag
     1261 gttcaaggat acttatttgc taaacctatg gtattagatg aatttgaaaa acacttaaac
     1321 tttaaatttg attaatcatt aataattatt agactataat ataccctaac aacttactta
     1381 caaatatgta agttaattgt tagggtattt ttatggttaa agtagtatat tcacgatatt
     1441 atggtattgt agtaaaaata aaaaattatg gagaatataa aatgaacata ttgagtatta
     1501 gaaatatatc aaaaacatat tgcggaaata ttccatttaa agcattagat aaggttagtc
     1561 taaatataga aaagggagaa tttgtgtctg ttatgggacc atctggaagt ggtaaatcta
     1621 ctcttttaaa tataatatct acagttgata gacagagtga aggtgaggtt gtattagatg
     1681 gatatgatgt aagtaaattg aaaggagaaa aacttgctga atttagaagg aaacaacttg
     1741 gttttgtatt tcaggatttt aatttgattg acactttaac tgttggcgaa aatattatgt
     1801 tgccacttac actagagggg gcaagtatca aagatatgaa tatacaaact aaaagtatat
     1861 ctaaattttt aggaatagat aaaattttag atagaaaaac ctatgaaatt tcaggaggtc
     1921 aagctcaaag gtgtgctatt gctagagcta ttataaataa acctgccata cttcttgcag
     1981 atgagccaac tggaaatctt gattcaaaat ctactgatga tgttttgaaa ttatttacta
     2041 ggataaacaa agaacaaaag gtaacaacac ttatggtaac tcatgaagca tatagtgcaa
     2101 gttattcaga tagagtaatt tttataaaag atggttgtat atatacagaa ataaaaaaat
     2161 ctgaaagcag taattctttc tattctgata tactagctgt tttgtctcaa ataggaggtg
     2221 taagatagtg gattttagaa agtttgctta taataatata gttaaaaatt tcagggcata
     2281 tttagcatat tttttaagtt ctgttttttc tataatgata ttttttgttt ttgcagtgtc
     2341 tatgttccat ccaattataa caaaatctgg aatgcagagt ggttctactg catttatggc
     2401 tcttatgact tcagaattaa taatattcgg ttttagtcta ctttttatat tgtactcttt
     2461 aggaaatttc ttgaaatata gattgaaaga ttttggagta ttgatgataa ttggaatgag
     2521 taacagacaa ctgaaaaaat tgatattaat tgaaaatttg ataataggat ttttagcagt
     2581 gatgttagga atattacttg gtttatcaat ttcaaaattg tttttattgt atttaagcaa
     2641 actgttttat atgaatttaa ctgaatttta ttttccagta aaagcaatgc tattgactat
     2701 tgtatcattt atgattttat ttttgataac aggaccaatg agcttaaaat tattgagtaa
     2761 aaagagcatt ttagagcttt tagtaggtac aaaaaagcct aaaaaagaga taaaatcttc
     2821 aaagctattt ggaataattg gtgtaatatg tcttatatta ggatatgcta tgatactatt
     2881 tggcaataag ttaaaaataa atggaggtat gtatacaaca gcactattaa ttactttggg
     2941 gatattttta tttttttctc aatttactat tttgatatta aagtatttaa aaaataaaga
     3001 aaaattttat aagaacaaag taaatatgtt acttataagt aatttgatat ataaattaaa
     3061 ggataataca cgattgttat ttttaataac aatactttta tcggctactt tagtagcatt
     3121 tacaacaact tcaacacttg taagttctca aggagaagct tctaaaaata attttccaat
     3181 ggtttactca tatttttcag agcataataa tgaaaaagaa tatgaagaac tacaacaaat
     3241 tagagaagca atcaaaggat atgattataa ggagttaagt tttcctgtac ttaaatataa
     3301 tagggaagtc ttactaagtg taaagaatta taacaaatta tcaaaggaat tgggattaga
     3361 taaaattagt tcaaagttag aaaaatatga aggggttata attcctagta gcaattcaca
     3421 agaacatata aattctttaa aacaattaaa aaattatagt ataaaaaatt taaatataga
     3481 agtaaaaaaa tcaaaaaata aaataatttt tcctacagga ctgtttagta aagttgtagt
     3541 tataagtgat gacttataca atgaagtaaa aggtgaattt tcaaaaatta attttcatgg
     3601 atttgattat aaaaactggc aaagcagtgc acagataact gaaaatttaa aagaaaaaca
     3661 ttttaatttg gatagagaat ataacaggtc atatttttta acattaccag atatgtattt
     3721 agtagagtta caacaaagta aaattcttca atttatgggt gtttttatag gagtgatatt
     3781 atttatagca gtttttagtt ttttatactt tagattatat acagacgaga tagaagataa
     3841 tgtaaagtac aaaaaactat caaaaatagg tctatctttt aaaaatatga ataagattgt
     3901 tagtatagag ataggtacat tgttttttat accgtatttt atagcaatta taaatagcat
     3961 aatttcttta ctgtttttaa atcaagtatt aaataatagt tttaagttag aaaatttggg
     4021 tatattacta ctgatatcat ctatatattc actttatttt tacttattaa aaaaaattta
     4081 tactagaaaa gtttggtttt ttgattaaag tggtatataa tagatatata aattaatatt
     4141 taaaaagtag ttaatattag gggagggcat ttgcacctcc cttgtaatat gtggaaaatt
     4201 gaatattaga agaaggagga gacttgatgg aaaatcaaca attattagga acagaacgaa
     4261 ttagtaaatt actactaaaa tattctatac cagctataat aggtatgttg gtaaatagtt
     4321 tatataatgt agtagataga atttttattg gaaatatacc aggggttgga ccacttgcaa
     4381 taacaggtct tggtgtaaca atgccaataa tgacaataat acttgcattt gggatgttga
     4441 taggtatagg aactactact accatatcaa taaaacttgg gcaaggtaaa gtagaagagg
     4501 caagaaaact tataggaaat gcaatgacgt tatctgtaat aacaggaatt atcataatga
     4561 tacttggaat attatttgca aataaaatac ttacattgtt tggagcaagt gaaaatactt
     4621 taatatatgc aaaatcatat atcaatatta tattattagg tacagtagta aatcttcttt
     4681 ccttttcttt aaaccactca ataagagctg atggtagtcc taaaatttca gcaggaataa
     4741 tgatagtagg atgtttaact aatatagttt tagactggat attaatattt ggattcaatt
     4801 tgggaataca aggagctgca attgctacag taacttctca ggctcttaca gctattttaa
     4861 ctatagggta ttatataagt ggaaaatcaa acttacgatt cagtaaatct aatctaaaat
     4921 tagataaaaa gctaataaaa gcagtgtttg caataggtat gtcgccattt gctatgcaac
     4981 ttgcagcaag tttagttcaa gtaatatcaa atattgcact taaaactcat ggtggagatt
     5041 tagctatagg ggctatggct actatttctt ctattgcaat ggtatttttg atgcctatct
     5101 ttggaataaa tcaaggagcg caacctatta taggtttcaa ttatggagct gaaaaatatg
     5161 atagggttaa aaaagcatat ttaggttcat tagtagtagc tactataatt ttatgtatgg
     5221 gtatggtagt agttatgctt ttcccagaag ctattatagg tatatttaat aaagaccctg
     5281 aacttatgaa tatatctgtt aatggattaa gaatatactt acttatgttg ccaattgtag
     5341 gcttatcagt tacaggaaca aactttatac aatctatagg aaaagcaaaa atggctatgt
     5401 tattgagcct tttaagacaa gtaattttat tgataccagc agtattaatt cttcctacat
     5461 tcttaggttt acagggtgta tggacagctc aaccagtgtc tgattttata gctacagtaa
     5521 ttacaggtat agttgtattt agagaattaa aaagatatac tcctaaaact gaaaaattaa
     5581 atgaaaatga aagactaaac gagataacta ctgagtaaaa atataaattc agataattga
     5641 gcaagaaatt gcaaaaaata cgattatatg ataaaataaa accgctgtca aggataagag
     5701 aaattctaat tatggagtga ttttatggaa tttacaaaga aaagcaaatt aaaaaatatg
     5761 tatgtaaaca tgaatgtagc tagaatgttg tctaagataa atgagtataa gggtagacaa
     5821 ctgctttaca aaaaacaacc aaaagaaatt ttagaaaact tagaaaaaaa atctttagta
     5881 gattgttctg aatctacata cattggaaat caaagagata gtagtaattt taatctagaa
     5941 aagttgataa gtaatgaagt tacacctaga agtagagagg aattgagtat tgttgaatac
     6001 agagatgtgg taaaaactat taacagtgct tatgagagta ttcccatcag ttcacaaacg
     6061 attcttgagc ttcatggata cttatataaa ttttcatcta ctagaggagg aagttataag
     6121 tcagataatg attttattga acacaattta aaacctagcg aatttataag tattgattca
     6181 agtgtcaata aagttgaaaa agctgtagaa gaaatatgtg aagcttataa tacattgata
     6241 gaggaagatg aaattgatat attaatatta atttctgcat ttgtgttaga tttcatattg
     6301 atacatccat tcaaagaagg taatataaaa atggcgagag tacttatcct attgttacta
     6361 aataaaaatg ggtatgaagt tggaagatat ataagtttag gaaagatatt tgatgatagt
     6421 tcatatgaat attatagtaa tttaaattat ttgaaagcct caataggtag tgaaaaagca
     6481 gatatgaatg catggattga gtatttttta gagacgatat taactgcata tgagaagctg
     6541 gatgatagtt taaatatatc tgataagaaa agacaaacaa aaactagtag aatagagaaa
     6601 ataataaatt ctacccttgg atattttaca aaagaagata taagagattt atgtcctgac
     6661 attccagaac ctactataaa tagagttttt aataatctaa gaaaacaaga taagatagaa
     6721 gttgttgcaa gaggaagaag tgcaaaatgg aagaaaaaat attaaaaaat gtttagataa
     6781 ctaaaatggt atagtgtttt acactatacc atttttaatg tcattagcat gagcaataat
     6841 ttaacaactt aagcaatatc aattttattt attgattatt tttgaactga aaaatggcgt
     6901 tatatggtat acctcagtgg taattaattt tggatgagaa gatataatat tacagaatat
     6961 attaggagta tatgtagaaa aaatataata aaagtgtgag gcttagtaat taacaaatta
     7021 tgtggcttca cactttttta taaaataaag atacttagca gaagaatatt ttagttgata
     7081 tgtttataag atggtacttg tttgggtaaa agtataaata gtaaaaaagt agaagtaatg
     7141 tgtataaaaa cgtaagttac attctgtaat aatagttatt ttataattca acaaaagaat
     7201 agaatgggta tatttgtgaa ataataatat aaaataatct taggagtgag cttatgaaga
     7261 aaaaattatt atatataaat gtaaattcaa aacctgaaga cttatcttca agtaaaactg
     7321 tagcaagaaa atttataaat aaatttatgg aaaaaaataa agattttgag gttgaagaga
     7381 tagaccttta taaagaacat ataccaagac ttgagtatca gtactttgaa aaaagaaata
     7441 gcatagttag tgaagaaaat gctaaaaatt tagacgataa ggatagaaaa gaacttacaa
     7501 agattagaaa tctttgtgac caatttgtga gtgcaagtgt atatgtaata gcagcaccta
     7561 tgtggagttt atcttttcca gcaccactta aagagtatat agattgtata attcaagatg
     7621 gaaaaactat atcttttgaa ggcaatgata aaccacaagg aattttaaat gatattgata
     7681 gaagtatggt atatatacaa tcttctggtg gacacatacc atgggtgttg aagccagtta
     7741 tgaataaagg gttaaattat gtggaaagta ttatgaaatt tataggaatt aaaaaatttg
     7801 atgaattgtt agtagatgga actggaacta gtgaagaaga aagacaagct gctatagaaa
     7861 aagcagctga aaaaatagat ggtataatag atgggatgaa attttaatta taatatttta
     7921 ttaaaaatat aaaggaaaaa cctcttttaa tgtagaaact tatattgaag gaggcgagaa
     7981 tcatgcgaga agaaaaaagt aatgaaaagt atgattgtta ttggtgtaat caagagaata
     8041 atttctgtgt agaaataaaa gataatatag tcatgataga tgatggcact ggtacgttaa
     8101 aacaagccgt tttcataggg tataaacaaa tccaaattaa tttaaactgt tcacattgtc
     8161 aaaacttgaa tagaataaaa ttaaatttgt agaattttaa aatggaaatt attgaaggaa
     8221 ttaaaaatag aaagtgttaa tatttagact gaaaattata ttttatacaa taaaagttta
     8281 tttaaataac attgtatttt aatatttatt ttcagtctta ctttattaaa attatttttt
     8341 ggttatgtct ggaaaatatc tgtctactaa tttttctgaa atatttttta attggtcagc
     8401 cattgaagtc agacttaata aggctatcaa taaatcatct ttattttctg gatttatact
     8461 aatattttca aaattagact ctataaagtc cggtatattc tttctttgat tttcttttat
     8521 taacatgaat ttttcgtaac atgaaactaa atctttttca ctgaaattat ccttttcata
     8581 cacaccatgt aagattcttt taatttcttg tattgtaagt gtatttttca tagaataaat
     8641 aattatcatc tgtaaaattt gttctttagt atactttttt cccttaactg gtttgattaa
     8701 tccctctttg ctgtaattat ttatcatggt tttagtaaga attttatcac tttcaaatct
     8761 tttattggca gaaagcttat tgtcaattag agaaataatt tgatccatat ataagtcaat
     8821 agatggaata tcgtttgaag ttatatctgc attatttagt gtttctagta ttatagtatt
     8881 aagttcatta ttcatcatat gttacctcca taatatatat aatacactaa attagaaagt
     8941 aaaacaatat taaggcttgt aaaaaaaaca taaagtggta taattaaata tataatatag
     9001 taatggaaac tatatttgtg aatctgattt ttaaaataca taattgtagg aggacgaaat
     9061 gtatcaattt tttttaaagg gtagagaccc aataagcagt ttaactcatt ttataggtgc
     9121 ttgtttatct cttttagcaa ctataatttt agtttttcaa tctgttacat tacaggaaac
     9181 ttctctcttg atgatagtat ctgtatcggt atttggattt tcactaattg ctttatatag
     9241 cgcaagttcg tattatcatt ttttaaaggg gacaccagaa caagaattgt tttttagaaa
     9301 agtagaccat gcaatgattt acgtattgat tgctggctct tatacaccaa tatgtctgaa
     9361 ttttatggaa aaaaaagaag gcataatatt tgttacagca atatggatag ttgcatttat
     9421 tggtataata ataaaaatat tttggatgga tgcacctaga tggttatcta ccagtatata
     9481 tttacttatg gggtgggcaa ttgtttttga tataaatgca ttcaattcaa ttcctaagga
     9541 ttgccttaga ctattaatta tggaaggtgt atcttactct ataggtgcaa ttatttatat
     9601 aattaaaaag cctaatataa gtcctgagtt tggatttcat gaaatatttc atatttttat
     9661 tatgataggt tctttatttc actttttagc agttttattg tatgttttat agttttaaaa
     9721 gatttgatta attgatacta aaattctaca atgaataaaa tggaactgcc ttatatagat
     9781 tatatcttcg acaataagac agttccttat tataaatatt aaattctact tataataaaa
     9841 ttttgataaa aatagtccat tcaaaatgcc tattccataa ttcctttaat gtacttttca
     9901 tttacgacat atagtttgaa tttatgtact ataattatat cctctaaatt ctctgaaaca
     9961 ctaacaattc tgacacattt gtttctaatt tctacaggta aatctaaatc atttgttaaa
    10021 ttcacatagt catcatattt atacataaaa tcatcccttt acattaagat atacttatta
    10081 tactatattt taataagttt ttttaaatta ttacaatttt tcccgaaatt tcatttttta
    10141 tacataaaat ttccaaatta ggtaaagatt aaatttttta ttgta
//
LOCUS       DAYASV010000135        10104 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYASV010000135 DAYASV010000000
VERSION     DAYASV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.138"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            167..1066
                     /locus_tag="ACGI68_003130"
     CDS             167..1066
                     /locus_tag="ACGI68_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0139551.1"
                     /translation="MSIVLNMPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKN
                     NKINFEAVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLV
                     GNYIAERYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVN
                     QKLSEWTSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLF
                     RYGGDEFLFLLQNTSCVEAEKIMININYELSKLSNTDEYPYALSISYGLVEWDGISDI
                     ETLISKADSLMYENKLKNKSTLN"
     gene            1275..1424
                     /locus_tag="ACGI68_003131"
     CDS             1275..1424
                     /locus_tag="ACGI68_003131"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0139552.1"
                     /translation="MTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIKDFEKLMF
                     QNSSN"
     gene            complement(1684..4251)
                     /locus_tag="ACGI68_003132"
     CDS             complement(1684..4251)
                     /locus_tag="ACGI68_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HFH0139553.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYMSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(4254..4859)
                     /locus_tag="ACGI68_003133"
     CDS             complement(4254..4859)
                     /locus_tag="ACGI68_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139554.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLNGIMNDGGE"
     gene            complement(5233..6693)
                     /locus_tag="ACGI68_003134"
     CDS             complement(5233..6693)
                     /locus_tag="ACGI68_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HFH0139555.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRGNIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            7055..7633
                     /locus_tag="ACGI68_003135"
     CDS             7055..7633
                     /locus_tag="ACGI68_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139556.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKSLKMLYRLYDKN
                     PFLYSPNNPDFISLINKLPQDMFLQLESNNIQSFYDLIERHNLKLKIEKEKVFGVCNA
                     LLSSLLVKENIEYNYIEVFDFMVDNLVEHILE"
     gene            7924..9174
                     /locus_tag="ACGI68_003136"
     CDS             7924..9174
                     /locus_tag="ACGI68_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139557.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            9335..9904
                     /locus_tag="ACGI68_003137"
     CDS             9335..9904
                     /locus_tag="ACGI68_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0139558.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         3385 a         1734 c         1479 g         3506 t
ORIGIN      
        1 ttgtacttat ttaaagtttc atctaattat aaattggaca atttttatgt tgattttatt
       61 catacaaatt aaagtaactt ataaaatctc cacaataaaa aacatttttt attcactaaa
      121 ctgctccatc attggtttag caataaatat attttttagg tctttaatgt caatagtgtt
      181 aaatatgcca gcatatttat ttgatagtaa ttctattgca aatatttaca gagtatttcc
      241 tatatcattt agctttatag ttacaggact atatttcagt tataaaaata ataaaaataa
      301 taagattaac tttgaggcag ttttagagga caaaaaaaac ttaattttct tattgagcct
      361 tatgtttgga atgtacttgt atctatgttt gagtttgtca atatactata ctgttggaaa
      421 tgtattgatt ttaaaattat atggaatcaa gtctgccata atagttttgg taggtaacta
      481 tattgcagaa agatatagct ttagaattac tcaaataaaa ggttatgata ttaaaaatca
      541 aatagaatat gtagaaagtg taaagacaga atatgagatt aataaattga atgatattgt
      601 atatacagat ttactgacag gtttctacaa tagacctttt gtgaatcaaa aactatcaga
      661 atggacatca aatcactaca aatttacctt atgttttact gatttaaata gcttaaaacc
      721 tgtaaatgat aaatttggac accaatttgg tgataaatac atatccacta ctgctcaaat
      781 aataaaaaaa cttataaatg tagaaaattc cttactattt agatatggtg gagatgaatt
      841 tttattcctt ttacagaata caagttgtgt agaagcagaa aagataatga ttaatataaa
      901 ttatgaatta tcaaaattaa gtaatactga cgagtatcct tatgctcttt ccataagcta
      961 tggattagtt gagtgggatg gaatatccga tattgaaact ttaatatcaa aggcagattc
     1021 tttgatgtat gaaaataagc ttaaaaataa atctacttta aactagctag ttaagatatt
     1081 ctacaaaata tatgttataa gagagtacta agaatatcaa aatatataga ttttgcaaat
     1141 attgaataca gtttactaat gaatgcactg gatgtaagtg ttagtaaaca cctattagat
     1201 gaacacttta ctgttgtttg ggctaatgac cgttattatg aaatgttcag ctatacctag
     1261 ctaaaaagtt aaaaatgacc actgtagccg agggaataga gaccagtttt caaaaagatt
     1321 tcttgcaaga aataaactgt gatatgttac aaggatacgt gttctctaga cctttaccaa
     1381 taaaagactt tgaaaaactt atgtttcaaa actcctccaa ttaacaatat aaaattgctt
     1441 attataagtg atattattgt atcaacaaat aaaggaattg tctcaaaatt gattttttct
     1501 aatcaaattt ttattttgag acattctcat ttttttgcat aagaaaatac ccaccaagct
     1561 acggtttttc ttaccgctgt tcggtgggta ttagtagtat tatgaaatct gaaaacttta
     1621 agtattacta cttccgctaa cgcaatcaca aaggtcgaaa gcttcaaagc gaaaataggc
     1681 tttttactca tcaaacgtca ccgtacctgc caacccgtcc accgttatca tttgtccgct
     1741 cttaatagtt cgtgtggcaa tgtgtgtagc cataacggct gggatgccat attcacgtgc
     1801 gacaatgctt gaatggctaa gaggaccacc aatgtccgtt acaatcgcac ttgcagatga
     1861 aaataatggt gtccatgctg gagttgtggt aactgcaacc aaaacacttc caggttgaaa
     1921 gctttcaaaa tctgctggac tattcagtac acaagctgga gccgtcacaa caccagtact
     1981 ggtaccaatg cccgtcaaca caattttccc atctttctgc ttctgttgtg cttgtgatat
     2041 agtttttttg tttttctctg gcaacttgga tggtgacata taacccatgt attttttaag
     2101 ttcagctttt cgaacaggta ttatcccgtt catatcagat agagacttgt ttttatccaa
     2161 ctgctctata agtacctcca attccgattt tgtgagccaa taaatatcat ctaaatgtga
     2221 aatagctcct cctcgtatga atcgttctga gatttcctga aacatacaac gaatcagagg
     2281 atgtcccatt cccatcaaat aaatagcatt ttcacgcata ggagaggttt cctgtgccca
     2341 atggagcaac ttaaaaaata gttttttacg caatccacct atgtgttgta aaatctcttc
     2401 ttctgcatgt tttctacgct tttcaaacgt ggtctggcgt aaaaaaggac tttccccttt
     2461 tccttccaca aatgttttta cggattcaaa agttggtgta agtgtttcct gcggtgtgga
     2521 atgcgcaaaa tcaaactcat aagcggtacg accaaactct ttaaagtact tattgattcg
     2581 atttttccat tctatccata cttcctgtga aactccaaca ggcacaactg aagacatgaa
     2641 atcttctgct atctttattg caggactgtt ttgcagataa aggttgatgc tgttattttg
     2701 ctttacccat tctgaaatac cccacaactc cttttccgat tgcagaacaa tcgtatcaaa
     2761 cccaagcagg aaaacggaag tatccgttat accagcacgt cgaactgctc cttgaaataa
     2821 ttttgtaaat aatgtttcac tgatagaagc agcaggcaat gtaagctgaa tccttgtgaa
     2881 ataaatacat gcggcataaa atattgtctg aataccctcc ataatttgat gggcgttcat
     2941 agtatgcagg ggcttttctt cccattgttt aatagcatcc tcatatcctt ttcgtgccgt
     3001 ttcccagcgt gcaacgctgt tcataagtgt cctacgtaaa gagcgtgggg aaagggattt
     3061 gatagcaatc aaaagtggct ttgatttagc agaaagataa acatatccat taataaccgt
     3121 atatgccccg ttttttggta ataatttttt cgcactttta ccaaacatat ctatccataa
     3181 aagctctgat gcacggttta cgatttcaag accaagtgtg gcgaacaaag gtgtaacagg
     3241 atttggcaaa tgttctgcca aacttccctt tgtgtataca acatcctgtt ctggcaatac
     3301 ccattctggt ggcagaacag taattggacg agcctgaaca atgtacaatt tatctttttc
     3361 cagtgcccac tctacatcca taggcatttg ataatacttc tcaattttct ttccaagctg
     3421 tatcagttgc attacctgat tacgagtaag agcatatttt tttctcaacc tttcaggaac
     3481 catggtttct tctgttcctt ctgaagtacg aaccgtcata atctctttat ttgccacttc
     3541 atacgatata attcgttcgg agtccttatc cactacgatt gtatcgggtg taactaagct
     3601 agaaaccacc gcttcaccaa gcccccatgc agcgttaata atcatttcgc tacgtctacc
     3661 attgattggg tttaacgtaa acataatacc agatgaatcg gaaaatgcaa gcttttgtac
     3721 tacaacagca agtgcgacaa tctcgtgctt tatattattc ttcacacgat aagcaatagc
     3781 acgagctgtc cataaggaag cccagcatct ctttacagag tcaataactt tatcttcacc
     3841 ttgtatgtta agataagttt cctgctgacc tgcaaaagaa gcgtcaggta aatcttcagc
     3901 agtcgcagac gaacggacag ccaccgatat gttccctaat ccagcataag ccattttaat
     3961 tgcgtccgac acttcctgtg gcatttctcc attatggaaa agctccccta ttttcttaga
     4021 tacatcttca agttggctgg tattattgga atcaatacca tctagcagtt tattaattcg
     4081 agactgaata tggttcgtct caacaaaaat ctggtatgaa gcagtcgtaa catgaaaacc
     4141 gtctggcaca gggatgcctg ctgtcaatag ttttgataaa gacatccctt ttccaccaac
     4201 aatttcaatg gttgcttgtt tatgttccag tggcaagaca taataaatca taatcattca
     4261 cctccatcat tcataattcc gttcagaaat atattcattg tttcatccaa cattttttcc
     4321 gaactatcca atggtcgtga cgtgtagctg attgataaca tggagttaat taacaaacgt
     4381 gtcacaagag cggtatccgt tttgcgaaag atgcctgtgg tcacaccatt atcaattacc
     4441 gattttataa tatcttgata tgcataacga gcatctttaa gtctctttcg atactcttct
     4501 tctaaataat cagattctgt atttaaccac atcagtacag ttctatactg ctctggaaat
     4561 ctaagatgat taagcatgat ttttctaagg cactgttgtg gtgaaggttc accatcaata
     4621 atcctatgaa cttgttggat agttttttct atttctttct caactgcata tacaacaatc
     4681 tcgtctttcg ttttaaaaaa atcgtataga cttgactttc ccatgttggc taaaactgca
     4741 atttctctca ttgatgtttt ttggaatcca ttttcagcca tgagactcaa agcaacagca
     4801 actatttttt ctcggcgttc tgcttgttgt tcgggtgtaa gcacaattcc ttttggcata
     4861 gtgcgatacc tccttttagc gaccactggt cgtttctttt ttatttatca tacagcaacc
     4921 aacggtcact tgtcaaggtt aatttttttc attgtatatg gtactcgaga taaaaatgtt
     4981 cattttagaa attactctat ttaagtagaa ataggcttca tcattagaaa gcaagactat
     5041 aaatttgaga tatttatcat gttcaaattt catcaatatc aaagtttaaa atattaataa
     5101 aaaaagaatt actaagaaat aacatataaa gattcaaaaa aggagttgaa cttaaaattc
     5161 aattacaaaa aatcaaattt aagacaactc ctttattctt tatctctact attctttatc
     5221 tctatttatt tattacaact ccctaacatt taccactact gccttaggtc tagtcatagc
     5281 ctcaattgaa tacttaacac cttgagttcc cataccagat gcctttacac ctaaaaatgg
     5341 gaagtggtct ggacctcttt cagtcttatt atttatttga acagtaccaa cttcaagttt
     5401 atcagctata taaaatgcct tatcaatatc acttgtaaat actgatgatt gaagcccata
     5461 ctctgattga ttagcaattt caattgcttg atttatatct ttaactctta taattggaag
     5521 tactggacca aatggttctt cccaagcaat tttcatatca acatttacat tatctaacaa
     5581 tgttggatag aataaattat tttttctaac attgcctgtt attaactttg ctcctttatg
     5641 taatgcatca tcaaccaacc cttgaacaaa atcagttgct ttattatcta taagaggagt
     5701 gataactact tcatccattg gatttccaat tgtcaacttc tctaccaatg gctttatttt
     5761 tccgactaat ttatccgcca cactctcttg tactaaaatt ctctttactg ccgtacatct
     5821 ttgtcctgaa tatgaatacg ctccagatac tatattctta gctgcaaaat ctaagtctgc
     5881 atcttctaat actatagccg catcttttcc accaagctcc aataataatg gtaccattcc
     5941 agatatttta gatatatgct gaccaacttc agtactacca gtaaagttaa taaaattaat
     6001 tcctttatgc gttacaacat aatcccctat ctcactaccc ctacctgtaa tagtattaag
     6061 tactccatct ggtatcccag cttttctcat aatttctacc acatgaagtg cgctaatagc
     6121 cccttgtgta gcaggtttta atacgactgc atttccacca ataagtgctg gagcaatctt
     6181 tgacattgat aaatttacag gataattaaa aggagatata gctaaaacag tacctaatgg
     6241 aacccttgaa acataagaca tcttatttct tgttccacca gggaaattat ctccactaat
     6301 ggcttctccc tccatatttt ttcccacatc agctgtgtat cttaaaaaat ctgctgttct
     6361 ctcaacttct gcccttgcag attttatgtc tttggctatt tcaagtacca gtatattagc
     6421 tatctcatct atattttcta acaataaatc agcagcctta tgaaatatat tgcctctctt
     6481 gtatacaggc atttcagccc atacttttat actctctttt gtgtttttta taacctcatc
     6541 tacctcatgt tttgtcatag attgtacttt cccaacaaga gaattatcta ttggtgagta
     6601 aatttctatt ggctttctcg aattagattc tacccatttt ccatttatta aatttttata
     6661 tacattttct tttgttttaa gttcattaaa catattttta ttcctctctc tgatataact
     6721 ttgtgtattt aaattatagc catttattcg ttatgtaaac atttcttttt ttcataaacg
     6781 tttacctata aaaaaatttt aagttaacgt ttttctacat tcttctattt taataacata
     6841 ctcgctccaa atctatatag gtcacacttt aatactaata tatcaatata ccaatatacc
     6901 aatataccaa tataccaata taccaatata ccaatataaa tattataaac ataaatcact
     6961 tgacaaattt ataagtagac aatattatat atctatgact caaaatattt ttaaagtcat
     7021 atatatcata tatagacaga aaggattgat aaaaatggct aaaagtttta cagaaataga
     7081 aaaagacaat ataagaaaaa gactaatttc agaatgtgaa atcaactggt ctaaatatgg
     7141 atacaaaaaa actaatattg atgaactttg ttcaaaatca ggaattgcaa agggttcttt
     7201 ctatacattt tttgattcaa aagaaaagtt attttttgaa gtattaaatg gaatccaaaa
     7261 cagacttata tcatcagtag atgaaatttt aagtaacatg cctccaaaag aaggctttgc
     7321 aaaatcctta aaaatgttat atcgattata tgataaaaat ccatttttat attcaccaaa
     7381 taatcctgat ttcatcagcc taataaataa actacctcaa gatatgtttt tacaacttga
     7441 gtccaataac atacaaagtt tttacgactt aattgagaga cacaatctca agttgaagat
     7501 agaaaaagaa aaagtttttg gtgtttgtaa tgctctacta tcaagtctgt tggtaaaaga
     7561 aaatattgaa tataactata ttgaagtgtt tgactttatg gtggataatc ttgtagaaca
     7621 catacttgaa taacaattta aaataatttt tctaagtatt tttttatact aaataggatt
     7681 aatatgattg aattaatgca aaaaataaac aatataattt ataaaataaa tgataaacta
     7741 aatatcacac aaaacttata gaccctagag caaacccatt ccactctact ctaagccatc
     7801 tataatagtt tgtatatttc taataatatt agatattaaa gtaagaaaaa aattccttat
     7861 aataattgct attatatcaa ttattgtaaa tgttttaata atcttagata ggtaggtaca
     7921 tatatggtaa aatcctcgaa cacttttaat aaaaacttca acttgatgat attaggtcaa
     7981 ataatctcat tgtttggtgc atcaatactt aaatttgctt tatctttata catacttgat
     8041 ataactggta aagcagaaat atttgctact atactagcag tatcttcaat cccaattata
     8101 gtattctccc ctattggtgg agctattgca gatatattca acagaagaaa cttaatggtt
     8161 atttttgatt tttcaagtag tcttacagta ctaatcttag cagtattctt attcaacaat
     8221 aatggttcaa tattacttgt aggaattatt atgaccatat tatcagtcat aagcacaatg
     8281 tatcaaccaa ctgttcagtc aagtacaccc cttttagttg ataatgagca tttaatgaat
     8341 gcaaatggta ttgtagctgg agttgcatct cttacaaata ttgcaggacc tgtattagga
     8401 ggtgtcttgt atggtgtgat tggaattaat gcaattatag ttataagttg tgtaagtttc
     8461 tttctatctg caattatgga gatatttata cagattcctt ttatcaaaca agagcaaaac
     8521 agcaacatta taaaaactat atttactgat ataaaggatg gtattgttca tatatgtaaa
     8581 gaaaatagat ttatactaaa aatacttttt ttagctatag caataaactt atttttaagc
     8641 tctatgttaa tagttggtat cccctatata gtaaaaatca ctatgggagc tagtaacttc
     8701 ttatatggtc tagcagaagg agcaatttct tttagttcac tggtagctgc tgttagtatt
     8761 ggtattttct caaaacatct aaaaatcact aatttgcata tttcctttat tatatgtgca
     8821 gttatgttca taccaatggc ggtttcagta tatccattta tattaaacac aggatttcca
     8881 gttccatttt taatctttat agtatcatct atgctaatat tttttacaac gactcttatc
     8941 tcaatatatg ttataacagt cacacaatta aaaactccaa atgaactctt aggaaaagta
     9001 atgtcaattc tttttgcagg aacagcaatt gcaatgcccc ttggtcaggt tatgtatggt
     9061 aaactttttg atttattatc agatagagtg tacatcatta tactaggagt tggatttatt
     9121 acattactta tagcatttgt atcaaagata acattgagga acgaaaatct ttagatacaa
     9181 agaatttaga tataaagatt taattttcac taatttctga atttaaatgc aaaattgtgt
     9241 atcagtatgt tgacgtttta gaaattgatt gatataatca atggaaaata aactttataa
     9301 aaattcaaaa tattcagcta gaggagtgat aaaaatgagt tacatgccaa ctttagaaca
     9361 agcatgggaa atattaagaa aatacaataa ggaagaattt catataagac atgctcaaat
     9421 tgtatctgga gttatgagat actatgcaaa ggagtacgac cctgaaagag tagaattttg
     9481 ggaaatagtt ggacttttac atgatttaga ttttgaaatg tatcctgatg aacattgtgt
     9541 taaacaaaga gaattaatga cagagcttga tttagataaa tcaataatcg attctacaat
     9601 aagtcatgga tatggattaa ctggttcaga tgttaaacca gagcaattta tggagaaagt
     9661 attatttgct gtggatgaac ttactggttt aattggtgct gctgcaataa tgcgtccttc
     9721 aaagagcgtt tctgatttag aattaaaatc tgttaagaag aaatttaaag ataaatcatt
     9781 tgctgctggc tgttctcgag acgttataag aagtggtgca gaaatgcttg gatgggaact
     9841 tgataagctt atccaaacaa ctatagaagc tatgagaagc ttgattccag agatggcaat
     9901 atagctcctc catacatata ccatagaagt tacatcttta aagagcattt ctctttttga
     9961 tgcaacttct ttttatattt tgtagaataa aaagtttaat attttaaact cactatataa
    10021 taaataatat aatgtataat agtcataaac tttctaacct taaaagtttc taataatcaa
    10081 taaacaattc taatatacaa atgt
//
LOCUS       DAYASV010000136         9877 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYASV010000136 DAYASV010000000
VERSION     DAYASV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.140"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            1117..2475
                     /locus_tag="ACGI68_003138"
     CDS             1117..2475
                     /locus_tag="ACGI68_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0139559.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2582..3376
                     /locus_tag="ACGI68_003139"
     CDS             2582..3376
                     /locus_tag="ACGI68_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0139560.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3369..5675
                     /locus_tag="ACGI68_003140"
     CDS             3369..5675
                     /locus_tag="ACGI68_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0139561.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKILSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5814..7091
                     /locus_tag="ACGI68_003141"
     CDS             5814..7091
                     /locus_tag="ACGI68_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0139562.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASFNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7274..7996
                     /gene="yqeC"
                     /locus_tag="ACGI68_003142"
     CDS             7274..7996
                     /gene="yqeC"
                     /locus_tag="ACGI68_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFH0139563.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRNKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHRTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIENI
                     KEYESEIEIFYGSVKQKFCVRY"
     gene            8103..8672
                     /gene="mocA"
                     /locus_tag="ACGI68_003143"
     CDS             8103..8672
                     /gene="mocA"
                     /locus_tag="ACGI68_003143"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFH0139564.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            8669..9688
                     /locus_tag="ACGI68_003144"
     CDS             8669..9688
                     /locus_tag="ACGI68_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HFH0139565.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT         3917 a         1021 c         1826 g         3113 t
ORIGIN      
        1 ttatcaatga gaacataaaa attttatact atacaaatgt atgcaaatat tggcactaaa
       61 attgctctat aaaaattata aatagtaata tgaataaaat ttttactata aaaataatat
      121 tactattttg cttatagata tatatatcta taaatagata gaaacttgta tattttatgt
      181 tgcagataca aatatagtaa aggagagtac attggtacaa ctgagataca tagcttatta
      241 agtttttctg tcttatgaga attatattat aaaatattca taaatatttt gagtttaaat
      301 taaataatta ttttatgctt agattaattg atatttaatt aatttaataa caaaaacagt
      361 gaaagagtaa ataatattat ttacaggggg taaagcaaag tatgttaaat aaattgtact
      421 tttggccccg tacatagaga aatctatgta ggacacaact ttaaaaccag taaatcctaa
      481 agccttgcaa ctacaataaa agatagaaag aaacttttat gaatgtggtg aaaaccagaa
      541 acaatagtga ggatggtata tggtgagagc ctaagtactt aaaaatggac atttggtcgc
      601 caagcctaga aataggaagg tcaaacgact atctcgagag agagtacact ataagcgatt
      661 gatagtggaa acgggttgct cttagcaggt aatgctgaag atgaagaaat agtctacgct
      721 catatgaaag tatgaggtca ctggatttga agtgacatct ataaagtagc gtttataggt
      781 aaatgtttaa aacttgaaac atgtaaatac ttatgaaaac ttataactta taatgattaa
      841 gattgaaaga tttaaattga attaaattta ctataaaaaa ttaaaaattc atagctataa
      901 tactcttata tttgtaaagc atggtatata atgtatacaa aattaaagaa tacgtgtatt
      961 tgttaataat taaatttatt gcatctgttt tttaagacta aaaacttaaa tatatgttgg
     1021 agtatagttg atatttatga acatcattaa tttaatatat tagattttta agtatttata
     1081 taattcaata agttttatgt aacgggagtt tttattatga aagcaaaaag tacatcaaaa
     1141 tttgaattag acggaatacc accactaaag gaagcaatac ctttagggtt acaacactta
     1201 atggcaatgg taataggtag ttgtttacca gcgatattga ttgcaaatgc agctggactt
     1261 aatcataaga tgtcaacatt attgattcaa gccgcattag tttttgcagg attatcaact
     1321 ttattacaat tatatccaat acctttgttt aaaggtttta aattaggagg aggaatacca
     1381 gttataatgg gagctacagc tatgtttata ggagctgggg tatctgttgc aagtaagtat
     1441 ggactaccag tattatttgg ctctcaaata atagctggta tagtaataat tataattggt
     1501 tttggtctta aaaaaatacg atttatattt actccagctg tatcaggaac aatagttgca
     1561 tgtatgggtc ttggactttt tccaatagct ataaaaaact tagctggggg tatgggcaat
     1621 gagacttttg ggaatccaat taattttata gttggaatat ctgtatctct tatgatatta
     1681 ggattaaata agtatggaaa aggattattt aaagatgcat caatattagt tagcataatt
     1741 tttggataca ttttatcatt aattttgggt atagtaaatt tttcttcaat tcaggagttt
     1801 actttggttg cacttccaaa accacttgct tttgggctag atataagact tgaagttgtt
     1861 gtaatgtttt caataatata tttagttgag atagctgata taatgggtgc ttgtacactt
     1921 tctgcggtag gagggcttaa tagacaagta acagatgaag aactttcttc tgctgtttta
     1981 ggaaatggag tactctctac aataggtgca ttattttctg caactccaat ggggatgttt
     2041 ggacaaaatt ctgcaatcgt ttccaataca aaggttgtaa gtaaatttgt actagctatt
     2101 ggaggaattg gattattttt ggcaggaata tcgcctttat tagctaattt aataagaaca
     2161 attcctccct gtgtggttgg aggagcaaca ctcgttatat tttcaactct tacaacatct
     2221 ggtttaagac ttgttagtat ggatggattt aatcaagaaa atagcatgat attaggtctt
     2281 tctatggctt ctggtattgg attcatggta gctcctcaag ttcttgaaaa atttccaaaa
     2341 tttatagaga cattacttgc agattcaagt gtagtttcag gagcaatggt agccataata
     2401 attcaagctt tatatatggt aaaatttcct ggaaataaaa gtgataaggt tgaggataaa
     2461 aataaaagtt tataatgtaa aggtttaata tatgtgaatt aaatatacta caattataaa
     2521 tataaataaa agatttatta aaaacttttg tgttttaata agttaatgat ggggggataa
     2581 gatggttact ataaaggaat attcagttcc acagagttta gaagaagcat ataaaatttt
     2641 aatttctaaa aaaaataatg ttattttagg tggatgtgga tttataaaat taagtaataa
     2701 aaatatagga acagcgatag atttaaaaga tataaatcta aattatataa aagaggataa
     2761 aaaaaatatt ttgataggtg cagatacttc acttagaagt ttagaaattg ataaaactat
     2821 aaaaaattac tgtagtggaa tactttcaag cgctgtttct aatatagttg gtgttcaatt
     2881 tagacaaggg gctagaattg gtgcaagtgt attttcaaag tatggctttt ctgacttaat
     2941 accagcactt ttggttgttg gagcgaaggt gagattatac aataaaggaa tactagagtt
     3001 aagtgagttt ttggatagtg aattaagtag ggatatattg gtcgaagtaa tactaccaaa
     3061 aaaagatgca atagctgttt ttgactcaat aagaaaatgc acaggagatt tttctgtatt
     3121 aaattcagca atgctaaaag aaaatgatac ttacaaaata gcaattgggg caagacctca
     3181 aaaagctaga ttagcacttg aagcgagtaa tatattaaat aaagaaaaag atatagataa
     3241 ggcatctatt gttgctagta aggaacttac ttatggaagt aatatgagag ctagtaaaga
     3301 gtacagaaag gatatggcaa gtgcattagt taaaaggatg tataatgcta ttgaggaggg
     3361 aatgtataat gattaataag gggataagaa agatagatag tacagcaatt gtcacaggaa
     3421 aacctctata tactgaagac ctcattatgc ataaagatgt tttgacatta aaactactta
     3481 gaagtccaca tgctcatgct aagataaaga atatagatac atcaaaagcc gaaaaaattc
     3541 caggggttgt gggtatttat acgtataaag atgtaccaca ggtcagatat tgtgttccag
     3601 gtcaaacaga accagaagca tcaccttatg atagagtttt acttgaagat acagttagat
     3661 ttattggaga tgaagtagct ttagtggctg ctgaagatga gaaaacagca cttaaagcct
     3721 taaaaatgat aaaggtagat tatgaggttt taaagcctgt gtttgatgca agagaagcag
     3781 aaggaaatga agttgttata catccagaaa aagacatagt agagccatac ccttttggat
     3841 tggatgcaaa aaataatata gtatcaaaag aattttttga atttggagat gttgaagcta
     3901 agcttaaaga aagtgatttt gtggttgaat cttcatacaa aacccaaagt caatcgcatt
     3961 gcatgatgga aactcatagg tcatattcat ataaagatat aaatgataga attgttgtag
     4021 tttcatcatt acaatcacca cacaatgtaa agagaatact tagaaaagta ttgggtatac
     4081 cgtcttcaaa aataagagta attaaaccta gaataggtgg tggatttgga ggaaagaata
     4141 tagcagcaac agatatattt actgcatttg ttactatgaa aactggtaga ccagcaaaat
     4201 ttgtatttac aagaagtgaa acttttgtag gttctaatag tagacatgaa atgttttttg
     4261 atgttaaagt tggtgcagat aaagaaggaa atttaaaagc aatagagcta aagtcattaa
     4321 acaatacagg tgcttatggt gggaatggtg tctctgtttc ttccgaatct ggacacaata
     4381 ccttaccaat atataatgat attgatgctg ttagatttaa tgctagaaca gtatatacta
     4441 ataggttacc agcaggagca cttagagggt atggtgctac acagggaata tttgcaatag
     4501 atagtgctat tgatgaattg gcacataaga ttggaatgga cccacttgaa tttagaatta
     4561 aaaatataat agataggtat accaatggaa aagcagctga agacgaaata ttaagttgtg
     4621 agctgagaca atgtatagaa aagggaaaga aattaattaa ttgggatgat aaatatccat
     4681 gtaaagatat aggaaacaac aaagtaagag cagtaggtat ggcagtagca aatcatggtt
     4741 ctggtatacc gcgtgtagat atggcaacag tcactataag aatggatgaa gatggcacat
     4801 ataaaatatt atctggtctt gctgacttag gtcaaggggc tgatacaatt caagctcaaa
     4861 ttgctgcgga agctttaaat actactgtag atagaatttc tatttataca ggtgattcag
     4921 acttatgtcc ctatgataca ggagcatttg cttcttgtac gacaacggtt acaggaaatg
     4981 caacaataaa gacagcaaat aaattaaaag atatattact ttcagttgca gagtcaaagc
     5041 taggatttaa aaaagaagat ttagagttgg gaaaagacag ggtttttgtt aaaaacgata
     5101 atagcaagtt tgttctatta gaggaattgg gtagagaatc tgttggagga ataggatttg
     5161 ctagtgatga tgtaccagta gttttggaag caacagaatc ttttggcatg ttgaaaaaaa
     5221 taaaaccatt tatagcagga tttgcagaga tagaattaga taaaaataca ggtgaatata
     5281 aagtcataaa ttttgcatgt gtacctgatt gtggaacagt tataaatcca actttagcta
     5341 gaatacaagt agaaggtggt gtgatgatgg gtatagggtt tgcaatgtat gaagacccta
     5401 tatttgataa agatggtaga ttattaacag atagtttcct acagtataaa gttcctacaa
     5461 aaaaagaagt tggaaatata ttggtagatt ttgctgagaa tttcgaccca ggaggtcctt
     5521 atggtgctaa atcattagga gaaatagtta tacatacacc agcaccagct atagcaaatg
     5581 caatatttaa tgctacagga gttcgtttaa gagagcttcc tatgactttt gaaaaagttt
     5641 atatgggtat gaaaaataat aataaacaat tataataaat aataagatgc ataaaagcaa
     5701 gtaaatttaa attattttgt aaaataaagt aattaaatgg acattatata tggtgttttg
     5761 taaaaatacc gtatataatg tctagtagag tatattctaa aggaggtgag ttcatgtcaa
     5821 acagaataaa aaatatacac tatggaactc agagacaaca attaaaaaac ttaactaaaa
     5881 aagaatatca agctctacga gagatgtgct tcttagctaa gaacttatat aatgttggta
     5941 catataatgt aagacagcat tatttcaatc aaggagaaca tcttaactat gaagctaatt
     6001 atcatgtatg caaagaaaat gagaactata agcttctcaa tagtaatata gcgcaacaaa
     6061 tattaaaaga agtagacagt tcgtttaggt cattttttgg attaattaaa ttagccaaag
     6121 aaggaaaata tgactttaga gggataaaat cacctaaata tttagataaa gaaggatttt
     6181 ttagcataat aataggtcaa ataagaatca aagaagatgg gattttaaat gtacctatgt
     6241 ctccagcatt taaaaaacta tatgggaaag taagtataaa agttccacaa aatatactag
     6301 agaaaaaaat taaagaaata agaataatac ctaaacataa agctaggttc tttgaaattc
     6361 agtattgtta tgaaatacca aaatcagagg aagtttcaaa tcaaaaaaat gcactagcta
     6421 tagacttggg tttagaaaat ttatgtacat gtgttaccaa tttaggtgat agttttatca
     6481 tagatggaaa aaggctaaaa tctgtgaatc agtgggcaaa caaacagaat gataaaatag
     6541 aagccttaaa attaaaaaag aatataactg caataacaag aaaacaattt aaagtttgga
     6601 ataagagaaa taatcaagtt aaagactatg taaataaaac ctgtttttat ataattgatt
     6661 attgtgtaaa aaataatatt ggaaatttaa ttgtaggata taatgctaag ttagaaaaaa
     6721 gtgataatat ggagagaaaa atatatagaa attttataag aatcccattt ggagagataa
     6781 aaggaaaatt agaatatctg tgtaaaataa aaaatatcaa ttttattagg caagaagaaa
     6841 gctatactag taaatcagat ttttttgctg atgattttct acctgatatc aatgtggaca
     6901 atgttaaaaa atatgacttt aaaggggaaa gaataacacg tggacagtac aaatccagtg
     6961 ttggaatcat tataaatgca gatgtaaatg cgagttttaa tatactaaaa aaatcacaag
     7021 tagttaattt atctaattta cagaataatc ctcaaatatt aaagcaacca cagagagtaa
     7081 gaatatctta attatattta aaagataaac tgatagcgta agcttacagc ttagcggcga
     7141 attggatccg ccttgttgtt tacaacagaa tctatttact ttcgagttta tggaatttca
     7201 aaaatcactt ttactaaaaa ggcctaacta taataattaa gagattagaa aactggttat
     7261 aagaggtgaa agtgtggaac tatcaaatat aatagaaaag aatgaaataa taactgtagt
     7321 tggagcagga ggaaaaactt cgtttataaa ctattttgca aatttttata gaaataaatt
     7381 gaaagttctt cttacaacaa caactaaaat atatgtacca aacgattatg acaatataat
     7441 tattacaata gatggaactg ttattccatc tatttgtcat ggaataacag tctgtggcag
     7501 ttatataaat aatgaaaata aattagtaag tattgattcc tctattttgg atgaaattgt
     7561 agaccaattt gatttagttt taatagaggg agatggctca aagagaaaaa aacttaaagg
     7621 ttggaatgct aaagaaccag tagtatatca tagaactaca aaaaccatag ggatattaga
     7681 tataacatca tttggaatga atattaatga agagaatata catagagtag aaatatttaa
     7741 aaaaatagca aatttagata caagtagtat taatagtagt tctacagtaa gtattgaaaa
     7801 cttaaaaaat atagtattaa atccaaatgg attatttaaa aattattctg gaaaaagagt
     7861 attatttatt aataaagttg aaaatgaaaa atataaaaat ttagctataa aattaataga
     7921 aaatattaaa gaatatgaaa gtgaaataga gatattttat ggaagtgtaa aacaaaaatt
     7981 ttgtgtaaga tattgaagta ttaaaatagt ttatatgata ttatatatat agataatatc
     8041 aatagagata ttataaaaat catagaacta tcaatatatt ataaatacca aggatgatat
     8101 atatgataaa tgcagtgata atggcatcag gctttgccag aagaatggga gcaaataaac
     8161 ttttattaaa ttataaaggc tgttctataa tagaacatgt atttagagaa gttagcaaga
     8221 ttgattttca tcaagttgta gtagtaagtc aatatgaaga ggttttgtta ttgtgtaata
     8281 aatatggttt tgaatatata gataataaaa atgcaaatat aggtcaaagt gaatctataa
     8341 aattaggagt tttaaatagc tcaaaatgtg atggttatat gttctttgta ggagaccagc
     8401 catttattga taacttatat atagataaaa ttataaatac ttttaaatta gatagagatt
     8461 ttatagtaat tccaagatac aaagatatgt gtggaaatcc agtcatattc ccatatagca
     8521 agaaagaaga attgttggca cttaaagaag atgaaaaagg taaaaccatt ttaaaaaatt
     8581 catcaaaaat aaagtatgta gaagttccta aagaaatgtt atttgatatc gatacaaaaa
     8641 tggactatga gagaattggg ggtaaattat gaagctaata aaaactatag atgctgttgg
     8701 acaagtttta tgtcacgaca taactcaaat aatacctgga gaatttaaag gaagaaaatt
     8761 taaaaagggt cacatagtaa aagaagaaga tattccagtg ttattatctc ttggtaaaga
     8821 taatttgtat gtatgggaaa agtcagaagg aatggttcat gaaaatgaag gcgcattgtt
     8881 tttaaaagaa ctaactgctg gagaaaattt agattttagt gaaataaaag aagggaaaat
     8941 agattttata gcagcatgtg atggtctatt aaagatagat gttgatgcac tttttgattt
     9001 gaattgtgtt ggagaaataa tgatggctac tcttcataat aattttatag taaataaagg
     9061 tttaaaagta gctggaacaa gagtgatacc tttagttata gatgagaaaa aattagaaga
     9121 tgcaaaaaaa gtagttggta atagaaaaat agtaaatgta gtacctttta aacctaagaa
     9181 agtaggaatt gttactacag gaaatgaagt gttttatagc agaattgttg ataaatttgg
     9241 accagtaata gaagaaaaag taaaaggctt tggttgtgaa gttataggac agactatatg
     9301 cccagatgat aaagaaatta taaagagtgc aataaaagaa tttataagtc aaggagcaga
     9361 acttatctgt tgtactggtg ggatgagtgt agacccagat gatgttacgc caacagctat
     9421 aaaagaaact ggagctgatt tagtgacata tggttcacca atattaccag gagctatgtt
     9481 tttacttgct tattacggag aagttccaat tatgggtata ccaggatgcg caatgtatca
     9541 caaaactaca gtatttgata tagttttatc tagggtttta attgatgaaa agcttgataa
     9601 gtatgatata gctagatatg gtcatggtgg cctttgtatg aactgtgatg tatgtactta
     9661 tcctgcatgt aattttgcta aaatataaag ttattaaaaa agagtattta caaattaagt
     9721 actcttttta aatatgaaac aaaacagcta gaatgatgtt ttaaaaaaag catatttatt
     9781 tagaaattat aataacttat tttcaatgtg taaaagaatg aataaagttg caatttgatt
     9841 tctttataag tataatataa gagattgtta attttta
//
LOCUS       DAYASV010000137         9772 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYASV010000137 DAYASV010000000
VERSION     DAYASV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.141"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..766
                     /locus_tag="ACGI68_003145"
     CDS             <1..766
                     /locus_tag="ACGI68_003145"
                     /inference="COORDINATES: protein motif:HMM:NF040187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0139566.1"
                     /translation="YYIYKNKFMKVILMKTISKQELEKTLEKHYLWLKNNNAGEKADL
                     KELRLENMDLRGYCLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNA
                     DLSGASFRFCDLSNSDIRGANLENSNLEYATLNGVISDESTRFFKLYCPETGAFIGYK
                     KCFNFRMVQLLIPSDAKRVSATSNACRCDKAKVLSITSVDGKESFKSARAYADENFIY
                     RVGETIVAEDFNENRWKESTTGIHFFLTREEAIGYL"
     gene            complement(894..2090)
                     /locus_tag="ACGI68_003146"
     CDS             complement(894..2090)
                     /locus_tag="ACGI68_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139567.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFDLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(2093..3118)
                     /locus_tag="ACGI68_003147"
     CDS             complement(2093..3118)
                     /locus_tag="ACGI68_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0139568.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            complement(3190..4320)
                     /locus_tag="ACGI68_003148"
     CDS             complement(3190..4320)
                     /locus_tag="ACGI68_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0139569.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTQEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(4548..4729)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4782..5675)
                     /locus_tag="ACGI68_003149"
     CDS             complement(4782..5675)
                     /locus_tag="ACGI68_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFH0139570.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(5755..6657)
                     /locus_tag="ACGI68_003150"
     CDS             complement(5755..6657)
                     /locus_tag="ACGI68_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFH0139571.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(6707..7867)
                     /locus_tag="ACGI68_003151"
     CDS             complement(6707..7867)
                     /locus_tag="ACGI68_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0139572.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNLD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(8023..8328)
                     /locus_tag="ACGI68_003152"
     CDS             complement(8023..8328)
                     /locus_tag="ACGI68_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFH0139573.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAQGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(8426..9400)
                     /locus_tag="ACGI68_003153"
     CDS             complement(8426..9400)
                     /locus_tag="ACGI68_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HFH0139574.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
BASE COUNT         3184 a         1787 c         1070 g         3731 t
ORIGIN      
        1 ctactacata tataaaaata aatttatgaa ggtgatttta atgaaaacta tttctaaaca
       61 agaattagaa aaaacacttg agaaacatta tttatggctt aaaaacaata atgctggaga
      121 aaaggcagat ttaaaagaat taagattaga aaatatggac ttaagaggat actgcttaag
      181 taatattgac ttttcgtggt ctgaccttat caatttaaat ttagaaaaag cagatttaga
      241 aaactctatt tttaataatt cttatctttc ggattgttca ttaaaaaatt ctatattaaa
      301 aaatgcagac ttaagtggtg caagttttag attttgtgac ttatctaact cagatattag
      361 aggagctaat ttagaaaatt ccaatttaga atatgctact ttaaatggag taatatcaga
      421 tgaaagtact cgttttttta aattgtattg tcctgaaaca ggagcattta taggctataa
      481 aaaatgtttt aattttagaa tggtacaatt attaataccc agcgacgcaa aaagagtgtc
      541 tgctacatca aatgcttgta gatgtgataa ggcaaaagta ctgtcaatta ctagtgtaga
      601 tggtaaagaa tcttttaaaa gtgctcgtgc atatgctgat gaaaatttta tatatagagt
      661 aggagaaacg attgtagctg aagattttaa tgaaaataga tggaaagaat cgactacagg
      721 gattcacttt tttttaacta gagaggaagc tattggttat ctataaaatg ctaaagtttt
      781 ataaatatat aaatacataa acaaaataac aaaagagtat agattgtaat ttcaaaaaaa
      841 ttataatcta tactcttttt tctataacac ataagtacat tttatttcat aaattataat
      901 aaattattta tatctgcact attgattatt tgagaaactt tatctatatg ttctatctta
      961 ctatccaaac tgtcataata cttcttactc tcatctctat ttctaaaaga ataaactttt
     1021 ttatccaatt ttaaagcttc ttttataata tctatattcc tcttatttgt ctctcctaca
     1081 ctaaatcctg tatctattat aatcttggaa tcatttaaat ttctaatagc caaatcgaaa
     1141 ctctcatcac ttataggctc aaatggattt tctgtaaaca tctttatccc catagttcta
     1201 ccaatttcat aatcaatatc attttcatgt attattccag aatacagacc tatacctttt
     1261 ttagtaaaag ctctataaat tggtgttgct ttacccccac caccaattat aaaaacttca
     1321 tttttagatt tattactaat ttctactgaa cctaaaagac tattaaagtt tttatcatcc
     1381 aattcataaa gcgaatttat aatatcctca tctactacat cttcaggctg gccataagct
     1441 atgactttat tattttttat aagagcaact ttatcacaac tttttaatgc tatgtctatt
     1501 tcatgcaatg aaagtattac tgatattgac ttttctttac ttaatttttt cagtatgttt
     1561 atgagttcca atctatgttt tatatccaag tgagttgtag gctcatctaa tataattatt
     1621 tctggttctt gaacaagagc ccttgcaacc aacacctttt gtttttctcc atcacttagt
     1681 tcatcaaaat atcttttctt taaatgtaag gcatctacag actccaatgc ttcatctacc
     1741 attattaaat ctttcttaga tagactccca aaaaatcctg tatatggata ccttcctata
     1801 ttgactatat cttgtacagt cattaaatca cctaataatc tatttgtaag tactaaagcc
     1861 attttttttg ataatgcttt tcttgatata caatttatgt ttacaccatt caaatatatt
     1921 tcacctttta ttggtgatat aagtttagat aaggacctta atagggtagt ttttccagct
     1981 ccattacttc ccaaaagaca tagtatttct ccatttttta cttctacatt tatattactt
     2041 ataacaactt tgttattgta tcctacactt aaattattag ttttcaacat gtctaagccc
     2101 tatccttcct tttcaccaat aacattacta caattggagc tcctaataaa gatgttattg
     2161 tacttattgg taattctact ggtgataaaa ttactcttga tattaaatca caaagagctg
     2221 taactatact gcctaaaaat attactactg gtatcaaaac tctattatct gatgttttaa
     2281 atatcatttt tgaaatttgt ggcactgcaa gtccaataaa ccctataggt cctgcaaatg
     2341 ctgatactat tgctgctaat atactagata taaatactat caaaactcta aaaaatctta
     2401 tatttatgcc aatacttttt gcatactctt ctcctagtaa aaaagcattt aaaggtttta
     2461 ttatgaatac agtcatcaaa agacctaata taccaactat tataagtatt tctacctttt
     2521 cccatgtaaa tccagaaaaa cttcccatag accacatagt aaattctttt aatttatcac
     2581 tatttgcaaa tgattgtaac acatttgtta taccactaca tacatatccg gtcataattc
     2641 ctattattaa aagagttgtt atattcttta ccttatttgc aaataaaatg actatagaca
     2701 ttacaattaa agctcctata aaagctgcta caaatatact cattgaattt acactctcaa
     2761 atcccaatgt gtatcctccc aaaatcatga gagctactga taaagtagaa cctgaagata
     2821 ctcctaatat atacggttca actattgggt ttttaaaaaa tatctgtaat aaaatacctg
     2881 atactgctag gcatgctcca cctaccatgg ttgcaagtac tctagggatt cttatattgt
     2941 atattatttt ataatgttct gtattatcca tacctcttaa tacaacatct attacttctt
     3001 taaattcaat ttttacagag ccaaatgcta cacacactaa aaaagtaagt atcatactta
     3061 taattaatac tataaatatt gtcgtattcc ttattctcat tttattgata aaatccattt
     3121 gtatactcct tttagcaaag ctatattaac taaaatctat aaaacctatc tttttgtgtc
     3181 ctctctgtac tatttatcat aatttatata atgtttgatt ttttcacctg tagtacctgg
     3241 gtggaacatc tcaactaaat ctactattaa ttcgtctgtc ttatctactg attgataata
     3301 atctggagca aacacccata ggttcccgtt ttttacaaca tccaaatcag ccaatacagg
     3361 tgcactagat tttatagaat ctaaatttgg tgcataatct gaacttgatg agtatacaaa
     3421 tatatctgca tcttttgcaa cttcatgcaa ttgttcaata cttactcgcc cttcatttga
     3481 atctattgaa gcacatgcat ttattccacc agccattttt atcatattat ccacatatga
     3541 atctttagga gctatataag cacttccttc atatacagat gcccatacaa ctttaggttt
     3601 agctttgttt tttatttttt tacttacttc ttctaccttt tgtacagttt tatttagttc
     3661 actagtagcc atatcttctt tatcataaaa tgcagctatt aaacttgtcc actccattct
     3721 tccaaatgga ttttgctctt tatactcatt gtctacaaca taattaagcc ctaattcttt
     3781 taatttgtca ataaattttg tatcagcctc tgataaacca ctactcacta tagttaagtc
     3841 aggatttaag tctactattt tttcataatc tggtgctaaa tttgaaccaa cgtaatgtat
     3901 ttttccatct ttcattaaat ctttaatttc tttaatagtc cagtactgct cttgtgttgt
     3961 tacggctttt atactatcta gttcatctat tgttcttaga gaacaagcct gtgttgtaga
     4021 tgctattaat acattgtcca caggcgttct aactatatta gcatttttat atttttcagg
     4081 aattttaatc ttctttggga ttaaaatcag ttctcgatta tctccatcag ttactttttt
     4141 aattccatct tctaaatctt caacactaaa tccactagca tattttaatt ctaatttctt
     4201 accagtacta gcttgtttat tctctttgtc acttttacta caccctataa gacctcctac
     4261 cataaaaatt accatagcaa ttaaaaaaaa ctgctttaaa acatttgatt taaatttcat
     4321 ctctttcact ccaatatctt attttttatg agtggctaaa aaactttaat aatatatgct
     4381 ttttaaagta tacttattaa agttatccta aagtcatctt taataagtat aaccttttaa
     4441 taaaataaaa aacttctcct aatttaataa ggagaagttc atatatcaac tttacaaaaa
     4501 cgaattgata cttcatttta ttataaatta caatctccac cctaaattga acttatataa
     4561 tactgggcag gtctcctgac tcacggcttt tcctccatca cccttcccaa aatttttata
     4621 aatttcagtg gattttgatt tcgtatccgt atacagtagc gggggctgta gtgaattttc
     4681 atcactttcc ctattaaact aatatagtac cctagtatta ttttaattta atttttatta
     4741 agataattgt aatttattgt tgacctcttt gcaaggttga tttaacatat atttaattca
     4801 ttcatcaatt ttttaatttc acatacttgt ttttcatttg gttttacaaa tggagcagtc
     4861 atataatcac ttatttgtaa ccctctgata ttcatagctt ttttcattgc tggtataaat
     4921 ggattggtta ttaagtaaaa atccattgct ttattaatat atttttgaat ttctataacc
     4981 ttctccatat ctctattatt gtaagcttca gcccatttgg agcaaacttc tggaattaaa
     5041 tttgataacc cccctataca acctgctccc ccagacatta gattatgtat aaagttttca
     5101 tcataaccac tataaacctt aaattctggg aactctattt tcatagtatt tattaaatct
     5161 ctggtatgtc ccatcaaagt tactgtatcc ttatatccaa ctatgttttt atgttttctt
     5221 gcaagtctta atgtaagttc tggacttaaa tcataaccag ttctatctgg ataattgtac
     5281 aaatatattt cagcctctgt attcttagct atttcactat aaaataactc aatactatca
     5341 tcatcaagag agaaataata agggcttatt atcataacac catctgctcc actattatgt
     5401 gcatagtttg aaagttctat agattcattt atacacatac gacttgtacc tataaacact
     5461 tttactctct tatttacatg actagttact aaatctataa gttctttttg catatccata
     5521 gacatattaa agaactctcc agtacttccc attatgacta gtccatctac acctccatct
     5581 ataagatggt catacacttt tttattacct tctttatcta aatttccttc ttcatcaaat
     5641 attgtaacaa ctggtgtcaa aaattctgct ttcataattt aaactcctaa aatttatttt
     5701 aatatgttca ttaatacatc agatttagct ttaatattaa ttatattaaa acagttactt
     5761 aatgtaattt tttaaaattc caatactctc aatctcacat tcaactacat ctccactact
     5821 catatatcga ggaggtgaaa atcccatccc tactccagaa ggtgttccag tagctataat
     5881 gtctccagcc tctaaagtca taccttttga aagttctgaa actatgtctg aaacgtctga
     5941 tatgaataac cctgtattag aactttgtct ttcttcacca tttaccctac tacatacttt
     6001 aagttttatg ggaaaaggaa ctgcactttt atgtaaaata catggaccta aagaggtaaa
     6061 tgtatctaat ccttttcctc taaaccattg aacatgttct ttttgcaact ttctagcaga
     6121 aatatcattt ataacagtgt aaccaaagat ataatcttct acctcttctt ttctaatatt
     6181 aacacctgtt ttacctatta ctactccaag ttcaacttca tagtctagtt gattatttat
     6241 ctcaaaatga ccatctatct catcttctgg acctattgcc tttgtcacac gttttgtaaa
     6301 ataaaccgtt ttttgtggtt cttcaaaact atcatcaaag tgagtttgag tttcttcaag
     6361 atggtcttta tagttcactc ccacacataa gatatcatgt attggttttt ctataggaac
     6421 atgtacagta catctctctc ttttaaaaac atcataatta tagaatgtac tttcatttat
     6481 aatagttttc aacttaataa tatcttcact agatacattt tgaattaatt catttaaatc
     6541 cttaaaattt ctactgagat ttaaagagtt aacatctata ataaataatt catctttaga
     6601 aaaaactcca gttcttttac attcattttc tccttcagta aaacttacaa acttcataat
     6661 ctcacccttt attaaaattc tatgtattta ttttcttact taaagattat acatagttgc
     6721 atactctagc tattgccatt tcagtatatc caagggactc tgcttttgat tgttttccat
     6781 ttgctacatc tacaaccata tttaacaaat catctgttaa ttcttccatt gtttgtggac
     6841 catatataac tggactagca tcaaaatcca gattatcttc catatttgca aaagttattt
     6901 tatttccagt tatttttatt actggtgcta ttggatttcc tgatggtgtt cctcttcctg
     6961 tactaaatac cactatttgt gctcctcctg ctaccatacc agcaacagat gaagcatcat
     7021 ttccaggggt atccattata acaagacctt tcttatcaat ttgtttggca taatcatata
     7081 cttcacttat ttgtctatga ccacctttat gtatacaacc aagtgatttt tcttctaaac
     7141 ttgttaaacc accagctata tttccaggag atggattacc ttctctaact tcttctccaa
     7201 ccaattttaa tgaattttca tatctatgta ctatatgtaa tattttttct tttacttctt
     7261 catttactgc tctcctagct agtatatgct ctgcacctat aaattcagta gtttcactta
     7321 aaattgatgt tgctcctaaa tcgacaagtt tatcacttaa ttcacctatt aatggatttg
     7381 ctgctaatcc acttgttggg tcagacccac cacattcagt tcctagtata agttcagata
     7441 ttggaaattc ttcttttcta agcaaagaag cttcttgtgc catttctcta gcataacgaa
     7501 cagctttttc tatagttttt aaagttccat gttcttcttg tattacaaga gtttttattg
     7561 gcttatttgt tctctctttt attgcatcaa ctaccaaatc attttggcaa ttttcgcatc
     7621 ccaaagatac aactattaca ccatatacat ttggatttgc tgcataacca gccataacat
     7681 ccatagttaa ttgttggtct gaatgtactt gtgaacatcc attctgatta ttaaaggtaa
     7741 ctgccccttg aacttgtgat gctattattc tagttgtatc tgatgcacac acactcgtag
     7801 gcaatattaa tatgtggttt cttataccaa ctcttccatc tggtctttta tatccataaa
     7861 atttcatact gatttcctcc taaaatagtc tatatacttt ctataatttt tatatattat
     7921 aatttttatt acactcacta atcaaacagc taaaaaccta tttaaaaact atttgacact
     7981 aaaatcaatg aatacaactt ttataactct ataaatatta ttttatatat tagtcataac
     8041 ttgtttatcc aaatcctctc taacacttct aacattatga atatgtacat gttgaccact
     8101 ttttatctcc tgatttgctt ctcctatatg ttcaccatat ttagtaactt tatcaccttg
     8161 ggccatatct tttatagcca atttatgata aatagttata tcatctagtg ctgtaatcgt
     8221 ttttactgtt ttgtctttta atttaaaact aatttcacta cctttagcta ttgcttcaat
     8281 agctactgca acattatctt tttcatcaat tatcactgca tttatcatta ttcttgctct
     8341 cctttataaa taatataatt ttattaatat cctaaaatct aattaaagaa tttattattt
     8401 taccaacaac tcactgtaat ataaattaag ctgttattgt accatcctca ttttgttttg
     8461 ggaacttagg acatccatac ttcttagccc accaactagt tataagaggt actaatattg
     8521 cagtgacaac tactgcggct gccacttgtg ttgtagctgt tgcaacatat ggttgccatg
     8581 atgggtcaac taatgcaaca gcagcaggta cagcaattgc atttccagca gttgtagcta
     8641 cagcccatcc tgcatatcct ggacgttttc ctataaattt atcacagaat actataaata
     8701 tacctcctac aaatacagat attaatccaa gtaatactcc tgataaacct ccttttagta
     8761 tacttgcaag atttatgcta ctaccaagtg caaaccctac aaaaggtacg agtaaattac
     8821 accctggctc taaaaactct ttcatttttt tgtcaaaatt tcctattatc ataccaacaa
     8881 gtattggcac tattgcagcc aataatgaca acaatggtac atttgcaaga cctgaagtcc
     8941 caagagctat taatgtaaaa aatggtccat catttaaagt aagtaaactc atagttgcac
     9001 aatctgtttc atctccatat gaagtcatca atgaaagata tatactacca ttactatttg
     9061 taactgcact tataacagcc aatgttgtaa gaccaaggac accatccatt ccaaatagct
     9121 ttcctataag tatacctatc aaagcaccta tagcaaattt agaaccaaga agtataccac
     9181 ctctctttaa aacttttggc atttctctaa attgaagctg agttcccaag cataccagtt
     9241 gaaccccaat tatagttgca gacccaacac ttgaaaatac tgctgtcgta aacgaaccta
     9301 tttgtacaat atctgggcaa aaggtattaa tgaaagctgc tataaacatt ggcactatca
     9361 tcatacctgc aggaattttt ttaataaaat ccattatcat aaagttaatc ctcctaaaac
     9421 atgatattta attactctta ttaagttttt agctttaatt ctaacggccg tttcggttcg
     9481 tatttaagaa ctaagttttt aattacgaat ctttgaatta atgatagcat tttcctattt
     9541 atgagtcaat aaaaaaaata aatttttgtc aaaatttttt caaactttct gataatacct
     9601 tgtatattat caaatgactt ccttatatgc aaaaatacag cgtatttaaa attatatagt
     9661 attcaaaact ataaagtttt tatacagtgt aaaaaataga taaaatataa gttcataata
     9721 ttttgatatt tacatataaa aataactatt gcatatattt acttaaatta ta
//
LOCUS       DAYASV010000138         9761 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.142, whole genome shotgun sequence.
ACCESSION   DAYASV010000138 DAYASV010000000
VERSION     DAYASV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.142"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(154..1110)
                     /locus_tag="ACGI68_003154"
     CDS             complement(154..1110)
                     /locus_tag="ACGI68_003154"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0139575.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            1813..2808
                     /locus_tag="ACGI68_003155"
     CDS             1813..2808
                     /locus_tag="ACGI68_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0139576.1"
                     /translation="MSIIELNELTKAYRRFEKKAGFMGSVKSLFNRKYTEKTAILSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNHFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            2795..3649
                     /locus_tag="ACGI68_003156"
     CDS             2795..3649
                     /locus_tag="ACGI68_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139577.1"
                     /translation="MSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFDVVV
                     NMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSEEIC
                     NRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFTSNP
                     VQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFFKMIIENIVEFITGTMIPLVLL
                     PETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYKRMR
                     TLFDGVGI"
     gene            3649..4440
                     /locus_tag="ACGI68_003157"
     CDS             3649..4440
                     /locus_tag="ACGI68_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HFH0139578.1"
                     /translation="MKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLMF
                     NSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYITK
                     PLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLLF
                     YDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMST
                     IPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            4725..6104
                     /locus_tag="ACGI68_003158"
     CDS             4725..6104
                     /locus_tag="ACGI68_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0139579.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTVSGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTIFCIC
                     KYKNRYKYM"
     gene            6552..7265
                     /locus_tag="ACGI68_003159"
     CDS             6552..7265
                     /locus_tag="ACGI68_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139580.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(7537..9075)
                     /locus_tag="ACGI68_003160"
     CDS             complement(7537..9075)
                     /locus_tag="ACGI68_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0139581.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         3425 a         1141 c         1456 g         3739 t
ORIGIN      
        1 tattctataa tagtggtcat gttgactatt gtagttttat taagaatagt tggtttatat
       61 aaatatatgc aaagatagct tgttgtttac tttacttatt agtatatagg acaagatact
      121 ctaataaatt tgcctatttt aatatctttt gtactaaaac caactaaagt ttcctttatt
      181 atttcgtttt gaataataca aagcataatc cgattgtttg tataaagact ggaaagtatc
      241 agagttattt tctaatttag atatacctat agaacaagaa ttactctcta ggcataattc
      301 taaaaccttc tttgccttat tttcaacatt ctcttttgat tgattactat attcaaaaac
      361 agcaaattca tctccaccaa gtcttccaat aatagcatca tttacgaata ctttagtcaa
      421 aatatttgaa aaatgtatta gtatttcatc tcctcgaaca tgaccaaaga tatcatttac
      481 ctgtttaaaa ttatctaaat caaagatgta aaagtatcca tgtgaaacag ttttaagcat
      541 ttcttttatg tgtacttcaa aaaaatgtct attgtatagt ccagtcataa ggtctttttc
      601 tgattttagt ttaaattcat ttgccattgc tagtgcttct tgtgcttttt cagttattgt
      661 tttagggtaa tactcatctt tcatttggtc aaagtttgga atagaatggt gaacatggat
      721 aagtttaatc atatcatctg taactctgta taccatagta aatcgagtat ccatttctac
      781 atagatatct ttttctaggt aatctttttc tattacccaa agagtaccat atactataaa
      841 agcttcgtct gtgactttgg tacatgaata ccactcatct ataatctcaa agtctatttg
      901 ctctgattct gtttggtctt tttcaagacc tataataaat tcttctaatg tttcgtaaaa
      961 ttcatgtttt cctgttccaa ttaatgcaag gttgtcatct aataaggaaa taataaattt
     1021 aaggttttct tcttctggat ttaaaatata aatatgccaa attttttttg ataaatctct
     1081 taattgtttt tgtattgtat tattttccat agattacccc cttaagattt acagtttata
     1141 ttttaatgta ttataagttg aactcttatt acaatattat tataatatag aactaaagaa
     1201 attacatcct gtataggtaa aagtatatct tttacaataa aaatattaca gtaaaatgac
     1261 cagttttaat atacgtatta tatttttaat ttataaacga atattataaa tgaaatttaa
     1321 attcaacctt gaagaaataa gctaaatgta ttagtatatt aatccatgct tgaacataaa
     1381 taacctttat gatatgatat caataataat attatgagag gagtgccttg atgtcggagt
     1441 tatataatgg taaccaacaa ttaaatagtt ggagacacat atattctgta taatcagaaa
     1501 tttcaagtgt atttttaata catttaattt ttgtgtctta ttctgccttt atagataact
     1561 gacatatgac aacaaatagt atttaaaata tatattactt tgttaaatta tggtgtagta
     1621 ggctttaatc tgctacactt ttattaattt tgtatagtat tattttgata actattttga
     1681 aatagagagc attaattttt ctagtccaca ggagttttct gtttttgtgc tgttaaatat
     1741 aagcacagaa aaaggcagat atatacctgt ggactttttt tatataaatt atgaaatatg
     1801 gaggtagtta aaatgagtat aattgaactt aatgaactga ctaaagctta tagaagattt
     1861 gagaaaaagg caggatttat ggggagtgta aagtctttat ttaataggaa atatacagaa
     1921 aaaacagcta tattgtcatt tgatttaaag atagaagaag gagaatttgt tggattaata
     1981 ggtcaaaatg gagctggtaa aactactctt attaagatgt taactggtat tattgcaccg
     2041 tcttctggag aaatatctgt aatgggttat taccctaaca aattagaaaa taattttaaa
     2101 aaacaatatg ctgttgttat gggccaaaaa agtcaactat tctttgaatt gacaccagca
     2161 gatacgtttc ttttatttaa agaattatat gatatatcta atgaagaata taaaaaaaac
     2221 ttggattact ttatcgaatt atttgaggta tctgactatt taaatgttca agttaggacg
     2281 ctttctttag gagaaagaat gaaaatggaa ctaatagtag cccttctaca taatccaaaa
     2341 atattatttt tggatgaacc aactataggc ttagatgcta tagcccaaaa acaaattagg
     2401 tatttcctaa aagaagtaaa tgaaacaaaa ggaactacta ttatacttac ttctcattac
     2461 atggaagata ttaagagtct ctgtagtaga agtattgttg tagaaaatag ccgtaaaata
     2521 tatgatggtt ctacagagaa actatttaat cattttcaaa aatataaaaa aataacagtt
     2581 agtttttcca atgaaactaa cattgtattt ccaaaagatt gtaaagttgt tgaacaaaca
     2641 aatcataaga tatcagtaat tgtgccaaaa gaaagaggaa atgaaacgat taaatggatt
     2701 tttgataaat atgatgttaa agatgtaatg attgaagaag aagatatagg tagtattgta
     2761 gagagaatat ataagaaagg aagtgatgag tattatgagc aactataatg aagcaagttc
     2821 aaatgattca atgttttata aatttaaaca gagtattaga aggtattatg aagtagccaa
     2881 aattaacttt aaagtgcaac ttatctggag atttgatgtg gtggttaata tggtgtttac
     2941 gattgtaaaa attttatttg cttatatttt atggaaagca atttttggtg aaaataaaat
     3001 agtctctggt tttacattta actctatgtt gtcttactat ataataagct catttttagc
     3061 aggtattgat atgtcaaaga gtaccagtga agagatatgt aatcgtgtaa aattgggcac
     3121 cttttcaaag tacatgatta ttccaattaa tgttcaaaat tattttattg ctaaaaattt
     3181 tggaatgaca tgtttttatt ttgtatttaa tatgatttct gcatttgtat ggatttttat
     3241 atttaaaata gattttgcct ttacatcaaa tccagttcaa atattaattt caattttgat
     3301 gatttttcta gggtttattt ttatgatgaa actccacttc tttattggga ttttgtcatt
     3361 taaatttctt gagattagtt tttttaaaat gataattgaa aatattgttg aatttattac
     3421 aggaacaatg ataccacttg tactcttacc agaaactata gtaacagtta tgaaggtatt
     3481 tccattttat tatacaacat atttgccatc tatgttacta ataggtagaa acagcaatga
     3541 ggcaatacaa ggattaatta ttctagcatt atgggtttta tttttttctg tagtaaataa
     3601 agttatgtat aaaagaatga gaacattatt tgatggggtg ggtatatgat gaaaaaaaat
     3661 ttgaaaataa tatttatgtt attaaaaatg aaattatcaa agatgatggt atttagattc
     3721 gatttttttg gagcattttt tgtagatagt tcattattta tattacaact tctaatgttt
     3781 aatagtatat attctcatgt agactcaata gggggatggc aacaaggaga gatgttaata
     3841 tttataggaa ctttctcatt aataaatgcg ataaatatga cgattttctt ttttggtatt
     3901 tatgatattc cacgaaaaat ccaagaagga gaattagact actatattac aaaaccatta
     3961 gacccactat ttaggttaac atttgaaaat attaatattg gttcatcacc attaattcta
     4021 gcaagtatac ttattgtttt atatggaata tctgagttaa actcagagat aactttagtt
     4081 atgataataa tatatatttt catggtgtta ttgatgacat tactttttta tgatgtatgt
     4141 atcatactta gaacagtttc attttttgtt atatcatctt ctgcgataat gagattagaa
     4201 gataatttat tgccaatgaa tatgaaaatt cctggtgtaa tatatgaagg tgtttttaaa
     4261 gtattattct atttaatctt accatatgga attatgtcga caattccgac tcaaattttg
     4321 gctgggacaa taacaattaa tggattaata tactcaattt taattgtatt tttgtttaca
     4381 ttgtttatgt ggagattttg gaagttaggt atgagacatt ataaaagtgc aagtagttaa
     4441 taaattagta aaaatagaca atgtataact atatataaat acgtttcata tgacactaaa
     4501 ctatgtgaac aagtatttgc gctaaaggca ctgaaagcta taaaattatt aaagtaagga
     4561 tttaaaagtt tctttagtag aaatataatt gtgtaaaaaa gcaagattaa ttaaaactat
     4621 atccaaataa ttagatataa ttcttatgca gaaaggaggt ctgtaaatgt ctaattgttt
     4681 ggaatatatt ttaaaaataa ttaatgtaca gaaaaataaa caaaatgagt gatgtatatt
     4741 attcagaaaa tcatgaacaa gataatcttt atgagcaaga taatttactg gcacaaagat
     4801 ttaatttatt tactctattg aagtttgcat ttcctactat ttttatgatg atttttatgg
     4861 ggttgtatac aatcgtagat actatatttg tatcaagatt tattaataca aatgctctat
     4921 cagcaattaa tattgtatgt ccaattatta atcttatagt tggaattgga actatgcttg
     4981 ccactggagg aagtgctatt gtagcacgta agatgggaaa tggtgatatt gagggagcaa
     5041 ataaagcttt ttctttaatc gtattatcag gagttgtaat tggggtaata attactggta
     5101 taggtattgt atttatggat tatatcatta tatggttagg agcaagtgaa gttttaatta
     5161 aatattgtcg ttcatatctt tttattatct tggtttttgc ttctgcaaat atactacaag
     5221 tattatttca atgtttattt gtaacagctg gaaagcctca atttggatta tggctagtgt
     5281 taggtgctgg gtgtgctaat ataattcttg actatgtgtt cattgttctg ttacaaatgg
     5341 gtattagtgg agcagcactt gcaactggag taggatacct aattcctacc gtttcaggta
     5401 tggttttctt ttcaaaaaat aaagggttac ttaaattttg tatacctaga ctagataaaa
     5461 gaatattatt tgaagcttgt tttaatggtt catctgaaat ggtgggtcaa ctatcaacag
     5521 cagttactac ttttttattt aatgctatga tgatgaaatt aattggtgaa gatggggttg
     5581 cagctattac aataattatt tattctcaat ttcttttaac ttcgctttat ataggtttct
     5641 caatgggtgt tgcaccaata ataagttata actatggaag tgaaaataat acgcagttaa
     5701 atcatgtttt taagatttgt atattattta tttcagtagc atctttattg gtatttatct
     5761 tttctatatc atgttcacca atcattgtag agattttttc tcaaagagga agccgagttt
     5821 atgaaattgc aaggtctgga ttttatatat tttcttttag ttttattttt tgtgggtata
     5881 atatttttac ttcgtctatg tttacagcat tatcaaatgg aaaggtgtct gcaatattat
     5941 cttttctacg tacatttgga tttataacta taggtttatt gatattgcca aatatattaa
     6001 atataaatgg gatatggtta gcagtaccaa gtgctgagat tttaacttta tttttaacca
     6061 ttttttgtat atgtaagtat aagaatagat ataaatatat gtagatatta aataaatagt
     6121 ttaggtagat attttatgtt ttaataagta attcaattag tgggagatat atactattga
     6181 aaattaaagt tataattgat ataaagaatt aataaaaata acatataaaa gtttaaaagg
     6241 gagttgaact taaagtttga ttagaaaaag tcaattttaa gacaactcct tttattttac
     6301 tattgtatta atctgtatct gtaagtgtat tagtctaata ttataaactt tggatgaaat
     6361 taattttcta taggttttaa tgtatcatac actttatttt aaagaaactt atcttaattt
     6421 tcatagtgat tgaatttgtc tattagagat gttactatta gtaatgtaaa agcaagagta
     6481 gtgaatataa aaaacaagat agtgatactg caagattaga atataataaa tttttatgga
     6541 gagatttcaa tatggataca tgttttaata aaaaaatatt acttgttgat gatgaaaagg
     6601 atatagtaga tttaattgaa gaagtactca taaatgatgg ttttaaaaac attattaaag
     6661 cttataatgg tctagatgct atttcgctgt gtaagatagc atgtccagat gttgtcattc
     6721 ttgatataat gttaccagat atagatggta tagaagtgtg taaaaaaatt cgagagtttt
     6781 catactgttc tattcttttt ttatcatcaa aaaatgatga tattgacaaa atactaggtc
     6841 ttagtagtgg gggagatgac tatataacaa agccattcag tccaagagaa attgcttttc
     6901 gagttaaagc gcaattacgt agacaacagt atcaaagcat tgtaccatca gatagtgaag
     6961 ttattaaaat aggagatatt actattgata tagaaggaaa ccgtgtatat aaagatagaa
     7021 atgagattga gttaactgga agggagtatc atcttttaag ctacatggct caaaatgtaa
     7081 ataaaattat aggaaaagaa agactctatg aacaagtatg gggagtatat agtagtattt
     7141 gtgacaatac aattatggtg catatacgac atataagaga aaaaattgaa gataacccct
     7201 caaatcctaa aatacttata acagttaaag gactaggata taaacttgtg aatagaatcg
     7261 attagaatag attttaatat taatgaagat atctcaagtt gaatttttag ttaatgaggc
     7321 attcttttat gggtaaaatc aaatatattt tcgttatctc aacaatgaag aagaggtcta
     7381 tctctatttt gagatagcct cattaagttt atatcctggt aatgtacttt atcttctaat
     7441 aaaatttttt tagtttattt gcagtgaaac attaactaga acattaaaat ttgcattgaa
     7501 atactaatta aataatattg tatattgtgt ataaacttat actaattgtt tttctatatt
     7561 aaatacttta tcaatccaac ctttataaaa tttttcttca tgggaaacta ggattatact
     7621 tcctctaaac tgtttcaaag cattttgtaa agcatccttt gtctctgcat caaagtgatt
     7681 ggttggttca tccaggataa gaaaattaca aggagataac atcatacaac ataatttaac
     7741 cttagattgt tcaccaccac ttaaagtaga tacactcctt gaaacatgct cttccttcac
     7801 accacatctg gctagatgac gtctaatctc ttttgtattt aattttggat atttatctgc
     7861 tactatttga agtggggttt tatcaggatt ttcccatttt aaatcttgct cataataacc
     7921 aattttaact tgttctgaaa actggaaatc tccagatata cgaggaatat ctttgactaa
     7981 tgtctttaac aaggtagatt taccgattcc attaaaacca gttataacta ttttttgacc
     8041 tccatcaaca gagaaattta attttggaag taaagaatag tagtatccaa cttcaagatt
     8101 tgttatagta agagcatttt gagccgatat ttcaatttct gaaaattgaa tatttggttt
     8161 tcctgtaaaa ctaggaggag ctattctttc tattttatcc aattgttttt gtcttcctct
     8221 tgcaattttt gaatttactc cagctttatt tttacgtata aatgtttctt ctttttctat
     8281 tttcttttgt tgtgcttgat accttctaat ataatcttct cttaaatgtg atttttgttt
     8341 aaggaactct gaatattttc catgatattt cttaatcatg ccaaattcta tatcaagaat
     8401 acctgtagaa attttttcta aaaagtcaaa atcgtgggaa actacaataa atgccccatt
     8461 aaatgtattt aaataatttg agagccattc aacatgttct ttatctaaaa agtttgttgg
     8521 ttcatctaat aatagaacat catggttaga aagtagtaat tttgctaaaa ttacctttgc
     8581 cctttgacca ccactaagct ctccaacaac acgattcatt cctattgaat ctaatccaag
     8641 accatttgct attttattta tttcattatc tatagaatag aaattacttg ctattagttg
     8701 ttcctgataa tctgatgctt tgattagata ttgctcattt tcagatatag cacttttttg
     8761 atagagtaaa ttcatttcct tttctatttt ataaagttct tcaaatgcag tgtgtaaata
     8821 gagtgatatt gttgtatctc tattgatttc tgcatattgg tctaaatggc ctatattgat
     8881 attaggttgc cacttgattt ctcctgaatc tggaacaact tctcccaata aaattttaat
     8941 taatgtactt ttacctgtac cattttggcc aactacacct atatgttcac ctttgaataa
     9001 atcaaagtta gctttctcat acagtgattt atccataaaa cattgtgata aattagttac
     9061 ttttaataaa ctcataattc aaactccttt tcttttatta aaaaatttat ggaaataaaa
     9121 aaagcagagg aatggcattg taaaatgccg acctctgctt ttggaaaaac aaaaggtcat
     9181 agacaagcat tgttgtctat gaccagaaaa tctatattat ctatcggcaa ataggtaatt
     9241 ttaaataagc atagccaaaa aactatacta aacctatatt tgccgatgaa gatagaaagc
     9301 tttaagcttt cagattaacc tctacacaac gcttcatcgt atacatttgt gtagagttaa
     9361 gttttctaac aagtttaact aattctatat aaatcataat tattcacatc ctaatcttag
     9421 tattaattct agtttatctg tatttaaatt gtacttttgt actttgtgga ttatcttatt
     9481 tatttaaaat atgttttatt aattatagct taagttatgt tatcataata cttagttata
     9541 gtcaataaga aagttatatt tactattatt atattaattt agtaaatcta tcattacaaa
     9601 gttatttgaa gactcgctat caataaaatt ttatagtcta taagaaaatt aagattatga
     9661 gttttataat tttgatatag ggatactatt ttcataaata ttacaacatg tagaagtata
     9721 tgtctattat tgttgaattt tcaatgatat attaaggttc t
//
LOCUS       DAYASV010000139         9720 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYASV010000139 DAYASV010000000
VERSION     DAYASV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.144"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            111..1031
                     /locus_tag="ACGI68_003161"
     CDS             111..1031
                     /locus_tag="ACGI68_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFH0139582.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            1146..2303
                     /locus_tag="ACGI68_003162"
     CDS             1146..2303
                     /locus_tag="ACGI68_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFH0139583.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(2388..3887)
                     /locus_tag="ACGI68_003163"
     CDS             complement(2388..3887)
                     /locus_tag="ACGI68_003163"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFH0139584.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            4483..5907
                     /locus_tag="ACGI68_003164"
     CDS             4483..5907
                     /locus_tag="ACGI68_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFH0139585.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDSTSLEEAKAKMSDMKSASKNSEKNIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            5933..8071
                     /locus_tag="ACGI68_003165"
     CDS             5933..8071
                     /locus_tag="ACGI68_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0139586.1"
                     /translation="MDNLKKKKITSRQKKIILMIVANSKKNIPITISEIAGTLELSSR
                     TVLRDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEER
                     NLIILSKLLVSNEPVKSYYFAKILKVSEWVLNNDFALASKWLERFDIELVRKPGLGVY
                     LKGQEKNFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCI
                     IRKVEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITE
                     EFKLAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITN
                     KMIDLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYD
                     VVEKISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSR
                     FLSTKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSL
                     DDEQIIKEKIKSIAQNKLINLNAKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEK
                     FESKDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCMSERID
                     IMKLSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKI
                     NKFSIEEMALEVKDIVFNFYTNRLKAFIDK"
     gene            8101..8523
                     /locus_tag="ACGI68_003166"
     CDS             8101..8523
                     /locus_tag="ACGI68_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0139587.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            8542..9693
                     /locus_tag="ACGI68_003167"
     CDS             8542..9693
                     /locus_tag="ACGI68_003167"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0139588.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEVHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSIGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
BASE COUNT         3945 a         1143 c         1643 g         2989 t
ORIGIN      
        1 tttgaaacaa tttgtagaat ttcaaattcc ttgacagtaa taagtaagta attgtataat
       61 caaactgcaa tatattaata atattagtga aaatgaaagg attataaaat atgtataaat
      121 cagaaaattt ttcaaaaaaa tctgtattaa taaattcatt tttaagtaaa tttttaaaaa
      181 taattggatg gggtttggta agcttattta cattatcagc cttagtactt tcaactattt
      241 ccattataat tggatgggct gtaatacttc ctattcaagt atgtgcagta tttttactta
      301 taggaggagt ttgtattttt aaaggtaaga gattaggaga ccaaatttct cgttataaaa
      361 aatattgtac aattataaac aatagaaata ttattccagt agaacttatt gcagaaaaca
      421 catctaaatc attaaatttt ataataaaag atgtacaaaa gatgatagac aaaaactact
      481 ttataaatac ttatattgat aaaagaaata atcagattgt gttaacaaat gaagattttg
      541 ttccaccaag atatgaagat gtagcatatg aagtcaaaga agaagaaaag actgatgaat
      601 ccaatgaagt tgatacaatt ataaataaag gaatgaatta tctaacacaa ataaaagaag
      661 ctaataaaaa tattaaatca gaatcaatgg gcgataaaat tgtacaagta gaagatgtta
      721 caagtaaaat atttgatgtt gtaaaacatg acccatcaaa acttactcag atacaaaaat
      781 ttatggatta ttatcttcca accactttaa aactattaaa ttcatatcat acattggaag
      841 aacaaggaat tgatagagaa aatataacaa caactatgga aagtattgaa aatacaatga
      901 atacgattgt agtagctttt gaaaatcaat tggactatct gtttgaagat gaagccattg
      961 atatttctac agatattact gttttagaga atatgttagt ccaagaagga ttaacgagtg
     1021 gtaaatttta acttcaatag aaaaaatatt ctgttatttt taaacaatta attagtcata
     1081 aagtataaaa tgtgtattat ataagttaat cagtacttta tgaaggaaga ggagagaaag
     1141 atacaatgaa taatttagat gatataccag ttatgccaac tttgacactt gacccatttg
     1201 gagaagaatc aaacactaat gatatagata attctgactt attaatgaaa aaggatgaaa
     1261 aagacccaga agaagaaaaa ctgtctgaat ctgaacgcaa gatggtaaag gaatttgcgg
     1321 aaaaaataga tattacaaat acaaatatga ttttgcaata tggagctgga gctcaaaaaa
     1381 aggtagcaag tttttctgaa acagctttaa agagtgtaaa aacaagagat ttaggagaag
     1441 ttggagatat gcttacaaat ctagttactg atttaaaagc attttcagca gatgaaaaag
     1501 aacaaagtgg ttttttagga atttttaaaa aggcaaataa taaaatttca aatttaaaaa
     1561 ctaagtatga tagtgcagag gtaaatgtgg ataaagtaag caaagagctt caaaagcatc
     1621 aggttaaaat attgaaagat atagcaatgc ttgataagat gtatgaattg aacttagctt
     1681 actcaaaaga gcttactatg tatattatag caggaaaaca aaaattaaag gatatgaaag
     1741 aatatgaaat gccaaagttg agagaaaaag ctcgtttatc tggttcaaca gaagatgctc
     1801 aatctgtaaa tgatatggtt tcactttgtg acagatttga aaagaagatt catgacttag
     1861 aattaactag aatggtatcg cttcaaatgg ctcctcagat aagattggtt caaaatagta
     1921 ataatctgat ggcagaaaag attcaatcaa ctatagtaaa taccattcca ttatggaaaa
     1981 atcaaatagt gcttgctctt ggaatttctc acttaaatca agctatgaaa gcacagcgtg
     2041 aagttagtaa tatgacaaat gagcttttat taaagaatgc agaaactttg aagatgggaa
     2101 caatagaaac agcaaaagaa tcagaaagag gaattgtaga tatagacaca attaagaaaa
     2161 ctaatcaatc tctaatatct actattgatg aggtagttaa aatacaacat gatggtagaa
     2221 taaagagaca agaagcagaa gttgaattat caaaaataga aaatgaactt aaaagtaagc
     2281 ttcttgaatt tacagttaaa taaaaattat actaatataa aaagagactg tctcaaaata
     2341 gagataagtc tctttttcat tgtttaaatg attaaagtat atttatatta taaatttcta
     2401 ttatctttta acataacatc atgagctcca ccttcaacta tactagtaga agatacaagt
     2461 gtaagtttag catttttttg aagttcagga atgctaagtg ctccacagtt acacatagta
     2521 gaacgaactt tacttaaaga aagagttaca ttgtctttca aagaaccagc ataaggcaca
     2581 taagagtcaa caccttcttc aaaagaaagt ttcttatctc caccaaggtc atatctttgc
     2641 cagtttctag cacgagcaga accttctccc caatactctt tcatataact accatttata
     2701 ttaactttgt tagttggact ctcatcaaaa cgagagaagt atctaccaag cattataaaa
     2761 tctgcaccca tagctaaagc taaagttata tgatggtcat aaacgatacc accatcagag
     2821 caaattggaa tataaatacc tgttttttca aagtattcgt ctctagcttg agcaacttca
     2881 attatagaag tagcttgtcc acgtcctata cctttttgtt cacgagttat acatatagaa
     2941 ccaccaccaa taccaacttt aacgaagtca gcaccagctt cagctaggta aaggaatcct
     3001 tcgcggtcaa ctacatttcc agctcctacc ttaactgtat caccatattt ttcacgaatg
     3061 aaatcaagtg ttattttttg ccactcagaa aaaccttctg aagaatctat acataaaaca
     3121 tcagcaccag cttcaactaa agcaggaaca cgttcagcaa agtctctagt attaatacca
     3181 gctccaacta catatctctt agaagaatct aaaagttcaa gtggattttc tttatgagaa
     3241 gagtaatctt tacggaatac catatacata agattaccat tatcatcaag tacaggaaga
     3301 gaattaagtt tgtggtccca tataatattg tttgcttcct ttaaagtaac atctttgtta
     3361 gcataaacta tagatgacat aggagtcata aattcactaa ctttagtatc taaatccata
     3421 cgacttatac gataatcacg acttgcaaca atacccaata atttaccagt tgaagttcca
     3481 tcttcagtaa cagctactgt agaatgtcca gttttttctt ttaagtcaag tatgtctttt
     3541 aatgtgttat caggtttaat atttgaatca cttacaacaa aaccagcttt gtgggattta
     3601 acacgtgcta ccatagcagc ttcattttca atagtttgag aaccgtatat aaatgaaata
     3661 ccaccttctt tagcaagtgc aatagccatt ttgtcatcag aaacagattg cataatagca
     3721 gaagtcaaag gtatattcat ataaatatct gcttcctcgc cctttttaaa tttagtaaca
     3781 ggtgttttta aacttacatt agcaggtata caatcagtag aagagaaacc tggtacaaga
     3841 aggtattcac tgaaagtatg tgatggtgta tcaaaataaa acgccatttt ttaatcactc
     3901 cttatatttt tatacttttt ataattgata gtattaatta taacttattc tttaatttta
     3961 ttaaagtatt ttgtcaaata ttgagatttt tgaaaaattt aaactatttt aattaaattt
     4021 gtttaatata tattgcctta tagggtaaag ttgtatatta aataatgttt agtgtttaat
     4081 agtagctaat ttaaaattaa tctttagtga ttcatataat tacatttgta tagtttacat
     4141 tgataatatt actagagagt gcataaacct caaaaataca gataaattaa atctatatta
     4201 gtatatccaa aagtacattt aataaattta tttaactgaa ttattatttt catttctatt
     4261 aaatttaaat attttataaa aatagtatta tttacaaaat catatactcc aaaatactac
     4321 ataaataaaa ttaaacaaaa actaaaatga caaatgtcat tatctaaagt gacatagaaa
     4381 cggtttctta aagttgttac tataaaatat aaactctata ataaaagtat aacaaaaggg
     4441 aatcatatcg atatagaata taattcaaga ggaggtaaaa ttatgagtag ttctaatcaa
     4501 tcgacaataa agacatctat acagcgagta gggaaatttt taagtggtat ggtaatgcca
     4561 aatataggag cttttatagc atggggtctt ataacagcat tatttatacc aacaggatgg
     4621 atgcctaatg aaaaattgag tactataggt gacccaatga taaaatacct tttaccacta
     4681 ttgatagctt atactggtgg taaagcaata gcaggacaaa gaggtggcgt aataggtgca
     4741 gcagcagcta tgggagtaat tgttggtgca gatataccaa tgtttattgg agcaatgata
     4801 atgggaccat ttgcaggatg ggtaattaag aagtttgata aatttgtaga tggaaagata
     4861 ccaacaggat ttgaaatgtt agtaaataac ttttcaatag gtataatagg tatgcttctt
     4921 gcaatactag gattttatgc aattggacca gcaatagtag cagggacagc tcttatagaa
     4981 tctggagttc aatttatagt tagcaaaagt ttattgcctc tagtttcagt atttatagaa
     5041 cctggaaaag tattattctt aaacaatgca ataaatcatg gtatattagg gcctataggt
     5101 atagctgagg caaaagaagc aggtaaatct ataatgttct tactagaaag taaccctgga
     5161 ccaggtttag gagttctatt agcatattgg atgtttagta gaggaagtgt aaaacaatca
     5221 gcaccaggag ctgtaataat acatttcttt ggtggtatcc atgaaattta cttcccatat
     5281 attttaatga atccagtttt aatattggct acaatggcag gtggagcagc aggaatattg
     5341 actttttcaa tattaggagc tgggcttgta gcagcaccat caccaggaag tatatttgca
     5401 cttatggcac tagctccaaa gggaggatta cttccagtat tagcaggagt tgcagtggca
     5461 acagttgtat cattcttagt ggcagcacca tttgttaaga gagcttcagc aaatcaaagt
     5521 gaagaagaca gtacttcttt agaagaagct aaagcaaaaa tgagtgatat gaaatctgca
     5581 agtaagaact ctgagaaaaa tattgaggaa aaacagcttg aagtaaatga gattaagaag
     5641 attgtatttg catgtgatgc aggaatgggt tcgagtgcaa tgggagcttc cagatttaaa
     5701 aatagaataa aaaacttaga cttaaatata gaaattacaa attcatctgt tgataatctt
     5761 ccagatgata cacaaatagt agtaactcac aatacattag ttgaaagagt tgctaagaat
     5821 aattcaagtg ttgagatagt ttcaataaac aattttttaa atgacccaaa tttagataca
     5881 ttgtttaaaa gattagaatc taaataaaaa taaaaagtac cactaattag gagtggataa
     5941 tttgaaaaaa aagaagatta catctaggca aaaaaagata atactaatga tagtagcaaa
     6001 ttcaaagaaa aacattccaa taactatttc tgagattgca gggactttag agttaagttc
     6061 aagaactgta cttagagata tgtctggaat tgaaaaatgg tttgatgaaa atgattttaa
     6121 ctttgtaaaa aaaccaggag tgggattgat tttagaggaa aatatagaaa atcaaaactt
     6181 tataattgag ttgttagaag aagaaaaaat agaaaaagaa tacagcaagg aagaaagaaa
     6241 tttaattata ttatcaaagc tactagtttc taacgagcca gtaaaatcct attattttgc
     6301 aaaaatatta aaggtatctg aatgggtact gaataatgat tttgccttag caagtaaatg
     6361 gttggaaaga tttgatattg agcttgttag aaaaccaggg ctcggtgtct acttaaaagg
     6421 tcaagaaaaa aactttagag aggcatatgt aaatcttata tacgattcat tcaatgaaaa
     6481 agaaatacta gatatggtca gaaatataag tgaaaatata caaacagata aagcaataga
     6541 aattttaagt gaaaatagac ttttaaattt aatggacagg tgcataataa gaaaagttga
     6601 agctacatta acaaagaagc tttcagattt agatgtaaat ttagctgata gtgcatatat
     6661 agggcttgta gttcatattt cattagcctt acagaggata aaaaatggtg aaaatataac
     6721 tatggataaa gagtttctaa aagaattatc tataacagaa gagtttaaat tagctaaaga
     6781 aatcgtaaaa ggtatggaaa cagattttaa catggatata ccagtagatg aggttggata
     6841 tataactatg catataagag gtgcaaagca aaggtcatct tcaaaccata aggctttaaa
     6901 cttagatgat atagaaataa tggaaattac aaataagatg atagatttgg cggaagatga
     6961 atttaaaata agtttaaaaa atgacgagag gttatttaaa gacctagcaa atcacttagg
     7021 gccttctata aataggttga atatgggatt agaaataaga aatcctcttt tagatgaaat
     7081 aaaaagcaag tattcatatg cctatgatgt agttgaaaag atttctagaa ttataaaaga
     7141 taaactaaat ataaattcaa tacctgaatc agaaatagga tatattgcaa tgcactttgc
     7201 atcagccata gaaaaaaatc ttatgatgaa tactaatata aatattgttg tggcatgtcc
     7261 cactggaata ggtacttcaa gattcttatc aactaagatt gaaaataaat ttcctaattt
     7321 aaatatactt gaaaccatat cagctataaa catagatgaa gagtatttaa aggaaaaaga
     7381 tgttgattta atagtgtcta cagttgagtt aaatacaagt ttaaattata tatgtgtagg
     7441 tccatttatg agtttagatg atgaacagat aatcaaggaa aaaataaaat ctatagctca
     7501 aaataaatta atcaatttaa atgcgaaaaa tgatactaag agtaagaata aagtatatga
     7561 acagataaca gaaagtatga atataggaaa agatattctt caatttttag aggaaattag
     7621 atttgaaaaa tttgaaagta aagatttaag tgagcttatt gaagactcat caaaaatatt
     7681 tgcaaaatct agtgaagata taatctctat aaaagaatcg ttaaaagaaa gattaaaaat
     7741 ttcaattcca tatatagaag aatcaaaaat tctattgtta cattgtatga gtgaaagaat
     7801 agatattatg aagttatcaa taataaaact agagaataga atcgtgctag attcaaatga
     7861 agaaatcgac aatgtagttt ttatgttact tcctaaaaat tcaccaagtt atcaaagaca
     7921 aataatgagt gaaataagtg gttcattaat agacaatata atttttacta ataagataaa
     7981 taaattctct atagaagaga tggcattaga ggtaaaagat atagttttta atttttatac
     8041 aaacagatta aaagcattta tagataaata aaattaatat taaaaaggaa ggttgatact
     8101 atgagtaaag tattgaatga gaataatata tttttagggt tagatagtgt ttcaaaggaa
     8161 gaagctataa ctttagcagg aagaaaatta gttgaaaatg gatatgtaaa agaagaatat
     8221 ataccagcaa tgttagaaag agaaaaagta atgactacat atatgggaat gggagttgct
     8281 attccacatg gtgtaaatga agcaaaaaaa gaaatattat cttctggaat agttatatta
     8341 caattcccta atggtataga ttttgatgga gaaaaagcat atttattaat aggaatagct
     8401 ggtgttggag atgaacattt agaaatacta tctaatatag cagtagtttt agatgatgac
     8461 ttaacagaaa gactaaaaaa ttcaaatgac aaacaagctt ttatggaagc ttttgccaac
     8521 taatcttagg agggatttaa aatgaaaaag gcaattcagt ttggagcagg aaatattgga
     8581 agaggtttta ttggaggctt gttagttaag tcgggttatc atgtagtatt tgctgatgta
     8641 aatgaggaga tattaaactc aataaacaaa gataaaaagt atactataca cataagagat
     8701 gtagaatgta tagatgaagt aattgataat ataagtgcgg tttcatctat aaaagaagaa
     8761 ataatagatg agattgtaca ggctgaaata ataactacag cagttggacc tttagttttg
     8821 actaaaatag cctctacaat tgcaaaaggt ataaaggcta gaaaagaaaa gggattaaca
     8881 agtaatctaa atataatagc atgtgaaaat gcaatatacg caagttcttc tttaaaagaa
     8941 gaggtactta agtatttaaa taaggaagaa gtagaatatt tagagatgta tgttggtttt
     9001 ccaaactgtt cagttgatag aatagttcct cctggtaaaa acgaaaatcc tcttgatgta
     9061 acagttgaaa atttttatga gtggaatgtt gaaaaacaag gatttaaagg ggaaatacca
     9121 actatagttg gtatgaattt agctgataat cttatggctt atatagaacg taaattgttt
     9181 acactgaata caggtcatgc tataacagct tatataggat atttaaaagg atataagact
     9241 atagaagaga gtataaaaga taaatttatc tgtgatatag tcaaaagtgc aatggtagaa
     9301 agtggagaag gattgattaa aaaatacaat tttgactcag aagtgcacta taaatatatt
     9361 gataaaattt taaatagatt taaaaatcca tatcttaatg atgatgtatt aagagtcgga
     9421 agggaaccat taagaaaact aagtgataaa gatagattga ttaaaccttt aatgacagca
     9481 aaatcatatg gattatcagt tgataatttg attcttggaa taggtgcagc gcttcattat
     9541 aataatagtg aagatactca aagtgtagaa ttacaagagt tgataaagtc tattggagta
     9601 aaaaaagccg ttgctaaaat agctaatata agcaatgacg aagaactttt aaacaatatc
     9661 gaaaaaagtt atatatttat gaaaaattta taaatttact tgaaaaaaag tagttctgat
//
LOCUS       DAYASV010000140         9571 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYASV010000140 DAYASV010000000
VERSION     DAYASV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.145"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            170..1210
                     /locus_tag="ACGI68_003168"
     CDS             170..1210
                     /locus_tag="ACGI68_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0139589.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1566..2888
                     /locus_tag="ACGI68_003169"
     CDS             1566..2888
                     /locus_tag="ACGI68_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFH0139590.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3185..4546
                     /locus_tag="ACGI68_003170"
     CDS             3185..4546
                     /locus_tag="ACGI68_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFH0139591.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4619..6013
                     /locus_tag="ACGI68_003171"
     CDS             4619..6013
                     /locus_tag="ACGI68_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139592.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYIHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6075..6545
                     /locus_tag="ACGI68_003172"
     CDS             6075..6545
                     /locus_tag="ACGI68_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HFH0139593.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6548..8764
                     /locus_tag="ACGI68_003173"
     CDS             6548..8764
                     /locus_tag="ACGI68_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HFH0139594.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8847..9518
                     /locus_tag="ACGI68_003174"
     CDS             8847..9518
                     /locus_tag="ACGI68_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0139595.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
BASE COUNT         3586 a         1129 c         1930 g         2926 t
ORIGIN      
        1 aaatgaagaa agtatatcta tcaaattaaa actaagtaga tatacttttt attaagtttt
       61 gttttaaaat aatattatta aattataact aagcttttct gttatgatat aattaaatag
      121 ataatgtgaa tgattattat atagaatact tatttggagt tgatatttta tggacaaaca
      181 cagtgattca gtaatgtatg gtggaatgga agtatataat atttggggaa caacaacagt
      241 agactccata attggagagt ctgatgtaat gcgtgctctt aagaatagaa tttgtaaaat
      301 tggaaatagt gattctactg tagctataac tggagaaagt ggcactggaa aagaattaat
      361 agcaagagcc attcatctcg caggaaacag agggaataaa acattcgttg ctataaactg
      421 tgctgctata ccagaacctt tactagaaag tgaacttttt ggatatacaa aaggagcttt
      481 tagtggagca gattcaaaag gtaaaattgg taagtttgaa ttggccaatg gaggggttgt
      541 atttttagat gagataggag atatgccaat tcaactacaa tcaaagttat tgagggtatt
      601 acaggaaaag aagtttacga gaataggttc aaatgaacta attgatatag atgttagaat
      661 catatcagct actaatagag atttatttga gttggttaag gaaaataaat ttagagaaga
      721 cttatactat agattgaatg taataccttt agaagtgcct ccacttagag aaagaggaga
      781 cgatatacaa atattaataa acaattttat agacaaatat tcgagaaaga tgaagaaaaa
      841 cgtgtaccat atagagccag atgtggaaca aatgttgctt aagtatcctt ggccaggaaa
      901 tattagagag ttggaaaata ctatagaact ggcattaaac cttcttgagg aagatggaat
      961 aatacataaa ggaataataa atagaaagat acttgaatat tttaagttaa ataatataaa
     1021 tataaagtta cttgaggaaa gtgactatga gataagtatg gaacaagaca tacttacact
     1081 tgaagagttt gaaagagcct atataaagaa agtgttaaag ttttacggaa atgatactaa
     1141 gtctaaaaaa ctagctgcca aaaaacttgg aatatcacta gctacattgt acagaaaaat
     1201 agatgtataa taattaggct ttttattctc aattctaaaa tttgaaatta taaaattgat
     1261 aaaaaacact tggaaaacta ttttctaagt gtttttttgt gtaatagcat tttattatcc
     1321 aaaaataaaa aaaatttaaa agaatttaaa aaaataaatg ctttaataaa ttatcaaata
     1381 ctaacaaata taataaaaat tatatatgta aaacaattat gttaaaaaat tggcattata
     1441 attgcttcat attaagttga gatgacaaaa taaaaaaaga aaggatgttg tgtgagataa
     1501 taataactgt cattaaaaaa tagttgggag tgataacctt gagtaaatat caaaagaatg
     1561 tttctatgtt tgatattgat ggaaggccac caattttaaa gtgtttgcca ctatcaatgc
     1621 aacatatact agcaatgata gttggaactg ttacagtacc tatagtcgta ggaggagctg
     1681 tgggtgttag ccctacacaa ataacacttt tagtgcaata tgcattaata atagctggta
     1741 tatcaacact tatacaacta tatccaatag gaaatattgg ttcaagattg cctataattt
     1801 ttggagttgg ttttacttat gtaccaacac ttgtagcagt cggaggaaat tatggattag
     1861 caagcatttt gggtgcacaa ttaatagggg gagtggctac agtattaata gggatattca
     1921 taaaaaagat aagaaaattt tttccgaata tagttgcagg aacagtggtt tttacaattg
     1981 gattatcttt gtatcctata gcaataaatt atatggcagg tggtgtaaat agtcctactt
     2041 atgcctctat gcaaaactgg gcagtagcgg ctataacatt agtggtagtt ataggtctta
     2101 gtcaatttgc aaaaggatat gcaaaattgg cagcaatatt tttgggaatt atagttggat
     2161 attgtgtatc tttagttctg ggtataataa attttgaccc tattagagag gcaacttggt
     2221 ttgcgatacc taaaccgctt gaatttggga tggaatttga tatgcctgta ataatctcag
     2281 tagtaattgt aagtataatc aatgctgttc aagcaattgg agatttatca gctactacaa
     2341 tgggaggcat ggataggaat atcacagata aggaattatc aagtggagta ataggaagtg
     2401 gaatttgtac tatgatagga gctttgtttg gaggtttacc accatcatca tatagccaaa
     2461 acgtggggat ggtagctatg aacaaagtaa taagtagatt tgtgcttgga atagctggta
     2521 tatttatgtt attgtcgggg tttgtgccta aatttggtgc actaatgact actataccat
     2581 attctgtttt aggaggagct acaatatcta tatttagtat gataacaatg acaggtataa
     2641 agttaattat tacagaagag ttgtcatcaa gaaatattac cattgttggt ttggcaattg
     2701 ccttaggcat gggaataaca tcagtaccag cagcccaaga ggcatttcct tcatttgtaa
     2761 agttagtatt tggagaatct ccaatagtaa ttgcaacatt ggttgcattc gttttaaatc
     2821 taataatacc aaataaaagt ctagaggatg aggctaaaga aagagctgtt atggaagaga
     2881 ctaattaaga tataataatt taaaagaatt gtttaaatta tttagtatga gattgttaga
     2941 ttatttttga tagctattca ttttaataaa aaatttaaca aataaatata taccaggcaa
     3001 ttaatgttca tttaagttga aaatcgggga gggaatattt agaaatttgt taatttttta
     3061 taaaaatagc tcagtttagc tgtgtttatc ggttgagcaa taaattcatg gtcataaaaa
     3121 tatttataga aaataattat aaaaatattt agtcaagata tttatgtatg ggaggattta
     3181 ttttatgaaa acacgtaata cttcaaaata tgatgtcgat gggataccac cactaaagga
     3241 atcaatacct ttggccttac aacatctgtt agcaatgata gtaggaaata tggtacctgc
     3301 gatattaata gcaaacgtgg taggacttaa ccaggggcaa gcaacaatgt tgattcaggg
     3361 ttctatgtta gcagcaggtt tagcaacgtt tttacagtta tatccaatac ctttatttaa
     3421 aggatttaag ttaggttcaa gattacctgt aatgatggga atgagttatg tatttttagg
     3481 agcttgtctt tcagtagcag ctaaagatgg tttagcagct ctatttggag cacagattgc
     3541 tgctggggta atcgtattct ttgtaggatt tggagttaag aaaattagac atatattcac
     3601 tccaatagtt tctggaacta taatagcatg tatgggactt ggactattcg caacagctat
     3661 aaagaattta gctggaggag aaggcacaca aacatttgga agcccaataa acttcacagt
     3721 aggagttata gttgcttttg taataataat gataaataaa tacggtaaag gtttagtaaa
     3781 aaattcttca atattaatag gaatattagt aggttatgct atatctttag ttttaggatt
     3841 ggttgatttc tcagccgttc aaggagcagc tatagtatct ttaccaacac cagcagcttt
     3901 tggattagaa tttagaccag agttaatagt aatgtttact attatttaca taataggtat
     3961 agctgatatg atgggtgcct gtacaatagc tacagtagga gcaatggata gagaggtaac
     4021 tgacgaagaa ttagcatcag ttgttttagg aaattctata acttctataa tttcatcact
     4081 atttgcagct cttccaactg gagtatttag ccaaaataca gtaattgtat ctatgaacaa
     4141 agttacaagt agatttgtaa tagcactagg ggcacttgta ttattactag cagggatatc
     4201 tccagcattg ggtgcaataa tgacaactat accatcttgt gttgttggtg gagcaacttt
     4261 agtagttttc tcatcaatag caatgtctgg tttctctatt atgagtatgg atggattcac
     4321 tgaagaaaac aatcttatag caggtgtatc tatagcaaca agtatgggtc ttacaactgc
     4381 tccacaagtt ttagaccaat tcccagaaac aattggaact gtccttggag ggtcaagtat
     4441 agtttctgga gcaataatag ctatattact tcaaacatta tttaaattaa agtcacgtaa
     4501 aagtgctgaa aacgttactt caaatcttga ggaaaatatt ggataaatgt attagatata
     4561 atatgagaaa accatatggt aaaatgttgt aatgaaagta ttcaaagggg gatttaagat
     4621 gattgatata ctaataaaaa atgcaataat tgttacagtt aataaagaca gagatgttat
     4681 atttgatgga gcaatagcta ttcaaggtga cagaatcttg gacataggaa atacagttga
     4741 tattgagcct aagtatcttg atgctaaaaa agttatagat gctaatggaa aagtaatatt
     4801 ccctggcttt ataaatactc ataatcacct gttccaagtg ttattaaaag gtttaggtga
     4861 tgatatggca cttcatgagt ggttaaatac aatgatgttt ccatcagcaa aattcttaac
     4921 agaacaagat acttatgatg ctgcaatgct tggatgtatg gaaggtttaa aaagtggtat
     4981 aactacaatg gttgattata tacatactca caatagacca ggacttacag atggaatagt
     5041 aaaggcatat aaagatttag gtataagagg ggttgtaggt agaggttgta tagatttagg
     5101 tattcacaaa gagcttatag aagatgtgga aacggttgaa aaagatttga gaagggtatt
     5161 cgaaaaatat cataattcag aaaatggaag aataaaaatc tgtgtagctc cttcttcaat
     5221 gtgggctata tctgaagaaa tgggtaagat gctttggaat atagttaaag aatatgattc
     5281 atattttaca gttcatatat ctgaaacaga atttgcaaga acagcaacaa aggatataca
     5341 tggagaaata gatataaaat tacttgaaaa atggggtata gtaggtccag aagttgtagc
     5401 agtacattgt gtatgtataa ctgatgaaga catggaaatg cttaaaaagt atgacataaa
     5461 agtttctcat aatgtagcaa gtaacatgta tcttgcttct ggtgttgcac ctgtgccaga
     5521 aatgttaaaa aaaggtataa atgtgagttt aggtcttgac ggtgcagcaa gtaataacgc
     5581 acaagatatg gtagagctta tgaaattaac agctttacaa cacaaagtaa ataattgtga
     5641 ccctctggca atttctgcag agaaagttct tgaaatggct acaattgagg gagcaagaac
     5701 tcttagaatg gaagatgaaa taggttcttt ggagattggg aaaaaagctg acctagtaat
     5761 atttaactca atgctttctc caaaatcaat acctcttcac aatcctgtat caactttggt
     5821 atactcagct agtatgcaca atgtagagag tgttttagtt gatggaaatg taatacttga
     5881 aaatggaaaa gtaactacaa ttgaaagtga agaacaagtg ttgcttaatg cacaacatgc
     5941 agcagaagaa ttatgtaaga gagctaatgt aactaataga atggaaggac ataagtggaa
     6001 tagcttatac taatattaaa ataagattgt aataatattc tattacaaca ttagtttatc
     6061 tgtaggagga gaaaatggaa aaaataaaat taaatattaa tgattacatg tatgaaatag
     6121 aagttgaaaa gaatgaaact cttttgcatg tacttcgtga aaaattaaat ctaactggag
     6181 cgaaacaagg ttgtaatagt ggcagttgtg gagcatgtaa agttttgata gatggagttg
     6241 acatgaaatc ttgtaaactt cttgcaaaaa actgcgaagg taaagttata tacactatag
     6301 aaagttttgc taatggagaa aatcttcatc caatacaaga agcttttata gaagtcggag
     6361 ctgtacaatg tggatattgt actcctggta tgattataac aacacaagct cttttaaata
     6421 gaaatgcatc tcctaatgaa gatgaaatta gagaagcttt taaagaaaat ctctgtagat
     6481 gcacaggata tgtaaagatt atagaagctg taaaattatc tgcagctaga ttagggggta
     6541 gataattatg gaaaaggtaa taggaaaatc ataccctcta aaagatgcag ctttaaaagt
     6601 tactggtcaa atgaaatacg tggctgatat gaaaccacaa aatgtacttt acgctaagat
     6661 gcttttaagc cctgtagcac atgcaaaaat aaaaagtata gatacaagtg aagctgagaa
     6721 agtagtaggt gttagagcta tagctactta tctcaataca tcacaaagaa aatacaatgg
     6781 ttcattaaga ttttatgagc acaaaatacc tgaaaatgaa gttatatttt cagatacagt
     6841 tagatttgtt ggtgaccgtg ttgcagcagt agctgcagag actccagaaa tagcagagaa
     6901 agctgtaaaa cttataaaag ttgaatatga agaattgcca gctgtattta ctatacaaga
     6961 tgcaataaaa gaagatgcta tctgcataca tggagattca aatatagttt ctcaaaacaa
     7021 cataaatggg ggagatgtag aaaaaggctt caaggaatgt gattatatat ttgaagatga
     7081 atactctact ccagcaatac atcatagtgc aatagagact catagtgtaa ttgcacacta
     7141 tgattataat gataaattaa ctgtacatac accttgccaa aatacatttg gatttagaat
     7201 aatactatct caaatttttg ggatgtcatt aaataaagta agagttataa gacctgctat
     7261 tggaggctca tttggtggaa aattagaact tacaattgaa ccagtagcag ccgcattatc
     7321 aaaaatgaca agaagaccag ttaaaatggt tcttacaaga aaggaatcta tgatatcaac
     7381 aagaactcgt catgggtctt ttgtaaaatt aaaaactggt gtaaataaag atggtgaagt
     7441 aatagcacaa gacataaaaa tatatacaaa tacaggagca tatgcatcaa gtgctttaaa
     7501 tgttatagga gcacttagtc ataaagtatt taaagtttat aaaataccaa atataaagtt
     7561 tactgggatg cctgtatata caaatacacc aatagcagga gctatgagag gctatgggtc
     7621 acctcaaata tttatggctc aacaagctca atttgcgaaa atagcaaagg aaataggtat
     7681 ggatttagtc gattttcaaa ataaaaatgc agtagagcca gatgatgtcg atataatctt
     7741 ccacggttct cttggaaatc ctagagtatt ggattgtata gagcaaggta agaagatgtt
     7801 taagtgggat gaaaagaaaa aacttccaaa agaagaagga aaatatttaa gaggtatagg
     7861 tatggcaata ggtgcacatg gtaatggagt ttttggtgca catagagatg ttgttacatt
     7921 gactcttaaa ttaaatgaag atggtacgtt gacactacta acaggagctc atgatatggg
     7981 taatggtgtt gtaactatgc aaacaatgat ggtagctgaa gttttaggaa taacacctga
     8041 taaagttgat acatttgaaa ctgatacgga tgcatgtaca ttcaaccttg gtgactatgc
     8101 aagtagaggg gtatttgtag aaggtggagg agctaaaaag acagcagaga aattgaaaaa
     8161 tcttatatta gaagaagcag aaaaattact tgaaacatca aaagaagatt tatatctaga
     8221 caatggatgt gtaattagta aacttgatga aaatgtaaaa gcatcacttt ctgatgtcgc
     8281 agtatatagt caaagtaaat cattaagaga gttaactgtt gttgaagatt attcttcacc
     8341 agctggacgt acttcttatg gtgtacattt tgctgaagtt ttagtagata aagaaactaa
     8401 ggatataaaa gtagttgatt ttgtagctgt acatgatgtt ggtagagtta taaatcctct
     8461 aaatttagaa ggacaattag aaggtgggat tcatatgggt ctaggatacg cattgagtga
     8521 agaattggca tttgatgaac aaggtagacc taaagctact gattttaaaa catataaatt
     8581 atttagaact gtagatatgc caaaatgtca agttgcattt gtagatggtt atgaaaaagc
     8641 tgggcctttt ggtggtaaga gtattggaga atgtgccgtt gtaccagttg cgccagctgt
     8701 tgcaaatgca gtatataatg caactgatgt agagatacat tcattaccaa taaaattaaa
     8761 atagtagaaa ttaaagcaga aataacaatt tattgtattt gtaaatagat aataaaaata
     8821 gatttatttg gtgaggagat attattatgg caaagatatt aggtatttgt ggaagtccac
     8881 gtaaaggagc aacagaatat gcagtgttag aagctttgaa agaagcagaa actatacctg
     8941 gtatagaaac tgagtattgg tcagttagag ggaaaaaaat aagtccttgt gtacattgtg
     9001 atgcatgtat aagaaaagaa actatgtgta ttataaaaga tgatatccaa gaattagagc
     9061 aaaaaatatt ggaggcagat ggaattatag taggttctcc agtttatgat atgaatataa
     9121 ctgctcaact tacagcagta tttaatagac ttcgtccaat gtatttagtt catccaggaa
     9181 aacttcaaaa taaggttggt gcagctataa ctactggtgg aactagatat ggtggccaag
     9241 aattgacaaa acttcctata ataaatttct tcctaatgca tgaaatgtta gtgtctggag
     9301 gtttaggtgg atgctatata ggtggtacaa tatggagtaa agatggaaaa gcaaaaggtg
     9361 cccaagagga tgagacaggt atggacacag taaaaagact tggaaaaggt gcagcagagg
     9421 cagttatggt ttctaagttt ggtcttgaaa aatggaatgt cgtaaaagaa gaattagatg
     9481 ttaaaaaaga tgaaaaatca cctcttagag accactaaag atttattata ttattgaata
     9541 tatacaaata gtgatgtaat agattattgc a
//
LOCUS       DAYASV010000141         9541 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYASV010000141 DAYASV010000000
VERSION     DAYASV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.146"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            162..809
                     /locus_tag="ACGI68_003175"
     CDS             162..809
                     /locus_tag="ACGI68_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFH0139596.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(976..1545)
                     /locus_tag="ACGI68_003176"
     CDS             complement(976..1545)
                     /locus_tag="ACGI68_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139597.1"
                     /translation="MKKFILCIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1550..2449)
                     /locus_tag="ACGI68_003177"
     CDS             complement(1550..2449)
                     /locus_tag="ACGI68_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0139598.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIIFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGIPLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2460..2609)
                     /locus_tag="ACGI68_003178"
     CDS             complement(2460..2609)
                     /locus_tag="ACGI68_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HFH0139599.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2602..3186)
                     /locus_tag="ACGI68_003179"
     CDS             complement(2602..3186)
                     /locus_tag="ACGI68_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HFH0139600.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
     gene            3575..4900
                     /gene="cdeA"
                     /locus_tag="ACGI68_003180"
     CDS             3575..4900
                     /gene="cdeA"
                     /locus_tag="ACGI68_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HFH0139601.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIFALVFA
                     PFIVRVFVSPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(4995..5789)
                     /locus_tag="ACGI68_003181"
     CDS             complement(4995..5789)
                     /locus_tag="ACGI68_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139602.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMIIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(5790..6665)
                     /locus_tag="ACGI68_003182"
     CDS             complement(5790..6665)
                     /locus_tag="ACGI68_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0139603.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLSFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSESDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(6669..7037)
                     /locus_tag="ACGI68_003183"
     CDS             complement(6669..7037)
                     /locus_tag="ACGI68_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0139604.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(7220..7879)
                     /gene="deoC"
                     /locus_tag="ACGI68_003184"
     CDS             complement(7220..7879)
                     /gene="deoC"
                     /locus_tag="ACGI68_003184"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFH0139605.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(7915..9102)
                     /gene="murJ"
                     /locus_tag="ACGI68_003185"
     CDS             complement(7915..9102)
                     /gene="murJ"
                     /locus_tag="ACGI68_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFH0139606.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMIIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(9179..>9541)
                     /locus_tag="ACGI68_003186"
     CDS             complement(9179..>9541)
                     /locus_tag="ACGI68_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0139607.1"
                     /translation="QKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFIR
                     QLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDIN
                     ASINILKEGLRLITIQNK"
BASE COUNT         3231 a         1534 c         1056 g         3720 t
ORIGIN      
        1 ttatactaat cgcatattta tgttataact atattgtaac tattttcgta atcttaggaa
       61 aagattactt ataaaattaa ccttatacaa taatatatcg tataatctta attagcataa
      121 tagttacaat attaaaattt aaatatggga gtgattttag tatgataaga caaacaccat
      181 tagagaaacg atatgacaag ctaggtcctc atattgtaac agcactcaaa aaacgttatt
      241 ttgatgctta ttactgtaaa actagaaatg aagctttgca acaaatttta gatttaattc
      301 ctcaaaatca tgtagtatca tggggaggat ctgaaacact taaagaaatg ggcgtacaaa
      361 ctgctgttag agagaaagga tttaaagtta ttgatagaga tttagcaaaa actccagaag
      421 aaagaactga aattatgcgt caagcacttc tatgcgatac ttttttgatg agtagcaatg
      481 caattagtga agatggacaa ctctttaata ttgatggaaa tggcaatcgt gtagctgcaa
      541 tgatatttgg tccaaaaaat gttatagttg tagctggtat gaataaaatt gtaaaaaaca
      601 ttgatgatgc aatacaaaga actagaacaa ttgcatctcc agcagttgtt cagcgttttc
      661 aagatgtcaa tacaccatgc tatattaatg gaagttgtat agattgcaca agtcctgaaa
      721 gcatttgtgc atacatggtt actacaagaa tcagtcgtcc agcaaaaaaa ataaaggtaa
      781 tattggtagg agaaaatctt ggtctataaa atatacatac atgtaaatag ctctataaat
      841 aataaaatca tatgaaataa cttatttcta aaagtggtag aataaaagct tctacaagac
      901 tttcattctg ccacttttat attcttttaa tttgtgtttc tatattatta attgctccaa
      961 ttttcatata tattactaac tatttttatt ttcataattt ttttgttctt catcaagctg
     1021 tttataataa tctttattat agtcagctag ataatctata ccacctcctg taatcacatt
     1081 taataatttc attgcagatt cttgattaac tttacaaaca taattatcac cagatttaac
     1141 aagctctaat gtaatattaa attctttttt ctttgcagaa tcaatatctt tcatagttgg
     1201 atttggcatc atttctccta gtttaggagg attctttttt aaaatagaaa ccatatcagt
     1261 attgttaatt tttaaatcta cagtcacttt atccttatta ttttttatat ctgaaatttc
     1321 ccaagtcata tttttagaat acattttaaa tccttcttta tcttcgtata taagattacc
     1381 taaccctgat tttgggaaat acttatcaac agtatctgca tcttgtgttt ttattgaatt
     1441 aaacatacta tccacttcat tttttatctt attatcatta gtacaaccag ctaacaccag
     1501 tactgatatt gcaaacatta taatacatag aataaatttc ttcattaaat tatatccccc
     1561 tatacttttt tgcacaaata ttattctctt ttttatcttt tgaaaaagtt gtagtaattg
     1621 cattttgtgg acaaactttt atacattcac cacacctaat acagtctgga ctatttacat
     1681 ctttatatat tggtatatca agcttacatt ttgtttgaca tgctgtacaa tttgtacatt
     1741 tatctttatc aattttaaat ctatatattg aaataggatt aaaaaaacca tagattgctc
     1801 ctaaagggca tatatacttg caaaatggcc tataagtaat gattgacatc aataatatca
     1861 ataacaaaat acttaacttc cagctaaata aaaatcctat tgattctctt aaattctcat
     1921 ttcctaaaat aagtggtatt cctcccaaaa gtgttcctga agggcaaatc catttacaaa
     1981 accaaggatt tccaccccct gctacattta caatagcaac tggcaaaata attacaaata
     2041 ttattaagac tatatacttt agatatttta gatatttatc accataaact ttttttatct
     2101 ttactggaaa gggtacttta tgtagtaaat cttgtacaag tccaaaggga caaagccaac
     2161 cacaaacaaa tctgccaaaa aaagcaccaa atgccattaa aaaaccaaca acataaaatg
     2221 agaatttata ctctctgctt gataaaactg cttgtaatga acctattgga catgctccaa
     2281 gtgcacctgg acatgaataa caattcaatc caggtacaca taatttttta ctttctcctc
     2341 catatatagt tccttctaaa aatccattta catatccatt tgttaatgct gtaaatgcaa
     2401 cttgagtaat aagtcttata ttattattta ctttagataa taacttcatt cctcttctcc
     2461 taaccaatac caatacactc aagacaaatc ataattgctt tagttaatac tgtatttgtt
     2521 tctccacgat atgtacctaa tgcaataaat ataatactaa ttgcaataat agaaatgcct
     2581 tttttattat ctttaataaa gttactcatt ggcttttacc atttttagtc tttcatctat
     2641 aatttttttc caatcttcct tactatttgc acctacaacc ttctcaccca caaagtttcc
     2701 tttcttgtct acaaaataag ttgttggaaa ctccataact cttttaaaat cagttttttt
     2761 aagttcttca gatacagtca attgttggta agttgcatct gactttttca taatgtcttt
     2821 aactaaattt ttctcttcat ttgaaagacc agttctcata tcagttttat caacttctac
     2881 aactaatccc tttatagcta cattggaatt attactctca tattcttttg ataatgcttc
     2941 aagctctggc atttcaccca cacatggtcc acaccaagta gtccagacat taataagagt
     3001 aagttcttta tttgaaaata tctcttttgt taccttattt ccatcaacat cctccgtatc
     3061 taaattacta aaccattcac tattattctt gtttttttct actggcttag attctttttt
     3121 gctacatcca gaaatcatac ctaaaacaca aatacatcct aaaactacta tgaaaaacct
     3181 tttcatatct cacactccta gaatttatat agtatataaa atcgctgctt gaaataaaaa
     3241 taacttatct tctatattaa agtaattata tttaataaat aagagctaat ttaaaaaaag
     3301 atatacagtt aaaatctatt tcttttcaga caaaatctta tatatatata atagcttgta
     3361 aatcttaaga ttatatacat atatttctaa agattttctt aagaaacagt tagattttac
     3421 taatataagt ttattaatta gcattatact aatttatctt aatatgattt tttgttgatt
     3481 ttaagctgtt tatcgtataa ataaaatttt tcttgactat atagttacta tatagcttat
     3541 tatatataga gagtaataaa aggaggtaaa atttatggaa aatttattta caagaaaatt
     3601 cactactttt gaatttctaa aatttgtttc tcctgcaatt atatccatga tatttatatc
     3661 tttgtacaca ataatagatg gcatctttgt atcgacatta gttggttctg atgctcttgc
     3721 tagtataaat attgtactac ctataattaa ccttgtttgt ggatttggaa taatgatggc
     3781 aactggtgga ggtgctatcg tttctatacg tatgggtgaa aatagacagg atgaagccaa
     3841 ctctacattt tcttttatag ttttgttttc attgattgtt gggattttat tcacagtaat
     3901 ctcatatttc tttatcaaag aaatatctat tttgcttggt gcaacagata agttattacc
     3961 atattgtata acttatggta aagttatgat tttatgtaca ccattttata ttttaaaatt
     4021 tatatttgag tactttgcaa gaactgatgg aaattctaaa tttagtttat ttctatcagt
     4081 cattggtggt gtaacaaata taatattgga ttatgtattt attaaatatt ttggaatggg
     4141 tcttttagga gctgcagttg caactgctat aggtattatt ttaacttgtg ttttaggtat
     4201 tatttacttc ttatctaata aatctacact aaaattaaga aaaccaaaaa ccgattttag
     4261 acttataaga gatactatga taaatggttc ttctgaaatg gttacagaat tatctacagg
     4321 aattacaaca ttcttattta atgtagtagc tttaaaatta gcaggagaaa atggacttgc
     4381 tgctcttact atagtattgt atgctcattt tttaatgaca tcagtctatc taggattcgc
     4441 tgctggagtg tctccattaa taagctataa ttttggtgct gaaaacagtg ataaattaaa
     4501 agaaacattt aaacattctc taaaatttat atttgtttct tctcttttag tgtttatttt
     4561 tgctttagta tttgcaccat ttattgttag ggtctttgta agtccagata acacagtatt
     4621 taaactagcc ttacaaggat taaaaatatt tgcatttgct tttttgtttg ttggtataaa
     4681 tatatttgca tcaggatttt ttacagcatt tcacaatgga aaaatttcag ctattatatc
     4741 ttttagtcgt gcctttgttt ttataatcat aggaatcata attcttcctc ctatgttaaa
     4801 catgactgga ttatggctta cagttccatt tgctgaggtt ataaccatat ttatatctat
     4861 tctatttata aaaaaatata aatgtagata taagtattaa aaattaatac ttaaaatcaa
     4921 catttattaa aactaataaa cctgctttaa aatacaaatt tacaaatttg atttttaagc
     4981 aggttttatt ttagttataa taagtcattt gcatactctt taattggtgc ttttctaagt
     5041 aataaaatta taaatattat gcaaataact ataattccta cttttgttcc aaataaaata
     5101 aaaggattct gaaatgaata aataattttt tctaaataca atagcatttt atgcatattt
     5161 ttagttgcaa aattaactat ataaattatc attaatatcg gtaatcctat caatatagta
     5221 atactagtaa cttttctaac tctatatgta aaagctccaa gtaacactcc tataaaacct
     5281 atacatattt catttataca tatttgtaca aaataattta aataagttag tggcatactt
     5341 gatgttgaag gatctatcag tgctgatata gatgtccaaa tcatatctct ctttagtaaa
     5401 actgatagtt ctatactgta acctgttata atttctataa ataacttcga taaaaaaatc
     5461 aaaactatac ctaaaataga aatcacaaag cttaaaataa ttaaatctat gacaatagct
     5521 tttatacaac tttttctatc tgctcttata cttaaagctc ctgaaaagtc tttatttgct
     5581 atatttactc catacacaaa tagcagtata gttatatatg ttaaaatagg attcacaatc
     5641 gtggataaat tagttttaga tgcttctcca aaaatcttga gaataaacac tggcaaaata
     5701 ttcataacaa ttcccacaat cataaatata attatataca atttagtatt tttattaaaa
     5761 aattttatat atggctttaa ttcattcaat tacaattcct ccttcttagt catgttaaca
     5821 aacatatctt gtagccctat gtatcctaca tcaatatttg aatttttaag tattttttca
     5881 tcactttcac taaaatcacc atagtaaaaa taagcaactg catttccaaa tgccttttta
     5941 ggttttatat tttttataca ttctaatttt tctaactctt ctttatttcc agtaatataa
     6001 tgagcttttt gttttatgac atcaatttct tcatctataa taatttttcc ctcatttaat
     6061 attattacat gctccagtaa atcatctatt tcatcaatca aatgagttga tattattatt
     6121 gtccttggat tttttatata actatctaaa attatgttat aaaactcttg tctatttact
     6181 gcatccagac caattgttgg ctcatcaaat atggtgatag ctgaattaga acaaatacct
     6241 attatatttg aaagtatagt tttcatacct cttgacagtt gtttatactt ttttttagta
     6301 tttaaatcaa aatatttgca taattcttct tctaagcctt tatcataact tttataaaaa
     6361 ctagaagaaa attcaaatat ctgacctact ttaaaatctg gactataaaa ttctttttcc
     6421 ctaacgatac aaacttcttc caaaactttt atatcttctt ttgggttctt tcctaatatt
     6481 tctatctcac cttcattttg aattaactga tttaccatta tatttaataa tgttgttttt
     6541 ccagctccat tcttacctaa taaaccatat atcttatttt tttcaaagct tagagacata
     6601 ttattaaaaa tattttgctt cttaaaactg tgagataaat tttctatctt aatagaacta
     6661 ctcatatatt aatcctcctt gaaattaatt ataatatcaa ccaattcttc tctatttatt
     6721 tctaatttgt tagcttcttg aagtaggttt tttacaaaat tatgtttaaa attttctttt
     6781 ctagcttctt ttataatagt ttttgcacca tctgatacaa acatccctat ccccctcttc
     6841 ttatataaaa tatttttatc aaccaatata tttatacctt ttaatactgt agctggatta
     6901 attttataaa gctttgaaag ctctgttgtt gatggaacct gttcttcttc ttttattcca
     6961 tttgatagta tctcatcttc aattgctcgt gctatctgaa taaatatagg ttcttcatta
     7021 tttaatacta atttcatagg ttcctccttt ctccatcagt tagttactca actaattaac
     7081 tatataactt ataatatctt gaaaaatatt ttctgtcaat actaaataaa aaacagaggg
     7141 aaaattccct ctgttttttg ttctttatat ttataattaa attttgattt ttgtaatcct
     7201 atcccataag ttaaaaactt taataatctg ttgtatcttc attttttaat attttacata
     7261 ttgagctagt tcctaacctt tctgctcctg cctctataaa tttttctgca tcagaaattg
     7321 atttaactcc ccctgcagct tttattttta cattttttcc aacatgctct ttcataagtt
     7381 ttatatcttc taaagtagct ccacctgttc ccattccagt agatgttttt ataaaatctg
     7441 aacctgacat tgttactatt tcacacattt ttattttttc atcttcatct aaataacatg
     7501 tctctataat gacttttaat actttatctc ctatagcttt ttttatttct tttatttctt
     7561 ttcctatatt atcataatct ttatttttta tatctgaaat gtttacaacc atatcaactt
     7621 catctgctcc attttcaata gcatcttttg cttcaaatac ttttgtagca gtagtttgat
     7681 atcccaaagg gaaccctatc actgtacata tctttatttt accttttaga tattctgatg
     7741 ctcttttcac atatgatggt ggtatgcaaa ctgatgctac actcatatct acagcttcat
     7801 cacataaaat tttaatatct tcccatgtag ttgtagcttt taaaatagta tggtctactg
     7861 tttttaatat atgtttcata aaatcctcct aaatttataa ttaatataat tagattactt
     7921 ttttaaatgc aattttctct ttgcaatgtt tagtatcata tcaacctctt caactttaaa
     7981 aataatcatt agtagtgtat aaattccacc tccaactata actgaaatag ctagtgatac
     8041 aaactcatta aatgttccta ctcccaaaat tccaaatata aatttatacg taaagtatga
     8101 taaaacaccc ataattaaag atgcaactac cgatttcaaa gttgctttta ttattttatc
     8161 ttgtccaaaa tatccaactt ttctctttag gttcaagaag agtaacaata tacatgcaat
     8221 tgatgatgaa ctagttgcaa gagcaagacc accatgagcc attggtttta ttaaaactaa
     8281 gtccaatact atatttaccc ctacagatat aatcccattt accataggtg tttttgtgtc
     8341 ttgtaatgaa taaaaaacct ttcccaaaat atctcttaag ccaaatgcag tcataccaac
     8401 tgcataaaaa attaaggcag ttgctgtcat ttgggttgcc ctcgcatcaa aagcacctct
     8461 ctcaaaaatg attctaacaa caggagttgc aaaaaaaata gaagcaactg aaataggaat
     8521 catagaaatt attatcatat ttatactgga tttaactgaa ctagtaaact tcttttgatt
     8581 attttctgca cttaacttag ataacatagg atacataaca gatactattg aagctgtaaa
     8641 tgtccctgta acaaaaccat ttaatctatc tgcataagta agtgctggaa tgcttccttt
     8701 aactaatgtt gaggctaaag ttgtatctac caatgagttc acttgattaa cagcaacacc
     8761 tataaatact ggtgaaagta atgctagtaa tttaataagg ctatcatctt taaagtttaa
     8821 gtagtataag tatttgtagt tcgttttttt aacaaaaaac atgtaaaaga gcagttgaac
     8881 taccatagca accacagcac caattggaag tatgtatggt ccaaatatgg tacttaacat
     8941 tattgaaata attattacta tattataggg tatacttcca aatccaacta caataaagtt
     9001 ctctttaatt tgtaaaaatg ctgacataac actcgtaata cctatgaata ttattcctgt
     9061 tctgaacctc ccatgactaa agtcacaggg ttcctgcttc atagaatttt gcatactaaa
     9121 atatgtctta ctaattctcc acaggctatc cccgtagttc ctacggttct tacatacgtt
     9181 atttattttg aattgttatt agtcttaatc cttcttttaa tatatttata cttgcattta
     9241 tatctctatc gtgagtttca ttacaactag gacaaatcca ttctcttata tttaagttct
     9301 taacttcctc gtttttatac ccacatttat tgcatatttg tgaacttgca aagaatttac
     9361 ctacctttac aatttgtcta ccataccaat tagccttgta ttctaattga cgaataaact
     9421 ctgaccatga tacatcagaa attaagcgag atagtttacg atttctaatc atattcttta
     9481 cttgtaaatc ttctatgcaa ataatatcgt tctcttttat taatttagta gatagttttt
     9541 g
//
LOCUS       DAYASV010000142         9387 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYASV010000142 DAYASV010000000
VERSION     DAYASV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.147"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            69..1829
                     /gene="prdR"
                     /locus_tag="ACGI68_003187"
     CDS             69..1829
                     /gene="prdR"
                     /locus_tag="ACGI68_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HFH0139608.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            2258..4138
                     /gene="prdA"
                     /locus_tag="ACGI68_003188"
     CDS             2258..4138
                     /gene="prdA"
                     /locus_tag="ACGI68_003188"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HFH0139609.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            4219..4503
                     /locus_tag="ACGI68_003189"
     CDS             4219..4503
                     /locus_tag="ACGI68_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBO2463/CBO2479 domain-containing protein"
                     /protein_id="HFH0139610.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            4541..5266
                     /gene="prdB"
                     /locus_tag="ACGI68_003190"
     CDS             4541..5266
                     /gene="prdB"
                     /locus_tag="ACGI68_003190"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050002 - D-proline reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:4991..4993,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HFH0139611.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            5346..6104
                     /gene="prdD"
                     /locus_tag="ACGI68_003191"
     CDS             5346..6104
                     /gene="prdD"
                     /locus_tag="ACGI68_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HFH0139612.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            6119..6586
                     /locus_tag="ACGI68_003192"
     CDS             6119..6586
                     /locus_tag="ACGI68_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0139613.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            6613..7086
                     /locus_tag="ACGI68_003193"
     CDS             6613..7086
                     /locus_tag="ACGI68_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HFH0139614.1"
                     /translation="MGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSEHNE
                     WKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIMGAD
                     GLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK"
     gene            7106..8113
                     /locus_tag="ACGI68_003194"
     CDS             7106..8113
                     /locus_tag="ACGI68_003194"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HFH0139615.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            8233..9114
                     /locus_tag="ACGI68_003195"
     CDS             8233..9114
                     /locus_tag="ACGI68_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFH0139616.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGAKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         3683 a         1154 c         1876 g         2674 t
ORIGIN      
        1 ttataatatg ctagaataaa tatatataaa taagttttga tgaggtacta ctataaaggg
       61 gggactttat gttttcgata ccagaagtaa aaaaagtaga agaggtaatg gatacaaaat
      121 ttacaactat agacgaagac acaagaattg aagatgcaat aaaagaaatg ataaaaagca
      181 acacaaaaac tctaatggta atagattcat ctgaccaatt aaaaggcatt atatccatga
      241 cggatataca taatctttat gagatgcata aaaagtatga aggtcaacca gtaaaactta
      301 taatgaagaa ggatgtaata tatgtcaacg aaggtttaac cttagatgaa tgtagagata
      361 taatgatatt aaaaaatata gggattcttc ctgttcttag agataataaa attattggag
      421 ttctaaaaca agagcacatt agggactatc tatatatgca tttagaagat tatggattaa
      481 ctttgaagta tattataggt caaattaaag aaggaatttg tgctataaat aacgaaggtg
      541 ttgttatctt gtggaataag tttatggagg aaaggtatga tataaagtct gaagatatag
      601 ttggtcgacc gatgaatgaa tttttggaaa atactatttc agaaaaagta ctaaatagta
      661 aagttggaat gagtgacttg tattttacag ataaaaaaga aaatatgtat gcattagttc
      721 atgcaaatcc tatattttat aaagaagaat ttatcggtgt tgtatgtaca gaagttgatg
      781 ttacagaagc taagatatta gctcttgaac ttgaaaaagt aaatgataca ttaaaatatc
      841 taaagaacga agtcaaaaat ttatctaaag gtagttttga taagatttta ggtaaaagtt
      901 ataaattgga aaaatcgaaa gcgattgcaa aacaagtggc tagaacaaat agtagcatat
      961 ttatttgggg cgaaagtggt acaggtaaag aagtatttgc aagagctatt catgattata
     1021 gtgagagaaa aggtcaattt atacctgtta actgtagtgc cataccaaat gagttatttg
     1081 aaagtgagtt ttttgggtat gaatcaggtg cgtttacagg agcaagtaaa aagggtcgaa
     1141 taggtatatt tgaacttgca aaagatggaa ctgtattttt agatgaaatt gccgatttac
     1201 ctcttagtat gcaagcaaag ttgcttagag tattacagga aaaagaaata agaagagttg
     1261 gtggagatac cacaataaaa ataaatccta gaataatatc agctaccaat aaagatttga
     1321 gtaaaatggt aaaggcagaa aaatttagag aagatttata ctatagatta aatgttgttg
     1381 aaataaagat tcctccactt agagagagaa aagaagacat aggattattg gttcatagct
     1441 ttttagagga gatatgtaaa caaaataata agcctgttct tacaattagc aaagatgtaa
     1501 tagatatatt tcagaattat agatggaaag gtaatattag agaattaaaa aatacgattg
     1561 aaaatattgt tgtattgtca caaaattcta aaatagatgt ggatgatgta ccaagttata
     1621 tgatggattc tacaaataat agtactgaag aagaagagta tcctttagat ttaacgaagg
     1681 caactcaaaa aattgaaata aagaatataa ccaaggcact taaaatgtct aatggaaata
     1741 aagctaaagc agctaagata ttaaatatac ccagaacaac tttgtattat aagatagacc
     1801 aatacaaaat agatgtgtct aaaatatgac acatgactaa attttgacaa aatgataaac
     1861 tttaatttaa aacctagtaa agccaacgct tatataagtg ttgtgtatat agatgtgtta
     1921 aaaaaaatga aaaagtgttg attaattgac actttttttg tttgagaaaa tgtttacaaa
     1981 tacaaaaaat aacatatttt aaataaatat ttatataata tttggctaaa aatagcgata
     2041 gatgaagaat ctattataga aaaaatcttt ttgttaataa gttggcatag gaattgctta
     2101 taatataaag tgactttaag tgagaaaaaa ataatattaa tcataacaaa ttatgtaggt
     2161 tttaatttac ttaatttctc agttaagggg ttacctgttg aggaattgag atttaaaata
     2221 ataaataaga aaataatgta aggaggtatt ttatactatg tcaataactt tagaaacagc
     2281 tcaagcccat gcaaatgacc cagcagtttg ttgttgtaga tttgaagcgg gaacaattat
     2341 agcgccagaa aacttagaag atccagcaat atttgcagac ttagaggatt ctggattatt
     2401 aacaatacca gaaaatggat taactatagg tcaagtacta ggagctaagt taaaagaaac
     2461 tttagatgca ctttctccaa tgactacaga taacgtagaa ggatacaaag caggagaggc
     2521 taaagaagaa gtagtagaag aaacagtaga agaagcagct ccagtatcag aagcagcagt
     2581 agttccagta agcacaggag ttgcaggtga aacagttaaa atacacatag gtgaaggtaa
     2641 gaacataagc ttagagatac ctttatcagt agctggtcaa gcaggagttg ctgctccagt
     2701 agcaaacgtt gctgctccag tggcaagtgc agcagcagaa gtagctccaa aagttgaaga
     2761 aaagaaactt ttaagaagct taactaaaaa acactttaaa atagataaag ttgaatttgc
     2821 tgatgaaact aaaatagaag gaactacttt atacatcaga aacgcagaag aaatatgtaa
     2881 agaagctaat gaaactcaag agttagttgt agatatgaag ttagaaataa taactcctga
     2941 taaatatgaa acttacagtg aagctgtatt agatatacaa ccaatcgcta ctaaagaaga
     3001 aggcgaatta ggttcaggta taactagagt tatagatgga gctgtaatgg tattaactgg
     3061 tacagatgaa gatggagttc aaataggtga atttggttct tcagaaggtg agttaaatac
     3121 tactataatg tggggtagac caggtgctgc tgacaaaggt gaaatattca tcaaaggtca
     3181 agtaacaata aaagcaggaa ctaacatgga aagaccagga cctttagctg ctcaccgtgc
     3241 atttgactat gtaactcaag aaataagaga agcattaaag aaagttgaca actctttagt
     3301 agttgatgaa gaagtaatag agcaatacag aagagaaggt aaaaagaaag ttgttgttat
     3361 aaaagaaata atgggacaag gtgcaatgca tgataaccta atattaccag ttgagccagt
     3421 tggtacatta ggagctcaac caaacgttga cttaggaaac atgccagttg tattatctcc
     3481 acttgaagta ttagatggtg gtatccatgc attaacttgt ataggacctg catcaaaaga
     3541 aatgtcaaga cattactgga gagagccatt agtaataaga gctatggaag acgaagaaat
     3601 agatttagta ggtgttgtat ttgttggttc tccacaagta aatgctgaga aattctatgt
     3661 atctaagaga ttaggtatgt tagttgaagc tatggaagtt gatggagctg tagtaactac
     3721 tgaaggtttc ggaaacaacc atatagattt cgcatctcac atagagcaaa taggtatgag
     3781 aggtatacca gtagttggtg taagtttctc agctgttcaa ggtgctctag ttgttggtaa
     3841 taaatacatg actcacatgg tagacaacaa taagtctaag caaggtatag agaatgaaat
     3901 attatctaac aacactttag ctccagaaga tgctgttaga ataatggcta tgcttaaaaa
     3961 tgctatagaa ggtgtagaag ttaaagctcc tgaaagaaaa tggaatccaa atgttaaatt
     4021 aaataacata gaagctatag aaaaagttac aggagaaaaa atagtattag aagagaatga
     4081 gcaatctcta ccaatgagta agaagagaag agaaatatac gaaaaagacg aaaactaaaa
     4141 tttagatact aactaagatt tagacaataa cataaattta gatccaagtt taaaacagta
     4201 tagagaggta gaattataat ggataggtta aaatatatct catcagagac tttttatgaa
     4261 ggtattatag ttgacatcaa aggcggtgga gtgactatag acctaaaagg tagacttgga
     4321 caatttaaaa tacctaatag aatgcttata actgactacg aacttaagat aggtcaagaa
     4381 gttggattca tgctaagcta tcctgaggta ttaagtccag agcctaatca agagtatgta
     4441 gaaaatatta ggagagaaaa agaaaaacaa gcagaaatgc aatcaaaaca agaagaaaaa
     4501 taataaataa tcgtttgaaa atagaaggga gaggaaatat atgagcctta caacagtaca
     4561 aggacttcaa tctgaaatat tcgttccaat aacacctcct ccagtttgga ctcctgtaac
     4621 taaagaatta aaagatatga ctatagcttt agctacagca gctggtgttc atttaaaatc
     4681 tgacaagaga tttaatctag caggagatac tacatttaga gctataccta atacagctac
     4741 agttgatgaa atgatggtat cacatggtgg atatgacaat ggagatgtaa acaaagatat
     4801 aaactgtatg ttccctatag atagattaca tgaattagct gcagaaggat ttatcaaagg
     4861 agtagctcct atgcactatg cattcatggg tggtggagga aaccaacatg tcttcactga
     4921 agaaactggt cctgctatcg ctgctaaact taaggaagag ggagtagacg gtgtagttat
     4981 gacagctggc tgaggtactt gccatagaac tgccgtgatc gtgcagagag caatagagga
     5041 agctggaata cctacaataa taatagcagc tcttcctcca gtagttagac aaaacggaac
     5101 tcctagagca gttgctccat tagttccaat gggtgctaat gctggtgaac cacataatat
     5161 agaaatgcaa acacatatat taagagatac attagagcaa ttagttgcaa taccatctgc
     5221 tggtaagata gttccattac catacgaata taaagctcac gtttaaaatg taatttatat
     5281 ttgaaaatat agaacccttc tcctacacgg ttaggagagg ggttttaaga acaaaggtgg
     5341 atattatgga agaaaaaata atgagaagac ttgttataaa accgtttcat atgaatgaag
     5401 ttaatttcgg ttcaaaaact tctataaaga aagatgtact tacaatagat ttatcaagta
     5461 tagatgaaat aaaagaaaga gaagagttaa taacagacat aaaagtagac attataaaac
     5521 ctggagatta tgacagagaa attaatacta ttatggacat tataccaata tcaacaaaag
     5581 ttttaggcag actgggagag ggtattacac atactttaac gggtgtatat gtaatgttaa
     5641 ctggtgcaga tgaagatgga aatcaaatgc atgaatttgg ttcttcagaa ggaattttga
     5701 aagaccaaat ggtatttggg agatatggaa ctccatcagt tgaagattat ataatccatg
     5761 tagatgtaac tttaaaggga ggtttacctt ttgaaagaac acttccactt gcagcattca
     5821 gagcatgtga tgattttata caagaaataa gagcgtcttt gaaaatggaa gacggaagaa
     5881 atgctactca agttcgtgaa tattttgaca aaattcgacc aaatgcaaaa aaggttgtca
     5941 tagtaaaaca aatagcaggt caaggtgcta tgtatgacaa tcagttattc tcaaaggaac
     6001 ctagtggatt tgaaggagga acatcaatta tagatatggg aaatgtacct atgattatat
     6061 ctcctaatga atacagagat ggtgccctta gagctatgac ttaagagagg tggatattat
     6121 gggaatagga ccttcaacaa aagaaacatc tcttcatcac tttagagacc cacttttaga
     6181 catagttgaa agtgataagg atgtagattt acttggagta atagtagtag gaactccaga
     6241 tggaaatgaa aataaaacct ttgtgggaca aagaacggcc gcttggctgg aagccatgag
     6301 agtagatgga gctatagttt catcagatgg ttggggaaac tcacatgttg actacgctaa
     6361 cacatttgag gaaataggaa aaagagatat acctgtagtt ggagttacat ttaatgggac
     6421 tcaagctaag tttgttgtaa gcaatcaata tatggatacc atagttgata tgaacaaatc
     6481 aaaagaaggt attgagacag aagtagttgg agaaaacaac acaaatgaaa tagatgctaa
     6541 aaaagctttg gcatttttaa aacttaaaat gagaaaacat ggataaaaaa tttaagtaag
     6601 ggaatgagtt ttgtgggaat ttctacatca atgaaaaaca catcacttca tatatttaga
     6661 gacccgttac tagatttgat agataatgat tctgacatag acttgttagg agtggttgta
     6721 gttgggacat cagagcacaa tgaatggaaa acatttttag caactagact tggaagatgg
     6781 attgaatcat tgcgacctga tggagtgatt ataactttag atatggcagg aaaccagcat
     6841 attgatttta caaatgctat agcagagttt gtaaaatcag atatacctac agtaggtctt
     6901 actattatgg gagcagatgg attagtaatt accaatccat atttggataa agctaccata
     6961 attgattata aaaagacaac aggctacata gaaacagaag tagttggaga caatcacatg
     7021 gatgaagttg atgttaaaaa agcattggct ttcctaaaat taaaaatgcg taaagacgca
     7081 aaataaacct aagggggaat ttgatatgaa atttagcaga agtatacaag ctatagactc
     7141 tcatacagca ggtgaagcga ctagaatagt tgtaggtgga atacctaaca taaaaggaaa
     7201 ttctatgcct gagaaaaaag aatatttaga ggaaaatcta gattatttaa gaactgctat
     7261 aatgttagag ccaagaggac ataatgatat gtttggttct gtaatgactc aaccttgttg
     7321 cccagatgct gactttggaa taatcttcat ggatggtggc ggatacctta acatgtgtgg
     7381 ccatggtaca ataggagcta tgacagcagc tatagaaaca ggtgtagttc cagcagtaga
     7441 gcctgtaact catgtagtta tggaagctcc agcaggaata ataagaggag acgttacagt
     7501 tgtagatgga aaagctaaag aagtatcatt cttaaacgta ccagctttct tatataaaga
     7561 aggtgttgaa gttgacttac caggtgttgg aactgttaaa tttgacatat catttggtgg
     7621 aagcttcttt gctataatac atgcaagtca attaggttta aaaatcgaac ctcaaaatgc
     7681 tggtaaatta actgaactag ctatgaaact tagagatata ataaatgaga aaatagaaat
     7741 acaacatcca actttagctc atataaaaac tgtagactta gttgaaatat atgatgagcc
     7801 aactcatcca gaagctactt acaaaaatgt agttatattt ggtcaaggtc aagttgacag
     7861 atctccatgt ggaactggaa caagtgctaa attagctact ttacatgcta aaggtgaatt
     7921 aaaagtagga gaaaaattcg tatatgaaag tatattagga actttattca aaggtgaaat
     7981 agttgaagaa actaaagttg cagatttcaa tgctgtagta cctaaaataa ctggttctgc
     8041 ttatataact ggatttaatc attttgtaat agatgaagaa gacccactta aacatggatt
     8101 tattcttaaa taagtaataa ataaattagg aaattaaaat agaatagtag ccacttgtcg
     8161 gttgctattc tatagctttt aggctataag attcagaata tatgaaaaaa taaaaaaata
     8221 ggaggatgga ttatggtagc aatcttaaag gcattatatg tagtattcgc agcaatcttt
     8281 gcagttgtgt ttggtaaaga cgtagctaaa gcaaacaaag aggggaaact agaagaacaa
     8341 agtactccaa aagttgctgt aacaggattt ataacagact tctttgatac attaggaata
     8401 gggtcatttg ctccaacaat agcaatgtca aaggctttaa agttaaatat accagataaa
     8461 aaaatgcctg gtacattaaa cgtagcacat actattccag ttgttacaga agcattcata
     8521 tttacatcaa ttatcccagt agatggtgtt actttagtat ctatggttgt ggcagcagca
     8581 gttggttcat atataggtgc aggaataata gctaagatgg acgagagaaa aatacaactt
     8641 gttatgggtg taactttagc attaacagca atattaatgt tattaggtca tccttggatt
     8701 aacgtattac caggtggagg aaatgaatta ggtcttactg gtgctaaatt agtaataggt
     8761 gtaataggta actttatact aggagcttta atgacagctg gtataggtct atatgctcca
     8821 ggtatggcaa tggtttattt cttaggaatg tctgcaaaag ttgcattccc aatagttatg
     8881 ggttcttgtg cattattaat gccagttgca agtatgaaat tcataaaaga agatgcatat
     8941 actaaaaaag catcagttat aatagctata tgtggtttaa taggagtttt cgtagctgca
     9001 tatatagtaa aaactctacc aatggatata ttaaaagcat tagtaatagt agttatagct
     9061 tatacttctg ctacaatgat aatggctgca aataaaaaca aaaagactgc ttagttaaag
     9121 actacttagt gtttattaaa tattacagct aataataaag attataaact gctttaaaat
     9181 attgaattaa tattttaggg cagtttttta tttactaaat taatataaag caattaacat
     9241 gccaattatg taatatatat agtgatttag tagatttaat ctaaaaaacg ttgtattttt
     9301 aaagaataaa gcataattta aagctaaata tttaaatttt ataatcaata agttcaataa
     9361 atgttcatat attaatacag attaata
//
LOCUS       DAYASV010000143         9333 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYASV010000143 DAYASV010000000
VERSION     DAYASV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.148"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(56..1873)
                     /gene="ftsH"
                     /locus_tag="ACGI68_003196"
     CDS             complement(56..1873)
                     /gene="ftsH"
                     /locus_tag="ACGI68_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFH0139617.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            complement(1904..2347)
                     /locus_tag="ACGI68_003197"
     CDS             complement(1904..2347)
                     /locus_tag="ACGI68_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFH0139618.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            complement(2838..3728)
                     /locus_tag="ACGI68_003198"
     CDS             complement(2838..3728)
                     /locus_tag="ACGI68_003198"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFH0139619.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            complement(3758..5023)
                     /locus_tag="ACGI68_003199"
     CDS             complement(3758..5023)
                     /locus_tag="ACGI68_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HFH0139620.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     regulatory      complement(5133..5235)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5289..7976)
                     /locus_tag="ACGI68_003200"
     CDS             complement(5289..7976)
                     /locus_tag="ACGI68_003200"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HFH0139621.1"
                     /translation="MKKRKEWSSKMENHKQMPWHSRATHEILESLDSSEEGLTDTEAE
                     KRLKENGLNELRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIV
                     TVNAVIGIVQEKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAM
                     VPADLRLIETSSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGR
                     GVGVVVATGMDTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGA
                     FYQRPLLPMFMIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGG
                     ATVICCDKTGTLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCN
                     DACINPDKTDEILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVH
                     NIDEKIIAYTKGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGF
                     AKREISSIPKEDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHK
                     VTATTIAHELGIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSL
                     KRIGEVPAMTGDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIK
                     EGRRVYRNIQKVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALA
                     LGVDPPSRNIMKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQ
                     TMAFCVLAFSQMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRN
                     AFNLTVLNNLEWLVTIVLAIMSVIQVEVGKIIKRKKLKK"
     gene            complement(8074..8817)
                     /gene="yaaA"
                     /locus_tag="ACGI68_003201"
     CDS             complement(8074..8817)
                     /gene="yaaA"
                     /locus_tag="ACGI68_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFH0139622.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            complement(8827..9291)
                     /gene="bcp"
                     /locus_tag="ACGI68_003202"
     CDS             complement(8827..9291)
                     /gene="bcp"
                     /locus_tag="ACGI68_003202"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFH0139623.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
BASE COUNT         2868 a         1734 c         1109 g         3622 t
ORIGIN      
        1 agttgtaaaa taaaaatgcc taacaatagg catttttatc ttgtgtataa tcattttagt
       61 ttaagttaag tatttccata aactcatcac ctgtaatagt ttctttttct aataaataat
      121 tagcaagttc atgcaattta tcaatattat tttctaagat ttcaattgca tttttgtgac
      181 aagcctttac tattttaaat acctcttcat ctattttagc agcagtgtcg gaagaacaag
      241 caagagaagt atctcctcct aaatattgat tgcttacagt ttcaagagcg accatatcaa
      301 actcttcact cataccaagg cgagtaacca tagctcttgc aattttagta gcttgttcta
      361 tatcatttga tgctcctgat gtacaagtgt taaagataac ctcttcagca gcacgaccac
      421 ctgtaaaagt agttatttta tcaattgctt cttctttaga cataagtaca ctctcatctt
      481 cagcaacttg catagtatat cccaatgctc ctgaagttct aggtattata gtaatcttat
      541 ggacaggtgc agaatgtttt tgcatagcag caacaagtgc atgacctatt tcatgatatg
      601 ctataatttt cttttcctta tctgaaatta cagctccttt acgttgatac ccagcaatta
      661 cagtttcaac agattcatca aaatcctgtt gttctactat atctctgcca gacttaactg
      721 ctaaaagtgc tgcttcgttt acaatatttg caagttctgc acctgatgct cctgatgtag
      781 accttgcaat tgcattataa tctatatctg agcttacatt tacattttga gcatgtacct
      841 ttaatatagc ttcacgtcct ccaaggtctg gaagttcaac tggtatacgt ctatcaaatc
      901 ttcctggacg aagtaatgct ttgtcaagac tgtcaggtct atttgtagca gcaagtatta
      961 ctactccaat tccaccatca aaaccatcca tctcacttaa caactgattt aaagtttgtt
     1021 cacgttcatc atttccacct aatccacttc cattatcacg ctttttacca atagtatcaa
     1081 tctcatctat aaacactata catggagctt tttcttttgc ttgtttaaat aaatcacgaa
     1141 tcctagaagc tcccattcct acaaacatct caacaaactc agaaccagat attgagaaaa
     1201 atggtacatt tgcttcacct gctacagctt ttgcaaggag tgtttttcct gtacctggag
     1261 gtcctactaa aagtgctcct ttaggcattt gtgctcctat ctttttatac ttttctggat
     1321 tgtgtaagaa atctactatt tcagttaaag cttcttttgc ctcatcttga ccagctacat
     1381 cattaaatct ttttccactt tgagcagata catacatttt ggcgttgctt tttccaaatt
     1441 gcatagcatt accacccatt cgcttttgca tagacttcat cattaagtta ccaaatagca
     1501 taagaatacc tataggtatt atccatgtaa gtaaaatact taaaagaggt gaattttcct
     1561 taggaataac cttagtaaat tctacttttg cattttttag tctatctacc aattctgggt
     1621 catccattct accagttatg tatatattct tatccttgct actagatgga tttattgcta
     1681 ttctattatt ttctatctca acagatttaa cctttcctgt atccaacatt gtgagaaact
     1741 ttccatagtc aatctcaacc atttttttat ccattaaact tggaaatatt aaagcattta
     1801 aaaacattac tactattatt ataactgtat aataatagag tgctgacctt ttttggggtt
     1861 tatgttcatc cataatttta ttctccttta aattattttt atgttatttg caatcaatgt
     1921 aagttaaaag tttatttaat ttctctaaac cttctattat atcttccata tcttgcaatt
     1981 tttcactggt catattttca aaataactat caaaaatact acttgaaata tgttcttgta
     2041 tatccaatga tttatttgta acttttactt ttacaattcg ttggtcttct atatctctta
     2101 tgcgatttaa aaagccattt ttttcaagcc tttgacagat tactgataca ttactatttg
     2161 tcatgtttaa gttcttccct aaatcgctta tttttttctc tccatgttca cataactcag
     2221 ataaaatcaa agcttgcact gctgtaatac caagtggctg ataaaaatca cttaataaag
     2281 ttgtaaattt catatttata tctctagtca actctagtag ctctttctta aaactcatct
     2341 catccataca aacgctccct acgaaaatac tttatttcaa atttaggctg aagtgcatac
     2401 tgtaaataaa tacttaacac tataatgttt aatgtattaa atattatagt attaagtatt
     2461 tattgtcaat tacaatattg taacttcaat tgaaattttg ttatcttatt gatatttaat
     2521 tttatacagg atatttttaa taaaatttaa taaaaaatct atatatatat tttacactga
     2581 tttctccctt tttaatctct tttaaaagtt aaaatacaaa tttattgata ctataaaggt
     2641 ttagtctccc attatcgcac catatataaa attaaagcca cctctatcta catacaccta
     2701 aagtgatttt cttaaaatat aaaactacta ttataaggtc aaattttaaa aaaataaggt
     2761 ataaagtatc attaagatta actttatacc tataaatttc tatatttttt tagttaacat
     2821 ttatatactc ttaaaattta ttgtatcaaa tagtttttta tccaatttct aaacaacaat
     2881 tctgaatgtt ttttccattt tacagttggc tcttctgaag ggtcatcatt aatatagtaa
     2941 ttttgaggta tagatatttt atctccttta tttatatccc tcttatattc tttatcaaga
     3001 gtgtatttgt catactcgct atgacccatt acgaacacat ctcttgaatt tgtaattata
     3061 tagggaccag cttctttaga atgactcaca atgctcaact ctttattctc tttcaaagct
     3121 tcaatattta cagtagtatg tcttgaatgt ggagcaaaaa attcattttc aaatccatct
     3181 actattttac tatctttatc taccttatgc ttaaatactc caaaacattt ttgacttagt
     3241 ggaagttttt ttacattgta gtatttgtaa agacttgctt gagctgccca acatatatat
     3301 aatgtagatt tagtttttct cttagaataa tccataattt cttctagttc attccaataa
     3361 tcaacttctt caaactccat ttgttcaact ggagctcctg ttattataaa gccatcaaaa
     3421 tactctcctt tggcttcatc aaatgtttta taaaattctt taacatattc attacaactt
     3481 cttttacttt ctcttgttga aattttaata aattccacag acacgttgaa tcctgtttta
     3541 tctatccttc ttagtaaatc taattctgta tctatcttta taggcattag atttagtata
     3601 gctatcttag tgtctatgtt ttcttcatac tttaattcct ttttaaaatc ttctttataa
     3661 attacatcta ttccttcatc taataattta ttaataacag gtaatccctt tggtagtatt
     3721 aaagccatat cttttcactt ctctttctct tagaatatta gaaatttaaa gcttgattta
     3781 aatcagctat taaatcctct acattttcta ttccaacaga taatcttatt agtgaaggca
     3841 atactccaga tgcaattaat tgttcttctg ttaattgtct atgagtagtt gaagctggat
     3901 gtataacaca agttcttaca tctgaaacat gtgtaaccaa agatgctatc tgtaattttt
     3961 ccacaaattt tttggcattt tctaacccac ctcttactcc aaatgataaa acaccactac
     4021 atcctcttga taaatacttt ttggcattct cataatactt gttatcttca agtcctgggt
     4081 aattaatcca atctacattt tcatgttttt ctaaaaatct agctatttta agtgcatttt
     4141 cactatgtct ctccattcta agatgtagtg tttcaacatt taaattagta agatacgcat
     4201 tgaatgggct taaacaattt ccatagtctc taagcaactg aactcttgct ttagttgcat
     4261 atgcggcatt tccaaatttt tgagtatagc ttataccatg atatgttggg tctggctcaa
     4321 ctaattctgg aaattttcca ttatcccagt taaagtttcc accatccact ataattcctc
     4381 caacacttga agcatgccca tctaaatatt ttgtggtaga atgagtaact atatttgctc
     4441 catactttaa agggttacaa agcactggag atgctaatga attatcaaca ataaatggaa
     4501 cattaattct cttagctaca tttgctattt tttcaaaatc tatgacttca agagttggat
     4561 ttgcaagtgt ttctgcaaac acaaccttag tattttcctt tgcaagttct actatctcat
     4621 cttcacttga atctaagtca aacgatatta aatctatacc aaatttttta agactaggtc
     4681 ctagtaaatt gaatgttcct ccatatactt ttgaagaaca aagtatacta tctcctgatt
     4741 tacatatatt taaaactgcc aacatatttg cagattgccc tgatgataca gctacagaag
     4801 atactccacc ctctagtaaa cttatttttt cttcaaaagc ttgaatagta ggattgctta
     4861 ttcttgaata tatatgtcca tcaactttta aatcaaataa ttcagcaagt tggtctatac
     4921 tgctatattt aaaagttgta ctttgatata aaggtagtac tcttggttct ccattacctg
     4981 gtttataatt tccttgcaca catattgttt ctttattata cattcttgta tcccccttag
     5041 attttagtaa aaaaaattaa actatttacc agatgagagt taaaataaat aaagcctgag
     5101 tatataactc aggctctgta taagcttaat tatactcatc tctcagcttt cgccgcagga
     5161 tttagcacca atgtatataa atatactggt tgccgggttt catagggcca gtccctccac
     5221 ctctctggat aagatttatt taatttttct atgttttata tcttatatat ttttcctaat
     5281 tttgtcaact atttttttaa tttttttctt ttaataattt tcccaacctc tacttgaatt
     5341 acagacatta tggctaaaac aatcgttaca agccactcta agttatttaa aactgttaag
     5401 ttaaaagcat ttctaaaaac aggtacaaat agtattactg ctattaaaaa tgcagaagtt
     5461 aaaaatgaga ttaaaagaaa tggattttta ccattatttc tattaaacat tgaatctgta
     5521 tttgaacgct gactaaatgc tcgtaacatc tgtgaaaatg ctaatacaca aaatgccatt
     5581 gtttgaccta cagaatgatt ttctatatta agaccaattt tatatgctaa aatagtcatt
     5641 attgccacaa atataccttg ggtaacaact ctttgtataa gatttttttc aaacaaagtg
     5701 gttgatttca ctggctggtg cttcattata tttctgcttg gtgggtcaac tccaagagca
     5761 agggctggaa gcgtagcagt tgcaaggttt acccatagaa tatgaacagc aagaagcgga
     5821 gtatcccaat ttaataatat tgctacaaac aacgtaagta tctctgctat attaccaaca
     5881 agcaaaaatt gtataacttt ttgtatgttt ctatatacac gtctaccttc ttttattgca
     5941 taagcaattg ttgtaaaact atcgtccatt aaaatcatgt cagaagaatc ttttgcaaca
     6001 tctgtaccac ttatacccat ggctatacca atatcagcag actttaaggc aggagagtca
     6061 tttacaccat ccccagtcat tgctggtact tctccaattc tttttagaga ttgtataatt
     6121 ctaagtttat caaatgggga tacacgagca aatactgtag tatttttaac agcttcatct
     6181 agttcatcat cactcaaatt atcaagttca tcaccagata tcacagtatt ttcttcactc
     6241 catattccta actcatgagc gattgttgtg gcagtaactt tatggtcacc tgttatcatt
     6301 atggttctta tacctgcttc tttacaagta cttactgcat caattacctc agttcttggt
     6361 gggtcaatca taccaaccat acctataaat gttaaatcat attctatatt ttcactatct
     6421 tcttttggta tagatgaaat ctcacgctta gcaaaaccaa gtacacgcaa tgcatcttta
     6481 gacatgttaa gacataattc gtgtatattc tttttatcca cttcagtaat gtttctttca
     6541 ccttttgaag ttaatatttt agtacatagg tcaagaattt cgtctactgc tcctttagta
     6601 taagcaataa tcttttcatc tatattatga acagttgtca tacattttct atctgaatca
     6661 aaaggctgtt caaatacacg tggatattct tcttcaagag cttcttgatc tattccaaat
     6721 tttttggcca taaaaattaa tgcgccctct gttgggtcac ctaatatttc atctgtttta
     6781 tctggattaa tacatgcatc attacaaagt gcaccagcat aaacaatctc tttatacact
     6841 aatggatgtt tttcagatgc agtatctatt gatgtcactt ttccagattc aaaatctcca
     6901 ttgacagcaa tatgtgtaac agtcatttta tttaaggtta atgtaccagt tttatcacaa
     6961 catattactg tagctcctcc taatgtttca acagcaggta attttctaat taaagcattt
     7021 cttttagcca tacgctgtac gcctaaagcc attacaatag tagctgttgc aggtaatccc
     7081 tctggaataa ttgatatggc aagggaaatt gcaatcataa acattggcaa gagaggtctc
     7141 tgataaaatg cacctattgc aaatataaca acgcatacaa taatacctat gacacttaat
     7201 gtctttccaa cagtattaag tttacgtttt ataggagtat caaaatcatc ttgtgaatct
     7261 aaaagatgtg ctatgttacc cacttctgta tccataccag ttgctactac aacacctaca
     7321 cctctaccat atgtgactat tgaagatgta tacgccatat tgctacggtc tcctaataca
     7381 cattctttag gtaagattgt atctgcatct ttttctgatg gaacagattc tcctgttaaa
     7441 gaagcctctt gaatttttaa acttgatgtt tctattaaac gaaggtctgc tggaaccata
     7501 gctccatctt ctaaaaaaac aatatctcct actacaagat tacttgctgg aataacattt
     7561 tcttcaccat cacgcataac acgagctgta ggtgaattca tattttttaa tgcttctaat
     7621 gatgattctg cttttttctc ttgaacaatt ccaataactg catttactgt tacaattatt
     7681 agtattacaa aagcttctgt aaattcgcca aatatcaaag ataatattgc tgctcctatt
     7741 agtatcataa ccatagcatc tgtaatttga ctccaaagca tttgtaaagt tgttctaggt
     7801 ggtttttttc taagttcatt taagccattc tctttaagcc gtttttccgc ctcggtatcg
     7861 gtgagcccct cctctgatga atctagactt tctaaaattt catgcgttgc tcttgaatgc
     7921 caaggcatct gtttgtgatt ttccattttg gaagaccact cctttctttt tttaatttaa
     7981 gaataattat attttaaaat caagttcaat tcaatacaca ctaagtatat tttacctaaa
     8041 tttaacaatg ccatttaata tttaacaaaa cttctactct tttacaaata tgtattcact
     8101 attgttagat aagttttctt tatacttata accttctaaa tcaaactttt tcagttcttc
     8161 aatatctctt atcctatttt ttacaatgta ttttgtcatc atacctctag ctttctttgc
     8221 ttgtgtagat tccactttca atatgccatt tttatttact ttaaaagtac atgtaatata
     8281 tgtatcacta tctttaataa atttttctat ggatttgctg tattcatttg atgaaagatt
     8341 aacgatagct gttttttctt tgattagttc tttatatata cgatttcccc aatattcgta
     8401 taaattctta aactcaccta cttgtaattt tgtctgcatt tctaatctat attcatatat
     8461 actatcatat ggttttacaa caccatacaa tccacttatg attcgaatac agctatttgc
     8521 aaactcttca tctttccttg taaaattttc tgcttctata tttttatatt gtattccatc
     8581 ataagttaag attgcagggt ttcctaatct atcaaattta atgctttcaa atctattttt
     8641 atttaataca gctaattttt cattaatttt cattttattt ttaaaatctt ctataccaaa
     8701 tgtctttata ttttccacta tttcttttgt gttatctata aagcaaggca aactcaaatc
     8761 tctatcaaat acttctatat tcttcatatt tttagctggt gataacatta taatcataaa
     8821 ttcttattac tcctgttttt ccaaatattc aagaatctct tgtgcatttg tgtctggttt
     8881 tgctttttca aagacttttt caataattcc attttcatct ataatatatg tcgcacgtac
     8941 aactcccaat gcagtttttc catacatttt tttctctttc catacatcaa aagcttttat
     9001 agcaactaag tcagggtcag aaagtaaaat aaaaggaagt tcatgctttt ctgcaaattt
     9061 ttgatgtgat tttatactat ctttacttat cccaataact tgtatatctt cctttttaaa
     9121 tccatcatat gcatttctaa aagcacaagc ttgacgtgta catcctggtg tattatcttt
     9181 aggataaaaa tatactacta cttttttgcc tttaaaatct gacatactta ccttatttcc
     9241 atctttatct tctagtgtaa attctggtgc ttttgttcct atactcaaca taaatatctc
     9301 tccttttgtt tttattaata attttatact atg
//
LOCUS       DAYASV010000144         9303 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYASV010000144 DAYASV010000000
VERSION     DAYASV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.149"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            163..792
                     /locus_tag="ACGI68_003203"
     CDS             163..792
                     /locus_tag="ACGI68_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139624.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINDFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            793..1575
                     /locus_tag="ACGI68_003204"
     CDS             793..1575
                     /locus_tag="ACGI68_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139625.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1568..2446
                     /locus_tag="ACGI68_003205"
     CDS             1568..2446
                     /locus_tag="ACGI68_003205"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139626.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRISSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2479..3438
                     /locus_tag="ACGI68_003206"
     CDS             2479..3438
                     /locus_tag="ACGI68_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0139627.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3753..4445
                     /locus_tag="ACGI68_003207"
     CDS             3753..4445
                     /locus_tag="ACGI68_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0139628.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4686..5459
                     /locus_tag="ACGI68_003208"
     CDS             4686..5459
                     /locus_tag="ACGI68_003208"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139629.1"
                     /translation="MYLGSSFLGRNISVILKQQHKLYQSMIQEHKKIHSEHLTFINQW
                     IHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLDMAMHFARADNFQKDFIVEK
                     AKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDVKWMKFIIGQLITNGVKYSK
                     DYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFDPFFTGENGRKFGESTGMGLY
                     LVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKDVNSLIKV"
     gene            5727..6578
                     /locus_tag="ACGI68_003209"
     CDS             5727..6578
                     /locus_tag="ACGI68_003209"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0139630.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6682..7428
                     /locus_tag="ACGI68_003210"
     CDS             6682..7428
                     /locus_tag="ACGI68_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139631.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALLRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7428..>9303
                     /locus_tag="ACGI68_003211"
     CDS             7428..>9303
                     /locus_tag="ACGI68_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0139632.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVIMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRTNGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKSISFEDNECISLLSSSLPGNNNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFFIVR
                     N"
BASE COUNT         3614 a          880 c         1650 g         3159 t
ORIGIN      
        1 ttaaaataaa ttacatatac tatttaatat ttcatttaaa atattaaaaa atgtagataa
       61 aaattgtttt attaagaaaa aattaatata ttgacaaaat ttaagtgctc atttaaacta
      121 ttaattgata ataatattca atattaaata ataggaggcg ttatgaataa atatcttcta
      181 ataaatccag tggcagaaag aatgtatgga gaaaagtttc accttattaa agcaaatttg
      241 ataaaaaagg ggtacataat agcagaatgt gaaccacagt tagaatatgt aaaaaagcag
      301 tacaaagagt atacaaaaaa gactgaaaat acaatgttgg attgtagatg tccagaaagt
      361 ataagtcttt taaaaagaaa taatttgata aatgattttg aagtacctat gatagaacca
      421 atattagtaa ggacttgtag ggttttatac gataaatata taaaaagtaa agatgatatg
      481 ttggtagtaa cctgtccatg tacacaatta agagactttg caagtgaaaa gtttaaggat
      541 aagggtaatg ctatgtttta tacatggaaa gagtttgctg aacaagaagg tgtaaaatca
      601 cttggaaaaa tagatgaatc accaattcca ttaggatatt tcaaagatac ttttgaaaaa
      661 gttcttgaag tatcaagtga agacgaaata ttaagtgaaa tacaaaaaat aagaaatggc
      721 tttgaagata agtatgacat agtagaaatg ttttattgta aagatggttg taataatgga
      781 gatggtttgt aaatggtcaa taaatttaat aataagtttt taaggctagt aaagagtgtt
      841 ttgatatttt ttataattct tttattatgg aaaattacaa attatcttgg tatttggagt
      901 gattatatat taccatcacc tgaaaaggta tatagtacat ttttaaatat gataagtgat
      961 ggttcaatat ttattaatgt atatgcaagt atgaaaagag ttttgatagg ttttgcaata
     1021 agtactgcta taggtgtgcc tttagggata ttctttggaa tttatagtgg agtatacgaa
     1081 tactttaaat ctctaataaa ttttttaagg aatacacctc cccttgcttt aataccaatg
     1141 cttatattat ggtttggtat aggagaggaa tcaaaaatta taattatagt tttagcatcc
     1201 tttttcccaa tctttacaag cacattgaaa gggattaaaa attgtgactc aaaactgatt
     1261 gaagtgggta gagtatttga gttttcaaag cttcagataa tatttaaaat aataatacca
     1321 aatgccattc ttgatattgc tgttgggtta aagttagctc ttggatatag ctttagagcc
     1381 attattgggg cagaacttgt agcagcttca tctggtcttg gatacttgat atctgatgga
     1441 aaagaaatgt ctagaacaga tgttgttatt gtaggtataa tcgtaattgg acttttagga
     1501 ataattacag attacatatt ttcgattatt gtgaagaagg taagtaaggg aaagatggtg
     1561 gaagcatatg aataatttag tatgtgactc tggatatata attagaaatt tatataaaag
     1621 ttataaagtt gacaatgaag aacatgtagt tcttagtgat atctctttaa acatagatag
     1681 aaaccatata acagtaattt taggtgaaag tggttgtgga aagactacct tgcttagagc
     1741 aattgcatca cttgaaggta taaatagtgg tgaaattagg tttattaatg ataataaaga
     1801 gtatagacct aatgttggtt ttgtttttca ggagagcaga ttaatgcctt ggttaaacgt
     1861 aagtgaaaat atagcttttc ataatcaaag aaaaaatact attttaaaca aggtgcttag
     1921 aaaatttaaa cacaataaaa gaattagcag taaaattata aatgataatt ttagaagctt
     1981 agaaacaaat gataatttga acaaatcaaa taaatataat aggaaaagta atattgatgt
     2041 agaaaaatat cttaagatga tgaacttgca gaaatttaaa aactcttatc caaatgaatt
     2101 gtcaggaggt atggcgcaga gagtaagcat agctagagct ctttctttca atccagatat
     2161 gttgcttatg gacgagcctt tttcggcatt agattatttt acaaggattg atatgcaaaa
     2221 tgaagttata agaatacata agtcaactaa taaaggcgtg atatttgtaa ctcatgatat
     2281 tgatgaagcg ttaaagatag gcaagaaaat agttgttttc acagacgaaa gaaaagtaaa
     2341 agaatttaat atagaagatg gttataatag agatttaact tcagattact atattaaatt
     2401 aaaaaaagat attttaatga caatgaaatc aaataaaggc atttaaagaa attaataata
     2461 ttttaaggag taaagtaaat gaaattaaaa aagctattga taggtataac aatattaaca
     2521 cttgcaacag catctttagc tggatgtgct aaaaaaaata atggagaaaa actttcagaa
     2581 ataaatttaa cttatgtaaa atcaccatta aatgttcctt ctataattca aaaacaggat
     2641 gatttatttg gaaaggaatt taaaaaggat aatataaaag ttaatttcca cgaaattact
     2701 acaggtccag agcaaacaca agctttagct gctggagaaa tagacttctt acatgcacta
     2761 ggtggtacat cagctttaat agctgcatct aatggagtag agttaaagat attaaacact
     2821 tatagcaggt caccaaaagg atttatgatt ttgactaata ataattcaat aaaatcagca
     2881 gcagatttag taggtaagaa ggtggcaggc ccaaaaggga ctatattaca tcaggtattg
     2941 atttcagcac ttgataaaga aggattaagt atggatgatg tggaatttgt taatatgggt
     3001 attccagagg catcagcagc tctttctgat ggcagcgtag atgctgcatt gattgctggt
     3061 ccagcagctc ttaaagctat gaagtcaggt agtaaacttg tagcaaatgg tgaaggttta
     3121 gtagatggta taatagtaac tgctgttagc acagactttg cagagaaaca tccagaaata
     3181 gttgaaagat ttatgaaggt tgaaaaggaa actttagaat atgtaaataa taattttgat
     3241 gaagctatgg aaaaagtagc taaagaagtt gatttaagtc ttgaagaaac taaagaatta
     3301 tatgcttggt atgattttag tttagatata acagataaag acatatcttc gcttgaagat
     3361 acacaagact tcttaataaa aaataagtta caagagaaaa aagtgaacat aaaagaatta
     3421 atctataatg ctaagtagaa actatgataa aataatacta agtaagaaat atagtaaaag
     3481 tttaagttac cttattaagg taactttttc tattttgaat tagtaatttc aatttatttt
     3541 agaatgatta gttgataata atgattaatt aatcataaaa agatactttt ttatagagtt
     3601 ttattaagat gtctaataat atggttatac ttatgaagtc agtaattgtg aagttagtaa
     3661 tatgattaaa tttatatatt gtaaaaagat tataatatat taaaattgga ctgttaatat
     3721 gatgagtaaa taggtatatt aggaggaatt atatgagtta taaagtatat attgtggaag
     3781 atgatatatc aataagtaca ttacttaaag attatattga gaaatatgga tttgaagtga
     3841 ttgttgaaga aaactttgat gatataatga ttacatttga aagttttaag ccacacttag
     3901 ttctattaga tgtaaattta cctaaatttg atggtttcta ctggtgtaga tgtattagac
     3961 aacagtcgaa tttacctata atttttatat cagctagaga tggtaatgta gaccaaatta
     4021 gagctttaga aagtggagca gatgattata taacaaaacc tttttactat gatgtagtaa
     4081 tggcaaaaat aaagagtcat ataagaaggg tatatggtga atattcacct aaaatagacg
     4141 aaagaatagt ggagctagat ggattaaagt tttatccaga gaggttagaa ctagaattaa
     4201 atggtgagaa tctaattata actaaaaaag aaggtatact gattgaatgt cttatcaaga
     4261 gatacccaaa agtagtaagt agagattatc ttttagaaaa gatatgggat gatattgagt
     4321 ttgtagagga aaatacttta aatgtaaatg ttagcaggat aaggaaaaga tttgaggaat
     4381 taggtataga taatgctgtt cagactgtcc gagggttagg atataaatta aataagaatt
     4441 ggtaaggaga aagtatgaag ttatttttaa aagattataa aggttatatt tttatatatt
     4501 atataggcat agttatgaca attatttact gtaatcttat gaagtttatt agttttggag
     4561 agagtttata tattctttta tttagttctt ttatattgtt atgtttttta atatataagt
     4621 attataaaaa tagagaagta tataatctgt ttgaaaaagg agtacattct ttggaggagt
     4681 catttttgta tttaggaagc tcattcttgg ggagaaatat atcagttatt ttaaaacaac
     4741 aacataaatt gtatcaatca atgatacaag agcataaaaa gatacatagt gaacatttaa
     4801 ctttcatcaa ccaatggatt catcagatga aaacaccaat atcagttata agccttcagc
     4861 ttcaagatta tgagggagaa gaagtaactg aaaatataag aaaagaaata gatagattaa
     4921 ataaagggtt agatatggct atgcattttg caagggcaga taattttcag aaagacttta
     4981 tagttgaaaa agccaaatta aatgaaattg tatctagtat tgtaaatgaa gaaaaaaggt
     5041 tatttataaa aagtggaatg attcctaaga ttcaagtaga caaatttact tatgtttata
     5101 cagatgtaaa atggatgaaa tttataatag gtcaattaat tacaaatgga gtgaaatatt
     5161 ctaaagatta tagtaactac ctaactataa aatctgaaga aaataataaa tatgtaattt
     5221 taagtataat agatgaagga attggaattg tttctaaaga tttaaaaagg gtatttgacc
     5281 cattttttac aggagaaaat ggtcgtaagt ttggagaatc tacaggtatg ggtctttatc
     5341 tagtaaaaaa ggtatgcgat gatttaggtc ataaagtaag tataaaatca gaagtaggaa
     5401 aaggtacaga agtttcaatc tactttaata aagatgtaaa tagtttaata aaggtgtaaa
     5461 tagtttgata aagatgtaaa ttatctgtta caatagggtt tttagttaat tgtacttttt
     5521 tattatacaa tttattaaat gaaatattca tagttaaata atcaaaattt aagagcgttt
     5581 atttgcaaac cttacataga tgtaaggttt gtgtaatgtt tgcagatagt taaattttct
     5641 aatctaaatt acaattaata taagaaataa cataagagaa ataacataaa aaaagaaatt
     5701 ttgtaaaaaa ggaaggatgt ttattgatga gtttagatgt tatttttata ttgaaatcag
     5761 tcataatagc tatagtagag gggcttacag aatttattcc agtttcatct acaggtcata
     5821 tgattttggt tggtaattta atagatttta aggggcaatt tgctgaaatg tttgaggttg
     5881 taatacaact tggggctata ttagcagtag tagtacttta ttggaaaaag ataaaagata
     5941 gcgttataga attttttaaa tttatattta ctggtggaaa agaaggaaag attggtttta
     6001 gatttggaat gaatgtaatt ataggttgta taccatttgc gattatagga gttctatttt
     6061 acgataatat aaaatcattg tttaatttac aatctgtaat tataggattt atagtgggtg
     6121 ggattttatt attagtagta gaaactttgt ttagaaaaaa gaatcattcc acagataata
     6181 tagataaaat tactccaata caagctttaa aggttgggac attacaagtt ttgtctgcat
     6241 ggccaggtat gtcaaggagt gcatctacta ttatgggtgg atggatagca ggacttaatt
     6301 ctcctacagc agctgagttt tctttctttt tagcagtacc agctatggtg gcttcttcag
     6361 gaaaagattt atttgaattt gattattcaa taatgacacc aacattgtgg attgctttgg
     6421 tagttgggtt tatagtagct tttattgtat caataatagt tatggaaaaa ttcgtgaatt
     6481 ttttaaagaa aaaaccaatg agagtgtttg ctgtatacag aataataatg ggtgtagtac
     6541 ttgcagtatt agcttttact aacataatta gtgtatagaa aaaatagatt tatcatctat
     6601 aaaaattaaa ctaagataaa ttataattaa caattatata tatgttattt caaaaattaa
     6661 attaggaagg atagttaaat aatgaaaatt ttaactgtaa ataatttaag taaagtatat
     6721 ggtaaaaaaa taattttcaa cgcattaaat gacataaatt ttagtataga agatggagaa
     6781 tttgtaggaa ttatgggtcc atctggtagt ggaaaaacta ctcttttaaa tatgatttct
     6841 actattgaca agccaactac aggaactatg gaattaaagg gcaagaatcc tttattgtta
     6901 agaggtgagg aacttgcatt acttagaaga agagagttag gttttgtatt tcaggatttt
     6961 aatttattgg atacattaac tataggagaa aatatagtat tgccattgac attagataag
     7021 gtcagtgtaa aagagcaaga tgagagattg aatgaagtat ctacaatatt aggtataaaa
     7081 gatttattag gaaaaagaac ttttgaagta tcaggtggtc aagctcagag gactgctatt
     7141 gctagagcat tgattaacaa tccatcaata cttttagcag atgaaccaac aggtaattta
     7201 gattcaaagt catcaaaggt tgtaatggag ctatttcaaa agataaataa agaaaataaa
     7261 gttactacta tgatggtaac tcatgaccca ttagcagcga gctactgtag tagaatctta
     7321 ttcataaaag atgggtctat atataatgaa atatataagg gtagtagtag agagcagttt
     7381 tatcaagaga taatggatgt acttacatta ttgggaggcg ataactaatg aatataaaac
     7441 agtttgctat taacaatatt tcaagaaata ttaaagcgta tttaggatac ttagcaagta
     7501 ttgttatatc atcttcactg ctattttctt ttattatgtt tacaagtcat ccagatttag
     7561 atgtatctca atttcctgat tatttaaaag aaggtctcaa gatgagtaag ataattgcat
     7621 atctattctt attcttcttt gtgttttatt cagttagtgt gtttcttaaa agtagatata
     7681 aagaatttgg aatactttat attacaggta tatcaaagcg ccaagtaatg aaattaatct
     7741 ttatagaaaa tatgataata aatatagtat cttctgtaat tggaataata ataggcttaa
     7801 tctttgcaaa gatattttta gtgattatgt ctactttttt agaattatca ccattaaaat
     7861 tctatatacc atttaatgcg atattgtcta ctttaatcta ttttatgatt ttagctgtat
     7921 taacatcaat atttacttct tttatagtta aagaaaatca ggtgttgaag ttacttaaag
     7981 gtacaaaaac tccaaaacca gagcctaaaa catcactaat gcttgctata ttaagtattt
     8041 gtttgtttat aatagcgtat tataatgcta ttacctcaac cgaccaatat acaacatatc
     8101 tgcgcttagt tccagttaca tcccttacta tagtagcaac ttacttgttc ttttctcaat
     8161 ttagtgtatt ttttatgaag aagctaaaac taaacaagag attttataga aaaaatacaa
     8221 atatgctttg gatttcgaac ttgatttata aggtaaagga taatgctaga atatttttct
     8281 tgataactat aacttctgca gtagcattta ctgctatagg tacagtttat tccttttgga
     8341 aagatgtaga gcgtcaaatt aatttaatat atcctaacac aatatattat tcaacaatga
     8401 cattgcataa tgaccctaaa aagcctgata gcaaagaaaa agacaaggaa agaatgatgt
     8461 ttatagaaaa taatctaaaa aaagagaatg tagactatac aaggactaat ggtgaattta
     8521 aaactgtatt tcccaagaaa agtgatttta atgtcagaat tatgaaagaa tctaagtact
     8581 tagaaattac agaaactata ggggttaagt ctataagttt tgaagataat gaatgtatat
     8641 cattattatc atcaagttta ccaggaaata ataacgtcaa agaaaatatt attgtaggga
     8701 ataggggttt aaaggtggca aaacaagtgg aagaatgtgt tatgccagca tattacaaaa
     8761 atatgtatgt ggtaaaggat aatttttatg atagtataga atcaaaattt atagtggata
     8821 gatttactgc atttgaagta gaggattcaa gtaaaactat agagatatgc agagaatttg
     8881 aaaataaatt taataatgag tcagggatgc aaccatattt gtttttctct aaagaattaa
     8941 tgataggaac tgggaaatta atgtattcta catttatatt tttagcaata tttataggtt
     9001 taattttctt tgttactaca agtagttttt tatataataa attttatatg gattgccaag
     9061 ttgataagaa aaagtatgaa cagttaaata aattgggtat gacatataat gagattaaaa
     9121 aagcttcaac aattgagatt ggaatagtat ttttgtttcc ttatgtagtg gctgttatac
     9181 attcaatatt tgcacttact gcacttaaaa attcttttga tatagaagta gcatcatcag
     9241 ctgttttggt aatgggaagc ttgttcttgg ttcaaatagt ttattttttt attgtaagaa
     9301 ata
//
LOCUS       DAYASV010000145         9150 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYASV010000145 DAYASV010000000
VERSION     DAYASV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.150"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            505..1833
                     /locus_tag="ACGI68_003212"
     CDS             505..1833
                     /locus_tag="ACGI68_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0139633.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIIIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
     gene            2586..3419
                     /locus_tag="ACGI68_003213"
     CDS             2586..3419
                     /locus_tag="ACGI68_003213"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0139634.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDQMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            3488..4444
                     /locus_tag="ACGI68_003214"
     CDS             3488..4444
                     /locus_tag="ACGI68_003214"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0139635.1"
                     /translation="MNKKLYIGMVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVL
                     SATNNYINGQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVD
                     SKYQELEDKMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDM
                     EAYYTSHKKDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESF
                     ESLAKKYSDDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVK
                     VTDKRERQKSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            4496..4978
                     /locus_tag="ACGI68_003215"
     CDS             4496..4978
                     /locus_tag="ACGI68_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0139636.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            4990..5859
                     /locus_tag="ACGI68_003216"
     CDS             4990..5859
                     /locus_tag="ACGI68_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0139637.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            5902..6657
                     /locus_tag="ACGI68_003217"
     CDS             5902..6657
                     /locus_tag="ACGI68_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HFH0139638.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLASLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KEDNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            6699..7520
                     /locus_tag="ACGI68_003218"
     CDS             6699..7520
                     /locus_tag="ACGI68_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HFH0139639.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            7525..8286
                     /locus_tag="ACGI68_003219"
     CDS             7525..8286
                     /locus_tag="ACGI68_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFH0139640.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    8447..8704
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8817..9056
                     /locus_tag="ACGI68_003220"
     CDS             8817..9056
                     /locus_tag="ACGI68_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFH0139641.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         3865 a          874 c         1528 g         2883 t
ORIGIN      
        1 aaagctaata tttcattttt aaataaaaaa ataaaatatt agttttgatt tctggtttaa
       61 attgggttat aataaattgt caaattattt attaatataa aatagactat aaaacaagca
      121 aaaatcatac tattatataa aagaccttaa agatagaaaa ctacagaaag aagccaaaat
      181 gtttttgaaa aaaatgaaat tttattgcaa aaaaatcaaa atgaatttaa cttaatagtt
      241 ataatctatt tttagtgtta caaatataca atgtatatta tacaatataa aaaatacatt
      301 tacttagaag tattattata tttggatttt tggtttttat aattttttta tatttaaaca
      361 taactaaaaa taaaagatta ctaaagctaa gtagagttta ttttatttta ataaataata
      421 aagctatttt tctatatatt ggcataatat atgcttattt aactatgaaa ttaaaattaa
      481 ataggattaa tgaggaggaa aaaaatggga agtaatgaac tgaaaaagac tcttggcgta
      541 tcagcagcat tatcaactgt tgttggttcc gttataggag cgggcgtatt ttttaaacca
      601 caagcagtct atacattaac tggaggagct ccaggtcttg gtatattggc atggttaata
      661 gctggaatca taactataac agctggtctt acagctgcag aggtatcagt tgcgatacca
      721 aaaactggtg gaatgatggt atatataaaa gaaatttatg gagaaaaatt aggtttttta
      781 actggatgga tgcaaattgt attattttat ccagggatga tggcagcatt aggtgttata
      841 tttggagaac aagcatcggc actaattggt agtccatcac ttttacttcc aatagcaatt
      901 ggaattataa taatagttgc agggcttaat atgttaggtt caaaaactgg aggagtcata
      961 caaactgttt ctacaatttg taagttaata ccacttattt taataatggt tgttggattt
     1021 attaaaggtg gtggaaataa tccaatactt acacctatgg ttggtgaagg attaagcctg
     1081 ggtagtgtac taggtcaagt tttaattgct atattatttg cttttgatgg atggatgaat
     1141 gttggtactt tagctggaga aatgaagaat cctggaaaag accttccaaa agctataata
     1201 ggtggattgt cagttgttat ggctgtttat tttataataa atttagcgta tctttgggta
     1261 ttaccagcta atgaattagc aaattatgct tctcctgcat cagcagttgc tgaagttata
     1321 tttggttcaa tgggtggaaa aataatatct gtaggtatat taatttcagt gtttggagca
     1381 ttaaatggat ttttactaac tggttcaagg gtggcatata ctttagctac agataagact
     1441 ttaccaaagt atggtatatt ttctaaatta aattctgctc aagttccagc aaatgcaata
     1501 gcattagtat ctgttattgc atctatatat gctttgtcag gtcaatttaa tttattaact
     1561 gacttagctg tatttgctac ttggatattc tatgtattaa cttttatagg ggttatgaaa
     1621 cttagaaaga ctcatccaaa tataccaaga gagtataaag ttccattata tcctattgtt
     1681 cctataatag ctatagcaag tggtatattt gttgtagtta atcaattgtg ttttgcaggt
     1741 atgaaaacta ctatgatatc tataggaggt cttgtaataa cagctatagg cttaccagtg
     1801 tatgcttata tgactagagg agtaaaaaga taaaccaagt agtttacaaa tgcttcataa
     1861 ttaaaataaa agagactgtc tcaaatagag ataagtctct tttttattgt taaaatggta
     1921 aagatatgtt taattttaca aaaaaagagc gttccatgaa ctaaaaagtt tattttaagg
     1981 tattcatttt atttttagtt ttttgattat aaaattaaac atctaaaaaa ttatttattt
     2041 taaaatactt tattttaaag tgttttttat caaactaagt ggtatctgta caacaaatat
     2101 tatacatagc ttttatgaaa attaaatctt tctgttatat ataatagaat attgtattat
     2161 atagaaaata ttacctgatt aatatagtga tttataaatg agattagaaa attttatttg
     2221 ataacaaaaa ttagataata tgaatatata atgtatgtat taaaaatgtg gcaaatagtt
     2281 gttttgctat ttattatttg tttaatattt ctaatttata aaaaaaaaat tataaatttt
     2341 aaaaattata aatgacattt taactagaat taattgtaaa aaacactata taatactcaa
     2401 ttttcaacat tctatacaat tatacagaaa ttatacagca taataatcac cgaaataaca
     2461 aattttaaaa attaagcaaa actttttgtc gaagttcaac gtctatatat atgtagtgta
     2521 aatttttggg ataaaatact acaaaaatat aaatgtaaaa atatagtaat attggaggtc
     2581 gaacgatgag aaataaaaag aaaaaaataa atagaaagaa gttgtatttg cttttaagtg
     2641 ctattgtagt gtgtctggct tttatagtag ttggtgttcg cgtcagctct ttaaatatga
     2701 aggaaaatga aattagtaga aatgcaaaac tgtcaagttc tactattaca gatatagtat
     2761 ctaatacatc aatagaagtt agtaaagggc caagtaaaag ttctggaaaa atagcataca
     2821 taactataga tgatggtcca tctaaattta ctgaccagat gataaaaact ttaaataaat
     2881 acaatgttaa agcaactttt tttatgatag atgggaatat gaaggaatat ccacagcaag
     2941 ttaaaaatat aattaaaaat gggaacacag caggttttca tagtgtatca catgatattc
     3001 acaagcttta tgttactagt acatcagcaa aagaggaatt tgatacaaat gatcaaactt
     3061 tttataagat aactggtaaa cattcaaaag taataagaat accttatgga agcaaaccat
     3121 atacaccaca agcctcttat caggctttag ttgatgctgg atataaaatt tgggactggg
     3181 acttagatac agaagactgg agatctaatt cttctcaaat tgtacaaaat gtaaaaaatc
     3241 atattaagaa tagaaaaggt gaagataaag accaacttgt agttttaatg catgagaaaa
     3301 aacagagtgc agaagcttta gattcagttt taaaattctt atcagatgaa ggttatgaat
     3361 ttgcgccagt agatcaaaac caaataccaa aaaattattg gttacgtaat ttagagtagt
     3421 taaaatataa gaatattgat tgatattttt agaaattgag aataaaaatg agtgaggtgt
     3481 aggtatattg aataaaaaac tttatatagg aatggtcgga atattgtctt tgatgatggt
     3541 tggttgtaat aaaagtttag caaaagtaaa tgatgtggaa atcacaaaag aacagtacaa
     3601 aaaaacaaaa gcagttttat ctgctactaa taattatata aatgggcagt ctttagatga
     3661 attagaaaaa actttggata aaaaaggtag aaataaatta gaaaatgtta taatatcgtt
     3721 tatggtggat aatgaacttt tatatcaaga ggctaaggat aaaggactaa ctccaagtaa
     3781 aagtgaagta gactcaaaat atcaagaact agaagataag atgaatttga atacaagcta
     3841 taaggaaaaa atggacaaag caggtgttga taaagattat ttaaaacaag aaatatctag
     3901 agatttagca atagataaaa ataaaaaggc ctttgaagat agaataaaca taagtgataa
     3961 tgatatggaa gcctactata caagtcataa aaaagatttt aatgtagaag aagtaagtgc
     4021 ttctcagata cttatatcga ctttagataa aaataaaaaa gaagtaagca aagataaaaa
     4081 agaagctttg aagaagaagg cagataatat attgactaaa atcaaaaatg gagaaagttt
     4141 tgaaagtttg gctaaaaaat attcggatga taaagcaact ggaaaaaatg gaggacagct
     4201 aggatatttt acaaaggatg ataaaaatgc tgagtttaca aaggaagtgt tcaaattaaa
     4261 gaaaaatgaa gtttcaaatg tgtttgaaac tagttatgga tatcatatag tcaaagttac
     4321 tgataaaaga gaaagacaaa aaagttttaa tgagtgtcag agtttaataa gagaatctat
     4381 tttaaatgaa aagtatattg agcatataaa aaaactgaat gaagatgcta aaatagacag
     4441 atagaatttc taacagctca atcctagcct gtaatcggag ggagatttta caggaatgtt
     4501 taagagttta aagagaacga aagttgagaa atacattatc gataataaag actcttttta
     4561 tagaatagca tatagttata cgaagaatga agaagatgcc ttagatgtgg ttcaagaagc
     4621 catgtataag gcactatact cagtggaaaa tattaaagaa gtaaattata taaagacatg
     4681 gttttataaa attttagtta gaacgagtat agattttatt agaaaaaacc gtaaatataa
     4741 taatatgaca gatattgacc ttatagatga gactggagag tatgataaat atactgattt
     4801 ggatttaaga agagctttgg aagaattacc tattgaatac aaatctatta taatattaag
     4861 attttttgaa gatttaaaga ttgaagaagt agcaataata ttggatgaaa atgtgaatac
     4921 tgttaaaact aggttatata ctgctttaaa aaaactaaaa ttaaaaattg aagagtaggg
     4981 ataaaaagaa tgacaagcaa agaaaggtta aataagttaa aagatgagta tcataaaatt
     5041 ccaataccca agaaattaga tactataata aatcatgaaa aaatagaaaa catatatcga
     5101 gagaaaaata aaaaagtaaa tagattaaga tttaaagttg caatagcctt tgcatgtata
     5161 tttacagtat tagtaaatat aagtcctgta tttgcagaca atttttctaa gatacctgta
     5221 attggagcaa tagtagaagt aataactata aaaaattata gtttaaaaag tgaaaattat
     5281 gaagctgaga ttgatattcc taaaatacga ggtttaaaag ataaaaactt agaacaaaga
     5341 ttaaacagta gttttatgga agatggaaaa aggctttatc atcaattcca agagagaatg
     5401 gagaagatac aatcatcaaa gaataaagga tataaatctt taagtttgag ttatagtgta
     5461 aaaaataatt ctaaaaaatt tctatcaata gagatgacaa aaaatgagat agaggcttca
     5521 tcatatgtga gtaaggtaca ttatactatt gataaaaaaa gacaaatagt tttgactttg
     5581 ccaatgcttt ttaaagatga taagtatata aaagttataa gtgataatat aaaagaacaa
     5641 atgagagaac aaatgaaaaa ggatagtaca aaatcctatt ttatagatca aaaaaaagac
     5701 ttgcctgttg aagattttaa aacaattaat aagtatcagg atttttattt taataaaaat
     5761 gaagaccttg taatttgttt tgatgaatat gaagtagcac ctggttatat gggcgcagtt
     5821 gagtttgtaa taccatataa agtaataaaa gatttgtaaa ttgaatagta gtttatatat
     5881 aaatctttta aggaggacaa tatgactaca tataaaaaac ttaaaaaatc tatatttaaa
     5941 attgctatat tttcaatatg cttattagct tcattagtag atacaaaccc tatatttgca
     6001 ggtagttcct ttaatgaaac ttcaaatagt gatacgataa aggtgttaaa tgctaagagt
     6061 atcaacttta aacaagacaa ttatgaagtg aatgtgagta ttcctaaaat aaaaggttta
     6121 aaagataaaa ctttagagaa taaattaaac aatgagtttt tgcaagatgg gaaaaaaatt
     6181 tacagtgaat ttcaaaataa aatgaaaaag gaagataata agggtcatag atacctaaat
     6241 gtaatccata atgtcaaaaa taattctaag gactttttat caatagaaat gacaaaagaa
     6301 gagatagaag cttcctcatc tataactaag aagcattaca ctgttgataa aaatagagga
     6361 atactattaa atttaccaat attttttaaa gatagtagat atattagagc tataagtgat
     6421 aatataaaag agcagatgag aaatcaaatt aagaaagatg atacaaaatc ttattttata
     6481 gaccaagata aagactcgcc agtgaaagat tttgaatcta taaataagta tcaagatttt
     6541 tattttaata aaaatggaaa tcttgtgatt agttttgatg aatatgaagt agctcctggt
     6601 tatatgggag cagtagaatt tgtgatacca aataaagtaa taaaaagact aagatagata
     6661 tatagaagat ttttagatag actggtaagg agaattatat gaatatatat gaacaagcaa
     6721 taagattaat agaaaataat gaggatttcg catttgctac aataacgtct cattcaggtt
     6781 caacaccacg tgaaactgga gcaatgatga ttgtgaaaaa tgattctact atatttggct
     6841 cagtaggagg aggtagtgta gaagcagaat gtataaaaca tgcaattaat gttataaaaa
     6901 acagagaatc aatgttgtat aaatttactc taaacaaatc tgatgttgca aaactaggaa
     6961 tgatatgtgg aggtactgga gaaatacaaa tagattttat agatagtaaa ttaaaaagta
     7021 atatagaaaa atttaataag agactaaagg aaaatacaag taaagcatat atatttggtg
     7081 caggtcatat ttcaagagat gttgctgtga tactatctct tttagaattt agaacagtag
     7141 taatagatga tagagaagaa tttgctaatc atgaaaggtt tccagaatca gaagtagtag
     7201 ttttagattc ttttgaaaac ataccagatt ttccaactga cgaaaatagt tatataatta
     7261 ttttaacaag aggtcatcta tatgattcaa gtgctttaga atgggctctt aaaagagagg
     7321 ctggttatat aggtatgatt ggaagtagaa caaaaattgg attgacttac gaaaaactca
     7381 tgaaaaaagg ttttaagaag gaagaattat caaaagtaca tgcaccaata ggcataaaat
     7441 tgaatgctca aacaccagct gaaatagctg tatgtatagc tgctgaactt ataaattgta
     7501 gagctaataa ggaaaaataa actaatgaac acagggagta aagatattga tactttaaag
     7561 gttaatttta gagggattga aataaaagca gttttagtat ataaaaatag aaaaaatatt
     7621 tctataaaaa tagatccatt tggcaaaata actgtaatga gtcctcctaa aatatccaaa
     7681 aaaataataa aagatataat tattgaaaaa ggagactgga tacttaaaaa gtcagaaaaa
     7741 tgtaaaggaa gagaggaagt atataaacaa agaatgttta taactggcga aaaatttctt
     7801 tatttaggtg aagagtacca tttagttata aaagaactgt tgagagattc tgtcaaaaaa
     7861 agtaactgta gaataactat taacgaatat caaatagttg ttcagacaaa tgatacaagt
     7921 acagatttta ttaaaaaatc tttaaaatct tggtataaga tggaaagtga aagaatagta
     7981 ttagagagaa tagattttct taaattaaat tgtgaaataa tgaagcaatt agttccagcg
     8041 agtgttaagg taaaagaaca aaataagaga tggggaagtt gtacagctca aaaaaatatt
     8101 tatataaact caaaaatatc tatggcaaga ttagatgtaa ttgattatgt aataatacat
     8161 gagtttagcc atttagtaca tatgaatcac tctaaaaaat tctatgattt agtaaaatcc
     8221 attatgcccg attataaaga taaagaaaat tggctaaaaa taaatggata taaaataata
     8281 atctgaaaac gattattcca aaatataggc gtaaagtata aaagaagtat tattgtgaaa
     8341 aaaataaata atctgaaaat tttaaaaaaa gtcattgaca ttgtaaatga tagggaatat
     8401 aatctatatt aacaacaaaa cagaattaaa atataaaatt aaaaaattaa aagcaatgac
     8461 agagacaaca atactaatag tgttatccac agagaaccaa tgttgctgag aattggtgtt
     8521 aacagtagta taatgatcac tctggagctt ttgtactgaa tatttttagt aagtacaaac
     8581 gtatgtcagc gttacatgac taaggttaag gtaattaatt acccgacctg aaaaaagagg
     8641 ggtagatgta ttttttatat ctacaaataa ggtggtaccg cggaatataa ctttcgtcct
     8701 tataagtaat atttatattg cttataaaga tgaaagtttt tttattttaa gcgatataaa
     8761 taaatttaat ccatataaaa ttaataaaaa aataataaaa ataaggaggg atttttatgg
     8821 aaaatactgt atatgctaaa ttaaaccaag gaatagattc ttttttaaga gtggctatga
     8881 ctctaagaag aagagaggtt gatatacaat caataagtat gactgtagat aatatgaata
     8941 attcaggtat aaaattaata gtaaatgaag aaaagacatc cttagattct gtcttaaacc
     9001 atatgaaaaa actacatgat ataagagaga taactgttga aagaatgaga cattaattaa
     9061 atatatttta taaagctaaa gataaatatg cttattttaa aataaaaaac taaagataaa
     9121 tattaaataa gaaaaatctt agattaaaat
//
LOCUS       DAYASV010000146         8906 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYASV010000146 DAYASV010000000
VERSION     DAYASV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.151"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(70..678)
                     /locus_tag="ACGI68_003221"
     CDS             complement(70..678)
                     /locus_tag="ACGI68_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFH0139642.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(787..3525)
                     /locus_tag="ACGI68_003222"
     CDS             complement(787..3525)
                     /locus_tag="ACGI68_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139643.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINICISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYTKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLKDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3673..4464)
                     /locus_tag="ACGI68_003223"
     CDS             complement(3673..4464)
                     /locus_tag="ACGI68_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0139644.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4500..5156)
                     /locus_tag="ACGI68_003224"
     CDS             complement(4500..5156)
                     /locus_tag="ACGI68_003224"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFH0139645.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5149..6114)
                     /locus_tag="ACGI68_003225"
     CDS             complement(5149..6114)
                     /locus_tag="ACGI68_003225"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFH0139646.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6220..6326)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6616..7560)
                     /locus_tag="ACGI68_003226"
     CDS             complement(6616..7560)
                     /locus_tag="ACGI68_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139647.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSESIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLIKSKVLELYNHHCI"
     gene            7671..8138
                     /locus_tag="ACGI68_003227"
     CDS             7671..8138
                     /locus_tag="ACGI68_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HFH0139648.1"
                     /translation="MENDTIKADDILNYCLSNLDDVVLMDSWGERAIYYNPNGVLKRG
                     VYVLTIKEKDSNNDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8308..>8906)
                     /locus_tag="ACGI68_003228"
     CDS             complement(8308..>8906)
                     /locus_tag="ACGI68_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139649.1"
                     /translation="KLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNNE
                     GYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRDK
                     IQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQND
                     FIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2886 a         1480 c          872 g         3668 t
ORIGIN      
        1 ctataaaaaa ataagataca tacctataaa ataccttcct atagatacat atcttaccta
       61 tataaaaact tattctatat aaaaacgcca taaaaaatct tttgcttctt ctgcataatc
      121 tatatttata cgtttactag tttttatatc taactcatct ttactaaata ctttttttcc
      181 tttatcatca taataaaaat cacttatata taattcttca cccattatac tcttactatt
      241 tagacttcta tctattttta aagctttgca aagttttcct ggtccgttag taatattttt
      301 tacttgatat ttactcaact cagtgtaagc tttgttgtat ctattcatag atatctcatc
      361 taaagaagtt ataggctcta cccctcttat taatacacac tctggcacat tttctatatt
      421 tgcagataaa ttcaagcaat tatacatacc ataaattaaa taaacatata tatgtccacc
      481 atctaaatat aatggcttag ttctatctgt ctttttattg ccatatacat gtgctccttt
      541 atcatttaca cccatgtatg cttctgtctc tatgattttg gtaacaatta ctttattttc
      601 atatttcctt attagatatt tacctaatat tgatttggct aaatcaattc cattttttct
      661 aaaaaaatcc ttttccaaaa tttcacctca taaaatttat ttaaaacgca tcaaatacaa
      721 ctaaagtaat aaataaaatt aactcttatt aatgtgatat atataaacaa taaatttaga
      781 catttattat ttatatatat caaaaaattc tatctcaaac ttttcaatat tatcattgta
      841 atttgaatta tcatatactt cttcaacaac ctctttaatt gggaatataa cttttacatt
      901 agttccttct cctaccttac tatctatgta aatcttccca ttttgaattt caatcaatga
      961 ttttgtaagg cataaaccta taccacttcc aaattcattt gatataccac tgttttcaat
     1021 ttgtttaaat cgattaaata tatcctttaa tttttctttg tctatgccca ctccagtatc
     1081 ttttataatt atctctactg tttcgtcttt tttatatata cctacaagga tttctcctcc
     1141 ttctaaagta aattttatag catttgataa tatatttagt attattcttt ctatcgattc
     1201 tgggtcaagc cctaccataa cagtttcttc atctgtatca aaaattaaat ctattccttt
     1261 agacttagta taactactaa tagatgtaac tattctttct gttatgtcaa taatatcaaa
     1321 attaactgct ttaatcttat atatgtctga cttaacttga gaaatattta ttatattatc
     1381 tattaatctc aataatctaa acatattttg tttagttatt tttatatgtt cttttagtat
     1441 atcctgaaaa tcaattattt ttcctttttt ataaaggttt tcaataagtt gaactgaact
     1501 atatatatta tttaaaggtg tcttaagttc atgagatata ttagcataaa actccatctt
     1561 catttggtca tactgtcttt gttttattaa tgctttttta agtctttcag actctctttg
     1621 cacacttaaa tctttaataa ttctcactct acttcttttc tgtccaaagt ataatgttct
     1681 tgatatcatt tttacttctt taatgtgatt attttcattt actatatgga tactcttctc
     1741 tctttctcct atatttaaat tgtttatgta tttttttact aatatatttc tatatttttg
     1801 tgttgtaact cctaatacat cactagaaat tccgaacaac tctctaaagg ctctatttgt
     1861 atatagtact cttgatgtac ttaaatcctc aagaaaaata gcctctggta taacatccag
     1921 taatttttca tatattattt tttgttcatt tactgttcta agaatattta tctctttagt
     1981 tatatctcta aatgtaagca atttaaagag tttattatct gaccaatagt ctccaactct
     2041 aacttcttca acatgttctt caccttctga gtcagacatc acaaaattaa ctatacagtt
     2101 tgctgtgtca ttgtaactta ctatttcagc tttcttttct tgtgttagat ttaatctttt
     2161 aaattcccta ttgtcaaaga aatctatgtt aaaagtttca aaatattgtt ctaatgtcat
     2221 atcgactaaa tcacttgaaa cacatttaaa actcttttca tattttttat ttatacttat
     2281 taccttattc ttctttatgc caccttcagc acttcttaca agtataggag atggaatatt
     2341 tagtataatg ttttcatagt ctttatttat atttcttatt tcttcatcta ttttgatttc
     2401 atttgtaata tcatttaaac ttattaagta attgctttcg tctttattat tacttaaact
     2461 taacaaataa attttatcat ttaactctac cagtttatca attatactta tatcgttatc
     2521 aaatttcttc aatttttcag caactttttc atcttccttt ataaaagtta atattttata
     2581 ataattattc atatcctttg aaaattcatc tttaaattta ttatttgtgt aacttatatt
     2641 aaaatcatag tctgttacta tccatacatt tggtattgta tccagagctt gtctaagtaa
     2701 ttcttttctc tggtctatgt agttttttaa tgttctttgt ataatcatat ccttttctat
     2761 tgctaaattt atctttccta attgctctga ctttttactt aattttttat tgattgaatc
     2821 aagttcataa tatggataca ctactctatt attaagactg tatacatata cataataagt
     2881 atggttacat aatagtaaat atattactcc tattaatcta tctgaatcta ctatattaat
     2941 cttagaaatt gctacaaaac atataatagt tgtaaaaaaa acaaaatatg ttaaatcttt
     3001 tattaactta tagtgctcaa tgttactatc aagtttattc aacaaatata gtaaattaaa
     3061 catagatata caaatattaa ttaatataat aatcatattc cagttaatat cactaaaaat
     3121 atttcttata aagtaatcta atatcacaaa agcactataa aaaattagta ttgggcttat
     3181 tcttagtttt tttactttag aaatggttaa gtatataaaa gtagacatat taattatgct
     3241 taagttaagc aatacaattt ggaatttttg aatttttaaa tttactactc ctaaaatact
     3301 tacaaaaact aatacaagac accctacttt caatatattt ataaattcat ctgttttaaa
     3361 gctacttata taaactatag aatatccaaa tatccaaagt aaaattattc tatacatatt
     3421 tatagaaact gtatacaaat ttatatttaa aagtgcaata accaaaaata acccattagc
     3481 tagtatatag accttattct tacttaaagt tcctttagat tccatattcc ccaccacttc
     3541 ttcattttta tatagtttta cctataaata tgttaccaca attcttccaa aaaatttgtc
     3601 actttctagt tataaaaaaa aatgagtaaa tatttttaat tttactcatt ttgtagattt
     3661 tgtatattca gtttaaaaag ctggaactat acttccttta tattcatctt gtataaattt
     3721 tttaacttct ttactattca tagcctctga taaagcctta atcttatcac tatctttatt
     3781 attttctcta caagctatta tatttacata tggagaatca cttgattcta ttactattgc
     3841 atccttagta ggatttaagt tagctgttaa agcataatta gaatttatta ctgctccgtc
     3901 aacatctttt aatactgttg gtaattgctc tgcattcatt tcttttatta ctatattttt
     3961 aggattttta gttatatctt ttttagttat aagctctcca tcttttactt ctatcaattt
     4021 attttttgct aataatttaa gtgctctagc tccattagtt gcatcatttg gcacagctat
     4081 aacagctcca tcttttaatt catctaatga cttaactttt tctgaataga atcccattgg
     4141 ctctatatga actttagatg tataagtaag tttatatcct ttctccttca cagtttcctc
     4201 taaaaatgga atatgttgaa agaagttagc atccaaagac ccttcatcta aagctgtatt
     4261 tggaagtaca taatcatcaa atatttttac ttctaaatca tatccttttt cttttaacaa
     4321 aggttttgca acttccaata tctttgcatg tggatttgat gaagctccta ctagtatttt
     4381 tttatcctct ttgtttgaac aacctactgc tgatattgct attgctgaca ccaatgctac
     4441 tgataataat ttctttaatt tcatttttac tccccctctt taaaatgttt ataattaatt
     4501 tattttagtt tcttataagc cagatttcct actccctgga ttatctgaac taatattata
     4561 agtgctatta cagtatatac cattactgct gtatcaaatc tttgatatcc ataaataagt
     4621 gctatattac ctaatcctcc agctcctact gcacctgcca tagctgtata accaagtata
     4681 gatattattg ataatgtaat tccagatact attgaaggca tagcttcctt tatcataact
     4741 ttaaatacta tttgtctttt tgtagctcca aaagattttg ctgcttctat caatccttta
     4801 tcaacttcat ttaatgagct ttctataatt ctagcgataa acggtgctgc cccaatagtt
     4861 attggcacta ttgcagcagt ttcccctata cttgttccaa cgattaatct agttattgga
     4921 atcattgcaa ctataagtat cataaatggg aaacttctaa atatatttac tataaatcct
     4981 aatgcactat ttattgtgct atttggtttt aatccatctg gttttgtaac tactaatatg
     5041 attgctaata taaatcctaa tattgttgcc actatagttg gtactataac catatacaaa
     5101 gtttgtaaca atgcatttgg aaatagtgtt gttaaaaagt caattaaact attcatttat
     5161 catttcctcc catcttatgc ccttactagt tagatattgt tttacttgct ctccactttt
     5221 atctgaaatg tttataataa gtgaacctaa aatatcatct ttaaattgtt ctaatttccc
     5281 aaatattata gacacatcta tatttagttc tcttgccata gtcgtaataa tagcttcatt
     5341 agaaatatct ttagggaata gtatctttat atttgttcct tttggcaata ttatactttc
     5401 ttcacctatt aattttctaa gtcctttagt atttcttaag aatatttctt ctgtatctcc
     5461 tacctctaat acttctccat tttccattat tgctactctt ccacaaatct gctttataac
     5521 ttccatttga tgagttacaa ctattattgt tattcccaat tttttattta tatcttcaag
     5581 caatgaaaga attgactttg tagtatttgg gtctaatgct gaagtcgctt catcacaaag
     5641 aagtacttgt ggatttagtg ctaaagctct agcaattgca accctttgtt tttgtccgcc
     5701 acttagattt ctgggtttat catttttctt ttcagatatt ccaactactt ctaataactc
     5761 taaaactctt tttttaattt ctgcctttga gtatccaaaa cattctaatg gcaatgctac
     5821 attatcaaat acagtttttc tttcaagcaa tgaaaagtgt tgaaaaatca ttcctaactc
     5881 ttttcttaaa tctctcattt gcttttcatt caatgacttt acttctttat cagaaacaag
     5941 tacactgcct tcttggtatt cttctaatcc gtttatacat ctcagtagtg ttgatttccc
     6001 tgcaccacta tgacctatta ttccaaaaat ttctccagat tctatttcaa tacttacatc
     6061 tttaagcact tgaatctttc cataatactt attaacattt tttatgctaa tcataaaatt
     6121 tcctcccata tattctgctc agtttttatg tttatcctct cttatgtatt acaacaaaat
     6181 aaaagcccga gtataaactc aggcttagaa agctatttat actcatctat caactatatt
     6241 agttgcagga tttagcacca atgtatataa aatatactgg ttgccgggtt tcaaagggcc
     6301 agtccctcca ccactcttga taagacgtta tttaatttaa ttctattatc atttgttgct
     6361 atgttatata tgatatattc attaatagta tttgtcaata aatttattta aattttttat
     6421 tttttattat cgttaattaa ctatttttaa ttagttttct agctcctaac actttatatt
     6481 ttaatgtcta tataactata gaacatcatt ataagaaata aattaaataa catcaatcta
     6541 tctaaatcat ctgtaaagta aattaagaaa attaacattt tatccaaact ctatcctact
     6601 attaaataaa ataatttaaa tacaatgatg attatatagc tctagtactt tagactttat
     6661 caaatgttta agttctatag gttctattat tgtagcattt ttcccaaaag ttaaaaaata
     6721 ttcagccacc cagttaagtg tagattttgg tatatacata tctatgtatc cacctttaga
     6781 caagattttc aaccctcttg ccaacaaaaa ttctgtttca caacgtttta ctccctcatc
     6841 tgtcaaatta attttaatat gataatctgt acctacttcc atattcttaa gataatcatg
     6901 tatagaagat aaaaccttat atttcttact tgataagttt tctatactca aaatcttcac
     6961 aattctgtct acacgaaatt ccttaataga ttctgatttt atacagtaag ctggacaata
     7021 ccaaagtcca ttcatggcat acaaaccaat aggaacaatg gtacgtacac tatttttttg
     7081 ctttgatgaa tattcaattt taattgtaaa ttcattcatt actatattaa acaattcttt
     7141 caataatggt gttgaacagt gcctatctgg tgtccaaaaa acaaatttac gccttatatt
     7201 ctcaatatta tattgaatat catttggtat acaatttaaa aattttttta aaacagaaat
     7261 agtttcttgc tcaaatggca aatcattata atattctagt gactgatatg caaagaatat
     7321 agattttacc tcactttctg aaaatgcaat cggtggaagt atacgctctt taagtacatg
     7381 atatccacca gccgctccaa cttttgaata taatggaaat ccaatactct ccagcacctg
     7441 taaatctctt agtatagtac gttttgatac agaaaattct tctgctaatt ctcctgctgt
     7501 aaaacttctt tttgcattta ttgttatcat cagttcaatt aaacgctcag ctcttgacat
     7561 atttatactc ctaatttaaa tatagtgaca ataattgtca ctattatatg ataagatatt
     7621 cttgcatcta aagcaataat aatagtaaag aaagaaggga ttgatacttt atggaaaatg
     7681 atactattaa ggctgatgat attctcaatt attgtctatc aaacttagat gatgttgtac
     7741 taatggatag ttggggggaa cgagcaattt actacaatcc taatggtgtt ttaaagcgag
     7801 gggtatatgt tcttaccatt aaggaaaaag acagtaataa tgataaaggt tcgttagtta
     7861 gtcgtccaaa tgtataccgt gtgaatatag gattaaaaaa agaaactttt attgaaatgt
     7921 ttggatatat tccaaagcgt ccaggtgtag gtcaaatagt tgatatggat tttgatttta
     7981 caaaattgga cacaatcatg ccacatccta tctactcatg gatgggatgg atatgtgtcc
     8041 taagccctac tgaaaagaca tttgagaact ttaaaacttt aataggagaa tcttacaatt
     8101 ttgcaaaaca aaaatttaaa aagagaaaaa acaagtaaaa acgtgtgaaa ataacctcta
     8161 aataaattat aataaaaata ttaaggttac aagataaaaa ttaatatctt aataacatgc
     8221 aaaaaagcga agccaaaaaa tgtattattt ataaggcttc gctatatctc aaataattta
     8281 tttcaaattt caaaactatt tattctatta tttcttatta tttcctgtta ttcctaatct
     8341 atcattcata tcttttatca tttttctaaa accactacct atatcatttc caacatcttc
     8401 agttccatga actaatttta taaagtcatt ttgacctttt ttcatatctt catcagatac
     8461 cttatctaat ttttctattc tcttatctat atcttttact aatctttccc cttcagcgat
     8521 taggtcatca ttcattttct ttaactctgt atcttctgtt ttaccatctt ttttaagaga
     8581 tgttacaaat gattgtattt tatctctact atctttatat gcagccttta catcacttag
     8641 atatttttca tttcctggat attctttatc tttaaactga ttatttatac tttctggagt
     8701 atcataaata gaatatctat ttagacctgc tatataatca ttatagcttg ttgaataata
     8761 attcatatat ccttcattat tatttacaga ttcattagga ttttttcctg tattttcact
     8821 tggtttttca ttatttttag tacatgcagt tatacctata gctaatacta gtgctaatcc
     8881 tacaaataaa gttcttttta atttca
//
LOCUS       DAYASV010000147         8906 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYASV010000147 DAYASV010000000
VERSION     DAYASV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.152"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            161..502
                     /locus_tag="ACGI68_003229"
     CDS             161..502
                     /locus_tag="ACGI68_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFH0139650.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            653..1066
                     /locus_tag="ACGI68_003230"
     CDS             653..1066
                     /locus_tag="ACGI68_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HFH0139651.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDDIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1268..1909
                     /locus_tag="ACGI68_003231"
     CDS             1268..1909
                     /locus_tag="ACGI68_003231"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFH0139652.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1933..3186
                     /locus_tag="ACGI68_003232"
     CDS             1933..3186
                     /locus_tag="ACGI68_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0139653.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3232..3882
                     /gene="cmk"
                     /locus_tag="ACGI68_003233"
     CDS             3232..3882
                     /gene="cmk"
                     /locus_tag="ACGI68_003233"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFH0139654.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3933..4529
                     /locus_tag="ACGI68_003234"
     CDS             3933..4529
                     /locus_tag="ACGI68_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFH0139655.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4542..5381
                     /locus_tag="ACGI68_003235"
     CDS             4542..5381
                     /locus_tag="ACGI68_003235"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFH0139656.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5626..6780
                     /locus_tag="ACGI68_003236"
     CDS             5626..6780
                     /locus_tag="ACGI68_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HFH0139657.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7086..8783
                     /gene="ade"
                     /locus_tag="ACGI68_003237"
     CDS             7086..8783
                     /gene="ade"
                     /locus_tag="ACGI68_003237"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFH0139658.1"
                     /translation="MGNLKRQCDVSSGREKADIVLKNGTIINVFTEELITGDVAIVGD
                     TIVGIGDYKGNVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNN
                     PRILGLGEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLD
                     KMEVDSVYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFT
                     AEEVQEITDNMLEIARKMEVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT         3657 a          851 c         1575 g         2823 t
ORIGIN      
        1 tttaacttta aaaattaatg ttaaaaaata aacttataca ttaatattaa taataattat
       61 ttaaatatct taagtaaatc agtcttacat atatttccga tagattatat tgataaaaaa
      121 cactaaaatt aaattaataa aagtacttgg agggatggaa atgaaagagg aaaattttat
      181 taatgttgaa tacgaggata aacgtttttc aattaatagt aaagaagttt tgtacataga
      241 aaagcaagag tatgaagtaa agatttatac tgaaagtggt aattatgaag ttggagaaaa
      301 tgctccttgt ataagaaaac tattgaaaga agaaaatttt ttcaaatgca gtaatagtta
      361 tattgtaaat ctagacaagt gtgaatttta tctcagcaat aattgtattt ttgtgaataa
      421 taattttata agaattttag gttcagaaag aaaaaagaaa ttaaaaaaga aattagaaaa
      481 attatatgat tatgaagatt aatattggga ggctgtattt tatacagcct ctttatttat
      541 tgattgtatt attaataaat tttaaattat gataaaatat aaaacaagta acttatttca
      601 gaataaaata ctttttaaaa gaaagggacg agatatggga gattataata aaatgcttga
      661 attaaagaat aacttaggta tcagtgaaag aaagttaaaa tatccttgta tctataaaca
      721 tttcaaaggg aaatattatg ctacaatggg tcttagcaag gctattgatg atatagaaaa
      781 catatgtgaa atatatggta aagaaaattt aatccaaaac agaaataaat ataaattagt
      841 tattagacat actgaaagag aagaagatat ctatgtttat agggatttag atggaaactt
      901 ttatcataaa aaagaagaag atactaatga tttagtttta tacaagacct tatatgatga
      961 tacaggaata tttgcaagac ctttggatat gtttttagaa aaagtagata cagataagta
     1021 tgtcaattca attcaagaat acagatttga agaagtatat aaataatata agagaaatat
     1081 gtttataaat ttagaaaaat tctttaacag tacaataatt tgcagaaata caaatgagat
     1141 ttattgtaaa taatatgtta gattagatta taaaatagaa acaaagtaaa gtattatatg
     1201 atataattaa gttttgagaa gtgataattt atatttaata aaaaataggc atatattagg
     1261 aggacatatg ggaaatactt tttatatagt aagacatgga caaacggatt ggaatatatt
     1321 gggaaaaaca caaggtcatg gtaattctga ccttacgcct caaggtatag aacaggcaaa
     1381 agaactttct gaagatattg gcaagtattc aattgattat atttttagta gcgaccttgg
     1441 gagagcaatg caaacagctc aaattttagg agataagctc aatattgaag ttcaaaaaac
     1501 tgaggcactt agagaaatgg gattcggagt ttgggaaggc cttttaataa aagaaattca
     1561 aaaagattat tcagatatat atgcaacatg gagaaatgaa ccacacttag taaatatacc
     1621 agaaggcgaa actcttaaga ttataaagga aagagtagat gcttttataa aggaactaaa
     1681 tgaaaaatat gataataaaa atataattct tgttacacat tcaattacat tgagggttat
     1741 gttgttgtca tttttagaat caggtatgga aaatatatat agaattaagc aagataatac
     1801 tgctttaaat atagtagaat ttaaagatta tgggcctgtt atagtaaaaa tgaatgatac
     1861 aagtcatata aaaaatcatg taaagataaa taattctgca ctggagtagg aatcaattat
     1921 aaggagaaat ttatgagtaa agttatagta gttggtggag gagcttctgg aatgatggca
     1981 gctttgtcag cttcaaaaaa caataatgaa gtaatattag ttgagagaaa tggagaatta
     2041 ggaagaaaac ttagggcaac tggtggcggt agatgcaatt ttaccaataa tagagagata
     2101 gaagattttt ttgataaagt tgtcagtaat aaaaaatttc tctatagtag tttttataca
     2161 tttacaaata aagaccttat ttcgtatttt gaaagtagaa atttggaata taagattgaa
     2221 gaagaaaatg accacaaagt atacactaaa aatgataaat ctatagaagt aatagaagta
     2281 ttaaataaag atttactcaa tcacaatgta aagataatgt ataataagaa agttatagat
     2341 attataactg aagaaattgc tctaaaagat gattctaata aggacaagtc aaagtatcta
     2401 ataaaaggga ttattttaga taatggagat aaaatactag gagacaaggt tattatatca
     2461 actggaggag tgagttattc aaagacgggt tcagatggtt caatgtataa gattcttaaa
     2521 aagcatggac atacattaaa caaactatat cctgctttag ttccacttac aatagaggaa
     2581 aaatggatta aagatttaca aggaatatcc atgaagaatg tggaaatatc ttgtaagatt
     2641 aagaaaagga aaatatcaaa atctggagac atgctatttg cccattttgg gataacagga
     2701 ccttgtgttc ttataatgtc ttcttatatt aacaagatta ttgaaaagga aaaggtagaa
     2761 ttaaatatag atttcttacc aaatttaagt actgatgaaa tctctagtat aatcagagca
     2821 tttccaaata aaaatgttct aaataatctt aagcaaatac tacctcaaaa tttcttaaaa
     2881 gagatttttt ctctattatc tttagttgat aaaaaggcaa gtgatttgtc taaggcagat
     2941 gaaattagaa ttattgagta tataaaaaat atgaagctaa cttgtaatgg gactacaggt
     3001 ataaacactg gtatggttac ttctggtggt atttcagtta aagaaattaa ttcttctact
     3061 atggaatcta aattagttaa gaacttattt tttacaggtg aagtaattga tatagatgca
     3121 gaaactggtg gatataattt acaaatagca ttttcaactg gatatcttgc gggtataagt
     3181 gtataaatat aattttttaa taattaaata tgaataaata gaggtgtata tatgggtaat
     3241 ttagttatag ctgtagatgg tccagcagga gctggaaaaa gtacaatagc caagatagta
     3301 gcaaaaaagc taaatataaa ttatatagat acaggtgcta tgtacagagc agttacttat
     3361 aagtgtttaa aaagtggtat tgatgtcaat aatgaaaaag aagttattca aatagctgaa
     3421 aattcggata tagattttaa agataacaat atatatttgg acaaagaagt tataaatgaa
     3481 gaaataagaa caatagaagt aagcaataat gtttctaatg tagcaaaaat aaaagaagta
     3541 agacagttga tggttgaagt tcaacgaaaa ataggaatga aaaattcagt aatactagat
     3601 ggtagagata taggttcata tgttttccca gatgcagatt acaaattctt tttagtagct
     3661 actccagaag aaagaggaaa tagacgctat aaagaacttt gtaataaagg atataatact
     3721 actttagaag aagtaattga ggatataata agacgagatg aaattgattc aaatagagaa
     3781 tttgcaccat tggtaaaagc taatgatgca ctagaaatag atactacagg aaaaactata
     3841 gaagaagttg tagaagaagt tgtatctaaa ataaatcttt agttttttat gcatcataat
     3901 gaaaataaat gtagtgttga agggagataa acgtgaattt ttatagattt gtaataaata
     3961 tttttaaggg tttttctaaa atatttttta aatatgaagt tataggtgca gagaatattc
     4021 cagatagagg aaatatagta atagcatcaa atcataaatc gaatcttgac cctatattct
     4081 tggctgctgc catagaaaat agagaaatag ctgcaattgc aaagaaagaa ttgtttaaag
     4141 taaaaccatt gggatttata ttaaaaaaat tgcatgttat gcctataaat agagaaaaac
     4201 cagatgtatc tacaataaaa actattttaa ggtcagttag agacggttat gtattaggaa
     4261 tatttccaga gggtactaga ataaaaggag attcttttgg taaggctaag gcaggtcttt
     4321 ctgtatttac aataaaaagt aaatctaaag ttgttccagt atcaataata tctaaatata
     4381 aactttttag taaagtaatt gtatatattg gagaaccaat atcatttgaa gaacatttta
     4441 aggaaaaatt gagtaatgat gaccatgaaa ggatatcaca agagatttta gaagtgataa
     4501 aacaaaatta ctttaaatat tcaaaataag ggggtaaagt tatgaacata aaaatagcaa
     4561 aaaatgctgg cttttgtttt ggagtaaaaa gagcaatgaa aatggcatgg gatgaagttg
     4621 aaaaaaatga ttctggcata tatgctttag gtcctcttat tcataacaag caagcagtag
     4681 caaaatatga agaaaaaggt cttaagactg tgaatgaaat tgatacaata ccaaatcatg
     4741 aaaatatgat aataaggtct catggagttc ctgaaaatat atataaggaa gccaaagata
     4801 aaaaattaaa aattgtggat actacatgtc catttgtaaa gaaaatacat acagtagtaa
     4861 gtgaatatca taataaaggc tatgaaataa tagttattgg agatatgaag catccagaag
     4921 ttataggtat aaatggatgg tgtgaaaatt cagctataat aataaagacc ttagaacaaa
     4981 tggaaaatat ggaatttgat aactccaaaa gatattgtct tgttgcacag actacaataa
     5041 atccagagtt gtatataagt atagttaata aactatcaga taaattagaa gaaatagtat
     5101 ttaatgatac aatttgttca gctacaaaaa caagacaaga atcagctaaa gagttagcta
     5161 aagaagttga ttgtatgata gttataggag gtaaacacag ttcaaatact caaaagttag
     5221 taaaggtttg tgaagactta gtaccaactt ttgcaataga gactaaagat gaattggatg
     5281 taaatacgct taaaaaatat aaaaatttgg gtattacagc aggggcatct acacctaatt
     5341 ggataataga agaagttgta acattccttg aaaatttata atttgatagg taataaaaat
     5401 aggtggacat tgttataatt tatcataata tccacctttg atttcatatt acatgtggta
     5461 aatagataac ctaatatcaa aaagtgtgta tcttaagatg aaaaactaag agtataagat
     5521 gcgcactttt taatattaga aattcaaaag aaatattgat attgattatc aatagaaaat
     5581 agaatataat ataattgttg caaaattagt ctaggaggta aattaatgga aatgttgata
     5641 tccgcttctg aagaggcgtt tttacatgta ggttctatga ttggattttt tattttgtta
     5701 tttggatata ttaattataa aactagtgga aattttacta atatcatatc aaaaaacaga
     5761 aaatttcaac ccttaatagg tgctttaatt ggagctatac caggttgtgg cggaagtttg
     5821 gcaataatgc cattgtatat aaatggaaaa ttaagctttg gagcaataat tgcaagttta
     5881 atagctagta tgggagatgc agcatttgta cttatttcat caaatattaa aatgtatttt
     5941 tttgtcacta tagttagtac aattacagga ataataactg gacaattagt ggattacttt
     6001 aaactagaag aaaaattagg gttaaaaaac cgtagaaaat cagataaata tgatataagt
     6061 aaaaataacc ctaaagaaaa taaagaacaa cataatcatg atgaaataat tttagacact
     6121 ctagctaaat ctcatggaaa tacaaataga cttgcattta taataacaca tggaaaggga
     6181 tataaaattt atataggaat tatagtaatc ggttttatat ttatgtcact tgctcactct
     6241 ggacttaatc ttcctataat tgagaagtta cattcattgg aagaagtaat agcagtgatt
     6301 ggaatattat tttcaataat ttatatgtgg tgttttaaaa aggtatttaa aaattctaat
     6361 caaaaagagg aagaaaataa aaagatttct ttgcgagaaa tgcttatcca ttctgttgga
     6421 gaaatatcat ttgtaattac atggattttt attgcatatc ttatttacga tttgataata
     6481 ttaattttag gtggagatga atacttagtt aaattagtat tatcaacagg tgtaataagt
     6541 gtatttatag gtgcaggtct tggacttatt ccaggttgtg ggatacaaat tgtgcttatg
     6601 tcattttatt tgaagggaac tataccactt ggtgcagtga tagccaactc tatttctcaa
     6661 gatggagatg cacttttccc acttttagca atggataaaa aatcttcttt atgggctatg
     6721 attattacta ctataccagc aatacttgta ggtatgatag tttatgcatt tttaggttaa
     6781 atataattat gaataaagaa gtattaaaaa aataataaag cacataaatt tatttataag
     6841 aatagtgaag attaaatata aaaataaaaa attcaatatg aattaaatta ttgcttaaat
     6901 attaatgaga acatgggaga taatattatt cctcatgttc tctctattta attaaaggaa
     6961 ttatggaaca aatttcaagt gatactttct atcaaaactc atatataata tatattaata
     7021 aattaaacta taatacgtaa tacagtcagt atattgatat aaaaaactta ggaggagttg
     7081 taaaaatggg taatttaaaa agacagtgtg atgtttcaag tggaagagaa aaagctgaca
     7141 ttgtactaaa aaatgggaca atcataaatg tatttacaga agagttaata acaggagatg
     7201 tagcaatagt aggagacact attgttggaa ttggcgatta caagggcaat gtagaaattg
     7261 attgtagtaa caaatatata tcacctggct ttattgatgc acatatgcat attgaatcta
     7321 caatggtaat gccaattgaa ttatcgaaaa aattattaaa atcaggtacg acaactatca
     7381 tagccgaccc acatgagttg gtcaatgtaa aaggagttag tgcaatagat tttttattag
     7441 aatcgacaaa agatatacca cttaatgtat atattatggt gccttcatcc gtacctgcaa
     7501 caagttttga aactaatgga gttggtaaat ttagtgcaaa agacatggag agttatgtaa
     7561 ataatcctag aattttaggt ttaggagagg taatgtgttt taatgatgtt ataaattctg
     7621 aaaatgaaat cttggataaa ctagagcttt ttaaaaataa agttgtagat ggacatgcac
     7681 ctaatataaa tggtaaatca cttcagacat atgtatgtgc aggaatagaa aatgaccatg
     7741 aatgtataac ttttgatgaa gtatatgaaa aattgagagc aggattaaaa attttaataa
     7801 gagaaggaag cgcagcaaaa aatttaaagt ctatagtaag tggtatgcta aagcataact
     7861 tgccaattga agaatttatg ttttgcacag atgataagca cttagatgat atagaaaaac
     7921 aaggtcacat aagatggaat ataaaatgtg cgattgattt aggtatggaa cctgttaggg
     7981 ctataaaagt tgcaacttat aattctgcaa aagcatatgg gttaaggaaa attggagcaa
     8041 taggtgcggg ttataaggca gatattgttg tattgaatga tttagacaaa atggaagtgg
     8101 atagtgtata taaagatggt aatttggtaa acgaagagat gttttctaat tacaattatg
     8161 agattaaaga taaagaattg cttaatacag tcaagtttaa atatattaat aaagaaaaaa
     8221 tacagttaaa agtaagtgaa aaaaattatg taatggagat tgtaccttat caaattttga
     8281 caaataaggt atatgaatct ttaccatgtg cagatggata ttttgttcca aataaagagt
     8341 attcaaaact ttgtgttgtt gagagacata gaatgactgg aaatgtagca attgctccac
     8401 taaaaggatt tggcataaaa aatggagcaa ttgctactac tgtggcccat gattctcata
     8461 atattatagt tgcaggggat aatgatgatg atattctagt agctataaat tacttaaagg
     8521 aaatacaagg tggttatgta attgtatcaa atggaaaagt tcttgcacac ctaagtcttc
     8581 aagtagctgg attaattagt acttttacag ctgaagaagt acaagaaatt actgataata
     8641 tgttggagat agcaagaaaa atggaagttc cagagtatgt agaccctttt attacacttt
     8701 cttttatggc acttccagtt gttccacaaa taagacttac agatttaggt ctatttgatg
     8761 ttgaggaatt taaatttata tagatagtat aaaagtaata ataaatgagg ctttctcaaa
     8821 atagagataa gtctcttttt attgttgaaa tgatgaaaat atatttgatt tcatacaaaa
     8881 aagagcatct catgaactaa aaattt
//
LOCUS       DAYASV010000148         8892 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYASV010000148 DAYASV010000000
VERSION     DAYASV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.153"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(310..873)
                     /locus_tag="ACGI68_003238"
     CDS             complement(310..873)
                     /locus_tag="ACGI68_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139659.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(1032..1745)
                     /locus_tag="ACGI68_003239"
     CDS             complement(1032..1745)
                     /locus_tag="ACGI68_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HFH0139660.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            2037..3701
                     /locus_tag="ACGI68_003240"
     CDS             2037..3701
                     /locus_tag="ACGI68_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFH0139661.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLKQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(3808..4707)
                     /locus_tag="ACGI68_003241"
     CDS             complement(3808..4707)
                     /locus_tag="ACGI68_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HFH0139662.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(4734..5372)
                     /locus_tag="ACGI68_003242"
     CDS             complement(4734..5372)
                     /locus_tag="ACGI68_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HFH0139663.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(5709..6707)
                     /locus_tag="ACGI68_003243"
     CDS             complement(5709..6707)
                     /locus_tag="ACGI68_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HFH0139664.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(6805..7380)
                     /locus_tag="ACGI68_003244"
     CDS             complement(6805..7380)
                     /locus_tag="ACGI68_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139665.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            7696..7959
                     /locus_tag="ACGI68_003245"
     CDS             7696..7959
                     /locus_tag="ACGI68_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139666.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(8396..8773)
                     /locus_tag="ACGI68_003246"
     CDS             complement(8396..8773)
                     /locus_tag="ACGI68_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139667.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         3123 a         1357 c          934 g         3478 t
ORIGIN      
        1 aagatatctt tatatattta tagttctgct tattcaacta tatttaacat tatacgtgta
       61 tgtataaata ctttttatac actgtttgtg ctatccatcc cgcacctaaa ggagtgggat
      121 ttttgcacta ttctgtatta tatatatcca aaaatgttat aaaaatacaa atattagcag
      181 aaatttaaaa actgaagaaa gtattctaac taagatttac aaattcttaa tcctatcgaa
      241 gttaaactac tatcttcagc ctaattatct agcaatattt ttttatatga aaaactctaa
      301 atcaagtttt taaatgtttg cgatttttga tacataagaa tgttgtctag aattatattc
      361 tgataataaa ccttcatact ctaaggttaa gccagataaa ttataaaaat catttggcgt
      421 tacttcaaat gcttgtttat ttaaaatgtc tatctcactt ttttctaaca tgtcagcaac
      481 aaaatttgaa catacatatt tatattcaga ttgtctctga atattcatta gtaaatatat
      541 taatgccatt gtatcgtaat agtaatcttc tctataatca ctaattaatt ttatattttt
      601 atatagattt tcatattgta aattatctac ttctaacgaa tataccctac acacagtgtt
      661 ttttcttata gcatataatc cttgatttat attttcttca acaaatcctc caataaatgg
      721 attccttgga tttagccttc caaaagaata cattggtttt agattttcat ttaatgatat
      781 tgacacatgt gtatacagct ttttacttat attccttatt aagtaagata atatagttcc
      841 tgtatatgtt gttacaatat agactttatt catcatttcc ccccaatcta gtaactaaat
      901 aatattatac acttacaatt taatatcata agatattcta acataataaa tgtggtttta
      961 caaattttga ttttaaggta ctttaatatt atataatata ataaaaatat aaatataagt
     1021 tttctatatt tttaaccaac agatgttttc ttcaaattaa tcagatgatt tagtatatct
     1081 tgaacctttc cagagtcctt taaatatcct aatgccccat ttatttgtga ttttaaatct
     1141 atttctactt ccatatccaa atcaacattt ccttttttta tagatatatt tttaagtgtt
     1201 attgggtaaa gataactttt ttccaaagta atcttatttc cattaacctc aaatggaact
     1261 tttttattgt agttacttaa tattttttct agcattttat cacttagttt aaattttcca
     1321 attttcacat tctcaagact tacaactaag tttccatcct ttaaagtagg tctcaattca
     1381 agttcatatt gactatttac caaaccaaat accttatatg gtcctgctac ctttatttta
     1441 ccatttttca tttcaacaaa attattttca agttcattaa ttccatgctt ctgcatcaat
     1501 gtatatatta aatttttaaa ctcttcctct gaaacagata tttttccaac cattctaagt
     1561 ggatttttta ctacctccat agaatttata tatgagcctt ttgataaata ttcttcatct
     1621 atgattttat tattttgtga actaacatca tatctttctt ttggtgtcaa gatatataca
     1681 acaattcctg caagtactaa aacaataaca attaaactta acaaaatctt tgcaaccttt
     1741 cccataatcg tctcctttac aaatctgtca tcagatttaa aataaattta gataactaac
     1801 ttcacataat attcttaaca atatacatac tataatacag tcttttaaaa attaattcaa
     1861 ccaatctaat gttttgtaaa taaactgtaa aaacatgttt ttagtataaa agttaatatt
     1921 ttgcataata ctcttaatta cacctttatt tttttctaat cttggattaa aattaatatt
     1981 atgcaataca gtgtagtgtt ctgtattata atcaaattaa ataggaggta tagaatatgt
     2041 caaatgaaac gaactcatct aactttataa agaatattat tataaacgac ttagaaacag
     2101 gaaaacatga cagtatcata actcgttttc cacctgagcc aaacggatat ttacatattg
     2161 gtcatgcaaa aagtatatgt cttaattttg gattagctaa ggagtttaat ggaaaagcta
     2221 atttaagatt tgatgataca aacccattaa aagaggatgt tgaatatgta gagtccataa
     2281 aagaagatgt aaagtggtta ggatttgatt ggaacgagtt aaattttgca tctaactact
     2341 ttgatgaaat gtataagaga gctttaatac taataaaaaa gggtaaagct tatgtatgtg
     2401 atttgacaca agaggaaatg agagaatatc gtggtactct aactgaacca ggaaaagaaa
     2461 gtcctcatag aaacagaact attgaagaaa accttgattt atttgaaaga atgaaaaatg
     2521 gtgaatttaa agatggtgaa aaaacattaa gagccaaaat agatatgtct tctccaaata
     2581 taaaccttag agacccaatt atatatagaa tatctcattc aacacaccat aatacaggag
     2641 ataaatggtg catatatcct atgtatgctt ttgctcatcc aattgaagat gcaattgaag
     2701 gaataactca ctctatatgt actttagagt ttgaagacca aagacctctt tatgattggt
     2761 ttgttaaaga atgtgagatg gaaaatatac ctagacaaat agaatttgct agacttaata
     2821 taaataatac tgtcatgagt aaaaggaaat taaaacaact tgtagatgag ggtattgttg
     2881 atggttggga tgaccctcgt gttcctacaa tatccggaat cagacgtaag ggatatactg
     2941 cagaagcatt gcgcaatttc tgtagtgaaa taggggtatc aaaagttaac tctacggtag
     3001 atagtcaaat gcttgattat ttcctaagag aaaacttaca accaaaggct cctcttacta
     3061 tgggagtttt aagaccttta aaacttatta taactaatta cccagaagat aagattgaga
     3121 tgcttgaaat agagaataat gctaaagatg aatctcaagg aaagagatta gttcctttct
     3181 ctagagaatt gtatatagaa caagatgatt ttatggaaga acctgttaag aaatatttta
     3241 gatttttccc aggaaatgaa gtccgtttaa aaggtgctta ttttgtaaaa tgtactgatg
     3301 taattaagga tgaaaatgga aatgtagttg aaatacatgg tacttatgac cctgaaacta
     3361 agagtggctc tggctttaca ggacgtaagg taaaatctac aatacattgg gttgatgcta
     3421 aatcagctat accatgtgaa tttagattat ttgaaccatt aattcttgat gatatacctg
     3481 aaaatgaagg taaacatttc ttaaaacaaa taaatcctaa ttcattggaa atattacaag
     3541 gttttgttga acctacacag ataaaagatg ctaaaccttt tgataaattc caatttgtta
     3601 gaaatggatt ctttagtata gataataaat atacaactga tgaaaaattt gtatttaata
     3661 gaatagttcc tctaaaaagt tcttttaaac caggtaaata atcttacaat caaaaaatga
     3721 agacttaaaa tataaattac ttctactata aataaactta gttagaagta attttttatt
     3781 tgagtcttca tcatttactt taaatgatta acttttttca ctacagattc ccatgacttc
     3841 tattccatct tttatgcatt ctatgttaag aggaaaatct ggtccttttt ctccgtcaat
     3901 atctgttata agtgaatctt cacattctat atgcaccttt ttagttttaa agtatagtat
     3961 ttcatttcca ttatattgat ctaaataatc acctctaagc acacttatca aaactggtat
     4021 tgattttggt attggcattc ctttaaaaat aattacatcc aaatatccat catctatttc
     4081 agccttatat gctagattta tatttcctgc tgttttgcca ttaaaaatca acataagata
     4141 catatctcca tcataataca tttcctcaga agatactttt atcttaaatt tcctcatata
     4201 caatgcttct tctattccct ttatataata agaaattctt ccaaatgaat tttttaagtc
     4261 tgtgtttatc ctttgtgata catccgtaaa cataccagca cttgcaacat ttataaagta
     4321 cttattattg actttcccta tgtctatttt ctttggtttt gaatttatga tttcatttat
     4381 agcttctttt atatcaaatg aaatttgaag agcatttgaa aaatcatttg cagttcctgt
     4441 aggcaaaatt ccaattggaa tttttatgtc taattctttc attgcattta ctactaaatc
     4501 tacagtacca tcaccaccag ctatcaatat gtgttcataa gaactatcaa cttccttaaa
     4561 tgcattcttt acaggttctt taatagttaa tctatagggt atcaataaat aatttttttc
     4621 ttgatataat tctatgatag tgtctaaatt tgataatatc tttttttcac ctgaattagg
     4681 attgtatata agttttactt ttttcattat ttctccttac tcattttatt cttttagtat
     4741 ccaaaatctt catttaaaaa aatcacatgt attctttctg gtgttaattg tctttttata
     4801 agtgtataac tacagcatat tcttcctgga cttgaacagt ctatacatat accagtagtc
     4861 ttacatccag tatttgtact taatctttta caattggctg gagcagctat tgtttttgtt
     4921 ctttttatag cttcatctac atctttaact attttattta ctcctgctat aactattact
     4981 ttatcaggtc catatatcat agcagcaact ctattcccat tcccatctaa attgtaaagt
     5041 tctccatcct ctgttatcgc attagtactt gtaaaataag catctgcaga aaatgacttt
     5101 ctatatattt ctttcatttc ttctggtttg atatcttttg catatctatc aagaaaatta
     5161 tatttttctc ttataaaatc aatcactcct gtttcaaata aggtcataga acctccacat
     5221 cctactagag aacctgcttt aactatttct tcaatttttt taagaagctc ttctttgtta
     5281 cttactaaat accctttcat attattgttt tccaaagatt ttattgtttt ttctattctt
     5341 ttttcattaa gccaatttaa attttgatcc attttaactt cccccttatt tttaatatgc
     5401 acttaattat ttataaactt tattttcttt ataagtttat tttagtacat ctaaaaaaaa
     5461 atacaatata aaacttaaat agttttacat aattttttcc aaaataactt actcaatcaa
     5521 aaaaggaaag tagatgtact attctacttt ccctatattg tatgtagtat ttattgccta
     5581 aaatctatat attaattata tgtttcatat tatataatat cacagttaat taaaatgtaa
     5641 tactatcttc tttttaaaca tataaattgt attattaaat ttaatatatc caacttcaat
     5701 aaatcttatt aatttgatga agaagatgct atagctgcac ttgttgctgc cattacacat
     5761 gcaatttgaa tagcaacaac aatatttaaa aatatattat tagttataga gcctatatta
     5821 ttctcatttc gtatcaaatc tgcataaatt gaagctacaa tagcagaact aatcattact
     5881 ctattacttt ttcctaaaga ccaatttcca aagccttttt cttctttaag tttatttgaa
     5941 acatttttta tctcttctat tatttcatct tctctataat ctaataatga aattgctcct
     6001 attattggat atccataata attcatttta caatcatttt ctttaaatgc ttttttaagt
     6061 tcaatagtct ttttacattt aagcatagtt ctatcatcac taaataaaag aatttgggat
     6121 aatgcttgta aatcatttcc tttataaaaa ccattttcat ttagaaattc ataacatttt
     6181 tctatgtatt ctaaatcttc atctaaattt tttgaatgaa ttgcaataag tgctatatta
     6241 caatagtcat cactagaagt tagaaatgga tgttttttcc tcatgtaatc atatattatt
     6301 ttcatttttg atatacatgt aagataatcc attttctctc tattttcata aactattgat
     6361 gctgtaagtg gtaaatatgt atcgccccaa aacttttcac ttttaagttc tttgtatata
     6421 tctaaaatca tcttaaaact actttctgga tttgattcaa acgaaagtag tgttgccata
     6481 taaaacatat tatatcctct aaaattagag aataatccag tatttttttt aataagctct
     6541 attcgttctt ccactaatct aggacttatt ctcttgttct taagggtaaa tgcaagtgca
     6601 caactatgtt gtatcaatcc catttgccaa gaacctttaa cttcatttaa tgctttgtaa
     6661 ttttctatta ataagtttac ttttccattt ataaacgatt gttccatatt gccctcctag
     6721 taatatatct cattgtacta atagttttat atttattata tatatattaa atactacaat
     6781 caatacatat tacttcaaag aacatcattc ttcaacaata atgtagcctt ttttgtaatg
     6841 tcttcccata ttattgactc tatcatagta ctctgattca ctttcattac tccatttggg
     6901 cacaaataca tctattgcat tagaattact aaaatactta ctagaccccc tatccataac
     6961 agtttttaat tttccatcta caataatctt agttccaaaa cttaagtgat tgtttgctac
     7021 aaaaccatct tctaaatcat ctcctgtagc agttatcgct ccatatccat tttcttgatt
     7081 aagacttgtg taataactaa cttcaaaagt tataggtact aacttcttta ttttaatacc
     7141 taactgattt tgtagtttct ccttttgtac tttcaactta tactcatcac tatttttata
     7201 atcttccata ccttgattat acttggaatt aaagctttta cctgacttta ctatagtatc
     7261 tatatttact atagattctt tacaatgatc tgcatctatt tttgtttctc catttaccaa
     7321 taatggagtt aaacatacac tacatacaac acatgtaatc aatgcttctt taagcttcat
     7381 aaattccccc cttattattt tgatatatta gcatatttaa tattatatgt ctacatttag
     7441 atttaaatac cacacccatt tgctttaaga agttgtctta tatttaattc accttaaaca
     7501 cttataaatc ctctatctta gaatcatata gaataaagct tttatcttta aatatttttt
     7561 attaatcttt taaaatctaa aactacttta tctaattttc tcttttttag aatttaagaa
     7621 ttaaaattct ataattttaa aacatattga atttattttt ttgagatgat ataattttct
     7681 taagaggtgg ttgatatgat aaaaaataca aaacaaatta cagtagaagt taatgaaatt
     7741 ttattacaag aaatgtctaa gataactaat gacgaaaata atattttgac agatttgata
     7801 aatgaatcaa ttgtaaatta tattcttgaa aacaaaaaac ataatattga aaataaagat
     7861 ttagaagaag tgattaaatt ttacaaaaaa gaaaatactg aaaaacctaa aagtgcagca
     7921 agagctatag ctgaagcaaa aactcatact ctgtattaat caaataaaaa agttcctaaa
     7981 aactaggaac ttttttatat taataattat taatatatct caatatttat ctcaaatcta
     8041 tcaatataag catgtttaat acaatctatt gtatacttat cttaccctag aaataatcaa
     8101 aacaaaaata aaattatgcc ctttatatct aaaacaatta atttacatga ttaatataca
     8161 aaaattctga cttttaaatt cgtatacaat atcaaactaa aataatatta atgacaaacc
     8221 caatataaat taaattcaaa caggtttgat tttacatcaa acttattgta ttaatattaa
     8281 ataacaagac aaaaatattt ataaaaatca caaattttac ataaaacttt atatttgatt
     8341 catttaacta attattgtaa aaactattta ttttatgata attaactaca ccaaattatt
     8401 catctttttt aatatcctta acccattttt caacagagtt caattcttct tcatttaccc
     8461 cctttttgaa caaggatttc ataaaactct caatagaact tccatgcata ttatctaaaa
     8521 aagtttttgt ttcaaaatgt aaatactctt cttctccaat aagaactgta taatgtgtat
     8581 gtctatcaat ttttcttgca cttaagaatt gcttatttac taatcttgat aaaagtgtta
     8641 gtgttgtagt ttgcttccaa ttatatagtt tttccatagc ttcaatgaca tcttttgatg
     8701 ctactgtaaa atctgaactc caaataaatt tcatgacttt taattctgct ggtggtatct
     8761 tagtaatagt catatgattt tacactcctc tcaaacttct acataattaa atactatatt
     8821 aatagacttt tcgagtcaaa ggttttattt aaaatatctc atccaaattt attaatacaa
     8881 aaaatttata tt
//
LOCUS       DAYASV010000149         8856 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYASV010000149 DAYASV010000000
VERSION     DAYASV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.154"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..484
                     /locus_tag="ACGI68_003247"
     CDS             <1..484
                     /locus_tag="ACGI68_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0139668.1"
                     /translation="LAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLS
                     TKLIKENDIICIEDLQVKNMIRNRKLSRSISDVSWSEFIRQLEYKANWHGRQIVKVGK
                     FFASSQICNKCGYKNEEVKDLNIREWIYPSCNETHDRDINASINILKEGLRLITIQNK
                     "
     gene            916..1170
                     /locus_tag="ACGI68_003248"
     CDS             916..1170
                     /locus_tag="ACGI68_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139669.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            1206..1529
                     /locus_tag="ACGI68_003249"
     CDS             1206..1529
                     /locus_tag="ACGI68_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFH0139670.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIL
                     IDYII"
     gene            1803..1955
                     /locus_tag="ACGI68_003250"
     CDS             1803..1955
                     /locus_tag="ACGI68_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139671.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            2224..3072
                     /locus_tag="ACGI68_003251"
     CDS             2224..3072
                     /locus_tag="ACGI68_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFH0139672.1"
                     /translation="MWILVPIITIVLLIIAVSSMQYILVMIAFLLIIYSFIEKKIVMG
                     LVSVLFFTYSIYLCATWEDKSLIADNKVETVRAQRETVEREKEMERRRIQEEVDKERY
                     IEKHGMEISENDLKVKLEALVPQEYKGKKYELKVGKFKRYSMYFDLTVQNEKFSNSEE
                     CKKFVKEIANDLKKIKISKAYFKFHSKDDGGIYNSVYIDYFRNIQNNVDNVENLEFNE
                     FELKTEEEEKREQEKIEQEKNSYNNYIQNRVVDPLDRIKKLKELLDSGAITQEEYNKK
                     KKELLE"
     repeat_region   3339..4154
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3339..3367
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            4309..4497
                     /gene="srl"
                     /locus_tag="ACGI68_003252"
     CDS             4309..4497
                     /gene="srl"
                     /locus_tag="ACGI68_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor Srl"
                     /protein_id="HFH0139673.1"
                     /translation="MNKIATKSRAEYMKNCRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4617..4982
                     /locus_tag="ACGI68_003253"
     CDS             4617..4982
                     /locus_tag="ACGI68_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFH0139674.1"
                     /translation="MKNELMMFEGKEIEVFEFEGRILFNSKDVANCLDIKNVNENIIL
                     MNEKQVVKLRNSDISNTDIRKLNNAGENFLTESGVYKLIFKSRKEEAERFQDWISDEV
                     LPSIRQTGAYITNNVIPKS"
     gene            5035..5430
                     /locus_tag="ACGI68_003254"
     CDS             5035..5430
                     /locus_tag="ACGI68_003254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139675.1"
                     /translation="MLKELLDDAGFDNKSKLLTAKTLYKKAEIDLPIEINEEEHYFDT
                     KQIASKLKIYSKSNKPAQMAVCEIIKKIDLEDGEVKGVWEINGSWTGTVNKYTKSVID
                     KVRTWIEENNRPTKIAGEKKNYYVFYKIE"
     gene            5482..5643
                     /locus_tag="ACGI68_003255"
     CDS             5482..5643
                     /locus_tag="ACGI68_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139676.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5971..6435)
                     /locus_tag="ACGI68_003256"
     CDS             complement(5971..6435)
                     /locus_tag="ACGI68_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139677.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKNKILRERKYKKRR"
     gene            complement(6571..6888)
                     /locus_tag="ACGI68_003257"
     CDS             complement(6571..6888)
                     /locus_tag="ACGI68_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139678.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(7171..7467)
                     /locus_tag="ACGI68_003258"
     CDS             complement(7171..7467)
                     /locus_tag="ACGI68_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139679.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKNYLNRF"
     gene            complement(7486..7899)
                     /locus_tag="ACGI68_003259"
     CDS             complement(7486..7899)
                     /locus_tag="ACGI68_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139680.1"
                     /translation="MKVKSKRSFIVGIIVCVLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7883..8116)
                     /locus_tag="ACGI68_003260"
     CDS             complement(7883..8116)
                     /locus_tag="ACGI68_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139681.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            8335..8709
                     /locus_tag="ACGI68_003261"
     CDS             8335..8709
                     /locus_tag="ACGI68_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139682.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEECFKNLK"
BASE COUNT         3566 a          929 c         1413 g         2948 t
ORIGIN      
        1 acttgctaaa ttacaaagag aattatcaag aaaaacaatc ggtagtttaa atagaaataa
       61 agcaagatta aaagttgcaa gacttcaaga acatatagct aatcaaagaa atgattttct
      121 acaaaaacta tctactaaat taataaaaga gaacgatatt atttgcatag aagatttaca
      181 agtaaagaat atgattagga atcgtaaact atctcgctca atttctgatg tgtcatggtc
      241 agagtttatt cgtcaattag aatacaaggc taattggcat ggtagacaaa ttgtaaaggt
      301 gggtaaattc tttgcaagtt cacaaatatg caataaatgt gggtataaaa acgaggaagt
      361 gaaggactta aatataagag aatggattta tcctagttgt aatgaaactc acgatagaga
      421 tataaatgca agtataaata tattaaaaga aggattaaga ctaataacaa ttcaaaataa
      481 ataacgtatg taagaaccgt aggaactacg gggatagcct gtggagaatt agtaagacat
      541 atgttagtat gcaaaattct gtgaagcagg aaacctgtga ctttagtcat gggaggttca
      601 ggctagtatt actcattatt gtattcaaaa gctaaagtgg aaacctagtg aatatatgaa
      661 tttagaagtt aatgagagag cattagcagc cgactcaata cttattaaaa ttgaagatga
      721 agaagaaaca atgaaagaag ctgaaagaga gaaaaagaga ggtagaagaa ggtagaattt
      781 aaagttaaaa tagttacatt gctaatatat gaaaatgtta caaatttgga aatagatgga
      841 tatatatgtt gtatagtata atgtagataa tatatcacca agtaaaaaac aacaaataaa
      901 tctaaattat gaggggtgga aaataaagat attaaactta caatatgtat atttgctttt
      961 gtaattggaa taatagtgtt attttgctgt ataggtctag gtcaatttaa tatggattgt
     1021 attataaaac aaaatggtgg gtcaatagct accgaacaat acaacatata tttagaacaa
     1081 tctatttcac aatatagaaa ttttggttct atactggttt tactaggagg aataggaata
     1141 ctgtttgata aaagtgtcag aaaatactaa aaaagtatat ttttatttag gatgtgatga
     1201 atgatatggc aagagatgta tttagtgtaa agatgacacc actagaagca cagaaaatgg
     1261 tagaaaaaaa tttggaagca gatttagttt ctgttgatat ttataaccta gaaaataata
     1321 agtctataat agttacaatc tttgaaaaat attatgcaag aagcaatagt gatgcaggtt
     1381 tggtagtgat ttgtgaaaat actacaggaa aaacacttgt taaaattacc tcaactggta
     1441 gtgcaattgg actatttcag attgattggg gaacaggtaa taatttgatt aaaagagtaa
     1501 aaaaaatact tattgattat attatttaat tattttatgt agatattctt aggttggtat
     1561 ggaatttgta ctaacctatt aagtaatcaa taaaactaaa taaacataga aagcacttac
     1621 aaatatgtag gtgcttttta ttgaaaaaat agaaaataaa ataaaaagta agatatagga
     1681 aaaatatgta agaattatat gttataataa ttgtagcaag aagatgtaat ctacaattta
     1741 tagagtggag ttcatgtatt aaaaaattat cctcccaacg ataagaaggg aggtgaatat
     1801 acatggataa ttttttattt aatgtgttag ctagtgtaac agctagttac atagtttatt
     1861 tgattagcaa gttaatcaaa aaagtaaaaa gccactctac cgcaaagagt gacttaaaga
     1921 ttgatataaa ttttaaatat catcgtaaaa aataactttc agttatagaa cttcactcta
     1981 ccgcaaatag attgtagttc ttcttgtttt tattatacca caaattagaa aaaatattgc
     2041 atataatatt ttttatagtc aataaaaata tgaaattttt tatacacaat gatgatgaat
     2101 ctaactagat gaataaattt aattgtaaat aagaaagtac ttacttaaat ggtaggtgct
     2161 tttgttttgc caaattatgc tataatattg agtgagatga gtaatttgtg ggggaatttt
     2221 ataatgtgga tattagtacc aataataaca atagtattac ttataatagc tgtttcttct
     2281 atgcaatata ttttagttat gatagctttt ttgttgatta tatattcttt tatagaaaag
     2341 aaaatagtta tgggtcttgt gtcagttctt tttttcactt attcaattta tctttgtgca
     2401 acttgggaag ataaatcctt gatagcagat aataaagttg agactgttag ggcacaaaga
     2461 gaaactgtag aaagagaaaa agaaatggaa agaagaagaa tacaagaaga agtagacaaa
     2521 gaaagatata ttgaaaaaca tggaatggaa atatcagaaa atgatttaaa agttaagcta
     2581 gaggctttag tacctcaaga atataagggg aagaaatatg aattaaaagt aggaaaattt
     2641 aaaagatata gtatgtattt tgatttaact gtacaaaatg aaaaatttag taattctgaa
     2701 gaatgtaaaa aatttgtaaa agaaattgct aatgatctta aaaaaattaa aataagcaag
     2761 gcgtacttta agtttcattc aaaagatgac ggcggtatat ataattctgt ttatattgat
     2821 tattttagaa acatacaaaa taatgtagat aatgttgaaa atttagaatt caatgagttt
     2881 gaacttaaaa ccgaagaaga agagaagcga gaacaagaga aaatagaaca agaaaaaaac
     2941 agttataata actacattca aaacagagtg gttgacccac tagatagaat aaagaaacta
     3001 aaagagttgc tagattcagg agcaattaca caggaagaat ataataaaaa gaaaaaagaa
     3061 ttattagaat agataatata gtaagcactt acaagtatgt aggtgctttt aaatttacaa
     3121 agtattccat tttaatttta tagtttagat tttatgatat aataaaaata tagaagtttt
     3181 gcagtgtgcg atatttgtta caaagtaggg cttaatactt gaaatctaag atgttgaggg
     3241 tgcgtgataa gtgttatcaa ttgcactatt gcccgctcac tgcaatttta agagtattgt
     3301 atatatgtag gtattggaaa tgctaagttt attttggggt tttagattaa ctatatggaa
     3361 tgtaaatatt acatgggtaa aaattttgat gatttttatg acagttttag attaactata
     3421 tggaatgtaa attgtataga attaagaatt tttttaatac taattatagt tttagattaa
     3481 ctatatggaa tgtaaatatt acatttaatt tgcattgctt tgatgacttc tttgttttag
     3541 attaactata tggaatgtaa atgcgtacaa ggttgagaag ctccgcaatc tcaacgtcgt
     3601 gttttagatt aactatatgg aatgtaaata tttgaaagga ataaatttta aatgaatgaa
     3661 aggataggtt ttagattaac tatatggaat gtaaatcagc caaaaacttc gaatttgaac
     3721 taaaatgcgt gggttttaga ttaactatat ggaatgtaaa ttctcatgac tattgaataa
     3781 atataataaa caagcatacg ttttagatta actatatgga atgtaaattt agaatcaaaa
     3841 gcacttaatc ctagttcttt catagtttta gattaactat atggaatgta aatagctttt
     3901 tagcttcgtc tactacttta taagcaaagc gttttagatt aactatatgg aatgtaaatg
     3961 atgaacaata aaacaatcat ctaaagacga agaaagtttt agattaacta tatggaatgt
     4021 aaatgttcct gttgtgtttt ttactaatcc cattttatat gttttagatt aactatatgg
     4081 aatgtaaatt gctgtgattt cactaataaa acaattaata attgtgtttt agattaacta
     4141 tgtggattca aaattaaata aacaaagaaa gcacttacaa atatgtaggt gcttttattc
     4201 tgctcaaaat tggtcggttg ggtaaaatca ttagaaaaaa ttagtaaaaa cctcttttct
     4261 gtaactcgtt acaatattat tattaatgta acgagttaca gaaaagaggt gaataaaata
     4321 gcaactaaaa gtagggcaga gtatatgaaa aattgtcgaa aagataaaag aggttttagt
     4381 gtacttttag acaaagaaaa gttagataaa tttgatgaag tattagagga aaagaatcta
     4441 accaagaaag aatggctaga agaaaaaatc gacgaggaac tggaacaaaa ggaataaaaa
     4501 ataagggtca ctcccaccga ccaaagtttg agtaacccct atgatgtata ctatcgtata
     4561 tcaattataa tatatgtcat tccttaaaaa aatcaattat taaggagtgt aatattatga
     4621 aaaatgaatt aatgatgttt gaaggaaaag agattgaagt atttgaattt gaaggaagaa
     4681 ttttattcaa ctcaaaagat gtagcaaatt gtttagacat aaaaaatgtt aatgagaata
     4741 taatacttat gaatgaaaag caagttgtaa agttaagaaa ttcggatatc agtaatactg
     4801 acatccgaaa acttaataat gctggagaaa attttcttac tgaaagtgga gtgtataaat
     4861 taatctttaa atcaagaaaa gaagaagctg agagattcca agactggata agcgatgaag
     4921 ttttaccaag cataagacaa actggtgcat acataactaa taatgtaatc ccgaaaagtt
     4981 gagagaaaaa gcaagtgaga ttgaaaaatt acagttgctt ataacagtac atctatgtta
     5041 aaagaattgc tagatgatgc aggctttgac aataaatcaa aactattaac agctaaaaca
     5101 ttatataaga aagcagaaat tgatttacca atagagataa acgaagaaga acattatttt
     5161 gatacaaaac aaatagcatc taaactgaaa atatattcta agagtaataa accagctcag
     5221 atggctgttt gtgagattat taaaaagatt gatttagaag atggtgaagt taaaggtgtt
     5281 tgggaaatta atggttcttg gactggtact gtaaataaat atacaaagag tgtaatagat
     5341 aaggtgagaa cttggataga ggaaaataat agacctacca agattgcagg tgagaagaag
     5401 aattattatg tattttacaa aattgagtaa atttatcagt tgtattaaat aatttagttt
     5461 agttttgggg ggattaatac aatgtgtgag aatttacttg ataatatgta tattgaaaag
     5521 agaaaagaag aatatagaat taaacttttg aaaataagag aaacagatat agatatatat
     5581 aataagttag aaagtatagt atataaactt tctgagaaaa aattagagaa aaataataaa
     5641 tagacatata aagcacttgg atattctact gtttcaagtg cttgtttggt ataaaatggt
     5701 ataatggaga tagagaatta tattaaccaa aaaatggaga aaatagagaa gatgatttag
     5761 ttgacgcttt atcagtagga gtacctgcag gtaagacaga atctctagta cgattagttg
     5821 gagatggaat aagtgatact caaaaaagtt taataaataa aagagaattt agaaatataa
     5881 ctcaaattgg tggaaatgga aatgagaaag catttagtga agtagaaaac ttagttaaat
     5941 agattagaag gtagcaacta gagttggttg ttaccttctt tttttatact ttctctctct
     6001 aagtatttta tttttcatat cttctaatac atctaatgca gtagtataaa attgtatatt
     6061 atttctataa ggtatagcta ttggaagaat gcccttgtta actaaaaaaa taagaagtac
     6121 aataaataaa agtgtcataa tcattctaat aaaataatta gtgatatctg ataaatcaaa
     6181 tgttaagtta attgttgtag aaaatacaac agccataata gatatataca aagcaaaaat
     6241 ggggttacta tttttttgta actgtccttt aagcctagat tgctctattt ctatatcttg
     6301 aatacttttt atggaatcca tttcactata atacttataa acttttaaat aatatttttt
     6361 atacttgttt aaagcttctt cattatcttt aaggtctttg tcacttaact ctgatttaac
     6421 ttccccaaaa tccataaaat ccctcctaca atgttatata atatacttta atttatagca
     6481 tatttaagaa cttatatcag caatttgtcg aacgatttta gctaaaaata gagtataatg
     6541 ataggcttat tatttataag atattatttt ttatatagtt atatatttct ttctaattag
     6601 ataatataat gctgttgtta taatggctac taatattatt gctattattg taatatctaa
     6661 tcttcttgta aataagccta ttgctatata ctcaagacct gttacatagc gataaagtac
     6721 ttctagttta acagtttttt tcttatctat atttccatat attgattcta atttattaaa
     6781 atatttttta ctacaaaaag cagttttagg tttaattaaa tatattattg tagttcctat
     6841 tataactatt attccaataa gtataagatt gatatttcca aaatccatag cagatacctc
     6901 ctattaaatt tgcttttctt aatcaaagta tatcagtaac acaagtacaa agagtatcta
     6961 aaatattaca aatattaaca ggatagataa aactatagta aaaattgtaa taataaagcg
     7021 aacgaaaatt atcatactaa aacataagac atgttataat tgtattagat agaagattaa
     7081 catacaccaa agcactcctt ataataaaga gtgcttattt ttttgaaatt catcaatata
     7141 taaactatca agaaccttat tcaacacacc ttaaaatcga tttaaatagt tctttttgat
     7201 aacataattt atatgatata ataaaaaaat agaagtgtac caccaataca cttctatagc
     7261 tataactaag cattctcttg ctgggggagt gcttttttca tttctccaaa caacattatt
     7321 tacttcatca gtaattatca aatcaatctc attatctttc aaaatctctg ttgcagtaca
     7381 aattagatta tctaagtcaa aacttcgagt tatctctagt tgtttgttat ttacttctct
     7441 ttttagtata taagttttaa aatacattta tatcacccct tgtatttact cttttttctc
     7501 taaaaatata ttgataagta aatatgcaac aaataaaaaa atctcaatga cacatagtgt
     7561 tatagctata actagaagtg attgattttt ccatgcacta taagctatga tgaaaagaaa
     7621 tgtaaaagta aataatgtat aattcataat tttaactaag atatgacttg ttttcattgt
     7681 taagtacaag tctctttcat ctgtactatc gtgtaattct tcaacaatgc cttttcttga
     7741 aaaagcatta caaaaattaa agattgctat tacaattaaa agaaatgatg aaattaggaa
     7801 tcttttatct tttaaaatgc aataaataac taaactagca caacataaca cacaaactat
     7861 aatacccact ataaaacttc gtttactctt tactttcatg ttttttatcc tccatttctt
     7921 tattttcttt taaacaacat agttcttcaa tcgttgtacc aaacacttgt gccatacgat
     7981 aggcaagcat aagagaaggg ctatattgct ctttttcaat agaaataatt gttctgtttg
     8041 atacatggac taaatcagcg agttgttgct gtgttagacc catatttgtt cgaaattctc
     8101 gtacttttgt tttcattcca taacctcttt ctatatgaag ctaacttcat gtgaagttaa
     8161 cttcataata tcctaaaatg agtaaaatgt caatctcatt agataagtat tataaaacct
     8221 aaaagaataa tatggtaaaa attggatgga attatttacc ttaattctct tgtttgtaaa
     8281 actttattga tatagaattt aacatataca taaatcggca aaggggcgcg aattatgaaa
     8341 attagtaaat taccagaagc ggaattaaaa gttatgagat atatttgtga agctaacaag
     8401 gtattaactt ctagggaatt agttgctgca atggaagaaa aatatgagtg gaatgaatca
     8461 actacattta cagttttgaa aagattagag cgaagagagt ttttaagtac agagaaaata
     8521 ggcaaactta cacattataa tatactagta aaagagaaaa aatatctaag gtttgaaaca
     8581 aaggaatttt tgaagaacat acataaaaat tcaatttcaa gtttaatgtc agcgttacat
     8641 ggtgaagacg acgaagtgga tgaagataaa ttaagtgaac tcgaagaatg ttttaagaat
     8701 ttgaaataag aaaatgacaa attgatacat aataaataaa atgaaggtat gaaattataa
     8761 ctttttctac cttcattttg tttattattc tgaacatgtt aagaaaatga aataagtttg
     8821 tattttagtt atataaattt taatagaata tgtaca
//
LOCUS       DAYASV010000150         8769 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYASV010000150 DAYASV010000000
VERSION     DAYASV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.155"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(98..430)
                     /locus_tag="ACGI68_003262"
     CDS             complement(98..430)
                     /locus_tag="ACGI68_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0139683.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            696..1316
                     /locus_tag="ACGI68_003263"
     CDS             696..1316
                     /locus_tag="ACGI68_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0139684.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(1641..2465)
                     /locus_tag="ACGI68_003264"
     CDS             complement(1641..2465)
                     /locus_tag="ACGI68_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HFH0139685.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNT
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(2476..3879)
                     /locus_tag="ACGI68_003265"
     CDS             complement(2476..3879)
                     /locus_tag="ACGI68_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139686.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDDGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(3883..4608)
                     /locus_tag="ACGI68_003266"
     CDS             complement(3883..4608)
                     /locus_tag="ACGI68_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139687.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(4720..5493)
                     /locus_tag="ACGI68_003267"
     CDS             complement(4720..5493)
                     /locus_tag="ACGI68_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139688.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFMIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(5499..6386)
                     /locus_tag="ACGI68_003268"
     CDS             complement(5499..6386)
                     /locus_tag="ACGI68_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139689.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSVVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(6400..7422)
                     /locus_tag="ACGI68_003269"
     CDS             complement(6400..7422)
                     /locus_tag="ACGI68_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139690.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDVGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALK
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            7997..8173
                     /locus_tag="ACGI68_003270"
     CDS             7997..8173
                     /locus_tag="ACGI68_003270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139691.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT         3118 a         1347 c          869 g         3435 t
ORIGIN      
        1 aattaaaaat actaactatt aatttaaata tagactgtga gtagattatt caaacaattt
       61 tactcacagt ctttttatgg tatctacaga tacaaaatta aggtctattc ctctctcccc
      121 actcacacat tgcatctaaa attggaatca gagattttcc cctttctgat agactatatt
      181 ctaccttagg aggtatttga ggatattcag tacgaataat caagttatct gcttctaatt
      241 cttttaatga taaactcaaa gttttatagg agatagtatt tatataacgt tttaatgcat
      301 tatatcttac aacttcaaat tctacaagac agtaaagaat tatcatctta tatttaccac
      361 taatcagtga aagtgtataa ctaaatcctg tttcacttaa atctgtattt gatatacagt
      421 ctatactcat atattacact ctccttttgg ttagtattga acaaaaatgt gcgtacttgt
      481 ttttagaata atttataata aaattatagt gtaagaataa ttgaaagtca attttataat
      541 aggaggtgta tttactaaaa aaaaagttat tttaaatagg attctaagat tagacatttc
      601 tatataaatt gataatattc atgaaaatcc tattttataa aaaataatgt ctcttagaaa
      661 atctattaaa taaaattcat tggaggaaat ttataatgaa agttttattg ataaacggta
      721 gcccaaacca atatggatgt acttatacag cactaaatga gattactaaa gtattgagta
      781 aacacgatat taaaacagaa atactgtatt taggtaaaga aacaatccca ggatgtatta
      841 gttgcgcatc ctgttttgaa actggaaaat gtattcgaaa tgataaagtt aatgaattga
      901 ttgaagatct ggataatatt gatggtatta ttattggttc tcctgtttac ttttctagtg
      961 caactggtca attaacttct tttcttgacc gtctattttt catagctggg agtcgcatgg
     1021 ctacaaaatt aggtgcttca gttgtgtcat gtagacgtgg tggtgcaagt gcaacatttg
     1081 accaactaaa taaatatttt tcaatcagca atatgcctat tgtatcatca cagtattgga
     1141 accaggtaca tggctttact ccagaggatg ttatgaagga tgaagaagga ttacagacta
     1201 tgcgaactct aggagaaaac atggcatggc ttcttaagtg tatatcagca ggtaaaaaag
     1261 ctggaattaa agaaccacaa tatgaagaaa caattatgac taattttata aaataaataa
     1321 aaaatgtact aatattaatt aaaacaaaac actaatttat aaataaaata agcataaatg
     1381 taggataaaa agcactaaaa cagaggtatt gaattttata tcaaacctct gttttattat
     1441 tacattaata accaaaccat gtataacatc atatatttct aaataaatta taatattata
     1501 ttctaatgtt attgaaatta caataactga atttctgctc ataaatttaa atattctaca
     1561 aatctcatct aaataacaat tttaatagac ctaaaacaat cctcatactg ttcattttgt
     1621 attaaaaata tcacttacaa tcaaaaacca tttattaagt atattcctaa gatatacaat
     1681 atatatacat ggattggata gtataaataa aataaatact tcatactctt ccctttttct
     1741 ccattgtaaa gtaacattaa aggtagagaa gcaatcatca tccattggta attttgtaaa
     1801 atagaatttt ttaaactata atctccattt gacattgttg taaatataaa tatacacatt
     1861 aaaacataac aaatactcaa actgaacttt ttacctctac acaaataaaa aataatccca
     1921 agtataatcc aaattggtcc accttctact aaaaatggta ctggtaccaa aatcataaat
     1981 aaagcaataa ctctgttagt gactttaaaa agtaaagcta ctgtaaatat tcctaaaatt
     2041 ataggaatga ttatcatcaa taatcctata ataaaatatt taatttcttt ctcttttcta
     2101 aatttcttta tgaactctat tgcttgtaaa taaatcgcta ttaaaaataa tgttgaaaaa
     2161 gtgttattta taattatagc tccttctgga tgtgaaaata cattattaat tatttgattt
     2221 cctatagcca taataacaga ccaaatatac aatctactta tatatttttt tctgttacta
     2281 gtgtgataaa atccttcaac agccatgaat ataaacagtg gtgctgatat tctgcctacc
     2341 caatgaaacc aaattggtat ttgcattgtt tgtggcataa atgctgctat atggtcaaat
     2401 gtcataaata ttaatgctaa aatcttaata gtaaacccac ttataccttt tttaaaaaaa
     2461 tccatatcat atctcctaat tttgttttat ctctataata atttttgttc ctattccaat
     2521 ttcactactt acataaattt tacctttatg aatctctatt atttctttag aaatagccat
     2581 accaagacct gaacctttat gcatctcacc tgtattagta cctctataat attttttaaa
     2641 aatatgtttc aaatcacttt tgcttattcc aattccatca tcttttatta taatttctat
     2701 attatctttt tttacaattt ctacacttat caaagttcct tcgctattat gaactattga
     2761 attaaatata agattggtta tagcccttct gaataagatt gaatctacat atacttcaat
     2821 taaatcttca ttactttcaa attcaatatt cctattttta tatctattat catttaaaat
     2881 atcaattatt atatctctga ctaaactgac taaatttatt tttttcttgt ccagaactat
     2941 agtattattt tttaatcgag tagataaatt taaatcatca actaaatctt ttatatactt
     3001 agacttatta tatataattt ctgtatactc ttgtatttca tcttcttcaa actcataatc
     3061 tttgtcatta attatctctg catacccttg tatagatgct agaggtgtct ttatatcatg
     3121 tgttatattt gccaaccatt cctctctcaa ctcttcattt tcttttctct caacttcatt
     3181 tactcttaaa gtatcagcta acatatttat atttttaaaa acttcttcat atatacctct
     3241 gtccttaaga tataaactat aatttccatt agccatagtc tcaatactcc atagtacgct
     3301 tgaaattggt tttgtaagtt ttctactaaa taaatatcct acacctaaag ctaccacgct
     3361 atcaattact agtgttataa tgataacttt atttaaagtt tttttaatgt tattttgaga
     3421 ataggttaaa attattcttg atatatctct actaggtata cctactatta tgtttagatg
     3481 ttttttgttt aaatattttt ctaatacaaa attaacatac tttgtttcta tatacttgta
     3541 attattcacc atttgaaagg gtgtatattt tttgggagta tcctttggca catttactcc
     3601 ataaacttcc tctcctaaat catttaaaac ctgtatccag gaatttgatt tttctaattt
     3661 ttcagctcca atcttagata gtttaaattc tccatctttt tcatataaat ctttttcaaa
     3721 agaacgtgca aattcttcag gattatttcc agaatctaca actttaaaaa aactattagt
     3781 tgaaatcaca tacaatatag atatgatatt tataattact actgatatag ctacaaacac
     3841 aattgtaaat ataaaatttc tagtaatctt ccacttcata ttctaatctt cctttactgc
     3901 taatttatat ccaagacctt ttattgtcag aatatattca ggctttgaag gattatcctc
     3961 taatttttcc ctaatttttc ttatatgaac cattatagta ttgtcaaatc caacataatc
     4021 ttctccccac actttatccc atagtttttc tttacttata atttgatttt gattatgtgc
     4081 aaataaagat aggattttta gttcctttgc agacagtcct atagattttc catcttttat
     4141 aagttctgcc ctactttcat ttaatatata tgggccaaat gatataattt tttcttcatt
     4201 attatttaga tttttacttt cgttttgagc atcactaaaa taacttaatc ttctcaaatg
     4261 cgctttaact ctaaaagcca cttccttagg gctaaaaggt tttgtaacat aatcatctcc
     4321 tccaatcgct agtccaagta acttatctaa ttcttcggtt ttagctgata aaaatattat
     4381 tggagaattg gaggtttttc ttatttcttt acaaatatca taaccttcgc catctggtaa
     4441 cataatatcc aatacaatca aatcaggatt aatactttta aataattcta atccttcttt
     4501 tttagttttt gcagtatata tattattaag gccctctttt tttaggacag tttctaaaag
     4561 cttcaatatt tctacttcat catctattat taaaacacga ttttccataa acaacctcct
     4621 gtaataattt acatatatat tagcatatat tttatctaaa attcatttat cttattttta
     4681 attaaactaa aatataaata aaattactat tatttttagt taaaaccttt tgcttgatgt
     4741 aaaattttca tatgttattt tagttaacac tatcattaat ataattgaga ttactataga
     4801 aaaaactatc aaaatacttg gtacattata cttaaccatt tgttggctca tcataatcca
     4861 atttggagtc actgacataa attttatagc tttatctggt ataaaaccta cactaaagta
     4921 tggaattgcc attatcaatc cacctagtat aaatggaatt acagccgatt taaatataga
     4981 tgaaagtgtt aagcctattt gtgcaaatat aaaagaagca aaaatagtag tcaatataac
     5041 aattatatac atttgtatta ctgtaagatt taaattacta tttccaaagt accataagtt
     5101 atgaatcgat tcatttaaat ttttaaaatc aaaaaaacta aaacctctaa ttcctactaa
     5161 caatataaaa gaaatattaa taatagataa aattaatcca ttagctacta gttttgataa
     5221 tactactgat ttttttccct ttttagttgt atttattaaa cctgtcatat tagaatcata
     5281 ttctttaatg taatttccag aagttattat aaacattatc aaacctatta gaggtattga
     5341 gccttctatc ttgtaaaata tatctgcata agtatctaaa acatcaaact ttctaatcat
     5401 aaaaaatcca aaaaatatta aaaacagcca aattatccaa acactctttg attttaatat
     5461 ttttatcatt tcagctttaa ctaaatacat catttctact acacctccct tgcatcttct
     5521 aaatcaaaat aaaacatgta tacatcttcg aattttgctt gaactggttg tatattatcc
     5581 tcgcttggaa tatcctctga aacaattcta agtactactg aatcaatatc actttgaata
     5641 cttacaacct tatatttaga ttgtatatca tcaacttcac attcattttt aacttgaaca
     5701 ttatatactt tattttccat gtgacttaat atgtctttgt gtgttccttt cattaggagt
     5761 ttgccatctt taatcattat tgtttccttt gcaactgatt ctatatcgga aattatatga
     5821 gtagaaagta ttattatctt atctctagac atttttgcta ttaaattcct aaatctagct
     5881 ctttcttgtg ggtcaagtcc tgctgttggc tcatcaagga ctattatctt agggtcgttt
     5941 agtaaaactt gtgctatccc aactctcctc ttcatacctc ctgaaaattt tcctatagac
     6001 ttatttctca catcataaag acctactaaa tctaataatt ctcctatctt actatttaaa
     6061 tattttcccc ttaaaccttt taaagctcca atatactgta aaaattcttt tgctgtaaaa
     6121 tttttataaa ctccaaattc ttgtggcaaa tatcctaaaa tatttctata atcagcatcc
     6181 attttaaata tatcctcacc attataaagt atctgacctt catcaggttt tatcaacgtt
     6241 gttatttgtt tcattaatgt agattttcct gctccatttg gtcctagaag accataaact
     6301 ccattttcca gagttactgt aatcttgtcg tttgctactt ttttaccata tatctttgaa
     6361 agattttcta tttctaattt attcatctta actccccttt tatctactta tataatctga
     6421 ttttattact aaaaaacata atgacatagc acctatcaat attgccatca aaagtcctat
     6481 tgctatattg acaatgtcta tattcacttt aaatatcata atatcaccta gataatttcc
     6541 tattatttta ttaaaaccaa atatcaaatc ataataattt acatttgata caactgaaag
     6601 taatttttta ggtaatatat ggattaccga cattatctta gttatagata taataaatat
     6661 aaaaaaactt attgaagata ttgaagactt acttataaaa gacccaaata tggctatcat
     6721 ggcccatcct tcaaacatca aaagctctat gcctatattt aatattacat actttgttat
     6781 acttaaatta gttttcaatg caactacatt gtctactatt ctaaaagatt ctaagtctcc
     6841 attaacaggt actccttgtt gcatacaagt tataataaat attcccatta aataaacaga
     6901 ataaattact aatggaacta ataaagcaac aaatatttta gactttaatg cttttttctt
     6961 ctcttttgat gatagtatga tatcttttac cgaagtaacg acttcatcta cataaatatt
     7021 ggatataatt atagatataa tgattaacat tcctacattt ataagtgaat ttgtgactct
     7081 atagttaaat actttccaaa aactaatatc cttatattca ttcacactta gattatcaag
     7141 ttttttcttt gacattaatt caattttttt agaaaattca cctgtatctg tttgatttga
     7201 aaactgcttt aaaacattaa tcttattttg aaatttctca ttacctacgt cgataccact
     7261 tctcgtatct tctatacgtc ctttttcttt atctatataa taactttctg tttctaaaac
     7321 aggcgtaata aagatactaa tcattagtat taacactaat atccctagag atataaacat
     7381 actcttagat ttagccaatt tttttatttc ccaatttacc aatatatttc tcctcctaaa
     7441 tcattttaat tacacatata tattaaaact taaatcttat ataaatataa tactaacctt
     7501 aaataaagct taaattattt tataatttat aggtgtgttt attattggag ttacatttat
     7561 tatttttctg ggaaattata cattttcaat tatttttatt atttttgata tttttattga
     7621 ttggaaattt tattgttgtt caaattgtag ttattcttta aatttaaaga tggagttttt
     7681 taagaataga acctactgtc cttattatgg aaagattatt aaatagttgc atataacttt
     7741 atttatcttg aatcttattt ttaaaaacaa ttataatttt agaatatttt ctattctcaa
     7801 acaagcatgt tgaataatgg agcatcttct agtattatat acttgtagaa taaaactaaa
     7861 ttggtaaaac tgggaatatt taaccttaag gagttaagtt atagaaacta gtactaaaaa
     7921 attttataaa ttatgttaat tgactaatag aatgcgactt tttattttta ctaaattttt
     7981 tagggggatg gataatatgt ttattggaga aatagacaaa tgtacacata tactaactgc
     8041 ttacatcagt agttcatatg attattgtaa ttttttagat acacagttag atgattttat
     8101 atcagaatat ggagaaacgg tagttgaaac ctgcttacat caagtcttat tattagtaag
     8161 tagatacaat taaaactaaa tttttctaga aaaacctttt tagatttatc tactctaata
     8221 aatatataat tttttgtact ttataagtaa ctataacttc tgctaaagta aagatgtatt
     8281 gggagtacat ctttactttc ccattataga agttaaaagc atacaccata aaattaatca
     8341 tccaatacaa attatatatt tcctatgtct aacttaattt acaagaatca ttctatatgc
     8401 atattctgca atccactaaa catggctcta aacctttact attttataca ttctctataa
     8461 atcatacaaa tcatatataa aatcccccta atatgtttaa accacctata aaataacacc
     8521 aatatttaga atattcaata tcaactgcat aactggtcaa taaaaacgta taactagaca
     8581 aatttcaata tttatataaa taatacaaga aaaacaagca ttttatttat ataagttttt
     8641 tattttttgt ttatattaaa aaactatatt gattaattta ttttatttag taattataaa
     8701 aattcgtctt agaatatatt ctatatttgt agattaactt taatattttt aatttcacaa
     8761 aaattaaac
//
LOCUS       DAYASV010000151         8553 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYASV010000151 DAYASV010000000
VERSION     DAYASV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.156"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            298..1284
                     /locus_tag="ACGI68_003271"
     CDS             298..1284
                     /locus_tag="ACGI68_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0139692.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKELKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1620..3101
                     /locus_tag="ACGI68_003272"
     CDS             1620..3101
                     /locus_tag="ACGI68_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFH0139693.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3127..4098
                     /locus_tag="ACGI68_003273"
     CDS             3127..4098
                     /locus_tag="ACGI68_003273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFH0139694.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4354..4956)
                     /locus_tag="ACGI68_003274"
     CDS             complement(4354..4956)
                     /locus_tag="ACGI68_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFH0139695.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPTLWAGKPVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILDNSIEVDNC"
     gene            5237..6448
                     /locus_tag="ACGI68_003275"
     CDS             5237..6448
                     /locus_tag="ACGI68_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFH0139696.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSECGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKYNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6516..6713
                     /locus_tag="ACGI68_003276"
     CDS             6516..6713
                     /locus_tag="ACGI68_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HFH0139697.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILSLYIKQLGVTDVSLI
                     QQYSGIVFGCTFVVAAFFSPI"
     gene            6747..7718
                     /locus_tag="ACGI68_003277"
     CDS             6747..7718
                     /locus_tag="ACGI68_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0139698.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFMNALWMYKFTGLFIKDKTK"
     gene            7905..8282
                     /locus_tag="ACGI68_003278"
     CDS             7905..8282
                     /locus_tag="ACGI68_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139699.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3479 a          889 c         1368 g         2817 t
ORIGIN      
        1 tttaagtgat aattaagtgt ttatcgataa ggctatggtt gtaaaaatca tagccttatt
       61 tttatattat aaataaatta gagtatttat caattcatag tagataaaat tgaaattaaa
      121 tataacaaca aaaaatgaaa attacaatta atttaatgtt aaagactcaa ttatttggat
      181 gtggaatgga tagtacaagt agtatataaa aagtattgac tggacaaata actatatttt
      241 attttaaata tataatataa tgtaataaaa tatatcaaat gtagaaggtg ataacctatg
      301 aaaaaaataa caataaatga tattgctaac ttggcaggag tatctaaaag tactgtatca
      361 agatatctaa ataataaaga tatcagtgat tctactaaag agaaaataaa gacaataata
      421 gatgaatatg gatatgagcc gaatgcattt gctcaaagtt tgagagctaa aaaaacatac
      481 tttattggta ttataacacc gtgtttagat tcttttgtaa aatctaaaat tatgatggca
      541 atagatgaag aactcaaaga attaaaatat acatcactaa ttattaatac taatagaaaa
      601 ataaggtcag aaatagatag tatatcaaaa ttagctagtc taaaagttga tggaataata
      661 ctaattggta ccgaaataac taaagaacat aagaatgaaa ttgaaaagtt agatataccg
      721 atagttgtag ttggacaaaa agttgatggt atcaatagca tagtgaatga tgattatgga
      781 gcaggatata aaatgggtca atatattgct aataaaggtt acaaaaacat tgtatattta
      841 ggtgttgatg aaagtgatat atcagttggt ttaaatagaa aaaatggagt acttaatggg
      901 ttaaaagata aagggtatga tgcaaaagta ttttatactg actttgacca agaaacgtct
      961 atacaaagaa gtggagaaat gctagaaagt gaaaatccag atataataat atgtgcaact
     1021 gataatatag caatagcaac aatgaaagaa atcaataaga gaggtaaaaa tataccacaa
     1081 gatatatctg tagctggatt tgggggatat gatgtattgt ctattataag ccctaaactt
     1141 acaacaataa agtttgaaaa taaaaatgca ggtaaagtag cagcaaatac gatagtaaat
     1201 ttaatacaag aaagaaaaga accactatta aaggagatta aatttgaatt aatagaaggt
     1261 gaaagtacaa taaataaaaa ttaagataca cattacatat attagtaatg tgtatttttt
     1321 tactaaaaaa actataattc taaaaattag gaaaactttt ttgaaagaaa tattgcatct
     1381 gaaataaatt tatgctaatc tttaattaaa ccggtttcga aaccggttta attaaaccga
     1441 tagaatagca aaatgagtgc aaaaataact gattgtaaca aagtatcaaa gtaattatta
     1501 gagtacatag gaataaacaa aatataaaag atgtagcaca ttgtgtaata agactaagaa
     1561 ttgttttaga tagcaataat aaagcaaata taaaattata aaagaaaagg aataaaaata
     1621 tgtacaaaga gcctaaatat agaaccatat tagaatcgac aaaagaagaa ttaaataaat
     1681 taagaaatat atctttaaat gataaatata aaccactatt tcatatacat ccacaacatg
     1741 gactacttaa tgacccaaat ggattagcat attataatgg taagtatcat gtattttatc
     1801 aatggtatcc atatgatgca actcatggaa tgaagcattg ggcttatgta agttctgatg
     1861 attttgtaaa ctggaataga gaagatgtag cattaatacc aatcgaaagt tatgaatctc
     1921 atggagcata ttcaggtaat gcgatagagg tagatggaaa attgcatatg tattatacag
     1981 ggaatataaa atatagtgca gaagatagat atgcatatca aaacttagct ataatgaaca
     2041 aggatggtaa aataactaaa tatgaaaata atccaatagt aagtgaaata ccaaaaggat
     2101 atacaggaca tgtaagagac ccaaaagtat ttaaaagaaa ggataaatac tttatgttac
     2161 ttggagcaca aacaagtgat aaaaaaggag ctataatagt atatgaatct aaaaactcta
     2221 tagattggaa ttttaaagga gaattaaatg taaaaaatat agatgaagat tttggttaca
     2281 tgtgggaatg tccagattat attaatatag atgaaaaaga tatattaata ttttcaccac
     2341 aaggagtaga acctaaagga tttgattatc aaaatatata taatgtagta tatgctattg
     2401 gtaatatgga tttagataat ttaacatttg aaatagatac aatgaaagaa ctggaaaaag
     2461 gatttgattt ctatgctcca caaacattta taaaggactc acaaataata ctatttgcat
     2521 gggctggaat gggagaagtt ttatacccta cagataaaaa caaatgggca cattgtctaa
     2581 ctgttccaag aaaattaaat ataaaaaata ataagctatt gcaaatgcca gtagatgaat
     2641 taattaaatt aagatatgat gaaacaagtg ggcaaaatac tataaagaat aatattaata
     2701 ttatagaaaa tgatgaaaat gtatatgagc ttaatattaa tataaaaaat attgacagca
     2761 ataaatttgg gctggaatta ttttcatcac aagatgaagg tgtaaaatta gaatttaaca
     2821 aattaggaaa cattgtaact ttagacagaa gcaactttaa aaaagttttc ggtgttgaat
     2881 atggaacaaa tagaaaagaa tatataaata ttgatgaaaa tactaatatc aaagtactag
     2941 cggatagaag tattttagaa atttttataa atgaaggaga agtagtattt actagtagaa
     3001 tatttgctaa agaaaattct aatcaaataa gggtttatag tgataaaata gtagagtatg
     3061 agtatactaa atttaaatta aagcaaggta ttgaattata aaaacacatt ataaggggta
     3121 gaaaagatga aaaaagtaat tagcatagga gaagctttaa tagactttat accgaatcaa
     3181 aatggtggta aattaaaaaa tgtatcagaa tttagaagag tagcaggagg agctccagca
     3241 aatgttagtg ctgttgtagc aaagcttgga ggaaaatcga gttttatatc taagttagga
     3301 aaagatgcat ttggtgatta tataatagat gtattaaatg aagtaaatgt aaatactgat
     3361 tatgtattga gaacaagtaa agcaaataca ggacttgctt ttgtatcatt aaaagaagat
     3421 ggtaatcgtg atttctcatt ttatagaaat ccaagtgcag atatgctttt agaagctgat
     3481 gaagttaaaa aagagtggtt taataattgt catatcctac atttttgttc agtagattta
     3541 atagatagtc caatgaaatt agcacataaa aaagcaatag aatacgcact tgaaagtaat
     3601 agtataataa gctttgaccc taatataaga ttacctttat gggatagtga gcaatcttgt
     3661 aaaaaggcaa tatcagaatt tttacctttt gctcatattg ttaagatatc tgatgaagaa
     3721 ttagaattta taacaggaga aaatgatata gaaaaatcat tacataaact ctttgtgggg
     3781 aatgttgaac tagtattata tacaaaagga aaagatggag tagatgcata cactaaaaaa
     3841 gtaaaaggta tgtgtaaagg ggttaaagta aatgcaatag atacaaccgg agcaggagat
     3901 tcttatatag gttcattttt atacacctta ttgtataaac aaataacatt gaatgatata
     3961 aaagaaatga aacaagaaac gttaaatgag tatttagaat tttctaatta ttatgcagct
     4021 cgtagtacaa ctattaaagg tgcgataagt tcatatgcaa caaaggaaga aataactgaa
     4081 tttataaaaa atttatagta ggaatgagta ataggttttg aaggaagtaa catgtaaaga
     4141 gtttttaagg atgtttacta tgaaaataat taacatataa aaagcaataa agaggctgtc
     4201 tcaaaataga gataagcctc tttatcattg ttgaaatgat aaaaaatatt ttaattattg
     4261 ttacttttta gaagaatata tatcttattt taaataagca aattatgtaa gatttattta
     4321 tattatcaat actttacttg gagtattatg tctttagcag ttatctactt caattgaatt
     4381 atctaaaata taataaattt gttttgcaaa ttgacgagaa tgttcttctt tttctttatt
     4441 cataaaatta ggtttaccta aatcacgttc aacaagcatt cctatatatt ttaactgtga
     4501 atgcttacag tagcgcttca taccattttc ccaaatatca gcaccctttt caggtctata
     4561 tccacatgtt gtaataagtg ctactggttt tcctgcccat agagtggggc ctttctcatt
     4621 accatagtat ttattcattc catacaccaa tctatcaagt agtgctttca ttggagaagt
     4681 gcagtaccaa gaataaattg gagtagcaaa aacaattaaa tcacacataa gaattttctt
     4741 gaaaattttc tgaacatcat ctttacaata acatccaaaa tcaaataagt tatcctgaca
     4801 ttttttacaa gcaatacatg gctctatttt acaatcatat aaccatatta cttcaatatt
     4861 tgaattataa ttttctaatt cctcaataaa aggttttaaa atagcagcag tattaccttt
     4921 tttcttagga cttcccataa gaatgcatac ttccatattg atacctccac agtattttaa
     4981 aatagttttt agtgcttaat atagttttag gatactatga gaatacgtta acaacaatac
     5041 actttgtata tttgtaataa aattgaaaca aattgtttta tcaataaaag ttggaatgta
     5101 tctataatta aaaatatttt aggtatgttt ttacatatat gtgtgtagtt aactatggta
     5161 atttattgta aaattatctt ataggattat aattgcttaa tttatgatta tatttaaaat
     5221 cttggaggta aaaaatatgt actcaaatga agactataaa tttatagaag aaaatgcacc
     5281 atgttatata tatgatgaga ctaatataat tgaaaaatgt aaggaattga aaaataatat
     5341 tttaggtttt gattttttat attctataaa aacaaatcca tataacaaaa ttgtaaaaag
     5401 cattgcagag caaggttttg gagcagatgc ttcttcaact aatgaagtta tgatttcatc
     5461 agaatgtgga gtttctaaag atgatattta ttattcatca ccaggcaaaa cagagattga
     5521 tatagaaact gttttagata aatgcataat tattgcagat agtattacag aaatagaact
     5581 tataaataag atatctaaaa agtataatca gaaagttaaa ataggaataa gaataaatcc
     5641 tgattttact atggatgatg atagtgctaa atcaagcaaa tttggtatag atatagaaga
     5701 tatgagcagt gtttttaaga tgttaagtat gtgtgattat gtatctgtag ttgggattca
     5761 tatacatatt aaatctcagg tattggattt tgaaaagtta ggacattatt atgaaaaatg
     5821 tttttttata gctttaaatt taaataaaag taagtatatt aatattgaat ttataaattt
     5881 tggtagtgga cttggagtag cttatgataa tcttagagaa aatccactta atcttaaaga
     5941 gctttcaaaa ttaactcaaa agattgcttt tactaattca aacacactaa aatcaagact
     6001 tcttattgaa tcaggacgtt tcatagtttg taaatcaggt aattattatg ttaaaattgt
     6061 agatattaaa gtatcaagag ggaaaaaata tcttattgta aaaaatggac ttaatggatt
     6121 tatgagacct attatttcta atttaattga aaatattact tgtggagtag aattagaagg
     6181 tcaagagcca ttatatacat caaatcatgc cttttctctt tcagtattaa ataatttaaa
     6241 agaagaagag tatgtaaatg ttgttgggaa tctctgtaca tcattagatg taattgcaaa
     6301 taatgttaaa ttgaaaaaag caaacatagg agatttgatt tcaattagta atgctggaag
     6361 ttactcctat tctttatcac cgctattatt ttcaagttat gaatttccta aacaatatta
     6421 taaaagaaaa gatggatata ttgagtaaac aatagcataa attaataaac ttagtattat
     6481 tgaaaaaatt taaaatgcag ggggtgtaat tctatatgga attgtggaaa agaaatttat
     6541 ttgtatgttg gataggtatg ttttttagtt ctatagggat gagccaaata gcacctatac
     6601 tttcattata tattaaacag ttgggggtaa ctgatgttag tttaatacaa cagtattcag
     6661 gtatagtttt tggatgtacc tttgtagtag cagcattctt ttcgccaatt tgagggaaag
     6721 ctgctgataa atatggaaga aaatcaatgc ttttacgtgc aagtattggg atgggaatag
     6781 ttatatttac catggcgttt gttcaaaatg tctatcagtt attaggatta agaatacttc
     6841 aaggagtttt tacgggttat gctactgcat gtactactct tattgcaaca cagacagata
     6901 aaaatcattc aggatgggct ttgggtactc ttgcaactgc atcaactact ggctcattaa
     6961 ttggtccaac agtaggagga tatatagaat caatcttagg tcttaaaagt acatttatta
     7021 taacaggagg attgttgttt gtatcattta tcatctctat tttatttgta gtagataatt
     7081 ttaaacctaa agaaataaaa gaatcaattt ctatacctaa agaacaatta aatatattgc
     7141 caaataaatt tttaattgca tctatttttg taaccacatt cattactcaa ctagctttat
     7201 actctataga accaattgta accatatata tatcacaact tacaaatttt gcatcaaatg
     7261 tagctttaat agctggattg acattttctg catcaggatt agcaaattta ttagcagctc
     7321 aaaaacttgg aaaaatgtcg gataaaatag gtcctcaaaa agtattgtta atttcacttt
     7381 tatgggctgg gattatattt ataccacaag cttttgtaaa aactgcatgg caactgatgt
     7441 tgttgagatt tttattaggg ttgtcagttg cagggcttaa tccttctgtt aacagcttgc
     7501 ttaaaaaaat tgcaccagaa gaatatgtgg gaaaaatatt tggatataat gcatctgccc
     7561 agtatatagg ctgttcatca ggagcatttt tagggggaca aatatcagca catttaggta
     7621 ttagaacagt atttttttca acaagtttat tgttgtttat gaatgcactt tggatgtata
     7681 aatttactgg attgtttatt aaagataaga ctaaataatc atactattta catttctaat
     7741 tttatgatgt aaaataaata ttttttatgt aatttatcat agaattatat aaaaaataac
     7801 cctatggtaa aaattggaaa atattattat cgacaaaatc tttgacttaa aaactatatt
     7861 gcgatagtat taatttaata aaagtttgag aggggcgtaa ggttatgaca atttcaaaaa
     7921 taccacaagc agaattaaaa gttatgaagt ttatttggga aaaaaatgat acagtaacat
     7981 caaaagaaac tattgaagct atggaacgaa aatatggttg gaaacaaaca actacattga
     8041 cacttttatc aagacttgtt aaaaaacatt ttttaagtgc agaaaaaata gatagataca
     8101 ctcattatac aatagttgtt ggacataagg agtatttaag tgttgagaca aaagattttt
     8161 taagcaatat acacgacaat tctttacaga gtttaatttc agcactacat gatgatgaag
     8221 ttgttgataa agatatgttg gatttatttg aaagtcattt taaaaattta gaagaagagt
     8281 aattatatca ggatagaact ctaagaattt aattaatcta tggctgaaaa ccataaatgg
     8341 tattatttaa tcagctactc actattcata aagtaatatt aaataggttc tctaattagt
     8401 aaaatgccta aaataattgg aatagggagt atcaagttaa atgataccac ttatttaaat
     8461 aagaaatgat ttttaaaatt taaaataatt ttataacttg tcaatattca ttaatctaaa
     8521 gtattatttc tatttaacca tatcaaaata tat
//
LOCUS       DAYASV010000152         8499 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYASV010000152 DAYASV010000000
VERSION     DAYASV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.157"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..1199
                     /locus_tag="ACGI68_003279"
     CDS             <1..1199
                     /locus_tag="ACGI68_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0139700.1"
                     /translation="YQTKELGKDFVIESCNSIKPWLINIRRELHKIPELALEENLTKQ
                     KVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEENNIPYKSIH
                     SGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKFLVEDGCLE
                     NPKVDYIFGLHVMPHIETGFIEIKYDTLNASVDTIKISIKGKRAHGAYPENGIDAIVT
                     ASQIVTSLQTIISRNLEPNNAAVLTIGKIYGGDAHNVICEDVKLEGTLRTLNSKTRNF
                     MIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELLGEEKFILR
                     PNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLPIGVMMHVM
                     NTLYFN"
     gene            1236..2585
                     /locus_tag="ACGI68_003280"
     CDS             1236..2585
                     /locus_tag="ACGI68_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0139701.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKNGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFLGNYGKFFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPMVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
     gene            2699..3261
                     /locus_tag="ACGI68_003281"
                     /pseudo
     CDS             2699..3261
                     /locus_tag="ACGI68_003281"
                     /inference="COORDINATES: protein motif:HMM:NF037635.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3369..5702)
                     /locus_tag="ACGI68_003282"
     CDS             complement(3369..5702)
                     /locus_tag="ACGI68_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139702.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIVIAVCLVTAIFGLADMAI
                     QCQKVQFIKDDGNWHIVLKNLNEEAISEISSRVDIAHYGRGQVIDNGEFKSKEILIQG
                     LDEDLAKQLGLEIIKGRYPVVENETLIDVDAVKSFNLKVGDFIKIKLADGRNKNYKIV
                     GEYSSLSRLRASDKHGLMLSILGTQAIKKSETYDYYYITFKDKVNIRKATSDIKNTFN
                     LSDKQIGKNEKLLSIIGQGDSDYSKPLYLTAGGLFILVLIASIIMIYNSFNMGVIERI
                     KFFGLLRCLGASKLQVKKFVVLESLILSVKAIPLGLLLGCIVTIISSIFLKYVNTELF
                     GSMPILKVSFIGIVFGIIVGFLTVILSAIVPAKKASGVSPLSAIRGNLMFKEILTSGK
                     ASKIANAGSSKIDVSMGINHAFSRKKSFILMTSSFAISIVLFLGFSVFIDFMYQALKP
                     IKPEAAHISIVDADFKSVLTKDDINEISNIKGIKKVYSRMMTDVEATYNNKEATSQLI
                     SYEKNQFDWAKKYLVKGEINEDKLESENIGLVEEGHGFNIGDKIYIKNGNSKKEIEVA
                     GILSSISFDVKDKYIGNIITSENTFTSITDIKDYIIIDTQVDEDAPEDLIQNIRNVLD
                     DNLEVRDKRQGNLEAKNSFYTMAVFVYGFVFIIAIISMFNIINSMNISVTSRINYYGI
                     MRAIGMSNKQLRKMVIVESSTYAVSGCLLGSVLGLILHRYIFVSLVTLKFHIDWQIPF
                     DLLFIIVVTMIIITLLAVRKPIKKICELDIIEVVNAQ"
     gene            complement(5699..6382)
                     /locus_tag="ACGI68_003283"
     CDS             complement(5699..6382)
                     /locus_tag="ACGI68_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139703.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGKIFVDGKEICSMKEEKQTIFRRRNIGFIFQSYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKSHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSSDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGM
                     LKDLGVNRE"
     gene            complement(6453..7694)
                     /locus_tag="ACGI68_003284"
     CDS             complement(6453..7694)
                     /locus_tag="ACGI68_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139704.1"
                     /translation="MKVFSNKDIKIFFLVIISVLLMFIVVGQIVVINITNDYKSALLE
                     HDYNIAGYLNSMGVDKSKIPAVFTTNEDKYTLKGKEILNTARYDFGTDDSFMPSVKLF
                     QGKYIKTMFLYLFVFFTMIIFILYVYFFRQQNKIEEASYKIDSFMNGNINVRLNTYEE
                     GSLSKLFGSIDAMSTSLNTHILKEKQNKEFLKNTISDISHQLKTPLTALMMYNQILQE
                     ESHNSEIVENFVQKSQNELERIESLIQNLLKITKIDSKTIILNKASVNVKKLITKILY
                     SFETRAEKEGKSISIRGLENTNLFCDYEWISEALSNLIKNALDNTKENDKIMIEWVET
                     PITTIISVSDTGNGIHIEDIHHIFKRFYRSKFSKSNQGLGLGLPLVKSIIEQHNGTIT
                     VESNFLQGSTFTLSFLKLTNM"
     gene            complement(7705..8391)
                     /locus_tag="ACGI68_003285"
     CDS             complement(7705..8391)
                     /locus_tag="ACGI68_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139705.1"
                     /translation="MSSILLVEDDLSLIYGLEYSIQKNGFSVDVARTVEEALQIYKEK
                     NYDLLLLDVSLPDGDGFDICKRVREASNVPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNFTSENVTELKSNNITVKLLENRVFKNEIELELTTAEYKLL
                     CLLMKNKNIVLTRKNILDKLWDGNGSFVDDNTLSVYVRRLRNKIEDNPENPKNLLTVR
                     RMGYKWNVIK"
BASE COUNT         2947 a         1316 c         1089 g         3147 t
ORIGIN      
        1 aatatcaaac caaagaatta ggaaaagatt ttgtaataga aagttgtaat tctataaaac
       61 catggcttat aaacataaga agagaattac ataaaattcc agaattagct cttgaagaaa
      121 atcttactaa acagaaggta atttcttatt taaaagaaat cggaattgat tatatggaat
      181 ttacaaaaca caatggaatt atggcatata ttttaaaaga aagtgcagat aaaacgattt
      241 gtataagagc agatatggat gctcttccta ttgaagaaga aaacaacata ccttataaat
      301 caatacattc tggaaaaatg catgcttgtg gccatgatgc acatacaacc atgctacttg
      361 gagcatgtaa agttttacat tcaataaaag ataaacttaa tgtaaatgta aaattcttat
      421 ttcaaccagc agaagaaggt tttggaggag caaaattctt agttgaagat ggatgtttag
      481 aaaatcctaa ggtcgattat atattcggac ttcatgttat gccacatatc gaaacaggat
      541 ttatcgaaat aaaatatgat acattaaatg ctagtgtaga cactataaaa ataagtatca
      601 aaggtaaaag agctcatggt gcatatcctg aaaatggtat tgatgcaata gttacagctt
      661 cacaaattgt tacatcatta caaactataa taagtagaaa cttagaacca aataatgctg
      721 ctgttttgac tataggtaaa atatatggtg gtgatgcaca caatgtaata tgtgaagatg
      781 taaaactaga aggcacttta agaaccttaa atagcaaaac aagaaacttt atgattgata
      841 aaatagctaa aattgttgga gacactgcat ctgcatttgg ttgtgttggg actttacatg
      901 taagtgatga aaactatcct gctgttataa atgaaaaaga attagtagat acagttatat
      961 caaatactaa agaattactt ggagaggaaa aatttatatt gagaccaaac ccgtctttag
     1021 gtggtgaaga cttttctttt tacacagaac attgtaaagg agcattcttc catttaggat
     1081 gtaaaaatga ggaaaagggc ttaatttcac ctcttcatac ctctagcttt aatatagatg
     1141 aagattgttt accaataggt gttatgatgc atgtaatgaa tactttgtat tttaattagt
     1201 acagtaatta ataaaagaat aggagataaa ttaatatgga aaaagctaag aaattaggtc
     1261 tatttagtat gatattatta gggattaata gcataatagg ttcaggtata ttcttactac
     1321 caggtaaagt ttataatcta gcaggtcaaa acagtatgtt tatatacatt tttgctacac
     1381 tcttagttct atcaatactt ttatgttttg cagaagttgg aagcatgttt gataagaatg
     1441 gaggagcata tctatactcc aaaaaagctt ttggagattt tataggattt gaagttggta
     1501 caatgtcttg ggttattaga attatatcat ggtctactct agcagtagga tttgcaacag
     1561 ctttaggttc attttggcca gaatctgcca ctgaatacaa aggttatata gctgcaatac
     1621 tcgtaacact actttcaata aatagcttat ttggaattaa aagcacaaag ataatgaata
     1681 atgtaataac tatagctaaa ttagtaccat taatagtttt tataatagtt ggaatattct
     1741 ttataaaatt tgtaaatatt gttccttcag gaaatgtagt taactctagt atgggtccag
     1801 ccattatatt agtattttac gcatttaccg gctttgaatc atttattgtg gcaagtggag
     1861 aaatggaaaa tcctaaaaaa aatcttccag tcgcattaat aaccacaata tttatatgtg
     1921 caataattta tatattaatt caaattgtct gtatgggtat acttggagat agactatttg
     1981 aaaacagtat tccaattgca gatgcttcaa gtgtattttt aggcaattat ggtaaatttt
     2041 tcatatctgt agcaacatta atatctatat ttggtataaa tataggttca tcaatagtaa
     2101 ctcctaaatg tggttcttct ctagcagaag aaggttctct tcctgcattt ataggaaaaa
     2161 caaataaata tggtgctcca tatgtagcaa taataatatc tttaatttgt tgtataccac
     2221 ttgtgctaac tggaagtttt gaacaacttg cagtaatgag tgtaattgca agatttgctc
     2281 agtacatacc aacttgttta tcagtaatag ttttaagaaa aagaacagat gttaaggcct
     2341 cttttaagat tccatttggt cctgttatac caatggtagc tattttaggt agcttatggt
     2401 tgttacaaca agcttgggca gaagatattt caaaacctat aactcaaaat agagttttaa
     2461 taggattagg ggcaatgttg ttaatagctc ctctatatat atttatgaaa aaaaataagg
     2521 aaatcgagca taaaaataaa tcagaagatt ctaaattata tatttcgaga gaaaaaattc
     2581 aataatattt tacaaactat ttaaaaatta tttttctaat actatagaaa taattagaaa
     2641 tggagatgtt acgtatctaa aagtagttga taacacggat attggtactt ttatagatat
     2701 gggacttaca aaagacatac tagttccttt taaagctaag acttatccat tgtttagaga
     2761 tgaaaaatat ttattctata tatacttgga taaaagcgga agaatagctg ctactacaga
     2821 catagattca tatttagaaa cagaccatac ttacaatgta ggagatgttg taaatggagt
     2881 tgtatatgga ttccaaacca acaactctgc tatgatatgt gttgacaata aatatgctgg
     2941 tgtaatcctt cataatgaat actttacaga attaaaagct ggggatgttc ttgaaaattt
     3001 acatgttata aaaatatacg atgatggtaa attaggactt tctcctagag gaaacagaaa
     3061 agatgaactt gataccttag aaaataagat tttaagctat ttagaaggtt cagatggata
     3121 tatgagattc aatgataaat ctgatcctaa agatatttct atcttattta attcaagtaa
     3181 gaaaatttta aacgtgctct tggagtactt atgaagaaag acttaattta tcaagatgaa
     3241 gatggaactt atctaaagta aattatataa cacaaaaatc gaagtaaatt atgaaagata
     3301 ttttctattt catatttact tcgatttttt atttaaatta ctacctctat ttaaattact
     3361 acttatattt attgtgcatt tactacctct attatgtcta attcacaaat ctttttaatt
     3421 ggttttctta ctgcaagcaa agttattatt atcatagtta ctacaatgat aaacagtaaa
     3481 tcaaaaggta tttgccagtc tatatggaat tttaatgtta ctaaggatac aaatatatat
     3541 ctatgtaata ttaatccaag tacactcccc aataaacagc cacttacagc atatgtactt
     3601 gattctacta ttaccatttt tcttaattgc ttattggaca tgcctattgc acgcataata
     3661 ccatagtaat ttattctact tgtaacgctt atattcatac tgtttatgat attaaacata
     3721 cttataatcg caattataaa tacaaaccca tatacaaaaa ctgccatagt gtaaaatgaa
     3781 tttttagcct ctaagttacc ttgtcgctta tctcgaacct ctaaattatc atctagaaca
     3841 tttcttatat tttggattaa atcttcagga gcatcttcat caacttgagt atcaattatt
     3901 atatagtctt ttatatctgt tatactagta aaagtatttt cagatgttat aatatttcca
     3961 atatatttgt ctttaacatc aaatgaaatt gaagaaagta tacctgctac ctctatctct
     4021 ttcttagaat ttccattttt aatatatatc ttatctccaa tattaaaccc atgaccttct
     4081 tctaccaatc cgatattttc tgactctaat ttatcttcat tgatttcccc ttttactaga
     4141 tactttttgg cccagtcaaa ttgatttttt tcataagaaa ttagctgaga tgttgcctcc
     4201 ttattattgt atgtagcctc tacatccgtc atcattctac tgtatacttt ttttattcct
     4261 ttgatattac ttatttcatt aatatcgtct ttagtaagaa cagacttaaa gtcagcgtca
     4321 acaatagata tatgagcagc ttctggtttt ataggtttaa gtgcctgata cataaagtct
     4381 ataaatacac taaaacctaa aaacaataca atactaatag caaatgaaga agtcataagt
     4441 ataaaagatt tttttcttga aaaggcatga tttattccca tagaaacatc aattttagaa
     4501 cttcctgcgt tagcaatttt acttgctttt ccactagtta aaatttcttt gaacattaga
     4561 ttaccacgta tagcacttag tggagacacc ccagaagctt tttttgctgg aacaatagct
     4621 gataatatta ctgttaaaaa tcctactatt attccaaaca caataccaat aaagctaacc
     4681 tttaaaattg gcatcgaacc aaataactct gtattcacat atttgagaaa tatagaggat
     4741 ataatggtta caatacatcc taataacaat cctagtggaa tagcctttac acttagaatt
     4801 aaactttcta aaacaacaaa ttttttgact tggagttttg atgctcccaa gcaacgtaat
     4861 agtccaaaaa actttattct ctctattaca cccatattaa agctattata tatcatgata
     4921 atacttgcta ttaacacaag tataaaaagt cctcctgctg ttaagtaaag tggtttagaa
     4981 taatcagagt caccttgacc tataatagaa agtaattttt catttttacc gatttgttta
     5041 tcacttaaat tgaaggtatt cttaatatca gaagtagctt ttctaatatt aaccttgtct
     5101 ttaaatgtaa tataataata atcatatgtt tctgattttt tgatagcttg tgtacctaat
     5161 attgaaagca ttaatccatg cttatctgag gctcttaatc ttgataatga tgaatattca
     5221 cctactatct tataattttt atttctacca tctgctaatt ttattttgat aaaatctcct
     5281 acttttagat taaagctttt aactgcatct acatctatta aagtctcatt ctccacaaca
     5341 ggataacgac cttttattat ttctagacct aattgttttg ctaaatcttc atctaaacct
     5401 tgtatcaaaa tctcctttga tttaaactct ccattatcaa tgacttgtcc tctaccatag
     5461 tgagctatat ctactctact acttatttct gatatagctt cttcattaag atttttaagt
     5521 actatgtgcc aatttccatc atcttttata aattgtactt tttgacattg tatagccatg
     5581 tctgctagtc caaaaatagc agtaactaaa catactgcaa tgacaataca taaaattgta
     5641 gttctatttt ttttcttatg tacctttgca tattgtggta ctagttctaa atagcttctc
     5701 attctctatt cacccctagg tctttaagca ttccatcaga aacctgtagt atcctatctg
     5761 ctgtagaagc tatatttgta ttatgagtta tcataataat agtttggtta tatttttctg
     5821 ctgtcacttt taaaagtgaa attacatcac tgctattttt actgtcaaga tttcctgttg
     5881 gctcatctgc caatataatt gatggctttg taatcaatgc tcttccaatt gcaacccttt
     5941 gttgctgacc accagataat tgatttggaa gatgactttt tctatcttta agacctaaaa
     6001 ttgtcaatac ttcatctaga taaatagtgt ctggtttttt gtaatctagt agtaaaggaa
     6061 atattatatt ttgttccaca gttaattcgg gtataagatt atatgattga aagataaaac
     6121 ctatattacg acgtcgaaaa atagtctgtt tttcttcttt cattgaacat atttctttac
     6181 catcaacaaa tatttttcct gaggttggag tatcaagacc tcctaaaaga tttaataatg
     6241 tactcttacc tgagccagat tctccaacta cagtaacaaa ctctcccttt gaaactgtaa
     6301 atgatacatc ttttaatgcc tctacttttg tatatccatt tccatatgtt ttacataaat
     6361 tattaaccac aagaatatcc atttgctcta tctcctttct gatgattaat ataagaataa
     6421 ttcatacacc ttactgtaag atgtatccta tcttacatat tagtaagctt taaaaaactt
     6481 agagtaaatg tacttccttg taaaaaatta ctttctacag ttatggttcc attgtgctgt
     6541 tctatgatag atttaactag tggcaaccct aatccaaggc cttgattact ttttgaaaac
     6601 ttactcctat aaaatctttt aaatatatga tgtatatctt caatatgaat tccatttccg
     6661 gtatcactca ctgaaataat agttgttata ggtgtttcaa cccattctat cattatttta
     6721 tcgttctctt ttgtattatc taatgcattt tttataaggt tgcttaaagc ttctgatatc
     6781 cattcataat cgcaaaacaa gtttgtattt tctagacctc ttatagagat actctttcct
     6841 tctttttctg ctctagtttc aaaactatat aaaattttag taatcaactt ttttacgtta
     6901 acactagctt tgttaagaat tattgtcttt gaatctatct ttgtaatctt taacaagttt
     6961 tgtatcaatg attctatacg ctctaattca ttttgactct tctgcacaaa attctcaaca
     7021 atttcactat tatgactctc ttcctgcaat atttgattat acatcatcaa tgctgtaagt
     7081 ggagttttta gttgatgtga tatatctgaa attgtatttt taagaaattc tttattctgc
     7141 ttttctttca atatatgtgt gtttagagat gttgacatag catctattga accaaataac
     7201 tttgataaac taccttcttc ataagtattt aaccgtacat ttatatttcc attcataaat
     7261 gaatcaattt tataactagc ttcttctatc ttgttctgtt gcctaaaaaa atacacatac
     7321 aatataaaaa taatcatagt aaagaacaca aataaataca aaaacatagt ttttatatat
     7381 ttaccttgaa acaatttcac acttggcata aaactatcat ctgtaccaaa gtcatatcta
     7441 gctgtattta agatttcttt tccttttaaa gtatatttat cttcattagt agtaaatact
     7501 gctggtattt ttgatttatc aacacccata ctatttaagt atccagctat attataatca
     7561 tgttctaaca gtgcactttt ataatcatta gttatattta taactactat ttgacctact
     7621 actataaaca tcaataaaac ggatataatc accaaaaaaa atatttttat gtctttatta
     7681 gaaaatacct tcatattaac accctcactt tataacattc catttgtaac ccattcttct
     7741 aactgttaat agattttttg gattttcagg attatcttca atcttatttc ttaaccttct
     7801 tacatatact gacagagtat tatcatctac aaaactacca tttccatccc ataacttatc
     7861 caatatattt tttcttgtaa gtacaatatt tttatttttc ataagtaagc ataataattt
     7921 atattctgct gtagtaagtt caagctcaat ttcattttta aaaacacgat tttcaagtaa
     7981 tttaacagta atattatttg attttagttc agttacattt tcacttgtaa aattataacg
     8041 tctaagtaaa gcttttattc ttgaaattaa ctcatttaat ttaaaaggtt ttgttatgta
     8101 atcatctcca cccatatcaa gtcccatcac tacatttact tcttcatcag aagcagttaa
     8161 aaatatgatt ggtacattag aagcttctct taccctttta caaatatcaa aaccatcacc
     8221 atcaggtaag gatacatcca aaagcagtaa atcataattt ttttctttat aaatttgcaa
     8281 tgcttcttct actgttcttg ctacatcaac actaaatcca ttcttttgta tagaatattc
     8341 taatccatat atcaaactta aatcatcttc aaccaacaat atactactca tctcaatcac
     8401 ttccattttt ttatttttta ctttatatat attatatacc taacttatta tatttttact
     8461 cttttaaaaa tacaatacct tgaaatcaat gatttaaat
//
LOCUS       DAYASV010000153         8375 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYASV010000153 DAYASV010000000
VERSION     DAYASV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.158"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(219..1895)
                     /gene="bclA2"
                     /locus_tag="ACGI68_003286"
     CDS             complement(219..1895)
                     /gene="bclA2"
                     /locus_tag="ACGI68_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HFH0139706.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGATGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGANGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(2273..2812)
                     /gene="hpt"
                     /locus_tag="ACGI68_003287"
     CDS             complement(2273..2812)
                     /gene="hpt"
                     /locus_tag="ACGI68_003287"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFH0139707.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGRVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(3140..4447)
                     /locus_tag="ACGI68_003288"
     CDS             complement(3140..4447)
                     /locus_tag="ACGI68_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HFH0139708.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEEIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(4872..6647)
                     /locus_tag="ACGI68_003289"
     CDS             complement(4872..6647)
                     /locus_tag="ACGI68_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0139709.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GDSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLERMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(6644..6781)
                     /locus_tag="ACGI68_003290"
     CDS             complement(6644..6781)
                     /locus_tag="ACGI68_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139710.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(6859..7428)
                     /locus_tag="ACGI68_003291"
     CDS             complement(6859..7428)
                     /locus_tag="ACGI68_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFH0139711.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(7612..8247)
                     /locus_tag="ACGI68_003292"
     CDS             complement(7612..8247)
                     /locus_tag="ACGI68_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0139712.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT         2393 a         1664 c          962 g         3356 t
ORIGIN      
        1 ttatcctaat ttattggtat atgaaatcat attgtttatt attttatagt cttttcatca
       61 tataaaccta ttaaatatta tcctatttat tggtatatga aatcatattg tttattattt
      121 tatagtcttt tcatcatata aacctattaa atattattct atttattggt atatgaaatc
      181 atattgttta ttattttata atcttttcat catataaact attgtattct ataaactgat
      241 acatatccag ctgcagaagt acctgtcgtg cctgaaccaa atgtaaaact taaagtaaca
      301 gctgctgctc tagcatcaac taaaaagctt aaacttacac ttacatctga tgaagtaagt
      361 gtatttactg cttttgcaat aaagttattt tgagctactc cattaaagtt agtagtaatt
      421 gacataaatc ctgcaagtga aagtgtggca cgtataccga aagatacatt gtatatccct
      481 ccaactgcaa gatttatagt actacttgtt ggaaaagtta tattagttcc atttataaat
      541 gatgaattaa atgtcactaa tgtattattc actaaacttg aagaggacac tgtaaattgt
      601 gcattgtttg ctgctaacac tcctgttgct ccggtcgctc cggttggacc tgttgctcca
      661 tttgctcctg ttgctccggt cgcccctgtt gctcctgtcg ctccatttgc tccagttggg
      721 cctgttggtc ctatcgctcc tgctactcca tttgctcccg ttgctcctac tgctccggtt
      781 gggcctgttg ctccatttgc ccctgttgct cctgtcgctc ctgttgctcc tgctactcca
      841 tttgctccgg ttggacctgt tggacctatt ggacctgcta ctccattcgc tccggttgct
      901 cctactgctc ccgttgggcc tgttgctcca tttgcccctg ttgctcctgt cgctccggtt
      961 ggacctactg ctccatctgc tcccgttggg cctgttgctc ctactgctcc tgttggacct
     1021 attgccccag ctactcctgt tgctcctgtc gctcctgttg gacctaccaa accatttgct
     1081 ccagttgctc cggttgggcc tgttgggcct accaaaccat ttgctccagt tgctccggtt
     1141 gggcctgttg ggcctgttgg acctaccaaa ccatctggac ctgttgctcc tactgctcct
     1201 gttggaccta cctctccatc tgctcctgtt gctccggttg ctcctattgg gcctgttgcc
     1261 ccagttgttc ctgttgctcc tgtcgctcct atacttccgg ttggacctgt tgctcctgta
     1321 tttcctgttg ggcctgtagc acctgttact ccatttgctc cggttgctcc tgtatttcct
     1381 gttggacctg ttattccatt tgctccggtt gctccggttg ctccagttag tccggttgga
     1441 cctgttgctc cagttgctcc tgtattccct gttggacctg ttattccatt tgctccagtc
     1501 gctcctgtat ttcctgttgg tcctgtagag cctgttattc catttgctcc agttgctcct
     1561 ttgtttcctg ttggacctgt tattccattt gctccggttg ctcctgtatt ccctgtcgga
     1621 cctgttattc cattcgctcc agtcgctcct gtatttcctg ttggtcctgt agagcctgtt
     1681 gttccatttg ctccagtcgc tcccatattt cctgttggtc ccgttattcc attcgccccg
     1741 gttgctcccg tatttcctgt tggacctgtt attccatttg ctccggtcgc tccagttgca
     1801 cctctaggcc ccgtcggtcc tgttggacca gtagcacctg ttggccctgt tggacctaca
     1861 tcttgatata aacttggacc tgaaatatca ctcatataat taatcctcct tttttaaagt
     1921 tagagtatta ctctataaat attgagtatt actctatata attatatgaa tcgacaattt
     1981 cttatgtgat aatgtatcta tttgcttata tcaacatata tgctttgatt cttgaatttc
     2041 attacttttt tgtaaaatct tttacaatta ttagtactct tttacaatta ttaatacaat
     2101 taaaaaactc attttccatc aattatttaa aattttttta ttatgttata aaaaatagat
     2161 aattaaattg cataaaatta atttaatact actttaatta ctttgtaaat aaaaaaacct
     2221 caacacgatt aataaaaatc atattaaggc atccacaaaa ttatataatt gcttatctat
     2281 cttcatcagt aacattgaat atataaggaa catttctata ataatcacca aaatcaaacc
     2341 catatccaac cacaaactta tcttctattt caaatccaca ataatctggc actaaatcga
     2401 cttttcttct gcttggttta tccaaaagaa cacaacattt taaactagca ggatttttag
     2461 cttttaaatg ttttaacaca aaatccatag taagtgcaga atcagtaata tcgtctacaa
     2521 ccaaaacatc atatcctgct atatcagtag tcacatctga aactacttta acccttccag
     2581 tactttcttc attgtttcca taactagatg tagtcataaa attaactcta agtggtaaat
     2641 ttatatttct aactaagtct gcacaaaata taaagctacc ttttaataga gaaactacca
     2701 taagattttt atctttataa tctttttcta taattgctcc taattccctt aatttatctt
     2761 taattttttc ttctgaatac aatacttccc atttttttgt ttctatatcc ataaaaagac
     2821 ctccaattgt aataatcctt cttaatttta taagagattg ctcacttttt caatactctt
     2881 attcaatatt agtcaatatt attgagtttt tatgttcatt caacaattat taacaatgta
     2941 tcttatattt taagtagttc atatatccac actaaattac atactatata ttttagacaa
     3001 aaaaagaaaa aacttgcact aacaagtttt ttctattata cataaattta tacataatca
     3061 tattttatta gagggtacta attaaattta tttatgctat ttttgttata cctcataata
     3121 caaaacatat tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta
     3181 tctcatctgt tatagacata cctttgtcac ttactctacc taagttttgt atactttgct
     3241 caacacttga accaactata ccatttagtg gtggtacaaa acaatcttgc ttagctatta
     3301 tagcactttg tatagctgca gatgctgcag aagataattt taatgcacaa ccagctttcg
     3361 caccgtcaca tatcattcca cttaagtcag ctatcatatt ttctatagct ccttctattt
     3421 gcttttcagt accattcata agccaagaga taccagcagt tgctccagta gaagcagcaa
     3481 caccacatcc acatacagca gataatcttc ctgtatagtt cttaatataa ccagttacta
     3541 gatgagatat agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattat
     3601 aagcaacgat tggaagaatt gctgttaatc catgatttcc acttccattt gaactcataa
     3661 caggcatttt tacccctgcc attcttgcat ctgaagctcc agcagttaac atcattgcat
     3721 aatttataac atcattacct aaaagacctt cttctataga ctttttaatt ccatacccga
     3781 caccaatacc agttttttgt tttaatccat attcagccat ctcttcattc atttttactc
     3841 catctagtaa aaattctata tctttaaaat caatttcttc tacatttttt attaattctt
     3901 gtatagttac tgtatccatt aaactttcag ctttttctgc cgcatcattt gaagctgata
     3961 cttttggttc attgtctaat aatacttctc catctttttc taagaacgta aaattatcat
     4021 gtttagttct tattctcact ttagcaatat gattttttcc ttttaacaca acttctataa
     4081 atactttctc tcttgtatca gcaggtgtta ttgatagtgg agtattatcc atataatcct
     4141 cagctatttt aacttcctct tttgttagag tttctaacac acttaatcca ttttcagaat
     4201 gtccacctac tatacctaga gctgctgcaa tttttaatcc taatctttca gtacctggta
     4261 tacctacaca cataccattt ttatagatac taggactaac taacatacga ttttcaacta
     4321 tttcttctcc taataattcc tttgcctttg cacatgctag agctaaggct actggttcag
     4381 tacaccctac tgctggttta acttctgcct ttaacatttc cactaattct tttcttatat
     4441 ctctcattat atataaacct cccaattaag tttagattta gtactcaaat aattagttaa
     4501 ataatttctt atctatattt atagcaaaat acatgccata ttttttatat ctttttatat
     4561 ttttagtctt atattctata accattggta tgactgcctc tatttatgta ttttttttat
     4621 ttgtatagag tatttatatt ctttaaaatg gagaaacata tgacaatttc gcaaaaagac
     4681 aaaaaaaagt ctcaaaatga gattctatat cattttgaga cggctcaaat atattatttt
     4741 ttaacttata aaaaccattt tttaattcat aaaaaaagat tctgcaaaac acttatagta
     4801 taagtttaaa gctttcttta aagttatgaa tttagaaaaa tttaaatttt atatagcttt
     4861 ttattatatt actatgaaat tatattgtat ttttcaattt ttctatataa tgtagctctt
     4921 gatattccaa gtgctttagc tgttaattct ttatcttttt tataatcttt atacttattt
     4981 aaagctttta ttatctcaat cttctctagt tcatcaagtg gaattatcct atcgctttta
     5041 acttctaact cttctacaga ggtattctta attttttgag gaattacatt cattgtgatt
     5101 attgaagaag aagacatatt cacactatat tcaataatat tctgcaactc tcttatattc
     5161 cctggccact tatattccat catagcatca acaaccctgc tatctatgtc aataacattt
     5221 ttatctaatt tttttgaata ctctttaacc atatgcttga ttataagtgg gatatcttct
     5281 tttctctctc ttaaacttgg taattttata ggtataacat ttaatctata atataaatct
     5341 tctctaaaag tgccattttg aaccattctt tctaaatctt tatttgtagc agcaataatt
     5401 cttacatcta ttgaaacatt tgatttactt ccaattttat taagttcctt ttcttgtaat
     5461 actctcaata actttgactg taaatgcaaa ctcatatctc ctatttcatc taaaaatata
     5521 cttcccttat gagctacttc aaacattcca agctttccac cttttttagc tccagtaaaa
     5581 gctccctctt catatccaaa taattcactc tctaataaat tatccggtat agcagcacag
     5641 tttacagcta taaaaggatt atctactcta ttgctatggt tgtgtatggc tcttgcaaat
     5701 agctccttac ccgttccact ttccccagta ataagtactg ttgatgttga tttagaagct
     5761 attagggctt cctttttaga atttcttata gctacacttt ctccaattat gttttccaat
     5821 tttattctat aatctttatt catcttattg tagtttttta tagcatctct tttatctata
     5881 aaatcaataa catatccctt taactcattt tttaatacta ttttattgat gttgtatatt
     5941 cccttcgaat catatccgta tcttttataa aaaaaactac aggatttgtc cttcttaaag
     6001 ttatttatag aaggtttctt tataaaatct aactcgacaa atatgttttt gccagttata
     6061 ctatctttta tattgaaaag ctctttaaat tttgaattat atttatcaat ttttccatcc
     6121 acatcaatag acacaacagc tttgtccata ttgtttaata gtgtctctaa ttttttcttt
     6181 tctaattcaa gctcataagt tttaatttgt gcttttaatt tattagatat taggtctgcc
     6241 attttgccta aaaagtttat aagaccactt ttattatttt ctataatttg tgcttgttct
     6301 tttgtaaagg ctataaggcc tataacacca tgagaatcgc catcacatat aataggacaa
     6361 cacacctctg caaactctac acaagcccca cttttatagc attccctaca tatatcacta
     6421 ctctttgggt catcaattag tattgtaatc tgttctgtaa gtgatttttt aaaagccgta
     6481 tatttatcta ctttctcacc tattttgtct atatatactc ctgtacctgc aatcctaaat
     6541 aactccttat ttactatagt tacatctata ttgagtactg atactatagc ttcagctata
     6601 ttttgaatat cagattttat atattctaaa cctatcataa tcatcattcc ttatttttct
     6661 ttagcataag tatttgtctt atttctttaa gaatcaagta tatcttattt aaagtaaatt
     6721 ggattgacat caggattaca aagagcataa caattattat taactcataa tttatttcca
     6781 tctaaacatc tccttataac aaaagactag gcttaaccta gtcttttata ttatatccat
     6841 atttatattt taactcattt atcttctttt tcccacataa atatatttta atttatttga
     6901 ctctttaatc ataccatcaa agtttaatct tgaaaatata ttaaatactt catgtggata
     6961 gtgaatcttt gcaacagaac cacaaaattt atgtttaaga tagtttaact taacttttaa
     7021 attttttaaa tttaaatcct ctaatataac ctctccattg ccctttaaca ctctttgtat
     7081 ttcagatgaa gctttttcac aatctgtaat aacattaaat tggtcaacta ttattatctt
     7141 gttgaactga ttatcatcaa atggtagaaa ctctaactta cctttaacta cattgcaatt
     7201 ttcattatac actagagaat aacagacatc ttcataatta ctattctcca gtatagttac
     7261 agaagttcca agacctctca atctcttgcc taggtttcct atatgaccaa caagcaatat
     7321 tttatcacct acattatact caattgtatc caaaaaacat tctaattcat cagatgtata
     7381 tttagcttta actggctgtt tatttatctg acataatatg cttttcacaa tgcttcacct
     7441 cttcatttcc tcaattataa attatgtaca gattacatta taataaatgg cgagcaacta
     7501 ttcaagagat atttcattag tattttttta aacaatcttt atacatttta atatgatatt
     7561 ataccaaatt tattaggatt ttattcataa aaatcagtct attttttatg tttattcatt
     7621 gttattttca tccaattttt ttattttcga tatggatact tcatatgcca tttttttgac
     7681 cacattattt tcatctattt ttttctcata ttctctactt tgaaccctac cccaaagctg
     7741 tattctatca ccaaccttta gattctttgc aaatttagca tttctacccc aaactattga
     7801 tggtatataa tcagatttat tataaggtct gtttatagca actaataaat cggttatttc
     7861 ccttcctaaa ggtgtcttcc tatacacagg ttccttacat acatatccat ctaaaaatat
     7921 actattaggg tctttatttt cctcgtctat actttttatt tctctgacaa acactgtaag
     7981 cactaatcta tttttattat ttaatgtttt gttatatgat cttaattggc ctacgacatt
     8041 gactaaattt tgtttatcaa ggtcaatatc ttggagaagt ctttctgata cagtcattgg
     8101 aagtatatca aaagaatcgc ttagtctgtt tatttttatt ttcatattat aaaatttttc
     8161 tccaaatatc tcatggctaa attctagctt attgtctaac tcccctctta agtttactac
     8221 gttattctca tctttaacag ctatcatatt ttatccctcc acaattgtta attcttatat
     8281 ttctggtatc agcttatata ataaatatat ttataaggga tgtattttat tacaaatctt
     8341 tgattgctgt ttaaaagttt ttatttagtt ataaa
//
LOCUS       DAYASV010000154         8299 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYASV010000154 DAYASV010000000
VERSION     DAYASV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.159"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..575
                     /locus_tag="ACGI68_003293"
     CDS             <1..575
                     /locus_tag="ACGI68_003293"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HFH0139713.1"
                     /translation="LKKLVKILINIICIITLIFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNIDYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEAFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNSNYEFKDYLNYIT"
     gene            complement(1588..2250)
                     /locus_tag="ACGI68_003294"
     CDS             complement(1588..2250)
                     /locus_tag="ACGI68_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139714.1"
                     /translation="MKMLKVIDIKKIYGKGENEIKAVDGITLEIEPHKFSAIIGQSGS
                     GKSTLLHCMAGLDKPTLGNVFMDNLDLYTLSDEKLSNLRSKEFGFIFQNYNLIPVINV
                     YDNIVLPILIAKGKIDNNYINDLITKLGLESQINKFPNELSGGQQQRVAIARSLANKP
                     SIIFADEPTGNLDSKTTNEVMNLLKYCVNEYKQTLVMITHNDEIAKMADCIITISDGK
                     VI"
     gene            complement(2267..4822)
                     /locus_tag="ACGI68_003295"
     CDS             complement(2267..4822)
                     /locus_tag="ACGI68_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0139715.1"
                     /translation="MGIYLKMSLAYLKKNKLRTLLLIIGVMLGVMLIFGPSVIKDSQN
                     ENNVVAIHELYSGYHVEFSDLTLDDAKKLETDEKVSKGNSVQNLGRISDKKGNSFILT
                     SSNEEYLKSRYNKIIEGRLPKNNNEIVLEKKALEAMNLSEGVGSTLNLTIKKNYKDTN
                     GSNQLFSINKEFKIVGVMEKPKGYYDVVWQYEAVTYGNDETNNIIPHDAISYNSILSF
                     KSGWRNIEGECDYLMKKYKLGQKSFLPNVPLVRELRYVEDDKNDPNVFKRNVLITTVS
                     IIFIFNIFNITLNQSVKEMGLLRLMGAKKRNIRCMIIYQALIITIIGILLGLVAGVTY
                     SYIGISNYNEVLYSEAGLNPKLYINNEIVIKAILVGMISVFISCIIPTFKIGKISPIE
                     SIINTDKIKNHRSRLGIDRLVSKIFGFYGFMGLKNIGRNKVRAAISMLSIALGGYIFI
                     TTFSSMQEEVNSKIEDMQNRYNITMQFGVNADPQISKYTDSDVENIEKIKGVKSIHKI
                     QITNGMFTFNNKDINKEFTTYNGVEKKEIMEDKLVLKLYDDDYIDEKLNDFVEEGSLD
                     DIKKDTDGYPNVAVYNYFYDIVDDHTIKPIFKELNIGDIITVKIPVEENNTIVYKEMK
                     VRVAVILKDEWIAMGDGDFMPDLEIITSNIHSKNITGEQKYTQLGINLENPYDEIVNN
                     EIQKISRSIPGSLFNSRLSYHEMSESIYKSYIKSQIAIIALVLIIASINIFCTIRTNL
                     LIRKKEISTLRALGVSLKNMKKMLIYEALAYAVFSFIIAMIPSFINLVKFTNWNNDAY
                     INYGIENFMSFTFPIKESLLFFAISVFVCLISVVVSNRDFESMNIIEGIKENE"
     gene            complement(5062..5250)
                     /locus_tag="ACGI68_003296"
     CDS             complement(5062..5250)
                     /locus_tag="ACGI68_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HFH0139716.1"
                     /translation="MRKYKAILLTLIISIIAIIGFIIYNNYFRKWNEAEKYIDKYMTY
                     QGVSGDDIERITKEKYTK"
     gene            complement(5262..5441)
                     /locus_tag="ACGI68_003297"
     CDS             complement(5262..5441)
                     /locus_tag="ACGI68_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139717.1"
                     /translation="MIHIEISEKEFFRKIEEVFDKGEVNVEVDNLGDIKIQVKKFDDK
                     TKSLELKLIKEFKLK"
     gene            complement(5446..5703)
                     /locus_tag="ACGI68_003298"
     CDS             complement(5446..5703)
                     /locus_tag="ACGI68_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139718.1"
                     /translation="MSKKISESIENSGLRVAENKSDGSFVLSLGNLEYTKEQPSQVLH
                     YSITKEKKDTKEILSIQCVKDFSYNEKLSEDDKFIMAIYNI"
     gene            complement(5676..5816)
                     /locus_tag="ACGI68_003299"
     CDS             complement(5676..5816)
                     /locus_tag="ACGI68_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139719.1"
                     /translation="MVIAISLTLGLITGCTNKVSGTNEKIYTIEQISSIKKTCQRKYQ
                     NQ"
     gene            complement(6083..6289)
                     /locus_tag="ACGI68_003300"
     CDS             complement(6083..6289)
                     /locus_tag="ACGI68_003300"
                     /inference="COORDINATES: protein motif:HMM:NF025854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0139720.1"
                     /translation="MCKYLDNAIEACDKIHDKSIDKYIQIKGTISKSYFVIKCENSKI
                     NSIRKNKSLLLTDKKDKLVHGIGI"
     gene            complement(6465..6710)
                     /locus_tag="ACGI68_003301"
     CDS             complement(6465..6710)
                     /locus_tag="ACGI68_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0139721.1"
                     /translation="MIPRLILASSLVIIIVFIKIIKENKLKLQNEVIKNKLDMQYSHY
                     LSIQESHAKVKKLYHDINNHIYCIENLKYKKGKLMGM"
     gene            complement(7350..7673)
                     /locus_tag="ACGI68_003302"
     CDS             complement(7350..7673)
                     /locus_tag="ACGI68_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0139722.1"
                     /translation="MKKIKINLTHTKSNLAKKYIEVQKKNMLIHTKNKTLDAICILEK
                     IEKDLNIEELVRCHKSYITNLNYVENIKSNTAILDSQEEVPISRYRYKDIKEGFLKFI
                     GDRIC"
     gene            complement(7912..8064)
                     /locus_tag="ACGI68_003303"
     CDS             complement(7912..8064)
                     /locus_tag="ACGI68_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139723.1"
                     /translation="MKNLNKNTILGEVIITITACDDEKEIQIVIKNQVDNILKNKNKE
                     YRIKKI"
BASE COUNT         2781 a         1201 c          855 g         3462 t
ORIGIN      
        1 aattgaaaaa actagtaaaa atattaataa atattatttg cattattacg ctaattttct
       61 ctagcctttc gatttacata aaattatctg aatataaaaa agctgatgaa gtatatacgg
      121 aactcagaga aaacactatc aataatagca aacatcaaga actttatgat aaaaataatg
      181 attatagatt ctggcttaaa ataaacaacg taaatataga ctatcctgta gtacaaggat
      241 ataacaatga tttctaccta actcatgatt tttataaaaa ttatcttcct ttaggtagta
      301 ttttcatgga ttatagaaat aattttgaaa atgataagag tttaatagtc tatggtcatt
      361 atatgaaaaa taaaactatg tttggtcagc tagaaaacta tactgatgaa gcatttttca
      421 aagaaaataa cttagtagaa ataaattata aagtacaaac ttatacttat gaaatttttt
      481 cagtatatac tgctgactta attaacagag attatttaag tatacacttt aatagtaatt
      541 atgagtttaa agattatttg aattatataa cctaaaaatc agtacacaaa tctgatataa
      601 aggtagatat ttcagataaa attataactt tgtatacttg taactatgaa tttaaagatg
      661 ctcgtactat agttcatgct aaattaattt caaaaaaaga gactgataag taggtttgtg
      721 taaatgcaga cttacctaca gtcttttttg aacgtaaaat tagtaagtat ggaataatat
      781 tcatattaaa cttttgtagg caaagaaatg aaaaatcaaa gagaaataat tattacagat
      841 ttagattacc aagctgaatt aagaaagtgt aaaactatgg aggatgtagt tggtgaagat
      901 agtatataat agtttaactt ataaattatc ttggtgatat taatgaagtt tcaaagtata
      961 tattatttat tgatataaat aatattaaat ctgttgctaa cagatgtacc tttgtttgga
     1021 gacaaactat ttaagaaata taactaaaat gttgaagtag ctacctgact ttattttgaa
     1081 atgcgaacaa gattttggca ttaagataat ttagaaagaa tcactggtac acaaggatgt
     1141 gaacttagaa tgaatagaag tattcaggca taaggagctt tcacccaaat aaaacatagt
     1201 atgaacttta aaagaattct aatcagaggc aatcaaaatg ttctttcata gtttatacct
     1261 gtagtgttag acatagtatt tataagtttc acaaataaga taatcattaa taaatgtagt
     1321 atgtatattc atcctctcaa aaatattttt taatcttaca ctttgtaaat tattctattt
     1381 taaaatggta tttttatacc tttatttctt atgaccgttt tcaagctcaa attttgcata
     1441 aatataaatt aatgaaaagc aagtaaaaaa agaactgtcg cacagcaatt taaaattact
     1501 cattcgacag tccttatttc gtggatttaa atagtggaga agaatcacaa gaataatacc
     1561 cacgattaaa cctttataca atacatttta tatgacttta ccatcactaa tagttattat
     1621 acaatcagcc atcttagcta tttcatcatt atgagttatc ataaccaacg tctgcttgta
     1681 ttcatttaca caatacttta gtaaattcat cacctcatta gttgttttac tgtctaaatt
     1741 tccagtaggt tcatctgcaa aaattattga tggcttattg gcaagtgatc ttgctattgc
     1801 aactctttgt tgttgaccac ctgataattc attaggaaat ttattaatct gactttctaa
     1861 tcctaactta gttattaaat cattaatata attattatct atctttcctt tagcaattaa
     1921 aataggaaga actatattat cataaacatt gataacaggt attagattat agttttggaa
     1981 tataaaccca aattctttac ttctaagatt agatagcttt tcatcactta aagtatataa
     2041 atctaaatta tccataaata cattacctaa agttggttta tcaagccctg ccatacagtg
     2101 aagtaatgtg ctttttccag aaccactttg tccaattatt gcactaaatt tatggggttc
     2161 tatctctaaa gtaattccat caacagcctt tatttcattt tctccttttc catatatttt
     2221 tttaatatct ataactttta acatcttcat acatttacct cccaaattat tcattctctt
     2281 ttataccttc aataatattc atactttcaa aatctctatt acttacaact actgatatta
     2341 aacatacaaa tacagatata gcaaagaata gtaaagattc cttaattggg aatgtaaaag
     2401 acataaaatt ctctattcca tagtttatat acgcatcatt attccaattt gtaaatttaa
     2461 ctagatttat aaatgatgga atcatagcta ttataaagct aaaaactgca tatgctaatg
     2521 cttcatatat aagcattttt ttcatatttt taagactaac acctaatgct ctaagcgttg
     2581 aaatttcttt ttttctaatt agtagatttg ttcttatagt acagaaaata ttaattgatg
     2641 ctattattaa aactagtgca attattgcta tttgtgattt aatgtaactt ttatatatac
     2701 tttctgacat ttcatgataa cttaatctac tattaaatag agaccctggt atacttcttg
     2761 aaattttctg tatctcatta ttaacaatct catcatatgg attttctaaa tttatgccta
     2821 attgagtgta tttttgttct cctgttatgt ttttagagtg aatattactt gttataattt
     2881 ctagatctgg cataaaatcg ccatctccca tagcaatcca ttcatccttt aaaattacag
     2941 ccactcttac cttcatctct ttataaacaa ttgtattatt ttcttcaaca ggaattttta
     3001 cagttattat gtctcctata tttaattctt tgaatatagg ctttatagta tgatcatcaa
     3061 ctatatcata aaaataatta tatactgcta catttggata tccatcagtg tcctttttaa
     3121 tatcatctaa gctaccttct tcaacaaagt catttaactt ttcatctata taatcatcat
     3181 cataaagctt taaaactaat ttatcttcca ttatctcttt tttttctact ccattatagg
     3241 ttgtaaattc tttgtttata tccttattat taaaagtaaa cattccattt gttatttgaa
     3301 ttttatgtat acttttaaca cccttaattt tttctatatt ctctacatca ctatctgtgt
     3361 attttgatat ttgcgggtct gcatttactc caaattgcat tgttatatta tatctatttt
     3421 gcatatcttc aattttactg ttgacctctt cttgcatact tgagaatgta gtaatgaata
     3481 tatatcctcc taaagcaata gacaacatag atatagcagc tctaacctta tttcttccga
     3541 tatttttaag tcccataaat ccataaaatc caaatatttt gcttactagt ctgtctattc
     3601 caagtctact tctgtgattt ttaattttgt ctgtatttat tattgattca ataggtgata
     3661 tcttgccgat tttaaaagtt ggtataatac aagatataaa aacagaaatc attccaacca
     3721 atatagcttt tataactatt tcattattta tatataattt agggttaaga ccagcttcac
     3781 tgtataaaac ttcgttataa ttactaattc ctatatatga atatgtaacc ccagctacta
     3841 acccaagtag tatacctatt attgttataa ttaatgcttg atagattatc atacacctta
     3901 tgtttctttt ctttgcgccc atcagtctaa gcagtcccat ttcttttact gattgattta
     3961 gtgtaatatt gaaaatatta aatatgaaaa ttatagatac tgtagttatt aaaacatttc
     4021 ttttaaaaac attcggatca ttcttatcat cttctacata acgaagttcc cttacaagag
     4081 gtacatttgg tagaaaactt ttttgtccca acttatattt tttcataaga taatcacatt
     4141 caccttcaat atttctccat ccacttttaa aacttaatat actattataa ctaatcgcat
     4201 catgaggtat aatgttattt gtttcatcat tgccataagt tactgcttcg tattgccaaa
     4261 ccacatcata gtaccctttt ggtttttcca taactcctac tattttaaat tctttattta
     4321 tagaaaataa ttgattactt ccgttagtat ctttatagtt cttttttatt gttaaattta
     4381 atgtagatcc tactccttca cttaaattca ttgcttctag tgcttttttc tctaatacta
     4441 tttcattgtt gtttttaggt agtctacctt ctattatttt gttgtatcta ctttttagat
     4501 actcttcgtt tgatgaagtt aatataaatg agtttccttt tttatctgaa atacgtccta
     4561 gattttgtac cgaattaccc tttgatactt tttcatcagt ttctaatttt ttagcatcat
     4621 caagtgtcaa atcactaaac tctacatggt atccactgta aagttcatgt attgctacta
     4681 cattgttttc attttgtgaa tcttttataa cactaggtcc aaatatcaac ataacaccta
     4741 acataactcc tattattaaa agtaatgttc taagtttatt tttctttaaa tatgctagtg
     4801 acatttttaa gtaaatcccc atataatcct cctattttac tataaattat ttatccaact
     4861 catctgttaa gtagattggt ggatatttta aatcttttaa ttcttcatta ttttttgaag
     4921 cttactacca tcttcctcat gataaacttg cagtatcacc ttatttacat gtagtgcata
     4981 ataatcacca ttataaaaat attagtattt ataacctgga taatttttgt aaaatgattt
     5041 ataaagtatc ccatcctaat tctattttgt atacttttct tttgtaatac gctctatatc
     5101 atcacctgaa actccttgat atgtcatata tttatctata tatttttcag cttcattcca
     5161 ttttctaaaa tagttgttgt atataataaa tcctattatg gctataatgc ttataattaa
     5221 agttaataaa atagctttgt attttctcat agtgaatctt cctacttcaa tttaaattct
     5281 ttaataagtt ttaattctaa agacttagtt ttatcatcaa atttcttaac ttgaattttt
     5341 atatctccca aattatcaac ctctacattt acttcacctt tatcaaatac ttcttcaatt
     5401 tttctaaaaa attctttctc acttatttct atatgaatca atgatctata tgttataaat
     5461 ggccattatg aatttatcat cttctgacag cttttcatta taagaaaaat ctttaacaca
     5521 ttgaattgat aatatttcct tcgtatcctt cttttcctta gttattgaat aatgtaaaac
     5581 ttggctaggt tgttcttttg tatactccaa gtttcccaaa gacaaaacaa aacttccatc
     5641 tgatttattt tcagctaccc taagaccact attttctatt gattctgata ttttctttga
     5701 caagtcttct tgatactaga aatctgttca atagtatata ttttttcatt tgtacctgaa
     5761 accttatttg tgcatcctgt tattaaacct agtgttaagc ttatagctat aaccatagtt
     5821 attgtaattt ttttatcttc atataatcct ccttatatat tgaaaactcg ttatttctca
     5881 taaaaaaaag acgctatctt tttctattaa aagataacat ccctatatgt ttagtatcaa
     5941 tatatgttcc ccaactggta ggttaatcga cccaactatc aaaattttga tttaatggta
     6001 tataaatatt caatataaat ttgtcttttt tatcttcaaa tgataaatct cctctatatt
     6061 tttttataga agatttgatg ctttaaatcc ctattccatg tacaagttta tcttttttat
     6121 ctgttaataa taaactttta ttctttctaa ttgaatttat tttactattc tcacatttaa
     6181 ttacgaaata ggactttgag atagttcctt ttatttgtat atatttatca atacttttat
     6241 catgtatctt atcgcaagct tctattgcat tatctaagta tttgcaaata tattagatac
     6301 atctattggt tttataaaat tcactttgga aaaattgata tcacaaatga atttaatttt
     6361 tcttttttca cttacatcac ttttttcatt tattattata tccaatataa cattacctgt
     6421 attgtaaatt gatttaaatt ctttaattta atcttttaaa ctatttacat acccatcaat
     6481 tttccttttt tatatttgag attttctata caatatatat gattatttat atcatgataa
     6541 agcttcttaa ctttagcgtg tgattcttga atacttagat aatgagaata ctgcatatct
     6601 aacttatttt ttattacttc attttgcaat tttaacttat tttccttaat tatttttata
     6661 aacactataa taatgactaa gctactagct agtattaacc taggtatcac aatataccca
     6721 actatatata tttcctaata cccaccaaat cgatacattt ttctatttat ataaattatt
     6781 aatatagtaa ataaatttgt tattaaagca acagataagt atatatagtt tattttatta
     6841 tctttatcta gtcccaatct tttattaata aatttaaata tagcacaagc tactatataa
     6901 ctattattat aagataatag tatatatcta ctttagcatt atcataacta aacactaata
     6961 gagcttcctc tgatgatatt acattacttg taactacaaa aaataaattt atagatataa
     7021 catcttttat taaagggtga tatacgtcaa ataagaaaaa tgaaactata tatttaacaa
     7081 ttcctatctt ataaaaataa ttatagtaag ttcccaagta ataaatatca aataatagat
     7141 tatatcatat agatttttct tcatattata atcatgggtc ttatcaacat taaataatat
     7201 aatgatctta gttataagat aagctaataa tacagtaaaa aaagatattt tcttagtctt
     7261 atctgtatct ttatcgtaag ttaatatact ttttagtatc attattggtg taaacaatga
     7321 taatattaat aatattttat ttatatggtt taacatattc tatcaccaat aaattttaga
     7381 aacccctctt ttatatcttt atatctatat ctactaatag gtacttcttc ttgactgtct
     7441 aatattgcag tatttgactt tatattttct acatagttta agttagttat ataactttta
     7501 tgacatctta caagttcctc tatatttaaa tctttttcta ttttttctag gatacatata
     7561 gcatcaagag ttttattttt tgtatgtatt agcatgttct ttttttgaac ttctatatat
     7621 ttttttgcta gattagattt tgtatgtgtt agatttattt ttattttttt caattttttt
     7681 ggtacatgtt aatatgtgtt tttctagttc ttgaaaattt attggcttta ataagtatca
     7741 ataagctctt acttcatatc tttcctgtac ataatttatt agagatgtta taaatattat
     7801 tatctacttt tcttatgttt cttgcgatat ctatactatt tagttctgcc atttttatat
     7861 ctaagagtag tgtatcctct caattaactt tgtaatgatt cttcttttga gctatatttt
     7921 tttaattcga tattctttgt ttttattttt taatatatta tcaacttgat tttttattac
     7981 gatttgtatt tctttttcat catcacaagc tgttattgta atgataacct ctcctaatat
     8041 tgtattttta tttagattct tcactttagg ttaaaacttt ttaaatgtat ttatcaataa
     8101 tatttaaatt aaaaatgtat aaaaatacct atatccaatt ggatataggt attcgtggat
     8161 ttcaatggtg gagacgaggg gagtctataa gtggttattt gattgttttg ttgttttttg
     8221 caagtgtagg tatatagcta tttagttatg atgttaaact gattttttcg gatatttttt
     8281 attgtttatt tttgtagtt
//
LOCUS       DAYASV010000155         8299 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYASV010000155 DAYASV010000000
VERSION     DAYASV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.160"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            279..989
                     /locus_tag="ACGI68_003304"
     CDS             279..989
                     /locus_tag="ACGI68_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0139724.1"
                     /translation="MINIGICDDELHYRIKIKDILSEILSSYPINYNIYEFSAGEELL
                     NNYPKDLDILIMDIQMKTINGMDTARKIREFDHKLEIIFVTSFVEFMQEGYEVKAYRY
                     ILKPINKEKISKSVLPCINEMMKKRNNYLTINVKNYVDRIKIDSITYIETDRPNILIY
                     THDDMYITKMSISKIEKILNEYGFFRCHNSYIVNLKLVESMSGSTVIVDGKSIPISKY
                     RVKGLKLAITNILGDIVC"
     gene            983..2299
                     /locus_tag="ACGI68_003305"
     CDS             983..2299
                     /locus_tag="ACGI68_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0139725.1"
                     /translation="MLTNLSVGFWNIINLVSGTIDWIIFFLVIHLVGKRKISNIRYIF
                     TTIIIIIFMAYINVVKVFPNIKILLCMVIGISFYITSYEDKIYKSIIVSLLFWLGLMV
                     AEGSAVGLVVFINKLNNINITQHENLFRIQAIIMSKVFLFIGFILFKYFKLSLDFKPK
                     DMILIGIPILSNIVSLLLIFEYNLRVNTITTENLFIFIFAILLILSSSIILLIVIGKI
                     VQDDKLNLEYELINERIKTNYRNYENINEVHDRLKYVYHDLKNHMICIKNYDTKEEII
                     SYINNLEFQISEFEHFKNTGNKTLDLILAEKISICKKYDIQIEDNINISKLNFIKNND
                     ICSIFANSLDNAIEACMDINNEIEKRIEVKATYINKFAIIKFINTKVNDIKLIDKRIQ
                     TSKDDDKIHGIGLASIKYIVNKYGGETIVNYSDNEFILKIMIPIKS"
     gene            2311..2451
                     /locus_tag="ACGI68_003306"
     CDS             2311..2451
                     /locus_tag="ACGI68_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HFH0139726.1"
                     /translation="MKKIALNLLKNISALSFGIAVLSANSASSWVAHQAKEPQALQKL
                     KK"
     gene            2510..3091
                     /locus_tag="ACGI68_003307"
     CDS             2510..3091
                     /locus_tag="ACGI68_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator ArgB-like protein"
                     /protein_id="HFH0139727.1"
                     /translation="MFKRLSYKFANILVNNEIVESEDFEIYRYGFETLIYFIVNILVA
                     LFIGIIFDRFIHTVIFLSCYCTLRQFTGGYHARNYKECTLTFAVIYLITIFSANNIDI
                     NKYKYLLVLLMIISILTIYKLAPLEHRNKPLSESEKKHYRKTVQKILFVIICLIILCK
                     ILNIFQQYVIYAIISIYWIAILIYIGMKVNSDQ"
     gene            3238..4947
                     /locus_tag="ACGI68_003308"
     CDS             3238..4947
                     /locus_tag="ACGI68_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139728.1"
                     /translation="MSSIKAISDFITKIIRKNILFAITIIIINYHLIQRWTHISNRGI
                     SFNDYLSLSVYTCMTISILTMLNVIFYMRKSYDTVKILCRNNFDYFLGISVAVTRNNF
                     IFCCIPIIYAIAYSSLIRTLSIVTIKSTIFILIFEWFLPLTILSIVISFISVIINNLI
                     LKITLSSVFLYLTSTNMLDRVIDIKEPFFKALFKSLNIFEDQSYAYYSDFVGKVNNSA
                     YVIDKIIPTLFAITLIIVAYLILNDIRKKQIKSLIVIVVYSILFIGLNYISIDMSTYR
                     DEERSYGEYLKKSNISDIKIKSHDMKIDFKSKSNIKDRIVLLNTSNEELTNINMILDE
                     IFDIKYIKINSENIKFNKKKDLIEVNLKESLEPNDEVTLDIEYEGYINVLTSWNQNKY
                     IATKDDIMLPPGSLAWYPKVNQSSCMAYKINLSSKNNVYSNLPLLNKTSNFLKKDYIF
                     EGKSNDIAIYAGEFKEEVKDDIKIIYPSDSNVNMDIEEKIKFLIETRNEILGDSKASV
                     DAKKYVDEVDNVMESIKRKKINMILVADLPSNEFLYEDSKGEKISITIYHWLSGNILI
                     IDK"
     gene            4962..5834
                     /locus_tag="ACGI68_003309"
     CDS             4962..5834
                     /locus_tag="ACGI68_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0139729.1"
                     /translation="MINKLEIRNLNFNYKNKKALDNINLTLIDGVVALLGPNGAGKST
                     LMRLLVTLYETSIGEIELNNIKYSKNNEKIKANVGYVPQDFDMYNNINGQEYLEFVAK
                     MRGVSKNDLKKHIQKVVSKVNLDKFINKKIGTYSGGVKRRLGIAQALIGDSKLIVMDE
                     PTVGLDPEQRNEFRRLLPEISKNSIVLISTHIVEDIQFNCDKLIILNQGKILYDGTIN
                     KFIDMVEVYSVVVSNDEFEYLERNIDIIDYRRVKDGVQVKYIGKNTEKLKDSLRIDTT
                     LQDAYINFLHKELK"
     gene            5835..6623
                     /locus_tag="ACGI68_003310"
     CDS             5835..6623
                     /locus_tag="ACGI68_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139730.1"
                     /translation="MCILELIKFELKKIELKHVLIFVIISILCIFITGILDYNELIFY
                     NDVPIMDTYSIAGWLGIIKISDVFIPIMSAITIIYVFSKDYSGNINEILTLYNKKKYN
                     QFIVFRWCVILAIYTVLLLISITIVYTFTSVKGFNIDKIDFVYSVKPIDVFLKTFPTL
                     LWYTTFPLMILTLTKNRFTTIAILITYTFADIFFILHMYPFVSMINVNGFYIQKMFYM
                     PNNIDVRFNELKNYFFINRGSLVVISIIIVSYIARKSTVLKKSK"
     gene            complement(6719..7552)
                     /locus_tag="ACGI68_003311"
     CDS             complement(6719..7552)
                     /locus_tag="ACGI68_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HFH0139731.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNCA
                     KKDNRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(7620..8249)
                     /locus_tag="ACGI68_003312"
     CDS             complement(7620..8249)
                     /locus_tag="ACGI68_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HFH0139732.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         3358 a          809 c         1166 g         2966 t
ORIGIN      
        1 aggaaggtgc atacttttta tttaacaaaa actagatatt aaaaactttt cgaggaatat
       61 tttattaaaa tccctaaata tatttaatat atattaaaaa attgtattat aatgaaaatc
      121 aaaatagatt gcaaatccaa aaagtaatac ttaatgtcca aaaagctaaa attaaaatct
      181 aaatagctat ttactacaga agtatgttaa aatatgtaaa atgaatagaa atttatatat
      241 ttttattaaa aatatataag aaaggatgtt taagaattgt gattaatata ggaatatgtg
      301 atgatgaact acattatcga ataaaaataa aagatatatt aagtgaaata ttaagttcat
      361 atcctataaa ttataacata tatgaatttt cagcaggaga agagttacta aataactatc
      421 ctaaagattt agacatatta attatggaca tacaaatgaa aacaataaat ggtatggata
      481 ctgctaggaa gataagagag tttgatcata agttagaaat aatatttgtt acttcatttg
      541 tagagtttat gcaagaaggt tatgaggtaa aagcatacag atatatacta aagccaataa
      601 ataaagaaaa aatttctaaa agtgtattac cttgtataaa tgagatgatg aaaaagagaa
      661 ataattactt aactataaat gtaaaaaatt atgtagaccg aattaaaata gattctatta
      721 cttatataga aacagataga ccgaatatat tgatttatac acatgatgat atgtatatta
      781 ctaaaatgag tatatctaaa atagaaaaga tattaaatga atatggattt tttagatgtc
      841 ataatagcta tatagttaac ttaaaattag tagaatcaat gagtggaagt actgttatag
      901 tagatggaaa aagtattcca ataagtaaat atagggttaa aggcttgaag cttgcgataa
      961 ctaatatatt aggggacata gtatgctaac aaatttaagt gtaggatttt ggaatattat
     1021 taatttagtt tcaggtacta tagattggat tatatttttt ttagtaatcc acttagttgg
     1081 aaagaggaaa atatctaata taagatatat atttacaaca ataattataa ttatatttat
     1141 ggcatacata aatgtagtta aagtgtttcc aaatattaag attcttctat gtatggtaat
     1201 aggaatatca ttttatataa catcatatga agataaaatc tataaaagta taatagttag
     1261 tttattgttt tggctaggat taatggttgc tgaaggttct gcagtaggtc tagtagtatt
     1321 tataaataaa ttaaataata ttaacataac acaacatgaa aacttattta gaatacaagc
     1381 tattattatg tcaaaggtat ttttatttat aggttttata ttgtttaagt attttaaatt
     1441 atctttagat tttaaaccta aagatatgat actaatagga atacctatat tatctaatat
     1501 agttagctta ttacttatat ttgaatataa tttaagagtc aatacaatta ctacagaaaa
     1561 cttatttata tttatatttg caattttatt gattttatca tcaagtataa tacttttaat
     1621 agtaataggg aaaattgtac aagacgataa attaaatcta gagtatgaac ttataaatga
     1681 aagaataaaa acaaactaca ggaattatga aaatataaac gaagttcatg atagattaaa
     1741 atatgtttat catgacttga aaaaccatat gatttgtata aaaaattatg acactaaaga
     1801 agaaattata tcatatataa ataacttaga gtttcagata agtgaatttg aacattttaa
     1861 aaatactgga aataaaacat tagatttaat tttagcagaa aaaataagta tttgtaaaaa
     1921 gtatgatatt caaattgaag ataacataaa tatatctaaa ttaaacttta ttaaaaataa
     1981 tgatatatgt tcaatatttg caaattcact agataatgca attgaagcat gtatggatat
     2041 aaataatgag atagaaaaaa gaatagaagt taaagctaca tatataaata aatttgcaat
     2101 aataaaattt ataaatacta aggtaaatga tattaaactt attgataaac gtattcaaac
     2161 tagtaaggat gatgataaga tacatggaat aggacttgct agtattaaat atatagtaaa
     2221 taaatatggt ggtgaaacaa tagttaatta ctcagataat gagtttatat taaaaataat
     2281 gataccaata aaaagttagg agagtacaaa atgaaaaaaa tagcgttaaa cttacttaaa
     2341 aacatctcag cattaagttt tgggatagct gtattatcag caaacagtgc aagttcttgg
     2401 gtagcacatc aggcaaaaga gccacaagct ttacaaaaac taaaaaaata acattaataa
     2461 aaggacctct tcttattaaa ggagaggttt tacatgttga ggaggaaata tgtttaaaag
     2521 attatcatat aaatttgcca atatattagt taataatgag attgtagaaa gtgaagattt
     2581 tgaaatatat agatatggat ttgaaacttt gatttatttc atagttaata ttttggtagc
     2641 attatttata ggaattattt ttgatagatt tatacataca gttatatttt taagttgtta
     2701 ttgcacttta agacagttca caggaggtta tcatgctagg aattataaag agtgtacatt
     2761 aacatttgca gttatttatt tgataactat tttttcagct aataatatag atataaataa
     2821 atataagtat ttattagtat tattaatgat tattagtata ctaacaattt acaagttagc
     2881 tcctttagaa catagaaaca aacctcttag tgaaagtgag aaaaagcatt atagaaagac
     2941 tgtgcaaaaa atactattcg taataatatg tttaattatt ttatgtaaaa tacttaatat
     3001 ttttcagcaa tatgtgattt atgcaataat ttctatatat tggattgcta tattaatata
     3061 tattggaatg aaggttaata gtgatcagta agattataaa acattttcta aattaattaa
     3121 cttcactaat taatattaac tttaaaaaaa taatgtcaag gtaagtattt gaacttatta
     3181 aatatagttg aatctattgt aggttgtatc aaaagactgg ctaggagggg cataaatatg
     3241 agtagtatta aggctatatc agactttata accaaaataa ttagaaaaaa tattttattt
     3301 gcaataacaa ttattattat aaattaccat ctcatacaac gatggacaca tatatccaat
     3361 agaggaattt catttaatga ttacttatcc ttgagtgtat atacttgtat gacaattagt
     3421 attttaacca tgttaaatgt aattttttat atgagaaaaa gttatgatac tgtaaaaata
     3481 ttatgtagaa ataattttga ttatttttta ggtatttctg ttgctgtaac tagaaacaac
     3541 tttatatttt gttgcatacc aattatatat gctatagcat actcaagttt aataagaaca
     3601 ttgtcaattg taacgattaa atcaactata tttatattga tatttgaatg gtttttacca
     3661 cttactattt tatctatagt gatatcattt atttcagtta ttataaataa tttaatacta
     3721 aagattacct tatcatcagt atttttatat ttaacatcaa ctaatatgtt ggatagagtt
     3781 attgatatta aagaaccatt ttttaaagca ttatttaaat cacttaacat atttgaagat
     3841 caatcatatg cttactattc tgattttgta gggaaagtta ataattcagc atatgttata
     3901 gataagatta tacctacact atttgcaatt actttaataa tagtagcata tctgatatta
     3961 aatgatatta gaaagaaaca gattaaatca ttaatagtta tagtagtgta cagtatttta
     4021 tttattggat taaattatat atcaatagat atgtcaacat atagagatga ggagcgtagt
     4081 tatggagagt atttaaagaa atctaatatt agtgatatta aaataaaatc gcatgatatg
     4141 aagattgact ttaaaagcaa gtcaaatata aaagatagaa ttgttttatt aaacacatca
     4201 aatgaagagt taacaaatat taatatgatt ttagatgaaa tatttgatat aaaatatata
     4261 aaaataaatt cggaaaatat aaaatttaac aaaaaaaagg atttaattga agttaattta
     4321 aaggaaagtt tagagcctaa tgacgaggtg acattagata tagagtatga aggctatatt
     4381 aatgtgttga catcttggaa tcaaaataaa tatatagcta ctaaagatga tattatgctt
     4441 ccaccaggtt ctttagcgtg gtacccaaaa gtaaatcaat caagttgtat ggcttataaa
     4501 ataaatttaa gttctaaaaa taatgtctat tcaaatttac ctttactgaa taagactagt
     4561 aattttttaa aaaaagatta tatatttgaa ggaaagtcaa atgatattgc aatatatgca
     4621 ggtgagttta aagaagaggt aaaagatgat ataaaaataa tctatccatc agatagtaat
     4681 gtaaatatgg atatagagga aaaaattaaa tttttaattg aaacaagaaa tgaaatatta
     4741 ggtgatagca aagcatctgt agatgctaaa aaatatgtag atgaagtaga taatgttatg
     4801 gaatcaataa aaagaaaaaa aattaatatg atattagttg ctgacttacc tagcaatgaa
     4861 tttttatatg aagactcaaa gggagagaaa attagcataa ctatttatca ttggctttca
     4921 ggaaatatcc ttataataga taaataataa aggaggttaa tatgataaac aagttagaaa
     4981 taagaaatct aaattttaat tacaaaaata aaaaagcttt ggataatata aatttaaccc
     5041 taatagatgg agtagttgca ttattaggtc ctaatggtgc tggaaaatca acattaatga
     5101 ggttattagt gactctttat gaaacttcaa ttggagagat agaattaaat aatataaagt
     5161 atagcaaaaa caatgagaaa atcaaggcaa atgtaggata tgtacctcaa gattttgata
     5221 tgtataataa tataaatggg caagaatact tagaatttgt agctaaaatg agaggtgtat
     5281 ctaagaatga tttaaagaaa catatacaaa aagtagtatc aaaagttaat ctggacaagt
     5341 ttataaataa aaaaataggt acatactcag gtggtgtaaa gagacgacta ggtatagcac
     5401 aggctttaat aggtgattct aagttaatag ttatggacga acctacagtt ggtcttgatc
     5461 cagagcaaag gaatgaattt agaagattgt taccagaaat aagtaaaaat tctatagtat
     5521 taatttcaac tcacatagta gaagacatac aatttaactg tgacaaatta attatattaa
     5581 atcaaggcaa aatattatat gatggtacaa ttaataagtt tattgatatg gttgaggtgt
     5641 attcggtagt agtttctaat gatgaatttg aatatttaga acgtaatatt gatattattg
     5701 attataggag agttaaagat ggtgtgcaag taaagtatat aggtaaaaat acagagaaac
     5761 ttaaagatag cttaaggata gatacaactt tacaagatgc ttatataaac tttttacata
     5821 aggagttaaa ataaatgtgt atattggagt taataaaatt tgagcttaaa aaaatagagc
     5881 ttaaacatgt gttaatattt gtaattattt ctatcttatg catatttatt acaggcatat
     5941 tagattataa tgagttaata ttttataatg atgtacctat aatggataca tattctatag
     6001 caggatggtt aggaattata aagatatcag atgtttttat accaataatg tcagcaatca
     6061 caataattta tgtgttttca aaagattatt cgggtaatat aaatgagata cttactttat
     6121 ataataaaaa aaaatataat caatttatag tatttagatg gtgtgttata ttagctatat
     6181 atactgtatt attattaatt tcaattacaa tagtatatac atttacgtca gtgaaaggtt
     6241 ttaacataga caaaatagat tttgtttata gtgtaaagcc aatagatgtg tttttaaaga
     6301 catttcctac attattatgg tatacaacat ttccactaat gatattaaca ttaacaaaaa
     6361 atagatttac aacaatagca atattaatta catatacatt tgctgatata ttttttattt
     6421 tacatatgta cccatttgtt agtatgatta atgtaaatgg attttatatt caaaagatgt
     6481 tttatatgcc taacaatata gatgttagat ttaatgaatt aaagaattat ttctttataa
     6541 atagaggttc tctagtagtt atttctataa taatagtttc ttatatagca agaaaatcaa
     6601 cggttttaaa aaagagtaaa tagtttaata tattttttat aatgttatat ttatatagaa
     6661 tagtgacttt aaaaaattta aagccactat tttttaatat gttttatcta tataactatt
     6721 atttttctag gttttttaat atttcgtaat agtgtgttgt ggctatttca cttgactcgc
     6781 cttttttaca aaaagataat ttagatagtt ttggaactac acgttcaacg acaattttat
     6841 tacctatcat tttagattca agtatcttca atgcttctaa aaatctatta tccttttttg
     6901 cacaattata aaatgataaa acgtagacat aaaggaatag gttataatta cggaaaggat
     6961 attcaacttg cataaataag gttccaattc catagtgaca agggccgatt ggttttttaa
     7021 tagtccaatg gtcaagtaga aaatctacag ctttgtcaag ttttgattct ttatttagat
     7081 aattactaaa acggaatgca tttaatatat taagagttgg tagtggattt gagtattcag
     7141 tttctggacc tcgaccaaaa ctaaatttat tgcaacgcca cccaccatca gtatactgtg
     7201 tatctaaaaa atgttgaaac gttttttgca ttctaatgtc agatgcatat cccatatgac
     7261 agagtacgtt agcagcattt gcagtatgac aagggtaaat acctccattt ggatatagtt
     7321 tgaaacgtcc atcttctcgc catgtgctaa atatcaagtc agcacattct ttaagtaaag
     7381 tatcatcagg gttcacacct aattctaata gataaagtac actatcaaga gtagagaaag
     7441 gagaaccttt tattaaacgc ttatcaggtg tagtccaata gtctgcacca ttatcatacc
     7501 tgtgtgataa tattatttca atatctgata aagattgatt ttctacattc attttttact
     7561 ccatttcata ttatattcta ttaaatctat caaaaacttt attataatat tatttatatt
     7621 tataattgat ttattttatt taaaattgca tttatagctg tatcaggaac aactgctaaa
     7681 tcttctgcat tgaagtaata aactatatta gaatcaaata atatatttga atttggagga
     7741 aattcatcat ctccaaacca aagtataaat accatagatg tattacccat atatgtaaac
     7801 ctataagcta aatctcccat gtttactttt tcagctccaa ttttctccat aactaactta
     7861 tatttgtcta tttgagatgt aaaaacttct gaaagcctat atagagtcct gtttaaaaaa
     7921 tttggataat aaacattgcc tcctggtatt tctttaaaag ttacatattt attagttcta
     7981 ggaatacctt tagcattgat taaatatcta actatcaaga ttttaagctc aaagttattg
     8041 caaaggctac cattactatt ccaaacttct ccacttggat agtctataat atattgtcta
     8101 tcaaaaaaat tgagtgtgaa tctatttaaa tcattattga aaggtatatt acttaaatta
     8161 ctaattgtat gagggtctaa ttctttgagt ttttttcgaa catagtcaaa tggaacatta
     8221 taattatttt ttgttatctt ttcatccata attttttcct ccaatttaaa caattaaaag
     8281 tatatttata aaaagtttt
//
LOCUS       DAYASV010000156         8279 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYASV010000156 DAYASV010000000
VERSION     DAYASV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.161"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(129..1076)
                     /locus_tag="ACGI68_003313"
     CDS             complement(129..1076)
                     /locus_tag="ACGI68_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139733.1"
                     /translation="MRITKTTFNDVFSNDANGSIIKVEKYVDKKCASVFEILTYTIIV
                     TNTSRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGYIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
     gene            complement(1104..1595)
                     /locus_tag="ACGI68_003314"
     CDS             complement(1104..1595)
                     /locus_tag="ACGI68_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139734.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            complement(2038..2931)
                     /locus_tag="ACGI68_003315"
     CDS             complement(2038..2931)
                     /locus_tag="ACGI68_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFH0139735.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            complement(3156..5441)
                     /locus_tag="ACGI68_003316"
     CDS             complement(3156..5441)
                     /locus_tag="ACGI68_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFH0139736.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKKFEVRVTRFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHIKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLLYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGK
                     PIKIIVYGKKYILEKCIKIDK"
     gene            complement(5438..6118)
                     /gene="pgmB"
                     /locus_tag="ACGI68_003317"
     CDS             complement(5438..6118)
                     /gene="pgmB"
                     /locus_tag="ACGI68_003317"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFH0139737.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            complement(6342..8225)
                     /locus_tag="ACGI68_003318"
     CDS             complement(6342..8225)
                     /locus_tag="ACGI68_003318"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFH0139738.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         2648 a         1399 c          730 g         3502 t
ORIGIN      
        1 aatattatac tatacttatt atttataatt gtttaattaa tcatttatat atatgataaa
       61 tatatcaata tctattttcc tttaaccaaa tgctatttta ataagtttaa gttttagctt
      121 taaaatactt aataatacaa taaaagtgat gtatttacaa ataattcttc tggatttaat
      181 tcatttattg atgtatgttc aatttttata tttatatttt tcatatcttc ataaataatt
      241 ccaactggag aacaaatgaa acaagaaaat ccaaaagttt ttgttatttt ttttacctta
      301 ttatcaccaa aacttatttg ctttttacaa tcgtttatat tttcattgtt attttgtata
      361 tcatttttat aatacttatc ttttttaatg tcattagact tatttactgt tttaaaatta
      421 ctcctatttt tacaactata gtctatttca acttctatat acccaaaaac tatcaagtta
      481 cataatttca aattactacc tttgtttata agatttttca taggtttaac atcaaatatt
      541 ttactattta catttacttt taatatatct cttagatttt taggagcttt taaagtatta
      601 cttatattca attgtttgaa tacaatttta tttacattag tataaactct attactttct
      661 attgatattc ttgttgggaa tttatctaga ttacacttat aataatagta tatattagca
      721 ctattcttta taatcctatg tgcagaattt ggtaaaacta cactcttaaa agatattatt
      781 tttttacaac ttgcatctat tctccctatg taaaaacctt gctgtgggtc taatccacgt
      841 tttataatgc cattaacttt taccgtatta tttataaact ctatatattt agaaatataa
      901 tctttgaaaa atatatttcc agttttgtac ctacttgtat ttgtaacaat aatagtgtag
      961 gtcaaaatct caaatacaga tgcacatttt ttatcaacat atttttctac ttttataata
     1021 cttccatttg catcatttga aaatacatca ttaaaagtag ttttcgttat tctcattgca
     1081 tccacctttc tataatttaa agattactct atcttggaac tattttcata acttataatt
     1141 gcagtaacac taataaataa attatcatct aaaatagatg aagtaatatc ttgtataaga
     1201 tactttaaat taattttatc tttattttct atctttctag gcatttgtat aaatgtacta
     1261 aagggtattt ttaatttaaa tgtatttttt ttactagaat ttctatttct tttattgcct
     1321 gcataatcta atattgcatc aatatctata tcaccaacta aaatcaactt ctttcctgta
     1381 agttgttcat cttctaatga tatacattct atagtatcaa caagacttat attgttaata
     1441 ttcatattgt aaacatatgt ctctaatgaa tttattacag tttttttaga agctaattta
     1501 aaatttttat atgtattaaa ttgagataaa taaagagtct tttctagttt ttcacatctt
     1561 tcaaggttgt atattacctt attattttca tacataatcg catcaatctc cctgtatcta
     1621 tatattaata ctattcataa aaattatttc aaattaccat tttttattat tcactatcta
     1681 gttgttattt acttttaaga aagaatcctt ttttacttca ttgatttcaa tttacaagta
     1741 aaagtctttc tgaaaaaaaa atatatatat ctctattcac ttttttataa gtaattataa
     1801 tttttataga ctaacggtca gccttaaggg aaaaggtgtt ctttaagtgc agaatttaga
     1861 taaagtaaat aatcctttta taaataattc caagttttaa cttaaataat ttgatttaca
     1921 gtataaagta aattttgaaa gataatgata tcgatttaat aattacaagt aaaagcatgt
     1981 caaaataagg ctatgatttt aaaaatcata gccttatcaa taaaaattta atcaaagtta
     2041 catttcactt tgaatttcac tgtctattaa ttgaagatta tactgttgaa ttaattctat
     2101 tagacgagct gcatatatac tattgccatt ttcatccttc gcagtactat aacctgcctt
     2161 ttctaatgct ttagcttgat atatatacgt ttttgcatca aatacaccat tgtttttata
     2221 ccttttattt gtggctaaaa attttgcatg gtcttttata gattcatttt tatcatcata
     2281 tattctaaat ttatcatcta taattgtatc taaatgttct ttcgtttcta aagtcactga
     2341 cttgcctttc caataagcgt cagcctttat accaaataga ttgttatatt gttttgccaa
     2401 agttgacttg ccccagctag attctattat agcttgtgca atagttatag atggaagtat
     2461 tttataatcc ttataatttt gtattgcttc tgttttaatt tcatcaataa acttcatata
     2521 ttttgtatca gacttcaatc tttctggttt tataccataa tccttaagat tgtctatata
     2581 attatcaacc aatctttttt gtctattact aaattctaac tcatccaaaa tatcacttaa
     2641 cttatttatt ttattatttt tagaaaaatt cttaacaaat aaatctgata cttcttgtat
     2701 ctgtgaatcc ttaacgtttt ttaagttatt tttatttaac acagcaacaa tggaagctac
     2761 atacttccag ttaacttgtg cattattttt acttgctaaa tcagcatatt ttatatactt
     2821 actgacattt atatcatcct tttttatctc tctagatgaa tattccttca ctaatacacc
     2881 tattaaaatt gatattatca gtattataac aatgaatttt ttattcttca taaatattgt
     2941 cctcccttat actatctgct gaatataata taccttactt ttgtgacata ttctttacga
     3001 aattttgaaa attttatgaa gatttatgaa atatatttaa ttaaaagcac tactattgtt
     3061 ataatatata gttttctatc acacaaaata gttaaaataa ataaataatt agatagctta
     3121 ttttaactat ttatataata aaaatattat taaatctatt tatcaatttt tatacatttt
     3181 tctaatatat attttttccc atatacaata atttttattg gtttaccttt taaattttct
     3241 attttaatta aatcactaca tacatctaca ttaataatat tatctctata atttatattg
     3301 aaagaataat ttttccactc ttttggcaac ttagggtcaa aagatagagt ttcattccat
     3361 gttctcatgc ctgcaaatcc atgtacaatg gaaagccatg aaccagacat tgaagttatg
     3421 tgcagtccat catttgtatc attattgtaa ttatctaaat caagcctagc tgttcttgaa
     3481 tataattcat aagccttttc taggttatct atttctgagg caataataga ataaatacta
     3541 gaagataaag aagattcatg tacagtatat ttttcataga agtcaaaatt tctctttttt
     3601 tcttctttag taaatctatt tccaaaatag taaattccct gtaatacatc tgcttgtttt
     3661 ataaaacacg acctaagaat cttatcccaa gaccaatttt gattaagagg aaaattttct
     3721 tttggtaaat cctttacttc tacaaattct ttatctaaaa aattatcatt ttgaattatt
     3781 atttgtagtt cctcatcata cagcagatac attttatttg atattatttt ccaaaactca
     3841 acttcttccc tagtcacatt atttctgtca atacattcat tacactcttt ctctaatcgt
     3901 aagatatttt ctacagcata atctaaacac catttagcta tataattagt ataccaatta
     3961 ttattgatat tgttttcata ctcgtttgga cctgttactc catgtatcat atacaaatct
     4021 ttttttgtag acaaatgaac tctacttgcc caaaatctag ctatttctat tattacatct
     4081 attccttttt cttttatata tttatagtca cctgtataat ttgtatagtt atatattgca
     4141 tatactatac ttccatttcg gtggatttcc tcaaatgtta tctcccattc attatgacat
     4201 tctactccat caaatgttac cattggatat aaagcacctt taagacctag tttttttgca
     4261 ttatcctttg cctgttctaa ctgattgtac ctatattcta aaagatttct agttacctcc
     4321 ttatctgcta cacctagata tactggaaca caaaacgctt ctgtatccca ataagtagct
     4381 cctccatact tttcaccagt aaatcctttt ggacctatat ttaatcgcga atcttctcca
     4441 taatatgttg aaaatagttg gaataaatta tatcttatac cttgttggct acttttatct
     4501 ccatttattt ttacatctgc actttcccat cttttattcc ataactttat atgttcattt
     4561 aatatatcat cataactttt agatagttct ctatttaaaa tgtcttcaca ttttcttaat
     4621 agctcacttt ccttataatc tctagttgag gttatagata catatttttc tatacttgca
     4681 acctcatttt ttaaaacatc cattgttatc gaattttcac agtaattata atcactctca
     4741 tgagatacag cttctacatc accatttgtc ttaatataca tcattgaacc cacagtgaat
     4801 actggtgtat taaatgcatt ctcctttgtt cgagaaagaa tactcccttt atattctaaa
     4861 gaacttgaag aaattccatt ccaaaacttt tcctcatagt ttgaatcttc attttttata
     4921 tttgaatcta aatatggtac aaattttatt ttagtattag aatttaatga ttttatactg
     4981 tatttaatta ctgcaagttc cttaacagat atacttaaaa acctagtaac cctaacttca
     5041 aattttttat catttatatt tacaataaac ctacgattta aaataccatt tttcatatct
     5101 aattctctat gaaaatcttc tactttacat tttgctaaat ctaattctac atcatctatg
     5161 aaaattctta cacctatata gttgactgaa ttaggaattt taccaaagta ttctggatac
     5221 ccatttttcc accaaccaac acgagtttta tctggaaacc atactccagc tatataagac
     5281 cccttgtgtg tatctccact ataattttcc tcatagtttc ctctcatacc catatatcca
     5341 ttgcctatac tcattatgga ctctgctaat ctattttctt tgatttcaat cttatcttgt
     5401 ataatcttcc actcatctac attactaaat aaacatttca tataattctc ctaactatat
     5461 actcattata cttttcttgt atatattcaa actttaattg atttgttgaa tctacaacta
     5521 aatttgcttt ttttaagttt tcataatttc caacaccaac agaaaacata ttagctgagt
     5581 ttatagcgtc tatacctgca cttgcatcct ctatccctat gcaattttgt ggatttacat
     5641 taagcccttt cgctgacata agaaatatct ctggatgtgg tttattgttt ttacattttc
     5701 ctgcatcagc tataaaatca aatttgtcac ttatacccaa atgatttaat acatttattg
     5761 cattcttact tgcagaagat agacctatct ttatattatt agattttaca tcaattaaca
     5821 agctttctat gcctggcaaa atatcatttg aagttatctc atctatcaat gatacatagt
     5881 aattattttt ttcttcagcc attctcactt tttcttcttc tgaaaatgaa tatttcttat
     5941 ttccaaattc taatatcctg tccaatgact ccattctact tatgcctttt aaagattcat
     6001 taaattttgt gtctatatct atcccaactt tatgagctag ttttctccaa gccatatagt
     6061 gataatatgc agtatctgta attacaccat ctaaatcaaa tataaatgct tcaatcattc
     6121 ttatttcctc tttctacaag aaatttttag actaataatc aatttatttt ctatcttgta
     6181 tttaagatat tatttataaa attatctact ctaactcagt ctttaattta aaatctactt
     6241 tcaattcact cacttgtaga ccatattttt tatcgtttat aaagacttgt ttaaactgta
     6301 taataattta acatgaaatc taaaataaat acttttttgt attattttaa aataatgaac
     6361 ttgtatttct ctaacattag ttcttctttt aaatcaacta tttcttcttc atataaatct
     6421 tttacttttt tatttgctaa ataatctggt aacgtcaaaa ttatattttt agatgaatta
     6481 tttattatta tagaaataga ttcttttttc aatataatcg ttccattttc cttatctgct
     6541 aacacccatt catttgatat agacctaagt tctttatttt ctttacgaag tctaataatc
     6601 ttcttcataa aatcaaatat atccttattc catttttctt cattccattc catacatttt
     6661 ctttgacctt cagatattga acttaattct ccttccattc cgatttcatc accatagtat
     6721 atacaaggac atcctgtttg tacaaacata aacatatatg caagtttaaa tctatcaata
     6781 tttccattag cataagtcaa tattcttgta gtatcgtgac ttccaagtaa attaaaaata
     6841 acttcactaa cttgtattgg ataacttacc attacatcat ttatcatata cttaaattct
     6901 tcggcgttta tctcatctgt acaaaagaat tttttcatgg cttctgacat caagtaattc
     6961 ataactgaat caaattggtc acccattaac catggtaaac taccatgcca aatttctccc
     7021 aatatataaa tgtctggttt tacttttttg acttcttctc taaactttct ccaaaaaaca
     7081 tggtctactt catttgaaac atctaatctc cacgcatcta tatcaaattc ctgtatccag
     7141 tacttgccta catccaataa atattttatt acttctgaat tttctgtatt aagtttaggc
     7201 atatattttt cacacccaaa ggtttcataa ggtatatttt tttcatctat ttgttctatt
     7261 ggagtatcaa ttttactcat atcttttata taaaaccagt ttttatatcg agaattttcc
     7321 ttattttgaa ctacatcttg ccactgttta gaataatatc ctatatgatt aaaaacagca
     7381 tccaacatta tttttatatt tctcttatgc gcttcttcta ttagtttttt taaagtctct
     7441 ttatctccta atgtagggtc tacctccata taatctaccg tatcatatct atgattggtt
     7501 tttccaattg taatagggca aaagtaaatt ccgtttattc ccaaatcact caaataatct
     7561 aagtgttcta tcacaccttg taaatctcca cctgtaaaat tatctctggt tggaatagct
     7621 ccccatttat ctactccttc tgggtttata gacgggtcac catttgcaaa tctatctgga
     7681 aatatctgat accaaacagt atctttcacc caatttggca ctttaggtat atctatataa
     7741 tttaaatatg gatatccaaa aaaattacct atttcacata attccttttc atcattttca
     7801 tttcccaact ctattattct tttttctgta aataaaagtt tttcctcttg accttctatt
     7861 ataaagccat atcgtgagcg tttatttaaa ggcttacact tagctatcca atagtcaaat
     7921 aattcagttt ctacttcttt actcattact atatttgttc caccagacca accaactcca
     7981 gatgcattta aatttcctcc tcctgcacct cctttatccc acacatattg gtctcctatt
     8041 cgtaaaatta ctcttttcgc ttcatttttt ttagttctaa gtcttatatg tagtgtttct
     8101 ctatcataac cataggcata attgctcatt ggtatatgta atatagcttc ttttgttaaa
     8161 ctattttgaa ttgttccttt gatttgatta cttaaagtta tattttcttt atttttatta
     8221 gacattttaa aatttccctc ctttataata atttatataa tctaaagata ggttaatat
//
LOCUS       DAYASV010000157         8171 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYASV010000157 DAYASV010000000
VERSION     DAYASV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.162"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..597
                     /locus_tag="ACGI68_003319"
     CDS             <1..597
                     /locus_tag="ACGI68_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139739.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="ACGI68_003320"
     CDS             728..1750
                     /locus_tag="ACGI68_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD0873 family tyrosine ABC transporter
                     substrate-binding lipoprotein"
                     /protein_id="HFH0139740.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="ACGI68_003321"
     CDS             complement(1914..2471)
                     /locus_tag="ACGI68_003321"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0139741.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="ACGI68_003322"
     CDS             complement(2477..3553)
                     /locus_tag="ACGI68_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFH0139742.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="ACGI68_003323"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="ACGI68_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFH0139743.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="ACGI68_003324"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="ACGI68_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0139744.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="ACGI68_003325"
     CDS             5391..6320
                     /locus_tag="ACGI68_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0139745.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="ACGI68_003326"
     CDS             complement(6481..6930)
                     /locus_tag="ACGI68_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139746.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8005)
                     /locus_tag="ACGI68_003327"
     CDS             complement(7133..8005)
                     /locus_tag="ACGI68_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0139747.1"
                     /translation="MYNSSVGLEQLIKNEDIVKIFSERKDRPLEKLKNYKTSELMIQS
                     SNNYKLESLFITSNIKTRDTMILVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGN
                     SGGDNYTFGLYERYDLDSLVKFVKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVD
                     FYILDSPYSEMKDAIRMGVLEKRIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVE
                     KSNVPILFIHGTKDTVCNYQNSKKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNK
                     IFKFIDSNVLSDKS"
BASE COUNT         2824 a         1349 c          982 g         3016 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttctcattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta ttttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac tcctaatctt ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acgttataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtctatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt tgccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataattgat t
//
LOCUS       DAYASV010000158         8038 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYASV010000158 DAYASV010000000
VERSION     DAYASV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.163"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            95..535
                     /locus_tag="ACGI68_003328"
     CDS             95..535
                     /locus_tag="ACGI68_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139748.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            862..1089
                     /locus_tag="ACGI68_003329"
     CDS             862..1089
                     /locus_tag="ACGI68_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFH0139749.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            1115..3235
                     /gene="feoB"
                     /locus_tag="ACGI68_003330"
     CDS             1115..3235
                     /gene="feoB"
                     /locus_tag="ACGI68_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFH0139750.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKNSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(3368..4186)
                     /locus_tag="ACGI68_003331"
     CDS             complement(3368..4186)
                     /locus_tag="ACGI68_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0139751.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            4405..4677
                     /locus_tag="ACGI68_003332"
     CDS             4405..4677
                     /locus_tag="ACGI68_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139752.1"
                     /translation="MKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKAVKLE
                     IFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   4845..5071
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4912..4940
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(5730..7823)
                     /gene="pdcA"
                     /locus_tag="ACGI68_003333"
     CDS             complement(5730..7823)
                     /gene="pdcA"
                     /locus_tag="ACGI68_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdcA"
                     /protein_id="HFH0139753.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVKNPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SVQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         2847 a         1132 c          987 g         3072 t
ORIGIN      
        1 ttaataaata taaattttaa taaaggtatt tatgttaaaa atggtataat gtatcttaat
       61 attcagtttt ttaagatgct aggaggtaat tactatgttt aaaattaaac actttaatga
      121 tttgagttta gatgaatttt atgaaatagc aaaatcaaga tatgaggtat ttgcttgtga
      181 acagaagata ttttcattaa acgattatga tgatatagat aaaaactctt atcacatatt
      241 tttaaaagaa aatggattga tttgtgctta cgctagaata attcctaaag aatacagctc
      301 ttataatgat gtttccattg gtagagtttt agttctttct agtcatagaa gaaaaggatt
      361 agcaaaacaa atgatggatt gtgctataga ttttataaaa gttaacttac acgaaaataa
      421 tataacctta tcagcacaaa cttatataaa gaacctttac ttatcttgtg gttttaaaga
      481 aatctcagaa gtatacgacg aagcaggtat tgaacatatt aaaatgagat tatagtgttg
      541 acaagctaat attgttaata tataatttaa gtataaatta aatattgaat tgatatttag
      601 gtatttgaaa ataccttaca tagagtcaga aggcttttgc tttctgactt ttttattatc
      661 aaaattcaat aattatatgg attcaaagtg ttaattttta ttctatattc tactttcccc
      721 aaaagtaaga attataccca actttgtatg ataggcatct tgcctatctt ttttttgtaa
      781 aagtaacaag gtaataatat aatgcatagt ttaaaataag aagattagcc caggctaaat
      841 ttcaagcatg gaggtgctat tatgaaaaac cttaacgata ttcaagtgaa aaaaactgtc
      901 aaagttgaag atatattatc tagtggtaat ttaagagaaa gaatgttagc gcttggtctt
      961 actagaggag ctgttataga tgtagttaga aaaggtccaa aaaataattt aactgtctat
     1021 aaaatcagag gtagcaagat agctctacga caagaagaaa gtagcttaat attagtttct
     1081 gacatttaaa aatttctagc aggaggatat taatatgggg ttgacacata actcaactaa
     1141 aatgagttct ttaaaagaca tgtttgatat agacaataag gaagaccagt ttgtaattgc
     1201 attggctggc aatccaaata caggtaaaag tactgtattt aatcatctta caggacttag
     1261 acagcatact ggaaactggc caggaaaaac tgtagctaca gctcgtggta attttaaata
     1321 taaaaatacc gaatatgcat taatagattt accaggaaca tattctttat ttgccttatc
     1381 acaagaagaa attgtagctc gtgattttat atgttttggt aatccagatg cagtaatagt
     1441 agtatgtgat gctacatgtc ttgaaagaaa tttaaattta gtatttcaag tgatggaact
     1501 tacagataaa gttattttgt gtattaattt aatagatgaa gctagaaaaa aaggcattac
     1561 tattgataaa aaacttttag aggattctct tggtattcca gttattttaa ctgctgcaag
     1621 aaatggctct ggtatggatg aactattgga tactttaaat gatgtatctt ttgacaaata
     1681 taaattaaat aataaacctg ttagatacaa tgaaaatata gagaatgtgg taaaatcaat
     1741 tcaaccagaa ttggataata ttataccagg tataaattct agatggttgg gacttagact
     1801 tattgatggc gatgaaagta tatttgaatc tatgagtaat tatatagaca aaaattctat
     1861 agatgctatt aatgaagtaa agaaaaagat tccagacaat ataaataaac agaaaataag
     1921 agatgagttt actaaaatca actatgatta tgctaaaaaa ctgagtgatg agtgttgttc
     1981 taatgttgct aaaaaatcaa ctgatagaga agaaaaagta gataaaatat taacatctaa
     2041 aatatttggg cttccaataa tgctcttatt attaggtact atattgtgga ttactataga
     2101 aggagctaac tatccatcca ctttattatc taatttatta ctcggatttg agccaagtat
     2161 ttcgggtata ttaaatagta ttaattgtcc atcatggtta aatgatatgt tggttttagg
     2221 attatacaga acattagcat gggtaatctc tgtaatgcta cctcctatgg caatattttt
     2281 tccattattt accctacttg aggattttgg atatttgcca agagttgcat ttaacttaga
     2341 ccacctattt aaaaaagctt gtgcacatgg aaaacagtgt ttgactatgt gtatgggatt
     2401 tggatgtaat gcagctggag ttattggttg taggataatt gactctccaa gggaaagact
     2461 tatagctata cttacaaaca actttgttcc ttgtaatggt cgttttccta ctttgatagc
     2521 tatatctaca atatttttta gctcagttat aaccaactct ttcgtatcaa gcgtagcaac
     2581 tgcactttgc ataactttac tgataattct aggtgttata ataacattgt tggtttctta
     2641 cactctttct aagacgctat taaaaggagt tccgtctaca tttacattag aacttcctcc
     2701 ttatagagtt cctcaaatag gaagaacttt atatacctct ataatagata gaacaatatt
     2761 tgttttagga agggctgtaa tggtagccat acctgctggt gtcataactt ggatatttgc
     2821 aaatatatat ataggagatt taagtatatt atcacatgtt gcaaactttt tagacccttt
     2881 agctaaatta attggtcttg atggttttat attacttgct tttattcttg gattcccagc
     2941 aaatgagata gttgtaccta tacttttaat ggcttaccta gcaactggtt ctatgataga
     3001 attagatagc tttagtgctt taggtcaagt tcttagagaa catggatgga cttatttgac
     3061 agctttaaat gttatgctgt tcagtttact gcattggcct tgtgcaacaa cacttttaac
     3121 aataaaaaaa gaaactggta gtttaaaatg gactgcctta ggattcttaa tgcctactat
     3181 acttgctttt gttgtttgtt ttttaacaac tacagtttac aatttattta tataattagt
     3241 attaactgac agtagacata tattagagaa accatttaaa aatttatata ctttattata
     3301 aagcatagag atatattcaa tgataagtaa aattacttaa catttgaata tatctctatt
     3361 ttaattctta tctggatact tggattgaaa tttcagtcat ataattatct tcattacttt
     3421 cacaaaaaaa gtcaagtata ctctcctcat acgaatttcc aacaatttta tattcatttt
     3481 catatatata ttttttcaat ttttcgtaag atttatatga atcctcatat tttccaatat
     3541 gttttatgca agcatacatt ccttctggct ttacatatag ctttttatcc tttatttttt
     3601 tatctatctt caaataaaaa cttgatatac ttaaatagtc cccatttttt atattttcat
     3661 tacttactat tactcccaac tcatatcctt gatacaaatt tttcgattta caataattgg
     3721 taaaatctat taattccatc ataaaatcac tattactcat attactcaaa tcgcttgata
     3781 gaactaaata ttcttcaaac tttttggata tataaatttc ttcactattc attttagaaa
     3841 ttccttcaac tgttatattt attctatttt gcatcttaga acttatcttt cttagttttt
     3901 ttattttttc tcttatcttt ttttcttctt ctttaagcat tttcacacaa agctggggag
     3961 ttctattttg tatatactct tttatttcct ctaaactcat tcctatttct cttaatgatt
     4021 gtaatatatc aagtgtatca tattgataaa ctgagtaata tctataatta tttttatctt
     4081 tatattcagg agaaaaaata cctattttgt catagaaaat taatgtctgc ttagatatac
     4141 cacacaattt agcaaattca cctgttgtaa aatactgttt ttccaagggt tatacactcc
     4201 ttttcttttt gttgtctata tagttactat attctttatt atttactaaa tcaagtagtt
     4261 attattttag aaaatcaata tggttttgat tttaaagctt aaatatcagt tagatgtcag
     4321 ttataaatat tttacccttt cccatcataa aaaagatatc ccctcttaac ttttggatgg
     4381 aatattatat ttatacttgt tagagtgaaa attgtaatag gtgctacaat aagatattta
     4441 attaaacata aagttaatta ttgtaataaa aaatctttag ataaattaga gttaaagtac
     4501 ggaaatatag atagagaaaa agctgttaaa ctagaaatat tttatcagta tttaatagaa
     4561 cttgaatata tagaaatgag tttattaata agaagattag atactgcaat aacatcatta
     4621 gcattagtat ctattataac aatagcatca tattatttaa tcagaaaaaa tgtgtaatta
     4681 tgtaagctat ttataataaa acactgataa aattaataat aaaaaggtaa taggatttct
     4741 atactacctt tttgttttta taatatagtg taattttatt atatttttgt atgctaatgt
     4801 aactagataa tcataagata acacattaaa agaaaattat gaaaacttac attctgtata
     4861 gttaatataa aacataagta atataaattt atgtaaagca acaaaagata gatttacatt
     4921 ccatatagtt aatataaaac cttagagaaa gcgaaaacgt accattaaaa tcaaatattt
     4981 acattccata tagttaatat aaaacgttgt aatcatagtt ccaattagcc actttaacat
     5041 gcatttacat tccatatagt taatataaaa caccaaaata aacttagtat tttcaacact
     5101 tacacgtata caactctcct aaatttgcag tgaacgagca atagtgcaat tgataacgct
     5161 tatcatgcac tctcaacgcc ttatatttca actgttacgc caaatttttt cacaaatatc
     5221 gctcactgca aaattcctat aattttatta tatcataaat atattacttt tgaatattga
     5281 taccaattta taccataata aaagcaaaaa aacgtaatct atctacatta gatttaaatt
     5341 ctataactta aagtttttta cataatattt atgcttgttt aatcaatcta atatatcatt
     5401 cttgtatatt gcttttaata gtcttttttc ttaaataaat atctatctga acaagcatag
     5461 tttaattaaa aacttttgta ttcccttaaa actgtatcta cctcttatta ttttatttat
     5521 gatatattat ttttaatcta aaaatagata tctattttta ttatggttat ccctttttaa
     5581 aatactttaa tcatatacta ttcccaattt aacatccatt gaaatgctat tttcttatat
     5641 tgcaatttat aattgatata tcttcaatat aataatttaa tgtatttttg attttaaagc
     5701 ttttcagtga tattattgat aaattagaat taattatcta gctttaaaag gtcaaagatt
     5761 tctgttgctg tctgcggttt tccaaataaa tatccttgta tgtaatctgg ctttatttgt
     5821 ttaactaaat catactcttc ttgtgtttct acaccttcta aacatacttt tattcctaca
     5881 tcatgacaaa ttgcaacaat aaaatgtata aatgtagcat caaacttact ctttaatata
     5941 tctttaacaa atactctatc aattttcact atatctattg gagaaaattt taaaacttct
     6001 agagacgagt atccagtacc aaaatcatcc atagcaatat aaattcctaa atcttgtaaa
     6061 gccttaaatt taaactgtaa aatactcata ttttgtactg tatgactttc tgtcaattct
     6121 aaaacaagat tttgataagg aaaattttca tcatctataa tttttactat atcttcaata
     6181 aatgtgtctt ctaatatttg aacagctgaa acgtttacac tcactttaaa aaaaggatag
     6241 taatcaatcc atttcctaaa agtacgtaat gccattcgaa gcacccaagc ccctacaata
     6301 tttatcatat cattttcttc taatattgga ataaattcca aaggagaaat tgctttgcat
     6361 ttgtcattag tccaccttaa aagaacttct acacctatta ttttatgact ttctgtatct
     6421 acttgtggct ggaatcgaag actaaacccc ctaaaatcat cattaattga tgcctttaaa
     6481 tcccgcatca tttctaatga atacatttta ttttttaata tttcttggga gaaaaaaaca
     6541 agcctgtttt taccatgctc tttcgcatat tgtaaagagt aactagcaca cttataaagt
     6601 tcttttactg tatctccatg ttttggatat attacacaac cagcagaaat tgttatattt
     6661 aatccatatt ttctccatag ttgaaggtgt ataatcatat tctgtatttg attatataga
     6721 gttagaattt cattttcttc aacattatca acaagaatgc ccatttcgtc cccatcaagt
     6781 ttataaagtt cagcattatc tggtaaaata gattgtacag actgtgctag tgtttttatt
     6841 atattatctc caaaatccct atcatacatc tcattaacat ttttaaaatc gtcaatatct
     6901 aataatacta tgcataattt ttcaatcata ggatttgaaa ttttatcttc aaatacactc
     6961 ataaattcat gataattcag taactgtgtc aatgaatcaa tcttattttg tttacctaat
     7021 tgcgtcatta ttcctgcaaa aatacttggc tctccaaatt catcacgcat tagttgacct
     7081 ctacatctta accatatata ttctccacta cgatttttag ccctaaattc cactgtatga
     7141 taatccatct ggtttttacc tatttcagtg tttgacttat aaaaacgatt ccagtcttct
     7201 ggatgtacaa tttttttcca gtaaacaagc ggatttttaa caatttctcc tggtaagtca
     7261 aataattcaa cttgtgatgg tgaatatcga aatacgcctg ttttcatatt gcaaatgtat
     7321 atatattcat ctgtacctct aatcaaagca tcgtaaagca atgtagaatt ataatcaaaa
     7381 ttgtctcttg aaaaattaaa aactcctttt tcttctttat tatttgatat ttcatgaaat
     7441 tcattaatct taattggttt atttaagctt gttgagtttg caatatctat tgattgatgc
     7501 atatgaacaa ctgtgccatc ttgccatgta attaaactat cataattttc aaaaacacga
     7561 tttaacttat tacatttttc ataccacttt attaatactc cttttttatc attctttaat
     7621 aattctaaaa ctttacaaaa actacaagta gaattttttt caggatataa tacttgccaa
     7681 caaacttttc cttctggatc taaaatatta tattcttctt tcattttttt gttcataaaa
     7741 ataatttcat tagtatgaat atttgtaaca tatatattaa tctgtagttg atttaatata
     7801 acttcaaaat tatgtttgtt catatactta tctcctccat agttactgct ctttataaca
     7861 acattgttaa aattattctt tttagaagat ttacttataa cttatacata tacactaaat
     7921 accttaattt aattagaaag tttattactt catattttaa attataattg ataaaaaaaa
     7981 atacagcaat atagtattac caatttaaaa aaaattaata ttacataata catctctc
//
LOCUS       DAYASV010000159         7966 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYASV010000159 DAYASV010000000
VERSION     DAYASV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.164"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            190..1449
                     /locus_tag="ACGI68_003334"
     CDS             190..1449
                     /locus_tag="ACGI68_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFH0139754.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            1720..2703
                     /locus_tag="ACGI68_003335"
     CDS             1720..2703
                     /locus_tag="ACGI68_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFH0139755.1"
                     /translation="MTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIKVIKH
                     NNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGIGLDE
                     LILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDFGITK
                     ANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNSEEKM
                     LQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKAYIEV
                     EDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            2949..4016
                     /locus_tag="ACGI68_003336"
     CDS             2949..4016
                     /locus_tag="ACGI68_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFH0139756.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFAEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYSKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            4550..6460
                     /locus_tag="ACGI68_003337"
     CDS             4550..6460
                     /locus_tag="ACGI68_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFH0139757.1"
                     /translation="MNSNTFERLQLNEVKDLIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNVGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(6523..7146)
                     /gene="ytaF"
                     /locus_tag="ACGI68_003338"
     CDS             complement(6523..7146)
                     /gene="ytaF"
                     /locus_tag="ACGI68_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HFH0139758.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            7334..>7966
                     /locus_tag="ACGI68_003339"
     CDS             7334..>7966
                     /locus_tag="ACGI68_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139759.1"
                     /translation="MEQNNKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLK"
BASE COUNT         3369 a          789 c         1283 g         2525 t
ORIGIN      
        1 aagaaatagt aaaagaaata taataaaaaa tatcaaaaat aaaactaatt ataagacata
       61 aatttacaat aaagttacat ttgattaaat tccttgttta acaaaagcta ttcaaatata
      121 atctaaggta gttttgaaat gggaactcta ttatgcaaat ttaagctagt gttaaaatga
      181 ggtgaagtta tgggcagaag aacaacaaga gtttcaagat taggtaaaga aaaaaaacga
      241 agtgttttaa ttgtattggc agctttagtt ctaataatgg gtggatttac ttattttttt
      301 aacagtaaat ttctgtacaa tgggaaaatc gctaaaaatg tatatataga aggagtaaat
      361 gtatctgata tgactaaagc agaggcatta aaagctataa cagataagta tactccagaa
      421 gatttaaatc ttgcttatga tgggaaaaaa tatacaatat caccaaaaga tattgattta
      481 aaatatgata cagaagatgt tgtaaaagat gcttatgaaa gtacaaaaaa agggtcctat
      541 tttcaaaact taaaaaaata tattgatata agagtgaata aggcaaatat gaagataaaa
      601 gctgaatatg atgaggcaaa acttagttcc aaagtgtctt ctatagcaga tagtataaat
      661 gttaagatga aaaatgcttc tattagtgtt ggaagtggtg gactaagtta tacagactct
      721 gttgtaggta gagaatttga tttggcagca aataaagagt ctatatataa tatgataaaa
      781 aataaagagc ataaaacttt agaacttaaa gtaaatcttc aaaaaccaga tataacaact
      841 gaacaagtaa aaactgtaaa ttctgttata ggtcaatatt caactactta ttcacaagct
      901 gtagagggta ggtcatataa tgtaggatta tcagcaagaa aaactagtga tgtattatta
      961 atgccaggtg aagaattttc atacaataaa cttacaggac ctagtaataa agcaaatggt
     1021 tacaaagatg cacctgttat agtttatggt aagctagaac aatcagcagg aggaggtgtt
     1081 tgtcaaacat cttcaacagt ttataatgcg gctcttcttt cgggtatgga gataactcaa
     1141 gttacaaatc attcatctgc atctacttat gtgcctaaag ggagagatgc tactgtgagc
     1201 gatggaggat taaacttaaa gtttaaaaat ccatataagc atcctgtata tattaaaaat
     1261 tatgcaggtg gaggaagtgt atcatcagta atctatggta attctggtga caaaccaaat
     1321 atatctatag aggtaaaaca aactggtcag aataaatatt ctacatatcg aatatttaaa
     1381 gattcaagtg gaaaggtcat aaaaaaagaa catatatcaa atagttctta taaagaatta
     1441 aaaaaataga taatttttta gagaaatatt tgtaatataa aagggagtgt ctcaaaatga
     1501 actttttagt ttatgagaca ctctttttct gtgaaatcaa atatattttc atcatttcaa
     1561 caatacaaaa gaagcgtatc tctattttga gacagccttt tttgtaaaaa ttaacactat
     1621 tagttcttgt tttaatatta caatcattaa tatatagaaa atattcctat atattggtat
     1681 aattgtaaat agatttagtt atataaaatg tatgaggagg tgacatggtt gaattttata
     1741 ggcaatagat atgaggtagt aaattctgac gacgttttag aaattaacaa aatctataaa
     1801 gctagagatg ttttttacag gaaaaatgtg ttaataaaag ttataaagca caataattat
     1861 atctgtgaag attttgtttc aaatttaatt gatgaaagta ctgctttaga tgagataaac
     1921 tctccttata tactaaaaat aatagatgtt gggattcact gtacagaaga aacaacatta
     1981 tattatgttg taagtgaaga ctttaatggt atcggactag atgaattaat tctaggtaat
     2041 tatcttcatt tagaagcaat tgtaaacata atgatacaag ttttaaaagc tttagaaatg
     2101 atacatagga atcattcata tcatggtagt ttaaaatcga gtagtataat agtagataca
     2161 gaatacaata taaaaatttg tgattttgga attacaaaag caaataatgg ggttaataca
     2221 agaagctatg gaaacaggag atatttatgc ccacatcaac tatgtataaa ttatactgat
     2281 aaagaaagtg atttttttgc aacaggtctt attctatttg aatctatatt taaaaaattg
     2341 ccttttggag aatcaaattc agaagaaaaa atgctacaat ctattgataa gggattagat
     2401 tggaatagta taaaagcaat taatggaaat actgaactta taaatgttat aaaaaggctt
     2461 cttggaagaa ataataagta tactagagcc aatgatatta ttattgattt aagtaaagta
     2521 atgtatgaaa aagcatatat agaagttgaa gatgagaaag aagaagagca gaaacaagac
     2581 aaagttatac aagttaaaaa agaatataaa agtaaaatga gaaaaaaatt acataaaaaa
     2641 cttgttgttg ccggtctagc tatagtcatg atttcaatga ttattattag ttccataata
     2701 taaaaattgt atattaagta ttttaaaatc aatgtctata aaggatatac atagaaattt
     2761 tagtgtagtc ttaaattatg aaaggaagtg gttattatgt taacaaaaaa taatgaggta
     2821 ttaaacgtct aggagtattt taacaaatag ccacctgctt ttacagttta gaataaaaaa
     2881 gctgtggcta aaggtcacag ctttttgcta ctttttaaag gcaggagaat attaaaaagg
     2941 agcaaattat gagcaatata aaaataatag caagtaataa atgttggatt gaagatatag
     3001 caaggaaaca acttgaagat atttcaaagt taaatggaat tttaagagta gtaggattac
     3061 ctgacttaca tgcaggtaaa atacctgtag gtttagcagt tgagactaaa aatattattt
     3121 atccacatat aattggaaat gatataggat gcggaatgac tttatttaaa actggggttc
     3181 taaaaaagaa atttaaaaaa gacaaatgga ttaagtcact tagtaagatt aaagatttgt
     3241 ctgatataga aataaaaaac acatataaag aagaatgtcc aattttaaat ttaggaacta
     3301 tagggagtgg aaatcatttt gcagaaattc aatgtataag tgaaatttat aataaagaac
     3361 aatttgaaca attaaaattt tcatctgatg atattatgat gttagttcac tgtgggtcaa
     3421 gaaattatgg agaagatatt ttaaaaaaat tttatgataa agatggactt gaagttgaaa
     3481 gtgagaaagc tatagattat attaaaaaac atgataatgc tttaatgtgg gcagagcgta
     3541 atcgtgaagc tatcagtaaa aaaattatgc agtcaatagg tacatctgga gatgttgaaa
     3601 caatattctc tataaatcat aattttatag agaaaaggga agataaattt atacatagaa
     3661 aaggtgctgt ttcaagtgaa agaggagctg tgattatacc aggttcaaga ggcagtctat
     3721 catatatagt tatgcctact gaaaatacag agatatcttt atattctcta tcacatggag
     3781 caggtagaaa atggtctcgt tcagtttgta aatctcgcct taaaagtaag tatagtaaag
     3841 atactataaa acaaactaaa tttaaaagtc agattatatg taatgatgta aatttattat
     3901 ttcaagaagc tccagaggca tataagaata tagagcagat tattgaaagt ttgatagaat
     3961 atgaacttat tcaagtagtg gcaactatga agccattgat tacttataaa ggataatttt
     4021 taaagttaaa aaagattaaa attatattat aaatagctaa ttttatggaa catttaaaag
     4081 tgttttatta ttagctattt ttttattttg tagagacgat aatttataga gctaaattct
     4141 ttgatatata atgattacaa agttagactg tattggaaac tgcacataca tgaattgatg
     4201 acaatataag tgattcccaa atataaatga aagtataagc ttatttttac tataaaaatt
     4261 ttcactgtac aaaaacatta atgacattat ttttattgac ataaatatat aatttatgga
     4321 ataatattag aagcactgat ataagtgttt aaataagaat agaaaaggag aagtgtttta
     4381 tgaaaaaaaa tactaacatt aatgagtccg gagttaaact tcttaataaa taaggagttt
     4441 gatatataat aactccttat ttattaagtt gttcgcaaaa taactattat caaagtaaat
     4501 gatagggtga tttaaaagac gatgacttat aaataaagga gaaaaaataa tgaatagtaa
     4561 tacatttgaa agattacaac taaatgaagt taaagactta ataaaaatac attgtgtaag
     4621 ttcgctagga aaaaatttaa tagataaatt aactcctagt gggaatattg gagtagtaag
     4681 aaggaaatta gcagaaaata aagaagctag aaagataatt gaaaatagta atcacatacc
     4741 acttgaagga ttgtttaatg taggttctac aatagataaa attgaaaaag gaatgataat
     4801 agaacctgtt gaacttgtaa atattgaaga ttttttaaga ggttgtagga agatgaaagc
     4861 ttttatgtta gaaaaagaat tttattcacc tacattaagt tcttatgcct taaatataac
     4921 agaatgtaaa agtatagaag atgagataaa ttactgtata aaatctaata aagttgattc
     4981 taatgctagt aaagaattaa aaaagataag acgaaatata gaaattacag aaggaaagat
     5041 aaaagataga ttaaataaat ttataacatc aactgtaaat aaaaagtata tacaagagtt
     5101 tataataagt aaaagaaatg atagatatgt aataccaatt aaatcttctt ataaaaatga
     5161 agttaatggt actatattag atacatcatc aaaaggaaat actgtattta ttgaacctat
     5221 tagtgtttca aatttaagta cagagttaac tatgctaaaa gctgatgaaa ctattgaaga
     5281 atacaaaata ttatcttatt taacagaact tatatttgag aagataagtc agataaaatt
     5341 aaatatagag atactttcag aatacgatat ggtatttgca aaagctaagt atagtcaaaa
     5401 aataaaagga attactccaa aaataaataa taatggctat ataaaaataa ttaaaggtaa
     5461 acatccactt ctaacaggag atgcagtacc tttagatttt gaaataggca aaaattatag
     5521 aagtcttata ataacaggtc ctaatgcagg aggaaaaact gtaaccctta agactgttgg
     5581 tttactaact ttaatggttc aatgtggatt tgatatttct gcaaaagaag gtagtgaatt
     5641 tagtgtattt gaaaaagttt ttgtagatat aggggataat caaagtattg aaaatgcctt
     5701 aagtactttt tcatcacata taaaaaatat tgcagagatt atgagtttat caaataattc
     5761 tactcttgtt ctgtttgatg aaataggaag tggaactgaa cctaatgaag gagctggttt
     5821 agcaatatca ttattagaag agttttacaa aatgggatgt ataactatag catcaactca
     5881 ttatggagag ataaaaaagt ttgctacatt gcatccagaa tttgaaaatg ctggtatgat
     5941 gtttgataaa gaaacgttag aaccattgta taagttaact attggaaaat ctgaagatag
     6001 taatgctctt tttatttcta aaaaaatggg aataaaaaat agagttttag atagagctaa
     6061 agaatatgta attgatagaa actacaattt agatttagtt aaaagaagta agctacaatc
     6121 taatattgaa gataaaaata gttctgaagt cattttaaac tatacagatt ataatgtagg
     6181 ggataaggta aaattactag atgaagatga ttttggaatt gtatataaac ctatggacaa
     6241 gtttaataat gtagaagtat tgtttaatga taattttatt tcagtaaata taaaaagatt
     6301 acaacttagc ataaaagcat cagaattgta tccagaaggg tatgatttag atgtattatt
     6361 tactagtttt aaagaaagaa aattagaaaa agatattcaa agaggttcaa aaaaagctct
     6421 taaaaagatt cagaaagaaa ttagagagaa tagaaaataa atataaaaag cacaaaagaa
     6481 agcaatctaa agagaaaagt ttatctttag attgctttct tattaacata tcttcataaa
     6541 tccaatgact aacaagatta aaccacttaa ccaagataag tttaaattta cgtttttaac
     6601 aaacatattt cctagtaaac aaccaagtaa tactgcaatt atatttgata ttaaagaaaa
     6661 tagaataact tgtatataat tcacacctac aaggccacta ccaaaaccga tagctaatcc
     6721 atctaaactt aaagcaagtg caagtgtaaa tgcttcagca gatttaagca ctttagattt
     6781 acttgtatct aagacatcag gttcgatact tacattaaaa tttaatttaa ggtctaacat
     6841 attaaaatta taattctttg agtgtataga ttttttctta aaataagatt taaaaaaact
     6901 ttcaattagt ctagcacttc ctaataaaaa taaaataaag aaacatatta acattgttat
     6961 attttgaggt aaaaagtctc taactattgt acctgcaaaa agcgatattg ctaaaatagc
     7021 tgaacaaact acgctgataa ttatatttga atataaggga actttaactt tatttacgcc
     7081 atatccaaag cttgtcacaa aggcatctat acaaagagaa gtaactaata aaattgattc
     7141 aatcacagta gattaacatc ctttctctta aattttgtac ttatattagt tatagtattg
     7201 taaaaatgga taaattgtta cagataatcc aataaattgt tgataaaatt ccaacatcat
     7261 acaacaccta aataacaaaa gaatcatatc atataataat ataaaaccaa tcataataaa
     7321 gcgggtgaaa ataatggaac aaaataataa ggttgggaat ttcggtgatt tgttgaagat
     7381 tttattagat gaaaaagaat tatcaatggg agaactaagt aaaaaaagtg gaattgataa
     7441 atctactata tctcgtatag ctaataataa acaaaagcca aatataaatc atttagaaaa
     7501 gatggcaata catttaaata ttaacctaga agaactgttg aaagcttcag gatatgaatt
     7561 tagaaacagc aataatcaaa tatttaatgc agatagtgat tttagtaact ttgatgatat
     7621 tcttggattt gcaaatttaa tcaatgataa gaactttaat ggaaatattg aaaaagagtt
     7681 aagtaagtgt aagttatatg tccaaacaga tgaaggtaaa aaattacttt ttgataattt
     7741 taataaaaaa atagattcta ttgaaaaaca aggtcaattt acagatagat taagacaaat
     7801 gtatgctgat ttttgtacag atggacttac aataaagaaa tatttattga ttggaagtat
     7861 gctactttat tttgttatat caacagatgt gattccagac ttcgtattcc caataggatt
     7921 tatggatgat ttagtagcat taaatatagt tacaaaatta cttaaa
//
LOCUS       DAYASV010000160         7947 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYASV010000160 DAYASV010000000
VERSION     DAYASV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.165"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..687
                     /locus_tag="ACGI68_003340"
     CDS             <1..687
                     /locus_tag="ACGI68_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFH0139760.1"
                     /translation="SYISLDTINLIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFP
                     WSITSNGGVILKDNEILKSWSEKIDKLKCDYEPIESMIHKFKDYLNVDGITNFKVAED
                     TFFYIVVDLSRFNLDSIKEYTNILESKNWKFYVSGRKIYFIPKEISKENAIKYLTKEL
                     GIEYFYAVGDSIMDYGMLNISNKSYVLKHGDINKNEIENSFISSSFNGMSGTEEILSN
                     ILDENCLLNI"
     gene            complement(802..1356)
                     /locus_tag="ACGI68_003341"
     CDS             complement(802..1356)
                     /locus_tag="ACGI68_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0139761.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            1552..2754
                     /locus_tag="ACGI68_003342"
     CDS             1552..2754
                     /locus_tag="ACGI68_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0139762.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2811..3791
                     /locus_tag="ACGI68_003343"
     CDS             2811..3791
                     /locus_tag="ACGI68_003343"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFH0139763.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLSEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            4034..4987
                     /locus_tag="ACGI68_003344"
     CDS             4034..4987
                     /locus_tag="ACGI68_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFH0139764.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            4977..5933
                     /locus_tag="ACGI68_003345"
     CDS             4977..5933
                     /locus_tag="ACGI68_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFH0139765.1"
                     /translation="MKLSLNQKIYFVSILLFISMALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            5930..6685
                     /locus_tag="ACGI68_003346"
     CDS             5930..6685
                     /locus_tag="ACGI68_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139766.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            6777..7742
                     /locus_tag="ACGI68_003347"
     CDS             6777..7742
                     /locus_tag="ACGI68_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0139767.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAETLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         3094 a          818 c         1320 g         2715 t
ORIGIN      
        1 tcttatatat ctttagatac tataaattta ataaagcaaa tacaatatta tggtatgttt
       61 atacctacaa ctactagaac agtggagcaa tttaagcgta tagaatttaa taaatatggg
      121 atttattttc cttggagtat aacttcaaat ggaggagtta tacttaaaga taatgaaata
      181 ttgaaatcat ggagtgaaaa aatagataaa ctaaaatgtg attatgagcc cattgaaagt
      241 atgattcata aatttaaaga ctatttgaat gtagatggaa taactaattt taaagttgca
      301 gaagatacat ttttttatat agttgttgat ttatcaagat ttaatttgga ttcaatcaaa
      361 gaatatacaa atatattgga atcaaaaaat tggaagtttt atgtgagtgg cagaaaaata
      421 tattttatac caaaagaaat ttcaaaagaa aatgctataa aatacttgac taaagaatta
      481 ggaatagaat atttttatgc agttggagat tctattatgg actatggtat gcttaatatc
      541 tcaaataaat cctatgtatt gaagcatggt gatataaata aaaatgaaat agaaaattca
      601 tttatatcaa gttctttcaa tggaatgagt ggaactgaag agatactatc taacatttta
      661 gacgagaact gcctacttaa tatataatga ctgatatata aataataaac tagaaaaatc
      721 tgaaataaat tatagtaaag tttatcatac taatttattt cagattttta tcaatttata
      781 tttaagtaag aactcaatat gttaaaatga aggtggagtc attgcccaga ctgagataca
      841 tacctcatca tcataatttt catacacatg tggtatagta ctgttaaaat aaaaactatc
      901 tccttcctcc aatagatatt ccttatttcc aagtgttatc ttcattttac cttttattat
      961 atagccacat tcttctcctt cgtgtgtcac aaattcactg tttttttgtg tatgaccatc
     1021 tagtgtaaca agtataaatt ctattttttt cccagataaa ttaggaacaa gaagttcata
     1081 aaattttgta gaatcattag ttactatttt ttttcgatta tttttcttta ctactatatc
     1141 agagtcttct cttccaatct cttcaaaaaa ataacctata tttatatcaa gagcttttga
     1201 tattttccac attgctacaa tagatggaac aaccatatta cgctcaattt gacttattaa
     1261 gcctgtactt aaatctgaat tttttgctaa agtagagata cttatatttt tagattttct
     1321 aagagatcta attttgcttc caacatctat attcattaga tacaaacccc ctcagacatg
     1381 attataacat ataatttata aagattaata aaataaaaaa aaagttttac aatacaagta
     1441 ttttttatgc tatattgaat tttctgaaaa gatgcattat aatagtatta atattcaata
     1501 tattgaatat tgataacgat attttaattt aatgaaatgg aggtaattaa gatgaagagt
     1561 tttgtaagtc ctgataatat ttgggtatta tgggcagttg taactggatg ggcagcattt
     1621 agtatttatt tggaacaaaa gtacaattgg gcatcaaaag tgtcgggagc aataatagct
     1681 cttgtaggag caatgttatt atcaaatcta aatattatac cagtagagtc tgttgtatat
     1741 gaccaagtat ggggatatgt tgttccttta gccatagctt tattgttata tcaatgtaac
     1801 attaaaaaga tatggaaaga aagtggaaga ttgctcatta tatttcttgt aggttctgta
     1861 ggaactgtac ttggagctat gattggattt ttagcattaa aaaatgtagt accagattta
     1921 aatattgttg cagctatgat gacaggttct tatataggtg gaaatgtaaa ctttgctgct
     1981 atgtcagggg cgtttgatgc gccaggagaa ttagtatcag caacagttgt agcagataat
     2041 cttttaatgg cattatattt ctttgtctta atagctatac catcaattgg attttttaga
     2101 aaacatttta aacatccaca tgtagatgaa atggaaagta taggtataac tgaaggagaa
     2161 acagtagcag caaattattg gggaagaaaa gaaatatctt taaaagatat agcaatagcc
     2221 attggtagcg cctttataat tgttgcagta tctgtagaac tatcaacttg gtttaaagct
     2281 ataataccat tatcaaatcc ttttttagca atgttaaata ctttatttgg aaatcaatat
     2341 ctaataataa ctactttgac tatgttgttt gccacattta tgcctaattt ctttggtgat
     2401 ataaaaggag cacaggaatt gggtacattt ttaatatata tattctttgt tgtagtaggt
     2461 gttcctgctt caataacatt aataattcaa aaatcaccat tactactttt atactgcgga
     2521 ataatggtac taataaacat gctagttaca tttatagttg caaaaatatt taagtttagt
     2581 ttagaagaag ctattcttgc atctaatgca aatattggtg gtcctacaac agcagcagct
     2641 atggcaatat caaaaggatg gtctaagcta gtagggccaa tacttatagt tggaacattt
     2701 ggatacatag tcggtaatta ttttggtctt ttagtaggaa atattttgat ataattttac
     2761 gtgaaaaaaa gttgtttatt agaaagattt ttatttaatt ggaggttgtt atgattttcg
     2821 atggacattc agatacttgg gcagatgtaa caataagaag gcttaacggg gaaaaagatg
     2881 tatttagaag aatccatctt gataatttta aaagagcaaa tatagaagga gctatttttg
     2941 taatatggat agacccacca tatgataaag agcctgaaaa aagagttaaa caaattataa
     3001 gttctataaa agatgaaatt gaagatatga aagatgttgt aaacatagta aaaaaatatt
     3061 cagatataga taaaggtata agtgaaaaca aaataaatat tttaattggt cttgaaggat
     3121 taagccatat tggaagaaat atagatttac tagattattt ttataaagaa gttggtgcaa
     3181 gacatgcatc attaacttgg aatgaacaga atgaacttgc tacaggatgt ttaggggatg
     3241 aaaatcgtgg tttaagtgaa ataggcaaaa aagcagtcaa taaaatggaa aaattgggca
     3301 tgatagtaga tgtgtcacat ttaaatgaaa aatcattttg ggatttaatg tctgttgcaa
     3361 gtaagccagt tatagcttct cattctaatg ctaggagttt atgtgatgct aaaagaaatc
     3421 ttacagataa tcaattaaaa gaaatttcaa atacaggtgg attagttgga ataaatagtt
     3481 ttagaggatt tactaatagt aatcctaaga agcaagattt aatacatttg gttgaccata
     3541 tagactatat ggtaaatctt ataggtatag aacatgtatc ttttggtttt gattttagtg
     3601 attacttagg gaaagagact ataggttctt ttactggaaa tgctagtgtg ccaagtgtag
     3661 atggtcttga aagcacacat acagcacaga acatagtagt agagcttgaa aatagaggtt
     3721 ataaaaagaa agatatagag ttaatctcta gtggtaattt ttataggctc tttaaagata
     3781 tactaaaata atataataaa gaacttaaat ttataaaata gtatatttat acaaataata
     3841 tctacatatt taaattggcg ttcataattt attaaatttt aatgttacta gaagtttcaa
     3901 ttctagtaac attttttatg ttaaaaacag atatagatat gtattgacaa tgataattat
     3961 tattgataaa ctataaaagt gaaaatgata ataattttca ttttaattaa taaaagattt
     4021 ttaaggaggg agaatgaaga aaaggtattt agtcatagga ataataattt tgtcttattt
     4081 ttctttattt ataggagctg aagatataaa tataatgcat atatttgcca aaaatcagca
     4141 taaacttatg atatttatca tgagtagaat accaagatta ataagtatat tgattgctgg
     4201 tgtaggaatg agcatagcag gtcttataat gcaacagata agtaagaata aatttgtatc
     4261 acctacaaca ggtgctacaa tagatgcagc acaatttggg atagtaatat gtatgctttt
     4321 agtaccaact gcctctattt ttacaaagac aataatagca tttgtatttt ctcttgtagg
     4381 gacttttacg tttatgaaaa taatagggaa gttacaattt aaaaatataa tctttgtacc
     4441 attagtaggt ataatgtttg gaaatataat agggtctatg acggatttta tcgcgtataa
     4501 atacgatttg agtcaaaatg ttagttcttg gatgcaaggt gacttttcta tgatacttaa
     4561 aggaaattat gagatattat atatcacaat accccttata atattagcat atatttatgc
     4621 aaataaattt actgttgttg gtatgggtat ggattttgcg acaaacttag gtcttagtta
     4681 caaaagaatt gtcaatatag gtctgataat agtagcactt gttacagtat gtgtagtcgt
     4741 tacagctggt aatatccctt ttataggtct aatagttcca aatatcgtat ccttgtacat
     4801 gggtgataat ataagagcaa gcatatggta tactggttta cttggagcga tatttgtact
     4861 tatatgtgat atttttggaa gaattattat ttatccatat gaaatatcta tagggcttac
     4921 agttggagtt ataggaagta tactattcct ttatttaata ttaaggagga atgtgaatga
     4981 agcttagtct caatcaaaaa atttactttg taagtatatt actattcata tcaatggcac
     5041 tttttataat ttatggaata gacatgaatc atttagaata tgcattatca caaagaatac
     5101 caaaaatatt tgctatggta ttaggtggag gatgtatagc atttactact gtagtgtttc
     5161 aaacaattac aaacaatcaa atattaacac caagtgtact aggtttagat tcattgtatg
     5221 taatgattca aactataata gtattcatat ttggttcaag cagtagtctg attataaatg
     5281 aaaattataa ttttattatt aatgtagcta tgatgattgg agcttcattg ttattatata
     5341 agacgttatt tgaaaaaaat aaaaataata ttttcttttt aatcctagta ggaatgatat
     5401 ttggaacctt atttaaaagt gcaacaacat ttattcaggt aatgatagac cctaatgaat
     5461 ttttagcttt acaaactagt ataatggcaa gtttaaacaa tataaacact aatgtattgt
     5521 taatagcatt tataataatt attgccataa taccttttat atatgatgaa ataaagtatt
     5581 tagatgtatt gtcgttaggt aaagaacaag caattaattt aggtgtagat tttgataaag
     5641 cagttaaaaa aatgataata ttaatagcaa ttttagtctc aatatcaaca gcacttattg
     5701 gacctatgac atttttagga cttttacttg ctaatataac aagagaaatt tttaaaacat
     5761 ataagcatac atacttgatt tcgggttcta tgttgatagg gatgattaca ttgataatag
     5821 gtcaattttt tatacaacat gtatttaagt ttgatacaac tttaagtgta gtaataaatt
     5881 ttataggagg aatttatttt attcaattac tattaaaggg ggcaaataga tgatagagat
     5941 aaaaaatata tttaaaagat ataagaataa aaatgttgta gatgatgtct cttttagtat
     6001 agaaaaggga aagataacat cttttatagg accaaatgga gctggaaaaa gtacagtact
     6061 atcaatagtt acaagactta taggaggtga tggtggagaa gtaataatag aaggaaaaag
     6121 cttgactaat tatagcaata aagaattagc aaaaaaaata gctattctta aacaatctaa
     6181 caatataact ttaaagctta caataagaga attagttgga tttggaagat ttccatatag
     6241 tgaaggtaat ctaactaaag aggatgaaaa ttatattgat gaagcaatag aatatatgaa
     6301 gttgactgat atacaacata agtatttaga tgaattgagt ggaggtcaaa gacaaagagc
     6361 ttatatagca atggtaatag ctcaagatac agagtacata ttacttgatg aaccactgaa
     6421 caatttggat atgaaccatt ccgtacagat gatgaaggta cttagaggtc tttgtgatga
     6481 attagataag actatagtat tagtaatgca tgatataaat tttgcatcat gttattcaga
     6541 taatatagtt gcattaaaaa atggaaaagt tgaaaaagta ggtcgtacag atgagatagt
     6601 aaatgaaaaa gttttagaag atatttatga gatgaatttt aacattaaga atataaatgg
     6661 aaatcgaatc tgtatttatt tttaaaatat tgtatatgaa aataaacttg ctatatgatt
     6721 ttttataaag tttttaaatt aaaataaaga ttaataggag agaggaaaat attgatatga
     6781 ataaaaaagc agcaatagta gcagcagtag ctattatagg attagtgaca gtgtttgcat
     6841 taggtggaag taaaaagaat gaatcaaaaa catcagaaaa ttcaaataat actatgaaga
     6901 taactcataa cttgggagaa acagatgtaa aattaaatcc taaaaaagta gtggtatttg
     6961 attattcggc tttagataca atggatgcat taggtgtagc tgagacttta gtaggacttc
     7021 caaaagcgag tttaccagca tccttagaaa aatataagga tgacaagtat gcagatttag
     7081 gaggattaaa agaaccagat ttagaaggta taaagtcggc aaatccagat ttaattataa
     7141 taaatggaag acaagaagat ttttatgagc aattgtcaaa aatagcacca acaataagta
     7201 caagtaaaga tgataagaag tacttagaat ctgttaaaaa taatatagac aaaatagcaa
     7261 aaatatttgg agtagaagaa aaagcaaatc aagaatttag taaaattgaa aagaaaatag
     7321 aaactttgaa taaaaaagta acagataaaa atttaaatgc tttaactata atggtaaatg
     7381 aaggtaattt aagtgtattt ggtgaagaat caagatttag tatattatac aatagttttg
     7441 gatttgaaaa taaagataaa aatataaaag aatcaagtca tggacaaaat attacttttg
     7501 aatatatagc taaacaaaat ccagaagtta tgttcgttat agatagagga atagctactg
     7561 gcagtgatgt aaaagaaagc tcaactgcaa aatctgtttt gaacaatgat ataataaaat
     7621 ctatggatgc atataagaat gataatataa tatatttaga ttcaccaaca tggtatgtaa
     7681 atgatggtgg tctaacatct ttaaataaaa tgatagatga tgcttcaaaa gcggtaaatt
     7741 aataatccga tataaccaaa atgtattttt tataaaatga ttatcaaata aactaggagt
     7801 ttttaagaac tcctagttta ttttttgcat aaaaaagata tagtttaaac aataatatta
     7861 tctataataa gataaatcta ttcaaaatct aataaaatat gaagttatac tagacaaaaa
     7921 gaataactct taaatataat taagtta
//
LOCUS       DAYASV010000161         7809 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYASV010000161 DAYASV010000000
VERSION     DAYASV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.166"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            365..742
                     /locus_tag="ACGI68_003348"
     CDS             365..742
                     /locus_tag="ACGI68_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139768.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            735..1883
                     /locus_tag="ACGI68_003349"
     CDS             735..1883
                     /locus_tag="ACGI68_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0139769.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1900..2502
                     /locus_tag="ACGI68_003350"
     CDS             1900..2502
                     /locus_tag="ACGI68_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HFH0139770.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2595..3617)
                     /locus_tag="ACGI68_003351"
     CDS             complement(2595..3617)
                     /locus_tag="ACGI68_003351"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFH0139771.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3946..4128)
                     /locus_tag="ACGI68_003352"
     CDS             complement(3946..4128)
                     /locus_tag="ACGI68_003352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139772.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4441..4860
                     /locus_tag="ACGI68_003353"
     CDS             4441..4860
                     /locus_tag="ACGI68_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HFH0139773.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4991..6832
                     /locus_tag="ACGI68_003354"
     CDS             4991..6832
                     /locus_tag="ACGI68_003354"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0139774.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6916..>7809
                     /locus_tag="ACGI68_003355"
     CDS             6916..>7809
                     /locus_tag="ACGI68_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0139775.1"
                     /translation="MLTKFYLNLTDLNYILDYHKDLLNTKLEDNSRVYTLYLISLINY
                     IKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLTLSAISIRKYDKVNNFYFSA
                     KKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVEEVSFFKTLKNKALVSRANY
                     IFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFCKCIISLCYLESGNNVEAIK
                     YINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKEAEVILLKTIKLMKVVEGVY
                     KDYLYSFIFLYSAEIELKKDNC"
BASE COUNT         3192 a          812 c         1120 g         2685 t
ORIGIN      
        1 tgtaaataat cagtataaaa tagatgtttt aatagcataa aggcataatt agaatgttat
       61 taaaatttat tagatattac tctagtttat tttaaaaaaa agtattataa tattccttgt
      121 gtgaaaatat aggaattttt attaaatagt tttaatgtgt aaataacatt ctacactatt
      181 gtaattaaat atgtaaaaag taaaatattt aaatagatat attttaaaat ctaactttta
      241 atattgtaaa aaaattagaa atttaacaaa aagtacaaaa attcacattt ttttcataaa
      301 gtgtttgact aaaaaattca catgttgtag aattaaggta gcaggagttt tgagggaggt
      361 gtaaataatg ataagaaaaa taccacaagc agaactaaaa gtaatgaaat tcatttggaa
      421 agtagatgtt acagtgacat caaaagatgt tattgaagct atggaacaaa aatatggctg
      481 gaaacaaacc accacactga cacttttatc taggctggta aaaagaggat tcttagatgc
      541 tcaaaaaata gacagatata cacattacac aataagagta aaacacaaag attatttaag
      601 ttttgagaca aaggatttcc taagtaatat acatgatgac tctttaagaa gcttaatttc
      661 agcattacat gatgatgaaa atataggtaa agataagcta gattttctag aaagttattt
      721 tgataattta aaggatgaat aattatatgc tatatgtttt tgaaaatctt ataaagacaa
      781 ctattgtatg tagtcttggt atttgtctat tattattttt gaaaagatat ttatttaaga
      841 agtttagtaa gagattcaat tattatattt ggttgataat agtctttaga atgttacttt
      901 ttctatttaa ttatacaata gtgtatgaag taaaagaacc caaagaaaat gcattaggaa
      961 ataatattac acaaattgat atttctacag ataataatct tatgctgtat gtagcttatt
     1021 tatggttatt tgtaactata gtcattgcag tttatacatt tataaagtat acaagattta
     1081 aaaatctagt tgttgatgta tcttatgata ttgaagataa tgatataaac tgtatctaca
     1141 aaaacttgtt aaaagaatta aatataaaaa agaaaattga attaagaggt tctgatgaat
     1201 tgataagtcc agcaggtatg ggattattta aatcttacat attcttacca gactatcctt
     1261 atagtaaaga tgaactaact tggattttga aacatgaatt aatgcatttt aaaaataaag
     1321 atattttaat aaagttttta gttttgtctg ttagaataat atattggttt aatccacttg
     1381 tatatgtaat gagcaataaa gtaaatctag attgtgaatt atgttgtgat gaaagtgttt
     1441 tggtagaatg ttctttaaaa gataaaaaag aatatgcact agcacttata aagtcaatta
     1501 agtttagtaa aaattataat agtaagattt tgacaaccga gtttgataag acaaatttgg
     1561 aaaagagact tgagagtatt gttaaaaaga aaggcaaaag tggaatacta attgctgttt
     1621 tagtatttat gacttctata acatcttttg ttgaagttga tgctcaagtt agaattgatg
     1681 agggtaataa tggaatgaat aacaatatta atgaattttt attgaataag acgactgata
     1741 aaattataaa tatttattat aaagatgcac caaatgaaat taggaaattt tatgaagata
     1801 aatgcgaatt agaaggtaaa atacctagag actatgacat aatagaaatg gatatgaaag
     1861 attataatca attaataaaa taattataag gaggatttta tgttaacaaa attaagaaaa
     1921 atagtagcta ttatgtgttg cttattgctt acttttacaa gtgtggcatt tgctgacaca
     1981 agtaaagaga aaggaggaag taaagagact gttccagaga aaaacctaga ttcttttact
     2041 ctttctgggt atatatatac atatgaaaat gcgccacaag aaataagaga tgaacataaa
     2101 caaaattgtg aagagataaa tatagaccct aagccagatg atgagatatt tgttccagaa
     2161 tcagctaatc taatgaatga ggacaatagt aaaggtgttt atgcaagtta tactgtttct
     2221 tataatatag gtgctaaaac tataacaatt atgagtaatg agtatttgac tataagtaca
     2281 acaaaagtta tacgaaaggg taatagcgga aaagaagtaa aagctgcaca aatcatgtta
     2341 actttacttg gttataatgt aggtatagat agttcttttg gaagtaagac atataatgca
     2401 gttgtaagtt tccaaaaaaa atacggtttg tctgcagatg gaataattgg tccagctact
     2461 tgggataaat tgggtagact aactgaccct actctttcat aggattaaaa tctaaaaata
     2521 tattaaatag tgtctcaata tgatttttca atttaaatga gacactattt aaatataaat
     2581 gttataaaac tattttacaa taaattccat ataactctac catctatatc cttaacattt
     2641 tgacaaccag tcaaaatcat agtctcacat aactcattcc taaccttctc tataaaaaac
     2701 tccacaccat caagaccacc gccaaaagaa gcagtaacaa aaggtcttcc catcaaaaca
     2761 gcatcagcac ccaatccaag catcttaact acatcaacac cagtcctaac tccaccatca
     2821 acaagtattg ttatctttcc ctttacagac ttagctatat ctggaagtac ctcacaagta
     2881 ccaggagtat aatcaagtac tcttccacca tgatttgaaa caataatggc actagcacca
     2941 gactctacag ccatcaaagc atcatctaca gtcataattc cttttataat aaaaggtaat
     3001 ctagtggatt caaccaattc tctaatctca tcaattgttt taggagaaaa aggattttct
     3061 tgaaattgat tattaataag tccacaagca tcaaggtcta caccaactgc aaaagcacct
     3121 gcttcttcag acaatctaat tttatctatt atctttgaat tattccaagg ctttataaat
     3181 acaattccat ttcccctatt atccttcaat acatctaaat tatccagtaa aaaagtatct
     3241 acattagtat cgccaaccat agcataaatt ccagaattag aacaacctct aacaacaggc
     3301 tctatgtatt ctttttcgga aactttacca cccatgttca atatagtacc tgatacaggt
     3361 gcagcgaata taggagaact catttttcta ccaaataatt ctatggatgt atctggcttt
     3421 gaaacattat gtatgactct catatttatc tttatttttt ctagactttt tctattttca
     3481 atgaaagacg aaccacttcc tttaccaccc ataccaggaa catctccagc acaagctaat
     3541 ccattacaaa ttttacatac tttacatgtt ccattaaagt tctctctagc tgattttagt
     3601 aagtcattgt aatccataca aaatctctcc tttttataat attaaaattg agtattacta
     3661 tattgaaaat atcttaattg taaatatagg gatgttatta tgatgagcta aactagataa
     3721 tacatattag atatttttta ttgttgtaga tagttaaatt atatcatatt agatagtcaa
     3781 attttgaaaa aaactaactt atttaagtat acattttgaa aaaattaaaa aagctatata
     3841 tagattttta aagaaatcta tatatagctt ttaagtttat gtcaatcttt tttgttaatg
     3901 aataaataat atagaataca atatacaaaa aagtttcttg aaatgctaag ataatcccca
     3961 tttacaaagc aacatcatta tactgtgtaa atttgaatca acaatgtttt caccatattt
     4021 catcataaaa tcctctaatt gtgtgtcaat aaatgaataa tatccactta tactatcgat
     4081 gtatgcagtc attaagtgta tacattcatc acttttatct ttatacatat atatctcccc
     4141 ctatatacaa tatataaatt tagataccaa atcgtatcat attaaatagt taagtgtgta
     4201 acatctatgc agttatatat ttatatcatt atattaataa ctaaagtgag ttttgacaat
     4261 agattttggg aaactggatg ttcaaagtgc aaacttggat tttaaaatca ttaattaaga
     4321 tagcgcatag gaatatatgt atacataact taattataaa gattataaac aattactttt
     4381 attaacttta tttaaatggt agaataactt ggagattaat aattataagg aggatataat
     4441 atgagtttat ataagtttat atatgatgat aaggaatatt tattaaaaga ggagaactgt
     4501 agtgctttaa taaatgatga agaaaatcca gttcaaggag taagtatttc aaaaataatt
     4561 gatatactta atgaagcaga agaagttgat tttgatgtag aatattatca agaagcatgt
     4621 ccattatgtt tagaaggagt aaaagaaaag aaaaaattct ttcctttttt agaatatcac
     4681 ttttatattt tcagtaaaga tggagaatat gtaataagca atatatctgt tgattataaa
     4741 ggactatctt ttaataaact atcaagggca aataaagttg ataatagtta tattgtaagt
     4801 gtgataattt gcgaaaattg tcaggattat atagttcaga tagaaaattg tatagtttag
     4861 tgtaaagtat gtcttaattt aagagaaatt tctaaattga gacatcaact tttttcaaaa
     4921 atagtatgga aaattactaa atttatgaga taatgtaagt cggaggtgag ttacaatata
     4981 tagtaaatat ttggaagaga tagatgatac aattttagat tcaatgttta aactaacctt
     5041 gaatgaaaga ttattgacta ataacgattt tattgaaaat aaaattgatg tagtaaagaa
     5101 tttaattgat tgctataaaa ctgaacaaaa tagtttaatg ttattaaaat caaatttttt
     5161 gctagcttta ttatatggta ttcaagggtt tagtgaaaaa atgaaagagt atattttgat
     5221 ttcctgtaaa tatattgata aatgcttacc caaagattat aaatttttag cacgatttta
     5281 ttcacaaata gctattttaa gtttaaaaaa tgaagatgta aataaggcaa atttatatat
     5341 ggataaattt aatcttattt gtgatgaaaa taattttcta attgaagaaa ttatttttaa
     5401 atcacaactt atttacatta aggcaagtaa gtgtatagaa tcttctgtaa taattaaaga
     5461 aatagaaacg ttgtatataa agataaaaga aattaacgat tttaattgta aaaaaatata
     5521 tttttttatt attggtaaaa tatatttttt gtttttggat gatgctctaa ttgctaaggt
     5581 aaatttttta aaagcaagaa aatatgcaga tttactaaag gatatgcaag tatcttcact
     5641 atgtaatata aagataggtg agtgtgaatg ttcgtttgaa aattatgaat gttccatgga
     5701 atatttcaat gaagtaataa gcaataaaaa gtatagaaat gtaaatataa ttcaaaaata
     5761 tagagcatca aacaacataa ctaaaatatt aataaagact agaaattatc ctgtcgcaat
     5821 aaataattta gcaaaatctg aagtatatct agaaaaaatt aaaaatcata gcttaaaaga
     5881 agtagagaag tttgatttat ttataagttt agccatgtat tatgcaaaaa gtaatgaaaa
     5941 atcttttgaa caatcaatat gctatttaga tagagctaaa aacatattaa atatgataaa
     6001 aaatatagaa gaatatataa tatatgattt agaaataatt tatcaccagg caaagatata
     6061 ttatatcttt ggatattatg acaatgcctt aaatacaaat aaaaaactcc ttgaaaaatc
     6121 taaaaatgct aaaaattata attatgtgaa gttagcatac aagagtatat atttgtgttt
     6181 tgaaaagatt caaaattatg aaatgagtat gaaatacttt aaaatgtatt atgagttaaa
     6241 aatgatgtat ataaaagctc gaaatagaaa ttatatagat tccttaaatt atagacatga
     6301 gtatatagaa aaagaaataa ataacatgag aaaagtaaaa cttgatttag ataaaaaaaa
     6361 gtatgttgac catttgacaa gtgcatataa tagaactttt ttgaacgatt ttttagaaaa
     6421 acaagaagtt agtgaatatt gcacagcatt tatgatagat gtagattact ttaaagaata
     6481 caatgatact tatggacatt ataatggaga tattgctctt aaaaatataa ctataataat
     6541 aaaaaaatat ctaaaaaaag acatgcttat gataaggtat ggtggagaag aatttctgat
     6601 attatctgta tgtaaagatt ataaaaaaag taaaatattt ggaagaaaat tatgtaggat
     6661 tgtaaagaaa tttttaaata ataatttgac agttagtatt ggcattgata catgtaaaaa
     6721 tagtagtata gatataaaag aaataattga aaatgctgat aaagctttat ataaagcaaa
     6781 ggaaagtggt agaaatagat gtttgcatta tcatgatttt aagaattttt aactgttgaa
     6841 ataggattgg tgatttactt gaaaaggagt ttaatagaag atttaaagag aaatataaac
     6901 aagatagaat acgatatgtt gactaaattc tatctaaatt tgacagattt aaactacatc
     6961 ttggattatc acaaagacct tttaaataca aaattagaag ataatagtag agtctatact
     7021 ttatatttaa ttagtcttat aaactatata aaaaaagata gagaagcatc atacaactat
     7081 ttgatagagt cattaaagta tattaaaaaa ttgtcattaa aagatgattc agatataata
     7141 tctaaaattt atatttatct tactttatca gctatttcta taagaaaata tgataaggta
     7201 aataattttt atttctcagc aaaaaaaatt ataaggcaaa atcatttaaa tgagttgttg
     7261 gtattgttaa atatgagttt atgtgtggaa ataagtgctt tatatcagac caaaacggat
     7321 gtaatagctt tagtagaaga agtgtctttt tttaaaactt taaaaaataa agctcttgta
     7381 agtagggcaa attatatatt tgggagagtt tatctatatt tatttaataa ctttataaaa
     7441 agtatggaat acttaattga atgtctaaaa ttggctcaag aaaataattt atactctata
     7501 gaggcttttt gtaaatgtat aataagtttg tgttatttag agagtggaaa taatgtagaa
     7561 gctataaaat acataaataa tgtattagat gttgcacaca gtaataactc aatatcagta
     7621 actgaaagag tgtctataaa aatagacctt atatgggcat atttaaaaga agatatgaat
     7681 aaagaagcag aagttatttt attaaagact ataaaattaa tgaaagttgt tgaaggtgta
     7741 tataaggatt atttatattc atttatattt ttatattcag ctgaaattga gttaaagaaa
     7801 gataattgt
//
LOCUS       DAYASV010000162         7715 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYASV010000162 DAYASV010000000
VERSION     DAYASV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.167"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            322..1623
                     /locus_tag="ACGI68_003356"
     CDS             322..1623
                     /locus_tag="ACGI68_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0139776.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1699..1974
                     /locus_tag="ACGI68_003357"
     CDS             1699..1974
                     /locus_tag="ACGI68_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFH0139777.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3309..3539
                     /locus_tag="ACGI68_003358"
     CDS             3309..3539
                     /locus_tag="ACGI68_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139778.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3532..4113
                     /locus_tag="ACGI68_003359"
     CDS             3532..4113
                     /locus_tag="ACGI68_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFH0139779.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(4692..5369)
                     /locus_tag="ACGI68_003360"
     CDS             complement(4692..5369)
                     /locus_tag="ACGI68_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0139780.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5558..6922)
                     /locus_tag="ACGI68_003361"
     CDS             complement(5558..6922)
                     /locus_tag="ACGI68_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0139781.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYVARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTVFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7050..7571
                     /locus_tag="ACGI68_003362"
     CDS             7050..7571
                     /locus_tag="ACGI68_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0139782.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         3015 a          880 c         1328 g         2492 t
ORIGIN      
        1 gagtaatgaa ttatattgaa cgatatatca ataattggca gtaagttata tcaaataaga
       61 tattactatc gagtaatgaa ttatattgaa caatatatta ataattggca gtaagttata
      121 tcaaataaga tattgctatt atgtagtaaa ttatataaaa caatgtatca gaataaatag
      181 tatattaata atttgtagta tatcatgacg aataatacag tcataacaaa tagtgagtca
      241 taacaaataa tgtatttata attgataatg tatttaaaaa tcaaaatata aaaacttata
      301 aggaaagaga gattaattta tatgggtaaa tcaaaagatg ttgaaataga gaagtctatt
      361 ttaggcacaa ttcttttaga caataaatta agctataagc tagatgaatt aaatgaaaac
      421 atgtttatga atgatatatg tcttgagata tttaaaatta tgaaagaact taaaaaagaa
      481 aacatagtga ttgatgtggc cactgtgaaa tcaaagatag atagaaaatc tctagcaata
      541 aaaacaagtg atgtgactaa tctaattact tggggtcaga attttggtct tgatggtcat
      601 attaaaattt taaaagaaaa tttagctaga agaagcataa atcaaaactg ccaaaacctt
      661 ttacacagcc taaaccttgg agaaaatatc gatacttgta tttacaaatt tgaaagtaat
      721 ataaaggaga ttttggacaa ggatacttat gaaaatgatg atgtaaatag tatagcaggg
      781 aaggtgcttg attttttaga aaacaaaaaa gatattggat ttaaatttgg tataaaatta
      841 cttgatacca ctataggagg tctttttaaa ggagagctaa ccacaatagc agctaaatcg
      901 ggggttggga agactgcttt ggcacttcaa ataatgttaa attcatttaa gcaaggtaaa
      961 aaaactttat ttataagtag agaaatgacc agtgagcaag tatttatgag aaatatatgc
     1021 agagtaactg gtgtaagtac aaggaatatg aaatctaaag aaatagatga aaatgattgg
     1081 aaacttattg taaatgcgat aggtgattta agcgaaaata acttaattta tatcaatgat
     1141 aagatagata caattagtgc aattagaaag aggataaggc aggtaaagcc agatttatta
     1201 atagtggatt atgtgcaact ccttacatct cagaaaagca tggataagag agaaagggag
     1261 gtagctactt tttcgagaga acttaaaaat atgactttag attttaatat acctgtaatc
     1321 caattaagtc agttaaatga tgaaatgaaa gattcaagac cttatggaga tagacctatg
     1381 agggatagca aagccatcta ccacgatagc aacaatgtgg tttatataca tcaattaaag
     1441 gggagtgatt atgaagaagc agtgagagat attggagaaa gtgaggaggc agttagagca
     1501 tcagaataca ggggaattaa gatggttgat ttaatagttg cgaagtgtag agatggacag
     1561 actaggcata agcatttttg ttactttggg gataagttac attttcaaga actaaattat
     1621 taggtcaata aagtagtaat aacaagctta ttaaaagtcg ggaaatagca aatagattat
     1681 aaaatatgag gtgaaaatat gttggaatta agaaaaatta tagaattact tgaagaacac
     1741 aagatagaag taagtattcc aaaggagtta cataagtata gtttattgga ataccttgaa
     1801 ttgtgtcaac aaagtggggg aacatatttt tatattaaaa cactggaagg ggctttaaaa
     1861 gaggctagaa atgagcttat gattaaattg tttaatggga aaaattatat ggaattagcg
     1921 aggatgtttg aaatgagtgt tattaatgtc aggcatattg ttagaaaaaa atagatattt
     1981 ttggagaata caagtgtgat atacttctga aatagaagtt attataagag gagtataagt
     2041 gtgattaaca acatgatttt gtgaataaaa ttgaggatat atatgagtta aatgtgtgat
     2101 taataaggta agattgtgta taagattatg agtatttaaa aggaggggct ataattaata
     2161 gtgtagagtt gtgtgtttgc ttaagattat ataataattt aatagattaa actatactaa
     2221 taaggtaaaa tcgtgtgttt agttaaagct tattataaaa tcttgatatg tgattaatga
     2281 tgcaatatgt gaataaagtt aagattataa tagatttaac ctgtggctaa tagaataaaa
     2341 ttgtgaataa agttaagatt ataataggtt taaattgtga ctaatagagt aagagtgtaa
     2401 atagagataa acttcaatat gagcttaacc tatgattaat agagtaagaa tgtaaataga
     2461 gataaacttc aatatgagtt taacttgtgg ctaatagagt aagagtgtaa atagagataa
     2521 acttcaatat gggtttaact tgtggttaat agagtaaggt gtaactaaag ataaacttca
     2581 atatgagctt aacctatgat taatagagta agaatgtaaa tagagataaa cttcaatatg
     2641 agtttaactt gtggctaata gagtaagagt gtaactaaag ataaacttca atatgggttt
     2701 aacttgtggt taatagagta agaatgtaaa tagagataaa cttcaatatg agcttaactt
     2761 gtgattaata gagtaagagt gtaaatagag ataaacttca atatgggatt aacttgtgat
     2821 taatagagta agagtgtaac taaagataaa cttcaatatg agcttaacct atgattaata
     2881 gagtaagaat gtaaatagag ataaacttca atatgggttt aacctgtggt taatagagta
     2941 gggtgtaact aaagataaac ttcaatatga gtttaacttg tggctaatag aataagattg
     3001 tgaataaagt tatagtttta gatgggtatt aacctgttac taatagaata tgattaggtg
     3061 taaaattata gagctgcata agaaaatatt atagatatat taaatattag ataataaata
     3121 tttatataga tacacaaaag aaaaggcgat atgaagctaa acttaatgac ttcatatcgt
     3181 tttttatttg aaactccttt gtatttagag tgtgattttt aatattgagg ttttatgtga
     3241 ttaagtcaca gataaaaagt aagtatcttg atataattct tatagattaa taatataggg
     3301 aggttattat gtctaataaa aaaaagaagt atgcaaatat tttagcaaat gagtgcgttg
     3361 cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat ctcagttcca tgtggcatta
     3421 gtgcaaaaat taatagagat ttatgtgttg gatgtgggat ttgtgaaaag atatgtccag
     3481 catctgtaat tgagattata acaatattaa aagaggaaga aggtaagtgt catgaataat
     3541 aaaaaacatt ggtatgacta tttatggatt ttttcttcaa tatatttgat attgggattt
     3601 gtaaatatta ttttcgcttg gattgggcta atttgtttct ttgttccact agctatttct
     3661 attgttaagg gaaataaagc ttactgtaat aagtattgtg gaagagggca actgtttaat
     3721 ttgcttggaa ataaattgaa gctgtcaaga aatagagata ttcctaaatt tattagaagt
     3781 aagtggttta gatatgggtt tttgacattt tttatggtaa tgtttgccaa tatgcttttt
     3841 tcaacttatt tagtgtttga aggaagtaga aattttaaag aagtagtgac tttgttgtgg
     3901 actataaaaa tgccttggca atgggcgtat catggaactt tagtttcacc ttgggtggta
     3961 cagtttgcat ttggttttta tagtgttatg cttacatcaa ctgttcttgg tttgattact
     4021 atgatattgt ttaaacctcg ttcatggtgt gtttattgcc ctatgggtac tatgactcag
     4081 atgatatgta aggcaaagag taagtctgta tagaaaggcc ttatgtgata tttacagttt
     4141 tgttcaagta gagatgagta gtaggtttga atatatatga caaatatatg agtatagggg
     4201 catagattta gtttgtgtaa ggtaaactga tttatgcttc ttttttatgt cttagattat
     4261 tggctaagtg gaaacaatga atttagatat taagaattat tcagttattt gaagaaaata
     4321 gaataataat taacattata ttaatggata ttcattacat ataagccagt taatagcaag
     4381 atttatttaa tatgatatag gtcaagtatt taaactaaat aaggtcatga caattaatat
     4441 aagtatagat ataaaaagat tagaagttat aacttataaa aggtattcta tataaatagt
     4501 atggggaaaa tagtcaaatt aacttattag aaacaaaaaa tatacataaa aaataaatgt
     4561 aaatatcaaa taagatacta tatcaaaata gatataagta tcttttttat tattgaaatt
     4621 atgaaaatat atttgatttt acacaaaaaa gagtgcctca tgaactaaaa agttcatttt
     4681 gagacacact tttacagtgt caaagaacga agtttatcct tatccaatac ctttatacca
     4741 ccacgagata aagacacaat accttctcta gcaaaatatt tcaacattct cgatacaact
     4801 tctctggcac tacccatata cttagcaatt tgctcatgag tcatatttat agtgctactg
     4861 ccatttttag ctatctcatc aattaaaaaa gatgccaaac gcttatcaaa actcataaaa
     4921 agaatttgtt gcatagccca cataacatca gaaaaacgtt cagtagctaa tttatatgaa
     4981 aataactctg catgaatatt ttctgtagat aattttgcaa aaacagagga actaatctgt
     5041 ataatatcgc agtccgtttc agcatctaca tagacatcaa aagttattgt ttttagaata
     5101 caggaagctg ataaaataca tacatctcca tcatcaagtc ggaacagtgt aacctctcga
     5161 ccttcatctg aaagtatgta tgtacgcact gttccagatt ttataatcaa aataccaata
     5221 cagtcactat caccacaatg tactggattc ccttttttgt aatgcgaggt atttgctgaa
     5281 gaaattaata attctttttg taagtcagta agtttatccc aaaaaggcaa gctatgtgta
     5341 agtatatcaa tttcattttg tgaaatcata caatatctcc tttcttaaat tgttataatc
     5401 ccatgataca agtgaataga taaattgaca agtagtttgt taagaggatt atttagtcag
     5461 atagtaatag tatgatttat gtagaaaaaa tatgataata tatattcttg ctattaatga
     5521 gaaagtaata tgttttgatg atagtaggtt tgtataacta atgcataccg tttattttta
     5581 aaagattaag ttccttatgt aaccttatag acataaaacc agcaattata gcagccatca
     5641 aatcagctat tggttgtgca tataagatac catttaaccc atagaataca ggaataatca
     5701 aaattatagg gataaaacag acaccttgtc tacacatacc taaaaatcca ccttcctttg
     5761 cttttcctag tgcgaggaat aatgtagaat acactgtata gaatccaaat agcataaatg
     5821 aaaacccatt tgccctaaga gctttactac cattttctat taataaaatg tcaccacttg
     5881 taaattgtga aattatagat tttgggaaga gtatcaatat aagaccaagc actacacaaa
     5941 atactgtagt ccataatatg gaagttttta ttgctatatg taatctatcg tagttctttg
     6001 aaccaaaatt ataaccagca attggttgga aaccttttat aaaaccaaat acaacaagac
     6061 tacctaaaga cgttattcgt gttacagcac ccattccagc tatcacagaa tcaccatact
     6121 ttgtagcctc catattagtt aatgtaatag caagactagt caataattga aatattaagg
     6181 taggtatgcc aatttttagg atttctgaaa aaatttcttt tgaaaaacag cattcgttaa
     6241 ttctaaaact aaatatactt ttcttgctta aaatatatcc tacataaaca agtgttgaag
     6301 caatctgtga gatagctgtt gcaatagaag caccccttac ccccagacca aaagtataaa
     6361 tgaatatcgg gtctaaaatc atattaagca aagcacctaa taacatagca cacatagttg
     6421 ttttagcagc tccttcactt gtgacaatat tagtcatagt gacattgaat atattaaaaa
     6481 tagatgacac gacataaatg ctagcataag atattgcata aggcataata ctttttgtag
     6541 ctcctaacaa ttttaaaatt ggattgagaa aaatcataat cacaataata actaatgtac
     6601 ccactataat actactgtaa atagatgtac ttaccacttt tcctgctttt tcattatcac
     6661 catggcctaa aagtctggca acatatgaag cagctccatt tccaaacaat aatcctaatc
     6721 ccacaacaag ctgacctaat ggaaatgcaa cggatatagc tcccatttga cttgttccaa
     6781 gtccacccac aaagtatgca tcaaccaaat tgtataatgc agttatcatc atacctatca
     6841 tggtaggtaa tcccatagat aacaatgctt ttgagattgg ggcagaccct aataattcaa
     6901 ttcttttgtt actttctttc atacttaaca ctcctcttct attgacatta agtctaaatt
     6961 ataatactat aaattatagt aagtatggaa aatagtacta taatttatag tatttgtcaa
     7021 tagtaagaaa actaaaggag atgattttta tggctacaat agatttaatt gtattgggaa
     7081 tagtaaagca acaacccaag agtgcttatg atattcaaaa ggcagtggaa tacaggaata
     7141 tatcacgttg ggttaaaata agtacaccat caatttataa aaaagtaatt caattagaac
     7201 aaaagggata tattgaaagt aaaactgtga agaatggaaa aatgccagaa aaagcagttt
     7261 attcattaac tgaattaggt gagacacatt ttttagattt aatgtttgat acatctagca
     7321 aaccaataaa tattctttta gattttaatg ctgttatagt aaatttaaat agccttacac
     7381 cagaaaaaca gaaattatgt ctcaataata ttgaagagaa tgttcaagag ttaaagaaaa
     7441 ctattgagga aaatataagt ataaagacag aagtaccaga cacagcaaaa gcagtgttgg
     7501 agcaacagtt ggtgctagca caagcattgg aaacctggat aaattcttta aaagataata
     7561 tgggaaatta gaattagaga agctaactta ataatcatta aacaaaaacc agcatattta
     7621 ataatgagat attagaaaaa ataaaacaaa tgatataaac tgaaaagtat atttaaaata
     7681 aagctcactt aattgaggga gaatgttaaa ctttt
//
LOCUS       DAYASV010000163         7651 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYASV010000163 DAYASV010000000
VERSION     DAYASV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.168"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            156..1559
                     /locus_tag="ACGI68_003363"
     CDS             156..1559
                     /locus_tag="ACGI68_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0139783.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1650..1890
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1933..2631
                     /locus_tag="ACGI68_003364"
     CDS             1933..2631
                     /locus_tag="ACGI68_003364"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFH0139784.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2654..3655
                     /gene="trpS"
                     /locus_tag="ACGI68_003365"
     CDS             2654..3655
                     /gene="trpS"
                     /locus_tag="ACGI68_003365"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFH0139785.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4307..5874)
                     /locus_tag="ACGI68_003366"
                     /pseudo
     CDS             complement(4307..5874)
                     /locus_tag="ACGI68_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5867..6392)
                     /locus_tag="ACGI68_003367"
                     /pseudo
     CDS             complement(5867..6392)
                     /locus_tag="ACGI68_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ia"
     gene            complement(6781..7527)
                     /gene="cdtR"
                     /locus_tag="ACGI68_003368"
     CDS             complement(6781..7527)
                     /gene="cdtR"
                     /locus_tag="ACGI68_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HFH0139786.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2732 a          912 c         1121 g         2886 t
ORIGIN      
        1 taagatttga tttcaaagta tttaaggttt attattaaaa ctgtttttga atatgcaaat
       61 attttgaaaa atatcagttt aataataatg aatctatttt attaattttg aaattaaatt
      121 taaaataatt aatagagagg ggaaattaaa ttaggatgga aaagaaaaaa ataggcctat
      181 ggagtttggt atttatgaat gtatcagctt tatatggtat aagatggata gctaaatcta
      241 cagcatcaag ctttggttta ggattaggtg caatacctat gtggttatta ttctcattta
      301 tatactttgt accaggagct ttaatttgtg ctgaattagc ttctacatat caaaaaggtg
      361 atggaggtct ttacgattgg gttaaggaag cctttggaga aaagtatgga tttttagttt
      421 cttggttaaa ctggacagct aaaatatttt ggtattcttc atttttaact ttcttagcag
      481 taaatgtttc ttatacaata ggaaatccag cccttgctga taataagatg tttgtactta
      541 tattatcttt aataatattc tgggcattat ctcttatagc aactaaagga ataagtttta
      601 caaaaatatt tactaataca ggtgcattag gttcaacaat accaagtgct atacttataa
      661 tattttcatt tgtagcagta tttgtgttga aaaaacatga tatagcttct acatacacaa
      721 tagcttcaat gactccaaaa cttaatatgg atgcatttgt tgctatatca gctataatgt
      781 ttggtttagc aggagcagag actgctgcta acttcataac agaaatagat aagcctgaaa
      841 agaacttccc taaggcaata ttaatatcag ctggaatagt agcttcatta tatgtattag
      901 gttcaattgc aataacaatg ataataccac cagaccaaat tactgcttct aaaggtgtat
      961 tagatgcact ttctgctgtt tgtgcgtctt taggaattgg ttcttggttg gttcaattaa
     1021 tagcttttgg tatagctttt tcagtattag gagcaatagt tctttacata gcatcaccaa
     1081 taaaaatgtt atttggtagt gttaaaaaag gaatatttcc agatagttta gtagaggtaa
     1141 atgaacacaa gataccttct aaagcagtta tacttcaagc aataatagtt actataatac
     1201 tattggtgac tacattaatg ccttctgttg atgctatata caatgttctt gtaactatga
     1261 cagcattaac tgcattattc ccatatgtat tgttatatgc ttcttatatt aaactaagaa
     1321 aagagagacc agatgaaatt agaccttata ctatggcaaa atcaacaagt acttgcctag
     1381 gtttagcaaa aatggtttta gttgtaactg tagtgggtat attattatct gcagcaccag
     1441 ttatgcctac atttgctgaa aatgttatat atgagataga gatgattggt ggaggtcttc
     1501 tagttatatt atctggtcta tggttatgga atagatatga aaagaaaaca gctaaataaa
     1561 atgtatttta aaaatttctt ttggatatta taatatatag ttgacaattt atagtgtaag
     1621 gaatattata aaataaagaa attaataaat taaatcctga gataagaaat agtaaataaa
     1681 tatatttttg cacagagaat taaggatgct gagaacttaa taaaaagtat ttattgaatg
     1741 ggtctttgag gagtaaaatg aaatgtaatt taatattaca gagtatttga tatcgtgtat
     1801 gcacgttata gcatatatta agatatatat attcaatttt gagtgtatat aaatttaggt
     1861 ggtaccacgg aaaatatatc cgtcctattg atttaggacg gattttttaa tatagaaaat
     1921 ttaggaggaa aaatgaacgt aataggaatt ataggtgcaa tggatgaaga agttagtatt
     1981 ttagttgatt taatggatat tagagagact ataaaaaaag ctagcttaga attttataag
     2041 ggaattttgg aaggtaagaa tgtagtgtta gtaaagtgtg gcataggtaa ggttaactct
     2101 gctctttgtg cccagatact aataagcgaa tttaaggttg atgctattgt taatacaggt
     2161 gttgcaggag ctttaaacga aaaacttgat gttaatgata tagtaatatc aactgatgct
     2221 atacagtatg atgtagacac tacagcattt ggagacccta agggagttat accaagaatg
     2281 aagacttctg tatttaaagc agatgaaaga cttatagatg ctgcttacaa atcttctgtg
     2341 gaagaagtaa agactcataa agtattaaaa ggaagagtag ttacaggaga taaatttata
     2401 aattctaaag aattaaaaga agaattagta aatgattttg gtggatattg tggagaaatg
     2461 gaaggtggag ctatagctca tgtatgctat ttaaataata ctccatttgt aataataaga
     2521 gcaatgtctg ataaggcaga tggaagtgct gatgttactt atgatgtatt tgtacatgat
     2581 gctgccaata actctaaaga catagtttta aatatgttaa aatcaatata aatatataag
     2641 gaggtacaaa aaaatgagtg aaaaaaaagt tatatttagt ggtgcacagc catcaggcaa
     2701 aatgacttta ggaaattatt taggagctat aaaaaactgg actgaacttc aagacaatta
     2761 tgattgttat tatagtatag ttgatttgca tgcaataaca gtacctcaag accctaaagt
     2821 tttaagagcc aatactatag aattacttgc tcaatatata gcttgtggat tagacccaga
     2881 aaaaaatact atatttatac aatcacatgt caaagagcat gttgaactta tgtgggtatt
     2941 gaatactatg acttatatgg gtgaattaag tagaatgact caattcaaag ataaatcaca
     3001 aaagagtgaa gcaaatctaa atgcaggttt attcacatat ccagttctta tggctgctga
     3061 catattgttg tatcaaactg acctagttcc agttggagat gaccaaaaac aacatttaga
     3121 attagctcgt gatttagcaa atagatttaa taatagattt tcacctactt ttgtagttcc
     3181 agaaggatac tacccaaaag gtggagcaag agtgatgagt ttgcaagagc caactaaaaa
     3241 aatgagtaaa tccgattcaa atgaaaatgc atttatacta ttagcagatg attcagattc
     3301 tataaagaga aagataaaaa gaagtgttac tgattcttta ggtgttgtaa aatacaatga
     3361 tgaacaacca ggtattaaaa atctaattga tatatattct aatttaagta aaaagagtgt
     3421 agaagagatt gtaaacatgt atgaaggaaa gggttatggc atatttaaag aagatgtggc
     3481 tgaagtagta tcagaagctt taagacctat aagagaaaaa tatgttgacc ttcttaataa
     3541 taaagattat ttagaaaaaa tatatagtat gggagcagaa aaagccgaaa aacaagctag
     3601 aaaaactctt agaaaagttt ataaaaaagt tggatttata gagagaagat actaattatt
     3661 agctatcttt acttgataaa taaattttaa agttattaaa aaggtatatg atatgaaaga
     3721 gagagtctca aagtgaactt ttgttcatga ggcactcttt tttgtatgaa ataaaatata
     3781 ttttaatcat ttcaacaatg aaaaagagga ttatctctat tttgagacag catcttttga
     3841 taataaaaaa agatgagctt ttgctcatct caaaggggtt agattattct tactttgggg
     3901 aaagtagaat aaaggtaaaa gttaactgat gtcaactttg tcttttttgc atatcactac
     3961 tgagcataaa aaaatcagaa aaccatagcc ttctgaaaca acttctaaca tacattgtat
     4021 tttataccca atatgtaatt tatgagttaa ttttaactct tataatgaaa attgtcaata
     4081 atatatcaaa aaaatatttt ttaaatttat ataattaaat gaatttatgt ttatatatag
     4141 gtgatatgat acagataatt atctttttaa tacaatatag ttctaaaaaa gaatatatta
     4201 gaagaagata atatttaaat aaaaaatacc ttataaagtt aattttttaa tactttataa
     4261 ggtaaataca tattgtattg tggggataaa tttaaatcat ttgatactaa tcaacactaa
     4321 gaaccaataa ctctctatca tcttgagtaa ttgtatatat tcttaatttc ttgtatgtca
     4381 taatattttc tccacctgta aaataactcc taagattaac acaatcagtt ttaggttgat
     4441 ttgtatctcc taaataatta cgcaattctt tatctttagt ttcaatatct ttaaatccaa
     4501 aatattgtaa agtagcttca actctatatt tttggacata atcgagagct tctttattag
     4561 tttatgttgg tgtaaaatac ataccattta tataagcctt tattgtactt ggattaaatt
     4621 taaaatctgc agaatatatt ttatgtgtaa ctattatttc ttgaacagtt ggaattttaa
     4681 cttctggttc atcaagtatt tcaggagtac tatttagctc tgtaatagat aagttatcta
     4741 agtatattgt atcactatca attaatgcta tatttatatt agaagaatct ttttcagtag
     4801 tttcaaattc ataactaaat tttgtatatc cttgttctag taccaaataa tccgtttttt
     4861 cttcttttac ttttatattt acaattattg aattatatgt taaaggattt tttgaatttt
     4921 actaaaaaca taacgtttat aaggttttag ttcagacata ggtattttaa tcttcgtctc
     4981 accatttaat ctatttgctg agccttttaa accatcttga tttgtagtat taacattact
     5041 ccatatacta gggtaattat tgtaatcatc aaaattagta aagtaagttg gtgtttttat
     5101 aagtatattc attcctcttt caagtttaac attacatatt tgtttatcag acaaagtttt
     5161 taaactgtct ttaatcttat tgcctatatt atcatcaaat atgagttcaa cacaactttc
     5221 atctattggt atattattaa aatataacaa accatctttt ttagtagtgc caaaagtttt
     5281 ttcaattgct tctccaattg taagttcagg tgttttatct tctgggacct gtaaactttt
     5341 agcagtaatt cttcttttgt aagattcatt ttctgtatct aatataatag atacagaaat
     5401 actgtcaatt tgacctatat cgtcttacca actatttcat tctgttacta tttgtccaga
     5461 accatttttt gtaccaaaat ttccacttac ttgtgttgtt tctaatttaa tttgctttcc
     5521 atcatctaat ttttttaatt aattataatt tataggaatc agtctagaac taaattgata
     5581 cattgtgtta agagccatag gtgaaaatcc ttttttgaga taagtatttc caggagatag
     5641 attattgcca atttgatttt cttgtgcttt gatagttgat aatgcatctc catctaacac
     5701 taaatttgtt gttggtgtca ctttgtacat aggtgcagta cctgtgttgt agtatctaat
     5761 atttgcattt atatatgcag attctccttt gtttatactt aatccagtat ttcatgatta
     5821 tccattgcta ttttgaacag cagttgaatt atctgttgta tggtaataat ttgtcttgta
     5881 tagtttcttt aaactcatta aatttttcta atgaaatttc gttttgattt tttgttctta
     5941 ttactttatt aaatgcaaat ttttctggag attcaaaata atatacatac ataggtttat
     6001 ctattttatt ttttgatata gcatttcgaa gttctctata ttctttttct caagaatttg
     6061 cttctatttg ataatcataa aaataatttc ttgtctgaga atatttgctt atctctatag
     6121 aatctttttt atatgatcct aatacttctt tttcagattt ttcaagtttt tgatcttttc
     6181 tttctcattc tttagccttt tctttatctt taaaaaaatc cttctgatct ttctatagga
     6241 gccttgtaag tagtattaca aacttttcca ctatagcttt gtaaatcttg tgcataaatg
     6301 ttattaggta acaaactacc taaagttaga tataatatca acaatataga tatacaatta
     6361 ctaatacttt tatgtttcct aaattttttc atttattctc cctacaaata ttagtttagt
     6421 taacttttga attttaattt atttagatta tataatctaa ataagaattt tttatataaa
     6481 tttcataaaa tatgtaggta tatatcataa aaatatgtat ttatatgata tgtcagtaca
     6541 aaataacaac tgaatatttt aaagagacta tttcgtactt ttatgattta atgagctatc
     6601 ttatcgaatc ataatctagg aacaattgtt ataatatcta atagtagtta tatatgatat
     6661 agtcagtatg tgttttttca caatatgatt attatataga ggattcttag ggattactct
     6721 ttttgtataa aaaagagatg gttcaaattt ttatcttgag acatctcctt tttctattta
     6781 ttatgtttta ataatattct ttaaaatatt tctatatgta ttagatatac gacatatatg
     6841 gccattactc attttaatat tatttttctt taggtcgaga gaaacaatat gatatttatt
     6901 aactacaaaa gatttatgta cacggataaa gcatgcatct aaatctggta aagactttaa
     6961 acttttatag aattcaaata ttctagaagt agtcactaat tttattttat gtggaatagt
     7021 gcttgtttca aaatatataa cttcatcaaa tgaaattttc catagtgaga aatctgaata
     7081 gatagtaaaa aaattagagc gataattttt ttctttaatg tatcttttgt gagcaatttt
     7141 aatacaagct tttattttac tttcagcaat ttccatatca tgttttagta taaagtccat
     7201 aacttctaac ctataatcga agatccctaa aatcatttct atatgatttg taagaaatat
     7261 gattggacta atataatctg attctctaat tttttgtgct atccataatc catcacataa
     7321 ttcattactt atcttatatt ttgcgtctac aactataaaa tatattctta ctttattttt
     7381 ttcagtaagc tcttttaata acaaatttgc attatgatat aatcttacaa catcattatc
     7441 aaaaccttct tttattaaaa tattatttat tttttctttt agtttcattc caaaacaaac
     7501 atcattatca aagattaata tatccactaa ataccctcct ataaaaaatt caaaagtaaa
     7561 aaaacaaaag tattttatag aaaatacata aaataacact aggagataaa taagattaag
     7621 gatagttaga attaaggtat atttatgctt g
//
LOCUS       DAYASV010000164         7606 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYASV010000164 DAYASV010000000
VERSION     DAYASV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.169"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            18..92
                     /locus_tag="ACGI68_003369"
     tRNA            18..92
                     /locus_tag="ACGI68_003369"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50..52,aa:Gly,seq:gcc)
     gene            117..193
                     /locus_tag="ACGI68_003370"
     tRNA            117..193
                     /locus_tag="ACGI68_003370"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:151..153,aa:Arg,seq:tct)
     gene            203..278
                     /locus_tag="ACGI68_003371"
     tRNA            203..278
                     /locus_tag="ACGI68_003371"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:236..238,aa:Gln,seq:ttg)
     gene            287..362
                     /locus_tag="ACGI68_003372"
     tRNA            287..362
                     /locus_tag="ACGI68_003372"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Lys,seq:ttt)
     gene            365..453
                     /locus_tag="ACGI68_003373"
     tRNA            365..453
                     /locus_tag="ACGI68_003373"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Ser,seq:tga)
     gene            457..532
                     /locus_tag="ACGI68_003374"
     tRNA            457..532
                     /locus_tag="ACGI68_003374"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:490..492,aa:Phe,seq:gaa)
     gene            539..612
                     /locus_tag="ACGI68_003375"
     tRNA            539..612
                     /locus_tag="ACGI68_003375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:573..575,aa:Met,seq:cat)
     gene            627..703
                     /locus_tag="ACGI68_003376"
     tRNA            627..703
                     /locus_tag="ACGI68_003376"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Ile,seq:cat)
     gene            721..797
                     /locus_tag="ACGI68_003377"
     tRNA            721..797
                     /locus_tag="ACGI68_003377"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:755..757,aa:His,seq:gtg)
     gene            806..881
                     /locus_tag="ACGI68_003378"
     tRNA            806..881
                     /locus_tag="ACGI68_003378"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:839..841,aa:Lys,seq:ttt)
     gene            889..962
                     /locus_tag="ACGI68_003379"
     tRNA            889..962
                     /locus_tag="ACGI68_003379"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Cys,seq:gca)
     gene            970..1046
                     /locus_tag="ACGI68_003380"
     tRNA            970..1046
                     /locus_tag="ACGI68_003380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1004..1006,aa:Arg,seq:acg)
     gene            1059..1134
                     /locus_tag="ACGI68_003381"
     tRNA            1059..1134
                     /locus_tag="ACGI68_003381"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1092..1094,aa:Val,seq:tac)
     gene            1210..1623
                     /locus_tag="ACGI68_003382"
     CDS             1210..1623
                     /locus_tag="ACGI68_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139787.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            1932..5363
                     /locus_tag="ACGI68_003383"
     CDS             1932..5363
                     /locus_tag="ACGI68_003383"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFH0139788.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTRESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5605..7533
                     /locus_tag="ACGI68_003384"
     CDS             5605..7533
                     /locus_tag="ACGI68_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFH0139789.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3021 a          935 c         1515 g         2135 t
ORIGIN      
        1 tttaatttaa taaacgagcg ggaatagttc agtggtagag cgcaaccttg ccaaggttga
       61 agtcgcgagt tcgaatctcg tttcccgctc caaattttat gtttatgttg taatgtgggt
      121 gcatagctca gctggataga gcaacgccct tctaaggcgt gtgtccgggg ttcgaatccc
      181 tgtgcgctca ccacatatat attggggatt agccaagtcg gtaaggcaca tgactttgac
      241 tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa cagtatggtc tattagctca
      301 gtcggtagag cacctgactt ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac
      361 caatggagag gtgtccgagt ggtttaagga gctggtcttg aaaaccagtg acttcgaaag
      421 gggccgtggg ttcgaatccc accctctccg ccaaatgccc agatagctca gtcggtagag
      481 caggggactg aaaatccccg tgtcggtggt tcgattccgc ctctgggcac caaaatgtgg
      541 cggtatagct tagttggcta gagcgttcgg ttcatacccg aaaggtcata ggttcgactc
      601 ctattaccgc tactgaaatt taatgtgggc cattagctca gttggttaga gcgcccggct
      661 cataaccggt aggtctgggg ttcgagtccc tgatggccca ccacattaaa ttaacttatg
      721 gtgggtatag ctcagttggt tagagcgcca gattgtggct ctggaggtcg tgagttcgac
      781 tctcattatc caccccaact tatatggtct attagctcag tcggtagagc acctgacttt
      841 taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc aaaaaggcgg cgacatagcc
      901 aagtggtaag gcagtggact gcaactcctt gatccccagt tcgaatctgg gtgtcgcctc
      961 caataaaatg cgctcatagc tcaactggat agagtgtctg actacgaatc agaaggttag
     1021 gggttcgagt ccctttgggc gcaccatatg aatagcgagg gattatagct cagctgggag
     1081 agcacctgcc ttacaagcag ggggtcacag gttcgagccc tgttaatccc accagtgcaa
     1141 acctctagta aaactactag aggttttatt tttgattgaa aaagttttta tatttaggag
     1201 gtaaaaaatt tgtatatagt aataggaaat gaaatagttg atagtgaaga acttaaaata
     1261 attatagata aaaatagtga atttaaagtt gaaaaagatt taagtaaaag cacaaaaaga
     1321 gaagatgtaa tagcatatca gttaagtata gatttaaatt acctagattc tcttataaat
     1381 gagcaatgta atttagccag tctatctgat gaagagaaat ttgatgaata tatgactttg
     1441 tctgatgaac ttgcactgga tttagaagag ttaatgccaa aatatacaat aataaatgct
     1501 agagcatata aattagacga ggtagatgga attgtaaaaa taatactagc tgtagcgtat
     1561 gcagatttag ggcacttgaa actttctgat gtagtaaaga ggctatctag acaagtagat
     1621 taggatatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
     1681 aattatgtaa acatagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat
     1741 aaattcatct gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca
     1801 cagtctaaaa attacttata tttataaatt tatcttatta attaaggaac tttcttaaac
     1861 atcttcaaaa ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag
     1921 aagaatagag tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag
     1981 ctataagaat atttagagcc tgttcagaat tggggataaa aagtgttggt atatacagta
     2041 aggaagataa gtatggattg tttagaacaa aagcggatga atcatatttg ataggagaag
     2101 gtaaagggcc aatagatgca tatttagata tggatggaat aatagaccta gcaaagagaa
     2161 aaaaagttga tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc
     2221 gtaaatgtga agagaatgga attactttta taggaccatc atctaaggta atgaatatga
     2281 tgggggataa gattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag
     2341 gagtagaaaa agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggat
     2401 atcctgttat gataaaagca tctaatggcg gtggcggtag aggtatgaga attgtacatc
     2461 gcgaagaaga cttagagctt gaatatgaaa ctgcttgtag tgaatcaaga aaagcttttg
     2521 gagaagatat tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa
     2581 tattaggtga taattatgga aatatagttc acttatatga aagagattgt tcagttcaaa
     2641 gaagacatca aaaaataata gaatatgcac cagctttttc attagatgat aaagtaagaa
     2701 aagagatatg tgaagatgca gttaagttat caaaacatgt gggttattct aatgcgggaa
     2761 cattagaatt tttagtagat gcaaatggtg ggcattattt tatagagatg aacacaagag
     2821 tacaggttga acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa
     2881 tactaatagc ccaaggttac agcttagata gtgaagaaat taatataaaa tcacaagatg
     2941 atgtggaaat aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata
     3001 aattcatgcc tgatacaggt aaaattcaag tttatagaac tggttcagga tttggtataa
     3061 gactagatgg aggaaatgga tttacagggg caaacataag tcctcattat gatagtttat
     3121 tggttaagac aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat
     3181 ctataaaaga acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt
     3241 taaataatcc tatattctca aatggtaaat gtagtactaa gtttatagat gaaaatccag
     3301 atttatttga aataacagaa agtaaagaca gaggaacaaa attacttcaa tttataggag
     3361 atgtaattgt aaatgataat gcttgtaaag aaaaaccatt atttgatgca ttacatgacc
     3421 caagaatgga taaggatggt tcaaaatcag aaggaagtaa aatacttttc gataagttag
     3481 gtaaatctgc atatatagaa aagataaaaa atgataagaa attactatta acagatacaa
     3541 caatgagaga tgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa
     3601 aagctgctaa gccaacagaa aaatatcaaa aggatttatt ctcacttgaa atgtggggag
     3661 gagctactta tgatgttgca tatagatttt taaaagaatc tccttggcgt agattacaaa
     3721 agctaagaga agaaatacca agcataatgt tccaaatgtt gcttagagca tcaaatggag
     3781 ttggatataa aaactatcca gataatgtaa tagaagaatt tacaagagaa tctgctagac
     3841 aaggtataga tgtatttaga atatttgatt cattaaactg ggttgaaaat atgaaaccat
     3901 ctattaatac agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg
     3961 atatactaga taagactaaa actaaataca atctagaata ttatattaaa atggcacaag
     4021 aattagaaag cttgggagca gatataatag caataaaaga tatgtcaggt cttcttaaac
     4081 catattcagc ttacacatta gttaaagagt taaagaaaaa tgtaaaagca ccaatacatt
     4141 tacacactca tgatacaagt ggaaatggtg tagcaacttg tttaatggcg tcagaagctg
     4201 gagtagacat aattgatgct gcattagaat ctatggcagg acttacaagt cagccatcac
     4261 ttaatgctat agtagaggca cttaagaata cagagagaga tacaggaata gatttatttg
     4321 gatatgatga gttaggaaaa tattataagg atttaagaaa agtttataat aaatttgaaa
     4381 gtgatttaac taattcttgt gcagaaatat ataattttga aataccagga ggccaatata
     4441 caaacttaaa acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag
     4501 aaaaatataa agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa
     4561 aagttgtagg agacttagct atctttatga ctaaaaataa attagataaa gacaatataa
     4621 tagaagaagg taaaaattta tcattcccag attctgttgt ggattactgt aaaggtatga
     4681 ttggtcaacc agaaggtgga ataccaaaag atttacaaga ggtagttcta aaaggtgaag
     4741 aagctataac agtaagacca ggttcattat taccagcaga agattttgat gaaatagcta
     4801 aatatctaaa tgaaaagtat gatataaatg ctaatataag aaatgttata agttatgctt
     4861 tatatccaaa agtatatgaa gattatatca aacatctaca acattataat gatatatcta
     4921 aacttgaaag tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtagaaa
     4981 tagaagaagg taaggtatta acaataagat tagttgaaat tggtgaagtt aaggaaaatg
     5041 gatttagaac tataggattt gagttaaatg gaatggttag agaagtagaa ataaaagata
     5101 agaatttctc tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa
     5161 ttggagcaag cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa
     5221 aagcaaatca accattaatc gttatagagg ctatgaagat ggaaactata atagttgcta
     5281 agactgatgg agtaattaag tcaatcaaag taaaagaaga tgatatggtt gaagataaac
     5341 aattattaat gataatgaag taatttaatt aaaataaatt aaaaagcact ctgaaatgtt
     5401 taataaaaaa tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag
     5461 aggacaatta cctccagatg tagaaataaa gctagtaagg aaaatgttat ctaaaaattc
     5521 taaatatagt aaatattttc cataatattt cttaggaaat aacagcaaag ataaaataca
     5581 taaatttaaa aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta
     5641 gcggtattag gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca
     5701 gcaaacacta ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga
     5761 ttaataccta tagagtataa tgattataag tttaacttat tggatacccc aggatatttt
     5821 gactttagcg gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata
     5881 gatgcaacag cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt
     5941 cctaagataa tgtttataaa taaaatagat aatgaaaagg caagatacaa agatgcaata
     6001 gcaatgttaa gagaaaaata caataataag atagttccta tgataagtcc tatatacaaa
     6061 gataaaaact ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa
     6121 tttaaggaac aagctatgag tgttaaagaa gcattaatgg aacttatagc agaaactgat
     6181 gaccaaatat tagataaata ttttaatgga gaagaactaa ctacagaaga gatacaaaaa
     6241 ggtattatta taggtataca aagaggtgat attatacctg taatatgtgg ttcaactata
     6301 aataatatag gcacaaagga aatattagat acaatatcaa gttatttaga acctatcttt
     6361 acagaggaaa gcaaaccatt taggggatta gtatttaaaa ctatggttga cccatttgtt
     6421 ggaaagatgt catatataaa aattactgag ggtgtacttt ctaaggataa agatgttttt
     6481 aatataaata aaaatgtaaa agaaaaaata gctaatatat atacattgag aaatagtgaa
     6541 ttagtagaaa tagaaaaagc taaagctggt gatatagtag ttataactaa agttaattct
     6601 ctaaaaacag gagacactat atctgcagat aaagatgcag aagctttaga aaagatagat
     6661 tttcctaaac cacaaatata ttatgcagta actcctaaga ataaaggtga tgaagaaaaa
     6721 gtagcatctg tattaaataa actagtagaa gaggacccta cattacattg gtatagaaat
     6781 acagagacaa aacaagcact tttaggaggt caaggagaac ttcatataaa aacaattaaa
     6841 aataaaatga aagataagtt tggtgttgat gttgaattga atgaccttaa agttccttat
     6901 agagaaacta taaaaggaac agctgatgta caaggaaaac ataaaaagca atcaggtggt
     6961 catggtcaat atggagacgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt
     7021 acagaagaaa tatttggtgg ttctgtacca aaacaatata taccagcagt tgaaaaaggt
     7081 ttaaaagact ctatgcaaaa aggtatatta gcaggatatc ctgttacaaa cataaaagct
     7141 acgctttatg atggttcata ccatgatgta gactcttctg aaatggcctt taaaatggca
     7201 gcaagtgcag cctttaaaaa aggtatggaa gaagcacacc caatactact agaacctata
     7261 atgaaactta agataactgt tccagaagaa tatatgggag atgtcatggg tgatataaat
     7321 aagagaagag gaaaaatatt tggaatggaa ccagatgaca aagggaaaca aataatattt
     7381 gcagaagctc cacaagcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa
     7441 ggtagaggat actttgaaat ggaattagag agatatggag aggttccatc acagtttgca
     7501 gaaaaaataa ttgggttagc tactgcaaag taatgaacaa aataaataga aaaataatta
     7561 atatatatta ttaaaaatat ttttagggtt ttaactaggt ataggt
//
LOCUS       DAYASV010000165         7546 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYASV010000165 DAYASV010000000
VERSION     DAYASV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.170"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            124..633
                     /locus_tag="ACGI68_003385"
     CDS             124..633
                     /locus_tag="ACGI68_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFH0139790.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            645..1526
                     /locus_tag="ACGI68_003386"
     CDS             645..1526
                     /locus_tag="ACGI68_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFH0139791.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     misc_binding    1654..1859
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1985..3256
                     /gene="serS"
                     /locus_tag="ACGI68_003387"
     CDS             1985..3256
                     /gene="serS"
                     /locus_tag="ACGI68_003387"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFH0139792.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     gene            3577..4032
                     /locus_tag="ACGI68_003388"
     CDS             3577..4032
                     /locus_tag="ACGI68_003388"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HFH0139793.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            4033..4124
                     /locus_tag="ACGI68_003389"
     tRNA            4033..4124
                     /locus_tag="ACGI68_003389"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4067..4069,aa:Ser,seq:gga)
     gene            4162..4426
                     /gene="ffs"
                     /locus_tag="ACGI68_003390"
     ncRNA           4162..4426
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGI68_003390"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            4503..6140
                     /gene="dnaX"
                     /locus_tag="ACGI68_003391"
     CDS             4503..6140
                     /gene="dnaX"
                     /locus_tag="ACGI68_003391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFH0139794.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            6199..6549
                     /locus_tag="ACGI68_003392"
     CDS             6199..6549
                     /locus_tag="ACGI68_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFH0139795.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            6562..7161
                     /gene="recR"
                     /locus_tag="ACGI68_003393"
     CDS             6562..7161
                     /gene="recR"
                     /locus_tag="ACGI68_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFH0139796.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            7302..7523
                     /locus_tag="ACGI68_003394"
     CDS             7302..7523
                     /locus_tag="ACGI68_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139797.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
BASE COUNT         3077 a          755 c         1390 g         2324 t
ORIGIN      
        1 tcttttttat ttattataaa agttaagatt aaatagaata taaagtcaat attgatttat
       61 aataagataa aatataaaat tatatagtat aggtaaagtt tttatttgga aagaaggtgt
      121 aatatgaatt ctaatgaaag aagaagtaaa ttaatagata ttttaaaaga atctaagcat
      181 cctgtaaaag gtggaacact agctgaacta ttaaatgtaa gtaggcaagt aatagttcaa
      241 gatatagccc ttataagagc tagaggattt gaaataatag ctacacctca aggatatata
      301 atatataatc aaccgtattt tacaaaacaa ataaagtgta aaaatcataa ggattctaaa
      361 gaaatttatg atgaattaaa gataattgtg gatttaggtg gtattataaa agatgtcatc
      421 gtaaatcatc caacatatgg tcagataact gcagaactta atatttattc taaaatggat
      481 atagacaact ttatgaagaa agttgagaca aatgaattta aacaattatc ggttctgaca
      541 gaatgtagcc atattcatac aattgaggca ataaaagaag aaactataga agctatagta
      601 aaagagttaa aggacaacgg aatattatca taaggaggag ttgaatgaat tttttatcat
      661 tggctaagac tgcctcaaca aatagcaata taactaagag aactttagaa aatatgctcg
      721 ataattttag tgataatata ccaaatatta tatatgcaat aattgtattt gtgataggta
      781 tttatatatc taaaatagta agaaggatgg tttcaaagtt tctaatcaaa tatggaatga
      841 gtaaaggtgt aaataatttt attgtatatg gaatatatat atctatgtta tctattatat
      901 cattgataag cttaggtatt atagggatac aaactacatc agttgtagct gtgttaggag
      961 ctgctggatt tagtataggt ttagctttta aagaaatatt atcaaattta ggctctggta
     1021 tgattatttt attttttaag ccatttaata taggtgatta tattcaaggt tcaggtgtag
     1081 aaggtacagt ttctgacata caaatattta gcactgttct taagacgcca gataataaaa
     1141 ctataattat acctaatttt caactgacaa gtaataatat aattaattac acacatcaaa
     1201 acaaaagaag aatagatttt tcatataata tttcatatga tagtgatata gatgtagtta
     1261 agtctatatt aaatgaaatt tttacaaacg aaaaaagaat attaaatgat ccaaagccta
     1321 taataggttt aaactctata ggaaataaca ccatgcaaat agttgctaga ccttgggtaa
     1381 aaactgatga ttactgggat gtatattttg atgtaatgga aaaggtaaaa aataaatttg
     1441 atgagaatga tataaaagtt ccatttgttc cttcttcatt attatttaat aatacaggta
     1501 atctaaatac aatggataaa agataaggtt ttaattaaat agaaaaaatg aatttaatat
     1561 aaaaaattaa agaaaattct aaaaaaattt gaataatagt attgaaagat gttaatatat
     1621 ataatataat gattatcaaa taaaacaatt agttaaaaag ctgtgaagga aaatagtaaa
     1681 aaacagattg tattcagaga atgagtggat ggtgaaaact cattacaatg gtttttgaat
     1741 ctactctaga gcttctagtt aaagctagac gtttacctac gttataggtc tgagaggatt
     1801 atacatttta gtatagtcaa ttagggtggc aacgcggata aaagattttc gtccctttta
     1861 tagggtaaac ggaggtcttt ttttattttt aaaatttgtt taaactaaga gtttacaagt
     1921 ataatgtttg taaattaagt tggaattata acttatatta tcaaaattat taggaggaat
     1981 taatatgtta gatattaaaa gaataagaga aaatctagat gatatcaaaa aagctatgga
     2041 aagaagaggt gaaagagaat ttgaccttga tgctgtagtg gaattagata ataaaagaag
     2101 agaaatactt caagaagttg aagttatgaa aaatgagtta aatgtaaatt ctaaaaaaat
     2161 acctcaatta ataaaagaag gaaaagatgt aacagaagaa aaggcaagat tgaaagagtt
     2221 atcagataag ataaaaggta ttgatgagaa agtaaaagaa gtagaagcaa aaatggaata
     2281 tacattaatg agaataccaa atgttcctca tccagaggta ccacaaggtg aaacagatga
     2341 agacaatgtt caaataagaa cttggggaga acctactaaa tttgattttg agcataaagc
     2401 gcattgggat ataggtacag gtctaggtat tttagatttt gaaagagcag ggaaaataac
     2461 tgggtcaagg tttactttat ataggggatt aggagctaga ttagaaagat ctttaatgaa
     2521 tttcttttta aatactcata cagctaagca tggctatacg gaagtcttac ctccatttat
     2581 ggccaataga aatagtttta tagggactgg tcaacttcct aaatttgaag aagatatgtt
     2641 taaaatagaa ggattagagt atttcttaat accaacagct gaagttcctg ttactaatat
     2701 acatgctaat gaaatattag atgttgcaga gcttccaatt aaatactgtg catatactcc
     2761 ttgttttaga tctgaagcag gttcagcagg aagagataca agaggtttag ttagacaaca
     2821 tcaatttaat aaagttgaat tagttaaatt tgttaaacca gaagattctt ataatgaatt
     2881 agaatcatta actcatgatg ctgaaactat gttacaaatg ttaggtcttc catatagagt
     2941 tgtaaaaata tgtacaggag atttgggatt tacagctgca tttaaatatg atttagaagt
     3001 ttggatgcca agttacaata gatatgtaga aatatcttct tgttcaaatt ttgaagattt
     3061 ccaagcaaga agagcaggaa taagatttaa aagagataaa aaatcaaaag cagaatatgt
     3121 tcatacatta aatggttctg gattagcaat aggaagatgt ttggctgcta tattagaaaa
     3181 ttatcagcag gcagatggtt ctgtagtagt tccagaagaa ttaagacctt atatgggtgt
     3241 tgatgttata aaataaattt atattaattg tatatataga gtatgctttg atgaagagaa
     3301 tatattatga gtaaacaata aataaaaaaa ctatgaaatt attataaata ttaatttcat
     3361 agttttttta tgttaattta cacagcagaa ttttcatcat aaaacactaa atatttttat
     3421 aaaacactct tgtatgaata tctaaaaatt attttagaac taattttaaa aaaatatagc
     3481 tatatatagt aataattaaa taaacaaaat aaaattaagt tgaattataa aatgaattat
     3541 gataagataa gtttgttata agttttggga ggaattatgg aaagttcatt ttatatgaaa
     3601 gaagcattaa aagaagcata taaagcatat aataaaaaag aaacaccaat aggtgctatt
     3661 atagttaaag ataatcaaat aatagctaga gctcataacc ttacagaaac gttaaaagac
     3721 tctacggcac atgcagaaat tttagcgata aaacaagcat cagaaaaatt aggtggttgg
     3781 aggttgactg attgtgattt atatgttaca atggaacctt gcataatgtg tagtggcgca
     3841 atagttaatt ctagaataaa aaagcttata atagggacaa gacatgtaaa aaattcatat
     3901 atagaaaaac agcatgagtt taaattagat tattttaata ataataatgt aaaagtagct
     3961 tttgatgttt tgcaagaaga atgctcgatc atattgcaag aattttttaa ggcattgaga
     4021 aaaagagatt aaggagagat ggtcgagtgg tcgaaggcgc tcgcctggaa agcgagtata
     4081 tgggtaaaac tgtatcgtgg gttcaaatcc cactctctcc gccagaaaca atgaaagtaa
     4141 ttaaatatgg ttgtaatttt gctgtactag atggggaggt agcggtgccc tgtaacctgc
     4201 aatccgctat agcaggattg aattccatct aaaggcttat tttctgtagg gccgccctaa
     4261 ataagtggtg ttgatgctta ggtcctgcgc aatgaaagct catgaacccc gtcagatccg
     4321 gaaggaagca gcggtaagtg atcacttttg tgtgccgtgg gggtgcctaa gttgagctaa
     4381 ctgttttggt aacgcctatg gaattaagtc gatagatggt gtacagcatt taatatataa
     4441 aagactaatc ccttagataa tgagggattt ttttattaat tttgctaaag agaggtaaaa
     4501 agatgcataa agcattatat agagcatata gaccacaaaa atttgaagat gttattggtc
     4561 aagatcatat tattaagact ttaaagaatc aaatatatag tgataatata ggacatgctt
     4621 atttgttttg tggaacaaga ggtactggta aaacatctac tgcaaagatt ttttcaagag
     4681 cagtaaactg tttgaataaa ataaatgaag aaccgtgtaa tgagtgtgag atatgcgaat
     4741 ctgtattaaa agataataca atggatgttg tagaaataga tgcagcttca aataatagtg
     4801 tagatgatat aagagagctt agagaaagtg tgaaatattc tccagccaat gctaaatata
     4861 aagtatatat aatagatgaa gttcatatgt tgtctcaagg agcttttaat gcattattaa
     4921 agaccttaga ggaacctcca tcatatgtaa tttttatatt agcaactaca gagcctcaca
     4981 aaatacctgc aaccatttta tcaagatgcc aaagatacga ttttaaaaga gttacagtta
     5041 aagatatgac ccttagaatg aaaaagattt gtgaagatga aggaattgat atagatgata
     5101 aagctcttaa tttaatagct agaaattctc aaggagctct tagagatgca cttagtatat
     5161 tagaccaatg tatgtctttt ggagaaagta agatagatta taaagatgtt gttgaattaa
     5221 tgggaagcgt aaatatagaa caattatttg aattgtctca atgtatagta gagcaggaca
     5281 ctaaaaagtc tcttgagata ttaaatgaat ttgtcttatg gggcaaagat ataagaaatc
     5341 ttataaatga cttaattgat cattttagaa atttaatggt atgtaaggtt tcatcagaat
     5401 tggatgaaat aatttcatta ccagaagaaa ctatagaaca acttaaaatt cagtctaaaa
     5461 atatagatat aaatgattta ataaggattt taaatatatt atcaataact caggatgata
     5521 taaagtcatc ttcaaatcca agggttttag tggaaataac cataatgaaa attgctcaac
     5581 caatgtttga tgaaagtaaa gaagctctta taaaaagagt tgagaactta gaaaaaatga
     5641 ttgaactggg gaactttaag tctgaaaaaa ttgggaataa taaagaaaaa gagtatgagg
     5701 tcgatagaga aatagatgta aaacaagaaa atgtagttta tgaagatgta aaaaatgagg
     5761 acgttatact tatagagtca tcttggaaga atatacttaa acaaattaaa aaagataaaa
     5821 aaatgccaat atacgcactc ttaagtgaag taaaaagttt taatgtatat tctaatatgt
     5881 tgtatgttat atttgatgat aaatttgatt ttgcaaaaac tagactgagc tctcaagata
     5941 caataaatta tttagaaaaa acaataagag atgttttaaa tagaagtttt aatgtaaaaa
     6001 tagtactgac atcagaagtt aaagatataa atcttgaagt taaagagaaa aaggacattg
     6061 gtgaggaaat attaaagaac atagtgagtg aagagatatt agaaataaaa gatagtattg
     6121 atgaaaatga gagtaaataa taaaatacta ttatgatata ataataaaat gaaaataagt
     6181 ttaggaggaa tagtgtttat ggctaaaaaa ggttttggag gcggaatgat gcctggtggt
     6241 ggaaatatga acaacttgtt aaagcaggct caaaaaatgc aagaaaatat gcaaaaagct
     6301 caacaagagt tagaaagtaa agaagtagaa gcttcagttg gtggaggagc agttactgta
     6361 aaagttaatg gtaaaaaaga agttatagat ataactatca agccagaagt agttgatcca
     6421 gatgatatag aaatgctaca agatttagtt ttaagtgcag taaatcaagc attgagaaat
     6481 attgatgata tacaagcaag tcaaatgagt aaggtaactg gaggtatgaa tatacctggg
     6541 ttattctagt aggtggtatt tatgcaagtt tatacaggtc ccataacgag acttatagag
     6601 gaattttcaa agcttcctgg agtaggaaga aagactgctc aaagattggc ttttcatatt
     6661 attaatatga atactaatga tgtggaagct ttatctaaag cgattataga tgcaaaaaga
     6721 gaaataagat attgctcaat ttgttgtaat ataacagata cagacccatg tagcatgtgt
     6781 tcaaataaaa gtagagattc aagtgttata tgtgttgtgg aagatccaag agatgtagca
     6841 gctatggaga gaacgagaga gtttaaagga cagtaccatg tattaaatgg agtaatttct
     6901 ccaatggatg gaattggacc agatatgtta aaaataaagg aacttatcca aagacttggc
     6961 aatcaggaag tcaaagaaat aataatggca acaaatccta ctattgaagg tgaagctaca
     7021 gccatgtata ttgctaggtt agtaaaacca atgggaatca aagtcactcg gatagctcat
     7081 ggtcttcctg ttggaggcga tttagagtat gcagatgagg tcacaatatc aaaagcacta
     7141 gaaggcagaa gagaaatata aaaatcgagg ttaatacctc gatttttgta attttttgag
     7201 ttaaattcaa attaattttg atttagattt attgaataaa tttcttctgt attgtataat
     7261 ttgttgtata aatcatttaa aaattttgat aggggtgcaa catgaaacca attatgaact
     7321 tagaagaaca taatgcaatg aaagaaaaaa gagttaaaaa aatgaaatca aaagataaac
     7381 ctaaatatga tgattttgag gatataagtt tttctaataa gaaaaaaact aaaaaaaatg
     7441 tacgaaataa aaatgctgaa tttagaaata attataaaaa ttatcagtat gattatgaag
     7501 aagactttta tgaaccaatt taatattaaa taattagaaa ataaaa
//
LOCUS       DAYASV010000166         7358 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYASV010000166 DAYASV010000000
VERSION     DAYASV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.171"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            152..2182
                     /locus_tag="ACGI68_003395"
     CDS             152..2182
                     /locus_tag="ACGI68_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFH0139798.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKEWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKISSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            2179..2631
                     /locus_tag="ACGI68_003396"
     CDS             2179..2631
                     /locus_tag="ACGI68_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0139799.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            2633..3688
                     /locus_tag="ACGI68_003397"
     CDS             2633..3688
                     /locus_tag="ACGI68_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFH0139800.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            3755..4063
                     /locus_tag="ACGI68_003398"
     CDS             3755..4063
                     /locus_tag="ACGI68_003398"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFH0139801.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            4167..5432
                     /locus_tag="ACGI68_003399"
     CDS             4167..5432
                     /locus_tag="ACGI68_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HFH0139802.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            5520..6236
                     /locus_tag="ACGI68_003400"
     CDS             5520..6236
                     /locus_tag="ACGI68_003400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0139803.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHELEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(6431..6724)
                     /locus_tag="ACGI68_003401"
     CDS             complement(6431..6724)
                     /locus_tag="ACGI68_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HFH0139804.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(6730..6945)
                     /locus_tag="ACGI68_003402"
     CDS             complement(6730..6945)
                     /locus_tag="ACGI68_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139805.1"
                     /translation="MRILSETEKISLAAIIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
BASE COUNT         2797 a          754 c         1309 g         2498 t
ORIGIN      
        1 attttcaaat gttgtaagtt gtggctaaag ctttattgta ttattctgaa tatagaaaga
       61 tagttactgt cttagataaa aactgaaaca tacataataa tataaaattt gctatattaa
      121 taactctaat tattttggaa ggagggaaaa tatgataagt gggagaatgt tactcatcat
      181 gaattttcta aaaggaaaaa ataagacaag ttacaaggat attagcaaac aacttagtat
      241 agaagaaaga aaagttagat atgatataaa taatttaaat attgttttgc gttcattaaa
      301 caagtcagta atacaaaaga tgaacaaagg tgtgttaatt attcctgatg acttccaagt
      361 agtatgtata aataataacg aatatgtatt ttccttaact gagcgagtat ctattatgaa
      421 aattatatca atgtttaaaa tagataatct aaacttagaa aaattaagta aagaatttca
      481 ggtttctaga agttcaatta aaaatgacct taatgttttg tcaagtgaat tagaaagaaa
      541 ccaaataact attacttatg ataaatcttt taaaataagt ggagaagaag acattatata
      601 taagcaaaga ttgaacatat taaaatcgta ttcttatctt tttggtaagg aaaaatctga
      661 tttaagtgta tttgagaagt atttagtgaa tgtaattgaa aaaatattca aagggattta
      721 tttgattgat atatataatt ggtctatgaa tttattattg caaatgggat ggactttaaa
      781 tgatgaatct tttacatggt atgtgtcaaa cattttcctt tttacatggt atatacattc
      841 agatgaaaaa catcctttag aatctgcttc attaagtgag ccgtgttttg aaaataagtt
      901 tgaccaagaa tttgaaacaa ttattcaaaa aaagttgact aaggaacaaa tttttctgtt
      961 aaagagtttt gtatttttta caaataaata tgcaagttta aacgaggata tggatttgat
     1021 tagtacagaa attatagttc aagatctaat ttacaatatg tcagaaatat tagagattag
     1081 ctttaaagaa gatatgattc tttataaagg attactcaat catattgccc cattgataga
     1141 gagaatcaaa cagaatatac agatttatga ggaattactt catgtaatac cagataaata
     1201 tcaatatgta cttgaagcta ctaaggtttc tattcaaaaa aacagagttt taagtcaaat
     1261 aaagaatgaa aatgaaacaa tacttttggc aatacatttc ttaggaagta tacatagaaa
     1321 tgagaggaat aactacaaaa atatattgtt agtttgtgga tttggatatg gtgtaattgc
     1381 tatgttaaag gatagtttag taaatgatta ccagataaat atagtagaga gtattccatc
     1441 atataagtta caagattata aagaatggga aagtattgac ttcattataa caacatcaaa
     1501 gatagtaata gacttgccta aaacaattat tgagataaat ccatttttac aagaagagga
     1561 ttatttgaag tttgaagaaa aagggataaa aagaaagaac gtcttaacta attatatgtc
     1621 aataaaaaaa aggttggact ttttagaaga agatgcccaa aaaagagtat taagtattat
     1681 atgtcaagaa cttggataca gtgatgagag aatattattt agacaactta atttaagtga
     1741 tttaatagga attgatacca taaggataat tgatggagat atgaaatggg aagatgctgt
     1801 aaaaattagt tcaaacatac ttgctaattg tgattttgta tcagcacaat atgctgagaa
     1861 tattatagat attttaaaaa atgtgggttt ttatgctgta aaagataacg aatttgcttt
     1921 actacatggc aatgatagtt cattagtcaa agtaagttcg ataagtttat taatatgtaa
     1981 agaagcagtt agttttggag ataaaaaggt taaaatcata ttttgcctag caagcagaga
     2041 taaaaaagag caaattcctg caataattaa tctaactaaa atggtttata agactaattt
     2101 tattgctatg ttagaaagtg caaagacaaa agaagaagca gaagttttaa ttcataaata
     2161 tgagaaggag gtcactgtat gaataaatta gttaatgtaa aatgttcatt taaaatacat
     2221 gttaattcaa aagaagaagc cattatatcg ttagttgatg tagtgtcaaa agaaggatat
     2281 ttaatagata aaaatcaatt tttaaaagat gttttaaaga gagaagaaac attgtcgact
     2341 tatattggac atggtatagg tcttcctcat agtcaaagtg taggtgttaa aaactcttgt
     2401 attacaatag gaaagcttga tactcctatt gaatggacag aagaaggaga aaaagtggat
     2461 ttaatatttt tgatatcagt aacaaaagac aatgaaaata atcttcattt aaagatttta
     2521 tcaaaattag cacgtttatt gatgcatgaa agttttagaa atcaaattag atgttcagat
     2581 gaacaaactg tttataatct tataaaagaa aaaatagagg aggaagatta aaatgagaat
     2641 tttaaagttt ttgcaaaaac atcttatgac agcaacatct tatatgattc catttgtggt
     2701 tgcaggagga atattatttg ctttatcagt aactttaagt ggacaagctg ctgttcctga
     2761 aacaggctgg ttggctaaac tgaatcaaat tggagctgct ggcttggctt tattcattcc
     2821 tatattaggt ggatatatag catttagtat ggcagataaa ccaggattag ctccaggtat
     2881 gataggtgca tatttagcaa aagaagtaaa tgcaggtttt ataggtggta tcatagcagg
     2941 atttatagca ggatttgttg ttttacaatt aaagaaaatt aaattagctc ctacaatgag
     3001 aactttaggt tctatattta tttatccttt agttggtaca ttgattacag gtggtataat
     3061 tgtattttta attggagaac ctatagcttc ttttatgaca tggatgacaa actggttaaa
     3121 tggaatgtct ggtgtttcta aaattccttt aggtggaatc ttaggaggaa tgatagcctc
     3181 agatatgggt ggccctataa ataaggttgc tgctactttt gcccaaactc aagttgacac
     3241 acttccatat ttaatgggtg gagttggcgt tgcaatttgt atacctccaa ttggtttagg
     3301 attagctaca ttattgtttc caaaaaaatt tagcaaggaa gaaagagatt caggtaaagc
     3361 ttcactatta atgggatgtg ttggaattac agaaggagct attccatttg caacatcaga
     3421 tccagttaga gtaatacctt gtattatggc tgggtcgatt gtaggaaata ttatggcttt
     3481 tttattagga tgtttaaatc atgcaccctg gggaggtctg atagtgttac ctgtagttga
     3541 caataggttg gggtatattg catcagtatt aactggagca gtagttgttg cggtattaat
     3601 gaaattagta aaaaaagacg tgaaagaaga tgaagaaatt gaagaagatt tagatgaatc
     3661 aattgaatta atatttgaag aattataaat ataaatggaa ggaatttgta taaaaaagta
     3721 aatataaaac tataaattta aaggagataa gaatatgaaa attgtagcag taacatcttg
     3781 tcctagtgga gtagcacata cttacatgtc ggctgaatca cttgaattat cagccaaaaa
     3841 atttggtata gaaataaagg ttgagactca aggaagctct ggaatagata atgaattgtc
     3901 acttaaagat attgatgaag caacttgtgt aatattgact aatgatgttg agattagaaa
     3961 tatggaacgt tttaaaggaa agaaggtagt aagaatgagt gtatctgaga taattaagaa
     4021 atctgatgca ttaattaaga aaataaaaga tacttttcaa taaaaattga tttattttac
     4081 atataactca aagatgtttt taaagaaatt tttattaaaa gcatctttga aaatatctta
     4141 aaaatagaat aaaaggagat atagtcatga aaaagttacc aataaagact gtcgtgcaaa
     4201 gattactaaa cttacaagaa gaggggaaaa gtgcaactct tttaggaatt ggtcctatgt
     4261 caccaaactt gttacaagca agttttgaac tagcaaaaga tgatgatttt cctttaatgt
     4321 ttatcgcaag tagaaatcaa gtagatgcag atgaactagg tggcggttat gtaaatggtt
     4381 ggaatcagga aacttttaca aaagatatta agaaggttgc cgacaaagtt ggatttgatg
     4441 gtttatatta tttatgtaga gaccatggtg ggccatggca aagagataaa gagagaaatg
     4501 accatttgcc tgttgatgaa gcaatggagt taggaaaaaa atcttattta gcggacattg
     4561 aagcaggttt tgacctattg atgattgacc ctacaaaaga cccatttgaa attggaaaag
     4621 taattccact agatgtagta ctagaaagaa ctgtagaatt aattgagtat tgtgaaaacg
     4681 agagaaaaag acttaatcta ccagacatag gttatgaggt tggtacagag gaaaccaatg
     4741 gaggtctgac ttcaactgag acgtatgaaa catttataac tagactaaag gttgaactcg
     4801 ataatagagg tttgccaatg ccaacattta tagttggtca aactggtact ttaactagaa
     4861 agacagaaca agtaggaact tttaatttta gaaatgctta tgatttagct caaatggcta
     4921 agaaatatgg agttggttta aaagagcata atggcgatta cttagatgat gtaactttat
     4981 tagaacatat accatctcaa attattgcta caaatgtagc tcctcaatat ggtactgaag
     5041 aaactagggc atatttaaaa ttagcagaag ttgagtgtaa attagaaaaa gagggcttag
     5101 ttgagaaaac ttctaatatc agacatgttc ttcttgttaa tgcaattgaa tgtggacgtt
     5161 ggagaaaatg ggtagtagga gaacaaaaaa atctaactac tgaagagata tttaaggatg
     5221 aagttttatc taatgaaata ctagatattg caggacatta tacatttaat aatgatgatg
     5281 ttaaaaaaga aatagaagtg ctttatgata atttaagtaa gaataatatt gatggtcaaa
     5341 gatttgttgt agaccatatt aaacgtcctt tgcgtgatta tgcagaatgc tataacttaa
     5401 aaggtgttac tacgaggatt ttaaataaat aataggactt atagtggtga gtattgttat
     5461 actaggtatt tatagttata attatactta ccactattaa atttaggagg actaatattt
     5521 tgattaattt aatttttgat gtagatgata ctttatataa tcaattaacg cctttttaca
     5581 ctgcatataa taaagtgttt tcttcaataa aggatatttc tattgaagat ttatatatga
     5641 gtagtagaaa gtatagtgat gaggtatttc atatgactga aaatggagaa atgccaatca
     5701 aagaaatgca tatttatagg ataatgaaag cttttgagga gttgggaaat tctattacag
     5761 aaaaagatgc acaaagtttt caagatgagt atatatatca acaatcacaa attacactaa
     5821 taccagaagt tgagaggatt ttaaattttt ctaaagaaag aaatattaat ctaggaatta
     5881 taactaatgg cccatcaaac catcaaagaa tgaagttaaa gcagttaaat attgaaaact
     5941 gggttgataa aagtaatatt tttatttcta gtgaggttgg attttctaag ccagatacta
     6001 atatttttag agtagctgag aatgttatga atttagatag agaaaataca tattatgtgg
     6061 gtgattctta tagaaatgat gtattaggtg ctaaaaaagc tggttggaag agtatatggt
     6121 tgaaccatag gggacatgag ttagaagagt tgttttataa acctgatttt gtaattttgg
     6181 aacataagga tttgatatct ttatttataa aaatatgtag ttataaaaat atgtagtaaa
     6241 aaaatgttaa taatagaatt gtataaaata attaataata actatataaa attatatgtt
     6301 tagattgtga gcaatctatt ttatgttaaa aatagattat ttagaagaag gggaaatctt
     6361 aaaaaaattt gaatatcccc tttttattat ttaattaaaa tgaaatagtt agaaatatat
     6421 tttatttagt ttagtcaata acattttggg attctttata actacatgtt aactgttgaa
     6481 taggtttatc atatggttta acccatcctt tattaatact taattctgta agtgctgtgt
     6541 gaccttctac catttgagtt aatgaagctg aatatatagc tcttagttca ggtgtactac
     6601 ttgtcaatgc agaattaaga tacatattgg cagatgcttt agcagatgaa agcatactta
     6661 acattattac tttatcgtct atataagtat tgttcttaat tagattttct atcatatttc
     6721 tcatgttatc tatacctcct cagaaattag tatttcattt tcaactataa attgttgtat
     6781 agctttaatt cttccttctg ttgctaatat actagattct gattgtcttt ttagatcttc
     6841 atctgatatt aacatattta ttgctctttg cataagtaat ccgtcagact ccatttttat
     6901 aattgctgct aaagatattt tttcagtttc tgaaagtatt ctcatttaat catctcctat
     6961 attgtaattt atgctttaat agtatgtctt tcttttcaag gaaataacca ttacactttt
     7021 ttacagtatt ttatattctc gttatatcaa aatttagtcg acttaatata ttaatataat
     7081 tttgtaggat atttatcatt aaatatctat aaaataaatt gtttgaatat tcataaagca
     7141 aaatttattt gtatgtactt cttcaagagt aaatatgagt tttttcaaaa aattaatttt
     7201 tatgataatt attaagggct atatttttct taaagttgtg attatcatta ttaatttaca
     7261 ataaaattgc cgaaaagccc tctcctttag gtgtagggat gaaaggtaag gtcttaatat
     7321 aaaaaagtta gaataatgct tgtaatctaa taccgcta
//
LOCUS       DAYASV010000167         7288 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYASV010000167 DAYASV010000000
VERSION     DAYASV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.172"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            622..1113
                     /gene="infC"
                     /locus_tag="ACGI68_003403"
     CDS             622..1113
                     /gene="infC"
                     /locus_tag="ACGI68_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFH0139806.1"
                     /translation="MNEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLDLVQISP
                     NANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTKANNAIK
                     FLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNNMVVIID
                     PKK"
     gene            1141..1335
                     /gene="rpmI"
                     /locus_tag="ACGI68_003404"
     CDS             1141..1335
                     /gene="rpmI"
                     /locus_tag="ACGI68_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFH0139807.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            1391..1747
                     /gene="rplT"
                     /locus_tag="ACGI68_003405"
     CDS             1391..1747
                     /gene="rplT"
                     /locus_tag="ACGI68_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFH0139808.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            2057..2602
                     /locus_tag="ACGI68_003406"
     CDS             2057..2602
                     /locus_tag="ACGI68_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139809.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKKDEGK"
     gene            2589..2762
                     /locus_tag="ACGI68_003407"
     CDS             2589..2762
                     /locus_tag="ACGI68_003407"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139810.1"
                     /translation="MKGNKSDLHGVEPIYTGNRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            3037..4962
                     /locus_tag="ACGI68_003408"
     CDS             3037..4962
                     /locus_tag="ACGI68_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0139811.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            5363..7267
                     /locus_tag="ACGI68_003409"
     CDS             5363..7267
                     /locus_tag="ACGI68_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0139812.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         3063 a          718 c         1381 g         2126 t
ORIGIN      
        1 atgtagtata gtaacatatt agaatataat gtaggatttg ttatgtagta tagtagcata
       61 ttagaatata atgtaggact tgttatgtag tatagtaaca tattagaata taatgtagga
      121 tttgttatgt agtataatag tatattagag tacaatatag aatttgttat gtagtatagt
      181 agtatattag aatacaatat agaatttgtt gggtagtata gtgttatatt agaatacaat
      241 ataggatttg ttatgtagaa tatatagagt catgtagaat atacatatat aaaaggattt
      301 tgggtataaa tttaaaaatt aatagcaaaa tgagtagtat agacatataa ttagtagtag
      361 gaagtaatta aatttttcct aaacaaaaaa aatagttgca aataatttga ttaagtgata
      421 tagtaaataa ggtaagaata aaacaaagaa gaagtccgct tctcacctta cgccggaggt
      481 tagtaaggta aatataagtt aaagcttaat tttaatttaa gtttgtttaa atttggtaac
      541 ttatattgcg gatgattacg tcatccgctt tttattttat aaaaattttg gaggtgtcct
      601 acaattagca aagaattagc aatcaatgaa caaataaaag ataaggaaat aagagtcctt
      661 tcaccaacag gtgaacaact tggagtaatg cctactaaag aagctcaagc aatggctaat
      721 agcaaaaact tagatttggt tcaaatctcg ccgaatgcaa atccaccagt atgtaagata
      781 atggattatg gaaagtttag atatgagcaa gcgagaaaag agaaagaagc taagaaaaaa
      841 caaaaaacta tagttgtaaa agaagtaaga cttagaccag gtattgaaca aaatgattta
      901 aatacgaaag ctaataatgc tataaagttc cttaaaaaag gggataaagt caaagttgaa
      961 cttagattta gaggaagaga attaggacat aaagacatag gtaaagaagt tatgcttaag
     1021 tttttagata taataaaaga gtttggagaa ccaacaaaag cccctgcatt tgagggtaat
     1081 aatatggttg taattataga tccaaaaaaa tagatgatta actgagagga ggaatttgtc
     1141 atgcctaaaa tgaaaactca tagaggtgca gcaaagagat taaagaaaac aggaactgga
     1201 aagttaaaga gagctaaagc ttttaaaaaa catatattaa ctaaaaaatc tgcaaaaact
     1261 aaaatgaact taagaaaatc aactttagta agtgatggag atgctaagag aatagcacaa
     1321 ttattaccat actaagatac agattgtaaa taataactat agaagaggga tataaaagga
     1381 ggtcataacg atggcaagag ttaaaaaagc tatgaacgct cgtaaaaagc ataagaaaat
     1441 attaaaactt gcaaaaggat tcagaggatc tagaagcaaa ttatatagac cagcaaatac
     1501 attcgtaatg aaagcattaa aaaatgctta tataggaaga aagttaaaga agagagactt
     1561 cagaaaactt tggatacaaa gaataaatgc agctgctaga atgaatggaa tatcttattc
     1621 aagattaatg aatggattaa aattatctgg tgttgaagtt aacagaaaaa tgttatctga
     1681 aatggctata caagacccag aaggatttgc aaaattagca gaagttgcta aagcaaaatt
     1741 agcttagtat tgaatcatga gatgtaaagt ctttgagtta tataaataat tcaaggactt
     1801 ttttattttt tgaaaaatac ttattagtgt catgtacaac caattttata atgataaata
     1861 aaataagcaa tacaatagaa ttttatgaga tttattataa gaattgttac aattatttat
     1921 gatagctata aaaagtcata gtaaaaatga tattgatgta tgttgatata tgtataaagt
     1981 ttccaatcaa tcttgaatta atattatcaa catgataata ttatcttatt aataatataa
     2041 ataaggggga cataatatga atgaaaaact aatggattgt tttgtaaacc caattaaatg
     2101 caagttattg attgaaattt tttcaaataa tcgaactaca gctaaacaat taggagaaaa
     2161 atttcaagat atatcaaagg caacacttta tcgttacctt aataaaatgt tgaaagatga
     2221 tattataaaa gtggttgaag aaaataaaat aaggggaact gttgaaaaag tatatgctat
     2281 gaagttagat atagctgatg atgtgaacaa aattatagat gagaactctg gagaagaata
     2341 tgctcaactg tttactcaat atatgatggg atttttaaga gaattttatg aatacgctga
     2401 aagaaaggaa aaggatatcc taaaagatgg ttcaggattt tctgtctgtc cagtatatgc
     2461 tagtattgat gagatagttg aagtgagtaa aaaaattgct agtatactag aacctctaag
     2521 aaataactca catgaagaag gtagaagaat gcattctata ggagtgataa ttactccacc
     2581 taaaaaggat gaagggaaat aaatctgatt tacatggggt agagccaatt tatacaggga
     2641 atcgttttat gatttacata tgtatccaga gcaaagtatc tctgcttgga ttgtacttca
     2701 ttgtaccatt tcatccaatg acttggagtg gtacaatgaa gttttttaat ttttcatatt
     2761 aatgctaaag aaatgaactt tagaccacat atgcaattag gttgcattat acaattagac
     2821 cacatataca aaataaaaat cagtattttt atagcagata aattgactgt cattcttctt
     2881 aaaaatcatc tattagttat tgttagcata ttgaaagaaa ttatagtaat ctattgaata
     2941 aatgaatatt gcctataatc atataaactg ctatagttta aaataaagca ggacgttaaa
     3001 ttttatagta atctaaaaat ttaagagggg gattttatga aaataaaatt tgttattatg
     3061 ccattggtga tatttttaat ggtattattt gtggggtgtc aaagtacaaa agatacagaa
     3121 gttaaagcaa aagatacaga aattaaaata ttagggacag gtgatttaca ttcaattttg
     3181 acagactcta tggtttcgta tgtaaatgag gaaagggaaa agaataaaaa tctgcttatg
     3241 gtagatgcgg gagatttcta tggtacacaa agtagagaaa tgtgggaatg gtcttctgga
     3301 aaaaagttaa ttaatattaa aagaaatggt agagcagagt atgatgacat aattaagagt
     3361 agtaaagatg aagtacctat agttaaagat atggcaaagt tgaaatatga tgctgtagtt
     3421 ttgggagata atgaatttgt ttcaaatgat aagcaaagtc ttgataaact ggtttctgat
     3481 tttaaaaata ataatatgcc attggtatca gcaaatatat atgaacaatc tggagaaaat
     3541 tatgtacaac catatgtaat gaaaaatatt aagacagatg agggcaatgt aaaagttgga
     3601 atactaggtc ttactataaa agaagtaggg gaaacccttg gtcttgagga ttttgaaaag
     3661 gataaaaaag ctagagagct tggagaacaa gctgattaca aaggaaaatt atatgcaaat
     3721 gatttagtag aagatgcaaa aaaatggata aaagttatgg aaaaagaaag tccagacata
     3781 atagttgcag ttgtacactc aggggaagaa cctaaaacag atagaaatcc aggaaataga
     3841 ataaaagagt tagcaactac agtggatgga atagatgcaa ttgtagctgg tcatactcat
     3901 gaaaaaatag atgaacacaa atataaaaat aaatctggag atgaagtaat tgttacacaa
     3961 ccaggttggc atggagatag agtatcagag attaatttca aactaaataa agagaatgga
     4021 aagtgggaca taaaggaaaa gtctagtaaa ttaaaaataa ttgataaaga agtaagtata
     4081 gttgctactg cagatttaca ttcacatttc tctgataaat taacagttga ccttgttaat
     4141 gaacgaagta acccaattga acctgttgta gtagatgcag gcgacttctt agaccctcaa
     4201 acagatgaaa tgactcaatg gtataaagag tggaaaagta taagagataa taatagtgat
     4261 aaaacgatta gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta
     4321 gtacttggaa atcatgaatt tgtatctgaa aaagatgaat tttggtcaga tgaacgcctt
     4381 ttagatgctg ttatagagga ttttgaacag gtggcaattt ctgtgttatc ggcaaatata
     4441 tataggcaat ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt ggagacgagt
     4501 gaaggtaatg taaaagtagg aatattggga cttactataa aagaagtagg taaaggtctt
     4561 aaaaatgtaa acttacaaga gcaatttcaa tataaggata aattgtatgc aaatgattta
     4621 gtggaagatg caaagaaatg ggtaaaagtt atgaaaaaga aaaaaccaga tataatagtt
     4681 gcagttgtac attcaggaga agaaccaaaa aatccaaaac atccaggaaa tagaataaaa
     4741 gaactagcaa ctacagtaga tggtattgat gcaattgttg ctagtcatac tcatgaaaaa
     4801 atagatgagc atgaatataa aaataagtct ggagaaaaag ttatagtgac acaaccaggt
     4861 gagcatggta aatattattc taaaattacc tttaaagtaa ataaagaaaa gggttcgtgg
     4921 actattaatg ataaattcag taaaacaatt gaggttgaat aactttataa aatttataga
     4981 taaagtaaat tatctgatat tggcatgata tataagataa agtttctaat attagtataa
     5041 tgcataatgt gaaatatttt aataataaga gaataaacaa gacaaataaa aatattttaa
     5101 catgtaagat atagtatgaa ctattatact aatgcttact ttataatttg aacatgttag
     5161 aataagtctt atttgaaaat tttagtgtac tagagtaagt attattttac tagtaaaaaa
     5221 tattgagaac tatatgtaga tttgcttaaa aaggaattta catatagttc ttttcgtata
     5281 ataaatagta caatcttata tggtaaagat aaagtgtgat attaaatttt attatcatcc
     5341 aaaatctaag gaggagtctc ttatgaaaat aaaatttatt atagtagcat caataatatt
     5401 tttaatagta ttacttgtgg ggtgtcaaag tactaaagac acagaaatca atgtactcgc
     5461 cacaagtgat ttacattcaa ttattccaga taatttaatt tcctatgtag aagaggagag
     5521 aaaatatgat gagaatctac ttatggtaga tgcaggagat ttttttgaca tgcaaagtgg
     5581 tgagatgaat aagtggttta ctggtcaaaa attagtaaga tttaaagatg ggataccaga
     5641 gtacaaaaaa atagctgaac cacgtgaagg agaagttcca ttggctaaaa aaatggcaaa
     5701 gctgaagtat gatgcagtta catttgggaa tcatgaattt gtatctaatg acaagcagag
     5761 ccttgataaa ttggtttctg attttaaaaa taacaatata ccattagtat cagcaaacgt
     5821 atatgaacaa tctggagaaa actacgtaaa gccatatgta atgaaaaata taaatactga
     5881 caagggaact gtaaaggttg gtatacttgg tctgactata aaagaagtag gggaacgttc
     5941 aaggtttaaa agttttgaac aagataaaaa ggctagagaa cttggggaac aacctcaata
     6001 caaagggaaa ttatatgcaa atgatttagt agaagatgca aataagtggg taaatgttat
     6061 ggaaaaaaaa gaaaagccag atataatagt tgccattgta cattcaggtg aagagccaaa
     6121 aaatcctaaa aatccaggaa atagaataaa agaactagca acaactgtag agggaataga
     6181 tgctattgta gcaggtcata cacatgaaaa aatagaacag catacatata aaaataattc
     6241 tggtgaagaa gtaatagtaa cacagcctgg agctcatggt aatagtatat caaagattaa
     6301 ttttaagttg gataagaaga atgataagtg gtttataaaa gacaaatata gtgaattaaa
     6361 ggtttttgga aaggaagtaa atataattac aacttctggt ctgaatccaa aattttcaga
     6421 tgaaatggca gttaatttgt ttaatgaaag agatagagat gttcaaccta tctttataga
     6481 tactggagat tttttaaata ctaatacaaa agaaatgact aagtgggcta aagagtggca
     6541 atacaatgaa aataaaggca tctataaaaa aataaatgaa tggccaattg tatatcttgg
     6601 ttatgatgct gtagtacttg gtagccatga atttacagag gataaagaga cttttgagga
     6661 aaaaaaatct acattggatt atattataga aaactttgaa gaaatgaaga taccagtatt
     6721 atcagctaat atatatgatg aatctggaga aaattatgta aaaccttata taattcataa
     6781 tgtagaaact agggaaggaa atataaaggt tggagtatta ggtcttacta ctactgataa
     6841 ggatgaagaa gacttaagta actcaagctt aagcaaagac tcaaagactt cgaaaattcc
     6901 tgaagaaaca ggaaagttat atactaataa tctagtaaaa gatgcaagcg aatgggtaaa
     6961 agttatgcaa aaggaaagtc ctgatgtgat tgttgcagtt gtacattcag ataataatag
     7021 tactctaaaa aatccaagta gtgaagtcaa aaaacttgca atgtcagtag atggaataga
     7081 tgctattgtt gtaggtcata cgaaaaataa ggtagaagaa catatttaca aaaataagtc
     7141 tggggatgag gttatagtaa cacaatctgg agaagattgt tattcaaaaa taagtcttga
     7201 actaagaaaa gaaaatggca agtggaatat cataaacaaa tatagtaaag caataaaaat
     7261 gaagtgaatt tattgatata taaaccat
//
LOCUS       DAYASV010000168         7278 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYASV010000168 DAYASV010000000
VERSION     DAYASV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.173"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..520)
                     /locus_tag="ACGI68_003410"
     CDS             complement(<1..520)
                     /locus_tag="ACGI68_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0139813.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPKGRILNATISKEPSGR
                     YYVSLCCIDVDIE"
     gene            complement(1169..1576)
                     /locus_tag="ACGI68_003411"
     CDS             complement(1169..1576)
                     /locus_tag="ACGI68_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HFH0139814.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(2050..2934)
                     /locus_tag="ACGI68_003412"
     CDS             complement(2050..2934)
                     /locus_tag="ACGI68_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0139815.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(3225..3701)
                     /locus_tag="ACGI68_003413"
     CDS             complement(3225..3701)
                     /locus_tag="ACGI68_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HFH0139816.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3967..5142
                     /locus_tag="ACGI68_003414"
     CDS             3967..5142
                     /locus_tag="ACGI68_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HFH0139817.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(5223..6026)
                     /gene="proC"
                     /locus_tag="ACGI68_003415"
     CDS             complement(5223..6026)
                     /gene="proC"
                     /locus_tag="ACGI68_003415"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFH0139818.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(6066..6656)
                     /locus_tag="ACGI68_003416"
     CDS             complement(6066..6656)
                     /locus_tag="ACGI68_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0139819.1"
                     /translation="MVLNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLT
                     SVATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLK
                     EKTMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYN
                     SEKNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2386 a         1216 c          806 g         2870 t
ORIGIN      
        1 cttcaatatc tacatcaata cagcataatg atacataata tctaccactt ggttctttag
       61 atatagtagc attaagtatt ctccctttag gtacttgttt atcccttatc ttaaccatac
      121 caagtttagg tagttttata tgttgaccac aatacataat gtttccattt gtaaagttag
      181 ttttatatga aaatctatta attttctttg atttaaattt aggaaatcct gctttttctt
      241 taaagaattt tttatatgca ttatctaaat cttttaaggc gttttgtagt gaaaatttgt
      301 caggttcttt aagccatttt aattcttttt ttaaattggt caaatcagaa ctacactgtt
      361 tataagtaaa agtttcttta tcgtttttat aaacatctat tcttttagca agatacttat
      421 tatatacaaa tctacaacat ccaaaagttt tgttaattaa ctcttgttgt tttttatttg
      481 gatacattct aaacttataa gcttttttca ctgctatcac ctcactttaa ttgttttttg
      541 atacactttt taatgatttt atcacatttt atcacattta atatattata taatgctgtg
      601 gtgaacaatt tattaatcac ctataataat gttaacaaat gtaattattt aaaacaatta
      661 tttagaaggt tttatattta attttaaata acatatttaa gaaccgtaag tcttaacttt
      721 tatcattttt gaggttgtcg ttcacataag ttcgctacta cctatgcagt tctcttatga
      781 acttcttaca ctttcatgta agcacagact atatcttatc cttcagcatt atccgttaag
      841 gtctacccac ttccactacc aatagcttgt agtgtacttc cctcaagagg aatagtcgtt
      901 gaagtttctc ctattcggag tttacctgct gattgcccat tttaaagtac ttaggattta
      961 accttatacc atctaaccaa ttttttctac tttcgtaact ttcacactta ggtttatttc
     1021 atccttatgt tttagtttga ttagctttag gggtttccag caattcaagt agtattggat
     1081 aggttctatc ctatctctac atataggttt ccctatatgc tgactatttt agctactaac
     1141 tcatgactaa agtcacgagt gtgcgtagct attttttaat caatatttta gtaaatttta
     1201 cggtaagtaa aaacctctct ccttcaaatc ctatagcaaa tatacttaca agttgctttg
     1261 agaaaataac tcctaatata gctacaacta tgcatatacc aactattata tttaaaacat
     1321 tgtttaaaaa ctttagtgct tgcttttcac cttgtttact acttatatct tgatacattg
     1381 gaataaaagt agtaactata gcagtaccta ttgctgcaaa tataatattt ggtatattca
     1441 tagctgttaa ataactctct gtatataagc ctgtaccata aattgatgat aatactaatt
     1501 ctctacccat tccaagaatc ttagagatta tagtaactat catcaaataa aaagttgctt
     1561 tagctacctt gctcatttta tcactccact tctaacatac ctctttttaa tcaagtaaaa
     1621 tttagctcat tagatataat tctatcaact aatttgcctt gcttagccct atcaaaatat
     1681 aattaacacc tatttcttaa tatatcacaa actacattat tcttcatatt tttttatttc
     1741 tccaataagt tttacatttt tcctatataa attgtatagc ttcaattata ttcaaagaaa
     1801 tggagttgct acccgataac tttaaaagta aaaaacagat tttattatct ttttaaacta
     1861 tattctaaca tattttgtcc atattgagaa attttactaa actattatgt actttattaa
     1921 ataaataaaa taacagcaca cctataaaat acaagtgtgc tgttcataaa cagattttat
     1981 aaaaaaataa ttcctaagta gagattaaca tgctttctca tactctcata tataatgtgt
     2041 ctaaaaatat taataaaaat gctttaattg ttttaactta cttggatgtc ttaattttct
     2101 tattgcttta gcttctatct gtcttattcg ttctctggta actccaaata cctttccaat
     2161 ctgttctagt gttttagggt catcatcatc aattccaaat ctcattctta aaacttgctg
     2221 ttcttgctct ccaagacctg taagtagttc ttctatcttt tcttttaaat tatgctgttc
     2281 aacttcgtgt attacaacat ctttaaaatc agcatctgat ggtataaatt caactaaact
     2341 actatcctca tcttctttta atggagtatc caaagataca acatttttat ctcttcttaa
     2401 taattccaac actttgtcaa cttctaagcc acatgcatct gcaatctcat ctatagttgg
     2461 ctctcttaat aatacattta aaagttcttg tttctttttc tttacaaaat ttattctctg
     2521 atgaagatgt ataggtatcc taatagtatt ttcgcaatcg tcgatatatc ttgttatact
     2581 ttgttttatc caccaagttg cataagtgct aaatttgtat ccttttttat aatcgtactt
     2641 ttctacagct tttataagtc ctatatttcc tgcttgaatt aaatccaaca tatcaatact
     2701 atctctttta tatctctttg ctatgctcac taccaaccta taatttgaat ttataaattt
     2761 ttcttttgct actgaagatg agttgattat ttccttagcc agctctacct cttctcctgc
     2821 tgacaacatt ttgtattcct ctatttcctt taaatacatt ttcatcgcat tcgatacatt
     2881 tgatgaaccc acacaaatat aattttcata attattttct tttttagtat ccataatcaa
     2941 accccttcta ttcatagaat ttgctaaaac aaaaataaat aatttaaacc tacataatct
     3001 acaattacat aatatcccat tctttaaatg aattgttttg tatattatgt atacaaattt
     3061 ataaaaacta tacctataat attattctac ttctaacagc aatatcctcc ttatatatat
     3121 aatttatttt attatatgtt gttatattct tagttttttg aatgatatat gtctaaaaaa
     3181 agactgttta ctaaacagtc tttttttata tatatgctca atatttaatt ttcatgatga
     3241 tgattacatc cacatccaca attttcatca tggtgatgat gttcttctaa atcttttacc
     3301 tgttgttcca tttcatgaaa ctcttttacc attatatcat aagttggaag agctgcctca
     3361 gtatcgtatt cttcccctct ccaatcagcg tacatcatag catctccatt agttaaaacc
     3421 aatctacctg acatagttat attatccact tcttctaaaa attctatttg tttttgtaaa
     3481 acttctcctt ctaactccac atcttcatct aaacattgca ctatcattac aggtgcataa
     3541 taatcttctt catctttaac atttacaaca atatctaaaa ttccttctgc accttcttca
     3601 aattctgcca actcaacatt tgtatcaatc atatcttgag gtactaataa atcttctatt
     3661 aaatatttta ttactctatc ttttactaat tcttttgaca tttatttttc tccttccgat
     3721 tctataacat tatcataatc atttctatat tatagtatac tacattaatt gttttttcaa
     3781 tctcttacaa aaattatttt ttattatctt ccttatattc ccttttttat tacaaagttg
     3841 taatcgcatt gatttaaaaa atagtttata tttagtataa tagaaatgta gcacatattt
     3901 ttattcaata tattaataaa aaaatatatt gaaaatgtaa tgtatgttaa atctaggagg
     3961 aatattatga agtttaaaaa acaaattagt tctattgttg tgtctactat gcttgtactt
     4021 gggagtatga acctttctta tgcagacggt acaaatgtag ttacacttgg agctaactta
     4081 tcagagtctc aaaagcagca aatgcttaat tactttggag taaaaaaaga ccaagtctta
     4141 gttttagatg taacaaatgc agaagaaaga cagtatttac aaggtgttgc aacagagggt
     4201 caacttggaa aagttactat ctcttgctca tacgttgaac ctactaaaaa aggaaatgga
     4261 attaatgtta aaacagcaaa cttaacttgg gtaactagtt caatgattgc ttcaaccctt
     4321 acaacagctg gtcttgaaga tgcaaatgtt attgctgctg ctccatatcc tgtaagtggt
     4381 actggagcat tgacaggaat catgaaagca tttgaggatg cttcaggaaa gaaacttgat
     4441 gaaactaaga aagaaattgc ttctgaagaa ttggtagtta caggtgactt aggagatgat
     4501 atagggcaag aaaaagcaac aggtgtaatg aatgatataa aaacagaaat tgtaaagaat
     4561 ggtacaaaag ataccaatca aatagcaaat acaataaata atgttgtaaa taattacaat
     4621 attactatta caccagaaca aaaagaacaa attaaaggtg taatgaaaaa aatagctgac
     4681 caaaattacg attacaatag catgaaaaat acactagata atgtatcaga taatgtaaat
     4741 gaacaattaa agaaattagg tgaaagtgtt aagggttctg gtatactaga tactattggt
     4801 ggatggttta gtggaatagg agactggttc tctggtttat tctcaggcgg agataaaaaa
     4861 gatttgggaa tcttaaataa tactaatgat gagtctcttg gctctgatgc tgtaataaat
     4921 tctacagaag gtgttcaaat aaatccacaa aataatacta attctaacaa tgatagtgat
     4981 acaaatagct caaaaaatga tacttcaagt aataatgaaa ataacgatag ttctcaaaca
     5041 aatgaaaaca acacaacaaa taattcagaa aatcaatcaa ataataatga ctcagttggt
     5101 ttctttgaaa aaataaaaaa ttggctttca ggcttatttt agaaattaaa caaataaaaa
     5161 gaattgccta aaatggaaat tttctataaa aggcaattct ctttatttac aataaatttt
     5221 tattactcac tcattttttt agatttttct atacatgcac atattgcttt tatcacagaa
     5281 gctctaaaac cttcttcttc aagtacttta actgcttcta tagtagttcc accaggtgaa
     5341 cataccatat cttttaattc accaggatgt tttcctgtct caagtaccat ttttgcagaa
     5401 cccataacag cctgtgatgc aaacttatat gcttgtggtc ttggcattcc tgcaattact
     5461 gctgcatctg ctatagcttc tataaataaa aatacatatg ctggtgcaga accacttgca
     5521 ccaattacag cttctataag atactctggt actacctcag tatttccaaa agaattaaat
     5581 acttctgtaa ctgcttctaa gtcttctttg tttatatttt tatttatcga aagagaagac
     5641 atggcttcat ttacaagagc tggagtattt ggcatagttc taactatttt tttatcttct
     5701 cctataatag actctatatc ttttatagat tttcctgctg ctattgtaac tataatttta
     5761 ttgtcatcta taaagtcttt tatctcttcc aaaatatcat catatatatc tggtttaaca
     5821 gctacaatta ctatatcaga ggttctagtt acttccttcg agtcttttgt agtatttatt
     5881 ccaaaatttg catctattct atcgagagtt ccttgactat atgctgatgc tataaccata
     5941 ttaggttcaa ctaattttga atttactatt ccccctatca tagcgcttgc catatttcct
     6001 gcacctatga atccaatttt tttcacttta gtacctcctt attgactaat agtaatcgtt
     6061 aaaatttaag cttctttaac tgcatcagat ttttcaaatg gatttggtat acttactacc
     6121 aaaattttca ctaccttatt agtatagttg acccaattat gattgttctt ttcactatta
     6181 tagtgtattg tgtctcctgg atacatttct atttgttcat ctcccaaaaa cactgtaaga
     6241 gtaccctcta atacatacac aaattcctca ccttcatgta cgtaacaaca aatttcctca
     6301 ctactcttac ttggaagaat ttcaattaat cttggtagca ttgttttttc ctttaaacta
     6361 gatgataaat gataatgtat aaatgttgag ttttctacat caaatacttc tttttcataa
     6421 cttcttaata cagctctttt atgctctttt ggcttcataa aaaagtaagt taattctaca
     6481 tctagtacac ttgctatttt tcctaaggaa tctgttgcca cacttgttaa tcctctttca
     6541 agttgtgata aaaaaccaat tgacaagttt gttttttcac tcatatcctt cagcgtcatt
     6601 tgcttttgag ttctgagttg ctttattttt gctcctatgt ccttattcaa aaccatcacc
     6661 cctaaataat tttaattttt tcataatata aatacaaaaa tataaatata ggtactgaac
     6721 ccatctttat ttcatttaaa aaaattttta taagtattac tataatttat caatcttttt
     6781 ttgtctacat ttttacgatt atttacgatt tatttttcac atatgtatat ttaatcttgc
     6841 caactaaaga tacatttttt ataagcattt actctttata taataaaact taagttgttc
     6901 atgtagtctt cttctggttt aattattaaa atttcaaatt atattattat ttctacacat
     6961 tattacatcc tttagtacaa ttataacata attttacaat ttaatgatat ctattcaatg
     7021 tcttttttta ctctacaaca tattttattt tagagtttaa tactatatat attttttata
     7081 tacagccttg tcatttttta gattgtcaag agttattgaa gtattttatg tagatttaga
     7141 ttttatttcc taatttctca tttaacaaga attgcttatg ataaaaaata atatcatctc
     7201 aataagattc ttgtaaatat atctatattc taaatataat ttcttttttt gtcaaaattt
     7261 cttcattctt taataatt
//
LOCUS       DAYASV010000169         7269 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.174, whole genome shotgun sequence.
ACCESSION   DAYASV010000169 DAYASV010000000
VERSION     DAYASV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.174"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(90..641)
                     /locus_tag="ACGI68_003417"
     CDS             complement(90..641)
                     /locus_tag="ACGI68_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HFH0139820.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(792..1055)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1491..1889)
                     /locus_tag="ACGI68_003418"
     CDS             complement(1491..1889)
                     /locus_tag="ACGI68_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139821.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILSIIVIFLVIVLVVLIFIKRKKKKFY"
     gene            complement(2245..2730)
                     /locus_tag="ACGI68_003419"
     CDS             complement(2245..2730)
                     /locus_tag="ACGI68_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFH0139822.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
     regulatory      complement(2813..2906)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3461..3643)
                     /locus_tag="ACGI68_003420"
     CDS             complement(3461..3643)
                     /locus_tag="ACGI68_003420"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139823.1"
                     /translation="MLNKKLPDTELKIMKCIWHTGYKTVISKDVADEIEKTYGWKHTT
                     TIILLARLTKKGFLIS"
     regulatory      3869..3966
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4021..4197
                     /locus_tag="ACGI68_003421"
     CDS             4021..4197
                     /locus_tag="ACGI68_003421"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139824.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            4423..4623
                     /locus_tag="ACGI68_003422"
     CDS             4423..4623
                     /locus_tag="ACGI68_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFH0139825.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            5375..5557
                     /locus_tag="ACGI68_003423"
     CDS             5375..5557
                     /locus_tag="ACGI68_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFH0139826.1"
                     /translation="MFKFYNNATLMFLSGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIIF"
     gene            5628..6473
                     /locus_tag="ACGI68_003424"
     CDS             5628..6473
                     /locus_tag="ACGI68_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0139827.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNEDPKYNLNTI
                     KKHLN"
     gene            complement(6697..6861)
                     /locus_tag="ACGI68_003425"
     CDS             complement(6697..6861)
                     /locus_tag="ACGI68_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139828.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         2646 a          964 c          740 g         2919 t
ORIGIN      
        1 caacataaga ataaaatagt tataatctac tttattctta tgttatttag ttattataat
       61 gctttataaa gtaaaatatt atttcgtgtt taagcttgat aactaactct tcttattgat
      121 agactaacaa tcttgaataa aaatattcct gcaactagat ttattaaaac agctggtaat
      181 actactgcta agaataaagc tgtaaatgaa gcccctggaa gaccaactat agcttgagct
      241 gctaataaaa agatagttcc acttacaaga gttcctattg gcattattat tgcaactact
      301 acagtatctt gtgttttctt agctaatttt tttataaaag gcatattata actaatattc
      361 attaataaaa atactatatt tgctgtaaga gttttatcta ttacattagg aatttgacct
      421 cccggaaaac ttgatgtaag tgctgtaaat attcctgtta ctatacccgc tatcaaacaa
      481 gttttataat catctctatt taaaaccaat attacaaata acattgctag tggtacatct
      541 ggcttcattg gtaatcctat agctggtgtg atttgatgta ttaataatcc catcgctaaa
      601 agtattgcat ttaaagttaa tttttttgta tttgttttca ttttttgtac ttcctcctat
      661 ttttttaatt ttcaatttat ttttttgcca acctactata tcatcatctt tagcttttga
      721 aattcataac atctttctca ccaccttcta tcttttaaat tttcagtaaa ataaaaaact
      781 ccgtcctaaa ctaataggac ggagattatc cgcgttgcca cctaaattat ataaaaatta
      841 atataaaatt caattttcat acatctcatt atcaggtact tacatacctt attcgctata
      901 acgtgcgact gacgataaag tctactctca aaactatgat ttcaatttat tcctctaagg
      961 gccattcatc aaattttttc ttgctaagat tccaccatcc tcagctctct atactgaacc
     1021 taaatttaac tactcttcct tgtcatagga tttaatttat tgtattgaat tttgttgtta
     1081 aaataatgat attctttttt ctaaaaaatg tcaatacctt tttataaaaa atatattttg
     1141 taatctattt tttataaaaa aagtaaatta tactatacat ttcttttcat atacaaaatt
     1201 ctatgaataa ttactttaat ggtctaatag tctgctcttt ggctctaatt cttcttatat
     1261 taattttgtg aataactgtt gatgtaattt tatattttga ttcatgagga attaaattct
     1321 tcaaagttaa ttaagtacat ttatcaataa atttacttaa ttaaccatca tttaatactc
     1381 attcctgtat ttatatgcct cctttgaaac agaatattac tccattttaa ttacatcata
     1441 ttattaaata gataccaatg attaaaaaaa tgatattctt ttaattattt ttagtaaaat
     1501 tttttcttct ttcttttaat aaatattagc actactaaaa ctataaccaa aaatatgact
     1561 attatggaca atattaattt tgttaggata ttatgtcttc ttgcgttttg tcttttatat
     1621 ttatttatag tttgatatcc atacttttta gttcttatac ttcgtttttt caactttact
     1681 ttacttttat tatatttcct acttttatgt ctacttttag atttaatagt tttaaattta
     1741 gtattatcat atgtataatt ctcttttatt gaagaaatgt atacactaga ctcaataata
     1801 gcttctgctt ctacttgtaa tggatttata atgctgatta taagtagaaa agatatagct
     1861 atcccaatca attttttctt cttattcata ttttccccct cataaaaatt tttaactagt
     1921 tcattataat atatatataa ctaaaaatta caaatttttt caagtttctt atatatctta
     1981 ttatctttaa tattattttt attatcataa cttttacttt ttttaagatt tttcaaagta
     2041 gtattaattt atgatacttg gtgtataaag tataagttaa atctactttc aattaaattt
     2101 accatttata attactttat aataaaaata aattagttaa tttattctct ttagttctgt
     2161 tataatgttt ctacaaaaaa gaatcccatt aatgggattc ttttttgcga tttattaata
     2221 tatttaaagc ttgctagtat cgtattataa acttaaatgc tcttctatgt attgattata
     2281 tacatatcca gaatatcgtt tttcattttt attgatatta actcttgccc attttccaac
     2341 gctataacat acttcaactt taaaaccatc agggattgta gttataactt tagattctaa
     2401 tgttctatca tttctaagtt ctaaacttaa tccatttgtt cttatatttc ctatgtaatt
     2461 cttttttcta ttttttattg tatcattact attgttttta gcattactta atctttctaa
     2521 atatttataa agtaaccaac ctttgtcata attactctca acttctgccc atattccatc
     2581 tacacacttt aacttaacaa ttttaccttc tggtatactt tttaatttca aactatttat
     2641 gtctggtttt tctcttaaat tcaatttaat tttagtctta aaattgatgc tagatttgcg
     2701 aaaatttttt aacattaatt cagatatcat cttaagccct cctcttgcca aatattaagt
     2761 tattaaaata tataactcta aaaatacttt taacaaaaag aattattatc tttttggcaa
     2821 ctggctgaca tgacctttat tacataaaga ttttagtccc tatagttttg cgtcattaga
     2881 tttatctaat attgcctata tctgaataat aacataattt gaacaattaa aaaataatat
     2941 tacctcaaat tttatattat ttaaatataa attaaatttt taattaataa taatcaaaaa
     3001 atatctttat atttattcaa ttactgttct ttatagtcaa tataaaagac agtaatttat
     3061 ctatagtgtt acttatattt tatatatatg gctatttttc aacattttac aatatataac
     3121 atttattttt ttaacttttg tcagtaaaat gtcagcaaaa cactttatcc acaattttta
     3181 tataagtttt ccacaactta tcaccaatat tatccacata aaaaaggtag cacttatatt
     3241 attagctacc ttttatttat taatctttat atttccagct ttttatccaa cttcctattt
     3301 ctattatttt ttcttcagtt atttcttctc catcatgtaa ttttgaaatt aattctgata
     3361 aaggattgtt atgtaaacct ctaaaaactc tttttccctc tattttgaga tattctctct
     3421 cttttattaa tactgtatag tgtacatgcc tcccaattct ctatgatatt aaaaaacctt
     3481 ttttggttaa tctagctaaa agtataattg tagttgtatg cttccaacca tatgtttttt
     3541 ctatttcatc tgcaacatct tttgatatta cagttttata accagtatgc catatgcatt
     3601 tcataatttt taattctgta tctggtaatt ttttatttag cataatatat tccttctttc
     3661 aatttatgtc acttcttcaa atttatctag cttaaattat aataggagct ttttagaaca
     3721 aaaactatat ttaattttta ccaggtactc tacgtgttta gtctgttttt aagtgtaaaa
     3781 taatgttcat atctataaat ttatttaact ttcaaacaat cctgttgaat aataactaat
     3841 tctattgtat tattttagtt gtagaataaa actaaatcgg caaaactaga gaaatttagt
     3901 gacgcaaagc tatagggact aagacttata taaatatctt atgagttatg tcagccagtt
     3961 gccaaagaga tagtgttctt tttgttttta tgaaagtttt ttagggggat aataatttat
     4021 atgtttggaa ataaaatgga taaatgtaca catgtattga ctgcttatat tagcagttcg
     4081 tatgattatt gtaatttttt agatacacag ttagatgatt ttatattaga gtacggagaa
     4141 aatgtagtag agtcttgttt acaccaagta atggtattgg taagtaagta taattagata
     4201 gacattgatt tctagaaaac cctttttagt tttatctaca tttatattaa tatatagcaa
     4261 tgtaaaacgt ttaaatatat ttttaatatt aagaataatt aaaatgttaa ctaaaaaata
     4321 taattaagta gacatttcta ttaatctatg atacaatagt atttgtggta atacacaatt
     4381 aaatcaagta tatcttgata taggttaata ggagtatttt taatgaataa tggaatagtg
     4441 aaatggttta ataatgaaaa aggattcggt tttatatcaa tggaaggtag agatgatgtg
     4501 tttgctcatt tctcagctat acaaacttca ggatttaaat cattagaaga aggtcaacaa
     4561 gtaagctttg atatagttaa aggtgctaga ggtcctcaag cagaaaatat aactatatta
     4621 taatatatag atttttcggg aaagactctt tttagagtct ttttttattg ttaatatatg
     4681 taagataatc attggtgaag taaataaggt atattgagta atgttcttga tagtttatat
     4741 gctaatgaat ttcaaaaaaa taagcactcc tataaaaaga gtgctcttaa tgtaaatata
     4801 aaccttcatt ggtccagcct aataccccgt attctccaag ttctaactat tttttatata
     4861 cattatcact ttcatccata tctaacacaa ttatttcttc tttagaatca atttgtaaaa
     4921 tatgaggact gtgtgttgtg acaattattt gtgtttttag gagaattgct tttaatgtaa
     4981 taaaaatttt acataccaca tagttgatat aaaactataa taattacata agattgtaat
     5041 aataagactt atctttacat accacatagt tgatataaaa caatgaaatt aacatacaag
     5101 agactaagac aattaaaatc tttacatacc acatagttga tataaaaccc caaaataaac
     5161 ttagcatctc caatgtttac acatatacaa tgctcttaaa attgcagtga acgcgcaata
     5221 gtgcaattga taatacttat tatatattct caatgcctta tatttcaact gctaaacctc
     5281 aatttgtcac aaatatcgct cactgcaaaa tttctatatt tttattatat cataacatat
     5341 aattaatatt ttcaattaat ttttacattt aattatgttt aaattctata ataatgcaac
     5401 tttaatgttt ctttctggaa ctaatataaa aactatttca gaaagattag gtcatgcaga
     5461 tataaaaata actatgaata gctactctca gtttttagaa gagatggaca gagaagcatt
     5521 tgaaaatttg agcaaaatac tatttattat attttaaaga agatgactta ttcaatattt
     5581 gatgctagaa tataattaat aaaaatttaa ttataggtga ttgtattatg aatattatta
     5641 aaagcataac tttactctta acattattaa ttgtgatatt aatgacaggc tgttccccaa
     5701 gagaaagtgc tagtgaagtt gcaaaaaaat atattgataa gatgaactct ggtgactatg
     5761 aaacagcata tagtttatta agtaaagata gtaaaaaaaa aattagtata gaaaatcttg
     5821 aagaatttca aaatatttta gttagtacaa aaagaataat tagttataaa ataggtaaag
     5881 aaaaaatcat aaaaaaatat aaacatgatg gaaaagaata taaaaatgtt gttaaattag
     5941 aagaaacttt taatataaaa aaccttcttt ataaaaaaga tgattctcta aaagtaaata
     6001 gatattttgt agttgaaaat aacaaataca agctattatt gtatgaaagt ttcaaaaaag
     6061 aatttggtat taatagtatg gaccttgctc aacttaagct taatgatttt ggactaaaat
     6121 actttaaaga agttgacctt atactaaaaa aagctattgc tattaatcct actgattcaa
     6181 agctttatta tgcccaagca tgtaattata tgaattttga tggatttcct ggttattcag
     6241 atacaacata taatgcttta gattcaatta ataaatcttt aaagtattta gataaaaatg
     6301 attctgacta caaaaaaaat gcaagtaata tatataactt aaaaggagtt attcttatgt
     6361 ttcacaaaag atttgatgaa gcacaacaat atttaaataa agccttatac tttaacaaag
     6421 ataatgaaga cccaaaatat aatctaaata caataaaaaa acatctaaac tagatattta
     6481 atttttgaat ttataaaata tttttttatg tataattcag cactgaatca taatttctac
     6541 taaactaatt acaagtttaa attataaaga cttatttcta tataaatatt actctataac
     6601 aataagtttc ttatttaaat tttaaaatat tatctaaaaa tcataaaaaa gaaggtataa
     6661 agagctaccc tcaagctcaa tataccttat aaatatctaa acttcatttt ttactaattt
     6721 ttctatatct ttgataatat gttctttaga tttttttcca gctatggcat caaaaattgc
     6781 aacagccttt ttctctccaa acttttctgc tacatcatta aagtctaaaa atctaacact
     6841 ttcttttggt ttaccaaaca tataacacct ctcatacatt tttatatatt tattataact
     6901 tctatagact tatttttcac caataaacgt tagacaaatt agtcccttta tttttttaaa
     6961 ctagctttac cttcaaaatt cttaacaaaa acagtttatt ctactctata aataaaaata
     7021 gacaatttaa tttgtaggaa actgataata gaatacagta aataaaactt tgtttaatct
     7081 atctgaaaaa gttatctatt aaaactgcta tgaatttata aagaatattc tagttcataa
     7141 aaatacaaat atgtaaatat aatcacctta atattcaatt tatttaaaat aaaaaatgta
     7201 taaaagattt aataatgaca atatactatt ctcaatacaa tctatgatgt aaataattta
     7261 aaactacta
//
LOCUS       DAYASV010000170         7168 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYASV010000170 DAYASV010000000
VERSION     DAYASV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7168
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.175"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            62..913
                     /locus_tag="ACGI68_003426"
     CDS             62..913
                     /locus_tag="ACGI68_003426"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HFH0139829.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1046..2659)
                     /gene="ggt"
                     /locus_tag="ACGI68_003427"
     CDS             complement(1046..2659)
                     /gene="ggt"
                     /locus_tag="ACGI68_003427"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFH0139830.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2884..4821)
                     /locus_tag="ACGI68_003428"
     CDS             complement(2884..4821)
                     /locus_tag="ACGI68_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139831.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKTIELIDKDINIEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEVEKINLHIKDTNEYDINLFKVQKSILEPEIN
                     SQATFDLIVAKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYNNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4808..5575)
                     /locus_tag="ACGI68_003429"
     CDS             complement(4808..5575)
                     /locus_tag="ACGI68_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139832.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5864..6538
                     /locus_tag="ACGI68_003430"
     CDS             5864..6538
                     /locus_tag="ACGI68_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFH0139833.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6562..>7168
                     /locus_tag="ACGI68_003431"
     CDS             6562..>7168
                     /locus_tag="ACGI68_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0139834.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYS"
BASE COUNT         2536 a         1041 c          848 g         2743 t
ORIGIN      
        1 ataaagttaa taattaatat aaattaaaat aaagataata taaacacagg aggtacattc
       61 tttgaaaaac attatttcag caaaagaact tatatcaaaa ctagaaaaaa atgataatct
      121 agttgtaata gattgtagat ttgatcttat caatagaact tatggtattg attcttataa
      181 aaaaggacac atcaaaggca gttttatctt agatatagac aaggatttat catctcctac
      241 aaaagaacat ggtggtaaaa atcctttaca agacccttta atactaaaag aaaaattaga
      301 aaaaatgggt gttgataatg acactacgat tgtaacttat gatgatggag atttaaatgg
      361 agcttgcaga ttatttttcc aattaaaaca tttggggctt aaaaatgtct atgtactaga
      421 tgggggaata acatctttcg taaaggaagg tggagaattg gaagagaaag tgaatatacc
      481 aagttgtaca ggtaaggaaa ttagaccaaa tataaacaat gatttagttg ttccaatgga
      541 atatgttaaa tctaaattat acaaaaaaga tactgtaata atagattgcc gtgctaatga
      601 gagataccag ggtttagttg aacctgctta ctcaaaagct ggtcatatac caagtgcaaa
      661 aaattatttt tgtaaggatt taataaaaag tgactttgaa aatggctcac ttaaagatat
      721 agaattttta aaaaaattct ttaaagattt aaataattat gatgaagtaa ttttatcttg
      781 tggttcaggg atatctgctt gtgttaacag tcttgctctt agagaactgg atatacctca
      841 caagatttat attggtagct ttagtgactg gattagttat gatgacaatg aaatcaaaac
      901 tggtcaagaa taaggctcat aatctatata aaatcagcat atgatagctt aaattttgta
      961 aaggcgtcag tcattgtaga ctttcgcctt tacttattat ttcatatact ctttaaattt
     1021 attatatata ttttagttga taatattaat aaactgcaat atgaccatca catcttgatt
     1081 ccgtcccaca aatataactt tcctgttctg ttttaaatat tatttgacct cgtcccatgt
     1141 cgacagtatc atgaattact tttatttcat gacccttttc aattaatgaa tctattatat
     1201 gctttggcat ctctggttca acttctattt cttttccttt tatccactgc catcttggag
     1261 catctagcgc ctcttgtgga tttaatccaa aatcaatcat atttgttaac acttgaatat
     1321 gtccttgtgg ttgcataaaa gctcccatta caccaaatgg acctattgct ttatcttctt
     1381 tacctaaaaa tccaggtatt atagtatgat aaggtttttt aaatggtttt actacattgt
     1441 ggtgtttagg gtctaaatta aaattatttc ctctactatg taaagcaatc ccagttcttg
     1501 gtatcacaat tcctgaacca aaatttatgt aattgctctg tatatatgaa accatatttc
     1561 catccttatc tgcagtacaa aggtatactg taccaccaca atatggctct cctgcagttg
     1621 gatacaaagc ctctttattg tcaatcaata aacttctttt tttagcatac tcttggctta
     1681 gtaattcctg aatctttacc ttcatatgct ctatatcagt tacatatgtt ttagaatctg
     1741 caaaagctaa ttttaaactc tctataattt tatgtatgtc ttctacattt tctatatttc
     1801 cttctaattc caattctttt aatatgttaa gtgccattaa aactgttatt ccatgaccat
     1861 ttggtgggat ttcaaatatt ttatatccct tatattctgt acttataggt tctacccatg
     1921 ttggataaaa acactctaag tcactctttc ttatagcccc attaaatttt ttagaatatt
     1981 catctatttt atcagcaagt cttcctctat aaaagctttc agcctgagta tttgctatct
     2041 cttctaatgt acttgcctgt tcttcacata taaatatatc tccagcctct ggagctttac
     2101 catctttaga aaatgtatca aaccatggtt tgaactcttc tccaataagt tctttttcat
     2161 agagttcata tgattttttc cataccttag ctacattagg agatactacg tatccctctc
     2221 ttgcatagtt tatcgctgga gataagcatt ccattaagct aagtttacca tattttttat
     2281 ttaattctga ccaagctgct ggtattccag gtacagttac tgctccaaac ccatattttg
     2341 gcatttcttt catattatta taatttttta attccatatt ttctggagca aagccactag
     2401 aatttaaacc atacaatcgt ttttcttcct ctatccaaac aagggcatat gcgtctcctc
     2461 caatcccatt gcttgtaggt tctactactg ctaatgtggc ggctgttgca actgctgcat
     2521 caacagcatt tccacccttt tttagtattt caagtccagc ttgagatgct aatggtgttg
     2581 atgttgcaac tgctccattt ttagcataca cgacatttct tattgatgag tatttatgtt
     2641 ggtaggcatt aaacttcata aatttcagct cctcgtcatt ttttatttga tattctgctt
     2701 agatttatta tataacatca agtaatgtaa ctgatacaaa atgatataaa atatagactt
     2761 tttcctaact ttaattttgt ctttaaataa atatcttaaa ctcaattcta gtattttcta
     2821 tctgcactta aataagttat tttattcaaa acatatatct aattcttttg acttaaaaat
     2881 aatttattct tttatcatgt ttagagcata ttttaaagtt aaaaaataat atataaaata
     2941 tcctataaaa taaatgacta gagttatgat aattggtaca agtaatgacc ttggtataaa
     3001 cttttgtgct atagacatag caaataaatt atgaactgta cctacaacta atggcattaa
     3061 aaatataact tttaactgtt ttgatattat tttctcaata tctttattat ttgctccaag
     3121 ttttattaac ataatatatc tttccttatc atcatatata tttgataatt gtttaaataa
     3181 gattatacta cctgtacata aaagaaatac tacagacaaa aatatgccta taaacagcat
     3241 tagaccactt aattttatca agttattata actttcaata cttgatgtaa aattaaaagg
     3301 ataagtaaat ttcatatttt cacttactat attttttaat tttaaagtca aatccagttc
     3361 ttttttttca ttttttatat caattaatct tattaatcta gaatttccat gtaattttaa
     3421 ctgattaaat acgtcatctt ttgctacaat taaatcaaac gtagcttgtg aatttatttc
     3481 tggctccaag atgctttttt gtactttaaa cagatttata tcatactcat ttgtatcttt
     3541 aatatgcaag tttatttttt caacctcaaa gtctttaaaa aatatttttt tgtatgaatc
     3601 agaaacatag tacacagatt cctttgaagt taattcttca taattgttat tctgatattg
     3661 tcttaatttt ttaaaatcac tttctctaat aatatctaca gacgttctat cttctatata
     3721 actatttcca tttttataat acatttcaat atttatatct ttgtctataa gttctattgt
     3781 tttatcaaag attatcttat tatctttttt atatttatat aataatttgt caatttcgtt
     3841 atttacataa gaatttccta aatttatggt ataactgtat ttataatttt cacttatgtt
     3901 cctatctata tcgtaataaa gtgatatggt aaaccctagc atagttacac ttgtagctat
     3961 taatattgct attacagcca atacactact attgctcttt aatttaaatc caagttcaga
     4021 aaatgcaatt aaattctgtc ccttataata aaaacttttt ctcttcttaa cagcatcaat
     4081 aatcatatac atcgttgatg aaaaaaataa aaaagttcca ggtattattg taactaatat
     4141 aaaaactgaa agcataatat tttcagcaat aaaataacta attgacatta aataagaaat
     4201 agctattaat aaaacaccaa gtacacattt taactttata aattttttaa acttatactt
     4261 ttctggatat ttattaaata actctataat tctcttattt ctgataacta tagtatttct
     4321 tattccaata actatgaata ttattaaaaa taccgctagt gtctgaacta aggcctttat
     4381 acttaagttc atatttacta ctatcatttc attcattaat tttacaagca tcatagtcat
     4441 aagctttgaa aataacagtc ctaaaaatat acctattagt attgcaacta tacccatcaa
     4501 aaaagtttca tataaaaata tttttcctat ctgatttttc ctcattccaa gtaaactata
     4561 agttcctact tctcgcctac gtctattgaa aaaaaatgag tttgaataat atataaataa
     4621 aaatacaaat accactatta caattgaact tgtattaata ctcatcctag ttttctctcc
     4681 aagtgaactc aacgcttcat tatactctat agatttaaaa ctaaaaaata caaatacact
     4741 aaatacaatt gaaataaagt acattaaata attaaaaaaa ctattcttta cattttgcat
     4801 tgcaattcta aataaagtca tttttattaa ctcctccaat taaggaaact gtatttacta
     4861 ttttattata gaactctctg ttactttctc cctttacaat ttctgtgaat attattccat
     4921 cctttataaa gagtattcta ttgcaaaaac tcgcacttat agcatcatga gttaccatca
     4981 atattgtttt attgctgtct ttgtttaatt ttaatagaca attcaacaaa tccctagcag
     5041 atttagaatc taatgctcct gtaggctcat ctgcaagtac taatgatggt tttgttgcta
     5101 tagctctaca tacagctcct ctttgctttt gtccaccaga aacttcgcca gggtatttat
     5161 ctaatataga ttctatattt aattcttttg aaatctcttt tattctattt tctattactg
     5221 atactctttc attttttaaa gaaagaggca acattatatt ttcttttatt gtaagtgtat
     5281 ccaaaagatt agaatcttga aatacaaagc ctagattatc acgtctaaaa tctgccaatt
     5341 cattttgctt cattttaata gtattctttc catttatata aatatctcct gatgaaggta
     5401 aatctattgt agaaataata tttaaaagtg ttgatttacc agcaccggaa gaccccataa
     5461 taccaacaaa ttctccttta taaaccttga aagttattcc tcttagagct tcatgttttg
     5521 aaccttttct tccatactcc ttttttatat tttcaacaga taatatttct tccatagtta
     5581 ataatcacct ttcatcacaa ttagttattt atcaattctc acaaatatat tatatatatt
     5641 aaaatggctt ttaaacattt aattatatta atttaaggtt acattttcgt aagtattaat
     5701 tatatttctt caaatttatt gcaattatgt ttaaaatata atttatcaag taaaaatata
     5761 gttatattaa tataattatt ttaggtaata aggtagaaat attttaattt atcaagtaga
     5821 atatagttat gttaatataa ctattttgaa ttaggaggta agaatgttta aaataatggt
     5881 agttgaagat gacgtctcat tgaaaaatat catagcaaag tgtcttacta aatggggaca
     5941 tgatgttcat caaatagaaa atcttgaaaa tataatagaa gagttcaaaa actataaccc
     6001 agaattagtt ttattggaca taaaccttcc attttatgat ggttttcatt ggtgtaatga
     6061 aataagaaaa atttcaaaag ttcctataat ttttatttct tcaagaaatt ctaatatgga
     6121 tgtaataatg ggtgtaaatt taggtgcaga tgattatata caaaaacctt tttctgtaga
     6181 tgtcttagta gctaaagtta atgccctttt aagaagaact tataattttg ttgataataa
     6241 ctcaaatcag attattcaca atggtgttac tttagattta tcaactgcaa caataaacta
     6301 tgaagataat actattgagc taacaaaaaa tgaaatcaaa attcttcatg agcttatgaa
     6361 atataagggg cagatagtaa gtaggaataa actcatgaaa aaattatggg ataatgattg
     6421 gtttgtagat gataatacat taactgtcaa tgtaaataga atacgctcaa agttaaatga
     6481 aattggttta gaagatttta tagaaacaaa aagaggattg ggttatataa tcagttaaaa
     6541 caattctaag taggtgaatt tatgagcttt aaagattttc tcaaggataa actaggtttt
     6601 ttagtataca attttacttt cttaattttt acagtatcag ttattatatt ttctcctgta
     6661 gatgtttttc tgactgatac tattttatat atattagttg taaatgttgt gtttttattt
     6721 ttatatctat ttattagcta catcaagaag aacaaatttt tagataagat aaaaaatgat
     6781 acattcaaaa ttaatatgaa tgatatagaa ttagctagat gtaaaaatga agaaaaaata
     6841 tacttgaaca ttattaaaaa tcatcaatat caatgtaata acataataaa taataatatc
     6901 gagaaatttg aagaaaacaa agagatttta aatatgtggg tacatgatgt aaagatacct
     6961 atagctataa taaaactcat tcttgaacaa aatgaaaatt taattgatga aaaaatttca
     7021 gatgacatag atagtgaaat atttagaatt gaaaactccg ttgaaaaaat tttatattta
     7081 tcaagactcg aagattttca taaggatttt ttaattcaag aggtaaatat tgaaaaaatc
     7141 attagaaata ttataaaaaa atactcaa
//
LOCUS       DAYASV010000171         7156 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYASV010000171 DAYASV010000000
VERSION     DAYASV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.176"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            210..1034
                     /locus_tag="ACGI68_003432"
     CDS             210..1034
                     /locus_tag="ACGI68_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFH0139835.1"
                     /translation="MNWIFSLTPALLGITNTILGEIIFYLAYTSPAIVGLFFISTIYP
                     QSAKQDFIYRYLSFKEIGIKWILLTILFFALTFITSLIIGNYFNVKTSDIDWIHIAVF
                     RPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVRFGFIGSSIILGLVCGIWYLGSYF
                     TPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVYINTSRSILSAGLVHMMYYFFNIQ
                     LLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFKQKVNSEIEQIKSDDFEFELDW"
     gene            1212..2036
                     /locus_tag="ACGI68_003433"
     CDS             1212..2036
                     /locus_tag="ACGI68_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0139836.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2162..2743
                     /locus_tag="ACGI68_003434"
     CDS             2162..2743
                     /locus_tag="ACGI68_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139837.1"
                     /translation="MKADSTKEQLINATIQLLSSHRDVSSITAREIVAKANVNLAMIN
                     YYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFDFIGEHSQFI
                     KISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSFIQLLFLRSN
                     AFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2847..3023
                     /locus_tag="ACGI68_003435"
     CDS             2847..3023
                     /locus_tag="ACGI68_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139838.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4289..5041
                     /locus_tag="ACGI68_003436"
     CDS             4289..5041
                     /locus_tag="ACGI68_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139839.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5031..7142
                     /locus_tag="ACGI68_003437"
     CDS             5031..7142
                     /locus_tag="ACGI68_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139840.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNEEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2669 a          950 c          947 g         2590 t
ORIGIN      
        1 aaataattaa tcaaaatata aaaaatagga tattattata ggcaataact aacatcaagc
       61 gataacttaa tacatataaa aataaataaa caattaataa aaattaaaac ttcattataa
      121 aacggaagga tagtgaaaaa atgcaactat ataacacccc ctttaataca aacaaacact
      181 taatctattt ctttacattt actctagtat tgaactggat attcagtctt acacctgcgt
      241 tgctcggaat tacaaataca atacttggag aaatcatttt ttatcttgca tatacatcac
      301 cagctattgt aggtctattt tttatttcta caatctatcc acaaagtgca aaacaagatt
      361 ttatttatag atacctaagc tttaaagaaa tcggaataaa atggatacta ttaacaatat
      421 tattttttgc tttaactttt attacttcgt taataatcgg aaattacttt aatgtaaaaa
      481 cttctgatat agattggata cacatagcag tgtttagacc ttctaaaata gcttatatgc
      541 tattagcaag ttttatattg agtatactta gtcaagaagc tggatggaga ggatatgcca
      601 ttgacaaatt attagttaga tttggattta taggctcttc aattatttta gggcttgttt
      661 gtggaatctg gtatctaggg tcgtatttta cacctaatca aataccatat aatttggcac
      721 aatactccct atttgatgct tttttattta taccaaacat tgtactttta aactttataa
      781 ttaattttgt atatatcaat acaagtagaa gcattttatc agctggatta gttcatatga
      841 tgtactattt tttcaacatt caattacttc tacactaccc tattaaatta tgtgtaatag
      901 ctcaatatgt acaaataata tttggtttaa tatttcttgt atatatgatt tcttctaata
      961 aatttaaaca gaaggtaaat tctgaaattg aacagataaa gtcggatgat tttgaatttg
     1021 agttagactg gtaattttaa tatgaattat aaattaagat aatctatgat ttgatacgat
     1081 tactacttaa atataattta ctctctataa tttattgcaa catctggaaa ataattgtat
     1141 ttccacaaac gcttgaagta tataaaaaca aaatatatac tgattataag tgaaaaggag
     1201 gtcatatttt catgtttaaa ataggagagg tttcaaaact aacacaaatt tctattcgta
     1261 tgttgcgtta ttatgatgaa cttggtattt taaaacctgc aaaaacagac aaatatacag
     1321 gtcatagatt atactcagtt gaacagattt caatattaca aagaattgta ttattgagag
     1381 attcaaaatt tagcgttgca gaaatagcaa atatcgtaca taattggaat gatgaatttg
     1441 taattaagga attgaataga aaaaagaatg aaatacaaaa ggaaataaag caggaacaac
     1501 aaagaataaa taaaattgat aaatttatag aagctataaa ctgtgataaa gatgaaattc
     1561 attacaatgt tgtctttaaa aagattccaa gttataaaat catatcactt agagagataa
     1621 ttccagacta ccaatctgaa ggtatactct gggaaaagct atctaaattt atcaaagaag
     1681 aacatataga agtatcacgt caatccaaca ataacatagc tttttaccat gatgaagaag
     1741 tcaaagataa tggtgtagat attgaagttg gaatggttgt aaagaagatt ggaaagaaca
     1801 aatctggatt tatatatcgt gagacagaag aaattgatat gatggcatgt actatggttt
     1861 atggtccata tgaaaatata gctggtgcat atgaatcgtt ctgttattgg ttagataaaa
     1921 atagtgatta tcaaatatca ggtataaata gacaaatcgg acataaaggt gaacataatg
     1981 aaataaatcc tgaaaattat cttacagaaa ttcaaatccc ccttataaaa gtctaataga
     2041 ttagtactta aatacttaag tactaatcac ccattgacat cccaaaattt tcatgatagt
     2101 atctaaacgt gtattaaaca tgtttaattt ttttatggag aaacttttaa ggggaaaaat
     2161 tatgaaagca gattcaacaa aggaacaatt aattaatgcc acaatccagt tactatcttc
     2221 acatagagat gtttcaagca ttacggctag agaaattgta gctaaagcaa atgtcaattt
     2281 agctatgatt aattattatt ttaaatctaa agaggagtta attaatattt ctattaaaaa
     2341 atttttaaag cgtcatatgg atgaatataa ggctacacat gatactaaaa atttagcacc
     2401 taaacagcaa ttaagggaaa tactaattgc attttttgat tttataggtg aacatagtca
     2461 atttataaag atttctgtac cctatatatt gatacaggaa gaaattgttt atccatttga
     2521 gatactacct ttaattaagg ctcactatga aaatcagaat aacaaaaaaa ctgaagctga
     2581 atacagaact attgcgtatc aaataacttc atttattcag ttgctttttc ttcgttctaa
     2641 tgcttttttc aagtatagtg gtattgatat tcttaatatt gaacagagaa atggtttaat
     2701 agattttcaa ttagatttat tattggtaga tattacaaaa taatactata ttaatttttt
     2761 atgtacattt ttttatttta tggtaacgtt tgaaaatatt ctcattgact aaaaatactt
     2821 gcattaaata agggggagga attttaatgt tttttacatt tataggagta atatcagcaa
     2881 ttttattgtt gtgtacaatt atatgtggtt tatggataaa acttaataat cgtaataatg
     2941 actctaattc tcactttact ttttcaatac tttcaattgc tttttcactg ttaattgtgg
     3001 tggccttttt agtaagaaat taatgtcttt gttatgctga ggtttattat tatagatttt
     3061 tattattgca tgatttttac tatattatag tatttttatt atattacagg gtcttattta
     3121 ttatagtgtt ttgttgttac agcattttat tatattacag agtcctattt attataggat
     3181 ttttattata ttgtaaaatc ttatttatta taggattttg ttatattaca gacttttatt
     3241 atattgaaat ctcttattta ttgcagtgtt tctattatat tgcaaactct tatttattat
     3301 ggtgttttgt tattgcaaca tttctattat attgtaaagt cttatttatt acagttcttt
     3361 attatactac agacctttat tattacagca tttctattac attgtaaagt cttatttatt
     3421 acagttcttt attacactac agacttttat tattacaatc tcttatttat tacagttctt
     3481 tgctatacta cagaccttta ttattacagc atttctatta cattgcaaag tcttatttat
     3541 tacagttctt tattatacta cagactttta ttattacagc atttctatta cattgcaaag
     3601 tcttatttat tacagttctt tattatacta caaaccttta ttattacaac attttattat
     3661 tttaacttct ttactataat atagtaatat tttaatatct caaaatcata tcaatcactt
     3721 ttcagcatag tctaaaatac ttccactttc taaaattatc tatcatacct atactaataa
     3781 tactttttaa cctttttatt atacatttca attgtttcta acaacctatt tatttccaaa
     3841 actccaatat attttctcaa ttacattgtg ttaatagccc ctaaatcctt tcaaattatt
     3901 tacaatacat tccattaaat aatcttactg ctctataatt ctttttttct ttttaaccct
     3961 actatacatt atttacatca tttttttcaa tacaacattc gttattctat aaccttttta
     4021 cttttactat atacttcttt tctaattaca ccttcaatgt tccacatcat attactttag
     4081 cacaacaatt aatacctaaa aataaccctc taaatcctcc aataatataa tcaatttaac
     4141 tataataata acctctcaca ctatccaaac aatatatttt caaacattac aaaaaaacat
     4201 ccaactatta caatatagct catatccctc ctcccctttc aaatgctatc attgacttac
     4261 aagataaatg aaagtgagga tgatatatat gcttaaagta aaccaattat gtaaatcata
     4321 taaaacaggc agtaaaacat atcctgtatt aaaaaatatt tcttttgaaa tcaacaaagg
     4381 agaatttgtc gcaatcatgg gttcatcagg aagtggaaaa actactcttt taaattgtat
     4441 ttcatgtttt atcccaattg ataatgggga aataattctt ggaaataata atataagaaa
     4501 tttaaaagaa aaggaacttt cagaagttag aaatcaacaa ctaggatttg tattccaaga
     4561 cttcatgcta tttgatggtc ttagtgtctt tgaaaatata tgtgttccac aaattatcaa
     4621 aggtagtcaa gtcttacaaa tggaaaaccg tgcaattaat ctatgtgagc tatttggtat
     4681 tgaacatatc agaaaaaaat accctgccga aatatctggt ggagaaaaac agcgtacagc
     4741 agtagcaaga gcactcatga acaacccaga tttaatatta gcagatgaac caactggtaa
     4801 ccttgatagt aagtcttgtc gtacagtcat tgaatcattt ttaaaagcaa agcaagaatt
     4861 agatgctact atactcatgg ttacacacga tagctttgca gcaagcttct gtgaccgagt
     4921 tattgtgctt aaagatggaa taatttataa agaagttgta aaatcaaatt caagacataa
     4981 attcctagat gaattactag aagttttaaa agaaatggga ggcgctcaag atgacaatta
     5041 ataaattata cagtaaactt cgagcctata attggaagaa ctatacaact ttattctttt
     5101 gtataatact ttctgtggta ttagtaactt catatgctct aatttatttt agcccaacaa
     5161 tacaaaacat acttccaact ggtggagata gcagaaaaca ggctacaatg atattctcag
     5221 ttgctatatt aggatgcgct gtctttacta catacgcttc ttccctcttt ttaaaattta
     5281 aaagcaagga aattggtatt ttcatggctc taggaacaga taagaagaaa ttaaaaaaac
     5341 ttctattttc agagatattg ttaatttcaa tagtatgctg ttttttaggt cttatattaa
     5401 gtattcctgt atcttttggt atatggaaaa tatttcaatt agtgattatt gataccagag
     5461 aaatgacata tcattttgga actcaaggat tcatatatgg atttatattt agtgtatttg
     5521 tgacattatg catatttata ctttctgtaa aatttattaa tcgtacaaat ataattgata
     5581 tattaaatga ccagagaata tctgaaccaa tacaggatgt aaaggactgg tatggtatat
     5641 taggtataat ttttattata ctcggtattt ttctaggata tctcgtacct tcactaacta
     5701 taaacaaatt aaattataaa ctaccatcta tatggagctt tacttatcta ctctctctta
     5761 ttggaatata tatgattatg acatacatag ttgtacatgc taaaagagga aaacatccag
     5821 agaaatacta taaaaacatc atatcaacta gcatgatgag atttatggga agacaaacag
     5881 taaaaaacat gtgtgttatt tcatttttga ttgcaggagc attatttgct gtattctatg
     5941 ttcctaatat ggttacaggt atctatgata ctcttaaaaa taaccctata gattatactt
     6001 ttacctacaa tcaaagagat aaacaggtat caaaaaaaga tattgaacaa ttagcagata
     6061 aacacaatat aaaaattcaa aactatatgg aaattccttc tatagaatta attgttgatg
     6121 gtgtagaatc aatatatcat aaagatggaa ctatcgaaaa ggtatatatg aaaaaaagct
     6181 cctatgatat gttctttaat tccaaagatt tttccaagct aattggcaaa ccaataacta
     6241 taaaaaatgg tgaatatctt gctgttgtaa ataaaggaaa tgaaaatcaa gattataaat
     6301 ataccaaatt tatatcaagc ccagtcacag aaaaagaaat cccctttaga tatgctggaa
     6361 aaataatatt taatggttct ttttttcaaa atagaagtat gaacgcctat gtccttaatg
     6421 aggaggatta taaatcattt actaaagatt tatccataaa aaattacaat aattctatac
     6481 tatttaatgt agataaagat acctatgctt ttgccaatga cttaaagaaa gaaattataa
     6541 aaagaacatc taaggaaatg ggcatgttca gtggttatga tgaatacgta aaaaaacgtt
     6601 atgaatcaac tggaagaaaa tattttatgg atgaagaata tccaagtggt gaaggtcatt
     6661 taaaactaga cccaaacaac aatcagttgt atcttaattg gaaatactat cctaacttta
     6721 aaattttaca aagccaagat atgataaaaa acatggcagt cttcttaatg ttattcatat
     6781 atgtggcaat catatgtttt acagcagtag gaatcattgc atatacaagg ggtgttacta
     6841 tagctataaa tcacaagcag gtctttatgg atttaaatcg attaggagca aacaatgatt
     6901 atataaagtt ctgtattaga aatcaattaa agaaagtatt ttcataccct cttattgcag
     6961 gaagtttttt aatttatctg tttaatttta tgattatgtt aaataatgat ggtagaatcg
     7021 tcaatagtga ggtcaatgct cttttgataa acttaggttt tatagggatt tgtggggttt
     7081 atatgtacct tatatatcta ttaacttata agaaattcaa aaaaattatt ggtattttat
     7141 aataaaaaag gttatt
//
LOCUS       DAYASV010000172         7104 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYASV010000172 DAYASV010000000
VERSION     DAYASV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.177"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            95..1366
                     /gene="sleC"
                     /locus_tag="ACGI68_003438"
     CDS             95..1366
                     /gene="sleC"
                     /locus_tag="ACGI68_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic germination protein SleC"
                     /protein_id="HFH0139841.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(1792..2448)
                     /locus_tag="ACGI68_003439"
     CDS             complement(1792..2448)
                     /locus_tag="ACGI68_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139842.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGVRILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(3560..3970)
                     /locus_tag="ACGI68_003440"
     CDS             complement(3560..3970)
                     /locus_tag="ACGI68_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139843.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKFESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(4186..5568)
                     /locus_tag="ACGI68_003441"
     CDS             complement(4186..5568)
                     /locus_tag="ACGI68_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HFH0139844.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNSKDVLYTDSGEVSIERSGYNDNRFVILRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(5579..5938)
                     /locus_tag="ACGI68_003442"
     CDS             complement(5579..5938)
                     /locus_tag="ACGI68_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0139845.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(6143..6922)
                     /locus_tag="ACGI68_003443"
     CDS             complement(6143..6922)
                     /locus_tag="ACGI68_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFH0139846.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         2506 a         1204 c          743 g         2651 t
ORIGIN      
        1 ttaaatttat atctatatta tttaacaaat ttcaagctat attcatatct tgaaatataa
       61 gggaactaaa ttcatttaaa gaaagggtga tttgatgcaa gatggtttct taacagtaag
      121 cataattgat gctactaaca atagacctat tcaaaatgca gtagtaaaca tatattcaat
      181 gtctaatggt agccaaagct catcaacttt ataccaaaac ctaagaagta atgaatctgg
      241 tcaagtgact ggtttagtat taccagcacc agatgtagat tactctctac aaccatctga
      301 tgttagacct tatagccagt atatagtgga agccatagca gatggatatg agacagttgt
      361 catagaagga acacagttgt tagctacaat tgaagcaaga caaggagttc ccatgtctcc
      421 tagaacaaga agtaagcgtt ctttctcaag acaaagtgag ttgatttttg atataggaga
      481 acatactcta tatggtactt atcctcctaa gattccagaa agtaacttaa aaccattacc
      541 acctcctact ggtttcgttg tattagataa ccccgtagtg cctgaattta tagtagttca
      601 tgatggtttg ccagaagatt catcagctcc aaactattgg attccattta aagagtacat
      661 taaaaacata gcgtcatctg aaatatattc aacttggcct gaacagacaa tctatgcaaa
      721 tgtaatagct ataatatcat ttactttaaa tagagtcttt actgaatggt atagaaataa
      781 aggatataac tttacaataa cttcaacaac agcatacgac cataagttta taaataatag
      841 aaacttattt gaaccaatta acgtagttgt agatgctatt tttaatactt ttataaaaag
      901 acctcctaca tcaagacagc cactattagc acaatattgt gatggacaaa aatcccaatg
      961 tcctgaccaa atgactcaat ggggcagtaa agacttaggt gaccaaggat atgactatga
     1021 gagtatacta agatatttct atggagatga gatagtattt gaaagagcac ctatagtaag
     1081 tggtgtacca gtgtcattcc ctggtactac actacaagtt ggttcttctg ggcagtatgt
     1141 tagaaccata caaaatcagt taaatgctat atctaattct tatccagctg taccaaaagt
     1201 tatagaagat ggtatctatg gaacagatac agaaaacgct gttaagatat tccaaggaat
     1261 ctttggttta cctcaaagcg gagtagtaga ttttaaaaca tggtatgaaa tatccagagt
     1321 ttatgtagct acaactagaa tagcttcttt aaatccttta atttaaagct tgatttagaa
     1381 cataaactca aattattgaa taaaattatt aatttaaaat aaaagtggct gttatgaact
     1441 aatatatacc ataagtatta cttataagta ttgtagtctt aacagccatt ttttagttat
     1501 aacttgtttt tttagaatct taaaacttta aatgggggat tttaagcttc ttaaaaacag
     1561 tgcttagcaa cttagatttc tttaaaatat tatatttatt tttaagttta ataaaatgct
     1621 attctaacga aaaacctact ttttatttaa aatctcactt attatttaaa acactatcca
     1681 tcacttagaa tattacttct tatataaatt attatttatt ataaatacaa ataatattaa
     1741 tttaaaaagt tattcctaat ttaaataata ctactatctt catcagtgaa cctaaactgc
     1801 aactttagac ttattagcta aagccaatat tagacctaaa gctatagatg caaatgcaga
     1861 agcaattact ctatactcag cagggaataa aaatacaatt gtatgacatg gtatccagaa
     1921 aaatggtata gtttttaaaa cactaaaacc aaggtaagca ttccagtcca caatatttac
     1981 cacatcttta agttttacct taatcccctt tccaacctca tattttttat ctataaaaca
     2041 gtccataacc ttatggaata aaaatacagt tggtgacatt agtatattca tagttgcact
     2101 cttaaataga gcatttgcaa aagctattcc ctcaaatgga agcataccat ccacttgtgc
     2161 ttgacctaca cctgatgagt agattgacat tccatatgtg gtaactacac ctattattcc
     2221 ccaaactatt gctctataaa atactcctac tggtattttc caatccttat acaaaattct
     2281 aacaccaagt aaatctccca ttgtagctaa tacagcaaac tttaaaaaac ctccaatata
     2341 tggatgtgca cctgttgctt ccataaatac ttctctacta cttggtattg ctaagacagc
     2401 aataatacaa ataagaactc cactccatat aaaatcgcct tttttcatat ttataaatac
     2461 tatttaatat ataaatagtt ctcctttcta ttaaagattt atttaattta caatctatct
     2521 acaattattt attaatcact tattattaga tattcatttt ccatatttaa atttattcta
     2581 tccctttatc tatttatttt cttactactc tatccaccat tatctatata tttattttac
     2641 gattactctt tcaccctaac cctttaacta tctatttttt cttactattc ttatccacat
     2701 catccatata tttattttac gattactctt tcactctaac cctttaacta tctatttttt
     2761 ctgatattct tatccaccat catccatata tttattttac gattactctt tcaccctaac
     2821 cctttaacta tctatttttt tctggtattc ttatctacta ttatctgtat atttatttta
     2881 tgattactct tttaacctac cccctatact ttatagtaat atgtttttaa cctttttcat
     2941 tattcccaat atgctttata actacaaata ctctcccccc ttaaaaataa taataaaatt
     3001 aatcaataaa tttataatca tattattact ctatgtatta agcaattact atgccaataa
     3061 actttgattt ttttaaacaa atcttaaaaa tcctaaaatg ctaattttct ttaactttta
     3121 atgaatctaa tagataacat ttaattttcg ttatattttt cagaatattt ctattattat
     3181 ttttaagaaa aaaaaataat gttaacattt cttaacatac attaaaaaat gttaacatta
     3241 taaattgggt ttaatacttt tactttgact cgatttactt ttattctact tcttacttta
     3301 tttttaactt agactttatt tttttattac ttatttgagt cttatttatt ttattctcac
     3361 tccatatgtt caattacatt tgtctaaatt ctttgatact tttattacat aattttaaca
     3421 agcttaagtg ttatttgggt agtgcagttg ataattattt aaataatcat caactgcatg
     3481 tttatatcat tacagtagct taatgtattt aatcatactt acttaggatt ttgaaatatc
     3541 ttagagatta tatttaacat tagtcaattg ctgcataatt gtaagcaatt gaaatttgag
     3601 acccatcttc attttctata gtgataggct tcataatttt taatccttca gtttttatct
     3661 tttctaattc ttgttccatt ttcttaattg tttcctcaag gtctttttga cttacatctt
     3721 tgactgcatt agggtcttta gcatagtctt ttaattcttg aatatgttta gcatactctt
     3781 cataagtata atgctcaaca ccatactttt ccaattcagt agggtcagtt attcctgatt
     3841 ttattagaga ttcaaattta ggtaaagcaa tattagatga atcatttcca tttgcaaata
     3901 ctgctccagt tcctcctagt accattgcag ataccatcat tgcacttata agggattttt
     3961 ttattttcat aaccaatctt cctttcttag agatttattt gggtagtgta gtttaaaatg
     4021 actattgcaa tagttatcaa ctacactctt agactatcac agtagtcatg tttatgtcaa
     4081 gcctttttaa aaaacttttc aataaaaaat actatctcaa agcaaaatta attattttga
     4141 gatagtattt taattctaaa tcttttatat tactaagatt tatggttatt ctatccatgt
     4201 tctatattgt tctttattaa ctgctaacaa attccctttt cttaatatga caaagcgatt
     4261 atcattatag ccacttcgct caattgaaac ttctcctgaa tcagtataaa gtacatcctt
     4321 actattacta gatttattta catacgtttc ttcactattc tcagaattag ttactttgct
     4381 catagatgat tcttcatgct ctctaaattc aattacagat acttttgccg tactaaaatg
     4441 agtctctcca tcacttttag ctttactaac aagaatatca gattctactt tgggagaata
     4501 taaatcagtt gatttactac ttattgaaaa tccaatttta tcactttgtt cattttttgt
     4561 taaaccaaaa gattcaacct cattaatctc tttattttct gttgcaaaaa caagacctgt
     4621 cccacaaact aatacagttg aaactaaaaa agtaacataa gttgattttt taaatttcat
     4681 aatattaact attctctttt ctatttttgt ctttccaaat ccactacata ttgtcattga
     4741 aaatggatta ttctcctctg ctagacttat tagtgtggtt gcataatcag ccctatattc
     4801 ttcaccaagt atactcataa ccttttcatc acaactaaat tctatgtctt tatttagtaa
     4861 aaaaaacatc acccaaacta agggattaaa ccaatggaca acaagagcta caagcataaa
     4921 taactttaac agattatcaa agctattaat atgaaccatt tcatgagtta aaacaaagtc
     4981 taaagtttga ttattagcat aattcatttg ctttggcaaa ataattcgtg gttttaaaat
     5041 accagatgta agtggtgtcc tagtaaattc agatatgcaa acaataaatc ttctaaaacg
     5101 gctattctta cacatccatt ttttaatgaa ctcatgattt tttatatgaa ttgaagtttt
     5161 aaaaagatta attattttaa aatatctaaa gcataaaagt atgctaaaca taatagtaac
     5221 tataaaagac aaaatgccca acatttccaa catcataata ttttcaatat tagacattat
     5281 ttcaaattcg tcagaattgt ttttacctgt agagtcacta gctttcttaa gagtataatt
     5341 ttctgttgat gtattaccca attccacatc aaaagtcgat tcaattatac tataaatact
     5401 aaaaccagaa tttaaactta ctggtattag aagtttaaat aaaacaatca accacaataa
     5461 tacaaatacc tttttaggta attttttaaa taaacatgct cttataaaaa taactattaa
     5521 aagtaatact ccagaagtaa aactcatttc taacaattta atgaacataa atcttacctc
     5581 actttaattt ttcaacaatt tgtttaagtt ttgtaatctc atcacttgaa agattttctt
     5641 tattaataaa agctgaaaga aatttttctg aagaccctcc aaacatttta ttaattaaag
     5701 tttctgtttc ctgtttttgt acttcctctt tgcttattaa agctttacaa acaaaattcg
     5761 gctcaattct ctctattgca ccttttgaca ctaatttttt tataacggta tacgtagtgt
     5821 ttctattcca tcctgtttct tcttttaaaa tttttactaa ctctccagcc ttgctttctc
     5881 ctctctccca tagaacttcc attactttaa gttctgaatc aaataatttt atctccatat
     5941 aaaatcgctc ctcaaatata gttatcacaa tagtcattac tataataaaa aactatcata
     6001 gtagtctata ctatgtcaat catttatttt ttaataataa aaactgtctt tttaaaaaat
     6061 tcttataaaa gacagtttta tcaatgttat actttaatgt ttaattaaag ccatatttta
     6121 tttaaatata taactatcta tcttagtaca tagtataaga tttttcttta tctaatagta
     6181 tatcctctac atctgggttt tcttttacta attgtctata aaaattacaa ttagtaacac
     6241 ccacatatgg aattagccat agtagaccta ttccaagagt agctattgaa agtatatacc
     6301 atcctataaa gctaaagttt agtacaaata atttaagtac atttcctttc atcatatcta
     6361 aactagcacc cattgattta aatacaccca tattatcctt gtcttcacaa attaagaaag
     6421 tagcttgaga aacatataat agcaatatat aaactggtaa cataagaaca agtattataa
     6481 gaactagagt tattccagta gaaataatag tccctattcc aacattatca cttgataaat
     6541 attcaattgc tccactagta agtaaaactc caaatgataa aaacgctcct ataattgtga
     6601 ttataaaaga ataaatatac acaataattg attcaaataa ataaaaacca aaacttctta
     6661 aataaattct cccacttaca aagatatctc ctatcttgac tttttcatct ttagtaattt
     6721 tcaagaacat acatgtaaat cctgctgaaa cgcaaaaagc tattacagcc actacaatag
     6781 tagtcaatgg ttcaaaaggt aatcctacaa ttgatattaa tactatcaat gcaatatata
     6841 ctgccataga aattgcacat atcccccagt ttccagataa ctgagcttta gatactcgtt
     6901 ttaattcttg ccttgatacc ataaaaaaca cccctttcat tttataaaaa aacttttaaa
     6961 aatataaata atcttttaac gtataatatt aatatatcat attttcgact atataagata
     7021 caattattca ccatgtttta tcaagatatg ccataattgt attactagtt ttatatagtc
     7081 aacattacca aatacatcaa ataa
//
LOCUS       DAYASV010000173         6917 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYASV010000173 DAYASV010000000
VERSION     DAYASV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.178"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            62..1318
                     /locus_tag="ACGI68_003444"
     CDS             62..1318
                     /locus_tag="ACGI68_003444"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0139847.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            1341..3422
                     /locus_tag="ACGI68_003445"
     CDS             1341..3422
                     /locus_tag="ACGI68_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFH0139848.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSTKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIIVMIL
                     SMLYIKKYKRR"
     gene            3459..4544
                     /locus_tag="ACGI68_003446"
     CDS             3459..4544
                     /locus_tag="ACGI68_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0139849.1"
                     /translation="MRKYMNEFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGLVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            4598..5722
                     /gene="wecB"
                     /locus_tag="ACGI68_003447"
     CDS             4598..5722
                     /gene="wecB"
                     /locus_tag="ACGI68_003447"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFH0139850.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            5800..6774
                     /locus_tag="ACGI68_003448"
     CDS             5800..6774
                     /locus_tag="ACGI68_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFH0139851.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNGKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         2881 a          644 c         1151 g         2241 t
ORIGIN      
        1 atttttaaaa taaaggattt tatattagaa tcaatatttt aaaatatttt aggagaaatt
       61 tatgagagat ttaaatgtat cagagtattt gttcgtattt agtttgttta gtatatggtc
      121 tttattgctt ataaatatta ttttagctat gggtggatat atattttatt ttaaaaactt
      181 tgataaagaa ataaaagaaa ttgatgaata tcctatgata tcaatacttg ttcctgctca
      241 taatgaagct aaagtgatag gtagaactgt ggagtctttg ctattactca attatcctaa
      301 gagtaagatg gaacttattg ttataaatga caattcttct gataatagta aagaaatttt
      361 agaaaatata aaggataggt acaacaatta taatttcact ataataaata cagatagttt
      421 aacaggtgga aaaggaaagt ctaatgcatt aaatattgga tatacgattt caaaaggaga
      481 ttttatagct gtttatgatg cagataacac accagataaa aatgcactta gatatctagt
      541 acaaactata gtaatgaatg atgaattagg tgctgttatt gggaaattta ggactcgaaa
      601 taaaaataaa aacctattaa ccaaatttat aaatatagaa acattaagct ttcaatggat
      661 gtcacaagct ggtagatggc aactttttaa tctatgtaca ataccaggaa ctaactttat
      721 attgaggaga agtatcatag aagaaattgg aggatgggat agtaaagcaa ttgcagaaga
      781 tacagaaata agttttagga tatataaatt gggatacaag ataaagcttg taccacaatc
      841 tataacatgg gaacaagagc cagaaactgt taaggtgtgg ataaagcaac gtactagatg
      901 ggcaaaaggg aatatatatg tattaatgaa atatataaaa aatatattta aacagggaag
      961 aaataagatt gtgtttgata tagcttactt tttttctgta tattttctat ttttaacatc
     1021 tgtaattata tccgatattt tatttgtttt aagtatatct aaactagtag aaatttctat
     1081 tccaattaac ttttttctaa tatggatact atcttatctt ttatttatta tagaagtgag
     1141 tataagcctt actatagaaa aaggtgaagc aacaattgaa aatatattca tagtggcaat
     1201 aatgtatttt acatactctc aactatggct gtttgtagct ataaaaggaa tgatagagta
     1261 tctaaaagat ataattttta aaagagaggt taaatggtat aaaacagaaa gattttgatt
     1321 catgtacagg aggaattaac atgaaaaaat ttattatatc aattatatct ttagtattgt
     1381 ttttttcaaa tatatcactt atatacaaag taaatgctga tgagactaaa gttaaaaatt
     1441 ataagtttga aagagatata actattgatg gtgttatagg aagtaatagt actttttttg
     1501 aagtaaataa aaattgggat attgaagaag ttttattgca tcttaatttt agtaagagcc
     1561 aaatattaaa tggagatgtt tcaagtttaa ctgtattgat aaataatgta ccaataaaat
     1621 ctattaaatt aaatgctaag acaaattata aaaatacatt agaagtatta gttccaaaag
     1681 attatataat tcaaggatat aatgaaataa aaataaaaac atacaaaaca atatctgata
     1741 aaatttgcca agatgattcc aatactggga actggatggt tattcacaaa gaatcatata
     1801 tatcaattag atataagcag aaaaaggtgg aaaactcaat aaatgaatat ccgtatccat
     1861 atgcagaaat tgaaaataat cataaattag atacaactat agtagtacca gataatatga
     1921 ctagaggaga gactacagca gtgtttaatt tggcatcagc atttggaaaa ataacaaaaa
     1981 atgatgattt aaagttagat gttaaattat attcagaaat gaaaaattgg tcagatgata
     2041 atattatata tattggtaaa cctgaaaata cagcagaaga aattttggat attttaagta
     2101 taaaggaaca aaccctatta tcttcaaact gtataatcaa gcaagtagat tcaccataca
     2161 ataagaataa gaagatgatg gtggtaattg gtagtaatga agatgattta ataaaggcat
     2221 caaatttatt aatagaaaat agactttcta accaagtttt atcatcatca gtacttgtaa
     2281 acaaagagac taatataaaa attaataggg aacaaaaatt aaatttaggt catttaacat
     2341 taaaagattt aggatattct gactttttgt tggaaggagc atttaatcaa caagctttgt
     2401 ttgatgtcaa aataccaacg ggtaaggtat tagatgatgg ttcaaagatt atcttaaatt
     2461 taagatattc tgacaattta gattttgaaa aatctttagt tacagtgtct ataaatgatg
     2521 ttattgtggg aagtaaaaaa ttggataggt cacattctaa taatgataag ttagaattaa
     2581 aaatacctaa agatatagat aataaaaact attatcaagt taaattgaca tttaatttga
     2641 gtataaaaaa ttcgaattgt gttactagag agagtaataa tccttgggca tatgtatcaa
     2701 ataattctta tttggcgtta tctacgaaag aaaatgaaac cttatctttt gaaaattatc
     2761 cgtatccatt tgttagagat gatgaattta atgatttaac tgtgataatg ccagattatt
     2821 cagggtcaca agctatgacc tggatgttta gattaggtgt tactttaggt gcgaatataa
     2881 attcacataa tggaaatata aatgtaatta gaggaaaaga atttagtgac aagtataagg
     2941 atactaatat tgtagttttt ggagtgccac ataataattc tgttataaag atgttaaata
     3001 ataatttaaa tataaagttc gataaaaatt actccaattt tatatcaaat gataagataa
     3061 gctttattga tgattatgga aaaaatattt ctactattca attaataaaa tcaccatata
     3121 ataatcaaaa aaatatcatg gtaattagtt caatgaatga aaaaaactta tatttaggaa
     3181 tggattattt gttgaataaa agtaaagtta atgatttaaa aggagataca ttgataattg
     3241 atgaatacgg agaggtagaa gatttagctt ataatttaaa atctaagaaa gaagtaaaag
     3301 attcaagttg gaatatgagt ataaataaga caactaaagt atttttaatg atttctttta
     3361 ttactataat tatagtcatg atattatcaa tgctatatat aaaaaaatat aaaagaagat
     3421 aatatgataa atttattatt ttaaaagagg tatataattt gaggaaatat atgaatgaat
     3481 ttaaattaaa aagaagtatt ttaatctcat taataatctt aatttttata tttatagttt
     3541 actttgtaag tattgagtta aaagatagga gtctagtaaa taatcaatat aaaggtaaga
     3601 ttaaatcggc caatttatct gtagattatg aaataaatca agttatgaag gatatagata
     3661 aattgggatt gaatacagta aatgtaccta taattataaa tgtagagtcc attaattctg
     3721 atattatgtc aatagataat aatagtaaag aaaaggcaat taagttaata aaaaaattaa
     3781 ataaaaaagg tatttcaaca atattagagg catatccatg gattgaaaat ggaaaattgt
     3841 atgagactga ctggaaccct ataaataaaa aaagattttt ttatacttgg caaaatgcca
     3901 tattaaatga acttataatt gatgtagcaa atccactgga tgtaaatgtt ttaaatattg
     3961 gttctaattt tgtacatttg gaagaatatc aacaaaattg gggtgaaata atagattttg
     4021 tacaatccaa atttgacggt cttgtaacgt acagaactaa ttggtggtat acaaaaaaag
     4081 atgatttaag tagtaagttg ttttatgaac aaaagctgaa caataatttt tttgataagt
     4141 tagattttat atctatagca gcatattttg agttaagtaa taaacctgtt aatacagtgg
     4201 atgaattaat ttctgcctta cactcatcta ctgtaaacaa tagaggacaa aatataaaac
     4261 aagaaatata taacctttat aaagctcata gaaaacctat attttttgga gaattaggtt
     4321 tttcaaatag agaaagtgca agttctcagc cgtggaatca tactccttca aaagctgtaa
     4381 atggcgaaga acaagcaagg tgttttgagg catataagaa agtttttgaa aatgaagact
     4441 ggataaatgg tttttctgtg ttttgtgtag gtaaaataga tgacgaaaaa aatttttacc
     4501 catcaaaaga gagtataaaa gttattaagt cttggtatga ataattttga aagtgtagaa
     4561 tttatataga ttaaatagat gattaggaga gatataaatg aatgacatca aggttatgac
     4621 tgtatttgga actagacctg aagctatcaa agtagcacct ttaataaagg aacttgaaaa
     4681 aagagagaat ataaaaagta ttgtttgcgt tacagctcaa catagagaaa tgcttgacca
     4741 agttatagaa acctttaata taaatgtaga ctatgatttg gatataatgg aaaaaggaca
     4801 aagcctgaat gatataacat gtaagatttt aaataaactt cccttgattt taaacaagga
     4861 aaatccaaat ataatattgg tacatggaga tacaaccaca actctagcta caagtttgac
     4921 tgcattttat aataaaactc ttgttggtca tatagaagct gggcttagaa cctatgataa
     4981 atattctcct tttccagagg agctaaatag acaacttaca ggtattattg ctgatatgca
     5041 ctttgctcct actaatttgg ctagaaaaaa cttgatttct gaaggaaaac ctaataataa
     5101 tatatttgta actggtaata ctgctattga tgctttaaag atgacaataa aagaaaacta
     5161 taatcatcct ataattgatg agattggtaa cgataggatg attttgttaa cctctcacag
     5221 aagagaaaat ttgggtaaac ctatgaaaaa tatattcaga gctataaagc gaatagtaga
     5281 tgactttgaa gatgtacaga tagtatatcc catacattta aatcctaaaa tacgaactat
     5341 agctgatgag atatttggaa aatttcctga aaaaattcat attatagagc ctttagatgt
     5401 agctgatttt cataactttt taaataaatc ttatatgata atgactgata gtggaggaat
     5461 acaggaagaa gctccatcat taggtaagcc agttcttgtg ttgcgtgata aaacagaaag
     5521 aacagaagga attgaagcaa agacacttaa attagtaggt actaatgaag atagaatata
     5581 taattcagtg agcgatttat taataaataa agataactat gttcagatga gtaaagcatc
     5641 taatccatat ggagatggaa atgcaagcaa atatatagtg gatataataa ttaaaaaatt
     5701 taattgcaaa tatttaaatt agataaggcc taaaacatta actaattgat ataagattta
     5761 tgataatatt aaggaataca atttttaagg gggagtaata tggctagaaa attaataaaa
     5821 aatttgggta aaagtaaaag tgtaaagaga gtgaaacttt tatttaagaa gatttttatt
     5881 actgtattca tagtagcaag tatagttgct atttttaata taacgaaata ttttgaggaa
     5941 ttatacaaag taagggattt aaaaagtact aaaattgaat actatatgga tgtggctgat
     6001 gaagcaggag atggaaaggt ccaattaagt tggaaggcct tacttgctat agacatggta
     6061 atacatgatg aagatttaag taatataaaa aagaaagaca cattggatat aggggaaaag
     6121 tttatagtag aagataaaaa tggtaaaggc gaaaaggtgt ataaggtaaa aaagtttaat
     6181 aaggtattaa gcgaattgaa atttgactct tctcaaaaaa gtagagcaag aaaatacatg
     6241 aaagatttag aatacacata ccttggaaat aaacaattag atagtagtga tgaaaaaatt
     6301 aaatttataa agaagttaga agactcagct ataagagaat atattgatta tggaatattg
     6361 ccctctataa caattggaca agctatatta gaatctggtt ggggaaattc taaacttaca
     6421 aaacagagta ataatttatt tggtataaaa gcagataaag catggaaagg aaaaagtgta
     6481 gaaatttcaa cttcagagca ttataatgaa aaaattgtag ctagttttag gtcgtataat
     6541 tcattacaag attctgtcaa agaccacagt ttatttttaa ttaataataa aagatataga
     6601 aaacatgggt tgtttgaggc aaaagattat attagtcaag ctcaagcatt agaaaatgca
     6661 ggatatagta cagcagaaga taaaaaagga aatcgtatat atgcagagct attaatagat
     6721 gttattagga gttataattt gcaattgata gataataaag ttgagacaaa ataattgtta
     6781 actttatatg aggagataac aagataagat ttttttctac aaaaagaata gaaaaattgt
     6841 aaaaaaaact aaaaaaatgt attgacttgt tatttaaaag gtgatattat tattactgta
     6901 ctcgaaagag aacaaaa
//
LOCUS       DAYASV010000174         6880 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYASV010000174 DAYASV010000000
VERSION     DAYASV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.179"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            168..2213
                     /locus_tag="ACGI68_003449"
     CDS             168..2213
                     /locus_tag="ACGI68_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0139852.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            2474..3673
                     /locus_tag="ACGI68_003450"
     CDS             2474..3673
                     /locus_tag="ACGI68_003450"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HFH0139853.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            3725..4420
                     /locus_tag="ACGI68_003451"
     CDS             3725..4420
                     /locus_tag="ACGI68_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HFH0139854.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            4433..5110
                     /locus_tag="ACGI68_003452"
     CDS             4433..5110
                     /locus_tag="ACGI68_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139855.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            5221..6822
                     /locus_tag="ACGI68_003453"
     CDS             5221..6822
                     /locus_tag="ACGI68_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139856.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCYGKIITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYPRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
BASE COUNT         2759 a          684 c         1301 g         2136 t
ORIGIN      
        1 ttttagaaat taaattatat gtgaaaaaag atagcttaca ttattttatt atggaaaata
       61 atagtaaatg gtacaaaatt tgatttttaa gatttaaaac ttttttaaaa agtgctacaa
      121 tagaccaaat tattactttc tatactcgtt aaacaggagg tgggactatg aaaaaacata
      181 atattttatt tgtatcaact gatgacaaaa taaatataga tatatcaaaa caattggaaa
      241 atatatttgg agaattttgt aatatagata acttggtata tgtaaatagg ataaatattg
      301 aactgtcaag ctatgagctt gtagtatgtt cagacaatga tataaaggaa tatatacata
      361 ataatattga taaaaatata cctattgtaa tagtacatcg tactataaat attgaaaata
      421 taaatcagat aataagtata gaaaatgatt ctgatgttat ggttatagat gcttataaag
      481 agtcagcaga tgaaactgcc aagataatta gaaaactagg attaattcat attaatctaa
      541 taccatatta tccaggttgt gataagtcta aatgtgagat tggaattatt acgggaagca
      601 gaaacagcat accacaaaac ataaagcaaa taattgacat aggtgataag gtaattgata
      661 taaatacagt tatagaaatc tttacaaaac taaatatatc tatagataaa ctacatatta
      721 ttaaggaaaa atacaatgaa gatacagtaa gtggatatag gtattataca actatgaata
      781 aaaccatgaa gagttttttg gagattatag atgaaggtat tgcttctgtt gataaattag
      841 gaaaatttat atattgcaac aaagtatttt ctaatctagt tggaatagac caaaatgaaa
      901 ttatatctaa taactttatg gacttattta gtgataaggt tgtcaaaaaa atattttttc
      961 aagaagatga agttaatgat gaagtagtaa atttaaataa caaaaaactg ataattaata
     1021 aagtaaatgt ttatgaaaat aatgaacgaa taaagagtat tataagcatt aaaaatataa
     1081 gtgcaattca agtgcttgag gacaagattc aaaataaact tcagtcaaaa ggttttgtat
     1141 caaagtatac atttgagagt gttgtaggtg agagtaaaat aataaaagaa aaaattaata
     1201 ttgcaagaaa aatagcaact acagactttt ctgtgttgat acttggagaa aatggaacag
     1261 gaaaagaaat atttgcccag gcaatacata atgaatctct aagaaaaaac aagccatttg
     1321 ttgcagttaa tctatcttct ttgtcagata cacttattga aagtgaactt tttggctatg
     1381 atgaaggaag ttttacagga gcaataaaag gtggaaaaat gggtatattt gaaagagcac
     1441 atacaggaac aattttttta gatgagatag gtgatatatc tttagatgta cagcagagat
     1501 tgctaagagt attacaggaa aaagaagtta tgagaatggg cggaagtaaa attataccaa
     1561 tagatgttag aattatagct gcaacaaata aagatttaaa gaaaaaaatt aaagaaggtt
     1621 cttttagaga agatttgtat tataggatta atgtattaca tatagagata cctagactta
     1681 gagaacgtaa ggaagatata cctcttataa gtaagtattt tttagatgaa ataaatagca
     1741 acaaatgttt tacagaggaa tcaatgcaag ctttaacatt gtatgaatgg ccaggaaatg
     1801 taagagagct taaaaattta atttattata ttgatactat agttgaggaa gatagagtag
     1861 attacgagca tttgccagaa caatttagat ttgaaaaaaa caatacttta gttaatgaaa
     1921 atttcgattc aattatctta gatttcaaac aatctaattt ctttgaggaa agtatatgta
     1981 tattgaccag tatagaaact tggaataata aaaatatatt attaggaaga aataaactgc
     2041 aagagatttt aaaagaaaaa gatatagttt taagtgtaga ccaaattaga aaaagaatag
     2101 ataagttaaa gagccatggt ttacttttat ctggagtaaa aaaacaaggt agttttataa
     2161 ctgatgaagg aaagaacttt atatcatatg taaaaattaa gggagtgata taggggacta
     2221 aaaaggcaaa acaccctaaa aaaatcccta taatggatag aaataaataa aattatcttt
     2281 aagattaaga taattcaaaa atattgaata aataatctaa gaagattatt tttaataaaa
     2341 cagtagtaaa tatcaacctt actactgttt ttattttttg caagatttat tatacaatta
     2401 aaatttatat tttggcacat caattgctac ataggaaaaa tataaaaaat tgtagaactt
     2461 ggaggatgtt gttatgaagt cagcttacaa aggatatgaa gcctatagtt atttagaaaa
     2521 aggaaaagat atttcatcta ttgagatgtg taaaggagat aaaagagtta aagaatacct
     2581 tattgaatta gatgatattc aagaagctaa ggttaaaagt atagtggatg aaaaaatatt
     2641 tatatcatta catgaacatc cagtattatt tccagaacac ctagaaagag atatatttga
     2701 atataatagg gaaggtaaac aaagatgtgc ttatgaagca ttatcaagag gatattatga
     2761 tgctatattt gataatttaa tggatggtgt atgtacgata acatcaaatt cagggtggaa
     2821 atgggatgat atattaatag atttaggcat gagactatgt gatttagctc atcaagattt
     2881 tgttataaag tgtgaaaaag tagaagatat atacagagct aaaagtgaag gtaaagtagc
     2941 attgatacca acattagaag gtgcagcacc aatagaaaat gaacttgata gaatagatat
     3001 actgtatgga tttggtataa ggttaatggg tataacatac agcgaatcca atgcattagg
     3061 ttcaggacta aaagaagaga aagatggagg tcttacaaat ttcggaaaaa aagctgttga
     3121 aagaatgaat aaaattggaa tggcaataga ttgctctcac gttggagttc aaactacact
     3181 tgatgtaata gaatatagca aggacccaat aatattaagc catgttgggg caagaagttt
     3241 atggaacaca aagagactaa ctccagatga agtattaata gcatgtgcta aaaaaggagg
     3301 ggttataggt gtagaagcag cacctcatac tacaataaca gagaagaata aagaacattc
     3361 aatagagtca tttatggaac attttgagta tattaaaaat ttagtaggaa tagaccatgt
     3421 agcttttgga ccagatacag tatatggcga ccatgtaaaa ttacactcag tactagctgc
     3481 taaatttgat ataaaaaaaa taacaggaac aaaagaacat cccaaagtag aatatgtaaa
     3541 aggtcttgag aacccaacag aaacttcaaa aaacattgtt agatatttag taaagcatgg
     3601 atatagtgat gaggaaatag agaaggtact aggtggaaat atacttagag tactaaaaca
     3661 ggtttggaaa tagatatatt agaaatatta ataaaatttg aggatttata gggaggtttt
     3721 ataaatggat tatttaaaat tagctaatca tccacttttg tggatagcga gtacagtagc
     3781 agtatcaata gtagtggtac aatcccttat atttgctata aaatcttgca aagttggtaa
     3841 aacaatggga ataacagata aacaaataaa gtcagctgtt aagagtagtg ctatttcagc
     3901 tattgggcct tcaatgacta tatttgctgg aatggtatcg cttatagtaa ctatgggtgg
     3961 gcctatagca tggatgagat tatcatttat aggttctgta atttttgaat caatgtcagc
     4021 cggatttgga acagatgcat taggtataac tttaggttct cctgaaatga caaaacttgc
     4081 atttacaaat gcagtttgga ctatgatatt aggttcttta ggatggataa tattcacatt
     4141 actatttgga cataaactag ataagataac caatgttata tctagtggca agaaatcatt
     4201 tataccaata atatctctag gagcaatgtt gggttcattt gcatatctaa atgctgatag
     4261 ggtgttgcgt tttgataatg gaacaattgc atgtataagt ggaatggcta taatggtaat
     4321 attgtgtatg cttgaaaaga agaaaaatat aaaatggtta agagagtggg ggcttacaat
     4381 atcaatgttt agcggaatga taatagcttc agtaatataa ggggggagtt ttatgagtaa
     4441 agtaaaatct atatacaatg aagaatattt accttttatg ataaggtatg gaagactaac
     4501 actatctttg ggtattatag cagcattagt tcctggaata attttgagct ttggatttgg
     4561 tattatgcct ccaatatcag cattattagc aagtacaatg gcaattgtca gtatgagtgc
     4621 tccaaattac ataatagaac cagtttctta ttcaccaatc ttaggtatcc caggaactta
     4681 tatgagtttt ctttctggta atatatctaa tatgaggcta ccatgttcaa tagcagctca
     4741 aaaggcagca gaagttgaat caggtacaga agaaggctcg ataatatcta caataggaat
     4801 agctgtatct attttagtga atataagtat actaactata ggggtaattt taggaggaag
     4861 cgtactatct aaaattccag ctgaagtagt agaaaaatta aacttaattt taccagctct
     4921 atttgggtca gtttttggac aagtattttt acaagataaa aaattaggtc tagtagcaat
     4981 tgtaatatct gtattgacta ttatattatc aaagcaaggt ataataccac agtctctagt
     5041 agttcttata tgtgtatttg gtactatatt aattgcaaga gctatgtata aagataagct
     5101 tagtgactaa ttacaatgaa ataatagtga attttcaaat atgaaattta ataatttgat
     5161 tttcaaatat aaaaattaca ttaagattat ttaaaaattg gtttttaagg agggaatatt
     5221 ttgatatatg atttaatctt aaaaaatgga tttataatag atggtacagg taatcctggt
     5281 ttttatggag atatagcaat taaagacaac ttaatagcaa aaatagattc taagataaat
     5341 agcaatacaa ataaagagat tgattgttat ggaaaaatta taacacctgg atttattgac
     5401 cctcatgttc atgaagaaat agtagcaata ctagatggta aatttgaaaa gtttctaaaa
     5461 caaggagtta caacaactat aaatggtaat tgtggacatt ctataacacc atattcttct
     5521 gaaaatgtat atgaatatat gtataaaaat ggtctattac ttgaagaaga aaaaaagtat
     5581 cttatagata aaaataaatg ttggaataat tttacagaat attgtgacct tatatctaaa
     5641 agtggtattt ctgtcaatat gggattcttg ttggggcatg ggactataag atggagtgtt
     5701 atgggaggtt ctaaggatag acctcctaca gaaaaggaaa aaaatgaaat tacagatata
     5761 ataaatgatg gaatgaaaag tggtgctttt ggaatatcta ctggattggc atatatacca
     5821 agtaagtatg ctgatattga tgaacttgtt gatattgcaa ggcagattaa agagcatgat
     5881 ggtatatata catctcatat tagagattac ataggaagat ataatgcagt aaaagaagcc
     5941 atagaggttg ggcagaaaag tggtgctagg gttcaagtat ctcacttaag tccagttgaa
     6001 atagaagcat ttgatgagat attaaaggct aggtataatg gtgttgaaat aatggttgac
     6061 acagtaccaa gaagttctgg gcattgtatg aagaaaaaaa gagtaattca atttattatg
     6121 gcaatatcat ctagtctatt tgaattaggt atagatgggg taatggatgc tcttagaaat
     6181 gaagagggaa gaacacttat attaaaagaa gcatttatac ttggagatag ggggagtata
     6241 atacttctaa acactaaaga tattaatatg gaaaagaaat caatacgtga aatagctaca
     6301 caaaaaggaa tagatgagga caagttatta ctcgatttac ttttagatgg agacgaagaa
     6361 cttatctttt gtttaggagg aatgtataga gctgatttcc cagataaact acatgatgat
     6421 aaaataattg ataatccatt tgttatggtt ggttcagatt gtttgtttag tgtgggtggg
     6481 gatatgtcat ggtttgaact acaaagaaga ggagcttttc caattttctt caatatgtac
     6541 agaaagtctg gtgttagatt ggaagagatt gtaagaagag ttacttcatt accagctagg
     6601 caatttaaaa taaaaaatag aggtattctt aaagaagggt tgatagctga tatagcagtt
     6661 attgacatgg ataactatag ttatcctagg tcagaagata ttgatttttc aaagccacaa
     6721 ttactagcca atggtgttga atatgtaatt gtaaatggaa aaattgcgct agaaaatgga
     6781 gagattactg agaataaatg tggtgaagta ttaaaaagat aaggttttaa aaataatagg
     6841 atttagaaag taaataaata tctaagtaag taaagacgaa
//
LOCUS       DAYASV010000175         6390 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYASV010000175 DAYASV010000000
VERSION     DAYASV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.180"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            283..1227
                     /locus_tag="ACGI68_003454"
     CDS             283..1227
                     /locus_tag="ACGI68_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFH0139857.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1249..1875
                     /gene="hisG"
                     /locus_tag="ACGI68_003455"
     CDS             1249..1875
                     /gene="hisG"
                     /locus_tag="ACGI68_003455"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFH0139858.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1909..2958
                     /gene="hisC"
                     /locus_tag="ACGI68_003456"
     CDS             1909..2958
                     /gene="hisC"
                     /locus_tag="ACGI68_003456"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0139859.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2961..3539
                     /gene="hisB"
                     /locus_tag="ACGI68_003457"
     CDS             2961..3539
                     /gene="hisB"
                     /locus_tag="ACGI68_003457"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFH0139860.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3541..4146
                     /gene="hisH"
                     /locus_tag="ACGI68_003458"
     CDS             3541..4146
                     /gene="hisH"
                     /locus_tag="ACGI68_003458"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFH0139861.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4143..4865
                     /gene="hisA"
                     /locus_tag="ACGI68_003459"
     CDS             4143..4865
                     /gene="hisA"
                     /locus_tag="ACGI68_003459"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFH0139862.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4847..5605
                     /gene="hisF"
                     /locus_tag="ACGI68_003460"
     CDS             4847..5605
                     /gene="hisF"
                     /locus_tag="ACGI68_003460"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFH0139863.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5633..6301
                     /gene="hisIE"
                     /locus_tag="ACGI68_003461"
     CDS             5633..6301
                     /gene="hisIE"
                     /locus_tag="ACGI68_003461"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFH0139864.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDVIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2610 a          537 c         1260 g         1983 t
ORIGIN      
        1 aatattaata aaaattattt gggaggaaat tatcatggtg agattaaata tagtaaaatt
       61 aaatacaaaa ttaaatcatc accatcatca tagattaaag ggattgtaat atgaaattaa
      121 ttcatagaca caagccctgt ttttagtgta attggtcttg tatctatgat gggaagtaat
      181 tttaagagaa aattatagcc atataggtat aaagcctatg tggctttttt attgttttaa
      241 atttgcaaat ataagagtaa atttaatgaa gagggtgaga atgtgaaatt tttgaggatt
      301 gaagatgagg ttatttattc taaaaaaaga tataaattaa aaagggaaat agataaactt
      361 tttttggatg aagaatattt tcaaatagaa ccacaattat ttgaagaata tgatgaattt
      421 acaactataa ataataaaat tccagaagaa tctatggtaa aggttgtaaa tggaaaagta
      481 atggtactta gagctgatat aactacaagt ataataaaaa gccttgtacc aagatgggaa
      541 gatggtttaa agcttaagct tttttataat tcatcaatat ataaaaataa aaatacagtt
      601 ggaataaaag agataagaca aataggttgt gagtatctag gagaagcctc tgtagaagca
      661 gatagagaag tagtgaaatt agctcttaag atacttgaaa aatacaataa taattttatt
      721 ctagaggttg ggagtagcaa gtatattcat ggtttgttag aagaattaaa tttaaataag
      781 aattgtgaaa atcaaattaa aaatttacta tatacgaaga acactcatga actaaaagta
      841 tatattgaag aattaaaaat taagcgtgaa gtaaaagagt tattatcaaa tattttaagt
      901 ttacaaggga atttatataa tgttattgaa aaatcaaaca agttctattg taataataag
      961 atgaagcaag ctcttgaaga attaaagcaa gtaaataatt taattgagga atgtaatttt
     1021 ttagataagg ctagatttga tttatcaatg attactatgc ttgactatta tgaaggaatt
     1081 atgtttagag gatactaccc aaactcatat aaagaaattc ttagtggcgg aagatatgat
     1141 tctttaacta aagagtatgg aaaagaaatt ccagccattg gatttacact aagtgttgat
     1201 gagttaatga agtatgtata taagtaaagt ttagaaggag ggattgcttt ggattatttg
     1261 actatagcac tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt taaaaaaatg
     1321 ggtctaggag acagtataga cacagatacg agaaaactaa tttttaaaga tgaagaaaat
     1381 aaaattatat atatacatgt aaagccatca gatgtagtta catatgttga aaaaggagtc
     1441 gctgacctcg gtatagcagg taaagatact attttagaaa atgagacaga tgtatatgaa
     1501 atatatgatt taggttttgg aaaatgtaaa tttgctgtag caggattaaa gggagatagc
     1561 atatatagag aagatgaata tttaaaagtg gcaacaaagt atcccaatat agcaaaaaag
     1621 tattttaaag agaaaggtca aaaaattgaa ataataaaat taaatggttc tgtagaacta
     1681 gctccaatag tagggttgtc agatgtaatt gtagatatag ttgagactgg caatacactt
     1741 aaagcaaatg gtcttgaaat attagaagac atatgtaata taagtgctag aattatatca
     1801 aatagagcta gctatagatt taaatatgaa caaattcaga atataattcg tttatttgaa
     1861 gaacttgata attaaaatat aattgagctt tataaggagg gatttaagtt gagagaaaaa
     1921 gaaagcataa gagagttaag gggatatgaa ccaaatcacg taaactgtaa agtaaaactt
     1981 gatgcaaatg aaggaagtaa gaggttgttt aaatacctta ttaaagaaat ttcagatagt
     2041 gatatagatt taaatttata tcctgaagat tcttatagtg atttgaaaga atctataata
     2101 gattacataa atatttctgg agtaaataag aaaaatcttc tagtaggaaa cggttcaagt
     2161 gaaataattg atttaataat acatactttt gtagataaag atgaagtaat attgagtttc
     2221 tctccaagtt ttagtatgta ttctatatat agtcagataa atgggtctaa atttataggt
     2281 gttgaaagtg atgaaaattt agttataaat atagataatg tgattgaaaa agtaaaggag
     2341 aataatccta aaatagtaat tgtatgtaat cctaataatc caactggaac aatattaaag
     2401 agggaagaga taataaagct tttagattca actaatagtc tagtggtttt agatgaagca
     2461 tatatggatt ttggagaaga aagcatgtta agtgatgttt ttaagtatga taatctaata
     2521 gttcttagaa ctttatcaaa ggcatttgga ttggctggaa taagaacagg atatatgtta
     2581 tctaatagta gtttaataaa ttctgttgag aaagtaagac ctccatataa tttaaattca
     2641 ttatcagact ttattgctac aagagcactt agaaataaag atgtggtaaa ggcttatata
     2701 aaggaagtaa aagaggaaag agaagtttta tatgaagaaa tgattggtat gggaattaaa
     2761 gcatacaaat cacaagcaaa ttttatatta ttttattctg aaatcgaaaa tttatcacaa
     2821 aaacttattg atagaggtgt tttaataaga aaatttggtg gaaagttaga aaattattac
     2881 agagttacaa ttggtgataa agaagaaaat tctatgtttg ttggagctat aagagatatt
     2941 ttgaaaaagg agaaataggt atgagaattt ggaaggtaga aagaaatact cttgaaacac
     3001 agattttagt agaattaaat attgatggtt ctggtaaagc tgaaatagat actggaatag
     3061 gatttttaga ccatatgctt accttaatgt catttcatgg caagttcgat ttaaaagtga
     3121 tttgtaaggg tgatacatat gtagatgacc accatagtgt agaagatata ggtatagcta
     3181 taggtgaagc atttaaaaat gcactaggtg acaaaaaagg aatacgaaga tattctaata
     3241 tctatatacc tatggatgaa tctctatcaa tggtagcaat agatataagc aatagacctt
     3301 atcttgtatt taatgctaaa tttgataccc aaatgattgg tagtatgagt actcaatgct
     3361 ttaaagaatt ttttagagct tttgtaaatg aatcgagagt aacattacat atcaatctct
     3421 tatatgggga aaatgaccat cataagatag aaagtatttt taaagcattt gcaagagctt
     3481 taaaagaggg tagtgaaata gtttctaatg agatagcatc ttcaaagggg gttttgtaga
     3541 atgaatataa tagtagacta tggtcttgga aatatagatt ctgtgtctag gggttttaga
     3601 aaagctggaa tagaaactaa aatttctagt gatatagatg aaataaaaca ggcagattca
     3661 cttatacttc caggtgttgg agcttttaga gattctataa gtgccttaga taaactagga
     3721 ttgataccaa taataaagga gcatgtttct aagggtaaat ttatgatagg tatatgtcta
     3781 ggaatgcaat tactttatga aaaaagttat gagtatggag aatatgaagg attaggactt
     3841 ataaaaggaa gtattgataa actggatata agtttaaaag tgccacatat gggatggaat
     3901 aatctaaaat ttaataaggc aaatgatgat atacttaaat atataaatga agatgattat
     3961 gtatacttcg ttcactcata ttatgcaaat tcatcaaatg aagaactaat tgcattttca
     4021 gaatatgaaa agaaaatccc tgctattgtt agaaaaggca atgtatatgg tatacaattt
     4081 catcctgaaa aaagtggaga agtagggtta aatatattaa gagcatatgg ggagatgata
     4141 aaatgataat atttccagca atagatataa aagataataa gtgtgtaaga ctcacacaag
     4201 gagaatttga caaagtaaat gtatattatg ataatccatt agaagtagca tataaatgga
     4261 aaaatgaagg agcagaatat atacatatag tagatttaaa tggtgctaga agtgaatttg
     4321 gtgtaaatac aaaaataatt gaggacatag caaacaatat agatatacct attcaagttg
     4381 gcggtggagt tagggataag gaaaaagtca aaagtttaat aaatgctggt gttactagag
     4441 taatattagg tagtatagcg attgaaaatt tgaatttagt tgaagaattg gtcaatgaat
     4501 ataaagagaa aattgtagtt tctatagatg ctaaagatgg aaaagtagca gttaggggat
     4561 gggaagttgt aagcaatgta gactcattaa cattatgtaa acaacttgaa aagataggtg
     4621 tacaaactat tgtttataca gatatatcaa aagatgggat gttgcaaggt ccaaattttg
     4681 acatatatga aagaatagca aaggaaacat cactaaatgt tattgcttct ggaggcgtaa
     4741 cttctataga agacgtaaaa agactaaaag ctatgaactt atatggtgca ataataggta
     4801 aagcgcttta tgataaaaag atagacttta aggaggcaca acaattatgc ttactaggag
     4861 aataatacct tgtctagatg tgagaaatgg aagagttgta aaggggaaaa aatttaaaga
     4921 tatagtagat gttgatagtc cagaagtact tggaaaattt tatagtgatt gtggtgcaga
     4981 tgaacttgta ttctatgata taactgcatc aaatgaagag agaaagacat ctttagaatt
     5041 tgtgacaaaa gttgcagaaa atataaatat accattttgt gttggtggag gagtaaataa
     5101 attagaagat ttcactgata ttttaagaaa aggtgctgat aaagtttcta taaattcatc
     5161 agcagtcaaa aatcctgaat taataaggga agcttcatta aaatttggag cacaatgtgt
     5221 tgttttatcg atagatgcaa agaaaaatga agagggttca tggagcgtat acgtaaaagg
     5281 tggaagagaa aaaactaatc tggatgcaat tgaatgggct gttaaaggtg tagaacttgg
     5341 agcaggagaa atcgttgtaa atagtatgga tgaagatgga atgaaaaatg gatatgatat
     5401 agaattatta tcaaagataa cttctttagt aaatgtacct gttatagcat ctggaggcgc
     5461 tggaaaaaag gaagattttt atgaggcggt taataaatcc aatgtagatg gaattttagc
     5521 agcatctgtt tttcattttg gcgagataaa aataaacgat ttaaagaaat atttaaaaga
     5581 catgggtgta gaagtaagat tataagttaa atactaaaag gagattagaa atatggataa
     5641 taagtgtaac aatgtataca gtgatgaagt agagaaattt ataagaagta taaagtttga
     5701 tgataaaggg ttagtccctg ttgttgttca agaggtagtt agcaaagatg tactgatgtt
     5761 agcatatatg aataaagatg tgataaagaa aaccttaaaa gataaagtag cttgctattt
     5821 tagtagaagt agacaagaac tttgggttaa aggtgagaca tctggtaata ctcaaaaagt
     5881 agttaagatg tcttatgatt gtgatgtaga tactatacta ctttttgttg aacaaactgg
     5941 tgtagcttgt catacaggca attattcttg tttttacaga gacttgtttg atgatagttc
     6001 aaaaatggag cttgaagtac aaacaaatat attgaaagaa ttatatgatt taatcaatga
     6061 aagaaaaaat aatccagttg aaggttctta tactaattat ctttttgaaa aaggaataga
     6121 taagattctt aaaaaagtag gtgaagagag tagtgaggta attatagcat caaaaaatac
     6181 tgataaaagt gaacttattt acgaaattag cgatttagta tatcatacat tggttttaat
     6241 gatagaaaaa ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa gaagaaaata
     6301 gaataagata gattaatggg taataagaaa atataccact tttaataaaa agtggtatat
     6361 tttttatttt atattttgat ttatagaata
//
LOCUS       DAYASV010000176         6285 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYASV010000176 DAYASV010000000
VERSION     DAYASV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.181"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            294..1661
                     /locus_tag="ACGI68_003462"
     CDS             294..1661
                     /locus_tag="ACGI68_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFH0139865.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            1686..2294
                     /locus_tag="ACGI68_003463"
     CDS             1686..2294
                     /locus_tag="ACGI68_003463"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFH0139866.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMDDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            2342..2713
                     /locus_tag="ACGI68_003464"
     CDS             2342..2713
                     /locus_tag="ACGI68_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFH0139867.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            2892..3230
                     /locus_tag="ACGI68_003465"
     CDS             2892..3230
                     /locus_tag="ACGI68_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFH0139868.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            3696..4199
                     /locus_tag="ACGI68_003466"
     CDS             3696..4199
                     /locus_tag="ACGI68_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HFH0139869.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGVYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            4246..5205
                     /gene="moaA"
                     /locus_tag="ACGI68_003467"
     CDS             4246..5205
                     /gene="moaA"
                     /locus_tag="ACGI68_003467"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFH0139870.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            5209..5679
                     /gene="moaC"
                     /locus_tag="ACGI68_003468"
     CDS             5209..5679
                     /gene="moaC"
                     /locus_tag="ACGI68_003468"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFH0139871.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            5715..6146
                     /locus_tag="ACGI68_003469"
     CDS             5715..6146
                     /locus_tag="ACGI68_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HFH0139872.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         2662 a          504 c         1131 g         1988 t
ORIGIN      
        1 aaaaaataat ttttatatag taaatgacaa aagtttacaa taaatgtata ttttcttact
       61 cattccttaa aattactcat ttagtttaat aactctcatt tcatatgtaa agttggtaaa
      121 aaaattaaaa ttaaaagtaa aaaactaaat aaattaatgt taatttgaaa tatatatgaa
      181 ggtttttgga gaatacttac ttatgatagg aaaaaatgtt aaaataatag tgtaatcaaa
      241 tataaaggac tgaaataatt aacagttatt aaatattagg agtgtaaaaa tttatgggat
      301 taaaagaatt tgattttatt gaagagtcta ttgatatgtt gaggacaatg tcaccaacat
      361 tagaaactat atcagatgaa atagaagaat attttgaaaa tatcttggat gaaaagaatc
      421 aagaatatat aaatgtaacc tctagaataa aatcagaatc aagtttaaga gaaaagataa
      481 ttagaaatag atatttaaaa aaatatggag aagcaagtaa tcttattcat aatgtttctg
      541 acttaatagg cctaagaatt gagtgtagat ttattgagga tgaaaacaaa atatatagac
      601 ttttgagaag atattttaat aaaacagatg acaaaataaa ctattataat aaagagaata
      661 agaatattaa attaaaacta tctgagaggc aacccaataa gcaaaaaaat ggatttgaaa
      721 tatataaaat agatggagtt tttgcgtatt tagatagaga agtaaaattt gaacttcaaa
      781 ttaaatctct tgtaaatgtg ttttggagtg aaatagaaca taaaataatt tataaaaaca
      841 atacatattt acttgctgat aaatttataa aggatatgat ggactctata aagaataatc
      901 ttactatgat agataatcag cttttaagta tttataaaaa ctttcattca gggaagtctt
      961 ttaatatgaa agtaagtaaa aaagaaattg aaaaattatt tgcgaaacta gtctatgacg
     1021 ctttttctga aaagatgaat aaaagtatag gttttgttgt agattttaaa aaaccatgtg
     1081 agactatatt gaactattct tttaataaac aagatataag tgatgaagtt ttaggtaatt
     1141 ttatgcttga tgagtttgca agattaaatg aaattgtaaa caaggatata gattttaatg
     1201 agcaaataga atttgaaaga gaaccaacat ttgatgataa gttttgtaaa gacctaggaa
     1261 atcattttag aagtagatta aacacagaat ttccatggaa cctatttttt agaatattat
     1321 ttgaaataga gccatataac aataccaaag actttgaaaa ctttgtagag tttataaaag
     1381 aaaatgtgct actagaagaa aatagggagc aattattatg ccaatttgaa gaggatagta
     1441 agcttataat tgaagatata tatgattgta tatttaatag tataagtgag attgatagtg
     1501 ttgaaatttt atataactat aatcttgaaa aaataaactt tacatcaagt gaaatcataa
     1561 attgtatatg tagagaatat gagtgttatg gtgaatactt agaagaaaaa gaaaagctta
     1621 tgaatacatt taaagaaaag attattcaaa tctttgagta ataggtcaat gctgaagtgg
     1681 cgctaatgcg ccatttttca aatatatatg aattaagtaa aattattttg gaggaggcga
     1741 tttgtataga gaaaacaggt gtaattttgg cagggggaaa aaattctaga atgggtagag
     1801 ataaagcctt tttagagtta catgataagc tttttattga aattgcaata gaagcattta
     1861 aaaactttga tgagttgata ataatatcta acaatgaaga attatattct aaatacgata
     1921 ttaaagttta taatgatata gtaaaagatg ttggtccaat tggaggaata tatactgctc
     1981 ttgaatatgc aaagtatgat atagttacaa ttgcatgtga tatgccatat ctaaataatc
     2041 aaatagtcga aagaatagct aataaaatgg atgataaaag tgtgatttca gtgacaaatg
     2101 gaaagttaca accattgtgt tctggatata aaaagagtat aataaataaa gtatctttat
     2161 gtataaggga aaatgattta aagctcagaa gctttataga taaaatagat aaaagttata
     2221 tatactttga tgaagaagat ttatttttaa atgtaaacac tgttgatgag tataaaaaat
     2281 taactaaagt ttaattagct atggttgtaa aatagagtag gatttatatg gaggagagtt
     2341 tatgaattta cttaaatgcg attcttgggc agtaatatta aataatagcg ataaagaaag
     2401 taaagcatat aaaatattag atgaacttaa aaggaatatg tacaaagttg ttgctataga
     2461 tgaagagaaa aaacctattg aaggaataga tgtgtatgaa tgtttaaaag atgttccaca
     2521 caatattgat gttgtagcta ttattgataa acaatctaag atggatgtga ttttagaaga
     2581 agtagagctt ttggatatac aaaatatgtg gtttgaaaaa ggaagcttta gtgaaatgat
     2641 gattaaaaag actaaagatt taaaattaaa tatagaatat aatttaagtt tatatgatga
     2701 gttgactaga taaaattata tttcttaata ataaaaatat aattagttaa taaaacatat
     2761 tattttatag tatagattgg ttaataaaag tacagattgg ttaatgaaac attaaattac
     2821 aatatttgtt aaaaaaattg tgagttacat gatataatgt attaaatagg atactaagga
     2881 gttgatatat tatgttaata gtaactacag aaaaagtaga aggtaaaaag atatcaaagg
     2941 ttttaggatt agtgagagga agtacaataa gagcaaaaca tgttggaaaa gatataggag
     3001 caagttttaa gaatcttgtt ggaggagaac ttactggata taatgaaatg ctcactgaag
     3061 caagacaaat tgccataggt agaatggttg aagatgcaga agctaaaggt gcaaacgcag
     3121 taatagcatt tagactgtct tcagcttcag ttatgcaagg agcagcagaa atgcttgctt
     3181 atggaacagc agttgtttta gaagatgata atagtattct tgaaaaataa aatataaata
     3241 gttaaaaaga ggcatctttt aaaaatttta tggatgcctt tttttatttg aaaaaactaa
     3301 tttgagaaga aattttaact attatgacaa taaaattagg gtgatattat ataaataagt
     3361 aaaattatat tatataatgt atggtataga ctttaaaaat tttatttgta gagaaaaata
     3421 tggacaatgt tataataatt atagaaatta aaagtactta cattcattat gtatgtgaca
     3481 tgtataatta gttttatatt taatacagtt ttgaagtaat atcttcattt tgagaacgtt
     3541 ggaataataa aaaatgtatt attataggtg atataaataa aaaataaata taatatgtta
     3601 tattgctgat agaaaagaga ataaaagaaa ataggtttta ttaaaagaga tttttattta
     3661 taggacttaa tcaatgtata gaggggtgag aacatatgtt taatgttgca attataacac
     3721 ttagtgataa aggatatgaa ggaaaaagag aagatataac aggaaaaaag ttaactgaat
     3781 ttgttgaaaa tacaggagtt tataaagtta ctgaatatgt acttataaaa gatgataagg
     3841 aaatgctaaa ggaaaatatt ataagacttt gtaatagtaa taaaatagat ttaattctta
     3901 caaatggagg tactggtttt tcaaaaagag atataacccc agaagcaact aaggaagtgt
     3961 tggaaaaaga aataccaggt ctgagcgaat atatgagaat gaaatccaca gaaattacta
     4021 aaaaggccat actaagtagg ggtgtgagtg gaataagaaa taattctata ataataaatt
     4081 tacctggaag tcctaaaggt gcagttgaaa atctaagttt tataattgat gttttagacc
     4141 atggcataga agttttaaga ggagaagcta cagaatgtgc cactaaaaaa agtgaatagt
     4201 atataatcta gatagataaa aaatagacaa agaggtagat aaagtatgat tgataagtat
     4261 ggaagaaaaa tagactacct tcgtatttca cttacagata agtgtaattt aaggtgtgtc
     4321 tattgtatgc caccagatgt aaaatttgat aaaaattata taaatgaaaa tctaagcttt
     4381 gaagactaca agtttattat aaaggctatg gctgaacaag gtataacaaa aattagattt
     4441 actggaggag aacctctttt atatcctaaa ttaaatgagc ttataaaatt tacaaaagaa
     4501 gaatgtggaa taaataacat aggaatgacc actaatgcta ttggtctttc tgatagaatt
     4561 tttgagcttg aaaaatgtgg cttaaataaa gttaatataa gtttagattc tttgaaggaa
     4621 tataagtata aatccataac aagaggagga aacttaaaag atgtcttaaa atcgattgaa
     4681 tcttgtttaa atttaggtat aaaagtaaag ttaaattgcg ttgctataag tgggtttaat
     4741 gatgatgagc ttaaagattt tatgttttta acagttaaat caccaattga tgtaagattt
     4801 atagagctta tgccattagg tgaggctaat agagtttata agaagggtta ttttaatgta
     4861 aaagaaatga ttgaaaaaat atatggttta tataaagtca aaaatgatga aaaaagtaca
     4921 gcagaatatt atatgtttaa aggagctaaa ggaagaatag gaattataac tccattaagc
     4981 tgttcatttt gtaacacttg taatcgtatt agacttactt ctagtggtgc aataaaatta
     5041 tgtctacatt caaaagaaga aatagatata aaaccatttt tacataaacc attgttattt
     5101 agagaatcta tgaaagatat aataaaacaa aaaccagaaa aacatcattt aatagaaaat
     5161 aaatgtagtg atacaaatag gcgtatgtat gaaattggag gataagtaat gttaacacat
     5221 ataaatgaaa aagggtatgc caagatggtg gacataagtg aaaaagatga tactaaaaga
     5281 acggccgtag ctacagctac aattactctt agtaaggaag cattagaaaa agtaaaaaat
     5341 ggtcagataa aaaaaggaga cgtattgtct gtagctcaag ttgcaggtat aatgggagct
     5401 aaaaatacat cttcaaacat accaatgtgc catcagatta aattagttgg atgtgattta
     5461 gaatttgatg tagatgattc tatgtctgct attaagatat acgcaacctg taaatctctt
     5521 gggaaaactg gagtagaaat ggaagcttta aatgcatgtt ctttagcagc acttacaatt
     5581 tatgatatgt gtaaagcagt tgataaaaaa atggttataa gtaatatcca tcttgtggag
     5641 aagactggtg gaaaatcagg agattttaag ttcgagtaaa ttaataatat attgggttaa
     5701 aatttggagg aaatatggcg aaggtagtgt caataaatat aagtgagaaa aagggtgtaa
     5761 ttaaagttcc tgtacaatgt gcagagctaa aaataaatca tgggatagtt ggagatgcac
     5821 atgcaggaaa ctggcatcgt cagataagct tacttggaaa agaaagcata gataaaatga
     5881 aaatgaaagg ctttgaccaa ttaaaatatg gagattttgc tgaaaatgta actacagaag
     5941 ggatagaagt tttttccctg cctgttggta caaagttaag aatagggcaa tgtgaagttg
     6001 aagtaactca aatagggaaa aaatgtcaca atggatgtga aattaagaaa ttgacaggtg
     6061 actgtgtgat gccacgagaa ggaatttttg taaaggttct aaaagaagga attataaatg
     6121 tgaatgattc aatagatgta atctaatgaa gtcactatta aaatataatt ataagtaaat
     6181 actattaaga tttaaatata tattttaaga gagaattaat tttaataaaa tattttagtt
     6241 ataaattgga atattggtta tataaagcta tgaatgtaaa atcat
//
LOCUS       DAYASV010000177         6257 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYASV010000177 DAYASV010000000
VERSION     DAYASV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.182"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(145..567)
                     /locus_tag="ACGI68_003470"
     CDS             complement(145..567)
                     /locus_tag="ACGI68_003470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139873.1"
                     /translation="MDNKVNEVITKYEIKKKQFLNTRVDSFTIKDIEILLNEIEILNS
                     EITKTNDTIDTLNKDIKYLKYLNKKAKNIIKIYREELEEFKTKAFIPKRKKQQITQKD
                     IDKIKKLSQDGLSYSKIQKITKWSKCTISKVINGYYDK"
     gene            complement(668..2521)
                     /locus_tag="ACGI68_003471"
     CDS             complement(668..2521)
                     /locus_tag="ACGI68_003471"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFH0139874.1"
                     /translation="MEKLNLEWVTDIIGEDYKKWNMEDRHIINNRNNVFIETQTGTGK
                     TKFIIDKIVPLVNQSIRNKRKLNLLIICNRINLKRQVKLELLKSNLDLYEFDKDIKKI
                     CNSDNTENNIKETFQILDKLSKITNGITIMSYQQLGEIIYYNNFYCNQHKNNLNFFDF
                     IVFDECHFMLSDLEFNTKCNLIFNELVLKEYSNSFRIFLSATLYELKNAIDFNARLEN
                     SRGEARKNNCNFNIYTFFNQYISLNHTNNFKEKTNLNNEYEFSTGRDYSYLDVKYFKN
                     IEDIIILLKNHFSANKDKWLIFVSKIKDAQYIKERLKSEKISIEIFKSGSNTKEAENI
                     VHNSSFECDVLISTKVLDNGINLKDKSIRNLVVMELDKTTFIQEIGRIRVDIKNPHKI
                     NLFIPMKNKKTFSFLKRRYDYKVKKIEKFSNSTTEFYREHNLNFQSVPQELFYLYGYA
                     NTQLIWAINPLGLYKLAESIKFTDEMIKKFTGEPKVIEHSKNVKVNNPNPTPPDNFAF
                     VKKQLEWLGLENTFNEANLINLDSEIVDSKEITILKNYLDKNLDKKLYKDEQQELSNF
                     ITEELKTVYTKKDYRTKKLNLKTINKILLENNLLYKIERSKGCSYWIIYKS"
     gene            complement(2690..3262)
                     /locus_tag="ACGI68_003472"
     CDS             complement(2690..3262)
                     /locus_tag="ACGI68_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0139875.1"
                     /translation="MSTTRPLEVEEYNQILNLLDTGFTYTNDDGKQKIFRPNIKVKLA
                     LILEANLGLRISDILRLQVKNFKNDKLEITEKKTDKLQYRDINSAITNLIKDYAILNK
                     LSPNDFLIDIGERAIQKQLKIMCNFLNIQNISTHSFRKMYATQQFKNNNNNLYLVKEL
                     LNHTSIATTQRYIRVSQKDIDKASKDFLLI"
     gene            4006..4290
                     /locus_tag="ACGI68_003473"
     CDS             4006..4290
                     /locus_tag="ACGI68_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFH0139876.1"
                     /translation="MAGYRDNWFDNNDSKHGWYTCVRCGKKLRKGNVDIDHIIPQKYG
                     GKDNLNNLQCMCKSCNRSKKDSLNNTVTDYSKNTIKNTKKTLDGLFKKLK"
     gene            complement(4611..5471)
                     /locus_tag="ACGI68_003474"
     CDS             complement(4611..5471)
                     /locus_tag="ACGI68_003474"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0139877.1"
                     /translation="MKNEYIIKKSNYFIMNCKYDLSIQEQRIILTLASMIKPTDEEFK
                     EYKFSTKEFKELLNLKGQSAYSEIPKITKKLLGKIIEIEEENEIIQCAWISTAKHLKK
                     EGVIILKIASDLKPYMLQLNTLYTSYKLKNVLSMKSKYSIRIYEILKCHEFKQQGFID
                     ISILELRKLLKAEHIYPKYANFKQKILEVVKTELEEQTDISFEYEEFKTGRTITNLRI
                     FINKKDVIVNNFKEECVTKVKEQTKTKKIIGDMPRQKTKFHNFNETFTQYTNDELDDI
                     IKKSQKEKFK"
     gene            complement(6078..>6257)
                     /locus_tag="ACGI68_003475"
     CDS             complement(6078..>6257)
                     /locus_tag="ACGI68_003475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139878.1"
                     /translation="MSLIISNIMLQTYILYKIFTFIDNMQLKFPTFTNTFLCYSKPFK
                     WLPEAFKLDLCIILT"
BASE COUNT         2189 a          754 c          656 g         2658 t
ORIGIN      
        1 aaaaaaaaca agcttcaaaa ggctttttag aagcttgttc actccttaga ttgatttcat
       61 gtatgcttaa ggtaaatcca ttaagaccac tcataaaaac cttctaaaaa gccttttgaa
      121 gcttgtagga tatgattaag tacattactt atcataataa ccatttatta cttttgaaat
      181 agtacatttt gaccattttg ttattttttg tatttttgaa tagcttaaac catcttgtga
      241 taacttttta atcttgtcaa tatctttttg tgttatttgt tgtttttttc tttttggtat
      301 aaaagcttta gtcttaaatt cctctaattc ttctctatat attttaataa tgttttttgc
      361 ttttttattt agatatttta aatactttat atctttattc aaggtatcta tagtgtcatt
      421 tgtttttgta atttcactat ttagaatttc aatttcatta agtaatattt ctatatcttt
      481 aatagtaaaa ctatccactc tggtatttaa aaattgcttt tttttaattt catatttggt
      541 tataacttca ttaactttat tgtccatatt gcagcctcca aaaatcgtta tttattaatt
      601 acgtaactag aataacaaat aacaaatgag attacaaatt taattttttt aaacttgaat
      661 atataagtta agacttgtat attatccaat aactacaacc ttttgacctt tcaattttat
      721 ataaaagatt attctctaat aaaatcttat ttatagtttt cagatttaat tttttagtcc
      781 tataatcttt tttagtatag acagttttca actcttctgt tataaaatta gatagttctt
      841 gttgctcatc tttataaagt tttttatcaa gatttttatc aagataattt tttaatatag
      901 taatttcttt tgaatcaact atttcactat ctaaatttat taagttagct tcattgaagg
      961 tattttctag ccctaaccat tctaattgtt tttttacaaa agcaaagtta tctggtggtg
     1021 ttgggtttgg attattgact tttacatttt tgctatgctc tataacttta ggctctccag
     1081 taaatttttt aatcatttca tcagtaaatt ttatagattc agctaactta tacaatccta
     1141 gagggttaat tgcccaaatt aattgagtat ttgcatatcc atataaataa aataattctt
     1201 gtggaactga ttgaaaattt aaattgtgtt ctctataaaa ttcagttgtt gaatttgaaa
     1261 atttttctat ctttttcact ttataatcat atcttctttt taaaaatgaa aatgtctttt
     1321 tatttttcat aggaataaat aagtttattt tatgaggatt tttaatgtct actctaattc
     1381 taccaatttc ttgaataaat gtagttttgt caagttccat gactactaaa ttcctaatac
     1441 tcttgtcttt taaattaatt ccattgtcta atactttagt tgatatgagt acatcacatt
     1501 caaaactgga gttatgtact atattctctg cttccttagt gtttgaacca ctcttgaata
     1561 tttcaatgct tattttttca ctttttaaac gctcttttat atactgtgca tctttaattt
     1621 tagaaacaaa aataagccat ttatctttat ttgctgaaaa atgatttttt aaaagaataa
     1681 taatatcttc aatattttta aaatatttta catctaaata actataatct ctaccagtag
     1741 aaaactcata ctcattattt aaatttgttt tttctttaaa attatttgta tgattaagag
     1801 aaatgtattg attaaaaaaa gtataaatgt tgaaattaca attatttttc cttgcttctc
     1861 cccttgaatt ttctaagcga gcattaaaat ctattgcatt tttaagttca tataatgttg
     1921 cacttagaaa tattctaaat gagttactat attcttttaa aactaactca ttgaatataa
     1981 ggttacattt tgtattgaac tctaaatctg ataacataaa atgacattca tcaaaaacta
     2041 tgaaatcaaa gaaatttaga ttatttttgt gttgattaca gtaaaaatta ttataatata
     2101 taatttcccc taactgttga tatgacataa ttgtaatgcc atttgttatc tttgacagct
     2161 tatctaaaat ttgaaatgtt tctttaatat tattttcagt attatcacta ttacaaattt
     2221 ttttaatgtc cttatcaaat tcatataaat ctaaatttga ttttaatagt tctagtttta
     2281 cttgtctttt caaattaatt ctattgcaga taattaaaag atttaatttt cttttgtttc
     2341 ttatactttg attaactagt gggacaattt tatcaattat aaattttgtt ttgccagttc
     2401 cagtttgtgt ttcaataaaa acgttatttc tattatttat aatatgtcta tcttccatat
     2461 tccatttttt ataatcttct ccaataatat ctgtaaccca ttctaaattt aatttttcca
     2521 ttatataaac tccatgattt taataattat ttgcaaatga tatgataatg ataatcctat
     2581 aaaaattaat aaataataaa tatagtatat attcaaattc atattactat ttcactatat
     2641 catagagatt attatttttg aaaaattacc taaaaaatat ctgaaatatt taaatcagta
     2701 aaaaatcttt agaagcttta tctatatctt tttgtgatac tcttatgtat ctttgtgtag
     2761 ttgctattga tgtatgattt aataactctt ttactaaata tagattatta ttattatttt
     2821 taaattgttg agtagcatac attttcctaa aactatgtgt tgatatattt tgaatattaa
     2881 gaaaattgca cattatcttt aattgttttt gaattgctct ctccccaata tcaattaaaa
     2941 aatcatttgg agatagttta tttaaaatag catagtcctt gattaaattt gttatagctg
     3001 aattaatatc tctgtattgt aacttatctg ttttcttctc tgtgatttct aacttatcat
     3061 ttttaaagtt tttgacttgt aatcttaaaa tatctgatat tcttaaccct aaattagctt
     3121 ctaatatcaa agccaattta acttttatat ttggtctaaa tattttttgt tttccatcat
     3181 cattggtata tgtaaatcca gtatccaaca aattcaaaat ttgattatat tcttcaactt
     3241 ctagtggtct tgttgtagac atttttaaaa cctccataat agataaatat tttatataat
     3301 aatactatta tagcacagta gttcgtgttt tatgataaat aagttcgtat tattgtttgc
     3361 ttatttttaa atttttttga aagtattgca attactaaaa ttcctgcaat agttcgtgtt
     3421 ttatagaaaa tacgaactat ttagttttgc aatttttttg gcatcataca cctaaaaatt
     3481 ataatctcta tacctatttt ctatactgca aggaatagaa aagggaggtg tatatatgct
     3541 taaaataata ataagattag tgttattatt agtcgagata tatttatatt taagataata
     3601 ttttaaaaca tattttatat tttaaaataa aagtctaaag gtgtcggaac atcataataa
     3661 aatttattat tatatttata tacatttttc ataaatgtat tcgttcctca tgtgtcggtt
     3721 taagggaggg tatgtctttt ttaaaaaaag acttttttat aataaatttt actatagtaa
     3781 ttttcttagt aaccaaaata gattttgaat taaatatagt aattattact atatctatca
     3841 tatttcctgt atataattaa ttaaaattaa gttactacta gaaacaataa gagtattttg
     3901 tataatgtat atcaaattac tatagtagat tttaagatat ttagaggtaa atactgaata
     3961 tttgtctaat tatatttaaa aaataaaaag taggtgaata aaataatggc aggatataga
     4021 gataactggt ttgataataa tgattcaaag catggttggt atacatgtgt tagatgtggt
     4081 aagaagttaa gaaaaggtaa tgtagacata gaccatataa taccacaaaa atatggtggt
     4141 aaagataatt taaataactt acaatgtatg tgtaaaagtt gtaatcgttc aaaaaaagac
     4201 agtttaaata atactgtaac ggattacagt aagaatacaa taaaaaacac taagaaaaca
     4261 ttagatggct tattcaaaaa actaaaatag agatactcaa atgagtatct ctattttagt
     4321 tttttattgt taaattttat aaaaatctta ttctataggt ttaataattt cttcaagatt
     4381 gcctaactta taaccaaaat tatcatctga tttattttca attttgacta ccattaatat
     4441 tttaatttga ctctaaatct ttaatcaatt ctacatttga cttttcaatt tcattcaaaa
     4501 tagatgtatc tgcacttata ccttctttat caaattgatt agatacaatt tgatacatgt
     4561 ttttatataa aaaatacgaa aatatggcta actaacttag taagttagat ttacttaaat
     4621 ttttcttttt gactcttttt gattatgtca tctagttcat catttgtata ttgagtaaat
     4681 gtttcattaa agttgtgaaa tttagttttt tgtctaggca tatcaccaat tattttttta
     4741 gttttagttt gttctttaac ctttgtaaca cattcttctt taaaattatt tactataaca
     4801 tcttttttat ttataaaaat tctcaaattt gttatagttc ttccagtttt aaattcttca
     4861 tactcaaagc taatatctgt ttgttcttct aattctgtct ttacaacttc aagtattttt
     4921 tgtttaaagt ttgcatattt aggatagata tgttctgctt tcaataattt tcttaattcc
     4981 aaaatactaa tatcaataaa accttgttgt ttaaactcat ggcattttag tatttcataa
     5041 attctgattg agtacttact tttcattgat aaaacatttt ttagtttata gcttgtatat
     5101 aatgtgttta attgtagcat gtaaggcttt aaatcggatg ctatttttaa tataataaca
     5161 ccttcttttt ttaagtgttt agccgtagaa atccatgcac attgtattat ttcattctct
     5221 tcttcaattt caataatttt tccaagcagt ttttttgtta tctttggtat ttcagaatat
     5281 gcagattgtc cttttaaatt taataattcc ttaaattcct ttgtactaaa tttatattct
     5341 ttaaattctt catctgtagg ttttatcatg gatgctaatg tcaaaattat tctttgttct
     5401 tgtatagata aatcgtattt acaattcatt ataaaataat tagatttctt aattatgtac
     5461 tcatttttca tacatacacc cctataaaat tataatctat aactataacc tagtaaagtt
     5521 atagttagca ccaaaaaagt tatatttcgc accaaaaaag ttatatttcg caccaaaaaa
     5581 gttatatttc acaccaaaaa agttatattt cacaccaaaa aagttatact tagaaaatat
     5641 ggacaagtaa ttccaacggt ttaatacccc tctaaaatag tttaaaataa ttaaaatatt
     5701 tatttaaaat aataaaagac aaaaaataaa aaataaaaag aaggattttt ttagtgcaaa
     5761 tggaaattat acagtaccca aattaaaaaa cattaaaaga catttataag tttgacaata
     5821 ttctataaaa actcataagg ggtgaatagg atgacaccta aagttttaga catgtttata
     5881 acgaacaaca tttataggtt tgaaagttgt ttttttctag ttgttaattg ttaaatacaa
     5941 ctttcagact tattatttaa atataaattt gaagaagcta taataaatca taattttata
     6001 agactatttt caatatacat aaacctatat tgttactaaa taccccatag aatcatttct
     6061 aaggggtatt tatacgttta tgtcaatata atgcataaat caagtttaaa cgcttctgga
     6121 agccatttaa atggcttaga atagcatagg aatgtgtttg taaaagttgg aaattttaat
     6181 tgcatattat caataaaagt aaatatctta tatagaatat atgtttgcaa cataatatta
     6241 cttataatca aggacat
//
LOCUS       DAYASV010000178         6140 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYASV010000178 DAYASV010000000
VERSION     DAYASV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.183"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(66..695)
                     /locus_tag="ACGI68_003476"
     CDS             complement(66..695)
                     /locus_tag="ACGI68_003476"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HFH0139879.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALRQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(774..1736)
                     /locus_tag="ACGI68_003477"
     CDS             complement(774..1736)
                     /locus_tag="ACGI68_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139880.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKVKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVIEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(2498..6013)
                     /gene="cbpA"
                     /locus_tag="ACGI68_003478"
     CDS             complement(2498..6013)
                     /gene="cbpA"
                     /locus_tag="ACGI68_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HFH0139881.1"
                     /translation="MQQKCKKKALSMILSISMGLGPVASFNASNTFADTIEESTNAGN
                     AEGGTNAGNAEGDTNAGDAEGDTNAGDAEGDTNAGDAEGDTNAGNAEGDTNAGDAEGD
                     TNAGNAEGDTNAGDAEGDTNAGDAEGDANAVVVEEEATAEEPTSVGDFKISGGEINVD
                     FKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFDGVNIKTASNSPFTL
                     TPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLIIDDSVVNRDIEGEFIVP
                     EGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYYSAGIGGGNTESGGNIII
                     NGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGIITAKGAYHGAGIGGGYYTT
                     TKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFGDGCVNPGTNRGYRILVT
                     GGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLGGAVAYGDLEKKTKVFMN
                     KISLTYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDAQGILYLWFSDQFKGSEVSV
                     NLVVIDKITGEVLKPDDFYIKDIGSGSQFLKQYEEFTIDDTPPYLIIKRYDGLPFEED
                     VEKSFLDWIFKDGGIPIITPKGEKLTDKKFMTISSQRLNEETMEPDINLGSDDLTKQV
                     DAGKYQLSITSTQFSSIYPFKDAYWGHRAYYKAEIIPADTETSLVVEESKAKTDVFKP
                     TDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVELKTHNKGSESHNYSTAS
                     IEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLKLDMTNVDVDGDGIPDIN
                     IDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNGDGIWKPDKNIDTNGDG
                     NPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPNLNKPDPDNGGNSKPDSG
                     NGGNSKPDSGNGGNSKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPD
                     PDNGGNSKPDPDNGGNSKPDIDTDGDGKPNINIDTDGDGKPDINIDTDGDGKPDINID
                     TDGDGKPDINIDIDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQSNIM
                     LDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT         1824 a         1203 c          671 g         2442 t
ORIGIN      
        1 ttgtaatttt ttgttcttta tatcacaatg ctataccata ttaaattgaa aaataagttt
       61 taaatttatt tgttacaatt cattaattca aaaaaatatt ttgagtcatg agttttgtaa
      121 agatttagcc atgaatttaa tgcttctgat tcaaaaacat ctaaaagttt caatacttga
      181 aacgtaaatt ccaacggagc aattcctgaa gccgtaacta aattttcatc actaactaca
      241 gattccgttt tataatattt ttctccagag taatttggta ttaccatttt aagaaactct
      301 aagtcattac ttgtatgatt tctaaaatct aatagccctt ttttagctag gcctaatgta
      361 gctccacata tggctgcaac tatagaatct tgtcttaaag cttctttaac tttttctaat
      421 attatctcat gattcataga taaccaagta tttccccctg gcaaaattaa taaatcctta
      481 ctttccatat taaactcatc aactgtaatt tcaggtaaaa cttttaaccc acccattgtt
      541 ataataggat ttttatcaat tcctacagta attactttta gtggctttaa tcctttttta
      601 aaatatcttc ctgtatttaa ttcagcagtt aaatatgcca cttcccaatc tgacatagta
      661 tcaaatacat acatatatac ttttctattt ttcatctttt ttactcctta tgtaattaat
      721 ttatatatct aattataaaa taacttaact gacatattca gtcagttaag ctattaaatt
      781 tgataataat caattaattc tagtaaatct tcaattattt tcttcctaag gcttagtggt
      841 tcaattactt gtattgtctt tctataaggt aaaagtatat ttggtatgta cttgcatatt
      901 acttcctctt caagcataaa tattatttta ttaggtgttc tttctttcaa gtagtgccct
      961 aaaaaccaat gttggcttaa actatctaaa gaatctatac tcccttctat aactaattga
     1021 attatatttt gttcatcttt tatttcaggt ataattcctt ttataaaaaa ttcacttgct
     1081 gaaaattctg tcggtctgtt aaatgtattt gatgtttcta tgatttcact aatcctatcc
     1141 actctgaaac tacgaacctc atttctcata ttacaaaatg caactacgta ccaattatta
     1201 ttccagtgaa caattccata tggatttata attctatatt ttgattttac tttacgacct
     1261 gcatgatagt tgatttctac agatagctcc tttaatatac tattctccaa ttttttaagt
     1321 actgattcta tagacacttt cccaattggt tttattactt caagggctct taagtggttt
     1381 gttaaaatat cttcttgttt ttgatttgaa tacattttta actttaatat cgatttatct
     1441 aatgcttcgt ctaagaagta tccttctttt ttagcaaata gtgctgcatg aagtagagat
     1501 ttttgctctt ctatatcaaa aaaaagaggt gctttagtaa aattatttaa aaggctatac
     1561 ccaccattat gacctgtatc tgaaattata ggtactccac tcgcagaaag tacatcaata
     1621 tatcggtaca ctgttcttat atttatttct aatttttcag ctatctgttt tgctgtaatt
     1681 tttttgtcag agtttaacat ccacagtata gacatcatat tgtcaatttt agccatgtag
     1741 tttcacctct ttctatttgt tatataaagt tattatataa ctttttgatt gattttttac
     1801 aaaaaaacat tagtaaactg ctaataattt atactaagat ttatttcaaa tatttataga
     1861 tagtcttttt ctatagttaa gttatccatt aaattacata gtagtatatc tattttagtt
     1921 gatatatttt cagctacctc ccttgaatat tattacaata agtctaaatc ttctttcttc
     1981 cataaatata ttatgaggaa agactatcat ctttaatggt ttaatagtat aaaagatatt
     2041 aatttaaaaa aatctatttt tatatcagat gggtattgtt ttttataaaa ctgaaaaaat
     2101 cacctaatag aatctatgat tttttaacat aacctccata aattagttcc aattatcact
     2161 tcaaattcaa atttttatct gaagaatata caatcatatt tccatactca acataatatt
     2221 gagtgtttgg cttgtattta caaattatta ttctaacaaa aacaactata catagattaa
     2281 aatcatcttc taaacataac tactaaaatt gaattaatga tatcaattcc attaatctct
     2341 tttaataaaa aattctcttt tctatatgaa aattatttca aaacttttaa gttaaataca
     2401 aaaatgacta taaatagatt tttatttaca caaatctatt tatagtcact atagtttatc
     2461 caataaaata ataaaataaa tatgtacaca ataaatgtta tttacgtctt aagtatttat
     2521 ttgttagatt tttaattaaa aaaaacaagc taatgaacat taatgctaaa tctagcatta
     2581 tattgctttg atctcctgtt tgtacatttt gacctacatt actatctaaa ctttcatctg
     2641 tatctatatt tatatctggt tttccattcc catctgtatc tatatttata tccggttttc
     2701 catctccatc tatatctata tttatatctg gttttccatc tccatctgta tctatattta
     2761 tatctggttt tccatctcca tctgtgtcta tatttatatc tggttttcca tctccatctg
     2821 tgtctatatt tatattcggt tttccatccc catctgtatc tatatctggt ttgctatttc
     2881 ctccattatc tgggtctggt ttactatttc caccgttatc tggatccggt ttgctatttc
     2941 ctccattatc tggatctggt ttaccattgc caccgttatc tggatctggt ttgctatttc
     3001 ctccgttatc tggatccggt ttgctatttc ctccattacc tgaatccggt ttgctatttc
     3061 ctccattacc tgaatctggt ttgctatttc ctccattacc tgaatctggt ttgctatttc
     3121 ctccattatc tggatctggc ttatttagat ttggattacc tgtatcataa cctcctgagc
     3181 cagtatctac atttttatca ggcttccaat aatcatctac tccatcatta tctgtatcta
     3241 ttgctggacg attataatct gtatcagggt ttccatctcc attggtatct atatttttat
     3301 caggtttcca aataccatct ccatttccac cttctgtact aggcttccat tcacctgtat
     3361 tatcagtatc tatgttaata tctggcttcc aatcaccact cgtatctata ttgatatctg
     3421 gccttccatc accatcagtg tctatattta tatccggtat tccatcccca tctacatcta
     3481 catttgtcat atctaacttt aacttctttt catcctcact accttcaatg taatttatat
     3541 catctccaat atattttaca gtaatagttt gttcagtagt aaaaatatga tgattgctat
     3601 ctaaaggtct ccattcaata gatgcagtac tataattatg actctcacta cccttattat
     3661 gagtttttaa ttctactgga tctttatatt cctttccatt aatatagaat tgtacgtaac
     3721 ctcttggaga ggcacaatct ggtccttctt tcttatctgg tgaaatagta acatttaatg
     3781 taaacgtatc tgttggttta aaaacatcag ttttagcttt agactcttct acaactaatg
     3841 aagtttctgt atcagcagga ataatttctg ccttataata tgctctatgc ccccaataag
     3901 catccttaaa aggatatata ctcgaaaact gtgttgatgt aatgcttaat tgatatttcc
     3961 cagcatcaac ttgttttgta agatcatcac ttcctaaatt tatatctggt tccatcgttt
     4021 cctcatttaa acgttgagaa ctaatagtca taaatttttt atctgtgagt ttttcacctt
     4081 taggagtaat tattggaata ccaccatctt taaatatcca atctaaaaat gatttttcta
     4141 cgtcttcttc aaatggtaat ccatcatatc ttttaataat caaatatggt ggagtatcat
     4201 caatagtaaa ttcttcatat tgctttaaaa attgagaacc tgaacctata tctttaatat
     4261 aaaaatcatc tggttttaga acttctccag taattttatc tataacaact aaattaacag
     4321 atacttcaga tcccttaaat tgatcagaga accataagta aagaatccct tgagcatctg
     4381 tataactagg tgctccataa ggataaattt ttccatcaat tagcatttta aaatcatcaa
     4441 ctagtgtagt actaacttta tcttgcccta aatacgttag agatatttta ttcataaaaa
     4501 cttttgtttt cttttctaaa tcaccgtatg caactgctcc acctaaactt ttgaatgttg
     4561 ttgcttttaa tgaaccacct gttactataa catctcccgt tgtagcactt aggtcaaaaa
     4621 tccaatctcc cactcctgtc atataaggta aaatcgttcc tccagttaca agtatacgat
     4681 atcctctatt tgttcctgga ttaacacatc catccccaaa tgcactacca tgtgcacctc
     4741 catatgaata ggaaagtcca ccattaattg taatattccc actataacca gaacctgtgc
     4801 taggatctac tactgaggga cttaatgcat tctttgttgt tgtatagtat cctccaccta
     4861 ttccagctcc atggtatgca cctttagcag ttataatacc tccattgatt gtgatatctc
     4921 caccactacc attaccgcct ccacctattc cagctccata tcctgtatta cgactcgatt
     4981 cttgacctat agcagttata gcaccaccat taataataat atttcctcca ctttcagtat
     5041 ttccaccacc aatacctgca gaataataat ttccaacaac ttttaaactt cctctattat
     5101 tactatctag tagtgtaagg cgatctcctt tttttaactt gtctccattt tctagagtta
     5161 catctctagg aatttttcca ccctcaggaa ctataaactc tccttcaata tctctattta
     5221 caacagaatc atcaataatt agagtagtac cttttcctgc tctcatacct ggataatata
     5281 catcacctga ctctagcttg ttttcactac catcttgtaa tataacatgt gcatatgcac
     5341 caccatcttt atctggtgtt aaagtaaatg ggctattaga tgcagtttta atatttactc
     5401 catcaaatat taattcagct tttacatctt taggtataac aatcctatgt gttgttggag
     5461 tatttggatt gttattttga attgtcattt gttttccact aagaatagtt aaaacgccac
     5521 ttgcaaattt aaaatcgaca tttatttctc caccactaat tttaaagtct cctacagacg
     5581 taggctcttc tgcagtggct tcttcttcta caactactgc attagcatct ccttctgcat
     5641 ctcctgcatt agtatctcct tctgcatctc ctgcattagt atctccttct gcatttcctg
     5701 cattagtatc tccttctgca tctcctgcat tagtatctcc ttctgcattt cctgcattag
     5761 tatctccttc tgcatctcct gcattagtat ctccttctgc atctcctgca ttagtatctc
     5821 cttctgcatc tcctgcatta gtatctcctt ctgcatttcc tgcattagta cctccttctg
     5881 catttcctgc attagtactt tcttctatag tatctgcgaa agtatttgac gcattaaatg
     5941 atgctactgg tccaagtccc attgagatac ttaaaatcat tgatagtgct ttctttttac
     6001 atttttgttg cataaactcc ccctcccctt aataattttt aatatacact gtatacatcc
     6061 ttatatacac tctaataatt cttaatatac gctgtataca ttctaatgca cactctaata
     6121 atttttaata tatacttaaa
//
LOCUS       DAYASV010000179         6124 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYASV010000179 DAYASV010000000
VERSION     DAYASV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.184"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            119..769
                     /locus_tag="ACGI68_003479"
     CDS             119..769
                     /locus_tag="ACGI68_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFH0139882.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(909..2285)
                     /locus_tag="ACGI68_003480"
     CDS             complement(909..2285)
                     /locus_tag="ACGI68_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFH0139883.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2301..4214)
                     /locus_tag="ACGI68_003481"
     CDS             complement(2301..4214)
                     /locus_tag="ACGI68_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0139884.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4350..4982
                     /locus_tag="ACGI68_003482"
     CDS             4350..4982
                     /locus_tag="ACGI68_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFH0139885.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5121..5660
                     /locus_tag="ACGI68_003483"
     CDS             5121..5660
                     /locus_tag="ACGI68_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFH0139886.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FQMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5836..6006)
                     /locus_tag="ACGI68_003484"
     CDS             complement(5836..6006)
                     /locus_tag="ACGI68_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HFH0139887.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2263 a          856 c          859 g         2146 t
ORIGIN      
        1 gtagaatgaa tttgtcaagt ttaatttagt tgtaatttta tcttttatta aaaataatat
       61 aaattaaaac atataaataa aatattttat acaatatatt gtaaaggagg taactttgat
      121 gaaagcagaa aaaacaagta tatatccaat tattaaaaat aatttaaaca aaatatcaat
      181 tgttgaatat aaaaagggtc aaagatttgt agcatcagat aaagacttac aagaagtgtt
      241 ctttataata gagggagttg cattagtgga gtgtactact agaggaggaa ataaatttct
      301 tgttgacatt gtacctgaaa atgaatttat aggcaaaatt agttacatat atgaacataa
      361 tttgaaatgt gatatatttg caaagaccaa tataaaatta tttcgatttg aaaaaaatgt
      421 atttgaagag ttttatctca aaccagattt tattgcacta tttcacagaa aatgtacaag
      481 aagaatatat gaattatata aaaccagaat ggtaagagaa ctattttctt gcacagaagt
      541 tattgcctat tgtatatcaa ataaacaaga aaataatgtc tgcaatataa aagctattcg
      601 taatttcagt gaaatctatt caattagtag aaaaagtaga taccattctc tgctaaagtt
      661 aactgaaaag gagatagtaa aaaaaatggg taatacatat gaaattttaa actatgatga
      721 attacataaa ttggcatttg aagtaaaaga atttttggaa gatgagtaaa aattgttact
      781 taatcaaaat aaagtataat aatttctgta aaaaatagaa atagtctcac tgaaaatcaa
      841 aaatacttta gttattttca atgagactgt ttagaaatat tgtatactta taatgtatag
      901 tataattatt aatcaatttc ctttactata ttaatagccc attttttaga taatgctcta
      961 ggaacaccaa atataaactt aaccacttct tcactataag tgagcaaaac gagtacaaat
     1021 ataggtgcac ctttaaatgc agctataaaa gttagaggaa tagaagctaa ccacatacat
     1081 cctaattcta aaaatagagc atatttagta tctcctccac ttcttaaaat accaattata
     1141 accaatgtat taaaagattt aagagccatt agtattccca ttataaagaa tatcttagtt
     1201 atatcagaag ctaagctatc agaaacacta aacattttta ataataatgg tatatttaaa
     1261 attaaaataa ttccaagtat taaaccagct gaaaatacaa gtatagaaaa ctttttagca
     1321 tattcaatgg ctcttttaat atggtcagct ccaagttcat ttccaagcat aatagatgca
     1381 ccagatgcaa tacatacagc tgtcattatg aagaaattac ctgtactggt tgctatctgg
     1441 ctagctgcta tagcagaagt accagctctt gcatatgcaa cagaatacag gacagttcca
     1501 attgcccata cagagtcatt tacaaatata ggaaagcttt ttgatgaaaa agattttata
     1561 aaaattttat caatagcttt tagatttttt agaccaaact ttaatatata gtctttttta
     1621 taaaggtaaa cataagtaat cattaaaatt aattcacata ttctagcaat aacagtagct
     1681 agagcagcac cttttacacc caatgcagga agaccgaagt taccaaatat aaatccataa
     1741 tttaaaataa catttgttac aagtgcaaat gcactacaaa tcatacctaa cttaggattt
     1801 ctaacacctc ttgaaccagt gctaaataca gtacttacag caattaatgg ataactaaat
     1861 actgcaatac taaagtaatc tatacaaagt tttaccacct cagaatcata cgaaaatatg
     1921 tgtataattg gctttggtgt tagtaaagca ggaataacaa atacaaaact taaaacaatt
     1981 gccaatacac aagtaagacc tgtgactttt cttatattac tcacatcctt ctttccataa
     2041 aactgtgaaa taaaaactcc agcacctcct gtgataccag ataaggacat attaaaaaga
     2101 aagaaaaatt ggtttgcaac tccaactgaa gctacagatg tttcccccaa actacttatc
     2161 ataattgtat ctattacatt tacagaagta gaaattaagt tctgtatgat aataggtata
     2221 cataaagata gaagtatttt ataaaatctt ttattttcgt cttttaaaga aaatagttct
     2281 tgcaaataat cctctccttt ttacataaat tcttcccact catcatatag agaggcaagg
     2341 ttattttcta aattaatttt ttcttggcta acttctttag ctttatctgg attagagtat
     2401 acttcttctt gacataaaag tatatctaat ttttcaattt caatctctaa cttttcaatt
     2461 tctttttcta tattttgtct ttttattcta tttttctttt ctatttctct ttgttctctc
     2521 tcttttcttt tttcatcttt tatttgagtt ctagtttttt cttctttttc ttctataata
     2581 ctcatctctt ggatttgcct ttttttatct atataataat cataatttcc cagatattca
     2641 gtaattccat tttcatctaa aactaaaatt ttatcaacaa cagtatttag aaagtatcta
     2701 tcatgtgata ttgtaaatat agtcccatca taattagtta atgcctcttc aaggacttcc
     2761 ttagaatcaa tatctaggtg attagtaggc tcatctaata acaataagtt tgcatttgat
     2821 agtataagtt taagtatggc aactctagct ctttctccac cactcaatgt agaaatcttc
     2881 ttaaaaactt cttcatctac aaataaaaat gcccctaaca tatttctcaa agtagtttga
     2941 gtaagatgag tattattttc ccaaatctca tcaattattg tgttatctaa attaagagtt
     3001 ttttgttcct gatggaagta ggaaacatgt acattagttc caaatttaat attgccacta
     3061 agaggttgta gttcattagt tattatttta aataatgtag atttgccaac accatttgca
     3121 cctataagtg caactttttc ccctctatat atatctaaat ccaagtcttt aaaaagtatt
     3181 ctttctccat aacccataga tacatctcta atttgaagaa catcattacc actctggaca
     3241 gaaggcgtaa attgtatacg agcttttttc ctataagctt ctggtttatc taaaacgtct
     3301 accttgtcta atgctttttc tttacttctt gcacgcttta ggtgtttttc tctaccataa
     3361 gcttttagtc tttcaattga ttcttcttgc ttttttattt ctttttgttg gtcttcaaat
     3421 ttcttaagct ctagttcttt ttctatttta gataactcaa caaatttaga gtagttacca
     3481 ttatatactt taagtttttt gttgtgaact tcaaaaattc tatttacgga ttggtctaag
     3541 aaatatctat catgagatat taacatgaca gtccctttat attgttttaa aaatacttct
     3601 aaccattcta ttgcttcaga gtctaagtgg ttagtaggtt catcaagtaa caatagagtt
     3661 ggtttcttaa gaagtaattt tcctaaaaga actctagttt tttcaccacc agaaagtatg
     3721 cttattggct tatccatatc attgtcacta aatcctaatc cctttagaac tcctttggct
     3781 tcagatttat atccatatcc atttaaatct gaaaataatt ctagtttatg tgaatattca
     3841 tccatgattt tttctataat tggagagttt gtttttgtac tttcttcaga tattttaacc
     3901 tccaattctc ttaaatagga ttccatttct atcaaatttt taaaaacttc tagtacttct
     3961 tccaaaatag tattatccga ataaaaattg gtattttgtt ctaaatatcc gatttcacaa
     4021 tctttagaag tgtaaatatc tccactatca tatccataaa ttcctgaaat tattttaaat
     4081 aaagtagttt ttccagtacc attaacaccg attataccaa ttttatcgcc ttcatttact
     4141 gtaaaactaa tattttctaa tatagaatct ataccaaaac ttttatttaa gttattacat
     4201 gacaaaacaa tcataaattt ctacctcctt gaactatcta aaattataat gttttgatat
     4261 aatataatca aggttatatg tattagtttt gaaaaagaaa tattttattg tgaatcaaac
     4321 aaaaaagagg acacttttag gaggtgaaga tgttgggaaa taaaaatata tcaatggcag
     4381 ttataagaag gctcccaaaa tatcatagat atcttggaga cttattagat agggatatac
     4441 aaagaatatc ttctaaagaa ttgagtgata taatagggtt taccgcttct caaataagac
     4501 aagatttaaa caactttggt ggatttggac aacaaggata tggttataat gtagaagctc
     4561 ttcatactga gataggtaaa attcttgggt tggatcgacc atacaacgca gttcttgtag
     4621 gagcaggtaa cttaggacaa gctatagcca attatgcagg atttagaaaa gctggattcg
     4681 agataaaagc tttatttgat gcaaatccta gaatgatagg tttaaagata agagagtttg
     4741 aagtattaga ttcagatact ttagaagact ttataaaaaa caataatata gatattgctg
     4801 tattatgtat acctaaaaat ggagcacaag aagttattaa tagagttgta aaagctggaa
     4861 tcaaaggtgt atggaatttt gcacctttag atttagaagt tccgaaaggt gttatagttg
     4921 aaaatgtaaa cttaacagaa agtttattta ccttatcgta tttaatgaaa gaaggaaagt
     4981 agacaactta aacatattaa taatgagcag ttgtgaagag tattgagaaa gtcagaagat
     5041 ttaatattaa atcctctggc tttttgaaat attaggataa aaatttaaat aatgtatgga
     5101 aataaacaat attaaggaga gtggtttttg tggaggaatt atggaaaaag gcaattgaat
     5161 ttcatggtca tgaatgtcca gggttagcta taggatttaa agcagcactt gcagcaagag
     5221 aatatttagg atgtaaacag tcaggagatg aggaaattgt atgtatttct gaaaatgatg
     5281 cttgtggaat agatggtata caagtcgtgc taagttgcac aatgggtaaa ggaaatttac
     5341 tacttaggat gactgggaaa tcagtatatc atttctttaa tagggaaaat ggaaaatcta
     5401 taagaatata tgtaaagtca aagccatttc aaatggatag agaaggatac aaaaagtact
     5461 tattagaaag tgattttgaa gaaatatttg agattacaga tactaagtta agacttcctg
     5521 aaaaagcaag aatatttaaa tctcttaaat gtgaagtgtg tggtgaattt gcatctgagc
     5581 aatatataag gattgaagaa ggaaagaaag tatgtcttga ttgctttaat aaatatgaaa
     5641 gattttatga ggagtattaa aattataaat tagattaaag ttaaaagtat tagatatgtg
     5701 ctaacagtat tttaagatga attaggtagc aacataataa agtatataaa taatattaat
     5761 tgaatcatac ataattaaaa agaggctgat tagtatttat aaaataaaat cagtctcttt
     5821 atgaataacg aattattatt ctggttgagg tgcatctaca ggacaagctc ctgcacaaga
     5881 accacagtct atacaagaac cagcatctat aacatatgcg tcgtctccag ctgatataca
     5941 gctaacaggg cattcagcct cacaagcacc acagcttata caagcgtcag taattttata
     6001 tgccattttg aaatcctccc ttttatatgt attgtaattc aataacatta taacaaatat
     6061 tattgaatat aacaattaaa ttaattctac aacaaaaact ttattatgat attcttgttt
     6121 taaa
//
LOCUS       DAYASV010000180         6020 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYASV010000180 DAYASV010000000
VERSION     DAYASV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.185"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            81..1370
                     /locus_tag="ACGI68_003485"
     CDS             81..1370
                     /locus_tag="ACGI68_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFH0139888.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     gene            1424..2503
                     /locus_tag="ACGI68_003486"
     CDS             1424..2503
                     /locus_tag="ACGI68_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HFH0139889.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELCGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2524..4068
                     /locus_tag="ACGI68_003487"
     CDS             2524..4068
                     /locus_tag="ACGI68_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HFH0139890.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            complement(4231..4773)
                     /locus_tag="ACGI68_003488"
     CDS             complement(4231..4773)
                     /locus_tag="ACGI68_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HFH0139891.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(4921..5205)
                     /locus_tag="ACGI68_003489"
     CDS             complement(4921..5205)
                     /locus_tag="ACGI68_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0139892.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(5268..>6020)
                     /locus_tag="ACGI68_003490"
     CDS             complement(5268..>6020)
                     /locus_tag="ACGI68_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFH0139893.1"
                     /translation="LKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQAKKS
                     LDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVWRNIP
                     IFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPNSLVS
                     EFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDHIENP
                     RLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
BASE COUNT         2061 a          768 c         1062 g         2129 t
ORIGIN      
        1 atagttaata aatttataaa ttagaaaatt gtaaaatatg ctaaggtcag tagttttacg
       61 atttaaatgg agggatgaaa atggaagaag aaaaaattaa ctatgctctg gaacagattc
      121 cagagaatca aaaacgtggt tgggtagcta tgttttcagt tttagtagca ataggtgtgg
      181 atttatcatc tgttatttta ggcgctgagt tggcacagag tatgccaatg aaacaagcaa
      241 tattgtcagt aattgttggt tcattttttt ctgcaatttt gtatactaca tgctctttgg
      301 ttggttcatc tacaagttta tctacatcta tgataacaaa gtatgtattt ggagaagctg
      361 gagcaaaaat attttctcta gttatagggg tttctctatt aggttggttt ggagttcaag
      421 taggtttctt tgctcaaaat gcacagataa taataaaaga tatatttaat ttagatgtca
      481 gtatgcagat tttaagccta ataggtggtc tcttgatgat gagtactgca atttatggat
      541 ataaggcaat ggagaagttg agtgtgtatt cagtaccatt tttattagtt cttatgatgc
      601 taactatatt cttagctttt agagcaaatg gaatttcagt tgatgataat atgaaatcta
      661 ctatgacatt tgcaggtgga gtttctctgt caatgagtat aataatagtt ggtgcaattg
      721 tatctcctga tataagtcgt tgggctaaaa gtagaagaga ctgtgctctt tcaagctttt
      781 tgggaataca atttggaaat gcttttatga taatagtttc gatagtatta gtaaaatgta
      841 tgggaacttc agatattatg agaattttta ttacattagg aattgctata ccaggtataa
      901 tagtacttac attagcacaa tggactacaa atacaagtaa tgtatattca gcatcattaa
      961 gtatagctct tgtacttaag aaggctcctg agaaagtatt aactatagtc cttggtatta
     1021 tagcaacatt attggctgta tttggaatat atgaaggttt tataggattt ttaaatttac
     1081 ttggaattgt aattgcacct gttggtggtg tatatacagc agaatattac atagtaaagc
     1141 aagaacttaa aggctttgat aaaggagtat tatataaacc aatagttaaa cgttcaatag
     1201 tgtcttggat aattggtata ttaataactt acctttcaac atatggattt ataactctta
     1261 ctactatagc acctttagat ggttttatag cagggtttgt agtacaatct attattggaa
     1321 aagtgctttg ttcaacaaaa aataagcaag aaatagacaa agcagtataa agaaatttgt
     1381 ttataaagta taaaataaat tagaaatatg aggagaaagt aacatgagat ttttaacaga
     1441 agaaagtgta gataaaatag cagttggtgc agcagtgctt ggaactggag gtggaggtga
     1501 cccatatgta ggtaaattgg ttgcaaaaca agccattaaa aagtatggtc ctgtaaaagt
     1561 aataagttta gatgaacttg atgatgatgc tttggtagtt ccagtatctg gtatgggttc
     1621 tcctgtaatt acaatagaaa aattactttc agaagtagag ttaactaccc ctttagaaat
     1681 aatggagaaa ttattaaata gaaaagtgga tgttataatt cctatagaaa taggagggat
     1741 aaactcactt atgcctatag cagttgctgc taaaaaagga cttcctatct tagatgcaga
     1801 ttctatgggt agagctttcc ctgaagctca aatggtaact ttttatcttg aaggatatga
     1861 ggcatctcct gccgtaatgt ctgatgaaaa aggtaattct gccatccttt atcctgtgga
     1921 tggaatttgg tcagaaagat tagctagaac tttaactgta gaaatgggag gaagttcttc
     1981 tattagtgac tataatttaa gtggagctca agttaaaaaa gctgctatag gtaatactct
     2041 tacaatagct gaaactatag gagggttttt acttgaaaat aaatctgatt caaaaggtgc
     2101 tgttgagaat atactaaaag aattgtgtgg atacaagtta tttgaaggaa aagtaattga
     2161 tattaagaga gagttgaaag gtggatttac tagaggacat gccttttttg ctgggattaa
     2221 tgagtatgat ggagagtatt ctatattatt ccaaaatgag aatttaattg ccaaaaaagg
     2281 tgatacacca ctttgtatta caccagattt aatagcagta ttagatttag aaactggatt
     2341 tccaataact acagagagaa taaaatatgg ctctcgtgtt atggtagtag cttttccttg
     2401 taatgaaaaa tggagaacag aaaaaggaat tgagacagtt ggacctggat actttggata
     2461 tgatgtggaa tataaaacag tagaagaatt acaaggaaaa taaaagttta ggaggaaatt
     2521 ttgatgtatc gtgtaggaat tgacgttgga ggaacaaata cagatgcttg tatactggat
     2581 ggggaattaa aagtaattca ttcagttaag gtggcaacta caaaagatgt tgaaactggt
     2641 gtttacaatg ccttaaaaaa agttatagat gaaagtaaga ttgaccattc attaataaaa
     2701 tatgctatgc ttggtacaac tcattgtaca aatgctattg tagaaagaaa aagacttaat
     2761 aatgtggcta tgataagaat aggaaagccg gccactactg ccattcttcc atttatagat
     2821 tggccagaag acttaagaga aaaaatagaa ttagattcaa tattggtatc tggtggatat
     2881 gagtttgatg gcagaaaaat aaatgaactt tgtaaagatg aagttgtaga gttttgcaat
     2941 agaataaaag gcaaagtgga aagtgttgca ataagtggag tatttgctcc agtaaataaa
     3001 caacaagaag aagaagttgc aaaatgggtt agagaaattt taggggatat tcctatatct
     3061 ttatcaaatg aaataggtaa tataggtata ttagaaagag aaaatggagc tattttaaat
     3121 tctgcattgt ctcaagtagg taaagctgta tctttaggat ttgaaaaagc acttaaagat
     3181 ttaggtatag atgctcaaat atatttcagt caaaatgatg gaacattaat gaatttagag
     3241 tatacaatga aatacccaat ttttacaatt ggatgtggaa taacaaattc tataagagga
     3301 gcttccttct tatcaaaagt aaaaaatgca atagtattag atgttggtgg aactacaagc
     3361 gacttgggtg tactatataa tggattccct agagaatcat ctattccagt tacaattgga
     3421 ggagtacata caaactttag aatgccagat gttttaacta ttggtcttgc tggtggtaca
     3481 tgtgtaaaag gcgaaaaaga taatataaag ataggtcctg aaagtgtggg atatagaata
     3541 actgaagatg caataatatt tggtggagac actctaactt tatcagatgt tgttacaaaa
     3601 ttagatatgg cgtttgaaga tagaaaaagc aatgttgaaa acttagataa agagtattgt
     3661 aatgaggtat ataagaaggt tattttaaaa ttagaagatg ctgtagacca aatgaagact
     3721 agtcaagatg atgttgaatt ggttttaact ggtggaggaa gtataattgt tcctgaaaaa
     3781 ctaaaaggca taagcaatgt tataagacct cctcattata ctgctgcaaa tgctattggt
     3841 gcagctttag gacaaataag tggagatgta gaaaaagtat acagtttaga taaaatgtcg
     3901 agagaagatg ccatagaaga tgcaaaaaaa gaggctgtaa ataaagctct aaaagctggt
     3961 gctaaagaag atactataga agtattaaat atagaagatg ttccacttgc ataccttcct
     4021 ggtaatgcac ttcttataaa agtaaaagtt gttggtgatt taatttaaag aataaatata
     4081 agtatttcca gaattatata aatatttctg gactcatttt taaacttatt ttatcaataa
     4141 aaacagcata acttttgact atattcttat aagtttccga acagccgata tatacctatt
     4201 ttttgaggtt tatcggctgt ttgttatctt ttatcgcatt ccaactattt ttcttaccgt
     4261 atttgctgta cgaattgtaa tcttatcact tacatttgaa ctggcagtct ttgaccacca
     4321 atttgtcttt ttataatctt tacgactaaa agaccaaaag atagcatttt tataatgata
     4381 tactttttca tactcttctt taggatttcc aacctcatca taaaactcgt cataagataa
     4441 gtcaggaaac ataaaaataa gattatcata tatttctttg ttgttatctc cccaccaatt
     4501 aggagcatta tttagtatat cttttaactc ttcttgtgaa ataataaaaa ctggtatttc
     4561 caattcaaac tgttttttta tcattaatct tattgtattt gagataacct tgctgtcatc
     4621 tacatcactt aaaaagacaa cattaccact attaaggtat gtagaaactt ctgaataacc
     4681 aagttctaca aagcttgctt ttaattcaga cataattatc ttgttcttac cacttatatt
     4741 gatacctctt aataatgcga tataactttt catataataa tttcacctct aaacattcac
     4801 atttttctat gtagtacaaa cttgaatttg catcttttat aattctttca aaacttcttc
     4861 atatgtaatt tttttaatat aaaaaaatcg gtgcttaatt tgtaccgatt tttgtttttc
     4921 ctagtaaaga actcgttttt ttgcttctgc tatagctcta gcagcacttt ttgcttgcat
     4981 tcttttttct tcttcttttt taaattcatt tattgtttca tttagattct tatcatgttc
     5041 atttttttct ttatcttcgt tttctctgta tactaatata taatctatta ttgattcgtt
     5101 tattatctga gatatacttt tattttcatt aattcctatt ctcttaactt ccttaagtag
     5161 agtctcatca atatgaaccg ttgtttgctt tgtatttttt gccaccttaa tcacctctta
     5221 aaaaaaattc taacattaaa atttttttta gtcaataaat tttaatatta aaattcttta
     5281 aatgttttat ttgattttct aaaattttct attgtattta tcatcttatt ttttatattt
     5341 tcacctagta cagaagactt tagtctagga ttttcgatat ggtcataaat tggttctaaa
     5401 aattcaactt cgacaggaac agagttaatt ttaccaatag gttggtatcc ttcataagta
     5461 tcttttgaat tttttattac gagtggaaca atagggcatt ttgctttata tgctatcttt
     5521 aaagctccac ttctaaactc cgaaaccaat gaatttggtt ctttaatcca tgttaaatct
     5581 ccttctggaa acactgccat actttgacct gtaagtatat tttgagaggc ttgggctata
     5641 ctctttatac cctcacgatt gttttttctg tttacatata cacatcttag taattttgcc
     5701 cattctttaa atataggtat atttctccaa actggttcat ctgcaattac acatccaatt
     5761 ggtctttcta cacttgctgt tagtatgaag ctgtcaagca tactagaatg atttacaaca
     5821 aatagaagtg gttcttttgg cagtgtttcc ttaccaatta tattaagctc tatattcact
     5881 atatctaaag attttttagc ttgtttttgc ataaattcaa atttttcctt taagctaaat
     5941 ttatcaggat tctttttaat tttaataagc ctaggtatag aagatgtaaa acctataagt
     6001 tgatatgtag caaattttaa
//
LOCUS       DAYASV010000181         5969 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYASV010000181 DAYASV010000000
VERSION     DAYASV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.186"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(315..908)
                     /locus_tag="ACGI68_003491"
     CDS             complement(315..908)
                     /locus_tag="ACGI68_003491"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0139894.1"
                     /translation="MVGAVAGLIVLLYRILLGQAGTWLSQILKFVEGSTIKIIAWFAV
                     LAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKIKQVWWRVLPTKFIGGFLSLMGGLA
                     LGREGPSIQLGAMAGKGISRALDRGKTEEKFLLTCGASAGLSAAFHAPLAGVMFSLEE
                     VHKNFSVSVLISVMTASLTADYISANFLGMESVFQFD"
     gene            complement(1140..1676)
                     /locus_tag="ACGI68_003492"
     CDS             complement(1140..1676)
                     /locus_tag="ACGI68_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFH0139895.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNSVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="ACGI68_003493"
     CDS             complement(1749..2162)
                     /locus_tag="ACGI68_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0139896.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3182
                     /locus_tag="ACGI68_003494"
     CDS             2628..3182
                     /locus_tag="ACGI68_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFH0139897.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQSNINGIEDTI
                     SGMQGTISDMQGSISSMNGTISDMQGSISSMDGTINSMQDSITDMQVEIKEVNSRLDN
                     MEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINRNTLDTVIKHTSSLVKDTSDIK
                     NDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3790..3993)
                     /locus_tag="ACGI68_003495"
     CDS             complement(3790..3993)
                     /locus_tag="ACGI68_003495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0139898.1"
                     /translation="MCLDAVIYMLQTGKQGADKKYLDNMFFVKGYVEELLFLDNSYDI
                     VFSCIVFHHCTNVQNIFCIFWKW"
     gene            complement(4195..4728)
                     /locus_tag="ACGI68_003496"
     CDS             complement(4195..4728)
                     /locus_tag="ACGI68_003496"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFH0139899.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPNFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGIDRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            5045..5176
                     /locus_tag="ACGI68_003497"
     CDS             5045..5176
                     /locus_tag="ACGI68_003497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139900.1"
                     /translation="MGKVRIGCKKEKYFYVRNPVKSLVDRITENEQFAIYHFTHKYN"
     gene            5495..5683
                     /locus_tag="ACGI68_003498"
     CDS             5495..5683
                     /locus_tag="ACGI68_003498"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0139901.1"
                     /translation="MPYKYFTEFCLLKATQFLKGTNELISDIAIKVVFHQIIYFSKCF
                     KEKTGYSPKVYRNIKKEI"
BASE COUNT         2140 a         1000 c          739 g         2090 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact tagggtttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 ttcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgctagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc acgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actattcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tacttactaa atataagaaa ataattgctc aatttctcaa taaatgtgaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattaa ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ctaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa cactattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatagcag tcttatccca tatacttttt aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctatct tgataaatag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttgcacttac ggcagtgttc tggctttcct
     2041 gtatcgccgt tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aaatattcaa
     2341 acacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gtagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa gcaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta atattagtaa tatgcaaggt agtatcagtg
     2701 gtatcgaagg cactattagt gatatgcaaa gcaatattaa tggtatcgaa gacactatta
     2761 gtggcatgca aggcactatt agcgatatgc aaggcagtat cagtagtatg aatggcacta
     2821 ttagcgatat gcaaggcagt atcagtagta tggatggcac tattaatagt atgcaagaca
     2881 gtattactga tatgcaagtt gaaattaaag aagtcaatag taggttagac aatatggaac
     2941 ctgatattaa agacttaaaa actggacaag ttgaaactag tagtagactt gatactattc
     3001 agagtgatgt tacagaaatt aagtcagatg taaaaattaa cagaaataca ttagatacag
     3061 tgattaaaca tacctcttca ttagtaaaag atacatcaga tattaaaaat gatttaaata
     3121 atatagagat agtcacatct aagaattcga atgatattgc taaattaaaa tcagttaaat
     3181 aatatactat agtaaaaaat acctgaaaat ttcacatact tctttaaagt tataccaaat
     3241 tttatacaga aatactgatt atccgacaac acactttact tataaataat ttattaaaaa
     3301 aatactcatt ttatcctaaa attaacacga ctaaatgagt cttgatataa taataaaaaa
     3361 taagggagat tgaacactaa aaagttgtgt atctccctta tattctataa tatagttata
     3421 taatttttca gcctaaaata aaagtccatc tttgattaaa acacatcccc ttatcactta
     3481 taaaaggata aaatcttgtt ttcatttttc catttatatc ttcttgctta agagtaataa
     3541 ttttttcttt tgatacttta agtgattggt taaatttaac caaacgacta agttttgttc
     3601 aacatctatt tttcatattt tccaatactt aatctatttt ttataaataa ttcttttatt
     3661 tctaactcac ttacattttt tacataaaac gaagcatata aatttcaacc ttatcttgta
     3721 tggttcttaa tacttaatga gtagcttaag tatcaatcat tataaatttt tcccttacat
     3781 ttaacactct taccatttcc aaaatataca aaatatattt tgaacatttg tacaatgatg
     3841 aaatacaata catgaaaata caatatcata actgttgtct aaaaatagaa gttcttcaac
     3901 ataacctttc acaaaaaaca tattatctaa gtattttttg tctgctcctt gttttcctgt
     3961 ttgtagcata taaattactg catctaaaca tataactact cacacgaaaa aagaaaataa
     4021 acttccaaaa tacaagctct aaaaactacc tctaaagttg tataatgttt actaggaata
     4081 aggttgtata acatatagtt taaataatca ttttttaaaa ttgcaagtta ttgctttcaa
     4141 aattagatgt ttttatatca aataaatgat taagaatttc taaatttaat ggctttataa
     4201 tgcactccct ttctttggga taaaataatg ttgcacagat actttttcta aagataacaa
     4261 ttttattttt ctatcaattc caccagcata cccagtcaaa ctcccatttg ttcctacaac
     4321 acgatgacat ggaataataa tagagattcc attatgccct actgcaccac cgattgcttg
     4381 tgctgacatt ttagatattc ctttttgttg agcaacttta tatgcaattt ctccataagt
     4441 gattgtttta ccatatggta tttgttgtaa aattttccac actgtagtct gaaaaggagt
     4501 acctaccata tggagtgatg gtgtaaaatt gggttctttt cccgaaaaat aaatatctaa
     4561 ccatttcttt gtctgctcga aaattggcaa ttctttttct tgatgttctt ggtcaagatt
     4621 ataagcataa tacttttgat tctcaaacca aagaccagtt aacccaatat tatctgatgc
     4681 taaaagaata tttccaattg gactatggta atagctaata tattgcatac aattcctcct
     4741 tatattctga aaacttcact caaatcatta gacacttctt ttctatctac cattatgtaa
     4801 aaacatacta atataatttc cttctaacta cactttcgtt ataatatcta tcctcatatc
     4861 atattagttt tatatttacc tgcatctgtt catccttttt tccctttccc tttgatgaat
     4921 ataaaattca tacatttaag cttcctactg tattaacttt tatagaagtg tatattataa
     4981 ttttatcact aaataggcca aaaaaatatc gatatagcat caagtaatat cataatactt
     5041 tcaaatggga aaagtaagaa taggctgtaa aaaagaaaag tatttttatg ttagaaatcc
     5101 agtaaaatca ttagtagata gaataacaga aaacgaacaa tttgctattt atcattttac
     5161 acataaatat aattgacgca ataaagtgtt ttctattcta caacgattaa taaggttaga
     5221 aaaaagtaaa aatgaactgt atatgtatga agcattagtt tctgtttctt tcctttggct
     5281 tattatgata aaaaatattg ttttgctacc aataaaacac aaaaatataa ttaatatcca
     5341 tatgcaaaaa attttacgtt atattgaaaa atactattgt tttacattat attgaagggt
     5401 actattgtga aaacttaatt ttggagtact atcaagcagt ggtaatatca ataaatcaga
     5461 gtgctccaga tgatttgaac taagtttaaa tactatacct tataaatatt ttactgagtt
     5521 ttgtttatta aaagcaactc aatttctaaa aggcacaaat gagctaataa gtgacattgc
     5581 tataaaagtt gtttttcatc aaataatcta ttttagtaaa tgttttaaag aaaaaacggg
     5641 atattctcca aaagtgtaca gaaatataaa aaaagagata tgatttttta tttcttttga
     5701 ttatccaaag agtaaataaa aaatattgta aaaaacttac taacaacggc aatgctttga
     5761 actatggtaa tactttgaac ttttgattgg actttttttg ctaaaataat atatttaaat
     5821 agcaaagtaa tctgtttaat caataaaaaa taagagataa aagaaatcaa ctaaaacttt
     5881 tacctcttat tttgatagtt tatcagagct aatatttaca ctctttttat ttttgatact
     5941 tcattaaaaa tgatgcttac tctccattc
//
LOCUS       DAYASV010000182         5636 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYASV010000182 DAYASV010000000
VERSION     DAYASV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.187"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            197..1378
                     /locus_tag="ACGI68_003499"
     CDS             197..1378
                     /locus_tag="ACGI68_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0139902.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1520..2437)
                     /locus_tag="ACGI68_003500"
     CDS             complement(1520..2437)
                     /locus_tag="ACGI68_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotA family spore coat protein"
                     /protein_id="HFH0139903.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2690..3484
                     /locus_tag="ACGI68_003501"
     CDS             2690..3484
                     /locus_tag="ACGI68_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139904.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3485..5545
                     /locus_tag="ACGI68_003502"
     CDS             3485..5545
                     /locus_tag="ACGI68_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HFH0139905.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLTVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPAICEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2158 a          633 c          921 g         1924 t
ORIGIN      
        1 tattttaaga aagacattta tttataatta tatacaaaaa tattttttaa tgtttttgtg
       61 tataattata acaatttaat ttatattaat gcacttaaat ttgaaataga atgcttatag
      121 tggtaaaatt aattattaga atattagaaa taataaataa ataatacatt gtacttaaaa
      181 gggggagaaa tttaagatga acaaaaaata tattaaagca gggaatttaa ttgatggaac
      241 cggaaatagt attatgcaaa ataagggtat aattattgat ggagatacta tagttgaaat
      301 tgaagatata agagatgatt taaatggata tgatgtgtac gattacttag ataaaactgt
      361 tatgccaggc attatgaatt gtcatgttca cttgacaatg gagccagttg gaaatccaaa
      421 gatatattat gataatgtat cagatgtaga gcttgtagta aatacgataa aacaacttga
      481 tgcttatttg gattcaggag taacatatat tagaagctta ggttgtccca aatatgtgga
      541 tgtacaactt aaaaatctta tagacaaagg gtctatagat ggtcctggta tagttacttc
      601 aggtccagtt atatgtatga caggagggca tggatattat tttggtattg aaagtgatgg
      661 agtagatgaa tgtagaaaat cagccagaac agttttaaaa aatggagtag attgtgtaaa
      721 gataatggca actggaggtg taacaactga tggagttgag ccagggtcac cacaattaac
      781 tttggaggaa atgaaggctg ctgtagatga agctataaaa gcaggtaaaa taactgccac
      841 acatgcacaa ggaagaactg gtataaaaaa tgcagtgctt gcaggaatta cttctataga
      901 acatggtgtt tatttagatg atgaaataat tgatttgatg cttgaaagag gaacttactt
      961 agttccaact gtggctgcac catattttat tctaaatgca ggaaaagaaa gtggaataac
     1021 tgaagctact cttagaaagt gtgagattgt tgccattcca cacaaagagt cattcctaaa
     1081 agcatataaa aaaggaatta aaattgcagt tggttctgat gcaggaactt cttataatga
     1141 acatggaaaa acatattatg aaatgaaact aatgtcagat tatggtatgg ataatatgga
     1201 tataatagtt gctgctacaa aaacagcatc tgaactttta agaattgata aaaattatgg
     1261 aactctagaa aaaggaaaga aagcagatgt tatagttgta aatggaaatc cagtagagaa
     1321 tatagacgta cttgcagatg tgttagcagt atttaaactt ggtaaaaaag ttagataaat
     1381 agtataaaaa taaaaaagag tgtctaaaga aatttttaaa taattataga cactcttttt
     1441 tattagtaat aattaaatct tttatacttt agaaaataga ttctatttta tatttagata
     1501 taactcttta aaatcattct tatgcaatat aatctataga atctacacat acaaagtata
     1561 ttcttgaatc attagaattt ccaagtacta taacatcatt tttcttacct agaacttcaa
     1621 caccagttag aaccaaacta ccagctacta cagtaacttg attatttata tttgacatat
     1681 aaagattatc taggatacct ttattacaac agcaatcatc acaatcgcag ttgcaattac
     1741 attctttgta atgtttttta tcaagatagt aacctaaaga tgctatgaaa tcttcataat
     1801 ctgcaggtgt aatatcaaat gcaacagctt taagattaca caatgatgct aaatctacgt
     1861 ttgctaatgg agtagatgca gcaagtataa aatcaattat agcagcaagt attgcatcta
     1921 atatagcccc aaggtctatc gtaggaggaa taactgcatc aataagtgca attaattcag
     1981 ctactcctgg tatagttcct atagtattaa ttaatccctc aagagttaaa gggactggaa
     2041 taggataaga tactctacca gctatatcta ttaaatcaca gttacaagca gaaaatcttt
     2101 caaaagtacc atcaagtcca gataaattat cttttggagg agctgaaaga tctataccta
     2161 ccaaattagc acctactata aagaaatctg agataaaagc aaattgatta aagtttacaa
     2221 aaggtcttag agcatcatat cttagaagtt ctaatgcttc tctcatactt tttttacaac
     2281 aacattgttt tattttaggt tctttatatt tatttggata attatagtat ctatcagcta
     2341 cttgataatt ttcatagtat ctttcatttg tatttggata atcttcctca tattcttgtg
     2401 taaatctaaa gtcctctcta catttattat tttccactaa atacactcct tttatatttt
     2461 aatttaaatg tatagataat taagcttatc tatatagtaa tattatattt ataatgtaaa
     2521 aaaatgttac aaaagatatt ataaacgaat aaaaaataat aatttgtcag ataaacttgt
     2581 aaatttttat gaataatcca ttctaaaaca aaaaactgtg tataaaaaag gtttatatgt
     2641 tatatttata taaatataac atatgaacga gaaaggagtg gtttgataaa tgattgaggt
     2701 agataaattg tgtaagtctt ttacaagggt tgtaaaagat gataagaata aaagttcaat
     2761 aagaaagcta aagaaagtaa aaactaaaaa agaagaattt tttgctgtaa ataatgtttc
     2821 atttaaagtt atggaaggtg aaattgttgg aatattaggt ccaaatggtg caggaaagac
     2881 tacattactt agaatgttag gtggcatatt aacaccaaca tcaggaagta taaatatttc
     2941 tggatatgat tattcaatag atagaaattc agcaaaaaaa gaaataggtt atctctctgg
     3001 aaatacaaaa ctatatggaa ggctttctcc gagagaactt ctaacaactt ttgcaagtct
     3061 atatgagatg tcaaaagaag atatagaaga atcaattgaa aatattgtaa aaataatgga
     3121 tatgagtgaa tttatagata atagaattga aaatctatca actggtcaga ctcaaagaac
     3181 atctatagct cgttgtttaa tacattctcc aaaagtatat atttttgatg agccaacact
     3241 aggtctggat gttttaagta gtgcatctat tatagatttt atgaagaatg aaaaactaag
     3301 gggaaaaact gttctttact caactcatta tatggaagaa gcagaaactt tatgtgacaa
     3361 gatttttatg attcacaatg ggaaaataat tgcatcagga acaccacaga gtttaaaaga
     3421 ggaatctgga gtaaataatt tgagagatgt attcataaaa cttgccagaa aggaggacat
     3481 ttaaatgaga agtaagattg taaaatacat atttaaaaaa gaaatgttag acatattgag
     3541 agataaaaag actttattta tgatgattgt tgtgccactt cttttatatc ctttacttat
     3601 gttattaatg attcaagtaa tgaatatgtc tgcaagtagt atgaatagta aggacattaa
     3661 tatagctttt aataataaac ctaataaggt gttagtatcc atgattgaag atgataattt
     3721 agaaaaagat actaaaaata cttcaaatga taaaggcaaa ataagagtgt tagatgtaga
     3781 taactataaa aagtcattag aagaaaataa aattgatgct tatataaaga tggaagaaaa
     3841 aaaatcatct ataaattatc aaatatatat aaattcttct caagaaaatt cttctaatgc
     3901 actaaaaaga attgaaactg ttttggataa atataaaaga tttaccatag aaaaaacttt
     3961 atctgaaaaa ggattagatg tacaagctac tttagaacca ataacatata gcactgtaga
     4021 tgttgcaaag acggaagaag tggcaggata ctttttaggt cagatacttc catttatatt
     4081 aataatagga gttttactag gttcaatata tccagcaata gatgttatgg ctggagaaaa
     4141 ggaaagaggt acattagaaa cattatttac actgccaata tcaaacttag aactggttat
     4201 gggaaaatat atggcagttt ctcttagtgc aatagtaaca gcaattttaa acattttatc
     4261 tatgggactg actatggcat ttattttagt tagtggaggt attagtagcc aatttggctt
     4321 tggaaacttt aattatggag acttagtctt accaattata actactttaa tttgcatatg
     4381 tcttttttca atggtagttt cagcaataag tatgtgtgta tgttcattag caaaaagttt
     4441 taaagatgct caaaactaca taacacctct tatgttaatt attatgatac catcatatgt
     4501 ttctatgata ccaaatatag tattagatag agtgacagct gtaatacctg tagtaaatat
     4561 atctttactt ataaaaagtg tattatcttt taaaagcgat ttaaatttaa tagccttagt
     4621 tttagcatca aatgtagcct ttgttatatt ggcaatcata ctactgtcta agatgttcaa
     4681 ttctgaggaa atactttttg gtaatagtaa gagtttttct ttcttagaga aaagaagtaa
     4741 tataaaaaaa gggtctatac caactgtaag tgatggtgtt attttatatg cagtaggatt
     4801 agttctattg atttatgttg gttctttagt tcaaattaaa tttggtatgg caggtattat
     4861 aatgacacaa ttcatgataa tttctttacc attattattt gcatactata ttaaagctga
     4921 ttttaaagag gtatttagct tgacagttcc taaattaaag catgtgtttg ggagcattgt
     4981 tctttggatt ggtggataca tacttattat gattataaca cagctaattt tattcttatt
     5041 tcctcaaaat atggaagttg ctgaaagttt aaataaggct ctttttatca aagatagtgt
     5101 tttactgaat ttactagcaa tagctttatt accagctata tgtgaagaaa tgttttttag
     5161 gggatttata ttttcttcat ttagtaaatc aaaagataaa aataagtcta tcaaactcgc
     5221 tataatatgt agtggggtac tatttggtat tatgcatatg gattttatta gaataattcc
     5281 aacatcaata ttaggtatta tatttgcata cagtgtgtat aaatctggtt caatatttgt
     5341 atcaatgtta ttgcattttt taaataatag tgtagcagtt gtgttaaatc attatacaac
     5401 tggaaatata accaacttat ataaatttgt tgaagttgat ttttcgaact taaatgtgct
     5461 taaatttgcc ttaattcttc taataagtct tatattaatt atgttaggat taagagtttt
     5521 agatagaaag tctttaagaa attaagtata aagttattat atagattaaa atattaataa
     5581 gcactaaagt atatggatat atcttgtata taaaaatact ttagtgctat tttata
//
LOCUS       DAYASV010000183         5609 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYASV010000183 DAYASV010000000
VERSION     DAYASV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.188"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            473..952
                     /locus_tag="ACGI68_003503"
     CDS             473..952
                     /locus_tag="ACGI68_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFH0139906.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            949..1728
                     /locus_tag="ACGI68_003504"
     CDS             949..1728
                     /locus_tag="ACGI68_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139907.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFIPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1978..2478)
                     /locus_tag="ACGI68_003505"
     CDS             complement(1978..2478)
                     /locus_tag="ACGI68_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFH0139908.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(3090..3797)
                     /locus_tag="ACGI68_003506"
     CDS             complement(3090..3797)
                     /locus_tag="ACGI68_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFH0139909.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(4600..5469)
                     /locus_tag="ACGI68_003507"
     CDS             complement(4600..5469)
                     /locus_tag="ACGI68_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFH0139910.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         2082 a          830 c          570 g         2127 t
ORIGIN      
        1 atttatgttc atctctatct aaaataatga catttctaac tcttaattct catttaagtt
       61 tcattgtttt ttcttaaaag cctactttta gtcaagatac tgttatcttt actcattatt
      121 taaagaactt attttaaatc tgtacatgaa gtaaataatc caaaagcatt gttattctaa
      181 ctactcaaca ttaaatactt actattttct aattactcaa tataaagact tactataaaa
      241 ctaaatacat aaatggcttc taaagtttaa tatatctaca tggctttata tatgacagga
      301 aaaatactag aaaaaatata ttatagaata cttgtatata ataggcaaaa ctttcaaact
      361 taaatttatc aaataatcat aaaaaaagtg aaccatctat agtaaaattt tatctaataa
      421 gtgaaaggca aaaaagtagc gctacgattt gcatagaagg gaaggaactt agttggataa
      481 aactacattt acaaacaata tacttgaatc agaacagact ttgtatcgag tatctaagtc
      541 tattctagga aatgaccaag actgtgaaga tgccgtcaac aatgctattt taaaagcata
      601 tgaaaagttg gattctctta aggaagaaca atactttaaa acatggctga ttagaatagt
      661 cataaatgaa tgtaattcat taagacgcaa acgattaaaa tctctatcat ttgaagatgt
      721 cttcaaaaac aaaaaaattg atgaaaaaga tgactatagc gatttgtata ctgctattca
      781 aagtctacct aaaaaaatta aaattccaat agtcctttac tatattgaag gttattcagt
      841 agatgaagtt aaggaaatat tagatatacc tcaaggaact gtaaaaagta gattatcaag
      901 aggaagaaga ttattaaaaa ctaaattaga aaacacagag gtaatcatat gaataaaaat
      961 atattaaata attcgtttcc agaagttcca gaaagttttc ataatagttt aagcaaaaca
     1021 ctaaatagtt taccagaaag agaggaaaat tataaaatga ttaataataa aacacacaaa
     1081 ttaccattta gaaaaggctt agttgcaact ttagccatta ctttgatatt gagcacaaca
     1141 gcccttgcag taggacatat atatagttta gttggaaaat caagtactaa ggctacctat
     1201 acatctatcc caactacaga acaagtaaaa aaagacttta actttacccc taatatagtt
     1261 tctgaattta gtaatggata taaatttaaa ggagcatata ctgtaaataa aaaagcattt
     1321 gacaaagaaa ataatgtttt aggaaaatca aaagcactta gttttgaata taaaaatggc
     1381 aatgatgaaa tatctcttga cacagcaaat aatgtacttg ggaagagttc tgacaatcta
     1441 aaagttgtag atacttacaa agatattaat atttcttatg aagcattcgc acaaaagttt
     1501 ataccagaag gttataaaat gacagaacag gataaaattg atgaaaaatc aggcaaatac
     1561 acatttagtg tggggccaaa taatgaatca gacaaagttg aagttaatga atttaagttt
     1621 ttgacatgga aacaagatgg agtttactat tcttttacag ttcaagactc aaatttatct
     1681 atgaatgact tagttaaaat ggctcatgaa gtaatagatg caaaataaac ataagtaata
     1741 ataagttgtt tatgagttta ttatttatga ctttaatttt tttattagta aacatggttg
     1801 tatcttccat ctttatattt tctttaaatt taatatataa aattttagat atttagctac
     1861 cttcaaaaga agcattatta aaaagccctt aaaaatattt cttttatatg gaattttaag
     1921 ggctttaact ttattttata caagtgtata tggaatttta aagactttaa ctttatttta
     1981 tataagtgta agctccattt gaattacttc ttcttttaca ggtctatcat tccatgtaaa
     2041 accactagat attgtattta cctctacaaa ccctaaagat ttataaacat gaatagctct
     2101 actattactt ttaaatacat caagctcaat tttttcatac ccatattctt ttgataaatc
     2161 aataatttgt tttaaaagac tacttccgat acctttttcc ctatactctt taagaatacc
     2221 catgcctaaa taaccaactt tttcagtttt aggtaaaacg tcacaccaac caatacagtt
     2281 atctgattct aaatcaatta taaacagttg tggtaagttt ttatttataa tattttttat
     2341 aaattcaact gtttcttcaa aaggaaaggc ttcatccata gctagatatt tcctctcttt
     2401 tgctatttta tcaaaagttt gccaatacga ataaatatac ttttcttctg cctttataaa
     2461 ttttatgcct tctatcaact taacatttcc cctttttaat ttttgattgt agttaactta
     2521 ttttatatga ttctagttta taatgctata taaattacta tatattagct taaaatatct
     2581 ttgtatattt ctataaaatt aaaaccaata tatctatctc ttgcatctct attaaaataa
     2641 tttttaatag tataataaat atagttcttt aatcttttgg gtatatatac ttaatcattt
     2701 tgaaatttga tttaaagtat aataaaatat ataaaaaaat atataaaatg gtactataaa
     2761 atttttaatc ttcatcaata ccttttttgc tttcattgtt aaaactattt tttctattag
     2821 tttatttctt ataaaacaaa catttagtat taaaatatat atcacacaat agtatttgca
     2881 atttaacttt aaaaaaataa aaatacccaa ctgacctaag caactcgatt gggtataaat
     2941 aatctaaaaa atcatttttg tataaactct ttatttaact tacattttta tttaccttct
     3001 actttaattc aacttatatt tctatgcttc ttatactctc attcaactta tatttctact
     3061 tatcttatac ttttataaat tcatcacaat tatttagttc tcgctatcaa agaaattgta
     3121 ttaaaatact ttccattctc tacttccatc atttttttgt catttatagc atatgaatta
     3181 tcacaattta tccaatcctt ccaagcttca tcatgacaat ttaaagcttc acatttctct
     3241 acaatcacag actccgattc agaccaaagt ttattccacc atgtaattga atggaagttc
     3301 atattatctt gccaaaatgg aatgagttca tctggtatac aatccacgaa atcttctttt
     3361 aatccaggca ttgccatagc aagtattccc ccctctttta caagaggtga tatatgattg
     3421 tctaaaaatc cttctttgtt tccaaaatag tgataagaat cgatacttat caccgcatca
     3481 aagaaacctt ctgcataagg tagttcatgt gcctctgcct gtattggaaa tattttatca
     3541 tccaatttaa actctttaaa tcttttatag ttttcagttg gttctatcca taaatctgtt
     3601 gcaaatacag tagcatcaaa ttcctttgct aaaaatatcg aagatatacc cttaccacaa
     3661 cctaagtcta aaatacgcat tcccttttct agttttatct tacttgttaa ttcttccaat
     3721 atttttatac aattaggtcc catcatattt ttcatgagaa agtccttatc gtatttatta
     3781 ctttttgtaa atatcattct atctttcctt tccctatcag ttattattta ttaaatttta
     3841 tacgtttgat tttttatttt ttatctgctt cttatattga gcaatctctt ttttattcat
     3901 tctttaacag acaattttaa tgtgtaattt tatccactct gtgatagtaa ctttaatgaa
     3961 taatcttatc tactgtgata gttattttag tgagtaattt tatctactcc acagcaatcg
     4021 ctttaatgag taatcttatc cactctatga tagttatttt agtgagtaat tttatctact
     4081 ccacagtaat cgctttaacg agtaatctta tctactctat aatagtcact ttaatgagta
     4141 atcataattt ttatatcatt ttagatactt actctaataa gaattcattt tctaatactt
     4201 aataagtttt aattacttcc actttaacct atatactaat taacttattt aatttagtgc
     4261 atatttgaca ctttaccttt tattatacca tttattatca taaatataat aattctattt
     4321 ttttaaaata aatatttata ataatctaaa ataaatattt ataaattcta atcaaccgta
     4381 aataataatc taaaatacat caaaaaataa actaaaaaat atcgaaaaat aatttaaaag
     4441 acatcaaaac taaatcttct aatcagcaat taaaagtcta ttttcttagt ttaaaaaata
     4501 aagatagatt ttttcaaaac actggataaa taggtaaaaa aataaacagt tagtaaaata
     4561 aatttttact aactgtttat ttttattgtt taatatgtcc tataaatcct ctaagtaata
     4621 ttttccttgc aaaaattctg attctaaacc tagattttta aaataatcaa ccatgcccat
     4681 acacgcaaat ttttctgtag aataatgagt agcacctata acattaatta aattatcttt
     4741 tgctatttga tggaactcta atactggttc aaaatgtttt aaaggcttag taaacccagt
     4801 aatatataga tttataccaa gttcagcaac ttctcttgct acaaatggat aacttcctcc
     4861 ccctgctgca atagcaacta aaccatcttt taatacagaa tctccataat catacaattt
     4921 cacatcatga ccaacagatt ttttaattaa ttccttaatc tcttctattg attttagtga
     4981 tgttttacat ataactccta cctcaatttt atcgtcatat ttacaaaatg gtttgactat
     5041 ttctaaaccc atagcttttg caaaactaac agaagttgaa tagtcactat agttgtctag
     5101 tggagaatgt aatacataaa aagaaattct acgatttttc atctcttcca tataagatag
     5161 tggtatgtcg tgaaatggaa aaccttcatc tgatgctatg tatcccattg catgatgtga
     5221 aaaaattaaa atatcttttt catctttatc tataacttgc ttaattattt ctttatcagg
     5281 aaaagtagtt gtgtatactt tattaatatt ttgagcatta tctaatacta atcctatgta
     5341 tttttttcta aattctggag taataaaatc attttcaaaa ttcataaaac tccaatcatc
     5401 attaatattt ttaatattaa attctaaatc taatttttta taaagttcaa ttgccttaat
     5461 aataaccact cctttataat aatattgaat tactacatat taatatagaa atttccgata
     5521 ataaacttta tacgtttaag tattcctact tcaaattaaa aatattataa atattcaaat
     5581 ttattaatat attttaatat tatatttat
//
LOCUS       DAYASV010000184         5590 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYASV010000184 DAYASV010000000
VERSION     DAYASV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.189"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            121..621
                     /locus_tag="ACGI68_003508"
     CDS             121..621
                     /locus_tag="ACGI68_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFH0139911.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            678..1664
                     /locus_tag="ACGI68_003509"
     CDS             678..1664
                     /locus_tag="ACGI68_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0139912.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1714..2436
                     /locus_tag="ACGI68_003510"
     CDS             1714..2436
                     /locus_tag="ACGI68_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HFH0139913.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2465..4399
                     /locus_tag="ACGI68_003511"
     CDS             2465..4399
                     /locus_tag="ACGI68_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0139914.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4392..>5590
                     /locus_tag="ACGI68_003512"
     CDS             4392..>5590
                     /locus_tag="ACGI68_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFH0139915.1"
                     /translation="MSKNIYVKETYEWIRIGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLLLLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKT"
BASE COUNT         2336 a          428 c          960 g         1866 t
ORIGIN      
        1 aaaatagttc gctaaaataa tttgttttta taaaattact gtttaagttt atagattttg
       61 tataaaataa agttatgtta ttaatttttt taaaaataaa acttttacta ggagacagat
      121 atgagaaaaa atagtattaa tatagcacta actgcagcaa ttacagcaat ctatgcagtt
      181 ttaacattat cattgggatt tataagttat ggaccaatac aatttagggt agcagagatt
      241 atgatgcttt tggcattttt agataaagga tatatagtag gtcttacatt aggttgtttt
      301 ttagcaaatg taataggacc atatggtgta cctgatataa tttttggaac attagccaca
      361 tttataagtg caagtatgat ttatattact agaaagttag gtaaacaaaa taaaagtact
      421 ttagttatag cttctttatg gccaactgtt ataaattcga ttgtggttgg tctaatgtta
      481 aatgtattct ttgggcttcc attaattcta tctatgatac aggtaggttt tggagaattt
      541 gtagtagtaa caattgtagg agtaccattt ttctgctttt taatgaaaaa atataaaaat
      601 gtcattaata ttaaatttta aatagtgtat agtaatttaa ttaattgaga gtattattag
      661 tgaaaagagg tgtttatttg tcgaaggtaa aaagattgac tgatgtttgg atatatatta
      721 atgataagca aaagtttaca ataaaagagt tgtcagagga atttaatctt tcagctaaaa
      781 caatacaacg atatttaaaa gaattaaata aaatgggact tccaattcaa gcagaacaag
      841 gtagaaatgg aggttatagg gtgctaaata atagctatat acctcctgtt atatttacag
      901 aaaaagaagt tatgattata ctatttgcac ttaagtcact tcagatttat aattataagt
      961 taatggaaat agaaattaat tctataatga gaaaagtaag ttatgaacgt aaaagcagta
     1021 taaaagtagg tatagaaaat attaaaagat acattggctt tgtagctaat gatactaatg
     1081 atggagattt taagagtagt aatgtaactg agttttttag agcttcttca gaatctctaa
     1141 ttttaaatat taagtataag tgtggtaatc agattttgga aaaaaacgta tgccctatag
     1201 gtatatattt aaataaggga gcatggtttt caccagcata tgattgtaaa agtgataaaa
     1261 taatattagt tcatatagat aatataattg atataaaaag aacaggagaa agtaaaaaaa
     1321 tacatataaa cttggaagaa tggttacatg aagtatataa caatttatat ggaaatagtt
     1381 caactactaa aaaatttggt gaaattattt atctaaatgt attattaact aaagaaggtg
     1441 tttctaaaat aaaggacagt tcttggaatt tagatatgga gctaaatgaa gatggtagtg
     1501 gaataattag tacattcata aaggttgaag atatagattg ttatgcttcg attttatttt
     1561 ttattggaaa taatgctaag gttatagagc caaaattttt gaataagcta ctttatgata
     1621 aggccaatga gttaagatat ttttatgaag aaaatatggt ataatattta gaaatgttga
     1681 aattaaataa ttattaagga gatatacgtt ataatggaaa attttaataa gaatgctaaa
     1741 ataattggta ttggagctga aggtattaat ataataaatg aagtagaaga aaaaataaaa
     1801 gcaaatatgg atatagaaaa aataaacata aatcaagaga ttgaaaaaga atatgttaga
     1861 agtcttttag atggtgtaga tatattattt ttgatatatt catctgaaga taaacatatt
     1921 agagatatta taaaagctgt ttcatatatg tcaaacgaaa gaagagttct atctatagga
     1981 atggattgtt cagagaagga aaataaagaa gatttagagc ttgggagaga gtttaaaatc
     2041 aataatgata gtatatttaa atttgttgat ttaatgaata tcatggtaga gtctatttct
     2101 gattcttgca tgataaatat agatattact gatttaaaag aagctattgt aggagataaa
     2161 ggaattaagt attcttttga agagtttgaa gatacaaagt catatagcga gatagctgac
     2221 attttatttg acaggatgga atatattggt gaggaattta taagtaagaa aggaattgta
     2281 tttgttgaag gaagtccaga attttctata atggaattga atgatttaat aagtaatatt
     2341 cagagcaagg tagaagaaag ctatgaagta atattttctt tatatataaa ggaaaattta
     2401 aatggaaata ttagagtagg cttattgtat aattagtata ttttagatat taaggagagt
     2461 gtatatgtct gaaaatataa gaggtacaga gtattggtta atggaagaat ttttttatca
     2521 ggaaacagac acaccacaga tttttagaga agaaagcata attgcacttg gatttgatat
     2581 aaaaataaat ttaaaaaaag tattcagtat ggggaaaaaa tcagaaaagt atattaatga
     2641 gaattgtaat gataaagatt ttataaaaaa gtttatgtcg attaaaaaag gagattattt
     2701 tttatataaa gcatatcaaa atatagaaga aaaagttaaa tgtaatgtgt ctattggcat
     2761 agttgaagaa gattttgaaa atggatatga attaagtcat attttagggc atacactacc
     2821 agtaaaatgg atgcataatt ttgatacagt ttcaaattgt aaagtatata caagtgaatt
     2881 ttataaatta aataataatg atattagttc atatttagaa attattaagt cacaagataa
     2941 gaaattattg agaaaaagaa tagaagaaag tgaaaataat aaatggtatt taaaaaacta
     3001 ttattataaa aatgtagata agtgtgtaga tgatgaagaa ttatttattc agcccattaa
     3061 tctagaacaa ttagaaagcc taaaaaatat aaagacagat gattatattt tattgtataa
     3121 tagcgagaaa gtaatttgtg aaaatgacca tacaatgaag agtctatttg gcgtatgtca
     3181 agttatggaa gggttcgata aagagaaagt aagtgctttg gagggtggat atttttttcc
     3241 tgttagatgg atagagtatg aaaaggagaa tatagacgga aatgcatggg ttatatttga
     3301 aaataatagt atagttgaaa agtatgtaaa aagatttact aataaaatgc cattaaatac
     3361 tatactttat ggaccaccag gaactggtaa aacatacaat acaaaacaga tggtttacaa
     3421 tctagtaagt gaaaccagtc taagaaacag atactataca ggttacttag attataaaaa
     3481 taatgtagaa ttttgtacat ttcatcaatc atatggatat gaggaattta tagagggcct
     3541 taagtctgat ggtgaaggca attttaagcc agaagatggg atattaaagg atatatctat
     3601 agaagcatgt tataatgcat tgaaatttga aaaaaagatg agctttgagt tggaaaaaga
     3661 tagcttgact ttagaaacta taaaagacag gaaaaagaaa ttagtattag aaaatataga
     3721 atatagtgag agttttaatt ttgaagaatc agaaaactat gttttaataa tagatgaaat
     3781 taatagaggg aatgtatcta aaatatttgg tgagctaata acattattgg aagatgataa
     3841 aagacttact aaatcaaatc aaactattgt caaactacca tattcaaagg aaaaattttc
     3901 tctacctcca aatttgtata tagttggaac tatgaacaca tcagataaat caatagcact
     3961 tttagatata gctttaagaa gaaggtttaa ttttgaagaa attatgccag attatgattt
     4021 attgtctatt gtagatggta tagatattag aaaaatgcta tatacaatca ataaaaggat
     4081 agagttttta tatgatagag accatgttat tggacaagct tatctacttg ctgtagaaaa
     4141 tattaatgat atagtagtag tatttagaag taaaataata ccacttcttc aagagtattt
     4201 ttattatgat tcagaaaaag taggtttgat tttaggagga attggaagaa atgaaaaaga
     4261 taaatacatt gtttataaag aaaaacttat agcttctgag ttatttaaaa ataataatgc
     4321 ctcaataatg gagagtaaag ttaactattg tataaaacaa aacatatcta gtgaggagct
     4381 aaaaaatata tatgagtaaa aatatatatg ttaaggaaac ctatgaatgg ataaggatag
     4441 gtaatggtga aaatgagctt acagaaattg aatatgaaaa attgcttaag tatctagaga
     4501 acaataatga tgtattaaaa agtaatataa tagatattaa atacaaaaaa cttagattta
     4561 taaactatgt aggcatcata tgttttgaaa atgtaatact tgaaatactt cctaaattgt
     4621 cattaagtga taatcttgta aaagatagag agatactatt acagatgctt tctatatgta
     4681 ataaaattcc aattacaatg aatgaaaaaa taagattgag tttgaaaaat tataatctct
     4741 taaatttttt tgttatgtat ttcattgaga gtatgcaaac acagatgaaa aaaggtattt
     4801 attttgaata tataaataaa atagagaact taaatgttat gaggggtaaa atattattaa
     4861 gtacatatgc aaaagaaaaa ggcatttctc caatgaaaat aagatgtgaa tatgatgagt
     4921 atagtgaaaa taatttcttg aatcaagttc ttaaaaaagc atgtatatct attttatgta
     4981 gaataaatga caattcaatt caaggcaaga ttaaaaaaat tttaagctat tttcaaaatg
     5041 tagatttaat ttatatagat agaaaaaaac tgttggatta taaattttat aaaaacaatg
     5101 atagatttaa agattgttat ttactagcta gacttctact tttaaatttg tctatggata
     5161 atagtcaaaa taatcaagag gcattttcta tattatttga aataaatact ttgtatgaag
     5221 aatatatagg aattttaatc aaaagtatat gggacaattc atttagagaa acatatatac
     5281 aagataagag taaatttctt ttgaaaaatg aacaaactgg taagaaaaac tttaatttaa
     5341 gaccggatat agttttatat gacttaaaaa atgaatatga aattataatt gacactaaat
     5401 ggaaagctat tgaagtagat tcaaatgttt tttataggtc aagtgatata taccagatgt
     5461 atgcatatat aacagcatat gaaaatgcaa aaagatgtat attattatat ccttgtattc
     5521 aaaaagataa gaattatagt tcctggaaat tatcagaatc atttaagggt aagtttatag
     5581 aagcaaaaac
//
LOCUS       DAYASV010000185         5554 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYASV010000185 DAYASV010000000
VERSION     DAYASV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.190"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            240..1067
                     /locus_tag="ACGI68_003513"
     CDS             240..1067
                     /locus_tag="ACGI68_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HFH0139916.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2092..2556
                     /locus_tag="ACGI68_003514"
     CDS             2092..2556
                     /locus_tag="ACGI68_003514"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0139917.1"
                     /translation="MNLNKVKFTEEHAIEVTNWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDKRKNFISFINDNKELIGFINLLDEGSSVFFGIGMKPNYCGMGIGKEIIGLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKVQKTHLGDGEFFVMRYQ"
     gene            complement(2823..3467)
                     /locus_tag="ACGI68_003515"
     CDS             complement(2823..3467)
                     /locus_tag="ACGI68_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139918.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMVPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3397..4686)
                     /locus_tag="ACGI68_003516"
     CDS             complement(3397..4686)
                     /locus_tag="ACGI68_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139919.1"
                     /translation="MNLFIKECKNILKSMVYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4683..>5554)
                     /locus_tag="ACGI68_003517"
     CDS             complement(4683..>5554)
                     /locus_tag="ACGI68_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139920.1"
                     /translation="EMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTTF
                     MRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVLS
                     DIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEPT
                     AGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEAL
                     AKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPTI
                     EDGYMLIMGGDNI"
BASE COUNT         2010 a          769 c          696 g         2079 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 atggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattattat
     1141 aaaatcagag tgtggaaatg tttatgtgta ttctttaaat aaaaatttcc acattctgta
     1201 aggttattat acttgatata taaaattcaa tttaggaaca tagtctaaca tttataaaca
     1261 tattaaggta atgatttctc ttggtaatta ttctcatatc ttattctttg attatattta
     1321 tcttattcct agcccttatg tcatattata tttcttatct tattacttat aattagcatt
     1381 aacttatcta aaatcttgta tttttttaat agtaaagttt acatctatat taaaaagttt
     1441 acatctatat taatttcata aaacaatcaa ggtagtcaag agattcaact ttacttgatt
     1501 ttaaaaattt aagtgaattt gttcttatac acatcttttt aaaattatat ctgtagaaaa
     1561 tatcatttag agctataatt aaattaactt attaaatttt agaaaattta ttaaaaaata
     1621 taagttaaat taatattttt aaagattctt ttttgtaact actagaatag taatttagta
     1681 gttattgtat ctctaaagtt taagcatgga tagatataca tttgataatt tgcctataat
     1741 ttacctacta cttggattat tggagcaaat acaatatatt aataataagt tcctaaaacc
     1801 aataaattaa ctaatacaca taaagatatt gatgttgatt atacaactat aagaaacgga
     1861 tttaacagaa tttaatcaag ctaagaaatt atatatatat cgtgatgttt ttgaattaga
     1921 aggtaattca aaattagttt atagtcttct gtcaatccaa taaaaagtac agggtacaag
     1981 aattgttagt aaaattttta aagattttat ggataaatat aaatagtttt acttattttc
     2041 taaaacttta aaataccatt aattacctta aatattttaa taggaggaca tatgaattta
     2101 aataaagtta aatttactga agaacatgct attgaagtta ctaattggaa atatgaaggt
     2161 aaatattcaa tatacaatct acctccatgg gaggaaatta aaaagaagaa tttttcacta
     2221 gcaaaagaag ataaaagaaa aaattttata agttttataa atgacaataa agaattgata
     2281 ggttttataa acttattaga tgaaggaagc tctgtattct ttggtattgg tatgaaaccg
     2341 aattattgtg gaatgggaat tggtaaagaa ataattggcc taggattaca agaatgtaga
     2401 aataaatata gtactaaacc aattgtatta aatgtaagaa cctggaacat gagagctgta
     2461 aaatgttatg agtctcaagg atttaaaata gttgagacta aggtacaaaa aacgcattta
     2521 ggagatggag aattttttgt tatgaggtat caatagttta taacaatagt cttattgtta
     2581 atacaacaat taaaatgaaa aaatacatat gtgtaggtca tagcaataag tgtctttgaa
     2641 atataaattt gagagctttt gataaaattt tgtatacata tatattttat actacggata
     2701 tataaatcta tctacatata taaagagatt gtaagtaggt ttgtgtatat aaaaatagat
     2761 ttatatttca taaatgtact ttttatttgt attaccttta ttttatctaa gctctcattt
     2821 tattatctaa tcttcctttt tactggtttt attaagagag atgcaattat caacacaaca
     2881 cttccaagta tcagccaata tttctcatca aaactacctt gactcataga cattataaaa
     2941 aattttccaa catacttgcc tttagtcatc atatttaaaa ttaaatatcc tatagatatc
     3001 atataaccta tgattgtatt attaaataaa tttgagaaaa acattcctaa acttcctata
     3061 aacatagctg atgcgaaact tccaataata tactcaacta ctggaaaatt acatccaaga
     3121 tatattaaaa catttacaaa acctatgatt aataccacta tacctataat tgacatcagt
     3181 atcctaatta attgtacaat aacttgagat aatgcttttg aagatactat ctcatcaatt
     3241 tcttttgatt gctctagcga aaatacaggt accaacatta ctatccctat tattgcaaca
     3301 taattttcta aacatatagc tgttgaaatt ctgtctaaat tagctactcc ttgtattaga
     3361 ggaaccatac atagcaatat aattgaacat attaaattag ttaaagtgct taatttgaag
     3421 ttttctttta ttagaactaa acagttttga aaactttgcc caaacatcaa gattccccct
     3481 tcttttttgc tcatataaga atatagctaa caaaacaagc accaatgctc ccaagcttat
     3541 cacaattcta ttaatcataa tttgattgaa gttttgtata ataatatccc catttaatac
     3601 actattatgt cttatcatta gccccattgt tgaataatca cctttgagtc ctgtagaatt
     3661 tatactgtaa agccaccaaa ttaattgcac aataattgct actggtgtat ctgttaatat
     3721 agtaatactc attcctacag ctgtaactat cataagagtt ggtagtatcc accataatgt
     3781 atattttata aatgctaagt tatcaataga tataccttta tctttagcaa agaaaatcaa
     3841 aggaagtaaa tctttcatac ctaaaattaa tacaggcaat atcatcataa agacaagtgc
     3901 aaaatatctt gataaaacta ttttgcatga agatacttgt tttgaataaa taatctctct
     3961 cattcttgaa cgcttatctt taagtgacat aaaaactgcc acaaatactg gaaatatact
     4021 taaaactata cctatataat cgcaaaaaat tcttgcatac ccattactaa ctttatcttt
     4081 ttgtattgta tagttgtaat tcttcatagc ttcatcatag gtaactggaa ctactccaaa
     4141 tctttgtaac ttgtcaaaag catatgcaga ccctcctcct aaaatgtcgt ctactctttg
     4201 cattatttct tcaaatcttt caagagatac tttagaataa tctattttta tactttcatc
     4261 agttttccaa agtgaattta tttcgctaat acttttacca gttatttctt ctatatattt
     4321 ttctatggtc ttcgttttat tttggtctaa ttttatatac ttaactaccc ctgttggata
     4381 cgttgtatat gtgttttttt gatattctct aattaggttt atagttgcat ttggaatgac
     4441 cttttctgtt ttcatagatt ctttttgacc atatggatat tgattgtgta cagaataata
     4501 ttcctcaata ttctctactt tttgtggagc aactaatgga tttccatcat taggaatatt
     4561 tttacttaca tcatttgaaa ctgaacttcc aagttgagaa atatagaaaa aaactaaaac
     4621 tacaataaaa atataatata ccatactctt taatatattt ttacattctt ttataaatag
     4681 attcatatat tatctcctcc cattataagc atatagccat cttcaatagt tggattacaa
     4741 tttactgctg tctcaaaagg tttatcatct gatataatcc taagtatagc ctttccacca
     4801 tgagtcaaca ttcctataac tataaatcta ctttttatat tttcaatgtc ttttttatct
     4861 acttcaatac tatatacttt cccttcagct agttttgcta atgcttctgt tgagccattg
     4921 tatatgactt ttccactatt caatatagct acgttttcac atgtagcttc tatatctcca
     4981 acaatatgtg tagataaaat aacgattcta tcatttgcaa tttcgcatag taaatttcta
     5041 aatcttatac gttcttctgg gtcaagacct gctgtcggct catctactat taaaactctg
     5101 ggattatgaa ttaatgcttg tgctatccca agccttctat tcatacctcc agataaagat
     5161 tttactttca cattttgata attagtaagg tttactttgc ttaataactt tgctataatt
     5221 tttcttcttt ttcttagtgg tatatccgat aaaactgcta aataatccat tgctttatat
     5281 actgtcatgt taggatacat tgaaaaatct tgtggcaaat aaccaaccat atttctaatt
     5341 tccttagtat tctcaatagg aactccacac atatgtattg aaccttctgt ttttgataat
     5401 aaagtggcta aaattctcat aaatgtggtt tttcctgctc catttcttcc taaaagacca
     5461 aacataccac tgtcgatttc aagatttata ttatcaagag cttgtttttt cccaaatctt
     5521 ttactaagat tggatactgc tattttcatt tcca
//
LOCUS       DAYASV010000186         5533 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYASV010000186 DAYASV010000000
VERSION     DAYASV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.191"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="ACGI68_003518"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="ACGI68_003518"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..1569)
                     /locus_tag="ACGI68_003519"
     CDS             complement(385..1569)
                     /locus_tag="ACGI68_003519"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFH0139921.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPVFIDTKKENGFKLTKDELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1942..4293
                     /locus_tag="ACGI68_003520"
     CDS             1942..4293
                     /locus_tag="ACGI68_003520"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HFH0139922.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4315..4854
                     /gene="nrdG"
                     /locus_tag="ACGI68_003521"
     CDS             4315..4854
                     /gene="nrdG"
                     /locus_tag="ACGI68_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFH0139923.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAREAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2156 a          628 c          891 g         1858 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc attccgaaca cagaagttaa gctctctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg taattttttt
      181 gttataataa atttagaatt taacttatat agtttaattt gataatttga tgaaaaaaat
      241 aaaagactta ctgattttta aatctatagg tcttttattt tttatctaac atttaaatta
      301 agttgttgaa tatatcttat attacttacc ttatatatta attaccatta atacattata
      361 tctaattact attcatctaa attattatgc cattatttta tatacaaatt cttttaattt
      421 atctaatcca gataagaaag tattttcact gcaagcatag gatatacgaa tatatttatc
      481 cataccaaat gctatgccag gaacaactgc aacattatat tcttctaaaa attgattaca
      541 aaattctata gaaaaactat ctttatattc aaatttctca gatactttag aaagatctat
      601 aaatatatag aatgcgccat ttggattcac atatcctaca ttatcaatag agtctagttt
      661 tgatttaatt aaatctcttc ttgatttata tgttttaacc atattgtcta tgtcaattga
      721 gcaatctttc aaggctccat aggctatgta ttgtgctgtt agacttgggt gagatataag
      781 atgtccttgt atagaactca ttgctttagc aatcgtttta ttagatgcag tatatcctaa
      841 tcttaatcca gtcattgcag ctgacttaga aaaaccattt atagttattg ttatatcttt
      901 agcttcttcg cttaatgatg caattgatgt aaattcacca gtgtagcata ttttttcata
      961 tatttcatct gctaaaatat atattttatt ttgtatgcat acatctacta tttctattaa
     1021 ttcatctttt gtataaacac taccagtagg atttgaagga ttgtttatta caagaatttt
     1081 tgttttatct gttatagatt tttcaagttc atcttttgtt aacttaaatc cattttcttt
     1141 tttagtatca ataaaaactg gaacagcatt aactaactta accatttcag gataactaac
     1201 ccaataaggc tttggtaata agacttcatc tccttcgtct gttaaagcta gtaaagtatt
     1261 tgttattgag ttttttgctc cacttgatac aactatttca tctattgaat agttacagtt
     1321 attttcttca ataagttttt tacagatttc ctctctaagt atttttaatc ctggaactaa
     1381 atcatattta gtgtaatctt tatttaaaga atcaatacca taagattttg cgttattagg
     1441 tacattaaaa tcgggttctc ctatactaag atttattact ttaactccat tactttccat
     1501 ttctttaact ttagaactaa tacctattgt gtaggatggt gttataaagt ttagtctttt
     1561 tgataacata aaatgcctcc ttttaaatta ttatttatat tatacgacaa atttaaagaa
     1621 tttacataag gaaatttgta gatatataca ttaatatata taatagccta cactttttgc
     1681 ctaaaattag tgtaaaaaaa agagaaaaaa attttaactt tcaaagttgt tagaaacgct
     1741 actgttcaaa atatccacat agtatttgca atgatttgcg caagtttgac tattatgtac
     1801 aaatttggta atcttaatat agcaattatt aagataatcc ctttaactta gttgaagggg
     1861 atttttttag acttaacata ctacatatag tggtatgggg attaataaaa tacaatatat
     1921 agtaacagga ggttttttta attggttgaa ttcgtaaaaa aaagagatgg aagagtaatt
     1981 ccatttaatg aagatagaat aacaagagct atatttttag cagcaacgaa tgttgcagaa
     2041 agagaaggaa tagtacctga ttataaatta tctgagcaat taactcaaga agtaataaag
     2101 tttttaaatc ataaatactc agaatcagtt ccaagtgttg aagatataca agattctgta
     2161 gttaaggttc ttatagaaac tggccatgca aaaactagtg aagaatatat aatatacaga
     2221 actgaaagaa gtagaataag aaattcaaaa acaagattga tgaaggcaat agaggaaatt
     2281 acctttgaag atgcagaaga tgctgatata aaaagagaaa atgcaaatat taatggtaat
     2341 acagcaatgg gaactatgtt acaatatgga agcactgtat ctaaagaatt ttgtaaaaca
     2401 catatattaa aaccagagca ttcttttgct catgataatg gagatataca catacatgat
     2461 atggattttt taaatatggg aacattaact tgttgtcaaa tagatgttaa aaaactattc
     2521 aatggtggat tttcaacagg acatggcttt ttaagagagc cacaagatat aataagttat
     2581 ggtgcattag ctgcaattgc tatacaaagc aatcaaaacg atcaacatgg aggtcaaagt
     2641 ataccatttt tcgattatgg attggctgaa ggtgtatata agacatttaa gaaattctac
     2701 ataggaaatt tagctaaagc attgaagtta tttaaaggaa ttgaaaataa tgatgtgata
     2761 aagaacatag tatataatac agagaaagaa acaaatcaaa aagttggtct taagagagat
     2821 gaattgtatc taaacttaga aaaagagaaa ttaatacaga cttttgatat agatgatgaa
     2881 cttgtaaata agatgcaaaa ctttgcattt gaagaatcgt atagagaaac tgataaaaaa
     2941 acatatcaat ctatggaagc atttatacac aatctaaata caatgcactc tagagcaggt
     3001 gcacaggtac cattttcaag tgtaaacttc ggaacagata cttctgaaga aggaagaatg
     3061 gtaactaaga atcttctatt atctcaagaa agaggtttag gtaatggaga gacaccaatt
     3121 tttccaattt taatatttaa ggtcaaagaa ggcataaatt taaatcctga agaccctaat
     3181 tatgatttat ttaaattatc atgcagagta tcagctaaaa gactattccc taattttagc
     3241 tttttagatg cgccttttaa tgcaaaatat tataaaaaag gtgaaccaga tacagaggct
     3301 acatatatgg gttgtagaac aagagtactt agtaatgtgt gtggttcaga aacagttagt
     3361 ggtagaggaa acatatcttt tactacagta aatttaccaa gattgggtat aaaacatggt
     3421 attataaata atgaaaaggc taatttagat ggtttctttg aagaattaga tgagaagata
     3481 aacttaatta tagaacaatt attggagcgc cttgaggtac aaggaaataa aaagatgaag
     3541 aattttccat tcttaatggg gcaaggtgta tggaaaggtt ctgatgattt aggaccagaa
     3601 gatacactaa aagaagtaat aaaacaagga acattgacaa ttggatttat aggtttagca
     3661 gagtgcctta ttgctcttat aggtaaacat catggagaaa gtaaagaagc tcaagaatta
     3721 ggattaaaaa tagtgtcaca tatgagacat aaaatggatg aagcaacgga caaatataaa
     3781 ttaaatttct ccttaatggg tactccagca gaaggtcttt caggtaggtt tactaaaata
     3841 gataaaaaag tttatggaga aattaaaggt atcacagata aagaatatta tactaattca
     3901 ttccatgtac cagtttatta caatataagt gcatatgata aaattgagat agaagctcca
     3961 taccatgaat tgactaatgc aggtcatata acatatgtag agttagatgg agatccatct
     4021 gataatttag aagcttttga aactgttata aaagctatga aagatttggg tataggatat
     4081 ggaagtataa atcatcctgt agatagagac ccaatatgcg gtttttcagg tgtaataaca
     4141 agtaatatat gccctgtatg tggaagaaat gaagatgaaa gtgacatcaa attcgaaaga
     4201 ataagaagaa taacaggata cttagttggt acagttgata gatttaataa tgctaaaaaa
     4261 gcagaagtaa gagatagagt aaaacataga tagttttatt tgggggaata agttatgaaa
     4321 ataagaatgt catctactat aagttatgac agcatagttg atggaccagg attaagaatg
     4381 gtaatatgga ctcaaggatg tattcataat tgtaaagagt gccataatcc tcagacacat
     4441 gatttatgtg gaggatttta tatggataca gaagaaatta taaataaagt caaatcattg
     4501 aaattacaaa aaggcattac gctatcagga ggagaaccat ttttacaacc agaaccatta
     4561 gaagaaatag caagagaagc taaaataaat gggttagatg tatggtcata tactggattt
     4621 acgtttgaac aactgttaga taaaaaaaat agagcgtatt ttaaaaattt aaatttacta
     4681 aagcaaattg atattttagt ggatggaaaa tttatagctg aaaaaaaaga tataagctta
     4741 aagtttagag ggtcatcaaa tcaaaggata attgatgttc aaaaaagttt gaaatataaa
     4801 aaagtgtttt tggttgagca atatatgaag gatgatttat ctatagcaga ataaaatata
     4861 aataaacagg attaaatata tgcatatatt taatcctgtt tattttcata taatatttca
     4921 agcttgtata atgtaacaat taaaccaaaa gactcattgt atatttgaaa ttaagttttt
     4981 tatagtttat aggtgtttta tgtggattac gaatgatata gtattgatac attatttaat
     5041 agattcttat gccaagaaac aattaattta tagaaattct taattgttac aatataaata
     5101 tctagataag ttataagata ctgataaata atttataaag tttattaatc taatattgtg
     5161 tgtgaatatt aagtggtcaa gtagtctaaa aaatgaaaaa gatttaaaaa agtgttttaa
     5221 ctaaacacat aagtgttaaa ttcatgtata agtataggtt agatacataa gaaataatta
     5281 tgtaatatct atctttgtaa aaaaatatta aattttgtaa tttgtggata aatatgttta
     5341 taatgtggat aatatttaat gtaataagtt aaagcaatat gaatcaactg tttataatgt
     5401 ggataatatt ataaatgtat gaataattgc agtatttaaa aacaaaaaaa gttaaaaaag
     5461 ttgttgactc taaaagaaaa agttggtata gtaatacttg tcctcaagaa aagggcaaac
     5521 aaataaagca taa
//
LOCUS       DAYASV010000187         5360 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYASV010000187 DAYASV010000000
VERSION     DAYASV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.192"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            212..1675
                     /locus_tag="ACGI68_003522"
     CDS             212..1675
                     /locus_tag="ACGI68_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFH0139924.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1731..2867
                     /locus_tag="ACGI68_003523"
     CDS             1731..2867
                     /locus_tag="ACGI68_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFH0139925.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3103..4218
                     /locus_tag="ACGI68_003524"
     CDS             3103..4218
                     /locus_tag="ACGI68_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HFH0139926.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTIMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLDPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4370..5239
                     /locus_tag="ACGI68_003525"
     CDS             4370..5239
                     /locus_tag="ACGI68_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HFH0139927.1"
                     /translation="MDYLKYFEKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSRRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2069 a          545 c         1002 g         1744 t
ORIGIN      
        1 acacgtcgtt aaaaatttta taaatttatg tttagatatg attaatactc ttgattttag
       61 aaataaatat ttgtagattg tagattaaag ataatataaa taaatagaag gtagattttt
      121 tagatatatt aatataaaga ttatataaaa tattgaaata tagctgttta aattattgtc
      181 tataaatata aaatgaatgg tggtgagtta agtgaccgat ttagttaaca ccattaatgg
      241 aattgtttgg tcacccgtat tagttgcaat gtgtttgtgt ataggattat atttttcatt
      301 gaaattaaaa ttttttcaaa taagagatgt aaaagaaatg tttactctct tgcttgaggg
      361 agatagttct catgttggta tatcatcatt tcaaggtttt gctacatcta tggctggaag
      421 aatagggact ggaaatatag ctggtgtagc tgtagcaata gcaatgggag gtccaggagc
      481 attagtatgg atgtgtataa tggctacatt aggttcagca actgcatttg tagaatctac
      541 tttagctcaa atatataaag atgagcatga tgggcagtat agaggaggac ctccatatta
      601 ttttgaaaaa ggtcttgggt ggaaaaagtg ttcagtttta ttttcaatag ttgcaataat
      661 aagttataca ttttttatgc ctggtacaca atcaaacact ttatatctag caatgaatca
      721 agcatttgga ttttcaaaaa tgacaatagc agtagcatct tctattgcat tagcgataat
      781 aatttttgga ggaattaaaa ggattggttt atttgctgaa aaagtagtac ctataatggg
      841 tggagcatac cttattatga cattagtaat aattataaca aatattagtt tagttccaga
      901 ggctattgta acaatgttta aatctgcatt tggatttggt tcagtttttg gaggaatgct
      961 aggttcagca atatcttggg gagtaaaaag aggtatatac tcaaatgaag caggagaagg
     1021 ttctgggact tatggtgcgg cagcagcaga agtaagtcat ccagccaaac aaggattagt
     1081 tcaagcattt tctgtttatg tagacacatt attaatatgt atggctacag gtattatgat
     1141 tgtaataaca ggaatgtata atgttacagg tgtttgcaca gcactaccag gtgtagaacc
     1201 gggaccacaa tatgtacaga gtgcaataaa tacattacat ccacaattag gtgatatttt
     1261 tatagcaata gttttattat tatttacatt tacaacttta ttagccttct catatatgat
     1321 ggagactaat gtatcttact tagtaactag aattaaagca gaaaaacatg aaaagtacat
     1381 aattaatgtc tgtagattta tgcttatagc atgtgcagca atgtgttgtt ttatagaacc
     1441 acttacaagc tgggcattag gtgatatatg tataggtgta ttaacatata taaatttaat
     1501 tgccatcttt acactaaaaa aaccagctat aaaggcatat tatgattata taagacagaa
     1561 gaagatggga ataaaaagga gtgatagaac ttttaaccct atagagttag atataagaaa
     1621 tgctgagttt tgggaaaata gatacttaaa agaggataag catgagttaa attaaagtta
     1681 taataaaata taaaaattac taaataaatt tagtgggagg taagattggt atgaaagaaa
     1741 gaataataca agtggctgat tcaaagaaag aaaaaattct agggctatgt caatctttat
     1801 atgatgaacc agaaattgct ttgcaagaat ataaatctgc taaaaaaata tcagaatttc
     1861 taagagaaga gggttttgat gttgaggaaa acttggcagg tatggctaca gcttttaaag
     1921 caaccaagaa aaatggagat ggtcctaaaa tagcatttat agcagaatat gatgctcttc
     1981 caggcaatgg acatgcatgt ggacatcatt taattgcatc tatgggagtt ggagctggaa
     2041 tagctctttc aagtatactg gatacttaca agggagaagt aagtataatt ggaacacctg
     2101 ctgaagaaac tggagatgga aagccttact tgatagaaca tggtgtattt gatggctatg
     2161 atgcagcaat gatgatacat ccaaattcaa aaacttgtgt aacacctgaa attatagcaa
     2221 taggaggtct agatttcata tttactggaa aagcatctca tgcaggagct aagccatata
     2281 acggaataaa tgctttagat gcagtagtac tactatataa taatataaat gcactaagac
     2341 aacaattagt agatggaacg agaatacatg gtattatatt agaagcagga actgctgcaa
     2401 atgtaatacc tgatatggga aaagtaagat tagagataag ggcaaaagaa caaaattact
     2461 ttgatgaagt agtagaaaaa gtgaaaaatt gtgctagagg tgctgcaata gccactggat
     2521 gtgaacttga attttatcac tttgaaccaa cttgccaagg attaaatgaa aataaagttt
     2581 tggtagacat atttacaaag attatggagg aatttggaat atatgaagat gaacaagtaa
     2641 tgttaggttc aacagatatg ggaaatttaa gtcaaataat gccatgtata catcctttga
     2701 tgaagttttc tgaaaatgga gaagaactac atacaaaaga atttttggag gcttctataa
     2761 attcatatgc aaaagataga gtaattgatg gtataaaaat attagcttta acaggattta
     2821 atttatttga aaatccagaa ttattgaaaa aaatgaagga agagtagaac ttagactatt
     2881 tttaattata ctctttaaaa taagaagagt gtctcaaaat gaacttttta gtttatgaga
     2941 cactcttttt tgtatgaaat taaatatatt tttattattt taaacatgaa aaagaggctt
     3001 atctgttttt atatgacctt ttttaacttg tagcacaaaa tatcatgaat gattacaatt
     3061 cgaatgataa aatcgattat aagatatata ggaggggtac atatggaaac tttagaagta
     3121 agaaatattg aattgagtgg tacaaattat gagataggat acagactagg agaattggtt
     3181 gctaatatgc cagagataat agaaggtcag attaataaat ctaatctggt aagcaaaaaa
     3241 gaagaaaaag agatgataga gttgtttgat aaatattgtc caggtcttaa tgaagaattg
     3301 caaggttttg ctgatgcaat tcaagtgaat tgtaatcaaa ttttatatta tactatgact
     3361 tatcttaaac caggttgtag tcaagttgca ttagcaccag aattaactga aaatgggcat
     3421 gtactatttg cacgtaattt cgatttctca cacaatatgg aggattttgt tttatgtaaa
     3481 actaaggtaa atggaaaata tgcacatata ggtactacca ttatgcaatt tggaagaggt
     3541 gaaggtatga atgaatgtgg cttaggtgta agtcagtcat catgtggtat tcctgtaggc
     3601 aattcagatg gtttaagaaa accagcaatt gtaggtttac agttttgggc tgttattaga
     3661 tatttgttag aaaattgcaa agatgtagat gaagcattag aatatttaaa agatatgcca
     3721 attgcataca atattaattt attgcttgca gataagtctg gaaacattgc acttgtggag
     3781 actttagatg ggaaaaaaga agtaaatata atcaacagtt tagaaagtaa aagagaatat
     3841 tttttacact ctactaatca tacacatatt gataaacttc ataaattaga tccacaatca
     3901 atgaaaaatt ctattcatag atataaatta attaaagaat atataaataa gtctaaaaaa
     3961 attggagaga aagagttaat gaatttattg tcttcaaaat atccaaatgg cttatcttgt
     4021 aattactata atgatttttt tggaacttta aaaagtatag ttatggattt aaatatagga
     4081 aagtttaata ttttatgggg tggactagag aataaatggg agagctacta tttaaaaaat
     4141 gacattaaat ctataacaca gaggataaac ataaatattg aaaaagcacc atctgacttt
     4201 tttgatttca ttgaataaaa aatgtattta attattctaa ataattagga catataaatc
     4261 aactaaaagt ttttatttat cgtattgaat tagttataat attttagtat agtaaaaaat
     4321 agtgctattt tacatattta aagagtttaa gtagaagaga ggtgccatat tggattactt
     4381 aaagtatttt gaaaaagctg taatgtatat tgagaataat ttacatgaaa atataactgt
     4441 aaatgatgtg gcaaaagaaa ctggatattc ttattatcat ttaacacgct tatttaagtc
     4501 tatgtttggt gaaagtgtag gaagttatat aaaaaaaaga cgattagtaa gtagtacaaa
     4561 agaactttta tatagtgata aaaaagtgat agatatagct attggtagtg gatttgaatc
     4621 ttcagaagca tttagcagag catttaaatc tatatacaag gttagcccta tagaatatag
     4681 aaaaaataga attgatgtat ttgttggtaa aaagaaaaaa ttagagttag attttatgag
     4741 acatcttgtt ggaaatataa cgattaaacc agttataaaa gaaatagaaa aaataaaggt
     4801 aataggtata aaggataaag taattttaga aaataattca ttacctgact tgtgggagaa
     4861 attcagaaaa gtacatcata ttgtcccaaa tactttgcca tcaaggagag tttttggaat
     4921 ctgtgaagca atctctgaaa tacacctagt tagtgaaagt atggagttta atgagataat
     4981 aggacttgaa gttaatagtt atgatgtgat tccaaattca tttgtttcaa aaactattaa
     5041 ggaaggaaag tatgctatat ttactcatac tggtagctta gataatttag acaaaactta
     5101 tgaatatata tggggtactt ggtttttaag ttcaaaagaa gaacttgata taagagatga
     5161 ttttgaagta tatgatgaac gatttttagg tcctagtaat gtgaactcac aaattgatat
     5221 ttatatacct ataaaataat ctataagaaa actaaaagat aatttgatta agaaatagat
     5281 gttcgtaaaa gagcatctat tttttatttt tataagattt agataatttt aataataaaa
     5341 gaaataaaaa taagttctta
//
LOCUS       DAYASV010000188         5273 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYASV010000188 DAYASV010000000
VERSION     DAYASV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.193"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            83..283
                     /locus_tag="ACGI68_003526"
     CDS             83..283
                     /locus_tag="ACGI68_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139928.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(490..1080)
                     /locus_tag="ACGI68_003527"
     CDS             complement(490..1080)
                     /locus_tag="ACGI68_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HFH0139929.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(1083..1958)
                     /gene="dpsA"
                     /locus_tag="ACGI68_003528"
     CDS             complement(1083..1958)
                     /gene="dpsA"
                     /locus_tag="ACGI68_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HFH0139930.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(2034..3425)
                     /locus_tag="ACGI68_003529"
     CDS             complement(2034..3425)
                     /locus_tag="ACGI68_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFH0139931.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDIINIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(3429..4565)
                     /locus_tag="ACGI68_003530"
     CDS             complement(3429..4565)
                     /locus_tag="ACGI68_003530"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HFH0139932.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(4597..5142)
                     /locus_tag="ACGI68_003531"
     CDS             complement(4597..5142)
                     /locus_tag="ACGI68_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFH0139933.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT         1766 a          870 c          623 g         2014 t
ORIGIN      
        1 ttcatgattt ttatataata tgaaataaaa tatatttaat attatataga taaatttgaa
       61 gtttataaga aggaggataa gcatggaaaa attaaaaaca attagcgttt tttctttgat
      121 tataagtgta attttaacga ttggaggtat tggaatagta acttattatg tagataattt
      181 gtttattaga ggactatcag tatttgtttt aattatgtcc tcttcttttg tatcaacaac
      241 agttagattg atatttgaag agtcaaaacg atataaattc taatttaaaa attggattaa
      301 gtaaataatt ttaatatagc aaaaattacg gagattagct caaaatagac tttttagtcc
      361 atgaggcatt tttttgtatg aaattaaata tattttcatc atttcaacaa gaaaaagagg
      421 cttctctcta ttttgagaca gcctctttgc tagtattgta tctaatacat aatattataa
      481 gaaccaattc tattgatgtg gactttttat aacaggctgt agctgtttgt tctctagagc
      541 aagttctata gttggaatta ttagctcagt atgtgcaatc atagaattag gctttgcttt
      601 gcaattatct tgaccaaaag gtacaaaata tatattttta gaattaagga gaatgccaat
      661 atttttgaaa ttaaagctta gagcatcatt cgttgatatt gatattacta gtggtttatc
      721 attacgaaga tgaccttttg ctgccattaa tactggtgaa tctgtaatac catttgctaa
      781 ttttgcagca gtatttccag tacaaggagc tattattata atatcagcaa ttcctttagg
      841 tccaaatggc tctgcctctt caatcgtaac tataggttta tttcctgtaa gttcatatgc
      901 ttttttcata aactcttcag aatttccaaa tctacaatcg atattttgtg atacatctga
      961 aaatatagtg tgtacattag ctccttcttt aaccaaattc tctaactcta taaatatttt
     1021 atcataagta caaaacgaac cagtaaatcc tactccaatt gtaagtcctt caagtctcat
     1081 ttctaatccc ttctttcatt tataatattt tctatagcgt taacaagaat ttcaccagat
     1141 gtctttggtg aatatttacc aggaagccca aggcaaagag tcgcattaat ccctgcttct
     1201 ttaacaacag aataatctac tcctccagga gaagaagcta tatctataat agttacttct
     1261 tgtgatactt tttctaacct ttctttcgtt aaaatacact ctggtatggt gttaaaaata
     1321 taatcaaatt gtaaaagttt ttcatcaagt tgacttaaag aaatactgga atatccaaat
     1381 gcattagccg tacttcttgc atcattattt cttgcgccaa cacatacatt tgcatccaaa
     1441 gcgtataatt tttttgcaag tatttgtgca catcttccat aacctaatac taaacagtta
     1501 ctttgatgta tattgattat acttctttta atagcttctg ctatagcccc ttcagctgta
     1561 gcaactgcat ttaaaatagc aacatcattc attttcataa aatcataaat ctgaatagag
     1621 tgttgctcgc aaaatttaca aactttagtt ggtatgttac ctccaaataa tatttgatta
     1681 ggagataaat taattagcaa gttatcaata gtagcatcaa gacaatcatc cgtattttca
     1741 atattaactt gattctttgt aaatggaatt ggacataaaa taatggaact tttggatagt
     1801 gcttcagcta gtgaagttga ttttccaacg attccattta taatttcatt tgatatccca
     1861 taagtgacta cagaataccc tttattaatt agttttttta ccatgtacac ttgtcgcagg
     1921 tctcccccaa taacagttat atcgtattga tttgacatat tgtaagcctc ctttactata
     1981 tacaatatgt ttttatcaat acattggttc actaaaatta ctaaatatta tacttataac
     2041 atttttaata atgctaatct atctatcttt tcagaagagg tatatggaaa tgaatttaaa
     2101 aagataattt tctttgggat ttcatttttc attagattat ctttcatttt aatcaacaaa
     2161 tctttcttcg taattttgtc tatagttatt acaaataata caatttgatt tcctcttata
     2221 gtgtctgagt acggcaatac aattgcatct tcaatttgag gaattttttt aacttcattt
     2281 tcaatcttag tagaactaac tttaaatcct ccaatattta taatgtcatc acttcttcct
     2341 tcaaatatta agtatccatc attatcataa tatccaatat cattgacaga ataaggtttg
     2401 gttgcattat atactgcata ttctgtattc acaaaaattt ttccttctct aatatatata
     2461 tctattcctg gaaatggtct gcctacactt agaggttttt caattagttc atcatagcat
     2521 aaatatgtaa tataatttaa ttcacttgcc ccatagtata aaataatttc agactttggc
     2581 atatattttt ttagatttct tgcagtatct tcaaataata attgagaccc tgtaaaaata
     2641 cttacaactt tgagtactgg ttcttttaag tgtttaacca aaagttgaag ttttgtaggt
     2701 ataaggtata tgttagttac attatattgt tttattgtcc ttatccatga tttacaatta
     2761 agacctgaac ttattacaat actacctcct tcatataata ctgacattat agaatttaga
     2821 ttccctgtaa aactaagtgt tccattaaca aacaagattg attccccaga gatattaaaa
     2881 ataccatttt gtataggaaa aaatccagcc caactctcat atgttctata taataccttt
     2941 ggaacacttg tagaacctga agataaagct cccatacaaa tacttttatc taaataattt
     3001 ataagcttct tatattgata catatatata ttaaatttag gattaaaaat agaaaaatta
     3061 actttttttg ctacttcatt attgttatca tcatataaat caataacgtc catagattca
     3121 tcagatataa tgattgaaat attatttttt aataaaatat catctaatac ttttttagaa
     3181 agattataat gacaaataat aggaatattt tttgaataat taatagcaaa aaaactaatc
     3241 aactgaaaca ttatattttt agaatatatt aaaattggga tattttcacc taaagaaatt
     3301 tgctttccta gttcctcact ataactcaat atattttcat aagtatattt ttctccatct
     3361 ataattaaaa aatttttatt tttatgcctc tcttttttta ttcttaaaag ttcataatag
     3421 ttcataatct acaccctttc aagcagtaaa gctgaaccta gaccaccagc tgcagctata
     3481 gagcaaattc catatctacc cttagtcttt tctaatgctt ttaaaagatg taatgcaata
     3541 attgcacctg aagcaccata tggatgtcca tacgctaaag ctccaccaaa tatattatat
     3601 ctatctatta actctggata ctttctttga aacaacacat ctataactgc aaatgcttca
     3661 ttaaattcaa cagcagatac atctaaataa ttaaggcttt ctatctctaa aagtttatcc
     3721 attgcttgta tcgctgatgt tggacttaag gttgcatctg ttcctatcgt gcatgtgtta
     3781 ataatttttg ctttaggctt cttttttaca tgttctaaat acttcttaga acatattata
     3841 ataaatgaag ctccatcatt aataagacat gaatttgctg catttgtaat agtttctttt
     3901 cctaaaattg atggcatcct atctaataac ctttgactca tcttatcacg tataccttca
     3961 tcataggtac tattatttat aggagatata atatcttcaa gtattttttc ctctcttgct
     4021 tcttttgctc ttttatgact ttcctttacc caaaaatcca agtctgcttt ctcaatttga
     4081 tataactctg ctactctttc cgcaccttcg agcatactat tttggctatt atcatcagga
     4141 gaaaattgag caactgtata atttggatta tttgtattat atcgcttatc attcttatga
     4201 tatgtgcgca ttggttgcag agaactactt tcaaaaccac ctgcaataat taaatcacac
     4261 tgaccagatt taacctttga aaaagctatg tctatactca tcatagcaga agcacactgc
     4321 atatccacag taaaagcagg tacttcattt gacactcctg ctgttaaagt cattaatcta
     4381 gctatattcc ctcctgtccc aactgcattt ccacatataa tctcatcaat tttgtcaatt
     4441 tcatactttt taattaaatc ttttaaaaca cttgctccta gtaattcagg ttgaacaaat
     4501 tgaaaaattc cattttttag acctatatga cttctcaatc ctcctaatat aaaaacttcc
     4561 ttcatacatt acacccttcc ttatattata gatttatcat agatttcttc tgagtacatt
     4621 gttcagttta gtgcctaaaa aagatgcaat aactgacttt attgtatctc caattaaaaa
     4681 tggtactact gctgcaaaaa gtgctgcttc tactgtcatt tgattaaaat agcacataaa
     4741 tactgttccc ataaaatata taataggcat tccaacaaaa attgttatta atatatatct
     4801 cttaatatca ttatttcgtc cttttaataa actaataatt agtgctgcaa acaaaaaccc
     4861 aaaataaaaa ccacctgttg ggcttaataa ctttccaata ccagatgtac ctccaccaaa
     4921 tactggaatc ccaattaaac ctatcattat atatacacca actgtactaa atgcttgtct
     4981 tggtgttaaa atcaaagcta ttaaatttat cattattgtt tgtgctgtta ctcctgctgg
     5041 agtaaatggc aatggtatat atatgtaaga tgatatacac aataatgcta cacatattga
     5101 cattttagtt aaatcttgaa tatttacttt agtatttttc atttttataa ttcccttcat
     5161 atgtcttatt tcatttagtt tatatgtaca tatacaaagc ttctattaaa gctttgtttt
     5221 agtataaaat aaagacgttt ttttgtcaac tattataatt atttaggttt aca
//
LOCUS       DAYASV010000189         5254 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYASV010000189 DAYASV010000000
VERSION     DAYASV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.194"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(275..1681)
                     /locus_tag="ACGI68_003532"
     CDS             complement(275..1681)
                     /locus_tag="ACGI68_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0139934.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRDGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQTLNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVELDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(1726..2433)
                     /locus_tag="ACGI68_003533"
     CDS             complement(1726..2433)
                     /locus_tag="ACGI68_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139935.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            2595..3518
                     /locus_tag="ACGI68_003534"
     CDS             2595..3518
                     /locus_tag="ACGI68_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139936.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     TIMQRLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     SIKELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            3518..4279
                     /locus_tag="ACGI68_003535"
     CDS             3518..4279
                     /locus_tag="ACGI68_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139937.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFAAVEIMWCIVNSNRAITRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            4291..5028
                     /locus_tag="ACGI68_003536"
     CDS             4291..5028
                     /locus_tag="ACGI68_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0139938.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         1809 a          808 c          684 g         1953 t
ORIGIN      
        1 aaatattaac taaaaaataa aaaaaatgga attatctaaa gaatttaata accttacaat
       61 aattccaaaa tattatttct atgaaaattt tgataccatg agtaactcag ccataagcgc
      121 ataaagcaca agttactctt tactttaata aaacttgaca agaaaagaat ttatccttat
      181 tctactctaa tagagttaat ttgtcaataa agaataaaat tattttcaaa atattttcat
      241 tcttcattct tatctgttat tattttataa aatatcactt aattagaggt aattttagta
      301 ttgtttttag tccctgacta tcaatgctcg taatatctaa tttacctcta tgtgcattaa
      361 taatgccaga tactatcaaa atacctagac catgattttg acctttggca tatgcctcac
      421 ttagtccatt ttggctcttt aataaactat taattctttc ttgactcata ccttttccat
      481 catcttcaac aattatatag caaaaatcta aatcagactt agaggtaacc cttatattac
      541 atccttgttg gttatgagtt atactatttt gtattaaatt actaatagcc ctttttaata
      601 attttttatc tgcatctata ataatatctt catttagtat atcaagttca acgtaaaagt
      661 cattatccaa attattattt ataaactcag aaataagctc tctcaaaaac gcagaaactc
      721 taatcttatc acaattcaat gtttgcatat tatattcaag tttagacact aaatttaggt
      781 catttactaa ttctctgatt ttaatccctt gattgcatat aatagacact tgctcttgtt
      841 ctctatttga aaggctactg ctttcttcaa gttctccagc atatccaatt atcattgaaa
      901 gtggagttct tatatcatga gatattcctg caatccagtt agacctagct tcctctttct
      961 tcctaagttt ttcatctttc atttgcaact ctttagatac agtattaata gattttgaaa
     1021 tatcacttag tattcctttt tcttgtaaaa gtactggttc tccttttgac ataagcttta
     1081 tgccagtaat aattggtttt atagatttca ccatttttaa acctatgaat agagctatta
     1141 aaaaagtaac tattgcatta accagaaaca tacctattat taacatagga aattgttcta
     1201 tttccttaac cgtataagta aggttaaact tagtatactt gtcttttgga taacctgtta
     1261 ctaatattcc atttggatgt tcccatacat acacaggata gtcctttaaa aagaaccttg
     1321 aaaactttgc tacatctgtc aaagtataat gacttggaat ttcttttggt aaatcaaatc
     1381 cccatattct atctccattg ttatcaatta acattgccca aactttattt ttatgtagta
     1441 cttccttact agaatcatct aaaataaact ttccatctct taaaatcaaa ttatcttcaa
     1501 tcataatcaa gtttgatttt atttctctac tatcacttgt atctaaatta tcagagtatt
     1561 tagatacaaa taacatatat gaaaacagaa gaaaacctgc tatatttaat acaataaaaa
     1621 gtaatgaaat caagatactt gaacctataa atcgcctcaa tatgtacttt acactatcca
     1681 aaattaaatc actcctcttg atttctttta ttctaatatc taaaattaat taatcatcaa
     1741 cttatatcca agacctctta ctgtaataag atgtttaggt gtagatggag ttttttctat
     1801 cttctctcta acccttctaa catgaaccat aagggtattt tcatatccat acatatcatc
     1861 tccccaaatg gcttgacata gtgaatcatt agtaacaatt ctccctttat tttcatacaa
     1921 ctttaaaagc aacgaatgct ctttagctgt taaagaaatc tcttgcccat ctttctcaac
     1981 acatgcacta tttaaattca caacaacaga atctcccagt ttaaatactg gcaactcctc
     2041 ttcttctata ggcacatata cacgatttaa tatacccaca agccttaaag taagttcctt
     2101 tggtaaaaat ggctttacta tataatcgtc agcccctaag cccaaaccaa tcaatctatc
     2161 ttcatcttca cctcttgcag agagaaaaat cactggtatt ttagatgtct ctctaatttt
     2221 tctcataata gaaaaaccat ctccatctgg caatacaaca tctaaaattg cacaatctgg
     2281 ctttacattt ctaaataccc taacagcttc ctcataactt gatgctaaaa atactctact
     2341 aaatccctct tttctcagga tttcatctat catttttaat aattccttgt ggtcatcaac
     2401 cacaagtatt tttttatctt ttattgactc cataatcatc accctccttt agtataccat
     2461 atacccattt tcacaacttt gcctaaatag acttttaagg ttattgtaag gtaaatttca
     2521 gctaattgaa atgtaaccaa gttattatac tgatataagt taaaagaatc aataagcaca
     2581 gattaaaaaa ggggatgaaa catatggaaa tgataattag aacacaaaga ctttcaaagg
     2641 aatacaataa aatatttaga gtaaaggata tagacctaag agttccacaa ggagctgtat
     2701 atggtttttt gggtcctaat ggagctggaa aatctaccac tcttaaaatg cttcttggac
     2761 ttgctaaacc cacacaagga aatattaata ttttaggtaa agagttaaat gaaaaaaata
     2821 gaatatcaat tttaaaagat ataggttctt tgatagaatc tccatcatac tatggtcatc
     2881 ttacagggct tgaaaacatg accataatgc aaagactctt agatttacct aaaaaaaatg
     2941 taaatgaagc attaaaaatt gtaaggttag aaaatcaaaa aaataaaaaa gttagtcaat
     3001 attctttagg tatgaagcaa cgtcttggca ttgctatggc aataatgaag tttcccaaat
     3061 tattaatact tgatgaacct acaaatggac tcgacccagc agggattgag gaaattagag
     3121 aattaataaa atctctacca gaaaaatacg ggatgactgt tctaatctct agccatcttt
     3181 taagtgaaat tgaccaaatt gccacatcaa taggaataat aaatcatggc gaactggttt
     3241 ttcaagatag cataaaagaa cttcataata gaggtcaaag tcagattgct atcaaaacta
     3301 agaatgttgt atctgcacaa aaactattaa atactaaagg atatatgtca cttatacaag
     3361 atgggtactt gactattgaa aatttaaaag actcagatgt cgctaaaata aatggtatgc
     3421 ttgtccattc aaatatagat gtatttagaa tagaagaaca taaaaaaagc ttggaagata
     3481 tattcttaga acttactggt aaggcggtga ctttataatg aaataccttg gtctagaatt
     3541 ttataaactt aaaagaaaga agattattct gatgaccttt ttatttgcag ctgttgaaat
     3601 aatgtggtgt atcgtaaatt caaatagagc tattacacgt aatccagata tgttaggtgg
     3661 atttgaatat tcctatatgc ttatgagttt tgcaagttta aatggtctat tctttcctat
     3721 cttaatatca ataattacat cacgtataag cgatatagaa cataaaggag atacatggaa
     3781 gcttttaaaa tcttctgtga catctttaaa tagtatttat ttatctaaat ttttatgttc
     3841 tgctattttg gtatctatac ctgtattgat gcaagtgtta tctattgtgt tgtttggaca
     3901 ttttagaggg gttattgagt caatttctgt atctttactt ttaaaataca cattaggaac
     3961 aattcttgta agtattgcta tcattgcact acacctatgg attgcaactg taattccaaa
     4021 tcaaatgatt gcaattacat ttggcatgat tggtagtttt ataggtttga catcaggttt
     4081 gttttatcaa ggtattcgta gattgtttat atggtcttat tacctagagt tatcaccttt
     4141 aagttatgct tatgataatg ttttaggttc atctgtctat agaattgata tgaatttttc
     4201 tattacgatt ttagttttct taattggaat gctaatttat tatattggaa gaaatcattt
     4261 attaaaaaaa gaagtatagg agggatttaa atgttaagga agtcagtttt gggagaaatt
     4321 ctaaagttaa aaaattcttt tatatggtat gtattattag ctttgccact tataagtgtg
     4381 ttgattggtt ctggtaattt ttatctaaat caaggcatac tcaaaaaaga atggtacagc
     4441 ttatggactc aagttagtct tttttatggg gaatttttct ttcctatctt aatcgcaatc
     4501 ttttgtgcat atatttgtag gcttgaacat atgaatcata attggaataa tgtaatgaca
     4561 ctgccagtta atttaaaaaa tatcttttta tctaaactta cagtagtttc aatacttaca
     4621 gctattacac aagttttctt tgttatcttt tatatagttg caggaaatat gtttggtttt
     4681 tctggtagtg tacctaaaga attgactggt tggattgcta aaggttggat tatttctatt
     4741 tcaatagctt ctatacaatt atacatgtct ataaagatta gaagttttgc aactcctatt
     4801 ggtattagtc tttgcctttc tctatttggt atgggattaa tagtagcaaa acctgctgtt
     4861 ggtatatttt tcccttattc tatgttaggt agtgctatgg gggttataaa gcaatctgct
     4921 ttaaatgtta atgacactat agttacaata tgtgttgcat ttattactac tatagtattt
     4981 tctgaaattg ccaatagaaa ttttagaaag aatgatgtta tttcttaatg gtaaatgcta
     5041 gatatttttg ggtttgaaat ttatattctt tttagttgaa atctatatcc attttattta
     5101 aaaatatata tcttttattt gaattttata tgttttatct actcgctata taatagatat
     5161 actcctaaaa tttaaaaata tataataatg ttattattta aaattattat gtattatatt
     5221 aaaacttaat gcaaaaacaa tttttatatt atat
//
LOCUS       DAYASV010000190         5251 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYASV010000190 DAYASV010000000
VERSION     DAYASV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.195"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(104..655)
                     /locus_tag="ACGI68_003537"
     CDS             complement(104..655)
                     /locus_tag="ACGI68_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFH0139939.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            846..1808
                     /locus_tag="ACGI68_003538"
     CDS             846..1808
                     /locus_tag="ACGI68_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFH0139940.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2103..3947
                     /locus_tag="ACGI68_003539"
     CDS             2103..3947
                     /locus_tag="ACGI68_003539"
                     /inference="COORDINATES: protein motif:HMM:NF021615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFH0139941.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFKNSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4269..4853
                     /locus_tag="ACGI68_003540"
     CDS             4269..4853
                     /locus_tag="ACGI68_003540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HFH0139942.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKGLVRDYNQTDSSDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2011 a          528 c          834 g         1878 t
ORIGIN      
        1 tataatgaat ttataaagaa aaataaaata aaaaagataa atcctaatca aaggatttat
       61 ctttttgtat agaaaataat tattctatta ttaaatggca tggctaaact ggataataat
      121 ttattattac tgctttcaat gttttttctc cttgattatg gtatgtatga attgattcag
      181 ctgaaaaaca aatagagtca ttttctctaa gttgataaat agagtcattt acttctaatt
      241 ttaattgacc ttctataatc ataatatatt cctgtgattt ttcagaatga ccaacagatg
      301 tataactatg accttcttct aattccattt gaaataattc gaagtttcta gttggtgtat
      361 tgggataata acaataaaga cgataatctt tattttctga aacttgtaca tctatatcag
      421 tttttgatac aataaaagtt tcattttgag gttgttccag tattgcagta tatggaacat
      481 ttagtccact tgcaattttc caaattgtgt ttactgttgg gtttgagtct cccttttcaa
      541 tttgcgaaag cattacttta cttacgccag ataattcagc aagttgacct agacttaagt
      601 tacgttcatt acgtaatcta tttaaatttt tagcaataac agtattaata ctcatagtag
      661 ctccttaaaa tgtaaattat tgacaaatat aatttacgaa tgtataattt atttaacatt
      721 atttataatt tacaaatgta taatataatg aacgttaaat acactgtaca tccgtaatat
      781 aaattatact gcataaagag ttgtttttca acggatgata gttgagagat taagggggaa
      841 ttgtaatgcg tgtacttaat aatgttacag aggctatagg caatactcat atgttacatt
      901 tatccaaaat agcagatgaa tttggagttc aaggtaatat ttatgcaaag atggaatact
      961 taaatccggg atttagtaaa aaggaccgtg tagcattaca aatgattacc gaagcagaaa
     1021 atgcaggttt attagttcca ggtcaagcag ttgtggaatt aacaagtggt aatactggaa
     1081 caggactagc aatagcttgt gcatgtaaag gatataaatt tatagcgtgt atgtcaaaag
     1141 gaaattctat ggaaagagca agaatgatga aagctttagg tgctgaagta gttcttgtag
     1201 accaaatgcc taattcagtt catggacagg tatctggtga agatttggca cttgtagaaa
     1261 ttgaggctca aaaaatagca aaagaaagaa atgcatttag agcagaccaa tttaatttag
     1321 aagcttgtaa tcatgcacat gaaaagtata cagcagaaga aatgtgggaa caattggatg
     1381 gaaatattga tgtatttgta gattttcttg gaagtggtag tacttttgca ggatgttcaa
     1441 aaacacttaa aaaacataat tcttctatca aatgttatgt tgttgaacct gaaacagcag
     1501 caatttattc aggtaaagaa ataactgacc aagggcataa aatacaaggt ggaggatatt
     1561 ctatggattt gccattagta gacaagaaac ttattgatgg ttatattcaa gttacagacg
     1621 aggaggcaac tgatgtagca agaaaattag ctaaattaga aggtgtattt gcaggatttt
     1681 cttcaggggc aaatgtatgt gcagcaatta agttgttaaa gagcagtgaa aaaggaaaaa
     1741 atatagtttt aactcttaat gatagtggtt taaaatattt aagtactgat ttatatgaag
     1801 aattgtaata atagcacaat agttatgcaa tgttttttaa attaagtata tagatttaaa
     1861 ttataaaaag tatctcaatg taatttttga aagttataaa ggagatactt ttttattttg
     1921 taaatatgga gtatcctact tataagtatt aagctatata gatattgatg attagctatg
     1981 caataaaaaa gagtatgtgt ttttgcattt atacatcata acttggaata atacatatta
     2041 atgttctaaa tgtatttaaa acatatgata aaattagaac aaatagatac aaggagataa
     2101 aaatgcaaaa gagaatttta agaaatttta tatttacggg taaaaatatt ttaagtaaac
     2161 ttgtttttat aataaaatat aattttagta cattaatttt atttgaatta tttcataaag
     2221 gattagcctt attttttata tggccatcta ttagatatat atttgatatt ttaataaaaa
     2281 gtttacatat agtctaccta aatatggata attttataaa agttttgacc aatcccatgt
     2341 caatagttct gataatacta tcagttataa tactagcttt ttatgcattt tttgaattta
     2401 cctctgtaat tatttgtttt aataaatcga taaaatatga aaaaataggg ttatttgaac
     2461 tctttaaaat atcatttaag aactctataa aattgttata tcctaaaaat atactattat
     2521 ttatatttgt gatattaata attccattag taaatacagt tttaatttca gggtttatag
     2581 gaaaaataca aataccagag tacatactag attatataaa gtctgatata attttaaata
     2641 taatctatat gatttttctt ttaataatat atatttcggt tattagatgg atttttagca
     2701 ttcatgagat aactttaaat acagaaaatt ttaaaagagc aagaaaagaa agtgttaatc
     2761 ttataaaggg aaagattata agaacaatta tttattcatt aatattatct ttagtagtag
     2821 ctattattgg atatgcgatt tatcatagtg gaattatatt tataggcata tggactaaat
     2881 attgtactga tatggaattt ttgaagaatg tatttataaa tagatgtatt gcatttgaag
     2941 aatattttag attgatttta tcaatattca tttttatatt ggacattgga tttatctcag
     3001 caacttatta tcaatataaa ggagtaaata tttctaaaaa taatattaaa aaagagaaaa
     3061 aatctatatc aaaaattgtt tttaatttag tattaatatt attgatttct tatatagagt
     3121 cagttgcatt ttcttcacat ataaatggta tgtttaatac gtcattttca ttttatacaa
     3181 cagcaattgc tcatagagca ggagctgaaa tggctccaga gaatactttg gcagcaatag
     3241 aagaagcttc acatgctaaa gctgattatg cagaaataga tgttcaagaa actaaagatg
     3301 gagaacttat tgtacttcat gattcaaact ttaagagaac tacaggagta gataaaaatg
     3361 tttgggatgt aaattataat gaagtaaaga catatgatgc gggaagcttt tattattatg
     3421 gaaaaggtta ttatgatgaa aagtttgttg gggaaaagat accaacactc aaagaaatga
     3481 taaaatattc taaaggtaag gtgaaattat taatagaaat aaaacttaat ggacatgaaa
     3541 agggagacgt agtaaaaaaa gttgttcagt taattaaaga aaataatttt gaaaatcaat
     3601 gtatagttgc atctatggat aaaactatat tacaaaaagt aaaaaaactg gatgagaatt
     3661 tattgacatg ctattttatg gcaattgcat atggtgattt ttcaaagtta aattatgttg
     3721 atatatatgg aattgaatca acttttgtta ataaaacagt agtagaaaag attcataaaa
     3781 tgaacaaaaa aatcttcgtt tggactgtta atagtgacag tttaattgag aaaatgcttg
     3841 acctaaatgt agatagtatt attacagata atccatacct tatagagagt gcaatttatt
     3901 ggaagaaaaa tggatttatt agtcaagtgt cagattattt attttaaata ttctataatt
     3961 gtattatcca gtaaaagaga tattattaaa atcaataata tctcttttta acataaataa
     4021 ggttgttaaa ttaaggttat tcttataaaa attgtgctta tcagctataa acaaggaata
     4081 aaagtgaaaa aattgctctg agattattac agttaaaaga gttttaagat gacagagaga
     4141 tagattgtca actgtatatt taataaatct tgttatttat taaattttat ataagtaaaa
     4201 tatataaatt aataaatctt atgctagaat atatatgaaa aacaatataa atttggagga
     4261 ttaaaaacat gacagagcga gaaaaaatgt tggcaggaga actttacgat tgtggtgata
     4321 ttgagttgtt aactcaatgg cataaagcaa aaggcctagt aagagattat aaccaaacag
     4381 attcctcgga tttggaaaaa aaagaaaaaa tattaaatga gttacttggt ggtaagggta
     4441 aaaacttatg gattactgca ccgtattatg tagattatgg aaataatatt tattttggaa
     4501 ataattgtga agtcaatatg aattgtacgt ttttagatga taataaaatt atcattgggg
     4561 ataatgcact aatcgcacca aacgtacaaa tatatactgc atttcatcca actaatgctg
     4621 gtgatagatt tggtaatgcc aaagaagatg gttcatttga attttgtaaa actcaaacag
     4681 ctccagttac tattggagat aatgtttgga ttggcggggg tgctattatc atgccaggtg
     4741 ttactattgg agataatgta gttataggtg caggaagtat tgttacaaaa gatattccaa
     4801 gtaatatgat tgcatacgga aatccatgcc gtattattcg agaaaataaa taaagttaaa
     4861 ttttcttgca aaatctcaaa aaacaccttt cttactttat acatagggta ttttctaata
     4921 ctttcacatc catattcatt tgttaaattt ttgtggaatg tagtttttat cacttctatc
     4981 ttacatttcc accatttttt tataaaagtc attcctttat agatttattt ataatagtat
     5041 atattctatt tgatgtttga ataagcactt atttttaagt aaaatactaa cttttaggtt
     5101 agtattttgc tatagtatga tgattaagta agaataaaat actataatac ataaagctta
     5161 ataaatataa tttttatttt acttagatat tttcttttga ttagcaatca aaagtagtta
     5221 taattttttt aattaataag agtaacattt a
//
LOCUS       DAYASV010000191         5176 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYASV010000191 DAYASV010000000
VERSION     DAYASV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.196"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            121..1647
                     /locus_tag="ACGI68_003541"
     CDS             121..1647
                     /locus_tag="ACGI68_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0139943.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1675..2232
                     /locus_tag="ACGI68_003542"
     CDS             1675..2232
                     /locus_tag="ACGI68_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139944.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2754..3791
                     /locus_tag="ACGI68_003543"
     CDS             2754..3791
                     /locus_tag="ACGI68_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139945.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNQILTKVVNDDNEIEEKIIEEDLLISENTIKLDDKEAIINDKNIELCKKEVQVE
                     SEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAINL
                     YTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKSEW
                     SYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYDEY
                     FKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTKVG
                     RNFIRYSLRSI"
     gene            3902..5044
                     /locus_tag="ACGI68_003544"
     CDS             3902..5044
                     /locus_tag="ACGI68_003544"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0139946.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2182 a          430 c          922 g         1642 t
ORIGIN      
        1 aatataacca agcgattttt aatttagctt tttaataagc ttatttttta tactatttat
       61 aataaataaa ctccatgatg ggaaaactat tactatttaa tatgtaaagg agtattttaa
      121 atgaatgaat catattggtt tttaaactca agtccaaaag aatataacaa attaggtgaa
      181 aatataaaaa cagattgtct tatagtaggt ggtggaataa cgggattgac aactgcatat
      241 ttattagcaa aagaaggtaa aaaagtagtg cttgttgagg ctgataaaat aggatatgga
      301 acttctggaa ggaatactgg taaggtaact tgtcagcatg atatatttta ctctaagata
      361 gagaaaaaat atggattaga taaagcaaaa tcatattata atgcaaataa tgaagcttta
      421 aatttagttg aacaaataat agaagaaaac aatataaaat gtgattttaa aagggaaact
      481 tcttttgtat ttacagaaaa agaggatact ataaaggata ttaaagatga atatcgtaca
      541 tgtaaaaaaa taggaataaa ttgtgagtat catgaaacta tagaaaatat tccattagat
      601 ataaaggggg ctattagttt tactaatcaa ggtcaattta acccaaagaa gtatattgat
      661 ggattggcaa aagcagcagt aaatttaggg ctaaaaatat atgaaaatac accagtagta
      721 gaccttgaaa aaggaaaaat atgtagggtt aaaacaagag aggataatat aatagaagct
      781 gagaatgtta taatttcatc tcatagtcca tggtatgatg gattgaattt ttattttgca
      841 aaagaatatg cagagcgtgc ttatttaatg gttgcagttt tggaaaataa attagcagat
      901 ggtatgttta taagtataga tgacccatca ataactttta gacaatataa tgatggaagt
      961 gaaaatttac tgatttttgg aggaggagac cataaagttg gtcagggagg aactgagaaa
     1021 gaaatatttg atgatttaga gcattatggt aaagaagtct ttaaggtaaa agattttaag
     1081 gggaaatggt ctgctcaaga taatatgagt tttgacaatg ttccttatat tggttatatc
     1141 aacaaaagag aagacaatat ttatgtagct acagggttct ctaaatgggg aattactaat
     1201 ggaactgctg ctggaataat aataaaagat ttaataataa ataataattc tgattataaa
     1261 gatactttta atccatccag actaggtagc tacttttcaa aagattttat aaaggaaaat
     1321 gcaaacgtag caataaatta tgtaagtgga aaattaaaaa taggtagtgg agatatgcct
     1381 aaaaataatg gagaaggaaa gattgttaat attgatggaa aaagatatgg tgtatataag
     1441 gatgataatg gagattttta tattgtagac actacatgta ctcatttagg atgtgaactt
     1501 aattttaaca gtgaagaaaa aacatgggac tgtccatgtc atggttctag atttgactat
     1561 aagggaaata tacttgaagg accagctctt aagccattaa aactttatgg acatggtgat
     1621 aatgatgtta atcctaagtt attataaaat tatttttagg aaggtaaagt gtatatgaat
     1681 aaacttttaa agccaataac tatactattg tatatagttt atattttatc tgccgtattt
     1741 tttgtagcaa acaaagaata tggaagtcta ggtctagtag caattagttt agttggtact
     1801 ttcattttga gttttataaa taagcaaact gaaagattat tgagtaaaga attatatata
     1861 atgcttgctt tatttataat gttttcatca ttgttaggta cttgttttaa tttttatgat
     1921 ataaattatt atgatgattt tttgcattta tggtcaggta taatatcttg tattgtggct
     1981 ttttctataa ttaaatactt ttatacacaa agagagataa gtaaaatgag taaagtgttt
     2041 ttagttatat ttttatttat gtttagtatg ggagtagcga gtttatggga aataggtgaa
     2101 tttttaatgg atacttttgt tgggactact acacaggccg gagggttaga agatactatg
     2161 attgatatgg tagatgcttt aataggcaca attataacta ttccttttat agtaaaaaaa
     2221 tctaatatat agttttaaat tttttatatt aattataatg gatttaatta atcatgttga
     2281 gtttcaaggt ctatatgatt aattaaatcc atttttgtta tttataatta ttattgaata
     2341 tatttttaca tataagatat aaggtgatag atagaaattg ttggattatg taaataatta
     2401 atatataata ccattatggg caataaatac aaatcaaatg ataatatagt ctatttatgt
     2461 aaatgttata ttgtatccta agtataatag taagtttttt gatagtggtg tagaaagtaa
     2521 aaatctacta gcctaaattg atgtatggat tagtttagga acagtaattg catatcttaa
     2581 gtttgatgtc atgttgggct actagaaata ggtcatcttt ttcacttgaa tatcttttct
     2641 ttaaacatgg ggagtatcat atcaaaaaca tatgaaaatt aagtgattta tgagaacagt
     2701 aatattttat atgatagtat agaatagtaa taaagtatta ggaggaacat tgagtgttaa
     2761 aaaagtggtt tggtattgtg aaaaaaagac aaaaaagtga gtcagtaaaa gaagaaggtg
     2821 aagtaatatt aaaaaacgaa aaaatattat ctgaagaaaa gttgatagat gaagaaggag
     2881 ttgtagttaa tattgataat caaatattaa ctaaagtagt aaatgatgac aatgaaatag
     2941 aagaaaaaat aatagaagaa gatttgttaa ttagtgaaaa taccataaaa ttagatgata
     3001 aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt caagttgaaa
     3061 gtgaaaaaat aggtttaaat aagtttgaag gactggacca aaatgaaaat tataatctag
     3121 aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa gatttagagt
     3181 acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac ttgtatacta
     3241 aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa gttccattag
     3301 ggtacataag agaaaattta aaatatggat atatggatta ctttggagat gcaataaatt
     3361 atttaagtga agtattaaat atagatgaat actgcaaaag tgaatggagc tatctcgata
     3421 atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt agtataaggc
     3481 tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca ttaaaaatga
     3541 attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa tataagtcta
     3601 taatcaaaag aggtggaaag aaaaaagttg aattagtaaa taaaaaaggt gacattttag
     3661 aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag aatgaagtta
     3721 tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg tatagtttga
     3781 gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac acatttaata
     3841 ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa ggagggtatt
     3901 aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaatctaaaa attcagataa
     3961 tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca aagttaaaaa
     4021 aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg ttgcagtagg
     4081 agctatatgt ttaggtggag gctatgtttt tggcaaagaa gctggaagaa aattgcctgc
     4141 tacttcaaaa cactataata gcagtgaggt agtagctact attggggata aaaaaataac
     4201 aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa aaactactat
     4261 gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga ttacatctga
     4321 tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc aactagatgc
     4381 tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta aagaagagtt
     4441 tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa aagagctagt
     4501 agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag agaactatta
     4561 caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa taaaaactgt
     4621 tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga aagaagctga
     4681 agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta aaaaatactc
     4741 tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag gtcagatggt
     4801 ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta acaaacttgt
     4861 agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc agccatttga
     4921 agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc tacttataca
     4981 aaatctaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga aagctaaaga
     5041 atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa aagctatttt
     5101 tactctcaat atagagagta taaaatagct tttattattt tgcaataaaa tctatttata
     5161 tatatgaagt gataaa
//
LOCUS       DAYASV010000192         5154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYASV010000192 DAYASV010000000
VERSION     DAYASV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.197"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(4..573)
                     /locus_tag="ACGI68_003545"
     CDS             complement(4..573)
                     /locus_tag="ACGI68_003545"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0139947.1"
                     /translation="MFIVTIQEDFYMIKETHERGGYIFALLILPFINNLYLVKYDIAY
                     KIVLIIIYIYFAYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLI
                     YSFFKSLTIFTNNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIK
                     TNSKTEKFISKLLNFIVIFLIGYQFYILL"
     gene            complement(653..1357)
                     /locus_tag="ACGI68_003546"
     CDS             complement(653..1357)
                     /locus_tag="ACGI68_003546"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFH0139948.1"
                     /translation="MKKKILIPVIMSLFIISQCITSFAFTPENNKFKVKPLPYAYDAL
                     EPYIDKETMKLHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVR
                     NNAGGAYNHKFFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWA
                     WLVATKDGKLSIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVV
                     NWNGALENYKNLKSQD"
     gene            1530..3404
                     /gene="asnB"
                     /locus_tag="ACGI68_003547"
     CDS             1530..3404
                     /gene="asnB"
                     /locus_tag="ACGI68_003547"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFH0139949.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(3496..4932)
                     /locus_tag="ACGI68_003548"
     CDS             complement(3496..4932)
                     /locus_tag="ACGI68_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFH0139950.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
BASE COUNT         2000 a          614 c          741 g         1799 t
ORIGIN      
        1 caactataat aaaatataaa attgatatcc aatcaaaaaa ataactatga aatttaatag
       61 cttagatatg aatttttctg tttttgagtt agtttttata ggtagtaaag aaatgtttat
      121 ggtaatggga taaaacaatt caataccttc tttagtaata aggtcaagac acaagtgaga
      181 tagatatcct attataaacc cactggacaa acatgagaaa actatattat tgttagtgaa
      241 gattgtcaga gatttaaaaa aactataaat aagtaataag gcaattagac tgtgagtaaa
      301 ccctctatgt ctaaacctac ttccaaataa tttgtatatt aacataaatt ttttacttat
      361 gtaagaacct ctcatatcta tatctggaaa taaagaacct aaatatgcaa aataaatata
      421 aattataatt aatactattt tatatgcaat atcgtattta actaggtata gattgtttat
      481 aaaggggagt atcaaaagtg cgaatatata acctcccctt tcatgtgttt cttttatcat
      541 ataaaaatcc tcctgaatcg tcactataaa cattatattg gtaaatttag actatctttt
      601 attatattat atagcaaaca taagtttttt tcaaaaggat tttaatattt aattaatctt
      661 gagattttaa atttttgtaa ttttctaaag caccattcca atttactaca ttaaaccagt
      721 tgtcaatgta ttcatttctt ctattttgat attttaaata gtaagcatgc tcccaaacat
      781 caagtcctat tataggagtt aggtttttac ttacagggct atcctgattt ggagtagtca
      841 taatagataa tttgccatct ttagtagcta caagccaagc ccaaccagaa ccaaagacat
      901 ctaaagcaga tttttggaac tcttgcttaa atttttcaaa agaaccaaag tctctatcaa
      961 tagcttcttt taaagattca gaaggtatgg ttttttctgg cgtcattata tcaaaaaaga
     1021 atttatgatt ataagctcca cctgcattat ttcttacagt tgtagcaata tctttaggta
     1081 aagaatctaa attttgcaat aattcacata aagaataatt ataaagctca ggatattttt
     1141 caagagcagc atttaattta tcaacataag cttgataatg cttatcatga tgcagtttca
     1201 ttgtttcttt atctatataa ggttcaagtg catcatatgc ataaggtaat ggtttaacct
     1261 taaatttgtt attttcaggt gtaaaagcaa atgaagttat gcactgtgag attataaata
     1321 aagacataat aactggtatt aatatttttt tcttcataaa aatcacctcc taatatagta
     1381 tttctagaaa taaaaaaaat atgaaatatt aacagataat ttataagaat ttaatagcaa
     1441 tttcatttta aattgaataa gttttaaagc caaagacaaa actatgaaat atagaataag
     1501 actaaaggac agaaaggaat tttgattata tggacggcaa tatttcaatt tattataaag
     1561 gtgatatatc taaaaaagaa gatattaaat ttaaaataaa agaatctgta aataatacaa
     1621 gactttctag tagaatagag tataaggatg aggaaaaaac ttttgatttt gaaagattta
     1681 atgtaattga tgctatttat ggaaataaac catttgaaaa agatggaaaa gttattgttt
     1741 ttaatggaaa tatatataac tatcatgaaa taaaagaaga attaattaaa aaaggacaca
     1801 attttactac aaatgatgat attgaagtct tattagcatc ttatatagaa tttggtaaga
     1861 attgtgtaca taagattaaa ggaatgttca actttataat ctatgataga gaaaatgaaa
     1921 gtatatttgg agcaagagat ttatttggga taaaaccact ttattatata aataaggaaa
     1981 atgcaataat tttttcctca gagtataagt ttttacttga atatatgaaa aatttaaata
     2041 taaatgaaag aagcttacaa agttattttt catttcaata tgtacttcct gaagatacta
     2101 tgatacaagg aataaggctg ataccagcag gtcactattt tagggtagaa aatggtatac
     2161 tatctttaaa gagatataat aaacttgagt ttagaagtag tacaaaattt ttttatacta
     2221 aaaatcactt aggcaataga gatgtgaatg aggatgatat aagaaatata gtagtagatt
     2281 caatagtaac tcatatggaa gaggagaaag aaattggaac atttttgtct ggtggaatag
     2341 attcatctat tataactact gtggcttcac aaattaatcc aaatataaaa tctttttctg
     2401 caggctttag tgtcaaagga tatagtgaat tagaagtagc aaagaaaaca gctgataagt
     2461 taggtataga aaatatccag attaatataa cacaagatga atatataaaa tcattaccga
     2521 atgtaattta ttctttggat gaccctatag cagacccttc agaagtaggt ctttatttct
     2581 taattaaaga ggcaggtaaa catgttaaag tagcattgtc tggagaagga gcagatgaat
     2641 tatttggagg atataatata tataaagagt atagtactat gaaatcagtt gtcaattcac
     2701 ctacttatat taaaagtata ttaggaagag tttcagaact tatgccaaac ataaaaggaa
     2761 gaaattatct ttatagagct acaacaccat tagaaaaaag atatatagga aatgcaaaag
     2821 tttttgaaaa cagcgaagta aaaagattct tttttaagta taaggaaaag aatatatatg
     2881 aatacctgtt gtctaatttg tatagagatg cacaaaaaaa taattacgat tatataagta
     2941 aaatgcaaca tatagatgtg aatacatggc ttcaaggcga tatacttcaa aaaattagca
     3001 aactttctac agctgagcag gttgaactta gagtaccatt tttatataaa gatgtatttg
     3061 atgtcgcaaa aaatttaaga atggagcaaa aaataaataa gaataataca aaagttttac
     3121 ttagagaggc gtttagagaa atagtcccag aacatgttgt tcaaaggaaa aaattaggat
     3181 ttccaactcc aataagagtt tggttaaaag attctctagg ggatattgta aaggaaacta
     3241 tttataattc aaatgtagat gaatttatag ataaaaaata tgcaataaaa cttttagata
     3301 cgcatcttaa aggtcataga gataattcga gaaaaatatg gactatattt acattttgtc
     3361 tatggcatca attatttatt gagcataaaa atgtcgagta ttaatacttt tttcaatatt
     3421 aatcagaggg caaaagcatg caatgttttc accctcttga ttattatgga ataggataag
     3481 tagaattttg attgattagt ttactatctc aataaaatta ttggcaaata ttttgacaag
     3541 actactttca acagctaaat ttaagttttg tccatcatca tatccagcgg aaatcaatcc
     3601 tagtaagttt ccaaacatat ctaaaagagc tcctcctgaa ctaccactag agattggagc
     3661 tgtaaattgt atcatttgaa ccgtttcaaa atcacgaaaa gctgaaataa ttccatctga
     3721 aactgtatta aaaagaccta gagggcttcc aattgcaact attttttgac ctctcacagg
     3781 ttttttactg actttaacag gtattggctt gcattttcta tcaaccttta tcacagctaa
     3841 atcataatct gaatggtatt ttataatttg gtaagatgta taaactttat catcattttc
     3901 aaatctaact aaaaatgaat acccatcatt gactacatga agattcgtaa gtatataacc
     3961 ttcattattt attactactc cagaaccgct cttaaacggt ttattttcgt tattgcacac
     4021 aacaatcata acaacagaag ctgataactt tgcaaggtct tcataagaaa gttctacttc
     4081 ctcatcttgt acaatttttt tagatgttag atttgtgtta ttaagtgtat ttttagagtt
     4141 aggtttattc gtagtaggag aagtcttatt ggtattttca ctgtaattta tatttgtaat
     4201 atgcctttca aaaggtattt ctaatatatt tggagtcttc ctagaaagtt caaatagagt
     4261 agaaaatttt gaacattcag gatataaatc aagaatatct ttatttggat ttatatataa
     4321 aatatcacac ccaagttgtg ataaaaaata taagaaaaaa agttcttgcc ttttaatatt
     4381 tccaaagtat actatctttg gtgaaatagt agtcttcacc ttgttataaa ataatttagg
     4441 tagaaaatag tctatccaac acaaaagttt aactgcaaag tttttgatga tagaggatgt
     4501 acaatttggc atacttgtgg aaaataaatc taaagtattt ttaaatgcaa tctttttagt
     4561 ccaatttaag gtgtcatttt ctattttaca tggaaagtcg atattatata aatcccaact
     4621 gttaaactta gaactattat aaatattaaa aattttaagc atcttatctg tttgttcttt
     4681 gtcaaaattt atttttagat tgtttaatct aatataggga attgtaggat tattaaaaaa
     4741 ataagtatct atatttttaa ttttttctat gtatttatca attgaatcat caacacctat
     4801 atatcttgta aagtagactt ctttaatata tgaagaactc ttatttttat aggtagtatc
     4861 agatataaat tcaattagag gttcagtaga cttttttatt gaaatcttta atggtttaat
     4921 agtacaaatc atattttttc tcctataata agatgatata atctattata aaacaactta
     4981 aaacaatata gtacaataat tttgtagttt caaaataaaa tatgtacttg tttatataaa
     5041 tgatatataa ttacaataaa attgccgaaa agccctctcc tttaggtgta gggatgaaag
     5101 gtaaggtctt aatataaaaa aagttagaat aatgcttgta atctaatacc gcta
//
LOCUS       DAYASV010000193         5002 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYASV010000193 DAYASV010000000
VERSION     DAYASV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.198"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            62..865
                     /locus_tag="ACGI68_003549"
     CDS             62..865
                     /locus_tag="ACGI68_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139951.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESALKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            877..2007
                     /locus_tag="ACGI68_003550"
     CDS             877..2007
                     /locus_tag="ACGI68_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139952.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            2011..3420
                     /locus_tag="ACGI68_003551"
     CDS             2011..3420
                     /locus_tag="ACGI68_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0139953.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYIIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            3637..4140
                     /locus_tag="ACGI68_003552"
     CDS             3637..4140
                     /locus_tag="ACGI68_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HFH0139954.1"
                     /translation="MFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPIYS
                     NNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRGGK
                     CEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVSSY
                     ISSHIPQ"
BASE COUNT         2092 a          423 c          863 g         1624 t
ORIGIN      
        1 taggaataga ttattataat taatatagat ataaaaataa ttatagaagg aaaggcacat
       61 tatggaaaaa ttgagagtta ttgaaatgat aaaaaataat cccaatatta tagctacaat
      121 agataatcca actgatgaaa tgaaattgtt agctattaaa gaaaatgggc ttgtactaga
      181 atatataaat aatccaacca gagagatgca agaactagca atagataata atattagagc
      241 aattaaattt atagataatc caacagaaga tatgatgata aaagcagtaa atgagggatg
      301 gagtatactg gattatatca aaaacccaac tgataaagtt attgaaatgg ctataaatca
      361 agcaggatgg gcaattaaat atgctaaaaa tccaagtgaa gaattacaac tgttagcagt
      421 aagaaaaaat tatgattcga taaagtttat aaaacaacct tatgaaagtg tacaagagga
      481 ggcagtgaga ataagttatg atgccttgag atatatagat tcacctagtt ataatgcaga
      541 acttatagct ataaaaaata atgaagcagc tattagcttt ataactgatt tagataaaaa
      601 taaaatgttg gaatttttaa aagtgaatat tttagtgatt aagtacattc tgaaaaaagt
      661 atctaaagat gaattagaga gtgcattaaa agaagtatta tccaaagaag atgttgagga
      721 aaagtatatt agagattttt tgaattgtag tgttatagat agaaatagcg aaagtatacc
      781 aatagataaa attatgttta tttataaata tggaagtaag acagcaaaaa aaatagctgt
      841 agatgagaaa ttaaagatta tgtaggagag agaaaaatga attttataga cacattaaaa
      901 agcgttgact taatattatc cactggagaa gtacctttaa tagtaggtga aagtggaata
      961 ggaaaaacag ctttagcaaa taagcttgcc aaagaaaatg attggagttt aattgttatt
     1021 gatggaaact tacttaaaga gggtgaaata ggtggtcttc caactataga atcttatgta
     1081 ggagttaatt ctaatggata taagactgaa aagaaaacaa cagtgtatgc tgttcataac
     1141 aaattaagag aaattgatga agagatatct aaaagtaaga cagttctttt atttatagat
     1201 gagataaatc gttgtgagca tacagtacag caagaactta tgaatttaat tttaaataga
     1261 gaaattaatg gatataaact gcatgatgat gtaaagattt tagcagcaat gaatccatca
     1321 agtaaatatg gttcagattt tgattatcaa gtggtagata tggatgcagc ccaagaaaat
     1381 aggtttgttt ggttaaatat ggagagtgac catacacaat ggataaaatg ggcaatagat
     1441 gagggtattg aaagaaaagt tatcgagttt atttccactt ttccagaata tctgcataaa
     1501 ataaatgaag atgatgtaag agccactcct agaagttatg agagagtatc taaaatttat
     1561 aaagtttata aagaaaaaaa taattctatt cctagagcag tatttttaaa tgttgtaaag
     1621 ggtaatgtag gtaaagttat tgcagaagaa tttataagtt ttattgaatc aaactctgaa
     1681 ccattaatat cctatgaaga tgttttttta ggagaatcta ttgatgagtc tattgtagaa
     1741 agagtaaaaa atgagagtca tacaagactt tatctatcag ctatgaacat tctaaaagac
     1801 ttagaattaa atattaagaa tgataaatat gaatcaaacc actatattaa tagatttatt
     1861 gaatttttaa aaatgtatcc tgtagattta atgataggta ttatgaagga tattagaaac
     1921 agttatattg aagtatataa aaaagctata gaaaatgaag agtttgtaaa atcatatttt
     1981 gaatcttata gtttaataag gggataatgt atggaaaatt attttgataa acaagcaaaa
     2041 gagctttata ataaagcaag tgaaattata aatactcatt ctatgctaaa ggcaaatcgt
     2101 gctaatgaga aatttgatat agatatacca caagatttta agtatgagtt ttttagtctt
     2161 gtagataaag tgaatttaag tcttatggaa gaagaagata atttttatgg atattttttg
     2221 tttcagatgt caagagaaat aagatttgat ataagtagtc ctacaggtgt gaattttaag
     2281 ggagctaaat atattatata ttttaatcca ataatctttt taacgcttga cataaagcaa
     2341 atggagacta caattaaaca tgaaattcat catatattat ctatgcattt gatgagagca
     2401 aaggagttaa aaggaaagta tagtacatta gcaattaata tggcaatgga tatagtagta
     2461 aataaatttc taaacaattt accaccatat gcaactacct tagagtgggt aaatttaaaa
     2521 tattccttaa aacttgaacc atatgaacct tttgaatatt atgtagaaaa gattcagact
     2581 gaattagatt tgatggaagt ggatgaagat ggtgaggaag atgatagtga taaatatgga
     2641 gatatagaaa ttgattatag ccccgaaaga actcatgata tatgggatga atccaatgag
     2701 atagatgaaa atacccttag agaatttact gaaaaattta ttaacagttc tcaaaaaggt
     2761 aaagttcctg cttatttaga aagtatgata tcatcactta aaaatagtaa gggtgaattg
     2821 ccatggaatt tatatcttaa tagaataatg ggaacagttg aaaataataa aaagaagact
     2881 ataacaagaa gaaatagaag acaaccaaat cgtttagatt tgaggggaga acttagaagt
     2941 cataagtcag aaatagcagt tgcacttgat gtaagtggaa gtattagtga tgaagagttt
     3001 aaacaagcta ttaaagaagt tcttagtata gtgaaaaact ataatcatga aattacaatt
     3061 atagaatgtg ataatgaaat tagacgtgta tataaagtga gaactgaaaa agatataaaa
     3121 gatagaatta ggataggtgg aggtactaga ttttctccag tctttgagta tgctaataat
     3181 aaaaagatta atttgttagt ctactttact gatggtaaag gtgaaaataa actacaagta
     3241 atacctagag ggtacaaaat tttatgggtt atttctggaa gaggagataa aatttcatta
     3301 aaagaacctt atggagcagt taaaaaactt actaaggtta aaacaaatga tgaaatatta
     3361 gaaataaatg atgtaagaaa tgatgggtac tcaatgaata gccaagcacc aattttatag
     3421 gtattcacag gagataagct gtattaattt attttgatat gcttgttata aaaatctaaa
     3481 aaatacatta atcaatggtt aatgtatttt ttaattttaa taatataagt tttacttaca
     3541 tttaagtctc aaaatgtgtt aaaatataat atatagcaga tagttttggc aaggtggatt
     3601 tagttgtcaa aactattttt tttattaagg atggtgatgt ttttgtttaa gattgatgat
     3661 tatataatgt atggtatgac aggagtctgc aaagtcatgg atataacaaa tgaacgattt
     3721 acaaatggaa taaaaaaaga gtattatgta ttgagtccta tttattctaa taatacagta
     3781 ataaaaattc ctgtagataa tgaaaaagta ccaatgagaa agttactttc taaaattaat
     3841 gtgctttcat taataaatga tattcctaat atggatacat catggataga taatgaaaaa
     3901 ttgagaagtg agcaatttaa aaaaatactc agaggtggta aatgtgaaga gttaataaaa
     3961 ttagtaagaa gtatagataa taacagagag tatgtaaaat ctattggtaa aaaaacacat
     4021 caagcagatg ataatataat gaaagaagct gaaaggttat taaatgaaga atttgcaact
     4081 attttagata tttctcctaa tgaagttagt tcatatattt caagtcatat acctcaataa
     4141 agtgttttag tatcctttat ttaatctatt ttaagtatta tttatgtatg ctggttttgg
     4201 taaaataaaa tatataatta aattatataa aaaataataa tgattgtatt gaagaattac
     4261 agaatagtgc ggagagccta ctcctttagg tgtgagatgg atatcactta aagatttaaa
     4321 ttgttattgt agatgttttt aatataaaac aagattaata agtgtattgt aagctttaag
     4381 tgaaattgat actataatga gttacttaga ttttaaaata tataatatta taaaaaatcc
     4441 ttttaggttt tgaatgaata agatggatac tatgttgaat agaatccatc ttatttaagt
     4501 tttatttatg ttgtttattt gtaagcatta tttttgtaca tgaaaacaca gtgtaaaaat
     4561 atgcttagaa ttatgaagtt ttattaaaaa gatattcgac aaaataagac taaaaatatt
     4621 cttaaactta taactaagat aaatattgta tatttatagt gtagcctgaa aaattgtgga
     4681 tgtacgaaat ttatttagta ttttattgaa acaaagtaga agaagtacta tagtttatct
     4741 ttgaatagat tgttattttt tagtgaaaaa gttattaggt taatacataa atataatagc
     4801 ttataaatag tttaagcatg ttaaaaaagt agttattaga gatatatagt tacattgctt
     4861 ataagatact taaaatgata gcattcactt atattcagaa gattaagata aaaaatcatt
     4921 tttatatagt aaagcatacc taaatttaga aaataattga atttataaaa ttaattaaat
     4981 acataaaaaa gtattcaatc aa
//
LOCUS       DAYASV010000194         4967 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYASV010000194 DAYASV010000000
VERSION     DAYASV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.199"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(563..1204)
                     /locus_tag="ACGI68_003553"
     CDS             complement(563..1204)
                     /locus_tag="ACGI68_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HFH0139955.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1447..2250)
                     /locus_tag="ACGI68_003554"
     CDS             complement(1447..2250)
                     /locus_tag="ACGI68_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0139956.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFAQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2406..3581)
                     /gene="thiI"
                     /locus_tag="ACGI68_003555"
     CDS             complement(2406..3581)
                     /gene="thiI"
                     /locus_tag="ACGI68_003555"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFH0139957.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEESTILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3627..4778)
                     /locus_tag="ACGI68_003556"
     CDS             complement(3627..4778)
                     /locus_tag="ACGI68_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFH0139958.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVDNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1548 a          936 c          474 g         2009 t
ORIGIN      
        1 aaaaagttac tatttattga aaatttagat atttttctaa aaattatcta tgttaatagt
       61 taaatttatg aacatttttt gtttttatgt taggatattt cttagaaatg aaatacttct
      121 taatttgatt tgatatgtat atttttatag ctattttatt ataaaaaaaa agaacccttt
      181 ttataactaa gggttacttt ttttctctat tttttcttat tatatcacta ttttttccct
      241 atttcaatat tttgacattt tttccattat actgaaaaga tatcttaaat ttttaacttg
      301 gataaatttt aaagcatata cttaaaatta acatacttgt tttctatttt attacactta
      361 atccttattt ttatcattct acaaaacaaa aataaaaaag aataagataa taaataaaat
      421 acttatcacc ttattcttta tttaattatt ttacaacgaa aaatctaaac tactttttaa
      481 atagcttcct cttccatcaa tttatttgta tattactgat ttaattcttc aagtaactga
      541 ttggttcttg caatacttta tcctaaactc tggaaacttt gtaaactacc tctctagcat
      601 ttggtaaatt tccatctgga atacctacag caataccaaa tggtacagaa tacccttctg
      661 gtatatcaag tagttctctg tattttttat ttgtatcaaa cttcatcttt attgactctg
      721 attcttttac atcttcttta tccaatacac tcacaagacc ttcaatatcc ccttgttcaa
      781 caggtgtaaa taaacctcta accattccta ttatacaact tccaagccct agtgcttgtg
      841 cagctgttgt tatattccct gtaagtattc ctatatcata tggagcccat gaactacttt
      901 catcataaga aataaataaa acggttggag caccataaaa taacttaaag tttttattta
      961 tctcaacatt actcacctca tgaatcctag ataaagtatc atcactaatc tcttttaaaa
     1021 gtttctggtc ttgcacaaca gttatgtgcc aaggctgttt attacatcct gaaggtgctg
     1081 caaatgcaca gtgtaataat gtgtttactt gctcatcact aatttgttct gtcgtaaatt
     1141 ttcgtatact tctacgactt tgtattaaat ttattgtttg gttgtcttga aaattattat
     1201 tcatatctat tccccttttt atgtatttct tagtacaaat actaaatctt cagaaaatta
     1261 atcttataat taaatttctt tacaatatta ttataattat ttttagcttg tatgtgaaga
     1321 cttaaaaata taaacacttt taatatatct caattatata tataaatgaa aatctggtat
     1381 tcgtcatcta tagaaactgc tcttttctat agtttaatca ataccagatt aataaaacat
     1441 gatatattaa tttaaaggac cccatccctc tttagaatga atctctgttt ttacagactt
     1501 aacactatct gtgtcaacat cccatgacct acctgcaaca gaaataccat ttttaccttt
     1561 ataccatcta ccttcatcat aatcgtaaac tagggaagta taccaattac ccccagtctt
     1621 attatttgca aatacaacta cagtagcatc agatttagta ggattatatg catatggttg
     1681 tatatatagt gtatccttac ttaatccata ttttgcctta aattcatctg gaattgtagg
     1741 ccacccattt ttatatttat tgtctaataa atattttcta aaatcatcat taccctttga
     1801 ggctcctggt attatagaag ggtcttgtgc aaatgatgca atcaaatctt tcatactttg
     1861 atgtatatcc ctagccattt caacaccatc tggatgtttt gtacaagtaa catacacttt
     1921 tgcaatgctt tctattccag ttattccatc ataaccatca tcaaacgttg ctgaatatat
     1981 tccacctgac ttacactttg gttctgtttc aaagtactta ccatctttgt tttctaatac
     2041 ctctttcatg acttcattct tgcctttact tgattcctca gccattgcaa taacaatttg
     2101 agtctttata ttttctctat tagaaagaca tgtaactgct ttactttttt ctatatttct
     2161 aaacaatgct ggaacagcta ctataactaa aatgcctatt atagatataa ctactaacaa
     2221 ttcaattagt gtaaaaccct ttttattcat aataatcatt cccattaaat aaaattatag
     2281 tacttatttt taaatactac atattaataa attgtctaca taaatccttt caacagaact
     2341 actaatatac ttacagaact actaatatac tcaatattca atttagtaat taaataactt
     2401 gtatgctaaa actcaatatc ctctacctct attttgtcaa tagcatcctg tactaatttt
     2461 tcaatgtcaa gtgctgtttc agatttttct attttctcta atctaggttt agtgacgggc
     2521 tttctaggag aaaatacact acaacaatct tcttcaggca ctatagatgt ttcaaaagtc
     2581 cctatttttt tggctatatc aactatatct gatttatcca ttgctataag tggtctaaac
     2641 actggtaagt ctacaacggc attagtacaa gtaagtcctt gtatagtttg agatgcaacc
     2701 tgcccaatac tttcacctgt aattaaagca tcacaatgtc ttttttcaga aagtctctgc
     2761 gctatcctca tcataaatct tcttgaaaga atagtagatt cttcttcatt acaattctcg
     2821 ccaattgctt tttgtatttc taagatatta actttatgaa gccttactct accacagtat
     2881 tttgccagta ttttagctaa atccttaact ttttcctgtg acctttcact tgtaaatgga
     2941 taactatgaa agtgaactgc ctctacttcc atacctcttt ttgcaaccat ccatgtagct
     3001 actgggctat ctatacctcc tgataaaaga gacatagctc taccattcgt tccaagtggc
     3061 aatccaccat atccaggtat agtatcacta tagaccatag tatgaaattc tctgtattcg
     3121 cactttattt taacttctgg atttcttaca tcaacattaa tcctatctcc tacctttgat
     3181 aataagtatc cacctatatc catactcatt tcttgagaag tcaatccgaa agacttatct
     3241 cctcttcttg actctacctt aaaagtttta tatccagcct ctattttttc ttctaacatt
     3301 tttaaagcta tttcttttaa tgtatcataa tcttttttag ctcttactcc agggcacaca
     3361 cccactatcc caaatacttt tctaacttct tcaattactt cttcataatc atagtcttct
     3421 aaatcaacat atattcttcc atactcttta tatacattaa atcttccaat tggttttaac
     3481 atatttctta tatttcttat aagcctattc tcaaatatat atctattctt tccttttact
     3541 ccaatttctc catactttac tattaatata ttatacacca tttcacctct taaactaatt
     3601 tattatcact atttatacag tattctttat tttcttttca ttattgacct taagtcacaa
     3661 attgattctt tcaaaacttt cacagcttct aacatttctt cttctgtatt catatcagat
     3721 aaactaaatc ttattgttcc atttatttct tcatttgtaa gacctatagc atttaatacg
     3781 taactacctt tctttttaga agaacatgca gagccagttg atacataaat acctttttgc
     3841 tctaagtaat gaagtaaaac ttctcctttt gtccctctaa atgatacatt taatatatga
     3901 catactccgt cttctggaga attgattttt atatcttcta tattatcaat tatttcattt
     3961 tttaatatat tttttaagtt atctactttc gaaataacag catctaaatc tttgtttagt
     4021 attctaacag cttctccaag accatatatt ccaggtacat tttctgttcc cgacctaatt
     4081 ccaatttcct gaccaccacc tgttaacata ggcttaaggc gattattttc ttttatatac
     4141 ataaatccaa ttccctttgg accatgaaat ttatgtgcac ttacactcat aaaatctata
     4201 ttgtatttag atggtctaaa gttaattttt ccatatgact gaatagcatc aacatggaaa
     4261 taaatttttt cttttaaacc cttcaaatac tttccaactt cacctattgg ctgtatagtt
     4321 cctatttcat tgttaacatg catcatgctt actaagcatg tagttgactt tatcgcattt
     4381 tttaaatcct ctatatttat tttcccatct tttccgacct ctaaataagt tacttcaaaa
     4441 ccatcttctt ctaaatcttt taatgtattt aaaacagaag gatgttctat gttagtagat
     4501 attatgtgat tttttctttt tttatttaaa ctagtaatac ctcttattat agtattatta
     4561 cactctgtac cacctgatgt aaaataaatc tccttttctt tcgctcctag agatttagct
     4621 atatcttgtc ttacttcttt tatagctttt tcaacttcta caccttttct atggacagaa
     4681 gaaggatttc catattccgt attaagtgca taaaccattt tatctataac ttcttgataa
     4741 ggttttgtag ttgcgctatt gtctaagtat atttccaact taattacacc tacctttatt
     4801 taaaatcttc aactctaact attataccac taattatagt attttttttg ttaatcttta
     4861 ttaaaaagac aatcttaaat tataaaatat tatttcatga aaagctagtt ttttcacttt
     4921 attacaaatt tttatagtaa aatataatat acttatacta tacccta
//
LOCUS       DAYASV010000195         4931 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYASV010000195 DAYASV010000000
VERSION     DAYASV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.200"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            630..4772
                     /locus_tag="ACGI68_003557"
     CDS             630..4772
                     /locus_tag="ACGI68_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HFH0139959.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISSVTSPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFS
                     EGESAIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTN
                     VFFRDLIPNGLEFIPGTVTVDGVSVPGVDPNTGFPLPDIGGGTGVEVTFDVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPASIVNPEYSLDGGVTFQPWTGSLNIGTLEVGETR
                     VIIIRGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGT
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVADEDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWT
                     GSLNIGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSA
                     DIEVIKTSNMDTAVPGETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVT
                     FQPWNGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSTTPDPNPDNNTSTDE
                     TDISPLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYS
                     IDNGVTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNN
                     NISTSEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPAS
                     ILSPEYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTT
                     PDPNPDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNI
                     AEITSTTPDSNLNNNISRVQVEIKQCCCNDCCCNNCSNCCCNDCGCKDCCCNCCKNDF
                     WC"
BASE COUNT         1899 a          726 c          833 g         1473 t
ORIGIN      
        1 caactaaaat tattgatttt agattaaagt accggattaa gcatttaagt agattttaag
       61 cttgatttga ttagaaaggt acaaactatc accgaaaaaa attaaaccca tttaaaaagc
      121 aatataaaag cttgttattt ttgagaaaaa tagaccctct aaactcaatt agattaaaaa
      181 taaaatacag aaaattatat ctctaaatat cttaaataat taagtttaaa ttttacgata
      241 ttttgtttga tgatttataa tataaaagca tactcttaat cttcaaaatt taaaaagagt
      301 aaaaaataac aaaaaaatca ctgacatttc actgacaaat tgtaattttg ttattataaa
      361 tacattgaaa atacttgatt attgaaaaat ggcataaaat aatacatttt atgccatttg
      421 ctattatttg atagttttta acttatgaca taagatattt tttaacatat acaataaaat
      481 ataaaaaaat tgatattgca aataaaaaaa aatacatatt agatgttata gaaaaaaagc
      541 gcacttatag gcttaaaatg tcacataaaa gagttttaaa gaataatata ttatgaacac
      601 aaattttgta atcacaaagg agtgataata tggcaacagt atcaggacaa atagtttttg
      661 atagaaatag aagtgctacc attgattctg gagactcagg aatagccaat ataccagttg
      721 tattacaaga tatagcaact ggagttagac ttgtggttct cacagatgca tcaggaaatt
      781 atgtttttac taatgttcca aatggtaact atagaattgt agaagctttt ggaactacag
      841 gaggagtacc aacaccagga aattttgcaa cagcgatagt aggtccagtt ccaattgcag
      901 aaatgccacc tattagttct gtgacaagtc ctcctattgg ttcaacaaac ttagatggta
      961 caacacctaa cacactatta ataacagtag caggttcaga tataacaaat caaaatatat
     1021 taaatgggcc tgtgatttat acacctatag agaatatatt ggattcttgc gtatctgtat
     1081 ctaatataaa ccttattaca gatgcagata acggaacatt cggttttttt ccaccaggta
     1141 caccaagtaa tacaggacct gaaatagaac cttacccaaa tgtgacgcca gattttacgt
     1201 atgttgtacc agacccaact aagtttacac ctttagatgg agaatataca gtacaaaata
     1261 ttatgacaaa tgcaatgagt aatgttatag gagcttggtg gcgtattgct gaccatacta
     1321 caggaaatga aacaggaaga atgatggtgg taaatggttt taatcctgga gctgtatttt
     1381 ttaaatcaac agtacaagtt acacctaaca ctaattattt atttagcact tggattttaa
     1441 acctttttaa ggtaacagga tatccaccac cacagctagg tgtaagaata tttgaccaaa
     1501 ataataatat actatatcaa gcaacattag gagctcaaat tccagttaat acaaacgcac
     1561 cagaatggaa agaagtaggt actgtgatta attctcaaaa taataatcaa cttacagtag
     1621 aattttttag tgaaggagaa agcgccattg gaaatgacta tgctatagat gatgtttctt
     1681 taagagaaat agctgttcca acttttactc caataaaaac aagtaacaca gaaactgcta
     1741 atgtaggtga tataatcaca tatacagtta cattagataa tacttgtgat agccctttaa
     1801 ctaatgtatt ttttagggat ttaattccaa atggtttaga gtttatacct ggaactgtaa
     1861 cggttgatgg agtatctgta ccaggagtag accccaatac aggtttccca cttccagata
     1921 ttggaggagg aacaggggtt gaagtaactt ttgatgttgt ggttgaaagt attcctaacc
     1981 caaatcctac taataatata gctactatag attactccta tacacctgta gaaggcggta
     2041 taccaaatga tttttcagta gattctaatc cagttcctgt tgaagtaatc tcagcagata
     2101 ttgaagttac aaaattatct gaacctacta tagtgaatcc aggagaggaa ttaatataca
     2161 ctataaaagt agtaaataat ggaccattcc catctgaaaa tgtggtttta acagatgatg
     2221 taccagcttc tattgtaaat ccagagtatt cattagatgg aggagtaact tttcaaccat
     2281 ggactggaag tttaaatata gggacattag aagtaggaga gacaagagtt attataatta
     2341 ggggtattgt aaatccatca acagtaggaa ttataacaaa tacagctgta gtatcttcaa
     2401 ctacagcaga cccaaacctt aataataata catctacaat agagacagaa gtgaatttat
     2461 tagcagatat tttagtaatg aaaacagcag aacctaattc agctgttcca ggtactttat
     2521 tgagatatac tattcaagta gaaaacttag gaccagcaaa tgctgaaaat gtaatattaa
     2581 atgatgatat tccagcttct attataaatc cagaatattc attggatgga ggagcaagtt
     2641 ttcaaccatg gaatggaagt cttaatatag gaactttaaa ctctggaatt tcattaacag
     2701 ttttaattca aggaacagtt agtttaaatt cttcagaata tatagtaaat acagcaacag
     2761 tttcttcaac tacacctgac ccagatttaa gcaacaatat atctaccatt attacaccag
     2821 ttaacccaca agcaggtatt tctattataa aagtagctga cgaggatgta gctgttccag
     2881 gagaagaatt tgtatataca attgaaattt ttaatgaagg accatctaat gctacgaatg
     2941 tagttttaac agatgatata ccagatgtta ttttaaatcc agaatattca ttggatggag
     3001 gagcaacttt ccaaccatgg actggaagtc ttaatatagg tacagttgca ccagggcaat
     3061 taataagaat tatcataaag ggattagtaa gttcaactgc tacaggagat attactaata
     3121 cagcagaagt aagtgcagat gtaccagaac cagttacgga ttcatcaaca gtgacaactc
     3181 ctatagttcc atcagcagat atagaagtaa taaaaactag caatatggat acagcagttc
     3241 caggagaaac tttcagttat actataacag ttacaaattt aggaccatct gctgcacaaa
     3301 gtatcgtttt gacagatgat ataccagatg ttattttaaa tccagagtat tcattggatg
     3361 gaggagtaac tttccaacca tggaatggaa atcttagtat aggcactttg gatgctggag
     3421 aaataagaag tattataata agaggaacag tgagccagac agcagtaggt acaattataa
     3481 atacagcaac aataagttca actacacctg acccaaatcc agataataat acttcaacag
     3541 atgaaacaga tataagtcca ttagcagata tttctgtgat aaaaggcaac gaaccagttg
     3601 ctataccagg gggaagattt atttatggaa ttgaaattgc aaatgcagga ccatcttttg
     3661 cagagaacgt aactttaaca gataatatac cagcttctat tttaaatcca gagtactcaa
     3721 tagataatgg agtgactttc caaccatgga atggaagcct taatataggt actttagatg
     3781 ctggagagat aagaaatatc ataataaggg gaacagtaag tcaaacagca ataggcacaa
     3841 ttataaatac agcaacagta agttcaacta cacctgaccc aaatctaaat aacaatattt
     3901 caacatcaga agcagaggta agttcatcag cagatatttc tgtagtaaaa cgaagcaatc
     3961 agactgttgt tgtaccagga gatgtactag actatacaat tgaggtgaga aatgcaggac
     4021 cgtctactgc tcagaacgta actttaacag ataatatacc agcttctatt ttaagtccag
     4081 aatattcaat agataatgga gtgacttttc aaccatggaa tggaagtctt agtataggca
     4141 ctttagatgc tggagagata agaaatatta taataagggg aatagtaagt caaacagcaa
     4201 taggtacaat tataaataca gcaacagtaa gttcaactac acctgaccca aatccagata
     4261 ataatacttc aacatcagaa atagatataa gttcatcagc agatatttct gtaataaaat
     4321 tagcaaataa aacagaagca tgtgtaggag agcaaataga ttttgtaata gtagtatcaa
     4381 atgcaggacc agaaaatgct caaaatgtta ctttaataga caatgtttca gataagctta
     4441 agaaagcagt attttcactt gatagaggtg taagtttcca accatggact ggaagtctta
     4501 atataggtac actaccagct ggaactttaa gagtgattat attaagagga attataaaat
     4561 caacatgtct tgatagatta acaaatattg cagaaattac ttcaacaaca ccagattcaa
     4621 accttaacaa taatatatca agagtgcaag tagaaataaa acaatgttgt tgtaatgatt
     4681 gctgttgtaa caactgtagt aattgttgtt gcaatgattg tggttgtaaa gattgttgct
     4741 gtaattgttg taaaaatgac ttttggtgtt gattttataa aataattttg tataccttaa
     4801 attaaactga aataaaaaat aaattcacat atgagtaaaa tattttataa atattaataa
     4861 attttctatc tttgatatgg accacattcg taatactagt tattaaatga agtggtcctt
     4921 tttaattcat t
//
LOCUS       DAYASV010000196         4896 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYASV010000196 DAYASV010000000
VERSION     DAYASV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.201"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(370..1173)
                     /gene="proC"
                     /locus_tag="ACGI68_003558"
     CDS             complement(370..1173)
                     /gene="proC"
                     /locus_tag="ACGI68_003558"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFH0139960.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEKFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1498..3867)
                     /gene="hypD"
                     /locus_tag="ACGI68_003559"
     CDS             complement(1498..3867)
                     /gene="hypD"
                     /locus_tag="ACGI68_003559"
                     /EC_number="4.2.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase"
                     /protein_id="HFH0139961.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3872..4780)
                     /locus_tag="ACGI68_003560"
     CDS             complement(3872..4780)
                     /locus_tag="ACGI68_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019471 - 4-hydroxyproline metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-4-hydroxy-L-proline dehydratase activase"
                     /protein_id="HFH0139962.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1474 a          927 c          523 g         1972 t
ORIGIN      
        1 taaaaagtaa aacattatag gtattttaaa acttattagc gaagaaaata tttgtaactt
       61 ttattacctt atgaatatta tttcaaattt aaaaaggcta taaataaatt tatttttaaa
      121 taaatctact tataacctca caataatact tataaatttt attttttcac aatgatatat
      181 ctcaaatata gttaaaaata tatctattta attattaata tttaagtaat aaattatcac
      241 tcaatcatta aattcctttt tatagtaaat tactcttatt ttacttatac caaatacatt
      301 tatgtataga tataaataag cactaaaaaa acaattcaag aaaccatata gtaaacttaa
      361 ttacatctgt tatttgctca tatctttaga cttatctata caagcaacaa tagattttat
      421 aactgctgct ctaaagcctt cttcttctaa taccttaaca gcttctatag tagttcctgc
      481 tggagaacaa accatatctt ttaattcacc tggatgtttt ccagtttcaa gaaccatctt
      541 agctgacccc attactgctt gagatgcaaa tttataagct tgttgtcttg gcattccagc
      601 taatactgct gcatctgcca tagcttctat aaacataaat acatatgcag gagaagaacc
      661 actaatacct gtaactgtat ccattaaata ttctgatact atttctgcct caccaaaaga
      721 ttcaaacaga gccttaatca tatttaactc ttcatctgtt acattactat ttttacaaag
      781 agctgacatt ccttcgccaa caagggctgg agtattaggc attactctta ctattttttt
      841 atctgaacct ataaaacttt ccagactttc tatagtttta cctgctgcta tagatactat
      901 tattttattt gcatctatta attctttaag tggagttaaa gccttttcgc atatattagg
      961 tttaagtgct actattacca catcagaatt tgtaacagct acctttgaat cagttgtagt
     1021 atttactcca aatttttctt ttactttatc taaggcaact tggcttaaat ctgatacagt
     1081 tatatcttct gcctttacta atcctgactg tactatgcca cctatcattg cacttcccat
     1141 attccctgaa ccaataaatc ctaaagtttt catatcaaat ctcctttttt ctattagttt
     1201 tattttaaag tagctaaatt gactttattt taactgctta aaattatctt atttttataa
     1261 aatataacct tattataact ataataaatt attcttttgt atgtattact tactaatatg
     1321 tgtatgattt actaaatata aatttaacga ttttttcatt aaacttaacg agctaacttc
     1381 ctatttagaa agttagctca taattaatct aaaattttaa aaatggaatc aaaccattta
     1441 tactttatta tatcagttac gattttatat agtaagaagt tttatttaca agcaaaccta
     1501 gaatgtttgc tcagttcttc ctattatctc atcttgtagt gttctactta aattattgaa
     1561 atggtcacta tatcctgcaa ctctaactat taagtcttta taatcctgag gattcttttg
     1621 agcctctaac agtacatttt tatcaaatac attaaattgt atatgatgac catccatatt
     1681 aaagtatgct cttactagat tagccatatt atctaaccct ttttctcctt gaactactga
     1741 tggtgcaaat ctttgattta ataatgttcc accagttttt atgtggtcca tcttagcaca
     1801 agattttata actgctgtag gtccattagt atctccacct ttttcaggtg aaattccctc
     1861 tgaaactggt tttgcacaaa gtctaccatt tggacttgct cccataattt ctccaaagta
     1921 tatatgacaa gttgttggta acatatctac tctgtattct ccaccacaaa cagttggtct
     1981 accagttacc tcattgtaat atgcattgaa tatctcttgc attatttcat cagcataatc
     2041 atcatcattt ccatatttag gagtcttatt tgaaactaaa ttaagtatag cttcatatcc
     2101 ttcaaaatta gcatcaagtg ctttaagcaa tgtatccata tcgaattttt tcttatcaaa
     2161 tacattatat tttattgctg ttaaactatc tgttatagtt cctataccaa caccttgtat
     2221 atatctagtg ttgtatcttg ctccacctct ttggtaatcc ttgccttttt ctatacaatc
     2281 atcaactatt gtagacataa gtggacaagg catgtgtttt gcacatatat tttctatcac
     2341 agcatttcct tctattttta tgtcaatcat ataatgtaat tgctttttaa atgcttcaaa
     2401 tagttcctca taagattgga aatttctagg gtctccagtt tcaataccta tttgtttttt
     2461 actaattggg tcatatccat tatttaatgt taattcaagt atttttggaa tgttcatata
     2521 tccagttaga acatatgctt ctttaccaaa acatccagtt tcaacacaac cactggttcc
     2581 accaagtctt gcatcttcta tagtttttcc agcttccata agctcttgaa ctattgcttc
     2641 agtattataa aatgctggtt gaccccatcc ttttcttgat atttcacaag ctctctttaa
     2701 gaatttttga ggagtctttt tacttatttg aacatttgaa ctaggttgta tcaatttcat
     2761 ttcatccata acatcaagta ttatataact aacttcatta acaccatctt gaccatctgg
     2821 atttattcca ccagtgttta tatttgcaaa atctgtatat gtgctacttt cttttaatgt
     2881 tataccaact tttggtggtg ctggctgatt gttaaattta acccataaac attcaagtaa
     2941 ttcttgtgct ctatctctat ctagtattcc attttctaca tctctttcat agaaaggatt
     3001 tagatgttgg tcaagtcttc ctggagtaaa agcatcccat atattaagtt cagtagtaac
     3061 ccctatatga acaaaccaat acatttgtat agcttgatga taagtttctg gtttgtgagc
     3121 tggtacaaca tcacaagttt cagcaattag taataaatct tttttcttag cttcatctgt
     3181 ttcttcttct gccatttgtc ttgctttctc agcatatctt ttacctaata taaccattgc
     3241 atcacaagct attgccatag cttctaaatc tgctttttta ttatatgcat ctaaatcatt
     3301 aagaaaatct agctctttta atcttgcttc tatgtctttc tttaaatcta aaaatccttt
     3361 tttatatatt gtatctccac aaactgtatg tcctggagct ctttgttcca taaactctgt
     3421 aaacatacct gcttcatatg ctgctaacca ctcaggtgtc attgcattta ttatcttatc
     3481 tcttgtttgt ctctttttcc aaaaaggaat tatttcttct ttatgtattt ttctagcttc
     3541 ttctgatacg ctaaaattta ttatatctct attgtgcatt acttctaaat cttccattgt
     3601 atgacaacaa atttctggat atgttggtgc tccattagga gaatcacctt tttcacctac
     3661 tattaattct ccatcattta tgctaagagt tctattctca atataatgct taaatgataa
     3721 agcacgaagt acaggaatct ctacacttcc ctcatatttt ttataagctt ctgtcataag
     3781 tactgctctt tcaatagata tgtgtggttc tgcattaata ctttcttctc ttaatttttt
     3841 agttctctca aaagttcctc ttgccatttt attaccctcc aatcttagta ttgaaattac
     3901 ttgcttcaaa cagtcttttt atttcctcta atttttcttc tgatggtctt tgtagttctt
     3961 ctccttcata ctttttataa attttatcat acttgtgttt accaatatta tgatatggaa
     4021 gaagacttac tgcttgtata ttcaaaggct ttaaaaactc tatcattttt ttaacctcta
     4081 aattttcatc atctacattt actcctacaa ttaatggtat tcttatattt atgttagccc
     4141 ctaattcact taaaatctta acatttttca gtattaagtc atttgatttt cctgtaaatt
     4201 ttatgtgctt atcttcatca ataagcttaa tatcatataa aaacaaatct gcacatttag
     4261 caactctttc ataattttct gactttgcat agccacatgt atcaattgct acatggattc
     4321 cctttgattt gcacatgttt attactccac aaatgaagtc catatcttga gccattactt
     4381 ctccacctga tagagttact ccaccacctg attcttcata gaaaattcta tccttctcta
     4441 tctctttaac caaatctctt acagaatact cttgaccaac aatctctctt gcattgttta
     4501 cacaatagtc taagcaagtt tcacaaaact cacacttatc ttggtctaag catatttttg
     4561 tttcaccttt gtaaattgcc ttatgagggc atatatttat acatgcttca cattttgaac
     4621 atctttcttc attatacaaa acttgttttg tatatgtttg actctctgga ttatgacacc
     4681 aaacacattc aagtggacac cctttaaaaa atactgtact tcttatccct ggtccatcat
     4741 gtatgctaca tttttgtaag tttataacta atggattcat gatttttcac ccctaaataa
     4801 tatccattcc attttttcat ttttatgcaa ttcatcttca tagaatatac ctatgaaaag
     4861 catttctaca attacataat actatattcg ttatta
//
LOCUS       DAYASV010000197         4890 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYASV010000197 DAYASV010000000
VERSION     DAYASV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.202"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            249..1421
                     /locus_tag="ACGI68_003561"
     CDS             249..1421
                     /locus_tag="ACGI68_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0139963.1"
                     /translation="MIIWLSDPINILFTLFGYFILLIICSKSKLIPIISVVMILYPII
                     IGLNFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQY
                     LSNKIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAIL
                     ANIGVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIES
                     YLEIEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNID
                     IDNHLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKV
                     NPINRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTESEFSLFLYIRLF"
     gene            complement(1757..2326)
                     /locus_tag="ACGI68_003562"
     CDS             complement(1757..2326)
                     /locus_tag="ACGI68_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HFH0139964.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVEGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYSNRYN"
     gene            2729..3301
                     /locus_tag="ACGI68_003563"
     CDS             2729..3301
                     /locus_tag="ACGI68_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HFH0139965.1"
                     /translation="MEYIINPCSGIKIKITRGQKIEIVDIEGGQVVDFFAEMKDDSTE
                     FISPGVTIDCNESLRLQVGNLIYSNKYRPMFKILYDDVREHDLLHPCCRPEMYDFFYH
                     NGENHPNCFDNINRTLGTSHQIIHPINLFMYTKVNTDGSIEVCEPLSKANDKIILEAL
                     ENMTLGIAACSVSESSCNSRKCTSIKILVD"
     gene            complement(3980..>4890)
                     /locus_tag="ACGI68_003564"
     CDS             complement(3980..>4890)
                     /locus_tag="ACGI68_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0139966.1"
                     /translation="GNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIG
                     TFGKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHKYIFGMVVLIFVIRFIFSAVSF
                     GSGAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPL
                     TGIILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKER
                     GEKILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVT
                     MTDERDSGAVYDKMENLCKEHTAHIF"
BASE COUNT         1770 a          806 c          573 g         1741 t
ORIGIN      
        1 ctaatttcca gaattttaat ttttaagatt taatttttta aaacttagtt tccagaatct
       61 taattaatag aaaggtatat taaatgggca cttattcttt aatctctaca agcataaatt
      121 ttattctaat attaatcaat attttcatag ccttttttct ttataagttg acttcctatt
      181 ttatagactt taggaatcat tggattttca aaatcatatt gattttgggc tactctatta
      241 ttagtcaaat gataatatgg ctttccgacc ccataaatat tttgtttaca ctattcggat
      301 attttatact acttataata tgtagcaaaa gcaaattaat tcctataata tcagttgtca
      361 tgatacttta tcctattata attgggctta attttctttt tataaacaac cctatatatg
      421 aggaacttat acactcaaaa gagtatattt taatactttc aatagtccag ataatttctg
      481 ctctaattaa attattattt tggtttttaa tacatatttt cctaaagaat agattggtca
      541 atataaaaca atacttatca aataaaatat ggatttttat tgatatggta tgtatatctt
      601 catttttatc aatattaatt gctattattc tgccacctac taatttaatc cctatgggta
      661 gtaataagaa tattatctta ggcaacttac cttatggcac ttatctaatc atcttgtcag
      721 caattctggc aaatatcggt gtgatacttc ttttacagcc attgattgaa aatgtaaaga
      781 tgaagataga aaaacaagca aatcacctaa aagaaaaata ctataactta ctagaaaatc
      841 aacaaattca aatacgcaaa atgcgacatg atatgaataa tcactttcaa atgattgaat
      901 cgtatttaga aattgaagat attgctggag ctaaaaagta ttttaatcag ttaaaaatag
      961 gtataggtac tcttagtgga aaacaatttt gcaaaaacca agctcttaac tcaattttaa
     1021 atcttcgtca tacaagttta gaagaaaatg gtgttgatgt gcactttaat atagatatag
     1081 acaatcactt gggtatagaa cctatagatt tatgtactat tttttcaaat tctttagata
     1141 acgctataga agcatctttg aaaattcctg atttgtcaaa tagaaaagtg gtattaaagg
     1201 cacgttgtga gaaggagtat tttagctatt tattgacaaa caacaaggta aatcctatca
     1261 atagaaaaca agaactattt atttcagata aaaaagatag taattctcac ggatatggta
     1321 ttgagaatat tagagaaatt gttaataagt acaatggaaa attggatatt tcttatactg
     1381 agagtgaatt ttctttattt ctttatatta gattatttta gtctaaaatt agattatctt
     1441 atataataca aattagattg tttgatacta tgtaaaatta taataactta taattaaata
     1501 tttacacata tattcattaa cttaaaattt aatcaaatgt gatattttat ttaccttagt
     1561 aaaaatgtgt gtgtctcaaa ataaactttt tagttgatga ggcactcttt tttacatgaa
     1621 atcaaatata tttttatcct ttcaacaatg aaaaatggat ttatctctcc attttgaaac
     1681 aagccctttt tctattaatt actttcttaa aaaatataat acgatatata agataaatat
     1741 ctaattgtac ctatttctaa ttatatctat tagaatacaa cacctttgcc ttttcagtaa
     1801 actccctaac aaaatctagt ttactatctg tataagcatc acgattatgc tcatacttct
     1861 tccaaaggtc caacttaagt ttttcatact cctttgcaac atctgggtgg tcaattaagt
     1921 aatctctaaa aaacaactca tcattatcac ctacatacct tatatgcaaa tgaaaaactc
     1981 gttcttcaaa cccctctact gtataccctt tattaagtga aagcctacta ctgctctcag
     2041 acatacaaat atatccattc gactcaatca aatctttaat atctttcata ttataatcag
     2101 gaattatctc aatcagtatg tcaacaatgg gcttggcata aatcgtactc acagaagtac
     2161 ttccaatatg acttattcta acaatttttt gcattggcaa tattccagtt agtctatatt
     2221 gttcttcttt aaaccaattt ttccaacatt ctttgtgagt agtcaaaaat ataggaaaca
     2281 agttccataa ctcctctaaa gtcatatctt ccaacttctt tttcattagt tacctccttt
     2341 taaatttgta ctttatattt tttctttttc aaatttactt agttaatatt taactttatt
     2401 ttttatcaaa tcatttttct aattatgtaa atttttctaa atttagtttt taatgtatta
     2461 taacagattt ttacccttct tacacttttg tatctattta aatattagaa cattttttct
     2521 tacttctaaa tacagtaaga ttgcttaaat accaaatagg agcaaaaaaa ctctgtaatc
     2581 taaaaaaaac tacaacatct ttatgagaat taaaaaatga tagactatgt tatcaacatt
     2641 aagaaacaga tagaaaagaa acagagtatg tgctaaaatg ggaacataga acagaatgac
     2701 aaattggaat ttatggagga cattaaatat ggagtatata atcaatcctt gtagtggtat
     2761 aaaaattaaa ataactagag ggcagaaaat tgaaattgtg gatattgaag gaggacaggt
     2821 cgttgatttt tttgcagaaa tgaaagatga ttcaactgaa tttatatctc caggtgttac
     2881 aattgattgt aatgagtcat taagattaca agttgggaat ttaatatatt ctaataagta
     2941 tagacctatg tttaaaattt tatatgatga tgtaagggaa catgacttgc tacatccatg
     3001 ttgtagacct gagatgtacg atttctttta tcacaatgga gaaaatcatc caaactgttt
     3061 tgataatatt aatcgcacac ttggaacttc ccatcaaatt attcacccca ttaatttgtt
     3121 tatgtacaca aaagtcaata cagatgggag tattgaagta tgtgaacctt tatcaaaagc
     3181 caatgacaaa attattcttg aagcattgga aaacatgact ttagggattg ctgcatgcag
     3241 tgtttcagaa agttcttgta atagcagaaa atgtacttct attaagattt tggttgattg
     3301 acaaatttca tttatcgaaa aaatatcaaa attaaaaaat gacgtcaaag gcgcaaaagg
     3361 gtaaccactt gcgctctcaa ttcattatgg tttgttacgt atttatgggt aaaaatccct
     3421 tgattctatg atgtttttca gacacaatta caaatgagta aggattttat actcatactc
     3481 taaaaaatag cattttattg cgtaacagaa cgaaaaaaca gtgtgtacct ttttcttgca
     3541 atacacactg tattcatagc cactctagct gttaactgtt aattatctca taataccctt
     3601 actggtattc atctaatctt aacccctttt aattattttc atttataaat aaattctatt
     3661 cttcccaaaa tatcgtaatt aaaatatatt aattaaaata tcccaataag aatatctgaa
     3721 aaatcttcta cctatttttt caaacctttt attcccattc ctcatacaat agtgcaactt
     3781 ttagcacagt ataaatatac cattagtcca tataatctca ttaacaaata tatacaatta
     3841 ttttcttcaa actcttttat atctaagata tcacattttc attttcttat attaattcct
     3901 taactctctt tcaaattacc aattttttaa tcaaatcttc attaaatatt ctatcattat
     3961 taaacattca tcattatccc taaaaaatat gtgccgtatg ttctttacac aaattttcca
     4021 tcttatcata cacagcacca ctatctctct catctgtcat agtcacaatt acatcacttg
     4081 gtctaagaat tgtcttacct ctaggaataa tttcttctcc acctctttga attgctacca
     4141 aaagacaatt ttcaggccac tgtacatcct gaattgcctt gttcgcaaca ggagagccaa
     4201 acataacaga gaactggtct agtatctttt cgcccctctc tttatgtaca ggttctccat
     4261 tcttttcaag taacctctca agaagacttt cataaatagg tttagacttc ataagtgtag
     4321 ccacaatata tgccacaatt gaaattactg atagtgaaag catttgacta acagaacctg
     4381 tcatttcaaa aattaatatg ataccagtca aaggggcacg cacaatagct gtaaaatatc
     4441 ctgccatagc caaaagcaca aagttattta cataatcggg attcattcca aaaactttaa
     4501 cacctaccat accaaatatt ccacctataa atgctcctaa aacaagcaat ggaaaaaata
     4561 ttccccctgg tgcacctgac ccaaaactaa cagccgaaaa tataaaacgt ataacaaaaa
     4621 tcaaaacaac cattccaaaa atatatttat gacttgtaag ttcttcaact aaattgtgtc
     4681 cactccccag aacatcaggc ataacaagcc caagaatacc agccattata aatggaatca
     4741 acagttttcc aaacgtacca attttaggaa tcttcttgta taaagactgc acatatagag
     4801 taaaccagtt gtaaaatgcc cccataactc caagaataac acctaaaatc actattaatc
     4861 cataataatc ttgtggcaac acatttccaa
//
LOCUS       DAYASV010000198         4859 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYASV010000198 DAYASV010000000
VERSION     DAYASV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.203"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            96..2798
                     /locus_tag="ACGI68_003565"
     CDS             96..2798
                     /locus_tag="ACGI68_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0139967.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            2832..3530
                     /locus_tag="ACGI68_003566"
     CDS             2832..3530
                     /locus_tag="ACGI68_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0139968.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            3887..4843
                     /locus_tag="ACGI68_003567"
     CDS             3887..4843
                     /locus_tag="ACGI68_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFH0139969.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
BASE COUNT         1973 a          485 c          825 g         1576 t
ORIGIN      
        1 ttacataatc ttaatcatta aaaatacatc ttaactacaa ttttatatcg gaattgatat
       61 tataatagta atgaatggtg taaggaggtt ttttaatgga tagacctaat cctgatattc
      121 tattagaaaa aattaaaaat gaagaagaaa aactaagtag aggtcaatta aaaatattct
      181 ttggttatgc agctggagta ggaaaaacct actctatgtt ggaatctgca caaaatttaa
      241 aaaaagtggg tgtagatgta gtagttggat atattgaacc acacactaga ccagaaacat
      301 tggctttact tgatggcttg gaaattcttc ctgtaaagga gatagaatat aaatctataa
      361 aactaaaaga atttgattta gatgctgctt tagcaagaaa accagaagtt atattagtag
      421 atgaatttgc ccattctaat gttgctgggt taaggcatac aaaaagatgg caagatatag
      481 aagaattgct gttagcaggc ataaatgtct acactactgt aaatgtacaa catttagaaa
      541 gtctaaatga cattgttgag ataattacaa acgtatcagt taaagagact ataccagata
      601 agtttttgga tacaaataca cagttagaac ttatagatgt tgaacctgat gtacttcttg
      661 aaaggttcaa tgaaggtaag atatataaga aagaacaagc tactagagca aaaaataatt
      721 tttttataaa agataacctg gttgcactta gagaaattgc acttagaaaa acagcagaga
      781 gagttaataa agaagttcaa atgaccagac tatcaaaggg tgatgtaact gtaataccta
      841 catcagatac acttcttgca tgtataagtc catctccatc ttcagcaaaa gttattagaa
      901 ctgcatccag aatatcagac agtacatttg caaaatggat agctttatat gttgaaactc
      961 caaacacagc aagacttcca tttgatgaac aaaaacaact acaaaataac ctaaaattag
     1021 ctaagaaatt aggtggagaa ataattgttt tacatggtga aaatataata gagcaaatac
     1081 ttagaattgc aaagctgaga aatgtaacaa aaattgtaat aggtagaaat cattcaagta
     1141 ataaaagatt tagtaaaaga tttaaaaaag atatagttga caaattaatt gatgaagttg
     1201 attatataga tatacatata attccatata aatttgcctc agatgttaaa tacaaaccta
     1261 aaaaggataa atcatcaata aaatctaaat ttaaaattag caaagttgat tttataaaac
     1321 ttatatttat tactttgatg ataagtattt tagcatatgc agtacaaagc atgggattta
     1381 taagagaaaa tatattgtta atatatatgt tgggtgtagt tttagtttct atgtggacta
     1441 aagggtatag tacaggtata ataagttctg tatttaatat agttctttta aattattttt
     1501 ttacagcacc attatataca ttatctatag cagattcaaa ctatatagta acactggttg
     1561 tattctcaat agtaggtata ataactagca ctcttacgtc taagatacag catgaagcag
     1621 aaactgctgc taaaagagaa gaaaatacaa aaatgatata tcaaataatt aagggatttt
     1681 taagattatc aaataaagat gatatagtaa acaaaggtat agaacttttg tctcttagct
     1741 taagtagaga tgtaatttgt tatttaaatg tagataagca taataaatca aagctttaca
     1801 aaaaaaatac taataataag gataaaaatg atttggatag tgaagatgaa aaagctgttg
     1861 caagttgggt tttatccaat tcaacagtag caggaaatga cacggataca cttcctggtt
     1921 caaagggata ctatatacca attgtaggaa tgaatttaac tttaggagta gtgggaatat
     1981 catgtattga ttcaaaattg gatacagaag atataagctt aattgaaaca ataattgctc
     2041 aaatggcaat agcgcttgat agagaaatct tgtcagaagc taaagaaaat accaatcttg
     2101 agattgaaag ggaaagatta agaagtaatc ttttaagagc agtttcgcat gaccttagaa
     2161 ctccactagc aggaatatct ggtgcagtaa gtacaattat aaaaaacaag ggtactatag
     2221 gtcaagatat aatagatgaa ttacttagtg gtgtatatga agatactcag tggttaataa
     2281 gacttgttga aaatttactt agtatgacca agatagatga agggaaattg gaagttaaaa
     2341 aacattctga actggttgag gaaattatat cagaagcgtt acaaaaaata aaaaaaagaa
     2401 ttgaaaatgc acttatagat attaatatac cagaacagat attatttgtt cctatggatg
     2461 caaaattaat agaacaagtt ttaattaatt taatagataa ttctttgaaa tattcaaagg
     2521 aagattgtaa gatagagata aatgtatatg agaaagatga ttatgtttgg tttgaagtat
     2581 ctgataatgg tcctggtata tcaaaggaac ttaaaaagca tatctttgac agatttttta
     2641 ctggagaaga aggagctaaa gattccagaa agggtgttgg acttggatta tcaatttgta
     2701 aatctataat tcaagctcat aaaggtgaaa ttatggtaga aaacaataaa gataagggtt
     2761 caacttttaa gtttagtttg cctaaggaaa atgaatagtt ttaaatttta aaattagttt
     2821 tggaggaaga aatgagtaaa aaacttatat tgttagtaga ggatgataag acaataagaa
     2881 aatttatatc tactgcactt ttaacacagg attatgatgt caaggaggct ataactggaa
     2941 aagaaggtat atctatagct gtctcatatt ctccagatgt tgttctttta gatttaggct
     3001 tagaagatat ggatggaata gaggtaataa aagctatcag acaattttca aatataccaa
     3061 taattgtagt ttcagctaga gagcaagata gagacaaggt agaagtattt gatgcagggg
     3121 cagatgatta cttaacaaaa ccattctcca tagtagagtt attagcaaga gtaagagtag
     3181 catttagaca ttcacaagta gaagctcaac aaaaagatga tgttaaatct acgtttgaag
     3241 tagataaatt attgattgat tttgataaaa gaaaagttat tgttgatgat gttgaagtcc
     3301 atttgacacc tattgaatat aatattcttt cattacttgc aaagtatcat ggaaaagtgc
     3361 ttactcataa ttttataata aaagaaatat ggggtagtgt tatagggaat gagactaagt
     3421 ctttaagggt ctttatggca acgttaagac gaaaaataga aaaacaacct gcaaacccaa
     3481 gatatataat tacagaagtt ggtgtaggat acagattaaa tgatgaatag ttgtaatata
     3541 ttaattatta gattatgtgc ttattaatat atattaaaat tatatagttt agttaaaata
     3601 agaatgaagt gtatgtgctt gttgtcagtt tagcacatgc attttatttt ttagcttttt
     3661 tagtatcaat aattttttag tatcaataat atgaaatatg ttttaaaata agtatatagt
     3721 gtaatatgta ataaaaactt tggttaaata ataaatgtat ttaacactta ccttataata
     3781 aatttaatat aaatattaaa taataattat taaaatagtg taaaataatt acagtaagga
     3841 ttataaaaaa tttttagaat gtaaattgaa atttaggagt agatgtatgt atatactgaa
     3901 tttaaataca agagagacta ttgaagattt tagagataaa ttttatgtag cagaaaattc
     3961 ttatcttata ttaagtgctc caaaaaattt aaaattgctt aaggaaactt tggatataga
     4021 tgaaataact tttaatgatt gtcttaaatt tgatgaaata accaaacttg atttatttga
     4081 taattacgat tttttaagcc taaatacatt tgaactaaga gatggagaag ctgttataga
     4141 agaggtaaat atgtatttat cagataattt tatattggtt gtagtgaatg aagaacactt
     4201 tttatttgaa tttgtaaaaa atattatttt gaagaactct caacttgaaa aaaatccagt
     4261 tattaatcta tttaagataa actacttgat tcttagagaa gttataaaaa atggttttga
     4321 gtctttggaa aaagttgaag agttaattct tcaaatagaa gatgaaatga tggacaacat
     4381 taataagaat catgtatcta gaataagtga tgtaagagga ttaacaagaa ttatagttaa
     4441 gaatactaga cctcttttgt atattgggga tagaatagtt aaggaaaata taagatattt
     4501 aaaatattca aatgtaaaaa aatataattt agaaaatttt caaggtatag attttggaat
     4561 tgacaaatta tacaatttcg cattgtcaac acgagagtta gcagataaat tattagatat
     4621 ttattcttcc agagtaggtg agaaaacaaa taacttaatt acaaaattaa ccttgttaac
     4681 agccatatca gcaccactta caattataac tggtatatat ggaatgaact ttagatatat
     4741 gccagaatta aactggattt atggatatcc agcaacttta ttttttatgc tatgtataat
     4801 ttttgttgga attataatat ttaaaataaa aaaattattg taaattacag atgatgttt
//
LOCUS       DAYASV010000199         4816 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYASV010000199 DAYASV010000000
VERSION     DAYASV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.204"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     regulatory      39..140
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            323..895
                     /locus_tag="ACGI68_003568"
     CDS             323..895
                     /locus_tag="ACGI68_003568"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFH0139970.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            959..1609
                     /gene="fsa"
                     /locus_tag="ACGI68_003569"
     CDS             959..1609
                     /gene="fsa"
                     /locus_tag="ACGI68_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFH0139971.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            1761..4685
                     /locus_tag="ACGI68_003570"
     CDS             1761..4685
                     /locus_tag="ACGI68_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0139972.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKDPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIESEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVMKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         1951 a          439 c          931 g         1495 t
ORIGIN      
        1 ttgttgaata atagagaatt ataatatata attaaaagga ataaattaat atatttacct
       61 gtatatgttt gataatatgg ttcaaatgtt tctactaagc taccattaat agcttgacta
      121 caggagtgaa gaagatatag tttatactta ttttaaatta tatattaagt taattacttt
      181 gaattttaag ctcctgtcta tgatatagac aggagctttt ttagtatttt aatataaaat
      241 acttacaaga atagatagca aaattagaac aaatataatt tttttattaa tataatttta
      301 gaaagggcta ggtggaagtt caatgaaggc tttacaagaa aagatattga gagaaggcag
      361 tgtttcagga aatgatatac taaaagttga tagtttttta aaccatcaaa ttgatgtagc
      421 ttttttaaat gaaataggaa gagaatttaa agagagattt aaaggagaaa aggtggataa
      481 gatatttaca atagaggctt caggaatagc tatagcatca attgtttcac agtattttga
      541 taatgcacct gtagtatttg caaaaaaatc agaatctaaa aatttggata aagatgtata
      601 tgaaactaat gtatattcat ttactaaggc aagagagtat tcagttaaag tttctaaaaa
      661 gtacataaac aaaggcgaaa atatattgat agtggatgat tttttggcaa atggaagagc
      721 tgctcttgga cttaaagatt taattgagca agcagaagct aacttagttg gagtaggaat
      781 tgtcattgaa aaaggatttc aagctggagg agcactttta aaggctaatg atgtaagatt
      841 agagtctttg gcagtagttg aatctataga taatggaact gtaaaattta ggtaatttat
      901 gatattatat acttgtgttt agcacatatt ttaactatgt aattatggag gattttttat
      961 gagatttttt atagacacag ccaatattga agaaataaaa gaagcaaatg atttaggtgt
     1021 aatctgtggt gtaactacta atccgtctct aatagctaaa gaaggtagag attttataga
     1081 ggtagttaaa gaaataagtg aaatagtaga tggaccaata agtgcagaag taataagttt
     1141 agaacataaa ggtatgatag aggaagcaga aaaattatct aagatacata aaaatatagt
     1201 tataaagata cctatgacag cagaaggtct aaaagcggtg aaagtactat caagtaaagg
     1261 tataaagact aatgtaacgc ttatattctc tgctggacaa gctcttttag ctgctagagc
     1321 tggagctaca tatgtaagtc cttttgttgg tagattggat gatatatcac aaaatggtct
     1381 tgatttaatt gaagaaatcg ttgatatatt tagtgtaaat agtattgagg ctcaaataat
     1441 agttgctagt gttagaaatc caatacatgt tttacaagct gctagaatgg gagcagatat
     1501 agcaacagtt cctcttaaag ttataaacca aatgattaag catcctttga cagacaaagg
     1561 aatagatagc tttatgaagg attgggaagg tgcttcttta aaattataat tataaactac
     1621 gctttataag tatattaaag aggttattat ggaaattcta agtaataagg ctaggctatt
     1681 ttgcctagtc ttattttggt gtataatata ttgaaacata ttattgaaat agattaattt
     1741 tttaagtgag gaggcgaaaa atggataaaa tattaagaga gatacaaaga gaagataaaa
     1801 aaaatccata tactgaccaa gaacttgctg aaatgttaaa tgtagctagg tctgaagtga
     1861 tatcagttag gaaaaaaaat aatattttag attcgagaga aagaagaaaa aaaatattaa
     1921 taaaagatat atcaaaaatt gtaagtgaaa atcctcaaat gtctgaaaga aaaataactg
     1981 aaatattaat agaaaatgga tataatatat cgagaagtgc agtttcaaaa ttattaaaag
     2041 aggaaaattt aaatcagtca aagattggga ttaaggataa agacccagca tcatcaggaa
     2101 agtatgtgaa ggtgagtaaa gttgtagaag aagatggatt tgaagaattg ataggtataa
     2161 agggaagttt gaaagaaaag gtaaatctag ctaagtcagc aataatgtat cctcctaatg
     2221 gccttcatac tataatatat ggagaaacag gagtaggtaa aagtgaatta gctacatgta
     2281 tgtataaata tgctataaaa aacaacatta aagaagagaa cagcccattt atagtgttta
     2341 actgtgcaga ctatgcagaa aatcctaatc tattgatagc tcaactcttt ggggtagtaa
     2401 aaggtgcata tacaggagca gatgcaaata gagaaggtct tgtagaacaa gctaataatg
     2461 gaatattgtt tttagatgaa attcatagat tgcctgctgc tgggcaagaa atcttatttt
     2521 cattaataga tagaggagaa tttagaaggt taggagaaag tagttctaat agaaaggcaa
     2581 atgttctcat aataagtgct acaacagaaa atccagattc taatttactt caaacattta
     2641 gaagacgtat accaatggta ataaatttac ctaatatgag tgaaagacca aaatctgaaa
     2701 gatatgaaat aataataaag ttttttgaaa gagaagcaaa gcgagtaaac aagaatttta
     2761 taataactaa agaagttatg tgtgcactta tgaattataa gtgtactgga aatataggtc
     2821 agttaaagag tgatatacaa gtaacttgtg ctagagcttt tagtaaatcg atattttcta
     2881 gcgataaagt aattatagat ttagatagtt taagggatta tataaaaagt ggatattatg
     2941 attgtaattg taaaaatgaa aagtattctg agttttcagt tgaggatata tatatagata
     3001 ttagtgaatt ggtgagtgga aaaaatagaa ttttatctga taatgagata agcgagatat
     3061 ataattatgc agaaaaagag cttaaggttt tggaaagtaa gtgcttttca gaagaagagt
     3121 taaaaaatgc atttatagaa aagttagata agaagtttaa tgctattaaa aacaataaaa
     3181 atttatctga tagaagaagt cgaatagact ggggaggaca attaaaagag tctacactgg
     3241 agataatgga taaggtaatt gtatttatta aggagcagtt taagcatgta aatcaaggtc
     3301 tgtacttggc tctagcaatt catattgagc atgctatagg taggataaaa gatggaaaaa
     3361 ctataataaa tccgagttta gataaaataa aagcatcaat gccaatggaa tatgagttgt
     3421 caagatatat aatgggtata gtagaagatt tgacacaagt aacatttcca gaagatgagc
     3481 ttggatattt ggcttattat ataaataaat tttgttataa tgaggaaagt ataaaagata
     3541 aagttaaagt agtaatagtt acgcatggta aagttggaat tgaaatgtct aaagttgtaa
     3601 atcatatttt aggtatagaa tgtacactag gtatagaaat agcacttaca gattcaccat
     3661 cagaggggat agagcatgta ttagaagaac ttcaaaagat agaagctaga aaaggtattc
     3721 tagttctaat tgatatgggt tctttggtaa ttcttggtga tgaggttgaa aaaaggcttg
     3781 gaattagatg taaaactgta aatagagtag atacattatt ggcaatggaa gcaggaaaac
     3841 ttgcaacaat tgaaggaaaa agtttagatg gtataatagc tgatttaaag aaaaataaga
     3901 attatgctat gataaataca aataaattta gttacagaaa aaacgagtat ggaaagaaaa
     3961 atgtaatcat tactttatgt ttgagtggtg taggtacagc tttaaattta aaagagcata
     4021 tagaaaaaca aattaaggaa tatgacactt taattgaagt aaagccagtt gcttttttaa
     4081 ataataattt agaagaagag ttaaatgata ttgaatctga atataatatt attgctatct
     4141 gtggaactat tgatatagat tataaaaacg tgccttttat ttcttataat gaggtacttg
     4201 gtaaaaatgg tattaataaa gtattagagc atttaaatag taaacaacca gctttaaata
     4261 ttgaaaacca gttgaataat ttaatacatg aagatttgat tttatacaat tttgaaggaa
     4321 tttcaaagga gtatataatt gatactcttg tttcaaaact tgaagaaggt ggatatgttg
     4381 actcaaagta tatattaagt gtatataaaa gagaagcaat gggaagtgtt gtaatggcct
     4441 ctaaggtagc cgtgccacat ggacttccag aaaacgttat gaagcctgca atagcaatcg
     4501 cgaggttgaa taaacctata gtatgggaca ataaatttat ggtggatttg gtggtactac
     4561 tggcacttaa agaaaataat aagaaagaaa tacgaagctt attttcaaag attaatgacc
     4621 agcatacttt agaagtttta ttaaatactg aagataaaaa tgaaataaaa gaacttttaa
     4681 gctaattatt agtaccgaga agaaagacaa aaaaatttgc tttagtataa aatttaaaac
     4741 tactcaattg ctaagtagtt aattactcta tataaggcta gttctatgaa atttatttat
     4801 atgattttat agaatt
//
LOCUS       DAYASV010000200         4655 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYASV010000200 DAYASV010000000
VERSION     DAYASV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.205"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            726..1388
                     /locus_tag="ACGI68_003571"
     CDS             726..1388
                     /locus_tag="ACGI68_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0139973.1"
                     /translation="MSILEIKNLNKIYGKSETEVKALSQINLKINSGEFVVIVGKSGS
                     GKSTLLHIMAGLETPTNGDVIIDNINISSLDEKKRSALRKEKLGLIFQSYNLIPVLTV
                     EENIKLPTINIKNKDEAYINELMELLGIKDRRMYLPNQLSGGQQQRVAIARALVNKPS
                     IVFADEPTGNLDTKTESDVLSLLKESQKKYNQTIIMITHNIDITKYADRVIEIEDGFI
                     RE"
     gene            1397..3904
                     /locus_tag="ACGI68_003572"
     CDS             1397..3904
                     /locus_tag="ACGI68_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0139974.1"
                     /translation="MKLNYIVKKYIMGNKKNTLLIIVSIVISTALFLIMNIISEDATN
                     LMINQAKNEFTLKHAQYINPKDEEIKYIENNTSIDKVGKSMLLGISDIGKGQTLQILS
                     QDKVAEELNDIYTLEKGKLPVAENEIAIDSWYIKQKKIKNPIGKRITLDYRRQGIDQE
                     DLYTGEKEFKITGILNSNPILKAQGTSIGLISNDCAIKNIPTKNKYDQVMFTFKKEKN
                     IQRQSQKLIKNGNLNENNIYFNNELLIAISDSMNLKIPYIIVNIVLALATILLIYNIF
                     YILVSNRIKDFGIFRALGFNPNDIFKIMILEVSIYSIISISIGLILGGIIASLSREYV
                     IGVIYNVNYINSIKNQNYINTYIISILLSLGTIIISVSKPLMLSVKTDPMICIRRNNE
                     KVDIKQNSFINKFMIKLFKDYGNIASKNIQRNKKRTNLSIASMVIIFFLMATIYTKST
                     SNFLSDGVLKYWIQGDYLLHNIDISTIRSNNKGYDKNVLQEIKAIDGVTKVNSYRHKW
                     FNIKIDDKSINKNSDYWKKNKDNIEARSEVKDGIKVYNNSFEFLGIEDTDILEDFLIN
                     GKENIDKFSKQPYLYITKKSSDSLNIKKGDRIKVYFDIIDSKTNNFTKTISQEFIVCG
                     IITTLPVTSQVGAEFGGVISTNQFNKFTGISSYERFDIWTSKLANDKYVESELNKITE
                     KTNKGILIPYKAETAEYEKIDNQKTLIMVLVTGIIVILSLFNCCNTIVTSINSRSREF
                     ALFRGIGISKHEINKIVKLESYIYIIVSFCISIIPILIVRSIIIKPFESINLINWKFI
                     GAIILIVFILVSIIMITTLKTLNKIQNENFMEEIKTL"
     gene            4235..4579
                     /locus_tag="ACGI68_003573"
     CDS             4235..4579
                     /locus_tag="ACGI68_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139975.1"
                     /translation="MYCLSVVLAIYGIYVFINCTEYIYTMLSTKHLTVKGNEYTIINY
                     YMSNFSQYIIFAILLASMGFVIQKIVHMHFSEIELSGNYSTTVRNISDISLDNDNEMF
                     NRGNIENSILKK"
BASE COUNT         2052 a          409 c          693 g         1501 t
ORIGIN      
        1 tatcatatga aacctttata gattatgaaa gcaataatat agtgtatgat atataaaata
       61 agttataaaa gtattttttt agatattcaa aaagacattg taagttatat taataatatg
      121 ggtatcaacg aattttggat ataatgagtt tttcaactta aagttatgta ataatgaatg
      181 taaagaatat ttaatgaaat cattttgttg gttatgtcta gctgaataca ataatctttt
      241 agataaagat tttatatttg aagataggtg tgaaaaaatt aagaagatat taacatcagc
      301 agtttagagg caaagcatgt aaagtaaagt ctgggtaact aaaagtagta atttagattg
      361 taactattgt tataaatatg aaaatgagga aacaggaaat ataagttagg atgtagaaaa
      421 taggttacta aataaaaagc ttaattatat aatcaaagaa ataggtatat actgtacaat
      481 ttcatggtga agaacttttc ctaaataaaa aggttggtaa aagagatata agaaataaaa
      541 ttcaattata agatatggta tgataactaa aggcactata tggagtgaat aggcggaaga
      601 attttttgaa aattataaaa atagtttctg ttgctacata tcaataagta tagatagaga
      661 ttgcaaaatc catagcaaaa atcgaattta taagtgtaaa taacatgtaa gacaaggagt
      721 aaattatgag tattttagag ataaaaaacc taaataaaat atatggtaaa tcggaaacag
      781 aggtgaaagc gttaagtcaa ataaatctaa aaataaatag tggagaattt gttgtgatag
      841 taggtaaaag tggaagtggg aaaagtactt tactacacat aatggcagga cttgaaactc
      901 caacaaatgg tgatgtgatt attgataata taaatatatc atctcttgat gaaaagaaaa
      961 gatctgcatt acgtaaagaa aagttgggat taatatttca atcatataat ttgattccag
     1021 ttttaactgt tgaagaaaat ataaaactac caactataaa tataaaaaat aaagatgaag
     1081 cttatattaa tgaattgatg gagttactag gaataaaaga tagaagaatg tatttgccaa
     1141 atcaattatc aggtgggcaa caacaaagag tagcaatagc aagggctctt gtaaataaac
     1201 catcaattgt atttgctgat gaacctactg gaaatttaga cacaaagaca gagtcagatg
     1261 tattaagttt attaaaagaa tctcaaaaaa aatataatca gacaataata atgataacac
     1321 ataatataga tataacaaaa tatgctgata gagttataga aatagaagat ggatttataa
     1381 gagagtaggt agatttatga aacttaatta tattgtaaaa aaatatataa tgggaaataa
     1441 gaaaaataca ttattaataa ttgtaagtat tgtaatttca acagcgttgt ttttaattat
     1501 gaatataata agtgaagatg cgacaaactt aatgataaat caagctaaaa atgaatttac
     1561 attaaaacat gcacaatata tcaatccaaa agacgaagaa ataaaatata tagaaaataa
     1621 cacaagtata gataaagttg gaaaatcaat gcttttagga attagtgata ttggtaaagg
     1681 tcaaacacta caaattctat ctcaggataa agttgctgaa gaactaaatg atatttacac
     1741 actagaaaaa ggtaaattac cagtagctga aaatgaaatc gctatagatt catggtatat
     1801 taaacaaaaa aagataaaaa accctatagg aaaaaggata acattagatt atagaagaca
     1861 agggatagat caagaagatt tatatactgg ggaaaaagaa tttaaaataa ctggtatctt
     1921 aaatagtaat cctattttaa aagctcaagg aacatcaatt ggccttataa gtaacgattg
     1981 tgctatcaag aatattccta ctaaaaataa atatgatcaa gttatgttta cttttaaaaa
     2041 ggaaaaaaat atacagagac aatcacaaaa acttattaaa aatggaaatt tgaatgagaa
     2101 taacatttat tttaataatg aattattaat agctatatct gatagtatga atttaaaaat
     2161 accttatata atagttaata tagtactagc actagcaact atattattaa tttataatat
     2221 tttttatata ttagtatcaa atagaataaa ggattttggg atatttaggg ctttagggtt
     2281 taatccaaat gatattttta aaataatgat tttagaagta tctatatact ctattataag
     2341 tatttctata ggtctaattt taggtgggat aatagctagt ttatcaaggg agtatgttat
     2401 aggagtaata tacaatgtca actatataaa ttctataaaa aaccagaact atataaacac
     2461 atacataatt tctatattat taagtttagg aactataata atatcagttt caaaacctct
     2521 tatgttatca gttaaaactg atccaatgat ttgtataaga agaaataatg aaaaagtgga
     2581 tataaaacaa aattccttta taaataaatt tatgataaaa cttttcaaag actatggtaa
     2641 catagcatca aaaaatatac aaagaaataa gaaacgtaca aatttatcta tagcatcaat
     2701 ggtaattata tttttcttaa tggcaacaat atacacaaag tctactagta actttttaag
     2761 tgatggtgta ttaaaatatt ggatacaagg agattattta ttacataata ttgatataag
     2821 tactatacgt tcaaataata aaggttatga taaaaatgta ctacaagaga taaaggcaat
     2881 tgatggtgta acaaaagtaa actcatatag gcataagtgg tttaatataa aaattgatga
     2941 taaaagtata aataagaact ctgattattg gaaaaaaaat aaagataata ttgaagctag
     3001 atcagaagta aaagatggaa ttaaggtata taataattct tttgaatttt taggaataga
     3061 agatacagat atactagagg atttcttgat aaatggaaag gaaaatatag ataaatttag
     3121 taaacaacca tatctgtata taacaaagaa atccagtgat tcgcttaata taaaaaaagg
     3181 agatagaata aaagtatact ttgatattat agattcaaaa acaaataatt ttaccaaaac
     3241 aatatctcaa gaatttatag tatgtggaat tataactaca ttaccagtaa cctcacaagt
     3301 gggagctgaa tttggcggag taatatcaac aaatcaattt aataagttta caggaatatc
     3361 atcttatgaa agatttgaca tatggacgag taaactagcc aatgataaat atgttgaaag
     3421 tgagttaaac aaaataacag aaaaaacaaa taaaggaatt ttaattccat acaaagctga
     3481 aacagctgaa tatgaaaaaa ttgataatca aaaaacacta attatggtat tagtaactgg
     3541 aattatagtt atattatctt tatttaattg ttgcaatact atagttacta gtattaatag
     3601 cagaagtagg gaatttgcac tatttagagg aataggaata tcaaaacatg aaataaataa
     3661 aattgttaaa ttggaaagtt atatatatat tatagtaagc ttttgtatat ctataatacc
     3721 tattttaatt gtaagaagta taataataaa accttttgaa agtatcaatt taataaattg
     3781 gaaatttata ggggctatta tactaatagt atttatttta gtatctataa tcatgataac
     3841 tacactaaaa acattgaata aaattcaaaa tgaaaatttt atggaagaaa taaagacttt
     3901 atagtgaaca gcctataaat taaagaggtt ttatatgaat ttaattgcaa ttatcgataa
     3961 tggtgtagat attgatttta aataatttag aaataaaaat aaaataagtt atccaagtta
     4021 tatgtatgat gtaatagggg tacaatgtat agcaaatata aatattggct taataaaact
     4081 ctactcttaa gtctactgtc gttttaacac aagactaata ataagtgtaa cctaaaaatt
     4141 attgataata caataacctt gtttatgata aataacaaat attaagtgtt gtaaagatag
     4201 taagtttaaa aaaatatcaa agatttcaat cacaatgtat tgtttatctg tagtgttagc
     4261 tatttatggt atatatgtat ttataaattg tactgaatat atatatacta tgctatctac
     4321 aaaacattta acagtcaaag gaaatgaata taccattata aattattata tgagtaactt
     4381 tagtcagtat ataatatttg ctattttact tgcatcaatg ggatttgtaa tacaaaagat
     4441 agtgcatatg catttcagtg agatagaact tagtggtaac tattcaacaa ctgttagaaa
     4501 tataagtgat atctctttag ataatgataa tgaaatgttt aatagaggca atattgaaaa
     4561 tagtatttta aaaaaataaa taatataaat atataaaatg tgattgaata aagcttagaa
     4621 tacttagtgt attttaagct ttttttatta ttaag
//
LOCUS       DAYASV010000201         4537 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYASV010000201 DAYASV010000000
VERSION     DAYASV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.206"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            97..1095
                     /locus_tag="ACGI68_003574"
     CDS             97..1095
                     /locus_tag="ACGI68_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFH0139976.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            1457..1984
                     /locus_tag="ACGI68_003575"
     CDS             1457..1984
                     /locus_tag="ACGI68_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HFH0139977.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            2011..2607
                     /locus_tag="ACGI68_003576"
     CDS             2011..2607
                     /locus_tag="ACGI68_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HFH0139978.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEIAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(2672..4255)
                     /gene="asnB"
                     /locus_tag="ACGI68_003577"
     CDS             complement(2672..4255)
                     /gene="asnB"
                     /locus_tag="ACGI68_003577"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFH0139979.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDRYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         1591 a          703 c          652 g         1591 t
ORIGIN      
        1 aaaaattttg tataaaaaat ataaccttat atgctacata ttatttctaa atgaaaaatt
       61 accataataa tttaataatt acaggagggt attgatatga aaatactagt atttggagca
      121 cgcgattatg aagaaccagt aataaaaaaa tggtctgaag aacataagga tgttcaagtg
      181 gatatttatc ctgaaaacat gactgaagaa aatgtagtta aagctaaagg gtatgatggt
      241 atatctatac aacaaactaa ctatatagat aatccttata tttatgaaac tttaaaagat
      301 gctggggtta aagttatagc ttcaagaact gcaggggttg acatgataca ttttgattta
      361 gttaatgaaa atggacttat cgttacaaac gttccttctt attcacctaa tgcaatagct
      421 gaattagctg ttactcaagc tatgaacctt ttaagaaaga ctcctctagt aaagaaaaaa
      481 gtctgtgaag gtgattaccg ttggatagct gaacttcttg gaacagaagt tagatctatt
      541 acagttggtg ttataggtac aggaaaaata ggtgctactt ctgcaaaatt attcaaaggc
      601 ctaggagcta atgtaattgc atttgaccaa tatccaaata gtgatttaaa tgatatatta
      661 acttacaaag attctttaga agaccttcta aaagaagctg accttataac attacatact
      721 cctttacttg aaggaacaaa acatatgata aataaagata ctctagctat aatgaaggat
      781 ggagcttaca tagtaaatac tggccgtggt ggtttaatta atacagggga tttaatagaa
      841 gcactagagt caggaaaaat tagagctgct gcccttgata catttgaaac tgaaggattg
      901 ttcttaaaca aaaaaatgaa tcctggagaa ttaactgacc cagaaataaa taaacttctt
      961 tctatggaac aagttatatt cactcatcac cttggtttct tcactagtac agcgattgaa
     1021 aatatagttt attctagttt aagtagtgct gtagaagtta taaaaacagg aactgctact
     1081 aatagagtaa attagtattt tataaatata ttttttcatt aattagctat tatctaacaa
     1141 aatagccttt aatttcccaa atataagaga gtcagctcag tatatattga gtagactctc
     1201 ttatattatt ttttatatta aacttgtact cttcttattc ataccaaatt aaatttatat
     1261 taacttattc taactactcc tactctatac attttaccta tttttacttt tctaattata
     1321 tacatttgaa tgggtacaat ctgtatttta caaaaaaaac acctaacaaa ctatgaaaat
     1381 tgtatgatta atattaatat atttataaga aaacataaca attatgttag ctctggattt
     1441 ttagggaggg agtatattgg aaaaaatgaa taaagttact tctaaaaaaa ctacctatat
     1501 tgttacttca gcattatttg catccataat atgcctgaca atagcatata tattacatat
     1561 tccagttggt ggaaacaatg gatatgttca tataggagat gcttttattt atctagctgc
     1621 aactattttg ccaacaaact atgctatagc tgcttctgca attggtgctg gtctagctga
     1681 tttatctaca ggtgctgcta tctgggtaat accaactata atcataaaac caatacttgt
     1741 attatttttc acttctaaaa gtgacaagat aataaataag agaaatatag ttgcttcggt
     1801 tgtagcaggt atagtaggat tagttttata catgtttgct gaaggtataa ttattggaag
     1861 ttttacaagt gcatttgtaa tgagcttact tggcttatta cagccaatag gaagttttat
     1921 tgtttttata atcttaggta tggcacttga taaacttgat tttaaaaaac gttattttaa
     1981 ttaagaattt aaatttatgg agggaaaatt atgaatatct tatatacaat tgaaaatagt
     2041 atctatgtaa atataactaa tacatgtcca tgtagttgtg ttttttgtat aagaaacgaa
     2101 aaagatgaag ttgcaaatag tggtagttta tggttggagc atgagccaag tgtagatgaa
     2161 gtaaaagaag ccttcaataa atacaattta gacgactatg atgaaatcgt attttgtgga
     2221 tatggcgaac cattaatgag aataaatgaa ttgatagaga tagcaaagtt tataaaagaa
     2281 aaaagtagca taaagataag aataaataca aatggtctaa gtgatttaat acataacaaa
     2341 aaaacagcta ttctgttaaa aaatgttata gatgcagtat caataagtct taatgcccca
     2401 aataaagaag cttataatag agttactcaa ccaaagtttg gagaaaaatc atttgattat
     2461 atgttagatt ttgcaagaga ttgcaaaaaa tatataaaag aagtagcttt ttcagtagta
     2521 gatgaaatat ctccagaaga aatagaagaa tctaaacaac tggcaaaaaa acttgatata
     2581 ccattaagag ttagacataa gaattagcaa gtaaaattat aaaacattaa gaatcacctg
     2641 aatggtattt caggtgattc ttcctcttgt attaacattt tacaaaatag atttcatacc
     2701 acactaggaa tgtgtaaatc atccaaatct ttctccaatt atcagagttt ccactaatgt
     2761 agtcattaaa aatctcatta atctcatcta tgttaaagaa tttatctgct atctcactat
     2821 tgaatttatc tcggatatcc ttattatatc ggtcatcagc catccatata cgaattggca
     2881 caataaatcc taatttattt ctaaaagcaa tctcttctgg taatgttttt gatgcagctg
     2941 tacgaaaggc aattttattc tcttttcctt tcactttata ttctgatggt attctggatg
     3001 caatatcaaa tattcttgta tctgtaagag gcattctgac ttccaatcct actgcagtac
     3061 tcattttatc tatattaaaa taaatatctc cttccaacca tatttgaata tctacatttg
     3121 acatgttagt aagtgtatct agttcttctg tttcttcata aattcctttt accacttcaa
     3181 taggaagcac atcaggattg tatttcttaa gaatttttct tttctcaccc tcattcataa
     3241 tgtttgtatt tccaatataa aatgtcttca aattctctcc ataacactct gccttatggt
     3301 atatattata tccaccaaaa aactcatctg ctccttctcc agagtaacag atatttgtgt
     3361 tttcagctgc tgccttgcat ccaatagcaa aaacgatagc agatgcatct ccagttggtt
     3421 gctccatatg gtacattaca taaggtactg acgcaaaata ctcttcaggt gtaattatat
     3481 atctcgaatt tttacaccca agaatattag ctgtctgttg tgctaatttt gattcatcaa
     3541 aacgctcttc ctcataacca catgaacctg ttttttctat atctgacatt gccaatatat
     3601 aagatgaatc aacacctcct gataggaata actctgcagt ttcattctct gattttacct
     3661 ctggcataat tttctgaagt gttgtatgaa tttcatccgt ccattcctca atagacttgc
     3721 tgtggtcagg atgaaattca ggtttccaat aacgaccaat acatactgtt ttattctgat
     3781 agacaaggta atgtcctggc ataagcttct ttactccacg gaagaatgta ttttcacctg
     3841 ctacatatgt gaaagacata taaatctgta acatgtcttc atttaactct ttcacaaatc
     3901 ctggttgctc cataatctta cgaatagatg aaccatagag aagcttccca ttttcagtct
     3961 cataataata gaaaggtttt accccaaact ggtctcttag gcagaagagt ttcttttcca
     4021 cagtatccca taatgcaaaa gcaaacattc catgcatgtg atttgccata tcatatcccc
     4081 atgtcttata tgcttccact aaaattgctc gttcacgctc atctctacat aatgaagaat
     4141 cgattccaag ttcttcacaa agaactttcc agtttcgaat atgcccattg tattctagaa
     4201 tttttatcat atatgtgtct tttcgtgtta aatacttttc ttctaaattt atcatatgtg
     4261 tatcctttcg tattaaaaca tttaacttct aaatttatta aatgtttata tatatttatt
     4321 cacttataat cactgaaaaa tcgacttttt tcaaagtaaa ttctttttga attagtacta
     4381 atctgctctt tattaatggc ataaatatga ttatcaagta ttatcagtgt tataacattg
     4441 aattttttat tcttcataaa tattgccccc tttatattac caactaaata taatatatct
     4501 tatgttactt acatattctt taagaaattc tgaagat
//
LOCUS       DAYASV010000202         4529 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYASV010000202 DAYASV010000000
VERSION     DAYASV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.207"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            231..1310
                     /locus_tag="ACGI68_003578"
     CDS             231..1310
                     /locus_tag="ACGI68_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFH0139980.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIVKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="ACGI68_003579"
     CDS             1634..2002
                     /locus_tag="ACGI68_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HFH0139981.1"
                     /translation="MNVYSFWNDTLAQDAVKIRKYFHEDAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVERVLEFGNLVITVTHVYAINKSLSFHVVSFISLKDDKIISIDEYW
                     GEDDDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="ACGI68_003580"
     CDS             2119..2922
                     /locus_tag="ACGI68_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0139982.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDRQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYMGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3314..4204
                     /locus_tag="ACGI68_003581"
     CDS             3314..4204
                     /locus_tag="ACGI68_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0139983.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFDAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDSCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1719 a          491 c          852 g         1467 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagccttta
      601 tcatcaagga cgaagagcgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggct gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacccg tactgcctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgtaaagg attggtacgg taaaaataaa tccactaact
      901 ggattgtaaa gcatggttgc agaactctac taaaaaaagg caatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagcc atataaatta gaatttgtag
     1621 aggagaaata taaatgaatg tatatagttt ttggaatgac actttggcac aagatgctgt
     1681 taagataaga aaatattttc atgaagatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaagg gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaatca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggatga tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtgtttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccag atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gatagacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaattgttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgctgaaa
     2641 atgttagaag gttacgaaaa aaaagaactg tgtcatctca tgtatatggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaataa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaactaca tactcattag
     2941 gtaaattatg tagtatatgt gtttacagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat atataaattt
     3061 ttaagcgaaa tacatcaaaa gaaggattag aattagtgga gacagaacag ctattataga
     3121 ttataaataa atagttttaa aggagaggta ctacgaatat agaggaaggt aaatcatacg
     3181 ttattcaagt attataaatg attagtttaa aagtactatc ggataggagt ctccatattt
     3241 aaaccttagt aaaggtagtt aaagtagtag tttaaaaact aaaaaattat ataaaggaat
     3301 taggtataag aaaatgattg agagtatacc agcaaaacaa attttacaaa aagtgaaatt
     3361 tgacaataca agatggtttg gaattgacta taatatgaat ctctatcgtg gttgttctca
     3421 tggatgtata tattgtgata gccgtagcac aatatacaac atagagaatt ttgacaaagt
     3481 tagatataaa gaaaatgtga tagaaatttt aagtaaagaa ttgcgttcaa aaagaaaaaa
     3541 aggtgtagta ggcattggag ctatgtctga cacatataat ccttttgaaa agcaactttg
     3601 tattacaaag caagcactag atttgatttc ggaaaatcat tttggagtgt ctattgatac
     3661 taaaagtagt ttggtggtta gagatatacc gattttgcag aaaattaaaa aaaataattc
     3721 tgctattgta aagttgacta taaccactgc aaatgatgaa ttatcaaaga aaatagaacc
     3781 ttatgtaaat ccaagttctg tacgatttga cgcagtgaaa gaacttaatg attctggaat
     3841 tttttgtggc atattattga ctcctatgct tccatttttg actgataata aagatgaaat
     3901 acgagcaatt gtagaaaaag cacacaaggc taatgcaaaa tttatttatt gcatgtatgg
     3961 tgtaacaatg cgtagtggtc agagagagtt tttttatgag catttaagag atatatcacc
     4021 aaaattggta cttaaatatc agaaaacata tggtttaaac tatgtatgta ctgtacaaaa
     4081 taaagattct tgtgagaagt tgttaagaga agaatgtgca cgttatggta ttttgacgga
     4141 tatgaaagat attataaaag cgtacaagag gtcggagtca tatactcaaa taaaattttt
     4201 ttaagatagg ctttggatga gatataatag tgttgattga tgtgttgata ttatagttga
     4261 ttgttagaga ggatattata gatgaatatt agtattgata ttttaggtaa ttgttagagc
     4321 tgatattata gatgaatatt agtattgata ttctaggtaa ttgctagagt tagtattata
     4381 gatgaatatt agtattgata ctctaggtaa ttgctagagt tagtattata gatgaatatt
     4441 agtattgata ttctaggtaa ttgctagagc tgatattata atttattatt agtattgata
     4501 ttctaggtaa ttgctagagc tgatattat
//
LOCUS       DAYASV010000203         4523 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYASV010000203 DAYASV010000000
VERSION     DAYASV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.208"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(46..618)
                     /locus_tag="ACGI68_003582"
     CDS             complement(46..618)
                     /locus_tag="ACGI68_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0139984.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(647..913)
                     /locus_tag="ACGI68_003583"
     CDS             complement(647..913)
                     /locus_tag="ACGI68_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFH0139985.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(913..1122)
                     /locus_tag="ACGI68_003584"
     CDS             complement(913..1122)
                     /locus_tag="ACGI68_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFH0139986.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1391..2251
                     /locus_tag="ACGI68_003585"
     CDS             1391..2251
                     /locus_tag="ACGI68_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFH0139987.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2299..3327
                     /locus_tag="ACGI68_003586"
     CDS             2299..3327
                     /locus_tag="ACGI68_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFH0139988.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVVYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
     gene            3698..4204
                     /locus_tag="ACGI68_003587"
     CDS             3698..4204
                     /locus_tag="ACGI68_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HFH0139989.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT         1775 a          483 c          714 g         1551 t
ORIGIN      
        1 taaattataa attatcaaat aataatagtt atacaaataa aaattttaga acattttttg
       61 agattcttct aactcgtaag catccattaa agcttcacca aatctttgat agtgaattat
      121 ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg ttaggtttat
      181 aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat gtaaatcggt
      241 tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat ttgaatctgt
      301 tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag cttttaactc
      361 ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct caacgtgagc
      421 tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag gcattgtata
      481 tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac caaattgagt
      541 tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt gttgtatagt
      601 tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa taattaaatt
      661 ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt ggagcatacc
      721 caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc gcaaattgat
      781 tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg tctaaatata
      841 agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt tcagctctag
      901 atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat ataattcttt
      961 gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa attttaatgc
     1021 atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc tagccaaaga
     1081 tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa cctccttgtt
     1141 gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa atcgttacta
     1201 ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg ttatttatgt
     1261 tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta aagtaaaaat
     1321 tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca taaatatacg
     1381 aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc cttttgagga
     1441 aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagaa aaaatttatg
     1501 gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg ataagatagc
     1561 tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc aagagttaaa
     1621 aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg gatatcatgc
     1681 aaatataaga caattactta tagataattg tgagtatcta aatataccaa tacatttatt
     1741 tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat gctatatgtg
     1801 tgcaagaatg aggagaggag cattatattc aaaggcagaa gaactagggt gcaacaaact
     1861 tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaact tattgtgtgc
     1921 tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag gtataaaaat
     1981 tataagacca ctttattata taagagaaga acatattata agatttattc aaaatagtgg
     2041 tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta ataaaagata
     2101 tgagataaaa gacttaataa agagtttaga atcaaatttt aaaaatgtag aaaaatctat
     2161 atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa aagatgggga
     2221 gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca ctataaacaa
     2281 ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa tatgccaaat
     2341 tctaaatcaa aaagagcagt atttaattct gatataattg caattggagc agttgtgtat
     2401 gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt aagtaatgag
     2461 gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt gaagagtgca
     2521 ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt ttctgatatt
     2581 aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac agatatgaga
     2641 agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt atttgtagat
     2701 ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc attgaaaaat
     2761 ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt aaatgatgca
     2821 tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca aaatctttta
     2881 ataatacgtg acataataag accaccattt aacgatataa attctaattt agataattgt
     2941 tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat tgtagatttt
     3001 tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga tgtaaatata
     3061 caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga taaattgaaa
     3121 gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgacat gaaagatgta
     3181 ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga gataaaaaat
     3241 ataataaaaa tgtttgatga agacttagat aatgaaaaaa tatatataaa taaatgtaat
     3301 aacctaaatg ttgttaataa gatataagaa attataaata atagagtaga acttgacaat
     3361 tataacaatt ttttataaaa ctgaatcaag ataaaattaa attaatataa aaataatata
     3421 caataaaaaa tgtagagaga tacttacttt ctacattttt ttattgtata ttgctttagg
     3481 tttaaaaata tattatatac tttataatca atatatttta tctatatttt acacttataa
     3541 aatatgaaaa ctacttattg cgaaagtaat tgatttgttg tattataatt tacatgaatt
     3601 atgtaggaca atcaaaagtc agtattataa gagtacatat attcaaattt ttaacaatta
     3661 atttatataa ttaaaaaatg aagggtagga tgaagtcatg ggttttagag aagtaaaaat
     3721 tgaggaatta cagtttaatc catttactaa gattggcaaa gaatggttac ttataacagc
     3781 tggtgattca gaaaagttta acacaatgac tgctagctgg ggtggtgttg gagtctattg
     3841 gggtaaaaat gttgttacaa cttacataag accacagaga tatacaaaag aatttgtaga
     3901 tagtaatgat acatttacag ttgccttctt tgatgaaacg tatagagagg ctttaaatat
     3961 ttgtggtact atttctggta gagatataaa taaaatagaa aaagcaggac ttacaccata
     4021 ttttgtggat gatacagtag cttttgaaga agctaatatg attatagtat gtaaaaagct
     4081 atatcatggt aatatgccac cagaaaactt tgatgctaaa gaaaatgata aaaaatggta
     4141 tccagagaaa gactaccata ctatgtacat ttcagaaatt ataaaagttc ttgtaaaaga
     4201 ataattataa taaaaaatat atgaaaagaa cttattataa gtaaatgact gtgctttctt
     4261 atctgatatc taagtagatg ctagtaacta aataaatatt aattttaaat aaatactatt
     4321 ataaaaaaga tgaactatat ttactatagt tcatcttttt atttatataa gcacttatat
     4381 aagtagagat ttatttgaat aaaagtattt atttgaaata tttgagtata ctttataagt
     4441 atttttatgc taactagaaa cacttaattt atggcatttg tatttgattt attttaatgt
     4501 aaataatttc aaagttaatt tta
//
LOCUS       DAYASV010000204         4491 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYASV010000204 DAYASV010000000
VERSION     DAYASV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.209"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..2321)
                     /gene="carB"
                     /locus_tag="ACGI68_003588"
     CDS             complement(<1..2321)
                     /gene="carB"
                     /locus_tag="ACGI68_003588"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HFH0139990.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIM"
     gene            complement(2514..3563)
                     /gene="carA"
                     /locus_tag="ACGI68_003589"
     CDS             complement(2514..3563)
                     /gene="carA"
                     /locus_tag="ACGI68_003589"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFH0139991.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(3820..>4491)
                     /locus_tag="ACGI68_003590"
     CDS             complement(3820..>4491)
                     /locus_tag="ACGI68_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFH0139992.1"
                     /translation="YISKVSGVPIVDLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVP
                     VFSMSKLSKVEVSLGPEMKSTGEVLGVGENLEEALYKGFLAAGRHMSDERGVVLATVN
                     NHDKDEFIEIAKDMKELGYTFVATEGTANSLRENGIEADIVNRVEESRPNILDAIRNK
                     QVDIVINTPTKGNDSTRDGFKIRRTAIEFSTEIMTSLDTLKALVEVKKKHLNKDELKV
                     YNIAE"
BASE COUNT         1287 a          903 c          455 g         1846 t
ORIGIN      
        1 tccattatac caggtatcaa tatatcttct ttatcacata tagcatctac ttctatttct
       61 ctaccagtta gatatttatc tataagtact ggatttttat ggtctctatc aaatgcatca
      121 tccaaatact gaattagttt ttcttcgtta taagttattt ccataccttg accaccaagt
      181 acataagatg gtctaactaa tactggataa cctaactcct tagcaatttc aacaccttca
      241 ttagttgtcc atacaccttt tccttttggt ctatttatat ctagtctctc tagtaaatca
      301 tcaaattttt ctctatcttc tgctgcatct atatctctaa agtctgttcc cagtataggt
      361 atattttttt catgtaaaaa cttagccaat tttatagctg tttgtccacc aaactgtagt
      421 attacacctt ctggtttttc tttttctatt atatttaaaa cttcttcttc tgttaatggt
      481 tcaaaataca acttatcaga tgtatcaaaa tccgtactta cagtttcagg gttattgttt
      541 acaattatag tttctattcc catttttctt aaagatttta cacagtgaac tgaacaatag
      601 tcaaattcta ttccttgacc tattcttatt ggaccagaac ccaatactac aacttttctc
      661 ttatcactaa ctactacctc atcatattgt tcataagttg aatagtaata aggtgataga
      721 gcttcaaatt ctcctccaca tgtatcaacc attttataaa ctggttgtat attatataaa
      781 cttctcaatt catataattt ttctgggctt atcttcatta agtcagatat acctttatca
      841 gaaaatccct ttttctttaa ttcatgtaaa tattctttat ctaagtcttc tatttttaga
      901 acttttaact tttcttcttg ttctactatc catttaaatt tatttataaa ccacttatca
      961 acaccagtta tttctgatat catttctact ttatagcctc ttcttatcat ttcagctaag
     1021 tcaaatagtc tctcatcatc aggtactact actctagcct ttaattcttc taatgttcta
     1081 ccttctgatg gagcatgaac caatgaatat tttcctgttt caagtgatct tataccttta
     1141 agaatagcag cctcaaagtt acttcctata ctcatgattt caccagttgc catcatctta
     1201 gttcctaatt ttctattggc atgtctaaat ttatcaaatg gccactttgg tatttttact
     1261 accacataat ctaatgttgg ctcaaaacaa gcataagttt ttttagttac tgcattttct
     1321 atctcatcca aagtataacc taaagctatc ttagctgcta cttttgctat tgggtatcct
     1381 gtagcttttg atgctagggc tgatgaccta ctaactcttg ggtttatttc tattatcgca
     1441 tattctaaac tatgtgggtt taaagctatt tgaacgttac atccaccttg aacctctatt
     1501 gcatttatta tgtctattga tgcctttcta agtagttgat actcttcatc acttagagtt
     1561 tgacttggag caacaactat actgtctccc gtatgaactc caactgggtc tacattttcc
     1621 atattacata cagttataca gtttccattt ccatctctca ttacttcata ttctatttct
     1681 ttccaacctt ttatactctt ttcaagaaga acttgtccaa caggacttag ctgtagtcca
     1741 tgtgaaagta tttctcttaa ttcttcctca gtatcagcta tacctccacc agttccacct
     1801 agcgtatatg ctggtcttac aactactgga tatcctatct tattggcaaa ctcaagacct
     1861 gcttctaaat ttgtaactat atcactaact attactggtt gatttatttc tttcattact
     1921 tctctaaata aatctctatc ttcgcccttt tttatagatt ctatagatgt tcctataact
     1981 tttacattat atttatctaa tataccagca tcatgtaatt ctacagctaa gtttaatcct
     2041 gtctgaccac ccatacctgc aagtaaactg tctggtcttt ctttttctat tattttttct
     2101 ataaattcta ttgttaatgg ttctatatat attttatctg caatttcttt atcagtcatt
     2161 atagtagctg ggttactatt tactaataca acttcaattc cctcttcttt taatgcttgg
     2221 caggcctgtg ttcctgaata gtcaaactct gcagcttgac ctattattat tggccctgac
     2281 cctaatacta aagttttctt tatactatct aatttaggca taactctttc tccttatcta
     2341 aacaattttc acagttctta tcttcatcat ctatacaatc aactttaatt actttatttt
     2401 atcttaagtt caaatttact catcaataca tctatttaat acatctatgt taattaaacc
     2461 aaactttttg atttaagtta ttcttgtttt ttagttagct attctaatgt tgtttatttt
     2521 actaattcta aaaatttatc aaaaatatag tcattatctt ttggtcctgg gcaagcttca
     2581 ggatggtatt gaacactgta tacatctagc ttctcatgtc gcattccttc aacagtatta
     2641 tcatttaaat ttatatgtgt cactttcatt tgttcaggaa cttctgaaac ataataacca
     2701 tgattttgag aagttataaa tacttttcct tcttctaaat ctttaactgg atgatttcca
     2761 cctctatgcc caaatttaag tttagcagtt tttcctccaa gagctaaagc taaaagttga
     2821 tgtcctaagc atattccaac tataggtttt ttaccaataa gagcttttat attttctata
     2881 acatcttcta agtcttctgg gtctccaggt ccatttgaca agaatattaa gtcagggttt
     2941 atactcaata catcttctgg agatgttgta gctggaaata ttgttatatc acatccacga
     3001 gctataaatg atcttaatat atttcttttt actccaaaat ccataacagc cacttttgga
     3061 ccatttcctt ttatatgctc tacttctttt cttgtaacag ttctaactgc ttctgtattt
     3121 gaaaacttat ccaatttatg tttaacatct tctaatttac tgtcttctaa tgtaattata
     3181 cctttcattg ttccattatt tctaagtatc ttagtcaaag ctcttgtatc tattccttca
     3241 agtccgataa ctttattttg ctttaaatat tggtctatat caatttcaca tctaaaatta
     3301 cttggagaat cacttttctc ccttactatg aatcccttta cttgaacttt ttcagactct
     3361 gctgatgata aatttatacc ataatttcct acaagaggat aagtcatagt aactatttga
     3421 ccatagtaag aagggtctgt taaaacttca ccatatccta tcatcgaagt attaaatact
     3481 acttctccta cactttcttc taaatatcca aatgcctttc ctataaaaac tgttccatct
     3541 tctaatatta accttgcttt catactacag tacctcctat aattctcttt agacaaacta
     3601 aagaacatta actaaatata agcttttaaa tatctatatc tatttttcac ttttacattc
     3661 tagttttata cttatttaca tttatttttt atctttttct attatactac aaatttctta
     3721 aaaaaaagaa gttatctcaa aagtaaaaac tcctagaaaa aataacctct tttttttatt
     3781 taataattaa attcttaaac tatacaatca acttaatttc tattcagcta tattgtaaac
     3841 ttttaactca tctttattta aatgtttctt cttaacttct actaaagctt ttagtgtatc
     3901 caaagatgtc attatttcag tagaaaattc aatagctgtt cttcttatct taaatccatc
     3961 tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct
     4021 tatagcatct aatatgtttg gtctagattc ttctactcta tttactatat cagcctctat
     4081 tccattttct cttaagctat tagctgttcc ttctgtagca acaaaagtat atcctaattc
     4141 tttcatatct tttgctatct ctataaactc atctttatca tgattattta cagtagcaag
     4201 tactactcct ctttcatcag acatatgtct acctgctgcc aagaaccctt tatataaggc
     4261 ttcttctaaa ttttctccta cacctagaac ttctcctgta gacttcattt caggtcctag
     4321 acttacttca actttagata atttagacat tgagaatact ggtactttta ctgatactaa
     4381 ttttggctct ttgtaaactc ctgtaccata tcctaaatcc tttaatttag cccctaacat
     4441 acacttagta gccaagtcaa ctattggtac tccacttact ttacttatat a
//
LOCUS       DAYASV010000205         4454 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYASV010000205 DAYASV010000000
VERSION     DAYASV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.210"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            89..388
                     /locus_tag="ACGI68_003591"
     CDS             89..388
                     /locus_tag="ACGI68_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0139993.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
     gene            712..2367
                     /gene="ilvD"
                     /locus_tag="ACGI68_003592"
     CDS             712..2367
                     /gene="ilvD"
                     /locus_tag="ACGI68_003592"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFH0139994.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2711..3340
                     /locus_tag="ACGI68_003593"
     CDS             2711..3340
                     /locus_tag="ACGI68_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0139995.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFKQ"
     gene            3366..4178
                     /locus_tag="ACGI68_003594"
     CDS             3366..4178
                     /locus_tag="ACGI68_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0139996.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFTKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLVEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1729 a          497 c          808 g         1420 t
ORIGIN      
        1 tagggatata aaaattttat cgtatgcata aatattataa atatatggaa ataataatag
       61 tatattttgg taaagtagga ggaattttat gaaatggatt aaatggtata gtattacatg
      121 catatgtatt tttatagtag tagcttttta tatgtttata tttcctaata aaatagaaac
      181 tattgatact tcatctgcat atagctttgt tgaaaagaaa gtaccaaata gtgctgtgta
      241 tcaaggatac aaaaagaatc cagttgatgg tactactaca atttattaca gctatgataa
      301 ttcaactcat atagttagat tatcacatcc agaagactat tcaaggaaga ttaattggga
      361 taaagtttca aacatcagat ttgattaatc tttaatattt ataaaagtct atatatctaa
      421 ggcaaatata ttattaatct tattcatagt tgagcacttt ctattataga agtgcctctt
      481 ctgatttata tcataaaata ttactcaaca atttgtaaaa tatattagtt tataaaatat
      541 attgaaaaga taatattata taatacgatt gtgtaagata ttgataaaaa atcgtaataa
      601 attttcatta gtatatgcta atattttttt acaattttcg taattaatta atagaaaaat
      661 atattataat aatattatga aaatttaaaa aagtaagaaa gaggtaatat tatgagaagt
      721 gatataaaaa aaggaataga aggagctcca aagagagcgt taatgtatgg aatgggtctt
      781 actaaagaag aaattgaaag accattaata ggtatagtaa atgcacaaaa tgaggtgatt
      841 cctggtcatc tgcatcttga tgaaatagca gaagctgcaa aaaatggagt aagaatgagt
      901 ggtggtttgc cattagaatt tcctgctata ggggtatgtg atggtatagc tatggggcat
      961 gtcggaatga attattccct ggcttcaaga gaattaatag cagattcaat agaagctatg
     1021 gctatggcac atggttttga tgctttggta cttataccta attgtgacaa gatagtgcca
     1081 ggtatgttaa tggctgctgc aagacttaat ataccaagta ttgttgtgag tggagggcct
     1141 atgcttccag gtaaaaaaaa tggaaaagta tatgatttta actctgcaat ggagggtgtt
     1201 ggagcatgta aagatggaac agtaagtgaa gaagaattag aagagttagc catgaactct
     1261 tgtcctggat gtggttcatg ttctggtctt ttcacagcaa atagcatgaa ttgtttaaca
     1321 gaagctttag gcatgggtat accatataat ggtactgctg cgtcacattc tggagaaaga
     1381 aaaaggatag caaaatatgc aggtatgtat gttatggagt tacttaagaa cgacataaaa
     1441 cctagagata ttttaacaat agatgctttt aaaaatgcta tagctgtgga tatggcaatg
     1501 gctggttcta caaatacagt acttcactta cctgcaatag cttatgaatc aggaatagag
     1561 cttaacttag atttttttga tgaaataagt gaaaaaactc cttgtttaac aaaattaagt
     1621 ccaagtggaa aacatcatat tgaagattta catatggcag gaggaatacc agctataatg
     1681 aacgaacttt caaagataaa tggaataaat ttagattgca aaaccgtaac aggcaagact
     1741 ataggggaaa atataagaaa ttgtgaaata gaaaatgaag aagtaataca tacattaaag
     1801 aatccatata gtaaccaagg tgggcttgca atattgaaag gaaatcttgc tctaaatgga
     1861 gctgttgtaa aaaagtcagc agttgcagaa gaaatgttag ttcatgaagg accagcaaga
     1921 gtttttaatt cagaagaaga agctgtaaat gctatttttg gtaaaaaaat aaacaaaggt
     1981 gatgttatag ttataagata tgaaggtcca aagggtggtc caggaatgaa agaaatgcta
     2041 tctcctacat cagcagttgc aggaatggga cttgataagc atgtagcact tcttactgat
     2101 ggtcgttttt caggggcaac tagaggggca tccataggcc atatttctcc agaagctatg
     2161 gaaggtggtt taatcggact agttgaagaa ggagatataa tttctataaa cataccagac
     2221 aaaaaattag agttaaaagt agatgaagtt gaaatagaaa atagaaaatt aaaatttaaa
     2281 cctttagaac cgaaaataaa gcatggatac ttaagtagat atgctaaact ggtaacatca
     2341 gcaaatacag gagcagtttt aaaatagtag actatattta tgtaaattag aaaaagttaa
     2401 gaaaatatta attggatatc ttaggtgtta tagcactaat gaggtatcct ttttattttg
     2461 tatgaaaaac atagcaaaat cttgattatc tttgaaaaca atacatctct attataagga
     2521 atacgagtac agcatagaga tgtatctttt agaccttgtt gaggtaatac aactttttaa
     2581 aagtttgatg tggaatgctt ttcgttcctt ctgaactatg aagaaattga aagtaagtta
     2641 attcttgaca taataaatat taaaatgtat aataaaaaac aaacagacag tctgttttta
     2701 ggagggaaat atgaatagag aagaaaaaag taaaaatagt aaagaaaaaa ttatccaatc
     2761 agcattttca ctattttcgt ctaaaggata tgattcaaca tctacacaag atattatcaa
     2821 tttatctggt ctatctagag gtgcaatgta tcatcacttt aaaactaaag aagatatact
     2881 gagaagtgtc acaaaagaac tttactcaca aatgaataat tttttagagc atcttgttgc
     2941 tgatgacacc cttacagcaa atgaaaaaat aatagaattg gttgttcata gtgcgaatga
     3001 ttacacacgt agaaaaatgg tacattgtag ctggttagaa aaaatcccat tcgctttaat
     3061 agaggaagtt cgtaatctca acaatgtagt tgcacccaat attgctaaga taattaaaca
     3121 aggtgttgaa aataaagaat tttcttgtga atatccagag gaattagctg aaatgctcgt
     3181 ttttagtatt gatattttac ttgaccctgt attatttaaa cgtgaataca gtgaagtatg
     3241 taataggttg gattttttat tattcatgtt aaaaaaaatg gatattcctc taattgatga
     3301 gtatggtatt caaaaattta aagatttatt taaacaatga taataggaaa agtgaggaat
     3361 agactatgtt ttataatgca aaaagttgtc aaatgatgat aggaagtaat acagtgaatt
     3421 atattgaatt tggtagtggt aagaagacac taattatttt accaggatta ggagatggtt
     3481 tatttccttt acatggaaaa atacaggcga ttgcttttgc ttttagatat aagcaattta
     3541 caaaagatta taaggtttat gtatttagta gaaaaaatca aattacagaa aaatattcca
     3601 caagggatat ggctaaagac caagcagata taatgaaaaa acttggaatt atgaaagcag
     3661 aagtaatggg tgtttctcaa ggtggaatga tagctcaata tctggcaatt gattatcctg
     3721 agttagttga gaaacttgtg ttggcagtta cttcttcaaa gcaaaatgat actatccaaa
     3781 atgttatttg tagttggatt gatatggcga agaagcagaa ttacaatgat ttaatgattg
     3841 acacagcaaa gaaatcctat tctgaaagat atttaaaaaa ataccaatta tttattcctt
     3901 ttttaggaaa agtaggtaaa ccaaaggatt ttaaacgatt tattattcaa gcgacctctt
     3961 gcatagaaca taatgccttt tccgaattaa ataaaataac atgtccaacc ttaattatag
     4021 gaggtgctaa tgataaaatt gttggaaata atgcttcttt tcatttagta gaaaaaatta
     4081 aaaaaagcga aatatttatc tatgagggat tgggacacgc tacatatgaa gaagcacagg
     4141 attttaatga aagagtttta gagtttttaa ataagtaaac acctttgtta aaaatataca
     4201 tatgaagatt ggaaatttaa tgttaaaaat agaaacatga aaatatgctt aactggtatt
     4261 tttactattc ataaccaatt tttgatacat tatctactat aaatacaaat atagcttcaa
     4321 tgtgattata tattgttgta ttggtaaagc acttatacaa acagaggaat tttgtaaatt
     4381 catattatac ccacatttgt taacttatga aaatataatc aaaattttta tgagcttttt
     4441 atataaaaaa tata
//
LOCUS       DAYASV010000206         4449 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYASV010000206 DAYASV010000000
VERSION     DAYASV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.211"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(30..260)
                     /gene="dltC"
                     /locus_tag="ACGI68_003595"
     CDS             complement(30..260)
                     /gene="dltC"
                     /locus_tag="ACGI68_003595"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFH0139997.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(279..1427)
                     /gene="dltB"
                     /locus_tag="ACGI68_003596"
     CDS             complement(279..1427)
                     /gene="dltB"
                     /locus_tag="ACGI68_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFH0139998.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(1427..2941)
                     /locus_tag="ACGI68_003597"
     CDS             complement(1427..2941)
                     /locus_tag="ACGI68_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HFH0139999.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(3062..4252)
                     /gene="dltD"
                     /locus_tag="ACGI68_003598"
     CDS             complement(3062..4252)
                     /gene="dltD"
                     /locus_tag="ACGI68_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFH0140000.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
BASE COUNT         1447 a          783 c          451 g         1768 t
ORIGIN      
        1 aaattaatat ttaaagtcta actactaatt tattttcttg tctccaagaa ttttactaaa
       61 ttatttacag tagacatatc ttttctctct aaatcagttg gttgaagctc tatccctaat
      121 ctattttcaa tttcaagtaa aacttcaatt atagctaatg agtctaaaag ttccgtctca
      181 aataaattaa ggtctaaatc ttctcttatc tcatcagtcc ctaatacatc ttcaaatatt
      241 tctataactg tttcttgcat aaattttcct ccttaaaatt aagctaaata acctgagaat
      301 atcagcaatc caaaacacac aatatggaat gtaatgaaaa tctgcactct ctcgaaccat
      361 ttatcttttt tatgtttttt ataaaattta gattttcgct gatatatatc agttaacaca
      421 agtgccaatc cttggtatac accataagca acataatacc aagttagacc atgccaaaat
      481 cccattgtta tcatggttat catctgagcc acatgagcag cagtagtcct tttcttaaat
      541 cttttctttc tcattgatga cataacaaat ctagaaaaaa tataatctcc aaaccatctt
      601 gaaagactta tatgccatct tgtccagaac tctttcatat ctttactgat aaatggcttg
      661 tcaaagttta taggtacttg tattccaaat atgtatcctg tacctatagc aaataaacta
      721 tatcctgcaa aatcaaagaa caggtataaa ctatatgcgt acatataact taaaatattt
      781 ataaaactca tatctttagg tattcttgat acccaatatg tatttatcaa aaatgctata
      841 acaaacttat aacctactcc catgacaata ttttttattc ctggcagtaa gtattcttct
      901 atatactctt ttctagagat ttgtttttcc aagtcctgtt caaatcttct tgacctatca
      961 ataggtccag aacttaaagt tggaaaaaat aacataaaat aaagcatctt tgaaatttta
     1021 acttctttta tagctccatc ataaatttca ataaccattt gtatagttct gaaatttaag
     1081 tatgatattc caataaatcc aattattcca aaagaagtaa ctggagatat tttatttatt
     1141 attattggga gcattgatgc aaacaagaat ccccaatata tgtatttatt tttagttttt
     1201 cttcttacat attcatatcc tttaactatt attacttcaa gaataataaa tattaccaag
     1261 tactttagtt ggacatcaat tcctactatt aagaatatca taaacaaact tgccaacata
     1321 ccataatact ttatattctt gcccattaga cctaaaatta ctgctggtat tgatgttaat
     1381 aataatatat aaagataaaa ataatctcca tattgtgaaa aaatcattaa atttcctcca
     1441 taagtttctt tctgtctatt ttaccattga tatttgttgg aaactcagag attattgaaa
     1501 tatttcttgg aatcatatat gaaggtatat actttccaag ttcctttttt attatcatcc
     1561 catttttaat attacttaaa tcattctttt catttaactc aacaatacct tttaagtaag
     1621 caattttttc gtccttatat actggtaaaa ctactgcatt ttttacatta tgtacttttc
     1681 tcaaattatt ttctatatct tctatttcaa ttctaaatcc atttaattta atctgaaaat
     1741 cttttcttcc acaataatat atatttccat ctaaaagata tcccatatct ccagttttat
     1801 aagctctcca ctttacacca tctatttcat catagaaaaa tacttcatca gtcttttctt
     1861 tattattgaa gtaaccttta gaaactgatg gtccaattat tataatttca cccttttcat
     1921 tttctttaag ctcatttcca tcttcatcaa gtatttttat tttacaattt gacatagggt
     1981 atccaactgg aagacttttt tcatcatcta tagccttttg agtcatatca tttacactta
     2041 caccaacagt agcctcggtt ggtccatatc cattgataat tcttgtgttt ggaaatctac
     2101 tcataagttc tttagtaaga ttttttgaaa gtgcttctcc tatgaatatc attgactcaa
     2161 ggttaggaag cattttacta ttaaactctt tttcagttat acatactcct gcaaaagaag
     2221 gagttgacac ccatacagct atatctgaga tactaaattg tctaaatagt tctttatagt
     2281 ctgctaatac atcttttgat attgaaaata atgtagctcc atggataaga ccagggtata
     2341 ttgttgtaac tgaaaggtca aaagaatatg ctggttggtt cattattact ttttcacttc
     2401 catctatgtt tagatatggg cttatccaat ctgaaaagct atctaaattg tttgaactta
     2461 tttgaacccc ttttggcttt cctgtacttc ctgaagtaaa tagtatatat gcattttcat
     2521 catcttttac ccagttttct ttatctaatg attttccctg atattcattt attatataat
     2581 ttaatttatc catatcaatt acattgatat ctccaaaatc tttttcatca ctaaagttaa
     2641 ataatacctt tggttttatc tctttagtga cttcaaaaac tctatcaatt ggaaaactaa
     2701 tatcaagagg cacatacgct ctacctgatt ttaaagctcc tatcatacat gccattatca
     2761 tgttttcttt atttccataa attactatag gagtatcttc ttctttgtat acatccttta
     2821 gaaatactga tatagcatct gaatattcat ttaaatcttt ataagaaagt ttatctccat
     2881 tacacattag tgctgttcta tctgtatttg aatatttttt tattccttca ataattttca
     2941 tataagaatg ctcctttaaa atccgttata aataaatggc aattctttaa atggagtaaa
     3001 tgttagaact cctatcgcca atattataac catagttaaa caaatactag ttaacaaatc
     3061 tttattgttc tttaaatatt ttaaataatc tctcacagac atctacccat cctttcgtac
     3121 caagatgcat tacatccctt aaataatact tatctgaccc tttatcttta aggtttagaa
     3181 cttcaaagcc tttgctttct gctatttttt gtgctttatc gtagtactga tttctttctt
     3241 tttcagaaat accagtcaaa ttatagaatt tatccatact tgggattaat acaacaactg
     3301 gcttaatacc taagtcagta catacattta gcatcagttt ataattttca aactccttag
     3361 atgttaataa atttacatct ttgtctcttc ctttgtattg ctctatacca tctttaaaat
     3421 gctctttata atagtactta tcaatattta gtggattttt accaactctt tttttagcat
     3481 cttcaatggc tttctttctc tcatatgacc aatttattgg tctttttctt tttgttgctc
     3541 tctttttatc tagcttaatt aatcgtttat aaagaatacc cttttcttta agagctatac
     3601 aatactttct tccttgaaaa taaggctcta aaagcacctt ttcagctttt tcaggaaatg
     3661 tcttaggctc atatagctta gcatataatg cttctgcttt atactcgtca cttccccata
     3721 ataacttact tgattttttt gcatattcaa tcttattttg tttagatatt tttggattat
     3781 ctaaaaatcg ataaaattgt attggtgaaa atctagactg ataatggtga cttgtgactc
     3841 cgtctttttc cataaaccat tgcatagaaa ttaacaagac aactttctta ttatcaatat
     3901 taggattcat acttcctaat attgcagcat cttgtaatgt ctgagtatag gctctcccaa
     3961 ttgcaaacac cttattttta ctcctatttg tattaaaata gtactttgga tgttgttttg
     4021 ttgaattact aagttctgat gaccccaaaa tcataatatc cttttctctt aaaaaatgat
     4081 tatttgctgt aaccccttta tcttttatat cacttaaagt atcatccata attggaagta
     4141 gatttttctc cctcaaaagt tcatcagttt tagaatcaag aaatttgtct aacccaaata
     4201 aaaatactac gcctattata aaaggtgtaa taaagtatat caattttctc atattttctc
     4261 tcctctaaaa atattcaaat ttatgttatt attaatcata ttagttttga tatgtatatt
     4321 caactacaaa attgtaataa aatcgtcaaa actttttttg atactgttac aaaattgtaa
     4381 aaaaaaagaa aaaaacgcca aaaataaatt tctttttagc actttttcat acttataatt
     4441 tgacatttt
//
LOCUS       DAYASV010000207         4435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYASV010000207 DAYASV010000000
VERSION     DAYASV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.212"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            171..956
                     /locus_tag="ACGI68_003599"
     CDS             171..956
                     /locus_tag="ACGI68_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0140001.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            1235..3145
                     /gene="phnW"
                     /locus_tag="ACGI68_003600"
     CDS             1235..3145
                     /gene="phnW"
                     /locus_tag="ACGI68_003600"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFH0140002.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            3504..4061
                     /locus_tag="ACGI68_003601"
     CDS             3504..4061
                     /locus_tag="ACGI68_003601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140003.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHISSR"
     gene            4154..4366
                     /locus_tag="ACGI68_003602"
     CDS             4154..4366
                     /locus_tag="ACGI68_003602"
                     /inference="COORDINATES: protein motif:HMM:NF024570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4004 family protein"
                     /protein_id="HFH0140004.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLD"
BASE COUNT         1830 a          421 c          820 g         1364 t
ORIGIN      
        1 aaagtccatc tgatactaat atttgatgga cttttttaat gataatacaa agtcttttga
       61 aaataacttt aattaagtaa aaaatttaat acataaatat gatttcataa atcttaggta
      121 tcattgtaat attataggag aaaaatatat tatatttagg aggaaaatga atgtctttaa
      181 attcgataga tagaaaaaaa gaaatactta aattattaga ttcaaaaggg aaggtaaaca
      241 ctaaagaatt agtaaaaaga ttagaagtat catcagaaac aataagaaga tatttggaag
      301 aattagaaaa tgaaaataaa ttaaaaaagg tatatggtgg tgctataaaa ataaattatg
      361 gagtgattga accagaacat ataaagagaa acaatactaa cctagataaa aagcaaagta
      421 ttgctaaata tgcagctaag tttgtaaatg acaatgagtc aattatcata gatgaaggta
      481 ctaccaattt gcagattgta gattatataa tatctgtaaa caatttaaaa attattacaa
      541 actcttatcc agttctatca aaattgattt cttatgagaa tcaaggtttg tttgatggag
      601 aagttatatt tattggtgga aaagtaaatt taaaacacca aaggacagca tgtccaatgt
      661 caatagatac tatgaaaaac ttatacgtag ataaagcttt tatatctagt gaaggtatac
      721 ttgatagttt tggaattagt agtatagacc aaaataaggc attgttgtca aaagaatata
      781 taaaaaattc aaaggaaagt tttgtattat gtgatagttc taaaatagga attgctagta
      841 tgtataagat agcagattta gaggaattag attttgttat ttgtgatgta gaacctccga
      901 atgaatggaa ggataagtta aatgagataa atataacatg gatagaagca aaataaaccc
      961 ttagttttat ataatacaac tatgctcaat acaactaaat tcataaaata tagtcagttc
     1021 atagattaag attagaaagt aaattttaga ttgtaactta ggattttatg ggaatatgat
     1081 acaaaataaa aacaactaaa tacaactaaa aacagaaatt taatatatat ttaacctaaa
     1141 tatgatgtat aattaacata tgaaagttat actttaaaag taaaaacaat taaaaacaca
     1201 aaaattctaa aaagaatcta tggaggctat aaatatgaaa aagatatatg gagaaaaaat
     1261 aaaagcagta gtattcgatt gggctggaac aacagtagat tatggatgtt ttgcacctct
     1321 taatgtattt atagagatat ttaaaagaag aggtatagat gtaacaatgg aagaagcaag
     1381 aaaacctatg ggaaaattaa aaatagacca tataagagaa atgtgtgaaa tggatagaat
     1441 aaagaatatt tggtcagata agtttggtaa agtgccaact gaagatgatg taaatgaact
     1501 ttatgctgaa tttgagccaa tgttatttga gacattagaa gagtatacga ctccaattcc
     1561 tcatgttgtt gaaactattg aaaagttgag aaaaaatgga ttaaaaatag gttctacaac
     1621 gggttataca agagagatga tgaacatagt tgaaccaaat gcagcaaaga aaggatactc
     1681 acccgatttt cttgtaacac catcagaagt atctcaagga agaccgtatc cttggatgtg
     1741 ctataaaaat gctgaagctt taggggtgtc tcctatgagt tctatggtaa aagtaggaga
     1801 tacaataagt gatgtaaaag aaggcgtaaa tgcaggaatg tggagtgttg ctgtaataaa
     1861 aggttcaagt gagttagggc ttacacaaga agaagtagag aacatggata aggaagaatt
     1921 aaaagctaag atgagtatag tttcaaagaa atttaaagag gcaggtgcac attttgtaat
     1981 agaaactatg gctgagttag aagatatact aataaaaata gagaatgaaa ctattaaatc
     2041 agattttgta ccagaaaatg attacatatt acttacacca ggaccactat ctactactaa
     2101 atcagtaaga gcatctatgt taaaagattg gtgtacttgg gatgttgaat ataataacct
     2161 agtacaagat gttagaagaa gattagtatc tctagcaacc caaaatacag ataaatacac
     2221 aagtgtatta atgcaaggaa gtgggacttt ttcagtagaa gctataatag gaagtactat
     2281 ttcaaaagat ggtaaacttc ttgtaattgc aaatggagca tatggaaaga gaatgaaaga
     2341 tatatgtaat tatcttaaca tacaatttgt tgactgcaca tttaaagata tagaagcagt
     2401 ggatttaaat gtagtagaaa atttattaaa agaaaacaag gatataacac atatatcaat
     2461 ggttcactgt gaaactacta caggtagatt aaatcctata caagaggtag ggaaacttgc
     2521 aaaagaatat aacaaaattt atatagttga tgctatgagt agttttgggg gaattgagat
     2581 tgatgttgaa gattttaata tagatttttt agttagtagt tctaacaaat gtatacaagg
     2641 agtacctgga tttggtttta taatagctaa taaagagaaa ttatctaaat gtaaaggtat
     2701 agctaaatca ttatctcttg atgtgtatgc gcaatgggag actatggaga aaaataatgg
     2761 taaatggaga tttacttcac ctacacatgt tgtaagagct ttttatcaag ctttattaga
     2821 attggaagaa gaaggttctg ttgaaaaaag atatgctaga tataaagaaa atcagtttac
     2881 aatagcttca agattaaaat ctttaggctt tgatactttg gtgaatgata atgctcaatc
     2941 acctgtaata acaactttct tatatcctaa aaatgcaaag tttgaattta tggaatttta
     3001 tacttattta aaagataatg gatttgttat ctatccaggt aaattgactg atatagatac
     3061 atttagaata ggtagcatag gtgaagtcta tccaactgat atggagagac tagctgatgt
     3121 aatagaaaaa tttatcaata gatagaagaa gatagtgtct tgtaggttac agaatagtgc
     3181 gcaaagccta ctcctttagg tgtgtgagat gatagcacaa acagtgtata aaaagtattg
     3241 acagtagaat ttaaagactt atattttaga agtttataca atgaatatat aaacattgag
     3301 taatagggat atattttatt taaaccacaa tctaagaaat tagatttata gtttaaataa
     3361 agcatttccc tattattttt atgcctattt atagaaaata aatgatagtt gctttattaa
     3421 gtattattat catacttgac aacgtaacaa tgttttgtta tattaggttt aaagataaaa
     3481 tacggtaaag aaaggatatc attatgaatg aagaattaag ttcactaaga tttgatggtg
     3541 ttggaaaatg taaaggtgga atttttgata atgttaatat atcaggaaca ggcaaaatag
     3601 aaggaagtgt aaaatgtagt aaatttgatt cttctggttc agcaaaagtt tttgggacaa
     3661 cagaatgtaa tgaatttttt acatctggtt taagtaaagt agaaggaaac atactggcat
     3721 caaaagttga gatatcggga cttcttagat gtacaggaaa tattaattct gttgaaattg
     3781 atactagtgg aattattaat gtagaaggaa gtataaaatc ggatattatt catggagaag
     3841 gatatttaaa agcaaatgag aatatagatt gtgaaaatat caatttaagt ggaatcttgg
     3901 agtgtaatgg atttttgaat tgtgaagaag ttaatattag tttaaatgga acttctaatt
     3961 ttaatgaagt tggagcaagt agtataaata taaaaaaggg gcatggagat tattgcaaaa
     4021 aatttcgtat gctagaaaaa aagcatatct ctagtagata attatgatgt tggaaatatt
     4081 gaatacagtg ataatttatc agtttcagat aattctactg taaaaaacag tataaaggtg
     4141 ggctagaaga gctatggaag aattaatatc taagaaagat ttattgagca aaactaatat
     4201 atcttatgga caattatata gatggaaaag aaaaaacata ataccagagg aatggtttat
     4261 aaaaaaatca gcttttacag gtcaagagac tttctttcca agagataaaa tactagaaag
     4321 aatagagtta atattgtcta tgaaagaaga gacttctctt gattaaaaaa tagctatgca
     4381 cacttgtgac tttagtcatg agttagtagc taaaatagtc agcatatagg gaaac
//
LOCUS       DAYASV010000208         4385 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYASV010000208 DAYASV010000000
VERSION     DAYASV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.213"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            721..1629
                     /gene="trxB"
                     /locus_tag="ACGI68_003603"
     CDS             721..1629
                     /gene="trxB"
                     /locus_tag="ACGI68_003603"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFH0140005.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCITSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1719..3542)
                     /gene="typA"
                     /locus_tag="ACGI68_003604"
     CDS             complement(1719..3542)
                     /gene="typA"
                     /locus_tag="ACGI68_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFH0140006.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3754..3996
                     /locus_tag="ACGI68_003605"
     CDS             3754..3996
                     /locus_tag="ACGI68_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140007.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            4144..4335
                     /locus_tag="ACGI68_003606"
     CDS             4144..4335
                     /locus_tag="ACGI68_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HFH0140008.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1623 a          556 c          691 g         1515 t
ORIGIN      
        1 tttaaattgt aaaaaaattg taatcaaatt gagtattaga aaaatatttt tttaactttt
       61 ttaaaagttt aaaaacctag attttcatag tgatgaagct gtttttaatg aaagtaatta
      121 aaagataact attaaatatc tgtaatatta caattttata ctacactata ttacaaaaaa
      181 agtttacaaa actttaattt gaaaaatgta attatatggt atatcatata aatataagta
      241 aatagtgcta atatttaaac tattaatcat aattggttca acaaaagtat atagttaata
      301 atgtttaatt tgaagaaatt gttagtgtga aattacatat atattcaaat taaattcata
      361 aatatttcac ataataatag tatactgtaa acaaaaggag gtattaaata tgggaatagt
      421 acttataaca gcagcattag ttttggttat gtatggactt tcaattctat catatatatc
      481 agaaagcaat atattagaag acactgtaaa tgataaccaa taaggttttg aattatataa
      541 ataagtgaga actgtaaata aaagttaatt tttatttgca gtttttttat atatatgtaa
      601 aataaagtta attaataaaa aataatgttt tgtttatagt atatatggat aataatagta
      661 aagtataaga atgatgagaa taatatataa gaaataataa tataaagaga ggtaaacgtt
      721 atggtagata tcattgtaat tggtgctgga ccagcaggtt taacttcagc aatttatgct
      781 atgagagcag ggttaagcgt tacagtattt gaaaaaaata tttatggagg tcaagttgca
      841 agtacctctg aggtagaaaa ttatccagca gttcaaaaga tttctggggt agaattttct
      901 aataatatat acaatcaagc agttgctcaa ggtgttgata ttcagtttga tgaggttgaa
      961 gagataaatt tagagggaaa agtaaaggtt gtaaaaactt cttcaggtga acataaagca
     1021 aaggctgtta ttcttgcaaa tggagttgaa agacgtaaat taggttgtgc aggggaacaa
     1081 gaatttacag gtagaggagt atcttactgt gcaacatgtg atggagcctt ttttaaagat
     1141 aaagaagttg ctatagtagg tggaggaaat actgcactag aagatgcttt atttttagct
     1201 aataattgta caaaagtgta tttaattcac agaagagata gttttagagg tgaagaagtg
     1261 ttggagaaat ctgtgaaagc aagagagaat attgagatac tttacagtca tggtgtagaa
     1321 aaaatagaag gagaaaagac tgtatcgaaa atagaagtaa aaaacttaaa gactgaagaa
     1381 aaaagaacta tagatgttag tggtattttt attgctatag gtcttaaacc taataataaa
     1441 atgtttgaaa atgttcttga tttggatgaa ggtggatata taatttcaga tgagtcttgt
     1501 ataacatcag tagaaggtgt atatgtagca ggagatagta gaactaagtt tttacgtcaa
     1561 ataataacag cagcatcaga tggagctatt gcagccgttc aagcagctaa ttatataaat
     1621 gttgaataat ataaatttca aataaaaata gccatataaa ctgaaataat ttttcagtac
     1681 tatatggcta ttttgttaat tagaggtaaa tgtacgtttt atttaccttg tctagatttg
     1741 ttatatctta atctatcatg ttcatttaag aatctttttc taagtcttat agaatcaggt
     1801 gtaatttcaa ctaattcatc atcttctata aactctagtg cttcttcaag agtaaatatt
     1861 ctaggaggag ataattttat agcatcatct gaaccagaag cacgtacgtt actcattttc
     1921 ttgtttttac aagggttgac taccatatca tcttttctgg aattcatacc aattatcata
     1981 ccttcgtaaa cttctacacc agggtctaca aacattacag ctctatcact taaggcatta
     2041 agagaatacc ccatagtaac accaggtcct tgtgctatta aaacaccatt tcctcttgaa
     2101 ggtatttctc ctttaaattc ttcaaatttt tcaaatgaac gaactaaagt accttctcca
     2161 cgagtagcgt ttataaattc acttcgatat cctaaaagac cacgagtagg tgctaagaac
     2221 tctattttta catagtctcc ttctattgac atagactcca tcataccttt tctcatgttt
     2281 aattcattta taatagtacc agagtaaact tcaggacagt ttacaacaac tctttctatt
     2341 ggttccatta attttccatc ttctttatgc attaaaactt caggtttaga aacaccaact
     2401 tcataacctt cacgtctcat attttcaagt agtatagata agtgaagctc tccacgtcca
     2461 gaaactttgt atccatcagt agtatcaaga ggttcaactt taagaccaac atttacttca
     2521 agttcctttt caagtctatc tttcaaatgt ctagttgtaa cgaattttcc actttttcca
     2581 acgaatggag agtcgtttac taagaagttc atagaaagag taggctcttc tatttttatc
     2641 atttccatag gtagtggact atctaaatcg catatagttt cacctataga tatatcagga
     2701 ataccagcta taacaactat ttctccacta gtagcttcat caacttcaac ttgttttaaa
     2761 ccttcataca cagaaagttt tgatactttt cctttagata caacagaatc ttctctacat
     2821 atagcaactt gtgtattgtt tttaagagta cctttgtaaa ttctacctat accaagtcta
     2881 cctacataat catcatatgc aagagctgat atttggaact gtagaggttc attatcgtaa
     2941 ttaggataag cttcaacgtg atttacaata gttttaaata gaggagaaag gtcttcacta
     3001 tcatcatcca tttcaagttt agcaatacct tgtttagcaa taccatatat tataggaaat
     3061 tcgcattgct catctgttgc atttaagtct acaaataaat cgaaaacttc attaactact
     3121 tcttccgctc tctggtcttt tttatcaatc ttgtttataa ataatattgg ttttaaaccg
     3181 aactctaaag atttttgtaa aacgaatcta gtttgaggca ttgggccttc actagcatca
     3241 actaatagaa taactgtgtc aacagttttc ataacacgtt caacttcaga agaaaagtca
     3301 ctgtgtcctg gagtatcaac tatgtttatt ttgtaatctt catagtttat tgcacagttt
     3361 tttgaatata tagttatgcc tctttctttt tcaaggtcgt tactgtccat aacgcagtct
     3421 ttaactactt cgttctttct aaaaacaccg ctttgagaaa ggaaagcatc aaccaatgtt
     3481 gattttccag cgtcaacgtg ggctataact gcgatattta taattttatg tttttgtgac
     3541 atttttgttg ctcccttcga ttaaaataac ttattaatta tagcacaata tattattagg
     3601 tacaaggagg aatatattaa attgtattaa tatagtttaa attgaataaa actgttagtt
     3661 ttgcatctga aaattatttt gtttaaaaaa attttttaga caaataataa ccttaataga
     3721 taaaaagatt aataaaagag taaaggatga tatatgttgg aagtaaccaa aataataatt
     3781 aaatattttg caagtgaaag tgatactttt gtaaaagaag aagtttgtaa ttttgaagaa
     3841 cttcttaagt ggtctcataa atttattgaa ggtaaaaaga tagaagatgt tttgtatata
     3901 aagtctaaag aaaactataa agtaaatgtg attaagtctt tagaaattaa ggaattggaa
     3961 atagtgagaa aacaacagat accaccgtac atgtagtatt aataatatat aaaattaata
     4021 tgtaatactc gcaataggat ataaatggta tatatggtaa taatttatat taaaaaaact
     4081 tttataaagt ttatatttgg gtataaaagt attaattaga cattaattag gaggaactta
     4141 aatatgataa gagtatgtcc aaactgttca aatgtggatg taaataagct aaaaactttg
     4201 gttggagaag aaaatgtaaa gactggttgt ataggtcagt gtagagcatt taaaaaagaa
     4261 gctgttggaa taatagacaa tgagcttgaa ataaaggaaa cagaagcaga gttttttgaa
     4321 gcatgtaaag aataaatttt aaataaaata taataaatag agcatctaaa ttgtataagc
     4381 attaa
//
LOCUS       DAYASV010000209         4326 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYASV010000209 DAYASV010000000
VERSION     DAYASV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.214"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            432..635
                     /locus_tag="ACGI68_003607"
     CDS             432..635
                     /locus_tag="ACGI68_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140009.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            799..1953
                     /locus_tag="ACGI68_003608"
     CDS             799..1953
                     /locus_tag="ACGI68_003608"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFH0140010.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            1966..2970
                     /gene="rseP"
                     /locus_tag="ACGI68_003609"
     CDS             1966..2970
                     /gene="rseP"
                     /locus_tag="ACGI68_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFH0140011.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            3153..4208
                     /gene="ispG"
                     /locus_tag="ACGI68_003610"
                     /gene_synonym="gcpE"
     CDS             3153..4208
                     /gene="ispG"
                     /locus_tag="ACGI68_003610"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFH0140012.1"
                     /translation="MSYERRKTREVSVGAVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
BASE COUNT         1678 a          432 c          806 g         1410 t
ORIGIN      
        1 attcagtatt tctaataata agattctttt tgtatttatt taatatgatt ttatcaagaa
       61 taaaagatta aactaatttt aattatttta attgttcatt ctaaaatagt atagttctaa
      121 ctttctatag tatccatcat agcccttgtc tcttattata atcaagtact ttaaatatat
      181 aataaaatct ttcttatata tctgcaaata taataatagt atattacttt aatttagcac
      241 caataaaaat ttttatatgt taataataat gcatcatatg tgttatatac caatcattta
      301 gtatagtatt aaattttcat attagaagga taaaattatt tgacacttaa gcaggtgatt
      361 atttatctaa atagatgaaa attttccaaa atagataaaa tgtgatagaa tatattttgc
      421 gaggtgataa catgatttca tacgctccat tatggaaatt gttaattgac agaaaaataa
      481 agaaaatgga atttgtaaat atatcaggta taagtatttc tgttttaggc agactaggaa
      541 atgataaatc tgtttctatg gatacaatgg agaagatatg tttagcacta gactgtaaaa
      601 tagaagatgt tgtggaaata aaaaagggca tttaataata ctagaatatt ttataatatt
      661 ccacaaaata caaaaaataa tagattaatt ttaatctgtg gaataatata tgaaatttct
      721 atgaatagta aaaataattg ttttagtagt gttataatgt tttgacaggg tatatttatt
      781 attagaaagg attattatat gaaaaagata tcaattttag gttctacagg ctctataggt
      841 aaacaaactt tggatgtggt tagagaacat agagataagt ttgagatagt tgcaatatca
      901 gctaacagta atattgaatt attgctagaa cagatagtag agtttaaacc taaatatgta
      961 acagtttttg aggaaaataa agcattaaaa ttaaaagaaa tgttgcctaa aaatatagaa
     1021 atagaagttt tagcaggaat ggaaggatta aaaataatat catcacttga tgaagtggat
     1081 gtccttttaa ctgctgtagt aggtatgata ggattagtcc caactctttg tgctataaag
     1141 aagggaatag acatagcatt agcgaataaa gaaacgttgg taactgctgg tgaacttgta
     1201 atgaaagaag cagaaaaata caatgtaaat attcttcctg ttgatagtga acatagtgct
     1261 atatttcaat gtctaaatgg agaaaataaa aaaaatatag aaaagataat acttacagca
     1321 tctggtggtc catttagagg aaagaaaaag ggagaacttg caaatataac taaaaatgaa
     1381 gctttgaagc atccaaattg gagtatggga agaaaaataa gtattgattc ttcaacgctt
     1441 atgaataaag gacttgaagt gatagaagct agatggttat ttggagtaga acaagaaaat
     1501 atagatgtag tagttcaccc acaaagcata attcattcaa tggtgcagta tacagatagc
     1561 tcaataattg ctcaattagg atgtccagat atgagactac caatacagta tgctcttaca
     1621 tatccagata gaatggaaag tagctttgaa agaatgaatt tttcaaaatt tagtacttta
     1681 acttttgaag aaccagattt agaaactttt ccatgtttaa aattggcata tgagtgttta
     1741 aaaatgggag gaacttattc ttctgtttta aattcagcaa atgaagtttt agtaagtgaa
     1801 tttttagagg ataaaattgg tttctatgac ataccatatt acatagagaa gactttagaa
     1861 gttcatagta gcataagtga accaacatta gaagaaatct tagaaacaga tagatggagt
     1921 agagcttatg ttgcaaatct gataaaaaaa taggaaggtg aatttatgac tataatagcc
     1981 gcattaatac tatttagtat aattgtatta atacatgagt taggacattt tatatttgca
     2041 aaaagaagtg gtatcaaagt aaatgagttt tctataggta tggggcctaa gatatatagt
     2101 gtaaaaaaag atacagagta ttctataaga gcacttccaa ttggtggata tgtcagcatg
     2161 gaaggtgaag atgaagaaca aataagtcca aattcttttg gaaacaagtc tatattacaa
     2221 agatttagta ctattgtagc aggaccaata ttcaatataa tattagcagc aatactttta
     2281 gttccagtat ttttgtatat aggttctcca acgactaaat taggaaagat aatgccagat
     2341 actcctgcac aggctgtagg gcttcaagta ggagataaga taaataaaat taatggtaat
     2401 tctgttaaga cttgggatga agttgcaaat ataataaaca cttcttctgg tggagaatta
     2461 aaacttagta taactagaga tggaagtgat aaagttgtaa atgtaacacc taaaaataac
     2521 aatggaaagt atgaaatagg aattcaacca caaagagaaa aagacttttt agggtcaata
     2581 gtaaatgcat gtaaaactac tgtagatatg acaaaacaga tgctaacatt tctaggtcaa
     2641 atgattacag gacgtgtacc aggagggatt ggcaatgctg tagcaggacc agtaggtgtt
     2701 attggtatgg tatcagatgc tgctcgtaca ggcatcataa atgtagtgta tttagcagct
     2761 gtcataagct taaatttagg tatagtaaat ctattaccaa taccagcact tgatggatgg
     2821 agaatactta tgttgttact agaagctgtt agaggtggta aaaaactcga cccaaataaa
     2881 gaaggcatga taaatgttgt tggttttgga gctttaatgt tatttatgct ttttatcaca
     2941 tataaagata tactaagatt atttcaatga tttagttttt tagagatttt aagaaaatag
     3001 gctatgtagt tttaaataag aataaattaa tttttatata tgaaatttat tcttatttaa
     3061 ctagatagct ttttaattta taatatacat gtaggttata atattttaat atttaaagag
     3121 attcaatata aaataatata tagaggtgga atatgagtta cgagagaaga aagacgagag
     3181 aagtaagtgt aggagctgta aaaataggag gagaaaatcc tataagtata caatctatga
     3241 caaatactga tacaagagat gcagatgcta caatagctca aataaagaga ttagaggaag
     3301 ctggttgtga tatagttaga gttgctgttc ctgatattaa agctgctaaa aatatagcaa
     3361 agataaagtc tagtgtaaat ataccaatta ttgcagatat acatttcgat tataagctag
     3421 cccttgaagc tatagaacaa ggtgtggatg gaataagaat aaatcctggt aatattggaa
     3481 gtatagagag agttaagatg gtagttgaga aatgcaagga aagaaattta aaaattagaa
     3541 ttggagttaa tggaggttct ttagagaaag aacttttaaa aaagtatggc tctgcaactg
     3601 ctgaagcatt agttgaaagt gcaatgggac atattaagat tcttgaagac ttagattttc
     3661 ataatatagt tatatctcta aaatcatccg atatttataa aactgttgat gcatatgaat
     3721 taatatcaaa aaaagttgat tatcctcttc atattggaat tacagaatct ggaagtgttc
     3781 acaaaggaac aataaagtct tctatagggg taggagcact tcttcttaaa ggaattggag
     3841 atactgttag aatatcttta acaggcgacc ctgttgaaga ggttattgtt ggtaaacaga
     3901 ttttaagaag tttgggactt ttaaatgata agattaaagt tatatcatgc cctacatgtg
     3961 gtagatgtaa tatagactta attagtgttg taaatgaggt tgaagaaaag attggtagta
     4021 tggaaaaaaa tatcactgtt gcaattatgg gctgtgctgt aaatggacct ggtgaagcca
     4081 gagaagcaga tataggtatt gctgggggta aaggtgaagg tcttttattt aaaaagggtg
     4141 aaatagttag aatgattgat ggtaacaaat tagttgatga gttgctagaa gagattgaaa
     4201 aattatagta tacaattcat atatatatta aatgactaga tttttaaata cttttgtaat
     4261 aagtttttat ttacagtatt taattatcta gtcatttttt attttataaa cttcattaaa
     4321 tgtaag
//
LOCUS       DAYASV010000210         4308 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYASV010000210 DAYASV010000000
VERSION     DAYASV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.215"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            375..2474
                     /gene="nrdE"
                     /locus_tag="ACGI68_003611"
     CDS             375..2474
                     /gene="nrdE"
                     /locus_tag="ACGI68_003611"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFH0140013.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDKWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2487..3458
                     /gene="nrdF"
                     /locus_tag="ACGI68_003612"
     CDS             2487..3458
                     /gene="nrdF"
                     /locus_tag="ACGI68_003612"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFH0140014.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3534..4160
                     /locus_tag="ACGI68_003613"
     CDS             3534..4160
                     /locus_tag="ACGI68_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0140015.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1722 a          462 c          791 g         1333 t
ORIGIN      
        1 atcatcttga aagtactttt ttcaatatat cagaatatat ttttaagcta tttagagata
       61 atttaaattt ttaaacagga cataataagc ttgtattgtt ttatatttga gttgataaaa
      121 atttctttaa ataaaaataa tgtcaattat aaagtaataa aagtttagat ataaaaaaat
      181 ttgtgtatat actatatgta gtatataata gataaagcac tgactatatg tagtgctttt
      241 gtttttgaaa aatacttatt gcatttctat aattatagat taattttggg atttcaactg
      301 ataagtacca aaagaaataa taagcaaatt aagtgaaatt atggcacaag attatgttaa
      361 aaggacggta aataatgaat tggatagagc ttaacaatca agtgataatt aaaaatgaac
      421 aaggcaagta ccaattggaa aaggataagg aagccttaag tagttacata gaagaattta
      481 tacagccaaa gttgagaaaa tttaataacc tagaagaaag attgaagtat ttaatagatg
      541 aaggttatta ttcaaaagaa gtaataaatc agtatagcat agatgttatt aaaaatatat
      601 atgatatgat tgataaacat aagtttgaat ttcaatcata tatgagtgca aataagtttt
      661 atcaaaatta tgcattaaag agcaatgatg gaaaggaaat attagaaaca tataatgata
      721 aagtgttaat tgtagctttg acactaggaa atggcgataa agatttagct ttaaacttgg
      781 cagataaaat agtaaaacaa gaatttcaac cagctacacc tactttttta aatgctggaa
      841 gaaaaagggc tggagaaatg gtatcatgtt ttttactatc tgtagaagat agtacagaag
      901 gtatatcgta tgctatatct tcatcaaatc acttatcaaa gattggtgga ggagttgcac
      961 taaatttatc taaacttaga gcatctggcg aatctatcaa agatatagaa ggtgcagctg
     1021 gaggtgttgt tggagtagct aaaatgttag aacaatcatt tagttacttt aatcaaatgg
     1081 gagctagaca aggttctggt gcggtgtacc ttactgtatt ccatcctgat tttgaattgt
     1141 tgatggatac taaaaagata aatgcagatg aaaaaataag gcttgctaca ttatcattag
     1201 gagctataat accagataaa tttatggagt tggcagaaaa aaatgaagta gcatatgcat
     1261 tttatcctca tactgtgtat aagaaatatg gagtttctct tgatgagata gaaatggata
     1321 agtggtatga taaactggtt aatgatagtg atataagaaa aaaagaaata aatcctaggc
     1381 agatgttgac aaaaatagca cagatgcaac aagaaagtgg atatccatat gtagtgtaca
     1441 tagatacagc taatagagaa catacattaa aagatgttgg aattattaaa atgtctaact
     1501 tatgttgtga gatatttcaa tatcaaacac cttcagaaat agaggggtat ggaggaaaaa
     1561 atgaatgggg acaagatata agttgtaacc taggttcttt aaacatagcc aatgtaatgg
     1621 ataataaaac tatagaaagt acagttgaaa cagcaataag agcactgtca tttgtagcag
     1681 atagcacaga tataaaacca gtgccaacgg taagcaatag taacagcaag tctcattcta
     1741 taggattagg agctatgaat ttacatggat acttagtaag agaaaatatt ttatatactt
     1801 cagatgatgc tatagatttt tctaatgtat tttttgcaat ggttagatac tattcaatta
     1861 aagcttctat gaaaatagct atagaaagaa atcaaacttt tgaaggattt gataaatcag
     1921 aatatgttaa gggaagaaat agcaaggttt tatcaaaata ctatgaacaa tcttatctac
     1981 ctaagtcaga aaaggtaaga gcattatttg aagggattta tataccaaca aaagaagact
     2041 ggacaaaatt attggatgag gtaaaagaaa aagggatata taatgcatat ttaatggcag
     2101 tagcaccaac tcaaagcatt agttatgttc aaaatgctac atcaagtata atgccaatta
     2161 ctgagccagt agaagtaaga acatatggag actctactac aatatatcca atgccatttt
     2221 taacaaatga taatatgttg tattatcaat cagcatacag gatggatatg agaaaggtca
     2281 tagatttagt ggcaacagtt caaaaccatg ttgaccaagg tatatcaaca acactgtttg
     2341 ttacagatga aaagacaact agagatatag ctagacatta catatatgcc tataaaaaag
     2401 gtttgaaaag tttatattac acaagaacaa agatgacaag agatactcat gagtgtctag
     2461 tgtgttcagt atagggagag aaatatatga catttaattt acataaaata cataatgcag
     2521 ttaactggaa tagagaagaa gatggattta cacaagcttt ttgggaacaa aatgtaaaac
     2581 aattttggct tcctgaagaa atatctgttt ctaaagacat aaaggtatgg aatgaactaa
     2641 gtaataaaga aaaggaatta tataaaaaag tattgggtgg tttaacatta ctggatacaa
     2701 aacaggggaa taatggaata ccatctatga tgagtttaac agaaaaccta caaagaaaag
     2761 ctgtcttgtc ttttatgggg actatggaag aaatacatgc aaaaagttat tcttctatat
     2821 ttatgactct actttctaat ttagaaatag atgaattatt tgaatggata gaaacagaac
     2881 caacattaca aagaaaggct gatttagttt tagctcaata cgaaaatact acaaatcaag
     2941 agggattata cctatcaatg gttacaagtg tatttttaga gagtttcttg ttttattcag
     3001 gattctttta tccattgtat ctatctggtc aaggaaagat ggtagctagt ggagaaataa
     3061 tatcgcttat tcttagagat gagtctttac atggtaagta tataggattg ctagcacaag
     3121 aaatatacga ttcatttgat aaaatggata aggagatgtt agaagagaag atgtattcta
     3181 tactttatag ccttatggag aatgagatag agtatacaaa tgttatatac agagaaagtg
     3241 ggcttgaaaa agaggttgtg aactttttaa aatacaatgc gaatagagct ttagaaaatc
     3301 tagggtttga gaagctatat actgtagatg ctataaatcc aattgtatta aatggtctta
     3361 gcactgaaac caaaacacat gatttttttt caactaaggg taatggatac caaaaaggag
     3421 tgtatgagga attagaggat gaagatttta taatataatt tataattatg acacactgtt
     3481 gtcactttta gcgtgataat atcaatgtat aaaatatata tggaggtatt tttatgaaat
     3541 atgaatggag aaaaagagat aaagagttat atttacctaa aaagaatcct gtaatagttg
     3601 aaataccaga acagaaattt atcatgttaa aagggagtgg agacccaaat tctaaagaat
     3661 ttactgaagc aataggagta ctttattctt tggcatatgc aataaaaacc atgcctaaaa
     3721 aaggaattgt accagaaggg tattttgact atacggtttt tcctttagaa ggtatatggg
     3781 ataagggaga aaaaagtaaa ggcttagatg tacttattaa agaagattta atttacacta
     3841 taatgatacg tcagccagat tttgttacac aagacctgtt ggataaagct actgatatag
     3901 taaagaagaa aaaacctcat aatcttcttg aaaaagttag atttgggtct attaatgaag
     3961 ggctatgtgt tcaaatgctt catataggtt catatgataa tgaaccagaa agctttgaca
     4021 ttatgaacga attttgcaaa aaaaataaac ttataagaaa atcttacaca catcgtgaaa
     4081 tttatataag tgatgctaga aaagtatcgc ctgaatcatt gaagacggtt ttacgttttg
     4141 aagtagaaag ggaagtataa atattttgaa taatttataa gagttataaa aagtgaagtc
     4201 tgttgagact tcattttttg atgcaatttt aaaattaaat ttgatgtttc ataatatttt
     4261 aaaaactttc tacttttatt agtgtgaata actgttgcta ttaaataa
//
LOCUS       DAYASV010000211         4254 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYASV010000211 DAYASV010000000
VERSION     DAYASV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.216"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            273..611
                     /locus_tag="ACGI68_003614"
     CDS             273..611
                     /locus_tag="ACGI68_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HFH0140016.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1017..2453)
                     /gene="miaB"
                     /locus_tag="ACGI68_003615"
     CDS             complement(1017..2453)
                     /gene="miaB"
                     /locus_tag="ACGI68_003615"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFH0140017.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
     gene            complement(2701..4137)
                     /locus_tag="ACGI68_003616"
     CDS             complement(2701..4137)
                     /locus_tag="ACGI68_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HFH0140018.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLVNDGSNKSI
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGMYKVGKDIS
                     AGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMT
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
BASE COUNT         1389 a          668 c          451 g         1746 t
ORIGIN      
        1 ttacataaga aatttataaa agtttctaaa tttaacaatt ctaaaatatc aataactata
       61 ttttgtagca cttttttatt ttattattgt ttgaatattt gatggttttc aatgcaattt
      121 caacataaat actatatatt agtgtatact gttgatgaat taaagtatga taatctattt
      181 aataaagtat gaagtaaatg attttaatgt gcctttatgg taaaattact aataagtaat
      241 aaaattaatt aaaatatatg gaggtcctta taatgtttgc tgtaggattt gttgttataa
      301 ttgcacttgc ctttttcatt ttaagtgtac tattatttaa tggaaaagcc agtatgctac
      361 tagcaggtta taactctatg agtaagaaag aaaaagcacg atacgataaa aaaaaattat
      421 gtagaagttc tagcattgtt acttttattg tctctataat gctatttatt atggcgtttt
      481 tgggatatag aatagagagt ggtcaaatgg acgaaaaaaa aatggttccc ttttctataa
      541 tatttataat agtcatactt tccagtgtat tttttaatat cctctactct aacaaaaaat
      601 gtaaaaaata aatagttgta agttgagcag ttaattataa aagattgact gctttttcat
      661 gtatattgac tactcttatt aaatatttta acatttaaaa atctatgtaa aaataatgaa
      721 tatagtacaa acttctaata atgattttac ctttgtggta cagtccacta tatataattt
      781 aatatgataa cagggaatta ttaatgacaa ctaagtaaac ttttataaaa tataataaaa
      841 caaaaataga tgtacaatga aattatatct tatatcaaat ttatttttat taatagcaaa
      901 aacttacatt agcataacat atgacaaatt cgcttaaaat aaaaattttt aatataattt
      961 gttctatagt acatctattt tgattagttt acataatttt attattttgt acaatattaa
     1021 ttatttacta atatcccatt taatgaaaaa gttctaggtt cagtaatttt gaccataact
     1081 aatttaccaa ttaaatcatc atttccacct tcgaaattta cgagtttatt ttgtctagtt
     1141 ctaccagcaa acttattttc attattttta cttcttcctt caactagaac ttcaactatt
     1201 ctatctttat atccatcatt tatttctgca gaaatcctat ttacagcttc taacacccta
     1261 ttaaatctat catgctttat atcttctggt atttgatttt ccattttagc agcaggagtt
     1321 ccctgtcttt tagaataaat aaatgtaaaa gctgaatcat atctaacttt ttcaaccaca
     1381 tctaaagtgt ccaataaatc ttcttctgtt tctcctggga aaccaatcat taagtctgta
     1441 gaaaaagcaa tacctggaac ttccttttta gctttttcta ttatttctaa ataatattcc
     1501 tttgtataat gtctattcat ttttttcaaa aggctactgc ttccgcattg tattggtaga
     1561 tgtaaaaact cacacacctt atcacaatct ctaattgcgt atattacttc atcagatata
     1621 tcttttggat gagatgtcat aaatcgaacc ctttcaattc cttctatatc attcactttt
     1681 cttaaaagtt ctgagaatga aactggattt tcaagtgtct taccataaga atctacattt
     1741 tgtccaagta aagtgatttc tttcgttcca tttgcaacaa gctcttttat ctcgttaatt
     1801 atatcttcag gagttctact tctttctctt cctcttgtgt atgggactat acaatatgtg
     1861 cagaaattat tgcatccata cattatattt acaaatgctt ttaattcaaa ttttctatta
     1921 cttctaagac cttctattac ttctccatcc acatcccata catccactaa cattttgtca
     1981 gaatttattg attctgtaag taattgcgga aatttatata gattatgtgt tccaaatata
     2041 aggtctacat gtttatattt ttttcttaat tcctctacaa catgtggttg ttgcatcata
     2101 catccacata cagctatttt catatctgga ttttttcctt ttagatgttt taattgtcct
     2161 aagttaccat atacttttag ctctgcattt tctctaactg cacaggtgtt atatattatt
     2221 aaatcacttt cttcaaccat cattgacatt tggtatccca tctcttctaa cattgaacat
     2281 aatttttctg aatcatgttc attcatttga cacccaaagg tttgagtaaa cacgagtttt
     2341 ctctttccag ttctgttgaa gtattcttca ttttgtctgt gtatttgttc tatatattta
     2401 tcttgttctt gtattttctc tataggtatt gttacttgtt ttcttttgct cacgtctatt
     2461 cctcctagtt aatttcttat attacacata accttaatta tatcaaataa gaaaaatctt
     2521 ttaaagatat tattattttt ctagttgtgt tttaatagta aatctaatga tttatatata
     2581 ctatatttct aaaatataaa atcatataat tattataata ataaaaagat gttttaaatt
     2641 aaaacttaaa acacctttcc ttaaataaaa tatatgattt actattaaat acaattacat
     2701 ctattcattt tcaattaaat aacaatcatt taatattaaa tattgacctt cctcaaccgt
     2761 tatatatctt cttcctgaaa aagtatcatt agtaactatt ccctctgcat tacctaagct
     2821 atcatttcta acttcataat atgcatcaga agaatttgac attataatat attctccagc
     2881 aggaatttct aatccaattt tatacatacc atttccaaac ttaccattct tggccttctg
     2941 ggctttagca tactttgcta atgtcattgt tgaatcctca attttaatat attgaccatt
     3001 ctttaaggtg atatatctag tcccactaaa aatatcattt gaaagtatac tatcagcatt
     3061 acctgtacta tcacttgtaa cttcataata agaagcataa cttcctgagt ttgaagttat
     3121 taagtattca cctgctgaga tatcttttcc aaccttatac attccatctt ttatagtctt
     3181 tgtagtaggt ttaacatttt ttacaacccc agtattaata tctccaattc catttgttat
     3241 atttaaacac tgttgtacta tatttgaatc tatatatcct atagaagtta tcttcttagc
     3301 attttttaaa atgcttttat tgctaccatc attaactaaa accattggac catgttttga
     3361 taaagaagat cctacaagtg catttgttaa ttcataagct ccagctatat aaaactctct
     3421 agcatcctta taaaacttat ttattattgc tttattagtt tcaaaacgag tactcccccc
     3481 tagtctagta gatttagtat tgtttactaa agtggtactc attgaagctg ttcctcctat
     3541 tacataagac tttaaaccac tagttgaaaa aggtatactt tgtccattag taagaattat
     3601 tggtgcttta tctcttgctg ctacagatgc aatacttata gcatctgctt ctcctttata
     3661 tgcattggtt agcataacag tattgacttt ttttattgaa tttatttctt ttgcaacatt
     3721 ataacttgtt tttattctgt catttccctt aattcttaca actttcattt tcttagattt
     3781 taatgaattt tcgacagatg tactaataga atatgttcct ccaattatat aaacttttga
     3841 tacatttttt aatcttgcag atgtctcagt tggtatagta tttttttcag ttaatagtat
     3901 aggggcattt aaagctcctg ctagtccact tgcacttaat ccatctgcaa tagagttact
     3961 agtattaatt aatatagcag ttttatatgc atttttatct gctattttac ctgcgatttc
     4021 atacttattt tttccttgta ttttttctac agatgatatt gcatctacta attgaatatt
     4081 tgcaaataca aaagaaatac ttaaagtcaa tgttaatatt cttttataga atttcataat
     4141 aaaattgtcc ccctcataat aaatttttaa tatataaata catattctta aattagttat
     4201 gtacttaaca aacttatgta aaattgcaaa ttatattaag catattacaa ataa
//
LOCUS       DAYASV010000212         4165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYASV010000212 DAYASV010000000
VERSION     DAYASV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.217"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            120..1739
                     /locus_tag="ACGI68_003617"
     CDS             120..1739
                     /locus_tag="ACGI68_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFH0140019.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2113..2307
                     /locus_tag="ACGI68_003618"
     CDS             2113..2307
                     /locus_tag="ACGI68_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0140020.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2866..3189)
                     /locus_tag="ACGI68_003619"
     CDS             complement(2866..3189)
                     /locus_tag="ACGI68_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HFH0140021.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3234..3488)
                     /locus_tag="ACGI68_003620"
     CDS             complement(3234..3488)
                     /locus_tag="ACGI68_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140022.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3752..4033)
                     /locus_tag="ACGI68_003621"
     CDS             complement(3752..4033)
                     /locus_tag="ACGI68_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140023.1"
                     /translation="MASKQIPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         1502 a          547 c          487 g         1629 t
ORIGIN      
        1 aacattaata tttttattga caaaatttgt tattctatat tatggaaaca ataaatttta
       61 gttaataaag tttataattc agataaaaaa attattaatt tcttaaggga ggaaatatcg
      121 tgaatatagt aattagcctt atcggtggac ttggactttt tctttacgga atgagtttaa
      181 tgggagaagg tttacaaaaa tcagcaggag acaaattaaa aaaaattatt gaactcttaa
      241 caagtaatgt tgttatgggt gtactggtag gtacagtcgt tacaggtatt atacaaagtt
      301 caagtgctac tactgttatg gttgtaggat ttgtaaatgc aggtataatg aatctaagtc
      361 aagctattgg tgtaataatg ggggcaaata taggtactac agttactgct caattagttt
      421 catttaatct tgaaggaatt gcaccaattg ctttaggtat aggtatttta ttctatttat
      481 ttacatcaaa tcaaaaaaca aagcatttat ctgagatact tataggtttc ggtatattat
      541 ttacaggaat ggagtttatg aaagatgcag tagctccact tgctgaatat aaagcattca
      601 ctgatgcact tttatatttt agtaaaaatc ctgtattagg tatattggca ggatttgcca
      661 taactggtat tgttcaaagt tcaagtgcat ctatgggtat gttaatagca ctagcttctc
      721 aaggaatact tcctctatca tctgctttac ctattcttta tggagataat ataggtactt
      781 gtgttacttc acttttatca agtgttggtg caagtagaaa tgctcgacgt gcagctgtaa
      841 tgcatttaag ttttaatgta attggtacaa taatatttat gcttgtttta aataaaccta
      901 tatcagccat agttactcat tttgacccaa ctgatacagc tagacaaata gcaaatgcac
      961 atactttatt taatcttaca aatgttataa tattattacc attttctaaa tacatagtta
     1021 aattagcaaa tagacttata cctattaaag aaactgaatc agaaatagta aataatacta
     1081 agtacttaga tgaaaggatg tttagcactc catctatagc acttggtaat actgtacaag
     1141 aagttgtaag aatgggtcat aaagctacaa actctttgga acattcaatt gcaggatttt
     1201 taaataagag caatgaagat attaataaag cttttgagtc agaaaaagtt gtcaataagt
     1261 tacagaaaga tatattgaac tatttgttaa aactttctaa agaacctctt agagatgatg
     1321 aacgctttag aacagatttg ctttttaata ctgtaaatga cattgaaaga gtctctgacc
     1381 atgcagaaaa tatagctgaa ttggccatga gtgttaagga aatgaacatt agtttttctg
     1441 atagtgcgat aagagaaata tacgaaatat acaataagac aattactaat tttaaggatg
     1501 ccttaatagt tttagatgtt aaagattttg agttagcaaa taaagtttta gaagttgaaa
     1561 atgaagtaaa ttatttagaa aaaactttta gaaattcaca tatgattaga ctaaataatg
     1621 gaagttgtac aattgatgct ggtgtacttt atcttgactt acttactaat ctagaaagaa
     1681 tttctgacca ctcaacaaat atagtaaaac aagtactaaa acttaaacaa aaaatatagt
     1741 gattattttt atatataatc ttaatcaatg agaagtaaga agctatagat aatgcttata
     1801 tcatttatgg cttcttattt tttatgtaat actcaaattt ttatagtatt ctcttattat
     1861 taaacagttt ctgcctagaa tattccatat atatatgact tctttcataa aacttgaact
     1921 gcccataaat aaagtcacaa gtatacataa ctatttttta atcaattata accaatattt
     1981 gttttattta tcttcatttt aattcttcat tcaataatgt aataatatta ttttttattt
     2041 ggatactatt atatcatcta aatatgttat tatatatttg gatattttca tattaatagg
     2101 agggattcta caatgataaa ttatatggta gaaattatat atagcgaaaa aataagcgaa
     2161 gtagaaaaca tgttgaaaat tgaaaatata caatatgaat ttacttctcg tgatggcttt
     2221 atattttttg gtttttatga tatgacagac gcatatagat ttgagacttt atgttcagat
     2281 gaaaaaattc gatttaaaag aatataatag ataaaaaagt acatatttct aaactatttg
     2341 tttttcaaat ttaggttgtg ttttaatgta gtgttgatat tataaacaat tttaataata
     2401 aaaaagtagg gacattatat taatatgctc ctacttttta ctcatactta ttaatattat
     2461 ttatcaaaat taccctacaa actggaattt atcattcttt cataatttta aactatcata
     2521 tttaaaatat ataaaattct acatgatttt tgtataaaca taatatcaac cattatccaa
     2581 tttaaaaata ttttttagta atttccattt cattttctct taacatatta attaaacttt
     2641 tatatgtaaa tggtgtattt acatattatt taatatccaa caattatata aagaaatgtg
     2701 ctctttgcct tataaagaat tataaagcca attcgtttta ttatattatt tttctttata
     2761 ccttaaaaac tttaccttca aattcttatt tatctatata ttataaaaat gttcttaacc
     2821 tcttttacga gattttcttg acatattatc agttagataa aataattaaa taatataatc
     2881 agcaagtatg ttttttactc ttttaagtaa atttccacct gcaccccaat caatttttaa
     2941 aattccaatt gcactgcctg ttgaagtagc tttaataact gtttttcctg tggtattttc
     3001 ataaactact attaatcctg catcactatt atttcttgca taatacttct caaaagttgt
     3061 aattagtata aatttatcat ctcctaaatt ataagaatca ctaaaaacta aatctgcatc
     3121 aagattttct tttatcattt tttgtgcctc taagggtgta atttttacag taaatatttc
     3181 tgttgccatt ttttatctcc tcataagaat ttattttaga caaaacataa tctctagtat
     3241 tttctgatat atttatcaaa caatatccct attcctccta acagagctaa tatagaacca
     3301 aaatttctat attgagaaat agattggtct agataaatat tatattggtc agtatccatg
     3361 attccaccat tttgttgaat aatatgctct gcactaaatt cacctaaacc tatacaacag
     3421 aataatacta ctactccaat tacaaaagca aatatacata ttgttaattt aaaatcctta
     3481 ttttccatag ttatccctct cataagctaa atttatttga ttatttttac tagattttat
     3541 attactctta ttatacaata caacatatat ttacatctat ttccaaaatt gtaaccttta
     3601 tagttttata tatttaaatt ttttaaacaa aaaatacaca tctataaatt ataaatgtgt
     3661 attctgtacc attactaata atatattata cttttttaca aatttctaat tatttataac
     3721 tattcaaaaa taaaattcat ataaaataat tctattgtac aattgaaact ttaataatgt
     3781 ctccattttt tctctccaag tgaactactt ctccagttaa atcatagtca aatgccttaa
     3841 ctaatatttc agtaactttt ttaccatcta ctacatcatc ttttccatta aataaatcca
     3901 aagcattatt ctcaatcatt ctctttgcaa aatttacagg taactttata tgtattttat
     3961 cgcctgtatg tgataagact cttattctta aaaacttttc taaattgttt cttggtattt
     4021 gttttgatgc catgctatta aatcactctc ctttattttt tatttaataa taaattttat
     4081 tcttaaaaat atctttatat gtatttatta tgaaagtaac actattattt tctaatcttg
     4141 ttttatattc cttcttcttt aatat
//
LOCUS       DAYASV010000213         4141 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.218, whole genome shotgun sequence.
ACCESSION   DAYASV010000213 DAYASV010000000
VERSION     DAYASV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.218"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(40..1059)
                     /locus_tag="ACGI68_003622"
     CDS             complement(40..1059)
                     /locus_tag="ACGI68_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0140024.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(1235..1774)
                     /locus_tag="ACGI68_003623"
     CDS             complement(1235..1774)
                     /locus_tag="ACGI68_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HFH0140025.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(1875..2555)
                     /locus_tag="ACGI68_003624"
     CDS             complement(1875..2555)
                     /locus_tag="ACGI68_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFH0140026.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(2584..3873)
                     /locus_tag="ACGI68_003625"
     CDS             complement(2584..3873)
                     /locus_tag="ACGI68_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFH0140027.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         1385 a          735 c          378 g         1643 t
ORIGIN      
        1 agaatatata ttataaattc ttatattttc taatataaac tatatactat cagctggata
       61 aacaatacta ctttgtcttc ttatctcatc cataatttcc attactatta ttgaattttc
      121 atgagaattt atatttgatt ctattttatt gttatttata agacttataa actctacaag
      181 ttcataatac attccttcta tttctctagg cttttcctct gctttttctt ttggaataga
      241 tatatcttct tctcttccat ctctgtattt aataatagct ttttcaaata gattaagctt
      301 ttcaattatt atacttccct cttctccttg tatttcagat ggtatataag aatcagctac
      361 tttagaatac tgaattatcg catccatatc atcatattga aatacagctg aaccttctcc
      421 atccactcca gtttgaagta tatgagaaac tgcctttaca gtttttggag caccaaataa
      481 attcaccata ggatgtatac aatacactcc tatatccatt aaagctccat ttgacaactc
      541 ttttttaaag gcattttcta taattccatt tttatattta tcatatcttg aagaatactg
      601 acaatagctt ccaaaaaatc ttcttatttt ccctatttta tataagtttt ctttaactgc
      661 tttaaagttt ggaacacatg taattctcat agcttccata agcaatacat tattatcttt
      721 ggctgttttt atcattgatt tgacttcttt taaattagat gctaacgact tttcacaaag
      781 cacatgtttt ttgtgtttta aacaagtaat ggtttgcttt gaatgtaatg catttggaga
      841 agctatgtat acagcatcta tatccttcga tttagccatt tgttctaaat catcaaaaaa
      901 aatacttgct ccatatagag ttccaaattc tctagctttt tctaaatttc tagaatatac
      961 agcaaccaat tcaaagtctt taaccctaga tgcagctctt agaaatatat ttgctatatt
     1021 gcttgtccca acaataccaa atcttatttt acccatcata aatcactccc cattttctac
     1081 actttatttt aattttaata aaaaacttta caacttatag atatattata aaataatttt
     1141 atcaaataaa aaagaaagct aattttaaat gatttcacca ttttaacata gctttctttt
     1201 aactacaatc taaattcaac ttatattttt aaagttatct ttctactata tctttatttc
     1261 ccctcaaaaa ttttatagta agaatttcat ccacaaacaa tgttactaaa agtatagtta
     1321 caacaattgg ccaataaggt attataagac tatataaact atcaactata tacacttgaa
     1381 aatatctaac tccaataaat gtcaatatag tccaatatac agaaaacttt agacatacta
     1441 ttcctctact attgtactta taatctgaat aatcccaata gaccttatta aaatatactt
     1501 tcataagtat tcctgtaata tattctacta ctgtcggcac tactagacat aataaaagca
     1561 atgcaaatat attattgggg tcaataaatc ttgagacctc tattataatc gacattgcaa
     1621 ttgcatacat tggtttaaat ggaccattta aaaatccatc ttcttgaaaa tgcccttgtt
     1681 tataataact atataagttt tcaattatcc aacctataaa accatacaag aaaaaattaa
     1741 ataaaaaata taatagagaa cctacattat ccatgaatat tcctcctagc tgtttatact
     1801 tattttttct catattcatg gatttattct aaatcaaaga aattatattt aatttattta
     1861 attaatactt tatattatag attgtgacat tctttagagt tcaaactttt taggtattct
     1921 ctaacttcca gtaaattttc aagtatctta tgtgattgtg atttaatttg ctcatctggc
     1981 tcttttaaat ctggtacatt tatacatctt ataccaccat tgtatgcagc ttcaactccc
     2041 ataggcgaat cctctatgac aatacaattt tggggattaa catttatctt tttagctgct
     2101 tttaagaata tctcagggtt tggtttagag tttacaacat catctccaca aactatagca
     2161 tcaaagtaat cttttaaatt tgccttagct aatctcttta ctgctctttc tctctttgtt
     2221 gatgttgcaa cagccatttt gtatccattt tcctttaaaa aagagattag ttcattgact
     2281 cctaatttga taggagctcc cttcctctcc ataaattcaa tcatattttt agttttttcg
     2341 tcatataaat ctattattgg tacagaacta tcgtatatat ctgtaagacc ttctattatt
     2401 cctttacgat ttcttcccat aactgatgta taaatttcct tagtcatagt atatccatac
     2461 ttttcaaacg tctccatcca aaattcaaga gaaattctct ctgagtcaaa caaaactcca
     2521 tccatgtcaa aaataattcc ttctactttt tgcatacaat tcctccaata ttttttgata
     2581 taattatatt acacttttta tgattaattt tacaaccctc aatttatcca attctaatat
     2641 tgtaaaattg taattttcaa aagttacact ttggtttact ttaatatttg aaccaagcat
     2701 ataggatatc cacccaccta tactgtcaaa tccatcatgc tctatatcaa gtccaaaata
     2761 cttattaata tagttgatag attcagttcc atcaactaag taagaacctg ctcctatttt
     2821 cttaatatga gtctcttctt tgtcaaattc atcttgaatt tctccaacta tctcttctag
     2881 tatatcttct attgttacca caccagatgt accaccatat tcatccacaa ctatagcaag
     2941 ttgaagtttt tcctttctta ttctctcaag agctttatct atagttaaat tttcagatat
     3001 atatataata tctctcaata tttcttctag ttcaatttta ttttcattta tcttttgatt
     3061 gtataaatct cttatatgta caaaacctaa tatatcatct ttattttttc tgcatactgg
     3121 atatcttgta acaccttcct cttttaaaat agctaatatc ttttcttcag aatcactttc
     3181 atatatacat accatatcag ttctaggaac cattatctct ctaattttct tttcttcaaa
     3241 ttcaaaaata ttatcaacta atctttgctc tgattcatct ataagtccac ttttataact
     3301 ttcttctact aatagtctta tttcgtcacc tgtatggggt tcatcaacct catcagcttg
     3361 tgaatatcca aatggtttaa gaaataaatc agtacttaag ttgaaaaaat atattattgg
     3421 atatgttaac ttataaaatc ctactaataa aaaagatgta ctcaacatta ttttttctgt
     3481 attataaagt gctaaagctt taggaaccaa ctctcctatt acaacctcca tcattgttat
     3541 aattaaaaaa gaaatggaaa ttgatatagt atatataata ctttcactaa gattaaatac
     3601 acttactatt ggtaaaatta actctttaac tgtagattct cccatccaac ctaatgcaag
     3661 tgaacaaagt gttataccaa gttgacatgc agaaagatag gaatttaagt tgtcttttat
     3721 tattttacat ctttttgcat tattattacc ttcagttatc atggtatcaa tcctagaact
     3781 acgaagtttt accatagcaa attcacaagc tacaaaaaaa gcatttatca aaacaagcat
     3841 tactataagt gttactttta ataactcccg cataaagtat tggttcttat aatgtaagaa
     3901 atctataaga actcaaagtt ccccccttat attaataaat ttaatttata atatatattc
     3961 tatcatattt attaatatat cctactttat tacaaataat ttttataaac taaataaccc
     4021 cttttaatat ttatgactgg ctatttgata aatattaaag gggttattta gttatcattt
     4081 tttatttcta aaaatctttt ctcaaatgat ttagttatct atttatccaa ttttttacat
     4141 a
//
LOCUS       DAYASV010000214         4077 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYASV010000214 DAYASV010000000
VERSION     DAYASV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.219"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(324..704)
                     /locus_tag="ACGI68_003626"
     CDS             complement(324..704)
                     /locus_tag="ACGI68_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0140028.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(905..2098)
                     /locus_tag="ACGI68_003627"
     CDS             complement(905..2098)
                     /locus_tag="ACGI68_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFH0140029.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGICLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGESK"
     gene            complement(2161..3417)
                     /locus_tag="ACGI68_003628"
     CDS             complement(2161..3417)
                     /locus_tag="ACGI68_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFH0140030.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNACSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASSVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     regulatory      complement(3682..3758)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1483 a          741 c          421 g         1432 t
ORIGIN      
        1 atttttttaa caaactatca gagtagaatt atttaatatt ttgttatttt taatcaaatg
       61 aacgaatatt atatatatca ttcataaaaa ctttatataa agaatagaaa caagttctag
      121 attactatag agatgacata tcaaaatatt tttaaaaagt aatttaagta ttagaagtaa
      181 ttgaagttag taaatataaa tgcgtttact tttaagtatt gcttaaggga ataaaaaata
      241 aaaataaact ctgcaaccat ctaaaaatag ttgcagagct tacaaaacca aaattatcta
      301 aattgtaata aaaataataa catttataca taagcttctt cccaatttac tacccagttt
      361 tctaacgact ttatcttttc ctctgttagc tcctcttcgt gaaatgatac taataaattc
      421 cttataaaat tattcttcaa aagattttgc acatttcttg atataaactc taaatatgcc
      481 ttttctttta ttgcaactgt ataatacata cacttgcctg tttcttgaac atataagaat
      541 cttttctttg atagttttga taaagttttt aaagctactt tttctggtaa attatgttct
      601 tctttcatat aatcaataat ctcatatgaa ttggttttta tatctaaatt ccaaataaat
      661 ttcataataa ctaactctaa ttttgataat ttttttaaac acatagttat cactccgctt
      721 ctttatctat ataattaaat attacaataa taatttcaca aaatcaattc taataatcat
      781 aacatacctt aaaatctgtt cttaacttct accttcttaa tctcccaaat gattttttta
      841 tattgtattc aatagtataa tccttttcca aaatataact taccagttaa aataatcttt
      901 atttttactt actctcgcct tttacagaag ttaataattt tttataagtt acatcaattc
      961 caaaagctcc aagaggtgca gttataagta tagctagaac tgctaacgtc aaaataagtt
     1021 gtccagatgc catccccatt gcaagtggta cacctcctat tgctgcctgt actgtagcct
     1081 ttggaatata gccaatcata cagaacaacc tttcctttat tgtcaaattt gtcttaataa
     1141 ggcagaagaa tactcctacc atacggaata aaagaactcc aaatattaaa attacagctc
     1201 caattcctgc tttaaatgca taacttatat ctactgttgc ccctaccaaa acaaacaaga
     1261 gaatttcagc gccaacccat agttttgaat actttatgga caatcttctt gcaactacac
     1321 gatattttat ctgaaggaaa atcccaatac tcataactgc taataatcca gaaaatggca
     1381 caataccctc taaagcagtc tctaaactta tcattaaaaa tgagatactt aaaagcaaca
     1441 cccctttagc agaatctctc atatgaacct tcttaaaaaa tactgctaaa cagattccaa
     1501 tcactgctcc aactattaca cctaatataa ttgatactgg aatttgtaaa aaactaatag
     1561 ctgatatact atttccttgt gccaatcctg tgaatactgt aaacatcaca attacgaaaa
     1621 tatcgtctat agaagttcct gctaaaagca tctgtggaat acttttttct gtaccataac
     1681 cttcttccat taatttaagc attttaggta caataattgc gggagaaact gctcctacta
     1741 ctgcacccat gactgctgct tccaatatag aaacgcctag taattttggt gctaaaacta
     1801 tcattccgat aatttcaaaa gtagctggaa taaagcacat aagtacagca ggtcttccca
     1861 ctttcttcaa atcgtttata tctaaagata atccagccct tgttaatata ataatcaatg
     1921 caatctttct aaggtcagaa gaaatatcta gtattgaatt atcaatcaaa tttaaaacat
     1981 atggacctaa tataattcct gtaagtaaca ttcccaataa gttaggtaac tttatcctct
     2041 taaaaatacc tcccgaggcc attcctaata aaaaaatcaa agctaaactt gtcaacatat
     2101 tcctacccta tcctttctct aatattcttt ctcttctaat catacattat gttgttttat
     2161 ttattgtttt gttgaaattt tttgtagtga ataaatagat ttatccatat taaatatata
     2221 tgaaatagcg catactacaa agaaataagg taaatagctg tatccaaaaa cttctgcccc
     2281 tataaataca ggtgcaaaaa aagtatttgt agcactacca aagacactag catagccaag
     2341 cgctgcaact aattcaattg gcaaattaaa aaatcctgct aacaatacac caagactagc
     2401 tccaatggaa aacaaaggtg taacctctcc tccttgaaaa cctgctgaca aagtcagtat
     2461 tgtaagtata aactttaaca gccaatcaaa tgaataaatt tctccaccat agaaactatt
     2521 ttgaattaaa tttgtcccta aacctgaata tcttccttta taaaataaaa ggaataatac
     2581 actaagacat actccaataa tagcaattct tatcatagga ttttttaatc tattacctat
     2641 ttttcttttt gacaatttca gacaccatgc aaatgctcca ccaaccattc caaaaataat
     2701 tcctaaaaca ataagcttcc aaaatattga caagtcaaat acaactttag aagaaagtgc
     2761 aaaagaaaat ttctctaacc ctaaagcttt tgatactgaa cttgctgtaa aagaagcagt
     2821 aaacgcagga aaaagcgatt gatactccag tgaaccagct accaaaacct ccatagcaaa
     2881 tagtattgct gctattggtg tctcaaataa tcctgcaaaa ccagctgcca taccagttac
     2941 tagaaaaata ctacaagcat tttttatagg caacctttta cctacccaat gtgaaaaagt
     3001 tgcaccaatc tgaacagcaa ctccttctcg tcctgcacta ccaccaaata aatgggttaa
     3061 ccatgtacct gatattataa atggaaccaa tcttaaagga ataatttctt cttctccatg
     3121 ccctacctca aatattagat tcattccttt actacttttt cctccaaatt taaaatagca
     3181 ataagcaata acaactccta ctactgctaa aaatggtatc agatacattg gatatgtttc
     3241 tcgaatatct gtaactttta gaagaaccgt tccaaatatg gtgtctataa gacctataat
     3301 tgcaccaact ggtataccta tgagtcctaa aaaaaatagt ccaccatatg ttttataaac
     3361 gcttttaatt cttattatca ttttaatcca tattaaagtt ttaaattttt ctatcatttt
     3421 aattcctttc tatttgcatc ataagatttt ttgaaagttt tattacatca actgtagtca
     3481 atgtaatgac tatcataatt gttttttaaa ttgataggat ataggtacac tcttaaccca
     3541 ttcttaataa aataattaac atataatcta ctattttttc tattaattaa atatttcttc
     3601 aaatttaaaa aataaaaatt tagtaagtac ttcacctcca ttttattgat ttaaacaaca
     3661 aaaaaagctg gtggcatcta tgatatcata gaagtcatca gctcattggc agtttaggat
     3721 attatcatcc aagggagaac ctcattccca taattattat ataatttttt atttttcata
     3781 tgtctatttt atgtaaattg tatttttaat gttataaata atataaccta tcaattaaaa
     3841 tagtaaagcc tctaatatta tgagtgttca atatttcata ttatttttat gatatataat
     3901 ttaaatgatt ttctttactt tgcattatga tacataactt aaatattttt cctcactcca
     3961 cattatgata tattatttaa atatttttct tcactctgct tatattatga tacatgattt
     4021 aaatattttc ttcactctgc ttatattatg atacatgatt taaatatttt cttcact
//
LOCUS       DAYASV010000215         4051 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.220, whole genome shotgun sequence.
ACCESSION   DAYASV010000215 DAYASV010000000
VERSION     DAYASV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.220"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            58..1800
                     /locus_tag="ACGI68_003629"
     CDS             58..1800
                     /locus_tag="ACGI68_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloendopeptidase"
                     /protein_id="HFH0140031.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(1970..2158)
                     /locus_tag="ACGI68_003630"
     CDS             complement(1970..2158)
                     /locus_tag="ACGI68_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140032.1"
                     /translation="MKIITIGSSLITVLLFLSTMICGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYI"
     gene            complement(2155..2736)
                     /locus_tag="ACGI68_003631"
     CDS             complement(2155..2736)
                     /locus_tag="ACGI68_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0140033.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2904..3722
                     /locus_tag="ACGI68_003632"
     CDS             2904..3722
                     /locus_tag="ACGI68_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFH0140034.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
BASE COUNT         1540 a          519 c          552 g         1440 t
ORIGIN      
        1 ttttatattt aatatagtaa aataattaat aactatatcc atgattggag gatttttatg
       61 gttaaatcca agaagaaaat atttgttttt ctaattttaa ttgcagtttt tattatattt
      121 gcatcataca taagaaatta tactaatgat acaaaaaaag attctgttag aggaactatt
      181 ccttttgaaa aaatggaata taagcgtcct aatatacagt caatttgcga aaacattaac
      241 aagtgcaacg acaaactttt aacagctaaa acagctaagg aacaattaaa tttatttaat
      301 gaagttgata aaatttatca agatttttac agtacactta ctatagcaaa aattagaaat
      361 aacatagatt catcagacga attttactct aaggaatata agtatcttat gagtaaatca
      421 gtagatgttg acatgtcata caaagacttt caagatagat ttgtaacttc taaattctct
      481 aaagaattag aaaaagaact tggaaaggat acatttaatt atctaaagaa cattggaaaa
      541 cttaattcta aagaagttga aggtttgctt aaaaaagagc aagacctagt tgtaaaatat
      601 gaagacttat tagctaaatc tacagtatca atagatggta tagaggtaga ctttgaagaa
      661 gccttgtcaa gaccaaatct tagccctgaa gaatatgtga aaatttattc agattactta
      721 aaaaaataca atcctatatt tggtaacata ttcttagaac ttatacagac aagaactgaa
      781 atagcaagta agcaaggatt taagaattat atagattatg cctatatgaa cctaaacaaa
      841 gactattctc aagaagaagc taaaaaattt agacaagatg taaaagatta catagttcca
      901 ttatatagag agatttcatc taaaccatct gattcttcta tttatattaa agtatataaa
      961 aatcgctcat ttaggaagtt tgataaagta cttgaagata tatctccaaa actaaaagag
     1021 agcttcgact atatgaaaaa atatgacttg tacgattatt cttctggaaa aaacaaatca
     1081 cctggaggat atactactta tataaataaa tataaagcac ctttcttgtt taatacatgg
     1141 gataattctt ttttaggtgt tactagtttt gctcatgagt ttggtcattt ttataattac
     1201 tacaacagta ttaacttaaa caataagatg caaccaagta tagatgtatg tgaagttcat
     1261 tcaacatctt tagaaattct attttataaa tattttgatg atttttttgg taaacaatct
     1321 gaagctataa aaaaagaaca cttatcgatt gttttaaata ctattattga tgcttgtctt
     1381 tatgatgaat ttcaagaaat tatatacaag aatccatata tgagtctaaa tgatatcaat
     1441 aaattgtttt ttgatttaga ggaagaatat ggagtttcca ataatatact tagagaaaaa
     1501 aatgctcctt tttggatact tgtatctcat aacttccaag ttccttttta ttacttgagt
     1561 tatggtcttg catctgatgt atcacttcag atatggcaat tatcacaaga agattacaga
     1621 aaagctgttg atgtatatat ggatttttta aatcaaaata ctgatgctgg atttaaagat
     1681 gtggtagaaa aggtaaattt acaattacct ttccaagatg gaaatttaga ggaaataagc
     1741 tctactttat atgattattt tggtatagat aatccgcttg aactaaaaaa tgcttcttaa
     1801 tttctaatac tttatactca ctcttaagat ttagtattag attgaaataa gtgatatttt
     1861 ttcacacaca attaatgttt aataaaatta tatggtaaag tatcagaaat ttttaagact
     1921 cttgtcttta taatttctga tactttattt attaataata ttattatttt tatatatata
     1981 taattaaaag gattgtggaa atcaataaaa atatccctgt aaaaatagca ctattcatat
     2041 ggaacttaat agaacttgca tctgttacct tattattctt aatccagaac ccacaaatca
     2101 tagttgaaag aaaaagtaaa acagtgataa gacttgaacc aatagttata attttcatct
     2161 tacatcaaca tcctctccaa taatattttc tattaaaata tcaatgaatt tatctcgctc
     2221 atatttattc tttatattta taccactgta taactgaaat gattctgtag ataaagcaga
     2281 aatctgtatt ggtaaaataa tttgcagggc aataattctt agactcattt catctttttt
     2341 atttccaaaa attttacgaa gcatagggac aatatcaagt tctgcttgca tatctccatt
     2401 ggttatacat tgattcaata caaaaggaag cactttttca taatgtaatc cagtatcaca
     2461 caattttttt aataaatttt ttaaatcttc aataggtctt agagtatata cactatcgtc
     2521 atatagctct gctattatat ttgacatcac atctcctatg gcagtactca acaaattatc
     2581 ctttgtttta aaatgataat ttattaaacc aactccaaca tttgcacgct cagcaatttt
     2641 tctaactgta atctcctcaa tatcatctac ttcatttaaa agattcaatg taacttcaat
     2701 tatacgttct ctagcatctt tatcattact tttcataata gacctccaaa acaattaaca
     2761 ttttcagttc tgaacatgtt cagtataata tttttttcat catatgtcaa tataatttaa
     2821 ttataatttt agttgactct aacattatga tatggtttat attaatatta agctaaaagt
     2881 acctttataa aggagacaaa aacatgttta agataggcga tttttcaaag ctaagtaaaa
     2941 ttagtattcg tatgttacgt cattatgatg aaataggact tttaactcct tctcatacga
     3001 ataaaactaa tggatataga tactatagtg cagaccaact ttctactaca aacagaatcc
     3061 atgcactaaa agatatgggc tttggcttgt attctataaa agaaattttg acagaatata
     3121 acgataaaga aagtctcata aaatatctaa atatccacca ttctcaagta aaagaacaat
     3181 tagaagatac acagaaaaaa ctattaaaaa tagaaaccac tattaaaaga attggaggaa
     3241 atgatattat gaaaaattat gatgtaacta taaagaattt tgcaccaaaa tatatgatga
     3301 cacttagaag ggttatacca acttatcaag acgaaggaat gttatggcat caagcatttt
     3361 tagagacaaa agaccagaat gttcaaattg aacccccaaa gtattctaaa gcagtttttt
     3421 atgatactgg atataaagaa gattatgtag atgttgaagt acaagttgct gtatctggaa
     3481 agtataaaga tactgaacat gttaaattta agactgttcc aagtgtgact gctgcaactg
     3541 caattgttaa tggaaatttc aatcaagttg cagacgcttg tgaggctatt ggtaattgga
     3601 tatcagataa taactatgat gtggatggtc ctatgtttaa tatttatcat gtaagtcctg
     3661 gcaacgatag taatcctgat aattgggtaa cagaggtatg cttcccagtt aaaaagaagt
     3721 aatttttata ttaattggat aatattttaa tttgttagac aaattacttt tagtgttatg
     3781 tataaatacc tacaatttaa ttaggatatt atcttaattt gttaaacaaa ttacttttag
     3841 tgttatgtat aaatatctac aatttaatta ggatattatc ttaatttgtc agataaatca
     3901 cttgtgatgt tatgtttgaa tgactagaat ttaatcagga tattatctta atttgtcaga
     3961 taaatcactt gtgatgttat gtttaaatga ctagaatttg attgggatat tatcttaatt
     4021 tgtcagataa atcaattgtg atgttgtgtt t
//
LOCUS       DAYASV010000216         3955 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYASV010000216 DAYASV010000000
VERSION     DAYASV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.221"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            166..570
                     /locus_tag="ACGI68_003633"
     CDS             166..570
                     /locus_tag="ACGI68_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFH0140035.1"
                     /translation="MDKTLKKDLNQEYTESKSLIMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            587..1183
                     /locus_tag="ACGI68_003634"
     CDS             587..1183
                     /locus_tag="ACGI68_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140036.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNLDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            1220..3814
                     /gene="clpB"
                     /locus_tag="ACGI68_003635"
     CDS             1220..3814
                     /gene="clpB"
                     /locus_tag="ACGI68_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFH0140037.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNNFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
BASE COUNT         1667 a          381 c          710 g         1197 t
ORIGIN      
        1 aattggaaat actaaattaa aaaaataata aagtttagct tatagctaaa taaaagttta
       61 cttataaggt gaactttttg cattttcagt tttgacatac atattttttt gtaatatatt
      121 tatattatca attagagata aggagagtga gaagagatat ttaatttgga taagacatta
      181 aaaaaggatt taaatcagga atatacagag agcaaaagtt taattatgaa taaaagtaag
      241 tatatgaatg tttgttttaa gaattatatt aaagtagtag aaggaatgta tactaagagg
      301 gatttaattc cattaaaatt aataaactct atggataaaa ataataagat tagaaaaaca
      361 ttatatgaat tatcttgtga atttgaatat ccaaaaactt ctttatcttc attgtttacg
      421 gagctgaaaa aaatggattt tttacaaaga gtgaaaaatg gtgagtatat gataaatcca
      481 catatagcat ataaaggtag tagagctgat aaagaaagtt tattagcaga atataatcag
      541 ataaaaagac ctaataaggt tagtaaataa ggaagaaggt ttttatatga gtttaaaact
      601 tgatagaaat gttctacagt ggtttgacta tgtatttgag aatgaaaaaa ctagtttgag
      661 acactataat tttaattgta cactaaagga gattagttct accagtttga acaaagtagc
      721 ttttatttta gaaaaaaata actctaagta ctggaagctt tattttgaaa taccagctga
      781 agttacttta aaacttaagc aaaacattca tcctttattt agggaatata tatacgaaca
      841 aatatcactg tataacaata atcaaattta caattttgta aattcaaaca tattaaaagt
      901 gtttaataat attgctattt atcaatataa tattttagaa aatctataca ctattgattt
      961 taaaaagagt tttatagata aatgtcagta tttattaata ggtgaaaaaa gacttataga
     1021 tgaagacttg tatttaatag caaaatcaaa agaagtattc gattttttta atttagatgg
     1081 aacatttaat ttaacattga gtttcgatat acaaaaaaac gagaatttac ttgatagtct
     1141 tttagagctt agaaaatcaa taattataaa tgagagaatt taatttattt ggatataata
     1201 ttaaaatgga gggatttata tggacgtaga aaaaatgact ttaagagttc aaaaaagttt
     1261 aaatgaggct tataacgaag cagtaaaaaa tcataaccaa caagtagatg taattcatct
     1321 tttctcagca cttattaatc aagaggatgg attgatacca aatataatcg aaaaaatgaa
     1381 tatatcaatt gattctttga gaaatactgt aaactttgaa attgataagc ttccaaaggt
     1441 atatggagaa ggtgctgact cacaaggagt atcagctact agaaaaataa atgaggtgct
     1501 tataaaggca gagagtattt ctaaagaatt taaagattca tatataagtg tagagcatgt
     1561 tatgcttgca atgatggaaa cagaatcaaa atcagctgtt ggtaaaatat taaaacaata
     1621 taatataaat aagaataact ttttaaatat tttatcacaa gtgagaggta gtcaaagagt
     1681 agaaacacaa gacccagaag gcacatatga tgcacttgct agatatggaa caaatttagt
     1741 agatttagcc aagaaaaata aattagaccc tgtaattggg agagatgaag aaataagaag
     1801 gattataaga attttatcaa gaagaacaaa aaataatcct gtattgattg gtgagccagg
     1861 tgtaggtaaa actgcaattg tagaagggtt agcagagaga atagtaagag gagatgtccc
     1921 agaaggttta aaagataaga ttgtattctc acttgatatg ggagctttaa ttgctggtgc
     1981 aaaatataga ggtgaatttg aagaaagatt aaaggcagtt ttaaaagaag ttcaaagttc
     2041 tgatggaaaa attatactgt ttatagatga gattcatact attgttggag ctggtaaaac
     2101 agaaggttca atggatgcag gtaatcttat taaacctatg cttgcaagag gtgaattgaa
     2161 ctgtattggt gcaactactt ttgatgaata tagaaagtat atagaaaaag ataaggctct
     2221 tgaaagacgt tttcagcctg taattgcaga agaacctact gttgaagata caatatctat
     2281 acttagaggt ttaaaagaaa gatttgagat acatcatgga gtaagaatac atgataatgc
     2341 tattgtagca gcagcaaaac tttctcatag atatatacaa gatagatttt taccagataa
     2401 agcaattgat ttaattgatg aagctggagc tatgattcgt agtgaaatag attcattgcc
     2461 aacagaatta gatgttgtta gaagaaagtt atttacatta gaaactgaaa gagaagcatt
     2521 acttaaagaa aatgatgata aaagtaaatc aagattggat gatattcaaa aagaaatagc
     2581 agagttaaaa tctaaaaatg atgaaatgac agcaaaatat gaaaaagaaa agagtcaaat
     2641 attagatatt aaaaacttaa aggctcaatt agacgaagct aaaggtaaag ctgaaaagta
     2701 tgaaagagaa tatgatttca acaaagcagc agaggttaaa tatggtgaaa tacctaagtt
     2761 agaagaacaa ataaagcagt acgaagaaaa tatgaatgat ggttctgaaa attctctttt
     2821 aaaagaagaa gttactgaag aagaaatatc tagcattgta tcaaagtgga caggtatacc
     2881 agtaactaaa ctagttgaag gagaaagaga aaaattactt aaactagaag atgaacttca
     2941 taaaagagtt attggacaag atgaagcagt tacagcagtt tcaaatgcag taatacgtgc
     3001 aagagcagga ttaaaagatg aaagaaaacc aattggttca tttattttct taggccctac
     3061 aggtgttggt aaaactgaac ttgcaaaaac tcttgctaga aatttattcg atagtgaaga
     3121 taatataata agaatagata tgtctgaata catggaaaaa catgctgtat caagactagt
     3181 tggacctcct ccagggtacg tgggatatga agagggtggt caacttacag aagcagttag
     3241 acgtgctcct tattctgtaa tactatttga tgagatagaa aaagcacatg aggatgtatt
     3301 taacatgttc ttacagatat tagatgatgg tagacttact gataataaag gtaagacagt
     3361 agactttaaa aatactctta taataatgac ttctaatata ggaagtaatt atctattaga
     3421 agcaggagga aatataacag agactactaa caatttagta atgaatgaaa tgaagcatag
     3481 atttaaacct gagtttttaa acagagtaga tgatattata atgtttaagc ctttagacca
     3541 agaaaatatc aagaaaatta tagatatatt tatgaaagat ttaaaaaata gattaaaaga
     3601 aaaagatatt actattgaag ttacaaattc agcaaaagat gttatggtga gagaaggata
     3661 tgaccctgta tatggagcaa gacctcttaa gagatatata ggaaatacat tagaaactat
     3721 aattgcaaag aaacttattg ctggagatgt gtataatgga tgtactatag ttatagatgg
     3781 aaaagatgaa aatattgaag ttttagttaa ataataaaag aatcagttga gcataatata
     3841 ctaaatattt tgctcagctg atttttattt ataaactata ggaaaagaaa tattttatca
     3901 aattaataaa acatttttaa gtcaatatgt ccaaattaaa tttaactcaa aggaa
//
LOCUS       DAYASV010000217         3908 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYASV010000217 DAYASV010000000
VERSION     DAYASV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.222"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            457..1350
                     /locus_tag="ACGI68_003636"
     CDS             457..1350
                     /locus_tag="ACGI68_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HFH0140038.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1350..2744
                     /gene="gltA"
                     /locus_tag="ACGI68_003637"
     CDS             1350..2744
                     /gene="gltA"
                     /locus_tag="ACGI68_003637"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HFH0140039.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3110..>3908
                     /locus_tag="ACGI68_003638"
     CDS             3110..>3908
                     /locus_tag="ACGI68_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HFH0140040.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLV"
BASE COUNT         1541 a          393 c          753 g         1221 t
ORIGIN      
        1 taataaaata agagtatatt ttatacaata tgaaagctgg tattgcaata gattaaagcc
       61 ttatacacgt ttagttttta aagaaattag tgttattatg aaaacctaag ttaatttatt
      121 aaataaataa ttgaataaaa aaattttatt atgtatttat aaaatttttg ttacaaattt
      181 ggcataatat agtatacttt aagtgtgtat taggttaaat ctataacaaa ttagacgtat
      241 gatatagact tagtgtttat tggcatttac aagaaatttg ttaatatcaa ctatttgtag
      301 tttgggcagt tataattatt atagggggga tgtccaatac aaaacaatca accaagaact
      361 aatattatat tataaagagt tatagttttg tgtgtttatg tttttaatta aatttaaagt
      421 gggaaaaata tatttgttag aatggagaat agcgagatga gtaataaaat agttagtaag
      481 agacaattaa cagatagtat ttatttaatg gaaatagagg cacctagagt tgcaaaatct
      541 tctcaaccag gtcaatttat aataattaaa aatgatgaaa aaggagagag gattcctctt
      601 acaatagctg actatgatag agaaaaagga acagtaacta ttgtattcca aacagttggt
      661 gcttcaacta aaaagttagc aatgtttgaa gaaaacgatt ttgtaatgga ttttgtaggg
      721 ccattaggac aagcaagtga atttatacat gaagatatag aagaattaag aaataaaaag
      781 atattatttg tagctggtgg agttggttct gctccagttt atccacaagt taaatggttt
      841 aaagagcatg gattagatgt agatgtaata attggtgcaa gaactaaaga attaattata
      901 ttagaagatg atatgaaaaa agtagctaaa aatgtatatg tatcaacaga tgatggtaca
      961 tatggattta atggaagagt tacagaccta ttaaaagatt tagttgataa tcaaggtaaa
     1021 aaatacgacc aagcaatagt aattggacca atgataatga tgaaatttat gtgccaatta
     1081 actaaagaac taaatatacc tactatagta agtttaaata caataatgat agatggaaca
     1141 ggtatgtgtg gtggttgtag agttagtgtt ggaaatgaaa ctaagtttgc atgtgtagat
     1201 ggtcctgaat ttgatggtca tttagttgat tttgatcaag caatgagaag acagtctatg
     1261 tacaagactc aagagggaag agctatgcta aaacttgaag aaggcgatag tcatcatcat
     1321 agtaattgtg gatgtggagg taacaaataa tggatgctaa aaaagtaaaa gtaccagtta
     1381 gagaacaaga acctgctgtg agagcaacaa attttgatga agtatgctta gggtataata
     1441 aagaagaagc tatggcagaa gctaatagat gtttagcttg taaaaaacca aaatgtgtgg
     1501 gtggatgtcc agtaggaatc gacataccag ggtttataac aaaaattaaa gaagatgata
     1561 tagaaggtgc tgctaaagta atagctaaga gcagttcatt accagctgtt tgtggtagag
     1621 tatgcccaca agaaagtcaa tgtgaaggtg tatgtatact tggaataaaa tcagatgctg
     1681 tatcaatagg taaattagaa agatttgtag cagattggtc aaaagaaaat gacataaatt
     1741 tatctgatac agagcctaag aaaaatcaaa aagtagcagt aataggaagt ggtccagcgg
     1801 gtctagcttg tgctggtgat ttagctaaaa aaggctatga tgtaacaatc tttgaagcaa
     1861 tgcatgagcc aggtggagtt ttaacttacg gaatacctga gtttagactt ccaaaacaag
     1921 ctgttgttca acctgaaata gacaatataa gaaaattagg tgtaaaaata gaaactaatg
     1981 taattgttgg taaaacaatt acagtagatg aacttataga agatgaagga tttgaagcta
     2041 tattcatagg ttcaggagct ggtcttccaa tgtttatgaa tataccaggg gaaaatgcaa
     2101 atggagtatt ttctgcaaat gagtttttaa ctagagtaaa cttaatgaag gcttatagag
     2161 atgattatga tacacctatt agttctggta aaaaagttgc tgtagtaggt ggtggaaatg
     2221 ttgctatgga cgctgctaga actgcattaa gattaggttc agaatcatat atagtttata
     2281 gaagaagtga aaaagagctt ccagcaagag ctgaagaagt tcatcatgca aaagaagaag
     2341 gcattatatt taatacatta acaaatccta aggaaatttt agtagatgaa aatggatatg
     2401 ttaaaggtat ggtttgtata agaatggaat taggagagcc agatgattct ggaagaagaa
     2461 gacctataga aatagaaggt tctgaatttg tacttgatgt agacacagtt ataatgtcac
     2521 ttggaacaag cccaaatcca ctaatatctt ctacaacaaa gagtcttgat ataaataaga
     2581 agagatgctt aataacagat gaaaatggtc agacttctaa agagggtgta tttgcaggtg
     2641 gagatgctgt tactggagca gcaacagtaa tatctgcaat gggtgctggt aaaacagcag
     2701 cagcatcaat agatgaatat ttaaaagcaa aagttaatgc ttaatttgta aattaaagag
     2761 agggtgtctc aaaatgaact ttttagttca tgaggcactc tttttgtaca aaatcaaata
     2821 tgttttaatc atttcaacaa tgaaaatttc attcctgtag aaagtctaac ttttaggtct
     2881 tagtacaatc tctattttga tgtagccact tttttagatg gtttaataat ttatcagtta
     2941 ttattgtaat atttgatact aagtaacata actttgtcta actatgataa aatagttata
     3001 caataaaggt cttgtaagga gtgaataatg tgtggctaat aaaatcagat ttgtcaaatc
     3061 taacattaaa taatagaaaa ataaattgtg taattaatgg tattttaata tgaaaaaaag
     3121 agaaaaagta tttgatgggc tagtttttaa gcatatatta atcttattaa tactattagt
     3181 tttgattgat ttaacaagta ccatcatata tgcaagtaac tctatggaaa ttacttctag
     3241 aaatatgatt gaggcatcaa aaagagaact tgaaaactat ttagaagtaa acatatcttt
     3301 attaaaagct cttagtcaag atgatagatt ttctaatggt gaaacatcac taattgaaaa
     3361 gggaaaatta ttaagtccat atcaaaagga atataatctt tttatgattg gtataacaga
     3421 tacaaaagga aatgcttcaa gtacatatag ggaaaatgtt aactcaataa aagataagcc
     3481 tagttttgag aaagcaatta aaacaaagca agtagtagtt tcagatattg aagtcagtaa
     3541 tgtaacaggt gacaaagtat ttatcatata tgtaccaatt gtaaaaaata atgagatgat
     3601 tgggaccatt tttgcttctt tttattttca agatgtaaat aatttaataa gtagaagcaa
     3661 ttttgatgat tctattaaat ttctaatgat agataaagat tatactatta tttctcatcc
     3721 aaacaaaaaa tatgtaaatg ataaaagtaa gatgcttgat ttagaaggga atattatagg
     3781 aaccactaaa agtgaaatct taaaaaatat aaataaaaaa tgtcaaggtg attttttatc
     3841 ttgggataat tggagattat ataatgtaaa atatacgaac ataaagtgga ctaattggac
     3901 tttagtat
//
LOCUS       DAYASV010000218         3908 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYASV010000218 DAYASV010000000
VERSION     DAYASV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.223"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(129..347)
                     /locus_tag="ACGI68_003639"
     CDS             complement(129..347)
                     /locus_tag="ACGI68_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0140041.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(348..755)
                     /locus_tag="ACGI68_003640"
     CDS             complement(348..755)
                     /locus_tag="ACGI68_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140042.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(841..1329)
                     /locus_tag="ACGI68_003641"
     CDS             complement(841..1329)
                     /locus_tag="ACGI68_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HFH0140043.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(1534..2631)
                     /locus_tag="ACGI68_003642"
     CDS             complement(1534..2631)
                     /locus_tag="ACGI68_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFH0140044.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTKKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIAKGKREEKFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(3004..3174)
                     /locus_tag="ACGI68_003643"
     CDS             complement(3004..3174)
                     /locus_tag="ACGI68_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140045.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(3346..3789)
                     /locus_tag="ACGI68_003644"
     CDS             complement(3346..3789)
                     /locus_tag="ACGI68_003644"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0140046.1"
                     /translation="MIEIKTIEINKKSRIDLLMLADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         1336 a          641 c          352 g         1579 t
ORIGIN      
        1 tataaatcat tcattcttta atctatgagc ttggtttttc atctataagt catttattct
       61 ctaatctata agcttggttt tttacctata attaattcat tctctaatgc ataagtaata
      121 catgaaattt aaatattatt ttcttcaata aattcaaaca actcttctac agttgtatca
      181 aataccttag caatatccat tgccagtttt aaagaaggat tgtagcgacc attttctaaa
      241 tgtacaatag tttcacgtct tactcctact gactttgcaa gttcaccttg tgtcataagg
      301 ttcttttctc tataggattt aatttttgtc tttagtaata ctgacaccta attgtatatc
      361 ccctttctgt caaaatatat aaatagaatt aacttcaaca aggattgaat aaacaaaatg
      421 actaataaag acattcctat atttaaattt gacacattta tgtccaaagt tacaagaaat
      481 atagatacta tagctacaga aaaaaacaat acttttattg caatatcatt aactcttgat
      541 agtatacatt ttgatgcctc atcaattaaa tcatcatact ttttattata gacccactga
      601 gctcccatag ctaataccca aataattata ggaagatatt caatactact tcctttaaaa
      661 aaatacttat ttactaatgt agctccaata caataagaaa ttcccaatat ttctgaaata
      721 agtgttgatt taataccagt ttgttgttta gacatataaa atccccctta aaaagttatg
      781 tatttataac acctcttgtt ataaatatat aacttttttg aataaatttc aataaaaata
      841 ttattttgca acaaattctt ttattaattt tacattagat agaggcactt tagatgtcac
      901 tccttgcaat gtcaaatctt ctatattaac ttcatttaat tttgaacaca gttctaagtc
      961 aaatattctt tcccagctta ccttcttttc aatttgttca tcttcataaa gctctacagt
     1021 aatatccatt aaaggtaaat gccataaatc aaattctgat aataacacat tgtcttcact
     1081 tatttctatt tcaagtaaaa cacctttttt tcctttttct aaataaccac tacatcttaa
     1141 atcaggtctc ttcgtccaaa cccatatcat actatcatca aattcatata ttctatttgt
     1201 catttgacta tacatccatt tgtatggaat atcaaagtct tctaatataa aagaataatc
     1261 atttgataga tatttattgt ttttattgtt attccaaaat tctaaagtct gtatagtata
     1321 aaatttcata tataaatcaa cccctttgtt tttctataag aaggagatgg ttcaaacttt
     1381 tattttgaat tatctccttt atttatacac ctctttgtac ctattattta tatatctctt
     1441 tatatctacc atatttaaat aacaaagttt tatattaatt taaatttaaa agaggaagct
     1501 ttgtatttat ctagtgccat ctcaatcata gtattagttt tgattataga ctttttcaac
     1561 ctctgattta ttcaaatcat ctataatatc gcatatttca ctcaaattgt atcctttatt
     1621 ttttaatttt atgatgatat taaatttctc ttctcttttc ccttttgcaa tacctttttc
     1681 aataccttct tgaaaaattt cttctttaaa atttctactt ttattcataa tatacgcacc
     1741 tctctttaaa tttaataaat agtcctcatt actacctaat tcttttaatc tatcaagtgc
     1801 tcttttaata acataatccg ttttagatag tattctaata tctatatgag aaggattatc
     1861 aataagttta ccccatatta gaactctgtt tgctatacaa ctggatttac gaatttttgg
     1921 aagttctata aaatataatg cagttttact agaataaata tctggatgcc cgtccattgt
     1981 taatctcagg caatttacat aatcttctat gtgattatat tttacatgat ttagtatatg
     2041 tattccaaca gtttttccaa tttctttata ttctttacct tcttctaact ttctaccatg
     2101 tacacgacta agataatatt cagctctttc caaataatcc tcttcaagag ctctttgcat
     2161 ctcaatattt atatggttac gttcttcatc ttcagccaaa atatcaagtc taggtctttt
     2221 ctcaccaatg taattaattg ttgggtcggt ttcaacctta attattcttt ttatttgagt
     2281 gaactgtttg atattcctat tagcatatgg agcatctttg aggatatcat ttataaaatt
     2341 tgcaagtaaa tcgtaatcat tatttaaagt aaacaatgat ttaaatacct catcatactc
     2401 aggcttagca tacccatctt cagttacaac ttctccaaca ttttcactag tcaatatttc
     2461 ttttggaatt aaatccttga ttttatctaa tttttcatta gaaaaatcac ctctactaag
     2521 tatgtcattt aattttttgg tccattctct gtccttttct tcaattatag ttcgtttata
     2581 ctcttttgat gttttaataa tcttagattt ttcttctaat ttttctttca tatcaacacc
     2641 tcttttttat tattacccat ttcttcatac caaagtctga acttctaagt aaaatttgca
     2701 ttattgaaat aagaacctta taaacttata tatcattact aaagttaaga ctttaaagag
     2761 aagcacttag attacaatag attttagata gctttctttt taaaactatt gttgataaat
     2821 gaatgaatat aagggtatgt attaaaatag agatactcat ctttttcatt gtttaaataa
     2881 tgaaaatata ttttatttaa tacaaaaaag agtgccttat aaactaaaag ttcacttcga
     2941 gacactccct tacattttgt ttcttaaact ttacaatatt acatacttat ttattaattt
     3001 aatctatgca tacttaaccc aataataaag tacatatatc caactcatta agccatgaaa
     3061 aatagcccat ggaatagatt tccatgcagt ataagaaata atcattgcta aacaagaacc
     3121 aaaagaaata ccctttttaa cagttgattt tatagtatta ttatttgaat ccataattaa
     3181 tcctccaaaa ttcaaaataa acaagttatt atatatctta tttgaatata atacaatata
     3241 acagcttcta aataaattat actcttatta tacataattt ataaattata attccgataa
     3301 ttatatgaat taattaatta tattaaccta aaaatattat cctatctaaa agttatatga
     3361 tagatacaac atgtcaacac attgtaattc tccttcaaag attggttctg gataattatc
     3421 tacaaaaaag tttttaattt tatgtgaata aacaaatcca aacttctcat aaaaaggaac
     3481 tccgctttca gatgtaccaa caaccattga tgaatatttt ttactataaa catctaaaag
     3541 atgataaatc atctttgagg cataacccat attttgaaaa tgctcatatg tagcaatgtt
     3601 ttttaattca catatttctt tggaaatttc atttacaact gctatacaag taagttcttt
     3661 attatcatac aatgcgtaca tagttccttt attgagatat ttatcaatca tttcttcaca
     3721 tgggtcggct aacattaaaa ggtcaattcg acttttttta tttatttcta tagtttttat
     3781 ttctatcata agttctccac cctcacttta ttcaaacctt aatttaacaa acttcttaca
     3841 gtacttattt aattcatttt gatatatatt tttacttttt aaaatatagt gataattccc
     3901 tagaatat
//
LOCUS       DAYASV010000219         3873 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYASV010000219 DAYASV010000000
VERSION     DAYASV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.224"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            151..498
                     /locus_tag="ACGI68_003645"
     CDS             151..498
                     /locus_tag="ACGI68_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFH0140047.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(648..1127)
                     /locus_tag="ACGI68_003646"
     CDS             complement(648..1127)
                     /locus_tag="ACGI68_003646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0140048.1"
                     /translation="MAGAFAGGEGDFTTAFEPTATAMEKEGSGYIVTSIGKDSGEIPF
                     TAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKAMQPFFEDISLEDLTTV
                     VDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKDIVNTEFANNAIKNIK"
     gene            complement(1175..2680)
                     /locus_tag="ACGI68_003647"
     CDS             complement(1175..2680)
                     /locus_tag="ACGI68_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0140049.1"
                     /translation="MNVAIYLRKSRADEEAEHRGEFETLSRHKTTLLKLAREQNLNVI
                     EIKEELVSGESIEYRPKMLELLGEIKNNNYDAVLVMDIDRLGRGNMQDQGLILDTFKK
                     SKTKIITPRKTYDLNDEFDEEYSEFEAFMARKELKLISRRMQRGKIKSIEEGKFIAQA
                     PPYGYDIKYLNNKERVLVINEDKATVVKIIFDMYLKGDGACKISNYLNELGYKTTTGR
                     TWYEKGIRDIIKNKVYCGYIVWNKVERKRNSSRTRGIEEQIEVKGKHEPIISEDIWNK
                     AQNIRKKRINSPTPNNKITNPLAGIVKCGYCNHTLIAKSNTTSNGYEKYMVCTNCKNK
                     ATKLYILENKILEVLNKWLVNYRLSLKDEKDNSKPENTLEHTLINLNKELDTLEKQKE
                     RLHDFLEQGIYDSNTFGERLKILLSRIENAREAINKTNIDIEMQKERNTVKTQIVPQL
                     EKILSEYTEEKEPYLKNILLKSVINYAVYKKEPNQKSDDFSLILYPNIPRD"
     gene            complement(2879..3733)
                     /locus_tag="ACGI68_003648"
     CDS             complement(2879..3733)
                     /locus_tag="ACGI68_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0140050.1"
                     /translation="MEWIERLNKAINYIEENITKEIEYEQVAKIACCSTYHFQRMFAY
                     MADVPLSEYIRRRRMSLAAVELQNDDKKIIDVALKYGYSSPTAFNRAFKSIHGVAPSV
                     IKKREITTLKAFPPISFKISIKGAEEMNYRIEKKEAFRIVGVSQPLHNELEKNFEIVP
                     QMWQKVALDGTLQKLIPMMDSQLQGVLGISICNNSEEWKYFISVSSTKSIDNTLEEYT
                     VPAFTWAIFSGEGQCPQAIQELEKRIVTEWLPTSGYEYDNGPDIELYLNPDPQNAKFE
                     VWIPVVKK"
BASE COUNT         1237 a          612 c          467 g         1557 t
ORIGIN      
        1 taagaattgt atttttctaa ctataaaaat agtattatac ttatatttaa aaataaaaga
       61 attttattcc tattgtgtaa agtgagaata ctattttctt atattggtat aataatagta
      121 aatctaatat agaaaagagg tatttctagt atgcatgaaa ataaaaaaaa tctattagac
      181 ggaagcttag ctcaatataa tgatacagca gttccaaaaa taccagtatt tgctggtaac
      241 gatataactt cagaagttta ttattttaaa cctaatcaag ttcttaatgc tcatagacac
      301 cctaatggtg aacaaatatt tgtgttccta aaaggagaag gtaaaatgaa acttggtgag
      361 catgaatgtg atgtaaaaaa cggcgatact gtatttgtac caacaggaga gtggcatgaa
      421 attactaatg gaagcaatga agaaatggta gccgttcaaa ttacaaaaat taatgctgga
      481 gcagaataca gaggatagat atataagtac agttagtact ttaatatcta ttttattata
      541 taaactttaa tctcaaatat aaaaagagtc ttttaaaagg ctctttttat atttttgtta
      601 attttcatta taaaaacaag aaaatatcta gctaaaacta atatatttta tttaatattt
      661 tttatagcat tgtttgcaaa ctcagtattt acgatatctt tataaggtgc ttccttatct
      721 agttctttag cttctttcat aacagtcatc aatttcttca aaccttcctc ttttaataca
      781 ggattttgag accatgcatc tattgattta tatctatcta caactgttgt taaatcttct
      841 aaagatatat cttcaaagaa aggttgcata gccttagcta cttctttgct agatgcttgt
      901 tgaatccata attgaccttt ataaacagca tttgtaaatc tttgaattaa atctttattg
      961 tttttcatgt atgatctcgt agtagagtat gctgtaaaag gtatttctcc agaatctttt
     1021 cctatagatg taactatata tccactacct tctttttcca ttgctgttgc tgtaggttca
     1081 aatgctgttg taaagtcacc ctctccacct gcaaaagctc cagccattac accaaattgg
     1141 taatcagtct atttataaca tttaaaacat ataattaatc tcttgggata ttagggtaaa
     1201 gtattaaaga aaaatcatca gatttttgat taggttcttt tttatagact gcgtaattta
     1261 ttacactttt aagcaatata ttttttagat aaggttcttt ttcctctgta tattcactta
     1321 aaattttttc aagttgagga acaatttgtg ttttaactgt atttctttcc ttttgcattt
     1381 caatatctat atttgtttta ttaatagctt ctcttgcatt ttctatacga gataagagta
     1441 tttttaaacg ttccccaaat gtattactgt cataaattcc ttgttctaaa aaatcatgta
     1501 gtcgttcttt ttgtttttct aaagtatcca attctttatt taaatttatc aaggtatgtt
     1561 ctaaagtatt ttcaggttta gagttgtctt tttcatcttt taaagatagt ctataattta
     1621 ctaaccattt atttaagacc tctaatattt tattttctaa tatgtatagt ttagtagctt
     1681 tatttttaca attagtacaa accatatatt tctcatatcc atttgatgta gtattagatt
     1741 tggctattaa agtatgatta cagtatccac attttacaat tccagccaat ggatttgtta
     1801 ttttattgtt tggagtaggg gaattaatac gtttttttct tatattttgt gccttattcc
     1861 atatgtcttc acttattatt ggttcatgct ttccttttac ctcaatttgt tcctcaattc
     1921 ctcttgttct actagaattt cttttcctct cgactttatt ccatactata tatccacaat
     1981 acactttgtt ttttataata tctcttatac ccttttcata ccaagttcta cctgttgtgg
     2041 tcttataacc aagttcgttt aaataattgg atattttaca tgcaccatct ccttttaaat
     2101 acatatcaaa gattatttta acaacagttg ctttatcttc atttattaca agtactcttt
     2161 ctttattatt taaatattta atatcatacc cataaggagg agcttgagct ataaatttac
     2221 cctcttcaat acttttgatt tttcctcttt gcatacgtct agatattaat tttaattcct
     2281 tacgagccat aaaagcttca aattcagaat actcctcatc aaactcatca tttaaatcat
     2341 acgtttttct aggagtaata attttcgttt tagatttttt aaatgtatct agtattaacc
     2401 cttggtcttg catattacca cgacccaaac ggtcaatatc catcactaaa acagcatcat
     2461 agttattatt tttaatttca cctagtaatt ctagcatctt aggtctatat tctatacttt
     2521 ccccagaaac aagttcttct ttaatttcaa tgacatttaa attttgttct cttgctagtt
     2581 ttaaaagagt tgtcttatgt ctacttagag tttcaaattc acctctatgt tctgcttctt
     2641 catcagctct gcttttgcgt aaataaattg ccacattcat taaatcacac cccttgaata
     2701 tattatattc agtaacagat tgattatatt taaaacacaa aaataaacct tcaaaataca
     2761 agatatattt tttaggttta tttttataca gtaaatttta tagtatattt aaaagcttgt
     2821 aatctaagta aatatttata ctattaattt tatgctttct tcatttttta attttaaact
     2881 atttttttac tacaggtatc catacttcaa atttagcatt ttgagggtct ggatttaagt
     2941 acagttcaat atcaggacca ttatcatatt cataaccaga agtaggtaac cattctgtta
     3001 caatacgctt ttctagttct tgtattgctt gtggacattg tccttctcca gaaaatattg
     3061 cccaagtaaa agctggaaca gtgtattctt ctaatgtatt atctatagat tttgtacttg
     3121 aaacagatat aaaatacttc cattcttctg aattattaca aatacttata cctaatacac
     3181 cttgaagttg actatccatc atagggatta atttttgaag cgttccatct aaagcaacct
     3241 tttgccacat ttggggaaca atttcaaaat tcttttctag ttcattatga agtggctgag
     3301 atacaccaac tatacgaaat gcttctttct tttcaattct ataattcatt tcttcggctc
     3361 ctttaatact aattttaaaa ctgattggag gaaaggcttt taaagttgtt atttctcttt
     3421 tttttataac tgatggggca acaccatgaa tacttttaaa tgcacgattg aatgctgttg
     3481 gagaagagta tccatatttt aaagccacat caattatctt tttatcatca ttttgtaatt
     3541 ctacagctgc taaagacatc cgtctacgac gaatatattc agaaagtggt acatctgcca
     3601 tataagcaaa cattctttga aaatgatatg tagaacaaca agctattttt gccacttgtt
     3661 catactcaat ttcttttgtt atgttttctt caatatagtt aattgctttg tttaatcttt
     3721 caatccattc cataaaatca gctccttgta atttatataa tatctatttt aaaatagtta
     3781 atcctctctt tctatgcact aattaactag cttaataaag aatgaacatg agaaagtctg
     3841 taattaacta caaaaatgaa caaatagtat tgt
//
LOCUS       DAYASV010000220         3853 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYASV010000220 DAYASV010000000
VERSION     DAYASV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.225"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            111..1223
                     /locus_tag="ACGI68_003649"
     CDS             111..1223
                     /locus_tag="ACGI68_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0140051.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1431..1907)
                     /locus_tag="ACGI68_003650"
     CDS             complement(1431..1907)
                     /locus_tag="ACGI68_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140052.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2106..3731)
                     /locus_tag="ACGI68_003651"
     CDS             complement(2106..3731)
                     /locus_tag="ACGI68_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFH0140053.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALLNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1346 a          613 c          424 g         1470 t
ORIGIN      
        1 ttaaataaat atatacaatg tattaaaatg tatgcaactc tatttcaaaa ttattttgtg
       61 atatatatat gttataataa atgtataaaa ttttgattgg tgggttatat atgaatgcat
      121 ttattagaaa aagaaataaa aactatgttg tttacctgga atttgaagat gaaaagacag
      181 gtaaaagaaa acaaaaaaat atgggtactt ttgataaaaa aagggaagca agtaaaagac
      241 taaatgaggt aaaagaaagc ttatataatg atggtttttt agtaccaagc gaaattacag
      301 tgtcagaatt tctattagat tttctaaata aatatagtga aaatatatct ttagctacat
      361 ataataatta tgttagtatt tgtaagaatt acattaatcc atctattgga aaatataaaa
      421 tacaagactt acatccggtt cacatacaaa actacataga taaactatct tataaactaa
      481 atcctcaatc aattaagatt catataaata tattgaaact tgcaataaaa agagcttata
      541 gactcaaatt aataaaagaa aatgttatgg atagtataga ggctccaaga tataaaaaat
      601 ttaaaaatga gatttatgat agagaacaaa tgataaaatt attagattta gcaaaaaata
      661 cagaaatgga attacctatt aatttggcta ttggattagg tcttagaatt tcagaagttt
      721 taggtctaac ttgggataat attgattttg aagaaaatac aataacagta aataagataa
      781 cttcccgaat taatggttct gttattctca aagagccaaa gacagaaagc tctgttagaa
      841 aaatatcttc tcccaaagaa ttaatgtctt tattaaaaga atataaaata aaacagaata
      901 aaaatttatt aaaatcttcc attagaaata ataataattt gttatttttc aataagaaat
      961 gtgaacctat tgctgaagat gttatgagta aaaaatttaa aagattttta gaaaagaatg
     1021 aattacctca tattagattc catgatttaa gacactcaca tgttacttta cttataaatt
     1081 ctaaagtacc tataaaggtt atatccgaga gagtaggaca ctcaaatata agtactactc
     1141 taagcgttta ttctcatgta cttaaagaaa tggataaaga ggcttctgac aaaatttctg
     1201 aaagtttatt taatgctaat taatattaat ttatcttagc tttaaattag cttagcttat
     1261 tgagatagat tcattaatat taaatgtatc tatctcaaat taataactac ttaaataatc
     1321 tgttttattt taatttatcg cctcagttat tatacttttt tacgaaaaat tatactttac
     1381 aataaaaaag atattccctt atagaaaata tctttttaaa tactatatta ttatatactt
     1441 gctaacattg gaatatctaa catcttcttc attacatcat ctactttttt tgctttatat
     1501 tcattgtatc caattaaata tcctttttca tatgaacttt ttaaaatctc tttttcatat
     1561 aaattatcag atttggcttc tgtgacactt aaattataaa gccctttaca cccatcttca
     1621 taacctttat tatacccttt ttctgcaaaa tagtttttat gatttttttc gttatgtact
     1681 gaagatattg ttactccaga attttctttt gtactagcat aaggacaaaa acctttattg
     1741 tgttgatgtg ctgaatgtcc tccacaataa taacgtgtta actctgattt atttttatta
     1801 tgatgacctc ctgatgaatt atgccctcct gagtgagcat atgaaaaatt ccctacacta
     1861 atcatagcta aactaattac tacagctact aaccttttct taaccataat ctattccccc
     1921 caaaatgtta tatattttta ttatatcatc tactatatta tatttaaatt cttaatatac
     1981 aaaatattac tctattctgc taataaacat gtttaaacaa tataaaaaac ctcctaatat
     2041 ttgattaaaa atttacacac tcatgcatag catgtctcaa atattaaaag gttttatact
     2101 aataattatt tatcttttcc atacatttta gctaattggt cttgtatctc tttactttct
     2161 aaatactcat caaagtccat ttttctatcc attattccat ttggagttat ctctataatt
     2221 ctatttgcta tagtagatat aaattcatgg tcatgagaag taaataaaag cactcctggg
     2281 aacttttcaa gtcctttatt tacagaagtt atactttcta ggtctaagtg gttagtaggg
     2341 tcatctaata caagtacatt tgcatttgaa agcattagct tactaagcat acatctaacc
     2401 ttttctcctc cagatataac ttgagcttct tttaatgctt cttcacctga gaataacatt
     2461 ctacctaaaa atcctcttat aaaactttca cttttttctt cagagaactg tctaagccag
     2521 tcaaccaaag aatattctac tccgtcaaaa aacttattat gattttttgg aagataatct
     2581 tgagaagtag ttactcccca tttatactct ccactgtctg gttcaagttc tcccataact
     2641 atgtttaaaa gtgcagtagt agctatttca tctcccataa atactacttt atcatctcta
     2701 tccaatctaa atgacacatt atcaagaacc ttaaccccat ctatagtttt agttaaattg
     2761 tgtacttcta aaacttcatt cccaatttct cttgcaggtg taaatcctac atatggatat
     2821 cttcttcttg atggttgtat atcttcaact tctaatttct ctaactgttt ctttcttgat
     2881 gttgcttgtt tagcctttga agcattagaa ctaaatctag ctataaattc ctttaattga
     2941 gctatctttt cttcagtctt tttattttgg tccttagcca attgtaaagc caattgactt
     3001 gactcatacc agaaatcata gtttccaacg tacatttgta ttttaccaaa atctacgtca
     3061 actatattag tacatatttg atttaggaaa tgtctatcat gtgatactat tataactata
     3121 gattcttcta aatccattat aaaattattt aaccagttta ttgatttaaa atctaagtgg
     3181 tttgtaggtt cgtccattaa tagtatttca ggcttaccaa aaagtgattg tgctaacaat
     3241 accttaacct tttccccacc aactagttcc ttcatttgtt tgtaatgcat atctttcgtt
     3301 atgccaagac ccataagtat tttctcagca ttagtttctg catcccatcc gtcaagctct
     3361 gcaaactcac cttcaagttc ggcagctttt atcccatctt cttcattaaa gtcttccttc
     3421 atatacaatg catctttttc tttcattatg ttccaaagtc tctcatgacc tcttataaca
     3481 acatttaaaa cagtttcttc ttcatattca aagtggtcct gttttaaaac tgacattctt
     3541 tctttatcag ttatagatat actacctgta ttaggctcta tttctcctga taatattttt
     3601 aaaaaagtag attttcccgc accattagct cctattattc catagcaatt acctttagtg
     3661 aattttaagt taacatcttt aaatagttcc ttatcaccaa atctaagtcc aacacctgta
     3721 acttgtaaca ttaatatatt ttcctttctt tttgctcata atttatttta tcataaaact
     3781 ttgataacat ttttattata tattatcacg taaatagtat attttttata ctaaatttgt
     3841 gaaatattta tta
//
LOCUS       DAYASV010000221         3780 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYASV010000221 DAYASV010000000
VERSION     DAYASV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.226"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="ACGI68_003652"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="ACGI68_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFH0140054.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="ACGI68_003653"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="ACGI68_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFH0140055.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="ACGI68_003654"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="ACGI68_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFH0140056.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI68_003655"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="ACGI68_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFH0140057.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI68_003656"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="ACGI68_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFH0140058.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI68_003657"
     CDS             2662..3168
                     /gene="rplJ"
                     /locus_tag="ACGI68_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFH0140059.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI68_003658"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="ACGI68_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFH0140060.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1531 a          452 c          747 g         1050 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactaa ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
//
LOCUS       DAYASV010000222         3761 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYASV010000222 DAYASV010000000
VERSION     DAYASV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.227"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..1376
                     /locus_tag="ACGI68_003659"
     CDS             <1..1376
                     /locus_tag="ACGI68_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFH0140061.1"
                     /translation="DDFYISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNI
                     LEESSINSKSDVLVVATKEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEI
                     YSVLENLYEFLVDYKKVKSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKI
                     ALATAVAGFIMDYFHLRDGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVL
                     FSIIKDSTLRSLIVLLAGYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISY
                     VALGAIIALIVNKFILPYDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNL
                     LLIPSLIEDRLMLINAIYKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKIL
                     RDTNYISNYNVDKYDEGRSVILESIINTKSLGDKIICLNLLQTLNGVKEMYRISNITK
                     VSIKEAA"
     gene            1591..2448
                     /locus_tag="ACGI68_003660"
     CDS             1591..2448
                     /locus_tag="ACGI68_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0140062.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            2545..3414
                     /locus_tag="ACGI68_003661"
     CDS             2545..3414
                     /locus_tag="ACGI68_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0140063.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         1602 a          335 c          616 g         1208 t
ORIGIN      
        1 aagatgactt ttatatcagt attagaggta tagacataat gaatatttta ttttcattag
       61 aaagaataag tttaatccta gaaagataca aaaaggatag taaagaattt gaagatgaag
      121 atataaaaaa tattttagaa gaaagtagca taaatagtaa atcagatgtt ctagttgtag
      181 caacgaaaga aataaactat attaagatgt gtttagaaaa taaagatacc ataactgata
      241 aagagatatt aggatttaga gattatgtta ttgctcagga tactaaaaat ataaatttaa
      301 aagaaattta tagtgtttta gaaaatttat atgagttttt agtagattat aaaaaagtaa
      361 aatcaagaga cgaaaagaaa gctgaaagaa aatcaaagat acctcatgaa tttaaacgac
      421 tatcaatata taagaaaaac tttaatttaa attctataag attttcttat gcagtaaaga
      481 ttgctttagc tacagctgta gcgggtttta ttatggatta tttccattta agagatggaa
      541 gatggataat gttaactgtc ttttcattaa cacaacctta cgcagaaaat tgtatacaaa
      601 ggtctagaaa aagaatagaa ggtacattta taggagctgt aatatttatt gtattatttt
      661 caataataaa agatagtaca ttaaggtcgt taatagttct tttagcaggg tatataaact
      721 cttatgtagt tgattataga aaattaatgg tatgtgtaac tgtttcagct cttggttcag
      781 cagtagttat gggagaccca aatgttttaa ccataagcag gatttcatat gtagcattag
      841 gagcaattat tgcattaata gtaaataagt ttatattacc atatgatgct aaaacaggct
      901 atcaacatgt tatagaaatg tataaaggaa tagttaaaaa tataattgat gaagtcaata
      961 agtctataga aaatgtagct gatgtttact atataaaaaa tttattattg ataccatcac
     1021 ttattgaaga tagacttatg ttgataaatg ctatatataa agataaacat caagaggatt
     1081 ttttagaaaa tcagaaatta ctaataagta atatgtataa tctatatata aatgttaata
     1141 aaaataaaat aaaagatgaa gatgttgaaa aaatattgag agatactaat tatatttcaa
     1201 attacaatgt agataagtat gatgaaggaa gaagtgtaat tttagagagt attataaata
     1261 ctaaaagtct aggtgacaag ataatatgtc taaacttact gcaaactcta aatggtgtca
     1321 aagaaatgta tagaataagt aatataacta aagtttcaat aaaagaagca gcttagatat
     1381 taaattatag aatagccata tatttaaaat aaagtatatg gctatttgtt ttataaaata
     1441 ctaaaagaaa ataatatata aaaaacaaaa aacatatatt tatgaatttt atatgaaaat
     1501 agttaaaata ttgatttttt tcattggttg aaaaaacaat ttacttagga taaactataa
     1561 tagtattcaa atattatggg ggttagattt atgagtaaag atgtaaaggt aaagcgtaat
     1621 aagaaatatg atacatttag tataactatg gaacttgaga gacctttact ttatgatgag
     1681 gttgagaaca aagatgataa aaataataat agaaaattta acataaagaa taagatattt
     1741 aagggaatag gtatactatt tgtattgatt ttgcttggct ttttcatatg gataaataaa
     1801 acatatgaac ctcaaaagct ggcaaaagaa gctctagtca gcaatagtaa agttgaagta
     1861 actgtaaatg aaaacatttc atttacacca aaaggaagaa aagtttctaa aggtttaatt
     1921 ttgtatccag gagctaaagt agaaatagaa tcttatgctc cacttgcaag aaaaatagca
     1981 gaaagtggat atgaagttgt aatcgtaaag atgccactta atctagctat acttggaact
     2041 aataaggcac agaaagttat ggattcttac aacaacatag aacattgggt aataggaggt
     2101 cattcattag gaggagttgc agcatcaaat tttgcacgtg ataataaatt aattgaaggc
     2161 gttgtatttt tagcttctta tccaatgggt gatgagttga aagaattagg aaaaaaagtt
     2221 atatctatat gggggagcaa agatggagta gtcaatttta aaagtcttgt agaatcaaaa
     2281 caaaaacttc ctgatgatac aacttatgtt gagattgaag gaggaaatca tgcacaattt
     2341 ggagattatg gaaaacaaaa gggagataat gatgctatta taagtcaaga aaaacaactt
     2401 aatattactg ccaatagtat cattaaattt ttaaagaata tatcttaaac taaaatttaa
     2461 aaaatataat tttataagtt attatttaaa gtaaatatta ataatataat agaatgaata
     2521 ttttattaca agggggattt taatatggaa tacgatatgc cactttacag accaccaagt
     2581 gaggcttata gtcttataat acaagtcaca ctaggatgtt ctcataacaa atgtacgttt
     2641 tgtagtatgt acaaatctaa aaaatttaaa ataaagccac tagaagtaat aaaaaatgaa
     2701 attgatatat ttagaagata ttataagcat gtagaaagaa tattcttagc agatggagat
     2761 gcattgataa taccaatgga gaaattaaga gatattattt tatatataaa agaagttttt
     2821 ccagagtgtg aaagaattac tatgtacggc tctcctaaat ctatagagaa aaaatctgat
     2881 gatgaactta aagaattaag aaaacttggt gttaaaatga tttatctagg gcttgaaagt
     2941 ggaagcaatg aagtattaga agatattaag aaaggtttta gtagtgaaca attaataaaa
     3001 gttggtagaa aagttaaaaa aagtgggatt aaattatcag ccactgtaat agcaggtctt
     3061 ggaggaacta aaaaaacaca tcagcatgca gttgatacag gaaaaatgct aggagcaatt
     3121 tcaccagatt atgtgggtgt acttagttta atggtagaac ctaatactga actttacgat
     3181 ttacttaagg caggagagtt tacaatacta gaggataagg ctgtgttaca agaaattaaa
     3241 gagatgataa agaatataga tgctaatgaa aaaattgtat ttagaagtaa tcatgcatca
     3301 aattatgcta atttaaaagg tgtattacct gatgataaac aacgtctaat tgatgaaatt
     3361 gattactatc taagtaacca aaagctaaaa agagaagaat atagaagact atagtttttg
     3421 atatatcaat ctatactgat aggtaaatac taagattatt tagatacaaa aaatcatgct
     3481 acattaatag ttgcttacta ttgatgtagc atgatttttt attaaaaaac aaaatggtat
     3541 attcatatat aatatatcaa taataatgaa aatgtgaact agtttaatag aattttacct
     3601 attataataa aaacaggaat ttaaataata aaaagtaaag taacttttta ttaagtaact
     3661 ttacttttta ttttatttaa tgttgtatta aatttatata tattaaaata atatacaaat
     3721 tttgaaaaag ctcttataaa atgattatta aagtaaagtt t
//
LOCUS       DAYASV010000223         3661 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYASV010000223 DAYASV010000000
VERSION     DAYASV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.228"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..674
                     /locus_tag="ACGI68_003662"
     CDS             <1..674
                     /locus_tag="ACGI68_003662"
                     /inference="COORDINATES: protein motif:HMM:NF014543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFH0140064.1"
                     /translation="FMENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKS
                     RNGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKK
                     NGFGIYIFSSKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNND
                     IYKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNN
                     NKLKV"
     gene            833..1339
                     /locus_tag="ACGI68_003663"
     CDS             833..1339
                     /locus_tag="ACGI68_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFH0140065.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1467..2483
                     /locus_tag="ACGI68_003664"
     CDS             1467..2483
                     /locus_tag="ACGI68_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFH0140066.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTPANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     regulatory      2679..2773
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            2837..3541
                     /locus_tag="ACGI68_003665"
     CDS             2837..3541
                     /locus_tag="ACGI68_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HFH0140067.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1568 a          316 c          647 g         1130 t
ORIGIN      
        1 tttttatgga aaatataact agttatttta caacaatact atgtgtattt atatgcttat
       61 catctgtatt tatatttact caactagcta gagtatttat taataagaag aagattaatc
      121 aaaaaattaa atcaagaaac ggatttaggt atgatagaga ttttattgaa gccagaagag
      181 aagaaataca tataaaggat aacaataata ataaaaataa gagtaacaat aagaaattaa
      241 agggagaaaa agtatttaaa tacgataatg gtgatttata taaaggtgag tttgttgatg
      301 gaaagaaaaa tggatttggg atatatattt tttcaagtaa ggaaaagtat gaaggattat
      361 ggaaagatga caagatgcat ggaataggaa aatatactta tagagatggt tcaatatata
      421 caggtgaatt taaatacgga ttaaaaaatg gtttgggaaa attaacatat cccaataatg
      481 acatttataa gggatatttt ttagataata agaaaaatgg taagggtgta ttatataaaa
      541 atgatggaaa taaacaagca ggaatatggg aagatgatga acaatgtaaa agtttagatt
      601 ttaaagattt aaataataat aaaataaata atgtttatca aaatagatta aataacaata
      661 aattaaaagt ttaattattc taataaaaaa atctttattt atgatggaat ataggatata
      721 tctatgttgt aatttgtaaa atcttcctat atcatataaa attaagtatt gattagtcta
      781 ttacataaat atagaatata aatatatcca tttaataata aggagaagag atatgaaaga
      841 gtatgtagtt gtggatttag aaactacagg attagatcca tataaaggtt gtgaaataat
      901 agaaatagga ataacagaaa taaaaaatga acaaattgta aaaaactatt caaggttgat
      961 aaaacctaag gggataattt cttcatttat aacagaattg acacatataa gtaatgaaat
     1021 ggtagaaaat gaagaaccac tagaattagt tttacctaga tttagaaaat acataggtga
     1081 tagaactata attgcacaca atgcgaaatt tgatttaaaa tttttaaatt actatcttag
     1141 aatgttagat ttagaaccaa taaacaatta tatatgtaca gtagagttat taaaaaaatg
     1201 taaaagttat aaaggaaaaa acaagaaact tgaaacagct tgtgcttatt acaacataga
     1261 gaatataaat gctcatagag ctgacagtga tacattagca actgcaaaac tatttttgaa
     1321 aataaaagat gaatattaat tataaatata gagaaaatta gtgaaaatat cctttataaa
     1381 attgaccctt aagcttacaa ttttgttctt tgatttattt tgtagaacaa tgtataatgt
     1441 accttatatg aagaagggag actaaaatgg gttattacaa tgataatgat aataataaaa
     1501 taagacgtaa aaaaattagt aacagcaaag atagtaataa aaaatctaaa cattcttata
     1561 ctccggcaaa taaaaaaaat aaaagttcta agacaagttc tagtaaaaga atgagtgaaa
     1621 aagtagattc acagaatctt ataaaaagag atagagatag ttttacaaga gataatgtaa
     1681 gacctttaaa tacagattac aatcaaagag aaagaagaag agtcgagtat aaacaaagag
     1741 agaaaaaatt aaaaagtaaa aaaagaaaaa aacaaaaaag gattagaata gttagagcat
     1801 tattatctat tatgttagtt gcactaatta tattttcagt ggcaggtgca atatttacag
     1861 tttcagctat aaaagggtca ccagaagtta ctaaaaagtt aattaggtct aaatatataa
     1921 gtagtgaggt tgtaagtata aaacaaatgc caaatgattt aaaacatgca atagtttcca
     1981 tagaagatga gagattttat aagcataaag gagtagatgt tatttctcta gttaggtcag
     2041 tattaaacaa tgtgtttaca gatacaacac aaggtgcaag tactatagat atgcaagttt
     2101 caaaaaacct gcttactagt aatgataaaa ctatgaagcg gaaaataagg gatatgtata
     2161 atgctaagca gatgaataag attatgacta aggatgaaat tcttgaagca tatttaaata
     2221 atatgtatct aggtaaaaat gcatatggag ctgcaaaggg agctgaagtc tactttggta
     2281 aaaaagttag tgaattaaat ttagctgagt gtgctatgtt agctggtatg acaaataatc
     2341 ctgcaaggta tattgaccat ggagaggcta agaagagaca agtgattgta ctgtataaga
     2401 tgcatcagct tggttatata actgataagg agtatagaat agcaaaagct gatgacactc
     2461 catttaaatc agaaattgat taaaattgta agaatatagt attttcaata gtttaaacaa
     2521 acaaaaatgt aataaaaaag gtgatttgcc acccatttgc caccataatt ataatctaat
     2581 ggcaaattgt tttaatgtac aaactagata atagaatatt gtcttaaatg ctatattatg
     2641 ctaaaattta attgtatttt gtaaattaaa aaacttataa tttagttttc tagggttccg
     2701 cattttataa tggtcaggtc caagagaaaa cgcgcaattg ttgtgtacac ggaaggataa
     2761 aagcctggga gatatttaag aatatttcta taggcttttt tatttttaca tattttaaat
     2821 ttgaggaggt tttctaatga aaaaatttgt tattgaggat gcattttgga atctatttcc
     2881 tagtgcaaaa attggagttg ttatttgtaa tgatatagat aactctataa aagatgaaga
     2941 ctataagaaa ataattttga agggagaaga agaagcatta aaatacttgg aggactcaga
     3001 atttagcaac aaccaagtga ttaaagtatg gagagaagca tttaaaaaat ttaaaacgaa
     3061 aaaaggagca aggtcttcta ttgaagcatt attaaaaaga gttcataatg gaaataatat
     3121 aggaactatt aaccctttag tggatatcta caattttatc tcattaaaat atgcattacc
     3181 ttgtggaggg gaggatatag ataaatttat aggagatatt aggctaacta gagcaattgg
     3241 taatgaggag tttatcccac tagggacaga tgaaaattca tttccatatg aaggagaaat
     3301 aatttataaa gatgatggtg gagcaatttg tagatgttgg aattggcgtg aagcagtgag
     3361 aacaatgctt actgaagata ctaacaatgc ctttttatgc attgaattag tagatgaaag
     3421 tagatttaaa gaatttgaaa atgctttaaa tgaattagct aaaatagttc aagaaaaatt
     3481 gggtgggaat tgtaaaattt caattttaga tattgataat aaagaggtat cagtggatta
     3541 atatggaggt gctgttgtaa tggactattc tagttataaa gatacttata tttagggtaa
     3601 gaaggtctat tttagatata acgattatca aaggatataa tatagaaaat aaaaatgtag
     3661 a
//
LOCUS       DAYASV010000224         3644 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYASV010000224 DAYASV010000000
VERSION     DAYASV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.229"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            113..1489
                     /locus_tag="ACGI68_003666"
     CDS             113..1489
                     /locus_tag="ACGI68_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HFH0140068.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1800..2471
                     /locus_tag="ACGI68_003667"
     CDS             1800..2471
                     /locus_tag="ACGI68_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFH0140069.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2660..3457
                     /locus_tag="ACGI68_003668"
     CDS             2660..3457
                     /locus_tag="ACGI68_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFH0140070.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         1378 a          400 c          678 g         1188 t
ORIGIN      
        1 tttatacaat atttatacta taatagaata ataatttaga ggaaagctct aaaaataaac
       61 aaattaaggg tcttaacagt agtgatttta tgttaaggag gtataaagaa agttgaaaac
      121 aatagttaga agacttattc tctacgcaga tggaatgcgt cctactcaaa taatggtatc
      181 tgggtttgct gcgattattg taatcggagc attgttgtta acactcccga tagcgtcgca
      241 gagtggagag agtataggct tattaaatgc tctatttacg gctacttcag cggtttgtgt
      301 tactggtcta gttatggtag atacagcaac ttattggagt ttatttggac agatagttat
      361 aataacattg atacaaattg gtggtcttgg atttatgacc gtagccacaa tgttttcact
      421 tatggcacga aaaaaaatac aactaagaga aagattatta atacaagagt cattaaatca
      481 agctgattta tcgggacttg taagacttac aagatttgta ttaataataa ccataacaat
      541 agagggaata ggtgcactgg ttttatcaac tgtctttatt ccacaatttg gtttgtctaa
      601 agggatatgg tatagtgtat ttcacgcaat atcagcgttt tgtaatgccg gatttgactt
      661 gatgggcagt gtaagtggcc catttacatc gttaaattct tatgtaaaca actttacagt
      721 atcaatgact gtttgcgcac ttatagtgct tggagggcta ggattccctg ttgtacttga
      781 tattgtaaga aaaagaagat tttcaaaatt aaatgtgcat tctaaggttg tattgttttc
      841 aactgctaca cttatttttg tgggtgcatt atttattttt cttatagaat ttaatcaaaa
      901 agctactatg gcggacttgc ctttaaaggg aaaagtacta tcagcaatat ttcaatcagt
      961 aacagcaaga actgctggtt ttaatacgct tgatttagct actttacgag aaagtagtgt
     1021 atttgtaatg ataattttga tgtttattgg agcatcacct gcatcaactg gtgggggtat
     1081 aaaaacaaca acactagctg ttttaatcat tacagttaga agttttctat caggaaaatc
     1141 agatatagaa gcttttgaaa gaagattagc tccatcaact ataaaaaaat ctcttggtat
     1201 atttgtaatc agtatatcag cagttatttt tggaacattg attatttcaa taactcagcc
     1261 taattttaca ttagttcaat cagcttttga ggttacatca gcacttgcta cagttggttc
     1321 aagtttagct ggaactccaa acttaaatgc tttaggtaag attataataa taatctttat
     1381 gtttatgggt agagttggtt ctcttacttt atttatggct atactttctg gtggtagaag
     1441 aaagagtcaa ccaatcagat atgcagaagg taaaattatg gttggttaag ttaccaagga
     1501 gtagtataaa tttataatgg gtttagagta gcactgtaaa tctgttgata aaataaatga
     1561 gcctagatgt aattatatga agaaataaaa tcaactaatg aacaaaatta attaatgaaa
     1621 taaaaaaaca gggaaataaa atacaaataa aaaggtaaat caaaattaaa ttaatctgtg
     1681 tgtataaaat tatatattgt gtataagttt tgtatgtgta aagtatataa gttttgtatg
     1741 tgtaaacaat taatttaaga gatattatat aaattgtgta ttagggagga agtttcgaga
     1801 tgaagcaata tatagtcatt gggtgtggga gatttggaag ttcagttgcg tctactatgc
     1861 atcttttagg acatcaagta atggcaatag acaaaaatga agattcagtt caaagtatat
     1921 ctgacaaggt aacccattca cttatagtgg atgttactga tgagcaagcg ttaaggtcat
     1981 taggtttagg taactttgat gtagcagtag ttgcaatagg ttctgatata agggcatcta
     2041 taatggcgac tcttatagcc aaagaaatgg gtgtagagtt gataatatgt aaggcaaagg
     2101 atgaattaca agctaaagtt ctttataaaa ttggtgcaga tagagttgta tttccagaaa
     2161 gagatatggg agtaagagtt gcacacaatt tagtttcgga taatatatta gaccatattg
     2221 aacttgaccc agagtattca attgttgaaa tcgtaactcc aaatagttgg gttggcaaga
     2281 cacttataga gcttgaatta agagctagat atgagataac tgtacttgct ataaaaacag
     2341 gtaaaaatat aaatgttaca ccttctccag atgaggaact tacagccgga agtatcctag
     2401 ttataatcgg tcaaaatact agtataacag cgataacatc tggaaataag gggataatta
     2461 gaagaagata atttactatt taatatatat ttaattgcaa tgaaagcaga tagtatcata
     2521 taattatgaa tagttatatg atactatttt tttaatcgaa ggtagtattt atttgtaaga
     2581 ttagataaag agaagttaaa ttaaaagtaa ggaggctgtg ctaatataaa aatttataag
     2641 ttattagcat ttagataaaa tgattacaaa tataaacagt aaggataatg aaaagttaaa
     2701 gtatacaaga gcactattaa aatcaaagaa taggaataaa gagtcaaagt tcataataga
     2761 aggatacaga atagtaatgc ttgcacttga atgtatggca aaccttgatt atgtatttat
     2821 caatgaagaa tttgaaaata agaaagaaca tgtaaaacta ttagaagatt tggataaaaa
     2881 aaacacaaag atatacaaga ctactaataa aaactttaaa gaattagtgg atacagaaaa
     2941 tactcaagga ataataggtg tagtttcatt taagaaaaaa aaattaagtg aaagtataaa
     3001 taaaaaagat aaatttgtat tgattttaga cagaatacaa gacccaggaa atatggggac
     3061 tataataagg actgctgatt ctgctggagt agatgccata atagcactaa agggatgtgt
     3121 cgatatatac aacccaaaag taattaggtc tactatgggt tctatttttg atatgaatat
     3181 aattgatgct tcacaagatg aaactgtgga catgcttaaa tcattggatt ttaatatagt
     3241 ttcaagttac ttaaatacag aaaattttta tgacaaaata gattatggct caaaagtagc
     3301 attggtgata ggaaacgaag caaatggaat aaatgaagaa cttgtatcaa agtctgatat
     3361 tttggttaag atacctatat atggtaaagc cgagtcgtta aatgctgcga taagctctgc
     3421 tatactgatg tatgaaataa aaaaatactt aatttaatgt attgtaataa atataatgtc
     3481 atgttataat cttgaattaa atagtagagt atcaaaaata gtcaatatat attaaaaaaa
     3541 taattaaatt aattatatat tagatgtata ttatataaaa aataattgag ttaattatgc
     3601 attagatgta tattatataa aaaataattg agttaattat gcat
//
LOCUS       DAYASV010000225         3632 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYASV010000225 DAYASV010000000
VERSION     DAYASV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.230"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            156..1736
                     /locus_tag="ACGI68_003669"
     CDS             156..1736
                     /locus_tag="ACGI68_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFH0140071.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1910..2356
                     /locus_tag="ACGI68_003670"
     CDS             1910..2356
                     /locus_tag="ACGI68_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFH0140072.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2377..3420
                     /locus_tag="ACGI68_003671"
     CDS             2377..3420
                     /locus_tag="ACGI68_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFH0140073.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1457 a          335 c          691 g         1149 t
ORIGIN      
        1 aattgtttta attcaaaatt ttttgttatt ttaaattatt tcaaaactct tagttttagt
       61 gattttattt atgatattta tgggattaaa aagatttcaa tagatatttt tggtaagtat
      121 ttaagaagtc tttaacttta taggagtgat aatttatgct aataggtaca tctaaattaa
      181 gcagaaatgt tgatactgca tgtgtagaag aagtagggat tccaatgatt gtattgatgg
      241 aaaatgcggt tatatctgca atgaaaaata tggatataga catgtataat agttatacag
      301 ttgtatgtgg tgttggaaat aatggaggag atggtcttgg aatagcaaga catctaaata
      361 catatggaaa agaagtaaac gtatttttag taggtaatct tgaaaaacta agtgaatgct
      421 ctaaaataaa ttacaatgta cttttaaata tgggaattaa tattataaat attaataaaa
      481 atgatttaag tgaaaaaaat tatggagagg aaaatgaagt agataaaatt attaaagttg
      541 agaaacaaaa tattataaaa aataaaaatt taacaatttt taaagataga gtatctaaaa
      601 gtgatgttgt agtagatgct atatttggga ctggattaaa acgtgatata actggtattt
      661 ttaaagaagt tattgacata gtaaacgata atagcaaaaa tacgtattct atagatattc
      721 cttcaggtat agatagcgat aatggaaatg tattaggaac ttgtattaga gcaaacaaaa
      781 cgattagctt tgagttttat aaaagaggtt ttctaaacta tgacactagc gcacttatag
      841 gtgatgtagt tgtagaaaag ataggtgttc cagattttat tacaagaaaa taccatgatg
      901 aagagttcat aactgataag acttttgtaa aaaataatat tagaagaaga gataagcatg
      961 gttttaaaag tgattttgga aaagtatcta tagttgcagg ttcaaaagga ttttatggtg
     1021 caagtttcat agcaactgaa tctgctgtta agagtgggag tggacttgta acattaatta
     1081 gtagtgaaga tgtgcaagaa aaggtatcta ttagacttac agaagcaatg actttaaatt
     1141 ttgaagaaga aagattaaat aaattactta attctagtga tgcaattggt tttggtccag
     1201 gtatgggaga taatagtcaa acatttgata aattacttaa gatagtagaa aattctaatt
     1261 gcccaatagt attagatgca gatggattga acgttatgaa ggacagatgt tataagtttt
     1321 tagaatggaa aaatagattt gtaattacac ctcatttggg agaaatggcc aggttgacag
     1381 gagattcaat tgggtatata agagaacata gagtagatgt tgcaaaagaa tttgctcaaa
     1441 aatataatct ggtggtgctt ttaaaaggtt atcaaactgt cataacagat ggtaaaaaaa
     1501 catatataaa tcctacagga aatagctgta tggcaactgg tggaatgggt gattgtttgc
     1561 ttggaatgat tacttcattt ataggtcaag gtatggatat attggaagct acagttagtg
     1621 gagcatatat acatggctat ataggagata aattgagcaa agcaatgtat acggtaaatg
     1681 ctacggattt aataagtaac atttcattaa caatgaaaga gttgttggat atataattgg
     1741 tgatatttga tactactttg taacaatttt aacaaagtat gttgatataa tactattaat
     1801 taattaattt attttattgt tttgtaattt aaatttatat tcaaaaacat tgtataaaca
     1861 tataatttat atagggtttt ataattcata tgaaaagagg gtagatagta tgaagctaat
     1921 atggttgata gtagcagtct tatttggaat agcagaaatg ttaacaccaa gcttaacatt
     1981 gatatggttt agtgtaggag ctgtaatact tatattttta agtagcttta tagagagtat
     2041 tttcttacaa attttaatat ttgcagtaat ttctatagca atgttagtcg tagctactaa
     2101 aaaaatagta aaaaaagata aaggctataa atctaacact aatttacaag caatgatgtc
     2161 taaaaaaggt attgttacag aagagatttc tccaaataac acaggtctag ttgtagttga
     2221 acatgaaaca tggacggcta tttctataga tggtgaaaag atagaaaaag gttctacagt
     2281 agaagtatta aaaattgaag gggtaaaatt agttgttaaa aaagtagacg ccacagttaa
     2341 tgtgacaaac caatagaata aaggaggatt tatagtatgg gtactaaaat tgttttaagt
     2401 attgtattaa tagtagtagt cgtagcaata agtttaactt gtataagagt tataaaacaa
     2461 tctaaagtag gtataataat gagacttggt aagtttcaaa aagtggctga aacaggtgta
     2521 catttcttaa taccattttt agataaaatg gcatatgtaa ttgaccttag agaaattgta
     2581 atagattttc cacctcaacc agttataact aaagataatg taactatgca gattgataca
     2641 gttgtatatt ataaagttac agacccagtt agatatgtgt ttgagatagc taatccaata
     2701 gctgctattg aaaatttaac agctacaaca ctaagaaata taattggtga acttgattta
     2761 gatgaaacat taacatcaag agatataata aatgtaaaaa tgagaactat tcttgatgaa
     2821 gcaacagata aatggggtat aaaagtaaat agagtagagt taaaaaatat aatgcctcct
     2881 caggatattc aagttgcaat ggaaaaacaa atgagagcag aaagagaaag aagagaggca
     2941 atacttcaag cagaaggtaa taagtcagct gcaatacttc aagctgaagg tgaaaaacaa
     3001 tctgctatat taacagcaga agccaaaaaa gaagctatgg tacgtgtagc agaaggtgaa
     3061 aaagaatctg ctatattagt ggcagaaggt gaagctgaag ctataagaca gacagctatt
     3121 gctaaggcac aaggtgaagc tgaaatgata aaaagaactc aaatggcaac agcagaaggt
     3181 ttaaaattag ttttttcagc aatgaaagag gctgatatag acaataatat tctagcatta
     3241 aaatctatgg aagctcttga aaaaatggct gaaggtaagt caacaaaact ggttttacct
     3301 tcagaagcag ttaatttcct aggaacattc aaaggaataa aggaagttat gagtgatgat
     3361 aacaaagaag tacttgatat aaaagaagtt ttaaatgata atgaatcatt aaaaaaataa
     3421 aagagttttg ttatcatagg aaggatatgg aaattaaagg tcgaattaat taaatatagc
     3481 acaaaaatgc acgttgaaaa gaagaggact tataaatttt taagtcttct tctttgtttt
     3541 tattagaaag atgaaattat aaaaataaat taacagaatt tttatacaat ttatccttta
     3601 ctaatgtaaa aagtgttata taatattata ta
//
LOCUS       DAYASV010000226         3612 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYASV010000226 DAYASV010000000
VERSION     DAYASV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.231"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            26..679
                     /locus_tag="ACGI68_003672"
     CDS             26..679
                     /locus_tag="ACGI68_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0140074.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            820..2091
                     /gene="brnQ"
                     /locus_tag="ACGI68_003673"
     CDS             820..2091
                     /gene="brnQ"
                     /locus_tag="ACGI68_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0140075.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2293..3480
                     /locus_tag="ACGI68_003674"
     CDS             2293..3480
                     /locus_tag="ACGI68_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HFH0140076.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
BASE COUNT         1316 a          400 c          668 g         1228 t
ORIGIN      
        1 aatataattt atagtggagg ggaaaatgac taaaattatt aaagtgcaaa aacctaaatt
       61 tacaggagaa ttagaaatta ttgaaaatct tgaagatata cttgaagata tatttaatga
      121 tgaaaaaata tttaataaaa ttatagaaaa tgaagttata agtgatttag attctgtaag
      181 agttagcttt gaatcttgta tatttagaaa tgtgtctttt gaaaattgta atttaaaaaa
      241 gattgattta cttgatgtgg tatttgaaaa ctgcaattta tcaaatacat tttttgatgg
      301 aggaagtata tacagagttg aatttaaaaa tagtaaattg ataggagtta gatttgatga
      361 ttgtatactt aaaaacgttt tgttcaagga tgttttaggt aagtattcta acttttcatt
      421 ctctaaattt aagatagttt ctatcttaga ttcaaatttt gaaaattctg tatttcaaga
      481 agtaaaaggt gacaatctag tatttgagta tacaaattta aaaaaagcaa actttaatgg
      541 aactaaactt tctggcatag attttactac taataatatt gagggaattg agattggtat
      601 agatgatatt agaggagcaa tttttgatgt gtcacaagca attgatttaa cgaaattgat
      661 gggaattatc ataaaatgaa aaaaatattc ataaaataaa ttgggaaaac gttaacaatg
      721 tgtgaatttt taccagattg acatttttct gaaaataaag taagattatc ttaatattca
      781 aaatattttc aaagggggta atttagtgtg agtaagcaga tgagagatat ttttatagtt
      841 ggttttgctt tattttctat gttctttggt gcaggaaatc ttatgtttcc tccattttta
      901 gggatggaat ccggcaaaga ctggttaatt cctcttattg gatttgttgt tgcagatgct
      961 ggtatagcaa tgcttgttat tatagcaaca gcaagatgta agggaagcat ggatgatgta
     1021 cttaggagag ctggtaaagg gttagcaaga gtaatgagtg tagctgctct agcatgttta
     1081 gcacttcttg taataccaag aacttgcgca acaacatatg aaatgggtat tatgcctata
     1141 tttggagacc atggtccagt agctaaagct gtattttcaa taatcttctt tgggctaact
     1201 ttagcattta ctattaggtc ttctaaggtt atagacataa taggtaagta tttaactcca
     1261 gcgttattat tagtattatt tgttttaata gtaaagggta taatttctcc agcaggacct
     1321 atgagtcctg agcatatgat agataaaaat ctatttggtg aaggtatatc acaaggttat
     1381 caaacaatgg acgctctagg tgcagcatct atggcaacta taatgttgat gtctataata
     1441 gctaaaggat atacatcaga taaagaccaa ataggaatga ctattaaagc tggttttgta
     1501 gcctgtgtat ttctagcagt agtatatggt ggtcttactt atttaggtgc aacagtttca
     1561 actttatatg atactagtat acctcaagca tctttattag ttgaaataac tagtatattg
     1621 ttatatggtc aagtaggaaa ggtaatttta ggtgtaattg tggcacttgc ctgtctgact
     1681 acttctacag gtttaacagc atcaatatca acttattttg aaggaataag taaggcgagt
     1741 tataaacaac tggttatagg tatatgtgta gctagtatgc ttatatcaaa tttaggtgtt
     1801 gattctataa tagccatatc agttccaatc ctacaaacta tatatcctgt tttattagca
     1861 atagttgtta tggagttggc tggtaaacat ataaagaatg ataatgcttt taaaggtgca
     1921 gcatatgtta cattagtgat tagtttatta tcagctataa atggaatgac aggagctgtt
     1981 ccatttatac aaagcttgcc acttgcaggt ttgggcttta actgggtaat accagctata
     2041 ttgggtggaa tagttggtaa ctttataaaa agcaacaaac aagtagcata ataatttgta
     2101 attaatttaa ttttataata tattaatttt tagtactatg ggtaggttta atctaaacct
     2161 aatctaccca tagtactttt tagattggat tataatagca tgtatataat tttttataat
     2221 ataatttttc tttgaagtat gataaaatta gtaatagtga taagaatgta caaatttagg
     2281 aggttttaat taatgcttag aaaaataaca gcatttatgc tatccatatg tttaattatt
     2341 ggacttacag gttgtagttt acttgtaggc aagaaagata aagagaaaat tatagatgat
     2401 tttaaagttg cagtggtaac tcaaccatta tctgaaaata aagtacaata caatatggtg
     2461 gaagagatgg caaaagaata tgaggaagag aataaaattg ataaagacaa agatggtcaa
     2521 actaaagtta aacaaactat aaagcatgtt gtattgccag agaattttac ttctaatata
     2581 gacagtgcta ttaataagat agtaaagttg gctgacgata aagaagttca ggctattgta
     2641 gtttcaactg accaagcagg gcttttacct gctttacaaa aggtaaagga aaagcgtcca
     2701 gaaataataa ctatttctgc ccctatggga gatgataaaa accaattaag ccaatttgtt
     2761 gatgtgaatc taggggttag tgcagaggaa agaggaaaag tattagctga acgttcaaaa
     2821 gaaatgggag caaaagcttt tattcattat gcctctacag atgatttgaa agatgttaat
     2881 atagccaaaa gattagaaat gattaaagag acttgcaaaa atataggatt gccatttgta
     2941 caagttaata ctccaaatat aaatactgaa gaagacaaaa acaaagttaa gcagttttta
     3001 aatgaagata ttgaaaaaca agttaaaaaa tatggaaaag atataaatgt atttggtgta
     3061 aatgagtata tggatgaagt tatattgact aaggctttag aattaaagta tatagttgca
     3121 gagcaaagca atccatcacc aatacaaaca tatcctagtg ttatgggtct aaaaatatct
     3181 gaaaaagatg ctcaaaatta tgataaaata aacgacatga taagtgaaaa ggctaaggct
     3241 tttggcatgt caaatagact tggtggatat cctatgccaa tggatgcttt tctaccatca
     3301 gtagcaattt atcttgctac tgaaatggtg aaacaagatt taacacaaga agatgtatgt
     3361 gacccagatt atttagaggc gtttacagaa ttaagatttg gaatcggttc agaatttact
     3421 cctttaacag aggtacttta taattatcaa tcagtaattt taagccaatt aatttattaa
     3481 tattaaggac gtgtctcaaa atgaactttt tagtttatga ggcactcttt ttctgttaaa
     3541 tcaaatatat ttttatcttt ttaacaatta aaaagaagct tatccatatg ttgagatagc
     3601 ttcgttttta at
//
LOCUS       DAYASV010000227         3584 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYASV010000227 DAYASV010000000
VERSION     DAYASV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.232"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(68..1915)
                     /locus_tag="ACGI68_003675"
     CDS             complement(68..1915)
                     /locus_tag="ACGI68_003675"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HFH0140077.1"
                     /translation="MKPILKWAGGKTQLLDILRKQMPTGCDFSSMTYIEPFVGAGALF
                     LYLLEEDIFNKYVINDINSKLINLYIKVRDDLEELITELTNLKEQYISYEEGSSDREK
                     MYYDIRDRFNILTNEKNIENEDCIKQASYFIFLNKTCFNGLYRENSRGEFNVPFGRYK
                     NPSFFDKNELEKLSMLLNKRDSRGELKVKILNKNYLNLENIIDKNCFVYLDPPYRPVT
                     IGGFNSYNKGNFNDDSQIELSKFYKVLDKIDTKLMLSNSDPKVLDEEDTFFDDLYSDF
                     NIKRVHAKRFINSKGNGRGFITELLITNYEEVGNQMELVKNIHKNKSKDEIFKELLEN
                     LKGTIASWDYYVNWNKALENTRKIEVQLNILNYLIGKEDIENETKNLLKEYPEVLSVI
                     PIVIASRDNEFKILDPSDEDMFKYKLFNFKKRKSISDEESELATEFLKKIKFLDLLKQ
                     KNIKNLVDYVFGVEVGLDTNARKNRTGSSMETLVERYVKNMCEKNNWSYLTQATVKKI
                     SEKWSIEVPTDKSKRKYDFAINNNGKIVLVEVNFYNGGGSKLKSVANEFTKLDKYIKD
                     GGFTFCWITDGLGWLESSIPLRDAFDKMDYIFNLDMVDKGSLEYIIKNL"
     gene            complement(2343..2692)
                     /gene="ssrA"
                     /locus_tag="ACGI68_003676"
     tmRNA           complement(2343..2692)
                     /gene="ssrA"
                     /locus_tag="ACGI68_003676"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2850..3305)
                     /gene="smpB"
                     /locus_tag="ACGI68_003677"
     CDS             complement(2850..3305)
                     /gene="smpB"
                     /locus_tag="ACGI68_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFH0140078.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         1180 a          644 c          341 g         1419 t
ORIGIN      
        1 ttatataata tataagtcta aattaacttg caatataaaa gcgcctctta atataggcgc
       61 tataatgtta taaattttta attatatatt ccaatgaacc tttatcaacc atatctaaat
      121 taaatatata atccatctta tcaaatgcat ctcttaatgg aatacttgat tcaagccatc
      181 ctaacccatc tgtaatccaa caaaatgtaa aacctccatc ttttatatac ttatctaact
      241 tagtaaactc atttgcaaca gattttaact tagaacctcc accattataa aaattaactt
      301 ctactaaaac aatttttcca ttattattta tagcaaagtc atactttctt ttactcttat
      361 ctgttggaac ttctatagac catttctcac ttattttttt cactgtagct tgtgttaaat
      421 aactccaatt attcttttca cacatattct ttacatatct ttctacaaga gtttccatac
      481 tacttccagt tctatttttt ctagcattcg tatcaagccc aacctctact ccaaacacat
      541 aatctactaa attttttata tttttttgtt ttaataaatc taaaaacttt atttttttaa
      601 gaaattctgt agctaattcg ctttcttcat cacttatact ctttctcttc ttaaaattaa
      661 acagtttata tttaaacata tcctcatctg atggatctag tattttgaat tcattatctc
      721 tactcgctat tactattggt atcacactaa gaacttctgg atactctttt aataaatttt
      781 tagtttcgtt ttctatatct tcttttccta taagatagtt taagatattt aactgaacct
      841 ctatctttct agtattttcc aaagctttat tccagttaac ataataatcc cagctagcta
      901 tagtaccttt taaattttca agtaattcct taaatatttc atccttactc ttatttttat
      961 gaatattctt aaccaattcc atttgattcc ccacctcttc ataattagta attagcaatt
     1021 cagttataaa acctcttcca ttccccttag aatttatgaa tcttttagca tgtactcttt
     1081 ttatattaaa atctgaatac aaatcatcaa agaaagtatc ttcttcatct aaaacttttg
     1141 gatcagaatt acttaacatc aattttgtat ctattttatc tagaacttta taaaatttac
     1201 ttaactcaat ttgactatca tcattaaaat tacctttgtt ataggaatta aatcctccta
     1261 tagttactgg tctatatggt ggatctagat acacaaaaca atttttatct attatatttt
     1321 ctagatttaa ataattctta tttaatattt ttacttttag ctctcctcga gaatctcttt
     1381 tatttaaaag catggataat ttttcaagtt catttttatc aaaaaaacta ggatttttat
     1441 atcttccaaa aggtacatta aattcacctc gagaattttc tctatataat ccattaaaac
     1501 atgttttatt taaaaatata aaatatgaag cttgctttat acaatcttca ttttctatat
     1561 ttttttcatt tgtaagtata ttaaatctat ctctaatatc atagtacatc ttttctctat
     1621 ctgaagaacc ttcttcgtaa gatatgtatt gttcttttaa attagttagt tctgttatta
     1681 actcttccaa atcatctctg actttaatat ataaattaat taacttacta tttatatcat
     1741 taattacata cttgttgaaa atgtcctctt ctaacaaata tagaaatagt gcacctgcac
     1801 ctacaaaagg ttctatataa gtcatggaac taaaatcaca acctgtaggc atttgttttc
     1861 ttagaatatc aagtagctgt gttttacctc cagcccattt taaaattggt ttcatgcaac
     1921 acctctctca gttatattgt tatataatta tataaaataa gattctaaaa tacaagtact
     1981 catcagaaca ctatatttat atcaaatctt tccaaaatct aagtattatt ttatcctaac
     2041 acttttcaaa acctctaaat aatatacaat attctctaat atttacttac taaaaattta
     2101 aatctatctt taagtataac ctaaaacaga ttataagtta cgtagttttt aatataaaat
     2161 atacttctac aagtaaagct ttaaatatca attacttatt tcataattaa tgactatgta
     2221 ccctcaaaaa aacctcattt tctatggttg gggtacggtt ggtggatttc atcaccaaaa
     2281 taaaaaatac tagtcaataa agactagtat ttccaagtat ttcatcattc ttttttcaaa
     2341 attggtggag acgaggggag tcgaacccct gtccgcaaat cttttccttg ctgcatctac
     2401 atgcttagcc aacttcacta atatctcacc tcacatagcg ccaagtggca ggctctatgc
     2461 ttagccagtc ccaattaatc tacttcaggt cgagacaccc ctaaagtagt tccctgcata
     2521 atgacgtcca gtcttagtcg gcaggaggac taagagggac gagcagcaac taggctgcta
     2581 tcgcgaaatt atcgtctgcg tttattttaa agttccccag tttttacgtg acccagagta
     2641 acacgacatg cagcctcaag ttccaaaccc acgtcgaaac ctttacgccc ccttgagtag
     2701 tttatagtcc agtagacttt tcgcaaactt tatcaccatt tttattaaat ttttaatgta
     2761 tatataatat aatactttta agaataaaaa tcaatgttat gttctagata ataaagaatt
     2821 ttattctaaa aaatacagtt ttatctagtt taatacttac ctgtcatttc tctttctata
     2881 cgtctttgtg catctttctt agccatatct tgacgtttat catatagttt tttacctttt
     2941 gctaaggcaa tctctacttt aactttaccg ttttttagat acaaactaag tggtataagt
     3001 gaatacccct ttatagtagt tgctccaata agctttctta tttcatgctt atgaagtaat
     3061 agttttctaa ctcttaaaga atcaacattg aatatatttc cttgctcata tggacttata
     3121 tgaacttgtt ttaaaaatac ttcgctatta tctacagatg caaatccatc acttaggtta
     3181 actttgcctt gtcttattga ttttacttct gttcctttta attcaattcc acattcataa
     3241 gtttcttcta taaaatattc atgtcttgct cttttgtttg ttgctaatat ttttttacca
     3301 gccatataaa tctcaccttc tttgctagaa atttattcca taaatattag aaatttacta
     3361 aatatttaat gttgccaaat tatttgacta acttgtatga taacatattt aggcatttta
     3421 gtcaatattt cactgatata ttaagttagt taggctatat ataattaagt aatataaatg
     3481 tatgaaaata gctaaaaaac tacttcgaat atacttaata ctaaactaac tctaaccagt
     3541 aatactaatt tataaatttc aaaacaaaaa tattacatat tata
//
LOCUS       DAYASV010000228         3562 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYASV010000228 DAYASV010000000
VERSION     DAYASV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.233"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            292..807
                     /locus_tag="ACGI68_003678"
     CDS             292..807
                     /locus_tag="ACGI68_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFH0140079.1"
                     /translation="MNDARIISQIKAGKKELFDTLIKKYYDSIYCYCYRHVDNKMTAQ
                     DLTQDVFVTVINNIENYKHYGKFENYLYVIAKNKCKDFWKKKQPIYLESLDKEISNQE
                     LSDIENKMFIKEALTKLPELEFEVIILRYYHDLRFKDIAKIMESSVSVTKYRMSRALK
                     KLELYLEGGKL"
     gene            804..1565
                     /locus_tag="ACGI68_003679"
     CDS             804..1565
                     /locus_tag="ACGI68_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140080.1"
                     /translation="MNNKEIKRSLQKYKVPKYDSSALEKTIKTIHELTLSNTYKRMNN
                     KDFFISQIGFIRKRTWIMQFLILCTICIFLFSTSADSNSFYNSLSLISIASPLFVLTN
                     IEELTKVYNSSILEIEFSTKNTLHKIIATRMLIFGISDLLFMIIILVSAKQIIDVNIL
                     YIIIYILVPFNLMCIGCMEIINRNKGKHTNYYCMIYGISLVLLIVTLSNFRDNLYQFE
                     AINQWLIVFLVTAVTLIYEIRKTWRHLCSFENIII"
     gene            1603..2469
                     /locus_tag="ACGI68_003680"
     CDS             1603..2469
                     /locus_tag="ACGI68_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140081.1"
                     /translation="MELSVERLTKQYNNKIIVDKIDFKLNEGIYGFLGENGAGKTTLM
                     RMICGILSPTSGEVKVDGKNNLDMGEDFRDMIGYLPQNFGYYPDFTAKEFMLYIASLK
                     GLMPSYAKRKTQELLEMVGLSEVINKKIRTFSGGMKQRLGFAQTLLNDPKILILDEPT
                     AGLDPKERVRFRNLISEFSKEKIVILSTHIVSDVEYIADKILIMKKGRFVLTGSIEEV
                     TSKIKDKVWKCVVNENEIDYMSDRYCLANIHHSGGVANLRIVSDIKPHDNACNLKPTL
                     EDLYLYCFNEVL"
     gene            2490..>3562
                     /locus_tag="ACGI68_003681"
     CDS             2490..>3562
                     /locus_tag="ACGI68_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0140082.1"
                     /translation="MSTLIKFELKKIIKRKSTIAVILGSLFLSIILFALMPIQEKAND
                     ESGKEYRGLQAIELKKQQSKLNEGYITDKKVKNSIRDYQKMFNNPNNITTDEGGFSNF
                     KPDIYWKFFYPNKAFYNFIIESYSNPNDYNPEIIKSMPLEDGVNFYKFRNEKVNTILN
                     MEYPDKNYTQKEKDFWLSKNKDIEEPYLNGYYQGWNYFLLARDLLIFTIVAVCISTAS
                     VFSGEYQSGADSIILSSKYGKTKVITAKIVSSFLFGTIIFTINTIVSIGILLLSFGID
                     GWNLPLQLTTSSIPYPYTFLQAILLSVLIIYMILILLISFTLLLSSYMKSPFPVLITN
                     IILLFVPIFLKYSETNKLYNQIL"
BASE COUNT         1446 a          367 c          538 g         1211 t
ORIGIN      
        1 tcaattgaat atctgtcttt ttgttatata agatgttaaa agaaacttat actatgtaag
       61 taaaatttat aaatataaag ttgttcattg ttgaattaca gacacttaag atgaaaagta
      121 tgacatttga tataataaaa ttgtttaagt attaattatg agtattaatt aatggtatag
      181 ataattttaa tataactgta tttataaata attataaaat aataaattaa tttttttata
      241 ctgacttttt tatagtataa atcgttatat ataatgacgg aggtgataat agtgaatgat
      301 gctcggatta tatcacaaat aaaagcagga aaaaaagaat tgtttgatac tctaataaaa
      361 aaatactatg atagcatcta ctgttattgt tatcgccatg tagacaataa aatgactgca
      421 caagatttaa ctcaagatgt atttgtaaca gttataaata atatagaaaa ttataaacat
      481 tatggtaagt ttgaaaacta tttgtatgtt attgcaaaaa acaagtgtaa ggatttttgg
      541 aagaaaaaac aaccaatata tttagagtca ttagataagg agatatcaaa tcaagaatta
      601 tctgatatag aaaataaaat gtttataaaa gaagctttga ctaaattacc agaattagaa
      661 tttgaagtga taattttacg gtattaccat gatttaagat ttaaggatat tgctaaaatt
      721 atggagtcaa gcgtctctgt cacaaagtac aggatgagta gggcattaaa aaaactagaa
      781 ttatatctgg aaggaggtaa attatgaata ataaagaaat aaaaagaagt ctacagaagt
      841 ataaggttcc caaatatgat tcttcagctc ttgaaaagac tattaaaact atacatgagt
      901 taacgcttag taatacttat aaaagaatga ataataaaga cttttttata tctcaaattg
      961 gttttataag aaaaagaact tggattatgc aatttctaat actatgtaca atatgtatat
     1021 ttttatttag tacatcagca gatagtaatt cattttataa tagtttatcc ttgatttcta
     1081 tagcatcacc actttttgta ctgacaaata tagaagaatt aacaaaagtg tataatagta
     1141 gtattttaga gatagaattt tcaacaaaaa atacattaca taaaattatt gctactagaa
     1201 tgcttatatt tggaataagt gatttattgt ttatgattat tattttggta tctgcaaaac
     1261 aaatcattga tgtaaacatt ctgtatataa taatttatat actagttcct tttaacctaa
     1321 tgtgtatagg atgtatggaa attataaata gaaataaagg taagcataca aattattatt
     1381 gtatgatata cggtatatct cttgtattat taatagttac actgagtaac tttagagata
     1441 atctatatca atttgaagca ataaatcaat ggctaattgt ttttctagtc acagcagtaa
     1501 cattaatata tgaaataaga aaaacgtgga gacatctttg ttcatttgaa aatattatta
     1561 tctaagtaaa aaataagaat acacagtatc aggaggaata gtatggagtt aagtgtagaa
     1621 cgtttaacaa aacagtataa caataaaatt atagtagata aaattgactt taaattaaat
     1681 gagggtatat atggattttt aggagaaaat ggagctggaa aaacaactct tatgagaatg
     1741 atttgcggaa tattaagtcc tacatctgga gaagtaaagg tggatggtaa aaataatttg
     1801 gatatgggag aagattttag agatatgatt ggataccttc ctcaaaactt tggatactac
     1861 ccagatttta ctgccaaaga atttatgcta tatatagcat cattaaaagg attaatgcct
     1921 agctatgcaa agagaaaaac acaagaatta ttagaaatgg taggattatc agaggtaatt
     1981 aataaaaaaa taagaacatt ttctggaggt atgaagcaaa ggctaggatt tgctcaaaca
     2041 ctacttaatg accccaaaat tttaatatta gatgagccaa cagcagggct agaccctaaa
     2101 gaacgagtta gatttcgcaa cctaatttca gaattttcta aggaaaagat tgttatattg
     2161 tctactcata ttgtgtctga tgtagaatat attgcagata aaattttaat aatgaaaaaa
     2221 ggaagatttg ttttaactgg ctcaatagaa gaagtaactt caaagataaa agataaggta
     2281 tggaaatgtg tggtcaatga gaatgaaata gattatatgt ctgatagata ttgtttagca
     2341 aatatccatc attctggtgg agttgcaaat cttagaattg taagtgatat aaagccacat
     2401 gacaatgcat gcaatttaaa accaacatta gaagatttat atttatattg tttcaacgaa
     2461 gtactataaa tatacataag gagataggta tgtctacatt aattaaattt gagttaaaaa
     2521 aaataataaa aagaaagtct acaatagcag ttattttagg aagtttattc ttaagcatca
     2581 ttctttttgc attaatgcca attcaggaaa aagctaatga tgaaagtgga aaagagtata
     2641 ggggtttaca ggctattgag ctaaaaaagc aacaatctaa attaaatgag ggatacataa
     2701 cagataaaaa agtaaaaaac tcaatcagag attaccaaaa aatgtttaat aatccaaata
     2761 atattacaac agatgaaggt gggttttcaa attttaaacc agatatttat tggaaattct
     2821 tctatccaaa taaagctttt tataatttta taatagagtc atatagtaat ccaaatgatt
     2881 ataatccaga aataattaaa tcaatgcctt tagaagatgg agttaatttt tataaattta
     2941 gaaatgaaaa agtaaataca atattaaata tggaatatcc tgataaaaat tacacacaga
     3001 aagagaaaga tttttggttg tctaaaaata aggatataga agagccttat ttaaatggat
     3061 attatcaagg atggaattat tttctattgg ctagagattt actaattttt actatagtag
     3121 ctgtctgcat aagtacagct tctgtatttt caggagaata ccaatccggt gctgattcaa
     3181 taatattatc ttctaaatat ggaaaaacaa aagttataac agctaaaata gtaagttcct
     3241 ttttatttgg tacgattatt tttacaataa atacaattgt gtctattggt atacttcttt
     3301 tatcttttgg aattgatgga tggaatttac cattacaact tacaaccagt tcaattccat
     3361 acccatatac atttttacag gcaatattac taagtgtgtt aattatttat atgatattaa
     3421 tactattaat tagttttaca cttttattat catcttatat gaaatcacct tttccagttt
     3481 taattacaaa tataatatta ctatttgtac caatattttt aaaatatagt gaaacaaata
     3541 agttgtataa tcaaattctc aa
//
LOCUS       DAYASV010000229         3414 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYASV010000229 DAYASV010000000
VERSION     DAYASV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.234"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(169..1185)
                     /locus_tag="ACGI68_003682"
     CDS             complement(169..1185)
                     /locus_tag="ACGI68_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0140083.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            complement(1368..1730)
                     /gene="spoVAE"
                     /locus_tag="ACGI68_003683"
     CDS             complement(1368..1730)
                     /gene="spoVAE"
                     /locus_tag="ACGI68_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HFH0140084.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            complement(1744..2757)
                     /gene="spoVAD"
                     /locus_tag="ACGI68_003684"
     CDS             complement(1744..2757)
                     /gene="spoVAD"
                     /locus_tag="ACGI68_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HFH0140085.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            complement(2924..3361)
                     /gene="spoVAC"
                     /locus_tag="ACGI68_003685"
     CDS             complement(2924..3361)
                     /gene="spoVAC"
                     /locus_tag="ACGI68_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HFH0140086.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
BASE COUNT         1094 a          624 c          373 g         1323 t
ORIGIN      
        1 ataattgaat aaaattattc attcatatta taaaaactat ctcaataaat aaaggagtac
       61 aatgtactta tatacaccta ctccttttaa tttttattta tattatttgt taaaaaactt
      121 cacataatct ataatatttt taataaacgt ttgatactta aaagcttact actttattct
      181 tttaacgcta tttcgtatta ttaaccttgg atataatttt cttggaatgt gttctgtact
      241 ctttttattt attatattta ctaaactatc tactccattt tttataactt gctctcttgg
      301 gtcatctatt gttgataaat gcgacctaaa atatgatatt aattcaatat tattataccc
      361 tattatggaa atatcttctg gtacatttat accataagta gtttttaact cttgtattat
      421 agaaatcgct ggtacatctt gttgtacaaa tatagctgaa gcttttttta ttttatcaat
      481 gtatttttct aaaaattcaa cttgagattc aaaatccatt ttagatttaa tgacttctgt
      541 ctttaaattt gcttcttcca tagcattcaa atatcccttg gttctatctt catgcatttt
      601 tgaactctta tcatctgaag ttactgttat tatatcttta tgaccatggt caattaaatg
      661 cttggtagct atataacctc catacacgtt atcgtcccaa aagaaattat ctacttctcc
      721 tataaaagat ggtttaaaat atataaatac atgtggaaaa ttattgtctt ttaaaaaatc
      781 atactcttct ttttttattg attgagacac aattactaat ccgtcaacca tttgtccatt
      841 taccatttta tatacattac tatcaccact aatgttatta ttaggctgta ttataaaatc
      901 gtatttatta gtctcaatag aatttataga actatccatt atatcactaa aaaactttct
      961 agtatcttga tgattaaaat tatttgaaaa gattacccct atcctgttag tttctttttt
     1021 tgctaaattt cttgcattta cattaaaatg gaaccctagt ctatttgcct cttctaccac
     1081 tttctttttt gttttttcag atacattagg attattgttt aagcacctag aaacagttga
     1141 ataactgata cctagattcc ttgcaatatc tttaatagtt accatcttta tcccctactt
     1201 cacttcattt tattttgata atataatact acactatgtt gtaaataatt gtcaacttca
     1261 attatgtaaa cgcttcttta tataaaaaac aggtggaatc taaaagatat ccacctgctt
     1321 tataaaaagt tattatttca aaaacttatt attcttttaa taaagattta tggttttgct
     1381 tttggagtaa atactactga cataatatat ccaaagaata tagctgctgc tattccacca
     1441 gctgtagcag ttgtacctcc taaaaaaata cctaaaaaac catcactttg aattgatttt
     1501 attactcctg tagcaagtga atatccaaat cctattatag gaactgttgc acctgctgct
     1561 gcaaacttaa ctaatggctc ataaagtcct agaaaagtta aaactacacc agttgttaca
     1621 tacgttacca taacatatgg agtttgcatt ttgaaataat ccatcattac ttgggctatt
     1681 aaacatataa ttccacctac aataaatact cttacatagt ccattaacat attatatcac
     1741 ttcctactca tttactatta ctactgcatg tgcaacacat ggtattgtct gcttttgtag
     1801 tgtacttgtc ggtgaaagta gtgctcctgt tgatacaagc attactttgt taaattcttt
     1861 ttttctcaac ttatcatata tatatcctgc aaacactgta gctgaacacc cacatccact
     1921 tccaccacag tggacatctt gttctttaag gttaaacatc tctataccac agtcagtata
     1981 aacttttgat atatctacac ctttttcttt tagaaagtct tctgctatct gttttccaag
     2041 agtacctaaa tctccagtag caattatatc aaagctattt gggtcatcat tagtatcttc
     2101 aaagtaagag tagatagtat ctattgctgc tggagccata gctggtccca tattattacc
     2161 atcatcaata ccctcatcta ttactttacc aacagtaaca tactttactt ttggaccgtc
     2221 tccattagga gctaataata cacttcctgc tcctgtaact gtccactggg ctgtcattgg
     2281 tttttgattt ccaagttcaa gtgggaatct aaattgtctt tctgctgtac aatagtgact
     2341 tgatgtacct gacagtacta aatcagcaaa accaccatct ataagcatag aacctataca
     2401 aagaccttct gccattgtag aacaagctcc atatatacct aaaaatggaa tagccagttc
     2461 tcttgctgca aaggatgctg gaactatttg atttattaaa tcgccaccta gcatataatt
     2521 tacatctgac aattttttac ctgccttttg gatagcttgt tgagatgcag tctctaccat
     2581 tttactttca gctaattccc aagtcttttc accatataaa tcgtcagtca taatcatatc
     2641 aaaatagtcc tttagtggtc cttcaccttc cttaggacct actatagttc cagttgatat
     2701 tatggttggt ttattttcta acttgactgt tctttttcca attcttttat ttttcatatt
     2761 ttcacacctt tttaattttg cattttacta ttcaaatttt taatattaat taaaaatatt
     2821 aattaaatct gcagaattat ttttcaatta gttactttag tagctatttt cagattttat
     2881 accttcttca taattaataa aatttaaatt aaataaaaat catctaaaac atcttaaaaa
     2941 tataataaat aattccacat aaaatagaag aacctatccc ataaactaaa actggtcctg
     3001 caattttaaa taagtttgca cctacaccta atacatagcc ttctctttta tactccattg
     3061 ctggagatac tattgagtta gcaaaacctg ttattggaat tatagaacca gctccagctc
     3121 tttttcctat taggtcgtaa actcctaatc cagttaaaaa tgcccctata aatattaatg
     3181 ttattgatgt tgcagaacta gcacctagtt tatcaagacc tccaactttc atatacaaat
     3241 catttattgc ttgccctatc atacaaataa ttccacctac aataaatgca agtgtatagt
     3301 tttttaaata tgtaggtttt gggcttattt ggtctacata ttttttataa tttttatcca
     3361 tactaaacac ctctctaaag tattatttgt taattttaaa aaaataaacc tatt
//
LOCUS       DAYASV010000230         3365 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYASV010000230 DAYASV010000000
VERSION     DAYASV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.235"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            247..1515
                     /gene="eam"
                     /locus_tag="ACGI68_003686"
     CDS             247..1515
                     /gene="eam"
                     /locus_tag="ACGI68_003686"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016869 - intramolecular aminotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006536 - glutamate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HFH0140087.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDTCDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1608..1964)
                     /locus_tag="ACGI68_003687"
     CDS             complement(1608..1964)
                     /locus_tag="ACGI68_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140088.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2115..3209)
                     /locus_tag="ACGI68_003688"
     CDS             complement(2115..3209)
                     /locus_tag="ACGI68_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0140089.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         1377 a          442 c          478 g         1068 t
ORIGIN      
        1 tcttaaaaat agaaaaaatt aatcatttgt taaaaaaaat gcataattta ttgtaattta
       61 ttgacgaagg ttgaaaaata ttcataaatg tgcgaaataa cagaaaaaat gcaaaaatta
      121 attcaatttc atttgttata aaatctatat ggcaaaatta aatctgccta tcagaatgat
      181 ataaacacac cagaaaggag gacgctatca aataatacaa gtagttaact ttgatagcaa
      241 aaaattatga atgaacaaac tagaatatcc ttagagagag ctgctgaatt aaaatcaaaa
      301 attgatgatt atattcaggc tagaaaaacg attaacagag gtcttgaaaa agaagaagag
      361 ataaataaac gaaaacagaa aatattaagt atcttaaatg gaactgaaga ggattggaat
      421 aactacaaat ggcaattatc aaatagaata acagatgtag atactttatc aaaaattata
      481 actctaacta aaaaagaaaa agaatatata aaagaggttg gtactcaatt tagatgggca
      541 atatctccat attatttgag tcttatagac ccagaagata catgtgaccc aataaaatta
      601 ctgtctatac caacacatat agagttggaa gatgaacaag aagatttgga cccaatggga
      661 gaagagtata caaacccagc aggatgtata actagaagat acccggatag attaataata
      721 aatgtaacaa atgagtgtgc tatgtattgt agacactgtc agagaagaag aaatattgga
      781 caacaagatt ctcataagtc aaaagctatt atccaagaat ctatagacta tatcagagaa
      841 aatgaagaaa taagagatgt actagtaact ggtggagatg ctcttacatt aaaagatgat
      901 tatttagagt ggattcttag ccaacttaaa gagataccac atgttgatta tgttagatta
      961 ggtactagga ctcttgttac aatgccacaa agaatcacag atgaattttg caatatgcta
     1021 aaaaaatatc accctgtata tataaatact cattttaatc atccaatgga aataactaag
     1081 gaatctaaag aagcttgtga aaagttagca aatgcaggag ttccattagg aaatcaggca
     1141 gtattattaa atggaataaa taatgataaa tttgtaatga gatgtttaaa tcaagaatta
     1201 ctgaaaataa gagtaaaacc ttattatata ttccaaagta aacatgtaaa gggaacaaaa
     1261 catttcaata catcagtaga tgatggtctt gaaatcatgg agtatttaag aggatataca
     1321 tcaggaatgg ctataccaac atatatagta aatgctccaa aaggaggcgg aaagactcct
     1381 ttgcttcctc aataccttgt atcaaaagga acagattacg ttatgcttag aacatgggaa
     1441 ggaaaagtta taaaaatgga agatgaacct gctgtagata taaagaaact tataaaagaa
     1501 caagcgcagg attaaaaaag tatttaaagt ttataaaaag aaacaacttt aaaataaaaa
     1561 gaaaatggat gtcaaattta tatcaatttg catccatttt ttattagtta tttattattt
     1621 tgttgttgat taactaattc aagatatcca cttgggtgga agtaatatat ctcagaagta
     1681 ttttcattta caaagtaacc aatatcacca tctgcatctg gtaaaaatac ataaccatta
     1741 agatttttag cttttaatgc ttcaaaatca ctagcatttc cttgatatat gtaatcaact
     1801 tcattgttgt attttactaa aagttcttta gcttgttctt gagtcaattt agtattttgg
     1861 cttacaggaa cattcttttg agaatctgca taagaagatt catttactgg tgcagttaat
     1921 aacaaacaag ataaaactaa taaagatata agtgcttttt tcatactaaa accctctttc
     1981 atgtttaatt atataagatt cattacttaa taatattgta tgtttcctat aattaaatat
     2041 taccattaaa gagaataata ttacagttac acagctgtaa cttttgatta caaaatcgta
     2101 attaaattgt atttttattc catttgtatt ttatcatgtc tacgatttac aaactcacag
     2161 aagtatatct ttataacgcc catcacagga gcagatagaa tcataccagg tataccaaag
     2221 aatcctccac caacagtaac tgctaggatt gtaaagaatg ggctaagacc tagttgtcca
     2281 cctacgattt ttggctctat aaatgtaact tcaacttgtt gaattattat taagtaaatt
     2341 aaagaagtta aagcaattgt tggattggag aataaattaa ttattacaac tggtgccata
     2401 cctattactg gaccaaaata tggaaccata ttcatgacac ccataagtgt accaaataat
     2461 agtgcatatt cagattttaa aaagtaaaga ccaattccag aaagtactcc aactataaaa
     2521 gaatctaaaa tcttacctgt aaagtattta ccaatattta aatttaagct ttgacatagt
     2581 tctattataa agtctccata ttttttacct aaggaaatat aaactacttt ttttgtaaac
     2641 agtaagaatt tttctttttc taataatata tagaaacaaa ttataaaacc taaaagaaat
     2701 tgtactataa attttccaac tgaaaatgta gtgctaaata tcccaccaag tgaaccaata
     2761 agcaagttac taaatttagg cattacactc ataattttgt caccatactc ttttaatgta
     2821 ttagggtcaa ctgattgtaa actcttgcct aagtcgaata aaaactgctc tgttttatct
     2881 atgtatgtag gaatttgagc aactaaatca gctaaactat ttactatgga tggaactgta
     2941 aataatataa acgaaattag tataactatg agtacaccat aagtaagaag taacgaatat
     3001 attctcttaa aatttaattt actttctaag aactttacta gtgggttgaa tatatatgct
     3061 aatacaaatg ctattataaa tggtgttagt agtgacatta ataaacttat cactccaaaa
     3121 aaatatttgt aattatcaat aactttaatc aagatatatg aaacaactac agtgagtaca
     3181 atatttagat aaattttttt atcttgcaaa ataacacaac ctttcttata tttattaaat
     3241 ttagatatac ttaaatctaa gttaaagtta atatttatag cttattatac aataaaaagc
     3301 agtattaata tagtatgatt aatatgaaat tataaatacg ttaaaacttt atgtttatag
     3361 ctaaa
//
LOCUS       DAYASV010000231         3359 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYASV010000231 DAYASV010000000
VERSION     DAYASV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.236"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            567..3209
                     /gene="adhE"
                     /locus_tag="ACGI68_003689"
                     /gene_synonym="adhC"
     CDS             567..3209
                     /gene="adhE"
                     /locus_tag="ACGI68_003689"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFH0140090.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT         1312 a          418 c          628 g         1001 t
ORIGIN      
        1 ttatcattaa aaaattaacg gtcttaatat aaaaattatt tatctatatg aaaagcataa
       61 ttattacata ttgacttaaa aataacttat agagattaga ataattttag tttatatatt
      121 ttttaaaact atacagttgt tataaaaaga aaaattgcat acaagtcaaa aataagtgtt
      181 attttataaa aatacgacaa aaattttatt tactacttat acaaataaca tgcaaataac
      241 atataaaatt taataagtaa aaaaaatgaa agatataaat tatcatttta gtattttatt
      301 tagtcgaagt aaattattca aatgaaatat atctcattta gataaaaaat tttagtaaaa
      361 gtaaaaaaaa taatatattt gttaaaaaat aaattgacaa aatattcaga aaaaaacttc
      421 ttgacctaag agagtaaaat aaataaaatc taaggtatag agatgaaatc tataaaatta
      481 ttttaagtat aagttaaaaa tatagatgat gcatttaaga tttataaaaa ttagagatgc
      541 ataattattt tagggaggtt tttagtatgg agaaaaaaga aaaggtagta gaaaaatcaa
      601 atgttgaagt atgtagtcct gaaattgtca acagtgttga aacattaaga atgagattag
      661 aagaaattag attggcacaa aaagaatttg caacatttac gcaagaacag gtagataaga
      721 ttttcttagc tgcttcaact gcagctaatc aacaaagaat acctctagca aaaatggcag
      781 tggaagagac tggtatgggt attgttgaag ataaggttat aaaaaaccat tttgcctcag
      841 aatatatcta taatgcttat aaagatacta aaacttgtgg tgtcatagaa aaagatgaag
      901 catttggttt tacaagaatt gctgagccag taggtgtttt atcagcagtt ataccaacaa
      961 ctaatcctac atcaactgca atattcaaat ccctaatagc actaaaaact agaaatggta
     1021 taattttctc tcctcatcca agagctaaaa actgtactat agaagcagct agagttgtac
     1081 atgatgcagc agttaaggca ggagcaccaa aaggacttat aggttgggta gatgttcctt
     1141 cgattgaatt aactaatgtt gtcatggcag aggcagacct tatattagct actggtggac
     1201 ctggaatggt taaatctgca tactcttcag gtaaaccagc tgtcggagtt gggcctggaa
     1261 atgtacctgc aataatagat gagagtgcag atataaaaat ggctgtaagt tcaatcttag
     1321 tatcaaaatc attcgataat ggtatgatat gtgcttccga gcaagctgta atagtaccag
     1381 aaaaaatata tgaagaagtt aaaaaagaat ttaaatatcg tggagcacat ttcctaaata
     1441 aagaagaaac tgaaaaagta ggaaaagtag taataattga tggttcatta aatgccagaa
     1501 tagttggaca accagcacat gtaatagcca aaatggcgga tgtagaagtt ccaaaaacag
     1561 ctagaataat aattggtgaa gtagaatctg ttgaattaaa tgaacctttt gcacatgaaa
     1621 aactttctcc tgtacttgca atgtacaagt ctaaaagttt tgaagatgca gttgctaaag
     1681 cagaaaaatt agttgcagat ggaggctatg gtcatacatc ttcattatat gcagactcta
     1741 taaatcatcc agagagagta gaaaagtttg taaatgctat gaagacttgt agagtattag
     1801 ttaatactcc atcatcacaa ggtggtatag gtgatttata taactttaaa ttggcaccgt
     1861 ctcttactct tggttgtggt tcttggggtg gaaactcagt atctgaaaac gttggagtaa
     1921 aacatttgct taatatcaag acagtagctg aaaggagaga gaatatgctt tggttcagag
     1981 caccagaaaa ggtttatttc aaaaaaggat gtttaggtgt agcagcaaga gaatttaagg
     2041 atgttatgga taaaaagaaa gcatttatag taacagattc tttcctatat aataatggat
     2101 atactaagaa attaactgat ttattagatg agatgggaat taagcatact acattctttg
     2161 atgtagctcc agacccaaca ttagcttgtg caagagaagg agctaaagct atggctgatt
     2221 tccaaccaga tttaataata gctgtaggtg gaggaagtgc aatggatgct ggtaagataa
     2281 tgtgggtaat gtatgagcat ccagaagtag atttccaaga cttagctatg agatttatgg
     2341 atataagaaa aagagtatat gtatttccta agatgggtga aaaagcgtat tttgctgcta
     2401 tcccaacttc agcaggaact ggttcagaag taactccatt tgcagttata actgaccaag
     2461 attcaggtgt aaagtaccca ttagcagatt atgaattaat gccaaacatg gctataattg
     2521 atgctgatat gatgatggag atgccaccaa gattaacagc agcatcaggt gttgatgctt
     2581 taacacatgc attagaagca tatgtatcta tgttaagaac tgaaccagct gacggtctag
     2641 cactacaagc aggtaaaata atatttgaat atttacctcg tgcatataaa aatggaaaga
     2701 atgataaaga agcaagagaa aaaatggcaa tggcatctac tatggcagga atgtcttttg
     2761 caaatgcctt cttaggaata tgtcactctt tagcacataa attaggtgca ttccaccatg
     2821 ttcaacacgg tgttgcaaat gcattattaa taaatgaagt aatcaagttc aattgtgcag
     2881 aagctccaaa taagatgggt gcattctcac aatacagata tcctgattgc atacaaagat
     2941 atgctgaatt tgcttctttt gcaggtatta aaggaagtac agaccaagaa aaagtagata
     3001 atcttataaa agcaatagat gaactaaagg caaaagtagg tcttcctaag actataaaag
     3061 aggctggagt agaagaatct aaattcttag aaagattgga tgctatggtt gaacaagctt
     3121 ttgatgacca atgtacaggt gctaatccta gatacccatt gatgagtgaa ttaaaagaga
     3181 tatatctaaa agtttattat ggtaaataat atctattgtg caacataggt tctattgggt
     3241 ttgtatataa attattagat ttgaatataa atagagtttt attagagaca cgtagaagta
     3301 actataaata atagattata atttaaattc taaatggaaa tgataaaaag tcagtaatt
//
LOCUS       DAYASV010000232         3346 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYASV010000232 DAYASV010000000
VERSION     DAYASV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.237"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(280..537)
                     /locus_tag="ACGI68_003690"
     CDS             complement(280..537)
                     /locus_tag="ACGI68_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFH0140091.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            1117..1338
                     /locus_tag="ACGI68_003691"
     CDS             1117..1338
                     /locus_tag="ACGI68_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HFH0140092.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2197..2427
                     /locus_tag="ACGI68_003692"
     CDS             2197..2427
                     /locus_tag="ACGI68_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HFH0140093.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2440..2589
                     /locus_tag="ACGI68_003693"
     CDS             2440..2589
                     /locus_tag="ACGI68_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HFH0140094.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2846..3283
                     /locus_tag="ACGI68_003694"
     CDS             2846..3283
                     /locus_tag="ACGI68_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HFH0140095.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSIPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1357 a          428 c          360 g         1201 t
ORIGIN      
        1 tatttatata gtttatttat ttatttttcc cctttataca agcaattgta tcattttatc
       61 tattttttag ccaacaaata ttttcgacca ttttctttgt attctcaata tatctccagt
      121 atttaacaaa tttttttata gaatatacaa tgtatttaaa ttaataagta gtaaatttag
      181 ttaatttgtc taaaaaaact ttataaaaca tcttctttgt ctttactctt acgtttaaca
      241 aggctatttt cattatgttt aaacattatt ttattatatt taaacactat tttcattgag
      301 tattcgctta atattgctta cacacatatt atatctaata gccaattcat tgtggttgac
      361 tccattaaat tgttttacta tatccctatt tctagcatat ctaaaaagcc ccttatacgt
      421 aggtatataa gtatttgttc ctccatacaa cttggcaacc tctatgaact tatcaattcc
      481 gattatttct accattgcat gaaattgaac tggtatatcc tccactttta tcttcataag
      541 ttacacctcc tttaagatat ttttatagac ttaacaaagt ttttatttaa tacattattg
      601 actaaactat atttattagt tttattgaat ctaataaaaa atttcttagc tttaaaatta
      661 atttttgtat taacttcaat aaaatttctc ataacttata actttgttag ctttaataaa
      721 gattttaatg gtttaaaagt aaaatgttaa tattggactt ttaacttttt tatttacaga
      781 ttaaatttaa taaaattttt aacataagct ttaacattta atttaacata aactttagta
      841 cctaacattt aactctacac atatgaaaat ccatacaaaa ctatctcaac taagcatgaa
      901 tatatacaaa tactatttca aaacactaag aacatatctt cacaaataac ttgtaaactt
      961 tttcatcagc aaaaaagtaa aatttttcct ataaaaattt acacaaaaaa tctttagaaa
     1021 ttactgttta actccgatat tatcttagat gatagtatta tagctaacaa agaaagtcga
     1081 caaatctttg aaattaaatt tttaggaggt aattttatgg aagttgtttc aacaaaaaat
     1141 ttatctagta taaaactaaa attggatgct ggttttgatg ataagggtaa ggcagttgtt
     1201 aaaagtaaat catttgcaaa tgtaaaagca gaggcattaa atgaagatgt ttatgctgtt
     1261 gcagaagcaa tagcaagtct tcaagagaac cctattgtag atattttaaa attagatagt
     1321 acatcactat ctaaatagtg tcttttaaac gtactctgct ttacaaatta aaaaattaac
     1381 tctttatata aaagattaag ttatataatg cacttaatac gctatagatt tggcagatta
     1441 taacttcaat tttggttata actttaatat attaacgctt tgaaaaatta atatattaaa
     1501 cgtatttcaa cttaaacatg ttagctattt tagatgctac cctactttaa tctaattaat
     1561 tgaaataaat taaatatttt ctatatcagc aataactaga gtcaacaatt aaaatactaa
     1621 tattgacaat tgaaacactg atactaacaa gcaaaacact aatattgaca attgaaatac
     1681 taatactaac aagcaaaaca ttaatattga caattgaaat actaatacta acaagcaaaa
     1741 cactaatatt gacaattgaa atactaatac taacaagcaa aacactaata ttgacaattg
     1801 aaacactgat actaacaaac aaaacactaa tattgacaat tgaaatacta atactaacaa
     1861 gcaaaacact aatattgaca attgaaatat taatactaac aagcaagaca ctaatattga
     1921 caattgaaat attaatacta acaagcaaaa cactaatatt gacaattgaa atattaatac
     1981 taacaagcaa aacactaata ttgacaattg aaacactgat actaacaagc aagacactaa
     2041 tattgacaat tgaaatatta atactaacaa gcaaaacatt aatattgaca attgaaatat
     2101 taatactaac aagcaaaaca ttaatattga taattaaaat accaatatta acaagcaaac
     2161 aattaaaata taaattatta tatcggaggt aattatatgg aaattaagaa aaaattagtc
     2221 atgagcttta aaaatgtaaa ggataaacaa gtttcttttt ctatagaaaa tccaaaggat
     2281 gatttaactg aagaaaatat aaaatctgtc atggatttga tagtatctaa aaatattttt
     2341 tcggttggag gatttgacct agcttctgtt gttgaagcca aggttgtgga aacaaatacc
     2401 actccttatg acttagttat agaatagggg gctaatttta tggatgatag tttacaactg
     2461 ttaataacca atgttggttt tcctatagct ctatccatgt acttgttggt tagaattgag
     2521 ggtaaattaa atactttatc aaacagtatt gatacattat ccaaaaatat tttgatattt
     2581 aagaagtaat ttgacaatat atgtaagtca taaaataggt ctatttgtaa aataattaga
     2641 catattctat gtgaaatact aggatatttg gataatttat ccaaatatcc atagtatttt
     2701 tttgaatttt accacctcaa atttgaattt cactgtttta tttttgaatt tcactatgct
     2761 taatttgaaa tttagaacgc atctagttgg actcaataag atataatgct attctaatga
     2821 cagtaataaa atgaggtgat gattaatgaa aatacatgtc gaacaaggta ttaattgtga
     2881 tgatattgaa ataaccataa aatgttctgc tctagataat aacattgaaa aaataatttc
     2941 attattgagt ataccgttac ttgatataaa aggtaaaaaa tcaggagaga tttataaact
     3001 caatgtctgt gatattttat actttgagac agttggaaat aaaacatttg cttattatga
     3061 agatgatgta tttgaaattg atttaaaaat atataaatta tcaaaattat tagctaatac
     3121 atctttctca cgaatatcta aatccatgat tttaaatatg gactacataa aaagtataaa
     3181 agtaatatca cataatcgca tgcaagctac tttaaaaaac aacgaaaaag ttattataaa
     3241 tcgtcgttat ataaaaaata ttaaagaaaa gctaaacatt taataaaaat ctaaagtaat
     3301 ttattaatat cactaattgc aaatttattg acctatttaa taatta
//
LOCUS       DAYASV010000233         3344 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYASV010000233 DAYASV010000000
VERSION     DAYASV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.238"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(1619..1858)
                     /locus_tag="ACGI68_003695"
     CDS             complement(1619..1858)
                     /locus_tag="ACGI68_003695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140096.1"
                     /translation="MECYLKRTLIIFSEVFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVLARRRTIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            2070..3341
                     /locus_tag="ACGI68_003696"
     CDS             2070..3341
                     /locus_tag="ACGI68_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFH0140097.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIMLEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1286 a          407 c          392 g         1259 t
ORIGIN      
        1 gtacaaaata ctcctttttc ttttttacta ttgtgataat tatttagtta gtattaaaat
       61 attatcactt aaaacaaata caaaacgtct cttatatgta catttttcat tgttgtaata
      121 atgatttgtg tatgtatttc tcacaattgg agtgcttatg cactcttttt aaataccttt
      181 attaccataa cctaaaagct attttattat cccttttagt ttatttttaa gtagttaaat
      241 aaattatttt atattttttt cttcatttat atgaaacttt tgttttatca ctatatgcac
      301 tttatattta ctacaaataa cacataagct tacttttatc ataagactcc tatatctaaa
      361 tataatttaa gaggttaaat aattgaatag tatattaaaa cataatggta atgacaaaaa
      421 tataaaaatc tttatagtca taaattttat cttatcattt ttatcctctc tattaaaata
      481 attatcttga taaaatgtag ctctattttg ataacctgtt tttaaaactc cattttgaat
      541 taacagcaat ataaaaaaga ttgctaataa aataaagtta taattttttt cttttaatct
      601 aaaattagct ataataacaa tgaaactata taaagcatat aaaaattttt tcataaaaaa
      661 cacctaaatt ctatatttgt attttatatt gagccaaatc taatttctgt atatttatta
      721 agtgtagttc tagttcctat tgtagtagca ttaaatattt tttaattttt attttcaaag
      781 ggcatctcct tatatttaaa ttatagtatg aatattcata aatttcaatc tatttataag
      841 gtaattattt tttatatcta atatcttgtc attaagtttt attatatata tttataaatt
      901 tttcaatact ttttataatt attctacttt tatattttaa tcattaaaac taaagtcaat
      961 gttatctggt aattatttac tacaaatgat aaaaaataat tatattttaa aggataaaat
     1021 gctgtataaa ttattggggt attttaccta tacttgttac aaatttacat accacttagt
     1081 taatataaaa caaatttcaa agagattgga caacgaataa aaattgcaat ttatatacca
     1141 tatagttaat ataaagcata tttgtgtcga tatatagttc taactaagta taagatttac
     1201 atacctcata gttaatataa aacacatatt ttacatgttt gaagatttta tcagaaaccc
     1261 tatttacata ccgcatagtt aatataaaac tattaatagt gctatgttgt ttatttgcat
     1321 taacaagatt tacataccac atagttaata taaaacatgt ttagcaggag ttatgatgat
     1381 aaaacacatt atttatttac ataccactta gttaatataa aaccccaaaa taaactaagt
     1441 attttcagta cttacacaca tacaactctc tcaaatttgc agtgaaccat gagtagtgca
     1501 attgataaca tttatcatac accctcaatc ccttacattg caactgttaa accctacttt
     1561 atcaaaaata tcgctcactg caaaatttat atatttttat tatatcataa aattcaaact
     1621 ataaaattaa aatggaataa tttatcaatt taagaacacc tacatatttg taaatactta
     1681 ctatattatc tattctaata gttcttcttc tagctagaac taatataaaa atcatctctg
     1741 aaagattagg acatactaat ataaaaataa ctatgaatag atattttctt gttttaaacg
     1801 atatggataa aaaaacttct gaaaatataa tcaaagttct ctttaaataa cattccattt
     1861 tatgtaagta ggcattatat ttttcttaaa tgtcacttaa atgtcagtta tatataataa
     1921 tagtaaacaa ctgtatttca ctaatatttg ctattatcta atagtattta tagtttagat
     1981 ttgatataat acatgtggta aaattttgat ttgtaaaaca aaattttacc tacatatatt
     2041 aaaacaaatt taaaactaag gagacctata tgttaaaagt actaaaagga aatataattt
     2101 ttacaaaaac ttcagaaact ttcacagtat ttgaaaatag ttatattatg ctggaaaaag
     2161 gtaaagtaaa gggaatattt aaagagatac ctaaggaata caaaaaccta aaagtagtag
     2221 attacactga taagcttata atacctggta tgaatgacct ccatgcccat gcctctcaat
     2281 ttaaaaatct tggaatggct atggataaag agcttttgcc atggcttgaa acttatacat
     2341 ttccagaaga atcaaattat aaagatatag aatatgcaac aaaaatgtat aaaaaatttg
     2401 taaaagaact ttggaaatct ggaactacaa gaatagcagt gtttgcaaca gtacataaag
     2461 aagcctctat gaaacttatg gatatattca aagaagttgg tcttggagca tacgttggta
     2521 aagtaaatat gaatatgaat tgccctgata ctttattaga aaacactcag gattctatta
     2581 ctgatacaga agaaattata aataagtata gtgatacaga ttctattgta aaaccaataa
     2641 taacacctag atttatacca agctgtacaa gtgaactttt aaaaggttta ggagatttat
     2701 gtttaaaata taatattcct attcaatcac acttatctga aaattatgat gagattgaat
     2761 gggttagaag tctagaacct gaatctaaat tctatggaga tgcatataat agatataatt
     2821 tatttggtca aactcctact ttaatggctc attgtgttca ttgttctcag gatgaaatag
     2881 atttaatgag agaaaataat gttatggcag ttcactgtcc agcttctaac tttaatgttg
     2941 gtagtggtgc tatgcctatc agaaaattaa tcgataataa tattagatta gcacttggat
     3001 cagatataag tggtggacat acattgtcaa tatttaaagc aatggtatct gcaatacaat
     3061 tatctaaatt atattgggtt aactctggta agaagtataa tttcttatct ttatctgagg
     3121 ctttttatat tgcaaccaaa agtggtggaa gtttctttgg aaaagttggt agctttgaag
     3181 aaggttatga ttttgatgca ttgattattg atgattcaaa tttaaatcat gacaattatt
     3241 ctatacttga aagacttgaa agatttatat atgttggtga tgatagaaat atcattcata
     3301 gatatgtatg tggaaaacta attgaagaac ctaatatata atta
//
LOCUS       DAYASV010000234         3074 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYASV010000234 DAYASV010000000
VERSION     DAYASV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.239"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            225..491
                     /gene="rpsT"
                     /locus_tag="ACGI68_003697"
     CDS             225..491
                     /gene="rpsT"
                     /locus_tag="ACGI68_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFH0140098.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            complement(678..1721)
                     /gene="holA"
                     /locus_tag="ACGI68_003698"
     CDS             complement(678..1721)
                     /gene="holA"
                     /locus_tag="ACGI68_003698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFH0140099.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(1675..2976)
                     /locus_tag="ACGI68_003699"
     CDS             complement(1675..2976)
                     /locus_tag="ACGI68_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HFH0140100.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
BASE COUNT         1136 a          441 c          283 g         1214 t
ORIGIN      
        1 ataaaatatt aatttatata tgtcaaaata tttaaaacac aaaatatacc aatttaaaaa
       61 caagatattg caaattattt atacacaatt tacagtctaa aaaatcaaaa taaaaaacat
      121 tattaaaata tataaataat aatccttgat ttttagattt atttctgtta aaatctaata
      181 tgttgacgat gaatatgata aaaaaataca gggaggtgaa cacggtggca aatataaaat
      241 cagctaagaa aagaataagt gttatcgaaa agaaaactgc tttaaataga gttagaaaat
      301 ctcaaataaa aactgcaata agaagattcg aagatgctgt tgcagctgga aacagagagg
      361 atgctgttgc taagttccaa tacgctcaaa aaagaatata ccaagtagct tctaaaggaa
      421 ctatacataa gaatgctgca gctagaaaag tagctaaatt agctcaaaaa ttaaacggta
      481 tgaacgctta attaccagtt tatatttctt atatataata acccctatgt aggggttttt
      541 tattttgtaa atttatacaa taaaaaataa atagttagac atattatagt tgattgtata
      601 tcaaacttaa tttagcttac atctactctg aactataaca tatcttgcta tttatttttt
      661 attaatatat agatttttta aattgcttct cttttacagt atctactaac caacatttca
      721 actgctaaag tatctcttat aagacctgtc tttattttat aatcactttc taatatatag
      781 tttaatattt ccacaattat atcatcagaa aaatttttag tttgctttaa tgcttttcct
      841 acaacaaatt gatgcatttt taatttatca gctataagtt ttgttgaata tccatcaagt
      901 tgtaactgtc ttacttgcat aattatttta aattgtcttg ctatcataga aaatatacct
      961 aatacagatt cgccttcttg tatcatatca tttaatattt tcattgcatt agaagcattt
     1021 tgttctccaa tataatcgat taatttgaat atatcattct caactttttt ttgagataat
     1081 ttatcaatta catcattggt tacattattc cctttaccaa caaatgaact tattttattt
     1141 atttcatttt ctaaatcaga taatgtcttt tcactactct tatctctgta tccttcttgt
     1201 tcaataaaat acatgatttg agaattatct attataacat catttaaagc aaaagatttt
     1261 tttatccatt taaacaaatc catatcagaa agcttatcac aatcaaaaac aattccattg
     1321 tttcctattt tcttaactaa tgattttctt ttgtctacat ctccatatac aacaaaaact
     1381 atagttgtgg tgtctggaat attgtctaaa tgctctatta agtatttctc atcactatct
     1441 gtaaaattct ttttctttcc ttttaatagt tcaaagtctt ttattattac tatctttcta
     1501 tcatccataa atggaagtgt ttctatagag cttataagct gatttaagac tatttccttc
     1561 ccatctatta tatctaagtt aaaatcaagc attccctcgt ttagactatc tttaaatgct
     1621 tttattgcat tctcaattaa ataatactct cttccataaa aaagatacat tttttcaaag
     1681 tttttctctt ttacattcct tataatatcc ttgtaattca ttctcttgct cctttatctc
     1741 ttttccttct atgtaaaata tgtaaaaaaa cactcctatg taataaataa caacaatata
     1801 aaatttagga ttttctatat taatgtatgc aaaacttaaa ttgctgattt tttctagtaa
     1861 aaaaaatatg ctttctataa atgtactatt aaaaaaagat ataattttag ctattactac
     1921 acttacttta aatacgatta aacttgcaat acttaaatac attattactc ccacaaatgg
     1981 tactataatt aaatttccta taattgaaag caatggtata cctttaaatg tataataaat
     2041 tataggtaaa gttaatatat ttgatgatat tgtcaatgat accatactta attttataat
     2101 actatttatt ttattataga atattaatat agaaagtgta gctaaaaaag acaattgaaa
     2161 acttacatta tatataacat aggggttatt cataattaaa attatcccaa ttagagacaa
     2221 tgccgatatt ccatctttct ttctatcaat aaatattgat aaataaaaaa tcataacaaa
     2281 tgctatagac ctaattatgg atggagacgc acctaccatt atacaatata aagttataaa
     2341 aatcgacagt attaataatt tatacaattt atttatacct cttaatagaa aggatataat
     2401 tacacataat attcctgtat gaagacctga tatagatatt acatgactag ttccagttct
     2461 agtaaaaatt tctttttgtt cctcagtcaa attttcaact tctgctaata gtatagaatt
     2521 tataaaatct gagtttttct tatataagta cctataagtt ttactaatat aaaactttat
     2581 ttcccctatt aaagagtaaa gtttattttt atcaataatt ttatagttat ttatatacac
     2641 tataccttta tatccagtac tttttatata ccttccataa tcaaaatcat catacttcat
     2701 tttatctaaa cttttgaatt tacctgtaat attaactttc tgaccaatct tcaaattttt
     2761 attttttgta taatcattta ttagaaaaac ccctaccttg tactgattgt atcttttctt
     2821 ttcaattttt cctttgacaa ctccttgtat agttattatc ttatcattaa gttcttcgtc
     2881 gttttttgtg ttatttgtaa tgataaatga tatagctacc acaaacatta atataattaa
     2941 taatggtctt cttatttgaa ttataatcac tcccatataa aagtatatta tttagattat
     3001 tttcaaatag aaaattttta aacaagataa attgttatct aaacaaaaaa atacgtcaaa
     3061 agatttttaa aatc
//
LOCUS       DAYASV010000235         3044 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYASV010000235 DAYASV010000000
VERSION     DAYASV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.240"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            102..1148
                     /gene="ilvC"
                     /locus_tag="ACGI68_003700"
     CDS             102..1148
                     /gene="ilvC"
                     /locus_tag="ACGI68_003700"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFH0140101.1"
                     /translation="MSNKNKYHKNKKKYWEEMKMARMYYEKDVDLEVLKNKKVAVLGY
                     GSQGHAHAQNLRDNGVHVVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPD
                     EKQKKVYEESVKDNLKEGQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVF
                     TKGSGVPALFAVYQDYTKKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVL
                     CGGISELIKLGYKTLVDAGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEY
                     GDYVSGKRVITDVAKQGMQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIA
                     EVGRNLRSMMSFLK"
     gene            1201..2889
                     /gene="ilvB"
                     /locus_tag="ACGI68_003701"
     CDS             1201..2889
                     /gene="ilvB"
                     /locus_tag="ACGI68_003701"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFH0140102.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
BASE COUNT         1205 a          312 c          611 g          916 t
ORIGIN      
        1 agctaataaa aactgaaaat tctaaaatta taattgtaag attatataaa tttagaattt
       61 aaatctgttg aataatatta aaacaatgat aatgagtatg aatttctaat aaaaataaat
      121 atcataaaaa taagaaaaag tattgggagg aaatgaaaat ggcaagaatg tattatgaaa
      181 aagatgtgga tttagaagtt ttaaaaaata aaaaggttgc agttttaggt tatggaagtc
      241 aaggacatgc acatgcacaa aatcttagag ataacggtgt acatgtagtg ataggtcttt
      301 atgacggaag taaatcagca caaaaagcaa aagaagatgg atttgaagtt aagagtgtag
      361 cagaagctac aaaagaaagt gatttgacaa tgatgttaat gcctgatgaa aaacaaaaga
      421 aagtttatga agagagtgta aaggataact taaaagaagg ccagacatta gcttttgctc
      481 atggattcaa tatacattat aatcaagtac aaccaccaga atttgttgat gttgtaatgg
      541 tagctcctaa aggacctggt cacttagtta gaaatgtatt tacaaaggga agtggagttc
      601 ctgcactatt tgcagtatat caagactaca ctaaaaaagc tacagagaca gttttagcat
      661 atgcaaaagg gataggagca acaagagcag gtgttttaga aactactttt aaagaagaaa
      721 cagaaacaga tttatttgga gaacaatcag tactttgtgg tggaataagt gaacttataa
      781 aattgggata taaaacgctt gtagatgcag gttatcaaaa agaagtagca tattttgaat
      841 gtttacatga aatgaaacta atagtcgacc ttatatatga aggtggattt gaaagaatga
      901 gatatagtat aagtgacaca gcagaatatg gagattatgt atctggtaaa agggtaataa
      961 ctgatgttgc aaaacaaggt atgcaaaatg ttttagagga tatacaaaat ggaaaatttg
     1021 caaaagcgtg gattaaagaa aatgaagaag gacgtgaaaa cttcctaaaa actagagaag
     1081 aagaatataa tacagaaatt gccgaagttg gtagaaatct aagaagtatg atgtcattct
     1141 taaaataaat atatattaaa tggattagtt tgagggaaag agtggtgtat agtagtgcgt
     1201 atgaatggag caaaggtaat tttagaatgt ttaaagaaag agggaattga cacaatattt
     1261 ggatatcctg gtggagccgt gatacctctt tatgatgctc tatacgatta ttcagatgat
     1321 tttaaacata taaggacatc ccatgaacaa ggtttagttc atgctgcaga tgggtacgca
     1381 agaagtacta atacagtagg agtttgtttt acaacatctg gtccaggagc aacaaatgcc
     1441 ataactggaa tagcaacagc ttttatggat tcttcaccta tggttgtaat ttcagggcag
     1501 gtgccaacta gtttattagg aaaagattct tttcaagaaa ttgacataac tggagcaaca
     1561 ttatctatga caaaacataa ttatttagtt agaaacacta aagaattagt tcctacaata
     1621 aaagaagctt ttagagttgc aaattcaggt agaaaaggac ctgtacttgt agatgttcca
     1681 aaagatttat ttttagcaga aatggatttt agtggtgagg actatgattt atgtcaaata
     1741 gatgattata tggattataa gagtgatttt gatctagatg atgaaactaa tattaagctt
     1801 ttaaatgaag caatagatat cataaaagaa tctaaaaagc ctataatata tgcaggtgga
     1861 ggtgttaaat cttcagacag tgaggaaatt cttgaaaaat ttgcaactaa gatagataca
     1921 cccgtactaa atacacttat ggggcttgga aatatagata gaaagaatga attatctctt
     1981 ggaatggttg gaatgcatgg aagtagagaa agtaatttag cactttctaa ttcagattta
     2041 gtaatagcga taggtgcgag atttagtgat agagtgataa gtaaaagttc tgaatttgct
     2101 aaaaatgcaa aaataataca catagacata gacccatctg aaataagtaa aaatatagaa
     2161 tctaatgtat ctttggttgg agatgtaaag ctagtcctta gtttattaat tgaaagggtt
     2221 gaaagtaaga ataatagcaa ttggaaagaa gagataaaga gatttagaaa gagtgagggt
     2281 gtacaaacat atgaatttca tccacaaaat atacttaaaa aaataaatga aaaatatgaa
     2341 acattaaaga aacccactgt ggtagtaact gatgtcggtc aacatcaaat gtgggctgct
     2401 aagtactgga attttaaagg taataaaagt tttataacat cagcagggtt aggaactatg
     2461 ggatttggtt taggtgcagc tattggaact aaagttggta atgtagataa aaatgtggtt
     2521 ttagtcactg gtgatggaag ttttagaatg aactgtaatg aactagctac agttgcaaat
     2581 tataatgtac ctatgcttat tttactactc aataatagaa cattaggaat ggtaaggcaa
     2641 tggcaaaagt tattttccaa tcaaagatac tctcaaactg acattaatga aaatgtggat
     2701 tatgttaaac tcgtaaatgc atataacatt gatggatata aggtatccag tatggaagaa
     2761 ttaggaaagg cgctagatat gatagatttt aataaacctg tatttttgca atgtgatata
     2821 gataaggatt atgatgttta tcctattgta gcacctaatg atgcattgga aaatttgata
     2881 tgtaactaaa acttatactg aatattgata aaatttttta ttaaattttt attagtattc
     2941 agtttttgtt gtttagagat agattggcat tttttatttt tgcaaataaa tcaatgacta
     3001 tataattaag aacttattaa attttaatta tacaatttag aata
//
LOCUS       DAYASV010000236         3009 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYASV010000236 DAYASV010000000
VERSION     DAYASV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.241"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            91..981
                     /locus_tag="ACGI68_003702"
     CDS             91..981
                     /locus_tag="ACGI68_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0140103.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAIFLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAV"
     gene            1199..2953
                     /locus_tag="ACGI68_003703"
     CDS             1199..2953
                     /locus_tag="ACGI68_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HFH0140104.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLIMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTESLNVYAIGGKSA
                     INEDLVNETKATRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKGTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
BASE COUNT         1262 a          270 c          522 g          955 t
ORIGIN      
        1 gaaataaaaa tatattataa aaacaatttg attggaattt tcagaaaatt aatgctataa
       61 ttaaaagtaa atttaaaggg aggttataaa atggctaaat taactctaga acatataaaa
      121 ggaaatacat attatattcc actccctaca atagtaggtg tatacgtaga tggtaaggat
      181 gcaattttaa ttgacagtgg aaataataag gatacagcta gacaagtttt aagattatta
      241 gaagaacata atttaaatcc taaattaata ataaacactc attcaaatgc agaccatata
      301 ggaggaaatg catatttgaa gaatcaaaca aaatgcaaga ttgctacgac taaaattgaa
      361 ggatatttta cagaaaatcc aatattagaa tcagcatttt tatatggtgg ctatccaagt
      421 aaagcactga agaataaatt tttattagca aaggaaagtg aagttgatta tattatccct
      481 tcaaatggca aaattgtaga tacagaactt gaagcgattt ttcttccagg tcattatttt
      541 gaaatgattg gtgttagaac tccagataat gttttgtttg ttgcagatag tcttacacca
      601 gagaatataa ttactaaata tcattttttc ttcctactgg atatagattc acagtttaag
      661 acactagaaa aattgagaat gttagaggct gatttctttg ttccaagtca ctctgtcaaa
      721 actacagata taaaaagttt aattaatatt aataagaaga agatggaaga aataatagat
      781 aatattaaaa aggtttgtta tgagccagtt atgattgatg aagttattga aaaaatgtgt
      841 gatttgtaca atgtcaagct tgatgctaat caatatgtgt tggttggaag tacaatacgt
      901 tcttatataa cttatttata tgagaataat atggttgaat atatatttga tggtggtaag
      961 atgatgataa aagctgtgta aatagtaata attttcaaat tatatgatga aattgtttta
     1021 gtatgactgt tatttgaaaa gtaatataaa aaaatttata taaataagct aagatgattg
     1081 atataaagtt actgataatt ttaaggtaat atatacgtta ctataatatt atgttattat
     1141 ttgtaaataa ggggataatt tataagcaaa ttaaagacct taatttacag gaggaatagt
     1201 gtatattatg aataaaaaat tcaaaaaatt cagtaaaaaa ctaagtatat taattttagt
     1261 tttgattatg tttcttttta ataatttaac tgcaaatgca cagattaaag aagattcaat
     1321 aataggaatt gatagatatg aaacagcagg acttatagca gatagacaaa cttatgatac
     1381 tgtaatatta gttaatggag ataaaaattt atctgatggg ctttcatcaa gtggattagc
     1441 tggagctatt aacgcaccaa tacttttgac taaaaaaagt gaaataccaa aagctacttt
     1501 aaatagacta gataataaga gtttaaatgt tgtaaaaaaa gtatatataa ttggtggtta
     1561 taatactatt gaaaattcag tagaaaaaga tataaaaaga aaaggtattc aagtggaaag
     1621 aataaatggt aacaatagaa ttgaaactag ttataatgta gctaaaaaaa taaatgaagt
     1681 aagtagtgta aaagaggtaa tgcttacaaa tggatttgta ggagaggctg atgctatgag
     1741 tatagcacct gtagttgcaa aaaataaagg agctataata cttacagatg ggaaatcagt
     1801 tccatttaag actgaaagtt taaatgttta cgctataggt ggcaaatctg cgataaatga
     1861 ggatttagta aatgaaacta aagcaactag aatcggagga aatgatagat ttgagaccaa
     1921 taaaaaggta atggaaaagt tttataatgg agcgacagat ttttatataa caaatggata
     1981 tcaattagta gatgcactaa cattatctcc tttagccaaa gaaaaaccaa tcgttttagt
     2041 tgctgatgga agtaataaag gtattttaaa aggagctaaa agtataacaa aagttggagg
     2101 tattgatgcc aatatatata aacagtgttt agatatgatt aaatataata atatggatat
     2161 gactccaaat attttaaaac acttaacttc agttgaagga aatgaagttt ctgaaatact
     2221 tatagaatca gataatttag gtgtggttgt tgaaaagacg aataccaata aatttgagtt
     2281 tgattacgta agtgtaacta atgaaaaaaa ttgtactttt tctgtatata aagaaaattc
     2341 ttctaataat ataaaaaatg gaaagctagt tgtaagtgct aagaaaaaaa tagataagaa
     2401 agatagtgat ttgcaagata tgaatggcga caatatgata aatgcaaata aagataggat
     2461 ggtaaatgtg ataaaaattg gagttcccga taaaatgtat tctaatttta aagtagatgt
     2521 tgaaagtggt tcagtagaac tttataatat taaaggagga gctactgttg atgtcaatga
     2581 cgggattgct aacattgttg ataacaatgt aacaaatcct ttcaatataa atactaatag
     2641 tgggatttca ggtgtaacag ctgaaactat atcatctgaa ataaaattta gagcaaacaa
     2701 tggaattgta aatgtaacag ctacgaatat aaatggcaat ataagtttat ttggaagcga
     2761 taataaaggt acctttgatg gaatatttaa attaaatctg aaaaaagaac catcaaattt
     2821 acacttgaag ttaattggaa atgggataaa taaattgcca gaaggttggt ctaaagatta
     2881 tattttagga aatggacaac caattattga agttaaaaat aatggcataa ataatatatc
     2941 aattggagaa taaaaacata tgaaaataaa gtttatatag ttttaaaaat tatagaattt
     3001 taaaatatt
//
LOCUS       DAYASV010000237         3001 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYASV010000237 DAYASV010000000
VERSION     DAYASV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.242"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            137..505
                     /locus_tag="ACGI68_003704"
     CDS             137..505
                     /locus_tag="ACGI68_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0140105.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            508..879
                     /locus_tag="ACGI68_003705"
     CDS             508..879
                     /locus_tag="ACGI68_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HFH0140106.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1024..1479)
                     /locus_tag="ACGI68_003706"
     CDS             complement(1024..1479)
                     /locus_tag="ACGI68_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140107.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1518..2783)
                     /locus_tag="ACGI68_003707"
     CDS             complement(1518..2783)
                     /locus_tag="ACGI68_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HFH0140108.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1120 a          415 c          410 g         1056 t
ORIGIN      
        1 aataaaactc aaagttatat tagtaaaaat tggaattatt tataatatac tccaaaattt
       61 aaaaataatg gttgaaaaat ctaattaaga gtactataat ttaattaagt aattacttaa
      121 tcaaaatgag gtacttatgg aaaacttggt taaaatattt aaagcattga gtgatgaaac
      181 tagacttaac atacttatat tagtatccaa gagaaatata tgtcaaaagg ggatttctaa
      241 atatctaggg atttcagatt ctgctgtttc acaacatata aaaatattaa aagatgttgg
      301 gataataaca ggatataaag aaggatacta tgttttatat catattaata aagaatcatt
      361 taatatatgt gtagatttta taaattctat gttaagtaat tctagtgaaa gctttataga
      421 tgtatttgat gtaaatacaa tacatctagg atgcagtaaa gaatgtaaat ctataaaaaa
      481 atgttgtaag agaagggaga aataataatg aaagtatgta taccagtaga ggagaataaa
      541 ggattggata gtaaaccata tggacatttt ggttctgctc caatttttgt tgtgtgtgat
      601 ttagaaagtg gagaagttaa atctttagat aatggagatt tagatcatga acatggaaaa
      661 tgtcaaccat taaaagcatt gtcagggaca gctgtagatg cagtagttgt tggaggaata
      721 ggacaaggag ctataattaa attaaatgga atgggaataa aagtttacag agcagaaggt
      781 gatactataa gtgctaattt agacttactt aaaaatggaa aattagtaga atttccatca
      841 gaccatactt gctcacatga cggatgtgga catcattaaa aaaaaacagc tgatgaaata
      901 tatacttcat cagctatttt ttcttttaat aaatacgatt ttattctttt aaaaccaaaa
      961 tttagatttt tatgtttttt gtagtatcct taataatatt tcaagtttac aagtttacta
     1021 cttttagatt ttttttgtat gtttttctat atagtcaata tcatttaata tgtcattgag
     1081 ttctttatta agtgttttta aatcatcacc atctacattg tagtttaaaa atagttcctc
     1141 tacattatca atgatagaat tgacttttct agtaacttca acaaatgtct taaagttact
     1201 actattttta gcatttagaa atttttttaa atcctttgat ataaatgata ctatttcata
     1261 ttcatcatta aatagacctc taccaatagg atatggttca attctctgta aaaaatattg
     1321 gtctttagca ttaatattat aagtataaga tatttcccaa ccgtctccta aatcaaactt
     1381 gataaaatat aatgtactac ttacttttga tattcttgtg gcaccaagct gtcttaaaag
     1441 aacttcaatc gaatcacctt gaacagtatt aatattcata tatacacatc ccctttagta
     1501 ttattatact atatttttta aaattttata attaaataaa cagtagatat aataatagat
     1561 ataatcataa gtggaaaacc tattttgaaa tactccttaa aagagatagg atgaccgtgt
     1621 ttattaccta tacctgctag tacaacatta gcagatgctc ctattagtgt tccatttcct
     1681 ccaagacaag ctccaagaga tgttgcccac catagtggca ttacatcaat accttcagct
     1741 tgcatagtca aaataagagg aattagtgtt gcaacaaatg gaatattatc taagaaagaa
     1801 gagactatag ctgacaacca taatatcaac aacattgtaa atacaagatg tccttctgta
     1861 agtccaatca atgcttgagc caatttgttt attatcccaa cttctactaa tccaccaact
     1921 actacaaata atcccataaa gaataaaatt gtagaccatt caatacctgc cataatttca
     1981 tctacatctt gttttcctat taaaagcatt atagatgctc ctgtcagtgc tacaacagaa
     2041 gaatctatgt ggattgtact gtgaaacata aaaccaaaaa gtatgaatgc tattaatata
     2101 aggcttttta taagtagagg tttatcttta acagattttt tttcatctag ttttaatatt
     2161 gcatttttag ctctttcatt cacaacaagt tcttttccat aaataaatct aaagcatatt
     2221 atagttatag caagtattac tataacagca ggacctaaat ttattacaaa atccataaaa
     2281 ctaagattgg cagcactacc aatcataata tttggagggt ctcctataag agtagctgtt
     2341 cctcctatat ttgaagctaa aatctgtgtt attaaaaatg gaactggatt aagtcctaat
     2401 atttgtgtaa taactatagt cattggtcca ataagtaaga cggttgtaac attatctaat
     2461 acagctgata aaatagctgt tataatagca aaacaaatca ttatcttcca tgggtcacct
     2521 ttggctttct ttgcagtcca gatagctata tattcaaata accccgagtt tttgacaaca
     2581 gctacaaata gcatcatacc aactaataca ccaatagtat tgaaatctat atggcttaat
     2641 ccttcatcaa gtgtaagtat attgaatatt atcattaata gtgcaccacc aacagctgct
     2701 acggatctgt ttaccttttc tgtaataata gctgccataa ctattaaaaa tatacatata
     2761 gctattattt gttgaaattc cataatataa gtacctccat ttgtattatt ttgtgcctaa
     2821 tgctagtact atatatagta ttactttttt attgtttttt caatggatgt atgaaagtat
     2881 attatttaac aaagtttttg gaaaattgac agaatttaaa gaaaattttt gttatttaaa
     2941 taaattttaa aattattcca ttgataataa tattaaacga caaacgaaaa aatataccct
     3001 t
//
LOCUS       DAYASV010000238         2996 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYASV010000238 DAYASV010000000
VERSION     DAYASV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.243"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            1195..1584
                     /locus_tag="ACGI68_003708"
     CDS             1195..1584
                     /locus_tag="ACGI68_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0140109.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            2454..2924
                     /locus_tag="ACGI68_003709"
     CDS             2454..2924
                     /locus_tag="ACGI68_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0140110.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT         1352 a          170 c          547 g          927 t
ORIGIN      
        1 atggaaaatt tataatataa ttaatgaaaa aatttaaact ttgtaatata taaataaaaa
       61 aactctaaaa tagaaggata taattatata gaatgtttca attaaacaag gtaaaaacta
      121 taatcttaaa attaaaatta acattattct taagaagcaa gaaatttaga aagtaggaag
      181 tttagtgata agtggtagta agtaagaaat ttaggaagtg ggaagtttag ttataagtgg
      241 tagtaagtaa gaaatttagg aagtgggaag tttagtgata agtggtagta agtaagaaat
      301 ttaggaagtg ggaagtttag ttataagtag tagtaagtaa gaaatttagt aagtgggaag
      361 tttagtagta ataatttata gcaagtaaga aatttaggaa gtaggaagtt tagtaataat
      421 ttgtagcaag taagaaactt agggagtagg aagtttagta ataatttata gcaagtaaga
      481 aatttaggga gtaggaagtt tagtaataat ttgtagtaag taagaaattt agaaagtagg
      541 aaatttagta ataatttata gtaagtaaga aaattaggga gtaggaagtt tagtaataat
      601 ttatagtaag taagaaattt agaaagtagg aagtttagta ataatttata gcaagtaaga
      661 aatttaggga gaggaagttt agtaataatt tgtagcaagt aagaaattta gaaagtagga
      721 agtttagtag taatttatag taagtaagaa atttagtaag tgagaagttt agttataatt
      781 tatagtaagt aagaaattta gtaagtgaga agttcagtaa taagttgtag taaataaaaa
      841 gtttaggaag taggaagttc agtaataagt tgtagtaaat aaaaaattta ggaagtagga
      901 tttactgaaa tacaaatatt taaaatcaaa gagtttaaaa gaaaggagag ctactggaca
      961 agtatccaaa atagatagct gagtacaaaa tatttcataa aattaagcaa tataatacaa
     1021 gagaattttg acattattta tatatattta ccgtgtacaa atagctaaat ttaggattaa
     1081 gattgaaaaa tatcttataa gtctgagctt atataaatcc gtataagatg tatttaatcg
     1141 aaaaatttta ttatggatat tgcttgtcta tattctgagt agcaaggttt tatcatgact
     1201 ataaaaaagt tgccacaatc agaattaaag ataatgaaat ttatatggaa atcagattcc
     1261 aaagtaacat ctagagatat tgttttagga atggagcaaa aatatcaatg gaaacaaacg
     1321 acaactttga ctcttttatc tagattagta gtaaaaagat ttctaaattc tcaaaagata
     1381 gataaatata cgcattatga agttttaatt aaagaaaagg aatacatagg agttgagaca
     1441 agagattttt ttagaaatat tcatgattct tctataaaaa gtttattgtt gtcattacat
     1501 gaaaatatta atttgagcaa agatgatatt ttacttattg aagagtggat aaaaaatcta
     1561 aaagaagagg agaaagacgt ataagcaaac gtatttctta tagcatcaat ataaagtgta
     1621 tgtaagtgta tttatgtaat atattagtct acttaagtaa tatgacaaat gattataagt
     1681 ggattatgat actataatgt gaatctaaat aaattaaata taaattaata gtggatattc
     1741 tatttaatta aatttaaaaa gctaccttta cttataagta taattaaggt agctttttaa
     1801 atagaggtag tgtaattaaa gagaattttt aaatataaat tgtgaagctt taatatagat
     1861 gataatatat attttgcaaa tatgacacag tgttttcgta tttaagatga taaaataaat
     1921 aggaattaat gatgatattg aaaaaataaa aataaaggat taatataaga tattaaagag
     1981 atagagataa aagtgttaac agaagatatt gaaaaaataa aagattgata taagatactg
     2041 aagaggtaga aacaaaagta ttaatagaag atattgaaaa aataaaaaca aaagattgat
     2101 agaaagtact gaatagatag agataaaagt gttaatagaa gatattgaaa aaataaaaga
     2161 ttgatataag atactgaaga ggtagaaaca aaagtgttaa tagaagatat tgaaaacata
     2221 aaaacaaaag attgatagaa agtactgaat agatagagat aaaagtgtta atagaagata
     2281 ttgaaaaaat aaaagattga tataagatac tgaataggta gaaacaaaag tgttaataga
     2341 agatgctaaa aaagtaaaaa taaaggatta ataaaaggta ctgaagagat agagaaaaaa
     2401 atgttaatag aagatactga aaaaatagaa ataaaaaatt aggaggaaaa tttatgaaat
     2461 atcaggtttc attattagca gtgaaggata ttgaagtatc aaaaaaattt tataaagaat
     2521 tatttgagca agaaattgaa ttggacttag gcaaaaatgt aacatttaaa ggaggatttg
     2581 ctatacaaga agacttcgca tggcttacag ggattaatcc agatacaatt atacaaaaat
     2641 caaataatat ggaactttat tttgaggttg atgattttga cgaatttatt gaaaaactca
     2701 attattataa agatgtagag tttgtccata agccactaaa acatgaatgg caacaaagag
     2761 tagtgcgtat ttatgacccg gataagcata ttattgagat aggagagtct atggaagtta
     2821 tagctagaag atatttatct gagggatact ctgtagaaga aacttctaaa ataattcaac
     2881 atccaattaa atttgtaaag cagtgtgaaa atagtaagga atgattgttt aaattataat
     2941 tatttagtag aaattaattg taatagaact aattcaagat aatatatagg actagt
//
LOCUS       DAYASV010000239         2981 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYASV010000239 DAYASV010000000
VERSION     DAYASV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.244"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(509..1828)
                     /locus_tag="ACGI68_003710"
     CDS             complement(509..1828)
                     /locus_tag="ACGI68_003710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0140111.1"
                     /translation="MLNINVFWIFLPIFSTAIEWIVLKFLLNEFSELRKNRLTLNSSI
                     VIAIMIITMLNTIEFNINIKLFICIFITYVLYKINYVVDKWKSVFISLLYWMLLIGFD
                     SIGLSIVMTLNSIKNMNDLLEDNLLKLELIMISKSLLIVLIPLLKFIRLKTKINKRDC
                     IYLSIPLIANIIGVIVIFEFIFKDKIIDSKESLIILTISTVFLLSNLSLVSIIGRIIK
                     DNSLKIEYEITKKRMNMQYKYYLNLQEYQLKIRKLYHDMNNHIICIQNVYGKNEFADK
                     YIKDINKQIKECNTIFNTQNMILDVILSDKKSICDMNNIYFLVDINFLECNFIEMPDI
                     CSIFSNIIDNAIEACIKIKDTSIQKKIKLRGTVINRLFVIKCENTKINEVVLKNNKVI
                     TSKKDSFLHGIGISSVKSSVEKYNGNVEIYSDKNKFTITIYIPLTRN"
     gene            complement(1822..1965)
                     /locus_tag="ACGI68_003711"
                     /pseudo
     CDS             complement(1822..1965)
                     /locus_tag="ACGI68_003711"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
     gene            complement(2089..2799)
                     /locus_tag="ACGI68_003712"
     CDS             complement(2089..2799)
                     /locus_tag="ACGI68_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0140112.1"
                     /translation="MYRIVICEDDITQIAFLRECILKSLEGISSQIELFEFNSGEELL
                     ETNLEGIDIFFLDIKMLQLTGMDVAKIIRETNDTSEIIFITSIVDYIQEGYKVRAYRY
                     LLKPIDFGDLNESILSCISDIIKKRENFMLIENKGIINKILINSIMYIEVRKKVLTIH
                     TKNDTYYTKNSMDKIELELEKYNFFRCHKSYLINLEYIQFICKKTVVINDEDVPVSKY
                     RMADLKKKLTYVLGDVLC"
BASE COUNT         1095 a          461 c          192 g         1233 t
ORIGIN      
        1 ttaaatcttc tacattaaaa atcatttctt cagaatctat cacaactaag tcctcctcaa
       61 attagtaaaa tatttttctt taatatttat tactaatatc aactttacta tctaattagt
      121 tatttaattg ttgtttttat gttcctagtt ataaagttta tcaaatttac attttatttt
      181 tttatactat atagaacttt tggtcaataa tgttaccata ctaaggtcgc atttgcagat
      241 aaaaataact ctatttttct aaatttattt aagatatatc atgcaaataa tataactata
      301 aaacctatca acattttaaa ttcaaacaaa tataaatcac attttttgtt ctattatttt
      361 attgagtact aaaaaggatg ttatcttatt agcatagcat tcaaccattt ttatcacctc
      421 caaatatcct atgccttaaa gatagcatct tctattttat ctttcaatac tactgctcca
      481 actgggcaat ttagagcccc aactagaatt agttcctagt taatggtata taaatagtaa
      541 ttgtaaactt gtttttatct gagtatattt ccacatttcc attatatttt tctactgaac
      601 tttttacact acttattcct attccatgta aaaaggaatc tttcttactt gtaataacct
      661 tattattttt taatacaact tcatttatct ttgtattttc acattttatt acaaataacc
      721 tatttataac agttcctctt aatttaatct ttttttgtat acttgtgtcc ttaatcttaa
      781 tacaagcttc tattgcatta tctattatat ttgaaaatat actacatata tctggcatct
      841 ctataaaatt acattctaaa aaattaatat ctactagaaa atatatatta ttcatatcac
      901 aaatagattt tttatcactt aaaataacat ctagaatcat attctgagta ttaaatatag
      961 tattacattc ttttatttgc ttatttatat ctttaatata cttatctgca aactcatttt
     1021 tcccataaac attctgaata caaatgatat gattattcat atcatgatat agttttctta
     1081 ttttcaactg atattcctgt aaatttaaat aatatttata ttgcatattc attctttttt
     1141 ttgtaatttc atattctatc tttaaactat tatcttttat tattcttcct attatgctta
     1201 ctaatgaaag atttgatagt aaaaatacag ttgatattgt aagtattatt aaactctctt
     1261 tagaatcgat aatcttatct ttaaaaataa attcaaatat tactattaca ccaataatgt
     1321 ttgctattaa tggaatagat aaatatatac aatccctctt gtttattttt gtttttaatc
     1381 tgataaactt aagcaatggt attaatacta ttagtaatga tttagatatc attattaact
     1441 ctaacttcaa caaattatcc tctaataaat cattcatatt ttttatagaa ttaagtgtca
     1501 ttactatact taatccaata ctatcaaatc caattaataa catccaatat agcaaactta
     1561 taaatacact tttccattta tccactacat aatttatttt atacaataca tatgtaataa
     1621 atatacatat aaataattta atatttatat taaactcaat tgtattcaac atagtaatta
     1681 tcataatagc aattactatg ctactattta gggttaatct attctttctt aactcactaa
     1741 attcatttaa taaaaatttt aatacaatcc attctatcgc cgtagaaaat ataggtaaaa
     1801 aaatccaaaa tacatttata tttaacataa tacatctccc aatacatatg ttaatttttt
     1861 ctttaaatta gccattctat atttactaac tggtatatct ttaccattaa taactacagt
     1921 atttttacat ataaattgaa tatactccaa attaattaaa tagctcttgt aacattttta
     1981 aaaatttatt ctttcttaaa tcactgaatt catttgataa aaattttaat acaattcatt
     2041 ctatcgccgt agaaaatata ggtaaaaaaa tccaaaatac atttatatct aacataatac
     2101 atctcccaat acatatgtta attttttctt taaatcagcc attctatatt tactaactgg
     2161 tacatcttca tcattaataa ccacagtctt tttacatata aattgaatat actccaaatt
     2221 aattaaatag ctcttatgac atctaaaaaa attatatttt tctagctcta attctatttt
     2281 atccatacta tttttagtat aataagtatc atttttagta tgtatagtca aaaccttttt
     2341 cctaacctct atatacataa tactattaat taaaatttta ttgattatac ctttattttc
     2401 tatcagcata aaattttctc ttttttttat aatatccgat atacaactca aaatactttc
     2461 atttaaatct ccaaaatcta taggctttaa taaatatcta taagccctta ccttatagcc
     2521 ttcctgtatg taatcaacta ttgaagttat aaatattatt tcagaagtat cattagtttc
     2581 tctaattatt ttagccacat ccatgcctgt tagttgaagc attttaatat ctaaaaaaaa
     2641 tatatcaata ccttctaaat ttgtttccaa taattcttct cctgaattaa actcaaatag
     2701 ttcaatttga ctagaaatac cctctaagga ctttaatata cactctctta aaaaagctat
     2761 ttgtgtaata tcatcctcac agataactat tctatacata aacacccctc cttagacatt
     2821 agatctgaaa acaaataaaa tatcaattta taaataatta tttagttata caactaacat
     2881 ggtcatcaaa tactattatt cacttaaaaa atcaccccct atttttagta tgttaacaac
     2941 aaaataagaa gatatcttag atttttaaaa acaattaata c
//
LOCUS       DAYASV010000240         2897 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYASV010000240 DAYASV010000000
VERSION     DAYASV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.245"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            351..2486
                     /gene="rnr"
                     /locus_tag="ACGI68_003713"
     CDS             351..2486
                     /gene="rnr"
                     /locus_tag="ACGI68_003713"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFH0140113.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1272 a          271 c          522 g          832 t
ORIGIN      
        1 agtatactaa tagaataaat tattatatag taatacttat atgagatgaa tattttacca
       61 atcctatttg atggtgtata attaggtgat aaagagtaat ttaagcaatt atgataaaga
      121 ataacttaag taattataat gatatttaca aataatttat aaaacatcat aatttgtttg
      181 aagttagtat gacaaagctt acattaaatc aaaaatataa ataagtaatt ttaacttata
      241 ataataagaa aatatagaaa attatgtgat aattaaaaaa tgttttgtct gaagaaatat
      301 aaaagacaat ataaataata atataatgaa aagtaaaaca ggagggaaaa atgataccag
      361 gacttaaaga aagtttgcta ggattaataa atgaaagtgc atataatcct ctaaaaaaag
      421 aagaattagc agaaatattt gatatacatt catcagaaat gccaatgttt tataattttt
      481 taaatgaatt agaagaagat ggttatatct gctttactaa aaaaggaaaa atagtatctc
      541 ccaatcaaat gggatacttt gttggaaaat ttgtatcaca caaaaagggt tttggatttg
      601 tagaatctga tgttgaatat acacaggatt tatttatctc aagtgacaat ataaatggtg
      661 ctatgcataa tgatagagtt atggcagaaa tagtagtacc tgcaactgaa gaaaagagag
      721 cagaaggaag aatagtaaaa gtaataaaga gagaaataac tagaatagta ggtactttcc
      781 aatcaagtaa aacttttgga tttgtaacac cagacaacaa gaaatttact aaagatatat
      841 atatacctaa aaaacatttt agtggagcag tagataatga taaggtagta tgtgaaatta
      901 cagtatggcc acaagaggat agaaaaccag aaggcaaaat tatagagata ctaggtcaaa
      961 aaggtgaaag aggcgtagaa atagattcta taataagaga acatggtctt ccagaagaat
     1021 ttccaaagaa agtattgcaa gaagcagaag ctgtagctgt tgagattcct caagaagaga
     1081 taaaaagaag aagagattta agagatttaa acatatttac tatagatgga gatgacgcaa
     1141 aagacttaga tgatgccata tcaatagaag tattacctaa tggaaacttc aaactaggag
     1201 ttcacatagc tgatgttaca cattatgtaa gagagaaaaa taaacttgac aaggaagcct
     1261 taaaaagagc tacatcagtt tatttagtag ataaagtaat tccaatgttg ccaaaaactc
     1321 tttcaaatgg agtatgtagc ttaaatccat ttgaagataa attaactctt tctatattta
     1381 tggaaataga ccataaagga aatgtggtta aacatgaaat atgtgaatct gtaataaatt
     1441 caaaagctag aatgacttat acagaagtat ctgatatatt agaaaaggat gatgaaaaac
     1501 ttaaaaagac ttttgaacat gttgttgaag attttaaaaa tgctgaaatt ttagctagga
     1561 ttcttatgtc aagaagggaa aaaagaggag ctatagactt caatttccca gaggcaaaga
     1621 taattctaaa tggaaaaggc gaagttgtag atataaagcc atatgaaaga agaatatcta
     1681 ataagataat agaagaattt atgcttataa gtaatgagac aattgccgaa catttttact
     1741 ggatgggaat accgtttgtg tatagaatac atgagacacc atctttggaa aaaatggaag
     1801 aattgagtaa gtttatatct acatttggat atataataaa aggtgataaa gaggaagtac
     1861 atccaaaagc attacaaggt ataatagaga aaataaaggg caaaaaagaa gaaggagcta
     1921 tcagtactat aatgcttcgt tctttaaaac aagctaagta ctcaccagaa tgtagtggac
     1981 attttggatt agcagctaaa ttctactgtc actttacttc accaataaga agatatcctg
     2041 accttcaaat tcatagaata ataaaagaat ctttaaataa taaaataagt ggaaaaagac
     2101 aagaacagct tactactata gtggattatg catctgttca atcatccgag aaagaaagaa
     2161 aggcagagtt agctgaaaga gatgtacatg atttctataa ggctctttat atgaaggata
     2221 aagttggtca agaatttgag ggtgttgtat ctagcgtaac ttcatttgga atatttgttg
     2281 agttggataa taccatagaa ggattgataa gacttgcaaa tatgaatgat gattactata
     2341 tttatgatga gagttcttat agtgttttag gagaaagaac taagaagtca tataagatag
     2401 gagaccttgt taagataaag gttgaaagtg ttaatgtaga ttttaaagaa attgactttg
     2461 agatagttga aaaaatagag gagtagataa aacagctaaa ataagagata acaagttgta
     2521 gtatttatga tatgtataaa tatatagtgc acaatactct ataaaataaa tgataaaaat
     2581 aggtaattga gaaatatcta atggaaatag ttaactgaaa gataaataat taagaaatat
     2641 ttaatgaaag tagctaattg aaaaataagt aattgagaaa tgtttaatag aaagagctaa
     2701 ctgaaagata agtaattaag aaacatttaa tagaaaaagc caactgaaaa ataaataatt
     2761 aagaaatgtt taatataagt ggctaattga aaaataagta attaagaaat gcttaatata
     2821 agtggctaat tgaaaaataa gtaattgaga aacatttaat agaaaaagct agctgaaaga
     2881 taagtaatta agaaata
//
LOCUS       DAYASV010000241         2816 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.246, whole genome shotgun sequence.
ACCESSION   DAYASV010000241 DAYASV010000000
VERSION     DAYASV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.246"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            134..418
                     /locus_tag="ACGI68_003714"
     CDS             134..418
                     /locus_tag="ACGI68_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140114.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(575..748)
                     /locus_tag="ACGI68_003715"
     CDS             complement(575..748)
                     /locus_tag="ACGI68_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HFH0140115.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1189..1404)
                     /locus_tag="ACGI68_003716"
     CDS             complement(1189..1404)
                     /locus_tag="ACGI68_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140116.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1415..1882)
                     /locus_tag="ACGI68_003717"
     CDS             complement(1415..1882)
                     /locus_tag="ACGI68_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFH0140117.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMIISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            2226..2324
                     /locus_tag="ACGI68_003718"
     CDS             2226..2324
                     /locus_tag="ACGI68_003718"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140118.1"
                     /translation="MFILKRLCKLVSINKSFSEEIPKNLKNTYMCI"
BASE COUNT         1150 a          270 c          333 g         1063 t
ORIGIN      
        1 aatttaaagc attaaaaaat aaatttgtat tttgttttat ataaagtaat ttgaagaata
       61 tggaaaaact tgattagtac attattaatt agggtataaa atggtgtaaa tagaatgata
      121 aggggtggat gaaatgcttg tatataataa aagtttttat cctaatgaca tatttccaag
      181 attagatttt tcaaaaataa aaaaacagtt aaaattgata gataatgacc tgtcagattt
      241 tggaagcata tgtataatag aaaaagaaca ttatacaata agtgtaaaca gtataggtga
      301 aataaatgtg tattatgatt tagagtacga aaataaggtg tatagaatag tttatgagat
      361 tgaaaagtta tttaaatctc aagttggaag gtttagcata tctacataca gaaattgata
      421 attaaaaaag tagaaattaa aaaacttaat actaaagata tagataaata tatgaggtaa
      481 cagctagaga gagttgttac cctttttaaa tactaagtat atattattct tattaatttt
      541 ttgtaaattc actaaaagac ctttttatag aatattattt tcttttttta ttaaataggt
      601 aaatcagtaa aaatgcaatt ccagttccaa ttattattcc aatgatattt tctataatta
      661 atttttttaa tataaaagta aataataaaa atgctataca taaacctaaa atatatcctt
      721 taaaataggt tgaaagtttt cttttcatat aatcaccaac ttgtatcaga tttagtttac
      781 ttaattagag tataacagtg ttgtaacttc aaagagtatc taaaagatta caaatgttag
      841 ataaaatcat caataatgta atttataaca cgtagctata aactatgcaa taatataaat
      901 ggaggtataa gtaaatgaaa aagttccatg ttttgctaac agtttaaata aatattaggt
      961 agaaaagaat gtatatagga atctgtggta aaattatcaa tttaaaagta ttatttagtg
     1021 tgatacagtt cttataggta atggataaca aatagataaa ggaacagttt ctttcttaga
     1081 acatattttg ttatgacttt aaacaaagtt aatattaatg tcaataatat agtaaaagaa
     1141 taaaaatcat atagtataat aaaaaagagt gtttctaaac actctttttt aatcattttc
     1201 atcttctaca tattcaagta tatcacctgg ctgacagtct aaagctttgc aaatttcgtt
     1261 tagagtggta aatctaatag cttttgcttt tccagttttt aaaattgata aatttgcatt
     1321 tgtaatatct attttttctg ccaaatcttt aagtgatatt ttcctttttg ccatcattac
     1381 atctaggtta actactattg ccataacatc acctctatat cgttaaatca ttatcttctt
     1441 ttatttttat agctttatca aatgtttctg caattacaaa gcacattagt gcaaatataa
     1501 aagagataat cataagtggt gagataccgc cactatcagt tccaaaaatt cttatattat
     1561 tagtatttaa attataacca ttgatacatt caattatagt gtttatcaat aaacaaaatc
     1621 ccattacctt aaaccttttt acattttcaa gtacgaatgg ggaatactta gtactattaa
     1681 ttataaatat caagaataag atgactataa agtagtcaga aaaatatagt attcttgcaa
     1741 ttaaagcgat tactttgtca gaactaaaat catataaaaa cataggtatc atacataaga
     1801 atattatacc agcaactcct aaaagtgata atagaaaata tagaaatact gataatatat
     1861 tactaaaaaa ttttgtaatc ataaaatatc atctccttta tataaagtat attattgatt
     1921 ataatataca atttattgaa tttcaataat aaattattga tataatatat aaaaccattg
     1981 taatatacac taaaaataag aatgtataag gtggtaaatt tatataaaaa agaatatgag
     2041 ttgtaaaata ttaaataata ttgtactaat tggaataatt ttaactttat tatagattat
     2101 tttaatacca ttagtactga tggtgtattt taaaattggt ataagcatta caaatacgaa
     2161 tatgccaact gtaatatgta atataagtag ctatatacat atgtgctgtc ccatatattg
     2221 tatcattgtt tatattaaaa agattatgta aattggtatc aatcaataaa tcattttcag
     2281 aagaaatacc taaaaactta aaaaatacct atatgtgtat ttagttagat ttgagatttt
     2341 tacatttaat tttatagtga caatattata ttatttttat gatgtctatt tatatgcaat
     2401 tactataata tcagaactta tagtatcatt tgtttcatta tcaataaggt tttttacttt
     2461 agttttgtca attttattaa aaaaagttat tgatataaaa gataagaagg ataaaactat
     2521 atgacggatt tcaaacataa gagtggatat ttatataata gatattttat agttaatttt
     2581 attagatttt atttaatact gtaatattaa tatcacaata tattaatagt gtattaaaga
     2641 ttgaattttg tagataacta taatttattg agagtgataa ataaaaatta tataatatta
     2701 cagaagaaag agagggagac aatattatat ccctcttttt tctataataa ctaattatta
     2761 aaaaattatt aatactctat atacttttaa tttctttaat aataactaat ttattc
//
LOCUS       DAYASV010000242         2799 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYASV010000242 DAYASV010000000
VERSION     DAYASV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.247"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(337..1233)
                     /locus_tag="ACGI68_003719"
     CDS             complement(337..1233)
                     /locus_tag="ACGI68_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0140119.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASHVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYNI
                     SPTKYRNEHKEVSNVILKEDMK"
     gene            1374..>2799
                     /locus_tag="ACGI68_003720"
     CDS             1374..>2799
                     /locus_tag="ACGI68_003720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0140120.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIGKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFDWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
BASE COUNT          962 a          383 c          326 g         1128 t
ORIGIN      
        1 gatggattta aattttctat aaaaatcata ctattttagc tgtttacttg cttatttaat
       61 agttttattt ctttgattaa atatctttca gaatctaagc cagatgtctc aaaaaaactg
      121 atttataata taatcatttt ttctgctatt cttatttttc caattttagt ttcatattaa
      181 aatatacttt tcatagacac tacattcttt catactcatt ttcacattta actatacaag
      241 tagtcttact catttatgta ctatacttta ttaatattta ttaagaaaaa ctcaaagatt
      301 taaaaatata tattttctat aaagtatatt tatgaattat ttcatatctt cttttaaaat
      361 aacattacta acttctttat gttcatttct atacttagtt ggagatatat tataattcct
      421 tttaaataag cgactaaaat aatttatgct atcaaagcca catctcatag caatttctgt
      481 catatttaaa tctgtttcct ttaaataata tatagcttta tcaagcctga ttccatttat
      541 aaaatcgatt gtagtcttac ctgttatttg cttaaatgtt ctacaaaagt gatgtgcact
      601 caagttcaca tgactagcaa gttcttttaa tgtaatttta tccatatatt tttcttctat
      661 caaatcaaag atacattcaa atttttctaa agaatacttt ctttttataa attcatcttc
      721 agaatatatt ttatcaatat gacttcttaa taagagtact attaattgat agatattagc
      781 ttttatagat aactcatatc caacttcttt attgtgatat tcatcaagta tagttgtaat
      841 acattttaaa atcagagaat cattttgaat taaattatta aatacaatat gatttaaagc
      901 caatggtgct aagtacttgg tttgtaacaa atcaacttga ttactaaaaa ggaatgtggg
      961 ttcaatacga atactataga attttaaatt atctgataag ctttctaaat aatgtaattc
     1021 attactgttt gctattacaa tatttccagg cactacctta aaattatcca atccacattc
     1081 aagatatgct ataccatcaa taaaataata tatttgtaga tgctcatgcc aatgtctttt
     1141 aaagtttgct ccttttttaa tgtcatcctt taaaaaaata tctataaagt tttcttgacc
     1201 tttcctgtta aaatatgtat ttttatcctt cattattccc ccctcatcaa aatagtgcta
     1261 attataagca atatagttat tgtattcatt atttcatatc gctattatat aaaatgtaac
     1321 tatcattgta aacattaaca taatgcagtc attataataa ggaggtaata aaaatgacaa
     1381 actcttttaa aaaagatgat aatagaaaaa aaatcacagt tttatttgta gtcattgcca
     1441 tgacttttat ggcaacatta gattctagta ttataaatgt tgctctccca gttcttgcaa
     1501 gcaaactaaa tgtatctcta gcatctattg aatgggtaat agcaagttac tcaattatta
     1561 tttgttcaac actattattt tttggaagac ttggagatat tattggtaaa tcaagagtat
     1621 ttcaagtagg tactattctc tttacatcag cttcattact atgtggatta agtaattctt
     1681 taactttact tatagtttgc cgctttattc aaggaatagg cgcttctgcc tacatggcaa
     1741 acaatcatgg aattatcaca gaactatttc caaaagaaag cagaggaaaa gccctaggga
     1801 ttttagttac tgcagttgct attggaaata tggttggacc atctgtggga ggatttattt
     1861 tgtcaatatt tgattggaat gttatatttt ttataaatat tccaatcggt cttattgtaa
     1921 tctttttaaa tacaaaattt cttcctaata gtaaaaaatc ttcagaaaat atggataaaa
     1981 ctggtgctat acttcaattt ttaggaacaa cattattttt tagtgctcta attagtgctc
     2041 aacaaacagg attactaaat ccatatatat taattgcact attattatca attattttta
     2101 ttatattatt tttgatatta gaaaaaaaac atcctcaacc gcttcttgac cttgaaattt
     2161 ttagaaattt taaattttca ttgaatttaa tttgtgctct tacatcattc atttgtattg
     2221 catcatcaag tattttaatt ccattttatt tacaaagtac tatgaaactt ccccctattc
     2281 aagcaggttt atttatgata ctatcacctt tgattttagc cattttttca cctatatttg
     2341 ggaatatatc agataaaatt aagtcagaaa aaattatttt aattggtctt ctcgttatga
     2401 gttttggatt ctttctaatg tcaagattga aagaaagttc tgcccttatt ttatttgtaa
     2461 tttatatatc gataatatca ataggacaag ctatatttca acctgctaac aatgctttga
     2521 taatgtcaag ctgttctaga agtaagctag gagttgtagg aagcataaat tcccttgtaa
     2581 gaaatttagg acaagttata ggtattacta tttctacaac acttttatat aattttatga
     2641 gtattaaagc tggatatagg gtcaatgatt atgtgattaa taatgataaa atatttgtat
     2701 ttggaatgag aaatgtttat attattgtta cacttgtttg cttaataggt gcaatattaa
     2761 ttggctttta tttatttaaa tataataaaa atgaacaat
//
LOCUS       DAYASV010000243         2793 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYASV010000243 DAYASV010000000
VERSION     DAYASV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.248"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..597
                     /locus_tag="ACGI68_003721"
     CDS             <1..597
                     /locus_tag="ACGI68_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140121.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            694..1731
                     /locus_tag="ACGI68_003722"
     CDS             694..1731
                     /locus_tag="ACGI68_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0140122.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2540..>2793
                     /locus_tag="ACGI68_003723"
     CDS             2540..>2793
                     /locus_tag="ACGI68_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140123.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT         1064 a          361 c          426 g          942 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaaatt actttagata gtactactat aacaaattta
       61 gctgagtata aaagaactca aatagtaagt agagtttttc aagacccaag ccttggaact
      121 tgtccttcta tgacggttag agaaaattta tctctagcac ttaataaagg gaaattattg
      181 aacttaaaaa aatgtcttcg tcataaaacg aatgacttgg aacacttact tgaaggaatt
      241 tctttggatt taaaaaaata tttagatatt aaagtacaat atctttcagg gggtcaaaga
      301 caatcactat cactaattat gtctagctta gcaagtccta aggtcttatt actagacgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa tagagttaac agataaaatc
      421 gtaagagaga aaaatataac tacacttatg gttactcaca acttgaaaca cgctcttcaa
      481 tatggagata ggttgataat gcttcataag ggtgaagttg tattagatgt aaaagataaa
      541 gaaaaaaaag atttaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtataagaa
      601 attaaaatct tagttttaat cagaaatatt tataatctat catatgaata tatgaacaaa
      661 tataattatt aaattttaag ggaggaaaat aaaatgataa aaagtaaaaa aatattaagt
      721 ttaattatag caggagtact tggtgtatca atgcttactg gatgttctca aaatgatggc
      781 tccaatgctt caaatgaaaa taaagaaaca gatagcaaaa aacaaaaaaa tataggtatt
      841 actcaattag tggaacatcc atctctggat aaagcaaaga aaggattcat caaagcactt
      901 gaagataaag gctataaaga tggagataat ataaaaatag atttccaaaa tgcacaaaat
      961 gatatgccta ctacacaaag tattgctagt aagtttgtat ccgacaaaaa agatttgata
     1021 tatgctatat ctactccatc tgcacaagca gcttataatg ctactaaaga tattccaata
     1081 ataatgactg ctgttacaga ccctgtagaa gctggacttg ttaaatctct tgaaaaacca
     1141 ggtggaaatg tttctggtac atctgattat ctttcaattg ataaaacatt agaattggtt
     1201 aaaacattga ctccaaaagc aaagaaaata ggggttatat acaatactag tgaagttaat
     1261 tcaaaaatcc aagttgattc tctacatgat tatgctaaga aaaataatta tgaagtagtt
     1321 gaaaaaggaa tcagctcttc aagtgaagtt aaccaagcta tttctagttt agttggtaaa
     1381 atagatgttt tatatgtccc tactgacaat ttaatagttt cttctatgcc aatagtttct
     1441 aaagttgcta atgaaaacaa gattcctata atagcctctg aagaaggttc tgtatcttct
     1501 ggtgctttag cttgttgtgg aatagattat gaaaaactag gttataaggc tggagaactt
     1561 gctattgaag tattagaagg aaaatctgtt ggcgatatac cagttactac attagatgaa
     1621 actgaaataa taattaacga agatacacta aaagcacttg acatgcaaaa gttatcagca
     1681 gataatataa agtatataaa gtcagatgaa aatgcaaaat ctacaaaata aaacttatta
     1741 atttcaaaaa ataggtaata tgaagtaggt aatatgaaaa ctttatataa taagtactct
     1801 tatccaccct aaccctttga aaattgtgct tattgtagca atttgaatat tgcatataaa
     1861 gttattcatt ttccataaaa gtcgtctata ttgacgactg attatgaaag ttatataact
     1921 ttctaatcac tacttgagct gtccaaaaga attttaatat tctccagaca gctttttttt
     1981 atgatatttg attgatatac atgtctagtt attccatatt tagtaaatat tttgataaaa
     2041 tttccacatt cacttataac ttaggataac gtttttttca aacaatacat taatttaaaa
     2101 gtattctgag tatacattat ttagtatttt aattaaattt agcagttgac ttttattatt
     2161 gtataattta taatttataa caagtcgaga atacatataa ttttctgact aattaaaata
     2221 ttaaaaggga gagataatta tgaatacttt attaaacaat agaaaatatt attattatta
     2281 tagccactat ttttagggtt tgtacctacg ttttaacata gatacagacc ccttaagact
     2341 actaaaattt tgggattgta tctatgttgg ctatattgtt tataaagttt tcatctaact
     2401 atataagagc cacatagatt aaactatgtg gcttattcgt gtttttacca catagttttg
     2461 tacactatgt ggttttttta tacacaaaat tagcaaataa ttaaaattat aaaaatattt
     2521 tcaaaggagt gttttattta tgtcaggtat tatatctgta atgacgcaaa gtttaatttt
     2581 atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc cagatatgtc
     2641 tgctgatggt agctacacaa tgggtgcttc tattgtagca ttttctctta ctaatggtat
     2701 ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg gtcttgttac
     2761 agggattctt cacattaagt ttaagatatc taa
//
LOCUS       DAYASV010000244         2737 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYASV010000244 DAYASV010000000
VERSION     DAYASV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.249"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            155..1087
                     /locus_tag="ACGI68_003724"
     CDS             155..1087
                     /locus_tag="ACGI68_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HFH0140124.1"
                     /translation="MGFIGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKT
                     KIYSNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSK
                     KAGALIYSIHPIFAFSNKNTDIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRD
                     KDTSSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNG
                     FTKALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNIT
                     IENAIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1062..1889
                     /gene="panB"
                     /locus_tag="ACGI68_003725"
     CDS             1062..1889
                     /gene="panB"
                     /locus_tag="ACGI68_003725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFH0140125.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIIKDSIKNYILEVNTGAFPQEKHSFSINESELEKLY"
     gene            1908..>2737
                     /gene="panC"
                     /locus_tag="ACGI68_003726"
     CDS             1908..>2737
                     /gene="panC"
                     /locus_tag="ACGI68_003726"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFH0140126.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSIFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     T"
BASE COUNT         1088 a          333 c          454 g          862 t
ORIGIN      
        1 tttatgaaaa tggtaagata tttatggatg gtatcttgtt ataagaacta tactttaaat
       61 gtttttaatt attttgtctt atgtaactca tatcctcagg aatgagatta gaaattaaga
      121 tgttgtatca caaagtggag gtgatactat taaaataggc tttattggtt ctggaaaagt
      181 tggaacatct ttggcaaatt attttttatc aaaaggttat tgtgtttcag gcttttatgg
      241 aaggaatcaa ttatctttat tagaatcaac aaatttaaca aaaacaaaga tttacagcaa
      301 tttgaaagat ataatttatg aaaacgatat attatttatt acaacatcag atgattctat
      361 tgaaattata gacaaaaaac tttccaaatt taatttaaaa cataaataca tttgccacac
      421 aagtggctct ttaaaatcta gtatattgtt ctgttcaaaa aaagcaggtg cattaattta
      481 ttctatccat cctatatttg cattttccaa taaaaatact gacataaaaa aaatgaaaga
      541 catttgtttt tccatagaag gtgataatga acaagatgat ttagttattc aaaatttcat
      601 agatgatttg ggaaaccaat tttttataag agataaggat acttcatcca cttatcattt
      661 agctaatgtt ttagtttcaa atttagtatt atctttacta gatataggag taagttactt
      721 tattaaatta ggtttatctg aagaggaatc tttaaaagct atcagtcctt tagtaaaaaa
      781 aaacatagaa aatatacttg ataatggctt tactaaagct cttactggtc ctgtttctcg
      841 tggagatata acaccaataa ggaaacatct atctgtattg gataaaaaag atgaggatat
      901 ttacaaaata ctatccttaa acctactaaa aataattgcc ttaggaaatg acaattcatt
      961 aaaaggtata aaaaatatta ctatagaaaa tgcaatcgaa aatttaataa gtaaatctga
     1021 aaaatacaaa gaaatttaca aacttttagg aggaaaaaac gatgaaaaat accatacaga
     1081 cttttaagaa tgctaaatat gaaggaaaaa aactatctat gcttactgct tatgactact
     1141 caattgcaaa aataatggat gagtgtgata taaatggtat tttaatagga gattcattag
     1201 gaatggtcat aaaaggtgaa gaaaacactc tatctgttac catagacgaa attatatatc
     1261 atacaaaagc tgtaaaaaat ggcgtaaaaa atgccttaat cgtaagtgat atgccttttc
     1321 tatcttacca tgtttctata gaagatgcag ttaaaaacgc tggtagactt ataaaagaag
     1381 gaggagctca tgctgtaaag ttagaaggtg gctcaaacgt tataaagcaa atagaaagca
     1441 ttgtaaatgc tcaaatacct gtaatggggc atttaggatt aactcctcaa tcagtaaatt
     1501 catttggtgg gtttaaggtt caaggaaata ccagtgaaac agcaagacaa cttattgaag
     1561 atgctaaatt aatagaaaaa gcaggtgcat tctctattgt ccttgaagga gtgcctacga
     1621 aaatagctga gatggtaact aattctattt caattcctac aataggtata ggggcaggaa
     1681 ttaactgtga tggacaaatt ttagtttatc aagatatgtt gggaatgttt ggagattttg
     1741 ttcctaagtt tgtaaagcag tatgcaaata taggagatat aataaaagat tcaattaaaa
     1801 attacatcct agaagttaat acaggtgcat ttccacaaga aaaacacagc ttttcaataa
     1861 atgaatctga gttagaaaaa ttatactaat attaaggatg gtacaaaatg cttgtaaaag
     1921 aaattaaatt actaagaaat ataatcaaag attggagaaa acatggctat tctataggcc
     1981 ttgttacaac aatgggattt ttacatgaag gacaccaaag tcttataaaa aaagctgtaa
     2041 aagaaaatga taaagtagta gtgagtatat ttgtaaatcc aactcaattt ggaccaaatg
     2101 aagattttaa tagctatcca agagatatag ataaagattt taaatattgt atggatagtg
     2161 gagcaacagt agtctttaac ccatcaccag aagaaatgta tttaaaaggt aattgtacta
     2221 ccattaacgt ttctggactt acagattttc tttgtggagc taaaagacct gttcattttg
     2281 gaggtgtctg cctagttgtt tcaaagtttc taaatatagt aacacctgat aaagcttatt
     2341 ttggagagaa ggatgcccaa caattagctg taattaaaag aatggtcaag gatttaaaca
     2401 tagatacaga aataataggc tgtcctataa ttcgtgaaaa tgatggatta gcaaaaagtt
     2461 cgagaaatac atatctctct gaggaagaac gaaaatctgc tctaatatta aataagagct
     2521 taagtttagc aaaagaagaa ttagtaaaag gcaatctaaa tccagagaat ataaaagaat
     2581 taataacagc taaaataaat tctgaacact tagcaaaaat agactatgtt gaaattgtag
     2641 atagtgagac attacaacct gttaagcaaa tagaacattc tatacttgtg gcaattgctg
     2701 tgtttatagg aaaaaccaga ctaattgata attttac
//
LOCUS       DAYASV010000245         2718 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYASV010000245 DAYASV010000000
VERSION     DAYASV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.250"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..628)
                     /locus_tag="ACGI68_003727"
     CDS             complement(<1..628)
                     /locus_tag="ACGI68_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFH0140127.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            complement(821..982)
                     /locus_tag="ACGI68_003728"
     CDS             complement(821..982)
                     /locus_tag="ACGI68_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140128.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            complement(1324..2484)
                     /locus_tag="ACGI68_003729"
     CDS             complement(1324..2484)
                     /locus_tag="ACGI68_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HFH0140129.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
BASE COUNT          979 a          471 c          267 g         1001 t
ORIGIN      
        1 aaatgtaacc tattacagga taatggtctg acaggtaaat tttttctact gtgtatcctt
       61 ttagttcagt atttttatta acaaaaatgt aatctattct agagtttgat ttttcaaatg
      121 ttggtaagta agacttgttt agtgaaatag ccatatcatt aaacatactt aaaaagacat
      181 ttttctcatt aaaatcacca cataaaataa ctatccccac taatctgttc ctatagtcta
      241 ttatttcgtc taattgtttt gctctctcct gcctatcaag tcccaagtga gtatttatta
      301 catttataat tcttccatat gcatctatag ttatacaaag agctccccgt tgttcttttt
      361 tgctagttaa gaaaaaatga ttactgttta acattttaga tgttgtaaat gtacaaatac
      421 catatatcat atttactttt tttacatttg tcgcaaagac accatccata tttaaaacag
      481 cttttaaagc taaaaattga ggatataata cttcttgcaa acaaattaca tcacaatcca
      541 attgttttaa atataacccc attttagtta gagttaatct attatttgaa tccatacctt
      601 tatgaatatt gtaggttact attttcataa taatatcacc tcttatgcaa tataatactt
      661 actaaaattt aaacttattt acttttcact taagaccatt atttatctta taaaattgag
      721 tacttataaa ctttatcaca aaaaaaccta acttaagtat ttatattcta cataagttag
      781 gttttataaa ctattataat atttattatt tcattcattt ctaatgatga ttctcctttt
      841 ttatcttttt caatttgcct tcactaacaa caaataaaaa tcccatcttc ttgtatattt
      901 catccaattc aattaaagtt acttcaccat tttttaaatt tgttaatgtt atatcttgta
      961 ttcttcttga tggaattatc ataagttatc aacactcctt taaattcttt ttaatcttta
     1021 tatttaaata tatttatgca ttctgatata tattcataaa ttattaaaat aaattcaaaa
     1081 aacacccgtt ctgcaaacat tcgttctgta tatttattat acatcataca gacgttcgat
     1141 tcaatataaa aattaaatat atctcgtttt tagtaaattt tacaatatta tacactaaat
     1201 atgtcaaaac atcttcttga aatagattat cttgtcaaac aaaaaagcca tttcagtaaa
     1261 agcttctgct taaattgaaa tgacttttct tatttaaatt attaatttta taccatattt
     1321 ttattaagct aacatatgta atatttgagt taccataaga gcacaataag tccctataat
     1381 atttcctaga agccccataa gtacacctac tggtattaat acaggattgt gaaccccagc
     1441 aatcattgga gctgtagtta ctcctcctat attagctata cttcctactt cacaagtaaa
     1501 taaatcaagt ttaaatagtt tagcaagccc tataaatgaa atagcatgta caaggactac
     1561 taccaatcca aacattatgt aaacaggtgc ttctgacaat ccaaagaaat ttgctttact
     1621 acctgtagct gcaaggagta aatacataaa cacatttcct accagtgtac tccctttaag
     1681 ctttgatatt tttgtcatac ccaataatat tgatactgta gatgcaacaa gtactaccca
     1741 catagtttca ttaagtatac ctgaagttgg aagtatcttg gatacaattt gtgcaaaaga
     1801 cgcaactcca aacccaacag aaagtaaaat cataagttct acaaacgtag gtgctgtatt
     1861 atctttattt tctttttcaa gttttgatgt aacctcatct ataaaacttg tatcgcactt
     1921 tgtccatttg ttaaatttag gtccaaattt cacagacatt agacaaacag acatccatac
     1981 tgtatatcct atagaaccca taagtattgc ataactaagt ccttcttctg caactcctag
     2041 agcattagct gctgcaatca tatttgatga gccacctatc catgaaccag atgctgcccc
     2101 aagaattttc catgaatctg cacccattga atttttaaaa attatgaatg ctataacaaa
     2161 tccaagcata aatgtaaatg tagcacagaa aaatgataat aacattttag gccccatctt
     2221 taatattttt cttaaatcac attgaattaa caaaaacact aacattaatg gaaggaagtt
     2281 cactgttatt atgttttgat acgttccaac atcttcattc tgtgcaaata aaccaaatga
     2341 tccaagtata gctgaaccta agtatattaa aagcattgga ttaataacat caaatacctt
     2401 ccatttcaat ttatcaacca taaaaaaaat tacacctacg aatacaacca agaaactaca
     2461 atatacaaat ccagtctcaa tcacattata cacacccttt cttttcctaa ttaatttgat
     2521 ttttatgatt tctaagtaaa gactttttca gataatagac aattaaaata aatactttta
     2581 tctatatcat gctaattaaa tacatatata cctaactatt gacatatatt aataaagatt
     2641 tgcacattaa ttactttaga aatgaataat ctgaatttaa aatctttttc aacaattatt
     2701 ttattactaa aattttta
//
LOCUS       DAYASV010000246         2656 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYASV010000246 DAYASV010000000
VERSION     DAYASV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.251"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            511..1386
                     /locus_tag="ACGI68_003730"
     CDS             511..1386
                     /locus_tag="ACGI68_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0140130.1"
                     /translation="MLVYYLYNLIFLEGGVITLKLKIVGSGGMSIIPSSFCKCKICEE
                     ARQKGGRYERLGPSLYIDDIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGI
                     RIVEKIGCDFITGENNPIGFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKI
                     NNFDIDLINNNPDRNITFYVIKENEKKVIYACCNPKPFTHSDMYFDADILIISLVSDD
                     GILNDGTKLKDTPFKDEIFTLDEIVEIKNYYRIKKIIVTHIDEMWGKSYGYYSELEKK
                     LDNIYFAYDGMEIIV"
     gene            complement(1696..2475)
                     /locus_tag="ACGI68_003731"
     CDS             complement(1696..2475)
                     /locus_tag="ACGI68_003731"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFH0140131.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDVVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT          973 a          378 c          311 g          994 t
ORIGIN      
        1 aattatgatt ttatgtttaa atgactagaa tttgattagg atattatctt aatttgtcag
       61 ataaatcaat tataagatta tatactatcc ttatactcta gttaaatata ttttaatcac
      121 taatataaat tagttttttt aacaaaacaa taaaagagta aagtacaaat aattcctttg
      181 tactctactc tttttaactt tctttgtaca ataatacaat gataaatata aaaaattata
      241 tattccattc tatagaaata tgctcaccaa taacatgtat gttgttaatc aatatattat
      301 atcaaattat atcaaaatta cataaacctt ataggacaat catatcttat tacaagaaaa
      361 atgtcttatc catattgaat aagtcattca gttcatatac aaagtcaatt tcattgagaa
      421 acatttaata tatgtttata aaaatttcat actattcttt catatttaca tgtgatataa
      481 tttattaact cataattttg ttaatcagtt gtgcttgtat attatttata taatctaata
      541 tttttagaag gtggtgtaat aactttgaaa ttaaaaattg ttggtagtgg cggtatgtca
      601 attattccaa gttcattttg taaatgcaaa atatgtgaag aagccaggca aaaaggcgga
      661 agatatgaac gattaggacc aagtttatat attgatgata taaaaatgct gatagacaca
      721 cctgaagaca ttgcagtagc ttgtaacaga caaaaaatat cggaagtaaa acatatttca
      781 atttctcacc atgacccaga ccatgttaag ggtattagaa ttgtcgaaaa aataggatgt
      841 gactttatca caggagaaaa taatccaatt ggcttttatg cgcttcctga agtaattgaa
      901 gatataaacc gaacgaatct cgattgtctt aaatattatg gtgatgttat aaattgcatt
      961 tctattaatg aaacttcgca tgtaaagata aataattttg atattgattt gattaacaat
     1021 aacccagaca ggaatataac cttctatgtt atcaaagaga atgaaaaaaa agtcatatat
     1081 gcctgctgta atcccaagcc atttactcat agtgatatgt actttgatgc tgatatttta
     1141 ataataagtc tagtttctga tgatggtata ttaaatgatg gtacaaaact taaagacaca
     1201 ccatttaaag atgaaatatt tactttagat gagattgttg aaattaaaaa ctattataga
     1261 attaaaaaaa ttattgttac acatattgac gaaatgtggg gaaaatcata tggttattac
     1321 agtgaattag aaaagaaact tgataacatt tattttgcat atgatggtat ggaaattata
     1381 gtctaaatca ttaagtaata agcataatgc aaaataagaa aacttcaatt taacagtata
     1441 gtcaaactat gtgatatcaa catatcgcag tttttacaaa atatcatagt atttatcttt
     1501 ataaaatttc aagtcctaca tattttaata cttcaatatc taaataaagt aaaatcaaaa
     1561 atatatttta tatacattat agacggctta tcgtcactag ataagccgtt ttgtcacaat
     1621 tattatttgc tcaacaaaac ttctcataat atataacaga ttgaatctcc aactgatact
     1681 taattaccaa aactcttatt ccttaatatg ttgttgcatt aaccaaacct tcatcatcat
     1741 tttttgtggc aatagttttg ctaataaatg actggatttt gtatttatac catacacaga
     1801 aatatcctta tttttctttg catccttcaa tgcttttttg gcaacaacgt ctggagacac
     1861 tatgcctggg aaattttttg tccctttttc agctacaact atacctctct taaatagatt
     1921 tgtactcatc caacctggac atacagcaat tgcatttatc cctttactct taagttcaac
     1981 atttaatgct cttgtataat ttcttataaa cgctttagtt gaactataaa tattttgata
     2041 tggcaatggc tggaatgatg cttgtgaaga aatattcatg atatggcttc ccttttgcat
     2101 atatggaata catataagtc ccaatgctac aacagcacta atatttaaat caatcatatt
     2161 aatagattca tctatactta aatcattgta tgaacagaat tttgcaaaac cagcattgtt
     2221 tataaggtat tttatacata cattttcttt acttaaaaat gtaccaattt cttttacttc
     2281 ttctattttt gataaatctt ttgaaaaaat ttttacctta cttccaaatt catctactaa
     2341 ttgatttaat ctcgccttgt ttcttgcaac tgcccatatt tctgttattt cttcttcatt
     2401 tattaataac tttagaaact ctttaccaaa accactatta gcacccgtta taattgcaat
     2461 cttcttataa ctcataatta ttctccttta ccataagttt atacgctcaa actgtaaact
     2521 tattttttac aaataaaaca ccctctattc agatgttata ttcatattat ttgagatatt
     2581 atatttatat cattttcaat aattgttata tagcataaaa aatagtgtta atattataga
     2641 atcggtattg attata
//
LOCUS       DAYASV010000247         2604 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYASV010000247 DAYASV010000000
VERSION     DAYASV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.252"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(136..2163)
                     /gene="bclA1"
                     /locus_tag="ACGI68_003732"
     CDS             complement(136..2163)
                     /gene="bclA1"
                     /locus_tag="ACGI68_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HFH0140132.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPVGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGNTGVTGSIGPTGVTGPTGNTGEIGPTGATGPTGVTGSIGPTGATGPTGEI
                     GPTGATGATGVTGSIGPTGATGPTGATGVTGEIGPTGEIGPTGATGPTGVTGSIGPTG
                     ATGEIGPTGATGPTGVTGSIGPTGATGPTGATGEIGPTGATGPTGNTGVTGEIGPTGA
                     TGPTGNTGVTGEIGPTGATGPTGVTGEIGPTGNTGATGSIGPTGVTGPTGATGSIGPT
                     GATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNNGDAITGWQTIIGNSSSIT
                     VDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFPILGGKDLAGLTTEPGGGG
                     VLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATLTIFRIADTVMT"
BASE COUNT          630 a          563 c          385 g         1026 t
ORIGIN      
        1 tttaaaattt ttatgaatat attttattat tattgttatt ttattcattc taaaataggt
       61 aaaactcaat atataaaaat acttttgtca tttaaataaa gcctaaatag tttttaagat
      121 tattttagac acgttttaag tcataacagt atcagctatt ctaaatatcg tcaaagtagc
      181 cgcagttcct gctgattgcc catttcgtat ccctactgtt gtagatgaaa aattatttat
      241 tgtaaaagta gtcccaccaa ataaaaaacc agcaaaatat ccagaaagta ctcctccgcc
      301 tcctggctca gtagtaagcc ctgccaaatc ttttcctcct aaaattggga ataggatagc
      361 gaaagaatat tgatttatac ttgatgaacc tggttgtaat gctactgaaa ctgatatata
      421 atacacacca ttttcttgta ctgtaaacgt accatttgca tctactgtta tacttgaact
      481 atttcctatt attgtttgcc aacctgttat agcatctcca ttatttagtg tttgtggaga
      541 tggtgcattt acgaggaagt tagcaactgg ctgagaggaa ttgcctgttg ctcctgttgg
      601 acctgttggg cctgttactc ctgtcgctcc tgttgctccc gttggtccta tacttcctgt
      661 cgctcctgtt ggacctgtta ctcccgttgg ccctatactt cctgtcgctc ctgtatttcc
      721 tgttggccct atttctcctg tcactcctgt tgggcctgtt gctcccgttg gtcctatttc
      781 tcctgtcact cctgtatttc ctgtcggacc cgttgctccc gttggtccta tttctcctgt
      841 tactcctgta tttcctgttg gacctgttgc tcccgttggt cctatttctc ctgtcgctcc
      901 tgttgggcct gttgctcccg ttggtcctat acttcctgtc actcctgttg ggcctgttgc
      961 tcctgttggt cctatttctc ctgtcgctcc cgttggtcct atacttcctg tcactcctgt
     1021 tgggcctgtt gctcccgttg gtcctatttc tcctgttggc cctatttctc ctgtcactcc
     1081 tgtcgctcct gttggacctg ttgctcctgt tggtcctata cttcctgtca ctcctgtcgc
     1141 tcctgttgct cccgttggtc ctatttctcc tgttggtcct gttgctcctg ttggtcctat
     1201 acttcctgtc actcctgttg gacctgttgc tcccgttggt cctatttctc ctgtatttcc
     1261 tgttgggcct gttactcctg ttggtcctat acttcctgtc actcccgtat ttcctgtcgg
     1321 gcctgttgct cccgttggtc ctatacttcc tgtcactccc gtatttcctg tcggacctgt
     1381 tactcctgtt ggtcctatac ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc
     1441 cgttggccct atacttcctg ttgctcctgt atttccggtt gggcctgttg gtcctatgct
     1501 tcctgtcgct cctgtacttc cggttgggcc tgttactccc gttggtcctg tacttcctgt
     1561 cgctcctctt ggtcccattg gacctgtaat tctaattgga agacactcac atttaggata
     1621 acaattatct ataattctat cttcaaatgt tacaagtata tacggtttgt tacaacctct
     1681 actagatgta aattcaagaa tctgataagg gtaatttaaa gataaagcta acccataatt
     1741 aggatattta ttcattgccc atgcttttat agttcccgta atatttaaac aaatatagtt
     1801 ggatgtatca cttatcccaa ctggtaaata tcccctaata ttttccatag acactctatc
     1861 attccatgtt atagttcgta aattataata ttcacttatt cttttaattt caatatctac
     1921 tttttcttta aaaatatgat tcttatttga atcaatatga ataaataact ctgcttttaa
     1981 tatacttttg tcttttaata tatctggtat tctcacttta aaaaaagtaa tcaacttttc
     2041 ttttacaaaa ctatttgaac atttacttcc tattaagcaa taatctaaat tactaaagtt
     2101 tttatctgga tattttttag atataaaagt atcatcattt aaataaagta taatatttct
     2161 catttataat ccctccattt tgggtctata tcatactatg tttaaaatac atgcttagtt
     2221 acaatataat taaaatttat agtattaaat ttttatataa taatgctgat ttctacaaaa
     2281 aaagaacatg tcattagaaa tttctaatag catgttcttt ttcattatta tatattacac
     2341 tttaaattat tccttgactg gcttagctgg tgtaaactaa accagtcttt ctaaaatatt
     2401 aagcactttt ttcttattat gttttttagg atttattgtc tatattattg taaaccaaag
     2461 ttataaaatt gtaaatagaa tatattacca taacaatatc tttttataag taaaaattat
     2521 aaaaaacctt atttttatta aatagaataa ataaaataag tttaaaaaat actgaaataa
     2581 gtgttttcca catattttat atat
//
LOCUS       DAYASV010000248         2552 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYASV010000248 DAYASV010000000
VERSION     DAYASV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.253"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(134..481)
                     /locus_tag="ACGI68_003733"
     CDS             complement(134..481)
                     /locus_tag="ACGI68_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HFH0140133.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(468..2396)
                     /locus_tag="ACGI68_003734"
     CDS             complement(468..2396)
                     /locus_tag="ACGI68_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFH0140134.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
BASE COUNT          906 a          487 c          299 g          860 t
ORIGIN      
        1 tttatgagaa atcaaatata ttttcatcat ttcaacaata caaaagaggc ttatctctat
       61 tttgagacag cctctttcat aattaccttc taatttatat gctctaatat atctataata
      121 attcttatta tatctatata tccagtcttt caatatagtg acaattaact tcaatatgat
      181 ttaaaaatac taacaacctt tcataaagtg gatgagattc ttctccatat ttagcttcta
      241 ggttcttgcc tatttctcta acagtccttt gaccatcaat ttgtaaaaaa acaaaactgg
      301 aatattcatc tagttctatt tttttgtatt taggtattct agcttttaac tttctaaaaa
      361 acttttgaat tttatggtcc tgtttttcaa gtatagtaac aacacgattt tcagctactt
      421 catattcaag tccatcagta attttaaata caatatctaa aacatcatta ctcttgctca
      481 ttatttttta ctttagataa aacaataggt aatgctgtaa gcacaactaa aactactaat
      541 aaagcaattg ccattgaatt tgttgcagca aatccacttg gtacctttgg agttacaact
      601 ccagttactt gtaatattat acctattaaa ccaatgatag aaccaccagc aacaagacca
      661 gaagataaac ttataccatt agaaacttta acttcttttt ctttttcagt tttagatact
      721 aattcaacaa ataaacgaat taatgctcca actaatatta tagaagtagt tgcaattggt
      781 aaatagaatc ctatagctat agtcattatt ggtaaattta atgcatatag aactattgcc
      841 ataaatactc caacaataat cataacccaa ggtaaactac ctgacataat accagaagtt
      901 aatgttgaca ttaagtttgc ttgtggtaat gcaaattgta cattatcacc agttgctcta
      961 agttggtcag aaagtaataa aattactcct acaacaacta taacaccaac tatacttgca
     1021 attgtaaagt aacgttgcat ttcgtttttg ctaccaccaa taatataagt tactttttga
     1081 gattgagtat aacctccagc tatagcaata gcaacaacta taaatgaacc aaataaaagt
     1141 aatgatttat tagcttctaa atcagtccat cccattataa caaacactaa tgttacaata
     1201 actagagaag ctatagtcat accagaaact ggaagatttg atgttccgat tgttcctgtt
     1261 aaacgaccag ctactatgac aaataataaa gataataaaa gagatatgat agctccaata
     1321 attgccataa caatgttacc agaaattaag aatgctgcta aaaatccaat aactactcca
     1381 cctaataaaa gtatcatttg tatagatgaa ccttcttctc cttctccagc acttgattta
     1441 gctttaagtg tttcttttat agaagcgatt atagttggta taagttttat tgcaccaata
     1501 atacctccac aaagcatcat accagcacct atatatttta cataactact agatattgct
     1561 ccaacatcca tttggttaag aggcattgca ggattattcc ataccatagc tccatcttta
     1621 gccatatctg taaagtatcc tattaaaggt gctataccaa agtttgataa aatacttcct
     1681 gcaaacattg ttaaagaaac ttcaagccct actataaatc caattcctaa aagtagagga
     1741 tttacttcaa tatcaaattt ccatttgtag aatttgcttc ctacaagact cattacattg
     1801 tttgctacat ttaagaatga accagttaat acagtaataa atccacttat tacaaatcca
     1861 attcccatgt acttaattga atctccacca gcctctgaag caacaagtgt ctcagatata
     1921 gccattgatt ctgggtacat aagtttacca tgttcttcaa ctattagata gttgtgaact
     1981 atagcagcaa ttcctatacc aaataaaact ccaccaactc caacagctaa gccttctaag
     2041 aatgtaattt ggcttccaat taatataact gctggtaata cgaaaataaa tccacttgca
     2101 acagattctc caccacttga cataccttgg attaagtttt taccaagaat accctttgaa
     2161 cgagcaaatg ctccaacaaa agcagaacct attatagctc ctggaatacc tgcggcaact
     2221 gtaagaccgg ctttcattcc agagtaagtt gtagatgcgg caaaaatggc agctaaaata
     2281 ataccgataa tcagtacagc tacatttcca ccggttctgg acttatctgt gatatatgga
     2341 acgtaatctt ttccactaac ctcaccataa gcacccttag gtaatttttt gttcataaaa
     2401 ttttcacccc ttttcaattt gtatatatta taacatatat acatataaaa cttaactttt
     2461 tattatttta caaatagcga gtcaaaacta ctaaaattta accctatatt attaatttct
     2521 acttttagat atttttatgc taattttctt tt
//
LOCUS       DAYASV010000249         2476 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYASV010000249 DAYASV010000000
VERSION     DAYASV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.254"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            248..2260
                     /locus_tag="ACGI68_003735"
     CDS             248..2260
                     /locus_tag="ACGI68_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFH0140135.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1093 a          234 c          350 g          799 t
ORIGIN      
        1 tttttgggtg tatattataa tttaatcgaa tagaaatgaa aaatatacat acagtttaag
       61 ttttaagcaa caattagggg gatttggtga caagattttt aattataaga aagaaagtgt
      121 cttaatgcaa cttttataaa gtcattatag acactttctt ttttatttga taaatttaaa
      181 ttatcaaaat tatattgctt tatacaacta ttttgttgga aactatttat tatataagga
      241 gaaataaatg aaaaaatcag tagctctggt aaatgatagt agaaaagatt taatagactt
      301 tttagaaaat aatttgaaat tagtttttgg agattctata aatattaata gatacttcat
      361 aaatgaaata aatgataatg atattataaa tgatgatgtg atattagtaa tgtcagtaga
      421 aagattagac aaaattataa ataatatact agataagaaa aaagtgattg tggtaagaag
      481 aacttttagg gaagataaaa tttataatct actatcttta ccacagggta caaatgttct
      541 aatcgtaaat gattctgatg aaactactct agaaactata agtttatttt ataaaattgg
      601 agtgactaat attagaccaa ttccatatat gaatgataat aactataaaa atataaaaat
      661 agctataact ccaggagttc cagaaaaagt acctagcttt atctcagata tatttgattt
      721 aggtcacaga tatatagata tttctacatt tatagaaata ataaatttat tacaaattga
      781 ttcaaaggaa atacaatcta atctagttaa atactcagaa gaaataataa gtttagatac
      841 tggtataaaa gataaatata aggaattatt tttaaaaata gaagaattag atacaatatt
      901 gaatttatca aaagatggta tattatttac ttcaaaagat ggtgaaataa atacatataa
      961 tagtaaagta aaagatattt tagacataaa tgaggatata tatggaaaat acattgagga
     1021 tatatttgta gatagtttaa aagttttatt gagtgagaaa gagatactag ataaagttgt
     1081 cgtattcaat aagaaatata taaatgtaaa taaaaaaaac atatacaata gagatgaaaa
     1141 aatgggcaca tattatagtt tacaagaaat aacatacata aaaaaattag aacaaaatct
     1201 cactaaaaaa ttaagagaaa aaggacaaat agcaaaatat acatttaagg atataaaaac
     1261 aaatagtcct aaaatgtttg aatgtataga tttagctaag aaggtttcta aatctgactt
     1321 aagcatactt atcagaggtg aaagtggaac tggtaaagaa ctcatagctc agtctataca
     1381 taacaactca aatagaaaaa atcaaccctt tatagcagta aattgtgcag ccgttcctga
     1441 aaatttatta gaaagccagc tattcggata tgacaaggga acttttactg gaggtctaaa
     1501 agatggaaaa caaggattat ttgaacttgc taataatgga acaatattct tagatgaaat
     1561 aggcgatatg cctttagaat tacaaacaaa actacttaga gttcttcaag aaaagcagat
     1621 aatgcctgtt gggtctcaca atgtaattaa tatagatgtt agaatcatat ctgctacaaa
     1681 taagaattta gaacaaatga ttgataatag ccaatttaga gaggatttat attatcgatt
     1741 aaatacaatt ccaataaata tacctcctct aagagaaaga aaagaagaca tattaattat
     1801 tatggaagat ttaattaata agaaattagt aatcacacca gaagcaaaaa aacttataca
     1861 aaactatatg tggaaaggta atataagaga actccaaaat gttacatcct atttaaacat
     1921 tatgtgtgaa gatattgtat tagagaaaga tttacctcca aacttaaggt cttcagataa
     1981 caaaaataca tcattaaaac taaaatatag taagaatgat atattgaata tattagagat
     2041 actaatttta aataaggaat cagatgttgg tattggtaga ggattaattt taaaagcact
     2101 actcgataag aaccttcaaa ttacagaagg aaaaattaaa aaaatatttg aatatctaaa
     2161 aaaagaagaa cttataattt gtagttcagg aagatatgga agtaaaataa ctcaaaaagg
     2221 agaagatttt tacaataaat taaaatataa aggtctataa aattataatt tatcaaacaa
     2281 agaaatctat tcaggattaa agtactcctt caataggttt ttttattttt caagtaccct
     2341 gaaattataa tttattttgt acataaataa taggaattaa taggaatagg ataatttttt
     2401 tatttcctat tttttttata taaactagca tataaaatat attttcttca aaataatatt
     2461 ttttactgaa aattga
//
LOCUS       DAYASV010000250         2452 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYASV010000250 DAYASV010000000
VERSION     DAYASV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.255"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(226..1476)
                     /locus_tag="ACGI68_003736"
     CDS             complement(226..1476)
                     /locus_tag="ACGI68_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HFH0140136.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1508..2449)
                     /locus_tag="ACGI68_003737"
     CDS             complement(1508..2449)
                     /locus_tag="ACGI68_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFH0140137.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          734 a          478 c          238 g         1002 t
ORIGIN      
        1 aatacacatt taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat
       61 tatagcatag aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac
      121 tattattaag aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa
      181 acaagggcta aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc
      241 atgtaagaat agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat
      301 cttatttgca tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc
      361 tactttcata tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat
      421 aaaatcaatt cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata
      481 gtcttgtaat atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt
      541 atttgctttc attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc
      601 aggtttatat ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt
      661 tataaattct tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac
      721 cattaaattc tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt
      781 ataatttgga tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg
      841 atgaccatct gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat
      901 tatatcatca ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc
      961 atataaaggt tcattattat ccataaaatc attgattata tcatctatca ccctttgatg
     1021 tttgtaagtt acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct
     1081 atcaatttcc ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc
     1141 atcaactgat atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata
     1201 tatatatata cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa
     1261 attttctcgc gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc
     1321 aatttctgat ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc
     1381 ttctttacta ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt
     1441 tggtctaatc gccttaaatg gtttaatcgt tgccattact aatttcctcc taattataca
     1501 aaatatttta taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca
     1561 catttactat ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag
     1621 gtgtaggaga aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat
     1681 aaacatctat agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt
     1741 catcaactaa accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta
     1801 tttctttttc agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga
     1861 aatctgcatt ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg
     1921 cctcacccat tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa
     1981 cttcttttgc aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac
     2041 ctttatactt tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga
     2101 atcttgcaag aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat
     2161 ttggagtatt cataactttt atgccattag cttgagcata tttaacatct atattatcca
     2221 gacctactcc acctcttact atcaacttca actttttacc ctctaatgct ttatctatta
     2281 catctttagt aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat
     2341 ctcctaaaac atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt
     2401 ttctcgctgc ttctttctct attccatcag taactaatat attatacata ac
//
LOCUS       DAYASV010000251         2434 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYASV010000251 DAYASV010000000
VERSION     DAYASV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.256"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            279..>2434
                     /locus_tag="ACGI68_003738"
     CDS             279..>2434
                     /locus_tag="ACGI68_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HFH0140138.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTSKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSSSGGSSSNQTSVNNEAAKITSVPTPAKDATRLTMPSVSSGYTIAIKTSSNE
                     SVIKKDGTIIPPNTATTVKLVFTVTHTSSGKTADTKELSVTVPAKSTDEELQAALDNE
                     VAKITSVPAPAKDATKLTMPSVSSGYTIAIKTSSNKSVIKEDGTIIPPNTEETVTLVF
                     TVTQESSGKTADTGEIDVVVPAKSTDGEIQAEVQAEA"
BASE COUNT         1055 a          328 c          435 g          616 t
ORIGIN      
        1 tacttttttt atacaaaacc ctatttttta taacaattca ttaacataaa aatcaaactt
       61 accttaaaat ataaatttac aaatttttca atcaataaga aaatttcttt attccttaca
      121 taatgactgc aatgtaacaa aataattaca aattaattat gaaatcatta tagttagaaa
      181 cctctttaaa attagatatt aagtatcctt tagaattaga acgggaacaa agttaaaaaa
      241 ttaagtaata tcaaatggac aagggggaag aaaataaaat gaaaatacca aagaagattg
      301 ctgcaatgct tacagttaca atgatagctg gaagttcaac agcaggcata gctagtgcac
      361 aaactgtggc aacaaattta acagggcaag aaagatatga aactgctgtt aaaataagtc
      421 aagatggatg gaaaaatgct gatgaggtag taatcgtaaa cgactcatct atagcagatg
      481 ctctatctgc aacaccattt gcaaaagcaa aaaatgctcc aatactttta acaagtaaag
      541 ataaattaaa tgataagacg aaagcagaaa tacaaagatt aaaagctaaa aaagtatact
      601 taataggtgg aacttctgta ttaagtacaa atatagaaaa agaaataaaa gatttaaaaa
      661 tatcttttga gagaatctct ggagctgaga gatatcaaac atccttagaa ttagcaaaga
      721 aattagatgc tataagtgat gttaagaaaa tagctgtagt taatggtgaa aaaggacttg
      781 cagatgctgt aagtgtagga gcaccagctg cacaaaacaa tatgccagta atacttgctg
      841 attctaaaaa tggaacagca gttgcagaca aatttataaa ggatgctgga ataactcaat
      901 cttatgtagt aggtggagag agttcaattt ctgaagctgt taaaaataaa ctaccaaata
      961 gtacaagact aggtggaaca gatagaaatg atacaaatgc aaaagtaata aaagaatttt
     1021 ataaaaagac agatttaaaa aatgcttatg taactaaaga tggtatgaat aaacaagacc
     1081 aacttataga tgcactagca gtaggtgtat taggtgctaa aaatcaatca ccagtagttt
     1141 tagttggaaa gaacttatca gcttcacaaa aatctctagt taactctaag agttttgata
     1201 agataactaa agttggtggg aatggtaatg agacagcatt taatgaaatg aagtcattac
     1261 aagaagtaaa aacagttgaa gctaaaacaa tttctgaatt aaaatcagct atagataaag
     1321 ctactgctaa tgatgttata aactttaagc caactagtga ggttaaagaa gcatttacaa
     1381 tacaaactga taaagcagta acagtaaact taaatggtac atatacaaag actgtgacta
     1441 tcaacatgcc taatggtgat gtaaataact atgcaaaagt ggatgatgta gttatagatg
     1501 atgtaaaaga tggaactttt gttaattatg gaaaaataac taacttaaag gttaatgata
     1561 aaaatggagc caagatagaa aataattcta aaggtgagat tggtagttta acagtagcat
     1621 ctggagcaag tcaagtaaaa gtgactaatg gtggtaagat aactacagtt acaaataatt
     1681 ctaaagggac tactatagat aataaaggta caataagtag tgttaaggga gataattctc
     1741 caacaataag tggaaatagt ccaagttcta actcttcagg aggaagttca tcaagtggag
     1801 gttcttcaca tggaggaggc tcatcttcag gaggaagttc ttctaatcaa acatcagtaa
     1861 ataatgaagc agcaaaaata acaagcgtgc ctacaccagc aaaagatgca actagattaa
     1921 ctatgccatc agtatcaagt ggatatacta tagcaataaa aacatcaagt aatgagagtg
     1981 ttataaaaaa agatggaaca ataatacctc caaatacagc aacaacagta aaattagtat
     2041 ttacggtaac acatacatca agtggtaaaa cagcagatac aaaagaatta tctgtaacag
     2101 taccagctaa atcaacagat gaagaattac aagcagcatt agacaatgaa gtggcaaaaa
     2161 taacaagcgt tcctgcacca gcaaaagatg caactaaatt aactatgcca tcagtatcaa
     2221 gtggatatac tatagcaata aaaacatcaa gcaataagag tgttataaag gaagatggaa
     2281 caataatacc tccaaataca gaggaaacag taacattggt atttacagta acacaagaat
     2341 caagtggtaa aacagcagat acaggagaaa tagatgtagt agtaccagcc aaatcaacag
     2401 atggggaaat acaagcagaa gtacaagctg aggc
//
LOCUS       DAYASV010000252         2414 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYASV010000252 DAYASV010000000
VERSION     DAYASV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.257"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            93..566
                     /locus_tag="ACGI68_003739"
     CDS             93..566
                     /locus_tag="ACGI68_003739"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0140139.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(935..1492)
                     /locus_tag="ACGI68_003740"
     CDS             complement(935..1492)
                     /locus_tag="ACGI68_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0140140.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKATIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLL
                     SKEEAIKGLADSIDTVLLGIHKKYD"
     gene            1750..>2414
                     /locus_tag="ACGI68_003741"
     CDS             1750..>2414
                     /locus_tag="ACGI68_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0140141.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIIENGKHWFNTPQ
                     ELNS"
BASE COUNT          890 a          320 c          299 g          905 t
ORIGIN      
        1 aatattctaa atttataaaa caaattgtat tgaattgaat ttatttaaga tatataatta
       61 taattaaaat aataataaag gtgttgttta taatgttcaa agaatactta ataagagaat
      121 taaaagaaga tgatattaaa aatgcattag acttaatctg gaacgtattt gaagaatttg
      181 aatctccaga ttattgtgat gaaggaatta cgacatttca tcaatttata gaatataact
      241 ctattattga aaaaatcaaa gaaaaaactc tcttcttttg gggttgtttt gactctaaaa
      301 aacttattgg agtcttagct attagaaatt taaatcatat atgtatgtta tttgtagata
      361 aaaactatca taaacatggt gttgcaaaaa aattaatgac tacaggcata caatactgta
      421 tttgtaataa atttaattta actgaaataa ctgtaaattc atcaccttat gctgttgaag
      481 tttatcataa attaggattt aaagatactg ctgttgagca aattgttgac ggtatacgtt
      541 ttacacctat gaaattgtta atataataaa ttaaataaaa agcaaagtct cttaaatatt
      601 ttatagttac tttgctttta aaccaagatt taaaataata ctatcttttt tgttttttaa
      661 taatatactt taaaatcctt actaatatga actataattt attttttgat atcctttttg
      721 attaacatat aaattaattc aattattaaa cataaactat gttgtttctc tttatatctc
      781 ttctttgtaa acaatattgg gcatttgaaa ataaatattc ttaaaatata ctgttctcct
      841 gttgattttc ctttggtaca aatgataaaa atttaattta catgccccat aatgaagtgt
      901 tcaataaata agttaaaata atttatttac ctccttaatc atatttttta tgaataccta
      961 ataacacagt atcaatagaa tcggctaaac cttttatagc ttcttcttta ctcaaaagtg
     1021 atttcccaag ttgcaaagtc gaataaccat gaagtacact cattattaag tttaatactt
     1081 cacctgtatt ctttgtattt agattacaag aaagaataag tttttctaat aaggatttat
     1141 attcatcaaa gatttctaat gtttcattat ttccatgcca agtagcccac tgaattgttt
     1201 cataaacacc aggattagaa attataaaat taaagtatgc tatacttata cactttatag
     1261 ctgtatctcc aaaatttcca atagtcgcct ttatcatttg actattcatc tctctcattc
     1321 ctttatgagc tacttcaagt aataaatcat ctagactttc tatatgattg tacaaagatg
     1381 gtgtacgtat tcctagcttt tctgcaataa cttttaaaga aactttgtta agcccatctt
     1441 tattaactat atctgatgta gcttgtataa ctatttctct actaacacgc atgattttcc
     1501 ctctttcatt taaaattaat tactttatta gtttagctaa tctaattagt ttcgtcaatt
     1561 attgtaattt ataaaattct ttatactatt ataaaatctt gacagtttaa ttataattta
     1621 atataatact aattgaatta gttttaatac taatataatt agtattatat taaattctat
     1681 attacaacat tattttatag tttgattaat ttaacaatca ctaatatatc aaactgaaag
     1741 gagattttta tggaaaaaaa atatttcgaa attcatggag ttccttcaat cctttgggga
     1801 aatccttcaa gtaaattata tatttacata catggacaat gtggcaataa agaggaggct
     1861 gaagcttttg ctaatattgc agttccacat gagtgtcaag ttttaagtgt tgatttgcct
     1921 gaacatggag aacgtaaaag agaaaaaaat tcatttaacc catggcatgt tgttccagaa
     1981 ttagtatcta ttatggaata tgcaaagtgc cactggaatc aaatttcgtt atatgcaaat
     2041 agcattggag catggtttag tatgttaagc tttgaaaaag agaacttaag agagtgtatt
     2101 tttgtatctc caattctaga tatgcaacag ctcatttcaa atatgatgct ttgggcaaat
     2161 gtatcagaag aacagcttaa acatgaacta attatcccaa cttcatttgg gcatactctt
     2221 tcatgggaat atctacaata tgtaaaacaa catcctatca ctcaatggaa tgtaccaaca
     2281 aaaatattat atggtaaaca tgatgaactt actgaaatcg gtgttgtaga gaaatttgta
     2341 gaaaagttta attgcaacct tactattata gaaaatggca aacattggtt taacacacca
     2401 caagaattaa attc
//
LOCUS       DAYASV010000253         2249 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYASV010000253 DAYASV010000000
VERSION     DAYASV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.258"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            115..255
                     /locus_tag="ACGI68_003742"
     CDS             115..255
                     /locus_tag="ACGI68_003742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140142.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(252..623)
                     /locus_tag="ACGI68_003743"
     CDS             complement(252..623)
                     /locus_tag="ACGI68_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0140143.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1172..1429
                     /locus_tag="ACGI68_003744"
     CDS             1172..1429
                     /locus_tag="ACGI68_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140144.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1539..>2249)
                     /locus_tag="ACGI68_003745"
     CDS             complement(1539..>2249)
                     /locus_tag="ACGI68_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFH0140145.1"
                     /translation="KHKFERGQNKMIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNV
                     MIHLVWVYIFLLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNI
                     IMFMPLGFLLPLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAV
                     LGYFIWVGVNNLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYL
                     ILAVLGEFLLFNWSIIPY"
BASE COUNT          910 a          255 c          257 g          827 t
ORIGIN      
        1 gataatcaaa atatatttta taaaatcaaa atacaattac ataaacttta ttaaaaattt
       61 gttgggaatt tctgactaac attaagaacg taagtttgtt gggggatggt atatgtggaa
      121 aagtcaaaca ctactgttac aaaaaaagaa aaaattgaag atataggaat gaaattagaa
      181 catttattga gtgaagatat tactttttat gaattattac ttgagatttt gtataaagaa
      241 aaagaagaga attaattctc ttctttaagg aatcttttta aataggcatt atcaatcatt
      301 ttttctatag cttctctatc ttctttttca agagaatata ttttttctaa gagctttttt
      361 gttttttcac ttactgataa gttctctatg attaatttat cttctttgct tatttttgta
      421 ttatcattta cgcctaataa ataatctgtt gatgtttcta atgcttttgc taatctaact
      481 attatttcac ttttaggttc tcttaaatta ttttcataac gtgacaagct agcctcagtt
      541 ataccgactt tagtagcaag ttctttttga tttatatcta attcttttcg tagatttgct
      601 attctatctc ctaaactttc catatgtaca tcactcctta ccgaaattat aagttatttt
      661 gataaaaagt aaaataaaat taccaaaaat ataaaaaaaa aaggttaaaa taataaaata
      721 tattgaaaat taccatttgt atatgtataa tatgaattat acaaatggta atttgatgag
      781 aggtggtatg ttgaaataag aaactgaatt aattaataaa tacatagaaa gttttgaaaa
      841 cctttaattg agatataaac aatatgtaca aaattttttg caagtaaaat taccaaatgt
      901 ataaaataaa tcagtagcat agataatttt atgttttaat acattagatt atttaattta
      961 gttataatgg tcaaaaatgc aatctacaaa taaaacaaag tattatcaaa aaagtattct
     1021 ttaagaaaat tttttattag agatgtaatt gatataaata atcgtattaa aaaataaaat
     1081 actaagttta aaaatttaaa taatctataa atcaattata aaatattatt tattagaaat
     1141 taaaatgaat taagtaaatg gaggaattga tatgataata attagaagtc aagataaaac
     1201 taatttaatg cagataaacc aaattaaaat agatggtagt caagtttatg cagtatttga
     1261 aagtaaaatt gatactgtaa aaataggaga ttatgaaaat aatacaagag ctatacaagt
     1321 gttggacaat atacaaaact tcattgaaaa tggaacaaaa tatgattata taacatcaaa
     1381 taaagtcaga tacaatgtta ataaaatatt tcagatgcca gctaaatagt gagtgtgcat
     1441 tttaatttaa taactaaagc ccactttctt ttgtagtatg aaagagggct ttacatattt
     1501 gtaaatatcg aagtacactt attttatcaa taaattattt aataaggtat aatggaccaa
     1561 ttaaacaata aaaattctcc taaaacagca agtattaaat atataatagc ttcatttttg
     1621 taaagagagg taattttttt gcttatgtca tcatcaattg taatagcaat ctcatcttga
     1681 ataatatttt tatatataga tgggaaaata tcttttttct ttataagatt aaataaatta
     1741 tttacaccta cccatataaa atatcctaaa acagcaccta atgtgttcat cattaaatca
     1801 tcaatatctg tagctcttaa gttaaacaat tgacaaagtt caattgctaa tgaaaaacct
     1861 aatccagtta atgaaacatt gataatattt ctaaaatttt tccaaattaa aggtagtaaa
     1921 aatcctaaag gcataaacat aattatattt aaaatatatg ttaagatgcc aactgagtca
     1981 aaaggtatta gatttatttc atcaattcta aaaagcccac tataccttcc tatatcccat
     2041 atgctaccaa ttccagcttt actaagtgct agatagatat ataataaaaa aatatatacc
     2101 catactaaat gtatcattac attcttttca actttcaaat tttttcgttt tataacgact
     2161 atttggtata ttaagcatgg taatataacg caaataaatg aataaaatgt ccatatcatt
     2221 ttattttgtc ccctttcaaa tttatgttt
//
LOCUS       DAYASV010000254         2245 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYASV010000254 DAYASV010000000
VERSION     DAYASV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.259"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(94..1008)
                     /locus_tag="ACGI68_003746"
     CDS             complement(94..1008)
                     /locus_tag="ACGI68_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HFH0140146.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRSYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1835..2017)
                     /locus_tag="ACGI68_003747"
     CDS             complement(1835..2017)
                     /locus_tag="ACGI68_003747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140147.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          820 a          280 c          283 g          862 t
ORIGIN      
        1 taaattttat aagtttataa aaaataaagg tacagttaac tgtaccttta ttttatctaa
       61 atatgcaaag attattttaa aagtaatttt taattacatg tttttataac tctccaatat
      121 tctaatataa tgatttgcct ctcttaaaac atggtctgct aaaagtggta atattataga
      181 ttttatctta cactgttcta ttcctgatgc tcctgcttct ttaaaatctt taaactcaac
      241 agtttcattt agagtttctt ctgtaatgtt cttgatatta gaatcagtca tttcgtttgt
      301 tttttcaatt aattcattaa attttatagc aaaatcattt gaagtattta ttagttcacc
      361 ttctgagggg tctagtaatc ctctcataaa cagagcatgt tccatcataa tatggtccca
      421 gaataattct atttctttag cgttttttga ctcaatatct attttatttt caaggtcaac
      481 tacataagaa cgatataaat ttgcttcatg tattatatgt tcaagaagta gagggtagtt
      541 tgatgtaaat atagtacatg atagtacacc atctaagact ctttctttaa aattaataag
      601 cccatcaagt aatcttattg catcagagtt aagttgagct acatagttca ctaagtcttg
      661 accaacttgt gggtttacac cactttgtag atttaattct cttgtagtga tgtttttgtt
      721 tatttctatc cctgtaaact cttctgtttt ttgttctgca actgatgtga gagtagttac
      781 aagctcttct gaatataata tatcaggtct aattatacca ttactagcac taacagtgta
      841 tgataataaa tcttcaaatt gctttttata gtggtcagct tccatagcaa gattataatt
      901 tttatttgtg aatcctgctt ctaaaaaaag agcatgctct ttcataattc ttgaaaaaaa
      961 taaatgtaat tctagtgata aaataacata tttttgatta tctatcataa tttcctcccc
     1021 ttagaaagta tcctatttat aatactttat tcgtaatatt taaatttatg tatattataa
     1081 ataaattaac taaattcaat tgctactaat atcaacagtt tttattaagt tatgggcgaa
     1141 aagattatct tactgtgttt cttattagat atagatataa tgatttaagg tacttgttgg
     1201 ctattattta ttaagagtac aagtaatact agaaattttt atgagaatta tgtaatttat
     1261 aagtattatt ttgatacaaa acaaatgttt tttgtaaaac aagaactttt atagcaaaaa
     1321 atgaataaat atgaagcaag cataatttta taccacaaca attattaatt ctaatagctg
     1381 cacttaatgt tttaggtatg ggatttaaaa agtataaaaa attaagtgat aaatatattc
     1441 cattatattg cttgtgcttg gagtagtatt ttctgtatag atgttaggat ttaatccaac
     1501 ttctatttta caggggattc tatgctgggg tgtttcaatt ggattaaatt aagtatgcaa
     1561 acaatttcaa gacaatgata aataattgga tgtgtgatag gaatatcaac aaaaaacatt
     1621 attaaaaaga attattttat aaataaaaca agtaataaga ataaataagg agcaactaaa
     1681 atgatttaaa tatactagaa ttaaaaagag aatgtaaagt tgtctagtaa tggatgtaaa
     1741 atattttaaa cgataagcta tgaaattgat atattaaaat gggtaagaag aatattatat
     1801 aggattcttc ttacccattt taataaattt aattttatgc caaaccacat tcgcatagta
     1861 gaatcatcac tctatggaga cataactcta caacatcttc tccatatttt ttaataaaat
     1921 caccaatttg agtttctatg aatgaatagt attcttttaa actagcaata tatgcagtca
     1981 ttatatgaac acattcatca atcttatctt taaacacaac taatccccct aaaaaacgaa
     2041 aaataaaaat attcagtaaa tagcagatat tatttcactc ataaatctat atagtatgat
     2101 atattgctta gatatatttt acaagtggat atgtaaaact ggttattata atcgcaaaac
     2161 tggataaact ttcattctta aaagaaaatt tatccagttt aaagtttaat tatgaatatt
     2221 tgagttagta gatatttaat ataaa
//
LOCUS       DAYASV010000255         2225 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYASV010000255 DAYASV010000000
VERSION     DAYASV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.260"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            475..2004
                     /locus_tag="ACGI68_003748"
     CDS             475..2004
                     /locus_tag="ACGI68_003748"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase domain-containing protein"
                     /protein_id="HFH0140148.1"
                     /translation="MKSLKALIVGVSKYSIVKNKDLLFCKNDIIEINNVLHKRLGITS
                     NNIRTLGMEYEVRKDLFFKTFQEISDSIGDDDTFIFYFSGHGCICNNGVHYLVLSDEL
                     VSIQFVIDFIEGLNCKNKIVFIDCCFAGKFNVSDKQKINIKDIFSLFDRKGCAVLVSS
                     SEYQESFSLPDSSISRFTSLLSEALRNEYIVRKGELSLRAISKYINLRMSILNKNYIN
                     CKQNPIFRSNILGDIYFNVKDYKSYIKKKVFQEHDDYIIYNVKSLHISIIKRYSVEVI
                     LKNKFSYCDISEISKEVFDIVKSVEVYNSEIGEDRLKGKDANIIWVYFGKDEIDMING
                     IYMCKTTWIDKYQEKDIWYSVNGKNKFFINGVHTETYDNYESLKSYISQNTITKEKAV
                     YEVKEILTNMINISEEIISFYNEYENKKICEDNIFQIIDSRKKKMDECYNKVINLDFP
                     PIEIKDWFDKCINLVSTIQNFGVYWDHSRTEENRKQCMEMNIEDYYKDLREVIRLSEE
                     I"
BASE COUNT          961 a          167 c          351 g          746 t
ORIGIN      
        1 gtttttaaag atgtatgtct aactttagaa atgtatgtat aattttataa ataaagcata
       61 acaatttaaa taaatacttg agggaataaa atgatattaa ataagatata aatgattgga
      121 aatatttact cactttaaaa ttcttatgga tagtagaata caaaaaacac atgaaattct
      181 attaacatca atattttggg gaattttaaa aaatatatgg tgaagagata gatgatttta
      241 cattggttta aattacacta agttcaggtc agataaatca gagatataaa ttatatgggt
      301 gcgatgtaaa taaatatata tatacttacc ttgaaaaatt ataatccaac tacaaaagta
      361 aataatgaaa aagtcaaaaa agtagtgtga tactagcttt tataaaatca ataaaacaat
      421 taaaacatca ttttaagtat attaaattga aaaattataa agggggaatg gaatatgaaa
      481 agcttaaaag cattaattgt aggagtaagt aaatatagta tagttaaaaa taaagattta
      541 ctattttgta aaaatgatat aattgaaatt aataatgtgt tacataaaag actggggatt
      601 acttcgaata atataagaac tttaggtatg gagtatgaag tcagaaaaga tttatttttt
      661 aaaacttttc aagaaataag cgattctata ggagatgatg atacatttat attttacttt
      721 tcagggcatg gatgcatatg taataacgga gtgcattact tagtattaag tgatgaatta
      781 gtttctattc aatttgtaat tgattttata gaaggattaa attgtaaaaa taaaattgta
      841 tttattgatt gttgctttgc aggtaaattt aatgtaagtg acaaacaaaa aataaatatt
      901 aaagacatat tttccttgtt tgatagaaaa ggatgtgcag tgttagtatc aagtagcgaa
      961 tatcaagaat cattttctct tccagatagt tcaattagta gatttacaag tttattatca
     1021 gaagcgttaa gaaatgaata catagtaaga aaaggggagt tatctttaag agcaataagc
     1081 aagtatatta atttgcgtat gagtatattg aacaaaaatt atataaactg taaacaaaat
     1141 cctatcttta ggtctaacat actgggagat atatatttta atgtaaaaga ttataaatct
     1201 tatataaaaa agaaagtttt tcaagagcat gacgattaca taatatacaa tgtcaaatct
     1261 ttacatataa gtataataaa aagatattca gtagaagtta tattaaaaaa taaattctcc
     1321 tattgtgata tatcagaaat atctaaggag gtttttgaca tagttaagtc tgttgaagtt
     1381 tataatagtg aaataggaga agatagatta aaaggaaaag atgctaatat aatatgggta
     1441 tattttggca aagatgaaat tgatatgata aatggaatat atatgtgtaa aactacttgg
     1501 attgataaat atcaagagaa agatatatgg tatagtgtaa atgggaaaaa taaatttttt
     1561 atcaacggtg ttcacactga aacatatgat aactacgaaa gcttaaaatc atatataagt
     1621 caaaatacaa tcactaaaga aaaggcagta tacgaagtta aagaaatttt aacaaatatg
     1681 ataaacatat ctgaagaaat tatatccttc tataatgaat atgaaaataa gaaaatatgt
     1741 gaagataata tttttcaaat tatagatagt agaaagaaaa aaatggatga gtgttacaat
     1801 aaagttataa atttagattt tccaccaatt gaaattaaag attggtttga taaatgtata
     1861 aacttagtgt caactataca aaattttgga gtttattggg atcatagtag aactgaggaa
     1921 aatagaaagc aatgtatgga aatgaatata gaggattatt acaaagattt aagagaagtc
     1981 ataagattaa gtgaggagat atagaattaa atttttccat agttgtacta atatagaatt
     2041 ataagtcaga ttggtacttt gttggttacg aatttaaatt aaaataagtt gtttacacta
     2101 aacatgatag aagatacaat tataaatcca tttataaatt atattggtat tgatttaaat
     2161 aattagatat ttattttgaa aaagtctagt aaatatttaa aaacgtataa tatgctgtta
     2221 atgga
//
LOCUS       DAYASV010000256         2209 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.261, whole genome shotgun sequence.
ACCESSION   DAYASV010000256 DAYASV010000000
VERSION     DAYASV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.261"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(123..1415)
                     /gene="brnQ"
                     /locus_tag="ACGI68_003749"
     CDS             complement(123..1415)
                     /gene="brnQ"
                     /locus_tag="ACGI68_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0140149.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          735 a          372 c          246 g          856 t
ORIGIN      
        1 tttatcaaac aggattaata atgttctttt atctacaata atttaataat tcttttaaat
       61 ataaaaagga attgcctata taatttaaag gcaattccat aattatcaat tagtttttat
      121 tattatgcag ttttactgct tttagatgga ataaagtttc ctattattcc tgctattaca
      181 actggaacta tccagttaaa tcctaatgaa gcaaatggta agctattaac tagaggaatt
      241 tttacaccaa aacttgttgc aactgttaat acacttacta ataatgccat gtatgcagca
      301 catttgaaca cgttgtcatt tttaatttta ttactaaata atgctagtat aactagagtt
      361 atagttgctg gatataccat acttagtata ggagaagaga attgtattat tgtacttact
      421 ccaaagtttg atatgattgc actaaataca caaactacta taactatatg ctcatacttt
      481 aattttccat ctgtaagtgt tgagaaatat tgaccagctg ctgaagaaag acctatagct
      541 gttgttaaac atgctaatgc aactataata gccaataaaa ttttacctgt agttccaagt
      601 aaagctgctg ttattgcaac tattaaagct gtttgttgaa catcttgtcc atattgttga
      661 gacacagttg ctccaagata acataatcca ccataaacta atgccattcc tattactgca
      721 attacacctg cacttattgt ggctttcatc ttttctttct cacctgtata acctttagct
      781 attactgaca tgattataat tgatgcaaat acactagctg ccaatgcatc cattgtttga
      841 taaccttgag ttataccttc tgcaaataca ttttgtatta aagaactgtc tcttatttct
      901 cctaatggag atacgatacc tttaactatt aatactgcta aagctataag taaagctgga
      961 gttaatattg acccaactat atctacaact tttgatggtc ttattgttaa aaccaatact
     1021 agagcaaaga atactataga gaatattact gggcttattc cagaacctat tgtaggtaat
     1081 atacccattt cataagttgt tgctgctgtt ctaggtattg ctaaaagtgg ccctatacag
     1141 attaccattg cacatcctaa tgcaatactt aatcctttgc ctgcccttga gaacatcttc
     1201 gatgtgtctc cattgaactt agttgctgct ataactgcta ataatgccaa accaccatct
     1261 gcaaaaagga atcctatgaa agctactagc caactacttc cagataaaac tcctagatat
     1321 ggagggaaga ttaagttccc tgcaccaaaa aacattgcaa atagtgcaaa tccaattaca
     1381 attacatctt taaaattgat ttgtttctca cccatttaaa gcacccccat ttgaatttta
     1441 ctacctttta ctaaaaaata agtccaatct cttaattaaa tgaaaacctt ttgacgagat
     1501 tttatttaat tatacatttt tgacttaaat ttgtcaatta tgtttcataa tttataaaat
     1561 cttcacaata tttaatacaa ctttcttaat taaacaaaat caacctgtat actttaaaca
     1621 tagttggttt tttgtttttt ctctaatttt tcttgataaa attaactatt ccgaataatt
     1681 aaaatattaa gactttttag attattcgat ttattattaa gttgtattgc tttatatact
     1741 gtatatatca ctctcctttt ttacattcat ataatctttt taattcaatt taaattttaa
     1801 atattttgtg tttattatat aatctgaaaa aaaagcagtc aatagcattt ttaatttttt
     1861 taattttcaa aattatgatt cttaaaaaat cgttttcctt ttatcataac acctatttac
     1921 gttaattctt gtagtatttt gtatttattt tgctaaattg acagttaact ttattcaatt
     1981 tttattctac ttgcgtagat ttgcctcttg ttttttactc ttaaaaccat tttttctgaa
     2041 aattccttat tatgatttat caacttattt ttcttcataa caattactct ctaacttcca
     2101 acttacttaa actttgcagt tgctagtatt cacgtttaca tacttatatg tcataattaa
     2161 ctaaaaattc ttaatatatc tctcaatgaa aacaatttac tcatttatt
//
LOCUS       DAYASV010000257         2190 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYASV010000257 DAYASV010000000
VERSION     DAYASV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.262"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            249..380
                     /locus_tag="ACGI68_003750"
     CDS             249..380
                     /locus_tag="ACGI68_003750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140150.1"
                     /translation="MMRLNEKKIMIQLDFLEKKYGTEYLDFLKDYIEFIKKCYNKVS"
     gene            complement(430..822)
                     /locus_tag="ACGI68_003751"
     CDS             complement(430..822)
                     /locus_tag="ACGI68_003751"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140151.1"
                     /translation="MIDGKNTEELNTINKRIKKIRELEGLSQEEFAKKIDLSQNHLSG
                     MENGKKKVMNRTIITICSIFNINKEWLENGIGEIYNPIEENSLNLSDKNQELIDMIYS
                     LDEEDQKVLTLMIENLYNKAKKIREEKE"
     gene            1004..1186
                     /locus_tag="ACGI68_003752"
     CDS             1004..1186
                     /locus_tag="ACGI68_003752"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140152.1"
                     /translation="MNEENKKATTLLQKLNYISSTEDEDILKRKEVIVATVENFCSLD
                     KYNNNLDLKSKQDTIK"
     gene            1394..1603
                     /locus_tag="ACGI68_003753"
     CDS             1394..1603
                     /locus_tag="ACGI68_003753"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0140153.1"
                     /translation="MKKNKLSVLREENGLTKTDVAIFLNMTPSGITGIERSTKISLDK
                     AIKLSEIYGVSIYDIFNASREFMEA"
     regulatory      1608..1692
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1883..>2190
                     /locus_tag="ACGI68_003754"
     CDS             1883..>2190
                     /locus_tag="ACGI68_003754"
                     /inference="COORDINATES: protein motif:HMM:NF017834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140154.1"
                     /translation="MRELKENKIKDLYLKGYRAKEIATTLEIGYESVRKYIVRNCKDL
                     KEIHKKNSNSIIEEIRKLYSEGYNTKEIAHVLHKNIDMIEKNITRNCRDLKKIHKKNN
                     "
BASE COUNT          893 a          215 c          305 g          777 t
ORIGIN      
        1 atatatattt agaaaatact ggaaaatatt aaaaatatca taattctata gatttaaaat
       61 gaaaatatat tgtacgtatg tagaataatt tatattttat aaaatatata aggtaatatg
      121 taaaaaaaac actgaaatat aggtcgtacc aatatttttg aagtaatttt ttgtgctaaa
      181 atgtaaataa gttaaaagat taatttatta aaggttatct tattatagtt aaattggggg
      241 atgatgatat gatgaggttg aatgaaaaaa aaattatgat tcaattagat tttcttgaaa
      301 aaaaatatgg aactgaatat cttgacttct tgaaagacta tattgaattt ataaaaaaat
      361 gttataataa ggtatcttag tgttactatg attaatgtta tataaaaaag agtagataat
      421 ttttaaaatc tactcttttt cttctctaat tttttttgcc ttgttatata aattttcaat
      481 cattaatgtt agaacctttt gatcttcttc atctaaagaa tagatcatat ctattagttc
      541 ttggttttta tcagataaat tcaatgaatt ttcttctatt ggattatata tctctcctat
      601 tccattttct agccattctt tatttatatt aaaaatacta caaatagtta ttatggttct
      661 attcataact tttttcttac cattttccat accactcaga tgattttgag acaggtcaat
      721 ttttttagca aattcttctt gtgaaagtcc ttcaagttct ctaattttct ttatacgttt
      781 atttatagtg ttaagttcct cagtattttt tccatctatc aaaatatcac ctcctaataa
      841 atcttttaaa gatattttaa cataattaga aaataaataa aatctctaag aaatatttta
      901 tctctaaaaa gaaaatttca ccttgtgttt tatctcttaa agatatataa tgatgataaa
      961 gtaaaataat atctttagga gataagagag agaggtgttt ttattgaatg aggaaaataa
     1021 aaaagcaact acccttcttc aaaagttaaa ttatatctct tctacagaag atgaagatat
     1081 attaaagcgt aaagaagtaa tcgttgctac agttgaaaat ttttgttcat tggataagta
     1141 taataacaat cttgatttaa agtcaaaaca agatactatt aagtgacaca agcaaaatga
     1201 ttgtgttatt aaagttatta aataaaaact agaatattta tgtattctag tagatgaaaa
     1261 aagtaaaata tatttattat ctaatgacat agataaagaa attatatatt tatttaacaa
     1321 gttaactttt tcaagttttt ccatgagata tttaattact ctttaaaaaa ataatgaaga
     1381 tggtgatttt taattgaaaa agaataaatt atctgtatta agagaagaaa atggtcttac
     1441 aaaaactgat gttgctatat ttctaaacat gacaccatct ggaatcacag gtattgagag
     1501 gtctacaaaa ataagtttag ataaagctat aaaactatct gaaatatacg gtgtttctat
     1561 ttatgatatc tttaatgcga gtagagaatt tatggaagca taaaagcaga atataggggc
     1621 aaagctagag aaatttagtg acgcaaaact agagggacta aggctaatta gctaagtcag
     1681 ccagttaccg aaaagagatt atctttttgt attttagtat aaaagatgct cgaggtatct
     1741 tttttttagt ttgtaattga attggcaaaa aatatgttaa caaactaaaa atgtcccaaa
     1801 atgtccgaaa tgtccgaaat gtccggaatg taataaaatt caaataactc tatatttatg
     1861 tcttttttta aggaggaaac aagtgaggga actgaaagaa aataaaataa aagatttgta
     1921 tttaaaagga tatagggcaa aagaaatagc tactacatta gaaataggtt atgagagtgt
     1981 gagaaagtat atagttagaa attgtaaaga tttaaaagaa atacataaga agaacagcaa
     2041 ttctattata gaagaaataa gaaaattgta ttcagaagga tataatacaa aagaaatagc
     2101 acatgtttta cacaagaata tagatatgat tgaaaaaaat ataactagaa attgtagaga
     2161 tttaaaaaaa atccataaaa agaacaatga
//
LOCUS       DAYASV010000258         2070 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYASV010000258 DAYASV010000000
VERSION     DAYASV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.263"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            139..1353
                     /gene="dpaL"
                     /locus_tag="ACGI68_003755"
     CDS             139..1353
                     /gene="dpaL"
                     /locus_tag="ACGI68_003755"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFH0140155.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            1480..>2070
                     /locus_tag="ACGI68_003756"
     CDS             1480..>2070
                     /locus_tag="ACGI68_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HFH0140156.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          810 a          220 c          445 g          595 t
ORIGIN      
        1 aaagaaaaaa taataaaagc attgcaattt ctatactaaa gagtcatgga aaaatatcta
       61 gtatatattt agtattggca tgttaattgc ttatatatta aagtataaaa atttaacaat
      121 aatttggagg tattttagat gttaaaagaa attaaatgga aagtaaataa tttgccaaag
      181 ggcgataagg aaaattgtat caaattttta aatgaagaag aaataactaa ggttagaaat
      241 tttcacaaaa gctttcctca atataaagaa actccattag caaacctaga aggtttagca
      301 aaaaaattag gtgttgcagg agtatatgta aaagatgaat cttatagatt tggattaaat
      361 gcatttaaag tattaggtgg ttcttattct atgggtagat acttagctca aagattagat
      421 acagatatat ctgagttagg gtatgacaaa ttgacttcga aagaaataaa agaaaaatta
      481 ggagaaataa ctttctttac agctacagat ggaaatcacg gtagaggagt tgcttggaca
      541 gctaataaat taggtcaaaa atctgttgta ttaatgccaa aaggttcttc tgaatttaga
      601 ttaaataaaa ttaaaggaga aggtgcagat gctagtataa cagacttaaa ttatgatgat
      661 gcagtaagat tagctaatga ttatgcagaa gctgatgacc atggagtaat ggttcaagat
      721 acagcatggg acggatatga agaaatacca gcatggataa tgcaaggata tggaactatg
      781 gctcaagaag ctatagagca attaaaagaa tatggagtag atagaccaac acacgtattt
      841 gttcaagcag gagttggttc acttgctggt gcagttcaag ggtatgttgc ttcaatatat
      901 gatgaatgcc caataacagt agtagtagag gctgatgaag ctgattgtta ctataaatca
      961 gcagaagctg gagatggaaa accaagattt gttggaggag atatgccaac tataatggca
     1021 gggcttgctt gtggagagcc aaatactata ggatttgaag tattaaaaaa tcatgcagca
     1081 gcatttgttt cagcaccaga ctgggtttct gctaaaggta tgagaacatt aggtaatcct
     1141 ttaaatggtg atgaaaaggt aatttctgga gaatctggag cagttacaac tggtctttta
     1201 gtagcagcta tggaaagaga agatttagca gatttaagaa aagatttaaa attagatgaa
     1261 aattcaagaa tacttttaat aagcacagaa ggagatactg accctgacaa atatagaagt
     1321 atagtttggg atggagaata tccaagtata taaggaatta ctaaaatgca attattagat
     1381 attggtttac atatttagaa gatatataag ttctaaaatt tagaaaatat ataagtatat
     1441 aaaatacata cattataaaa aatctagggg gataatgaaa tgttaaacga acaaagaaaa
     1501 caagaagtaa ctgaaatatg ccaaaaatta attcaaaatc ctagttcttc tggaaatgaa
     1561 gaaggtgttg taaaagcaat agaagagtct tttgaaaaat taggttttga tagttggtca
     1621 agagatagat atggaaatat agtaggatgt ataaaaggta acaagccagg taagaaaata
     1681 ttatttgatg gacatataga tacagttcca gttccagatg caagtaaatg gagtgttcct
     1741 ccttttgaag gaaaaatagt tgatggaaag atatatggta gaggaacttc tgacatgaaa
     1801 ggtcaatata ctgcaatgat gtctgctgta gcgtattttg ctaaagacac taatagagat
     1861 tttgctggag aactatatgt agcaggtgtt gttcatgaag aaatatttga aggtgtttcg
     1921 gctagagaga taagtaaggc tgttcaacct gactatgttg taataggtga gtcttcagag
     1981 ttaaacttaa agattggtca gagaggtaga ggagaaatag tagtagaaac ttttggtaaa
     2041 ccagctcact ctgctaatcc agaaaaaggt
//
LOCUS       DAYASV010000259         2051 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYASV010000259 DAYASV010000000
VERSION     DAYASV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.264"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            129..827
                     /locus_tag="ACGI68_003757"
     CDS             129..827
                     /locus_tag="ACGI68_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140157.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            1005..1895
                     /locus_tag="ACGI68_003758"
     CDS             1005..1895
                     /locus_tag="ACGI68_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140158.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
BASE COUNT          876 a          204 c          351 g          620 t
ORIGIN      
        1 gttgaaaata atgctcgaac ctataaaact attaactaat atttgtaaat gtatactttt
       61 gatgataaaa tataattaag ttaagttggt ttttataaac ataaatttat tttaaggagg
      121 tgaaataaat gaaaatatct agtcaatata gaagtcaata ttcatttaga tatgaaagta
      181 atataaataa tacaagaata aatgaaagta tggttaagaa aaatgaaact gtaggaaaag
      241 acacttattt atctaatatt atgaaacaaa agcaagaact taatgataga attagagatt
      301 taaaatatag acaagaggtt tatactaaaa aaataaatga cgcaattaag aacttatgta
      361 aatcagaaat aagagaaaca actaataatt tttctaatat agaaataggt attaaaaata
      421 gcattataga agagaaaaat aaaagtacaa tgttagatga aaattcaact tatctaaata
      481 caaatgatga aaaagaatct ttaatcacta aagagtctaa tgaaaaaatt gaagaagaaa
      541 tattaaatga tgaaaaatta gaagagttag aacaaaaaaa ggattataaa gaggattcta
      601 ataaaaaaga gaaagtatct gaggacttat ctttagtagg taaaactcgt gaagagcttg
      661 aaaatatgct taaaaatttt ataaatttaa cacaagaaga aataatgaaa cttgagtcga
      721 gaatagaaaa gttagataaa aatgctgaag aatacaaaca aaattcaaag actaatatat
      781 ttgataaaac agatgaacaa aaaaaacata taaatgtact gatttaaaca atatgtttta
      841 aaaaaataag tttattatat aatgaaaata taaaggtaca atttcaatac acttctatta
      901 aaccaataat ttatgaacca gacttaattt caatttacta ataaccatat atttatgagt
      961 taatagtttt atgttttaaa aagaattaaa taagggggag tagtatgaag aagaatatat
     1021 taactactat gattaccatg attattatat tgatgatgag tatgacagta atggcagagc
     1081 catatcaaga agatgaaaca tctagcttga aaaaacagag tgcaaatgaa cgtaatcaga
     1141 ctaatatagg cacaatggat ggcagagtgc aaacaaatgc aataagtaaa ggtgctaatt
     1201 atgaccctaa atatcctctt aaaggaatgt taacacaact tggtcttaat acaacaggta
     1261 aagagggatg ggatttactc tatggaggaa atcccaatgg agttgagctt cgagcagatg
     1321 gtaaatatta ttatataaat aagggtatgg aagggctagg attttatgcg gcagcattgt
     1381 caggtggctc atatcagaga ataggctttg gaaaacttga ccctatagaa gtaatagctg
     1441 atggaaattg gatttcttac aaacaagctc aagatacact tacagtaatc aggaattttt
     1501 taaatagctt tgactggaga aatgcatctg agatggaaag agctaataga gctgcaaaat
     1561 tggtaaccga agcaaaatat gcagactcta aatattgtaa catagtctat ggtaatctgg
     1621 ttgataaacg tggtgtatgt ggaagttttg caagtagctt tcatttatta acaaggttaa
     1681 tgggaatgga ttcactatct atattaaatc catcattaaa tcatgcttgg aactatatac
     1741 aaattgatgg gaaatggtat aggtttgatg ggtctgaaat atcagctttt ggtggatcat
     1801 tagactttga ttaccgtaaa ttaaaggatg caacaagaga gatgactact tactatgatg
     1861 ctaaggcatt gtctatatta ggatttaatc aataaaaata gggggaggct tcttatagag
     1921 ccttttttta ttgatttgaa atttaataag gaattttata tgaaaattta atcaataaaa
     1981 gcaaaaaagg ctctttataa agcctttcta agtgtttttt taagtaaaat tttttatatc
     2041 aacatcagtt t
//
LOCUS       DAYASV010000260         2044 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYASV010000260 DAYASV010000000
VERSION     DAYASV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.265"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            476..1816
                     /locus_tag="ACGI68_003759"
     CDS             476..1816
                     /locus_tag="ACGI68_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFH0140159.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCM
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKINKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          819 a          148 c          310 g          767 t
ORIGIN      
        1 gcttttgttg taagaatcat tttatctaat gattgttttt tataatagtt aaatttccat
       61 ttgtagtgtt taaatataaa tctataacag catttctata aatgttataa atcactataa
      121 tgattcaact caaagttact gcttaaagaa gcagagattt tatcaaggta tatcatggaa
      181 aaaaatattt tattgtaata tttataagat tttattatag aaataatgta aaaactttat
      241 aaataatata ttttatacct agttatagat aaatttatat agttattatt aaaaaaagca
      301 ataaaaagtt taattcaatt tagcaaattt agtgatataa ttaaaaaaga aaagaaatat
      361 ggacttattt ggaataattt tgacaatcta tttactacat gtgtagagtc gtatataatt
      421 aaattataca taaaatataa aaaaataatt tagtaatacg ttaggagaaa gtattatgat
      481 aaagtcaagc ttattttgga atattttcat tgcgatatcc tttatgatag aatggtttct
      541 ttgtaagaaa atgctagatt atacaagtga gaaaaggatt tctaacaaaa aaataaactt
      601 ttgtatggta ggtattattg tttttgctga cataatatat ttttttgatg tacctccaaa
      661 tataagagta attatatcta tgataattac tattgcattt tataaaatct catatgatgt
      721 ggggtttata aagtctgtat taatatcctt aatttattgg atgttactaa tcgccacaga
      781 tgctttgagt atatctttaa cagtttggct taattctata aatgacatga gtagtatggg
      841 gacgggagat atatacagaa ttgagtctat aatattggga aagatacttt tgattgtgtc
      901 tttgctttta tatagagcct taaagataaa acttaatata gaaataaata aaaggacatt
      961 attttatata gttattccta tagtcgctaa tatagcatct ttttttgtaa tttttgaata
     1021 catatttaat ccagggtctg gtagcttaat acataatttc caattcgtta tgatttctgt
     1081 tgtattgctt atatcaagta tcactttagt tttaattgct aaaaaattac agcaatataa
     1141 tactttagtt gtagaaaaca atatgattaa aaatactttg aagtatgaaa atgagtatta
     1201 tgaagatata gaaaagaaat atttaaaaac tagattacta agtcatgata tgaaaaatca
     1261 catattgtgt ataaatgcta tgattaaaaa aggtattgat gttacttctt atttaaataa
     1321 tttgcaggta gaaattcaga gtaatgactt gttatttaat actggtaatt ttatattaga
     1381 tgctatatta agtgaaaaga aagaggtttg tgataagaat gatatagaac taaaaatagg
     1441 aataaatttt agttgttgtg aagctgttga attagtggat atatgtcata tattttcaaa
     1501 tattttagac aatgcaattg aggcatgtaa taaaattgat agtggaaata gagttatttg
     1561 tgttaaaggt gatgttatgc aaaattttta tttaataaga gttgaaaata ctaagataaa
     1621 taagattatt actaaaaatg gagatatttt gacagacaaa aaaggttctc ttcatggact
     1681 tggaataaaa agtgtaaaaa actctgtaaa taagtatggt ggtaatgttg taattgaata
     1741 tgatgataaa aaatttgtag ttaaaattgc tattccactt agaaatgtga agaaagcagt
     1801 taatcatgtg atttaaatgt atataaattt gatgtgtaga aatttaatat ataagaatta
     1861 atgtataaaa gtgatgcata gaagtttaat atataagaat taatgtataa aagtgatgca
     1921 tagaagttta atatataaga actaatgtat aaaagtgatg cataaaagtt taatatataa
     1981 gaatttaata tgtataaaag tgatgcataa aagtttaata tataggaatt taatatgtat
     2041 aaaa
//
LOCUS       DAYASV010000261         2007 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYASV010000261 DAYASV010000000
VERSION     DAYASV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.266"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            126..407
                     /locus_tag="ACGI68_003760"
     CDS             126..407
                     /locus_tag="ACGI68_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140160.1"
                     /translation="MPNIEKDLDGTTTITYLNDDIGVPTDENGYKISMTILHGKTICH
                     VVSPETILGRKFTLEEKQEKDTIIYGAIEKIMVDRQLQIKNSNKEVASH"
     gene            557..1405
                     /locus_tag="ACGI68_003761"
     CDS             557..1405
                     /locus_tag="ACGI68_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFH0140161.1"
                     /translation="MIKSFLMLGQSNMAGRGFISEVTPIYNERIQMLRNGRWQMMTEP
                     INYDRPVSGVSLAASFADAWCCENQEDRIGLIPCAEGGSSLDEWNIDGILFKHAISEA
                     KFAIQSSELTGILWHQGENDSNNGNYKFYYKKLLSIIETLRKELNIPDIPIIIGGLGD
                     FLGKVGFGKSCTEYVFINQELQKFAFEQDNCYFVTATGLTSNPDGIHIDAISQRKFGL
                     RYFEAFHKKKHIMEALANESELIIPSNSKTYTKTEKIYINSMDLALGKISYDEFESKL
                     IKLNDN"
     gene            1457..1978
                     /locus_tag="ACGI68_003762"
     CDS             1457..1978
                     /locus_tag="ACGI68_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFH0140162.1"
                     /translation="MIPLLILGLLKQNPNSYGYELLALMEKKHYKYIVNFTKGSFYYN
                     LQQLEEKKFIEKVENKQSNKNRETHNYIITELGCQEFEKLMYKYGSKTEYINLSFYAT
                     LLFANDYKNEKLIELLETQIKQTQVRISLLNESIENDKTNSHYFNRMLENSRSHHLVN
                     IEWFEKLLNDIRD"
BASE COUNT          841 a          222 c          319 g          625 t
ORIGIN      
        1 atttatagtt aactattttt gaagtttacc cttaatcaag caacacttag ttattcaatt
       61 ataaataatg attgagaata ggacaatttt taagatacat aaaatataaa gaggagggga
      121 tttaaatgcc aaacatagaa aaagacttag atggtacaac tactataaca tacctcaacg
      181 acgatatagg tgttccaaca gacgagaatg gatataaaat atctatgaca atactacatg
      241 gaaagacaat ctgccatgta gtatctcctg aaacaatctt aggaagaaag ttcacattag
      301 aagaaaaaca agaaaaagat acaataatat atggagcaat agaaaaaata atggtagaca
      361 gacaattaca aataaaaaat tcaaacaaag aagttgctag tcattagcac ttctttaata
      421 agtaaagtag gacaagtatt ttttataata ttattgactt atagtctaat tagactataa
      481 tataaacata gatagtctaa ttagactact attaaaaata aatacaataa gaatttaaga
      541 aaggataata ataataatga ttaaatcatt tttaatgtta ggacagtcca atatggctgg
      601 aagaggattc ataagtgaag tgactccaat ttataatgaa agaatacaga tgttacgtaa
      661 tggaagatgg caaatgatga cagaacctat taattatgac agaccagttt ctggggtaag
      721 tcttgctgca tcatttgcag atgcatggtg ctgtgaaaat caagaggata gaattgggct
      781 aattccttgt gctgagggag gcagttcctt agatgaatgg aatatagatg gtatactttt
      841 taaacatgct ataagtgaag ctaaatttgc catacaaagc agtgaattga caggaatttt
      901 atggcatcag ggagaaaatg acagtaataa tggcaactat aaattttatt ataaaaaatt
      961 actttctatt attgaaactc ttagaaaaga attaaatatt ccagatatac caattattat
     1021 tggtggattg ggagacttct taggaaaagt aggttttgga aaaagttgta ctgagtatgt
     1081 atttatcaat caagagttac agaaatttgc ttttgaacaa gataattgtt actttgttac
     1141 agcaacaggt cttacatcta atcctgatgg tattcatatt gatgcaatat ctcaaagaaa
     1201 atttggatta cgttattttg aagcttttca taagaaaaag catattatgg aagcattagc
     1261 taatgaaagt gagttgataa taccgagtaa ttccaagaca tatacaaaaa cagaaaaaat
     1321 atatataaat agtatggact tggcactagg aaagatatca tatgatgagt ttgaatctaa
     1381 acttattaaa cttaatgata attagataaa atattcatta atatactaat aaatatagat
     1441 tgaaagaggt gattttttga taccattatt aattttagga ttattaaaac aaaatccaaa
     1501 ttcttatgga tatgagttat tagcattaat ggaaaagaag cattacaagt acatagtaaa
     1561 tttcactaaa ggttcttttt attataattt acagcaactt gaagaaaaaa agtttattga
     1621 aaaagttgaa aataaacaat ctaataaaaa tagagaaact cataactaca taattactga
     1681 acttggatgc caggaatttg aaaaattaat gtataagtat ggttctaaaa cagagtatat
     1741 aaatctatca ttttacgcaa cactattatt tgcaaatgat tataaaaatg aaaagttaat
     1801 agaattattg gaaacgcaaa ttaagcaaac tcaagtaaga atttctctat taaatgaatc
     1861 catagaaaat gacaaaacaa attcacatta ttttaataga atgctagaaa attctcgttc
     1921 tcaccattta gttaatattg aatggtttga gaagttgcta aatgatatta gagattgatt
     1981 ttaataataa aaaaacatac atatata
//
LOCUS       DAYASV010000262         2007 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYASV010000262 DAYASV010000000
VERSION     DAYASV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.267"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            194..388
                     /locus_tag="ACGI68_003763"
     CDS             194..388
                     /locus_tag="ACGI68_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HFH0140163.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(490..999)
                     /gene="vanZ1"
                     /locus_tag="ACGI68_003764"
     CDS             complement(490..999)
                     /gene="vanZ1"
                     /locus_tag="ACGI68_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HFH0140164.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LIPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1319..1708)
                     /locus_tag="ACGI68_003765"
     CDS             complement(1319..1708)
                     /locus_tag="ACGI68_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HFH0140165.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEEDIDD"
BASE COUNT          741 a          271 c          197 g          798 t
ORIGIN      
        1 taataaaatt ttataatcta ttcaataata tacttttttc tataaatatt ttacccataa
       61 cctttatttt tttattaaaa tgcattataa taaaaagtaa atctaaattt gatacagttt
      121 taacaatcat tattttcaga aaagtgatac tattgaatat attaagaata attagagatg
      181 gaggaactaa gatatgagcg taattgtaat gtgtagtcgt tgtggaaaag aaatagataa
      241 atatagaaat aaatatataa cttattatga gaaaaatacc aatgcagaaa ttaaagtttg
      301 tataaattgt gcgttagatt tattagaaaa aacaagagaa aatcaagaat tgaacataca
      361 agagtttcaa gatacaataa ttctttaata atttaggaaa gcacctgtat aggtgctttt
      421 tatgtgtaaa taattttata tgtaactgta tcttacgttc tattttaagc taaacatata
      481 tacttatttc tataagtttg caaaaataac taatcctaaa aataatacaa caataattgt
      541 cacaatcaat gccaatacat tgataatttt tatggtatta ttcttaaata ttttcgataa
      601 tagcataaaa acagctattc ctatgattcc tcctagtgtg tttcctatca aatccgttat
      661 atcacttgca cctagtgcaa atatatattg aagtgtttca aataccaaac tagttataaa
      721 aataggtata acttttttta taaagctcca ttcttctttt agcatacaca catatatccc
      781 aaatggtaca aatatagcca catttaatat aatctctgat atatctactc tattattaat
      841 tataagtgac ccagcaaaag gtattaagtt tatgcttcta agattcatgg tttcaagaga
      901 tgagatatca aattgcaact taaatagtat tatccatgtt aaaataatta tgtaaactat
      961 aaataatcct tttgtaatat tatgttttct agatttcatt tattacctcc ttactatatt
     1021 attgtattat tttattaata caataatata gtaaggtaat ttatctgtca actatttctt
     1081 ctaaaattat attcctttta taatttcaat atctttatca tatttacatg atttaagcta
     1141 ttgtagtatt tcatatatct ttaaatttta attggtatta ttctctatat tttttactat
     1201 caaacactaa ggaaataatc acttttcttt atataatgtt ctaccatcac ataaaactaa
     1261 cattaaatcc aaacgctact ctcaattgtc tcaaactaaa aagatactga tagctttatt
     1321 agtcatcaat atcttcttct ttcaaatttt taaagtattc ttctaatttg tccaagctct
     1381 tactatctaa aacttcatca tcatgtagtg ctgaaataaa actcttaaat gaattgttat
     1441 gcataaaact aaaaaaactc tttgtctcaa cttttaagta atctttctct tttatagcta
     1501 tagtataatg cgtacatcta tctaccttat cagtagttaa aaagccctta tctactaaat
     1561 tttttaaaaa tgtcaatgtt gtgctctttt tccaatcgta tttcaacttc atgtcttcta
     1621 tgatatctct tgaggctaat acatcatcta ctccccaaat atacttcatt agttttagtt
     1681 ctgcatgtgg taatttatta atcttcatta gtttacacct cttaccaatt tatgtataac
     1741 taaactttat aacaataagt gtatgtaatc aattagattg gaataaatat aaattccaat
     1801 ttattctata taaaaataat taataaaaaa gaagtactct actttagaga gtacttttgg
     1861 attatatatt caaaaattta tctaacatga ttagagtatg ttttatgctt tagtgtgata
     1921 attttagttc gttttattat tgcaacatgt attatatttc attataatct ataagcttaa
     1981 atacaggata tttatttcat atactta
//
LOCUS       DAYASV010000263         2002 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYASV010000263 DAYASV010000000
VERSION     DAYASV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.268"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            200..814
                     /locus_tag="ACGI68_003766"
     CDS             200..814
                     /locus_tag="ACGI68_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFH0140166.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            832..1194
                     /locus_tag="ACGI68_003767"
     CDS             832..1194
                     /locus_tag="ACGI68_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0140167.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1300..1797
                     /locus_tag="ACGI68_003768"
     CDS             1300..1797
                     /locus_tag="ACGI68_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFH0140168.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          756 a          220 c          334 g          692 t
ORIGIN      
        1 caactttata atataaaact tataataaaa gtaaatttat atgttaaatg aggttgattt
       61 tttattattt tttatttatg ctagtactaa agacttgcaa acgttttttg acaagtcttt
      121 attttgtcta aattaaacaa acttttttac aaataaatac ataaaattta aactaatcta
      181 tttattggag gagataaaat tggaagaagt aattgcaaaa attattacta tagaaaatgg
      241 attcaaagaa attgaaaaca ttgctaaaaa aattgtaaaa aataatgact caaaaaagtg
      301 ttattatcta tcaatctact tatataggtc agaatattat caagtaagag aattggctgt
      361 gtttatactt ggttttatat cagtaactat ttctgaagct ttgttatttt taaaagataa
      421 tgtgagtaaa gatgaaaact ggagagtaca agaaatacta gctaaatcat ttgattatta
      481 ttgcagtgaa gtaggatatg aaaaggctct tcctgttata aaagaatggc ttaattctga
      541 tagtccaaac ataaaaaggg ctgtgacaga aggtcttcga atttggacta gtagagacta
      601 ttttaaatct aatcctgacg ttgctatatc actactttct agtttaaaag aagatgatag
      661 tgaatacttg agaaagtctg taggtaatgc tcttagagat ataagtaaaa aatatccaga
      721 tttagtaaaa aaagaattag attcatggga tatatcaaat aaaaaagttg agtttgtata
      781 taagcttgct tcaaaatgta tcttggcaaa gtaaaatgga ggtgaactta tttgaaactt
      841 cgtgatgagt atacttgccc attagaacta acacatgata tgataaaagg caaatggaaa
      901 tctattatat tatggagatt acgcatagga ccaacttcct tatcaaaact tgaaaaggaa
      961 atcaatggta ttaatcaaaa gatgttactt gaacaactta aagaattaat tgaatttggt
     1021 cttgtagata aaaagacata tgaaggttac ccacttaaag ttgaatactc acttacacct
     1081 attcgaggga aacaaatatt ggatgctttg gctataatgc aagatatagg aattagcttt
     1141 atgttggata atggtatgga agatattctt aaggaaaaag gtgttttaaa ataaatagat
     1201 acccacaaaa aagtaagtaa tttaccatta ttttcaactg gtatataatt aaattataga
     1261 tatattctat taatttatta taataggagg taaattatta tgaaagtaac tagtacaggt
     1321 atagtaaatg gtgttataga agataaatac ggtaaaagag gaactcagtt caatgaaggt
     1381 ggtatgccta catattcact acctctaaag tttgaagatg ctccaaagaa tacagtatcc
     1441 tttgctgtat ttttagaaga taaagactct gttcctgtat gtgggtttgc atggatacat
     1501 tggttagctg taaatataac aaaagatgag ttaaatgaaa atgaaagtgt aacagctact
     1561 gattttgtac aagggactac tagttggcat ggcaaactag gaggtcttga cagattagag
     1621 gccagtgtat atggtggaat ggctcctcca gacagacctc atgtctatga aatacatgta
     1681 tttgcacttg atactatcct tgaccttgaa aaaggattct atatgaatga atttttcaaa
     1741 gctattgaag ggcatgtttt agatacattt acactaaaag gaagttattc taattaatta
     1801 ggttgactat agctaatcat taattaaatg ctacatttaa ttgatgatta gcttaattgt
     1861 tattagaata ttttctgcac tttatcttaa tatatatttt agttgactgg tattttaatt
     1921 tctattagat tattatcaat ttatataaat tgattttagc ttaaaactta cttatctatt
     1981 tcagtttcta ttagaattat ta
//
LOCUS       DAYASV010000264         1963 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYASV010000264 DAYASV010000000
VERSION     DAYASV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.269"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            117..491
                     /locus_tag="ACGI68_003769"
     CDS             117..491
                     /locus_tag="ACGI68_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0140169.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            488..1351
                     /locus_tag="ACGI68_003770"
     CDS             488..1351
                     /locus_tag="ACGI68_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140170.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1351..>1963
                     /locus_tag="ACGI68_003771"
     CDS             1351..>1963
                     /locus_tag="ACGI68_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HFH0140171.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISS"
BASE COUNT          743 a          205 c          302 g          713 t
ORIGIN      
        1 tacatttatt aaatgtataa tattggtaat tagttttttc taaaatcagt tgacatatta
       61 tatatatatt tatataatgt gtatatacta tatatacaat ttaaggaggt gtcttattga
      121 atatagttat tagtaattcg agtgggaagc caatatatga acagattact acacaaatta
      181 aaagtatgat tataagtgga gagttaccag aaggctcagc tcttccaagt atgagactat
      241 tggcaaagga acttagaata agtgtaatta ctacaaaacg agcatatagc gatttagaac
      301 gagacggatt tattgagaca gttactggta aaggcagttt tgtatctagt aaaaacatgg
      361 attttgtcaa agaagaacaa tttcgtttaa ttgaagaata cctacaaaaa gcagttgata
      421 cagcaaaaag tagtgatatt acatatgacg aattaaaaga tattttattt ttattatata
      481 aaggagaatg ataagtatgg atgcaataac tataaaaaat ttaaataaaa cttataaaga
      541 ttttagccta caagatatat ctttttcagt accaaaaggg agtgtaatgg gatttgtagg
      601 tgaaaatggt gcaggaaaaa caactacatt aaaagcaatt ttaaatttaa tatcttatga
      661 tagtggaaat atagaaatct ttggtcttga tagtaaaaaa aatgaaaaag aaattaaaga
      721 gcaaattggg gttgtatttg agggaagcaa ctttcatgaa aatttaaaca ctgaccatgt
      781 ttcaaaaatc atgagtaaaa tctataaaaa ttggaatgac acattattta aagactactt
      841 aaaaaaattg agagtaccag acaataaatt aattaaggag ttttcaaagg ggaataagat
      901 gaaacttagt attgctgtag ccttatctca taaaccaaaa ttattaatac tagatgaggc
      961 aactagttca cttgacccta tcgttcgtga agaaatacta gatatatttt tagattttat
     1021 acaagatgaa gaacactcta ttatcttatc ttcacatatt acaagtgatt tagataagat
     1081 agcagactat attacattta tacataaagg aaaaattgta tttagtgaaa ataaggatga
     1141 attaattgat actatgggag ttttaaaatg taaacctagt gattttgata acctttcaag
     1201 agaggattat tcatattatc gtaaaagcca atttggatat gaagtacttc ttaaagataa
     1261 gcatagattt atctcaagac atccaaactg tattgttgat aatacgtcta tagaagaaat
     1321 tatgttattt tatgtgagag gtgataaata atgaaaggtc taattttaaa agatttactt
     1381 aatttgaaag gaaatataaa gtttatactt ttattcatca ttatgtttgg ctttatgtct
     1441 tctttaggag atggaaatgt taataacttt ataggtgtta ttatagtttt atgtactaca
     1501 atgattgttt caacattttc atatgatgat ttgaataaat gggatagtta tgtacttaca
     1561 atgcctatta atcgcaatga tattgtatta agtaaatatt taactatgtt aatatttagc
     1621 tttataggtg ttcttgtttc acttattgtt agtgtaacta ttggttattt taaaaataca
     1681 cttatattaa atgaaacact acttattaat gcactaatct tatcaatttc agtatgtttt
     1741 ggtagcctta ttctaccact tatttataaa tttggtacag aaagagctcg tttattaatg
     1801 attttatgct ttttagttcc aacactagct ttattagttt ttaaaagtat tttagaaaat
     1861 attagttcac ctatttctat agaaattata cttaatacac tagtatattc tcttccattt
     1921 gttgctattt tactatttgt gatttcatat tttatatcat caa
//
LOCUS       DAYASV010000265         1925 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYASV010000265 DAYASV010000000
VERSION     DAYASV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.270"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            119..235
                     /gene="rrf"
                     /locus_tag="ACGI68_003772"
     rRNA            119..235
                     /gene="rrf"
                     /locus_tag="ACGI68_003772"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            242..316
                     /locus_tag="ACGI68_003773"
     tRNA            242..316
                     /locus_tag="ACGI68_003773"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:274..276,aa:Asn,seq:gtt)
     gene            321..395
                     /locus_tag="ACGI68_003774"
     tRNA            321..395
                     /locus_tag="ACGI68_003774"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:354..356,aa:Glu,seq:ttc)
     gene            401..476
                     /locus_tag="ACGI68_003775"
     tRNA            401..476
                     /locus_tag="ACGI68_003775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:434..436,aa:Val,seq:tac)
     gene            482..558
                     /locus_tag="ACGI68_003776"
     tRNA            482..558
                     /locus_tag="ACGI68_003776"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:516..518,aa:Asp,seq:gtc)
     gene            569..643
                     /locus_tag="ACGI68_003777"
     tRNA            569..643
                     /locus_tag="ACGI68_003777"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:601..603,aa:Thr,seq:tgt)
     gene            658..742
                     /locus_tag="ACGI68_003778"
     tRNA            658..742
                     /locus_tag="ACGI68_003778"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:692..694,aa:Tyr,seq:gta)
     gene            752..825
                     /locus_tag="ACGI68_003779"
     tRNA            752..825
                     /locus_tag="ACGI68_003779"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:784..786,aa:Gly,seq:tcc)
     gene            836..912
                     /locus_tag="ACGI68_003780"
     tRNA            836..912
                     /locus_tag="ACGI68_003780"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:870..872,aa:Arg,seq:tct)
     gene            922..997
                     /locus_tag="ACGI68_003781"
     tRNA            922..997
                     /locus_tag="ACGI68_003781"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ttg)
     gene            1009..1084
                     /locus_tag="ACGI68_003782"
     tRNA            1009..1084
                     /locus_tag="ACGI68_003782"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1042..1044,aa:Lys,seq:ttt)
     gene            1087..1175
                     /locus_tag="ACGI68_003783"
     tRNA            1087..1175
                     /locus_tag="ACGI68_003783"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1121..1123,aa:Ser,seq:tga)
     gene            1193..1283
                     /locus_tag="ACGI68_003784"
     tRNA            1193..1283
                     /locus_tag="ACGI68_003784"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1227..1229,aa:Ser,seq:gct)
     gene            1292..1368
                     /locus_tag="ACGI68_003785"
     tRNA            1292..1368
                     /locus_tag="ACGI68_003785"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Pro,seq:tgg)
     gene            1454..1529
                     /locus_tag="ACGI68_003786"
     tRNA            1454..1529
                     /locus_tag="ACGI68_003786"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1487..1489,aa:Trp,seq:cca)
     gene            1590..1666
                     /locus_tag="ACGI68_003787"
     tRNA            1590..1666
                     /locus_tag="ACGI68_003787"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1624..1626,aa:Pro,seq:tgg)
     gene            1673..1749
                     /locus_tag="ACGI68_003788"
     tRNA            1673..1749
                     /locus_tag="ACGI68_003788"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1707..1709,aa:Ile,seq:gat)
     gene            1753..1828
                     /locus_tag="ACGI68_003789"
     tRNA            1753..1828
                     /locus_tag="ACGI68_003789"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1786..1788,aa:Phe,seq:gaa)
     gene            1836..1912
                     /locus_tag="ACGI68_003790"
     tRNA            1836..1912
                     /locus_tag="ACGI68_003790"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1870..1872,aa:Met,seq:cat)
BASE COUNT          484 a          442 c          524 g          475 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc taaaaaaaaa taaaaaaagt
       61 attgacaaaa atagtaaaaa atgttaatat tatacttgtc ctagcaacaa gaaaaaaatg
      121 tggttataat agcggagaag atacacctgt ttccattccg aacacagaag ttaagttctc
      181 tagcgctgat ggtacttggt gggaaactgc ctgggagagt aggacatagc cacgtgaatg
      241 ttccaaggta gctcaacggt ggagcaaccg gctgttaacc ggtaggctgt gggttcgagc
      301 cccacccttg gagccattat ggctcgttgg tcaagaggtt aagacaccgc cctttcacgg
      361 cggtaacagg ggttcaattc ccctacgagt caccatttgt gggatcatag ctcagctggg
      421 agagcacctg ccttacaagc agggggtcac aggttcgagc cctgttggtc ccaccatttg
      481 cggcctggta gttcagttgg ttagaatgcc agcctgtcac gctggaggtc gagggttcga
      541 gccccttcca ggtcgccaaa ataaaaatgc tggtgtggct caacggtaga gcagctgact
      601 tgtaatcagc aggttgtagg ttcgattcct atcaccagct ccatgtaaaa ctaatacgga
      661 ggatttcccg agcggccaaa gggggcagac tgtaaatctg ttgtcatcga cttcggtggt
      721 tcgaatccac catcctccac caattaaata tgcgggtgta gctcaatggt agagttctgg
      781 ccttccaagc cagctgtgag ggttcgatcc ccttcacccg ctccaaagaa agcgtgggtg
      841 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      901 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      961 catgtatgcg taggttcgag tcctgcatcc ccagccaaat aaaaatatgg tctattagct
     1021 cagtcggtag agcacctgac ttttaatcag ggtgtcccgc gttcgagtcg cggatagatc
     1081 accaatggag aggtgtccga gtggtttaag gagctggtct tgaaaaccag tgacttcgaa
     1141 aggggccgtg ggttcgaatc ccaccctctc cgccaaaaaa atgactgaat ttggagaagt
     1201 actcaagtgg ctcaagagga tcccctgcta agggattagg ctgggtaacc ggtgcgaggg
     1261 ttcgaatccc tccttctccg ccattcaaaa acgaggtgta gcgcagtttg gtagcgcaca
     1321 tggtttggga ccatggggcc gggggttcga gtcccttcac ctcgaccaac ttaagctttt
     1381 agttcagttg gttagagtgc ccaactcatg gctagtaatt ctagggtttg agtccatgaa
     1441 ggttattctt cataggggtg tagctcagtt ggtagagcgt cggtctccaa aaccgtgcgc
     1501 cgggggttcg agtccctcca cccctgccaa ataaaaagga cttgaaaaaa gttggatttt
     1561 agagtagtat aagatagtaa ataagatttc gaggtgtagc gcagtttggt agcgcacatg
     1621 gtttgggacc atggggccgg gggttcgagt cccttcacct cgaccaagaa aagggcctat
     1681 agctcaggtg gttagagcgc acgcctgata agcgtgaggt cgctggttcg agtccagcta
     1741 ggcccaccat ttgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc
     1801 ggtggttcga ttccgcctct gggcaccagg aatacggcgg tatagcttag ttggctagag
     1861 cgttcggttc atacccgaaa ggtcataggt tcgactccta ttaccgctac catctaacta
     1921 tatca
//
LOCUS       DAYASV010000266         1781 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYASV010000266 DAYASV010000000
VERSION     DAYASV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.271"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(800..1117)
                     /locus_tag="ACGI68_003791"
     CDS             complement(800..1117)
                     /locus_tag="ACGI68_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140172.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLDITIIAIILVATITVILYYLVRKRY
                     ITI"
     gene            1280..1453
                     /locus_tag="ACGI68_003792"
     CDS             1280..1453
                     /locus_tag="ACGI68_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140173.1"
                     /translation="MNDATKVSIKNETNNIMNLCNLLSWNALLQNNKINAETIAKSRY
                     FIIILSPIDFYRL"
     gene            complement(1465..1740)
                     /locus_tag="ACGI68_003793"
     CDS             complement(1465..1740)
                     /locus_tag="ACGI68_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140174.1"
                     /translation="MTLLELYYRKAEINKIVWARIGFNLYLSKGVALTLTDAGIGISG
                     LLAGAIPGIGQYLPLIVRLITMYLESNITSGKIFKFKKYVIIKEVLV"
BASE COUNT          637 a          225 c          179 g          740 t
ORIGIN      
        1 tttaaatatt tttggaaatt atatatatta taataattat agcaaatttt tagggtataa
       61 tttttaaaat tatattctac tatcaaaaga tatgaataaa tgattgtata tattatatca
      121 atttttatat ttcatagtcc attttttcat cacattttcg cttgtcaaga ataatatctt
      181 taagagtgaa gcgtttgatg taaatctgga ctttgttttt gagacatttt tgaaatattt
      241 attaaatctt tcgattatac ttgtaatata tattatagat attacttatt ttagtaaaac
      301 taaaggatga tgctacaaca ttccagttat ttcccaaaat tttaatttct gaagtatact
      361 gtttacatta tttttaatct attttatcta aagtcgataa ggcttcttat tcattaactg
      421 acctatatac agatttaaaa ttacttatta attcttttga gtccttgtat gaaacatatt
      481 taaagtattt cttatctaat gtattaaaca tttttgtatt acagaatgag gataacttgt
      541 agctattgtc tctttgaaat aagtcatctg tgtttactcc taaaactact tatatctgta
      601 gattttataa gttctccatc ttatataatt tctttgatta attgttttct taaattattc
      661 ataaaaaaat ctccgttaca atatttgatt atatttaaat cattgctata aagaagactt
      721 ttattaactt tgaaaaacaa attattttaa ttgctggtga ttagttgcta ccttcttttg
      781 atattaattc tattagattt tatatagtta tatatctctt tctaactaag taatataata
      841 ttactgttat agtagctact aatattattg ctattattgt aatatctaat ctttttgtaa
      901 ataatcctat tgctatatat tcaaaacctg ttacatatcg ataaagtact tctagtttaa
      961 tagttctttt tctatctata tttccatatt ttgattctaa tttattaaaa tatttttcat
     1021 tacaaaaacc aattttaatt tcaattaaag atattattgt agttaatatt ataattccca
     1081 atcccataag tataagataa atgttcctaa aattcataga agatacctcc tttataatat
     1141 aatcatcaac tcatgttgta ttaattttcc taatcaaagt ataacagtaa cacaagtata
     1201 aagagtatct aaaatattac aaatattaaa tgagaaaaag atggtaagtt aaccccacca
     1261 tcttattttt tatctactaa tgaatgatgc tacaaaagtt agtataaaaa atgaaacaaa
     1321 caatataatg aacttgtgta atttacttag ttggaatgct ttattacaaa ataataaaat
     1381 taatgctgaa acaattgcaa aaagtagata ttttataatc attttatcac ctattgattt
     1441 ctacagattg taagtagtat ttggttacac aagcacctcc tttattataa cgtatttttt
     1501 aaatttaaag atttttccag atgttatatt tgactcaagg tacattgtaa ttaatctaac
     1561 tataagtggc aagtactgtc ctataccagg aatagcacca gccaaaagtc ctgaaatccc
     1621 tataccagca tcagttaaag ttaatgcaac tcctttagaa agatataaat taaaacctat
     1681 tctagcccaa actattttat ttatttcagc ttttctataa tataattcaa gtagtgtcaa
     1741 tatcgttctt cttaaggttt ttaattagct tatatattgt t
//
LOCUS       DAYASV010000267         1697 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYASV010000267 DAYASV010000000
VERSION     DAYASV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.272"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            54..1652
                     /locus_tag="ACGI68_003794"
     CDS             54..1652
                     /locus_tag="ACGI68_003794"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HFH0140175.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDIDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
BASE COUNT          653 a          210 c          271 g          563 t
ORIGIN      
        1 tataatattt ttatcaatta ttattatatt caagttttga aaggagtatt aaaatgagtt
       61 tattagtttt cggacataaa aatccagata cagattcaat atgttctgca atttcattag
      121 cacatttaaa gaacaaacta ggaataaaag caacagctta tgctttagga gatatcagaa
      181 aagaagctaa atatgccttg gattacttta aggtagatgc acctgaaatt ttagataatg
      241 ttagaataca agttagagat ttaaattatg ataaagtagt acctttaaca ccaacttcat
      301 ctatattaga ggcttataac cttatggatg aaaaaaatgt taaaacacta cctgtaactt
      361 atgatgatgg aacttttgct ggtatcatta ctatgaaaga aatagctaaa aatctacttc
      421 atcaacactt tacaactata cacacatcac tatcaaacat ttgtaaaaac cttaatggta
      481 ctattcttgt ggactgtggt gaagatataa aaggtagagt agtcacttta tcatttggaa
      541 tggagactct aatagagact ttaaaagaag gagacatagc tattgttgga gatagatatg
      601 atagtattga atatgctatt gatacaaaag ttaaactttt aatacttact aatcatacag
      661 agatttcaaa agatttgcta gctcttgcta agataaatgg agtgtctgtt gtttctgttg
      721 aaagtgacaa ttataaaact tctaatgtaa taaatcaatg tagtttctta gactctatag
      781 aagctacaga aaatttaatt acattcaaag aagatgatta tatggatgaa ataaaagaga
      841 ttatgctaga aacaaacttc agatcttatc caatattaga tgatgataat aagtttttag
      901 gtttagtttc aaaaggtcat ctattaaacc catctaaaaa gaatgttgtt ttagttgacc
      961 ataacgaata tgctcaaagt gctgatggaa ttgaacaagc aaacatagtt gaaataattg
     1021 accatcataa acttggtggt atttctactg atgttcctat gtcatttaga gttatgcctg
     1081 ttggatgcaa tagtactata atttatcaaa tgtacaaaga aaataatgtt gagatacctt
     1141 atgaaatagc cgggcttctt ttatctgcta tattatcaga tacattatta tttaagtctc
     1201 caacaacaac tgatatagat aagaaagctt gtgaagaatt aagtaaaata gcaaaagtag
     1261 acatggaaaa atacgctatg gatatgttta aatgcggtac ttctttagat gaatatacta
     1321 tagaagaaat agtaaatatg gactttaaag aatttaatat gagtggacat agagttggta
     1381 ttggacaagt atttacctta gacatagact ctatatttgc taaaaaagat gagtttttat
     1441 cttatataaa ttcgactgat tatgacaaac taattcttgc ggttacagat ataatcaaag
     1501 aaggttctta tttaatctat aaagcagaag acagcttaat tgctaacgca tttggtgttg
     1561 aaggtgttca aggtacattt gcaccaggtg ttgtatcaag aaaaaaacaa ttagtcccta
     1621 atttaactac tgcaatcaaa aattttaaat aatataaaat ttttaaataa cgaaaactgt
     1681 ctcaaatcta tatttga
//
LOCUS       DAYASV010000268         1638 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYASV010000268 DAYASV010000000
VERSION     DAYASV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.273"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            60..1298
                     /locus_tag="ACGI68_003795"
     CDS             60..1298
                     /locus_tag="ACGI68_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HFH0140176.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYITYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          696 a          144 c          278 g          520 t
ORIGIN      
        1 ttatataaaa atacttatct agttactagc aattattagt agaaatgggg gaaatagaga
       61 tgaataaggg attttacaaa ttaaattcag tggtattgaa ctttgatatt aagaatgatg
      121 ttaacaagat aagatttaat cattatataa tgagaaaaga aaacatacaa agacttcaag
      181 gaactctgcc aaacggacaa ttttatatga cagtgagaag agctgctaca gatttacaac
      241 tttctatttc tactataagt aggcttgtaa aagagttttt ggatttaggg ataataagat
      301 tagtgtctaa gggtgtaaaa ggtaatcgtt ctgtatatag ttacgtaagt atagagttta
      361 atggaagttt atttgatgat agtaaatata tggaacttga ctgtgtatgt aatgatgaat
      421 ttattgataa tagtggcata gagtttgatt gtgatagcaa agataattct tttaatggag
      481 atataaatgt agaaactgtg cattgcatta atgagtattt ctctgatgat gatgtagagc
      541 ataattataa caatttccat aacaaagata ctataaagga taaagccatt tctaatatta
      601 gcactatcaa aaaatatatt gatgaatctt ttgatacaaa taaatcagaa aattctaact
      661 tacaacagga gtcaataaat ttcaacttta atgatacatg caatgaaata gataaaagaa
      721 cgaataatag tatagaaaat aatcaaaata aaaggcctat gagattgagt gacatcagtc
      781 ttttaagtga taaaggtatg acaaataaaa agaattactt taatcataat catggtatac
      841 aaaaagggac taaatctgta acaaagaaaa aagaatcctt aaataaattt aataaaaaaa
      901 atatacagca aagcattata aatatgctaa ataaaaaatc aggtaagaat tttaaaatcg
      961 attctccaat aagcataaaa cttatcaatg aaaggctaaa ggaaggctat gtcttagaag
     1021 atttctacaa agttattgag gtaaaagtgg ctaagtggaa aaataccata atggaaatgt
     1081 atataagacc agaaactttg tttagctata agtttgaatc ctatgtaaat gaaaacatat
     1141 ctatgaaaag tcaaagtaat gttcacccat gtaaagaagg caatagtaat gatagcagta
     1201 attatataca ttctgattat ataacatatg atggcagtgt gcgtgagaga aaaaaatatg
     1261 agaatgtaag atactggaat cctatatgtg aaatttaatt agtttaaaaa tgtatttatg
     1321 ataaataaga tattaataga gaatagtgag ctatagcaaa tgatatatca atagttggta
     1381 gtaagttata ttaagtaaga tattactatc aggtagtgaa ttatattgaa cgatatatta
     1441 ataattggca gtaagttata tcaaataaga tattactata gagtaatgaa ttatattgaa
     1501 cgatatatta ataattggca gtaaattata ttaaataaga tattactata gagtaatgaa
     1561 ttatattgga cgatatatca atagttggta gtaagttata ttaagcaaga tattactatc
     1621 aggtagtgaa ttatattg
//
LOCUS       DAYASV010000269         1609 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYASV010000269 DAYASV010000000
VERSION     DAYASV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.274"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            393..1598
                     /locus_tag="ACGI68_003796"
     CDS             393..1598
                     /locus_tag="ACGI68_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFH0140177.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKG
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
BASE COUNT          606 a          204 c          214 g          585 t
ORIGIN      
        1 ttcctttttt caaggacaag taatactata tcatctttat ctattctagt caatataatt
       61 ttttactttt tttatttttt ttatttatca tgtattcttt atatttaatt tttaaaactt
      121 aatgctaagt aaacctgttt atttcataaa actaatatat attctatgct ctattttcct
      181 ttttattcta tatttaaatt ttttatttac aagaatattt ttaatataac atatttgata
      241 aaattaattt acatttcttt cacttgtaat ttttgcaatc ttgtattaga ttaatattaa
      301 aaataattta tcaataacct tattcacaaa gacagttttt atatatcaaa atataatata
      361 aatactatgc tacattttag gaggatttta ttatgagcaa aaaagtatta ataatgtctg
      421 cttccactgg tggtgggcat aatagggctg ctctagctat taaagaagaa cttacttcca
      481 aaacacttga tggagagcca attgaatgtg aaataataga tagtttgaaa ttagttaata
      541 atacaatgga taaaataatt tcaagaggat acgaaaaatc tgctatatat actcccaaag
      601 cctatggaag tgtatataga ttatctgaaa caaatctttt atcaaaaaat gaatttaaag
      661 ataatctact aataacattc atggctaaaa aatttaaaaa acttattaga agcgaaaaac
      721 cagacttgat tataggaacg catccttttc caatgattgc tctaagtaca ttgaaaaaaa
      781 actttaattt acataataat gaatcaaatg cttacacaga gcatttttat aaacattata
      841 caaataccat taatgttcct ccgcttattt ctgtattaac cgattatact actcattcta
      901 catggataca aaatgaaatt gattactata ttgttggtca tgaatatgta aaagaattat
      961 tggtgtttga tggagttgaa ccgagtaaaa ttagaacatt tggaatccct gttgaaaaat
     1021 cttttctctc tcacagagat aaaggtattg ttctttctga gttgaattta tctcctgata
     1081 aattaactgt tttacttatg gggggtagct ttggggctgg aaatataaaa gagacacttg
     1141 atgaattact ggatactgat agagactttc aaatacttgt aattactggt aagaatgaaa
     1201 gcctaaaaga aaaaatcgaa aaaaaactta tgtctcgtta tcatgataaa aatgtatgtg
     1261 ttcttggata cactgataag atgaatgata ttttagcttc tatagatgta ttagtaagca
     1321 aacctggtgg acttactaca actgaggctc ttttaaaaga tgtacctatg atagttcctt
     1381 attatatacc aggtcaggaa gaagagaatc ttgatttctt atctaattgt ggtgcagcac
     1441 ttagaactac aaaaaaatat aacttgtcaa tattactaaa agttttaata gatgacccta
     1501 gcagattaga aatgctaaaa agaaatatta aatcaataag aaagtcaaat tcagcccaaa
     1561 atatagctaa tttaatttta aatataattt ctggttgata aaataaata
//
LOCUS       DAYASV010000270         1569 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYASV010000270 DAYASV010000000
VERSION     DAYASV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.275"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            252..986
                     /locus_tag="ACGI68_003797"
     CDS             252..986
                     /locus_tag="ACGI68_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0140178.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMHSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
BASE COUNT          686 a          146 c          230 g          507 t
ORIGIN      
        1 cgaattatca aaaataaaat agttattaga aaaaaataaa agtaattgca aagcttgcaa
       61 ttacttactt ctatattaat tactatacca taaaataaca aaaaaatcaa gtcgagtaat
      121 ccttcaactt aagtgatata ctaggtatta ataattttta attaataaaa tataactaga
      181 ggaaaatcta cattttctca atttctttaa ttaaattgac ctctaattaa gtaattataa
      241 ggagaaaaat tatgaattta gcaaagggat tagatgtttt aaaaatttca tcaaatgtat
      301 tcggtgaaga taaagttatg tatattcctg taatctacac agaagatgat gcaacactta
      361 ttgacacagg tcttccgggt caaggtgatt taataattga tgctcttaat aaaagtaaca
      421 cgagctttga taagttaaaa aatataataa ttactcatca tgatatagac catataggaa
      481 acataaatta tctaagagaa aaatctaaga ataatatcaa agtatatgca tacaaaagtg
      541 aaatcagcta tataacagga gaagaaactc cttttaaact ttatatgttg gaacaaatgg
      601 tagataaaat tgatgataag atgcttagta tgctaaatgt tatgggactt ggatttaaat
      661 catcatatac taaagtagat gtgtcattag ataaccatga aaaattgaat ctaggtgagg
      721 aaatagaagt tatacataca ggaggtcaca ctagaggtca catatgtctt tatttaaaag
      781 agtctaaagt acttattgct ggagatttgt tacaggtaga aaatggagaa cttaagcctg
      841 ttgatgtaat gcacagcaac aaacaagagt taaaagatgc tattaaaaat ataagtaatt
      901 atgatataga aacgatagtt tttagtcatg gtggattata tcaaagaaac ataattggaa
      961 cattgaaaaa cctaattatt gagtagtact atatgattta caataatttc ttaaattact
     1021 taaaattata tctaaagatg tagaatcaaa attaaatata agtaataaga attttgcttt
     1081 aaaattatac ttaagtgtta agggtgatac gtagtattac aagtatttta gaatatagtt
     1141 ttttatagta attttaaggt tctacaatac ttcagttgaa aaatatttaa cagcatagta
     1201 taatgggaaa gaaaatactt taaataaact ttagtataca aagtgaagaa aaatatttaa
     1261 attatatata atattgtaga gtgaggtaaa atatttaagt tatatatcat aatacagagt
     1321 gaagaaaaat atttagataa tgtaccataa tacaaggtga gtaaaaatat ttaaataatg
     1381 tatcataatg caaagcgaag aaaaatattt aaataatgta ctataatgca gagtgagtaa
     1441 aaatatttaa ataatgtgtt ataatacaaa gcgaataaga ttatttaaag catgtattat
     1501 aatataaagt gcgtaaaaat atttaaagca tgtatcataa tacaaagtaa agaagattat
     1561 ttaaaccat
//
LOCUS       DAYASV010000271         1565 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYASV010000271 DAYASV010000000
VERSION     DAYASV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.276"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            4..402
                     /gene="tnpA"
                     /locus_tag="ACGI68_003798"
     CDS             4..402
                     /gene="tnpA"
                     /locus_tag="ACGI68_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFH0140179.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            399..1562
                     /locus_tag="ACGI68_003799"
     CDS             399..1562
                     /locus_tag="ACGI68_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0140180.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNKGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
BASE COUNT          672 a          166 c          277 g          450 t
ORIGIN      
        1 tttatgtata aatctaacaa aaatatagtt tatgattgca aatatcatat tgtattttgc
       61 cctaagtatc gtaaacctgt tttagttggc gaagttgaaa agatgttaaa agaaatacta
      121 ccatataaag cagatgaact tggagcagaa ataatagaaa tggaaactga taaagaacat
      181 gtgcacttat taatatcttg tgaccctcaa tatggaattc ataaagtagt aaaaggttta
      241 aaaggcttta gttcgagagt attaagagag aactttccac atttaaagtc tagtatgcct
      301 agcatgtgga ctaatagcta ttttgtaggt actgttggtt ctgtgagttt agaagtagtt
      361 aaacaatata tagaaaatca acaagttagg acaaagaaat gataaaaact catagagtga
      421 aattaaacct aactagaaca caatttgaat tagtaaggga aaagcaaatg gaaagtgcta
      481 actgttggaa ctacatagtt aacttatcta aagaatacta ttttgaacat aaacaatgga
      541 ttaaaaagaa tgatatacaa aagttaatca aaggtaagta caatcttcat agtcaaacta
      601 ttcaagctat atcagataaa tttgatgcaa atagaaagac tatatcagaa ttaagaaaaa
      661 aaggtaatac taaagcaaaa tatccataca agactaagaa attttatata atacctttta
      721 aagctagtgc tattaataga aatgctaaag gcaacttaaa attatcaatg tcaaaaggca
      781 ggtatttaga attagatttt aatgttgaga atataaaaac tgctgaaata gtatggcgaa
      841 atggatatta tttatactac acttttgaca atgaattaaa tggagttgta gccaaaggca
      901 ctaataccgt aggtgtagac ttaggggaaa tacattcgat agctagtgta acaaataaag
      961 gtgtagggct tattctatct aatagagaag gcagaagtat taaacaattt agaaataaga
     1021 tgtatgctta tatatctaaa agattaagta aatgtaaaaa aggtagtaga caatcaaaga
     1081 agttatggag attaaaaaat aagataagaa gtaaaacaga taatcagtta atgaatttat
     1141 atcatcaaac aactagaaag tttatagact tttgtgtaga gcagaaggta tgtgaaatag
     1201 ttcttgggga tataaaaggg gttgaaaaag atactaagaa aaagaaaaga ttaaacagag
     1261 ttaatagaca gaaaatatct caaatggagt atggtcgaat aaaggactat ataaaatata
     1321 aagcaaaaga gcaaggtatt gaagttaagt tagttaaaga aaactacact tctcaaactt
     1381 gcccaaagtg ttctaaaaaa cacaaaccaa ctggtagaac ttatagttgt acttgtggtt
     1441 atgaaactca tagagatata gttggtgctt ggaatatttt aaataaaaag cataaatatg
     1501 gtttagtaga ttttagaata aatcataagc aaccaataaa tttaaaagta tctaccgttt
     1561 gagcc
//
LOCUS       DAYASV010000272         1537 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYASV010000272 DAYASV010000000
VERSION     DAYASV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.277"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            109..843
                     /locus_tag="ACGI68_003800"
     CDS             109..843
                     /locus_tag="ACGI68_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0140181.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGTNLHWPVDGYFEEGVRKSNFLGEEVIKYHKTLTTYLNTLMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(986..1477)
                     /locus_tag="ACGI68_003801"
     CDS             complement(986..1477)
                     /locus_tag="ACGI68_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFH0140182.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
BASE COUNT          555 a          185 c          232 g          565 t
ORIGIN      
        1 aatttgttta tagaaacttt tattatttta tataaaagtg ataaaaactt atattttgta
       61 tttaatagag tgttgctaag tgaatatatt taaaatggag gaaactttat gaaacaaaat
      121 aaatatgatg atgatagatt ttttaataaa tacagtaaaa tggaacgttc tacaaatgga
      181 ttagcaggtg ctggagagtg gcatgtttta aaaaaaatgt tacctgattt caaaggaaaa
      241 agagttttag atttaggatg tggatttgga tggcattgtc aatatgcagt tgagaatgga
      301 gcaatttctg ctgttggagt tgatatttca gaaaaaatgt taaaagaagc tagaaacaaa
      361 acgcaacttg ataatataaa ttacatatgt atgcctattg aagatattaa ttttccaaaa
      421 gatagttttg atgtagttat tagttccttg gcatttcatt atattcaatc atttgaagat
      481 atatgtaaga atataagtaa ttgtctttca aataaaggta attttgtatt ttctgttgaa
      541 catcctatat ttactgcata tggaagtcaa gagtggtact gtgataaaga tggcactaat
      601 ctacactggc cagtagatgg atattttgaa gaaggagtac gtaagtcaaa ttttctagga
      661 gaagaggtta taaaatatca taaaacatta acaacttatt taaatacgtt aatgaaaaca
      721 ggatttgaaa tattagaaat tattgaacct caaccagaag aaaaactgtt agatacaatt
      781 cctgatatga aagatgagct acgtagacct atgatgttac ttgtatctgc aaaaaaaaga
      841 taagtaaata taaataatca ttattgtaga aaaaaagttg tagaaaataa ttacactaat
      901 ggagatgatt ataccagtgg tattaattac accagtagta aggaggcaaa agcattcgtt
      961 gcttttgcca ttttttaagt tgcaattata cttctacgta gtatatttcg tttccatttt
     1021 cttctataag ctttttcata tcttcaaatt ttaaaaatac agtagcagta ttatcatttg
     1081 gatgtatacc tattttatcc atttttaata aatctttatc aaatacaact tttacaacag
     1141 cctctttatc atttaatata ccaaaaggag tcacagctcc tttagtaagt ttcaagtatt
     1201 tttgaagtct ttcttccgat gcaaaactaa gaggtttact atcaatttgt tgacgaatat
     1261 cttttaaatc tgcttttttg tctttaccta atacaactaa aaaatgttct tttcctttag
     1321 catcacgaag aaataggttt tttacaacaa attccatatg taatgttttt aattcttcca
     1381 tttcttcaat tgtataaact ggtggatgat ttacagcctc ataaggtatg cttaactcat
     1441 ctaatttttt atatacttcc tgttgttgat tgttcatgat ttcctcccat tagaaattta
     1501 ttttatatat aaataatatt atcacagaaa actttaa
//
LOCUS       DAYASV010000273         1531 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYASV010000273 DAYASV010000000
VERSION     DAYASV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.278"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            169..1305
                     /locus_tag="ACGI68_003802"
     CDS             169..1305
                     /locus_tag="ACGI68_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFH0140183.1"
                     /translation="MIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLIN
                     EIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYMI
                     YNNHRALEYGLDNLDELYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEIIH
                     TGLEPLKIYDFISKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYLY
                     LIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEMR
                     NMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLKA
                     GIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1331..1519
                     /locus_tag="ACGI68_003803"
     CDS             1331..1519
                     /locus_tag="ACGI68_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140184.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
BASE COUNT          662 a          130 c          219 g          520 t
ORIGIN      
        1 cctgtaacat atattttaaa tgcttattct taaatttttg cattatttat taaaatttat
       61 tatgctttaa cgctaaattg agtttgataa ttataaaaca aataatcaaa aatagtgtaa
      121 aaattaatac tttttgttaa tattaataga taaagataat ctaaggtggt gattaaactg
      181 acaatagaag aatacaaaaa caaatacttt actggaaata aaatcgtact ttctataaat
      241 agttttgata ataaagaact atactatcat gaaactgaaa atatattaaa tttaataaat
      301 gaaatagata aattaacatc aagtaagata tttaaaacaa tagaagataa aaatgatttg
      361 gcactagagt cttattattc tagtgtaata gaaggtgctt tttcaactag aaagattgca
      421 aaatctataa ttagaggaaa gatgaaacca agtaataaaa gtgaatatat gatttacaat
      481 aatcatagag ccttagaata tggattagat aacttagatg aattgtatag tcacaaattt
      541 atatatgatt tgcatcatat attgggcgaa aactgcttgg attcagaaga atatgagtat
      601 agaactgaaa aagtatatgt atgtgattca aaaggtgaga ttatacatac agggcttgaa
      661 cctttgaaga tatatgactt tattagtaaa ttaatagatt ttatggaaaa tagcaaagta
      721 agtaatttaa ttaaaagtgc tataattcat ttttactttg tatatgttca tccttttagt
      781 gatggtaatg gaagaacatc aagagcctta tcatatctat atttgattga taaaggatat
      841 gatactttca aggatttttc aatatcatat atgatatcta aaaacagaac taagtattat
      901 aaggctatac ttgatgtaga aaacaaagga aataatttaa ctgtatttat agaatttatg
      961 ctcaagtcta taatacaatc tattaatgag atgcgtaata tgcatgatag aaaatcttta
     1021 gaaagtatat taaaagaaga attgtttgaa aatgacataa ctctatcagc aacagaagaa
     1081 catatattaa aatatatttg caataaagat aactattcta tgacattaga aaattatata
     1141 aaaaagaata aaagtagata cttaaaggct ggaataaagg aaattgaatt agttgaccag
     1201 ttgatggaag tgtttaataa tcttgaagag atagagattt tgtctaaaga aaaaaatata
     1261 tataaagtta atgataagta cttaaaaatg cttgatatag actaatttag ataatctgga
     1321 gagggaatat atgaataata agaaaaaggc atttttgatt tctggggcta taaacgtaat
     1381 ttcattaatt attgtttttt cattgtttca ttataatatt atagaaaata gtacacaaaa
     1441 tatgataata actgtaatct taatttttat ttttgaaatg ataaaaataa aaatcataga
     1501 gaaatactat aaactataaa caagaaatat c
//
LOCUS       DAYASV010000274         1478 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYASV010000274 DAYASV010000000
VERSION     DAYASV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.279"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(61..1353)
                     /gene="brnQ"
                     /locus_tag="ACGI68_003804"
     CDS             complement(61..1353)
                     /gene="brnQ"
                     /locus_tag="ACGI68_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFH0140185.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLFVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT          528 a          277 c          185 g          488 t
ORIGIN      
        1 tccttttact tttagttaac tcatttattt acaattgatt atattttaat taaacaaaac
       61 ttagtttgtt ccaagtgtat tagattgact ctttgatggt ataaaattac caattattcc
      121 agctattata actggcacca cccagttaaa tcctaatgaa ttaagaggta aagaatttac
      181 tactggaaca gctactccat aactagatat aactgttagt atacttacaa acaatgacat
      241 atatactgct cctttgtata cattatcatt cttaattttt tcattgaaga aagctaaaat
      301 tattaaaaca attgatggtg gataaactat acttagtata ggagatgcta tctttattat
      361 agttccaaca ccaaaagatg ctactactgc actgaataca gatacagcta atactatttt
      421 ttcataactc aatttacctt ttgtaagtct agagaaatat tgacctgtgg cagatgtaag
      481 acctatagat gtagttaaac atgctaatga tactactact gctaaaatcg ctttcccaac
      541 atttccaagc aatccttcag ttatatttac tattacttgt gattgtactg catctgttcc
      601 atacattgtt gatacagttg ccccaaggaa acataatcct ccataaacta gagctaatcc
      661 tccacaagca agaacacccg ccttaccaat cattttcaac ttgtcactaa tagtagaata
      721 tcctttatca tttaaagata ctattagcac tgatgcaagt gctattgctg caaaagcatc
      781 cattgtctga tatccttgac ctataccttc tgcaaataca ttaggtatca taggtttatc
      841 tactatatct ccaagaggtg atataatacc ttttataatt ataaatgcta aagctattag
      901 aagagctgga gttaaaaact gtccaactat atctacgacc ttagaaggtc ttattgttag
      961 tacatatgaa atcgcaaaaa aaagtataca gaataataac actggaattg aagttccaaa
     1021 caaaggcatt atacccattt cataagttgt tgcacctgtt cttggtatag ctaagaatgg
     1081 tcctatacat aaaatatctg cacaacctat aagaattcct aacttcatac cacatctttt
     1141 aaacatttca actacgtttc catcgaattt agctgtagcc attactgcta gaagtgccaa
     1201 tccagcatca gcaaacgtaa atcctaagaa ggcaattaac cattctggac ctgtaattat
     1261 tcccaagtaa ggtggaaata ttaagttacc tgctccaaag aacattgcaa ataacgcaaa
     1321 tccaaatact ataacatcct tggtcttacc cattataaaa tccccctttt taaccttatt
     1381 aaattaataa aatgtctcga ttgtttacgc ttgcataagt aagttgaatt aaacaataag
     1441 aacttattat taaattaata aaattaaata aattaaat
//
LOCUS       DAYASV010000275         1469 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYASV010000275 DAYASV010000000
VERSION     DAYASV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.280"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            152..397
                     /gene="cdd1"
                     /locus_tag="ACGI68_003805"
     CDS             152..397
                     /gene="cdd1"
                     /locus_tag="ACGI68_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Cdd1"
                     /protein_id="HFH0140186.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(615..884)
                     /locus_tag="ACGI68_003806"
     CDS             complement(615..884)
                     /locus_tag="ACGI68_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0140187.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT          592 a          215 c          167 g          495 t
ORIGIN      
        1 tcagtgattt agaggtggta aaatatatat atatgaaaga gcataaataa attgatataa
       61 taaaaaatta aatattaaat attaactata ataaaaaaat atctggtaat aggtataata
      121 taaaaagaat ataccagaaa gaaggtgaag tatgggagaa ctacaaaaaa ttccaggggt
      181 agggaaagca acagaaaaat ctttaatcat gttaggatat acaactatta aatcacttaa
      241 agatgcaaac ccagcacaaa tgtatgaaaa ggaatgttta atgcgtggac agcacataga
      301 cagatgtcag ttatatgtat acagatgtgc agtatatttt gcatcaactg aaaatccaga
      361 accagaaaaa ttaaagtggt ggtattggaa agattaaaaa atcaaaaaag ttagacataa
      421 aaaagagttg aatatccgtt ttaaataata aaaactaata ctcaactctt ttttagtcgt
      481 aaattttatg taaatctatt tgtatggcta aatataccca tcaataaatg tataaatata
      541 ctaatcaaca aatgactaaa catactacct aacaaaggat taaataaatt ccttaacaaa
      601 gcattctaaa acaattaatt agaaactgtt tcttcaagcc attttctaca ttgctcaata
      661 tttaccatag ggtcaaaacc ttggtctttt cccttattta aaagcatatc acaagggaaa
      721 gtatcacatt caccacaatg attaagttcc ttactttcac agcaagtttt aaccttacat
      781 tctccacccc aaaatggttt ttccatagtt gggcaaccag tgcaatttac ttgttctttt
      841 tttgtacaac tgtcacaaca gacaccacat cttgattcaa acatattcaa acatcctttc
      901 cattatttaa aaatattata aaatgatttt agatgaatac aaaagtgcaa atagttgatt
      961 ttaggtcatc taaatgttaa acataaaatt attagtactg gtaaatttat aaaaactatt
     1021 ccaaaaatct ttttgaatat ttcaaaacac aataaattac ttcaaatccc cttgaatata
     1081 ttttacagca ttatagattg ctttaaatct ctttgaatat tctacaagac tatagattag
     1141 tttaaatccc tttgaatata ttttacaaca ttatagattg ttttaaatct ctttgaatat
     1201 tctacaagac tatagattag tttaaatccc tttgaatatt tcaaaacacc atagattact
     1261 ttaaatccct ttgaatgttc tataacacta taaattactt ttaactttta tataaccata
     1321 aagtttttat ctggcacttt aaatggagtt ttatatcaaa tttgccttcc atcgaataac
     1381 caaacttaat ttaattacac attccaagag tcaaatattc attcaatata tttgataatt
     1441 ctatgttata gctataatta aatatagat
//
LOCUS       DAYASV010000276         1389 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYASV010000276 DAYASV010000000
VERSION     DAYASV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.281"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            211..432
                     /locus_tag="ACGI68_003807"
     CDS             211..432
                     /locus_tag="ACGI68_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HFH0140188.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            432..695
                     /locus_tag="ACGI68_003808"
     CDS             432..695
                     /locus_tag="ACGI68_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HFH0140189.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            757..1329
                     /locus_tag="ACGI68_003809"
     CDS             757..1329
                     /locus_tag="ACGI68_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFH0140190.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
BASE COUNT          542 a          163 c          244 g          440 t
ORIGIN      
        1 ttgtataaac tctatattat ttatttaaaa tagatattta tgaaagatta attagaaaaa
       61 ttttagaggt aaaataagaa aaaaagatac ttatttaatc atttaatctt attaaagacc
      121 tattaaaact accagttata atatagaacc agtatacaat ttttaaaaat aatgctattt
      181 taaaattcta aaaattaagg aggataatta atgggatatt taaaagagga taaacaagac
      241 tattgttgtg aatttgctag accatacatc aatcgtcaaa tttatgatga cacatacaat
      301 ccaaaaacag cattaaaaag aggtacttta tttcctgaat tagatttaat agagtcaaat
      361 aactataatg attggctata tgcaaatcct aaaaatagag ttagagctaa aaaagggggt
      421 aacatgtact aatgtcatta aatagaagag aacttttaga gagaatttca gaatatcaat
      481 ttgcctgtat agaattaaat ttatatttag ataacaatcc aagggataag aaagctcttg
      541 attcatacaa cagatactgc gataaattta cacaagctgt gtgtgattat gaatctaaat
      601 atggagcatt aactaatttt ggatatgaat caagtgaata tccttggtca tggatatctg
      661 aaccttggcc atgggacaaa tcattttata aataaaatta ttaaacttta aaattagatt
      721 tattattaaa tatatattaa ggaggagtaa gataatatgt ggatttatca aaaaacactg
      781 gaacatccag ttaacataag acaagcagac cctagaatgg caaaatatat catgactcag
      841 ttgggaggac ctaatggtga gttggcagct gcaactagat atcttcaaca aagatatact
      901 atgccaactg gaaaatctcg tgcactttta actgatatag gtacagagga aatggctcat
      961 gttgagataa tttcttcagt gttatatcaa ttaataggca attgtactcc agaagagctt
     1021 aaggctgctg gacttggtag taattatgct aattttggac atggtcttca gccagtagat
     1081 tctaatggag taaactttac tacaagttat attaatgtct ttggcgattc ggtaactgat
     1141 ttacatgagg atatggctgc tgaacaaaaa gcattggcta cgtactatca attaataaat
     1201 ttaactgatg accctgattt gaaagatata ttgagatttt tgggtgagag ggaagtagtt
     1261 cactatcaaa gatttggtga agcattaatg gatgtttatg agtttacaga gtgcaagcat
     1321 cagttttaat gaagaattga aagtgaatgg agaataagca tcagttttaa taaagaatta
     1381 aaagtgaac
//
LOCUS       DAYASV010000277         1302 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYASV010000277 DAYASV010000000
VERSION     DAYASV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.282"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            138..371
                     /locus_tag="ACGI68_003810"
     CDS             138..371
                     /locus_tag="ACGI68_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFH0140191.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSIFISTKW"
     gene            567..995
                     /locus_tag="ACGI68_003811"
     CDS             567..995
                     /locus_tag="ACGI68_003811"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0140192.1"
                     /translation="MIKKLNSKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKNFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
BASE COUNT          597 a          107 c          184 g          414 t
ORIGIN      
        1 caaaattata aaaaaattct acttatataa aatataaaga ggttatatgt acatatgtgt
       61 agaaataaat aataaaaata tcaattaatg ttattagggg ggggttactc attagtaaat
      121 tagctaatac acttagaatg atgatattaa ttcaaagcag aggcaaaatg aaatgcaaag
      181 aattatcaga agagttggaa gtaagtgaaa ggcaaataaa aagttacaag atgtatttag
      241 agcaagctgg tatatttata aattcaacac ctgggattta tggtggttat gaaatagata
      301 aatgtaatag catatcatta attaaattat tagatagtga agtttcaatt tttatatcaa
      361 ctaagtggta gataagtaga atattagcaa tatcaacgag tatttagaaa cttattttat
      421 attaataaag cagtatataa aattgtagaa tcaacaatag catatactaa aacatccacc
      481 ttatattaac taaatcatat ctaaataaaa tacacttacc tacaaacatt ataaaatcaa
      541 tacaaaaatg aggtgaaaca aaatttatga taaagaaatt aaacagtaaa gacataaata
      601 aaatcatgga aatatgggaa aaaagtacaa tcaaagcaca tgactttata agtaaagaat
      661 actggcaaaa taactataat actgttaaaa acgaatatat acctatatca gatacatttg
      721 tatatgatga tggagatgaa ataaaaggat ttataagcat aatagataaa aactttatag
      781 gagctttatt tataaagccc aaataccaaa atctaggtat cggaggtaaa cttttagatt
      841 atgcaactaa aaaatataaa agtctaagct tagcagtata taaagataat aaaaaagcag
      901 ttgtgtttta taataaaaaa ggttttaata tagtaaaaga acaagtaaat gaagattcag
      961 gatttaaaga gtacataatg gaatatagta aataatatga ttacattcta ttaaaaggat
     1021 aattaattta ttttctagtt gattttttaa gagtaatttt taaaatagac tatatgaaga
     1081 taaagatatt tattatagat gtaagttaat cagaattgat atagctacaa aagaggaaaa
     1141 taaaattgta tggtgtattt cagatttaac aaatttacta tttaaagata ataagtttga
     1201 tataatatta aagatacttt cacctataaa ctatagagta ttgaaagaat tttaaataat
     1261 tatggaatgc taataaaggt ttgttaaaat ttaataatta tt
//
LOCUS       DAYASV010000278         1243 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYASV010000278 DAYASV010000000
VERSION     DAYASV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.283"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            173..1054
                     /gene="pepI"
                     /locus_tag="ACGI68_003812"
     CDS             173..1054
                     /gene="pepI"
                     /locus_tag="ACGI68_003812"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase"
                     /protein_id="HFH0140193.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
BASE COUNT          462 a          136 c          237 g          408 t
ORIGIN      
        1 taatataatt ttataattgg tttagtatag agatttttaa cttttatgtt atactattta
       61 taaaagtagg tataaagatt aaaatacata atcaaatatg tataagaggt tttaaatagt
      121 attgttaatg aacttgatta ttgtttataa ttacaaattg gaggagataa acatgaaaat
      181 tactgaagga tatatgcctt ttaaaggttt taaaacgtat taccgtatag taggagaaaa
      241 tacggaaggg aagaagccat tagtattact tcatggaggt ccaggttcta cacacaacta
      301 ttttgaggta ttggataaga tagctgaaag tggtagacaa gtaataatgt atgaccaaat
      361 tggttgtgga aattcatttg tagaagggca tcctgagcta tttaatgcag atacttggat
      421 agaggagctt attgaactaa gaaaacatct aggacttgat gaaattcatc tgttaggtca
      481 atcttgggga ggtatgcagg ctatttggta tgctattgaa tataaaccaa aaggtattaa
      541 gtcttacatc ctttcatcta ctctttcttc tgcaaaactt tgggagaaag aacaaaaaag
      601 aagaatatca tatatgagtg aggttgacca aaaagcatta cttgatgcag taaatactgg
      661 agattactct agcaaagaat ataatgatgc attagaaaga tttatggaaa tgtattgtgc
      721 aggtgaagtg acagaagatt ctccagagtg cctgagaaga cctaaaaaat caggttctga
      781 agcatatatt gtaggatggg gacaaaatga attttctcct acaggaactc tttctggata
      841 tgaatttaca gatagattac atgaaataaa agagccttgt ctagtaacta gtggagcaat
      901 agatttatgt tcaccatata ttgcaaagac tatgtatgat agaataccta atagtaagtg
      961 ggaattattt gagtattcaa gacatatgcc attcgttgaa gaaaatgaga aatatataaa
     1021 agtcttaact gagtggttga atgcgaacga ttaaaaacta tataaattta tataaaaaaa
     1081 tgagtgtctc aaaatgaact ttttagttca ttaggcactc tttttgcatg aaatcaaata
     1141 tattttcatt atttgaacga taaaatgagg cttatatcta ttttgggaaa gtaatttttt
     1201 attactagcg ttagattcta atctaattgt atacttattg aaa
//
LOCUS       DAYASV010000279         1237 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYASV010000279 DAYASV010000000
VERSION     DAYASV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.284"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            459..1139
                     /locus_tag="ACGI68_003813"
     CDS             459..1139
                     /locus_tag="ACGI68_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0140194.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKIRNITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          537 a           77 c          187 g          436 t
ORIGIN      
        1 taataatcga aattttatac tttggttaaa ataattttat atatagtgcc taatgtataa
       61 cctaatgctc gtagacaata atgtggatat tatcaaattt atttttataa tagaaatttt
      121 aatttatatc tatattaaat tgttaaagat ataattatga gaataaaaaa tatagctttg
      181 aattatataa ttgtatatgc ttatctttat acagtaaata aaaaatcaaa tgaaatacca
      241 aataaagtgt atatcttaaa ataacttact agagatacaa ggatgctatg tgaattttga
      301 agtgtgcagt ttagataata aatatatata aataataaag aaaatggatt tatagtattt
      361 aaaatttctt ataagggtaa aaaatgctat tagaaaatgt actaaaaata tttttccaat
      421 tctttataga aaaataatga tatttaggag agattgtaat gcaaattgat gtattgataa
      481 ttgatggaga taagaatagt tctcaagagc tgaaaaattt tttagaagga aaggggataa
      541 atgtctattt agcatataat tgtaaagatg ctattataaa aatatttagc aagaaatttg
      601 acttaatatt tttagagata attttagttg atggtgatgg ttgggatttt tgtaaaaaaa
      661 ttagaaatat aactacttgc ccaattattc atataactta tataaatgaa aatcaaagta
      721 ttttaaatgc tttaaattct ggaggagatg attatttaat aaagccttta aatttagaaa
      781 tattatatgc gaaagttaaa gctattttaa gaagaagaga tttattttta aataataata
      841 aaaataatat aaaactagaa gattataata gagctagtgg agtaataaag ttagaaaata
      901 aaatgataaa gttaactcca acagaaaata gattattaaa ttactttata gagaatgctg
      961 aaaagactct aactattaag gagatatatg aacatgtatg gatgaatgag tatttagatg
     1021 ataattatag tgtagtggtt gctgtgagtg gattaagaaa gaaaatagag aaagattata
     1081 aaaatcctca aaaaattata acgataagag aagtagggta ttactttaac aagatataaa
     1141 gctttataaa cagaatgaat gataaggcat agataatatg ttagtatatt ataagagttt
     1201 ttgttctaac gatatatttg taaaattaga tttaatt
//
LOCUS       DAYASV010000280         1233 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYASV010000280 DAYASV010000000
VERSION     DAYASV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.285"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            132..1208
                     /locus_tag="ACGI68_003814"
     CDS             132..1208
                     /locus_tag="ACGI68_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HFH0140195.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          483 a          173 c          216 g          361 t
ORIGIN      
        1 atataaaata aattatatat aaaaaataaa ttttatgttg acatagttaa ttcagcttga
       61 tattatagtt attaaaaata aggacagtaa aaacgctatc catattacaa actaaaaagg
      121 ggggaattat aatgagtaag aaattattta ttcctggtcc tattgatgtt aaagaagaag
      181 tcttgcaaaa gatggctaca ccaatgatag gacatagagg aaaagatgca tctatgctcc
      241 aaaagagtat aagtgaaaag atgcaaaaac tattctatac aaacaacacg atacttttat
      301 ccacgtcatc cggaactggt ttaatggaag gctccattcg ttcctgtaca tccaaaaagg
      361 cagcagtatt ttcctgtgga tcctttggtg atagatggta caaaatggca gtggctaata
      421 atgtcccagc ggatatattt aaagttgaat taggagaggc aacaacgcca gaaatggttg
      481 ataaggtgct ttctaccgga gaatacgatt taataactgt aactcacaat gaaacttcaa
      541 ctggaataag aaatccaata gaagaaattg gagaagttgt taaaaagtat gaggatgtaa
      601 tctactgtgt agatactgta agttcagccg gtggaataaa agtcgaggtt gataagattg
      661 gaatagatat ttgtataaca tcagtacaaa aagcactggg attaccacca ggaatgtcta
      721 tttgcacatt ttctcaaaaa gcaatcgata gagctaagca agtacctttt agaggagtat
      781 atcttgattt gttagcaatg tatgaatact taatcaagaa aaactaccaa tacccttcaa
      841 caccatctct ttctcacatg ttcgctttag atttccaatt agataatata ttagatgaag
      901 gcctagataa cagattcaac agacatgaag acatggcaaa cttagttaga aactgggcta
      961 aaaaacactt ccaaattttt accaatgaaa atcacttatc caatacactt actgttatag
     1021 aaaatactca aggtattagt gtatcaaatt taaattcaaa attacaagaa cgaggattcc
     1081 aaatagcaaa tggctatgga gatttaaaag aaaaaacttt tagaatttca catatgggtg
     1141 actatactgt cgaagatgta caagaattat tagataatat tgatgatata ttaggtttta
     1201 ataaataata ttgtaaaaat atgtatgttt taa
//
LOCUS       DAYASV010000281         1230 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYASV010000281 DAYASV010000000
VERSION     DAYASV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.286"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            106..1146
                     /locus_tag="ACGI68_003815"
     CDS             106..1146
                     /locus_tag="ACGI68_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFH0140196.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLSLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT          473 a          143 c          197 g          417 t
ORIGIN      
        1 ttttttttta aatatgttta gattctattc ttttaatttt agttttcaga acatttgtat
       61 ttatgttata atttgtataa atatatttat ttaggaggaa acaatatgaa tttaaattta
      121 actaatacta taaatttaat gaaaaaatat ttggacattc caagcccagc tggatatact
      181 gaaaatgcag ttttagaaat caaaaaagat tttgaaaatt taagtcttga cactattctt
      241 acaaaaaaag gtgcacttat agctactctt cctggtgaag atgattcaaa tcaaataaca
      301 atatctgcac atatggatac acttggagct atagtaaaag aaatatcatc tgatggtact
      361 cttaaatacc ataaagttgg tggtggatgt tgggcggcta tagagggaga aaactgtact
      421 gttataacta gaaaaggtaa aaaaatcaga ggaagtgttg tatttaagta tgcttctact
      481 catatatatg gtcaaacaaa agctgccact gaaagaaatg aagaaacaat gattattaga
      541 cttgatgaaa aagtatttac aaagcaagat gtattagatt taggaataag tgttggggat
      601 tttgtatgcc tagaccatag atttgaggct acagaatctg gttttataaa atctagatat
      661 atagataata aatcagctgt agcaatggtt cttgaaatat gtagatattt taaagaaaac
      721 aatttaacac ctaagcatac tactaacttt tatataagta attatgaaga aattggacat
      781 ggtgtttcta aatgtttacc tgaaaaaaca aaagaatttg tagcaataga tatagcacct
      841 actggaccaa gtcaaacatc tagtgaacat ggtgtaacta tagttgcaaa agataattta
      901 tcattatatg attttaattt aagaaacaag cttgtcgatg ttgcagaaga caataattta
      961 gactacgctg tcgatgtatt tactttctat ggttcagatg cttcagaagc tattcgttgg
     1021 ggagaagatg ttcaaatagc atgtgttggt cctggaacaa acaatagtca ccattatgaa
     1081 agaacacata tagaagctat tgaaaataca ctaaaattac ttataaatta tatgcttaaa
     1141 gaataattaa ttatatattt taccaaagtc aattcttaac aaataattaa ctttaatttt
     1201 tatttttata caaaaattgg tttttctata
//
LOCUS       DAYASV010000282         1178 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYASV010000282 DAYASV010000000
VERSION     DAYASV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.287"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(304..795)
                     /locus_tag="ACGI68_003816"
     CDS             complement(304..795)
                     /locus_tag="ACGI68_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFH0140197.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASDDGLAVIF
                     FIM"
BASE COUNT          457 a          166 c          107 g          448 t
ORIGIN      
        1 atttttatat ataatgaaaa atgtgataag tatccaaaag aattactgga tataatagga
       61 tagggtattt agccttttaa gataaataaa aatatctaca actatatatt gcaaaaattt
      121 aaaactaatt tatatatgct aatataaata tttataaaga cataagacaa atttacctac
      181 agatttaaat ttaacttata tattttaaaa ggctaatata atatgtattt tcttctatac
      241 aatcaaaagg cttcatatct aagatatgtg atgacactaa tattcacaat tttttagtaa
      301 ttatcacatt ataaaaaata ttacagctag tccatcatca gatgcttctt gtataaactt
      361 tttaagctca attagatttg cccatacata ttcaaaaaaa tcatcttcac tctcatcatt
      421 cataacagga taaatattat tttctaacat ctcattaata ttaaatcttt ttctaaatgc
      481 ttgttcttcc aaactgttca ttgcttttga tagttttttt acatcatttg ctgtaatcaa
      541 catgacagga aattctccat caatttccat aagtatgttt ccacttagta ctaatttgct
      601 aaatatatta tcatcatctc caccaaaagg acaacctgtc aaagtaaagt gaatggcatg
      661 ccatgcttta tcaatattga tagtactatt ttcgttggca tcttcaaaaa caaaatcttg
      721 taagttttct tctgcttgct gtaatttaaa aacattctct tcacttattt tagtataaca
      781 tcctatcatc cccataatta cattcctcct aagtagcgtt tttttaattg taacttataa
      841 ttaaacttag ttacaataaa atatattgag aaaagtatta tttcatctta aatttaaata
      901 cctatattat caactaaaat tctgtgttta tagcgtaagt attattattt tttcctaaaa
      961 attaaatgaa tcagaagttc tataggcaat attgactata aataagaact attactttta
     1021 aaaaaaaatt taagaaatta tactgtagaa tcatgctcta cttattagct ttaaataaaa
     1081 aataaatcat gttctcaaat taaataataa ataacaataa atttaaattt taaactataa
     1141 gagtttctga agtgttaata agcataccaa taattata
//
LOCUS       DAYASV010000283         1163 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYASV010000283 DAYASV010000000
VERSION     DAYASV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.288"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            36..845
                     /locus_tag="ACGI68_003817"
     CDS             36..845
                     /locus_tag="ACGI68_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140198.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            887..1153
                     /locus_tag="ACGI68_003818"
     CDS             887..1153
                     /locus_tag="ACGI68_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HFH0140199.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
BASE COUNT          452 a          145 c          171 g          395 t
ORIGIN      
        1 tatacttatt ttttatatat caggaggata tacttatgaa taaaataatc aataacattt
       61 taaatacctg caaacagtat acatatattt tgccacataa attgttaaga cagtatatag
      121 cacactatac aatatctatt ccagatacaa gtattaaaga aaatcttaca ttaatacctg
      181 atgctagtgg atgtatgata tttaaatttg acaaaaaagg tatagaaagc gcattctggg
      241 gagccacaac aaaaactact attgtaaaaa atgatataga aaatgtactt tttagagtat
      301 tcgttgagtt tcgcccaggt ggagtatatt atttgacagg tctttctcaa agagaatcta
      361 cagatttaaa aatttcactt gaagatttta acacattatt tagcttagag gttaattcca
      421 tttttgaaag aacaagtact ataaaggaat tagtagaaca attagatatg ttattcttat
      481 catacttatt gaaatcaaat atagtagata tgactatacc aattcttgaa aatgctagaa
      541 aacaaaactc tattatgtct gttaaaaaca tttcacaaat tagttgttat agtgaacgac
      601 atttaaatag aatttttaat aattctcttg gtatgagtgt taaatcatac cttagattac
      661 ttcgtataaa ccttgtatta caagaaatac aaaataataa aataccattt gcaactttag
      721 cacaagatat tggttattac gaccaatctc attttataaa tgattttaaa tctatttgtg
      781 gtgttaatcc aacaacctat ataaaaaatt tgtccgattt ttacaatgaa aagtataaat
      841 tttgatatac aatcatataa aatttatatt taaggagtga cttaaaatga tagaatcaag
      901 atgtggaatt ttatgttatg aatgtggata caaggaaaaa gtcaattgta aaggttgtac
      961 agagattgag aaaccatttt ggggtgaaca atgtcctgta aaatcttgtt gtgaagataa
     1021 aaaccttaca cattgtggtc tatgtgatac atttccatgt gaaatgctta atcaatttgc
     1081 atatgacaaa gaacaaggtg atgatggcaa aagaatttct cagtgcaaaa aatgggctgt
     1141 actagcctta taatatactc tct
//
LOCUS       DAYASV010000284         1125 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.289, whole genome shotgun sequence.
ACCESSION   DAYASV010000284 DAYASV010000000
VERSION     DAYASV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.289"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            239..943
                     /gene="cwlD"
                     /locus_tag="ACGI68_003819"
     CDS             239..943
                     /gene="cwlD"
                     /locus_tag="ACGI68_003819"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HFH0140200.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKECKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT          500 a          113 c          167 g          345 t
ORIGIN      
        1 taaattctga atatacgtta tcaaaattta taatataaac taagtataat ttattgtata
       61 gtattgaatg ttagataaac atagtgaatt aaatagggac agaaatataa tatcattaca
      121 taataatata ttaataccaa taaataaaga tatatcaata ttaaaaaaat ttaaatgttt
      181 gtactaatat atggaatatt atagtgtttt tataagtata catatacagg gtgatgttgt
      241 gagaaagtac ataaaacata taatttttag ttttgcaatg atatgtctag tagtagtatc
      301 catatttgaa attaaaaata tttctgaaga tgttatcaag tatatgccag taacaaacaa
      361 aactataatt ttagatgctg gtcatggagg cattgatcca ggtgcattaa ataaggataa
      421 gagtacatct gaaaaggata ttaacctagc aataacactt aagcttagag agcttataga
      481 atcaagtggt ggccttgtaa tattaactcg agaagatgac agtagccttt ataaagaaga
      541 aaataataaa actacaagac aaaaatataa tgagaatttg aaaaatagaa aagaaataat
      601 atctaattct aatgccaata tgtttgtctc tatacatctt aatgcatttg aacaatcaaa
      661 atactatgga gctcaaactt tttatcctaa agacaaacaa gacagtaaag aattatcgaa
      721 gtgtattcaa gaagaactta aaagagtagt agataagaca aacaacagag aagtcaaacc
      781 aagggatgat atatatcttt tgaaagaaaa taatattcca tcagtactga tagaatgtgg
      841 ttttttatca aatgaaaaag agtgtaaact cttaactgat gaaacatatc aagaaaaaat
      901 agcatgggca atctacatag gaatacaaaa atatttaagt tgaaataact ataaaaatag
      961 gtctaaatat gtaaatagct tgtattaaag cagttaaaat gcattaatat aggctatttt
     1021 tattttgaca aaaaaatatt taaaataaaa gttaaaaagt tgttgactta gaataatata
     1081 gatgatatta tatatgagtg cccaaaagga gcaccaaaat aagac
//
LOCUS       DAYASV010000285         1038 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYASV010000285 DAYASV010000000
VERSION     DAYASV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.290"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(2..73)
                     /locus_tag="ACGI68_003820"
     tRNA            complement(2..73)
                     /locus_tag="ACGI68_003820"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Asn,seq:gtt)
     gene            complement(80..153)
                     /locus_tag="ACGI68_003821"
     tRNA            complement(80..153)
                     /locus_tag="ACGI68_003821"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Cys,seq:gca)
     gene            complement(161..236)
                     /locus_tag="ACGI68_003822"
     tRNA            complement(161..236)
                     /locus_tag="ACGI68_003822"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Lys,seq:ttt)
     gene            complement(245..321)
                     /locus_tag="ACGI68_003823"
     tRNA            complement(245..321)
                     /locus_tag="ACGI68_003823"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(285..287),aa:His,seq:gtg)
     gene            complement(329..405)
                     /locus_tag="ACGI68_003824"
     tRNA            complement(329..405)
                     /locus_tag="ACGI68_003824"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Pro,seq:tgg)
     gene            complement(429..505)
                     /locus_tag="ACGI68_003825"
     tRNA            complement(429..505)
                     /locus_tag="ACGI68_003825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(469..471),aa:Ile,seq:cat)
     gene            complement(520..593)
                     /locus_tag="ACGI68_003826"
     tRNA            complement(520..593)
                     /locus_tag="ACGI68_003826"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(557..559),aa:Met,seq:cat)
     gene            complement(600..675)
                     /locus_tag="ACGI68_003827"
     tRNA            complement(600..675)
                     /locus_tag="ACGI68_003827"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Phe,seq:gaa)
     gene            complement(679..767)
                     /locus_tag="ACGI68_003828"
     tRNA            complement(679..767)
                     /locus_tag="ACGI68_003828"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Ser,seq:tga)
     gene            complement(856..931)
                     /locus_tag="ACGI68_003829"
     tRNA            complement(856..931)
                     /locus_tag="ACGI68_003829"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(896..898),aa:Gln,seq:ttg)
     gene            complement(939..1015)
                     /locus_tag="ACGI68_003830"
     tRNA            complement(939..1015)
                     /locus_tag="ACGI68_003830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(979..981),aa:Arg,seq:tct)
BASE COUNT          279 a          278 c          233 g          248 t
ORIGIN      
        1 gctccaaggg tggggctcga acccacagcc taccggttaa cagccggttg ctccaccgtt
       61 gagctacctt ggaatatttt ggaggcgaca cccagattcg aactggggat caaggagttg
      121 cagtccactg ccttaccact tggctatgtc gccacctttt tggtgatcta tccgcgactc
      181 gaacgcggga caccctgatt aaaagtcagg tgctctaccg actgagctaa tagaccatat
      241 gaattggggt ggataatgag agtcgaactc acgacctcca gagccacaat ctggcgctct
      301 aaccaactga gctataccca ccttatattg gtcgaggtga agggactcga acccccggcc
      361 ccatggtccc aaaccatgtg cgctaccaaa ctgcgctaca cctcgaattt atattaatta
      421 atttaatgtg gtgggccatc agggactcga accccagacc taccggttat gagccgggcg
      481 ctctaaccaa ctgagctaat ggcccacatt aaattttagt agcggtaata ggagtcgaac
      541 ctatgacctt tcgggtatga accgaacgct ctagccaact aagctatacc gccatatttt
      601 ggtgcccaga ggcggaatcg aaccaccgac acggggattt tcagtcccct gctctaccga
      661 ctgagctatc tgggcatttg gcggagaggg tgggattcga acccacggcc cctttcgaag
      721 tcactggttt tcaagaccag ctccttaaac cactcggaca cctctccaca ttcggatact
      781 taaacttatt ctaaaattga acctaactat atttaaaata aaaataattc aaatatagct
      841 tagtaataca tcatttggct ggggatgcag gactcgaacc tacgcataca tgagtcaaag
      901 tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac agggattcga
      961 accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg cacccacaat
     1021 ttatatatgt atatcaaa
//
LOCUS       DAYASV010000286         1032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYASV010000286 DAYASV010000000
VERSION     DAYASV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.291"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(91..801)
                     /locus_tag="ACGI68_003831"
     CDS             complement(91..801)
                     /locus_tag="ACGI68_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFH0140201.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT          354 a          141 c          113 g          424 t
ORIGIN      
        1 aaaattaaat ttgaaaaaac aaaactctaa aaactaagct ttaaaaagtt aagtttcaaa
       61 aataaaattc tagagattga acttcaaaaa ttaagattct aacatatacc ttgtaaattt
      121 tcctacaacc tcttgatatt ttttacggct tataggaatt ttttcattct gaactttggc
      181 ataagtttga gttatactat ctacatggtt tagattgaca atataccttt gatgcgtacg
      241 tataaaatta gagggaagaa cagtttccat atcatttaat tttccgtagt attctaatgt
      301 tttatcaaag gtaactacag ttaattttct tagctcactt ttaaagtaga ggatatcttt
      361 tgtattaatt ttacaaagat tagttccaat ctgaactgta ataaagtttt ctttccattg
      421 ttctaatttt tttagtgctt cttttaatac tttacatatt ttttcagttt tataaggttt
      481 taagatgtag ttcaaagctc ctacatcata tccatgaaat acatagtcag aaaaacctgt
      541 gacaaagatg attatagtat cttttgaatg ttttcgtata gtttttgcta tgtcaacgcc
      601 attacttttt ccaagttcaa tatctaaaaa aataatatct atgtaataat tttcgtgttc
      661 tagattgtat actagacttt ctccatttga atattctttt atttggtagg ggtagcccca
      721 caaatccatt tctcttgtaa tgattttatt cagcatgttt ctttgttgtt tttcatcatc
      781 acaaatacaa atcgaagtca attttttatc tcctttcaag ttttatattt atatagttaa
      841 gtatataata tattttgcta cctagatata taaaatgaaa taacaattat atccagatag
      901 caaaataaaa ttttttatat tagtatactt acacttaaaa ctattctatt aaacttattc
      961 tgaagtgcta atgattttct tattaaaaaa tctaacaatc tttcttatta taaaatacaa
     1021 atgcttttat ta
//
LOCUS       DAYASV010000287         1030 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYASV010000287 DAYASV010000000
VERSION     DAYASV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.292"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            367..834
                     /locus_tag="ACGI68_003832"
     CDS             367..834
                     /locus_tag="ACGI68_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0140202.1"
                     /translation="MKFLNVLVVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          398 a           68 c          168 g          396 t
ORIGIN      
        1 tatttgaata atctttttaa tcatataaaa tgcatatact tgtaatattt aaaggtgata
       61 ttaatattat tagtgtatat taatattccc ttgaaattaa aaaaacatag atatttcagt
      121 aatctaaaat agtctacaaa aataacagtt ttagcaaaat taataagaat acatatagat
      181 ttaaacctaa aagtatgaac atgtctgttt tttacaagat taaaatttat attatatgat
      241 aaaattaact taactatttc aataagactt ataatttttt tctttatact agaccttatt
      301 gaaaccttta tagtttaatt ctaattatat attataaata aagtatgtgt atattaagga
      361 ggtaatatga agtttttaaa tgttttagta gtagttgaag atattgagaa atcaaaaaag
      421 ttttattatg atgttttggg gttaaaggta atctgtgatt ttggagaaaa tgttgtatta
      481 gaaggtaata tatcattaca agaaaaaaag ctttggttag aatttattaa taagagtgac
      541 agtgaagtta agttcaatgg aaatgatgct gagttatatt ttgaggaaga taattttgat
      601 acttttgttg aaagattgag tacaatgaaa gatatagatt atgttcatct agctatagag
      661 cataggtggg gtcaaagagc aattagattt tatgatttag atgggcatat tattgaggtt
      721 ggagaaacta tgtcatctgt gtgtagacgt tttttagata gtggtctttc aatagatgag
      781 gtagctaaaa gaatggatgt aactgtcgaa tatattgaat cagttttaga atagatggat
      841 tatattaaag taaaattgag gtgaaggctg taagtaaact tgtgtgaaaa caaatttgct
      901 tacagttttt atttttctaa aataaagaat ataatacttg tttagcttta tttgagtttt
      961 taaaaatatt atgatatttc ttatatattt atactaaagt ttaaatttag aaaaaatata
     1021 taaaagatag
//
LOCUS       DAYASV010000288          987 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYASV010000288 DAYASV010000000
VERSION     DAYASV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.293"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(802..>987)
                     /locus_tag="ACGI68_003833"
     CDS             complement(802..>987)
                     /locus_tag="ACGI68_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140203.1"
                     /translation="KDGTIVPPDTETKVTLVFTVTHTSSGKTADTGEIEVTVPARTTT
                     VSSLLGRIAVNIFNFNK"
BASE COUNT          384 a          116 c          102 g          385 t
ORIGIN      
        1 atatcaaaaa tttttaatta gacttctctc ttattcaaga tttttctata taaaatctaa
       61 gaataaatag aaaactaaca tctaaccttt tattttttat tttcgatcta tttacttgaa
      121 ttttaacata aatctatctt ttagtatata ttaatcatgc tgaatttgtg atgacatgta
      181 tacaaaatat cgtttagtat tttctaataa aaaatattac tgaaattaaa aatctacaat
      241 agcatttaac aagtaaaaac atgtttacac aaatatataa tttaaggtct ttaaaataga
      301 gtatgtatta ttctaaaaat tagttatagt tatcacataa aaatagaaaa gaattttcaa
      361 aagatttaaa gaacatatat acttcaattg cagaagatgt aatgttaagt gaactagcat
      421 tcttcgagga aaaatagact caaaaaatta gaaaatagaa tcaggtttta gatgatttaa
      481 gtatttattt tcaaaaaaga aatttgattc taagttaata aagaaatatt taaaaaaatc
      541 aaaaaatttt cttaaataag taataggttt tgtaaaaatg gaaaaatatc aaaataagac
      601 tatgattttt aaaactatag ccttatcaat aaatttttat ttaaagaaca cacataaata
      661 tttttaaacc taatttatgt gaagttttat gtatttttta tataagtagt attctaatct
      721 tactcatcta atataccaaa aagactgtaa gtaagttcac ttttatgcaa acttacccac
      781 aatctctttt acacattaat tctatttatt aaaattaaaa atatttactg ctattctacc
      841 caataaagat gatactgttg ttgttctggc tggtactgtt acttctattt ctcctgtatc
      901 tgctgtttta ccacttgatg tgtgtgtcac tgtaaatact aatgttactt ttgtttctgt
      961 atctggtggc actattgttc catcttt
//
LOCUS       DAYASV010000289          969 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYASV010000289 DAYASV010000000
VERSION     DAYASV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.294"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            273..839
                     /locus_tag="ACGI68_003834"
     CDS             273..839
                     /locus_tag="ACGI68_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFH0140204.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT          404 a           71 c          162 g          332 t
ORIGIN      
        1 aggtgaaata aaaaattaat tataagtaag gcatttagaa taaaatttta aaaaaattat
       61 aattaaaaaa ataatgaaaa tatataatat tagtttagat tataaaaaat aaatatacaa
      121 atacttataa atacaagtta tatacaagat ttatagtaga gtaataatct tataaaataa
      181 tttaataaat ataaattaaa ttatatgtgt atatattgta ctttaatagt gatataataa
      241 taaaaaaatt tttaaagagg gtgatattac atatgagaaa acttgaagag atattagaat
      301 ataataaatc atttgtagag aacaaagaat atgaacaata tgtgacatca aaacatccaa
      361 ataaaaaaat agtagtctta tcttgtatgg atacaagatt aactgagctt ttgcctaagg
      421 ctatgaattt gaaaaatgga gatgtgaaac taataaaaaa tgcaggagct actataatgc
      481 atccttttgg aagtattatg agaagtgttt tagttgcaat ttacgaattt gatgtagatg
      541 aggttatggt tgtagggcat catggatgtg gtatgtgtaa tgttgatacg gataagttac
      601 ttggtaaaat tttagataga ggaatatcta atgatgtaat acttacactt cgtagtgctg
      661 gaattgatac aaaacaatgg ttacatggat tcgattctgc agaagaatct gtaaaagata
      721 gtattgattt aataaaaggg catcctttaa ttccagatgg tattattgta catggtctaa
      781 ttatatcacc tgaaactggg aagttagacg ttgttgtaaa tggatatgaa gataagtaga
      841 gtatcataat gttatataat caagatactg ttgaatatga tgttgtttca aaatttattt
      901 tgggacaact tttttatttg aaaatatatg aaaaggttta ttttaagctt tacaataaaa
      961 aatataagt
//
LOCUS       DAYASV010000290          955 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.295, whole genome shotgun sequence.
ACCESSION   DAYASV010000290 DAYASV010000000
VERSION     DAYASV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.295"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            187..759
                     /locus_tag="ACGI68_003835"
     CDS             187..759
                     /locus_tag="ACGI68_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0140205.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKGESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSV"
BASE COUNT          381 a          117 c          140 g          317 t
ORIGIN      
        1 acttagttta taaataatca atataattta tccaatatta cacattactg aaaaaactaa
       61 acataaagct gaaaattatt attaagtttt tgtagaaata ttataagcat agatacattt
      121 ttgtaacaac tacaataaat ttttaatact atatactatt cgtatgtctt atgggaggta
      181 tttattatgt ttaaaaaaat agtcgcaata gcaagctcta tcattgtaag tacaatatgt
      241 ctaactggat gtgattttaa ctctaatgac aaaaaaggtg aatcttcttc gtctaaaaat
      301 ctggataaag taactatagc agaagttact cactctgttt tttatgctcc acagtatgct
      361 gctattacga aaggattctt tgaagatgaa ggtataaaga ttgaccttat aaatacccaa
      421 ggtgcagata aaactatggc agctttgatt tcaggtgaag caaacgtagg tttaatgggt
      481 cctgaagcat ctatatatgt atataatcaa aacaaaaacg attatgcagt gaactttgct
      541 caattgacaa aaagagatgg tagttttcta gttgctagga aaaaaatgcc taatttctct
      601 tacaatgatt taaaaggaac cgaaatatta ggtggtagaa aaggtggagt tccactaatg
      661 acttttgaat atgttctaaa agaaaaaggt cttactattg gagaaaatct aaaagcaggc
      721 aatgttaatg taagaactga tgtacaaaaa tcagtatgat tataacacaa attataaatt
      781 caaactacat attttatagt tatcaattta atatttaact ttataagagc tatctcaaaa
      841 tagagataaa gctctttttc attgttaaaa tgatgaaaac atatttaatc taatactaaa
      901 atatatttaa attaatacaa agaagagtgc ctaataagct aaaaagttca tttta
//
LOCUS       DAYASV010000291          938 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYASV010000291 DAYASV010000000
VERSION     DAYASV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.296"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            59..829
                     /locus_tag="ACGI68_003836"
     CDS             59..829
                     /locus_tag="ACGI68_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140206.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLSL
                     NKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTFYCCGNCDSEISLSQREHFKI"
BASE COUNT          398 a           87 c          132 g          321 t
ORIGIN      
        1 tatataaaaa atagtaacaa atagtttata cacaagatat ttaaaaggaa gtgatgatat
       61 gaaagttaat gaaaataata tagatgaaga gttagatgaa cttagtagga aagtttactt
      121 agagtcacca gatttttgga taagtatttc tgacaaaata atagataaag tatttgatga
      181 aatgttatta ttttttgcag ttaaatataa ctatttcaat atagtatgct ttgctattga
      241 aaacaattat ctggatttaa acatgccttc aaaaaacaaa aaatacacta atataatgga
      301 gcatttgtta gatacatcaa aacagaatga agataaaaaa atttataatt atcttctaag
      361 cttaaataaa ggtaataacc tagatgaaag accaatttta gatgaattaa actgtaatat
      421 tagtagtgat acttttgaaa aaaaagatat atatcttcca aagtacttat gtccaagttg
      481 taattctaat atatttgata caggttataa agtatcaaat gacataacat ataaattttc
      541 ttctaaagaa gctgaaccta ttgaaatgtg tagtgaagtt tcttctatca gatgttgtaa
      601 ttgtgatgaa ttattagaag atattacact tgataaacta tataacctat gcaaaattca
      661 aaactgtaat aattgtggtt ctaatttaac taaggtagga attattgcaa agaaaaaaat
      721 gaactttgat gagaatactg aaaaatttaa agatgtctca acattttact gttgtggaaa
      781 ttgtgactct gaaataagct tatctcaaag agaacatttt aagatttaaa tatttatagg
      841 ctaaagtaat aagattacat tattagatac tgtataagtt ttttaaatct gtaaaaataa
      901 atacacttat aagaattaaa attgctatga aactagtt
//
LOCUS       DAYASV010000292          918 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYASV010000292 DAYASV010000000
VERSION     DAYASV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.297"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            298..705
                     /gene="tnpA"
                     /locus_tag="ACGI68_003837"
     CDS             298..705
                     /gene="tnpA"
                     /locus_tag="ACGI68_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045009167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFH0140207.1"
                     /translation="MEVTHGRGYVYLVQYHIVWCVKYRHPVLTNEIEEDLINILNKIA
                     EDNKFQILECNSDKDHIHLLINCSPQHYIPDIIKALKGVSARLLMKKHGEQLKKKLWG
                     GHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            707..>918
                     /locus_tag="ACGI68_003838"
     CDS             707..>918
                     /locus_tag="ACGI68_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140208.1"
                     /translation="MAVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNNKETFTYKQCSSDLTNLKKELKWL"
BASE COUNT          389 a           97 c          164 g          268 t
ORIGIN      
        1 aaacttcaac gactattcct cttgagggaa gtacactaca agctatcggt agtggaagtg
       61 ggtagacctt aacggataat gccgaaggat aagatatagt ctgtgcttgc atgaaagtgt
      121 aagaagttca taagagaact gcataggtag tagcgaactt atgtgaacga caacctcaaa
      181 aatgataaaa gttaagattt gcgtttctta cgtatgttat ttaaaaataa atataaaaac
      241 cttaagaata cttgttttaa gtaattacta tgttaatatt atgttgggtg atgaaaaatg
      301 gaagtgactc atggtagagg gtatgtgtat ttagtacaat accatatagt atggtgtgta
      361 aaatatagac atccagtatt aacaaatgaa attgaagaag atttaattaa tatattaaac
      421 aaaatagctg aagataataa atttcaaata ttagaatgta atagtgataa agatcacata
      481 catttactaa taaattgttc accacaacac tatataccag atataataaa agcattaaaa
      541 ggtgtatcag caaggctgtt aatgaaaaaa catggagaac agttgaaaaa gaaattatgg
      601 ggaggacatc tttggaatcc tagttatttc gttgctactg tatcagaaaa tacagaagaa
      661 cagataagaa aatatattaa taatcagaaa aaacaagtaa agtgaggtgg cagtagtgga
      721 aaaagcttat aagtttagaa tgtatccaaa taaaaaacaa caagagttaa ttaacaaaac
      781 ttttggatgt tgtagatttg tatataataa gtatcttgct aaaagaatag aagtttataa
      841 aaacaataaa gaaactttta cttataaaca gtgtagttct gatttaacca atttaaaaaa
      901 agaattaaaa tggcttaa
//
LOCUS       DAYASV010000293          905 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYASV010000293 DAYASV010000000
VERSION     DAYASV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.298"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..858
                     /locus_tag="ACGI68_003839"
     CDS             <1..858
                     /locus_tag="ACGI68_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFH0140209.1"
                     /translation="LLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGLG
                     VVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMATL
                     DITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIVD
                     CVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKKI
                     MEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHEA
                     LGNGFKKIE"
BASE COUNT          327 a          104 c          143 g          331 t
ORIGIN      
        1 ttattgaaaa aagatagaat aactttttca gaaattttaa tattaattgt aggctgtata
       61 ttaatggcca tttctcttaa cttatttttt aatccacatg caatcgctgc tggtggtata
      121 acaggacttg gagttgtttt aaattcatta ttcggtgtag aactttggat tgtcaattta
      181 cttttaaatg ttccattatt catatttgct tacaaaatac tttcaaaaaa agattgtttt
      241 aaaacggttt taggaataat ttttcttacc atagcactta agcttactgc aaatatggct
      301 acattagata taacaaatga tatgtatctt gcaattatat ctggttctat tttaatggga
      361 gttggtcaag gacttatttt tagaataaat ggctctacag gtggaactga tttaatggca
      421 ctcctactaa ataaatattt tcctacattt agtatacctg ttttaatggg tattgtagac
      481 tgcgttgtag ttgttttatc aggtatagta aatagacagg ttgaaattgc actttattca
      541 actgtggctt tatacatttt agtaaaagtt agtgacttgc ttattgaagg atttaattat
      601 tccaaatcat tcactattat atctgacctt tcaaaagata taagtaaaaa aataatggaa
      661 gatttagata gaggtgcaac tattctaaaa ggagaaggtg catatactgg agaaaataaa
      721 aatgtacttt tagtagtagt tgaaaagaaa gaagttgtcg aattgaaaaa attagtaaaa
      781 aatgtcgacc ctaatgcttt tattataatt acagatatac atgaagcttt aggtaatgga
      841 tttaaaaaaa tagaataact aacaaataaa tagcaagtat aattaacata tacaaattca
      901 acttt
//
LOCUS       DAYASV010000294          835 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYASV010000294 DAYASV010000000
VERSION     DAYASV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.299"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(400..606)
                     /locus_tag="ACGI68_003840"
     CDS             complement(400..606)
                     /locus_tag="ACGI68_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140210.1"
                     /translation="MNQSQEKDLEFIKNHWDLTIIEKERNVTRIIRYSLIILAIIAFF
                     LPTKIKFSIYIFISLFCWLMYLVL"
BASE COUNT          327 a           77 c          101 g          330 t
ORIGIN      
        1 ctctactaat tttaaacatt tttaaataaa tatttgtata taccttatat aatattatag
       61 taattagttt taaatcaata gaatagtatg tatttatcta atgcggcaat ataatttaaa
      121 gctggtgttg tagaaaaact aagagtgaat atagcaaaga gaagcaatag tattttaaac
      181 tttgtctctc ttattttttg aaaactaaca ttaaaacata aggtataaat aatattatca
      241 tataaatgaa aacaaatttg aatttttctc tggtcgatgt atatagtaag tttgttgata
      301 tgagaaaaat tattattgat aatatacatg catagaaagg aaatggtata taatattttc
      361 ttaagtttct tttttaggca tttctattgg cattttaggc tatagtacta aatacattaa
      421 ccaacaaaat aatgaaataa atatatatat agaaaactta attttcgtgg gaagaaaaaa
      481 agcaataata gcaagtatta ttagagaata acgaataata cgagtaacat ttctttcttt
      541 ttctatgata gttaaatccc agtgattttt tataaattca aggtcttttt cttgactctg
      601 attcatttaa tcatctcctg ttaatagtat aattttaaag ctattacttt gttttatttt
      661 aacatattta tacactaaag tgaattttat gattagattt atttaattat ttctgaatta
      721 aaacttatta gaattctcta ttttgataat ttcaaactct attgattgta tgataattga
      781 aactatagga tatgtaggat aacaagtttc aaagtagtta aaataaaaaa gataa
//
LOCUS       DAYASV010000295          787 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.300, whole genome shotgun sequence.
ACCESSION   DAYASV010000295 DAYASV010000000
VERSION     DAYASV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.300"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>787)
                     /locus_tag="ACGI68_003841"
     rRNA            complement(<1..>787)
                     /locus_tag="ACGI68_003841"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAYASV010000296          753 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.301, whole genome shotgun sequence.
ACCESSION   DAYASV010000296 DAYASV010000000
VERSION     DAYASV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.301"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..>753
                     /locus_tag="ACGI68_003842"
     rRNA            <1..>753
                     /locus_tag="ACGI68_003842"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          207 a          156 c          227 g          163 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg catgagtagc gagatgtggg tgagaatccc acaggccgta aacccaaggt
      661 ttccagggga aggttcgtcc gccctgggtt agtcgggacc taagctgagg ccgaaaggcg
      721 taggtgatgg acaacaggtt gatattcctg tac
//
LOCUS       DAYASV010000297          708 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.302, whole genome shotgun sequence.
ACCESSION   DAYASV010000297 DAYASV010000000
VERSION     DAYASV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.302"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            109..>708
                     /locus_tag="ACGI68_003843"
     CDS             109..>708
                     /locus_tag="ACGI68_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFH0140211.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSN"
BASE COUNT          249 a           56 c           91 g          312 t
ORIGIN      
        1 taaataatta tattaaattt aagtgtgttt tttatgctat aattagtatg tattttttat
       61 gatatatttt ataaaaaata taatttaatc atttttgagg atattattat gttagatgca
      121 tttataacaa ttgtttcttc tattatagaa atatggtcat gtaaaaaagt ttttgattat
      181 actagtcagt caaagaccga cttaaaaaaa ctaaatatgt attttctctt ttctgttttt
      241 ctcattattt cttctttata tataaatata ggagctaata gtagaatctt gatatgtata
      301 tttgtaatgt tcgttttcta taagtctaac tataaggtta atatatataa atgtctggta
      361 atctcatttt tttattggtt aatattaata ggtataaatg gattaagtat gttattagta
      421 gcaacagtaa gcaatttgga taatatgaat gttttactta gtggcagttt atatagatat
      481 gaagcaatat tcttgggaaa gataatgtta ggtatattac tttatgttta taaggcttct
      541 tttaaaaatt ttaaaataag taaaaaagag atatacatat gtattcctat gatagcaaat
      601 ttattgtcat tttttgtact ttatgattat attattaatc ttgtaaaaat ctctcctgaa
      661 agaagaactg cttttttagt aatagtttta ctattaatat tatctaac
//
LOCUS       DAYASV010000298          688 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.303, whole genome shotgun sequence.
ACCESSION   DAYASV010000298 DAYASV010000000
VERSION     DAYASV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.303"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            88..>688
                     /locus_tag="ACGI68_003844"
     CDS             88..>688
                     /locus_tag="ACGI68_003844"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFH0140212.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNF"
BASE COUNT          286 a           82 c           87 g          233 t
ORIGIN      
        1 tatcttataa ttagataaat cataattata tattattgtt aaaactcaat tatataattt
       61 ttaataatat tattttagga ggtaaaaatg ataaacgata ttaaaaataa attcaaaaat
      121 tacaaaccat atataaatgg atacaagaat atgaagagag catctgtttt aattccaata
      181 gtagaaataa ataacacaca ctatatatta tttgaagttc gctcaaaaaa tttgaagcat
      241 caacctagcg aaatttcttt tccaggtggt aaaatagaat caggtgagac tccatatgaa
      301 gctgttatta gagaaacttg tgaggaactt ggaactttta gtgacaatat agaagtaata
      361 tcaagtcttg acctccttat aacaccagta aagtttatca tacatccata cttaggatat
      421 ataaaaaata ttaataattt aaatataaac aaggatgaag ttgaccatac ttttttggtt
      481 cctataaaat atcttttaga gaatcctcca aatacttata gtaatactgt aaaagttttt
      541 ccagatgaaa atctaccata tggttcaatt ccaaaccaaa aagattataa atttgaaagt
      601 ggaagttatc ctattctgtt ttataaatat aaagattatg taatttgggg aatgacagca
      661 aaaatactag aaaacttttt aaattttt
//
LOCUS       DAYASV010000299          685 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.304, whole genome shotgun sequence.
ACCESSION   DAYASV010000299 DAYASV010000000
VERSION     DAYASV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.304"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(142..621)
                     /locus_tag="ACGI68_003845"
     CDS             complement(142..621)
                     /locus_tag="ACGI68_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFH0140213.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVAGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          269 a          106 c           53 g          257 t
ORIGIN      
        1 aaacataatt atattttagt attataatct tttaatttta gcattatagt ttatgtatta
       61 taccctaata tattttattt taaattacta gttagtcact cttttcgggt tatttatctc
      121 ctatatatta aagtgtcaca tctaaatagt taaatcatta tcctctttta tctttattgc
      181 ctttacaaat ccatctgcaa ttacaaaact cataagagaa gttataaaat atacaaatac
      241 tggaacagta aaataaaatc caaatcctaa atctagaaat ctcattcctt ttccagtagt
      301 accaaaagtt gaaataaact caattagaga gtttaaaagc aaaagatatc caataatcct
      361 aaatcttttt acattctctt ttacaaatag gctatattca gaactaccta atacttctat
      421 caatgatttt ataatgataa aatatgatac acctaaaata aacattaata ttccataaat
      481 agtaccttgt agagttgctt ctccactaaa acagaggtac ataaaacttt taaatcctcc
      541 tgcaacaatt actaatccta aaagaaaaaa taaaatttgt aatataccac ttagtaaatt
      601 ttttataagt ttattagaca tcatgccaac tcctttaaaa tattttatat atagtataat
      661 acattatatt atatcgtttt tcaat
//
LOCUS       DAYASV010000300          654 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.305, whole genome shotgun sequence.
ACCESSION   DAYASV010000300 DAYASV010000000
VERSION     DAYASV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.305"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..161)
                     /locus_tag="ACGI68_003846"
     CDS             complement(<1..161)
                     /locus_tag="ACGI68_003846"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140214.1"
                     /translation="MFFSLFKKKSGYTFTNFLSKVRVEKSKKYILKKDLSILDVAVLI
                     GFNSQNYYS"
     gene            454..645
                     /locus_tag="ACGI68_003847"
     CDS             454..645
                     /locus_tag="ACGI68_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140215.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
BASE COUNT          266 a           59 c           85 g          244 t
ORIGIN      
        1 atactatagt aattttgact attgaatcca attagtacag caacgtctaa aatagacaag
       61 tcttttttta atatatactt tttgcttttt tcaactctaa ctttacttaa aaaattagta
      121 aaagtatatc cacttttttt cttaaataaa gaaaaaaaca acttttatta agttgtaatt
      181 cattacatat attacttact gttatatctt ctgagtaatt tttgtgaata taatctatag
      241 ttttctttaa gttttcttta cataaaacta agagttggtc tatgtttcat tttatcaagg
      301 catatttatt aaaaaatcaa tctctatttg ttaaaataat ttatgaatga tgttagaaac
      361 tgtaatatat tggatataat ataataaaca gataaactct gtattaagag gatatatagt
      421 ttatcttaat tataggggaa gtgaggatat attatggaaa gattaatgga actcatagaa
      481 agaaaatttt taaaggatgt agaactttta gaattagaag acatagaaaa tgtaatagaa
      541 tgcaaaatga cacagccata taatgctcat tataatgaat atttagtaaa agtaaaaaat
      601 ggagatgaag tagaagaata ttttgtgtat ttaaaacata gataaacata aaag
//
LOCUS       DAYASV010000301          638 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.306, whole genome shotgun sequence.
ACCESSION   DAYASV010000301 DAYASV010000000
VERSION     DAYASV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.306"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..254)
                     /locus_tag="ACGI68_003848"
     CDS             complement(<1..254)
                     /locus_tag="ACGI68_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140216.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          250 a          104 c           78 g          206 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt tttaaaattt
      301 ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtata
//
LOCUS       DAYASV010000302          594 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.307, whole genome shotgun sequence.
ACCESSION   DAYASV010000302 DAYASV010000000
VERSION     DAYASV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.307"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..>594
                     /locus_tag="ACGI68_003849"
     CDS             <1..>594
                     /locus_tag="ACGI68_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFH0140217.1"
                     /translation="RDKQVPQGRILNATISKEPSGRYYVSLCCTNVDIEVFENTNNQI
                     GLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARL
                     QEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNMIRNRKLSRLISDVSWSEFVRQL
                     KYKANWHGRQIVKVGKFFASSQICNKCGYKNEEMKDL"
BASE COUNT          249 a           64 c          102 g          179 t
ORIGIN      
        1 taagggataa acaagtacct caaggtagaa tacttaatgc tactatatct aaagaaccaa
       61 gtggtagata ttatgtatca ttatgctgta ctaatgtaga tattgaagta tttgaaaata
      121 ctaataatca gataggttta gatttaggaa ttaaagaatt ttgtatctca agttgtggag
      181 actttataga aaatcctaag tatcttaaga agtcattaaa caaacttgct aaattacaaa
      241 gagaattatc gagaaagaca atcggtagtt taaatagaaa taaagcaaga ttaaaagttg
      301 caagacttca agaacatata gctaatcaaa gaaatgattt tctacaaaaa ctatctacta
      361 aattaataaa agagaatgat attatttgca tagaagattt acaagtaaag aatatgatta
      421 gaaatcgtaa actatctcgc ttaatttctg atgtgtcatg gtcagaattt gttcgtcaat
      481 taaaatacaa ggctaattgg catggtagac aaattgtaaa ggtaggtaaa ttctttgcaa
      541 gttcacaaat atgcaataaa tgtgggtata aaaacgagga aatgaaggac ttaa
//
LOCUS       DAYASV010000303          577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.308, whole genome shotgun sequence.
ACCESSION   DAYASV010000303 DAYASV010000000
VERSION     DAYASV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.308"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..>577
                     /locus_tag="ACGI68_003850"
     rRNA            <1..>577
                     /locus_tag="ACGI68_003850"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAYASV010000304          503 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.309, whole genome shotgun sequence.
ACCESSION   DAYASV010000304 DAYASV010000000
VERSION     DAYASV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.309"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(45..485)
                     /locus_tag="ACGI68_003851"
     CDS             complement(45..485)
                     /locus_tag="ACGI68_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HFH0140218.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKDINDTLSNIS"
BASE COUNT          173 a           62 c           47 g          221 t
ORIGIN      
        1 ataaataatt gtaactagat aatattcaat ttctatatta atatttaact aatattagat
       61 aatgtatcgt ttatatcctt ttctactaat ggtttcatat tattattata aatacttata
      121 tctgcattac ttaattccat ttttatgcga ttaataagat tttctttata ctttgtaata
      181 ttaggtatgg atttaataaa cattcttgat acagaaggtt ttttatctac gatatgactt
      241 agtatactat ctatatttat ttcaatttta ttatcaatat cccactgagc atttgcagag
      301 attaataaca atccccttgt tcgaatatat gagttttcgt tatcaatcat ttctaggaat
      361 gagtcaaaaa aacaatatgt aatatttgat tttaaacttt cattttctaa gaataaaaaa
      421 gttttatatg cttcattatg attttttgaa tataatttat taataagtaa attgagattc
      481 ttcaatcttc tcatctccct cat
//
LOCUS       DAYASV010000305          496 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.311, whole genome shotgun sequence.
ACCESSION   DAYASV010000305 DAYASV010000000
VERSION     DAYASV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.311"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..>496
                     /locus_tag="ACGI68_003852"
     CDS             <1..>496
                     /locus_tag="ACGI68_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFH0140219.1"
                     /translation="GTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRKLLD
                     QAQAGDNIGALLRGVQRNEIERGQVLAKTGSVKAHTKFTAEVYVLKKEEGGRHTPFFD
                     GYRPQFYFRTTDVTGACKLPEGIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRT
                     VASGV"
BASE COUNT          213 a           61 c          125 g           97 t
ORIGIN      
        1 ggaacagttg caacaggaag agtggaaaga ggagtactaa aagtacaaga cgaagtagaa
       61 ttagtaggat taacagaagc accaagaaaa gtagtagtaa caggagtaga gatgttcaga
      121 aaattattag accaagcaca agcaggggat aatataggag cattattaag aggagtacaa
      181 agaaacgaga tagaaagagg acaagtacta gcaaagactg gatcagtaaa ggcacacaca
      241 aagtttacag cagaagtata tgtacttaaa aaagaagaag gtggaagaca tacaccattc
      301 tttgatggat atagaccaca attctatttc agaacaacag acgtaacagg agcttgtaag
      361 ttaccagaag gaatagagat ggtaatgcct ggagataacg taacaatgga agtagactta
      421 ataaactcaa tagttgtaga agagggatta agattctcaa taagagaagg tggaagaaca
      481 gtagcttcag gagttg
//
LOCUS       DAYASV010000306          493 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.312, whole genome shotgun sequence.
ACCESSION   DAYASV010000306 DAYASV010000000
VERSION     DAYASV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.312"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="ACGI68_003853"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="ACGI68_003853"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="ACGI68_003854"
     tRNA            310..398
                     /locus_tag="ACGI68_003854"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
     gene            410..486
                     /locus_tag="ACGI68_003855"
     tRNA            410..486
                     /locus_tag="ACGI68_003855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Ile,seq:cat)
BASE COUNT          156 a           91 c          109 g          137 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgaa tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttggtt agagcgcccg gctcataacc ggtaggtctg gggttcgagt ccctgatggc
      481 ccaccacaat aat
//
LOCUS       DAYASV010000307          484 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.313, whole genome shotgun sequence.
ACCESSION   DAYASV010000307 DAYASV010000000
VERSION     DAYASV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.313"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>484)
                     /locus_tag="ACGI68_003856"
     rRNA            complement(<1..>484)
                     /locus_tag="ACGI68_003856"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          115 a          143 c          108 g          118 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaag ggattagctc caccttacgg cttggcaacc ctctgtacta cccattgtag
      301 cacgtgtgta gccctaagca taaggggcat gatgatttga cgtcatcccc accttcctcc
      361 aggttatccc tggcagtctc tctagagtgc ccaacttaat gatggcaact aaagacaagg
      421 gttgcgctcg ttgcgggact taacccaaca tctcacgaca cgagctgacg acaaccatgc
      481 acca
//
LOCUS       DAYASV010000308          438 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.314, whole genome shotgun sequence.
ACCESSION   DAYASV010000308 DAYASV010000000
VERSION     DAYASV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.314"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
BASE COUNT          110 a          168 c          110 g           50 t
ORIGIN      
        1 gccaaaatca cctgcccgct acaccagcac cacttcgggg acaagccgga atcacgtgag
       61 gggcatgccg gcacagcctc tcagactgaa cctcgtgccg gacaccacag aaccacctgc
      121 cggacagacc ggaaccacct gccggacacg ccggaaccac cggctcgacc cgccggaacg
      181 accgctggac atgcaaaatc acctgccggc tacgccggaa acacttggcg aacactccgg
      241 aatcacctgc cgggcatgcc ggaaccacct ctcagatatg ccagaaccac gtgccaatga
      301 ccccggaagc acctgccgag cacgccggaa ccacctgctc gactcgccag aaccacctgc
      361 cggacacgca aaaatcacct gccggctacg ccacaaccag ttggggaaca cttcggaatc
      421 acctgccggg cttgctgg
//
LOCUS       DAYASV010000309          374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.315, whole genome shotgun sequence.
ACCESSION   DAYASV010000309 DAYASV010000000
VERSION     DAYASV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.315"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..372)
                     /locus_tag="ACGI68_003857"
     rRNA            complement(<1..372)
                     /locus_tag="ACGI68_003857"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a          102 c           63 g          122 t
ORIGIN      
        1 aggactatta ccttctgtgg tgggtctttc caaacctctt caattacgat acctcttcgt
       61 tatgatatgt ccgcaacccc aataaagaaa actttattgg tttgggctaa tccgcgttcg
      121 ctcgccgcta cttacggaat cgaatttctt tctcttcctt caggtactta gatgtttcag
      181 ttccctgagt tcccctcatt aagctatgta ttcacttaat gatacttaga cattactcta
      241 agtgagtttc ctcattcgga aatcttcgga tcaaagttta cgtgcaactc cccgaagctt
      301 atcgcagctt atcacgtcct tcatcggctc ctagtgccaa ggcatccgcc ctgcaccctt
      361 aataacttga ccag
//
LOCUS       DAYASV010000310          367 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.316, whole genome shotgun sequence.
ACCESSION   DAYASV010000310 DAYASV010000000
VERSION     DAYASV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.316"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
BASE COUNT          147 a           24 c           52 g          144 t
ORIGIN      
        1 gttatataga tttaatataa ataatgtatt ttttgtagat gatttatgta agatttatga
       61 ccaatttgtg tatgatttgt gttctttaat catgacaata ttatattgta aaaaataaat
      121 ttaataaaaa atgacttgga atttatattc taggtctttt ttatataaaa atttaataag
      181 tttcaaatgt tggtaacttt gacagtagaa aacgtctatt gagataacga ggattctaat
      241 tgaggtggag agatattagc acttaaagta gtatatgcaa cagatataat ctttacaaaa
      301 gtaatttgaa ttaaaatgta aatataaaga ataattaaaa aaatatcgca attatttgtt
      361 tttcata
//
LOCUS       DAYASV010000311          363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.317, whole genome shotgun sequence.
ACCESSION   DAYASV010000311 DAYASV010000000
VERSION     DAYASV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.317"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            167..283
                     /gene="rrf"
                     /locus_tag="ACGI68_003858"
     rRNA            167..283
                     /gene="rrf"
                     /locus_tag="ACGI68_003858"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            290..361
                     /locus_tag="ACGI68_003859"
     tRNA            290..361
                     /locus_tag="ACGI68_003859"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Asn,seq:gtt)
BASE COUNT          116 a           61 c           78 g          108 t
ORIGIN      
        1 ttcattaaga tatatgtgca gtttttagag tgttaactct atgtaaagta ctaacttcaa
       61 ttgatgaagt atatatcgta aaaacaatta ctatttagta cttcaaaact attgacattt
      121 aaaaataaag atgttaatat aatacttgtc ttataaatag gcaatatgtg gttataatag
      181 caaagaggat acacctgttc ccattccgaa cacagaagtt aagctcttta gcgctgatgg
      241 tacttggtgg gaaactgcct gggagagtag gacgtagcca cgtgaatatt ccaaggtagc
      301 tcaatggtgg agcaaccggc tgttaaccgg taggctgtgg gttcgagccc cacccttgga
      361 gcc
//
LOCUS       DAYASV010000312          337 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.319, whole genome shotgun sequence.
ACCESSION   DAYASV010000312 DAYASV010000000
VERSION     DAYASV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.319"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     misc_binding    106..317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          133 a           29 c           57 g          118 t
ORIGIN      
        1 aatttacata taaaactaaa aaatgcaagt aattgacttt ttaagttgac aaaatattgc
       61 atatatatta tgatataaat cataaattaa ctacaaatca atattatata ttcgtagaag
      121 aagaaagtag cataaaggaa ttttacagag agttagattt ggctgagaac taatattgga
      181 atttatgtga aaaggacttt ggagaatact attgtaatta tgttagaata gtggtttaat
      241 tcgccttaag aaaatgagtg agtaaaatta atttttacta ataagagtgg taacacggat
      301 ttatattcgt ctcttggatt tttccaagag gcgtttt
//
LOCUS       DAYASV010000313          232 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.324, whole genome shotgun sequence.
ACCESSION   DAYASV010000313 DAYASV010000000
VERSION     DAYASV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.324"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>232)
                     /locus_tag="ACGI68_003860"
     CDS             complement(<1..>232)
                     /locus_tag="ACGI68_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HFH0140220.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPE"
BASE COUNT           66 a           41 c           37 g           88 t
ORIGIN      
        1 aattctgggt cttctttcat catttcatct aataaagctt gtattggagc taatacgcct
       61 tctcttgttt ctccaactaa taatcttcta tcaaaagtag ctttgcaata atctggcact
      121 actgatgctc ctggatatgg agaagatttt atatctgtta aaactaatat tccatcacct
      181 aaaactggat gagttggagg tactaattgt tgtatctttt ggattacatt ag
//
LOCUS       DAYASV010000314          219 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.326, whole genome shotgun sequence.
ACCESSION   DAYASV010000314 DAYASV010000000
VERSION     DAYASV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.326"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>219)
                     /locus_tag="ACGI68_003861"
     rRNA            complement(<1..>219)
                     /locus_tag="ACGI68_003861"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           42 a           64 c           47 g           66 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtccc
//
LOCUS       DAYASV010000315          217 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.328, whole genome shotgun sequence.
ACCESSION   DAYASV010000315 DAYASV010000000
VERSION     DAYASV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.328"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
BASE COUNT           73 a           14 c           32 g           98 t
ORIGIN      
        1 attatggtat agtgtttttt aattttaaga aaaaatataa tgacatccta aaaaagtttt
       61 aattataagc tgttctgtgt agttataata gcttctatgt aactattgta agtatatttt
      121 attatagtta tttatttaaa gtacgataaa tgttttaaat tgaagtattt tgcgccattg
      181 agttctacta aagtagagtt tttgtgtagt attttta
//
LOCUS       DAYASV010000316          213 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.329, whole genome shotgun sequence.
ACCESSION   DAYASV010000316 DAYASV010000000
VERSION     DAYASV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.329"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..>213)
                     /locus_tag="ACGI68_003862"
     CDS             complement(<1..>213)
                     /locus_tag="ACGI68_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140221.1"
                     /translation="SLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIKDTTA
                     IIVGSFIYQFTIYFAMSLGMLSTDLKL"
BASE COUNT           83 a           33 c           31 g           66 t
ORIGIN      
        1 tagttttaag tcagttgata acattcctaa actcattgca aagtatatag tgaattggta
       61 tataaaagag ccaacaatta ttgcagtagt atctttgata aaagtaaatt tcttaaatag
      121 tgtaatacca attatgatag aggcaattcc aagaactaaa gttcctatac ccatatttac
      181 atctgagaat cccaagaact gagccataag act
//
LOCUS       DAYASV010000317          209 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.330, whole genome shotgun sequence.
ACCESSION   DAYASV010000317 DAYASV010000000
VERSION     DAYASV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.330"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
BASE COUNT           91 a           25 c           32 g           61 t
ORIGIN      
        1 taaatatttc actaaaaaaa aacaaatgca aagctagtac caaaaataaa tcagaaaaaa
       61 gaggtgttgt agatgttaca aattaaaaat ctttcaaaga gtttccctaa tccatatggt
      121 gaacctaata ctatctttga aaatttatca atcgatatag aagatggtga atttgtaagt
      181 atcataggta gtaatggaac tggtaaatc
//
LOCUS       DAYASV010000318          206 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.denovo.331, whole genome shotgun sequence.
ACCESSION   DAYASV010000318 DAYASV010000000
VERSION     DAYASV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.denovo.331"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(<1..167)
                     /locus_tag="ACGI68_003863"
     rRNA            complement(<1..167)
                     /locus_tag="ACGI68_003863"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAYASV010000319        17165 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.guided.087, whole genome shotgun sequence.
ACCESSION   DAYASV010000319 DAYASV010000000
VERSION     DAYASV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.guided.087"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            complement(218..742)
                     /locus_tag="ACGI68_003864"
     CDS             complement(218..742)
                     /locus_tag="ACGI68_003864"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0140222.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQVMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(807..1559)
                     /locus_tag="ACGI68_003865"
     CDS             complement(807..1559)
                     /locus_tag="ACGI68_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0140223.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1590..2888)
                     /locus_tag="ACGI68_003866"
     CDS             complement(1590..2888)
                     /locus_tag="ACGI68_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HFH0140224.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVESTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEGIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2905..5169)
                     /locus_tag="ACGI68_003867"
     CDS             complement(2905..5169)
                     /locus_tag="ACGI68_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HFH0140225.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5424..6242)
                     /locus_tag="ACGI68_003868"
     CDS             complement(5424..6242)
                     /locus_tag="ACGI68_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFH0140226.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6235..6933)
                     /gene="cbiQ"
                     /locus_tag="ACGI68_003869"
     CDS             complement(6235..6933)
                     /gene="cbiQ"
                     /locus_tag="ACGI68_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HFH0140227.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASINMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6936..7220)
                     /locus_tag="ACGI68_003870"
     CDS             complement(6936..7220)
                     /locus_tag="ACGI68_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HFH0140228.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7210..7962)
                     /locus_tag="ACGI68_003871"
     CDS             complement(7210..7962)
                     /locus_tag="ACGI68_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFH0140229.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8144..8336)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8468..10240)
                     /locus_tag="ACGI68_003872"
     CDS             complement(8468..10240)
                     /locus_tag="ACGI68_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HFH0140230.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDVILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10254..11183)
                     /locus_tag="ACGI68_003873"
     CDS             complement(10254..11183)
                     /locus_tag="ACGI68_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0140231.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11404..11589
                     /locus_tag="ACGI68_003874"
     CDS             11404..11589
                     /locus_tag="ACGI68_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0140232.1"
                     /translation="MKYGCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKNA
                     EKPVDGRTKRRKEVQYE"
     gene            11582..12283
                     /locus_tag="ACGI68_003875"
     CDS             11582..12283
                     /locus_tag="ACGI68_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0140233.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHCGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDCIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12285..13208
                     /locus_tag="ACGI68_003876"
     CDS             12285..13208
                     /locus_tag="ACGI68_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0140234.1"
                     /translation="MNTSVYLLLILVLSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13365..14288
                     /locus_tag="ACGI68_003877"
     CDS             13365..14288
                     /locus_tag="ACGI68_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140235.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHAKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSEYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14281..15018
                     /locus_tag="ACGI68_003878"
     CDS             14281..15018
                     /locus_tag="ACGI68_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140236.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANTALGRRK"
     gene            15029..15802
                     /locus_tag="ACGI68_003879"
     CDS             15029..15802
                     /locus_tag="ACGI68_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140237.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETKLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIISLLLCACLSAFILKGLKHNEKN"
     gene            complement(16343..16951)
                     /locus_tag="ACGI68_003880"
     CDS             complement(16343..16951)
                     /locus_tag="ACGI68_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0140238.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5541 a         2974 c         2164 g         6486 t
ORIGIN      
        1 ttaaaaataa atatataaac aatcataata aaattttttt aataaaactt tattttttag
       61 ttaaaatcat acaatatata gatttccaca atacaaataa attaaaaagt gaaatgtatt
      121 tgtctaaaaa cataataaca tttcacttta tcctcatttt taagtaatat atatttttaa
      181 tttttattta taactaaatc tagtttttat ataaatttta gtttttttct acatctgagt
      241 aatctattcc aaaagtttct acagtaacag atttcataac ttgtggagtt tgtggcttat
      301 cagctgaatc tgttctaact gatgctattt catcaactgt atccatacct tctatcactc
      361 taccaaaacc tgcatactga ccatctaagt gtggtgagtt tttatgcatt atgaaaaatt
      421 gagaaccagc tgaattaggt gccatagttc tagccattga tatgactcct ctttcatgtt
      481 ttaaattgtt agtaaatcca ttaccactaa attcaccttt tattgagtga cctggaccac
      541 ccattcctgt accatttggg catcctcctt gtatcataaa tcctggtatt actctatgaa
      601 atattattcc attgtaatat ccttcattta ctaatgatac aaagttttta actgtatttg
      661 gagctatatt tggatataac tcaattttga tttcttttcc attttccatt tctatagtta
      721 ctataggatt tttattttcc ataatttatt accttccttt tcattagtta aattacataa
      781 ttacatttat tatattatac tctaaattaa agtctatcaa gtatattttt tcctatttca
      841 tatccttctt gataaatagt tttacaaaac tcatcatcaa agttaagcgt gtctttagga
      901 gcaaattgag tattaggtct aactactatt attttatctt catcatatac ttttttaata
      961 tcttctggca actcataatt atattttgtg gatattaaaa taattttatc cacattatcc
     1021 ccaattaatg gttcaattaa tgcactccta attaaacctc catctaattt gaaaccatca
     1081 taaattcctt cttgcacatc tttcacaaat tgttctttaa atccaagagt tgcgtttgga
     1141 tttgatggta acaacccact gtatgaaatg ctgataatgg cttcattacg aggcattttt
     1201 gatacgtcaa caatcttttc tgatactact ttattatcaa cttcaacata attaacataa
     1261 aagttcattt cttctatatt actatcatct agttccctta ggttgtctat agcaggagaa
     1321 ttatccacag tattatttac tatttttaca tttccattct ggatatcttt tatatttgtc
     1381 cacattttct ccaagttatc aacattccct gtgtacaaat aatatccatt tattgctcct
     1441 attgatgtcc ctgaaatcga ataaaattta tttattcctc catcatacaa ggctttaact
     1501 actcctgctt gataagcccc ttttgctcct ccaccttcta aacataatcc taatttcata
     1561 gtctcatctc caaattgttt atcttatatt tatatttcaa aaaacttact aaatgtttta
     1621 tatatactat agtggtcctc aacatttcct aattctctta tagaatgcat tgctaaaatt
     1681 ggacttccca catccacaga tggtatatct atatgagtag aagatattgg acctatagta
     1741 cttcctcctc tttcatctga cctattaaca aattcttgat actcaacatt agcttctcta
     1801 catatatttt tataaatagc tatagaatat ccatcactag tatatgcctg actagcattt
     1861 atctttataa ccggaccttt tcccattatt ggtttattag ttgggtcatg cttttctgga
     1921 atgtttggat gaatagcatg agctaaatct gatgatatca taaaagatga ataaatagac
     1981 tcaaagaatc cctctctatc cttacccatt gatatgcaaa ttctctccaa tatatttaac
     2041 aacatgtttg aatctgcacc ttgtttagta gagctaccaa cttcttcatt gtcaaataca
     2101 gccattacat ttacaccatg ttgaccttta gtattaacaa gagcattcaa actagcatgt
     2161 accattgcaa gattatcttg tctcccaata gatataaatt catcattagc ccctattaaa
     2221 cttccttttt caaattcata taaaaataaa tcaaaatcta taatatcgtc catatttaca
     2281 tctaagttag atgctatttg ctttataaga aaattatctt tttctaatgt atcatttaac
     2341 atcccaacta aaggtaacat atctttttgc ttattatatg agtgaccatc atttatactt
     2401 ctatttaaat gtattgctag atttggtatt atacaaattg gctttttaaa gtctataagc
     2461 ctttcatttg gttttaatat actatcacct tttaaaacta ctctaccagc tatcccaaga
     2521 ggtctatcca accatgtact taatattgct cctccatagc attctgtatt taacttcaaa
     2581 taagattttt cagcttctat ttctgcattt ggtttaatac taaatgtagg agaatctgaa
     2641 tgagagccta ttattctaaa tccatctctt tctatacctt ctgaatttac tacaaaagct
     2701 actatagctg ataaattctt agtaacataa tactttccac caactcttag attccatctt
     2761 tgattcaaaa ctaattcttc aaatccattt ttatctaaga tttccttagt actctcgact
     2821 gcatgaaaag acgacggact atcatatatg aagtctatta agtttttagc aaagtttata
     2881 ctatccattc ctcatttacc tccattataa aatatttagt gttgtatatt ctttatttat
     2941 ttttttaatt ccctgttggt caaaagctat agtaacatct gtaccaatta tagaaaccac
     3001 tgtcccaacc ccaaatttag gatgatgtac cttagaacca agttttatat catctggatt
     3061 agtttcttta ttagaatctt ttattgtagc attaacttta tctgcaactt tagatttgct
     3121 tatagtactc atatactttt tagtatattt atctaacata ttataacttg ctttagaata
     3181 gcttagttct ttttttactt tgtacaatct ttctatacat tcttctggca gttcttccat
     3241 aaatcttgaa gctattgcaa catttgtttt accatataat gttctcttct ctgtcaatga
     3301 catatatagc tcttctttag ctctagttat accaacataa caaagtcttc tctcttcttc
     3361 tatttgtgaa tcactcatag attttatagc tcttgatata ggaaataacc cttcttccat
     3421 acctgttaaa aacacaacag ggaactctaa acctttggat gtatgaattg tcataagcga
     3481 aaccctatca ttgtcttctt catcatttga ttcagatgta agtgctatac ttgttaggaa
     3541 tgtctctaag tctttatttt cataagtatc atcattacta ctttcaaact ccaaagctat
     3601 agaaataaat tctctcaaat tatcaattct gtcttgagct tcttcacctt tgcctgttaa
     3661 atctttttca tttttatttc ttatctctac aagctcgtcc atatatcctg tagtatccaa
     3721 gactttttct ataagtttgc taacaggata aacttctttt atagttctaa gagtccctat
     3781 aatatcaaca aatccactta tacttgccct tgccttagta gatatatctg aatttgtttc
     3841 tatatcaatt aatactgagt atatactttc ctgttttaag ttagctctat cttcaatttt
     3901 ttctatagtt ctaagcccta tacctcttct aggaacattt ataatccttt ttatagatat
     3961 atcatcttgt ggattttgaa taactcttaa atacgccaac aaatccttta tttcttttct
     4021 ctcatagaat tttgtaccac catatatatt gtatggaatt tgacttctat ttaaagcgtc
     4081 ctctacaggt cttgcctgtg catttgctct ataaagtact gcaaaatcct tataaggtct
     4141 attttctttt ctagctattt gtgcaattga atcagcaatg aaatcagcct cttctatttc
     4201 actaccagtt aattgtatct ttataagttc tccttctttt ttttcactcc aaagcttttt
     4261 tctttttctt tctatattat tagatataac tttattagct gcatctaata taacttgtgt
     4321 agacctataa ttttgttcca actttacaac atgtacattg tcataatctt tctcaaattc
     4381 aagtatgttt cttatatctg cacctctcca tccataaata ctttggtcat catctccaac
     4441 aacacaaata ttttgatgtt ctcttgctaa taattttatc aattcatatt gagctttact
     4501 tgtatcttgg tactcatcta ccattatgta tctaaatcta cttctgtaat atgctaacac
     4561 ttcatcatta gccttgaaaa gttctacagt tttaagtatt aaatcatcaa agtcaagtgc
     4621 actatttctt tttaaacggt cttgatatag cgcataaata tctgcaatct taaccattct
     4681 attatctgcc atattgatat ctttaaactg ctttggagta taaagtttgt cttttgctcc
     4741 tgaaattgca cttataactg cttttggttc aaacacttta tcacttaaat ttaattcttt
     4801 tagacagtct ttaaccaatg taacttggtc tgctgaatca taaattacaa aacttctatt
     4861 gtaaccaatc ttatttatat cttttcttag tatacgaaca cagcaagaat ggaatgtact
     4921 tatccacata tccttagttt caggacctat attttgttca actctctctc tcatttcatt
     4981 tgcagctttg tttgtaaatg ttatagctaa tatatttggt gcttgtacac ctttatcttc
     5041 tataagatat gctattcttg ttgttaatac cttagtttta cctgaaccag caccagccag
     5101 tataagtact ggaccttcag tcttttctac tgcttctctt tgagcaggat ttaatgtatc
     5161 taaattcata tttctcctcc tgttaacagc cataaatata ttttaatcta aaatatgaat
     5221 tacttcaaat tataattatg tattatatta atagattatt tccaatatat tacaattaaa
     5281 taacattttt ccatataatt tatttcattg tttaataaaa aaaataacct tatgatttgg
     5341 ggaatcataa ggcaccttta aggggtctag gttaagggtt attatatata aatctatttt
     5401 tttaaaatag gtttagggct taattattta tttgtattaa gttctctttc cttccaatac
     5461 ttatacaaat cttcttcttt cttaaataag ggtaaattca tatttctatg tacttttact
     5521 aaccatgggg attctaggcc agcttgtata atattatttt catttataaa cacattttct
     5581 gcttttcctt catcaatcaa aatcccctta tccaatacat atatgtaatc acatatttca
     5641 tacattaaat tcatatcatg actggatatt actattttta tattattttt atttaatccc
     5701 tttattatat ctacaataga cctagtactt actgggtcta aaccagctgt aggttcatca
     5761 agtaatacaa tttcattttc cattgctata actgatgcta ttgccactct ttttttctgt
     5821 ccatagctaa gaaaatgtac tggcctgtct ataaaatcta ttccattaac agctactaga
     5881 gctttgttta ttctctcttt aattgttttt tcatccatac caatgtttct cattgcaaag
     5941 gctatatcat catatacttt tgaataaaaa atttgttttt ctgggtcttg gaagacaata
     6001 cctacatttt ttctaaggtt gtataaactt ctcttatcat attttaactt ttcttcttta
     6061 aaaagaattt ctccagatgt aggctttaat atacccatta aattcataaa taatgttgat
     6121 tttcctgaac catttgaacc tattatacct ataacatttc cctttgaaaa atccatatta
     6181 atgtttaata gtgcattggt atttttttca tattgatacg ttaaattatt tattttaaac
     6241 atcattatcc cctactatgt gaaatttacc atcatacaat tttatgtcta atgatataca
     6301 catatcatca taccttgtca taagtctttt gtacagcata cttccaagaa tacctaagga
     6361 tttatatgaa gtttttaaat ttatatatcc aaatcttagc tcttgtgact tgtatatttc
     6421 tgaaaattct tctaaaaata tgaatataaa cctatatatt aacattgata actcgacaaa
     6481 agtgtcaggt aaatgaagtt ttttaaaaaa gaatattaat tgattaaaag gtgttgtaag
     6541 tatacaaaaa tacacacaag tcaaacaaga catcgctcta aataacacat gaatagacat
     6601 gttaatagac gcctttgata ttcctacatt aaaactaaaa aattcaaaac tatataataa
     6661 atctgcttta ttaaaagata tatttacaag agttattcct atactaagaa ataaaaagta
     6721 cattggtatt ctaagtaatt ttaaataatt ttttaaatct attccagcca cacaaacaac
     6781 caaaatactc attaaaatca taattccact taaaataaat atattttgta ttaccataga
     6841 agcaatcaga aaaattactc ctatggctac ttttttgttt ggatttactt tactcaatct
     6901 atttgtatat gcatatttat caattataag catgattaac ttctctttcc tttttgaaga
     6961 cctaatatgt atcctattac accagcccct aaagctgctt gtgcagagaa taataaactt
     7021 tctatttctc cacttggtgg ctcatatgga ctgctaaacc atggtttgta atttggattt
     7081 atcttagtaa tttcactttc agcttgacca tctgctcctc catattcagc acctgagttg
     7141 acaagtaatg ggaatataat aagagctact actaataaaa gtaataatat gttagttttt
     7201 gtttttgttt tagcactcat tagctttaac tcctttatca ttattttcta gtatgctttt
     7261 ttctccgttt tctgtaatta aattatatac aattacagta agtatacctt ctgctattgc
     7321 tattggtatt tgagtcataa agaaaattcc taaaaattta attgctgaag ccattacccc
     7381 acctgatggg tctgggaaag ccagtgctaa ttgtattgaa gttattgtat atgtggctaa
     7441 gtctcctatt gctgctgcaa gaaatactgg aatagcatta tttccatctt ttttctttaa
     7501 agccttaaat attaggaatg atacgattgg ccctattatc gccattgaaa aagcattagc
     7561 tcctaaagta gttattcctc catgtgctaa taacaatgct tggaatatta aaactatagt
     7621 tccaagaaca aacattacgc ttggtccaaa cattattgct ccaagaccta caccagtagg
     7681 atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct gataatacaa acacaaatgc
     7741 tccacataaa gctaataata ctttcttttt agggtcttgc tttactattt tacctattga
     7801 ttttaatccg aataagaaaa atggtataaa tataactccc catgctatag accattttac
     7861 tggtaagtaa ccttccatta tatgcattgc atttgatgta actggagtta aaatgattaa
     7921 cataagtgca gctataaccc ctaatttaat attctgtttc atcttagtat ccctctctcc
     7981 ctttctaata attaaaacat aataatacat gtctgattta tttaataaaa taaaaaagcc
     8041 taactcgatg gttaggcttc taattttcca caatctaaat acaaacacat aaaacaaaca
     8101 tatctgtata attattataa gaaactttct ccatccccga agaaattact ttaatatttt
     8161 aggcaggtct cctgacttag cttcatccta ctccttttcc ttcccatgag atataaaaaa
     8221 tatcatcata gtggctaaca aaagtttcgt ctgcatcaca gtagcggggg ctgtaaagga
     8281 atttaacctt tttccctatt aatcttgctt aaagaaccta taatattata tatttaattt
     8341 ttaaattatt cttaaatcat tttctatata aatcattgta taccttttct gtgaaaattt
     8401 ctacattttt tataaatttt attcattttt atatttttat gtatataaat tcttttttgt
     8461 aagtttatta atttctaatt atcttcttta aatacattga aactacatca gaaaatctag
     8521 ttatatctct tgaatatata aaatcatttc catatattaa tctttcagct tcaagttctg
     8581 attcttttcc tgtaaatact cctaatacta atatattttg ctttctagct tttctcactt
     8641 cattggctgt atctctcaaa ccaactatac ccctataagc cttttctcct cttaatgttc
     8701 tctcactact ttttcctatc tttacatcat ttggtcttcc atcgctgaga actatcagga
     8761 ttttattttc ttcatctcta tttattagac cactacatat agattttatt gccaaaccat
     8821 ctctattatt accagcacaa aaatattcaa atatattttc actatttttt atactatcat
     8881 cataatcttt aaatcttttt aatattgtat agtctaaaaa actagaaaat cccatcactc
     8941 tatttggtat gccaacttct gttagagcat ttgcaattat gtaagcttga attgccacat
     9001 ttccctgatt tctactttga gagccacttg catccaataa tatatcaata acgtatttcc
     9061 ctttttcatt ttctatatct ttataaaata ttttattatt attacttcta ccaactctcc
     9121 aagctctatt ggctaatata gtaccacaat cagaatatac tctactttta gcattttctt
     9181 ctattaaaat tcttgacaga ctttctttta atctctttat ttgctgtcta tacattctag
     9241 catgttctct aaattttcct aaattatttt ctttttgtct tgttacatat tttagttgaa
     9301 atacattatt acactcactt ctaagaactc catctgtaaa gtgtattcta caaccttcat
     9361 gaacatttct acaattttgt gtctcaattt ttttaatttc acctttactc aaataagttt
     9421 ttccataata atgttctatt tttgaatata tcttatttgc agtgtcttca tcaacatata
     9481 taactcggtc tgctgaattg ctacatttag aatcatcaaa atcccctaca ccttctacaa
     9541 gcatggatga agatattgaa tctatatcac tttcaataat attttcttct tcatcactat
     9601 aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt tacttctaca ctttctacat
     9661 tattttcatc aataagacta ttttcttctc catttaatat gtattcaaaa taagttaaat
     9721 atataacatc taatgctttt aaaatctcca taatatcttg attggtatct atatttctta
     9781 tatcattaac tattcttcta gtcatactat ctaatgctgg gtgcttgtcc atactataaa
     9841 gtacaaatgt gtatttaatt ttatccataa tattatcatt gtgtattttg gtaaaacgtg
     9901 aaacaatatc tttataagca ttccttctta tgtcttctat ccctggcctg tctttaatga
     9961 ccttttcttc aactattgta tttaatataa cttgaattag acctagtata acatctttgt
    10021 tgtatccttt tcttattcta tttgtaagat attttttaac tatatcccaa tctacatact
    10081 ttcttctagc acctgcaatt attgcgaaat atagacctat ttcttttgaa gagtaatctt
    10141 tttctgatat atctatactt tcattgtaat caccagctat tgtccatgat aggttattag
    10201 ctctattttc aaattcataa tcatcatata tccatttcat aatctcacct atattaaata
    10261 tgacttattg gaaacacatc tatactctcc catttattag gtattctagt tttaattaca
    10321 tctccaacca tttctttctc atatacatca aaagtctttc ctgtaagtcc catattaatt
    10381 gcaagagttg gttttaatcc tcttcttata gtcttaagag atgccataag accacgtaaa
    10441 tcaatagctt tactagatat ctctccattt tgagacttca attgtaaatc taaaaatatt
    10501 cctgcaaagt gtattaattt ttcttcatca gcatctgaaa attcattttt taaaatcatc
    10561 attaacttat cttcttctat aggaggaata tctattacca taaatctaga taccagtgct
    10621 tcattaagtt cttttgttcc tgcatattca taattcatag tcccaataaa tcttgttgct
    10681 ggatgtagat tgactctctc atacccaggt acatcaatta ttcttctata gtctaaagct
    10741 gaatgtacca caactatagc atcatttttt gccatattaa tttcatcaaa caccccaaaa
    10801 cctccattaa tagcacactc ataaactgag cctcttctaa gtttaacttc attgtctata
    10861 aatgtatctg tacctataag tgttgaactg tctgtattta tatgaaatga tgtattccac
    10921 tgaggtctat taaatatttc ccctagatta tcagcaagta agttttttcc tgttgccttt
    10981 ggtcccgata ataatatatt ttcttcttct aaaatagctg ctatacacat agacaagata
    11041 tctttaccat aaaaataagt tttagacttt gttactctgt attctaaatc tttttctaaa
    11101 ccataataat ttctaaaata cagtacatct tcaataagtt tttcatctac cccttgcacc
    11161 cttagattat ctattatatc catattttta ccccttttat ataaatagta tttgtttcat
    11221 ttcttatcac cattttagca taaaaggata taagctaaaa ttgatttttt aatctcccca
    11281 ttttaagtaa aaccatgcac cattttatca ctacaaaatt aagagtacta cttactgata
    11341 tttcttgatt ttgttcgtct atacgtatat aatatatgct gataaattat gaggaaagga
    11401 tgattgaaat atggatgccc agaagtagca aaaaaatggg gtatttctga aagaagagta
    11461 caaaaactat gtgaagaaag tcgcatacca ggagtttcaa aactcgggta tatgtggctg
    11521 ataccaaaga acgcagaaaa gcctgttgat ggtagaacaa aaagaagaaa ggaagtacaa
    11581 tatgaatagc gttttgatta tagatgatga caaagaactt tgtgctctta tgaaaaaatg
    11641 tgttgagcaa gaaaatttat ctgctattac tgcacattgt ggtgcagaag gtctacattt
    11701 ggtcgataaa aatagacaca cactctctct tgtaattttg gacataatga tgccagatat
    11761 agacggtttt caagttctga aaaaaattag agaaacaagt aatattccag taattatgct
    11821 tactgctaaa aatgacgagg atgataaagt ttccggacta cgtttaggtg ctgacgatta
    11881 cctaacaaaa ccgttcagca taaacgaact tatggctcgt gtcaattctc ttattcgtcg
    11941 gtatactatg ttaaaccctg cttttataac agacacagat tgcataataa taaatggcat
    12001 gattattgac aagacaaacc gaattgtttc tgtcaataat attcctgttc aacttacaag
    12061 caaagaattt gatttacttg cttttctggc ttctaataag ggacgggtgt ttaccaaaaa
    12121 acagatttat acacaagtat gggaagaaga atatgctttt gacgatagta acatcatgtc
    12181 tttcatcagt aaattgagaa aaaagattga accagacccc aaccatccat tttatatttt
    12241 gacagttcgt ggtgtcggtt atcgttttaa taaggaggct taacatgaat acaagtgtct
    12301 atctgctttt gatattggtt cttagcctga ttattatagc gtaccttgtt tctgtcctgc
    12361 tacgtgtccg aatacaactt acacttataa aagatgcttt agaggatata aaaaatggaa
    12421 accttaatcg acgtatttta acagatgaaa acgacattac aaaacaaatt tgctatgata
    12481 ttaacgaaat agcaatcaat agccaatttc aacttattca gcaaaaacaa tcagaacaag
    12541 cctacaaaca gctaatgaca agtctctccc atgatgtaaa aactccactt gcttctttgg
    12601 tcggttattt ggaagcagta gaaagtaaaa ttgttgtagg agaggaaaaa gacgagtata
    12661 tccatgttgc gtctaataag gctcattact taaagaattt tgtagaaaat ctatttgaat
    12721 gggtaaaatt ggattctaag gaacaagttt ttcattttga tatttttgac ttgaatgaat
    12781 tatcccgtaa tattatatct gattggatac ctgttttaga aagcagtcat tttgaatatg
    12841 aatttgatat accagaaata gagtacttct tacgtataga cgcaaatgca tatacccgta
    12901 tcattaataa cctattacaa aatattatta cccatagtag tggcaacaaa atgacattga
    12961 gaatttttga aaacaaagag caagctcaaa ttataataac agataatggg aaaggtatat
    13021 cttcagacaa tctcccacat atttttgaaa ggttgtacca atgcgaccat tcacgagctt
    13081 caaagggaaa tggtttaggc ttggcgattg caaaggaact tattaacgct cataagggaa
    13141 atattaccgc caacagcact cccggtatgg gaacagaatt tacaatattg cttcctaaag
    13201 ccctgtaaaa gtacgaggct ttcttactct aaaacaagat aaaagcaaga ttatggcaag
    13261 gttttggcaa ggttcatgtt ttataattct aattgtagtt gattttgacg ttgtatcaac
    13321 tatcttttgt agaaacaaat ttattaaaag tgaggaaatt atatatgaat gattttgtga
    13381 ttgaaacaaa gcaacttaca aaaatttatg ggaatcaaac agtagttgac aaagtcaatc
    13441 tccatgcaaa aaaaggccga atatatggtc tgttaggacg caacggagct gggaaaacca
    13501 caattatgaa aatgatttta ggattgactt caatcacgta tggcgaggta gatgtatttg
    13561 gagagaatat taagggacgt gaaaaacaag tatatccccg tatcggtgca attatcgaaa
    13621 cgccaggatt ttatcctaat ctgacaggaa cggagaactt ggagattttt gcaaaactgc
    13681 gtggtacagc cgcccctaat tctgtaaaaa gagcattgga aattgtaggc ttaccttata
    13741 aaaataaaaa gctgtttagt gaatattcac ttggcatgaa acagcgactt ggtattgcca
    13801 acgctatcct gcatgaccca gaactgttag ttttagatga gccaaccaat ggtcttgacc
    13861 ctattggtat tgcggagatg agaaagttta ttagggactt gagcgttgag catggaaaga
    13921 caattcttat ttccagtcat attctttcag aaatctcatt gttggctgat gatattggaa
    13981 ttatcgacca cggtgttctt ttggaagaaa gtagtatgag tgaactagaa aagaaaaaca
    14041 gtaaatatat cctgctacaa gtttcagata ttccgaagac atcacttatt ttagagcgtc
    14101 aatttaacac gaaggattat tccgttcaag atgactacac gttacacctt tataacacta
    14161 atctggatat gggtgaaatc aataaagcac ttgtaatgca gaatgtaact gttattagtt
    14221 ctcaactttg taatgatact ttagaggact actttaaaaa aatcacagga ggagagggca
    14281 ttgcttaaac ttattcaaac agaatttctt aaactgcgtc gtaggaaact tatttggctg
    14341 atgttgttgt ctgctctcat tatgccgttt ttcgcactac tatacttcaa ttattttggt
    14401 aaaacaggtg ttgagccaat aaagttttat aaatggtctg catttggtta tacactttgg
    14461 attattttgc ctgttgtttt gggtatattt tgcaccatgc ttatacacga agaaaaccag
    14521 tatgatatgc tcaaacagct ttggattgta cctattagta aaatgggata cttttttagt
    14581 aaatttttcg ttgttttgat ttactctatt tgttttatgc tgattacagc cgttgcttct
    14641 gtgttattta gtgtactttc tggatatgtc gtatttagct gggcaagcat tttatatttg
    14701 ttgaaaaaat gtttagaaat tggtgtaatc actgcttttg taatgttgcc gattttggca
    14761 atcgctacat ctcaaaaagg atacattctc ccaatttgta tcacattggt atatgccttt
    14821 tccggttttg ttataatgac aataaatatg tatttacacc cattatccag tatgggagtt
    14881 attatcatga gaaataaaga cattccaggt cttatatttt cacaggaaat taatgtccca
    14941 ttagcatttc tttgtatttg tatttggagt attgtatcag tattgtttgc aaacactgca
    15001 ctaggaagaa gaaagtgagg tgtagataat gtggaaatta ctttgggctg aatgtcaaaa
    15061 aattcgtcgt tcaaaaattg tttggattac agtttttgca tccattatga tagcagtcat
    15121 agtttttgca ggagggcagg atgtatataa tgacccagat ttacattatg gcttaaagag
    15181 cgtttatgac ggttcacggt atatagacaa tgcaggttgg tatatggacg cagtacaacc
    15241 ttggtcaact ttttttgttc ttcctgctgt cattgcccta ttaggaagct atattatttg
    15301 tcgtgaagaa gaggaggaca ctattaaatc cctgcgtctt attcccataa atgaaacaaa
    15361 attaaccata gcaaaaatga ttattacttt tatattcagt atacttcttt atttgttact
    15421 ctttactata acatttttga ctgaatccgt tttacatttt tccgatttat cgacaaaact
    15481 ggttttaagt tgcatgaaag agtattttct tgatggcata ggtgtattct ttgcaatttc
    15541 tcccattatt gccttggtat cgcgcatgaa aaaaggatat tggcttgcat tagtattcac
    15601 agaagtttat tctattgctg gactatttgc gggtatgtca aatgtcttac aaaccttttt
    15661 tcctattgcg gcaatattta atatttctgg atactatata actactggag aaaaaattat
    15721 ggggagtatc ataagtttat tactttgtgc ctgcctttct gcatttatac ttaaagggct
    15781 aaaacacaat gaaaaaaatt gaaataggta tatcaaaatg aaaaagataa aaaaactgat
    15841 tattctaatt gtattcttgt ttctttttgg catgttacac tgttatactg aatggaaatt
    15901 caacaataac ccacctaaag gggtaataga aagaataaat gaatggactg ataagattgg
    15961 actggattag taacttatgt taattatagt gaatgtaata ttagcaagtt attgtttcgt
    16021 attttaatac tataacttta aatatttggt tttaatcttc caaacgaata taaaaaaact
    16081 gtcacagagg caacatattt ttgaatattt ataaagtggt agttttgatt ttcaaagctg
    16141 ccgccttttc ttctatataa gcaaacttat cctcaacaaa gtgtaaaaca tggtacattt
    16201 taagaaaaaa gtaccacaga cacaagcaat aaaagaaacg ccgagaacct acaaaaacgt
    16261 agtgcttatg cagatttacg acgccttcta ttttctcaac cgtcttaatg accttgtaca
    16321 aaagaaattt ttctaatatt ttttataatc ttatcttgtg atgtttttta tgtataaatg
    16381 acttaataac aaatccaata aaaattatgg atattggtat aattagaacg aggaatttat
    16441 aatcattata atgtgacatt attcttaccc aactctcaga aaaaatatat cctatactga
    16501 ttaatgaagt attccatatg gttattccca cgatagatga tattacaaaa gttataatat
    16561 tttgacctgc tatacctgct ataaaagata tgtatgttct acataaaggg attaatctac
    16621 atacacaaac agaaatagaa gagtatttgt taaaataatc ttgagatgca aatattcctt
    16681 tctttgcctt tggacatatc tctattattt tatttatgat agatttacct ccccaatttc
    16741 ccactgtata acaaaatatc gaaccaagta tacctgcaat tatacttaaa attaaaattg
    16801 tcagaaatcc aaaatgattt cttgcagcaa ttgctccaca taaaggtaaa acaacttcgc
    16861 taggtaatgg aaaacaagca tactcaatca taattaatac aaatacagag attaatccat
    16921 attgtaaaat aaaactattg attacagaca cttacttatc tatctccttt cttatttaaa
    16981 ggttttaagg gtacatgttc taaggattct caccctatat ttaaagaatc aatctcttta
    17041 aactattata tgtatttaca caaagcattt ataacatact ttaaaaatag taccttaaaa
    17101 taagaaactt aataaataaa aaaagattac gtggctacgt cctactctcc caggcagttt
    17161 cccac
//
LOCUS       DAYASV010000320        11816 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.guided.117, whole genome shotgun sequence.
ACCESSION   DAYASV010000320 DAYASV010000000
VERSION     DAYASV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.guided.117"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            81..1487
                     /locus_tag="ACGI68_003881"
     CDS             81..1487
                     /locus_tag="ACGI68_003881"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFH0140239.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLEFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1515..2363
                     /locus_tag="ACGI68_003882"
     CDS             1515..2363
                     /locus_tag="ACGI68_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0140240.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3024..3716
                     /locus_tag="ACGI68_003883"
     CDS             3024..3716
                     /locus_tag="ACGI68_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0140241.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3713..4648
                     /locus_tag="ACGI68_003884"
     CDS             3713..4648
                     /locus_tag="ACGI68_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFH0140242.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            4796..5719
                     /locus_tag="ACGI68_003885"
     CDS             4796..5719
                     /locus_tag="ACGI68_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140243.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5703..6473
                     /locus_tag="ACGI68_003886"
     CDS             5703..6473
                     /locus_tag="ACGI68_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140244.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6483..7265
                     /locus_tag="ACGI68_003887"
     CDS             6483..7265
                     /locus_tag="ACGI68_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0140245.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7642..8178
                     /gene="rbr"
                     /locus_tag="ACGI68_003888"
     CDS             7642..8178
                     /gene="rbr"
                     /locus_tag="ACGI68_003888"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HFH0140246.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8210..8599
                     /locus_tag="ACGI68_003889"
     CDS             8210..8599
                     /locus_tag="ACGI68_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFH0140247.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8636..9022
                     /locus_tag="ACGI68_003890"
     CDS             8636..9022
                     /locus_tag="ACGI68_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HFH0140248.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9149..10591
                     /locus_tag="ACGI68_003891"
     CDS             9149..10591
                     /locus_tag="ACGI68_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HFH0140249.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10888..11703
                     /locus_tag="ACGI68_003892"
     CDS             10888..11703
                     /locus_tag="ACGI68_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0140250.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEMRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4235 a         1478 c         2252 g         3851 t
ORIGIN      
        1 aaagaaaaga gttaatatag agtgaactta ttgcaatgaa tcaacaaaga aaataatcaa
       61 tataaaaagg agaaatattt atgaatacag gatttccaaa agacttttta tggggagcat
      121 catcttctgc atttcaagtg gaaggagctt gggataaaga caataaagga aaaaccgtag
      181 ccgactataa ttcttttaaa aagtctcatt tacaggcaga tactaaagtc gcatcagact
      241 tttaccataa ttatgaagaa gatattgaat taatgaaaga attagggatg aaaacatatc
      301 gtttttcaat ttcatgggca agaataatac cagatggtga aggagagata aaccagaaag
      361 gtttagaatt ttataataag ataatagaca aactgataga gtgtgatatt gagccattcg
      421 taactttata ccattttgac cttccattta aattagtaga gaaatataat ggttgggaaa
      481 gtagagaaac tgtctatgct tttgaaagat ttgctaaaat atgctttaag cattttggag
      541 atagggtaaa gtattggcaa cctcataatg aacagaattt aattgttaga gtggaggaga
      601 gaattaatat atatgatgaa acagattcat ggaagataga taaaatgcgt gctcagatgg
      661 attataatct ttgcttagca catgcattag cagtcaatgc atgtcatgag atgataaaag
      721 aaagtaaaat tggagcagca gtatcatcat ctgtaacata tcctcttact tctaagccag
      781 aagatgtata tgcagctaga atgaatgata attttaaagt gtactatatg cttgatatgc
      841 atcattatgg agaatatcca gggtattata tgaagtatct tgaaaaaaga aatatagttc
      901 ctcacatgga agatggggat aaagagatac ttaaaaaagc taaaatggat tttatagctg
      961 taaattatta taggacaaat tgtgcagaag ctttacctga agattcacaa catccatttg
     1021 gattaagaga aggaactgtt gattttagta tgtatggact atttaaaatg tcaatgaatc
     1081 ctaacttaga ggcttctgag tatggagcag caatagaccc atcaggtctt agagttgcct
     1141 taaatgaata ttggcaaaga tatcacttac ctgttataat aacagaaaat ggtctaggag
     1201 ctaaagatat attagaagat ggaaaaattc atgatgacta tcgtatagac tatttaagaa
     1261 gtcatataaa tgcatgtaaa ctagctattg aagatggtgt agagatgatt ggatattgtc
     1321 catggtcatt tacagacctt ttaagttctt ctcaaggttt taataagcgt tatggacttg
     1381 tttatataaa tagaactgac catgaagttt tagatttaaa aagaatcaag aaagatagtt
     1441 tttactggta taaagaagtt atagagaaca atggaatagt aaaataaaca taaatatata
     1501 ttgagggttt tagtatgtac attacaaagg tattaaataa ttcgttgcta cttgcaaagg
     1561 atgataatga tgaagaaata atattgatgg ggaaaggcat tggccataat tataaggccg
     1621 ggtctgagtt aaataaaaaa gatatagaaa agatttttgt tcttcatgat gaaaatataa
     1681 aaaagagtat tatacaattg gcaacagaaa tagatgcaga atattttggt atagctcaaa
     1741 tgataattgc atatggaata gaaaaatata acttaaaact tatgaaccat atatatttgg
     1801 ccttaactga ccacattgcc tttgcagtga gacgttttaa agaaggtata atgattgaaa
     1861 atcactattt gttcgaaatc aaggaattta atccaaaaga gtatgatata ggaaaatatg
     1921 ctattactgt ttttaaggag gttcttggtt tagacctacc agaagaagag attggtaata
     1981 tagcaggcca ttttataaat gcacaacaag ataatcctta tagtgataga aataagagaa
     2041 gtgctaaaat tgtaaatgct attttgcaaa tagttcatta tcacttttct attgtatata
     2101 ataaagagtc attttattat aggagatttg taatgcatct aaaggccttt tcacaaagat
     2161 ttctggctaa tgaaccatca agagaaaaaa tagattttat atatgagcaa gttcaaaaaa
     2221 attgtaagga agagtatgaa tgtgtgaaaa aggtaggagc ttatatgtat aaggaatact
     2281 ccagagaatt accaagacaa gaggagttat atttgatgat tcatattcat aagattttag
     2341 gagaattatg cgaaaatagt tagaatatct taatatgcta gtaatattat aaaaaattaa
     2401 aaattaactg cactttaaat atgaataatt gcattactta agggggttgt cttaaaattg
     2461 atttttttaa ttaaatttga agctcaactc cttttttaaa cctttatatc attacaactt
     2521 taatgtttat acgactgtac tttagatgta tatctacttt ttacatttct caacatgcaa
     2581 acctaccatt ttttgcagta tgttaattga aataaaccat tgagttaacc ctaaatttat
     2641 tctggactta taggaatagt tccaaagata ctttaaaata ttttctggaa taattgctta
     2701 tttcacatat gttttattgt gatgttttga tttaataaat tcttcaactc tgtatatagt
     2761 aaatgtatta tcatgtttgt tttattcaag cacacttata atacgcttga ttttttcaca
     2821 atgatgaaat atactaatgg gtgttatagg tataagaatc gcattacact tgaagaagta
     2881 agtgataaat gaggtataaa tcctcatatg ataaactact atttcattga ggaacatatt
     2941 ctaagtgatg tcaagatact acaatatgac taaattccta gtgataaaaa agtctacgaa
     3001 tgagtgtaga aaggggaact aagatgaata aagttttaat tatagatgat gataaagatc
     3061 tttgtatttt gttaaaaaag agtatttcaa ttgaacagat tgaagcaaac tattgccatt
     3121 ctgggagaga tggactggtt gaactttcaa aacaagatta tcagttagta gttctagatg
     3181 tgatgatgcc aggtatggat ggttttgaaa ctatggaaca aatacgaaaa tatagcagta
     3241 ttcccatctt aatgctgaca tcaaaaagcg ataattacag caaagtgcat ggattgcgag
     3301 ctggtgcaga tgactatcta accaaaccat ttgaaattac agaatttact gctcgtgtac
     3361 tgtctttaat ccgacgttac acccgtttta atccaaaaaa tgaggagcca cagcaacttt
     3421 ctttcaaagg tttgttgatt gaccttgacc agcatacagt tactaccaat ggcgaaccta
     3481 tgttgcttcc acccaaggaa tttggtgtac ttctttactg tgcaaggcat cagggtaaga
     3541 ttttgacaaa acagcagatt tatgaagccg tgtggggtga gccatatgta tatgatgaca
     3601 gcaatatcat ggctattatc agtcgcctaa gaaaaaaaat tgaactagac ccatcatctc
     3661 ccatctattt gcaaacaata aaaggaattg ggtatcaatt caacacggag gtatgaaaat
     3721 ggatgaacaa acgataattc ttttcttact cttgctgtcg attggtctac tttttctttc
     3781 catatttgct ttttccaagc ttgggcatat ttacaagcgc ctaaaagata tagaggagat
     3841 actggctgat gtacaaattg gagacgaaaa tcgtaaaata ctaattaagc catgtgatgt
     3901 tatggctcca ttggtttatc agcttaatga gattgtctac gattatgaaa ataagttact
     3961 ttctctgaaa aagtcggata aagctagcaa acaacttatg acaagtttat ctcatgatgt
     4021 tcgtacacca ttaacaactc tgattggtta tctggatgct gtacacagtg gtatagttat
     4081 cgaacaagag cgggaggaat accttgaaat cgctaggcgt agagcctacg acctcaagga
     4141 ttacattgat gttctttttg actggtttcg gttgaactct gatgaattta cgctgtcaat
     4201 agaatctgtg gaaattgcag agttgtcacg caatattttg aaagactgga ttcctatatt
     4261 ccatgaaaag aaactgaatt ttgaaattga tattccagaa aatcgactaa tggtaaacct
     4321 cgacccagat ggatattccc gtgtggtcaa taatctggtt caaaatgttc ttgctcacag
     4381 taaggctcgg caaatcaaga ttaccatgag tgaggattcc agaatggtat tactccgtgt
     4441 tgaggatgat ggtgttggga ttgccagaga aaatttacca catatttttg agcgactgta
     4501 taagtgtgat aaaggacgtt cagaaaaagg aagtggtctt ggtctatcta ttgtcagtca
     4561 aatggtggag cgaatgggtg gtcaggtttc cgtagaaagt gagattggaa agtataccgt
     4621 atttacagtg agcttaccat taatataaaa ttgtttttcc cccatgtgac atgtatacac
     4681 atggggattt tttgtaagtt ataaagttgc aaggttaata caaggttgtt gcaaggttaa
     4741 tgtaagattg aggtggtatg ataactttaa taaacaaagg aggcttttat tatttatgaa
     4801 taatttaatt attgaaacaa aaaatctcac taagcaatat ggtgctcaaa agagcgtttc
     4861 taatttgaat attcacgttc agaaaggtcg catttacgga ttgctaggaa gaaacggtgc
     4921 gggtaagacg acaactatga aaatgttatt gggtctaaca aaaccaacta ctggagaaat
     4981 taaaatattt ggtcaggaca ttcacaataa cgagaagaag cttcttcctc gaattggatg
     5041 tttgattgaa tcaccagggt tttatcctaa tctgacagga acagaaaatc tcaaaatctt
     5101 tgcaaggctt agaggaatac caaaaaaaaa tgcagtagaa tcagcactgg atgtagtcgg
     5161 tttgccaaac aatgacaaaa agttattttc tcaatactct cttggtatga aacagaggtt
     5221 ggcgattgct cttgccatta tgcatgaccc acaactgttg attttggacg agcctatcaa
     5281 tggacttgat cctattggta ttgcagaagt aagaacgttt attcgtgatc tctgcacaga
     5341 acgaggtaaa actattttga tttccagtca cattctttct gaaattgcct tgttggcaga
     5401 tgacattggc attattgacc atggtatttt gttggaggaa gaaagtcttg cccaattaga
     5461 agaaaaaaac agtaaataca tacattttgc tgtatctgat gcagcacagg cagcaaggct
     5521 tctggaaacc caacttaacc tgaaaaattt caaagtagca gataattaca atattatgct
     5581 atatgataca gatgtttctg ttgcagaaat aaatcgtgct ttcattctaa acaacattga
     5641 ggtatcagaa tctcatttat gcgaagatac tcttgaagat tacttcaaga aagtaacagg
     5701 gggtgaagga attgcttgat ataatttcat gtgaattttc aaaactcaag cggttaaaat
     5761 ttatactgat ttctatttta ggtgcttgct tatttccaat tcccgcaaca attttgattg
     5821 caaaggacaa cttgcccttt gagcaattgt tcaaattagt tgtcaatttt ggatattttt
     5881 tgctattacc aattgtatta agtattgttg ctagccaact attttttata gaaagagata
     5941 atgatgtatt aaaaaatctt gcaacagtgc cagtgcaaaa aggaaaactg gctctggcaa
     6001 aattggctgt gttgctgttt atatcattgt tctactctgt tgcagggtta ggagctacta
     6061 tcattggagg tttcattgtt ggaatagtag agggggttgc tgtaaaatta ggaatgagca
     6121 ttgcttttgg aattatgttt tttgttactg tacttcctgt tgtggtactc attgtatatt
     6181 tcaatcgcag ctatattttt tctattatac tttcttttgt atatgctatt ttcaatttta
     6241 gtatatcctt aaatattatc aattttgaac caaataaccc aatattaagt gtattacctg
     6301 ctcctgttat aatgcgttgg tggatggcat tttggggaga tcccactggc gaatacacgg
     6361 cacttagaca gccatatttg ctctcaactc ctgcttgtgt aggtatttta tgtttgataa
     6421 ccattatagc agtcctgctg atttgtatga tatacaaaaa gcaggagaat taggaggtat
     6481 ctatgcttga tataattact acagaatttc aaaaaataaa aagatataat attctatgga
     6541 ttggtgttgt tgctgttctg ttttcagcac tattggcagt ttttcaggtt gtaagttcac
     6601 acggcagtga ccctctaacg tatgagagct ttgcaaacgg agttatctgg aataatttca
     6661 gtcttagttt tccatttggg attacactta ttggaggata tcttattaac cgtgaatata
     6721 cagaccaaac cttaaaaaac atcctaactg ttcctatttc attacgcaaa ttgctagtgg
     6781 gaaaattaat tgcggttgga ggaattactg tattgttaag tcttttcagc ttcttatgca
     6841 catttatttt ggggttgttg ttttgcaata tcaatatatc tgttacactc gttgtaaaat
     6901 ccttagttca gattttgtct gttaatctgt attgttatgt tgctgttttg ccgattattg
     6961 cttattttgg tcgtaaacaa aatgcatttc tgacgggtgt agggattgca tttgtatacg
     7021 gtttttgtgg tgtgtttgtt gctggaagaa atctgacaga cttttatcca atcacagcag
     7081 gtcttggtct agttggatac aacaatggtg ctggtactgt ttatcagcca cttatcggtg
     7141 ctatgacttt gattgtaatt ctaatcttga caactattct acttgtattt acacctaatt
     7201 atgataaagt tatgaccata tctaagaaaa aagggataaa gcataaaaag tattctcata
     7261 aataacataa atattgtttc tattatttaa gttgctagac agtgaaaaga aaaaatctgt
     7321 cgtacagtgt ctatatgctc atgtattcaa gaagctacag ttttgatttt tcataactgt
     7381 agcttctttt tgtatttatt aaatcacgac tatcataata cttgcaatag gtatagcgac
     7441 aagagagagt actgtcatat gtgttttata tattacatta agtatattct aaaatatatg
     7501 ttttaaataa tatatttgaa aataggaata tttaaataaa aaaaataata ttatatatag
     7561 ttgacaaaaa ttggcaaatg agataatata taaatataga aatagtaatc attactatta
     7621 ttaaaaaggg aggaattaat tatgaactta aaaggaacta aaacagaaaa gaatttaatg
     7681 gcaggatttg caggagagtc agaagcaaga aataaatata catactacgc ttcaaaagca
     7741 agaaaagaag gttataatca aatagctgct atatttgaag aaactgctaa caatgaaaag
     7801 gaacatgcta aattatggtt taaactttta catgatggta tgccttcaac agaggaaaat
     7861 ttaaaggatg cagcagcagg tgaaaactat gaatggactg atatgtatgc aaaatttgct
     7921 aaagaagcta gagaagaagg atttgacaag atagcttatt tatttgaagc tgttggaaaa
     7981 atagagaaag agcatgaaga aagatactta aagcttttag aaaacttaaa tgaaggaaaa
     8041 atatttaaaa gagatgaaga agttgtttgg caatgtcaaa actgtggtca tgtgtatgta
     8101 ggtactgaag caccagaaaa atgcccagta tgtgaccatc caaaagcgta tttcaatata
     8161 aaagctgaaa attattaaaa attatcatta ttaaaaagga atgttaacta tgaaattttc
     8221 taaacaacga gaactgattt taaatgaaat attaaataat ccggttcatc ctactgcgga
     8281 ttacctatat gaaaacttaa aaaaagataa tccaaattta agcttaggaa ctgtgtatag
     8341 aaatttagct cagctaacgg aacatggctt tataagaaag gttagtattc cagggtatcc
     8401 agatagattt gatggtcgaa tagataatca ctatcatatc atatgtgaag tatgtggaga
     8461 ggtatacgat ttagaatccg aagttcttaa taatttacaa gaattaatat ctgaggagac
     8521 agacataaaa ataacatctt ataacataag ttttaaaggg atttgtaata attgtaaaag
     8581 gtgtagccaa gtaggttagt tttcaaattc taaaaataat taatgaggtg agtttatgtg
     8641 tagtgaacaa aaatttttta tatgtgagac ttgtggaaac cttgtaggaa tgatacaaag
     8701 tggtggtgta cctattttct gttgtggaaa acctatgaag gaacttgttc ctaatacaac
     8761 agatgcagca gttgaaaaac atgtacctgt aattgaagtg gatggaaata atgttactgt
     8821 taaagtaagt agcacaactc atccaatgac taaagaacat catatagcat gggtgtatct
     8881 tatgactgaa caaggtggtc aacgcaaatg tctagcagta gatggagaac ctgtagttaa
     8941 atttgctttg aatgatgatg ataaagtgat ttctgcctat gcttactgta atttacatgg
     9001 attatggaag gctgaactgt aatatttaaa taaattgagt caaaaaggag gctataaatg
     9061 tattttcata caaatagcct cctataaatt tatataaatt caagttttaa ttttgtttaa
     9121 tgtcaatata atataaggag aaaataatat gtcaatttat aaatgtagtg tttgtggata
     9181 tatttatgat gaatctaaga atgataaaac atgggatgaa ttaagtgaag attgggaatg
     9241 ccctgtatgt acaaaaggtc gtagttattt tggaaagata agtactgttt actatgaaga
     9301 agatgaaaag atagcagaag acatagttga agatgaatct aaattaaata ctgaaaaaga
     9361 gggagattta aattacttaa gtacatattt aagaagagat gatgaagttg agaaacatat
     9421 ggatataatt cacgaaatgg ctgttacagg aaaatctata atagaaccta tgagaactaa
     9481 actaccagta atttcttggg atgatatttt aattatgggt gctcaattaa atccattacc
     9541 tttaaatgag catgatgaag taaatacaac aacaataata ggaaaaaaag caaagaaacc
     9601 tatgattata gaaaatccag tttatatttc ccacatgtca tttggagctt tgtctaaaga
     9661 actaaaaata gctttagcaa agggtgcagc ccaaaataag acagctatgt gtagtggtga
     9721 aggaggtata ttaccagaag agaaggaagc ttcatacaag tatatttttg agtatgtacc
     9781 aaataaatat agtgtaactg aagaaaatct taaaaattct gatgctatag agataaaaat
     9841 tggtcaagga acaaaacctg gtatgggagg tcatcttcct ggcgaaaaag taactgaaga
     9901 aatagcaaaa gtaagaaata agcctgtagg tcaagatgta ataagtccat cttgttttga
     9961 ggaaatacaa tccaaagaag atttaaagaa acttgtagat gaacttagag aagtttcaga
    10021 agggcgtcca ataggtgtta aaatttcagc aggtcatatt gaaaaagata tggaatttat
    10081 agcttatgct aaacctgatt ttgtaactat tgatggtcgt ggtggagcta ctggagcaag
    10141 tccaaagttg cttaaagatg caacttcaat tccaacaata tttgctcttt atcgtgctag
    10201 aaaatacata gatacacatg gattagatat agatttggtt ataacaggtg ggcttcgtat
    10261 atctactgat tttgctaagg caattgctat gggtgctgat gcagttgcta ttgcaagttc
    10321 tgctcttatg gcagcggcgt gtcaacaata tcgtatatgt ggttctggta agtgtcctgt
    10381 aggagtagct actcaggatg aggaattgag aaaaagatta catatagaaa acagtgctaa
    10441 tagagtggca aactttttaa atgtaagttt agaagaactt aagacttttg ctagaatttc
    10501 tggtcataaa gatatacatg atttaagtgt tgatgattta tatactgtta actctgaaat
    10561 atcaaattat acaaacatac agcatgtata gattgaagtt tgaaactatt agattttaga
    10621 tagctataaa agtaaatgat aaatatattt tataaaagag agtgtcttaa aatgaacttt
    10681 ttagttcata agtcactctt ttttttatga agccaaatat atttatcatt ttagcaatga
    10741 aaaagaatct tatctcttta ttttgagata gactccttct atttatgaaa attaaagata
    10801 ttaaattgta aaatattctg tgatgtcgta ctattaaact aaagattctg tactttaaag
    10861 taaataaaaa tttaaggagt gattattatg gaaagattga agatacaagt tttagttgac
    10921 aataatactt atattgatag atactttgtt ggagaaccag cagtaagcta ttatattgag
    10981 attgatggaa ataggatttt gtttgactca gggtattcag atgtattcat atcaaacgct
    11041 gaaaaattaa atatagattt aggaaattta actcatgttg ttttttctca tgggcataat
    11101 gaccatacaa gaggtatcca gtttctagaa aataggtatg atttatctac agttgaactt
    11161 atttcacatc ctaactgttt tatacctaga aaacatggag agaaaagcat aggagctcca
    11221 ttttctgctg aggaaattaa aaatattttt atgtataatc ctaaggataa gccatttaat
    11281 ctaagtaaga attgtgtgtt tttaggtgaa ataccttcta taaatgattt tgaaaaaaga
    11341 gctaaaattg gtaaatgtaa aataggagat ttgtgggaag atgattatgt tttagatgat
    11401 tctgctatag tttgtaaaac tgataaaggt atttttattg ttacaggatg ttctcatagt
    11461 ggaatttgta atatagtaga gtatgctaaa aaggtatgtg gagatgatag agttattggt
    11521 atactaggtg gttttcatct ttttgaattg aataataggt tagatagtac aatacaatat
    11581 ttggataaag aagaaatgag aatgttgtac ccttgtcact gtgtttcttt taaggtaaaa
    11641 gcgaagatga atgaaatatt gaatataaat gaagttggag tagggctgac cataagtata
    11701 tagtatttga aagtatataa gatttaagtt gaaggttaaa aagatatttt ctatagaaaa
    11761 tatctttttt gttacgttta ttttaagaga aatcaatata cttttatatg aaatat
//
LOCUS       DAYASV010000321        11063 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.guided.124, whole genome shotgun sequence.
ACCESSION   DAYASV010000321 DAYASV010000000
VERSION     DAYASV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.guided.124"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            246..863
                     /locus_tag="ACGI68_003893"
     CDS             246..863
                     /locus_tag="ACGI68_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFH0140251.1"
                     /translation="MCKKTYLLVVSMLIAGLLTACSGSPTKDSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDLNDIMTPQQAMEIALKEQNG
                     IVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            971..3502
                     /locus_tag="ACGI68_003894"
     CDS             971..3502
                     /locus_tag="ACGI68_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0140252.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLYSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3936..4637
                     /locus_tag="ACGI68_003895"
     CDS             3936..4637
                     /locus_tag="ACGI68_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-G-Cd"
                     /protein_id="HFH0140253.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4687..5769
                     /gene="vanS"
                     /locus_tag="ACGI68_003896"
     CDS             4687..5769
                     /gene="vanS"
                     /locus_tag="ACGI68_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS"
                     /protein_id="HFH0140254.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIIIFKVIIPIVGNL"
     gene            6030..7130
                     /gene="vanG"
                     /locus_tag="ACGI68_003897"
     CDS             6030..7130
                     /gene="vanG"
                     /locus_tag="ACGI68_003897"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HFH0140255.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNDDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7127..7933
                     /locus_tag="ACGI68_003898"
     CDS             7127..7933
                     /locus_tag="ACGI68_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFH0140256.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7951..10089
                     /gene="vanT"
                     /locus_tag="ACGI68_003899"
     CDS             7951..10089
                     /gene="vanT"
                     /locus_tag="ACGI68_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HFH0140257.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKRLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSSPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSASEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10403..11023
                     /locus_tag="ACGI68_003900"
     CDS             10403..11023
                     /locus_tag="ACGI68_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFH0140258.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
BASE COUNT         4193 a         1158 c         1986 g         3726 t
ORIGIN      
        1 caaaatgaac tttttagttc atgaggcact ctttttgtac gaaataaaat atatttttat
       61 tatttcaaca atgaaaaaga ggcttgtttc cattttgaga cagtataatt tttatgtaac
      121 tttaatttta gtaaatataa agttttaaaa ttgataaaat aggaggaatt tttaatataa
      181 aagggtaaat aaagggttta gagggcataa tatagaataa attaatatat ttaaggaggg
      241 tttttatgtg taaaaaaacg tatttattag tggtaagtat gcttatagcg ggtttattaa
      301 ctgcatgtag tgggagtcca actaaggatt caggacaagc acaagataat acaaaaaagg
      361 aggcaaatgc atcaaataac gctttaaaag atgaaaaaaa taatgaaaat ttaatggaac
      421 aagattttaa agtgccatac acagatgcaa taaacatatt taaagataaa tataaggatg
      481 ctgatatagt agatttaagt cttgagagag atttaaataa atttgtttat actgtggaag
      541 gtgtagatga taataatgaa tataagatga aaattgatgc taatacaaaa gatgtattag
      601 aagataaaac agaaaagttg gattcagaag atttaaatgg agttgctaga aaagaaaaat
      661 tagatttaaa tgacataatg acacctcaac aagctatgga aattgcatta aaagaacaaa
      721 atggaatagt taaagaatgg agtttagaca aagatttaga tgttactttt tataaaataa
      781 gaatagataa agataaaaat gaatatgaca taaaagtaga ttctaaaaag ggaactgtat
      841 tgaaggtaga aaaagaagat tagaagattt aaactatctt taaaataatt tcttttttga
      901 ataaattttg tttaaaatat aacaagcagg gtataaagag actaaataac tattttagga
      961 ggagtacggt atgtcattga aatcactaaa aataaaagaa aatttatatt gggtaggttc
     1021 gcttgaccct gatttaagag tatttgacat aattatgtat acaccttatg gaactactta
     1081 taattcatat gtattaaaag gaacagaaaa aacagtttta tttgagacag ttaaagataa
     1141 gcattttgac aattatattg aaagattaaa tgatttaaat atagattttg aaaaaataga
     1201 ctatattgtt gtaagtcata ctgaaccaga ccatgcagga agcgttgaaa agcttttgga
     1261 tttagctaaa aatgcaaaag tagtagcttc agaaacagca attaaatatc ttaaggaaat
     1321 cgtaaacaaa gactttgaat atgttgcagt tacagatgga gatacattat caattgggga
     1381 caaaacttta gaatttttct ctgtacctat gcttcattgg ccagatacca tatacacata
     1441 tataaaagag gataaaactc ttgtaacatg tgattcattt ggtagtcact atagtaatga
     1501 taagatagta aatacacttg atgaaaatga agaaaaggat tacttggatg ctttaagata
     1561 ctattatgat tgtataatgg gtccatttaa accatctatg gtaactgcta tagaaaagat
     1621 aaaagattta gatatagata ctgtatgtcc aggtcatggt ccagtattaa ctgaaaatcc
     1681 tagaaaaatt atagaccttt attacaactg gagtgtaaat gaacaaataa agttagaaaa
     1741 agaagttaca atttgttatg tttctgcaca tggatataca aagattatgg cagaagctat
     1801 aaaagcatat attgaaaaaa atagcaacta taaagtaaat ttatttgatg taatagagca
     1861 taagcaagaa gatattttgg caaaaatagc agtatcacaa ggtgtattat ttggaacacc
     1921 aacaatatta ggagatgcgt taaagcctat atgggatata ttaatatcgt taaacccagt
     1981 tcttcatggt ggaaaagttg cttcagtatt tggttcatat ggatggagtg gagaaggtat
     2041 agaaaatgct atggagagaa taagtcagct tagaatgaca gcagtaaaac cttttgctgt
     2101 taactttaaa ccatcaaatg aagagattga taaattatat tcttatacag gtaaattcct
     2161 agataagtta aattctactt ttggaagtaa aaagaaaact aagaaattta aatgtgtaat
     2221 ttgtaatgaa gtttttgaag gtgatagtgc tcctagtgta tgccctgtat gtggagctaa
     2281 ggaagaccaa tttatagaag ttgaagaaga tgaagttact tttagaaaag atactgatga
     2341 atatttcgta atagtaggaa atggagcagc tggtttttat gcagcagatg ctataagaaa
     2401 aagaaataaa acttgtaaga taactatgat ttctaatgaa gatgagttga cttactatag
     2461 acctgcactt tctgatggta taaatgaaga acttggttca gatttctaca tggaagataa
     2521 agattggtat gataaaaata atatagtagt aatattagga acgaatgtag ataaattaga
     2581 tgaagtgaat aaaactatta tagtgaatga tggagctatt aaatttgata agcttgtaat
     2641 agctacagga agtagaaact ttataccacc aattaagggc catgatttag aaaatgtgtt
     2701 tactcttaga aatataaaag atttatatag tgtaaaagaa gcactagaaa aatctaagaa
     2761 agttgtagtg attggtggcg gtttattggg acttgaagct gcatgggaat ttagactaaa
     2821 aggattagaa gtagtagtag ttgaagccat ggatagcata ctatcaaaac aacttgataa
     2881 agaaggtagt aaaatattag aacaatgtgt aagagataca ggaatagatg taagattggg
     2941 tgtagcagtt gatggtattg aaggtgatgg aaaagctcaa aaagttgtat ttaaagatgg
     3001 agatagtgtc gactgtgata tggtagtatt ttcaattggt gtaagagcaa acactcaaat
     3061 ggttcaggat acatctgtaa aaattgatag aggtattgtt gtagataaga cattacagac
     3121 aaatgttaaa gatatatatg cttgtggaga tgttgcacaa gtgggaaata ttagtttagc
     3181 aatatggcca tcttcagtag aaatgggaaa aatagcaggg gcaaatgcaa gtggagataa
     3241 tttgacattt gaaagtgaag tatatccagt ttcattagat gcaatgaatg taaaagtatt
     3301 tagtattggt aatatacaaa attttgataa agaaatttca tcaaaagatg aaggtcaaag
     3361 aatatataaa aaattattta tgaaagatgg ttcattagtt ggagctatac ttataaatga
     3421 tttaagctgt actgttaaat tgataagatt aatatctgaa aaaggtgatt ttgaggatat
     3481 tatgaaggct gatatactat aatttaaata cattaaaata aaaaggagtt gtctcaaaat
     3541 tgattttttt ctaatcaaat tttaaattca actccttttt tgaattttta tataaaataa
     3601 catggaaatt ttagtaatgt ctgtggttac atacataggt ttaattgttt tttatatgga
     3661 ttgtctctgg tataataaat tgaattgtgt ttgactgata gatattttta ttgaaagtta
     3721 atacctgtta tgcgataata aggacgtgtt aggggagttt ttataataac aattaatctc
     3781 agtttttgtg ttccaatagt agcatcaaca cattctgatg tagtagttct tttcttataa
     3841 aaattagtta caaatatgct tgtaattatt tgatatgtaa atttaccaat attataaatg
     3901 atgacctcga taataaggag agtgaaaaat aatctatgaa cgaaaatatt ttgattgtgg
     3961 atgatgaaaa agaaatagca gatttaattg aagtatattt aaaaaatgaa ggttttacag
     4021 tatacaagtt ttatacaggt aaggaagctc tagaatgtat tgaatctaaa aatttggata
     4081 tggctatttt agatattatg cttcctgata ttgatggatt tcatatttgc cagaaaattc
     4141 gagaaaaata ctattatcct atcattatgt taacagctaa aattgaagat atagacaaga
     4201 taatgggttt aaccataggt gctgatgatt atataacaaa gccatttaac cctttggaag
     4261 ttgttgcaag agtaaagaca caattaagaa gatatatgcg atataataat tcttatgaac
     4321 aacagtcgat aattgttaat gagtatgata ttaaaggatt gattatcaat aaagaaacac
     4381 ataaatgtag tttatttggg aaagaagtag cattaacacc gattgaattt tctattcttt
     4441 ggtatttatg tgaacatcaa ggaaaagtgg ttccttctga agaattgttt gaagctgtat
     4501 ggggagaaaa atatcttgat aataataaca cagttatggc tcatataggg cgattacgtg
     4561 aaaaaattca tgaaccttct aaaaacccga aatttataaa aactgtatgg ggagtaggtt
     4621 atacaattga aaagtaaaaa tattaaagtc aatcctgact ttactcaagt gagaaataaa
     4681 atttttatga gaacagcttt aatggcattt atagccatta ttttcatatg gattttatat
     4741 tcttttatat tgtatggtaa gttttctatc tggatagtat cattctttga aaatgctttt
     4801 cgtatggaat ataaagctgc attgaattta tatcagcaga ttttccgtaa tagcatggag
     4861 ctcttcatga tactagctat ggtgattgtg ttttttataa tattccgggt ttatttaaat
     4921 tggtttacaa aatattttag tgaaataaat caaggtattg actctcttat caaagaagat
     4981 gttggagaag tggctttatc tccagagtta ttggcaatag aaaagaaaat aaactcaatt
     5041 aaacatatac ttgaacaacg taaatttgag acacaaatgg cagaacaacg taagaatgaa
     5101 cttattgttt atcttgcaca tgatttaaaa acaccactta catcagttat agggtatctg
     5161 acactacttc gagatgaaag tcagatttct gaagaactgc gaaaaaaata tttatctatt
     5221 tcgttggata aggcagagcg tcttgaagat ttaattaatg aattctttga aattacaagg
     5281 tttaatttat caaatatatc acttgaatac agtacagtaa gcttaactcg tatgcttgaa
     5341 caactaacat atgagtttaa acctactttg atgagtaaga atttaaaata tacactgaat
     5401 gttacaccaa atatgatgat aaggtgtgat gttaataaaa tgcaacgtgt atttgataac
     5461 ttattacgaa atgctgttta ttatagtttt gaagatacta ctattgaaat aacagctgta
     5521 caggaggatg attatataaa gcttaaattt ataaatcata gcaatacgat tccagaggaa
     5581 aaacttgagc gtatttttga acaattttac aggttggata catctcgtaa ttcaaacagt
     5641 ggtagagctg ggttgggact tgctatagct aaagaaatag tggagcttca taacgggaca
     5701 ataacagctc atagcgagaa caatataatt atctttaagg ttattattcc gattgtagga
     5761 aatttgtaag aatttaagta taaaaaaata ggaaattctt taggataaat aaataaagag
     5821 cactcttgtt attgatataa ttactgtatc aaaacaagag tgcttttatt ttgatacagt
     5881 aattatatca atagaaaaat gtgaaaatat attatgtgga acagatgcaa ttgtaagtga
     5941 aggttgattg gatttgaaat agaaaaagta ttttaacatg tttctgtaga ttgtataaaa
     6001 ataaataaat caacaaagga gaatcaaaga tgaataataa aaaaattgca gttatatttg
     6061 gtggaaactc aacagaatat gatgtatcat tacaatcagc ttttgcagta cttgagaatt
     6121 tgaatacgga aaaatatgat attataccaa ttggaattac aagggagggt aattggtatc
     6181 attatattgg agattataat aatatccaga ataacacttg gtttgaaagg gatactgata
     6241 taattccagt tattgtttcg cagaaccgtg tcaaaaatgg tattattgaa tttttaattc
     6301 ctgatgctgt ttctcagaat catattccaa atagtgctat tgaatcatgt gaaaatgata
     6361 ttaaatacac taaacttgat tttgtgtttc ctgtactgca tggaaaaaat ggagaagatg
     6421 gaacagtgca ggggttattt gaattggcag gtatacaggt tataggatgt ggtactcttt
     6481 cttcagcatt atgtatggac aaagacaaat cacataaact tgttcattca gaaggtatta
     6541 aagtaccaaa atcaatatta ctaaaatact cagataaatt taatgtggct gaactgtcaa
     6601 aagacttaac ttatccatta tttataaaac ctgtgcgtgc aggttcatca tttggtatta
     6661 gtaaaattta taaaaaagaa gagttacaag tagcagtaga cttggctttt gaacatgata
     6721 atgaagtaat aattgaggaa aatattgatg gttttgaggt agggtgtgca gttttaggaa
     6781 acgatgactt acttgttgga agagtagatg aaatagaatt gtcaaaagga ttttttgatt
     6841 atacagaaaa atatacattg aaaagttcaa aaattcatat gccagctaga attgatttgg
     6901 attgtgagaa aagaatacaa gagacagcaa aaattattta tagaactctt ggatgttctg
     6961 gatttgctag agtagatatg tttttaactc catctggtga aattattttt aatgaagtca
     7021 atacaattcc tggctttact tctcatagtc gttatccaaa tatgatgaaa ggtattggat
     7081 tgtcttttga agaaatattg gacaagataa ttgaggtgta cataaaatga tgaataatat
     7141 tttattagat aaaaataaag aagataaaat gacaaatact atcttattga ctaaagataa
     7201 agtttatgat gggaatctaa ttctagtaaa tgctttttta ccagttaaaa catctgaaga
     7261 tatagattta attcctgtag atacaaggtt tccaagtatt cttatgaaac gtgaagctac
     7321 aaacatttta caaaatattt taaaaagtat atgtggaata aatgaaatag tacctgtaag
     7381 tggatatcgt actgcagagg aacaacaaga tatatattca agttctctgc gtgacaatgg
     7441 aaaagacttt actaaaaagt ttgtagcact tccaaatcac agtgaacatc agacagggct
     7501 tgctattgat ttagggttaa agaaagaagt tatagatttt atctgccctg aatttcctta
     7561 taatggtatt tgtaatgagt ttcgtaaggc agcactacat tatggtttta tagagcgtta
     7621 tcagtgtaaa aaagaagaaa taacaggaat atctcaagaa ccatggcatt ttcgttatgt
     7681 gggatatccg cattctgaga ttatcaataa aatgaatctc gctcttgaag aatatataga
     7741 atatattaag agattttcgt atacaaattg tttaaaaata gaagataaag aaaaaattat
     7801 agaaatcttt tatgtgccta ttcatactaa agaagaagtg agaatctcta tacctgaaca
     7861 atgtgtatat caaatatcgg gtaacaataa ggatggtttt attgtgacat tttggagaaa
     7921 tagaaaggac taagaaatgg aggaacaaga atgattagga ataaatctta tgcagggctt
     7981 gattatttta ggattatagc tgcattactt ataattgcaa ttcatacatc tccattatta
     8041 tcatatagta aaatggctga ctttgtgtta actagtataa ttgctcgtat tgctgtgcca
     8101 ttctttttta tgctatcagg cttttttcta atttcaacat ataattataa ttgtaataaa
     8161 ctgaaatctt ttctaaaaaa tgtattattt atttacggga tagctattgt aatttatatt
     8221 cccttaaata tttacactgg atatttttcc atgaataatt tagtgcccaa tattattaag
     8281 gacattgtat ttgatgggac tttatatcat ttatggtatc tacctgcatc aattattggt
     8341 gcaagtattg catactttct tgtaaaaaga ctaggtttaa agtactcact agtaatcact
     8401 attgttattt atgtaattgg tatgtttggt gatagttatt atggtttttt agaaaaaata
     8461 ccactattaa agaatttata tgggtatatt tttgaagtat cagattatac acgaaatggt
     8521 atattttttg cacctgtatt tttcatactt ggtggaataa tatctaataa atctaattat
     8581 atgtcaacta aaaatagctt gattggattt cttatatcat tcttacttat gttatgtgag
     8641 ggaatgtttt tacataaact aggggtacag cgtcatgata gcatgtatat tatgttattg
     8701 ccatgtatgt atttcttatt tacagcctta acatttttga aaggttctcg agctaggtta
     8761 ttgcgaacat catcattaat tatttatatt atacatccta tgatgattgt tgttattcgt
     8821 atgttttcta aaatattcgg aatacaaact gtcttggttg acaatagcct tattcattat
     8881 atagtggtaa gtatagcatc ttttatagct tctataatta ttatattgat tcttaaacct
     8941 ctaaaaaaag acttgaaagg agaacataag gtaaatacca atagagcttg gattgaaatt
     9001 gatttaaata atttggaaca taatgtaaag atgatacaaa aagctatgcc agaagattgt
     9061 gaattgatgg cagttgtaaa agctaatgcc tatggtcata gtgcttttga agtagcgact
     9121 tgtgcaaacc aaataggtgt aaaagcattt gcagtagcaa caatagatga ggggattgat
     9181 ttacgtcgat atggaatact aggagaaata ttgattcttg gatatactaa cccaataagg
     9241 gcaaaggaac ttcataaata caatcttaca cagagtgtta ttgaccttaa tcatgctatc
     9301 tctttaaaca aacagaaata caacataaaa tcacacatta aaattgatac aggtatgcac
     9361 agactgggtt ttgatgttaa tgatgtgagg tcaattttaa aggcatttaa attaaaatac
     9421 ttagatatat gtggcatatt tacacacttg tgtgtttcag atagtctgaa agatgaagat
     9481 gtagacttta caaataaaca aattgaatgt ttttataagt taattgattt tctgttaaaa
     9541 aaaggtataa aaattcctaa agtacatatt caaagtagtt atggtttttt taattatccc
     9601 aatcttaagt gtaattatgt aagaattgga attgcattat acgggatatt aagttctcca
     9661 aatgatacga ctaaattgca acttgattta cgacctgttt tatccttaaa atcacaagta
     9721 attcttatta gaaaaataca aaaagatgat agttttggat atggaagagt ctttattgca
     9781 agcagagata gcgtaattgc tatattatct attggctatg ccgatggtat acctagaaat
     9841 ttatcctgtg gaaaaagtta cgtaataata aatggttgta gagcagcaat agtgggtcga
     9901 atttgcatgg accaacttgc tgttgatatt acagatatac ctaatgtaga agttggtaat
     9961 acggcaataa taataggaag agataattta tctgaattat ctgcttctga agttgcaaat
    10021 aactcatgta gtatatcgaa tgaattattg agcagagtag gaagaaggtt aaatgttata
    10081 aagaagtaac tcaattaaat agtaatattt gttgtactgt gtaatttatt ttactctgac
    10141 tagacatttt tagtaatatt atgtctaata tatatttttt ataacttaca tgacaattta
    10201 agttataaaa aggtgatgtt tcagattttt attttgaaac atcacctttt ttgttataat
    10261 atataataaa ataattatac cttaactgta aacttatata gattatttac tttttaatat
    10321 tagattcatt ttttgagatt ggtatataat aaacatatag agtatataat aatagttaaa
    10381 gttaaaaaag gggaaaagca atatgcagta tatacaagaa ctacttgatt tgactcaaaa
    10441 ttattgggtg ttagccacaa taatcggctt attaagtgca tttatagaaa gctttatacc
    10501 agccttacca cttgttgcta tagttacagc aaatgctgca atacaaggat tatttattgg
    10561 atgtttgtta tcatggattg gctcaggact tgggacaact tcattattct tattaataag
    10621 cagatttaat gatagtaagc tttttaataa gttgagaaat agtaaaactg aaaaagcaat
    10681 aagttggatg gataaacagg gatttaagtt attatttata gcgtatgcct gtccatttat
    10741 gcccggttgt ttagttacta tcgcatcagc ttttggtaaa aaagatttaa aagattttgt
    10801 gccagcaatg ttggcaggaa aatttgtaat gtttatagtt ataagttacg tggcaagtga
    10861 tatagaaggt tttataacaa gtcctttaaa gatagcatca tttatattat tggtgttttt
    10921 gtcatggaag attggaaata gagtaaataa aaatcttgaa aatcataatt atgatttcca
    10981 tcataaaaaa catcacaatg ataaagatga taaaatgatt tagcttgtac gaaataaaac
    11041 gtatcttaaa atgatttagt act
//
LOCUS       DAYASV010000322         9929 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.guided.139, whole genome shotgun sequence.
ACCESSION   DAYASV010000322 DAYASV010000000
VERSION     DAYASV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.guided.139"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI68_003901"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="ACGI68_003901"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HFH0140259.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNNKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDVNDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="ACGI68_003902"
     CDS             1575..2258
                     /locus_tag="ACGI68_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0140260.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="ACGI68_003903"
     CDS             2255..4915
                     /locus_tag="ACGI68_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFH0140261.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIKLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPVE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKNIASSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYDTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="ACGI68_003904"
     CDS             complement(5096..6277)
                     /locus_tag="ACGI68_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0140262.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVVYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="ACGI68_003905"
     CDS             complement(6277..6939)
                     /locus_tag="ACGI68_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0140263.1"
                     /translation="MAKILLLEDNDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DN"
     gene            complement(7071..7709)
                     /locus_tag="ACGI68_003906"
     CDS             complement(7071..7709)
                     /locus_tag="ACGI68_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0140264.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="ACGI68_003907"
     CDS             7888..8493
                     /locus_tag="ACGI68_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFH0140265.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMMKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="ACGI68_003908"
     CDS             8997..9746
                     /locus_tag="ACGI68_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0140266.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KSDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..>9929
                     /locus_tag="ACGI68_003909"
     CDS             9743..>9929
                     /locus_tag="ACGI68_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0140267.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPG"
BASE COUNT         3729 a         1203 c         1541 g         3456 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacttaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaataa taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtactacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaagga gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atgtaaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataaa
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggagatag aatcctaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcaattagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctgtagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggt tgactgaaaa agatgcttca aaagaacttg cgaaaaatgg
     3901 aggatttaaa aatatagctt catctaaaaa gtactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttatga tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtgcagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaggtaa
     5701 ttggagtctt aagttgatga gcaatatctg caaggttatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatac aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ttgtcctcca ttctatatcc
     6301 tatgcctctt aatgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggattgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca ttatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttc atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcctta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatatttatg taaatataat atttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagatgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatgatgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 gtagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 aattttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtagtagtaa
     8701 ttattaaata agataaaaaa taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctctatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 gtgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaactcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggat
//
LOCUS       DAYASV010000323         9743 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Clostridioides difficile isolate ST8-SSI-h060-19
            SAMN40601768-rid21578083.guided.143, whole genome shotgun sequence.
ACCESSION   DAYASV010000323 DAYASV010000000
VERSION     DAYASV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN40601768
            Sequence Read Archive: SRR28438951
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-MAY-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002157707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002157707.1
            Genome Coverage       :: 197x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 16:24:31
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,923
            CDSs (total)                      :: 3,843
            Genes (coding)                    :: 3,813
            CDSs (with protein)               :: 3,813
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 3, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5 (5S)
            partial rRNAs                     :: 3, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 7 of 30
            Pseudo Genes (incomplete)         :: 22 of 30
            Pseudo Genes (internal stop)      :: 11 of 30
            Pseudo Genes (multiple problems)  :: 8 of 30
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN40601768-rid21578083.guided.143"
                     /isolate="ST8-SSI-h060-19"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Denmark"
                     /collection_date="2019"
                     /collected_by="Statens Serum Institute"
     gene            <1..450
                     /locus_tag="ACGI68_003910"
     CDS             <1..450
                     /locus_tag="ACGI68_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140268.1"
                     /translation="ETVDTLRLTEIFSSENIEDIKFIKDGTKKLEISIEKVVKFKRRK
                     KRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIFEDEDVIINKKVLKFPKSW
                     TKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVIILYYIYNH"
     gene            complement(778..1545)
                     /gene="aroD"
                     /locus_tag="ACGI68_003911"
     CDS             complement(778..1545)
                     /gene="aroD"
                     /locus_tag="ACGI68_003911"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFH0140269.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(2217..3053)
                     /locus_tag="ACGI68_003912"
                     /pseudo
     CDS             complement(2217..3053)
                     /locus_tag="ACGI68_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3124..3792
                     /locus_tag="ACGI68_003913"
                     /pseudo
     CDS             3124..3792
                     /locus_tag="ACGI68_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3919..4539
                     /locus_tag="ACGI68_003914"
     CDS             3919..4539
                     /locus_tag="ACGI68_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFH0140270.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            4695..5477
                     /locus_tag="ACGI68_003915"
     CDS             4695..5477
                     /locus_tag="ACGI68_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HFH0140271.1"
                     /translation="MTKTQQKMIQNVLEFSKRDSRIIGLAISSSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVANHEYKSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            5602..6528
                     /locus_tag="ACGI68_003916"
     CDS             5602..6528
                     /locus_tag="ACGI68_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140272.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKRERCLISGIGLERFLVAVS"
     gene            6542..6991
                     /locus_tag="ACGI68_003917"
                     /pseudo
     CDS             6542..6991
                     /locus_tag="ACGI68_003917"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            7405..7758
                     /locus_tag="ACGI68_003918"
     CDS             7405..7758
                     /locus_tag="ACGI68_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFH0140273.1"
                     /translation="MRNYARDIYDENKRRNDYLVSLESLSLAELSKLSILDDYDSNYI
                     CMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFFNMTNADIATLMDLAENTIHYH
                     KTNALKELKELMQEH"
     gene            7760..7990
                     /locus_tag="ACGI68_003919"
     CDS             7760..7990
                     /locus_tag="ACGI68_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0140274.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINVILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(8178..8315)
                     /locus_tag="ACGI68_003920"
     CDS             complement(8178..8315)
                     /locus_tag="ACGI68_003920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0140275.1"
                     /translation="MVFQPIPFLWTEPCHTGVSLYCGTGYGKTTVPKVIHKSLYSSSL
                     D"
     gene            8380..8583
                     /locus_tag="ACGI68_003921"
     CDS             8380..8583
                     /locus_tag="ACGI68_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0140276.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKRADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDVI"
     gene            8661..8993
                     /locus_tag="ACGI68_003922"
                     /pseudo
     CDS             8661..8993
                     /locus_tag="ACGI68_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
     gene            9086..9718
                     /locus_tag="ACGI68_003923"
     CDS             9086..9718
                     /locus_tag="ACGI68_003923"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFH0140277.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         3509 a         1438 c         1554 g         3242 t
ORIGIN      
        1 gaaactgtag atactttaag attgacagaa attttttcaa gcgagaacat agaagatatt
       61 aaatttataa aagatggtac aaaaaaatta gaaattagca tagagaaagt tgtgaaattt
      121 aaacgtcgaa aaaaaagaaa ttatgtttgt tgctattgcc ctgatatgta tagagacata
      181 aaactcgaca aagaatctat caataagata tacaacagaa aaataaaaat agaaagagaa
      241 gttaatattt ttgaagatga ggatgttata ataaacaaga aagtattgaa gtttccaaag
      301 tcttggacaa agaatatgca aaaatattgg ttaagtgaaa ataagtatcc catacattct
      361 actgtaattg atgatgatag atataaatgt tgtaatgtac aatatacaaa aaatagagtg
      421 ataatattat attacatata taaccattaa tatacataat tacaaaaaaa taatgacaaa
      481 tatacataat acaaatatat cttctacttt agataaaaca gttattatga aaacttacta
      541 tctataaata atagaaatgc tagtatacca tctataatct aatgtctagt aggttttaat
      601 ggcatatttt aaatatgcca tagttgattt ataagatttt ctatatcact ttcaaaatac
      661 acactaaata gttgcttttc agatataatt gcccaattta aattttacta tctgaaaagt
      721 tttttatatt taatttatat cttcaaaaat accgtctaat atttctatat aattaattta
      781 attaatactc ttatgtaata gattaagtac tgaatttaac tctttaaatg atatttgccc
      841 tggtgctgaa acactttttg cagctccaaa agtcaatgca gaaccaaata tttcaccaca
      901 caatctactt ataacaccca ttcctgacat tgacattgtt attattggtc tatctgcata
      961 aattttaaac atttcatttg tagcttcaag tagcactaac acatcttttt cattttgcgg
     1021 cataacagct attttgggta aatctgcgcc caattcctgc attctacata aacgagatac
     1081 aatctcttct ttttttggag ttttattaaa atcatgatta gatataatta ctttcacttc
     1141 ttttttatga gcaaaattaa ctacttcatc tatgacctca tctcccataa ataactctac
     1201 atctattaaa tctactaaac ctgtattaga aatttcttta ttcaaagtag tataataatc
     1261 tcttgatatt aatttttctc ccccttctac tacacttcta aatgtgaata ataaagggat
     1321 atcatgtata taacttctta attcatagag aacttcctta acttctttaa tattttctac
     1381 attctcaaaa aaatcaactc gccactctat aatatccaaa catgcatctt ttaactcttt
     1441 agcctctttt ataatatctt ttttattttt cccaataatt ggtacacaaa ttttaggtct
     1501 accttctcca atagtaatat tttttacttg aacttttctt ttcatattct cctccataat
     1561 cagaaattct ataggacttt aaattctatt ttctgcacaa tttaattata cattaaaaat
     1621 actatacatt ataaaaatac taatttaaaa ataattttta ttctaattct taaaacaatc
     1681 ccacatattt tagaaaaggt actctataat atacaaacac ttctttttta cctaaattta
     1741 ttttaacatc ctataatttt cacaatattc tttaaatttt actcatataa acaatattat
     1801 tctggatgct aatagaatta atctataaat catagtataa ttatgcagaa ataactcgaa
     1861 tttcactact tatgaagaga ttgaatatta atgaatctaa atcatttgca ttacttaaga
     1921 gttttgacta aagttgaaca ccatacaaaa gtagctactc aattgtttat accccaacca
     1981 agcttaagta atgttatata gattctcgaa aaagaatttg gtatcttttt atttgaaaaa
     2041 cttatattaa atttgattat cttttcataa gttggtaaat gtcgatatat cctgtatcta
     2101 caaggagtat cggcattttc tttttggaag atactttgca taagttggca tttaaccgta
     2161 taagaatgcc atcaaaagta gtaaataagt agtaaactct agtcatacta ctaggattat
     2221 aaccgttcca tttcagctct cgaagaacta aatgaaccat gtgcataata gccaagcgtc
     2281 atagtaatgt ttttatgccc cataatatat tgcaatatat taggactcat tcctttgtta
     2341 gccatatttg tacagagtgt atgcctaaag gtatgtggcg taatctttgg tagcttgtcc
     2401 tcatgggtct tattatactt cttgactaac gctcttaaca tgctttgata atgagttgct
     2461 tgttttggta atccatcttg tttaagaaat aggaagttct ggtaaccatc aacaatgatt
     2521 ggttctgctt tagcgttctt acgccgttcc acgatacgtt caaatgattg ataagctctt
     2581 tcagtcattg gaatttctcg cttaccctgt gtggtctttg gaacttctac ataatagcca
     2641 atcttagtat cactcaacaa ttgatggtct accataatgg ctttattttt catatccaat
     2701 tctaaagtca aaccgcataa ttcagaaatg cgtagtcctg tttctaagag gataacaaca
     2761 tcatcataat atttacaata agtcttatcg ctccccataa aggataataa gcccatttcc
     2821 tgttctggtg ttagagcgat ttttggttct gttgtatctt ccaaaacatc acttaactaa
     2881 aaattaaatg gattctttct aatacagtcg tcttgaattg ccatgaaaaa cgacgctttc
     2941 agtgagcgct tataattgtc aatggattta taagcacacc catcttcact cattcggata
     3001 gcccagcttt tagcgtctga taatttaacc atatcaatgc tttttgcacc tagtgggtca
     3061 ttgatagtat ctatattttg atttataatt tatacatttt tttaaaaaca gacttttaac
     3121 tgtaaagaat ctatgtataa acttagctat gttgtccgtg tatctacaaa gaatcttgac
     3181 gagctaaaaa ggcgttgtga tgaagtcaaa gacttttacg acgacttatc aatcaagctc
     3241 gtgcgtccct ttggggatat gttgggattg catggcgaat ttattcctgt cagcaaacgt
     3301 tacatcaatg attatattca atatgtcact tcggattttt tggctggact tggttttgga
     3361 gcgacacaaa cccttggcga acatgaggat atctatcttg gctacaatct ggacactgga
     3421 aagaatgtct atctaaaacc tgcccttgct agtcaagggg taaagggttc tattaccaat
     3481 gcgttagcgt ctgcgtttat cggttcattg ggtggtggta aattattcag taacaatatg
     3541 cttgtttact atgcggtact attcggcgga caggcggtca tagttgaccc taaagcagaa
     3601 cgcggcaaat ggaaagaaac gctaccagaa atagcccatg agataaacat tgtcaattta
     3661 accagtaatg atgaaaacaa aggattgctt gacccttacg tcattttgag ccgtaaaaag
     3721 gattctgaaa gtttagcgat tgatatccta acattcctta ctggtatctc cagtcgtgac
     3781 agtgataagt ttttgcagaa aaatgacaac tggaaaataa taatggaata gtttctatgt
     3841 aaatatattt ttactatctt acctaactaa ttgaaagatg atataattta atcaaagatt
     3901 tggtaaggag ggatgaatat gcatttaatt gattttatga atgaagatat taaaaagaag
     3961 cttgttaaaa aaacgtataa aaagaaagag actattcttt ttgctgagca agaaaatgaa
     4021 tatgtaattc ttatgattga cggtattgca gaagcattca tcttaaatgt gaaaggaaac
     4081 atctcaacga ttcatcttta tagaagtggt agtttttttg gagaaatgga acaatttaat
     4141 gatgggaaaa aacccgttga aattgtcgct ttcacagatt gtaatgtata taaacttcat
     4201 agagatgatt ttttaaactg gttaagggga gattttgaag ctacaaaatt tctgattcga
     4261 gaaatatcta gtaaattagt tgtaaatgct gaattaattg aagaattatc atctttaagt
     4321 gttaaagaac gacttctgag gtgtatatcc ttacactatt atagaagtga attaaatctg
     4381 cttaccaaaa aacagcttgc tagcgaagta aacgctccaa ttagaagcat taatcgtgct
     4441 ataaatgaat gtgtacaaga acatctaatt gcttatgaaa acaaaagatt tagtgtaatt
     4501 aatcagcgag aggtattaaa gaatcttcct acaacataag tataatttca aaaaagtatt
     4561 gaataataat tattcaatgc ttttttattt cgggtcaagt gacctatttt tttttatttc
     4621 ttcattatat aatactccta tgcataatta tgttggattg atagctaata aaagacagtg
     4681 agaggtgtta tattatgact aaaacacaac agaaaatgat acaaaacgta ttagaatttt
     4741 caaaaagaga tagtaggata ataggacttg caattagcag ttcttatatc acaaaatcgt
     4801 tagatgaata ttctgatttg gattttctga ttgtagcaga tgataaatcc tatgaagaag
     4861 ttatcgaaga gagattactt attgtggaac agtttggaac attagtttct gcttttactg
     4921 gcgaacacgt tggagaacct cgattgataa tttccctcta tgattcccct gttcttcatg
     4981 ttgattttaa gtttacaacc ttagatggtt tactagatag ggttgaagat tcagctattc
     5041 tctatgaaga aaacaactgc ataacagaaa tttattctaa aaaagaagca tacttcccaa
     5101 ctccagactt acagtggatt gaagatagat tttggatttg ggttcattac gcttgcacta
     5161 agattggacg tagagaacta tttgaaacaa ttgatttttt atctttttta agacaatcag
     5221 tacttgcccc attaatacag cttctcttag gtaaattacc tcgtggagtt agaaaaattg
     5281 aattggacgc accccaatat attgaagcat tagaaaagac ggttgctaac catgaatata
     5341 aaagctgtgt taatgcattg caggctacta tcaacctgta tcttgagtta agagaggaat
     5401 tatcacaaag tcctattgtc cgaaaggttg aagctgaaag ggttgcatta cagtattttc
     5461 aagatttaat caaataaacc caaagtaaaa aaatattgaa tagtaaatat tcagtgtttt
     5521 ttcatttttg gtcaaatgac ctagattagt tgttgtaaca attctataat tattttgaaa
     5581 gtaacagtat ggaggataac tatgagtgat attttaagta gaattacaaa taaaattggg
     5641 gataagaata tagttgataa gttatcagct ctatcaaaat cagatttgaa ctcacttcta
     5701 ttagaagtgt ttgatagaca agcaaatact ttaactgcaa ctgatattct aaagtcatac
     5761 caactaaata gatttactat ccctagcggt atagatccag aagaaattca tgctttggaa
     5821 tcaaaattat tgagaaaagc attcaatatg gatatcaaga cgattatgct ttctccctct
     5881 gccccattag gtagctgtag cgtttttggc tccgtagatc aatataacgt agtaagtgct
     5941 ctacgaggga cagaaacact tgcagacccc tcaaatatgt tggcaataat tattgctgat
     6001 aaattaaaaa gaaaagaaga aaataatacc atacctatcc acatggctac tactgctagg
     6061 gtaataagag cacaaaattt tgtaggtaaa ggtttatatt cacattttgg cttatattgt
     6121 atggtttcat ctggtataga tacagggtct tacacttgtg aaaaaatgtt gttagaaaag
     6181 cacttaaact attataaaga tatattaagt gaaatagaaa atgttcgttt ctctattatt
     6241 ctacgcaaaa gaaacggata caaagataat gacggatttt tcgacagaat ggttgaaact
     6301 atcaaactta tttttcctaa tatagagtta tcaattcata atgatgatgt tgataataat
     6361 tattataaag gtatcaattt caaattatcg gtcaaacaag gaaatgaaac cgttgaaata
     6421 gttgacggtg gatttgttga ctggacttat caaatgcttg gaaataaaag agaacgctgt
     6481 ttgataagtg gtattggctt agaaagattt cttgtagctg tttcataaaa ggagaagaaa
     6541 aatggaactt atagagtgga attatgctta tattaccgat ttagttgagt ttgcgaatga
     6601 taaagaaatt gcaaagaatc ttcgtgacaa cttcccccac ccttataggg agcaaaacgc
     6661 tagagatttt gttagttttt gtttggcaac ttcagatgtt aaacaactta gctatgctat
     6721 tttttatcaa gaaagggcta ttggaagtat cagcctaaca tttgaagaag atacctctat
     6781 taataaaaat gctgaattag gttactggct tgctaggaac tattgaggca aagggatagc
     6841 aacaaatgca gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc ttaacaaaat
     6901 tcatgctaat gtatattcct acaatagaga gtcaagtaaa gtattggaaa aatgtggctt
     6961 taagattgaa gaatactgtc acaattcata ataaagaata gaaacatgat tgattgccat
     7021 gtatatagtt taattcgacc gcaatagcat ttattagaat taacctactt tttaataaca
     7081 gaataagttt ttcccctcta ttaatttctt atgcatcttt gtatttttat gaaaagctag
     7141 ccaatgcata tttccataca tcaatatggt ggtggcttac aaccaatata aattaaatac
     7201 gttttaaaga atacacattt ttgccctata ggcagagtgt gtattttttt ctcttttttt
     7261 tgaaatttat cgaagattta acgccctccc accgtgatag gagtaagcaa ggttatttaa
     7321 ctccttgcga tggaaagagg gtgaaatctt atggttgatg aaaagacaat tgaacatcaa
     7381 ttcgacagtt tctgtaaaac agttttgcgt aactatgcta gagatattta tgacgaaaat
     7441 aaacgcagaa atgattattt ggtatctctc gaatctttgt cacttgcaga attaagtaaa
     7501 ttgagtattt tagatgatta tgattctaac tacatctgta tggtttctta cgactacaac
     7561 atacgaattg aagatgtatt aatggcgcaa gcaatcggaa aacttacaaa aagaaaacaa
     7621 gatataattc tactttcatt ctttttcaac atgacaaatg ctgacatagc aacattgatg
     7681 gatttagcag aaaataccat acattatcac aaaacaaatg cactaaaaga gttgaaagaa
     7741 ctcatgcagg aacattgaaa tgaaacctac taaagaagta ttacctttta caattatttt
     7801 acttgctgta catggcgatg taaatgcaat aaacgtaata cttaaacatt ttgaacacta
     7861 tatcattcgt ctttcacaga aaactttatt tgatgaatac ggaaatccct atattcatgt
     7921 agaagaagaa acaaagcgaa tgctaggaac aaaactaatc actgctattt tgagatttaa
     7981 tttgtcttaa tctcgccctc tctccatgta tcaagctatt tggtacatgg agaatcacta
     8041 attatttgtc tataaaagcc cacaagctat tgtgattttt tataggcaaa tcaatgccga
     8101 ctgttctttg acaactgaat aaagtagata gatacgtttg tgatacagat agccaacaaa
     8161 tactatacgc catgacctta atcaagagag ctactatata gtgatttgtg aataaccttt
     8221 ggaacggttg ttttgccgta acctgtacca cagtataatg atacacctgt atgacacggt
     8281 tctgtccata agaatgggat tggttgaaat accaatggag cttatcaaag ccatctatct
     8341 acttctgaac tacgaacata gaaaggagtg atacattata tgaacaatac tgatgtacct
     8401 atttgggaaa aatatacctt gacgattgaa gaagcgtcaa aatactttcg tattggagag
     8461 aaaaaattac gtaaacgtgc tgatgaaaac cttgattctg gctgggtcat tatgaatagc
     8521 aatcgcattc aaataaagag aaaacaattt gagaaaatcg ttgatacact tgacgttatc
     8581 taatgaaatt gagccttata tgttataata ttcatatgac gtatcaagct caattcggtt
     8641 attgagagga gcttcttatc atgtcaactg aaaaaagacg agataataaa gatagaattt
     8701 taagaactgg agagagccag agaaaagatg gaagatacgc atataagtat gtatattcat
     8761 ttggtaaacc acaatttgtt tactcttgga aacttgtacc tactgataaa cccccaaagg
     8821 gaaaacgtga tgatatatca ttaagggata aggaaaaagc aatacaaaat gaccttgatg
     8881 atagcattga cactattggt aagaaaatga cagtttgtca gttatacgct aaaaagaatg
     8941 gactaagaaa gaatatccga cttaacacta aaaaaggacg ccattatctt atgaagattc
     9001 ttaacaagga gtgattctgt gaagagatgt attgtagtag tggaatagtc aaaaacataa
     9061 tatatattaa aggagagatt taaatatgac tattccaagt tcaaataagg tatatccaag
     9121 aagtaatgat tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt
     9181 tggagattac acaatatata atgactttta taatgaccca agagattttg agaaaaacaa
     9241 tgtattgtat cattacccta taaacaatga taaattaata attggtaagt tttgctcaat
     9301 agcatgtaat gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac
     9361 atatactttt cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc
     9421 ttgggacaat aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat
     9481 aattatgtca ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac
     9541 taatgacgtt ccaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag
     9601 atttaataat gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa
     9661 aaaaattcaa tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata
     9721 ttttataatt tatttatatt aaa
//
